LOCUS       DASTSF010000001         2321 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_668722,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000001 DASTSF010000000
VERSION     DASTSF010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2321
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_668722"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="VFU15_00005"
     CDS             <1..235
                     /locus_tag="VFU15_00005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716178.1"
                     /translation="SYNPNPTGQYCMAGNVSEMVWTSDPANPSVHGAARSMGGSWYSY
                     VDNVHIEASEQYVGVTDARAYIGFRPLMTFAAK"
     gene            425..2194
                     /locus_tag="VFU15_00010"
     CDS             425..2194
                     /locus_tag="VFU15_00010"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEU4716179.1"
                     /translation="MNAQEFPQAWKSSFSSSPSKLTDFSADGSLVLGASETSAQMLDG
                     NTGASKWDIKFKDKFGVKTFDKVRWNDASGVVVLFNADSKKEKGLQVFIDDQTGNELW
                     RSSDYGGTDAQSYYNALHGLLDNYFESVKGFAVLNNTKSQLDMVEAKSGKALWSVPVT
                     GNVSVGKAEGYNLCFTDDGNEVKYYDAETGKPVASVDFSKYALKSDRKTLYGGMAVSE
                     EQDRMATMTYQKRLVAGATGSKRKITVTCKKLSDASTQWATTFEGIVVTTLTTEEDLV
                     SIDIVGDKVFAMYEGITVLDLATGKILWSADFDNSDVSVGLKAKQEIGISAMPFVDGN
                     AVYVVDLKKDNDIKKYDAATGKILWESPKMGKDPVVPNIVVADGVLIAQLGGRINVQT
                     YIPGSNGNPDVYKSEYKFVGPYGLIAYGTDNGQVLWQTDKMKDVLKDKFSDRITDVQV
                     ADGKAFVCSDKNLFCLDPKTGQPSFTTPLGDLKIGGPLQLTLDKKGGRIYVICDKGIA
                     AFGMSDGKNIYATKVGDILSDFSVGDNYFVWIGEDEFAGFDLATGKVKGTFKGQTHPR
                     LTPDGNYLFNFDGSKVQKYKINP"
BASE COUNT          619 a          604 c          619 g          479 t
ORIGIN      
        1 ttcctacaac ccgaacccga caggacagta ttgtatggcg ggcaacgttt ccgaaatggt
       61 gtggacatcg gacccggcga acccttcggt tcatggtgca gcacgaagca tgggcggatc
      121 atggtacagc tacgtggata atgtgcacat tgaagcaagc gaacaatatg tcggcgtgac
      181 ggatgcgcgg gcttacatcg gtttccggcc gctgatgaca ttcgccgcga aataagcagc
      241 acatccgcat cgcattcttt ttcccggttg caaaacgacc gggatttttt atgcgtactc
      301 acccgaaagt ggtattgccc ggaacctgca tccggggcag atttgcatgt tcatcttact
      361 taaccaaaca accactatga aaaagatcag gaacctgatt actgtactat tgttatccgc
      421 ctctatgaat gcacaggaat ttccgcaggc gtggaaatcc tctttcagct cttcaccttc
      481 gaagctgact gatttttccg ctgacggttc gctggtgctg ggcgccagtg aaacttcggc
      541 acaaatgctg gacggcaaca ccggtgcatc gaagtgggat atcaaattca aggataaatt
      601 cggcgtcaag accttcgata aagtgcgctg gaatgatgcg tccggcgtcg tcgtgctgtt
      661 caacgccgat tcaaaaaaag aaaaaggcct gcaggtcttt atcgatgacc agaccggcaa
      721 cgagctgtgg agaagcagcg attacggggg aacagatgca cagagttatt acaatgcgct
      781 gcacggcctg ctggacaatt atttcgaatc ggtcaaaggt ttcgcggtgc tgaacaacac
      841 caaatcacag ctcgacatgg tggaagcgaa aagcgggaaa gcgctttggt ccgtcccggt
      901 tacgggaaat gtcagcgtcg gcaaagccga gggctacaac ctctgcttca ccgatgacgg
      961 caacgaagta aaatattacg acgccgaaac cggtaaaccc gtggcttccg tggatttttc
     1021 aaaatatgcg ctgaaaagcg atcgcaaaac actgtacgga ggaatggccg tttccgaaga
     1081 gcaggaccgc atggcgacca tgacctacca gaagcggctt gtggccggcg caacgggcag
     1141 caaacgcaag atcaccgtca cctgcaaaaa attatcggac gcatccacgc agtgggccac
     1201 cacattcgaa ggcattgtcg tcactacgct cacaacagaa gaagaccttg tcagcatcga
     1261 catcgtgggc gacaaagtgt tcgcgatgta cgaaggcatc acggtgctcg atctcgccac
     1321 cggtaaaatt ctctggagcg ccgacttcga caattccgac gtgagcgtcg gcctgaaagc
     1381 aaaacaggag atcggcatat cggccatgcc ttttgtggac gggaacgcgg tttacgtggt
     1441 cgacctgaaa aaagacaacg acatcaagaa atacgatgca gcgaccggga agatcctctg
     1501 ggaatcgccg aaaatgggaa aagacccggt cgtgccgaac atcgtggtgg cagacggagt
     1561 gctcatcgcg cagcttggcg gacgcatcaa cgtgcagacc tacattcccg gctcaaacgg
     1621 caacccggat gtttacaaaa gcgaatacaa attcgtcgga ccgtacggac ttatcgctta
     1681 cggaaccgat aacggccagg tactctggca gacggataaa atgaaggacg tgctgaaaga
     1741 taaattcagc gaccgcatca ctgacgtgca ggtggccgat ggaaaagcat tcgtctgcag
     1801 cgataaaaat cttttctgcc tcgatccgaa gaccgggcag ccttctttca ccactccact
     1861 cggcgacctg aagatcggcg gtcctttaca gctcacgctg gataaaaaag gcggccgcat
     1921 ctacgtcatc tgcgacaaag gcattgccgc cttcggaatg agcgacggga aaaatattta
     1981 cgccaccaag gtcggcgaca tcctttctga tttttccgtc ggcgataatt atttcgtgtg
     2041 gatcggcgag gatgaattcg ccggcttcga tctcgccacc gggaaagtga aaggaacgtt
     2101 caagggacaa acgcatccgc gcctgacgcc cgatggaaat taccttttca atttcgacgg
     2161 aagcaaagtc cagaaataca agatcaaccc ctgaccgggc ggacccctgc gtaaccggcg
     2221 tcacatgctc cggcatgtga cgcttttttt cgctgcgggg gaatttatgg agggtacata
     2281 ccccctttat ggacaacctt gcaggttata acctgcaagg t
//
LOCUS       DASTSF010000002         6620 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1112776,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000002 DASTSF010000000
VERSION     DASTSF010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 6620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6620
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1112776"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..779)
                     /locus_tag="VFU15_00015"
     CDS             complement(<1..779)
                     /locus_tag="VFU15_00015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716180.1"
                     /translation="MKRLTLICLLLLSTFLAKATHIVGGSLTYEHLGGATYRITLKMY
                     RDCRAGNAAFPGSVTVQVRDQNGNGFSPTKDFSMNFTTSTPVNPYIDTCAVNPGLCLE
                     EAIYTRVVNNLPPLAGGYFMFIRYCCRNSTLNNINNPLSTGDCWYTHIPDMSTLITNS
                     SPVWVNPPPVFVCQAEPINFDFSATDADGDSLVYSFYAPYDSITPTISGGNATFTPVS
                     YQAGYGPNNPCGGPNLTINPQTGFMAGSPPNVGQFVGGVRC"
     gene            complement(886..1359)
                     /locus_tag="VFU15_00020"
     CDS             complement(886..1359)
                     /locus_tag="VFU15_00020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716181.1"
                     /translation="MKSTALPLIIAFLVLAAAGAIVSWAFFHRDRQLQLVDIPPSYLS
                     SVDTSRFSVQPLVLTREFPDDSVLLIFPANTSLQHIFVYDQKNYNRLLFGDHHGMPLF
                     FADAEGLSAMKGKPVINLSDHPGGKYFIHVTSCNFGGFLQIELEDMGSGDDASAK"
     gene            1467..3983
                     /locus_tag="VFU15_00025"
     CDS             1467..3983
                     /locus_tag="VFU15_00025"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HEU4716182.1"
                     /translation="MHSRKILSILAGVFASSLVFSQVNVQWVARYTTAGNNIDRAKSM
                     VVDASGNSYVVGTSWNGTDFDIVTTRFDANGNFVWATPYNGPGNGYDEARAIVIDNAG
                     NVYVTGYSAGAASNYDIVTIKYNSAGSQQWATRFDGTAAGFDEGYDIAVDGSGNVYVT
                     GGTTSTTGGNNYVTIKYNPAGVQQWATVYNNNNNNTECAYAITLDGSGNVYVTGSSYG
                     GSANDNDMATIKYNNSGTQQWAKRYNGPGSVYDAGSDIIVDGAGNVYVTGYARDLVGT
                     TNYSYATVMYNSAGTQQWASIYDGTGNDYDAANAITLTPAGNVAVTGHSIGTTSTAED
                     MATVLYNGTNGNVIWVRRFDGGAVQYDEGTAVSADSSNRIYVTGYSYAPGTNNNYLTV
                     KYEANGDTSWIVKYNGPGNNSDQAYSMALGPTSEVYVGGMSKGAGTNEDYAVVKYCQL
                     RADAGLDTAICLGASVQLNVTSSYMGISNIAWSPAGSLSQSNITNPVATPSVTTDYVV
                     ALTNTLGCTDLDTVRVTVIPLPGPQITSSGPTSFCIGGSVTLTANDTTGGNVSYLWSN
                     GDTTASTTVNSSGTYSVTITNGNTCSSQSTATVTVNPLPNISAGNDTNLCASSTINLC
                     ATGGTSYSWTPSFGLSDTTLACPVAGPTQSTTYIVYGTDANGCSSSDTVVVGLYPPPS
                     VPIISQNIAVLSSTPASTYQWYFGATAIPGATGQNYTPTQNGQYYVEITDANGCSAFS
                     APYTINDVGFADLSSGTNDLSIFPNPNNGTMTLDIPQNGQKGTLDVYSLAGQKVYSMD
                     LGAAAINHLDLQLSLDAGNYFVTVTLEDGTRMVKRIVIQN"
     gene            4053..4796
                     /gene="kdsB"
                     /locus_tag="VFU15_00030"
     CDS             4053..4796
                     /gene="kdsB"
                     /locus_tag="VFU15_00030"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013455330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HEU4716183.1"
                     /translation="MKIIGIIPARFASTRFSGKPLVDIGGKPMIVRVYEQAKKAARLA
                     AVYIATDDERIAEVARKHNCEFVMTSAEHPSGTDRCAEAVRKIEGSWDAVINIQGDEP
                     FIQPEQIDLLASLFTDATVEIGTLVKKLKDPSDLDNPNTMKVVLDNSGNGMYFSRSAI
                     PFVRNFPKAEWTGHHLFFRHIGIYGYRTQVLEKITKLPVGRLEKAESLEQLRWLENGY
                     RIRTALTDMETISIDAPQDIDKLRRAGLI"
     gene            complement(4882..6261)
                     /locus_tag="VFU15_00035"
     CDS             complement(4882..6261)
                     /locus_tag="VFU15_00035"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU4716184.1"
                     /translation="MYIHQFYTNCLAQAAYYVESDREALIIDPLRDPEPYIEFARSRG
                     ASIKFILETHFHADFVSGHLDLAAATKAAIVFGPDATPSYPAYIAKDHERLRIGKTEI
                     EILHTPGHTVESSCFLLYDEAKNPHAVFTGDTLFAGDVGRPDLLSGNLDAETLAAKLY
                     DSIQKIKTLPDDVIVYPGHGAGSACGKNIGKETMTTIGEQKKKNYALQEIPREVFVSL
                     VTNDQPAAPAYFFSDAKLNKSGYEPFEKVLSHGLHFVEAPEFDRMKREGLTILDTRDP
                     EEFAKGFVPGSVNIGLEGDFAVWVGTLFSFGTKFLLVTEKGKEKESVTRLARIGFDHV
                     EGILKGGMENWISGGRPADRIAQLSPMEFMQEAATGKYVVLDVRRKGEAEKKRLLHAS
                     NLPLAELKDKVHRLDPKENFILCCAGGYRSMIAASLLKAEGFDNVQNIAGGVSKILTE
                     IPELIEMEE"
     gene            complement(6300..>6620)
                     /locus_tag="VFU15_00040"
     CDS             complement(6300..>6620)
                     /locus_tag="VFU15_00040"
                     /inference="COORDINATES: protein motif:HMM:NF013411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HEU4716185.1"
                     /translation="GKPADKPGDPKNGMVIDFTDIGRVVRKNIIDVFDHALVLCQSDR
                     EHFPANELSGKVIYLPFPPTCEMLLLEFQRRLAAEENNFPVSGLRLDETATSFAEWHA
                     NGNL"
BASE COUNT         1600 a         1721 c         1808 g         1491 t
ORIGIN      
        1 tcacatcgga cgccgccaac aaattgtccc acattcggcg gtgagccggc catgaacccg
       61 gtctgcggat tgatcgtaag gttcgggccg ccgcacgggt tgttcggacc gtacccggcc
      121 tggtagctga caggcgtgaa tgtcgcattg ccaccggaga tcgtcggggt gatgctgtca
      181 taaggcgcat aaaaagaata cacgagcgaa tcgccgtcgg catcggttgc ggaaaaatcg
      241 aaattgatcg gttccgcctg gcaaacgaag accgggggag gattcaccca gaccggcgag
      301 ctgttcgtaa tgagcgtcga catatccggg atgtgcgtgt accagcagtc gcctgtggaa
      361 agcgggttat tgatgttgtt cagcgtactg ttacggcagc aataacggat aaacataaaa
      421 tatcccccgg ccagcggcgg aaggttgttg acaacgcggg tgtagatcgc ttcctcgagg
      481 caaagcccgg gattcaccgc gcacgtatca atgtaaggat tgaccggcgt ggaggtcgtg
      541 aaattcatgg agaaatcctt cgtcggcgaa aaaccgttgc cgttctggtc gcgcacctgc
      601 acagtgacag atccggggaa cgcggcgttg ccggcgcggc agtcgcggta catcttgagt
      661 gtaatgcggt aagtggcacc gccgaggtgc tcgtaggtta atgatccgcc cacgatgtgc
      721 gtggcttttg cgaggaatgt tgaaagcaaa aggaggcaga tcagggtaag ccttttcatc
      781 tttttgcgct aagtgcaggg attggtgtct gctgttggct aagatacgac aattgccgga
      841 agtgcaaaga caaaaggcct gtttctggta acaaacagtt gttgatcatt tcgcggaggc
      901 atcgtccccg cttcccatat cttccagttc gatctgcagg aacccgccga aattgcagga
      961 ggtgacatgg ataaaatatt tcccaccggg gtgatcggaa agattgatga cgggctttcc
     1021 tttcattgcg ctaagtcctt ccgcatcggc aaagaaaagc ggcatgccgt gatggtcacc
     1081 gaacaggaga cggttgtaat tcttctggtc gtaaacaaaa atgtgctgca gcgatgtgtt
     1141 cgccggaaag atcagcagca ccgaatcatc ggggaactcg cgcgtgagca ccagcggctg
     1201 cacactgaac cgcgacgtat cgacgctcga gaggtaggaa ggaggaatat caacgagctg
     1261 caattgcctg tcgcgatgaa agaacgccca tgaaacgatc gcacccgcgg cagcgagaac
     1321 gaggaaggca ataatgagcg ggagggcggt gcttttcatc tttggctaat gtcgcaatta
     1381 ctttcatcat ggacaatgga agaggcctta ggatcgcttt aattttatct ttctatcttt
     1441 ggtaaaaccc aaaggcaaac atcagcatgc attccagaaa aatcctttcc atactcgcgg
     1501 gtgtgttcgc gtcttcattg gtattttccc aggtcaacgt gcagtgggta gcccgttaca
     1561 ctacggccgg aaacaatatc gaccgggcaa aatcgatggt ggtggatgct tcgggcaaca
     1621 gctatgtggt tggaaccagc tggaacggta cggatttcga catcgtcacc accaggttcg
     1681 atgcaaatgg aaattttgtc tgggcaacgc cgtacaacgg tccgggcaac gggtatgatg
     1741 aggcaagggc gatcgttatt gacaatgcgg gcaacgtgta cgttacgggt tacagcgcgg
     1801 gtgcggcgag caattacgat atcgttacca tcaaatacaa ttcagcagga agccagcaat
     1861 gggctacgcg gttcgacggt acggctgccg gttttgatga aggctatgat atcgcggtgg
     1921 atggctcggg aaatgtatac gtcaccggcg gaacaaccag cactacgggc gggaacaatt
     1981 atgttactat aaaatacaac ccggccggcg tgcagcagtg ggcgacggtg tacaacaaca
     2041 acaacaacaa tactgaatgt gcttacgcca tcacgctcga cggttcggga aatgtttacg
     2101 ttacgggttc cagctacggc ggttccgcga acgataacga catggccacc atcaagtata
     2161 acaattccgg cacgcagcag tgggcgaagc ggtacaacgg acccggaagc gtttacgatg
     2221 ccggctcgga tatcattgtc gatggtgcgg gaaatgttta tgtgaccggc tatgcacgcg
     2281 acctggtggg aacaacgaat tacagctacg ccacggtcat gtacaattcc gccggaacac
     2341 agcagtgggc ctccatttat gacggaacgg gaaatgatta cgacgccgca aatgcgatca
     2401 cgcttacgcc cgcaggaaac gtagctgtga ccgggcattc catcggtacc acttccactg
     2461 cggaagacat ggcaactgtc ctgtacaatg gaacgaacgg aaacgtgatc tgggtgcgcc
     2521 gctttgacgg aggcgctgtc cagtacgatg aaggaacggc cgtttctgcc gacagctcga
     2581 accgcattta cgttacgggt tattcctacg cgccgggaac gaataataat tacctcacgg
     2641 tcaaatacga agcgaacggt gatacgtcct ggatcgtcaa atacaatgga ccgggaaata
     2701 attccgacca ggcgtattcg atggcgctcg gacctacaag cgaagtttac gtaggcggca
     2761 tgtcgaaagg cgccggaacg aatgaagatt atgcggtggt gaaatactgc cagcttcgtg
     2821 cagatgcagg gcttgatacg gcgatctgcc tgggcgcaag tgttcagctc aacgtcacca
     2881 gctcatacat gggaatttcc aatatcgcat ggtcgccggc cggaagcctg agccagtcca
     2941 acatcacgaa cccggtcgcc acgccttccg ttacaaccga ttatgttgtc gcgcttacga
     3001 atacgctcgg ctgcaccgat ctcgatactg tgcgcgtgac tgtcattccg ctgccgggtc
     3061 cgcagatcac ttccagcggt ccgacatctt tttgtatcgg cggctccgtc accttgactg
     3121 ccaatgacac aaccggcgga aacgtctcgt atctctggag caatggcgac acgacagcaa
     3181 gcaccaccgt gaattcttca ggaacctatt ccgttacgat caccaacggc aacacctgct
     3241 ccagccagag cacggccacg gtcactgtaa atccgctgcc gaacatcagc gcgggcaacg
     3301 acacgaacct ttgcgccagc tccaccatca acctttgcgc gaccggcgga acttcttatt
     3361 cgtggactcc ttctttcggc ttgagcgaca cgacgcttgc atgtcctgtt gccggtccca
     3421 cgcaatcgac gacgtacatc gtttacggaa ccgatgcgaa cggatgctca agctcggata
     3481 cggttgtggt cggattgtac ccgccgcctt ccgttcccat tatttcacag aacatcgccg
     3541 tgctgagctc gacgcccgcc tcaacgtacc agtggtattt tggtgcaacg gctattccgg
     3601 gcgctacggg acagaattac acgcccacgc agaacggaca atattatgtg gagatcacgg
     3661 acgcgaacgg gtgctccgca ttttccgccc cgtatacgat caatgacgtc ggctttgccg
     3721 atctcagcag cggtacgaat gatctcagca ttttccccaa cccgaataac ggaacgatga
     3781 cgctggatat tccgcagaac gggcaaaaag gaacgcttga cgtttacagt ctcgcaggac
     3841 agaaagtata cagcatggac cttggcgcag cggcaataaa ccatcttgat ctgcagcttt
     3901 cgctggatgc aggaaattat ttcgttaccg ttacgctgga agacggcaca cggatggtga
     3961 agcgcattgt cattcagaat taatttccgc gaacaggaga aagaggcatc atgcgatgcc
     4021 tttttttatg gattattgcc aacttagctg caatgaaaat catcgggatc ataccggcac
     4081 gttttgcttc cacgcgtttt tccgggaaac cgctcgttga catcggcggc aaaccgatga
     4141 tcgtgcgcgt ttacgagcag gcgaaaaagg ccgcaaggct tgctgctgtt tacattgcaa
     4201 cggacgacga gcgcattgcg gaagttgcac gaaaacacaa ctgtgaattt gtcatgacat
     4261 cggctgaaca cccgagcgga acagaccgct gtgcggaggc tgtgcggaag atcgaaggaa
     4321 gctgggatgc ggtgatcaac atacagggcg atgaaccttt catccagcca gaacagatcg
     4381 acctgctcgc gtcgcttttt acggatgcga cggtggagat cggcacgctc gtgaaaaagc
     4441 tgaaagatcc ttcggatctc gataacccga atacgatgaa agtcgtgctc gataactccg
     4501 gcaacggaat gtacttcagc cgttctgcga tcccgttcgt ccgcaatttt ccgaaagcgg
     4561 aatggaccgg gcaccatttg tttttccggc atatcggcat ttacggctac cgcacgcagg
     4621 tgctggaaaa gatcacgaag cttcctgtcg gcaggctgga aaaagcggaa agcctggagc
     4681 agcttcgctg gctggaaaac ggctaccgca tacgtactgc gcttacggat atggaaacca
     4741 tttccatcga tgcgccccaa gacatcgaca aactccgccg agccggactc atctgatcac
     4801 tgcgtacaat tttcgctgtg catattttac cggttgaaat cctgaagggt tcgccaggat
     4861 caacggaaac agtaccccgt tttattcttc catttcgatc agctcaggaa tttccgtaag
     4921 gatcttcgat acaccgccgg caatgttctg cacattgtcg aatccttccg ctttcagtag
     4981 cgaagcggcg atcatggaac gatagccgcc ggcacagcag agaataaaat tttctttcgg
     5041 atcgaggcga tgcactttgt ctttcaactc agcgagcggg agattggaag catgcagcaa
     5101 tcttttcttt tccgcttcac ctttgcgtcg cacatcgagc acaacatatt tcccggttgc
     5161 cgcttcctgc ataaattcca taggtgaaag ctgcgcgatc cggtcggcgg gacggccgcc
     5221 tgaaatccag ttttccattc cgcctttcag gattccttca acgtgatcga aaccgatacg
     5281 agcaagacgc gtgacggatt ctttttcctt tcccttttcc gtaacgagga ggaatttcgt
     5341 cccgaagctg aacagcgttc ccacccatac ggcgaaatca ccttcaagac cgatgttgac
     5401 tgaacccgga acgaatcctt tcgcgaattc ttcgggatcg cgtgtatcga ggatcgtcag
     5461 tccttcacgt ttcatccggt cgaattccgg cgcctccacg aaatgaagtc cgtgactcag
     5521 cactttttcg aacggttcat aacccgattt attgagcttc gcatcgctga aaaaataggc
     5581 gggcgcagcg ggctggtcat tggtgacgag tgaaacgaaa acttcacgcg ggatttcctg
     5641 cagcgcataa ttcttctttt tctgttcgcc gatcgtcgtc atcgtttcct tcccgatatt
     5701 ttttccgcag gcagaacctg cgccatgccc cgggtaaacg atcacatcat ccggcagcgt
     5761 ttttattttc tgaatggaat cgtacagttt cgctgcgagc gtttccgcgt cgaggtttcc
     5821 gctgagaaga tccggccggc cgacatctcc ggcaaataaa gtatcgcccg tgaacacggc
     5881 atgcggattt ttggcttcat catacagcag gaaacaggac gattcgaccg tatgacccgg
     5941 cgtatgcagg atctcgatct ccgttttccc gatccgcagg cgttcatggt ctttcgcgat
     6001 ataagcggga taggaaggtg tcgcatccgg accgaaaacg atcgccgctt ttgtcgctgc
     6061 cgccaggtcg agatgaccgg aaacgaaatc cgcgtggaaa tgcgtttcaa gaatgaactt
     6121 gatcgacgcg ccgcgcgaac gcgcaaactc gatgtagggt tccgggtcac gcagcggatc
     6181 gatgatgagc gcttcacggt cagactccac atagtaggca gcctgcgcga gacaattggt
     6241 gtaaaactgg tggatgtaca tggaatgaag ttttttgtgt gaattgtttt atgatccgat
     6301 cataggttgc cgtttgcgtg ccattctgcg aacgaggtcg ctgtctcatc gagacgaaga
     6361 ccggaaacgg ggaagttgtt ttcttctgca gcgagccttc gctgaaattc caggagcagc
     6421 atttcgcacg tgggcggaaa aggcaggtag ataactttcc cgcttaattc atttgcagga
     6481 aaatgttcgc ggtcggactg gcagagcacg agcgcatgat cgaaaacgtc gatgatgttt
     6541 tttcgcacga cgcggccgat atcggtaaag tcgatgacca tcccgttttt cggatccccg
     6601 ggtttgtcag ccggtttccc
//
LOCUS       DASTSF010000003         2450 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1781729,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000003 DASTSF010000000
VERSION     DASTSF010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2450
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1781729"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1339)
                     /locus_tag="VFU15_00045"
     CDS             complement(<1..1339)
                     /locus_tag="VFU15_00045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716186.1"
                     /translation="MRNFIVLLFSFSSVMLGAQTSMRWEQAYGGGGDDYGYSVKSCPG
                     QGYIVAGSTSSSGISDGWLIRTDSLGLVMWTKTYGGNNVDVLRSVKILPDSGYILAGF
                     SNSAGNGGYDGWALRTDKNGDTLWTKYIGTSDWDFFYDVAPTWDGGFVFAGGTYGRGN
                     GDEDMYFVKLDANGDTVWTKTWGGVKADEARSVIQTGDSLLASCGFTYSLGDSLGDSW
                     ILRMTGVGDTVWTRTINVPNFEDKAWGLSDVNLFTRFMVVGETSGTGDADGYFSGFDY
                     NGTLLYIDTTGAIGHDCFSSIVTRPNGTFAALGSSENLGSGSGDFFFFHDRNGWFETE
                     YGTTGIEQGYSIDITHDDGYIMCGYTVGFNLAQPNIFLIKTDTSAATSYVLLVKNPIV
                     SSGMGSVSIYPNPGNTYTSVFLNTSKNISDDLELRLYDVSGKLINTYSKSKWEI"
     gene            complement(1351..>2450)
                     /locus_tag="VFU15_00050"
     CDS             complement(1351..>2450)
                     /locus_tag="VFU15_00050"
                     /inference="COORDINATES: protein motif:HMM:NF040164.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5723 family protein"
                     /protein_id="HEU4716187.1"
                     /translation="DFLNTFYRGGYISHDKIDAGKNHLLPKNHVGIYTGMSAAYTWRN
                     KNDTVRWDFTVAFRDRQAIHGLFTDDAYRLVFDGNEPYAGTTLDLGGTHFTAIHWQQL
                     QLEAKYFYPDGRSEIAFGFSLMNGTSMSEVSIDRGTLFTPTTGTSLDLGLTGSYYASD
                     TASKKYFTNNGFGSCFNFRFSTFLGNPDGRYRHQLSFSVQDLGYMHWNSRSIVYTVDT
                     TMHYTGADVSGAIINHEQVTGFPSADSIFGSKKVAQVITFLPIGIRARYVLLTPTPFW
                     GGVEAKFWSNGDALPMVTIFAGWRNAKNNFNVVSGVSGGGYGKISVPLMLAWSPCRNF
                     RIMAGCDNVAGEIAPKKTHAQGLFTNLSFAF"
BASE COUNT          604 a          647 c          612 g          587 t
ORIGIN      
        1 taatttccca ttttgattta ctatatgtgt tgattaattt acctgatacg tcatacaatc
       61 taagttctaa gtcatcagaa atatttttcg aggtattcag aaatacactt gtgtaagtat
      121 tcccgggatt agggtatata ctgaccgaac ccatgccgga tgatactatc ggatttttga
      181 ccaaaagaac ataagatgtc gcagcagatg tgtctgtttt gatcaaaaaa atatttggct
      241 gggcaagatt aaatccgact gtatatccac acataatata tccatcatca tgcgtaatat
      301 caatgctgta gccctgctca ataccggttg tcccatattc tgtttcgaac catccatttc
      361 tgtcatgaaa gaaaaagaaa tcaccggaac cgcttccgag attttcagaa gagcccagag
      421 ctgcaaaagt tccattcggt ctcgttacta tgcttgaaaa acaatcatgt ccgattgctc
      481 cggtcgtatc aatatataat agggtgccat tgtagtcaaa cccgctgaaa tatccatctg
      541 catccccagt tcccgaagtt tcaccgacaa ccataaacct ggtgaagagg ttgacatctg
      601 aaagccccca ggctttatct tcaaaattag gaacattaat ggtcctcgtc cacaccgtat
      661 ctcccacacc ggtcatgcgc aaaatccagc tgtcgccgag cgaatctccg aggctatagg
      721 tgaatccgca ggaggcgagg agggaatcgc cggtctggat gacggaacgt gcttcatcgg
      781 cttttacgcc gccccaggtt ttcgtccata cggtgtcgcc gttcgcatcg agcttcacga
      841 aatacatgtc ctcatcgccg ttgccgcgcc cgtatgttcc gcctgcgaaa acaaatcccc
      901 cgtcccaggt cggtgccacg tcgtagaaaa aatcccagtc ggaagtgccg atgtattttg
      961 tccagagcgt gtcgccgttc ttatcggtgc gcagcgccca gccgtcgtaa ccgccattgc
     1021 ctgcggagtt ggaaaaaccg gcgaggatgt acccggaatc gggaagtatc ttcacgctgc
     1081 gcagcacgtc gacgttgttt ccgccgtatg ttttcgtcca catcacgaga ccgaggctgt
     1141 cggtgcggat gagccacccg tcgctgatgc cgctgctgct cgtagatccg gcaacgatgt
     1201 agccctggcc gggacaggat ttgacgctgt agccgtaatc gtccccgcct ccgccatacg
     1261 cctgttccca gcgcatggaa gtctgcgcgc caagcatcac cgaagaaaac gaaaacagga
     1321 ggacaataaa atttctcatg tccgtaattt ttaaaatgcg aatgaaagat tggtgaacaa
     1381 tccctgtgca tgcgtctttt tcggagcgat ctccccggcg acattatcgc agccggccat
     1441 gatgcggaaa ttccggcacg ggctccaggc cagcataagc gggacggaaa tttttccata
     1501 gcctccaccc gaaacgccgc tgacgacatt gaaattgttt ttcgcgttgc gccacccggc
     1561 gaagatcgtc accatcggaa gcgcatcgcc gttgctccag aatttcgctt cgacaccgcc
     1621 ccagaaaggg gtgggtgtaa gcagcacgta gcgcgcacga atgccgatcg ggaggaacgt
     1681 gatcacctgc gcgacttttt tcgatccgaa aatggaatcc gcggaaggaa agccggtcac
     1741 ctgttcgtgg ttgatgatcg ctcctgaaac atcggcgccc gtataatgca ttgtcgtgtc
     1801 caccgtgtaa acgatagacc tggaattcca gtgcatatac ccaaggtcct gcacggaaaa
     1861 cgaaagctgg tgccggtatc ttccatccgg gtttccaagg aaagtcgaga aacggaaatt
     1921 gaagcaggag ccgaaaccgt tgttggtgaa atattttttc gaggcggtat cgcttgcgta
     1981 atacgacccg gtaagaccaa gatcgagcga ggtaccggtg gtcggtgtga aaagtgtccc
     2041 gcgatcgatg gaaacttcgc tcatcgacgt tccgttcatc agcgaaaaac cgaatgcgat
     2101 ctccgatctt ccgtccgggt aaaaatattt cgcttcgagc tgcagctgct gccagtgaat
     2161 ggccgtgaaa tgcgtaccgc cgagatcgag cgttgttcct gcgtacggtt cgttgccgtc
     2221 gaatacaagc cggtaggcgt catcggtaaa cagcccgtgg atcgcctgcc ggtcgcggaa
     2281 agcgacggtg aagtcccagc gcactgtatc gtttttgttc cgccacgtgt acgcagccga
     2341 catcccggtg tagatcccga catgattttt cggaagcagg tgatttttcc cggcgtcgat
     2401 cttgtcatgc gaaatatacc cgccgcggta aaatgtgttc aggaaatcat
//
LOCUS       DASTSF010000004        13629 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_439810,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000004 DASTSF010000000
VERSION     DASTSF010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 13629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13629
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_439810"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..657
                     /locus_tag="VFU15_00055"
     CDS             <1..657
                     /locus_tag="VFU15_00055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716188.1"
                     /translation="PTLIYIYFALGVPKAHRAPFFGPRHAFVHKDKNGNDVTDTAYFS
                     IPAFACKTVNNIPFQSATLDHHMYVALFVRPDSIDALLPVLAQDVRLNRSDYKYARFA
                     FFYPGDSAGNAPASAPDFASDLKLGVDTAFTLFVSRAAFDSLHDNAYFVKDASRKNDP
                     WKTYSDAVIVDYRGRIRGYYDIRSASELKKLKEDVNFIYFRDESAETIENSTIEQNRK
                     "
     gene            654..1310
                     /locus_tag="VFU15_00060"
     CDS             654..1310
                     /locus_tag="VFU15_00060"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEU4716189.1"
                     /translation="MRTRIAIVAAAAFFVFSCKPKNNDAAGEITDVGGIQRLMIYYPL
                     DSTGPGGEKLYHTISPDTLTGQNGKPFLTASMKGNVVVTDFFFASCGGICPKMTSQLT
                     RVQAAIGKRNGFHIVSYSVDPERDSFPALQQYAERFSADTSMWTFVTGPKKRLYDLAR
                     YSYYLPVQPGNGDSEDFIHSDQVVLLDRQMRIRGYYNGTDSTAVDSLITDVKTLLEEK
                     "
     gene            1333..1869
                     /locus_tag="VFU15_00065"
     CDS             1333..1869
                     /locus_tag="VFU15_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013633641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF420 domain-containing protein"
                     /protein_id="HEU4716190.1"
                     /translation="MSEKAIFRMVMAVSVVVFVAVVILNKKILPVPAVIPHFVYHLPK
                     FHAFLNGTCSLILISSFLAIKNKRVTLHRRLNIAAFFISSLFLVSYITYHWLAPETSF
                     PTNNPFRPFYLFILITHIVLAGVVLPLILLSFYQGLKNNIVKHRKLVRWTFPIWLYVT
                     ITGVVVYLMICPWYNFPA"
     gene            complement(1814..2092)
                     /locus_tag="VFU15_00070"
     CDS             complement(1814..2092)
                     /locus_tag="VFU15_00070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716191.1"
                     /translation="MKKETLESRIKKADEYLVGLWKFRERGKRPVWCTTFLYQGYYYD
                     TPGKNSPEAALDALIEKMEKLKKKHPPEKKATPGNYTRGRSSGKQQRP"
     gene            2154..2390
                     /locus_tag="VFU15_00075"
     CDS             2154..2390
                     /locus_tag="VFU15_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011708121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716192.1"
                     /translation="MKRVTLLTLLLLLITVAAVAQCSMCRAVATSGTANGQNTAGGIN
                     SAILYMMVFPYALLFIFFRKKIFGFLKELRGLWR"
     gene            complement(2396..3268)
                     /locus_tag="VFU15_00080"
     CDS             complement(2396..3268)
                     /locus_tag="VFU15_00080"
                     /inference="COORDINATES: protein motif:HMM:NF025420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4249 domain-containing protein"
                     /protein_id="HEU4716193.1"
                     /translation="MRKLLFILPVLFTLSGCVPKPIDIDVPPAPEKLVVAAQVIPNHI
                     MVIGLTHSFSALDSGGNQDTLSNSFINRILVSHAIVTLDHPGGTDTLYQLTPGIYASV
                     NVLLSSYGSYTLHAKDPETGEEIKATTQLLPQIRFDTIIPSIVLAGNDSTVNIHYELT
                     DFPAEENYYVVSYYRKSQDTSAFDIYSYFQQGSNELNTFDLVSDADFDQDGKLSRDKQ
                     LPGIGINDTIAVTVANITKGYYEFLTAYKRSGSLFNQLSGEPIDFPTNVEGGYGYFAT
                     QFPDVHFYYMSNYH"
     gene            complement(3277..5565)
                     /locus_tag="VFU15_00085"
     CDS             complement(3277..5565)
                     /locus_tag="VFU15_00085"
                     /inference="COORDINATES: protein motif:HMM:NF025092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEU4716194.1"
                     /translation="MKLRFTLLLLILTFLAKAQGSFTISGYVSDSASGEMLIGASVYV
                     EGTTKGTAANVYGFYSLTLAAGDYTIICNYIGYHAQKISVSLNADQKTDFKLSNSTAS
                     VRKEVVVTADRNRDEVRSTQTSMITIPVDKVRTIPTIGGETDIIKVVQLMPGVKRGGE
                     GQNTMFVRGGSGDDNLILLDEATVYNVSHLFGFFSVFNNDALKDVTLIKGGFPAQYGG
                     RLSSVMDVRMKDGDMEKFHAAGGIGLLSSHLTIEGPLKKDTSSFLISARRSYIDKVFK
                     LAYHGQNVLPYFFYDANVKWNYILSSRDRIFVSGYFGDDVLSAKEENDSSFFDGGFKL
                     GNFTTTARWNHTYNSKLFSNISFIHTRFRYDVEASIPGNSFLTRSRIQDFEGVADYSY
                     FRKPGNTVRFGMAYTTHVFRPNVVSTSGEISDYLRSREGTRIFTNEIAFYGNYECDLD
                     SNFRVSGGLRLATLVSPGKTYFSPEPRLSGTYILSDNQSFKLSYSRMTQFLHLVSSSS
                     IALPTDLWYPVTKNVKPIYADQIAAGYDLNVPKIKTLFTFEAYYKWMQNLIEYREGAV
                     LILNDNYEQELVSGNGHAYGFEFFAQKQEGKFTGWVGYTISWSIRQFDELNHGNPYFA
                     KYDRRHDISVVMMYEFTKRFSVSAVWVFSTGQRFTPETGNFLMPDATLTSVNVLPIYT
                     DKNAIVLPPAHRLDINFVLKSRLNRKWMKWTGEWQFGAYNVYNRAQPYKIKIVPTDNG
                     TYKYEAVGLFGFIPSIAYNFRF"
     gene            complement(5666..6964)
                     /locus_tag="VFU15_00090"
     CDS             complement(5666..6964)
                     /locus_tag="VFU15_00090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716195.1"
                     /translation="MKKALFSVAFISSALCGQNFVPNPGFETYKELPCEPMQFNMSDY
                     VPDWQSAAGGTPDVKYDYPGINKDCYANCFSTNPESYGSEKPHSGHGMGMIMTDALGG
                     SYREYLAATLTQPLVKGVTYYVEMYVSLGDNCTQQTNNVGIAFFTGTFSRASGNIIIT
                     TPAVNESKVLSKAEGWTKISGTFTADDNYTYFAIGNFLTRPQTKIEKRTAKDSTHNYH
                     TDITAYYIDDIVVRPNECSLSIGGDTLVLVGTNARLIASGGTDYTWIENGKPRDTLSH
                     TSVLDVKMDKARVFVVSDESCRRTVTVGVLKPGDFAKKDLNGRKVKKGREVNVQNDVI
                     SVIIYDKDEVDGDSISLWYGDSCLVQHYALTKKKKEFKVKIDKDHPKQLILYAENEGS
                     KPPNTAAFIIKDGRKSSNLILSSDLKSCDAVMLVHKDAPK"
     gene            complement(7013..8308)
                     /locus_tag="VFU15_00095"
     CDS             complement(7013..8308)
                     /locus_tag="VFU15_00095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716196.1"
                     /translation="MRKTLTIILFLPLMIGAQNLVPNSGFEEYHRVPCDCMQGDLSEY
                     IKNWQSPAAGTPDIITDEADNDCYASCYSKNNQSYGTQAPHGGHGMGMIMTTALSGSY
                     REYLGVQLKAPLIPGKKYYAEMYVSLGDKSGLATNNLGMCFFDGDFKKSTSNIITATP
                     QVNSKDVVDNYVGWVKISGTFVADAPYRVLVIGNFLPMDETDTKKRSNTGSGAYSYMR
                     KDIAAYFVDDILVRQGGSLEVHGDTLVNQGAMDLLTATGSTRYAWADTTHPATILSNT
                     DQLNMPMNSRRTFYVYGDDDTAMITVNVRIPKPDIVDSLDDRKVKKGRTITVTNDKVT
                     ITVYDKDELDGDSISLWYGDSCLVQHYALTKKKKEFVIHVDKNHPRQLILFAENEGRK
                     PPNTAAFIITDGKHSDNLVLSSDMKSCDSVMLVYKEPPK"
     regulatory      8597..8764
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            8845..9603
                     /locus_tag="VFU15_00100"
     CDS             8845..9603
                     /locus_tag="VFU15_00100"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU4716197.1"
                     /translation="MKTTERFSDRVANYVKYRPGYPAEVLNHLREAGVLRTDSVIADI
                     GSGTGISARLFLENDFEVFGVEPNREMREAGESFLAAYKRFHSVDGTAEQTTLADRSV
                     DVVVAGQAFHWFDAEKAKTEFRRILRPGGYVVLAWNDRRTDSTDFLKAYEDLLKMFGT
                     DYAKVNHRNIDEKKFDEFWGTGKWHSFSVYNFQDFDFAGLKGRLLSSSYVPAENHADS
                     KLMLDVLKKIFLRYQENGKVRFEYDTRVYYGKTD"
     gene            9618..13286
                     /gene="metH"
                     /locus_tag="VFU15_00105"
     CDS             9618..13286
                     /gene="metH"
                     /locus_tag="VFU15_00105"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015030468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HEU4716198.1"
                     /translation="MRDIKKELEKRVLILDGAMGTMIQRYKLSEADYRGTRFAGWPHD
                     LKGNNDLLVLTQPQIILEIHKQYLEAGADIIETNTFNAQKISLADYHMQSLAYEMNHE
                     AAKIARAAADEFTAKDPSRPRFVAGAMGPTNRTASLSPDVNDPGYRAVTFDDLVDAYS
                     EQAKGLLDGGVDVFLIETIFDTLNAKAALFAVQSLQETAGRKIPVMISGTITDASGRT
                     LSGQTTEAFLNSVSHVDLLSVGLNCALGAAQMRPFLEEIAEKAPFYVSCYPNAGLPNQ
                     FGEYDQTPESMGEQVSDFIRSGFINIIGGCCGTTPAHIRRIAEIAKGNRPRNVPYREP
                     YLRLSGLEPVTLRPESNFMNVGERTNVTGSRKFAKLITESKYDDALAIARDQVEGGAQ
                     VIDINMDEGMLDSEAAMVRFLNLIAAEPDIARVPIMLDSSKFHVIESGLKCVQGKAVV
                     NSISMKEGEAEFKRQAALVKKYGAAVIVMAFDENGQADTLQRRIDICKRAYDILVNEV
                     KFPPQDIIFDPNIFPVATGMEEHRRNAMDFFLATKWIKENLPHAKVSGGVSNVSFSFR
                     GNDKVREAIHSAFLFHGIKHGMDMGIVNPGQLQVYDEIPKELLGLVEDVLLDRNDDAT
                     ERLIAYAEKIKGDGGTKKQEKDEEWRKGTVGERLTHALVKGIVDHIEQDVEEARKQFA
                     KALQVIEGPLMDGMNVVGDLFGAGKMFLPQVVKSARVMKKAVAYLTPYLEAEKVAGSG
                     KSAGKVVLATVKGDVHDIGKNIVGVVLACNNYEIVDLGVMVSADRILEAAQKEKADVI
                     GLSGLITPSLDEMVHVAKEMERRGFTTPLLIGGATTSKVHTAVKIAPNYSRPVVHVND
                     ASRSVPVVSSLISDELRGNFMKEVEKDYERLREQNSRAHGANKFISLAEARANKFSTD
                     WDALHFVKPSFTGTKAFEDYPLEELVPYIDWTPFFHSWEMKGSYPKIFNDPERGTEAK
                     KLFDDAQKMLKLIVREKWITAKAVIGFWPANTVNDDEMEIFANEKRADVIAKFEAIRQ
                     QTKKPGGQANVALSDFLAPAGAEDYLGGFAVCAGYGVDEKVKQFEKDHDDYSAIMLKS
                     LADRLAEAFAERMHERVRKEFWGYAKDEHFTNEDLIKEQYRGIRPAPGYPAQPDHTEK
                     IALFRLLDAEKKIGLQLTENLAMYPTAAVSGLYFSHPKAHYFGVGKITKEQVEDYAKR
                     KGFSVEEAEKWLGTVLGY"
BASE COUNT         3151 a         3471 c         3807 g         3200 t
ORIGIN      
        1 ccgaccctta tttatattta tttcgcgctc ggcgttccca aggcacaccg ggcgcctttt
       61 tttgggccga ggcatgcctt cgtgcacaaa gacaagaacg gaaatgatgt aacggacacg
      121 gcatatttca gcattcccgc atttgcctgc aaaaccgtca acaatattcc tttccagagc
      181 gcaacgctgg accatcacat gtatgtggcg ttgtttgtcc ggcccgattc catagacgca
      241 ctccttcctg ttcttgcgca ggatgtccgg ctcaaccggt cggattacaa atacgcgcgt
      301 tttgcatttt tctatcccgg cgattcggcg ggaaatgcgc cggcctccgc tcccgatttt
      361 gcttccgatc tgaaattggg cgtcgatacg gctttcacgc tcttcgtttc gcgtgcggca
      421 ttcgattcgt tgcacgacaa tgcttatttc gtgaaagacg cttcccgcaa aaacgatccg
      481 tggaaaactt acagcgacgc cgtcatcgtg gattaccggg gaaggatccg cgggtattac
      541 gatatccgtt ccgcttcgga gctgaagaag ctgaaagagg acgtgaattt tatttatttc
      601 cgcgacgaat cggcggagac gatcgaaaat tccaccatag agcagaaccg gaaatgagaa
      661 cacgaattgc catcgtcgct gctgctgcgt tttttgtttt ttcctgcaaa ccgaaaaaca
      721 atgacgccgc cggcgaaatt acggacgtgg gcgggataca gcggctgatg atctattacc
      781 cgctcgattc aaccgggccg ggtggagaaa agctgtatca cacgatcagt cccgacacgc
      841 tgaccgggca gaacggcaaa ccgtttctca cggcgtcgat gaaaggaaat gtcgtggtaa
      901 cggatttttt cttcgccagc tgcggcggta tctgcccgaa gatgacgagc cagcttacgc
      961 gcgtgcaggc ggcgatcggg aagaggaacg gctttcatat tgtttcctat tcggtggatc
     1021 ccgaacgcga ttcttttcct gcgctgcagc aatacgccga acggttcagc gccgacacat
     1081 cgatgtggac gttcgtcacc ggtccgaaaa agagacttta cgatcttgcg cggtacagct
     1141 attacctgcc cgttcagccc ggtaacggcg actcagaaga tttcatccac agcgaccagg
     1201 tggtgttgct cgaccggcaa atgcgcatcc gcgggtatta caacggaacc gacagcacgg
     1261 ccgtcgacag cctgatcacg gatgtgaaaa cattgctgga ggaaaaataa ttccagtaaa
     1321 aacagacgca acatgtcaga gaaagcgatt ttcagaatgg tgatggccgt ttccgtcgtg
     1381 gtgttcgtgg cggtcgtcat cctgaataaa aagatactcc cggttcccgc cgtgatcccg
     1441 catttcgttt accatctccc gaaattccat gcgtttctca acggaacgtg ctcgctcatt
     1501 ctgatcagtt cttttttagc tataaaaaac aagcgcgtca cgctgcacag gcggctgaac
     1561 attgcggcgt ttttcatctc gtcgctgttc ctcgtttcct acatcaccta tcactggctg
     1621 gcgccggaga cctccttccc gacgaacaac ccgttccgcc cgttttacct gttcatcctc
     1681 attactcaca tcgtgctggc gggagtggtt ctgccgctca tcctgctttc gttctaccag
     1741 ggactcaaga acaatattgt caagcaccgt aagctggtga ggtggacttt cccgatctgg
     1801 ctgtacgtga cgattacggg cgttgttgtt tacctgatga tctgcccctg gtataatttc
     1861 ccggcgtagc ttttttttcc ggcgggtgtt ttttcttcag cttctccatt ttttcgatca
     1921 gcgcgtcgag tgcggcttca ggcgaatttt ttcccggcgt atcgtaataa taaccctggt
     1981 aaagaaaggt ggtgcaccac accgggcgtt tgccacgttc gcggaatttc cagagaccga
     2041 caaggtattc gtccgctttc ttaatgcgcg attcgagcgt ttcttttttc ataggcggca
     2101 aaaatacgaa tagggtcgcg gcggctgatt aaaaccgtaa atttgctgtt gctatgaaac
     2161 gggttaccct gcttactttg cttctcctgc tgatcactgt cgcggcagtt gcgcaatgct
     2221 ccatgtgcag ggccgttgcc accagcggca ccgccaacgg ccagaataca gccggcggca
     2281 tcaacagcgc catcctctac atgatggttt tcccttatgc gctgctgttt attttcttcc
     2341 gcaaaaagat cttcgggttc ctgaaagagc tgcgcgggct gtggcgctga aaagatcaat
     2401 gataattgct catgtaataa aaatgcacgt cgggaaactg cgtggcgaaa tacccgtaac
     2461 cgccttccac gttcgtgggg aaatcgatcg gttcgcccga gagctggttg aaaagactgc
     2521 ctgaacgttt gtacgccgtc aggaattcat aatacccttt cgtgatgttc gccaccgtaa
     2581 cagcgatcgt atcgttgatg ccgatgccgg gcagctgctt gtcgcgcgaa agcttcccgt
     2641 cctggtcgaa atccgcgtcg gaaacaagat cgaacgtgtt cagctcgttg cttccctgct
     2701 ggaaatagct gtagatgtcg aacgcactcg tgtcctggct tttgcggtaa taggaaacga
     2761 cataataatt ttcttccgcg ggaaaatccg tcagctcgta atgtatgttg acggtggaat
     2821 cattccctgc gaggacgatc gaaggaatga tcgtgtcgaa ccggatctgc ggaagcagct
     2881 gcgtcgtcgc ttttatttct tcgccggtct ccgggtcttt cgcatgcagc gtatacgagc
     2941 cgtagctgct cagcaggacg tttacgcttg cataaattcc cggcgtaagc tggtaaagcg
     3001 tatctgttcc gcccggatga tcgagcgtga cgatcgcatg gctgacgaga atacggttga
     3061 tgaacgaatt cgacagcgta tcctggttgc cgcccgaatc gagcgcactg aagctgtgtg
     3121 tcagcccgat gaccatgatg tggttcggga tcacctgcgc ggcgaccacc agcttttccg
     3181 gtgcaggcgg aacgtcgatg tcgatcggtt tcggaacgca gccggaaagc gtgaacagga
     3241 cgggcagtat gaacaataat ttcctcatga ttgttttcag aacctgaaat tgtaagcgat
     3301 ggaaggaatg aaaccgaaaa gcccgacagc ttcgtattta tacgtgccat tatccgtggg
     3361 cacgatcttg atcttgtacg gctgcgcccg gttgtacaca ttgtatgcgc cgaactgcca
     3421 ttcgcccgtc cacttcatcc atttgcggtt gaggcggctt ttcagcacga agttgatgtc
     3481 aagccggtgc gcaggcggaa gcacgatggc gttcttgtcg gtgtagatgg gaagaacatt
     3541 cacgctcgtc agcgtggcgt cgggcatgag gaaattcccg gtttcaggcg tgaagcgttg
     3601 tcccgtgctg aacacccaca cggcagagac ggaaaagcgt ttcgtgaatt catacatcat
     3661 cacgacggaa atatcatgac gccggtcgta tttcgcgaaa tacgggttgc cgtggttgag
     3721 ctcatcgaac tgccgtatgg accaggagat cgtatagcca acccagccgg tgaattttcc
     3781 ttcctgcttc tgcgcaaaga attcgaaacc gtaggcgtga ccattcccgc tcacgagttc
     3841 ctgctcgtaa ttgtcgttga gtatcaggac agcgccctcg cggtattcga tcaggttctg
     3901 catccatttg taatacgctt cgaacgtaaa gagcgttttt attttcggga cattgaggtc
     3961 ataacctgcc gcgatctgat ccgcgtagat cggcttcacg tttttcgtga ccgggtacca
     4021 gagatcagtc ggcagcgcga tggaagagct ggaaaccagg tggaggaatt gggtcatccg
     4081 cgaatacgaa agcttgaacg actggttgtc gctgaggatg tacgttccgc tgaggcgcgg
     4141 ttcgggactg aaatacgttt tccccggtga aacgagcgtg gcgaggcgca acccgccgct
     4201 gacgcggaaa ttgctgtcga ggtcgcattc gtaatttccg tagaacgcga tctcgttcgt
     4261 gaaaatgcgc gtgccttcgc gcgagcgcag gtaatcggaa atctctcctg acgtgctgac
     4321 gacgttcggg cggaacacat gggtcgtata cgccattccg aaacgcaccg tgttgcccgg
     4381 cttgcggaaa tagctgtagt cggcgacgcc ttcgaaatcc tggatgcgtg aacgcgtgag
     4441 aaaagaattg ccgggaatgc tcgcttccac atcatagcgg aaacgcgtgt gaataaacga
     4501 aatgttcgag aacagctttg aattgtaggt gtggttccat cgtgcggtgg tggtgaaatt
     4561 tccgagcttg aaaccgccgt cgaagaagga gctgtcattc tcttccttcg cgctcagcac
     4621 atcatcgccg aagtagccgc tgacgaagat ccggtcgcgg gaagaaagaa tgtaattcca
     4681 tttcacattc gcatcgtaga aaaaataagg aagcacgttc tgcccgtggt acgccagctt
     4741 gaacactttg tcgatgtatg atctccgagc ggaaatgaga aaagacgacg tgtctttttt
     4801 cagcggacct tctatcgtga ggtgcgaaga gagcagcccg atcccgccgg ccgcgtgaaa
     4861 cttttccatg tcgccgtcct tcatgcgcac gtccatcacc gaagaaaggc gcccgccgta
     4921 ctgcgcggga aatccgcctt tgatgagcgt cacgtctttc agcgcgtcgt tgttgaacac
     4981 ggaaaaaaat ccgaagagat gcgacacgtt gtataccgtc gcttcgtcga gcaggatgag
     5041 attatcgtca ccggagccgc cgcgcacgaa catcgtgttc tgtccttctc cgccgcgttt
     5101 cacgccgggc atgagctgca cgactttgat gatgtcggtt tccccgccga tggtgggaat
     5161 ggtgcgcacc ttgtcgacgg gaatggtgat catgctggtc tgcgtcgatc gcacttcatc
     5221 gcggttgcgg tcggcggtaa cgacgacttc cttgcgtacc gatgcggttg agtttgaaag
     5281 cttgaaatcc gttttctgat cggcgttaag cgaaacactg atcttctgcg cgtggtaacc
     5341 gatgtaattg cagatgatcg tgtaatcgcc ggcggcaagc gtgagcgaat agaatccgta
     5401 cacattggct gcggttcctt tcgtggttcc ttccacgtaa acggaagcgc cgatgagcat
     5461 ttcaccgctc gcggaatcgg aaacgtaacc gctgatcgta aatgagccct gcgcttttgc
     5521 gaggaaagtc aggatcagca ggagtaaagt gaagcggagt ttcatgcagc cgacgtaaga
     5581 cgggaatgac cgtcacgaag ttacttgctg caagttaaaa caatgcggga aaagaatgaa
     5641 ccgggaaacg aaggcccgct cccgcttact tcggcgcatc cttgtgcacg agcatcaccg
     5701 catcgcagga tttcagatcg gagctgagga tgaggttgga gcttttcctg ccatccttga
     5761 tgatgaacgc ggcggtgttc ggcggtttgc tgccttcatt ttccgcgtaa agaatgagct
     5821 gcttcgggtg gtccttgtcg attttaacct tgaactcttt tttctttttt gtcaacgcgt
     5881 aatgctgcac gagacaggaa tcgccgtacc agagtgaaat gctgtcgccg tccacttcgt
     5941 ccttatcgta aatgatcacg gagatgacat cgttctgcac gttcacctct cttccttttt
     6001 ttacttttct cccgttgaga tcttttttgg cgaaatctcc cggtttcagc acaccgacag
     6061 taaccgtacg ccggcagctt tcatcggaaa cgacgaatac ccgcgctttg tccatcttaa
     6121 cgtcgagcac gctggtgtgc gaaagcgtat ctctcggttt cccgttttcg atccaggtgt
     6181 aatctgttcc gccggaagcg atcagtcttg cattggttcc gaccagcacg agcgtgtcgc
     6241 cgccaatgga aagcgagcat tcattcgggc ggacaacgat atcgtcaatg taataagcgg
     6301 taatgtccgt gtgataatta tgcgtggaat cctttgcagt tcttttctcg atcttagttt
     6361 gcggtcgcgt gaggaaattc cctatcgcga aatacgtgta gttatcgtct gcggtgaatg
     6421 tcccgctgat cttcgtccag ccctcggctt tcgacaggac cttcgactca ttgaccgccg
     6481 gcgttgtgat gatgatattc cccgatgcac gggaaaatgt accggtaaaa aaagcgatgc
     6541 cgacgttatt tgtctgctgc gtgcagttgt cgccgaggga aacatacatt tccacgtaat
     6601 aggtgacgcc tttaaccaat ggctgcgtga gcgtggcggc gaggtattcc ctgtaggagc
     6661 cgcccagcgc atcggtcatg atcatgccca tgccgtgacc gctgtgcggt ttttcacttc
     6721 cgtagctttc cgggttggtg gaaaagcaat tggcgtagca atctttattt attccgggat
     6781 aatcatactt gacgtcgggt gttccgccgg ctgcgctttg ccagtcggga acataatcac
     6841 tcatgttgaa ctgcatgggc tcgcagggaa gttccttgta ggtttcgaaa cccgggttgg
     6901 gaacgaaatt ctgtccgcac agggcggaag aaataaatgc aacggagaac agggcttttt
     6961 tcatgggaga atcgttgcta tctataaccg cggatgtggc gaaaagtcac atttatttcg
     7021 gcggttcttt gtagaccagc atcacggagt cgcatgattt catgtccgag ctgagcacga
     7081 ggttgtcgct gtgcttaccg tccgtgataa taaatgcggc ggtattcggc ggcttgcggc
     7141 cttcattctc ggcgaacagg atcagctgcc tgggatggtt cttgtcgacg tgaatgacga
     7201 attccttttt ctttttcgtc agcgcataat gctgcacgag acaggaatcg ccataccaca
     7261 gtgaaatgct gtcgccgtcg agctcgtcct tgtcgtaaac ggtgatcgtc actttgtcat
     7321 tggtgaccgt gatcgtcctt ccctttttca ccttgcggtc gtcgaggctg tccactatat
     7381 cgggcttggg aatgcgcaca ttcaccgtga tcatcgctgt atcgtcatcg ccgtaaacgt
     7441 agaacgtacg ccggctgttc atcggcatgt tgagctggtc cgtattcgaa agaatggttg
     7501 cgggatgcgt tgtgtccgcc catgcgtacc tggtgctgcc tgttgccgtg agcaaatcca
     7561 tcgcgccctg gttgacgagc gtatcgccgt gcacttcgag gctgccgccc tggcgaacga
     7621 gaatatcgtc gacgaagtag gcggcgatgt ctttccgcat gtagctgtaa gctccgctgc
     7681 ctgtgttgga acgctttttc gtatccgttt catccatcgg caggaagttc ccgatcacca
     7741 gcacgcggta aggtgcgtcg gctacgaacg ttccgctgat cttcacccag cccacgtaat
     7801 tatcgacgac gtctttggaa ttcacctgcg gtgtggctgt gatgatgttc gacgtcgatt
     7861 ttttgaaatc cccgtcgaaa aagcacatgc cgagattgtt ggtggccagg cccgatttat
     7921 caccgaggga aacatacatt tcggcatagt attttttccc gggaatgagc ggcgctttca
     7981 gctgcacgcc gaggtattcg cggtaggaac cgctgagggc ggtggtcatg atcatgccca
     8041 tgccgtgccc gccgtgcggc gcctgcgttc cgtagctctg gttgtttttc gaatagcagc
     8101 tggcgtagca gtcgttgtcg gcttcgtccg tgatgatgtc gggcgtacct gcagcgggac
     8161 tttgccagtt cttgatatat tcggaaagat cgccctgcat gcagtcgcag ggaacccggt
     8221 gatattcttc aaatccggaa ttggggacga ggttctgggc cccgatcatc agtgggagaa
     8281 acaagatgat ggtgagcgtt tttctcattg aaaagcgaag attgacagta cagagattaa
     8341 aggacgcggt actttccata aatcatcagc ctcggtttgg ggttaagaca cgatggccgc
     8401 ttcctttgta actaagttag tgtttaaaag taacggcaca gtttaaaatt cataaccggc
     8461 cggaatttct ttacgattca acgtaaagat cgcaccggga attgtgcccg ccaagcggga
     8521 agcccgcctc ttaccattat ttcacatctt aattttcagg ccgtttttcc tatctttgcc
     8581 ccggttcttt caagcaaact tatccggaaa ggcagaggga tagcgcccga tgaagccttg
     8641 gcaacccttg ttagaaggga gaacggagca gagaacgcgg agtcattctc cattctccag
     8701 cttccgcact ctgttctcga agaaggtgcc aactcgctct ccgcattgcg gggaaagata
     8761 agtcagaagt ccagcctctt gttcaactgc gcacccttct gactcttcgg aagggttttg
     8821 tttttcggga ggaccgccgg acagatgaaa acaacagaac gtttcagcga ccgtgttgcc
     8881 aattatgtga agtaccgtcc aggttatcct gcggaggtgc tgaatcattt gagggaggca
     8941 ggcgtgctgc gcacggattc cgtgatcgcg gatatcggct cgggaacggg aatttccgcg
     9001 cgactttttc ttgaaaatga tttcgaggtc ttcggtgtgg agccaaaccg ggaaatgcgt
     9061 gaagcaggag aatcatttct cgctgcgtac aaacgtttcc acagcgtgga cggaacagcc
     9121 gagcagacga cgcttgcgga ccggtccgtc gatgtcgtag tcgccgggca ggctttccac
     9181 tggttcgatg cggaaaaagc gaagacggaa ttcaggcgaa ttcttcgtcc cggcggttac
     9241 gtggttcttg cctggaatga ccggcggacg gattcgacgg atttcctgaa ggcgtacgaa
     9301 gatctgctga agatgttcgg cacggattac gcgaaggtga atcaccggaa catcgatgaa
     9361 aagaaattcg acgaattctg gggaacgggg aaatggcatt ccttttccgt ttacaatttc
     9421 caggattttg attttgcggg actgaaagga agactgcttt catcctcgta tgttcctgcg
     9481 gaaaatcacg cggacagcaa gctcatgctc gacgtgctga agaaaatatt cctgcgttac
     9541 caggaaaacg ggaaagtgag attcgaatac gacacacgcg tttattacgg caaaaccgac
     9601 tgaagcgaaa ggcatttatg cgagacataa aaaaagaact ggaaaaaaga gtgctcatcc
     9661 tcgacggtgc aatgggcaca atgatacagc ggtataaact ttccgaagcg gattaccgcg
     9721 ggacgcgttt tgccggatgg ccgcatgatc tcaagggaaa caacgacctg ctcgtgctca
     9781 cccagccgca gatcattctt gaaatccata agcaatacct cgaggccggc gccgacatca
     9841 ttgagacgaa cacgttcaac gcgcagaaaa tttccctcgc cgattaccac atgcagtcgc
     9901 ttgcgtacga aatgaaccac gaagcagcga agatcgcacg tgccgccgcg gatgaattca
     9961 ccgcgaaaga tccgtcacgt ccgcgcttcg ttgccggtgc gatgggaccg accaaccgta
    10021 ccgcttcgct ttctcccgac gtgaacgatc cgggttaccg cgccgttact ttcgatgatc
    10081 tcgtggacgc gtacagcgag caggcaaaag gtcttctcga cggtggcgtg gatgttttcc
    10141 tgatcgagac cattttcgat acgctgaacg caaaggcggc cttgttcgcc gtgcagagcc
    10201 tgcaggaaac cgccggacgg aaaatcccgg tcatgatctc cggcaccatc accgacgcaa
    10261 gcggaagaac actttccggt cagaccaccg aagcgttcct caattccgtt tcgcatgtcg
    10321 acctgctcag cgtcgggctg aactgtgcgc tcggtgctgc gcagatgcgc ccgttcctcg
    10381 aggagatcgc ggagaaagca ccgttctacg tgagctgtta cccgaacgca ggtttgccca
    10441 accagttcgg tgaatacgac cagacgccgg aatcgatggg cgagcaggtg agcgatttca
    10501 tccgcagcgg attcatcaac atcatcgggg gatgctgcgg aacgacacct gcgcatatcc
    10561 gccgcatcgc ggaaatagca aaaggaaacc gtccgcgcaa cgtaccgtac cgtgagcctt
    10621 acctgcgtct cagcgggctc gagccggtta cgctgcggcc cgaaagcaac ttcatgaacg
    10681 tcggtgagcg cacgaacgtc acgggctcgc gcaaattcgc gaagctcatt accgaaagca
    10741 agtacgacga tgcgctggcg atcgcacgcg accaggtgga aggcggcgcg caggtgatcg
    10801 acatcaatat ggacgaaggc atgctcgatt cggaagcggc catggtccgt ttcctgaatc
    10861 tcattgctgc ggaaccggat attgcgcgcg tgcccatcat gctcgactcg tcgaaattcc
    10921 acgtcatcga atcgggactg aaatgcgtgc agggaaaagc ggtcgtgaat tccatctcga
    10981 tgaaagaagg cgaggcggaa ttcaaacgcc aggctgcgct ggtaaaaaaa tacggggctg
    11041 ccgtcatcgt gatggcgttt gacgagaacg ggcaggcgga tacgctgcag cggaggatcg
    11101 acatttgtaa gcgcgcttat gatatcctgg tgaacgaagt aaaattcccg ccgcaggaca
    11161 tcattttcga tccgaatatt ttccctgtcg ccacgggaat ggaagagcat cgccgcaacg
    11221 cgatggattt tttcctggct acgaaatgga tcaaggaaaa tctcccgcac gcgaaagtca
    11281 gcggcggcgt gagcaacgtt tcattttcat tccgcgggaa cgacaaggtg cgcgaagcga
    11341 tccattccgc gtttcttttt cacggcataa agcacggcat ggacatgggt atcgtgaatc
    11401 ccggccagct gcaggtgtat gatgaaattc cgaaagaatt gctcgggctc gtggaggacg
    11461 tgctgctcga ccgcaacgac gacgctaccg agagactcat tgcgtacgca gaaaaaataa
    11521 aaggtgacgg cgggacgaag aaacaggaaa aagacgaaga atggagaaag ggaaccgtcg
    11581 gtgagcggtt gacgcatgcg cttgtgaaag gcatcgtcga tcatatcgag caggatgtgg
    11641 aagaagcgcg gaagcagttc gcgaaagcgc tgcaggtgat cgaaggtccg ctgatggacg
    11701 gcatgaacgt cgtcggcgat cttttcggtg cagggaaaat gttcctgccg caggtggtga
    11761 aaagtgcacg cgtgatgaaa aaggcggtcg cgtatctcac accatacctt gaagcggaga
    11821 aagtcgccgg aagcggcaaa tcggccggca aggtcgtgct cgccacggtg aagggcgacg
    11881 tgcatgatat cggcaagaac atcgtcggcg tggtgctggc ctgcaacaat tacgagatcg
    11941 tcgatctcgg cgtgatggtt tccgcggacc ggatcctcga agccgcgcag aaggaaaaag
    12001 cggacgtgat cggtctcagc ggactgatca cgccatcgct cgacgagatg gtgcatgtcg
    12061 cgaaagagat ggagcgccgc gggtttacca cgccgctgct catcggcggt gcgacaacat
    12121 cgaaagtgca taccgcggtg aagatcgcac cgaattattc gcgacctgtc gtgcatgtga
    12181 acgacgcctc gcgcagcgtg cccgtggtga gcagccttat ctcggatgag ctgcgcggca
    12241 atttcatgaa ggaagtcgaa aaagattatg aacgcctgcg cgagcagaac agccgggcgc
    12301 acggggcgaa taaattcatt tcactggctg aagcgcgcgc caataaattt tccaccgact
    12361 gggatgcgct gcatttcgtg aagccgtctt ttaccgggac aaaggcattc gaagattacc
    12421 cgctcgaaga gctggttccg tatatcgact ggacgccgtt cttccacagc tgggagatga
    12481 aaggaagcta cccgaaaata ttcaacgatc cggaacgcgg aacggaagcg aaaaaactgt
    12541 tcgatgatgc gcagaaaatg ctgaagctta tcgtgcggga aaaatggatc actgcaaaag
    12601 ccgtgatcgg gttctggccg gcgaatacgg tgaacgacga cgagatggaa atctttgcga
    12661 acgaaaagcg tgcggatgtc atcgcaaagt tcgaggccat ccgccagcag acaaaaaaac
    12721 cgggaggcca ggcgaatgtg gcgctttccg atttccttgc gcccgccgga gcggaggatt
    12781 acctcggcgg attcgccgtg tgcgccggtt acggtgtgga tgagaaagtg aaacaattcg
    12841 aaaaagacca cgacgattac agtgcgatca tgctgaagtc gctcgccgat cgtctcgccg
    12901 aagcgtttgc ggaacgcatg cacgaacgcg tgcgcaaaga attctggggg tacgcgaaag
    12961 acgagcattt taccaatgaa gatcttatca aagagcagta ccgcggcatt cgcccggcgc
    13021 ccggttatcc tgcgcagccc gatcacacgg agaaaatcgc gctgttccgc ctgctcgatg
    13081 cagaaaagaa aataggactt cagctgacgg aaaatcttgc catgtatccc acagcagccg
    13141 taagcggcct gtatttttcg catccgaaag cgcattactt cggtgtggga aagatcacga
    13201 aagagcaggt ggaagattac gcgaaacgga aaggattttc cgtggaggaa gcggagaaat
    13261 ggctggggac agtgttgggg tattaatacc cggtattcaa tttatgcagg cgagtacctg
    13321 caggtaaaca tccgccgcgg cgcgatcgcg tatgaagtaa atttcaattc agtcggcgac
    13381 tggaagcaca gcagtgacaa tgcgacgcaa ctataccggt tcctggccgg gaataagttg
    13441 tatgaggaat gatgggcgat tcctgttcgc ggaggtcttt tgaacgtgga aaatgcaaag
    13501 tacctttgcg ccaaatcaac ccgaaatgaa aaagatcatt cctgtagctg ttgtgttcgc
    13561 gtttgtgttt gcgtcgtgca agaaatacga tgacggtcct tacctgagcc tgcgctcaaa
    13621 aaaggagcg
//
LOCUS       DASTSF010000005         2643 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1778539,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000005 DASTSF010000000
VERSION     DASTSF010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2643
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1778539"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(39..1892)
                     /locus_tag="VFU15_00110"
     CDS             complement(39..1892)
                     /locus_tag="VFU15_00110"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014165638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEU4716199.1"
                     /translation="MAEKHNYTEDSIRSLDWKDHIRMRPGMYIGKLGDGSSTDDGIYI
                     LVKEVLDNSVDEFVMGHGKRIEVSVKDGIVTVRDYGRGIPLGKVIDCVSKINTGAKYD
                     SEAFKKSVGLNGVGTKAVNALSSWFRVQSIREGKTKVAEFKRGNLEKDERVKACDEAE
                     GTIISFKPDDKVFLNYHFINEYVEKMLWNYAYLNTGLTLWYNGNMIKSDNGLLDLLQK
                     NLSGPIIYPIIHLRGNDIEVAMTHSDQQYVEEYFSFVNGQHTTQGGTHQGAFREAVVR
                     VIRDFLKKEYEANDIRTSIVAAISVKVQEPVFESQTKTKLGSQDVGPKGPSMRSFVYD
                     FLKVELDNYLHKFPETADALHRKILQSERERKELSGIQKLARERAKKANLHNKKLRDC
                     RVHLTDLKDERRLASTLFITEGDSASGSITKARDVNTQAVFSLKGKPLNAYGLTKKVV
                     YENEEFNLIQAALDIEDGLENLRYNNIVISTDADVDGMHIRLLLMTFFLQFFPDIVKN
                     GHVYILQTPLFRVRNKKETIYCYSEDERKKAVAKLGPKPEITRFKGLGEISPDEFKHF
                     IGKDIRLEPVIIGGGAKIPDLLSYYMGKNTQERQDFIIDHLRVEKDVVETV"
     gene            complement(1936..2157)
                     /locus_tag="VFU15_00115"
     CDS             complement(1936..2157)
                     /locus_tag="VFU15_00115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716200.1"
                     /translation="MQEIFYVLFFLTVWFIVPFFAGRWLFGKLKKKFCGLGNEKGAGR
                     IFGYVAFLLCALLSWFVEVLVLTGIKMIL"
     gene            complement(2160..>2643)
                     /locus_tag="VFU15_00120"
     CDS             complement(2160..>2643)
                     /locus_tag="VFU15_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002672862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF933 domain-containing protein"
                     /protein_id="HEU4716201.1"
                     /translation="CNVDEASVKTGNAHTKAVLEAVKDEHAEVLLITAAMEAEIAALE
                     SYEDRKAFLAEIGLDEPGVNKLIHAAYRLLDLQTYFTAGVKEVRAWTITRGMTAPEAA
                     GVIHTDFEKGFIKAEVISYADFISLGSEAACRDNGKIRIEGKEYIVQDGDVMHFRFNV
                     "
BASE COUNT          548 a          744 c          655 g          696 t
ORIGIN      
        1 ttgaattagt ccgtgatcgt gcaaaccgac cgcgggaatt aaactgtttc taccacgtcc
       61 ttttccacgc ggaggtgatc gatgatgaaa tcctgccgct cctgcgtgtt ctttcccatg
      121 taataagaaa gcaggtcggg aatttttgca ccgccgccta tgatcaccgg ttcgagacgg
      181 atgtctttcc cgataaaatg cttgaactca tcgggagaaa tctctcccag ccctttgaag
      241 cgcgtaatct ccggtttcgg accgagcttc gccacggctt tttttcgttc atcttccgaa
      301 tagcagtaga tcgtttcttt tttattgcgc acacggaaca gcggcgtctg gagaatatac
      361 acgtgaccat tcttcacgat gtccgggaaa aactgcagga agaacgtcat cagcagcagg
      421 cggatatgca tgccgtcgac gtcagcgtcg gtggaaatga cgatgttgtt gtagcgaagg
      481 ttctcgaggc cgtcctcgat gtcgagcgcc gcctggataa ggttgaactc ttcgttctcg
      541 tacacgactt ttttcgtcag gccgtaagcg ttcagcggtt ttcccttgag actgaagaca
      601 gcctgcgtgt tcacatcacg cgctttggtg atcgacccgc tggcggaatc gccttccgtg
      661 atgaacaatg tcgaggcgag acggcgttcg tctttcaggt ccgtgagatg aacgcgacag
      721 tcgcgcagct ttttattatg caggttggct tttttcgcac gctcgcgcgc gagcttctga
      781 atgccggaca gctccttgcg ttcgcgttcc gactggagga tcttccggtg aagcgcatcg
      841 gcggtctcgg gaaatttgtg caggtaatta tccagctcga ccttcaggaa atcgtaaacg
      901 aacgagcgca tcgaagggcc tttcggtcct acatcctgcg agcccagctt cgttttcgtc
      961 tgtgattcaa aaacaggctc ctgcactttc acgctgatcg ccgcaacgat cgacgtgcgg
     1021 atatcgttcg cctcgtattc ttttttcagg aagtcgcgga tcaccctcac caccgcttcg
     1081 cggaaagcgc cctggtgcgt tccgccctgc gtcgtgtgct gcccgttgac gaacgaaaaa
     1141 tattcttcaa cgtattgctg atcgctgtgc gtcatcgcca cttcgatgtc gttcccgcgc
     1201 aggtggatga tcgggtaaat gatcgggccg ctgaggtttt tctggagcag atcgagcagg
     1261 ccgttgtccg acttgatcat gttcccgttg taccagagcg tgaggccggt gttcaggtag
     1321 gcgtaattcc acagcatctt ctcgacatat tcgttgatga agtggtagtt gaggaaaact
     1381 ttgtcgtccg gcttgaacga gatgatggtt ccttccgcct cgtcgcaggc ttttacgcgt
     1441 tcgtcctttt caagatttcc gcgtttgaat tcggcgacct tcgtttttcc ttcgcggatg
     1501 gactgcacgc ggaaccagga tgaaagcgca ttcaccgctt tcgttcccac gccattgagc
     1561 ccgacggatt tcttgaacgc ttccgaatca tattttgcgc cggtattgat cttcgacacg
     1621 cagtcgatca cttttcccag cgggatgccg cgcccgtaat cgcgcacggt tacgatcccg
     1681 tccttcaccg aaacctcgat ccgttttcca tgtcccatca cgaactcatc gacggaatta
     1741 tcgagcactt ctttcacgag aatataaatt ccatcgtcgg tggaagagcc gtcgcccagc
     1801 ttaccgatgt acatcccggg acgcatgcgg atgtgatctt tccagtcgag cgaccggatg
     1861 ctgtcttccg tgtaattatg tttttctgcc atcttctctc agtttaaatg gtgacctcat
     1921 tcgcttcacc aacagttata aaatcatttt tatcccggtg aggacaagca cttccacgaa
     1981 ccacgagagc agggcgcaca aaagaaaagc aacgtagccg aatattcttc ctgcgccttt
     2041 ttcatttccg agcccgcaga atttcttttt cagctttccg aacagccacc ttccggcaaa
     2101 gaacggaacg atgaaccaca ccgtgagaaa aaaaagaacg tagaatattt cctgcatgct
     2161 cagacattga acctgaaatg cattacgtct ccgtcctgga cgatatattc cttcccctct
     2221 atcctgatct tcccgttatc gcggcaggcc gcttccgaac cgagcgaaat aaaatcagca
     2281 tagctgatca cttccgcttt gataaatcct ttttcaaaat ccgtgtggat gacgcccgcg
     2341 gcttccggcg ctgtcatgcc gcgtgtgatg gtccaggcgc ggacttcctt cacacctgcc
     2401 gtaaaatacg tttgcaggtc gagcagccgg tacgccgcat gaatgagctt gttcactccc
     2461 ggctcatcga gcccgatctc ggcaaggaac gccttgcggt cttcatagct ctccagcgcg
     2521 gcgatctccg cttccatagc cgcagtgatc agcaggactt ccgcatgctc gtccttcacg
     2581 gcttcgagca ccgccttggt atgcgcgttc ccggttttca cggaagcttc atccacgttg
     2641 cac
//
LOCUS       DASTSF010000006         2396 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_2004322,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000006 DASTSF010000000
VERSION     DASTSF010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2396
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2004322"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..117
                     /locus_tag="VFU15_00125"
     CDS             <1..117
                     /locus_tag="VFU15_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002678673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716202.1"
                     /translation="GVQFHPEYKSTVDNPHPLFVSFVRAAVGVKNAVKESVK"
     gene            complement(295..552)
                     /locus_tag="VFU15_00130"
     CDS             complement(295..552)
                     /locus_tag="VFU15_00130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716203.1"
                     /translation="MSEPAAFPAYRKYKNGLSFFRINSVSSFDEIRKVGSRFLFSHHE
                     VVIFPDRNLLNDLLSAYHDFALEITREEWEDALERVEIKPE"
     gene            651..>2396
                     /locus_tag="VFU15_00135"
     CDS             651..>2396
                     /locus_tag="VFU15_00135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716204.1"
                     /translation="MENSFLENLGRYITEFYGDDTGRLCVVLPNKRAGLFLERALSSL
                     LESPSWSPEVFSVEELINDISGLTPADQAEQLVLFYSVYKKAEKEKAEPFELFSKWAP
                     TLLNDFNEADMYLADTEKLFGNLAEIKSIENWSLGAEELTEFQKEYVRFWSNIGRWHR
                     VYRDLLLREKKAYPGMMYRHVADNIAQLFPQGKWKKIIFAGFNALSAAEEKIISVLRQ
                     NRQADVLWDADRYYLEDPMQEAGKFLRAHREHLFRPLPGETASFTHVSDHFLSDEKKI
                     TVIGAAKNVSQARAAGWFLQTIPEGERFTPSTAIVLADEQLLFPVLSALPAGDDPVNI
                     TMGFPMKNSGAALFFSALFSLHETAQRFGITSRKGNRKYYHEDVIRLLKHPYTKELFP
                     GSRFTAELENMIRRMNLIFLSVEQLDLLNGFEDEKQIFSVFFEPWENGHDALSRTEAI
                     IFLLQNAFSGEQEEKMNTEREYLFRVHVMLNRVRSLADRLGDENSIHTTRAFFEQQMN
                     SATLPFSGEPLGGLQIMGLLETRTLDFRNVILLSANENILPAGKSQQSFIVHDLRRAF
                     GLPLWNEKDAVTAYHF"
BASE COUNT          578 a          648 c          625 g          545 t
ORIGIN      
        1 ggcgtgcagt tccaccccga atacaaaagc acggtggaca acccgcaccc gcttttcgtc
       61 agcttcgtgc gtgccgccgt gggtgtcaaa aatgccgtta aggaaagcgt aaaatgaaac
      121 ggcgcccgtg gggtttaacg gacgccgttc attggattgg ggttgatttg ttcaggttat
      181 agggatgtgt tcgccgcccg aataattaca ttttcatcac gcatcaaata taccagcctg
      241 cgcaatgggg cacatctacc gtttagggaa cggtcgattt gaagaaggaa gcggctattc
      301 cggctttatt tccacccgtt ccagggcgtc ttcccactct tcccgcgtta tttcaagagc
      361 aaaatcatgg tatgcgctta acaggtcgtt cagcaggttc cggtccggga aaatgacaac
      421 ctcgtgatgc gaaaacagga agcgggaacc tactttcctg atctcatcga atgacgaaac
      481 cgaattgatc ctgaaaaacg aaagaccgtt tttgtattta cggtaagcgg gaaaagctgc
      541 gggttcgctc ataattttcc ggttcaggtt gcaaaggtga atttcagcag gcagaaatta
      601 tgaccgatat tcccttgttt ttcagatatt gattcaaaat tacgcgttcg gtggaaaatt
      661 ctttcctgga aaatctcggc aggtatatta cggagtttta cggcgacgat accggccgcc
      721 tatgcgtagt gcttcccaac aagcgggcag gactttttct cgagcgcgcc ctgtcttccc
      781 tgcttgaatc gccttcctgg tcgccagaag tgttctcggt cgaagagctg atcaacgaca
      841 tttcgggact tacacccgcc gaccaggccg agcagctggt tcttttttat tcggtttaca
      901 aaaaagccga aaaggaaaaa gcggaaccgt tcgagctgtt ctccaaatgg gcgcccaccc
      961 tgctgaacga tttcaatgaa gcggacatgt atctcgccga cacggaaaaa ctgttcggca
     1021 acctcgcgga aataaaaagc atcgagaact ggagcctcgg cgcagaagag ctgacggaat
     1081 tccagaaaga atacgtccgc ttctggtcga acatcggccg gtggcaccgc gtttaccgcg
     1141 acctgctgct tcgcgaaaaa aaagcgtacc cgggaatgat gtacaggcat gtcgccgaca
     1201 acatcgcgca gcttttcccg cagggaaaat ggaagaagat tattttcgcc ggcttcaacg
     1261 ccctcagcgc cgcggaggaa aaaataattt ccgtgctgcg ccagaaccgc caggccgacg
     1321 tgctgtggga cgccgaccgt tattacctcg aagacccgat gcaggaagcg ggaaaatttc
     1381 tccgcgccca ccgcgaacat cttttccgtc cgcttcccgg cgaaaccgct tcgttcacgc
     1441 acgtgtccga tcactttctc agcgacgaaa aaaagatcac cgtcatcggc gcggcgaaaa
     1501 atgtttcgca ggcccgcgcg gccggctggt tcctgcagac aattcccgaa ggagaacgct
     1561 tcactccttc caccgcgatc gtgcttgccg acgagcagct tctttttccc gtgctcagcg
     1621 cattgcctgc cggcgatgat cctgtgaaca tcaccatggg attcccgatg aaaaattcgg
     1681 gtgcggcact tttcttttcc gcgcttttca gcctgcatga aaccgcgcag cgcttcggga
     1741 tcacgagccg gaaaggaaac cggaaatatt accacgaaga cgttatccgt ctcctcaagc
     1801 atccgtatac caaagagctt ttcccgggca gccgcttcac agcggagctg gaaaacatga
     1861 taaggcggat gaatctcatt tttctttccg tcgaacagct tgacctgctc aacggtttcg
     1921 aggacgagaa acagatcttc tcggtgttct tcgaaccgtg ggagaacggg catgacgcgc
     1981 tgtcgcggac ggaagcgatc attttcctgc tgcagaatgc attttcaggt gagcaggaag
     2041 agaagatgaa caccgagcgg gaatacctct tccgcgtgca cgtcatgctc aaccgtgtcc
     2101 gctcgctggc agacaggctt ggcgacgaga acagcatcca tacgacgcgt gcctttttcg
     2161 agcagcagat gaattcggca acgttgccgt tctccggaga accgctcggc gggctgcaga
     2221 tcatgggatt gctggaaacg cgcacgctcg atttcaggaa cgtgatcctt ctttccgcga
     2281 atgaaaatat tcttccggcc ggaaaatcac agcagagctt tatcgtgcat gatctccggc
     2341 gggcgttcgg acttccgctc tggaacgaaa aagatgccgt taccgcgtac catttt
//
LOCUS       DASTSF010000007         2088 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_883349,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000007 DASTSF010000000
VERSION     DASTSF010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_883349"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..977
                     /locus_tag="VFU15_00140"
     CDS             <1..977
                     /locus_tag="VFU15_00140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716205.1"
                     /translation="TLTDKFVKNSWFYCNARHVDGLDGAEAGFEFTDRKQKNKFTVSY
                     KMMYRADSSDLNYLVFGNEWTPGKFNNTVNFGYDHPYSYHKGKGDIRMNLRSSSIGSD
                     YNYQYMNLCVVNKNDLGKININTRFFAQIGTGTNWAKESMLFAAGENPEEMMDSKYTR
                     SMGLFPTEWADFGTDVNHFQEGGGVGLRGYAGYYMPQEDWYGDVRKTYKGTTGYGFST
                     EIEFQELFGFIGKAMPRVNSIFHLDTYLFGDIGSINFNDPGEKLAMSDFRADAGVGTA
                     LTIKRFPPLQTVKPLTIRFDMPLFLNSVPYVSQNYVQFRWVVGISRAF"
     gene            complement(1045..>2088)
                     /locus_tag="VFU15_00145"
     CDS             complement(1045..>2088)
                     /locus_tag="VFU15_00145"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEU4716206.1"
                     /translation="DVDGQGNTARFFVPTGVCFANGYVYVADAGNNKIKKIDGQGVVT
                     TIAGSGTAGFADSSALLCKMNNPTDVVVDNNGVVYFADYGNHCIRKIQGGAVTTIAGL
                     GGNSGDILGAAPAARFNRPRSLCMDGAGNIYIADLMNNKIKVLSATSNIVSLVAGSIQ
                     GYVDGAGASAEFYHPTGIDIDMWGNLVIADAGNNCVRRCTLAGVVSTLAGTGASGYAD
                     GPVLSSTFNDVEGISADAQGNVYVGDKANNVIRKLTTVDVGVFAPVNSMDLLLFPNPV
                     TDKLIIRDNAGDDALSLVQIFSVEGRLVKQISVGEYKGDFTIDISDLAAGLYDVVATT
                     QNDNVLSSRITKE"
BASE COUNT          528 a          566 c          509 g          485 t
ORIGIN      
        1 gcacgctcac cgataagttc gtgaagaact cgtggttcta ctgcaacgcg cgccatgtgg
       61 acgggctcga cggtgcagaa gccggcttcg agttcaccga ccggaagcag aagaacaaat
      121 tcacggtcag ctataaaatg atgtaccgcg ccgattcttc cgacctcaac tatcttgttt
      181 tcgggaacga atggacgccg ggcaagttca acaacacggt caatttcggt tacgaccatc
      241 cctattccta tcacaaagga aagggcgaca ttcgcatgaa cctgcgttct tcttccatcg
      301 gcagcgatta caactaccag tacatgaacc tgtgcgtggt gaacaagaac gatctcggga
      361 agatcaatat caatacgcgg tttttcgcgc agatcggaac aggaacgaac tgggcgaaag
      421 aatcgatgct ttttgcggcg ggagaaaacc cggaagagat gatggacagc aaatacacgc
      481 gatcgatggg actgttcccg acggaatggg ccgatttcgg aactgacgtg aaccatttcc
      541 aggaaggcgg cggtgtcggc ctgcgcggct atgcaggtta ttacatgccg caggaagact
      601 ggtacggcga cgtccgcaaa acatacaaag gcacgacggg ttatggcttc agcacggaga
      661 tcgagttcca ggaactgttc ggatttatcg gcaaagcgat gccgcgcgtg aacagcatat
      721 tccatctcga cacctatctt ttcggcgaca tcggctcgat caatttcaac gatcccggcg
      781 agaagctcgc gatgtccgat ttccgcgcag atgcgggcgt gggcaccgcg ctaacgatca
      841 aacgtttccc gcccctgcag accgtgaagc cgctgacgat ccgtttcgac atgccgctgt
      901 tcctgaacag cgttccgtac gtgtcgcaga attacgtgca gttccgctgg gtggtgggca
      961 tcagcagggc gttttgagga tttaaatttt ttctttcata aaaaaggccg gtattcccgg
     1021 ccttttttaa ctgtctgaat cactttattc tttcgtgatc ctggaagaaa gcacgttatc
     1081 attttgagtt gtcgccacaa catcataaag cccggcggca agatcagaaa tatcgattgt
     1141 aaaatctcct ttgtattcac caacggatat ttgttttacc aggcggcctt cgaccgaaaa
     1201 aatctgcaca agtgataagg catcatcacc ggcattgtcg cggatgatca gtttgtcagt
     1261 aacaggattg ggaaacagta acaggtccat gctgttcaca ggcgcaaaaa cacccacatc
     1321 taccgtggta agttttctga tcacattgtt cgctttgtcg ccaacataaa cattaccctg
     1381 tgcatctgcg ctgatccctt caacatcatt gaacgtagag ctcaatacgg gaccatccgc
     1441 gtaccccgat gcccctgttc ctgccagcgt agacactacg cctgcaagtg tgcacctgcg
     1501 aacacaatta ttgcctgcat cagcgataac aaggtttccc cacatgtcga tgtctatccc
     1561 ggtagggtgg taaaattccg cagaggctcc ggctccgtcc acatagccct ggatggatcc
     1621 ggccacaaga gaaacaatat tggaagtggc ggaaagcact ttgattttat tattcatcag
     1681 gtccgcgata taaatattcc cggccccgtc catgcataaa gaccgcggac ggttaaaacg
     1741 ggccgcaggc gcagcgccaa ggatatcccc ggaatttcct cccaagccgg ctatcgtggt
     1801 aacagcccca ccttggattt tgcgaatgca gtgattcccg taatccgcga aatacaccac
     1861 cccgttatta tccacgacga catccgtcgg gttgttcatc ttgcagagca gggcgctgct
     1921 atctgcaaaa cccgccgtac ccgatccggc aatggttgtc acaactccct ggccgtctat
     1981 cttttttatt ttattgttac cggcatcagc cacgtaaaca taaccattag cgaaacagac
     2041 acctgtcggt acaaagaacc gtgccgtatt gccctgcccg tcaacatc
//
LOCUS       DASTSF010000008         5994 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1777018,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000008 DASTSF010000000
VERSION     DASTSF010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5994)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5994
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1777018"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..368)
                     /locus_tag="VFU15_00150"
     CDS             complement(<1..368)
                     /locus_tag="VFU15_00150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716207.1"
                     /translation="MKKILLLFFFALAAHLLHAQRFHRRAVYVNKWYSREIVKTGAKA
                     YCLYLNGMNEAGGGEAIEFGTDVPLFVWEKQYYFLVDADWRLAAAKHSSAGVGGSVAG
                     EFRFYNDARRQASWGMEFSWG"
     gene            complement(445..2262)
                     /locus_tag="VFU15_00155"
     CDS             complement(445..2262)
                     /locus_tag="VFU15_00155"
                     /inference="COORDINATES: protein motif:HMM:NF024974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HEU4716208.1"
                     /translation="MKQRYPLLLALAFSASAGLHAQIFYSNGATVAVTTGAVVWCNGG
                     ATLDNTTNFTNNGSFTTANSTTPAVMPGTFTIDNSSAAQGNGTYQVEQDWVNNATFVD
                     GTSTVRLFGNTQQFITSTNNTVTTFNNLTLLGNGTGNNRKKTLQGVNAISDVGGVDSI
                     NNRELETLTQTFFVTNPATTAVYNDQTANAEGFVSSQAPGTFSRVTSGSGAYLFPTGS
                     SVVLTRYRPVMLTPVAGSTNDIYTCRFINHDPDNDGFLRSTNDGLICNANDTFYHAIN
                     RPGGTDAADVTIYYIPANDGAWSGISQWRLSNVMWNDMAATSQATTGIFTTRTRSAWG
                     FVNPGDPYVLNNQRPAAPNIVCPSICQNSSANLFVATGGSGYQWTVPGNGTIVSGQGT
                     DSLYVDWTTGSGPVTVVATGANCNSLPSSCTPTILPSPFANFLDTSSGPWAGTWVFSD
                     QSTVPAPDNIVSWTWDFGDGQFSTQQNPSHDFNGSGTYTVTLTATSGAGCTDTVQSVV
                     TVLEGILIPNVFSPNGDGVNDEFYIPNSGLKEYKIEIYDRWGVKVFESDAPDIHWDGR
                     STSGQPCTDGTYYYILHAVSNVADYSTTGFVTLIGSKKQ"
     gene            complement(2348..2677)
                     /gene="gldC"
                     /locus_tag="VFU15_00160"
     CDS             complement(2348..2677)
                     /gene="gldC"
                     /locus_tag="VFU15_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014679054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldC"
                     /protein_id="HEU4716209.1"
                     /translation="MKKAQITFTVTLDENSLPESIEWKASDSGEGGNCKAIMLAMWDE
                     KEQSTMRIDLWTKQMLVDEMKKFFHQNLVTMSDTFLRATGEEKIVGDMRDFCAYFAEK
                     MEIFSQS"
     gene            complement(2723..3667)
                     /locus_tag="VFU15_00165"
     CDS             complement(2723..3667)
                     /locus_tag="VFU15_00165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716210.1"
                     /translation="MSSISVPPVKIMRYDEAFFSIDTNNIPASLDKLRKQFGPFSDGF
                     VNNVVCSDAHDSISCDMAIRDFILSYDMRSAWKDSKNILGDQSALEAQITDAYRHFRY
                     HFPKRDLPKAVYIDFTGFNYNILNIEGVYGIGLEFYLGKDNVIYDGLSDHFPGYIRRN
                     CTKEYAVANFVRGWMMNEFPYDPPKNDLINKMVWEGKMMYLMKALLRNTPDSIITGYT
                     AKQLSWCADNEAKMWATLIENKKVYSDNDEDLQHFTENGPFTPDFPRDSPGEAGNWLG
                     LRIVEAYMARNPNVSLQQLMDQKDGSFILTRAKYKPKF"
     gene            3821..4603
                     /locus_tag="VFU15_00170"
     CDS             3821..4603
                     /locus_tag="VFU15_00170"
                     /inference="COORDINATES: protein motif:HMM:NF024957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEU4716211.1"
                     /translation="MKKTFLLLLVSAFAGKVLFAQDGENDLKNFRFGLKATGMLTWYK
                     PDDKKKYESNGVSTKGSYGLSTEFRLNKVAVIATGLQVDYDGGKLNMLDTNYYFLSKD
                     SQFMTKSDTAGQHFDTYWLRSRTYNTTYLTIPLTLKLKTNEIGFLTYYGQFGVNASFK
                     LKSRVNDKVTTTSLGSADQKDLDNTSDMSLFRFALNIGGGAEYNLSGSTSMVFGINWY
                     NGFSNVIKSDSKYLFRTASNSYAATPQKATSNAIALTVGVLF"
     gene            4661..5416
                     /locus_tag="VFU15_00175"
     CDS             4661..5416
                     /locus_tag="VFU15_00175"
                     /inference="COORDINATES: protein motif:HMM:NF016241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HEU4716212.1"
                     /translation="MKRFLFAAFLLLTAAVVHSQVLNIESMRFYKDSDGWIGSANLDF
                     GAQQNVNTLYDFSNTIHVQYKKKRSRIIFLNNLDFTFANKQDYENTGFQHVRYNYKLS
                     DNGRWTAEAFVQTQYNKPMKMDFRGVTGAGIRMRVFKTKIVHMYLGSLYMFEHETDDG
                     GKLVYDDHRLSSYLTFSVDFNGKAEINNTVYYQPAFVNFSDYRISESFTCLAQITRHF
                     GFSAAFDLLYDTKQPEGIPNLSWKFEQGIAYSF"
     gene            5451..>5994
                     /locus_tag="VFU15_00180"
     CDS             5451..>5994
                     /locus_tag="VFU15_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016197432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HEU4716213.1"
                     /translation="MKIALAQLNYHIGNFTFNRSQIISFIHRAKKEGADLVVFSELSV
                     CGYPPKDFLEFRDFIRRCEESVELIAKECTGIAAIVGAPAVNPVTEGKDLFNAAYVLE
                     NGKIKSVHHKGLLPNYDVFDEYRYFEPGKKFHCAEVKGKKIALTICEDLWDVFDDQMY
                     TQWPMEELKKENPELMINIAA"
BASE COUNT         1460 a         1536 c         1605 g         1393 t
ORIGIN      
        1 ccccaggaaa attccattcc ccagctggcc tgcctgcgcg catcattgta aaaacggaat
       61 tcgcccgcta ctgatccgcc caccccggcg gaactgtgct tcgctgctgc cagtctccag
      121 tccgcatcga ccaggaaata atattgcttt tcccatacaa aaagcggaac atccgtaccg
      181 aattcgatcg cctcgccacc gccggcttca ttcatgccgt tgaggtaaag acaataagcc
      241 tttgccccgg tcttcacgat ctcgcggctg taccatttgt tcacatacac ggcacgccgg
      301 tgaaaacgct gggcatgcaa caggtgagct gcgagggcga agaaaaaaag caggagtatc
      361 tttttcattt ctctcagggt acgggtcaca aaaaagccgc cacctcattg agggaggggc
      421 ggcttttcaa taaaattttt cgtgctattg cttcttcgaa ccgatcaggg tcacaaaacc
      481 tgttgtgctg taatcggcaa catttgaaac ggcatgcagg atgtagtaat aggttccgtc
      541 cgtgcagggc tggccggaag tagaacgtcc gtcccagtga atgtcgggcg catcggattc
      601 aaatactttc acaccccagc ggtcgtagat ctcgatcttg tattctttca gtccgctgtt
      661 gggaatgtaa aactcatcgt tgacgccgtc accgttcggt gagaatacgt tcgggataag
      721 aattccttcg agcaccgtca ccactgactg aaccgtatcc gtgcatcctg caccgctcgt
      781 agcggtgagc gtgacggtgt aagtgcccga tccgttgaaa tcatgcgaag gattctgctg
      841 cgttgagaat tgtccgtcgc cgaaatccca ggtccagctc acgatattat caggagcagg
      901 aaccgttgac tgatcgctga acacccatgt tcccgcccag gggccggaag aagtgtcgag
      961 gaaattcgcg aacggggaag gtaaaattgt cggtgtgcag gaagaaggaa gcgagttgca
     1021 atttgcgccg gtcgctacaa ctgtgacagg tccgcttccg gttgtccagt caacataaag
     1081 cgaatccgtt ccctggccgg aaacgatcgt gccgttgccg ggaaccgtcc actggtatcc
     1141 cgatccgccg gtggcaacga aaaggttcgc gctcgaattc tggcagatcg aaggacatac
     1201 gatgttcgga gcggccgggc gctggttgtt cagcacatag ggatcacccg ggttgacaaa
     1261 gccccacgcg gaacgcgtgc gcgttgtgaa aataccggtt gttgcctgcg acgttgccgc
     1321 catgtcgttc cacatcacat tgctgagtct ccactgggag atgccgctcc atgcgccgtc
     1381 gttggcagga atgtaataga tggtgacatc cgccgcatcc gttccgcccg ggcggttgat
     1441 cgcgtggtag aatgtatcgt ttgcgttgca gatgagaccg tcattggtgg aacgcaggaa
     1501 accgtcgttg tcaggatcgt ggttgatgaa acggcaggtg taaatatcat tcgtagaacc
     1561 ggcaacaggc gtaagcataa ccgggcggta gcgtgtaagc acaacgctgg atccggtagg
     1621 gaaaagataa gctccggatc cgctggtcac acgtgaaaaa gttcccggcg cctggctgct
     1681 gacgaatcct tccgcgttcg cagtctggtc gttgtacacc gcggtggttg cgggattggt
     1741 gacgaagaaa gtctgggtaa gcgtttcgag ctcgcggtta ttgatggaat cgacgccgcc
     1801 gacatcgctg atggcgttca cgccctgcaa tgtttttttg cggttgttgc ccgttccgtt
     1861 tccaagcagc gtgaggttgt tgaatgtcgt aacggtgttg ttggtgctgg taatgaactg
     1921 ctgcgtattc ccgaaaaggc gtaccgtgct ggttccgtca acgaacgtcg cgttgttcac
     1981 ccagtcctgc tccacctggt aggttccgtt cccctgcgca gccgaagagt tgtcgatcgt
     2041 aaacgttccg ggcattacgg ccggcgtggt ggaattcgcg gtggtgaatg aaccgttgtt
     2101 ggtgaagttc gtcgtattgt caagcgttgc gcctccgttg caccagacaa ctgcaccggt
     2161 cgtgacggca acagtcgctc cgttggagta aaagatctgg gcatggagtc ctgcagaagc
     2221 ggaaaaagcc aatgcaagca acagggggta cctttgtttc atggttaatg cggtttaaac
     2281 cgagccaaaa acaaaataaa gactttttcg cgagaaaaaa aaggccggaa tgctgaaatt
     2341 cagagggtca ggactggctg aagatctcca ttttttcggc gaaataagcg cagaaatcgc
     2401 gcatatcgcc gacgatcttt tcctcgccgg tggcgcggag gaacgtatcc gacatggtga
     2461 caaggttctg gtggaagaac ttcttcattt catcgaccag catctgcttg gtccacagat
     2521 caatacgcat cgtactctgc tccttttcat cccacatggc cagcatgatc gccttgcagt
     2581 ttccgccttc gccggaatca ctggctttcc attcgatgga ttcagggagg ctgttttcgt
     2641 cgagcgttac cgtgaacgtg atctgtgctt ttttcatgcg ccaaagataa gcggagaaca
     2701 gcgaacggcg ggaggccggt tgtcagaact tcggcttgta cttcgcgcgg gtgagaatga
     2761 aggatccgtc cttctggtcc atgagctgct gcagggaaac attcggattg cgcgccatgt
     2821 aggcttcaac gatgcgcagg ccgagccagt ttcccgcttc gccgggcgaa tcgcggggga
     2881 aatcgggggt gaacggacca ttctcggtga aatgctggag gtcttcgtca ttatccgaat
     2941 acaccttttt attttcgatc agcgtggccc acattttcgc ttcgttgtct gcgcaccagc
     3001 tgagctgctt cgcggtgtag ccggtgatga tgctgtcggg cgtattccgc agcagggctt
     3061 tcatcaggta catcattttt ccttcccaca ccattttatt aatgaggtcg tttttcggcg
     3121 gatcgtaggg aaactcattc atcatccacc cgcggacaaa attcgccacc gcatattcct
     3181 tcgtacagtt gcgcctgatg tagcccggaa aatgatcgga aagtccgtcg tagatcacat
     3241 tgtctttccc gagataaaat tcaagcccga tcccgtaaac gccttcgatg ttcagtatat
     3301 tataattgaa tcctgtaaaa tcgatgtaaa ccgctttcgg gagatcgcgt ttcggaaagt
     3361 gataacggaa atggcggtac gcatcggtga tctgcgcttc gagcgcggac tggtcgccga
     3421 gaatgttttt cgaatccttc caggcagagc gcatgtcgta gctgagaatg aaatcgcgga
     3481 tggccatgtc gcagctgatg gaatcgtgtg cgtcgctgca gacgacatta ttgacaaagc
     3541 cgtcggaaaa aggtccgaac tgtttccgca gcttatccag cgaagcggga atgttattcg
     3601 tatcgatgga aaaaaaggcc tcgtcgtaac gcatgatctt cacgggaggc acggaaattg
     3661 atgacacgtc gacgtccagc ggatcgcgat ggcacgaaat taaaaccaga ttaaaaagaa
     3721 acagaacagg ccatgaacgg cgcaggacag tgtgaaaacc tctcataatt cagtccaaaa
     3781 ttatcgtata attgcacaac gaaaacccag cactaaaagt atgaaaaaga catttttact
     3841 tctactggta tctgcatttg ccggcaaagt tctttttgcg caggatggag aaaacgacct
     3901 caagaatttc cgcttcggcc tgaaagcaac cggcatgctt acctggtaca agccggatga
     3961 caagaaaaaa tacgaaagca acggcgtttc cacgaaaggc agctatggtc tttccacgga
     4021 attccgcctg aataaagtag cggtgatcgc cacggggctg caggtggatt atgacggcgg
     4081 aaagctgaac atgctcgata caaattatta tttcctttcg aaagacagcc agttcatgac
     4141 caaatcggat acagccgggc agcatttcga tacgtactgg ctgcgcagcc gtacttataa
     4201 cacaacgtat ctcacgatcc cgctgacgct caagctcaag accaacgaga tcggtttcct
     4261 cacctattac ggccagttcg gcgtcaatgc ttcgttcaag ctgaaaagcc gcgtgaacga
     4321 caaggtcacg acaacaagcc tcggctccgc cgaccagaaa gaccttgaca atacgagcga
     4381 catgagtctt ttccgctttg cgttaaacat cggcggcggc gccgaataca acctttccgg
     4441 atcgacctcg atggttttcg gcatcaactg gtacaacggg ttctcgaacg tcatcaagtc
     4501 ggattcgaaa tatcttttcc gtacggcatc gaacagttac gcggcaacac cacagaaggc
     4561 aacttcgaat gcgatagcgc ttactgtcgg cgttcttttc taaacgaaaa atgcttcact
     4621 ctcaaatggc cggttcattc cggccttttt tatttgcacg atgaaacgtt ttctttttgc
     4681 cgcatttctc ctgctgactg ctgccgttgt tcattcgcag gtgctgaaca tcgagtcgat
     4741 gcgtttctac aaagattcgg acggttggat cggcagcgcg aacctcgact tcggcgcgca
     4801 gcagaacgtg aacacgctat acgatttctc gaacaccatc cacgtgcagt acaaaaagaa
     4861 acgcagccgc atcattttcc tgaacaacct cgatttcact tttgcgaaca aacaggatta
     4921 tgagaacacc ggcttccagc atgtccgcta taattacaag ctgagcgaca acggacgctg
     4981 gacagcggaa gcgttcgtac agacgcagta caacaaaccg atgaagatgg atttccgcgg
     5041 tgtgacggga gccgggatac ggatgcgcgt attcaaaacg aagatcgtgc acatgtacct
     5101 cggttcgctg tacatgttcg agcacgagac cgacgacggg ggaaagcttg tttacgacga
     5161 tcaccggctc agctcgtacc tgacgttctc ggtcgacttc aacggaaaag cggagatcaa
     5221 caacacggtc tattaccagc ccgcattcgt gaacttcagc gattaccgca tttcggaaag
     5281 tttcacgtgc ctggcgcaga tcaccaggca tttcggattt tccgcggcgt tcgacctgct
     5341 gtacgacacc aagcagccgg aaggcatccc gaacctgtcg tggaaattcg aacaggggat
     5401 cgcctacagc ttctgacgcc tgcggcggaa aatcgcgtta actttgcggc atgaaaatcg
     5461 cgctggcgca gctgaattac cacatcggca atttcacgtt caaccgttcg cagatcattt
     5521 cctttattca ccgcgcgaaa aaagaaggcg ccgaccttgt cgttttttcc gagctttccg
     5581 tctgcggcta cccgccgaaa gattttctcg agttccgcga tttcatccgg cgctgcgaag
     5641 aatcggtgga gctgatcgcg aaagaatgca cgggcattgc tgcgatcgtg ggtgcgcctg
     5701 cagtgaaccc ggtgacggaa ggcaaggatc ttttcaatgc ggcgtacgtt ctcgagaacg
     5761 ggaaaataaa aagcgttcat cataaaggat tgctgccgaa ctacgacgta ttcgacgaat
     5821 accggtattt cgaaccgggg aaaaaattcc attgcgcgga agtgaaaggg aaaaagatcg
     5881 cgctgaccat ctgcgaggac ctgtgggacg tgttcgatga ccagatgtac acgcagtggc
     5941 cgatggaaga gctgaagaaa gaaaatcccg aactgatgat caacatcgcg gcgt
//
LOCUS       DASTSF010000009        10656 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1554018,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000009 DASTSF010000000
VERSION     DASTSF010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 10656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10656
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1554018"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..454)
                     /locus_tag="VFU15_00185"
     CDS             complement(<1..454)
                     /locus_tag="VFU15_00185"
                     /inference="COORDINATES: protein motif:HMM:NF036423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase SAM-like domain-containing
                     protein"
                     /protein_id="HEU4716214.1"
                     /translation="MGVILREKKLKGGGVSYYLDINHEGRRWYEFLEIRVKGNRRDPE
                     NVEKKKLADKARSAREYQLTVEKSHLPNEQEKERDMVAFIKEKSAGMRSQEAPGQLCK
                     KLIAFTGWEEVPMSKIDKSFLLRFQEYLKSIGLGQGTVYTLVHRFSTYI"
     gene            767..1582
                     /locus_tag="VFU15_00190"
     CDS             767..1582
                     /locus_tag="VFU15_00190"
                     /inference="COORDINATES: protein motif:HMM:NF019284.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HEU4716215.1"
                     /translation="MKRLKINQLKLSALVLATVLSGFAAAQVPTGLYPNKTDSLGKQG
                     AWKKTDDQGTCVYVGQFKNDKPYGIFQYFDTQGYLMTEMNFFSGGNVQYAKMYYQNGK
                     MQAQGKYLNQKKDSLWSFYDDLGELLSEEWYKDGLKNGKSITYHPGTKQPAEVKTFKN
                     GLEEGAWIQYYADGKIKGQGTYVAGNYEGKATWYYPDGSTNITGFYQHAVKNGTWLYY
                     DETGKLKGKETWVNGKLTSEETVIKKADFDKQVQDDDDPATQGQDPNDGTGGN"
     gene            1586..2695
                     /gene="mnmA"
                     /locus_tag="VFU15_00195"
     CDS             1586..2695
                     /gene="mnmA"
                     /locus_tag="VFU15_00195"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEU4716216.1"
                     /translation="MSKKGKVLVAMSGGIDSSVTALMLHNEGYEVIGITMKTWDYASS
                     GGSKKETGCCSLDSINDARMLAVNLGFPHYILDIRGEFGDHIINNFVDEYLAGRTPNP
                     CVLCNTHIKWDALLKRADQLGCEFIATGHYAQVREENGRYVVSKGIDEAKDQSYVLWG
                     LSQESLKRTIFPLGKFRKSEIRDMAANAGYEELSKKSESYEICFIPENDYRAFLKHKV
                     PGLEQKVDGGDFVLSDGKVVGKHRGYPFYTIGQRKGLEIALGEPMFVTQIIPESNTVV
                     LGTKTELEKQEMLVRDYNLVKYASLPENFASTTKIRYHDPGTPASLTEAGGKIKVLFH
                     QSVSGVAPGQSAVFYEGNDVVGGGFIDRPKPVSLQ"
     gene            2692..3420
                     /locus_tag="VFU15_00200"
     CDS             2692..3420
                     /locus_tag="VFU15_00200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716217.1"
                     /translation="MKKKLRAFYVVVLITGMASAVFSSCKKDGTVDTGYSYFPDAIGH
                     YCIYDVDSTVYDDFNNDTVVYRYEVKEIIESYFTDNQGRQAMRVERYRKNYSDTVPYA
                     NQPWTISRVWSFVRTSSEAEKQEENQRYIRLTFVPREGKTWNGNAYNTLGEMDYKYAS
                     VDQPYSLNAMNFDSVATVLQEDETNLITRKFYQERYARNVGMIEKSIIDVHGRTDTLI
                     LSPVMGRITGGVVCNFRLVDWGPR"
     gene            3526..5199
                     /locus_tag="VFU15_00205"
     CDS             3526..5199
                     /locus_tag="VFU15_00205"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEU4716218.1"
                     /translation="MFRRFTFIFLCLFFASALFSQDNKHWVQFRDKGNNPYSLSNPSA
                     YLSPRALARRTTQGIAIDSLDLPVTPAYIAGVAATGATVHSVSKWLNGVIVFTNDTNV
                     LNAIAALPYVVSVNNAGMRVANPDTVNKFSEETYSPLQQQTGRSGYVTSSGLNYGPSY
                     NQISMLGGVCLHNAGYRGQGMQIAIIDAGFYHANVLPVFDTLFMNNRILGTWDFVAGD
                     SSVYEDNQHGMMVLSTMGGNDPGNLVGTAPDASYWLLRSEEAATEYIVEEYYWASAAE
                     YADSVGADVINSSLGYTEFDDPSENHTYADMNGHTTPCARAANIAARKGMALVIAAGN
                     SGQSPWFYISTPGDADSVLTVAALDASGNVAGFSSRGPASDGDVKPCTGAQGVNAVIA
                     SPTGGTQTGNGTSFATPITCGLVACLWQAHPSMTNMQLIQYIEMSASQYSNPDSLMGY
                     GIPDFCAANLALSPNPVYDQANDYLYNVVPNPFVNQLDFSFYSMTDQDIQVNLYDVQG
                     RQMIDQLVFAAKKAENHYTLTELEAAGPGVYFLVVKGNEGTYYSRIVKM"
     gene            complement(5245..6297)
                     /locus_tag="VFU15_00210"
     CDS             complement(5245..6297)
                     /locus_tag="VFU15_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010611852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HEU4716219.1"
                     /translation="MTYVRPKFRHPELPKNKLGYTQDYYEGALSTLCAGCGHDSISAA
                     IVQACFENNIEPHKVAKMSGIGCSSKTPAYFLSNSHGFNSVHGRMPSVATGANLGNRD
                     LIYFGVSGDGDTASIGMGQFVHVIRRNLNMTYIVMNNGCYGLTKGQDSATADEGSKSK
                     AGSVNPFHAIDLASLALELGATFVAQSFSGDKRQLVPLIKAAMKHKGFAFINVISPCV
                     TFNNNPGSTKSYDFVREHMEATATMDFVPEMKEITTSYEEGTVKKVEMHDGTAIQLHK
                     LEQEWDPQDRFSAIHAIKKAQDKGEILTGLLYIDENSKDLHDILNTTQKPLNTLTEKE
                     LCPGSSALADINSGLR"
     gene            complement(6302..8125)
                     /locus_tag="VFU15_00215"
     CDS             complement(6302..8125)
                     /locus_tag="VFU15_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010611853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HEU4716220.1"
                     /translation="MNEKVNDFVIRFANVNGTGSASANFLFTKAIFRMGIFVTPKNIF
                     PSNIQGLPTWYEVRVNEKGYLGRRDGVDIMVCVNPQSMKQDVASVRPGGYFLYDCTKA
                     LHEEFIRRDINYIGIPLMAMCNREFTDARQRQLFKNIVYVGALSALLNIEFKVLEDLL
                     ADQFAGKEKLIAPNVKALKMGVDYVKENFNYPLDIRVERRNLLGDSIMVDGNSACALG
                     AVYAGATVVAWYPITPSTSVAEAFSGYAEQLRVDKETGTHNYAVVQAEDELAAIGMVI
                     GANWNGARSFTATSGPGVSLMNEFLGLAYFAEVPAVLIDVQRTGPSTGMPTRTQQSDI
                     LEAAYASHGDTKHVLLFPNTPKECFEMTAEAFDLAEELQTPVMVMTDLDLGMNDHISE
                     PFKWDDKRKLRRGKVLTAEQLDKLERYGRYLDVDNDGITYRTIPGVHPTKGAFVTRGT
                     SRDEYATYTEDGAAYVRNMDRLTKKFETAKKMVPAPHLYQDKNSFENAIVFFGTSTYS
                     AEEAMDLLKEQGVYVDAMRLLAFPFSKTVEDFIHAHKRVFVIEQNRDGQMRSMLQMEL
                     GINPEKLVAVLNYDGMPITADNILKQITKHLSTTSKQKEVR"
     gene            complement(8122..9900)
                     /locus_tag="VFU15_00220"
     CDS             complement(8122..9900)
                     /locus_tag="VFU15_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010854759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU4716221.1"
                     /translation="MKPTNVKDPEYFHKVVDCQYACPAHTPVPEYIRLIAEQKYTEAY
                     MINWESNVFPGILGRTCDRPCEPACRRGRLEPNEEPVAICRLKRVAADNKGDVKQHMP
                     AGPFKPNGKKIALIGAGPASLTVARDLAPLGYEIHLYDEQKAGGGFMRSQIPSFRLPE
                     TVLEEEVNYILDLGIHTHFNTYVKSLKEVLAKDYDAVFVGSGAPKGRDLDIPGRKEGA
                     ANIHIGINWLANVAFEHTNKIGKKVIVLGGGNTAMDCCRTSRRLGGEQVKVIVRSPFA
                     EMKASPWEKEDAMHEDIPIIDNHVPKSFVIENGKLKGMMFEKVKAVYENGKRKLVPTG
                     EPEVFHEADDVLVAIGQENSFPWIERDLGIEFDKWDMPVVNETTFASTNKKVFFGGDA
                     AFGPKNVITAVAHGHQAAISIDLACRNEDLMKRPSPYVNLVSQKMGIHEWSYDNMVVV
                     DKRYMVPQADKKVTLSNRKKEVELGFDLPTGFKEAERCLNCDVETVFTENKCIECDAC
                     VDICPTSCITFTENGDESDLRTRLKAPANVESQALYVSDVLPTKRVMVKDENVCLHCG
                     LCAERCPTSAWDMQKFFYSVTKAYQV"
     gene            complement(10185..>10656)
                     /locus_tag="VFU15_00225"
     CDS             complement(10185..>10656)
                     /locus_tag="VFU15_00225"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU4716222.1"
                     /translation="MLQLVDDVFSTRSDPSQLQVDEDVLRKLGEIHPATLSDYDDGEG
                     PAVWILVIPTTEKTMRLFLDGAIPENELLARTSPGEKFEAIYLCSATVLPEFRKKGLA
                     RKLTVEAIENIRKDHGIRFLYVWPFTPEGDRLAEKIAAITGLPLLKKISGHARH"
BASE COUNT         2430 a         2736 c         2941 g         2549 t
ORIGIN      
        1 agatgtacgt gctgaaccga tgaacaagcg tataaacggt tccttgtccc aagccaattg
       61 atttcaggta ttcctgaaac cgaagcagga acgacttgtc gatcttgctc atcggcactt
      121 cttcccaacc ggtgaatgct attagctttt tgcataattg tcccggtgct tcctgcgaac
      181 gcattccagc agacttttcc ttgatgaacg ccaccatgtc gcgttccttt tcctgctcat
      241 ttggcaggtg gcttttctcg acggttagtt ggtattcgcg ggccgaacgt gctttatcgg
      301 ccagcttctt cttttccacg ttctccgggt cacggcgatt gcctttcacc cgaatttcga
      361 ggaactcata ccagcggcgg ccttcgtggt tgatgtcgag gtaataggat accccgccgc
      421 ccttcaactt cttctcacgc aaaataactc ccatgttagt ggtttttacg agttaaacat
      481 ggtaacggtt gcgggtaacg gttttagcat aaaccaaagg aaaacgctta cctaccgttt
      541 ggaggaaaac gccctgaaag ccttgtcaat attggctttt ttcgatattc tatttccttt
      601 gctttcgggg tgtactatta aaaataggaa atccgtaaaa agcaggcggt aataaatcct
      661 catcatctgc gttacttttt aaccaccaat gctttatatc ttcagatggc gcgttttttg
      721 ccattcagtc cctgaaagcc ttaactttat gtaactttgc atcctgatga aacgcctgaa
      781 aatcaaccaa cttaaactgt ctgcgctcgt gcttgcgaca gttctttccg ggttcgccgc
      841 agcccaggtt cctacgggtc tttatccgaa taaaacggat tcgctcggaa aacagggcgc
      901 atggaaaaaa acggacgacc agggaacgtg tgtttatgtc gggcaattca aaaacgacaa
      961 accttacggt attttccagt atttcgatac gcagggttac ctgatgaccg aaatgaactt
     1021 cttcagcggc ggcaacgtcc agtatgcgaa gatgtattac cagaacggga aaatgcaggc
     1081 gcaggggaaa tacctcaacc agaagaaaga cagcctctgg tcattttatg atgatctcgg
     1141 cgagctcctt tcggaagaat ggtacaagga cggactgaaa aacggaaaat cgatcaccta
     1201 tcaccccgga accaaacagc cggcagaagt gaagacattc aagaacgggc tggaagaagg
     1261 agcgtggata cagtattacg ccgacgggaa aattaaagga cagggaacgt atgtcgccgg
     1321 aaactatgaa gggaaagcca cctggtatta cccggacgga tctacgaaca taaccggctt
     1381 ttaccagcat gccgtgaaga acgggacgtg gctgtattat gatgaaaccg gaaaactgaa
     1441 aggaaaagaa acgtgggtga acggcaagct caccagcgaa gaaacggtga tcaagaaagc
     1501 cgatttcgat aagcaggtgc aggatgacga tgatcccgcc acacagggac aagaccccaa
     1561 tgacggaaca ggaggtaatt aagccgtgag caaaaaagga aaagtgctgg ttgcgatgag
     1621 cggcggtatc gacagttcgg taacggcgct catgttgcat aatgaaggtt acgaagtcat
     1681 cggcatcacg atgaagacgt gggactatgc gtcttccggc ggatcgaaaa aagaaaccgg
     1741 ctgctgcagc ctcgattcca tcaacgatgc gcgcatgctg gcggtgaacc tcggtttccc
     1801 gcattatatt ctcgacatcc gcggcgagtt cggcgaccac atcatcaaca atttcgtgga
     1861 tgaatatctc gcagggcgta cgccgaaccc gtgtgtgctg tgcaataccc acatcaagtg
     1921 ggacgcgttg ctgaagcggg ccgaccagct cggctgcgaa ttcatcgcga ccgggcatta
     1981 tgcgcaggta cgtgaggaga acgggcggta cgtcgtttcg aaagggatcg acgaagcaaa
     2041 agaccagtcg tatgtgctgt ggggactttc gcaggaaagc ctgaagcgca cgatctttcc
     2101 attgggaaaa ttcaggaaat cggagatccg tgacatggcg gcgaatgccg gttacgaaga
     2161 actttcgaaa aaaagcgaga gctacgagat ctgctttatt ccggaaaacg attaccgcgc
     2221 attcctgaag cataaagttc cgggcctcga gcagaaagta gacggcggcg attttgtgct
     2281 cagcgacggg aaagtcgttg ggaagcaccg cggctacccg ttctacacga tcgggcagcg
     2341 gaaagggctc gagatcgcgc ttggcgaacc gatgttcgta acgcagatca tccccgaatc
     2401 gaataccgta gtgctcggca cgaaaacgga actggaaaag caggagatgc tcgtgcgtga
     2461 ttacaatctc gtgaagtacg catcgctgcc ggaaaatttc gcgtccacca ccaagatccg
     2521 ttaccacgat cccggaacgc cggcttcact tacggaagca ggaggaaaga taaaagtgct
     2581 tttccaccag tcggtttcgg gtgttgcgcc cggccagtcg gctgtgtttt acgaagggaa
     2641 tgacgttgtg ggcggcgggt tcatcgatcg tcccaaaccc gtttcactgc aatgaaaaaa
     2701 aagctgcgcg cgttttatgt cgtggtgctg atcaccggca tggcgtcagc ggttttttct
     2761 tcctgtaaaa aagacgggac tgtcgataca ggctattctt atttcccgga tgctatcggg
     2821 cattactgca tttacgacgt cgattccacc gtgtatgacg atttcaataa tgacacggtc
     2881 gtttaccgct acgaggtaaa ggaaattatc gaatcgtatt tcaccgacaa ccagggacgg
     2941 caggcgatgc gcgtggagcg ttaccggaaa aattacagcg acactgttcc ttacgcgaac
     3001 cagccctgga cgatcagccg ggtgtggtcg ttcgtgcgca cgtcatcgga agcggaaaag
     3061 caggaggaga accagcgtta catccgcctg acattcgttc cgcgtgaagg aaaaacctgg
     3121 aacggaaacg cctacaatac gctcggtgaa atggattaca aatatgcatc ggtcgaccag
     3181 ccgtattcgc tgaacgcgat gaatttcgat tccgtcgcaa cggtgttgca ggaagacgag
     3241 acgaacctga tcacccggaa attctaccag gaacgttatg cgcggaatgt gggcatgata
     3301 gagaaaagca tcatcgacgt gcatggacgt acggacacgc ttattctttc cccggtgatg
     3361 ggacgcatta caggcggcgt ggtgtgcaat ttccggctgg tggattgggg accgagatga
     3421 tgaatgatta agaatcgagg gtgtaccacg aagtgaagat gtaagtggat cgcagcgcgc
     3481 ccgatcggcc cttttcggta aatttgttca accaaaaaca ggatgatgtt cagaaggttt
     3541 acgtttattt ttctctgcct atttttcgcc tccgctttat tttcccagga caacaagcac
     3601 tgggtgcagt tccgcgacaa gggaaataat ccctactcgc tgagcaatcc ttccgcgtat
     3661 ttgtcgccgc gtgcattggc gcgcaggacg acgcagggaa tcgcgatcga ctcgctggat
     3721 cttcccgtga caccggcgta catcgcgggc gtggctgcaa cgggcgcgac ggttcattcg
     3781 gtttccaaat ggctgaacgg ggttatcgtt tttaccaatg acacaaacgt gctgaatgcg
     3841 atcgccgcgc tgccgtatgt cgtgagcgta aacaatgcgg gaatgcgtgt cgccaacccg
     3901 gataccgtga ataaattttc ggaagaaacc tattctccat tgcagcagca aacgggacgt
     3961 tccgggtatg tcacttcttc cgggctgaat tacggtccgt cgtacaacca gatcagcatg
     4021 ctcggcggcg tgtgcctgca caacgcgggc taccgcgggc agggcatgca gatcgcgatc
     4081 atcgacgcgg gattttatca cgccaacgtt ctcccggtct tcgatacgct cttcatgaac
     4141 aaccgcattc tcggcacctg ggattttgtc gcgggcgatt cttccgttta cgaggacaac
     4201 cagcatggca tgatggtgct gagcacgatg ggcgggaacg acccgggaaa tcttgtcgga
     4261 acagcacccg atgcgagcta ctggctgctg cgcagcgagg aagcggcgac ggaatatatc
     4321 gtggaagaat attactgggc gtctgcggcg gaatatgccg acagcgtggg agcggatgtg
     4381 atcaactctt cgctcggcta tacggaattc gatgatccgt ccgaaaatca cacctacgcc
     4441 gacatgaacg ggcacacgac accctgcgcg cgtgcggcga acattgcggc gcgcaaagga
     4501 atggcgctcg tcatcgcagc agggaattcg gggcagagtc cgtggtttta tatttccacg
     4561 cccggcgacg ccgacagcgt gctcacggtt gccgcgctgg acgcttccgg caacgtggcc
     4621 ggtttcagct cccgcggacc tgcttccgac ggggatgtta aaccctgcac aggcgcacag
     4681 ggtgtgaatg cggtgatcgc atcgccgacg ggaggaacgc agaccggtaa cggaacttcg
     4741 ttcgccacgc ccatcacctg cggactcgtg gcctgtctct ggcaggcgca tccgtcgatg
     4801 acgaacatgc agctgatcca gtacatcgaa atgagcgcca gccagtattc gaatcccgat
     4861 tcgctgatgg gatacggcat accggatttc tgcgcggcca atctcgcgct ttccccgaat
     4921 cccgtttacg accaggcgaa cgattatctc tacaacgtgg tgccgaatcc ttttgtaaac
     4981 cagctggatt tttcattcta ttcgatgacg gaccaggata tacaggtgaa cctttatgat
     5041 gtgcagggac ggcagatgat cgaccagctc gtgtttgctg cgaagaaagc ggaaaatcat
     5101 tacacgctga cagaacttga agcagcgggt ccgggtgtgt attttcttgt cgtgaaggga
     5161 aacgaaggaa cttactattc ccgtatagta aaaatgtgaa cacgttcccg cattcacatt
     5221 tcacttttca ttttaagaaa ttggctaccg caacccggaa ttgatatccg ccaatgcgga
     5281 agatcccggg cacagctctt tttccgtgag cgtgttcagc ggcttttgcg ttgtattcag
     5341 gatgtcgtga aggtctttcg aattttcatc gatatacagc agcccggtaa ggatctcgcc
     5401 tttgtcctgc gcttttttga tcgcatgtat agctgaaaaa cgatcctgcg gatcccattc
     5461 ctgctcgagc ttgtgcagct gtatggcggt gccgtcgtgc atttcaactt tcttcacggt
     5521 tccttcttcg taggaagtcg tgatctcttt catttcagga acgaaatcca tcgtcgccgt
     5581 ggcttccatg tgttcgcgta cgaaatcata cgacttcgtg gagccgggat tgttgttgaa
     5641 cgtgacgcag ggagaaatga cgttgatgaa cgcaaagcct ttatgcttca tggccgcttt
     5701 gatgagcgga acgagctggc gcttgtcgcc ggaaaagctc tgtgcgacga acgttgctcc
     5761 gagctcgagc gcgaggctgg caagatcgat ggcatgaaaa ggattgacgg agccggcttt
     5821 gcttttcgaa ccttcatcgg ctgtggcgga atcctgtccc ttggtgaggc cgtagcaacc
     5881 gttgttcatc acgatgtacg tcatgttcag gttgcggcgg atcacgtgaa cgaattgtcc
     5941 catgccgatc gaagcagtgt cgccgtcacc ggaaacaccg aaataaataa gatcgcggtt
     6001 gccgaggttg gccccggtgg caacggaggg catgcgcccg tgaacggagt tgaacccgtg
     6061 agaattgctc aggaaatacg ccggcgtttt ggacgagcag ccgatgcctg acatcttcgc
     6121 cactttgtgc ggttcgatgt tgttctcgaa gcaggcctgc acgatggcag cgctgatcga
     6181 gtcgtgaccg cagcctgcgc acagcgtaga gagcgctcct tcataataat cctgcgtgta
     6241 gcccagtttg ttcttgggta attcaggatg gcggaatttc ggacgaacgt atgtcataaa
     6301 atcagcgaac ctctttttgt ttggatgtag tggaaagatg ttttgtgatc tgcttcagga
     6361 tgttgtcggc ggtgatcggc atgccatcgt aattgaggac agcgaccagt ttttccgggt
     6421 tgatgccgag ctccatctgg agcatgctgc gcatctgccc gtcgcggttc tgctcgatca
     6481 cgaaaacacg cttgtgcgcg tggatgaaat cttcaaccgt tttgctgaac gggaatgcga
     6541 gaagacgcat cgcatcgacg tacacgccct gttccttcag caggtccatt gcttcttcgg
     6601 ccgaatacgt ggatgttccg aagaaaacga tggcgttctc gaagctgttc ttgtcctggt
     6661 aaagatgggg tgcaggcacc atttttttcg ccgtttcgaa tttcttcgtg aggcgatcca
     6721 tgttgcgcac ataggccgcg ccgtcctccg tgtacgttgc gtactcgtcg cgtgaagttc
     6781 cgcgcgtgac gaaagcgcct ttcgtgggat gaacgcctgg aatggtacgg tacgtaatcc
     6841 cgtcgttatc gacgtcgagg taacggccgt aacgttcgag cttatccagt tgttccgcgg
     6901 tgaggacttt cccgcgcctg agcttacgct tatcgtccca tttgaacggc tcggaaatat
     6961 gatcgttcat gccgagatcg agatcggtca tcaccatcac gggcgtctgc aattcttcgg
     7021 caagatcgaa cgcttccgcg gtcatctcga aacattcttt cggagtattc gggaaaagaa
     7081 gaacgtgctt tgtatctccg tgcgacgcat aggccgcttc gagaatatcg gattgctgcg
     7141 tcctcgtcgg catgccggtg gaaggacccg tacgctgcac atcgatgagc acagccggaa
     7201 cttccgcgaa gtaggcgaga ccgaggaatt cgttcatcag cgaaacgccg ggaccgctgg
     7261 ttgcggtgaa cgaccgcgcg ccgttccagt tggcgccgat gaccatgccg atggcggcga
     7321 gctcgtcttc cgcctggaca acggcataat tgtgcgtgcc ggtttccttg tctacgcgca
     7381 gctgttccgc gtagccggag aacgcttcag ccaccgaagt ggaaggcgtg atcgggtacc
     7441 atgcgacgac ggtggctcct gcgtatacag cgccgagcgc gcaggcggaa ttaccgtcaa
     7501 ccatgatcga atcgccgaga aggttgcggc gttccacgcg gatgtcaagc gggtagttga
     7561 aattctcttt cacataatcg acgcccattt tcaacgcttt cacattgggc gcgatcagct
     7621 tttctttccc ggcgaactgg tcggcgagaa gatcttccag cactttgaac tcgatgttca
     7681 gcagcgcgga aagcgcgccg acataaacga tgttcttgaa cagctggcgc tggcgggcat
     7741 ccgtaaattc acggttgcac atcgccatca gcggaatgcc gatgtaatta atgtcgcggc
     7801 ggatgaattc ctcgtgcagc gctttcgtgc agtcgtaaag aaaataaccg ccgggacgca
     7861 cggaggcaac atcctgcttc atgctctgcg ggtttacgca aaccatgatg tcaacgccat
     7921 ctctccgccc gagatatcct ttttcattga cgcgcacttc gtaccacgtg ggaagtccct
     7981 ggatgttgga cgggaaaata tttttgggcg ttacaaaaat tcccatccgg aaaatcgcct
     8041 tggtaaaaag gaaattcgca ctggctgacc cggttccgtt cacgttcgcg aacctgatca
     8101 cgaaatcatt caccttctcg ttcatacctg gtatgcttta gttacgctgt agaagaattt
     8161 ctgcatgtcc cacgcagagg tggggcaacg ttccgcgcaa aggccgcaat ggagacagac
     8221 attctcatct ttcaccatta cgcgtttggt gggaagcacg tcggacacgt aaagcgcctg
     8281 cgattccacg ttggcgggcg ctttcagccg cgtccggaga tcgctctcgt cgccgttctc
     8341 cgtgaatgta atgcaggagg tcgggcagat atcgacgcag gcgtcgcatt cgatgcattt
     8401 gttttccgta aagaccgttt ccacgtcgca gttgaggcaa cgttccgctt ctttgaaccc
     8461 ggtcggaaga tcaaagccga gctcgacttc cttcttgcga ttggagagcg tcactttttt
     8521 gtccgcctgc ggaaccatgt atcgcttgtc gacgacaacc atgttgtcgt agctccattc
     8581 gtggatgccc attttctggc tgacgagatt cacgtaagga gatgggcgct tcatcaggtc
     8641 ttcgttgcgg caggcgaggt cgatggaaat tgcggcctgg tgcccgtgcg ccaccgcggt
     8701 gatgacgttc ttcggaccga aagctgcgtc tccgccgaag aatacttttt tattcgtgga
     8761 agcgaacgtt gtttcattca cgacaggcat gtcccattta tcgaattcga tgccgaggtc
     8821 acgctcgatc cacgggaagc tgttttcctg cccgatagcg acgagcacgt cgtccgcttc
     8881 gtggaaaact tcaggctcgc cggtcgggac cagcttgcgt ttcccgtttt cgtaaacagc
     8941 tttcactttt tcaaacatca tccctttgag cttcccgttc tcaataacga agcttttcgg
     9001 aacgtggttg tcgatgatgg gaatatcctc gtgcatcgcg tcttcttttt cccacggcga
     9061 cgctttcatt tctgcgaacg ggctgcggac gatgaccttc acctgctctc cgccgagacg
     9121 gcgggaagtg cggcagcaat ccatcgcggt gtttccacca ccaagcacga tcactttttt
     9181 accgatctta ttcgtgtgct cgaacgcgac gttggcgagc cagttgatgc cgatgtgaat
     9241 attcgcggcg ccttctttcc ttcccgggat gtcgaggtcg cgtcctttcg gagcgcccga
     9301 tccgacaaaa acagcgtcat aatctttggc gagaacctct ttcaggcttt tcacatacgt
     9361 attgaaatgc gtgtgaatgc cgaggtcgag aatataattc acttcctctt cgaggacagt
     9421 ttcgggaaga cggaacgacg ggatctggct gcgcatgaaa ccgccgccgg ctttctgctc
     9481 gtcgtacagg tgaatttcat aaccgagcgg agccaggtcg cgcgcaacgg taagcgatgc
     9541 cgggcctgcg ccgataaggg cgatcttctt tccgttcggt ttgaacggtc cggccggcat
     9601 gtgctgtttt acgtcgcctt tgttgtcggc agctacgcgc ttgagccggc agatagcgac
     9661 aggttcctca ttcggttcca ggcgcccgcg gcgacaggcg ggttcgcagg gacggtcgca
     9721 ggtgcggccg agaataccgg gaaacacatt cgattcccag ttgatcatgt aggcctcggt
     9781 atatttctgc tcggcgatca gccgtatgta ttcgggcaca ggtgtgtgcg ccggacaggc
     9841 atactggcaa tcgaccactt tgtggaaata ttccgggtct tttacattgg ttggtttcac
     9901 agaattcagg tctttttggt tgctatgcag ttgcgttttt tccgtgggga aagccctgta
     9961 aaaatgcgct gcggagcgct ttccggagca gtggtaaaag tatcattttt ttgctttgga
    10021 aatatgcgtg gaaaatgcgc ccgggccccg gaaaattctt tatcgcacat cttcaattcc
    10081 ctgctttccc gcatacaaat tttgctgtaa aaacgacgca tcacgtctca ttttcgacca
    10141 atggataaaa cgcgttgctg aaaaatgcgc cggggcggga aaactcaatg gcgggcgtgg
    10201 ccggaaattt tcttcagaag cggcagaccc gtaatggcag cgatcttttc agccagcctg
    10261 tcgccttccg gtgtgaacgg ccagacgtaa agaaaacgga tgccgtgatc cttccggata
    10321 ttttcaatcg cttcgacggt cagcttccgc gctagtcctt ttttcctgaa ttcgggcagg
    10381 accgttgccg agcagaggta gatcgcttca aatttttccc cgggagaagt gcgcgcaagc
    10441 agctcgtttt ccggtatcgc gccgtcgagg aaaagccgca ttgttttttc ggttgtcggg
    10501 atgacaagta tccataccgc aggaccttca ccgtcgtcgt aatcggaaag cgtggcggga
    10561 tggatttcac ccagcttcct gagaacatcc tcatcgacct ggagctggga agggtcgctg
    10621 cgggtggaaa aaacatcatc caccagctgc agcatt
//
LOCUS       DASTSF010000010        17025 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_2001507,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000010 DASTSF010000000
VERSION     DASTSF010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 17025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17025
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2001507"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            201..455
                     /locus_tag="VFU15_00230"
     CDS             201..455
                     /locus_tag="VFU15_00230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716223.1"
                     /translation="MQQDTWDNKAGNPGIKAYDFGPRAGLFYHFNGKFTAETNFYYGV
                     TNIVKDQELAQYATWKVQQWTMGVRYNILKPAGSSGTSAQ"
     gene            complement(488..1417)
                     /locus_tag="VFU15_00235"
     CDS             complement(488..1417)
                     /locus_tag="VFU15_00235"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HEU4716224.1"
                     /translation="MKKNILLAVLPFVLLLLSAGNDPKLPKLKSATVAINGTQIMIDQ
                     GEVSMADWFTFIYKTAYDEKNDGFNQVRYDSLMPDTNILPQKYRFLVRMFAKLDNFYG
                     NGGTDKWRWFFGDNTYTLGFFIPVSESVWGVDSLKTKIKNYMKLPVVAITYSQAQEYI
                     AWRAQLASAEKKVADDGWKVKGRLMTKEEWQNMATSLGPRFATNNTAQIDTVNTKGCY
                     LLNIRTDKPCESVLEGRKVFGEGAVGVFSYNPDRNGLYNIFGNVAEMTQEEGIAIGGS
                     YADYGIMCNASKTVSYSKPEPWLGFRCVFEFSR"
     gene            1643..5218
                     /locus_tag="VFU15_00240"
     CDS             1643..5218
                     /locus_tag="VFU15_00240"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HEU4716225.1"
                     /translation="MRFLFFFNLLTAIIIYSCSDGSGSPGKAEKEKTFSPPAIIPAGE
                     PSEAPFAPPVQTAAGDPVVRPAGFFIPMQSYNTEEGLALSSVRSGCADHLGNLWFGTD
                     GGGVSRFDGKKFSTFTVSCGLPSNIIMCIREDRSGNMWFGTYGGGVCCYDGTCFRTYN
                     QKSGLAGNIVYSILEDRKGNLWFGTESGGVSRLDPSSANGKSFTNFTTADGLAGNSVW
                     SMMQDRKGRIWFGTGGNGISCYDPDGGKGKLFTNYSAADGLAGNTVYSMLEDHSGNLW
                     FGTRGNGVSCYDGNHFINYSTANGLSSNSVYSLHEDKTGRIWMGTYGGGVDRFDPRAQ
                     EGKAFTIYNIAQGLSGDIVWSILEDKSGNLWFGTYGGGVCRYEGNTVSTYTTEQGLSS
                     NIVLGLLQDRSGNFWFGTEGGGVSLYDGKNFYIYSGAQGLSASSIWYILEDHTGKLWF
                     CTSGGGVCCFDKERKTFSTYTTAQGLASNFIRSAMEDRDGNLWFGTDGNGVCCLHPDA
                     KNGRSFATYTTAQGLANNTVRCMHEDRNGNIWFGTAGGGVSCLRTDGRGGKTFVNYSI
                     LQGLAGNTVLSILEDSCGNLWFGTSGEGVCRFNPAEKSEKKFMIISTAEGMADNSIWS
                     MQMNHEGTIWFGTNLGFSGLTGFREKSSGKIVPSSCAISNAELAEKYIPVFDVFNQKT
                     GYPVKDANTNALICDNAGVMWEGTGDKLVRFDHTQVHRDFLPPEVLLQAVKIKDENIC
                     WYDLSDKNPPVYDSLVSSPAFTEEAVTTGQALPPAVRDSMRSRFAGVRFDSISRFCYL
                     PQHLVLPHEHNSITFDFTAIAPARPFLVRYRYFLDGYDKTWSPPTDMTTATFGNIFEG
                     TYTFNVQAQYIGGTWSAPVTYTFTVLPPWYRTWWSYLSAFLLFTGGLWLFIRWRERSL
                     KREKDILEEKVEVRTQQLREQKQIVEVKQQEILDSLNYARRIQYTLLAHDDLLNANLP
                     EHFILFRPKDIVSGDFYWATVSGSGDDRRFYLAVCDCTGHGVPGAFMSLLNISFLNEA
                     ITEKNITGPDKILGYVRERLLGSVSKDGAQDGMDGILLCIEKNGITYAAAHNAPVIVR
                     NGELTEFPADKMPVGKGELNNPFTLHRVDVQKGDMLFLYTDGFADQFGGPKGKKIKYR
                     KLHSILSGNAALSLKLQAEKLEKEFDDWKGTLEQVDDVCVIGVRF"
     gene            5423..6574
                     /locus_tag="VFU15_00245"
     CDS             5423..6574
                     /locus_tag="VFU15_00245"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
                     /protein_id="HEU4716226.1"
                     /translation="MDRADALNERAFELSQKNNYDAGAATALHNKGLVHLNKGEFPEA
                     VSFFSRSIELKEKIRDMAGIIASNINLGIVFYHLRQFEKSLAFSTEALELARKEKYRQ
                     LEMKTLANIAMVHGETGNIETALDHYLLTLTIAGQLGETRTAALLKDNLGVLYRMKGE
                     YEKALAYHRESLKEKKVFRDYPNIARSMGNIATVFLQQKKFREAITWFKKALKVSLDI
                     DAKDITKMLYNNIAECYARMKKFDQAYRYKLYYDEFHESVMSDASRQQVAEIEAKYQA
                     QKKEREIEKLRSALKEKEDIINTILKKNERVMLPESFSSLSKKEIETLSYVSRGMTDK
                     EISAGMNVSVNTVKTFLRRVFSKLSVNNRTELVALIHRYDFSAEAGKKN"
     gene            complement(6718..8115)
                     /locus_tag="VFU15_00250"
     CDS             complement(6718..8115)
                     /locus_tag="VFU15_00250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716227.1"
                     /translation="MNLTKDRYRLFTGLFFLAMTIFAIVFAGERFQADGAYYLFHVVD
                     SGNFRIEHQRYILAVSQALPLLGVKLGLSMHAVILLNSLNNIVFFFLVFLYAVRYLHD
                     RTAGIAVILFQVLGVMHIQFIPMYEVWYGSVLLVLVRSHLLHNRYRSIPDFVLLTAIM
                     VTVLFSHPLLFIPLIFIILLDAIEKRKISWKLLFTYAVIFSGWYIIKKLFLSEYEAGK
                     LSLLDFGWNKAYRNLFHFGYLWGLVVFFFKYYTIPVLAFIFMMIVYIVRRAKMKAFLL
                     AAFFFGIILLINITNENIPEISPYFERMYMPLIPISLLPFLYDFFAQFGWKSTAGGIL
                     VMLVVTWRIYLFCSVGMMYSERTAITERVIHQAQAMGGSKFELAESDYRGCMSWVDWS
                     YPMETFLRSACDGKDKTVTVLTSEDLDEGRNRQMMNDRLFFLRRWDPRADYFINKNYF
                     VVKDGPYKKLQPECR"
     gene            complement(8136..9143)
                     /locus_tag="VFU15_00255"
     CDS             complement(8136..9143)
                     /locus_tag="VFU15_00255"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012780832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEU4716228.1"
                     /translation="MSYRIGVIGGGSWATAIIKMLCNNVTGSDAGKINWWLRNPETIA
                     YIRKYRHNPNYLSSVELHLDQLVLSDDIAQVVSNSDILIVAVPSAFLADSLSGLAKND
                     FSGKIVFSAVKGIVPGPNLIVGEYFNTEFGIPYTNIGVITGPCHAEEVALEKLSYLTV
                     ASQSNEYAQFVAQKIACRYIRAGVSDDIYGTEYAAVLKNIFAVASGICHGLGYGDNFQ
                     AVLISNAIQEISRFVDAVHPIDRDIKSTAYLGDLLVTAYSQFSRNRMFGAMIGKGYSV
                     KMAQLEMNMVAEGYYAAKCIHEINGRYGVSMPITDAVYNILYEKISPVIEIKLLTDKL
                     T"
     gene            9497..11359
                     /locus_tag="VFU15_00260"
     CDS             9497..11359
                     /locus_tag="VFU15_00260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716229.1"
                     /translation="MRKVVNRISFYTLLAISCIFVYVEIREIRNNDRLISGSGPISWD
                     SYGYYLHLPATVIHHDPGITDRSWLDSLNAKYQQGRPWYQALPGVKGRMVNVYTVGPP
                     VVWLPFFLAGHACAKLFGYPTDGLSPPYQYAMIIAGLFYALLGLTLLRKLLLKYVNDR
                     ITAIVLAVIALGTNLYHYSVYSNTMPHILIFPFGVMIILLTISWHENPSKLKALGLGA
                     LVGLVTIIRPSELVWILVPVFWNVVSIKTAFAKIGFAFRHFTHVLIAGITLGCVLMLQ
                     MAYWKFTVNHWFSNNHSEGFDLFHPFVWQTLFSYKKGWLLYTPVMLFAIAGFWTLFRK
                     KRELFVPLFIFFLANVYAISAWECWWYAGSFGQRPFVSSYGLMAVPLAFFLQEISVMR
                     VMKWVAGFFIAAFSFLNQFQNWQLEQSILHYELMTKTYYWKIFGRTQDDPSLKKYLEI
                     DRHALPPLEQVKDDYIEKTIYFSNYENYTPVNGELVCDTFGLSGTHSLCLDADHIYGV
                     MVKMPYNKITDKDHLRIKMEADVFIPPGSEDGNLNFTGCMTSDREKDYGYVSFSAQSL
                     GATPGSWTHVTAWYVTPFILHRSDVLAIVVWNQGGKKVFLDNMKTTAYEPKVSY"
     gene            complement(11517..>17025)
                     /locus_tag="VFU15_00265"
     CDS             complement(11517..>17025)
                     /locus_tag="VFU15_00265"
                     /inference="COORDINATES: protein motif:HMM:NF036531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HEU4716230.1"
                     /translation="NPDYFSPAVIFTTLPLNSPDTMSMDFYALRTLQTLEKMHFPEKD
                     PDALVDVELERLLFVHNKSVDPDKDSLYEMGLRQLAANYPDSKITAEVLYQLAVYHNS
                     LGAQYVPGKDEKYRWERKRALDICNAVIQKYPGTPGAYQCSIIRNDITAKNVSFTVQD
                     ASVPGKPSPGLLTYRNFSHVYFRIASVPQEYVAKTDYRTTEQVIKDYLALPVLQSWDL
                     SLPADSDYADHSVEIKIPDLPAGKYVILSSVDKDFTMMKNAIAYTEMWSTTLGYVQRQ
                     LTDGSFDFLVFDRENGGPLEGVSAQVWYQSYDYVTRDYRYKKGDRYFSDKDGRVNVPP
                     AGNYRTFFLELSSGGNHFITGSSYQYKPYNAPREMRPQTFFFTDRSIYRPGQTIYFKG
                     IIVNTDGDNNKILPHKTTTVTFYDVNYQKVTSLNLETNDYGSFSGTFTAPSAGLTGQM
                     HIQNESGSEYFSVEEYKRPKFDITTQPVKGAYRLNDSVFVKGSARTFAGSAVDGAKVT
                     YTVTRSASFPWWYYYWWGYYPNSQTAVISNGEVMTNDTGGFVIPFKALPDKSVPKSSS
                     PTFNYTINIDVTDITGETHSTSAYVNVGYVALELSVNADNYIEKSDTAGIPVSLTNLS
                     GEPESSMVSVSIHRLNDPGKIYKDRQWQVPDKFIYTKDDWTTTFPDLPYKEENKQVNW
                     TKGSEVYSGKYNTGKEKSLRLKNKDSWKPGSYIIEATAKDKYGQEVKDVKYFTLYDIR
                     DKMPASKEAWSFQAINTNCQPGDKAVFLVGTSGKNVKVLYETEIQGKIASRQWLTLNE
                     EQKYIEIPVEEKHRGNFAVHFAMVYHNQAYTSDAGVSVPWESKQLDFEYETFRNKLIP
                     GQKEEWKIKIKGPKGDKVAAEMVAAMYDASLDQFRSHNWSFYLYKSYYGSLYWQHGVE
                     GTATSALYAPYWNDNSLPDYQYRTYDQLNWFGFYFSYGNYWYGYDGNGPGYLREEMDE
                     DNLGVSGGVVDIAQSKAVDKSKAPAAPPPADAATGSPTFAFSTSGNTAHASGEKNTET
                     TALMQDSPAAGKDYRNAPNLNAIAARQNLNETVFFFPQLETDSTGTVIIKFTMNEALT
                     KWKFMAFAHTKDLMYGQTTKEVVTQKDLMVMPNAPRFFREGDKITFTAKVSNLTDKDM
                     TGDAQLMLFDALTMQPVDAKFLNNNAKLSFTAKKGQSAPLSWDLIIPYDVSAVTYRVV
                     AQSGNFTDGEESSLPVLSNRMLVTETMPIWVKQGETKEFRFEKLLSMNGGSTTLRNQR
                     LTLEFTSNPAWYAVQALPYMMEYPYECSEQTFDRLYSNSIASNIANSSPKIRAVFDAW
                     KTQSPDAFLSNLEKNQDLKNVVLTETPWVLDAKDETERKRRVGLLFDMNRMADEQDRT
                     IRKLRQMQSPNGGWPWFEDMPDDRYITQYIVTGFGHLDKLGVKSVREDNRNWNMITSA
                     MQYLDNRVREDYDWILKYDKDHMNEDHLSDIAIQYLYARSFFPEIPMTPRNKTAFDYY
                     YGQSQKYWLGKGNFMEGMIALELNRYNDKKTSGDIMKSLTETALHSDEMGMYWKDNSG
                     GYYWYQAPIETQSLLIEAFKEVKNDQKSVDDMRIWLLKNKQTNDWKTTVATADACYAL
                     LIDGTSWLATESNVEIVVGSQKIDPKKLDVPEEAGTGYFKTAWSGDAITPDMGKVTVT
                     KTGPGISWGAMYWQYFEDMDKITPAQTPLSVSKKLFLVKKTSSGEVMEPVTDQTVLHP
                     GDKIRVRVELRNDRDMEYIHMKDMRAAGFEPVNVLSQYKWQDGLGYYESTKDASTDFF
                     FSFLPKGTHVFEYPLTVVNAGDFSNGITEVQCMYAPEFSCHSEGIRVKVAK"
BASE COUNT         3955 a         4486 c         4531 g         4053 t
ORIGIN      
        1 aaaccggcgg cggacgtgaa tccggcagcg atgcgtggaa agcgtacatg cgccgccaga
       61 caaacacgat caattacggt tcgcagctcc cgctcgcgca gggcatcgag ttcactatct
      121 gataaacgca caattcattc acgaagagcg ttcccgcaag gagcgctctt tttcgtaatc
      181 tgaaattcac aactccaaac atgcagcaag atacgtggga caacaaagca ggcaatcccg
      241 gcattaaggc gtacgatttc ggtccgcggg ccgggctgtt ctaccatttc aacggcaagt
      301 tcaccgcgga gaccaacttt tattacggcg tgacgaacat tgtcaaagac caggagctgg
      361 cgcagtatgc gacgtggaaa gttcagcagt ggaccatggg tgtacggtac aatatcctga
      421 agccggccgg aagttcagga acatctgcac aatagaattg cgcagaaaga cagccctgcg
      481 gtctttctca gcggctgaat tcaaacacgc agcggaaccc gagccagggt tcgggctttg
      541 aatacgaaac cgttttcgac gcgttgcaca tgatgccgta atcggcatag ctgccgccga
      601 tggcaattcc ttcctcctgc gtcatttccg caacattccc gaaaatattg tagagcccgt
      661 tacgatcggg gttataggaa aaaacgccga ccgcgccttc cccgaaaact tttcttccct
      721 cgagaacgct ctcgcacggt ttgtctgtcc tgatattcag caggtagcat cctttggtat
      781 ttaccgtatc gatctgcgcc gtattattcg tcgcgaagcg cggtccgagg ctggttgcca
      841 tattttgcca ttcttctttc gtcatcagcc ggcccttcac tttccatccg tcatcggcca
      901 ctttcttttc cgctgaagcg agctgcgctc tccacgcgat atattcctgt gcctggctgt
      961 acgtaatggc cacaaccggc agcttcatgt aatttttgat tttcgtcttc aggctgtcca
     1021 cgccccacac actttcggaa acaggaatga aaaaacccag cgtgtatgta ttgtcaccga
     1081 aaaaccacct ccacttatcc gttcccccgt tgccgtaaaa attgtccagc ttcgcgaaca
     1141 tgcggacgag aaaacgatat ttctgcggaa ggatattcgt atccggcatc agcgagtcgt
     1201 agcgcacctg gttgaacccg tcgttttttt cgtcgtaagc ggttttgtaa atgaacgtga
     1261 accagtcggc catggaaact tctccctggt cgatcatgat ctgtgttccg ttaatcgcaa
     1321 cggtagcgct tttcagcttc ggcagtttgg ggtcgttgcc ggcagacaac agcaggagaa
     1381 caaacggcaa gacagcaagg aggatgtttt ttttcatagc aggacgttgg gtaggccgcg
     1441 ggattacaca ccggacctgt ttgttatgga gcaaatatgc caaacatttc cgtttgttct
     1501 tatactactt aagtgctatt ctacggaaga acgcaagggt ttaatttgca tcgcgaattg
     1561 cggagatccg ccgccggccg gcatttgcaa ccgcatgcgc gaatacgtta tcattgaaat
     1621 acttttattt ttagtccccg atatgcgttt cctgtttttt ttcaatttat taaccgcgat
     1681 tatcatttac tcgtgcagcg atggcagtgg ctcgcccggt aaagcagaaa aagagaaaac
     1741 cttttctccg ccggcgatca ttcctgccgg cgaaccttcg gaagcgccgt tcgctccgcc
     1801 tgtgcagacc gcagcgggag atccggttgt gcgacccgcc gggtttttca ttcccatgca
     1861 aagctacaac accgaagagg ggctggcgct cagctcggtc cgcagcggtt gtgccgatca
     1921 cctgggaaat ctctggttcg gaacggacgg aggcggcgta agccggtttg acgggaagaa
     1981 attttccacg ttcacggttt cctgcggact tccgagcaac atcatcatgt gcatacggga
     2041 agaccgctcg ggcaacatgt ggtttggaac gtatggcggc ggcgtgtgct gctacgacgg
     2101 aacctgtttc cgtacctaca accagaaatc gggactggca ggcaatattg tttattcgat
     2161 cctcgaagac cgcaaaggca acctctggtt cggaacggaa agcggcgggg tcagccgtct
     2221 cgatccttcc tccgccaatg gaaaatcatt tacaaacttc acgactgcag acggactggc
     2281 aggaaacagc gtgtggagca tgatgcaaga ccggaagggg cgcatctggt tcggaaccgg
     2341 ggggaatggc ataagctgtt atgatccgga tgggggaaaa gggaaactgt tcacgaatta
     2401 ttccgcagcg gacggtcttg ccggcaacac ggtttattcc atgctggaag atcattccgg
     2461 aaatctctgg ttcggaacga gaggaaatgg cgtcagctgc tacgacggaa atcatttcat
     2521 caattattcc acggcgaacg gactttcctc gaacagcgtt tacagtcttc atgaagacaa
     2581 gaccggcagg atctggatgg gcacttacgg cggcggcgtg gatcgtttcg atccgcgcgc
     2641 gcaggaagga aaagcattta ccatctacaa tattgcgcag ggcctgagcg gcgatattgt
     2701 ctggagcatt ctcgaagaca aatcgggaaa cctgtggttc ggaacgtatg gcggaggcgt
     2761 ctgccgttat gaaggaaata cggtatccac gtacaccacg gagcagggac tctcgtcgaa
     2821 tatcgttctt ggcctgctgc aggacaggag cggaaatttc tggttcggaa cagaaggcgg
     2881 cggagtatcc ctgtatgacg ggaaaaattt ttacatctat tccggcgcgc agggactttc
     2941 cgccagcagc atctggtaca ttcttgaaga tcataccggg aaactctggt tctgcacttc
     3001 aggcggcggt gtgtgctgtt tcgataaaga gcggaaaaca ttcagcacat ataccacggc
     3061 gcaaggactg gcaagcaatt tcatacggag cgcgatggaa gaccgtgacg gcaacctgtg
     3121 gttcggaaca gacgggaacg gcgtgtgctg tctccatccc gatgcgaaaa acgggcgatc
     3181 atttgccacc tacacgacgg cgcaggggct tgcgaacaac accgtgcgct gcatgcacga
     3241 ggacaggaac ggaaacatct ggttcgggac agccggcggc ggagtgagct gcctgcgcac
     3301 ggacggaaga ggtggaaaaa cattcgtcaa ttattccatc ctgcagggac ttgccgggaa
     3361 taccgtactg tcgatcctgg aggacagctg cggcaattta tggttcggca cttccggcga
     3421 aggagtgtgc cgtttcaacc cggcggaaaa aagtgaaaaa aaattcatga tcatttccac
     3481 cgccgaagga atggccgaca acagcatctg gagcatgcag atgaaccatg aaggaacgat
     3541 ctggttcggg acgaaccttg gcttcagcgg cctgacaggt ttccgcgaaa agtcctcggg
     3601 aaaaattgtc ccgtcttcat gcgcgatcag taatgcagag cttgctgaaa agtacattcc
     3661 cgtttttgac gtgttcaacc agaaaaccgg ttacccggtg aaagacgcga acacgaatgc
     3721 gctgatctgc gacaacgcag gtgtcatgtg ggaaggaacg ggagacaagc ttgtccgctt
     3781 cgatcatacg caggtccacc gtgattttct tccgccggaa gttcttctcc aggctgtaaa
     3841 gatcaaggat gaaaatatct gctggtatga tctttcggat aaaaatccgc cggtgtacga
     3901 cagtctcgtt tcttctccgg ccttcaccga agaggcggtc accacaggac aggcgctgcc
     3961 gcccgccgtc cgcgacagca tgcgcagccg cttcgccggt gttcgtttcg acagcatttc
     4021 acggttctgc tacctgccgc agcatcttgt tttaccccat gagcataaca gcatcacgtt
     4081 tgattttacc gccatcgcac cggcgaggcc tttcctggtg aggtaccggt atttcctcga
     4141 cggctatgat aaaacatgga gcccgccaac agacatgacg accgccacct tcggcaacat
     4201 cttcgaagga acgtacacat tcaacgtgca ggcgcaatac atcggcggaa catggagcgc
     4261 accggtcact tatacattca cagtcctgcc tccgtggtac agaacgtggt ggtcttatct
     4321 ttccgcgttc ctccttttca cgggcggatt gtggttgttc atccgctggc gggaacgatc
     4381 gctgaaacgg gagaaggata ttcttgaaga aaaagtggaa gtgcggacac agcagctacg
     4441 cgaacaaaaa cagatcgtcg aagtcaagca gcaggaaatt ctcgattcgc tgaattacgc
     4501 ccggaggatc caatacactt tgctcgcgca cgacgatctg ctgaatgcaa acctgccgga
     4561 acattttatc ctgttccgtc ccaaagacat tgtgagcggt gatttttact gggccaccgt
     4621 ttccggctcg ggcgacgatc gccgttttta ccttgccgtg tgcgattgca cgggacatgg
     4681 cgtccccggc gcattcatgt ccctgctcaa catctctttt ctcaacgaag cgatcactga
     4741 aaaaaacatc accgggcccg acaaaatact cggctatgta cgagaacgcc tgctcggttc
     4801 cgtgtcgaaa gatggcgccc aggacggaat ggacggcatc ctgctctgca ttgaaaagaa
     4861 cgggatcacc tacgcggctg cacacaacgc cccggtgatc gtgcggaatg gggagctcac
     4921 tgaatttccc gcagataaaa tgccggtggg aaaaggcgag ctgaacaatc ctttcacact
     4981 tcaccgggtt gatgtgcaga agggcgatat gcttttcctg tacaccgacg gtttcgcaga
     5041 ccagttcggc ggacccaaag gaaaaaaaat aaaataccgg aagctgcaca gcatcctgtc
     5101 gggcaatgcc gcgttatcgc tgaaactcca ggcggaaaag ctggaaaaag aattcgacga
     5161 ctggaaaggt acgctcgagc aggtagacga tgtgtgcgtg atcggcgtca ggttctgata
     5221 ccagccggcg catggcgtat accgggataa ttaatcacgg gccggcgctc tgccagtcct
     5281 gtttgttttg ccctttcatt ttttctccgg tatttttaca atgcccgaac ccgcagggcg
     5341 cattacgatg aataaacccg gaacagaatt cccggagatc gattctttgc tggagtcgtt
     5401 cagagaacac ctgtactctg atatggatcg tgcagacgcg ctgaacgaac gcgcgtttga
     5461 actttcgcaa aagaacaact acgatgcggg agctgcgacc gcgctgcaca acaaggggct
     5521 cgtccatctc aacaaaggcg aattccctga agccgtttcc ttcttctccc gttccatcga
     5581 gctgaaagaa aaaatacgcg acatggccgg catcatcgca tcgaacatca acctcggcat
     5641 tgtgttctat catttgcggc agttcgaaaa atcgctggcc ttctccacgg aagcgcttga
     5701 gctcgcccga aaagaaaagt accggcagct ggaaatgaaa acgctggcga acatcgcgat
     5761 ggtccatggt gaaacgggga acatcgaaac tgcgctggac cattacctgc ttacgctgac
     5821 gatcgccggg cagcttgggg aaacccggac ggcggcactg ctgaaagaca acctcggcgt
     5881 gctgtaccgg atgaaaggcg aatatgaaaa agcgctggcg tatcaccgcg aatcgctgaa
     5941 agaaaaaaaa gttttccgcg attatccgaa catcgcacgc tcgatgggca acatcgccac
     6001 cgttttcctg cagcagaaga aattccggga agcgataacc tggttcaaaa aagcgctgaa
     6061 agtctcgctc gacatcgacg cgaaagacat cacgaaaatg ctgtacaaca acattgcgga
     6121 atgctatgcg aggatgaaaa aattcgacca ggcataccgc tacaaattat attacgatga
     6181 attccatgaa tccgtgatga gcgacgcgag ccgccagcag gtggcggaga tcgaagcgaa
     6241 ataccaggcg cagaaaaaag agcgggagat cgagaagctg cgttccgcgc tgaaagaaaa
     6301 agaagacatc atcaacacca tcctgaaaaa aaatgaacgc gtgatgctgc ctgaaagctt
     6361 ttcttcgctc tcaaaaaaag agatcgagac gctttcgtac gtttcgcggg gtatgacgga
     6421 caaggaaatt tccgcaggca tgaacgtttc ggtgaacacg gtaaaaacgt ttttacgccg
     6481 cgtattctcg aagctgtcgg tcaacaaccg gacggagctg gtggcgctta ttcaccggta
     6541 cgatttcagc gctgaagccg ggaaaaaaaa ctgatgtgat taccttacac ccggccactc
     6601 gtcgggcagc agcagggaaa accggaagct tgtcacgcac ctggcaggag ttgatggtga
     6661 tgaaatcaaa ataagctacc cccggaatac attttcgtgc acgtgccctt acaaaactca
     6721 gcggcactcc ggctgcagct ttttgtacgg accgtccttc accacaaaat agttcttgtt
     6781 gatgaaataa tctgcgcgcg ggtcccagcg gcggagaaag aacagcctgt cgttcatcat
     6841 ctgccggttc cttccttcat cgagatcttc ggatgtaagc acggttacgg ttttgtcttt
     6901 cccgtcgcag gccgaacgca ggaacgtttc catcgggtac gaccagtcga cccagctcat
     6961 gcagccccgg tagtcgcttt ccgcaagctc gaatttcgat ccgcccatcg cctgcgcctg
     7021 gtgaatgact cgttcggtga tcgcggtgcg ttcgctgtac atcattccta cggaacagaa
     7081 aagataaatg cgccacgtca ccacgagcat cacgagaatt ccgcccgccg tgctcttcca
     7141 tccgaactgc gcgaagaaat catacaggaa cgggaggagc gagatcggta tcagcggcat
     7201 gtacatgcgc tcgaaatacg gtgatatctc cggtatgttt tcattggtga tgttgatcag
     7261 caggataatc ccgaaaaaga acgcggccag caggaacgct ttcattttcg ccctgcgcac
     7321 aatgtaaacg atcatcatga agatgaacgc gagaaccggt atcgtgtagt atttgaaaaa
     7381 gaaaacgaca agtccccaca aataaccgaa gtgaaaaagg ttccggtatg ctttattcca
     7441 cccgaaatcg agcagtgaaa gcttcccggc ttcgtattcg ctgagaaaca attttttgat
     7501 gatgtaccat cccgaaaaaa taacggcgta cgtgaacagc agcttccagc tgatcttccg
     7561 tttttctatt gcatcaagaa gaatgatgaa aatgagcggg atgaacagca gcgggtgact
     7621 gaacagcacc gtgaccatga tggcagtgag caaaacaaaa tcaggtatgc tccggtaccg
     7681 gttgtgaaga agatgcgacc gcacgagcac gagcaacacg gaaccgtacc acacttcgta
     7741 catgggaatg aactggatgt gcatcacgcc gagcacctgg aaaagaataa cggcgatgcc
     7801 cgcggtacga tcgtggagat agcgaacggc gtaaaggaaa accagaaaga aaaaaacgat
     7861 gttgttcagc gaattgagca ggatcaccgc gtgcatggaa agaccgagct tcacgccgag
     7921 caacggcagc gcctgcgaaa ccgcaagaat ataccgctgg tgctcgatgc ggaaattccc
     7981 cgagtcgacg acgtgaaaaa gataatacgc gccgtctgcc tggaaacgtt cgcctgcaaa
     8041 tacgatcgcg aagatcgtca tcgcgaggaa gaacaacccg gtaaacagcc tgtagcggtc
     8101 tttggtaagg ttcatcaggg aacggaactt gtcggttacg tcagcttgtc ggtgagcagc
     8161 ttgatctcga tgacgggtga aattttttcg tagagaatat tgtacaccgc atccgtgatg
     8221 ggcatgctca cgccgtagcg gccgtttatt tcatgaatgc atttcgccgc gtaatagcct
     8281 tccgcaacca tgttcatctc gagctgcgcc atcttcacgg aataaccttt cccgatcatc
     8341 gcaccgaaca tgcggttgcg gctgaactgt gaatacgccg tcacgagcag gtcgcccagg
     8401 taagccgtgc ttttgatgtc gcggtcgatc gggtgaacgg cgtccacaaa gcggctgatc
     8461 tcctggatcg catttgaaat aagcaccgcc tggaaattgt cgccgtaacc gagcccgtgg
     8521 caaattccgc tggcgacggc gaaaatattt ttcagcacgg cggcgtattc cgttccgtaa
     8581 atatcatcgg aaacaccggc gcggatatag cggcacgcga tcttctgcgc aacaaactgc
     8641 gcatattcat tcgactgcga cgccacggtg aggtaggaaa gcttttccag cgcgacttcc
     8701 tccgcgtgac agggcccggt gataacgccg atgttcgtat acggaatgcc gaactccgtg
     8761 ttgaaatatt cacccacgat gaggttcggt ccgggcacga tgcctttcac cgcggagaaa
     8821 acgatcttgc cgctgaaatc gttctttgca agaccggaaa gtgaatcggc gagaaatgcc
     8881 gagggaacgg cgacaataag gatatctgaa ttgcttacga cctgcgcgat gtcatctgaa
     8941 agcacaagct gatcgaggtg aagctcgacc gagctgaggt aattcgggtt gtgccggtac
     9001 ttgcggatgt atgcgatcgt ttcgggattg cgcagccacc agttgatctt gcccgcatcg
     9061 ctgcccgtga cgttgttgca aagcatcttg atgatcgccg tggcccagct tccgccaccg
     9121 atgacgccaa tcctgtaact catagaacac gaaaatacgc ctaaatcgtg tcattggtaa
     9181 atccgtttcg ctctaccttt accgtgatga cccctttgcg gaatgttttc attttgacgg
     9241 tgctgaacta tccgaaagac attccgcgga gcgggatcgg aaaaattatt ccccgaaaac
     9301 acggcattac cactcgtccg cgcgcgggga agtgctgaag catttccccg cgatgtaaca
     9361 catttacaaa acaggtttct tccgcggaaa atccgatgcg gacgcccgca ggaatcttca
     9421 gaatgaattg aagccgctgt agccggggaa agaaacgccg gtaaccgaag aaaagaatag
     9481 tcctattttt gaacccatgc gcaaggtggt caaccgcatt tcgttttaca cgcttcttgc
     9541 gatcagctgc atttttgttt acgtcgagat ccgcgagatc cgcaacaacg accggctgat
     9601 ttccggctcg gggcccatca gctgggattc ctacggatac tacctgcatc ttcccgctac
     9661 ggtcatccac catgatcccg gcatcacgga ccgttcctgg ctcgattcac tgaacgcaaa
     9721 ataccagcag gggcggccgt ggtaccaggc gctcccgggc gtgaaaggaa gaatggtgaa
     9781 cgtgtatacg gtcggtccgc cggtagtgtg gcttcctttt ttccttgcgg gtcacgcgtg
     9841 cgcaaaactc ttcggctacc ccaccgacgg gttatcaccg ccttaccagt atgcgatgat
     9901 catcgcggga ctcttttatg cccttctcgg tctcacgctg ctgcggaaac tgttgctgaa
     9961 atacgtgaac gaccgcatca ccgcgatcgt gctggccgtg atcgcgctcg gaacgaatct
    10021 ctatcattat tccgtttact cgaacacgat gccgcacatc ctcattttcc ccttcggcgt
    10081 catgatcatt ttgctgacga tctcgtggca tgaaaatccg tcgaagctga aagcgcttgg
    10141 tcttggcgca ctggtcgggc tcgtgacgat catacgtcct tcggagctcg tgtggatact
    10201 tgttcccgtt ttctggaacg tcgtcagcat aaaaacggcg tttgcgaaga tcggctttgc
    10261 cttccgtcat tttacgcacg tgctcattgc gggaataacg ctcggctgcg tgctgatgct
    10321 gcagatggcc tactggaaat tcaccgtgaa tcactggttc tcgaacaacc attcggaagg
    10381 cttcgatctt tttcacccgt tcgtgtggca aacgctgttc agctacaaaa aaggatggct
    10441 gctgtacacg ccggtcatgc tttttgcgat cgcgggattc tggacgctgt tccggaagaa
    10501 aagagaactg ttcgtcccgc tttttatttt ctttctcgcg aacgtgtatg ccatctcggc
    10561 ctgggaatgc tggtggtacg ccggttcgtt cggtcagcgg cccttcgttt catcgtatgg
    10621 actgatggcg gttccgcttg catttttcct gcaggagatt tccgttatgc gcgtgatgaa
    10681 atgggtcgcc gggtttttca ttgccgcgtt ctcattcctg aaccagttcc agaactggca
    10741 gctggagcag agcatcctgc actatgagct gatgacgaaa acatattact ggaaaatttt
    10801 cggacggacg caggatgatc cttcgctgaa aaaatacctc gagatcgacc ggcacgcgct
    10861 tcccccgctc gaacaggtga aggatgatta catagaaaaa accatctact tcagcaatta
    10921 cgaaaactac acacccgtaa acggagaatt ggtctgcgac acgttcgggc tttccggaac
    10981 gcattcgctt tgcctcgatg cggatcacat ttacggcgta atggtgaaaa tgccgtacaa
    11041 taaaataacg gacaaagatc acctgcggat aaaaatggaa gcggatgtgt ttattccgcc
    11101 cggatcggaa gacgggaatt tgaatttcac cgggtgcatg accagcgacc gggaaaaaga
    11161 ttacgggtac gtttcttttt cggcgcaatc gctgggcgca acgcccggaa gctggacaca
    11221 tgtgacggcg tggtacgtca ctccttttat ccttcaccgc agcgatgtcc tcgcgatcgt
    11281 ggtctggaac cagggcggga aaaaagtttt cctggataac atgaaaacga cggcatacga
    11341 gccgaaggtt tcgtattgag ctttacccac gacgcgcaca aaggacgttc accgggcgaa
    11401 acatttttgg cggctttgcg caggatgagg atacacagag atccgcggcg ggaaaatttt
    11461 atacgcacaa aaaatcccgg caggttttgc cgggatttgg tttttcatcc ggaatttcac
    11521 ttcgccactt tcacgcgaat gccttcgctg tggcaggaga attccggcgc gtacatacat
    11581 tgcacttccg tgatgccgtt cgagaagtcg ccggcgttca ccaccgtgag cgggtattcg
    11641 aagacgtgcg ttcctttggg caggaagctg aagaagaaat ccgtcgatgc atctttcgtg
    11701 ctttcgtaat agccgagacc atcctgccat ttgtactgcg agagcacgtt caccggctcg
    11761 aaacccgcgg cacgcatgtc cttcatgtga atatattcca tatcacggtc gttgcgcaat
    11821 tccacgcgca cacggatctt atctcccggg tgcagcacgg tctggtcggt gacgggttcc
    11881 atcacttctc ccgatgacgt tttcttcacg aggaaaagtt ttttcgaaac gctcagcggt
    11941 gtctgcgccg gcgtgatctt gtccatgtcc tcgaaatact gccagtacat cgcgccccag
    12001 gaaatgccgg gacctgtttt cgtcacggtc acctttccca tgtcgggcgt gatcgcatca
    12061 ccgctccacg cggttttgaa ataacccgtt ccggcttctt ccggaacatc gagctttttc
    12121 ggatcgatct tctgcgaacc gacgacgatc tcgacgttag attctgtcgc cagccagctc
    12181 gtgccgtcga tgagcaatgc gtaacaggcg tccgctgtgg ccacggtcgt tttccagtcg
    12241 ttcgtctgct tgttcttcag cagccagatg cgcatgtcgt cgacggattt ctgatcgttc
    12301 ttcacttcct tgaacgcttc gatcagcaat gactgcgttt cgatcggcgc ctggtaccag
    12361 taatagccgc cgctgttgtc tttccagtac attcccatct cgtcgctgtg cagcgccgtt
    12421 tccgtgagcg acttcatgat gtcgcctgac gttttcttgt cgttgtagcg gttgagctcg
    12481 agcgcgatca ttccctccat gaaatttccc ttgcccagcc agtatttctg cgactggcca
    12541 tagtaataat cgaacgccgt tttattcctc ggtgtcatgg gaatttcagg gaagaagctg
    12601 cgcgcataga gatactggat cgcgatatcg ctgagatgat cttcgttcat gtggtccttg
    12661 tcgtatttca ggatccagtc gtaatcttca cgcacgcggt tgtcgaggta ctgcatcgca
    12721 gaggtgatca tgttccagtt gcggttgtcc tcgcgcacgc tcttcacgcc cagcttgtcg
    12781 aggtgaccga aaccggttac gatgtattgc gtgatgtaac ggtcgtccgg catatcctcg
    12841 aaccagggcc acccgccgtt cggcgactgc atctggcgca gcttgcggat cgtgcggtcc
    12901 tgctcgtcgg ccatgcggtt catgtcgaac aataagccga cgcggcgttt gcgttccgtt
    12961 tcatctttcg cgtcgagcac ccagggcgtt tccgtgagca cgacattctt gaggtcctgg
    13021 ttcttctcga ggttcgagag gaatgcgtcc gggctctgcg ttttccacgc atcgaacaca
    13081 gcgcggatct tcggggaaga attcgcgatg ttcgatgcaa tgctgttcga gtacaaacgg
    13141 tcgaacgttt gctccgagca ttcgtacgga tattccatca tgtacgggag cgcctgcacg
    13201 gcgtaccacg cgggattcga tgtgaactcg agcgtgaggc gctggttgcg cagcgtggtg
    13261 gatccgccgt tcatgctcag cagtttttcg aaacggaatt ctttcgtttc gccctgcttc
    13321 acccagatcg gcatggtttc cgtcacgagc atccggttgg aaagaacggg cagcgacgat
    13381 tcttcgccgt cagtgaaatt tcccgattgc gcaacgacac gataagtaac cgcgcttaca
    13441 tcatacggga tgatcaaatc ccacgagagc ggagcgctct gtcctttttt ggcagtgaag
    13501 gaaagcttcg cgttgttgtt gaggaatttc gcatccacag gctgcatcgt caatgcgtcg
    13561 aaaagcatga gctgggcatc gcccgtcata tccttgtccg tgaggttcga cactttcgcg
    13621 gtgaacgtga tcttgtcacc ttcgcggaag aagcgcggtg cattcggcat gaccataaga
    13681 tctttctgcg tcaccacttc ttttgtcgtc tgcccgtaca taagatcttt cgtgtgcgcg
    13741 aacgccatga atttccactt cgtcagcgct tcgttcatcg tgaatttgat gatcaccgtg
    13801 ccggtggaat ccgtttccag ctgcgggaag aagaacaccg tttcattgag gttctgccgc
    13861 gccgcgatcg cattcaggtt gggtgcgttc cggtaatctt ttcccgccgc cgggctatcc
    13921 tgcatgaggg cggtggtttc cgtatttttt tctccggagg catgcgcggt gtttcccgat
    13981 gttgaaaatg cgaacgtcgg cgacccggtg gctgcatctg ccgggggcgg tgccgcaggc
    14041 gccttcgatt tatcgacggc tttcgactgg gcgatgtcca caactccgcc ggaaacacct
    14101 aaattatctt catccatttc ttcccttaaa taacccggtc cgttcccgtc atagccatac
    14161 cagtaattac cgtagctgaa ataaaacccg aaccagttga gctgatcata ggtgcgatac
    14221 tggtaatcgg gcaatgaatt atcattccag tacggcgcat aaagcgcaga tgtcgcagtt
    14281 ccttccaccc cgtgctgcca gtagagcgag ccgtaatacg atttgtagag gtaaaagctc
    14341 cagttgtggg aacggaactg gtcgagcgaa gcatcgtaca tcgcggccac catttccgcc
    14401 gccactttat cgcccttggg tcccttgatc tttattttcc attcttcttt ctgcccggga
    14461 ataagcttgt tgcggaacgt ttcgtattcg aaatcgagct gcttgctttc ccacggaacg
    14521 ctgacgccag catcactggt gtatgcctgg ttgtgataca ccatcgcaaa atgcacggca
    14581 aaatttccgc ggtgtttttc ttcaaccgga atttcaatgt atttctgctc ttcgttcagc
    14641 gtaagccact ggcggctggc aatttttccc tgtatctccg tttcgtaaag cactttcaca
    14701 ttcttaccgg atgtcccgac gaggaaaacc gctttgtcgc ccggctggca gttcgtgttg
    14761 atggcctgga acgaccacgc ttctttcgat gccggcattt tatccctgat atcgtacagc
    14821 gtgaaatatt ttacatcctt cacttcctgc ccgtatttgt cttttgccgt cgcctcgatg
    14881 atataggatc ccggcttcca ggaatctttg ttcttcagcc tgagcgattt ttccttgccg
    14941 gtattgtatt tcccggaata cacttcgctc cctttcgtcc agttcacctg cttgttctcc
    15001 tctttgtaag gaagatcagg gaacgtagtg gtccagtcgt ctttcgtata aatgaattta
    15061 tccggcacct gccactggcg gtccttgtaa attttcccgg gatcgttcag gcggtggatg
    15121 gaaacggata ccatcgacga ttcgggttcg ccggaaagat ttgtcagcga aacagggatt
    15181 cccgcggtgt cgctcttttc gatatagtta tccgcattca cgctcagctc cagcgccacg
    15241 taaccgacat tcacatacgc gctcgtgctg tgcgtttcgc ccgtgatgtc cgtcacgtcg
    15301 atgttgatcg tgtaattgaa tgtcggtgag ctggacttcg gaacggattt atccggcagc
    15361 gctttgaacg gaatgacgaa tccgcctgta tcgttcgtca ttacttctcc gttggaaata
    15421 acagcggtct ggctgttcgg gtaatatccc caccagtaat aataccacca cgggaagctg
    15481 gcggaacgcg tcaccgtgta cgtgactttt gctccgtcga cggccgaacc ggcgaaggtg
    15541 cgtgcgctgc ctttcacaaa aacgctgtcg ttcaaacggt atgcgccttt cacgggctgc
    15601 gttgtgatat cgaatttcgg gcgtttgtat tcttccacgc tgaaatactc gctgccggat
    15661 tcgttctgga tatgcatctg tcccgtgagc ccggcggaag gagcagtgaa ggttccgctg
    15721 aatgatccgt aatcgttcgt ttcgaggttc agcgaagtga ctttctggta attcacatcg
    15781 tagaacgtca ccgtcgtcgt cttgtgcggc aatatcttgt tgttgtcgcc gtcagtgttg
    15841 acgatgatcc ctttgaaata gatcgtctgc cccggccggt aaatgctgcg gtcggtgaag
    15901 aaaaaagtct gcggacgcat ctcgcgcggg gcattgtaag gtttgtactg gtatgatgag
    15961 ccggtgatga aatgattccc cccgctggaa agctcgagga agaacgtgcg gtaattgccg
    16021 gcgggcggaa cgttcacgcg gccatctttg tcggaaaaat agcggtcgcc ttttttgtaa
    16081 cggtaatcgc gcgtcacgta atcatacgac tggtaccaga cctgcgcgct gacaccttcg
    16141 agcggtccgc cgttctcgcg gtcgaagacc aggaaatcaa agctgccgtc ggtgagctgc
    16201 cgctgcacat acccgagcgt ggtgctccac atctcggtat aggcgatcgc atttttcatc
    16261 atcgtgaaat ccttgtccac ggaggaaagg atgacgtatt ttcctgcagg caggtcggga
    16321 atttttattt ccacgctgtg atcggcgtaa tcgctgtcgg cgggaagcga aaggtcccac
    16381 gactggagaa ccggcagcgc gagataatcc ttgatcacct gttcggtcgt ccggtaatcg
    16441 gttttcgcca cgtattcctg cggaacggaa gcgatgcgga aatagacatg actgaaattc
    16501 cggtacgtca gaagtccggg cgaaggcttc ccgggaacgg aagcatcctg cacggtgaag
    16561 gaaacgttct tggcggtgat gtcgttgcga atgatggaac actggtaggc gccgggcgtt
    16621 ccgggatatt tttgaatgac cgcattgcag atgtcgagcg cgcgcttgcg ttcccacctg
    16681 tatttttcat ctttccccgg gacgtactgc gcgccgagcg aattgtgata caccgccagc
    16741 tggtacagca cttcggcggt tattttgctg tcgggataat tcgcggcgag ctggcgcaac
    16801 cccatttcat acaggctgtc cttgtccggg tcgacagact tgttgtgcac gaaaagaagc
    16861 cgctccaatt ccacgtccac caacgcatcg ggatcttttt cggggaaatg cattttttca
    16921 agcgtctgca gcgtgcgcag cgcgtaaaaa tccatcgaca tcgtgtcggg ggaattcagc
    16981 ggaagcgtag tgaaaatgac ggccggagaa aagtaatcgg ggtta
//
LOCUS       DASTSF010000011         4428 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1551344,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000011 DASTSF010000000
VERSION     DASTSF010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4428
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1551344"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..771)
                     /locus_tag="VFU15_00270"
     CDS             complement(1..771)
                     /locus_tag="VFU15_00270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716231.1"
                     /translation="MKKTLALFFLLLITQRFFAAPPPSLPMAGGPKFGFSYAGSGVVS
                     FFSVDTRHSDKAVPRAGFGAMFRIHFYPSANAHIQIGLQVMSQSCAFNSYYFAPGYSV
                     LYDKTFGYTHTFRTLELWVPVMARIGLTPGEASARTIFYLLGGYSPKLYLGTTADIVQ
                     NATGKEVWGGTKDLQYEHQFLGANTGNELMGGFGFDKRFGFNEKFLSFETIFSYTLSR
                     FIYDLGRTGDNPLYIRNMSLQLQIGYRFAGGGHGGGVN"
     gene            complement(768..2450)
                     /locus_tag="VFU15_00275"
     CDS             complement(768..2450)
                     /locus_tag="VFU15_00275"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013749511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEU4716232.1"
                     /translation="MKIITRIFPLFFLLLFACKGKKQKADFILRNGKVYTADASFDVL
                     ESFAVKDGKIIAVGTNESIGAMFESDSILDAKGKTVLPGLIDAHCHFVGYSLGLQQCD
                     LVGTTSFDDVIKRVQDFAKTNKNEWIIGRGWDQNDWAVKEFPDRKMLDSLFPHTPVLL
                     KRVDGHAALVNGEALRRANITDSTGVEGGEIIREIAGVQKSGRHTTVIVYGAPTGILI
                     DNAVNLVEKVIPPANETQMRDALLQGQENCFADGLTTLDDAGLMKWQIDIIDQLQKKG
                     ELKMRIYAMLSDSLPNYEHYLSFGPYKTDYLDVRAFKFYADGALGSRGACLLKPYTDK
                     PGWQGFLLNSPAHFATRMKKALDAGFQVCTHCIGDSANRFILHLYDSLLPDSARRWRI
                     EHAQVVSKEDVPLFAHRLIIPSVQPTHATSDMYWAGKRLGDDRMKTAYAYNDLLSSAG
                     ILALGTDFPVENINPMYTFYAAVSRMDLQHFPQGGFQPENALSRRNTLLGMTAWAAWA
                     NFEEREKGSIGAGKFADFVILDNDPMTCAVEEIPLIHVLDTYVGGKKVFERK"
     gene            complement(2505..3269)
                     /locus_tag="VFU15_00280"
     CDS             complement(2505..3269)
                     /locus_tag="VFU15_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU4716233.1"
                     /translation="MSTRKTAFITGASAGFGKAIAELYAANGWNLVLCARRKVRLEVL
                     ERQLKEKHGVEIVSLAFDIRSNDEVKKAVASLSGKWKEIDLLVNNAGLASGLSTIQEG
                     SLDDWERMIDTNVKGLLYMTRCIAPLMTERKTGHIINIGSIAGKEVYANGNVYCATKH
                     AVDALTKAMRIDLLPFNIRVSQVAPGMAETEFSIVRFHGDSERAKNVYKGFEPLTAED
                     IAETVFWITSRPAHVNINDVVIMPTAQANSTTVIRK"
     gene            complement(3273..>4428)
                     /locus_tag="VFU15_00285"
     CDS             complement(3273..>4428)
                     /locus_tag="VFU15_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEU4716234.1"
                     /translation="NAPQIAGLRIIFDLPAVGINILITWLVLVGVKESKNVSNLMVII
                     KLVIIGLVIIVGAFYIDIDNWKPFLPNGFGGVMAGVSSVFFAFIGFDAVSTLAEECKN
                     PQRDLPRGMILSLLISTIIFVILALVLTGMAVYSSLNVPDPLADIFRMKGVKWMLYIV
                     SVSAVVAMTSVILVFQMGQPRIWMTMSRDGLLPKRFSSIHPKYKTPAFSTIVTGLVVG
                     IPIFFTDRNFILDFTSIGTLFAFTLVCGGVLVLPRRQRVTNDGKFYMPYVNAKYILAP
                     LVIVAFALILILKPSYFPDLLTVNEGNAVQSIPMIIFFVLCLVMSVLSFLKDLSLIPV
                     LGFLSCMYLFTGFSIPNWKWFGSWLVIGLVVYFLYGFRKSRLALANKTAS"
BASE COUNT          993 a         1209 c         1245 g          981 t
ORIGIN      
        1 ttaattcaca ccgccgccat gtccgccgcc tgcaaaccgg tagccgatct gcagttgcag
       61 gctcatgttg cggatgtaaa gcgggttgtc gccggttctt ccgaggtcgt aaatgaaacg
      121 ggaaagcgta tagctgaaaa tggtttcgaa cgaaaggaat ttttcattga agccgaaacg
      181 cttgtcgaaa ccgaagccgc ccatgagctc attgccggtg ttggcgccga ggaactggtg
      241 ctcgtattgc aggtctttcg ttccgcccca tacttctttt cccgtcgcat tctgcacgat
      301 gtcggccgtg gtgccgaggt aaagtttcgg ggaatagccg ccgagcaggt agaagatcgt
      361 gcgtgcggaa gcttctcccg gcgtcagccc gatgcgcgcc atgacgggaa cccacaattc
      421 aagcgtgcgg aacgtgtgcg tgtagccgaa cgttttgtcg tagagaacgg aatagcccgg
      481 cgcgaaataa taggagttga acgcacacga ctggctcatc acctgcaggc cgatctgtat
      541 gtgcgcattc gcagaaggat aaaaatgaat gcggaacatc gcgccaaaac ccgcacgcgg
      601 aacggctttg tcgctgtggc gtgtgtcgac gctgaaaaaa gagaccacgc ccgatccggc
      661 gtagctgaac ccgaatttcg gtccgccggc catcggcagg gaaggaggcg gtgccgcgaa
      721 aaatcgctgt gtgatcagca gcaggaagaa cagggccagt gttttcttca tttgcgttca
      781 aaaacttttt ttccgcctac gtaggtgtcg agcacatgaa tgagcggaat ttcttccact
      841 gcgcaggtca tgggatcgtt gtcaagaatg acaaagtcgg cgaacttgcc cgccccgatg
      901 ctgccttttt cacgctcctc gaaattcgcc caggcggccc aggcagtcat gccgagcaat
      961 gtgttccttc gcgaaagcgc attctccggc tggaaacctc cctgcggaaa atgctgcagg
     1021 tccatgcggg aaaccgcggc gtagaacgta tacatcgggt tgatgttctc cacgggaaaa
     1081 tcggttccca gtgcaaggat tcctgcagag gaaagaagat cattgtacgc gtaagctgtt
     1141 ttcatacggt cgtcgccgag gcgtttgccc gcccagtaca tatcgcttgt cgcatgtgtg
     1201 ggctggacgg aagggatgat caggcgatgc gcaaaaagcg ggacatcttc tttcgaaacg
     1261 acctgcgcat gctcgatgcg ccatcgcctc gcgctgtcgg gcaggagcga atcgtacaaa
     1321 tgaagaatga aacggttcgc ggaatcgcct atgcaatgcg tgcagacctg gaagccggcg
     1381 tcgagcgctt ttttcatgcg cgttgcgaaa tgtgcgggag aattcagcag gaatccctgc
     1441 cagccgggct tgtcggtgta gggcttcagc aggcacgcac cgcgggagcc gagcgcaccg
     1501 tcggcgtaaa atttgaatgc gcgcacgtcg aggtaatccg ttttgtacgg accgaaagaa
     1561 agataatgct cgtaattcgg aaggctgtcg gaaagcattg cgtagatccg catcttcagc
     1621 tctccttttt tctgcagctg gtcgatgatg tcgatctgcc atttcatcag tcccgcatca
     1681 tcaagcgtcg tcagcccgtc cgcaaaacaa ttttcctgtc cctgcaaaag cgcatcgcgc
     1741 atctgcgttt cattggccgg cggaatgacc ttctccacaa gattcaccgc gttgtcgatg
     1801 agaatgcccg ttggcgcgcc gtaaacaata accgtcgtat gccgccccga tttctgtact
     1861 ccggcgatct cgcggatgat ctctccgcct tcgaccccgg tggaatccgt gatgttcgcc
     1921 cgccgcagcg cttccccgtt cacgagtgcg gcgtgaccgt ccacgcgttt cagcagcact
     1981 ggtgtatgcg ggaaaagact gtcgagcatt ttccggtcgg gaaattcttt caccgcccag
     2041 tcgttctggt cccagccgcg gccgatgatc cattcgttct tattcgtctt tgcaaaatcc
     2101 tgcacgcgtt ttatcacgtc gtcgaacgat gtcgtcccga caagatcaca ttgctgcaga
     2161 ccgagcgaat aacccacgaa atgacaatgt gcgtctatca gtccgggtaa aaccgttttc
     2221 cctttcgcgt cgagaatgct gtcggattca aacatggcgc cgatgctttc attcgttccg
     2281 acagcgatga tttttccgtc tttcacggca aaactttcaa gcacatcgaa acttgcgtca
     2341 gcggtgtaaa cttttccgtt ccggagaatg aaatccgcct tctgcttttt tcccttgcag
     2401 gcgaaaagca aaagaaagaa gaggggaaaa atgcgggtga tgatcttcat gctgtcggtc
     2461 gctgtttcag ttcttccggt gcaccgggaa aagggcagcc cgttctattt ccggataacg
     2521 gttgtcgaat tggcctgggc cgtgggcatg atcacgacgt cgttgatgtt cacgtgcgca
     2581 ggacgggaag tgatccagaa aacggtttcc gcgatgtctt ccgcagtcag cggctcgaaa
     2641 cctttgtaca cgtttttcgc acgttcggaa tcgccgtgaa agcgcacgat ggaaaattcc
     2701 gtttccgcca tgccgggggc gacctgcgaa acacggatgt tgaacgggag aagatcgatg
     2761 cgcatggctt tggtcagcgc atccacggcg tgcttcgtgg cacagtatac gttcccgttc
     2821 gcgtaaactt cttttcccgc gatggagccg atgttgatga tgtggcccgt tttccgttcc
     2881 gtcatgagcg gcgcgatgca gcgcgtcatg tacagcagtc ctttcacgtt cgtgtcgatc
     2941 atgcgctccc agtcgtccag cgaaccttcc tgtatcgtgc tgagcccgga agcgagcccg
     3001 gcgttgttca cgagcaggtc gatctccttc cattttccgc tgagcgaagc gacggctttt
     3061 ttcacttcat cgttgctgcg gatatcgaac gcaagcgaaa cgatctccac gccgtgcttt
     3121 tctttcagct ggcgttccag cacttcgagc cgcactttcc gtctcgcgca gagaacgagg
     3181 ttccatccgt tcgccgcata aagttccgcg atcgcttttc cgaaaccggc actcgcaccg
     3241 gtgatgaaag ctgtttttct tgtggacata gatcaggatg ctgttttatt ggcaagcgca
     3301 agcctgcttt tccggaaacc gtaaaggaaa tagaccacga gcccgatcac gagccagctg
     3361 ccgaaccatt tccagttggg aatgctgaaa ccggtgaaca ggtacatgca cgaaaggaac
     3421 ccgagaacgg ggatgagcga aagatctttc aggaaggaaa gcacggacat caccaggcac
     3481 aggacgaaaa aaatgatcat cgggatgctc tgcaccgcgt tgccttcatt cacggtcagc
     3541 aggtcgggga aatacgacgg tttcaggatg aggatgagcg caaatgcgac gatcaccagc
     3601 ggtgcgagga tgtatttcgc attgacgtat ggcatgtaga atttcccgtc gttggtcacg
     3661 cgctgcctgc gcggcagcac gagcacgccc ccgcatacga gcgtgaacgc gaacagcgtg
     3721 ccgatgctgg tgaaatcgag aatgaagttc cggtcggtga aaaagatcgg gatgcccacc
     3781 acgagcccgg tgacaatcgt ggaaaaggcc ggcgttttgt atttcgggtg aatggacgaa
     3841 aaacgtttcg gcaggagccc gtcacggctc attgtcatcc agatgcgcgg ctgtcccatc
     3901 tggaacacga ggatcacgct cgtcatcgcc accacagccg aaaccgaaac gatgtacagc
     3961 atccacttca cgcccttcat ccggaaaatg tccgcgagcg gatcgggaac gttcagcgag
     4021 gaatagaccg ccatgcccgt gagcacgagc gcaaggatca cgaaaatgat cgtgctgatg
     4081 agcagcgaaa ggatcatgcc gcgcggaaga tcgcgctgcg gatttttaca ttcttccgcc
     4141 agcgtggaca cggcgtcgaa cccgatgaat gcgaagaaca cggacgacac gcccgccatt
     4201 acgccgccga aaccgttcgg gaggaaaggt ttccagttgt cgatgtcgat gtaaaatgcg
     4261 ccgacgatga tcaccagccc gatgatcacc agcttgatga tcaccatgag gttgctcacg
     4321 ttcttcgatt cttttacgcc caccagcacc agccacgtga taaggatgtt gatgccgaca
     4381 gccggcaggt cgaagatgat gcgcaggccc gcgatctgcg gtgcattc
//
LOCUS       DASTSF010000012        10213 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_878878,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000012 DASTSF010000000
VERSION     DASTSF010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 10213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10213
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_878878"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..372)
                     /locus_tag="VFU15_00290"
     CDS             complement(<1..372)
                     /locus_tag="VFU15_00290"
                     /inference="COORDINATES: protein motif:HMM:NF025092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEU4716235.1"
                     /translation="MKQVFPSGRILSLVICIFLTVLSYGQNVSGTVNNASGEPLEGAV
                     VMLNGNTTQTDNAGQFSIPCGTLPATINVHLIGYKDYSGTVSDCNAKLVIQMTAGETS
                     LQVVDINDSSSNTKNLAVANAS"
     gene            complement(476..970)
                     /locus_tag="VFU15_00295"
     CDS             complement(476..970)
                     /locus_tag="VFU15_00295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716236.1"
                     /translation="MQKFNAYLLVILFVAGSFLPAVMSVSLFLHRADVFDLMEKTGEQ
                     ESLLSLSPSEFAAIKWNNDHEFTFHNSHYDLFAVTKKAGNYILSCFSDKKEDEIINTL
                     DKTAAGERGAKKIAFRLMIFDHTDHGFAFRLLFSDFNPGMSITRSWNDAPRAITSPPP
                     RFMS"
     gene            complement(1105..1674)
                     /locus_tag="VFU15_00300"
     CDS             complement(1105..1674)
                     /locus_tag="VFU15_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002998852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEU4716237.1"
                     /translation="MRFVSDQAFDKISFKETPLEKAEYEGCVFRSCDLSECDLSGVRF
                     ADCEFIACNLSLAKVMRTAFRDVKFRDCKMLGLRFDTCNGIGLSFTFGNCELAHSTFY
                     KTKIKRTVFRDCRLNGCDFTECDVAESVFENCDLAGAAFENTNLEKADLRSAYNYSID
                     PETSRVKKAKFSQNGIAGLLDKYDIVIES"
     gene            complement(1671..1919)
                     /locus_tag="VFU15_00305"
     CDS             complement(1671..1919)
                     /locus_tag="VFU15_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012244327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716238.1"
                     /translation="MKKKTPATRTRAKNHVVYHKDGSVWAKGKMKDGQMHGYWEWFRK
                     DGVIMRSGYFENGKQAGEWTTYDREGNVYKVTQMKKAK"
     gene            2282..3340
                     /gene="rfbB"
                     /locus_tag="VFU15_00310"
     CDS             2282..3340
                     /gene="rfbB"
                     /locus_tag="VFU15_00310"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012508962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEU4716239.1"
                     /translation="MNVLITGGAGFIGSHVVRRFVTNYPEYHIYNLDALTYAGNLANL
                     KDVEAKPNYTFVKGDITDADFLQKLFSTHDFTYVVHLAAESHVDRSIANPIAFVVTNV
                     VGTVNLLNAAKHHWLNPGSGIRDQRFYHVSTDEVYGALGDEGFFTENTKYDPHSPYSA
                     SKASSDHFVRAYHDTYGLPVVISNCSNNYGSHHFPEKLIPLAINNIRNKKPIPVYGKG
                     ENVRDWLWVEDHARAIDVILHKGKNGETYNIGGHNEWKNIDLIRLLCRIMDKKLDRAE
                     GESEKLITFVKDRAGHDLRYAIDATKLKNELGWMPSLQFEEGLEKTVDWYLSNQEWLD
                     NVTSGNYQHYYEDQYEKR"
     gene            3340..4332
                     /locus_tag="VFU15_00315"
     CDS             3340..4332
                     /locus_tag="VFU15_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU4716240.1"
                     /translation="MHGKAWHTFDLSAKRFLVTGGAGFIGSHLVDYLVKHGAREVRVL
                     DDLSTGFIRNIEQHLDEKNFLLIRESITDESSCRKACAGIDFVLHHAALGSVPRSIDD
                     PVSTHNVNATGFLNMLVAAKEMNVKKFIYASSSSVYGDNEDEIKTEDRVGKALSPYAV
                     TKKLNEQYGKVFADVYGMDITGLRYFNIFGPRQSPTGAYAAAIPLFIDGLVNSKPVFI
                     NGDGEQSRDFTFVENVVQANMKACDAGNCAGEVFNIAAGGSISVKEVFALLKKITGSE
                     VNAQHLPERKGEIRNSRADISKAKNAFGYSPGVNVEEGLKRTVEWFTGSSPYNR"
     gene            complement(4304..5716)
                     /locus_tag="VFU15_00320"
     CDS             complement(4304..5716)
                     /locus_tag="VFU15_00320"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEU4716241.1"
                     /translation="MKANVLIFSAALVISTGIKADSICKRMSLFFDTDKSELSPVSAR
                     RLDSLVSIARKNESGYLLELYGHTDTVGNLTHNQTLSGSRMKSIEDYLDSKLQHTFTY
                     RENEMPETNTKVSGSMDANLAYNRRVDIFLIPMNGNNVVVPGKPNESVEIPIDHFGSC
                     GVCGSAPKVKSWYSADEAKGTNIQFQTNDSFKLMTAGTMLLDYQPCSSGGSGYGKDTS
                     TLVFRVCGQKPDKGITLWEADTMNGKIYWKPSDNKFSYDTATGCYVFRAPAGQLFNLD
                     KVYFDTLFRILLPASFAYNHAVLTRSSTVKVNDWKEDYDIAPNDTVCMLHALARDSGN
                     IYLLAAKVSLLPGSKVRTTTKLTTTFAPGETMYRQLTWSDTLVKIKRGKYAQKATVGF
                     YLPEYKEFLPADSVQGKYAFGKTPDCNYQYAYYTGKKLYVMSPKKAKVKTKKDVRDVR
                     FNRRSKKKFHRVSDYKAKSR"
     gene            5867..6094
                     /locus_tag="VFU15_00325"
     CDS             5867..6094
                     /locus_tag="VFU15_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018203666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1653 domain-containing protein"
                     /protein_id="HEU4716242.1"
                     /translation="MSIIPGKYRHYKGRLYEVLGTVRHSETLEELVLYKALYESEEFG
                     RDQLWVRPLPMLLEEVEVNGEHVPRFSFIAE"
     gene            6353..8635
                     /locus_tag="VFU15_00330"
     CDS             6353..8635
                     /locus_tag="VFU15_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007658447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HEU4716243.1"
                     /translation="MQSITEKLNIRKVAVELGITPQQVETTIGLLDEGGTVPFISRYR
                     KEMTGSLDEVQIMNVRDRMLVLREMEKRREFILSTIEEQGKMTDELRDKIVKADTITA
                     LEDIYLPYKPKRKTRATAAREKGLEPLALKLMEQTNFDVQEEAAKYVGAEKGVADADE
                     ALDGARDILAEMISENGDAREMLRKLFREKALVRSRVVAGKEEEGEKFRDYFNWDEAL
                     MQCPSHRMLAMRRGENEGILMLDIAPDADDAIDLLERKFLKAKNSAAEHVQAAIVGSY
                     KRLLQPSLETEFRQLTKEAADIKAIEVFAQNLRELLLAAPLGQKVILALDPGFRTGCK
                     VVVLDKQGKLLEHDVIFPHHGVREKREAEDTLFRLLAKHHVEAIAIGNGTASRETEEF
                     VRRIDELPKEIAVVVVNESGASIYSASEVAREEFPDKDVTVRGAVSIGRRLADPLAEL
                     VKIDPKSIGVGQYQHDVDQPLLKKKLDEVVESCVNAVGVELNTASRQLLSYVAGIGPS
                     IAKSIVEFRDEHGPFRSRKDLLDVPRLGEKAFEQAAGFLRIRNGKNPLDRTAVHPESY
                     HIVEAMAKDLGCGPEELIAEKELRKKIDVKKYVTEKTGLPTLNDIIAELDKPGRDPRK
                     TFEVFSFQEGISRIEDLREGMRIPGIVTNVTNFGAFVDVGVHQDGLVHISQLSDQFVD
                     DPSKVVKAGQKVMVTVTEVDVARKRIALSMKGEKSHPGKTQEKKISVPKNNGGTSSKK
                     SPGGNDWQDQLAALKGKFGS"
     gene            8648..9262
                     /locus_tag="VFU15_00335"
     CDS             8648..9262
                     /locus_tag="VFU15_00335"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008272131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEU4716244.1"
                     /translation="MNFDFTKDILPENERVRLRPIEISDWDNLLPVALADNDLLHYSP
                     SQVHSEGLLKKYIATALSDREKEVRYAFSVFDKSAGKYAGSTSFGNIVNKDKRIEIGW
                     TWIGRQFQRTGLNRNMKFLMMQYVFETLEFERLEFRTDERNAASRTAIEKIGGKYEGT
                     LRSHMLMPDGFRRNTACYSILKEEWPALKKQLIRTDTSHSLGIL"
     gene            9514..>10213
                     /locus_tag="VFU15_00340"
     CDS             9514..>10213
                     /locus_tag="VFU15_00340"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017730235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsB/CapC family capsule biosynthesis tyrosine
                     phosphatase"
                     /protein_id="HEU4716245.1"
                     /translation="MSLFNRIFHKTLPGEPVDLGVLRCDIHSHFIPGIDDGAKTMKDS
                     LELLRGMEELGYKKVITTPHIMSDFFRNTPEIISTGLEQLRNAAANEGIGIDIEAAAE
                     YYLDFELEKKLAEKRLLTFGEDYLLFEISYMNAPDNLDGFIFTLQTEGYKPVLAHPER
                     YPFWYTRSLENFEKLKDKGVLFQLNINSLTGHYSPATKKAAEQMIDNGWYEFIGSDCH
                     HMGHIDLMKKARNEN"
BASE COUNT         2590 a         2736 c         2586 g         2301 t
ORIGIN      
        1 cgacgcgttt gcaaccgcca ggttttttgt atttgaagaa ctgtcattga tgtcaacgac
       61 ctgcaggctg gtttctcccg ctgtcatctg tatcaccagt tttgcattgc agtcggaaac
      121 cgtaccactg taatccttat aaccgatgag gtgaacatta atggtggccg gaagtgttcc
      181 gcacgggatg gaaaactgcc cggcattatc tgtttgtgtg gtatttccgt tgagcattac
      241 aacggcgcct tcaagtggtt cgcctgaagc attgtttact gttccactca cattctgtcc
      301 gtatgaaaga acagtaagga atatgcatat aacaagggag agtattctcc cggatggaaa
      361 aacctgtttc atgattttga agtgtggtta aataagatac tccaggggat tcttcccccg
      421 gtaaaagaca cctgtatata agcacagaaa aaatctgcgt atatacacaa cacaatcaac
      481 tcatgaaacg cggtggagga gaagtaatgg cacgcggagc atcattccag gaacgggtaa
      541 tgctcatgcc cggattgaaa tctgaaaaaa gaagtcggaa agcaaaacca tgatcagtat
      601 gatcaaaaat catcaaccgg aaagctattt ttttcgcacc ccgttctccc gccgcggttt
      661 tgtcaagtgt attgataatc tcgtcttctt ttttatcact gaaacaggac agaatatagt
      721 tcccggcctt ttttgtcaca gcaaaaagat catagtggct attatggaaa gtgaattcat
      781 gatcgttatt ccatttgata gcagcaaatt ctgaaggaga aagtgaaagc agcgactcct
      841 gctcacctgt tttttccatc aaatcaaaaa catctgcacg gtgcagaaaa agagaaacag
      901 acatcaccgc aggcagaaaa cttccggcaa cgaacaggat gaccaacagg tatgcattga
      961 acttctgcaa caaatgcaaa tataaaaaaa gacgggtgct tgcctactgt accggctgac
     1021 aagtcgttct tcccctcaca gtatacagcc gcaaactttt cccgggaaac cttccctgcg
     1081 gttttaaaag acaaccgcac ccgctcagct ctcgatcacg atatcgtatt tgtccagcag
     1141 cccggcgatc ccgttctgcg agaacttcgc cttcttcacg cggctggttt ccggatcaat
     1201 ggaatagttg tacgcgctgc gcaaatccgc tttttcaagg ttcgtatttt caaatgcggc
     1261 tcctgcaaga tcacaatttt caaaaaccga ttccgccacg tcgcattcgg tgaaatcaca
     1321 tccgttcagg cggcagtcgc ggaacacggt ccttttgatc ttcgttttgt aaaaagtgga
     1381 atgcgccagc tcgcaattcc cgaaagtgaa cgaaagaccg atgccgttgc aggtgtcgaa
     1441 acgcagaccg agcatcttgc agtcgcggaa tttcacgtcg cggaacgcag tcctcatcac
     1501 cttcgcaaga ctcaggttgc aggcgatgaa ttcacaatcc gcgaagcgca caccggagag
     1561 atcgcattcg gaaagatcac agctgcggaa aacgcagcct tcgtattcgg ctttttcaag
     1621 gggcgtttct ttgaacgaaa ttttgtcgaa cgcctggtcg gaaacgaacc tcattttgct
     1681 tttttcattt gcgttacctt gtatacgttg ccttcccggt cgtacgtcgt ccattcaccg
     1741 gcctgtttcc cgttttcgaa ataaccggaa cgcatgatca cgccgtcttt ccggaaccat
     1801 tcccagtagc cgtgcatctg cccgtctttc atttttcctt tggcccagac cgaaccgtcc
     1861 ttgtggtaaa cgacatggtt tttcgccctt gttcttgttg ccggagtttt ctttttcatg
     1921 ggactatttt cgcgtgcgat tcagtcgcgt tttgcaattt ccgttcgaat gatccggcaa
     1981 tgcccggacg ttttttcata aaagtccgcg cccgtaatgc cgccgcaggt aaaaaaccgc
     2041 tgtgcgaaag cttcacgacc ggcagtaacc ttcattggcg gatccgtttg aaattcaaac
     2101 ttacacaacc cgtctcccga ttccaaccgg gatcaccaaa ccttaagaaa cttttaaacc
     2161 tgccagcttt ccaggctttt caacttccca acttttcaat tttccaactt tgaatagaat
     2221 tattcctgcc cgaaaccccg tattttagct ttccatttca aaccaccagc gcttgagcaa
     2281 gatcaacgtc ctcatcaccg gcggagccgg cttcatcggt tcgcacgtcg tgcgccgttt
     2341 cgttacgaat tatccggagt accatattta caacctcgac gcgcttacat acgcgggcaa
     2401 cctcgccaac ctgaaagacg tcgaagcgaa accgaattac acgttcgtga aaggcgacat
     2461 caccgacgcg gatttcctgc agaagctgtt cagcacacac gatttcacct atgtcgttca
     2521 ccttgccgcc gaatcgcacg tcgaccgctc gatcgcgaac cccattgcgt tcgtcgtgac
     2581 caacgtcgtg ggaacggtga atttgctgaa cgcagcgaaa catcactggc tgaacccggg
     2641 atcgggaatc cgggaccaga ggttctacca cgtctctaca gacgaagtgt acggcgcgct
     2701 cggcgatgaa ggtttcttca ccgagaacac gaaatacgat ccgcattccc cctactctgc
     2761 ttcgaaagcg agctccgatc attttgtgcg cgcgtaccac gacacgtacg gattgccggt
     2821 tgtcatttcc aattgctcca acaattacgg ctcgcatcat ttcccggaaa aattgattcc
     2881 gcttgcgatc aacaatatcc gcaacaaaaa acccatcccg gtttacggga aaggagagaa
     2941 cgtgcgcgac tggctgtggg tggaagatca tgcacgcgcg atcgacgtga tcctgcacaa
     3001 agggaaaaac ggcgaaacat acaacatcgg cgggcataac gaatggaaga acatcgacct
     3061 catccgcctg ctgtgccgaa tcatggataa aaagctggac cgcgcggaag gtgaatccga
     3121 aaagctgatc acgttcgtga aagaccgcgc cggccacgac ctgcgctacg cgatcgatgc
     3181 gacgaaattg aaaaatgaac tgggctggat gccttcgctg cagttcgagg aaggtcttga
     3241 aaaaacagtc gactggtacc tctccaacca ggaatggctc gacaacgtca cgtcgggaaa
     3301 ttaccagcat tactacgaag accaatacga aaaacgctga tgcacggaaa ggcgtggcat
     3361 acgttcgatc tttccgcgaa acgttttctc gtcacgggag gagcgggctt catcggctcg
     3421 catctcgtgg attacctcgt gaagcacgga gccagggaag tgcgcgtgct cgatgacctc
     3481 tcgaccggtt tcatccggaa catcgaacaa cacctggatg aaaaaaattt cctgctcatc
     3541 cgcgaaagca tcacggatga atcttcctgc aggaaagcct gcgcgggcat cgatttcgtg
     3601 ctgcatcacg ctgcgctcgg ttccgtcccg cgttccatcg acgatccggt ttctacgcat
     3661 aacgtgaatg cgacaggatt cctgaatatg ctcgtcgctg cgaaagaaat gaacgtgaag
     3721 aaattcattt atgcgagttc gtcttctgtt tatggcgata atgaagacga gatcaaaacg
     3781 gaagatcgcg tgggaaaagc attgtcgcct tatgcagtga ccaaaaagct gaacgaacaa
     3841 tacgggaaag ttttcgcgga cgtatacggc atggacatca ccggcctgcg ctacttcaat
     3901 attttcggtc cccggcaaag tcccaccggc gcgtatgcag cggccattcc tctttttatc
     3961 gacgggctgg tgaacagcaa gcctgttttc atcaacggcg acggggagca gtcgcgcgat
     4021 ttcaccttcg tggagaacgt cgtgcaggcg aatatgaaag cgtgtgatgc ggggaactgt
     4081 gcgggcgaag tcttcaatat cgctgcgggc ggaagcattt cggtgaaaga ggtttttgca
     4141 ttattgaaaa agatcaccgg gtcggaagtg aatgcgcaac atcttcctga aagaaaaggc
     4201 gagatccgga attcccgcgc cgacatttcc aaagcgaaaa acgcgttcgg ctattcaccg
     4261 ggagtgaacg tggaagaagg attgaagcgg acggtggaat ggttcaccgg ctcttcgcct
     4321 tataatcgct gacgcggtga aactttttct tcgacctccg gttgaaccgc acatccctta
     4381 catctttttt cgttttcacc ttcgcttttt tcgggctcat cacgtacagc tttttgccgg
     4441 tgtaatacgc atactggtaa ttgcaatccg gtgttttccc gaaggcatat tttccctgta
     4501 cactgtcggc cgggagaaac tccttgtatt cgggcaggta aaaaccgacc gtcgctttct
     4561 gcgcgtactt tccccgtttt attttcacca gcgtgtccga ccatgtgagc tgccggtaca
     4621 tggtttcgcc cggcgcaaac gtcgtggtca atttcgtcgt ggtgcgcact ttcgaaccgg
     4681 gcagtaggct cactttcgcc gccagcaggt aaatatttcc gctgtctctc gccagcgcat
     4741 gcagcatgca caccgtatca ttcggcgcga tgtcgtaatc ctccttccag tcgttcactt
     4801 tcacagtgga tgagcgcgtc agcacggcat ggttgtaagc gaacgaagcg ggcagcagga
     4861 tccggaacag tgtatcaaag taaaccttgt cgagattgaa aagctgcccg gccggcgcac
     4921 ggaaaacata acaaccggtc gcggtatcgt agctgaattt attgtccgac ggcttccagt
     4981 aaattttccc gttcatcgtg tcggcttccc agagcgtgat gcccttgtcc ggtttttgtc
     5041 cgcacacgcg gaacacgagc gtcgaagtat cttttccgta cccggatcct ccggaggagc
     5101 agggctggta atccagcagc atcgtgccgg ccgtcatcag cttgaaagaa tcattcgtct
     5161 ggaactgtat gttcgtgcct ttcgcttcat cggcgctgta ccaggatttc actttcggcg
     5221 ctgagccgca aacaccgcag ctgccgaaat gatcgatcgg gatctcgacc gattcgttgg
     5281 gcttcccggg aacaaccaca ttgttgccgt tcatcgggat gagaaaaata tccacgcgcc
     5341 ggttgtatgc gaggttcgca tccatcgacc cggaaacttt cgtattggtt tccggcatct
     5401 cgttctcccg gtaggtgaat gtgtgctgta gttttgaatc caggtagtcc tcgatcgatt
     5461 tcatccggct ccccgagagc gtttgattat gcgtgaggtt tccaacggtg tccgtgtgtc
     5521 cgtataattc cagcaggtaa ccggactcgt ttttccgggc aatggaaacc aggctgtcca
     5581 gcctgcgcgc tgaaacgggc gacagttccg atttatcggt atcgaaaaaa agcgacatgc
     5641 gcttacagat ggaatccgct ttgatcccgg tggagatgac aagtgcggca gaaaagatca
     5701 ggacgttggc tttcatgtcc gtataaccgc acggtacggg aaggttacag cgaagaaaaa
     5761 caggaacgca caaagtttaa agtaaccaaa aacgcctccc ggaaattatt attgctggtt
     5821 gcgctgcatt accgccgggt gttccaaaac gcatattttt aatgcgatgt caatcattcc
     5881 cgggaaatac cgtcattaca aaggcaggct gtacgaagtt ctcggcaccg tgcggcattc
     5941 cgaaacactg gaagagctgg tgctgtacaa agcgctctat gaatcggaag aattcggcag
     6001 ggaccagctg tgggtgaggc cgctgccgat gttactcgaa gaagtggaag tgaacggaga
     6061 acacgttccg cgtttttcat tcatcgcgga ataagcgaac acctcagcgt ttttttactc
     6121 attgatgacg agatcagccg tcgtggctgc gtctgaattt ctgctgtgaa acttcgtggc
     6181 ccggttttcc tgcgacggga attcattccg gaaatgagtc gctgcagatc cgcgctttcc
     6241 tgcattggca aattactttt ccacacccgt tgaaaagttc tccgcgtctc cgcgtccctg
     6301 cggtcatttt tcagcatttc cgctgctatt tttgctttcc ataattcagc aaatgcaatc
     6361 catcaccgaa aaactcaaca tccgtaaagt cgccgtcgag ctcggcatca ctccgcagca
     6421 ggtagaaacc accatcggtc ttctcgacga aggcggaacg gttcccttca tttcccgtta
     6481 ccggaaagaa atgaccggct cgctcgatga agtccagatc atgaacgtgc gcgaccgcat
     6541 gctcgtcctg cgcgagatgg aaaaacgccg cgaattcatc ctttctacga tcgaagagca
     6601 gggaaaaatg acggacgagc tgcgtgacaa gatcgtgaag gcggacacga tcaccgcgct
     6661 ggaggatatt tacctgccgt acaaaccgaa gcgcaagaca cgcgccacgg ccgcccgcga
     6721 aaaaggtctc gaaccgctcg cgctgaagct gatggaacaa acgaatttcg acgtgcagga
     6781 agaagccgcg aaatacgtcg gcgcggaaaa aggtgtggcg gatgccgatg aggcgcttga
     6841 cggtgcccgc gacattctcg ccgagatgat cagcgagaac ggcgatgcac gcgaaatgct
     6901 ccgcaaatta ttccgtgaaa aagcgctcgt gcgcagccgc gtcgttgcag gaaaagaaga
     6961 agaaggcgaa aagttccgcg actatttcaa ctgggacgag gcgctcatgc aatgcccttc
     7021 acaccgcatg ctcgccatgc gccgcggcga aaatgaagga atactcatgc tcgacatcgc
     7081 cccggatgcg gacgatgcga tagacctgct cgaacgtaaa ttcctcaaag cgaagaacag
     7141 cgctgccgaa catgtgcagg ccgccatcgt gggttcatac aagcgactgc tgcagccttc
     7201 gctcgaaacc gaattccgcc agctgacaaa agaagccgca gacataaaag ccatcgaagt
     7261 ttttgcgcag aacctgcgcg agcttctcct cgccgccccg ctcggacaga aagtgatcct
     7321 cgcgctcgat cccgggttcc gcacgggatg taaagtggtc gttctcgaca aacagggcaa
     7381 gctgctcgag cacgacgtta tcttcccgca tcacggtgtg cgtgaaaaac gcgaagcgga
     7441 agacacactt ttccgtcttc ttgcaaaaca tcacgtcgag gcgatcgcca ttggcaacgg
     7501 cacggcaagc cgcgaaacgg aagaatttgt ccgccgcatc gacgagctgc cgaaagaaat
     7561 tgcagtcgtc gttgtgaatg agagcggcgc gtccatttat tctgcgtcgg aagtggcgcg
     7621 cgaagaattt cccgacaagg acgtcaccgt gcgcggcgct gtttccatcg ggcgcaggct
     7681 cgccgatccg ctggcggaac tcgtgaagat cgacccgaaa tccatcggcg tgggacaata
     7741 ccagcacgat gtcgatcagc ctttgctgaa aaagaagctc gacgaagtgg tggaaagctg
     7801 cgtgaatgct gtcggcgtgg aattgaacac cgcgagcagg cagctgcttt cctatgtcgc
     7861 cggcatcggg ccttccatcg cgaaaagcat cgtggaattc cgtgacgagc acggaccgtt
     7921 ccgttcacgc aaagacctgc tcgatgtacc gcggctcggc gaaaaagcat tcgagcaggc
     7981 agcgggattt ctccgcatac gcaacgggaa aaacccgctc gaccgcacgg ccgttcaccc
     8041 ggaatcgtat cacatcgtgg aagcgatggc gaaagatctc ggctgcggcc cggaagagct
     8101 catcgccgaa aaagaattgc gcaaaaagat cgacgtaaaa aaatacgtca cggaaaaaac
     8161 cggcctgccc acgctgaacg acatcatcgc ggaactcgac aagcccggcc gcgatccgcg
     8221 caaaacattc gaagtgttca gcttccagga aggcatcagc cgcatcgaag acctgcgcga
     8281 aggcatgcgc attcccggca tcgtgacgaa cgtgacgaac ttcggcgctt tcgtcgacgt
     8341 gggcgttcac caggacgggc tcgtgcacat cagccagctc tcggatcaat tcgttgacga
     8401 tccgtccaaa gtggtgaaag cgggacagaa agtgatggtg acggtgacgg aagtggacgt
     8461 ggcgcgcaaa cgcatcgcgc tttccatgaa aggagaaaag tcccatccgg gaaaaacgca
     8521 ggaaaagaaa atttctgtcc cgaaaaataa cggcggtacc tcttccaaaa aatcaccggg
     8581 cggcaacgac tggcaggacc agctcgctgc gctgaaagga aaattcggca gctgatcatt
     8641 catttctatg aacttcgatt tcacaaaaga cattctcccc gagaatgaac gcgtacgtct
     8701 tcgcccgatc gaaatatccg actgggacaa ccttcttccg gttgcactcg ccgataacga
     8761 cctgctgcac tattcgcctt cgcaggtcca ttccgaaggg ttgctgaaaa aatacattgc
     8821 gactgcctta tcggacaggg aaaaagaagt ccgctatgca ttttctgttt ttgacaaatc
     8881 agcgggaaaa tatgcgggca gcaccagctt cggcaatatc gtgaacaagg acaaacgcat
     8941 cgagatcggc tggacctgga tcggcaggca attccagcgc acgggactga acaggaatat
     9001 gaaattcctg atgatgcaat atgtttttga aacactggaa ttcgaacggc ttgaattccg
     9061 caccgacgaa cgcaatgcgg cttcccggac tgcgatcgaa aagatcggcg ggaaatacga
     9121 aggaacgctc cgcagccaca tgctcatgcc cgatggtttc cggagaaaca cagcatgcta
     9181 cagcatcctg aaagaggaat ggcctgcatt gaaaaaacag ctgatccgta cggataccag
     9241 tcattcgctg gggattttgt aatacaatgg gattgcttac caatcggggc cgctggtttt
     9301 gtcaaatccg gtcccggcga cagtatatgc ccggaatacc gcgcggtata cgacagcatg
     9361 aacaagctct ttctccaggt gccgcaggtc gaccttgtaa gcaatacagt cacgatcgaa
     9421 gtacggtgat cgccggtcgc gcgttgttct tttcatagtg ttttcgcaac atttcgtccg
     9481 ccaactaatc cctatcttcg gattcagttt cccatgagcc tcttcaatcg catcttccac
     9541 aaaacccttc ccggcgaacc cgtcgacctc ggcgttctcc gctgcgacat ccattcgcat
     9601 ttcattcccg ggatcgacga cggtgcgaaa acgatgaaag attcgctgga gctgctgcgc
     9661 ggcatggaag agctcggcta caaaaaagtg atcaccacgc cgcacatcat gagcgatttt
     9721 ttccgcaata cgcccgagat catcagcacg ggactcgaac agttgcggaa cgccgcggca
     9781 aacgaaggca tcggtattga tatcgaagcg gcggcggaat attacctcga cttcgagctt
     9841 gaaaaaaaac tggcggaaaa acgcctgctc accttcggtg aagactacct gctgttcgag
     9901 atctcgtaca tgaatgcgcc ggataatctc gatggattta ttttcaccct gcagaccgaa
     9961 ggctacaaac ccgtgctcgc ccaccccgaa cgttacccgt tctggtacac caggtcgctg
    10021 gagaattttg aaaagctgaa agacaaaggc gtgctgttcc agctgaacat caattcactc
    10081 accggccatt attcacccgc cacgaaaaaa gcggcggagc agatgatcga caacggctgg
    10141 tacgaattta tcggaagcga ttgccaccac atgggccaca tcgacctgat gaaaaaagcg
    10201 cgcaatgaga att
//
LOCUS       DASTSF010000013        13706 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1997052,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000013 DASTSF010000000
VERSION     DASTSF010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 13706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13706
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1997052"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2005
                     /locus_tag="VFU15_00345"
     CDS             <1..2005
                     /locus_tag="VFU15_00345"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEU4716246.1"
                     /translation="NAGYTGNWWGVVADASGQNPIQQGPTDPYPGMYVSTTSLVNSNY
                     AIPNPLRYVNSETVPFIAIPTNVLASGNIIVGDVAYVYNTTTGQSCFAIYADAGNTTS
                     IGEASIMTASLVGVDPDVRTGGTSQGIIDYVVFPHSGFGQGYIPTIAQIDSIGNLMLA
                     TVGGPCIASCIGPVYNNQPPSTAVNTPAGWDTTSFTATFSDINNGCSAGIEKTFYQVS
                     DYNTNNEWRANNANGFFNDDFNGSAISPGWTSVAGTWAISGNALYESDENSSNTNIYA
                     PLAQNLSDCYLYEWTGTMDGSGNNRRAGFHFFCDQPDSSNRGNSYFVWFRLDDDKIQI
                     YKVINNSWGNNPVLDTAYNFTANQSYDFKVMYDRISGVIRVYVNDVSCAQWVDSLPIA
                     NGSYVSFRNGNCNYKVDNFKVFRSRSSSALVSAGTGNGNDIRFENHDPATPAGRISSV
                     VTDRMRGISAVSDQYVNTDFTHPSSPLAVNDGTASDIDTTFNGTQLEANWSACADPNS
                     HIRAYYFAIGTTPGASDVIPWTSNGINTSVTVTGLTLMHQQQYYFSVLSEDSAMMQSA
                     VTVSDGQLYFDVTTEAGLHSADNSQPPLLYPNPSPGVFTIGGTFSGDFSMTVFSQDGK
                     MVMNETREENERNVDISSQPDGIYFVRITAGGKTHLQKVVKAE"
     gene            complement(2125..5178)
                     /locus_tag="VFU15_00350"
     CDS             complement(2125..5178)
                     /locus_tag="VFU15_00350"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HEU4716247.1"
                     /translation="MRRKKLLKELRAILIFLPALIIPLVMAFYPGGEQASASFVFPKT
                     VFPSRSLLPDTLPPPFLENETDRWVDSVMKTMTQDEKIGQLFMVAAWSNKDAKHTKEI
                     TDLIAKNHIGGLIFMQGGPVRQAQLTNKYQKLSKIPLMIAMDAEWGLSMRLDSTVQFP
                     REMTMGAMDDDSLVYKMGIEMARECKRLGVNVSFSPVADVNCNPANPVIGTRSFGENK
                     FMVAHKAVMYMKGLQDGGVMACGKHFPGHGDTDSDSHKTLPVINHDKARMDSVELFPF
                     KQLIRNGLGSMMVAHLYIPAYDTTTNLATTLSKNVVTGLLKEQLGFRGLVFTDALNMK
                     GVSQFYKPGEVDLKALLAGNDVLLFSGDVPKAVAMIRGSIAKGELTQADVDAHCRKIL
                     AAKKWLGLDKKKTVKQKNLWEDLNTPGAAWLCRQMAEKAVTLLKNDNTLIPFTHLDTM
                     KIASLAVGRNDSVVFQQRLGMYAPVTDFSLMRDCKTAERDTVLKKLQAFNCVVLSLHG
                     VSQKPAAGFGIGDLDVLLIDTLLKMKKHVVLDVFGPAYSLNFLPRAESCDAVILSYEN
                     QDYLEDISAQIIFGGVSASGKIPVTTNDWKAGSGLTTPAPTRFKYTMPEEVGFSPGAL
                     AKIDDIVKKGMDAKAFPGCQVFAAKDGKVFLYKSYGRFTYDPNSHLVRNDDLYDIASV
                     TKIMATVPAVMLMKDKGEIDLDKTLGDYLPELKGTNKEHIIIREMLAHQAGLQAWIPF
                     WMKTEDKDGNYDKSIWRKSWSDTFSIYVAPGLFMNKNYIDTIYKRINESPVKDDHKFV
                     YSDLGYYYLKKIIERKSHMLLSDYANRNFYFPLGLTTIGYQPRLRFPSSRCAPTENDK
                     DFRKQQLQGDVHDQGAAMLGGIGGHAGVFSDANDVGVMMQLYLNYGTYGGRRYFDSAT
                     VAQFTSVAYPGSGNRRGIGFDKPEPDVKKGNPACDSISLRSFGHQGFTGTQAWADPET
                     GVVFVFLSNRVYPDAKPNRLAEMGIRGDIMQVIIEEFREKKVNNR"
     gene            5549..7396
                     /gene="mutL"
                     /locus_tag="VFU15_00355"
     CDS             5549..7396
                     /gene="mutL"
                     /locus_tag="VFU15_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013664765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEU4716248.1"
                     /translation="MQDVIRLLPDSVANQIAAGEVIQRPASAVKELLENSVDAGATSI
                     KLIVRDAGRTLIQVVDDGKGMSETDARLSFERHATSKIRHADDLFNLHTMGFRGEALA
                     SIAAIAHVELKTRREEDEVGTAIVIEGSVFRSQEPVAVPPGTSFSVKNLFFNVPARRN
                     FLKSDAVEFRHIVEEFERVAFAHPQIAFSLTHNGNELFNLQKGNFRQRIISLYGNPYN
                     PRLVPVEETTDIVSVSGFIIKPEFSKKTRGEQFFFLNKRFIKNPYLHHAVQAAYEQLL
                     PADTYPSYFLFLDVDPKTIDVNIHPTKTEVKFEDERAVYAIIRSTVKKSLGQYNIAPS
                     LDFEQEMSMEIPYRRPGDPVSAPGITVDTSYNPFSDSKNGAGGKGHTHVTHFSPTEKS
                     NLSNWEELFTAHMNAGVIRSETEKEETTAERKKEEFSAKIWQLHERYLLSPIRSGILV
                     IDQQRAHERVLYERYLKIFSGQKMNSQQLLFPETAEFSPSNTALVSGLLDEINQLGFD
                     LRPFGSNTFVIHGVPPGSEEKNALQMLEGMLDNFRQNEQELHLERAENLARSLAKQAA
                     VKPGRAMTAEEMQALVDELFACETPSHAPDGKPAFINFPVEEIDKRFRR"
     gene            7439..8107
                     /locus_tag="VFU15_00360"
     CDS             7439..8107
                     /locus_tag="VFU15_00360"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEU4716249.1"
                     /translation="MRHSQFPPGVLNLIIINAIFYFAKMLVLSSRGVDLDGYLGLFHP
                     FSPAFHWYQYITSLFMHASVTHLVMNMFGLWLFGHMLENAYGTKRFLIFYFVSGVGAS
                     MIFQLWSSILHYQQLSGFGSFTDVMRTYPPGDLLIGASGCVYGVLMGAALLFPNTQMS
                     FLFLPGLPMKLKWVAVLYGGAEVYNAWQAAPGDNVAHFAHVGGMIFGFILVKIYSRDR
                     THFY"
     gene            8110..8970
                     /locus_tag="VFU15_00365"
     CDS             8110..8970
                     /locus_tag="VFU15_00365"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEU4716250.1"
                     /translation="MSIVADIRNSFRGTYAGLMRLIVINLGVFIVLNVIVEIMRISMT
                     PDAEELVAQYFVLPSSLPDLFPHIWTLITYMFTHFGFGHLFFNMLWLFFLGKLFCEQL
                     GPKRIIGTYVFGGVIGGLLFVLVCNLVPAFRYSSLEGASAGVMAVVVTIGTYAPNLRM
                     TIFPFRFEVQLKWIVLVAFLLTSVIYLSENTGGKVAHIGGALFGFIYGWQLKRGRTFF
                     DGITQLLSFKRSKLRVEHRRSISDEQYNLQKMNVKKRIDEILDKISRSGYDSLNREEK
                     DFLRKYHDKL"
     gene            8992..10131
                     /locus_tag="VFU15_00370"
     CDS             8992..10131
                     /locus_tag="VFU15_00370"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEU4716251.1"
                     /translation="MKNDNGETESKTAPPARKKGRIANAVNWTFILLLGLSCLAPFVN
                     PAVFWPLAFSGLLFPLFFLVNALLLLWWTIRRKRRGFYSLAMFLLSLPMYFRQAAVFG
                     TKEKSPPGAVKIMSYNVKLFDLYNWTGNRESRAEIFSLLKAQQPQLLCLQEFFNQDTG
                     RFRNLDSLKKLLALPYAHTEYTISLHGDDHWGMATFSQFPIVNEGKIVFNNRSNNICI
                     YTDILMNGDTVRVYNMHLQSISFGYADLKFLREMSKAEQSADELENSRNILRRMKRAY
                     IRRANQSQAVADHIAQCPYPVIVCGDFNDTPTSYTYRTIGKNLEDSFLESGRGFGKTF
                     INPFPFPRIDYIFHSSQFSTWQFNVIRTDNLSDHYPVECMIRKNK"
     gene            complement(10132..11097)
                     /locus_tag="VFU15_00375"
     CDS             complement(10132..11097)
                     /locus_tag="VFU15_00375"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HEU4716252.1"
                     /translation="MSFLKKYWATLRRMRILHVLHNMTHFGKLKKNKTLYRKYGISKS
                     VVGSISHAHISKPGAEIPWLDRPDAEKMLAGNPGLEKFPLQWQEELQKWPARGFMILE
                     GFASGETCDRINSELDELIREKKVDFDYTNSRVMNAWEHSPAIRAITKDRLLKEFLSF
                     TLGRETVPFQTISFLKGSRQKTHSDFIHMTTEPVGYLAATWMALEDISASGGPLHYFP
                     GSHRLPYILGEDFDHSSNALVIGDDLYGNYEKKIAELVSEKKLQKEIFLAKKGDLLVW
                     HANLLHGGEPVTDENASRKSLVTHYFCKGDIICYHEITQRPAILP"
     gene            complement(11178..12338)
                     /locus_tag="VFU15_00380"
     CDS             complement(11178..12338)
                     /locus_tag="VFU15_00380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716253.1"
                     /translation="MKKAFIFLLPFVLMFSCRKDDEVYIPENFVRYYGTSGDEMARKV
                     LRLPGGDLMIAGYGLGPNGGNDVILLKTDMHGNQKWMKYYGGAGNEQCWSVDLCDDGN
                     VVLGATTSSQGAGADDFWIIKADTAGNVIWSKTYGRQYNDDLTHLVKAPNGYLAVGIS
                     NSGHDDNAWTLRLDENGDSLWSYNWGGNMGDGAMSAIAIDTGGFVVTGYYASTQTNGT
                     DGFLLLLNDSGVQVNLYTYGTGHYDEPHAIVPAIDGDGWVVCGHYGDVEADLNTHNVF
                     LEQIGRNGVKKWRKEFGGAMHDGGEDLVAGKNAYYIAGRSNSHAGTMEDVFYVKTDAG
                     GNLLDQQWFGTDADDEGYGICVGKEAVIICGASSGGPSGGKDIYLQQFGDVK"
     gene            complement(12375..12974)
                     /locus_tag="VFU15_00385"
     CDS             complement(12375..12974)
                     /locus_tag="VFU15_00385"
                     /inference="COORDINATES: protein motif:HMM:NF020463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbqC family protein"
                     /protein_id="HEU4716254.1"
                     /translation="MKEVLLPSVYAGPVSWYRAFFSTENVRIDVHENYVKQTWRNRCR
                     IAGPNGVQDLVVPVKTEGNHTAVKNVRIDYSESWQRKHWGAITSSYGKSPFFEFYSDR
                     FRPFYFQRDWEMLTSLNHELLRVTLEAFGVKKEFSLTQEFIPYAENDLRKIISPKHKN
                     EEMNSSRYVQVFGERHGFIPGLSVLDLIFCTGPDARNYL"
     gene            complement(12986..>13706)
                     /gene="lepB"
                     /locus_tag="VFU15_00390"
     CDS             complement(12986..>13706)
                     /gene="lepB"
                     /locus_tag="VFU15_00390"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEU4716255.1"
                     /translation="KPVNYPVDHRTHFVKRCVALPGDTLQIIDHEVYINGVLLPMPQK
                     VQLNYTVKTDTANFKKLHLEKLGLMREGREGKYEMLTMTLTQRQADSLRLVKEIISVD
                     ADISRAGLYDEQLFPHSESFPWNLDNYGPVVIPKKGTTVKLTVDSLPIYERIIVNYEH
                     NTMSVKNDSIFVNGKYTTSYTFRMNYFFMMGDNREYSMDSRYWGFVPEDHIVGRAVMI
                     LFSYDRKNGHVRWDRCFENIH"
BASE COUNT         3077 a         3890 c         3450 g         3289 t
ORIGIN      
        1 gaacgctggt tacaccggca actggtgggg cgttgtcgcg gatgcgtcag gacaaaatcc
       61 cattcaacag ggtccgaccg atccgtatcc cggcatgtac gtttcgacga cttcgctcgt
      121 gaattcgaat tacgccatcc cgaacccgct gcgttatgtg aattcagaaa ccgttccgtt
      181 catcgccata ccgacgaatg tgctcgcgtc aggaaatatc attgtcggtg atgtggcgta
      241 cgtttacaac accaccaccg ggcagtcctg cttcgcgata tacgccgatg cgggcaacac
      301 aaccagcatt ggcgaggcgt ccatcatgac ggcaagtctc gtcggcgtcg atcccgacgt
      361 gcgcaccggc ggaacatccc agggcatcat cgattatgtc gtgttcccgc attccggctt
      421 cgggcaagga tacattccca ccatcgcaca gatcgacagc atcggcaatc ttatgctggc
      481 aacggtcggc ggtccgtgca tcgcttcctg catcggcccg gtttacaaca accagcctcc
      541 ttccacagcg gtgaacacgc ctgcgggctg ggacacgaca tccttcacag caacattcag
      601 cgacataaat aacggttgca gcgcagggat cgaaaaaaca ttctaccagg tttccgatta
      661 caacacgaac aacgaatgga gagcgaacaa cgcaaatgga tttttcaatg acgacttcaa
      721 cggttccgcg atcagtcccg gctggacttc cgtggcagga acgtgggcga tcagcgggaa
      781 tgcgctgtat gaatcggatg aaaattcttc gaacacgaat atttacgcac cgctcgcgca
      841 aaacctgtca gactgttacc tctacgaatg gacaggcaca atggatggaa gcggcaacaa
      901 ccgtcgcgcc ggttttcatt tcttctgcga ccagccggat tcgagcaacc gggggaattc
      961 ctactttgtg tggttccgtc tcgatgacga caagatccag atctacaaag tcattaataa
     1021 ttcctggggc aacaatcccg tgctcgatac tgcgtacaat ttcacggcga accagtctta
     1081 tgatttcaaa gtgatgtacg accgcatttc gggcgtgatc cgcgtttatg tgaatgatgt
     1141 ttcctgcgca caatgggtgg attccctgcc tattgcgaac ggttcctacg tttcattccg
     1201 caacgggaac tgcaattaca aggtcgataa tttcaaagtc ttccgttcac gctcttcttc
     1261 tgcgctggtc agcgccggaa ccgggaacgg gaatgacatc cgttttgaaa accatgatcc
     1321 ggcgacaccc gcgggaagga tcagctccgt tgtgaccgac cggatgcgcg ggatcagcgc
     1381 ggtttcggac cagtatgtca atacggattt cacccatccg tcttctccgc ttgctgtcaa
     1441 tgacggaacc gcttccgata tcgacacgac attcaacgga acgcagctcg aagcgaactg
     1501 gagcgcgtgc gccgatccta attcccatat ccgcgcttac tattttgcca tcggaacaac
     1561 gccgggcgct tcggatgtga tcccgtggac cagcaacggc atcaatacat cggtcaccgt
     1621 aaccgggctg acgcttatgc accagcagca atattatttc agcgtgttgt cggaagattc
     1681 agcgatgatg cagtcggcag tgaccgtttc cgacgggcaa ttgtattttg acgtcacgac
     1741 agaagccggg cttcattccg cagacaacag ccagcctccc ctgctctacc cgaatccatc
     1801 accgggtgtt tttacgatcg gggggacttt cagcggcgat ttttcgatga cagttttcag
     1861 ccaggacgga aaaatggtga tgaatgaaac gcgggaagaa aacgagcgga acgtggacat
     1921 ttcttcccaa cccgacggta tctatttcgt ccgcattacc gcaggaggaa aaacacacct
     1981 tcaaaaagtg gtaaaagctg aataatcttt ttcgccgccg gatcataaaa tcctgcacac
     2041 attccaccac ttttcacagc gtacacgatc ctgagtcccc gcgtgttcat gctggtcccg
     2101 gcacgggata cagaaaacaa accatcatcg gttattgacc ttcttctccc tgaactcctc
     2161 gatgatcacc tgcatgatat caccgcggat gcccatctcc gcgagcctgt tcggtttggc
     2221 atcgggataa acacggttgg aaagaaaaac aaagacaaca cctgtttccg ggtcggccca
     2281 ggcctgcgtg ccggtgaaac cctggtgacc gaagctgcgc agtgagatgc tgtcgcaggc
     2341 gggatttcct ttttttacat cgggctccgg cttgtcaaaa ccgatcccgc ggcgatttcc
     2401 acttcccggg taggccacgc tcgtgaactg cgccaccgtc gccgaatcga aatagcggcg
     2461 tccgccgtac gtgccgtagt tgagatacag ctgcatcatc acgcccacgt cgttcgcatc
     2521 gctgaacacg cccgcgtgac cgccaattcc gccgagcatc gctgcgccct ggtcgtggac
     2581 atcgccctgc agctgctgct tgcgaaaatc cttgtcgttc tccgttggcg cgcagcgcga
     2641 gctgggaaaa cgaagacgcg gctggtaacc gatcgtcgtt aatccaagcg ggaaataaaa
     2701 attccggttc gcgtaatcgc tcagcagcat gtgcgattta cgctcgatga tctttttcag
     2761 gtaataatag ccgagatcgc tgtacacgaa tttgtgatcg tctttcaccg ggctttcatt
     2821 gatgcgcttg tagatcgtgt cgatgtaatt tttattcatg aacaatcccg gcgctacgta
     2881 aatggaaaac gtatcgctcc agcttttccg ccagatgctt ttgtcgtaat tgccgtcctt
     2941 gtcttccgtt ttcatccaga acgggatcca cgcctgcagg cccgcctggt gcgcaagcat
     3001 ctcgcggatg atgatgtgct ccttgttcgt ccctttcagc tcaggcaggt aatcgccgag
     3061 cgttttatcg agatcgatct cgcctttgtc tttcatcagc atgaccgcgg gaaccgttgc
     3121 catgatcttg gtgacggaag ctatgtcata gagatcgtcg ttcctcacca aatgcgaatt
     3181 cggatcgtag gtaaaacgtc cgtaggattt gtacaggaac actttcccgt ctttcgccgc
     3241 gaacacctgg cagcccggga acgctttcgc atccattcct tttttcacga tgtcgtcgat
     3301 cttcgcgagc gcgccgggac tgaaacccac ctcttccggc atggtgtatt tgaaacgggt
     3361 gggcgccggt gttgtcaacc cgctgcctgc tttccagtcg tttgtcgtca ccggaatttt
     3421 cccggatgcg ctcacgccgc cgaagatgat ctgcgcggaa atatcttcga ggtaatcctg
     3481 gttctcatac gaaagaatca ctgcatcaca gctttcagca cgtggcagga aattgagcga
     3541 atacgccggg ccgaacacgt cgagcacgac atgctttttc attttcagca gcgtgtcgat
     3601 cagcagaaca tccaggtcgc cgatgccgaa acccgcagca ggtttctgtg aaacgccgtg
     3661 cagcgaaaga acaacacagt tgaacgcctg caattttttc agcaccgtgt cgcgctccgc
     3721 cgttttgcaa tcgcgcatca gcgaaaaatc cgtcaccggc gcatacatgc cgaggcgttg
     3781 ctggaatacg accgaatcgt tcctgcccac cgccagcgaa gcgatcttca ttgtgtcgag
     3841 gtgcgtgaaa ggaatcagcg tgttgtcatt cttaagcagc gtaaccgctt tctccgccat
     3901 ctgccggcac aaccatgcgg cgccgggcgt attcagatct tcccacaggt ttttctgctt
     3961 cactgttttc tttttgtcga gaccgagcca ctttttcgct gcgagaattt tccggcaatg
     4021 tgcatccaca tcagcctgcg tcagctcgcc ttttgcgatc gatcccctga tcatcgccac
     4081 cgctttcgga acgtcacctg aaaaaagaag cacgtcgttt cccgcgagaa gcgctttcag
     4141 gtccacttcg ccgggcttgt agaactggct cacgcctttc atgttcagcg catcggtgaa
     4201 aacaagtccg cggaacccga gctgctcttt cagcaaaccg gtcaccacat ttttcgaaag
     4261 cgtcgtggcg agattcgtcg tcgtgtcgta cgcgggaatg tacaggtgcg cgaccatcat
     4321 gctgccgagc ccgttcctga tgagctgttt gaacgggaac agttccaccg aatccatacg
     4381 cgctttgtcg tggttgatga ccggcaaggt tttgtgcgaa tcgctgtcgg tatcgccatg
     4441 accgggaaaa tgttttccgc aggccatcac acctccgtcc tgcagacctt tcatgtacat
     4501 caccgctttg tgcgcgacca taaatttatt ttccccgaac gagcgcgtgc cgatgaccgg
     4561 attcgccggg ttgcagttca cgtcggcgac gggagaaaag ctcacgttca caccgagacg
     4621 tttgcactcg cgcgccatct cgatccccat tttgtaaacg agcgaatcgt cgtccatagc
     4681 gcccatcgtc atttcgcgcg gaaactgcac cgtgctgtcg aggcgcatgg aaagtcccca
     4741 ttccgcatcc atggcgatca tgagcggaat tttagaaagc ttctggtatt tattcgtcag
     4801 ctgcgcctgg cgcacaggtc cgccctgcat gaagatgagc ccgccgatgt gattttttgc
     4861 aatgaggtcg gtgatctctt tcgtgtgctt cgcgtctttg ttcgaccacg cagcgaccat
     4921 gaacagctgc ccgatctttt catcctgcgt cattgttttc atgacggaat ccacccaccg
     4981 gtccgtttca ttttccagga acggaggcgg cagcgtatcg ggaagcaggc tccgggaagg
     5041 gaaaacggtt ttcgggaaaa cgaaagaagc ggacgcctgt tcaccgccgg gataaaaggc
     5101 catcaccagc gggatgataa gagcggggag aaaaatcaat atggcgcgca gctccttcaa
     5161 cagctttttt cttcgcatga acattcgcta aaattaccgt caatgaccgt gcctgctttt
     5221 cagtcacggg gtaattttca acaaattaac tgttgatgaa ggaagttgtg ccacagaatg
     5281 agcaggtgta gtgtttttta atgcagctgt gcttttttcg ccggcggacc ttccggatcg
     5341 atgagatctg cgacagcttt cctggaaagc aacatggaaa atgccggagg ttcccggtta
     5401 tcgccgcgac cgcacctttt cattcgttcc cggaaagtac ggccgtttct tcctgtttcg
     5461 tatttgcttt ttttcacaaa cgccagcgaa atccattcgt aaacacgatt tttcactgta
     5521 atttcgaagc tccgaaaacg cttttgtgat gcaggacgtc atccgccttt tacccgattc
     5581 agtcgccaac cagatcgccg ccggtgaagt catccagcgg cccgcttctg ccgtgaagga
     5641 attgctcgag aattcggtcg acgcgggcgc cacgtccata aagctcatcg tacgcgacgc
     5701 aggccgcacg ctcatccagg tggtggacga cggtaagggc atgtcggaaa cggacgcgcg
     5761 tctttccttc gagcgccatg ccacgtcgaa gatccgccac gccgacgatc ttttcaacct
     5821 gcacacgatg ggtttccggg gcgaagcgct ggcttccatc gccgccatcg cgcacgtgga
     5881 gttgaaaacg cggcgggaag aagacgaggt cggaacggcg atcgtcatcg aaggcagcgt
     5941 gttccgttcg caggaaccgg tagctgttcc gccgggaact tccttctcgg tgaaaaatct
     6001 tttcttcaat gttccggcgc ggcggaattt tctcaaatcc gatgccgtcg agttccgcca
     6061 tatcgtggaa gagttcgaac gcgtcgcatt cgcgcacccg cagatcgcgt tctcgctcac
     6121 gcacaacggc aacgagctgt tcaacctgca gaaagggaat ttccggcagc gcatcatttc
     6181 gctgtatgga aacccataca acccgcgact cgttcccgtc gaggaaacta cggacatcgt
     6241 ttccgtgagc gggttcatca tcaagccgga gttttcgaaa aaaacacgcg gcgagcagtt
     6301 cttcttcctg aacaagcgct tcataaaaaa tccgtatctc caccacgccg tgcaggcggc
     6361 ttacgaacag ctgctcccgg ccgacacgta tccttcctat tttcttttcc tcgatgtcga
     6421 tccgaaaacc atcgacgtga acattcaccc gacaaagacg gaagtgaagt tcgaagacga
     6481 acgagccgtg tatgcgatca tccgctccac ggtaaaaaaa tcgctcgggc aatacaacat
     6541 cgctccctcg ctggattttg agcaggagat gagcatggaa attccgtacc gcaggcccgg
     6601 cgatcctgtt tcggctcccg gcattacggt ggacacatcc tacaacccgt tttccgacag
     6661 taagaacgga gcaggcggga aaggtcatac gcatgtcaca catttttcac ccactgaaaa
     6721 gagcaatctt tccaactggg aagaactgtt cactgcacac atgaatgccg gcgtgatccg
     6781 cagcgaaacg gaaaaagagg aaacaacagc cgaaagaaag aaagaagaat tttccgcgaa
     6841 gatctggcag ctgcatgaac gctatctcct ctctcccatc cgctcgggca ttcttgtcat
     6901 cgaccagcag cgggcgcacg agcgtgtgct gtatgaacgt tacctgaaaa tattttccgg
     6961 gcagaaaatg aattcacagc agcttctttt cccggagacc gccgaatttt caccttcgaa
     7021 cacggcgctg gtcagcgggc tgctcgacga gatcaaccag ctgggattcg atcttcgtcc
     7081 tttcggcagc aatacgttcg tgatccacgg cgttccaccg ggaagcgaag aaaaaaatgc
     7141 gctgcagatg ctcgaaggca tgctcgataa tttccggcag aacgaacagg agcttcatct
     7201 ggaaagagca gagaacctcg cgcgctcact ggcgaagcag gcggccgtaa aaccgggacg
     7261 cgccatgacc gcggaagaaa tgcaggcgct ggtggacgag cttttcgcct gtgaaacgcc
     7321 ttcgcatgcg ccggacggga aaccggcctt cattaatttc ccggtcgaag aaattgacaa
     7381 gcgattccgt cgctgaacga ttgtaccgga cgtgagctat taccatcaat ataagccaat
     7441 gcggcattcc cagtttccac cgggtgtgct gaacctcatc atcatcaacg cgatctttta
     7501 tttcgcgaag atgctcgtgc tatcctcgcg cggcgtcgac ctcgacggct atctcgggct
     7561 gttccacccg ttctcccccg ctttccactg gtaccagtac atcaccagcc tgttcatgca
     7621 cgccagcgta acgcacctcg tgatgaacat gttcggtctc tggctgttcg ggcacatgct
     7681 cgaaaatgct tacggcacca aacgtttcct cattttttat ttcgtcagcg gcgtcggcgc
     7741 ctcgatgatc ttccagctct ggtcttccat cctgcattac cagcagcttt ccggtttcgg
     7801 cagctttacc gatgtgatgc gcacctatcc gcccggcgac ctgctcatcg gcgcgtcggg
     7861 atgcgtctac ggcgtgctca tgggcgcggc cctgcttttc cccaatacgc agatgagctt
     7921 cctctttctt cccggcctcc cgatgaagct gaaatgggtc gctgtgctgt atggcggcgc
     7981 cgaagtttat aacgcgtggc aggcggcgcc cggcgacaac gtcgcgcatt ttgcgcacgt
     8041 gggtggaatg atttttggat tcatcctcgt gaagatctat tcgagggacc ggacacattt
     8101 ttactgagta tgagcattgt tgcagacata agaaattcgt tccgcggaac gtatgcgggc
     8161 ctgatgcggc tcatcgtcat caacctcggc gtattcatcg tgctgaatgt gatcgtcgag
     8221 atcatgcgca tttccatgac gccggatgcg gaagagctcg ttgcgcagta tttcgttctt
     8281 ccctcttccc tgcccgacct tttcccgcat atatggacgc tcatcacgta tatgttcacg
     8341 catttcggct tcgggcatct cttcttcaac atgctctggc ttttctttct cgggaaatta
     8401 ttctgcgaac agctgggacc gaaacggatc atcggcactt acgttttcgg cggcgttatc
     8461 ggcggacttc ttttcgtcct cgtctgcaac cttgttccgg cgttccgcta ctccagcctc
     8521 gaaggggcat cggcgggcgt gatggccgtg gtggtgacga tcgggacgta tgcgcccaac
     8581 cttcgcatga ctatttttcc tttccgtttc gaagtgcagc tgaaatggat cgtgctcgtc
     8641 gcattcctgc ttacgtccgt gatctatctt tcggaaaaca cgggcggaaa agtggcgcac
     8701 atcggcggag cgctgttcgg ttttatctac ggctggcagc tgaaaagagg gagaactttt
     8761 ttcgacggca tcacgcagct gctttcgttc aaacgttcga agctccgcgt cgagcaccgg
     8821 cgttccattt ccgatgaaca atacaatttg cagaagatga acgtcaaaaa acgcatagac
     8881 gaaattctcg acaagatctc ccgctccggt tacgacagcc tcaacaggga ggaaaaagat
     8941 ttcctccgca aataccacga taagctataa ccgacctttt tccacatccg tttgaaaaac
     9001 gacaacggtg aaaccgaaag caagaccgcg cctcccgcac gaaaaaaggg aaggatcgcc
     9061 aatgcggtca actggacgtt catcctgctg ctcggactct cgtgcctcgc gccgttcgta
     9121 aatcctgccg tgttctggcc gcttgctttt tccgggcttc ttttcccttt gttcttcctg
     9181 gtaaatgcgc tcctgcttct ctggtggacg atccggcgaa aacggcgggg cttttattcc
     9241 ctcgccatgt tcctgctcag tctcccgatg tatttccggc aggcagccgt tttcggaacg
     9301 aaagaaaaat cgccgccggg cgccgtgaag atcatgagct acaacgtgaa gctgttcgac
     9361 ctgtacaact ggacgggcaa ccgggaatcg cgcgcagaga tattttcatt gctgaaagcg
     9421 cagcaaccgc agctgctctg cctccaggaa tttttcaacc aggacaccgg gcgtttccgc
     9481 aatctcgatt cgctgaaaaa gctgctcgcg ctgccttatg cgcacacgga atacacgatc
     9541 tcgctgcacg gtgacgatca ctggggaatg gcgacgttca gtcaattccc gatcgtgaac
     9601 gagggaaaga tcgtcttcaa caaccggagc aacaacatct gcatttacac cgacatcctg
     9661 atgaacggcg atacggtccg ggtttacaac atgcacctgc agtcgatcag cttcggttac
     9721 gccgacctga aatttctccg ggaaatgtcg aaagccgaac aatccgccga cgagctcgaa
     9781 aacagccgca acatcctgcg ccggatgaaa cgcgcttata tccgccgcgc gaaccagtcg
     9841 caggccgtgg ccgatcacat cgcgcaatgt ccttacccgg ttatcgtgtg cggagatttc
     9901 aacgatacac caacttcata cacataccgc acgatcggga aaaacctgga agattctttt
     9961 ctcgaatcgg gaagaggttt cgggaaaacg ttcatcaatc cgttcccgtt cccgcgcatc
    10021 gattacattt tccattcatc gcaattttcg acgtggcagt tcaatgtcat ccgcacggat
    10081 aacctgtccg atcattaccc ggtggaatgc atgatcagga agaacaaatg atcagggaag
    10141 aatggccggg cgctgcgtga tctcgtggta gcagatgatg tctcctttgc agaaataatg
    10201 cgtgaccagg cttttgcgcg acgcattttc atccgtcacc ggttcaccgc catgcagcag
    10261 gttcgcgtgc cacacgagca ggtcgccttt ctttgcgagg aatatttcct tctgtaattt
    10321 tttttcactc acgagctccg cgatcttttt ctcgtaattg ccgtacaggt catcgccgat
    10381 cacgagcgca ttgctggaat gatcgaagtc ttccccgaga atgtaaggta gcctgtggct
    10441 tcccgggaaa taatgcagcg gtccgccgga agctgaaata tcttcgagcg ccatccacgt
    10501 cgccgcaaga taaccgacgg gttcggtggt catgtggatg aaatcggaat gcgttttctg
    10561 ccggcttccc ttgaggaaac tgatggtctg gaagggaacg gtttcgcgtc ccagcgtgaa
    10621 ggaaaggaat tctttcagca gccggtcttt tgtgatcgcg cggatcgcgg gtgaatgttc
    10681 ccatgcgttc atcacgcgcg agttcgtgta atcgaaatcg accttctttt cccggatcag
    10741 ctcatccagc tcgctgttga tgcggtcgca ggtttcgccc gaagcaaacc cttccaggat
    10801 cataaatccc cgcgcaggcc atttctgcaa ttcttcctgc cactgcaatg ggaatttttc
    10861 gagtcccggg tttcccgcca gcattttttc cgcatccggc cggtcgagcc acggaatttc
    10921 cgccccgggt tttgagatat gcgcgtgcga aatggatccg acaacgctct tgctgatccc
    10981 gtatttgcgg taaagcgttt tatttttttt cagcttcccg aaatgcgtca tgttgtgaag
    11041 aacatgcagt atgcgcattc ttcgcagcgt ggcccagtat tttttcagga atgacatttc
    11101 cggcaaagat aacactccgg caatagttca gcttgcgtaa taagcatctc ccttctctca
    11161 cgttcaatac ccacgcctca cttcacatct ccaaactgct gcaggtaaat gtcttttccg
    11221 ccggaaggtc cgccggaaga cgcgccacag atgatcacgg cttctttccc aacgcaaatg
    11281 ccgtatcctt catcgtcagc atccgtgccg aaccactgct gatcgaggag atttccgcct
    11341 gcatcggttt tcacatagaa cacatcttcc attgttccgg cgtggctgtt gcttctgcct
    11401 gcgatgtagt aggcattttt ccctgcgaca agatcttccc ctccgtcatg catagcgccg
    11461 ccgaattcct tccgccattt ttttacaccg ttgcgcccga tctgctcgag aaaaacattg
    11521 tgcgtgttaa gatccgcttc cacgtcgccg taatgcccac atacgaccca gccatccccg
    11581 tcgatcgcag gaacgatcgc gtgcggttcg tcatagtggc ccgttccgta tgtgtagagg
    11641 ttcacctgta cgccggaatc gttcagcagg agcaggaaac cgtccgtacc gttcgtctgc
    11701 gtgctggcat aatacccggt caccacaaat ccacccgtat cgatcgcgat ggccgacatg
    11761 gcgccgtcgc ccatgttgcc gccccagttg tagctccaga gactgtcccc gttctcatcg
    11821 agacgcaggg tccatgcgtt gtcatcatga ccgctgtttg aaatcccgac cgcgagataa
    11881 ccgttagggg ctttcacgag gtgcgtaagg tcatcgttgt actggcggcc gtatgttttc
    11941 gaccagatca catttccggc tgtatccgct ttgatgatcc agaaatcgtc tgcacccgcg
    12001 ccctggctcg aagttgtcgc tcccagcaca acgttcccgt cgtcgcagag atcgaccgac
    12061 cagcattgct cgtttccggc gccaccgtaa tacttcatcc atttctggtt accgtgcatg
    12121 tccgttttca gaaggataac atcatttcct ccgttgggac ccagcccgta accggcgatc
    12181 atcaggtcgc cgccgggaag acgaagcact ttccgcgcca tctcatcacc ggaagtgccg
    12241 taataccgga caaaattttc aggaatataa acctcgtcgt ccttcctgca ggaaaacatc
    12301 agcacgaaag gaagaaggaa aatgaatgct tttttcatcg ctggaacgcg ggacgaaaaa
    12361 ttgatcccgt taaattaaag ataattccgg gcatccggcc cggtgcagaa aattaaatcg
    12421 agaacggaaa gcccgggaat aaatccgtgg cgctcaccga aaacctgcac gtaccgggaa
    12481 ctgttcatct cttcattttt atgcttcggc gaaatgatct tccgcaggtc gttttcagca
    12541 tacgggataa attcctgcgt cagtgaaaat tcttttttca cgccaaacgc ttccagcgta
    12601 acgcgcaaca gttcatggtt gaggctggtc agcatctccc aatcgcgctg aaaataaaat
    12661 ggacggaaac gatccgaata gaattcaaaa aaaggcgatt tcccatagga agatgtgatc
    12721 gccccccaat gcttgcgctg ccagctttca ctgtagtcga tgcgcacgtt tttcacggcg
    12781 gtgtgattgc cttccgtctt cacgggtaca acaagatcct gtacaccgtt cggtccggca
    12841 atgcggcaac ggttacgcca ggtctgcttc acgtaatttt cgtgcacatc gatccgcaca
    12901 ttttccgttg aaaaaaaagc ccggtaccag ctcacaggtc ctgcataaac ggaaggaagc
    12961 agcacttctt tcacagcaaa aaacttcagt ggatgttctc gaagcagcgg tcccaccgca
    13021 catgcccgtt cttcctgtcg taggaaaaaa ggatcatcac ggcccggcct acgatatgat
    13081 cttccggaac gaagccccag taacgtgaat ccatggaata ttcgcggttg tcgcccatca
    13141 tgaaaaaata attcatccgg aacgtgtacg acgttgtgta ttttccattg acgaagatgc
    13201 tgtcgttctt cacgctcatg gtgttgtgct catagttcac gatgatgcgc tcataaatcg
    13261 gaagactgtc gacggtaagc tttacggtcg tacctttttt cggaatgacc acgggaccgt
    13321 aattatcgag gttccacggg aacgattccg aatgcgggaa caactgttcg tcgtaaagtc
    13381 ccgcccgcga aatatcggcg tcgacggaaa ttatttcttt cacaaggcga agactgtctg
    13441 cctgccgctg cgtcagcgtc atcgtgagca tttcgtattt gccctcgcgg ccttcgcgca
    13501 tcaggccgag cttttcgagg tgaagctttt tgaaattcgc cgtgtctgtt ttcaccgtat
    13561 aattcagctg caccttctgc ggcatgggca gaagaacgcc attgatgtac acttcatggt
    13621 cgatgatctg cagcgtatcg cccggaagcg ccacgcatcg cttgacaaaa tgcgtgcggt
    13681 gatcgacggg ataattcacc ggtttt
//
LOCUS       DASTSF010000014        12435 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1771133,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000014 DASTSF010000000
VERSION     DASTSF010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 12435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12435)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12435
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1771133"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..603
                     /locus_tag="VFU15_00395"
     CDS             <1..603
                     /locus_tag="VFU15_00395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716256.1"
                     /translation="DNELGVRPYFRMGTFVYAYDENGKLVSRHFLDRVNGVILVSDSL
                     AYKTDEKDANVLHISHLFRISGVTGWLTLDAQMWNKADNLLLTWDYGKSAGVDHYEYY
                     GNGKLSKITFAEQSGQLVYTDTYCRTGTRLDSLVSVTYSANGKKRTGRKEYEFVFVYD
                     AGGMISKVTDYEYVLPVKKSRNRKKPLLDSVYETSYSPLQ"
     gene            677..1672
                     /locus_tag="VFU15_00400"
     CDS             677..1672
                     /locus_tag="VFU15_00400"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018477041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEU4716257.1"
                     /translation="MNRILLIQTAFTGDVVLATPLIEKLHRFYPGAKIDFLLKKGNEG
                     LLTGHPLLDEVLIFDKKNGKLKNLFSLLRRIRRKKYDVVVNMHRFGSSGFLTAFSGAE
                     EKIGFDKNPWSRFFTKRIAHEIGNGKHEVRRNLELIQHLTDSQPERPKLYPSEADYKH
                     VSFHKSSGKYVCMAPTSVWFTKQWPEEKWVELIKLVPREFRIFLLGAPSDNKTCEDIL
                     RNSRHGNIANLAGHLSFLQSAALMESASMNFVNDSAPMHFASAMNAPVTAIFCSTVPA
                     FGFGPLSDKSFVAETKEKLSCRPCGLHGYKACPEGHFRCALEIDPAEVVKNLSGR"
     gene            1708..2775
                     /locus_tag="VFU15_00405"
     CDS             1708..2775
                     /locus_tag="VFU15_00405"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019597009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HEU4716258.1"
                     /translation="MENKKFNYELFFHLSPDLLCIAGFDGYFKKINPAVSAVLGYTEE
                     ELLARPVNDFVYGEDKIATERVREELRRSKPLYNFENRYLTKTGEIVWLSWTSFPVPE
                     DKLVFAVAKNITHKKKLEAERNVHLANLTMANKDLKQLSFTTSHDLRSPVNNLLSLLD
                     MIDVFRVADPETLELLEILKLSGSRLKQTLNNYVDNLSEQHRLHARVEETDLSECLHE
                     VVQSIRSLIDTSKATVHTDFSRLNKIMFNRTYLKSIFLNLITNSIKYARPDVFPVISI
                     SSEKTDDTVKLVISDNGRGFDMAKVKDRIFGLHQKFHDHNESKGIGLYLVYSHVTDLG
                     GQITVESGVNEGAKFTITVRT"
     gene            2836..4314
                     /locus_tag="VFU15_00410"
     CDS             2836..4314
                     /locus_tag="VFU15_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017733639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEU4716259.1"
                     /translation="METEKFLAHPVFKIISGEAARLGVDAYVIGGYVRDSILGRPCKD
                     IDVVAVSHDPEIKAVGILLAEAVAKKLSVHVHVFRNFGTAQIKLDDWDVEFVGARKES
                     YSRDSRKPVVEDGTLKDDQDRRDFTINALAINLGEKKYGELLDPFGGRADMEKKIIRT
                     PLDPGITFSDDPLRMMRAIRFATQLDYTIEDKCLASIKENAPRLKIVSQERITDELNK
                     IIASKVPSVGFKILFDTGLLKIFFPQMCALHGVEIVNGKAHKDNFYHTLQVLDNVAAK
                     SDNLWLRWSAILHDIAKPATKRFEPEHGWTFHGHEDRGARMVPKIFAALKLPLSEHMK
                     FVQKLVQLHLRPIVLSKEEVTDSAVRRLLFDAGDDVDELMILCECDITSKQETRVKRY
                     LKNFEIVRRKLKEIEEKDKVRNWQPPVTGEDIMKALNIAPSLQVGIIKTVIREAILDG
                     KIPNERAAAWELMIGEAKKSGLHVQPGYETPNPETLPVKKEE"
     gene            4383..5249
                     /gene="prmC"
                     /locus_tag="VFU15_00415"
     CDS             4383..5249
                     /gene="prmC"
                     /locus_tag="VFU15_00415"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR03534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEU4716260.1"
                     /translation="MKIPSNKVRDIVQYFRHELSALYEKEEIEKFIEFCFFDFVRMTK
                     SDLLLKGESTVTESDLLRFHFAVKDLKRGKPVQYILGKAWFYGMEFSVTPDVLIPRPE
                     TEELVQLVVEEAKAHPETFSIADIGTGSGCIAASLKKKIPRAHVYAVDVSEPALAVAR
                     ENAQKLETEIAFLHLDILRAEEQGKIPSVMFIVSNPPYVKNSERSSMQKNVTDYEPHT
                     ALFVPDEDALVFYRALAALGKAKLKPGGKIFVEINEALGPETCILFQKSGYPDVQLKQ
                     DLRGKDRFVVAG"
     gene            5375..6121
                     /locus_tag="VFU15_00420"
     CDS             5375..6121
                     /locus_tag="VFU15_00420"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017729777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU4716261.1"
                     /translation="MKISVITACYNNAGTIADTIASVLSQDYTDLEYIIVDGASTDGT
                     MEMVRANEKRIAKIVSEKDDGIYFALNKGIDLATGDVIAFLHADDVYASPSVLSDVMK
                     VFAEKKTESVYGDLVYVDRNDLSKITRRWFSGEYHDGIFRRGWMPPHPSFFLLKSCYE
                     KFGKFDTSFRTAADYELMLRMLHRHKISTAYLHEVIVKMRVGGVSNVSLKNRIRANRE
                     DRRAWKVNGLRPGFLTLWWKPLSKVLQFFS"
     gene            complement(6151..7119)
                     /locus_tag="VFU15_00425"
     CDS             complement(6151..7119)
                     /locus_tag="VFU15_00425"
                     /inference="COORDINATES: protein motif:HMM:NF039345.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6089 family protein"
                     /protein_id="HEU4716262.1"
                     /translation="MRKATPFVKTALLASSLFLLPFIAHAQYYWDIGISTGASNYLGD
                     MGGKQLTRRDFVSDMKMSQTHMTTTGLVRYKVNPFFSVKADIGWARISGDDRLSSNPG
                     RNGRNLSFRNDLFEAAAQAQFCFYEINDLGRTFKHKNSFRAYVGLGAGMVYNNPKALY
                     NGNYVALRPLMTEGKRYTAVTGIIPASMGFYFTMNKQWRIGWDFTWRTTFTDYLDDVS
                     TVYADPSTLSSATAAALANRTHEKTNIDPAFAENFTPGSKRGDPTHNDSYLTTSIDLS
                     YVMRTKSDWEKQHATWLHNRPGKFDNDHRYQQILWWHVKRKVRIVF"
     gene            complement(7279..8229)
                     /locus_tag="VFU15_00430"
     CDS             complement(7279..8229)
                     /locus_tag="VFU15_00430"
                     /inference="COORDINATES: protein motif:HMM:NF039345.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6089 family protein"
                     /protein_id="HEU4716263.1"
                     /translation="MKKLLFAAVIVLTPFIARAQYFWDFGVGVGGSNYLGEMGGKQLT
                     RRDFVMDIKMKETRQAVNGFARYKISPQFSIKGNLSYACIRGADSLCTNEPRYWRNLN
                     FRNQMFELSAECQYFFYEINDLGHTYRYRNNFRAYIGLGIGGLYHNPKGNFNGTWIAL
                     RPLETENVRYTKVTLTIPATAGFYFTVNKHYRIGWCIDWRTTFSDYLDDASTFYVQNT
                     VPGAAAVADRTNHAAANAWGAQNSNPGFGNNFGYQTWTDSDGNVHLNKRGDPTHNDSF
                     ITTSVEFSYVIRGKSSIYKSHYSYMFKGKKYKHRKVRAKF"
     gene            8336..9292
                     /locus_tag="VFU15_00435"
     CDS             8336..9292
                     /locus_tag="VFU15_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016649315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HEU4716264.1"
                     /translation="MKNRITSLFNIEFPVIQAGMIWCSGWELASAVSNAGGLGLIGSG
                     SMYPDVLRGHIRKCRAATAKPFGVNVPLLYPDIEKHMDVIISEGVKIVFTSAGNPKTW
                     TPLLKQNGITVVHVVSNVKFAVKCQEAGVDAVVAEGFEAGGHNGREETTTMVLIPLVR
                     NAVSIPLIAAGGIASGRQMLAAMALGAEGVQIGSRFVASVESSANENFKHAVTDAKEG
                     DTIVTMKQLVPVRLIKNKFYKAIEEAELRGAGAEELKAILGRARAKKGMFEGDMEEGE
                     LEIGQVSAMVREIKPAAEIMKEIVAEYEAVKNELCTTDQSPA"
     gene            9434..10678
                     /locus_tag="VFU15_00440"
     CDS             9434..10678
                     /locus_tag="VFU15_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015807417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEU4716265.1"
                     /translation="MIKFDRFTLTNGLRVIVHHDEATPLTCVNILYDVGARDEEESRT
                     GFAHLFEHLMFGGSVNIPNYDEPLQRVGGENNAFTSNDITNYYLTLPAENLETAFWLE
                     SDRMLSLAFSEKSLEVQRSVVIEEFKQRYLNQPYGDVWLLIRPLAYKVHPYKWATIGK
                     EIRHIEEAKMEDVKAFFGKHYNPSNAIMVVSGKVTTENVKALAEKWFGPIPPGKKPVR
                     DLPAEPEQNEYRRETVVKDVPLDALYMTFHMGERKDSEYYVMDIVSDILSRGNSSRMY
                     NRLVKEERIFTEVSAFVMGDLDKGLFIVSGKISPGVPVERAEDAVFAELQKLVDEKTG
                     ERELTKVKNKIESSMQFGEMNILDKSMNLAFFELLGDAAMIDREVEKYNAVTAEEVQR
                     TAAKILRKTNCSSLFYKSARKN"
     gene            10682..11962
                     /locus_tag="VFU15_00445"
     CDS             10682..11962
                     /locus_tag="VFU15_00445"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEU4716266.1"
                     /translation="MSIDRTTAPEFHTIGSIDIPRAERVKLSAGNDLWLLDAGTQDVL
                     RLDFIFNAGSRFQQKTLVASGVNEMLDEGTAQRSAEEIAEELDYFGAFIESEPGKDTS
                     SFTVFTMNRHLEPVMKIVSEILCGASFPENEFRVYIDNKKQKFIVDSDKVSVLARRKF
                     GEIIFGEKNPYGAAVKLTDFDAIGRKDLDAFHKTFYAKENCTIVASGKIPQGLEALLE
                     KYPAGNPGKKAAADDFAPFSPSAERIHTVEKAGAIQSAIRMGRPLFNKTHPDYHAMLV
                     LNTVLGGYFGSRLMSNIREDKGYTYGIGSGIMSMYNAGYFVISTEVGAEVTNAALEEI
                     YSEIARLQNDLIPEEELELVRNYMTGVFLRSTDGPFALGDRLKGLLGYGLGYDYYERY
                     LETIRSVSANELRELAQKYLRKEDIFELVAGVRK"
     gene            12010..>12435
                     /locus_tag="VFU15_00450"
     CDS             12010..>12435
                     /locus_tag="VFU15_00450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716267.1"
                     /translation="MRTPFYIALILLLASFAAGKTEKKYDPAKLFTVKQLQEDFTALR
                     ARLERKDPNLYLYTPKKRLDFVFDSLYSRIEAPMTAMDFFRLLAALRSEIRDGHTYPM
                     ASREMENGFFNKDSVFLPYLFVYLRHKLYVRRSFTPDSAI"
BASE COUNT         3067 a         3105 c         3496 g         2767 t
ORIGIN      
        1 gataacgagc tgggcgttcg tccctatttc aggatgggaa cgttcgtgta cgcatacgat
       61 gaaaacggga aactggtgtc gagacatttc cttgatcgtg tcaatggcgt cattctcgtt
      121 tctgattcac tcgcgtataa aacggatgag aaggatgcca acgtcctgca tatttctcat
      181 ttattccgga tttcaggcgt cacgggatgg ctgaccttgg atgcgcagat gtggaataaa
      241 gcggacaacc tgttgctcac atgggattac ggaaaatcgg ccggggtcga tcattatgaa
      301 tattatggaa atggaaagct gtcgaagata acgtttgcgg aacagtcggg acagctggta
      361 tacacggata cctattgcag gacgggtaca cgcctcgatt cattggtttc ggtgacgtat
      421 tccgccaatg gaaaaaagag gacgggaaga aaggaatacg aatttgtttt tgtttatgat
      481 gccggtggaa tgatcagcaa agtgacggat tacgaatatg tactgcctgt aaaaaagtcc
      541 cggaacagga aaaagccgtt gctcgattct gtctatgaaa cgtcgtattc tcccttgcaa
      601 tgatccggcg cattccccgt taataccagg aataatccgc gtttcattcg gacagcgcgc
      661 ttaactttgt ttttcattga accgcatcct cctcatacaa accgctttca ccggcgacgt
      721 cgttctcgcg acgccgctca ttgaaaagct gcaccgtttt tatcccgggg cgaagatcga
      781 tttcctgctg aaaaaaggaa atgaaggttt gcttaccggt cacccgctgc tggacgaagt
      841 gctgatcttc gacaagaaga acggcaagct gaagaatctt ttttcattgc tgcgccgcat
      901 acggcggaag aaatatgatg tcgtggtgaa catgcatcgc ttcggttcgt cgggatttct
      961 cactgcgttt tccggtgcgg aagagaagat cggtttcgat aaaaatccct ggagccgttt
     1021 ttttacgaaa cgcatcgcgc acgagatcgg caacgggaaa catgaagtgc gtagaaacct
     1081 ggaattgatt cagcacctca ccgattcgca gccggaacgg ccaaagttgt atccctccga
     1141 agcggattac aaacacgttt ctttccacaa aagttcgggc aaatacgttt gtatggcgcc
     1201 cacgagcgtc tggttcacga aacaatggcc ggaggaaaaa tgggtggaac tgatcaagct
     1261 ggtgccccgt gaattccgca ttttcctgct cggcgcgcct tcggataata aaacctgcga
     1321 ggacatcctg cgcaacagcc ggcacgggaa tattgccaac ctggccgggc atctttcgtt
     1381 cctgcaatcg gccgcgctga tggaaagcgc atcgatgaat tttgtcaatg attccgcgcc
     1441 catgcatttc gcgagcgcga tgaatgcacc ggtgaccgcc atcttttgct caacggttcc
     1501 tgcgttcggt tttggtccgc tatcggataa aagcttcgtg gcggaaacaa aagaaaaact
     1561 ttcctgccgc ccttgcggac tgcacggtta taaagcctgc ccggaaggac atttcagatg
     1621 cgccctcgaa attgatcctg cagaagttgt aaaaaatctg tccggcagat aatttagcgc
     1681 cgcagttgtc gttactcata atcttcaatg gaaaataaaa agtttaatta cgagctgttt
     1741 ttccatcttt cacccgacct gctctgcatt gcaggttttg acggttattt caaaaaaata
     1801 aatcccgctg tttctgccgt gcttggctat acggaagaag aattgcttgc ccgtcccgtc
     1861 aatgattttg tttacggaga ggacaaaatc gcaaccgagc gtgtgaggga ggaactgaga
     1921 cgttcaaagc cgctttacaa tttcgagaac cggtacttaa cgaaaaccgg cgaaatcgtg
     1981 tggctttcgt ggacgtcatt cccggttccc gaagataaac tcgtgttcgc tgttgccaaa
     2041 aacatcacgc acaagaaaaa gctcgaagcg gaacgcaatg ttcacctggc gaacctgacg
     2101 atggcgaata aagacctgaa gcagttgtca ttcaccacct cgcacgacct gcgctcgccg
     2161 gtgaacaacc tgctttcgct gctggatatg atcgacgtgt tccgcgtggc ggacccggaa
     2221 acgctcgagc tgctcgagat acttaaactg tcgggcagcc ggctgaagca aacgctgaat
     2281 aattatgtcg acaacctgag cgagcagcat cgcctccatg cacgtgtgga agaaaccgac
     2341 ctgagcgaat gtcttcacga agtggtgcaa tccatccgct cgctgatcga cacgtcgaaa
     2401 gcgaccgtcc acacggattt ttcccggctg aacaagatca tgttcaacag gacgtacctg
     2461 aaaagcatat ttctcaacct catcaccaat tccatcaaat acgcccggcc ggacgtgttt
     2521 cctgttatca gcatctcatc cgaaaaaacg gacgatacgg taaagctggt catttctgat
     2581 aatggccgcg gttttgatat ggccaaagtg aaggacagga tattcgggct ccaccagaaa
     2641 ttccacgatc acaacgaaag caaagggata ggtctttacc tcgtttacag ccacgtgacg
     2701 gatctcggtg gacagatcac tgtcgaaagc ggggtgaacg aaggagcgaa gttcaccatc
     2761 accgtccgta cctgaagcct ctccgcgcat ctgccggtgt ttttccaaac gaagcatctt
     2821 ttacctttgc cgcgaatgga aacagaaaaa tttctcgccc atcctgtttt taagatcatt
     2881 tccggtgaag ctgcccgtct cggcgttgac gcctacgtga tcggtggtta cgtgcgcgac
     2941 tcgatcctcg gacgtccctg caaggatatc gatgtcgttg cggtcagcca cgatcccgaa
     3001 ataaaagccg tggggatcct gcttgccgaa gccgtggcga aaaaactttc cgtgcacgtt
     3061 catgtgttcc gcaatttcgg aacggcgcag atcaaactgg acgactggga cgtggaattc
     3121 gtcggtgcac gaaaagaatc gtacagccgc gattcgcgca agcccgtggt ggaagacggt
     3181 acgctcaagg acgaccagga ccgccgcgat ttcacgatca atgcgctggc gataaatctc
     3241 ggcgaaaaaa agtacggcga attgctcgac ccgttcggcg gaagggccga tatggaaaag
     3301 aagatcatac gcactccgct cgatccgggc atcacatttt ccgacgatcc gctgcgcatg
     3361 atgcgcgcca tacgcttcgc cacgcagctc gattacacca tcgaggacaa gtgtcttgct
     3421 tccataaagg agaacgcgcc gcgcctgaag atcgtttccc aggaacgcat caccgacgag
     3481 ctgaacaaga tcatcgcgtc gaaagttcct tcggtcggtt tcaaaattct tttcgatacc
     3541 ggtctcctga aaattttctt cccgcagatg tgcgcattac acggcgtcga aattgtgaac
     3601 gggaaagcgc acaaggataa tttttaccac acgctgcagg tgctggacaa tgtcgccgcg
     3661 aaatcggata acctctggct gcgctggtcc gccatcctgc acgacatcgc gaagccggcg
     3721 accaagcgtt tcgaaccgga gcacggctgg accttccacg ggcacgaaga ccgcggcgca
     3781 cgcatggtgc cgaaaatatt tgccgcgctc aagctcccgc tcagcgagca catgaaattc
     3841 gtgcagaaac tcgtgcaatt gcatctccgc ccgatcgttc tttcgaaaga ggaggtgacg
     3901 gattcagccg ttcgccgcct gctgttcgat gccggcgacg atgtcgatga actgatgatc
     3961 ctctgcgaat gcgacatcac ctcgaagcag gaaacgcggg tgaaacgtta cctgaagaat
     4021 ttcgagatcg tgcgcaggaa gctgaaggaa atagaggaga aggacaaggt gcggaactgg
     4081 cagccgccgg tgaccggcga ggatatcatg aaagcactca atatcgcgcc ctcgctgcag
     4141 gtgggaatta tcaaaaccgt gatccgcgaa gcgatcctcg atggaaaaat tccgaatgaa
     4201 cgcgccgccg cctgggaact gatgatcggt gaagcgaaaa aatctggatt gcacgtgcag
     4261 cccggctatg aaacgcccaa cccggaaacg ctgccggtga aaaaggaaga atgaaaaaat
     4321 cctggtcccg gtccgctcct gcagattagc cggcgcagcg accggaaaaa acgaaaacag
     4381 cattgaagat cccctccaat aaagtccgcg acattgtgca gtatttccgg cacgagcttt
     4441 cggctttgta cgaaaaagaa gagatcgaaa agttcatcga gttctgcttt ttcgattttg
     4501 tgcggatgac gaaaagcgac ctcctgctga aaggtgaaag caccgtcacg gaatccgacc
     4561 tgctcaggtt tcattttgca gtgaaagacc tgaaacgggg aaagccggtc cagtacatcc
     4621 tgggaaaagc gtggttttac gggatggaat tttcagtcac gcccgacgtg ctcattcccc
     4681 gcccggaaac ggaagagctg gtgcagctcg tggtggaaga ggcaaaagcg cacccggaaa
     4741 ctttttcaat tgcggatatc ggaacgggat ccggatgcat tgcggcttcg ctgaaaaaga
     4801 agatcccgcg cgcccatgtg tacgccgtgg acgtttcgga accggcgctt gctgttgcga
     4861 gggaaaatgc gcagaagctg gaaacggaga tcgcgtttct tcacctcgat attctccgcg
     4921 cggaagagca ggggaagatt ccctccgtca tgtttatcgt cagcaatcct ccttacgtga
     4981 aaaattcgga acgcagctcc atgcaaaaga acgtgacgga ctatgaaccg cacaccgcgc
     5041 tttttgttcc cgacgaagat gcgctcgttt tttatcgcgc gctggcagcg ctgggaaaag
     5101 caaaattaaa accgggtgga aaaattttcg tggagatcaa cgaggcgctc gggccggaga
     5161 cgtgcattct tttccagaaa tccggttatc ccgatgttca gctgaagcag gacctgcggg
     5221 gaaaagaccg tttcgtggtg gccgggtgag gaagttcatt gcgtgaaata atggaaggat
     5281 tgcccgtcat acctgcacac gccgttcatg gtgccggcca tttgaattcc tgcggaccca
     5341 ttctgatttg cgctgtatct tcgcaggaac gaacatgaaa atttccgtca tcaccgcctg
     5401 ctacaacaac gccggtacga tcgccgatac gatcgcatct gttctttccc aggattacac
     5461 cgacctggaa tacatcatcg tcgacggcgc ttcaacggac ggtacgatgg agatggtccg
     5521 tgcgaatgaa aagcggatcg cgaagatcgt cagtgaaaaa gacgacggaa tttattttgc
     5581 gctgaacaaa gggatcgatc ttgccaccgg cgacgtgatc gcgttcctgc atgcggacga
     5641 tgtgtatgca tcgccttccg tgctgtccga tgtgatgaag gttttcgcgg agaaaaaaac
     5701 ggaaagcgta tacggcgatc tcgtttatgt cgaccgcaat gacctctcga agatcacgcg
     5761 gcgatggttc tccggcgaat accacgacgg tattttccgc agaggctgga tgccgccgca
     5821 tccgtcgttc ttcctgctga aaagctgcta tgaaaaattc gggaagttcg acacgtcgtt
     5881 ccgcactgcc gccgattacg agctcatgct gcgcatgctg caccgccaca aaatttcaac
     5941 ggcatacctc catgaagtga tcgtaaaaat gcgcgtcggt ggcgtaagca atgtttcgct
     6001 gaagaacagg atacgcgcca accgtgaaga ccgccgcgcc tggaaggtga acggactgcg
     6061 tcctggtttt ctgacgctct ggtggaaacc gctttcgaaa gtgctccagt ttttcagctg
     6121 aaaaaaaacg ccccggaggg cgctgaaggt tcagaaaacg attcttactt ttcgtttgac
     6181 gtgccaccag aggatctgct ggtagcggtg gtcgttgtcg aattttcccg ggcggttgtg
     6241 aagccatgtg gcatgttgct tttcccagtc gctttttgtg cgcatcacgt agctgagatc
     6301 gatgcttgtc gtgaggtagg aatcgttgtg cgtgggatcg ccacgcttgc tgccgggggt
     6361 gaagttttcc gcgaacgcgg gatcgatgtt tgtcttttca tgcgtccggt tcgcgagcgc
     6421 ggccgctgtt gctgaagaaa gcgtggacgg atcggcataa acggtgctta catcgtcaag
     6481 gtaatccgtg aacgtcgtgc gccacgtgaa atcccagcct attctccatt gtttattcat
     6541 cgtgaaataa aaccccatcg aagcgggaat gatgcctgta acggcggtgt agcgttttcc
     6601 ttccgtcatc agcgggcgca gggccacgta gtttccgttg taaagtgcct tcgggttgtt
     6661 gtacaccatg cctgctccga gtcctacata agcgcgaaaa ctgttcttgt gcttgaatgt
     6721 acgcccgagg tcgttgatct cgtaaaagca gaactgcgcc tgtgccgctg cttcaaaaag
     6781 atcgttgcgg aagctcaggt tccggccgtt cctgccggga ttggaggaaa gcctgtcatc
     6841 gcccgaaatc cgcgcccagc caatatcggc tttcacggaa aagaacgggt tgaccttgta
     6901 gcgcacgagg cctgtggtgg tcatgtgcgt ctgcgacatt ttcatatcgg aaacgaaatc
     6961 acgccgggtc agctgctttc cgcccatatc gccgaggtaa ttcgatgcgc cggttgaaat
     7021 gccgatgtcc cagtaatact gggcgtgcgc aatgaacgga agcaggaaaa gggaagacgc
     7081 gagaagcgcg gttttaacga agggagtagc ttttctcatg ttaaaccgtt ttggaggtta
     7141 gggtctttcg tgaacgttgt tttcttttac gaagggcggc ggcgtaaagt tgctgcctgt
     7201 gaataagttg tggatacaaa atataaaaaa aactgccgtc ccggaaggaa cggcagatgg
     7261 tttttggcgg caggcggatc agaacttcgc cctgactttc ctgtgcttgt actttttgcc
     7321 tttgaacatg tagctgtaat gcgatttgta gatggagctt ttgccgcgga taacgtagct
     7381 gaactcgacg cttgtcgtaa tgaatgagtc gttgtgcgtg ggatctccgc gcttgttcag
     7441 gtgaacgttg ccgtcgctgt cggtccaggt ctggtaaccg aaattgtttc cgaagccggg
     7501 gttgctgttc tgtgcgcccc atgcgtttgc tgccgcgtga ttggtacggt cggcgaccgc
     7561 cgctgcgccg ggaaccgtgt tctgaacgta aaacgtgctg gcgtcgtcga gataatcgga
     7621 gaatgtggtg cgccagtcga tgcaccagcc gatgcggtag tgcttgttga ccgtgaaata
     7681 gaaaccggca gtggcgggta ttgtcagcgt tactttcgtg taccgcacat tttcagtttc
     7741 caggggacgc agggcgatcc acgttccgtt gaaattgcct ttcgggttgt ggtaaagtcc
     7801 gccgatgccg agaccgatgt aggcgcggaa attattgcgg taacggtaag tgtgtccgag
     7861 gtcgttgatc tcgtagaaaa aatactggca ttccgcgctg agctcgaaca tctggttgcg
     7921 gaaattgaga tttctccagt aacgcggctc gttggtgcag aggctgtcgg cgccacggat
     7981 gcaggcgtag gaaaggttgc ctttgatgga gaactgcggt gatattttgt aacgcgcaaa
     8041 accgttgacc gcctggcgcg tttccttcat cttgatgtcc atcacaaaat cgcggcgcgt
     8101 cagctgcttt ccacccattt cgccgaggta attggatccg ccgacgccca cgccgaaatc
     8161 ccagaaatat tgcgcacgcg caatgaacgg ggtcagcaca ataactgcgg caaataaaag
     8221 tttcttcatg gttgaagttt ttacgcaaaa aaccgtgttt acgaagtaac aaaaaacagc
     8281 ccgcatttcc aaacggaaca gggctttatg tttactttta gcgcctgcct tttttatgaa
     8341 aaacaggatc acctcgcttt tcaatattga gtttcccgtc atacaggccg gcatgatctg
     8401 gtgcagcgga tgggagctgg cttccgcggt gagcaatgcc ggcggactcg gcctgatcgg
     8461 ctcgggttcc atgtacccgg acgtgctgcg cgggcatatc cgcaaatgca gggcggccac
     8521 tgcgaaacct ttcggcgtga atgtgccgct gctgtatccc gacattgaaa agcacatgga
     8581 cgtgatcatc agcgaaggcg tgaagatcgt tttcacgtcg gcgggaaacc cgaagacgtg
     8641 gacgcccctg ctgaagcaga acggaataac ggtggtacac gtggtgtcga acgtgaaatt
     8701 tgcggtgaaa tgccaggaag ccggggtgga tgccgtggtt gcggaaggct ttgaagccgg
     8761 cggccacaac ggacgggagg aaacgacgac catggtgctc attccgctgg tgcggaatgc
     8821 ggtttcgata ccgctcatcg ctgccggggg aattgcctcg ggccggcaaa tgctggccgc
     8881 gatggcgctc ggtgcggaag gcgtgcaaat cggttcgcga ttcgtggctt cggtggaatc
     8941 gtcggcgaat gagaatttca agcatgcggt gacggatgca aaggaaggcg acacgatagt
     9001 cacgatgaag cagctggtgc cggtgcgatt gataaaaaat aaattttaca aagcgataga
     9061 ggaggcggag ctgcgcgggg cgggtgcgga ggagctgaaa gcaattctcg ggcgggcgcg
     9121 tgcgaagaaa ggaatgtttg aaggcgatat ggaagaggga gagctggaga taggacaggt
     9181 gagcgcgatg gtgcgcgaaa taaaaccggc cgcggagatc atgaaggaaa tcgtggcgga
     9241 atacgaagcg gtgaaaaatg aattgtgtac aacggatcaa tcacctgcgt gagtgataga
     9301 agcggaaagc ccgcaatccg caggatgagg acttgcagcg gatagcacga ccccggtact
     9361 gcgcaaagca gaagtacggg ggcacgccca aaaaataaaa cataaagttt cacgtctcat
     9421 tagtcaatac gcaatgataa aattcgaccg attcaccctc accaacggac ttcgcgtgat
     9481 cgttcaccac gacgaagcga cgccgctgac ctgcgtcaat atcctgtatg acgtgggcgc
     9541 gcgcgacgag gaggaaagcc gcacgggatt tgcgcacctg ttcgaacacc tgatgttcgg
     9601 cggttccgtc aacatcccga attacgacga accgctgcag cgcgtgggcg gcgagaacaa
     9661 tgcgttcacc tcgaacgaca ttacgaacta ttacctgacg ctgccggcgg aaaacctgga
     9721 aacggctttc tggctggaat cggaccgcat gctgagcctc gcgttctcgg aaaaaagcct
     9781 ggaggtgcag cgcagcgtgg tgatcgagga attcaagcag cgctacctga accagcctta
     9841 cggcgatgtg tggctgctga tccgcccgct ggcgtacaaa gtgcacccgt acaagtgggc
     9901 gacgatcggg aaggagatcc gccacattga agaagcgaaa atggaggacg tgaaagcgtt
     9961 cttcgggaaa cattacaatc cttcgaacgc gatcatggtg gtgtcgggaa aagtgacgac
    10021 ggaaaatgtg aaagcgctgg ccgaaaaatg gttcggcccg ataccgccgg ggaaaaaacc
    10081 ggtacgggat cttccggccg aaccggagca gaatgaatac cgccgtgaaa cggtggtgaa
    10141 ggatgttccg ctcgatgcgc tgtacatgac gtttcacatg ggagaacgaa aggacagtga
    10201 gtattacgtg atggatatag tttccgacat tttatcgcgc gggaattcct cgcgcatgta
    10261 caaccggctg gtgaaggagg aacgcatttt tacggaagtg agcgcgttcg tgatgggcga
    10321 cctggacaag ggcctgttca tcgtgtcggg gaaaatttcg ccgggcgttc ccgtggaaag
    10381 agcagaagat gccgttttcg cggagctgca gaagctggtg gatgagaaaa ccggggagcg
    10441 ggaactgacg aaagtgaaga acaagatcga atcgtccatg cagttcggtg aaatgaacat
    10501 tctcgacaaa tcgatgaacc tcgctttctt cgaactgctc ggcgatgcgg cgatgatcga
    10561 tcgcgaagtg gaaaaataca acgcagtgac ggcggaagag gtgcagcgga ccgcggcgaa
    10621 aatactccgg aagaccaact gctcttcgct tttttataaa tcagcaagga aaaactgaac
    10681 catgagcatc gacagaacga cagcacctga atttcatacc atcggcagca ttgatattcc
    10741 ccgcgcggaa cgcgtgaagc tttccgcagg aaacgatctc tggctgctcg atgcgggcac
    10801 gcaggatgtg ctgcgcctcg actttatttt caacgcgggc tcgcgcttcc agcagaaaac
    10861 gctcgtcgca tccggcgtga acgagatgct cgacgaagga actgcgcagc gcagcgcgga
    10921 ggaaatagcg gaagaactgg attacttcgg cgccttcatc gagtcggaac cgggaaagga
    10981 tacgtcgtcc ttcaccgttt tcaccatgaa ccgccatctc gaaccggtga tgaagatcgt
    11041 gagcgaaatt ctttgcggcg cgtcgttccc ggaaaacgag ttccgcgtgt atatcgataa
    11101 caagaagcag aaattcatcg tcgattcgga caaggtttcc gttcttgcgc ggaggaaatt
    11161 cggtgagatt attttcggtg aaaaaaatcc gtacggcgct gcagtaaagc tcacggattt
    11221 cgatgccatc gggagaaaag acctcgacgc gttccacaag actttttacg caaaagagaa
    11281 ttgcacgatc gtggcgtctg gtaaaatccc gcagggactg gaagcgctgc tggaaaaata
    11341 cccggcggga aatccgggca aaaaagctgc agcggatgat ttcgcgccgt tctcgccttc
    11401 cgcggaaaga atacacaccg tcgaaaaagc cggcgcgatc cagtctgcca tacgcatggg
    11461 ccgcccgctg ttcaacaaaa cgcaccccga ttaccacgcg atgctcgtgc tgaataccgt
    11521 tctcggcggt tatttcggat cgaggctgat gtcgaatatc cgggaggaca aaggatatac
    11581 ttacggcatc ggctccggca tcatgtcgat gtacaatgcg ggctatttcg tgatctcgac
    11641 cgaggttggc gctgaagtga cgaatgcggc gctggaagaa atttattccg agatcgcgcg
    11701 cctgcagaat gatttgattc cggaagaaga actggaactt gtacgcaatt acatgaccgg
    11761 cgttttcctc cgcagcaccg acggaccttt tgcgctgggc gaccggctga aaggattgct
    11821 cgggtatggt ctcgggtacg attactacga gcgctatctc gaaacgatac gttcggtttc
    11881 ggcaaatgag ctgcgcgagc tggcgcagaa atacctccgg aaggaagata ttttcgagct
    11941 cgtcgccggc gtgagaaaat agtccattgc ggcgaaacga tctttctccg cgatcgtatg
    12001 aataatcaga tgaggacccc gttttacatt gcgctcattt tgctgctggc ttcgtttgcc
    12061 gcaggaaaaa cggaaaaaaa gtacgaccct gcgaagctgt tcacggtaaa gcagctgcag
    12121 gaagatttca cggcgctccg cgcacgcctg gaacggaaag atccgaacct ctatctctac
    12181 accccgaaaa aacgcctcga tttcgttttc gacagcctct acagccgcat cgaagcgccg
    12241 atgacggcca tggatttttt ccggctgctc gctgcgctcc gttccgagat ccgcgacggg
    12301 cacacttatc ccatggcaag ccgcgagatg gaaaacggtt ttttcaacaa ggattctgtt
    12361 ttcctgcctt atctttttgt ttacctacgt cataagcttt acgtgcgccg gagctttaca
    12421 ccggattcgg cgatt
//
LOCUS       DASTSF010000015         4630 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_428163,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000015 DASTSF010000000
VERSION     DASTSF010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4630
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_428163"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1813
                     /locus_tag="VFU15_00455"
     CDS             <1..1813
                     /locus_tag="VFU15_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional YncE family protein/alkaline
                     phosphatase family protein"
                     /protein_id="HEU4716268.1"
                     /translation="TCWGCDKVVLFDTEKRQLRGSVIVGDNPNDMALNTKGTLLFVSN
                     ANDNSVSVIDLQRNTVTETLNAALFPAAPPGSTTNSIALSEDDKTLFIANADNNCLAV
                     FDVSVPGHSKSKGFIPVGWYPTCVRVANRVIFVSNGKGFSSLPDPFGPNPYGNVNYQH
                     SDTTHHEEQYIGSLFQGTLSIIPVPDEKTLSVYSQQVYRNTPYTKEKEERADGQAGNP
                     IPTRTGDPSPIKYVFYIIKENRTYDQVLADMPGGNGDTSLLLFGKNITPNLHAIAGDF
                     VLFDNFYVDSEVSADGHNWSLGGYADDYLEKTWPTSYGGRGGSYDAEGNRDIANNKNG
                     FIWDFCKRFGVSYRTYGEFADDFKPNIPVLQNHFCPYYTSWDQSVMDTTRFSEWKRDF
                     DSLLSIGQVPQLNTLRFINDHTQGLRLGKPSPNAQVADNDLATGLFVDYLSHSPIWKE
                     SAVFILEDDAQNGPDHVDAHRSTLYVAGGFVKRKFIDHTMYSTTSVLRTMELILGLPP
                     MSQYDAAAEPLWRCFSDTANTAPFHARKAQANLFEKNVAMNQWQKMSEDFNFDKEDNV
                     NEDDFNQVLWWAVKGDSRPCPSPVRSAFIREDEDTDD"
     gene            1939..2862
                     /locus_tag="VFU15_00460"
     CDS             1939..2862
                     /locus_tag="VFU15_00460"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEU4716269.1"
                     /translation="MKKKKTLVVLLAATCCFFLVTGYNNGAGSHGWDCTGAETGLSNP
                     QGCKASGCHSSTATTGITLTVEFDSVGVPTTHYVGGMLYTVTITGTNTTAATLPKFGL
                     QVGCIRGSSAVTTPSNAGTWTAPFPAGTHHATPQSGNFVVDLVEHNTPNAATTGTGGT
                     GTTYVKTFNWTAPAAGTGTVSFWAALNAVNNNGSEDSGDKWNTNHIVIDEWGNAAGIA
                     GAAANAFSMNVFPNPVNGHASVSFTNASALTRGTIRVTDLSGKTVSETAVNDFGATTG
                     IDCTNLSNGVYFFSLIVNNEATVTKKIVIAN"
     gene            3080..>4630
                     /locus_tag="VFU15_00465"
     CDS             3080..>4630
                     /locus_tag="VFU15_00465"
                     /inference="COORDINATES: protein motif:HMM:NF027798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-1,3-glucanase family protein"
                     /protein_id="HEU4716270.1"
                     /translation="MFTTSLVIGKSALAFGNIHAIMYGQLLEAYTPSHGPQKGTALGK
                     DTWVYLSAGANPDYATAPSAGSSVEAFNVYDAPGTYSIGLPAVQIQSAHIVFGFGANP
                     SVISDATGAPQQPSPLTATCVYDFVEFTYNASNTLYLNTTMIDQFGMPIRIQVDPADA
                     VLPQGAGVIPQREFVFKSYSSFINSNPANAAYAQCMNDPFGNALTERILSPKYVIFGN
                     GVSGVSLSLLNQGKNPPVSTLPQGTYYYAVTALNANGKETFAQYLVSSITLPEANVAV
                     NIAWAPNDMQPAGTVSYNVYRGTPGGNSVSWVCVTNVPASNFPPGAGGAVADLGTTLN
                     SNAPTFNPLNTAFDAEITTFFTTYLSTAKSPTSLTLTASDGTSDGYVFTFTGYSHSDS
                     NGNITHLSMLLESVVDSAGGTVSAPPIPLQTAFNIYYPFWNTNTFNQNKPSPPSWTVY
                     PNDPASVMVLAAQGVFADNTAQPVPPGVTAANTVIYQTLLGNLENQVVAAITRGIANQ
                     PGNPQNWGN"
BASE COUNT         1110 a         1362 c         1188 g          970 t
ORIGIN      
        1 cacgtgctgg ggttgcgaca aggtcgtcct gttcgacacg gaaaaacgcc agctgcgtgg
       61 atcggtgatc gtcggtgaca acccgaacga catggcgctg aacacgaagg gaacgctgct
      121 ctttgtttcc aacgcgaatg acaattccgt ttcggtgatc gacctgcagc gcaacacagt
      181 gaccgaaacg ctgaacgcgg ctttgtttcc cgcagcgcct cccggttcca ccacgaacag
      241 cattgcgttg agcgaagacg acaaaacgct cttcatcgcc aatgcggata acaattgtct
      301 cgccgtgttc gacgtctccg ttcccggtca cagcaaaagt aaaggcttca ttcccgtggg
      361 ctggtacccg acctgcgtgc gcgtggcgaa ccgggtgatc ttcgtttcca acgggaaagg
      421 attttcttcg ctgccggatc cgttcggacc gaatccatac ggaaacgtga attaccagca
      481 cagcgatacg acgcatcacg aggaacagta catcggctcg ctgttccaag gaacgctcag
      541 catcattcct gttcccgatg aaaaaacatt gtccgtttat tcgcagcagg tttaccgcaa
      601 cacaccttac acgaaagaaa aagaagagcg ggcagacggg caggcgggta atcccattcc
      661 caccaggacc ggcgatcctt cgccgatcaa atatgttttt tacatcatca aggaaaaccg
      721 gacgtacgac caggtgctcg ccgatatgcc gggcggcaac ggcgacacat ccctcctgct
      781 cttcggaaaa aacattacgc ccaacctgca tgcgatcgcc ggggattttg tgctgttcga
      841 caatttttac gtggacagcg aagtgagcgc ggacggtcac aactggagcc tgggcggcta
      901 cgccgacgac tatctcgaaa aaacgtggcc caccagctac ggcggaagag gcggctcgta
      961 cgatgcggaa ggcaaccgcg atatcgccaa caacaagaac ggcttcatct gggatttctg
     1021 caagcgcttc ggcgtgagct accgtacgta cggagaattt gcggatgatt tcaaaccgaa
     1081 tatccccgtg ctgcagaacc acttctgccc gtattacacc agctgggacc agtcggtaat
     1141 ggacaccacg cgtttttcgg aatggaaacg ggatttcgat tccctgctca gcatcgggca
     1201 ggttccgcag ctgaacacat tacgtttcat caacgatcat acgcagggcc tcaggctcgg
     1261 aaagccttcc ccgaacgcgc aggtcgccga caacgatctt gccaccggcc tgttcgtgga
     1321 ttacctcagc cattccccga tctggaagga aagcgctgtt ttcattctcg aagacgacgc
     1381 gcagaatggc ccggaccacg tggacgcaca ccgcagcaca ttgtatgtcg ccggcggttt
     1441 cgtgaaacgc aaattcatcg atcacaccat gtattcgacc acgtcggtgc tgaggacgat
     1501 ggagttgatc ctcgggcttc cgccgatgag ccagtacgat gccgcggcgg aaccgctgtg
     1561 gagatgcttc agcgatacag ccaatacggc gccgttccat gcacggaagg cgcaggccaa
     1621 cctgttcgaa aagaacgtag cgatgaacca atggcagaag atgagcgaag atttcaattt
     1681 cgacaaagaa gacaacgtga acgaggatga tttcaaccag gtgctttggt gggccgtcaa
     1741 aggcgacagc cgtccctgcc cttcccccgt gcggtccgcc ttcatccgcg aggacgagga
     1801 caccgacgac tgacatgttt tgtcaccccc cttttcccgt atgaaataat agtgaatgcc
     1861 ggcatttttt tagttgtatt aacacctatt tttgggctgc tcaaacaaaa cattctatca
     1921 caccagaaca aaaacttcat gaaaaaaaag aaaactctcg ttgtattgct tgctgcaacc
     1981 tgctgctttt tccttgtcac aggttataat aacggagcgg gaagccacgg atgggattgc
     2041 accggcgctg aaaccgggct gagcaatccg cagggatgca aagccagcgg ctgtcattcc
     2101 tctactgcca ccaccggtat tacactgacg gtcgaattcg attcagtggg cgtgccaacc
     2161 acgcattacg ttggcggaat gctgtacacc gtcacaatca cgggaacaaa cacgaccgcg
     2221 gcgacgctgc cgaaattcgg gttacaggtg ggctgcatcc gcggctcttc ggccgtgacc
     2281 acaccaagca atgcgggaac atggacagct ccgttcccgg cgggtacgca tcatgccaca
     2341 ccgcagtcgg gaaattttgt agttgatctc gtggagcaca atacgccgaa cgcggcgaca
     2401 acaggtacgg gcggcaccgg cacgacgtat gtaaagacgt tcaactggac cgcacctgcg
     2461 gccgggaccg ggaccgtttc tttctgggcc gcgctgaatg ccgtgaacaa caacggcagc
     2521 gaagattccg gtgataagtg gaacacgaac cacatcgtga tcgacgaatg gggcaatgct
     2581 gccggcatcg ctggtgcagc tgcaaacgct ttcagcatga acgtattccc gaacccggtg
     2641 aacggccatg cttccgtttc attcaccaat gcaagcgcgc tgacgagagg cacgatccgc
     2701 gtaacggacc tgtccggcaa aacagtcagc gaaaccgcgg taaatgattt cggtgcaacc
     2761 accggcatcg attgcacgaa cctcagcaac ggcgtttact ttttctcgct catcgtgaac
     2821 aacgaagcga cggtcacgaa aaagatcgtg atcgcgaact gatttatctt acaacttttc
     2881 ttgtgaaagc cttccggaaa cggggggctt tttctttccc gcttcccgga tgttaacgca
     2941 ggcgtttcgt cctgtttacc ggcgcaatca aaaaaatttg tttatttatc aatattccat
     3001 gaacccaacc ggctgaacgg aaaacaagtt catccatcca cctgaaatct tgcatcattc
     3061 atattaaaaa accgcatcta tgtttacaac atctctcgtc atcgggaaaa gcgccctggc
     3121 attcggaaac attcacgcca tcatgtacgg ccagctgctc gaggcttata caccttcgca
     3181 tggtccgcag aaaggaaccg cgcttggaaa agatacatgg gtctacctct ctgccggagc
     3241 gaatcccgat tacgccacag cgccttccgc aggcagttcc gttgaagcct tcaacgtgta
     3301 tgatgcaccc ggaacgtatt ccatcggtct tcctgccgtg cagatccaat cggcgcacat
     3361 cgttttcggt ttcggcgcca atccttccgt gatcagcgat gccacgggag cgccgcagca
     3421 gccgagcccg ctcacggcca cctgtgttta tgacttcgtg gagtttacct acaatgcttc
     3481 aaacacgtta tatttaaata ccaccatgat cgaccagttc ggcatgccga tccgcatcca
     3541 ggtcgatcct gcagacgctg ttcttccgca gggcgccggc gttattcccc agcgtgaatt
     3601 cgtattcaaa agctacagct cgttcatcaa cagcaatcct gccaacgccg cttacgcgca
     3661 gtgcatgaac gatccgttcg ggaatgcgct cacggaacgc atcctttctc ccaaatacgt
     3721 catcttcggg aacggcgttt ccggggtttc tttgtcgctg ctcaaccagg gaaaaaatcc
     3781 gccggtatcg acgcttccgc agggcactta ctattatgcc gtcaccgcgt tgaatgcgaa
     3841 cggcaaagaa accttcgcgc aataccttgt ttcttccatc acgctgccgg aggcgaacgt
     3901 tgctgtaaat atcgcgtggg cgccgaacga catgcagcct gccggaacag tgagctataa
     3961 tgtataccgc ggaacacccg gcggcaattc ggtaagctgg gtctgcgtca cgaacgttcc
     4021 ggcttcgaat tttcccccgg gcgcaggcgg ggcggtggcc gacctgggaa caaccctgaa
     4081 ttcaaatgcg ccaacgttca atccgctgaa tacggcattc gatgcggaga tcaccacgtt
     4141 cttcacgacg tatctcagca ctgcaaagtc acccacgtcg ctcacgctta ctgcatccga
     4201 cggaacatcc gacgggtacg tgttcacttt caccggctat tctcattccg acagcaacgg
     4261 gaacatcacg catctttcca tgctcctcga atccgttgtc gattcagccg gggggacggt
     4321 aagcgccccg ccgataccgc tgcagacggc cttcaacatt tattacccgt tctggaatac
     4381 gaatacgttc aaccagaaca aaccttcacc gccttcatgg accgtttacc cgaacgatcc
     4441 ggcttccgtc atggtgctcg cagcacaggg cgtttttgcg gacaataccg cgcagccggt
     4501 tccgccgggc gtaacggcgg cgaataccgt gatctaccag acgctgctcg gcaacctcga
     4561 gaaccaggtc gtcgcggcca tcacgagggg catcgccaac cagccgggca acccgcagaa
     4621 ctggggcaac
//
LOCUS       DASTSF010000016         3118 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_205502,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000016 DASTSF010000000
VERSION     DASTSF010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3118
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205502"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..236)
                     /locus_tag="VFU15_00470"
     CDS             complement(<1..236)
                     /locus_tag="VFU15_00470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716271.1"
                     /translation="MHEFIIVSRNKGNSCFPVNKDLKMGHTPRRVSDLLFERCKEKPD
                     SRSLRLHWQIEPLSVTLLFPRTGNSPTIMQEVKP"
     gene            236..811
                     /locus_tag="VFU15_00475"
     CDS             236..811
                     /locus_tag="VFU15_00475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716272.1"
                     /translation="MNFPKSFFLPIILFFFACNAFGQDILYLQKKDHPHRFRKLPLDR
                     EYRLKTKDSLYFSDIFSVTDSTLSVIAPVFRSDSETVYDMNGKPSGKKAITRLAGNDT
                     VIIPFSRIQYIRKDFVKNNQWVVPFIYGGFGAAAGILFLPFALIDGPDTFHNWILFEG
                     VLVGVCGPALLIGVGSRKFDTQEKWELKRTK"
     gene            1071..2672
                     /locus_tag="VFU15_00480"
     CDS             1071..2672
                     /locus_tag="VFU15_00480"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEU4716273.1"
                     /translation="MKKLFTLAFLLPVAATMFAQPANVNVSNTTYFEGEPYVAVNPTN
                     HNNVVIAWMALDVNTLFRVGIKTKVSFDGGLTWGNMHVQPHFGSGWGSADVSMQFRSN
                     GTLYLSYIDYKASADSGGVFITHSLDGGITWSAPTQVWNANTEDPSKLPLDRPWLAAD
                     NSGTANDGMFYITTKPAPWIPAPNRPYLKVSSDSGQTWSAYRYIDTTNYLVGNIIQAP
                     MATLTTSADGALCIAYPSYLASQSVYPKIFFAKSYNHGNSFTYHDLLVGGTPVTDTLY
                     KLGYRLAANPSNPNQLAYAFMEQPYGDPDIFICTTNDGGNTWSAPLRVNDDAQGNGKA
                     QDMVWADYDTNGKLVVTWRDRRNGSGTGFYQSSDTYCAVSTDNGATFMPNMRLSSMTA
                     PFDSILEQKGNDFLSCRLLNDTIYAAWGDVRTGNLNIFFAKTSDSTGITSTMTTVDGE
                     ENDISVYPNPSSDKIVLSFGDEMKQPVTVKIFSGNGQQVMAQSFAGGTLKATMDISQL
                     GAGVYFISIESGKNILQTKKLVITR"
BASE COUNT          788 a          867 c          762 g          701 t
ORIGIN      
        1 tatggtttta cttcctgcat aatcgttggt gaattcccgg ttcgggggaa taacaaagtt
       61 acgcttaaag gctcaatttg ccaatgcaga cgcaggctcc ggctatccgg tttttctttg
      121 catcgttcaa acagcagatc agaaacccgg cgtggagtgt gacccatttt caaatctttg
      181 ttaaccggga aacaggaatt gcccttattc cggctaacga tgataaattc gtgcaatgaa
      241 ttttcccaaa tccttctttc ttccgattat tttattcttt ttcgcgtgta acgcttttgg
      301 ccaggatatc ctttacctgc aaaagaaaga tcatccgcac cgcttccgga aactgccgct
      361 ggaccgcgag taccggctga agacgaaaga ctccctttat ttttcggata tcttctccgt
      421 taccgacagc acattgtcgg tcattgcacc cgtattccgc agcgacagtg aaaccgtata
      481 cgacatgaac gggaaacctt ccggcaaaaa agcaattacc cgtctggcag ggaacgacac
      541 cgtgatcatt cccttcagcc gcatacaata catccggaag gatttcgtca aaaacaacca
      601 gtgggttgtt ccgttcatat acggcggttt cggtgcggcg gcagggatcc tgttccttcc
      661 gttcgcgctg atagacggtc ccgacacgtt ccacaactgg atacttttcg aaggcgtgct
      721 cgtcggcgta tgcggacctg cattgctcat cggtgtggga tcacggaaat tcgatacgca
      781 ggagaagtgg gagctgaaga gaacgaaatg acgtacacaa ccgggattcc ctggtttttt
      841 cctgcgtgat tttgtgtacc ggtgagaata tcattgcctt atcccgtcac aaaggcgcgg
      901 aggcgcagaa ctcttttgca atcccaaaca ggaaagctta ttaaaccttt ttcttttctc
      961 ttcatctaat attgttatta aaaaacgatt catgcctttt acctaaattt aagctaccgc
     1021 acaaaccgaa aatccgacaa ctcaaaaccc taaaccaccg attgctgctc atgaaaaaac
     1081 tttttacgct tgcatttctc ctgccggttg ccgcaacaat gttcgcgcaa ccggcgaacg
     1141 tcaacgtgtc gaacaccacg tatttcgaag gggaacccta cgtcgcggtg aatcccacga
     1201 accacaacaa cgtcgtcatc gcgtggatgg cgctcgacgt gaacacgctg ttccgtgtgg
     1261 gcatcaaaac gaaagtgagc ttcgacggcg gactcacctg gggcaacatg cacgtgcagc
     1321 cgcatttcgg cagcggctgg ggctctgccg acgtatcgat gcagttccgc tccaacggaa
     1381 cgttgtattt aagctacatc gactacaaag catccgccga ttccggcggc gttttcatca
     1441 cgcattcgct cgacggcggc atcacgtggt cggcgcccac gcaggtatgg aacgcgaaca
     1501 cagaagaccc ttccaagctt ccgctcgacc gcccctggct ggcggcggat aattccggca
     1561 cggcaaacga cggcatgttc tacattacga ccaagccggc gccatggatc cccgcaccga
     1621 accgtccgta cctgaaagtt tcatccgaca gcggccagac atggagcgct taccgttaca
     1681 ttgacacgac gaattatctc gtcgggaata ttatccaggc cccgatggct acgctcacca
     1741 catccgccga cggagcgctg tgcattgcgt acccgagtta tctcgcttcg caatctgttt
     1801 atccgaaaat attcttcgcg aaatcataca accacggcaa cagtttcact tatcatgatc
     1861 tgcttgtggg cggaacgccc gtaaccgata cgctttacaa actcggttat cgtcttgccg
     1921 cgaatccttc caacccgaac cagctcgcgt acgcattcat ggaacagccc tacggcgatc
     1981 ccgacatttt catctgcacc acgaacgacg gaggcaacac ctggagcgca ccactgcgcg
     2041 tgaacgatga tgcacagggc aacggcaaag cgcaggacat ggtgtgggcg gattacgaca
     2101 cgaacggaaa actcgtcgtc acctggcgcg accgccgcaa cgggagcgga acgggatttt
     2161 accagtcgag cgacacgtac tgcgccgtga gcaccgacaa cggcgcgacg ttcatgccga
     2221 acatgcgcct gagcagcatg actgcgcctt tcgattccat tctcgagcag aaaggaaatg
     2281 attttctttc ctgccgcctg ctgaacgaca cgatctatgc agcgtggggc gacgtgcgca
     2341 cgggcaacct caatattttc ttcgcgaaaa cgtccgactc caccggcatc acgtcaacaa
     2401 tgacgacggt ggacggagag gaaaatgaca tttccgttta tccgaacccg tcgagcgaca
     2461 agatcgttct ttccttcggc gatgaaatga aacagccggt gacggtaaaa attttcagcg
     2521 gaaacgggca gcaggtgatg gcgcagtcct tcgccggcgg aacgctgaag gcaacgatgg
     2581 atatttcgca gctcggcgcg ggagtttatt tcatttcaat tgaatcgggg aaaaatatcc
     2641 tgcagacgaa gaagctcgtg atcacgcggt gatgcaaatc ccgggcaaca aacgaaaact
     2701 gtttaaccac gaagtgtccg aaggtctttg caaagcgaat gaaggccgac aataaactgg
     2761 atcagttctc gacttcgctc gaactgacag acttcgcccg aactggcaaa gttttttccc
     2821 ggtgtcttcg tgagagtcgt ttttaacacg gaggttcact gaggttggtt cacagaggtc
     2881 cgcggggaac agctaacgat taactgaaaa cagaaaaaac taaaccaaaa acgcctcccg
     2941 gtatgataaa acaaacactg ctccctgctg cgttcttcct ttcggtcatg acaacaaccg
     3001 cgcaatccac caccttccag gaggcataca gccagcccgg aacggatttc ggcagcaaag
     3061 tgatttcatc cgcatccggc tacatgatcg gcggaacgag ctccgcttcc ggggatga
//
LOCUS       DASTSF010000017         2760 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1767732,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000017 DASTSF010000000
VERSION     DASTSF010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2760
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1767732"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1540
                     /locus_tag="VFU15_00485"
     CDS             <1..1540
                     /locus_tag="VFU15_00485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716274.1"
                     /translation="HFRNVYSTLFHGIEPYHYFEMWLAAFFMDLSGGMISHLQCLRMC
                     AYGFILAMCAAGMIAICETIHGKVKLLHVAVSLLFLFFIPNIAQLFFNYDHHFSYPLE
                     CNPVNRINFRTYWMFLMPSFIFFLRKEYLAGVIALLLLPVISITTAPAVFGTIALLVV
                     VNARSRFFDRKQLWLICGAVLVMVLCFGLLFSLFKIKGIKALYSFSPAYILAYFKGSW
                     KAVLYTVFMEAVNVLVVASPFILVLLLLNRGKENLKRFAGGNRFILSFLFLALVAGVL
                     FFQATSFMNNAYQFVFIAYCVLALTIFILIQKTCADILSASAGTTVQLLLILFLVFSG
                     IRLAGISRHSLFSGQELYKKEFKPYSRDYLSQVSERMKLIGKAPGGYIGDSSYYAELY
                     YSKRLPDFYFPGLSYFIFAGTDDSYQFCLSDTAAITFGYRGTPRDLNFLRQAVASSLF
                     QMDFYSAPSPLSIGEKRRQAIKKYNIAYMILCPGVDPDPEWAGMAREKITDKVTGETF
                     VTLR"
     gene            complement(1592..2707)
                     /locus_tag="VFU15_00490"
     CDS             complement(1592..2707)
                     /locus_tag="VFU15_00490"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEU4716275.1"
                     /translation="MLHSTSLNKKERHRLRELDFLRGIAIILVLFRHRFLFLFSYRMG
                     WIGVDLFFVLSGFLVSGLIFRENKLTGSFRPGTFLVRRGFKIYPLYYFSLAAYSTVQY
                     LRHDFAFRSLKGDAFFIQNYLNGWGMIFPASWSLAVEEHFYFGIALLFALSAVRKGVA
                     SAGRYGVEIVIGAVMLLCLALRIYYACAFPGQIARGFTMTHLRIDSLAAGVLISYWFH
                     YKKEKLIRIAGKLKYLLPPAGIALLLFTQFLDPFKSLFVRTGGFTMLWCGFGMILIFF
                     ITETDINKKLNRIFSRPVVSLTSRIGVYSYAIYLIHAMVNDFVFKYFHGKSYFGFALY
                     FFASILAGALMTELIEKFFLKIRDRYFPGSFQPLAAS"
BASE COUNT          665 a          677 c          677 g          741 t
ORIGIN      
        1 gcatttccgc aatgtgtaca gcacgctctt tcacgggata gagccgtatc actatttcga
       61 aatgtggctc gccgctttct tcatggattt gtcaggcggg atgatcagcc atctccagtg
      121 cctgcgcatg tgcgcctacg ggtttatcct ggcgatgtgc gctgcaggaa tgatcgcgat
      181 ctgcgaaacc atccatggaa aggtgaagct gctgcatgtt gcggtttctc ttctctttct
      241 ttttttcata ccgaacatcg cacagctttt ttttaattac gatcaccatt tcagctatcc
      301 gctggagtgc aatccggtga accgcatcaa tttccggact tactggatgt tcctcatgcc
      361 gtcattcatt ttctttctgc ggaaagaata cctggcgggt gtgatcgcgc tgctcctgct
      421 cccggtcatt tcaataacca ccgctcctgc agttttcggg acgatcgcgc ttctcgtcgt
      481 ggtgaacgcc cggagccggt tcttcgaccg gaaacagcta tggctgatct gcggtgctgt
      541 tctcgttatg gttctttgtt tcgggctgct gttttcgctt ttcaaaataa agggcataaa
      601 agcgctttat tctttttccc ccgcttatat cctggcttat ttcaaaggtt cctggaaagc
      661 ggtgctctat acggttttca tggaagcggt gaatgtactc gtggtcgctt cgccgttcat
      721 cctcgttctt cttctgctta accgcgggaa agaaaacctg aagcgttttg ccggcgggaa
      781 ccggtttatt ctttcctttt tgttcctggc gctcgtggcg ggcgttcttt ttttccaggc
      841 gacgagcttt atgaacaatg cttaccagtt cgttttcatc gcctattgcg tgcttgcact
      901 cacgatcttc attcttattc agaagacctg cgcggatatc ctttccgctt ccgccggaac
      961 gacggtgcag ttgctgctga tcctttttct tgttttttcc ggaattcgcc tggcaggcat
     1021 aagcaggcat tcgcttttct ccgggcagga actttataaa aaagaattca aaccttattc
     1081 acgcgattac ctttcgcagg tgagtgaacg catgaagctg atcggtaaag ccccgggcgg
     1141 ttatatcggc gattcatcgt actatgcgga actgtattac tccaagcggc tcccggattt
     1201 ttatttcccg ggcttgtcct atttcatttt cgcgggaaca gatgattcct accagttctg
     1261 tctttccgac actgccgcta tcactttcgg ttaccggggc acgccgcgcg atctgaattt
     1321 ccttcggcag gctgttgctt catctttgtt ccagatggat ttttattcgg cgccatcgcc
     1381 gctttccatt ggggagaaaa ggagacaggc cataaaaaaa tacaacatcg cctatatgat
     1441 cctgtgcccc ggggtggatc ctgacccgga atgggcaggc atggccaggg agaaaataac
     1501 ggataaggtt accggtgaaa cgttcgttac gctcaggtga atgcgctgat cgccggaaac
     1561 ggtttgcttt ttttgtaacg gatttgaaat ttcaggatgc tgccaggggt tgaaaagaac
     1621 ccgggaaata acggtcgcgg attttcagga aaaatttctc aatgagttcc gtcatcagcg
     1681 ccccggcgag gatgctggcg aaaaaataca gtgcgaagcc gaaataactt ttgccgtgaa
     1741 aatatttgaa aacaaaatca ttgaccatgg catggataag atatatggcg taggaataaa
     1801 ccccgatgcg gcttgtcagt gaaacaacgg gcctgctgaa aatgcgattc agctttttgt
     1861 taatgtccgt ttccgtaatg aaaaaaatga ggatcatgcc gaagccgcac cacagcattg
     1921 taaagccgcc ggtcctgaca aaaagcgatt tgaacggatc gagaaactgg gtgaacagca
     1981 ataatgcgat tcccgcagga ggcagcaggt acttcagttt tcctgcgata cggatcaatt
     2041 tttctttctt gtaatgaaac cagtagctga tgagtacgcc ggccgccagt gaatcaatgc
     2101 gcaggtgtgt catcgtgaat ccccgggcga tctggccggg aaaagcacat gcataataga
     2161 tcctcagtgc aagacataaa agcatgacgg cgccgatcac aatttctacc ccgtacctgc
     2221 ccgctgacgc gactcctttg cgtacggcgc ttaacgcgaa cagtagcgca atgccgaaat
     2281 agaaatgttc ttccacggcc agcgaccagc tggcgggaaa tatcattccc catccgttca
     2341 ggtaattctg tataaaaaaa gcgtcgcctt taagggaacg gaacgcgaaa tcatgcctga
     2401 ggtattgaac cgttgaatag gcggccaggg aaaaataata gagaggatat attttaaatc
     2461 cgcggcgcac caggaaagtc cccggcctga aacttccggt aagcttattt tcccggaaga
     2521 tgagtcctga tacgagaaag ccgctcaaca cgaaaaacag gtccaccccg atccagccca
     2581 tccggtacga aaagaggaaa agaaaacggt gcctgaacaa aacgagaatg atggcgattc
     2641 cccggagaaa atccagctcc cttaagcggt gtctctcttt tttattcaga gaggttgaat
     2701 gtagcataaa agcagtttac cgaatataat gaaccgttac agttttctga aacgcatccg
//
LOCUS       DASTSF010000018        13982 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1545829,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000018 DASTSF010000000
VERSION     DASTSF010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 13982)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13982)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13982
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1545829"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1306)
                     /locus_tag="VFU15_00495"
     CDS             complement(<1..1306)
                     /locus_tag="VFU15_00495"
                     /inference="COORDINATES: protein motif:HMM:NF022481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HEU4716276.1"
                     /translation="MTARYKLLNNVLGWIIFLVAAIVYISCIEPTASFWDCGEYIACC
                     NKLEVGHPPGAPTFLIIGRFFSLFAKDPAHIAACVNTMSALASAGTILFMFWSMTLLG
                     KRLVKAMKQELTDSRMYAIFGSAVIGSLAYAFSDSFWFSAVEGEVYGMSSFFTALVFW
                     AILRWDADDSERADRWLVLIAYMIGLSIGVHLLNLLTIPSIVFIYYFKKHQPTRSGLL
                     ITSLVAVGLLGVVQNGIIPGVVSTAANYELLFVNGFSLPFNSGTIIYFALLLGMIITG
                     LQYSMKGGRNLFRNFVVVASTFFLFTVIGAFAYSKVLMSVLTAAGAGYLIYKIRDNRV
                     LLNTILVSFSVFLMGYSTFLILVIRSKANTPMDENNPENAISLLSYLNREQYGDWPIA
                     YGPYYNAPLDPDDPADDGTPVYAQDIRSGDYAIIDARKQTNYN"
     gene            1455..1527
                     /locus_tag="VFU15_00500"
     tRNA            1455..1527
                     /locus_tag="VFU15_00500"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1488..1490,aa:Gln,seq:ttg)
     gene            complement(1616..2284)
                     /locus_tag="VFU15_00505"
     CDS             complement(1616..2284)
                     /locus_tag="VFU15_00505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716277.1"
                     /translation="MLFAHHRESYYQVTFPDTEFVDHLKFYAHAQKSNRDYYRAARLY
                     RIDEVPADGRTVMQQTELSSTILSSFNSNEINLNSARVKKLLPVVKNEDDQPLVIDSV
                     AGFQVKHYIVAELEKGNSYAIYFGDERMGFPAYDLRYFREKVPRQPPVIRAGKRNDTA
                     KEKVSAGVLADTTPGSSRVKAKAIASSSATPWYENKLFTWIAIGTVILLLGIMTVRML
                     KEMG"
     gene            2516..2836
                     /locus_tag="VFU15_00510"
     CDS             2516..2836
                     /locus_tag="VFU15_00510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716278.1"
                     /translation="MIIVFGIRKFSIYTFSPSELGFTDPSWNGIQFKVLQQYFHLMWI
                     PFFPVGKKWAISKPDGKLYEVPHNILGHLGDPSAKVRTPWYAFAGLILAGVAGIISGI
                     SNMM"
     gene            2938..5622
                     /locus_tag="VFU15_00515"
     CDS             2938..5622
                     /locus_tag="VFU15_00515"
                     /inference="COORDINATES: protein motif:HMM:NF024893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HEU4716279.1"
                     /translation="MSENAEIPAVPEVRRPLWKKILRFTLWTGGIFVFLILTLSVLTW
                     IFRDDIKKYVFAEACSRVKPEIVVQPSDIDVYIISSFPDVTVQLNNVAAIDTAHHVKN
                     DTLLKAGRIELAFNMLDLFHHNYTIRNITIENGALNLWDDAKGNDNYHFLKEDTAKTT
                     DTSHVDFALEKVQLKNMACSYRDKKSGDYYSVTFTDIFFKGKFTEKNFGFSSDADFLV
                     NKLLYGKNSFFEGDRGNFSVDMDIDRTTNTYSIRKTNIAIEKLSLGVTGSVTETGKKY
                     ATDLTVKGEQVELTSVLSLLPVSYHKNIADFESTGEFFVDGTVKGIYSDSSLPVIDSK
                     FGIRNGGTLKRRSGKGELHDISLNGTFTNQKGKDGLVISSFKASGAKSIFSGSFSVLG
                     FAQPKYDAKLSGRIDLGELYDILQPDTLESASGTVNISLEGSGKPSKAGKFTIADFRK
                     FRTQGNMGLANVAVKMKGAKSPVDSINGNLSFDGNNVGISHFTARSEKSDIVIDGTVR
                     NLLGFLFTKKEVLDVSGKFSSNNLDLNAILAENSGETGTTDTTYHLVLPERLKLNLDA
                     AVKKIAFRLFEAENVAGKVSLSGQRLIADPLSLNTMGGSISGSGMIDGTMEDSLLMTC
                     NANISQVDITKLFYEMENFGQGPDDEVITDKNVKGRLTSTVNFASLWGNDLSVNEKKI
                     FTDADVTIEQGELIDFKPLSVLSRFIKLDDLMDVRFKNLHNTITIRNRVISMPKMEIS
                     SSAMNVTMSGTHDFDNNVDYHFIVDLDELRANKVKKSKPQNSDFGQEIDDGGHRYRLF
                     IIMKGPIDDPDVKYDSKGFFQQKKEELKQEKQNLRQLLHDEWGIFRDDSAGSTDPKKK
                     GDRKTDDGAGKFILQQSDKPAKTKKKPKDQDLDDSGDY"
     gene            5716..7293
                     /locus_tag="VFU15_00520"
     CDS             5716..7293
                     /locus_tag="VFU15_00520"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEU4716280.1"
                     /translation="MRSLVLFITALTLSFSSPAQVFEMTYGTSGSEDAYPMEALPDGG
                     FILAGSSWGNGAGGKDVMLVRTDSLGNLLWSKQYGGSGDETALYIIRLSDGGFAVCGE
                     SFSDDVNGDAFLFRTDSLGNLSWWKNYGAANYDIAYSVVELHDGNLAISGLTETTPSQ
                     YDAFVAETDATGDTLWTQTIGGPGIDHAVQMIETSDDAILFCGKSLSYGAGSCDCFLV
                     KIDENGDTLWTKTIGGAGWDESMDVIERPDGFAICGGTTSSGSGSYDFLLMKTDLSGN
                     LQWAKTYGGAKIEASYCIIDVAGTGYAFAGYTETFGPGHSRGTDSANAMLILTDYSGD
                     TIWAASYGGLLKEECFSVRQTHSGGYAIAGYTGSFGDSLQAYLFATDPQGNTGCNDRR
                     AHPDVANANFACTSYAYDVFHSLHENSFAAAEAPLYFSPRQVCGGPLSSGNRNETGPL
                     LLYPNPAAGFVTVCTDEKETTQITLKDVTGKTAGTYFTGANGNVVINTNGLAAGIYFV
                     SFVKKDGSCISRKFIIQ"
     gene            7534..8508
                     /locus_tag="VFU15_00525"
     CDS             7534..8508
                     /locus_tag="VFU15_00525"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEU4716281.1"
                     /translation="MKKIYLFPALAGAVMCVTANAQTSTLFTENFNGNTYGFTLNTTD
                     ASSTATGANTWVVNNSYTGGSGTATCQSFSVPFTINNTAQEPAGITGQPTSKYMHILS
                     DSAQMQGITCGCYMAADGFCTMAESMFSKMSSDISTTGYSGVSFNFWWLCGGSANGYG
                     LVYYSTNGGTTWTALSTQYNNSSSAWTQASVTNAAFDNQATLRFGFQFVNNTTFNAAD
                     PGFCIDDVTITGSTATGMAENNLVSSVNLFPNPANDHITLTWNGAVPADNKMLISIYD
                     VTGQLVKTETRDFAVRSEMDDLALANGIYTFRVQCGEQTAISKITVAE"
     gene            complement(8582..10003)
                     /gene="asnS"
                     /locus_tag="VFU15_00530"
     CDS             complement(8582..10003)
                     /gene="asnS"
                     /locus_tag="VFU15_00530"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011586526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HEU4716282.1"
                     /translation="MAMEKIRDLLLSTELDRDVQVSGWVRSARGNKYVQFIHINDGST
                     IKNIQAVADVAKFEEALLKRVTTGACISVTGKLVASQGKGQSSEIQAATIEILGDCDP
                     ETYPLQKRGKDEKDKKTQTLPLEFLREKAHLRPRTNTFGAVLRIRHHMTFAIHKFFND
                     RGFFNIHTPIITGSDAEGAGEMFRVTTLDLDHLPRTKEGKVNFEEDFFGRETNLTVSG
                     QLEGETAALALGLIYTFGPTFRAENSNTPRHLAEFWMIEPEMAFYDINDNMNLAEEML
                     KYLVSYALHHCKDDIQFLNDTYDNQLLERLQSVIEHPFARISYTEAVDILKEAMGTKK
                     FEFTPEWGHDLQKEHENYLVEHFSGPVIVTGYPKAIKSFYMKQNDDGNTVRAMDILFP
                     WIGEIVGGSQREDDHDKLYARVKELNIPEESLWWYLETRKFGTCPHSGFGLGFERLLL
                     FVTGMTNIRDVIPFPRTPKNAEF"
     gene            10297..10911
                     /locus_tag="VFU15_00535"
     CDS             10297..10911
                     /locus_tag="VFU15_00535"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEU4716283.1"
                     /translation="METKERIVSAAFELFFRYGIKSVTMDDIAKHLSMSKKTIYQFFR
                     DKDEVVHTLMERALEEDHRDFENISKNSENVVESVFVMMKKMHDIFSNINPNIFYDLR
                     KYHPKTWDLFHRFKTEFVMGMVESELEKGKKEGLVRADINSRVLSRLRGEEIEMGFDP
                     SLFPPDKFRILDVQLALTEHFLYGICTLKGHKLINKYKQIVEEE"
     gene            10972..12333
                     /locus_tag="VFU15_00540"
     CDS             10972..12333
                     /locus_tag="VFU15_00540"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEU4716284.1"
                     /translation="MKYARITLTWIAAAAIPALLSAQQTDTSSYAFSLQQSVDFALQH
                     NTAYQNVLLDETAATAKVKEVTGIGLPQVNGSFQVQDFLEIPTSMIPGEFFGGPPGSY
                     IPVKFGTKYNATAGVTASQLVFSGPWLVGLQAARLYQDLALKNADRTKIETSCDVTKA
                     YYNALVTTKKKELLDANLDRVKKVMDDTKAFYDNGFVEKIEVDRITVTYNNLVTEVSN
                     AQRLMDLSMVMLKYQMGMDQSAKLTLTDNIENISFTPAETQNGKFDYTKRVEFQLMDM
                     QLRGKELAFRADRMGYFPTVALFGSAQAQAQRTKFDFLDGTSQRWFPVVIVGLQVSVP
                     IFDGFQRHYMIQQDRVGILQAQNDLLFMQRTIDMQQAVAKTTLTNSAATLAQQKANLD
                     LAQEVFDAAKTKYDQGVGSNLEIINAQTALKEAQTNYFDALTSAVIAKVDYDKATGTF
                     NVK"
     gene            12368..13504
                     /locus_tag="VFU15_00545"
     CDS             12368..13504
                     /locus_tag="VFU15_00545"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEU4716285.1"
                     /translation="MKKTTAIILAAILLSASCSEEKKKDKLSAELKTKKEAFENLRRE
                     ITDLQMQLDKLDTAGSDKGKPVNVMPLVYTRFTHSIDIQGRVDADESVSVGPQIPGLV
                     TRVYVHAGDKVAAGQVMAETDNAAMVQQLAALQAQRDLAKDVYTRQQNLWAQKIGTEI
                     QLLQSKTQYEALDKQVSAMQEQVDMTRIKAPIAGIVDQVNMKTGEMAAIGFSNIVLVN
                     TNSLRVKAEVAEGYLSQVHTGNVVNIYFPDANKTIEAKISYSGRMINKVNRTFNVEAV
                     IDPNEPDIVPNMIAVLKINDYSNDSALVAPLSCIQESSGGKHFVFIAVKNKEGQMIAE
                     KREVTYAHTYNGMAEITGGLSLNDMLITDGSGDLNAGDMIDVKK"
     gene            13536..>13982
                     /locus_tag="VFU15_00550"
     CDS             13536..>13982
                     /locus_tag="VFU15_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018476621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEU4716286.1"
                     /translation="MLEKIKEFKPTSWSINNRTSIFLLCIFLTLAGLLYYKKLPKESF
                     PEVKVPTIYVMTVYRGASPTDMENVVAKPLEKQFKAISGVKKITSNSIDNFCNVIVEF
                     NTNVKTDVALQKVKDAIDKAKKDLPQKLDQGPDAQEINFSEFPIMSI"
BASE COUNT         3559 a         3753 c         3654 g         3016 t
ORIGIN      
        1 agttgtaatt cgtctgcttg cgtgcatcga tgatggcata atcaccgctg cggatatcct
       61 gcgcgtacac cggtgtgccg tcatccgccg gatcatcagg atcgagcggt gcgttgtaat
      121 aaggtccgta agcgatcggc cagtcgccgt actgctcgcg gttcaggtaa gaaagaaggc
      181 tgatcgcgtt ttccgggttg ttctcgtcca tcggcgtatt cgcttttgaa cggatcacga
      241 ggataaggaa agtggaataa cccatcagga aaacagagaa ggaaacgagg atggtattga
      301 gcagaacgcg gttatcgcgg atcttgtaaa tgaggtatcc cgcaccggca gcggtcagca
      361 ctgacatcag cactttgctg tacgcgaacg ccccgatgac agtgaacagg aaaaacgtcg
      421 aagcaacgac gacaaaattc cggaagaggt tgcgtcctcc tttcatggaa tactgcagac
      481 ctgtgatgat cattccgaga agaagcgcga aatagatgat ggttccgctg ttgaagggaa
      541 ggctgaaccc gttcacaaaa agcagctcgt aattggctgc ggtcgatacc actcccggga
      601 tgatgccgtt ctgcaccacg cccagcaatc ccaccgcgac cagcgacgtg atcagcaggc
      661 cggaacgtgt cggctgatgt tttttgaaat agtaaatgaa gacaatggaa gggatcgtga
      721 gcaggttgag caggtggaca ccaatggaaa gcccgatcat gtaagcgatc agcaccagcc
      781 agcggtcggc gcgctccgaa tcatccgcgt cccagcgcag gatggcccag aaaacgagcg
      841 cggtgaaaaa ggaggacatt ccgtaaacct caccttccac ggcagagaac cagaaggaat
      901 cagagaatgc gtaggcaagc gagccgatga ccgcgcttcc gaaaatcgcg tacatgcgcg
      961 agtcggtaag ctcctgcttc atggctttta cgagcctttt gccgagcagc gtcatggacc
     1021 agaacatgaa caggatggtt ccggcgctgg ccagggccga catggtgttc acgcaggcgg
     1081 cgatgtgcgc gggatctttg gcaaacaacg agaagaaacg accgatgata aggaaggtag
     1141 gcgctcccgg ggggtgaccc acttcgagct tgttgcagca ggcaatgtac tcgccgcagt
     1201 cccagaagct ggctgtaggt tcaatacagg aaatgtagac gatggcggcg accaggaaaa
     1261 tgatccagcc gagaacgttg ttcagcagtt tatacctcgc tgtcatggaa ttgaaaataa
     1321 agcgtttgtt ggtgggcgaa gatagggaat tcggaattag agggggtgtt aattcaaacg
     1381 gagctttgcc aaaattttgt gaaaaatcag aatgtttata cttttgtgcc cctttggcgc
     1441 ggattcgcgc ggattggccg atggtgtaac tggcaacacg tctggttttg gttcagaaga
     1501 gtccaggttc gagccctggt cggccaacag gaaaaaagtc tctctttcac gggagacttt
     1561 tttattggcg ttctgctgat gctggttcgg gctccgtctt cattacacaa aacatctatc
     1621 ccatttcctt cagcatacgc acggtcatta tgcccagcaa taagataaca gtgccgatcg
     1681 cgatccaggt aaaaagttta ttctcatacc agggtgttgc ggaagaagac gcgatcgctt
     1741 ttgccttcac ccgtgaagag ccgggtgtcg tatccgcaag tacgccggca gaaacttttt
     1801 ccttcgcggt atcgttccgt ttcccggccc ggatgaccgg cggttgccgg ggcacttttt
     1861 cgcgaaagta gcggaggtcg taagcaggaa agcccattct ttcgtcgccg aaataaattg
     1921 catagctgtt ccccttttcc agctccgcaa cgatgtaatg cttcacctgg aagccggcca
     1981 ccgaatcgat gacgagcggc tggtcatctt cattcttcac gacaggcagc agttttttca
     2041 cacgcgccga attcaggttg atctcgttcg aattgaaaga agaaagaatg gtcgacgaaa
     2101 gttccgtctg ctgcataact gttcttccgt ccgccggcac ttcgtctatc ctgtacagcc
     2161 gtgccgcgcg ataataatcg cggttacttt tttgggcatg cgcgtaaaac ttcaggtgat
     2221 cgacgaattc cgtatctggg aatgtgacct ggtaatagct ttcgcggtga tgtgcgaaca
     2281 gcatccgcgg ctccatcttt tttcgacgac ctggtaagtg acgaaatgtt cgcatgcata
     2341 atattccggg aagacccgcc aaagacatct ccacaggagc attgtccgtc gctgaaagat
     2401 cttcttcccg cccggtcgct cattggccat gaaatcctgt gggagagccg gtttcccggt
     2461 tccgtccatt gcttttatga gttccggttc ctattttcac aaaaaaaacc ccattatgat
     2521 catcgtcttc ggcatccgta aattttccat ctacacgttc tcaccttccg agctcggctt
     2581 caccgatcct tcgtggaacg gcatccagtt caaggtgctg cagcagtatt tccatctcat
     2641 gtggattccc ttttttcctg tcgggaagaa atgggcgatc agcaaaccgg acggcaaact
     2701 gtacgaagtt ccgcacaaca ttctcggtca cctcggcgac ccttcggcga aagtgagaac
     2761 accctggtac gcattcgccg ggcttatcct ggccggtgtt gcgggcatca tttccggcat
     2821 cagcaacatg atgtaagcgg gaaagcgcgg gttaattaac aatccgtgtt tcacaatatc
     2881 ccgtcttccc atacgaaaca aggcgcgtac gcgttctaat tttacgttgg ccttgtcatg
     2941 tctgaaaacg ctgaaattcc cgccgtaccg gaagtccgca ggccgctgtg gaaaaagatc
     3001 ctgcgcttca cgctgtggac cggcggtatt ttcgtctttc tcattctcac gctttccgtt
     3061 ctcacgtgga tcttccgcga cgatatcaag aaatacgttt tcgcggaagc ctgcagccgt
     3121 gtgaaacccg agatcgtcgt gcagccgtcc gacatcgacg tatacattat ctccagcttc
     3181 cccgatgtca cggtacagct gaacaacgtc gccgccatcg acacggcgca tcacgtgaag
     3241 aacgacacgc tgctgaaagc cgggcggatc gagcttgcct tcaacatgct cgaccttttc
     3301 catcacaact acacgatccg caacatcacc atcgagaacg gcgcgctgaa cctttgggac
     3361 gacgcaaaag gaaacgacaa ttaccacttc ctgaaagaag acaccgcgaa aacgaccgat
     3421 acttcgcacg tggattttgc gctcgagaaa gtgcagctga aaaatatggc ctgctcctac
     3481 cgcgataaaa agagcggcga ctattactcg gttacgttca cggatatttt ttttaaggga
     3541 aaattcacag aaaagaattt cgggttcagc tccgatgcgg atttcctcgt caacaaactg
     3601 ctgtacggaa aaaattcctt tttcgaaggc gaccggggaa atttttccgt cgacatggac
     3661 atcgaccgca cgaccaatac ctactccatc cgaaagacga atattgcgat cgaaaagctt
     3721 tcgctcggtg taaccggtag cgtcaccgaa acggggaaaa aatacgcgac ggatctcacg
     3781 gtaaaaggcg agcaggtgga gcttacgtcc gtactttccc tgctgccggt gtcgtatcac
     3841 aagaacatcg ctgatttcga aagcaccggc gaatttttcg tggacggaac cgtgaagggt
     3901 atctacagcg attcctcgct gccggtgatc gattcgaaat tcggcatcag gaacggaggc
     3961 acgctgaaac gcaggtcggg caaaggcgag ctgcacgaca tttcgctcaa cggaacattc
     4021 accaaccaga aaggaaagga cggtcttgtc atttcttcgt tcaaggcttc cggcgcaaaa
     4081 agcattttca gcggttcttt ctccgtcctc ggttttgccc agccgaaata cgatgcaaag
     4141 ctttcgggcc gcatcgacct gggcgagctg tatgatatcc tgcaacccga cacactggaa
     4201 agcgcatccg ggaccgtgaa catttccctc gaaggatcgg gaaaaccgtc gaaagcggga
     4261 aaattcacca ttgctgattt ccggaaattc cgcacgcagg gaaatatggg tctcgccaat
     4321 gtggcggtga aaatgaaagg cgcaaaatcc ccggtcgaca gcatcaacgg gaatctttcc
     4381 ttcgacggga ataacgtagg catttcacac ttcaccgcgc gcagcgaaaa aagcgacatc
     4441 gtgatcgacg gaacggtgcg caacctgctc gggttccttt tcacgaaaaa agaagtgctg
     4501 gacgtttcgg gaaaattttc ctcgaacaat ctcgacctga atgccatcct cgcggaaaat
     4561 tccggggaga cgggaacgac cgacacgacc tatcacctcg ttctacccga gcggctgaag
     4621 ctcaacctcg atgccgccgt gaagaaaatc gccttccgtc ttttcgaagc ggaaaatgtg
     4681 gccgggaaag tttccctttc cggtcagcgc ctcattgccg atcctttaag cctgaacacg
     4741 atgggcggat cgatatccgg cagcggaatg atcgacggca cgatggaaga ttccctgctc
     4801 atgacgtgca acgcgaacat cagccaggtc gacatcacga agctgttcta tgaaatggaa
     4861 aatttcgggc agggtcccga tgacgaagtg atcaccgata agaacgtgaa aggaaggctc
     4921 acttccactg tcaatttcgc ctcgctgtgg ggaaatgatc tcagcgtaaa tgagaagaaa
     4981 atattcactg atgcggatgt cacgatcgag cagggcgagc tgatcgattt caagccgctg
     5041 agcgtgctgt cgcgtttcat caagctcgat gatctcatgg acgtgcgttt caaaaatctt
     5101 cacaacacga tcacgatccg gaaccgcgtt atttccatgc cgaaaatgga gatcagctct
     5161 tcggcgatga atgtcaccat gagcggcacg cacgatttcg acaacaacgt ggattatcat
     5221 tttatcgtcg acctcgacga actgcgtgcg aacaaggtga agaaatcaaa gccgcagaat
     5281 tcagatttcg ggcaggagat cgacgacggc ggtcaccgtt accggttgtt catcatcatg
     5341 aaaggaccga tcgatgatcc ggatgtgaaa tacgactcca aaggattttt ccagcagaaa
     5401 aaagaagagc tgaagcagga aaaacagaac ctgcggcaac tgcttcatga cgaatgggga
     5461 attttcaggg acgacagcgc aggatcaact gatccaaaaa aaaaaggaga ccggaagaca
     5521 gacgacggag ccggaaaatt catcctccag cagagcgaca aaccagcgaa aacaaagaag
     5581 aaaccgaagg accaggacct tgatgacagc ggggattact gaccgggtgc cggtaccgtt
     5641 cccgggtaat taaattttcc ctggttcatt tgacgaggtc gaaagtggac taaattgctg
     5701 tggcaaaata atcccatgag atcgctcgtc ctctttatca cagcgctgac gctgagcttc
     5761 agttcgcccg cgcaggtttt cgaaatgacg tacggaacgt ccggcagcga agacgcttac
     5821 ccgatggaag cccttccgga cggcgggttc attctcgcgg gaagctcgtg gggaaacggc
     5881 gccggcggaa aagacgtcat gcttgtacgc acggattcgc tgggaaatct cctctggtcg
     5941 aaacaatacg gcggcagcgg cgacgaaacc gcgctgtaca tcatccgcct cagcgacgga
     6001 ggtttcgccg tctgcggtga atcattcagc gatgatgtga acggggatgc ttttctcttc
     6061 cgcacggatt cgctgggaaa tctttcctgg tggaaaaatt acggagcggc gaattacgat
     6121 atcgcctatt ccgtcgtcga gctgcacgac ggaaacctcg ccatttccgg tctcactgaa
     6181 acaacgccct cccagtacga tgcattcgtt gcagaaacag acgcgacagg tgatacgtta
     6241 tggacgcaaa ccatcggtgg tcccggcatc gaccacgccg tacagatgat cgaaacgagc
     6301 gacgatgcga ttctcttttg cgggaaatca ctttcctatg gcgcaggaag ctgcgactgt
     6361 tttcttgtga agatcgatga gaacggcgac acgctctgga caaaaaccat cggtggtgcc
     6421 ggctgggatg aaagcatgga cgtgatcgaa cgccccgacg ggttcgcgat ctgcggcgga
     6481 acgaccagca gcggttcggg cagctacgat ttcctgctga tgaaaaccga tctcagcggg
     6541 aatctccagt gggcaaaaac ttacggaggt gcaaagatcg aagcgagcta ctgcatcatc
     6601 gacgtggccg gaacgggtta tgcgtttgcc gggtacaccg aaactttcgg gcccgggcac
     6661 agccgcggaa cggacagcgc gaacgcgatg ctcatcctca ccgattattc cggcgacacg
     6721 atctgggcag cctcctacgg cggactgctg aaggaagaat gtttttccgt gcgacaaacg
     6781 cattcgggcg ggtacgcgat cgcgggatac acgggaagct tcggcgacag cctgcaggcg
     6841 tatcttttcg ctaccgatcc gcagggcaac accggctgca acgacaggcg cgcacatccc
     6901 gatgtagcga acgcgaattt cgcctgcacg tcttacgcat acgacgtttt tcattcgctg
     6961 catgagaatt cattcgccgc tgccgaagcg ccgctttatt tttctccccg gcaggtttgc
     7021 ggcgggccgt tgtcttccgg gaacaggaac gaaaccggtc cgcttcttct gtacccgaac
     7081 ccggctgccg gttttgtaac agtctgcacc gatgaaaaag aaacgacgca aatcacgctg
     7141 aaagacgtaa cggggaaaac agccggaact tatttcaccg gtgcgaatgg caatgtcgtt
     7201 atcaatacca atggtcttgc agcgggaatt tatttcgttt ccttcgtgaa aaaagacgga
     7261 agctgcataa gccggaaatt catcatacag taaaaacacg gaaatcttcg gttaacggtt
     7321 ttcgacctgg tgtttatcgc gtgttgccag ttgttctctg tgaacttccg tgaaacaagt
     7381 tctgtgttcc cggtgtttta aaaattcgga aaaaacacga agacgtgaaa gaaaccctta
     7441 caagccctgg ctgagccggg aacagcttcc gggaagggac aacgtttttt ttcactacct
     7501 tttcatttca aagcaaacac taactcaacc caaatgaaaa aaatctacct ctttcccgca
     7561 cttgcgggcg cagtcatgtg tgtcacggcg aacgcacaaa catcgacact cttcaccgaa
     7621 aacttcaacg ggaacactta cggattcacc ctgaacacaa ccgatgcaag ctccacggcg
     7681 acaggcgcga acacctgggt ggtaaacaac tcttacaccg gcggaagcgg aaccgcgacc
     7741 tgccagagct tttccgttcc gttcaccatc aacaacaccg cgcaggagcc ggccggcatc
     7801 accggtcagc cgacgagcaa atacatgcac atactttccg actccgcgca gatgcagggg
     7861 atcacgtgcg gatgctacat ggccgcagac ggattctgca caatggcgga atcaatgttc
     7921 agcaaaatgt cttccgacat cagcaccacc ggttactcgg gtgtttcgtt caacttctgg
     7981 tggctgtgcg gcggaagcgc caacggttac ggcctcgtgt attacagtac gaacggcgga
     8041 acaacgtgga ctgcgctgag cacacagtac aacaacagct cttctgcatg gacgcaggcc
     8101 agcgtcacca acgcggcatt cgacaaccag gcgacgcttc gtttcggctt ccagttcgtg
     8161 aacaacacga cgttcaacgc agccgatccc ggcttctgca tcgatgacgt gacgatcacc
     8221 ggcagcacgg caacaggcat ggcggaaaat aatctcgttt cttccgtcaa tcttttcccg
     8281 aatcctgcga acgatcacat tacgctgacg tggaacggcg ctgttcccgc cgacaacaaa
     8341 atgctgatca gcatttacga tgtaacggga cagctcgtga aaacggaaac gcgcgatttc
     8401 gcggtccgtt cggaaatgga tgatctcgcg cttgcgaatg gcatctatac attccgcgta
     8461 cagtgcggag agcaaacagc catctccaaa atcacggtcg cggaataagt cggcgaaaat
     8521 tctccaatga aaaagtcccg gccctgaggc gcgggacttt ttgatttaaa cgacctgccg
     8581 gttaaaactc cgcgttcttc ggcgtgcgcg ggaacgggat cacgtcgcgg atgttggtca
     8641 tcccggtgac gaaaagcagc aggcgctcga agcccaggcc gaagccgctg tgcgggcacg
     8701 tgccgaactt ccgtgtttcg aggtaccacc acaatgattc ttcgggaatg ttcagctcct
     8761 tcacacgtgc gtacaatttg tcgtgatcat cctcgcgctg cgagccgccg acgatctcgc
     8821 cgatccacgg aaaaaggata tccattgcac gcaccgtgtt cccgtcatcg ttctgcttca
     8881 tgtaaaacga tttgatcgct ttcgggtaac cggtgacgat cacgggtccg ctgaaatgct
     8941 cgacgagata attctcatgc tctttctgca ggtcgtgtcc ccattcgggc gtgaactcga
     9001 attttttcgt gcccatcgct tctttcagga tgtcgaccgc ttcggtgtag gaaatgcgcg
     9061 cgaacgggtg ttcgatcacc gactgcaggc gttccagcaa ttgattgtcg tacgtgtcgt
     9121 tcaggaactg gatatcgtcc ttgcaatgat gcaacgcgta gctgacgaga tatttcagca
     9181 tctcctccgc gaggttcatg ttgtcgttga tatcgtagaa cgccatttcc ggttcgatca
     9241 tccagaattc cgcgaggtgt cgcggcgtgt tggaattttc agcgcggaac gtcggaccga
     9301 acgtataaat aagtccgagc gcaagcgcag ctgtttcacc ttcgagctgg ccggaaacgg
     9361 taaggttcgt ttcacggccg aaaaaatctt cctcgaaatt caccttgcct tctttcgtac
     9421 gcggaagatg atccaggtcg agcgtggtga cgcggaacat ttctcctgcg ccttccgcat
     9481 cactgccggt gatgatcggc gtatgaatat tgaagaagcc gcggtcgttg aagaatttgt
     9541 ggatggcgaa cgtcatgtgg tgacggatgc gcagcacggc gccgaacgtg ttcgtacgcg
     9601 gacgcaggtg cgctttctcg cgcaggaatt ccagcgggag cgtttgcgtt tttttatctt
     9661 tttcatcttt cccgcgtttc tgcagcgggt aagtttccgg atcgcagtcg ccgaggatct
     9721 cgatcgtcgc ggcctgtatc tccgacgact ggccttttcc ctgtgaagcg accagcttcc
     9781 cggtaaccga aatgcacgcg ccggtggtaa cgcgtttcag caacgcttcc tcgaacttcg
     9841 ccacatccgc cacggcctgg atattcttta tcgtcgatcc gtcattgatg tgaatgaact
     9901 gcacgtattt atttccgcgg gcgcttctca cccatcctga tacctgtacg tcgcggtcca
     9961 gctctgtcga cagaagcagg tccctgattt tttccattgc cattatgctc atttttattt
    10021 gggagaacaa aaaaacgaaa attcaacggg cgaaccgcat ttaaggatcc gcccggagca
    10081 gctcatcgcc gtttcccgtc actttccgcc ccggcaagtt ccattcatcg atccgcggaa
    10141 aatatccgcg tttccatcgt ttccattatt aataattgac aatcaataat atacaggcat
    10201 tccggcaagc cggaaacatt gatttttcct ttttttcatc gaagaaactc tctgatcaaa
    10261 aaatgtttcc atagttttgc cgcgcaaatt tcaataatgg aaaccaaaga aagaatcgtg
    10321 tcggcggcct tcgagctgtt tttccgttac ggcatcaaaa gcgtgacgat ggatgacatc
    10381 gcgaagcatc tttccatgtc gaaaaaaacg atctaccagt ttttccgcga caaggatgaa
    10441 gtggtgcata cgcttatgga acgtgcgctg gaggaagacc accgcgattt cgagaacatc
    10501 tcgaaaaatt cggagaacgt cgtggaatcg gtgttcgtga tgatgaaaaa aatgcacgac
    10561 atcttcagca acatcaaccc gaatattttc tacgacctgc ggaaatacca tccgaaaaca
    10621 tgggacctgt tccaccgctt caaaacggaa ttcgtgatgg ggatggtgga aagcgagctg
    10681 gagaaaggga aaaaggaagg tcttgtgcgc gctgacatta actcgcgcgt gctttcgcgc
    10741 ctgcgcggcg aagagatcga aatgggattt gacccttcgc ttttcccgcc cgacaaattc
    10801 cgcatactcg acgtgcagct ggcgctgacg gaacatttcc tttacggcat ctgcacatta
    10861 aaaggtcaca agctcatcaa caaatacaaa caaattgtgg aggaagaata aaatattcgg
    10921 cccctcctta acgactacca gacgtccatt caaccgctca aagttccctt tatgaaatac
    10981 gccaggatta cccttacgtg gatcgccgca gctgcgatcc cggccttact ctccgcgcag
    11041 cagacagaca cttccagcta cgcgttttcc ctccagcagt ccgttgattt tgcactgcag
    11101 cacaacaccg cttaccagaa cgtcctgctc gatgaaacgg cggcgacggc aaaagtgaag
    11161 gaagtcaccg gcatcgggct cccgcaggtc aacggcagct tccaggtgca ggactttctc
    11221 gagatcccca catcgatgat cccgggagaa tttttcggcg gtcccccggg ctcttacatt
    11281 ccggtcaagt tcggcacgaa gtacaacgcg acagcggggg ttacggcttc acagctcgtg
    11341 ttcagcggac cgtggctcgt gggattgcag gcggcccggc tttaccagga tcttgcgctg
    11401 aaaaatgccg accgcacgaa gatcgaaacg tcgtgcgatg tgacgaaagc gtattacaac
    11461 gccctggtga cgacgaagaa aaaagagctg ctcgacgcga acctcgaccg cgttaaaaaa
    11521 gtgatggacg acacgaaggc gttttacgat aatggtttcg tggaaaagat cgaggtcgac
    11581 cggatcactg tcacctacaa caaccttgtg acggaagtga gcaatgcaca gcgcctgatg
    11641 gacctttcca tggtgatgct caagtaccag atgggaatgg accagtcggc aaagcttacg
    11701 ctgaccgaca acatcgagaa catctcgttc acaccggcag aaacgcagaa cgggaaattc
    11761 gattacacga aacgcgtgga gttccagctc atggacatgc agctccgcgg caaagagctt
    11821 gcgttccgcg cagaccgcat gggctatttc cccacggtcg cacttttcgg ttccgcgcag
    11881 gcacaggcgc agcgcacgaa attcgatttc ctcgacggca cgagccagcg ctggttcccg
    11941 gttgtcatcg tgggattgca ggtgagcgtt cccattttcg acggcttcca gcggcattac
    12001 atgatccagc aggaccgcgt cgggatcctc caggcgcaga acgacctgct gttcatgcag
    12061 cgcacgatcg acatgcagca ggcggttgca aaaactacgc tgaccaacag cgcggcgacg
    12121 ctcgcgcagc agaaagcgaa cctcgatctc gcgcaggaag tattcgacgc cgccaaaacg
    12181 aaatacgacc agggcgtcgg atcgaacctc gagatcatca acgcgcagac cgcgctcaag
    12241 gaagcgcaga cgaattactt cgacgcactc accagcgccg tcatcgcgaa agtcgattac
    12301 gacaaagcca ccggcacatt caacgttaaa taaaagacac tattcattcc gctccaaaag
    12361 ccattttatg aaaaagacaa ctgccattat tcttgccgcg atccttcttt ccgcttcctg
    12421 cagcgaagag aaaaagaaag acaagctcag cgcggagctc aaaacgaaaa aagaagcgtt
    12481 cgagaacctg cgaagggaga tcaccgatct ccagatgcag ctcgacaagc tcgacaccgc
    12541 cggttctgac aagggaaaac ccgtgaacgt catgccgctc gtgtataccc gcttcacgca
    12601 ttccatcgac atccagggac gtgtggacgc ggatgaaagc gtgagcgtgg gcccgcagat
    12661 ccccggtctc gttacgcgcg tctatgtgca tgcaggcgac aaagttgctg ccggccaggt
    12721 gatggccgaa accgacaacg ccgccatggt gcagcagctc gccgcactcc aggcgcagcg
    12781 tgatctcgcc aaagacgtat acacgcgcca gcagaatctc tgggcgcaga agatcggaac
    12841 agagatccag ctgctccagt caaaaacaca atatgaagcg ctcgacaagc aggtgagcgc
    12901 gatgcaggag caggtcgaca tgacgcgcat caaagcgccc attgcaggga tcgtcgacca
    12961 ggtgaacatg aaaacgggcg agatggccgc gatcggtttc tcgaacatcg tgctcgtgaa
    13021 cacgaacagc cttcgcgtaa aagcggaagt ggcggaaggc tacctgtcgc aggtgcacac
    13081 cgggaacgtg gtgaatattt atttcccgga tgcgaacaag accatcgaag cgaagatctc
    13141 ctactccggc cgcatgatca acaaagtcaa ccgtacgttc aatgtcgaag ccgtgatcga
    13201 tcccaatgaa ccggacatcg ttcccaacat gatcgccgtg ctcaagatca acgactacag
    13261 caatgattcc gctcttgtcg caccgctcag ctgcatccag gaaagttcag gcggaaaaca
    13321 tttcgttttc attgccgtga agaacaagga aggacagatg atcgcggaaa aacgcgaagt
    13381 gacatacgca catacttata atggcatggc ggaaatcacc ggcggacttt ccctgaacga
    13441 tatgctcatc accgacggaa gcggcgacct gaatgccggt gacatgatcg acgtgaagaa
    13501 ataaccgaag aactcaccgc tccaaacgaa attttatgct tgagaaaata aaagaattca
    13561 aacccaccag ctggtcgatc aacaacagga cgtccatttt cctgctgtgc atcttcctca
    13621 cgctcgccgg tttgctttat tataaaaagc ttccgaagga aagcttcccg gaagtaaaag
    13681 ttcccacgat ctacgtcatg accgtttacc gcggcgcttc gcccacggac atggagaacg
    13741 tagtggccaa gccgctcgag aagcagttca aagcgatctc cggcgtgaaa aagatcacca
    13801 gcaattccat tgacaatttc tgcaacgtca tcgtcgagtt caacacgaac gtgaagaccg
    13861 acgtcgccct gcagaaagtg aaagatgcga tcgacaaagc gaaaaaagat cttccgcaga
    13921 agctcgacca gggcccggat gcgcaggaaa taaatttttc ggagttcccg atcatgtcca
    13981 tc
//
LOCUS       DASTSF010000019         3194 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1321948,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000019 DASTSF010000000
VERSION     DASTSF010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3194
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1321948"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..246
                     /locus_tag="VFU15_00555"
     CDS             <1..246
                     /locus_tag="VFU15_00555"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEU4716287.1"
                     /translation="YTILAAMKSQPEMKVEIDGHTDSDGDPLANQKLSEDRAKAVADF
                     FIENGIDPSRISWKGFGSTRPVSDDKSRNRRVEFVFL"
     gene            300..1379
                     /locus_tag="VFU15_00560"
     CDS             300..1379
                     /locus_tag="VFU15_00560"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEU4716288.1"
                     /translation="MRTSLLLFLLFFFMNGPAQNLIINPGFETNGVNKTNGGDCFQLN
                     GVPGWFNPSDGSTDYFTSNPKDPMNAFGMNFFGGPCTPHSGKCFSGFIPYLSDREYRE
                     YEAGELSAPLEAGKYYEISFSIMLGPLSEFKIDQLDVFFMNEKGAHVPNSGPYPCGEF
                     YHIDSLLGKTQLGKWLDVKLDIVAQGGEKFFVLGNLSNDKHTKAEQITSTVSPNHNYA
                     YYYFDDASVVPLNAKPKNVREMTVVLDSTLFEPGKKLVLSTINFDLNKATLRPESYKI
                     LDQVVQQMKNKPWLKVEIDGHTDLTGKQDANQKLSEARAKSVADYLISKGIPEYRITT
                     KGYGSSRPVGADDAKNRRVEFIFHE"
     gene            1556..1744
                     /locus_tag="VFU15_00565"
     CDS             1556..1744
                     /locus_tag="VFU15_00565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716289.1"
                     /translation="MDQLKLHSEIQKRAAQNKPKNENRNLTRENSPRPTGFTPDEMLL
                     IGHSRNSRKQFFYSGSRD"
     gene            complement(1741..>3194)
                     /gene="bshC"
                     /locus_tag="VFU15_00570"
     CDS             complement(1741..>3194)
                     /gene="bshC"
                     /locus_tag="VFU15_00570"
                     /inference="COORDINATES: protein motif:HMM:TIGR03998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bacillithiol biosynthesis cysteine-adding enzyme
                     BshC"
                     /protein_id="HEU4716290.1"
                     /translation="AEQLSFDDEKRQTLCRVIRQQYTSSGIDVPADLISALEKKTSVT
                     ITTGHQLCLFTGPLYFIYKILAAVRIAQEQSRKLGREVVPVYWMASEDHDFDEIASVN
                     VFGKTITWTKPAEGAVGHLRTDLLGEVMAQVKAVCGESENAKELLSVFETAYAPGRSL
                     ADATRILVHRLFEGKILVLDPDDVSLKKYFVPHFHKDIFENTFAENVQASLEKLKTAG
                     FDAPVNPRAINVFYLGSDFRRRLEKTGGKYMVDGTAVSFTPGELEQELASHPERFSPN
                     VVLRPLYQQVILPNISYVGGPAEISYWLEYRSMFEAAHVHFPVLQPRFHALVIDKNSA
                     SRMEKLGITAEQLFAGADVLVKRFIADNAGVSVSLEKEKEKLAVLYAEIAAAVAKTDV
                     TLKQSAEAELQKALKGLETLEGKMMRAAKQKEETNLAQLHKLHEKLFPAGGLQERYDN
                     FIPFFLGQGYAFFRKMEEAVEFPVSGICIVFPD"
BASE COUNT          802 a          857 c          807 g          728 t
ORIGIN      
        1 tacaccattc tcgccgcaat gaaaagccag ccggaaatga aagtggagat cgacgggcac
       61 accgacagcg acggagatcc gctggcgaac cagaagcttt cggaagaccg tgcaaaagcg
      121 gtggcggatt ttttcattga aaacgggatc gacccttcaa gaatttcgtg gaaagggttt
      181 ggaagcacac ggccggtcag tgacgacaaa agcaggaacc gccgcgtgga attcgtattt
      241 ttataaccag gtgccgtgta acttttcagt atgggcggca ttatgaagga aaagtcccca
      301 tgagaacgtc gctgctgtta ttcctgcttt tcttttttat gaatggtccg gcgcagaacc
      361 tcatcatcaa tcccgggttc gaaacgaacg gcgtgaacaa aacgaacggg ggcgattgct
      421 tccagctcaa cggcgttccc ggctggttca atccttccga cggcagcacg gattatttca
      481 cttcaaatcc caaagacccg atgaacgcct tcgggatgaa tttcttcggc ggtccgtgca
      541 caccgcacag cgggaaatgc ttttcaggtt tcattcccta tttgtccgat cgtgaatacc
      601 gcgaatacga agccggtgag ctcagcgccc cgctcgaagc cggaaaatat tacgaaatat
      661 ctttctccat catgctcggt ccgctgagcg aattcaagat cgaccagctg gatgtttttt
      721 tcatgaacga aaaaggcgcg catgtgccga attccggtcc ttatccctgc ggggagtttt
      781 accacatcga cagcctgctg ggaaaaacac agctgggcaa atggctcgac gtgaagctgg
      841 atatcgtggc gcagggcggt gaaaaatttt tcgtgcttgg caatctttcc aacgacaagc
      901 acacgaaagc ggagcagatc acgtcaacgg tttctccgaa tcataattac gcttactatt
      961 atttcgatga tgcgtcggtt gttccgttga acgcaaaacc gaagaacgtc cgcgagatga
     1021 cggtcgtgct cgattcgacg ttgttcgagc ccgggaaaaa actggtgctg agcacgatca
     1081 atttcgacct gaacaaagcg accctgcgac cggaatcgta caagatcctc gaccaggtag
     1141 tgcagcagat gaaaaacaaa ccctggctca aagtcgagat cgacgggcac accgacctca
     1201 cggggaagca ggacgccaac cagaaacttt cggaagcgcg tgcaaaatcc gttgccgatt
     1261 acctcatctc gaaaggaatt cccgaataca ggatcacgac aaaaggatat gggtcttccc
     1321 ggcccgtggg tgcggacgat gcaaaaaacc gccgcgtgga atttattttc cacgaataaa
     1381 caatccccgg tcgctttacg cagccttaac ctgcagggtt ttggcgctca attcgttgga
     1441 aatatcacgg gattcccggt taacacaagt gtcatttgcc ggtaagattg aaaggatgtt
     1501 gcagaagtac atttggattg aacaaaccat ttccttccac caaaactttc cggccatgga
     1561 ccagctcaaa cttcacagcg agatacagaa gcgcgcagca cagaacaagc cgaagaacga
     1621 gaacaggaat ctcaccaggg aaaattcacc gcgccccacg ggtttcactc ccgacgagat
     1681 gctgctcatc ggtcactcga gaaattccag gaaacagttc ttttattccg gcagccgcga
     1741 ttaatccggg aaaacgatgc agatgccgga gacgggaaat tccaccgcct cctccatctt
     1801 cctgaaaaac gcgtaaccct gcccgaggaa gaacgggatg aaattgtcgt aacgctcctg
     1861 caacccgccg gctgggaaaa gtttttcatg cagcttgtgc agctgggcga ggttcgtttc
     1921 ctctttttgc ttcgcagcgc gcatcatttt tccttcgagc gtttccaatc ccttcaatgc
     1981 tttctgcagc tccgcttcgg cgctttgctt gagcgtgacg tccgtttttg caacagcggc
     2041 agcaatttca gcgtacagca cagcgagttt ttctttttct ttttcaagac ttacagaaac
     2101 gccggcattg tccgcgatga atcttttcac cagcacatcc gcaccggcga acagctgctc
     2161 cgccgtgatc ccgagctttt ccatccgcga tgcagaattt ttgtcgatga ccagcgcgtg
     2221 aaagcgcggc tgcagcacag gaaaatgaac gtgcgcagct tcgaacatgc tcctgtattc
     2281 gagccagtag gaaatttctg cgggaccacc gacgtaggaa atattgggaa gaatgacctg
     2341 ctggtacagc gggcgcagca cgacgttcgg agaaaaacgt tccgggtgcg aggcaagctc
     2401 ttgctccagt tctccgggtg taaacgaaac cgcggttccg tcgaccatgt attttccgcc
     2461 tgttttttcc aggcgcctgc ggaaatcact cccgaggtaa aatacattga tggcacgcgg
     2521 attcaccggt gcgtcgaagc cggcggtctt cagtttttcc agcgacgcct ggacgttttc
     2581 cgcgaaggtg ttctcaaaaa tatctttatg aaaatgcggg acgaaatact tcttcagcga
     2641 aacgtcatca gggtcgagca caagtatttt tccttcgaaa aggcgatgca cgaggatccg
     2701 tgtcgcatcg gcgagcgaac gtccgggcgc ataagccgtt tcgaaaaccg aaagcagctc
     2761 ttttgcattt tcgctttcac cgcacaccgc tttcacctgc gccatgactt caccgagcag
     2821 gtccgtgcgc aaatgcccga cagcgccttc cgcaggtttt gtccatgtga tcgttttccc
     2881 gaacacattc accgaagcta tttcatcgaa atcatgatct tccgacgcca tccagtaaac
     2941 gggaaccact tccctgccca gcttccgcga ttgttcctgc gcgatgcgga ccgccgcaag
     3001 aatcttataa ataaaataca gcgggcccgt gaacaggcaa agctggtgac cggtcgttat
     3061 ggtaacggaa gtcttttttt ccagcgctga aataagatca gccgggacat caattcccga
     3121 agacgtgtat tgttgccgga taacgcggca gagcgtttgc cgtttttcgt catcgaaaga
     3181 aagctgttcc gccg
//
LOCUS       DASTSF010000020         6989 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1320433,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000020 DASTSF010000000
VERSION     DASTSF010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 6989)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6989)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6989
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1320433"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="VFU15_00575"
     CDS             <1..195
                     /locus_tag="VFU15_00575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716291.1"
                     /translation="DYPKHPAYVKTHWGREGETNYCLKLNELSTREQEIFVRDVRTML
                     ANNDLVFVDEWAECKGTPQN"
     gene            312..1928
                     /gene="pckA"
                     /locus_tag="VFU15_00580"
     CDS             312..1928
                     /gene="pckA"
                     /locus_tag="VFU15_00580"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017843083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HEU4716292.1"
                     /translation="MNEFGLKGKHADIAQLGLGNADTVYWNLSPAELVEETIDRGEGF
                     LCDTGALAIDTGEFTGRSPKDKFCVYDEITKDTIWWGDINIKFDPEKFDLLYNRVTAY
                     LGGKEIYVRDSYACADPRYRLNIRVVTEFPWSNLFANNLFLRPTEDEIENFLPEWHIV
                     CAPGFKADPKVDGTRQHNFAILNFTRRIILIGGTGYTGEIKKGIFTLLNYLLPQEKGV
                     LSMHCSANIGDKGDTAIFFGLSGTGKTTLSADPKRKLIGDDEHGWSDNTVFNFEGGCY
                     AKCVDLSKEKEPEIWNAIRFGALLENIGLKPGTRSVNFHDISKTENTRVAYPIDHISN
                     SIEPSVGNVPKNIFLLTADAFGILPPISRLTTEHAMYHFISGYTAKVAGTEMGITEPQ
                     TTFSACFGKAFLPLHPGKYAELLGKKLKADNSIRVWLVNTGWTGGAYGTGSRIKLSYT
                     RALITAALDGSLDKADFKTLPVFNLAYPTTCPGVPSEILNPRDTWHDKSAYDANAAKL
                     AGQFVQNFEQYASGVTREILEAAPQVAVSA"
     gene            2018..3346
                     /locus_tag="VFU15_00585"
     CDS             2018..3346
                     /locus_tag="VFU15_00585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716293.1"
                     /translation="MKKRVQLFFEAGHHNSRSVWPVLFGVILGLTCLRQHLVSNYNCE
                     LCADKAGYYMYLPASFGMGFHADAYPGNFDHDHGDGFHLDRQSNRVVTKFTCGVAILD
                     APFYALGAIAGRIFSPGTSPYSYFYLFFINIGAAFYVTAGMYFLRRWLDHYTGRITSF
                     LAVLAVFAGTNLYYYTLDESLMSHVYSFALFCFVLYAMKEYTLSHSFSHFIFFSVSLS
                     LAILIRPTNFLFGIFALLTDVKNPGDLKRKFSLFISFRNITAFLVIFFLVMLPQVIYW
                     KFAFGKYVVWSYEGEGFTNWNDPAFLTVWFSPQSGLFTYTPLLLVPLVFAVYMVRKKI
                     PNGVLVLAAFFIVSYLCASWHNPFFGVCNFGKRPFVEYLPLLMFPLALLFERFGSLPK
                     FRRNIVLVSVLFFIYYNQALFAAFNTCFPGSEWDWREFFHFLGRALPLIR"
     gene            3388..4050
                     /locus_tag="VFU15_00590"
     CDS             3388..4050
                     /locus_tag="VFU15_00590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716294.1"
                     /translation="MNYFPAIFCFITSLFFFSCSTSSPPGIKNTADTVVVPASKNKNT
                     PASAQDTSSVLDVHKLCQLADSFWTKTSGRSVHLSLQHDTGSSVQFYRTDMLEAVNPA
                     GNAKQEKFFVFEPGGNKDFSFSVITLTYPFIIDAMNAFSRLKKITGMNKAFGDMTPGL
                     TYENDYVLLSGKTIFWMNASCAYDRKSTGAISAFVLHSLKTEEPVDSIRCGCGQAQCD
                     TL"
     gene            4054..4764
                     /locus_tag="VFU15_00595"
     CDS             4054..4764
                     /locus_tag="VFU15_00595"
                     /inference="COORDINATES: protein motif:HMM:NF025028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY domain-containing protein"
                     /protein_id="HEU4716295.1"
                     /translation="MKHVHQAVRFICCATLLSFITSCGTQAGEKEPRPGGRKNDSSTG
                     LPADYKNYVPAANGFYRGKDGAFYILTRADDIRGEHYDTRPYFRKVNEVDPATFHDID
                     AYGIYAEDSFHVFEHFHMAEGDHIFLLDSADRNSFTVMGYRNAKDKHHVYSDNEALDL
                     DPSSLVIVDADSLPDGRVYIGCMKDKQRVYFEGEEQGNVDAPSFHLCSRRHDTVIFCD
                     KYRDYRSCDQCQGLENMK"
     gene            4838..5488
                     /locus_tag="VFU15_00600"
     CDS             4838..5488
                     /locus_tag="VFU15_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014083538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU4716296.1"
                     /translation="MIIAEQITKSYGSLQVLKGIDLSIRKSEVVSIVGASGAGKTTLL
                     TILGTLDKPDGGRLMIDGQELSGMNQKAAAIFRNKSIGFVFQFHHLLPEFTALENVCI
                     PAFIAKTPRKQAEENAKQLLDMFGLGDRLKHKPSELSGGEQQRVAVARAMINKPSVIF
                     ADEPSGNLDSVNSKHLHELFFRLRKDLGQTFVIVTHNEELASMADRKLTIKDGKVL"
     gene            complement(5589..6029)
                     /gene="rnpB"
                     /locus_tag="VFU15_00605"
     ncRNA           complement(5589..6029)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VFU15_00605"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(6056..>6989)
                     /locus_tag="VFU15_00610"
     CDS             complement(6056..>6989)
                     /locus_tag="VFU15_00610"
                     /inference="COORDINATES: protein motif:HMM:NF021179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2027 domain-containing protein"
                     /protein_id="HEU4716297.1"
                     /translation="TEGFEYNYPAAQLVPAEPVRTEPAKKQEETAPAEKNLSVSVPAN
                     TRFPDGIFLAFVPLNQHFPSASELEFRIINHSDYDVYYTISVKNGNSWICVESGALRP
                     HAERAVDTLTPQQADEWGQVKTDVLFFSGDDYVHVPPVSHVTKFKGVKFFRDSIYNEH
                     ALTGKKSWVTEVFLFDDERAEEEDAPKLTNEDIRRMMLEKEKNFKGTKTSVPHLKNQV
                     LEKEVDLHIEELLDNWAGMTNAQLIDVQLRKMQKELDAAIAAHLQRIIFIHGVGNGRL
                     KQEVRKILSTYKNVRFHDASFQRYGFGATEAEIF"
BASE COUNT         1580 a         1945 c         1772 g         1692 t
ORIGIN      
        1 gattacccca agcacccggc ttacgtgaaa acccactggg gccgtgaagg cgaaacgaac
       61 tactgtttaa aactcaacga gttatcaaca agagaacagg aaatcttcgt ccgggacgtt
      121 cgcaccatgc tggcaaacaa cgacttagtc tttgtcgacg aatgggccga atgtaaaggc
      181 acaccgcaga actaagcgcg ttttcattga cgccgaatcc gtaaatttgc tcctcattta
      241 ttcattattt cttaacccgg gaagaagggt ggaataacgc ttttttcttc cccgaaaaac
      301 aggcataagc aatgaacgaa ttcgggttaa aaggcaaaca tgccgacatc gcccaacttg
      361 gccttggcaa tgcagataca gtatactgga acctgtcgcc ggccgaactt gtagaagaaa
      421 caattgatcg cggggaaggt tttctctgcg ataccggggc tcttgccatc gacacggggg
      481 aattcacggg ccgctcgccg aaagacaaat tctgcgttta cgacgagatc acgaaagaca
      541 cgatctggtg gggagacatc aacatcaagt tcgatcctga aaaattcgat ctgctttaca
      601 accgcgttac agcttatctc ggcggaaaag agatctacgt gcgtgattcc tacgcctgcg
      661 ccgatccgcg ctacaggctc aacatccgcg tagtgactga atttccgtgg tcgaacctgt
      721 ttgccaacaa ccttttcctc cgcccgacgg aagacgagat cgaaaatttc cttcccgaat
      781 ggcacatcgt ctgtgcgccg ggattcaaag ccgacccgaa agtggacggt acgcgccagc
      841 acaatttcgc gatcctcaac tttacgcgca ggatcatcct catcggcgga acaggttaca
      901 cgggcgagat caagaaaggg atcttcacgc tgctgaacta ccttcttccc caggaaaaag
      961 gcgtgctttc catgcactgc tccgccaaca tcggcgacaa gggcgatacc gctattttct
     1021 tcggactttc cggaacgggc aagacgacgc tttccgcaga tccgaaacgc aagctcatcg
     1081 gcgacgacga gcatggctgg tcggacaata ccgttttcaa tttcgaaggc ggttgttacg
     1141 cgaaatgcgt cgacctttca aaagaaaaag aaccggagat ctggaatgcg atcaggttcg
     1201 gtgcgctcct cgaaaacatc gggctgaaac ccggcacacg cagcgtcaat ttccacgaca
     1261 tctcgaaaac cgagaacacg cgtgttgcct acccgatcga tcatatctcg aattccatag
     1321 agccttccgt aggaaatgtt cccaaaaaca ttttcctgct gactgcggat gcgttcggca
     1381 ttcttcctcc tatttcccgg ctgacgacag agcatgccat gtatcatttt atttccggtt
     1441 ataccgcaaa agtggccgga acggaaatgg gcatcaccga accgcagacg actttctccg
     1501 cctgcttcgg caaagcgttc ctcccgcttc acccgggaaa atacgcggag cttctcggta
     1561 aaaaactgaa agccgacaac agcatacgcg tgtggcttgt caacacggga tggaccggcg
     1621 gcgcctacgg caccggaagc aggatcaagc tttcctacac ccgcgcgctg atcaccgctg
     1681 cgctggacgg ttcgctcgac aaggcggatt tcaaaaccct gcctgttttc aacctcgcgt
     1741 atcccactac ctgtcccggc gttccatccg aaatactgaa cccgcgcgac acgtggcacg
     1801 acaagagcgc gtacgatgca aatgccgcga agctggccgg tcaattcgtt cagaacttcg
     1861 aacagtacgc ctccggcgtt acgcgggaga tcctcgaggc tgcaccgcag gtggcggtca
     1921 gcgcgtaatt gccgcaaccg ttttcaaccg aaaaactccg tggttctccg cggagttttt
     1981 ttctgaccgc cggtttgcta attttcattc gcaaaaaatg aagaaacggg tacagctttt
     2041 tttcgaagca ggccatcaca attcacggtc ggtatggccc gtcctcttcg gcgtgatcct
     2101 cgggctcacc tgcctgcgcc agcacctcgt ctccaattac aactgcgagc tctgcgccga
     2161 caaagccggg tattacatgt acctgccggc ttctttcgga atgggatttc atgcggatgc
     2221 ttatcccggg aatttcgatc acgatcacgg cgacggtttt catctcgacc ggcagagcaa
     2281 ccgcgtggtg acaaagttca cctgcggagt cgcgatcctc gatgcgccgt tctacgctct
     2341 cggtgcgatc gcagggcgca ttttttcgcc gggcacttcg ccgtattcct atttctatct
     2401 tttcttcatc aatatcggcg cagcatttta tgtaacggcg ggaatgtatt ttctgcgcag
     2461 gtggcttgat cattacaccg ggcggatcac ttcgttcctg gccgtgctcg cggttttcgc
     2521 cgggaccaac ctttattact acacgcttga tgaaagcctg atgagccatg tctattcctt
     2581 cgcgctgttc tgcttcgtgc tgtacgccat gaaagaatat acgctgagcc attcgttcag
     2641 tcatttcatt ttcttttccg tttcgctttc gctggcgata ctgatacgtc ccaccaattt
     2701 cctgttcgga atttttgcat tgctgacgga tgtgaaaaac cccggcgacc tgaagcggaa
     2761 attttctctt tttatttctt tccggaatat caccgctttt ctcgtcatct tctttctcgt
     2821 catgctcccg caggtcattt actggaaatt cgcattcggg aaatacgttg tttggtcgta
     2881 cgagggagaa ggattcacca actggaacga cccggcattc cttacggtat ggttctcgcc
     2941 gcagagcggg cttttcacgt atacgcccct gctgctcgtc ccgctggtct tcgcggtgta
     3001 catggtgcga aaaaaaatac cgaacggcgt tcttgtcctc gctgcatttt tcatcgtcag
     3061 ctatttatgc gcgagctggc acaatccttt tttcggcgtc tgcaatttcg ggaagcgtcc
     3121 tttcgtggaa tacctgccgc tgctgatgtt ccctcttgca ctgcttttcg aacgcttcgg
     3181 ttcattgccg aaattcaggc gcaacatcgt cctcgtttcc gtcctgttct tcatctatta
     3241 caaccaggcg ttgttcgcgg cgttcaacac ctgctttccc ggcagcgaat gggactggcg
     3301 cgaattcttt catttcctcg gccgggcact tccgctgatc cggtgatttc cgctaatttg
     3361 cgtttcgcaa ctccaccgcc tgtgtgtatg aactattttc cggctatctt ttgtttcatc
     3421 accagccttt tctttttttc atgcagcact tcttccccgc cgggaataaa aaacacagct
     3481 gatacggtag ttgttcctgc ttcaaaaaac aaaaacactc ctgcctccgc acaggacaca
     3541 tcatccgtgc tcgacgtgca taagctttgc cagctggccg attcgttttg gacgaaaact
     3601 tcgggacgga gcgtacatct ttccctgcag catgacacgg gaagcagcgt gcagttctac
     3661 cgcacggata tgctggaggc cgtcaacccg gcaggaaatg cgaagcagga aaaatttttc
     3721 gtgtttgaac ccggggggaa caaggacttt tccttttccg tcatcacgct cacctatccg
     3781 ttcatcatcg acgcgatgaa tgcattcagc cgcctgaaaa aaattaccgg catgaacaaa
     3841 gcgttcggcg acatgacgcc gggacttacc tacgagaacg attacgtgct gctttcgggg
     3901 aaaacgattt tctggatgaa tgcgtcctgc gcgtatgaca ggaaaagtac gggagcgatc
     3961 agcgcctttg ttcttcattc gctgaaaacg gaagagccgg tcgactcgat acgctgcggt
     4021 tgcgggcagg cgcaatgcga tactttgtaa cagatgaagc acgtccacca ggctgtccgt
     4081 tttatctgct gcgcaactct gctttccttt attacatctt gtggaacgca ggcaggagaa
     4141 aaagaacctc gtccgggcgg gaggaaaaac gattcctcca ccggactgcc ggctgactat
     4201 aaaaactatg taccggccgc gaacggtttt taccgcggga aagacggggc tttttacatt
     4261 cttacacggg ccgacgatat ccggggagaa cattacgata cacggcccta tttccggaaa
     4321 gtgaatgaag tggatccggc gacgttccac gacattgatg cgtatggcat ttacgcagaa
     4381 gacagctttc acgttttcga acatttccac atggctgaag gcgatcacat tttcctgctc
     4441 gacagcgccg atcgcaactc gtttacggtt atgggctacc ggaacgccaa agacaaacac
     4501 cacgtttatt ccgacaacga ggcgctcgac ctggatccgt cctcgctggt gatcgtcgat
     4561 gccgattcgc tgccggacgg tcgtgtctac atcggttgca tgaaggacaa acagcgtgtt
     4621 tattttgagg gcgaagagca aggcaatgtg gatgcgccat ctttccatct ttgctcgcgc
     4681 aggcacgata cagttatttt ttgcgataaa taccgcgatt atcgcagctg cgatcaatgc
     4741 cagggcctgg aaaacatgaa atgatcgcag ctgacagcgc aacgaactgc acagacccgg
     4801 cggcaatgac gcgaagcctt atctttgccg catggaactg atcattgccg agcaaatcac
     4861 gaagtcgtac ggatcgctgc aggtgctgaa aggaatcgac ctttcgatac ggaagagcga
     4921 agtggtttcc atcgtcggcg catccggtgc gggaaagacg acgctgctca cgatcctggg
     4981 aacgctcgac aaacccgacg gcggaaggct gatgatcgac gggcaggaac tgtccgggat
     5041 gaaccagaaa gccgcggcga tcttccggaa caaaagcatc ggtttcgttt tccagttcca
     5101 ccaccttctc ccggaattca ccgcgctgga aaatgtgtgc atccccgcgt tcatcgccaa
     5161 aacaccaaga aagcaggcgg aagaaaacgc gaagcagctg ctggatatgt tcggtctcgg
     5221 cgaccggctg aagcacaagc cttcggaact ttccggtgga gagcagcagc gggtggccgt
     5281 tgcgcgcgcg atgatcaaca aaccttccgt cattttcgcg gacgaacctt cgggcaacct
     5341 cgattcggtc aattcaaaac acctgcacga attgtttttc cggctgcgga aagatctcgg
     5401 gcagacattc gtcatcgtca cgcacaacga agagctggct tccatggccg accgtaagct
     5461 gacgatcaaa gacgggaaag tcctgtgatc tttcagaatt aataacgcag gatgaagaag
     5521 aaaaaattcg tcctgcgctg tgtcattaag gacaggggcg gcatcctttc ggagccgccc
     5581 ctgttgtcat aaagcgcaga cctgtaagcc gggttctgtc cccttcagcg aacgaatcgc
     5641 ttccggtgcc tgtcatttct cttggcgcaa cgtcgccgtt acgctccagc aacctacccg
     5701 ttccgaccgg cgaagtttcc cccgccatgc gacgggccgc cgcagtttcg gaacttattt
     5761 ggtttttcaa cgcccggggt ttaccccgcg taactgtcgc cagctacggc gtgcgctctt
     5821 accgcacgtt ttcaccctta ccacgcccgg aggcgcggcg gttttgtttc tgtggcactt
     5881 tccatcgccc gcattgctgc cggcgtcttc ccgttaggaa gcggaccacc ctgtgttgcc
     5941 cggactttcc tccatcatcc gccggaattc ccgtgaaggc gaaccttcaa aaaatcccat
     6001 tgaacaacag cgacaggcca gtctgcagga acaaagttac gaaaacaaag ggggctcaga
     6061 atatttcggc ttccgtggcg ccgaaaccgt accgctggaa agaggcgtcg tggaagcgga
     6121 cgtttttata cgtcgacagg atcttcctta cttcctgctt cagtctccca ttaccgacgc
     6181 catgaatgaa aatgatgcgc tgcaggtgcg cggctatcgc agcatcgagc tctttctgca
     6241 ttttgcgaag ctggacatcg atgagctgtg cgttcgtcat gcccgcccag ttgtcgagta
     6301 gctcctcaat gtggaggtct acctcttttt ccagcacctg gtttttcagg tggggcaccg
     6361 acgtcttcgt gcctttgaaa ttcttttctt tttcaagcat catcctgcgg atgtcctcgt
     6421 tcgtcagctt cggtgcatct tcttcttccg cgcgttcatc gtcaaaaaga aaaacttccg
     6481 tcacccacga cttcttcccg gtgagcgcat gttcgttgta aatgctgtcg cggaaaaatt
     6541 tcacgccttt gaatttcgtt acgtggctga ccggcgggac atgcacataa tcgtcgccac
     6601 tgaaaaaaag cacgtcagtc tttacctgtc cccattcatc cgcctgctgc ggtgtcagcg
     6661 tatcgactgc gcgttcagcg tgcgggcgca gcgcaccgga ttcaacgcag atccacgagt
     6721 ttccgttttt gacagagatg gtgtagtaaa catcgtaatc ggaatgattg atgatgcgga
     6781 attccagttc ggatgctgac gggaaatgct ggttgagcgg cacgaatgcg aggaagatgc
     6841 cgtcggggaa gcgcgtattg gcgggaacgg aaacggagag gttcttttcc gcaggcgcag
     6901 tttcttcctg tttttttgcg ggctctgtcc tcacaggttc agccgggacg agctgggctg
     6961 cagggtagtt gtattcgaat ccttccgtg
//
LOCUS       DASTSF010000021        13125 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1765827,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000021 DASTSF010000000
VERSION     DASTSF010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 13125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13125
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1765827"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="VFU15_00615"
     CDS             complement(<1..194)
                     /locus_tag="VFU15_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013454458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HEU4716298.1"
                     /translation="MKTNMGNADRIIRVVIAALFAVLFFTGTVTGGAGIALLVVAGVF
                     MLTSIVGFCPLYLPFGISTC"
     gene            complement(416..1474)
                     /locus_tag="VFU15_00620"
     CDS             complement(416..1474)
                     /locus_tag="VFU15_00620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716299.1"
                     /translation="MKLRHLIPVLILCAGIQVNAFADDTEDSLGLPGDNLDLYGVLDL
                     YKQAASMEDFEKALNNPDNQVNNLDLNNDGNVDYLRVVDNVDGDAHAIVIQDPVSDNE
                     SQDVAAIEIEKKGDGVANLQIVGDEELYGTNYIVEPSEDNNLSSPSDAKFSNFRPTVV
                     IVNVWAWPCVRFIYAPAYVVWISPWRWAYYPVWYHPWHPVAWRVHHGWVVRYHHPYYH
                     CVHECRMTHAHQVYYGHRTTSAAVHRQYQQMHAQNAGRSAGRANAASSAKTSSAPRDH
                     GSGNPSGKTSSPNHSPQSKGGQGGHSSQGHGTGKTGGPQNKGQHGGHSASGHAGGGHQ
                     GGGPKGGKGGGGGRHGGR"
     gene            complement(1591..1884)
                     /gene="gatC"
                     /locus_tag="VFU15_00625"
     CDS             complement(1591..1884)
                     /gene="gatC"
                     /locus_tag="VFU15_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016194398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEU4716300.1"
                     /translation="MKVDSKTINDIAHLARLEFSREDEAKMISDMNRMIGFVEKLNEL
                     DTQNTEPLIYMTDEVNHLREDVAKEPLPQKEVLMNAPKHDSDYFKVPKVVEKK"
     gene            complement(1881..2645)
                     /locus_tag="VFU15_00630"
     CDS             complement(1881..2645)
                     /locus_tag="VFU15_00630"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEU4716301.1"
                     /translation="MRHVFGMIPRALWKIFFILNFVVGLVLLYPFFFFLISVRKNYGA
                     AFRLMRFHARWVLHVPGIFLRVRAEIPLEELPGRCVFVANHSSYLDIVISYCVIPKFF
                     VYMGKAEIDKAPLLRIFFREEKDGHSGMNIYVDRKTRTGSYAAFQEAGMKLRQGQSVF
                     LYPEGTIEPRGNLKPFKNGAFRLAIENQVPVVPVTFRNNWKLLQNGGFLKAYGRPGIA
                     GVIIHKPVPTAGLTEEDLVNLRHSVRDIIARALKEK"
     gene            2761..3732
                     /gene="trpS"
                     /locus_tag="VFU15_00635"
     CDS             2761..3732
                     /gene="trpS"
                     /locus_tag="VFU15_00635"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009055165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEU4716302.1"
                     /translation="MARILTGIQSSNIPHLGNLLGAILPAIKLSKEGGNSGIYFIADL
                     HSLTTIKDAQFRRESSHAVAATWLAFGFDTDKNILFRQSDVPEVCELTWYLSCFMSYH
                     RLQLAHSFKDKADNLSDVNAGLFTYPVLMAADILLYDTNIVPVGKDQKQHVEFTRDIA
                     EKINHHYQKDIFVVPDVQIDEQVMVVPGTDGRKMSKSYNNFINIFLPEKELKKVVMSI
                     VTDSTPLEAPKNPDTDNVFALYKLLGTNEQTEELRKKYLAGNFGYGHAKTALLELILD
                     RFRNEREAYAHFMNDKGALDAELKKGEDKARTIATPVLARARDVFGF"
     gene            complement(4177..4539)
                     /locus_tag="VFU15_00640"
     CDS             complement(4177..4539)
                     /locus_tag="VFU15_00640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716303.1"
                     /translation="MKRLVYFFPLFCVLLSSCRKQDTTVKIAYDIKELSSDNPSYTIT
                     YTSDKSGNSKQVSTSDPLWNSGYIELPKGEYVSLRVDCSAPLYEFRISIYQEGVSVQE
                     TELFNPRGSASVSFTTGN"
     gene            complement(4646..6016)
                     /locus_tag="VFU15_00645"
     CDS             complement(4646..6016)
                     /locus_tag="VFU15_00645"
                     /inference="COORDINATES: protein motif:HMM:NF016994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin esterase family protein"
                     /protein_id="HEU4716304.1"
                     /translation="MKKGFPFILAAFFLLSFTPTVTLSHDETLLKKYCTFSRYTQFMN
                     ADSFSSVYPPHIFDNRRVIACGESSHGSHENQLIKTAMFRYLAEKENVRVFILEASYA
                     EVMTVNDYIHGKEIPPDSAMHSTSFWVWRTQEIRDLIEWMRNFNKGKTAAQQLYFLGM
                     DMQEIRSAAKRSLEFFTLYDPSGSSKAKTLLQPFTTDTFYRIMRFPAEKKQQRMKNLA
                     SVDSLAALVPVPADPAAKEDFLLALHEISVLRQSFQFSAFLGPRGFVERDSAMAENVK
                     WIAGNISPGHPVFVCAHNEHVAVKRYNFMKKYPYASTGALLRDFFGDAYYSIGVDFTT
                     GSFNALTFPGRKSLKNLRDIATGKAMFEGPMPCNFEKKFRHTLFFHVAKYNSWMTWVN
                     CDAAKNDPALMKSKLLRKENVIHSIGAGFLWESACLAATEFGKEFDSFLFFKRSTPSW
                     ILHDPL"
     gene            complement(6040..7026)
                     /locus_tag="VFU15_00650"
     CDS             complement(6040..7026)
                     /locus_tag="VFU15_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018278315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl transferase"
                     /protein_id="HEU4716305.1"
                     /translation="MTGHRLQDHIFTVDETSFTALALEIFRFQLAHNPVYAAFSKGLG
                     KNRDNVNSTGEIPFLPVELFKSHRIYSAEKDPQIVFTSSGTTGSVTSRHEVADVSLYE
                     KSFLAGFEKFYGPPGEWTILALLPSYLEREGSSLVYMADRLIRESKSEDSGFYLHNYK
                     ELSGKLSLLNRQKKKTLLLGVTYALLDLAEQFPQPLENITIMETGGMKGKRKEMVREE
                     VHAILEESFHVKNIHSEYGMTELLSQAYSSGGGIFRTPPWMKILIREMNDPLSFCGSG
                     RTGGVNIIDLANLYSCSFIATSDLGKIHGNNSFEILGRFDFSDVRGCNLMVE"
     gene            complement(7055..8125)
                     /locus_tag="VFU15_00655"
     CDS             complement(7055..8125)
                     /locus_tag="VFU15_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HEU4716306.1"
                     /translation="MLLLIFITIFIALIFDFVNGMNDAANSIATIVSTRILSPRIAVL
                     WAAFFNFVALFIFGTKVAGTVGSGIVHTEILQDGKGVYMIMSALLGAIIWAYFFCARF
                     GIPVSSSHSLIGGLMGAAVIKGGFGSLVLGSGAGFFGTKIFITLLFIVCAPVIGLIIG
                     YFIMVITMWIAKGFHPAKTDRVFRVGQLMSSAAFSIGHGGNDAQKTMGIIAMLLYSAK
                     DVPFVHEYLYTFSNLEKGKFPVPHWVEILALSVIALGTFSGGMKIVKTMGMKLTHLKP
                     IGGFSAEVGGAATLFTATAMGIPVSTTQTIAGSIMGVGTTRRFSAVRWGVAGNIILTW
                     ILTIPMSAVLGALVYWVMSFFI"
     gene            complement(8135..8782)
                     /locus_tag="VFU15_00660"
     CDS             complement(8135..8782)
                     /locus_tag="VFU15_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014681007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HEU4716307.1"
                     /translation="MRLDGFLQFLVPKDKKFFPLFEQASENLVKTSNVLLELVSTNDV
                     QKRKELIREVEHLEHLGDNITHLIFNELGTNFITPFDREDIHALASAIDDVLDFIHGT
                     AKRIDLYRIEEMTEPMIKLSELVLKGSVELNVAVRQLRNLKDINIIKEACVKVNSFEN
                     IADEVFNNGIARLFEEEQDAVKIIKIKEILSALETATDKCEDAANVIQSIIIKTT"
     gene            complement(8951..9634)
                     /gene="phoU"
                     /locus_tag="VFU15_00665"
     CDS             complement(8951..9634)
                     /gene="phoU"
                     /locus_tag="VFU15_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009132205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEU4716308.1"
                     /translation="MWPDPTAMSHLDTEIHLLKEELIEMHRMVRSQLSKCKTALVKFD
                     TDMAKEVLVNERRVNALELKIDRDCENILALLNPVAVDLRFILAALKINSNMERIGDN
                     AEGIARYVLDVTTPFDQQMLADYEVEKMYQVAVSMIDDALEALITEDTSLARKVFLKD
                     DILDSINSKATGITLRYIRENRGDSLHFLNVLSIIRKLERVGDQTKNVAEEIIFYIEA
                     KVLKHGSKI"
     gene            complement(9712..10545)
                     /gene="pstB"
                     /locus_tag="VFU15_00670"
     CDS             complement(9712..10545)
                     /gene="pstB"
                     /locus_tag="VFU15_00670"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HEU4716309.1"
                     /translation="MEGTILNKDTQGVSAAQDRTTGKPAGNDVILKAENFDAYFGANQ
                     VLKKVNVEFFRNKVTAVMGPSGCGKTTFIRCINRMHELTPETKVDGQLMLDGEEIHRM
                     NPIVLRRKVGMVFQRPNPFPTMSIYENVIAGYILNGIRLPRQEKDRIVEESLTRAALW
                     NEVKDNLHKKGTFLSGGQQQRLCIARAIAMKPEVLLLDEATSALDPISTAKIEELIWD
                     LREKYTIITVTHNMQQAGRVSEHTAFFYLGELIEFGLTKEIFTNPKNPKTQNYITGRF
                     G"
     gene            complement(10527..11372)
                     /gene="pstA"
                     /locus_tag="VFU15_00675"
     CDS             complement(10527..11372)
                     /gene="pstA"
                     /locus_tag="VFU15_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEU4716310.1"
                     /translation="MDKALRFRNFKSLFFKGLVIALTALATVPLLLILFYIFRQGISA
                     ISWSFLTDLPKPVGETGGGVFNALIGTLLIILIAAILAIPFGISIAVYLKENPKTKIA
                     YWTSLCVDVLQGIPSIVIGIVIYLWVVKPMGGFSAVSGSIALAFMMLPSIIRSAEETL
                     KLIPESLKEASLALGVPYYRTMLRVILPAGLSGIMNGVILGVARIAGETAPLLFTAFG
                     NPFTNYNLMKPMETLPHIIFNYATSPYEDWHRLAWGASFVLISFILVLNILTKIAERK
                     WKVQF"
     gene            complement(11365..12267)
                     /gene="pstC"
                     /locus_tag="VFU15_00680"
     CDS             complement(11365..12267)
                     /gene="pstC"
                     /locus_tag="VFU15_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEU4716311.1"
                     /translation="MELLSLKSTNRKIWLERVFRRTLLSQAIFFVVLLLAIGVTLVMH
                     SLPSIKALGFGFVYGKDWDPVSDEYGALPFLIGTLITSFLALLISVPFSLAIGLFLGE
                     YFPKGPVSAVVKNMFELLAGIPSVIYGFWGIFVLVPIMRDIEMKAHVPPVGVGILTAS
                     LILSIMVMPYSASLIRQVITMVPSNLKEAAYSLGATKFEVIRKVVLPNIRSGLFAGVL
                     LSLGRALGETMAVTMLIGNTHIIPTGIFSTGNTMASVIANEFTEATGDVYFSTLIEMG
                     LLLFIVTTIINMIGRRIIKRFSNG"
     gene            complement(12363..>13125)
                     /gene="pstS"
                     /locus_tag="VFU15_00685"
     CDS             complement(12363..>13125)
                     /gene="pstS"
                     /locus_tag="VFU15_00685"
                     /inference="COORDINATES: protein motif:HMM:TIGR00975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HEU4716312.1"
                     /translation="PVMHIPTCLGAVIVTYNLPDNPKLKMAPDVVADIFLGKITKWND
                     ARIKADNPDAKLPSMDITVVHRSDGSGTTYVFSDYLSKVSPSWATKPGKGKSLDWPVG
                     IGAKGNEGVGGMVKQTPGSIGYVELAYVLQNQMTGASIRNKSGNFVDANLNSISAAAN
                     VTMPDDFRVSLTDTDAPDGYPISSFTYLLVYTNQSYDNRSQAQAKGTIDLITWMIHDG
                     QQFASPLQYAPLPASVVTSVDAAMKKITFDNKPLL"
BASE COUNT         2982 a         3335 c         3650 g         3158 t
ORIGIN      
        1 ctgcacgtgg aaataccgaa gggcaggtac agcggacaaa atccgacgat ggaggtaagc
       61 ataaacacgc cggcgacaac cagcagcgca atcccggcgc ccccggtaac tgttcccgta
      121 aagaacagca cagcaaacaa cgcagcgatc accacacgga tgatccggtc tgcatttccc
      181 atattcgttt tcatagtaaa gtttttaaag gtggacaacc cggataacga tcaccgagat
      241 catgatgact gttgcactcg ctgcgcacat caccataaat tttatgatct gtattccttt
      301 catgacccca aaattattcc ggggtgtgct ggtacgaagt gatgcaggtc acagaaaaga
      361 gtgagagatc acaaaaaaac cgggaagatc ccggttctga aatttgaatt tgtgctcagc
      421 gtccgccgtg ccttccgccg ccaccgcctt tgccgccctt cgggccaccg ccctgatgac
      481 cgccgcccgc atgcccggat gcggagtgtc cgccgtgctg gcctttgttt tgcggaccgc
      541 cggtttttcc cgtgccgtgg ccctgcgaag aatgtccgcc ctgtccgcct ttactttgcg
      601 gactgtgatt cgggctgctg gttttcccgc tgggattccc cgatccgtga tcgcgcggtg
      661 cagaggatgt ttttgcgctg gaagcggcat ttgcacggcc tgcgcttctg cctgcatttt
      721 gcgcatgcat ctgctggtac tggcgatgaa ccgcggccga agtcgtgcgg tgaccatagt
      781 aaacctggtg cgcgtgcgtc atgcggcatt catgcacgca gtgataatac gggtggtgat
      841 aacgcactac ccacccgtgg tggacgcgcc atgccaccgg gtgccacggg tggtaccaca
      901 cgggataata cgcccatctc catggcgaga tccagacaac atacgccggc gcataaatga
      961 aacgtacaca gggccatgcc cacacgttca cgatgaccac tgtcggccgg aagttgctga
     1021 atttcgcgtc ggaaggggaa gaaagattat tgtcttccga aggctcaacg atataattgg
     1081 tgccgtataa ttcttcatca cccacgatct gcaggttggc cacaccgtcg ccttttttct
     1141 cgatctcgat cgccgctacg tcctgcgatt cgttatcact cacagggtcc tggataacga
     1201 tcgcgtgcgc atccccgtcg acattgtcca ctacgcggag ataatccaca ttgccgtcgt
     1261 tgttgaggtc gagattattg acctggttgt cgggattgtt gagcgctttt tcgaaatctt
     1321 ccatgctggc ggcctgcttg tacagatcga gcacaccgta caggtcgagg ttgtcgccgg
     1381 gaagaccgag ggaatcttct gtgtcgtcag cgaacgcgtt cacctgtatg cctgcacaca
     1441 ggatgagaac gggaattaaa tgacgaagtt tcatggtatt cagtttttga tttgacgcat
     1501 cctgcgcttc atggtttagt tcagccgggg agagaattca aaaaacatgc ccttatcaat
     1561 aaaaaagacc gctccgggcg gtctgctttg ttattttttc tcaacgactt tcggcacttt
     1621 gaaataatcc gaatcgtgtt tgggggcgtt catcagcact tctttttgcg gaagcggctc
     1681 ttttgccaca tcctcgcgca ggtggttcac ttcatcggtc atgtaaatga gcggctccgt
     1741 gttctgcgta tcgagctcgt tgagcttttc aacgaagccg atcatgcggt tcatgtcgct
     1801 gatcattttc gcttcatcct cccggctgaa ttcgaggcgg gcgaggtgcg caatgtcgtt
     1861 gatcgttttg ctgtcgactt tcatttttct ttcagcgcgc gggcgataat gtcgcgtacg
     1921 ctgtgacgca aatttaccaa atcttcctca gtgagacctg ctgtcggaac aggtttgtgg
     1981 atgatcacgc cggcgatgcc ggggcggccg taggctttga ggaaaccgcc gttctgcagc
     2041 agcttccagt tgttgcggaa ggttacaggg acaacgggca cctggttttc gatggcgagc
     2101 ctgaacgcgc cgttcttaaa tggtttcagg ttgccgcggg gctcgatcgt tccctcgggg
     2161 tagagaaaaa cgctctggcc ctgccgcagc ttcattcctg cttcctggaa tgcagcatac
     2221 gatccggtcc gcgttttccg gtcgacataa atgttcatgc cggaatgccc gtccttttct
     2281 tcccggaaaa aaatgcgcag caacggcgct ttgtcgatct ccgcctttcc catgtacacg
     2341 aagaatttgg gaatgacgca atagctgatg acgatgtcga ggtaagacga gtgattggcg
     2401 acgaacacgc agcggccggg caactcctcc agcggaattt ccgcccggac gcgcaggaag
     2461 ataccgggaa cgtgcagcac ccatcgtgca tggaaacgca tcaggcggaa agccgccccg
     2521 taatttttcc gcacggaaat aagaaagaaa aagaagggat acagcagcac gagaccaaca
     2581 acgaaattca ggatgaagaa tattttccac aatgcacggg ggatcatccc gaagacatgc
     2641 cgcattgagc aaaggtaagg ctatcgcgat taagaacgtt tatcgcgccg ggcggcgggg
     2701 cataattccc atcctccgta tattcgtgtc ctttccttca acaatcaatt ttcattcggc
     2761 atggcacgaa tccttaccgg catccagagc agcaatattc cccatctcgg gaatcttctc
     2821 ggcgccatcc tgcccgcgat aaagctttca aaggaaggcg gcaacagcgg catttacttc
     2881 atcgcggatc ttcattcgct caccaccata aaagacgcgc agttccgcag ggaaagctcg
     2941 cacgcggtgg cggcgacatg gctcgcgttc gggttcgata cggacaaaaa catcctcttc
     3001 cgccagagcg acgtgcccga agtgtgcgag cttacgtggt acctgagctg cttcatgtcg
     3061 tatcatcgcc tgcaactggc gcattcgttc aaagacaagg cggacaatct cagcgacgtg
     3121 aacgcgggac ttttcactta ccctgtgctc atggcggcgg atatcctgct gtacgatacg
     3181 aacatcgttc ccgtgggaaa agaccagaag cagcacgtgg aattcacgcg tgacattgcg
     3241 gagaagatca accatcatta ccagaaggat attttcgttg tgcccgatgt gcagatcgac
     3301 gagcaggtga tggtggtgcc gggtacggac ggaagaaaga tgagcaaatc gtacaacaac
     3361 ttcatcaata ttttccttcc tgaaaaagag ctgaagaaag tggtgatgag catcgtgacg
     3421 gattcaacac cgctcgaggc gccgaaaaac ccggatacgg acaatgtgtt cgcgctgtat
     3481 aaactgctgg gaacaaatga acagacggaa gagctccgga aaaaatacct cgcaggaaat
     3541 ttcggctacg gccacgcaaa aacggcgttg cttgagctga tcctcgaccg gttcaggaac
     3601 gagcgggaag cttacgcgca ttttatgaac gacaaaggcg cgctcgatgc agagctgaaa
     3661 aaaggcgagg acaaggcgcg aacgatcgcg acgccagtgc tggcgcgtgc gcgtgatgtg
     3721 ttcgggtttt gagggacgcc ccgcttaagg ccgggccagg tgccgtaacg tttttccgtt
     3781 tgccgacggt tgtttccctt tgtgcacttt gtgaaaagct tcgtgttctt cgtggtttat
     3841 gactcgtcaa cttttcaacc ttgaaactga attgtcttcg ttttcctccg tgcgctgtgt
     3901 gaaaagcttc gtgttcttcg tggtttttag cttgtcaact tttcaacctt gaaactgaat
     3961 tgtcttcgtt ttcctttgtg cactttgtga aaagcttcgt gttcttcgtg gtttttagct
     4021 tgtcaacttt ttaaccttga aactgaattg tcttcgtttt cctttgtgca ctttgtgaaa
     4081 agcttcgtgt tcttcgtggt ttttgactcg tcaacttttc aactttaaac ttgaattgct
     4141 tcaggttccg ggttcggtgc ctggcaattt ctccgttcag ttgcccgtag taaaactgac
     4201 cgaagcgctg ccgcgcggat tgaacagctc ggtttcctgc acggaaacac cttcctggta
     4261 aatggaaatc cggaattcat acaaaggcgc gctgcagtcg acacgcagtg aaacatattc
     4321 tcctttcggc agctcgatgt aaccggaatt ccacagcgga tccgacgtgc ttacctgttt
     4381 cgaattcccg cttttgtcgg atgtataagt gatcgtgtac gaaggattat cggaggaaag
     4441 ttccttgatg tcgtaggcga tcttcacggt cgtgtcctgc ttcctgcacg aagaaagcag
     4501 cacgcagaac aagggaaaaa agtaaacgag gcgtttcatt tgtataatga tacgaaggaa
     4561 ggaaaagcgt aaccggggaa attctacagg tggcggaaaa caatggattt ccggttgaag
     4621 aaccgcgctg cgtgaaagca ggaggtcaca gggggtcgtg caatatccag gatggcgtcg
     4681 agcgtttgaa gaagaggaac gaatcgaatt ctttcccgaa ttccgtggcg gcaagacagg
     4741 cgctttccca caggaagcct gcgccaatgc tgtggatgac attttctttc cggagcagct
     4801 tgcttttcat cagcgcggga tcgtttttcg ctgcgtcgca attgacccat gtcatccatg
     4861 aattgtactt cgcgacatgg aagaacagcg tgtggcggaa ttttttttca aagttgcacg
     4921 gcatgggtcc ttcgaacatt gcttttcccg tggcaatgtc ccgcaggttt ttcagcgact
     4981 ttcttcccgg aaacgtcagc gcattgaaag agccggtggt aaaatcaaca ccgatgctgt
     5041 agtacgcgtc accgaaaaaa tcgcgcagca gcgcacccgt agaagcgtac gggtattttt
     5101 tcatgaagtt gtatcgcttc acggccacgt gctcgttgtg cgcgcagacg aaaaccgggt
     5161 gcccgggtga aatgttccca gcgatccatt ttacattttc cgccattgcg ctgtcccgct
     5221 cgacgaaacc gcgcgggccc aggaaggcgg agaactgaaa ggactggcga aggacggaga
     5281 tctcgtgaag cgcaagaaga aaatcttctt tcgccgcagg atctgccggt accggaacaa
     5341 gcgcagccag gctgtcgacc gaggcgaggt ttttcatgcg ctgctgtttt ttttctgccg
     5401 gaaagcgcat tatgcggtag aaagtatccg tcgtaaacgg ctgcagcagc gtttttgctt
     5461 ttgagctgcc ggaagggtca taaagcgtaa aaaactccag tgaacgtttg gccgccgatc
     5521 gtatttcctg catatccatg ccgaggaaat aaagctgctg cgcggcagtt tttcctttgt
     5581 tgaagttccg catccactcg atgaggtccc ggatctcctg tgtgcgccac acccagaagc
     5641 tcgtcgaatg catcgcgctg tccggcggaa tttcttttcc atggatgtag tcgttcacgg
     5701 tcatcacctc agcatagctg gcttcgagaa tgaacacgcg tacgttctct ttttccgcga
     5761 ggtaacggaa catcgccgtt ttgatcagct ggttctcgtg cgaaccgtgg gatgattcac
     5821 cgcacgcgat cacacgacgg ttgtcgaaaa tgtgaggggg ataaacggaa gaaaaagaat
     5881 ccgcgttcat gaactgcgtg taacgcgaga aggtgcagta tttcttcagc agcgtttcat
     5941 cgtgcgaaag cgtgaccgtg ggcgtaaatg aaagcaaaaa aaaggcggca aggatgaacg
     6001 gaaacccttt tttcatgaat tgaaattacg aaggatgcat cactccacca tcaggttgca
     6061 gccgcgcaca tcgctgaaat cgaagcggcc gagaatttcg aagctgttgt tcccgtgaat
     6121 tttcccgagg tccgatgtgg cgatgaagct gcagctgtaa agattcgcca ggtcgatgat
     6181 atttacaccg cccgttctgc cgctgccgca aaatgaaagc gggtcattca tttcccggat
     6241 gaggattttc atccacggcg gtgtgcggaa aattccgccg ccggaagaat acgcctgcga
     6301 aagcagctcg gtcatcccgt attccgagtg aatgtttttt acgtgaaagc tttcttcgag
     6361 gatggcgtgt acttcttcgc gcaccatttc ctttcgtttc cctttcattc cgccggtttc
     6421 catgatcgtt atgttttcca gcggttgcgg gaattgttcg gcgagatcga gcagcgcgta
     6481 cgttacgccg agcagcaatg tttttttctt ctgcctgttc agcagtgata attttccgga
     6541 aagctctttg taattgtgca ggtaaaatcc gctgtcttcg cttttgcttt cgcggataag
     6601 acgatcggcc atgtacacga gcgaggagcc ttcccgctcc aggtaagaag gaagcagcgc
     6661 gaggatcgtc cattcgccgg gcggaccgta aaatttttca aaaccggcga gaaagctttt
     6721 ttcgtaaagc gaaacgtccg caacctcgtg acgcgatgtg acgctgccgg tggttccgct
     6781 gctggtaaaa acgatttgcg gatctttttc cgcagaataa atgcggtgtg atttgaagag
     6841 ctcgacaggg agaaacggaa tttccccggt tgaattcacg ttatcccggt ttttgccgag
     6901 gccttttgaa aatgcagcgt agacggggtt gtgcgcgagc tggaaacgga agatctccag
     6961 cgcaagcgcc gtgaaactgg tttcgtcaac agtgaaaatg tggtcctgga gccggtgccc
     7021 ggtcatgccg cgaagttcag aatattcgcg gacttcagat gaaaaacgac atcacccaat
     7081 aaacgagcgc accgaggacg gcagacatgg ggatcgtcag gatccaggtg aggatgatgt
     7141 tcccggcaac gccccagcgt acggcggaaa atcttcgcgt cgttcccacg cccatgatgg
     7201 aacctgcgat cgtctgcgtc gtgctgaccg gaatgcccat cgcggtggcg gtgaacagcg
     7261 tggcggctcc gcccacttcc gcgctgaatc cgccgatcgg tttcaggtgc gtcagcttca
     7321 tgcccatggt cttcacgatc ttcatgccgc ctgaaaatgt ccccagcgcg atcacgctca
     7381 gcgcaagaat ttccacccag tgcggaacgg ggaatttccc tttttcgagg ttgctgaacg
     7441 tataaagata ttcgtgcacg aacggcacgt ccttcgcgct gtacagcagc atcgcgatga
     7501 tgcccatggt tttctgcgcg tcgttgccgc cgtgaccgat gctgaacgca gcggaagaca
     7561 tgagctgccc cacgcggaag acgcggtctg tttttgccgg gtgaaatcct ttcgcgatcc
     7621 acatcgtaat gaccatgatg aaatagccga tgatgagacc gatcaccggc gcgcaaacga
     7681 tgaaaagcag ggtgatgaaa attttcgttc cgaaaaaccc ggcgccggaa ccgagcacga
     7741 gcgaaccgaa cccgcctttg atcactgcgg cgcccatgag tccgccgatg agcgaatgcg
     7801 aggagctgac gggaattcca aagcgtgcac agaaaaaata agcccagatg atggcgccga
     7861 ggagcgccga catgatcatg taaacgcctt tgccgtcctg gagaatttcc gtgtgcacga
     7921 tgccgctgcc gacagttccc gccaccttgg tgccgaaaat gaagagcgcg acgaaattga
     7981 agaaggcggc ccagagaacg gcgatgcgcg gcgaaaggat gcgcgtggaa acgatcgtgg
     8041 cgatggagtt cgccgcatcg ttcatgccgt tgacgaaatc aaagatcagc gcaatgaaaa
     8101 tggtgatgaa aataaggagc agcatacgtg cgcgtcaggt ggttttgatg atgatggact
     8161 ggatgacgtt ggctgcgtcc tcgcatttgt cggtcgctgt ttccagggcg gaaaggatct
     8221 ctttgatctt gatgatcttc accgcatctt gttcttcttc gaagagacgc gcgatcccgt
     8281 tattgaacac ttcgtccgcg atattttcaa agctgttcac cttcacgcag gcttccttga
     8341 tgatgttgat gtctttcagg ttgcgcagct ggcgcacggc cacgttgagt tctacagaac
     8401 ctttcaggac cagctcggaa agcttgatca tcggctccgt catctcttcg atacggtaga
     8461 ggtcgatgcg cttcgccgtg ccgtgaatga aatcaagcac gtcgtcgatt gcgctggcca
     8521 gcgcgtggat gtcttcgcgg tcgaacggcg tgatgaaatt cgtgccgagc tcgttgaaga
     8581 tgagatgggt gatgttgtcg cccaggtgct cgaggtgctc cacctcgcgg ataagctctt
     8641 ttcgtttctg cacgtcgttc gtgctgacca gctccagcag cacgttcgat gttttcacga
     8701 ggttctcgct ggcctgttcg aaaagcggga aaaatttctt gtccttgggc acaaggaact
     8761 ggaggaagcc gtctaatctc atgttaattt aatgttacca gtttggttga ccggcggcaa
     8821 atgtaggcca gggtggcgga agccaatgtt aagaaattgt taccagcttc ccggttcggt
     8881 cataaaacat tgaaaaacaa aaagttccgg ttgcggccgg aactttaaaa aggagcagga
     8941 aaatgttttt tcagatcttg gacccgtgct tgagcacttt cgcttcaatg tagaagatga
     9001 tctcttcggc cacgtttttc gtctggtcgc ccacgcgctc cagcttgcgg ataatggaaa
     9061 gcacgttgag gaaatgcagt gaatcgccgc ggttctcccg gatgtaacgc agcgtgatgc
     9121 cggtggcttt gctgttgatg gaatcgagga tgtcgtcttt caggaaaacc ttccgtgcga
     9181 gggaagtgtc ttcggtgatc agcgcttcga gcgcgtcgtc gatcatggat acggccacct
     9241 ggtacatttt ttccacttcg taatcggcga gcatctgctg gtcgaacggc gtggtgacgt
     9301 cgagtacata acgcgcaatg ccctccgcgt tgtcgccgat gcgttccatg ttggagttga
     9361 tcttcagcgc ggcgaggatg aagcgcaggt cgacggccac gggattgagc agggcgagaa
     9421 tgttctcgca gtcgcggtcg atcttcagct cgagtgcgtt cacgcgccgt tcattgacga
     9481 gcacttcctt cgccatgtcc gtgtcgaact tcacgagggc ggttttgcat tttgaaagct
     9541 gtgagcgcac catgcggtgc atttcgatca gttcttcctt gagcaggtgg atttcggtat
     9601 cgaggtgtga catagcggtt ggatcgggcc acatgacccg gagagttttc aaaaaatctg
     9661 cgtcgtaaag gtcggggaaa atattttcca tttgccggtt tcgtaaaacg actacccgaa
     9721 acgtcccgta atgtaattct gtgttttcgg gtttttagga ttcgtgaaaa tctcttttgt
     9781 cagcccgaat tcgatcagct cgccgaggta gaaaaaggcc gtgtgctcgc ttacgcgccc
     9841 ggcctgctgc atgttgtgcg tgacggtgat gatcgtgtat ttttcgcgga ggtcccagat
     9901 cagctcttcg atcttcgcgg tggagatcgg gtcgagcgct gaagtggctt catcgagcag
     9961 gagcacttcc ggtttcatgg cgatggcgcg tgcgatgcag agacgctgct gctgtccgcc
    10021 ggaaaggaac gtccctttct tgtgcaggtt gtcctttact tcgttccaga gcgcggcgcg
    10081 cgtgagcgat tcttccacga tgcggtcttt ttcctggcgc ggaaggcgga tgccgttgag
    10141 gatgtatccc gcgatcacat tttcataaat gctcatcgtc gggaacgggt tcgggcgctg
    10201 gaagaccatt cccactttgc gccgcagcac gatcgggttc atgcggtgga tctcttcccc
    10261 gtcgagcatc agctgcccgt ccactttcgt ttccggtgtc agctcgtgca tacggttgat
    10321 gcagcggatg aaagttgttt tcccgcagcc ggaaggcccc atcaccgcgg taaccttgtt
    10381 gcggaaaaat tcaacgttca cttttttcag cacctggttg gcgccgaaat aggcatcgaa
    10441 attttccgct ttcagaatga cgtcatttcc cgctggtttg ccggtagtgc ggtcctgcgc
    10501 ggcagaaacg ccctgtgtgt ctttattcag aattgtacct tccattttct ttcagcgatt
    10561 ttcgttagga tattcagcac aagtataaat gatatcagca caaaggatgc tccccaggcc
    10621 agccggtgcc agtcttcata cgggctcgtt gcatagttga aaatgatgtg cggcagcgtt
    10681 tccatgggtt tcatgaggtt gtaattcgtg aacgggttgc cgaacgcagt gaaaagcagc
    10741 ggtgcggttt ctcccgcaat gcgcgccacg ccgaggatca cgccgttcat gatgccgctc
    10801 agtcctgcgg gaagaattac gcggagcatg gtgcggtaat acggcacgcc aagcgcaagc
    10861 gaagcttctt tcagcgattc gggaatgagc ttgagcgttt cttcggcaga acggatgatg
    10921 gagggaagca tcatgaacgc aagcgcgatg ctgccggaca cggcggaaaa tccgcccatc
    10981 ggtttcacca cccagagata aatgacgatg ccgatgacga tggaaggaat gccctgcagc
    11041 acgtcgacgc agaggctggt ccagtaagcg atcttcgttt tcgggttttc cttgaggtaa
    11101 accgcgatgc tgatgccgaa cgggatggcg aggatggccg cgatgagaat gatgagcagc
    11161 gtcccgatga gcgcgttgaa cacgccgccg cccgtttcac cgaccggttt cggaagatcc
    11221 gtaaggaagc tccagctgat ggcggaaatt ccctggcgga aaatgtagaa caggatgagc
    11281 aggagcggaa cagtagccag cgcggtcagc gcaatgacga gtcctttgaa gaaaagactt
    11341 ttgaaattac ggaagcgaag cgccttatcc atttgaaaaa cgtttaatga tccttcttcc
    11401 gatcatgttg atgatggtcg ttacgatgaa caggagcaat cccatttcaa tgagcgttga
    11461 aaaataaaca tcgcccgtgg cttcggtgaa ttcgttcgcg atgacactgg ccatcgtatt
    11521 tcccgtgctg aaaataccgg tgggaatgat gtgcgtgttc ccgatgagca tcgttacggc
    11581 catcgtttcg cccagtgcac ggcccagcga aagcagcacg cctgcgaaaa gtcccgagcg
    11641 gatattcgga agaacgactt tccggatcac ttcgaatttc gttgcgccga gcgaataggc
    11701 ggcttccttc aggttggaag gaaccatcgt gatcacctgg cggatcagcg aagcggaata
    11761 aggcatgacc atgatggaaa ggatgagcga tgcggtgaga attcccacgc cgaccggcgg
    11821 cacgtgcgcc ttcatttcga tgtcgcgcat gatgggaacc agcacgaaaa ttccccagaa
    11881 gccgtaaatg actgaaggaa taccggccag cagttcgaac atgttcttca ccaccgcgga
    11941 aacgggacct ttcgggaaat attcaccgag aaaaagcccg atcgcgaggg agaacggtac
    12001 ggaaataagc aacgcgagaa aggaggtgat gagcgttccg ataagaaaag gcagcgcgcc
    12061 gtattcgtcg gaaaccggat cccagtcctt gccataaaca aaaccgaatc ccagcgcttt
    12121 gatggaaggc agggaatgca tgaccagcgt gacgccgatc gcgagcagca aaacgacgaa
    12181 aaagatcgcc tgcgaaagaa gcgttctccg gaagacccgc tcgagccata ttttcctgtt
    12241 cgtgcttttt aaagaaagta attccatccg tgtattttaa cgaccaaagc gagccgccgg
    12301 attacggcag ttcgctttga tcaaaacaag taaaaccagg ttaaccaaac attacgctga
    12361 tattacaaca acggtttatt atcaaacgta attttcttca tggctgcgtc taccgaagta
    12421 accacgcttg ccggaagcgg agcgtactgc agcggcgatg cgaattgctg accgtcgtgg
    12481 atcatccagg tgatgaggtc gatcgtgcct tttgcctgcg cctgtgagcg gttgtcgtac
    12541 gactggttcg tgtaaacaag caggtaggtg aagctggaga tcgggtagcc gtcgggtgca
    12601 tccgtatcgg taagcgacac gcggaaatca tcgggcattg tcacgttcgc tgccgctgaa
    12661 atggaattca ggttagcgtc gacgaagttg ccggatttgt tcctgatgct ggcgccagtc
    12721 atctggttct ggagcacata agcgagttct acgtaaccga tgctgccggg cgtctgcttc
    12781 accattccgc caacaccttc gttccctttc gcaccgatgc ccacgggcca gtcgagcgat
    12841 tttcctttgc cgggtttcgt ggcccagctc gggcttactt tgctgaggta atcggagaac
    12901 acgtaagtcg ttccgcttcc gtctgaacgg tgtaccacgg tgatgtccat ggacggaagt
    12961 ttcgcgtcgg gattatccgc tttgatgcgc gcatcgttcc acttggtgat cttgccgagg
    13021 aaaatatcag cgaccacgtc cggcgccatt ttcagcttcg ggttgtcggg aaggttgtac
    13081 gtaacgatca ccgcgccgag gcatgtggga atgtgcatta cggga
//
LOCUS       DASTSF010000022         3771 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_870162,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000022 DASTSF010000000
VERSION     DASTSF010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3771
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_870162"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..956
                     /locus_tag="VFU15_00690"
     CDS             <1..956
                     /locus_tag="VFU15_00690"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007649376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEU4716313.1"
                     /translation="PDEVHCTTINKVCASGMKSVIFGAQSIMLGDNDVVVAGGMENMS
                     MTPYYLEKARYGFKYGHGQVTDGIVKDGLTDVYHNNLMGSSAELCAAKYNITREQQDA
                     FAIESYKRSAAAWEAGKFAEEVVPVTVKTKKGDVVVSKDEEFTNVKFDKIPALKPVFQ
                     KDGTVTAANASTINDGAAALVLMSKEKATELGIKPLAVIRSYADAEQAPEWFTTTPSK
                     AIPRAVEKAGLKMSDISFYEINEAFSVVSLANMQLMKLDPAKVNVNGGAVSLGHPLGC
                     SGARIIVTLLSVLKQNKARYGVAGICNGGGGASAIVIENLQ"
     gene            971..1750
                     /locus_tag="VFU15_00695"
     CDS             971..1750
                     /locus_tag="VFU15_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017495963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEU4716314.1"
                     /translation="MYGVCHLSAVPCRKEASDRSEMVNQLLFGDAFRVVEKRDSWHRI
                     TTVRDRYDCWIDRKQYIEISKNTFDALEHETHPVTSDLFSIITDVGAAVSFPVSLGCT
                     LPFFSNGKCQVDNRVYTFEGDFFSPADKVSRNRIVETAMSFLNAPYLWGGRTPFGLDC
                     SGFTQVVFKANGIYLKRDAYQQAEYGSPYSFVEEAQPGDLAFFDNEEGRITHVGIVLD
                     DNKIIHASGRVRIDHFDHYGIFTPERGGYTHNLRVIKNVAG"
     gene            complement(1878..2168)
                     /locus_tag="VFU15_00700"
     CDS             complement(1878..2168)
                     /locus_tag="VFU15_00700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716315.1"
                     /translation="MKKIASFVFLIALFSSCVKIKNETQVSFYGTLEKQGITTYQYGT
                     HTIVNGNTTYALKSETINLDNYAGRNVTITGSKIKGYPIDGGPDYIDVTSVR"
     gene            complement(2173..2583)
                     /locus_tag="VFU15_00705"
     CDS             complement(2173..2583)
                     /locus_tag="VFU15_00705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716316.1"
                     /translation="MKKALFILILAAGVVSCRKDSSKPFTVQSGVVRALITVQHHGIP
                     IPYARVFVKQGTLIFPGTDTTLYDLRLQADAEGQVIYTGIPDGQQAYTFYGKGVDPNW
                     DSTHVTPVWGYQFLITDTQPLENKDYPVSLPVSE"
     gene            complement(2673..>3771)
                     /locus_tag="VFU15_00710"
     CDS             complement(2673..>3771)
                     /locus_tag="VFU15_00710"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEU4716317.1"
                     /translation="TAAVLIDKINSLSALRIAIDIPSGLFGDGHPEDQRAHIVQAHHT
                     LTFQQPKLSFFFAENEKYVGRFHVLDIGLHPAFIADAETPFHFVTRAFVHPLLPSRPV
                     FSHKGTFGHALLIAGSKGKTGAAVLCARACLRSGAGLVTVKTSAWGMNVLQEAVPEAM
                     LEENEESDVVAGHIRPEKYSAIGMGPGTGTEKQTEGTLKMLIQDYAGPLVLDADALNI
                     ISGNRTILSFLRRETILTPHPGEFDRLTEKHSSGYERMKTQRELSVKRGIFIVLKGAF
                     TSVSCPDGSVYFNSSGNPGMAKGGSGDALTGMITGLLARGMNERDACILGVYLHGLAG
                     DIAASVHSREGMTAGDLIENIGGAWRMLSGE"
BASE COUNT          872 a          991 c         1040 g          868 t
ORIGIN      
        1 ttcccgatga agtgcattgc accaccatca ataaagtctg cgcatccggg atgaagtcag
       61 tgatcttcgg cgcgcaatcc atcatgctcg gcgataacga cgtggtcgtt gccggcggaa
      121 tggaaaacat gtcgatgacg ccgtattacc tggagaaggc gcgttacggt ttcaaatatg
      181 gtcacgggca ggtgacggac gggattgtga aggacggcct gacggatgtt taccacaaca
      241 acctcatggg cagcagcgcg gaactttgcg cggcgaaata caacatcacg cgcgagcagc
      301 aggatgcatt cgcgatcgaa tcgtacaagc gttcggcagc agcgtgggag gctgggaaat
      361 ttgcagagga agtcgtcccg gttaccgtga aaacgaagaa gggtgatgtc gtcgtttcga
      421 aagatgaaga attcacgaac gtgaagttcg acaagatccc cgcgctgaag ccggttttcc
      481 agaaagacgg aacagttacg gctgccaacg cttcaacgat caatgacggc gctgccgcgc
      541 ttgtcctgat gagcaaagag aaagcgaccg agcttggcat aaaaccgctg gcggttatcc
      601 gcagctatgc cgatgcggag caggcgcccg agtggtttac caccacgcct tcgaaagcga
      661 ttccccgcgc cgtggaaaaa gccgggctca aaatgagcga catttctttc tacgagatca
      721 acgaagcttt ttccgttgtt tcactggcga acatgcagct catgaagctc gatcctgcaa
      781 aagtgaacgt caacggaggc gctgtttcgc tcggccatcc gctcggctgt tccggtgcac
      841 gcatcattgt cacgctcctc agcgtgctga agcagaacaa ggcgcgttac ggcgtggccg
      901 gcatctgcaa cggcggcggc ggcgcaagcg cgatcgtcat cgaaaacctg caatgatctt
      961 ctgagtccgc atgtacggcg tctgtcacct gagcgcagtc ccctgcagga aagaagcttc
     1021 cgaccggagc gaaatggtca accagcttct tttcggtgat gcgttccgcg tggtggaaaa
     1081 acgcgacagc tggcaccgca taaccactgt ccgcgatcgg tacgattgct ggatcgaccg
     1141 caagcaatac attgaaattt cgaagaacac tttcgatgcg ctggagcacg aaacgcaccc
     1201 ggtgacatcg gacctgtttt ccattatcac ggatgtcggc gcggcggttt cgtttcccgt
     1261 ttcgctcggc tgcacgcttc cttttttttc caatggaaaa tgccaggtcg acaaccgtgt
     1321 ttacacgttc gaaggcgatt ttttttctcc ggcggacaaa gtttcacgca acaggatcgt
     1381 ggaaacggcg atgagctttc tcaatgcgcc gtatctctgg ggcggacgta caccgttcgg
     1441 tctcgattgt tccgggttca cgcaggtcgt tttcaaagcg aacggcattt acctgaaacg
     1501 tgatgcttac cagcaggcgg aatacggttc cccgtacagt ttcgtggaag aagcgcagcc
     1561 cggggatctc gcatttttcg acaacgagga aggccgcatc acgcacgtcg ggattgtgct
     1621 cgacgataac aagatcatcc acgcttcggg tcgtgtgcgc atcgaccatt tcgatcacta
     1681 cggaattttc acacccgagc gcggtggcta cacgcataac ctgcgcgtga tcaaaaacgt
     1741 tgcagggtaa acggtcccgc cactttccga ttatttagtg attgcttttg aggtgaagat
     1801 gaccggcgta tttgcacgga tgaattttcc ggaacgttat acgccgtaac tggaagtgaa
     1861 accgtaaagg aaaattttca ccggacgctc gtcacgtcga tgtaatccgg tccgccgtcg
     1921 ataggataac ctttgatctt gctcccggtg atcgtcacgt tccggcccgc gtaattgtca
     1981 aggttgatcg tttcgctttt cagcgcataa gttgtatttc cattgacgat cgtatgcgtg
     2041 ccgtattgat aggtagtgat gccctgcttt tcgagcgttc cgtaaaagct gacctgtgtt
     2101 tcatttttga tcttcacgca ggaagaaaaa agtgcaataa ggaaaacgaa ggatgcgatc
     2161 tttttcatgt ggctattcgc ttaccggaag tgaaacggga taatccttgt tctcgagcgg
     2221 ttgcgtatcc gtaatgagga actggtagcc ccacacaggt gttacatgcg tggaatccca
     2281 gttgggatcg acgccttttc cataaaatgt atacgcctgc tggccgtcgg gaatgcccgt
     2341 gtagatgacc tgtccttccg catctgcctg gaggcggaga tcgtaaagtg tcgtgtcggt
     2401 tcccgggaaa atgagcgttc cctgcttcac gaaaacgcgc gcatagggaa tgggtatccc
     2461 gtgatgctgc acggttatca gcgcacgaac aacgccggat tggaccgtga acggtttcga
     2521 tgaatccttt ctgcaggaaa caacgccggc ggcaagaatc aatatgaaaa gggccttttt
     2581 caaacgagta ttaaaattac gcaattatta cgcgcagggc aagcgtttgt gcaagtggat
     2641 acgccggaag aacaggtggt gcgaactgtt cttcattcgc cggacaacat ccgccaggct
     2701 ccgccgatat tttcgatgag atcgccggct gtcatccctt ccctggaatg aacggaagcc
     2761 gcgatgtcac cggcgagccc atgcaggtac acgccgagaa tgcacgcatc ccgttcattc
     2821 attccacgcg caagcagtcc tgtgatcatc cccgtgagcg catccccgct gccgcctttc
     2881 gccatgcccg gatttcccga ggaattaaaa tacacgcttc cgtctggaca ggaaacagag
     2941 gtgaacgcgc ctttcagcac aatgaaaatg ccgcgcttca cggaaagttc ccgttgtgtt
     3001 ttcatgcgtt cataaccgga gctgtgtttt tccgtcagcc ggtcgaattc cccgggatgc
     3061 ggtgtaagga tcgtttcacg gcgaagaaaa gaaaggatcg tcctgttccc ggaaatgatg
     3121 ttcagcgcat cggcgtcgag aacaagcgga ccggcgtaat cctggatcag cattttcagc
     3181 gttccttccg tctgcttttc tgttcccgtt ccgggtccca tcccgatcgc ggaatatttt
     3241 tccgggcgta tgtgtcccgc cacgacatcg ctttcctcgt tctcttcgag catggcttcc
     3301 ggaacggctt cctgcaaaac attcattccc caggcggatg ttttcacggt gacgagtcct
     3361 gctcctgaac gaagacaggc tctcgcacaa agcacagctg cccctgtttt tcctttgctt
     3421 cccgcaataa gcagcgcatg accgaatgtt cctttatgcg aaaaaacagg acgggaggga
     3481 agcagcgggt gaacgaatgc gcgcgttacg aaatggaacg gagtttcggc atctgcaatg
     3541 aacgccgggt gaaggccgat atcgagcaca tggaaccgcc cgacgtactt ttcgttttca
     3601 gcgaagaaaa aagagagctt cggttgctgg aacgtcagcg tatgatgcgc ctgcacaata
     3661 tgcgcgcgct ggtcctccgg gtgtccgtcg ccgaaaagcc cggaaggaat gtcgatcgcg
     3721 atgcgcagcg cggaaagtga attgattttg tcaatgagaa cggctgctgt t
//
LOCUS       DASTSF010000023         3758 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1092799,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000023 DASTSF010000000
VERSION     DASTSF010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3758
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1092799"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1247
                     /locus_tag="VFU15_00715"
     CDS             <1..1247
                     /locus_tag="VFU15_00715"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU4716318.1"
                     /translation="VDWGGVFAQVEYSGKKWSAFLTGSGSMTYYQRLDYFKKRDVVLD
                     DGTVVPQIVGYNEVYYTNGTQSGVAQNNAVITTSGDTTIINNPSGADYKIVNAKGYAW
                     NSDKARTAQTDRKSFPGFTVKTGANYNFTDHLNAFVNIGYLVLAPRFNTVFDNNNKEI
                     PGVKSQVVFATEIGVGVRYPKFAVNFNAYNTDWKNKPPYSSPTVTIASDQYTYDMTGL
                     NTTLKGIEVDFSVKPLETLELMGMVSIGDWTYHSSGVAYLYDANYVLTDTIAYSAKGV
                     HVGDAAQTMFSGSARWEMFRGFYIKPRYTYFANHYANFDPIYLTPVYNSAHQEVGDNR
                     DHESWKIPAYGLLDLFAGYEFSEKVGDDHKIKLAFNLGVTNLLNTFYISDATNGANYD
                     AASVLIYPGLGRRWTAGMRLMF"
     gene            1355..3553
                     /locus_tag="VFU15_00720"
     CDS             1355..3553
                     /locus_tag="VFU15_00720"
                     /inference="COORDINATES: protein motif:HMM:NF016146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HEU4716319.1"
                     /translation="MKKLITALFFFVTAVTIAQTTLPTSWSFTTTSFPTGWTASGNSY
                     YTGSGNTPPACKFDNTGDWVQIFFSGAPGALSYYIAGNSFAGGTFDVQESVNGTTWTT
                     LHSYSDVTLPAATYTQFTDNPSASSHYIRFYYTNKSSGNVGVDDVTLNAAPAGPQQEI
                     NVKYNSNTVLTGGAVWFSSPVSTMTPENFTIENQGTTNALNVSGATITGPDAGDFSIA
                     TSTPFSVNGLSSSTLTVNFTPSSAGTKTAVLTINNDDADESAYVINLYGVGGNYATEP
                     SASATALAFTNVKSYRIKVNWTNASPLPDGYIVLRNDVSPVTDAPADGAGYSVGDQVG
                     SCKVAYIGSGTSFWANYIGANENYYFAVFSYNGPVPYRNYLTSSSLSGSVTSSGSMQP
                     ANYYSTINTAAPTFVDDLTALSNPHTDNYYSNYGPRMVSLFWARDTSNGDKAVTCVYS
                     GANFVYTEPFQWTVMSREHSYCHSWMPTYPSTTGPEYSDYFNLFPVDQNNANALRSNY
                     PLGEVVNVTSTYLGCKYGTDANGHTVFEPRDEQKGDAARAIFYMAMCYNSASQNWGFP
                     NPISASIMYGQDQNVLKKWNYQDPPDAREIAKNDFIDSLQGNRNPFIDSMNYVCYIDF
                     NTMTKINGPLVPCNNTTIGMGENNPNRTQLRLWPNPTDGVFTLYYQTQENEDITIRLV
                     DVSGRIVFEEKNSAAAGANAYALDLTNVSGGAYTLQVQGKNLINTELILQ"
BASE COUNT          954 a         1021 c          965 g          818 t
ORIGIN      
        1 atgtcgactg gggcggcgtg tttgcgcagg tggaatattc cgggaagaaa tggagcgctt
       61 tcctcaccgg cagcggatcg atgacgtact accagcgcct ggattatttc aaaaaacgcg
      121 acgttgtcct cgacgacgga actgtagtgc cgcagatcgt cggctacaac gaagtgtatt
      181 acacgaacgg gacgcagtcg ggcgttgcgc agaacaacgc ggtcatcacg acttccggcg
      241 acacgacgat catcaacaat ccttcgggcg ccgattataa aattgtcaat gcgaaaggat
      301 atgcgtggaa ttcggacaag gcgaggactg cgcagacgga tcgtaaaagc ttcccgggat
      361 tcacagtgaa aaccggtgcg aattacaact ttacggacca cctgaatgcg ttcgttaaca
      421 tcggttacct ggtgctggct ccgcgtttca ataccgtgtt cgacaataac aacaaagaga
      481 ttcccggtgt aaaatcacag gtcgtttttg caacggagat cggtgtagga gtgcgctacc
      541 cgaagtttgc ggtcaatttc aacgcgtaca acacggactg gaaaaacaaa ccgccgtatt
      601 catcaccgac agtaacgatc gcatcggatc agtacacgta cgacatgacc gggctgaaca
      661 cgacgctgaa aggaattgaa gtcgattttt ctgtgaagcc tcttgaaacc ctcgagctga
      721 tgggaatggt ttcgatcggc gactggacct atcattcgag cggcgtggct tacctgtacg
      781 atgccaacta cgtgcttacc gatacgatcg cgtacagcgc gaaaggcgtg cacgtgggcg
      841 atgccgcgca gacgatgttc agcggatctg cacgatggga aatgttccgg ggtttctaca
      901 tcaaaccacg ctatacgtat ttcgccaacc actacgccaa cttcgatccc atttacctga
      961 cgcccgtgta caacagcgcg caccaggaag tgggtgacaa ccgcgatcat gaatcatgga
     1021 agataccggc gtatggcctg ctggatcttt ttgcggggta tgaattcagc gaaaaggtcg
     1081 gcgacgatca caaaattaaa ctcgccttca acctcggcgt tacgaatctt cttaatacct
     1141 tttacatcag tgatgcaacg aacggcgcca attacgacgc tgcttcggtg ctgatctatc
     1201 ccgggctcgg aaggagatgg acggccggga tgcgactgat gttctgaaac tttttccggc
     1261 acggccggct aaaccgaaaa acaaattccc gcgagcctgt gtgcgcggga taaaaaacaa
     1321 gcactcatcg tcacaggcgg atgagaaaag aaaaatgaaa aaactaatta cagcgctgtt
     1381 tttcttcgtg acagcagtga cgatcgcaca gacgaccctg ccgacgtcat ggagctttac
     1441 caccacgtcc tttcccacgg gatggacggc gagcggaaat tcgtattaca ccggctcggg
     1501 caatacaccg cctgcatgca agttcgacaa caccggcgac tgggtgcaga tatttttctc
     1561 cggcgcaccc ggcgcgcttt cgtattacat cgccggtaac agcttcgccg gcggaacgtt
     1621 cgacgtgcag gaatccgtga acggaaccac gtggaccacg ctccattcat acagcgacgt
     1681 gacgcttccc gcagcaacgt acacgcagtt cacggataat ccttctgcgt cgagtcatta
     1741 catccgtttt tattacacca ataaatcttc gggtaatgtc ggtgtggatg atgtaacgct
     1801 gaacgcagca cctgccggac cgcagcagga gatcaacgta aaatacaatt ccaatacggt
     1861 gctcaccggc ggagccgtct ggttctcttc tcccgtgagc acaatgacgc cggagaattt
     1921 tacgatcgaa aaccagggta cgacgaacgc gctcaacgtt tcaggcgcaa cgattacggg
     1981 tccggatgcc ggcgattttt ccatagctac gtcaacacct ttttctgtga acggcctttc
     2041 cagcagcacg cttacggtga atttcacacc ttcatctgcc ggaaccaaaa cagccgtcct
     2101 cacgatcaat aacgatgacg ccgatgaaag cgcttacgtg atcaacctct atggagtagg
     2161 tggaaattat gccaccgagc cttccgcttc ggcaactgcg ctcgcgttta ccaacgtgaa
     2221 atcctaccgc atcaaagtaa actggacaaa tgccagcccg ctgcccgacg gttacatcgt
     2281 gctgcgcaat gacgtttccc cggttacgga cgcccctgcc gacggtgcag gctattctgt
     2341 cggcgaccag gtgggcagct gcaaggtggc gtacatcggc agcggaactt cgttctgggc
     2401 gaattacatc ggcgcaaatg aaaattatta tttcgccgtg ttctcgtaca acggtcccgt
     2461 gccgtaccgc aattacctca cttcatcttc actttccggc tcggtaactt cttcgggatc
     2521 catgcagcct gcgaattatt acagcacgat caataccgcg gctcctacat ttgtcgacga
     2581 ccttaccgcg ctgagcaatc cgcatacgga taattactac agcaattacg gtccccgcat
     2641 ggtttcgctt ttctgggcgc gcgatacgtc gaacggcgat aaagcagtga cctgcgttta
     2701 ttccggtgcg aatttcgttt acacagaacc tttccagtgg acggtgatga gccgcgagca
     2761 cagctattgc cacagctgga tgccgacgta tcccagcaca acaggcccgg aatacagtga
     2821 ttacttcaac ctgttcccgg tcgaccagaa caacgcgaat gcactgcgca gcaactaccc
     2881 gctgggcgag gtggtgaatg tgacatccac gtatctcggc tgcaaatatg gaacggatgc
     2941 gaacgggcac actgttttcg agccccgcga tgagcagaag ggcgatgccg cccgcgcgat
     3001 cttctacatg gccatgtgct acaacagcgc ctcgcagaac tgggggtttc ccaacccgat
     3061 atccgcgtcg atcatgtatg gccaggacca gaacgttttg aaaaaatgga actaccagga
     3121 tccgccggat gcacgcgaga tcgcgaagaa cgatttcatc gattcgctgc agggcaacag
     3181 gaacccgttc atcgacagca tgaattacgt ctgctacatc gacttcaata cgatgacgaa
     3241 gatcaacgga ccgctcgttc cctgtaacaa tacaacgatc ggcatgggtg aaaataatcc
     3301 gaaccgtaca cagcttcgcc tctggccgaa cccgacggac ggcgtattca cactgtatta
     3361 ccagacacag gagaacgaag acatcacgat ccgcctggtc gatgtttcgg gaagaatcgt
     3421 tttcgaagaa aagaattctg ccgccgcagg cgcgaatgcg tatgcgctcg atctcaccaa
     3481 tgtttccggc ggagcttata cgttgcaggt gcaggggaaa aacctgatca atacggagct
     3541 gatcctgcag tgatcggatt tcatgaaaga aagaacgccg cagatcgcgg cgtttttttt
     3601 gatcacgtga taacctaagc cgggatattg cgtaaaatat tataggaaga atggccggat
     3661 caactcggaa acaccttaac cggccgttgc tatttaatcg attgggcttt tttaacagga
     3721 gcggcttctc ctgcacaacc caatgtttac gggccttt
//
LOCUS       DASTSF010000024         7774 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1540004,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000024 DASTSF010000000
VERSION     DASTSF010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 7774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7774
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1540004"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1262)
                     /gene="ftsZ"
                     /locus_tag="VFU15_00725"
     CDS             complement(<1..1262)
                     /gene="ftsZ"
                     /locus_tag="VFU15_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016195990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEU4716320.1"
                     /translation="MTFEFPEQSSIIKVIGVGGGGSNAVNHMFRQGIKGVDFLVCNTD
                     KQALDMSPVPYKIVLGATLTEGRGAGSLPEVGRNAAIENIEDIRRILSNNTKMVFITA
                     GMGGGTGTGAAPIIAGIARELGILTVAIVTVPFSFEGKKRKQQAEEGLEQLKQNCDTL
                     LVICNDKLREMYGNLRLSDAFAHADDVLTIAAKSIAEIISLTQQINVDFADVCTVMRN
                     SGVAIMGSAVTNGENRAVRAVEMALNSPLLNDNNITGARYVLLNIVSGEEEMTMDELG
                     EITDYIQEAAGQTAEIIKGYGVDSSLGDAVSVTIIATGFNQNNPVSYEYSRKPAQQKI
                     VLNLDEAKSVAETPLASVTEEMKEVKAEANPLEPFLLSEIEAKENTAAPVSDSGKDEK
                     KEEITNPLEPFLVVKQEEETKEETLTVVS"
     gene            complement(1799..3073)
                     /gene="ftsA"
                     /locus_tag="VFU15_00730"
     CDS             complement(1799..3073)
                     /gene="ftsA"
                     /locus_tag="VFU15_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015809126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEU4716321.1"
                     /translation="MDNEIVVGLDIGTTKIAALVGRRTDHGKIEILGMGKAESLGVAR
                     GVVLNIDETVKAIRQAVDEASNKSGVDIKVVNVGIAGQHIKSLQHRGMKTRRSIDDEI
                     SQKDIDELIEDMYRLVMSPGEEIIHVIPQEYIVDNEVGIKNPIGMAGTRLEANFHIIT
                     GQVAAAKNIYKCVAKAGLEAADLTLEPLASADAVLSDEEKEAGVVLVDIGGGTTDIAI
                     FHDGIIRHTAVIPFGGNIITEDIKEGCSIIKTQAELLKIKFGSALASENQENEIVSIP
                     GLRGRPHKEISVKNLAHIIQARMEEIIDHVYYEIKNSGFEKKLIAGIVVTGGGAQLKH
                     ITQLMEYITGMDTRVGYPNEHLASSSEEITSPMYATGVGLVMRGLLATQKSKANEEKT
                     NKVQNHSQKTKGGWFDQIFSKGKQWFEEDSGN"
     gene            complement(3063..3878)
                     /locus_tag="VFU15_00735"
     CDS             complement(3063..3878)
                     /locus_tag="VFU15_00735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716322.1"
                     /translation="MKKLIYIGLWIVAFAGAIVTMSFASRSHAARKCGKLYVDISPPG
                     NHFFIRPEDIRELLSAHAMMPEGKSISDIDVNGIEKLLLTHPAVESCDAFISIDGNVT
                     VKIRQRNPVMRFMNYNGESYYMDDKGKLMPWSEEYTAPLILVSGYFGDGYAAMQHIDF
                     SKISADSALKTPTVLDDAWQIVRKLEADSFLTAQITQVFISPDEGIQLIPRIGSQSIV
                     IGDIRDLDDKLYRLRLFYREGLGKTGSWNDYSKIDLQYKNQIVCTKKNNDHGQ"
     gene            complement(3875..5296)
                     /gene="murC"
                     /locus_tag="VFU15_00740"
     CDS             complement(3875..5296)
                     /gene="murC"
                     /locus_tag="VFU15_00740"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012929234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEU4716323.1"
                     /translation="MKLNAIRYVYFLGIGGIGMSALARYFQAAGKKVAGYDRTRTALT
                     DELAAAGMEIHFEDDVKNIPAEYIAGKEKLLVVWTPALPPDHRELNFFRDGGFTLMKR
                     ARVLGIIAENSTTIAVAGTHGKTTTTTLTAHLLRTSGIDSSAFLGGISGNYHTNLLLG
                     KNIAKENVPAGENILVAEADEYDRSFHWLHPEVAIVTSADPDHLDIYGNRNEMDESYR
                     KFISQVKRKLITRRKVLDQLGIPVTEKVLTYSLEEDSSDAFAKEIRVEDGFYHFDLVI
                     RGKKIEGLKLGLPGRHNVENAVAAVTAAKEFGIGDESVKSALASFRGVQRRFDIHLRD
                     PECVYIDDYGHHPAELKACISSVRELFPGKKIAGVFQPHLYSRTRDFADEFAQSLDLL
                     DAVFLMEIYPARELPVPGVTSSMLLGKINVHEKCLVSDDELLEKLRRAEFDVLITMGA
                     GNIDRFVEPVTQMLKEKIKNKTS"
     gene            complement(5293..6414)
                     /gene="murG"
                     /locus_tag="VFU15_00745"
     CDS             complement(5293..6414)
                     /gene="murG"
                     /locus_tag="VFU15_00745"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010800796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HEU4716324.1"
                     /translation="MSQLKFIISGGGTGGHIFPAIAIANALRQRVPDAEFLFVGAEGR
                     MEMEKVPAAGYRIVGLWISGLQRKITIDNLSFPFKVLSSLRKAKKILREFQPDAAVGT
                     GGYASGPMLRVASKAGVPTLVQEQNSFPGITNKILSKKVDRICVAYEGMERFFPKEKI
                     VFTGNPVRQDILNLEGKKPRGYEMFGLQPGKKVLLVIGGSLGARTINESIKEGLQRLA
                     EKNIQVIWQTGKAFFPAAQEAVKPFEDVGIHAYDFISKMDYAYAAADFVVSRAGASSV
                     SELALVHKPCILVPSPNVAEDHQTKNAMALVNHHAAVLVKDADAKASLVDAVIQLSED
                     ENLRTKLELNIAGLAVKNSADLIASEILALVNGRKGGKK"
     gene            complement(6401..7597)
                     /locus_tag="VFU15_00750"
     CDS             complement(6401..7597)
                     /locus_tag="VFU15_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017731847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative peptidoglycan glycosyltransferase FtsW"
                     /protein_id="HEU4716325.1"
                     /translation="MLNAFKYFKGDKVIWVIVILLSLLSLLAVYSSIVALAYKHHDGQ
                     TEYYLLKHGKIILLGFFLMFLTHRIRYVYFSRISQLMLILSVPLLIYTLFHGATVNEA
                     NRWIAIPGTGLTFQPSDFAKLALIMYVARVVTQKQKELENFREVLIHIMAPVFVICGL
                     ILPANFSTAALLLVTCIILMFIGRVPVKHIAKVLGIAVAAFVLLLMMGKLFPQSKLHR
                     AGVWEARVEEWLKGKDDQDADKSYQANMAKIAIATGGLTGKGAGNSTQRNFLPEAYSD
                     FIFSIIIEEYGSLLGGVGIVLLYLMLLYRAVRVATRSQRTFGTLLAFGVSFSLIFQAM
                     INMAVAVNLFPVTGQSLPFLSMGGTSLLFSSVAIGIILSVSRETEDAGETAPVKKEKG
                     GEVVAA"
BASE COUNT         1629 a         2155 c         1952 g         2038 t
ORIGIN      
        1 gaaacaaccg tcagcgtttc ctctttcgtt tcttcttcct gcttcaccac gaggaaaggt
       61 tcgagcgggt tggttatttc ttctttcttt tcatccttgc cgctgtccga aacgggagca
      121 gccgtatttt ctttcgcttc gatctcagaa agcaggaacg gttccaaagg gttcgcctcc
      181 gccttcacct ctttcatctc ttccgtcacc gaagccaggg gcgtttcagc gactgatttc
      241 gcttcgtcga gattgagcac gatcttctgc tgggcaggct tgcgtgaata ttcgtaggaa
      301 accgggttgt tctggttgaa gccggtggca atgattgtga cactcactgc atcgccaagc
      361 gaagaatcga cgccgtaacc tttgatgatc tcggcggtct gtccggcggc ttcctggatg
      421 taatccgtta tctcgccgag ctcgtccatc gtcatttcct cttcgccgga aacgatgtta
      481 agcagcacgt aacgcgcacc ggtgatgtta ttgtcgttaa gcagcggcga gttgagcgcc
      541 atttccacgg cacgcacagc gcggttttca ccgttcgtta cggcggagcc catgatcgcg
      601 acaccgctgt tacgcatgac ggtgcacaca tcagcgaagt cgacgttgat ctgctgcgtc
      661 aggctgatga tctctgcaat gcttttcgcg gcgatcgtga gcacgtcgtc tgcatgcgcg
      721 aatgcatcgc tgagacgaag gttgccgtac atttcacgca gcttgtcgtt gcagatcacg
      781 aggagcgtat cgcaattctg cttgagctgc tcgagacctt cttcggcctg ctgctttctt
      841 ttctttcctt cgaacgagaa cggtacggtg acgatcgcca ccgtgagaat gccgagctcg
      901 cgtgcgatcc ctgcaatgat cggcgctgca ccggttcctg ttccgccgcc catcccggcg
      961 gtgatgaaca ccatcttggt gttgttggaa aggatgcggc gaatgtcttc gatgttctcg
     1021 atcgccgcat tgcggccaac ttcggggagc gaaccggcgc cacgaccttc cgtgagcgtt
     1081 gcaccgagga cgatcttata gggaaccggg ctcatgtcga gcgcctgctt gtcggtattg
     1141 cagacaagaa aatccacacc tttgatgccc tggcggaaca tgtggttgac cgcgttgctt
     1201 ccgccgccgc caactccgat caccttgatg atcgatgatt gttctggaaa ttcgaatgtc
     1261 atggtgggtt ctgattttat tgcgttagaa tcgtgtgctg agttatcgtt cgtggacccg
     1321 agcggcttcg catttcccgc gatgccgagc ggatcatttg cattttccgt tcctgtaaat
     1381 tcttccggca gttctgcagt gatcccgctt ttgggagaat cgggcagctg cgcaattatt
     1441 ttttccgcgt cgaactcgtg gatgccgggg atcccgtttt ccgggtcttc ggaaacaaaa
     1501 gtttcctcta catcattcac ctcttttttt tcctgcgaaa aatcttctac gggaatttca
     1561 gtgtcgagcg aaaaggaaaa gctgctttcc gtgcctgctt cttcctgcag gttttcctcc
     1621 ccggaaattt tattttcttc cgggttttcg ttcccttcgt tctgatcgtc gaacatttcg
     1681 tagaaatcca tttgctttcg gacgggtaaa aaaaccgttg aaaattactt cagttgcttt
     1741 tttgcaaaac ctttgttacg cgcaacttac taactgtgaa ttgttgatta cgtttctgtt
     1801 aattgcctga atcttcttcg aaccattgct tgcctttgct gaagatctgg tcgaaccagc
     1861 cgccttttgt tttctgcgaa tggttctgaa ccttgttcgt tttttcttcg ttcgctttcg
     1921 atttctgtgt ggcgagcaag ccgcgcatca caagtccaac gcctgttgcg tacatcgggc
     1981 tggtgatctc ttcggagctc gaggcgaggt gctcgttcgg gtaaccgacg cgcgtgtcca
     2041 ttcccgtgat gtattccatc agctgcgtga tgtgcttcag ctgtgcgccg ccgcccgtga
     2101 ccacgatgcc ggcaatgagc ttcttctcga agccggaatt ttttatttca taatacacgt
     2161 ggtcgatgat ctcttccatc cttgcctgga tgatgtgcgc aagattcttc acggaaatct
     2221 ccttgtgcgg ccgcccgcgc agtccgggaa tggaaacaat ttcattttcc tggttctcgc
     2281 ttgcgagcgc tgagccgaat tttattttca gcagctctgc ctgcgttttg atgatgctgc
     2341 agccttcctt gatgtcttcc gtgatgatat ttccgccgaa gggaatgacc gcggtgtgac
     2401 ggataatgcc gtcgtggaaa atggcgatgt ccgttgtgcc gccgccgata tcgaccagca
     2461 cgacgcccgc ttctttttct tcatcgctca ggacggcgtc tgcggaagcg agcggctcga
     2521 gcgtcaggtc tgcggcttcc agtcctgctt tcgccacgca cttgtaaata tttttcgcag
     2581 ccgccacctg gccggtgatg atgtgaaaat tcgcttcgag acgggtgccc gccatcccga
     2641 tcggattttt aattccgact tcattgtcca caatgtattc ctgcgggatc acatgaatga
     2701 tctcttcacc cggcgacatc acgagccggt acatatcttc gataagctcg tcgatatctt
     2761 tctggctgat ctcgtcgtcg atgctcctgc gcgttttcat gccgcggtgc tgcaggcttt
     2821 tgatgtgctg gccggcaata ccgacattga ccactttaat gtcgacgccg gatttgttgc
     2881 tggcttcgtc caccgcctgg cgtatcgcct tcacggtttc atcgatattg agcacgacgc
     2941 cgcgggccac gccgagcgat tccgcctttc ccatcccgag aatttctatt ttcccgtgat
     3001 ctgtgcggcg gcctacaagc gcggcgatct tggttgttcc gatgtcaagt ccgactacaa
     3061 tttcattgtc catggtcgtt attctttttg gtacagacga tttgattttt gtattgaaga
     3121 tctattttcg aataatcatt ccagctgccc gttttcccga gaccttcccg gtagaagaga
     3181 cgaagccggt acaacttatc atcgagatcg cggatgtcgc cgatcacgat gctctggctc
     3241 ccgatgcgcg ggatgagctg aatcccttca tccggcgaga tgaacacctg cgttatctgc
     3301 gcggtgagaa atgaatccgc ttcgagcttg cgtacgatct gccaggcgtc atcgagaaca
     3361 gtaggcgtct ttaatgcgga atcagcggaa attttactga aatcaatatg ctgcatggcc
     3421 gcgtaaccgt caccgaaata accgctcacc aagatcagcg gggctgtata ctcttccgac
     3481 cagggcatga gctttccttt gtcatccatg taataggatt cgccgttgta attcatgaac
     3541 cgcatcacgg gattacgctg cctgatcttc acagttacgt tcccgtctat gctgatgaat
     3601 gcatcgcagc tctccacagc cgggtgcgtc agcagtaatt tttcgatccc gttcacatca
     3661 atgtccgaga tcgactttcc ttccggcatc attgcgtgtg ccgaaagcag ctcacgtata
     3721 tcttccggcc ggataaaaaa atgattcccg ggcggcgaaa tgtccacgta cagctttccg
     3781 catttgcggg cggcatgaga acgagaagca aagctcatgg tgacgatcgc accggcgaat
     3841 gccacaatcc acagacctat gtagatgagc tttttcatga agtcttgttt ttgatctttt
     3901 ccttcagcat ctgcgtgacc ggttccacga aacggtcgat gttcccggcg cccatcgtga
     3961 tcagcacatc gaattccgct cggcgtaatt tttccagcaa ctcatcatcg ctgacgaggc
     4021 atttttcatg cacgtttatt tttccgagca gcatcgacga cgtcacaccc ggaaccggca
     4081 gctctcgcgc cgggtaaatt tccatcagga aaacagcatc gagcaggtcg aggctctgtg
     4141 caaattcatc ggcaaaatca cgtgtgcgtg aatagaggtg cggctggaaa acaccggcga
     4201 tctttttccc cgggaacaat tcgcgcaccg aagagatgca cgctttcagc tcggcgggat
     4261 gatgaccgta atcgtcgata taaacgcatt ccggatcgcg aagatgaata tcgaagcgcc
     4321 gctgcacacc acggaaagag gccagcgcgc ttttcacgga ttcatcacca atgccgaact
     4381 ctttcgccgc tgttacggca gcgactgcgt tttccacgtt gtgccggccc ggcaggccca
     4441 gcttcagtcc ttcgattttt ttgccgcgaa tgacgagatc gaaatggtaa aaaccatctt
     4501 ccacccggat ctcttttgca aacgcatccg acgaatcttc ctcgagcgaa tacgtgagca
     4561 ctttctccgt aacgggaatg ccaagctggt ccagcacttt ccgtcgcgtg atcagtttcc
     4621 gcttcacctg tgaaataaat ttccggtagg actcatccat ctcgttcctg tttccgtaaa
     4681 tgtcgagatg gtccggatcg gctgaagtca cgatcgcgac ttccgggtgc agccagtgga
     4741 atgagcggtc gtattcgtcc gcttccgcaa cgaggatatt ttccccggca ggaacatttt
     4801 ccttcgcgat attttttccg agcagcaggt tcgtgtggta attcccggaa attccaccga
     4861 ggaaggcaga gctgtcgata cccgaagtac ggagcaggtg ggccgtaagc gtggtggtcg
     4921 tggttttccc gtgcgtgcct gcaacggcga tggtcgttga attttccgcg atgatgccga
     4981 gcacccgggc tcgcttcatc agtgtaaatc ccccgtcacg aaagaaattc agctcgcgat
     5041 gatcgggagg caatgccggc gtccagacca ccagcagctt ttctttaccg gcaatatatt
     5101 cagcaggaat atttttcacg tcgtcctcga aatgaatttc catccccgca gctgcgagct
     5161 catccgtcag cgcggtgcgc gtcctgtcgt atcctgccac ttttttccct gctgcttgaa
     5221 aatatcttgc aagcgcgctc atgccgatgc cgccgatgcc gaggaaataa acataccgga
     5281 tggcgttcag cttcattttt ttcctccctt tcttccattg acgagcgcca gtatttccga
     5341 cgcgatcagg tcggcagaat tcttgacggc aagcccggcg atgttcagct cgagcttcgt
     5401 acgcaggttt tcatcttccg aaagctgaat gaccgcatcc accaaacttg ctttcgcatc
     5461 cgcatctttt acaagcacag ccgcatggtg attgaccagc gccatcgcgt tttttgtctg
     5521 gtggtcctcc gccacgttcg gcgacggaac aagaatgcac ggcttgtgca caagcgcgag
     5581 ttcggaaaca gagcttgccc ctgcgcgtga aacgacaaag tcggcagccg catacgcata
     5641 atccattttg gaaatgaaat cgtacgcgtg gatgccaaca tcttcaaacg gtttcaccgc
     5701 ttcctgcgcc gcaggaaaaa atgcttttcc tgtctgccag atgacctgga tatttttttc
     5761 cgccagcctc tgcaatcctt cctttatgct ttcgttgatc gttcttgcgc ccaggcttcc
     5821 gccgatcacc agcaatactt ttttccccgg ctgcaaaccg aacatctcgt atccccttgg
     5881 ttttttccct tcgagattga gaatatcctg ccgaacggga ttcccggtaa aaacaatttt
     5941 ctctttcggg aaaaaccgtt ccattccctc gtacgccaca caaatgcgat cgactttctt
     6001 cgaaagaatt ttattcgtaa tgcccgggaa tgaattctgc tcctgcacga gcgtcgggac
     6061 acccgctttg gaagcaacac gcagcatagg tccgctggcg taaccgccgg tgccgacagc
     6121 cgcatccggt tgaaattcac gaagaatttt tttcgctttc ctgagactgc tcagcacttt
     6181 gaacgggaac gaaagattat cgatcgttat tttccgctgc aggccgctga tccacaggcc
     6241 gacgatcctg tatccggcgg cgggaacttt ttccatctcc attcttcctt cggcgccgac
     6301 aaaaagaaat tccgcatcgg gcacacgttg cctgagcgca ttcgcgatcg caatggcggg
     6361 gaaaatatgg ccgccggttc ctcctccgct gatgatgaac ttaagctgcg acaacctcgc
     6421 ctcctttctc ttttttcacg ggggccgttt ctcccgcgtc ttccgtttca cggcttacgc
     6481 tgagaatgat cccgatcgca acgctggaaa aaagcaacga cgttccgccc atgctgagga
     6541 acggaagtga ttgtcccgtt accgggaaaa gattgacagc caccgccatg ttgatcatcg
     6601 cctggaagat caggctgaac gaaacgccga atgcaagcaa tgttccgaac gtgcgctggc
     6661 tcctcgtcgc cacgcggaca gcgcggtaga gcagcatcag gtaaagcagg acaatcccga
     6721 caccgccaag cagggaaccg tattcttcga tgatgatgga aaaaataaaa tccgaatagg
     6781 cttccgggag aaaattccgc tgcgtactgt tgcccgctcc tttccccgta agtccgcccg
     6841 tggcgatagc gatcttcgcc atgttcgcct ggtaactttt atccgcatcc tggtcatctt
     6901 ttcccttgag ccattcttcc acgcgcgctt cccacacgcc ggccctgtgc agtttggact
     6961 gcgggaataa ttttcccatc atcagcagca gcacgaacgc ggccacggct ataccgagca
     7021 ctttcgcaat gtgcttcacc ggaacacggc cgatgaacat gaggatgatg caggtaacga
     7081 gcagcagcgc cgcagtggaa aaattcgcag gaaggatgag tccgcagata acaaaaacag
     7141 gcgccatgat atgaataagc acttcacgga aattttcaag ctccttctgc ttctgcgtta
     7201 cgacgcgcgc cacgtacatg atcaacgcca gctttgcaaa gtcggaaggc tggaacgtaa
     7261 gacctgttcc gggtatggcg atccagcggt tggcctcgtt caccgtagcg ccgtgaaata
     7321 atgtgtaaat gagcagcgga acagaaagga tcagcatcag ctgcgatatt ctcgaaaaat
     7381 aaacgtagcg gatgcgatgg gtgagaaaca tgagaaagaa cccgagcagg ataatttttc
     7441 cgtgcttgag caggtaatat tccgtctggc cgtcgtggtg cttgtacgca agcgcgacga
     7501 tcgagctgta cacggccagc agcgacaaca gcgagagcag gatgacgatc acccagatca
     7561 ccttgtcacc tttgaaatat ttaaatgcgt tcagcatctc tcagtcgcgt tcgaatttct
     7621 gttttacgat cttccggaat ttgtcgccgc ggttccggta attatcaaat gcgtcgtagc
     7681 tcgggcacga cggtgaaaaa agcactgtct ttattttcgt gctggccaga agcgccgcgt
     7741 ggtcgaccgc ttccttcagc gaacctgcgt gtat
//
LOCUS       DASTSF010000025         3395 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_2001663,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000025 DASTSF010000000
VERSION     DASTSF010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3395
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2001663"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..347
                     /locus_tag="VFU15_00755"
     CDS             <1..347
                     /locus_tag="VFU15_00755"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HEU4716326.1"
                     /translation="MIIGLLFMFMIAMFYRLGPAQRMHKSFFSPGVLAATTLIILTSL
                     AFAWYVNSFGKYNKLYGSIGSIIVLLIWIFYNSMMLLIGFELDAGISAARRKRLSLLQ
                     FEEEEKRKEEEV"
     gene            complement(390..1112)
                     /locus_tag="VFU15_00760"
     CDS             complement(390..1112)
                     /locus_tag="VFU15_00760"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU4716327.1"
                     /translation="MRTVLFLIRKSKVLFMRWRLHVLVEPFSGFLLNLVYLSKMSKWR
                     RKTPSPAFNDFYSGKWDYKKRFRLYEYLLESEKLDGAVRYMEFGVAAGQSFKWWVEHN
                     AHAESTFDGFDTFTGLPEDWNVFKAGAMSTGGILPDVSDQRATFHKGLFQQTLPGYLQ
                     SINDERRKVIHLDADLYTSTLYVLTSVAPWLKKGDILLFDEFAVPRHEFLAYSEFIQS
                     YYIKTELIAAQNNYYFTAFRIM"
     gene            1203..>3395
                     /locus_tag="VFU15_00765"
     CDS             1203..>3395
                     /locus_tag="VFU15_00765"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEU4716328.1"
                     /translation="MIRKLLLVLLLFCGTISIAQVNENAYASKSNALYWKNRPPYEGY
                     WQQDVQYNINATLDDQTDIITGDEDLNYFNNSPDTLAFVYFHLYQNAFQPGSYTDDLH
                     RNNDFLVRYGRYESQKLGTQILSITEDNFTPKTETDNTIMKVWLHKPLLPGGHVSFRI
                     SFKTYFDDGGNIRRRMKMYKTFGYKHYDGVHWYPRMAVYDRKFGWCTDQHLTREFYGD
                     FGTYDVQLTLPNNYILDGTGVMLNENEMLPPDLRRKLDISNFATKPWNSAPSQVIAPD
                     GTTKTWKFHADNVHDFAFTADPLYRIGEVDWNGVRCIALAQEQHCAGWVSAAAYTARV
                     IEVYSKDFGMYAYPKMIVADAQDGMEYPMLTLDGGYDPNYRSLIAHEVGHNWFFGMVG
                     SNETYRALLDEGFTQFLTAYCCVKLGDKYVPHNPYISNYVGRFSDEETTMNQQVYDGY
                     IFDAARGDETVIDNHSDHFNGALRHGGGYGQVYMKTATMLYNLQYVLGDSLFLAAMSH
                     YFNQWKFCHPYVEDFRNSIIQFTHVDLNWFFDEWLTTSQTIDYGIKSVKKADSTDTYN
                     VTFKRYGRMQMPIDFTVFANDGQQYSYYIPNTWYEKQTTATSLPRWIGWDKLKPTYTA
                     RIVVPSGIREIQIDTTHRLADVNMLNNSTKMPVTFVFDSKVYNTPSWEHYSLRGRPDF
                     WYNGYDGLKVGMYLGGDYMNTFHQFNLLFHYNTGIGQSQLKPGTEINRF"
BASE COUNT          858 a          921 c          874 g          742 t
ORIGIN      
        1 ggatgatcat cggcctgctg ttcatgttca tgatcgcgat gttctatcgt ctcggtcctg
       61 cacagcgcat gcacaaaagc tttttcagcc cgggcgttct cgcggccacg acgctcatca
      121 tccttacctc gcttgcgttt gcctggtacg tgaacagctt cgggaaatac aataaattgt
      181 acggatcgat cggctccatt atcgtgctgc tcatctggat cttttacaat tccatgatgc
      241 tgcttatcgg gttcgagctc gacgcaggaa tttccgccgc acggcgcaaa cggctttcac
      301 tgctgcagtt tgaagaggaa gagaaacgga aggaagaaga ggtttgattt cggatttcga
      361 atgtcggatt tcgaatgtcg gatttacaat tacattatcc tgaatgctgt gaaataataa
      421 ttgttctgcg cggcgatcag ttccgtttta atataatacg actggatgaa ttccgaatag
      481 gcaagaaatt cgtggcgggg aacagcaaac tcatcgaaca gaagaatatc tccttttttc
      541 agccagggcg ccaccgacgt cagcacgtac agcgttgacg tgtaaagatc cgcgtcgaga
      601 tgaatgacct tgcggcgctc atcattgatg gactgcaggt aacccggcag cgtctgctgg
      661 aaaagtcctt tgtgaaacgt agcgcgctgg tcgctgacat ccggcagaat tccaccggtg
      721 gacatcgcgc ctgctttgaa aacgttccag tcttccggca ggcccgtgaa cgtatcgaac
      781 ccgtcgaacg tcgattccgc gtgcgcgtta tgctctaccc accatttgaa ggattgtccc
      841 gccgccacgc cgaattccat gtaacgcacg gcgccgtcga gcttttccga ctcgagaagg
      901 tattcgtaga gcctgaaacg ttttttgtaa tcccatttcc ccgaatagaa atcgttgaag
      961 gcaggcgaag gtgttttacg cctccacttc gacattttgg agagataaac gagattcaat
     1021 agaaaaccgc tgaaaggttc caccagcacg tgcaggcgcc agcgcatgaa aagcactttc
     1081 gatttgcgga tgagaaaaag aaccgtgcgc atgaggggcg ctaagttagc aatgataaat
     1141 gaatggcaga cagggattaa cccgtccaga actttatatt tgtaacatac actattcgcc
     1201 agatgatccg gaaacttctc ctcgttctgt tgcttttctg cggaacaatc tccatcgccc
     1261 aggttaatga aaatgcgtac gccagcaaaa gcaatgcgct gtactggaaa aacaggccgc
     1321 cgtatgaagg ctactggcag caggatgtgc agtacaacat caacgcaacg ctcgatgacc
     1381 agacggacat cattacgggc gacgaagacc tgaattactt caacaattca ccggacacgc
     1441 tcgcgttcgt ttatttccat ctttaccaga acgcttttca gccgggatcc tacacagacg
     1501 atctccaccg caacaatgat tttctcgtgc gctacggccg ctacgaatcg cagaagctgg
     1561 gcacgcagat cctttccatc accgaagata atttcacgcc gaaaacggaa acggacaaca
     1621 ccatcatgaa agtgtggctg cacaagccgc tgctgcccgg gggccacgtc agcttcagga
     1681 tctcgttcaa aacctatttc gatgacggtg gcaacatccg ccgccggatg aaaatgtaca
     1741 agaccttcgg gtacaaacat tacgacggcg ttcactggta tccccgtatg gccgtgtacg
     1801 accgcaagtt cgggtggtgc accgaccagc atctcacccg cgaattctac ggcgatttcg
     1861 gaacatacga cgtccagctc acgcttccga acaattacat cctcgacggc accggcgtca
     1921 tgctgaacga gaacgaaatg cttccgcctg acctgcgcag gaagctcgac atttccaatt
     1981 tcgcgacgaa gccgtggaac tccgcaccga gccaggtgat cgcgcccgac ggaactacca
     2041 agacatggaa attccacgcg gacaacgtgc acgatttcgc cttcaccgcc gatccgcttt
     2101 accgcatcgg ggaagtcgac tggaacggcg tgcgctgcat tgcgctggcg caggagcagc
     2161 attgcgcggg atgggtttca gccgcagcct acacggcgcg cgtcatcgaa gtttattcga
     2221 aagatttcgg catgtatgcg tacccgaaaa tgatcgtggc ggatgcgcag gacggtatgg
     2281 aataccccat gctcacgctc gacggaggct acgatccgaa ttaccgttca ctgatcgcgc
     2341 acgaggtcgg gcacaactgg ttcttcggaa tggtgggcag caacgaaacg taccgtgcgc
     2401 tgctcgacga aggattcacg cagttcctca cggcttactg ctgtgtgaag ctcggcgaca
     2461 aatacgttcc gcacaacccg tacatcagca attatgtcgg acgtttcagc gacgaggaaa
     2521 cgacgatgaa ccagcaggtg tacgacggtt acatttttga cgcggcgcgc ggagacgaaa
     2581 cggtgatcga caatcactcc gatcatttca acggtgcgct gcgccacggc ggaggctacg
     2641 ggcaggtgta catgaaaacc gcgacgatgc tgtacaacct gcagtacgtt ctcggcgatt
     2701 cgcttttcct tgcggcgatg tcgcattatt tcaaccagtg gaaattctgc catccgtacg
     2761 tggaagattt ccgaaactcc atcatccagt tcacccacgt cgatctcaac tggtttttcg
     2821 atgaatggct gacgacgtcg cagacgatcg actacggaat aaaatccgtg aagaaagccg
     2881 actcgaccga cacgtacaac gtaacgttca agcggtacgg ccgcatgcag atgccgatcg
     2941 atttcactgt attcgccaat gacggccagc aatacagcta ctacattccc aacacgtggt
     3001 atgaaaagca gacgaccgcc acctcgctgc cgcgatggat aggatgggac aaattaaagc
     3061 caacgtacac cgcgaggatc gtcgttccgt cggggatcag ggagatccag atcgacacga
     3121 cgcaccggct ggcggatgtg aatatgctga acaacagcac caagatgccc gtgacgttcg
     3181 tcttcgattc gaaagtgtac aacacgccgt cctgggaaca ttattccctg cggggaagac
     3241 cggatttctg gtacaatggc tacgacggac tgaaggtcgg gatgtatctc ggcggtgatt
     3301 acatgaacac gttccaccag ttcaacctgc tgttccacta caataccggc atcgggcaat
     3361 cgcagctgaa accgggaacg gaaataaacc gtttc
//
LOCUS       DASTSF010000026         4577 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_420323,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000026 DASTSF010000000
VERSION     DASTSF010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4577
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_420323"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..373)
                     /locus_tag="VFU15_00770"
     CDS             complement(<1..373)
                     /locus_tag="VFU15_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002997818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 factor interaction domain-containing
                     protein"
                     /protein_id="HEU4716329.1"
                     /translation="MTIQEIKNRFGIIGTSPLLDRAIDIAVQVAPTDLSVLITGESGT
                     GKEVFPQIIHHLSARKHGPYIAVNCGAIPEGTIDSELFGHEKGSFTGATEARKGYFEV
                     ADGGTIFLDEVAEMPHATQARL"
     gene            complement(384..548)
                     /locus_tag="VFU15_00775"
     CDS             complement(384..548)
                     /locus_tag="VFU15_00775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716330.1"
                     /translation="MKKKPRISSIDVEKTLRDFPAFVKAREKKMRKKIREEKKYLNEA
                     VREVFKKDSK"
     gene            complement(545..1102)
                     /locus_tag="VFU15_00780"
     CDS             complement(545..1102)
                     /locus_tag="VFU15_00780"
                     /inference="COORDINATES: protein motif:HMM:NF018154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HEU4716331.1"
                     /translation="MTKTIEIFAGPNGSGKTTFGEKGYAKRKDAIYLNPDKIASGLSV
                     TGGEMSQFEAGRVLFKRVEDCLSQNISFAFETTMSGKIWISFIKKAKKRGYKISIYFL
                     FVNSEKLSLKRIRNRVKLGGHDIPKETVHRRFEKTFSNFKTLYAPLADEWTLIDNSKR
                     GKVIAKLEAGTIKVSNKATYKKFFQ"
     gene            complement(1113..1556)
                     /locus_tag="VFU15_00785"
     CDS             complement(1113..1556)
                     /locus_tag="VFU15_00785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716332.1"
                     /translation="MYGDDVDREGGVMKKYAAALLLIFCVAFQARAGTIDFCQVFIKD
                     SLTCSITQNDFGKGVLYLTIPNENDTLDIRYFSGNGGFGGHDFVVRDKKGKMLLTFKN
                     DENDPASCKPFLLPVKNLSLEGSCIYLVSEGEETAVLNIVILPRQ"
     gene            complement(1520..2950)
                     /gene="miaB"
                     /locus_tag="VFU15_00790"
     CDS             complement(1520..2950)
                     /gene="miaB"
                     /locus_tag="VFU15_00790"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020526290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HEU4716333.1"
                     /translation="MSEHEKTIDEQRQGEALVTAPAESGTRKLFLESYGCQMNFSDSE
                     IVASILRNEGFSTTKDFTEADVIFVNTCAIRENAEQRVRQRLNDFKKAKKNKPELVVG
                     VLGCMAERLKEKFLDEEKIVDIVVGPDAYRDLPQLIGKAEGGHKAVNVILSKEETYAD
                     ITPVRLDSNGVTAFISIMRGCDNMCAFCVVPFTRGRERSRNPESIVQEAKDLFEKGYR
                     EVTLLGQNVDSYIWAGGGLKKEILTEEQKAAAVNFAQLLEMVAQVSPDLRIRFSTSHP
                     KDMTDDVLHMMAKYENICNYIHLPVQSGSSRVLELMNRGYTREWYMERVSAIRRIIPD
                     CGISADIITGFCTETEEDHAETLSLMEWAEYDFSYMFAYSERPKTLAERKFKDDVPEE
                     IKKRRLQEVIALQQKLSEAKTKQHVGKVHRVLIEHTSKKSGDMWMGRNSQNIVVVFPK
                     ENFRPGDYANVLVETCTATTLIGRAV"
     gene            3208..>4577
                     /gene="topA"
                     /locus_tag="VFU15_00795"
     CDS             3208..>4577
                     /gene="topA"
                     /locus_tag="VFU15_00795"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003001121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEU4716334.1"
                     /translation="MAKNLVIVESPAKAKTIEGFLGKDFLVKSSFGHVRDLAKKGLAV
                     DTEKGFTPQYEISPDKEKVVSELRKLAKDAEIVWLATDEDREGEAISWHLSEALKLDE
                     KRTKRIVFHEITKPAILKAIQAPRSIDNHLVDAQQARRILDRLVGFELSPILWKKVKP
                     SLSAGRVQSVAVRLIVEREREIQNFRSVSSYKVSGIFSANGTSFKADLKNGFATVEEA
                     KAFLQKCSSATFTVGSLETKPGKKSPAAPFTTSTLQQEASRKLGFSVRRTMSVAQRLY
                     ESGKITYMRTDSVNLSEIAVEQAKQVIGKNYGDKYIHPRQYKTKSKGAQEAHEAIRPT
                     YLENSSVDAERDEARLYDLIWKRTIASQMSDAELEKTTVVIEVSTAAEHFVAQGEVIK
                     FDGFLKVYMESSDEDGEEKENAEGTLPSMQNGQKLNAGTITATQRFTHHPARYTEASL
                     VKKLEEL"
BASE COUNT         1070 a         1182 c         1129 g         1196 t
ORIGIN      
        1 gcaggcgcgc ctgcgtggca tgcggcattt ccgccacttc gtcgaggaag atcgttccgc
       61 cgtcagccac ttcgaaataa cccttgcgtg cttccgtcgc accggtgaac gaaccttttt
      121 catgaccgaa cagctccgaa tcgatcgttc cttcgggaat ggcgccgcag ttcaccgcga
      181 tgtacggacc gtgcttgcgc gcggaaagat gatggatgat ctgcggaaaa acttcttttc
      241 ccgttccgct ttcaccggtg atgagcacgg aaaggtccgt gggcgccacc tgcaccgcga
      301 tgtcaatcgc gcggtcgagc aggggcgaag tgccgatgat gccgaaacgg ttttttattt
      361 cctggatagt catttatgtg ctcctatttc gaatcctttt taaaaacttc acgaacggct
      421 tcgttcaggt attttttttc ctccctgatt tttttacgca tttttttttc ccgcgctttt
      481 acaaatgcag ggaagtcgcg aagcgttttt tctacgtcaa tggaggatat cctgggtttc
      541 tttttcattg gaagaatttt ttgtaagtcg ctttgttgct gacttttatt gtgccggcct
      601 ccagctttgc aatcactttc cctcttttac tgttatcaat aagcgtccat tcatcagcaa
      661 gcggggcgta taatgtctta aaattcgaaa atgttttttc aaacctcctg tgaaccgttt
      721 ctttcgggat gtcatgacca cccagtttaa cccggttcct gatcctcttc aatgaaagtt
      781 tttctgaatt cacgaacagg aaatagatcg agattttgta tccccgtttt tttgcttttt
      841 taatgaacga gatccagatt ttgcccgaca tggttgtttc aaacgcgaat gaaatattct
      901 gcgacaggca atcttcgact cttttgaata aaacccttcc cgcttcgaat tgactcatct
      961 caccgccggt tacagaaagc ccgctcgcaa tcttgtcggg attaaggtaa atcgcatctt
     1021 ttcttttcgc ataacctttc tcaccgaaag tcgttttacc actcccgttc ggccctgcaa
     1081 atatttcgat ggttttggtc attgtgaaaa aattattgac gcggtaaaat gacaatatta
     1141 agtactgctg tttcttcccc ctcgcttaca agataaatgc aacttccttc cagggacagg
     1201 ttctttacgg gaagaagaaa tggtttgcag gaagcgggat cgttttcatc gtttttaaaa
     1261 gtcaacagca ttttaccttt tttatcacgc acaacaaaat catgcccgcc gaatccacca
     1321 ttaccgctga aatatcttat gtcaagcgta tcgttttcat tcgggatggt gagataaagt
     1381 acaccttttc cgaaatcatt ttgggtaatg gaacaggtga gcgagtcttt aatgaatacc
     1441 tggcaaaagt caatggtgcc ggcccgggct tgaaaagcaa cacagaaaat cagcaacaaa
     1501 gctgcagcgt actttttcat cacaccgccc tccctatcaa cgtcgtcgcc gtacatgttt
     1561 ccaccaggac gttcgcgtaa tcaccgggcc tgaaattctc cttcggaaaa accaccacga
     1621 tgttctgcga gttccgtccc atccacatgt ctcctgattt tttcgatgtg tgctcgatca
     1681 gcacgcggtg cacttttccg acgtgctgtt ttgtttttgc ttcggataat ttctgctgca
     1741 atgcgatcac ttcctgcagg cggcgttttt tgatttcttc cggaacatca tctttgaatt
     1801 tccgctccgc cagtgttttc gggcgctcgc tgtacgcgaa catgtagctg aaatcgtatt
     1861 ccgcccattc catcagcgaa agcgtttccg cgtggtcttc ttccgtttcg gtgcagaacc
     1921 ccgtgatgat gtcggcggaa atgccgcagt ccggaatgat ccttcgtatc gcgctgacgc
     1981 gttccatgta ccactcgcgc gtgtagccgc ggttcatcag ctccagcacg cgcgagcttc
     2041 cgctctgaac gggaaggtgg atgtagttgc agatgttctc gtatttcgcc atcatgtgca
     2101 gcacgtcgtc cgtcatgtct ttcgggtgcg aggtgctgaa acggatgcgg agatccggcg
     2161 aaacctgcgc aaccatttcc agcagctgtg caaaattcac ggcagccgct ttctgctctt
     2221 cggtgagaat ttcttttttc agtccgccgc cggcccagat gtaggaatcc acgttctgcc
     2281 cgagcagcgt cacttcacgg tagccttttt cgaaaagatc tttcgcttcc tgcacaatgc
     2341 tttccgggtt gcgcgaacgt tcccgcccgc gtgtgaacgg aacaacgcag aatgcgcaca
     2401 tgttgtcgca tccccgcatg atgctgatga aggccgtcac tccattgctg tcgagacgca
     2461 cgggcgtgat atcggcgtag gtttcctctt tcgaaagaat gacgttcacc gctttgtgcc
     2521 cgccttcagc tttgccgatc agctgcggga gatcacggta ggcatcggga ccgacgacga
     2581 tatcgacgat cttttcttcg tcgaggaatt tttctttcag ccgttccgcc atgcagccga
     2641 gcacgcccac gacgagctcc ggcttgttct tcttcgcttt tttgaaatcg ttcagccgct
     2701 ggcggacgcg ctgctccgca ttttcacgga tggcgcacgt gttgacgaaa atgacgtccg
     2761 cttcggtaaa gtctttcgtt gtggaaaatc cctcattcct gaggatcgac gctacgatct
     2821 cgctgtcgga aaaattcatc tggcagccgt agctttccag gaaaagcttc cgggtcccgc
     2881 tttccgcggg cgcggtcacc agggcttcgc cctgtctctg ctcatcaatt gttttttcgt
     2941 gctcgctcat tttttcaggg aaacaaaatt accaaaaaac aagctgcgct gacaaaatgg
     3001 catagcttct taacagcctt ttaatttttt tttctggcgc cctatatata tataaatata
     3061 tatatagacc cgcccgggcg gaaaaaatga tttggaagtg tccgttctgt ttttttttct
     3121 ttgcggctct tgaaacggga atccggcgtg gcaggctgtt cccttttccg gcaaataaag
     3181 gtgaagttgt actgaacaaa tttgattatg gctaagaatc ttgtgatcgt tgagtcgcct
     3241 gcaaaggcaa agacaataga aggtttcctg ggtaaggact tcctggtaaa atccagtttc
     3301 ggtcatgtgc gcgacctggc gaaaaaaggt ctcgccgtgg atacggaaaa aggatttacg
     3361 cctcaatatg aaatttcacc ggacaaggaa aaagtggttt cggagctgag gaagctggcc
     3421 aaggatgcgg agatcgtctg gctcgcgact gatgaggacc gcgaaggaga agcgatttca
     3481 tggcacctgt cggaagcgct caagctcgat gagaaaagaa cgaagcgcat cgtgtttcac
     3541 gagatcacga agccggccat cctgaaagcc atccaggcac cgcgcagcat cgataatcac
     3601 ctcgtcgacg cacagcaggc acggcgcatt ctcgaccgtc ttgtgggttt cgagctttct
     3661 cccatcctct ggaagaaagt gaaaccgtcg ctttccgccg gacgcgtcca gagtgtggcc
     3721 gtacgtctca tcgtcgaacg cgaacgcgag atacagaatt tcagatcggt ttcttcctat
     3781 aaagtctccg ggattttctc tgcgaacgga acatcgttca aagcggatct gaaaaacgga
     3841 tttgcaacgg tcgaggaagc gaaagcgttc ctgcagaaat gttcttcggc aacgttcacc
     3901 gtaggatcgc tcgaaacgaa acccgggaaa aaatctcccg cagcgccctt caccacgtca
     3961 acgctccagc aggaagccag ccgcaagctc ggattttccg tgcgccgcac gatgagtgtt
     4021 gcgcagcgcc tgtacgaaag cgggaaaatc acatacatgc gaaccgattc cgtcaatctt
     4081 tccgagatcg ccgtggagca ggcgaaacag gtgatcggga aaaattacgg cgataaatat
     4141 attcacccgc gacagtacaa aacgaaaagc aaaggcgcgc aggaagcgca cgaggcgatc
     4201 cgcccgacgt acctcgagaa cagctcggtg gacgcggaac gggatgaagc aagattgtac
     4261 gatcttatct ggaaacgcac gatcgcgtcg cagatgagcg atgcggagct ggaaaaaaca
     4321 acggttgtca tcgaagtttc tacggcggcg gagcactttg ttgcgcaggg agaagtgata
     4381 aaattcgacg ggttcctcaa agtgtacatg gaatcgtccg acgaagacgg ggaggaaaaa
     4441 gaaaacgcgg agggaacgct cccgtctatg cagaacgggc agaagctgaa tgcgggtacg
     4501 attaccgcca cgcagcgctt cacgcaccat cccgcgcgtt atacggaagc aagtctcgtg
     4561 aaaaagctcg aagagct
//
LOCUS       DASTSF010000027         2523 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1315722,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000027 DASTSF010000000
VERSION     DASTSF010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2523
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1315722"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1558
                     /gene="ftcD"
                     /locus_tag="VFU15_00800"
     CDS             <1..1558
                     /gene="ftcD"
                     /locus_tag="VFU15_00800"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010259870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HEU4716335.1"
                     /translation="GKATNRTVVTFVGEPEHVTEAAFRAIKTAGELIDMSKHKGEHPR
                     MGATDVCPLIPVSGISMEETTAYAKKLAERVGSELHIPVYLYEYAQADKKRSNLSVIR
                     AGEYEGFFKKIKQPGWKPDYGPAEFDAKRGATVIGARDFLVAYNINLNTTSTRRANAI
                     AFDVREAGRTKKDAKGNTVKDADGNPVVIPGTLKSVKAIGWFIEEYGVAQISMNLTNI
                     NITPVHVAFEEVNKKAMERGIRVTGSELVGLIPLKSLLDAGRYFLEKQERSIGVPEKE
                     LIRIAVKSLGLDEMGPFKPEERIIEYMLRDAAQSKLVSMSLAAFSDETSSESPAPGGG
                     SISAYMGSLGAALGTMVANLSSHKRGWDDRWKEFSDWAEKGQYYKDLLLRLVDEDTNA
                     FNRIMTAFGLPKSNEEEKTKRTKAIQEATKYATEVPFRVMQAAYDSMEMIRAMAETGN
                     PNSVSDAGVGALAARSAVMGAYLNVKINAAGLEDKKFAADILAKGKDIQEKAMKAEAE
                     ILAVVNGKIG"
     gene            1623..>2523
                     /locus_tag="VFU15_00805"
     CDS             1623..>2523
                     /locus_tag="VFU15_00805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716336.1"
                     /translation="MARVHVWIDRFPVLRKIIYFFPVQLILVQLKKNPVLIAFWIILF
                     GFLSGSIASRYGVFYLFVDPEYLGKVNFLSYLILGFACGGFVMAYQISCYIHNAFRFP
                     FLATLRKPFLRFSTNNFIIPFSFQLTYLILALKFLSKEDFSSWQMTGDVLGFIIGNAI
                     FILGSLLYFFRTGKDMESLYGVVISEREMPKPKRVILNRDPNEKTLKWKTITPGKEAR
                     DWTVHSYIGNWWKIKRARPFEHYDKELLNRIFIQNHGKAVLFEIIVLGTLLIMGIFRQ
                     VPVMMIPAGASIFLLFTLYLMFSA"
BASE COUNT          615 a          692 c          659 g          557 t
ORIGIN      
        1 gggcaaagcg accaaccgca cggtcgtcac gtttgtcggg gaaccggaac acgtgaccga
       61 agctgcgttc cgcgccatca aaacggccgg cgagctcatc gacatgtcga agcacaaagg
      121 cgagcacccg cgcatgggcg ccaccgacgt gtgcccgctc atccccgttt ccgggatttc
      181 catggaagaa acaacggcgt acgcgaaaaa gcttgccgaa cgcgtgggca gcgaattgca
      241 tattcccgtt tacctctacg aatacgcgca ggcagataaa aaacgcagca acctttccgt
      301 tatccgcgca ggcgaatacg aaggcttttt caaaaaaata aaacagcccg gatggaaacc
      361 ggattacggt ccggcggaat tcgatgcgaa acgcggcgca accgtgatcg gcgcacgtga
      421 ttttcttgtt gcctacaaca tcaaccttaa cacgacttcc acgcgccgcg cgaatgcgat
      481 cgcgttcgac gtgcgcgaag cgggacgtac gaaaaaagac gcgaaaggca ataccgtaaa
      541 agatgcggac ggaaaccctg tcgtcattcc cggcacgctg aaatcggtga aagccatcgg
      601 ctggttcatt gaagaatacg gcgtcgcgca gatctccatg aacctcacga acatcaatat
      661 caccccggtg catgttgcat tcgaagaggt gaataaaaaa gcgatggaac ggggcatccg
      721 cgtaaccggt tccgagcttg tcggcctcat tccgctgaaa tcactgctcg acgcgggtcg
      781 ctattttctc gaaaagcagg aacgttccat tggcgttccg gaaaaggagc tgatcaggat
      841 cgccgtgaaa tcgctcgggc tcgatgagat gggtccgttc aaacctgaag agcgcatcat
      901 cgaatacatg ctgcgcgatg ctgctcagtc gaagctcgtc tccatgtcgc tcgctgcatt
      961 ttccgatgaa acgtccagcg aaagtccggc tcccggcggc ggttccattt ccgcctacat
     1021 gggttcgctg ggcgctgcgc tgggaacaat ggtggccaac ctttcttcgc acaagcgcgg
     1081 gtgggacgat cgctggaaag aattttcaga ctgggcggaa aagggccagt attacaaaga
     1141 ccttctcctc cgtcttgtcg acgaggacac gaatgcgttc aaccgcatca tgaccgcttt
     1201 cggtcttccg aaatcgaacg aagaagaaaa aaccaagcgc acgaaagcca tacaggaagc
     1261 cacgaaatac gcaacggaag ttccgttccg cgtgatgcag gcagcgtatg attcgatgga
     1321 aatgatccgg gcgatggcgg agaccggcaa ccccaattcc gtttccgacg ccggcgtggg
     1381 agcgctcgcc gcacgatcgg cggtgatggg cgcctacctg aatgtaaaga tcaacgcggc
     1441 aggactcgaa gacaaaaaat tcgccgcgga cattctcgca aaaggaaaag acatccagga
     1501 aaaagcaatg aaagcggagg cagaaatact ggctgtcgtg aatgggaaga tcgggtgacc
     1561 gttatcatcg cggttaaact ttccacgccc gtatttcgtt taacagaaca gaactgcgtg
     1621 ttatggcccg tgtgcatgtg tggattgacc gttttcccgt cctgagaaag atcatttact
     1681 tctttcccgt acagctgatc ctcgtacagc tgaaaaaaaa ccctgtgctg atcgccttct
     1741 ggatcatcct tttcgggttt ctttcaggct ccatcgcgtc gcgttacggt gtattttatc
     1801 ttttcgtcga cccggagtac ctcgggaaag tgaatttcct ttcctacctc atacttggtt
     1861 tcgcctgcgg cggattcgtt atggcttacc agatctcatg ctacatccac aacgcattcc
     1921 gttttccgtt cctcgccacg ttgcggaaac cttttctccg tttttccacg aacaacttca
     1981 tcattccttt ttccttccag cttacgtatc tcattcttgc cctgaaattc ctttcgaagg
     2041 aagatttttc ctcgtggcag atgaccggcg atgtgctcgg attcatcatc ggcaatgcca
     2101 ttttcatcct gggctcgctg ctttattttt tccgcaccgg gaaagacatg gaatcgcttt
     2161 acggcgtagt gatcagcgaa cgcgaaatgc cgaagccgaa gcgcgttatt ctcaaccgcg
     2221 atccgaatga aaaaacgctg aagtggaaaa ccatcacgcc gggcaaggaa gcgcgcgact
     2281 ggaccgttca cagctacatc gggaactggt ggaagataaa acgtgcacgc ccgttcgagc
     2341 attacgacaa agagctgctg aaccgcattt tcatccagaa ccatgggaaa gcggtgctgt
     2401 tcgagatcat cgtgctcggg acgctcctca tcatgggaat tttcaggcag gtcccggtca
     2461 tgatgatacc cgccggggcg agcattttcc ttttgttcac gctttacctg atgttctccg
     2521 ccg
//
LOCUS       DASTSF010000028         2755 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_868082,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000028 DASTSF010000000
VERSION     DASTSF010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2755
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_868082"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(73..1113)
                     /locus_tag="VFU15_00810"
     CDS             complement(73..1113)
                     /locus_tag="VFU15_00810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716337.1"
                     /translation="MKQRILSLFPATLIIIFTGCFIFFSFFYPLLADDFRFREVLQTH
                     SIRSAAFLFYTDSNGRLASHFFLCTVFALFSGSRLLMFVYRLLMLYAFVFSLAFLLKN
                     YLHRFRNTQLKFAGSVYWSAFITSVFFFIFFAGRMETWFWISATGVYLASLIISMNAF
                     ALLLKNTNAAAFFSALLFFLAGGFSESFALTYFVILLLLCIGTIKTKRIRLLHVALCM
                     LSIAGGIALNVFSHGLSNRLGWLPEFHLLQALKNTLHSLGFLLLRWQYLPVAVLTAGL
                     FTWYANTFYPGKFVLKDLLRQGGIVLAVIAFSFAVPCYVLSDIVPDRAASLGYLCGVL
                     FLFDFFLFRKLN"
     gene            complement(1129..1443)
                     /locus_tag="VFU15_00815"
     CDS             complement(1129..1443)
                     /locus_tag="VFU15_00815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716338.1"
                     /translation="MDTGNTQNTGNTEREDRIKRIWTIVSGLYSAGVIGIIAYWMFTE
                     SGPPQDICEMQANWFSDHKCYIALNFGLPLIVMMIPLLVARFFIERSTGVKLPMGRNR
                     YR"
     gene            complement(1525..2199)
                     /locus_tag="VFU15_00820"
     CDS             complement(1525..2199)
                     /locus_tag="VFU15_00820"
                     /inference="COORDINATES: protein motif:HMM:NF042673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6629 family protein"
                     /protein_id="HEU4716339.1"
                     /translation="MCFSASASFGAGILLTGIGAGSIKQVRQPAELFFASIPLIFGVQ
                     QLSEGFVWLSLSRPGYVPLQHVATFLFLFFAQVVWPVWVPLSIMLLKKKERRKLSQKL
                     LTATGALVSAYLAYCLFRFHVEAKIIGYHISYEQDYPEALATYGGLLYIIATIAPPFF
                     SDIRRMWMLGAAILISYIITELFYTDYIVSVWCFFASVISIAVWVIMYKLKKTNAASE
                     THMPLS"
     gene            complement(2463..>2755)
                     /locus_tag="VFU15_00825"
     CDS             complement(2463..>2755)
                     /locus_tag="VFU15_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136869.1"
                     /note="heat shock protein 70; assists in folding of
                     nascent polypeptide chains; refolding of misfolded
                     proteins; utilizes ATPase activity to help fold;
                     co-chaperones are DnaJ and GrpE; multiple copies in some
                     bacteria; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEU4716340.1"
                     /translation="TEKQLKEFGDKLPADKKQPIEDGLAELKKAHEARDIAGIETSMA
                     KLQTVLQGMYEAMQNQAGGSSENPNANANPQNDGKDSTVTDVDYEEVKDDKK"
BASE COUNT          739 a          680 c          741 g          595 t
ORIGIN      
        1 aaagcctttc gcgttctctt tgttaaaaga aaacttcata ctcccgttcg gaagaatttt
       61 tgatttgaat gatcagttca gtttcctgaa caggaaaaaa tcaaacagga aaagcacgcc
      121 gcacaaatat cccagcgaag ccgcccggtc cggaacaata tccgacaaca catagcacgg
      181 cacggcgaat gaaaatgcga tcacggcaag cacgatcccg ccttgtcgca gcaggtcttt
      241 caggacgaat tttcccgggt aaaacgtatt cgcgtaccag gtaaaaagtc ccgcagtaag
      301 aacagcgacc ggcagatact gccagcggag gagaaggaaa cccagcgaat gcagcgtgtt
      361 tttcagcgcc tgcagcaggt gaaattcagg cagccacccg agcctgttcg acagtccgtg
      421 tgaaaaaaca ttcagcgcta ttcctcccgc aatgctcagc atgcaaagcg cgacgtgaag
      481 cagcctgatc cgttttgttt ttatcgttcc aatacaaaga agaagcagga tcacgaaata
      541 ggtcagcgca aacgattcgg agaacccgcc ggcaagaaaa aaaagcagcg cggaaaagaa
      601 cgcagctgca ttcgtgtttt tcagcagcag cgcaaatgca ttcatggaaa tgatcagcga
      661 agcgaggtaa acgcccgtgg ccgagatcca gaaccaggtt tccattcttc ccgcgaaaaa
      721 aataaaaaag aaaaccgagg tgatgaatgc ggaccagtaa acggaaccgg cgaatttcaa
      781 ttgcgtattc cggaaacgat gcaggtagtt tttcagcagg aaagccagcg aaaagacgaa
      841 tgcatacagc atcagcaggc ggtagacgaa catcagcagt cggcttccgc tgaataaagc
      901 gaagacggtg cacaggaaaa aatgcgaagc gaggcgcccg ttggaatcag tgtaaaataa
      961 aaaagccgca gaacggatgg aatgggtctg cagcacttcc cggaagcgga aatcgtcggc
     1021 cagtaacggg tagaagaagg agaaaaaaat gaagcagccc gtgaaaataa tgatgagcgt
     1081 cgcgggaaaa agggaaagga ttcgttgctt cattaatttc cgggacgctt accggtaacg
     1141 gttccttccc atcggcagct tcacgccggt tgatctttct atgaaaaatc ttgcgacgag
     1201 cagggggatc atcatgacga tcagcgggag gccgaagttc agcgcgatgt agcatttgtg
     1261 gtcggaaaac cagttggcct gcatctcgca gatgtcctgc ggcggaccag attcggtaaa
     1321 catccagtaa gcgattatgc cgataacgcc tgcggaataa agtccgctca cgatcgtcca
     1381 gatgcgcttg atgcggtctt ccctttccgt gtttccggtg ttctgcgtat tgcctgtgtc
     1441 catagggttt ttattttcaa agctaaggaa tcaggcgaaa gttgcaaggg tgaaacacgc
     1501 gcgggatcgc tgcctcgcag cgtttcagga aagcggcatg tgcgtttctg aggccgcgtt
     1561 cgtttttttc agtttgtaca tgatcaccca taccgcgatg ctgatgaccg aagcgaaaaa
     1621 gcaccacacg gaaacaatgt aatccgtgta aaacagttcc gtgatgatgt aggaaatgag
     1681 aatggcggcg cccagcatcc acatgcgcct gatgtctgaa aaaaacgggg gagcgatggt
     1741 ggcgatgatg taaagcaggc ccccgtaagt ggcaagcgct tccgggtaat cctgctcgta
     1801 ggaaatgtga taaccgatga tcttcgcttc gacgtgaaac ctgaaaaggc agtatgccag
     1861 gtacgcggat acgagcgccc cggtcgcggt cagcagtttc tgcgacagtt ttcgccgctc
     1921 ttttttcttc agcagcatga tcgacaaagg gacccatacg ggccagacga cctgcgcaaa
     1981 gaagaggaac aggaacgtgg ccacgtgctg cagcggaaca tagccgggcc gtgaaagaga
     2041 aagccaaacg aaaccttcgg aaagctgctg cacgccgaag atcagtggaa tggacgcgaa
     2101 gaaaagctcc gccggttgcc gtacctgttt tatagacccg gcgcctatgc cggtaagaag
     2161 tattcctgcc ccgaagcttg cactggctga aaaacacatg ggtttcgtgt ataaagtgaa
     2221 aagacctcgt cgccgggaac agcgatcgcc tgcggccgga caaagcggaa ttgtttttcg
     2281 cgggatccgg tcgcctcgaa gattcagcgt gtaatgctca ggaaggaaat cgaaacctta
     2341 ccgaaggtac gctattaaac gcaggagcga aagggaagaa acagcgcagg tctttacttt
     2401 ctccaataaa aaatcccgga ctcttccgga gcccgggatt tgttttttgg atgttggaat
     2461 ttttacttct tatcgtcctt cacttcctca taatccacat ccgtcaccgt cgaatctttt
     2521 ccgtcgtttt gcggattggc attcgcgttc ggattttcgc tgctgccgcc ggcctggttc
     2581 tgcatggctt catacattcc ctggagcacc gtctgcagct tcgccatgga cgtttcgatg
     2641 ccggcgatgt cgcgcgcttc gtgcgctttt ttcagttcgg cgagcccgtc ctcgatgggc
     2701 tgtttcttat cggccggaag tttgtcgccg aattccttca gctgtttttc cgtct
//
LOCUS       DASTSF010000029         4755 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1315685,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000029 DASTSF010000000
VERSION     DASTSF010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4755
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1315685"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..314
                     /locus_tag="VFU15_00830"
     CDS             <1..314
                     /locus_tag="VFU15_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020527384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716341.1"
                     /translation="KEGLGPNFTDDYWLHGGSIQDVFKSIKYGWTDKGMKSWEQDLKP
                     VEIQEVASYVKSLRGTNPPNAKEKQGELYIEEGEKPLQNTDSVKTDSAAAKPPADSLQ
                     K"
     gene            323..1801
                     /gene="ccoG"
                     /locus_tag="VFU15_00835"
     CDS             323..1801
                     /gene="ccoG"
                     /locus_tag="VFU15_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014679707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HEU4716342.1"
                     /translation="MTEEGITVDKKNQEAAQDESFRDSIATINKAGRRNYIFPKKPHG
                     RLYNLRTITSLVYLAVFFTLPFIKIGGDPLFMFNVVERKFILFGQIFWPQDFVLFGLG
                     MITFIVFIVLFTVVFGRVFCGWACPQTIFMEMVFRKIEYLIDGDATEQRRLDQLPWNS
                     YKIRKRALKWGIFYVISFIIANFFLAYIIGMDGLLKIIREPLSMHVGGFISLIIFSFV
                     FFGVFAWFREQACLIVCPYGRLQGVMLDRNSIVVAYDHVRGEPRGHLHKNEKRDLGDC
                     VDCSLCVKVCPTGIDIRNGTQLECVNCTACIDACDEVMEKVHKPKGLIRYASESSIAD
                     KKQLRFTPRIKAYTFLLLLLVGVEVFLLAGRSDVDATILRAGGQLFQEQPGDKISNLY
                     TVRVINKTRNDYPITFRLESGDGEIQMVGKPLMVQKEGKGETEFFILRDKTDIHSRKT
                     KIRIGVYSGDKKLESVSTNFLGPVYVPGEDHHEEEEKNDHHD"
     gene            1813..2250
                     /locus_tag="VFU15_00840"
     CDS             1813..2250
                     /locus_tag="VFU15_00840"
                     /inference="COORDINATES: protein motif:HMM:NF017559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HEU4716343.1"
                     /translation="MNWGLRIVILYSAFVALILFLVIQTMKQKVDLVSDDYYQKELVY
                     QDQIDKMNNAKELSVQPVVSADAHVVRITFPHADSAANVTGVITFYRPSDSSKDLTEK
                     ISAGTDGVQVLSSDSLEKGLYQLQMNWSVAGKNYYNEIPVYIP"
     gene            2252..2962
                     /locus_tag="VFU15_00845"
     CDS             2252..2962
                     /locus_tag="VFU15_00845"
                     /inference="COORDINATES: protein motif:HMM:NF024778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEU4716344.1"
                     /translation="MFPVAALILGLLGSLHCAGMCGPLVLAMPLNRKNSATAAAGLTV
                     YNLGKALSYALIGAVSGAAGSAVKWAVGQQVLSVAAGTFILLVLLAGLFGTRVKLPRQ
                     VEKVFSFVRNSIGKLFRQKGTRANFLIGAMNGFLPCGLVYAAVAGAAASGSWWNGAVF
                     MFVFGIGTMPALFALGLAGSKLTFSFREKLRRVVPVFVAVMAMLLILRGLGLGIPLVS
                     PSLHEGKVVCPHCQHRQK"
     gene            3037..4386
                     /gene="hemN"
                     /locus_tag="VFU15_00850"
     CDS             3037..4386
                     /gene="hemN"
                     /locus_tag="VFU15_00850"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019945601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEU4716345.1"
                     /translation="MNSSLIRKYDVAAPRYTSYPTVPCWNAGKPSEEQWRMAVKEAAQ
                     RDGSISLYIHLPFCESLCTYCACNTRITKNHAVEEPYIENILREWKMYADILPEEVEI
                     SELHLGGGTPTFFSPQHLDRLMSGLKKEIRFSAGASLSFEAHPANTTTAHLETLFRHG
                     FRRMSLGVQDFNPTVQKLINRMQSEAQVSSVTNLARIIGYTSVNFDLIYGLPKQTEES
                     IAETIKTVIRIRPDRIAFYSYAHVPWMKPGQRAYSEADLPEGEKKRALYEKGRALLEK
                     SGYSEIGMDHFALPGDELLHAAVSGTLHRNFMGYTEKATSLLIGLGVSAISDAGTMFV
                     QNEKTVEAWSLHIGKNEMPFFKGHVLDYEDLVLRKHISEIMCAGETAFSANGEPAAFY
                     EGLERLKELVADKLVRVKNNKITVTKSGKAFLRNICMCFDARLHRNRPGDKVYSKSA"
     gene            4447..>4755
                     /locus_tag="VFU15_00855"
     CDS             4447..>4755
                     /locus_tag="VFU15_00855"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEU4716346.1"
                     /translation="MSQLEMRDAMFRYATEGILICDNDGVIQLTNPAVRNMFGYEEND
                     LKGQKIEVLLPQRHRRAHIGMRTDYSRRPEPRKMGYGRDLFATKKDGTEIMVEVSLSP
                     F"
BASE COUNT         1176 a         1193 c         1308 g         1078 t
ORIGIN      
        1 gcaaagaagg tctcggtccg aatttcacgg atgattactg gctgcacggc ggcagtatcc
       61 aggatgtttt caaatccatc aaatacgggt ggacggacaa aggaatgaaa tcgtgggagc
      121 aggatcttaa gcccgtggaa atacaggaag tggcgagcta tgtgaaatca ctgcggggaa
      181 ccaatcctcc caatgcgaaa gaaaaacagg gagagctgta tatcgaggaa ggcgaaaaac
      241 cattgcagaa taccgatagt gtgaaaacag acagcgctgc agcgaaaccg cccgccgact
      301 cgcttcaaaa ataatttcag tcatgacgga agaagggatc accgttgata aaaaaaacca
      361 ggaggctgcg caggatgaat cgttcaggga ttccatcgcg acgataaaca aagccggccg
      421 gagaaactat attttcccga agaaaccgca cgggcggtta tacaatctcc gcacgatcac
      481 aagcctcgtt tacctggccg ttttctttac gcttccgttt atcaagatag gaggcgaccc
      541 gcttttcatg ttcaacgtgg tggagcggaa attcatcctg ttcgggcaga tcttctggcc
      601 gcaggatttc gtgctgttcg gcctcggcat gatcacgttc attgtgttca tcgtgctgtt
      661 tacggtggtg ttcggacgtg tgttctgcgg ctgggcctgt ccgcaaacga tcttcatgga
      721 aatggttttc cgcaagatcg aatacctcat cgacggagat gcaacagagc agcgcaggct
      781 cgaccagctt ccctggaatt cctataagat ccgtaagcgg gcgctgaagt ggggtatatt
      841 ctacgtgatc tcattcatca ttgcgaattt ctttcttgca tacatcatcg ggatggacgg
      901 gttgctgaaa ataatccgcg agccgctatc catgcatgtg ggcggattta tttcactcat
      961 catcttttct ttcgttttct tcggcgtatt tgcctggttc cgcgagcagg cctgtctcat
     1021 tgtttgtccg tacggacggt tgcagggcgt gatgctcgac cgcaattcca ttgtcgtcgc
     1081 gtatgatcac gtgcgcgggg aaccacgcgg gcacctgcac aaaaacgaaa aacgcgacct
     1141 cggcgattgt gtggattgct cgctgtgcgt gaaggtctgc ccgacgggaa tcgatatccg
     1201 caacggcacg cagctcgagt gcgtcaattg cacggcctgc atcgacgcct gcgatgaagt
     1261 gatggaaaaa gtgcacaagc cgaagggcct gatccgctat gcttccgaaa gcagcattgc
     1321 agataaaaaa caattgcgtt tcacgccgcg catcaaagct tatacattcc tgctgctgct
     1381 gctggtcggc gtggaagtgt tcctgcttgc cgggcgttcc gacgtcgatg cgacaatact
     1441 tcgtgccggc ggacaattat tccaggaaca gccgggcgac aagatcagca acctgtatac
     1501 cgtgcgcgtc atcaacaaaa caaggaacga ttacccgatc acgttccgcc tggaatccgg
     1561 ggacggggaa atacagatgg tcggaaaacc actgatggtg cagaaagaag gaaaaggcga
     1621 aacagaattt ttcatcctgc gggacaaaac ggatatccat tcccggaaaa caaagatccg
     1681 gatcggcgtt tattccggcg ataaaaagct ggaaagcgtt tccacgaatt tcctcggacc
     1741 cgtttatgtt cccggggaag atcaccacga agaagaagaa aaaaacgatc atcacgacta
     1801 aaactcaaaa acatgaactg gggcctgcgc attgttattc tctattccgc atttgtcgcg
     1861 cttatcctgt tcctggtcat ccagaccatg aagcagaaag tcgatcttgt ttccgacgat
     1921 tattaccaga aagagctcgt ttaccaggac cagatcgata aaatgaacaa cgcgaaagaa
     1981 ctttccgtgc agccggtcgt ttctgcggat gcacacgtcg tcaggatcac tttcccgcat
     2041 gccgacagcg ccgcaaatgt aaccggggtt attacttttt accggccttc cgattcttcg
     2101 aaggatctca cggaaaaaat aagcgccgga acagacggcg tgcaggtgct ttcatcggac
     2161 agtctcgaga aaggattgta ccagctccag atgaactggt cggttgccgg aaaaaattat
     2221 tacaacgaaa tccccgttta cattccctga tatgttcccc gtcgctgcct tgatacttgg
     2281 attgctcggc agcctgcatt gcgcgggcat gtgcggtccg ctcgtgctcg cgatgccgct
     2341 caaccggaaa aacagcgcaa cagctgcagc gggacttacg gtttataacc tgggcaaagc
     2401 gctttcctac gcgctgatcg gcgcggtttc cggtgcagcc gggagcgcgg tgaaatgggc
     2461 ggtgggacag caggtgcttt cggttgcagc aggaacattt attctcctcg tgctgctcgc
     2521 cggtcttttc ggtacgcggg tgaagctgcc gcgccaggtg gaaaaagtat tttcattcgt
     2581 gcggaacagc atcgggaaac ttttccggca gaaaggaaca cgcgccaatt ttctcatcgg
     2641 cgcaatgaac gggtttcttc cctgcggcct tgtttatgca gcagtggccg gggctgccgc
     2701 gagcgggagc tggtggaacg gagcggtttt catgttcgtg ttcgggatcg ggacgatgcc
     2761 tgcacttttt gcgctgggac ttgcaggttc gaagctgacc ttttcattcc gcgaaaagct
     2821 gcgtcgcgtg gttcctgttt ttgtcgctgt aatggccatg cttcttattc tccgcggact
     2881 cgggctggga attcctctcg tcagtccttc cctgcacgag ggaaaggttg tctgcccgca
     2941 ttgtcagcac aggcagaaat gataattatc atgttttctg cctgattgca ctcatgcatg
     3001 tcccgcaggt gcacggataa ttttgaggtc cgatcaatga actccagtct tatccgaaaa
     3061 tacgacgtag ccgctccgag atatacgagc tacccgactg tgccgtgctg gaatgcagga
     3121 aaaccttcgg aagagcagtg gcggatggcg gtaaaggaag ctgcgcagcg cgacggttcc
     3181 atcagcctgt acattcatct tccgttttgc gaaagcctgt gcacgtactg cgcgtgcaat
     3241 acgaggatca ccaaaaacca cgcggtggaa gaaccgtata ttgaaaatat tctccgcgaa
     3301 tggaaaatgt atgcggacat cctgccggaa gaggtggaaa tttcagagct ccatctcggc
     3361 ggcggaacac cgacattttt cagcccgcag cacctcgacc ggctgatgag cggattgaaa
     3421 aaggagatac gtttttctgc cggcgcatcg ctgagctttg aagcgcatcc tgccaatacg
     3481 acgacagcgc atctcgagac attgttccgt cacggtttcc ggagaatgag ccttggcgtg
     3541 caggatttca atccgacagt gcagaagctg atcaaccgga tgcagtcgga agcacaggtc
     3601 agctcggtta ccaaccttgc gcgcatcatc ggctatactt cggtgaactt cgatcttatt
     3661 tacggacttc cgaaacagac ggaagaatcc atcgcggaaa cgatcaaaac ggtgatcagg
     3721 atacgcccgg accgcatcgc gttctattcc tatgcgcatg ttccgtggat gaaacccggg
     3781 cagcgggcct attccgaagc ggatctcccg gaaggtgaaa agaaaagagc gttgtatgaa
     3841 aaaggaagag ctctcctgga aaagagcggg tacagtgaga tcggcatgga tcatttcgca
     3901 ttgccgggtg atgaattgct gcatgctgcg gtcagcggaa cgctgcaccg caatttcatg
     3961 ggctacaccg aaaaagccac gtcgctgctc atcggcctcg gcgtttctgc gatcagcgat
     4021 gcgggaacaa tgttcgtgca gaacgagaag accgtggaag catggagcct gcacatcggg
     4081 aaaaatgaaa tgcctttttt caaaggtcat gtgctcgatt acgaggacct cgtattgcgg
     4141 aaacatattt cagagatcat gtgcgcggga gaaacggcgt tttctgcgaa cggggaaccg
     4201 gccgcttttt acgaagggct ggaacgcctg aaggagctgg ttgcagacaa gctggtgcgg
     4261 gtaaaaaaca ataagatcac tgttacgaag agcgggaaag cattcctgcg gaatatatgc
     4321 atgtgcttcg atgcgcggct gcatcgcaac aggcccggcg acaaggtcta cagcaaatca
     4381 gcgtgaaatc atgaggggcc ccgttttatt ccatgcaggc tgtatattta cttttttatt
     4441 acgcttatga gccagctgga aatgcgcgat gccatgttcc gttatgcgac ggaaggaatt
     4501 ctcatctgtg ataacgatgg cgtcatccag ctgaccaacc cggccgtgcg caacatgttc
     4561 ggctacgagg aaaacgatct caaaggacag aaaatagaag ttcttcttcc acagcggcat
     4621 cgccgcgcgc acatcgggat gcgcacggat tattcacgcc ggcccgaacc ccggaagatg
     4681 ggctacgggc gcgatctttt cgccacgaaa aaggacggaa cggagatcat ggtggaagta
     4741 agcctttccc cgttc
//
LOCUS       DASTSF010000030         7640 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_867070,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000030 DASTSF010000000
VERSION     DASTSF010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 7640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7640
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_867070"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..700)
                     /locus_tag="VFU15_00860"
     CDS             complement(<1..700)
                     /locus_tag="VFU15_00860"
                     /inference="COORDINATES: protein motif:HMM:NF025749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4421 family protein"
                     /protein_id="HEU4716347.1"
                     /translation="MIRHPKFFLLLSAALFISSLHAQLFRSKRPGHLDSMMTFWNTQD
                     TNYIRKYPDRFIVTLSQSFREYDVGFSQTLVQDTAGWGAPDLVANANKSNSISVDFDK
                     ISFCLGLNTVPPTDDVLRKRGKTTYKTLTLSFGAYRFRFETGYRSYHGFYDKKTPAYD
                     SLFDSTGVYYQDPSMNVRSLRVKTIFIFNKRHFSYNSAYFNTQRQLKTSGSLLLCNNL
                     YDDRFSSDTSMIPRP"
     gene            complement(770..1630)
                     /locus_tag="VFU15_00865"
     CDS             complement(770..1630)
                     /locus_tag="VFU15_00865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716348.1"
                     /translation="MKRTVTRFFLASLLAGASLVSKAQAVIYHETFDADSSALPAGWS
                     SSGAWHPETSNTSNGYPGASGNVNITIRNDSATGTYDLVSKSVSTTGFSSVYVSWGAR
                     LTTNFPSGGSSIQSLSWSTDGGLTWTPVAYTENSNNSNWSLDNGGMPVVLPSGAGNQS
                     SLKIKFTAAIVNDSQGTYRIDDFTVGDASSIGIREFPSSVFASATAVPNPSSSGPVHL
                     SVNSSENCTAVVRVCDILGRGTGSGMTVVLANGVNDILLDETLFPSAGIYFVWISSGR
                     NTAYARVTRN"
     gene            complement(1655..2806)
                     /locus_tag="VFU15_00870"
     CDS             complement(1655..2806)
                     /locus_tag="VFU15_00870"
                     /inference="COORDINATES: protein motif:HMM:NF019042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEU4716349.1"
                     /translation="MKINIVTRLFVFLLLMKAAAVNAQFGIVKGNSFIEFSGAIVTDY
                     NYRFYPAAKTDHHKNGFTPDVMEFQLKGVYAKNFRYCLDVNFANLIPADTTRLDYYVD
                     PALIREAYVSYSGLRRFADIKVGYMKLPFSRSSITEFAESPFLQRGEIARGDVFSRRD
                     IGIQLDRELMNRRLFIRAGAYTGLGEGSMGGDNDASGKLLYVGRIDYSWPGRYRFSEY
                     DLSVTPLPVFSVGVNGMYEEKESDAGVDYGIQTINGKKSGYGFNADAKFMGFTAHFEA
                     LQFRMVPNDTLRLLGKPTDYFLAGGIIASVNYYCKCLHSVFAVRYDELNPDDLTLHDT
                     ERTISFAYNYLISQENACVKIQYWYHLDNDLTHEPWTPNQLRVGFQLLF"
     gene            complement(2803..3522)
                     /locus_tag="VFU15_00875"
     CDS             complement(2803..3522)
                     /locus_tag="VFU15_00875"
                     /inference="COORDINATES: protein motif:HMM:NF018659.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdiA-regulated domain-containing protein"
                     /protein_id="HEU4716350.1"
                     /translation="MKNHLLLTALVITCCSASHHASKKLKPTTSYKLKMTEPSDVCIS
                     GDGKSLYIASGDRYIYEVDFTGKIIRKGAYEGFDYEAVYADENYVYANDESLRRIDVY
                     DPKTLNVVKSYDLDYNGPINKGFEALTWNGTKNCFVLVSEKNPCVLYEYDKDFRKIDE
                     INVPQFREISSATYYNGNLWLQSDEEETLYKLNPQDYSVTDQWRVPVVNPEGIAFDRE
                     GNLFMSSDDMGKLFYFSKPQL"
     gene            complement(3534..4127)
                     /locus_tag="VFU15_00880"
     CDS             complement(3534..4127)
                     /locus_tag="VFU15_00880"
                     /inference="COORDINATES: protein motif:HMM:NF013125.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HEU4716351.1"
                     /translation="MKCSVFILLLLFLGGCTKDRIFPVPETPLTQQDTLSVITGTSGQ
                     HMILHPGDLLINEFVATGSTYPNELGSPSDWVELYNSRTDTVIMYSGYWYTTDTLGEP
                     GKYPFPVTIKIPPHSYYVYQCDGNDSYVTQVHTNFKLSASGEQIGLYYSSDGNSFRAI
                     DSYTFSAQTAGLSTGRIPDGSTTWNTLAPTPGTSNHQ"
     gene            4499..>7640
                     /locus_tag="VFU15_00885"
     CDS             4499..>7640
                     /locus_tag="VFU15_00885"
                     /inference="COORDINATES: protein motif:HMM:NF024962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716352.1"
                     /translation="MATKKIFLFASLFAAAFSFPLHAQFTTGNVVVLQVGDGTEALSN
                     AGNSLFLKEFTPAGGNGISVTIPDNGAGALLNSGTATSEGQITLSSDGKLILIPGYNT
                     AYPFPSPIASSSSATVPRAIGQVDGFGTYSFVASSPSFFSGNNIRSATGDGNNNYWAS
                     GANSGLCYYATGTPAAVSTTVNNERVAHLCNGNLMFSTGSGTPSVYTLPGAPTAGPVI
                     ASIAVSIAGGSPYGFAVDPGNTTLYIADDRTFSSTAAQAGGIEKYTWNGSAWIFQYTL
                     QCGAGTFGARGLTVDFSGANPVIYATTTETTSNRLISITDVGASSGSSALVLSTAPAG
                     TVYRGVVFSPCAPVSISSLSTNSPVCEGATLHLSSSTGGSPAYTYSWSGPNSFSSGQQ
                     NPAITNAHISSGGTYTLTVTNACGTSTQNISVTIIPSPSVTLTSSTDELCNGDSNGTA
                     AVSASGGNPGYTYNWTPSGGTNASASGLGAGTYTCTVSDMSNCSSTQTVTITEPAALS
                     ASIISSSDVTCNGSNDGQASVSVTGGTSAYSYAWTPSGGNAAGASGLSPNNYTCNITD
                     ANGCTTSASVVITEPVALSVAFSQTNNTCANANDGSATVSPGGGTGPYTYSWSSGGTN
                     ATETNLAAGSYTCTITDAHSCTITQSFTITQPSALTSSVTAVTNVTCNGNGDGSATVS
                     ASGGTSSYSYAWTPSGGNTATASSLPVGNYTCTVTDANGCTTTSSVSITQPSTLASSL
                     SSQANVSCNGGSDGTATVAVSGGAPSYNYSWMPSGGNSATAPSLAQGSYTCTITDANG
                     CSVTTSVSITEPAALTVTNAQNNVSCNGGNNGAASVSVNGGTPSYTYAWLPSGGSSDT
                     ASSLPQGNYTCTITDANGCTVTSSVSITEPAALSSSVTATTNVSCNSGNDGAATVSAN
                     GGTPSYSYSWSPSGGNAAAATSLPQGNYTCTITDANGCSTTASLSITEPAALTSSVTS
                     TTNVSCNSGNNGSATIAAGGGTPSYSYAWTPSGGSAATAASLSQGNYTCTITDANGCT
                     ATASVSITQPSAITTSV"
BASE COUNT         1772 a         2138 c         1971 g         1759 t
ORIGIN      
        1 acggccgcgg gatcatcgac gtgtccgaag aaaaacgatc gtcgtacagg ttgttgcaca
       61 agagcagcga gcccgatgtt ttcaactggc gctgcgtatt gaaataggcc gaattgtacg
      121 agaaatgacg cttattgaaa atgaagatcg ttttcacacg cagcgaccgc acgttcatcg
      181 acgggtcctg gtaatacacg ccggtggaat cgaacagcga atcgtacgcg ggtgtttttt
      241 tgtcgtaaaa gccgtggtaa ctccggtagc cggtctcaaa acggaaacgg tatgcgccga
      301 aggaaagcgt cagcgttttg tacgtcgttt tcccgcgttt gcggagcaca tcgtcggtcg
      361 gcggaacggt gttgaggccg aggcagaaag atatcttgtc aaaatcgacg gagatgctgt
      421 tcgacttgtt cgcgttggca accaggtcgg gtgcgcccca tccggccgtg tcctgcacga
      481 gcgtctgcga aaaccccacg tcgtattcac ggaacgattg cgagagcgtg acaataaaac
      541 ggtcgggata tttccggatg taattcgtgt cctgcgtatt ccagaacgtc atcatcgaat
      601 cgaggtggcc gggacgtttc gaacggaaca gctgcgcatg aagggaggaa ataaaaagtg
      661 ccgcggaaag cagcaggaaa aatttcggat ggcggatcat atccggggaa ttgtgatttc
      721 cgaagatacg atttctccaa tgaaaaaata aagccgcagc tggttgtcgt cagttcctcg
      781 tcaccctggc gtacgccgta tttctgccgg aactgatcca cacaaaataa attccggcag
      841 agggaaacaa tgtttcatcc aggagaatgt cgttcacacc gtttgctaat acaactgtca
      901 tcccgcttcc tgtcccgcgc ccgagaatgt cgcacacgcg caccacagcc gtgcagtttt
      961 ccgaagaatt caccgacaaa tgaacaggtc cggatgaaga aggattggga acagcggttg
     1021 cggatgcaaa tacggaagac ggaaattcgc ggataccgat ggaagaagcg tcacccacgg
     1081 taaaatcatc gatccggtaa gttccctgcg aatcgttcac gatcgccgca gtgaatttga
     1141 tcttcagcga agactggttg ccggctcctg aaggaagcac gacaggcatg ccgccgttat
     1201 cgagcgacca gttgctgttg tttgaatttt cggtgtacgc aacgggcgtc cacgtcaatc
     1261 ccccgtcggt gctccacgac aagctttgaa tggagcttcc gccggacgga aaattcgtcg
     1321 tcagccgcgc gccccagctg acatagacgg aactgaatcc cgtcgtggaa acgcttttcg
     1381 acaccaggtc atacgttccc gttgcgctgt cgttgcgtat ggttatgttg acgttcccgc
     1441 tggcgccggg ataaccgttt gaagtgttcg aggtttccgg gtgccatgca ccggatgagc
     1501 tccatcctgc gggaagcgcc gaagagtccg catcgaacgt ttcatggtaa ataacagcct
     1561 gggcttttga gaccagcgaa gcgccggcca gaagtgaagc gagaaaaaag cgggttactg
     1621 ttcttttcat cctggtggtt ttgaatttta tttttcagaa gagcagctgg aatccgacgc
     1681 gcagctggtt gggcgtccac ggttcgtgcg tgagatcatt atcgagatgg taccagtact
     1741 ggatcttcac acaggcattc tcctggctga tgaggtaatt gtatgcgaac gagatcgtac
     1801 gttccgtatc gtgcagcgtc agatcatccg gattcagttc gtcgtagcgc acggcaaaaa
     1861 cggaatgcag gcatttgcag tagtaattca cgctggcgat aattcccccg gcgagaaaat
     1921 aatccgtcgg cttgccgaga aggcgcagcg tatcgttcgg gaccatccgg aactggagcg
     1981 cttcgaaatg cgctgtgaat cccatgaatt tcgcgtcggc gttgaaacca tatccggatt
     2041 tttttccatt gatcgtctgg atgccataat caacgcccgc atccgattct ttctcttcat
     2101 acatgccgtt cacgccaacg gaaaaaacag gaagaggcgt aacgctcagg tcatattcgg
     2161 aaaaacggta acgccccggc caggaataat cgatcctgcc gacataaagc aatttgccgc
     2221 tggcgtcatt gtcgccgccc atgcttcctt caccgagccc ggtgtacgca ccggcgcgaa
     2281 tgaaaagtct ccggttcatc agctcgcggt cgagctgtat gccgatatcg cggcggctga
     2341 aaacatcgcc ccgcgcaatt tctccgcgct gcaggaacgg cgattcggca aattccgtga
     2401 tcgaggaacg cgagaaaggc agtttcatat agcccacttt tatgtcggcg aatcgccgga
     2461 ggcccgaata cgaaacgtac gcttcgcgga tcagcgccgg gtcgacgtaa taatcgaggc
     2521 gcgtcgtatc ggccgggatg aggttcgcga aattgacgtc gaggcagtac ctgaaatttt
     2581 tcgcgtacac acctttcaac tggaactcca tgacatccgg cgtgaaaccg ttcttgtgat
     2641 gatcggtttt tgcagccggg taaaaccggt aattataatc ggtgacgatc gctcctgaaa
     2701 actcgatgaa gctgttccct tttacaatgc cgaactgcgc attcactgcc gcagctttca
     2761 tcagcagcag gaaaacaaac aggcgcgtga cgatatttat tttcatagct gtggtttgct
     2821 gaaataaaag agcttgccca tgtcgtcact cgacatgaaa agatttccct cccggtcaaa
     2881 cgcaattcct tccgggttga caaccggcac gcgccactgg tcggtgaccg agtaatcctg
     2941 cgggttcagc ttgtacagcg tttcctcttc gtcgctttgc agccagagat tcccgttgta
     3001 ataagtggcg gaagaaattt cccggaactg cggcacattg atctcgtcga tcttccggaa
     3061 atccttgtcg tactcgtaca gcacgcaggg atttttttca ctgacaagga caaaacaatt
     3121 cttcgttccg ttccacgtca gcgcttcgaa tcctttgttg atcggtccgt tgtaatcgag
     3181 atcatacgac ttcaccacgt tcagcgtttt cggatcgtac acgtcgatcc tgcgaaggct
     3241 ttcatcattt gcatacacat aattttcatc tgcataaaca gcttcgtagt cgaatccttc
     3301 atacgctcct ttgcggatga tcttcccggt aaaatcaact tcatagatgt aacggtcccc
     3361 gctcgcgatg tacaatgatt ttccgtcgcc ggaaatgcac acgtcggaag gttccgtcat
     3421 tttcagcttg tacgacgttg tgggtttgag ctttttcgaa gcgtgatgcg acgcgctgca
     3481 gcaggtgatc acgagtgcgg tcagcagtaa atgatttttc atacgttgcg ttttcactgg
     3541 tgattggacg ttccgggagt gggcgccagc gtgttccacg ttgtgctgcc gtcggggatg
     3601 cggcccgtgg aaagtcctgc agtctgcgcg ctgaaggtgt agctgtcgat agccctgaac
     3661 gaattcccgt cggaagaata gtaaagaccg atctgctcgc cggaggcact gagcttgaaa
     3721 ttcgtgtgca cctgcgtgac gtaagaatca ttgccgtcgc attggtacac gtagtaagaa
     3781 tgcggcggaa tttttatcgt tacgggaaac gggtatttgc ccggctcccc gagcgtgtct
     3841 gtcgtgtacc agtacccgct gtacatgatc accgtatccg ttctcgaatt gtaaagctcg
     3901 acccagtcgg acggagaacc cagctcattc gggtatgtgc tgccggtcgc tacgaattca
     3961 ttgatgagca gatcgcccgg gtgaaggatc atgtgctgcc cggatgttcc cgtgatcacc
     4021 gataacgtgt cttgttgcgt gagtggtgtt tccggaacag ggaagatcct gtctttcgta
     4081 cacccgccga gaaacagcag gaggagaata aatacgctgc acttcatttt ttgcattgtg
     4141 caaacgtagc tgcgcaacgt taactaattc tccaccgtga tatatgtgat agttaattaa
     4201 ttcttagaac gaatttcatt taactgtctt tatatactaa tacacatccg gcagtccatt
     4261 cattaaaata ttcttatttc ccggttcgct tttttctttc ttttttttca acgtgcccag
     4321 catcagaata ttccgcgcac aacttttcga tttgtaaaca aaagcgattt cccattaaca
     4381 gaagtataac aataaacacc gctttgagga aaagccggga tttagttttg ccgcgtcgat
     4441 cctttttgct tcagcttttt cgcaccgcgt aatctctttt cgaaagaatc cgtaacaaat
     4501 ggcaacaaaa aaaatctttc tttttgcttc actgtttgca gctgcatttt cattcccgct
     4561 gcacgcgcag ttcacgacgg ggaacgtggt cgtgctgcag gtgggcgacg gtaccgaagc
     4621 gctgagcaat gccggcaaca gtcttttcct gaaagaattc acgccggcgg gaggcaacgg
     4681 catctctgtc actatccccg ataacggggc cggtgcattg ctcaacagcg gaaccgcaac
     4741 atccgaaggt cagatcacgt tatcttccga cggaaagctg atccttattc ccggttacaa
     4801 cacggcttac cctttcccct ccccgatcgc cagctcgtcc tctgccaccg tcccgcgcgc
     4861 gatcggccag gtggatggtt ttggcaccta ttcatttgtc gcatccagcc cgtcgttctt
     4921 cagcgggaac aacatacgca gcgcgaccgg cgacggaaac aataattact gggcgagcgg
     4981 ggccaactcc gggttgtgtt attatgcaac cggaacaccg gccgccgtca gcacgacagt
     5041 caacaacgaa cgggtcgcgc acctctgcaa tggcaacctc atgttctcca caggctccgg
     5101 cacgccttcg gtctatacgt tgcccggcgc acctacggcc ggccccgtca tcgcttcgat
     5161 cgccgtttcc attgccgggg gaagtcctta cggttttgct gtcgatcccg gcaacacaac
     5221 attgtacatc gccgatgacc gtacgttctc ttctacggca gcacaggcag gcggaattga
     5281 aaaatacacc tggaacggat ccgcctggat tttccagtac acgctgcaat gcggagcggg
     5341 aacgttcggc gcacgcggac tcaccgtcga tttcagcggt gcgaatcctg tgatctacgc
     5401 aacaacaacg gaaacaacgt ccaaccggct gatcagcatt acggatgtgg gtgcatcttc
     5461 gggcagctcg gcgctcgtac tttccacggc accggccgga accgtttatc gcggtgttgt
     5521 cttttcaccc tgcgcgccgg tttccatttc atcgctgagt acaaattctc cggtatgcga
     5581 aggcgcgaca ctccatttgt cgtcttccac cggcggttct cccgcttata catattcctg
     5641 gagcggaccg aacagcttca gttccggtca acaaaatccc gcgatcacca atgcacacat
     5701 ttcgtcagga ggaacttata cgctgacggt tacaaatgcg tgcggcacga gcacacagaa
     5761 tatttccgtg accatcattc catcaccttc agtcacgctc acttcttcca ccgatgagct
     5821 gtgcaacggc gacagcaacg ggaccgcggc cgtttccgca agcgggggaa atcccggtta
     5881 cacatacaac tggacgccat caggcggaac gaatgcttct gcatccggcc tcggcgcagg
     5941 aacatacacc tgcaccgttt cggacatgag caattgttca tcgacgcaaa cggtaacgat
     6001 caccgaaccc gctgcgctca gcgcaagcat tatctcgtct tcagacgtaa cctgcaatgg
     6061 cagcaatgac gggcaagctt ccgtctcagt gaccggtgga acttccgcgt attcctatgc
     6121 atggacgccg tccggtggaa atgcagcggg cgcgtccgga ctttctccga acaactacac
     6181 ctgcaacatc accgatgcga atggctgcac gaccagcgct tcggttgtca tcacggagcc
     6241 cgtggcgctt tccgttgctt tttcgcagac caacaacacg tgcgccaacg cgaacgatgg
     6301 ttccgctacc gtttcaccgg gcggcggaac cgggccgtat acgtattcat ggagctccgg
     6361 cggaacgaat gcgacggaga cgaacctcgc cgccggcagt tacacgtgca ccatcacgga
     6421 tgcgcatagc tgcacgatca cacaatcatt cacgatcacg caaccttccg cgctcacctc
     6481 ttccgtcaca gcggtaacca acgtcacctg caacggcaac ggggacggtt ccgcaacagt
     6541 ttccgcgagc gggggaactt cctcctattc gtatgcgtgg acaccgtccg gcggaaatac
     6601 ggcaacagcc tcttcactgc ccgtcgggaa ttacacctgc acggtcaccg atgcgaacgg
     6661 gtgcacgacc acttcttccg tttcgatcac gcagccgtcg acgctcgctt cttcgctttc
     6721 ttcacaggca aatgtttcgt gcaatggcgg cagcgacgga acagcaaccg tagcggtaag
     6781 cggcggagcg ccatcctaca attattcgtg gatgccttcg ggaggaaatt cggctacagc
     6841 accttcgctt gcacagggaa gctatacgtg cacgatcacc gatgcaaacg gatgttctgt
     6901 tacgacatcg gtaagcatta cagaaccggc agcgctcacc gtaacgaatg cgcagaacaa
     6961 tgtttcctgc aacggcggga acaacggggc cgcaagtgtt tccgtgaacg ggggaacgcc
     7021 ttcctatacg tatgcatggc ttccttccgg cggcagcagc gacaccgcat cttctcttcc
     7081 gcagggaaat tacacctgca cgatcaccga tgcgaacgga tgcaccgtaa catcctccgt
     7141 ttccattacc gaaccggccg cactttcttc ttccgtcacg gcaactacaa atgtttcctg
     7201 caacagcggg aatgacgggg cagcaacagt ctccgcgaac gggggaacac cgtcctattc
     7261 gtattcatgg tcgccttccg gtggaaatgc agcggcagcc acttcactcc cgcagggaaa
     7321 ttacacctgc accatcaccg atgcgaacgg atgcagcaca accgcctcac tgtcgatcac
     7381 ggaaccagcg gcgctcacat cttccgtcac ttcaaccacg aacgtttcct gcaacagcgg
     7441 gaacaacgga tctgcaacaa tcgctgcggg aggaggaacg ccttcttact cgtacgcatg
     7501 gacaccctcc ggcggaagtg cagcgacagc cgcttccctt tcgcagggaa attacacctg
     7561 cacgatcacc gacgccaatg gatgtacggc aacagcatcc gtttccatca cacaaccgtc
     7621 cgccatcacc acttccgtct
//
LOCUS       DASTSF010000031         2218 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1313624,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000031 DASTSF010000000
VERSION     DASTSF010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1313624"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1646
                     /gene="lepB"
                     /locus_tag="VFU15_00890"
     CDS             <1..1646
                     /gene="lepB"
                     /locus_tag="VFU15_00890"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEU4716353.1"
                     /translation="KAFEKAGEKGWKALVPVYNFVIWLRIIKRPWWWVLLVIIPTVGF
                     YMLAVMIVLLSNCFGKRKFTDHVIAVLGYFIWLPYLGFSKEVKFTGVPPAPDKKPILK
                     EWGEAAIFAVVAATLIRTFFFEAFVIPSSSMESTLLTGDYLFVSKMSYGAKVPAIPLT
                     FPFTHNTMPFSETMPSYLDWIELPEMRLPALGHVQRNDIVVFNFPEGDTVWTGNRNES
                     YYGHLRREAERTGRSTDDIRKQIVGDEINVNPVDKEDNYVKRCVAVPGDVIEIKDKHL
                     YVNNGLAFVAKGMQWAQDFNTPIPILEQEQDGSWRMIEERYAQVHDQLGQAGITDDFG
                     SSRADSTYQFSVPVNSAKMEVFNQIVGKSRTSLLPKGFIDTDVFPHDTAHYKWNRDNF
                     GPLTIPKKGMTVKLAPESIAPYERIIKVYEGNTLEYKDGSYYVNGQKTDTYTFKQDYY
                     WMMGDNRDNSLDSRFWGFVPDDHIVGKPVFVWMSKGTYSGLRPERMMAFVSPEGLSKS
                     YLWYVIIGIGLIVGYSQFFGKKKRPGNDAKAKKAVVKKK"
     gene            1685..>2218
                     /gene="lepB"
                     /locus_tag="VFU15_00895"
     CDS             1685..>2218
                     /gene="lepB"
                     /locus_tag="VFU15_00895"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEU4716354.1"
                     /translation="MEEKDLREQDEEHSQKKTSADSSELVPSVDDPSADYDSGEKKNE
                     PVAEKKQEAKPPKKKSHIREWVEALVFAFAGVLILKTFVFEPFAIPSDSMDATLETGD
                     YIVVNKLAYGPRLPMTPLTIPFTHQTIGGMKSYLEWFSFPYMRLPGYSSVKRNDVLVF
                     NFPAEDLFPLNGKPVNYP"
BASE COUNT          563 a          555 c          586 g          514 t
ORIGIN      
        1 acaaagcatt tgaaaaagca ggagagaaag gatggaaagc gctcgttcct gtttacaatt
       61 ttgttatctg gctgcggatc ataaaacggc cgtggtggtg ggtgctgctg gtgatcattc
      121 ccacggttgg attttacatg ctggccgtga tgatcgtcct gctttcgaat tgcttcggca
      181 aacggaaatt cacagatcac gtcattgcag ttctcggcta tttcatctgg cttccttatc
      241 tcggcttttc gaaagaagtg aaattcactg gcgttccccc ggcgccggat aaaaaaccga
      301 tactgaagga atggggagag gccgccattt ttgcggttgt cgcggcgacg cttattcgca
      361 cgttcttctt cgaggctttc gtcattcctt cgtcttccat ggaaagcacg ctgcttaccg
      421 gcgattatct tttcgtgagc aagatgagct acggcgcgaa agtgcccgcc attccgctga
      481 cattcccgtt cacgcacaac acgatgccgt tttcggaaac gatgccgtcg tacctcgact
      541 ggatcgaatt gccggaaatg cgtttgcccg cactgggtca cgtgcagcgc aacgacattg
      601 ttgtgtttaa tttcccggaa ggcgataccg tgtggaccgg taaccgtaat gaaagctatt
      661 acggtcattt aaggcgcgag gcggaacgga ccggaagatc gaccgacgat atacggaaac
      721 agatcgtcgg ggatgagatc aatgtcaacc cggttgacaa ggaagacaac tatgtcaagc
      781 gttgtgttgc tgttcccggc gatgtgatcg agatcaaaga caagcacctg tatgtgaaca
      841 acgggttggc tttcgtagcg aaaggaatgc agtgggcgca ggatttcaat acgccgatcc
      901 cgatcctcga acaggaacag gatggaagct ggaggatgat tgaagaacgt tatgcgcagg
      961 ttcacgacca gctcgggcag gcgggaataa cggatgattt cggatcatcg cgtgcggaca
     1021 gcacgtacca gttctctgtt cctgtcaaca gcgcgaagat ggaggtcttc aaccagatcg
     1081 tcggtaagtc gcgcacgtcg ctcctgccga aaggatttat cgacacggat gttttcccgc
     1141 acgatacggc ccattacaaa tggaaccgcg acaatttcgg tccgctgacg atcccgaaaa
     1201 aaggaatgac ggtgaagctt gcaccggaat ccatcgcccc gtatgaacgc atcattaaag
     1261 tgtacgaagg gaacacgctc gaatacaaag acggaagcta ttacgtcaac ggacaaaaaa
     1321 cggacacgta cacgttcaaa caggattatt actggatgat gggtgacaac cgcgacaatt
     1381 cgctcgattc ccgtttctgg ggttttgttc cggatgatca tatcgtcggg aaacctgtgt
     1441 tcgtgtggat gtccaaaggc acgtacagcg gtctgcgccc ggagcgcatg atggcatttg
     1501 tttctcctga aggactttca aagtcgtacc tttggtacgt cattatcggc atcggcctta
     1561 tcgtcggtta cagccagttt ttcggaaaga aaaaacgtcc gggaaatgac gcaaaagcga
     1621 aaaaagctgt cgtgaagaag aaataatcac ttcgttcttc gcgtccccgc caggaaaaca
     1681 agccatggaa gaaaaagatc tccgggaaca ggacgaagag cattcacaga aaaagacttc
     1741 tgcggattcc tccgaacttg ttccatccgt ggacgatcct tccgccgact acgattccgg
     1801 tgaaaagaag aacgagccgg tcgcggaaaa aaagcaggaa gcaaaacctc cgaagaaaaa
     1861 atcgcatatc cgcgaatggg tggaagccct cgtgtttgca tttgccggcg ttctcatttt
     1921 aaagacgttc gttttcgagc ctttcgccat tcccagtgat tccatggacg ccacgctgga
     1981 aaccggcgat tatatcgtcg tgaacaagct cgcgtacgga ccgcgcctgc ccatgacgcc
     2041 gctcaccatc cccttcacgc accagacgat cggcggaatg aaatcgtacc tcgaatggtt
     2101 ttcgttcccg tacatgcgcc ttcccggtta ttcctcggtg aagcgcaatg atgtgctcgt
     2161 gttcaatttt ccggcggaag accttttccc gctcaatgga aaaccggtga attatccc
//
LOCUS       DASTSF010000032         4558 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_418029,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000032 DASTSF010000000
VERSION     DASTSF010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4558
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_418029"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..662
                     /locus_tag="VFU15_00900"
     CDS             <1..662
                     /locus_tag="VFU15_00900"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU4716355.1"
                     /translation="KTLMKYITEKDHVADFGCGVGGFLPTLSSLGKTVTGIDFSAKCI
                     DIAKKHLKKFPNIRLVVHDMTKPLRLKCDVAVAANFLLSRDAALLEKMLKNMLAPVKK
                     GGHAIIVTPALESMLLVYRKIAELRRKHGESHTRARSYVEKQMKKDIVSLADGVLLVG
                     GVPTKHYLGEEMEAQLQKHGFGLVERYKIEYSWDTELDNISRNEKGPYPWDWLFVGKK
                     K"
     gene            1105..1980
                     /locus_tag="VFU15_00905"
     CDS             1105..1980
                     /locus_tag="VFU15_00905"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEU4716356.1"
                     /translation="MKSLPFKIPVPGENSVIVEEEIEPHFYNFLHQHQEIQLTWILKG
                     EGTLLVGNFTGQFGSGDIYWFGPNLPHVFRCDPVYFQNKNWLKAQSISVYFDISDARN
                     GLLSLPELETLKKFAARSIRGFRIEPEDYVAMTEAFLRIRVQSGLPRLTAFLQLMDQV
                     LHHSKHIPLSDGPVGHELTEKEGTRMHSIYDFTLKNFDKEVSVGDAAEVVHMTPEAFC
                     RFFKKHTRKTYVTFLNEVRVEHACRKLKTSDDKLVAEIAYSCGFGNVSHFNRVFRKVT
                     GKTPKEYQSQAAKKK"
     gene            complement(2036..2599)
                     /gene="pth"
                     /locus_tag="VFU15_00910"
     CDS             complement(2036..2599)
                     /gene="pth"
                     /locus_tag="VFU15_00910"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015546856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEU4716357.1"
                     /translation="MKYLIAGLGNIGDEYDGTRHNIGFAVIDRLAKDAGISFRHDRHA
                     FAAEYKFKGRIFILIKPTTYMNLSGKAVQYWLQAEKIPVENLLVITDDIALPFGTIRI
                     KAKGSDGGHNGLKSIQETLGRTDYARLRFGVGNDFPKGRQVEYVLGKWSAEQEAALSE
                     RLEKCGEAIRAFGTIGIGLTMTNFNGK"
     gene            complement(2699..3346)
                     /locus_tag="VFU15_00915"
     CDS             complement(2699..3346)
                     /locus_tag="VFU15_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013668341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HEU4716358.1"
                     /translation="MKSVAMSGSPRANVGKKDAKELRRNGMVPCVLYGGPEQVAFSVE
                     LSAFKTLVYTPEVNTIDLEIGGKAHKAILKELQLHPVTDRIIHADFIEIVPGKNVTVE
                     LPVKFEGTAPGVRAGGKLIRKMRKLKVNADVSKMPESIVLNVDKLEIGDAIRVGDVEV
                     DGLEFLDDENVTIVTVRVTRNVVEEEKPAAATAAAPAAAAAPAAAAKPAADAKKK"
     gene            complement(3385..4317)
                     /locus_tag="VFU15_00920"
     CDS             complement(3385..4317)
                     /locus_tag="VFU15_00920"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HEU4716359.1"
                     /translation="MAEHVKIFSGSVSHYIADKIAAAYGENLGQVTLHKFSDGEFQTS
                     LEETVRGATVFFVQSTFPPTDNLFELLLMVDAAKRASAKEIIAVIPYFGFARQDRKDQ
                     PRVAIGAKLVADMLATAGVNRVMTMDLHADQIQGFFNVPVDHLFASTIFIPYIEKLKL
                     KNLTMAAPDMGGSKRANAYAKHLKCDIVICYKQRSKANVVDHITAIGDIEGKDIVLLD
                     DIVDTGGTLCKAADMMMERGATSVRAFITHPVLSGKAYENIEKSQITELIVTDTIPLK
                     RESPKIKVLSVAEMFADVFHRVVNYESISSHFIM"
BASE COUNT         1041 a         1199 c         1211 g         1107 t
ORIGIN      
        1 gcaagacgct gatgaaatac atcacggaaa aagatcacgt cgccgatttc ggctgcggtg
       61 tcggaggatt tcttcccacg ctttcttcat tggggaaaac ggtcaccggc atcgattttt
      121 ctgcaaaatg catcgacatc gcgaaaaaac acctgaaaaa gttcccgaat atccgcctgg
      181 tggtgcacga catgacgaaa ccgttgcggc ttaaatgcga cgtggccgta gcagcgaatt
      241 ttttactttc ccgcgatgcg gcgcttctcg aaaaaatgct gaaaaacatg ctcgcgcccg
      301 tgaagaaagg cggtcatgcg atcatcgtca cgcccgcgct ggaatccatg ctgctcgttt
      361 accgcaagat cgcagagctg cggagaaaac acggagagtc gcacacacgc gcgcgcagct
      421 acgtggaaaa gcagatgaag aaagacattg tttcactcgc ggacggcgtg ctgcttgtgg
      481 gcggcgttcc gacgaaacat tacctcggcg aggaaatgga agcgcagctg cagaaacacg
      541 gtttcggact cgttgagcgt tacaagatcg aatattcctg ggatacagag ctcgacaata
      601 tttcccgcaa tgaaaaagga ccgtacccgt gggactggtt gttcgtgggg aagaagaaat
      661 gaacgtttcg ggttttcggt ttcagcggta aatgaaaaca gcaccgcagg accggtttca
      721 acgcgtttgc cgggtatgtt ttttcttctc cgtgccggaa ttacccggca gcacttcgcc
      781 cgggtgaatg ttccggagtt ttttatttcg cggaatgttt atgaaaagaa aaccccgccg
      841 tgcaaaaaac aacaaaggcc ggtttcccct tccggccttt gtctttggtg tgttgaacgg
      901 aagcgtattt gcaaacccgc ttcagctctc tgcaaaatcc ccaaaaccca tttttggaat
      961 tcgcagcggt acaaagctat atttcatcct tgataaaccc taaaccgatt ttagctgaga
     1021 atttgctaat tttgatctga aacaatatcg aactgtttct tcgcgattaa tatataacca
     1081 actttacagg aaggtttacc gataatgaaa tcgctaccgt tcaagattcc cgttcccggc
     1141 gaaaattccg tcatcgttga agaggaaata gaaccgcatt tctacaactt cctccaccag
     1201 caccaggaga tccagcttac gtggatcctc aaaggcgaag gaacgctgct cgtgggaaat
     1261 ttcacgggac aattcggctc cggcgacatt tactggtttg gtccgaacct gccgcacgtg
     1321 ttccgctgcg acccggttta tttccagaac aagaactggc tgaaagcgca atcaatatct
     1381 gtttacttcg acatttccga cgcgcgcaac ggcctgcttt cactgccgga gctcgagacg
     1441 ctgaaaaaat tcgccgcgcg ttccatccgc ggcttccgca tcgagccgga agattatgtc
     1501 gccatgactg aagcgttcct gcgcatacgc gtgcagagcg ggcttccgcg cctcacggcg
     1561 ttcctgcagc tcatggacca ggtgctgcac cacagcaagc acatcccgct ttccgacggt
     1621 cctgtaggcc acgagctgac ggaaaaagaa ggaacccgca tgcattcgat ctacgatttc
     1681 acgctcaaga atttcgacaa ggaagtgagt gtgggcgatg cggcggaagt ggtgcacatg
     1741 acgcccgaag cgttctgccg ttttttcaaa aagcacacgc gtaaaacgta cgtcacattc
     1801 ctcaacgaag tgcgcgtgga gcatgcctgc cggaaattga aaacgtcgga cgacaagctc
     1861 gtcgcggaga tcgcgtattc gtgcggcttc gggaacgttt cgcatttcaa ccgtgtgttc
     1921 cgcaaagtga cgggaaaaac gccgaaggaa taccagtcgc aggcggcgaa gaaaaaatag
     1981 ggagttgttt agcgtgcgct ttttgcgcaa cgccgttctc tgaaagcgaa aaaaattatt
     2041 tcccgttgaa attcgtcatc gtcagcccga tgccgatcgt gccgaaagcg cgtatcgctt
     2101 ccccgcattt ttccaggcgt tcgctcaatg cggcttcctg ttctgccgac catttcccga
     2161 ggacatattc cacctgtctt cccttcggaa aatcattgcc gacgccgaaa cgcagccgcg
     2221 cataatctgt tcttcccaat gtttcctgta tgctttttaa gccgttgtgt ccgccgtcgc
     2281 tgcctttcgc tttgatgcgg atcgttccga agggcagagc gatgtcgtcg gtgatgacga
     2341 gtaaattctc aaccgggatc ttttccgcct gcagccagta ctgcacggct ttcccgctca
     2401 ggttcatgta cgtcgtcggc ttgatgagaa tgaaaatgcg tcctttgaat ttgtattccg
     2461 ctgcgaacgc gtgacggtcg tgacggaaag agatccccgc atctttcgcg aggcggtcga
     2521 tgacggcgaa gccgatgttg tggcgcgttc cgtcgtattc gtccccgatg ttgccgagtc
     2581 ctgcgatgag gtatttcata agcgctttta aaaagctggt gatgcgatct gctgcggaaa
     2641 aatagaaaaa ccgcggaaat tattctccgc ggttttcgtt ctgtgctgaa aaaggaaatt
     2701 actttttctt cgcgtctgcg gccggtttcg ctgctgccgc cggagctgcc gctgctgcgg
     2761 gtgctgctgc cgtggctgct gcgggcttct cttcctctac aacgttgcgc gttacgcgga
     2821 cggtaacgat cgttacgttt tcatcatcga ggaattccag tccgtcgact tcaacatcgc
     2881 cgacccggat tgcgtcgccg atctcgagct tgtcgacatt cagcacgatg ctttccggca
     2941 ttttgctgac gtcggcattc accttcagct tgcgcatttt gcggatgagc tttccgccgg
     3001 cgcgtacacc gggtgctgtg ccttcgaact tgaccggcag ttccaccgtc acgtttttac
     3061 cgggaacgat ctcgataaaa tcggcgtgga tgatgcggtc agtaacggga tgcagctgca
     3121 gctctttcag gatggctttg tgtgcttttc cgccgatctc gagatcgatg gtgtttactt
     3181 ccggcgtgta aacgagcgtt ttgaaagcgc tcagttcaac agagaaagcg acctgttcgg
     3241 gtccgccgta aagcacacaa ggcaccattc cgttcctgcg cagctccttg gcatcttttt
     3301 tccctacgtt cgcacggggc gaaccgctca tagctactga tttcatggta cttgtttttg
     3361 gtttttattg atggatgaaa aaaattacat gatgaaatgc gagctgatcg actcgtagtt
     3421 cacgacgcgg tggaaaacgt cggcgaacat ttccgccacc gaaagcactt tgatcttcgg
     3481 cgattcacgt ttcagcggaa tggtgtcggt tacgataagc tcggtgatct gcgacttctc
     3541 gatgttctcg tacgctttcc cggaaagaac gggatgggtg atgaaggcac ggacgctcgt
     3601 tgccccgcgt tccatcatca tatccgcggc tttgcaaagc gttccgcccg tatccacgat
     3661 gtcgtcgaga agaacgatgt cttttccttc gatgtcgccg atcgcggtga tgtgatccac
     3721 cacgttggct ttcgaacgct gcttgtagca gatcacgatg tcgcacttga gatgcttcgc
     3781 atacgcattc gcgcgcttgg atccgcccat gtcgggagcg gccatcgtga ggttcttcag
     3841 cttcagcttt tcgatgtacg gaatgaaaat ggtggacgcg aaaaggtgat cgaccggcac
     3901 gttgaaaaat ccctggatct ggtcggcgtg aaggtccatc gtcatcacgc ggttgacgcc
     3961 ggccgtggcc agcatatccg ccacgagctt ggccccgatg gccacgcgtg gctggtcctt
     4021 ccggtcctgg cgcgcaaacc cgaaataggg aatgacggcg atgatctctt tcgcggaagc
     4081 gcgtttggcg gcatccacca tcagcagcag ctcgaaaaga ttatcggtgg gaggaaaggt
     4141 cgactggacg aaaaacacgg tggcaccgcg aacggtttct tcaagggaag tctggaactc
     4201 gccgtcgctg aatttatgca gcgtcacctg gcccaggttc tcgccgtatg cggcagcgat
     4261 cttgtccgca atgtaatgcg aaacagatcc ggagaatatt ttgacgtgct cagccacttt
     4321 ttcctgtttt tctaaggggt gcaaatgtaa ggaaatcggg aaagaccgcc aatgaaaaac
     4381 ggctaaaaat cccggatctc atgtacgacg cggtaggcaa tgtccgggcg gtcgggcatg
     4441 ggaatccaca atttccctaa aatattggtt tttagtgaaa tagacccaag gcaactatgg
     4501 cctcgcgtat catagagatc gacaaaatac accgtcccgg gacgaagacc gtctacgc
//
LOCUS       DASTSF010000033         3776 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_195984,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000033 DASTSF010000000
VERSION     DASTSF010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3776
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195984"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(262..996)
                     /locus_tag="VFU15_00925"
     CDS             complement(262..996)
                     /locus_tag="VFU15_00925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716360.1"
                     /translation="MRQRKFSAVFGNVIDVLLAVSVWVFSESLGERALGFHPEKYRDE
                     LFAALCMAQLIFGPLLLIYFIGQWKEDGSGEPEFIRELNRNAFLPVHSGISIFANIVG
                     PLCGFVLPVVFMVVYRNRSAVGTEMLMAMLPALAGMFCVTWAATSGAARRVHIALTSS
                     AGRIMLAVAGWLYVEILLVIMLLILKAPADIEDRGGGQGWLLLMAIGLGYAPARLALF
                     YATGKRNLDFNLAVASILFVTWKLLF"
     gene            complement(1004..2095)
                     /locus_tag="VFU15_00930"
     CDS             complement(1004..2095)
                     /locus_tag="VFU15_00930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716361.1"
                     /translation="MEPVPRIFRRRFSLLVLLVFSTVQATASTLTFSITFKITEYNPP
                     GKRNFPLDNCIFSIRKNDLADSSFTCPDSLEKFILPFGNDYVISIGKSGYVTKRFGVS
                     TKNIPEKREKEPFNEMLVETSLFRLIPGSDYSCLDNPVAKIYFNAAPDVDDFDYDRAY
                     TDSVSRCIQRLMTGQAEAEKDTSQGTLRKKDNGYSLAVYRGDSAVKAENWAAAKMFYQ
                     QAHEIKPGEIYPVDQLAACDLHIKREAMKQDYEYDIAAGDSCFLEKKWNDAQKFYLGA
                     SGLMPGETYPAKLISFCRTETSLVNKSDTALSANEYSFFIRIADEDLSFGRTKEAARR
                     YRMALAAKPGDLYAKNQLAKISGETKREN"
     gene            2130..3269
                     /locus_tag="VFU15_00935"
     CDS             2130..3269
                     /locus_tag="VFU15_00935"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018479727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU4716362.1"
                     /translation="MQTQLSFWEKETYFTGIDVVIAGSGIVGLNAALHLKKKLPRLNI
                     LVAEQGALPSGATSKNAGFACFGSVSELADDLKDRSEDAVFSLVEKRFLGLKRLRENL
                     GDAAIDFHEWGGYEVFDDEKNYEECAEKISGFNARLKDITGRAQTYSIADEKIPAFGF
                     SGVKHMILNSAEGQINTGMALEAMLTKARNAGIRVLNGLFIEDIHEEGNRISIRCGNG
                     YSFTAKKLLICTNGFAKQFLPAEDVQPARAQVLVTSPIENLKFRGTFHYDKGYYYFRN
                     IGERVLFGGGRNLDFKGETTTEHSLTAGIQQKLDELLRTMILPGVNYSVEQRWAGTMG
                     VGKTKSPVVKKVSAAIFCAVRMGGMGVALGSLTGEEAAELVAREM"
     gene            complement(3276..>3776)
                     /locus_tag="VFU15_00940"
     CDS             complement(3276..>3776)
                     /locus_tag="VFU15_00940"
                     /inference="COORDINATES: protein motif:HMM:TIGR00697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HEU4716363.1"
                     /translation="RLSVLTCFMIAFAFLLVFSAMQFKTILKSPSDEEFNNVFGQSLW
                     VIVGSIIAFLISQLVDNSVFWFFRKRTGEKMIWLRSTGSTVISQLIDSFIVLYIGFKL
                     PGKLPADHGFWEIGLTNYVIKLLIAVALTPGIYLFHWFIVRYLGKEAAHVQEEQVVEE
                     SENQNI"
BASE COUNT          961 a          992 c         1002 g          821 t
ORIGIN      
        1 tgcgggaggc ggagcctggg caaaaaccga aacggccggc gacaggacca gcaacagaag
       61 gaagaaaata cgagctaatg cgccgatacg ggcgaaaccc ttcataatac aggaaagtgg
      121 tgctttctga agcatcaaat atcgttcaaa attccttatt tccctcgcaa agacaggtta
      181 gggaaacata aaaagataag cattttctgt gccatgcgtg aacagcaata gtctgcccgc
      241 tccccggctc cggcccaaag ctcagaaaag cagtttccag gtgacgaaaa gaatggaggc
      301 aacagcgagg ttgaaatcga ggttcctttt tcccgttgca tagaacaggg ccagccgtgc
      361 cggcgcatag cccagcccta tcgccatcag cagcagccag ccctggccgc cgccgcggtc
      421 ctcaatatcc gcgggtgctt tcaggataag cagcatgatg accagcaata tttcgacgta
      481 aagccagccg gccactgcga gcattatgcg accggccgag cttgtgagcg caatgtgtac
      541 gcgccgcgct gcaccggacg tggccgccca ggtaacgcag aacattcccg ccagcgccgg
      601 aagcatggcc atgagcattt ccgtgcccac cgccgagcgg ttacggtaaa cgaccatgaa
      661 aacgaccggg agcacaaaac cgcagagcgg cccgacgatg tttgcgaaaa tgctgatacc
      721 ggaatgaacc ggaagaaatg cattcctgtt cagctcgcgg ataaactccg gctctccgct
      781 gccatcttct ttccattggc cgataaaata aatgagcagc agcgggccga agatcagctg
      841 tgccatacag agcgccgcga aaagctcatc gcggtatttt tcgggatgga acccgagcgc
      901 acgttcgccc agcgattcgg aaaataccca aaccgaaacg gcgagaagga cgtcgatcac
      961 gttaccgaaa acggcgctga acttccgctg tctcatttct gtttcagttc tctctcttcg
     1021 tttccccgct gatcttcgcg agctggtttt tcgcgtagag atcgccgggc tttgccgcaa
     1081 gcgccatccg gtaacgccgg gccgcttctt tcgtccgccc gaaactgaga tcttcatcgg
     1141 caatgcggat gaaaaagctg tattcgtttg cggaaagagc tgtatcgctt ttgttcacca
     1201 ggctggtttc agtcctgcaa aaagaaatga gctttgccgg gtaagtctcg ccgggcataa
     1261 gccccgatgc gccgaggtaa aatttctgcg catcgttcca ttttttttcc aggaaacagg
     1321 aatcgccggc agcaatatcg tattcgtaat cctgtttcat cgcttcgcgt ttgatgtgca
     1381 gatcgcaggc ggcaagctgg tccaccgggt aaatttcacc gggcttgatt tcatgcgcct
     1441 gctggtaaaa catcttcgca gcagcccagt tttccgcttt aactgctgaa tccccgcggt
     1501 aaacggcaag cgaataaccg ttatcttttt tccgcaacgt cccttgcgaa gtatcctttt
     1561 ccgcttctgc ctgtcctgtc ataagccgtt gaatgcaacg cgagactgaa tccgtataag
     1621 ccctgtcgta atcgaaatca tcaacgtcag gcgcagcgtt gaagtaaatt ttcgcaaccg
     1681 ggttatcgag gcaggaataa tcactcccgg gaatcagacg gaaaagactt gtctccacca
     1741 gcatttcatt gaacggttcc ttttctcttt tttccggaat attctttgtt gataccccga
     1801 agcgcttcgt gacgtaaccg gatttcccaa tggaaatgac ataatcattg ccgaatggca
     1861 ggatgaattt ttccaatgaa tccgggcagg taaaagaaga atccgcaaga tcgttcttcc
     1921 gtatgctgaa tatgcaattg tccagcggaa aattcctttt tccaggcggg ttgtattctg
     1981 ttattttgaa ggtgatggaa aaagtcagcg ttgatgccgt cgcctgaaca gtggaaaaca
     2041 ccagcagcac caacagcgaa aatctgcgcc tgaaaatccg gggtacaggt tccatgacta
     2101 catttatcac ctaaaattaa tcgattacca tgcagactca gctcagtttc tgggaaaaag
     2161 aaacctactt cacgggcatc gacgtcgtta tcgcgggcag tggcatcgtg ggactgaacg
     2221 cagcgctgca tctcaaaaaa aagcttcccc ggctgaacat tctcgttgca gagcagggcg
     2281 cattaccgag cggggcgaca tcgaaaaatg ccgggtttgc gtgcttcgga agcgtttcgg
     2341 aactggcgga tgaccttaag gacagaagcg aagacgctgt tttttcactg gtggaaaaac
     2401 gttttctcgg gctgaagcgc ctgcgggaaa atctcggtga tgcggcgatc gatttccatg
     2461 aatggggcgg ctatgaagtt ttcgatgatg aaaaaaatta cgaagagtgc gcggaaaaga
     2521 taagcggctt taatgcgcgg ctgaaagata tcaccgggcg ggcgcaaacg tattccatcg
     2581 ccgatgaaaa aatccccgca ttcggttttt ccggcgtgaa gcacatgatc ctgaattccg
     2641 ccgaaggcca gatcaacacc gggatggcgc tggaagcgat gctcacgaaa gcgaggaacg
     2701 ccggcatccg cgtcctcaac ggacttttca tcgaagacat tcacgaagaa ggaaaccgca
     2761 tttctatccg ctgcggcaac ggctactcat tcactgcgaa aaaattgctc atctgcacga
     2821 acgggtttgc gaaacaattc cttcctgcgg aagacgtgca gcccgcacgc gcccaggtgc
     2881 tcgtgacctc gcccatcgaa aacctgaagt tccgcggcac gttccattac gacaaaggct
     2941 attattattt ccgcaacatc ggcgagcgcg tgcttttcgg cggaggcaga aatctcgact
     3001 ttaaaggcga aacgacaacg gagcattcgc tgacggccgg catccagcaa aaactcgatg
     3061 agctgctgcg cacgatgatc ctgccgggcg ttaactattc cgtcgaacag cgatgggccg
     3121 gaaccatggg cgtaggaaaa acgaaatcgc cggtggtgaa aaaagtatcc gcagctattt
     3181 tctgcgcagt gcgcatgggt ggaatgggtg tcgcgctcgg aagtcttacg ggcgaggaag
     3241 ctgcggaact tgtggcccgc gagatgtgaa gcaggtcaga tattctgatt ctcggattct
     3301 tccaccacct gttcttcctg cacatgcgcc gcttcctttc ccaggtagcg gacgatgaac
     3361 cagtggaaaa gataaatgcc cggtgtgagc gcgacagcga tgagcaattt gatgacataa
     3421 ttcgtcagcc cgatctccca gaaaccgtga tccgcgggaa gctttcccgg gagcttgaaa
     3481 ccgatgtaca gcacgatgaa ggaatcgatc agctgtgaaa tgacggtaga gccggtagag
     3541 cgcagccaga tcattttttc gcccgtgcgt ttgcggaaaa accagaacac gctgttgtcg
     3601 acgagctggc tgatgaggaa cgcgatgatg cttcccacga tcacccacag cgactggccg
     3661 aagacgttat tgaattcctc gtcgctcggt gatttgagaa tggttttgaa ctgcatcgcg
     3721 gaaaacacga gcaggaacgc gaacgctatc atgaagcacg tgagcaccga caggcg
//
LOCUS       DASTSF010000034         3265 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_195921,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000034 DASTSF010000000
VERSION     DASTSF010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3265
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195921"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..301)
                     /locus_tag="VFU15_00945"
     CDS             complement(<1..301)
                     /locus_tag="VFU15_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015808136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HEU4716364.1"
                     /translation="MAFRKVKFIEVKSEIGAGTRGASLGPDAIKIAALDYGSNMFRQI
                     ESTEIKNENQLLFESPGSIYAKRIKGLITLYERISAEIQKTVKNNGFPIVLAGDHS"
     gene            407..1804
                     /locus_tag="VFU15_00950"
     CDS             407..1804
                     /locus_tag="VFU15_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009596543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HEU4716365.1"
                     /translation="MENTYQNLIEQTFNFPQEGFNVIDNELYFNDIPLMDIIKKYGTP
                     LKITYLPKISQQIQKAKRLFHVAMAKVDYKGSYTYCYCTKSSHFSFVLEEVLKNDVHL
                     ETSSAYDLYIIKELYKKGVISKDRYIVCNGFKRKTYQQLVTEIANEGFNVIPVLDNKT
                     EIDYYTKYCKVAKMKLGIRIASEEEPNFQFYTSRLGIRHSDIIQYYKDKIQSNPKFEL
                     KMLHFFINTGIKDTIYYWTELAKCMKVYLELKQICPTLDSLNIGGGMPIKTSLGFEYD
                     YEYMIEEIISQIKNFCGNNDIPEPNIFTEFGSFTVSESGAALYSVVDMKLQNDRELWY
                     MIDSSFITTLPDTWGINQRFILLAVNHWDKEYQPVNLGGLTCDSMDYYNTEAHANNVY
                     LPKVTPNTKPPLYIGFFHTGAYQESLGGYGGIQHCLIPAPKHVIIDMDEEGKINTRLF
                     AKEQSYKSMLKTLGY"
     gene            1981..2658
                     /locus_tag="VFU15_00955"
     CDS             1981..2658
                     /locus_tag="VFU15_00955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716366.1"
                     /translation="MRNRIFGAAAIFFLWSLFASCSNFFKKDIKEGVIEFKAEAVGPK
                     EHASMTVPDKMTVKFKDNYSAAELEAGFGFARMKFISDPVKKQFYSQVVFVDKKQSVM
                     DMDEIRKTNYYFPDYDVIYGNGTREIAGYKCKNATLKFKDGSDPIEVWYTKDIAIKDP
                     NWSNAYYKVDGVLMDYRLKKFGLELHFTATSVTDAKIDQSYFCVPPDFGKVRNAELEA
                     MFKGFYD"
     gene            2756..>3265
                     /locus_tag="VFU15_00960"
     CDS             2756..>3265
                     /locus_tag="VFU15_00960"
                     /inference="COORDINATES: protein motif:HMM:NF024882.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HEU4716367.1"
                     /translation="MAEKEPEKKTNRLRNELKPSAKKDADKKDADSADESGVKDSPEK
                     EKKAREKKPQKERPKTGSVNPVRALYTRITSNEKLRRISGFFFLLIAAPYLAVAFTSY
                     LFTWQADQDKVMGPFSELMFGKEDVSNILGKFGALISHIFIHGWFGVTSFFFPFIFFL
                     AGLRLLAGIK"
BASE COUNT          895 a          868 c          765 g          737 t
ORIGIN      
        1 tactgtgatc tccagcgagg acgatcggga acccgttgtt tttcactgtt ttctggatct
       61 cggccgaaat acgctcatat aaggttatca gtcccttgat ccgcttcgca tagatgctgc
      121 ccggcgactc gaaaaggagc tggttttcgt tctttatttc cgtcgattcg atctggcgga
      181 acatattgga gccataatcg agcgccgcga tcttgatcgc atccggaccc aggcttgcac
      241 cacgggtgcc ggcaccgatc tccgatttca cctcaatgaa tttgactttt ctgaaggcca
      301 ttgtaataaa tttatcgtgt taacagattt aacatccggg tgacttacat ttgcattccc
      361 gaaccataag taactcactt actttgaccc aatgcaacaa ggcaaaatgg aaaatacgta
      421 ccagaacctg atcgagcaga cgttcaattt tccccaggaa ggatttaacg tcatagataa
      481 tgagctgtat ttcaatgata tcccgctaat ggacatcatc aagaaatatg gaactccgct
      541 caagatcacg tacctcccca aaatcagcca gcaaatacag aaggccaagc gccttttcca
      601 cgttgccatg gcaaaagtcg attacaaagg tagttatacc tactgttact gtacgaaaag
      661 ttcccacttt tcatttgtgc tggaggaagt gctcaagaac gatgtgcacc tggaaacctc
      721 atccgcgtat gatctttaca tcatcaagga gctgtacaag aaaggcgtga tcagcaagga
      781 ccggtacatt gtatgcaatg ggttcaagcg caaaacctac cagcagctgg tgacggaaat
      841 agcgaacgaa ggttttaacg tcatacctgt cctggacaat aaaacagaga tcgattacta
      901 caccaagtac tgcaaggtgg ccaaaatgaa actcgggatc cggatcgctt cggaagaaga
      961 gccgaatttc cagttctaca cgtcccgcct cggcatccgc cacagcgaca ttatccagta
     1021 ttacaaggac aagatccagt cgaacccgaa gttcgagctg aagatgcttc actttttcat
     1081 caacacgggc atcaaggata cgatctatta ctggacagag ctggccaaat gcatgaaagt
     1141 gtacctcgag ctcaagcaga tctgcccgac gctcgattcg ctgaatatcg ggggcggaat
     1201 gccgatcaag acctcgctcg gattcgaata cgattacgag tacatgatcg aggagatcat
     1261 ttcgcagatc aagaatttct gcggcaacaa cgacattccc gaaccgaata ttttcaccga
     1321 gttcggctcg ttcacggtga gcgaaagcgg cgccgcgctt tactccgtgg tggacatgaa
     1381 gctgcagaac gaccgcgagc tctggtacat gatcgacagc tcctttatca ccacgctgcc
     1441 cgacacctgg ggcatcaacc agcgtttcat cctgcttgcc gtcaatcact gggataagga
     1501 ataccagccc gtcaacctcg gcggcctcac ctgcgacagc atggattatt acaacaccga
     1561 ggcgcacgcg aacaacgttt accttccgaa agtgacgccg aatacgaagc cgccgcttta
     1621 tatcggcttt ttccataccg gcgcttacca ggaatcgctc ggcggatatg gcggaataca
     1681 gcattgcctc atccccgcgc ccaagcacgt gatcatcgac atggacgaag aaggcaagat
     1741 caacacgcgc ctttttgcca aagaacagag ctacaaatcc atgctcaaga cgctgggcta
     1801 ctgagaccgg ttaatggagg caatacggtg tttcgtctgt catattccgg catttacccc
     1861 ggcaaacgca aaaatccggc tcttgaaatt caagaattta tagctacctt gccccgacct
     1921 aatcgcggac ctggtctgcg atttttaaaa ttcctccgga ccactcgcta catctggaat
     1981 atgagaaaca gaattttcgg tgccgccgcg atcttctttc tgtggtctct tttcgcttcc
     2041 tgttccaatt tcttcaaaaa agacatcaaa gaaggcgtta tcgagttcaa ggctgaagcc
     2101 gtgggcccca aagaacacgc ttccatgacg gtgccggata agatgaccgt gaagttcaag
     2161 gacaattatt ccgccgccga gctcgaagca ggattcggtt ttgcccgtat gaaattcatc
     2221 tccgacccgg tcaaaaaaca attctacagc caggtggtat tcgttgacaa aaaacagagc
     2281 gtgatggata tggacgagat ccggaaaacg aattattatt tcccggatta cgacgtgatc
     2341 tacggcaacg gaacgcgcga gatcgccggc tataaatgca aaaacgccac gctgaaattc
     2401 aaggacggat ccgacccgat cgaagtgtgg tacacgaaag acatcgcgat caaagatccc
     2461 aactggtcga atgcctatta caaagtcgac ggcgtgctga tggattaccg cctgaaaaaa
     2521 ttcggccttg agcttcactt caccgccacg agcgtcaccg acgcgaagat cgaccagagc
     2581 tacttctgcg tcccgcccga tttcggcaaa gtgcgcaatg cggagctgga agccatgttc
     2641 aaaggcttct acgactgatc ccgtttaaac agatgaatgt tcaaaacccg ctcttttatg
     2701 aaaaatgagc cggtctgccg ttggctactt ttgttattta caaccgccgc catccatggc
     2761 agaaaaagag cccgagaaaa aaacgaaccg cctccgcaac gagctgaagc cttccgcgaa
     2821 aaaagatgcg gacaagaagg acgcggacag tgcggatgaa agcggcgtaa aggattcccc
     2881 ggaaaaagaa aaaaaagcgc gggagaaaaa gccgcagaag gaacgcccga aaacaggcag
     2941 cgttaatcct gtccgcgccc tgtacacgcg catcacctcg aacgaaaagc tgcgccgcat
     3001 cagcggattt ttctttctcc tcatcgcggc gccgtatctc gctgttgcat tcacctcgta
     3061 cctcttcacc tggcaagctg accaggacaa ggtgatggga ccattctccg aactgatgtt
     3121 cgggaaagaa gatgtatcca acatactcgg aaaattcggc gcgctgatct cgcatatttt
     3181 catccacggc tggttcggcg tgacatcgtt ctttttcccg ttcatttttt tcctggcggg
     3241 actgcgcctg ctggccggca taaag
//
LOCUS       DASTSF010000035         4661 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_660681,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000035 DASTSF010000000
VERSION     DASTSF010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4661
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_660681"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            67..483
                     /locus_tag="VFU15_00965"
     CDS             67..483
                     /locus_tag="VFU15_00965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716368.1"
                     /translation="MRKKRFHFMHEEKIGLLFPCEKSWKEMTPAGSGKHCSACNQVVK
                     DFTRMSLPEIQRFFSAHEGKKICGRYRAEHVSGPNVKPPSAFRKMLNTVFVFLGISLL
                     IQSCIGERTPRNPAFYRQDSTVKKNLKAAPQQVKKP"
     gene            complement(496..1449)
                     /locus_tag="VFU15_00970"
     CDS             complement(496..1449)
                     /locus_tag="VFU15_00970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716369.1"
                     /translation="MGRHCHVPFPHLDREKTETYFLNYKFSPAIKERKSVISGEEELH
                     RKLLDNIKNRHAHRRYRGYFDMRLLFLRPGFIPPETGNASPSVPIGTQEMSLIARYDG
                     IHQDIHELNGIVRTQNEGNVIFEFDGKTCWSRDVERVKKLLMEEDKELFEKLSEADAQ
                     LYSWHYSTGNAEEKKVLLQLAPFYSHCEKLRQLTAGQAQAFNYYANLMTTYKGSINDI
                     LVQVKQQFEVYEQDRDKISTALGACAVDSLDDESRQAYERLCHSLRAVPGVNTKKYSL
                     ALFKEMKSAVEKVAAESHRSAWLLNGKILRLFDGISGRMRS"
     gene            complement(1359..2573)
                     /locus_tag="VFU15_00975"
     CDS             complement(1359..2573)
                     /locus_tag="VFU15_00975"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEU4716370.1"
                     /translation="MITNPGIERSIEEIRSHIRRKTFGFMLSFALFWLMYLVILAATA
                     AGVLVALYNFPRLLMAGLGGMIIFGALLFISLSAFLIVVRFLFARQNETEEGLIEIPA
                     IEKDPFIAMINEICDEVKAVRPSKILLSHMPNASVSGYGALQGIFRPNEKKLTIGYPL
                     ILFLNRSEFRAVLAHEFAHFSKDSLVPGQFLSRLHKGVFTMLYKKGSIDEWIRDMAHS
                     RGIPGLVGRLAATLSNFIRKLFAQSLENLHLRFMAISRLQEFSADKIAACVAGHKAIW
                     GSLLKTGFLVETIGQLDGQVVENLDTENKLPQHYWTIFSGLISFRLEHEGIALPLQET
                     TAPRSEREKNIIRRLKTGDAWHAHPSDDDRKKNVEGIGAELWEDTAMFHSRTSTGKRP
                     KRISSTTNSVPP"
     gene            complement(2958..4424)
                     /locus_tag="VFU15_00980"
     CDS             complement(2958..4424)
                     /locus_tag="VFU15_00980"
                     /inference="COORDINATES: protein motif:HMM:NF021597.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2330 domain-containing protein"
                     /protein_id="HEU4716371.1"
                     /translation="MKKIISTVSAMAIAYSSFGFCGFYVAKADTKLFNKTSQVIIARN
                     GDHSTITMSSDFEGDVKDFAMVVPVPVVLEQKDIKVVDRILFDKLDAYSGPRLVEYYD
                     QDPCYAPRYYDYMVPAMARSNAGAAEDDIVMDSAAKKDYGVTVEAKYTVGEYDILILS
                     AKESGGLYDWLSDNGYKVPDGAKEVLDPYITGGMKFFVAKVNLDNYQNNGAVPLRPLQ
                     ISFNSPKFMLPIRLGMANADGPQDLIVYAFSKTGRIETTNYRTVPVPTGQNVPLFVAD
                     EFGSFYKNLYNKAWKRENQNTVFLEYAWDVSPQVTSYCDPCTGPPPMLDDFQQAGVDW
                     VENYHYGYQGEVHFTRLHITYARNTFPQDLMFEETPNKENFQARYVITHPASSDFSCD
                     AGQDYLKELSKRRWNELWTYQWLTGNAVTDHYDYPAEYNKFLKKENKKTGDFIPPFIP
                     PQGNNGPDGGMMSIVLSFLTLAAVMFTLSRRRQPAAVA"
     gene            complement(4461..>4661)
                     /locus_tag="VFU15_00985"
     CDS             complement(4461..>4661)
                     /locus_tag="VFU15_00985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+-transporting NADH:ubiquinone oxidoreductase,
                     subunit NqrB"
                     /protein_id="HEU4716372.1"
                     /translation="FMITDPMATPFSRRARIIWSTLVAIVAWALSVQYLQPTTPLWAL
                     FFLSPLVPFFDRFMPGKKFRWQ"
BASE COUNT         1045 a         1192 c         1282 g         1142 t
ORIGIN      
        1 ccctttctcg ccgaacctga tttccgccgg acgggtgatg cggtgatcag cgtgtgagca
       61 atttcaatgc gtaagaaacg ttttcatttc atgcacgaag aaaagatcgg tcttcttttt
      121 ccctgtgaaa aaagctggaa ggaaatgacg cctgccggga gcgggaaaca ttgttctgcc
      181 tgcaaccagg tggtgaagga tttcacgcgc atgtcgctgc cggagataca gcggttcttt
      241 tcggcgcacg aaggaaaaaa gatatgcgga cgttaccgcg cagaacatgt ttccggtcct
      301 aatgtgaaac ctccgtccgc tttccggaaa atgctcaaca cggttttcgt tttcctcggg
      361 ataagcctgc tgatccagtc gtgcattgga gaacgcacgc cacgcaaccc ggcattttac
      421 cggcaggatt caacggtgaa gaaaaacctg aaagctgcgc cgcagcaggt aaaaaagccg
      481 taaaaccgtt tcacgttacg atcgcatgcg cccgctgatc ccatcgaaca gccggagtat
      541 tttaccatta agcagccacg ctgagcggtg cgattctgcc gccacttttt ccactgcgga
      601 tttcatttct ttgaacagcg cgagcgaata ttttttcgtg ttgacgcccg gcactgcgcg
      661 cagtgaatgg caaagccgtt cgtacgcctg ccgcgattca tcgtcgaggg aatccaccgc
      721 acaggcgccg agagcagttg aaattttatc acggtcctgc tcataaacct cgaactgctg
      781 tttcacctgc acaagaatat cgttgatgct gcccttgtac gtcgtcatca ggttcgcata
      841 ataattgaaa gcctgtgcct gcccggcggt caactggcgg agcttttcgc aatgagaata
      901 aaacggggcg agctggagga gcaccttttt ttcttccgca tttccggttg aataatgcca
      961 gctgtaaagt tgcgcgtctg cctccgaaag cttttcgaat aactccttgt cctcttccat
     1021 cagcagcttt ttcacgcgtt ccacatcgcg cgaccagcag gttttcccgt caaactcgaa
     1081 gatcacgttc ccttcgtttt gcgttcggac gatcccgttc agctcgtgta tatcctggtg
     1141 gatcccgtca taacgtgcga tgagcgacat ctcctgcgta ccgatgggaa ccgacggcga
     1201 agcattgccg gtttccggcg gaatgaaacc gggacgaagg aaaagaaggc gcatgtcgaa
     1261 atagcccctg tacctccggt gcgcatgccg gtttttgata ttgtccagca gtttccggtg
     1321 gagctcttct tcgcccgaga tgacggactt tctttccttt atggcgggac tgaatttgta
     1381 gttgaggaaa tacgtttcgg tcttttcccg gtcgaggtgc gggaatggaa catggcagtg
     1441 tcttcccaca attcggcacc gatgccttcc acgttttttt tccggtcatc atccgaagga
     1501 tgcgcatgcc aggcatcacc ggtttttaaa cgccgtatga tattcttctc gcgttcagat
     1561 cggggagcag tggtttcctg cagcggaaga gctattcctt cgtgttccag ccggaaactg
     1621 atcagcccgg aaaatatcgt ccagtaatgc tgcggcagct tgttttccgt gtcgagattt
     1681 tccacgacct ggccatcgag ctgcccgatg gtttcgacaa ggaatcctgt tttcagcagc
     1741 gatccccaga tcgctttatg gccggccacg caggctgcga ttttgtcggc actgaattcc
     1801 tgcaggcgcg agatcgccat gaaacgcagg tgcaggttct cgagcgattg cgcgaatagt
     1861 ttgcggatga aattgctcag cgtcgctgcg agtcgcccga cgagaccggg aatgccccgt
     1921 gaatgcgcca tgtcccggat ccattcatcg atgctgcctt ttttgtagag catcgtgaaa
     1981 acacctttgt gcaggcgact gaggaactgg cccgggacaa gcgagtcttt ggaaaaatgc
     2041 gcgaattcat gcgcgagcac ggcgcggaat tcactgcggt tgaggaacag gatgagcggg
     2101 tagccgatcg tcagcttttt ttcattggga cggaagatgc cctgcagcgc cccgtaaccg
     2161 ctcacggatg cattgggcat gtgagacagg aggatcttcg atgggcggac cgctttcact
     2221 tcatcgcata tttcgttgat catcgcaatg aacgggtcct tttcgatggc aggaatttca
     2281 atgagccctt cctccgtttc attctgccgg gcgaaaagaa aacggaccac gataaggaac
     2341 gcagacagcg agatgaacag gagcgccccg aaaatgatca tcccgcccag gcctgccata
     2401 agcaggcggg gaaaattgta cagggcaaca agaacgcctg ccgctgtggc ggcaaggatg
     2461 accaggtaca tcagccagaa cagcgcgaac gaaagcatga acccgaaggt cttcctgcgg
     2521 atgtgcgatc gtatttcctc aatggagcgt tcaatcccgg ggttggtgat catacgttac
     2581 ggaaagatca gaaatttccg gcgcagccct gcattttacc gcgttttttc aggacccggt
     2641 gcccggttcc ttttgttttc gaaccaattt taggcaactg gttgcaggat gcggttcctt
     2701 tcatccgcag cttttttcga aataattctt gtgcggaaaa aaattctgcc ggtattttat
     2761 tcaggaaaat tcaatcgcat gaaaaaaata attccggctg tcatccgaac ggcaaacggc
     2821 tatgtcatga tctgcaccgg cgacccgtat gtcgcttccg ttccggaaaa ctgccggaaa
     2881 ccggtttaaa cgcaaagatc cacggagaga atctctgtgg atctttgttc aggcgcgcgg
     2941 ggtttgcgcg gatttcttta cgccaccgca gccggctgcc gtcgtctcga cagtgtgaac
     3001 atcactgcgg cgagcgtgag gaaggaaagc acgatgctca tcattccgcc atccggtccg
     3061 ttgtttccct gcgggggaat gaacggggga atgaagtcgc cggttttctt gttctctttt
     3121 ttcaggaatt tgttgtactc ggcggggtaa tcgtagtgat cggttaccgc gttcccggtc
     3181 agccactggt aggtccacag ttcattccag cggcgcttgc tcagctcttt caggtagtcc
     3241 tgcccggcgt cgcatgagaa atcgctgctc gcaggatgcg tgatgacgta acgcgcctgg
     3301 aaattttcct tgttgggcgt ttcctcgaac ataaggtcct gcgggaacgt gttgcgtgca
     3361 tacgtgatgt gcagtcgtgt gaagtgaact tctccctggt aaccgtaatg gtaattctcc
     3421 acccagtcga caccggcctg ctggaaatca tcgagcatgg gcggtggccc ggtgcacgga
     3481 tcgcagtacg atgtcacctg cggacttaca tcccacgcgt attcgaggaa aacggtgttc
     3541 tggttctcgc gtttccacgc tttgttgtac aggtttttgt agaatgaacc gaactcatcc
     3601 gccacgaaaa gcggaacgtt ttgtcctgtg ggaacgggaa cggtgcggta attggtcgtt
     3661 tcgatgcggc ctgttttcga aaacgcataa acgataagat cctgcgggcc atcggcgttc
     3721 gccatgccga gacggatggg cagcatgaac ttcggtgaat tgaacgagat ctgcagcggg
     3781 cggagcggaa cggcgccgtt attctggtaa ttgtcgaggt tgacttttgc gacaaagaat
     3841 ttcatgccgc ccgtgatgta cgggtcgaga acttctttcg caccgtccgg aactttgtag
     3901 ccgttgtcgg aaagccagtc gtaaagtccg cccgattctt tcgcggaaag aataagaatg
     3961 tcgtattcgc cgacagtgta tttcgcttca acggttacgc cgtaatcttt tttcgccgct
     4021 gaatccatga cgatgtcatc ttctgcagct cctgcgttcg agcgcgccat ggcaggaacc
     4081 atgtaatcat aatagcgcgg cgcgtaacag ggatcctggt cgtaatattc caccaggcgc
     4141 ggaccggaat acgcatcgag cttgtcgaag agaatacggt cgacgacctt gatgtctttc
     4201 tgctcgagca caacgggaac gggaacgacc atggcgaaat cttttacatc gccttcgaaa
     4261 tcgctcgaca tcgtgatggt ggaatgatcg ccgttgcgtg cgatgatcac ctgcgacgtt
     4321 ttgttgaaga gctttgtgtc ggccttcgcg acatagaaac cgcagaagcc gaaggagctg
     4381 taagcgatcg ccatcgcgga aacggttgag attatttttt tcatggactg aatgatttta
     4441 cgtgaatgaa aaacaggatt ttactgccac cggaattttt tacccggcat gaatctgtcg
     4501 aagaaaggaa caagcggtga aaggaagaac agcgcccaga gcggggtcgt gggctgcagg
     4561 tattgaacgg acaatgccca cgcgacaatt gcaacaagcg ttgaccagat gatgcgtgcc
     4621 ctgcggctga acggtgtggc catcgggtcg gtgatcatga a
//
LOCUS       DASTSF010000036         4471 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_213281,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000036 DASTSF010000000
VERSION     DASTSF010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4471
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213281"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..463)
                     /locus_tag="VFU15_00990"
     CDS             complement(<1..463)
                     /locus_tag="VFU15_00990"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HEU4716373.1"
                     /translation="MKRLLFVGVLAVLLFGSCQQENGQLMGVQNRPAWYPFDPYGMLY
                     CPMGSYNMGPSDEDIPYAHTTKSKTVSVQAFYMDQTEISNNEYRQFVYYVRDSLARRL
                     LATNGFEDDYLIPQEDWTGYEDNGPRYVDDDFDKLGADYQPLNWQEKIRWDA"
     gene            complement(665..1750)
                     /locus_tag="VFU15_00995"
     CDS             complement(665..1750)
                     /locus_tag="VFU15_00995"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HEU4716374.1"
                     /translation="MNIYLSDLRADCMLKNIWQNTSRDMRKTLTTVAFVLGSIAAFSQ
                     QDPQFSQYMYNKLFMNPGYAGIRQALCATAIYRDQWQGFDGAPKTGVFSADYFSPAIH
                     GGVGLNIMTDKLGFESTMAFRADYSFHVLNLAGGVLGIGVELGAVSKRIGPTGSDSWI
                     ATTNWQSDQSIPPQLKKTVFDAGAGLWYQRQNIWFGISSTHLSAAQVDDGTASITPTS
                     PPGPPVVHNLKYQIAHHYFVTGGINLFQGNTWEIRPSFLVKSDATITSIDISCLAMFN
                     QRFWFGANYRVKDAICPMVGFQIPNKLMTPDGRPDGGLKVGFAYDYTTSDLNNYNNGT
                     FEVFLNYCVPIVVTPKRQGHGDVRIFD"
     gene            complement(1864..2847)
                     /locus_tag="VFU15_01000"
     CDS             complement(1864..2847)
                     /locus_tag="VFU15_01000"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HEU4716375.1"
                     /translation="MKKIITISVFFLACFRLAAQQDPQFSQFMFNKLFMNPAYAGMRE
                     AFCMSAIGRQQWAGFDGSPRSGIFSADMKLPGYAGGIGINVLSDRLGFENNQRYNIDY
                     SYHRELGDGVIGIGVEFGAYSKHIGPTGNDQWQSTTAWTSDPSIPSSLNKTVPDFGFG
                     LWYQRQDLWFGISSSHLNAMMINGGQETILGHTHDQRFQMARHYFITGGCNISTLGNG
                     TWSLRPSFLVKSDATITSFELNCIALWQNKFWGGVSWRYRDAICPMVGFQLLTPHMPD
                     KFSDGGLKIGFAYDYTTSQLHNYNDGTFEIFLNYCIPLDYTAGHGTVRFFE"
     gene            complement(3127..4311)
                     /locus_tag="VFU15_01005"
     CDS             complement(3127..4311)
                     /locus_tag="VFU15_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013664044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamase"
                     /protein_id="HEU4716376.1"
                     /translation="MSNLDFSEFFQPVDAEKIAAGRKFLPTQIGNLLKINTEANGWPE
                     LGEVQVALLGVNEDRRAAHNHGCAIAPDLVRENLYRLFQGDWNLRMADLGDIRAGNTP
                     ADTDFALKTVMGALLKKNILPVIIGGGHDLTYAQYLAYEALEQTVNLVAVDPQFDLGA
                     SGEELGSRSWLSRIILHQPNFLFNFSNIGYQTYFVEQAAIELMGKLNFDIHRLGLSRA
                     KMEDVEPIIRNADIVSIDIGSVRQSEAPGNENASPNGFFGDEICQVARYAGISDKCSS
                     FGIYEINPAFDRKNLTSHLAAQMVWCFLDGYYNRKKDYPFGDISDYTRYRVFLKDHKH
                     EIVFYKSTRSDRWWMEVPYPPNQKLKFERHALVPCSYNDYELACKEEMPDRWWQTFQK
                     LS"
BASE COUNT         1109 a         1070 c         1249 g         1043 t
ORIGIN      
        1 tagcatccca gcggatcttt tcctgccagt tcagcggctg gtaatcagcg ccgagtttat
       61 cgaagtcatc atccacgtag cgcggaccgt tgtcttcgta acccgtccaa tcttcctgcg
      121 ggatcaggta atcatcttca aatccattgg tcgccagaag acgacgcgcc agagagtcac
      181 gaacataata cacgaactgg cggtactcgt tgttcgagat ctccgtctgg tccatgtaaa
      241 acgcctgaac tgacaccgtt ttggactttg tcgtgtgagc atacggaata tcctcatcac
      301 tcgggcccat gttgtagcta cccatcggac agtacaacat accgtacgga tcgaacgggt
      361 accatgcagg gcgattctgc acgcccataa gctgcccgtt ttcctgctgg caggatccga
      421 acaacaatac ggcgagaacg ccaacgaaca acagtcgttt catttttgcc tcctttagta
      481 gtttcccttg tttattctgt gtactctggt gttggtctat ttagaacgct ggcaatgttt
      541 ccggtattgc cccctattgc agaaaatcac cataacctga ggatatgatg atttttaggc
      601 aacagagttt gcttttacgc tagcttttgt aataaagtta caaaaccctg cctaacttcc
      661 tgatttaatc aaagatacga acgtcgccgt gtccctgacg cttgggggta acaacaatcg
      721 gaacgcagta attgaggaat acctcgaatg ttccgttgtt gtaattattg agatcggaag
      781 tcgtgtaatc atacgcaaat cccactttca ggccaccgtc aggacgacca tcgggtgtca
      841 taagcttgtt ggggatctgg aagcctacca tggggcagat cgcgtcttta acgcggtagt
      901 ttgccccgaa ccagaaacgc tggttaaaca tggccaggca gctgatgtcg atagatgtga
      961 tcgtagcgtc agatttgacg aggaatgagg gacgaatttc ccacgtattt ccctggaaga
     1021 ggttgatgcc acccgtcaca aaatagtggt gggcgatctg atatttcaag ttgtgtacaa
     1081 ccggaggtcc cggagggctg gtgggcgtaa tagacgctgt tccgtcatcc acctgtgcgg
     1141 cggacaagtg cgtggaagaa ataccgaacc agatgttctg gcgctggtac caaagtccgg
     1201 cacccgcatc gaacactgtc tttttcagct gtggcggaat ggactggtcc gattgccagt
     1261 tcgtggttgc gatccacgag tcggaacccg taggtccgat acgcttgctg actgcaccaa
     1321 gttcaacgcc gataccgagg actccacctg ccaggttgag cacatggaaa gaataatcag
     1381 cgcggaaagc catcgtgctt tcgaatccca gcttgtccgt cataatgttc aggccgacgc
     1441 caccatgaat agcggggctg aaataatcag ccgaaaaaac gcctgttttg ggtgcgccat
     1501 caaaaccctg ccactggtca cgataaatcg cggtggcgca aagagcctgg cggattccgg
     1561 cataacccgg attcatgaaa agcttattgt acatgtactg cgaaaactga ggatcctgct
     1621 gtgaaaatgc agcgatactt ccgagtacaa atgcaacagt ggtaagggtt tttctcatat
     1681 ctctactggt gttttgccaa atgtttttaa gcatgcagtc ggcacgcagg tcgctaagat
     1741 agatattcat ttttgaataa caaatctttt ttaaccaccc cctgttcaaa atccggacaa
     1801 atgttaaaat ccggttcgcg acagcgccag ttctatgaaa tgagctatgt atttctcctg
     1861 ttttcattca aaaaaacgga cggttccgtg accagccgtg tagtcaagtg gtatacagta
     1921 attcagaaaa atctcgaacg tgccgtcgtt gtaattgtgc agctgcgacg tcgtgtaatc
     1981 gtacgcgaat cctattttga gtccgccgtc gctgaactta tccggcatgt gcggcgtaag
     2041 caactggaaa ccgaccatgg gacaaatggc atcccggtag cgccaggaaa cgcctcccca
     2101 gaatttattc tgccagagcg cgatgcaatt gagctcgaag gaagtgatcg tcgcgtctga
     2161 tttgacgagg aatgatgggc ggagcgacca ggtgccgttg ccgagcgtgc tgatgttgca
     2221 tccgcccgtg ataaaatagt gccgcgccat ctggaaacgc tgatcgtggg tgtgaccgag
     2281 gatcgtttcc tggccgccgt tgatcatcat tgcattgagg tgagaggatg agatcccgaa
     2341 ccagagatcc tggcgctgat accacaggcc gaaaccaaaa tcgggaacgg ttttattgag
     2401 cgaggacgga atggacgggt cggatgtcca ggcagtggtg gattgccact gatcatttcc
     2461 tgtcggaccg atgtgttttg aatacgcgcc gaattccaca ccgatcccga tgacgccatc
     2521 gccgagctcg cggtgataag agtaatcgat gttgtagcgc tggttgtttt caaatccgag
     2581 gcggtcgctt aacacgttta tgccgatacc gccggcgtaa ccgggaagct tcatgtcggc
     2641 ggaaaaaatt ccgctgcgcg gggaaccgtc gaagccggcc cattgctggc gcccgatggc
     2701 tgacatgcag aatgcttcgc gcatgcccgc atacgccgga ttcatgaaca gtttgttgaa
     2761 catgaactgc gagaattgcg gatcctgctg cgcggcgagg cggaaacagg cgagaaaaaa
     2821 aacggatatg gtaattattt tcttcatagg atggatgtgt ttgccgcttg tataacagga
     2881 ggggcgggaa aagatacagg gggattctgg attgttgatt gttgattgtt gattgacgat
     2941 tgttgattgg cgattgacga ttgaaaaccg ggagatcaaa aggtctgcga atgaagagac
     3001 aggaaaatga gaggaaacgg cgaaacatga acagccccgg cagagcggag tcaccatgtg
     3061 cgagcgatga cgggaaagcg ggaacggaaa tgcgcaggtg ttgcgctgca aaacaaaaga
     3121 gagaactcag ctgagcttct ggaaagtctg ccaccagcgg tcgggcattt cttccttaca
     3181 ggcgagctcg taatcattgt acgagcaggg gacgagcgcg tggcgttcaa acttcagctt
     3241 ctgattgggc ggatacggaa cttccatcca ccaccggtcg ctgcgcgtgc ttttgtagaa
     3301 aacgatctcg tgcttgtggt ctttgagaaa aacgcggtag cgcgtgtagt cggaaatatc
     3361 gccgaaggga taatcctttt tgcggttgta atagccgtcg aggaagcacc acaccatctg
     3421 cgcggcgagg tgcgacgtca ggtttttgcg atcgaaagca ggattgatct cgtaaatacc
     3481 gaaggaagaa cacttgtcgc tgatgccggc atagcgtgcg acctggcaga tctcgtcgcc
     3541 gaaaaaacca ttgggagaag cattctcatt gccgggcgct tcgctctggc ggacggaacc
     3601 gatgtcgatg ctgacgatgt cggcgttgcg gatgatgggt tcgacatctt ccattttcgc
     3661 gcgggaaagt ccgaggcggt gaatatcgaa attgagtttt cccataagtt cgatggcagc
     3721 ctgctcgacg aaatacgtct ggtacccgat gttggaaaaa ttgaagagaa aattcggctg
     3781 atgcagaatg atgcgggaga gccaggaacg gctgcccagc tcttcgccgg aagcaccgag
     3841 atcgaactgc ggatcgacag cgaccaggtt caccgtctgc tcgagcgctt cgtaggcgag
     3901 gtattgcgcg taggtaagat catgtccgcc gccgataatg acgggaagga tatttttttt
     3961 cagcaatgcg cccatgaccg ttttgagcgc gaaatccgta tcggcaggcg tgttccctgc
     4021 gcgtatgtca ccgagatcgg ccatgcgcag gttccagtcg ccctggaaaa gccggtaaag
     4081 attttcgcgc acgaggtcgg gcgcgatcgc gcagccgtga ttgtgcgcag cgcggcgatc
     4141 ctcgttgacg ccaaggaggg caacctgcac ctcgccgagc tccggccagc cattcgcttc
     4201 cgtatttatc ttcagcaaat ttccgatctg cgtcgggaga aatttccggc ctgcggctat
     4261 tttttccgca tcaaccggct gaaagaattc ggagaaatcg aggttgctca tgaaggcaaa
     4321 tatacctgcc gaaaatatgc agaagatgag atggaggacg aacagttaac agctcaacgt
     4381 tcaaagttca tgaaagcagg agagttggaa ggtcgggagg tcgggaagtt ggaaaagcgg
     4441 gaaagcgggc cggataaaaa aagttccgct t
//
LOCUS       DASTSF010000037         7470 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1085532,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000037 DASTSF010000000
VERSION     DASTSF010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 7470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7470
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1085532"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..383)
                     /locus_tag="VFU15_01010"
     CDS             complement(<1..383)
                     /locus_tag="VFU15_01010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716377.1"
                     /translation="MKRIILLFGILLSFAAVRAQSATSAAPADAPTPQQKAHTQAMQM
                     QKMLGLSDEQTTSVEALFLTRIQSIDQINNDATKTDQQKKEDIQKVRDQNETDVLAVL
                     TPDQIAKYKQIKADRQNRQQSRAAA"
     gene            511..1068
                     /locus_tag="VFU15_01015"
     CDS             511..1068
                     /locus_tag="VFU15_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020081695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HEU4716378.1"
                     /translation="MEYYRYIMALHVIFVVSWFAALFYMPRLLIYHAEADQKPEPDRG
                     ILSEQLKIMQKRLWGIIAWPALLLTWAMGLWMMYLNPSLLSQPWFILKLLFVSGLTLY
                     HVQTHALYMKHQRDEIPWTSFKLRLWNEVATIFLFAIVFLVIPKQNTGWVWATLGLIG
                     FAAAMFAAVSIYRKAREKKKEEPDA"
     gene            1147..1545
                     /locus_tag="VFU15_01020"
     CDS             1147..1545
                     /locus_tag="VFU15_01020"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU4716379.1"
                     /translation="MDEPTDFNEPLTILIVDDEPDIREFISYFLSHKGYRVFAASNGR
                     EAVDIAKRVIPDLVILDILMPEMNGYETCRVLRSTPGLEMTRILFLSALSESYVGEIS
                     DKLNADGFISKPIRMGMLVKKVEERIRRTG"
     gene            complement(1551..2639)
                     /locus_tag="VFU15_01025"
     CDS             complement(1551..2639)
                     /locus_tag="VFU15_01025"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEU4716380.1"
                     /translation="MDPEINISHYISSLLFRHDCVVVPGLGAFVCTEVPAKIHPARHT
                     FDPPSRRIVFNKHLQQNDGLLAQTIVEESGHSFDEAMKAIAAFAEGIQQSLRAGKKTE
                     LPHIGTFSVDPEGNIGFEQEPDVNYLVEAFGLASFQSMPVIREKEKEPEVRKPVVRDI
                     RTAPEPKPEVPAAGYPLRKLAIAASIILPLLLIGTWFTASYSKDIAGFGFFRHEPSLY
                     KPVQWFNKTKLFSDTTGNSRLVPDQGGIARIESSEKMPPIIVDIHKVLPDSLYVASAV
                     NSNVAPPRSSSADDHYFVIAGCFAVSQNADNFRKALQERGFHAEVVENLRSRLTHVGV
                     GSFRTKEEADRFLSEIRRDIPEAWILRK"
     gene            complement(2775..5186)
                     /locus_tag="VFU15_01030"
     CDS             complement(2775..5186)
                     /locus_tag="VFU15_01030"
                     /inference="COORDINATES: protein motif:HMM:NF040138.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HEU4716381.1"
                     /translation="MNAVRTSAKFYGLVFFCALFMTMAHAQDVPPFAAKVAPADPVVP
                     GTSLPDRSTWVEDESQRTVFSSVYHTADGQYIAHQSSVPINYYDVNGKLQPIDFSPKK
                     ITEGWTAPAQPFPTFLFNDGSAYVSYGKDEGDYFRFGISPVINGVNASPQAIAFDGDT
                     GTMHDVVPGVDRKYAFRLNGIETSYIVRQPQQVNSDNFVVTEGFLLDHGFTLKEDESR
                     GRKENGGWTGDLLVIRESDDAVVGTIKAAMCYDAAGKYTFGTYVIRYDEHTDMELLDI
                     LVPRSWMNDAVRSYPVTIDPLVIGPTALWSGGSMPSCIAPANHTDSILVTIPGQITVT
                     SLAVTSSFYADPFTTAIMSDGAMWFTTPCGSSPQFTIGPPNGNLPGTAYLDTFNLRSP
                     LTCCYAQSCSPQTFYLTMHLSRTGPGTGCNTTYIRYDPVTTSWPFSAYIVGHTVEMNA
                     QGFTVPVTPVCSNVCTFTGNVFIHYGVPPFTITHPWMTGSVTAGSPAGCNYAIVSKQI
                     TLTIPNCPTYCDTITTLTVPPPVVVDACGNIATGYPSRPLHIHEVPVLTVSPPSDTVC
                     PGEPYQFNLNSCLAGSTVSWYNSSGSGNGNVNGSVNYTGTADTSITFNAFASINGCTS
                     DTITFPAVVQPFPSAAFTFSSPAIAGVPVSFTDQSSFYSGAGASWGWDFGDNSVSALQ
                     NPVYTYSSPGSYTVCLSVGSAQGCADSVCQVIDVIPAEIIPPNVVTPNSDGVNDVLAF
                     KFLEFYPDNHLQVFDRWGNLIFDKEHYANDWDPQKYTDGTYYYILTVNTTKNYSGFFE
                     LIKGK"
     gene            complement(5243..6106)
                     /locus_tag="VFU15_01035"
     CDS             complement(5243..6106)
                     /locus_tag="VFU15_01035"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HEU4716382.1"
                     /translation="MEHDDIPQPPGARFSPGIKFWLVPSLMFACSGIYTLIASSSKFA
                     WMNAGPDDGYPVAYARWAEVIASVILFAIPAVVYANVFPPERFRWFRLDRKAGIAVTL
                     LAVLSLAIAVVGFDAVYNWIGTQIKDPALKEIQESGEKNTDWILSMPSAGDLFFCLFV
                     GALVPAFCEELFFRATFQQSLIEWNRKPHLSVWITAAFFSFMHFDIAGFPVIFASGLM
                     LGYAFVRTGSLRVNILMHFVFNSVSILESFIAERSAAFKSWEPGIPGAAISFGLAGAL
                     FFFFWRRTRTA"
     gene            6205..7197
                     /gene="dusB"
                     /locus_tag="VFU15_01040"
     CDS             6205..7197
                     /gene="dusB"
                     /locus_tag="VFU15_01040"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013454881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEU4716383.1"
                     /translation="MPRIGNITLPAFPLLLAPMEDVSDPPFRYVCKQNGADLMYTEFI
                     SSEGLIRDAIKSRKKLDIFDYERPIGIQIFGGDEEAMAMSARIVEATQPDLLDINFGC
                     PVKKVVCKGAGAGVLKDIDLMVRLTKAVVKSTNLPVTVKTRLGWDDKTKNILEVAERL
                     QDIGVQALSIHGRTRMQMYKGEADWTLIGEVKNNPRITIPVFGNGDVDTPEKALEMKN
                     KFGVDGVMIGRASIGYPWIFSEIKHFLETGTHLPAPTIADRVNVCRTHLMKSVEWKGE
                     RLGIIEMRRHYANYFKGLPDFKDTRTKLVTTYDLAELNDTLDKVKETYVENVVV"
BASE COUNT         1810 a         1963 c         2103 g         1594 t
ORIGIN      
        1 ctggccgcag cgcgggattg ctgcctgttc tggcggtctg ccttaatctg cttgtatttt
       61 gcgatctggt cgggcgtgag aaccgcaagt acatcggttt cgttctggtc acgcactttc
      121 tggatgtctt cttttttctg ctggtcggtc ttcgtcgcat cattgttgat ctggtcgatc
      181 gactggatgc gggtcaggaa caatgcttca acgcttgttg tctgctcgtc ggagagaccc
      241 agcatttttt gcatctgcat ggcttgcgta tgagccttct gctgtggagt gggcgcatca
      301 gcaggggcag cagaagtagc tgattgcgcg cgtactgctg caaaagaaag aaggatgccg
      361 aataagagaa taattctttt catgtgtata gctttagtct agtaaaagta aatcataggt
      421 cccagaaaag caaaccccgt gccggccttt gtcgaaaaag aaaaaacgcc tttatggctg
      481 tttgcccccg gcataactac ctttgaaccc atggaatatt accgctacat catggccctc
      541 cacgtcattt tcgtggtcag ctggttcgcc gctcttttct atatgccgcg cctgctgatc
      601 tatcatgccg aagcggacca gaaacccgaa cccgaccgcg gcatcctgtc ggaacagctg
      661 aagatcatgc agaaacgcct ctggggcata attgcgtggc ccgcactgct gctgacgtgg
      721 gccatgggat tatggatgat gtacctgaac ccgtccctcc tttcccagcc ctggtttatc
      781 ctcaagctgc ttttcgtttc cggcctgacg ttgtatcatg tgcagacgca cgcgctttac
      841 atgaagcacc agcgcgacga aataccgtgg acgtccttca agctccgctt atggaatgaa
      901 gtggcgacca tctttctttt cgcgatcgtt ttcctcgtca ttcccaagca gaatacgggc
      961 tgggtgtggg caacgctcgg tcttatcggg tttgccgccg ccatgttcgc ggccgtttcc
     1021 atttaccgga aggcgcgcga aaaaaagaaa gaggagccgg atgcttaagc attagataac
     1081 cccgggttga ggaaaagttc aggtgcgggt aatattgcgt aaagacgtgt tcggttgctt
     1141 tgcgcaatgg atgaaccgac ggatttcaac gaaccgctta ctattctcat tgtagacgat
     1201 gaacccgata tccgggaatt catctcttat tttctttcgc acaaaggata ccgtgtcttt
     1261 gcagcttcca acggacgtga agctgtcgat atcgccaagc gtgtaattcc cgatctcgtc
     1321 atcctcgaca tactcatgcc cgaaatgaac ggctatgaaa cctgccgcgt gctccgctcc
     1381 acgcccgggc tcgagatgac gcgcatcctt ttcctcagcg cactttcgga atcctacgtc
     1441 ggggagatca gcgacaaact gaatgcggat ggattcatca gcaagcctat ccggatggga
     1501 atgctggtaa aaaaagtaga ggaacggatt cgcaggaccg ggtaggaaaa ttatttccgc
     1561 agtatccagg cttcgggaat atccctgcgt atctcagaaa gaaaacggtc cgcttcttcc
     1621 tttgtgcgga acgatcccac gcccacatgc gtcaggcggc tgcgcaggtt ctccaccacc
     1681 tcggcgtgaa aaccgcgctc ctgcaacgcc ttcctgaaat tatcagcgtt ctgcgaaacg
     1741 gcgaagcacc cggcgataac gaaataatga tcgtctgcag atgaagagcg cggaggcgcg
     1801 acattgctgt tgacagccga tgcgacgtaa agggaatcag gcagcacttt gtggatgtcg
     1861 acgatgatcg gcggcatttt ttcagacgat tcgatgcgcg ctattccacc ctggtcggga
     1921 acgagccggc tgttcccggt tgtatcagaa aacaacttcg ttttattgaa ccactgaacc
     1981 ggcttgtaca acgaaggttc gtgcctgaaa aacccgaatc ccgcgatgtc tttgctgtag
     2041 gaagcggtaa accaggtacc gatgaggaga agcggaagaa tgatcgaggc ggcaatggcc
     2101 agcttgcgga gcgggtaacc ggcagccgga acttcaggct tgggctccgg cgccgtgcgg
     2161 atgtcgcgga caaccggctt gcgcacttcg ggctcttttt ccttctcgcg gataaccggc
     2221 atcgactgga acgaagccag tccgaatgct tcgacaaggt aattcacatc cggctcctgc
     2281 tcgaaaccga tattcccttc ggggtcgacg gaaaacgtac cgatgtgcgg gagctccgtc
     2341 ttttttcctg cccggagcga ctgctggatc ccttccgcaa aagcggcaat ggccttcatc
     2401 gcttcatcga aagaatgacc cgattcttcc acgatcgtct gcgcaagcaa accgtcattc
     2461 tgctgaaggt gcttattgaa aacgatcctg cgcgaaggtg gatcgaacgt atggcgggcg
     2521 ggatggatct tcgccggaac ttctgtgcac acaaaagcgc ccagtccggg cacaacgacg
     2581 cagtcgtggc ggaaaagcag cgagcttatg tagtgggaaa tattgatctc cgggtccatc
     2641 gttaaaaaac aaaaatagcc aaaacgtatc caggggagga cttgtttttt tcaacggaat
     2701 aataacaatt tagaaaagag gagattaaaa gccgttatgt gcgaatgccg gcctgtatcc
     2761 ccgtcaatcg acgcttactt tcctttgatc agctcgaaaa acccgctgta atttttggtg
     2821 gtattgaccg tgagaatgta ataatacgtt ccgtcggtgt atttctgcgg atcccagtcg
     2881 ttggcgtagt gctctttgtc aaaaatgagg ttgccccagc ggtcgaaaac ctgcaggtgg
     2941 ttatccgggt aaaactcgag gaatttgaat gcgagaacat cattcacgcc gtcgctgttc
     3001 ggtgtaacca cgttgggcgg aatgatctcg gccgggatca cgtcgatcac ctggcaaacc
     3061 gaatcggcgc atccctgtgc agaaccaacg gaaaggcaaa cggtgtatga gccgggtgag
     3121 ctataggtgt aaaccgggtt ctgtaaagcg gaaacggaat tgtcgccgaa atcccagccc
     3181 cacgatgctc ctgcaccgga ataaaaagaa gactgatccg tgaagcttac cggaactccc
     3241 gcaatcgccg gcgaagagaa cgtgaacgca gccgaaggaa atggctgaac aacggcggga
     3301 aaagtgatcg tgtccgacgt acacccgttg atcgacgcaa atgcattgaa ggttatcgac
     3361 gtatccgccg tgccggtata gttaacagag ccgttcacat tcccgttgcc ggaaccggaa
     3421 gagttgtacc acgaaaccgt gctgcccgcc aggcaggaat tgagattgaa ctggtagggt
     3481 tcgccgggac agaccgtatc cgaaggcggc gaaacagtca gtaccggaac ttcatgaata
     3541 tgcagcggcc gtgaaggata gccggtcgca atgtttccgc acgcgtccac gacaacgggc
     3601 ggaggaacgg tgagcgtcgt gatcgtatcg caataggtgg gacaattcgg gatcgtcagc
     3661 gtgatctgct tgctgacgat ggcataatta catcccgcag gacttcccgc tgtaacgctc
     3721 ccggtcatcc acggatgcgt gatcgtgaac gggggaacgc cgtagtgaat gaaaacattg
     3781 cccgtgaacg tgcagacatt ggagcaaacc ggtgtcaccg gaaccgtaaa tccctgcgcg
     3841 ttcatttcga ccgtgtggcc gacgatgtat gcgctgaacg gccacgatgt cgtcacagga
     3901 tcatagcgga tgtacgttgt gttgcagccg gttcccggac ctgtgcgcga aaggtgcatg
     3961 gtgaggtaga acgtctgcgg tgagcaggac tgcgcatagc agcacgtaag cggactgcgc
     4021 aggttgaacg tatcgagata agcagtaccc ggcaggtttc cgttcggcgg accgatcgtg
     4081 aactgcggtg aagaaccgca gggagtggtg aaccacatcg cgccgtcgct catgatcgcg
     4141 gtggtgaacg gatcggcgta aaagctggat gttacggcaa ggctggtgac tgtgatctgc
     4201 cctggaatcg tgacgaggat gctgtccgta tgattggcgg gtgcaatgca cgaaggcatg
     4261 gatcctccgc tccacaatgc agtcggtccg atgaccagcg gatcgatcgt cacaggatag
     4321 ctgcgcacag cgtcgttcat ccacgaccgg ggaacgagaa tgtcgagcaa ttccatgtcc
     4381 gtgtgttcgt catagcggat aacgtatgtg ccgaacgtat actttccggc tgcatcgtaa
     4441 cacatcgccg ctttgatcgt tccgacaaca gcatcatcag actcgcggat gaccagcagg
     4501 tcgccagtcc agccgccgtt ctctttgcgt ccgcgcgact catcctcttt cagagtaaaa
     4561 ccatggtcga gtaaaaatcc ttccgttacg acgaagttgt ccgaattcac ctgctgcggc
     4621 tggcgcacga tgtagcttgt ttcgattcca ttgaggcgga aggcatattt ccggtccacg
     4681 ccgggaacca catcatgcat tgttcccgta tcaccgtcga acgcgatcgc ctgcggcgaa
     4741 gcattcacac cgttgatcac cgggctgatc ccgaaccgga aataatcgcc ttcgtctttt
     4801 ccataagaaa cataagcgct tccatcgttg aacaggaatg tgggaaacgg ctgggcggga
     4861 gcggtccatc cttccgtgat ctttttcgga gagaaatcga tgggctgcag tttgccgtta
     4921 acatcgtaat aattgatcgg gacggagctc tggtgtgcga tgtattgtcc gtccgcggtg
     4981 tgatacacgc tcgagaaaac cgttcgctgt gattcgtcct ccacccacgt tgaacggtcg
     5041 ggcaatgaag ttccgggaac gacaggatct gcgggggcga ctttcgccgc aaacggcggg
     5101 acatcctgtg cgtgcgccat tgtcatgaaa agtgcgcaga aaaaaacaag cccgtaaaac
     5161 ttcgcggaag tgcggacagc gttcataagt gatcagtttt gaaaaccagg ttaagccaga
     5221 attaaaaata gcaaaaaacg cgttacgcgg tccgggtcct gcgccagaaa aagaaaaaca
     5281 atgcgccggc aagcccgaac gagatggcgg ccccgggaat ccccggttcc cagcttttga
     5341 acgcagcgct gcgttccgcg atgaaggatt ccaggatgga aaccgaattg aagacaaaat
     5401 gcatgagaat gttcacacgc agcgaacctg tccgcacaaa ggcgtaaccg agcataagtc
     5461 cggaagcgaa aatgaccggg aaacccgcga tgtcgaaatg catgaaactg aaaaaagcag
     5521 cagtgatcca gacggaaaga tgcggcttgc ggttccattc gatgagcgac tgctggaagg
     5581 tggcgcggaa gaacagttcc tcgcagaatg ccggtaccag cgcaccgacg aacaggcaga
     5641 agaacaggtc gccggcggaa ggcatgctga gtatccagtc tgtgttcttt tcaccggatt
     5701 cctgtatttc cttcagcgcc ggatctttga tctgcgtgcc gatccagttg taaaccgcat
     5761 caaaaccgac gacagcgatc gcaagggaaa gcacggccag cagtgtgaca gcgatgcccg
     5821 ctttacggtc gagacggaac cagcggaaac gttcgggcgg aaaaacattc gcataaacga
     5881 cagccggaat tgcgaaaagg atcaccgatg cgatcacttc cgcccagcgc gcgtacgcaa
     5941 cgggataacc gtcgtccggg cctgcattca tccaggcgaa tttcgaagag cttgcgatca
     6001 gcgtatagat cccggagcac gcgaacatca gcgaaggcac gagccagaat tttattcccg
     6061 gtgaaaagcg cgcccccggc ggctgcggta tatcgtcgtg ttccacttct tcgctgcgtt
     6121 ttggctaaat ttgcgcaaaa ataggttgca aaactgaaaa gccggaaagt acagaagctt
     6181 tcaccgcaac tttccaactg tattatgccc cgcatcggca acataacgct tcccgccttc
     6241 ccgcttcttc tcgcaccgat ggaggacgtg agcgatccgc cgttccgtta cgtctgcaag
     6301 cagaacggag ccgatctcat gtacacggaa ttcatctctt cggaagggtt gatccgcgac
     6361 gcgatcaaaa gccggaagaa gctcgacatt ttcgattacg aacgtccgat cgggatacag
     6421 atcttcggcg gcgacgaaga agcgatggcc atgtcggcgc gcatcgtgga ggcgacacaa
     6481 cccgatctgc tcgacatcaa cttcggctgc ccggtgaaga aggtcgtgtg caaaggcgca
     6541 ggcgcgggcg tgctgaaaga catcgacctc atggtgcggc tgacgaaagc ggtggtgaag
     6601 tcaacgaacc ttcccgtaac ggtgaaaacg cgcctgggat gggacgataa aacgaaaaat
     6661 attctcgagg tcgcagaacg tttgcaggac atcggcgtgc aggcgctgtc gatccacggg
     6721 cgcacgcgca tgcagatgta caagggtgaa gccgactgga cactcatcgg cgaagtgaag
     6781 aacaaccccc gcatcaccat tcccgttttc ggcaacggcg atgtcgacac gccggaaaaa
     6841 gcgctggaga tgaaaaacaa attcggcgtc gacggggtga tgatcggtcg cgccagcatc
     6901 ggctatccct ggatattcag cgagatcaaa catttcctcg aaacgggaac gcatcttcct
     6961 gctcctacga tcgccgaccg cgtgaacgtg tgccgcacgc atttgatgaa atctgtcgaa
     7021 tggaaaggcg aacggctcgg catcatcgag atgcgccgtc attatgcgaa ttatttcaag
     7081 ggacttcccg atttcaaaga cacgcgcacg aaacttgtta cgacgtacga tctcgccgag
     7141 ctgaatgaca cgctggacaa ggtgaaggag acgtatgtgg agaatgtggt ggtgtaggtc
     7201 tcgactcgcg ctcgacctga caaggggatc gactggcgct cgacctgacg aagggaaacg
     7261 attggcgctc gactgacagg tttcgactcg cgctcgacct gacaagggga tcgactggcg
     7321 ctcgactgac agtggtctcg actcgcgctc gacctgacag aaaaaaatcg actcgcgctc
     7381 gacctgacga agggaaacga ttggcgctcg actgacaggt ctcgactcgc gctcgacctg
     7441 acaaggggat cgactggcgc tcgacctgac
//
LOCUS       DASTSF010000038         9970 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1756094,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000038 DASTSF010000000
VERSION     DASTSF010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 9970)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9970)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9970
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1756094"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="VFU15_01045"
     CDS             <1..262
                     /locus_tag="VFU15_01045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716384.1"
                     /translation="TIHDAGRTGAAKITFDKTYPVPASDTMAPLLCSDTSKIALFTSG
                     VSLDWQQTPRKRIELRKSGTTLIDDLPNPDQRKPGTTIYVYV"
     gene            287..2287
                     /locus_tag="VFU15_01050"
     CDS             287..2287
                     /locus_tag="VFU15_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015903217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath C-terminal domain-containing
                     protein"
                     /protein_id="HEU4716385.1"
                     /translation="MTTYKTPDVYVEEISIFPPSVAEVETAIPAFIGYTEIADNAGTS
                     LLMKPTRISSLLEYRTYFGGGPSPDITEVKLNDDNTFKSVTQNADYYMFDSLQLFYAN
                     GGGDCYIVSVGLYGDTIITGDDTIDPPTGILGGLKVVEKFDEPTILLFPDAVNISGDD
                     LYSKIQPAALAQCNKLMDRVAVFDLHEDDPKGTTFRDKVGINYLKYGMAYTPWLDVNL
                     GKTIMYRDLLDSSTYASKLKKTGVVPLAALTNSTDIQALITNLESAIKDVDTVASDVS
                     TNLLSSGTFDSVRSHYESLKGDYNAATDDAGHEAKLKLLIKFLYDVAVQVDDWTDSST
                     GLLYSKNRDDISTAIDSILRGSYSKLRSYEKELHDDVAGYTAASPSSTPANVSWQTDP
                     LDALTNIFTATPASLGVVSGTTDFDKVDSVFKLIDDRFEEISAAFNTILNGVSAYETS
                     LDKSLNDSFPTYKNIISGIGSSFTSMPPSGAIAGVYAETDRDRGVWKAPANVSLSSVV
                     GPATTFTASELDALNIDVNAGKSINAIRAFTGKGTLVWGARTLAGNDNEWRYIPVRRF
                     FNMVEESVKKSTYWAVFEPNSANTWVKVKGMIENYLTLKWKDGALAGAKPDEAFFVKI
                     GLGTTMTAQDILEGRMNVEIGMAVVRPAEFIILKFSHLLQQS"
     gene            2342..2773
                     /locus_tag="VFU15_01055"
     CDS             2342..2773
                     /locus_tag="VFU15_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015903216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEU4716386.1"
                     /translation="MAKYPSLVLHFRVEWGGTSIAFTEVGGLTQEAQAVEYRTGDSPD
                     YSSVKMPGMRKYNNITLKRGITASDNDFFKWLNTIKLNDVERRDITVSLLNEKHEPVM
                     VWKAHNAFPVKVEGPGLKASGNEVAIESIELAHEGLTIQND"
     gene            2789..3244
                     /locus_tag="VFU15_01060"
     CDS             2789..3244
                     /locus_tag="VFU15_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEU4716387.1"
                     /translation="MSYYPPLGFHFRVEFLDTSGAQKFSETSFQEVSGLTVEIGTEEL
                     SEGGVNDHSHRLPGKVKYGNLVLKRGMFTDSKLVDWIVKAVENYSFDPLNITVSLLNA
                     DHQPLMSWHFFRAWPVKWVTSDFKAQDNGIVVESFELAYKNFTCRKPNS"
     gene            3246..3419
                     /locus_tag="VFU15_01065"
     CDS             3246..3419
                     /locus_tag="VFU15_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014022731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5908 family protein"
                     /protein_id="HEU4716388.1"
                     /translation="MAIQVNEIIIRATVSEGGEAQQGHHSRKKSDDPTGKDEIIAECV
                     EQVMEILNREIKR"
     gene            3424..4107
                     /locus_tag="VFU15_01070"
     CDS             3424..4107
                     /locus_tag="VFU15_01070"
                     /inference="COORDINATES: protein motif:HMM:NF039279.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEU4716389.1"
                     /translation="MADAITPSTGELKKLTIRTYKDANYQTPGDPDKFTAMFNPASYA
                     EKYEVEYKAEQAHGNSASSMKFGSIKPKDYNFEFMIDGTGTVTDKVNVTDVIDLFLKA
                     CVQIVSDTHRPYFLLINWGNLNTKVVLKSADITYNLFDNDGKPIRAKIAASFTENIED
                     TLRVRQEGANSPDLTHYRQVKVGGKLPVMVYKEYDSHLQYLSVARANNLDNFRRLSNG
                     SQLILPPIK"
     gene            4120..5868
                     /gene="vgrG"
                     /locus_tag="VFU15_01075"
     CDS             4120..5868
                     /gene="vgrG"
                     /locus_tag="VFU15_01075"
                     /inference="COORDINATES: protein motif:HMM:TIGR01646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HEU4716390.1"
                     /translation="MSDNDRTIPTPQPSDLPSLTILVDGTAISTEYKVASVDVTKVFN
                     KLSYARIAIYDGDVAKSNFEVSNKDDFKPGKEIEVQAGYHNDNKTIFKGIVTSHSLRI
                     RKEKGFYLIIEARDKSVKMTAGRNCAYFYNAKDSEIIEQLAGNAGVDKDVEATDVQHK
                     EMVQYHVTDWDFILSRAEVNGKFVLTDDNKLIVKKPDTSSDAVLSIEFGSTIMDFEGE
                     IDATTQFSTVKSHAWNYTEQELVESEGSDPPVSDNGNLTSSDLASVLGVAEFSLRHPG
                     KFEESELKSWTDAQIVRNKLARVRGRAKFQGYADVKPGMMIELKGVGDRFNGKAFVSG
                     VRHQVNTANWTTDVQMGFSNKWFHDAPDIVDAKSAGLVPGVNGLHIAIVTKLESDPDG
                     EDRIQVKMPLIDNNADGTWARIATLDAGKERGSFFRPEIGDEVILGFLNDDPRNPIVL
                     GMVNSKKLPAPLQAADANNEKGFVTREKLKLIFNDDKKSVTVETPGGQSIVIDDDSGK
                     ITIKDKNKNTVEMSSNGISMDSASDFKIKAKGDISIEGTGVTVKASGSLGLQGATSEL
                     KADGNTTVKGAMVAIN"
     gene            5885..6175
                     /locus_tag="VFU15_01080"
     CDS             5885..6175
                     /locus_tag="VFU15_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020160106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HEU4716391.1"
                     /translation="MGSAARTGDMHTCPMVTGVVPHVGGPVMPPGCPTVTIGGMPAAR
                     VGDMCTCTGPPDVIAMGSTTVTIGGMPAARMGDPTAHGGSITVGCPTVTIGG"
     gene            6213..6638
                     /locus_tag="VFU15_01085"
     CDS             6213..6638
                     /locus_tag="VFU15_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HEU4716392.1"
                     /translation="MKDDQASQSFLGTGWGFPVAFNNVSGDVGMTSDVEDIYQSLEIL
                     LSTAISERLMQPKYGCNLHDVLFEPISTSLLSFLKGLITDAILYFEPRIRLNKVTIST
                     DNYLEGLLPIEIDFTVSATNSRYNFVYPFYINEASSLNQ"
     gene            6659..>9970
                     /locus_tag="VFU15_01090"
     CDS             6659..>9970
                     /locus_tag="VFU15_01090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716393.1"
                     /translation="MNPDTNDTTGVQQPDPCGCETLHPLERDGTSQAQRLLAALDPSS
                     VSVDERDLGDLLVFARDYAKLLNYYKSSGEDVEADGDWSVFLKHDITVLVAIVSKTDI
                     KTMRDTFRAKLKTISASSDVHAPIDTLNDFRDLLSWISSWYADSDSITLFHKELNLYF
                     QSMMLDGYSKLIAIDTLGGFNVFAGFTFPSNPEWQALADAADPYTDTSLYTSADPLVN
                     NEAAARIMQTVFEKYAAALQIIVNRTPDYLAESIAQYPYHKAHLGLFITFLELFAYAQ
                     KHLNELTERHLDFFYQEVLQLTPEAAVADKVHVIFELAKNASDTFKVSQDTLLKAGKD
                     ALKKELLYGTDEDIVLNKAKVAGFRNLFIDNFSAGSITANGVYASPVANSSDGNGAAL
                     DKTIPQWQAFGSSQQGIGTASHTMPDARTGFAISSPQLFLSEGTRIVTLEIMFDPAHA
                     FSGTAAEISELQNADYYQFSFTGAKGWLTPDSTQIIYSNTTHSLKFIFTLNAKLAAVT
                     AYSSAVHAGTFNTSNPILKVELLNLQQADTPYWKNVYTLLDEVAVVSMDLTVDVKGMQ
                     NLTLHNEASKLDPSKPFMPFGSQPVIGSPFYVGSDEVFFKDLSSLTLNVLWHGAPSDF
                     TDQYANYLAYDNNSSTDTHQIDVDNDDFMATGSLLHDGTWSAEMDFLLNNSSSKKDRL
                     FDDDAQDAVQYNFSGISSDRANSSDVFLNTPLGSTRGYLRLTLGNKDFLHTKYAQSLA
                     IQALKTTGGKIPLPPYTPAIKTFSADYISTQTLDSTQDQFYHLQPFGEQLFSEFPVDD
                     DSKLYLLPQFRASAYNYSTGETDPLMKQTGMLFIAVEDLVPQKSLSLLIQVVEDSGNR
                     NAEAPAITWSYLQNNEWIDLTAQQVISDTSNGFQTSGIVELDIPDTATNDNTILPAGY
                     HWLRAAVTKQDNKDPSALCNVLDIIAQAVQASFRDNGNDPQHLATALAAGTIAKLSEP
                     LAEVKSVSQPYASFGGKVAEEGDDYYRRVSERLRHKGRAITIWDYERLVLENFPSIYK
                     VKCISHTRDVTGEPDCYLELAPGNVCLTVVSNLRNQGTQADLLKPSTSINTRGEIKTF
                     LQKRASKFAKITVINP"
BASE COUNT         2582 a         2832 c         2630 g         1926 t
ORIGIN      
        1 caccatacac gatgcaggcc ggacgggcgc ggcaaagatc accttcgaca aaacctatcc
       61 cgttcccgct tcggatacga tggctccgct gctctgcagc gacacgagca agatcgcttt
      121 gttcacctcg ggcgtttcgc tcgactggca gcagacgccg cgaaagcgga tcgagctgcg
      181 caaaagcgga actacgctta tcgatgactt accgaatccc gaccagcgca agcccgggac
      241 cactatctat gtctatgtct aaaaccaaaa acaaaccttc tttcctatga caacctacaa
      301 aacgcctgac gtttatgtcg aggagatctc gatcttccct ccttccgtgg cggaggtgga
      361 aaccgctatt cccgcattta tcggctacac cgaaattgcg gataatgcgg gcacttccct
      421 gctgatgaaa cccacccgga tcagctcgct gcttgaatac cggacctatt tcggcggcgg
      481 accttcgccg gacattaccg aagtaaagct gaatgacgac aacacattca aaagcgtcac
      541 gcagaatgcg gactattaca tgttcgacag cctccagctt ttctacgcga acgggggcgg
      601 cgactgctac atcgtttcgg tgggccttta cggcgatacg atcatcacgg gcgatgacac
      661 gatcgatccc ccgacgggaa ttctcggcgg actgaaagtc gttgaaaaat tcgacgagcc
      721 caccattctt cttttccctg acgccgtgaa tatttccggc gatgatcttt acagcaagat
      781 ccagccggcc gctcttgcgc aatgcaacaa gctcatggat cgcgtggcgg tgttcgatct
      841 gcatgaagac gatccgaaag gaacgacgtt ccgggacaaa gtcggcatca actacctcaa
      901 gtacggaatg gcttacacgc cgtggctcga cgtgaacctc ggcaaaacga tcatgtaccg
      961 cgacctgctc gattcttcca cctatgcgag caagctgaaa aaaaccggcg tggtgccgct
     1021 cgctgcgctc acgaacagca ccgacatcca ggcgctgatc acgaacctcg aaagcgcgat
     1081 caaggatgtg gatacggttg cttccgatgt gagcaccaat cttctttcat cggggacttt
     1141 cgattccgtc cgttcgcatt acgaatcgct gaaaggcgat tacaacgcag ccaccgatga
     1201 cgcaggacac gaagcgaagc tcaagctcct gatcaagttc ctgtatgatg tcgccgtgca
     1261 ggtggatgac tggaccgact cctcgaccgg gcttttgtat tcgaaaaacc gcgatgatat
     1321 ttccaccgcg atcgattcca tcctgcgcgg ttcgtattcc aagctgcgtt cgtatgaaaa
     1381 agagctgcac gacgatgtcg ccggttatac ggcagcttca ccttcttcca ctcccgcgaa
     1441 cgtcagctgg cagaccgatc ctttggacgc attaacgaac attttcacgg ccacgcccgc
     1501 atcgctcggc gtggtttccg gcacaaccga tttcgacaaa gtggacagcg tattcaagct
     1561 catcgacgac cgcttcgaag agatcagcgc agcgttcaac acgatcctca acggcgtgag
     1621 cgcatacgaa acgagtctcg acaagagtct caacgattcg ttccccacgt ataaaaacat
     1681 catcagcggg atcggatcga gcttcacttc catgccgccg agcggcgcga tcgccggagt
     1741 gtatgcggaa accgaccgtg accgcggcgt atggaaagcg cccgcgaatg tcagtctctc
     1801 ttccgtggta ggtcctgcca ccacattcac tgcatccgag ctcgatgcgc tgaacatcga
     1861 cgtgaacgcc gggaaatcga tcaacgcgat ccgcgcattc acgggcaagg gcacgctcgt
     1921 atggggcgca cgcacgcttg ccggcaacga caacgaatgg cgttacatcc cggtgcgccg
     1981 cttcttcaac atggtggaag agtcggtgaa aaaatcgacc tactgggccg tgttcgaacc
     2041 gaacagcgcg aacacgtggg tgaaagtgaa aggcatgatc gaaaattacc tgacgctgaa
     2101 atggaaagac ggcgcgctgg ccggtgcaaa acccgacgaa gcctttttcg tgaagatcgg
     2161 tctcggcacc acgatgacgg cgcaggatat tctcgaaggg cgcatgaacg tggagatcgg
     2221 catggcggtg gtacgcccgg cggaattcat catcctgaaa ttctcgcacc tgctccagca
     2281 gtcgtaagca tcaccggaaa aattcattca acgtttcaaa ccgacttaaa gaacaatcca
     2341 aatggcaaaa tatccctcac tggttcttca cttccgcgtc gaatggggcg gtaccagcat
     2401 cgcattcacc gaagtgggcg gcctgacgca ggaagcgcag gctgtggaat accgcaccgg
     2461 cgacagtccc gactattctt ccgtgaagat gccgggcatg cgcaaataca acaacatcac
     2521 gctcaagcgg ggcatcaccg catcggacaa tgattttttc aaatggctga acacgatcaa
     2581 gctgaacgac gtggagcgcc gcgacatcac ggtcagcctg ctcaatgaaa aacacgagcc
     2641 ggtgatggtg tggaaagcgc acaacgcgtt cccggtgaaa gtggaaggtc ccggcctgaa
     2701 agcgagcggc aatgaagtcg ccatcgaatc catcgagctc gcgcacgaag gactgaccat
     2761 acagaacgat taataaaaaa ccaccggtat gtcctattat cctccgctgg ggttccattt
     2821 ccgcgtggag ttcctcgaca cgagcggcgc gcagaaattc agcgaaactt ctttccagga
     2881 agtttccggt ctcactgtgg agatcggcac ggaggaatta tccgaaggcg gtgtgaacga
     2941 tcattcgcac cgcctgccgg ggaaagtgaa atacggcaac ctcgtgctca agcgcggcat
     3001 gttcacggat tcgaagctgg tcgactggat cgtcaaggcc gtcgagaatt attccttcga
     3061 tccgctgaac atcaccgttt cattgctgaa cgcggatcac cagccgctga tgagctggca
     3121 ttttttccgc gcgtggccgg tgaaatgggt gacgtcggat ttcaaagcgc aggacaacgg
     3181 gatcgtggtg gaatcgttcg agctggctta caaaaatttc acctgcagaa aaccgaattc
     3241 ataacatggc catccaggta aacgagatca tcatccgcgc caccgtcagc gaaggcggtg
     3301 aagcgcagca gggacatcat tcacgaaaaa aatccgacga tcccacggga aaagacgaga
     3361 tcatcgcgga atgcgtggaa caggtgatgg aaatcctgaa ccgggaaatc aaacgctgaa
     3421 cgaatggcag atgcaatcac accgtcgacg ggagagctga agaagctcac catccgcacg
     3481 tacaaagacg ccaattacca gacgccgggc gatcccgaca agttcacggc tatgttcaac
     3541 cccgcttcgt acgcggaaaa atacgaagtg gaatacaagg cggagcaggc gcacggaaat
     3601 tcagcgagct cgatgaagtt cggcagcatc aagccgaagg attacaattt cgaattcatg
     3661 atcgacggaa cgggaaccgt cacggacaaa gtgaatgtca ccgatgtcat cgacctgttc
     3721 ctgaaagcgt gcgtgcagat cgtcagcgat acgcaccgcc cgtatttcct gctgatcaac
     3781 tggggaaacc tgaacacgaa agtggtgctg aagagcgccg acatcaccta caaccttttt
     3841 gacaacgacg gtaaaccgat ccgcgcgaag atcgcggctt cgttcacgga aaacatcgag
     3901 gacacgctgc gcgtccggca ggaaggcgcc aactcgcctg acctcacgca ttaccggcag
     3961 gtgaaagtcg gcggcaagct gccggtcatg gtttacaaag aatacgacag ccacctgcaa
     4021 tatctttccg tggcgcgcgc caacaacctc gataatttcc ggcgtctttc caacggatcg
     4081 cagctcatcc ttcccccgat caaataacat gcgtgcttta tgagcgacaa cgaccgtacc
     4141 ataccgacac cgcagccgtc cgatctgccc agcctcacga tcctcgtgga cggtacggcg
     4201 atcagcacgg aatacaaggt cgcctctgtc gatgtaacca aggtcttcaa caagctgtcg
     4261 tatgcacgca tcgcgatcta cgacggcgac gtggcgaaga gcaatttcga agtgagcaac
     4321 aaagatgatt tcaaacccgg gaaagagatc gaagttcagg ccggttacca caacgacaac
     4381 aaaacaatct tcaaaggaat cgtcaccagc cacagcctgc gcatccggaa agaaaaagga
     4441 ttttacctca tcatcgaagc gcgtgataag tccgtgaaga tgacggcggg aaggaactgc
     4501 gcctatttct acaatgcaaa agacagcgag atcatcgagc agcttgcggg aaatgcgggc
     4561 gtggacaagg acgtggaagc gaccgatgtg cagcacaagg aaatggtgca gtaccacgtc
     4621 accgactggg atttcatcct ttcgcgcgcc gaagtgaatg gaaaattcgt gcttaccgac
     4681 gacaacaagc tgatcgtaaa aaaacctgac acgagcagcg acgctgtcct gagcatcgag
     4741 ttcggctcga cgatcatgga tttcgaaggc gagatcgacg cgacaacgca attcagcacc
     4801 gtgaaaagtc acgcgtggaa ttacaccgag caggagctgg tggaatcaga aggcagcgat
     4861 cctcctgttt cggacaatgg aaatctcacc tcgtccgacc tcgcatccgt gctcggcgtg
     4921 gcggaatttt cactccgtca cccgggaaaa ttcgaagaga gcgagctgaa aagctggacg
     4981 gatgcgcaga tcgtgcgcaa caagctggcg cgcgtgcgtg gccgtgcgaa attccagggc
     5041 tacgccgacg tgaagccggg catgatgatc gagctgaaag gtgtaggaga ccgtttcaac
     5101 gggaaagcgt ttgtcagcgg cgtgcgtcac caggtcaaca cagcgaactg gacgacggac
     5161 gtgcagatgg gattttcgaa caaatggttc catgatgcac cggacatcgt cgacgcgaaa
     5221 tcggcaggac ttgttcccgg cgtgaacggg ttgcacatcg ccatcgtgac aaaactggaa
     5281 agcgatcctg acggggaaga ccgcatccag gtgaagatgc cgctgatcga caacaacgcc
     5341 gacggaacgt gggcgcgcat cgcaacgctc gatgcgggaa aagagcgcgg atcatttttc
     5401 cgtcccgaga tcggcgacga agtgatcctc ggttttctca acgacgatcc gcgcaacccg
     5461 atcgtgctcg gcatggtgaa cagcaagaag cttcccgcac cgctgcaggc agccgatgcg
     5521 aacaacgaaa aaggatttgt cacgcgtgaa aagctgaagc tcattttcaa cgacgataaa
     5581 aaatcagtca cggtggaaac accgggcgga caaagcatcg tcattgacga cgacagcgga
     5641 aagatcacga tcaaggacaa aaacaagaac acggttgaaa tgagctcgaa cggaatttcg
     5701 atggacagcg catccgattt caagatcaaa gccaagggtg acatttccat cgaaggaaca
     5761 ggcgttacgg tgaaggcaag cggaagtctc ggcttgcagg gcgccacatc cgagctgaaa
     5821 gcggacggca acaccacggt aaaaggagcc atggtggcga tcaactgata actgaaaaat
     5881 aaaaatggga tcagcagcaa gaaccggtga tatgcatacc tgcccgatgg tgacaggagt
     5941 cgttccgcat gtaggcggac ctgtaatgcc gccgggatgt cctacggtca cgatcggcgg
     6001 aatgccggcc gcgcgtgtgg gtgacatgtg cacgtgcaca ggaccgccgg atgtcattgc
     6061 aatgggatcg accacagtga cgatcggcgg aatgccggct gcacgaatgg gagatccgac
     6121 tgcgcacggc ggaagcatca ccgtcggttg tcccaccgtc accatcggcg gataaaaaaa
     6181 caggaacgaa caaattcatc caggaaccca ttatgaaaga cgaccaggca tcgcaatcat
     6241 ttctcggcac cggctgggga tttcccgttg cgttcaacaa cgtttcagga gacgtgggca
     6301 tgacatcgga tgtcgaagac atttaccaga gcctcgagat cctgctgagc acggcgatca
     6361 gtgaacggct catgcagccg aaatacggat gcaacctgca cgatgtgctc ttcgaaccga
     6421 taagcacgtc gcttctctca ttcctgaaag gacttatcac cgacgcgatc ctgtatttcg
     6481 agccgcgcat ccgcctgaac aaagtcacga tcagcaccga caactacctc gaaggattgc
     6541 tgccgatcga gatcgatttc acggtttccg caaccaactc gcggtacaat ttcgtttatc
     6601 ctttttacat caatgaagca agcagcctga accagtaagc aggcgaagaa caatacgcat
     6661 gaatccagac acaaacgata caaccggcgt tcagcaaccg gatccctgcg gatgcgaaac
     6721 actgcatccg ctggagcgcg acggcacgag ccaggcgcag cgcctgctcg cagcgctcga
     6781 tccttcctcg gtctccgtcg acgaacgcga cctgggcgac ctgcttgtgt ttgcgcggga
     6841 ttacgcgaag ctgctcaatt attacaaatc ttccggcgag gatgtggaag cggacggaga
     6901 ctggagcgta ttcctgaagc acgacatcac cgttcttgtg gcgatcgttt cgaagacaga
     6961 tattaaaacg atgcgtgata cgttccgcgc aaagctgaaa acgatctccg cttcttccga
     7021 cgtccacgca cccatcgata cgctgaatga tttccgcgac ctgctttcct ggatcagcag
     7081 ctggtacgcc gacagcgaca gcatcacgct ttttcacaaa gagctgaacc tctatttcca
     7141 gtcgatgatg ctcgacggct acagcaagct gatcgcgatc gacacactcg gcgggttcaa
     7201 cgtattcgcg ggattcacgt tcccgtcgaa ccccgaatgg caggcgctgg ccgatgctgc
     7261 cgatccgtat accgacacgt cgctctacac atccgccgat ccgctggtca ataatgaagc
     7321 ggcagcgcgc atcatgcaga ccgtatttga aaaatacgca gcagcgctgc agatcatcgt
     7381 caaccgcacg cccgattatc ttgcggaatc catcgcgcag tatccctacc acaaagcgca
     7441 tctcgggctg ttcatcacgt tcctcgaatt gttcgcgtat gcccagaagc atctcaacga
     7501 actgacggag cgccacctcg atttctttta ccaggaagtg ctgcagctta cgcccgaggc
     7561 tgcggtcgcc gataaagtgc acgtgatctt cgaactggcg aagaacgcat cggatacctt
     7621 caaagtttcg caggacacgc tgctgaaagc cggaaaagac gcgctgaaaa aagagctgct
     7681 ttacggaacg gatgaagaca tcgtactgaa caaagcgaaa gtggcgggat tcaggaacct
     7741 tttcattgat aatttctccg caggctccat aacagcgaac ggcgtgtacg catcacctgt
     7801 cgcgaactct tccgacggga acggcgccgc gctcgacaag acgatcccgc aatggcaggc
     7861 gttcggttct tcgcagcagg gcatcgggac cgcgtcgcac accatgcccg atgcgcgcac
     7921 cgggttcgcc atttcttccc cgcaattatt tctcagcgaa ggaacaagaa tcgtaacgct
     7981 ggagatcatg ttcgatccgg cgcatgcatt cagcggaaca gccgcagaaa taagcgagct
     8041 gcagaacgcc gattactacc aattctcctt caccggcgcc aagggatggc tcacaccgga
     8101 ttcaacgcag atcatataca gcaacaccac acattcgctc aaattcatat ttacgctcaa
     8161 cgcgaaactg gctgcggtca ccgcatacag cagcgcagtg catgcaggga cattcaatac
     8221 gtcgaatccc attctaaaag tcgagctgtt gaacttacag caggcggaca caccttactg
     8281 gaaaaatgta tacacgctgc tggatgaagt agctgttgtc tcgatggacc tcaccgttga
     8341 tgtgaaaggg atgcagaacc tcacgctgca caacgaagcg tcgaagctgg atccgtcgaa
     8401 accattcatg cctttcggtt cgcagcccgt gatcggttcc ccgttctacg tcggcagcga
     8461 cgaagtgttt ttcaaagatc tttcttcact tacgctgaat gttttgtggc acggcgcacc
     8521 ctctgatttc accgatcagt acgctaatta cctggcttac gacaacaact catcgaccga
     8581 cacgcaccag atcgacgttg acaatgacga tttcatggcc accggttccc ttctgcacga
     8641 tggtacgtgg agcgccgaga tggattttct cctgaataat tcttcctcca aaaaggacag
     8701 gctttttgat gatgatgcgc aggatgccgt acaatacaat ttctccggga tctccagcga
     8761 ccgcgccaac tcatccgatg tttttctcaa tacaccactt ggctccacgc ggggttacct
     8821 gcggctgacg ctcggcaaca aggatttcct gcacaccaaa tacgcacagt cgctggcgat
     8881 acaggcgctg aaaacaaccg gcggaaagat cccgcttccc ccctacactc ccgccatcaa
     8941 aactttttct gcagattaca tttccacgca aacgctggac agcacgcagg accagttcta
     9001 tcatcttcag ccgttcggcg agcagctctt cagcgaattt cccgttgacg acgacagcaa
     9061 gctttattta ctcccgcaat tcagggcaag cgcttacaac tactcgaccg gggaaacgga
     9121 tccgctcatg aagcagacgg gaatgctgtt catcgccgtg gaagatcttg ttccgcagaa
     9181 aagtctttcg ctgctgatcc aggtggtgga agacagcggc aaccgcaatg cggaagcgcc
     9241 cgcgatcaca tggagctacc tgcagaacaa cgaatggatc gatctcaccg cgcagcaggt
     9301 gatcagcgac acctcgaacg gtttccagac gagcggcatc gtagaactcg acatcccgga
     9361 cacggcgacc aatgacaata ccatactgcc cgcaggttat cactggctgc gcgccgccgt
     9421 gacgaagcag gacaacaaag atccttccgc gctgtgcaac gtgctcgaca ttatcgcaca
     9481 ggccgtgcag gcaagtttcc gcgacaacgg gaacgatccg cagcatctcg ccaccgcgct
     9541 ggctgcggga acgattgcca agctgtcgga accgctggct gaagtgaaat ccgtcagcca
     9601 gccgtacgct tctttcggcg gaaaagtagc ggaagaaggc gacgattatt accgccgtgt
     9661 cagcgagcgg cttcgtcaca aaggacgtgc gatcaccatc tgggattacg aacgtctcgt
     9721 gctcgaaaat tttccgtcga tctacaaagt gaaatgcatc agccacacgc gtgacgtaac
     9781 cggcgaaccg gactgttatc tcgagctggc gcccggcaac gtttgcctta cggtcgtttc
     9841 caatctccgc aaccagggaa cgcaggccga tctgctcaag ccctcgacga gcatcaatac
     9901 gcgcggagag atcaaaacat tcctgcagaa acgtgcgtcg aagtttgcga agatcactgt
     9961 gatcaatccc
//
LOCUS       DASTSF010000039         7053 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_192297,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000039 DASTSF010000000
VERSION     DASTSF010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 7053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7053
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192297"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="VFU15_01095"
     CDS             <1..220
                     /locus_tag="VFU15_01095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716394.1"
                     /translation="KDVDIASQFTDPFTFEGPTATSTDMDMEMPDGTIKKVYMTTTDC
                     DLSVQEKQIVVPCEVNFTLTPPPAPPVK"
     gene            complement(224..1528)
                     /locus_tag="VFU15_01100"
     CDS             complement(224..1528)
                     /locus_tag="VFU15_01100"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015807905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HEU4716395.1"
                     /translation="MKERVSVFWFRRDLRLNDNRGLSAALDSGFPVLPVFIFDTDILG
                     KLDDKKDRRVTFIYRNLQKINEQLAKNGSRLFVFYGKPVEIWKELPGKFNVASAFWNG
                     DYEPYARRRDEDVAAVLAEKGIPAFSFKDQVVFEKSEIVKDDGMPYTVFTPYKNKWRR
                     SIAEKDMQEFPSEKRAGNFLRTDFVPLPPLSSFGFEETEMDCSVPPLNDEMLRTYSSL
                     RDFPARRATSHLSVHLRFGTVSIRALFRRALAVSETWTNELIWREFFMQVLWHFPHVE
                     KDSFRKEYDRIAWRNNEEEFERWKNGMTGFPLVDAGMRELNETGFMHNRVRMMTASFL
                     VKHLLIDWRWGEAYFASKLLDFDLAANNGNWQWAAGTGTDAAPYFRIFNPHEQQKKFD
                     PEFEYVKKWVKEFGTEKYPLPLIDHAAARGRCLAAYSAAVKD"
     gene            complement(1536..1970)
                     /locus_tag="VFU15_01105"
     CDS             complement(1536..1970)
                     /locus_tag="VFU15_01105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716396.1"
                     /translation="MPGTVPIYKIIPPAIASAGVYFFTTDSGLQYEVRFGRRQDNILH
                     ATIVFGVINEEFDGEEYVVTNRGEVFRVMNTIVQIVKMFMAEHEKIMCYEFTGIPKEG
                     EEESKASQRTLLYKRFLPKIFESGWEFNFSGNSAIVTRRQRH"
     gene            2277..3947
                     /locus_tag="VFU15_01110"
     CDS             2277..3947
                     /locus_tag="VFU15_01110"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU4716397.1"
                     /translation="MKNVLALVLLLFLAAPVSAQNKKAKKFLAAGNAASAAKNYDAAI
                     DNYTKAIGRCPKYAEAYFNRATCYIDQKKYREAIADLTAAASILPDRAVIYYNRGYCH
                     EKLKERDAAIADYTTAIQIDPNYFAAYINRGNCYSAEKRYRAAVSDFKDAIAIHPSND
                     TGYYNKGLSEEDLEDFDDARFDLNKAKSLNPGNSNNWIASSRLFLLQGDTVTSFSEIQ
                     KAITMGTDSADAYAIRAMCYRSQQRYADAIADMDHAIRLDSSLSSYYNARGFIYFLAK
                     EQALAIHDYSNAIRLEPDSEEYYFNRGVSYTKLNDHLHARADYLQCLELKPDDYRVYF
                     NMALEDYNQKDYENALTNVMQSIRLDSTICTSYNLRGNIYVQEALGSGDPFVHDSLLH
                     AAVSDGYKVISCEPASPDGWSLRALAYSMMGETKKAMYDINHAISIDSLSADNYSIRG
                     LIEGMSGNTAASIRDCDHALKLDSVNINAYNIRANACRQLSRYDEAIRDYTTLIRLDP
                     GNPSYYYHRGVCWHSKNNEQKAADDIRAALKIDPGNASYKAALQETGK"
     gene            3976..6069
                     /locus_tag="VFU15_01115"
     CDS             3976..6069
                     /locus_tag="VFU15_01115"
                     /inference="COORDINATES: protein motif:HMM:TIGR02226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA and WFA domain-containing protein"
                     /protein_id="HEU4716398.1"
                     /translation="MKFANPVFLFALFSLAIPIIIHLFNFRKFKKVFFTNVRFLKEVK
                     QDTKARNKIRNLLILACRLLAVLFLVFAFAQPYLPRENGTIRTGDKVISIYVDNSFSM
                     EAVGKNGTLLDEAKKDAKEIAAAYKPTDRFQLLTNDFEGRHQRLVNREEFLELLDEVQ
                     PSPAVKNLSEITRRQQDALNTAQGINPGNKQAFIISDFQKTISDFSAIKTDTSIRFHP
                     VPDTAQNRNNIYIDSVWFTSPVRQLNHPEALHIRIKSKSDQDLENVPMRLYVNGEART
                     PSSFSIAPYSVTDTIIYFTLREPGLEQGLIEINDYPVTFDDKFYFSFNVLKSIPVLAI
                     DPSAQAGMQESTDYLQTLFGNDSTFSFAAYEEDKIDYALFPDYRFIVLNSLKTVSSGL
                     ATELRKFVDNGGSLFVFPGPDADLDSYREFLSPLGVNYFEKADTARTVVDQLNYSHPL
                     YQGVFEKQAGNIDLPTVYHHYKISFLSHSTEEELMRLRNGDVFAGSFHSGKGNVYLSS
                     VPLTDSWSNFPRHAIFVPTLMQAALFSQPQNELFYTIGSDDPIDIGDVGVVGENVFHL
                     SDPSKNFDIIPAHSVRDGHTLIDVHRQVKEPGNYFVNLGKDLVTGISFDFNRKESDLT
                     TIDPEKAMTEAGLKNFSMILNANKGLTAALTETDYGIRLWKMCVWLVLLFLLCEALLV
                     RFWKTGTKNLTPQAA"
     gene            6075..>7053
                     /locus_tag="VFU15_01120"
     CDS             6075..>7053
                     /locus_tag="VFU15_01120"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEU4716399.1"
                     /translation="MNILIKNATINDPNSPHNGKTADVLIENGVIASIRKKTDAGKIK
                     TIEGNNLHLSPGWMDLHAHLRDPGYEFKETIVTAAAAAAAGGFTAVLAMPDTLPVTHS
                     KAEVEYILKRSQDLPVDIFPAGALSHGLEGKELAELYDMHLAGAKAFTDGDSPVKHAG
                     LLLRALLYAHNFNCRIHVRCDDQSLSHGGQMNEGVMSTSLGLKGIPAVAEEVMVARNI
                     YLAEYAGTPIHFMAVSTPGSVKLIREAKKKGLPVTAAVHAYNLAWNDSLLEDFDTNYK
                     VSPPLRTEAERKALVAAVNDGTIDCITSGHAPEDAESKVVEFDLAAFGIT"
BASE COUNT         1786 a         1982 c         1755 g         1530 t
ORIGIN      
        1 caaggacgtt gacatcgcca gccagttcac cgatccgttt acgttcgaag gaccgacagc
       61 aaccagcacg gacatggaca tggaaatgcc ggacggcacc ataaaaaaag tgtacatgac
      121 aacgaccgac tgcgatcttt ccgtgcagga aaaacagatc gtcgttccct gcgaggtaaa
      181 ttttacgctg acaccgccgc ccgctccgcc ggtaaaataa aaatcagtct tttacagctg
      241 cagaataggc cgcaagacaa cgcccgcgtg ccgcggcatg atcgatgagc ggtagcgggt
      301 atttttccgt tccgaattct ttcacccatt ttttcacgta ttcaaactcc gggtcgaact
      361 tcttctgctg ttcatgcgga ttgaaaatgc ggaagtaagg cgccgcatcc gttcccgttc
      421 cggcggccca ctgccagttg ccgttattcg ccgcgagatc gaaatcaagg agcttcgaag
      481 cgaaatatgc ctcgccccag cgccagtcga taagaagatg tttcaccagg aaagaagcgg
      541 tcatcatgcg gacgcggtta tgcatgaatc ccgtttcatt cagctcgcgc atgccggcgt
      601 ccacgagcgg gaaacctgtc atcccgtttt tccatcgttc gaactcttct tcgttattcc
      661 gccacgcaat acgatcgtac tccttgcgaa acgaatcttt ttcaacatgc gggaaatgcc
      721 agagcacctg catgaagaat tcacgccaga tcagctcgtt cgtccaggtt tcgctcacgg
      781 ccagcgcgcg acggaaaagc gcgcgaatgc ttaccgttcc gaaacgcagg tgaacgctga
      841 gatgcgatgt tgcccggcgc gccgggaaat cccgaagaga agaatacgtg cgcagcattt
      901 catcattcaa cgggggaacg gaacaatcca tctccgtttc ttcaaatccg aaagaagaaa
      961 gaggtggtag cggaacgaaa tccgtcctta aaaaatttcc tgcgcgtttt tccgaaggaa
     1021 actcctgcat gtctttttcc gcgatgcttc tcctccactt gtttttatac ggcgtgaaaa
     1081 cggtgtaggg cattccgtcg tctttgacga tctcgctttt ttcaaatacg acctggtctt
     1141 tgaacgagaa agcgggaatt cctttttccg ccagcactgc ggcaacatct tcgtcacgcc
     1201 tgcgtgcgta aggctcgtaa tcaccgttcc agaatgcgga agcaacgttg aattttccgg
     1261 gcagctcttt ccatatctca acgggctttc cataaaaaac aaaaagacgg ctgccgttct
     1321 tcgccagctg ctcatttatt ttctgaagat tccggtaaat gaaagtaaca cggcgatctt
     1381 ttttatcgtc aagcttcccg agaatatccg tatcgaaaat aaaaacggga agaaccggaa
     1441 aaccgctgtc cagcgccgcc gacaaaccgc ggttgtcgtt cagcctgaga tcacggcgaa
     1501 accagaatac ggagacgcgt tctttcacgg catggtcaat ggcgctgcct gcgcgttacg
     1561 atggcagaat tgccgctgaa attgaattcc cagccgcttt cgaaaatctt cggaaggaag
     1621 cgcttgtaca gcagcgtgcg ctgcgaagct ttgctttcct cctcgccttc cttcgggatg
     1681 ccggtgaatt cgtagcacat gatcttttcg tgctcggcca tgaacatttt gacgatctgc
     1741 acgatcgtgt tcatcacgcg gaagacctcg ccgcggtttg tcaccacgta ttcctccccg
     1801 tcgaactctt cgttgatgac gccgaaaaca atagttgcgt gcaggatgtt gtcctgcctt
     1861 cttccgaaac ggacctcgta ctgcagtccg ctgtcggtcg tgaagaaata aacgccggcg
     1921 gaagcgatgg ccgggggaat tattttataa atgggcacgg tgcccggcat atacaagtga
     1981 ttggggacta aaaataggga aaaaagcctc tgcgctggca ttcacaacaa ggaggccacg
     2041 gcgcaaagaa tcaccgggta tcgaacattt acaaaggaat gtgccaggcg cccggaacta
     2101 ttttcttttc cgtcccggag gaaagaaaaa gcagcagtgc tgcgaagaag agcaaagacc
     2161 ggtccgggaa aaggaacgca tttgcaatgg caatgaccgg tggcggaaag ataccgggat
     2221 gcggcgcttt atttacattt aaagaattat tcaaccctgt ttgtgcaccg ttcgttatga
     2281 aaaacgtcct cgcgctcgtg ctgctgctct ttcttgccgc accggtttca gcccagaata
     2341 aaaaagcgaa aaaatttctc gccgcaggca atgcggcaag cgctgctaaa aattatgacg
     2401 ccgccatcga caactacacg aaagcgatcg gacgttgccc caaatatgcg gaagcgtatt
     2461 tcaaccgggc cacctgctac atcgaccaga aaaaatacag ggaggccatt gccgacctga
     2521 ctgcagcagc ttccatttta ccggacaggg ctgttattta ctataaccgg ggctactgcc
     2581 acgagaagct gaaagaacgg gacgccgcca ttgccgatta cactactgcc atacaaattg
     2641 atccgaacta tttcgctgct tatatcaacc gcggcaattg ctattcggcg gaaaaacgct
     2701 accgtgccgc tgtttcggat ttcaaggatg cgatcgccat acatccttcc aacgacacag
     2761 gctattacaa caaagggctg tctgaagaag acctcgagga tttcgacgat gcacgtttcg
     2821 acctgaacaa agcaaagtcg ctgaaccccg gcaattccaa caactggatc gccagcagca
     2881 ggctgttcct tctccagggc gacacggtca cttccttttc cgagatacag aaagcgatca
     2941 ccatgggaac ggattcggcg gatgcgtacg cgatacgcgc catgtgctac cgcagccagc
     3001 agcgttacgc cgatgcgatc gcagacatgg accatgccat ccgcctcgat tcctcgcttt
     3061 catcgtacta caatgcgcgc ggcttcattt atttcctcgc gaaggagcag gcgctcgcga
     3121 ttcacgatta cagcaatgcc atccgtctcg aaccggacag cgaagaatat tatttcaacc
     3181 gcggtgtctc ctacacaaag ctgaacgatc atctccatgc cagggcggac tatcttcaat
     3241 gcctggagct gaagcccgac gactatagag tatacttcaa catggcgctg gaggattata
     3301 accagaagga ctatgaaaac gcgttaacga acgtgatgca atccattcgt ctcgacagca
     3361 caatatgcac ttcgtacaat ctccgcggca acatttatgt acaggaagcg ctcggatcag
     3421 gcgatccttt cgtgcacgat tcccttttgc acgctgccgt ttccgatggc tacaaagtca
     3481 tttcatgcga gcccgcatct ccggacggat ggagcctgcg tgcgctcgcc tattccatga
     3541 tgggcgaaac aaaaaaagcg atgtacgaca ttaatcatgc catttccatc gacagcctta
     3601 gtgcagataa ttactcgatc cgcggcctga tcgagggaat gtcgggaaat acggcagctt
     3661 ccatacgcga ctgcgatcac gcgctgaagc tcgattccgt caacatcaat gcgtacaaca
     3721 tacgggcgaa tgcctgccgc cagctcagcc ggtatgatga ggccatcagg gattacacca
     3781 cgctcatccg gctcgatccg ggcaatccat cctattatta tcaccgcggg gtatgctggc
     3841 acagcaagaa caacgagcag aaagcggccg atgatatccg cgctgcgctg aagatcgatc
     3901 ccggcaatgc gtcttacaaa gcggcattgc aggaaacggg gaagtgagaa agcttttgct
     3961 tacctttggc ttttgatgaa atttgccaac ccggttttcc tcttcgcgct tttttcgctg
     4021 gcaatcccga tcatcattca cctttttaat ttccggaaat tcaagaaagt ctttttcacg
     4081 aacgtccgtt tcctgaaaga ggtgaagcag gacacgaagg cgcgcaacaa gatccgcaac
     4141 cttctcattc tcgcctgccg cctgctggct gtactcttcc tcgtcttcgc gttcgcgcaa
     4201 ccttatcttc caagggaaaa cggcaccatc cgcacgggcg acaaagtcat cagcatttac
     4261 gtggataatt cgttcagcat ggaagccgtg ggaaagaacg gcacgctgct cgacgaagcc
     4321 aaaaaggacg cgaaggaaat tgcggcggct tacaaaccta cagaccgttt ccagctgctc
     4381 acgaacgatt tcgaaggaag gcaccagcgg ctggtgaacc gcgaagaatt tctcgagctg
     4441 ctcgacgaag tgcagccgag cccggcggtg aaaaatcttt ccgagatcac gcggcggcag
     4501 caggacgcgc tgaacacggc gcagggaatc aatcccggca acaaacaggc gttcatcatc
     4561 agcgatttcc agaaaaccat ctccgatttt tccgccatca aaaccgacac gtcgatccgt
     4621 ttccatccgg ttccggacac cgcgcagaac cgcaacaaca tttacatcga ttccgtctgg
     4681 ttcacttctc ccgtccgcca gctcaaccac ccggaggcgc ttcacatccg catcaaaagc
     4741 aaatccgacc aggacctgga aaacgttccc atgcgtcttt acgtgaacgg ggaagcgcgt
     4801 acgccttcga gcttcagcat cgcgccttac agcgtcaccg acacgatcat ttatttcacg
     4861 ctgcgcgagc ccgggctgga gcagggactg atcgagatca acgattatcc cgtgacgttc
     4921 gacgataaat tctatttctc gttcaatgtc ctgaaatcaa ttcccgtcct tgcgatcgat
     4981 ccttccgcgc aggccgggat gcaggaatcc accgattacc tgcagacact tttcgggaat
     5041 gattccacct tcagcttcgc tgcttacgaa gaagacaaga tcgattacgc cctgttcccg
     5101 gattaccgct tcatcgtgct gaacagtttg aaaaccgttt catccgggct cgccacggaa
     5161 ctcaggaaat tcgttgacaa cggcggaagt cttttcgttt ttcccggacc cgatgcggat
     5221 cttgacagct accgcgaatt cctttccccg ctcggcgtga attacttcga aaaagcagac
     5281 accgcaagaa cggttgtcga ccagctgaat tacagccacc cgctttacca gggtgttttc
     5341 gaaaaacagg ccggcaacat cgatcttccc acggtttatc atcactacaa aatttcattc
     5401 ttgtcgcaca gcaccgaaga agaattgatg cgcctgcgca acggcgacgt ttttgcagga
     5461 agcttccaca gcggaaaagg gaacgtgtac ctcagttctg ttccgctgac ggacagctgg
     5521 agcaatttcc cgcgccacgc cattttcgtt cccacgctga tgcaggcggc gcttttcagc
     5581 cagccccaga acgagctgtt ctacacgatc ggttccgacg acccgattga catcggcgac
     5641 gtgggcgtgg tcggtgaaaa tgtttttcat ctttccgatc cctcgaaaaa cttcgacatc
     5701 atcccggcac acagcgtacg cgacgggcac acactcatcg acgttcatcg ccaggtgaaa
     5761 gagcccggca attatttcgt caacctcgga aaagatctcg tgacaggaat ttctttcgat
     5821 ttcaaccgga aggaatccga cctgaccacg atagatccgg agaaagcaat gacggaagca
     5881 ggactgaaga atttttccat gatcctgaac gcaaacaaag ggttgactgc agcgctgacg
     5941 gaaaccgatt acggcatacg cctgtggaaa atgtgcgtat ggctggtgct gctgttcctg
     6001 ctttgcgaag cgctcctcgt ccggttctgg aaaaccggta cgaaaaacct gacaccacaa
     6061 gccgcctgaa taaaatgaac atcctgatca aaaacgcaac gatcaacgat ccgaactctc
     6121 cgcacaatgg aaaaacggcg gacgtgctca tcgagaacgg cgtcatcgct tccatccgca
     6181 aaaagaccga tgccgggaaa ataaaaacga tcgaaggaaa taatcttcat ctcagcccgg
     6241 gctggatgga tcttcatgcg cacctgcgcg atcccggcta tgagttcaag gaaacgatcg
     6301 taacagctgc tgccgccgcg gcggccggag gattcaccgc cgtgcttgcg atgcccgata
     6361 cgcttcccgt aacgcattcc aaagcggaag tggaatacat tctcaaacgt tcgcaggatc
     6421 ttcccgtaga catttttcct gcgggcgcgc tgtcgcacgg actcgaagga aaagagctgg
     6481 cagaacttta cgatatgcat ctcgccggtg caaaagcgtt caccgacgga gacagcccgg
     6541 taaaacatgc cgggctcctg ctgcgcgcgc tgctgtacgc gcataatttc aactgccgca
     6601 tccatgtgcg ttgcgacgat cagtcgcttt cccacggagg acaaatgaac gaaggagtga
     6661 tgagcacctc gctcgggctg aaaggaattc ccgcggttgc cgaagaagtg atggtggcac
     6721 ggaacattta ccttgccgaa tacgcgggca cgcccatcca cttcatggcc gtcagcacgc
     6781 cgggaagcgt gaagctcatc cgcgaagcga agaaaaaagg attgccggta accgcggccg
     6841 ttcacgcgta caatctcgcc tggaacgatt cgctgctgga agatttcgat acgaattaca
     6901 aagtaagtcc cccgctgcgg acggaagctg agcgcaaggc gctggtggcg gccgtgaacg
     6961 acggaacgat cgattgcatc acatccggtc acgcgccgga agatgcagaa tcgaaagtcg
     7021 tggagttcga cctggccgca ttcggcatca ccg
//
LOCUS       DASTSF010000040         2682 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1083964,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000040 DASTSF010000000
VERSION     DASTSF010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2682
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1083964"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(161..649)
                     /locus_tag="VFU15_01125"
     CDS             complement(161..649)
                     /locus_tag="VFU15_01125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716400.1"
                     /translation="MITTASITDYNPNKHKLFFLCQYRQIVMLEILGPDDSYKYKYLW
                     SCILRDDNDNTQVAKIWLAKDGTTLWLDHVSSVPSGNGNEQNILFDSAENSFWNDSNG
                     IIVGTGDLRDLSDTQSDLDSFLLLWEIPVEEQDGSVHPIYIENTAVFEINIYSQNSPS
                     KK"
     gene            788..2290
                     /locus_tag="VFU15_01130"
     CDS             788..2290
                     /locus_tag="VFU15_01130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716401.1"
                     /translation="MTAPNTNLESLINALTSQEQRYFRLFAGRHGQPGDSRYLHLFDW
                     LAKGQNGLEKNGGQTSSASERNYLARMILQSLRVYHDGGMPELELRNLVANVLVLQKK
                     RLMNSCMAELKKAKTLAALYEQYTVLLDLFSIEATMLIERQQKNLANDLEELHREMIA
                     VEKQLEMQQTLFRLSNRIFIHSRSAYHAREEDRRDSITKLMHEAQLIAVPAHSLRLQH
                     LQLKIMAVGAVCLGNFEQAFSCYEKLQQLWEAYPGRIEADKSNYRKVLSNFLVVCHAM
                     GDFNSMRRLLDTIRKLKPDNLEDEAEQFQNVFYLELLYLMNTDGFESLDPLVKEIEAG
                     IQKYATKINKAREFAIYYNIGIAYFLSARYRQAVPWLQRIIDNPKTDHRKDIQTAARL
                     VRLILYYELDKFDTLEYELINVERYLRQRKAWYAYEASVLKMVGKLLSAGTPDKNSVV
                     RKLKDQLTETMSGKTSATLPGLSELNCWMHSKLSGQTMRELLRKQDEARA"
BASE COUNT          764 a          628 c          642 g          648 t
ORIGIN      
        1 ttcaggtgcg taaagtcatt gataagacgg cggaattcct tgtaaacagg ggcaaatagg
       61 ttattgcagg aagagttgaa ggataaactg tattgacggg tgtcaaacaa aataaccttg
      121 cttatttttt cggagatatt attggtaagc atataggggc ttattttttc gaaggtgaat
      181 tctgagaata aatattaatc tcgaacaccg ccgtattctc aatataaatc gggtggacgc
      241 taccgtcttg ttcctctacc gggatttccc acagcaataa gaaggagtcc aggtcagatt
      301 gtgtatcgga aaggtcccgc agatcgcccg tgccgactat aattccgtta ctgtcgttcc
      361 agaaactgtt ctccgcactg tcgaacaaaa tgttttgttc attaccattc ccgcttggta
      421 cactgctcac gtgatcgagc cataacgttg taccgtcttt ggccagccag atcttggcca
      481 cctgcgtgtt atcgttgtca tcgcgaagaa tgcacgacca gaggtattta tacttgtacg
      541 aatcatccgg tcccaggatt tcaagcataa cgatctggcg gtattgacac aaaaagaaca
      601 gcttgtgttt gttgggattg tagtccgtta tggatgcagt cgtaatcata tacaggaatt
      661 ttagatttgc gacaaaggaa acctgttgga tttacgcagg caactctgtt tttatttaat
      721 attttaagtc cgatcgtatc caaaacattc gtatatattt gatatccaac tgataaacga
      781 taaagagatg acggccccga ataccaacct cgaatcactt ataaatgcat tgacgtccca
      841 ggagcaacgg tatttccgcc tttttgcggg aagacacggt cagccgggag acagccgtta
      901 cctgcatttg ttcgactggc tggcaaaggg acaaaacgga cttgaaaaaa acggcgggca
      961 aacctcttcc gcttcggaga gaaattacct ggcccggatg atccttcaaa gccttcgcgt
     1021 ttatcatgac ggcggcatgc ccgagctcga gctccggaac ctcgtagcca acgttctcgt
     1081 tttgcaaaag aaacgcctga tgaattcgtg catggctgaa cttaaaaaag caaagacgct
     1141 tgcagcgctc tacgaacaat acactgtgct gcttgatctt ttttcgatcg aagcaactat
     1201 gcttatcgaa aggcagcaaa aaaacctggc gaacgacctc gaagaacttc acagggagat
     1261 gatcgccgtg gagaaacaac ttgaaatgca gcaaacgctg tttcgtttgt ccaaccgtat
     1321 ttttattcat tcccggtcgg cctatcacgc ccgcgaggaa gaccgtcgtg attccatcac
     1381 gaagctgatg cacgaagctc agctgatcgc agttcccgca cattcgctgc gactgcagca
     1441 tcttcagctg aaaataatgg cagtcggggc ggtctgcctc ggtaatttcg aacaagcctt
     1501 cagttgctat gaaaaacttc agcagttgtg ggaagcttat cccgggcgca tcgaagccga
     1561 taaaagcaat taccgaaaag tgctttccaa tttcctggtc gtatgtcacg caatgggcga
     1621 tttcaattcc atgcgccggc tgctcgatac gatcaggaaa ctgaagccgg ataacctgga
     1681 agatgaagcc gagcaattcc agaatgtatt ttacctggaa ctgctttatc tcatgaatac
     1741 cgatggattc gaaagcctgg atccgcttgt gaaagaaatc gaagccggga ttcagaaata
     1801 tgcaaccaag atcaacaagg cgagggaatt tgcgatttat tacaacattg gtatcgcata
     1861 ttttctttct gcgcggtaca ggcaggccgt gccctggctg cagcgcatca ttgataatcc
     1921 caaaacagat catagaaagg acatccagac ggcagcgaga ctggtccggc ttattctgta
     1981 ttatgaactc gacaaattcg acacgctgga atatgaactg atcaacgtgg agcgctacct
     2041 gagacaacgt aaagcatggt atgcgtatga agcttcggtt ctgaaaatgg taggtaaatt
     2101 gctttcagcc ggtacgcccg ataagaatag cgtcgttcgg aaactcaagg atcaattgac
     2161 agaaaccatg tccggcaaaa catccgcgac attgccgggc ctgtcggaac taaattgctg
     2221 gatgcatagc aaactgagcg ggcagacgat gcgcgaatta ctgagaaagc aggacgaggc
     2281 cagagcctga tgcggctgat gaacccggtg cagcaagtta ggttgcgtag aaaaaatttg
     2341 cgaaacaaaa tgtctaatga acattcgccg caaaatcctg aatgagcgac agcgaatcat
     2401 cgattccccg gaataaaaaa aggcatccgg atggatgcct tgtgtaatcg cctttcacga
     2461 tctgccgcgt ccggatcagc gtccgccgac gattcactgt cacccgcgca tagatcggat
     2521 acccggcccg ggattttttc tcagtccgta ggaaaaatcg cacagaaaat gaatcggttc
     2581 tcatgacgtt cggtcttcgg gttatcaaca ggtagcacgc cccgcccaca ggtagcacag
     2641 aggtaaccca aacgtagaaa aacaaagcaa aaacccgaaa cc
//
LOCUS       DASTSF010000041        19239 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_190844,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000041 DASTSF010000000
VERSION     DASTSF010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 19239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19239
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190844"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2273)
                     /locus_tag="VFU15_01135"
     CDS             complement(<1..2273)
                     /locus_tag="VFU15_01135"
                     /inference="COORDINATES: protein motif:HMM:NF024962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprB repeat-containing protein"
                     /protein_id="HEU4716402.1"
                     /translation="MKNSVHCRNSALTVFLLFSLASARLHGQNSTSWTATYDSEKSFI
                     ENKGQFYPRQKFGPDNHVLYGYDGSCTRIFFEKQGVTFNFTQKSKSEEMDGPEKGGKF
                     KSQEEFEREEKKEKAAVLKLDEVTYYWEGANPDAQVIAEEKTGNYFNYSFYVNGVEHN
                     ENNIYGFTKLIYKNLYPNIDVEYTFHPTDGIKYAIIVHPGGDVSKIKMVYSRGVSIGR
                     NGDLHITTSFGDIIDHAPVTFYQDNPSEIISSRFVEKGKSISFGLGAFDGSRTIVIDP
                     WTQTPVLNNSKKVWETETNSSGDVYIYGGDSFIKLLKYNSTGTLQWTYTSPWDSANYW
                     IGGFITHPTTGESYMTSGSNGEIRKISNAGASVWSNNPNGLTSYEYWSLAFNCDLTQL
                     VVAGTRLAFSIPTPVIRGVIMNINLASGAQLSTTVVGYGSTLSIPPNVQEVSSICYAP
                     NGNFYYLTLDTMGCINNALTAVNFKVGTGYAFDYYIPGYGFGTKQPISAIRANATAVY
                     TLNGATVEKRDLNTGAVLASAAIPGGSYTNTFGGRHVNGNGGIDIDNCGNVYVGSVNS
                     VVKYDANLNFISSSPTSFAVYDVDVNSNGEVAACGWSGGTGYIQTLNMSACAQITYTC
                     SLTSPLSVTSSQTNIQCFGQCTGSATVNVSGGTAPYTYAWSPSGGTAATASNLCVGTY
                     TCTITDNASGTTTATFNITQPSAALSSTQSQVNVACSGGCNGSASVVASGGTAGYTYS
                     WSPSGGSAATAGSLCNG"
     gene            2473..3951
                     /locus_tag="VFU15_01140"
     CDS             2473..3951
                     /locus_tag="VFU15_01140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716403.1"
                     /translation="MKPAEDLHQLIQSLSMSEKRFFKIHSSRHVIGGKNNYMRLFDAI
                     ARQDKYDEHEIKKKFHGETFIRHLPSEKHYLYGQVLDALNAYNRDKTFLSRYSNAIIS
                     IEILFNRGLFSHVRKLIKRVRPDAVRLEKFSTLLMLQRWETLVHIQAEDDKNLNRSLQ
                     EELRILDVIRNQTSIMRIAFNIQVQIDKGKATPRFIRETERELRKIFPPPPDIMSFWS
                     KYYFHSGIGLLATVSGKLLLRYTSYKEIKRTMDNSPQFIGELPVIYHINMNNLVNVMF
                     FLEKYDEVESLIGRQRVFLSENHLHIPNLERLVFYNTAESELFLYYKTGRHEKAAVLA
                     HSIGNDIRRAGANAHPSMYDLIFFMAVSMLMIGNYKEATKWLNRILNAEKKINLRKEL
                     LITSRILYLVILYETHDLLFENRLRAARRYVQNEPLFGIHAETLDIFSMLVSFHAGKK
                     NRAELVKRISLLRKEVIRANEKSLNKQFDFAGWMEKKIRFQS"
     gene            complement(3980..4513)
                     /locus_tag="VFU15_01145"
     CDS             complement(3980..4513)
                     /locus_tag="VFU15_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017258793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEU4716404.1"
                     /translation="MSRKIILYIAASVDGYIAKPNDNLDFLSIVQQEGEDYGYGDFIR
                     SVDTVILGRKTYDWVMKQVPVFPHADKTTFVITRTEKPSIGRTNFFTGHLHELITRLK
                     NEEGKNIFIDGGAEVVNALLKENLIDELIISVIPVLLGEGVRLFSDGRPEEKLELVSA
                     KPFRKGLVQLHYRRIDG"
     gene            complement(4922..6838)
                     /locus_tag="VFU15_01150"
     CDS             complement(4922..6838)
                     /locus_tag="VFU15_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEU4716405.1"
                     /translation="MDTILNYWWVGLLILLILLYKLIFRLLGIIIVPEDKIGLVTKKF
                     VLLGNKRQLPPGRIIATEGEAGFQAQTLVPGIYFWKWIWQYEIRLQPLTVIPEGKIGM
                     LVAKDGKELETGCILARRVECNSYQDAGAFLRNGGRKGRQSAILTAGTYRINTYLFDV
                     LVYGMTSISENMVGIVTTLDGKPIDEGSIAGRQIEGHNNFQDADTFLNNGGNKGLQPQ
                     VILAGSYYLNPWFVQVEQVKMTEIPIGHVGVVISFVGNEGKDLSGVEFKHGNIVSKGE
                     KGVWAEPFGPGKYPVNPYIMKVELVPTTNLVLNWASARSESHQLDKNLSTITVRSKDG
                     FPFNLDVSQIIHIPTTEAPKVIARFGNMINLVSQVLEPTIGNYFRNSAQDADVIAFLT
                     SRKERQNSAKEHIGKVLEQYNVFGVDTLIGDISPPESLMKTLTDRKLAEEQNVTFEMQ
                     KKAEITRQALSKETSIADMQREIVQADQGVLIANRIADAAVKKAEGEASSVKIQANAE
                     SERIRVTAGAEAERTKLLANAEAEKVKVLANAEAEKISVTGNAEAEKILAIGKSSAES
                     YKLAVSAMGGDNFTRLKVTEAIGKDRIKIIPDVLIAGGGADSPNGPISGLLGLKLMED
                     LAKKATGNDESKAN"
     gene            complement(7021..7887)
                     /locus_tag="VFU15_01155"
     CDS             complement(7021..7887)
                     /locus_tag="VFU15_01155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716406.1"
                     /translation="MKNPLLFFPLILSLMTSCGSSKFKDQMDSALSKRLDSTSAAEEA
                     ARNAPPVNISFDQLSDKNYDGKKVIVEGYVAAPTSSYVSSTSAQIDFYGRKDQFYAAN
                     NVIVSVPVGTGKNTMYSLPDKYTPEDIKITADDKSEIHAGDHVRITGKLSANGMYSSI
                     DLDKVEKIADTDVDYSTLNAMKLTKATWKDSTMDGKLVYAEGTLEIPMMTMDGNYTWF
                     YLHSKDVSNDLTVNISYGNGANKVEPLSDNYRKSDVKIRDHKGDVVGNHKVRVYGVLH
                     SESIYVENIVQL"
     gene            8473..8892
                     /locus_tag="VFU15_01160"
     CDS             8473..8892
                     /locus_tag="VFU15_01160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716407.1"
                     /translation="MEADHISVTLFLFQRTGRALVGVAGSTMMFAGRKTDQDNSRQEK
                     CADNYRFAFNNLSKNLLNHRGVLKRIQVIGQEKAIFHIPRTNYFQICAYFIDYHHLNF
                     CGNLFPGLWRLVSGKKNPFFYGKGNANSLLIGPGFGK"
     gene            8959..9537
                     /locus_tag="VFU15_01165"
     CDS             8959..9537
                     /locus_tag="VFU15_01165"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU4716408.1"
                     /translation="MRIRFLWPKKETQLSDQELIEEYRRTSNSWYAGELFRRYTRLIT
                     AVAYNYLQNNVDTEDAVMEVFEIILRDLQHHEVNNFKTWVYSVTKHLCLKKLRKDAME
                     VTDVEHALRNMTAEEAPKSEALRLEEQLEKLREALLRLSEEQKKCIELFYFKEKSYKE
                     VSELTGFSVNEVKSYLQNGKRNLKGYLVSTEE"
     gene            9594..10643
                     /locus_tag="VFU15_01170"
     CDS             9594..10643
                     /locus_tag="VFU15_01170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEU4716409.1"
                     /translation="MRLYLDGKLNKKSMHEVEKHLIECSLCSAAVDGLTPRRMAEINK
                     LSDHIHRRLSVYMNTPPRVPFFRRFGLALIAGFLFLSGGTAIYLYEHSKNSTPVSKDS
                     VSTYSHPAFIAPPGGSDAASLADNSLSAQQVVSSSSGEEPGPNTGSAGNTDDKASSSA
                     ATDAGQANVTHTVTPGNAGTGAPSSPPGDNNFHQETPPPADGANSRIASSATPVRVLN
                     VIVYPPVTHSDKKGGGQQSSNDGQLKRSSGSNADFKLDEMPTYPGGDEALREYIMQNF
                     NPVVCDKSKLARLSTGVMFTVNSKTGEISAPELSFSISPQIDAELLRVINSMPLWNPG
                     KKRGEVDVMVGITLG"
     gene            complement(10798..11646)
                     /gene="panC"
                     /locus_tag="VFU15_01175"
     CDS             complement(10798..11646)
                     /gene="panC"
                     /locus_tag="VFU15_01175"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEU4716410.1"
                     /translation="MITITTRGELSALLDTQRKSGKKIGFVPTMGALHEGHASLVAEA
                     RRRADTVVVSIFVNPTQFNDAADLKNYPRTPGQDAVLLEKSGADILFLPSVHEMYPNG
                     TNEEVPVPDIGILGKVMEAAHRPGHFEGVINIVSRLFTIVGECTAFFGEKDFQQLAVI
                     RKMVREMNMNVVIVGCPIVRESDGLAMSSRNARLGREERAAAPFISQVLFRSREMWNE
                     KKTAEEIKRFVKEEFEKHSLFRLEYFEIADAGTLEAAVSREGKKVACIAAWLGSVRLI
                     DNVVYA"
     gene            11770..12600
                     /locus_tag="VFU15_01180"
     CDS             11770..12600
                     /locus_tag="VFU15_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HEU4716411.1"
                     /translation="MKKARVLFVSQEITPYLDETPMGYIARYLPQGIQEKGKEIRTFM
                     PRYGCINERRNQLHEVIRLSGMNLIINDTDHPLIIKVASIQAARMQVYFIDNEEYFQR
                     KMVLTDKKGAYFEDNDERMIFFARGVIETVKKLGWAPDIVHCHGWFTGLMPLYLKKAF
                     RDNPLFAETRVIYSVYEEEFPNQLNKNFAKKILIDGIEKSDVEQVAKLPNFVNLNKLA
                     IDYSDAVIKGSEKINPDVEKYIKKSGKNSLDFKSREEYIAAYSEFYDAVLAEEPLLVD
                     "
     gene            12689..13957
                     /locus_tag="VFU15_01185"
     CDS             12689..13957
                     /locus_tag="VFU15_01185"
                     /inference="COORDINATES: protein motif:HMM:NF025458.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4270 family protein"
                     /protein_id="HEU4716412.1"
                     /translation="MASCKKDTDIGANLQPQNELINLLTTDTTSLLTYTVPEDSVRSD
                     KSPTVQLGCLNDGNFGKTTASLFSQFVIPNNLTDINFGTGAAVDSLVLYMRYDFDYYG
                     DTMTTQTFNVYQMQDSISWDSAYYSNQRKSCFPGSIGSASFLPTPRSKSVVEGDTVSG
                     VLRIPISNAWAQNFINWAAGGNMHSNSTLLQVFYGLWIVPDSNLASGGALLRFTPQDT
                     LTGLKMYYHNSTDTASFTFAINAGTAYYSYFQHNFNGISSSSPLYNQLHNPGANSDPE
                     VYIQSAAGLKTKIEFPFLSDWMSSTAPIAVNKAELIIKADPTQASNDLPINKQLYLVY
                     LDSAGNSYVLPDMFENSTYYGGSVITTTSEYHINLARYFQEIVTGRTGNYGMYLKEIS
                     PVIEGRRAVLGSPNSTGYKMYLHLVYTRVN"
     gene            13973..15811
                     /gene="glmS"
                     /locus_tag="VFU15_01190"
     CDS             13973..15811
                     /gene="glmS"
                     /locus_tag="VFU15_01190"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010611557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEU4716413.1"
                     /translation="MCGIVAYIGTRKAYPILVKGLHRLEYRGYDSAGIALIHESLNVY
                     KCKGKVEDLERFASGHDTEGTVGIGHTRWATHGEPNDINAHPHFSGSKKLVIIHNGII
                     ENYAPLKAELIKRGHKFESDTDTEVLAHLIEDIQLREKTDLFEAVRIALNGVVGAYAI
                     VILSKENPDQLIAARKSSPLVIGIGNDEYFVASDATPIVEYTKNVVYLEDEQIAMVKR
                     GEDLLIRTIKNKKITPYIHELQIQLDAIEKGGYDHFMLKEIYEQPRSIKDSMRGRLNP
                     TEGVVALGGIRDYLQKFTTAKRVIIIGCGTSWHAGLVAEYLFEELARIPVEVEYASEF
                     RYRNPVIYEDDIVIAISQSGETADTLAAIELAKSKGATIFGVCNVVGSSIARATHAGS
                     YTHAGPEIGVASTKAFTAQVTVLTLMALLLGHKRGMISERHYHELLSELEAIPNKVEK
                     LLTTNDKVKYLASVYKDARNFLYLGRGYSFPVALEGALKLKEISYIHAEGYPAAEMKH
                     GPIALIDDEMPVVVIATKGASYEKVVSNIQEVKARKGKIIAVVTEGDETVRKMADHVI
                     EIPETDEKLVPLLAVIPLQLLAYHIAVMRKCNVDQPRNLAKSVTVE"
     gene            complement(15841..16245)
                     /locus_tag="VFU15_01195"
     CDS             complement(15841..16245)
                     /locus_tag="VFU15_01195"
                     /inference="COORDINATES: protein motif:HMM:NF017007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HEU4716414.1"
                     /translation="MQFRFLLVALLFCSAVFANEKQPVRVPCIILHDTLVIDTSAIGV
                     SRDTMQVKATAKRAANDRFIAAVLAFPVPFGILGLHRIYLGTAPYVPVVYIVTAGGFG
                     LLPLLDFIAIVSASDEEFESFHGNPNVFMWVK"
     gene            complement(16313..16948)
                     /locus_tag="VFU15_01200"
     CDS             complement(16313..16948)
                     /locus_tag="VFU15_01200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716415.1"
                     /translation="MKKSIFSAALLFAFVFAGLPKTMQAQKYQGQVVIGANCAFSLVG
                     LVMNTAFNVADRGIVGLSTSVTPGLSGTVDVGVTDRFSLGVSHFYQSANAHWSTYTDN
                     NGITHTGDFHYRITRQNTALRALFHFGDNDDIDPYFGIRLGYSYWSSSSNIADVDQTF
                     DLSKFKPHLWPQALFGVRYFFIPNLGVNAEIAAGPPYYLSVGLNARFGGTK"
     gene            complement(16969..17574)
                     /locus_tag="VFU15_01205"
     CDS             complement(16969..17574)
                     /locus_tag="VFU15_01205"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015810019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEU4716416.1"
                     /translation="MAFELPKLAYPYNALEPNIDARTMEIHHTKHHQAYVTNLNNAIA
                     GTDAEKMSIEDICRNISKYAVAVRNNGGGHYNHSLFWQVMKPNGGGAPSGDLASAINA
                     AFGDFEKFKTQFSSAAATRFGSGWAWLIVGADKKLAICSTPNQDNPLMDIADVKGTPI
                     LGLDVWEHAYYLNYQNRRPDYISAFWNVINWEEVARRLQTA"
     gene            17703..17936
                     /locus_tag="VFU15_01210"
     CDS             17703..17936
                     /locus_tag="VFU15_01210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716417.1"
                     /translation="MKKVFAIAFAGAVIAGFASCGSSDADKKADSANAVQTKKEMDET
                     GDHMIDSMNRANAVMDSTNKAGAKTDTAKSAGK"
     gene            complement(17996..>19239)
                     /locus_tag="VFU15_01215"
     CDS             complement(17996..>19239)
                     /locus_tag="VFU15_01215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEU4716418.1"
                     /translation="VMAVVPGGPSEHSGLLPGDRIFKANDTVIAGKNISDKKIRTMLR
                     GPEGTKVRISVRRPFPAKDFVVEITRGSVPIYSVEAKYMLDSETGYIRLSRFAETSYR
                     EFTEAADSLLAKGMKKMVLDLRGNGGGYMDAATDIADEFLPEGKLIVYTKGRTDGEHR
                     VVATRKGKLEKMPLAILVDENSASASEILAGAIQDNDRGVIIGRRTFGKGLVQEEKHL
                     TDGSGFRLTIARYYTPTGRCIQKPYDKGLENYESDEEHRYKHGELLNKDSIKFPDSLK
                     YKTPGGKTVYGGGGIMPDIFIPLDTAGGSDYLSALFGHDIFSLWAMNFASHNRDALVK
                     NGREDFRRNFSVSDEMVNGLELIAAQNGIKKNDAQEKRSDALIRRYLKAAVARSIWGD
                     EGYYLCWNDEDAAIAAAMKSL"
BASE COUNT         4683 a         5104 c         4788 g         4664 t
ORIGIN      
        1 ttcccgttgc agagactgcc cgctgtggcc gcgctgccgc cggacggcga ccaggaatag
       61 gtgtatcccg ctgttccgcc cgaagcgacg accgaagccg atccgttgca tccgccgctg
      121 cacgccacgt tcacctgcga ctgcgtggag gaaagcgctg cggaaggttg cgtgatgttg
      181 aatgtggccg tcgtggtgcc gctcgcgtta tcggtgatcg tgcaggtgta tgttccgacg
      241 cagaggttgc tggccgtagc cgccgttccg cccgaaggcg accatgcata cgtgtaaggt
      301 gcggttccgc cgctgacgtt cactgttgca gaacctgtgc actggccaaa gcactggatg
      361 ttcgtctggc tggaagtgac ggaaagtggt gaggtcagtg aacaagtgta cgtgatctgt
      421 gcacaggccg acatgttcag cgtctggatg tagcctgtgc cgccgctcca gccgcatgcg
      481 gcgacttcgc cgttggaatt cacatcgaca tcatacacgg cgaaggaagt cggtgaagaa
      541 gaaatgaaat tcaggttcgc atcgtacttg acaacgctgt tcaccgatcc gacgtaaacg
      601 ttcccgcaat tatcaatgtc gatcccgccg tttccgttca cgtgccgccc gccgaatgtg
      661 ttcgtatacg atccgcccgg gatagccgca gaggcgagga cggctcccgt gttgaggtcg
      721 cgtttttcca cggtggcgcc gttcagcgtg tacacggccg ttgcattggc gcgtatggcg
      781 gaaataggtt gcttcgttcc gaaaccgtaa cccgggatat aataatcgaa cgcatagccg
      841 gttcccactt tgaaattcac cgcggtaagc gcattgttga tgcagcccat cgtatcgagc
      901 gtcaggtaat agaaatttcc gttcggcgca tagcagatgg agcttacctc ctgcacgttc
      961 ggtggaatgg ataacgtcga tccgtagccg actacggttg tgcttagctg cgcaccgctg
     1021 gcaagattga tgttcatgat caccccgcgg atcaccggtg tgggaatgct gaacgcaaga
     1081 cgcgttccgg ctaccaccag ctgcgtgaga tcgcagttga acgccaggct ccagtattcg
     1141 tagctcgtta gtccgttcgg gttgttgctc cacacggaag cgcctgcatt gctgatcttg
     1201 cggatctctc cgttcgatcc ggacgtcata tacgattcgc ccgtcgtcgg gtgcgtgatg
     1261 aatccgccga tccagtaatt cgccgaatcc cacggcgagg tgtaggtcca ctggagcgta
     1321 cccgttgaat tatatttcag cagcttgatg aatgaatcgc cgccgtagat ataaacgtcg
     1381 cccgaagaat tggtttccgt ttcccacact ttcttcgaat tgttcagcac cggcgtctgc
     1441 gtccagggat cgatgacgat cgtgcgtgat ccgtcgaatg caccgagacc gaacgaaatg
     1501 cttttccctt tttcaacaaa gcgcgatgaa atgatctccg aaggattgtc ctggtaaaaa
     1561 gtgacgggcg cgtgatcgat gatatcgccg aaagaagttg tgatgtggag gtcgccgttc
     1621 ctgccgatgg aaacgccgcg ggaataaacc atttttattt tcgaaacatc tccgcccggg
     1681 tgaacgatga tcgcatactt gatcccgtcc gtgggatgga acgtgtattc gacgtcgatg
     1741 ttcgggtaaa ggtttttata gataagcttt gtgaacccgt aaatgttgtt ctcgttatgc
     1801 tcgacgccat tcacatagaa tgaatagttg aaataatttc ccgttttttc ttcggcgatc
     1861 acctgcgcat ccgggttcgc tccttcccag taataggtta cttcatcgag cttcagcaca
     1921 gccgccttct ctttcttttc ttcccgctcg aactcttcct ggcttttgaa ttttccgcct
     1981 ttttccggac cgtccatttc ttcgctcttg cttttctgcg tgaagttgaa cgttacgccc
     2041 tgcttttcga aaaagatgcg cgtgcaggaa ccgtcatacc cgtaaagcac gtgattgtcc
     2101 gggccgaatt tctgccgcgg gtaaaactgt cctttgttct cgatgaatga tttttccgaa
     2161 tcatacgtcg ctgtccagga tgtgctgttc tgcccatgaa gtcttgcact tgcaagggag
     2221 aaaagaagga aaacggtgag cgctgaattc ctgcagtgga cggaattttt catgggtgtg
     2281 gcgttgtttt ttgaattctt tgacgaatgc agcaagaaag aatacagggc caaaattaga
     2341 ccgaaaggca ccgggcgcaa gttaaaaagg ggaatattgt tggaagtctg ttcgcataaa
     2401 accggctgaa tcatttctgt tatttataat atttaaccta attttcctga aaatattgga
     2461 agtctgattc gcatgaaacc agccgaagat cttcaccagc tcatccagag cctcagcatg
     2521 tcggagaaac gtttcttcaa gatccactcc tcccgtcatg tcatcggcgg aaagaacaat
     2581 tacatgcgcc tgttcgacgc tattgcaagg caggataaat acgacgagca cgagatcaaa
     2641 aaaaaatttc acggcgaaac gttcatccgc catcttcctt ctgaaaaaca ttacctctac
     2701 ggacaggtgc tcgacgcgct gaacgcctac aaccgcgaca aaacattcct ctcgcgctac
     2761 tcgaacgcga tcatctctat cgagatcctt ttcaaccgcg gacttttcag ccacgtgcgc
     2821 aagctcatca aacgcgtcag gccggatgcc gtgcgcctcg aaaaattttc gaccctgctt
     2881 atgctccagc gctgggaaac gctcgtgcac atacaggcgg aggacgacaa aaacctcaac
     2941 cgcagcctgc aggaagaact ccgcatcctc gacgtgatcc gcaaccagac gtccatcatg
     3001 cgcatcgcgt tcaacatcca ggtgcagatc gataaaggaa aagccactcc gcgcttcatc
     3061 cgtgaaacgg aacgcgagct ccggaaaatt ttcccgcccc cgccggatat catgagcttc
     3121 tggtcgaaat attatttcca ttcgggcatc ggcctgctcg caacggtttc cggcaagctc
     3181 ctgctgcgct atacctctta caaggagatc aagcgcacca tggacaactc gccgcaattc
     3241 atcggcgagc tccctgtcat ctaccacatc aacatgaaca acctggtgaa tgtgatgttc
     3301 ttcctcgaga aatacgacga ggtggaatcg ctcatcgggc ggcagcgcgt tttcctttcc
     3361 gaaaaccacc tgcacattcc caatctcgaa cgtctcgttt tttacaacac cgccgaaagc
     3421 gaactcttcc tgtattataa aaccgggcgc cacgaaaaag cagccgtcct cgcgcacagc
     3481 atcgggaacg atatccgccg cgccggcgca aatgcgcacc cgtcgatgta cgacctcatt
     3541 ttctttatgg cggtttccat gctgatgatc gggaattaca aggaagccac caaatggctg
     3601 aaccgcattc tcaatgcgga aaaaaagatc aacctgcgga aagagctgct catcacgtca
     3661 cgcattcttt acctcgtcat cctctacgaa acacacgacc tgcttttcga gaacaggctc
     3721 cgcgcggcgc gcaggtatgt gcagaatgaa ccgctgttcg gcatacatgc ggaaacgctc
     3781 gatatatttt ccatgctcgt ttcctttcac gccggaaaga aaaacagggc cgagctcgtg
     3841 aaaagaattt ccttgctgcg gaaagaagtg atcagagcga acgaaaaatc gctcaacaag
     3901 cagtttgatt tcgccgggtg gatggagaaa aagatccggt tccagtcgta atgctcatat
     3961 cgtcacccgg cggcaacgct catccgtcga tccgcctgta atgcagctgc accagtcctt
     4021 tcctgaacgg ctttgccgaa acaagctcca gcttctcttc cgggcgtccg tcgctgaaaa
     4081 gtctcactcc ttctccaagc aaaaccggga taacggaaat gatgagctcg tcaatgagat
     4141 tttctttcag gagcgcattc accacttccg ctcctccgtc gatgaaaata ttttttcctt
     4201 cttcgttttt cagccgcgtg atcagttcgt gcaggtgccc ggtgaagaaa ttcgttctgc
     4261 caatggacgg tttttccgtc cgcgtaatga cgaacgtggt tttgtctgcg tgcggaaaca
     4321 cgggaacctg cttcatcacc cagtcgtatg ttttcctgcc gagaatgacc gtatccaccg
     4381 acctgatgaa atcgccataa ccataatctt ctccttcctg ctgcacgatg gacaaaaagt
     4441 cgaggttatc attgggtttt gcaatgtaac cgtcaacgct cgcggcgata tacaatatga
     4501 tttttctgct cataaaccgt gcgcaattta aatagtttgc ctgcctggaa acaactgcgg
     4561 tcctgcattc gcggaccggc aacaacatgt gatttagtcc tgttctccgg ctggtcagcc
     4621 agggaaactg aacgaggaaa attccatctc tgctgttttt tccgggaatt tcacatttct
     4681 gtaaaagtca gggttgtaat tcctttgtat gaacttaaag ttcgggttac cggggacaga
     4741 atgcaattga tcagggcaat gcgtattctt ttcgcacata ttctgccgtt aatcagaagt
     4801 ttcatcattt cagctcaact ttcacttccg gaaaatcata ctacagggtg atgaatgagt
     4861 tgtttattgc acaaaaaaac agcgcaaagt ttcccctgcg ctgttcccgt tttttgtatc
     4921 atcagtttgc cttcgactcg tcgttcccgg tcgctttttt ggcgaggtct tccatcagct
     4981 tcaacccgag cagcccgctg atggggccgt tgggggaatc tgcgccgcca cctgcgatga
     5041 gcacatcggg aatgatcttg atgcggtctt tgccgatggc ttcggtcact ttcagccgtg
     5101 tgaaattgtc gccgcccatc gcacttaccg caagcttgta cgattcggcg gatgacttac
     5161 caatggcgag aattttttct gcttccgcat tgccggtaac ggaaattttt tccgcttcgg
     5221 cgtttgcgag cactttcact ttttctgctt ccgcatttgc cagcagcttc gtccgttccg
     5281 cttcagcgcc cgccgtcaca cggatgcgtt cggattcggc gttcgcctgg atcttcacgc
     5341 tcgacgcttc cccttccgct tttttcacgg cagcatccgc aatgcggttc gcaatgagaa
     5401 cgccctggtc tgcctgcacg atctcgcgct gcatatccgc gatcgacgtt tccttcgaaa
     5461 gtgcctggcg cgtgatctcg gcttttttct gcatctcgaa cgtcacgttc tgctcttccg
     5521 ccagcttacg gtcggtgagc gttttcatga gcgattccgg cggactgata tccccgatca
     5581 gcgtatcgac gccgaacacg ttgtattgct ccagcacttt gccgatgtgc tctttcgcgg
     5641 aattctgtct ttccttccgc gaagtaagga acgcgatcac atctgcatcc tgcgcggagt
     5701 tgcggaaata gttaccgatc gtcggttcca gcacctggct cacgaggttg atcatgttcc
     5761 cgaagcgcgc aatgaccttc ggcgcttccg tcgtgggaat gtgaatgatc tgcgacacgt
     5821 cgaggttgaa cgggaatccg tccttcgaac gcacggtgat ggtggaaaga tttttatcca
     5881 gctggtgcga ttcggatcgt gcgctcgccc agttgagcac gaggttcgtc gtgggcacga
     5941 gctccacttt catgatgtac gggttcaccg ggtattttcc cggaccgaac ggttccgccc
     6001 acacgccttt ttcacctttg ctgacgatat tgccatgctt gaattccacg ccgctgaggt
     6061 cttttccttc gttgcccacg aatgaaatga caacgccgac atgaccgatc ggaatttccg
     6121 tcatcttcac ctgctccacc tgcacgaacc agggattcag gtaatacgag cccgcaagga
     6181 tcacctgcgg ctgcagacct ttattgccgc cgttgttcag gaatgtatcc gcatcctgga
     6241 aattgttgtg tccctcgatc tggcgtccgg caatgcttcc ttcgtcgatc ggtttcccgt
     6301 cgagcgtcgt tacaatgccc accatgtttt ccgagatcga cgtcatcccg taaacgagca
     6361 catcgaacag gtatgtattg atccggtacg ttccggcggt gagaatggcg gattgtcttc
     6421 ccttgcggcc tccgttgcgc aggaatgccc ctgcatcctg gtaggaatta cattctacgc
     6481 ggcgcgcaag aatgcaacct gtttcaagct ctttcccgtc tttcgccacc agcatcccga
     6541 tctttccttc gggaataacc gtaagcggct gcagcctgat ctcgtactgc cagatccatt
     6601 tccagaaata aattccggga acgagcgtct gtgcctggaa tcccgcttct ccttccgttg
     6661 caatgatgcg gcccggcgga agctggcgtt tgtttccgag cagcacgaat tttttcgtta
     6721 caagaccgat cttgtcttcc gggacgatga taattccgag aagacggaaa atcagcttgt
     6781 acagcaggat gagcaggatc aataatccca cccaccagta attgagaatt gtgtccataa
     6841 tcagggctat tgagatttga agaacgaaat taccggaaac gaaaaccggt ggacatcaac
     6901 ccgcggggaa atagttataa accgccggcc gggttatgaa cagccggaag caaaaatgca
     6961 gcggaaaaat ttccgctgca tcgtgtttgt tttaaattat gccgttgctg aatttcctct
     7021 tcagagctga acgatgttct ccacgtaaat gctttcactg tgcagaacgc catatacacg
     7081 aactttgtga tttcccacta cgtccccttt gtgatcacgg atcttcacat cgcttttccg
     7141 gtaattgtcg gagagcggct ccaccttgtt cgctccattt ccgtatgaaa tgttcacggt
     7201 aagatcgttc gaaacgtcct tcgaatgaag atagaaccag gtatagtttc cgtccatcgt
     7261 catcatggga atttcaagcg ttccttcggc ataaacaagc ttgccgtcca tcgtggaatc
     7321 cttccaggtc gctttcgtaa gcttcattgc gttcagcgtg gaataatcca cgtccgtgtc
     7381 agcgatcttc tcgaccttgt caagatcgat ggagctgtac attccgttcg cgctgagctt
     7441 ccctgtgata cgcacatgat cgcctgcgtg gatctcgctt ttatcgtctg cggtgatctt
     7501 aatatcctcc ggcgtgtact tatccggcaa cgagtacatt gtgttttttc ccgttccgac
     7561 cggtacgctt acgatcacgt tattggcggc atagaactgg tcttttctcc cgtaaaaatc
     7621 gatctgcgcc gatgtgctgg aaacgtagga tgatgtgggc gccgctacgt agccctccac
     7681 gatcactttt ttcccgtcat agtttttgtc ggaaagctga tcaaaagaaa tgttcaccgg
     7741 cggagcgttt cgcgccgctt cttccgctgc gctggtgctg tcgagacgtt tggacagcgc
     7801 gctgtccatc tggtctttga atttcgagct gccgcaggaa gtcatcagcg acaggataag
     7861 agggaaaaag aggagagggt ttttcattta tttttttgac aaagctaaaa aatccggaaa
     7921 gtgcgaccgg gaaacaaaag gtatgaaaac gaaaaatccc ccgtgtggag gatctttcgc
     7981 ggcaaacaca tgaaacacat gtataaagtg aaaaacggct atctgaataa cacgcttttc
     8041 aggagtgcgg attaaaaatg ttttgaatat ccgtgagaac tgtgcatttt atgcgtacgc
     8101 acgttgctct tcttggaacc ctggtaagcg ccgcagtccg gcggtgcttt acaggaaggc
     8161 atgctggtca gcacaagtcc tgccatcaat aatacagttg caaaaattcc ttttctgctg
     8221 taacgggtgc tgaacgattt tgccatgtcg gttttgtttg attttgatat taggtcgccg
     8281 atcattcttt ttccataaaa cttttgcaaa aaagtttttt cgcgagagat gaaacggctg
     8341 aaaacaaaac atcccgacgt tagtcaggat gatttgtggc aaacacatga aacacatgta
     8401 tagaggaaga acggctttcg ggaggaaaac tcgcccttca ggagtacgga tctaagcttt
     8461 attgatcacc tcatggaggc ggatcacatc agcgtgacgc tttttctttt tcagagaacg
     8521 ggtcgagccc tggtaggcgt cgcaggtagc accatgatgt ttgcaggaag gaagactgac
     8581 caggataatt cccgccagga aaagtgtgcg gacaattacc gttttgcttt caacaatctt
     8641 tccaagaacc tgcttaatca taggggcgta ttaaagcgca ttcaggtaat agggcaggaa
     8701 aaggcaatat tccacatccc ccgcacaaac tattttcaga tttgtgcgta ttttattgat
     8761 tatcatcatc ttaatttttg tggaaatctg tttccgggtc tgtggagatt ggtttccggt
     8821 aaaaagaacc ctttttttta tggaaaaggg aacgccaatt cccttttaat cggaccagga
     8881 tttggaaaat gaaaaaaagt gtgtacatta ggattggcaa aagcaaacac atttattcac
     8941 atgtaaatcc taattacggt gcgcattcgt tttctctggc ccaaaaagga aacccagctg
     9001 agcgaccagg aattgattga ggagtacagg cgtacttcca actcttggta tgcaggtgag
     9061 ctgtttcgcc gctatacccg gctgatcact gcagtggctt ataattacct gcagaacaac
     9121 gtggataccg aagacgccgt catggaagtc ttcgagatca tcctccgcga tctgcagcac
     9181 cacgaggtga acaattttaa aacatgggtg tatagtgtta cgaaacacct ctgcctgaaa
     9241 aaactgagga aagacgcgat ggaggtcacg gatgtggagc atgcgctccg caacatgacg
     9301 gcagaagaag ctccgaagtc agaagcgctg aggctggaag aacagctcga aaagctcagg
     9361 gaggcattac tccgcctttc cgaagagcag aaaaaatgca tcgaactgtt ctactttaaa
     9421 gagaagagct acaaagaggt atcagaactt accggtttct ccgtgaacga ggttaaaagc
     9481 tatctgcaga acggaaaaag aaacctgaaa ggctacctgg tatccaccga agaatgacgc
     9541 ctaaagagct gaaacgaatt ttccaaaaag gagagtgcct ttcccttgat accatgcggc
     9601 tttaccttga cggtaagctc aacaaaaaaa gcatgcatga agtggagaag cacctcatcg
     9661 aatgcagtct ctgctccgct gccgtggatg gacttacccc gaggagaatg gcggagatca
     9721 ataagctttc cgaccacatc caccgccggc tttccgttta tatgaacacg cctccgcgtg
     9781 ttccgttctt ccgtcgtttc gggctcgccc tgatagccgg ctttcttttt ctttccgggg
     9841 gaaccgccat ttatttatat gaacacagca aaaattcaac cccggtaagc aaagattccg
     9901 tttcaacgta tagccacccg gcttttattg ctccaccggg gggatccgat gccgcttcac
     9961 tggcagacaa cagcctatcc gctcaacagg tcgtttcttc ttcttccggc gaagaacccg
    10021 gacccaacac cggatctgcc gggaacactg acgataaagc cagctcttcc gctgcaacgg
    10081 atgcaggaca ggccaacgta acacataccg tcaccccggg caatgccgga accggggcac
    10141 catcatctcc tccgggcgat aataattttc accaggaaac acctccgcct gccgacggcg
    10201 ccaattcacg catcgcctct tccgcaacgc ccgtacgcgt gctgaacgtg atcgtttacc
    10261 cgccggtgac gcacagcgat aaaaaaggcg gcgggcagca atcctcgaac gacgggcagc
    10321 tcaagcgcag ctcgggcagc aacgccgatt tcaaactgga tgaaatgccc acctatcccg
    10381 gcggagacga agcgctgcgt gagtacatca tgcagaactt caatcctgtg gtgtgcgaca
    10441 aatcaaaact ggcccggctg tcgacgggag ttatgttcac ggttaattcc aaaacaggcg
    10501 agatcagcgc gcccgaattg tctttcagca tttccccgca gatcgacgct gaactgctgc
    10561 gcgtgatcaa ttccatgccg ctgtggaatc ccggaaaaaa acggggcgaa gtggatgtaa
    10621 tggtcggtat tacgctgggc taatttcttc ggggtttttc cttccctgta tttcaatttc
    10681 tttcagaaaa cagctgccgt gcgctgcgct tcccgccttc tttaaaaccg aacgaccgga
    10741 ttaatcctcc gcaataattt tttcctgccg aacttctctc cgttcgccgg aaaaagatca
    10801 ggcataaaca acattgtcga tcaggcggac ggaacccagc caggcggcaa tgcaggccac
    10861 cttttttcct tcacgcgata cagctgcttc aagtgtgccg gcgtcagcga tctcgaaata
    10921 ctcaaggcgg aaaagagaat gtttttcaaa ttcctctttt acaaaccgct ttatttcttc
    10981 cgctgttttt ttttcattcc acatttcacg ggaacgaaaa agcacctgtg aaatgaacgg
    11041 ggccgcggcg cgttcttccc tgccgagccg tgcattacgc gaagacatgg caagtccgtc
    11101 gctttcgcgc acgatcgggc agccgacgat cactacgttc atgttcatct cgcgcaccat
    11161 tttgcggatc acagcgagct gctggaaatc cttttccccg aaaaatgccg tgcattcgcc
    11221 cacaatagtg aacagccggc tgacgatgtt gatcacacct tcgaaatgac ccgggcgatg
    11281 cgcagcttcc atgaccttgc cgagaatgcc gatatcggga acgggaactt cttcatttgt
    11341 tccgttcgga tacatttcat ggacggacgg caggaacagt atatcggcgc ccgacttttc
    11401 cagcagaaca gcatcctgcc ccggcgtgcg cggataattt ttcagatctg cggcatcatt
    11461 gaactgggtc ggattgacga aaatgctgac caccactgta tccgcccggc gccgcgcttc
    11521 cgcaaccagt gaagcatgac cttcgtgcaa tgcgcccatg gtgggtacga acccgatctt
    11581 ttttccggat tttctttgcg tatcgagcag cgcggataat tctccgcggg tggtaatcgt
    11641 tatcaaaaag aagtgttatt taatcccaaa agcgggtgag aaaagggggt gaaactacgg
    11701 tttttctcca tttcagcctt tttttttatt atttttgtat tccttttcac aaacagctcc
    11761 cacctacaaa tgaaaaaagc aagagttctc ttcgtctcac aggaaattac cccttatctc
    11821 gacgaaacac cgatgggcta tattgcccgt tacctgccac agggcatcca ggaaaaagga
    11881 aaagaaatcc gcacgttcat gccccgttac ggctgcatca acgagcgccg gaaccagctg
    11941 catgaagtga tccgtctttc aggtatgaac ctgattatca acgatacaga tcatccgctg
    12001 atcattaaag tcgcttctat ccaggcggca cggatgcagg tttatttcat cgataacgag
    12061 gaatatttcc agcgcaagat ggtgctgacg gacaagaagg gcgcctattt cgaggataac
    12121 gacgagcgca tgatcttctt tgcccgcggc gtgatcgaaa cggttaaaaa gctcggatgg
    12181 gctccggata tcgtgcattg ccatggctgg tttaccggtc ttatgccgct ttatcttaaa
    12241 aaagcattcc gggataaccc actttttgcc gaaacacgcg tcatatactc tgtatatgaa
    12301 gaagaattcc cgaaccagct caataaaaac ttcgccaaga agatccttat tgacggcatt
    12361 gaaaaatcag acgtcgaaca ggtggccaag cttccgaact tcgtcaatct caacaaactg
    12421 gctatcgatt attcggacgc cgttatcaaa ggttccgaaa agatcaaccc ggacgttgaa
    12481 aaatacatca agaaaagcgg caagaattcg ctcgatttca aatcgcgtga agaatatatc
    12541 gcggcttatt ccgaatttta cgacgcagtt ctcgccgaag agccgctgct tgtcgactga
    12601 ctttatgaat ctgaacatac tgcgtgaaaa cgtgaaaatt ttgtcccgta attccgggac
    12661 gaaatttttt ttaccgctcc tgttgctgat ggcatcgtgc aaaaaagaca cggacatcgg
    12721 ggcaaatctc cagccacaga atgaactgat caatctgctc acgacggata caacatcgct
    12781 gttgacgtac accgttcccg aagattctgt gcgcagcgac aagtcgccta ccgtacagct
    12841 cggctgcctg aacgacggta actttggaaa aacgacggca tcgctctttt cccagttcgt
    12901 aattccgaat aacctgacgg atattaattt cggaacagga gctgccgtgg attccctggt
    12961 gctttacatg cgttacgatt tcgactatta cggcgatacg atgacaacgc agacgttcaa
    13021 cgtgtaccag atgcaggaca gcatttcctg ggacagcgcc tactattcca accagcgtaa
    13081 atcctgtttc ccgggttcga tcgggtcggc gtcattcctg cccacgccaa ggtcgaagtc
    13141 tgttgtggag ggagatacgg tttcaggcgt acttcgcatc cctatttcaa acgcgtgggc
    13201 gcagaacttc atcaactggg cggcgggtgg aaacatgcac agcaacagca cgctcctcca
    13261 ggttttctat gggttgtgga tcgttcccga cagtaatctt gcaagcggcg gggcgctgct
    13321 gcgcttcaca ccccaggata cgctcacggg actgaaaatg tattaccaca acagtaccga
    13381 tacggcttcg ttcacatttg ccattaatgc aggaaccgcc tattacagct atttccagca
    13441 taatttcaac ggtatttcct cttcatcacc gctctacaac cagctgcaca atcccggagc
    13501 caattccgat ccggaggttt acatacaatc cgctgccgga ctgaaaacga agatcgagtt
    13561 tccgttcctc agtgactgga tgagctcgac cgcgccaata gccgtgaaca aggcggagct
    13621 gatcatcaag gcagacccca cgcaggcttc caacgatctt ccgatcaaca agcagcttta
    13681 ccttgtttac ctggattcgg cgggcaactc gtatgttctg ccggacatgt tcgagaacag
    13741 cacatactat gggggatcgg tcattaccac gacaagcgaa taccacatca accttgcgcg
    13801 gtacttccag gaaatagtga ccggacgcac cggcaactac gggatgtacc tcaaggaaat
    13861 ttctcccgtc atcgaaggcc gccgggcggt tctcggcagc ccgaattcca caggttacaa
    13921 aatgtacctg caccttgtgt atacaagggt taactgataa acactttttc tcatgtgtgg
    13981 cattgtagca tatatcggaa cacgcaaggc gtacccgata cttgtgaaag ggttgcaccg
    14041 tctcgagtac cgcggttatg acagcgccgg gatcgcactc attcacgaaa gcctgaacgt
    14101 ttacaaatgc aaggggaagg tcgaagacct tgaacgcttt gcatccggtc atgatacgga
    14161 agggaccgtg ggaatcggcc acacacgatg ggcgacgcac ggcgagccga acgacatcaa
    14221 cgcgcaccct catttttccg ggagcaaaaa gctcgtgatc attcacaacg ggatcatcga
    14281 aaactatgcg ccgctgaaag ccgagctgat caagcgcgga cataaattcg aaagcgatac
    14341 agacacggaa gtgctggccc acctgatcga ggacatccag ctgcgcgaga aaaccgacct
    14401 gttcgaggcg gtgcgcatcg cgctcaacgg cgttgtgggc gcctacgcga tcgttattct
    14461 ttcgaaagaa aatcccgacc agctgatcgc tgcgagaaaa agctctccgc tggtgatcgg
    14521 catcggcaat gatgaatatt tcgtggcgtc ggatgccaca ccgatcgtgg aatacacgaa
    14581 aaacgtcgtt tacctcgagg acgagcagat cgcgatggta aagcggggcg aagatcttct
    14641 catccgcacc atcaagaaca aaaagatcac gccgtacata catgagctgc agatccagct
    14701 cgacgcgatc gagaaaggcg gctatgacca tttcatgctg aaagagatct acgagcaacc
    14761 ccgttcgatc aaagacagca tgcgcgggcg actgaaccca acggaaggcg ttgtcgcgct
    14821 ggggggaatc cgcgattatc tccagaaatt cacgaccgct aaacgtgtca tcatcatcgg
    14881 ctgcggaaca tcctggcatg ccggtcttgt agccgaatat cttttcgaag agcttgcccg
    14941 catacccgtg gaggtggaat acgcctccga attccgttac aggaacccgg tcatttacga
    15001 agacgacatc gtgatcgcca tttcacaatc gggagaaaca gcggatacgc tggccgccat
    15061 cgagctggcg aaatcgaaag gcgcaacgat tttcggtgtg tgcaatgtcg tcggttcgag
    15121 cattgcgcgc gctacgcatg cgggttcgta cacgcacgcc ggcccggaga tcggggtagc
    15181 gtccaccaaa gcatttacgg cacaggtcac cgttctcacg ctgatggcgc tcctgctcgg
    15241 tcacaagcgc ggcatgatca gcgaacggca ttaccacgag ctcctcagcg agcttgaagc
    15301 cataccgaac aaagtggaaa agctgctgac gacgaacgac aaggtgaaat acctcgcttc
    15361 ggtgtacaag gatgcaagga attttcttta tctcggtcgc ggctattcat tccccgtcgc
    15421 gctggaaggt gcgctcaagc tcaaggagat ctcttacata catgcagaag gttatcctgc
    15481 tgcagaaatg aagcacggcc cgattgcgct gatcgatgat gaaatgcccg tagttgtcat
    15541 tgcgaccaag ggtgcttcct atgaaaaagt cgtgagcaac atccaggaag tgaaggcccg
    15601 caaaggcaag atcattgcgg ttgttacgga gggcgatgaa acggtccgaa aaatggcgga
    15661 ccatgtgatc gagatccctg aaacggacga gaaacttgtt ccgctgctgg ccgttatacc
    15721 gctgcagctc ctcgcctatc acattgcggt gatgcgcaag tgcaatgtcg atcagccgag
    15781 aaacctcgcc aaatcagtta cagtagaata acgatcccaa cgccctcacc gggcgttttt
    15841 tcatttgacc cacataaaca cgttcgggtt cccgtgaaat gattcgaact cttcatcgct
    15901 ggcggaaacg atcgcgataa aatcgagaag aggaagcagc ccgaaaccgc ctgccgtaac
    15961 aatataaacc acgggtacgt acggcgcggt tcccaggtag atccggtgaa gcccgagaat
    16021 tccgaacgga acagggaaag cgagaacagc tgcgatgaaa cgatcatttg cagcacgttt
    16081 tgccgtcgcc ttcacctgca tcgtatcgcg gctcacgcca atcgccgacg tatcgatgac
    16141 gagcgtatcg tgcaggatga tgcacggcac ccggaccggt tgtttttcat tggcgaaaac
    16201 agcggagcaa aacaggagcg cgacaagaag gaagcggaat tgcatgagac aaaattgcgt
    16261 aaaaaaaaac ggggaacccg gaagctcccc gcaatgaaaa agtgaattaa tgttatttcg
    16321 ttcccccgaa gcgggcattg agtccgaccg aaagataata cggaggaccc gcggcaatct
    16381 cagcattcac gccgaggttg ggaatgaaga aataacgcac cccgaaaagt gcctgcggcc
    16441 acaaatgcgg cttgaatttg ctgagatcga acgtctggtc gacatccgca atgttgctgc
    16501 tgctgctcca gtaggaatag cccaggcgga tcccgaaata cggatcgatg tcgtcgttat
    16561 caccgaaatg aaaaagagcc cgcagcgccg tattctggcg tgtaatgcgg tagtgaaaat
    16621 cgcccgtatg ggtgatgccg ttgttgtcgg tataggtaga ccagtgggca tttgcgcttt
    16681 ggtagaaatg cgaaacgccg agactgaacc tgtcggtgac accgacatcg accgttcccg
    16741 aaagcccggg cgtaacgctg gtgctcaggc cgacaatgcc cctatcggcc acattgaaag
    16801 ccgtgttcat tacgagaccg acaagactga acgcgcaatt cgccccgatg acaacctggc
    16861 cctggtattt ctgagcctgc attgttttcg ggagtccggc aaatacaaat gcaaaaagaa
    16921 gtgccgcaga aaaaatggat tttttcatat cagtggtggg ttggtttatc aggctgtttg
    16981 caaacgacgt gccacctctt cccagttgat cacattccag aatgcggaaa tgtaatccgg
    17041 gcggcggttc tggtaattga ggtaatacgc gtgctcccat acatcgaggc cgaggatagg
    17101 tgttcccttc acgtctgcaa tgtccatgag cgggttatcc tggttcggtg tggagcagat
    17161 cgccagcttt ttatcagcgc cgacaatgag ccaggcccag ccggaaccga agcgggttgc
    17221 ggctgcagaa gaaaactgcg ttttgaattt ttcgaaatcg ccgaaagcag cgttgatggc
    17281 cgaagcgaga tcacctgaag gagcgcctcc gccgttcggt ttcatcacct gccagaacag
    17341 cgaatggttg tagtgcccgc cgccgttatt ccgtacagcg acggcatact ttgaaatatt
    17401 cctgcagata tcttcgatgg acattttttc agcgtccgtt ccagcgatcg cattattgag
    17461 gttggtaaca taagcctggt gatgcttcgt gtggtgaatt tccatcgtgc gtgcatcgat
    17521 gttcggctcc agtgcgttat aggggtaagc cagtttggga agttcaaaag ccataatttt
    17581 tgattttgaa agtgaaacaa aattgtattt tcaaatataa caaatgcagc gacggcgatt
    17641 cttaaaaaga ggaattaata ttttccttaa ttttatcctc attacaatca aaaaaacgca
    17701 caatgaaaaa agtatttgcc attgcattcg ccggggcggt gatcgccggc ttcgcttcat
    17761 gcggatcttc cgacgcggac aagaaagctg attcggctaa cgctgttcag accaaaaaag
    17821 aaatggatga aacgggcgac cacatgatcg attccatgaa ccgcgccaac gccgtgatgg
    17881 actccacaaa taaagcgggt gcaaaaacag ataccgcgaa gagcgcgggc aaataaacag
    17941 catccgtctg cacataaggc aaagccgttc cccgcacagg gacggctttt tctgtttaca
    18001 aagatttcat ggctgccgca atggcggcgt cttcatcatt ccagcagagg taataacctt
    18061 cgtctcccca gatgctccgt gcaacggccg ctttcaggta acggcggatg agcgcatcgc
    18121 tgcgtttttc ctgtgcgtcg ttctttttga tcccattctg cgcggcgatc agctccaggc
    18181 cattcaccat ttcatctgaa acagaaaaat tcctgcggaa atcctcccgc ccgttcttca
    18241 ccagcgcatc ccggttgtgc gatgcgaaat tcatggccca caatgaaaaa atatcatgac
    18301 cgaacaatgc ggaaagataa tccgatccac ccgccgtatc gagcgggatg aaaatatccg
    18361 gcatgatgcc gccgccgccg taaacggttt ttcctcctgg tgttttgtat ttcagtgaat
    18421 cgggaaattt aatgctgtcc ttgttcagca gctcgccgtg cttgtaacgg tgctcttcgt
    18481 cggattcgta attttccaat cctttgtcgt acggtttctg gatgcagcgc cccgtcggcg
    18541 tgtaataccg cgcgatcgtc aggcggaaac ccgatccgtc cgtcagatgt ttttcctcct
    18601 gcaccagtcc tttcccgaac gtcctcctgc caatgatcac gccgcggtcg ttgtcctgta
    18661 tggcgcctgc aaggatctcg ctcgcagacg cggagttttc atcgaccagg atggccagcg
    18721 gcatcttttc gagcttccct ttccgcgttg caacgacgcg gtgttcccca tccgttcttc
    18781 cttttgtgta cacgatcagt ttcccttcgg gaaggaattc atcggcgata tcggtagctg
    18841 cgtccatgta tccgccaccg ttcccgcgca gatcgagcac cattttcttc atcccttttg
    18901 cgagaaggga atccgccgct tccgtaaatt cccggtaact cgtttccgca aaacgtgaaa
    18961 gacggatgta gcccgtttca ctgtcgagca tgtattttgc ctcgacactg taaatgggaa
    19021 cgctcccgcg cgtgatctcc acgacaaaat cctttgccgg gaaaggacgc cggacggaaa
    19081 tgcgcacctt cgttccttca ggaccgcgca gcatagtgcg gatctttttg tccgaaatat
    19141 tttttcccgc gatgaccgta tcgttcgctt tgaaaatgcg gtcgccggga agcaggcccg
    19201 aatgttcgga aggtccgccg ggaacaacgg ccatcacca
//
LOCUS       DASTSF010000042         8395 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1978622,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000042 DASTSF010000000
VERSION     DASTSF010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 8395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8395
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1978622"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(264..1478)
                     /locus_tag="VFU15_01220"
     CDS             complement(264..1478)
                     /locus_tag="VFU15_01220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716419.1"
                     /translation="MKTFLTVLLCVFFLTGVRSQAPSRQDILRQGIARCTATCVSYVY
                     DEDGPTVPTPLSGRRLLVKRDPVSTWISVYTYNDSGYCTTLEWIQIFGKDTMMDQVRN
                     FLYDAKGNQSEVSGHTILWTHNPWIKGPDSLQTDRIDDTTYGRGNYRVRKSVENRNGV
                     VSVYGDSTVTPASCHCNVIYHFSGRNVYNEGGNRWDSLNRNLFYEYIEQYPGGGSRET
                     DSVVWLPHGMKKDFWSSYPLSNSKPNSVIVTTRDSAGNVICEERITDTDHSVDSVIYN
                     DKGSIIRKAEYDGNGKKIAGENYFYRNDTLLLVKKQSGNSYSEIHYGVTRKNNQVTYD
                     IRTFETDRQDYAAVAKNWNANAKGYTQREIQVYDVNGNLLSDKIYDKDGLPVNKTFYT
                     YETRSFRLQPQK"
     gene            1655..2569
                     /locus_tag="VFU15_01225"
     CDS             1655..2569
                     /locus_tag="VFU15_01225"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004570899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HEU4716420.1"
                     /translation="MKITPQKVSEIASVIGAKFVGDPAHLVTGLNEVHRVEAGDLMFV
                     DHPKYYDKALKSAATTILINKEVECPEGKALIFSDDPFRDYNKLVLHFQPVSHSLKPV
                     SDSAVVGAGSVIHPGVYLGNNITVGTDCVLMPGVVVYDNCVIGNNVRIHANSVIGGDA
                     FYYKKRPEKFDKMFSCGRVVIHDDVEIGACCTIDRGVSADTIIGRGTKMDNQVHIGHD
                     TVIGEMCLFAAAVAIAGVVTVEDRVTLWGQVAVTSDVVLGAGAVVLGASGVSKSLEGG
                     KTYFGSPAEEARGKWKELAVLRRLAEGK"
     gene            2693..3940
                     /locus_tag="VFU15_01230"
     CDS             2693..3940
                     /locus_tag="VFU15_01230"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HEU4716421.1"
                     /translation="MPLKEKAGTESRSRTPRIREFKLNALLEVTKAINNNLSTADLLK
                     TYEDIIREKLKIGKLVLFNHDGKKWNCLLSFGVDAQMTSLDVEKEFKGIREISTIDMD
                     SSGDHLGKSFEIVIPVFHKSHPLAYVLIGDLDEDESGISAAIKHLPFVQTLTNIIMVA
                     IENKKLAKENLRRAAMHRELELAWEVQRMLFPEKLPHNEYLDMDALYLPHQQVGGDYY
                     DFIPINENEVAICVADVSGKGVPAALLMSNFQANLRILLHHIPNLPDLIRELNTKVSV
                     NAKGEKFITLFIAKYNTVTRVMHYINAGHNPPLVIDDDGANLLTVGCTGLGMFDEIVK
                     VKEGIVNVSHGALLLCYTDGLVETHNDKDEDFGLQRLQQIAMKNRHLPPAELNALILD
                     SLNKHRGDVPYIDDIALVTARLV"
     gene            complement(4169..5650)
                     /locus_tag="VFU15_01235"
     CDS             complement(4169..5650)
                     /locus_tag="VFU15_01235"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEU4716422.1"
                     /translation="MLRILENKKHLWLAYGLLALYLAVNAWCMAHEFFWLNLLPPVLI
                     VVLVAVTSLDKLLLFCVFLTPLSINLSSSEIHLGLSLPVEPILAGTLFLFTLRLIYDA
                     RFDKRFILHPVTIAILANLAWIFITSMTSSMPLVSFKFLIARLWFIVPFYFIGVNLFR
                     DFKNVRRFLWLYSIPLTFVIGYTLYWHMKYRFSEDAAHWVMSPFYNDHTAYGAALAMF
                     YPALIAFSFGKQYFSQVRVLSVFLLGAFTLALIFSYTRAAWVSLAGALVLYLIIVFRV
                     KFRYIALALLAGSFYLYMSWGDILMKLEQNRQDTSNDLSKHLESISNISTDASNLERL
                     NRWNSAFRMFSERPLFGWGPGTYSFQYAPFQLSSEKTIISTNAGDLGNAHSEYIGPLA
                     ESGLPGMLTFLAVAVTMIWTGWRLYYRLPKGRERAVLLSVVLGLVTYFIHGFMNNFLD
                     TDKASIPFWGFAAMLVAADLWYPKKENGEQPRELGENREEEKK"
     gene            complement(5628..6596)
                     /locus_tag="VFU15_01240"
     CDS             complement(5628..6596)
                     /locus_tag="VFU15_01240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716423.1"
                     /translation="MEDLNNTDLWALAVRRKWMLLAIAFLGSVLGFVFSGSTFIRPKY
                     KSVAVVYPVNIIPYSLESPTEQLLQLFNSADVRAMMVKKYDLAKRYGIDTTMSSGKTR
                     LFNEYDDNVNIRKTEFESIKIDICDENPEVACDMVNGLIHFTDVKARNLQRQKTREVV
                     KIFRDQLDFKQHQLDSLDRIMDQLRVRYGLLDYAAQTKELTKNYLKMVSSGTPADRLR
                     PVDTLMRNLEEKGGDLISVSNQVSSLQDSYNSIKSDYDKALSDLTKELTYSNVVTRPF
                     PADSKSYPVRSLVALISAISSLMLGMIFFAFLDNKSRRQDAPNSGK"
     gene            complement(6789..7859)
                     /locus_tag="VFU15_01245"
     CDS             complement(6789..7859)
                     /locus_tag="VFU15_01245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716424.1"
                     /translation="MEQNTGEKTISDSHLSEKKGGAFFDSTSLLEFFWYWRWQLIVIG
                     LAAAIISSVVSFLIPERFKSTVIMFPVASNAISKALLIEDVSDKQDVLQFGEEEQAEQ
                     MLQILNSDEIRMRICDKYDLMKHYHIDPTDKYKNTKLYETFYDNVSFKRTEYLSVKIE
                     VLDRNPDTAAYIANDIAALLDSTKTRMQHDRASQALAIVEHEYLDKKAEVELMTDSIK
                     ALNEKGIYDYESQSEVTSEQYAIALSKGDEKAIQRLGDQLKIISTYGSAYMSVRENLY
                     IQREQLNVLKKRYEEAKVDADESLTYKFIVNRAFPAERKSYPVRWLIVSVSTASSLIL
                     GVLLLILYANIRMIRLGKTKES"
     gene            complement(8008..>8395)
                     /locus_tag="VFU15_01250"
     CDS             complement(8008..>8395)
                     /locus_tag="VFU15_01250"
                     /inference="COORDINATES: protein motif:HMM:NF026018.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HEU4716425.1"
                     /translation="LREYNIMAFCGMVFNIGMNIFFIRVMHFEAIGTAISSLCTQMLT
                     ALAQVLIVQSKFHFRINWRLLTTLFVYVAGVIAIANLCHSYLLHKASTSGWVPGFLVM
                     GAVSVAWALAIRLISVKGMFRMLKYG"
BASE COUNT         1920 a         2160 c         2332 g         1983 t
ORIGIN      
        1 aaaacggttt cagtttatcg cgtgtggatc atcattccaa cttttaacct gaatcgtctt
       61 cattttcctt cgtgcccttt gtgaaaagct ttgtgttctt cgtggttaaa acggtttcag
      121 tttatcgcgt gtggatcatc attccaacct tccaaccttt cagctttcca actttgaact
      181 gaattatttt cgttttcctt cgtacccttc gtgaaacgct tggtgttctt cgtggttaag
      241 gcaattcaca gcttcccgtc tcttcatttt tgcggctgca accggaatga tcttgtttcg
      301 tacgtataaa acgttttgtt taccgggagg ccgtccttgt cgtaaatctt gtccgaaagc
      361 aggttaccgt ttacgtcgta aacctggatc tcgcgctgtg tgtatccttt cgcatttgca
      421 ttccagtttt tcgcgacggc cgcataatct tgccggtccg tttcgaacgt gcgtatgtca
      481 tacgtcacct ggttgttttt ccgcgtgacg ccgtaatgga tctcactgta agaatttccg
      541 ctctgtttct tcaccaggag cagcgtgtcg ttccggtaaa aataattctc gcccgctatc
      601 ttcttcccgt ttccgtcata ttcagctttc cggatgatgc ttcccttgtc gttgtaaatg
      661 accgaatcga cgctgtggtc agtgtccgtg atgcgctcct cgcagatcac gtttcctgcg
      721 ctgtcgcgcg tggtgacgat cacagaattg ggtttgctgt tgctgagcgg atacgacgac
      781 cagaaatctt ttttcatccc gtgcggaagc cacacgacgc tgtccgtttc gcgcgatccg
      841 cccccgggat actgctcgat gtattcgtaa aaaagattcc tgttcagcga atcccatcga
      901 ttgccacctt cgttgtaaac atttctcccc gaaaaatgat agatcacgtt gcaatggcag
      961 gacgcaggtg taacggttga atctccgtaa acagagacaa caccgttcct gttttccacg
     1021 gatttgcgca cacggtaatt gccgcgcccg tatgtcgtgt cgtcgatcct gtcggtctgc
     1081 aggctgtccg gacctttgat ccagggatta tgcgtccaca ggatcgtatg gccggaaacc
     1141 tccgattgat ttcctttcgc gtcgtacaaa aaattgcgca cctggtccat catcgtatcc
     1201 ttcccgaaga tctgtatcca ttccagcgtg gtgcaataac ctgaatcgtt gtacgtgtaa
     1261 acggagatcc acgtggaaac cgggtcgcgt ttcaccagca aacgccggcc ggagagcggt
     1321 gtgggcacgg tggggccgtc ttcatcgtaa acatagctca cgcaggttgc cgtgcatcgc
     1381 gcgattccct gccgcaggat atcctgccgt gaaggcgcct gcgatctcac accggtaagg
     1441 aaaaaaacac aaagaagcac ggttaaaaat gttttcatct ggcagggccc tgggtgcatg
     1501 gcaatttccg atccgggtga tgcgcggcaa aaaacatcag gagcaaaacg gggcgaacgg
     1561 gcatgaatga gggttgatca aatttaagga atgcgggaat gaaaccggcg gcaaccgggt
     1621 gttcttttcc gtaatttcgg gcaaacgttt ttccgtgaag atcactccgc agaaagtttc
     1681 cgagatcgcc tccgtcatcg gtgcaaaatt cgtgggagat cctgcgcatc ttgtcacagg
     1741 tctcaatgaa gttcaccgcg tggaagccgg cgatcttatg ttcgtcgacc acccgaagta
     1801 ttacgataaa gcgctgaaat ccgcagccac caccattctc atcaacaagg aggtggaatg
     1861 cccggaagga aaagcgctga tcttttccga tgatccgttc cgcgattaca acaagctggt
     1921 gctgcatttt cagccggtga gccattcgct gaaaccggtg agtgattcgg ccgtcgtcgg
     1981 tgcgggaagc gtgatacacc ccggtgttta ccttggcaat aacatcacgg tcggaacgga
     2041 ttgcgtgctt atgcccggcg tggtggtata cgacaactgc gtgatcggga acaatgtgcg
     2101 catacacgcg aattccgtca tcgggggaga tgcgttctat tacaaaaagc ggccggagaa
     2161 attcgataaa atgttttcct gcgggcgcgt cgttattcat gatgacgtgg agatcggtgc
     2221 ctgctgcacg atcgaccgcg gcgtaagcgc tgatacgatc atcgggcgcg gaacgaaaat
     2281 ggacaaccag gtgcacatcg gccacgacac ggtgatcggc gagatgtgcc tcttcgccgc
     2341 cgctgtggcg atcgccggcg tggtgacggt ggaagaccgc gtgacgttgt ggggacaggt
     2401 ggccgtcaca agcgatgtcg ttctcggtgc gggagctgtc gtgctgggcg cttcaggggt
     2461 ttcgaaatcg ctggaaggcg gtaaaaccta tttcggaagt cctgccgaag aagcgcgtgg
     2521 caaatggaaa gagctggcag tgctgcgccg tcttgcggaa ggaaaataat aatttcatta
     2581 tacgggaata atgccaccgc cggggacgcg gaatctcaac cgttgatcgg ttcaatcaca
     2641 gcgtttgtct aaaaaatccc cggacggggt tctgaacgtt taatttagcc tcatgccgct
     2701 gaaggaaaaa gccggaacag aatcgcgaag ccggacaccg cgtatccgtg aattcaaact
     2761 gaacgcgctg ctggaagtga cgaaggcgat caacaacaat ctttccaccg ccgacctcct
     2821 gaaaacgtac gaggacatca tccgcgaaaa gctgaagatc ggcaagctcg tgctgttcaa
     2881 ccacgacggg aaaaaatgga actgcctgct cagcttcggc gtggatgcgc agatgaccag
     2941 cctcgatgtc gagaaggaat tcaaaggcat ccgcgagatt tccaccatcg acatggattc
     3001 ttccggcgat catctcggga aatcgttcga gatcgtcatt cctgtttttc acaaatcaca
     3061 cccgctggcc tacgtgctga tcggcgacct ggacgaagat gaaagcggca tcagcgctgc
     3121 gatcaagcat cttccgttcg tgcagacgct caccaacatc atcatggtgg cgatcgaaaa
     3181 caaaaagctc gcgaaggaaa acctgcgccg tgcagcgatg caccgtgagc tcgagctcgc
     3241 ctgggaagtg cagcgtatgc tcttcccgga aaagctcccg cacaacgagt acctcgacat
     3301 ggatgcgctg tacctcccgc accagcaggt aggcggtgat tactacgatt tcattcccat
     3361 caacgagaac gaagtcgcca tctgtgtcgc cgatgtttcc gggaaaggcg ttcctgcggc
     3421 gctgctcatg tcgaacttcc aggccaacct gcgcattctc ctgcatcaca tcccgaacct
     3481 tcccgatctt atccgcgagc tgaacacgaa agtttccgtg aatgcgaaag gcgagaagtt
     3541 catcacgctt ttcatcgcga aatacaatac cgtcacacgc gtgatgcatt acatcaacgc
     3601 agggcacaat cccccgctcg tgatcgacga cgacggcgcg aacctgctga cggtgggctg
     3661 cacagggtta ggcatgttcg acgagatcgt gaaagtgaag gaaggaatcg tgaacgtttc
     3721 acacggcgca ctgctgcttt gctatacgga tggcctcgtg gaaacacaca acgacaagga
     3781 cgaagatttc ggactgcagc gcctgcagca gatcgcgatg aaaaaccgcc atcttccgcc
     3841 cgcggagctc aatgcgctca ttctcgacag cctgaacaag catcgcggcg atgttcccta
     3901 tatcgatgat atcgcgctgg tgacggcgag gctggtgtag gtaaaagcga gcgataaaca
     3961 gtttcaattc cttttgggta atcccttatc tggcttggct ttgagccact ttttaccaac
     4021 tattttttgc tattaaatct tcaaagtacg tgttgaaaag atgcttcggt caaacgcgca
     4081 aaacgtcagc ggcggaagca atacgttcga aaaataattc ctgaactttt gggcagcctc
     4141 ttggcctcta acgcgaagct cctgcccgtt attttttttc ttcttcgcgg ttctctccca
     4201 actcccgtgg ctgttccccg ttttctttct tcgggtacca caggtccgcc gccacaagca
     4261 tggctgcaaa tccccagaaa ggaatggatg ctttgtccgt gtcgaggaag ttgttcatga
     4321 agccgtggat gaagtacgtg acaagtccca gcacgaccga aagcagcacg gcgcgttccc
     4381 ttcctttcgg taagcggtaa tacaatcgcc agccggtcca gatcatcgtg accgcgacgg
     4441 cgagaaaggt gagcatgccg ggcaggccgc tttccgccag cgggccgatg tattcgctgt
     4501 gcgcgttgcc gagatcgccc gcattcgtgc tgatgatcgt tttttcactc gacagctgga
     4561 acggtgcata ctggaagctg taggttccgg gtccccagcc gaacagcggc cgctcgctga
     4621 acatgcggaa cgccgaattc caacggttga gccgttcgag gttcgacgcg tcggtggaaa
     4681 tattggaaat ggattcgagg tgcttgctga ggtcgttcga cgtatcctgc cggttctgct
     4741 cgagcttcat gagaatgtcg ccccacgaca tgtacaggta gaacgagccg gcgagcaggg
     4801 cgagcgcgat gtagcggaac ttgacgcgga aaacgatgat gaggtacagc acgagcgcac
     4861 cggcaaggct cacccacgcg gcgcgggtgt aggaaaagat gagcgcgagc gtgaaagcgc
     4921 ccaggaggaa cacggagagc acgcgcacct ggctgaagta ttgcttcccg aaggaaaaag
     4981 cgatgagcgc gggataaaac atggccagcg cagcgccgta tgccgtgtgg tcgttataga
     5041 aaggcgacat cacccagtgg gcggcgtctt cgctgaagcg gtatttcatg tgccagtaca
     5101 gcgtgtaacc gatcacgaac gtgagcggaa tggaatacag ccagaggaaa cggcgcacgt
     5161 ttttgaaatc gcggaaaagg ttgacgccga taaaatagaa cgggacgatg aaccacaggc
     5221 gcgcaatgag aaacttgaac gaaacaagcg gcatggagct ggtcatgctc gtgatgaaga
     5281 tccacgcgag gttggcgagg atggcgatcg taacgggatg aagaatgaac ctcttgtcga
     5341 accgtgcgtc gtagatgagg cggagcgtga aaaggaaaag tgtcccggcg agaatgggtt
     5401 ctaccgggag gctcagcccg agatggattt cgctgctcga caggttgatg gaaagcggcg
     5461 taaggaaaac gcagaagagc agcagcttgt cgagcgaggt gaccgcgacg agcacgacga
     5521 tgagcacggg gggaagcagg ttcagccaga aaaattcgtg cgccatgcac caggcattca
     5581 ccgcgaggta aagcgcgagc aggccgtagg caagccagag gtgtttctta ttttccagaa
     5641 ttcggagcat cctgcctgcg tgacttgtta tcgaggaaag cgaagaagat catgcccagc
     5701 atcagcgacg agattgcgct gatcagcgcc acgagtgaac gcacaggata ggatttcgaa
     5761 tcggcaggga acggacgcgt caccacgttg gaatacgtta gctctttcgt aagatcgctg
     5821 agcgctttgt cgtaatcgct tttgatggag ttgtacgaat cctgcaggct gcttacctgg
     5881 ttggaaacgg aaatgagatc gccgcctttt tcctcgaggt tgcgcatgag cgtatcgaca
     5941 ggacgaagcc ggtcggccgg tgttcccgag ctgaccatct tgaggtaatt cttcgtcagc
     6001 tccttggtct gcgccgcgta atcgagaagc ccgtaacgta cgcgcagctg gtccatgatg
     6061 cggtcgagcg aatcgagctg gtgctgcttg aagtcgagct ggtcgcggaa gatcttcacc
     6121 acttcccgcg tcttctgccg ctgcaggttc cttgccttga cgtcggtgaa gtgaatgagc
     6181 ccgttcacca tgtcgcaggc gacttcagga ttttcatcgc agatatcgat cttgatggac
     6241 tcgaattccg ttttgcgtat gttgacgttg tcgtcgtatt cgttgaacag gcgggttttc
     6301 cccgagctca tcgttgtgtc gatgccatac cgtttcgcga ggtcgtattt tttcaccatc
     6361 atcgcacgga catcggccga gttgaaaagc tgcagcagct gctctgtggg tgattcaagt
     6421 gaatacggga taatgttcac cgggtaaacc accgccacgg atttgtattt cggccggatg
     6481 aacgtcgacc cggaaaatac gaaacccagc acgctcccga ggaaagcgat cgccaggagc
     6541 atccactttc ttcttacggc aagcgcccag agatccgtat tgttgaggtc ttccatttgt
     6601 attcctgttg ttgttcccgg agcggtcttt tcaggggatg aaaaattctt tctgatcagc
     6661 tccttcagta cagcaacaat tttcggtttg atattctgtt ttgtaccggc gtaataaaga
     6721 atggtccact cttttctgcc ggctggtttt tcgttttctg ctccggacgg gaacgccccg
     6781 gcttccagtc agctttcctt ggtttttccc aaacggatca tccggatgtt cgcgtaaaga
     6841 ataagcagca gcacgccgag gataagtgag ctggccgtgc tcacgctgac gatcagccag
     6901 cgtacgggat aggatttccg ctctgcagga aatgcgcggt tgacgataaa cttgtacgtc
     6961 agcgattcgt ccgcatcgac tttcgcttct tcgtaacgtt ttttcaggac gttcagctgc
     7021 tcgcgctgga tgtacaggtt ctcgcgcacg gacatgtacg cgcttccgta tgtggagatg
     7081 atcttcagct ggtcgccgag acgctggatg gctttctcgt cgcctttcga aagcgcgatc
     7141 gcgtattgct cgctcgtcac ttccgactgt gattcgtaat cgtaaatgcc tttttcgttc
     7201 agcgctttga tcgagtccgt catcagctcg acctccgctt tcttgtcgag gtattcatgc
     7261 tcgacgatcg cgagcgcctg tgaggcgcgg tcgtgctgca tgcgcgtctt ggtggaatcg
     7321 aggagagccg cgatgtcgtt cgcgatgtat gcggccgtgt cgggattgcg gtccagcact
     7381 tcgatcttca cggaaaggta ttcggtgcgc ttgaaagaaa cgttgtcgta gaacgtttcg
     7441 tagagcttcg tgttcttgta cttgtccgtc ggatcgatgt ggtaatgctt catcaggtcg
     7501 tacttgtcgc agatgcgcat gcggatctcg tcggaattca ggatctgcag catctgttcg
     7561 gcctgctctt cctcaccgaa ctggagcacg tcctgcttgt cggaaacatc ttcaatgagc
     7621 agcgctttgg aaatggcgtt cgaagcaacc gggaacatga tcacggtcga tttgaaacgt
     7681 tcgggaataa ggaatgatac gacagacgaa ataatggcgg ccgcgagacc gatcacgatc
     7741 agctgccagc gccagtacca gaaaaattca agaaggctgg tcgagtcgaa aaatgcaccg
     7801 cctttttttt cagacagatg tgaatcgctg atggtctttt ccccggtatt ctgctccatt
     7861 ggaattggta aaacttagga tggacgccaa aagtaagaaa aatcggtagc gcacagggag
     7921 gacggccctg tttcctattg gaattcctgc aaaaacagtg tgttaaacta ttggaaagca
     7981 gttttttaac aatgaaaaaa cgccgcttta gccgtatttc agcatgcgga acatgccttt
     8041 tacgctgatg aggcgtatcg ccagcgccca ggccacggaa acagcgccca tcaccaggaa
     8101 accgggcacc cagccgctcg tgctggcctt gtgcagcagg taggaatggc agaggttcgc
     8161 tatggcgatc acgcccgcga cgtacacgaa aagcgtcgtc agcaggcgcc agttgatgcg
     8221 gaaatggaat ttcgactgca cgatgagcac ctgtgcgagg gccgtgagca tctgcgtgca
     8281 aagactggag atcgccgtcc cgatggcttc gaaatgcatg acgcggatga agaagatgtt
     8341 catgccgatg ttgaacacca tgccgcagaa ggccatgatg ttgtattccc tgaga
//
LOCUS       DASTSF010000043        11907 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1530551,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000043 DASTSF010000000
VERSION     DASTSF010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 11907)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11907)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11907
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1530551"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2655)
                     /locus_tag="VFU15_01255"
     CDS             complement(<1..2655)
                     /locus_tag="VFU15_01255"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU4716426.1"
                     /translation="MKKFRSYHFAFLLLTMAFIVACSTTKNTFVTRSYHNMTSRYNGY
                     FYAKEAMKSAMLTVDKSYVDDYSQLLPLFKLPITPETKACYTDLEKAIKKSQNVISRH
                     TITNKSGIEIPGAVKWIDDNYLIIGQAHYYKGEYLAALDMFDYIIDTYSKFPIRFDGM
                     LWKARAQIQLGAYKDAESELDVVANNKSCPPRLNADVKATYADLYMHTGNYPNAIKYL
                     EQAILLTKKKSTRARYLFVLAQLYEKVGKEKDAYATYGKVIALHPQYDMLFNAKLNRA
                     RLSASDVKNRTAAKKELQKMLTDEKNIEFQDQIYYTLGQLEEKSGDKAGALAYYKLSV
                     AASVGNSRQKAVSYLAIGDLYLADTDYKGAGAYYDSTLQFLPRDYPDYDGVADKRKSL
                     DDLVRFLDIISNEDSLQNIVNRYGSDTTALYPFIDGLIAKQKEEDDRKKELQSQQNQS
                     GVGNNNNLANQNNLGSGAAWYFYNPSTVSFGINEFTRKWGSRKLEDNWRRSNKEVVVE
                     DNDNSDNPSDSGSVVKGGSAKAGKNDKYSRDYYIKNLPLTADAKAKSDDRIADAYFNL
                     GSIYKEQMGNTPKAIDAFETLCKRFPDHKYAMPAHYQLYRMYKAQKNSTKAQEHKDYI
                     CSHSPDGEYCRMINDSSFFIKREGKREKMLAFYDSTFITYSHKNYPAVVSMCNQADSL
                     YGTDLKTNEMAAKFAYMRAASLGKTQGIPAMEAALTKILVNYPKDPIIPQVQSLLDAI
                     REQRRDSTGNASNPKKDTSGVKPPAGPDYKFNEKVEYQYMVVVQPGKGDLNNFKIAIS
                     DFNSQMFSSNNLEISTLVLDASHTLIMVQKFPDKSKAMEYYDLLKSRPDIFAWLNQGS
                     YQAMVISTENLPLLLKDKNVDTYKTFFQQ"
     gene            2842..3675
                     /locus_tag="VFU15_01260"
     CDS             2842..3675
                     /locus_tag="VFU15_01260"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEU4716427.1"
                     /translation="MERLRSRYRLVVMNDETFEERLSVRLTPLGFLILIAATTIIMTT
                     LVVSLVAFTPLREYIPGYADVGLQTKLDSLSVRADSLQNSMVARKKYMDNLRIILTGQ
                     DSAEHPKNFRDSTKKYANIKAKPSKEDSALRDEIEDMDEYSLKMDVRHQGISGFFFFV
                     PVKGTITNPFNMAEGHFGVDIVAADENEPIRATLDGTVISAGYTVDDGYVIQVQHSDN
                     LVSIYKHCASLLKKTGDYVKAGDPIAIVGSSGEQIAEPNLHFELWYNGTPIDPQENMV
                     F"
     gene            3766..4950
                     /locus_tag="VFU15_01265"
     CDS             3766..4950
                     /locus_tag="VFU15_01265"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020604827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEU4716428.1"
                     /translation="MSESETRNPKPEIRRLAILGSTGSIGTQALDVVAAHPGHFEVEV
                     LAAGNNAELLVAQAKKFRPNVVVINDTAKLAFVKEALHDDDIKVYGGEEALCDVVQME
                     TIDLVLAGIVGYAGLRSTLAAVKAGKQIALANKETLVVAGEIVTAEARKNGVNLYPVD
                     SEHSAIFQCLAGEFHNPIEKIYLTASGGPFRGKKRDELLHVTKKDALKHPNWEMGAKI
                     TIDSATLMNKGLEVIEAKWLFGLKPEQIDVIVHPQSIIHSIVQFCDGSMKAQMGLPDM
                     KLPIQYALGYPQRLRSDFPRFNFMNYPSLTFEKPDTETFPCLQLAMDALKKGGNMPCV
                     MNAANEVAVQAFLGEKISFLQIAEVISGTMAQSHFLAKPSYEDYVKSDEEARRVAVHF
                     AK"
     gene            5033..5647
                     /locus_tag="VFU15_01270"
     CDS             5033..5647
                     /locus_tag="VFU15_01270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716429.1"
                     /translation="MQKISARFGILSAAFFLFILPASAQLKVKDVDTAYYNKVIATGK
                     KLSMKQDALLGAKAAALPQNFTDTLKKYDWYELDNYSFADKEYRGRFTEEDASNSRQY
                     DFFRYDADGTINTMYLFHTIKQGEYSFYTTTFDKNTAEKLVGVENIRGAAYMVSSAYG
                     EKEWQKIISYKDQVMIKELTRYGKPGETPVKFRIAYMAMTRDDK"
     gene            5929..7323
                     /gene="rseP"
                     /locus_tag="VFU15_01275"
     CDS             5929..7323
                     /gene="rseP"
                     /locus_tag="VFU15_01275"
                     /inference="COORDINATES: protein motif:HMM:TIGR00054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEU4716430.1"
                     /translation="MVKFTLFFFSLSLLIILHEFGHFITARWFKARVDKFYLFFDFLF
                     PFPGIAKFALFKKKIGETEYGIGWFPFGGYVDIAGMTADSEEDKNKKDKDAKEGEEGK
                     DAPEEKKEPKPPGKHEFRGKKPYQRLIILAGGITVNALLAIVIYSMVSWAYGEAYLPV
                     KNAKYGMAVDSVAMGIGLKDGDKLLSVDNKPMESFQDAKVAIILDKAKTIQVDRNGEK
                     VNISVPDGFDQTVVEKDVKVLFDARFPFVVDSIIPGMPAAKSGMMKGDSIVSIDGDTL
                     VYFQQIARKLSGYANKTAHVGIVRNGSWKTIDIAINGEGKMGVLPYAEDRYMTLNTKE
                     YGFFSAWGRGFQRTGEVLVNYVKQLRLLFTKAGAKKIGGFATIGGLFPSTWDWEAFWN
                     ITAFLSVILAFMNLLPIPVLDGGYILFVLWEMITGKKVSDKFMQKALTIGMYIVLGLL
                     IFANGNDILRAFRH"
     gene            7364..8173
                     /locus_tag="VFU15_01280"
     CDS             7364..8173
                     /locus_tag="VFU15_01280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716431.1"
                     /translation="MKAALRSLFCILLLPAITFAQVNTRNDGNDNPPKAPNGKTKVLI
                     IPWEPITFNVSSDVSRAISNETGQKYDQIEESLRKGLVDQLKKSFGPTCSVTSLLDDT
                     AKMKKDLRYTYVVTQRSYTPVNFPLNPTKADSAKLNAPKQGTSNGQIQTTDDETDKFM
                     NCTVLSPNLLGYLHAKYGADYIIFINEIDIDNNFGADPYNTQGKTDYVRDVVVHWTIY
                     SATTEKRVAAGKSKGTFSSATNTPSKISSGAFSAIAKDIYSRYLAGIKPKK"
     gene            8222..8926
                     /locus_tag="VFU15_01285"
     CDS             8222..8926
                     /locus_tag="VFU15_01285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716432.1"
                     /translation="MIRPAFHIFFTAIVFFVTLPSRSQVVICDTMPLAYVSQGLRFSG
                     GFSCADSVITIPIVNNTTTGFAYPLAKIRNTTPLPPGMSLHSSNWQVFASSWNPGDTM
                     PAYICFDVTQPVPVNYTVTFELYVSNFLPLQVDSCVFANVFTINLNPLASAGDGVAMS
                     TGFSFSQDAKNALLVLKNNSGHEMHIRIFSADGKTVSSSSCAPGSSSLSIAGLPPGIY
                     FIGSEEEGCCGKFIVP"
     gene            8997..10148
                     /locus_tag="VFU15_01290"
     CDS             8997..10148
                     /locus_tag="VFU15_01290"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEU4716433.1"
                     /translation="MKLLSTFIVSAVFFFSADAQNVPVFGNEIPVTITGLTFDAMEPF
                     ISPDGNYLFFNSLNDGITTSLYYAVRVNDSVFTYMGALNGADQTVTPRLDAVASCDSV
                     NNFYWTSTRNWPAQYDNFFHGTFNGSDVVNTGRVHGNFYIYTPGWIVMDAAISTDGSL
                     LWYCNAYFNNCSNMPCKAQLGVAAKLNDSTFAKLANSDGLLMNVNDTNYDVYAPNISS
                     DGLELYFTRILKNNPTQTTICVSVRNTLNDTFGLPVVVYGSPLLPEAATLTTDMSRMY
                     YHKKPGSQYQIFLRYRTSPSGIEATDNANPFFIAPNPCHDVLRIFSADGTAPGRVVIY
                     DVAGQIVRTEADKTTLVDISALQAGVYFVSVMTAEKPAVIRIIKTYSGE"
     gene            10141..10602
                     /locus_tag="VFU15_01295"
     CDS             10141..10602
                     /locus_tag="VFU15_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002977977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HEU4716434.1"
                     /translation="MNNEIQTGDFYVAGISVRTTNAGGQSMKDIGELWGRFFAENISA
                     KIPGKVSADIYSIYTDYESDHTGLYTTLLGCRVSSVEDLPEGMTGKKIPGGKCMKFTC
                     KGKIPDVIAAQWANIWNDKTLRRSFTSDYEVYNERAQNPADAEVDIFVAVK"
     gene            complement(10683..>11907)
                     /locus_tag="VFU15_01300"
     CDS             complement(10683..>11907)
                     /locus_tag="VFU15_01300"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HEU4716435.1"
                     /translation="TICSGQPAPIGANAQGGTGSYTYSWSPALGPNSSYTVYPPAPTT
                     YTVHAVDANGCTSPSASVTVDMFSLVPGDLTVSGPASVCQGTQVTISAMLNGNTGPVT
                     WNWNNQPWTTGGPFAVQPSSSTTYQVNVTNICGTVVSQSIPITVHPNPVVTLSPQSGS
                     GCDQVRLQFADNDPANAGCTYSWDFGDGYTAAGSSTYHDYTDDGNYLVTVHIISPFGC
                     TGSTTTTASAIVYQSPHAGFSPSANDVSELEPTILFQNQCSANTIGWHWDFGDNDTSN
                     VPSPSHTYADKGSYNVRLIATSVGGCMDTTEMPVAIDPEFTLFIPNAFTPNGDGKNDV
                     FLAYGNEVSAFHMMMFDRWGNMIFESNDINKGWDGRGNGGSDIAQQDVYVYKIDVTDF
                     RGKLHRYTGHVSLLQ"
BASE COUNT         2714 a         3107 c         3272 g         2814 t
ORIGIN      
        1 ctgctgaaag aacgttttgt aggtatcgac gttcttgtct ttgagcagca ggggcaggtt
       61 ttcggtcgaa atgaccatgg cctggtagct tccctggttg agccacgcga aaatatccgg
      121 ccgcgatttc agcaggtcgt aatattccat cgccttcgac ttgtcgggga atttctgcac
      181 catgatcagc gtatgcgaag cgtcgaggac gagtgtgctt atttcaagat tattggagga
      241 aaacatctgg ctgttgaaat ccgaaatggc gatcttgaaa ttattcaggt cgcctttgcc
      301 gggctgaaca accaccatgt actggtactc cactttctcg ttgaacttgt aatcgggtcc
      361 cgccgggggt tttacaccgg aggtgtcttt tttcggattt gacgcgttcc cggtgctgtc
      421 gcgcctctgc tcacgaatgg cgtcgagcag cgactgcacc tgcgggataa taggatcttt
      481 cgggtagttg acgagaattt tcgtcagcgc cgcctccatt gccggaatgc cctgtgtttt
      541 cccgaggctg gctgcgcgca tgtaagcgaa cttcgccgcc atttcgttgg ttttgagatc
      601 tgtcccgtaa agcgaatcgg cctggttgca catcgagacg acggcaggat agttcttgtg
      661 cgaataagtg atgaacgtcg aatcgtaaaa ggcgagcatt ttttcccgtt tcccttcccg
      721 tttgatgaag aacgaggaat cgttgatcat ccggcaatat tcgccgtccg gtgaatggct
      781 gcagatgtaa tctttgtgct cctgcgcttt ggtgctgttc ttctgcgcct tgtacatgcg
      841 gtacagctgg taatgagccg gcatcgcgta tttgtgatcg ggaaagcgct tgcagagcgt
      901 ttcgaaggcg tcgatggctt tcggtgtatt gcccatctgc tctttgtaaa tggagccgag
      961 gttgaaatac gcgtccgcga tcctgtcgtc cgactttgct ttcgcatcgg cggtgagcgg
     1021 aaggttcttg atgtagtaat cgcgggaata tttatcgttc ttccccgctt tcgcgcttcc
     1081 gcctttcacc acgcttccgc tgtcggacgg gttgtcggaa ttgtcgttgt cttctaccac
     1141 cacttcctta ttcgaccggc gccagttgtc ttccagcttg cgcgagcccc atttgcgggt
     1201 gaattcgttg atgccgaagg aaaccgtcga agggttgtag aaataccatg cagcacccga
     1261 gccgaggttg ttctggttcg cgaggttgtt attgttgccg acaccggact ggttctgctg
     1321 gctctgaagt tctttcttgc ggtcgtcctc ttctttctgc tttgcaataa gtccgtcgat
     1381 gaacgggtaa agcgcggtcg tgtcggaacc gtacctgttc acgatattct gcaggctgtc
     1441 ttcgttggaa ataatatcca ggaacctgac gagatcgtca aggcttttgc gcttatcggc
     1501 cacgccgtca taatcgggat aatcacgcgg gaggaactgc agcgtgctgt cgtaataggc
     1561 gccggcgcct ttgtaatccg tatcggcgag ataaagatcg ccgatggcga ggtacgaaac
     1621 ggctttctgc cggctgttgc ccacgctggc ggcaaccgaa agcttgtaat atgcaagcgc
     1681 gccggctttg tcgccagatt tttcctcaag ctgcccgagc gtataataga tctggtcctg
     1741 gaactcgatg ttcttttcgt ccgtcagcat tttctgcagc tctttcttgg ctgccgtcct
     1801 gttcttcacg tcgcttgcgc tcaggcgcgc gcggttgagc tttgcattga aaagcatatc
     1861 gtactgcggg tgcagcgcga tgaccttgcc ataggttgca tacgcatctt tctccttgcc
     1921 gactttttca tacagctgcg cgagcacgaa aagataacgg gcgcgggtgc tttttttctt
     1981 cgtgagcagg atcgcctgct cgaggtattt gatcgcattc gggtaattgc ccgtgtgcat
     2041 gtaaaggtcg gcgtatgtgg ctttgacgtc agcgttgagg cggggagggc atgatttatt
     2101 attcgccacc acgtcgagct cggattcggc gtccttataa gcgccgagct gtatctgcgc
     2161 ccgcgctttc cagagcatgc cgtcgaaacg gatcgggaat ttggaatacg tgtcgatgat
     2221 gtaatcgaac atatcaaggg ccgcgaggta ttcacctttg taataatgcg cctgcccgat
     2281 gatgaggtaa ttgtcatcga tccatttgac ggcgcccggg atctcgatgc cgcttttgtt
     2341 ggtgatcgta tgacgggaaa taacgttctg cgacttcttg atcgcttttt cgagatcggt
     2401 gtagcaggct tttgtttcgg gagtgatggg aagcttgaag agcggcagca gctggctgta
     2461 atcgtccacg tacgatttgt ccaccgtgag catggcgctt ttcatggctt ccttcgcgta
     2521 gaaatagcca ttgtagcgcg acgtcatgtt gtgataggaa cgcgtaacga acgtgttctt
     2581 ggtcgtcgag cacgccacga taaaggccat ggtcagcagc aggaaagcga aatggtatga
     2641 tctgaatttt ttcacagact aaaaaggcgg cagcccgaat acgggagggt aacgccggga
     2701 gggcaaaaat agtgtattcc cgtgattaga gacgacggaa atgcgcgcag gttgcccgtg
     2761 gccgggagca accgggcctg attttccgat atttgtgttt ctcgctcgaa aatgcaggaa
     2821 aagaaagggt tcaaacgatt tatggaacgt ctccgcagcc ggtaccggct ggtggtgatg
     2881 aatgacgaga cgttcgagga gcggctttcg gtgcggctga cgccgctcgg cttcctgatc
     2941 ctgatcgcgg cgacgaccat catcatgacg acgctcgtgg tgagcctcgt cgccttcacg
     3001 ccactgcggg agtacattcc cggttacgcc gacgtgggcc tgcagacaaa gctcgattcc
     3061 ctgagcgtgc gtgccgattc cctgcagaat tcgatggttg cgcgaaaaaa atacatggac
     3121 aacctgcgga tcattctcac cgggcaggat tccgcagagc acccgaaaaa tttccgcgat
     3181 tccacgaaaa aatatgcgaa catcaaagcg aaaccttcga aagaagattc tgcactgcgc
     3241 gatgagatcg aagacatgga cgaatattcc ctgaagatgg atgtgcgcca ccagggcatc
     3301 agcgggtttt tctttttcgt gcccgtgaag ggaacgatca ccaacccgtt caatatggcg
     3361 gaagggcatt tcggtgttga cattgtcgct gctgatgaga acgagcccat tcgcgccacg
     3421 ctcgacggaa cagtgatctc cgcagggtat acagtcgacg acggctacgt gatccaggtg
     3481 cagcattccg ataacctcgt ttccatttac aaacactgtg cgtcgctgct gaagaaaacc
     3541 ggcgactatg tcaaggccgg cgacccgatc gcgatcgtcg gaagttcagg agagcagatc
     3601 gcggaaccca acctgcattt cgaattgtgg tacaacggaa caccgatcga cccgcaggag
     3661 aacatggtgt tctgaagagc ggagcggttt acgatcgccg gtttacagct gaaaacagtc
     3721 aggtagcaac attccgtctg cagggctccg ttatctttgt cgcggatgag cgaatccgaa
     3781 acccgaaatc cgaaacctga aatccgccgc ctggccattc tcggcagcac cggctccatc
     3841 ggcacgcagg cgctcgatgt ggttgccgcg catcccgggc atttcgaagt ggaagtgctc
     3901 gccgcgggga ataacgcgga gctgctggtc gcgcaggcga aaaaattccg gccgaacgtc
     3961 gttgtcatca acgatacggc gaagctggcg ttcgtgaaag aagccctgca cgatgacgac
     4021 ataaaagtgt acggcggaga agaagcgctg tgcgacgtgg tgcagatgga aacgatcgac
     4081 ctcgtgctgg ccggcatcgt cggttatgcc gggttgcgtt cgacgctggc tgcggtgaaa
     4141 gccgggaaac agatcgcgct ggcgaacaag gaaacgctcg tggtggccgg ggaaatcgtt
     4201 acggcagaag cgagaaagaa cggcgtaaat ttatatcccg tcgattccga gcattcggcg
     4261 atcttccagt gtcttgccgg ggaattccac aatcccatcg agaaaattta cctcaccgca
     4321 tcgggcggac cttttcgcgg aaagaagcgt gatgagcttt tgcacgtcac caaaaaggat
     4381 gcgctgaaac acccgaactg ggaaatgggc gcgaagatca cgatcgattc ggcgacgctg
     4441 atgaacaaag ggctcgaggt gatcgaagcg aaatggctgt tcggactgaa gccggagcag
     4501 atcgacgtca tcgtccaccc gcaaagcatc attcacagca tcgtgcagtt ctgtgacggc
     4561 agcatgaagg cgcagatggg attacctgat atgaagctgc cgatccagta tgcgctcggt
     4621 tatccgcagc gcctgcgttc ggattttccg cggttcaatt tcatgaacta cccttcgctc
     4681 accttcgaaa aacccgatac ggaaacgttc ccgtgcctgc agctcgccat ggacgcgctg
     4741 aagaaaggcg gcaacatgcc ctgcgtgatg aacgccgcga acgaagtggc tgtgcaggct
     4801 tttctcggag agaaaataag cttcctgcag atcgcggaag ttatttcggg cacgatggcc
     4861 caatcgcatt tcctggcaaa accgtcgtat gaagattatg tgaagagcga tgaagaagcg
     4921 agacgggtgg cggtgcattt tgcaaaataa tccgcgtccc gcgtgatcat cagccggaga
     4981 gtcccgcatc cgcggtctga aaattgtact aactttcttc aaacttagtt ccatgcaaaa
     5041 gatctccgcc cgtttcggca ttctttcagc tgcttttttc ctgttcattc ttcccgcttc
     5101 cgcccagttg aaggtgaagg atgttgacac ggcgtattac aataaagtta tcgcgaccgg
     5161 gaagaaactt tccatgaagc aggatgcgct gctcggtgcg aaagccgccg ccctgccgca
     5221 gaatttcacg gacacgctga aaaaatacga ctggtacgag ctcgacaact attcatttgc
     5281 cgataaggaa taccgcggac ggtttacgga ggaagacgca tccaattccc ggcagtatga
     5341 ttttttccga tacgacgccg acggcacgat caacacgatg tatctttttc ataccatcaa
     5401 gcagggagaa tacagcttct acaccaccac gttcgacaag aacacggcag aaaagctggt
     5461 gggcgtggag aacatcaggg gcgctgcgta catggtctct tctgcgtacg gcgaaaaaga
     5521 atggcagaag atcattagct acaaagacca ggtgatgatc aaagaactga cacgttacgg
     5581 taagcccggc gaaaccccgg tgaaattccg catcgcgtat atggcaatga cgcgggacga
     5641 taaataagga tttttcattg acagggaatt tttccggccg ccgtcctgga acgcagcatg
     5701 cgcgtttcgg ccgcggtata cttttcacct ttttcccgct gctcttttct gttttcgctt
     5761 cagcgcagcc gatcattgcc tgcagaagtg ataatgtttg ttaatggaac accggtcctc
     5821 atttgcccgg cctgggtgaa ggcgctgttt aatgcttatt tttgcgggtt cccggatttt
     5881 cgccggcata aactcgtaaa ccgtacgttg taaatcacac tttcctccgt ggtaaaattc
     5941 accctctttt tcttcagtct ttccttactc atcatactcc acgagttcgg tcatttcatc
     6001 actgcacgct ggttcaaggc gcgcgtggat aaattctatc tcttcttcga ttttcttttt
     6061 ccgttcccgg gcattgccaa atttgcgctg ttcaaaaaga agatcggcga gacggaatac
     6121 ggcatcggct ggttcccgtt cggcggatac gtcgacatcg ccggaatgac ggctgattcg
     6181 gaagaagaca agaacaaaaa agacaaggac gcgaaagagg gcgaagaagg aaaggacgcg
     6241 ccggaagaga aaaaagaacc gaagccgccc gggaagcacg agttccgcgg gaaaaaacca
     6301 taccagcgtc tcatcatcct tgcgggggga attaccgtga acgcgctgct tgccattgtc
     6361 atctacagca tggtgtcgtg ggcgtacggc gaagcgtatc ttcccgtgaa aaacgcgaaa
     6421 tacggaatgg ccgtcgactc cgtagcgatg gggatcgggc tgaaggacgg cgacaagctt
     6481 ttgtctgtcg acaacaagcc tatggaaagt ttccaggatg cgaaagtggc gatcatcctc
     6541 gacaaagcga agacgataca ggtcgaccgc aacggcgaaa aagtgaacat cagtgttccc
     6601 gacggctttg atcagaccgt ggtggaaaaa gatgtcaagg tattgttcga tgcgcgcttt
     6661 cccttcgtgg tggacagcat tattcccggc atgcctgcgg caaaatcggg gatgatgaaa
     6721 ggcgacagca tcgtttccat cgacggcgat acgctcgtgt atttccagca gatcgcgcgc
     6781 aagctttccg ggtatgcgaa caaaacagcg cacgtgggaa ttgtccgcaa cggctcctgg
     6841 aaaacgatcg atatcgcgat caacggggaa ggaaaaatgg gcgtgctgcc ctacgcggaa
     6901 gaccgctaca tgacgctcaa cacgaaagag tacggcttct tcagcgcatg ggggcgcggg
     6961 ttccagcgta cgggcgaagt gctcgtcaac tatgtgaagc agctgcgtct tctcttcacc
     7021 aaagcgggcg caaaaaagat cggcggtttc gccaccatcg gcggactttt cccttccacg
     7081 tgggactggg aagcgttctg gaatatcacg gcattccttt ccgtcattct tgcgttcatg
     7141 aacctgctgc cgatccccgt actcgacggg gggtacattc ttttcgtgct gtgggaaatg
     7201 atcacgggca aaaaagtgag cgacaagttc atgcagaaag cgctcaccat cggcatgtac
     7261 atcgtgctcg gcctgctcat tttcgccaat ggaaacgaca tcctccgggc gttccggcat
     7321 tagttttgca aataaaaaag cggaaagccc gttattcata cacatgaaag ccgctctgcg
     7381 cagcctgttc tgcatacttc ttttacctgc gatcacattc gcgcaggtga atacgcgcaa
     7441 tgacggcaac gacaacccgc cgaaagcgcc caacgggaaa acgaaagtgc tcatcattcc
     7501 gtgggaaccg atcacgttca acgtcagctc cgatgtctcg cgtgcgatca gcaatgaaac
     7561 ggggcagaaa tacgaccaga tcgaagaatc gctgcgcaaa ggattggtcg accagctgaa
     7621 aaaatcattc ggccccacgt gcagcgtcac gtctctcctc gacgatacgg cgaagatgaa
     7681 aaaggatctg cgctatacgt atgtcgttac gcagcgttcc tacacaccgg tgaattttcc
     7741 gctcaatccc acgaaagccg actcggcgaa gctcaacgca cctaagcagg gaacgagcaa
     7801 cgggcagatc cagacaacgg atgatgaaac ggataaattc atgaactgca ccgtgctcag
     7861 cccgaacctg ctcggctacc tgcatgcgaa gtacggggcg gattacatca tcttcatcaa
     7921 cgagatcgac atcgataata atttcggcgc cgatccgtac aacacgcagg gaaaaacgga
     7981 ttacgtgcgc gatgttgtcg tgcactggac catctacagc gccacgactg aaaagcgggt
     8041 ggccgccggc aaaagcaagg gaacattttc ttccgccacg aacacgcctt caaaaatttc
     8101 ttccggggcg ttttcggcga tcgcgaaaga tatttattcg cggtatctcg ccggcatcaa
     8161 gccgaaaaag taaagcgggt ttgccgggca aaatgggcta actttgaaca aacccgttcc
     8221 catgatccgt cctgctttcc acattttttt tactgcgatc gtattcttcg tgacgcttcc
     8281 ttcgcgcagc caggtcgtca tctgcgacac gatgccgctt gcatatgtca gccagggtct
     8341 tcgcttttcc ggtggatttt cctgcgccga ttccgttatc actatcccta ttgtcaacaa
     8401 cacgacgaca ggtttcgcgt acccgctggc gaagatccgg aacaccacgc cgctgccgcc
     8461 cggcatgagc ctgcacagca gcaactggca ggtcttcgcg tcctcctgga atcccggcga
     8521 cacgatgccg gcgtatatct gcttcgatgt aacgcaacca gttccggtca actacacggt
     8581 gacgttcgaa ctttacgttt ccaacttcct tccgctgcag gtcgattcct gtgtgtttgc
     8641 caacgttttc accattaatc ttaacccgct tgcctcagcg ggtgacgggg ttgccatgtc
     8701 cacgggcttt tcattttcgc aggacgcaaa aaacgcattg cttgtgctga aaaataattc
     8761 cggccatgaa atgcacatcc gtattttttc agccgatggc aaaacggtct catcctcttc
     8821 gtgtgcaccg ggatcttctt ccctgagtat tgccggattg ccgccgggaa tttatttcat
     8881 cggcagcgaa gaagaaggct gctgcggaaa gtttatcgtc ccgtgaacgc cgggcgcgaa
     8941 aacggatttt cttaaattag ctactccctg taattagcta ctccctgttt ttttaaatga
     9001 agctgctatc gacttttatc gtgtccgccg tttttttctt cagcgcggat gcacagaatg
     9061 ttcccgtttt cggcaatgag atccccgtga cgatcaccgg gcttacattc gatgcgatgg
     9121 aaccgttcat ttctcccgac ggaaattatc ttttcttcaa cagcctgaac gacggcatca
     9181 cgacaagcct gtattatgcc gtacgtgtga acgattccgt gttcacctac atgggcgcgc
     9241 tcaacggcgc tgaccagacc gtgacgcccc gcctcgacgc cgtcgcttcc tgcgattcgg
     9301 tcaataattt ttactggacg tcgacgcgca actggccggc gcagtacgat aatttcttcc
     9361 acggaacatt taacggcagc gacgtggtga acaccggccg cgtgcatgga aatttttaca
     9421 tctacacgcc gggctggatc gtcatggatg ccgcgatcag cacagacggc agcctgctct
     9481 ggtattgcaa tgcctatttc aacaactgtt cgaacatgcc gtgcaaagcg cagcttggcg
     9541 tcgccgcgaa gctgaacgat tccacgttcg cgaagctggc gaattccgac gggctgctga
     9601 tgaacgtcaa cgacacgaat tacgacgtgt atgcacccaa catttcctcc gacgggctcg
     9661 agttgtattt cacgcgcatc ctgaaaaaca atcccacgca gacgaccatc tgcgtttccg
     9721 tgcgcaatac gttgaatgat acgttcggcc tgccggttgt ggtttacggg tccccgcttc
     9781 tgcccgaagc agccacattg acgaccgaca tgtcgcgtat gtattaccat aaaaaaccgg
     9841 gcagccagta ccagatcttc ctgcgctacc gtacatcgcc gtctgggatt gaagcgacgg
     9901 ataacgcgaa cccgtttttc attgcgccga acccctgcca tgatgtgctg cggatttttt
     9961 ctgccgatgg aacggcgcca ggccgggttg tcatttacga cgttgccggg cagatcgtcc
    10021 ggacggaggc tgacaaaacg accctggtgg atatttccgc tttgcaggcc ggtgtttatt
    10081 ttgtttctgt aatgactgct gaaaaaccgg ctgtcatccg cattattaaa acgtactccg
    10141 gtgaataacg aaatacaaac cggggatttt tacgttgccg ggatttccgt gcgcaccacc
    10201 aatgcaggcg gacaatccat gaaagatatc ggtgaattat ggggaaggtt tttcgccgaa
    10261 aacatttccg caaaaattcc cggaaaagta agcgcggata tttattccat ttacaccgac
    10321 tatgaatccg atcatacggg tctatacacg acgctcctcg ggtgccgtgt aagctcagtg
    10381 gaagatcttc cggaaggaat gaccgggaaa aagattcccg gagggaagtg tatgaagttt
    10441 acgtgcaaag gaaaaattcc ggatgtcatc gctgcgcagt gggcgaacat ctggaacgac
    10501 aaaacgctgc ggcgcagctt cacttcggat tatgaagtgt acaatgaaag agcgcagaac
    10561 cccgcggatg ctgaagtgga tatttttgtc gcggtgaaat aatcgttctc tttcccaaag
    10621 caaaacaggc ccccgttttc cgggagcccg ttgtgcgcat taaccaaacc aacatcacta
    10681 cactactgga ggagagagac gtgaccggta tagcgatgca gtttacccct gaagtcggtc
    10741 acgtctattt tgtacacata aacatcttgt tgcgcaatgt ccgatccccc gttgccgcgg
    10801 ccgtcccatc ctttgttgat gtcgttcgac tcgaagatca tgttgcccca gcggtcgaac
    10861 atcatcatgt ggaaggcgga cacttcattt ccgtaagcga ggaacacatc gttcttcccg
    10921 tcgccgttcg gtgtgaaggc gttcgggatg aagagcgtga attcaggatc gatagcgaca
    10981 ggcatttccg ttgtgtccat gcagccgccg acggatgtcg caatgaggcg cacgttgtac
    11041 gatcctttgt cggcgtaggt gtgtgaagga ctcggcacat tgctcgtgtc attgtcgccg
    11101 aaatcccagt gccacccgat cgtgttcgca gaacattgat tctggaaaag gatcgtgggc
    11161 tcgagctcgc tgacgtcatt cgctgacggg ctgaacccgg cgtgcggcga ctggtaaacg
    11221 attgcgctgg ccgtcgtcgt tgtgctgcct gtgcaaccaa agggtgaaat aatatgcacg
    11281 gttactaaat agttgccgtc atccgtataa tcgtggtacg tgctgctgcc ggcggcggtg
    11341 tacccatctc cgaaatccca cgaataggtg cagccggcat tggcgggatc attgtctgcg
    11401 aactggaggc gaacctggtc gcagccggac ccggattgcg gggacagcgt aacaacggga
    11461 ttcgggtgta ccgtgatcgg gatcgattga gagacgacag tgccgcagat gttcgttacg
    11521 ttcacctggt acgtagtgct cgaagaaggt tgtactgcga acggtccgcc ggtggtccag
    11581 ggctggttgt tccagttcca cgtaacgggc ccggtgttcc cgttgagcat cgcgctgatg
    11641 gtgacctgtg tgccctggca gacgcttgca ggtcccgaaa ccgtgagatc gccgggaacc
    11701 agcgagaaca tatcgacggt gacgctcgct gacggacttg tgcagccgtt ggcatctact
    11761 gcatgaacag tgtacgtcgt cggtgccggc gggtaaaccg tgtagctgga attcggtccg
    11821 agtgccgggc tccacgaata tgtgtagctg ccggttccgc cttgcgcatt tgcgccgatg
    11881 ggagccggct gcccggaaca gatggtg
//
LOCUS       DASTSF010000044        17585 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1081540,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000044 DASTSF010000000
VERSION     DASTSF010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 17585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17585
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1081540"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..662)
                     /locus_tag="VFU15_01305"
     CDS             complement(<1..662)
                     /locus_tag="VFU15_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009860735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HEU4716436.1"
                     /translation="MIIKSKAPLRLGLAGGGTDVSPYSDLYGGAILNATISSYAYAVL
                     EPMENGKIELVSGSPENVFIHHSADELPIDNRAYELQCGVYNHIVKHYTKKPLSFRMT
                     TWIDAPQGSGLGTSSTLVVAILGAFTEWLKLPLGQYDIAHLAYEIERIEMGMAGGKQD
                     QYAATFGGVNYLEFFANDKVIVNPLSIKPDVLNELEMNLLLYFTETRRESAQIISHQV
                     ENV"
     gene            complement(701..1852)
                     /locus_tag="VFU15_01310"
     CDS             complement(701..1852)
                     /locus_tag="VFU15_01310"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU4716437.1"
                     /translation="MERKKIFILGPAFPLRGGLATFDELLCRSLNEAGHDTRIISFSL
                     QYPSFLFPGTTQYDKSGKAPAGIKIETRINSVNPFNWLKTGRYIRKERPDFLIFRYWL
                     PFMGPALGTIARRAKKNGHTKILAIVDNAIPHEKRPGDRMFSAYFLKSCDGYLTMSHK
                     VMDDLSQFTKSELKLFTEHPLYTSFGEAEDKKKAREFLGIEPDGKYLLFFGLIRRYKG
                     LDLLLEAMSDERVKATGAKLIVAGEYYEDSSYYDEIIQRFNLSRNVILHTKFIPHEEV
                     RHYFSACDLVAQTYHTATQSGVTKIALHFDKPSLVTDVGGLGEIILHGKSGYVVPPKA
                     KDIADSIVDFYSNHREKDFVNATIEEKKKYGWDVMVDSIETLYRRVIES"
     gene            1952..2146
                     /gene="rpsU"
                     /locus_tag="VFU15_01315"
     CDS             1952..2146
                     /gene="rpsU"
                     /locus_tag="VFU15_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEU4716438.1"
                     /translation="MIVVPVKEGESLEKALKKFKKKFEKTGVVRELRDRQKFTKPSVR
                     KREVKLKAIYKQHMQLDVEA"
     gene            2243..3157
                     /locus_tag="VFU15_01320"
     CDS             2243..3157
                     /locus_tag="VFU15_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014678777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEU4716439.1"
                     /translation="MNEHPFNSFIDFIRNEKRYSPHTALAYESDLIQFRDFLVETYGD
                     MAPAEAGYQVIRSWIARLMDQGMDPRSVNRKITSLRGYYRFLRVSGEITSNPTHRIQG
                     PKISKRLPAFVEERNIDNLLDSNILKESPGDEYSRMLDRLIIEMLYATGIRVSELVNL
                     AGNNINYSKSTIKVLGKRNRERIIPVPDELLELITVFRRFRDNELAKNGLSAGSELLV
                     DSRGKKLSRGFVYKKVRYYLSLVTTIEKKSPHVLRHTFATHMLNNGADLNAIREILGH
                     TSLAATQVYTHNTVEKLKAVYHKAHPRA"
     gene            3190..3489
                     /gene="raiA"
                     /locus_tag="VFU15_01325"
     CDS             3190..3489
                     /gene="raiA"
                     /locus_tag="VFU15_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017843475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEU4716440.1"
                     /translation="MEIQIQSIHFTADHKLLSFIEDKVGKLSHFYDGIISSEIFLRLD
                     KSDSTTNKVAEIKINVPGNDLYVKRQCKTFEEAIDTGVTALSRQVKKYKDKLKGI"
     gene            3711..3786
                     /locus_tag="VFU15_01330"
     tRNA            3711..3786
                     /locus_tag="VFU15_01330"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3744..3746,aa:Thr,seq:tgt)
     gene            3838..3919
                     /locus_tag="VFU15_01335"
     tRNA            3838..3919
                     /locus_tag="VFU15_01335"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3872..3874,aa:Tyr,seq:gta)
     gene            4007..4079
                     /locus_tag="VFU15_01340"
     tRNA            4007..4079
                     /locus_tag="VFU15_01340"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4040..4042,aa:Gly,seq:tcc)
     gene            4137..4208
                     /locus_tag="VFU15_01345"
     tRNA            4137..4208
                     /locus_tag="VFU15_01345"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4169..4171,aa:Thr,seq:ggt)
     gene            4323..5510
                     /gene="tuf"
                     /locus_tag="VFU15_01350"
     CDS             4323..5510
                     /gene="tuf"
                     /locus_tag="VFU15_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018696712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEU4716441.1"
                     /translation="MAKEKFDRSKPHVNVGTIGHVDHGKTTLTAAITAVLADKGLAQA
                     RDFSSIDNAPEEKERGITINTSHVEYETANRHYAHVDCPGHADYVKNMVTGAAQMDGA
                     ILVVAATDGPMPQTREHILLARQVGVPKIVVFMNKVDMVDDPELLDLVEMEIRELLSF
                     YQFDGSNTPIIRGSALGGLNKEPKWVEKIMELMDAVDSYIPTPARDKEKPFLMSVEDV
                     FTITGRGTVATGRIETGVVNVGEEVEIIGMQEEKLKTTVTGVEMFRKLLDRGEAGDNV
                     GLLLRGIEKKDIRRGMVIAKPGSITPHTEFKAEVYVLKKEEGGRHTPFHNKYRPQFYF
                     RTTDVTGEIELPAGREMVMPGDNVTISVKLIVPIAMDKGLRFAIREGGRTVGAGQVIE
                     IVK"
     gene            5611..5681
                     /locus_tag="VFU15_01355"
     tRNA            5611..5681
                     /locus_tag="VFU15_01355"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5643..5645,aa:Trp,seq:cca)
     gene            5697..5897
                     /gene="secE"
                     /locus_tag="VFU15_01360"
     CDS             5697..5897
                     /gene="secE"
                     /locus_tag="VFU15_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018696711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEU4716442.1"
                     /translation="MFNKIRAYYDEVRDELVNKVTWPTWSELQESAVIVMIATVIFAV
                     IVFAMDFSFNKGMEAIYKFIGG"
     gene            5929..6495
                     /gene="nusG"
                     /locus_tag="VFU15_01365"
     CDS             5929..6495
                     /gene="nusG"
                     /locus_tag="VFU15_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010417766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEU4716443.1"
                     /translation="MAEQIQHKEQQVKKWYVLRVVNGKEKKIKQYIESEISRLGMQDY
                     VSQVLIPIEKVIQIRNGKKVSKERNIFPGYVMLEGSLVGEIPHVLKNITGVIGFLGSK
                     TGDPVPLRPSEINRILGKVDELAGAEETMLNQFVVGETVKVIDGPFNTFSGVIEEINE
                     EKKKLKVMVKIFGRKTPLELNYMQVEKE"
     gene            6560..7006
                     /gene="rplK"
                     /locus_tag="VFU15_01370"
     CDS             6560..7006
                     /gene="rplK"
                     /locus_tag="VFU15_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009085663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEU4716444.1"
                     /translation="MAKEVSALIKLQIKGGAANPSPPIGPALGAKGVNIMEFCKQFNA
                     RTQDKAGKVLPVIITVYNDKSFEFIIKQPPVAVQLKDAAKVQTGSGESNRKKVGSVSW
                     DQVRTIAESKMPDLNCFTIESAMSMVAGTARSLGINVTGTNPLKSN"
     gene            7070..7762
                     /gene="rplA"
                     /locus_tag="VFU15_01375"
     CDS             7070..7762
                     /gene="rplA"
                     /locus_tag="VFU15_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002673670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEU4716445.1"
                     /translation="MANLTKKRKAAIAKYDPAKSYALQDAAKIVKDITTTKFDASVDL
                     SIRLGVDPKKANQMVRGTVTLPHGTGKTIRILVICTPDKEAEAKAAGADYVGSDEFIQ
                     KIKDGWTDVDVIITTPSMMGKVGALGKVLGPRGLMPNPKTGTVTMEVGKAVKESKGGK
                     IDFKVDKAGIIHAAVGKVSFEAAKLADNANEILQAIIKMKPASAKGTYMKSVYLSSTM
                     SPSVQVETKTLA"
     gene            7798..8322
                     /gene="rplJ"
                     /locus_tag="VFU15_01380"
     CDS             7798..8322
                     /gene="rplJ"
                     /locus_tag="VFU15_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006987981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEU4716446.1"
                     /translation="MNKENKTQIINDLAELLANNKNFYLADTSELPSDKTSSLRRKCF
                     DKKIKLLVVKNTLLRKAMERANDGSFNPLYVTLKGSTSIMFCESGAEPAKLIKEFRKT
                     NSKPVLKAAFVEQSVYIGDDQLDSLASIKSKNELIGDIIGLLQSPAKNVISALQSSGG
                     KIAGVVKTLSERPE"
     gene            8409..8786
                     /gene="rplL"
                     /locus_tag="VFU15_01385"
     CDS             8409..8786
                     /gene="rplL"
                     /locus_tag="VFU15_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009642430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEU4716447.1"
                     /translation="MADVKALADQLVNLTVKEVNELAKILKDEYGIEPAAAAVAVAAA
                     PGAGGGAAAAEKTTFDVVLKNGGANKLQVVKVVKDVTGLGLKEAKDLVDGAPKPVKEG
                     ISKEDAEALKKQLTEAGAEVEVK"
     gene            8920..12729
                     /gene="rpoB"
                     /locus_tag="VFU15_01390"
     CDS             8920..12729
                     /gene="rpoB"
                     /locus_tag="VFU15_01390"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008613329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEU4716448.1"
                     /translation="METTKKQQRINFAKSKAQIDYPDFLEIQLKSFQDFFQLETTSEN
                     RQNEGLFKVFSENFPISDARNNFVLEFLDYFVDPPRYSIDECIERGLTYSVPLKAKLK
                     LYCTDPEHEDFETIVQDVYLGTIPYMTPRATFVINGAERVIVSQLHRSPGVFFGQSRH
                     ANGTKLYSARVIPFKGSWIEFATDINNVMYAYIDRKKKLPVTTLLRAIGWESDKQILE
                     LFGLADEVKVSKAGLKAVVGRKLAARVLKTWVEDFVDEDTGEVVSIERNEVIIDRETI
                     LDNNHIDLIVDANVKSIILHKEDAANLDYSIIYNTLQKDTSNSEKEAVEHIYRQLRNA
                     EPPDEETARGIIDKLFFSDKRYDLGEVGRYRLNKKLKLNTAEDVRVLTKDDIIAIIKY
                     LIELINSKADIDDIDHLSNRRVRTVGEQLYSQFGVGLARMARTIRERMNVRDNEVFTP
                     TDLINAKTLSSVINSFFGTNQLSQFMDQTNPLAEITHKRRLSALGPGGLSRERAGFEV
                     RDVHYTHYGRLCTIETPEGPNIGLISSLCVYAKINKLGFIETPYRTVTNGKVDVNKHV
                     VYLTAEEEDQKIIAQANVDVNDKGQFASNRVKARYESDYPVVEPEQISLMDVAPNQIA
                     SIAASLIPFLEHDDANRALMGSNMQRQAVPLLRPQAPIVGTGLEGLIARDSRVLWQAE
                     GDGVVEYVDSTEITIRYNRSDKDRLISFDDDVKSYRLTKFRKTNQSTCINIRPMVKKG
                     DKVKRGQILCEGYATKDSEIALGRNLKVAFMPWKGYNFEDAIVISERVVRDDIFTSIH
                     IDEYVMEVRDTKRGLEELTADIPNVSEEATKDLDENGLIRVGAEVKEGDILIGKITPK
                     GETDPSPEEKLLRAIFGDKAGDVKDASLKVPPSIRGVVIDKKLFSRAIKDKKAKAEEK
                     TLLEKIEKEEAVESAALRDTLIQKLSSILNGKTSAGVFNNLKEEVVEKGTKFSGKVLE
                     KIDFNNVNPARWTNDNDTNELVKITLHNFGIKFNDLGGVYKRKKFALQVGDELPAGIV
                     QLAKVYIAKKRKLKVGDKMAGRHGNKGIVARIVRDEDMPFLEDGTPVDIVLNPLGVPS
                     RMNLGQIYETVLGWAGQKLGMKFATPIFDGATIDEINKYTDEAGIPRYGRTYLYDGGT
                     GERFDQPATVGIIYMLKLGHLVDDKMHARSIGPYSLITQQPLGGKAQFGGQRFGEMEV
                     WALEAFGAANILQEILTIKSDDVVGRAKAYEAIVKGENMPTPGIPESFNVLLHELRGL
                     ALNVNLD"
     gene            12770..17062
                     /gene="rpoC"
                     /locus_tag="VFU15_01395"
     CDS             12770..17062
                     /gene="rpoC"
                     /locus_tag="VFU15_01395"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016197087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEU4716449.1"
                     /translation="MSFRKEFKIKSNFSTITISLASPESILERSSGEVLKPETINYRT
                     YKPERDGLFCERIFGPVKDWECHCGKYKRIRYKGIVCDRCGVEVTEKKVRRERMGHIN
                     LTVPVAHIWYFRSLPNKIGYLLGLPTKKLDMIIYYERYVVINAGVKAADGINYMDFLT
                     EEQYLEILDTLPKENQYLDDNDPNKFIAKMGAEALYDLLQRIKLDELSYELRHKANTE
                     TSQQRKNEALKRLQVVEAFRHANKNVENHPEWMIIKVVPVIPPELRPLVPLDGGRFAT
                     SDLNDLYRRVIIRNNRLKRLIEIKAPDVILRNEKRMLQEAVDSLFDNSRKSNAVKTES
                     NRALKSLSDSLKGKQGRFRQNLLGKRVDYSARSVIVVGPELKLHECGLPKEMAAELFK
                     PFIIRKLIERGIVKTVKSAKKIVDRKDAVVWDILENVLKGHPVLLNRAPTLHRLGIQA
                     FQPKLIEGKAIQLHPLVCTAFNADFDGDQMAVHVPLGNAAILEAQILMLASHNILNPA
                     NGAPIAVPSQDMVLGLYYMTKGMKTDDKITVKGQGLTFYNAEEAIIAYNEGRVHLNAF
                     VKVKVNNAREEGKLVTKVIETTLGRILFNQVVPAEVGYINELLTKKSLRDIIGNVLKE
                     TGMAKTSQFLDDIKELGFRMAFKGGLSFNLGDIIVPEEKEKMIAEANEQVEEVKSNYN
                     MGFITNNERYNQIIDIWTHTNNRITKRVLDQLSSDRQGFNPVYMMLDSGARGSKEQIK
                     QLGGMRGLMAKPSKAGSAGGEIIENPVISNFKEGLSILEYFISTHGARKGLADTALKT
                     ADAGYLTRRLVDVAQDVIITEEDCGTLRGLVATPLKKNEEIVEPLHDRILGRTTVHDV
                     YDPQTGELIIESGNEITEEYASRIDQTAIESLEIRSVLTCESKQGVCAKCYGRNLATG
                     RMVQQGESVGVIAAQSIGEPGTQLTLRTFHVGGTASGTATASKLEAKYDGIIEIEELR
                     TVKRKTENGPVDVVIGRSAEMRIIDTNTQIVLTTGNIPYGSQLNVKNGETVKKGQTIC
                     EWDPYNAVIVSEFGGKLEFEHIEEGITFREEADEQTGFREKVIIESKDKTKNPSIRIV
                     SGSDILKTYNIPVSAHIVVDDGAKIEAGQILVKIPRALGKSGDITGGLPRVTELFEAR
                     NPSNPAVVSEIDGVVTFGKIKRGNREVHVESRTGERKTYLVALSKHILVQENDFIRAG
                     EALSDGSISPGDILAIKGPTAVQEYLVNEVQEVYRLQGVKINDKHFEVIVRQMMRKVE
                     IVDPGDTMFLEQQIANKTDFMEENDRLFGKKVVVDSGDSSSMKSGQIISARKLRDENS
                     MLKRKDMKQVEARDAVPATSSQILQGITRASLQTNSWVSAASFQETTKVLNEAAVNGK
                     QDHLLGLKENVIVGHLIPAGTGNRAYEKLVVGSKDEYEKLMAAKKEAMEEEV"
     gene            17133..17522
                     /locus_tag="VFU15_01400"
     CDS             17133..17522
                     /locus_tag="VFU15_01400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716450.1"
                     /translation="METVKTAGREDLGFALYERREDGVIVLHLENDLMIDLEKAERMN
                     EALGRITAGIPSKILVLTGKYSSADKEARQLLSSGQKRLQIAKAGVVIHSLSQKLLAN
                     FFSNVHKPPFPVRFFNSAEDAEKWLRD"
BASE COUNT         4754 a         4438 c         4540 g         3853 t
ORIGIN      
        1 acattttcca cctggtggct gatgatctgc gccgactctc tccgggtttc cgtgaaatag
       61 agcagcaggt tcatctcgag ttcattcagg acgtccggct tgatcgaaag cgggttgacg
      121 atcactttgt cattcgcgaa aaattcgagg taattcacgc cgccgaacgt agctgcatac
      181 tggtcctgct tcccgccggc cattcccatt tcaatgcgct cgatctcgta ggcgagatgc
      241 gcgatgtcgt actgaccgag cggaagcttc agccattcgg tgaatgcgcc gaggatggcg
      301 accacaagcg tggacgaagt gcccagcccc gatccctgcg gcgcatcgat ccaggtggtc
      361 atccggaacg aaagcggctt tttcgtgtaa tgttttacaa tgtggttgta tacgccgcac
      421 tgcaattcat acgcgcggtt atcaatcggc agttcgtcgg ccgaatgatg gatgaataca
      481 ttttccgggg agcccgaaac cagttcgatc ttcccgtttt ccattggctc cagcacggca
      541 tacgcgtaac tggagatcgt cgcgttgaga atggccccgc cataaaggtc cgaatacgga
      601 gaaacgtctg ttccgccgcc ggccagaccc aatcgcaacg gtgctttgct tttaatgatc
      661 ataacggata taaagctgcg aaaatccgcg gaatccgcgg ctaactttca attaccctcc
      721 ggtaaagtgt ttcgatgctg tccaccatca cgtcccatcc gtatttcttt ttttcttcaa
      781 tggtggcgtt cacaaagtct ttttccctgt gattggaata aaaatccacg atgctgtcgg
      841 cgatgtcttt tgccttcggc ggcaccacgt atcccgattt cccgtgcagg atgatctcgc
      901 ccagtccgcc gacatccgtt accagcgagg gtttgtcgaa atgaagcgcg attttcgtta
      961 caccgctttg cgtggcggtg tgataggtct gcgccacgag atcgcacgcg gaaaaatagt
     1021 ggcggacctc ttcgtgcggg atgaatttcg tgtgcagaat gacgttacgg gaaagattga
     1081 accgctggat gatctcgtcg taatagctgc tgtcctcgta atattctccg gcgacgataa
     1141 gctttgcgcc ggttgctttc acgcgttcgt cactcatggc ttccagcagg aggtcgaggc
     1201 ctttgtagcg cctgatcagc ccgaaaaaaa gcaggtattt cccgtcgggt tcaataccga
     1261 gaaattccct cgcttttttc ttgtcctccg cttctccgaa ggaagtgtac agcggatgct
     1321 cggtgaacag tttcagctcc gatttcgtga actgcgaaag atcatccatc accttgtggg
     1381 acatcgtcag gtagccgtcg cagcttttca ggaaataagc cgagaacatt ctgtcgcccg
     1441 gccgtttttc atgcgggatc gcattatcga cgatggccag gatcttcgtg tgaccgtttt
     1501 tctttgcacg gcgtgcaatc gttcccaaag cgggccccat gaacgggagc cagtaccgga
     1561 aaatgagaaa atcgggccgc tctttccgga tgtatcgccc ggtcttcagc cagttgaacg
     1621 ggtttacgga gttgatccgc gtttcgattt ttatgcctgc aggcgctttc ccgcttttgt
     1681 cgtattgcgt ggtgcccggg aacaggaagg aagggtattg caatgaaaat gagatgattc
     1741 gggtatcatg acccgcttca ttgagggagc ggcagagcag ctcgtcaaaa gtggcaagcc
     1801 cgccgcgaag cggaaatgcc gggcccagga taaagatctt tttgcgctcc aagtgtggtc
     1861 aaaattaaag gttcagcgac aaatagttgc ataatcacat tggcttttct acatttgcac
     1921 cccttataaa aacagaaaga ggaataaaaa catgatcgtt gtaccggtaa aagaaggcga
     1981 atcgctcgaa aaagcgctca agaaattcaa aaagaaattc gaaaaaacgg gcgttgtccg
     2041 tgagctccgc gatcgccaga aattcacgaa gccttccgtg cgcaagcgcg aggtgaagct
     2101 gaaagcgatt tataagcagc acatgcagct cgacgtggaa gcgtaagcgt tcccgttcac
     2161 aattttttac tgaaatacac acaattggac aaacttcgaa gtgggtttgt cgtacaattg
     2221 tgtgtatttc ttttttccgc gcgtgaacga acatcccttc aatagtttta tcgactttat
     2281 ccggaacgaa aaacgctatt ctccgcacac agcgctggcg tacgaatccg atctcatcca
     2341 gttccgcgat ttcctggtcg aaacgtacgg ggatatggcc ccggcagaag ccggttacca
     2401 ggtcatacgt tcatggatcg cccggctgat ggaccaggga atggatccgc ggtccgttaa
     2461 ccggaaaatc acaagcctca ggggatatta ccgctttctc agggtttccg gcgagatcac
     2521 ttccaatccc acccatcgca tccagggacc gaagatttcc aaacggcttc ccgcttttgt
     2581 ggaagaacgg aatattgata acctgctgga ttccaatatt ctgaaagaaa gtccgggaga
     2641 tgaatattcc cgtatgcttg atcgcctgat catcgaaatg ctttatgcga ccgggattcg
     2701 cgtttcggag ctggtcaacc tcgccgggaa taacataaat tactcaaaat caacgataaa
     2761 ggtacttggc aaacgcaacc gggaaaggat cattccggtt ccggacgaat tgcttgagct
     2821 catcaccgtt ttccgcaggt ttcgtgataa tgaactggct aaaaacggcc tttcggccgg
     2881 aagtgaatta ctggttgatt ccagaggaaa aaaactaagt cgcggatttg tttacaaaaa
     2941 ggtgaggtat taccttagtc ttgttacgac gatagagaaa aaaagccctc acgttcttcg
     3001 gcatactttt gcaacacaca tgctcaataa tggcgcggat ctcaatgcga tccgcgaaat
     3061 tctcgggcat acaagccttg cggcaacgca ggtttacacg cataacacgg tcgaaaagct
     3121 gaaggcggtt tatcataagg cgcatccgcg tgcatgaatc aataacaaca tccaaacaaa
     3181 ggaggaatta tggaaattca gattcaatcc atccacttta cggccgatca caagctgctg
     3241 tctttcattg aagacaaggt gggcaaactg tcgcattttt atgacgggat catcagcagt
     3301 gaaatatttc ttcgcctcga caagtcggat tcgacgacca acaaagtggc ggagataaag
     3361 atcaacgtcc cgggaaatga tctttatgtg aaaagacagt gcaaaacctt tgaagaggcc
     3421 attgacacgg gggtaaccgc gctgtccagg caggtcaaaa aatacaagga taagctcaag
     3481 ggaatttgag cccttcccag gttatgtgaa cagccggccc gaaaggccgg tttttttgtg
     3541 ttttttgggc gccaaaataa atttcaccgt ttgtcggatg tgaaaagttt tatacatttg
     3601 cagtcctttt tgaaaagggc acaggtgaaa tacgcctgaa aagttctttg aaaacagcag
     3661 ttgaacgcaa tgggccgttg gattcttccg gccggatatg accagtagtt gccggtgtag
     3721 ctcagctggt agagcagctg atttgtaatc agcaggtcgg gggttcgagt cccttcgccg
     3781 gctccattca atctctggtg ccggcggtga tcactccggg ccttttagca cctttccggg
     3841 gagatactca agtggccaac gaggacagac tgtaaatctg ttgtcttaga cttcacaggt
     3901 tcgaatcctg ttctccccac atcgtccggc gaagcctcag cgaaaacgga caattcctgc
     3961 ggtcattgaa aatgcaggaa aagcagggca ctgaaaggta aattaagcgg aagtagctca
     4021 tttggtagag catcagcctt ccaagctgag ggtggccggt tcgagcccgg tcttccgctc
     4081 gaaagttgga aatggaaccg atggttccgc ggaactgaaa aagcaactgc tgttcagccg
     4141 ctgtagctca gtggtagagc acttccttgg taaggaagag gtcgcgggtt caattcccgc
     4201 catcggctca ggaaaatgaa atcaagggaa ttcggtaacc ccgacaaaat aatatccgaa
     4261 caaaagagta aacgtttcga aacattaaac agtaacatca acacaattaa aaaaccataa
     4321 ttatggcaaa agagaaattc gaccgttcca aaccgcacgt gaacgtcgga acaatcggtc
     4381 acgttgacca cggtaaaacg acccttacgg ctgcaattac cgctgttctt gcagataaag
     4441 gtctggcgca ggctcgcgac ttctcgtcaa tcgacaatgc acctgaagaa aaagaacgcg
     4501 gtatcaccat caacacctcg cacgtagagt acgaaactgc aaatcgtcac tatgctcacg
     4561 ttgactgccc gggtcacgct gactacgtca agaacatggt taccggagct gcgcagatgg
     4621 acggtgctat tctcgttgtg gccgcaacgg atggccccat gccccagacg cgcgagcaca
     4681 ttcttctggc ccgccaggtt ggtgttccga aaatcgtcgt gttcatgaac aaagtggaca
     4741 tggtcgatga tcctgaactt ctggacctcg tggaaatgga aatccgcgaa ctcctgagct
     4801 tctaccagtt tgacggaagc aataccccga tcattcgcgg ttctgcgctc ggcggactca
     4861 acaaagagcc gaagtgggtt gaaaagatca tggaactcat ggatgcggtt gattcataca
     4921 tcccgactcc tgcccgtgat aaggaaaaac ccttcctcat gtctgttgag gacgtgttca
     4981 ccatcaccgg tcgcggtacc gttgcaacgg gccgtatcga aactggcgtt gtgaacgtcg
     5041 gcgaagaagt agagatcatc ggtatgcagg aagagaaact caaaacgacc gttaccggcg
     5101 ttgagatgtt ccgcaaactc cttgatcgcg gtgaagcagg cgacaacgtt ggtcttctcc
     5161 tccgtggtat cgagaaaaaa gatatccgcc gtggaatggt tatcgccaag cccggatcca
     5221 ttacgccgca cacggaattc aaagcagaag tttacgttct gaaaaaagaa gaaggtggtc
     5281 gtcatacccc cttccacaat aaataccgcc cgcagttcta cttccgtaca acggatgtaa
     5341 ccggggagat cgaacttcct gccggccgcg aaatggtaat gcccggcgat aacgtgacga
     5401 tcagcgtgaa gcttatcgtt cccatcgcta tggacaaagg tcttcgcttc gctatccgcg
     5461 aaggtggccg taccgttggt gccggacagg ttatcgagat cgtgaagtaa ttccggcggt
     5521 ttaagatcaa ctgcagaccg aaggtgtttt tcgccgataa ggcgaagaac acctttcgct
     5581 gccaaaagaa caaactgctg aaacggttaa acaggcatag ttcaatggta gaatagcggt
     5641 ctccaaaacc gttgatcttg gttcgaatcc aggtgcctgt gcaaaaaaca aagagcatgt
     5701 tcaataaaat cagggcatat tacgacgagg tcagggatga actcgtgaac aaggtgacct
     5761 ggcctacctg gtctgaactc caggaaagtg ccgttatcgt aatgatcgcg accgtcattt
     5821 ttgccgtgat cgtttttgcg atggatttct ctttcaataa ggggatggaa gcgatctata
     5881 aatttatcgg aggataaatt ttttcaaacc caatttagct gaattaaaat ggctgaacag
     5941 atccaacata aggaacaaca ggtgaagaag tggtatgtat tgcgcgtggt gaacgggaag
     6001 gagaagaaga tcaagcaata cattgagtcc gagatctcgc gtctgggtat gcaggattat
     6061 gtttcacagg tgctcatccc gatcgaaaaa gtgatccaga tccggaatgg gaaaaaggtc
     6121 agcaaggaaa gaaatatttt ccccggctac gtgatgctgg aaggctcgct ggtcggtgaa
     6181 attccgcacg ttcttaaaaa tattaccggt gtaattggtt tcctcgggtc gaagaccggc
     6241 gaccccgttc cgctgcgccc gtccgagatc aaccgcatcc tcggaaaagt tgatgagctc
     6301 gcaggagcgg aagagacaat gctgaaccag ttcgttgtgg gtgagacggt gaaagtgatc
     6361 gacggaccgt tcaatacctt ctcgggtgtg atcgaagaga tcaacgaaga gaagaagaag
     6421 ctcaaggtga tggtcaagat tttcggccgc aaaacaccgc tcgagcttaa ttacatgcag
     6481 gttgaaaaag aataacctgc tattgatacg ctgttacgcg cttccaacat aacagaaaca
     6541 aaacagtaaa aactgcaaaa tggcgaaaga agtaagtgct ctgatcaaac tccagatcaa
     6601 aggtggcgca gccaacccgt ctccgcctat cggacccgca ctgggtgcga aaggtgtgaa
     6661 catcatggag ttttgcaagc agttcaacgc ccgtacgcag gacaaagccg gtaaagtgct
     6721 cccggtgatc atcacggttt ataatgataa gtcattcgaa tttatcatca agcaaccgcc
     6781 ggtcgccgtg cagcttaaag acgcggctaa ggtccagaca ggttccggtg aatccaaccg
     6841 taagaaagtg ggttcagtaa gctgggacca ggtacgcacg attgccgagt cgaaaatgcc
     6901 cgatctcaat tgctttacaa tagaatcagc aatgagcatg gtggcaggaa cggcgcgcag
     6961 tcttggtatt aacgttacgg gtacgaatcc gcttaaatcc aattgagttt cacctgacgg
     7021 agccccgcgc ttttagcaaa gggcgtttga acgtcgcaaa atcaaaacaa tggccaatct
     7081 gaccaaaaag aggaaagcag ctattgcaaa gtatgacccg gcaaaatcat acgcgttgca
     7141 ggatgcagcc aaaattgtca aggacatcac caccaccaag ttcgacgcat ctgtcgatct
     7201 cagcattcgt ctcggtgtag acccgaagaa agccaaccag atggttcgcg gaaccgttac
     7261 gcttccgcac ggaactggta aaacgattcg cattctcgtg atctgcacac ccgacaaaga
     7321 agcggaagcc aaagcggccg gcgctgatta tgttggttct gacgaattca tccagaagat
     7381 caaggacggc tggacagacg tggatgtcat catcaccaca cccagcatga tgggaaaagt
     7441 cggtgcgctt ggaaaagtgc ttggtccccg cggacttatg ccgaacccga aaaccggtac
     7501 agttaccatg gaagtaggga aagcggtaaa agaatccaaa ggcggcaaga tcgatttcaa
     7561 ggtggacaag gctggcatca ttcacgcagc tgtgggaaaa gtttcttttg aagcggcaaa
     7621 acttgcggat aacgccaacg agattctcca ggcgatcatc aagatgaagc cggcttctgc
     7681 aaaaggaacc tacatgaaaa gtgtttacct cagcagcacg atgagtccga gcgtacaggt
     7741 agaaaccaaa acgcttgcat aacgctccat tgatagcgga gcctaacgca tcacccaatg
     7801 aacaaggaaa ataaaacaca aattatcaac gacctggcgg aacttcttgc caacaacaag
     7861 aatttctacc tcgccgacac ttccgagctt ccctctgaca agacaagctc gctgaggaga
     7921 aagtgttttg acaaaaagat caaactgctc gtggtaaaga acacgctgct tcgtaaagca
     7981 atggaacgtg ccaatgacgg ctccttcaat ccgctttacg ttacgctgaa aggttctacc
     8041 tcgatcatgt tctgcgaatc cggcgctgaa cccgccaagc ttatcaagga attccgtaag
     8101 acgaattcca agccggttct gaaagcggca ttcgttgaac aaagtgtata cataggcgat
     8161 gatcagctcg attcactcgc atcgatcaag tccaagaacg aacttatcgg cgatattatc
     8221 ggcctgctcc agtctcctgc gaaaaacgtc atctctgctc tccagagcag cggtggaaaa
     8281 atcgctggtg ttgtcaaaac gctctctgaa cgtcccgaat aacggacgct gtttaaaagg
     8341 aaaccaatta ctaattcaaa caatcaagtc taatctctcc cgcgttgcgg gaccaaaaac
     8401 aaataaaaat ggcagacgta aaagcccttg ctgatcagct cgtaaatctt actgtaaagg
     8461 aagttaatga gcttgccaaa atcctgaaag acgaatatgg tatcgagccc gcagctgctg
     8521 ctgtagctgt tgccgctgct cccggagcag gcggtggcgc tgctgctgct gagaaaacaa
     8581 cgttcgacgt tgttctcaag aacggcggtg ctaacaagct ccaggtggtt aaggtcgtga
     8641 aagacgtaac cggcctcgga ctcaaagaag ccaaagatct cgttgacggt gcaccgaaac
     8701 cggtgaaaga aggtatcagc aaagaagatg ctgaagctct taaaaagcaa ctcacggaag
     8761 caggcgctga agttgaggtt aagtaatacc gttcttcaga ctgctgaatt tcagtcttag
     8821 acctgtttgc cccgttccgg aacaccggaa cgggggcagg tctaaaccct tttgttgctt
     8881 atcgtgcttt tgtttcactt cttataattc ctctcggcct tggaaacgac caaaaaacag
     8941 caacgcatta actttgcgaa aagcaaagcc cagattgatt acccggattt cctcgagatc
     9001 caattgaaat ccttccagga tttcttccag ctggaaacca ccagcgaaaa ccgccagaac
     9061 gaaggccttt tcaaagtatt cagcgaaaac ttcccgatct ctgatgcacg aaacaatttc
     9121 gttctcgagt ttctcgatta tttcgttgat cccccgcgtt actctatcga tgaatgcatc
     9181 gagcgcggac tcacctacag cgttccgctg aaggcaaaac tgaaattgta ttgcaccgat
     9241 cccgagcacg aagattttga aaccatcgtg caggatgtgt acctcggaac tattccttac
     9301 atgacgccgc gcgctacgtt cgttatcaac ggagccgagc gcgtcatcgt ttcccagctt
     9361 caccgttcac cgggcgtttt cttcggacaa agccgccacg ccaacggaac caaactgtat
     9421 tcggcccgcg tgatcccgtt caaaggttcg tggatcgaat ttgctacgga catcaacaac
     9481 gtgatgtacg cctatatcga ccggaagaag aaattaccgg tgaccacgct gctgcgcgct
     9541 atcggctggg aaagcgataa gcagatcctc gagctgttcg gactcgccga cgaagtgaag
     9601 gtcagcaaag ccggactcaa agccgttgtc ggaagaaagc tcgccgcacg tgtgctgaaa
     9661 acatgggtgg aggattttgt cgatgaagat accggtgaag ttgtttccat cgagcgtaac
     9721 gaagttatca tcgaccgcga aacgattctc gataacaacc acattgatct tatcgttgat
     9781 gcgaatgtca agtccatcat cctgcacaag gaagatgcgg ccaacctcga ttattccatc
     9841 atttacaata cacttcagaa ggatacgtcc aactctgaaa aagaagctgt ggagcacatt
     9901 taccgccagc ttcgcaatgc cgaaccgccg gatgaagaaa cagcccgcgg tattattgac
     9961 aagctgtttt tctccgacaa gcgttacgac ctcggtgaag tgggccgcta caggctgaac
    10021 aaaaaactca agctgaacac agcggaagac gttcgcgtcc tcacgaaaga cgatatcatc
    10081 gccattatca aatacctgat cgaactgatc aattccaagg ctgatattga cgatatcgac
    10141 catctttcca accgccgcgt ccgtacggta ggcgagcagc tctattcgca gttcggcgtc
    10201 ggtcttgcgc gtatggcgcg tacaatccgc gagcgcatga acgtgcgcga taacgaagta
    10261 ttcacaccga ccgacctgat caacgcgaag acgctttctt cggtgatcaa ttcgttcttc
    10321 ggaacgaacc agctgtcgca gttcatggac cagacgaacc cgcttgcaga gatcacgcac
    10381 aagcgtcgtc tctccgcact cggaccagga ggtctttccc gcgaacgtgc cggtttcgag
    10441 gtgcgcgacg tacactacac gcattacggc cgtctctgca cgatcgaaac gccggaaggt
    10501 ccgaacatcg gacttatttc ttccctctgc gtttatgcga aaatcaacaa gctcgggttc
    10561 attgaaacac cttaccgcac agtaacgaac ggtaaagtgg acgtgaacaa gcatgttgtt
    10621 tacctgactg cagaagaaga agatcagaag atcattgcac aggcgaatgt cgatgtcaac
    10681 gacaaaggac agttcgccag caaccgtgtg aaagcgcgct acgaatcgga ttacccggtc
    10741 gttgaaccgg agcagatctc gcttatggac gttgctccga accagatcgc ctccatcgcc
    10801 gcttcgctca tcccgttcct tgagcacgat gacgccaacc gcgcactcat gggatcgaac
    10861 atgcagcgcc aggcagttcc gctgcttcgc ccgcaggctc cgatcgtggg aaccggcctc
    10921 gaaggtctca tcgcgcgcga ctcacgcgtg ctgtggcagg cggaaggaga cggcgttgtg
    10981 gaatatgtcg attcaacgga gatcacgatc cgctataacc gctcggataa agaccgcctc
    11041 atcagctttg atgatgatgt gaagtcttac cgcctcacca aattccgcaa gacaaaccag
    11101 agcacatgca tcaacatccg cccgatggtg aagaaaggcg ataaagtgaa gcgcggacag
    11161 atcctgtgtg aaggctatgc aacgaaagat tccgagatcg cactcggacg gaacctgaaa
    11221 gtggcgttca tgccgtggaa gggttacaat ttcgaggatg ccatcgtgat cagcgagcgc
    11281 gtggtgcgtg atgatatttt cacgtccatc cacattgatg aatacgtgat ggaagtgcgc
    11341 gacaccaagc gcggactcga agagcttacg gcggatatcc cgaacgtttc tgaagaagcg
    11401 acgaaagatc tcgacgagaa cggcctgatc cgtgtcggtg cagaagtgaa ggaaggcgat
    11461 attcttatcg gcaagatcac gccgaaggga gaaaccgacc cttctccgga agaaaagctg
    11521 ctgcgcgcca tcttcggcga caaagcgggc gacgtaaaag acgcttcgct gaaagttccg
    11581 ccgtccatcc gtggcgtcgt catcgacaaa aaacttttct caagagcgat caaggataaa
    11641 aaggcgaaag cagaagaaaa aactcttctc gaaaagatcg agaaagagga agcagttgaa
    11701 tcagctgctc tccgcgatac gcttatccag aagctgtctt ctattctcaa cggaaaaact
    11761 tctgcaggtg ttttcaataa cctgaaagag gaagtcgttg aaaaaggaac gaagttcagc
    11821 gggaaagttc tcgaaaagat cgatttcaat aacgtgaacc cggcgcgctg gacgaacgat
    11881 aacgatacga acgagctggt gaagatcacg ctccacaatt tcgggatcaa attcaacgac
    11941 ctcggcggcg tttacaaacg caaaaaattc gcactgcagg tcggtgacga gcttcctgca
    12001 ggaatcgtac agcttgccaa agtttatatc gccaagaagc gcaagctcaa agtgggcgat
    12061 aaaatggcag gacgtcacgg taacaaggga attgttgccc gtatcgtgcg cgatgaagac
    12121 atgccgttcc tcgaagacgg aaccccggtt gacattgtgc tcaacccact cggcgtaccg
    12181 tcacgtatga accttggaca gatctacgaa actgttctcg gatgggccgg gcaaaagctc
    12241 ggaatgaaat tcgccacgcc gatcttcgac ggagctacca ttgatgagat caacaagtac
    12301 acggacgaag caggaattcc gcgctacggc cgtacgtacc tgtatgacgg tggaaccggc
    12361 gagcgtttcg atcagcctgc cacggtgggc attatctaca tgctcaaact gggtcacctt
    12421 gtcgatgaca agatgcacgc acgttcgatc ggaccttact cgcttatcac acagcagccg
    12481 ctcggtggta aagcgcagtt cggcggacag cgtttcgggg aaatggaagt gtgggcgctc
    12541 gaagctttcg gtgcggccaa cattctccag gagatcctca ccatcaagtc ggatgacgtt
    12601 gttggtcgtg cgaaagcata cgaagcgatc gttaagggtg aaaatatgcc gacacccggc
    12661 ataccggaat ccttcaacgt gttgctccat gagcttcgcg gactggcgct caacgtcaat
    12721 ctcgattaat tgaacggtca ctgattcaac gaacaaaaaa atcagcaata tgtctttccg
    12781 aaaagaattc aaaataaaga gcaacttctc gacgatcacg atcagcctgg cttccccgga
    12841 atccattctc gagcgttcga gcggagaagt gctcaagccg gaaacgatca actatcgtac
    12901 gtacaagccc gaacgcgacg gcctgttctg cgaaaggatc ttcggaccgg ttaaagactg
    12961 ggaatgtcat tgcggcaaat acaagcgcat ccgttacaaa gggatcgttt gtgatcgctg
    13021 cggtgtcgaa gtgacggaga aaaaagttcg ccgtgaacgc atgggccaca tcaatcttac
    13081 tgttcctgtt gcgcacatct ggtatttccg ttcgcttccg aacaagatcg gttatcttct
    13141 cggccttccg accaagaagc tcgacatgat catttactat gagcgttatg tggtgatcaa
    13201 cgccggcgtg aaagcggcgg acggaatcaa ctacatggat ttcctgacgg aagagcagta
    13261 ccttgaaatt ctcgatacgc ttccgaaaga gaaccaatac ctcgacgaca acgatcccaa
    13321 caaattcatt gcgaaaatgg gagccgaagc gttgtacgat cttctccagc gcatcaagct
    13381 ggatgagctt tcgtacgagc tccgtcacaa agcaaatacc gaaacttcgc agcagcgcaa
    13441 gaacgaagcg ctgaagcgcc tccaggtagt ggaagcgttc cgccacgcga acaagaacgt
    13501 ggagaatcac ccggaatgga tgatcatcaa ggtcgtcccg gttattccgc cggaacttcg
    13561 cccgctcgtt ccgctggatg gcggccgttt cgcaacgtcg gatctcaacg acctctaccg
    13621 ccgtgtgatc atccgcaaca accgtctcaa gcgtcttatc gagatcaaag ccccggacgt
    13681 aattctccgc aacgagaaac gtatgctcca ggaagcggtt gattctctct tcgacaactc
    13741 gcgcaaatcg aacgccgtga aaacggaatc gaaccgcgca ctgaagtctc tttccgattc
    13801 actcaaagga aagcaaggcc gtttccgcca gaacctgctc ggaaaacgcg tcgattattc
    13861 ggctcgttcc gtaattgtcg tcggacctga actgaagctg cacgagtgtg gtcttccgaa
    13921 agagatggcg gccgagctgt tcaagccgtt tatcatccgc aagctcatcg aaaggggaat
    13981 cgtgaaaacg gtcaagtcgg cgaaaaaaat tgtggaccgc aaagacgcag tcgtttggga
    14041 tattcttgaa aatgtattga aaggacatcc tgttctgctc aaccgcgctc ccacgctgca
    14101 ccgtctcggt atccaggctt tccagccgaa acttatcgaa ggaaaagcga tccagcttca
    14161 cccgctcgtg tgtacggcat tcaacgccga cttcgacggt gaccagatgg ccgtgcacgt
    14221 tccgcttgga aatgccgcta tccttgaagc acagatcctg atgcttgcat cgcacaacat
    14281 tctcaacccg gccaacggcg caccgatcgc ggttccgtcg caggacatgg ttcttggtct
    14341 gtactacatg accaagggaa tgaagaccga tgacaagatc acggtgaaag gacagggtct
    14401 cacattctac aacgcagaag aagcgatcat cgcctacaat gaaggccgtg ttcacctgaa
    14461 tgcgtttgtg aaagtgaaag tgaacaatgc gcgtgaagaa ggaaagcttg taacgaaagt
    14521 gatcgaaaca acgctcggaa gaatcctgtt caaccaggta gttcccgcgg aggttggtta
    14581 catcaacgag ctgctgacga agaagtcact ccgtgacatc atcggcaacg tgctgaaaga
    14641 aaccggcatg gcaaaaacgt cgcagttcct cgatgatatc aaggagctcg gtttccgcat
    14701 ggcgttcaaa ggcggtcttt cgtttaacct cggtgatatt atcgtgccgg aagaaaaaga
    14761 gaagatgatc gccgaagcga acgaacaggt ggaagaggtg aagtcgaact acaacatggg
    14821 cttcatcacc aacaacgaac gttacaacca gatcatcgat atctggacac ataccaacaa
    14881 ccgcatcacc aagcgtgtgc tcgatcagct ttcttccgat cgccagggat tcaaccctgt
    14941 gtacatgatg ctggattccg gtgcaagggg ttccaaagag cagatcaagc agctcggcgg
    15001 aatgagaggt cttatggcga aaccgtcgaa agcgggttct gccggcggag agatcatcga
    15061 aaacccggtc atctccaact tcaaggaagg tctgtcgatc ctcgaatact tcatctcgac
    15121 gcacggtgcg cgtaaaggtc ttgcggatac cgctcttaaa acagctgacg caggttacct
    15181 cacacgccgt ctcgttgacg ttgcgcagga cgtgatcatc acggaagaag attgcggaac
    15241 gcttcgcggt cttgttgcca ccccgctcaa aaagaacgaa gagatcgtcg agccgctgca
    15301 tgaccgcatt ctcggtcgta ccacggtgca tgacgtttac gatccgcaaa ccggcgagct
    15361 catcatcgaa agcggaaatg agatcaccga ggaatatgca tcccgtatcg accagacggc
    15421 tatcgaatcg ctggagatcc gttccgtgct gacctgcgaa tcgaagcagg gcgtatgcgc
    15481 aaaatgctac ggaagaaacc ttgccacggg ccgtatggtc cagcagggcg aatcggtcgg
    15541 tgttattgcg gcgcaatcca ttggtgaacc gggaacgcag cttacgcttc gtacgttcca
    15601 cgtcggtgga acagcttcgg gtaccgccac cgcttccaag ctggaagcga agtacgacgg
    15661 tatcatcgag atcgaagagc ttcgcacggt gaagcgcaaa accgaaaacg gaccggtcga
    15721 tgttgttatc ggtcgttctg cagaaatgcg catcatcgac acgaacacgc agatcgttct
    15781 taccacggga aatattcctt acggttccca gctgaacgtg aagaacggtg agacggtgaa
    15841 gaaaggccag actatctgcg aatgggatcc gtataacgcg gttatcgttt ccgagttcgg
    15901 cggcaagctg gaattcgagc acatcgagga aggcattacg ttccgtgaag aagccgacga
    15961 acagaccggt ttccgcgaga aggtgatcat cgaatcgaaa gacaaaacga agaacccgtc
    16021 catccgcatt gtcagcggca gcgatatcct gaaaacatac aacatcccgg tttcggcgca
    16081 catcgttgtt gacgacggag cgaagatcga ggcgggccag atcctcgtca agatcccgag
    16141 agcgctcgga aaatcaggcg atatcaccgg cggtctgccg cgtgttacgg aacttttcga
    16201 agcacgtaac ccgagcaacc cggctgttgt ttccgagatc gacggcgtgg tcaccttcgg
    16261 caagatcaaa cgtggtaacc gcgaggtgca cgtcgagtcg cgcacgggtg aacgcaaaac
    16321 ctatctcgtt gccctttcca agcacatcct tgtgcaggaa aacgatttca tccgtgcagg
    16381 tgaagcgctt tccgacggat cgatcagccc gggagatatt ctcgcgatca aaggtcccac
    16441 agctgtgcag gaatatcttg tgaatgaagt ccaggaagtt taccgcctgc agggtgtaaa
    16501 gatcaacgac aagcatttcg aggtgatcgt tcgccagatg atgcgcaaag tcgagatcgt
    16561 cgatccgggc gataccatgt tcctcgagca gcagattgcg aacaagaccg acttcatgga
    16621 agagaacgac cgtctcttcg gcaagaaggt tgttgttgat tccggcgatt cttcatcgat
    16681 gaaatcagga cagatcattt ctgcgcgcaa gcttcgtgat gagaattcca tgctcaagcg
    16741 caaggacatg aagcaggtgg aagcgcgtga tgcggttccc gcaacgtcct cgcagatcct
    16801 gcagggtatt acgcgtgcgt ctctccagac gaacagctgg gtttctgcgg catctttcca
    16861 ggaaacgacg aaagtgctca acgaagcggc tgtcaacggg aagcaggacc atctccttgg
    16921 cctcaaggaa aacgttatcg tcgggcacct tatcccggcg ggtaccggaa accgcgctta
    16981 cgagaagctc gttgtcggat caaaagacga atacgagaag ctcatggccg cgaagaaaga
    17041 ggcgatggaa gaagaagtgt aacaccagtt tatgtaatca caagacaagg ttggctgaaa
    17101 agtcaacctt gtttttttat attgccttcg aaatggaaac ggtaaaaaca gccggcaggg
    17161 aagacctcgg tttcgctctg tacgaacgcc gggaggatgg cgtgatcgtg ctgcacctgg
    17221 aaaatgatct tatgatcgac ctggaaaaag cggagcggat gaatgaggcc ctgggaagga
    17281 tcacggcagg aataccgtcg aagatactcg tgctcaccgg gaaatacagc tcggcagaca
    17341 aagaggcgag gcagctgctg tcatcgggtc agaaacgttt gcagatcgcg aaggcgggag
    17401 ttgttattca ttcgctttcg cagaagctgc tggccaattt tttcagcaat gtgcacaagc
    17461 ctcctttccc ggtccggttt ttcaattctg cggaagatgc ggagaaatgg cttcgcgact
    17521 gatgcgcctt tcgcatgaag cacacgccta tttttccgtt caaaacgccc gcgaaaatta
    17581 tcgcg
//
LOCUS       DASTSF010000045         5930 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_857656,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000045 DASTSF010000000
VERSION     DASTSF010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5930
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_857656"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..373
                     /locus_tag="VFU15_01405"
     CDS             <1..373
                     /locus_tag="VFU15_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HEU4716451.1"
                     /translation="ENVKRNNTEAIEATHRLKEQARLMKECLLRGETDGIGEILNLGW
                     LNKKKMAASISNSTLDDIYEAALAAGATGGKISGAGGGGFMFFYCPGTSRLNVALALA
                     QFGGRTKSFTFTQQGLTTWTN"
     gene            361..948
                     /locus_tag="VFU15_01410"
     CDS             361..948
                     /locus_tag="VFU15_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020571672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HEU4716452.1"
                     /translation="MDQLNFVRSKIEASIRLKTALLSDEPLLKTITAITNSVISCYKA
                     GGKVLWCGNGGSAADAQHLAAELSGRFYYDRPPLFSEALHVNTSYLTAVANDYSYGQV
                     YGRLVQAMGKKGDILIGLSTSGNSENVVTALIKAKELGMITAGFTGETGGKMKPHCDF
                     LVNIPSTDTPRIQECHMILGHTLCEMVEMELFPKK"
     gene            954..1373
                     /locus_tag="VFU15_01415"
     CDS             954..1373
                     /locus_tag="VFU15_01415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716453.1"
                     /translation="MKHLLLLIPGFFLLCSCNGSYTRTNMPGDISDGKALAEKFYTFL
                     AKHSVPGINTISSGIKPDEMNHLMNFADSSWGAMKSYEFTSGKSQIQKNNGVRSGEID
                     LYYKANYERSVNKETIIIEVVNDSLKVTGYDTHPDKQ"
     gene            1413..2126
                     /locus_tag="VFU15_01420"
     CDS             1413..2126
                     /locus_tag="VFU15_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017733576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEU4716454.1"
                     /translation="MLKEVIILAGGFGTRLRSVIAGIPKPMADISGKPFLHYLLSHVQ
                     KAGAEKVILSVGYKAEIIEAHFGRNYLGMEIIYAFENEPLGTGGGIRLAMEKCEGENV
                     LTMNGDSFFGISLNALSEFHAQHNADATLALRKVSDASRYGTIETGTGSRILSFREKN
                     PAVTGEALINAGVYLLRRELFLGKTRGGKAFSIENDFFAPNAATLRFYGMKSDDYFID
                     IGIPEDYERAKKEFVQLYS"
     gene            complement(2210..3811)
                     /locus_tag="VFU15_01425"
     CDS             complement(2210..3811)
                     /locus_tag="VFU15_01425"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014681619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HEU4716455.1"
                     /translation="MKKVLTLFAVALSVALSAQMNGVNQKAPENWFNLSYSTDHVHGI
                     GTDRTYADLIQGKKPDTVIVAVIDGGVDYTHEDLKDVMWHNPGEIPDNGIDDDHNGYV
                     DDVYGWNFIGGKDGKDVQYDNLELTRLLRPLDKKFNGRDAASIQPAEKDEYNRYLKLK
                     ADFDKRRADSQKTFDQISNFKRKMDGMKHEIKDQRKVDSVTYADYKAYTPSDQYKRLA
                     MVLNLVCKNEDDWNNFNTEINEGYDELDAMLKYNLNLDYDPRSIVGDNYDDQTEKYYG
                     NNDVKGPDALHGTHVAGIIAANRGNNLGIKGVNSAVKIMAVRVVPNGDERDKDVANGI
                     RYAVDNGAKIINMSFGKGYSFNKKVVDDAVKYAESKGVLLVHAAGNDSKNNDNTDNFP
                     NDHYLDGGTASNWIEVGALSWKDGKEAVAPFSNFGAAQVNVFAPGVDIKSCKSNGGYI
                     DESGTSMASPVTAGACALIKCYFPSLTPQQIKTIIEQSSDKSLAKKRVIKPGYKKKKV
                     KFKKLSTSGGVVDVYAAFRMAETMK"
     gene            complement(3918..5057)
                     /locus_tag="VFU15_01430"
     CDS             complement(3918..5057)
                     /locus_tag="VFU15_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018476786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEU4716456.1"
                     /translation="MQFSLTENQHMIAEMVRKFGEENIRPKMMEWDESQEFPVDVMKK
                     LGDLGLLGVLVPQEYGGSGFGYLEYVTAISEIGKICGSIGLSTAAHNSLCTGHILQFG
                     NEEQKKKYLPKLATGEWIGAWGLTEQNTGSDAGGMLTTAVADGDHFVINGSKNFITHA
                     ISGDVAVVIVRTGEKGDSHGMTAFIIEKGTPGFRPGKKENKLGMRASETAELVFDNCR
                     VHKSQMLGKTGEGFIQAMKVLDGGRISIAALSIGIAKGAYEAALKYSKERQQFGKPIA
                     AFQAIAFKIADMATEIDAAELLTFQAADIKNRGGKVTRESAFAKYYASEVAVRVSTEA
                     VQIFGGYGYTKDFPAEKYYRDSKLCTIGEGTSEIQKLVIARELTR"
     gene            complement(5194..5715)
                     /locus_tag="VFU15_01435"
     CDS             complement(5194..5715)
                     /locus_tag="VFU15_01435"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013454927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEU4716457.1"
                     /translation="MESIRNIIRNVPDFPKPGILFKDITPLLQDPKTGAAIISEFRKR
                     LEGVRVDAILGVESRGFLFGYALALQMGVPFVPVRKSGKLPWKTIGTEYELEYGKAKI
                     EMHEDALKPGSKVIIHDDLLATGGTAAAAARLVQMAECEVSAFAFVVELGFLEGRKNL
                     ADHSGTVISLVEY"
     gene            complement(5716..>5930)
                     /locus_tag="VFU15_01440"
     CDS             complement(5716..>5930)
                     /locus_tag="VFU15_01440"
                     /inference="COORDINATES: protein motif:HMM:NF024245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEU4716458.1"
                     /translation="TADSSYIQKININSATVAELKKHPYFTYNVAQALVNYRKVHGPF
                     HSVADVTGCVLVNADLYRKIAPYLSI"
BASE COUNT         1380 a         1570 c         1529 g         1451 t
ORIGIN      
        1 ggaaaatgta aaacgcaaca atacggaagc gatcgaagcc acacaccggc tgaaggagca
       61 ggcaaggctg atgaaggaat gcctgctgcg cggagaaacg gacggcatcg gcgaaatcct
      121 gaacctcgga tggctgaaca agaaaaaaat ggcggcctcg atctcgaaca gcacgcttga
      181 cgacatctac gaggcggcgc ttgccgcagg tgcaacaggc ggaaagatat ccggggcggg
      241 cggcggcgga ttcatgtttt tctactgccc gggaacaagc cgtctcaatg tcgcgctcgc
      301 gcttgcgcaa ttcgggggcc ggaccaaatc atttacattt actcaacagg gattaaccac
      361 atggaccaac tgaactttgt ccgctcgaaa atagaagctt caatcaggtt gaaaacagcg
      421 ttgctttcag acgaaccgct gctgaaaacg ataaccgcga ttactaattc cgtgatctcc
      481 tgctacaaag ccggcggaaa agtgctctgg tgcggcaatg gcggcagtgc ggcggacgcg
      541 cagcatcttg ccgccgagct ttcgggacgt ttttactatg accgtccgcc tcttttttcg
      601 gaagcgctgc acgtcaacac ttcctacctg acggccgttg caaacgatta cagctacggg
      661 caggtgtacg gccgtctcgt ccaggccatg ggaaagaagg gcgacattct catcgggctt
      721 tccaccagcg ggaattccga aaatgtagta accgcgctta ttaaagcaaa agaactggga
      781 atgatcacgg ccgggttcac cggtgagacc gggggaaaaa tgaaaccaca ttgcgatttc
      841 ctggtcaata ttccctcgac cgatacgcca cgcatacagg aatgccacat gatcctcggg
      901 catacgctgt gcgagatggt ggaaatggaa ttattcccta aaaaataaac aacatgaaac
      961 atctgctcct gcttattccc ggatttttcc tgctttgctc ctgcaatggt tcctatacac
     1021 ggacgaatat gccgggtgat atcagcgacg gaaaagcgct tgcggaaaaa ttctacactt
     1081 ttctcgcgaa acattccgtg cctggcataa acacgatcag ttcgggcatc aaacccgacg
     1141 aaatgaacca cctgatgaat tttgcagaca gcagctgggg cgccatgaaa agctacgagt
     1201 tcacctcggg gaaaagccag atccagaaaa acaacggtgt gcgttccggg gagatcgacc
     1261 tgtattacaa agcgaattac gaacgctccg tcaataaaga aacgatcatc attgaagtcg
     1321 taaacgattc gctgaaagta accgggtatg acacgcatcc cgacaaacag taaaccccgt
     1381 cccaagaagg aacgcaatca cgatctttcg gcgtgctaaa agaagtcatc atactggccg
     1441 gcggattcgg cacgcgactg cggtctgtga tcgccggtat tcccaaaccg atggctgaca
     1501 tttccgggaa gccgttcctg cattacctgc tatcacatgt gcagaaagcc ggcgccgaaa
     1561 aagtgatcct gtcggtcggc tacaaagcag aaatcatcga agcgcatttc ggcaggaatt
     1621 acctcgggat ggagatcatt tacgctttcg agaacgaacc gctcggcaca ggcggaggaa
     1681 tccgcctcgc gatggaaaaa tgcgaggggg aaaatgtatt gacaatgaac ggggattcct
     1741 ttttcggcat ttcactgaac gcactttcgg aatttcatgc gcaacataac gcagacgcaa
     1801 cgctcgcgct gcgaaaagtg agtgatgctt cacgttacgg gaccatcgaa accggcaccg
     1861 gctcacggat actttcattc cgtgaaaaaa atccggccgt aaccggcgaa gcgctgatca
     1921 atgccggcgt ttacctgctc cgccgggaac tgtttctcgg gaagacgcgc ggtggaaaag
     1981 cattctccat cgagaacgat tttttcgcac cgaatgcggc gacgcttcgt ttttacggaa
     2041 tgaaatcgga tgattatttt atcgacatcg ggataccgga agattatgag cgggcgaaaa
     2101 aggaattcgt gcagctgtat tcctgaaaaa tgcttttcat tttcagcaga aagaaaaaag
     2161 cgcagaagag ctgaactccc ctgcgctttc cgtttctttc caacgcgtgt tacttcattg
     2221 tctccgccat cctgaacgcg gcgtacacat ccaccacgcc accgctcgtg ctcagctttt
     2281 tgaacttcac tttcttcttt ttataaccgg gcttaataac ccgtttcttc gccagcgatt
     2341 tatcggacga ttgctcgatg atcgttttga tctgctgcgg cgtcaatgaa gggaaatagc
     2401 atttgatcag cgcgcaggcg ccggcagtaa ccggggaagc catgctcgtt ccactctcgt
     2461 cgatgtaacc gccgttgctt ttgcaggatt tgatatcgac acccggagcg aatacgttca
     2521 cctgtgcggc gccgaaattg ctgaaaggcg cgaccgcctc tttcccgtct ttccaggaaa
     2581 gcgcaccgac ctcgatccag ttggaagccg ttccgccgtc gaggtaatga tcgttcggga
     2641 aattgtcggt gttgtcgtta ttcttgctgt cgttgcctgc tgcgtgaacg agaagaacgc
     2701 ctttcgattc cgcgtatttc accgcatcat ccacaacttt cttgttgaac gaatagcctt
     2761 tcccgaagct catgttgatg atcttcgcgc cgttgtcgac cgcgtaacgg atcccgttcg
     2821 ccacgtcctt gtcgcgttcg tcgccattgg gaaccacacg aacggccatg atcttcacgg
     2881 ctgaattcac gcctttgatg ccaaggttat tcccgcggtt cgcagcgata attccagcca
     2941 cgtgcgtgcc gtgcagcgca tccgggcctt tcacatcatt gttgccataa tatttttccg
     3001 tctggtcgtc gtaattatcg ccgacgatgg aacgcggatc gtaatcgagg ttaagattat
     3061 acttgagcat cgcgtcgagc tcgtcataac cttcgttgat ctcggtattg aaattattcc
     3121 agtcgtcctc gttcttgcag acgaggttca gcaccatcgc aagacgtttg tactggtcgg
     3181 aaggcgtgta cgctttgtaa tccgcatagg tcacggaatc caccttgcgc tggtctttga
     3241 tctcgtgctt catgccatcc atcttgcgct tgaagttgga tatctggtcg aacgtttttt
     3301 gtgaatcggc gcggcgcttg tcgaaatccg ctttcagctt gaggtaacgg ttgtattcgt
     3361 ccttttcagc gggctgaatg gaggcggcat cgcggccgtt gaatttttta tccagcgggc
     3421 gaaggaggcg ggtaagctcg aggttatcgt actgcacatc tttcccgtct tttccgccga
     3481 tgaaattcca tccgtagaca tcatcgacat acccattgtg atcgtcatcg atgccgttat
     3541 ccgggatctc gccgggattg tgccacatta cgtctttcag atcttcgtgc gtgtaatcca
     3601 ctcctccatc gatcacggcg acgatcaccg tatccggttt ttttccctgg ataagatctg
     3661 cgtatgtgcg gtcagttccg atgccgtgaa catggtcggt ggaataagag aggttgaacc
     3721 agttttccgg cgctttctgg ttcactccgt tcatctgcgc cgacagcgcc acactcagcg
     3781 caacggcaaa cagggtcaat acttttttca tttgtggtct gttgattttg gattaccaaa
     3841 tttagaataa aaaaacaccg tctttcgccg catgctgatg agcgggcaaa gggctttttt
     3901 aatggaaaaa aggatcatta tctggtcagc tcgcgggcaa tgaccagctt ctgaatctcg
     3961 gacgttcctt cgccgatggt gcagagcttc gaatcgcggt aatatttttc cgcaggaaaa
     4021 tccttcgtgt agccgtagcc tccgaagatt tgcacagctt cggtggaaac gcgcacggct
     4081 acttcagacg cataatattt cgcgaatgcg gactcgcgcg tcacttttcc gccacggttc
     4141 ttgatgtcgg cggcctggaa cgtaagcagc tccgctgcat cgatctccgt ggccatgtcg
     4201 gcgatcttga acgcgatcgc ctggaaagcg gcaatgggct tgccgaactg ctggcgttct
     4261 ttcgaatatt tcagcgcggc ttcgtacgct cctttcgcga tgccgatgga taatgcagca
     4321 atggaaatcc gtccgccgtc gagcactttc atcgcctgga taaaaccttc cccggttttc
     4381 ccgagcatct gcgatttatg cacgcggcaa ttatcgaata cgagctccgc ggtttcggag
     4441 gcgcgcatgc cgagcttgtt ctcttttttt cccggtctga acccgggtgt tcctttttcg
     4501 atgatgaagg cggtcatgcc gtgcgaatcg cctttttcac ccgtgcggac gatgacgacc
     4561 gccacgtcgc cggaaatggc gtgggtgatg aaattcttcg aaccgttgat cacgaagtga
     4621 tcgccatctg caactgccgt ggtaagcatt cctcccgcat cagaaccggt gttctgctcg
     4681 gtgagtcccc atgcgccgat ccattcaccg gtggcgagct tcggtaaata ttttttcttc
     4741 tgctcttcgt tcccgaactg gagaatatgt cccgtgcaga gcgaattatg ggcagccgtg
     4801 gacaggccga tcgaaccgca gatcttcccg atctcggaaa tagcagtgac atattcgagg
     4861 tagccgaaac ccgatccgcc gtattcctgt ggaacgagca cgccgagcag accgagatcg
     4921 ccgagctttt tcatgacgtc cacggggaat tcctgcgact catcccattc catcattttc
     4981 ggccggatat tttcttcacc gaacttccgc accatttcgg cgatcatgtg ctggttctct
     5041 gtcagggaaa attgcatggg aaaattagtt tttgaacagg gacaaaacta aggtttttac
     5101 ctcacggaaa acgttaaaca aatcttcatt tttcgattac cgcgggatgc agccgcatga
     5161 tccctgccgt tcccgcagga aggaaaagac ggatcagtat tcaacgagac tgatgaccgt
     5221 tccggaatgg tccgcgaggt ttttgcggcc ttcgaggaag ccgagctcga caacgaatgc
     5281 aaatgcagaa acttcgcatt ccgccatttg cacgagtcgc gccgcagcag ccgctgttcc
     5341 gcccgttgcg agaagatcgt catgaatgat gactttcgag ccgggcttca gcgcatcttc
     5401 atgcatttca attttcgctt tgccgtattc gagttcgtat tctgttccga tggttttcca
     5461 gggcagcttt cccgatttgc gcacagggac aaaaggcacg cccatctgca gcgcgagcgc
     5521 atagccgaac aggaatcccc ggctttccac gccgagtatc gcatcgacgc gaacaccttc
     5581 caggcgtttc ctgaactcgg aaatgatcgc tgcgccggtt ttggggtcct gcagcaaagg
     5641 tgtgatgtcc ttgaaaagaa ttcccggctt cggaaaatcg ggaacgttgc ggatgatgtt
     5701 gcgaattgat tccactcaga tcgaaaggta gggtgcaatt ttacggtata aatccgcatt
     5761 gaccaaaaca catcctgtta catcagcgac cgaatgaaag gggccgtgga ccttccggta
     5821 attcacgagc gcctgcgcga cattgtacgt gaaatacgga tgctttttca gctccgccac
     5881 ggtcgccgag ttgatgttga ttttctggat gtaggaagaa tccgccgtga
//
LOCUS       DASTSF010000046         7715 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1751666,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000046 DASTSF010000000
VERSION     DASTSF010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 7715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7715
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1751666"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(110..736)
                     /locus_tag="VFU15_01445"
     CDS             complement(110..736)
                     /locus_tag="VFU15_01445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716459.1"
                     /translation="MKPPAASLIIAICLCFCSCGLIHSVEFEKRIYRKGFYFHRDRTL
                     PSRRNTVRYNPDKQITIDGFALPGPANEKCAITIPAKTPADILIKKPSGSLNGKTTKQ
                     LPIPLAHPGHNFHLHAVRGDKAAVEDWILSIICCFLLPFFSILVYAFAYNNDSKYTKV
                     LQGLSFLWFFAWITAIILFFFILPLAVILYSCLVAVSFVFMFLNKSYD"
     gene            complement(761..2071)
                     /gene="murF"
                     /locus_tag="VFU15_01450"
     CDS             complement(761..2071)
                     /gene="murF"
                     /locus_tag="VFU15_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012780257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEU4716460.1"
                     /translation="MTFTDIPTLYGHYLRHPLVSTDTRTIPQGSLFFALKGGNFNGNK
                     FAAQALEKGAAFAIVDEAECVSGDRFLLVPDVLQALQELARFHRDSLGKKGLNVFALT
                     GSNGKTTTKELLARVLSVKYRTLFTQGNLNNHIGVPLTLLQLNETHEMAVVEMGANHQ
                     GEIRRLCGIADPDFGLITNIGLAHLEGFGGPEGVLKGKTELFEHLRKKNGTAFVLADD
                     GRVMKIAEGMKTITYGTNQHAGVRGNFISADPFVRFSWETDSVTAHEVESRMVGAYNL
                     TNLLAACAAGFYFGVPATEIDEAIASYIPSNARSQMEKRGSNTLILDCYNANPSSMEA
                     AIENISKMPAERKILVLGDMFELGEESAKEHQRIVNFIGEKTKSATVLLIGKLFGATK
                     DDFGGIRLADTAEAAQWMKKNQPENSLILIKGSRGMKMENVLAD"
     gene            complement(2135..3688)
                     /gene="gldJ"
                     /locus_tag="VFU15_01455"
     CDS             complement(2135..3688)
                     /gene="gldJ"
                     /locus_tag="VFU15_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010527013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility lipoprotein GldJ"
                     /protein_id="HEU4716461.1"
                     /translation="MKKAAMPLMLIAGLSFLASCQKERSNVTGWNYNDPNNGGFEVVP
                     YEEQETGPGLVFITGGTFTMGRVEQDVNYDWNNVPRRVTVSSFYMDETEVRNLDYLEY
                     LYWTDRVFGADYPEIYVQALPDTLCWREKLAYNEPYVLYYLRHPAYRDYPVVGVNWLQ
                     ASNFCSWRSDRVNEFILIREGVLDWAPDQVNEAAFNTDHYLAGTKANDPYVGNVVQNI
                     PSLNPTGPEERMVRMEDGILLPKYRLPTEAEWEYAAYGLIGNSIDERVTDRRLYPWNG
                     HAVRNPDERYIGQMLANYQRSNGDMMGTAGLLNDAADVTAPVYSYWPNDYGLYNMAGN
                     VSEWVMDVYRPQSLTDDNDFRAFRGNVFQTQMKDPTDPNFVMVEDSVRYDADSNIISI
                     PGAIRYRNVDISYKEDKLDTRRNYKYADNINYKDGDLVSSLYYSDPSTNPDKMMYEFP
                     DAKNQSSTAGSMINNKAHVYKGGSWHDRAYWMVPGTRRFLDQQQDLSWLGFRCAMDRV
                     GSPQGLGGK"
     gene            complement(3777..4730)
                     /locus_tag="VFU15_01460"
     CDS             complement(3777..4730)
                     /locus_tag="VFU15_01460"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HEU4716462.1"
                     /translation="MLKRLVIALALVATAGLGDLFAQDPEFTQFYANPLYLNPAFAGT
                     ARCPRVCLNYRNQWPGITGTFVTYSASYDQHIDGISGGLGLLVTNDRAGQATINTTNI
                     SGIYSYQLNITREFSMKFGVQATYAQKSVDWSKLNFGDMIDPRRGFIYNTNEVPNLTS
                     KTNVDFSAGILGYSRHYFFGFAVHHITEPDEGFLGVSKLPMKYTGHAGAVIPLGGRYG
                     DSNISPNILFQKQQDFQQLNLGVYFTKGPIVGGLWYRNQDSFILLIGFQQDLFKFGYS
                     YDVTVSKLTNATAGSHELSFQMQFQCKPKKKKFRTVSCPSF"
     gene            5083..>7715
                     /gene="porU"
                     /locus_tag="VFU15_01465"
     CDS             5083..>7715
                     /gene="porU"
                     /locus_tag="VFU15_01465"
                     /inference="COORDINATES: protein motif:HMM:NF033707.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system sortase PorU"
                     /protein_id="HEU4716463.1"
                     /translation="MYNPDQHYLRWTAPITTQFTDQESRTFLWFDGASYDAERNFLPY
                     FFQKQKISDGTSSATATLVDPVYEALDQASLSVMGFGAGYIGSQPEVKTTVSISRKET
                     FANVTVLPFRRNPVTGAMERLVSFQLRISENGRRDARFGVTPQFANNSVLATGTWYKI
                     GVVNTGVQKIDYNFLSNMGIDMSALDPRNIRIYGNGKGQLSFKNSVAHEDDLQEYSIV
                     VQGEADGVFDTQDYILFYGISPNSWTFDSTDMHFHHHVHDYSDTTYYFLNTDLGPGKR
                     VTQRSSSSLTPTDVVTSFDDYKFFESNNTNLIKSGREWYGEEFDIINQYSYQFNFPNI
                     DPSSPACAEVDLVSRCDAQNYYTVTAQSTSATITISNTSSSCYYCPFANPGNAKLCFN
                     PGGPSILVTVTRQANQYAVTGWMNWVRVNARRFLTMTNGQMFFRDSRSAGPGKVSQFV
                     MGNYNASQTIWDVTDFANIYQQNGTVGGGNYTWVVPTDSLHEFVCFDGTQYETPVYHG
                     PVANQNLHALQPADLIIISYPDFLPQALELAQFHATMQNFSYVIVTPQQIYNEFSSGE
                     QDATALRDFVRMLYWRAGSPSQTPRFLLLFGDGSYDNKHRVAANSNFIPTFQNLNSVG
                     LTESYTSDQFYTLMDSNEGEWDDSSDNGDCDIGVGRLPVRNTTEAEDMVNKVKHYMTM
                     PQPVYNLSACSAAGQCSKGGEWRNCVTFIGDDEDNDIHMSQADQLATYVSTNYPTYNI
                     DKIYLDAYQQEQTPGGERYPAVNAAFDQRVDKGCLILNYTGHGGEVGLAHERILEVSQ
                     INAWTNLCNMPLFVTATCEFSRFDDPARTSAGEYVLLNSNGGGIGLLTTVRLVYSTPN
                     FNLNQKFYECAFNPVNGEMPC"
BASE COUNT         1835 a         1971 c         2013 g         1896 t
ORIGIN      
        1 tcccgtgctc gcttcgtctt tccctgctgt ggaaggatat gctctgtaag ttctgttgtt
       61 taacgctggc aggaaaacat ttcacgcatt ttcggattgt cctgcaacat cagtcgtagc
      121 tcttatttaa aaacataaag acaaaactca cagcaaccag gcaggaatac aggatcacgg
      181 ccagcggtag gatgaagaaa aaaagaatga tcgcagtgat ccatgcaaaa aaccagagga
      241 aagaaagccc ctgcaggact ttggtgtatt tagagtcatt gttatacgca aaagcatata
      301 ctaagatgga aaagaaaggg aggagaaagc agcagatgat ggataggatc caatcttcaa
      361 cggcagcctt gtctcctctg acagcgtgta aatggaagtt gtgaccggga tgggcaagcg
      421 ggatgggtaa ctgtttagtc gtttttccgt tcaaagagcc ggaaggtttt ttgatcaaaa
      481 tgtcagccgg tgtttttgcc gggattgtta tggcgcattt ttcatttgca ggaccgggaa
      541 gggcgaaacc atcgatggtt atttgtttat ccgggttata gcgaacggta ttccgtcggg
      601 aaggaagggt tctgtcgcgg tgaaaataaa atccttttct gtaaatgcgt ttttcgaatt
      661 caacggaatg aataagtccg catgaacaaa aacaaaggca aatcgcgata atcagggaag
      721 cagcgggcgg tttcattatc cgggttttca gattttcgca tcagtcggcc agcacattct
      781 ccattttcat tccgcggctt cctttaataa gaataagcga gttttccggc tgattttttt
      841 tcatccactg cgcagcttcg gccgtatctg ccagacgtat tcccccgaaa tcgtctttcg
      901 ttgcgccgaa cagcttaccg atgagcagaa cagttgcact ttttgttttt tctccgatga
      961 aattcacgat acgctggtgc tcttttgcac tctcttcgcc cagctcgaac atgtccccca
     1021 gcacgagtat ttttctctcc gccggcatct tcgaaatatt ttcgatcgcc gcttccatgc
     1081 tggaagggtt cgcgttgtag caatcgagga tgagcgtatt gcttccgcgt ttttccatct
     1141 gcgaacgcgc gttggacgga atgtacgaag cgatcgcctc gtcgatctcc gttgccggaa
     1201 cgccgaaata aaatccggcg gcacacgcgg cgaggagatt ggtgagattg tatgcgccca
     1261 ccatccgcga ttccacttca tgcgccgtaa ccgaatctgt ttcccagctg aaacgcacga
     1321 acggatcggc ggaaatgaaa ttcccccgca cgccggcatg ctggtttgtg ccgtacgtga
     1381 tcgtcttcat tccttccgcg atcttcatca cgcgcccgtc gtccgccagc acaaaagcgg
     1441 ttccgttttt ctttcgcagg tgttcgaaca gctcggtctt tcctttcagc acaccttcgg
     1501 gtccgccgaa accttcgagg tgcgccagcc cgatgttcgt gatcagcccg aaatccgggt
     1561 ctgcgatccc gcacaaccgg cgtatctctc cctggtgatt ggcgcccatt tccacgaccg
     1621 ccatttcatg tgtttcgttc agctgcagca gcgtcagcgg cacaccgatg tggttgttca
     1681 ggtttccctg cgtgaaaagc gtcctgtatt tcacggagag cacccgcgcc agcaattctt
     1741 tcgtcgttgt tttgccgttg ctgccggtga gcgcgaatac gttcagtcct tttttcccga
     1801 gcgaatcgcg gtgaaagcgg gccagctcct gcagcgcctg caacacgtcc ggtacgagca
     1861 ggaaacggtc gccggaaacg cactccgctt catccacgat cgcaaacgcc gcaccttttt
     1921 ccagcgcctg cgctgcgaat ttgttgccgt tgaaatttcc acccttcagc gcaaaaaaca
     1981 ggcttccctg cggaatggtg cgggtatcgg tgctgaccag cggatgacgg agataatgtc
     2041 cgtagagcgt gggtatgtct gtaaaagtca taaaagaaga acgctccaag ttacaaagaa
     2101 cccggagcgt ccgttttgat ttttcagtgg gaaattattt tcctcccagt ccctgcgggc
     2161 ttcccacgcg atccatcgcg caacggaaac cgagccagga aagatcctgc tgctggtcga
     2221 ggaaacggcg tgtaccggga accatccagt aagcacgatc gtgccaggaa ccgcctttgt
     2281 atacgtgagc cttgttgttg atcatggaac ccgcggtgct cgactggttc ttcgcatccg
     2341 ggaattcgta catcatctta tccgggttcg tggaaggatc cgaataataa agtgatgaaa
     2401 caagatcccc gtctttgtag ttgatgttgt cagcgtactt gtagttacgg cgggtatcca
     2461 gtttgtcttc tttgtaggaa atatccacgt tcctgtaacg gatggcgccg ggaatgctga
     2521 tgatgttgga atcggcatca taacgtacgg aatcttcaac cattacgaag ttcggatcgg
     2581 taggatcttt catctgggtc tggaaaacgt ttccgcggaa agcacggaaa tcattgtcat
     2641 ccgtaagcga ctgcggacga taaacgtcca ttacccattc cgaaacgttg cccgccatgt
     2701 tgtacaggcc ataatcgttc ggccagtagg aatataccgg ggctgttaca tcagcggcgt
     2761 cattcagcaa acctgcagta cccatcatgt caccattcga acgctgatag ttggcaagca
     2821 tttgcccgat gtaacgctca tccgggttac gcacggcgtg gccgttccag ggataaagcc
     2881 tgcggtccgt tacacgttcg tcgatggaat ttccgataag accgtaagct gcgtattccc
     2941 attcagcttc cgttgggagg cggtacttcg gaagaaggat cccatcttcc atgcgcacca
     3001 tgcgctcttc aggacctgtc gggttgagcg aaggaatgtt ctgcaccacg tttccgacat
     3061 aaggatcgtt tgctttcgtt ccggccaggt agtggtcggt attgaaagct gcttcattca
     3121 cctggtcagg agcccagtcg aggacgcctt cgcggatgag gatgaattcg ttcacacggt
     3181 cggaacgcca gctgcagaag ttggatgcct gcaaccagtt aacacctacg accgggtaat
     3241 cgcggtacgc agggtggcgg aggtaataca gaacgtaagg ctcgttataa gcaagctttt
     3301 cgcgccagca gagcgtatcg ggaagcgcct gaacatagat ctccggatag tcggcaccga
     3361 aaacacggtc cgtccaatag agatattcga ggtaatccag gtttctcacc tccgtttcgt
     3421 ccatatagaa cgaagaaacc gttaccctgc ggggaacgtt gttccagtcg tagttcacat
     3481 cctgttcaac gcggcccatt gtgaaagttc cgcctgtgat gaacacgaga ccgggaccgg
     3541 tttcctgctc ttcgtaaggc acaacttcaa atccgccatt gttaggatcg ttgtagttcc
     3601 agccggtaac attggaacgc tctttctggc aggaagccag gaaagagagt cctgctatca
     3661 gcatcaacgg catcgctgct ttcttcatgg ttcggtgttt tacgtcgtag tttccctttg
     3721 gtgtttaata tataacgttg attttccctt ttgttacatg tggtcctgaa aaaaaatcag
     3781 aacgaaggac agcttacggt acggaatttc ttctttttcg gcttgcactg gaactgcatc
     3841 tggaaagaga gctcgtgcga accggccgta gcgttggtca gtttggatac cgttacgtcg
     3901 taactgtatc cgaacttgaa gaggtcctgc tggaagccga tcaggaggat gaaagaatcc
     3961 tggttgcggt accagagacc accgacgatc gggcccttgg tgaaataaac accgaggttc
     4021 agctgctgga aatcctgctg cttctggaaa aggatgttcg gtgaaatatt cgaatcaccg
     4081 taacgcccgc cgagcgggat aacagcgcct gcgtgccctg tatatttcat cgggagtttg
     4141 gatacgccga ggaatccttc atcaggttcc gtaatgtggt gcacagcgaa gccgaagaaa
     4201 tagtgacggc tgtaaccgag gatgccggct gaaaagtcga cattggtttt gctggtgagg
     4261 ttgggaactt cgttcgtgtt gtaaatgaat ccgcggcgcg gatcgatcat gtcgccgaaa
     4321 ttgagcttgc tccagtcgac cgatttttgc gcataggtag cctggacgcc gaatttcatc
     4381 gagaattcac gggtgatatt cagctggtaa gaatagatcc cgctgatgtt cgtggtgttg
     4441 atcgttgcct ggcccgcacg gtcatttgtc accaggaggc cgaggccgcc tgaaatacca
     4501 tcaatgtgct gatcatagga agcgctgtag gtaacgaacg ttcctgtgat gcccggccat
     4561 tggttacggt aattgaggca tacacgcgga cagcgtgccg ttccggcgaa ggccgggttg
     4621 agatacaacg ggttagcgta gaactgagta aactcaggat cctgcgcaaa aagatcgccc
     4681 aaaccggcgg ttgccaccaa ggcaagggca ataaccaaac gcttcagcat atcggtgctg
     4741 ttaggttaag aaacaatgat acggaaaaaa gaacgcaaca gtttcaaaag ggtttatttt
     4801 ttgaagctgc ttcgcaatat ttcctggttt gcccccgttt gtactccggt ttttaaaggt
     4861 gcggaattaa cgggtaaaaa caaaagtagc gtaatttccc ggaattatca aaatcccgcc
     4921 ctctgaaaca ccatgcggtc gtctatattt acacaataaa aaaagctcag catgattcca
     4981 gtgcgtgtac gaatcgcctt ttttcgtaat ttattgataa taagtgggtt gtttgttttt
     5041 tcatgcctgg aagcgcaacg gacgggccag gtaaatggca atttgtacaa tcctgaccag
     5101 cattacctgc gatggaccgc acctatcacc acacaattca ccgaccagga atccaggaca
     5161 tttctctggt ttgacggcgc ttcctatgat gcggagcgga actttctgcc gtatttcttc
     5221 cagaagcaaa aaataagcga cggtacttct tccgcaaccg ctaccctggt tgaccccgtt
     5281 tatgaagcgc ttgaccaggc ttcgctcagc gtgatgggtt tcggggccgg ctacatcggc
     5341 agccagcccg aagtgaaaac gacggtcagc atttcccgga aagaaacgtt cgccaatgta
     5401 accgtgcttc cgtttcgcag gaatcccgtt acaggagcga tggaacggct cgtcagcttt
     5461 cagctccgta tttcggaaaa cggcagacgg gatgcacggt tcggcgttac gccgcagttt
     5521 gccaataaca gcgtgcttgc aacgggcacg tggtataaga tcggcgttgt gaatacgggc
     5581 gtgcagaaga tcgattacaa cttcctttcc aatatgggca tcgacatgtc cgcgctggac
     5641 ccgcgcaaca tcaggatcta cggcaacggg aaaggccagc tttcattcaa gaacagcgtt
     5701 gcccacgagg atgatctcca ggaatattcg atcgtggtgc agggtgaagc ggacggagtt
     5761 ttcgacacgc aggattatat tcttttctac ggcatttcac ccaactcatg gacattcgac
     5821 agcaccgaca tgcattttca tcatcacgtg catgattaca gcgacacgac ctattatttc
     5881 ctgaacaccg atctcggtcc gggaaaacgc gtcacgcagc gttcttcttc ctctcttaca
     5941 cctacagatg tagtgacgag cttcgatgat tacaagtttt tcgaatcgaa caatacgaac
     6001 ctcatcaaat ccgggcgcga atggtacggc gaggaattcg acatcatcaa ccagtacagc
     6061 tatcaattca attttccgaa tatcgatcct tcctcgcccg cgtgtgcaga ggtcgatctt
     6121 gtttcccgct gtgatgcgca gaattattat accgtcacgg cccagtccac gtcagcaacc
     6181 atcaccattt cgaatacaag cagctcctgc tattactgcc cgttcgccaa tcccgggaat
     6241 gcgaagctct gcttcaatcc cggcggtcct tcgatcctgg tgactgtaac gcggcaggca
     6301 aaccagtacg ccgttaccgg atggatgaac tgggtgcgtg tgaatgcgcg ccgtttcctg
     6361 acgatgacga acggacaaat gtttttccgt gattcgagaa gcgcgggtcc cggaaaagtt
     6421 tcacagttcg tgatggggaa ttacaatgcc agccagacca tctgggatgt gactgatttt
     6481 gcaaatatct accagcagaa cggaaccgtc ggcggcggaa attacacatg ggtggtcccg
     6541 acggattcac tgcatgagtt cgtttgcttt gacggcacgc aatacgagac accggtttat
     6601 cacgggcctg ttgcgaacca gaatctccat gcgctgcagc cggcagatct tattatcatt
     6661 tcgtatcctg attttcttcc gcaggcgctg gagctcgcgc agttccacgc gacgatgcag
     6721 aacttcagct atgtgatcgt cacaccgcag cagatctaca acgaattctc ttccggcgag
     6781 caggacgcga ctgcgctccg cgacttcgtg cgcatgctct actggcgtgc gggctccccg
     6841 tcgcaaacgc cgcgtttcct gctgctgttc ggcgacggct cttacgataa caagcaccgt
     6901 gttgccgcga attcaaattt cattcctacg ttccagaacc tgaattccgt cggcctcacg
     6961 gaatcgtata catccgatca gttctatacg ctgatggaca gcaacgaggg agaatgggac
     7021 gacagctccg acaacggcga ctgcgatatc ggtgtgggac gtttgccggt acggaacacc
     7081 acggaagcgg aggatatggt gaataaggtg aagcattaca tgacgatgcc gcagccggtg
     7141 tataatcttt cagcgtgctc ggcggccggg caatgttcga agggtggaga atggagaaac
     7201 tgtgtgacat tcatcggtga cgatgaagac aatgacattc acatgagcca ggcggaccag
     7261 ctggcgacat acgtttcgac caattacccg acctacaaca tcgacaagat ctatcttgat
     7321 gcctaccagc aggaacagac tcccggcggt gaacgttacc cggcggtgaa cgccgctttc
     7381 gaccagcgcg ttgacaaggg atgtcttatt ctcaattaca caggtcacgg cggagaagtg
     7441 ggacttgcgc atgaacgcat cctggaagtt tcgcagatca atgcgtggac gaatctctgc
     7501 aacatgccgc tgttcgttac cgctacctgc gagttcagcc ggttcgatga tcccgcgcgt
     7561 acttcggcgg gtgaatatgt actgcttaat tcgaacgggg gaggaatagg tctgctgacg
     7621 accgtgcggc ttgtttattc cacgccgaat ttcaacctca accagaaatt ctacgaatgt
     7681 gcgttcaacc cggtcaacgg ggaaatgccc tgcat
//
LOCUS       DASTSF010000047         2284 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_188342,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000047 DASTSF010000000
VERSION     DASTSF010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2284
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188342"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..278
                     /locus_tag="VFU15_01470"
     CDS             <1..278
                     /locus_tag="VFU15_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-dependent epimerase"
                     /protein_id="HEU4716464.1"
                     /translation="IRGAYNLAGFSFSPKEIAAAIQKHIPGFSISYKPDFRQQIADSW
                     PASIDDSEARKDWGWKENYTLDSMVEEMLTNIKKSLVKVETPHTPLP"
     gene            429..1337
                     /locus_tag="VFU15_01475"
     CDS             429..1337
                     /locus_tag="VFU15_01475"
                     /inference="COORDINATES: protein motif:HMM:NF019284.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716465.1"
                     /translation="MKKFTLIVLFIASVAIPAYSRAFVAASDTTPDSKNFVDPSGKRQ
                     GWWVITGKMRPDLKGYGPDAKVEEGNYKDSQKTGVWTEYYANGNKKSELTYVNNRPNG
                     HAVMYNDNGKKSEEGTWVGTRWTGPYTLYYDDGTPRQSFNYNQLGRRDGTQTYYHPNG
                     KVAITVDMKDGKETGWKKEYDENGNLIRETYFNDGVIDASKTKTYNVPPAPVSKAPEE
                     DKMKKTPPPPPDPNFKPNKGVFNGEGDWILYKNGQITMKGTFHKNRLVDGEERIYDGN
                     GMLVRIKLYKNGQYVGDGPLPTDANK"
     gene            1476..>2284
                     /locus_tag="VFU15_01480"
     CDS             1476..>2284
                     /locus_tag="VFU15_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008245837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEU4716466.1"
                     /translation="MAGALKVYNTLSRKKEEFIPLNPPYVGMYVCGPTVYSDVHLGNC
                     RTYISFDIMFRYLKFLGYKVRYVRNITDVGHLESDADEGDDKIGKKARLEKVEPMEIA
                     HRYSVGLWEVCAAFNLLPPSIEPTATGHIIEQIDMTKKILDNGYAYESKGSIYFDVEK
                     FSKENNYGILSGRTLEDQLSNTRELDGQEEKKGALDFALWKKASPEHILRWNSPWSEG
                     FPGWHLECSAMSRKYLGETFDIHGGGMDLIPTHHTNEIAQSVGCCGKEPVR"
BASE COUNT          662 a          585 c          581 g          456 t
ORIGIN      
        1 gcatccgggg tgcttataac ctcgccgggt tcagcttttc cccgaaagag attgcagcgg
       61 ccatccaaaa gcatatcccc gggttttcaa tcagttacaa accggatttc cgccagcaga
      121 tcgccgattc ctggcccgcg agcatcgatg attccgaagc ccggaaagac tggggctgga
      181 aagaaaacta cactctcgac agcatggtgg aagaaatgtt gacgaatatc aaaaaaagcc
      241 ttgtcaaagt tgaaactccg catactcccc ttccgtaaga agtaactaat accgctgtca
      301 gatatccttg gaggtttcgt cgaattatta actgaattga tcgaaaaccc gcaactttat
      361 tgagtttcag gcggtacatt taagtaaaat aatgttgaac agggtctaac ccacacacag
      421 gtgcccagat gaaaaaattt acgctgatag tactgttcat cgcttcggtc gcgatacccg
      481 cttacagcag ggcctttgtc gcagcttcgg ataccacacc agacagtaag aattttgtag
      541 atccctccgg aaaacgccag ggatggtggg tgatcacagg taaaatgcgc ccggacctta
      601 aaggttatgg tcctgatgcg aaagtcgagg aaggcaatta caaagacagc cagaaaacag
      661 gcgtttggac cgaatactac gccaacggca acaagaaaag cgagcttacg tacgtgaata
      721 accgtcccaa cggtcacgcc gtgatgtaca acgacaacgg aaaaaaatcc gaagaaggaa
      781 catgggtggg aacgcgctgg acaggtccgt acacgcttta ctacgatgac ggcacgccaa
      841 ggcagtcgtt caactataac cagctcgggc ggcgcgacgg aacgcagacg tattatcacc
      901 cgaacgggaa agtcgctatc accgtggata tgaaagacgg aaaagagacc ggctggaaaa
      961 aagaatacga tgagaatggc aacctgatcc gcgaaactta cttcaacgac ggcgttatcg
     1021 atgcttccaa gaccaaaacc tacaatgttc ctccggctcc cgtgtcaaaa gcgccggaag
     1081 aggacaaaat gaagaaaaca cctccgcctc ccccggatcc gaatttcaag cctaacaaag
     1141 gcgtattcaa cggcgaaggc gactggatcc tgtacaagaa cggccagatc acgatgaaag
     1201 gcacgttcca taaaaacagg ctggttgacg gtgaagagcg catctacgac ggaaacggaa
     1261 tgcttgtccg catcaagctc tacaaaaacg ggcagtatgt gggcgacggt cctctcccga
     1321 cggacgcgaa caaataggaa ttcgaaacga actgaagatc ccggaagaaa ttccgggatt
     1381 tttattttca cacggcctgc gttttccggg tcgcggatgc ttttcccgtg taattcccat
     1441 tctgctaact ttgttttccc gaaggaaaaa caactatggc aggcgctctg aaagtttaca
     1501 atacgttatc gcgaaagaag gaagaattca ttccgctgaa tcccccgtac gtgggtatgt
     1561 acgtgtgcgg acccacggtt tacagcgacg ttcatctcgg caactgccgc acgtatattt
     1621 cattcgacat catgttccgt tacctgaaat tcctcgggta caaagtgcgt tacgtgcgca
     1681 acatcaccga tgtcggccac ctcgaaagcg atgcagatga aggcgacgac aagatcggga
     1741 agaaagcgcg gctggaaaaa gtggagccga tggagatcgc gcaccggtac tcggtcgggt
     1801 tgtgggaagt ctgcgcggcg ttcaacctcc ttccgccttc catcgagccg acggctaccg
     1861 ggcacatcat cgagcagatc gacatgacga aaaaaattct cgacaacggt tatgcatacg
     1921 aatcgaaagg cagtatttat ttcgacgtcg agaaattttc gaaggaaaac aattacggca
     1981 ttctttccgg gcgcacgctc gaagaccagc tcagcaatac gcgcgagctc gacgggcagg
     2041 aagaaaaaaa aggcgcgctg gatttcgcac tctggaaaaa agcgagcccc gaacatattc
     2101 tccgctggaa ttcaccctgg agcgaaggct ttcccggctg gcacctcgaa tgttccgcga
     2161 tgagccggaa atacctcggc gaaacattcg acatccacgg cggcggaatg gatcttattc
     2221 ccacgcatca cacgaatgaa atcgcgcaaa gtgtcggctg ctgcggaaaa gagcccgtgc
     2281 gtta
//
LOCUS       DASTSF010000048         7000 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_857432,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000048 DASTSF010000000
VERSION     DASTSF010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 7000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7000
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_857432"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..433
                     /locus_tag="VFU15_01485"
     CDS             <1..433
                     /locus_tag="VFU15_01485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716467.1"
                     /translation="AAIGMQSEESNSIQKMISDLEAERANTSAPSTSILRSKERTPGT
                     QIGFIFNIPLAEQVAPGNYINATESADSGNFTAVFGTDMTDIFGNVLQEKWTYIPLVL
                     TVSNDVEQDLSQYKSNWSGYATATIYTYHESGFTPASQPTK"
     gene            454..1281
                     /locus_tag="VFU15_01490"
     CDS             454..1281
                     /locus_tag="VFU15_01490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716468.1"
                     /translation="MTTTSTLMKRRIAVTERKKAELDSLALQVLDAQQEVDQFQSIVD
                     GLTAKLATFQGYLSYAETNRTNALNNLNLVNQVVQNYLDLMNNSSTAFYGIADAGKWS
                     RTLSMQISRVMNKLIYSSEVINRLAALVIRKKALNPLISDELVSVLNTAGTDANNAVA
                     LTLVALQSVYASQASNTESEAAAALEYYEAMNVYEFIAGTNAVVIDASTQKPDCLQKL
                     LSDAYKNAVEYYNEMYTACQNISEDLNNAQQSLSEAQISLQSLQLGLAAGNAAALAS"
     gene            1346..2266
                     /locus_tag="VFU15_01495"
     CDS             1346..2266
                     /locus_tag="VFU15_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008469179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716469.1"
                     /translation="MENVYRKFYRACYMHTGGYIPAKPLNEPIYPGDFFQLRNGEMIL
                     LGNIFRAGVIDPGNAQLSSGVRLNGPLWNFSEDITKPYAGRGHGHGPVEGEFEFSRQI
                     IGFSSRGSFFFRGNEPESTRIANWNELQQAVIIRMTQTMFSFRQLYVVVDTASLADWT
                     LAISGSSKGELEIATDRENFNLLDLFGNSSSRTVQSKEIDYYHREAQRKPSFFRAKKL
                     VVQDEKIETFISDYIGRMTSRYEWANAFFEYDFQHDSSYSTITSQNTSATMLDMLQAN
                     ELNPNTALLYFKWADANLDDIEKMFVQDGD"
     gene            2256..3611
                     /locus_tag="VFU15_01500"
     CDS             2256..3611
                     /locus_tag="VFU15_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007096235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU4716470.1"
                     /translation="METELSIAVLHNEINWLQEVISQVIRSYLLQEGHEKNWQDILPP
                     DISASESPYALKVLEWQPGVFGRIALALAIAPHLRPEALDVFFGKNQLYDRGFTEFGG
                     VVDKNHSGFLPTAQTLYFLLTAVNPELRPEVMKLLSKENVLIRDEVIQLSEVDQNLPP
                     LNAMLTMSSYWFEYFITGVHPPEESNPAFPAQRITTGLEWEDVVLDDNVMEQVEEIQA
                     WLQHSSTLMNEWGLKKMLKPGYRALFYGPPGTGKTLTATLLGKMSGRDVYRIDLSMIV
                     SKYIGETEKNLARVFAIARNKHWILFFDEADALFGKRTTTNSSNDRHANQQTAYLLQL
                     IEDYPGVVILASNLNANIDDAFTRRFQSMIHFAMPSAEERLQLWTNAFSGTCTLHPDI
                     DLRKIAEDHQLSGGSIINVLRTCALTAVRTSDPVVTQKNLLAGIRKEFRKENKTLIIS
                     S"
     gene            3620..4177
                     /locus_tag="VFU15_01505"
     CDS             3620..4177
                     /locus_tag="VFU15_01505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716471.1"
                     /translation="MSTHLLHTEKKHPHPETNVQLAIGEWRELFTESEYKLIIAESLN
                     ETVVTRGFHTILNGYLITKRRVCLVLHGDREKAQAMLDVFYRNVKKQLLRRLHAKPAE
                     MQKEIIHRLALEGHEGHRHLFREFPLYNYVLVRLITGRPAGVLYYSPHVERLKERIRN
                     YPYCSAIDYSGAKGPVIVTTVNLKN"
     gene            4351..5610
                     /locus_tag="VFU15_01510"
     CDS             4351..5610
                     /locus_tag="VFU15_01510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716472.1"
                     /translation="MKKGTGNGVVQLGKYNKKKKKKDGKSKVLHQHTLKPSVAATMKV
                     SPSFSKALTSGEHEEDIFNSMKTSIETQITKSGSLQKEMEDDEDIDFIEENTAQLSIV
                     LLQAKNTLEEIKKRPYQRFRSKDKVRAAYVNKLHELNNRQRILINELKRQFPTHKGGF
                     GAKGRAHAMAVEIDPTDVPMTKVEDNSFVSNIKLDKVALNAKVYKEELKVGKEAAGPI
                     KIMGKDATAYKKFSKKKVLADVEKPMSPVESSQKVTLKDAPAGKARGQGQYANMNNTN
                     AAGYAWLINAPGMNSQRWEWLHVRGAGLGGATDSTNLVTGTRDINTQMIPFESNIRQL
                     GAIVKKYPNKYKEFKVDWAVAKQYEGAAHAFSEISIDWKLINKSGADATSGHVAFNPM
                     HTASNISKDEVNLLENKLKEKRDSVDH"
     gene            5770..6354
                     /locus_tag="VFU15_01515"
     CDS             5770..6354
                     /locus_tag="VFU15_01515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716473.1"
                     /translation="MEKEAPEGEEAESDEPVQLAKNKKRWTPQQREQSRKFWEKKGKK
                     LDRRFVGDHITRTNEEKKNKEELPVIVKGDVSGHLNEALTLIVLQGSTTPGQHQDLIC
                     NLSSGANLRFSRNQSEDTAQYGAEEGRMPHIASYPPQGKQTVRSALIAFRQAHDDMRC
                     YFNGDCQSFASKIVEKLAGTRTGYGASAEEESFM"
     gene            complement(6347..>7000)
                     /locus_tag="VFU15_01520"
     CDS             complement(6347..>7000)
                     /locus_tag="VFU15_01520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716474.1"
                     /translation="ETTLEKVDHSLFLFIDENPFGEVPFAERLYVMTFRMREKTIPCA
                     HFFSDCFPEGGGFFSLGFSCTPCRYLFVPGIHHVTGERAGLTKMADALLLRKEFDKLE
                     RQATAAGFPFFQFRLPVRTVGHYTLRDRRRAVVGEVERASALVRGVSCRCGQLPSCRM
                     RGKCGVAWHNRVLDQECITGVIITLPVTAGTLLSCFMRFLFIDVSLFYWFAPCRQVT"
BASE COUNT         1942 a         1938 c         1818 g         1302 t
ORIGIN      
        1 cgccgccatc gggatgcaaa gcgaggaatc gaacagcatc cagaaaatga tctccgacct
       61 cgaggcagag cgtgcaaaca catcggcgcc ttcaacctct atcctcaggt cgaaggagcg
      121 gacaccgggc acgcagatcg ggttcatctt caacatcccg cttgcagaac aggtcgcacc
      181 cggaaattac atcaacgcca ccgaaagcgc ggactccggc aactttacag ctgttttcgg
      241 aacagacatg accgacattt tcggcaatgt gctgcaggaa aaatggacat acattcccct
      301 cgttcttacg gtaagcaatg acgttgagca ggacctttcc cagtacaaga gcaactggtc
      361 aggttatgca acggcgacta tttacacgta ccatgaatcc ggcttcacac ccgcttccca
      421 gccaaccaaa taacgaacct aaagcattga aacatgacaa ctacaagcac acttatgaaa
      481 cggcggatcg ccgtaaccga acgcaagaag gcagaactcg attcgcttgc gctgcaggta
      541 ctcgacgcac agcaggaagt cgatcagttc cagtctatcg ttgacgggct caccgcgaag
      601 ctggcaacat tccagggata tctttcctat gcagaaacga accgcacgaa tgcgctgaac
      661 aacctgaacc tcgtcaacca ggtggtgcag aactacctcg acctgatgaa caactcttcc
      721 accgcgttct acgggatcgc cgatgcgggc aaatggtcgc gcacactttc gatgcagatc
      781 agccgtgtga tgaacaagct gatctactcc tcggaagtca tcaaccgcct tgcagcgctc
      841 gtcattcgta aaaaagcgct caacccgctc atctctgatg agctggtcag cgtgctgaac
      901 accgccggga ccgatgcgaa caacgccgtc gcgcttacgc tcgtggcgct gcagtcggtg
      961 tatgcctcgc aggcgtccaa cacggaatca gaagcggccg cagcgctcga atattatgag
     1021 gccatgaacg tgtatgaatt catcgccggc acgaatgctg tggtcatcga cgcgtctacg
     1081 caaaaacctg actgtctgca gaagctgctc tccgacgcgt acaagaacgc ggtggagtac
     1141 tacaacgaga tgtatacggc ctgccagaac atttcagaag atctgaacaa cgcgcagcag
     1201 tcgctgagcg aggcgcagat ctcactgcag tcgctccagc tcggcctcgc cgccggcaac
     1261 gcagcagcgc ttgcatcctg accgttcgaa cgaacatcca ccgtcctgca tcatggcggg
     1321 acggtggata ttttaccagt aagcaatgga aaacgtctac cgaaaattct accgcgcgtg
     1381 ctacatgcac accgggggat acatccctgc gaagccgctc aacgaaccga tctacccggg
     1441 cgatttcttc cagctgcgca acggcgagat gatccttctc ggaaatattt tccgtgcggg
     1501 cgtcatcgat cccgggaacg cacagctttc aagcggggtg cgtctcaacg gtcctttgtg
     1561 gaacttcagc gaggacatca ctaaaccgta cgcgggtcgc ggacacgggc acggacctgt
     1621 ggaaggcgaa tttgaattca gcaggcagat catcggcttc tcgtcgcggg gaagtttttt
     1681 cttcagggga aacgaaccgg aatcgacgag gatcgccaac tggaatgagc tccagcaggc
     1741 ggtcatcata cgcatgacgc agaccatgtt ctcgttccgg cagctctatg tcgtcgtcga
     1801 tacggcctcg ctggccgact ggacactggc gatctcgggc tcttcgaaag gtgagctgga
     1861 aatcgctacg gacagggaaa acttcaatct cctcgatctt ttcggcaaca gctcctcgcg
     1921 gacggtccag tcgaaagaga tcgattatta tcaccgggaa gcacagcgga aaccttcttt
     1981 cttccgtgca aagaagctcg tcgttcagga tgaaaagatc gaaacgttca tcagcgatta
     2041 catcgggcgc atgacgagca ggtacgagtg ggccaacgcg tttttcgaat acgattttca
     2101 gcacgactct tcctactcca cgatcacatc gcagaacaca tcggctacga tgctcgacat
     2161 gctgcaggcg aacgagctga acccgaacac ggcgttgctc tatttcaaat gggcggatgc
     2221 caatctcgac gacatcgaaa aaatgttcgt acaggatgga gactgaactt tccatagcgg
     2281 tattgcataa cgagatcaac tggctgcagg aagtgatcag ccaggtgatc cgttcatacc
     2341 tgctgcagga aggtcatgaa aaaaactggc aggatattct tccgccggat atttccgcgt
     2401 cggaatctcc ctatgcgctg aaagtgctgg agtggcaacc gggggttttc ggccggatcg
     2461 cactcgcgct ggcaatagcg ccgcacctgc gcccggaagc gctcgacgtc tttttcggga
     2521 agaaccagct gtacgaccgt ggcttcacgg aattcggagg cgtggtcgat aaaaatcaca
     2581 gcggcttcct gcctaccgcg cagacattgt atttcctgct gaccgcggtg aacccggagc
     2641 tccgcccgga agtgatgaag ctgctttcga aagagaacgt gctcatccgc gatgaagtga
     2701 ttcagctcag cgaagtcgac cagaacctgc ccccgctcaa cgccatgctg acgatgagca
     2761 gctactggtt cgaatatttc atcacaggcg ttcatccgcc cgaggaaagc aatcctgcat
     2821 tccccgcgca gcgcattaca acgggactcg aatgggagga cgtcgttctc gacgacaacg
     2881 tgatggaaca ggtggaagag atccaggcgt ggctgcagca cagctccacg ctgatgaatg
     2941 aatgggggct gaaaaaaatg ctgaaacccg gctaccgcgc attgttctac ggtccgccgg
     3001 gaacgggaaa aacactgacg gcaacactgc tcggaaaaat gtccgggcgc gacgtgtacc
     3061 gcatcgacct gtccatgatc gtttcgaaat acatcggtga aacggaaaaa aatctcgcac
     3121 gcgtattcgc gatcgcccgc aacaagcact ggatactttt cttcgatgaa gccgatgcgc
     3181 ttttcgggaa gcggaccaca acgaattctt cgaacgaccg tcacgccaac cagcagaccg
     3241 cttacctgct ccagctgatc gaggattacc cgggagtcgt cattcttgct tcgaacctga
     3301 acgcgaacat agacgatgca ttcacccgcc gcttccagtc gatgatccac ttcgcgatgc
     3361 cgtcggcgga agaacggctg cagctttgga cgaatgcatt ttccggcacc tgcacgctgc
     3421 atcccgatat cgacctgcgc aagatcgccg aggatcacca gctttcaggc gggtcgatca
     3481 tcaatgtcct gcgcacctgc gcactcacgg ccgtgcggac aagcgatccc gttgtcacgc
     3541 agaaaaatct tctcgccggg atccggaaag aattccggaa agaaaacaaa acgcttatca
     3601 tcagcagtta accgatcgca tgagcacaca tcttcttcat acggaaaaaa aacatccgca
     3661 cccggagacg aacgttcagc tcgctatcgg ggaatggaga gaactgttca ctgaatccga
     3721 atacaagctc attatcgcgg aatcactgaa tgaaactgtg gtaacgagag gattccacac
     3781 gatcctgaac gggtacctca tcaccaaacg cagggtgtgc ctcgtcctgc acggagaccg
     3841 ggagaaagcg caggctatgc tcgacgtttt ttaccgcaac gtaaaaaaac agctgctccg
     3901 ccgtttacat gcgaaaccgg ccgaaatgca gaaagagatc atccacaggc tggcactgga
     3961 aggccatgaa ggacaccgcc acctcttccg cgaatttccc ttatacaact acgtgctcgt
     4021 acggctcatc acggggcgtc cggcaggcgt gctttattac agtccgcacg tcgagcgcct
     4081 gaaggaacgc atccgtaatt acccgtactg ttcggctatt gattattcgg gagcaaaagg
     4141 accggtaatc gttacaactg taaacctcaa aaattaggga cgctttccgg tcactgctca
     4201 ttaacggatc aaccattgac aatgatgttc aatcctgtac aaacggcgca aaccacatct
     4261 tccggcccgg tcgcggccgc gcgatcacgt gggactgcaa ctgcaggagc agcgctgaaa
     4321 gataaccgcc ccgcaccggt cctgcaactg atgaaaaaag ggacgggcaa cggtgtcgtg
     4381 caattgggta agtacaacaa gaagaaaaaa aagaaggacg ggaaaagcaa agtcctccac
     4441 cagcatacgc tgaaaccatc ggttgcagcg acaatgaaag tgtcgccttc cttttcgaaa
     4501 gcgctcacgt cgggagagca tgaagaagat atcttcaatt ctatgaagac cagcatcgaa
     4561 acgcaaatca caaaatccgg gagtcttcag aaggaaatgg aagatgacga ggatatcgat
     4621 ttcatcgagg agaatacggc gcagttgagc attgttttac tacaggcgaa aaatacgctc
     4681 gaagaaataa agaaaagacc ttaccagaga ttccgcagca aagacaaggt aagagctgcg
     4741 tatgtcaata aactccatga gctcaataac aggcagcgga tactcattaa cgaactcaaa
     4801 agacagtttc ctacgcacaa aggcggcttc ggtgccaaag gacgtgcgca tgccatggct
     4861 gtcgagatcg accccaccga tgtgccgatg acaaaagtgg aggacaattc attcgtcagc
     4921 aacatcaaac tggacaaggt cgccctgaat gcgaaagtct acaaggaaga actcaaagtc
     4981 gggaaagaag ccgcaggacc gatcaagatt atggggaagg acgcaactgc ttacaaaaag
     5041 ttttctaaaa agaaagttct tgccgacgtg gaaaaaccga tgtcccccgt tgaatcgtcg
     5101 cagaaagtca ctttgaagga tgcgcccgca ggcaaagcgc gaggccaggg tcaatatgca
     5161 aatatgaaca acaccaacgc tgcaggctac gcgtggctca tcaacgctcc cggcatgaat
     5221 agccaacgat gggaatggct acacgtgcgc ggcgcaggat tgggaggcgc tacggattcg
     5281 accaaccttg ttaccggaac acgtgacatc aacacgcaga tgatcccgtt cgaatcaaat
     5341 atccggcagt tgggggcgat cgttaaaaag tatcccaaca aatacaaaga gtttaaggtc
     5401 gactgggcag tggcgaaaca atacgaaggc gccgctcacg cgttcagcga aatctcgatc
     5461 gactggaaac tgatcaataa atcaggcgcc gacgccacat ccggtcatgt agccttcaac
     5521 ccgatgcaca cggcttcgaa tatttcaaaa gatgaagtca acctgctgga gaataagttg
     5581 aaagaaaaac gcgatagcgt agaccactaa cgaacgacaa ccattcgcca caactgaaaa
     5641 cgcatgcacg ccagccggac atccttatca ccatcacatg accgcaaaca ggttccggct
     5701 gtgcatgcag ccgggagcgg cgcgggtttc tttctccggg acaatcgccc tgcgtccgta
     5761 ttccagcgca tggaaaagga agcgcccgaa ggagaagaag ccgagtcgga cgaacccgtt
     5821 cagcttgcga aaaacaaaaa gcgatggaca ccgcagcagc gggagcagag ccggaaattc
     5881 tgggagaaga aaggcaaaaa actcgaccgc aggttcgtcg gcgatcatat tacccggacc
     5941 aacgaagaaa aaaagaacaa ggaagagctg ccggtaatag tcaaaggcga tgtatccggt
     6001 cacctgaatg aagcgctgac gctgatcgtc ctgcagggct cgacgacgcc ggggcagcac
     6061 caggatctca tctgcaatct cagctccggg gcgaatctcc gcttttcacg gaaccagtcg
     6121 gaagacacgg cccagtatgg cgcggaagag ggacgaatgc cccatatcgc atcgtacccg
     6181 ccgcagggaa agcaaaccgt acgcagcgcg ctgatcgcat tccggcaggc acatgacgac
     6241 atgcggtgct atttcaacgg cgactgccag tcgttcgcca gcaaaattgt cgaaaagctg
     6301 gcgggcacgc ggaccggata tggcgcatcg gcagaagaag agagtttcat gtgacctggc
     6361 ggcatggcgc aaaccaataa aaaagcgaaa catcaatgaa cagaaaacgc ataaagcacg
     6421 aaagcaaagt tccggccgtc acaggtaatg tgataataac acccgttata cactcctgat
     6481 ccaaaactct gttatgccag gcaaccccgc attttccacg cattctgcag gaaggcagct
     6541 gaccacagcg gcaggaaacg cctcgcacca gcgcggaagc tctttctacc tcaccgacaa
     6601 ccgcccttcg ccggtcacgc agcgtgtaat ggccgacggt gcggaccggc aaccggaact
     6661 gaaagaaagg aaaacctgca gcagttgcct gcctttccag tttgtcaaat tctttacgga
     6721 gaaggagcgc gtcagccatc ttggtcaaac cggctcgctc accggtcacg tgatgtatgc
     6781 ccggaacgaa cagatagcga cacggcgtgc acgaaaatcc aaggctaaaa aagccaccgc
     6841 cctcaggaaa acaatccgaa aaaaaatgag cgcacgggat cgttttttca cgcatacgga
     6901 aggtcattac gtaaagacgc tcagcaaacg gaacctcgcc aaaagggttt tcatcgatga
     6961 aaagaaaaag agaatggtcc accttctcca gggtcgtctc
//
LOCUS       DASTSF010000049         6545 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_188284,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000049 DASTSF010000000
VERSION     DASTSF010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 6545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6545
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188284"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..4021
                     /gene="glgP"
                     /locus_tag="VFU15_01525"
     CDS             <1..4021
                     /gene="glgP"
                     /locus_tag="VFU15_01525"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HEU4716475.1"
                     /translation="TGEHPEFREDKNLFVSWRAFAEARELKIRIGRWKIPGNPIAILI
                     DYTGLFMEKDTIFRDLWLQFKVDSLTGQWDYIEPALFGYAAGKVIECFYHHYLTLSDK
                     IVAQFHEWMTGAGLLYVESHVPQIGTVFTTHATVTGRTIAGNGLPLYGKLESYDGDRE
                     AKRFNVISKHSLEKTAAHVADCFTTVSEITATECRQLLQKEPDVITPNGFEAAIVPDP
                     LLFRERRAAARKKLLGVAAALTGKELNDDTLLVLKSGRYEFRNKGVDIFIDAMGELNR
                     EGNLPKNIVAFIFIPAHHVGPREDLLRRINNENIIPESRTLTHHLQGADSDPVLQRLR
                     LRELDNKPADPISVIFTPVYLDGHDGIFNLSYYELLTGFDISVFPSYYEPWGYTPLES
                     LAFHIPAVTTTLTGFGMLLKESSDGAYVIARNDSNDSFVAEKIASILREYAGKNENEI
                     RSARENAYRLSKKALWKNLIVYYKKAYSIALQKTHEREEKFTHPPHVQEWDTCPAPSQ
                     LPPAAKPMWRKVFVKSEFPSSLLPLQELAGNLWWTWNSNAEELFSSVDPLLWETCGQN
                     PVEMLNNLSHKVISQLSEDESFLRRLAAVTNDFRAYMKAAPGGPRIAYFCMEYGLHNS
                     LKLYSGGLGILAGDYLKQASDSCADITGIGLLYRNGYFRQKIAASGEQVVTDDHQKFT
                     ALPLHPVINAEGDWLTINIAFPARTLYARAWRIDVGRTKLYLLDTDIHENIEEDRQIT
                     SHLYGGDHENRLRQEIVLGIGGVRLLYILGIKPDIFHYNEGHSAFAGIERLYHLVLDE
                     KLSFGESVEVVKASSLFTTHTPIAAGHDTFSEEMLRAYFSHYAQAFNISWNDLLNLGK
                     TGRNSQELFSMSYLASKLAGGINGVSAVHEQVTRKMFNVLWPAYLPEELHIGHVTNGI
                     HLSTWMAPEWKKLCGEDALTDDHVAASTLSGLPPARIWETHLQLKQQLISAINSKYGN
                     AITGEKKDRSLPGSEVLLIGFARRFVPYKRSDLLFRDLDKLSRILNREGEPVAILFSG
                     KAHPDDVAGQTLLKKVKEISVLPQFEGKILFLENYDMAAARMLVSGVDVWLNTPVAGM
                     EASGTSGMKAGINGVLNLSVCDGWWAEAFDGELGWCIGNGNSGVADSDENDAASLYNL
                     LDGEIIPLFFRRNAQNIPVSWVERMKKSFAHIIPAYSMQRALQQYGSAYYAPLLTRFR
                     FLGSNEFENAAKLAAWKELIRKSWNTIEVQSVKVHNATQKPFPVGEKMEMEVILSGGT
                     VPPGNIGVEIVFTDAKQGNRRIVMKRELDPSEPVPGKLCYSAAVAMENPGVYQYSFRL
                     FPKNTLWQEPELSVFARWI"
     gene            4162..5409
                     /locus_tag="VFU15_01530"
     CDS             4162..5409
                     /locus_tag="VFU15_01530"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006987870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HEU4716476.1"
                     /translation="MDTPAKRLVILGAGTAGTMMANRLEKELEKGKWKITIVDECETH
                     YYQPGFLFLPFDAYTPGEITRPVSEFIPAGVNLVRKKINKIVPAENHVLLDDGECLPY
                     DLLIIATGSKIAPEEVEGMNGKEWRNSIFDFYTFEGALALRDKLKNWEGGKLVVHITE
                     MPIKCPVAPLEFAFLAESFFRHKKMRDKVEITFVTPLSGAFTKPKATAELGHLLAEKN
                     IHIVSDFAIEKVDNEHKKIIDYGDREVPFDLLVTVPTNKGDEFIARSGLGDELNFVPV
                     NRATLQSDAWKNIFVIGDASNVPASKAGSVAHFESGILSENILHYINGEPLKEEFDGH
                     ANCFVETGDGKALLVDFNYTHEPVEGTFPFPGIGPFRLLHESQMNHFGKLAFRWIYWN
                     MMLKGKHIPFVSAEMQESGKHFE"
     gene            5431..5739
                     /locus_tag="VFU15_01535"
     CDS             5431..5739
                     /locus_tag="VFU15_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014792663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TusE/DsrC/DsvC family sulfur relay protein"
                     /protein_id="HEU4716477.1"
                     /translation="MEKTISGKTVSVNEEGYLTDFSQWNRDIAGEIAREEQIVLSERH
                     WEVIAYLQEQYKKKIPMSIRKIRNSGVIDIKEFYALFPKGPLKTASRIAGLPKPVSCI
                     "
     gene            5751..6233
                     /locus_tag="VFU15_01540"
     CDS             5751..6233
                     /locus_tag="VFU15_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010424275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HEU4716478.1"
                     /translation="MSENNNGLKKMLIILSKATLENVYAAFILANGARMEGIEAEMFF
                     TFFGLEAIHKKKLEHLHIATVGNPAMHIPTLLGGLPGMESIATSMMKKEMERIDMPGV
                     HEFLDILKASGVKMWGCKLAMDMFHYRKEDLYDDIDGVLTVGDFYKQAQGEGSHILFV
                     "
BASE COUNT         1743 a         1627 c         1722 g         1453 t
ORIGIN      
        1 aacaggcgaa cacccggaat tccgggagga taaaaatctt tttgtctcgt ggcgtgcctt
       61 tgcggaagcg cgtgaactga aaatccgcat cggccggtgg aagatcccgg gaaacccgat
      121 cgccatcctc atcgattaca cggggctttt catggaaaag gacacgatct tccgcgatct
      181 gtggctgcaa ttcaaggtcg actcgctgac cggccaatgg gattatatcg aaccggcgct
      241 gttcggttat gctgcgggga aagtgatcga atgtttctat caccattacc ttacgctgag
      301 cgataaaatc gtcgcgcagt tccatgaatg gatgacaggt gcaggattgc tttacgtcga
      361 atcgcatgtc ccgcagatcg gcacggtctt cacgacacat gccaccgtca ccgggagaac
      421 gatcgccggg aacgggcttc cgctgtatgg aaaactggaa agctacgatg gcgaccggga
      481 agcgaaacgg ttcaatgtca tttcaaaaca ctcgctggag aaaacagcgg cgcatgtggc
      541 cgattgcttt accacggtga gcgagatcac cgcgacggaa tgcaggcaac tgctccagaa
      601 ggaaccggat gtgatcacac cgaacggttt cgaagccgct atcgttcccg acccgctcct
      661 gttcagggaa aggcgggcgg cggcgagaaa aaaactgctc ggtgtggcgg cagcgcttac
      721 cggtaaagaa ctgaacgacg ataccctgct cgtcctgaaa agcgggcgtt acgaattccg
      781 gaacaaaggc gtcgatattt tcatcgatgc gatgggtgag ctgaaccgtg aagggaatct
      841 cccgaaaaac atcgtcgctt tcatcttcat tccggcgcat catgtcggtc cgcgcgaaga
      901 tcttctccgg aggataaaca atgaaaatat aatcccggaa agccgcacac tcacgcatca
      961 cctgcagggc gcggacagcg acccggtcct gcagcgtctc cgtttgcgcg agctggacaa
     1021 taaacctgcg gatcccatca gtgtgatttt cacacccgtt tatcttgacg gtcatgacgg
     1081 tattttcaac cttagctatt acgagctgct taccggcttc gacatttccg tctttccttc
     1141 ctattacgaa ccctggggat acacgccgct ggaaagcctc gcttttcaca ttcctgcggt
     1201 aaccactacc ctgaccggtt tcggcatgct gcttaaagaa agctcggacg gcgcttatgt
     1261 gatcgcacgg aatgacagca acgattcctt cgtcgcggaa aagatcgcgt cgatacttcg
     1321 ggaatatgcc ggcaaaaatg aaaacgaaat aaggtcggcg cgtgaaaacg cttatcgtct
     1381 ttccaaaaaa gcgttatgga aaaacctgat cgtttattat aaaaaagcat acagcatcgc
     1441 cctgcagaaa acgcatgagc gggaggaaaa attcacgcat cccccgcatg tgcaggaatg
     1501 ggatacatgt cccgctccat cgcagcttcc ccccgcggcc aaacccatgt ggcgaaaagt
     1561 tttcgtcaaa tccgaatttc cttcctccct cctgcccttg caggagctgg caggaaacct
     1621 ctggtggaca tggaacagca acgcggaaga actgttttct tccgtcgatc cattgctgtg
     1681 ggaaacgtgc gggcaaaatc cggtggaaat gctgaacaac ctttcgcaca aagtcatcag
     1741 ccagctttcg gaagacgaat ctttccttcg caggcttgcg gccgtaacaa atgatttccg
     1801 cgcgtatatg aaggcagcgc cgggaggtcc gcgcattgcc tatttctgca tggaatacgg
     1861 gctgcacaac agcctgaaat tatattccgg cggactcggc atacttgccg gcgattatct
     1921 gaaacaggcg agcgattcct gcgcggatat cacgggaatc ggtttgctgt acaggaacgg
     1981 ttatttccgt cagaaaatag ccgccagcgg agaacaggtg gtcacggacg accaccagaa
     2041 attcacggcg cttccgttac atcccgtcat caatgcggaa ggcgactggc ttaccatcaa
     2101 tatcgcattt cccgcgcgca cgctctatgc acgcgcctgg aggatcgatg tcggaagaac
     2161 aaagctctac ctgctcgaca ccgacattca cgagaatatc gaagaagacc ggcagatcac
     2221 gtcgcacctg tacgggggcg atcacgagaa ccgcctcagg caggagatcg tgctgggcat
     2281 cggcggcgtg cgcctgctgt atattctcgg gataaaaccg gatattttcc attacaacga
     2341 agggcattcc gcatttgccg ggatagaacg gttgtaccat ctcgtgctgg atgaaaaact
     2401 ttctttcggg gaatccgtgg aagtggtgaa ggcttcctct cttttcacca cacatacgcc
     2461 gatagccgcc ggtcatgata cgttcagcga agaaatgctg cgcgcttatt tttcacatta
     2521 tgcccaggcg ttcaatattt catggaacga cctgctgaac ctcgggaaga cgggacgcaa
     2581 cagccaggaa cttttctcga tgagctacct cgcttccaag cttgccggcg ggatcaatgg
     2641 cgtcagcgcc gtccatgagc aggtgacgag aaaaatgttc aacgtgctct ggcccgctta
     2701 tcttcccgaa gagcttcaca tcggccatgt cacaaacggc attcatcttt ccacgtggat
     2761 ggccccggaa tggaaaaagt tatgcgggga agatgcattg acggatgatc acgtcgcagc
     2821 atccacgctt tcgggccttc cgccggcgcg gatttgggaa acgcacctgc agctcaagca
     2881 gcagttgatc agtgctataa attcgaaata cggaaacgcg ataaccggtg aaaaaaaaga
     2941 ccgttccctc ccgggcagcg aagttctgct catcggcttt gcgcggcgct ttgttccgta
     3001 taaacggtcc gatcttttat tccgcgatct cgacaaactt tcccgcatac ttaaccggga
     3061 aggagaacct gttgcgatcc tgttctccgg caaagcgcac ccggatgatg ttgccgggca
     3121 aacattgctc aaaaaagtca aggagatttc cgtgcttcct caattcgaag gaaagatact
     3181 tttccttgaa aattacgaca tggctgccgc acgcatgctt gtcagcggtg ttgatgtatg
     3241 gctgaacacg ccggtcgcag gaatggaagc ctcgggaacc agcggaatga aagcaggtat
     3301 aaacggcgtg ctcaacctca gcgtttgcga cggatggtgg gcggaagcat tcgacgggga
     3361 acttggctgg tgtattggca atgggaattc cggcgtcgct gattcggatg aaaatgacgc
     3421 cgcgtccctg tataatcttc tcgacggcga aattattccc ctgttcttcc ggaggaatgc
     3481 gcagaatatc cccgtctcct gggtagaacg aatgaaaaaa tcgtttgctc acatcatccc
     3541 ggcttattcc atgcaaagag cattgcagca atatggatcg gcatattacg cgccgctgct
     3601 cacccggttc cgcttccttg gcagcaatga atttgaaaac gcagcgaaac tcgccgcgtg
     3661 gaaggaactg atacgtaaat cgtggaacac gatcgaggtt caatcggtga aggtccataa
     3721 tgcgacacag aaaccatttc cggtcggaga aaaaatggaa atggaagtca tcctttccgg
     3781 tggaactgtt ccgccgggaa acatcggtgt cgagattgta ttcacggatg cgaaacaggg
     3841 aaacaggaga atcgtgatga aacgggaact cgacccgtcc gaaccggtgc cgggtaaact
     3901 ctgttactcc gccgctgttg ccatggaaaa tcccggcgtt tatcaataca gtttccggct
     3961 gttcccgaaa aatacgttgt ggcaggaacc ggaactttcc gtgttcgccc ggtggatatg
     4021 aaatgctacc ggaagacgat tttcccgggc tgaagtaaat cacaatgtgt cgtgagactt
     4081 gtcattagct ttttttctga agcgggacat ttttgcagcg ttatctccgc cccttcattt
     4141 taaaagttca gttacaggaa cgtggacaca ccggcaaaac gattggtcat actcggagca
     4201 ggcactgcag gaacgatgat ggcgaaccgg cttgaaaaag agctggaaaa agggaaatgg
     4261 aaaatcacca tcgtggatga atgcgagacg cattattacc agcccggttt cctgttcctt
     4321 ccgtttgatg cctatactcc gggagaaatt acgagaccgg tcagcgaatt tattcctgcc
     4381 ggcgtgaacc ttgtccggaa aaaaattaac aagatcgttc ccgcggaaaa ccatgtactt
     4441 ctggacgatg gagaatgcct cccgtatgac ctgcttatca tagccactgg ttcgaagatc
     4501 gcccctgaag aagtggaagg catgaacgga aaggaatggc gcaacagcat ctttgatttc
     4561 tatacgttcg aaggtgcgct tgctttgcgg gataagctga aaaactggga aggcggaaag
     4621 ctcgtcgtgc acatcacgga aatgcccatc aaatgcccgg ttgcgccgct ggagttcgca
     4681 tttcttgccg agtctttttt caggcataag aaaatgcggg ataaggtgga gatcacgttc
     4741 gtcacgccac tgagcggagc gtttacgaaa cccaaagcaa cggcagagct cggtcacctg
     4801 ctggcggaga aaaacataca catcgtaagt gattttgcca ttgaaaaagt agacaatgag
     4861 cataaaaaga tcattgatta cggcgaccgg gaagtgccgt tcgatctcct ggtaacagtt
     4921 cccaccaata aaggcgatga gttcattgca cgttccgggc tcggcgatga gctgaacttt
     4981 gtccctgtaa acagggctac gctgcaatcg gacgcgtgga aaaatatttt cgtgatcggc
     5041 gatgcgagca atgttcctgc ttcgaaagcg ggttccgtgg cgcatttcga atccgggata
     5101 ctttcagaaa acatcctgca ttacatcaat ggagaaccgt tgaaagagga atttgacggg
     5161 catgccaatt gctttgtcga aaccggcgac gggaaagccc tgctggtcga tttcaattac
     5221 acgcacgaac ccgtggaagg aacatttcct tttccgggca tcgggccatt ccgtctgctg
     5281 catgaaagcc agatgaatca tttcgggaaa cttgctttcc gctggatcta ctggaacatg
     5341 atgctgaaag gaaagcacat cccgttcgta tccgcagaaa tgcaggagag cggaaaacat
     5401 tttgagtaaa ccagctgaac atattgaacc atggaaaaaa caataagcgg gaaaacggtt
     5461 tcggttaacg aagaaggtta cctcactgat ttttcacaat ggaacaggga tatcgccggt
     5521 gaaattgccc gcgaagagca gatcgttctc tccgaacgtc actgggaagt gatcgcttac
     5581 ctgcaggaac aatacaaaaa gaaaataccg atgagcatcc gcaagatccg gaacagcggc
     5641 gtgatcgaca taaaggaatt ttatgcgctg ttcccgaaag gtccgctcaa aacagcatcc
     5701 aggatcgccg gtcttcccaa gccggtaagt tgcatttaaa accgaacgtc atgagcgaaa
     5761 acaacaatgg attaaaaaaa atgctgatca ttctttccaa agcgacattg gaaaatgtgt
     5821 acgccgcatt tatactggcg aacggtgcgc ggatggaagg tatagaagct gaaatgttct
     5881 ttaccttctt cggcctggaa gccattcaca aaaagaagct ggagcacctg cacattgcaa
     5941 cggtcggcaa tccagcgatg cacattccta cgctcctggg cggtttgccg ggaatggaaa
     6001 gtattgccac gtccatgatg aaaaaagaaa tggaacggat cgatatgccg ggcgtgcatg
     6061 aattcctcga tatcctgaag gcttccggtg tgaagatgtg gggctgcaag ctggcaatgg
     6121 atatgtttca ttacaggaag gaagatctgt acgatgacat cgacggcgtt ctgaccgttg
     6181 gcgactttta taaacaggcg cagggagaag gttcgcacat cctgttcgtg tgaacggcta
     6241 attcagctat aaggaagctg gaaagcaaaa ttcaacttaa tcctatcgtt atgaaacgag
     6301 atccgcttac gtacaaatag caggcggagg agtccgttat tgcttatcaa gggcaggttc
     6361 gcgcaaaaga aaaaaaacgg caggcttccg gagacgtttt tgcacggcag atcaccagcg
     6421 gataaggatg atcgttttcg gtcctttccc ggcaaaattc ccgtacttta caggaacaac
     6481 agcagaaaaa atggaaaaag aaaagaagca cctgatgcac aagcttcacg tggagctggt
     6541 gaaat
//
LOCUS       DASTSF010000050         8337 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1080163,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000050 DASTSF010000000
VERSION     DASTSF010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 8337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8337
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1080163"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1173
                     /locus_tag="VFU15_01545"
     CDS             <1..1173
                     /locus_tag="VFU15_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716479.1"
                     /translation="GPMGVTGPTGFGVGPTGPTGAPGTNGTNGAAGPTGADGVTGPTG
                     VAGTNGTNGANGATGPTGAAGINGTNGATGATGVAGTNGINGATGPTGATGTNGTNGA
                     TGPTGAAGTNGTNGTNGAAGPAGPTGAAGTNGANGVTGPTGVAGTNGTNGSTGPTGAA
                     GTNGTNGATGPTGPDGFQNMMVFTSNGTFNVPAGVTQVYVKVWSGGGGGGAGCSVCLP
                     GSMPEGGTGGGGGGYCEALVTVTPSTAVPVTIGSGGAGGFGDGANGANGGNSSFGAFV
                     VVQGGAGGIHNSSGALGGWTSACTCVSGNQVSGQVSQSSSQVSAQVAMGGPGGDAGMA
                     GGGGAAGGALPFAFISGKQGTNGNVPGGGGGGGGEYIGGNSGAGGWGASGMIIVYY"
     gene            1347..1970
                     /locus_tag="VFU15_01550"
     CDS             1347..1970
                     /locus_tag="VFU15_01550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716480.1"
                     /translation="MAPFLKTYLPVHLLAGVSAAMTLSAQAVISPFNASGGTPLSSSS
                     VSEIISRFPSVFRKNEGQWPSDVLCRSVSAEGEKESHVNCFRGAAQVPDARDYSVVNY
                     NHGYNNIDLRYYACDEKTEYDFVAEPGGNAQDVVTGCSGIVPLFVAQDHSLHIVTAFG
                     GPEETTPVACQLINGDGMNDTFGGTGTGITDLQPATRQYMGHVTIVR"
     gene            1980..2681
                     /locus_tag="VFU15_01555"
     CDS             1980..2681
                     /locus_tag="VFU15_01555"
                     /inference="COORDINATES: protein motif:HMM:NF026253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HEU4716481.1"
                     /translation="MRTKTIFFFLFLFLTVKMSAQLTRYYEGGKTGYKNASGEIVVSP
                     QYDYGSEFSEGFALVMLGRERFFINEKGEDVFGKRFYDASCFHEGLAKVSTHGQYGFI
                     NTKGETVIEEQYAVAGDFSCARARVQVNGKFGFIDQSGKMIIPAQYDDASDFSDGLAA
                     VKQDGKWGFVSADGKISIGVQYDFATGFHNGEALVHKKDEKYYIDTKGKKTREIEEDD
                     DEEERNERGDHKKRF"
     gene            2683..6420
                     /locus_tag="VFU15_01560"
     CDS             2683..6420
                     /locus_tag="VFU15_01560"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HEU4716482.1"
                     /translation="MGSSRKFFAAVVLAFFACSVNAQNNVWTWMKGSSTANSTGSYGT
                     MGVASPANNPPARYEPAEWTDNQGRFWMYGGFEFVGNNTYADLWMYNPATNMWTWMNG
                     SNTSNIMPVYGTQGVPAPGNTPGSRKYAMATWTDLQGNLWLFGGFTPSGIGNDLWKYD
                     MTLNEWVWMKGQNTAFPAGSYGTIGVPNSTNEPSYRTETSATWVDNNGDLWFFGGQGN
                     GNFNDLWRYNIATNTWTWMNGSQFGFAAGNWGTMGVASPTNVPSGRMVYASWKDLAGN
                     FWLFGGNDSSFFSGGGSHNDLWKYDPVANMWTWMNGTNINNDPGSYGAQCVPSATNTP
                     PSRMECRTRWTDDCGNLWLFGGAINPGGTGGFNDLWKYNVQTNEWTWVSGANTWNQNA
                     VYGTQTVPNAANHPAWRMGSVGWKNNQGLWLFGGANSNTNFFNDVWKYTPDKPTAAFT
                     GTPTSGCAPLTVQFTDQSLPNCTDINTWSWNFGDPASGVNNVSSLQDPSHVFNAQGSY
                     TVTLVVTSCLGLSDSATLTINANTGLNLSATTSPATCGNPNGSATVSISNGTGPFTYA
                     WSSGGNAATENNLAAGNYTCTVTDANGCQGTQTVSIANNSGLNIAVASQNDVSCNGGT
                     NGTATINPSGGTGPYTFSWAPSGGNNATANNLPAGMYTVTVTDQASCSSTQTVAITEP
                     PVITLSLTAVPATCGNSNGSADVNAGGGAGGFLYSWQPSGGNASSATNLAAGNYTVTV
                     TDANGCQSTDSVQVNGSGTMSSSLSAQTDVLCNGGTNGSATVNASGGSGTYSYNWLPS
                     GGTSATANNLSAGTYTVTISDTGGCTNTVNVTITEPPVISLTTSGNVAICTGSNATLL
                     ASATGGAGNFSYTWNPGAIVSNSVTVAPNSTATYTVNAVDANGCSSSDSLTVTVNQPV
                     TMSVSGSTLICAGQTTSVSASASNSGPYVWQPGNLSGSSVSVSPAVTTTYTVSVWDVC
                     QNVFVTDSVTVAVENVMSQFVQTPSQGCGPLLVGFTDQSFVNSGTITGWSWNFGDGDT
                     SSVQNPVHTFSIPGTYTVTLSVTTSNGCSQSAAGSQVVDVWPSPLAGFSVYNIPVTTL
                     DPTADFVDQSVGAVTWQWDFGDGNTGTLQNPSHQYPADTGSYFITLVVSNSYGCVDTA
                     TGELNVLDDFTFYVPNAFTPNGDGLDELFTGYGTGIEKFHMMVFDRWGNLFYQTDDIS
                     QGWDGTYKGKKVQEDVYVYKIDITDIFLKDHHYIGHVSVVR"
     gene            complement(6470..7552)
                     /locus_tag="VFU15_01565"
     CDS             complement(6470..7552)
                     /locus_tag="VFU15_01565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716483.1"
                     /translation="MRSLTEEQIDFIREDIRRRGVFTESLQEDLLDHICCFIEEQPDD
                     AGPFGDVYRRALDAFGAQGLGGVQDELLSVFNHKYLKPMKKLMYISGAFASIALLAGA
                     FFKIQHWPGAGILLVSGTLVLTFFFLPFIFYLQFKEQTEKKGKVITAIGMLTAIMLCA
                     GALFKIMHWPGTIVMIVGFALLFVVFLPLYLINGIRNPLTKLTSMSNGFLVACIGGNM
                     MLLSFRQPSFSMTESNALISRKQESLHNVLQKQWESRAATLPVACGDAVKTFRDSCEA
                     ALFLFPQANPQDIVGDGALTDPELLPQFNQRIRADINALNAAMQQQNGWQKIEPSDYG
                     STVQGNLRFEILQLECDAYLRASALK"
     gene            complement(7545..7889)
                     /locus_tag="VFU15_01570"
     CDS             complement(7545..7889)
                     /locus_tag="VFU15_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007657775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEU4716484.1"
                     /translation="MYSSELIRGTLKTIVLKLLKEEGKMYGYEITQKVKDLTAGKIQI
                     TEGALYPTLHSLEAEGFVTTETVHIGRRVRKYYKLTTDGRATARKKLSEMADFLGTMR
                     ILFGSDAGMKHA"
     gene            8031..>8337
                     /locus_tag="VFU15_01575"
     CDS             8031..>8337
                     /locus_tag="VFU15_01575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716485.1"
                     /translation="MKISLAAVLFLLCFHMNAQGIFGGRSYGFTAVNYGFMFEMCAAA
                     PHATGFGMGILAEKFSIRGFGCRTLPAITLIQVPVKNAVVESTFLDLPASLLWRPGMR
                     "
BASE COUNT         1957 a         2141 c         2438 g         1801 t
ORIGIN      
        1 ggaccgatgg gtgttacggg tcctacaggt tttggtgtcg gccctacagg tccgaccggc
       61 gcaccgggaa cgaatggaac aaatggcgct gccggcccga ctggtgcgga tggcgtaacc
      121 ggaccgacgg gtgtcgcggg aacaaacgga acgaatggtg cgaacggagc aactggtccc
      181 acgggcgcag cgggaattaa cggaacaaat ggtgccaccg gcgctaccgg cgttgcagga
      241 accaatggaa taaatggcgc aacgggtccc actggcgcca caggaacaaa cggaacgaat
      301 ggtgcaacgg gtcctaccgg cgctgcaggt acgaacggta ccaatggtac gaatggcgct
      361 gcaggtcctg ccggccccac gggtgcagca ggaaccaatg gagcaaacgg cgtaacaggt
      421 cccacgggtg tcgccggaac caatggaacg aatggatcta ccggtccgac gggagctgcc
      481 gggactaacg gaacaaatgg tgcgacggga ccaaccggtc ccgatggttt ccagaatatg
      541 atggtgttca cttcgaacgg aacgttcaat gttcccgcag gtgtgacgca ggtatacgtg
      601 aaagtgtgga gcggaggcgg aggaggcggt gcaggttgtt ccgtgtgcct gccgggttcc
      661 atgccggaag gcggaacagg tggtggcggc ggcggctatt gcgaagcgct ggttacagtc
      721 acgccaagca ctgctgttcc ggttacgatc ggttcgggtg gtgccggcgg attcggtgac
      781 ggtgcaaacg gtgcgaacgg gggaaattct tcattcggtg cgtttgtcgt tgtgcagggt
      841 ggtgcaggcg gaattcataa ctccagtggt gcgctcggcg gatggacgtc ggcgtgcaca
      901 tgcgtgtcgg gcaaccaggt ttccggccag gtttcccaat cgtccagcca ggtgtcggca
      961 caggtggcta tgggcggacc cggcggtgat gcaggaatgg caggtggcgg aggcgctgcc
     1021 ggcggggcat tgccttttgc gttcatctcg ggcaaacagg gaaccaacgg aaacgtgccg
     1081 ggcggcggtg gtggtggtgg cggagaatac atcggcggta actccggggc aggaggatgg
     1141 ggcgcctccg ggatgatcat cgtttattat taaaaatcag catggaaaaa atcgcaatga
     1201 cgatcataac cgcgcttact gtttttactg cagggaacct gcccgctcac ggtatttatt
     1261 ttatcaatct aaccaacaac gaacagaaac acccggggaa atttatacga caatgaattt
     1321 ccccgcagga gagtttctga aaaaaggtgg ctccttttct gaagacatac ctgcccgtcc
     1381 atctccttgc aggagtttcc gccgcgatga cattgtcagc gcaggctgtg atttccccct
     1441 ttaatgcatc cggcggaact cctctttctt cttcctctgt ttcggaaata atctcgcgct
     1501 ttccttccgt gttcaggaag aacgaaggac agtggccttc cgatgttctc tgccgttccg
     1561 tcagcgctga aggagaaaag gaaagtcatg tgaattgttt ccgcggcgcc gcgcaggtgc
     1621 cggatgcgcg cgattattcc gtggtgaatt ataaccacgg ttacaataat atcgacctgc
     1681 gttattatgc ctgcgatgaa aagacggaat acgatttcgt cgcagagccc ggtggaaatg
     1741 cacaggatgt cgtgacggga tgctccggca ttgtgccgct ttttgttgcg caggatcatt
     1801 cgcttcatat tgttactgct ttcggtggac cggaagaaac gacgcctgtc gcttgccagc
     1861 tcattaacgg cgatggcatg aacgacacat tcgggggaac aggcacaggc attacggatc
     1921 ttcaaccggc aaccaggcaa tacatgggtc acgtcaccat tgtccgctaa atgaaaaaca
     1981 tgagaacaaa aaccattttc ttttttctct tcctcttttt aaccgtgaag atgagcgcac
     2041 agctcacccg gtattacgaa ggaggaaaaa ccgggtataa aaacgcctcc ggggaaattg
     2101 tcgtatcgcc gcaatatgat tacggcagcg aattcagcga aggcttcgca ctcgtgatgc
     2161 tcgggcgcga acgttttttc atcaatgaaa aaggagaaga tgttttcgga aaacgttttt
     2221 acgatgcctc ctgctttcat gaaggcctgg cgaaagtgag cacgcacggg cagtacgggt
     2281 ttatcaatac gaaaggagag acggtcattg aagaacaata tgcggtggcc ggggactttt
     2341 cctgtgcacg tgcgcgggtg caggtgaacg gaaagttcgg gttcatcgat caaagcggga
     2401 agatgatcat tcccgcgcaa tacgatgacg cttccgattt cagcgacggg cttgcggcgg
     2461 taaagcagga tgggaaatgg ggattcgtca gcgctgacgg aaagatttcc atcggcgtgc
     2521 aatatgattt tgcgacggga tttcacaacg gcgaagcgct cgtgcataag aaagatgaaa
     2581 aatattacat cgatacgaaa gggaagaaaa cgcgggagat agaagaagat gatgacgaag
     2641 aagaacgcaa tgaacgcggt gatcacaaaa aacggtttta agatgggaag ctcacggaaa
     2701 ttttttgcgg cggtcgtcct ggcgtttttt gcatgcagcg taaatgcgca gaacaatgtg
     2761 tggacatgga tgaaagggag cagcactgcg aacagcaccg gcagctacgg gacgatgggc
     2821 gtcgcttcac cggcgaacaa cccgcctgcg cgttacgagc ctgcggagtg gaccgacaac
     2881 cagggacgtt tctggatgta cggcggattc gaatttgtag ggaacaacac gtacgccgat
     2941 ctctggatgt acaatcccgc aacgaacatg tggacgtgga tgaacggatc gaatacgtcg
     3001 aacatcatgc ccgtgtacgg aacacaggga gttcccgcgc cgggcaacac gccgggctcg
     3061 cgcaaatacg cgatggccac gtggacggac ctgcagggaa atctgtggct gttcggcggc
     3121 ttcacgcctt ccgggatcgg gaatgacctg tggaaatacg acatgacgct gaatgaatgg
     3181 gtgtggatga aaggacaaaa caccgcgttc ccggcgggaa gctacggcac catcggcgtt
     3241 ccgaatagca cgaatgaacc gagctaccgg acggaaacgt ccgcgacatg ggtcgacaac
     3301 aatggcgacc tctggttttt cggcgggcag ggaaacggga atttcaatga tctctggcgt
     3361 tacaacatcg caacgaatac gtggacatgg atgaacggaa gccagttcgg ttttgccgcg
     3421 ggtaactggg gaacgatggg cgtagcgtcg ccaacgaatg ttccttcagg aagaatggtg
     3481 tacgcgtcgt ggaaagacct cgccgggaat ttctggctgt tcggtggaaa tgattcttct
     3541 tttttttccg gcggaggaag tcacaacgat ctctggaaat atgacccggt ggccaacatg
     3601 tggacgtgga tgaacgggac gaacatcaac aacgatcccg gcagctacgg tgcgcaatgc
     3661 gttccttccg caacgaatac gccgccgtcg agaatggaat gcagaacgcg gtggaccgac
     3721 gactgcggaa acctctggct gttcggaggc gcgatcaatc ccgggggaac cggtggtttc
     3781 aacgacctgt ggaaatacaa cgtgcagacg aatgaatgga cctgggtgag cggcgcgaac
     3841 acatggaacc agaatgcagt ttacggaacg cagaccgttc ccaatgcggc gaatcaccct
     3901 gcgtggagaa tgggttccgt cgggtggaag aacaaccagg ggttgtggct gttcggcggc
     3961 gcaaattcga atacgaattt tttcaatgac gtgtggaagt atactcccga caaaccgacc
     4021 gcagccttca ccggaacgcc tacatccggc tgtgcgccgc tgaccgtgca gttcaccgac
     4081 cagtcgctgc cgaattgcac ggacatcaat acatggtcgt ggaatttcgg cgatcccgct
     4141 tccggcgtca acaatgtttc ttcactgcag gacccttcgc acgtattcaa cgcgcaggga
     4201 agctacacgg tgacgctcgt cgttacgtcc tgtctggggc tttctgattc ggcgacattg
     4261 acgatcaacg cgaacaccgg gttgaatttg tcggcgacaa cttctcccgc cacgtgcgga
     4321 aatccgaacg gatcggcgac ggtatccatt tccaacggga caggaccttt cacttatgct
     4381 tggtcgagcg gcggaaacgc agcgacggaa aataatcttg ctgccgggaa ttatacgtgc
     4441 acggtgacgg atgcgaacgg ttgccaggga acacaaacgg tttccattgc gaacaacagc
     4501 gggctcaata ttgctgtcgc atcgcagaac gatgtgagct gcaatggcgg aacgaatgga
     4561 acagcgacga tcaatccgtc aggcggaaca ggtccgtata cattttcatg ggcgccgtcc
     4621 gggggaaaca acgcgaccgc aaataatctc ccggcgggaa tgtatacggt gacagtaacg
     4681 gaccaggctt cctgttcgtc cacgcaaaca gttgcgatca cggaacctcc cgtcattaca
     4741 ctttcgctta ccgccgttcc tgcaacttgc ggcaacagca atggaagtgc ggacgtgaat
     4801 gcaggcggcg gtgcaggagg atttttgtat tcctggcaac cttcgggggg aaatgcttct
     4861 tccgcgacga atcttgccgc agggaattat acagtaaccg tcaccgatgc aaacggctgc
     4921 cagtcgaccg acagtgtgca ggtgaacgga agtggaacga tgagcagctc tttgtcggcg
     4981 cagaccgacg tgttgtgcaa cggtggaacg aacggcagtg caaccgtaaa tgccagcggc
     5041 ggcagcggaa cgtatagcta caattggctg ccttccggcg gaacgagcgc aacggcgaac
     5101 aatctttccg caggaacgta caccgttacg atttccgaca ccggcggctg cacgaatacg
     5161 gtgaatgtaa cgatcacaga gccgccggta atttcactga ccaccagcgg gaatgttgcg
     5221 atctgcacgg gcagcaatgc gacactgctc gccagcgcaa caggcggcgc cggaaatttt
     5281 tcctacacat ggaatcccgg ggcgatcgtt tcgaattctg ttaccgtcgc gccgaattca
     5341 acggcaacct atacggtgaa tgcggtcgat gcgaacggat gttcttcttc cgattcactg
     5401 acggtaacag tcaaccagcc ggtgacgatg tctgtttccg gcagcacgct gatctgtgca
     5461 ggacaaacaa cttccgtcag cgcgagcgca tcgaacagcg gaccttacgt gtggcagccg
     5521 ggaaatcttt ctggcagcag cgtgagcgtt tcacctgcag tgacaaccac gtacactgtt
     5581 tccgtgtggg atgtgtgcca gaatgttttc gttacggatt cggtgacagt tgcggttgaa
     5641 aacgtgatgt cgcagttcgt gcagacgccg agccagggct gcggtccgct gctggttggg
     5701 ttcacggacc agtcgttcgt caacagcgga accatcaccg gctggagctg gaatttcggc
     5761 gacggcgata cttcatccgt gcaaaatccg gtgcatacat tcagcattcc cgggacgtac
     5821 acggtcacgc tttccgtcac cacgtcgaac ggatgttcgc agagtgcggc cggttcgcag
     5881 gtggtggatg tatggccttc accgctggcc ggtttttccg tctacaacat tcccgtgacg
     5941 acgctcgatc ctacggctga tttcgtcgac cagtcggtgg gcgcggtaac gtggcaatgg
     6001 gatttcggcg acggcaacac gggtacgttg cagaatcctt cgcaccagta cccggcggat
     6061 acgggaagct atttcatcac gctcgtcgtt tcgaacagct acggctgcgt ggacacggcc
     6121 accggcgaac tgaacgtgct ggatgatttc acgttctatg ttccgaacgc attcacaccg
     6181 aacggcgacg ggctcgatga attattcacc ggttatggaa caggcataga aaaattccac
     6241 atgatggtgt tcgatcgctg gggaaatctt ttttaccaga ccgatgacat ttcacagggc
     6301 tgggacggaa catataaagg gaaaaaagtg caggaagatg tgtacgtcta caagatcgac
     6361 atcaccgata tctttttaaa agaccatcac tacatcggtc atgtgagcgt ggtgcgttga
     6421 tgaaaagaaa aaacggcggg aaaaatctcc tgccgttttc cgttacttat tacttcaatg
     6481 ctgaggcgcg caaatacgca tcacattcca gctgcaggat ctcgaagcgc aggttgccct
     6541 gcacggtcga tccgtaatcg gaaggttcga tcttctgcca gccgttctgc tgctgcatgg
     6601 cagcgttcag cgcattgata tccgcccgga tccgctggtt aaattgcgga agaagttcgg
     6661 ggtctgtcag cgccccgtcg cccacgatgt cctgcggatt cgcctgcggg aaaagaaaga
     6721 gtgcagcttc gcaactgtcg cggaatgttt tgacggcatc accgcacgcg acgggaagtg
     6781 ttgctgcccg cgattcccat tgcttctgca gaacgttgtg cagcgattcc tgtttccggc
     6841 tgatgagcgc attcgattcc gtcatcgaga agctcggctg gcggaaggaa agcagcatca
     6901 tgttcccgcc gatgcacgcc acgagaaaac cgttgctcat cgacgtcagc ttcgtgagcg
     6961 ggttgcggat accgttgatc aggtagagcg gaaggaaaac aacgaacagc agcgcaaacc
     7021 cgacgatcat cacgatcgtt ccgggccagt gcatgatctt gaacaaggcg cccgcacaaa
     7081 gcatgatcgc ggtgagcata ccgatggcgg tgatcacttt tcctttcttc tccgtctgtt
     7141 ctttgaactg cagataaaaa atgaacggaa ggaaaaagaa cgtgagcacg agcgtaccac
     7201 tcacgagcag gattcccgct cccggccagt gctgaatttt gaagaacgcg ccggcgagca
     7261 gtgcaatact ggcgaaggcg cccgagatat acatgagttt tttcattggc ttgagatatt
     7321 tgtggttaaa aacagacagc agctcatcct gcacgccgcc gagtccctgc gcgccaaaag
     7381 cgtccagggc ccgccggtag acgtccccga acggccctgc atcatcgggc tgctcttcga
     7441 taaagcagca gatgtggtcg aggaggtctt cctgcagcga ttccgtgaag acgccgcgcc
     7501 tgcggatatc ttcacggatg aaatcgatct gttcttccgt caggctacgc atgcttcatg
     7561 cccgcgtcac tgccgaaaag gatgcgcatc gtccccagga aatcggccat ttcactgagc
     7621 tttttgcgcg cggtcgcacg tccgtcggtg gtcagcttgt aatacttccg cacgcgccgg
     7681 ccgatgtgca cggtttccgt agttacaaaa ccttccgctt cgagactgtg caatgtggga
     7741 tagagagcgc cttcggtgat ctggatcttg ccggccgtaa gatctttcac cttctgcgtg
     7801 atctcgtatc catacatttt tccttcctct ttcagcagct tgagaacaat cgttttcaat
     7861 gttccccgga tgagttcgct tgaatacata cgtaaatata cataagaata ttatgtataa
     7921 gcaaacaaat gcataaaaat cttatgtatc tggcgccgcc cgggcttccc ggcaacgatt
     7981 tcctttttct tcgtctttct gccggaacag ctttacgatt ttctttcctt atgaaaattt
     8041 cactcgcagc agtgctgttc ctgctttgct ttcacatgaa cgcacaaggt attttcgggg
     8101 gccgctcgta tggtttcacg gccgtgaatt acgggttcat gttcgaaatg tgcgctgctg
     8161 ctccgcatgc aacaggtttc gggatgggga tccttgccga aaaattttca atacggggtt
     8221 tcggctgccg taccctgcct gccatcacgc tcatccaggt gcctgtaaaa aatgcagttg
     8281 tggaatccac gttcctcgat cttccggctt ccctcctgtg gcgtcccggt atgcgtg
//
LOCUS       DASTSF010000051         9130 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_187040,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000051 DASTSF010000000
VERSION     DASTSF010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 9130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9130
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_187040"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..537)
                     /locus_tag="VFU15_01580"
     CDS             complement(<1..537)
                     /locus_tag="VFU15_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018478009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase domain-containing
                     protein"
                     /protein_id="HEU4716486.1"
                     /translation="MKTTTPAKVLLAFSGGLDTSFCATWLSAEKGFDVHAVTIDTGGF
                     TENELAAIEKRAHDLGVKSFAVIDGTKRFYESCLRFLVFGNILKNGTYPLSVSAERVV
                     QAMLLAEHAQLTGITHIAHGSTGAGNDQVRFDMLLDILLPGVEIITPIRDLKLSRQQE
                     IDYLVQHGFHFDFTKAAYS"
     gene            complement(545..1231)
                     /locus_tag="VFU15_01585"
     CDS             complement(545..1231)
                     /locus_tag="VFU15_01585"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009184983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU4716487.1"
                     /translation="MEDHTEVKVLVAGAQHTSYAQAICDEMEASAKARGTGIARRSPD
                     YVAQKMLEGKAVIALTVSGKWAGFCYIETWGHGQYVANSGLIVSPDFRRSGLARRIKE
                     EIFRLSRKKYPDAKIFGLTTGLAVMKINSDLGYEPVTYSELTDDEAFWKGCQSCVNFE
                     ILKSKERKNCLCTAMLFDPADQKKSSRETPGEKFRRRSKLYDRWMRIKQHRLLKGIRK
                     NKNSITSLFF"
     gene            complement(1361..1489)
                     /locus_tag="VFU15_01590"
     CDS             complement(1361..1489)
                     /locus_tag="VFU15_01590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716488.1"
                     /translation="MKHLFYMAISMIKQAVRVNRRRYVYVTNVVCSSPRRTFRSRI"
     gene            complement(1626..3974)
                     /locus_tag="VFU15_01595"
     CDS             complement(1626..3974)
                     /locus_tag="VFU15_01595"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEU4716489.1"
                     /translation="MLRSIAVLLSALFFATSVNAQIVPDRAPVQVGPVDQPQEKVGVV
                     LSGGGASGLAHIGVLKALEENNIPIDYICGTSIGALVGCLYCMGYSPAEIETLVKSDE
                     FKSWATGTFQPNQIYYFKKKDDNASWITFKLSLDTALSTNLPTNIISPVQMDFALMEK
                     TAGASAACGNNFDSLMIPFRCVASDVANKESVIFRNGDLGQAVRASMSYPFYLKPIRV
                     NGKLLFDGGLYNNFPSNVMYDDFFPDFIIGSNVASPFLEPDEDNLYSQVRAMLTSRTD
                     FDPKCENGIVVEPNADWLGLFDFDNPQQIIDSGYYATMRKMDSIKVAVQRRSDPSVLA
                     AKRAMFRAKMPRIVFDNIDIEGPGLKKRQEAYVRRMLRHRNRFVDIETLKPGYFRLAA
                     DDQIQSMYPIASYNKTTGYFDLRLTIKKEKKVFTELGGNFSNRPITTGFVGLQYNIFS
                     NPSISLMGNGYFGKLYQSAQVRARIDFPTRIPFYLEPILTWNRYDYYRSTPVYFVDTR
                     PPYLNRIDQFGELDLGFPIRNKGKFVLGTGDAFVRYLYYQTSQFTSADTTDRTDLSIL
                     TTHFAYERNTLNRKQFATSGTYFSIRGRFVQAEEFYDPGSTAIGDAEYRKIHNWLQFR
                     LTYDTYFKERGKWRLGFYGEAVYSNQPFLHNYTSSILSAPAFLPTAESPTFFIPQFHA
                     FQYAGAGLKSVFVLWKNIDLRFEGYVFMPYQDIVSGPGNVAEYAAPLSISSMRYMGMA
                     AAVWNTPLGPISLSLNYYSPKQYPYSILFNFGYLIFNRSALD"
     gene            complement(4080..4883)
                     /locus_tag="VFU15_01600"
     CDS             complement(4080..4883)
                     /locus_tag="VFU15_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007655382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HEU4716490.1"
                     /translation="MSFPQTFTDQMGREIHLPARPQRIVSLVPSQTELLHDLGLEHEV
                     AGITKFCIHPESWFRSKKRVGGTKQLNFEAIRALQPDLIIGNKEENEEEQVLALMNEF
                     PVWMSDIRTLDDALAMIKALGNITGTGEKAGAIAGDIREKFSAFDREAASFPEKTVAY
                     FIWKNPFMVAGNGTFIHSMLGRCGWKNVFSDLPGRYPEITIQDLQEKNPELVVLSSEP
                     YPFAEKHVGEFSLHLPQSKVIVADGEMFSWYGSRLRLAPGYFSALIKSL"
     gene            complement(4951..6330)
                     /locus_tag="VFU15_01605"
     CDS             complement(4951..6330)
                     /locus_tag="VFU15_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014679485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEU4716491.1"
                     /translation="MTPEKQVFDNILGTIGHTPLVRINTITKGLTKAKIYAKVETFNP
                     GNSIKDRMALKMIEDAEKAGLLKPGGTIIEGTSGNTGMGLAIAAIIKGYKCIFTTTDK
                     QSKEKVDALRAFGAEVIVCPTNVDPEDPRSYYSVSSRLEKEVKNSWKPNQYDNLSNSQ
                     AHYEQTGPEIWEQTEGKITHLVVGVGTGGTISGTAKFLKEKNPAVKVWGIDTYGSVFK
                     KYKETGIFDKNEIYPYITEGIGEDFLPQNVRFDLIDLFEKVTDKDAAVMTRRIAREEG
                     IFAGNSAGSAMAGLVQLAPKLKDDDVVVVIFHDHGTRYLGKMFNDDWMREKGFLDAKG
                     QTAKDIVASLKHAKLASVETSEPISAAVKMMRENDFSQMPVTQNGRIVGAINEQQLFD
                     CLVHNPENKTAPVETIMQPAFPFADISTPVEDLSTMITPENPAVLVRDFKTDDTFIIT
                     RHDIIRALC"
     gene            complement(6469..6867)
                     /locus_tag="VFU15_01610"
     CDS             complement(6469..6867)
                     /locus_tag="VFU15_01610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716492.1"
                     /translation="MLKKVLPFVFTLLLIASCRYDHVPDIAAVPPGCDTLHPSYHNCV
                     VPILKANCYSCHSDSASNHGQSTFDMEDFTLFKNELNLSYQGDTTYGSKFMAIIGQRP
                     LIIHMPPTGKLPQQDINTIQRWVTARAPEN"
     gene            complement(6918..8204)
                     /locus_tag="VFU15_01615"
     CDS             complement(6918..8204)
                     /locus_tag="VFU15_01615"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU4716493.1"
                     /translation="MLIRLIAFLGSGFSFFERFVSRRMLVQTDSSGRMSRPISRVAVG
                     GIAVGLAIMILTLAVMNGFQQEIRRKVIGFGSHIQITGFDNNNSFETDSVNRHQPTVD
                     ALRSMPGIRHIQVYATKAGIIKSGAQLMGVLLKGIDTDFDWTFFKKNLKEGNLFVLPP
                     RTPQHPDTATEDVLVSKYIADKMELKVGKKFKMFFLRDNRTRPRIFRVAGIYETGLTD
                     GFDDRFVLCDIRQIQQLNDWDSSLVSGFEVLVKDFSDLDTATAEVNNTIDINLYAQSI
                     RELNPQIFSWLDVLDVNALIIITLMIFVSMINMISALIILILDRTNMIGILKAMGARN
                     MSIRKIFFLHAVRLIAFGMLIGNILGLALCWVQWKFQLVSLDQESYYLKTVPIEINWT
                     YFALLNGAVMICCWAALILPSMIVSRITPVKAIRFS"
     gene            8340..>9130
                     /locus_tag="VFU15_01620"
     CDS             8340..>9130
                     /locus_tag="VFU15_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017842958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HEU4716494.1"
                     /translation="MKRIIPFLLALLFFSSARAQIVIRAQTNEEKTDADIQTGAERTD
                     VYLPWLKGKKVAVVANQTSMINSTSLVDSLISLKVAVKKIFCPEHGFRGDADNGEDVG
                     NTTDKKTGLPVISLYGDHLRPTVKDLKGIDVVVYDIQDVGCRFYTFITTMTYVMQACA
                     DQHKTFIILDRPDPNGYYVDGPVLEPEFKSFVGLHPVPIVYGMTVAEYAKMVNGEKWL
                     DNGAQCDLKYVECVGYSHTDWYQLPVPTSPNLPNMASVYLYPSLCL"
BASE COUNT         2008 a         2341 c         2569 g         2212 t
ORIGIN      
        1 cgagtaggcc gctttggtga aatcgaaatg aaatccgtgc tgaacaaggt aatcgatctc
       61 ctgctgccgg gaaagtttca ggtcgcggat gggcgtaatg atctccacgc ccggcaggag
      121 aatatcgagc agcatatcga agcggacctg gtcgttgccg gcgcccgtgc tcccgtgtgc
      181 aatgtgcgtg atgcccgtga gctgcgcatg ttccgcgagc agcatggcct gcaccacgcg
      241 ttcggcgctg accgaaagcg ggtacgttcc gttcttcagg atgttcccga aaacgaggaa
      301 gcgcaggcac gattcataaa agcgtttcgt tccgtcgatg acggcgaacg atttcacgcc
      361 gagatcatgt gcgcgttttt cgatggccgc cagctcgttt tccgtgaacc cgccggtatc
      421 gatggtaacg gcgtgcacgt cgaaaccttt ttcagcggag agccacgttg cacagaagct
      481 ggtgtcgagt ccgccgctga acgcgagcag gactttcgcg ggagtggttg ttttcatatt
      541 catgtcagaa aaacaggctg gtgatcgaat ttttattttt tcgtattcct ttcagcaggc
      601 gatgttgctt gatgcgcatc caccggtcgt agagcttcga ccggcgcctg aatttttcgc
      661 cgggtgtttc cctggaagat tttttctggt cggcgggatc gaacagcatc gcggtacaga
      721 gacagttttt gcgttccttg cttttcagga tctcgaaatt gacacagctc tggcatcctt
      781 tccagaacgc ctcatcgtcg gtgagctcgg aataggtgac gggttcgtaa ccgagatcgc
      841 tgttgatctt catcacggcg agaccggtgg tgagcccgaa aatttttgca tcgggatatt
      901 ttttccgcga cagccggaaa atttcttcct tgatccgccg cgccagcccg ctccgccgga
      961 aatccggcga gacgatgagt cccgagttcg ccacatactg cccgtggccc caggtctcga
     1021 tgtagcagaa gcctgcccat ttaccggaaa cagtaagcgc gattactgct tttccttcga
     1081 gcattttctg cgctacgtaa tccggcgaac gccgggcgat accggtgccg cgcgctttgg
     1141 cggacgcttc catttcatcg cagatcgcct gcgcgtaaga cgtgtgctgc gcacctgcga
     1201 caagcacttt tacttccgtg tgatcttcca tgaggaaaac acgattggtc acggcgatgc
     1261 gtgacggtta aacatgcgct ttacccgttg cgggcagaag cagtgaataa atggtaaggg
     1321 agtagcggac cgtagccgct gccaggaatt tgcggatcga tcagatcctg ctgcggaagg
     1381 tcctcctcgg agaggaacat acaacgttcg tcacataaac atagcggcgg cggttcacgc
     1441 gaacggcctg cttgatcatc gagatcgcca tgtaaaagag gtgtttcatt tcacctgtcg
     1501 gatcagattg ttagacgtgt tgttttttca acggcgcaaa tatcttcata aaaaaacgag
     1561 aatgtcaaca ggttgcccga aaaattttgc gtgagagcaa aatttaacag gcggttgtcc
     1621 cggcttcagt cgagcgcact gcggttgaag atgagatagc cgaagttgaa aagaatgctg
     1681 tacgggtatt gtttcgggct gtaataattc aggcttaagc tgatcggccc gagcggcgtg
     1741 ttccagacgg cggcagccat tcccatgtag cgcatggacg agatcgagag gggcgccgcg
     1801 tattccgcga cattgcccgg accggaaaca atatcctggt aaggcatgaa cacgtatcct
     1861 tcgaagcgca ggtcgatatt tttccagaga acaaaaacgg atttcagccc ggcgccggca
     1921 tactggaagg cgtggaactg cggaatgaaa aatgtcggac tttccgctgt gggaaggaaa
     1981 gccggtgcgc tgagaatgga agaggtgtaa ttatgcagga agggctggtt cgagtacacc
     2041 gcttcaccgt aaaacccaag ccgccatttt cctctttcct tgaaatacgt atcgtacgta
     2101 agacggaact ggagccagtt gtggatcttc ctgtattcag catccccgat cgccgttgaa
     2161 ccgggatcat agaactcttc cgcctggaca aatcttccgc gaatggaaaa ataagttccc
     2221 gacgttgcga actgtttccg gttgagcgtg ttcctttcgt aggcaaaatg cgtcgtgaga
     2281 atgctaaggt cggtacggtc ggtcgtgtcg gcagaagtaa actgcgacgt ctggtaatac
     2341 aggtaacgca cgaaggcatc acctgtaccg agcacgaatt ttcctttgtt gcggatgggg
     2401 aagcccagat cgagctcacc gaactggtcg atgcggttga ggtaaggcgg ccgcgtgtcg
     2461 acaaaataaa ccggtgtcga tcggtaatag tcgtaacggt tccacgtcag tatcggttcg
     2521 agataaaacg gaatgcgtgt gggaaaatca atccgtgcgc gtacctgcgc ggattggtac
     2581 agctttccga aatagccgtt gcccatgagg ctgatggaag gattgctgaa aatattatac
     2641 tgcaggccga cgaagcccgt ggtaatagga cggtttgaaa agtttccgcc caattcggtg
     2701 aagacctttt tttctttttt gatcgtgagc cgcaggtcga aatagccagt cgttttgttg
     2761 tagcttgcga tgggatacat cgattgtatc tggtcgtcgg cggccagcct gaaataaccg
     2821 ggcttcagcg tttcaatgtc gacgaaacgg ttgcggtggc gcagcattcg ccgcacgtaa
     2881 gcttcctgcc gttttttcag ccccggtcct tcgatatcga tgttgtcgaa aacgatgcgc
     2941 ggcatttttg cacggaacat cgcgcgtttt gcggcgagca cggaaggatc gctgcggcgc
     3001 tgcacggcaa ctttgatgga atccatcttg cgcatcgttg cataataacc gctgtcgata
     3061 atctgctggg gattgtcgaa atcgaaaaga cccagccagt ctgcattcgg ctccaccacg
     3121 atgccgttct cgcatttcgg atcgaaatcc gtgcggctcg tgagcatggc gcgtacctgc
     3181 gaataaagat tgtcttcgtc cggctcgagg aaaggcgatg ccacgttgct gccgatgatg
     3241 aaatcaggaa agaaatcgtc gtacatcacg ttcgacggaa agttgttgta caggccgccg
     3301 tcgaaaagca gtttcccgtt cacgcggatc ggtttcaggt agaaagggta cgacatggaa
     3361 gcgcgcaccg cctggccgag atcgccgttg cggaaaatga cggattcctt gttggccaca
     3421 tcggaagcga cgcaccggaa cgggatcatc aggctgtcga aattgttccc gcacgcagcc
     3481 gaagcgcccg ctgttttttc catcagcgca aaatccatct gcaccggtga aatgatgttc
     3541 gtcggaagat tggtggagag cgccgtgtcg agcgaaagct tgaacgtgat ccacgaagca
     3601 ttgtcgtcct tcttcttgaa ataatagatc tggttcggct ggaacgttcc tgttgcccag
     3661 cttttgaact catcgctttt caccagcgtt tcgatctctg ccggcgaata gcccatgcag
     3721 tagaggcagc ccaccagcgc accgatggaa gtcccgcaga tgtaatcgat gggaatgttg
     3781 ttttcctcga gcgctttcag cacgccgatg tgcgccagtc ccgatgcgcc gccgccgctg
     3841 agcaccacgc ccaccttttc ctgcggctga tcgaccgggc ccacctgcac gggtgcgcgg
     3901 tcgggaacga tctgtgcgtt cacggaagtc gcaaaaaaaa gggcggagag aagtacggca
     3961 atgctccgga gcatagggaa atttcgagag ataaaggtac taaattcacc tccaacgggg
     4021 agggagttgg aaaggttgaa aggttgggag gttgaaaggt tggaaagtcg aaaaggagat
     4081 cagagggatt tgatcagcgc tgaaaaatat ccgggcgcaa gccgcagccg ggagccgtac
     4141 cacgagaaca tttccccgtc ggcgacgatc actttgcttt gcggcaggtg aagcgagaat
     4201 tccccgacat gcttttccgc gaaaggatac ggttcggaag acaggacgac aagctccgga
     4261 tttttttcct ggaggtcctg tatcgttatt tcgggataac ggccgggaag atccgagaag
     4321 acatttttcc atccgcagcg cccgagcatg gaatgaatga acgtgccgtt tcctgccacc
     4381 atgaacgggt tcttccagat aaaataggcg actgttttct ccgggaaaga cgcggcttcg
     4441 cgatcaaacg ctgaaaattt ttcacggatg tcgcctgcga tcgcgcctgc tttttcaccg
     4501 gttcctgtaa tgttcccgag cgctttgatc atcgccagcg catcatcgag cgtccggatg
     4561 tcgctcatcc acacggggaa ttcattcatc agcgcaagca cctgttcttc ctcgttctct
     4621 tccttgttgc cgataatgag atcgggctgc agcgcacgga tggcctcgaa gttcagctgc
     4681 ttggttccgc ccacgcgttt tttcgaacgg aaccagcttt cgggatgaat gcagaatttc
     4741 gtgatgccgg ccacttcatg ttcgagaccg agatcatgca gcagctctgt ctgcgaagga
     4801 acaagggaaa cgatacgctg cggtcgggcg ggaagatgga tttctcttcc catctggtcg
     4861 gtgaatgtct gcgggaagct catggttttc ttttgggtga attccgggag gcattctgct
     4921 ttcccgcagg ggattttgcc gtgcgcccgg tcagcagagc gccctgatga tgtcgtggcg
     4981 cgtgatgatg aacgtgtcgt cggttttgaa atcgcgcacg agcacggcag gattttcagg
     5041 cgtgatcatc gttgaaagat cttccaccgg cgttgaaata tccgcgaacg ggaacgccgg
     5101 ctgcatgatg gtttcaacgg gcgctgtttt gttctccggg ttgtgcacga ggcagtcgaa
     5161 cagttgctgc tcgttgatgg cgcccacgat ccttccgttc tgcgtgacag gcatctgtga
     5221 aaaatcattt tcgcgcatca tcttcactgc cgccgaaatg ggttcgctcg tttcaaccga
     5281 agccagcttt gcgtgtttca gcgaggcgac aatgtctttc gctgtttgtc cttttgcgtc
     5341 gaggaaacct ttttcgcgca tccagtcgtc gttgaacatt ttgcccaggt aacgcgtacc
     5401 gtgatcgtgg aatatcacaa caacgacatc gtcgtccttc agcttcggcg ccagctgcac
     5461 gagtcccgcc atcgccgatc cggctgagtt gccggcgaaa attccttctt cccgcgcaat
     5521 gcggcgcgtc atgacggcgg cgtccttgtc cgtcactttt tcaaacaggt cgataaggtc
     5581 gaaacggaca ttctgcggga gaaaatcctc gcctatgcct tcagttatgt acgggtaaat
     5641 ttcattcttg tcaaagatgc ccgtttcctt gtatttcttg aaaacggaac cgtacgtgtc
     5701 gatgccccac actttcacgg cgggattttt ttctttcagg aatttcgcgg tgccgctgat
     5761 cgttccgcct gttcccacac cgacgacgag gtgcgtgatc tttccttctg tttgttccca
     5821 gatctccggc ccggtctgct cataatgcgc ctgcgaattg gaaagattat cgtactggtt
     5881 cggcttccag gaattcttca cctctttttc cagccgcgac gaaacggaat aataggaacg
     5941 gggatcttcc ggatcgacgt tggtcgggca gacgatcact tcagcgccga aggcgcggag
     6001 cgcatccact ttttctttcg actgtttgtc ggtcgtggtg aaaatgcatt tgtatccttt
     6061 gatgatcgct gcaatggcga ggcccattcc cgtgttaccc gaagtccctt cgatgatggt
     6121 tccgccgggt ttcaatagtc ctgctttttc cgcgtcttcg atcattttca gcgccatgcg
     6181 gtctttgatc gaattgccgg gattgaaagt ttccaccttg gcgtaaattt tcgctttggt
     6241 gagccctttg gtgatggtgt tgatgcggac aagcggcgtg tggccgatcg tgccgagaat
     6301 attgtcgaat acttgctttt caggagtcac tggaaacgga ttttgagtat gcaaagataa
     6361 gtttccacac gggttatccg ggaagtgtcc tgaaatgata aatcatgaat gggcggatct
     6421 tcctgcgtgt cgctccggtt tcacagctga tatccggata cggcgtgttc agttctccgg
     6481 cgcacgcgcg gtcacccagc gctggatggt gttgatatcc tgctgcggca gttttccggt
     6541 cggcggcatg tggatgatca gcggacgctg gccgatgatg gccatgaatt tcgagccgta
     6601 ggtcgtgtcg ccctggtaag aaagattcag ctcgtttttg aacagcgtga aatcttccat
     6661 gtcgaacgta gactgcccgt ggtttgacgc cgaatcggaa tggcaggaat agcagttcgc
     6721 tttgagaatg gggacaacgc agttgtggta tgaaggatga agcgtgtcgc agccgggagg
     6781 aaccgcagct atatccggca catgatcata acggcacgaa gcgatcagca ggagcgtgaa
     6841 tacgaacgga agcacctttt tcagcacggt gcaaaagtaa atgcgtcatg taaactctgt
     6901 attaagtccg gtgtttttca gctgaaacgg atcgctttta ccggcgtgat gcgggaaacg
     6961 atcatcgagg gaaggatcag tgcggcccag cagcagatca tgacggcgcc gttcagcagc
     7021 gcgaagtacg tccagttgat ctcgatggga acggttttca ggtaatagga ttcctggtcg
     7081 agcgacacga gctggaattt ccactgcacc cagcagagcg ccaggccgag gatattcccg
     7141 atcagcatcc cgaacgcgat cagtcgcacc gcatgcagga aaaatatctt ccgtatgctc
     7201 atgttgcgcg cgcccatcgc tttcagtatc ccgatcatgt tcgtgcggtc gaggataaga
     7261 ataataagcg cgcttatcat attgatcatg ctcacgaaga tcatgagcgt aataattata
     7321 agcgcgttta catcgagcac gtcgagccag gaaaatatct gcgggttcag ttcgcggatg
     7381 ctctgggcat acaggttgat gtcgatcgtg ttgttcactt ccgccgtcgc ggtatcgaga
     7441 tcgctgaaat cctttaccag cacttcgaag ccggatacca ggctgctgtc ccagtcgttg
     7501 agctgctgga tctggcggat gtcgcacagc acgaaacggt cgtcgaaacc gtcggtgagc
     7561 cccgtttcgt aaattcccgc cacgcggaaa atacgcgggc gcgtgcggtt gtcgcgaagg
     7621 aagaacattt tgaatttctt ccccactttc agctccatct tgtcggcaat gtatttcgaa
     7681 accagcacat cttccgttgc cgtatcagga tgctgcggcg tacgcggcgg aagaacgaac
     7741 aggttccctt ctttcaggtt ttttttgaag aaggtccagt cgaaatccgt atcgatgcct
     7801 ttcagcagaa cgcccataag ctgggcgccc gatttgatga tgcccgcttt ggtggcgtac
     7861 acctggatgt ggcgtatgcc cggcatggag cgcagcgcat cgactgtcgg ctggtgacgg
     7921 ttgacggaat cggtttcgaa agaattgttg ttgtcgaatc ccgtgatctg gatgtgcgag
     7981 ccgaagccga tcaccttgcg ccgtatttcc tgctggaacc cgttcatcac ggccagcgtc
     8041 aggatcatga tcgcaagccc caccgcgatt ccgcccacgg ccacccgcga gatgggacgc
     8101 gacatgcggc cggatgaatc cgtctgcaca agcatcctcc gcgaaacaaa ccgctcgaag
     8161 aatgaaaaac cggaacccag gaatgcgata agccggatca gcatcggcca aaggtaagca
     8221 cttctcagca tggtgttttt cagtgcggac cgcggcgggt gtgcatcggc ggcctggtat
     8281 ttgccggtgg ttttcaaccg atgcgtagcg cgttttttcc tactttcact tgccgtatta
     8341 tgaaacgaat cattcccttc ctgcttgcgc tcctcttctt ttcatccgcc cgcgcgcaga
     8401 tcgtgatccg cgcacagacc aacgaagaaa aaaccgatgc ggatatacag acgggtgccg
     8461 agcgcacgga tgtttatctt ccctggctga aaggaaaaaa ggtggcggtg gtggccaacc
     8521 agacgtcgat gatcaattca acatcgctgg tggattcgct catttcgctt aaagtggcgg
     8581 tgaaaaagat tttctgcccc gaacatggtt tccgcggcga cgcggataac ggggaagacg
     8641 tgggaaatac caccgacaaa aaaaccgggc ttcccgttat ttcattgtac ggcgatcatc
     8701 tccgcccgac ggtaaaagac ctgaaaggca ttgacgtggt ggtgtacgat atccaggatg
     8761 tgggctgccg tttttataca ttcatcacca cgatgacata cgtgatgcag gcctgcgccg
     8821 atcagcataa aacgttcatc atcctcgacc gcccggatcc caatggttat tacgtggacg
     8881 ggcccgtgct cgagccggaa ttcaaatcgt tcgtgggact tcatcctgtt cccattgtgt
     8941 acgggatgac cgttgcggag tatgcgaaaa tggtgaacgg ggaaaaatgg ctcgacaatg
     9001 gcgcacagtg cgacctgaaa tacgtggaat gcgtcggcta ttcgcacacc gactggtacc
     9061 agctgcccgt cccgacatcg ccgaatttgc cgaacatggc ttccgtttat ctctatcctt
     9121 cgctttgtct
//
LOCUS       DASTSF010000052         7473 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1974559,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000052 DASTSF010000000
VERSION     DASTSF010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 7473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7473
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1974559"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..435
                     /locus_tag="VFU15_01625"
     CDS             <1..435
                     /locus_tag="VFU15_01625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c peroxidase"
                     /protein_id="HEU4716495.1"
                     /translation="EASLTSQEADGLATFRTKCAGCHPEPLFTDEGFHNIGLPVDNTL
                     NDEGRMHITLDPADSLKFRTPTLRNVALSGPYMHDGRFTTLQQCMDFFTDGVQAGPTL
                     DTLMTPDTGIPLTPQEKSDLIAFMGTLTDMSFIQDPKFKEQH"
     gene            complement(499..2697)
                     /locus_tag="VFU15_01630"
     CDS             complement(499..2697)
                     /locus_tag="VFU15_01630"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HEU4716496.1"
                     /translation="MKRYRIALFAVPAIIGLSTFIFLGSRCAAFPQPVKNNVKKDKAL
                     LSTMLKILDNNHYRPVTIDNDFSHKVYDTYLKRLDYRKQFFLQPDIDMLKNKFYDGID
                     DEIKGSTFEFFDSTISILNRRITEDKVYYTDALAKPFDFSVKESVQLDPEKCSYPADK
                     AAMKDSWRKYLKYQVMVRYVDAKKNQDKDLNDDKNLDKTKKSIIMMVDSAGTKIKKVK
                     PVKADSTLEREARMKVKKLEDDYFDRLTKAEHDDYMTSYLNVIVGIFDPHTEFFPPND
                     KENFDITMTGQLKGIGATLQEDNGNIKVTSIVPGSPSWKQGQLKPGDLILKVAQGDQE
                     PVDIVGKRVDDAVKLIRGEKGTEVRLTVQKPDGSIIVIPIIRDIVVLEETYAQSAMID
                     QDKKIGYIRLPGFYADFSRDHSSGRACSDDVKKELEKLKSENVKGVILDLRDNGGGSL
                     YEVIKMVGLFIPQGPVVQVKTLAQGIAPYGDDDNGKTVYDGPLVVLINDNSASASEIL
                     AAAIQDYHRGLIVGSSASTFGKGTVQNFYDLDDTYNGDDDFGKLGSLKVTIQKFYRIN
                     GGSTQLKGVTPDIELPGYYDFDKTGEKDLDYPMEWDQIPSAKYDMWKNEPAYGALQKA
                     SADRVAKSTYFALIRQKEEQLKRERDNTDESLFIDDYKAERQKIEDENKAFKPLEDPV
                     KGMNVSILSSDMKVLKGDTAMLARKNDFIDKVKKDPWISESAWIASDMKK"
     gene            complement(2817..3332)
                     /locus_tag="VFU15_01635"
     CDS             complement(2817..3332)
                     /locus_tag="VFU15_01635"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEU4716497.1"
                     /translation="MAEKRSNKKLWINAVTFALGAVLLVYLYIKYRVAPDIAFANLPV
                     ETLDGKPVSMTAYKGKGLLVNFWGTWCPSCVAEMPSMQEANAQLDTSRVMIIAISDEA
                     PSTISGFAEKKKYGFLFLHSKKSLQELGISTYPTSYLIDSNGKVILTKVGETDWAEPS
                     MVERLKALGGD"
     gene            complement(3358..5349)
                     /locus_tag="VFU15_01640"
     CDS             complement(3358..5349)
                     /locus_tag="VFU15_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEU4716498.1"
                     /translation="MTEIYEANREVTSKYVHATSRGHEAIQLAVGLQLKPQDFLSAYY
                     RDDSILLGIGMQPYELMLQLLAKRDDPFSGGRTYYSHPSLKRDGMPKIPMQSSATGMQ
                     AIPSTGVAMGIRYLEEEKLSADYGDEKPVVVCSLGDASITEGEVAEAFQMAVLKKLPI
                     LYLVQDNEWDISATSKETRAQDASEYARGFKGLETRLIDGTDFMRSYETVRDILAMIR
                     KERRPFLLHAKVPLLNHHTSGVRKEWYRNDLEEAARKDPFPRLRNQLIVSGIDPKDID
                     DIRVRADERVAEDYAKAVQAEDPRPEDLFNFDFAPTPVTEEKGVREPEGKEKTVMVDC
                     ALFAVQEILAKHPEALLYGQDVGGRLGGVFREAATLAQKFGDNRVFNTPIQEAFIIGS
                     TVGMSAAGCRPIVEVQFADYIWPGLNQLFTEVSRSYYLSNGKWPVSCVIRVPIGAYGS
                     GGPYHSSSVESVVCNIRGVKVAYPSTGADLKGLLKSAFYDPNPVVIFEHKGLYWSKIK
                     GTEDAKTIEPDEEYVVPFGKARMVLEAEASKNATTMTVITYGMGVYWAKNAAKNFPGR
                     VEILDLRTLVPVDEEAVFASVRKHSRCLVLTEEPYNNGFAQAISGRISKFCFESLDAP
                     VDIVGAENLPAIPLNSTLEHTMLPDAEKVAAAMEKLLAY"
     gene            5636..6214
                     /locus_tag="VFU15_01645"
     CDS             5636..6214
                     /locus_tag="VFU15_01645"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU4716499.1"
                     /translation="MSVETPTYLLIREKTARSVKILYERYGRKLYSYAVAKWNVDEDD
                     AWELVYKTLYRIKETAPGYEFESEQKFGAFVFTVFINYLRNFFRDRKDKTPETVSLKD
                     SHHAEAELPEETPPSPVMQLLRKELEKLDDWERMLLLMRNEDVPYAEIAKLTGKPADQ
                     LKVYYMRLKKKLAETLHEKIKLIQEENHENAG"
     gene            6198..>7473
                     /locus_tag="VFU15_01650"
     CDS             6198..>7473
                     /locus_tag="VFU15_01650"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HEU4716500.1"
                     /translation="MKTQDNFRFNEQTLDAALGLLLLEQEQLFTEKNTETMATHIFSA
                     TELLPVSSSKEKELLGRLGGSSGYTRFFSLPGLAIAILLATACIYYFFPHQPGKPQPH
                     TAAVSAAPGDSTGFRTTEKSPGKNVSAAVTVTVTPPAANSSPSPAVSQKDSVLAESES
                     ILPVTQDQLVYSHMPEDLYIGDDDIPVLTDAQKKKCAKEKLGMMKDVFRRKFPSYQPL
                     PGGTTSVNGKQRKVNGFYIASAEVTNLEYRTFLYDLLIQGREDDYVTARPVKGGWSAL
                     GIPEFEDIYWKSETYNDFPAVNITRKGAEMYCEWFTTSMKEAIANKDLKWKTGNPEFR
                     LPSNVEWIYAARGGDSTELKFPWGKFIPDSAQNHHGCFLCNFNYTASKDVFIDAHICP
                     GFSKLKQGGFHHAILTTAGMAIDTLVTAPVYSY"
BASE COUNT         1671 a         2030 c         1966 g         1806 t
ORIGIN      
        1 gaagcctcgc tcacctcaca ggaagccgac ggtcttgcaa ccttccgcac caaatgcgcg
       61 ggctgccacc ccgaaccgct tttcaccgac gaaggttttc acaacatcgg ccttcctgtc
      121 gacaacacgc tgaacgatga gggacgcatg cacatcacgc tcgatccggc cgattcgctg
      181 aaattcagaa cacctacgct gcgtaatgtt gcgctgagcg gaccctacat gcacgacgga
      241 aggttcacca cgctgcagca gtgcatggat ttcttcaccg atggcgtgca ggcaggaccg
      301 acgctcgata cgctgatgac gccggacacg ggaattccgc ttacgccgca ggaaaaatcg
      361 gaccttatcg cctttatggg aacgctcacg gacatgagct tcatccagga tccgaaattc
      421 aaggagcagc attgaccgtt cgaaaaaaaa aatcccggct cgtcgtgaac cgggattcgt
      481 tgttttgcct tccgcttttt attttttcat atccgaagcg atccaggcgc tttcgctgat
      541 ccacggatct ttcttcactt tgtcgatgaa atcatttttg cgtgcgagca tggccgtatc
      601 gcctttgagc actttcatgt cggaagaaag gatggaaacg ttcattcctt ttacgggatc
      661 ttcgagcggc ttgaatgctt tgttctcatc ttcgatcttc tggcgttccg ccttgtaatc
      721 gtcaatgaaa agcgattcgt cggtattgtc acgctcgcgt ttgagctgct cttctttctg
      781 cctgatgagc gcaaaatacg tgctcttcgc cacgcggtct gcactggctt tctgcagggc
      841 gccgtatgca ggctcgttct tccacatatc gtatttcgcg gaagggatct ggtcccattc
      901 catcgggtaa tcgagatctt tttcacccgt tttatcgaag tcgtaatagc ccgggagctc
      961 gatatccggc gtaacacctt tgagctgcgt ggagccgccg ttgatgcggt agaatttctg
     1021 gatcgtcacc ttgagagacc cgagctttcc gaaatcatcg tccccgttgt aggtgtcgtc
     1081 gagatcgtag aaattctgca cggttccttt tccgaatgtg gacgccgagc ttccgacgat
     1141 aagtccgcgg tggtaatcct gtatcgccgc cgcaagaatt tcggaagcgg atgcgctgtt
     1201 gtcgttgatg agcacgacga gcggcccgtc atacacggtt ttcccgttat catcatcacc
     1261 atacggggca atgccctgcg cgagcgtttt cacctgcacg accgggccct gcgggatgaa
     1321 aagtcccacc attttgatca cctcgtacaa tgatccgccg ccgttatcgc ggagatcgag
     1381 aataacgcct ttcacgtttt ccgacttcag cttttcgagc tctttcttca cgtcatccga
     1441 acaggcgcgg ccggagctgt gatcgcggct gaaatcggcg tagaacccgg gcaggcggat
     1501 gtagccgatc tttttgtcct ggtcgatcat cgcgctctgc gcgtaagttt cctccagcac
     1561 gacgatgtcg cggatgatcg ggatgacgat gatggaaccg tcgggtttct gaacggtaag
     1621 acgaacttcc gttccttttt cgccgcggat gagcttcacg gcatcatcca cgcgcttgcc
     1681 aacgatgtcc accggttcct ggtcaccctg cgcaacttta aggataaggt cgccgggctt
     1741 gagctgtccc tgcttccacg aaggacttcc gggcacaatg ctcgtgactt tgatatttcc
     1801 gttgtcttcc tgcagcgtcg caccaatgcc tttcagctgc ccggtcatgg tgatgtcgaa
     1861 attctccttg tcgttcggcg ggaaaaattc cgtgtgcgga tcgaagatgc cgacgatgac
     1921 gttcaggtag gaagtcatgt aatcgtcatg ttccgctttc gtcagccggt cgaaataatc
     1981 atcttccagc ttcttcactt tcatccttgc ttcccgttcc agtgtcgaat cggctttcac
     2041 cggtttcact tttttgatct tcgtccctgc gctgtcgacc atcatgatga tggacttttt
     2101 cgtcttgtcg aggttcttgt cgtcgttcag gtctttgtcc tggttctttt tcgcatcgac
     2161 ataacgcacc atcacctggt atttgaggta tttcctccag ctgtctttca tcgcggcttt
     2221 atcggccggg tacgaacatt tttccggatc gagctgaacg ctttccttca cggaaaaatc
     2281 gaacggtttc gcaagggcgt ccgtgtaata gactttgtct tcggtgatcc tgcggttcag
     2341 gatgctgatg gtgctgtcga aaaattcgaa cgtggatcct ttgatctcat cgtcaatgcc
     2401 gtcgtagaat ttatttttca gcatgtcgat atccggctga agaaagaact gcttccggta
     2461 gtcgagacgt ttgagatacg tgtcgtacac tttgtgcgaa aaatcattat cgatcgtgac
     2521 cggacggtaa tgattattat ccaggatctt cagcatggtg ctgaggagcg ccttgtcctt
     2581 tttaacattg ttcttcacgg gctggggaaa tgcagcgcag cggcttccca ggaaaatgaa
     2641 ggtcgacagg ccaatgatgg ccggcacggc aaaaagggct attctgtaac gtttcataat
     2701 cttcttttag taataacaac gaaattactc aaaaagcatg cgaaaacgag ttaagatgtg
     2761 ttaagcactt tacgagtggg cggccatgag gatgaacgga aatcgtcccc gaaattctaa
     2821 tcgccgccga gcgctttcag ccgttccacc atcgacggtt cggcccagtc ggtttcgccg
     2881 accttcgtca ggatcacctt cccgttgctg tcgatgaggt agctggtggg gtacgtgctt
     2941 atcccgagct cctgcaagga ttttttactg tgcaggaaca ggaagccgta tttttttttc
     3001 tctgcgaaac cggaaatggt ggaaggcgcc tcatccgaga tcgcaatgat catcacccgc
     3061 gacgtgtcga gctgcgcgtt cgcttcctgc atcgaaggca tttccgcaac acaggacggg
     3121 caccacgttc cccagaaatt gacaagcaat cctttgcctt tgtaagcggt cattgaaacc
     3181 ggtttcccgt cgagtgtttc cacgggaaga tttgcaaagg cgatgtcagg cgcaacgcgg
     3241 tatttgatgt acagataaac aaggagcacc gcaccaagtg caaacgtgac cgcgttgatc
     3301 cagagttttt tgttgctgcg cttttccgcc aaaacaggtc gaccggttaa attactttca
     3361 gtatgccagc agcttttcca tggcagccgc taccttttcc gcatcgggaa gcatggtatg
     3421 ctcgagcgtg gagttgagcg ggatggccgg gaggttttcc gcgccgacga tatcgacagg
     3481 tgcgtccagc gattcgaagc agaattttga aatacgcccg gagatcgcct gtgcgaaacc
     3541 attgttgtac ggctcttccg tcagcacgag gcagcgcgaa tgtttccgca cggaagcgaa
     3601 cacagcttct tcatcgaccg ggaccagcgt gcggagatcg aggatctcga cgcggccggg
     3661 gaaatttttc gcggcatttt tcgcccagta aacgcccatg ccgtaagtga tgacggtcat
     3721 cgtggttgca ttcttcgaag cttccgcttc cagcaccatt ctcgctttcc cgaagggaac
     3781 cacgtattct tcgtcaggtt cgatcgtttt cgcgtcctcg gttcctttga tcttcgacca
     3841 gtacaatccc ttgtgctcga agatcaccac cgggttcgga tcgtagaacg ccgatttcag
     3901 caatcctttc aggtcagcgc cggtggaagg atacgcgact ttcacaccgc ggatgttgca
     3961 caccaccgac tcgacgctgg agctgtgata aggtccgccg ctgccgtatg caccgatggg
     4021 cacacgtatc acgcagctca ccggccattt tccattggag agataatatg aacggctcac
     4081 ctcggtgaac agctggttga ggccgggcca gatataatcg gcgaactgaa cttccacgat
     4141 cgggcggcaa cccgcggcag acataccgac ggtggagcca atgatgaatg cttcctgtat
     4201 gggtgtattg aaaacgcggt tgtcgccgaa tttctgtgcg agggtcgccg cttcgcggaa
     4261 cacgccgccg agccttcctc ccacgtcctg cccgtacagc aaggcttccg ggtgcttcgc
     4321 aagaatttcc tgcacggcga acagcgcgca atccaccatc actgtttttt cttttccttc
     4381 cggctcgcgc acaccttttt cctccgtcac cggcgtcggt gcaaaatcga aattgaaaag
     4441 atcttcgggg cggggatctt ccgcctgtac agcttttgcg taatcttccg ccacgcgttc
     4501 gtctgcacgc accctgatgt catcgatatc tttcggatcg atgccggaaa cgatcagctg
     4561 gttgcgcagc ctgggaaaag gatctttccg cgctgcttct tcgagatcgt tgcggtacca
     4621 ttctttccgg acgccggatg tatggtgatt gagcagcgga acctttgcat gcagcaggaa
     4681 cggcctgcgt tctttgcgga tcatcgccag gatatcacgc accgtttcgt agctgcgcat
     4741 gaaatcggtt ccgtcgatga gacgcgtttc gagtcctttg aacccgcgcg cgtattcaga
     4801 cgcatcctgc gcacgtgttt cctttgaagt ggcggaaatg tcccattcat tgtcctgcac
     4861 gagatacagg atgggcagct ttttcagcac cgccatctgg aatgcttcag ccacttcgcc
     4921 ttccgtgatc gacgcatcgc cgagagagca gacgacgacc ggtttttcat cgccgtaatc
     4981 ggcggagagc ttttcctctt cgagataacg tatgcccatg gcgacgccgg tgctggggat
     5041 cgcctgcatt cccgtggccg agctttgcat ggggatcttc ggcatcccgt cgcgcttcag
     5101 gctcgggtgg ctgtaataag ttcttccgcc ggaaaacgga tcatcgcgct tggcgagcag
     5161 ctgcagcatg agctcgtacg gctgcatacc gatgccgagc aggatgctgt cgtcgcggta
     5221 atacgcggaa aggaaatcct gcggtttgag ctgcagtccg acggcgagct ggatggcttc
     5281 gtggccgcgt gaagtggcgt gcacatattt cgaagtgacc tcgcggttcg cttcgtagat
     5341 ctcggtcatg gctttggcgg tgcacatcag ttcccaggcc ctgagggcta ctgaaaccgg
     5401 aacgcctcct ggttttgcac gttgttcctg cacggagaga gacataatgc gttatgaaaa
     5461 tgtgaatcgt gtgaataacg aagatactga aaagtctgac gggatacagg ttcccggttc
     5521 cgggttcaaa gttccgggtt ccaaactcaa agcaacaacc gaaaagcgcg aagtgaaaac
     5581 cggaaaacgg ccggactgtt acctttccgt gttcttatat ccggaagcga agaacatgag
     5641 cgtcgaaaca cccacatatc tcctgatcag ggaaaaaacg gcgaggtcgg taaagatcct
     5701 gtacgaacgt tacggacgaa agctgtattc gtatgcggtc gcaaaatgga acgtggatga
     5761 agatgatgcg tgggagctgg tgtacaaaac gctttaccgc ataaaggaaa cggcgccggg
     5821 ttatgaattt gaatccgaac agaaattcgg tgctttcgtc ttcacggtgt ttatcaatta
     5881 cctgcgcaat tttttccgcg acaggaaaga caaaacaccc gaaaccgttt cgctgaagga
     5941 ctcgcatcac gccgaagcgg agctgccgga agaaacacct ccttcgccgg tgatgcagct
     6001 gctgcggaaa gagctggaaa agctggacga ctgggaacgc atgctgttgc tgatgcgaaa
     6061 cgaggacgtc ccgtatgcgg agatcgcgaa gcttaccgga aaaccggcgg accagctgaa
     6121 agtgtattac atgcggctga aaaagaaact ggctgaaacg ctccacgaaa aaatcaaact
     6181 tatccaagag gaaaaccatg aaaacgcagg ataatttccg cttcaacgaa caaacgcttg
     6241 atgcagcgct cgggttactc ctcctggagc aggaacagct attcaccgaa aaaaacacag
     6301 aaaccatggc tactcatatt ttctctgcaa cagaattact cccggtttct tcttccaaag
     6361 aaaaagagct cctgggacga ctgggcggca gcagcggcta tacccgcttc ttcagcctgc
     6421 ccggtcttgc gatcgccatc cttctcgcga cggcctgcat ttattatttc ttcccgcatc
     6481 aaccgggaaa accacagccg cacacggccg cagtatccgc agctccgggc gactccaccg
     6541 gcttccgtac aaccgagaaa agcccgggga aaaatgtctc cgctgctgtt accgtgacgg
     6601 tcaccccgcc ggcggcgaat tcctctccgt cgcccgccgt gtcgcagaag gattctgtcc
     6661 tggccgaaag cgaatccatt cttcccgtga cgcaggatca gctcgtttat tcacatatgc
     6721 cggaagatct gtatatcggc gacgacgata ttcccgtgct taccgacgca caaaagaaaa
     6781 aatgtgcaaa ggaaaagctg ggcatgatga aagatgtctt ccgccggaaa ttcccgtctt
     6841 accagccgct tccgggagga actacttccg taaacgggaa acagcggaaa gtgaacggtt
     6901 tttacatcgc aagtgcggaa gtgaccaacc tcgaataccg gacgttcctg tatgaccttc
     6961 ttatccaggg acgcgaggat gattacgtga cggcacggcc cgtgaaaggc ggctggagcg
     7021 ccctcgggat cccggagttc gaggacattt actggaagag cgaaacctat aatgatttcc
     7081 cggcggtgaa catcacgcgc aaaggagccg aaatgtactg cgagtggttc acaacatcca
     7141 tgaaggaagc catcgccaat aaagacctga aatggaaaac gggcaacccg gaattccgtt
     7201 tgccgagcaa tgtcgaatgg atctacgccg cacgcggagg agattcgacg gaactgaaat
     7261 tcccgtgggg aaaattcatc ccggacagcg cgcaaaatca ccacggctgc ttcctctgca
     7321 attttaatta tacagcttcg aaagatgtct tcatcgatgc gcatatctgt cccggctttt
     7381 cgaaactgaa gcagggagga ttccatcacg ccatcctcac cacggccggc atggcgatcg
     7441 atacgctggt cacagcacct gtatattcct aca
//
LOCUS       DASTSF010000053         2450 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1973699,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000053 DASTSF010000000
VERSION     DASTSF010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2450
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1973699"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..838)
                     /locus_tag="VFU15_01655"
     CDS             complement(<1..838)
                     /locus_tag="VFU15_01655"
                     /inference="COORDINATES: protein motif:HMM:NF025644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHHW family protein"
                     /protein_id="HEU4716501.1"
                     /translation="MKFNNRRFYSRLNIFLFSGLLIVLGVRFFFGEHKKFSEDEKRDL
                     AVMPSFTWNTFFDGKYIDSIDDYIADHFPYRDEFLKTSFTMRDSRGIKDNGISYYRKP
                     VVRKEPQNKVDVKKSMTADTAAAADTTAEEPDGAEEEDNLLLYNGHAMEIFGGNDKMA
                     KAYADVINSYQDALKGKVKIYDVVVPSSIEFNCPPGYKTNEKRNIGAIYNNLAPGVTG
                     VDAYSAIAPHRNEYIYFRTDHHWTGLGAYYAYTATARDMGFTPLELDQLDKRYIPNFL
                     GSL"
     gene            complement(884..2293)
                     /locus_tag="VFU15_01660"
     CDS             complement(884..2293)
                     /locus_tag="VFU15_01660"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002656552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HEU4716502.1"
                     /translation="MVFASLVFLYIFLPLNLILYYALKDSVYRNALLTIFSLFFYAWG
                     EPVWVFLLIFTSLVDYANALFIEKYRDTKWAKAGLVSSVIFNLGILMVFKYSGFFVQN
                     LNFITGLSIPVPAFTLPIGISFYTFQSVSYVVDVYRREVPAQRDYMKFLMFVSLYHQL
                     VAGPIVRYVHIANEIDSRKFNLKDISAGVTRFCIGLFKKVCIANVAGQFVATYLDGDL
                     TKITGAEAWFGILMYTFQIYFDFSGYSDMAIGLGRMFGFHYYENFNYPYIANTATEFW
                     RRWHISLSTWFRDYIYIPLGGKYKFQVRNLFIVWFLTGFWHGASWNFILWGLYWGTLI
                     FLERMFLFKVLEKLPKFIWHLYLLFAVVIGWTLFYFTDFERLGQFMNILFGLAPNTFW
                     TMPLQVVLKENMFWIAITVILCTPVYRFINWWTKDFLGRRKWHLLWYGTIAINVVLLL
                     LSTAMLVEKSYNPFIYYRF"
BASE COUNT          623 a          588 c          665 g          574 t
ORIGIN      
        1 aaagcgagcc gaggaaattc gggatgtagc gtttgtccag ctgatcgagc tccagcggag
       61 tgaatcccat gtcgcgcgct gttgccgtgt aagcataata cgcgccgagt cctgtccagt
      121 gatggtccgt gcggaaataa atgtattcgt tcctgtgcgg cgcaatggcc gaatacgcat
      181 ccacgccggt aacgccgggc gcaagattgt tgtagatggc gccgatgttc cgcttttcgt
      241 tcgtcttgta gccgggcggg caattgaatt cgatggagga gggaacgacc acatcgtaga
      301 tcttcacttt tcctttcagc gcatcctggt agctgttgat cacatcggcg tacgctttcg
      361 ccattttatc attgcccccg aaaatttcca tcgcgtgacc gttgtacagg aggaggttgt
      421 cttcttcttc tgctccgtca ggctcttcgg ccgtggtgtc tgctgcggca gcggtatcgg
      481 cggtcatgga ctttttcaca tccactttgt tctgcggctc tttccgaaca acaggttttc
      541 ggtaatagga aatgccgttg tcctttatcc cgcggctgtc gcgcatcgtg aaggaggttt
      601 tcaggaactc atcgcggtaa gggaaatgat cggcaatgta atcgtcgatg ctgtcgatgt
      661 attttccgtc gaagaatgta ttccacgtga acgacggcat cacggcgaga tcgcgctttt
      721 cgtcttccga gaatttttta tgctcgccga aaaagaaccg caccccgagc acgatgagca
      781 gtcccgagaa caggaaaatg ttcagccttg aataaaatct cctgttgttg aatttcattt
      841 cagcggtgat tttgctgtcg attaaagtta gtcaattcgt ttttcagaag cggtaataaa
      901 tgaacgggtt gtagctcttc tccaccagca tcgcagtgga aagcagcagc agcacgacgt
      961 tgatggcgat cgttccgtac cacagcaggt gccatttccg gcgaccgaga aaatccttcg
     1021 tccaccagtt gatgaaacgg taaaccggcg tgcagagaat aaccgttatc gcgatccaga
     1081 acatgttttc cttcagcacc acctgcagcg gcatcgtcca gaacgtattc ggcgcaaggc
     1141 cgaagaggat gttcataaac tgcccgagac gttcgaaatc ggtaaagtag aaaagcgtcc
     1201 agccgatgac gaccgcgaac aggaggtaca ggtgccagat gaatttggga agtttttcca
     1261 gcactttgaa caggaacatg cgttcgagaa agatcagcgt tccccagtat aatccccaca
     1321 gaatgaaatt ccagcttgcg ccgtgccaga agccggtgag aaaccaaacg atgaagagat
     1381 tcctgacctg gaatttatat tttccgccga gcgggatgta gatgtaatcg cggaaccagg
     1441 tggaaagcga aatgtgccag cgccgccaga attccgttgc ggtgttggcg atgtacgggt
     1501 aattgaaatt ttcataatag tggaacccga acatccggcc caggccgatc gccatgtcgg
     1561 aatagcccga aaaatcgaaa tagatctgga acgtgtacat caggatgccg aaccacgctt
     1621 ccgcgcccgt gatcttcgtg agatcgccgt cgagatacgt tgccacgaat tgtcccgcca
     1681 cgttggcaat gcacactttt ttgaaaagcc cgatgcagaa acgggtgacg cccgcgctga
     1741 tgtccttgag gttgaatttc cgcgagtcga tctcgttcgc gatgtgcaca taacgtacaa
     1801 ttggtccggc aacgagctgg tggtagaggc tcacgaacat caggaacttc atgtagtcgc
     1861 gctgcgcggg aacttccctc cggtaaacat cgacgacata ggaaaccgac tggaacgtgt
     1921 agaaggaaat cccgatggga agcgtgaatg cgggaaccgg gatggaaagg ccggtgatga
     1981 aattcaggtt ctgcacgaaa aacccgctgt acttgaacac catcaggatg ccgagattga
     2041 agatcacgga agaaacaagt cctgccttcg cccatttcgt gtcgcggtat ttctcaatga
     2101 aaagtgcatt ggcgtaatcc accagcgaag tgaagatgag caggaacacc cacacgggtt
     2161 cgccccaggc gtaaaagaaa agggaaaaaa tggtcagcag cgcattcctg taaacgctgt
     2221 cctttaatgc gtaatacagg atgaggttaa gcggcaggaa aatgtagagg aaaacaagac
     2281 tggcaaaaac catacgtcag gcagaaattt agcgggtggg ttcggtcatg gcttttatcc
     2341 gttccaaaaa tagcagtttg tccctgcatc ctttacggca ggtgataatt gatgtgttat
     2401 ttcgaataaa tggcgagatc gggaatggaa gtgatccggc atccgggttc
//
LOCUS       DASTSF010000054         4446 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1078053,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000054 DASTSF010000000
VERSION     DASTSF010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4446
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1078053"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..463)
                     /locus_tag="VFU15_01665"
     CDS             complement(<1..463)
                     /locus_tag="VFU15_01665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716503.1"
                     /translation="MNPDALIDRLIYPGDRSDPDSYRRGKFFLISVFIFFDLNLLAGI
                     FYGVSHHSARDVLFITLSIVPLLIPFLVYPKYGRRVLLVNVLAFLGTFFTLPFTYKYS
                     GGIFASDFVILIVTSAWIFLSAGKISGYVWTSLNLAIILFFWYAANAGWKNF"
     gene            561..2666
                     /gene="recG"
                     /locus_tag="VFU15_01670"
     CDS             561..2666
                     /gene="recG"
                     /locus_tag="VFU15_01670"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009197491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEU4716504.1"
                     /translation="MAESLLETPVEYLKGVGPERAKVLKAEFGIHTFGDLLRWYPYRY
                     VDRTKFYSTAEINADMPFVQLRGHILHPQVVGQKQGKRMTAEFTDAHGSVELVWFQGL
                     KWLQGKFLPGKEYIVFGRPSVYNGKITIPHPEVEEVKEENTKRASALQPMYSSSEKAK
                     MKGLDTKGILKLMKVLVQMAEGKIGETLPAGVTGKFKMLSRAEALAQIHFPSDHHLLK
                     RAQARIKFEEFLYVQLRLLRVKKMRQQNLRGFNFEKVGEKFNTFYNKYLPFPLTEAQK
                     RVIREIRADIGSGKQMNRLLQGDVGSGKTVVALMCMLIANDNGYQACIMAPTEILAVQ
                     HYQTLKELLKDMPVQVGLLTGNTKRPERKKLHERLRSGELDILIGTHALIEDEVQFMN
                     LGLVVIDEQHRFGVEQRSKLWRKNENPPHILVMTATPIPRTLAMTLYGDLDSSVINEL
                     PPGRKPIRTVHFYDSQRLRVFGFMKEEIEKGRQIYVVYPLIEESEKLDYKDLMDGYES
                     ISRAFPLPQYRVSIVHGRMKPADKDFEMQRFVKGETNIMVATTVIEVGVNVPNASVMV
                     IESAERFGLSQLHQLRGRVGRGAEQSYCILMTGNKLGRESKLRMDTMVRTNDGFEIAE
                     VDLKLRGPGDIEGTQQSGLTGLRLADISRDAPILQHARTVAAEILDNDPMLSSEENAP
                     MVKEMNREKSSSVNWGRIS"
     gene            complement(2929..>4446)
                     /locus_tag="VFU15_01675"
     CDS             complement(2929..>4446)
                     /locus_tag="VFU15_01675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716505.1"
                     /translation="DSFVVSLLFLFTGFAIIIYLNQTSWQPRERDYAYVGSFYAFAAW
                     IGLGVIAVFNGLKNFLTAKTALIVASSVSALSPLLLYTQNLDDHNRHGRTVARDVAVD
                     FLESCAPNAILFTYADNDTFPLWYAQEVLGVRRDVRIVCLSLLRSDWYIDQAKKKQYT
                     SDPLPVSWDHWSYRDGTRDYMNIFEDSEDTLDLRYVDEKMTSADPADRFLSTYGDTLN
                     YIPTRNVFLPVNKKEFMKSSTLEASQEKNIADTVFFRLRGNYLLKDQEIVLDILAHND
                     WKRPVYFAMNMPLTCYAGLDDYLQLEGLAYRFVPVKNPRPDGDFQSRPLVNLQKCYDL
                     VMNKFGWGGISDLSVYADETSQRMFSDYLRGSCSMIATALAEEGKSKEALGVIRKCVS
                     GIPGEQDAPDLAWDEMVNAAYLAGDAALAEKLAEEGFDEYERCVIWYGTLPRLPGDFS
                     DKKNGMFTLADYAMNYGDSTLYNDFCRRMKAFRFQVPVPDAETDDNDSVTDDSMK"
BASE COUNT         1078 a         1120 c         1311 g          937 t
ORIGIN      
        1 ggaaattttt ccatcctgca ttagcggcgt accagaaaaa caggatgata gcgaggttca
       61 gcgaagtcca tacgtaaccg ctgatcttgc cggcgctcag gaagatccac gcagaggtga
      121 cgatgaggat gacgaaatcg ctggcgaaaa ttccgcccga atatttgtat gtgaacggca
      181 atgtgaaaaa agtgccgaga aacgcgagca cattcacgag cagaacgcgg cgcccgtact
      241 tcgggtatac caggaacgga atgagaagcg gaacgatgga gagggtgatg aagagcacat
      301 cgcgtgcgga gtggtgtgac acaccgtaaa aaattccggc aagcaggttg aggtcgaaaa
      361 aaatgaagac cgagatgagg aagaattttc cgcggcggta agaatccggg tcgctgcgat
      421 cgccgggata gatcagcctg tcgataagcg cgtcgggatt caagagcgga tgcgtttttt
      481 agtgtgcagc gctaatatac gaatgcggga atacacggcc ttttttcggg attcgtatct
      541 tcgttccatt catccttgcc gtggcggaaa gccttctcga aacaccggtc gaatatctca
      601 aaggcgttgg tcctgaacgt gcgaaagtcc ttaaagcgga attcggcatt cacaccttcg
      661 gcgatctttt gcgctggtac ccgtaccgct atgtcgaccg gacgaaattc tacagcaccg
      721 cggaaataaa cgccgacatg cctttcgtgc agctgcgcgg gcacattctt catccgcagg
      781 tagtggggca gaaacagggg aaacggatga cggctgaatt cacggatgcg cacggaagcg
      841 tggagctagt ctggttccag ggattgaaat ggctgcaggg gaaattcctt cccgggaaag
      901 aatacatcgt gttcggacgt ccttccgtgt acaacgggaa aataacgatc ccgcacccgg
      961 aggtggagga agtgaaagaa gaaaatacga aacgtgcgag cgcgctgcag cccatgtatt
     1021 cttcttcgga aaaggcgaag atgaaagggc tcgacacgaa aggaattctc aagctgatga
     1081 aagtgctcgt gcaaatggcc gaagggaaga tcggggaaac gcttccggcc ggcgtcaccg
     1141 gtaaattcaa aatgctttca cgcgcggagg cgctggcgca gatccatttt ccttctgacc
     1201 atcacctgct gaaacgcgca caggcgagaa taaaattcga agagttcctg tacgtgcagc
     1261 tgcggctgct gcgggtaaag aaaatgcgcc agcagaatct gcgcgggttc aattttgaaa
     1321 aggtcggcga gaagttcaac acattttaca acaaatacct tcccttcccg ctgacggaag
     1381 cgcagaagcg tgtgatccgt gagatccgcg ccgatatcgg ttccgggaaa cagatgaacc
     1441 gcctgctgca gggcgatgtg ggcagcggga aaacggtggt cgcgctgatg tgcatgctca
     1501 tcgcgaacga caatggttac caggcgtgca tcatggcgcc cacggaaatt ctcgccgtgc
     1561 agcattacca gacgctgaag gaattgctga aggacatgcc ggtgcaggtc gggctgctga
     1621 ccgggaacac gaagcggccg gagcggaaaa agctgcatga gcggctgcgt tcgggcgaac
     1681 tggatattct tatcggcacg catgcgctca ttgaggacga agtgcagttc atgaacctgg
     1741 gtctggtggt gatcgacgag cagcaccgtt ttggcgtgga gcagcggtcg aagctctggc
     1801 gcaagaatga aaatccgccg catattctcg tcatgacggc aacgccgatc ccgcggacgc
     1861 tcgcgatgac gctgtacggc gatctcgatt cttccgtcat caacgaattg ccgccgggaa
     1921 gaaaaccgat ccgcacggtg catttttacg attcgcagcg attgcgcgtg ttcggtttta
     1981 tgaaggaaga aattgaaaaa gggagacaga tctacgtggt ttacccgctc attgaagaaa
     2041 gcgagaagct ggattacaaa gatctcatgg acggatatga aagcatttcg cgcgcgttcc
     2101 cgctcccgca ataccgcgtc agcatcgtgc acggacgcat gaagccggcc gacaaggatt
     2161 tcgagatgca gcgtttcgta aaaggagaaa cgaacatcat ggtcgcgaca acggtgatcg
     2221 aagtcggcgt gaacgtcccg aatgcgtcgg tgatggtgat cgaaagcgca gaacgtttcg
     2281 gtctttccca attgcaccag ctgcggggcc gcgtgggaag aggcgcggag cagtcgtact
     2341 gcatcctcat gacgggcaac aagctcggca gggaatcgaa gctgcgcatg gatacgatgg
     2401 tgcgcacgaa tgacggtttt gaaattgcgg aagtcgacct gaagctacgc ggaccgggcg
     2461 acatcgaagg cacgcagcag agcgggctca ccggcctgcg tctcgcggat atttcgaggg
     2521 acgccccgat tcttcagcac gcgagaacgg tcgctgcgga aatcctcgat aacgacccga
     2581 tgctgtcgtc ggaagaaaac gcgccgatgg tgaaagagat gaaccgggaa aagagcagct
     2641 cagtgaactg ggggaggatt tcgtaagaga agttgggaag tttcgggttg ggagtttagg
     2701 gttgttttcc gttcccgcca ggtcgcggaa gaaaatcatt ttccgttcgg gtgaagtctg
     2761 tccgttcggg cgaagtctgt ccgttagggc gaagtctgtc cgttcgagcg aagtctgtcc
     2821 gttcgggcga agtctgtcag ttcgagcgaa gccgagaact gtttcagtgc attattcgtt
     2881 cttcccccct tttaaatttt tctgtttggg attcggaaaa taaatctctc acttcatcga
     2941 atcatctgtc acggaatcgt tgtcatctgt ttctgcatcc ggtacgggca cctggaagcg
     3001 gaacgctttc atccgccggc agaaatcatt gtacagcgtg gaatctccat agttcatcgc
     3061 gtaatcggcg agcgtgaaca tgccattctt cttatcggag aaatcacccg gcaggcgcgg
     3121 aagcgttcca taccagatga cgcatcgctc gtattcatcg aaaccttctt ctgccaattt
     3181 ctccgcgagc gcagcatctc ctgccaggta agccgcgttc accatttcat cccacgcaag
     3241 atccggcgca tcctgctccc cgggaatccc ggaaacacat ttgcgaatga cgcccagcgc
     3301 ttcttttgat ttcccttctt ccgccagcgc tgtcgcgatc atcgagcagg aaccgcgcag
     3361 gtaatcggag aacatgcgct gcgacgtttc atcggcgtaa acggaaaggt cgctgatgcc
     3421 gccccagccg aatttattca tgacgaggtc gtagcacttc tgcaggttca ccagcgggcg
     3481 cgactggaaa tctccgtccg ggcgcggatt ttttaccggg acgaaacggt aagcaagacc
     3541 ttcgagctgc aggtaatcat cgagacccgc atagcaggtg aggggcatgt tcatcgcgaa
     3601 atacaccggg cgtttccagt cgttatgcgc cagtatgtcg agcacgatct cctggtcttt
     3661 cagcaggtaa tttccccgca ggcggaagaa gaccgtgtcc gcgatatttt tttcctgcga
     3721 cgcttcgagc gtgctgctct tcatgaattc ttttttattc accggcagga aaacgttccg
     3781 cgtgggaatg taattcagcg tgtcgccgta agtcgaaagg aaacggtcgg ccggatccgc
     3841 gctcgtcatt ttttcatcca cgtaacgcag gtcgagcgtg tcttccgaat cttcgaagat
     3901 gttcatgtaa tcgcgtgttc cgtcgcggta gctccagtga tcccagctca cgggcagcgg
     3961 atcggacgtg tactgtttct ttttcgcctg gtcgatgtac caatcactgc gcaggagcga
     4021 aaggcagaca atgcgcacgt cgcgccgcac accgagcact tcctgcgcat accagagcgg
     4081 gaacgtgtcg ttgtcagcat aggtgaaaag aatggcgttg ggtgcgcagc tttcgaggaa
     4141 atcgacggcc acgtcgcgcg cgaccgttct tccgtgccgg ttgtgatcat cgaggttctg
     4201 cgtgtagagc agcagcggtg acaatgcgct gacggatgaa gcgacgatca gcgccgtttt
     4261 cgcggtaagg aaattcttca ggccgttgaa tacggcgatc acgcccaggc cgatccaggc
     4321 agcgaacgca tagaaagaac cgacatacgc gtaatcgcgt tcacgcggtt gccacgatgt
     4381 ctggttgagg taaatgataa tggcgaaacc cgtgaagaga aaaagaagcg aaaccacgaa
     4441 ggagtc
//
LOCUS       DASTSF010000055         7115 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_634858,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000055 DASTSF010000000
VERSION     DASTSF010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 7115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7115
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_634858"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1513
                     /locus_tag="VFU15_01680"
     CDS             <1..1513
                     /locus_tag="VFU15_01680"
                     /inference="COORDINATES: protein motif:HMM:NF040192.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEU4716506.1"
                     /translation="STYGGRLWKYNSSMVSQWVKTAGTRLNGVDADNSGNSYVTGTPP
                     GGGIFVQKYNSAGTAQFGTGYSIVEANADAKEIHVSGGDIYLGGTFYNTANFGFDYDV
                     ISLAGETSSSVFWAKYQVDNTAPDVTLTTPVSSPTNQDPVPVTITFSEPVNGFNPNPG
                     DPNNATLGNVVQVNATTFTADLIPSVSSGQIDYTVINGLCTDNHNNYNNSSNTLIIDY
                     DAVAPTVSVSSTAPDPTNMSPIPVTFTFSENVSGFTAGDIVVANGTASNFISVSGSIY
                     TADITPASGGNVSVQVNGNAAQDGASNGNTASNIFSIVYDDVHPAVVIMSTAPDPTST
                     SPIPVTFTFSENVSGFTVGDIAVTNGTASNFVAVSGTVYTADILPSAQGLVSVDVPAA
                     VCADGATNPNDAATQFSITYQLTTGISGPAGIQPFTVFPNPCTGSFTVRLPGGWTAGT
                     VSVYDVTGRMCFSAGTVTGDHVTLQTGLPAGCYSVEIVSPQSRTILPLIIDQH"
     gene            1524..2147
                     /locus_tag="VFU15_01685"
     CDS             1524..2147
                     /locus_tag="VFU15_01685"
                     /inference="COORDINATES: protein motif:HMM:NF023145.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tudor-knot domain-containing protein"
                     /protein_id="HEU4716507.1"
                     /translation="MKRKLLVFSSAAALCFFLFSCGGKKENAPANDPGPSSQTSTAPA
                     ANVRPDWLSSSSGKLWTPDKGDAPFSKMTFYSDGTLQDAVSKSKWKINGNQLIVNIGF
                     DISYTIAKVSDNSFSLETGKGKTFVYTAASADAGSPNDAQPVPSSSAPADAAPSFSKG
                     DKVQVNWKGSWFPATILEVKADGKYRIHYTGYDSSWDETVSSSRIKK"
     gene            2187..2489
                     /locus_tag="VFU15_01690"
     CDS             2187..2489
                     /locus_tag="VFU15_01690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716508.1"
                     /translation="MKKNAILFTATLVLAAAFIYACGGKKADHGKWTDQDIDNAKKNC
                     MFGKDPALTDDQQKAICDCWVDKVMAASPDPAEQSKIPMDEVNKMTTDCRASAGVK"
     gene            complement(2516..4306)
                     /locus_tag="VFU15_01695"
     CDS             complement(2516..4306)
                     /locus_tag="VFU15_01695"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEU4716509.1"
                     /translation="MRRNVPLLCFFLLSVNACLWGQASKMLDRAKEFSKNGSTDSARS
                     CFTKAVSEAIDEKDPALVCKTYFAFGDFIYYQGQIDESATQYFNALNVARSNRLVKQE
                     LLALRDLGWCYRELGRNEQAAQYYYEALSIAFQNKDYKEMAYCHSYIFTMYDVAGKND
                     SSLVHLHELEKYTKLTGDSDLLYLTNTNYGLYYYDTKNYGEAIRYFDICRRFPRLNND
                     PQELLQYYNRYGNSLKHLGRVKEGIVYLDSARAYAEQHNDLTQQWYAYLNLSDAYYDA
                     GMYREAMDYLRKYNDINDSIESVDASEKMATMQAKYDVQSREQKIKLLSQAREAEAQK
                     LNFIIGVSVLGFILLVISVFAFFNTRRKNSELRMQKAEIENKTEELNRQAAEIARFRT
                     QMNPHFVFNAVNSLQRFILTNEKEKALAAIADFSSLMRQTLYNSDKSVITLQEEIAFL
                     EKYVLFEQFRFENKFSFRVETAGGIQPDDTFIPPMIIQPLVENAVRHGVVPLTGPGEI
                     VLRFSVEGRTLVVSVRDNGAGRSASEKMKNASSMHESKGTSITRIRVAQFCRMNGADE
                     KDSFVISDLKNANGSAAGTEVILRLPLVSH"
     gene            4396..5166
                     /locus_tag="VFU15_01700"
     CDS             4396..5166
                     /locus_tag="VFU15_01700"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU4716510.1"
                     /translation="MSTPGKLSVVIITFNEEKNIGRCLDSVKDIADDIVVLDSFSSDN
                     TKKICEEKGARFFQHAFDGHIEQKNRAITFAAYPFVLSLDADEAPDEELKRAISEVKK
                     NKTADGYSMNRLTNYCGKWIRHCGWYPDTKLRLWDSTKGKWGGVNPHDKYEMTAAGAT
                     VKHLPGNILHYSYYSVDEHRQQAEKFSTIAAKALGQSGKSSGIFLALVKTAAKFIRNY
                     FIKLGFLDGKYGFIICRISAWETWMKYDKLRRMKKENA"
     gene            5163..6302
                     /locus_tag="VFU15_01705"
     CDS             5163..6302
                     /locus_tag="VFU15_01705"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HEU4716511.1"
                     /translation="MNRLFEKYPFLCSKKFLAALYAVVVTAATLHRLWLGESSLNNFT
                     IFRWSFFNLAHGNDLYAYHPDQYNDLFKYSPTFAFFMAPFWWLPKAAGLFAWNFINAF
                     VPFFAVQRLKITDRAKAFILLFAGIELLGSIQNCQSNGLMAGLIIFAYAMMEEQKMWQ
                     SALFLCLGFYVKIFAVVAGIFFLFSERKMQFLAWGILIFAALGLLPASVCGFGGLITQ
                     YKSWFHLIANDPAHELNYSFMTLTQRWFSFTAPDSVYLVPGMILLLLPLVRKKMWKED
                     NFRLLLLASVLLWVVIFNHKAESPTYVIAVFGAGIFGAAEKPSVPRTFLLWFVFILTV
                     LAATDLMPPFVRDEIIKPYCLKALPCIVLWLVVQWKLLRQDFSAA"
     gene            complement(6308..>7115)
                     /locus_tag="VFU15_01710"
     CDS             complement(6308..>7115)
                     /locus_tag="VFU15_01710"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEU4716512.1"
                     /translation="HPYTHQVNYPMLENGNQVMINICWFELDSCWNARSRIVLNGGKD
                     KKGNPISITLIRYLSSKGWKKDADAMSKLTDAEIHAIENGKTNALDSTRSPFGRRIYQ
                     VMWEIYHYLHGANPSGNSVTMRIELFKTSLTCSREHLLFGVGTGGQEKAFANCYQING
                     TMLDEDWRWMHSHNQFLSSLVTLGIPGLIFFLLMLFYPASSMKRWGSFLFLAFFIVLF
                     LSFIDEDTLETSQGVIFFAFFNSLFLFAMPWGSAVRQKEMKELPEEKQGA"
BASE COUNT         1655 a         1905 c         1877 g         1678 t
ORIGIN      
        1 gagcacgtat ggcggcaggt tgtggaaata caattcctcg atggtttcgc aatgggtgaa
       61 aacagccggc acgcgcctga acggtgtcga cgccgacaac agcgggaaca gctacgtaac
      121 cggaactcct ccgggcggcg gaatattcgt gcagaaatac aattcggccg ggacagcgca
      181 attcggaacc ggttattcga tcgtggaagc gaacgcagat gcgaaagaga tccacgtcag
      241 cgggggcgat atttatctcg gcggaacatt ttacaacacg gcgaatttcg gtttcgatta
      301 cgacgtgatc agtcttgccg gggaaacaag cagttccgtg ttctgggcga aataccaggt
      361 tgacaatacc gcgccggatg tcacactcac cactcctgtt tcgagcccga caaaccagga
      421 cccggttccc gtcacgatca ctttctcgga accggtcaac ggcttcaacc cgaatcccgg
      481 tgatcccaac aacgccacgc tgggaaatgt cgtgcaggtc aatgcgacga cgttcaccgc
      541 ggatcttatt ccttccgttt ccagcgggca gatcgattac acagtcatta acgggctgtg
      601 caccgacaac cacaacaact acaacaattc atccaacacg ctcatcatcg attacgacgc
      661 agtggcgccc acggtttccg tcagcagcac agcgcccgat ccgacgaata tgtcgccgat
      721 tcctgttacg ttcacgtttt cggaaaacgt cagcgggttt actgccggtg atattgttgt
      781 tgcgaacggg acggcgtcga atttcatttc tgtgagcggg agcatttaca ccgcggacat
      841 cacgcctgca tcgggcggaa atgtgagcgt gcaggtgaac ggaaatgcag cacaggacgg
      901 tgcgtcgaac ggaaatacgg catcgaatat cttcagcatc gtttacgatg atgttcatcc
      961 ggccgtcgtc atcatgagta cagcgcccga cccgacgagc acgtcgccta tcccggttac
     1021 gttcacgttt tcggaaaatg tcagcgggtt tactgttggc gatatcgccg tcacgaacgg
     1081 aacggcatcg aattttgttg ccgtgagcgg gaccgtgtac accgcggaca tacttccttc
     1141 tgcgcaggga cttgtttcag tcgatgtgcc cgccgccgta tgtgctgacg gggcgacaaa
     1201 cccgaatgac gcggcaacac agttttcgat cacctaccag ctcacaaccg ggattagcgg
     1261 cccggcagga attcaaccct tcaccgtttt tcccaatccc tgcaccggtt ccttcactgt
     1321 ccgtcttccc gggggatgga cagcaggaac cgtcagcgtg tatgacgtaa cgggcaggat
     1381 gtgtttttcc gcgggaacgg tcaccggcga tcacgttacg ctgcaaaccg gccttcctgc
     1441 gggatgttat tccgtggaga tcgtatcacc gcaaagcaga acgattttac ctctcatcat
     1501 cgatcaacat taaaaacaat cttatgaaaa gaaaactcct tgttttttct tcggctgcgg
     1561 ccctgtgttt ttttcttttt tcctgcggag gaaaaaaaga gaacgccccg gcgaatgatc
     1621 cgggtccatc ttcgcagacc agcacagccc cggctgccaa tgtgcgtccc gactggctgt
     1681 cgtcctcttc cgggaaactg tggaccccgg ataagggtga tgccccgttt tccaaaatga
     1741 ctttttattc tgacggaaca ttgcaggatg ctgtttcgaa aagcaaatgg aaaataaacg
     1801 gaaaccagct gatcgtgaat atcggcttcg acatcagtta cacgatcgca aaagtgtctg
     1861 acaattcgtt cagcctggag acaggcaaag ggaagacatt cgtgtataca gctgcttcgg
     1921 cggatgcagg ttctccgaat gacgcgcagc ccgttccatc ttcttccgca ccggcagatg
     1981 cggctccttc attttcaaaa ggagacaagg tgcaggtgaa ctggaaagga tcctggttcc
     2041 ctgcgacgat cctcgaagtg aaagcggacg ggaaataccg cattcactat acaggttatg
     2101 acagcagctg ggacgaaacc gtttcgtcat cacgcattaa aaaataaacc cgggaaatag
     2161 ttaacaacac ttaaacgcat actgaaatga aaaagaacgc cattttattt actgcaactc
     2221 ttgtgcttgc tgctgcattc atctatgcct gcggcggaaa aaaagccgat catggaaaat
     2281 ggaccgacca ggacattgac aacgcgaaaa agaactgcat gttcggaaaa gatcccgcgc
     2341 tcaccgacga ccagcagaaa gcgatctgcg attgctgggt ggataaagtg atggcggcaa
     2401 gtcctgaccc tgcggaacaa agcaagatcc cgatggacga ggtgaacaaa atgacgacgg
     2461 attgccgcgc ttcggcggga gtgaaatgat gaagaatggt tgactgcctg cgcgctcagt
     2521 gactgaccag cggcaggcgg agaatgactt ctgtcccggc cgcgctcccg ttcgcatttt
     2581 tcaggtcgct gataacgaac gaatcttttt catccgcccc gttcatgcgg cagaattgcg
     2641 ccacgcgtat gcgcgtgatg ctggttcctt tcgattcgtg catggaggat gcgttcttca
     2701 ttttttccga agcggaccgc ccggcgccgt tgtcgcggac ggaaaccacg agcgtacgcc
     2761 cttccacgga aaagcgcagc acgatctcac ccgggccggt gagcggaacc acgccatgcc
     2821 gcacggcatt ttcgaccagc ggctggatga tcataggagg aatgaaagtg tcgtcgggct
     2881 gtatgccgcc cgctgtttcc acgcggaatg agaatttatt ttcaaaacgg aactgctcga
     2941 acagcacata tttttcgagg aaggcgatct cttcctgcag cgtgatcacg gatttgtcgg
     3001 aattgtaaag cgtctgccgc atgagcgagg aaaaatcagc aatggcggcc agtgcttttt
     3061 ctttctcatt cgtgaggatg aaccgttgca gggaattcac ggcgttgaaa acgaaatgcg
     3121 ggttcatctg cgtgcggaaa cgcgcgatct ccgccgcctg ccggttcagc tcttccgtct
     3181 tgttctcgat ctccgctttc tgcatgcgca gctccgaatt tttcctgcgc gtattgaaaa
     3241 acgcaaatac ggaaatgacg aggaggatga agccgagcac ggaaacgccg atgatgaaat
     3301 tcagcttttg cgcttcggct tccctggcct ggctgagcag ctttatcttc tgctcgcggc
     3361 tttgcacgtc gtacttcgcc tgcatggtcg ccatcttttc actggcatcc accgattcga
     3421 tcgaatcatt gatgtcgttg tatttgcgca ggtaatccat cgcttcccgg tacatgcccg
     3481 cgtcgtaata cgcgtccgaa agattgagat aagcgtacca ctgctgtgta agatcgttgt
     3541 gctgctcggc gtatgcgcgc gcggagtcga ggtaaacgat cccttccttc acacgcccga
     3601 ggtgtttcag gctgttgccg taacggttgt aatactgcag tagttcctgc ggatcgttgt
     3661 tcaggcgcgg aaagcgccgg cagatatcga aatagcggat cgcttctccg taatttttcg
     3721 tgtcgtaata atacagcccg taattggtgt tcgtgaggta aagcagatcg ctgtcgcccg
     3781 tgagcttcgt gtatttttcg agctcgtgca gatgcacgag ggaagaatca ttcttccccg
     3841 ccacgtcgta catcgtgaaa atatagctgt ggcagtaagc catctctttg taatctttat
     3901 tctgaaaagc gatggaaagc gcttcgtagt aatactgcgc cgcctgctcg ttcctcccca
     3961 gctcgcggta gcaccagccg agatcgcgga gcgcaagcag ctcctgcttg acgagccggt
     4021 tgctccgcgc tacattgagc gcattgaaat actgcgtcgc ggattcatcg atctgtccct
     4081 ggtaataaat gaaatcgccg aacgcgaaat acgttttgca gacgagcgcg ggatcttttt
     4141 catcgatcgc ttcgctgacg gctttcgtga agcagctcct cgccgaatcg gtactgccgt
     4201 ttttggaaaa ttctttcgcc cggtcgagca tcttcgacgc ctgtccccaa aggcatgcgt
     4261 tcaccgaaag aaggaaaaaa cagagcagtg ggacgttccg cctcataaaa cgaaagtaag
     4321 aaaaggacgg gcaggcatgc ggttgtgttt cgccgtttcc ttccgtgcac gcatatcgct
     4381 taaatttacg ccggcatgtc cacacccgga aaactttccg tcgtcatcat taccttcaac
     4441 gaagaaaaga acatcggccg ttgtctcgat tccgtgaaag acatcgccga cgacatcgtg
     4501 gtgctcgatt ccttttcttc cgacaacaca aaaaagatct gcgaagaaaa aggagcacgt
     4561 tttttccagc atgctttcga cgggcatata gagcagaaga accgcgcgat cacgtttgcc
     4621 gcttacccgt tcgtgctttc gctcgatgcg gacgaagcgc cggacgaaga actgaaacgc
     4681 gccatcagtg aagtgaaaaa aaataaaaca gccgacggct attccatgaa ccggctgaca
     4741 aactattgcg gaaaatggat ccgccattgc ggctggtacc ccgatacaaa gctccgcctg
     4801 tgggacagca cgaagggaaa atggggaggc gtaaatccgc acgacaaata cgagatgaca
     4861 gccgcgggcg cgacggtgaa gcaccttccc gggaatatcc tgcactacag ctattattcc
     4921 gtcgatgagc accggcagca ggctgaaaaa ttttccacca tcgccgcgaa agcgttgggg
     4981 cagagcggaa aatcttccgg cattttcctg gctctcgtga aaacggcggc caagttcatc
     5041 cgtaattatt tcatcaagct cgggttcctc gacgggaaat acggcttcat catctgccgc
     5101 atcagcgcat gggaaacatg gatgaagtac gacaagctca ggcgcatgaa aaaagagaac
     5161 gcgtgaacag gctgtttgaa aaatatccgt tcctctgcag caaaaaattt ctcgctgcgc
     5221 tctacgcggt ggtcgtgacg gccgccacgc tgcaccgcct gtggctcggc gaatcatcgc
     5281 tgaacaactt caccattttc cgctggtcgt tcttcaacct tgcgcacggg aacgacctgt
     5341 atgcgtatca cccggaccag tacaacgacc tgttcaaata cagcccgacg ttcgcgttct
     5401 tcatggcgcc gttctggtgg cttccgaaag cagccgggct tttcgcctgg aatttcatca
     5461 atgcgttcgt tccttttttc gccgtgcagc gattgaaaat aacggacagg gccaaagcat
     5521 tcattcttct tttcgcagga attgaattgc tcggctccat ccagaactgc cagagcaacg
     5581 gcctgatggc gggcctcatc atttttgctt acgccatgat ggaagagcag aagatgtggc
     5641 agtcggcgct gttcctctgt ctcggttttt atgtcaagat attcgcggtc gttgcgggaa
     5701 tattttttct tttcagcgaa cggaaaatgc aattcctcgc atggggcatt ctcatcttcg
     5761 ccgcgctcgg tcttctgccg gcttccgttt gcggtttcgg cggacttatc acgcaataca
     5821 aaagctggtt ccatcttatt gcgaacgatc ctgcccacga gctcaattat tctttcatga
     5881 cgctgacgca acgctggttt tcgttcaccg cgccggacag cgtttacctt gttccgggaa
     5941 tgatcctgct gctgctgccg cttgtgcgga aaaaaatgtg gaaggaagat aatttccgcc
     6001 tgctgctgct tgcgtccgtg ctgctgtggg tggtgatctt caaccacaag gcggaatcac
     6061 cgacttacgt gatcgcggtt ttcggcgcgg gaattttcgg tgcggcggaa aagccgtctg
     6121 ttccgcggac gttcctgctc tggtttgttt tcattctcac ggttcttgcc gcaacggatc
     6181 tcatgcctcc ctttgtccgc gacgagataa tcaaaccgta ttgcctgaaa gcattgccct
     6241 gcatcgtgct gtggctggtc gtgcaatgga aattgctgcg gcaggatttt tccgctgcct
     6301 gaaaattcta cgctccctgt ttttcctccg gcagttcctt catttccttt tgacggacgg
     6361 cacttcccca tggcatcgcg aaaaggaaca gcgaattgaa gaacgcgaag aagatcacgc
     6421 cctgcgaagt ttcgagcgtg tcctcgtcaa tgaacgaaag gaaaagcacg atgaagaagg
     6481 caaggaacag gaagcttccc cagcgtttca tggaagaagc aggatagaaa agcatcagca
     6541 ggaaaaagat aagtcccggt atgccgagtg tcacgagcga ggagaggaac tggttgtgcg
     6601 aatgcatcca gcgccagtct tcgtcgagca tcgtgccgtt gatctggtag cagttcgcaa
     6661 acgctttttc ctgcccgccg gttcctacgc caaacagcag gtgctcgcgc gagcacgtca
     6721 gtgacgtttt gaaaagctct atgcgcatgg tgaccgaatt cccgctcggg ttcgcgccgt
     6781 gcaggtagtg gtatatttcc cacatcacct ggtagatcct gcggccgaac ggggaacgcg
     6841 tggaatcgag cgcatttgtt ttcccgttct cgatggcgtg gatctccgca tcggtaagct
     6901 tgctcatcgc atccgcatct tttttccatc ctttcgaaga aagataacgg atgagcgtga
     6961 tcgaaatagg atttcccttt ttgtcctttc cgccgttcag cacgatgcgg cttcttgcat
     7021 tccagcagga atcgagctcg aaccagcaga tgttgatcat cacctggttg ccgttttcca
     7081 gcatcgggta attcacctgg tgcgtatacg ggtgg
//
LOCUS       DASTSF010000056         7816 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_184791,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000056 DASTSF010000000
VERSION     DASTSF010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 7816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7816
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_184791"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="VFU15_01715"
     CDS             complement(<1..204)
                     /locus_tag="VFU15_01715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716513.1"
                     /translation="MENVTCKVSGKQVAQADARKGEEIQDQLFELIREDHPDFTKDSF
                     ISIAQLTLYRKKYLASLVTAEFGD"
     gene            complement(246..3137)
                     /locus_tag="VFU15_01720"
     CDS             complement(246..3137)
                     /locus_tag="VFU15_01720"
                     /inference="COORDINATES: protein motif:HMM:NF026255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU4716514.1"
                     /translation="MAYFDIVKKIKKSGLLLFAGIFLFTSSLLAQRSTLSGTVTDSTG
                     HNRIFGAMLLLTRVQDSTIAGGGSTDSTGHFVITNVAPGRYSLKCQYTGYKPNTRMIN
                     MPDSSIDLGRIRLTASGHMLKEIEVIGGAQVRVAQNGDTTTYNANGFKTNPDANAEDL
                     VNKMPGVTNDGTGIKVHGEDVKQVLVNGKPYFGDDPNAAMKNIPADMVDQVQIYDKAS
                     DNSQFTGFDDGQSKKTINIITKSQNGTFGKVYAGYGTNDRYNAGAALNFLHGDRRLTL
                     LGMSNNINQQNFSLSDISGAVGSTRGSGRGGYVRPGSAAAGFMVGQQDGISTTNSFGL
                     NYSDKWGSKMQVSGSYFFNVTDNSNNSDLTRNYYTGSDSIFQYRETNTAATRNINHRF
                     NFRFEYTIDSMNALIIAPRLTYQYTDYAKNIDGSNLDALLSSISSTMSNVSSLNTAWN
                     AGGSVTYRHRFAKAGRTLTTSLNTTWNNRLGTGTNYSQSYYSAIDSSGIDQHSSLFSN
                     GYTLSPSLSWTEPAGKKGQMLFSYSPSFSTGMQDKSTNNFDVASADYILLDSSLSNKY
                     AYTYNTQRGGVTYKWAGEKLMIAFGADYQNAMLSGSQSFPPAGSISRSFNSILPDGQL
                     NYKFSKGTNLRIHYRTSNDAPSVSQLQEVVDNSNPLQLKTGNSILRQDYQHMLIAHYG
                     KTNFDKATGFFVFFYGVLTNNYIANSTYIAEADTTLPGGILLKRGSQLSKPINLNGYK
                     NARLFFTYSLPLTKIKSNLGLTLSGGFTSTPSKINQQLNISNTYNVGPGLTLSSNISE
                     KVDFTIAYNGSYNIVNNSYSTQSDNNYFSHTASARFNWMFYKGFVFNTTLDQTFYYGI
                     GNYSTNFLLWNASLGYKFLKDKSLDVRVNAFDILKQNTSVARTVTDTYIEDSRSNTLT
                     QYFMLTVTWNIRHMDAPKKDGQDNSQDQQNQNGGGGNGQHQHQHQGGQK"
     gene            complement(3220..3735)
                     /locus_tag="VFU15_01725"
     CDS             complement(3220..3735)
                     /locus_tag="VFU15_01725"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEU4716515.1"
                     /translation="MKYFLLFLFVFPAAVTGAQETEDSVIIWQKNHYQDTLAPYFSLV
                     DVNGNVWNSDSLRGKTVVLNFWSIYCPACFTELPSINKVPAQFDADSVIFISVLFEKG
                     PKVDSVIAKNHFSYHLCVDGRQIMGDFYNNCFPTHIIIDRNGMIRYNICGMIDEQVLE
                     DALRKTTAVTK"
     gene            complement(3740..4393)
                     /locus_tag="VFU15_01730"
     CDS             complement(3740..4393)
                     /locus_tag="VFU15_01730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716516.1"
                     /translation="MKKTLTLLFSCLSVAAFSQSYDKGAKVIETRFDIGTFHTQSHDK
                     VNNTNSTGAAASKIISASFDYGVLPWLGAGIKFQYDNYFTSKDTITHTPPQADEIVKP
                     SVHAFDPAVFADVHFLRRDHADLLIGGTIGPSFFSYHENDASDTRANGVGLWIDFHFT
                     SRFYFGDHFGINLSAGYNSFNYPDMKAQSSTVPSIDSFSLKGSGGYFGLGLQYRFGD"
     gene            complement(4398..4763)
                     /locus_tag="VFU15_01735"
     CDS             complement(4398..4763)
                     /locus_tag="VFU15_01735"
                     /inference="COORDINATES: protein motif:HMM:NF021221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HEU4716517.1"
                     /translation="MQEAEIIPDNDDMERGKLSAVLSYCTLVGWIVGYVLHQGNKTAY
                     AAFHLRQSIGLAVSALAIMVAFWMTFVFLPFGWMAMRIVNIAYLVFMIIGIINAASGK
                     MKKLPLAGDFFDKIFSSIN"
     gene            complement(4825..7785)
                     /locus_tag="VFU15_01740"
     CDS             complement(4825..7785)
                     /locus_tag="VFU15_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEU4716518.1"
                     /translation="MNRCKLLFAFLLVFQFSFAQKTASGSPDPKPTPAADRLAGLEKR
                     KALADNSIVSNLQFRNVGPAVMSGRVVDIDVNPADPTIFYVAYASGGVWFTDNNGTTF
                     TPVFDNNMLIDIGDIAVNWKDPAKPEIWVGTGECNSSRSSYAGTGIFKSTDGGKTWLN
                     KGLPESHHIGKILIDPTDPKTVYVASIGHLYSPNKERGVFKTTDGGETWKQVLFIDDN
                     TGVIDLFMDPSDPRVIYANAWHRERRAWNFVEGGSKSGIYKTTDGGENWSLITTAASG
                     FPQGEGVGRTGLSIFPGNTNIIYAVVDNQAQDTSVKKDDKLQPSDFRSMTKQQFLSLD
                     RKKLEDYLKRNDFPEKYSADYVTMQVRNGKILPVALADYVSDANSDLFNKPIIGAEVY
                     LSEDGGKTWKKTNTRPLDMMYFTYGYYFGKIWVSPFDKNEVFIGGVTLQKSTDGGKTF
                     AVTEGENQHGDHHALWLDAFRRGHLINGNDGGVNISWDDGKNWFKANTPAVGQFYSVN
                     VDMAKPYNVYGGLQDNGVWSGPSTYQASMEWYSEGRYPYERLLGGDGMQVQVDTRDNN
                     TIYTGFQFGHYFRVDKTTHKETELKINIDLGDQPLRFNWQAPIWLSAHNQDVLYIGAQ
                     KVFRSLDQGDHFEAISGDLTLGAKTGDVPYGTISTIHESPVKFGLIYAGSDDGLIHVT
                     KDGGSTWTRISDKLPQHMRVNRVIASSHVEGRVYAVLSGFQWDHFNPYVFVSEDYGAN
                     WKQIGTDLPMEPVNVIREDPENENLLFVGTDNGLYVSVNRGSSFMLMNNGIPPVAVHD
                     LVIQPREHDLVVGTHGRSIYIGNISALEQLTDSTLKQDLVLFDPGPIKTHYDYIDTDH
                     EPKLITTNTLDLAMFSNLSGPCTISLLSADGNHTFSMYKDTLEKGLNYFHRDITVDSL
                     YGDSYQSWLDDIGEKEKPVQLPNGSYVLKPGDYIIEVNCGSKTKSVKINIRKPKSREN
                     IPAPSPGEMEWRKD"
BASE COUNT         1695 a         1975 c         2137 g         2009 t
ORIGIN      
        1 atcgccgaat tcagccgtta ccagcgaggc aaggtatttt ttccggtaga gagtgagctg
       61 cgcgatcgag atgaacgaat ccttcgtgaa atccgggtga tcttccctga tcagctcgaa
      121 aagctggtcc tggatctctt cacctttccg tgcatccgcc tgcgcaacct gttttccgga
      181 aactttacac gttacgtttt ccattggaac aaaaagagga ataaagataa tccgggcagg
      241 gaaatttatt tctgtccgcc ctggtgctga tgctggtgct ggccgttacc accgccgcca
      301 ttctggttct gctggtcctg gctgttgtcc tgtccatcct ttttaggagc atccatgtga
      361 cggatgttcc aggtgacggt cagcatgaaa tactgcgtca gcgtgttgga gcggctgtcc
      421 tcgatgtacg tatcggtaac cgtcctggca acgctggtgt tctgcttcag gatgtcgaac
      481 gcgttgactc tcacgtcaag cgatttgtcc ttgagaaatt tatacccgag cgatgcgttc
      541 cacagcagga agttcgtgct gtaattcccg atgccgtaat agaaggtctg gtcgagcgtg
      601 gtattgaaca cgaatccttt atagaacatc cagttgaacc gtgcggaagc ggtgtgcgaa
      661 aaataattat tgtcggactg cgtggagtag gaattgttca cgatgttgta gctgccgtta
      721 taggcgatcg tgaaatcgac tttctcgctg atgttgctgc tgagcgtaag gccgggaccg
      781 acattatacg tgttcgaaat gttgagctgc tggttgatct tcgaaggagt ggatgtgaat
      841 ccgcccgaaa gtgtgagtcc gaggttgctt ttgatcttcg tcagcggcag gctgtaggtg
      901 aaaaacagcc gcgcgttctt gtaaccgttc aggttgattg gcttcgagag ctggcttccg
      961 cgtttcagca ggatcccgcc gggaagtgtt gtgtccgctt cggcgatgta agtggaattg
     1021 gcaatgtaat tgttcgtaag aacaccatag aaaaagacaa agaacccggt cgctttgtcg
     1081 aaattggttt tcccgtaatg cgcgatgagc atgtgctggt aatcctggcg aagaatggaa
     1141 ttcccggttt tcagctggag cgggtttgaa ttgtccacca cttcctgcaa ctgcgaaacc
     1201 gaaggcgcgt cattcgacgt gcggtaatga atgcgcaggt tggttccctt ggaaaatttg
     1261 taattgagct gcccgtcggg aagaatgctg ttgaaagagc ggctgatgga accggcgggc
     1321 gggaacgact gtgaaccgga aagcatggca ttctggtaat cggcgccgaa agcgatcatc
     1381 agcttttcac ccgcccattt gtacgtcacg ccgccacgct gggtgttgta ggtatacgcg
     1441 tatttattcg acaggctcga gtcaagcagg atgtaatcgg ccgatgcaac atcgaaatta
     1501 ttcgtgctct tatcctgcat ccccgtggaa aaagaaggcg aatagctgaa cagcatctgt
     1561 cctttttttc cggccggctc cgtccaggaa agagaagggg aaagcgtata cccgttcgag
     1621 aacagggaag agtgctgatc gatgccgctg ctgtcgatcg cggaatagta gctctgcgaa
     1681 taatttgtgc cggtcccgag acggttgttc caggttgtgt tcaggcttgt tgtaagtgtt
     1741 cttcccgctt tcgcgaaacg gtgacggtag gtgacggatc cgcccgcgtt ccacgctgta
     1801 ttgagcgatg aaacattgct cattgtggaa gagatggagg aaagcaaagc gtcgaggttg
     1861 ctgccgtcga tattcttcgc gtaatcggta tactggtagg taagccgcgg tgcgatgatc
     1921 agcgcattca tcgaatcgat cgtgtattcg aaacggaaat tgaaccggtg attgatattg
     1981 cgcgtggccg ccgtgttggt ttccctgtat tggaaaatgc tgtcgctccc ggtgtaatag
     2041 ttccgtgtca ggtcggaatt gttgctgttg tccgtcacgt tgaagaaata gcttccgctg
     2101 acctgcattt tgcttcccca cttgtcgctg taattcagcc cgaacgaatt ggtggtggaa
     2161 attccatcct gctggccgac cataaaccct gcggctgcgc ttccgggacg cacataaccg
     2221 cctcgcccgc ttcccctcgt tgatccgacc gcgccggaaa tatccgacag ggaaaaattc
     2281 tgctggttga tgttgttcga catgccgagc agcgtcagcc gccggtcacc gtgcagaaag
     2341 ttcagcgccg ctcctgcgtt gtaacggtca tttgttccat agcccgcgta cacttttcca
     2401 aatgttccgt tctggctttt cgtgatgatg ttgatcgttt ttttcgactg gccgtcatcg
     2461 aagccggtga actgcgagtt gtcgctcgct ttgtcgtaga tctgcacctg gtcgaccatg
     2521 tctgccggga tgttcttcat cgccgcattc ggatcgtcgc cgaaatacgg ttttccgttc
     2581 accagcacct gcttcacatc ttcaccgtgc actttgatgc ccgtgccgtc attggtaacg
     2641 cccggcattt tattcacgag gtcttccgca ttcgcatcgg gattggtctt gaacccgttc
     2701 gcattgtacg tggtcgtgtc gccgttctgc gccacacgga cctgggctcc gccaatgact
     2761 tcgatctcct tcagcatgtg cccgcttgct gtgagacgga tccgcccgag atcgatgctc
     2821 gaatccggca tgttgatcat gcgtgtattc ggtttgtatc ctgtatactg gcatttcagg
     2881 gaataacgac ccggcgcgac attggtgatg acgaaatgcc cggtggaatc agtgcttccg
     2941 ccgcctgcaa tggttgaatc ctggacgcgc gtcagcagga gcatggcccc gaaaatgcgg
     3001 ttatgcccgg tgctgtcggt aacggtgcct gaaagtgtgc tgcgctgggc gagcaacgac
     3061 gacgtgaaaa ggaatattcc ggcgaatagg agaagccctg atttttttat ttttttcaca
     3121 atatcgaagt aggccataaa agtagtgaga ttggttaagc gtttgacgac aacgctgaac
     3181 gcaaccgttc ttaaaaattg caaataggat gaacgggaat tatttcgtga cggcggttgt
     3241 ttttcggagg gcatcctcca gcacctgttc gtcgatcatc ccgcagatgt tataacggat
     3301 catcccgttc cggtcgataa tgatgtgcgt cgggaaacaa ttgttgtaaa aatcacccat
     3361 gatctgtctc ccgtccacgc acaggtgata ggaaaagtga ttcttcgcga tcacggaatc
     3421 gactttgggt cctttctcaa acaggacgga aataaaaata acgctgtccg catcgaactg
     3481 tgcgggaact ttattgatcg acggaagttc ggtaaaacat gccgggcaat agatcgacca
     3541 gaaattaagc acgacggtct ttccgcgcag cgaatcgctg ttccatacat tcccgttcac
     3601 atcgacgaga ctgaaatacg gcgcgagcgt atcctggtaa tggttcttct gccagatgat
     3661 gacagaatct tccgtttcct gcgcgccggt caccgcagcg ggaaacacaa acaggaaaag
     3721 caggaaatat ttcatgcgct cagtcaccga aacggtactg cagtcccagg ccgaaataac
     3781 cgccggatcc tttcagggaa aacgaatcga tggagggaac ggtgctgctt tgcgctttca
     3841 tgtcgggata attgaagctg ttgtagccgg cgctgaggtt gatcccgaaa tgatcgccga
     3901 aataaaaacg cgaagtgaaa tgaaaatcga tccacaggcc gacgccgttg gcgcgtgtat
     3961 ccgatgcatc gttctcgtgg tagctgaaaa aagaaggccc gatggtcccg ccgatcagca
     4021 ggtcggcatg gtcccgccgc aggaaatgca cgtcggcaaa cacggcagga tcgaacgcat
     4081 gcacgctggg tttgacgatc tcgtctgcct gcggcggcgt gtgcgttatc gtgtccttgc
     4141 tcgtaaaata attatcatac tggaatttga tccccgcgcc cagccagggc agaacgccgt
     4201 aatcgaaaga agcgctgatg atcttgctcg ccgccgcgcc ggtgctgttg gtattgttca
     4261 ctttgtcatg cgactgggtg tggaatgtgc cgatgtcaaa gcgggtttcg atcacttttg
     4321 cgcctttgtc gtaagactgc gagaacgccg caacagacag gcaggaaaag agcagggtca
     4381 gtgttttctt catgtggtca gttgatgctg ctgaatattt tatcgaaaaa atcaccggca
     4441 agcgggagtt ttttcatttt tccactggct gcatttatta tgccaatgat cataaaaaca
     4501 agatacgcaa tattgacgat ccgcatcgcc atccatccga acggaagaaa aacgaacgtc
     4561 atccagaacg caaccatgat cgccagcgcg gaaacggcca gcccgatcga ctgccggaga
     4621 tgaaatgcgg cataggccgt tttgtttccc tggtgcagca catagcccac gatccagccg
     4681 accagcgtac agtaggagag gacagcggat aattttccgc gttccatatc gtcgttgtcg
     4741 gggatgatct ctgcttcctg cataaattaa aattaagcga tttatttttt ccggagaatg
     4801 cgcaaggcag gccccgctta atttttaatc ctttctccat tccatttctc ccggcgaagg
     4861 cgccggaata ttttcccggc ttttcggttt gcggatattt attttcactg acttcgtttt
     4921 cgaaccgcaa ttcacttcga tgatgtagtc gccgggtttc agcacgtagc tgccgttcgg
     4981 aagctgcacc ggtttttcct tttccccgat gtcgtcgagc cacgactggt agctgtcgcc
     5041 gtaaagcgag tcgacagtga tgtcgcggtg aaaatagttc agaccttttt cgagcgtgtc
     5101 tttgtacatg ctgaatgtat ggttcccgtc ggcggaaagc aggctgatcg tgcacggacc
     5161 gctgaggttc gagaacatcg cgaggtcgag cgtgttcgtc gtgatgagct tcggttcgtg
     5221 atcggtgtcg atgtaatcgt aatgcgtttt gatgggaccc ggatcgaaga ggacgagatc
     5281 ctgcttcagg gtgctgtcgg tgagctgctc gagcgcagaa atgttcccga tgtagatgga
     5341 acgtccgtgc gtgccgacaa caagatcatg ctcgcgcggc tggataacga gatcatgaac
     5401 agccaccggc ggaattccat tgttcatgag catgaacgaa cttccgcggt tgacggaaac
     5461 atacaacccg ttatccgtac cgacgaaaag caggttttca ttttcaggat cttcccggat
     5521 cacgttcacc ggttccatgg gaagatcagt tccgatctgt ttccagtttg ctccataatc
     5581 ttcggaaaca aaaacgtacg ggttgaaatg gtcccactgg aaaccgctga ggacagcata
     5641 tacgcgccct tccacgtgcg acgacgcgat cacccggttg acgcgcatgt gctggggcag
     5701 cttgtctgaa attcttgtcc atgtgctgcc tccgtctttc gtgacgtgaa tgagcccgtc
     5761 gtcgctcccg gcgtaaatga gcccgaactt caccggcgat tcatgaatgg tggagatcgt
     5821 tccgtacggc acgtcgccgg tttttgcacc gagcgtaagg tcgccgctga tcgcttcgaa
     5881 atgatcgccc tggtcgagcg aacgaaatac tttctgcgcg ccgatgtaga gtacgtcctg
     5941 gttatgcgcc gaaagccaga tcggcgcctg ccagttgaac cggagcggct ggtcgccgag
     6001 atcgatgttt attttcagtt ccgtctcctt gtgcgtcgtc ttgtccacgc ggaaataatg
     6061 accgaactgg aaaccggtgt agatcgtgtt gttgtcgcgc gtatctacct gcacctgcat
     6121 gccgtcgccg ccgagcaggc gttcgtacgg gtagcggcct tcgctgtacc attccatgct
     6181 ggcctggtag gtgctcggtc cgctccacac gccgttgtcc tgcaatccgc cgtaaacgtt
     6241 gtacggcttc gccatatcga cattgacgga atagaattgt cccaccgccg gtgtgttcgc
     6301 cttgaaccag ttctttccgt cgtcccatga aatgttcacg cccccgtcgt tgccgttgat
     6361 gagatgtccc ctgcgaaacg cgtcgagcca gagcgcatgg tgatcgccgt gctggttttc
     6421 tccctcggta accgcgaatg tttttccgcc gtccgtgctt ttctgcagtg tcacgccacc
     6481 gatgaaaact tcatttttat cgaacggcga aacccatatt tttccgaaat agtaaccgta
     6541 ggtgaaatac atcatgtcga gcggcctggt gttcgtcttc ttccaggttt ttcccccgtc
     6601 ttcggaaaga taaacttccg caccgatgat aggtttgttg aagagatcgc tgttggcgtc
     6661 ggaaacataa tcggcaagcg caaccggaag tattttcccg ttacggacct gcatcgtgac
     6721 gtaatccgcc gaatattttt ccgggaaatc attccgcttc aggtaatctt ccagcttttt
     6781 ccggtcgagg ctgaggaact gctgcttcgt catcgaacgg aaatcggaag gctgcagctt
     6841 atcgtctttt ttcacggacg tatcttgcgc ctggttatcg acgaccgcgt aaataatgtt
     6901 cgtattgccg ggaaaaatgc tcagtcccgt ccggcccacg ccttcgccct gcggaaatcc
     6961 gcttgctgct gtggtgatca gcgaccagtt ttcgccgccg tcggtggttt tataaatgcc
     7021 ggatttgctt ccgccctcca cgaaattcca tgcacggcgt tcgcggtgcc aggcgttcgc
     7081 gtaaatgact cgcggatcgg acggatccat gaacagatcg atcacgccgg tattgtcgtc
     7141 aatgaacaaa acctgtttcc atgtttctcc tccgtcggtg gtcttgaaaa cgccgcgctc
     7201 tttattcggg gaatacaaat gaccgatgct cgcgacatag accgttttcg gatcggtggg
     7261 gtcgataagg attttcccga tgtgatgcga ttcgggaagg cctttgttca gccatgtttt
     7321 tccgccgtcg gtgcttttga aaattcctgt tcccgcatac gaagaacggc tcgaattgca
     7381 ttccccggtt cccacccaga tctcgggctt tgccgggtct ttccagttca ccgcgatgtc
     7441 gcctatgtca atgagcatgt tgttgtcgaa cacaggtgtg aacgtcgttc cgttgttgtc
     7501 cgtaaaccac acgccgccgg aagcataggc cacgtaaaag atcgtcgggt cggccgggtt
     7561 gacatcaata tcgaccacgc gtccgctcat cacggccgga cctacgttgc ggaactgcag
     7621 gttggaaaca atggaattat ccgcgagcgc tttccgtttt tcaagtccgg cgagacgatc
     7681 ggcggccggt gtaggtttcg gatccgggga accggaagct gttttttgcg caaatgaaaa
     7741 ttgaaaaacg agcaggaaag cgaaaagaag tttgcagcgg ttcatgttgt gtggattgta
     7801 aagaagggct gaagtt
//
LOCUS       DASTSF010000057         7878 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1747221,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000057 DASTSF010000000
VERSION     DASTSF010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 7878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7878
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1747221"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="VFU15_01745"
     CDS             complement(<1..266)
                     /locus_tag="VFU15_01745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716519.1"
                     /translation="MLLILLLSFLGFFGVLFTWVLLAPVRLDIDTTKNLYSFRVIPFF
                     RLQWMTEPVPGHAEMYIFGVRKKLSVHTQEERKTVKPEKKAKRRS"
     gene            complement(375..1082)
                     /locus_tag="VFU15_01750"
     CDS             complement(375..1082)
                     /locus_tag="VFU15_01750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716520.1"
                     /translation="MKNVFFPLFILFGVIASGFVVVKTDKNEKKFTEDLIPDSAVFSS
                     TGRNTYFILEPGYRLVLKGMDGKDSSTLIITVLNETKKVGGIETRVVEENESVNGRTI
                     EISRNFFACCKNNNSIYYFGEETDLFDKDGNKIPGKDSWLATGNNKPGLQMPGLVLLG
                     ARYYQEIAPGIAMDRAEILSTDNAFTTPSGTFKNVLKTEETTPLEPLEKEYKLYAPGI
                     GLIKDEGLVLVQYGFVN"
     gene            1414..3315
                     /gene="htpG"
                     /locus_tag="VFU15_01755"
     CDS             1414..3315
                     /gene="htpG"
                     /locus_tag="VFU15_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010520545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HEU4716521.1"
                     /translation="MSTGTINVQTENIFPIIKKFLYSDHEIFLRELVSNAVDASKKLK
                     ALSSLGEYSGELGTLAVTVSVDKENKTITVSDNGIGMTEEEVEKYITQVAFSGAEEFV
                     QKYKDKTDAVQGIIGHFGLGFYSAFMVAAKVELVTKSYKDDASAVKWSCDGSPNYTIE
                     PAEKAGRGTDVILHVAEDSLEFLEDSRIRGILTKYCKFLPVEIRFGEHEEEITPAEKD
                     ADGKETKPAVKETVPTVINNPNPAWTKKPADLTDEDYKKFYRELYPMNFEEPLFWIHL
                     NVDYPFNLTGILYFPKIKKSFEAQREKIQLYCNQVFVTDSVEEIVPDFLTLLRGVIDS
                     PDIPLNVSRSYLLSDSNVKKISSHITKKVADKLEELFKKDRADFEAKWEDIRIFVQYG
                     MISDDKFYDRAKKFALLGNTEKKFFTLDEYREKIAPLQTDKNSKVVFLYTMNAEEQHS
                     FVDAAKARGYDVLVFDSMIDPHYINALESKLENVSFVRVDSDVADRLVKKDDGIPSKL
                     SDKEQEEIKKTVEDVLPKEKFTVTFESLLDTDPPMMVTRPEFMRRMKDMSALSGGSRG
                     MFGELPDMHNLVVNSNHPLVAKILAESDGEKKKAIAKQAADLALLAQGLLKGEELTNF
                     IKRSTEMIG"
     gene            3423..4034
                     /locus_tag="VFU15_01760"
     CDS             3423..4034
                     /locus_tag="VFU15_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007215350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEU4716522.1"
                     /translation="MKRGTFVLLTFVFVILVIFMNGCGAYNNMVNGREEVRKTWQNVE
                     TFYQLRIDKIPNLVATVQGQGDFEKSTLEAVINARASATKVTVDPTKLDAASLQQYEQ
                     AQGALSSALGRLLVVSENYPNLQTNSAFTELRAEISETENRIQVARSDYNNKAADFNA
                     MIQRFPRNIWAGVFGFSPFPYFQADQGADKAPKVNFNFKDSAK"
     gene            4038..4469
                     /locus_tag="VFU15_01765"
     CDS             4038..4469
                     /locus_tag="VFU15_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010526058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HEU4716523.1"
                     /translation="MKDIFSEEQLKQVSAAIEAAEKNTSGELRVHIGRRCAGDPVKCA
                     QRLFTHLGMKKTKRRNGVLFYLAVVSRKFAVIGDEGIHAKVGSDFWHSVREKMENEFR
                     EGRFTEGLISGITEAGRQLEHYFPPEEGDSNELPDEISIEK"
     gene            4466..5251
                     /locus_tag="VFU15_01770"
     CDS             4466..5251
                     /locus_tag="VFU15_01770"
                     /inference="COORDINATES: protein motif:HMM:NF016424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HEU4716524.1"
                     /translation="MKTYFRLFLFSFFLLAQGTVFALPGDDQYPGIPARPDPPRLVVN
                     LSQQFPLFLSETETQQLETKLENFSNETSNQIAIVIVDDLGGLPAWEYATDLGNKWGV
                     GSKKFDNGVVILVKPTGGEGNRDVFIAVGTGLNAKINATRSIHVVDDILKPGLASGEY
                     YDALDKATDELMKMAKGEYHDPAPKRFSSPIAFFIGAVILFIILRLFFGFFLGGGGGM
                     TMGGGRYFGGGFGGGGGFGGGGGGGFGGFGGGGFSGGGSGGKW"
     gene            5264..6412
                     /locus_tag="VFU15_01775"
     CDS             5264..6412
                     /locus_tag="VFU15_01775"
                     /inference="COORDINATES: protein motif:HMM:NF005243.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEU4716525.1"
                     /translation="MKIAISGGGIAGLTAAVALHEKGHDVTVFEAAKELRVSGAGISL
                     ASNAMRALEKIGLGEQLRTYGRFLDRVMICNPEGEVLVENDSRKISGDYNVINYTIHR
                     ADLHAILLRNFPNKKIILGKKAVQTTTHGDKEVLHFSDDTREEADLILACDGIHSPLR
                     LQFVPKSLPRYAGYTCWRGIANYFPGSMDEDAMTETWGARGRVGLVPVSGDRVYWYIC
                     LNTKMENDPIYDAWSILHLRKHFLDYHRPIDDVLKNTRPEAILKNDIFDLKPLKRFAF
                     GKVLLMGDAAHAMTPNLGQGACQAIEDAVLLPLYLQRFPDVKEALREFEKVRVPRTAR
                     IIRTSRRFGQLAQLQHPLFSNLRNFLVRIAPERMNHRQFRFLFDVDFD"
     gene            6489..6866
                     /locus_tag="VFU15_01780"
     CDS             6489..6866
                     /locus_tag="VFU15_01780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716526.1"
                     /translation="MIEEYGRVLSRIMGLTDPSALGKALGEIRESYLTYFGVDAAVID
                     KFHPDDLPLFLTKEHHFTFGQLEALAKGITAEGNLSPDEMVAKMKYRKALALYLWLEK
                     EDTQTFSIHRKQAVNDLMNRIGQ"
     gene            6990..>7878
                     /locus_tag="VFU15_01785"
     CDS             6990..>7878
                     /locus_tag="VFU15_01785"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU4716527.1"
                     /translation="MSRFLRLPALLLLLLPAFVMGQQNAELVNSGQLLFEGNLYYYLG
                     KYGKAEETFKRVGRNDTNYAVITRDLARTYSDDDEDSLCLMTCVKGLDLHSVYDVDFY
                     NYMGISLKEMNRYDSALKVYDKAIGLFPYAHLLYYNKGMVYYKQKNYVEAQKWFQEAV
                     RINPYYSQSHFMLGKSCAEQGRIIPAVLSFEYFLMVDPSSDKAPKVVTTIEDLVTGDY
                     QPDPDQKLDDDESGDKCFADVTDAINSQTALQAGYRNKTGINLKVVKQLQYLFEKLKY
                     QEGTNNWWMDFYVPFYTELQ"
BASE COUNT         1987 a         2040 c         2186 g         1665 t
ORIGIN      
        1 gaacgccgtt tcgctttttt ttccggcttc accgtttttc tttcttcctg cgtatgaacg
       61 gaaagttttt tccgcacgcc gaaaatgtac atttcagcat gacccggaac cggttctgtc
      121 atccactgca aacgaaagaa cgggatgacg cgaaagctgt acaggttctt cgtggtgtcg
      181 atgtcgaggc gaaccggcgc aagcagcacc catgtgaaca ggactccgaa aaaaccgaga
      241 aaggaaagaa gtaaaatgag cagcacgctt cctgtttttt gaacgtctca aaagtagacg
      301 cgcgggcagg gaggaacgat gacgctgcgc acagcgtgcg ctgagaatga tcaaaaggaa
      361 aagcgggcga acgctcagtt cacgaagccg tactgcacca gcacgagtcc ttcgtctttg
      421 atcagcccga tgccgggagc gtacagcttg tattcttttt ccagcggttc gagcggcgtg
      481 gtctcttcgg tcttcagcac atttttaaac gtgcctgacg gcgtcgtgaa tgcattgtcc
      541 gtgctgagga tttccgcgcg gtccatcgcg atgcccggtg cgatctcctg gtaataacgt
      601 gcgccgagca ggacgagccc gggcatctgc aggccgggtt tgttgtttcc tgtggcgagc
      661 caggaatctt tccccggaat tttgttcccg tccttgtcaa acaggtcggt ttcttcgccg
      721 aaataataaa tgctgttgtt gtttttgcag caggcgaaaa aattgcgcga gatctcgatc
      781 gttctcccgt tcacggattc attttcttcc acgacgcgcg tttcgatacc gcccactttt
      841 tttgtttcat tcaggacggt gatgatgagc gtcgaggaat ctttcccgtc cattcctttc
      901 agcacgagac ggtaacccgg ctcgagaatg aaatacgtgt tgcgtcctgt gctggaaaat
      961 acggcgctgt cgggaataag gtcttcggtg aatttctttt cgttcttgtc cgttttgacg
     1021 accacgaagc cggaggcaat gacaccgaag agaatgaaaa gaggaaagaa aacgtttttc
     1081 atggcggaga tgattaggat aaagcaatat tggaaatgat tctgaagaaa aactggatgc
     1141 gggtaaggaa attcaacgat gaaatgaacg tgtaatgccg ggatcaatcg acaccgaagg
     1201 aaaaaccgga aaccggcatg tgtcaatctg tcagtgaaat ctggctggca tacggtttgg
     1261 gaatgtgatg cgcccggcgg gaataagtta gccctggcgg acgcggaaag cccggggctt
     1321 gaagcaaacg acaggagctt ggtaactttg acgcacagct gtcaccgctc caaaacataa
     1381 ttcaacatac aaaatccaac attcagaatc gatatgagca caggaaccat caacgtacaa
     1441 acggaaaaca ttttcccgat catcaagaaa ttcctctatt cggatcacga gatctttctc
     1501 cgcgagctgg tgtcgaatgc ggtggacgct tccaaaaagc tgaaagcgct ttcctcgctc
     1561 ggcgaataca gcggcgagct gggcaccctg gctgtcaccg tgtcggtgga caaggaaaac
     1621 aaaacgatca ccgtttcgga taacggcata gggatgacgg aagaggaagt ggaaaaatac
     1681 attacgcagg tggcgttttc cggtgcggag gagttcgtgc agaaatacaa agacaaaacc
     1741 gacgccgtgc agggcatcat cgggcatttc ggtctgggat tttattcggc gttcatggtg
     1801 gcggcgaaag tggagctggt gacgaaaagc tacaaagacg atgcttccgc cgtgaaatgg
     1861 agctgcgacg gaagtccgaa ctataccata gaacccgcgg aaaaagcggg ccgcggaacg
     1921 gacgtgatcc tgcacgtggc tgaagattcg ctcgagttcc tggaagattc ccgcatccgc
     1981 ggcatactca cgaaatactg caagttcctt cccgtcgaga tccgtttcgg cgagcacgaa
     2041 gaagagatca cgccggcgga aaaagatgcg gacggaaaag aaacaaagcc cgccgtgaag
     2101 gaaaccgttc ccacggtgat caacaacccg aacccggcct ggacgaaaaa gccggccgat
     2161 ctcaccgacg aggattacaa aaaattctac cgcgagctgt acccgatgaa tttcgaggag
     2221 ccgctgttct ggatccacct caacgtcgac tacccgttca accttacggg catcctgtat
     2281 ttcccgaaga tcaaaaaatc attcgaggcg cagcgcgaaa agattcagct ctactgcaac
     2341 caggtgttcg tgacggattc ggtggaagag atcgttcccg attttctcac gctgctccgc
     2401 ggtgtgatcg attcgccgga catcccgctg aacgtttcgc gttcctacct gctgagcgat
     2461 tcgaacgtga agaagatctc ttcgcacatc acgaagaaag tggccgacaa gctcgaagag
     2521 cttttcaaaa aagaccgcgc cgacttcgaa gcgaaatggg aagacatccg cattttcgtg
     2581 cagtacggca tgatcagcga cgacaagttc tacgaccgtg cgaagaagtt cgcgctgctc
     2641 ggcaatacgg aaaagaaatt cttcacgctc gacgaatacc gggagaagat cgcgccgctg
     2701 cagacggaca agaacagcaa agtcgttttc ctctacacga tgaacgctga agagcagcac
     2761 agcttcgtgg atgcggcgaa agcccgcggc tacgatgtgc tcgtgttcga cagcatgatc
     2821 gatccgcatt acatcaacgc gctcgagtcg aaactggaaa atgtgtcctt cgtgcgcgtg
     2881 gacagcgacg tggccgaccg tctcgtgaag aaagacgacg gcattccttc gaagctttcc
     2941 gacaaggagc aggaagagat caaaaagaca gtggaagacg tgctgccgaa ggaaaaattc
     3001 accgttacgt tcgagtcgct gctcgacacc gatccgccga tgatggtgac gcgtcccgaa
     3061 ttcatgcgcc gcatgaagga catgagcgcg ctgtcgggcg gaagccgcgg catgttcggt
     3121 gagctgccgg atatgcacaa cctcgtggtg aattccaatc acccgctcgt ggcgaagatc
     3181 cttgcggaaa gcgacggtga aaagaaaaaa gcgatcgcga agcaggccgc cgacctcgcg
     3241 ctgctcgcgc agggactgct gaaaggagaa gagctcacca attttatcaa acgcagcacg
     3301 gaaatgatcg ggtgaaaagt tgaaggcggg gaagtttcgg gtttcgggtt tgataaaacg
     3361 aaagcaggta accaaaaaca agagaacaac ttccctacct tgcaaaataa taactgaaaa
     3421 acatgaaacg cggaaccttc gtattactca cattcgtctt cgtcatcctt gtcattttca
     3481 tgaatggatg cggagcgtac aacaacatgg tcaacggacg cgaggaggtg cgcaagacat
     3541 ggcagaacgt ggaaacgttc taccagctgc gcatcgacaa gatccccaac ctggttgcca
     3601 ccgtgcaggg ccagggcgat tttgaaaaat caaccctcga ggccgtgatc aacgcacgcg
     3661 ccagcgccac gaaagtgacg gtcgatccca ccaagctcga cgccgcttcg ctgcagcaat
     3721 acgagcaggc gcagggtgcg ctctcgagtg cgctgggaag attgctggtg gtgtcggaaa
     3781 actacccgaa cctgcagacg aattccgcgt tcaccgagct gcgtgcggag atttccgaaa
     3841 cggagaaccg catccaggtg gcgcgcagcg attacaacaa caaggcggcc gacttcaacg
     3901 cgatgatcca gcgtttcccg cgcaacatct gggcgggtgt gttcgggttc tcgccgttcc
     3961 cgtatttcca ggccgaccag ggcgcagaca aggcgccgaa ggtgaatttc aatttcaaag
     4021 acagcgcaaa ataagcggtg aaagatattt tcagcgaaga acagctaaag caggtcagcg
     4081 cagcgatcga agcggccgag aaaaacacct cgggcgagct tcgcgtgcac atcggcaggc
     4141 gctgtgctgg cgacccggtg aaatgcgcgc agcgcctgtt cacgcatctc ggcatgaaaa
     4201 aaacgaagcg gcgcaacggc gttctttttt atctcgccgt cgtttcccgg aagttcgcgg
     4261 tgatcggtga cgaaggcatc cacgcaaaag tgggaagcga tttctggcat tcggtgcgtg
     4321 aaaaaatgga gaatgagttt cgggaaggac gcttcaccga aggattgatc agcggcatca
     4381 ccgaagccgg gcggcagctt gaacactatt tcccgccgga ggaaggcgac agcaatgaat
     4441 taccggatga aatcagcatc gaaaaatgaa aacatatttc cggttatttc tcttttcatt
     4501 tttccttctt gcacaaggaa cggtattcgc attgcccggc gacgatcaat atcccggcat
     4561 tcccgccagg cccgatccgc cgcggctggt ggtgaacctg tcgcagcagt tcccgttgtt
     4621 cctttccgaa acggaaacgc agcagctcga aacgaagctg gaaaatttca gcaacgagac
     4681 ctcgaaccag atcgcgatcg tgatcgtgga cgatctcggc gggctcccgg cctgggaata
     4741 tgccaccgat ctcggcaaca agtggggcgt aggctcgaaa aaattcgaca acggcgtcgt
     4801 tatcctggtc aagccgaccg gcggcgaagg aaaccgtgac gtgttcatcg ccgtgggaac
     4861 cggcctcaac gcgaagatca acgctacgcg ttccattcac gtcgtcgacg acatcctcaa
     4921 gccgggcctt gcttcgggcg agtattacga tgcgctggac aaagccacgg acgagctgat
     4981 gaaaatggcg aaaggcgaat accacgaccc ggctccgaaa agattttcaa gcccgatcgc
     5041 atttttcatc ggtgcggtta tcctgtttat tattctccgc ctcttctttg gctttttcct
     5101 cggcggcggc ggcggaatga caatgggcgg tggacgatat ttcggtggcg gtttcggcgg
     5161 tggcggaggc tttggcggcg gaggcggcgg tggtttcgga ggtttcggcg ggggcggatt
     5221 cagcgggggc ggttccggcg ggaagtggta atattgtatt cccatgaaaa tcgcgatttc
     5281 cgggggaggc attgcgggac ttaccgctgc agttgcactc cacgaaaaag gtcatgacgt
     5341 aacggttttt gaagcggcga aagagctgcg cgtgtccggt gccggaattt cgctggcgtc
     5401 gaacgccatg cgtgcgctgg aaaagatcgg tctcggcgag cagctgcgca catacgggcg
     5461 ttttctcgac cgcgtcatga tctgcaatcc cgaaggagaa gtgctcgtgg aaaacgattc
     5521 ccggaaaatt tccggtgatt acaacgtgat caattacacg atccaccgcg ccgatcttca
     5581 tgcgatcctg ctgcgcaatt ttccgaacaa gaagatcatc ctcgggaaga aggcggtgca
     5641 gacgaccacg cacggcgata aggaagtgct tcacttcagc gacgacacac gcgaggaagc
     5701 ggaccttatc ctcgcctgcg acggaattca ttccccgctg cgcctgcagt tcgttcccaa
     5761 atcgcttccg cgttatgccg gttacacctg ctggagaggc attgccaact atttcccggg
     5821 ttccatggac gaagacgcca tgacggaaac gtggggcgca cgcgggcgcg tcgggctcgt
     5881 gccggtcagc ggcgaccgtg tgtactggta catctgcctc aatacgaaaa tggagaacga
     5941 cccgatctac gacgcgtggt ccatccttca tctccggaaa cattttctcg attatcaccg
     6001 ccccatcgat gacgtgctga aaaatacccg gcccgaagcg atcctgaaaa acgacatttt
     6061 cgacctgaag ccgctgaaac gttttgcttt cggaaaagtg ctgctgatgg gagatgccgc
     6121 gcatgcgatg acgcccaatc tcgggcaggg cgcgtgccag gcgatcgaag atgccgtatt
     6181 gctcccgctt tacctccagc gctttcccga tgtgaaggag gcgctccgtg aatttgaaaa
     6241 agtgcgcgtt ccccgcactg cgcggatcat ccgcacctcg aggcggttcg ggcagctggc
     6301 gcaactgcag cacccgcttt tttccaacct gagaaatttc ctcgtgcgca tcgcgccgga
     6361 gcggatgaac caccggcaat tccgtttcct gttcgacgtc gatttcgatt gaactgatca
     6421 cggaaaataa acgcgaaaca ggaaacacaa gtacagacac catgctccgc cgcgattaca
     6481 tacagcgaat gatcgaagaa tacgggcgcg tcctcagccg gatcatggga cttacagatc
     6541 cttccgcact tgggaaagcg ctcggcgaga tacgggaatc ctacctcact tatttcgggg
     6601 tggacgccgc cgtcattgat aaatttcacc cggacgatct cccgcttttt cttacgaaag
     6661 aacatcattt tacattcgga cagctggaag cgctggccaa gggaattacg gcagaaggaa
     6721 atctttcccc ggatgaaatg gttgcaaaaa tgaaatacag gaaagcgctc gcgctttacc
     6781 tctggctcga gaaagaagac acgcagacct tttcgatcca ccgcaaacag gcggtgaacg
     6841 atcttatgaa taggatcggg cagtgacgtt gtgatcgctg cagattttgt gcggattcat
     6901 tgagaatacg gtaatcattt actcccggct gtaacttttc attgggagca gaattattac
     6961 atttgtgtaa cccacataaa acgtacttta tgtccagatt tcttcgcctc ccggcgctcc
     7021 ttctcctttt acttccggcc tttgtaatgg gtcagcagaa cgcagagctc gttaattcgg
     7081 gtcagcttct ttttgaagga aacctttact attatctcgg taaatacggc aaagccgaag
     7141 aaacattcaa gcgcgtcgga cggaatgata ccaattacgc ggttatcacg cgcgatcttg
     7201 cacgcacata cagtgacgac gacgaggaca gcctctgcct gatgacctgc gtgaaaggac
     7261 tcgaccttca ttcggtgtac gacgtggatt tttacaacta catgggcatt tccctcaagg
     7321 aaatgaaccg gtacgattcg gcgctgaaag tgtacgacaa ggccatcggc ctgttcccgt
     7381 atgcgcacct gctgtattac aacaaaggca tggtgtatta caagcagaaa aattacgtgg
     7441 aagcgcagaa gtggttccag gaagccgtac gtatcaatcc ctattattca caaagtcatt
     7501 tcatgctggg gaaatcgtgc gcggagcagg ggcgcatcat cccggccgtg ctgtcgttcg
     7561 aatatttcct gatggtcgat ccgtcttcgg acaaagcgcc aaaggtggtg acgacgatcg
     7621 aggatcttgt cacgggcgat taccagccgg atcccgacca gaagctggat gatgatgagt
     7681 ccggcgataa atgcttcgcc gacgtgaccg atgcgatcaa ttcgcagacc gcgcttcagg
     7741 cgggctaccg gaacaaaacc ggcatcaacc tgaaagtggt gaagcagctg cagtatcttt
     7801 ttgaaaagct gaagtaccag gaaggaacga acaactggtg gatggatttt tatgtgccgt
     7861 tctacacgga gctccagt
//
LOCUS       DASTSF010000058         6760 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_852267,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000058 DASTSF010000000
VERSION     DASTSF010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 6760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6760
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_852267"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1216
                     /locus_tag="VFU15_01790"
     CDS             <1..1216
                     /locus_tag="VFU15_01790"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEU4716528.1"
                     /translation="RIFHHPTRNKFYVVYAARHYNVAQPPGMLQEFRWREYDNTMTFT
                     GNEGVLPGPPGAGDYAMVMVDTNYYHLTNYGNTATGTLQYKLSKLDDNFNLISSVVIT
                     LDPHDSNIDQLMNYTNGKLIIGAMNDTCCTPPTTPPQPSYHPKVHLFQYDLNLNPVAP
                     DQVLPPQAYSWGASCIFNNNNYYIVSTDDIGTHVLYCYTYDANFNYISTTQINPNGQW
                     SQGILWDGTYYYVSFHSGDHNHGNVAIAVYDANWNQVLFQPVTNNPYSAGLNAHRPVM
                     CRVGNLLYVSYDLETYNTSTGANNKDWQAHVDAFQVNTPASVENFAASLYTMNLFPNP
                     AQGTITISTRNFSAGPKTIEVSDLSGRMVGQLEFDEENYTLSLYALKPGIYFVKVSDA
                     ENNAVVRKMVVR"
     gene            1284..3644
                     /locus_tag="VFU15_01795"
     CDS             1284..3644
                     /locus_tag="VFU15_01795"
                     /inference="COORDINATES: protein motif:HMM:NF025092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU4716529.1"
                     /translation="MKRLLTLAFFFLAAFSFAQVPTQTVRGTVVDNETKSELVGVIVL
                     IADTGQAPRNTTTDVNGGFRFENVAVGRHTLQFKYIGYKDVYITVIVSSGKEVVLNVE
                     MEESVVQQQEVVITDDANKGKPLNDMSTVSARSFTIEETQRYAGSLGDPSRMASNYAG
                     VSGADDSRNDVVIRGNSPLGLLWRLNGADIPNPNHFGSFGSTGGPVSMLNNNVLDNSD
                     FLTSAFPAEYGNALAGVFDLRMRNGNNEKFEFIGQVGFNGFEGGMEGPFSKKHRASFL
                     INYRYSTLAVFQKLGEQFGIGDAVPKYQDLSYKLMFPTHKLGDFSFWGVGGLSYIELL
                     DSKRDTTKLDLYTIGGFDTYYGTRSGVTGFTHSYIFSSDAYSRLNIAVSGMQNYIKLD
                     SVSFVDHTFWRSYGSDFRQAKLTMNYTFLKKFSAKNTLKSGVYADNIFINLRDSTSYG
                     VYYFRRIRDIKDHTWLLQAYSQWQHHFTDRFTFNLGIHSQVLLLNNSWSVEPRLGAKY
                     QLNEKQSVSCGAGMHSQMQTVFTYFNQTLLPDGTYYLSNSNIDFSRAVHAVVSWDWNF
                     SKNFRLKAEAYYQYLYSIPGLQRSTIYSGLNEGADFDAPGIDSLVNNGKGRNYGVELT
                     VEKFYSKGFYFLYTTSLYKAYFTPSDGKERQSAFSGNYVVNLLGGKEFHLNAKNTIAL
                     DLKLNAAGGKRYIPIDLAASGLAGEAVYDYSQAYDKRYPGYFRMDGKIGFIRNGKHIT
                     QQWSIECLNFTNHKNVFTQDYNAKLNQVVTNYQTGFLLVPSYKITF"
     gene            complement(3745..4716)
                     /locus_tag="VFU15_01800"
     CDS             complement(3745..4716)
                     /locus_tag="VFU15_01800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716530.1"
                     /translation="MIFLFLLFPYSAFAQMFLPVDDDPFGVPVKFNPDSVRHHKIKSI
                     TCSLQYKPDEHIIEDKGLKEYYQFDSLGRMEFYWRTRVHDYSQRAVDHPAVYRHGRMV
                     ARAYTEYKYMYTYDTIFVNYYYDNYQRLVCRRECDGDYYHTWYYIYNTDGTISRQTHC
                     RESNVGLGHANFRLGVQTIISQEDFSYQKFSSTQIKQLCLNDEGKPYKTTMMILDEKG
                     RPLDIREEYMSGGVRIVTTWKYDSLDRTSGYTYTSNAGSEFTENIQYNYDSLGRMASV
                     MRYQDGNLKDEFNYLYNGNSQVAYAYINRRHIEKAIDIVKMEVVYWG"
     gene            complement(4785..>6760)
                     /locus_tag="VFU15_01805"
     CDS             complement(4785..>6760)
                     /locus_tag="VFU15_01805"
                     /inference="COORDINATES: protein motif:HMM:NF015168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEU4716531.1"
                     /translation="GEEALRKAKEENRLMLVSIGYSACHWCHVMERESFEDEKVARIM
                     NDHFVCIKIDREERPDIDQVYMNAVQLMTSHGGWPLNCFTLPDGRPLYGGTYFPKEKW
                     VDTLFALADLWKNDAEKCMQYAQELTQGVKHSELPVADESAPAIAGNILGLSVEQWSL
                     RFDNSEGGPSRAPKFPLPNNYLFLLRQAFFTQSEKIKKHVLLTLDKMMMGGIYDQAGG
                     GFARYSTDMLWKVPHFEKMLYDNAQLISLYAEAFRWTKNEAYKNVVHETAEFAERELS
                     SPSGAFYSALDADSEGEEGKFYVWEREEMEKLLGEDFGLAADYFSLGNLSGGKSGMAY
                     WEDGKYILLRRESDEAFARKRHMEVTALQKKISGIKNVLMAQRAKRIRPGLDDKTLAS
                     WNALMLRGLVDAYQMEGEEKFLLRAKKNAEFLLREMLRMEDGKVTGMWHSWKNGRATV
                     NAFLEDYAFVTDAFIALYQAQFEEKWLMLAIALCEYACAHFGHEGSPLFCFTSDEDPA
                     LIARKMEISDNVIPASNSAMARNLFYLGHYTGRTEWIARAEQMLALVQKEIVAYGAGY
                     SNWMILQQHFVFPFREIAIVGNAVDKTGAAFRKHYLPNQIFAGSTGNSTLPLTANRFI
                     EGRTLIYVCENNTCGLPVAAAEEALKNIPGYAG"
BASE COUNT         1591 a         1949 c         1668 g         1552 t
ORIGIN      
        1 ccgcattttc catcatccca cgcggaataa attctacgtc gtgtacgcag cgcgtcatta
       61 caacgttgcg cagcctcccg gaatgctgca ggaattccgg tggcgggaat atgacaatac
      121 gatgacgttc accggcaacg aaggcgtact tcccggtcca cccggcgcag gcgattatgc
      181 aatggtaatg gtggatacga actattacca cctcacaaac tacggaaaca ccgccacggg
      241 aacattgcag tacaagctga gcaagctcga tgataatttc aatctcatca gcagcgtcgt
      301 aatcacgctc gacccgcatg attccaacat cgaccagctg atgaattaca ccaacggcaa
      361 gctcatcatc ggcgcaatga acgatacctg ctgcacgccg cccaccacac cgccgcagcc
      421 cagctatcac ccgaaagttc accttttcca gtacgacctt aaccttaatc ccgttgcacc
      481 cgaccaggtc cttccgccac aggcgtattc gtggggagcc agctgcatct tcaacaacaa
      541 caattactac atcgtttcca ccgacgacat tgggacacat gttctctatt gctatactta
      601 cgatgcgaac ttcaattaca tcagcaccac gcagatcaat cccaacgggc aatggtcgca
      661 gggaattttg tgggacggca cgtactatta cgtttctttc cattcgggcg atcacaacca
      721 cggcaatgtc gcgattgccg tttacgatgc gaactggaac caggtgctgt tccagccggt
      781 gacgaacaat ccttacagcg cgggactgaa cgcgcaccgc ccggtgatgt gccgcgtggg
      841 caacctgctg tacgtttcct acgaccttga aacatataac acatcgaccg gggcgaacaa
      901 caaagactgg caggcgcacg tcgatgcgtt ccaggtgaac acgccggcgt cggtggaaaa
      961 tttcgcagcg tcactttaca cgatgaacct ttttcccaac cccgcgcagg gtacgataac
     1021 catcagtacc cggaattttt ccgccggacc gaaaacgatc gaagtgagcg atctttccgg
     1081 gagaatggtc gggcagctgg aattcgatga agaaaactac acgctgagtc tttacgcttt
     1141 gaaacccggc atttacttcg taaaagtgag tgacgcggaa aacaatgcgg tggtgcgcaa
     1201 gatggtggtg agataacccc gaaagtgagc agcaaccgca tgatttgaat tcaattcatg
     1261 cggctttcct acttttgcac cgcatgaaac gcctgcttac ccttgcattt ttctttctgg
     1321 ccgcgttttc cttcgcgcag gtgcccacgc agaccgtgcg cggaacggtc gttgacaatg
     1381 aaacgaaaag cgagctggtg ggcgtgatcg tgctcatcgc cgataccggg caggcgccgc
     1441 gcaacaccac aaccgatgtg aacggcggat tccgttttga aaatgtcgcg gtgggaaggc
     1501 acaccttgca attcaaatac atcggctata aggatgttta catcacggtg atcgtgagct
     1561 cggggaaaga agtcgtgctg aacgtggaaa tggaagaatc cgtggtgcag cagcaggaag
     1621 tggtgatcac cgacgacgcg aacaaaggaa aaccgctcaa cgacatgagt accgtgagcg
     1681 cgcgttcgtt caccatcgag gaaacacagc gctacgccgg cagtctcggc gatccttccc
     1741 gcatggcttc caactatgcg ggcgtgagcg gcgccgatga ttcgcgcaac gatgtcgtca
     1801 tccgcgggaa ttcaccgctc ggacttttgt ggcggctcaa cggcgcggac attcccaacc
     1861 cgaatcactt cggctccttc ggcagcaccg gcggtcctgt gagcatgctc aacaacaacg
     1921 tgctcgacaa ttccgacttc cttacaagcg ccttccccgc agaatatggc aacgcgctcg
     1981 ccggcgtgtt cgacctgcgc atgcgcaacg gcaacaatga aaagttcgag ttcatcggcc
     2041 aggtgggctt caacgggttc gaaggcggaa tggaaggccc cttctcgaaa aagcaccgcg
     2101 catcgttcct catcaattac cgttactcca cgcttgccgt tttccagaag ctcggcgaac
     2161 agttcggcat cggcgacgcc gttccgaaat accaggacct ttcgtacaag ctcatgttcc
     2221 ccacgcacaa gctcggcgac ttttccttct ggggcgtggg cggattgagc tacatcgaac
     2281 tgctcgacag caagcgcgac acgacgaagc tcgatcttta caccatcggc ggtttcgata
     2341 cctactacgg aacacgcagc ggtgtaacgg gattcacaca cagctatatt ttttcctcgg
     2401 acgcctacag ccgcctgaac atcgccgtga gcggcatgca gaattacatc aaactcgatt
     2461 ccgtttcttt cgtcgatcac acgttctggc gcagctacgg cagcgatttc cgccaggcga
     2521 agctgacgat gaactacacg ttcctgaaaa aattcagcgc gaagaacacg ctgaaatccg
     2581 gcgtgtatgc cgacaacatc ttcatcaacc tgcgcgacag cacgagctac ggcgtttatt
     2641 atttccgccg catccgcgac atcaaagacc atacgtggct gctgcaggcg tattcgcaat
     2701 ggcagcacca tttcaccgac cgcttcacgt tcaacctcgg tatccattcg caggtcctgc
     2761 tgctgaacaa ttcgtggagc gtggagccgc gtctcggcgc gaaataccag ctgaatgaaa
     2821 aacaatccgt cagctgcggc gcaggcatgc acagccagat gcagaccgtg ttcacgtatt
     2881 tcaaccagac attgctgccg gacgggacgt attacctctc gaacagcaac atcgattttt
     2941 cgcgcgccgt gcatgcggtc gtttcctggg actggaattt ctcgaagaat ttccgcctga
     3001 aagctgaagc ctattaccag tatctctatt ccattcccgg gctgcagcgc tccacgatct
     3061 attcaggact caacgaaggc gccgacttcg atgcgcccgg catcgacagc ctcgtgaaca
     3121 acggcaaagg aagaaactac ggcgtggagc tgacggttga aaaattctac agcaagggat
     3181 tttatttcct gtataccact tcgctgtaca aagcgtattt cacacccagc gatggaaagg
     3241 aaagacaatc cgccttcagc gggaactacg tcgtgaatct cctcggcgga aaagaattcc
     3301 atctcaacgc gaagaacacc atcgcgctcg acctgaaatt aaacgcagcg ggcggaaagc
     3361 gatacatccc catcgatctc gccgcatcgg gactggcggg cgaagcggtt tacgattatt
     3421 cgcaggcgta cgacaaacgt taccccggct atttccgcat ggacgggaag atcggtttca
     3481 tccgcaacgg gaaacacatt acgcagcaat ggtcgatcga atgcctgaac ttcacgaacc
     3541 acaaaaatgt tttcacgcag gactacaacg caaagctgaa ccaggtggtc acgaattacc
     3601 agacgggatt cctgctcgta ccgagctata aaatcacttt ctgaaaacag ccggaaattc
     3661 tgaagccgaa atgagccgaa gtatttcctg atcccccgaa tcttttcgtt tcctgctttt
     3721 caactttgca actcttccgc tcactcatcc ccaatagacc acttccatct tcacaatatc
     3781 gatcgccttt tcaatgtgcc gccggttgat gtacgcatac gctacctgtg aatttccatt
     3841 gtagaggtaa ttgaactcat ctttcaggtt cccgtcctgg taacgcatga cagaagccat
     3901 tcttccgagc gaatcataat tgtactgtat gttttcggtg aattcggatc cggcgttcga
     3961 tgtgtacgta taacccgacg tacggtcgag cgaatcgtat ttccacgtcg tgacgatgcg
     4021 cacaccgccg ctcatgtatt cttcgcggat gtcgagcggg cggccttttt catcgaggat
     4081 catcatcgtc gttttataag gcttgccttc gtcattcagg caaagctgct tgatctgcgt
     4141 ggaagaaaat ttctggtagg aaaaatcttc ctgcgaaatg atcgtctgca cgccgaggcg
     4201 gaaattcgca tgaccgagcc ctacgttcga ttcccggcaa tgcgtctgcc gcgagatcgt
     4261 tccgtcggta ttgtagatgt aataccaggt gtggtaataa tcaccgtcgc attcccgcct
     4321 gcacacgagg cgctggtaat tgtcgtaata ataattcacg aagatcgtat cgtacgtgta
     4381 catgtatttg tattccgtgt aggcgcgcgc caccattctt ccgtgccggt aaaccgcggg
     4441 atgatcgacc gcgcgctggg aataatcgtg cacacgcgtg cgccagtaga attccatgcg
     4501 gcccagcgaa tcgaactggt agtattcttt caatcccttg tcctcgatga tatgctcatc
     4561 gggcttgtac tgcagactgc acgtgatgct ttttattttg tggtgacgga cactgtcggg
     4621 attgaatttc accggaacgc cgaacggatc atcgtctaca ggaaggaaca tctgtgcaaa
     4681 agcggaatac gggaacagca ggaaaagaaa aatcacggcg ggagcgctta tgttgcacct
     4741 cagaaaattg accggctgta ctcctgaaaa tcgtttccgt tctttcatcc cgcgtagccg
     4801 ggaatgtttt tcagcgcttc ttcagctgca gcgacaggca atccgcaggt gttgttttca
     4861 caaacataaa tgagcgttcg cccttctatg aacctgtttg cagtcagcgg cagcgtggaa
     4921 tttcccgtgc ttcctgcaaa gatttgattt ggaaggtaat gcttccggaa ggcggctcct
     4981 gttttatcaa cagcgttacc aacaatcgcg atctcgcgga acgggaaaac gaaatgctgc
     5041 tgcaggatca tccagttgga atagcccgcg ccgtaagcga caatttcctt ctgcacgagc
     5101 gcgagcatct gctccgcacg cgcgatccat tccgttcttc ccgtgtaatg accgaggtaa
     5161 aacaggttgc gcgccatggc ggaattcgaa gcggggatga cgttgtcgga aatttccatc
     5221 ttccgcgcaa tgagcgccgg atcttcatcg ctcgtaaagc agaacagcgg cgatccttca
     5281 tgaccgaaat gcgcacaggc gtattcgcag agcgcgatcg ccagcatgag ccatttctct
     5341 tcgaactgcg cctggtacag cgcgatgaac gcatcggtga cgaacgcgta atcctccagg
     5401 aaagcattca ccgtcgcccg gccgttcttc caggaatgcc acattcccgt cactttcccg
     5461 tcttccatcc gcagcatttc acggaggagg aattccgcat tttttttcgc gcgcagcagg
     5521 aatttttctt cgccttccat ctggtaagca tcgaccagcc cgcgaagcat gagcgcattc
     5581 cacgaagcga gcgttttgtc gtcgagaccc gggcggatgc gtttcgcacg ctgcgccatc
     5641 agcacgtttt ttatcccgct tattttcttt tgcagcgctg taacctccat gtggcgcttc
     5701 cgggcaaaag cttcatcgct ctcacgccgc agcaaaatat atttcccgtc ttcccagtac
     5761 gccatgccgc tttttccgcc ggaaagattt cccaggctga aataatccgc cgcaagcccg
     5821 aaatcctcac ccagcaattt ttccatttct tcccgttccc acacgtaaaa ttttccttcc
     5881 tcgccttccg aatcggcatc cagcgcggaa taaaacgcac ccgaaggcga cgacaattcc
     5941 cgctcggcga attcagctgt ttcatgcacg acatttttat acgcttcatt cttcgtccag
     6001 cggaacgctt ccgcatacag cgaaatgagc tgcgcattgt cgtacagcat tttttcgaag
     6061 tgcggaactt tccagagcat gtcggttgaa taacgcgcga atcctccgcc cgcctggtcg
     6121 taaatcccgc ccatcatcat tttatcgagc gtgagcagga cgtgtttttt tattttttcg
     6181 ctttgcgtga agaacgcctg gcgcagcagg aaaagataat tgttcggcaa cgggaatttc
     6241 ggcgcgcgtg aaggaccacc ctcgctgttg tcgaagcgca gcgaccattg ctccacggaa
     6301 agcccgagaa tatttccggc gatcgccggg gcggactcat ccgccacggg cagctccgaa
     6361 tgcttcacac cctgcgtgag ctcctgcgcg tactgcatgc atttttccgc gtcattcttc
     6421 cacagatctg caagcgcgaa cagcgtgtcg acccattttt ccttcgggaa atacgttccg
     6481 ccgtaaagcg gtcttccatc cggaagtgtg aaacagttca gcggccagcc tccgtggctg
     6541 gtcatcagct gcaccgcgtt catgtacacc tggtcgatgt cgggacgctc ttcccggtcg
     6601 atcttgatgc agacgaaatg atcattcatg atgcgcgcca ccttttcatc ttcgaacgat
     6661 tcgcgttcca tcacgtggca ccagtggcag gccgaatagc cgatgctcac cagcatgagc
     6721 cggttctcct ctttcgcttt gcgcagcgcc tcttcgcccc
//
LOCUS       DASTSF010000059         2822 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_852020,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000059 DASTSF010000000
VERSION     DASTSF010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2822
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_852020"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..570)
                     /locus_tag="VFU15_01810"
     CDS             complement(<1..570)
                     /locus_tag="VFU15_01810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716532.1"
                     /translation="MKKFLIVSALALSGTTAFSQIDNGVPSATGKGGASTALLRNWDA
                     IGINPANLGWADNYKFSVTVANFGLTAQSDAFQSKTLRNALISPNDTFNSADKQLYVK
                     EFDSPNALNFQANVNWGAASFYFPKIGGFAIGVRDRMWGHVGLNQNASDLIFNGINAA
                     VLQDTNTYHKTLGQILDSTNISYLHYRELN"
     gene            643..1050
                     /locus_tag="VFU15_01815"
     CDS             643..1050
                     /locus_tag="VFU15_01815"
                     /inference="COORDINATES: protein motif:HMM:NF025494.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4296 domain-containing protein"
                     /protein_id="HEU4716533.1"
                     /translation="MRFRLLAVFVLLVASCSHKGNVADRKPDDLIPEDKMIGVLVDVH
                     LLEASLVSNSVGPLPRPQAGNPGQVNMPVVQEQIIAKKIPYYDVFKKNGVTQAQYESS
                     LRWYTQHPDVLDKMYDAVIEQLVRRQAAVQAGK"
     gene            complement(1108..2115)
                     /locus_tag="VFU15_01820"
     CDS             complement(1108..2115)
                     /locus_tag="VFU15_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010665136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEU4716534.1"
                     /translation="MILLTGGTGLVGSHIAYELAKQGKQLRALKRKQSTTAAIEKIFR
                     HYAGNDAEKLLGRVEWVEGDLLDVGSLEDALEGVTHVFHAAAMVSFLPADRKKIVSFN
                     IDGTANLVNLSLDAGVKKFCHVSSIAALGKSVNGKIVTEDTWWKTDSSNSWYAISKYG
                     AEREVWRGTQEGLDAVIVSPSMILGPGDGERSSTQLFSALRKGLKWYTDGVNGYVDVR
                     DVAAAAVKLMFSEIKNERFILSAENLSYRAFVDKVLKNFNLPVTKFRAGKFLTGIGWR
                     AEKFLSVFSGKQPRLTPETTAASHEKNYYDGNLITRRTDFTYRNMDETIREVCGYYCE
                     T"
     gene            2221..>2822
                     /gene="tyrS"
                     /locus_tag="VFU15_01825"
     CDS             2221..>2822
                     /gene="tyrS"
                     /locus_tag="VFU15_01825"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009577445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEU4716535.1"
                     /translation="MKKNFIEELRWRRMLQDVMPGTEELLNREMVRGYIGFDPTADSL
                     GVGNMVQIMTLLHFQNCGHKPIALVGGATGMVGDPSGKSAERNLLSEDILRHNLDCQK
                     RQLEKFLDFNCGENSAEIVNNYDWFKNMSFLEFIRDTGKHITVNYMMAKDSVKNRIAG
                     EDREGMSFTEFTYQLVQGYDFYYLWKNRGVKLQMGGSDQWG"
BASE COUNT          621 a          746 c          776 g          679 t
ORIGIN      
        1 attcagctcg cggtaatgca ggtaggaaat gttggtgctg tcgaggattt gccccagcgt
       61 cttgtggtaa gtgttcgtgt cctgcagcac agcggcgttg atcccgttga aaataagatc
      121 cgatgcattc tggttcagtc cgacgtgtcc ccacatgcgg tcgcgtacgc cgatcgcaaa
      181 tccgccgatc ttcgggaagt agaacgatgc agcgccccag ttcacgttgg cctggaaatt
      241 cagcgcattc ggtgaatcga attcttttac ataaagctgc ttgtcggcgg aattgaatgt
      301 atcgttcgga ctgatcaatg cgttgcgcag cgttttcgac tggaaagcgt ccgactgcgc
      361 ggtaagaccg aaattcgcga cggtgactga aaatttgtag ttgtcagccc agcccaggtt
      421 ggcggggttg ataccgatcg catcccagtt gcgcagcagc gccgttgacg cgcctccttt
      481 ccccgtggcg gaaggaactc cgttgtcgat ctgcgaaaag gcggttgtgc ctgacaaggc
      541 aagcgcggaa acgatcagga attttttcat ggtcagtgtg tttaggtata ttaacaaaaa
      601 tatcgctttt ccccgacgtt tcgtgttcct accttagtgc ccatgagatt tcgcctgctt
      661 gctgtgtttg ttttgctcgt ggcatcgtgc agccataagg ggaatgttgc ggatagaaaa
      721 ccggatgatc tcatcccgga agacaagatg atcggtgtgc tcgtggatgt gcacctgctc
      781 gaagcttcgc tcgtgtcgaa ttctgtgggt ccgcttccgc ggccgcaggc cggcaacccg
      841 ggacaggtaa acatgcctgt tgtgcaggag cagatcatcg cgaaaaagat cccgtactac
      901 gatgttttca aaaagaacgg cgttacgcag gcgcaatatg aaagctccct gcgatggtat
      961 acgcagcacc ccgatgtgct cgataaaatg tacgacgctg tgatcgagca gctcgtccgc
     1021 aggcaggccg ctgtgcaggc agggaaataa ttaatgatga tcgatgaatg atcagggaga
     1081 gaacgatccc gccggcaagt ctcctgctca cgtttcacaa tagtagccgc acacttcccg
     1141 tatcgtctca tccatgttcc tgtacgtaaa atccgttctc cgcgtaatga gatttccgtc
     1201 gtaatagttt ttctcgtgtg atgcggctgt tgtttcggga gtaaggcggg gctgttttcc
     1261 tgaaaagacc gaaaggaatt tctccgctct ccaccctatg ccggtgagaa atttccccgc
     1321 acggaatttc gtcacaggga ggttgaaatt tttcagcact ttgtcgacaa atgcacggta
     1381 agaaagattt tccgcgctga ggataaagcg ttcgtttttt atttcgctga acatgagctt
     1441 cacggcagcg gcagctacgt cacgcacatc cacgtaaccg ttcacgccat cggtgtacca
     1501 tttcagtcct ttgcgcagcg cggaaaaaag ctgcgtgctg ctgcgttccc cgtcgcccgg
     1561 acccaggatc atggacgggc tcacgatgac ggcatcgaga ccctcctgtg ttccgcgcca
     1621 cacttcgcgc tccgcgccgt acttgctgat cgcgtaccag gaattcgaag aatcggtttt
     1681 ccaccacgtg tcttccgtta caattttccc gttcacgctt tttcccagcg cggcgatgga
     1741 gctgacgtga cagaattttt tcacaccggc gtccagcgaa aggttcacga gattggccgt
     1801 accgtcgatg ttgaacgaaa cgattttttt acggtccgcc ggaaggaagg acaccatcgc
     1861 ggccgcgtgg aagacatgcg tcactccttc gagcgcatct tccagcgaac ccacgtcgag
     1921 cagatcgcct tccacccatt cgacccggcc cagcagcttt tcagcgtcat tcccggcgta
     1981 atggcggaat attttttcga tcgcggcggt cgtgctttgc tttcgcttca gcgcacgcaa
     2041 ttgctttccc tgcttcgcca gctcgtacgc gatgtgcgaa ccgaccagtc ccgttccgcc
     2101 ggtgaggagt atcatgccgc aaaggtagcg cagttttcac gttccggctt ccgcttcccg
     2161 cgtatgtcgt cttacgtccg atgtctcacg actcacgcct aatctctacc tttgccacct
     2221 atgaaaaaga actttatcga agagctgcgc tggaggagaa tgctccagga tgtcatgccc
     2281 ggaacggagg aattgctgaa cagggaaatg gtgcgcgggt acatcggttt cgatcctacg
     2341 gccgactcgc tgggcgtggg caacatggtg cagatcatga cgctcctgca tttccagaat
     2401 tgcggtcata aaccgatcgc gctggtgggc ggagccacgg gaatggtggg cgatccttcc
     2461 gggaaatcgg cagagaggaa cctgctgtcg gaagatattc tccggcataa cctcgattgc
     2521 cagaaaagac agctggaaaa attcctcgat ttcaattgcg gggaaaattc ggcggagatc
     2581 gtgaacaatt acgactggtt caaaaatatg tcgttcctcg aattcatccg cgacaccggc
     2641 aagcacatta cggtcaatta catgatggcg aaagattcgg tgaagaaccg tattgccggc
     2701 gaagaccgcg aaggaatgtc gttcacggaa ttcacctacc agctcgtgca gggctacgac
     2761 ttttactatt tgtggaagaa ccggggcgtg aagctgcaga tgggcggctc ggaccagtgg
     2821 gg
//
LOCUS       DASTSF010000060         2811 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1745107,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000060 DASTSF010000000
VERSION     DASTSF010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2811
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1745107"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..719
                     /locus_tag="VFU15_01830"
     CDS             <1..719
                     /locus_tag="VFU15_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013664761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DALR domain-containing protein"
                     /protein_id="HEU4716536.1"
                     /translation="WIHTNMLTVNGQKMSKSLGNSFLPVELFTGQKLAADSRSANLTE
                     AKLDGKHPALDKGYSPMTVRFFMLQTHYSSTLDFSNEALQASEKGFRKLMEAVRILDK
                     LKAGEQSTFDVEGFRGRCTDAMNDDFNTPILIAQLFDAVRIINSVNDGKEFLTAKDIS
                     ALKKTMHDFVFSVLGLKDDMMESAGDLSEGLMQLIIRIRAEARAKKDFQTSDLVRDEL
                     GKLHIVLKDGKEGTGWELKS"
     gene            760..1761
                     /locus_tag="VFU15_01835"
     CDS             760..1761
                     /locus_tag="VFU15_01835"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEU4716537.1"
                     /translation="MKKIIIITAVTFFALASCTPDSPRSHEEDSAANNTRPEKQRVKG
                     PAFSGDSAYQYIKTQVGFGPRVPGTKAHDDCAAFITQKLKKAGLAVDVQEAQVVTFDG
                     KSIGIKNIFAAFHPERKDRILLMAHWDTRPWADEDPDSTAHKSSFDGADDGGSGVGVL
                     LEIANVLQQKDPNIGVDLLFDDAEDWGSNGGVSETWCLGTQYWTAHMPQNYTARYGIL
                     LDMVGGKGAVFPREGTGVQYAADIVEKVWSSAARLGYGGIFTNDITGETTDDHLFMNQ
                     NAHIPTIDIVHYNMQMHSYPPWHHKRSDTMDNIDTVTLNTVGAVLVDVIYNENPPVN"
     gene            1883..2470
                     /locus_tag="VFU15_01840"
     CDS             1883..2470
                     /locus_tag="VFU15_01840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716538.1"
                     /translation="MKKHLSLLLLFLAGSFGFSLHAELVWKPGYVILASGDSVKGDVH
                     VNTKKELNMFSRISFRQGEAQKTYKPEQVKEYGYDDVKFLARDVDGEKSFLKVLSYGR
                     IILFEYQYEVQRGNDLVVDSDYYMEKNDGSNSGLTKVKMGKFKKLAAEMMSDNTDLVQ
                     RVQNDDKKYEITDVQKVIDEYNSWYEQQNTLQGSR"
     gene            2586..>2811
                     /locus_tag="VFU15_01845"
     CDS             2586..>2811
                     /locus_tag="VFU15_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015810599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716539.1"
                     /translation="MQKKLFLLDAFALIYRSYFAFANNPRINSKGLNTSAVFGFTNTL
                     LEIIKKEQPTHIAVVFDTAEPTHRHTEFEAY"
BASE COUNT          725 a          736 c          743 g          607 t
ORIGIN      
        1 actggataca tacgaacatg ctgaccgtga acgggcagaa aatgtcgaag tcgctcggga
       61 attctttttt accggtggag cttttcaccg ggcagaagct cgccgccgat tcgcgttccg
      121 ccaatctcac cgaagcgaag ctcgacggga aacatcccgc gctggacaaa ggctattcgc
      181 cgatgacggt gcgcttcttc atgctgcaga cgcattattc cagcacgctt gatttttcca
      241 acgaagcgct gcaggcgtct gaaaaaggtt tccggaagct gatggaagcc gtgcgtattc
      301 tcgacaagct gaaagcgggc gaacaatcga ccttcgatgt tgagggtttc cgcggacgct
      361 gcacggacgc gatgaacgac gatttcaaca cacccattct catcgcgcag ctgttcgatg
      421 ccgtccgcat catcaattcc gtgaacgacg ggaaagagtt tcttaccgcg aaagacattt
      481 ccgcgctgaa aaaaaccatg catgattttg tgttcagcgt gctcgggctt aaagacgaca
      541 tgatggaatc ggccggtgat ctttccgaag ggcttatgca gctcatcatc cgcatccgtg
      601 cggaggcgcg tgcaaaaaaa gatttccaga cgtccgacct cgtccgcgac gagctgggaa
      661 agctgcacat cgtgttaaaa gatggtaagg aaggaacagg ctgggagctc aaatcgtaaa
      721 ttacatcgtt tcccggaggg aaaatcctgc gaatacacaa tgaaaaagat catcatcata
      781 accgcggtta ctttcttcgc gcttgcttcg tgcacgcccg acagcccgcg ctcgcacgaa
      841 gaagattcgg cggcgaacaa tacgcggccg gaaaaacaac gcgtgaaagg tcccgccttc
      901 tccggcgatt ccgcttacca gtacatcaaa acacaggtgg gattcggtcc gcgcgtgccc
      961 ggaacgaaag cgcacgacga ctgcgccgca ttcatcaccc aaaagctgaa aaaagcgggc
     1021 ctggcggtag acgtgcagga agcgcaggtg gtgacgttcg acggaaaatc gatcgggatc
     1081 aagaatattt tcgctgcgtt ccacccggag cgcaaggacc gcattcttct catggcgcac
     1141 tgggacacgc gcccgtgggc cgatgaagat ccggacagca cggcacacaa aagctcgttc
     1201 gacggagcgg acgacggcgg aagcggtgtc ggtgtgctgc tggagatcgc gaacgtgctg
     1261 cagcagaaag acccgaacat cggcgtcgat cttctttttg acgatgcaga agactggggc
     1321 agcaacggcg gcgtaagcga aacgtggtgc ctcggcacgc aatactggac ggcgcacatg
     1381 ccgcagaatt atacggcgcg ttacggcatt ctcctcgaca tggtgggcgg caagggagcc
     1441 gttttcccga gggaaggaac cggtgtccag tatgcagcgg acatcgtgga aaaagtatgg
     1501 agctcagcgg cgcgtttggg ctacggtggc attttcacca atgacatcac gggagaaacg
     1561 accgacgatc atcttttcat gaaccagaac gcgcacatcc ccacgatcga catcgtccac
     1621 tacaacatgc agatgcattc gtatcccccc tggcatcaca agcgcagcga tacgatggac
     1681 aacatcgata cggttacgct gaacaccgtc ggtgcggttc tcgtcgatgt gatctacaat
     1741 gagaatccgc cggtgaactg aaaccgggtt aaggttttat tcacaaaaac aatgcagttt
     1801 tcaacatttt cgcagagata ctaacacgct ctttcatttg cggcaggcat gtcttttctt
     1861 tgattccctg accagcaaaa aaatgaaaaa gcacctctct ctcctccttc ttttccttgc
     1921 cggatcattc gggttttcgc ttcacgccga gctcgtctgg aagccgggct atgttattct
     1981 tgccagcggc gattccgtca aaggcgatgt tcatgtgaac acgaagaaag agctgaatat
     2041 gttttccagg atctctttcc gccagggtga ggcgcagaaa acctacaagc ccgagcaggt
     2101 gaaagagtac ggctatgacg acgtgaaatt ccttgcgcgt gatgtggacg gtgagaaatc
     2161 ttttctcaag gtgctttcct acggacgcat catccttttc gaataccagt atgaagtgca
     2221 gcgcggcaat gatcttgttg tcgacagcga ttattacatg gaaaagaacg acggcagcaa
     2281 ttccggcctg acgaaagtga agatgggaaa attcaaaaag ctggcggctg aaatgatgag
     2341 cgataatacg gatctcgttc agcgcgtgca gaacgacgac aaaaaatacg agatcacgga
     2401 cgtacagaaa gtgatcgacg aatacaacag ctggtacgag cagcagaata cgctgcaggg
     2461 ttcacgctga agatttccct tagtagtagt ttccgccgac ggccctggag cagtaatgcg
     2521 tcagggctgt ctggtttttg ctcctgctca aataagattt aacttcgttt ctcatccttt
     2581 tcacaatgca gaaaaaactt tttctcctcg atgcttttgc gctgatctac cgttcgtatt
     2641 tcgcttttgc caataacccg cgcatcaatt caaaagggct caacacatcg gccgttttcg
     2701 gctttaccaa tacgctgctc gagatcatca aaaaagagca gcccacacat atcgccgttg
     2761 tgttcgacac ggcggaaccg acgcaccggc acaccgaatt cgaagcttat a
//
LOCUS       DASTSF010000061         3629 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_182194,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000061 DASTSF010000000
VERSION     DASTSF010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3629
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182194"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..138)
                     /locus_tag="VFU15_01850"
     CDS             complement(<1..138)
                     /locus_tag="VFU15_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013597595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine N-6-methyltransferase family
                     protein"
                     /protein_id="HEU4716540.1"
                     /translation="MIVRAKKHLGQHFLRDENIAKKIATSLTGFGNYHRVLEVGPGMG
                     VL"
     gene            complement(181..480)
                     /locus_tag="VFU15_01855"
     CDS             complement(181..480)
                     /locus_tag="VFU15_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018472274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4286 family protein"
                     /protein_id="HEU4716541.1"
                     /translation="MILYNVTVNIDDSVHDEWLKWMKETHIPDVVATGCFSSGTIFRL
                     LVEEQSGTSYSIQYRAPNIEAVKRYLGSFADKLRSEHTEKFRDKFTAFRTVLEEV"
     gene            complement(483..2342)
                     /locus_tag="VFU15_01860"
     CDS             complement(483..2342)
                     /locus_tag="VFU15_01860"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU4716542.1"
                     /translation="MKKLLHILLFLIAVPLFVAAQPPGGNTKKQDLSADEQLAAQYYQ
                     SGDYDKATVYYEKLYNSSPITLYYNYYLNCLIFTKDFKKAEKLVSKQQKRNPADLRGK
                     VDMGRVYQAEGEDDKAKKEFSAAIDGINSGTSANSVIDLANSFLGINETDYAISALLK
                     GRKELKGVYSFNMELAEIYNIKKDYQSMVNEYLGLLDISEGYRQSVQNNLQAKMDADQ
                     DNKIQPILKDELIKRAQKDPDKIIWPEMLMWLYMQQKNFSAALVQARAIDKRTTHDGS
                     NVMDLAATARANGEYDVATDAYDYVIGLGKNGDNYRKARTEKVNTKYDKITVAGNYTQ
                     ADLLALETEMNSTLDELGRDAGTVPLMRTLAHLEAFYLFKTKEAISILEQAIAMPGVT
                     PMDQANCKLELGDVLLLDGQVWEASLRYSQVEKAFKYEPIGEEAKFRNAKVAFYIGDF
                     KWAQVQLDVLKGATSRLISNDAMYLSLLITDNLALDSNPEPLLMFARADLLIFQNRFD
                     KATLELDSIDKKYPSHSLEDDVLYKRYEIADKQQKYTDAAGYLDQILQKYPTDILGDD
                     AMFGLAQLYDYKLNDKDKAKKYYLQLITDYPGSLYVVDARKRYRILNGDKIAN"
     gene            complement(2339..2932)
                     /locus_tag="VFU15_01865"
     CDS             complement(2339..2932)
                     /locus_tag="VFU15_01865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716543.1"
                     /translation="MKTTAHLLFGMIVAAFLGGCKGPAYDKELGEVSALRTKLDAADS
                     MLKGIDVEMTEKLAEETESNFSYIQVAIAKIGDTLDWKSALLLTNFQQVKEGFESVSS
                     NVDRLKAAIDSTRSNLENLDHDLKNNSLSRTLTPQDCIEQETEHVNAISEAIDELKEK
                     IEGRKKAYDTLSPKIQTYILQLNQRLNEKGQSPPPKK"
     gene            complement(2941..>3629)
                     /locus_tag="VFU15_01870"
     CDS             complement(2941..>3629)
                     /locus_tag="VFU15_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014681514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminoacyl--tRNA ligase-related protein"
                     /protein_id="HEU4716544.1"
                     /translation="VQPPILVNKDSGFGTGQLPDKEGQMYHVTVDDLYLIPTAEVPIT
                     NIYRDVILKAEELPVKNCGYTPCFRREAGSYGKDVRGLNRLHQFDKVEIVQVADPAKS
                     YRALEEMREFVAGLLKELELPFRTLLLCGGDMSFASAQTYDMEVYSAAQEKWLEVSSV
                     SNFETFQSNRLKLRFREGNEKPQPAHTLNGSALALPRIVAALLENNQVEDGILIPQAL
                     RSYTGFEKIN"
BASE COUNT          727 a          989 c          939 g          974 t
ORIGIN      
        1 gagcacaccc atgccgggac ctacttcgag cacgcgatgg taattgccaa aacccgtcag
       61 gcttgtcgcg atctttttcg caatgttctc atcgcgcagg aaatgctgac cgagatgttt
      121 tttggcgcga acgatcactg caaatgataa atttaaaatg atgaatgaat acaggaagga
      181 ttacacttct tccagcacgg tgcggaacgc ggtgaatttg tccctgaatt tttccgtgtg
      241 ttcgctgcgc agcttatcgg caaaactccc gaggtaacgc ttcacagcct cgatgttcgg
      301 ggcgcggtac tggatggaat agcttgtgcc gctctgctct tccaccagca ggcggaaaat
      361 agtcccggaa gaaaaacacc cggttgccac gacatccggg atatgcgttt ctttcatcca
      421 cttcagccat tcgtcgtgaa cggagtcgtc gatgttgaca gttacattgt aaagtatcat
      481 gatcagttgg cgattttatc cccgttgaga atgcggtaac gtttacgcgc atccacgaca
      541 taaaggctcc cgggataatc cgtgatgagc tgcaggtaat acttcttcgc tttgtctttg
      601 tcgttcaatt tgtaatcgta aagttgcgca agaccgaaca tggcatcatc gccgagaata
      661 tccgtaggat atttctgcag gatctggtcg aggtaaccgg ccgcatccgt gtatttctgc
      721 tgcttgtccg cgatctcgta gcgtttgtag agcacatcgt cctcgagtga atgcgaagga
      781 tatttcttgt cgatggaatc gagctcgagc gtcgctttgt cgaatcggtt ctggaaaatg
      841 agcaggtcgg cgcgcgcaaa catcagcagc ggttcggggt tgctgtcgag cgcgagatta
      901 tcggtgatga gcagcgaaag atacatcgca tcgttggaga tgagccggga ggttgcgcct
      961 ttcagcacgt cgagctgcac ctgcgcccac ttgaaatcac cgatatagaa cgccactttc
     1021 gcattgcgga atttcgcttc ctcgccgatc ggttcatact tgaacgcttt ttccacctgc
     1081 gaataacgga gcgaagcttc ccacacctgc ccgtccagca acagcacgtc gcccagctcc
     1141 agcttgcagt tcgcctggtc catcggcgta acgccgggca tcgcgatcgc ctgttcgaga
     1201 atggagatcg cctcttttgt tttgaaaaga tagaacgctt cgaggtgcgc cagcgtgcgc
     1261 atgagcggaa cggttcccgc atcgcggccc agctcgtcga gcgtggaatt catttccgtc
     1321 tcgagcgcga gcagatcagc ctgcgtgtag tttcccgcga cggtgatctt atcgtatttc
     1381 gtgttgacct tttccgtgcg cgccttgcgg taattgtctc cattcttgcc gaggccgatc
     1441 acgtaatcgt acgcatctgt tgcgacgtca tattccccgt tcgcgcgtgc cgttgccgca
     1501 agatccatca cgttgctgcc gtcatgcgtc gtgcgcttgt cgatcgccct ggcctgcacc
     1561 agcgccgctg agaaattctt ctgctgcatg tacagccaca tcagcatttc cggccagatg
     1621 atcttgtcgg gatctttctg cgcacgttta atgagttcgt ctttcaggat cggctggatc
     1681 ttgttgtcct ggtcggcgtc catcttggcc tgcaggttgt tctgcaccga ttgcctgtaa
     1741 ccttcggaaa tatcgagcag cccgaggtat tcattcacca tggactggta atcttttttg
     1801 atgttgtaga tctcggccag ctccatgttg aacgaataaa cgcctttcag ctccttccgt
     1861 cctttcagca gcgcgctgat cgcgtaatcg gtttcattga tgcccagaaa agaatttgca
     1921 agatcgatca cgctgttggc gcttgtcccg gaattgatcc cgtcgatcgc tgcggaaaat
     1981 tcttttttcg ctttgtcatc ttctccttcc gcctggtaca cgcgccccat gtccaccttg
     2041 ccgcgcaggt ccgccgggtt tcttttctgc tgtttggaaa cgagcttttc cgccttcttg
     2101 aaatctttcg tgaagatgag gcagttcagg tagtagttgt aataaagcgt gatcgggctg
     2161 ctgttgtaca gcttttcata atagaccgtc gccttgtcgt aatcaccgct ctggtaatac
     2221 tgcgctgcga gctgctcgtc ggcggaaagg tcctgctttt ttgtatttcc tcccggcggc
     2281 tgcgccgcaa cgaagagcgg aaccgcgatg agaaataaaa gtatatgcag gagctttttc
     2341 attttttcgg cggcggcgac tggccttttt cgttcaggcg ctggttgagc tgcagaatgt
     2401 acgtttgtat tttcggtgaa agcgtgtcgt atgctttctt acgtccttct attttttcct
     2461 tcagctcgtc gatcgcttcg gaaatggcgt tcacatgctc cgtttcctgc tcaatgcagt
     2521 cctgcggcgt caacgttctc gaaagcgaat tgttcttcag gtcgtggtcc aggttttcga
     2581 ggttcgatcg cgtcgagtca atggccgctt tcagccggtc gacattcgaa gaaacagatt
     2641 caaacccttc cttcacctgc tggaaattgg tcagcagcag cgcgcttttc cagtcgagcg
     2701 tatcgccgat cttcgcgata gccacctgga tgtagctgaa gttcgattcc gtttcttccg
     2761 ccagcttttc cgtcatttcc acatcaatgc ccttcagcat ggaatccgca gcgtccagct
     2821 tcgtccgcaa tgcgctcacc tcgcccagct ctttatcata cgcaggacct ttacatccgc
     2881 ccaaaaaagc agccacgatc atgccgaata aaagatgcgc cgtcgttttc atttccacat
     2941 tcagttgatt ttttcaaatc ctgtataaga acgcaaagcc tgggggataa gtatgccgtc
     3001 ttccacctgg ttgttctcga gcagcgcggc aacgatcctg ggtaaagcga gcgcgctccc
     3061 gttgagcgta tgcgcgggct gcggtttttc atttccttcc cggaaacgca atttaagacg
     3121 attgctctgg aacgtttcga aattggaaac agagctcacc tcgagccatt tttcctgcgc
     3181 cgcggaatac acttccatgt cgtaggtctg cgcggaagcg aagctcatgt cgccgccgca
     3241 cagcagcagc gtgcggaacg gaagctccag ctctttcaga agcccggcca cgaattcacg
     3301 catttcttcc aatgcccggt atgattttgc cgggtccgcc acctgcacga tctccacctt
     3361 atcgaactgg tgaaggcggt tgaggccacg gacatccttc ccgtaggatc cggcttccct
     3421 ccggaagcag ggcgtatagc cgcagttctt caccggaagc tcttcagctt tgaggatgac
     3481 gtcgcggtaa atgttcgtga tgggaacttc agccgtgggg atgagataaa gatcgtcgac
     3541 cgtcacgtgg tacatctgcc cttccttgtc gggaagctgg cctgtgccga agccggaatc
     3601 tttgttgacg aggatcggcg gctgcacct
//
LOCUS       DASTSF010000062         7823 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1970293,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000062 DASTSF010000000
VERSION     DASTSF010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 7823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7823
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1970293"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3498
                     /locus_tag="VFU15_01875"
     CDS             <1..3498
                     /locus_tag="VFU15_01875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716545.1"
                     /translation="KAGLDKQYNDLIADADKKFAASDWTNAKSQYQAASNLKTAEQYP
                     KDQMKKCDDNLAKADLDKKYGDFIADADKKFAASDWAGAKTAYQSASNLKASEQYPKD
                     QIKICDDNIAKAGLDKQYNDLIADADKKFAASDWANAKSQYQAASNLKASEQYPKDQV
                     KLCDDNIAKAGLDKQYNDLVKQADTKFKANDFAGAKTLYQQASDLKTSEQYPKDQIKL
                     CDDGIAKAGVDKQYNDLVKQADDKFKAADWSGAKDLYSRASGVKSSEQYPKDQMKKCD
                     DNIAKAGVDKQYSDLIADADKKFAAADWANAKTQYLAASDLKKAEQYPKDQVKKCDDN
                     IAKAGLDKQYSDLIADADGKFGASDWAGAKAQYQAASALKAAEQYPKDQMKKCDDNIA
                     KQGKDKKYNDLITSADEKFKAKDWAGAKSIYQQASAVKTDEQYPKDQVKACDDAVQKE
                     KDAAYNAIVAKADSLFGKSDWAGAKAIYQQASTSRPAETYPKDQMKKCDENIAKEGKD
                     KKYNDLITSADEKFKAADWAGAKTIYLQATAVKADEQYPKDQAKLCDDNIAKAGTDKK
                     YNDAIAAADQKFGAGDWSGAKAKYQDALAIKPGEQYPSDQVKKCDDNSAADLEKKYTD
                     AIKDADAKFGTQDFSGAKVLYQQASTLKPGEQYPKDQIKLCDDGLSKQGVDKKYNDLI
                     AQADTKFKASDWAGAKTLYLQASAVKDGEQYPKDQAKLCDQKIQDAGLDKQYNDLVKQ
                     ADAKFKLSDWAGAKAIYQQASDLKTSEQYPKDQVKLCDDNIAKAGDKKYNDAVAAADA
                     KFKASDWAGAKAGYNTALGMKPGEQYPQDQIKLCDDNIAKAGTDKQYNAAIADADAKF
                     KLKDWNGAKAKYNDALAIKGDEQYPKDQIKLCDDELNKLLGQDQAYKLAVAKGDSAFA
                     MNDYETAQTAYTNAKNLKPKEAYPPAQLAKIAGLIADMQKDKSYHDLLAKGDSLFNAK
                     DYDGAKKIYTTATTQKPSDQYPKDKLAEIKRIQDDIAFEQKKDAKYNKLIADADKKFK
                     AGDWKGARDLYSQALLEKSEKYPRDQIAACDKKLNPPPANTKDSVKVQTHDEYVNDLV
                     TKYPQGVTKMENQDGGTTTYTTIVVIGNQAWVYTKKVYSYGTYYFKDGEQISEATFNY
                     DTNPSYVDKRLNDLNNNQK"
     gene            complement(3590..4873)
                     /locus_tag="VFU15_01880"
     CDS             complement(3590..4873)
                     /locus_tag="VFU15_01880"
                     /inference="COORDINATES: protein motif:HMM:NF025034.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="retropepsin-like aspartic protease"
                     /protein_id="HEU4716546.1"
                     /translation="MKKFFFTLLPALLSVPLSASNGEAHTEKLFNLVNTGDYFHFREE
                     FLADSVNLDPLYRNYFSVWNNSLFNRPEISEAITRQLTDSTAMNCPDSLAEHALMLVF
                     QDKLRLSRFREADSICTHLLFRYGSIMDDDEKKGVREAGDLSALLKDIDPMTVERNGD
                     FSLDYKRDITNLIRIPVQINGHANDYVMDTGANFSAITETQAQEMGLDVRSGNFSITS
                     SSRSSVQARMGVAKEVVIGNAKFRNVVFIVLPDNSLRFAGGLYKINGIIGLPVLAALG
                     ELHIFRKKIVFPVAQEEGPGINLGFYGNTSFIDLSFFNDHSPFIFDTGAATSVFGPVA
                     LHKFSDALYTTGHGKDKTARIGGAGGVQKVHIIRCKNVAYEGGGKSGTLKMVTVQVDG
                     NTAAFGMFSGIAGEDILMQWNEVVINYRKMFVVFR"
     gene            complement(4880..5389)
                     /locus_tag="VFU15_01885"
     CDS             complement(4880..5389)
                     /locus_tag="VFU15_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014201159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HEU4716547.1"
                     /translation="MSSERTLVMNAGQIERRINRLAWQVYEHCFNEKEIIIAGIEPNG
                     LTLSERIAAVLRSISPLKVTVAALKVDKENPLEKSASVSLPEKDFSGKTIIVVDDVSN
                     SGRTLMYGVDFFLRFKIKSILTVVLVDRDHNRFPVKTNFVGLSLSTTMQEHITVEFGK
                     NGSDKVFLD"
     gene            complement(5469..>7823)
                     /locus_tag="VFU15_01890"
     CDS             complement(5469..>7823)
                     /locus_tag="VFU15_01890"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HEU4716548.1"
                     /translation="TCDGGPGVWQWFNNVNLPNGNGTQSGFFFDGDIVQGGPDGDPTN
                     NFGDNCTGNVNWTFCWTITANACPPGQNGDDLSINIDTYGDGETGNWTNVACSNDPIY
                     QFNASLACCAPPQIAQTNVTCFGGNNGSITATALGTGPFDYVWTDNSNNTIQTTLNQN
                     GPDVLNNLSAGTYHVTVTDHSNNCVTTTNIVITQPTQLVLNVASTPASCGQSNGSGTV
                     TPAGGTGPYDYVWTDSANNVIQTATNLPGSNTASNLPAGTYNVLVTDANNCLANISVV
                     VTGVPGNLAANITSTPTSCNGVCDGTATVTLNGGTAPYNYQWDANAANQVTATATNLC
                     AGTYTVNYSDANNCSGSATVQVTSPPPVTAVVAAAPSTICIGQSSTLTCTPGGGTGAG
                     YTYNWAPAGTGATQSVTVSPGTTQGYTVTVADGNGCASAPATVTVTVDPPLNINLNSA
                     PAAVCAGASSTLTAAGGGGNGGPYTYTWLPAGTAGNTTTANVTPATTTTYTVIISDGC
                     SPDDSAFTTVTVNPPPAPSFTGDPLTGCAPLCVNFMTPNNPSWVNCSWDFGDGNLGSG
                     NCNPSNCYANPGCYDVTLTVNDANGCTGTVTMPSYICVSENVVAQFTATPQPTTVFES
                     NITFTDESTGNPVSWTWYFGTLDSSNVQNPAYDFPNDQPGCYDVILVANNLDNCPGTD
                     TMRVCIDPEYSIYFPNAFTPNGDGINDGFIGVGQGIDKYEMWVFDRWGNMIYYTSDMS
                     KPWDGTVQGKSGNICQEDVYVWKATVVDVFGKKHKFIGHVSLVR"
BASE COUNT         2087 a         1951 c         2174 g         1611 t
ORIGIN      
        1 aaagcgggtc tcgacaagca gtacaacgac ctgatcgcgg atgcggataa aaaattcgcg
       61 gcgtcggatt ggacgaatgc aaaatcacag taccaggcgg cgtccaacct gaaaacggcg
      121 gaacagtatc cgaaggatca gatgaaaaaa tgcgatgata atctcgcgaa agctgatctc
      181 gataagaagt acggcgactt catcgcggat gcggataaaa aattcgcagc ttccgattgg
      241 gcgggtgcaa aaaccgctta ccagtcggca tcgaacctga aagcttcgga gcaatacccg
      301 aaggaccaga tcaaaatatg tgatgacaac atcgcaaaag cgggtctcga caagcagtac
      361 aacgacctga tcgcggatgc ggataaaaaa ttcgcggcgt cggattgggc gaatgcaaaa
      421 tcgcagtacc aggcggcatc gaacctgaaa gcttcggaac aatacccgaa ggaccaggtg
      481 aaattatgcg acgataatat tgcgaaagca ggactcgaca aacaatacaa tgatctcgtg
      541 aagcaggcgg atacgaaatt caaggcgaat gattttgcag gagcgaaaac gctgtaccag
      601 caggcgtctg acctgaaaac atccgagcag tacccgaaag accagataaa actgtgcgac
      661 gatggcattg cgaaagccgg cgtcgacaag caatacaacg acctggtcaa acaggccgac
      721 gacaagttca aggcagccga ctggtcaggc gcgaaagacc tgtacagccg ggcttcgggt
      781 gtcaagtctt cggagcagta tccgaaggac cagatgaaga aatgcgatga caacatcgcg
      841 aaggccggtg tcgacaagca atacagcgac ctgatcgcgg atgcggataa aaaattcgct
      901 gccgccgatt gggcgaatgc gaaaacgcaa tacctggccg cttccgatct gaaaaaagcg
      961 gaacaatacc cgaaagacca ggtgaagaag tgcgatgaca acatcgcgaa agcaggtctc
     1021 gataagcaat acagcgatct catcgccgat gccgacggca agttcggcgc ttcggattgg
     1081 gccggtgcga aagcgcaata tcaggccgcg tctgcgctca aggcggccga acaatacccg
     1141 aaagaccaga tgaaaaaatg cgatgacaac atcgcgaaac aaggaaagga taaaaaatac
     1201 aatgatctca ttacttcggc agatgaaaaa ttcaaggcga aggattgggc cggtgcaaaa
     1261 tcaatatacc agcaggcttc cgcggtaaaa acggatgagc agtatccgaa ggaccaggtc
     1321 aaggcgtgtg acgatgctgt tcagaaagag aaagacgcag cttacaatgc catcgttgcg
     1381 aaagcggatt cgcttttcgg gaagagcgat tgggccggtg caaaggccat ttaccagcag
     1441 gcatccactt cgcggccggc ggaaacctat ccgaaggacc agatgaaaaa gtgcgatgag
     1501 aacatcgcga aagaaggaaa ggataaaaaa tacaatgatc tcatcacttc agcagatgaa
     1561 aaattcaaag cggctgactg ggccggtgcg aaaacaattt atctgcaagc cactgcggtg
     1621 aaagcggatg agcaatatcc caaagaccag gcaaagctgt gcgacgacaa catcgccaaa
     1681 gccgggacgg ataaaaaata caacgacgcc attgctgccg ctgatcagaa attcggcgcg
     1741 ggcgactggt ccggtgcaaa agcgaaatac caggatgcgc tggccatcaa gcccggtgag
     1801 caatatcctt ccgaccaggt caaaaagtgc gacgacaata gtgcggctga tcttgaaaag
     1861 aaatacaccg atgcgataaa agatgcggat gcgaaattcg gcacacagga tttttccggg
     1921 gcgaaagtgc tttaccagca ggcttccacg ttaaagccgg gcgaacaata tccgaaggac
     1981 cagatcaaat tatgcgatga cggtctttcg aaacagggcg ttgacaaaaa atacaatgat
     2041 ctcattgcgc aggcggatac gaagttcaaa gcatccgact gggccggggc caaaacactt
     2101 tacctgcagg cttccgccgt gaaggacgga gagcaatacc ccaaagacca ggcgaagctg
     2161 tgcgatcaga agatacagga cgcaggtctg gataaacaat acaacgacct cgtcaaacag
     2221 gccgatgcaa aattcaagct gagcgactgg gccggcgcaa aagcgatcta ccagcaggcg
     2281 tccgatctga aaacgtcgga acagtatccg aaagaccagg tgaagctgtg cgatgacaac
     2341 attgcaaaag caggtgacaa aaaatacaat gatgcggtcg ctgctgccga tgcgaaattc
     2401 aaagcttcgg attgggcagg agcaaaggcg ggctacaaca ctgcgcttgg catgaagccc
     2461 ggtgagcagt atccgcagga ccagatcaaa ttatgtgatg acaatatcgc gaaagccgga
     2521 accgacaagc aatacaacgc cgcgatcgcg gatgcggatg cgaaattcaa gctgaaggac
     2581 tggaacggcg cgaaagcgaa atacaatgat gcgctggcga tcaaaggtga tgaacagtac
     2641 ccgaaagacc agatcaagct gtgcgatgat gaactgaaca agttgctcgg ccaggaccag
     2701 gcgtacaagc tggctgtcgc aaaaggtgat tccgcatttg ctatgaatga ttacgaaacg
     2761 gcgcagacgg cgtacacgaa tgcgaaaaac ctcaagccga aagaagccta tcccccggcg
     2821 cagctcgcga aaattgccgg tcttatcgcc gacatgcaga aggataaatc ctaccacgat
     2881 ctgctcgcga aaggagacag cctcttcaac gcaaaagatt acgacggcgc gaaaaagatc
     2941 tataccaccg ctacaacgca gaagccgtcg gatcagtatc cgaaagacaa gctcgccgag
     3001 atcaagcgca tccaggatga tatcgctttc gagcagaaga aagatgcgaa atacaacaag
     3061 ctcatcgccg acgccgacaa gaaattcaaa gccggtgact ggaaaggcgc gcgtgacctg
     3121 tattcgcagg cgctgctcga aaaatcagag aaatatccga gagaccagat cgctgcctgc
     3181 gacaaaaagc tgaatccgcc tccggcaaat acgaaggatt cggtgaaggt gcagacgcat
     3241 gatgaatacg tgaatgatct cgtcacgaaa tatccgcagg gtgtgacgaa aatggaaaac
     3301 caggacggcg gtaccacaac ctatacgacg atcgttgtca tcggcaacca ggcgtgggtg
     3361 tatacgaaga aggtttattc ctacggcacg tattatttca aggatggtga acagatatcg
     3421 gaagcgacat tcaactacga caccaatccc agctacgttg ataaacggct gaacgatctc
     3481 aacaacaacc agaaataggt tgcccggttg cagcttcccg gtgaagtttc attggtgcgg
     3541 aagagaagag gggacaggcg aaaggaaaaa cgccccgtaa agaagaacat cagcggaata
     3601 ccacgaacat cttccggtaa ttgatcacca cctcgttcca ttgcatcagg atatcttcgc
     3661 ctgcaattcc ggagaacatt ccaaaagctg ccgtattgcc gtcgacctgc acggttacca
     3721 tcttcagcgt tccgcttttt cccccgcctt cgtacgcgac gtttttgcaa cggataatat
     3781 gtactttctg cacaccgccg gcaccgccga tgcgtgcggt tttatccttg ccgtgtcctg
     3841 tggtgtaaag cgcatccgag aacttgtgaa gcgcaacagg accgaaaacg gaagtggctg
     3901 cacccgtgtc gaaaataaaa ggcgagtggt cattgaaaaa agaaaggtcg atgaaggaag
     3961 tattcccgta aaaaccgaga ttgatgcccg ggccttcttc ctgcgcgacc gggaaaacga
     4021 tcttcttccg gaagatgtgc agctcgccga gcgcagccag tacaggaaga ccgatgatgc
     4081 cgttgatctt gtaaagtccg ccggcgaagc gaagggaatt atccggcaga acgatgaaca
     4141 ccacattcct gaatttcgca ttgccgatca ccacttcttt cgccacgccc atgcgcgcct
     4201 gcacactgct gcgtgagctc gatgtgatgc tgaaatttcc ggaacgcaca tcgagtccca
     4261 tttcctgcgc ctgtgtttcg gtgatcgcgg aaaaattggc gccggtatcc atcacgtaat
     4321 cgttcgcatg cccgttgatc tgcacaggta tgcggatgag attggttatg tcccttttgt
     4381 aatcgagact gaaatccccg ttccgctcaa cggtcatcgg gtcgatgtct ttcagcagcg
     4441 cggaaaggtc tcccgcttcg cgcacgcctt tcttctcatc gtcatccatg atgcttccgt
     4501 aacggaacag cagatgcgtg cagatggaat ccgcttccct gaagcgggaa agacgaagct
     4561 tgtcctggaa cacaagcatg agcgcatgtt ccgccaggga atcgggacag ttcatggctg
     4621 tcgagtctgt cagctgccgc gtgatcgctt cggaaatttc cgggcgattg aaaagtgaat
     4681 tgttccagac ggaaaagtaa ttcctgtaca gcggatcgag gttcaccgaa tcggcgagga
     4741 attcttcgcg gaaatggaaa tagtcgcctg tgttgacgag attaaataat ttttccgtgt
     4801 gtgcttcgcc gttgctcgcg gaaagcggga cggacagcag cgcaggcagc agggtgaaaa
     4861 aaaacttttt cataaatggt taatccagga acactttgtc gctgccgttc tttccaaact
     4921 ccacggtaat gtgctcctgc atcgtggtcg aaagcgaaag accgacgaaa ttggttttga
     4981 ccgggaagcg attgtgatcg cggtcgacaa ggaccacggt gagaatggat tttatcttaa
     5041 agcggagaaa gaaatcgacg ccatacatca gcgtgcgccc ggaattggaa acgtcgtcga
     5101 cgacaatgat cgtttttccg ctgaagtctt tttcgggcag gctgaccgat gcgctttttt
     5161 cgagcgggtt ttccttgtcc actttcagcg cggcgacagt aaccttcagc ggactgatgc
     5221 tgcgcaggac agcagcgatc ctttccgaaa gcgtaagccc gttcggctcg atgcccgcaa
     5281 tgatgatctc tttttcattg aagcaatgtt cgtaaacctg ccaggcaaga cggttgatgc
     5341 gtcgttctat ctgccctgca ttcattacca gtgttcgttc gctgctcatg gaatattttt
     5401 cttccgcaaa attgataaaa aaacaaagcc cgccggaaat tttcggcggg ccgtcaggat
     5461 aaaatgcgtt accgtacgag ggaaacgtgc ccgatgaact tgtgcttttt gccgaagaca
     5521 tccacgacgg ttgccttcca cacatacaca tcttcctggc agatatttcc gctctttccc
     5581 tgcacggttc cgtcccaggg cttcgacata tcgctggtgt agtagatcat atttccccag
     5641 cggtcgaaca cccacatttc gtatttgtct attccctggc ccacaccgat gaagccgtca
     5701 ttgatgccgt caccgttcgg ggtgaacgcg ttcgggaaat agatggaata ttcaggatcg
     5761 atgcagacgc gcatggtgtc cgttccggga cagttgtcaa ggttgttggc gacgaggatg
     5821 acatcgtagc aacccggctg gtcattgggg aaatcatacg caggattctg cacgttcgag
     5881 ctgtcgagcg taccgaaata ccatgtccat gaaacaggat taccggtcga ttcatccgtg
     5941 aatgtgatgt tggattcgaa cacggtcgtc ggctgcggtg ttgcagtgaa ttgagcaacg
     6001 acattttcgc tcacgcagat gtaggaaggc atcgtgacgg ttcccgtaca tccgttggcg
     6061 tcattgacgg tcagcgttac atcataacaa ccgggatttg catagcagtt ggacgggttg
     6121 cagttgcccg agccaaggtt cccgtcaccg aaatcccagc tgcagttcac ccaggaagga
     6181 ttgttcggtg tcatgaagtt cacgcagagc ggggcacagc ctgtaagcgg atcaccggta
     6241 aaggacggag caggtggcgg atttacagtg acggttgtga acgcagaatc atccggcgag
     6301 caaccgtctg aaataattac cgtgtacgtt gttgtggttg cgggtgtgac gtttgcggtc
     6361 gtcgtgttgc ctgccgtacc agccggcagc caggtgtagg tgtaaggtcc gccgttgccg
     6421 ccgccgcccg ctgcggtgag tgttgacgac gcgcccgcac agacagctgc cggtgcggaa
     6481 ttgagattga tgttcagcgg aggatcaaca gtaaccgtta ccgtggccgg agctgaagcg
     6541 cacccgttcc cgtcagcgac agtaacggta tagccctgcg ttgttcccgg tgaaactgta
     6601 acgctttgcg tcgcgcctgt tcctgcagga gcccagttgt acgtgtatcc tgcgcccgtt
     6661 ccgcccccgg gcgtgcaggt aagcgtggaa gattgcccga tgcagatcgt agacggagcg
     6721 gcagcgacca cagcagtaac gggcggcgga gaagttacct gcacggtggc tgaacccgaa
     6781 cagttgttgg cgtcgctgta attgaccgtg taggttccgg cgcatagatt cgttgcggtt
     6841 gccgttacct ggttcgcggc attcgcatcc cactggtaat tgtagggagc tgttcctcca
     6901 ttgagcgtaa ccgtcgctgt tccgtcgcat acgccgttgc aggaagtagg cgtggaagta
     6961 atgtttgccg caaggtttcc gggaacgccc gttaccacga cggaaatatt cgccaggcag
     7021 ttattggcat ctgtaacgag aacgttgtaa gtaccggcgg gaagattgct cgctgtattt
     7081 gaaccgggca ggttcgttgc ggtctggatg acattattcg cggaatccgt ccacacatag
     7141 tcataaggac ccgtaccgcc tgcaggtgta accgttccgc ttccgttgct ctggccacag
     7201 ctggccggtg tggatgcgac attcagcaca agctgggtcg gctgggtaat gacaatgttg
     7261 gttgtggtaa cacagttgtt gctgtgatcc gtgacagtga cgtgatacgt tcctgcggaa
     7321 aggttgttaa gcacatccgg accattctgg ttgagcgttg tctggatcgt gttgttcgaa
     7381 ttatccgtcc agacataatc gaaaggaccg gttcccaatg cagttgcggt gatggatccg
     7441 ttgttcccgc cgaaacaggt tacgttggtt tgtgcgatct gcggaggtgc gcagcaggcg
     7501 agcgaggcgt tgaactggta gattggatcg ttggaacagg cgacattcgt ccagttaccg
     7561 gtttccccgt cgccgtacgt gtcgatgttg atgctgaggt catcaccgtt ctgcccggga
     7621 ggacatgcat tcgcggtgat cgtccagcag aaggtccagt tcacgtttcc ggtgcagtta
     7681 tcaccgaagt tattcgtggg atcgccatcg ggaccgccct gtacgatgtc tccgtcgaag
     7741 aaaaatccgg attgcgttcc gttcccgttc gggaggttca cgttattgaa ccattgccat
     7801 acgcccggac cgccgtcaca cgt
//
LOCUS       DASTSF010000063         5357 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_442625,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000063 DASTSF010000000
VERSION     DASTSF010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5357
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_442625"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(103..1194)
                     /locus_tag="VFU15_01895"
     CDS             complement(103..1194)
                     /locus_tag="VFU15_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic/DOC family N-terminal domain-containing
                     protein"
                     /protein_id="HEU4716549.1"
                     /translation="MEPYIPDSLPLANLEWSELVEYISVANQAIARYDGLLQSIPNPM
                     ILLAPLQTKEAVMSSRIEGTQATLEEVFAYEAGEAESGDKQHDIMEVINYRIAMNFAI
                     REMQDRPINLNMLRRMHEILLQGVRGENKSGCDFRRIQNWIGQPGSTRENARFVPPSP
                     ANLMHYLGEWEKYIHHDDKDRLVQLGIVHAQFEIIHPFLDGNGRMGRILIPLFLYHHG
                     IIHRPAFYLSAYFEKYRNEYYDHLKSITDTRNWTAWLKFFLKAVIEQSKENTEKVKLI
                     LGLYNEMKEKISEKTHSQFAIQTLDQLFITPVFNSPGFLKETKIPKPSANRILVTLAR
                     NEIIEITRKGKGRRPTVYRFRRLLDIVNH"
     gene            complement(1334..2434)
                     /locus_tag="VFU15_01900"
     CDS             complement(1334..2434)
                     /locus_tag="VFU15_01900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716550.1"
                     /translation="MKTSPFQHGYLSAEELEKFLSGKMTDAQRSAAQKLLDDCELSKE
                     AVEGFSAVPAALADIPSLHAKIGAAAGMKTGALVLKSIIAVTAIAVVAAVTYFAWPEN
                     KSQPPAPVTTVSAPAQENVPAENVLSPSTDNFVNPEAKTVFAPVVITAKAGKDSASPV
                     TEKPEEMVMLPSVPVTDSVVAVKVVPAKPETGYNAEVGFVLDLKVTDFSKLYNGVIEE
                     KELQLTGVPAQFDDAGERDAEKKDEPAVRKVPAETYLRDGLAAFRDGHYGRCIEKMDV
                     LRSNNPEDLNAVFYTGVSYVKLEMYSKAVPLFDKILASTNNVFHEEAKWYKAQALEGE
                     GDVAGAKQLYSEIVSAGGFYAAKAQEKLKGLK"
     gene            complement(2431..3000)
                     /locus_tag="VFU15_01905"
     CDS             complement(2431..3000)
                     /locus_tag="VFU15_01905"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU4716551.1"
                     /translation="MRFFPRKWKSNLSDEELLKEYRSSGDKAFVGELYIRYSHLVYGL
                     CLNYFRDRELARDAVLQLFEKLMTVLTKNEPENFKMWLTFVARNHCISELRKIQVQRD
                     RKQEYASAPGEDNTDREELLKKEERLSRLEEAVKLLGEEQRKCIELFYFSEKSYEEIS
                     EITGYSEKQVKSYLQNGKRNLKIIMEENQ"
     gene            complement(3137..4165)
                     /locus_tag="VFU15_01910"
     CDS             complement(3137..4165)
                     /locus_tag="VFU15_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012465183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HEU4716552.1"
                     /translation="MTTTTENIKLTSKDFVTDQEVRWCPGCGDYSILKQVQTVMPDIG
                     VARENIVFISGIGCSSRFPYYMETYGMHSIHGRATAIASGLKATRPELSVWIITGDGD
                     SLSIGGNHLIHLLRRNLDVNILLFNNEIYGLTKGQYSPTSHEGQVTKSSPMGVIDHPF
                     NPLALAMGASGTFIARTMDRDPAHLRSLLTRANAHKGTSLVEIYQNCNVFNDGAFEIF
                     TEKGTKKAEALFLEQGKPLVFGENGEKGIRLDGLTPRIVDTKDFAADDLWIHDEHDEG
                     KATLLVRFFDDVHKPGHLPRPFGVFYEGKRPCYEELMAEQITHAIAEKGEGDLDKLLR
                     GKNTWEVR"
     gene            complement(4226..>5357)
                     /locus_tag="VFU15_01915"
     CDS             complement(4226..>5357)
                     /locus_tag="VFU15_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxoglutarate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HEU4716553.1"
                     /translation="NQALSYGLIAAGQRSGLPIFLGAYPITPASDILHELAKHKNFGV
                     KTFQAEDEIAGISSAIGASFGGNLGVTASSGPGIALKTEAIGLAVMLELPLVICNIQR
                     GGPSTGLPTKTEQADLLQALYGRNGECPVAVVAAHRPNDCFETVYEACRIAVEHMTPV
                     IFLSDGYIANGAEPWQFPEAKDLVPVRTRFAKNELKEGEKFLPYKRDEKLVRAWAVPG
                     TPGLEHRVGGIEKEHETGNISYDPVNHEFMVKMRQAKIDRIADFIPELKTEFGPEKGK
                     VLVLGWGSTYGVIRTACLELQEEGVEISHAHLRYLNPFPKNLGALLSGFEKVLIPEMN
                     NGQLVKVIRDKFLVDAIAFNKIKGIPFTTAELKEKIKELRNK"
BASE COUNT         1160 a         1435 c         1353 g         1409 t
ORIGIN      
        1 tgagcgtaac gacggaaagt cggaggaaaa tgcgttctgt cttgccggaa ttcattcggc
       61 acaaaggtag cggttgggaa tgatccggtg catgctcccg cattaatgat tgacaatatc
      121 cagcaagcgg cggaagcgat aaacagtagg tcttctcccc tttccttttc ttgttatttc
      181 aataatttca ttccgcgcga gcgtgacaag aattctgttt gcgctcggct tgggaatttt
      241 cgtttctttc aaaaagccag gtgaattgaa aacaggagtg atgaacaact ggtcaagcgt
      301 ctgaatagca aactgcgaat gtgttttttc tgatattttc tctttcattt cattatacag
      361 tccgagtatc agctttactt tttccgtatt ctcttttgat tgctctataa cggctttaag
      421 aaaaaacttt agccacgcag tccagttcct tgtatccgta atgcttttca aatgatcata
      481 atactcattt ctgtattttt caaaataggc actcaggtaa aaagcgggac gatgtataat
      541 tccatggtgg taaagaaaaa ggggaatcag tatcctgccc atgcgaccat tcccgtcaag
      601 aaagggatgt atgatttcga actgggcgtg gacgatacct aactgtacga gccggtcctt
      661 atcatcatgg tgaatatact tttcccattc acccaggtag tgcatcaggt ttgcgggaga
      721 aggtggaaca aaacgggcgt tttcccgggt gctgcccggc tggccaatcc agttctgaat
      781 tctccggaaa tcacacccgg atttattttc tcccctgacg ccctggagca gtatctcatg
      841 cattcttctc aacatgttaa gattgatggg gcgatcctgc atttccctga tggcaaaatt
      901 catggctata cggtaattaa tcacctccat tatatcgtgc tgcttgtctc ccgattctgc
      961 ctctcccgct tcgtatgcaa aaacttcttc cagtgtagcc tgtgttccct ctatcctgga
     1021 ggacataacg gcttcttttg tctggagggg cgcaagcaat atcatgggat tcggtattga
     1081 ctgcaaaaga ccatcgtaac gggctatcgc ctggttcgcc acagaaatgt attcgaccag
     1141 ttcagaccat tcaaggttgg caaggggtaa actatcaggg atataaggct ccattttatg
     1201 acttatcaaa gagccacaaa gttattatcg tctcatatat catcaaaaat gagacataat
     1261 ttaattaaca gtcagtctgc gatacacata aaacactatg aatcaataca taatgttttc
     1321 cgggtaatgc ctgctacttc aaccccttca gtttctcctg ggcttttgcg gcgtaaaatc
     1381 cgcctgctga aacaatttcg gaatacagct gtttcgcacc ggcgacatcg ccttcacctt
     1441 cgagcgcctg ggctttgtac catttcgctt cttcgtgaaa aacattattc gtggaagcga
     1501 ggatcttatc gaacagcgga acggctttgc tgtacatttc cagcttcacg tagctgacgc
     1561 ccgtataaaa aaccgcgttg aggtcttcgg ggttattgct ccgcagcacg tccatttttt
     1621 cgatgcagcg cccgtaatgc ccgtcgcgga aagcggcaag cccgtcgcgc aggtaggttt
     1681 ccgccggaac tttcctcacg gcaggttcat ccttcttttc tgcatcgcgt tcgccggcat
     1741 catcgaactg cgcaggcact cctgtcagct gtaattcctt ttcctcgatg acgccattgt
     1801 acagtttgct gaaatccgtc actttgagat cgagcacaaa accgacttcg gcattgtaac
     1861 cggtttccgg cttcgcggga acgactttca ccgccaccac cgaatcggtt acgggaacag
     1921 acgggagcat aaccatctct tccggttttt ccgtaacggg cgaagcggaa tctttcccgg
     1981 ccttcgcggt aatgaccacg ggagcaaaaa cagttttcgc ttcgggattg acgaaattat
     2041 ccgtcgaagg ggaaagcacg ttttccgcgg gaacattttc ctgcgcggga gcagaaactg
     2101 tcgttacggg tgcaggcggc tgcgatttat tttccggcca cgcgaaatac gtgacagcgg
     2161 caacgaccgc aatggcggta acggcgataa tggacttgag gaccaaagcg cctgtcttca
     2221 tcccggcggc cgccccgatc ttcgcgtgaa gggaaggaat atccgccagc gcagcaggca
     2281 ctgcagagaa tccttccacg gcttctttcg aaagctcgca atcgtccagc agcttctgtg
     2341 ccgccgaacg ctgcgcatcc gtcattttcc cggaaagaaa tttttcaagt tcttccgcgg
     2401 aaagataacc gtgctgaaaa ggggatgttt tcattggttt tcctccatga tgattttcag
     2461 gttccttttc ccgttctgca ggtagctttt cacctgcttt tccgaataac ctgttatttc
     2521 tgaaatttcc tcgtatgatt tttcactgaa gtaaaacagc tcgatgcatt tgcgctgctc
     2581 ttctcccagc agcttcaccg cctcttccag ccgcgacagg cgctcttctt tcttcagcag
     2641 ctcttcccgg tccgtgttgt cttcaccggg cgccgatgca tattcctgct tcctgtcccg
     2701 ctgcacctgg atcttccgca gctcggagat gcaatgattg cgtgcgacga aggtcagcca
     2761 cattttgaaa ttctccggct cgtttttcgt gagcaccgtc atgagctttt cgaacagctg
     2821 cagcacggcg tcacgcgcca gctcccggtc acggaaatag ttcaggcaca aaccgtagac
     2881 gagatgagaa taacggatgt acagctcgcc cacgaacgct ttatcgccgg aggaacggta
     2941 ttccttcagc aattcctcat cgctgaggtt gcttttccat ttgcggggaa aaaatcgcat
     3001 agccttcagg ttatagacgc cggaaagtta catttccata taaaacgggg atggattcgc
     3061 agcgcagggc gaaatctcca ttcctcaaga atgctgccgg tcagaatggt atacgcagac
     3121 agccggcaag ggttgctcag cgcacttccc aggtgttctt cccgcgcagc aatttatcga
     3181 ggtcgccttc tcctttttcc gcaatggcat gtgtgatctg ttcggccata agctcctcgt
     3241 agcacgggcg ttttccttcg taaaatacac cgaacgggcg cggcaggtga ccgggtttgt
     3301 gaacgtcgtc gaagaaccgc acgagcaacg tcgcttttcc ttcgtcatgc tcatcgtgga
     3361 tccagagatc atccgccgca aaatcctttg tatccacgat gcgcggcgta agcccgtcga
     3421 ggcggatacc tttttccccg ttctcaccga acacgagcgg ttttccctgt tccaggaaaa
     3481 gcgcttccgc ttttttcgtt cctttttcgg tgaagatctc gaatgccccg tcgttgaaaa
     3541 cgttgcaatt ctggtagatc tcgaccagcg atgttccttt gtgcgcattc gcccgcgtca
     3601 gcagggaacg caggtgcgcg ggatcacggt ccattgtgcg cgcgatgaac gttccggatg
     3661 cgcccatggc aagcgcgagc gggttgaacg ggtgatcgat cacacccatg ggtgacgatt
     3721 tcgtgacctg tccttcgtgc gaggtgggag aatattgccc tttcgtcagc ccgtagatct
     3781 cgttgttgaa caaaaggata ttcacatcga ggttccggcg cagcaaatga atgaggtggt
     3841 ttccgccgat cgaaagcgaa tcgccgtcgc cggtgatgat ccatacactc agttccgggc
     3901 gcgtggcttt cagtccgctg gcaatggcgg tggcgcggcc gtgaatggag tgcatgccgt
     3961 aggtttccat gtaatacggg aaccgcgacg agcagccgat gccggaaatg aagacgatgt
     4021 tttccctcgc cacgccgata tcgggcatga cggtctgcac ctgcttgagg atggaataat
     4081 ctccgcagcc cgggcaccag cgcacttcct ggtcggtgac gaaatctttg gaggtgagtt
     4141 ttatgttttc tgttgttgta gtcatgtctg gtagccgctg aggcgcgaag acgcagggaa
     4201 tccggcgcgg ttttgttgtt tgaaattatt tattccgaag ttcttttatt ttctctttca
     4261 gttccgctgt cgtgaacggt atccctttga tcttgttgaa cgcgatcgcg tcgacgagaa
     4321 atttatcgcg gatcactttc acgagctgac cgttattcat ttcgggaatg agcacttttt
     4381 caaaaccgct caaaagtgcg ccgaggtttt tcgggaacgg gttgaggtaa cgcaggtgcg
     4441 cgtgcgaaat ttcaactcct tcttcctgca gttcgaggca agccgtgcgg atcacgccgt
     4501 acgtggagcc ccagccgaga acaagcactt tccctttttc cggtccgaat tctgttttca
     4561 gctccgggat aaaatcggcg atgcgatcga tcttcgcctg gcgcatcttc accatgaact
     4621 cgtggttcac cgggtcgtag ctgatgttgc cggtttcatg ctctttttca attccgccca
     4681 cacgatgttc gagtccgggc gttccgggaa cagcccaggc gcgcacaagt ttttcatcgc
     4741 gtttgtacgg gagaaatttt tcgccctcct tcagttcgtt ctttgcaaat cgcgttctca
     4801 ccggaacaag gtctttcgct tccgggaact gccacggttc cgcgccgttg gcgatgtagc
     4861 cgtcgctgag aaagatcacc ggcgtcatgt gctccaccgc gatgcggcag gcttcataca
     4921 ccgtttcaaa acaatcgttc gggcggtgcg ccgcaacaac ggcaacggga cattctccgt
     4981 tccttccgta taacgcctgc agcagatccg cctgctcggt tttggtcggc agtcccgtac
     5041 ttggtccgcc acgctggatg ttgcagatga cgagcggaag ctccagcatc acggccagcc
     5101 cgatcgcttc tgttttcagc gcaatgcccg gacccgacga agccgttaca ccgagatttc
     5161 caccgaacga ggcgccgatc gccgaggaaa tgccggcgat ctcatcctcg gcctggaatg
     5221 ttttcacgcc gaaattctta tgcttcgcca gctcgtgcag gatgtccgac gccggcgtga
     5281 tcgggtacgc gccgaggaag atcggcagcc cggagcgctg ccccgccgcg atcaggccgt
     5341 aggacaacgc ctggttg
//
LOCUS       DASTSF010000064         5029 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1104618,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000064 DASTSF010000000
VERSION     DASTSF010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5029
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1104618"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            98..1138
                     /gene="lpxD"
                     /locus_tag="VFU15_01920"
     CDS             98..1138
                     /gene="lpxD"
                     /locus_tag="VFU15_01920"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013552858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HEU4716554.1"
                     /translation="MNFSAKDIAALAGGTVEGNPDITVSKLAKIEEGEAGALSFLSNP
                     QYEQFIYASKASIVIVNSSFTPEKELPEGMTLVRVGDARQAFAKLLEAYNGFRLNKTG
                     IDAGAYVSPSAKIGRNVYVGAFAYVGDHAVISDNAKIFPQVFIGENAKIGTGTLLHSG
                     VRVYHDCVIGNNCIIQSGAIIGGDGFGFQPNSENNYQKVPHIGNVVIHDNVEIGANTT
                     VDRATLGSTIIHKGVKLDNLIQVAHNVEIGENTVIAAQSGIAGSTKIGRDCLIGGQVG
                     IIGHLTIGSRVKIAAQSGIGSNLKDDEIVQGSPAFAIGDYKRSYVIFRSLPSLSDKVK
                     ELSKHINQEKEK"
     gene            1154..2584
                     /locus_tag="VFU15_01925"
     CDS             1154..2584
                     /locus_tag="VFU15_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013664830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase/3-hydroxyacyl-ACP
                     dehydratase"
                     /protein_id="HEU4716555.1"
                     /translation="MSSKQHTIGKPVSVSGVGLHTGKTCNLTFRPAEENHGFKFRRVD
                     LPGQPVIDADVDNVVDTARGTTLEQNGARVYTTEHVLAALVGCEIDNAIIELDAPEVP
                     ILDGSSAPFVAALAEAGRVEQKAEREYFELKENLTYEDPVKKVEMLAVPQDEFRVTVM
                     VDYNSEILGTQHASMYNIGEFSSAIAKCRTFVFLHELEALLQHNLIKGGDLDNAIVLV
                     DRELEKDKMDHLRKVFNKPDVEVKGRGVLNNTSLHFYNEPARHKLLDIVGDLALVGVP
                     MKIHVLAARPGHAGNVEFAKKLKALVKKERSKKQEVNFNVQAPALFDVRDIMKILPHR
                     PPFLFVDKIIELSPTHVIGCKNVTMNEFWTVGHFPDEPIMPGVLLVEAMAQTGGILCL
                     KTVPDPENYQTYFLKIDGVKFKQKVIPGDTCVFKLDLVEPIRRGLCHMKGTTYVNNKV
                     AAEADMLAQIVKVRNIESKVAQPASV"
     gene            2581..3357
                     /gene="lpxA"
                     /locus_tag="VFU15_01930"
     CDS             2581..3357
                     /gene="lpxA"
                     /locus_tag="VFU15_01930"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HEU4716556.1"
                     /translation="MISPLAFIDPNAKIGENVTVEPFAYIQGDVVIGDGTWIGPSAVI
                     MDGARIGKNCRIFSGASISAIPQDLKFDGEKTTTEIGDNTTVREFCTVNRGTKDRYKT
                     VVGSNCLLMAYVHIAHDCVVGNNVILANSVNLAGHVVIEDFVILEGFVGVSQFVHIGA
                     HSFVAGQSGARKNVPPFVKAAREPLAYVGVNSVGLRRRGFSNESILQIEDIYRTLYVK
                     GLNVSNAVGVIEMEAPESKEKEIILEFIRNSKDGIIRGIS"
     gene            3403..3924
                     /locus_tag="VFU15_01935"
     CDS             3403..3924
                     /locus_tag="VFU15_01935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716557.1"
                     /translation="MKKLFLFLFLLPFTSALSAQGSVRTTFNKSYDRWDVKVGGSSGT
                     ISSTFSGNATRWDISMGDVSGTISATFSGSYSRWDVNVGGKNYTLTQTFSDGNRWELS
                     GGDLKSAVVIATTFNNDFSRWDMNGVKISSTFSDSYDSWDISGNTDNLTAGEKAVAAF
                     IPVMVGNGIIKKQ"
     gene            3927..4493
                     /locus_tag="VFU15_01940"
     CDS             3927..4493
                     /locus_tag="VFU15_01940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716558.1"
                     /translation="MKKLLPLPLLFFVLTSFVVLQDAGEQDGRVRTFMTGKIDRWDVT
                     LGDEEGNIEAYMKDDPNRWTFEIDTMSGEVNSEFTDKFNSWEITVGTKTYKLKTWLSA
                     SWTKWELSGGDIIGKESIQTLYANSWDNWEMKQEDSVRADITTYYGNSWDDWNIRADM
                     KKLNEGEKITCVFMPLFVSRIYRKGLAH"
     gene            complement(4805..>5029)
                     /locus_tag="VFU15_01945"
     CDS             complement(4805..>5029)
                     /locus_tag="VFU15_01945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716559.1"
                     /translation="TPSVSQSARRKTKTTRNLKLKTVLTTKDTKRFTKCTKKNKNNPK
                     SETKKCFNHEEHKAFHKVHEEKQKQPETRN"
BASE COUNT         1177 a         1273 c         1406 g         1173 t
ORIGIN      
        1 tatcacgcgg aagaaagcac ttgtgaaggc cggggtgtga acaatttggc tgcggacggc
       61 tccctgctga cctttctttt ttagttaaat ttaccggatg aatttttcgg ccaaagacat
      121 tgcagcgctg gctggcggaa cggttgaggg aaaccccgat atcaccgtat caaagctggc
      181 gaagatcgag gaaggcgagg cgggcgcatt gtcttttctt tcgaatccgc agtacgaaca
      241 atttatttac gcatcgaagg cgagcatcgt catcgtcaac agcagcttta cacccgagaa
      301 agaattgcct gaaggcatga cgctcgtgcg tgtcggtgat gcaaggcagg cgttcgcaaa
      361 gctgctcgaa gcgtataatg gtttccgtct caataaaaca ggcattgatg cgggcgcgta
      421 cgtttcccct tctgcgaaga tcggcaggaa cgtgtacgtc ggcgcatttg cgtacgtggg
      481 cgatcatgcg gtgatcagcg acaatgcaaa aattttcccg caggttttta tcggggaaaa
      541 tgcgaagatc ggaacgggca cgctgctgca ttccggtgtt cgtgtgtatc acgactgcgt
      601 gatcggtaac aattgcatca tccagtcggg cgcgatcatc ggcggagacg gattcggttt
      661 ccagccgaac agcgagaaca attaccagaa ggtgccgcac atcggcaacg ttgtcattca
      721 cgataatgtc gagattggcg cgaacaccac cgtcgaccgc gcgacgctcg gctccacgat
      781 cattcacaaa ggcgtgaagc tcgacaacct catccaggtg gcgcacaacg tcgagatagg
      841 agagaacacc gtcatcgctg cgcaatcagg tattgccggc agcacgaaga tcggccgcga
      901 ctgtctcatc ggcgggcagg tcggcattat cggtcacctc accatcggca gccgtgtgaa
      961 gatcgccgcg cagtccggca tcggcagcaa cctgaaggac gacgagatcg tgcagggttc
     1021 gccggcattc gccatcggcg attacaaacg ttcgtatgtt attttccggt cgctcccttc
     1081 gttaagcgat aaagtgaagg agctttcaaa gcatatcaac caggaaaaag aaaaataaac
     1141 ccgcctcttg aaaatgagca gcaagcaaca taccatcggg aaaccggttt ccgtttccgg
     1201 cgtcggtctg cataccggca aaacctgcaa cctgacgttc cggccggcgg aagaaaatca
     1261 cgggttcaaa tttcgccgcg tggatcttcc cggccagccg gtgatcgatg cggatgtgga
     1321 caacgtggtg gacacggcgc gcggaacgac gctcgagcag aacggtgcgc gtgtgtacac
     1381 gacggagcat gtgctggctg cgcttgtggg ctgcgagatc gacaatgcga tcatcgagct
     1441 ggatgcgccg gaagttccca ttctcgacgg gagctctgcg cctttcgttg cggcgctcgc
     1501 ggaagccggc cgcgtggaac agaaagcaga gcgcgaatat tttgagctga aggaaaatct
     1561 cacctacgaa gatcccgtca aaaaagtgga gatgctggcc gtgccgcagg acgagttccg
     1621 cgtaacggtg atggtggatt acaactccga aattctcggc acgcagcatg cgtcgatgta
     1681 caacatcggc gagttcagct cggccatcgc gaaatgcagg acgttcgttt tcctgcatga
     1741 gctcgaggcg ctgctgcagc ataacctcat caagggcgga gatctcgaca acgcgatcgt
     1801 gctcgtcgac cgtgagctgg aaaaagacaa aatggatcac ctgcgcaagg tgttcaacaa
     1861 gcccgacgtg gaagtgaaag gacgcggcgt gctgaacaac acgagcctgc atttttacaa
     1921 cgaacccgcg cgtcacaagc tgctcgacat tgtcggcgac ctggcgcttg tcggtgttcc
     1981 gatgaagatc cacgtgctgg ccgcacggcc gggtcacgca ggtaacgtgg aattcgcgaa
     2041 gaagctgaag gcgctcgtga aaaaagaacg ttcaaaaaag caggaagtga atttcaacgt
     2101 gcaggcaccg gcgctcttcg acgtgcgcga catcatgaag atacttccgc accgtcctcc
     2161 tttccttttt gtcgataaga tcatcgagct ttcgcccacg catgtcatcg gctgcaaaaa
     2221 tgttacgatg aacgaattct ggaccgtcgg ccattttccc gacgagccca tcatgccggg
     2281 cgtgctgctc gtggaagcga tggcgcagac aggcgggatc ctctgcctca aaaccgttcc
     2341 cgatcccgaa aattaccaga cgtattttct caagatcgac ggtgtgaagt tcaagcagaa
     2401 agtcattccc ggcgacacgt gtgtattcaa gcttgacctc gtcgaaccga tccgccgcgg
     2461 actgtgccac atgaaaggaa ccacctacgt caacaataaa gtggcggcgg aggccgatat
     2521 gcttgcacag atcgtgaaag tccgtaacat tgagtccaaa gttgcacaac ccgcgagtgt
     2581 atgatctctc cgcttgcctt tattgatccc aacgcaaaaa tcggtgaaaa cgtaacggtc
     2641 gaaccgtttg cctacatcca gggcgacgtc gtgatcggtg acggcacgtg gatcggcccg
     2701 agcgccgtga tcatggatgg cgcgcgcatc gggaagaact gcaggatatt ttccggcgct
     2761 tccatcagcg cgatcccgca ggacctgaaa ttcgacggcg aaaaaaccac caccgagatc
     2821 ggcgacaaca cgacggtccg cgaattctgc acggtcaacc gcggtacgaa agaccgttac
     2881 aagacggtgg tcggcagcaa ctgcctgctc atggcatacg tgcacatcgc gcacgactgc
     2941 gtcgtcggca acaatgttat tctcgcgaat tccgtcaacc tcgccggcca cgtggtgatc
     3001 gaagatttcg tcatcctcga agggttcgtc ggtgtttcgc agttcgtgca catcggtgcg
     3061 cacagctttg tggcgggcca gtcgggtgcg cgcaaaaatg ttccgccttt cgtaaaagcc
     3121 gcgcgcgagc cgctggcgta tgtaggtgtg aattcggtcg gccttcgccg ccgcgggttt
     3181 tcgaatgaat ccatcctgca gatcgaggat atttaccgca cgctctacgt gaaagggctc
     3241 aatgtatcga acgccgtagg cgtgatcgaa atggaagcgc ccgaatcgaa agagaaagag
     3301 atcatcctcg aattcatccg caattcgaaa gacggcatca tccgcgggat ctcctgacac
     3361 tgcggcaagg ttcttgcatg aaaaaaggcg taactttctt tcatgaaaaa acttttcctt
     3421 ttcctgttcc ttctcccgtt cacttctgcg cttagcgcgc agggaagcgt acgcaccacg
     3481 ttcaacaaaa gctacgaccg ctgggacgtg aaggtgggcg gatcttcggg aaccatctca
     3541 tccacctttt cgggtaacgc aacgcgctgg gacatttcca tgggcgacgt ttcaggaacg
     3601 atctccgcaa ctttttccgg aagctattcg cgctgggatg tgaacgtcgg cgggaaaaat
     3661 tacacgctta cgcagacctt ttccgacggc aaccggtggg agctgagcgg cggcgatctc
     3721 aaaagcgccg tcgtcattgc caccacgttc aacaatgatt tctcgcgctg ggacatgaac
     3781 ggtgtgaaaa tttcctccac gttttccgac agctacgaca gctgggatat ttccgggaat
     3841 acggataacc tgacggcggg cgaaaaagct gtggctgcat tcatccctgt catggtcggg
     3901 aacgggatca tcaaaaaaca gtaaccatga aaaagctgtt gccccttccg ctgctttttt
     3961 tcgtgctgac gtccttcgtc gttttgcagg atgccggtga acaggacggt cgcgtgcgga
     4021 cgttcatgac cgggaagatc gaccggtggg acgtaacgct cggcgacgaa gagggaaata
     4081 tcgaagcgta catgaaagac gatcccaacc gctggacgtt cgagatcgat acgatgagcg
     4141 gcgaagtgaa ttccgaattc acggacaagt tcaacagctg ggagatcacc gtcggaacca
     4201 aaacctacaa gctcaaaacc tggctcagcg catcgtggac aaaatgggag ctgagcggcg
     4261 gagatatcat cgggaaggaa agcatacaga cactttacgc gaacagctgg gacaactggg
     4321 agatgaagca ggaagacagc gtgcgcgcgg atattactac ttattacggc aacagctggg
     4381 acgactggaa catccgtgcc gacatgaaga agctgaacga gggcgagaag atcacctgcg
     4441 ttttcatgcc gctttttgtg tcgaggattt accgcaaagg actcgcgcat tgaacaggca
     4501 cgtgcgcttt atgaaaacac tttgtgtcct tcgtggttga aacagttttt agtttcggat
     4561 ttcgggtttc aggtttcagg ttgttttctt tgcgcgcctt tgtgaaaaca ctttgtgtcc
     4621 ttcgtggtta aaacagtttt tagtttcgga tttcgggttt caggttgttt ttttttgagc
     4681 gccttgtgaa acgctttgtg tccttcgtgg ttaaaacagt ttttagtttc agatttcggg
     4741 ttgattttgt ttttcttcgt gttctttgtg aaaagcttgg tgttcttcgt ggttgaaaca
     4801 ctttttagtt tcgggtttcg ggttgttttt gtttttcttc gtgcactttg tgaaacgctt
     4861 tgtgttcttc gtggttgaaa cactttttag tttcggattt cgggttgttt ttgtttttct
     4921 tcgtgcactt tgtgaaacgc tttgtgtcct tcgtggttaa aacagttttt agtttcaggt
     4981 ttcgggttgt ttttgttttt cttcgtgcac tttgtgaaac gcttggtgt
//
LOCUS       DASTSF010000065         5129 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1299245,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000065 DASTSF010000000
VERSION     DASTSF010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5129
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1299245"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1110
                     /locus_tag="VFU15_01950"
     CDS             <1..1110
                     /locus_tag="VFU15_01950"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEU4716560.1"
                     /translation="QTFNLNASGSGTLTWYDQPSGGTALGTGASFTTPVVNSTTTFYV
                     ANEVSQPPGYVGPASYNFGTGGMHNNTSTQYLEFTVYQPCTLVAGDVNSGGTGTRTFT
                     LWDSNGNQISQYPVNVTSGVQTVSLNIPLQPGSYRIGGTQMNLYRNNSGPAYPYNYNG
                     IVSITGSSAGSGYYYYLYNWQIQPQPCASPRTPVVAYIGIPNMALNLPMDTVCQVDGP
                     VTLSGGSPAGGTYSGTGVSNGVFDPTVSGTGTFTITYTYNDTVNGCSGSTTSVIDVET
                     CAGISSAASGVTMAVFPNPATTALTVDLSFGQTEDFVEVSVVNTLGQQMYSTLLNNPG
                     SSARVDINTEDWARGMYLLKVRTSSGTQVRKIETR"
     gene            complement(1189..1602)
                     /locus_tag="VFU15_01955"
     CDS             complement(1189..1602)
                     /locus_tag="VFU15_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004916385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA/BrxB family bacilliredoxin"
                     /protein_id="HEU4716561.1"
                     /translation="MYPPQLVMPMKAELVDVGFEDLTTPDEVDAAIKSPGTVLVVVNS
                     VCGCAAGAARPGVKLSIQGEGKKPNRLATVFAGFDTEATVQARKYFAPYPPSSPSIAL
                     FKDGKLIHFVERHHIEGRSAQMIADHLKMAYDEFC"
     gene            complement(1681..2283)
                     /locus_tag="VFU15_01960"
     CDS             complement(1681..2283)
                     /locus_tag="VFU15_01960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716562.1"
                     /translation="MDRQLMFDYNEFAAIGEAAGTTWLQLPPLETRPLDFTNPLLFIA
                     GAEWNFRYPLREDDRRNYSVCATVPLQLAIVSSDGPSLSVPVNSTSIWLSVPCVITAN
                     MGLGSTPESGDKFLGTYIGAGMALNKAFIREKSYVFTGVPNAPVNYHYRFPIFPTVNA
                     TAGLRFRGLRGYVRNIGVEGSAGFDGIWTIGIVYGTVSGL"
     gene            2452..>5129
                     /locus_tag="VFU15_01965"
     CDS             2452..>5129
                     /locus_tag="VFU15_01965"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEU4716563.1"
                     /translation="MKKIFAAFVALLPFSFVAAQPTSGNQAKAKLVEKVSTNGIDKLV
                     IPYQKYVLPNGLTLVVHEDHSDPLVYVDVTYHVGSAREQEGRSGFAHFFEHMMFQGSG
                     HVADEEHFRLINGAGGTLNGSTNTDRTNYFETVPSNYLEMALWLEADRMGFLLDSVTQ
                     QKFEVQRSTVKNERGQRYDNAPYGLVWEKIGEAMYPEGHPYSWSTIGYIEDLNRVNVG
                     DLRNFFLRWYGPNNATLTIAGDVNTADVVAMVEKYFGTIPRGPEVKNMPHMPVSLSSD
                     RCISYEDNVRFPQLTLAFPGVATWDADEAPLDALCSILSDGKSSVFYDVFIKSQVASV
                     ANMYSATEELSGFIAMNVRCYPGHTPREADSLIGVALNRFESRGVTDDDLTRYKLTVE
                     SYFLDQMSSCRGKGGMLASYQTFRGNPNMIAADLDRYQKVTKEDVMRVFEKYVKGRHQ
                     VELTVYPKGHPEMKARPDNYVIPPHKAGAEESAEYKNLSPRRPVDNFDRNIHPVPGPT
                     PAVKVPQLWRDSFPNGLRAIGTQSLEVPRTYLLLTISMGHRQEDPAKAGVAYMLSKML
                     DQSTKNYSAADMEKQTELLGSDISISLNNDEMQVGVTSFTRNIDATLALVNEKLFSPA
                     FRKEEFDLVKKQQLEDIRNMATRPTAMADFVFDRMIYGADNIMGIPVMGTEATVSALT
                     LDDVIAYYNSMFSPNIARLIAVSDLAQKQLMGKFGFLQAWQNKNVQLKTDANLLPKGQ
                     PGPTKIFLFNKSDAAQSEIRVGRLAMPYDYSGEFFRASMMNYELGGNFNSHLNLTLRE
                     DKAWTYGARSGFSGSSYTGTFTASAGVRADASDSSVYEFMRIIQDYATNGIDTAELSY
                     TKRAVCNGEALRYEDPRQKLIFLKRIIDYNLPVDYS"
BASE COUNT         1222 a         1384 c         1453 g         1070 t
ORIGIN      
        1 caaacgttca acctgaacgc aagcggatcc ggcacgctta cctggtatga tcagccatcg
       61 ggcggaaccg cgctcggaac cggcgcttca ttcacaacgc cggtggtcaa ttcaacaacg
      121 acgttttacg tggcgaacga agtgtcgcag ccgccgggat atgtcgggcc cgcctcgtac
      181 aatttcggaa cgggcggcat gcacaacaac acgtcgacgc aatacctcga gttcacggtt
      241 taccagccgt gcacgctggt ggccggcgac gtcaactccg gcggaacagg aacgcgcaca
      301 tttacactgt gggacagcaa cgggaaccag atcagccagt atcccgtgaa cgtaacatcc
      361 ggtgtgcaga cggtgtcgct caatatcccg cttcagccgg gcagctatcg catcggcgga
      421 acgcagatga atctttaccg caacaattca ggtccggcat acccgtacaa ttacaacgga
      481 atcgtcagca ttacgggctc gtcagcgggt tcaggttatt actattacct gtacaactgg
      541 cagatccagc cgcagccctg cgcaagtccg cgcacgccgg tggtggctta catcggaatt
      601 ccgaacatgg cgctcaatct tcccatggat acggtttgcc aggtggatgg cccggtaacg
      661 ctcagcggcg gatcgcctgc aggcggaacg tattccggta cgggtgtcag caacggggtt
      721 ttcgatccca ccgtttccgg cacaggaacg ttcacgatca cgtacaccta caacgatacg
      781 gtgaacggat gttccggctc caccacatcg gtgatcgacg tggaaacgtg cgcaggaatt
      841 tcttccgcag cttccggcgt aacgatggcc gtgttcccga atcccgcgac gacagcgctg
      901 acggtcgatc tttctttcgg gcagaccgag gacttcgtgg aagtttccgt ggtgaacacg
      961 ctcgggcagc agatgtattc gacattgctg aataatcccg gctcatctgc gcgtgtcgac
     1021 atcaacacgg aagactgggc acgcggaatg tatttgctga aagtgaggac atcttcgggc
     1081 acgcaggtgc ggaagatcga aacgagataa aagcaaaaca gcttcagcgg aaaacaaaaa
     1141 tccccggaga gtatttcttc cggggatttt tcattctgaa aaaatttctc agcagaactc
     1201 gtcgtaggcc attttcaggt gatcggcgat catctgcgcg ctgcgtcctt caatgtgatg
     1261 acgttctacg aaatgaatga gcttgccgtc tttgaaaagc gcgatggaag gagaagaggg
     1321 cgggtaaggg gcaaagtatt tgcgcgcctg caccgttgct tccgtatcga aaccggcgaa
     1381 aacagtagcg agcctgttcg gtttttttcc ttccccctgg atcgacagtt tcacgcccgg
     1441 gcgcgcagct cctgctgcac agccgcacac ggaattgacc acgacgagaa cggttcccgg
     1501 cgatttgatc gctgcatcga cctcatcggg agtggtgagg tcttcgaaac ccacgtccac
     1561 cagctcggct ttcatgggca tcactaattg cggaggatac attgtctgaa tttttggttg
     1621 aacttattaa ttgtggatac aaagatacag gtaaaaaaaa ggaattgtcc gccgcacgac
     1681 ttaaagccct gatacagtgc cataaacgat tccgatggtc cagatgccgt cgaagcctgc
     1741 cgagccttcc acaccaatgt tgcgcacata gccgcgcaat ccgcggaagc gcagcccggc
     1801 cgttgcgttc accgtgggga agatcgggaa acggtaatgg taattcacgg gcgcattggg
     1861 aacacccgtg aagacgtagc ttttttcacg gatgaacgct ttgttcagcg ccatgcccgc
     1921 accgatgtat gtgccgagga atttatcgcc ggattcaggc gtggagccga gtcccatgtt
     1981 ggccgtgatg acgcagggaa cggaaagcca gatgctggtg ctgttgaccg gaaccgagag
     2041 cgacgggccg tcgctggata caatggcgag ctggagcgga acggtggcgc agacagaata
     2101 atttcgccgg tcgtcttcac gcagcgggta acggaaattc cattcggccc cggcaatgaa
     2161 aagaagcggg ttggtgaaat cgaggggacg cgtttccagc gggggaagct gcagccacgt
     2221 tgttccggcc gcctcgccga tcgccgcgaa ctcgttgtaa tcgaacatca gctggcgatc
     2281 catcgcggaa gaatgcgatg cgggagttgt tgaaggaggc gccggagtct gcgcacgaac
     2341 acaaaagggg acgcacaata agaagatcca cgttaatttc cgcatgcttt ttccgtattg
     2401 gcttttctcc ctgaaaattg cgattatttt tgtaagaaca gaacataaaa tatgaaaaaa
     2461 atattcgccg catttgtcgc cctcctcccg ttttcgtttg ttgccgcaca acccacttcc
     2521 ggcaaccagg ccaaggcgaa gctcgttgag aaagtcagca cgaacgggat cgacaagctg
     2581 gtcattccct accagaaata tgtgcttccc aacgggctta cgctggtggt gcacgaggac
     2641 cattccgatc cgctggtgta tgtcgacgtc acgtatcatg tcggttcggc gcgggagcag
     2701 gaaggtcgct ccgggttcgc tcattttttc gagcacatga tgttccaggg ttcgggtcat
     2761 gtggcggatg aagaacattt ccgtctcatc aacggggccg gcggaacgct gaacggatcg
     2821 acgaataccg atcgcacgaa ttatttcgaa acggttccct ccaattacct cgaaatggcg
     2881 ctctggctcg aagccgaccg catgggattt cttctcgatt ccgtgacgca gcagaaattc
     2941 gaagtgcagc gcagcacggt gaaaaacgaa agaggccagc gatacgataa cgcaccttat
     3001 ggtcttgtct gggaaaagat cggcgaagcg atgtatcccg aaggtcatcc gtactcgtgg
     3061 tcgacgatcg gttacattga agatctcaac cgtgtgaacg tcggggacct gcggaatttt
     3121 ttccttcgct ggtacggacc caacaacgca acgctgacca ttgccggtga tgtgaacacg
     3181 gccgacgttg tggccatggt ggaaaaatat ttcggcacca ttccccgcgg acctgaagtg
     3241 aagaacatgc cgcacatgcc ggtctcgctt tcatccgacc gctgcatttc ctatgaagac
     3301 aacgtccggt tcccgcagct gacgctcgca ttccccggcg tggctacgtg ggatgccgat
     3361 gaagctccgc tcgatgcgct ctgcagcatc ctgtccgacg gtaaaagctc ggtcttctac
     3421 gatgtgttca tcaaatcgca ggtggcgagc gtggcgaaca tgtacagcgc cacggaggag
     3481 ctgtcgggtt tcattgccat gaatgtgcga tgttacccgg gtcatacgcc acgggaagcg
     3541 gacagcctga tcggcgttgc gctgaaccgt tttgagtccc gcggcgtgac ggacgacgac
     3601 cttacgcgtt acaagctgac ggtggaatcg tatttcctcg accagatgtc gagctgccgc
     3661 ggaaaaggag ggatgcttgc gtcgtaccag acgttccgtg gaaacccgaa catgatcgcc
     3721 gccgacctgg accgttacca gaaagtgacg aaggaagacg tgatgcgtgt tttcgaaaag
     3781 tacgtcaaag gccgtcacca ggtggagctc acggtttatc cgaaaggaca cccggaaatg
     3841 aaagcgcgtc ccgataatta tgttatcccg ccgcacaaag ccggggcgga agaaagcgcg
     3901 gaatacaaaa atctttcgcc gagaaggccc gtggataatt tcgaccgcaa cattcacccg
     3961 gttcccggac ctacaccggc cgtgaaagtt ccgcagctgt ggcgcgacag ttttcccaac
     4021 gggttacgcg cgatcggaac acaatcgctc gaggtgccgc gaacgtacct gttgctgacc
     4081 atttccatgg gacaccgcca ggaagatccc gcgaaagccg gtgtggctta catgctttca
     4141 aaaatgctgg accagtcgac gaaaaattat tcagcagcgg acatggagaa gcagacggag
     4201 ctgctcggca gcgacatcag catttcactc aataacgacg agatgcaggt gggcgtcact
     4261 tcgttcacgc gcaacatcga cgcgacgctt gcactcgtca atgaaaaatt attcagcccc
     4321 gcgttccgca aagaagaatt cgatctcgtg aaaaagcagc agctcgaaga catacggaac
     4381 atggctacgc gtccgacggc catggcggat tttgtattcg accggatgat ttacggagcg
     4441 gacaatatca tgggcattcc ggtgatggga accgaagcaa cggtttccgc gcttacgctg
     4501 gatgacgtga tcgcctatta caacagcatg ttctcaccga acatcgcacg gctgatcgcc
     4561 gtgagcgacc tcgcgcaaaa gcagctgatg gggaaattcg gtttcctgca ggcgtggcag
     4621 aataaaaacg ttcagctgaa aacggatgcg aaccttttgc cgaaaggaca accgggtccg
     4681 acgaagattt tccttttcaa taaatccgat gcggcgcagt cggagatccg cgtgggccgg
     4741 ctggccatgc cgtatgatta cagcggggaa tttttccgcg cctcgatgat gaattacgag
     4801 ctcggcggga atttcaacag tcatctcaac cttacgctgc gcgaagataa agcgtggacg
     4861 tacggcgcgc ggtcgggttt cagcggatcg tcctatacgg gaacattcac ggcttccgcg
     4921 ggtgtgcgtg cggacgcttc cgacagctcg gtgtatgaat tcatgcgcat tatccaggat
     4981 tacgcgacga acggcatcga cacggcggag ctcagctata cgaaacgcgc cgtctgcaac
     5041 ggtgaagcgt tgcggtatga agatccgcgg cagaagctca ttttcctcaa gcgcatcatc
     5101 gattacaatc ttcccgtcga ctattccga
//
LOCUS       DASTSF010000066        12992 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1074003,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000066 DASTSF010000000
VERSION     DASTSF010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 12992)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12992)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12992
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1074003"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..487)
                     /locus_tag="VFU15_01970"
     CDS             complement(<1..487)
                     /locus_tag="VFU15_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003005370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEU4716564.1"
                     /translation="MTTSATAGLPVEKFAYDNKIVKWFAYATILWGIVGMTVGLWVAL
                     DLVFPQLNLGLSFTTFGRTRPLHTNAVIFAFVGNGIFMGVYYSLQRLCKARMYSDLLS
                     KLHFWGWQLIIVSAALTLPLGFTTGKEYAELEWPIDIMIALVWVIFGINMFGTIIKRR
                     ER"
     gene            complement(484..669)
                     /gene="ccoS"
                     /locus_tag="VFU15_01975"
     CDS             complement(484..669)
                     /gene="ccoS"
                     /locus_tag="VFU15_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008257775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HEU4716565.1"
                     /translation="MSAIFIMIGASLLVASGFLFAFIRAVKSGQYEDDYTPGVRMLFD
                     DEVKTKPSNNEKPSTGL"
     gene            complement(731..3160)
                     /locus_tag="VFU15_01980"
     CDS             complement(731..3160)
                     /locus_tag="VFU15_01980"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase
                     metal-binding domain-containing protein"
                     /protein_id="HEU4716566.1"
                     /translation="MKITPASLTVDKRVTCYHCGEVCPPVPVNEDGHDFCCEGCHLVY
                     DLLKQNDLCSYYDISKAPGRAAEDAEKNAVYASLDEPEVRSRMLRFEDEKTARVVFHV
                     PSMHCSSCIWLLEHLYRMETGILSSTVNFPAREVSVDFDPRKIKLSRVAALFAATGYA
                     PSISLGDLEISAKKKRFNPRVLKIGVAGFCFGNIMLLSFPEYLSLGDLHEVPALRTFF
                     AWLSLALSIPVIAYSASEFFIFAWKAIRFRSLNIDAPVALAISVTFLRSVYEITTGSG
                     PGYLDSMSGIVFFMLIGRYFQDRTHENLSFERDYRSYFPIAVTALKNGKEISLPVTRL
                     KAGDRIIIRNGELVPADCILLDGNAEIDYSFVTGESEPVTKENGEKIFAGARQTSEAI
                     TLEVLKETSQSYLTQLWNNDSAGRRNVEEHKSYIDRVNRWFTLGVLLLASSASVVWLF
                     IQPSNALNVFVSVLIVACPCTLLLASTFTNASVLHWFGKYGLYLKNSAVIEKLATASA
                     IVFDKTGTITEGNEGSTAYEGKPLSAEETAALALLASQSGHPLSRRIAAVLKKGNGRI
                     TGYSDHKGKGISGNIGGRFYMIGSADFIGEKKEAKTAEAWIAIDGKVKGKFVFRNRYR
                     AGLAEIISALTEKFSTELLSGDNEAERENLQPVFGNEMRFNQSPAEKLERIRVLQDEG
                     KKVIMVGDGLNDAGALLRADAGIAVSDSMNNYFPACDAILDGKSFARLPLLLRYSRIA
                     RRIIIVTFLFSVIYNIAGIYFSVRGELSPVIAAVLMPASSLSVILITTLSSRISAKRL
                     FGAADGNHTAV"
     gene            complement(3129..3581)
                     /locus_tag="VFU15_01985"
     CDS             complement(3129..3581)
                     /locus_tag="VFU15_01985"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEU4716567.1"
                     /translation="MNIFLDYKTDIALLLARLVLGVLFFMQAYDKIFGLGLKRTEEGA
                     EEALERTKLPHGLIRFFTALTAFLELGGGILLIAGLYIYPALTLLSVDLLFVVFAMSL
                     KEPMWDMRFVWPRLVLLLLLLMAPQQWDRISFDHLLQIYENHPGIING"
     gene            complement(3613..4014)
                     /locus_tag="VFU15_01990"
     CDS             complement(3613..4014)
                     /locus_tag="VFU15_01990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716568.1"
                     /translation="MKMAIHDALTTGEIRKDFSARFPFLRIEFFRSGTGKKYSPSERI
                     SDGETVIGTFRKKHVDGALVIDAGETVSELEKNFREQFGLNVQVMRRAGNTWLMTTST
                     DTMPLENLDELGAELAGPPETGEPVDIHEQS"
     gene            complement(4159..4593)
                     /locus_tag="VFU15_01995"
     CDS             complement(4159..4593)
                     /locus_tag="VFU15_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015754249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEU4716569.1"
                     /translation="MATMLKPKENFSSLGELFDVDRFFGNPWFREVISKAFPAVNIRE
                     TAKDYSIEFAVPGFKKEDFKIRVEKDVLIVSADEKEEKKEDKETYTRREFSFHSFSRS
                     FILPESAERENIEAKYENGILKIVVSKKQSAPVPAKKEIQVS"
     gene            complement(4683..5519)
                     /locus_tag="VFU15_02000"
     CDS             complement(4683..5519)
                     /locus_tag="VFU15_02000"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEU4716570.1"
                     /translation="MKPLVIAIDYSPAGRNAAEYGVQLAKQLQMPVFLFHSYALPVLS
                     GESVAVPVSPEEIEQSQAEAIRDEAGRLEKKYGVKIATGQSMGFAAEEIGEFAKAHSA
                     GLIVAGMQHPNTAGKILGSVTTALIGEAEFPVLVVPENATFAAPKNILFATDLKTNSE
                     WKELDFLKELNAKQGVQIHIVNAVSKDHVPDQAESIEGIHLENKVKEISHDWYFPEND
                     HLPEAIAETAVKVKAEWIAVVHHRLPVMKQVFHRSSAKKLAFNLTLPMLALPEKHAGI
                     LG"
     gene            complement(5516..6043)
                     /locus_tag="VFU15_02005"
     CDS             complement(5516..6043)
                     /locus_tag="VFU15_02005"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEU4716571.1"
                     /translation="MKRSIATGMQEYSKLNPVELKEVIVSKFHETIRDTIGMAETQLA
                     AAGKIEKTEEVEVVMNFLEKLKHSFEQHAMREERLLFPLLSLESREEAIREIEEIGTF
                     ISELKKEHENMERELDLIKMATNNYQCDPMASPSHKLAYAHLNDVGQDFRRMFFIEEE
                     FLFPRILRFNLKKQK"
     gene            6194..6532
                     /locus_tag="VFU15_02010"
     CDS             6194..6532
                     /locus_tag="VFU15_02010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716572.1"
                     /translation="MFTVLTDDIEVFRILEIAGRATFDMRKNRVDDFILDDLYFLLIF
                     HPVGIQKGVHLFSVRMAEIGRVPGLQLLDALLAFPENDGLGPVKSEALALIAKRISER
                     NLGREFFDAH"
     gene            6663..>12992
                     /locus_tag="VFU15_02015"
     CDS             6663..>12992
                     /locus_tag="VFU15_02015"
                     /inference="COORDINATES: protein motif:HMM:NF036933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716573.1"
                     /translation="MIKKATHPRLPLFLVFFITALITAPGCLFAQSGNEFWLAPPDIT
                     DLHNPPGGEPLYLELSSTGSASTVTIDQPANGSFTPIVVTVQANKSLRVNLTAFKTSL
                     ETRPTNTICNTGLHVTSTTPITCYYECANTNNTDIWALKGPNALGTQFYIPLHKHAPF
                     HNEASFSSPNQAFASFDICATQNGTVVQIYSPTQVDGHAALQQFSITLNRGQTYSCGW
                     TGANWDQPSTHPSGAVVISSQPVTVSLKDDSDHNPSGGCYDILGDQIVPTNVVGEDYI
                     AVKGSLNNTGDESVVLMATQNNTQVFLDGNATPVATLFAGEYFRIDIDSLSASTNNAI
                     YIHCTQPTYAMHITGFGCEMGMAELPPLNCAGSQALNFVRDDAQTFYITLLCRTAAVN
                     SFTITGPGTATINPASFVTVPGTGGTWKAARIQYNTTQVPVDSTFHISNSVDVFALGV
                     INGGAATGCKYGYFSEFVAPITVNAGIDQTICANTSATMAGVIGGGTTTGIWTTSGTG
                     TFAPSNTALNAVYTPSAGDASLGSVTLTLTSTGACNPVSDQMVLTITPAPTVNAGPDQ
                     SVCRNNSNVSLNGSVTVATGGVWTGGAGTFVPNNSTLNAVYIPTAAELAAGTLVLTLT
                     TTGNGICNPTSDNMVVTFTNAPVVGAGSDQTKCANNPNTTLAGSVSGATGGVWSGGAG
                     SFTPTPTTLNAVYTPTGTEISSGSVTLTLTSTGNGTCNAVTDQMTIFFTAAPTANAGP
                     DQTKCANNATTTLAGSVTIASGGQWSGGLGTFAPSNNALNATYTPTAAEISAGSVTLT
                     LTTTGNGSCSSVTDQMTIFFTPAPTVNAGVDQTVCANNSTVTLNGSFTVASGAVWTGG
                     AGTFTPNNTTMNATYTPTAAERAAGTVTLTLTTTGNGTCSSVSDQMIITITPSPTVNA
                     GSDQTKCANNPATVLAGSVTVASGGQWSGGAGTFSPNNTTLNATYTPTAAEISGGSVT
                     LTLTSTGNGSCNSVTDQMTIFFTPAPTVNAGPDQTKCGNNATTILAGSVTVASGGQWS
                     GGLGIFTPNNNTLNASYTPTAAEIASGSVTLTLTTTGNGSCSSVTDQMTIFFTPAPTV
                     NAGPDQVVCANNPVVTLNGSFTIASGVAWSGGAGTFSPGNTTPNATYTPTAGEIAAGS
                     VTLTLTTTGNGTCNSVSDQVLITISPAPVVNAGADINSCANNSTVSLNGQVFNAGGGQ
                     WSGGSGTFFPNSTTLNATYTPSAAEISAGSVTLTLTSTGNGTCSAVSDQVVISYAPSP
                     VANAGSDQTVCGNNATVSLGGFVNFCAGGQWSGGLGLFSPSATSLNATYTPTAGEIAS
                     GFVNIILTTTGNGSCNPARDTMRINFTPAPTADAGAPVTSCANNSAVTLNGTITVASG
                     GLWSGGAGSFSPSNTALGATYTPSASEISGGGVTLFLTTTGNGTCLPVSDSVHITITP
                     SPVVNAGVDQTVCVSNMNANLSGSISGPTNTGTWTTSGSGTFTPNNTTLNAAYHCSAA
                     DSLAGSVTLTLTSTNNGSCNAVSDQMVITILPAGSSNAGSDIVVCANNGTISLSGTIG
                     GGATGGTWSSSGTGVFTPNASSLNANYIPSSFDIANGGATIYLTANSCNPAVDSLLLT
                     ITPAPVVNAGSDMIVCAATPDASLNGTISGGSSTGVWTTSGSGTFTPSSSALNATYSP
                     SAADIAAQNVYLVLTSTNNGSCSPVSDTMILHIYPTGTANAGPDQIVCSNNPNISLNG
                     ALGGGATQGQWSTTGTGSFIPNDTTLNATYVPSSADISSGSLSLVLTATNSCNNAVDF
                     LNVIFSPAPVVDAGSPQSTCGANPSVILNGSVSNATGGVWSSGGGTFAPNNTTLNATY
                     SPDAAEQAAGYVILTLTSTGNGNCNAVSDTVRLTFSPGIVANAGVDQSVCISASYTQL
                     NGIVSNGSTTGVWSTLGSGTFTPNDSALNAVYNFSTADTAAGSVTIVLSSTNNGTCPV
                     AHDTMHITFGPSAFAYAGMDQSVCSSDATIQLNGFVAGGATQGMWTTSGGGTFSPNDS
                     TLNAVYTLTPSDIAAGQLTFILETTDHGGCFPGIDTVMYFIAPTSVVSAGGDVTICSD
                     NLSVPLGGNVSGASSTGMWTTSGNGAFIPNNTALNG"
BASE COUNT         3067 a         3677 c         3276 g         2972 t
ORIGIN      
        1 gacgttcgcg gcgcttgatg atcgtcccga acatgtttat tccgaagatc acccacacca
       61 gcgcgatcat gatgtcgatc ggccactcga gctctgcata ttctttcccg gttgtaaaac
      121 cgagcggaag cgtaagcgca gcggaaacga tgatgagctg ccatccccag aagtgcagct
      181 tgctgaggag gtcgctgtac attctcgctt tgcacaggcg ctgcagggaa taataaacgc
      241 ccataaagat cccgttgccc acaaaagcga agatcaccgc attcgtatgc agcggtcgtg
      301 tccgcccgaa cgtggtgaag ctcagcccga gattaagctg cgggaataca aggtcgagtg
      361 cgacccacag gcccaccgtc attcccacaa ttccccagag gatcgtggca tacgcgaacc
      421 attttacgat cttgttgtcg tacgcgaatt tttcaaccgg aagtccggcg gtagcagagg
      481 ttgtcataaa ccggtggagg gtttttcgtt attggaaggt tttgttttta cttcatcgtc
      541 gaagagcatg cgtacaccgg gagtgtaatc atcttcgtac tggccgcttt tcacggcacg
      601 gataaatgcg aaaagaaatc ccgaagcgac gaggagactt gctccgatca ttatgaaaat
      661 ggcactcatt tcagcggatt taaatcggtt caaaattatc cgcgcgatga agtgtgactt
      721 atgactgtat tcagacggcc gtatgatttc cgtcagcagc gccgaacagg cgtttggcgg
      781 aaatgcggga agaaagcgtg gtgatgagaa tgacgctgag tgagctcgcc ggcatgagca
      841 ccgccgcaat aaccggggaa agctcgccgc gcacggaaaa ataaattccg gcgatattgt
      901 agatgacaga aaacaaaaag gtcacgataa tgatcctgcg cgcaatgcgt gaatagcgga
      961 gaaggagggg aagccgtgcg aatgatttcc cgtcgaggat cgcatcgcac gcaggaaaat
     1021 aattgttcat gctgtccgaa acagcgatgc cggcgtcggc acggagcagc gcccccgcat
     1081 cgttcagtcc gtcgccgacc atgatcactt tttttccttc gtcctgtaaa acgcggatgc
     1141 gttcgagctt ttccgccggc gactggttga atcgcatttc gttcccgaaa accggctgca
     1201 ggttttcccg ttccgcttcg ttgtcgccgg aaagaagctc tgtgctgaat ttttccgtaa
     1261 gcgcgcttat tatttccgcg agaccagcgc ggtacctgtt cctgaaaaca aatttccctt
     1321 ttacttttcc gtcgatcgcg atccaggctt cagctgtttt tgcttctttc ttttccccga
     1381 taaaatccgc agagccgatc atgtaaaaac gtccgccgat atttccgctg atgccttttc
     1441 ccttatgatc ggaataaccg gtgatccggc cattgccctt tttcagcaca gcggcaattc
     1501 gcctgctcag cgggtggccc gactgtgaag cgagcagcgc aagtgcagca gtttcttccg
     1561 cggaaagcgg ttttccttcg taagccgtgc ttccttcatt cccctccgtg atcgtgcctg
     1621 ttttatcgaa cacgatcgcg ctggcagtgg ccagcttttc aatgacggcg gaattcttca
     1681 ggtataatcc gtatttcccg aaccaatgca atacgcttgc attcgtaaat gtggaggcga
     1741 gcagcaatgt gcaggggcac gcaacaataa gcaccgaaac aaaaacattc agcgcattcg
     1801 agggctggat gaaaagccat acgaccgaag ctgaagacgc gaggagcagc acgcccagcg
     1861 taaaccagcg gttgaccctg tcgatatagc ttttatgctc ttccacgttc ctgcgtcccg
     1921 cggaatcgtt gttccacaat tgcgtcaggt aactctgcga tgtttctttc agcacttcca
     1981 gcgtgatcgc ttccgacgtc tgccgtgcgc cggcaaatat tttttctcca ttttccttcg
     2041 tcacaggttc tgattcgccg gtgacaaagc tgtagtcgat ctcggcattc ccgtcaagaa
     2101 gaatgcaatc ggccggcacc agctccccgt tgcgtatgat gatccggtcg cccgctttga
     2161 gccgggtcac cggcaggctg atctcttttc cgttcttcaa cgcagtaaca gcgatcggaa
     2221 aatacgagcg gtaatcgcgt tcgaaggaaa gattttcatg cgtgcggtcc tggaaatacc
     2281 tcccgatgag catgaaaaaa acgatcccgc tcatcgaatc gagatatccc gggccgctgc
     2341 ccgtcgtgat ctcatacacg ctgcgcagga acgtgacgct gatcgcgagc gcaacgggcg
     2401 catcgatgtt caggctgcgg aaacggatcg ctttccatgc gaaaataaaa aattcggaag
     2461 cgctgtatgc aattaccggg atcgacagcg caagactgag ccacgcgaaa aaagttctca
     2521 gcgcaggaac ttcatgcaga tcgccgaggg aaagatattc cggaaagctc agcagcatga
     2581 tattcccgaa gcagaatccc gcaacgccga tcttcagcac acgcggattg aatctttttt
     2641 tcttcgcgct tatttccagg tcgccgaggc tgatcgaagg agcgtagcct gtcgctgcaa
     2701 ataaagcggc aacacggctg agctttatct tccgcggatc gaaatccacc gaaacttcgc
     2761 gggcaggaaa attgaccgtg gaagacagga tgcccgtctc catccggtaa agatgttcga
     2821 gcagccagat gcacgagctg caatgcatgg atggaacatg aaaaaccacg cgggccgtct
     2881 tttcatcttc gaaacgaagc atgcggcttc ttacttccgg ttcatcgagt gaagcgtaaa
     2941 cggcattctt ttccgcatct tcagcggcgc gaccgggcgc ttttgaaata tcataatagc
     3001 tgcagagatc attctgcttc agcagatcgt aaacgaggtg acaaccttca cagcagaaat
     3061 catgtccgtc ctcgttgacc ggcaccggcg ggcaaacctc tccgcaatga tagcacgtta
     3121 cgcgtttgtc aaccgttaat gatgccgggg tgattttcat agatctgcag caggtgatcg
     3181 aaagagatgc ggtcccattg ctggggcgcc atcagcaaaa gaagaagaag gacgagacgc
     3241 ggccacacaa aacgcatatc ccacatcggc tctttcaggc tcatcgcgaa aacgacgaac
     3301 aggagatcca cgcttaacaa ggtcagcgcc ggataaatgt aaagccccgc gatcagaagg
     3361 atcccgcctc ccagctccag gaaggccgtg agcgcggtaa aaaaacggat gagcccgtgc
     3421 ggcagcttcg ttcgttcaag cgcctcttct gcgccttctt cggtgcgttt caggccaagt
     3481 ccgaaaattt tgtcatacgc ctgcatgaaa aaaagaacgc ccagcacaag ccgtgcgagc
     3541 agcagcgcga tgtcggtttt ataatcaagg aaaatattca tggttcagcg ggttttgaga
     3601 gaatggtttt tgtcaactct gctcgtggat gtcgaccggc tcccccgttt ccggcggacc
     3661 ggcgagttcg gcgcccagct catccaggtt ttccagcggc atcgtatcgg tgctggttgt
     3721 catcagccag gtattgccgg ccctgcgcat gacctgtacg ttcagtccga attgctcccg
     3781 gaagtttttt tccagctccg aaacggtttc tcctgcatcg attacgagcg cgccgtcgac
     3841 gtgttttttc cggaatgtcc cgataaccgt ttcaccgtcg gaaatgcgtt ccgaaggaga
     3901 atactttttc cctgttccgc tccggaaaaa ttcaatccgc agaaaaggga aacgggcgga
     3961 aaaatccttc cggatctccc ccgtcgtcaa cgcatcgtga atggccatct tcatttttct
     4021 gtttctaaaa atccgccggg cggggtgcgt accaggcagc gttcttcggt tcaatatgcc
     4081 cacccgctcc gtggcagagg gatccttctg cacgtacacc ccggcatgaa atgaacctgt
     4141 ttcaataaga tcctgttttc agcttacctg tatttctttt tttgcgggaa caggtgcgct
     4201 ttgttttttc gagacaacga ttttcaggat cccgttttca tatttcgctt cgatattttc
     4261 acgttccgcg ctttccggta aaatgaacga acgggaaaaa gaatggaaag aaaattccct
     4321 tcgggtatac gtctctttgt cttccttctt ttcctctttt tcgtctgcgg aaacaatcag
     4381 gacatctttt tcaaccctga tcttgaaatc ttcttttttg aatcccggca ccgcgaactc
     4441 gatcgaatag tctttcgccg tttcacggat attgacagcc ggaaaagctt tggaaataac
     4501 ttccctgaac cagggatttc cgaaaaagcg gtcgacatca aaaagttctc cgagagagga
     4561 aaagttttct ttgggtttga gcatagttgc catgatgaag attttttgag tttgttgatg
     4621 caaaaatgcc gcgcgcgcgg atgtttttaa atgagtggcg tcaaatacct gcctgattct
     4681 tatcagccca ggattcccgc gtgtttttca ggcagggcaa gcataggcag tgtcaggtta
     4741 aatgccagct ttttcgcgga actgcggtga aacacttgct tcattacggg caggcgatga
     4801 tgaacaaccg cgatccactc tgcttttact tttacagcgg tttccgcgat cgcttccggc
     4861 agatgatcgt tttccgggaa ataccagtcg tgcgaaattt ccttcacttt gttttccagg
     4921 tgaatccctt cgatgctttc cgcctggtcg ggtacatgat ctttggaaac tgcattcacg
     4981 atgtgtatct gcacaccttg cttcgcattc agttctttca ggaaatccag ttccttccat
     5041 tctgaatttg ttttcaggtc cgtggcaaaa agaatgttct tcggtgccgc gaacgttgca
     5101 ttttcaggca cgacaaggac cgggaactcg gcctcgccga taagcgcagt cgttacactc
     5161 ccgaggatct ttcctgccgt gttcggatgc tgcatgcccg caacgataag tccggccgaa
     5221 tgcgctttcg cgaattcccc gatctcttca gctgcgaaac ccatgctctg tccggtagcg
     5281 atctttacgc cgtatttttt ttcgaggcga ccggcttcgt cgcggatcgc ctctgcctgg
     5341 ctctgttcga tctcttccgg agaaacaggc acagcgaccg attctcccga taacacggga
     5401 agcgcgtagg aatgaaaaag aaaaacgggc atctgcagct gctttgcaag ctgcaccccg
     5461 tattcggcag cgtttctgcc tgccggtgaa taatcgattg caatgacgag cggtttcatt
     5521 tttgtttttt taggttgaat cttaatatgc ggggaaaaag aaattcttct tcgataaaaa
     5581 acatccggcg gaaatcctgg cccacatcgt tcaggtgtgc gtaggccagc ttgtgcgacg
     5641 gagatgccat cgggtcacac tggtaattat tcgttgccat ttttatcagg tcgagctcgc
     5701 gttccatatt ctcatgctct ttcttcagct cgctgataaa tgttccgatc tcttcgatct
     5761 cccggatcgc ttcttcgcgc gactcaagag aaagcagcgg aaacagcagg cgctcttcac
     5821 gcatcgcatg ttgttcaaaa ctgtgtttca gcttttcaag gaaattcatc acgacctcca
     5881 cctcctccgt tttttctatt ttgccggcag cagccagctg cgtttcagcc ataccgatcg
     5941 tatcccggat cgtttcatga aattttgaaa caataacctc tttcagctct acaggattta
     6001 actttgaata ttcctgcatc ccggtagcta tacttctttt catgttatca aaagtagacc
     6061 ggaaggcggg caggactaat gatcgcgctc aacggagcag gtgagtaatg tcagattgtt
     6121 tttccggaaa caggcattgc gcccgggctg caggcggtct tttgaatgcg tatgaagcat
     6181 cataaaaaaa gaaatgttca ctgtactgac tgacgacatc gaggttttcc gcatcctgga
     6241 aatagccggc cgggcaactt tcgacatgcg caaaaaccgc gtggacgatt tcattctcga
     6301 cgacctgtat ttcctgctca tctttcatcc ggtcggaata caaaagggag ttcacctgtt
     6361 cagcgtgcgt atggctgaga ttggtcgcgt tccggggttg cagctgctcg atgcattgct
     6421 tgcttttccg gaaaacgatg gactgggtcc ggtcaaaagc gaggcgcttg cgctgatcgc
     6481 gaagaggatc tctgaaagaa atctcggcag agagtttttc gatgcgcatt gatcacaacc
     6541 ggtttagtct ttgcagaata gccttacggc gatgatttag acctgttttt ccaaattcta
     6601 acaaccattt tttatctcct gccgtataac tacgacactg tacaccatta ccaggaggtt
     6661 tgatgattaa aaaagctact cacccgcggc ttccgctgtt tcttgttttt ttcataaccg
     6721 cgcttattac agccccaggc tgcctgtttg cgcaaagcgg aaatgaattc tggctcgcac
     6781 cgcccgatat taccgatctt cataatcctc ccggcggcga gcctctttat cttgaacttt
     6841 cttctaccgg gtcagcctcg accgtcacca ttgatcagcc tgcgaacgga tcttttaccc
     6901 cgatcgttgt aactgtgcag gcgaataaat ccctgcgggt gaacctgacg gcattcaaga
     6961 cctcgctcga aacgcgcccc acgaatacga tctgcaatac aggtcttcac gtcacatcca
     7021 caacgccgat cacgtgctat tatgagtgtg ccaatacgaa caacaccgac atctgggcgc
     7081 tgaagggacc caacgcgctc ggtacgcagt tttatattcc gctgcacaag catgctccgt
     7141 tccacaacga agcatcattt tcttctccca accaggcttt tgcttctttc gatatctgcg
     7201 ccacgcagaa cggaacggtc gtgcagatct attctcccac acaggtggac ggacacgcag
     7261 cgctgcagca gttctccatc acgctcaacc ggggccagac gtattcctgc ggctggacag
     7321 gagccaactg ggaccagcca tcgacgcatc cctccggcgc cgttgttatt tccagccagc
     7381 cggtcaccgt ttcactgaaa gatgattcgg accataatcc ttccggcggc tgttatgata
     7441 ttctcggcga ccagatcgtt cctacaaatg ttgtgggaga agattacatc gccgtcaaag
     7501 gttcgctgaa caatacggga gatgaatcgg tagtgctcat ggcgacgcag aacaacacgc
     7561 aggtgtttct cgacggaaac gccacgccgg ttgcgacgct tttcgccggg gaatatttcc
     7621 gcatcgacat cgattcatta tccgccagca ccaataacgc tatttacatt cattgcacgc
     7681 agccgacata tgccatgcac atcaccggtt tcggctgtga aatgggaatg gcagaattgc
     7741 ctccgctgaa ctgtgcggga tcacaggcgc tgaattttgt ccgtgatgat gcacaaacat
     7801 tttacatcac gttgctgtgc cgtaccgctg ccgtaaattc attcacgatc acaggtcccg
     7861 gaacggcaac catcaacccg gcttcttttg tgactgttcc cggaaccggc ggaacatgga
     7921 aagctgcacg gatacaatac aacaccacgc aggtaccggt cgattcgaca ttccatattt
     7981 caaactccgt ggatgttttc gctttgggcg tcatcaatgg cggagctgcg accggatgta
     8041 aatacggtta cttttccgaa ttcgtcgcac cgatcacagt caatgccggg atcgatcaga
     8101 ctatctgcgc caacacttcg gctacaatgg ccggtgttat tggcggcgga acaacaaccg
     8161 gcatctggac aacttccgga accggaacat ttgcgcccag caacaccgcg ctcaatgccg
     8221 tttatacgcc aagcgcagga gatgcttccc tgggaagcgt tacactcacg ctgacatcaa
     8281 ccggcgcctg caacccagtc tccgaccaga tggtgcttac catcacaccg gctccaaccg
     8341 tgaatgccgg gcccgatcaa tccgtgtgca ggaacaattc caacgtttcg ctgaacggaa
     8401 gtgtgacggt ggcaacaggt ggcgtgtgga ccggtggcgc gggaacattc gttccgaaca
     8461 acagcacgct taatgccgtt tatattccta ctgcggcaga acttgccgca ggaacgcttg
     8521 tgcttacact cacaacgaca ggtaacggca tctgcaatcc caccagcgat aacatggtcg
     8581 ttacgttcac gaacgcgcct gtagtcggcg caggatccga ccagacaaaa tgcgcgaaca
     8641 accccaacac tacgcttgcc ggttctgttt ccggcgcaac gggtggtgtg tggtcaggtg
     8701 gcgcaggatc attcacacca acgccgacaa cactgaacgc cgtttatacg ccgacgggca
     8761 cggaaatctc ttcgggcagc gtcacactta cgcttacatc gaccggcaac ggaacgtgca
     8821 acgcagtgac ggaccagatg acgatcttct tcaccgccgc accgaccgcc aatgccggac
     8881 ctgaccagac caagtgcgcc aacaatgcca cgacgacact tgccggaagc gtgaccatcg
     8941 caagcggcgg acaatggtcg ggcggactcg gaacatttgc accgagcaac aacgcgctga
     9001 atgcaactta tacacctaca gccgcagaaa tttctgcagg cagcgtgacg ctgacactta
     9061 ccacaaccgg caacggatcc tgctcttccg tcaccgacca gatgacgatc ttcttcacgc
     9121 cggcaccgac cgtaaatgcg ggcgtagatc aaaccgtttg tgcaaacaac tccactgtta
     9181 cgctcaacgg atctttcact gtggcaagcg gtgctgtgtg gaccggaggt gcgggaacat
     9241 tcacgccgaa caacacaacg atgaatgcga cgtacacacc taccgcagcc gaacgcgcag
     9301 cggggaccgt gacattgaca ttgacaacga cagggaacgg aacgtgcagc tccgtcagcg
     9361 accagatgat catcacgatc actccttctc caacggttaa cgcaggttcc gaccagacca
     9421 aatgcgccaa caacccggcc accgttcttg ccggttcagt aactgtcgca agcggcgggc
     9481 aatggtcggg tggagcggga acgttcagcc cgaacaacac cacgctcaat gcaacataca
     9541 cgccgacggc cgcggaaatt tccgggggaa gtgtaacgct cacgcttaca tcgaccggga
     9601 acggatcctg caattccgtc accgaccaga tgacgatctt cttcacgccg gcaccgaccg
     9661 tgaatgcagg acccgatcag accaaatgcg gaaacaacgc aaccaccatt cttgccggtt
     9721 cagtaactgt cgcaagcgga ggacaatggt cgggcggtct cggcattttc acacccaaca
     9781 acaacacgct caacgccagc tatacaccca cagctgctga gatcgcatca ggaagtgtaa
     9841 cacttacgct tacgacaacc ggcaacggat cctgctcttc cgtcaccgac cagatgacga
     9901 tcttcttcac accggcgcca acggtgaatg cgggacctga ccaggttgtc tgcgccaaca
     9961 atccggttgt aacgctcaac ggatcattca cgatcgcaag cggagttgcg tggtcgggcg
    10021 gagcgggaac attctctccc ggcaacacaa ctcctaacgc cacatacaca ccaacggccg
    10081 gcgaaattgc tgccggttcg gttacgctga cgctgacaac aaccgggaac ggaacctgta
    10141 attcggtcag cgaccaggtg ctcattacta tttctcctgc accggtggta aatgccgggg
    10201 cggacatcaa ctcgtgcgca aacaactcaa ctgtatcgct caacggccag gtgttcaatg
    10261 ccggcggcgg acaatggtcg ggaggaagcg gaacgttctt ccccaacagc acaacactta
    10321 atgcaaccta tacgccgagc gccgcagaaa tctctgcggg aagcgtgaca ttgacactga
    10381 cttctaccgg caatgggacc tgcagcgccg tcagcgacca ggttgtgatc tcctatgcgc
    10441 catcacctgt tgcaaatgcg ggaagcgatc agacagtatg cggaaacaac gcaacagtga
    10501 gcctgggcgg tttcgtgaat ttctgcgcgg gcggacaatg gtcgggagga ctcggattat
    10561 tctctccttc ggccacttcg cttaatgcga catacacacc gacagcaggc gagatcgctt
    10621 cgggttttgt gaacattatt ctcaccacca ccggtaatgg ttcctgcaac cccgcacgcg
    10681 atacgatgcg catcaatttc acaccggcac ccacagccga cgccggggcg ccggtcacct
    10741 cgtgcgcaaa taattctgcg gtcacgctga acggaacaat aaccgtcgcc agcggtggat
    10801 tatggtcagg cggagcagga tcgttctcgc cttcgaatac cgcgctcggc gcaacgtata
    10861 caccttccgc aagtgaaatt tccggtggag gcgtgacatt gttcctgacg acaacaggca
    10921 acggaacctg cctgccggta agcgacagcg tgcatattac cattacgccg tctcctgttg
    10981 tcaacgcagg agtcgaccag accgtttgcg tcagcaacat gaacgcaaac ctcagcggaa
    11041 gcatttcggg tccgacaaat acaggaacgt ggacaacatc cggaagcgga acattcacgc
    11101 ccaacaatac aacactgaat gccgcttatc attgcagcgc cgccgattcg ctggccggaa
    11161 gcgtgacgct tacactgact tccaccaaca acggttcgtg caacgctgtt tccgaccaga
    11221 tggtgatcac catacttccg gcgggaagct ccaatgcagg aagcgatatt gtggtttgcg
    11281 cgaacaacgg aacgatctcg ctcagcggaa cgatcggcgg aggagcaacg ggcggaacct
    11341 ggtcttcttc cggcacgggt gtgttcacgc cgaatgcttc ttccctcaat gcgaattaca
    11401 ttcccagttc attcgacatc gccaacgggg gcgcaaccat ttatctcacc gcgaacagct
    11461 gcaatcctgc tgtcgacagc cttctcctta cgatcacccc ggcccctgtg gtcaacgccg
    11521 gcagcgatat gatcgtttgt gctgcaacac cggacgcttc gcttaacggc accatcagcg
    11581 gcggttcttc cacgggtgta tggacgactt ccggaagcgg aacattcacg ccttccagct
    11641 ctgcgcttaa tgcaacctac agtccgagcg cagccgacat tgccgcacaa aacgtttacc
    11701 tcgttctcac ttcaacgaac aacggaagct gctcgccggt ttccgacacg atgatcctgc
    11761 acatttatcc gacgggaacc gcgaatgccg gacctgacca gatcgtctgc tcgaacaatc
    11821 cgaatatttc gctgaacgga gcattgggcg gaggcgcaac gcaggggcaa tggagcacga
    11881 ccggaacagg atcattcatt ccgaatgaca cgacgttgaa cgcgacgtac gttccgagca
    11941 gcgcggatat ttcttccggc tcgctaagcc ttgtgctcac cgcaaccaac agctgcaaca
    12001 atgccgttga cttcctcaat gtgatcttct cgcccgctcc ggtcgttgat gcgggaagcc
    12061 cgcagtcgac ctgcggcgcc aacccgagcg tgatcctcaa cggttccgtt tccaatgcaa
    12121 cgggaggcgt ctggagcagt ggcggtggaa cattcgcgcc gaacaacacg acgctcaatg
    12181 caacctattc gccggatgca gccgaacagg cagcgggtta tgtgatcctt acgctcacct
    12241 ccaccggcaa cgggaactgc aatgctgttt ctgatacggt gcggcttaca ttctccccgg
    12301 gaatcgttgc caatgcgggc gtggatcaat ccgtgtgcat cagcgcatcg tacacgcagc
    12361 tgaacggcat cgtgtcgaac ggctcgacaa caggtgtgtg gtcaacactc ggctcgggaa
    12421 cattcacacc gaatgacagc gcgctgaacg cggtttacaa tttcagcacg gcagataccg
    12481 cagccggttc ggtaacgatc gtgctttctt ccaccaataa cggaacatgt cccgtcgcgc
    12541 acgatacgat gcacatcacc ttcggaccaa gtgcattcgc gtatgcagga atggaccagt
    12601 cggtctgctc ttccgatgca acaatccagc tcaacggttt cgtggcaggc ggcgcaacgc
    12661 agggaatgtg gacaacctcc ggcggcggaa cattctcccc gaatgattcc acgctgaatg
    12721 ccgtttacac gctgacacca tcagacattg ctgcgggaca actcactttc attctcgaaa
    12781 ccactgatca tggcggctgc ttccccggta tcgataccgt catgtatttc atcgcaccga
    12841 cttccgtcgt gagtgcgggc ggagatgtta ccatttgttc ggacaacctt tccgttccgc
    12901 tgggcggaaa cgtgagcgga gcctcttcca ccggcatgtg gacaacctcc ggcaacgggg
    12961 cattcattcc gaacaacact gcgctcaacg ga
//
LOCUS       DASTSF010000067         4317 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_626744,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000067 DASTSF010000000
VERSION     DASTSF010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4317
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_626744"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="VFU15_02020"
     CDS             <1..207
                     /locus_tag="VFU15_02020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEU4716574.1"
                     /translation="AEVRAMEDLISKQTIEEIEFFIHADPCIPSSCPVCNVKDCPQRK
                     AAFVKTIAWSKDNMLPDAKHTLQS"
     gene            211..1380
                     /locus_tag="VFU15_02025"
     CDS             211..1380
                     /locus_tag="VFU15_02025"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HEU4716575.1"
                     /translation="MKKNGILLFFLLLSICSDAAFIHRDGKLLRDKSNDPVLLRGVNL
                     GGWLLWEGWIWGGKFTAQSAIQKNISAAAGEKNAADFRDYVYRNFIGEADIRAISEMG
                     LNVVRVPFNNRIFDTAQCNAIGWQVLDSLLSWCAKYHVYAVLDMHGAPGGNSPYFTAD
                     PEKPDLWKSEKDKQRTVDLWKRIAARYKDNETVAGYDLLNEPIPASDADLTALYERII
                     SAIRSVDKNHCLFVEGSSFAKKFTMFTSLPDENMCFSFHIYTWFGGKPADKMDDFVSL
                     ENRLNVPIWCGEWGENNYDVITQTRNALEEKQNGFSGWCFWTWKKIPNGYATLCAVEM
                     DETWKPFITWCCKPSKKNTPSAQTANDELKKFEESMLFRNCRIDTNLRKILEVTR"
     gene            complement(1451..2413)
                     /locus_tag="VFU15_02030"
     CDS             complement(1451..2413)
                     /locus_tag="VFU15_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003579096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEU4716576.1"
                     /translation="MEYRRLGKSGLRVSALSFGSWVTFGAQVGDDTAEKCMKLAYESG
                     VNFFDNAEAYASGKSETVMGNILKKMNWDRTSFVVSSKVFWGGKKPNQTGLSRKHVTE
                     ACHAALRRLQVEYLDLFFCHRPDPETPVEETVRVMNDLIAQGKIFYWGTSEWSAEQIT
                     EAFNIAEKNHLAGPAMEQPQYNLFHRERFEKEYRPVFEKYGIGATIWSPLASGLLTGK
                     YNDGDPGGTRIARSDLKWLKERVTGSEAGDKLEKMKKFTALANGLGTTSARLAIAWTL
                     KNPNVSTAILGASKVEQLEENLKSLEALPLLTPEVMGTIEKIFG"
     gene            2664..3680
                     /locus_tag="VFU15_02035"
     CDS             2664..3680
                     /locus_tag="VFU15_02035"
                     /inference="COORDINATES: protein motif:HMM:NF026220.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HEU4716577.1"
                     /translation="MSRFSSVLFFAFLSAVLSAQSITDISDTTVHSSFRGLSVVNDNV
                     VWLSGTKGTVGNSFDGGKTWQWHTVKGFEQLDFRSLYAMDKKKAAIASAGTPAVILTT
                     ADGGATWETRFRSDDTLMFLDGVDFWDNNHGLVFGDPVNGKMFIAETVDGGKTWNEVP
                     EKNRPQLDKGEASFAASNTTMRTVPGGEVWIATGGYRSRLWHSLDYGHHWSDVETPIL
                     QGVLSAGIFSFAIGGKDFLLITGGDYLQDTLTKKNFFYTVDGGAHWLQKSNNPGGYRS
                     CIEKTGKNQFICTGTSGTDIMEGPEWKPLSPAGYNCVRKSRSGSAVYFCGAKGKIGKL
                     VAGG"
     gene            complement(3681..4010)
                     /locus_tag="VFU15_02040"
     CDS             complement(3681..4010)
                     /locus_tag="VFU15_02040"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017220416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEU4716578.1"
                     /translation="MTSETDVIAPTPPPPYYAVIFTSVRTEGDDGYAETARRMEELAR
                     QQPGFLGYESAREGLGITVSYWENEEAITGWKKNSEHLLAQRYGREKWYSHFKVRVCK
                     VERDYGM"
     gene            complement(4017..4093)
                     /locus_tag="VFU15_02045"
     tRNA            complement(4017..4093)
                     /locus_tag="VFU15_02045"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4057..4059),aa:Arg,seq:tct)
     gene            4134..>4317
                     /locus_tag="VFU15_02050"
     CDS             4134..>4317
                     /locus_tag="VFU15_02050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716579.1"
                     /translation="MILVLSNHRWRSRLSGLRFSTESRGGPRMPEAGLRPFYIGRSDA
                     PLLFFYVGVARLELTTP"
BASE COUNT          957 a         1211 c         1137 g         1012 t
ORIGIN      
        1 gcggaagtcc gcgcgatgga agaccttatt tcaaagcaga cgatcgaaga gatcgaattc
       61 ttcattcatg cggatccctg cattccttct tcctgcccgg tatgtaacgt aaaagattgc
      121 ccgcaacgga aagccgcttt cgtgaaaacg atcgcctgga gcaaggacaa catgcttccg
      181 gatgcgaagc atacgcttca aagctgaact atgaaaaaaa acggcattct cctgttcttc
      241 ctgctgctga gcatttgttc ggatgcagca tttattcacc gcgatggaaa attgctgcgc
      301 gataaaagca atgacccggt cctgctccgc ggtgtcaacc tcgggggctg gttgttgtgg
      361 gaaggctgga tctggggcgg aaagttcact gcgcagtccg ccatccaaaa aaatatttcg
      421 gctgctgccg gcgaaaagaa cgctgctgat ttccgcgatt acgtttaccg gaatttcatc
      481 ggtgaagcgg acatacgcgc gatcagcgaa atgggactga acgtcgtccg cgttccgttc
      541 aacaaccgca ttttcgatac ggcgcagtgc aacgccatcg gctggcaggt gctcgattcg
      601 ctgttgtcat ggtgtgcgaa ataccatgtg tacgcagtgc tcgacatgca cggcgcgcct
      661 ggcggaaatt ccccgtattt caccgccgat cctgaaaagc cggacctgtg gaaatcggaa
      721 aaagacaagc aacgcacggt cgatctctgg aaaaggatcg cagcgcgtta caaagacaac
      781 gaaacggtgg ccggctacga tctgctgaat gaacccattc ctgcatccga cgccgacctg
      841 accgcgctat acgaacgcat catttccgcc atccgttcgg tcgataaaaa ccattgcctt
      901 ttcgtggaag gaagcagctt cgcgaaaaaa ttcacgatgt tcacttcgct gccggatgag
      961 aacatgtgct tcagctttca catctacacg tggttcggcg gaaaacccgc tgataaaatg
     1021 gacgatttcg tttcgctgga aaacaggctc aatgttccca tctggtgcgg ggaatgggga
     1081 gaaaataatt acgacgtcat cacgcagacg cgcaacgcgc tcgaagaaaa gcagaacggc
     1141 ttcagcggct ggtgcttctg gacgtggaag aagatcccca acggctacgc gacattatgc
     1201 gccgtggaaa tggatgagac gtggaagccg tttatcacgt ggtgctgcaa accttcgaag
     1261 aagaatacgc cttccgctca gaccgcgaac gacgagctga aaaagtttga agaatcgatg
     1321 ctcttccgca attgccgcat cgacacgaac ctccgcaaga tactggaggt tactcgctga
     1381 aaaaaacggg acgccggttc gtgcgcgttt catttcttca cctcaacgca ggaccttgaa
     1441 cgttgaacgt tcacccgaat attttctcaa tcgttcccat tacttccggc gtcagcagcg
     1501 gcagcgcctc cagcgatttc aggttctctt ccagctgctc cactttcgat gcgccgagta
     1561 tggccgtgct cacattcggg tttttcagcg tccacgcaat ggccagccgc gcggaagtgg
     1621 tgccgagccc gttcgccagc gcagtgaatt tcttcatctt ttcaagcttg tcgcctgctt
     1681 cgctgcccgt tacccgttct ttcagccact tcaggtcgct gcgcgcaatg cgcgtgccgc
     1741 ccggatctcc gtcattgtat ttcccggtta gcagcccgga agccaggggc gaccagatcg
     1801 tggcgcctat gccgtatttt tcgaacacgg gtcggtactc tttttcaaat cgctcgcggt
     1861 gaaataaatt gtactgcggc tgttccatcg ccggaccggc caggtggttc ttctccgcga
     1921 tgttgaaagc ttcggtgatt tgctccgcgc tccattccga tgtgccccag tagaaaattt
     1981 ttccctgcgc gatcaggtcg ttcatcacgc gcacggtttc ctccaccggt gtttcgggat
     2041 cgggacggtg gcagaaaaaa agatcgaggt attccacctg cagccggcgc agcgctgcat
     2101 ggcaggcttc cgtcacgtgc ttgcggctga gccccgtctg gttcggtttt tttccgcccc
     2161 agaaaacctt cgaggaaacc acgaacgaag tgcggtccca gttcattttt ttcaggatgt
     2221 tccccatcac cgtttccgat tttccgctcg cgtacgcttc cgcattatcg aagaaattca
     2281 cgccgctttc atacgccagc ttcatgcatt tctccgccgt gtcgtcgccc acctgcgcgc
     2341 cgaatgtcac ccacgatccg aaggaaagcg cgcttaccct gagcccgctt tttcccagcc
     2401 tgcgatattc catttccata gctaaacagt ttgaaagttg aaaaaagttg aaagtataag
     2461 ttcaatgcaa aagtaccggt taatccggtt ttggcttttc attttgaagc gcatcttcag
     2521 cgcatctcgg ttccggcttc ccggtgcttc gctgcaagtc ctcgcccgcc cgcaggctcc
     2581 gggctttccg ctgcgccccg ggctatgaaa tttactttcg cacgtcaaat catctctgtc
     2641 cgtccatggc tatatttacg ggtatgagca gattttcctc cgtcctgttt ttcgcttttt
     2701 tatccgcggt actttccgcg caatccatca ccgacatttc ggacacgacg gttcacagca
     2761 gcttccgcgg actttccgtc gtcaatgaca atgtcgtctg gctgagcgga acaaaaggaa
     2821 ccgtgggaaa ttctttcgac ggcggaaaaa catggcaatg gcacacggtg aaaggttttg
     2881 agcagctcga tttccgcagc ctgtatgcca tggacaaaaa gaaagccgcg atcgccagcg
     2941 ccggaacgcc ggctgtcatt ctcaccaccg ccgatggcgg cgcaacatgg gaaacgcgtt
     3001 tccgcagcga cgacacgctg atgttcctcg acggcgtcga tttctgggac aataaccacg
     3061 ggctcgtttt cggcgaccct gtgaatggaa aaatgttcat cgcggaaacc gtcgacggcg
     3121 gaaaaacctg gaacgaggtt ccggaaaaaa accgcccgca gctcgataaa ggagaagctt
     3181 cattcgccgc cagcaacacg acgatgcgta ccgttcctgg cggggaagtg tggatcgcca
     3241 cgggagggta tcgctcgcgt ctctggcatt cgctcgatta cgggcatcac tggtccgatg
     3301 tcgaaacgcc gatcctgcaa ggtgttttgt ccgccggaat tttttcattc gccattggcg
     3361 ggaaagattt cctgctcatc accggcggcg attaccttca ggatacgctg acgaagaaaa
     3421 atttttttta cacggtcgac ggcggcgcgc actggctgca gaaaagcaac aaccccggcg
     3481 gttaccgctc ctgcatcgag aaaacaggga agaaccagtt catctgcacg ggcaccagcg
     3541 gaacagacat catggaaggc ccggaatgga aaccgctttc gccggcaggc tataattgcg
     3601 ttcggaaatc gcgttcgggc agcgccgttt atttctgcgg ggccaaagga aagatcggga
     3661 agctcgtcgc gggcggatga tcacattccg taatcacgtt ccactttgca cacacggact
     3721 ttaaaatgcg aataccattt ttcacgcccg taacgttgcg caagaagatg ctcggaattt
     3781 tttttccagc cggtgatcgc ctcttcgttt tcccaatagg aaaccgtgat gccgagacct
     3841 tcccttgcgc tttcatagcc gagaaatccc ggctgctgcc gcgcgagctc ttccatgcgt
     3901 cgcgccgttt ccgcatagcc gtcgtcgcct tccgttcgca cggaagtgaa gatcacggca
     3961 tagtagggcg gaggtggagt cggggcgatc acgtctgttt cgcttgtcat ctgaagtggc
     4021 gatcccgatt ggattcgaac caatgaccta ctgcttagaa ggcagttgct ctatccagct
     4081 gagctacggg atcattgaag ctgaaaagtt ggaaagtttg aaagtgctgc aggatgattt
     4141 tagtgttgag taatcaccgc tggcgttcca gactttccgg cctgcgcttt tcaaccgaaa
     4201 gccggggtgg tccccggatg cctgaagcgg gcctgcgccc cttttacatc gggcgaagcg
     4261 acgccccgct tttatttttt tatgtcgggg tggccagact cgaactgacg accccct
//
LOCUS       DASTSF010000068         2711 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1967418,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000068 DASTSF010000000
VERSION     DASTSF010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2711
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1967418"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..712)
                     /locus_tag="VFU15_02055"
     CDS             complement(<1..712)
                     /locus_tag="VFU15_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009193959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEU4716580.1"
                     /translation="MDKIPHIPFNKPLIVGRELRNMRHAVASGHISSGGKFTGMCMSF
                     FRENYGVPHNILTTSCTAALELAACLLNIREGDEVIAPSFAYVSTVNAFVLRGARIVF
                     ADSQPGFPNIDADAIEPLITGRTKAIVCVHYSGVACDMEKLKAVSEKYRIPLVEDAAH
                     AIDARWKKERLGTIGAFGALSFHETKNITAGEGGMLFVNDSRYAQRAIILSQKGTNRN
                     DFMLGNVDRYEWVDVGSSL"
     gene            complement(725..1888)
                     /locus_tag="VFU15_02060"
     CDS             complement(725..1888)
                     /locus_tag="VFU15_02060"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEU4716581.1"
                     /translation="MRIALLTDGIFPHVIGGMQRHSFYLAKYLAAAGVQVDLYHTIPG
                     GKKEGAFDLFTGEEKKNLRSFVIPFPDFGRMPGHYIRESYEYSCRVWKKLNSETLPDF
                     IYVKGFAGWELLNRKSKGEKLPPVGLNFHGYEMFQPQPGFVSWLQSRFLLRSPVLFNV
                     RHADYLFSYGGKITALIRSLGAGAEKIIEIPTGISREWLFTGTPEVHAPRRFVFVGRN
                     ERRKGLCELHEAIAQLPGTMEFAFSFVGDIPPKKWVKDARLTYHGEIKDSAALKNILR
                     DADVLVCPSFSEGMPNVVMEALASGLAVIATDTGATSEMVNESNGWLISPGDVAQLAG
                     ALEKAITLSPDALAAKKAGAVKHVENNFLWEKVAGLTIEKISRVCLQGSGDVL"
     gene            complement(1923..>2711)
                     /locus_tag="VFU15_02065"
     CDS             complement(1923..>2711)
                     /locus_tag="VFU15_02065"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEU4716582.1"
                     /translation="APFEEEVRGFEKAGSMLAAAGKKRMNELLDDAARVITVSSALAG
                     YLAERYGVKKEKITVTPNCVDPGAVNVSPAKAEQLRRKYFPGKKTVIGFVGSIFPYHG
                     VDILIRAFAELHRQHSETGLFIVGDGYLIPELKELASALGCGDSVVFTGSVPHADIFD
                     HIAAMDITVMADSNWYGSPVKIFEYGCAGKPVIAPDTVPVRDVMENGTDGLLVKPGKN
                     ELLSALLQMMNDPEKTSGMARHFREKIVSTCTWKRAAEKALLSL"
BASE COUNT          614 a          806 c          662 g          629 t
ORIGIN      
        1 taagcgaaga gccgacatcc acccactcgt agcggtcgac attccccagc atgaaatcat
       61 tccggttggt tcctttctgg ctgaggatga tcgcgcgctg cgcatagcgg ctgtcgttga
      121 caaagagcat tcctccctct ccggcggtta tgttcttggt ttcgtggaac gaaagcgcgc
      181 cgaatgcacc gatcgttcct aaccgctctt tcttccacct cgcatcaatg gcgtgcgcgg
      241 catcttccac cagcgggatc ctatattttt ccgaaaccgc tttcagcttt tccatatcgc
      301 acgcgacacc ggaataatga acacagacaa tcgccttcgt acgcccggtg atcagcggtt
      361 caattgcatc tgcatcgatg ttgggaaaac cgggctggct gtcagcgaaa acaatccgcg
      421 ctccccgcag cacaaaagca ttgaccgtgg aaacatacgc gaacgaaggc gcgatcactt
      481 catctccttc acgaatgttc agcaggcagg cggccagctc gagcgccgcg gtgcatgaag
      541 tcgtcaggat gttgtgcggc acgccgtagt tttcgcggaa aaaagacatg cacatacccg
      601 tgaattttcc gcccgaggaa atatgtcccg aagcgacggc atggcgcata ttgcgcagct
      661 cgcgccccac gatcagcggt ttattgaagg gaatgtgcgg gatcttatcc atgaatgccg
      721 ttaactaaag tacatctccg cttccctgta agcaaacgcg gctgatcttt tctatcgtca
      781 gcccggccac cttttcccag aggaaattat tctccacgtg cttcaccgct cctgcttttt
      841 ttgctgcgag cgcatccggc gaaagcgtga tcgctttttc caatgcacca gccagctgcg
      901 ccacgtcacc tggactgatc agccagccgt tgctttcatt caccatttca gaggtcgcgc
      961 cggtatccgt tgcaatgact gcaaggcccg aagccagcgc ctccatcacg acattcggca
     1021 ttccttccga aaagctcggg cagacaagca catcggcatc gcggagaata tttttcagcg
     1081 cagcggaatc ttttatttcc ccgtgatagg taagccgcgc atcctttacc cacttcttcg
     1141 gcggaatatc tcccacgaag ctgaaggcga attccatcgt ccccggtaat tgtgcgatgg
     1201 cttcatgcag ctcgcacaat cctttccggc gttcattcct tccgacgaaa acaaaacggc
     1261 gcggagcatg cacttccggc gtgcccgtaa aaagccattc gcgtgaaatg ccggtcggga
     1321 tttcgatgat cttttctgcg cccgcaccaa gactgcggat gagggctgtt atttttccgc
     1381 catacgaaaa aaggtaatcg gcgtgcctca cgttaaacag cacaggagaa cgcagcagga
     1441 acctgctctg cagccaggaa acaaaaccgg gctgcggctg gaacatttca tacccgtgaa
     1501 aattcagccc gacaggcggc agcttttctc ccttgctttt ccggttgagc agctcccatc
     1561 cggcaaagcc tttcacgtaa atgaaatcag gcagcgtttc gctgttcagc tttttccaca
     1621 cgcggcagga atattcatac gactcgcgga tgtaatggcc cggcatgcgc ccgaaatccg
     1681 ggaaaggaat aacgaatgaa cgcaggttct ttttttcttc cccggtaaac aggtcaaacg
     1741 caccttcctt ttttccgccc ggtattgtat ggtaaaggtc gacctgcaca cctgcagccg
     1801 caaggtattt ggcgaggtag aacgaatgcc gctgcattcc accaatcacg tggggaaaaa
     1861 ttccatcggt aagcagcgcg atcctcatgc gtcaaaaacc agtttgtgcg attgcattgg
     1921 ccttacaaag ataacagggc cttttccgcc gcgcgtttcc acgtgcaggt gctcacgatc
     1981 ttttcccgga aatgcctcgc catgccggaa gtcttttccg ggtcgttcat catctgcagc
     2041 agggcggaaa gcagctcgtt ctttccgggc ttcacgagca acccgtccgt tccgttctcc
     2101 atgacgtcgc ggaccggaac cgtatcgggt gcgatcaccg gtttcccggc acaaccgtat
     2161 tcgaaaattt tcacaggcga tccataccag ttcgaatccg ccatgacggt aatgtccatc
     2221 gcagcaatgt gatcgaaaat atccgcatgc ggaacagaac ccgtgaaaac gacggaatcg
     2281 ccgcagccga gcgccgacgc cagctctttc agctccggga taagataccc gtcgccgaca
     2341 ataaacaatc ctgtttcgct atgctgccga tgcaattctg caaatgcgcg gatgagaata
     2401 tcaacgccat ggtacgggaa tatcgaaccg acgaaaccga tcactgtttt ttttcccgga
     2461 aaatacttcc ggcgcagctg ttccgctttt gcgggcgaaa cgttaacagc tccgggatcg
     2521 acgcaattcg gcgtgacggt tattttttcc tttttcacgc cgtaccgttc cgccaggtag
     2581 ccggccagcg cagaagaaac ggtgatcaca cgcgccgcat cgtccagcaa ttcgttcatc
     2641 cttttctttc ctgctgcggc cagcatcgat cctgcttttt caaatccccg cacttcttcc
     2701 tcgaagggag c
//
LOCUS       DASTSF010000069         3346 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1295888,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000069 DASTSF010000000
VERSION     DASTSF010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3346
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1295888"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..614
                     /locus_tag="VFU15_02070"
     CDS             <1..614
                     /locus_tag="VFU15_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009577987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEU4716583.1"
                     /translation="FGAELEIIPGDGGKITAALIDKLVARARELSENPNTFWTDQFNN
                     IDNRNAYHAMAREIMNELGTGIDEFIMGVGTGGSFSGNAEIFKKEIPGIRCIAIEPAN
                     VRALSGGDISGKHKLEGIGSGFVPAITRLDLADEIIAVTDEDAYDTARKLARAEGIFG
                     GTTSGANVWAALQRAKELGPGKKIVTIIVDSGLKYLSGDLYCD"
     gene            625..1332
                     /locus_tag="VFU15_02075"
     CDS             625..1332
                     /locus_tag="VFU15_02075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716584.1"
                     /translation="MLIPSPLFGPYKKVSAEIKKLYTAVLKGKTPEDYFSQFTLIDSD
                     RHPESFFWLDRAWAGLGGADHFVLSPVFEPMFFKDKRITAENRWTIKRLLMNYFARSP
                     WGTLVLNANQGYIWTDSELVPRGVSYSICPDSAATILGSTCAHWDEFLAEGNRFFRGG
                     YDAKSFPGSYPLCYFVLAVCGFDTSEFPVEYFEAIPAPPPAGNVKLICEKYDIVNRLK
                     TDENFREKARRGLLAKK"
     gene            1644..3332
                     /locus_tag="VFU15_02080"
     CDS             1644..3332
                     /locus_tag="VFU15_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010665243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GNAT family
                     N-acetyltransferase/carbon-nitrogen hydrolase family
                     protein"
                     /protein_id="HEU4716585.1"
                     /translation="MQRTDRIKMRKSSSRKKIRTVEVRHLRLDDYMGLRDSMHEAYKN
                     WHGSVWTENNIRGLLDVFPEGQVVVLVNGKVVGCALSIVVNYADFGDDHTYRDITGNY
                     TFRTHNRKKGDVLYGIEVFIHPDYRGMRLARRLYDARKELCEKLNLKSIVFGGRIPNF
                     SEHSAEMTPKEYINKVKMKEIYDPVLSFQLSNEFHVKKLLVNYMPGDLESKEYATLME
                     WNNVMYQEDRGTLKQRKSIVRVGLVQWKMRSFRDLHSIYEQIEFFIDSVAGYTADFIL
                     FPELFNAPLMADFNHQSEAEAIRSLADFTEPLRDKFIDFAITYNVNIITGSMPYIKDG
                     QLFNVGYLCRRDGSIERYSKIHVTPNEVSAWGIVGGNKLKVYDTDAGKIGILVCYDVE
                     FPELARLLALDGMEILFVPFLTDTQNSFTRVRSCARARAIENECYVAIAGGIGNLPKV
                     HNMDIQFAQSAVFTPADFAFPTNGIKAETTPNTEMVLIADLDLDLIKKLRSGGSVRNL
                     EDRRTDIYELKRLKPSGRYELLKEYADEKPRFHILNPGILGDQPLNGAGKKQEH"
BASE COUNT          842 a          895 c          882 g          727 t
ORIGIN      
        1 cgttcggcgc ggaactcgag atcattcccg gcgacggcgg aaaaatcacc gcggcgctca
       61 tcgacaagct tgtggcgcgt gcgcgtgaac tgagtgaaaa tcccaataca ttctggaccg
      121 atcagttcaa caatattgac aaccgcaatg cgtatcatgc gatggcgcgg gaaataatga
      181 acgagctcgg aaccggcatc gacgaattca tcatgggcgt gggcaccggc ggaagttttt
      241 caggcaacgc tgaaattttc aagaaagaaa ttccgggcat acgctgcatc gcgatcgaac
      301 ctgcgaatgt acgtgcgctt tccggcgggg atatttccgg taagcacaag ctggagggaa
      361 tcggttccgg ttttgtaccg gcgatcacgc ggcttgatct cgcggatgaa attattgccg
      421 tcaccgatga agatgcgtac gatacggcgc gaaagctggc gcgcgccgaa ggaattttcg
      481 gaggaaccac atccggcgcc aacgtctggg cggcattgca gcgtgcgaaa gaactggggc
      541 ccgggaaaaa aatcgtaacg atcatcgtcg attccgggct gaaatatctt tccggggatc
      601 tctactgcga ttaaccggct gaacatgctt atcccctccc cgctcttcgg tccgtacaaa
      661 aaagtttcgg cggaaataaa aaagctctac accgcggtgc tgaaaggtaa aacgccggaa
      721 gattattttt cacagttcac actgatcgat tcggacaggc atcccgaaag ttttttctgg
      781 ctcgaccgcg cttgggccgg tttgggcggc gcggaccact tcgtgctgag tcccgtgttc
      841 gagccgatgt ttttcaagga caagcgcatc acggcggaga accgctggac gatcaagcgc
      901 ctgctgatga attattttgc gcgatcacca tggggaacgc tcgtgcttaa cgcgaaccag
      961 ggatacatct ggacggattc agagcttgtt ccgcgcggag tttcctattc catctgtccc
     1021 gacagcgccg caacgatcct cggttcgacc tgcgcgcact gggacgaatt cctggcggaa
     1081 ggaaaccgtt ttttccgcgg cggttacgac gcgaaatcat ttcccggatc atacccgctt
     1141 tgctacttcg ttcttgctgt atgcggtttc gacacctccg aattcccggt tgaatatttc
     1201 gaagccatac ctgctccgcc accggccggc aacgtgaaac tgatctgcga aaaatatgac
     1261 atcgtgaacc ggctgaaaac cgacgaaaat ttccgcgaaa aagcgcgaag gggtttgctt
     1321 gcgaaaaaat agcgcctgta aaagatcatc gtttgcggat ccttccggtt ctctcccctg
     1381 ttttacttca ggcacacatt ctctcacggg cgcagttcac tgccaatgat cctcgtgacc
     1441 tgctgcggca tttttcagcc ttgcggcttg ttccccagta acattccccg cttcatcttc
     1501 tttccgtttg ttcaaactgt ttttccgtaa cagatatcct gccaaccatc gaaaaaaaaa
     1561 gtcgttcacc cttcttcctg cttaatttta aaaggcatga aaagcaatat tgttaacgga
     1621 tatcttattt aaaatttaca ggtatgcaac ggacagacag gatcaagatg cggaaaagca
     1681 gttcgcgaaa aaaaatacgc acggttgaag tacgtcacct ccgcctcgat gactacatgg
     1741 gactgcgtga ttccatgcat gaagcgtaca aaaactggca cgggtcggtg tggaccgaaa
     1801 ataacattcg cgggctgctc gatgtttttc ccgaaggaca ggttgtcgtc ctggtgaatg
     1861 gaaaagtcgt ggggtgcgcg ctttcgatcg ttgtcaatta cgcggatttc ggcgacgatc
     1921 acacataccg cgacatcacc ggaaattata cgttccgcac gcacaaccgg aaaaagggcg
     1981 atgtccttta cggcatcgaa gtattcattc acccggatta ccgcgggatg cgccttgcac
     2041 gcagacttta cgacgcacgc aaggaactct gcgaaaagct gaacctgaaa tcgatcgtct
     2101 tcggcggaag gattcccaac ttcagcgaac attcggcaga gatgacaccg aaagaataca
     2161 tcaacaaagt gaagatgaaa gagatctacg atcctgttct ttctttccag ctgtcgaatg
     2221 aattccacgt gaaaaagctg ctcgtgaatt acatgcccgg cgacctcgag tcgaaagaat
     2281 acgccacgct gatggaatgg aacaacgtga tgtaccagga agaccgggga acgctgaaac
     2341 agcggaaaag catcgtgcgc gtgggactgg tgcaatggaa aatgcgctcc ttccgcgacc
     2401 tccattccat ttacgagcag atcgaattct tcatcgattc ggtggcgggt tacacggcgg
     2461 atttcattct tttccccgag ctgttcaacg caccgctcat ggctgatttc aaccaccagt
     2521 cggaagcgga agccatacgc agcctggcgg atttcaccga accgctccgc gacaagttca
     2581 tcgatttcgc catcacttac aacgtcaaca tcatcacggg aagcatgccg tatattaaag
     2641 acgggcagtt gttcaatgtc gggtacctgt gcaggcggga cggatcgatc gagcggtatt
     2701 cgaagatcca cgtgacgccg aacgaagtga gcgcatgggg catcgtgggc ggcaacaagc
     2761 tgaaagtgta cgacaccgac gccgggaaga tcggcatact cgtctgctac gacgtggaat
     2821 ttccggagct ggcacgcctg ctcgcgctgg acggaatgga aatacttttc gttcctttcc
     2881 tcaccgacac gcagaattcc ttcacgcgcg tacgcagctg cgcgcgggcg cgggcgatcg
     2941 agaacgaatg ttatgtggcg atcgccggcg gcatcggcaa ccttccgaaa gtgcacaaca
     3001 tggacatcca gttcgcgcag tcagctgttt tcacacccgc cgatttcgct tttcccacca
     3061 atggcatcaa agcggaaacg acaccaaaca cggaaatggt gctcatcgcc gacctcgatc
     3121 tcgacctgat caaaaagctg cgttcaggcg gaagcgtgcg gaacctcgaa gaccgccgga
     3181 cggatattta cgagctgaag aggctcaaac cgagcggacg ttacgagctc ctgaaagaat
     3241 atgcggacga gaagcccagg ttccacatcc tgaatcccgg catactcggc gatcagccgc
     3301 tcaacggagc cggtaaaaag caggagcact gagaatgaaa ttccgg
//
LOCUS       DASTSF010000070         3776 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1519520,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000070 DASTSF010000000
VERSION     DASTSF010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3776
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1519520"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            130..2313
                     /locus_tag="VFU15_02085"
     CDS             130..2313
                     /locus_tag="VFU15_02085"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEU4716586.1"
                     /translation="MKPPLLFIPCSDAGSADASVNLFYVLHSPQLDYYFSDRGYSIVQ
                     KDAKGKAENRTDVFFRNGKRIIPEGESVSPVKLNTCSAGRSKKETAFTQLRYRNFSGA
                     SDLVFRIDGDSLEKTITHPGFYSFASSFEVDGAVLLSDSSCNDFHSAAGDIREKMAAG
                     CSPVPVNQQRRLPHGWMLTTNARAVAPLQFFSQSLSWMTYIGGTDSDELFGIDLTRDD
                     GVVVCGRTGGTDFPSLPGAMQDTSAGSYDAVILRFDADGNCLWSTYYGGTGFDGAYAL
                     VTLDSCFAICGMTNSTDLPVSGGPQLSNAGSYDGFILLLDDSGQAVKSTYIGGTGADQ
                     VFSIAKGRGDTLVIAGSTSSANFPYTSGQFQPACAGLLDAFICVMTDSLVPQWATYYG
                     GSSSEDIHHIIVSPQDEIIFCGGTYSNNFPLTPNAWQGGVISTPDVYLVKFGMDGARH
                     YATCIGGSAGEDANMLAADSAGNFYVTGFTYSFDFPIQGNSFQSMISAQNDAYVAKFD
                     PQGQLSWSTFIGGNGVDIGLCMQRRGEYLYVAGQTESTDFPLSGNSMQVNYGGNGDGF
                     VVKLDTAGNMVAGTYVGGNGVDAVYGISVTSDTNVFCTGDTYSTDLPVTPGAFQSVNN
                     GQGEGFLCRFKMSDQLFSTGTAGDRATEAVSVYPNPSSGLVQVTSSSVITQVTVTDAE
                     GRIVSSQKNNSETVSLDISGFAPGIYWLNIWGENGAHSVVRVVKY"
     gene            complement(2320..2811)
                     /locus_tag="VFU15_02090"
     CDS             complement(2320..2811)
                     /locus_tag="VFU15_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011585656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HEU4716587.1"
                     /translation="MASFDIVSKIDTQTLDNAINSARKEIFGRFDFKGTQSTIDLDKK
                     NLTLQIVTEDEMRLNSIIDIIRHRMVKQQIDSRCIDEGKEHYASGNMIRKDLKIKSGI
                     DKDTARKIIKDIKDSKLKVQAQQMDDQVRVTGKKYDDLQTVISLVRKGNYELPLQFVN
                     MKG"
     gene            complement(2861..>3776)
                     /locus_tag="VFU15_02095"
     CDS             complement(2861..>3776)
                     /locus_tag="VFU15_02095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716588.1"
                     /translation="GLEFPVVILPFASWDFKVQKKEIWITPEDELVPELETALVSTGK
                     NLLKTKIAGRYTTEETRSHLDMLNLLYVAMTRPEKRLYVFTGKVKEIKSPPVNVSQCF
                     AFYLQTLKAEGDCFESGTREKPSHAGPPVSRVISAPAGDPVSWYHRVKIRSLASDFGD
                     GETRTNAAKGKMLHRVLSLLKDRTGADEAIRKAAALGWIPEDDRDYFLREVNGILSIP
                     ELEECFDTTGNFIERKILVPGSGNYRPDRVTIKKEKCIVLDYKTGKALPAHRAQLDGY
                     GDLLKKMGYREIEKKLVYTENGEVISWQ"
BASE COUNT          784 a          956 c         1026 g         1010 t
ORIGIN      
        1 gaatatggtg tcgggtcggc aagcccgaca tcgtgtttta tgaacttcgg tgtaacttta
       61 taagtaatgg caaaacgact tctctttttt ttattcgcct cgttttctgt atccgcttat
      121 tgctccggga tgaaaccacc gttgcttttc atcccgtgca gcgatgcggg cagcgctgat
      181 gcgtccgtca acctgtttta tgtgttgcat tctcctcaac tggattatta tttttcagac
      241 cggggatatt ccattgtgca gaaagatgcg aagggaaaag cggagaatcg tacggatgtt
      301 tttttcagga acggaaaaag aataattccg gaaggagaga gcgtgtcgcc ggtgaagctg
      361 aatacttgtt cagccggaag gagcaaaaaa gaaacggcat tcacacagct gcgctaccgg
      421 aatttcagcg gcgcgtccga tcttgtgttc cgcattgacg gcgattcgct ggaaaaaacg
      481 atcacgcatc ccgggtttta ttcattcgcc tcttcttttg aagtggatgg agcggtgttg
      541 ctttccgatt catcgtgcaa tgattttcat tcagctgcgg gcgatatccg tgaaaaaatg
      601 gctgctggct gttcacctgt tcccgtcaat cagcagcgcc ggcttccgca tggatggatg
      661 ctgacgacaa atgcacgcgc ggtagctccg ctccagtttt tttcgcaatc gctttcatgg
      721 atgacctaca tcggtggaac ggattccgat gaactgttcg gcatcgatct tacgcgcgat
      781 gacggcgtgg ttgtctgtgg ccgcaccggg gggacggatt ttccttcgct gccgggcgcc
      841 atgcaggaca cttccgccgg gagctacgat gcggtcattc tgcgtttcga tgcggacgga
      901 aattgtctct ggtccacgta ttacggtgga acgggtttcg acggcgccta cgcgctcgtc
      961 acgctcgatt cctgcttcgc catttgcgga atgacaaaca gcaccgatct tcccgtttcc
     1021 ggcggaccgc agctttcgaa tgccgggtcg tacgatggtt ttatcctgtt actcgacgat
     1081 tcgggccagg cagtgaaatc gacgtacatc ggcggaacgg gagctgacca ggttttctcg
     1141 atcgcgaaag gaagaggaga cacgctggtg atcgccggtt cgacgagctc cgcgaatttt
     1201 ccttatacat ccgggcaatt ccagccggcg tgcgcaggat tgctggatgc atttatctgc
     1261 gtgatgacgg attcgcttgt tccccaatgg gcgacgtatt acggcggttc cagcagcgag
     1321 gatattcatc acatcattgt ttcgccgcag gatgaaatta ttttctgcgg aggaacgtac
     1381 agcaacaatt tcccgcttac acccaatgcc tggcagggcg gagttatttc cacgccggat
     1441 gtctatctcg tgaaattcgg aatggacgga gcgcggcatt atgccacctg catcggcgga
     1501 tctgcgggag aagatgcgaa catgctggct gcagacagcg ccgggaattt ttatgtcaca
     1561 ggttttacct acagctttga ttttcccatc cagggaaatt cattccagtc gatgataagt
     1621 gcgcagaatg atgcatacgt ggcgaaattc gatccgcagg gacagctcag ctggtcgaca
     1681 ttcattggcg gcaatggcgt cgatatcggg ctctgcatgc agcgccgggg cgaatatctt
     1741 tacgtggccg gtcagaccga aagcactgat tttccgctga gcggaaattc gatgcaggtg
     1801 aattacgggg gcaacgggga cggcttcgtg gtgaaactcg acaccgcggg gaacatggtc
     1861 gccggcactt acgtcggggg caacggcgtg gatgcggttt acgggatttc cgtcacttcc
     1921 gataccaacg ttttctgtac cggcgacacc tacagcacgg atcttcccgt tacgccgggc
     1981 gcattccagt cggtgaataa cgggcagggg gaaggattcc tgtgcaggtt caaaatgagt
     2041 gatcagctgt tctctacggg aacggcggga gaccgggcaa cggaggcagt ttccgtttac
     2101 ccgaaccctt cttccggtct tgtgcaggtg acttcatcct cggttattac acaggtaacc
     2161 gttacagatg cggaaggaag gatcgtgagt tcgcagaaaa ataattcaga aactgtttca
     2221 ctggatattt ccggttttgc accggggatc tactggctga acatttgggg ggagaacgga
     2281 gcccattccg ttgtacgcgt ggtaaaatac tgacgcggtt caacctttca tattgacgaa
     2341 ttgcaacggt aattcatagt tgcctttccg cacgagcgag atcacggtct gcaggtcatc
     2401 gtattttttc cctgtcacac gcacctggtc gtccatctgt tgcgcctgca ctttcagctt
     2461 cgaatccttg atgtctttga tgatcttccg cgccgtgtct ttgtcgatgc cggattttat
     2521 tttcaggtct ttgcggatca tgtttcccga agcgtagtgt tcctttcctt cgtcgatgca
     2581 gcgtgaatcg atctgttgtt tcaccatccg gtggcgtatg atgtcgatga tggaattgag
     2641 acgcatttca tcttccgtta cgatctgcag tgtaagattt ttcttgtcga gatcgatcgt
     2701 gctttgcgtg cctttgaaat cgaagcgccc gaaaatttcc tttcgcgcgg aattgatcgc
     2761 gttgtcgagc gtctgcgtgt cgatctttga aacgatgtcg aagctggcca tgagtaaaag
     2821 ttttaaaata accggttcct gaaaatacaa aacgcctgct ttattgccag gatatcactt
     2881 ccccgttttc cgtgtacact aatttttttt cgatttcccg gtatcccatt tttttcagca
     2941 ggtcgccgta cccgtccagc tgggcgcgat gtgcaggcaa cgcttttcct gttttataat
     3001 cgagcacgat gcatttttct tttttgatgg ttacgcggtc ggggcggtaa ttcccggagc
     3061 cggggacaag aattttccgt tcaataaagt ttccggtcgt atcgaagcat tcttccagct
     3121 ccgggatgga aaggatcccg ttcacctcgc ggagaaaata atcgcgatca tcttccggta
     3181 tccagccgag cgccgccgct ttccggatgg cttcatctgc cccggtgcga tctttcagca
     3241 aagaaagaac acggtgcagc atttttcctt tcgcagcgtt cgttcttgtt tccccgtcgc
     3301 cgaaatcgga agcgagggaa cggatcttca cgcggtggta ccagcttaca gggtcgccgg
     3361 cgggagcgct gatcacgcgc gaaaccggag gacccgcatg cgaaggtttt tccctggttc
     3421 ccgattcgaa acaatcgcct tccgccttca gcgtctgcag gtagaacgcg aagcactgcg
     3481 aaacattcac cggcggtgat ttgatctctt tcaccttccc ggtgaaaacg tacaatcttt
     3541 tttccggccg cgtcattgcc acgtagagca ggttcagcat gtcgaggtgt gatctcgttt
     3601 cttccgtcgt gtaccggccg gcgatctttg ttttgagaag gtttttcccg gtggagacaa
     3661 gcgccgtttc cagctccgga accagctcgt cttccggcgt gatccagatc tctttcttct
     3721 gcaccttgaa atcccagctg gcgaacggaa ggatcactac aggaaattcc aggcct
//
LOCUS       DASTSF010000071         2699 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_398672,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000071 DASTSF010000000
VERSION     DASTSF010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2699
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_398672"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            257..919
                     /locus_tag="VFU15_02100"
     CDS             257..919
                     /locus_tag="VFU15_02100"
                     /inference="COORDINATES: protein motif:HMM:NF025488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4290 domain-containing protein"
                     /protein_id="HEU4716589.1"
                     /translation="MTDQSETENATGMEYNTQQPVMAIAEYGRNVHRMIAYCLTLTDR
                     EERTRCAKTIVRVMAILNPTLREYADYEHKLWDHLHIMSGYNLDVDSPFAKPEKAALD
                     SPPEIVPYPQSNIRFKHYGKILEDLIKKAMAEEDPKNREIFTDQLAQLMKRQYLNWNR
                     DSVNDQLIVEQLESLSGGKLKVSEQFRFLHTSEILPKNAIPQNQMQHHTGKKKMMKKK
                     KR"
     gene            1100..2407
                     /gene="murA"
                     /locus_tag="VFU15_02105"
     CDS             1100..2407
                     /gene="murA"
                     /locus_tag="VFU15_02105"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013684835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEU4716590.1"
                     /translation="MSYFEITGGKKLKGELVPQGAKNEALQILSAVLLTPEKMTISNI
                     PDIVDVNKLIDLLRDLGVKVEKTGHEQYTFQADDIDIDYINSDKFRNKGGALRGSIMI
                     VGPLLTRFGKGYIPKPGGDKIGRRRLDTHFIGFENLGAKFTYDSANEFYRVEAKRLKG
                     AHMLLDEASVTGTANIVMAATMAEGVTTIYNAACEPYLQQLCKMLNRMGAKISGVGSN
                     LLTIEGVGYLGGTEHRMLPDMIEVGSFIGLAAMTQSEILIKNVSFENLGVIPNTFSRL
                     GIMMERRGEDLFIPGQDIYEIDTFIDGSILTIADAPWPGFTPDLLSIVLVVATQAKGS
                     VLIHQKMFESRLFFVDKLIDMGAQIILCDPHRATVIGLGREHHLRGVTMTSPDIRAGV
                     SLLIAALSAKGKSIIHNIEQIDRGYQDIDNRLRKLGAEIARKN"
     gene            2496..>2699
                     /locus_tag="VFU15_02110"
     CDS             2496..>2699
                     /locus_tag="VFU15_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEU4716591.1"
                     /translation="MKRILFLFAFAVITAAATAQTGNVVGINVGNIAPDISMKNPQDS
                     TLTLSSLRGKMVLIDFWASWCGPC"
BASE COUNT          694 a          811 c          664 g          530 t
ORIGIN      
        1 gtacacgaag cttgtcacaa agtgcacgga ggaaaacagg aaacggtcaa cgctgttcag
       61 gcccggacgc cattcaaacc cgaaactgaa aagttctttt taacacagag aaccacggag
      121 gccgtttcac agaggaaaca cggaggaccc gaaactgaaa acccggaacc gcaaacccgg
      181 atgaaggcgc caatccgatc ccttttacta tctttgtggc tctccccgga gagatttccc
      241 taacaacatc ttaagaatga cagaccagtc agaaaccgag aatgccaccg gcatggaata
      301 caatacgcag caacccgtca tggcgattgc cgaatacgga cgcaatgttc accggatgat
      361 cgcgtattgc ctcacgctta cagacagaga agaacggacg cgctgcgcga aaacgatcgt
      421 gcgcgtgatg gcgatactta atcctaccct ccgcgaatac gccgattacg agcacaagct
      481 gtgggatcac ctgcacatca tgtccggcta caacctcgac gtcgattctc cgttcgcaaa
      541 gccggaaaaa gcagcgctgg attcacctcc cgagatcgtg ccttacccgc agagcaacat
      601 ccgcttcaag cattacggca agatcctcga ggaccttatc aaaaaagcga tggcggaaga
      661 agatccgaag aaccgcgaga tcttcaccga ccagctcgcg cagctgatga aacgccagta
      721 cctcaactgg aaccgcgatt ccgtcaacga ccagctcatc gtcgagcagc ttgaaagcct
      781 ttccggcggc aagctgaaag tgagcgagca gttccgcttc cttcacacaa gcgaaattct
      841 tcccaaaaat gccatcccgc agaaccagat gcagcatcat accgggaaga aaaaaatgat
      901 gaagaagaaa aaacgctgac ccgtcccggt cacaggtttt gtgttccggt ctttcgcttt
      961 gcactttgaa cccggaacct gaaagcagga acgacgaata cgtagttcac atttcccggt
     1021 taaaacattt tcccttccct cctgatttgc cttcgcttta ggactgaact ttattcacct
     1081 aaagtcaaac gcgaatctca tgagctattt cgaaatcacc ggtggaaaaa agctgaaagg
     1141 cgagctggtg ccccagggcg cgaagaacga agcgctgcag atactttcgg ccgtgctgct
     1201 cacgccggaa aaaatgacga tcagcaatat tcccgacatc gtcgatgtga acaagctgat
     1261 cgacctgctc cgcgacctcg gcgtgaaagt cgaaaaaacc ggccacgagc agtatacgtt
     1321 ccaggccgac gacatcgaca tcgactacat caactccgac aagttccgca acaagggcgg
     1381 cgcgctccgc ggctccatca tgatcgtcgg tccgctgctc acacgtttcg gcaaaggcta
     1441 cattcccaaa cccggcggcg acaagatcgg gcggcgcagg ctcgacacgc atttcatcgg
     1501 ttttgaaaac ctcggcgcaa aattcacata cgattccgcg aatgaatttt accgcgttga
     1561 agcgaaacgg ctcaagggcg cgcacatgct gctcgatgaa gcatccgtta cgggaacggc
     1621 gaacatcgtc atggccgcca ctatggccga aggcgtcacg acgatctaca acgcagcctg
     1681 cgaaccttac ctccagcagc tctgcaaaat gctcaaccgc atgggcgcaa aaatatccgg
     1741 cgtcggctcc aatctcctca ccatcgaagg cgtcggctac ctcggcggaa ccgagcaccg
     1801 catgcttccc gacatgatcg aggtgggcag cttcatcggt ctggccgcca tgacgcaatc
     1861 cgagatcctc atcaaaaatg tttccttcga aaatctcggc gttatcccca atacgttcag
     1921 ccgtctcggc atcatgatgg aacgccgggg cgaagatctt ttcattcccg ggcaggacat
     1981 ttacgagatc gacacgttca tcgacggctc cattctcacg atcgccgatg cgccatggcc
     2041 cgggttcacg cccgacctgc tcagcattgt gctcgtcgtc gcgacgcagg cgaaaggaag
     2101 cgtgctcatc caccagaaaa tgttcgaaag ccgcctgttc ttcgtcgaca aactcatcga
     2161 catgggcgcg cagatcatcc tctgcgatcc gcaccgcgcc accgtcatcg ggctgggacg
     2221 cgagcatcac ctgcgcggcg tgacaatgac ctccccggat atccgtgccg gtgtttccct
     2281 gctcatcgct gcgctttcgg ccaaaggaaa gagcatcatt cacaacatcg agcagatcga
     2341 ccgcggttac caggacatcg acaaccgcct gcgcaagctt ggtgcggaaa ttgcaagaaa
     2401 gaactgacgg aacaccgggc gggagtcgtt taccaacaaa cgttaacggc ttccggcgcc
     2461 ttttttccgc ctgaattctt tctaacttta gcagcatgaa acgcattctt tttctcttcg
     2521 cttttgccgt gataacggct gccgccacgg cgcagacggg taatgtcgtc ggcatcaatg
     2581 tcgggaacat cgcccccgat atttccatga agaatccgca ggattccacg ctcacgcttt
     2641 cctcactgcg gggaaaaatg gtcctgatcg atttctgggc atcgtggtgc ggtccctgc
//
LOCUS       DASTSF010000072         3177 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1739566,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000072 DASTSF010000000
VERSION     DASTSF010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3177
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1739566"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..473)
                     /gene="trmB"
                     /locus_tag="VFU15_02115"
     CDS             complement(<1..473)
                     /gene="trmB"
                     /locus_tag="VFU15_02115"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010266164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEU4716592.1"
                     /translation="MYKNKLTRFAEMKSMANVVQYTFRDLEKGIHPLRGKWKQDFFRN
                     EKPIVLELGCGKGEYTVGMATRFPGKNFIGVDIKGARMHRGASQAQEAGLHNAAFLRT
                     RIDFINALFAENEVSEIWITFPDPQKEKPKKRLTSPGFLVRYAKLLTPGGIIHLKT"
     gene            802..1869
                     /locus_tag="VFU15_02120"
     CDS             802..1869
                     /locus_tag="VFU15_02120"
                     /inference="COORDINATES: protein motif:HMM:NF024918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HEU4716593.1"
                     /translation="MKKRILISPLDWGLGHATRIVPVMRELEKQGAEVIIAAGGRPYY
                     FLKKAFPGNEIIEAGGYDITYPRNGNLGLHLLKKLPGMARKVKEEKKLAEKIAAEKNI
                     DIIISDNRFAFFSERTHNVYITHQLNVKTPKGFGIANAIHRKYFGKFDEVWVPDFPGA
                     ENLSGSLGHGTEHKKNTFYVGPLSRFSERISSVQEKKWQVIALLSGPEPQRTMFEEKI
                     IEQAADLNGRVLIVRGTPEEKEEAKSAESTVVLKNHLADEELVAEISSAEVVLSRGGY
                     TTLCDLFYLGVPFVAVPTPGQTEQEYLAALHSANGNLFTMTQEKFSLREAFEKGKNLE
                     PISIHDNERRLERAVKELLQK"
     gene            complement(1889..2932)
                     /locus_tag="VFU15_02125"
     CDS             complement(1889..2932)
                     /locus_tag="VFU15_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU4716594.1"
                     /translation="MKQPSLRLLAFRTALISGAIAAIACFIPVALSSLPAAFLSAAIS
                     LVVIGVLVYFLFSHYSRKFINAPLRSLYKAIYNLKIDDTQTLDIPVEDEPLLQMQREV
                     TEWMKNRSAEIEDLKKLEIYRREFLGNVSHELKTPIFNIQGYVETLLDGGLEDPGINR
                     NYLQRAEKSVERMINIIDDLESISRLESGELIIERYPFDIYELVMEIYYAFEMKAGSK
                     GIKLAFRDPYKPVYVFADKDRIRQVITNLVSNSIKYGKDGGQTETRFYQVDDNILIEI
                     ADNGIGIAREHLPRLFERFYRVDKGRSREQGGTGLGLAIVKHILEAHGQGINVRSSEG
                     IGSTFSFTLPKPK"
     gene            complement(3027..>3177)
                     /locus_tag="VFU15_02130"
     CDS             complement(3027..>3177)
                     /locus_tag="VFU15_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEU4716595.1"
                     /translation="AREEILNRVWGDDVVVGDRTIDVHIRKLREKIGEDFIKTVKGVG
                     YKFEF"
BASE COUNT          740 a          815 c          904 g          718 t
ORIGIN      
        1 gtcttcaggt gaatgatgcc gcccggcgtg agcagcttcg cgtagcggac aagaaatccc
       61 ggcgaggtga gacgcttctt cggtttttct ttctgcggat ccgggaacgt gatccagatc
      121 tcgctcactt cattttccgc gaacagcgcg ttgatgaaat cgatgcgcgt acgcaggaat
      181 gcggcattgt gcaggccggc ttcctgtgcc tgcgaggcgc cccggtgcat gcgcgcgccc
      241 ttgatgtcga cgccgatgaa atttttcccc gggaagcgcg tcgccatgcc gacggtgtat
      301 tcgcctttcc cgcagccaag ctcgagcacg atgggttttt cattgcggaa aaaatcctgc
      361 ttccatttcc cgcgcagcgg gtgaatccct ttttccaggt cgcggaacgt gtactggacc
      421 acgttcgcca tcgacttcat ttccgcaaaa cgcgtaagct tgttcttgta catgaggacg
      481 ggcgcgaaga tacgaagcgg cgggaggtat tgagaagggt ccggcgatgg ttttattccg
      541 cagaaagacc gcccggcgag cgtttgtttt ctgtgatgtg tgacgctgca cgttttactt
      601 tttctgcagg tttttccgga aaaaagcggc aaaaaaaaac atgaccggcg cgacatgtga
      661 gcaattctgc gctgcctgtg aagaacaggg tttgttcctg gaacttaaaa aaaacaagca
      721 gcacttgctc acagcctgcc gccacccgtc tttccacctt tgtatcttcg ttccggcccg
      781 gatcatgccg cgcttccttt catgaaaaaa cgcattctca tttctccgct cgactggggc
      841 ctcggtcacg ccacgcgcat cgtgcccgtg atgcgcgaac tggaaaagca gggcgcggaa
      901 gtgatcatcg ccgccggcgg aaggccgtat tattttctga aaaaggcctt tccggggaac
      961 gagatcatcg aagcgggagg atacgacatc acctatccgc gcaatggaaa tctcgggctg
     1021 catttgctga aaaaactgcc gggaatggcg cgaaaagtga aagaagaaaa aaagctggcg
     1081 gaaaagatcg cggcggaaaa aaatatcgac atcattattt ccgacaaccg cttcgctttt
     1141 ttttcagaac gcacgcataa cgtttacatc acgcaccagc tgaatgtgaa aacgccgaag
     1201 ggattcggga tcgccaatgc catccaccgg aagtatttcg gaaaattcga cgaggtgtgg
     1261 gtgccggatt tccccggcgc tgaaaatctt tccggctcgc tcggtcacgg gacggaacac
     1321 aaaaaaaata ccttttacgt cggaccgctg tcgcgttttt cagaacggat ttcttctgtg
     1381 caggaaaaaa agtggcaggt gatcgcattg ctcagcgggc ccgagccgca gcggaccatg
     1441 ttcgaggaaa aaataattga gcaggccgcc gacctgaacg ggcgcgtgct catcgtccgc
     1501 ggaacgccgg aagaaaaaga agaagcaaag agcgcggaat caaccgtcgt tttgaaaaac
     1561 caccttgcgg acgaagagct tgtcgcggaa atttcttctg cggaagtggt gctttcacgc
     1621 gggggctaca cgacgctctg cgatcttttt tacctcggcg ttcccttcgt tgcggttccc
     1681 acgccggggc agacggaaca ggaatatctc gcggcgctgc acagcgcaaa cggaaatctt
     1741 ttcacgatga cgcaggaaaa attctcgctg cgtgaagcgt ttgaaaaagg aaaaaacctg
     1801 gagccgatct ccattcacga caatgaaagg cggctggagc gtgcggtgaa agaattgctg
     1861 cagaagtagt ccgtggaatt ccgcggtatc acttcggttt gggcagcgtg aacgagaacg
     1921 tcgagccgat gccttcgctg ctgcggacgt taatgccctg cccgtgtgct tcgagaatgt
     1981 gtttcacgat ggcgagtccg agtccggttc cgccctgctc gcgtgaacgt cctttgtcga
     2041 cgcggtaaaa acgttcgaac aggcgcggca ggtgctcgcg cgcaatgccg atgccattat
     2101 ccgcgatctc gatgaggatg ttgtcgtcca cctggtaaaa gcgcgtttcc gtctggcctc
     2161 cgtcttttcc gtatttgatg gaatttgaaa cgaggttggt gatcacctgc cggatgcggt
     2221 ctttgtcggc gaacacgtaa acgggtttgt acggatcgcg gaaagcgagc tttattcctt
     2281 tcgatcccgc tttcatttcg aacgcataat agatttccat caccagctcg taaatgtcga
     2341 acgggtaacg ctcgatgatc agctcaccgg attcgagtcg ggaaatgctc tcgagatcat
     2401 cgatgatgtt gatcatgcgt tccacgcttt tttccgcgcg ctgcaggtaa ttgcggttga
     2461 tgccgggatc ttcgagtccg ccgtcgagca gcgtttccac gtaaccctgg atgttgaaga
     2521 tcggcgtctt cagttcgtgt gaaacattgc cgaggaattc gcgccggtag atctcgagct
     2581 ttttcaggtc ttcgatctcg gccgaacggt tcttcatcca ttccgtcact tcgcgctgca
     2641 tctgcagcag cggttcgtct tccacgggaa tgtcgagcgt ctgcgtatcg tcgatcttga
     2701 ggttgtaaat ggctttgtag agcgagcgga gcggggcgtt gatgaatttc cggctgtagt
     2761 gtgaaaaaag aaaatagaca agcacaccga tgacgacgag cgaaatcgct gcggaaagga
     2821 atgccgcggg cagtgaagaa agcgcaacag gaatgaaaca ggcgatcgcc gcgatcgcgc
     2881 cggaaataag cgcggtgcgg aacgcaagga ggcgaaggga gggttgtttc atgcagcaaa
     2941 gttaatcgga aacacgagca gggaaggcgc gcacgggggc tttgccccaa aggcaaacta
     3001 acgataagaa aaaagaaaca ggaaaatcag aattcgaact tgtagccgac tcccttgact
     3061 gttttgatga agtcttcccc gatcttttca cggagcttgc ggatgtgcac atcgatggtg
     3121 cggtcaccca ctacgacatc atctccccag acccggttga gaatttcttc gcgggca
//
LOCUS       DASTSF010000073         8432 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_397501,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000073 DASTSF010000000
VERSION     DASTSF010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 8432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8432
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_397501"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..428
                     /locus_tag="VFU15_02135"
     CDS             <1..428
                     /locus_tag="VFU15_02135"
                     /inference="COORDINATES: protein motif:HMM:NF024498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEU4716596.1"
                     /translation="NLHEAECYAKSQGKPLFIDFTGYACVNCRKMEDNIWPDSRVYQF
                     LSEKYVVVSLYVDDKKELPAEQQVTDAQGRRVKTYGNVWSNIEADYYQQNSQPLYVLI
                     DDDGKLLAPPRGYTPDAAKYADYLQKGLDVFEKRKAGTK"
     gene            complement(432..1160)
                     /gene="lptB"
                     /locus_tag="VFU15_02140"
     CDS             complement(432..1160)
                     /gene="lptB"
                     /locus_tag="VFU15_02140"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006989970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HEU4716597.1"
                     /translation="MILGAENIVKSYKNRQVVKGVSVNVKRGEIVGLLGPNGAGKTTS
                     FYMIVGMIKPNSGRIYLDDRDITREPMYKRAQLGIGYLAQEASVFRNLTVEDNIRAVL
                     EMTKLTKGEQVNKLESLLDEFGLQHIRKNMGNALSGGERRRTEIARCLATSPGFILLD
                     EPFAGVDPIAVEDIQGVVASLKKKNIGILITDHNVHETLSITDRAYLLFEGTILKAGT
                     AEELAADEQVRRVYLGKNFELRRH"
     gene            complement(1242..3689)
                     /locus_tag="VFU15_02145"
     CDS             complement(1242..3689)
                     /locus_tag="VFU15_02145"
                     /inference="COORDINATES: protein motif:HMM:NF039501.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5686 family protein"
                     /protein_id="HEU4716598.1"
                     /translation="MRKILSVLVSFFLFVSAFAQVKITGKVYNVATNETMPFVSIIVV
                     GTTTGTVSDIDGNFSLTAPAGADSLRAVYVGYLPSTVAIRKGVSPQVLNIPMKVAGEM
                     DIVDIHPGENPAVTLLKKVYAHKDQNDKRRLDCYQYEVYNKLEFDLNNITPKFQQSKV
                     MKPVKFIFDDIDSTNPSEKPNLPIFFSESISDFYYKKSPECHKEVIKGSQVSGIQDAS
                     VSQFTGDMYQDVDIYKNNILVFGKLFVSPISNNGTLFYHFYLIDSTVIDGHWCYQIQF
                     KPKRKQELLFYGNMWIADTAFAVKRIEMNIADDANINYVNSFYVIQEYDNASGAWMMT
                     KERVIGDFNMTNKQMGFYGRKTTSYRDILVNQCKDDDFYTRTDNLIVQPGAESRDSAF
                     WANARHDSLTRTERQIYARVDSVQHLPIYKTWETVAITAYTGYKTLGDFEYGPWYKTV
                     SYNRIEGTRLRVGGRTSNDFSKWVELNGYVAYGCKDEEFKYGAGFKTFITKKPRQLTG
                     VNYKNDFEILGLSNNAFSNDNIMATIFRRTPFTNYTRVEQYEWWYERDIFKGLNMHLS
                     LVNRNMYPLGDGYIHPLSDGGFDTLPRISTSELRLNIRFAFNEKYVEGVFSRTSVGTL
                     WPVFQVQYVSGFKGVFGSQYAYQRLTINCDDRIRINPIGYLNYTFEVGKTWGRVPFPL
                     MILHPGNETYVYDGTAYNMMNYFEFASDQYASAILIHHFDGFFFNKIPLIRKLKWREV
                     LSFRALYGSVSKANRNEILFPSTLYSLDHGPYMELGAGIENIFRFFRVDCFWRLNYLD
                     HPNVAPVGVRIGLQIIF"
     gene            complement(3800..4144)
                     /locus_tag="VFU15_02150"
     CDS             complement(3800..4144)
                     /locus_tag="VFU15_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018476355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEU4716599.1"
                     /translation="MSSVSEFNTYREKMNGKILAADNLVLKRLFNLDTNTYQDGALST
                     KTKEMLGLVASMVLRCDDCVKYHLGKCHEAGVTKEELFEVFAVANIVGGTIVIPHTRR
                     AVEYWEELTAGR"
     gene            complement(4217..5059)
                     /gene="tatC"
                     /locus_tag="VFU15_02155"
     CDS             complement(4217..5059)
                     /gene="tatC"
                     /locus_tag="VFU15_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019945961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEU4716600.1"
                     /translation="MRAFMGLFSTNKNKAGEMSFLGHVEALRWHLVRSAIVVVVLACV
                     CFCYPEILFHQVIMGPSRGDFFTYTFLCHLGEKWGMGDALCFTQFDFKFQSTDVTGQF
                     TLQMWAALVAGIVIAAPYVLWELWRFIKPALKQKEVRAARGFIFYATGLFLTGVVFSY
                     YVVTPMAVYFLGNYKVDEAIVNNFTIDSFISVVTTLVLLMGLVFELPIVIYFLARFGI
                     ITAKFMRKHRKHAIVVILIVAAVITPTTDVFTQTLVAVPLYILYEASVFVAKRVEKKL
                     AENE"
     gene            5141..5947
                     /locus_tag="VFU15_02160"
     CDS             5141..5947
                     /locus_tag="VFU15_02160"
                     /inference="COORDINATES: protein motif:HMM:NF018917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EI24 domain-containing protein"
                     /protein_id="HEU4716601.1"
                     /translation="MALLDDFSTGISSHFRAWRFIRRHRLWLFFLYPVLLLAVLFPAG
                     IFSIWSLGTSVAGSTWNLVSDNKEHLDTGIGWLNSILLGLLHVITFLAGWVVRILLTL
                     VVWKIARYIVLIFCSPMMARLSERVDEIHCGKKFPFDFRQFLKDTMRGVLVNIRNMLL
                     ESLWVLLLFIIGWIPVIGWLSAFFVIIVGWYFLGFNMMDYSCERRRMRISAGARFIRK
                     HKGIAIGNGMTFSLLLLIPVAGIVIAPVLSPVAATLASLDRLDAHENSKT"
     gene            5989..6738
                     /locus_tag="VFU15_02165"
     CDS             5989..6738
                     /locus_tag="VFU15_02165"
                     /inference="COORDINATES: protein motif:HMM:NF014658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis protein"
                     /protein_id="HEU4716602.1"
                     /translation="MIRISAVSYLNTKPFLYGLNHSGMKDISVSLDTPAECARKLLSG
                     EVETGLVPVAIIPLLKSPWIIPGYGICADGPVESVMLYSNVPLQEIKTVYLDYQSRTS
                     VMLARVLAREHWKISPRWENAEAGFEEKIPADAAAVVIGDRTFRLNGKHAYEFDLAGE
                     WKKFTGLPFVFACWVSNVQPGEKFVSDFRAALEYGLNNTGKVIEEVREKYRPFDVEKY
                     LTSSLKFRMGEKEESGMRLFLSKMKELQPVG"
     gene            6784..8421
                     /locus_tag="VFU15_02170"
     CDS             6784..8421
                     /locus_tag="VFU15_02170"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU4716603.1"
                     /translation="MKQAIQLFGILLFFLVAGNAPAQSNIVDKGGDEVKIFNAQQAFN
                     MGDYVKAVNLYKEVLANKPNDAGIIFHIGECFFSMNEVDKAQEQFEKAKSISSTASPD
                     LDLDLGRCYQMKGHLDSAIAEFSIAKTMNAANAMKLKEIDYYLSQCQTAKSLMATPKP
                     VTVTNLGANVNSPYDDKRPSITSDGKTLIFTSRRPDGKSANRDDEGDGGYFEDIYECS
                     WDSAKMTWGVADLARGAVNTEGHDAACSISPDGRQMFLYVNNAEAGGAGDIYVSKRSS
                     SGKWGTPKNMGKGINTSYFEDGAVLSPDGNTLYFMSERGQDLPWKGQKGYGHSDIWMC
                     KKVDKNSWGDPVNLGPTINTEYDEGGIFPAPDGKTLYFCSNGHNSMGGYDIFMTRFEN
                     GAWTEPVNMGYPINTIANERMFLLTADGKSAYVDSDRSGGMGDRDLWIVDMTELLPKP
                     HTGPYMSLLTGTTYDGDGRPTSVTIDFYDATGATKITSVTSSAAGNYQVSLEGDKTYE
                     IHIDAPGFKPVKESVKLDADKSGATFELVKHFILYKQ"
BASE COUNT         1988 a         2263 c         2240 g         1941 t
ORIGIN      
        1 acaacctgca tgaagcggaa tgttatgcga aaagccaggg caaaccgctc ttcatcgatt
       61 tcaccggtta cgcctgcgtg aattgccgga aaatggagga caacatctgg ccggattcac
      121 gcgtgtatca attcctgagc gaaaagtacg tggttgtttc tctttacgtc gacgacaaga
      181 aagagcttcc cgctgagcag caggtgacgg atgcacaggg aagaagagtg aaaacctacg
      241 ggaacgtctg gagcaacatc gaagcggatt attaccagca gaattcacag cccctgtacg
      301 ttctcatcga tgatgacgga aagctgctcg ctcccccgcg tggctacacg cctgacgcgg
      361 cgaaatatgc ggattatctc cagaaaggtc ttgacgtttt tgaaaagagg aaagccggca
      421 cgaaataatt ttcagtgcct ccgcagctcg aagtttttcc cgagataaac acggcgcacc
      481 tgctcatcgg ctgcaagctc ttccgccgtt cccgctttta agatcgttcc ttcaaacagg
      541 agatacgcgc gatcggtgat ggaaagcgtt tcgtgcacgt tatgatcggt gatgagaatg
      601 ccgatgtttt ttttcttcag cgaagcgaca acaccttgga tgtcttccac ggcgatcggg
      661 tcgacgccgg caaaaggctc atcgagcagg atgaagccgg ggctcgtagc gagacaacgc
      721 gcaatttccg tgcgccgtct ttcaccgccg gacaacgcat tccccatgtt cttccgaatg
      781 tgctgcagcc cgaattcatc cagcaggctt tcgagtttgt tcacctgttc accttttgtc
      841 agcttcgtca tttcaaggac ggcgcggata ttatcttcca ccgtcaggtt gcggaacacg
      901 gaagcttcct gcgcaagata accgatgccg agctgcgcgc gtttgtacat cggctcgcgc
      961 gtgatgtcgc ggtcgtcgag atagatccgg cctgagttcg gcttgatcat tcccacgatc
     1021 atgtaaaagg aagttgtctt cccggcgccg ttgggtccga gcagccccac gatctcaccc
     1081 cgtttcacat tgaccgaaac gcccttgacc acctgccggt tcttgtacga cttgacgata
     1141 ttttccgcac cgaggatcat ctgactgcaa agttgatcaa attacattcc ggaaaagacg
     1201 aaaaaagaaa atgagctgtt aatttttcca ttgtgccgga atcagaagat gatctgcagc
     1261 ccgatgcgca caccgacggg cgccacgttc gggtgatcga gataattgag gcgccagaaa
     1321 caatcgacgc ggaagaaccg gaaaatattt tcgatgccgg cgccgagttc catgtaaggt
     1381 ccgtgatcga gcgaatagag cgtggaagga aaaaggatct cgttcctgtt cgctttgctt
     1441 acgcttccgt aaagcgcgcg gaaggaaagg acttcccgcc atttcagctt gcggatgagc
     1501 ggaattttat tgaagaagaa tccgtcgaaa tggtgaatga gaatagccga tgcgtactgg
     1561 tcgctggcga actcgaaata gttcatcatg ttgtacgccg tgccgtcata aacgtacgtt
     1621 tcatttcccg ggtgcaggat catcagcggg aacggaacgc ggccccaggt cttgccgacc
     1681 tcgaacgtgt aattgagata accgatcggg ttgatgcgga tgcggtcgtc gcagttgatc
     1741 gtcaggcgct ggtacgcgta ctggctgccg aaaacacctt tgaaaccgga cacgtactgc
     1801 acctggaaaa cgggccacag cgttcccacg ctcgtgcgtg aaaaaacacc ttcgacgtat
     1861 ttttcattga acgcaaaacg gatgttcagc cggagctcgg atgtggaaat gcgcggcagc
     1921 gtgtcgaaac ctccgtcgga aagcgggtga atgtaaccgt cgccgagcgg gtacatgttc
     1981 ctgttcacga gcgaaagatg catgttcagc cctttgaaaa tatcgcgctc gtaccaccat
     2041 tcgtattgtt caacccgtgt gtagttggtg aacggcgtgc ggcggaaaat agtcgccatg
     2101 atgttgtcgt tcgagaacgc gttgttgctc agcccgagga tctcgaaatc gtttttgtaa
     2161 ttcacgccgg tgagctgccg cggtttcttg gtgatgaacg ttttgaaacc ggcgccgtat
     2221 ttgaattctt catccttgca cccgtacgcc acgtagccgt tcagctccac ccatttcgaa
     2281 aaatcattcg acgttcgtcc gcccacgcgc agccgcgttc cctcgatgcg gttgtagctg
     2341 acggttttgt accagggacc gtattcaaaa tcgccgagtg ttttatagcc tgtgtatgca
     2401 gtaatcgcta cggtttccca ggttttgtaa atgggaaggt gctgcacgga atcaacgcgc
     2461 gcgtagatct gcctttccgt ccgcgtgagc gaatcgtgtc ttgcattcgc ccagaaggcg
     2521 ctgtcgcggc tttccgcgcc gggctgcacg atgaggttgt ccgtccgcgt ataaaaatca
     2581 tcatccttgc attgattcac gagaatgtcg cggtacgacg tcgtcttcct cccgtaaaaa
     2641 cccatctgct tgttcgtcat gttgaaatcg ccgatcactc tttctttcgt catcatccat
     2701 gccccgctcg cattgtcgta ttcctggatg acgtagaacg aattcacgta attgatgttc
     2761 gcatcatcgg cgatgttcat ttcaatgcgc ttcaccgcga aagccgtgtc ggcgatccac
     2821 atgttcccgt aaaaaagcag ctcctgcttg cgcttcggct tgaactggat ctggtagcac
     2881 cagtgcccgt cgatcaccgt gctgtcgatg aggtaaaaat gatagaacag cgttccgtta
     2941 ttggagatcg ggctgacgaa taatttcccg aagacgagaa tgttgttctt gtaaatgtcg
     3001 acatcctggt acatgtcgcc ggtgaactgc gacacgctgg catcctgtat gcccgaaacc
     3061 tgcgatcctt tgatcacttc cttgtggcat tcggggcttt tcttgtagta aaaatcggag
     3121 atggattcgc tgaagaagat cgggaggttc ggtttttcgc tgggattcgt gctgtcgatg
     3181 tcgtcgaaga tgaacttgac cggcttcatc accttgctct gctggaattt cggtgtgatg
     3241 ttgttgaggt cgaactcgag cttgttgtaa acttcatact ggtagcaatc cagcctgcgc
     3301 ttgtcgttct ggtccttgtg tgcgtaaact tttttcagca gcgtgacagc aggattttcg
     3361 ccgggatgaa tgtcgacgat gtccatctct cccgccactt tcatcgggat gttgagcacc
     3421 tgcggggaaa cgcctttcct gatcgcgacg gtggaaggga ggtaacccac gtaaaccgcg
     3481 cggagggaat cggcgccggc cggggcggtg agtgaaaaat ttccgtcgat atcggaaacc
     3541 gttcctgtgg tcgttcccac aacgatgatg cttacgaagg gcatcgtctc atttgttgcg
     3601 acgttgtata ctttcccggt gatctttacc tgggcaaacg cggaaacaaa caagaaaaag
     3661 gaaaccagta cggacaggat ttttcgcatt cctctttcac cccgttgggt tgtcacgaat
     3721 ataacaaatg atttccgtcc cgttacgtag cgatcgttca ttgaagtccg gaacaaagac
     3781 gaaaaaaacc gcgaatggtt taccggcccg cggtaagttc ttcccaatat tcgacggccc
     3841 gccgggtatg tgggatgacg atcgttcccc cgacgatatt cgccacggca aatacttcga
     3901 agagctcttc tttcgtgacg cctgcttcgt ggcatttccc gaggtggtac ttcacgcagt
     3961 cgtcgcagcg cagcaccatc gaggcgacga ggcccagcat ttccttcgtc ttcgtggaaa
     4021 gcgcgccgtc ctggtaagta ttcgtgtcga ggttgaaaag ccgcttcagc acgagattat
     4081 ccgcagcgag gatcttcccg ttcatttttt cgcggtaggt gttaaactca ctgactgatg
     4141 acatgaccgt ttttgttcaa ggtttagaat tcaaagttca gcgttacgcc cgcagaaaaa
     4201 caggccgcga gcgcgatcat tcgttttcag cgagtttttt ctccacacgt tttgcgacga
     4261 acacgctggc ctcgtaaaga atatacagcg gaacggcgac gagcgtctgc gtgaaaacgt
     4321 ccgttgtcgg cgtgatgacc gcggcaacaa tgaggatgac cacgatcgcg tgcttgcggt
     4381 gcttgcgcat gaatttagcc gtaatgatcc cgaaacgcgc gagaaaataa ataacgatcg
     4441 gcagctcgaa cacgaggccc atgagcagca cgagcgtcgt caccacggaa atgaaggaat
     4501 cgatggtgaa attattgacg atcgcttcat cgaccttgta gttgcccaga aagtaaaccg
     4561 ccatcggcgt cacgacataa tagctgaaca cgacgccggt aagaaaaagc ccggtggcgt
     4621 aaaaaataaa tccgcgcgcg gcgcgcactt ccttctgctt cagcgcaggc ttgatgaaac
     4681 gccagagctc ccataaaaca tacggggcgg cgatcacgat cccggcgacc agcgcagccc
     4741 acatctgcag cgtgaactgc ccggttacgt ctgtgctctg gaatttaaaa tcgaactgcg
     4801 tgaagcaaag cgcatcgccc atgccccatt tttcaccgag gtgacagaga aaagtgtacg
     4861 tgaaaaaatc tccccggctg ggccccatga tgacctggtg gaagaggatc tcgggatagc
     4921 agaaacacac acaggccagc accaccacga cgatcgccga tcgcacaaga tgccagcgca
     4981 gggcctccac gtgtccgagg aaggacatct cgcccgcttt gttcttgttg gtggaaaaaa
     5041 gtcccatgaa agcgcgcaaa gataactcaa agttgcctgt gaagatcaaa cttcacgact
     5101 tgccgcaccc cggcgaaaac cctttccgct acatttgcac atggcattgc tggatgattt
     5161 ctccaccggt atttcctcgc acttccgagc atggcgcttt atccgccggc acaggctgtg
     5221 gctgttcttc ctctaccctg tcctccttct cgccgtcctt tttcccgcag gcattttctc
     5281 gatctggtcg ctgggaacgt ccgtggccgg cagtacgtgg aacctggtca gcgacaacaa
     5341 agaacatctc gacaccggca tcggctggct caacagcatc ctgctcggac tcctccacgt
     5401 gatcacgttc cttgcgggct gggtcgtgcg catcctgctc acgctggtgg tgtggaagat
     5461 cgcgcgctac atcgtgctca ttttctgctc cccgatgatg gcgcggcttt cggagcgcgt
     5521 ggatgagatc cattgcggga aaaaattccc gttcgatttc cgccagttcc tgaaagatac
     5581 gatgcgcggt gtgctggtga acatccgcaa catgctgctc gaatcgcttt gggtgctgct
     5641 gctgttcatc atcgggtgga tcccggtgat cggctggctc tccgcttttt tcgtcatcat
     5701 cgtcggctgg tattttctcg gcttcaacat gatggattat tcctgcgagc ggaggcggat
     5761 gcggatttca gcaggcgcgc gtttcatccg caaacacaaa ggcatcgcca tcggcaacgg
     5821 gatgaccttt tcgctgctgc tgctcatccc cgtcgcgggc atcgtcattg cgcccgtgct
     5881 ttctcccgta gccgccacgc tcgcgtcact cgaccgtctc gacgcacacg aaaattcaaa
     5941 aacctgaact cagcatttta ttttttttat tctgaacacg aaactttcat gatccgcatc
     6001 tccgctgttt cctacctcaa caccaaacct tttctctacg gactgaacca ttcgggaatg
     6061 aaggacattt ccgtttcgct cgacacgccg gcggaatgtg cccgcaagct gctttcgggc
     6121 gaagtggaaa cgggacttgt cccggtggcg atcatcccgc tgctgaaaag cccgtggatc
     6181 atcccgggat acggcatctg cgccgacggt cccgtggaaa gcgtgatgct gtattccaac
     6241 gtaccgctgc aggaaataaa aaccgtttac ctcgattacc agtcgcgcac ctccgtgatg
     6301 ctggcgcgcg tgctggcgcg cgagcactgg aagatttcac cccggtggga aaatgcggaa
     6361 gcgggcttcg aggaaaagat cccggcggat gcggcagcgg ttgtcatcgg cgacaggact
     6421 ttccggctga acgggaaaca tgcgtatgaa ttcgatcttg caggagaatg gaaaaagttc
     6481 accgggcttc cgttcgtgtt cgcctgctgg gtgagcaatg tgcagccggg agaaaagttt
     6541 gtcagcgatt ttcgcgcagc cctggaatat ggattgaaca acaccggcaa ggttattgaa
     6601 gaagtacgtg aaaaataccg gcctttcgat gtggaaaaat acctcacttc atcccttaaa
     6661 ttcaggatgg gagaaaaaga agaaagcggc atgcgacttt tcctctcgaa aatgaaagaa
     6721 ttgcagcccg ttgggtaaga ttctttcggt tgtttatatt tgcttttcta tcccaaaccc
     6781 gttatgaaac aagccataca gctattcgga atcctccttt ttttcctcgt cgcaggaaac
     6841 gctcctgcgc agagcaatat tgtcgataaa ggcggagacg aagtcaagat cttcaacgcg
     6901 cagcaggcgt tcaacatggg tgattatgtg aaagcggtca atctttacaa agaagttctt
     6961 gccaacaagc cgaatgatgc gggtatcatt ttccacatcg gggaatgctt cttcagcatg
     7021 aacgaagtgg acaaagccca ggaacaattt gaaaaagcaa aaagcatcag ctccaccgca
     7081 tcgcctgatc tcgacctcga tctcggtcgc tgttaccaga tgaaaggaca tctcgattcc
     7141 gcgatcgctg aattttccat agccaaaacg atgaacgccg caaatgcaat gaagctgaaa
     7201 gagatcgatt attacctcag ccagtgccag acggcgaaat cactgatggc aacacccaag
     7261 ccggtgacgg tgaccaatct cggcgccaac gtgaacagcc cgtacgacga taagcgtccc
     7321 tccatcacct ctgacggaaa aacgctcatt ttcacttctc gccgtcccga cgggaaaagc
     7381 gccaaccggg atgacgaagg cgacggcggc tatttcgaag acatttacga atgcagctgg
     7441 gacagcgcca aaatgacctg gggcgttgca gatctcgccc gcggcgccgt gaacacggaa
     7501 ggacacgatg ccgcctgttc catctcgccc gacggtcgcc agatgttcct ttacgtcaac
     7561 aacgcggaag cgggcggcgc tggagatatt tacgtttcca agcgctcttc ttcgggcaaa
     7621 tggggaactc cgaagaacat gggaaaaggg atcaacacca gctatttcga ggatggcgct
     7681 gttctttctc ccgacggaaa tacgctttat ttcatgagcg aacgcgggca ggatcttccc
     7741 tggaaaggac agaaaggcta cggccacagc gacatctgga tgtgcaagaa agtggacaag
     7801 aacagctggg gcgatcccgt caacctcggt ccgacgatca acaccgaata cgacgaaggc
     7861 ggaattttcc ccgcgcccga cggaaaaaca ctttacttct gttccaacgg ccacaattcc
     7921 atgggcggat acgacatttt catgacgcgt ttcgagaacg gcgcatggac cgaacccgtc
     7981 aacatgggct acccgatcaa cacgatcgcg aacgaacgca tgttcctgct cacggcggac
     8041 gggaaatccg cttacgtcga ttccgaccgc agcgggggaa tgggcgaccg cgatctgtgg
     8101 atcgtcgaca tgacggagct gctgccgaaa ccgcacacgg gtccttacat gtcgctgctc
     8161 acgggcacga cgtacgacgg ggacggaaga ccgacttccg tcacgatcga cttttatgat
     8221 gcaaccggcg caacgaagat cacatcggtt acttcgagcg cagccggcaa ttaccaggtt
     8281 tccctcgaag gcgacaagac ctacgaaatt cacatcgacg cgccgggctt caagccggtg
     8341 aaagaatcgg taaagctcga tgccgacaaa tcgggcgcga cgttcgagct ggtaaagcat
     8401 ttcatactgt acaagcagta gagagaggca ag
//
LOCUS       DASTSF010000074         3253 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1292747,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000074 DASTSF010000000
VERSION     DASTSF010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3253
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1292747"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /locus_tag="VFU15_02175"
     CDS             <1..346
                     /locus_tag="VFU15_02175"
                     /inference="COORDINATES: protein motif:HMM:NF016270.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HEU4716604.1"
                     /translation="EEAGRVIERIPVIRHIYSPLKDFTSAFIGNKKRFTHPVLVTTNA
                     ASNIREIGFVTGEDLSELGIAKEFVAVYIPMSYAISGRLLVVPKENITALDIPASEAM
                     KFIVSGGVSEVD"
     gene            394..1062
                     /locus_tag="VFU15_02180"
     CDS             394..1062
                     /locus_tag="VFU15_02180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716605.1"
                     /translation="MKKLILLLVTPLMSFHLFADKLKITVDEKSENIGGGNHNALVVT
                     IYDASPDDIIKEWKSKMKDYNAKVSGKDEIFADNALIKEISDNTCDVYAKTEKVSDTE
                     TKFIVGFLLGETWLSSSNNPGPYKAAEKIVKDFATKMTKDAIGDKLKAEQKKLDKLND
                     EQSDLEKKNKDLHDDIDTYNDKIKKAQDDIKTNEDAQTSKKAEIDAQQKAVDDLKARQ
                     DSVE"
     gene            1148..2143
                     /locus_tag="VFU15_02185"
     CDS             1148..2143
                     /locus_tag="VFU15_02185"
                     /inference="COORDINATES: protein motif:HMM:NF006426.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoglucose/phosphomannose
                     isomerase"
                     /protein_id="HEU4716606.1"
                     /translation="MATDMKMLVENFPGQLEEALAIGKKAKLSGASSEIKNVIISGLG
                     GSGIGGTIISELVFDTCPVPVTVTKGYFLPAHADKNSLVIISSYSGNTEETLNCLREA
                     VKKGSHVVCITSGGEVERLAKENRLDIILIPGGMPPRSCLGYSLTQLCFVLVHHRLAP
                     AQLTGDIASSAKLLRERKEEIIAEAKELAGFLNGRTPVIYSTINNEGIAVRFRQQLNE
                     NAKVLCWHHVIPEMNHNELVGWAGGSEEYAVVLFRHHDDYERNRTRIEINKDIISKFT
                     PHIREVYSEGKSAMEKMFYLVHLGDWASVFLAALRDVDAVEVNVINFLKGELAKK"
     gene            2203..2931
                     /gene="lipB"
                     /locus_tag="VFU15_02190"
     CDS             2203..2931
                     /gene="lipB"
                     /locus_tag="VFU15_02190"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:NF010925.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HEU4716607.1"
                     /translation="MNSVCFHDIGLIDYKEGWDFQEKLFHGITARKIANRDLPADEQA
                     PTQSHLIFCEHPHVFTLGKSGDEHHLLANEKQLSEINATFYKINRGGDITYHGPGQLV
                     GYPILDLDNFFTDIHRYLRTLEEIIIRSIAEFGLKGERYPGYTGVWLDPNDPVRARKI
                     CAMGVRSSRWVTMHGWALNVNSDLSYFGNIVACGIPDKGITSLEKELGKKADMTEVKR
                     IVRRHFEEQFDCVLVTEDSQVAER"
     gene            complement(2971..>3253)
                     /locus_tag="VFU15_02195"
     CDS             complement(2971..>3253)
                     /locus_tag="VFU15_02195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716608.1"
                     /translation="YSGRMGKHFLSQNAFFVYAGTYLTLVLQLAFPLTIWFRKTRTVT
                     MITGIVIHLLIIFLMGITDFGLIMIAAYSLFVKDGAAGKPAQSENPRAV"
BASE COUNT          868 a          849 c          867 g          669 t
ORIGIN      
        1 cgaagaagcg ggaagggtca ttgaacgcat cccggtgatc cggcacatct attccccgct
       61 gaaggatttt acctcggcat tcatcggcaa caaaaaaaga tttacgcacc cggtgcttgt
      121 gaccacgaac gccgcaagca acatccgcga gatcggtttt gttaccggcg aagatctttc
      181 ggagctgggg atcgccaaag aattcgtcgc cgtttatatt cccatgtcgt acgcgatctc
      241 ggggcgtctc ctcgtcgttc cgaaggaaaa tatcaccgcg ctggacatac cggcttcgga
      301 agccatgaag tttatcgttt cgggcggcgt ctccgaagtt gactgaattt ggcgtaactt
      361 ttgaatatta tttcaacaaa caacacagcc cacatgaaaa agctgatact actcctcgta
      421 acaccgctga tgtccttcca tctttttgcc gataaactca agatcacggt ggatgaaaaa
      481 agtgaaaata tcggaggcgg aaaccataac gcgctggtcg ttaccattta cgatgcttca
      541 ccggatgaca tcataaagga atggaaatcg aaaatgaagg attacaacgc caaggtgagc
      601 gggaaagacg agatcttcgc cgacaacgcg ctgatcaagg agatcagcga caacacgtgc
      661 gatgtgtatg ccaaaacgga aaaggtcagc gataccgaaa cgaaattcat cgtcgggttc
      721 ctcctcgggg aaacctggct cagctcctcc aacaatcccg gtccgtacaa agccgctgaa
      781 aagatcgtga aggatttcgc cacgaaaatg accaaggacg cgatcggcga caagctgaaa
      841 gcggagcaga aaaagctcga caagctgaac gatgagcaat ccgatctcga gaaaaagaac
      901 aaagacctcc acgacgatat cgacacctac aacgacaaga tcaagaaagc gcaggacgac
      961 atcaagacga acgaggatgc gcagacgtcc aaaaaagcgg agatcgatgc acagcagaaa
     1021 gcagtggatg acctgaaagc acggcaggat tcggtggagt gaggcgcgtg tatttccatc
     1081 cgttacgctt tgttaacgcg gaaaggcgac ctgacctttt cgtaactttg tgattcatct
     1141 gaagaacatg gcaacagaca tgaaaatgct cgtggagaat tttcccgggc agcttgaaga
     1201 agcgctggcg atcggtaaga aagcgaagct ttcgggtgca tcttctgaaa taaagaatgt
     1261 gatcatttcc gggctcggcg gctccggcat cggcggcacc atcatctccg agctggtttt
     1321 cgatacgtgc cccgttccgg ttacggttac aaaaggctat tttctcccgg cccatgcgga
     1381 taaaaattcg ctcgtgatca tttcgtcgta ttcgggcaat acggaagaaa cgctcaactg
     1441 cctgcgtgaa gcggtgaaga aaggctcgca cgtggtgtgc atcacgagcg gcggagaagt
     1501 ggaacgtctc gcgaaggaga acaggctcga tattattctc atcccgggcg gaatgccgcc
     1561 gcgctcctgc ctcggctatt cgctcacgca gctctgtttt gttttggtgc atcacaggct
     1621 cgcgcccgca cagctgaccg gtgatattgc ttcttcggca aagcttttac gcgagcggaa
     1681 agaggagatc attgcggaag caaaagagct ggccggtttt ctcaacggcc gcacgccggt
     1741 tatttattcc acgatcaaca acgaaggaat tgcagtgcgt ttccgccagc agctgaatga
     1801 aaatgcgaag gtgctctgct ggcatcacgt catcccggaa atgaaccaca acgagctggt
     1861 gggctgggcc ggcggatcgg aagaatatgc cgtcgtgctt ttccgccacc acgacgatta
     1921 cgaacgcaac cgtacgcgca tcgaaataaa caaggacatc atttccaaat ttacgccgca
     1981 catccgcgaa gtgtattccg aaggaaaatc ggccatggag aaaatgttct acctcgtgca
     2041 tctcggcgac tgggcatcgg tgttcctcgc tgcgctgcgt gatgtggatg cggtggaagt
     2101 gaacgtgatc aacttcctga aaggcgagct ggcgaaaaaa tgaggaatca tcagtggtga
     2161 atgataaatg atgaatgata agtaacgggt tttatcattt ccatgaacag cgtttgtttc
     2221 cacgatatcg gtctcatcga ctacaaggaa ggctgggact tccaggaaaa gttattccac
     2281 gggatcactg cgcgcaaaat cgcgaaccgc gatcttcccg cggatgagca ggcgcccacg
     2341 caaagccatc tcattttctg cgaacacccg cacgtgttca cgctcggcaa aagcggcgac
     2401 gagcatcacc tgctggcgaa tgaaaaacag ctcagcgaga tcaacgcgac gttctataaa
     2461 ataaaccgcg gcggcgacat cacgtaccac ggacccgggc agctcgtggg ttacccgatt
     2521 ctcgacctcg ataatttttt caccgacatt caccgctacc tccgcacgct ggaggaaatc
     2581 atcatccgct ccattgcgga attcggactg aaaggcgaac gctatcccgg ctacacgggt
     2641 gtgtggctcg atccgaacga ccccgtgcgc gcccgcaaga tctgcgcgat gggcgtacgc
     2701 agcagccgct gggttaccat gcacggctgg gcgctcaacg tcaattccga tctttcctat
     2761 ttcgggaaca tcgtcgcctg cgggatcccc gacaaaggca tcacttcact cgaaaaagag
     2821 ctggggaaaa aagccgacat gaccgaagtg aagcgcatcg tacgcaggca tttcgaagag
     2881 caattcgact gtgtgctcgt gacggaagat tcacaggttg ctgaacgata acgaagcact
     2941 tctttcgcgg tttgcggttt cagcgacgga ctaaacggcc cggggatttt cggattgcgc
     3001 cggctttccg gctgcaccgt ccttcacgaa aagcgaatac gccgcgatca tgatcagccc
     3061 gaaatccgtg atgcccatca ggaaaatgat cagcaggtga atgacgatcc ccgtgatcat
     3121 tgtgaccgtt ctcgttttcc ggaaccagat cgtgagcggg aaagcgagct gcagcacgag
     3181 cgtgaggtac gtccccgcgt aaacgaaaaa tgcattctgc gaaagaaaat gcttccccat
     3241 ccggccggaa tat
//
LOCUS       DASTSF010000075         4389 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1976515,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000075 DASTSF010000000
VERSION     DASTSF010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4389
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1976515"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /locus_tag="VFU15_02200"
     CDS             <1..295
                     /locus_tag="VFU15_02200"
                     /inference="COORDINATES: protein motif:HMM:NF017027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716609.1"
                     /translation="LMADAYDVNLYFVPMFTGVKQSAAGNAKKQMKQDVQEDIQPHII
                     VYKGELDAYKETLKMDDKKKPYVFVLDKDGKIVFVTSGPYTEDKMDQIDDNIE"
     gene            complement(343..504)
                     /locus_tag="VFU15_02205"
     CDS             complement(343..504)
                     /locus_tag="VFU15_02205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716610.1"
                     /translation="MKKGRSRKPALKQKPITGDVIKLQVDYRTTIVVRSQRALEMWME
                     KYPNAKIVA"
     gene            complement(866..1015)
                     /locus_tag="VFU15_02210"
     CDS             complement(866..1015)
                     /locus_tag="VFU15_02210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716611.1"
                     /translation="MKRQKSLKPAVKAGDRIKLRIDYRTVIVVRSQEALAMWMSKFPA
                     AQIID"
     gene            complement(1337..3970)
                     /gene="clpB"
                     /locus_tag="VFU15_02215"
     CDS             complement(1337..3970)
                     /gene="clpB"
                     /locus_tag="VFU15_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017259073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HEU4716612.1"
                     /translation="MNLNNFTLKSQEAIQQAQQLAMTQSNQAVETGHLLKGILAVDEN
                     VTPFLLKKLNVNPALISQVVDRIVESYPKVSGGGQYLSNNANQAIVKAVNSLKDFNDE
                     FVSVEHLLIGLLETGDQVSQLLKDNGISIKDLKSAIKELRKGDRVTSASQEETYNALS
                     KYAVNLNERARSGKLDPVIGRDEEIRRVLQILSRRTKNNPILVGEPGVGKTAIAEGLA
                     HRIINGDVPENLKSKIIYALDMGALIAGAKYKGEFEERLKAVVKEVISSEGDIILFID
                     EIHTLVGAGGGEGAMDAANILKPALARGELRAIGATTLNEFQKYFEKDKALERRFQKV
                     LVDEPSAEDAISILRGIKEKYETHHKVRIKDEAIISAVELSQRYISDRFLPDKAIDLI
                     DEAASKLRMEINSMPAELEDVERKIRQLEIEREAIKRENDKEKLEHLNREIAELNDKR
                     ADLKAQWQSEKEVVENIQKEKEKIEQLKFEAEQAERAGDYGKVAEIRYGKIKEAEDKL
                     NSAQEQLAKMQSSGNPMIKEEVDSEDIAGVVSLWTGIPVTRMLQSEREKLLHLEGELH
                     KRVIGQDEAISAISDAVRRSRAGLQDSKKPIGSFIFLGTTGVGKTELAKALAEFLFNN
                     ENAMTRIDMSEYQERHSVSRLVGAPPGYVGYEEGGQLTEAVRRKPYSVVLLDEIEKAH
                     PDVFNILLQVLDDGRLTDNKGRVVNFKNTIIIMTSNLGSHIIQENFEKLDHGDREEIL
                     AKTKAEVFELLKKTIRPEFLNRIDEIIMFSPLSREDVEEIVKLQMAQLEKMLEENHIT
                     LKASPEAIAWLSELGYDPQFGARPVKRLIQKKVLNELSKQILAGKVHKNEEVVLDVFD
                     KEIVFRQPVESDSGAKKSRKK"
     gene            complement(4196..>4389)
                     /locus_tag="VFU15_02220"
     CDS             complement(4196..>4389)
                     /locus_tag="VFU15_02220"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEU4716613.1"
                     /translation="LNNAQNITINMVNTLGQVVSTETRELSAGNNLIQMNTADLPNGI
                     YFLNVISGDSQLTSKFVKQ"
BASE COUNT          947 a         1104 c         1058 g         1280 t
ORIGIN      
        1 gctcatggcg gatgcgtacg acgtgaactt atattttgtg ccgatgttca ccggtgtaaa
       61 gcagagcgct gccggaaatg ccaaaaaaca gatgaagcag gacgtccagg aggatattca
      121 gccgcatatt attgtttaca aaggcgagct ggacgcgtac aaggaaacgc ttaaaatgga
      181 cgacaagaag aaaccgtatg tgttcgtcct cgataaagac ggaaagatcg ttttcgttac
      241 ttcaggtccg tacacggagg ataaaatgga ccagatcgat gataatatcg aataaaaaat
      301 aaaaaacccg gaagccgttt cagaattgtg aaacggcttt tttcaggcaa cgatttttgc
      361 gttcgggtac ttttccatcc acatttcgag cgcacgctgc gaacgtacca cgatcgtcgt
      421 gcggtaatcc acctgcagct tgataacgtc gcccgttatg ggtttttgct tgagggccgg
      481 tttgcggctc ctgccttttt tcatttgtgc actttcacgt gctaaaacca aaggtctcat
      541 gattgcttgt tttaaaatta ttactctaca aaagaaagcc tgaaatcaaa aggaaggccc
      601 gtcttttctt cggttgggaa cagccacccg atgagaggtc gactttggca gttaagcggg
      661 gcaggggcga tcctgttcat taattgtgaa attccttttg aaaagttgtt tgtacttgtt
      721 ttatttcctg ttaatatgct ggtaattatt gttttgtgtg actttattga aatgtgaaga
      781 tcagcttcga aacaaaaagc atcacggtaa ttccatgatg cttttcgggc aaagcccggg
      841 gatgtgttgt ggttgcaacc aaaccttaat cgatgatctg cgcggcgggg aatttcgaca
      901 tccacatggc cagcgcttcc tggctgcgta caacgatcac tgtcctgtaa tcgatcctga
      961 gctttatgcg gtcgcctgct ttgacggccg gtttgagtga tttctgacgt ttcatagctt
     1021 tcgtttttta ttgttagggg gaagattcta tctataagac gtccagtatc gtgccgttgt
     1081 tttaatgctt aacatctttt gacattaaaa caatttataa cgtcatcccg ctgtttggtt
     1141 acgccttttc cgtctttatt tttcattgca tacgaagcgc aatttcgccg cgttcttcag
     1201 tagatattct cccgcccgcc ggtcccggag gccacaggat gaccggtact ttaatctcgt
     1261 actttggtgt aggaaccggt tcctgaacag tggaccggcg gacggggaac tatcgcggcg
     1321 aaattaataa gcgcggttac tttttccggg attttttcgc gccactgtcc gattcaaccg
     1381 gttgccggaa cacgatctcc ttatcgaaaa catccagcac cacttcctcg ttcttgtgca
     1441 cttttccagc caggatctgt ttcgataatt cgttcagcac ttttttctgg atcagccgct
     1501 tgaccggtct tgccccgaac tgcggatcat aaccgagttc ggaaagccac gcgatcgctt
     1561 ccggcgatgc tttcagcgta atgtggtttt cttccagcat tttttccagc tgcgccatct
     1621 gcagcttcac gatctcttcc acgtcttcgc gtgaaagcgg ggagaacatg atgatctcat
     1681 cgatacggtt gagaaattcc ggccggatcg tttttttcag gagctcgaaa acttccgctt
     1741 tcgttttcgc aagtatctct tcacggtcgc catgatccag cttttcaaaa ttttcctgga
     1801 tgatgtgcga tccgagattc gaggtcatga tgatgatcgt attcttgaaa ttcacgacgc
     1861 ggcctttatt atccgtaagc cttccgtcat caagcacctg caggagaata ttgaaaacat
     1921 ccgggtgcgc tttttcgatc tcgtcgagca gaacaacgct gtaaggtttt ctccgtaccg
     1981 cttccgtgag ctgtccgcct tcttcgtagc cgacgtatcc cgggggagcg cccaccagtc
     2041 gcgaaacgct gtgtctttcc tggtattcgc tcatgtcgat gcgggtcatc gcattttcat
     2101 tgttgaacag gaattcagcc agcgcttttg ccagctcggt ttttcccaca ccggtcgttc
     2161 cgagaaaaat gaacgagccg atcggttttt tggaatcctg gagacctgcg cggcttctgc
     2221 gtacggcatc ggaaatggcg gatatcgctt cgtcctggcc gatcacgcgt ttatgcaact
     2281 cgccttcgag gtgaagcagc ttttcgcgct cgctctggag catgcgtgtc accgggattc
     2341 ctgtccacag gcttaccacg cccgcaatat cctcgctgtc gacttcttcc ttgatcatcg
     2401 ggttgccgga agattgcatc ttcgccagtt gttcctgcgc gctgttcagc ttgtcctcgg
     2461 cctctttgat cttgccatag cggatctcgg ccacttttcc gtaatcgccc gcacgttccg
     2521 cctgctccgc ttcgaatttc agttgttcga tcttctcttt ttctttctgg atgttttcga
     2581 ccacttcttt ttccgattgc cactgcgctt tcaggtcggc gcgtttatcg ttgagctcgg
     2641 caatttccct gttaaggtgc tcgagcttct ccttatcgtt ttcacgcttg atggcttcgc
     2701 gttcaatttc aagctggcgg atctttctct caacatcttc cagctcggcc ggcatagaat
     2761 tgatctccat ccgcagcttt gacgctgctt catcgatcag gtcgattgcc ttatcgggga
     2821 gaaaacggtc gctgatgtag cgctgggaaa gctccaccgc cgagatgatc gcttcgtctt
     2881 tgatgcgcac tttgtggtgc gtttcatatt tttccttgat gccgcgcaga atcgaaatgg
     2941 cgtcttcggc agaaggctcg tccaccagca ctttctggaa gcggcgttca agcgctttgt
     3001 ctttttcaaa gtatttctgg aactcgttca gcgtggtggc accgatcgct ctcagctcgc
     3061 cgcgtgccag cgcaggcttg agaatattcg cagcatccat cgcaccttcg ccaccgcctg
     3121 cgcctacaag cgtatggatc tcatctatga aaagaatgat atctccctcc gacgaaatca
     3181 cttctttcac gaccgccttc aggcgttctt cgaattcccc tttgtatttc gctccggcga
     3241 tcagcgcgcc catgtcgagc gcatagataa ttttgctctt caggttttcc ggcacatcgc
     3301 cgttgatgat acggtgggcg agaccttcgg cgatcgccgt tttgcctacg ccgggttcac
     3361 caacgagaat gggattattt ttcgttcgcc tgctgagtat ctgcagcaca cggcggatct
     3421 cttcatcacg cccgatcacg ggatcaagct ttccgcttcg tgcgcgttca ttgaggttga
     3481 cggcatattt ggaaagggca ttgtatgttt cttcctggga agcgctggtt acacgatctc
     3541 ccttacgaag ctctttgatc gcggatttca ggtctttgat cgagatgccg ttatctttca
     3601 gcagttggga aacctggtcg ccggtttcca gcagaccgat cagcaggtgt tccaccgaaa
     3661 caaattcatc attaaaatcc ttaaggctgt tgactgcctt aacgatcgcc tggtttgcat
     3721 tgttggacaa atactgtccg ccacccgaaa ctttcggata cgactccacg atgcgatcca
     3781 ccacctggct gatcagggca gggttcacgt tcagcttttt cagcaggaat ggagttacat
     3841 tttcatcgac ggcaagaatt cctttcagca ggtgaccggt ttcgaccgcc tggttgcttt
     3901 gcgtcatcgc aagctgctgg gcctgttgaa tggcttcctg cgatttgagg gtaaagttgt
     3961 tcaggttcat aatgactaat aataaaatga ttacgaagta aaatctattc tcacgcttta
     4021 ccgatgacca aaatacatac ccattaatga ttttcggaag aagaacgtaa ttctggcagc
     4081 cgcgtctgta atttactgac ggaaaggcgg gtcgtgctga aaaacatgga attatggcgc
     4141 aaaaaaaaac tcccgcccgg aggcaggagt tttaacggga aagaatgtta agcaattact
     4201 gcttgacgaa tttcgaagtc agctggctgt ccccggaaat tacgttcagg aagtaaatac
     4261 cgttaggaag atcagcggta ttcatctgga tcaggttgtt gccggcagaa agctcacgcg
     4321 tttccgtgct cacaacttgt ccaagtgtat tcaccatgtt gatcgtaatg ttttgagcgt
     4381 tgttcagct
//
LOCUS       DASTSF010000076         5019 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1291506,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000076 DASTSF010000000
VERSION     DASTSF010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5019
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1291506"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..284)
                     /locus_tag="VFU15_02225"
     CDS             complement(<1..284)
                     /locus_tag="VFU15_02225"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016991021.1"
                     /note="catalyzes the formation of L-tryptophan from
                     L-serine and 1-(indol-3-yl)glycerol 3-phosphate; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEU4716614.1"
                     /translation="MEFHVTEKGYYGEFGGAFIPELLWPNVEELRLRYRGIMADENFT
                     RELDALLQDYVGRPTPLYHAERLSEKYGTGIFLKREDLCHTGAHKINNTVG"
     gene            complement(265..918)
                     /locus_tag="VFU15_02230"
     CDS             complement(265..918)
                     /locus_tag="VFU15_02230"
                     /inference="COORDINATES: protein motif:HMM:NF012900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEU4716615.1"
                     /translation="MLIKVCGISCDEDLAALSRTGIDFAGFIFVESSPRYIDGKIDPE
                     LCALLSRSVPSLRKTGVFMDETEENIRENAERYHLDAVQLHGNETPEECARFSGELIV
                     LKAFPVNGEESFDETASYEGTCDYFLFDAKGENAGGNGISFDWTLLRNYEGSTPFLLG
                     GGIGLGDAEKIYAIDHPMFAGIDINSRFELYPGKKNIASVTSFVNAFKNTGYGISRY"
     gene            complement(920..1750)
                     /gene="trpC"
                     /locus_tag="VFU15_02235"
     CDS             complement(920..1750)
                     /gene="trpC"
                     /locus_tag="VFU15_02235"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018478043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEU4716616.1"
                     /translation="METILEKIIAYKRKETEERKSLYPVKLLERSLYFQAQPVSLEKY
                     LLREDLSGVIAEFKRRSPSKGTINAYAKPAEVTLAYMQAGASALSVLTDEHFFGGAAK
                     DLEEARRVNYCPVLRKDFIVDEYQVIEAKSIGADAILLIAAVLSKEEIKNLSALAFSL
                     GMEVLLELHDETETARMTGDIRLAGINNRNLADFSVNVERSFEMAEKLGSGFTLVSES
                     GISDPATAANLYDAGFRGFLIGESFMRTAQPGKTCSAFVSQFRELREKNKINNSVISS
                     "
     gene            complement(1765..2757)
                     /gene="trpD"
                     /locus_tag="VFU15_02240"
     CDS             complement(1765..2757)
                     /gene="trpD"
                     /locus_tag="VFU15_02240"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013632463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEU4716617.1"
                     /translation="MKNILEKLFAHETLTQGEAEQVLVSIAEGKSNDAQLASFMTVYL
                     MRSITPDELRGFRDALLQLCSPVDLPDTQAIDLCGTGGDGKNTFNISTLASFVVAGAG
                     GKVIKHGNYGVSSVSGSSNVLEHFGYRFTTDVKMLEHSLERSGVCFLHAPLFHPALKK
                     VAPVRKQLGMKTFFNMLGPLVNPARPRYRVTGVFSPELARVYNYLAQEENENYLIVHA
                     LDGYDEVSLTGTVKIFSRSGERLSDAESFGMKKVQPVEILGGTTVEEAAAVFQDVLSN
                     KGTVAQENVVIANAALALQCLGLHAEISDAVCAARESLHSGKALRSFSQLLSAN"
     gene            complement(2759..3328)
                     /locus_tag="VFU15_02245"
     CDS             complement(2759..3328)
                     /locus_tag="VFU15_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEU4716618.1"
                     /translation="MKVLVFDNYDSFTFNLVHLVEKITGEKPDVFRNDELEIAAAEKY
                     DKIILSPGPGIPAEAGLLLPLIKTYAPTKSMFGVCLGQQAIAEAFGAQLYNLENVFHG
                     VATTIVTEKDPGFLFRNIPQRFQAGRYHSWVVNEKTLPAELVVTARDEEGRIMALQHR
                     LFNVAGVQFHPESIMTEFGEELMKNFLQQ"
     gene            complement(3333..4814)
                     /locus_tag="VFU15_02250"
     CDS             complement(3333..4814)
                     /locus_tag="VFU15_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010853522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HEU4716619.1"
                     /translation="METITEKEKTKKQSAHAGKIPVRIVVRTLPADTCTPVGLYLRLR
                     DRVTEKCTAGCSTVLLESADNAEGRNSRSIIATEPIANVQVKGGKISYEVPQANIYFE
                     ATADREVPEILQQFLGLFEADENCPPGIPAGLIGYTSYDAVRCFEKVQLPAVDDNTVL
                     LRYSLYRTLIVFDHLRDTLRIVELLLPGDESTAGGVETVIFNRDVPRFSFEKKGEEIS
                     PATDERFLEMVEAGKAHCRRGDVFQVVLSRQFTQKFSGDEFNVYRALRMINPSPYLFF
                     ADYGDYRIFGSSPEAQLVVQPSEDKEKFTAVLHPIAGTFRRSGSPKEDRELEEKLLAD
                     PKENAEHVMLVDLARNDLSRSAKNVKVDAFREVHRYSHVIHLVSKVSGETNRGENNFR
                     LLAGTFPAGTLSGAPKIRAMQIIAEQENVPRGFYGGCIGFAGFDGSLNQAIMIRSFIS
                     RNGELVYRAGAGIVAASEPEKELQEINNKLEALRLAMVRAEKM"
BASE COUNT         1114 a         1433 c         1254 g         1218 t
ORIGIN      
        1 cccaccgtgt tgttgatctt gtgcgcgccc gtgtgacaca ggtcttcgcg tttcaggaaa
       61 atacccgttc cgtatttttc cgaaaggcgc tccgcgtgat agagcggcgt cgggcgtccc
      121 acgtaatcct gcagcagcgc atcgagctcc cgggtgaaat tttcatcggc catgatcccg
      181 cggtaacgca ggcgaagctc ttccacgttc ggccacaaaa gttccgggat aaatgctcct
      241 ccgaattctc cgtagtatcc tttttcagta acgtgaaatt ccatagcctg tgtttttgaa
      301 tgcattgaca aacgaggtaa ctgaagcgat atttttttta cccgggtata gctcgaaacg
      361 gctgttgatg tcgatgccgg caaacatggg gtgatcgatc gcgtagattt tttccgcatc
      421 acccagacct attcccccgc ccagcaaaaa aggagtggat ccttcataat tgcgaagcaa
      481 agtccagtcg aaggaaattc cgttgccccc ggcattctct ccttttgcat cgaacaggaa
      541 ataatcacac gtcccttcgt agctggccgt ttcatcaaaa ctttcctcac cgtttacagg
      601 gaacgctttc agcacgatca gctctcccga aaaccgcgca cattcttccg gagtttcatt
      661 cccgtgcagc tgaaccgcat cgagatgata acgttctgcg ttctcccgga tattttcttc
      721 ggtctcatcc atgaacacgc ccgtttttct cagcgaagga acggagcgcg aaagcagcgc
      781 acacagctcc gggtcgatct tcccgtcgat gtaccgcggt gaggattcca cgaagatgaa
      841 tccggcaaaa tcaattcccg tacgcgacag cgcagcgaga tcttcatcac aggaaattcc
      901 gcaaaccttg atcagcattt cacgaagaga ttacgctgtt gtttatttta tttttttcac
      961 gcaattccct gaactgcgaa acgaaagcgg aacatgtctt cccgggttgc gcagtgcgca
     1021 tgaagctttc gccgataaga aatccacgga agcctgcatc atacaggttc gcagccgttg
     1081 cggggtcgga aatcccgctc tcggaaacca gtgtaaatcc gctgcccagc ttttccgcca
     1141 tttcaaacga tcgctctacg ttcaccgaaa agtcagcgag gttccggttg ttgatcccgg
     1201 cgagccggat gtcgccggtc atgcgggcgg tttcagtttc atcgtgcagc tcgagcagta
     1261 cttccatacc gagtgaaaag gcaagcgcgg aaaggttttt tatttcctct tttgacaata
     1321 cggcggcgat cagcaggatg gcgtctgcgc cgatggactt cgcctcgatg acctggtatt
     1381 catccacgat aaaatctttc cgcagcaccg ggcaatagtt cacgcggcgc gcttcctcca
     1441 ggtctttggc agcgcctccg aaaaaatgct catccgtcag caccgacagc gcgctcgcac
     1501 ccgcctgcat atacgccagc gttacctccg caggcttcgc atacgcattg atcgtccctt
     1561 tcgacggcga tctccgcttg aattctgcga tcacgccact gaggtcttcc cgcagcaaat
     1621 atttttccag cgacacgggt tgggcctgga agtaaagact gcgttcgagc aatttcaccg
     1681 ggtaaagcga tttccgctct tccgtttctt tccgtttgta tgcgatgatc ttttcgagga
     1741 tagtttccat gcttttctcc tgttttaatt cgccgaaagc agctggctga atgaacgcaa
     1801 tgcctttccc gagtgaagcg attcccgcgc cgcacacacc gcatcgctga tctccgcatg
     1861 aagaccgaga cattgcagcg ccagcgccgc attggcaatc acgacgttct cctgcgcaac
     1921 ggttcctttg tttgaaagaa catcctggaa aacagcggcc gcttcttcca ccgtcgttcc
     1981 gccgaggatt tccacgggct gaactttttt catgccgaac gattcagcat cggaaagtct
     2041 ttcgccgctg cgcgaaaata ttttgaccgt tcccgtcagc gaaacttcgt cataaccgtc
     2101 gagcgcgtga acgatgagat aattttcatt ttcctcctgc gcgaggtaat tgtatacccg
     2161 cgccagctcg ggactgaaca ctcccgtaac gcgatagcgg ggccgtgcgg gattaacgag
     2221 cggacccagc atgttgaaaa atgttttcat gccgagctgc ttccgcacgg gcgccacttt
     2281 tttcagggct gggtgaaaca acggcgcgtg cagaaaacat acacctgatc tttccagcga
     2341 atgttccaac attttcacat cggtcgtgaa gcggtacccg aaatgctcga gcacattcga
     2401 agaaccactg acggaagaca cgccgtaatt gccgtgcttg atcacctttc cgcctgctcc
     2461 cgccacgaca aaagaagcga gcgtggatat attgaatgtg ttcttcccgt caccgcccgt
     2521 gccgcagaga tctatcgcct gcgtatccgg gagatcgacc gggctgcaca actgcaggag
     2581 cgcatcgcgg aaaccacgca gctcgtccgg cgtaatgctg cgcatcaggt aaaccgtcat
     2641 gaacgacgcc agctgcgcgt cattcgactt tccttccgct atggaaacaa gcacctgttc
     2701 cgcttcgccc tgcgtcagtg tttcatgcgc aaaaagtttt tcgagaatgt ttttcatttt
     2761 actgctgtaa aaaatttttc atcagctctt caccgaactc cgtcatgatg ctttcggggt
     2821 ggaactgcac gccggccaca ttaaaaagac gatgctgcaa cgccatgatc cttccctctt
     2881 catcgcgtgc cgtaactacc agctcagccg gcagcgtttt ttcgttcacc acccaggaat
     2941 ggtaacggcc tgcctggaaa cgctgcggga tattccggaa aagaaaaccg ggatcttttt
     3001 ccgttacgat cgtcgttgcg acgccgtgaa aaacattttc caggttatac agctgcgcac
     3061 cgaacgcttc ggcaatggcc tgctgcccga gacaaacgcc gaacatgctt ttggtcggcg
     3121 cgtacgtttt gatcagcggg aggagaagac cggcttctgc aggaattccc ggacccggcg
     3181 agagaatgat cttatcgtat ttctccgccg ccgcaatttc cagctcgtcg ttacggaaga
     3241 catccggttt ttcgcccgtg atcttttcca cgagatgaac gaggttgaac gtgaacgaat
     3301 cgtaattatc gaaaacaaga actttcatgg cgttacattt tttctgcacg gaccatcgcg
     3361 agccgcaatg cttccagctt gttgtttatt tcctgcaatt ctttttccgg ttccgaagca
     3421 gcaacgatgc cggcgccagc ccggtatacc agctcgccgt tcctgctgat gaacgaacgg
     3481 atcatgatcg cctggttgag cgatccgtcg aatcctgcaa aaccgatgca gccgccgtag
     3541 aatccgcgcg gcacattttc ctgctccgcg atgatctgca tcgcgcggat cttgggcgca
     3601 ccggaaagcg tcccggccgg gaacgttcct gcaagcaggc ggaaattatt ttctccgcgg
     3661 ttagtttccc cgctcacttt cgaaacgaga tgaatgacgt gcgaataacg atgcacttcc
     3721 cggaacgcat ccactttaac gttcttcgcc gaacgggaca aatcgttccg agccaggtcg
     3781 acgagcatga cgtgctctgc attttctttc ggatcggcga gcagtttttc ttccagctca
     3841 cggtcctcct tcggtgatcc gctcctgcgg aacgttcccg caatgggatg aaggacggcg
     3901 gtgaattttt ctttgtcttc cgaaggctgc accacgagct gcgcttccgg cgagcttccg
     3961 aagatgcggt aatcgccgta atccgcgaag aaaagataag gcgaaggatt gatcatgcgc
     4021 agcgcacggt acacattgaa ttcatcgccg gaaaatttct gcgtgaactg gcgcgaaagg
     4081 acgacctgga aaacatcacc gcggcggcag tgcgctttcc ccgcttccac catttccagg
     4141 aaacgttcat ccgtcgccgg tgaaatttct tctccctttt tttcgaatga aaaacgcggc
     4201 acatcgcggt tgaagatcac cgtttccacc ccgccggccg tcgattcatc tcccggcagc
     4261 agcagctcaa cgatccggag cgtatcgcgc aggtgatcga aaacgatgag tgtgcggtac
     4321 agcgaataac gcagcaaaac cgtgttatca tcgaccgcgg gcagctggac tttctcgaag
     4381 caacgcaccg catcgtagct cgtatagccg atgagcccgg cgggaatgcc cggaggacag
     4441 ttttcatccg cttcgaaaag cccgagaaac tgctgaagta tttccggcac ttcgcggtca
     4501 gccgttgctt cgaagtaaat attcgcctgc gggacttcgt acgaaatttt tccacctttc
     4561 acctgcacgt tcgcgatggg ttcggttgcg ataatggaac ggctgttccg gccttcggcg
     4621 ttgtccgcgc tttcgagcag gacggtcgag cacccggccg tgcatttttc cgtcacgcgg
     4681 tcgcgcaggc gcaggtaaag tcccacgggc gtgcacgtat ccgccggcaa tgtgcgcacg
     4741 acgatccgca cgggaatttt cccggcatgc gcggattgtt tttttgtctt ttctttttct
     4801 gtgatcgttt ccatgggaaa tgtgctaaaa aacgaaagcc cgctgtgtca gcgggccttg
     4861 tcgtttatgg ttgttcaaca aaaagcagcg tcacacagct gaaggatttt cacttgcgtg
     4921 ccaccaatgt ttgctgatcg tgatcgtttt catgcggcgc aaagataaag acagaaaaat
     4981 gagatttcca aatccgcgac attaaactcc ggatcccag
//
LOCUS       DASTSF010000077         4054 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_173292,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000077 DASTSF010000000
VERSION     DASTSF010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4054
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173292"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..783
                     /locus_tag="VFU15_02255"
     CDS             <1..783
                     /locus_tag="VFU15_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019669984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative PEP-binding protein"
                     /protein_id="HEU4716620.1"
                     /translation="LSMAVATMACAFYPREVIVRMSDFKTNEYANLIGGKEFEPAEEN
                     PMIGFRGASRYYHDRYREGFRLECQAMKRVRDNMGLTNVKLMIPFCRTVEEGKKVIAE
                     MKKNGLVRGKNGLEIYVMAEIPSNILLAEEFAKIFDGFSIGSNDLTQLTLGIDRDNSV
                     ISPLFNEKDEAAKQLIATLIAKAKKAGVKVGLCGQAPSDFPDYAQFLVESGIDSISFN
                     SDALMNGIANMNKAEELSAKKKNGRSASSSIRNHKKLQNENT"
     gene            770..1528
                     /locus_tag="VFU15_02260"
     CDS             770..1528
                     /locus_tag="VFU15_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007784667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU4716621.1"
                     /translation="MKTLENRTAVITGAASGMGKAMAELFASEGANVIAADRDEKDLA
                     EVVKMIREKKGNVTAVVCDVTKESDVKNMIDKAVETYGGLDILVNNAGVLDDFMPVAE
                     VSDDVWKRVMDVNLNGPFYACRLAVPVMLKKGKGSIINVASVGGLFGARAGVAYTTSK
                     HAVIGLTKNVGYMYGDKGIRCNAIAPGGVNTNIGKGMNPNPLGYAKLSLGMGTNIRMG
                     EPSEIAELALFLASDKSSLVNGTVITADAGWTAY"
     gene            1543..1941
                     /locus_tag="VFU15_02265"
     CDS             1543..1941
                     /locus_tag="VFU15_02265"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEU4716622.1"
                     /translation="MKSSLLFFFLLAFASVAGAQKVCDTVTHVLSADSFKIMTGRHDG
                     VVLDVRTPDEFRTGYISGAVNIDFRAADFEAEISKLDKTKTYYVYCEVGGRSRMAADY
                     MTSHGFCQVYVLERGLRDWKQKGYPVTVPK"
     gene            1980..>4054
                     /locus_tag="VFU15_02270"
     CDS             1980..>4054
                     /locus_tag="VFU15_02270"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEU4716623.1"
                     /translation="MEDLPEQKGLNESEAKQLQVKFGFNELPSAAPKSIWKIIVGVLK
                     EPMFILLVGCGALYFVLGDPREGIMLFSSVTIIIAITFYQEKKTERALEALRDLSSPR
                     ALVIREGVTKRIAGREVVPGDVLILREGDRVAADAVVLLQTNLVTDESLLTGESVPVR
                     KSNWDRKQEFGPPQGEDIPFVYSGTLIVRGNGLARVVKTGTSTFIGKIGRSLKETQDE
                     PALLKAETRKIIRIFTITGFSLSATLVLVFGFIRHDWMGGVLEGISLAMAMLPEEFAV
                     VLTIFMALGAWRMSRRNVLTRNPASIETLGSVTVLCADKTGTLTENRMKVKKMFADGK
                     MWDDENGNGQAAQVFTELLHVARLASQQTVFDPMERAIADAQLQDGKSGAGDEGFVRE
                     YELSPALFAMTRVYRAAGKESFFAAAKGAPEAILSLCRVNAEERENILAQVNGFAKQG
                     LRVLAVARAEWKEKELPHAQKDFLFRFCGLLGFEDPLRKGVRENLQMCYDAGIRVIMI
                     TGDYPETAMHVAEKMGLKNADRVITGAAMEKMSDAQLGEDVRSVNVFARVVPEQKLRL
                     VNALKANGEIVGMTGDGVNDAPALKSAHIGIAMGQRGTDVAREAADIVLLNDDFDSVI
                     EAVRTGRSIYENIRKAMGYVFAVHIPIAGLAMLPVLFPSFPMILFPLHIAFLELIIDP
                     ASSLIFEAEP"
BASE COUNT          989 a          963 c         1220 g          882 t
ORIGIN      
        1 ctttccatgg cggtcgcaac gatggcctgc gcgttttacc cgcgcgaagt gatcgtgcgc
       61 atgagcgatt ttaagacgaa tgaatacgcc aacctcatcg gcggaaaaga attcgagcct
      121 gcggaagaaa accccatgat cggtttccgc ggcgcctcgc gttattacca tgaccgttac
      181 cgtgaaggct tccgcctgga atgccaggcg atgaaacgtg tgcgcgacaa catgggactg
      241 acgaatgtca agctgatgat cccgttctgc cgtacggtgg aggaaggcaa aaaagtgatc
      301 gcggaaatga agaagaatgg tctcgtgcgc ggaaagaacg gccttgaaat ttatgtgatg
      361 gctgaaattc cctcgaatat tctgctcgcg gaggaatttg cgaaaatatt cgacgggttt
      421 tccatcggct cgaacgacct gacgcagctc acgctcggca tcgaccgtga taattccgtt
      481 atcagcccgc ttttcaatga aaaggatgaa gcggcgaaac agctgatcgc aacgctcatc
      541 gcaaaagcga agaaagccgg tgtgaaagtc ggattgtgcg gccaggcgcc cagcgatttt
      601 cctgactatg cacagttcct cgtggaaagc ggcatcgaca gcatctcgtt caattcggac
      661 gcgctgatga acggcattgc caatatgaac aaagcagagg agctatctgc aaaaaagaaa
      721 aacgggagaa gcgcatcttc ttctattcgt aatcataaaa aacttcagaa tgaaaacact
      781 tgaaaacagg accgccgtta ttaccggcgc agcttccggc atgggaaaag ccatggcgga
      841 attgtttgcc tcggaaggag cgaacgtcat cgcagctgac cgcgatgaaa aagacctcgc
      901 ggaagtcgtg aaaatgatcc gtgaaaagaa aggaaatgtt accgctgttg tatgtgatgt
      961 aacgaaggag agtgatgtga agaacatgat cgacaaggcg gttgaaacct atggcggact
     1021 tgacattctc gtcaacaacg cgggtgtgct cgatgatttc atgcccgtgg cggaagtgag
     1081 cgatgatgta tggaaacggg tgatggatgt taaccttaac gggccatttt acgcctgcag
     1141 gcttgcggtt cctgtcatgc ttaaaaaagg aaaaggcagc attatcaacg ttgcttctgt
     1201 gggcggactt ttcggtgcgc gcgcaggagt tgcgtatacg acgtcaaagc atgccgtgat
     1261 cgggctgacg aagaacgtag gttacatgta tggcgacaaa ggcatccgtt gcaacgcgat
     1321 cgcgccgggt ggtgtgaata cgaacatcgg gaaaggaatg aacccgaacc cgctcggtta
     1381 tgcgaagctg agtctcggga tgggcacgaa tatccgcatg ggagaacctt cggagattgc
     1441 cgagctggct cttttcctgg catcggataa atcaagcctg gtcaacggca cggtcatcac
     1501 cgccgacgcc gggtggaccg cttactgaaa atccatgtca ggatgaaatc cagcctgttg
     1561 ttctttttcc tcctcgcgtt tgcatccgtt gccggtgcgc aaaaagtatg cgatacagtc
     1621 acgcacgttc tctcagccga ttccttcaaa ataatgaccg gccggcatga cggcgtggtg
     1681 ctggatgtgc gcacgccgga tgagttccgc actgggtata tcagcggtgc cgtcaatatc
     1741 gatttccgcg cagcggattt tgaagcggag atcagcaagc tcgataaaac aaaaacctat
     1801 tacgtgtact gtgaggtggg tggaagaagc aggatggcgg cagactatat gacttcccat
     1861 ggtttctgcc aggtatatgt gcttgaaaga ggtctgcggg actggaaaca gaaaggctac
     1921 ccggttacag ttccgaagtg aattgtttca tagcttacgg aaacgaataa ctgccttcta
     1981 tggaagactt gcctgaacag aaagggctga atgaaagtga agcgaagcag ctccaggtga
     2041 aattcgggtt caatgagctt ccttcggctg cgccgaaatc gatctggaaa attattgtgg
     2101 gggttctgaa agaacccatg ttcatcctgc tggtaggatg cggcgctctt tactttgtgc
     2161 tgggtgatcc gcgggagggc ataatgcttt tttcttccgt taccatcatt atcgcgatca
     2221 cgttttacca ggaaaaaaaa acggaacgtg cgctggaagc gctgcgcgat ctttcgagtc
     2281 cgcgtgcgct ggtgatccgc gaaggagtga caaaacggat agccggaaga gaagtggttc
     2341 ccggcgacgt gcttattctg cgtgaaggcg atcgcgtggc cgcggatgcg gtggtgcttc
     2401 tgcaaaccaa tctagtcacc gacgaatcat tgctgaccgg cgagtcggtt cctgtgcgta
     2461 aaagcaactg ggacagaaag caggaattcg gtccgcctca gggtgaagac atccctttcg
     2521 tgtattcggg aacgctcatt gtgcggggaa acggtcttgc acgcgtggtg aaaacaggaa
     2581 cgtccacctt catcggaaag atcggccggt cgctgaaaga aacgcaggat gaacccgcgc
     2641 tgctgaaagc agagacaaga aagatcatcc gcatttttac aattacaggt ttcagtcttt
     2701 ccgccacgct tgtgctcgtg ttcgggttca tccgccacga ctggatgggc ggcgtgctgg
     2761 aaggaatttc actggcgatg gccatgctgc cggaagaatt cgccgtggtg ctgacgatct
     2821 tcatggcgct cggtgcgtgg cgcatgtcgc ggagaaatgt gctgacgcgc aacccggctt
     2881 ccattgaaac gctcggcagc gtcacggttc tctgcgcgga taaaacggga acgctcactg
     2941 aaaaccggat gaaggtgaag aaaatgttcg cggacggaaa gatgtgggac gatgaaaacg
     3001 ggaacgggca ggcggcgcag gtattcacgg agctgctgca tgttgcgcgc ctcgccagcc
     3061 agcagacggt tttcgatccg atggaacgtg cgatcgccga cgcgcagctg caggacggaa
     3121 aaagcggagc cggcgatgag ggtttcgtgc gtgaatatga attatctcct gcgctgtttg
     3181 ccatgacgcg cgtgtatcgc gcagcgggaa aagaaagttt ttttgcagcc gcaaaagggg
     3241 cgcctgaagc gatcctttcg ctttgccggg tgaatgcgga agaacgtgaa aatattcttg
     3301 cgcaggtcaa cgggtttgcc aaacagggtt tgcgtgtgct cgccgttgcg cgcgcggaat
     3361 ggaaagaaaa ggaattgccg cacgcacaga aggatttctt gttccggttt tgcggcctgc
     3421 tcggtttcga agatccgctg cggaaaggcg tgcgggaaaa cctgcagatg tgctacgatg
     3481 cagggatccg tgtcattatg atcacgggcg attacccgga aacggcgatg cacgtggcgg
     3541 aaaagatggg actgaaaaat gcggaccgcg tgattaccgg cgccgcgatg gaaaagatga
     3601 gcgatgcgca gctgggagaa gatgtgaggt cggtgaatgt ttttgcgcgt gttgtgccgg
     3661 agcagaagct ccgtctcgtg aatgcgctca aggcgaacgg agagatcgtc ggcatgaccg
     3721 gtgacggtgt gaatgacgcg cctgcgctga aatcggcgca catcgggatt gcgatgggac
     3781 aacgcggaac ggacgtggcg cgggaagcgg ctgacatcgt cctgctcaat gatgatttcg
     3841 attcggtcat cgaggcggtc cgcacaggaa gatctattta cgaaaacatc cgcaaggcga
     3901 tgggatacgt tttcgccgtt cacatcccga tcgccggact cgccatgctg ccggtgctct
     3961 tcccttcttt cccgatgatc cttttcccgc tgcacatcgc attcctcgag ttgatcatcg
     4021 atccggcttc ttcgctgatc ttcgaggcgg aacc
//
LOCUS       DASTSF010000078         2357 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_618978,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000078 DASTSF010000000
VERSION     DASTSF010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2357
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_618978"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1062)
                     /locus_tag="VFU15_02275"
     CDS             complement(<1..1062)
                     /locus_tag="VFU15_02275"
                     /inference="COORDINATES: protein motif:HMM:NF021942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008239 - dipeptidyl-peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070009 - serine-type aminopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HEU4716624.1"
                     /translation="MKRLSLFFLFAFLFALKSFADEGMWLPVLLDQLNITDMKARGFQ
                     LNADDIYSVNHSSMKDAVCQFGGGCTAEVISGKGLVLTNHHCGFPYLQAYSTLDHNYV
                     RDGFWAMNAGQEIPCRGLTVTFIVSMEDVTAKILDGVQDTMSEASRNKVIDRNSKAME
                     KERSTKNSDAMVRAFYYGNVFYLFMTQTFKDIRMVGAPPNDVGDFGGDGDNWVWPRQT
                     ADFSLFRIYADKDNDPADYSPENVPYAPKYFFKINANGIGENDFTMVYGFPGHTTEYL
                     SSGGVSLIENVADPLKVDLRTKRIGIMEQQMAKSDSVKIKYAAKRNGAANAWKKWQGE
                     MKGLDDNHVIDQKKQDEAVF"
     gene            complement(1110..1826)
                     /gene="bshB1"
                     /locus_tag="VFU15_02280"
     CDS             complement(1110..1826)
                     /gene="bshB1"
                     /locus_tag="VFU15_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015691616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis deacetylase BshB1"
                     /protein_id="HEU4716625.1"
                     /translation="MPVDILAFGIHPDDVELSCSGTLLRHIQAGYSAGIIDLSQGELG
                     SRGNAELRLLEAEKAAKILGVSFRENLGMEDGFFGITRENKFRIIEKIRQHRPKIVLC
                     NALSDRHPDHGRAAQLVAESCFYSGLRKIETGFDKEMQEPWRPAAVYHYIQDHSLTPD
                     LVVDVTPYFEKKVEAINAFSSQFYDPSSKEPATPISSLEFMESVKAKMRVWGRVINVE
                     FAEGFNVSRTIGVDDLVKLK"
     gene            1905..>2357
                     /locus_tag="VFU15_02285"
     CDS             1905..>2357
                     /locus_tag="VFU15_02285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716626.1"
                     /translation="MKITLASLATLLLFSCTNENHSPVPQDSVHRDTAAFNPSSREGT
                     IRSLEKQHSTFIVSGKGDSLLGLYDDDTRILGDNSTIYSGKSGAKEYWTHAGDFYSLD
                     RNMVSLNGNKELLYEIGIETFKAHGTDSDYTGNQKYLVIWKLNTDGHYR"
BASE COUNT          538 a          648 c          596 g          575 t
ORIGIN      
        1 gaaaaccgct tcgtcctgtt tcttctggtc gatgacgtgg ttgtcgtcga gtcctttcat
       61 ctcgccctgc cattttttcc aggcgttcgc ggccccgttg cgtttcgcgg cgtattttat
      121 tttcaccgaa tcgcttttcg ccatctgctg ctccatgatg ccgatgcgtt ttgtacgcag
      181 atcgactttc agcggatcgg ccacgttttc gataagactg acgcccccgg aagaaaggta
      241 ttccgtcgtg tgaccgggga acccgtacac catcgtgaaa tcattttctc caatgccgtt
      301 tgcgtttatt ttgaaaaaat atttcggtgc atacggaaca ttttccggcg aataatccgc
      361 ggggtcgttg tctttatccg cgtaaatacg gaacagtgaa aaatccgccg tctggcgcgg
      421 ccacacccag ttatcgccgt cgccgccgaa atcgcccacg tcattgggcg gagcgcctac
      481 catgcggatg tctttgaacg tctgcgtcat gaaaagatag aacacgttgc cgtaatagaa
      541 tgcgcgcacc atcgcgtcgc tgttcttcgt cgaacgttct ttttccatcg ctttcgaatt
      601 cctgtcgatg accttattgc gcgaagcttc gctcatcgtg tcctgcacgc catcgagaat
      661 tttcgccgtc acgtcttcca tgctcacgat gaacgtaacc gtaagtcccc ggcagggaat
      721 ttcctggccg gcattcatcg cccagaaacc gtcgcgcaca taattgtgat cgagcgtgct
      781 gtaggcctgc aggtaaggga atccgcaatg gtgattcgtc agcaccaatc cttttcccga
      841 gatcacttcc gccgtgcatc cgccgccgaa ctggcagacg gcgtctttca tgcttgagtg
      901 gttaacactg taaatgtcat ccgcgttgag ctggaatccg cgcgctttca tatcggtgat
      961 attgagctgg tcgagcagca cgggaagcca catgccttcg tcggcaaacg acttcagcgc
     1021 gaaaagaaat gcaaaaagga aaaaaagaga aaggcgtttc attgcgggaa aaaattgaaa
     1081 agttgaaaag cggaaaagtt gggaagagat tatttcagtt tcacgaggtc gtccaccccg
     1141 atcgtgcgcg agacattaaa tccttccgca aattccacgt tgatcacacg accccacacg
     1201 cgcattttcg ctttgaccga ttccatgaac tcgagcgagg aaatgggcgt ggcgggttcc
     1261 ttcgacgaag gatcgtagaa ctgggagctg aacgcgttga tcgcttcgac tttcttttca
     1321 aagtatggcg taacgtccac caccaggtcg ggcgtcagcg agtgatcctg gatgtaatga
     1381 taaactgcag caggccgcca tggttcctgc atttctttgt caaaccccgt ttcgatcttt
     1441 cgcaggcctg aataaaaaca ggattccgcc acgagctgcg cagcgcgccc gtggtcggga
     1501 tgacggtccg aaagcgcatt gcagaggacg atcttcggcc ggtgctggcg tattttttcg
     1561 atgatgcgga atttgttctc ccgcgtgatg ccgaaaaaac catcttccat gccgaggttt
     1621 tcacggaaag atacgccgag tattttcgcc gctttttccg cctccagcag gcgcagctcc
     1681 gcatttccgc gggagcccag ctcaccctgt gaaagatcga tgatgccggc cgaatagccg
     1741 gcttgaatgt ggcgcagcaa cgtgccggag cagctgagct ccacatcgtc gggatggatg
     1801 ccgaatgcga gaatgtctac aggcatggtg attttttttc agaccgttga aacaggtccg
     1861 ccgcaaaatt agaaatcctt tgccgtgttt cgtttctttg cagaatgaaa atcacgcttg
     1921 cttccctcgc aacactcctt cttttttcct gcacgaatga aaaccattct cccgttccgc
     1981 aggattccgt tcatcgcgac acggccgcgt tcaatccttc ttccagggaa gggacgatcc
     2041 gctcgctcga aaagcagcac agcacgttca tcgtttcggg caaaggcgat tcgctgctcg
     2101 gactttacga cgatgacacg cgcatacttg gcgataattc cacgatctat tccgggaaaa
     2161 gcggcgcgaa agaatactgg acacacgcgg gcgacttcta ttccctcgac cgcaacatgg
     2221 tttcactgaa cgggaacaaa gagctcctct acgagatcgg catcgaaacc ttcaaagcgc
     2281 acggaacgga ttccgattac accggcaacc agaaatacct cgtgatctgg aaactgaata
     2341 cagacgggca ttaccgt
//
LOCUS       DASTSF010000079         2861 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_841111,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000079 DASTSF010000000
VERSION     DASTSF010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2861
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_841111"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1046
                     /locus_tag="VFU15_02290"
     CDS             <1..1046
                     /locus_tag="VFU15_02290"
                     /inference="COORDINATES: protein motif:HMM:NF040138.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HEU4716627.1"
                     /translation="SKTYGSPAMGQYQFSAPSVFSCVVGNTVYITSDQYDGTHSDILL
                     IAMDLDGKVACSPGTSLNVVQSTNSPSTTACNMTFSQPNLAYVPATGSGTRTFPDPCS
                     AYHVDLGPDTSSCSAFLLDAGNPGATYQWQDGSTQQTFTADGAGIYWVNVTINCCWIT
                     DSITVSYGTPLATLGGNAFICDGQEASLTASGGTSYSWSTGDTTATISIDTPGTYSVV
                     VSNQCGSDTLFSEVQSSSVTAAFAPGGNLAGGSFTAAFTDSSTNAVSWLWDFGDAAGS
                     ESSLQNPDFTYSADGCYPVTLTVTNDAGCMDTAVQGKGGKLCQIHTYVWKLSVTDVRG
                     EEHNLIGQVNLIR"
     gene            1135..>2861
                     /locus_tag="VFU15_02295"
     CDS             1135..>2861
                     /locus_tag="VFU15_02295"
                     /inference="COORDINATES: protein motif:HMM:NF024974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HEU4716628.1"
                     /translation="MRSAMHRKIHNAAFLLAVFFLLAPGIKAQTFFRTFGQPSLDECG
                     TVVIPSPDGNLFVAGYASDSALIMKVSPNGNVIWTRTFLPYDSASYINVIYYMTISPD
                     NYLVGTGSAECNSPYDRKGFFFRFDLNGNMQWCSRVNDTRPVYCERIMANSVSQYVLI
                     EDIAYLSNGTYNDVVTARVDASNGNVTWTSDRINYVSSNPYIDDVSSMTTGRNNAMYT
                     TGRIYIDGSFTDGMRPYVTKFDNTGNVLWSQYELFTFSQSATIYGEDLIYNNDSIVIC
                     YRGSRTATLGAYTNGLIVTDTLGNMLWSKDYDITSSPCEIANKVLKTPNGYLLIGFTN
                     EFGQLTTRHIFVIATDNQGNLLWSKTFGSNASGQYQYSVPTNFATLIGNTVYFTGVQT
                     DGTHSDLILASMDLDGNSSCSDAAPINVVESPNANATTTCSMSFYPSGLTYTTTSGTG
                     THPLPDECSFYHVDLGNDTDTHDPLLLDATVAGATYEWQDGSTQSTFTASAPGTYWVN
                     VNINCCRITDTIHLLDSYSTLNVPNVFTPNGDGTNDFFLITTEGIAALDVKIYDRWGM
                     FMNEITDPA"
BASE COUNT          672 a          820 c          702 g          667 t
ORIGIN      
        1 ggtcgaaaac ctatggctct cccgcgatgg gacaatacca gttttccgca ccctcggtat
       61 tttcctgcgt ggtcggcaat actgtttata tcacgagcga ccagtacgac gggacacatt
      121 ccgatattct gctcatcgcg atggacctgg acggtaaagt ggcctgcagc ccgggcacca
      181 gcctgaacgt cgtgcaaagc accaactctc cttcgacgac ggcgtgcaac atgacttttt
      241 cgcaacccaa cctcgcttat gtgccggcca caggatcggg cacacgcact ttccctgatc
      301 cctgcagcgc ctaccacgtc gacctgggtc cggacaccag cagctgttct gcttttttgc
      361 tggatgcggg gaaccccgga gccacttacc aatggcagga tggctcgacg cagcaaacat
      421 ttaccgcgga cggcgcaggt atttactggg tgaatgtcac tattaattgc tgctggatca
      481 ccgacagcat caccgtctcc tacggaacgc cgttggcgac cctgggcggg aatgctttca
      541 tctgcgatgg acaggaagca tcgctcaccg cctccggcgg cacctcttat tcgtggagca
      601 ccggcgacac gaccgccacg atcagcatcg atacacccgg aacctattct gtcgttgtca
      661 gcaatcaatg cgggagcgat actctttttt cagaagtcca gtcttcgtcg gtaacagctg
      721 catttgcacc tggcggcaac ctggcgggcg gttcattcac ggctgcattc accgacagca
      781 gcacgaacgc cgtttcctgg ctgtgggatt tcggcgatgc ggcgggatcg gaatcttcgc
      841 tgcagaaccc ggattttacg tacagcgctg acggatgcta cccggtaacg cttaccgtca
      901 ccaatgacgc cggctgcatg gatacggccg tgcagggaaa aggaggaaaa ctttgccaga
      961 tccatacgta cgtgtggaaa ctttccgtga cggatgtgcg cggtgaagag cataatctca
     1021 tcggccaggt caatctcatc cgctgagcct cctccccgtt cacgggcaac ccaaaatgtt
     1081 tgatattatc ccgcccggaa aaatgcttca ttacaataat taaggatcat gccaatgcga
     1141 tcagctatgc accggaaaat tcataacgcc gctttccttc ttgccgtttt ttttctgctt
     1201 gctcccggga taaaagcgca gaccttcttc aggacattcg gtcagccctc cctcgacgaa
     1261 tgcggcacag tcgtaatccc ttctccggac ggcaatcttt tcgtcgccgg ttatgcttcg
     1321 gacagtgcgc tgatcatgaa agtctcaccg aatggaaacg tgatctggac ccggactttt
     1381 cttccttacg attccgcctc ttatatcaat gtgatttatt acatgaccat ttcaccggac
     1441 aattacctgg taggaacggg ttctgccgag tgcaacagcc cttatgacag gaaaggtttc
     1501 tttttcaggt ttgatctgaa cggaaacatg cagtggtgca gcagagtgaa cgacacacgc
     1561 ccggtttatt gcgaacgtat catggctaat tctgtaagcc agtatgtgct gatcgaagat
     1621 atcgcctacc tgtcgaacgg aacctataat gatgtcgtga cggcaagagt ggacgcttcg
     1681 aatggcaatg tcacgtggac ctccgatcgc atcaattatg tctcatcgaa tccgtacata
     1741 gacgacgtga gttcaatgac cacggggagg aataacgcta tgtatacgac cggcaggatc
     1801 tacatcgacg gttcttttac agacggaatg cggccgtacg ttaccaagtt tgacaacacc
     1861 gggaatgttt tatggtcgca atacgagctc ttcactttca gccagagcgc aactatctac
     1921 ggagaagacc ttatctacaa caatgattcc atcgtgatct gttacagggg tagcagaacc
     1981 gcaaccctcg gtgcctacac taacggcctg atcgtgaccg atacgctggg gaacatgctc
     2041 tggtcgaagg attacgacat cacttcttcg ccctgcgaga tcgccaacaa agtgctgaag
     2101 accccgaatg gctatctgct catcgggttt acgaatgaat tcggtcagct tacaacacgg
     2161 cacatttttg tgattgccac tgacaaccag ggaaacctgc tgtggtcaaa gacgttcggc
     2221 tcaaacgcat ccgggcaata ccagtattcc gtccccacga atttcgcaac gctgatcggg
     2281 aataccgttt acttcaccgg cgtacagacc gacggaactc attccgacct tatactggct
     2341 tccatggacc tggatggcaa ttcatcctgt tccgacgccg caccgatcaa cgttgtcgaa
     2401 tctccgaatg caaatgcgac gacgacatgc agcatgtctt tttatccttc gggacttacc
     2461 tacacaacca catcaggtac gggcacgcac ccgcttcccg acgaatgcag cttttaccat
     2521 gttgatctcg gcaatgacac cgatacccat gatccgctcc tgctggacgc tacggttgcg
     2581 ggggcaactt acgaatggca ggacggctcc acgcagtcga cttttactgc gtccgccccc
     2641 ggcacttact gggtcaacgt caatataaat tgctgccgga tcacggacac gatccatctc
     2701 cttgattcct acagcacgct gaacgttcct aatgtcttca caccgaacgg ggacggcacg
     2761 aatgatttct tcctcatcac gacagaagga attgctgcgc tcgatgtgaa aatctacgac
     2821 cgctggggca tgttcatgaa cgagatcacc gacccggcgc a
//
LOCUS       DASTSF010000080         8718 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_880224,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000080 DASTSF010000000
VERSION     DASTSF010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 8718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8718
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_880224"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(194..1597)
                     /gene="rlmD"
                     /locus_tag="VFU15_02300"
     CDS             complement(194..1597)
                     /gene="rlmD"
                     /locus_tag="VFU15_02300"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007656794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEU4716629.1"
                     /translation="MLKNKNPVFEKVEITGIAAEGKAVGRAGELVLFVANAVPGDVAD
                     IRVTKKKKNFAEGVAVKFHTLSGKRSEPACPHFGTCGGCKWQHLSYEWQLKYKQQQVQ
                     DAFGRIGKIQFPEPLPILGSKKIYNYRNRLDFSFSDKKWLTLEEINSGVRVEDRNALG
                     FHIPGTFDKVLDLKECHLQGEISENIRRFVREYTGKSGYTYFDIRDHAGMMRSLIIRT
                     SSTGEVMVIVQFFDDDFELRDALLAAMAEKFPQITSLLYVINQKGNDTFNDLAVHTFS
                     GREYIIEEMEGLQFRVGPKSFYQTNSAQAYELYKVARDFAGLTGNENVYDLYTGTGTI
                     ALFVAGKAKKVTGVEIVEAAIADAKLNAELNGISNAGFFAADMKDIFNAAFLEEHGKP
                     DVIITDPPRAGMHEDVVKSICGSGAEKIVYVSCNPASQARDLSLLDPFYSVEKVQPVD
                     MFPQTHHVENVVLLKKR"
     gene            complement(1733..2287)
                     /locus_tag="VFU15_02305"
     CDS             complement(1733..2287)
                     /locus_tag="VFU15_02305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716630.1"
                     /translation="MTNRTLTALAIRFAALLLFMKIFDQFGFYFLSIYYTALMPVLEN
                     QLHTSLDKFYYNGTFLLLGNFLISGILFFKAEWVSSKLIKVDSDITIGLTPEKLIRVI
                     LLTTGITWLAYSVYLLPDLYDYIQMMIAKANHLGFAKKVDFSPIYYILKTTIAFLFVF
                     RVEKISSYLEKKMNRSEVRQDKNN"
     gene            complement(2314..3492)
                     /locus_tag="VFU15_02310"
     CDS             complement(2314..3492)
                     /locus_tag="VFU15_02310"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEU4716631.1"
                     /translation="MRNTKILVSGAGVGGLTVAYFLKQKGFTPVVIEKASSLRDGGYM
                     IDFFSSGISVAEKMGIIEQLKEKDHRSNIIIQQNEKGEKQFILNMSGFRESVKGKLFN
                     FLRTDLVDVLYKKVKEDVEIRFSTSIQSISQDSNNVTVTFENGKQETFDLLIGADGLR
                     SNVRAKVYAPEKIEELFLGCYVCGLEHNVPVNVDNGEVISMLCPKRQIMTYTTGKGVS
                     NSLFVFKSPKLKLLTTKEEIDVLNKEFAGFASPVPEIINEASKKDKLFFDQVTQIRIH
                     DQWHKNRVVLIGDAAFCITLLSGQGSSMAMTAAYVLSEQLEKHRDDYETAFTEYEKSL
                     RPMIEKMQKKAVGNSATFIPASKFSLWIRNLFAPLVFKKIFSSLLIKQLGANNYFEKE
                     "
     gene            complement(3485..4048)
                     /locus_tag="VFU15_02315"
     CDS             complement(3485..4048)
                     /locus_tag="VFU15_02315"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEU4716632.1"
                     /translation="MPRTDKQLEELRNAKTELIESVAMKCFAAKGFHATSISYIARKA
                     GISAGLMYNYFSGKDELLKSIYLKGIKKVFAPLEGEALTKEKFMAFIEYIFNEMETNT
                     EFWKLYFIVISQPDVLARYQAYMLETALPIMNAIISYFAKQRMKNPEVEAQFLFSTLD
                     GVCINYLINYPKYPLKQIKQKILKNYA"
     gene            complement(4270..8139)
                     /locus_tag="VFU15_02320"
     CDS             complement(4270..8139)
                     /locus_tag="VFU15_02320"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HEU4716633.1"
                     /translation="MFYRTSFRFLAMLFLLSGISMSAQTFWMQHAGSATIDEGMDVAV
                     DGSGNTFSTGYFTSTATFGSSSLSSSGVDDIYLARLGTNGLYQWIQKAGGTGSDRALS
                     VKADAAGNSYITGFFHGTATFGSQSVTAAGAQDVFIAKYDPSGTCLWVKSGGGANADI
                     GNGITVDNSGNVIVTGEFAGTATFGSFSLTSMNGSTDVFTTKLDASGNFLWAKKGTAP
                     LTDRGIDVDCDASGNIYVTGQFSDSITFDVTHNNNMLNAIFLISYDPSGNEQWFRVIG
                     GGVVNISNSIACNGAGGIFLTGDFQGTLTFFGSTNTNLSATYANCIFIARYDQSGNLV
                     WDAAESSDSPLTSRSITADANSCFIAGNFKCTFDSYSDRYGSGIFNSSGYWDIFEGKY
                     DASSGSWGMSRQLGGKKDQNCNGIAVDAAGNPHVTGSYVQSLVVPVDPSFYGFIFFTG
                     YGMTMNTDVATSTVYCSDPYYNSYAVAPAVGNADIFIGDPFDPARAPYDFYWRNGSGC
                     VTPWVDLCIHRQNNYDYDCFGDTLDACTVAHLMAATQTSLFSPANSVGPDFTYQWSNG
                     ATSPTTNISTTGYCWATITSADGCYTREDSIYVIIHPPPPIPTISDNVGINNNSTNPQ
                     LVEICGDSVILTGGNLGSNTYGWTGPQFDPSFSGSNPVTIDSSGNYVLTVVDQYGCKN
                     TDTVPVLLDHPLQQIIPDIYCLDDSDQNDSITVCDGTNIHFMPYDSLTNPNALPECID
                     QLTVVAWHISPGNPNAIISDSTDCQSILNAISTAIVHQTGWYTITEIIIRNNLCGNDT
                     VTYSHNYYFDILPVPPPGSLPMVITGDSLFCPGDSAMLVVTGGANYLWNTNSTNDTIY
                     TTLPGNYYVQGIDTVTNSYGCSTVYYGYANAVVNMTPQPVVTSLPADGTICPGDSVEL
                     MSTGNGNFAWNGPNGPIATNSSSIWVTSPGTYYCIVTDPSGCTLLSNSVILQQYNTPG
                     LSLSPSSVLCPGDTVVLTVNSNNGSTITWLPPLSGNSLTQYITQPGTYSCSIQSCSIP
                     TNLSVTIVPAQAVAMITPLTSTVACEGDSILLGANTGMNTYNWQPGNSSALTIYAHNS
                     GMYYLTTTDTGGCVARDSFAVQFTQNLLQPPAVTDTTICAGKPVLLIAGGTPVINWYL
                     TPSGGTPFTTGPYLQLPAVFADTAFYLLSDDSVCRSGYSVIRVHTEECTPLTPNVFTP
                     DGDGVNDVWALYEPYAAGIRVRIYDRWGVLVYEYGDLDGYWDGTYMKNGKPVTDGVYY
                     YIGDVDMGSGNVEQKTGFVQLIRNGGK"
     gene            8273..>8718
                     /locus_tag="VFU15_02325"
     CDS             8273..>8718
                     /locus_tag="VFU15_02325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716634.1"
                     /translation="MAFLASILLLCVLFFLPLSQKRSLAFFFAAAGILLWLFFVSAAG
                     FALFCEPHPLSDLQNRSSLPGLKEILAADSENIYVTTTVNSASASGDSAGNETETWLE
                     TKRIGDFKTIFSRKVGECNTLNAITSAATDGKRLVLTFSSFRQGQG"
BASE COUNT         2055 a         2162 c         2240 g         2261 t
ORIGIN      
        1 gatcacggga aaagccctgt aggtgcagca ggaccgcaaa caataccgta aaatatttta
       61 aaccgttccg caagggagct gttttaataa tacactaagt taagaaaatt ccgttgctgc
      121 aaacttcatg cccccgggcg gtcattagga taaacgaacg gcattagcaa ggtttaacaa
      181 ataaaggagc ggatcacctc tttttcagca gcacgacgtt ctccacgtgg tgggtctgag
      241 ggaacatatc caccggctgc actttttcca cggaatagaa cgggtcgagg agagaaaggt
      301 cgcgcgcctg cgaagcggga ttgcagctga cgtagacgat cttttccgcg ccgcttccgc
      361 agatcgactt cacgacgtct tcgtgcattc ccgcgcgcgg gggatcggtg atgatgacgt
      421 cgggttttcc gtgctcttcc aggaaagctg cattgaaaat gtctttcatg tcggcggcga
      481 aaaagccggc attggaaatt ccgttcagct ccgcgttcag ctttgcgtcg gcgatggccg
      541 cttccacgat ctcgacgccg gtcacttttt tcgctttccc tgccacgaaa agggcgatcg
      601 ttcctgttcc ggtgtagagg tcgtacacgt tttcattccc ggtaagtcct gcgaaatcgc
      661 gcgccacttt gtacagctca tacgcctgcg cggaattcgt ctggtaaaat gatttcggtc
      721 cgacgcggaa ctgtaatcct tccatttcct cgatgatgta ctcgcggccg gagaacgtgt
      781 gcacggcaag gtcgttgaac gtgtcgttcc ctttctggtt gatcacgtac agcagcgagg
      841 tgatctgcgg gaatttttcc gccatcgcag caagcagcgc atcgcggagc tcgaaatcat
      901 cgtcgaagaa ctgcacgatc accatcactt cgccggtgga ggaggtgcgt atgatcagcg
      961 accgcatcat cccggcgtga tcccggatat cgaaatacgt gtagccgctc ttccccgtgt
     1021 attcgcggac gaacctgcgt atgttctccg aaatttctcc ctgcaggtgg cattccttca
     1081 ggtcgagcac tttatcgaac gtcccgggaa tgtgaaaccc cagcgcgttg cggtcttcca
     1141 cccggacgcc gctgttgatc tcttcgagcg tgagccattt tttatcggaa aaggaaaaat
     1201 cgaggcggtt gcggtaattg taaatttttt tcgagccgag gataggaagc ggttccggga
     1261 attgaatttt cccgatgcgc ccgaatgcat cctgcacctg ctgctgcttg tacttcagct
     1321 gccactcata ggaaagatgc tgccatttgc agccgccgca cgttccgaaa tgcgggcacg
     1381 ccggttcgga acgtttgccg gaaagcgtat gaaatttcac ggccacgcct tccgcaaaat
     1441 tctttttctt tttcgtgacg cgtatgtccg ccacgtcgcc gggaaccgca ttcgccacga
     1501 acagcacgag ctccccggca cgcccgacgg cttttccttc agcagcaatg ccggtgatct
     1561 caactttttc gaaaacaggg tttttatttt tcagcacgct gcgaaaatag gaatatagat
     1621 tttcacctca acgcagcgag agaaactatc cggtggatgg agcggaaggg tctttgatgt
     1681 cccgcggttt aaccggacac ttggaacaag tgcccggttt tcagtaaaac cctcagttgt
     1741 ttttatcttg tctgacttcg ctcctgttca tttttttctc aagatacgaa ctgatttttt
     1801 caacccggaa aacaaaaagg aaggcgatcg ttgtctttag tatataatag attggactga
     1861 aatccacctt ctttgcaaac ccaagatgat tggcttttgc aatcatcatt tgaatgtagt
     1921 cgtaaagatc gggaagcaaa taaaccgaat acgcaagcca ggttattccg gttgtcagga
     1981 gtatgaccct gattaatttt tcaggcgtca gcccgatagt aatgtcactg tccaccttaa
     2041 tgagctttga tgaaacccat tccgctttaa aaaacaaaat acctgaaatc aagaaattgc
     2101 caaggagtaa gaacgttccg ttgtagtaaa atttgtcaag cgaggtatgt aactgatttt
     2161 ccaaaaccgg cattaacgct gtataataaa tagaaagaaa ataaaaaccg aactggtcaa
     2221 atattttcat gaagagtaat agcgcagcaa atcttattgc cagggctgtt aaggttctat
     2281 tggtcattgg cgggttctga ttttattgtc cggttattcc ttttcaaaat agttatttgc
     2341 ccccaattgc tttattaata agctggaaaa tattttttta aatacgagcg gggcgaaaag
     2401 attcctgatc caaaggctaa acttcgatgc cggaataaat gtcgctgaat tgccaaccgc
     2461 ctttttttgc attttttcaa tcattggccg gagtgatttt tcatattctg taaatgctgt
     2521 ttcataatca tccctgtgct tttccagttg ctcggaaaga acataagcag cggtcattgc
     2581 cattgaagaa ccttgcccgg aaagcagggt aatgcagaat gccgcatcac cgatcagcac
     2641 caccctgttt ttgtgccatt ggtcgtgaat acggatttgg gttacctggt caaagaaaag
     2701 tttgtctttt ttggatgctt cgttaattat ttcaggcacc ggggatgcaa aaccggcaaa
     2761 ctctttattt aaaacatcta tctcttcctt cgtagttaaa agtttgagtt tgggagattt
     2821 gaatacaaaa agactgtttg aaactccttt gccggtggta tacgtcatta tttgcctttt
     2881 aggacaaagc attgagatga cttccccgtt atccacgtta accgggacat tgtgctccag
     2941 tccgcagacg taacagccaa gaaacaactc ttcaattttt tcaggggcat atacttttgc
     3001 acggacattg gacctaagcc catcggcccc gataagtaaa tcgaaggttt cctgtttccc
     3061 gttttcgaag gtcacggtaa cgttattact atcctggctt atactttgta tggaagtcga
     3121 aaacctgatt tccacgtctt ctttgacttt tttataaagc acatccacta aatccgtgcg
     3181 taaaaagttg aacaatttcc ccttgacaga ttcccgaaaa ccggacatgt tcaaaatgaa
     3241 ttgtttttca cctttttcat tttgctgaat gatgatgttg gaacggtggt ctttttcttt
     3301 taactgctca atgatcccca ttttttcagc tacggaaatt ccggacgaga aaaagtctat
     3361 catgtaaccg ccgtctctca gggatgaggc tttttcaatg accacggggg tgaacccttt
     3421 ttgctttagg aagtaggcga ccgtaagtcc gccgacgcct gcacctgata ccaggatttt
     3481 agtgttacgc ataattttta agaatttttt gtttgatctg ctttaaagga tatttcgggt
     3541 agttgattaa ataattgatg catacgccat ccagtgtgga aaacaggaac tgcgcctcca
     3601 cttcagggtt tttcatcctt tgcttagcaa aatagcttat gatggcgttc ataatcggca
     3661 aagctgtttc caacatgtac gcttgatagc gggccaatac atcgggttgg ctgattacaa
     3721 tgaaatacag tttccagaat tcagtgttgg tttccatttc attgaaaata tattcaatga
     3781 aagccatgaa cttttctttg gtaagtgctt ccccttccag tggcgcaaat acctttttta
     3841 tgccttttaa gtagatggat ttgagcagtt cgtctttacc tgaaaaatag ttatacatca
     3901 ggcccgcaga aatgcccgct tttctggcaa tgtagcttat cgaagtcgcg tgaaatcctt
     3961 tggcagcgaa acatttcatc gctacacttt cgatcagttc tgttttcgcg tttctaagtt
     4021 cctcaagctg cttgtccgtc ctcggcatat tttatgattg aatgttcatt caaaggtaaa
     4081 taaataaaaa aacaccaaag tcgcggagat aaattttttt accctgtgtt aagatgtgtt
     4141 tttgaaatag aggctaacgc ctggcagatc tccgaaaacc gggaattacc attcgcaaac
     4201 gatggttttc acgcgccgtt caacgttgct gtatgaagct gcctgtcccg cccgggatcg
     4261 aagaccaggt tattttccgc cgttcctgat cagctgcaca aacccggttt tctgctccac
     4321 gttcccgctt cccatatcca catcgccgat gtaataataa acgccgtctg ttacgggttt
     4381 tccgttcttc atgtacgtcc cgtcccaata gccgtccagg tcgccgtatt cgtacacgag
     4441 cacgccccag cggtcgtaga tgcgcacgcg tatgcctgcc gcgtaaggtt cgtacagcgc
     4501 ccacacgtcg ttgacgccgt caccgtcggg cgtgaaaaca tttggcgtaa gcggcgtgca
     4561 ttcttcggtg tgaacgcgga tcacggaata accgctgcgg cagacgctgt catcgctgag
     4621 aagatagaac gccgtatctg caaacacagc cggaagctgg agataaggtc ccgtcgtgaa
     4681 aggagttccg cccgaaggag tgaggtacca gttgatcacc ggtgttccgc ccgcaatgag
     4741 aagtaccggt tttcccgcgc agatcgttgt gtcggtcacc gccggcggct ggagcaggtt
     4801 ttgcgtgaac tgtacggcga acgaatcgcg cgcaacgcaa cctccggtat cggtggtggt
     4861 gaggtaatac atcccgctgt tgtgtgcgta gatcgtcagc gcggagctgt ttcccggctg
     4921 ccagttgtat gtgttcatcc cggtgttggc gccgagaaga atggaatcgc cttcgcaggc
     4981 aacggtggat gtcagcggtg tgatcatcgc cacggcctgc gccggaacga tcgttacgga
     5041 aagattcgtg ggaatgctgc acgactgtat agagcaggaa taggtgcccg gctgcgtgat
     5101 gtattgcgtg agcgaattcc cgctgagcgg cggaagccac gtgatggtgc tgccgttgtt
     5161 gctgttcacg gtaagcacga cggtgtcgcc gggacaaaga acagaggacg gtgaaaggga
     5221 aagcccgggc gtgttgtatt gctgcaggat aacggaattg gacagcagcg tgcatcccga
     5281 cggatccgtg acaatgcaat aatacgttcc gggcgatgtc acccagatgc tggatgagtt
     5341 cgtcgcgatg ggaccgttcg gtccgttcca cgcgaaattt ccgttgcccg tcgacataag
     5401 ttccacggaa tcgccggggc agatcgttcc gtcagcggga agtgaagtca cgactggctg
     5461 cggcgtcatg ttcaccacgg cattggcata gccgtaataa acagtggagc aaccgtagct
     5521 gttggtaacg gtgtcgattc cctgcacgta ataattgccg ggcagcgtgg tgtagatggt
     5581 gtcgttcgtg gagttggtgt tccataaata attcgcaccg ccggtaacaa cgagcatcgc
     5641 ggaatcgccg ggacaaaaaa gcgaatcgcc tgtgatcacc atgggtaatg atcccggggg
     5701 aggaacgggc aggatgtcaa aatagtagtt gtggctatac gtcaccgtgt cgttcccgca
     5761 caggttgttc cgtatgatga tttccgtgat ggtataccat cctgtctggt gaacgatcgc
     5821 tgtgctgatc gcgttgagta tggactggca gtccgtggaa tcggaaatga tcgcattggg
     5881 atttcccggc gagatgtgcc acgccacgac ggtaagctga tcgatgcatt cgggtaatgc
     5941 attcggattc gtgagcgaat catacggcat gaaatgaatg ttcgttccgt cgcatacggt
     6001 gatgctgtcg ttctggtcgg aatcatcgag gcagtaaata tccgggatga tctgctgcag
     6061 tggatgatcg agcagcacag ggacggtgtc cgtgtttttg cagccgtact ggtcgacaac
     6121 cgtaagcaca tagttgcccg agctgtcgat cgttaccggg ttcgagcctg agaaagaagg
     6181 atcgaattgc ggaccggtcc agccataggt attggaaccc aggtttccgc cggtgagaat
     6241 gacggaatct ccgcagatct ccaccagctg cggattggtg gaattgttgt ttatcccgac
     6301 gttgtcgctg atcgtcggaa tgggcggcgg cggatgaatg atgacgtaaa tggaatcttc
     6361 ccgtgtatag cagccgtccg ccgaagtgat cgtcgcccag caatagccgg ttgtgctgat
     6421 attggtcgtc ggcgaagtag ctccgttcga ccactggtag gtgaaatccg ggcctacgct
     6481 gttcgccggt gaaaacagcg atgtctgcgt ggccgccata agatgcgcaa ccgtgcaggc
     6541 gtcgagcgta tcgccgaagc aatcgtaatc gtaattattc tgccggtgga tgcagagatc
     6601 cacccatggc gtgacgcagc cgcttccgtt ccgccagtaa aaatcatacg gagcgcgggc
     6661 cggatcgaac ggatcgccga taaaaatatc cgcattcccc acggccggtg caacagcata
     6721 ggaattgtaa tacgggtcgc tgcagtaaac agttgacgtg gcgacgtctg tgttcatggt
     6781 cattccatag ccggtgaaaa aaataaaccc gtaaaacgaa ggatcgacag gcacgacaag
     6841 cgattgaacg taggaacccg ttacatgcgg gtttcccgcg gcatccacgg cgatgccgtt
     6901 gcagttctgg tctttttttc cgccgagctg ccggctcatg ccccatgaac cggaggatgc
     6961 gtcgtatttc ccttcgaaaa tatcccagta gcccgagctg ttgaatatgc cgcttccgta
     7021 acggtcgctg tagctgtcga acgtgcattt gaaattcccg gcgatgaagc agctgttcgc
     7081 atccgccgta atgctccgcg aggtcagcgg tgaatcggag ctttctgcgg cgtcccacac
     7141 gaggtttccc gactggtcgt agcgggcgat gaagatacag ttggcataag tcgcggaaag
     7201 attcgtgttc gtgcttccga aaaaggtaag cgttccctgg aaatcgccgg tgagaaaaat
     7261 tcccccggcg ccgttgcagg cgatcgagtt ggagatgttt accacgccgc cgccgatcac
     7321 gcggaaccac tgctcgttgc ccgacgggtc atagctgatg aggaagatcg cgttcagcat
     7381 gttgttgttg tgggtcacgt cgaacgtaat gctgtcggag aattgcccgg tgacatatat
     7441 atttcccgaa gcgtcgcagt cgacatcgat gccgcggtcg gtgaggggag ccgttccttt
     7501 tttcgcccag agaaaattcc ccgacgcatc gagcttcgtg gtgaaaacgt cggtgctccc
     7561 gttcatgctc gtcagtgaaa agctcccgaa cgtcgcggtg cccgcaaatt ccccggtgac
     7621 gatcacattt cccgaattat ccacggtgat cccgttgccg atatccgcat tcgcgccgcc
     7681 cccgcttttc acccacaggc acgtacccga cggatcgtat ttcgcaatga aaacatcctg
     7741 tgcgcccgcc gccgtcaccg actggctgcc gaacgtcgcc gttccgtgaa aaaaacccgt
     7801 gatgtagctg ttgcccgctg catcggcttt taccgataac gcacggtcgg atcccgtacc
     7861 gccggctttc tgtatccact ggtaaagtcc gttcgttccc agcctggcga ggtaaatgtc
     7921 atccacgccg gaagagctta aagacgagct gccgaacgtt gcagtggagg tgaaatagcc
     7981 cgttgaaaat gtgttccccg atccgtcaac agccacgtcc attccctcgt cgatcgtcgc
     8041 gcttcctgca tgctgcatcc agaatgtctg tgcagacatg gaaatgccgg aaagcaaaaa
     8101 caacatcgcc aggaagcgaa aagaggttct gtaaaacatc tgaaatactg tttgccaatg
     8161 gaaagataat tctattcttg ttaaaaagaa cctttgccgt tgtttgcggc tgcttttttt
     8221 atcacaatca ttaaacagat tttatccgca attcccaaaa tccatgaacc ccatggcctt
     8281 cctggcatcc atcctgctgc tgtgtgtgct tttttttctg cccctgtcgc agaaacgttc
     8341 gctggctttt tttttcgcag cagccggtat cctgctgtgg ctgttctttg tttccgcagc
     8401 cggttttgcg cttttctgcg aaccacatcc gctaagcgat ttgcagaaca gaagttcttt
     8461 gcccgggctg aaagagatac tcgccgccga cagcgaaaat atttacgtga ctacaactgt
     8521 gaacagcgct tccgcatccg gcgattctgc gggcaatgaa acggaaacct ggctggaaac
     8581 aaagcgtatc ggtgatttca aaacgatctt cagccggaag gtcggtgaat gcaatacgct
     8641 taacgcgatc acttctgcag caacggacgg aaaacggctt gtgctgactt tttcttcttt
     8701 ccgccagggg caggggaa
//
LOCUS       DASTSF010000081         3140 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1733944,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000081 DASTSF010000000
VERSION     DASTSF010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3140
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1733944"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..839
                     /locus_tag="VFU15_02330"
     CDS             <1..839
                     /locus_tag="VFU15_02330"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008242802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HEU4716635.1"
                     /translation="AIPLMRGKHVSTPIEQLVAQAKNLTKNGTKELILIAQDSTYYGL
                     DIYGKRNLSELLKHLADVEGIDWIRLHYAFPSGFPMDILDVMRETPNICNYIDMPLQH
                     ISDHMLKSMKRGTTKQKTIDLVNEVRDRVPGITFRTTLITGYPGETEKDHEEMKEWVE
                     QTRFDRLGVFTYSHEENTSAYKLNDDVPAEIKQQRAEDVMEIQSGISAEINRTKEGKT
                     FKVLFDRKEGDYFIGRTEADSPEVDNEVLVSAKDNYVRTGDFASVKIERTEEFDLYGV
                     VC"
     gene            971..2572
                     /locus_tag="VFU15_02335"
     CDS             971..2572
                     /locus_tag="VFU15_02335"
                     /EC_number="1.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008510062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HEU4716636.1"
                     /translation="MRKPFIYFSIFLIAALAAATYFWHPAAWSFVLVLPVILVGVIDM
                     NQEHQTIRRNFPVFGRMRYLAEWMRPKIYQYFIESDTDGTPFNRMSRSVVYQRSKKVT
                     DTTPFGTQLNVYETGYEWLNHSIAPLSAHDLEENPRVIVGGPECKQPYAASILNISAM
                     SFGSLSQNAILALNGGAKLGGFAHNTGEGGLSPYHLRPGGDIIWQIGTGYFGCRNKDG
                     TFNYELFAERAVLPNVKMIEIKLSQGAKPGHGGILPARKVTEEIAKIRLVEMGKDVLS
                     PPGHTAFRTPVEMMHFIKKLRDLSGGKPVGFKLCVGNKGQFLALCKAMVKTGIMPDFI
                     TVDGGEGGTGAAPLEFSNHVGMPLRDAVAFVYDALNGFALKRHIRIIASGKVSSGFEI
                     VKNLALGADMCNSARAMMLALGCIQALECNTNTCPTGVATQDKSLMKGLVVDDKKVRV
                     MNFHNETVKSAVELMAAAGIDKPGKLHRSHIYRRISPGEIRTYAETYPYILRGCLLEP
                     PFPAQYELDMANCHEETFESAIQYA"
     gene            2584..>3140
                     /locus_tag="VFU15_02340"
     CDS             2584..>3140
                     /locus_tag="VFU15_02340"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEU4716637.1"
                     /translation="MNTSLLEDEFRSKVSIPDGEFEKFSALLESFSIRRNEHFFQAGD
                     VVKFAVFILKGCLRQYYISPEGVESTVYFAEEKWWSGDASSFRNATPTNLNLQAMEDC
                     EVLLLSRKNWQYAFDTFAWYREYHTKGQQRLAARMQEKLGQALTETAEQKYLRLLKER
                     PSLIRRVPQHYIATYLGLTPETLSRV"
BASE COUNT          759 a          863 c          870 g          648 t
ORIGIN      
        1 gtgccatccc gctcatgcgc gggaagcacg tttccacgcc catcgaacag cttgttgcgc
       61 aggcgaagaa cctcacgaag aacggaacga aagagctcat tctcattgcg caggattcca
      121 cgtattacgg cctcgatatt tacgggaaac gcaacctcag cgaactgctg aagcatcttg
      181 cggacgtgga aggcatcgac tggatccgcc tgcattacgc gttccccagc ggtttcccga
      241 tggacattct cgacgtcatg cgcgaaacgc cgaacatctg caattacatc gacatgccgc
      301 tgcagcacat cagcgaccac atgctcaaaa gcatgaagcg aggcacgacg aagcagaaaa
      361 cgatcgatct cgtgaacgag gttcgcgacc gcgtgccggg aattacgttc cgcacgacgc
      421 tcattaccgg ttatcccggc gaaacggaaa aagaccacga ggaaatgaag gaatgggtgg
      481 agcagacgcg tttcgaccgc ctcggcgtgt tcacgtattc gcacgaggaa aacacttcag
      541 cgtacaagct caatgacgac gttcccgcag aaataaaaca gcagcgcgct gaagatgtga
      601 tggaaattca gtcagggatt tccgcggaga tcaaccgaac gaaagaagga aagacgttta
      661 aagttttatt cgaccgcaag gaaggcgatt atttcatcgg ccgtaccgaa gccgattcgc
      721 ccgaagtgga caacgaggtg ctcgtttccg ccaaagataa ttacgtgcgc accggcgatt
      781 tcgcgagcgt gaagatcgaa agaacggaag agttcgacct gtacggcgtc gtttgctgag
      841 ttcccggagg gcaggcgcgg tttgtgattt ttcgagttgc gttctggccc ggtaaagcgt
      901 cattatcttt ttccgggaga cgtttttttc gcatcaatcg cttaactttc ttttctcaaa
      961 tccgctcgcc atgagaaaac cattcattta cttttccatt tttcttattg ctgcgctggc
     1021 agccgccact tatttctggc atcccgcggc gtggtcgttt gttctcgtgc ttcccgtaat
     1081 tctcgtcggc gtgattgaca tgaaccagga acaccagacg atacggcgca acttcccggt
     1141 attcggaaga atgcgttacc tcgcggaatg gatgcgaccg aaaatctacc agtacttcat
     1201 cgaatccgac accgacggca cgccgttcaa ccgtatgagc aggagcgtgg tttaccagcg
     1261 ttcaaaaaaa gtgacggaca ccacgccgtt cggtacgcag ctcaatgtgt acgaaaccgg
     1321 ttacgaatgg ctgaaccaca gcattgcccc gctgagcgca cacgacctcg aagaaaaccc
     1381 ccgtgtgatc gtcggcggac ctgaatgcaa acagccgtat gcagcgagca ttctcaatat
     1441 ttctgccatg agtttcggtt ccctcagcca gaatgcgatc ctggcgctca acggaggcgc
     1501 gaagctcggc ggttttgcgc acaacacggg cgaaggcggg ctcagtccgt atcacctccg
     1561 gcccggcggc gatatcatct ggcagatcgg tacgggttat ttcggctgcc gcaacaagga
     1621 cggcacattc aactacgaac tgttcgccga acgcgccgtg ctgcccaatg tcaagatgat
     1681 agagatcaaa ttgtcgcagg gcgctaaacc gggtcatggc ggcattcttc ccgcgcgcaa
     1741 ggtgaccgaa gagatcgcga agatccgcct cgtggaaatg ggaaaagacg tgctttctcc
     1801 tcccgggcat accgcgttcc ggacgccggt ggaaatgatg catttcatca aaaagctgcg
     1861 cgatctttcc ggcggaaaac ccgttggctt caaattgtgc gtgggcaaca agggacaatt
     1921 tctcgccctg tgcaaagcga tggtgaagac gggcatcatg ccggatttca tcaccgtcga
     1981 tgggggagaa ggaggcacag gtgcggcgcc gctcgaattt tccaatcacg tcggcatgcc
     2041 gctgcgcgat gccgtggcat tcgtatacga tgcgttgaac ggttttgcgc tgaagcgcca
     2101 catccgcatc attgcatcag gaaaagtttc ttccggtttc gagatcgtga aaaaccttgc
     2161 gctgggcgcg gacatgtgca acagcgcgcg tgcgatgatg cttgcgctcg gctgcataca
     2221 ggcgctggaa tgcaacacga atacatgtcc cacgggcgtg gccacgcagg acaaatcgct
     2281 gatgaaagga ctggtggttg acgataagaa ggtccgcgtg atgaattttc acaatgaaac
     2341 ggtgaaaagc gccgtcgaac tgatggcggc cgccggcatc gacaagcccg gcaagctgca
     2401 ccgctcgcac atttaccgcc gcatcagtcc cggcgaaata cggacgtacg ccgaaaccta
     2461 tccttacatt cttcgcggct gcctgcttga gccgcctttt ccggcgcagt atgagctcga
     2521 catggccaat tgccacgagg aaacgttcga atcggcgata caatacgcct gacgcaatcg
     2581 ccgatgaaca catccctgct ggaagatgaa ttccgctcga aggtgagcat cccggacggt
     2641 gaatttgaaa agttcagcgc gttgctcgag tctttttcca ttcgccggaa cgaacatttt
     2701 ttccaggccg gcgacgtagt gaaattcgcc gtttttatcc tgaaaggctg tcttcgccag
     2761 tattacatca gcccggaagg cgtggaaagc acggtgtatt tcgccgaaga aaaatggtgg
     2821 tcgggagatg catcgagttt ccggaacgct acgccgacaa acctgaatct ccaggcgatg
     2881 gaagattgcg aggtgctgct gctttcgagg aaaaactggc agtacgcgtt cgatacgttc
     2941 gcgtggtaca gggaatatca cacgaaagga cagcaacgcc tcgccgcgcg catgcaggaa
     3001 aagctcgggc aggcgctcac cgaaaccgcc gagcagaaat acctccgcct gctgaaggaa
     3061 aggccctcgc tgatccggcg cgttccgcag cattacatcg ccacgtatct cggccttacc
     3121 ccggagacgc tgagcagggt
//
LOCUS       DASTSF010000082         3384 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_392839,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000082 DASTSF010000000
VERSION     DASTSF010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3384
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_392839"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1841
                     /locus_tag="VFU15_02345"
     CDS             <1..1841
                     /locus_tag="VFU15_02345"
                     /inference="COORDINATES: protein motif:HMM:NF036516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716638.1"
                     /translation="SCFLPQDAHSQETDNPWVYHISVDLQSVAPDKDRVKVTIMTPPV
                     NCTKIEYILPEYVPGIPTPVNAGRFVHQFYALDDKGFPLKVTKKSDNVIVLKMRKGAV
                     LKKIEYWVDDTWDMEKTNPRMSDEKFNYVPQPAGTNFQSGSSFVLNHAFLFGYLSGYS
                     SIPYYVSVIHPDDLSASGDWDFHYESPTRESYSANSYAEFVERPVMFSVSDTCGFRSG
                     NIYVHISVFSENEKVTARLVRRLIAAQITSETNFIPDAPPSDYYLLFYFTTPFKTVLN
                     VHGGYGGLAHAHNAFYFLAEQVDEDMLASDLQSQISGDLLHLMRPVDLMTTCGNADFL
                     KPQVSPEWWFSQGTAMYFSWLSAVRDSFVSEEEFMSAVSAKVKLSGLFPLTPIAETKS
                     IVKQSGEPLKREALKARAMLLAMLLDITLTESTGGKTGLREAVMQMNAMNRFYPDSLN
                     FYLERIGGVAVKNFIADYVTGEKSPDLIRSFNKIGWAYAPETIDSVLTFGRFGLLYDE
                     EKDVFFVHNADTTNAFGLRDGDRVVSINGVIVSAGNFDETMGPVYSPKKDEAVDIIFI
                     RGSMNLEITAYPKKTVILVEHLIRMDPAAGSDALLLHQRIFTAVYN"
     gene            1844..2194
                     /locus_tag="VFU15_02350"
     CDS             1844..2194
                     /locus_tag="VFU15_02350"
                     /inference="COORDINATES: protein motif:HMM:NF025178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEU4716639.1"
                     /translation="MFRTIIRWIRIADSEDEFPLGKITVRYAAGRKLCLAKTQEGLFA
                     IEEKCPHNGFSLAMGWCTEDGKAIVCPLHRYVFEFSTGRARSGTGAARVFPVEVRGDG
                     VFLGVEEHEWGLGF"
     gene            2259..3077
                     /gene="panB"
                     /locus_tag="VFU15_02355"
     CDS             2259..3077
                     /gene="panB"
                     /locus_tag="VFU15_02355"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008468582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEU4716640.1"
                     /translation="MSVHRKEVRKVTTHVLQEMKNSGEKIAMLTAYDYTTARIIDAAG
                     IDVILVGDSASNVMAGNVTTLPVTLDQMIYHASSVVRGVDRALIVVDLPFGSYQGNSK
                     EALNSSIRIMKESGAHAVKLEGGREVKESIERILTAGIPVMGHLGLTPQSIYKFGTYT
                     VRAKEEEEAARLISDAHVLEDAGCFALVLEKIPATLAEKVAKEISIPVIGIGAGGGVD
                     GQVLVFHDMVGLTNEFHPRFLRRYLNLYEEITGAVGKYISDIKAKDFPNPNEQY"
     gene            complement(3114..>3384)
                     /locus_tag="VFU15_02360"
     CDS             complement(3114..>3384)
                     /locus_tag="VFU15_02360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716641.1"
                     /translation="SLDAGHNRHLEIKRIQTQPGHLPSVRRIFLTIDNDSRLVESHIN
                     GKSGYYMFDQVTTYFYSEKNVPVRTVTEDKLRRQKSWTKYEYEYY"
BASE COUNT          807 a          858 c          937 g          782 t
ORIGIN      
        1 tatcctgctt tctgccgcag gacgcacatt cccaggaaac cgacaatccg tgggtctacc
       61 atatttcagt cgacctgcag agcgtggcgc ctgacaaaga ccgggtaaaa gtgacgatca
      121 tgacgccgcc ggtcaattgc acgaagatcg aatacattct tcccgaatac gttcccggca
      181 tccccacgcc cgtcaatgcg ggccgtttcg ttcaccagtt ctacgcgctc gacgataagg
      241 gtttcccgct gaaggtcacg aagaaaagcg acaacgtcat cgtgctgaaa atgcgcaaag
      301 gagccgtgct gaaaaagatc gaatactggg tggacgatac gtgggacatg gaaaagacga
      361 acccgcggat gagcgatgaa aaattcaatt atgttcccca gccggccgga acgaatttcc
      421 agagcggaag cagcttcgtc ctcaatcacg ccttcctgtt cggctatctt tccggctatt
      481 cgtccattcc ctattatgtc agcgtgatcc acccggatga tctttcggcg agcggcgact
      541 gggattttca ttatgaaagc ccgaccaggg aaagctattc ggcgaattcg tatgccgagt
      601 tcgtggaacg gcccgtgatg ttttcggtga gcgacacctg cggttttcgt tcggggaaca
      661 tttacgttca tatttctgtt ttttcagaga acgaaaaggt cacggcaaga cttgtgcggc
      721 ggctgatcgc tgcccagatc acttcagaaa cgaattttat tcccgatgcg cccccgtcgg
      781 attattacct gttgttttat tttacaacac cgttcaaaac ggtgctgaat gtgcacggag
      841 gttacggcgg acttgcgcac gcgcacaatg cattttattt cctggccgag caggtggacg
      901 aagatatgct ggcttcggat ctgcagtcgc agatatccgg cgatctcctg caccttatgc
      961 ggcccgtcga cctgatgacc acgtgcggca acgctgattt cctgaaaccg caggtcagcc
     1021 cggaatggtg gttcagccag ggaacggcga tgtatttcag ctggctttcc gccgttcgcg
     1081 actcgttcgt cagcgaggag gaattcatga gcgcggtgag cgcgaaagtg aagctgtcgg
     1141 gacttttccc attgacgccg atcgcggaaa caaaatcaat tgtgaagcaa tcgggagaac
     1201 cgctgaaacg ggaagcgctg aaggcaagag cgatgctgct ggccatgctg ctcgatatca
     1261 cgctgaccga atcgaccggc gggaaaacag ggctgcggga agccgttatg cagatgaatg
     1321 cgatgaaccg cttttacccg gattcgctga atttttacct cgagcggatc ggtggcgttg
     1381 ccgtgaaaaa ttttatcgcg gattatgtca ccggtgaaaa atctcccgac ctgatccgtt
     1441 cgttcaacaa gatcggctgg gcttacgcac ccgaaaccat cgattccgtg ctcacgttcg
     1501 gccgcttcgg tttgttgtat gacgaagaga aagatgtttt tttcgtgcac aatgccgaca
     1561 cgacgaatgc tttcggtctc cgtgacggcg accgcgtggt cagcatcaac ggggtaatcg
     1621 tcagcgcagg aaatttcgat gaaacgatgg gacccgtcta ttccccgaaa aaagatgaag
     1681 ccgtggacat cattttcatc cgcggaagca tgaacctgga gattaccgct tacccgaaga
     1741 aaaccgttat cctcgtcgag catctcatcc gcatggatcc tgcggccggc agtgatgcac
     1801 ttctcctgca tcagcgtatt tttacagcgg tttacaatta aaaatgtttc gcaccatcat
     1861 acgctggatc aggatcgccg attctgaaga tgaatttccg ttgggaaaga tcacggtgcg
     1921 ttacgccgcg gggagaaaat tgtgcctcgc aaaaacgcag gaaggattgt ttgcgatcga
     1981 agaaaaatgc ccgcacaacg gcttttcctt ggcgatgggc tggtgtaccg aggacgggaa
     2041 agcgattgtc tgcccgctgc accgctatgt tttcgaattc agcacgggga gggcgagaag
     2101 tggaaccggc gccgcacggg tattcccggt ggaagtgcgc ggggacggcg tttttctcgg
     2161 tgtggaagag cacgaatggg ggctcggatt ttgaatcgtc ggaaatattt gcggtaactt
     2221 tgtcctgtaa ttaaaccggt ttttacctca gccacgccat gtcagtacac agaaaagaag
     2281 tcaggaaagt cacgacgcac gttctgcagg agatgaaaaa ctccggcgag aagatcgcca
     2341 tgctgaccgc gtacgattat acgaccgcac ggatcatcga tgcggcagga atagacgtca
     2401 ttctcgtcgg cgattccgcg tcgaacgtga tggcgggtaa tgtcaccacg cttcccgtta
     2461 cgctcgacca gatgatctat cacgcctcga gcgtggtgcg tggcgtcgac cgtgcgctga
     2521 tcgtcgtgga tcttccgttc ggttcctacc agggaaattc caaggaagcg ctcaactcgt
     2581 cgatccgcat catgaaggaa tccggcgcac acgcggtgaa gctggaaggc ggaagagaag
     2641 tgaaggaatc catcgaacgc atcctgaccg cgggaattcc tgtcatgggt catctcgggc
     2701 tcacaccgca atcgatctat aaattcggaa cgtatacggt ccgcgcgaaa gaagaagagg
     2761 aagctgcgcg acttatttcc gatgcgcatg tgctggaaga tgccggttgc tttgcgcttg
     2821 ttcttgaaaa aattcccgcc acgctcgcgg aaaaagtagc aaaagaaatt tccattcccg
     2881 tgatcggcat cggcgcgggc ggaggagtgg acggccaggt gctcgtgttc cacgatatgg
     2941 tgggactgac gaacgaattc catccgcgtt ttttacggcg ttacctcaat ctttacgaag
     3001 agatcacggg agccgtggga aaatacatca gcgatatcaa agcgaaagat ttccccaacc
     3061 cgaacgagca gtattaaccg ggatcccggc aatcgccgtt ttttcgatgg cgttcaataa
     3121 tattcgtact cgtatttcgt ccacgatttc tggcggcgaa gcttgtcttc cgtaactgta
     3181 cgtacgggaa cattcttttc ggaatagaaa tacgtcgtca cctggtcgaa catgtaataa
     3241 ccgcttttcc cgttgatatg cgattcaaca agacgggaat cattgtcgat cgtaagaaag
     3301 atgcgccgga cggaaggaag atgcccgggc tgcgtctgta ttcttttgat ctcgagatgg
     3361 cggttgtgtc ctgcatctag gctg
//
LOCUS       DASTSF010000083         4037 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_839521,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000083 DASTSF010000000
VERSION     DASTSF010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4037
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_839521"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="VFU15_02365"
     CDS             <1..324
                     /locus_tag="VFU15_02365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU4716642.1"
                     /translation="IYQQTEKFATFSETIIGSVKGMLNEPVIPFLNHIHQPCLVIFGE
                     NDQLIPNRFVHPLMNVHVIGDIARQKIRKAAVHYFSQCGHYVPFEKPHEFSDELRQFF
                     LSTED"
     gene            complement(450..1919)
                     /locus_tag="VFU15_02370"
     CDS             complement(450..1919)
                     /locus_tag="VFU15_02370"
                     /inference="COORDINATES: protein motif:HMM:NF016906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEU4716643.1"
                     /translation="MKNHAKKTLLAILPAIVILFSASAQTNVSGGIFANTTWSAANSP
                     YIITSSVVVYPGIVLTIQPGVTVKFADGQVLEIRQAQLISTGTSVAPITFTSNSGSPA
                     AGIYSGIYLNGVNVSHTFDYCNFTYADYAIRTNSSSSLSITNSSFISNNTGVSAGPAT
                     SVTIDNSLFRFNANNGIYITGGSVSNSRIVQNGTGLDLLGNPATVTNCVVDSNSTGVN
                     NAYATFSHCSFSHNVMGLYYGGMTLDSCTVNYNQTGIGTLIGSTVKNSVIDFNTSVGV
                     NIIWSGATIRNCEIKNNATGIADYREGNLSYPHNTITNNIIENDSIGILLAMHGENVY
                     CNKICNNTAYGIKYTDPQNTSVANNYWCTPDSASTEAVIYDAHDNIGSGILTIMPLDT
                     VQCYLNNPTATGIINYGAGNFSFNIFPNPATTNLTITLPDTVSNAEIRIFNMVGELEY
                     ASTTIKSITQIDVTNFESGMHIVQLTMGNSVARQKFIKQ"
     gene            complement(2149..2230)
                     /locus_tag="VFU15_02375"
     tRNA            complement(2149..2230)
                     /locus_tag="VFU15_02375"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2194..2196),aa:Leu,seq:tag)
     gene            2336..3232
                     /locus_tag="VFU15_02380"
     CDS             2336..3232
                     /locus_tag="VFU15_02380"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014678736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEU4716644.1"
                     /translation="MISLDPKEHKTSVVHQYLLHAVAPRPIAFASTVDKDGHPNLSPF
                     SFFNVFSANPPVVVFSPSRSGRTGTTKNTFDNVKEVGEVVINVVNYALVQQASLSSTE
                     YAKGVNEFVKAGLTEIPSVKIRPPRVKESPVQMECVVKDIIELGDKGGAGNLVICEVV
                     MMHINETIMNAEGTMIDQQKIDLVSRLGGDWYCRASGDALFRVAKPLLTIGIGVDQLP
                     EHIRTSPVLTGNHLGQLGNVEKIPTPEDVQKFKVSGAILEVFQDYGKDHGKLDLHLHH
                     IAARLLDEGKVEEAWKVLLSDL"
     gene            3629..4012
                     /locus_tag="VFU15_02385"
     CDS             3629..4012
                     /locus_tag="VFU15_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017733078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3127 domain-containing protein"
                     /protein_id="HEU4716645.1"
                     /translation="MFNITGTLKVKFAEQQVSERFRKRDFVITDNSSQYPQVISFQLV
                     QDRCSLIDPFNAGDEIRVFFNLRGREWKSPQGDTRYFNTLEAWKIEGVSNSQNQGNPG
                     AMNTPMTAPDVTSFSGGEGGDDLPF"
BASE COUNT          986 a          956 c         1025 g         1070 t
ORIGIN      
        1 atctaccagc agacggaaaa attcgcgacg ttcagcgaga cgatcatcgg ttcggtaaaa
       61 ggaatgctga acgagccggt gatcccgttc ctcaatcaca ttcaccagcc ctgtctcgtg
      121 atcttcgggg aaaacgacca gcttattccc aaccgttttg tgcacccgct gatgaacgtt
      181 cacgtgatcg gcgatatcgc gcggcagaag atccgcaagg cggccgtgca ttatttcagc
      241 cagtgcgggc attatgtccc gtttgaaaag ccgcatgaat tctcggacga gctgcggcag
      301 ttcttccttt cgacggaaga ttgaacagtt cccggtaaga tatcctgttc tggtctgcgg
      361 ggccgggtgc aaccccggtg aaaatttcag cttatggaag cttcagcatt ggcgcgggga
      421 ctcgaacccg caatgccggt ttttgctttt tattgcttga tgaatttctg tctcgccacg
      481 ctgttgccca tcgtcaattg aacgatgtgc attccgcttt caaaattcgt tacgtcgatt
      541 tgcgtaatgc tttttatcgt ggtggaagca tattccagct cgccgaccat gttgaatatt
      601 ctgatttctg catttgaaac agtatcgggt aacgtgatgg tcaaattagt ggtagcaggg
      661 ttcgggaaaa tgttgaacga gaaatttcct gccccataat tgatgatccc cgttgccgta
      721 ggattattca aataacattg gacggtatca agcggcatta tggtcaggat gcccgagcct
      781 atgttgtcat gcgcatcata aatgacagct tcggttgatg cggaatcagg agtacaccag
      841 taattgttgg cgacagaagt attctgcggg tcggtatatt ttatgccgta agccgtgttg
      901 ttgcatattt tgttgcaata aacgttttcc ccgtgcatcg caagcaggat gccgatgctg
      961 tcattctcaa tgatattgtt tgtaatggtg ttgtgcggat aagagagatt cccctccctg
     1021 taatcagcaa tgccggttgc gttgtttttt atttcacaat tgcggatggt agcgccgctc
     1081 cagataatat tcactcctac gctcgtgttg aaatcaatca ctgaattttt tacggtgctg
     1141 cctatcaagg tacctatccc ggtttgattg taattaacgg tgcagctgtc aagagtcatc
     1201 cctccataat agaggcccat tacattatgg ctgaatgaac aatgtgaaaa tgttgcgtag
     1261 gcattgttga cacctgtaga attagaatca acaacgcagt ttgtcaccgt ggccgggtta
     1321 cccagcagat ccaatcctgt tccattctgc acaatgcggg aattggaaac acttccgcct
     1381 gtaatgtaaa ttccgttatt cgcattaaac ctgaaaagag aattgtcgat tgttactgaa
     1441 gttgccgggc ccgcggaaac tcctgtgtta tttgaaatga aactggaatt tgtaatggac
     1501 aggctggaag aggaattggt ccggatggca taatcggcgt acgtgaaatt gcaataatca
     1561 aatgtgtgcg aaacgtttac cccattcaag taaatgccgc tgtatattcc cgctgccgga
     1621 gagccggaat tgctggtgaa cgtaatggga gcaacgcttg tgcctgtcga aataagttgc
     1681 gcctgcctga tttcaagcac ctgcccatcc gcaaatttta cggttactcc cggctgaatg
     1741 gtgagtacaa ttccgggata caccacaaca gaggaagtga tgatatacgg actgtttgcc
     1801 gcggaccagg ttgtgtttgc aaaaattccc ccgctcacat ttgtctgtgc gcttgcagaa
     1861 aataaaatca ctattgccgg gagaatggcc agtagtgttt tttttgcgtg gtttttcatt
     1921 ttgtgttgcg ttttgaagcg gaggtcattc atgcgagcaa acgttcaaag aggtgattta
     1981 ttacatagta aggtataata atactcaatg ttcgggcatg cccctttata gaaacacgtg
     2041 tgaatttgac cggaggttgg gtatttgaaa aaataaagag gaaatgggtt tagccgggct
     2101 ttcaatgaaa aaagaccagc cgtttcaggt tgatcttttt tcgctctgtg cgggcggagg
     2161 gactcgaacc cccatgcctt gcggcgccag atcctaagtc tggtatgtct accagtttca
     2221 ccacgcccgc atgcgaggtg acgaaattag ctatttcggc tgacccgttt tcattcctgt
     2281 tgccggccgg ccgatttttt acccggaaag ctggtcggca aactatcttt gcagcatgat
     2341 ttcactcgat cccaaagaac acaaaacatc cgttgttcac cagtacctgc tgcatgctgt
     2401 tgcgccccgt cccattgcat ttgccagcac cgtcgacaag gacgggcatc cgaatctcag
     2461 cccgttcagc ttcttcaatg tcttttcggc gaatccgccg gtcgtggttt tttcaccttc
     2521 acgcagcggg agaacgggca cgacgaaaaa tacattcgac aatgtgaagg aagtcggtga
     2581 ggtggtcatc aatgtcgtca attatgcgct cgtacagcag gcatcgcttt cgagcacgga
     2641 atacgccaaa ggcgtgaatg aattcgtgaa agccgggctg acggaaattc cgtccgtgaa
     2701 gatcaggccg ccccgcgtga aagaatcgcc ggtgcagatg gaatgcgtgg tgaaggatat
     2761 catcgagctc ggtgataaag gcggagccgg caacctcgtg atctgcgagg ttgtgatgat
     2821 gcacatcaat gaaacgatca tgaacgcgga gggcacgatg atcgaccagc agaagatcga
     2881 tcttgtttcg cgtttgggcg gcgactggta ttgccgcgct tcgggcgatg cgcttttccg
     2941 cgtggcgaaa ccgctgctga ccatcggtat cggcgtcgac cagcttccgg agcacatccg
     3001 cacgagcccg gtgcttaccg ggaatcacct cggtcagctg ggcaacgtgg aaaaaattcc
     3061 tacgccggaa gacgtgcaga aattcaaagt ttccggcgcc attctcgaag ttttccagga
     3121 ttacggaaaa gatcacggga agctcgacct ccacctgcat cacattgcag cgcgcctgct
     3181 ggatgaagga aaagtggaag aagcctggaa agttttactg agcgatctct gatccggcct
     3241 tcctctgcgg atcacggatt ttccggtcgc tgttttcgtg gggcggggac agtaaggaga
     3301 acattgcttc cctgggattt tacctgcatg tttttcctgc ctggaaagct gtaaatgctt
     3361 tcaagtggtt tcagtcgttt aatcccgttt gcaggcgttt ggatgcgcaa acagcctttt
     3421 tgcagtgggt tgaatatcat tggcaaagga tttttgaaca aacttgcaaa tgcggaaaaa
     3481 ttgtcgcact aaccttgcag gttataacct gcaaggttgt caaggtgagg gcaggcagga
     3541 gcaacacgct tttatcaaca agctttccac attccatttt caccttcact attgtcctac
     3601 ttttacagtt aattaaaaaa tcaagctcat gttcaatatc acaggtactc ttaaagtaaa
     3661 attcgctgaa cagcaggttt ccgaacgttt ccgcaaacgc gattttgtca tcacagataa
     3721 ttcttcccaa tacccgcagg tgatcagctt ccagctcgtc caggaccgct gttccctcat
     3781 cgatccgttc aatgcgggcg atgagatccg cgtattcttc aatcttcgcg gacgtgaatg
     3841 gaaaagcccc cagggcgata cccgttattt caacacgctc gaggcctgga agatcgaagg
     3901 cgtaagcaac agccagaacc agggcaatcc cggtgcgatg aacacaccga tgacagcccc
     3961 tgacgttact tcattttccg gcggagaagg cggcgacgac cttccgttct gacggtttca
     4021 tattctcact gatgaag
//
LOCUS       DASTSF010000084         7748 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1510409,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000084 DASTSF010000000
VERSION     DASTSF010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 7748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7748
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1510409"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1023
                     /locus_tag="VFU15_02390"
     CDS             <1..1023
                     /locus_tag="VFU15_02390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716646.1"
                     /translation="ENLLKEREGYVKLDLLKCHSGKEWDDAVDKALKDGAGVVLVDHF
                     EYDSFNSRTNRIKLVLLEKLACLPEKRVIIISSVPIQSFTGMNVALPAEEELEKEVNA
                     HLQTRDRWSKTLGSFYDFWFPIKGVNNEGAPSMSRLVRSKNVPEEIRDVIVEECEHGF
                     FLRKIGEDLCESMKDETLKRTDPRSVRFAREGIVLKVQQLAHSYYLAIWSLLTPEEQY
                     VLFDIAQDGLVNLKNLEVVDLLVKKGLIVSGSSLQVMNRSFQNFILTVVEPADLMKIE
                     YSTRESGAWNRFRKPLIIFVIALILFILKSDQNETFTWITGFAAGVPLVINLLAALMK
                     GEIGKE"
     gene            complement(1193..1753)
                     /locus_tag="VFU15_02395"
     CDS             complement(1193..1753)
                     /locus_tag="VFU15_02395"
                     /inference="COORDINATES: protein motif:HMM:NF024934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEU4716647.1"
                     /translation="MHGRRFGNAPVLRPGRKRKVKTFRANESGKEYITGLYLENDFFG
                     YLALITGNVRNESAMALEDSEVALLPKQEFQQQLASDTTLSFLFISFVMNSLNEAEQR
                     LLKLAYNSARSRVVEALLFLFRKYKAREGDTIPLSRENISTLAGISPESVSRNLGELR
                     NEGLIEILHGAVKLCNLYKLEHLRAC"
     gene            complement(1693..2256)
                     /locus_tag="VFU15_02400"
     CDS             complement(1693..2256)
                     /locus_tag="VFU15_02400"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU4716648.1"
                     /translation="MKDKVLLIENCQEMLDNAAELLHLAGYSVQTAGNGIEGLMRLKT
                     ERPEIILCDIVMPGLDGYGVLRAVRNMPELAGVPFIFMTAKAEKKDFRKGMDLGADDY
                     LIKPFSGEDLLRVVEARLEKSRQAANKNGSAGKNITELVRETKNFLQLAMFSRKRETK
                     KFRKKDMIFMEGDSGTHLFFVLAGKEK"
     gene            complement(2566..3060)
                     /locus_tag="VFU15_02405"
     CDS             complement(2566..3060)
                     /locus_tag="VFU15_02405"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEU4716649.1"
                     /translation="MKVGEIMIIQDLQYCTPETFLRDAAAIMKTAGVSALPVVDAERQ
                     VIGIVTDRDIDKLPVQKQAPERIRDIVAHKAVTVSAGDELTFALRQMRAVRIARLPVV
                     DGEGRLEGILCIHHLLAESAGNRKVLGGLSLIAGEILKTVTMLSNKYTGHNSLARMQK
                     KRKE"
     gene            3871..4854
                     /locus_tag="VFU15_02410"
     CDS             3871..4854
                     /locus_tag="VFU15_02410"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF006426.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoglucose/phosphomannose
                     isomerase"
                     /protein_id="HEU4716650.1"
                     /translation="MKNLISNFAAQLATATEIAGHTVLTASPEEIRNVVICGMGGSGI
                     GGNIAAELAAPGSPVPITVVKDYFLPGFVNENTLFIASSYSGDTEETIHALEEATRKN
                     AHIVCITSGGQVRAIAERSGLDLFLIPSGLPPRAALGYSLTQLLCALEFHRIIPGSYQ
                     KQINGAILLLSQEENNIIRSAKESALVLKDRIAVIYGTAGMESVALRFRQQINENSKA
                     LCWHHVFPELNHNEIQGWRIENEQLAVVIFRNGNDYIRTAKRIDVTAGILEHYSTPII
                     EIFSKGDSPLARTLYLIHWGDWVSYFIAEFKGLDTMDISVIRYLKSELAKL"
     gene            4859..6850
                     /locus_tag="VFU15_02415"
     CDS             4859..6850
                     /locus_tag="VFU15_02415"
                     /inference="COORDINATES: protein motif:HMM:NF017964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HEU4716651.1"
                     /translation="MIRPEFDQQQLHHLRFSKNREILMVNKNGSYCSTSVSTCNTRKY
                     HGLLVSPQPKIDSDNHVLLSCLDDTVIYDEKEFRLSVHQSPGVHQPEGYLLMESFTWE
                     GYARWTYNADGIIIAKELILDQNEDRVFIRYFFSRPGAILRVAPFIACRNIHTLRAGN
                     HRVNVNTENGKNGLAVGMPGEYAPLHFQVSLPSSFIRSPDWYDNIEYEQEQLRGYEFR
                     ESLFVPGYLEVETGDAQEVVFCAGIKPMTASRISRRFFAEKKSRKMTEGLDQCLDTAA
                     SLFIVKKKQGTEIVAGWPWFGHWGRDTFIALPGLTLTVRRAKIFESVMQDMLRDFRDG
                     LFPNTGRGKNASYNAADTSLWFIRALQQYTEYTRKPELTWKYYHEAVISVLENYRSGM
                     LPHIAMQENGLLRAEKAGKALTWMDAIVDGHPVTQRNGLAVELNALWYNAICFALYLA
                     GKAGNAAFINDWKHIPRAIEKSFPETFWSEEKGYLADCVNDSGTDWSLRPNQVIAVAL
                     PFCPVGKEISEAVLAVAEKKLLTPRGLRTLSPGDPCYKGIYAGNPRERDLAYHQGTVW
                     PWLLGYYAEACLRVRGKAALEQINILYHGFEDALYEYGIETIPEIYDGDEPHRPKGCI
                     AQAWSTGELLRVRQMLRKNGYKPGAEKTRSVVAHVKAEL"
     gene            6847..>7748
                     /locus_tag="VFU15_02420"
     CDS             6847..>7748
                     /locus_tag="VFU15_02420"
                     /inference="COORDINATES: protein motif:HMM:NF019924.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU4716652.1"
                     /translation="MKVLMFGWEFPPHITGGLGTACAGIVRGLLDQDVTPLFVVPRLH
                     GGEDRATFRLVNASEIPVPRQAQREWLHKLTYIEADADLVPYDSPAEFSGAAAVPGAL
                     EEKGNLSSPAGHVQYRFSGGYNRDLLKEVYRYALIAAEIAGTHPHEIIHAHDWLTFPA
                     GIAARSVTGKPFIAHVHATEFDRSGENINRQVYEIEKQGMEEADHIIAVSGFTRQLII
                     TRYGIPPGKVTVVHNGVSKNTFPAPAVFHKPAKKIVTFAGRITMQKGPEYFIEAAAKV
                     LEKYPEVHFVMAGSGDLMQGMIRKA"
BASE COUNT         1922 a         2040 c         1975 g         1811 t
ORIGIN      
        1 gaaaacctgc tgaaggaacg cgaaggctac gtgaagctcg atctcctcaa atgccacagc
       61 gggaaagaat gggacgacgc ggtggacaaa gcgctgaaag acggagcggg cgtcgttctt
      121 gtcgatcact tcgaatacga cagcttcaat tccagaacga accgcatcaa gctcgtgctg
      181 ctcgaaaagc tggcgtgcct gccggagaaa cgcgtgatca tcatttcttc cgttcccatc
      241 cagagcttca ccgggatgaa cgtcgcgctt cccgcagaag aagagctgga aaaagaagtc
      301 aacgcgcacc tgcagaccag ggaccgctgg agcaaaacgc tcggttcctt ttacgatttc
      361 tggttcccga taaaaggcgt gaacaacgaa ggcgctcctt ccatgagcag gctcgtgcgt
      421 tcgaagaatg tccctgaaga gatccgggac gtgatcgtgg aagaatgcga gcatggattt
      481 ttcctgcgca agatcggaga agatctctgc gaatcgatga aggacgaaac gctcaagcgg
      541 accgatccgc gttccgtgcg tttcgcgcgc gaaggcatcg tgctgaaagt gcagcagctc
      601 gcgcattcct attacctcgc gatctggtcg ctgctgacac cggaagaaca atacgtgctc
      661 ttcgacattg cgcaggacgg gctggtgaac ctgaaaaacc tggaggtcgt cgaccttctg
      721 gtgaagaaag gactgatcgt gtccggctca tcgctgcagg tgatgaaccg cagcttccag
      781 aatttcatcc tcaccgtcgt cgaaccggcc gacctgatga agatcgaata ttcgacgcgt
      841 gaatccggcg cctggaaccg cttccgcaag ccgctgatca ttttcgtcat cgcgctgatc
      901 ctgttcatcc tcaaatccga ccagaacgaa accttcacgt ggatcacggg gttcgctgcc
      961 ggcgttccgc tcgtgatcaa cctgctcgcc gcgctgatga agggagagat cgggaaagaa
     1021 tgatccgcat gataaatcat gctggaccct ggaacctgcc cggatttctg ccttcgggtt
     1081 tgttatgcgt cattcttcag tgacagttcg ctgttctttt tgaacgtaac gaatccgcta
     1141 tgtcccgcaa aaaccgcttt ttatccggcc ggcaaaagca aaacaaaatt ccctaacagg
     1201 ccctgaggtg ctcaagctta tacaggttgc aaagcttcac tgcaccgtga agtatttcga
     1261 taagcccctc attgcgcagt tcgcccaggt tgcggctgac ggattcaggt gaaattccgg
     1321 ccagtgtcga aatattttcc ctgctcagcg ggatcgtgtc tccttccctg gccttgtatt
     1381 tcctgaacag gaagagcaaa gcttccacta cccggcttct cgcagaatta taagcgagct
     1441 tcagcaggcg ctgctccgct tcgttaagac tgttcataac aaaggagatg aacaggaacg
     1501 aaagcgtagt gtccgaagcg agctgctgct ggaattcctg cttcggcaac agcgccactt
     1561 ccgaatcttc caaagccatc gccgattcat tccgtacgtt gccggtgatc agagccaggt
     1621 aaccgaagaa atcgttttca agataaagcc cggtgatgta ttctttaccc gattcgttcg
     1681 cgcggaatgt ttttactttt cttttccggc caggacgaag aacaggtgcg ttcccgaatc
     1741 tccttccatg aatatcatgt ctttcttcct gaactttttc gtttcccgtt tccgggagaa
     1801 cattgcgagc tggagaaaat tctttgtctc ccggaccagc tccgtgatgt tctttcctgc
     1861 ggacccgttt ttgttagcgg cctgcctgct tttttccagc cgtgcctcca ccacgcgaag
     1921 caaatcttcg ccggaaaaag gcttgatcag gtaatcgtcg gcaccgagat ccatcccctt
     1981 cctgaaatcc tttttttccg ctttggctgt catgaaaata aagggcacgc cggctaactc
     2041 cggcatgttc cgcacagcgc gcagaacgcc atacccgtcg agtccgggca taacgatatc
     2101 gcaaagaata atttcgggac gttcggtttt caggcgcatc aaaccttcga tgccattgcc
     2161 tgctgtttgc actgaataac ctgcgaggtg aagaagttct gctgcgttgt ccagcatttc
     2221 ctggcagttt tcgataagaa gtactttgtc tttcatggaa gaagtgtttt tgatgtcaga
     2281 ttcaaaactc atcttcctgg cgctaattat gggggagaat taaaaccgag gagatttcag
     2341 catgtatgta aaagagcgga aaaagacaga taacttatat taaaagtaat ttatttccag
     2401 ggataagcta accaggtatc attaaattaa ataaaacccg gagcacggat aaccggcgaa
     2461 agggaacctg tcaaagcagc ccgtgatttt gccgcttccc gaaaatgaac cacgaagtcc
     2521 gccggcattc gttggtaacg atctttccat ctgtcatttt ttcgtttact cctttctttt
     2581 tttttgcatg cgggccagtg aattatggcc ggtgtatttg ttgctgagca ttgtaacggt
     2641 tttcaggatt tctcccgcga tgagtgaaag accgccgagc actttcctgt ttccggcgga
     2701 ttctgcaagc aggtggtgaa tgcacaggat gccttccagc ctgccctcac cgtccaccac
     2761 cggcaaacgc gcaatcctca ccgcacgcat ctgccgcaac gcgaacgtca gctcatcgcc
     2821 ggcggaaacg gtcaccgcct tatgcgccac aatatcccgg attctctccg gcgcctgttt
     2881 ctgcacaggc aacttgtcaa tgtcgcggtc cgttacaatt ccgatcacct gtctttctgc
     2941 atctactacc ggcaatgcac tgacgccggc cgtcttcatt attgccgccg catcgcgcag
     3001 gaatgtttcc ggtgtgcaat actgcaaatc ctggatgatc atgatttccc cgactttcat
     3061 tacgatcgtt tttttgcggt tgtcctgcaa aagaaaacac atgggcattt aaaagaaatt
     3121 aaaactgtca acgtctccgg ccggagacat cactctgtac cttgacgcat gtcattcata
     3181 catacgccgg taaaagccat tccctgctgc ttctcatgca cgggtttcaa tttcccggaa
     3241 agggcatcac cggtcttaac gcaagtggtt ttgtttttca aaaagatcag cgcccgctgc
     3301 tgatcttttc tgctgtgtaa agaccctttg actcctcggg caaaagatca cgaagtgatg
     3361 agattttgtt catgggtagg tttttgacga actgtaaaac tatttcgcct gcctggcgca
     3421 agctatgact gttgtcaaaa gacgcagtat gatccgtcac atccgcgcgg atatccaccg
     3481 aagaaccgtt accgtttttc ccgtgctcct cacaaaatac caaatcggga aacgcggagt
     3541 ttttttaatc gtggattccg gcaactgaat gacggaagat aaattccatt ggtttataaa
     3601 gccggcatta ctaaatcgta aacggagatt tgataaagat caagaccggc aatgacagat
     3661 taggttggct gcggaggtgt tgcaaaatag ttttgttcag aatgtggtaa ggaatattgc
     3721 atcatttaac cggcgggcag tgatggagaa aagatgagtg tttttggggt tcagccggat
     3781 aattctgccc cggcaaattt taatacgaaa aattatatcc atgctcaccg gcatccaacc
     3841 tattgattta acgaacataa acctgtacac gtgaaaaatc tcatcagcaa ttttgcagcc
     3901 cagctcgcaa cggcaacaga gattgccggc catacggtat tgaccgcatc cccggaagaa
     3961 atccggaacg tcgttatctg cggcatgggc ggttccggca tcggcgggaa tattgcggca
     4021 gagcttgccg cgcccggttc tcctgtcccc atcacggtag taaaagatta tttccttccg
     4081 ggttttgtga atgaaaacac gctttttatc gcctcttcct attcgggtga tacggaagaa
     4141 accattcatg cgctggaaga agcaacccgg aaaaatgcgc acatcgtatg catcacttcc
     4201 ggcgggcagg tccgtgcgat cgcagaaagg tcgggcctcg atctttttct tattccatcc
     4261 gggctgccgc cgcgcgctgc gctgggctac tcgctcacgc aactgctttg cgcgctggaa
     4321 ttccatcgta ttattcccgg ttcttaccag aagcagataa acggcgcgat actcctgctg
     4381 tcgcaggaag aaaacaatat catccgcagc gcgaaagagt cggcgctggt gctgaaagac
     4441 cgtatcgccg ttatctatgg cacagcggga atggaaagcg tcgcgttgcg tttccgccag
     4501 cagatcaacg aaaattccaa agcactttgc tggcatcatg tttttcccga gctgaaccat
     4561 aacgagatcc agggatggcg catcgagaac gagcagctgg cggtggttat tttccgtaac
     4621 ggcaacgatt acatacgcac agcaaaacgc atcgacgtaa cagccggcat cctcgagcat
     4681 tacagtacgc cgatcatcga gatcttttcg aaaggcgatt ctccgctcgc gcgaacactc
     4741 tacctcattc actggggcga ctgggtgtcg tatttcatcg cggaattcaa gggactcgac
     4801 accatggaca tcagcgtgat ccggtacctg aaatcagaac tcgccaaact ttaaagaaat
     4861 gatccgaccg gaattcgatc aacagcaact ccatcatctc cgtttttcga aaaacaggga
     4921 gatactcatg gtcaataaaa acggcagcta ttgcagcact tccgtttcga cgtgcaatac
     4981 gcgaaaatat catggcctgc tggtctcgcc gcagccgaag attgacagcg acaaccatgt
     5041 ccttctctcc tgtcttgacg atacggtcat ttacgatgag aaagaattcc gtctttctgt
     5101 ccaccaatcc ccgggcgtgc accagccgga aggttatctc ctgatggaaa gttttacgtg
     5161 ggaaggttac gcgcggtgga catataatgc agacggcatc attatcgcga aagaacttat
     5221 tctcgaccaa aacgaggatc gtgttttcat ccgttatttt ttcagcagac ccggtgcgat
     5281 cctccgggtt gcaccgttca tcgcctgcag gaacatacac acgctgcgcg cgggcaacca
     5341 ccgcgtcaat gtgaatacgg aaaacgggaa aaacggcctg gccgtgggaa tgccaggaga
     5401 gtatgcaccg ctgcatttcc aggtctcatt gccttcctcc ttcatccgtt cacctgactg
     5461 gtacgataat atcgaatatg aacaggagca gcttcgcgga tatgaattcc gcgaatcgct
     5521 ttttgtgccc ggctatctcg aagtggaaac aggagatgca caggaagttg ttttctgcgc
     5581 agggataaaa cccatgacag cgtcacgtat ttcccgccgg ttttttgcgg aaaagaaaag
     5641 caggaaaatg acggaaggat tggatcaatg cctggacact gcggcttccc tgttcatcgt
     5701 gaaaaaaaaa cagggaaccg aaatagttgc ggggtggcca tggttcggac actggggacg
     5761 ggacacgttc atcgcattgc cgggattgac gctcaccgtg cgccgtgcaa agattttcga
     5821 atccgtcatg caggacatgc tcagggattt ccgcgacggg ttattcccga atacggggag
     5881 aggaaaaaat gcatcctaca atgccgcaga cacttcgctt tggtttatcc gcgccctgca
     5941 gcaatacacg gaatataccc gtaagcctga gctcacctgg aaatattacc atgaagctgt
     6001 catcagcgtt cttgaaaatt accgttcggg catgctgccg cacatcgcca tgcaggagaa
     6061 cgggttattg cgggcggaaa aagcaggaaa ggcgctgacc tggatggatg ccatcgtcga
     6121 cgggcatcct gtaacgcaac gcaacggtct cgctgtcgag ctgaatgcat tatggtataa
     6181 cgcgatctgc ttcgcgcttt acctcgccgg gaaagccggg aatgccgcct tcatcaacga
     6241 ctggaaacat attccacggg caatcgaaaa atcattcccg gaaacgttct ggagcgagga
     6301 aaaaggatat ctcgccgact gcgtcaatga ttccggcacg gactggtcgc tccggcccaa
     6361 ccaggtgatc gcggtggcgc ttccgttctg cccggtcgga aaagaaattt cagaagccgt
     6421 tctcgctgtc gcggaaaaaa aacttctcac tccccgcggt ctccgcacgc tttctcctgg
     6481 cgatccgtgt tacaaaggaa tttacgccgg caatccccgg gaacgcgatc tcgcctacca
     6541 ccagggaacc gtgtggccgt ggctgctcgg ttattatgcc gaagcctgcc tgcgtgtacg
     6601 gggaaaagca gcgctggaac aaattaacat cctgtaccat gggttcgaag acgcgcttta
     6661 cgaatacggg atcgaaacca ttccggaaat ctacgacggg gacgaacctc accgtccgaa
     6721 aggatgcatc gcgcaggcat ggagcacagg cgagctcctg cgtgtgcggc agatgttgcg
     6781 caaaaacggg tataaaccgg gagcggaaaa aacaagatcc gtggttgcac atgtaaaagc
     6841 tgaactatga aagtgctgat gttcggttgg gaatttccac cgcatattac cggcggactc
     6901 ggaacagcct gtgcaggaat cgtaagagga ttgctggacc aggatgtaac accgttgttc
     6961 gtcgtgccgc gtcttcatgg cggggaagac cgggctacgt tcaggctggt gaatgcaagc
     7021 gaaatcccgg tgccgaggca ggcgcaacgt gaatggctgc acaagctcac ctacattgaa
     7081 gcggatgcag atcttgtccc gtacgactcg cccgctgaat tctccggcgc agcagctgtg
     7141 cccggagcgc ttgaagaaaa aggaaactta tcttctcccg cgggtcatgt gcagtaccgt
     7201 ttttcaggcg ggtacaaccg tgacctgctg aaagaagttt accgctatgc gttgatcgct
     7261 gcggagatcg ccggaacgca tccgcacgaa attattcacg cccatgactg gctgacgttt
     7321 cctgccggca ttgcggcacg cagcgtaacg gggaaaccgt tcatcgcgca tgtgcacgcg
     7381 accgaattcg accgttcagg tgaaaacatc aaccggcagg tctatgaaat tgaaaagcag
     7441 ggcatggaag aagcggacca catcattgcg gtgagcgggt tcacgcgcca gctgatcatc
     7501 acacgctatg gaataccgcc cggaaaggtc accgttgtac acaatggtgt ttcaaagaac
     7561 acattccctg ctcctgccgt ttttcacaag cccgcgaaaa aaattgtcac gttcgccgga
     7621 cgtataacaa tgcagaaagg acccgaatat tttatcgaag cggccgccaa agtgctcgaa
     7681 aaatatccgg aagtacattt cgttatggcc ggcagcggcg acctgatgca gggcatgatc
     7741 cggaaagc
//
LOCUS       DASTSF010000085         8274 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1954720,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000085 DASTSF010000000
VERSION     DASTSF010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 8274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8274
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1954720"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1422)
                     /locus_tag="VFU15_02425"
     CDS             complement(<1..1422)
                     /locus_tag="VFU15_02425"
                     /inference="COORDINATES: protein motif:HMM:NF027900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5060 domain-containing protein"
                     /protein_id="HEU4716653.1"
                     /translation="MKKYLSVFAAFTASQLCIAQQSPYTPVDDPLAPITSGQYNFSGP
                     GETFSPFYYDHITFTQTSETNMTAKDRVHLQPGFHAGDYTGNGYFHAFIANPDFNVVF
                     ITPDEHLPQVGQFEKLEIGMQLPASVDQQVNDFLADPNTGLNPFDPDKISVEATFTNG
                     LNSYTVYGFYYDEFTRDVNAMAPRAPANWIAQPTLYHWRIRFAPPFTGSWYCTIGIRV
                     NDEANPSYLVNGIYFTCVPSSNQGWLKKGNDNWHLAYSGSDASFFALGQNIAWTDPIK
                     PDYHTSFNGGNNPDVPNYPLYDAGYLDILNWIADLSQSGGNMVRMSAVPWNYDVEFEH
                     LNNYKDRMNRAWELDRVFDQCEQENMKISMEFEFIGKDDANGNPYFLWSNNPYHTELS
                     GVTDKAHFFSDPDARVALKKKLRYFLSRWGYSTCLGIFALTGEINGGFGSIDDYENSP
                     SLQVSQTEWEEDLFQYSKALMNYR"
     gene            complement(1424..3196)
                     /locus_tag="VFU15_02430"
     CDS             complement(1424..3196)
                     /locus_tag="VFU15_02430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716654.1"
                     /translation="MKSPGFTLCLLCLPFFLSAQAPKWSTAGNATGNGDFIGTTNNNP
                     LVFFVNDSEAMRIKQDGDLKVRNLGGNGRTLVTSNNNGVLGSLALPGNDSLYLDGNGN
                     FSSISAASGWAYNGNNLYTVNTGYVGIGVSTPQFPLEVNGSAKFNGTLYANGLILANK
                     LQADTIKGASSISVNNNLNLTGGTLNEMYALTGDVRVQSKPGYNGNTILNAGTNGNVG
                     IGIFAPQYKLDVNGDQRVSGQMYIHRIVPLAGDSEIHFGDSSVIVGVNNTGTGNYITA
                     SPAGSVKGLLIGYGSGRARAVSSLAIGRNVATDPSAAYSIAIGSGPNNVSVSSWMTNN
                     VPNSLMIGFNSTIPTLFVGPGSGTGTLGTVGIGSTAPSSLLQVNSGIQRINIDAAPAA
                     AGGILGYVGFNAELNSNGFMHTTGGANTNTGAAISLSSDGSLWFNVLPNTGSADNQQD
                     YVHMTGGTAMRIYSNYVLIGNGGLAASSQYNNDPLNKLMVQGRILCKDVVVSNIDWQD
                     EVFDSAYTLMPLDSVGMYVKINRHLPGFRPEKEIESDGMSVSATAAAQQKQIEEMMLY
                     ILQINDRLKALEAENEQLKKNGGK"
     gene            complement(3513..4376)
                     /locus_tag="VFU15_02435"
     CDS             complement(3513..4376)
                     /locus_tag="VFU15_02435"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEU4716655.1"
                     /translation="MQPARFFSPFLLILTAAWLIIYLFIFPDPAGLFLENWPIILLAV
                     AAAIVGNMTAIGGGIIFIPVFMFIYHVNPVSALKLTFVTQAVGMTSGATGWVQRKTVP
                     LKLLKWTIPSLILGSVVSTFFIHPQTMLIKSMFGPVSFFVGMLTLVLAGRKSSGDDLP
                     PKAMLPVFLVSFIGGLITGWVAIGEGEIIAAFCMLVYGLDTNKSVGLGVVLLSVNSIL
                     LALVHALYFGGVPWDLAMFTIPGVIWGGRLGPFLAKRTSATVMKRIFGWIAMLDGLLI
                     TLQVVRGHLLR"
     gene            complement(4379..7243)
                     /gene="uvrA"
                     /locus_tag="VFU15_02440"
     CDS             complement(4379..7243)
                     /gene="uvrA"
                     /locus_tag="VFU15_02440"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007094138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEU4716656.1"
                     /translation="MSEAIETYNPKQYIVIKGAKMHNLKNVDLAIPRNRFVVITGLSG
                     SGKSSLAFDTLFAEGQRRYVESLSAYARQFLGKIEKPAVEYIKGISPAVAIEQKVNTR
                     NPRSTVGTSTEIYDYLKLLFARIGKTYSPVSGEPVKRHSVSDVVDAVMGLPGGTKVLL
                     LAPLKKKKERSLKEHLGLLLQQGFTRILYRNEILRLNEINVSSLSAKEDIFIVIDRIA
                     VNPQDEDGRSRIADSVQTAFYEGEGDCVVEIAEENPQSQSPNPKSAKGKQAAAGTQNG
                     NRLYFSNRFEADGMLFEEPNVHFFSFNNPIGACKTCEGFGSVIGIDPDLVIPNKNLSV
                     YEEAIACWKGEKMGEWKEHLVMNAYKFNFPIHKPYNELSQAQKELLWTGNKHFHGLNE
                     FFKHLEEQSYKIQYRVMLSRYRGKTTCPDCRGTRLRKDANYVKVNGHSISDIVLMQVK
                     DAVKFFTAMKLDEHDRAVAKRLLTEITNRLRFLGDVGLGYLTLNRLSSSLSGGESQRI
                     NLATSLGSSLVGSMYILDEPSIGLHPRDTERLIRVLLALRDIGNTVIVVEHDEEIMRS
                     SDVMVDMGPMAGTLGGEVVFNGSHEDLLKAESLTAKYLTHREKIPVPSKRRKWKNFIE
                     VKGARENNLKNLDVKFPLDVMTVVTGVSGSGKSTLVRRILYPAVQKHLGGYGEQTGQF
                     DALKGDFRHITSVELVDQNPIGKSSRSNPVTYVKAYDDIRALYADQQLSKMRGYKASH
                     FSFNVDGGRCEVCEGEGEVVVEMQFMADVHLQCEVCKGKRFKEETLEVQYNGKNISDV
                     LEMTIDEAILFFGDGTNPGKRIVSKLQPLQDTGLGYVHLGQSSSTLSGGEAQRIKLAS
                     FLGLSNQPPTLFIFDEPTTGLHFHDIRKLLDAFNALIAQGHSLIIIEHNMDVIKCADW
                     IIDLGPEGGDEGGNIVFEGTPEQVAKAKNSYTGKFLKEKI"
     gene            7666..8250
                     /locus_tag="VFU15_02445"
     CDS             7666..8250
                     /locus_tag="VFU15_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014201140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU4716657.1"
                     /translation="MRYDQLQDQELVDLYIAGNEPCLEILIARHKKRVFSYIMMVLRD
                     RHLSEDIFQETFIKVIQTLKRGTYNDEGKFLPWVLRIAHNLIIDHFRRQKRMPVVSGG
                     EDFDIFSVLRLRQGNIEDKLIKTQVREDLRRCIEKLPPEQREVLIMRHYLDMSFKEIA
                     DSTSVSINTALGRMRYALINLRRMMDKKHVAVPA"
BASE COUNT         1866 a         2167 c         2279 g         1962 t
ORIGIN      
        1 acgatagttc atgagcgctt tactatattg aaacagatcc tcctcccact cagtttgact
       61 tacctgcaaa gatggcgagt tttcataatc gtcgatcgat ccgaaaccac cgtttatttc
      121 gccggttaag gcgaatattc ccaggcaagt tgagtatccc cagcgggaaa gaaaataacg
      181 cagctttttt ttgagggcta cccgggcatc cggatcagaa aaaaaatgtg ccttatctgt
      241 aacgccggaa agttcggtat gataaggatt attgctccat agaaagtagg gattaccgtt
      301 cgcatcatct ttcccgataa actcaaactc catcgagatc ttcatattct cctgctcgca
      361 ctggtcgaac acccggtcga gttcccacgc ccggttcatt cgatccttat aattattcag
      421 gtgctcgaat tcaacgtcgt aattccaggg aacggcggac atgcgcacca tgttacctcc
      481 gctctggctc aggtcggcga tccagttgag gatatcgagg taacctgcgt cgtaaagcgg
      541 gtagttcggt acatcgggat tatttccccc gttgaagctc gtatggtagt ccggtttgat
      601 tgggtcagtc caggcgatat tctgccccag cgcaaaaaaa cttgcatcgc tcccgctgta
      661 ggcaaggtgc cagttgtcgt tccctttttt gagccatccc tggttcgacg aaggcacgca
      721 cgtgaaataa atcccgttca ccaggtacga aggattcgct tcgtcgttca cgcgtatgcc
      781 gatcgtgcag taccagcttc ccgtgaacgg cggggcaaag cggatgcgcc agtggtaaag
      841 tgtgggctgc gcgatccagt tcgccggcgc gcggggcgcc atggcgttca cgtcgcgtgt
      901 gaactcatca taataaaatc cgtagacggt gtagctgttg agcccgttcg tgaaggtcgc
      961 ttcaacgctg atcttgtcgg gatcgaaggg attgaggccg gtgttgggat cggcgagaaa
     1021 atcgtttacc tgctgatcaa cggatgcggg cagttgcatt cctatttcca gcttttcgaa
     1081 ctgtccgacc tgcggaagat gttcatccgg cgtgatgaac acgacgttaa aatcgggatt
     1141 ggcgatgaat gcatgaaagt atccgttgcc cgtgtaatca ccggcgtgaa aaccgggctg
     1201 caggtgcacg cggtccttcg ccgtcatgtt cgtttcactc gtttgtgtaa acgtgatgtg
     1261 atcgtaatag aacggggaga acgtttcgcc cgggccggag aaattatact ggccggatgt
     1321 aatgggcgct aagggatcat cgacgggcgt ataaggcgac tgctgcgcaa tgcagagttg
     1381 cgaagctgtg aacgcggcga aaacagacaa atattttttc attttacttt cccccgttct
     1441 ttttcagttg ctcattttcg gcctcaagcg ctttcaggcg gtcgttgatc tggagaatgt
     1501 aaagcatcat ctcttcgatc tgcttctgct gcgccgctgc tgtagcgctt accgacatgc
     1561 cgtccgattc gatctctttt tcgggccgga aaccggggag atgcctgttg atcttaacat
     1621 acatcccgac agaatcaagc ggcatcagcg tataggcaga atcgaaaact tcgtcctgcc
     1681 agtcgatgtt gctcaccacg acgtctttgc agagtatgcg tccctgtacc atcagtttgt
     1741 tcagcggatc gttgttgtat tgactcgaag ccgcaagacc gccgttcccg atgagaacgt
     1801 aattgctgta aatgcgcatc gcggttccgc cggtcatgtg cacgtaatcc tgctggttgt
     1861 cggcgctgcc ggtgttggga agaacattaa accagagcga gccgtcgctg ctgagcgaaa
     1921 tggctgcacc ggtgttggtg ttcgctccgc ccgtcgtgtg cataaacccg ttgctgttca
     1981 gttcggcgtt gaagccgacg tagccgagaa ttccacctgc cgcggcggga gcagcgtcaa
     2041 tgtttatcct ttgtattccc gaattgacct ggagcagcga tgacggggcc gtgctgccaa
     2101 tgcctacggt ccccagggtt cccgttccgc ttcccggccc gacgaacaaa gtcggtatcg
     2161 tgctgttgaa gccgatcatg agcgaattcg gaacattgtt cgtcatccac gaggaaacgg
     2221 aaacgttatt cggtccggaa ccgatcgcga tcgaataggc agcggaagga tcggtcgcca
     2281 cattcctgcc tatcgcaagg gaagaaaccg cccgtgcgcg gcctgatccg tagccgatca
     2341 gcaatccttt tacggagccg gcaggtgaag cggtgatgta gtttcccgtt cccgtgttat
     2401 tcacaccgac gatgacagag ctgtcgccga agtggatctc gctgtcaccg gccaacggaa
     2461 cgatgcgatg gatatacatc tgcccgctga cgcgctggtc gccgttcacg tcaagcttgt
     2521 actgcggcgc aaaaattccg atgcccacgt ttccgtttgt tcccgcgtta aggatcgtat
     2581 tgccgttgta gccgggtttg ctctgcacgc gcacgtcgcc tgtgagcgca tacatttcat
     2641 tcagcgttcc gccggtgaga ttgaggttgt tgttcactga aatgctgctg gctcctttga
     2701 tggtgtccgc ctgtaacttg ttcgcgagga taagcccgtt ggcataaagt gttccgttga
     2761 atttcgcgct gccgttcacc tccagcggga actgcggcgt actgacaccg atgcccacgt
     2821 aaccggtatt tacggtgtaa agattattcc cgttgtacgc ccagccggaa gctgcggaaa
     2881 tgcttgagaa atttccatta ccgtcgaggt acaggctgtc gttaccgggc agggcaagac
     2941 tgccgagcac gccgttgttg ttcgacgtta cgagcgtacg tccgttaccg ccgaggttgc
     3001 gcactttcag gtcgccgtcc tgtttgatgc gcattgcttc gctgtcgttc acgaaaaaga
     3061 caagcgggtt gttgttcgtc gttcctataa aatcgccgtt gccggtggca ttaccggcgg
     3121 tggaccattt gggcgcctgg gcggagagaa aaaacggcag gcagaggagg caaagggtaa
     3181 atccgggtga tttcattttt cgcaaggggt tatgcatgta agcggttctg tttcttttga
     3241 aaattaccgg tcttcagcaa gaaaaccggg caaagtttca gcatgattat acatgtccag
     3301 gtggaaatga attttcgatc attcacgttg cggagtagat ataaattaat agtttttata
     3361 aataaagata taaataagtt taacatatcc aaatgtaagt tttcctttta accggcgccg
     3421 ccgaaacgcg ttcctccttc gcgcctggac agggcgtagt agccgaactt cagaaaaaag
     3481 aaaagaaagg agaggaatga aaaacgggcg gcttaccgca gtaaatgccc tctcaccacc
     3541 tgcagcgtga tcagcagccc gtcgagcatg gcgatccagc cgaagatgcg tttcatcacc
     3601 gtggcggatg tgcgcttcgc taagaaggga ccgagtcgcc cgccccagat cacgccggga
     3661 atggtgaaca tcgcgaggtc ccacggaacg ccgccgaagt aaagagcatg aacaagcgca
     3721 agtagaatgg aatttaccga aagcagcacg acgccgagcc cgacggattt gttcgtatca
     3781 agtccgtaca ccagcatgca gaacgcagcg atgatctctc cttcgccgat cgccacccag
     3841 cctgtgatga gtccgccgat gaaggaaaca aggaagaccg gcagcatcgc tttcggcggc
     3901 agatcgtcgc cggaactttt tcttccggcc agcaccagcg tcagcattcc cacgaaaaat
     3961 gaaacgggcc cgaacatgct tttgatgagc atcgtctgcg ggtgaatgaa aaaagtggaa
     4021 acgacggagc cgaggatgag cgaagggatc gtccatttca gcagcttcag cggaacggtt
     4081 ttccgctgca cccatcctgt tgcgccgctg gtcatgccga cggcctgcgt cacgaacgtc
     4141 agcttcagcg cagaaacggg attgacatga tagatgaaca tgaacacggg gatgaaaata
     4201 attccgccgc cgatcgctgt catgtttccc acgattgctg cggccacggc aagaaggatg
     4261 atcggccagt tctccaggaa caatcctgcg ggatcgggaa aaatgaaaag ataaatgatc
     4321 agccaggcgg ctgtgaggat aagcaggaaa ggagaaaaaa aacgtgcggg ctgcaaggtc
     4381 agatcttctc tttcaggaat tttcccgtgt aggaattctt tgcttttgcc acctgctcgg
     4441 gcgttccttc gaaaacgatg ttgccgcctt cgtcaccgcc ttcgggaccg aggtcgatga
     4501 tccagtcggc gcatttgatc acgtccatgt tgtgctcgat gatgatgagc gaatgtccct
     4561 gcgcgatgag cgcgttgaat gcgtcgagca gcttgcgtat atcgtggaaa tgaagacccg
     4621 tcgtcggttc atcgaagatg aacagcgtgg gaggctggtt gctgagaccg aggaacgacg
     4681 caagcttgat gcgctgcgct tcgcccccgc tgagcgtgct ggacgactgc ccgagatgca
     4741 cgtagccgag gcccgtgtcc tgcagcggct gcaacttgga aacgatcctt ttccccgggt
     4801 tcgtgccgtc tccgaaaaaa agaatggctt cgtcgatcgt catttcgagg acgtccgaaa
     4861 tatttttccc gttgtactgc acttccagcg tttcttcctt gaagcgtttt cccttgcaga
     4921 cttcgcattg gaggtgaacg tcggccatga actgcatttc caccaccact tcgccttcgc
     4981 cttcgcacac ttcgcagcgt ccgccatcga cgttgaagga aaaatgcgac gctttatagc
     5041 cgcgcatttt cgaaagctgc tggtcggcat aaagtgcgcg gatgtcgtcg tacgctttta
     5101 cataagtcac cgggttcgag cgcgaggatt tcccgatcgg gttctggtcg acgagctcga
     5161 cggaagtgat gtggcggaaa tcgcctttga gcgcatcgaa ctgtcctgtc tgctctccgt
     5221 agccgccgag atgtttctgc acggcgggat aaaggatccg gcgcaccagc gtcgattttc
     5281 ccgagccgct cacgccggtg acgacggtca tcacgtcgag cgggaatttt acgtcgaggt
     5341 ttttcaggtt gttctcgcgt gcgcctttca cctcgatgaa gtttttccat ttccggcgtt
     5401 tggacggaac aggaattttc tcgcggtgcg tgaggtattt cgcggtgagg ctttccgctt
     5461 tcagcaggtc ttcgtgcgaa ccgttgaaca cgacttcgcc gccgagcgtt cccgccatcg
     5521 ggcccatgtc caccatcacg tcggaagacc tcatgatctc ttcatcgtgc tcgacgacga
     5581 tcaccgtgtt gccgatgtcg cgcagcgcga gcagcacgcg gatgaggcgt tccgtgtcgc
     5641 gcgggtgaag accgatgctc ggttcatcga gaatgtacat cgatccgacg agcgagctgc
     5701 cgagcgacgt ggcgaggttg atgcgctgcg attccccgcc cgagagcgaa gaggaaaggc
     5761 ggttcagcgt caggtagccg agaccaacgt ccccgagaaa acggaggcgg ttggtgatct
     5821 ccgtgagcag gcgtttcgcc accgcgcggt catgttcgtc cagcttcatc gcggtgaaaa
     5881 acttcacggc gtccttcacc tgcatgagca cgatgtcgct gatggaatga ccgttcacct
     5941 ttacgtagtt cgcatccttg cgcaggcgcg ttccgcggca gtcgggacag gtggttttcc
     6001 cgcggtagcg cgaaagcatc acgcggtact ggatcttgta gctctgctct tcgaggtgtt
     6061 tgaaaaattc attgagcccg tggaaatgct tgttgcctgt ccacaacaat tctttctgcg
     6121 cctgcgaaag ctcgttatac ggtttgtgaa taggaaaatt gaacttatag gcgttcatca
     6181 cgaggtgctc tttccattcg cccatctttt cgcctttcca gcaggcgatc gcttcttcgt
     6241 aaacggaaag atttttattc gggatgacaa ggtcgggatc gatgccgatc accgagccga
     6301 aaccttcgca cgtcttgcac gcgccgatcg ggttgttgaa cgaaaagaaa tgcacgttcg
     6361 gttcttcgaa cagcatgccg tcggcttcga accggttgct gaaatacaaa cggttcccgt
     6421 tttgcgttcc tgcagcagcc tgcttccctt ttgcgctttt gggatttgga ctttgcgatt
     6481 ggggattttc ctccgcaatc tccacaacac aatctccttc gccttcataa aaagctgtct
     6541 gcacggaatc cgcgatgcgg gaacggccgt cttcatcctg cgggttaacc gcgatgcggt
     6601 cgatgacgat gaaaatatcc tccttcgcgg aaagcgaaga aacgttgatc tcgttcaggc
     6661 ggagaatttc attcctgtaa aggatgcgtg tgaaaccctg ctggagcagg aggccgagat
     6721 gctctttcag cgatcgttct tttttctttt tgagcggcgc gagcagcagc actttcgttc
     6781 caccgggaag acccattacg gcatccacca catcggaaac cgaatgacgc ttcaccggtt
     6841 cgccggaaac gggagagtag gttttcccga tgcgcgcgaa cagcagcttg aggtaatcgt
     6901 agatctcggt ggacgtgccg acggtggaac gcgggttgcg tgtgttcact ttctgttcga
     6961 tggcaacggc cggcgaaatt cccttgatgt attccacagc cggtttttcg atcttgccga
     7021 gaaactgccg ggcataggcg gaaaggcttt ccacgtaacg ccgctgtcct tcggcgaaga
     7081 gtgtgtcgaa ggcgagcgag gatttccctg aacccgaaag cccggtgatc accacgaaac
     7141 ggttgcgcgg tatcgccagg tcgacattct tcaggttgtg catttttgca cccttgatga
     7201 cgatgtattg cttgggattg tatgtttcga tcgcttcgga caccctgtga atttgaggct
     7261 gcaaaattac ccaaaaaagc gccttgtcgc tgatgagaaa tgaataggta acgatccttt
     7321 gggaatttta accgggaaaa ggccgaggca aatttcaatg tatgcggaag agccgatcgc
     7381 gtggtaatga aagacatgcc ggttccctga accgatttgt taaaaaagcg gcacgggctt
     7441 tgacattacg agacaggaag gtatatttgt agaaacagtt aaaccggcaa aaaagtttaa
     7501 tccccgcgca acatttaagc aataagcccg ttataaccgt taacaccttc tatttattgt
     7561 tcacccggcc gccagtcaaa aaggcccaaa aacaggaaaa gtatcgctta gacctctacg
     7621 ttttcaacca gaccacagct gaaaacccgc aaaaaggagg ctactatgcg ttacgaccaa
     7681 ttgcaagacc aggaactggt cgacctgtac atcgccggga acgaaccctg ccttgagatc
     7741 ctgatcgccc gtcacaagaa gcgcgtcttc tcgtacatca tgatggtgct gcgcgacagg
     7801 catctttccg aagacatttt ccaggagacc tttatcaagg ttatccagac gctgaaacgc
     7861 ggaacctaca acgacgaagg gaaattcctt ccgtgggtgc tgcgtattgc gcacaacctc
     7921 atcatcgacc atttccggcg gcagaaacgc atgcccgtgg tgagcggggg agaggacttc
     7981 gatatttttt ccgtgctgcg cctgcgccag gggaatatcg aggacaagct gatcaaaacg
     8041 caggtgcgcg aagatcttcg ccgctgcatc gaaaagcttc cgcccgagca gcgtgaagtg
     8101 ctgatcatgc ggcattacct cgacatgagc ttcaaggaga tcgccgacag cacgagcgtg
     8161 agcatcaaca ccgcgctcgg ccgcatgcgc tatgcgctca tcaaccttcg ccggatgatg
     8221 gacaaaaaac acgttgcggt cccggcctga aattattgaa acgacaaatg agag
//
LOCUS       DASTSF010000086        23730 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1508202,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000086 DASTSF010000000
VERSION     DASTSF010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 23730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23730
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1508202"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..625
                     /locus_tag="VFU15_02450"
     CDS             <1..625
                     /locus_tag="VFU15_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010599719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEU4716658.1"
                     /translation="VETLSIDAPELVSGKVIELVSQKKGEMLVMEPKGDLIHMEFDIP
                     ARGLIGLRNNILTATAGEAIMAHRFKAFEPWKGNIPGRIAGVLIAKDKGTAVAYSIDK
                     LQDRGSFFVSPGEEIYGGQVVGENIRPDDMVVNLVTEKKLTNMRASGSDDKVSIAPAI
                     KFSLEEAMEYIQADEYVEVTPLNIRLRKIHLDPHERDRLKKKMTAES"
     gene            complement(691..1230)
                     /locus_tag="VFU15_02455"
     CDS             complement(691..1230)
                     /locus_tag="VFU15_02455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716659.1"
                     /translation="MKRFFYAAAVLMSISWMACSGNKPDAQEPPLPQGMKSIHLDHLG
                     FPLNIYIPDSTYYPTVDTSEVPGGAEIKIGSHFDILVNTAGAEESDITKMKSVIEAGD
                     ELPKTFTVTDSTTLVWSTNLGDLSMFHFFMIRKVGKDTYYVRDNMDNPDNQFKKEEID
                     KMMETAKTLHADPRFAPKS"
     gene            complement(1744..2955)
                     /locus_tag="VFU15_02460"
     CDS             complement(1744..2955)
                     /locus_tag="VFU15_02460"
                     /inference="COORDINATES: protein motif:HMM:NF013362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU4716660.1"
                     /translation="MRKRNEGNGPGSLTLLKDALENFTVKGFPADKALNETFRKYKVK
                     EEFRRSELARRLSGIIRFWRPIISATGIESFSTVKEMDELVSALIAWKKITSGKPPGD
                     VPASWKEKMTRYLSVRKMRESVPDWLDELGVKELGEEKWNVTIRALNKEPMLFLRTNT
                     LLCDRNKLIGELRNAGIQAVPAAENGEGVLLEKYENVFRLAQFHEGWFEVQDISSQLV
                     GDFCKAEPGMRVADACAGNGGKTLHLGALMNNRGKIVAMDVSAPKLAELRNRCTRAHL
                     DTVETRLIESGTLKKMEASFDRVLVDAPCTGSGVLRRNPDAKWRLQPEDISPLLKEQQ
                     DALENYSPLVKKNGFLIYSTCSVFPSEGEMQVQKFLSAHPGEWEKEEEMRIDPEKTGG
                     DGFYMARLKRI"
     gene            3085..4293
                     /locus_tag="VFU15_02465"
     CDS             3085..4293
                     /locus_tag="VFU15_02465"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEU4716661.1"
                     /translation="MKTEKRTGRSAYGMISLFLLLLLVVLFSGVIFPGTRKAFALPSP
                     PKPAPDSIVTISMSFVGDLMCHVPQINNARHADGTFDFSPSFKEIAPYISSADLAMGN
                     LETTCAGPWKSYGGYPAFNSPDAYIAAVKSAGFDFLCTANNHSMDTGEEGLLRTVGVV
                     RKNGLGSTGTFLSQRDRDSIRVLDVRGIKIAVLNYTYGTNGAYPSAEHKYMLNVADSA
                     LVHDDILKARSTGADIVLVFYHWGIENQRSPVPKQDTMFHWAADAGADLIIGSHPHVI
                     EPVKYFKTAANATLDSGFVAWSLGNFLSNQYWRYTDAGVILSLSLQKNFTTGKVSLAK
                     TSYLPTWVYRSYDPSLQEHVVVPAGWCGNDSLPAWINADSKQKMCEAFGDTQGMLGSA
                     VSGMELRSVK"
     gene            complement(4295..5794)
                     /locus_tag="VFU15_02470"
     CDS             complement(4295..5794)
                     /locus_tag="VFU15_02470"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEU4716662.1"
                     /translation="MYRKLPALLIFLFLFGEVKAQRDTVIFADDPVAAMLDSLAHNAY
                     ISRIPRQQTQRNNKFHFAEDSIPVYDNAVIESRLAKLNAQSPFDLVYNEDVQTYIDLY
                     TMKRRPLVEKMLGLSQLYYPIFEEKLDKYNLPLELKHLAVIESALNPSATSRAGARGL
                     WQFMYTTGSVYGLQMTSYVDERCDPYKSTEAACQLLQNLYSMFGDWQMVLAAYNAGPG
                     AVNKAIRRAGGGKKTYWEIRPYLPRETQAYVPAFIAANYVMTYHAEHNLFATAPRKVF
                     FDVDTVAVKQQVTFSQLEAVLGISYDELEFLNPMYKLGVIPVSFGEPYHLVLPVGQVG
                     AFVTNEEKIYDYIKTDTAMSALAVQEVKSTHVVKKGETITSIARKYKCSVTDIRSWNS
                     LRTNYLKPGQKLIIYTTVTPPPPQDNTASKDKPPSGGTTAKPKTPPASNTASASAGDK
                     YEYYTVKNGDNLWTIANKTGTTVDELKKLNGFGSKIVLHTGDRIKVKKL"
     gene            complement(5962..7389)
                     /gene="gatA"
                     /locus_tag="VFU15_02475"
     CDS             complement(5962..7389)
                     /gene="gatA"
                     /locus_tag="VFU15_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017257918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEU4716663.1"
                     /translation="MKNYSSLSSVREDLSAGNISCAELVEGYLQRIDEKKHLNAFLEV
                     FSESARASAKTVDAKIAEGKAGRLAGMVIALKDNICYRNHKVSASSKILQGFVSPYSS
                     TVVERLLAEDAIIIGRTNCDEFAMGSSNENSAFGNVRNPHDEQRVPGGSSGGSATAVA
                     AGLCLAALGTDTGGSIRQPASFCGIVGMKPTYGRVSRWGIIAYASSFDQVGPLTNNVD
                     DAALILEVISGADEFDSTLSTKPVSPYSQETGLAGKLKIAYLEDCFEKEGLDPEIKSR
                     LETIIAQLREQGHTVEPVRFPFLDYLVPAYYVLTTAEASSNLARYDGIRYGYRSPNAT
                     DLESTYKKSRSEGFGPEVKRRIMLGTFVLSAGYYDAYYSKGQKVRRLLRDKTQEIFNT
                     YDFILLPTTPGTAFGFGANSADPIKMYLEDIFTVQANLTGFPAISVPAGKNSEGLPFG
                     VQLMGKRFDESGLFAFAKHVHRMQK"
     gene            complement(7470..7661)
                     /gene="tatA"
                     /locus_tag="VFU15_02480"
     CDS             complement(7470..7661)
                     /gene="tatA"
                     /locus_tag="VFU15_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008236392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEU4716664.1"
                     /translation="MTSFIILGFAGLGGPELIVILLIVLLLFGGRKIPEFMRGLGKGV
                     KEFKDASKGVDSEQADKKD"
     gene            complement(7728..8507)
                     /locus_tag="VFU15_02485"
     CDS             complement(7728..8507)
                     /locus_tag="VFU15_02485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716665.1"
                     /translation="MKKPIAFFVLFAICLVSASCRKALKDVNDYFPKVKTVSATVQAN
                     GSVMISGEIESDGADPIQYLGFCCGTHPDPRITDNQVIVQRDGNSFSAVLSGFSADSV
                     YYFRSWAANSYGYAYGNVISLDSIAAVPVTPPCSLTMNTVDIGGSTGTHTYSSVSTPT
                     SYMGIWQFTGNTATGPTVDFSFGSAIATGIYTTTLNTSPAAGQVYVDFTDGFIGGALN
                     SGSSVYVNTVSPGVYDITICSAPWQYNSSTFYFKTHLTAPH"
     gene            complement(8519..9139)
                     /locus_tag="VFU15_02490"
     CDS             complement(8519..9139)
                     /locus_tag="VFU15_02490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716666.1"
                     /translation="MRYLLFTMALLLLLCRVSRAQIGLRVFNYRPTGELGFVMKPLFS
                     AELSCIAPFEDGRWRTQFSLTYLRLKPRMDTFPVYGVVSSGSTGTTVYPGTQSFQKYS
                     IVQLSGGGDFAFIDKDPFYVFAGADIVVGGASVEYTSQVQTIKDESYSGGGILAGFRF
                     RLGAEYDVNDQVGIIASANRNYLFISEPAGLYGANDYGIGLRYSFH"
     gene            complement(9257..10000)
                     /locus_tag="VFU15_02495"
     CDS             complement(9257..10000)
                     /locus_tag="VFU15_02495"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015331959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEU4716667.1"
                     /translation="MNTRREFLFKSFLAGIGISFLGKNSLNANPAQANFLELIEDSTG
                     PYTLPLLPYAYDALEPTIDMQTMQIHHTKHHQAYVNNLNKALETIKDAPSNLDDLLKN
                     VSKYPASIRNNGGGHWNHSLFWTLMKPVSPDAVNMPTGKLAEAINSTFGSLDKFKELF
                     ANAGKERFGSGWAWLIVGKDKKLAICSTPNQDNPIMDIAETKGTPILGIDVWEHAYYL
                     KYQNKRPDYITAWWNVVNWEKSAELFDKA"
     gene            complement(10142..10660)
                     /locus_tag="VFU15_02500"
     CDS             complement(10142..10660)
                     /locus_tag="VFU15_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002697632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEU4716668.1"
                     /translation="MEKSSFTVTAGNKQHKLVLDEAGKWTGSIDGKKFSCDVISSGHG
                     RFHVIRDNRSYEVEIEKADVASKTVTVKVNGRSYPLQVKDKYDELLHSLGMDRTSGAK
                     INDLKAPMPGLVLEVKVNEGQEVKKGDAILVLEAMKMENILKSPADVKVKKVSVKKGA
                     AVEKNQVLVQFA"
     gene            10965..13709
                     /locus_tag="VFU15_02505"
     CDS             10965..13709
                     /locus_tag="VFU15_02505"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEU4716669.1"
                     /translation="MYKYIFPATLLVFLFACGHQQQTTASKPGPRNPHMQATDKPVID
                     PNLNAESRSDYNPSFTRVSDLLHTKLDVSFDWNKRYMYGKATITARPYFYPSSTLELD
                     ARGMDIKRVAMVNRGISDAGMVTDDTTALKYTYDQKKLKIDLGRVFSKEQEYTVFIDY
                     ISKPDELKDLGGSAAISEDKGLYFINPDGSNPNSPKQIWTQGETQSNCVWMPTIDRPN
                     ERMTDEIYMRVEDKYKTLSNGVLAESKENGDGTRTDHWRMDLPHAPYLVMMAVGDFAI
                     VKDKWHDKEVSYYVEPQFEPYARQTFGKTPDMIDFFSKKLGVDYVWPKYSQVCARDYV
                     SGAMENTSATLHGDFVQRDDREYLDRTYEDYISHELFHQWFGDLVTCESWSNLPLNES
                     FATYGEYLWDEHEYGKETADYGHYNSLQGYLDEANAGHATPDDPGKREPLIRYYYTDR
                     EDMFDGHSYNKGGQVLHLLRQYVGDDAFFASLHLYLETNKFKAAEADNLRLAFEETTG
                     QDLHWFFNEWFYSPGHPELDISYNYDAANQKERVIIKQTQDRSNGTPVFRIPLSVDVY
                     NGDKVTREECIVKSTCDTFWFASPAKPDFVNVDAQKTLPCFKIDHHTAQEWAFLYNHS
                     KLYVDRVEAIDNLAPEGLGGEVTMMMNNKPVTSRHGAMAGPDSAIAVQTVTKALDDEF
                     WAIRQMACEELGDYADHVKDKLTALAKKDEKSMVRAAAISALNAGSKSDDLKSVYLDA
                     LNDRSYTVITRALVALADHFPDTGMDEAKKRENDPARSMKIAVANAYSHCGTEAQQPW
                     FEKTMNTLYGVTQREFCYVYTAFLVRKCSPAATEKAIPAVHALYTDNHSPTAIQGAQT
                     IIFSLDYVYAKRLDNLQKKINSTKKKSPAYAKMLADKADMQHLLGVLDEEKKKMTADK
                     "
     gene            complement(13722..14234)
                     /gene="rnhA"
                     /locus_tag="VFU15_02510"
     CDS             complement(13722..14234)
                     /gene="rnhA"
                     /locus_tag="VFU15_02510"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HEU4716670.1"
                     /translation="MTPANPATTTIFTAEFMSHNITVYTDGASRGNPGPGGYGVILMS
                     GNHRKELSQGFRTTTNNRMELLSVIVALESLKVPGSHVTIYSDSRYVVDSVEKKWVFG
                     WQQKGFKGKKNADLWMRFLKIFPKHHVKFVWVKGHADNPFNERCDELAVAAALGSHLH
                     VDHGYEELPE"
     gene            14304..17138
                     /locus_tag="VFU15_02515"
     CDS             14304..17138
                     /locus_tag="VFU15_02515"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEU4716671.1"
                     /translation="MKQVYTHVFLVLLSAFLIGEKANAQVNVTITSFNDVLCNGGNDG
                     SATASASGGTGPYSYSWAPSGGTNATAGGLVAGVYTVTATDATLASATATIAITQPTL
                     LSISTSSVPATCGFPNGSASGSPSGGTPPYTYSWAPSGATSATATNLASGSYTITVTD
                     SHGCVVTGVVAVPNYPAPISNAGPDQNICPGGTTVLSGSLSGGSPPYTYSWSGPSGFF
                     SNLLSPSVSPATTSTYTFTVTDSNGCSSSDVVTVFIAPLLNLTFNSSPATCSQTNGMI
                     SVTPSGGVSPYTFLWGNNSTNDTLFNVSAGLYSVTVTDAGGCSNSGQGAVSNATGPVI
                     TLSGVTNPSCYGSTNGSATANVTGNAPPYSYSWNTQPVQTTPTAGALPSGTYFCTVTD
                     TNNCQSSVSVTLVQPPQLYLGVQQLAPANGNSSGSANAWAGGGNPPYTFAWSNSMSGD
                     TITGLTSGAYSVTVTDNSGCTSQGSVFVANINATIVTGRIYADINSNCVFDAGDMPIA
                     NQYVPFYPAQVYAMSDSSGMYTAYVTTTGTQTEYSNFNWMSPYTTGFCPATSTRVISI
                     PALYDTIPGMDFARQDSANVQDLRVSLSFYQAPRAGFLQTAAIYYQNVGTVPVSNTTV
                     NFVFDSILTYVSSSAPPSVNQPAYLEWNTGTLQPGESHMLLATLQVPTIQNGGWIGRP
                     LFYSAAINPNASDVTPVDNGDDAFSHIIASYDPNEKTCYAAGMDSLGNILPSDSVLSY
                     TIHFQNTGTDTAFNIYVIDTLSPYLDPVTVIPGASSHPYSFEMYGNGVMRFDFYNILL
                     PDSNHDSEGSNGFVKYRVKVRPNTAPGSTIDNTGYIYFDFNPAVVTNTTSNTIWNNVP
                     TAVHENSTSGISAFPNPFDESTTIVLPQQYAGKQNQLVITDAAGRVVRSESFTTSSYT
                     FARNTLDSGMYFLTVYSDGKKTGVTKIVVQ"
     gene            17357..18055
                     /locus_tag="VFU15_02520"
     CDS             17357..18055
                     /locus_tag="VFU15_02520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716672.1"
                     /translation="MFGIIGGPGWLFVLLVLIDIGFVFAMTILWLRSMNNTMDACPVY
                     SRTAEPALVWLTLIPLFGFVWQFIAVFNVSNSLAKEYARRKWSSEENRPALETGIIAC
                     IAVVVVTFVRLFFPIHPGIGFIGSMVVAFCMYRHMDRMQAYRERLEKERDPTLQFGQI
                     TPKKEVPNPFADLYPKAGVSYPPAAIVHHQQKNTASSMKNRSTPLPGNTSSYGEVPPE
                     YSPWMPKPDKKDEK"
     gene            18114..18689
                     /locus_tag="VFU15_02525"
     CDS             18114..18689
                     /locus_tag="VFU15_02525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716673.1"
                     /translation="MSVLSFYGIMWLVLFAGWAGTTAWSNVVCIRALSLTQPPARRME
                     PQNVWLTFVPLFNLYWQFVVTYAVADSLGDEMRRRNYIPRQARPGLSQGMTACIFLCF
                     AVVPAMGLLIANISNIVRIIHLQTVSRFSDELEELIRVQNAYAPAVPQEMILQQQVNP
                     EEAEKKEKENPERFMPPPTPEDEWKRWGRKD"
     gene            18725..19102
                     /locus_tag="VFU15_02530"
     CDS             18725..19102
                     /locus_tag="VFU15_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group III truncated hemoglobin"
                     /protein_id="HEU4716674.1"
                     /translation="MKDIENRNDVEQLVGLFYKKAIHDTVIGHFFTAVIPISWEKHIP
                     LIVSFWESILFGNASYRGNPMEKHIELSRLSKMEAHHFERWLKLWKETTDENFAGPKA
                     NEAKVRAEAIARLMLHNVQAAHN"
     gene            complement(19204..20151)
                     /locus_tag="VFU15_02535"
     CDS             complement(19204..20151)
                     /locus_tag="VFU15_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020527504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HEU4716675.1"
                     /translation="MGKPFHIQGQTVKAGEHAQLILNIYKLPTHTLIEIPVFIFNGKE
                     PGPTILFLAGMHGDEINGIEVVRQLIKREDVQKPRCGCIVAIPVINIISFLAGSRDLP
                     DGRDLNRCFPGSKNGSLGSRIAHDLMETIIPQIDFGFDFHTGGAKISNYPQLRCVFDD
                     PKNLRLGKIFAPLMVINSPFRDNTLRQAAAKLGKQILVFEGGESRRFDYESVREGIHG
                     CVRVMNHFGMIKANVAQKSTVLLKKSSWIRAKSSGLFHTTKKNGGKVKKGERVGIICD
                     PYGEVEHELFAPANGFIVGVNNQPVVNQGDALIHVGREK"
     gene            complement(20254..21141)
                     /gene="rimK"
                     /locus_tag="VFU15_02540"
     CDS             complement(20254..21141)
                     /gene="rimK"
                     /locus_tag="VFU15_02540"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017733824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HEU4716676.1"
                     /translation="MRIGVLSRNANLYSTNRLVEAGRERGHDMLVIDHQKCVLVIEQN
                     HPRIFYKGEELTGVNAIIPRIGASVTFYGAAVVRQFEQMRVFSTVESQALVRSRDKLR
                     SLQLLAKAGLGMPKTAFASSPKDIDNIIRQVGGTPVVIKLLEGTQGIGVILAETHNSA
                     KSVIEAFLDVEVSILVQEFIAESKGTDIRAFIVDGQIVGAMRRQGAEGDFRSNLHRGG
                     KASLVTLSAEEKATAIKAVKKLGLAIAGVDMLQSNRGPLVMEVNSSPGLEGIEGATGV
                     DIAGKIIEYVERNEFHTHL"
     gene            complement(21145..21588)
                     /locus_tag="VFU15_02545"
     CDS             complement(21145..21588)
                     /locus_tag="VFU15_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019946094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RimK/LysX family protein"
                     /protein_id="HEU4716677.1"
                     /translation="MKKRAKIRPVETVGRREVVDFPDLGLFGLTAKIDTGANTTALHC
                     HHVRLENGVLHFRLLDESHPEYQDREHVFQKFEQKLIRSSFGEQEMRYIVRTRIRIGK
                     RTIRSIVSLTDRKNMKYPVLIGRRLLKHRFVVDVTKLNSLSSTNH"
     gene            complement(21604..22530)
                     /locus_tag="VFU15_02550"
     CDS             complement(21604..22530)
                     /locus_tag="VFU15_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEU4716678.1"
                     /translation="MIGAILFGVFIFFVLLLSFVTIQQGMVGVTTIFGKYNRVLRPGL
                     NFKVPFVERVYKRISIQNRSAELGFQAVTIDQANVNFTAMLLYAVVNQDEQTIQNVAF
                     KFVNDQNLMQALVRSIEGSVRAFVATKKQSEVLSLRRDIVEAVKEQLDQTLEGWGYHL
                     IDLQLNDITFDDEVMRSMAKVVASNNLKAAAENEGQALLITKTKAAEAEGNAIKIAAQ
                     AEKDAAQLRGQGVALFREEVAKGMSQAAKMMTDANLDASLILFSMWTEAIKNFAEHGK
                     GNVIFLDGSNEGMERTMRQMMAIGQMDLRDKK"
     gene            complement(22739..23170)
                     /locus_tag="VFU15_02555"
     CDS             complement(22739..23170)
                     /locus_tag="VFU15_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEU4716679.1"
                     /translation="MNVQEQIKKYITSQPEPKRKDMQELHRLMLRVLPKCKLWFDSGK
                     NSENKTVSNPTIGYGFQTIKYANGKTREFFQIGISGNKTGISVYILGIKDRTYIAKTY
                     GKKLGRANVTGYCIRFKTLDDINIDILEAAIRYGVEVSAGA"
BASE COUNT         5385 a         6241 c         6364 g         5740 t
ORIGIN      
        1 ggtggaaaca ctgagcatcg atgcgccgga gctcgtctcc ggaaaagtga tcgagctggt
       61 gagccagaaa aaaggagaga tgctggtgat ggaacccaaa ggcgatctca tccacatgga
      121 attcgacatt cccgcacgcg gcctgatcgg cttgcggaac aatatactca ccgcaacggc
      181 aggcgaggcg atcatggcgc accgcttcaa agcgttcgaa ccctggaagg gaaatattcc
      241 cggccgcatc gcgggtgtgc tgatcgcgaa agataaagga acagccgtcg cgtattcgat
      301 cgacaagctc caggaccgcg gatcattttt cgtttcgcct ggcgaggaaa tttacggcgg
      361 acaggtagta ggtgaaaaca tccgcccgga tgacatggtc gtaaacctgg tgacggagaa
      421 gaagctcacg aacatgcggg cgtccggcag cgacgataaa gtcagcattg cgccggccat
      481 caaattctcg ctcgaggaag cgatggaata tatccaggct gacgaatacg tggaagtgac
      541 gccgctgaac atccgcctcc gcaagatcca tctcgatccg catgagcgcg atcgtctcaa
      601 gaaaaagatg acggcggaat cctgatcgtc gattgaaccc aaaaagaaag gccggaaatt
      661 ttccggcctt tttcatgagg tgaaacttca tcacgacttc ggcgcgaagc ggggatccgc
      721 atgaagcgtt ttcgccgttt ccatcatctt gtcgatctct tccttcttga actggttgtc
      781 cgggttgtcc atgttgtcgc ggacgtagta ggtgtctttc cccactttgc ggatcatgaa
      841 gaagtggaac atggacaaat cgccgaggtt ggtcgaccac acgagcgtcg tcgagtcggt
      901 tacggtaaat gtcttgggaa gctcgtcgcc ggcttcgatc actgatttca ttttcgtgat
      961 atcggattct tcagcgccgg cggtgttgac gagaatgtcg aaatgcgatc cgatctttat
     1021 ctcggcgccg cccggtactt cacttgtgtc gacggtagga taatacgtgg aatcgggaat
     1081 gtatatgttg agcgggaacc cgaggtgatc caggtggatc gatttcattc cctggggcag
     1141 cggtggttcc tgtgcgtcag gtttgttgcc ggaacatgcc atccatgaga tggacattaa
     1201 aacagcggcg gcgtaaaaga aacgtttcat gattttggtt ttagggagtg acggaatcaa
     1261 agatatgaat tcaggtataa aagaaaagtc ccgactgagc cgggaccttc ttcaaaagtt
     1321 tcgaagaatt acttcttctt agctttctta gcagcttttt tcttcggagc tgcttttttc
     1381 ttagcagctt tctttttcgg agctgctttt ttggttgctt tcttagccat ggtttttggt
     1441 ttttgaaaat ttaattgtga cgaagtaaca aaaaaataat cagcgaaaaa aaattatcaa
     1501 catctatttt ttcaacacgg ttttgttaat aaggctgaac gtgtgcgcat tgcagaattt
     1561 tttttcgtga cgcatcagcg gttttgtttg aaaaaaaaat ttgcagaaaa aaatccgatc
     1621 tcgtacaacg cagtgctggc aaaggtttca gcgttccggt attttctttc agttgaattt
     1681 tttttctgcc gcgaatcagg atcacgtttc gttttttgaa aaaaggaaga gaaagaagac
     1741 gcgtcagatt cttttcagtc gcgccatata aaaaccgtcg ccgcctgttt tttccggatc
     1801 gatgcgcatc tcttcctctt tttcccattc acccggatgt gcggaaagaa atttctgcac
     1861 ctgcatttct ccttccgaag gaaaaacgct gcatgtcgag taaatgagaa agccgttctt
     1921 ctttaccagg ggggaataat tttccagcgc atcctgctgc tccttcagca gcggggaaat
     1981 atcttccggc tgcaggcgcc attttgcatc ggggttcctg cgcagcacgc ccgagcccgt
     2041 gcagggtgcg tcgaccagca cacggtcgaa cgaggcttcc atttttttca gcgttccgct
     2101 ttcgatgagc cgggtctcga cagtgtcgag gtgcgcgcgc gtgcagcggt ttcgcagctc
     2161 cgccagcttc ggtgcggaaa catccatcgc cacgatcttt ccccggttgt tcatcagcgc
     2221 accgaggtga agtgtttttc ctccgttgcc tgcacaggcg tccgccacgc gcatgcccgg
     2281 ttctgctttg cagaaatcac cgaccagctg cgaggagatg tcctgcactt cgaaccagcc
     2341 ttcatggaat tgtgccagcc ggaacacgtt ctcgtatttt tccagcagca cgccttcgcc
     2401 gttttccgca gcaggcactg cctggatacc ggcgttgcgc agctccccga tcagcttgtt
     2461 gcggtcgcac agcaatgtgt tcgtgcggag gaaaagcatc ggctctttgt tgagcgcgcg
     2521 gatcgtcacg ttccattttt cttcccccag ctctttaacg cccagctcat cgagccagtc
     2581 gggaaccgac tcgcgcatct tccgcacgct gaggtagcgc gtcatttttt ctttccatga
     2641 cgcaggaaca tctcccggcg gttttccgga tgtgatcttt ttccaggcga tcagcgctga
     2701 aacgagttcg tccatttctt tcaccgtcga aaaggattcg atccccgtgg cggaaataat
     2761 gggccgccag aaacggatga tcccggaaag ccgtcgcgcc agctcgctgc ggcggaactc
     2821 ttccttcact ttatacttgc ggaatgtttc attcaacgct ttgtcggcgg gaaacccttt
     2881 caccgtaaaa ttttccagcg catctttcag caaggtgagc gagcccggac cgtttccttc
     2941 atttctcttt cgcataaggt caaagatagt tcatgcggcg tgcggcgtga cggatattcg
     3001 caggaaggaa cacaacggca cggaatatgg acaactgttc agcggatgtc cgggcggaat
     3061 gtccgcgtgc tatctttaca gtatatgaaa acagaaaagc gtaccggccg ctctgcatac
     3121 gggatgatct cactcttcct cctgcttctg ctcgttgttc ttttttccgg ggtgatcttt
     3181 cccggaacgc ggaaagcctt cgccctgcct tcgccaccga aaccggcacc ggattccatt
     3241 gttacgatca gcatgagctt tgtcggcgac ctgatgtgcc atgttccgca gatcaacaat
     3301 gcacggcatg cggacggtac gttcgatttt tccccgagct tcaaagagat cgcaccttac
     3361 atttcatccg ccgacctcgc gatgggaaac ctggagacga catgcgcggg tccgtggaaa
     3421 agctatggcg gttacccggc gttcaattca ccggatgcgt atatcgccgc cgtcaaaagt
     3481 gcgggctttg atttcttatg caccgcgaac aaccacagca tggacaccgg tgaagagggt
     3541 ttgctgcgca cggtgggcgt cgtcaggaaa aacgggctgg gctccaccgg gacttttttg
     3601 tcgcagcgcg accgtgattc gatccgtgtg ctggatgtcc gtggaataaa gatcgcggtg
     3661 ctgaattata cgtacggaac caacggcgca tatccttccg cggagcacaa atacatgctc
     3721 aacgttgccg acagcgcgct cgtgcacgac gacattctga aggcgagaag cacgggagcc
     3781 gatattgttc ttgtttttta tcattgggga atcgagaacc agcgttcgcc cgttccgaaa
     3841 caggacacga tgttccactg ggcggccgat gccggcgccg atctcatcat cggttcgcat
     3901 ccgcacgtga tcgagccggt gaagtatttc aaaaccgctg caaacgcaac gcttgattcg
     3961 ggtttcgtgg cgtggtcgct cggaaatttt ctttccaacc agtactggcg ttacacggat
     4021 gcgggcgtga tcctctcgct ttcgctgcag aagaatttta ccaccggaaa agtttcactg
     4081 gcgaaaacat cctacctgcc gacgtgggtg taccggtcgt atgatccttc actgcaggag
     4141 cacgttgttg tgccggccgg ctggtgcggg aatgattcgc tgcccgcatg gatcaatgcg
     4201 gattcaaagc agaaaatgtg cgaagccttc ggagacacgc agggcatgct cggttcggca
     4261 gtttcaggaa tggagctgag atcggtgaaa tagctcagag ctttttcact ttgatgcgat
     4321 cgccggtgtg aagaacgatc ttgcttccga aaccattcag ctttttcagc tcatcgacgg
     4381 ttgtgccggt cttgttcgcg atcgtccaga gattgtcgcc gttcttcacg gtgtaatatt
     4441 catacttgtc gcctgcagaa gctgatgcgg tattcgaagc cggcggcgtt ttgggtttgg
     4501 ccgtcgttcc cccggaagga ggtttgtctt tcgatgcggt attatcctgg ggcggcggcg
     4561 gagtaaccgt tgtgtagatg atgagcttct gacccggctt gaggtaattt gtcctgaggc
     4621 tgttccacga gcgtatatcc gttaccgagc atttgtactt gcgcgcgatg gaagtgatcg
     4681 tttctccttt cttcacgacg tgggtggact tcacttcctg cacggcaaga gccgacattg
     4741 cggtatccgt tttgatgtaa tcgtagatct tttcttcgtt cgttacaaat gcgcccacct
     4801 gcccgacagg aagaacgaga tggtaaggct ctccgaatga aacagggatc acgccgagct
     4861 tgtacatcgg gttgaggaat tcgagctcat cgtatgagat gccgagcacg gcttcgagct
     4921 gtgagaatgt cacctgctgt tttacggcaa cagtgtcgac gtcgaaaaat acttttcgcg
     4981 gagccgtcgc aaaaagattg tgctccgcat gataagtcat cacgtagttg gccgcgatga
     5041 atgcaggaac gtacgcctgt gtttcacgcg gcaggtaagg acgtatttcc cagtatgttt
     5101 tttttcctcc gcccgcgcgc ctgatcgctt tgtttacggc tccgggtcct gcattgtacg
     5161 cagcgagcac catctgccag tcgccgaaca tcgagtacag gttctgcagc agctggcagg
     5221 cagcttcggt cgacttgtac ggatcacagc gttcatcgac gtatgacgtc atctgtaatc
     5281 cgtaaacact gcccgtggtg tacatgaact gccagagtcc gcgcgcgccg gcccgtgaag
     5341 tagcggaagg attgagcgcg gattcgatga cggcgagatg tttcagctcg agcggaaggt
     5401 tgtatttgtc gagcttttct tcgaaaatgg gataatacag ctgagaaagc ccgagcattt
     5461 tttcaacgag agggcgccgc ttcatcgtgt acaggtcgat gtaggtctgc acgtcttcat
     5521 tgtagacaag atcgaacggc gactgcgcat tcagttttgc gagacgggat tcgataacgg
     5581 cattatcata aacgggaatg gaatcttccg caaaatggaa tttattgttg cgttgcgtct
     5641 gctgccgcgg tatcctgctg atgtaagcgt tatgtgcgag gctgtcgagc atagccgcaa
     5701 cgggatcatc ggcaaagatg acggtgtcgc gctgcgcttt cacctcaccg aagagaaaaa
     5761 gaaaaatgag cagggccggg agctttctgt acatcagcac ggggacttta attgttatac
     5821 gcacaatgca cggtaaaagt gtgcatttat acaaatatcg catctgcaac cgattatatc
     5881 aacgaccaga acgggggtta tcccctttga aatgttaaaa acagccagga tacaggtgga
     5941 gacggccctg tgcgccatgc atcacttctg catcctgtgc acgtgctttg caaaagcgaa
     6001 gagcccgctt tcatcgaaac gttttcccat gagctgcacg ccgaacggaa gaccttcgct
     6061 gttctttcct gcgggaacgg aaatagcggg gaagccggtg aggtttgcct gcacagtgaa
     6121 aatatcttcg aggtacattt tgatcgggtc ggcggaattg gcaccgaaac cgaacgctgt
     6181 gccgggagtg gtcggaagaa gaataaaatc gtaagtgttg aagatctcct gcgttttatc
     6241 gcggagcagg cgccgcactt tctgcccttt ggagtaatag gcgtcgtaat atcccgcgct
     6301 gagcacgaat gttccgagca tgatgcgccg ttttacttcc gggccgaaac cttcgctgcg
     6361 cgattttttg tacgtcgatt caaggtccgt tgcgttcgga cttctgtacc cgtaacggat
     6421 gccgtcgtaa cgagcgagat tggaagaagc ttccgccgtc gtcagcacat agtaagcggg
     6481 aacgaggtaa tcaaggaaag gaaaacgcac gggttccacc gtgtgtccct gttcacgcaa
     6541 ttgcgcgatg atcgtttcga gacgcgattt tatttcagga tcgagacctt ctttttcgaa
     6601 gcagtcttcg agataagcga tcttcagctt gcctgccagc ccggtttcct gcgaatacgg
     6661 ggaaacaggc ttcgtggaaa gtgtgctgtc gaattcatca gcgcccgaga tcacttcgag
     6721 aatgagcgcc gcatcgtcga cgttattcgt gagcggaccc acctggtcga aagaagaggc
     6781 gtacgcaata attccccagc gggaaacgcg tccgtacgtg ggtttcattc cgacaatccc
     6841 gcagaacgaa gccggctggc ggatcgatcc gccggtgtcc gtgccgagtg cggcgagaca
     6901 aagtccggca gcaactgcag tggcggaacc gccggaagaa ccgccgggaa cgcgttgctc
     6961 atcgtgcggg ttgcgcacat tcccgaacgc ggaattttca ttcgaggaac ccatcgcgaa
     7021 ctcgtcgcag ttggtgcgcc cgatgatgat cgcatcttcc gcgagcagcc gttcaacgac
     7081 ggtgctgctg taaggagaaa cgaatccctg caggattttc gaggaagcgg aaactttgtg
     7141 gttcctgtag cagatgttgt ccttcagcgc gatgaccatt cccgcgaggc ggcccgcttt
     7201 tccttcggcg atcttcgcat cgaccgtttt cgcggaggca cgtgcgcttt cggaaaaaac
     7261 ttcgaggaaa gcattgaggt gcttcttctc atcgatccgc tgcaggtagc cttctaccag
     7321 ttccgcgcag gaaatgttgc cggcggaaag atcttcacga acagatgaaa gagaagaata
     7381 gttcttcacg ggaatgctga atgttgaatg cagaatgctg aatttttccg aacgcagaat
     7441 tcagaatccg tgccgggatc cggaagggat cagtcttttt tgtcggcctg ctcgctgtcc
     7501 acgcctttcg aagcgtcttt gaattctttt acgcctttac cgagaccgcg catgaattca
     7561 ggaattttac ggccaccgaa cagcaacagc acgataagaa gaattacgat cagctcaggg
     7621 ccacctaaac ccgcaaaacc gagtatgata aaagatgtca tgttgagtag tttctagtct
     7681 gcaaagatac gtaaaaataa gcccctgcac cagcagaata aaagccttca atgaggtgcc
     7741 gtgaggtggg ttttgaagta aaaagtggac gaattatact gccacggggc gctgcagatc
     7801 gtaatatcat agacacccgg gctgactgtg tttacgtata cgctgctacc gctgttcagc
     7861 gcccccccga taaatccgtc ggtaaagtcg acgtatacct ggccggctgc gggacttgta
     7921 ttcagtgtcg tcgtgtaaat tcctgtcgca atggcggaac cgaatgaaaa atcgacggtc
     7981 ggcccggtgg ctgtattccc cgtgaactgc cagataccca tgtagcttgt gggcgtgctt
     8041 acagaggaat acgtatgcgt gccggtgctt cccccgatat cgaccgtgtt catggtcaac
     8101 gagcagggcg gggtgacagg tacggcggct atgctgtcga ggctgatgac attgccgtaa
     8161 gcgtacccgt atgaatttgc cgcccacgag cgaaaataat aaacggaatc ggcgctgaac
     8221 ccggacaata cagccgaaaa gctgttcccg tcccgctgaa cgatcacctg gttgtcggtg
     8281 atgcgcggat ccggatgtgt cccgcagcaa aagccgaggt actggatcgg gtctgccccg
     8341 tcggattcaa tttctccgga gatcattact gatccattcg cctgcacagt cgcggaaacg
     8401 gttttcactt ttggaaaata atcgttcaca tccttgagtg ctttacggca tgaagcggaa
     8461 acaaggcata ttgcgaacag gacgaaaaag gcgattggtt ttttcatgac ggacagcatt
     8521 aatggaatga ataacgaagg ccgatcccat aatcgttggc accgtacaac ccggccggtt
     8581 cgcttatgaa taaataattc ctgttcgcgc tggcgatgat tccgacctgg tcgttcacat
     8641 cgtattcggc gcccagccgg aaacggaacc cggcaagtat tcctccgccc gaatagcttt
     8701 catccttgat cgtctgcacc tgcgaggtgt attccacgct ggctccgccc acaacaatat
     8761 ccgcgcctgc gaatacgtaa aaaggatctt tgtcaatgaa ggcaaaatca ccgccaccgc
     8821 tgagctgcac gatggaatat ttctgaaacg attgcgtacc tggataaacc gttgtgcccg
     8881 tgctgccgga acttaccacc ccgtatacgg gaaacgtgtc catccggggt ttcaggcgga
     8941 gatacgtgag cgagaattgc gtgcgccacc ggccgtcctc aaaaggtgcg atgcagctga
     9001 gttctgcaga aaacaaaggt ttcatcacga agccaagttc gccggtaggg cggtagttga
     9061 atacacgcag acctatctgt gcgcgcgaga cacggcaaag caggagcagc aatgccatcg
     9121 tgaagaggag gtaacgcatt gggttttgag gtagaaaaat aaaaataaaa aaattccacg
     9181 ttcacatggg cagggaaatt acctccacgc ctgtgcgcgt gacatcaacg atgacccgga
     9241 aaaaaagatg cagggcttac gctttgtcaa aaagctccgc cgatttttcc cagttcacca
     9301 cgttccacca tgcggtgatg taatccgggc gcttattctg gtacttgaga taatacgcgt
     9361 gttcccacac gtcgatgccg agaatgggcg tgccttttgt ttcggcgatg tccatgatcg
     9421 ggttgtcctg gttcggtgtg gagcagatcg cgagcttttt gtcttttccc acgatgagcc
     9481 atgcccagcc ggaaccgaaa cgttctttgc cggcgttggc gaaaagctct ttgaatttat
     9541 cgagggagcc gaacgtgctg ttgatcgctt cggcaagttt tcccgtgggc atgttcaccg
     9601 catccggtga aacaggcttc atgagcgtcc agaaaagcga atggttccag tgaccgccac
     9661 cattgttgcg gatcgatgcg ggatacttcg aaacattctt cagcaggtcg tcgaggttcg
     9721 acggggcatc cttgatcgtt tcgagcgctt tgttgaggtt gttgacatac gcctggtgat
     9781 gcttcgtgtg gtggatctgc atcgtctgca tgtcaatggt cggttcgagc gcatcgtagg
     9841 cgtacggcaa caggggcagc gtgtacggac ctgttgaatc ctcgatgagc tcaaggaagt
     9901 ttgcctgcgc cgggttggcg ttcagcgagt ttttcccgag gaaggaaatg ccgattcctg
     9961 cgagaaagct tttgaaaaga aattcgcgac gagtgttcat gagaatgtgt gttaagggag
    10021 gagaatggag tggatgattt ccatcgcttc tcccggttta tgaattgaaa tgaacgttca
    10081 cttacagaaa tgtctctgca atccgtttca actcttcccc tgttgccttt ctccgtcctg
    10141 tctacgcaaa ttgcacgagc acctggttct tttcgacggc tgcgcctttc ttcacggaaa
    10201 cctttttcac tttcacgtcc gcgggagatt tgaggatatt ctccatcttc attgcttcga
    10261 ggacaagtat cgcgtcgcct tttttcactt cctggccttc attcactttt acttcaagca
    10321 cgaggccggg catcggcgct ttcagatcgt tgatcttcgc ccccgacgtg cggtccattc
    10381 ccagcgaatg cagcagctcg tcgtatttgt cttttacctg cagcgggtag ctgcggccgt
    10441 tcaccttcac ggttaccgtt ttcgacgcga catcggcttt ctcgatctcg acttcgtacg
    10501 aacggttatc gcggataacg tggaatcgtc cgtggccgga agagatgaca tcgcatgaaa
    10561 atttctttcc gtcgatcgag ccggtccatt ttcctgcttc atccaggaca agcttgtgct
    10621 gcttgtttcc ggcggtaact gtaaaagatg atttttccat ttatgcgtgg gcgtcaggca
    10681 aagttagggg gaatttttac ttggggccct ttagaggtgc atgaattcgt tcgctttccg
    10741 gtgaatgcat ttccggcccg cctttattct tcgcgggatg aaagcgaatt cattcttcaa
    10801 cagcgcggga acgaaaaccg gcccgccgtg ttttcattga tgaaagcagc ggaattcctg
    10861 tttaacggcg caacggtcga aaacaggtac ggaaggattt gtgttaacat aaaatttggc
    10921 caactttggc ctcgctcctt taacaggtaa aaaaattttc cgcgatgtat aaatacatct
    10981 tcccggccac gctcctcgtt tttctctttg cgtgcggcca ccagcagcaa accaccgcct
    11041 cgaaacccgg tccgcgtaac ccgcacatgc aggcgacaga caaaccggtg atcgatccga
    11101 acctgaacgc agaatcgcga agcgattaca atccttcgtt cacgcgcgtg agcgacctgc
    11161 tgcacacgaa gctcgacgtc agcttcgact ggaacaagcg ctacatgtac ggcaaagcga
    11221 cgatcaccgc gcgtccgtat ttctatcctt cttccacgct cgagctggat gcgcgcggca
    11281 tggatatcaa acgcgtggcc atggtcaacc gcgggatttc cgatgcgggc atggtgaccg
    11341 acgatacgac ggcgctcaag tatacgtatg atcagaaaaa gctgaagatc gatctcggcc
    11401 gcgtgttttc gaaagagcag gagtacaccg tcttcatcga ttacatttcc aaacccgacg
    11461 agctgaaaga tctcggcggc agcgcagcga tctcggaaga caagggcctt tatttcatca
    11521 accccgacgg atcgaacccg aactcgccga aacagatctg gacgcagggc gaaacgcaat
    11581 ccaattgcgt gtggatgccg acgatcgacc gtccgaacga acgcatgacg gacgagatct
    11641 acatgcgcgt tgaagacaaa tacaaaacgc tttcgaacgg agttcttgcg gaatcgaagg
    11701 agaacggcga cggaacacgc acggaccatt ggcggatgga ccttccgcat gcgccgtacc
    11761 tggtgatgat ggccgtcggt gatttcgcga tcgtgaagga caaatggcac gacaaggaag
    11821 taagctatta cgtggagccg cagttcgagc cgtatgcgcg ccagaccttc gggaaaacgc
    11881 cggacatgat cgacttcttt tcgaaaaagc tcggcgtgga ttacgtgtgg ccgaaatatt
    11941 cacaggtgtg cgcacgcgat tacgtttcgg gcgcgatgga aaatacatcg gcaacgctgc
    12001 acggtgattt cgtgcagcgc gatgaccgcg aatacctcga ccgtacgtat gaagattata
    12061 tttcgcatga gctgttccac cagtggttcg gtgatcttgt cacctgcgaa tcgtggtcga
    12121 acctcccgct caacgaatcg ttcgccacgt acggagaata tttgtgggac gagcatgaat
    12181 atggaaaaga gacagcagat tacggacatt ataattcgct gcagggctac ctcgacgaag
    12241 ccaatgcagg tcacgccacg ccggatgatc cgggcaagcg cgagccgctc atccggtatt
    12301 attacaccga ccgagaagac atgttcgacg gacattcata caacaaaggc ggccaggtgc
    12361 ttcatctcct tcgccagtac gtaggcgacg atgctttttt cgcgtccctt catctttacc
    12421 tcgagacaaa taaattcaaa gcggccgaag cggacaacct gcggcttgcg ttcgaagaaa
    12481 ccacggggca ggacctgcac tggttcttca acgaatggtt ttattcaccg ggtcaccccg
    12541 agctggacat ttcctacaat tacgatgcag cgaaccagaa agaacgcgtg atcatcaaac
    12601 agacgcagga ccgttccaac ggaacgcccg tgttccgcat cccgctgagc gtggatgtgt
    12661 acaacgggga taaagtgaca cgcgaggaat gcatcgtaaa atcgacgtgc gacacgttct
    12721 ggtttgcttc cccggcgaaa cctgatttcg tcaatgtcga tgcgcagaaa acacttccct
    12781 gtttcaagat cgatcatcac acggcgcagg aatgggcatt tctctataac cattcgaagc
    12841 tgtacgtcga ccgcgtggaa gcaatcgaca accttgcgcc cgaaggcctc ggcggagaag
    12901 tgacgatgat gatgaacaac aagccggtca catcacgaca cggtgcgatg gcaggtccgg
    12961 attccgcaat tgctgttcag acggtgacga aagcgctcga cgatgagttc tgggcgatac
    13021 gccagatggc gtgcgaggag ctcggcgatt atgccgacca tgtgaaggat aagctcaccg
    13081 cgctcgcgaa aaaagacgag aaatcgatgg tgcgtgcagc ggcaatctcg gcgctcaatg
    13141 cgggcagcaa aagcgacgac ctgaaaagcg tttacctcga tgcgctcaac gaccgttcct
    13201 ataccgtgat cacgcgtgcg ctcgttgcgc tggccgatca tttccccgat accgggatgg
    13261 acgaagcgaa aaaacgcgag aacgatcccg cgcgttcgat gaagatcgct gtggccaatg
    13321 cgtattcgca ttgcggaacg gaagcgcagc agccctggtt cgagaaaaca atgaatacgc
    13381 tgtacggtgt gacgcagcgc gaattctgtt acgtctacac ggctttcctg gtcaggaaat
    13441 gttcgcctgc cgctactgaa aaagcgatcc cggcggtgca cgcgctttat acggacaacc
    13501 attcgcccac tgcgatccag ggagcgcaga cgatcatttt ctcgctcgat tacgtttacg
    13561 cgaaacgcct ggacaacctg cagaaaaaga tcaacagcac gaaaaagaaa tcgccggcgt
    13621 atgcaaaaat gctggctgac aaggcagaca tgcagcatct gcttggtgtg ctggacgaag
    13681 agaagaagaa aatgaccgcg gacaaataag ccggaaggaa tttattccgg caattcctcg
    13741 taaccgtgat cgacgtggag gtgtgatccc agcgcggctg caacggcgag ctcgtcgcag
    13801 cgttcgttga acgggttgtc ggcatgtcct ttcacccaga cgaacttcac gtgatgcttc
    13861 gggaatattt tcaggaaacg catccacagg tcagcgtttt ttttcccttt gaatcctttc
    13921 tgctgccagc cgaacaccca ttttttttcc acgctgtcga cgacgtatct ggaatctgaa
    13981 tagatcgtca cgtggcttcc cggaactttt aatgattcaa gcgcgacgat cacgctgagc
    14041 agctccatgc ggttgttcgt cgtggtccgg aaaccctgcg acaattcttt ccggtgattc
    14101 ccggacatga gaattacccc gtatccgccc gggcccgggt tcccgcgtga agcgccgtcg
    14161 gtataaactg tgatgttgtg cgacataaat tccgcggtga agatagtggt cgtggccgga
    14221 tttgccggag tcattttgtt tgtgcgtccc ttttttctaa tttgttgttg gcaaacaatt
    14281 ttaacaaccc ttaatccccg cacatgaaac aggtatacac ccatgttttc ctggttttgc
    14341 tgtctgcatt tctcatagga gaaaaagcaa atgcacaggt gaacgttacg atcacgtcct
    14401 ttaatgatgt gctttgtaac ggaggcaacg atggttccgc gacagcgtcg gcttccggcg
    14461 gaacagggcc gtattcgtat tcgtgggcgc cttccggcgg aaccaacgca acggcgggcg
    14521 ggcttgttgc gggagtctat acggtgactg ccaccgacgc tacgctcgcc agcgcaacgg
    14581 caaccattgc catcacgcaa ccgacgctgc tttcgatctc cacgtccagc gtacccgcca
    14641 cgtgcgggtt cccgaatggc agcgcgagcg gatctccctc cgggggaact ccgccgtata
    14701 cttattcatg ggcgccttcc ggcgcaacca gcgcaacagc gaccaacctt gcctcgggct
    14761 cttacacgat aaccgtaacc gattcgcatg gctgtgtcgt gaccggcgta gtcgcagtgc
    14821 cgaattaccc ggcgccgatt tcaaatgccg gacctgatca gaacatttgt cccgggggaa
    14881 caacggtact ttccgggagc ctcagcggcg gatcaccgcc ctatacctat tcctggtccg
    14941 ggccttccgg ttttttttcg aacctgcttt ctccgtcggt ctcaccggcc acaacctcca
    15001 cgtatacgtt taccgtgacc gacagcaatg gatgctcttc gtcagatgtg gtaacggttt
    15061 tcattgcgcc cctgctgaat ctcactttta attcctcccc ggccacgtgc agccagacca
    15121 acggcatgat cagcgttaca ccttcaggag gcgtttctcc gtatactttt ttatggggga
    15181 ataacagcac gaacgatacg cttttcaatg tatccgcagg attgtattcc gtaacagtaa
    15241 cggatgccgg cggatgctcg aattccgggc agggcgccgt gagcaatgcc accgggcctg
    15301 tcattacgct gagcggtgta accaatcctt cctgctacgg ttccacgaac ggatcggcga
    15361 cagcgaacgt aaccggcaac gcaccaccgt attcttattc ctggaacacg cagcccgtgc
    15421 agacgacgcc gacggcgggc gcacttccct ccgggactta tttttgcacg gtgaccgaca
    15481 cgaacaattg ccagtcttcc gtgtccgtca cactggtgca gccaccgcag ctttatctcg
    15541 gcgtacagca actggcgcct gcaaacggga attcatccgg ttcggcgaat gcgtgggccg
    15601 gcggcggaaa tccgccctat acgtttgcat ggtcgaacag catgagcggg gacaccatta
    15661 ccggtcttac gagcggcgcg tattccgtta ctgtgaccga caattccggc tgcacgtcgc
    15721 agggttctgt tttcgttgcg aatatcaacg cgacgatcgt aaccggaaga atatatgccg
    15781 acattaattc gaactgcgtt tttgacgccg gtgacatgcc gatcgccaat cagtatgtcc
    15841 ctttttatcc tgcgcaggtg tatgcgatgt cagactcttc cgggatgtac acagcttatg
    15901 ttaccacgac cggtacacag accgaatatt cgaacttcaa ctggatgtcg ccgtacacaa
    15961 caggcttttg ccccgcgacg agcacgcgtg tcatcagcat tcctgcattg tatgatacca
    16021 ttcccgggat ggatttcgcc cgccaggatt ctgcaaacgt ccaggacctc cgcgtttcgc
    16081 tttccttcta ccaggcaccg cgtgcaggat ttttgcagac tgccgcgatc tattaccaga
    16141 acgtgggaac ggttcccgtt tccaatacga cggtgaactt tgttttcgat tccattctca
    16201 cctatgtatc ttcttccgcg ccgccctccg tcaaccagcc ggcttacctg gaatggaata
    16261 caggaacgtt gcagcccgga gaatctcaca tgctcctcgc cacgttgcag gttcccacga
    16321 tacagaacgg aggatggatc gggcgaccgc tgttctattc ggccgcgatc aatcccaacg
    16381 ccagtgacgt tacgcccgta gacaacggag acgacgcgtt cagtcacatc atcgcatcgt
    16441 atgatccgaa tgaaaaaaca tgttatgctg caggaatgga ttcgctcggg aatatccttc
    16501 cttcggattc cgttctttcg tacacgatcc atttccagaa tacaggaacg gatacggcgt
    16561 tcaacattta tgttatcgat acgctttccc cgtatctcga tccggtaacg gtgattcccg
    16621 gagcgtcttc gcatccgtat tcgttcgaaa tgtatggcaa cggcgtcatg cgttttgatt
    16681 tttacaacat tcttcttccc gacagcaacc atgattccga aggaagcaac ggttttgtca
    16741 aataccgggt gaaggtcagg cccaacacgg cgcccggcag cacgatcgac aacaccgggt
    16801 acatttattt cgatttcaac ccggcggtcg ttacgaacac tacgtcgaac accatctgga
    16861 ataatgtccc gacggcggtg catgaaaatt ccacatccgg catctcggcg ttcccgaacc
    16921 cgttcgatga atcgacaacg atcgttcttc cgcagcaata tgccggcaag caaaaccagc
    16981 ttgtcattac cgatgcagcc ggaagagtgg tgcgcagtga aagttttacc accagctcgt
    17041 acacgttcgc gcggaataca ctcgacagcg gcatgtattt cctcaccgtt tattccgatg
    17101 gaaagaaaac gggtgtcacg aagattgttg ttcaataaaa aagccgggaa aaaattctga
    17161 ccctcttttc cgttctgctg acagtaaaag acgcactttc caatactacg gttactgctg
    17221 ttgcgtccgt aattcgacct gtcgcgatag cggttaacgt acccggactg tcgccgggaa
    17281 atagatcatt cccggatagc gcgcctgttt cttcagcaag cgtgaagagc cgttttaaac
    17341 gacctatctt tcggatatgt tcggaattat aggaggacct ggctggctgt ttgtacttct
    17401 tgtcctgatc gacatcgggt ttgttttcgc gatgactatt ctgtggctgc gctccatgaa
    17461 caacacgatg gatgcgtgcc ctgtttattc gcggacggcg gaacccgcgc tggtctggct
    17521 cacgctgatc ccgttgttcg ggtttgtgtg gcaattcatt gccgtgttca acgtttcgaa
    17581 ttcacttgcg aaagaatatg cgcggcggaa gtggtcgagc gaagagaacc gccccgcgct
    17641 ggaaacaggg atcattgcct gcatcgccgt cgtggtcgtt acattcgtac gtctcttttt
    17701 tcccattcac ccggggatcg gtttcatcgg ctcgatggtc gtcgcgttct gcatgtacag
    17761 gcacatggac cgcatgcagg cgtaccgcga gcgtctggaa aaagagcggg atccgacgct
    17821 tcagttcggg cagatcacac cgaagaagga agttccgaat ccttttgcgg atctttaccc
    17881 gaaagccggc gtgagctatc cgccggcagc tattgttcat catcagcaaa agaacaccgc
    17941 gtcatccatg aagaaccgct ccacgccgct gcccggaaat acaagcagct atggcgaggt
    18001 tccgccggaa tattctccct ggatgccgaa gccggataaa aaagatgaaa aataaggaag
    18061 cgcggtttca gcgccgggag aagtaatttt acagcgcgat cagctacgtt cagatgtcag
    18121 tgctcagctt ttacgggatc atgtggctcg tcctttttgc gggatgggcg ggcactacgg
    18181 catggtcgaa cgtggtctgc atccgcgccc tctcgctgac gcagccgcct gcgcggagaa
    18241 tggaaccgca gaatgtgtgg ctgacgttcg tcccgctgtt caatctctac tggcagttcg
    18301 tggtgacgta cgcggtggcg gacagcctcg gtgatgagat gcggcgcagg aactatattc
    18361 cacgccaggc gcgccccgga ctttcacagg gaatgacggc atgtattttc ctttgtttcg
    18421 cggtggttcc cgccatgggt ttgcttattg ccaacatcag caacattgtc cgcattatcc
    18481 acctgcagac tgtcagccgt ttttcagacg agctggaaga actgatccgc gtccaaaatg
    18541 cctatgcgcc ggctgttccg caggagatga tattacaaca gcaggtaaac ccggaggaag
    18601 cggagaaaaa agaaaaagaa aaccccgaac ggttcatgcc gcctccgacg ccggaagatg
    18661 aatggaaaag gtgggggaga aaagattaaa aaacggaaag attgtcaacg ggaaagggca
    18721 gaacatgaaa gacattgaaa acaggaatga cgttgagcag cttgtcgggc tgttttataa
    18781 aaaagcgatc cacgatactg tcatcgggca ttttttcacc gcggtaattc ctatttcctg
    18841 ggaaaaacac attccgctca tcgtttcatt ctgggaatcc attcttttcg ggaatgcatc
    18901 gtaccgcggc aacccgatgg aaaagcacat tgagctcagc aggctttcaa aaatggaagc
    18961 gcatcatttc gaacgctggc tgaagttgtg gaaagaaaca acggatgaaa atttcgccgg
    19021 gccgaaagcg aacgaagcga aggtaagggc ggaagcgatc gcgcggctga tgctgcataa
    19081 tgtgcaggca gcacataact gaagatctcc gtgaacttcc gggttgcagt cgttccggtt
    19141 accgcgatca aacaggaatt tcaattgaaa aaggtgatcc aaagccgaac agccccggca
    19201 aaactatttt tctcttccca catggatcag cgcgtcgccc tggttcacca cgggctggtt
    19261 gttcacaccg acgatgaaac cgttggcagg tgcgaagagc tcgtgctcca cttctccgta
    19321 cggatcgcag atgatgccga cgcgctcgcc tttttttact tttccgccgt tcttttttgt
    19381 cgtgtgaaaa agccccgagc tttttgcgcg tatccagctt gattttttca gcagcaccgt
    19441 gcttttctgc gccacgttcg ctttgatcat gccaaaatga ttcatcacgc gcacgcagcc
    19501 gtggatgcct tcccgcacgc tttcgtaatc aaaacgccgc gactcaccac cttcgaacac
    19561 gagtatctgc ttgccgagct tcgccgccgc ctgccgcagc gtattgtcgc ggaaagggga
    19621 attgatgacc atcagcggcg cgaatatttt tccgagccgg agatttttcg gatcgtcaaa
    19681 cacgcaacgg agctgcgggt agttggaaat ttttgcgccg ccggtatgga agtcgaaccc
    19741 gaaatcgatc tgcgggatga tcgtttccat caggtcgtgc gcaatacggc ttccgagcga
    19801 accgttcttc gagcccggga agcagcggtt gaggtcgcgc ccgtcgggaa gatcgcgcga
    19861 gccggcgagg aaggaaataa tattgatcac ggggatcgcc acgatgcagc cgcagcgcgg
    19921 tttctgcaca tcctcgcgct tgatcagctg gcgcaccact tcaatgccgt tgatctcgtc
    19981 gccgtgcata ccggcgagaa aaaggatcgt cggtcccggt tccttcccgt tgaaaatgaa
    20041 aacagggatc tcgatgagcg tgtgcgtcgg tagtttgtaa atgttcagga tgagctgcgc
    20101 gtgctccccc gcttttactg tttgtccctg gatgtggaaa ggttttccca tggtcatttg
    20161 aattttgaat gctgaattca gaattcaaaa ctcaacatta agaattattt gttgcaggcg
    20221 gcagcggata acggagaaaa atgcggccgg gtttcacagg tgtgtatgaa actcattccg
    20281 ctccacgtac tcgatgatct tcccggcgat gtcgacgccg gttgcacctt caatgccttc
    20341 gagcccgggc gatgaattca cttccatcac cagcggaccg cggttgctct gcagcatgtc
    20401 gacgccggcg atcgcgaggc cgagcttctt tacggctttg atcgctgtcg ctttttcctc
    20461 ggcggaaaga gtcaccagcg aagctttccc tccgcggtga aggttcgagc ggaaatcgcc
    20521 ttccgcgccc tggcggcgca tcgcgccgac gatctgcccg tcgacaatga acgcgcggat
    20581 gtcagtgcct ttcgattcgg cgatgaattc ctgcacgaga atgctcactt ccacgtcgag
    20641 gaacgcttcg atcaccgact tcgcggagtt gtgcgtttcc gcgaggatga cgccgatgcc
    20701 ctgcgtgcct tcgagcagct tgatcaccac cggcgttccg cccacctggc ggatgatgtt
    20761 gtcgatgtct ttcgggctcg aagcaaacgc ggttttcggc atgcctaagc ccgctttcgc
    20821 cagcagctgc aggctgcgca gcttgtcgcg tgagcgcacc agcgcctgcg attcgacggt
    20881 ggaaaaaacc cgcatctgct cgaactggcg cacgacggcc gccccgtaga acgtaacgct
    20941 tgcgccgata cgcgggatga tcgcgttcac accagtgagc tcttcgcctt tgtaaaagat
    21001 ccgcgggtgg ttctgctcga tcacgagcac gcatttctgg tgatcgatca cgagcatgtc
    21061 gtgtccgcgt tcgcggccgg cttccacgag gcgattggtt gaataaaggt tggcgttacg
    21121 ggaaaggact cctatccgca tagatcagtg attggtggaa gaaagtgagt tgagcttcgt
    21181 gacgtcgacg acaaaacggt gcttgagcag ccgtctcccg atcagcacgg gatatttcat
    21241 gtttttacga tcggtgagcg acacaatgct tcgtatcgtg cgtttgccga tgcggatgcg
    21301 tgtgcgaacg atgtaacgca tttcctgttc cccgaaagag ctgcggatga gcttctgctc
    21361 gaatttctga aacacgtgct cgcggtcctg gtattcggga tgcgattcat ccagcaggcg
    21421 gaagtgcagc acgccgtttt cgaggcgtac atgatggcaa tgaagcgctg tcgtatttgc
    21481 gccggtgtcg atcttcgcgg taaggccgaa caggccgagg tcggggaagt ccaccacctc
    21541 tcttctgccc accgtttcca ccggtcgtat tttcgcgcgc tttttcaaac ggcagtgcgt
    21601 gatttatttt ttgtcgcgga gatccatctg cccgatggcc atcatctggc gcatggtcct
    21661 ttccatgcct tcgttggagc cgtcgaggaa gatcacgttg ccttttccgt gctctgcgaa
    21721 attcttgatg gcttccgtcc acatgctgaa caggatcagc gatgcgtcga ggttcgcgtc
    21781 cgtcatcatc ttcgctgcct ggctcatacc ttttgcaact tcttcgcgga aaagggcgac
    21841 gccctgtcca cggagctgcg ctgcgtcttt ctccgcctgc gccgcgatct tgatcgcatt
    21901 accttccgct tctgcagctt ttgtcttggt gatgaggagc gcctggcctt cgttctcggc
    21961 ggcggctttc aggttgttcg aggcaacgac tttcgccatg gagcgcatga cttcgtcatc
    22021 gaacgtgatg tcgttcagct gaagatcgat gaggtgatag ccccagccct cgagcgtctg
    22081 gtcgagctgt tcctttaccg cttccacgat atcgcggcga agcgaaagaa cttcagattg
    22141 ttttttcgtt gcgacgaacg cacgtacgga accttcgatg gagcggacga gcgcctgcat
    22201 aaggttctga tcgttaacga atttgaacgc cacgttctgg atcgtctgct catcctggtt
    22261 cacaactgca tacagcagca tggcggtgaa gttgacgttc gcctggtcga tcgttacggc
    22321 ctggaagccg agctccgccg aacggttctg gatggaaatg cgtttgtaaa cacgctctac
    22381 gaacggaact ttgaaattca gcccgggacg cagcacgcgg ttgtattttc cgaaaatcgt
    22441 ggtgacgccg accattccct gctggatggt gacgaaagaa agaaggagta cgaagaagat
    22501 aaagactccg aaaagaatag ctccgatcat gatagtgtgt ttttaggatt tgtgggtttt
    22561 ttgttgggag gagaaaggta ggaaaaaacg ggaaacggca tacgggaaag aaaggccggc
    22621 ggcggcccgg accgaatctt caccgtcaac cccggggctt tatcctctct gtggcctgtt
    22681 tctttcgctg cgaaggtccg gaggcacaaa aattacggat tcattccgca agcaggtgct
    22741 acgctccggc tgaaacttca accccgtatc gtatagctgc ttcaagtatg tcaatgttta
    22801 tgtcgtccag cgttttgaac ctgatgcaat acccggtcac attcgccctg cccagttttt
    22861 ttccatacgt cttggctatg tacgtccggt cttttatacc aagtatatag acggagatcc
    22921 cggttttgtt cccgctaatc ccgatttgaa aaaactccct ggtttttcca ttcgcatatt
    22981 ttattgtttg aaatccgtac ccgatagtag gattgctgac ggttttattc tcgctgttct
    23041 tgccgctgtc gaaccataat ttacattttg gcaacacccg gagcatgagc cggtgcagtt
    23101 cctgcatatc cttgcgtttg ggttcgggct ggctggtaat atactttttg atctgttcct
    23161 gtacgttcat gtaaaatatt gaatcaggtg taacggtttc tggctttgcg ccggggcggc
    23221 acaaaccaat ttaaaacatt tttacaaggc gggacacatg gcaggacccg cctgccgcca
    23281 gctttttttg gaaatacgaa aaaaacatct ttacatttgc ggcatgaaaa tcaatgcgat
    23341 catcaataat atgttctgct acggcaattt ttatacaatt gctgcagggg gccgttgctg
    23401 attttcgctt acaaaatcat accgaaggcc tcccggaaac aggaggcttt tttatttccg
    23461 cactaaaatg agatcatcgc tggtcattaa ttacttcttc aattatatgt tgctgtacgg
    23521 gtgaccagga ctccttttgc attctccaac cctggtcacg acgaccgggg ttttttgttt
    23581 caccaaaaac caaatccaat gaaaacaaga tcgatttatt gcccggtgct caacgaaaac
    23641 gcaacggaag aagccgtcca gttcatcaag ggaaatttca gccggctcct tgccgcccgc
    23701 ctgcacctga agaaagttac agcgcccatt
//
LOCUS       DASTSF010000087         6074 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_166766,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000087 DASTSF010000000
VERSION     DASTSF010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 6074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6074
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166766"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..638
                     /locus_tag="VFU15_02560"
     CDS             <1..638
                     /locus_tag="VFU15_02560"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peroxiredoxin family protein"
                     /protein_id="HEU4716680.1"
                     /translation="VYIFAGMQHPSDWRGWVLPAVPMLFMSFILRGVIPEVLFFQRRS
                     KTKKDSPAETGKPAPDFSLPDIEGKPVKLSDFRGKNHVLLVFVRGDWCPTCHIMIRMY
                     EQNREKFLAKDIVTIGISPESTETNRAMMERLGWKNMLLADTKKEVAYKYGILFRENE
                     KEKEAETNDGHALPASFLVDRSGVMRYMSRSDRAGEFLSPALIFPVVENLS"
     gene            677..1570
                     /locus_tag="VFU15_02565"
     CDS             677..1570
                     /locus_tag="VFU15_02565"
                     /inference="COORDINATES: protein motif:HMM:NF017932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent peptidase"
                     /protein_id="HEU4716681.1"
                     /translation="MTLTDILLTEKLSGPEVLLVLLGIGGGAIGLMYVIGWGLKKTAE
                     AVSDVADSKAVLDLRYGKEFRDKVLTLLDDFVYFRRLSPGGKEKFIDRVVYHYSKKEF
                     EGENGYEPDEKIRIHICATAAQLTFGMSKDWLTGIETIRLYPENFRLYAGGPLMKGAT
                     SPSGTLFISVKDYEEGYRNGSDNFNVGLHEFGHQLFIEFLTAAKEDDAVKQVLFPYDA
                     EAEKELAKKDRTEVFLRKYAFTNRHEFFAVSIETFFESPVDFSHRLPELYRILSQMLN
                     QDPRNSAADYAVQAETGDMGI"
     gene            complement(1619..2095)
                     /locus_tag="VFU15_02570"
     CDS             complement(1619..2095)
                     /locus_tag="VFU15_02570"
                     /inference="COORDINATES: protein motif:HMM:NF015147.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LEA type 2 family protein"
                     /protein_id="HEU4716682.1"
                     /translation="MKRFRFCVFLFPLFFLSSCKVEPVVPKSVENVKFDRIDPLKGIV
                     VLDMGLRINNPNKFSITVYHLDVDITVGGVPLGKVSMDDKFKIRKDTEEVYPVKVNAQ
                     IRDIITGIPKLLAAVQKKQTDVTAKGSIKVGSGIFRKIFPFDIDQKKVPAEDANKK"
     gene            complement(2246..3634)
                     /locus_tag="VFU15_02575"
     CDS             complement(2246..3634)
                     /locus_tag="VFU15_02575"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEU4716683.1"
                     /translation="MSAQKQFISGNKDRFINELFELLRIPSVSADPRHKDDMIRTAEA
                     VKAKMEAAGATNCEICPTGGHPVVYGEKIIDPKLKTVLVYGHYDVQPPDPLDLWTTPP
                     FEPVIRKTDIHPEGAIFARGSCDDKGQMFMHIKALEVMLKTDSLPCNVKFMIEGEEEV
                     GSANLASFLEKNKAKLKADVILISDTSILANDTPSIDVGLRGLAYMEVEVTGPNRDLH
                     SGVYGGAVANPVNVLCRMIASMHDENFHITIPGFYDDVMTLSDAERADLNRAPFNLDE
                     YKKDLGIGDVQGEKGYTTLERTGIRPTLDVNGIWGGYTGEGAKTVLPSKAYAKISMRL
                     VPNQTSDKIAAMFEKHFKSIASGSVKVKVTRHHGGEPVVVSTSSDAYLAAAKAMEETF
                     GKKPIPTRGGGSIPIVALFKSVLGLDSVLFGFGLDSDALHSPNEHYGVFNYMKGIETI
                     PLFYKNYANGKN"
     gene            3802..5706
                     /locus_tag="VFU15_02580"
     CDS             3802..5706
                     /locus_tag="VFU15_02580"
                     /inference="COORDINATES: protein motif:HMM:NF038133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor L domain-containing protein"
                     /protein_id="HEU4716684.1"
                     /translation="MRTSLPKFTFLLAIVSFFAGTSALRSQIVTTNTLTPTQLVQNVL
                     LGPGIQAFNITYTGYANAIGKFVGGNSGTPGLGIDSGVVMTNGTILANDPTYGSGNGP
                     QGPNNSSGAGVDNGQPGDTYLDGVAGCQTFNACVLEFDFIPMADSVKFNYVFGTDEYM
                     EYVSGGFADVFAFVLSGVTVPLAPTNIALIPSTTTPVTALNVNANVNSQYYVDNENPP
                     GTTVQYDGFTTVLQAKYPVQCGETYHIKLACADALDGIVDGGVFLQAGSFTTGTVNLS
                     TQISYGSTNDSTLYEGCGQACLILSRTGSTSQPDTVDLAIGGTATNGVDIIPQIPNQV
                     IFQPGQDSIVLCVQAQQDGIPEGLETFTLTSNVSGPCVQSVTSLTLYLSDAYNITANA
                     GNDTSLCSSSAVTLYCNATGGIMPYTYSWNTGATTPDITVSPTTTTSYVVTVSDQCGT
                     PVGVDTVNVFLPNTAPFTLTTSPDLLLCNGDAALMTASPSGGSQPYTITWGTLAGADS
                     VPNPSGTINQFTPGGNGTFVVSARDGCGNIANDTITVTLQECAIVPPNVFTPNGDGTN
                     DNLVFHGLDKFPGSSLYVYDRWGTKVYESSDYQNDWNGGGLHDGTYFYILNVAGDKSL
                     TGFVTLLSQK"
     gene            5767..>6074
                     /locus_tag="VFU15_02585"
     CDS             5767..>6074
                     /locus_tag="VFU15_02585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716685.1"
                     /translation="MLRRIYILVFFLVFFANDINATHIVGGEIFYDCLGGNNYRITLK
                     VYRDCLNGQAPYDNPASVGIFNSFGVLIDTLSMNFPGSAQVPPTINSPCYSPPGNVCV
                     "
BASE COUNT         1383 a         1704 c         1603 g         1384 t
ORIGIN      
        1 ccgtctacat tttcgccggg atgcagcatc cttccgactg gcggggctgg gtccttcccg
       61 cagttcccat gctgttcatg tcgttcatcc tccgcggcgt aattcccgaa gtgctgtttt
      121 tccagcgcag gtcaaaaacg aaaaaagatt ccccggcaga aacgggaaaa cccgcccctg
      181 atttttcatt gccggacatc gaaggaaagc cggtaaagct ttctgatttc cgcggcaaaa
      241 atcacgtgct gctcgtgttc gtgcgcggcg actggtgtcc cacctgccac atcatgatcc
      301 gcatgtacga gcagaaccgt gaaaaattcc tcgcgaagga catcgtcacc atcggcatca
      361 gcccggaaag cacggaaacg aaccgcgcga tgatggagcg cctcgggtgg aagaacatgc
      421 tgctcgcgga tacgaaaaaa gaagtcgcgt acaaatacgg catcctgttc cgcgaaaatg
      481 aaaaggagaa agaagcggaa accaatgacg gccatgcgct gcctgcgtca ttcctcgtcg
      541 accgcagcgg cgtgatgcgt tacatgtcgc gttccgaccg tgccggagaa ttcctcagcc
      601 cggcgctcat cttccccgtc gtggaaaatc tcagctgacg cgccgcaacg atttcatcaa
      661 cctgcatttg cattccatga ccctaaccga catcctgcta acggaaaagc tgagcggccc
      721 cgaggtcctg cttgtcctgc tgggaattgg aggaggcgcg atcggcctca tgtatgtcat
      781 cgggtgggga ctgaaaaaaa cggcagaagc cgtgagcgac gttgcggaca gcaaagcggt
      841 gctcgatctc cgttacggga aggaattccg cgacaaagtt ttgaccctac tcgacgattt
      901 tgtttatttc cgcaggctct ccccgggcgg aaaagaaaaa ttcatcgacc gtgtcgttta
      961 tcattattca aaaaaagagt tcgaaggaga gaacggttat gaacccgacg aaaagatccg
     1021 catacacatt tgtgcaaccg cggcgcagct gacgttcggc atgagcaaag actggctgac
     1081 gggcatcgaa acgatacggc tgtaccctga aaattttcgc ctgtatgcgg gaggtccgct
     1141 gatgaaaggc gccacatctc cttccggaac gcttttcatt tccgtgaaag attacgaaga
     1201 aggatacagg aacggaagcg ataatttcaa tgtcgggctg cacgaattcg ggcaccagct
     1261 ttttatcgag ttcctgaccg ccgcgaagga agatgatgcc gtaaagcagg tactgttccc
     1321 gtacgatgcc gaggcggaaa aagagctggc gaaaaaagac cgcacggaag ttttcctgcg
     1381 taaatacgcg ttcacgaaca ggcatgaatt ttttgcggtt tcgatcgaaa cgtttttcga
     1441 atcgccggtg gatttcagcc accgcctgcc ggaactttac cgcatccttt cacaaatgct
     1501 caaccaggat ccgcgcaaca gcgcggcaga ttatgcggtg caggcggaaa ccggcgatat
     1561 gggcatctga cgtttccatt cgaaagcagg aaacaggaaa acggcggccg ggaacttttc
     1621 acttcttgtt cgcatcctcc gccggaactt ttttctgatc gatatcgaac gggaatattt
     1681 tccggaaaat gcccgatccc actttgatcg atcctttcgc cgtcacgtcc gtttgctttt
     1741 tctggaccgc agccagcagt ttgggaatgc cggtgataat atccctgatc tgcgcgttca
     1801 ctttcacagg atacacttct tccgtgtcct tcctgatttt gaatttgtcg tccatactta
     1861 cttttcccag cggaacaccg cccaccgtga tgtcgacgtc gaggtgataa acggtgatgg
     1921 aaaatttatt cgggttgttg atgcgcagtc ccatgtcgag aacgacaatt cccttcagcg
     1981 gatcgatccg gtcgaacttc acattctcca cgcttttcgg gacgacgggt tcaaccttgc
     2041 acgaggaaag aaagaacagc ggaaaaagaa aaacgcagaa gcggaaacgt ttcatgaagc
     2101 gaagtgtttt gttaatgaat gcgaatgaaa tggactgtgc tgatctccgc aaacatcaaa
     2161 ccgtcatccg gcggcaattc acaattaaat ttaaaaccgg cgatcgcagg taaatgcaac
     2221 cgccggcttt tcatcttttc ccggttcagt ttttcccgtt cgcgtaattc ttgtagaaca
     2281 gcgggatcgt ttcaattcct ttcatgtaat tgaacacgcc gtaatgttcg ttcggcgaat
     2341 gcagcgcatc ggaatcgagg ccgaagccga aaagcaccga gtcgagaccg agcacgctct
     2401 tgaacagcgc aacgatggga atggacccgc ccccgcgtgt agggatcggt tttttcccga
     2461 acgtttcttc catcgctttt gccgcagcga gataggcatc ggaagaggtc gacaccacca
     2521 ccggctctcc gccgtgatga cgcgtcactt tcaccttcac acttcccgac gcaatgcttt
     2581 tgaaatgttt ttcaaacatc gcggcgatct tgtcggacgt ctggttcggg acaaggcgca
     2641 tggaaatttt tgcgtacgct ttcgacggca gcacggtttt cgcgccttcg cccgtatagc
     2701 cgccccagat gccgttcaca tcgagcgtcg ggcggatgcc ggtgcgctcg agcgtggtgt
     2761 atcctttctc accctggacg tcgccgatgc cgagatcttt tttgtattca tcgaggttga
     2821 acggggcgcg gttgagatcg gcacgttccg catcggaaag cgtcatcacg tcgtcgtaaa
     2881 aaccaggtat ggtgatgtgg aaattttcat cgtgcatcga ggcgatcatg cggcagagca
     2941 cgttcaccgg gttggccaca gcgcccccgt aaacaccgga atgcaggtcg cggttgggcc
     3001 cggtcacttc cacttccatg taggcgagcc cgcgaagtcc cacatcgatc gacggcgtat
     3061 cgttggcgag aatggacgta tcggaaatga ggataacgtc cgctttcagc ttcgctttgt
     3121 ttttttcgag gaatgatgca aggttcgccg agccgacttc ctcttcacct tcgatcatga
     3181 atttgacgtt gcacggaagc gaatccgttt tcagcatcac ttcgagcgcc ttgatgtgca
     3241 tgaacatctg gcctttgtcg tcgcaggagc cgcgtgcaaa aatggcgcct tcgggatgga
     3301 tgtctgtttt gcggatcacg ggttcgaacg gcggagttgt ccagaggtcc agcggatcgg
     3361 gcggctgcac gtcgtaatgc ccgtacacga gcacggtctt cagcttcggg tcaatgatct
     3421 tttcaccgta aaccacgggg tggccgccgg tcgggcagat ctcgcagttc gtcgcgcctg
     3481 ccgcttccat tttcgctttc accgcttccg ccgtacggat catatcgtct ttatgcctgg
     3541 ggtccgcgct gacagaagga atgcgcagga gctcgaaaag ttcgttgatg aagcggtctt
     3601 tattcccgct gatgaattgt ttttgtgctg acatgatgaa atcgattttt tatttgagaa
     3661 gaggtacgaa tttgcaataa aaagacgtgc aaaaagaatt caccggttga tttctgcgca
     3721 tttggctttc agcttgaaaa tcaagcacaa aaggtgtatt tttgtttttc ggcttcacgt
     3781 gtaatttcta atttctacaa catgcgtacg tctttaccca aattcacgtt cttgctcgcg
     3841 atcgtttctt tcttcgccgg aacgtccgct ctccgctcac agatcgtcac caccaataca
     3901 ctcaccccga cgcagctcgt gcagaacgta ctgctgggcc cgggcatcca ggcgttcaac
     3961 atcacctata ccggttatgc gaacgcgata gggaaatttg tcggcgggaa cagcggaacg
     4021 cccggactcg gcatcgacag cggcgtagtg atgaccaacg gaacaatcct tgcgaacgac
     4081 cccacgtatg gttcgggcaa tggcccgcag ggaccgaaca attcatccgg cgccggcgtt
     4141 gacaacggcc agccgggaga cacctacctc gacggcgtcg cgggctgcca gaccttcaac
     4201 gcctgcgtgc tcgaattcga ttttatcccg atggccgact cggtgaaatt caattacgtt
     4261 ttcggaacgg atgaatacat ggaatacgtt tccggcggct tcgctgacgt attcgctttc
     4321 gtgctcagcg gcgtaacggt cccgctggcg ccaacgaaca tcgcgcttat cccgagcacc
     4381 accacaccgg tgacggcact taacgtcaac gcaaacgtga attcgcaata ttacgtcgac
     4441 aatgaaaatc cgccgggaac caccgtgcag tacgacgggt tcacaaccgt tctgcaggcg
     4501 aaatatcccg tgcagtgcgg cgaaacgtat cacatcaagc tggcctgcgc cgacgcgctc
     4561 gacggcatcg tcgacggggg cgttttcctg caggcaggtt cattcaccac ggggaccgtc
     4621 aacctttcca cgcagatcag ctacggaagc acgaacgatt ccacgcttta cgaaggatgc
     4681 ggacaggcct gtctcatcct ttcacgcacc gggagcacat cacagcccga cacggtcgac
     4741 ctggccatcg gcggtacagc gaccaacggc gttgatatca ttccgcagat cccgaaccag
     4801 gttatcttcc agcccggcca ggattccatt gttctgtgcg tgcaggcaca gcaggacgga
     4861 attcccgagg gactggaaac attcacgctc acgtcgaacg tcagcggccc ctgcgtgcag
     4921 agcgtgacga gtctcacgct gtacctgagc gacgcgtaca acattaccgc aaacgcaggc
     4981 aacgatacat ccctgtgcag ctcttcggcc gttacgttgt attgtaatgc aaccggcggg
     5041 atcatgccat acacctattc gtggaatacc ggtgcaacaa cgcctgatat caccgtttcc
     5101 ccgacgacga cgacgagcta tgtcgttacc gtgagcgacc agtgcggcac gcctgtgggc
     5161 gttgatactg ttaacgtttt cctgccgaac acggccccgt tcacgctcac cacttcgccc
     5221 gatctcctgc tctgcaacgg cgatgcggcg ttgatgacgg ccagtccttc cggggggtcg
     5281 cagccttaca ccatcacctg gggaacgttg gccggtgcgg attccgttcc gaacccgtcg
     5341 ggaacgatca accagttcac cccgggcgga aacggcacgt ttgttgtttc agcacgcgac
     5401 ggttgcggca acatcgccaa tgatacgatt acagtcacct tgcaggaatg cgcgattgtt
     5461 ccgccgaacg ttttcacccc gaacggcgac ggcacgaacg acaacctcgt tttccacgga
     5521 ctggataaat tccccggcag ctcgctgtac gtttatgacc gctggggaac aaaagtttac
     5581 gagagctccg attaccagaa cgactggaac gggggcgggc tccacgacgg aacctacttc
     5641 tatatcctga acgttgcagg cgacaagagc ctcaccggat ttgtgacgtt gctgtcacag
     5701 aaataaacac gcagtcgttg cctgaactcc ccgaggaacg cctggaaaaa ggttattacc
     5761 cggcctatgc tgcggcgtat ttacatattg gttttctttc ttgtcttctt cgcgaatgac
     5821 attaatgcca cgcatatagt cggcggggag atcttttacg attgtctcgg cggcaacaac
     5881 tatcgcatta cactgaaagt ttaccgcgac tgtctcaacg ggcaggcgcc ctacgacaac
     5941 ccggcttccg tcgggatctt caacagcttc ggtgtgctca tcgatacgct ttccatgaat
     6001 tttcccggat cggcccaggt gccgccgacg atcaacagtc cctgctactc gccaccgggc
     6061 aacgtgtgcg tgga
//
LOCUS       DASTSF010000088         4941 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1729375,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000088 DASTSF010000000
VERSION     DASTSF010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4941
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1729375"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..611)
                     /gene="aroC"
                     /locus_tag="VFU15_02590"
     CDS             complement(<1..611)
                     /gene="aroC"
                     /locus_tag="VFU15_02590"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011709508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEU4716686.1"
                     /translation="MAGNSIGTLFCLTTFGESHGPAIGGVIDGCPPGVVIDTDFVRSE
                     MQRRKPGQSKIVTQRKEEDEVEFLSGIFEGRSTGTPIGFIIRNTDQKSKDYDALKDIY
                     RPSHADFTYDARYGVRDHRGGGRSSARETAARVVAGAIAKLFISRYGISVEGWVSQVG
                     KIVLGKNYTELDLSAAENNIVRCPDEETAREMIALIEQTRKNG"
     gene            720..1430
                     /locus_tag="VFU15_02595"
     CDS             720..1430
                     /locus_tag="VFU15_02595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716687.1"
                     /translation="MRCIPFFVLWLTFSLCAQAQDSAQTIPAKKYSVKPAFDFDQRFS
                     FIRDKKVNIWGERGGVLINEKFKVGVGVYFLKDKLKSLAVDTSGPLYTGKRNLYFGTM
                     YFEPFVFRFKFVEFSVPFEAGFGKSIFQVYSSSTNELLNTTKRDFLPTGAGLSLSFKL
                     PPAGRFKPTRWIGINFLAGYRYCVLQNYFQTDYNGMFWSVSGAIFLDRVTDDCREWNH
                     ARKQRKADRPDNSKINAG"
     gene            complement(1431..2246)
                     /locus_tag="VFU15_02600"
     CDS             complement(1431..2246)
                     /locus_tag="VFU15_02600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716688.1"
                     /translation="MKKIFLLLLIFSAAAANAQDSTMAPAHHRRAPRPLRAFLGDTIV
                     QKGMNEIGINIAPVFTTLLGAYPDNEVTWTIYYKRRLQNRLTFRAGLFARNEQYRWPD
                     EGQDNVYYLVTDSTRIMNVLDISPAKRYQLHAGLELRSKAQRRWSGFFAFDVFGGMSF
                     RHVALYDIRQTRESNGSWSESAMIVNGGGSPIYVHATERTWYAGGNPCVGLTYAFNSK
                     WKLLLQTGIYFYFESGDHYTRDEFGQNLINTKRTDFNFDSPGLFNQFEIVYSF"
     gene            2325..3572
                     /gene="hutI"
                     /locus_tag="VFU15_02605"
     CDS             2325..3572
                     /gene="hutI"
                     /locus_tag="VFU15_02605"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020211643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HEU4716689.1"
                     /translation="MRKLIIKNIRGLVNTREPNVEKVSGKEMAELPVTEDAWLAAEDG
                     VIVDYGTMETWPGISDWSGLDVIDANEGYVLPAWCDSHTHLVFAGSREGEFVDRIRGL
                     SYEEIALRGGGILNSAKKLRSTGEDELFESASSRLDEVISLGTGAIEMKSGYGLDMES
                     ELKMLRVIRRLKESASVPVKATFLGAHAIPAEFKNNREGYIRLLIDEMMPAIAAEKLA
                     DYCDVFCERNYFTEEESVRILEAAKKHGMIPKVHANQLSCSGGVQAGVRTGAISVDHL
                     EYIGDGEIAALKNSDTMPVLLPGAAFFLGLPWPPARKMIDAGLPVAVASDFNPGSCPS
                     GNMNQMVSLLCIAHKMTPAEAINAATINGAYAMGISETHGSITAGKKASFIITRPVPS
                     PDYLPYAFGSNLVEKVIIDGKEI"
     gene            complement(3630..4238)
                     /locus_tag="VFU15_02610"
     CDS             complement(3630..4238)
                     /locus_tag="VFU15_02610"
                     /inference="COORDINATES: protein motif:HMM:NF025492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4294 domain-containing protein"
                     /protein_id="HEU4716690.1"
                     /translation="MKNALLSIIAVFGFSGLFSQDLAVGDISQYPKDMVVMTSDQSGD
                     TTVHVMYYTCVIVAEKTFTDPKEKAKWDRLKYDVKVAYPYSVLAKMKLAEMDSQLARI
                     SGEKERKEYNEKCEKELTRQFDSDMRNLTHTQGKILFKLIDRETGSTTYELIKDRRGS
                     FSAFMWQGVAVVFGNNLKDDYDPDGEDANIEQIVQLIELGVI"
     gene            complement(4354..>4941)
                     /locus_tag="VFU15_02615"
     CDS             complement(4354..>4941)
                     /locus_tag="VFU15_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010609382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase"
                     /protein_id="HEU4716691.1"
                     /translation="EMITGIDLVKEQINVAEGNALSFRQEDLKINGHAIEVRVYAEDP
                     ANNFLPDIGKLVTYRRPQGTGIRVDDGFEEGMDIPIYYDPMIAKLIAWGKDRNEAIER
                     MLRAIAEYRITGVETTLSFCRFVLEHEAFRSGNFDTHFIGKYFTPDKLADLFADEYEI
                     AAILGTGFLEEEKSRVSGKTEAPPAISAWKVNRSR"
BASE COUNT         1130 a         1372 c         1282 g         1157 t
ORIGIN      
        1 tcgccgttct ttctcgtctg ctcgatgaga gcgatcattt cacgtgccgt ttcctcgtcg
       61 ggacaacgca cgatgttatt ttctgcagcc gaaagatcga gctccgtgta attttttccg
      121 aggacgatct tccccacctg cgatacccat ccttccacgg agatcccgta acgggaaatg
      181 aacagcttcg caatagctcc ggccaccacg cgcgctgccg tttcccgggc agaagagcgc
      241 ccgccgccgc ggtggtcgcg gacgccgtac ctggcatcgt atgtaaaatc ggcatgggaa
      301 ggacggtaaa tatctttcag cgcatcgtaa tctttcgatt tctgatcggt gttgcggata
      361 atgaaaccga tcggcgtacc ggtcgatctt ccttcgaata tcccggacag gaattccacc
      421 tcatcctctt ctttccgctg cgtgacgatc ttcgactgcc cgggttttct ccgctgcatt
      481 tcggaacgga caaaatccgt gtcgatcaca acacccggcg ggcaaccgtc gatcacgccg
      541 ccgatagccg gcccgtgcga ttcgccaaaa gtcgttaaac agaaaagggt gccaatggaa
      601 ttcccggcca tatcggcaaa aatagctact ttcacgtgtc acgcccccag gcagtttgtg
      661 gcattaaacc aattcttcca gcacttgtca aacggatgat gcaggcgctt ccccgcggaa
      721 tgcgttgtat tccgtttttt gtcctttggc tgactttttc cctgtgcgcg caagcgcagg
      781 attccgcgca aaccattcct gcaaaaaagt attccgtcaa acctgcgttc gatttcgatc
      841 agcgtttttc attcatacgc gataaaaaag tgaacatctg gggcgagcgc ggcggtgttc
      901 tcatcaatga aaagttcaag gtcggtgtcg gcgtttattt cctgaaggac aaactgaaaa
      961 gcctcgcggt ggatacgtcg ggaccgctat ataccggaaa gcgcaatctt tatttcggga
     1021 cgatgtattt cgaaccgttc gttttccgtt tcaagtttgt tgaattcagc gttccgttcg
     1081 aagccggttt cggcaaatcg atcttccagg tgtacagcag ttccaccaat gaattgctca
     1141 acaccacgaa acgcgacttc cttcccaccg gtgcaggact atcgctttcc ttcaagctgc
     1201 cgcccgccgg gaggttcaag ccgacgcgct ggatcggcat taatttcctc gcgggttacc
     1261 gttactgcgt gctgcagaat tatttccaga cggattacaa cggcatgttc tggtcggtga
     1321 gcggcgcgat cttcctggac cgtgtgacgg acgattgccg cgaatggaac catgcgagaa
     1381 aacagcgtaa agccgatcgc cccgataaca gcaagatcaa cgccggctga tcagaacgag
     1441 taaacgatct cgaactggtt gaaaagtccc gggctgtcga agttgaaatc cgtacgcttt
     1501 gtattgataa gattctggcc gaactcgtcg cgcgtgtaat gatcgccgct ctcgaaatag
     1561 aaataaattc ccgtctgcag gagcagtttc cacttgctgt tgaacgcata cgtaagaccc
     1621 acgcagggat tcccgccggc gtaccaggta cgttcagtgg catgcacata aatgggcgat
     1681 ccaccaccgt ttacgatcat cgctgattcg gaccaggaac cgttcgattc gcgcgtttgc
     1741 cggatgtcgt acaacgccac atgccggaag ctcattccgc cgaagacgtc gaacgcgaaa
     1801 aagccggacc atcttctctg cgctttcgat cgcagctcaa gtcccgcatg cagctggtaa
     1861 cgttttgccg ggctgatgtc cagcacattc atgatgcgcg tcgaatcggt tacgagataa
     1921 taaacgttgt cctgtccttc gtccggccac ctgtactgtt cgttccgcgc aaacaggccc
     1981 gcacggaagg tcaaacggtt ttgcagcctg cgcttgtaat aaatcgtcca cgtcacttca
     2041 ttatccgggt aagccccgag aagcgtcgtg aacaccggcg cgatattaat gccgatctca
     2101 ttcattcctt tttgcacgat cgtatcaccg aggaaggcgc gcaggggtct gggcgcgcgg
     2161 cggtgatgcg cgggtgccat cgtgctgtcc tgcgcatttg cggcagcagc tgaaaaaatc
     2221 aaaagcagta aaaaaatctt tttcatgttt tttataagaa gacgatgcag gataattttc
     2281 gttgccatcg tagatttggc aattctatca ttattcatcc gatcatgcgc aaattaatta
     2341 tcaaaaacat acgcggactt gtcaacacgc gcgaaccgaa cgtggaaaaa gtttccggga
     2401 aggaaatggc ggagctgccg gtgacggaag acgcgtggct cgctgccgaa gacggcgtga
     2461 tcgtggatta cggcacgatg gaaacatggc cgggtatatc cgactggtcg ggactcgacg
     2521 tgatcgacgc gaatgaaggc tacgtgctgc cggcctggtg cgattcgcac acgcatctcg
     2581 ttttcgcggg aagccgtgaa ggagaattcg tggaccgcat ccgcggactc agctacgaag
     2641 agatcgcgtt gcgcggcggc ggcatactca attcagcaaa gaagttgcgc agcacgggcg
     2701 aagatgagct gtttgaatct gcttcatcgc gcctggacga agtcatttcg ctcggcaccg
     2761 gcgcgattga aatgaaaagc ggctatgggc tcgatatgga aagcgagctg aagatgctgc
     2821 gcgtgatccg gcggctgaaa gaaagcgctt cggttcccgt gaaagcgacg ttcctcggcg
     2881 cgcacgccat tcccgcagaa ttcaaaaaca acagggaagg ctatatccgc ctgctcatcg
     2941 acgagatgat gccggccatt gctgccgaaa agctcgccga ttactgcgac gtgttctgcg
     3001 aacggaatta tttcacggaa gaagagtccg tgcgcatcct cgaagccgca aaaaagcacg
     3061 gcatgatccc gaaagtgcac gcgaaccagc tgagctgttc gggcggtgtg caggccggtg
     3121 tgcgaacagg cgcgatcagc gtggaccatc tcgaatacat cggtgacggg gaaatcgccg
     3181 cgctgaaaaa ttcggacacg atgcccgtgc ttttgcccgg cgccgccttt ttcctcgggc
     3241 ttccctggcc acctgcacgg aaaatgatcg acgccggact tcccgtcgcc gtggcatccg
     3301 atttcaatcc cggcagctgc ccgtcgggaa acatgaacca gatggtgagc ctgctctgca
     3361 tcgcgcacaa gatgacaccc gcggaagcga tcaacgccgc gacgatcaac ggcgcgtatg
     3421 ccatgggaat ttcagaaaca cacggaagca ttaccgccgg caagaaagcc agcttcatca
     3481 tcaccaggcc tgttccctcc ccggattacc tgccgtatgc cttcggcagc aacctcgtgg
     3541 agaaggtgat cattgacgga aaagagatct gaaaaaaata gaaatcctgg agcttcgccg
     3601 ctccgctttc aaatgttgaa cccggaactt tagatcaccc ccagctcgat cagctgcacg
     3661 atctgctcga tattggcgtc ttcgccgtcg ggatcgtaat catctttgag gttatttccg
     3721 aaaacgaccg ccacgccctg ccacatgaag gcagaaaatg agccacgccg gtctttgatg
     3781 agctcataag tagtggaacc tgtttcacgg tcgataagct tgaagagaat ttttccctgc
     3841 gtatgcgtga ggttgcgcat gtccgagtcg aactgccggg tcagctcttt ctcgcatttt
     3901 tcgttgtact ccttccgctc tttttcaccg ctgatgcggg ccagctgcga atccatttcg
     3961 gccagcttca tttttgcgag cacggaatac gggtaagcca ctttcacgtc gtatttcagg
     4021 cggtcccact ttgctttttc tttcggatcg gtgaatgttt tttccgccac gatcacacag
     4081 gtgtaataca tcacgtgaac cgtggtgtcg cccgactgat cggaagtcat gacgaccatg
     4141 tctttcgggt actgggaaat atcgcccact gccaggtcct gcgaaaacag gccggaaaac
     4201 ccgaaaacag caatgatgga aagaagcgcg tttttcatga cactggtttt agttcaaacg
     4261 atgtgccaat atttggttcc ataacgcctt tatcccgcct ttagttacaa acccggctga
     4321 gaataattgt taacctgcca attgttaaaa tcctcaccgc gagcggttga ccttccaagc
     4381 cgagatggcc ggcggcgcct ctgtcttgcc cgaaaccctg cttttttcct cttcgaggaa
     4441 gcccgtgccc aggatcgcgg caatctcgta ttcgtccgcg aaaagatctg ccagcttgtc
     4501 cggcgtgaaa tatttaccta taaagtgcgt atcgaaatta ccagacctaa atgcctcgtg
     4561 ttcaagcaca aaacgacaaa aagaaagtgt cgtttccacc ccggtgatgc ggtactcggc
     4621 tatggcccgg agcatccgtt cgatcgcctc gttccggtct tttccccagg cgatgagctt
     4681 ggcgatcatc ggatcgtaat agatcgggat gtccatccct tcctcgaacc cgtcgtcaac
     4741 gcggatgcct gttccctgcg gacggcggta cgtaacgagc ttcccgatat ccggtaaaaa
     4801 attgttcgcc gggtcttccg catacacgcg cacttcgata gcatgaccat tgatcttcag
     4861 gtcttcctgg cggaacgaaa gtgcattccc ttccgccacg ttgatctgct ctttcaccag
     4921 gtcgataccg gtgatcattt c
//
LOCUS       DASTSF010000089        14578 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_166087,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000089 DASTSF010000000
VERSION     DASTSF010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 14578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14578
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166087"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(86..649)
                     /locus_tag="VFU15_02620"
     CDS             complement(86..649)
                     /locus_tag="VFU15_02620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716692.1"
                     /translation="MDSGRCNSVLEESCCCFNFNTVFIPSLNPAMKTPFRILLPIILI
                     QFISCGPSAQERAEVEKAKMDSTAHATIDASRRKNDSMVSSQVNVLDSEIVKQQREIY
                     RLENDPDYLYVKTRLATVRARVSTGGSYGWIDKDGKFWEGKDASAAFDAAAKEESDQL
                     KKIDVKNAEIKSLQDQIQNLQALKSDR"
     gene            complement(619..1341)
                     /locus_tag="VFU15_02625"
     CDS             complement(619..1341)
                     /locus_tag="VFU15_02625"
                     /inference="COORDINATES: protein motif:HMM:NF025597.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4339 domain-containing protein"
                     /protein_id="HEU4716693.1"
                     /translation="MKKYYIHDGNNQDGPFSIDELKAKNINKDTPLWYEGLEKWTTAG
                     SVAELNTIFRAPVPPPFEKQNLSTPPPLTKKEPVQSSKAPSQTSPKAKNPTGIILSII
                     GGVLLLILIFSAIQKHIGSDHSSGPNSTNYQETIKSAEEIERENPAQFLDAGGTYNQN
                     FWGDAMKIHGNVKNKATVANYKDVVIEVVFYSNTNTELSRERYKIYDFFPAHSTKAFE
                     LKINRPAACTKLGWTAVDATPY"
     gene            1564..2832
                     /locus_tag="VFU15_02630"
     CDS             1564..2832
                     /locus_tag="VFU15_02630"
                     /inference="COORDINATES: protein motif:HMM:NF022466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2851 family protein"
                     /protein_id="HEU4716694.1"
                     /translation="MTEEFLHHIWRTRQFNPANLRVTDGRALEIIYPGEHNIFSGPDF
                     FNAKLRIGDQLWAGNVEIHVKASDWMKHRHQEDEAYGNVVLHVVYEADREISARGNVL
                     PALELKERVPGKLWTRYSEMRGSKDRLACAGSMAGASSFTVAAMLGRAIVERLERKTD
                     EIRTELLHSGNDWEETFYRRLAHRFGMNVNAEPFHRLALSLPFRLLMRHADQPHQGEA
                     LLFGQAGLLDDAATDAYMSDLQREYRHLRKKYSLQSIETHAWKFMRLRPGNFPTLRIS
                     QLAALLTQRTQLFRACMEMKTAEELEAFFDVQASAYWDTHYRFRISSKECVKRLGAQA
                     TDVILINTVAPVKFLYGKMKKEERYCDEAIALLEEINAEENSVTRLFSAQAMELRNSA
                     DTQGALELKKNYCDRKRCLDCEIGRELLGR"
     gene            complement(2896..3141)
                     /locus_tag="VFU15_02635"
     CDS             complement(2896..3141)
                     /locus_tag="VFU15_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC family transcriptional regulator"
                     /protein_id="HEU4716695.1"
                     /translation="MTRIPFIDRVVSWFEQQAFGVCSWWGGKLGIRPTKIRMYFIYLS
                     FFTVGSPVIMYFIMAFMLEHKEYFRPKKYRKHSVWDL"
     gene            complement(3261..5429)
                     /gene="feoB"
                     /locus_tag="VFU15_02640"
     CDS             complement(3261..5429)
                     /gene="feoB"
                     /locus_tag="VFU15_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014682566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HEU4716696.1"
                     /translation="METKAAETGERKVALIGNPNSGKSTLFNALTGLHQKTGNFPGVT
                     VDKKTGTVKIRSGENFTTVHYTDLPGTYSLYPKSLDEEVATRVLTEKESEDFPDAVVI
                     VTDSTNLKRGLFLTGQVIDLNIPCVLALNMMDEAEKAGLHIDVNRLSNRLGIPVIPIS
                     AREKTGIDLLEKVIAQKVEEPAYMFADVSGIASEAIKLVKGGDHAMKDYQALHRANRL
                     FETNAEWREKLAAIGFSPSGAQAEESLLRYGVIGQHMSVCTGAHAERPQLTRRLDRLL
                     THKVWGYIFFLGLMLAVFQSIFFLAQYPMEWIEAGFAALQSFCAQHMPAGILTDLFIN
                     GILAGLSGVVVFVPQIALLFFFIAMLEDSGYMARVSFILDRLMRRIGLHGRSVIPLVS
                     GMACAVPAIMSTRTISNWKERIITIMVTPLMSCSARLPVYTLLISLVIPNKMVWGVFQ
                     LQALTLMGLYLLGFFMAILSAWVISRIIRAKEKSWFIMELPVYRTPKWSNVFYTIYEK
                     VKVFLFDAGKIIIAISIVLWVLASFGPGENFRSVDARITSIDVDLKNLSGNILPPDKT
                     VDQLAQWKTDREAEKKKLESKKLELSYAGHLGKMIEPVIRPLGYDWKMGIALITSLAA
                     REVFVGTMATIYGAGDDEDTQPIRERMRNERNPETGEKVYTPATGISLMIFYAFALQC
                     MSTIAVVRRETRSWKWPAIQFTYMGVLAYVASLLVFHLLK"
     gene            complement(5439..5687)
                     /locus_tag="VFU15_02645"
     CDS             complement(5439..5687)
                     /locus_tag="VFU15_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012926051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - obsolete iron ion homeostasis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HEU4716697.1"
                     /translation="MAEYRDNLASLGLGEKAVIDSFTDPELSLKLIEMGCTPGEEVLL
                     EKIAPMGDPIAINISGYVLSLRKAEAATVCIRRVNGKH"
     gene            5734..5940
                     /locus_tag="VFU15_02650"
     CDS             5734..5940
                     /locus_tag="VFU15_02650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716698.1"
                     /translation="MTIVPKSGYFPAKLLFLAGRGLRVHAGQRLPGAFFKKVPEKAAA
                     FHRGEREEAKQYRYDFLHRGSYRW"
     gene            complement(6097..6255)
                     /locus_tag="VFU15_02655"
     CDS             complement(6097..6255)
                     /locus_tag="VFU15_02655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716699.1"
                     /translation="MRRTVKKILSLLLVAGSISFLAPSCWVFHGKNHCGTCPDFKHND
                     KPKKVKKH"
     gene            6360..7109
                     /locus_tag="VFU15_02660"
     CDS             6360..7109
                     /locus_tag="VFU15_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010420644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU4716700.1"
                     /translation="MDPLSLFYHIGRYFMMMKRVFRMPERRSIFWNRFFHEVNAIGIG
                     SLGIVAIISIFMGAVITIQSAFGFESPWIPLYAVGVATRDSIILEFSPTIVSLILAGK
                     VGSSIASEIGNMRISEQIDALEVMGVNSASFLVLPKITASVFIFPFIVIISMFLGHVG
                     GFVLGVASGVVTPYEFVYGIQYDFRPYNVFYAIVKTIVFGFIIPSVSSYHGYFARGGS
                     LEVGQASTKAVVYSIIVLLLFNFILTQLLLA"
     gene            7106..7870
                     /locus_tag="VFU15_02665"
     CDS             7106..7870
                     /locus_tag="VFU15_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010420642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU4716701.1"
                     /translation="MIEVKNLNKSFGDKVVLRDFSFVFEQGKANLIIGQSGTGKSVLI
                     KCMVGLIAPESGEVCYDGKDFLHLDYDEKKVIRQQIGMLFQGGALFDSMTVEENVKFP
                     LNMFTTMTEAEKTERMHFCLERVNLPGAANLYPAELSGGMKKRVAIARAISMRPKYLF
                     CDEPNSGLDPLTSIVIDDLIKEITEEYNMTTVVITHDMNSVLEIGDKIAFLHKGDKAW
                     EGTRHEVLTSDNKILSDFVYANKFLKSFRDRIQKGQ"
     gene            complement(7921..9240)
                     /locus_tag="VFU15_02670"
     CDS             complement(7921..9240)
                     /locus_tag="VFU15_02670"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEU4716702.1"
                     /translation="MKKIYTLAFLALGSAAAVNAQQLVTPTQNQVRDITHAQFDRSKA
                     PNQRTAPVVTTQSFSGWVSYADAKDNDLGGGVSVLGGTYLFPDSTVQADFGAGTYDHP
                     WVHSIGDVFDVAAPSFSNLYAGYSLGRTDAYSIDSIAIVYTYGRMSPSPNADTLICHV
                     YYNSNGTNLTNNGFIGTTAANYGTDTVSFKQQHYTYQTNTPTASGSVQVYKIPLTIAD
                     TTLAFYGTKAFYTGYGVPAGKIAAITFEFVPGYSYNFGDTITLNKNYFTFASTEEAGT
                     NTYPIYYDCNYQGTDCDFNCSSVIRTQERYNIDPAQSWNGLYIPKYAFTQAYGLENHY
                     IYYYITSPPAGIAGPEQAAGFSLGQNVPNPATGNTSISYTLANEGNVALTIYDITGKQ
                     VMLFNEGAQNAGTYKVDVNTNSLQAGVYFYELNVDGKKAVRKMVISE"
     gene            complement(9388..10434)
                     /locus_tag="VFU15_02675"
     CDS             complement(9388..10434)
                     /locus_tag="VFU15_02675"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEU4716703.1"
                     /translation="MKKIYMLAAAVAMVFAANAQQSNTKAAPNMPSTVMPMPSQTPQV
                     IHHAPTHTDAVGDTVFVFDGYYFYDWNQTLPASFMYQTEDLDGLAINSALSAYFGSTG
                     DFVYFFEQNPTSNLSYAHPDSVFFAAATSWFTSPGQADNWMEFGPVVVPAAGGTLKWR
                     NNMPDGNFRDGYEVYVSTTGMNNYSDFTGSPVFSVADNAPSTAGDTVNTPYNVFVQKT
                     ADISSYAGQQVYVGIHHNANDQFILYLTDIMLVEGPAGVNNTADEVNLFTATPNPANN
                     SSFINFNLKNAGDVTLNVTDVNGKVVYSENQANQAAGNHTIEVNTTDFANGVYFYTVT
                     ANGVAKTQKLVVTH"
     gene            complement(10540..13392)
                     /gene="gcvP"
                     /locus_tag="VFU15_02680"
     CDS             complement(10540..13392)
                     /gene="gcvP"
                     /locus_tag="VFU15_02680"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014032050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HEU4716704.1"
                     /translation="MSTDKFTGRHIGPSAQEKNEMLNAIGVASTEELIGQVIPSSIRL
                     KKPLNIGQGLSEYEYLRHLGEIAAKNKVFRSCIGLGYYGTITPPVIQRNILENPGWYT
                     AYTPYQAEISQGRLEALLNFQTMVMDLTAMPLANASLLDEATAAAEAMAMLFSSRSRD
                     AVKRGAVKFFASEKCFPQTIDVLRTRSTPLGIELVTGDHTTFRWTDEFFGGLVQYPDA
                     DGDINDFRAFTDDAHASGAGVAVATDLLALTLLTPPGEWGADVVFGNSQRFGVPMGYG
                     GPHAAFFAAKEDFKRHMPGRIIGVSVDSHNRPALRMALQTREQHIKRDKATSNICTAQ
                     ALLAIMASMYAVYHGPSGLRAIAEDIHEKTARLSSSMEKAGWKKINSRFFDTISFEIP
                     AGNSVEKIRTAAENASVNFRYGSATIHISIDQCILENDLQDIFNVLGVKDASAAGAGF
                     GSFARKSSYLTHPVFNTYHSETAMMRYIKKLENKDLSLVHSMISLGSCTMKLNAAAEL
                     LPLTWPGFANIHPFVPAGQAEGYRTMIGELDKSLSEITGFAAMSFQPNSGAQGEYAGL
                     MVIRAYHISRGDNQRNIALIPSSAHGTNPASAAMAGMEIVVVKCDEKGNIDVADLKAK
                     AELHRNKLSCLMVTYPSTHGVYEESITEITSIIHANGGQVYMDGANMNAQVGLTSPGN
                     IGADVCHLNLHKTFAIPHGGGGPGMGPIGVAKHLVPFLPSHSVVNTGGEKGIHAVSAA
                     PYGSALILLISYGYIKMLGGDGLRESTEMAILNANYIKEKLKGHYSTLYTNHNGRVAH
                     EMILECAEFKRTAKVEVGDIAKRLMDYGFHAPTVAFPVHDTLMVEPTESEPKAELDRF
                     CDAMIAIRNEIDEIASGKSDVADNVLKNAPHTCESVIAGEWKHPYSREKAVYPLSWTK
                     ENKFWPSVGRVDNAHGDRNLICSCPPVEAYMEPVTA"
     gene            13516..14154
                     /locus_tag="VFU15_02685"
     CDS             13516..14154
                     /locus_tag="VFU15_02685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716705.1"
                     /translation="MKNFRALTAFLFLAMPLFAQTGSQPMDTVYLMSGNTVAGVVKDS
                     SDDELKILIPKKDGSFKADFIDLDLVFSVKYKNGQESVFYKQDTLFGNYYSEDEVRYF
                     IYGERDARSHFRCPVWNAGAFAAGFAGGWTQSLFMFVPPFAYAGITTFFRVKIKPGTV
                     GRPEYLQRDTYLLGYEKDARKRRIMRSLIWGGIGMAMGYTSSYFYYQNHSTN"
     gene            14188..>14578
                     /locus_tag="VFU15_02690"
     CDS             14188..>14578
                     /locus_tag="VFU15_02690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716706.1"
                     /translation="MKKFLRWLFDFYLFGNIHIAVCAVALLLCTKLFFGMELRRELLV
                     FVFNGTFFLYSLQRIPAAFGGEKVERRFRRHHWNIEHKYALAALCVIAAVLSGWSFFR
                     LYLRSQLVAMVPAFLSFIYAFPVVPSRK"
BASE COUNT         3243 a         3676 c         4030 g         3629 t
ORIGIN      
        1 gtggatcgtt ttccgtgtga ccccgagatc taccccgtat ccggttctta tcctttactg
       61 gaatccgaag gttacgacga aaaaactatc ggtctgattt caacgcctgc aggttttgta
      121 tctggtcctg caatgatttt atttctgcgt ttttcacgtc aatcttcttc agttgatcgc
      181 tttcttcctt tgccgccgca tcaaaggctg cgctggcatc ttttccttcc cagaatttcc
      241 catccttatc gatccagcca tagcttccgc cggtagaaac tctggccctt acggtggcga
      301 gcctggtttt gacgtacaaa taatcgggat cattttccaa acggtaaatt tcccgctgtt
      361 gtttcacgat ctcagaatca agcacgttta cctggcttga aaccattgag tcgttcttcc
      421 gccgggaagc atcgatggta gcgtgtgccg tactgtccat tttggctttt tcgacctcag
      481 ctctttcctg tgcgcttggt ccgcaggaaa tgaactgtat caggatgatc ggtaataaaa
      541 ttcgaaatgg tgttttcata gcagggttca atgaaggaat gaagactgta ttaaaattga
      601 aacagcaaca cgattcttct aatacggagt tgcatctacc gctgtccatc caagttttgt
      661 gcaggcagcc ggtctgttta ttttcagttc aaaggctttt gttgaatgtg ccggaaaaaa
      721 atcatatatt ttatatcgtt cacgactcaa ttctgtgttg gtgtttgaat aaaacactac
      781 ttcgataacg acatctttat agttagcaac tgtagcttta ttctttacat ttccgtgaat
      841 tttcatagca tcaccccaga agttctgatt atatgtaccg ccggcatcaa gaaactgcgc
      901 cggattttcc cgttcgattt cttcggctga tttaatggtt tcctgataat tggtggaatt
      961 tggcccagaa gagtgatctg agcctatgtg tttttgtatc gctgaaaaaa tgagaattag
     1021 taaaagcact cctccaatta tagataaaat aataccagta gggtttttgg ctttgggtga
     1081 tgtctgagaa ggagccttgg aactctggac cggttctttt ttagttaagg gaggtggggt
     1141 ggataaattt tgcttttcaa agggtggggg tacaggggcg cgaaatattg tgttaagttc
     1201 agctacactc ccagccgttg tccatttctc caaaccttca taccaaagag gggtgtcttt
     1261 attaatattc ttagccttaa gctcgtcaat gctgaaaggc ccatcctggt tattaccatc
     1321 gtggatataa tattttttca tttataaatg tgtttatcgg gttgatgttt tacagactaa
     1381 aacaaatcta cttttcttca tagagtaaat caagtagtac tgtatgtttt tgggctaaat
     1441 aaactatttg atactgtcta attgtaaacg tttgctgtcg tttatatacg gataaaaccg
     1501 tttgctgctt gctcacaggg ttctctcccg ttttctttgc gccctttttc agaagaaagc
     1561 aacatgaccg aagaattcct gcatcatatc tggcgcacga gacagttcaa cccggccaac
     1621 ctgcgcgtga cggacggacg cgcactcgag atcatctacc cgggcgaaca caatattttt
     1681 tccggtccgg attttttcaa cgcgaagctg cgcatcggcg atcagctgtg ggcgggcaac
     1741 gtggagatac atgtgaaagc gtctgactgg atgaagcacc ggcaccagga ggatgaagcg
     1801 tacggcaacg tcgtgctgca cgtggtgtac gaagccgacc gggagatctc cgcgcgggga
     1861 aatgtgctgc ctgcgctcga gctgaaagaa cgcgtgccgg gaaaactgtg gacgcgttat
     1921 tcggaaatgc gcggcagcaa agaccggctc gcttgcgcgg gctcgatggc gggcgcgtct
     1981 tcgttcacgg tggcggctat gctcggtcgc gcgatcgtgg aacggctcga acgcaaaacg
     2041 gacgagatca ggaccgaatt gctgcacagc ggcaatgact gggaagaaac attttaccgc
     2101 cgcctggcgc accgtttcgg gatgaacgtg aatgcagaac cgtttcaccg tctcgcgctt
     2161 tcgcttccgt tccgcctcct catgcgtcac gccgatcagc cgcaccaggg cgaagcgctg
     2221 ctcttcgggc aggcgggatt gcttgatgac gcggcaacgg atgcgtacat gtcggacctg
     2281 cagcgggagt accgtcacct gcggaagaaa tacagcctgc agtccatcga aacgcacgcc
     2341 tggaaattca tgcgcctgcg accgggaaat tttcccacgc tgcgcatttc ccagctcgcg
     2401 gcattgctca cgcagcgcac gcaattattc cgtgcgtgca tggaaatgaa aaccgcggaa
     2461 gagctggaag catttttcga tgtgcaggcg tccgcttact gggacacgca ttaccgtttc
     2521 aggatttctt cgaaggaatg cgtgaaacgt ctcggcgcgc aggcaacgga tgttatcctc
     2581 atcaacacgg tggcaccggt gaagttcctg tacggcaaaa tgaaaaaaga agaacgttac
     2641 tgcgatgaag cgatcgcact gctggaagaa ataaatgccg aagagaattc agtcacgcgc
     2701 ctgttttccg cccaggcgat ggagctccgc aattccgccg atacgcaggg tgcgctggag
     2761 ctgaaaaaaa attattgcga ccggaaacgc tgcctcgatt gtgagatcgg gcgggaactg
     2821 ctgggaaggt gagtgaattt caaatggctg cgcaatacag gcgaagttcc ggaaccgggc
     2881 ccggccacgt ggcgatcaca ggtcccacac gctgtgcttc ctgtattttt tcggacggaa
     2941 atattctttg tgctcgagca tgaacgccat gatgaaatac atgatcacgg gcgaacctac
     3001 ggtgaaaaaa gaaaggtaga tgaaatacat cctgatcttc gtcgggcgta tgccgagctt
     3061 gccgccccac caggaacaga cgccgaacgc ctgctgttcg aaccaggaaa caacgcgatc
     3121 gataaaagga atgcgtgtca taagcgcaaa actataccta ctaaaataag aaaaaaacag
     3181 cgcaggaaaa taagccggga aagttaaaaa ttgaaaaggc gggaagaagc tggaaagttg
     3241 ttcgggttca gcagctctcc ttatttgagc aggtgaaaaa cgagtaggct cgccacgtaa
     3301 gcgagcacgc ccatgtaggt gaactggatg gcgggccatt tccagctgcg ggtttcgcga
     3361 cgtaccacgg cgatcgtgct catgcactgc aaagcgaatg cgtagaagat catcagtgaa
     3421 atgccggtgg cgggcgtgta tactttttct cccgtttcag ggttgcgttc gttgcgcatg
     3481 cgttcgcgga tgggctgcgt gtcttcatcg tcgcctgcgc cgtagatcgt ggccattgtt
     3541 cccacgaaaa cttcacgcgc tgcaagagaa gtgatgagcg caatgcccat tttccagtcg
     3601 tagcccagcg ggcgtatcac gggttcgatc attttcccga gatgcccggc gtacgaaagc
     3661 tccagctttt tcgattcgag ctttttcttt tcggcttcgc ggtccgtttt ccattgcgcg
     3721 agctggtcta ccgttttatc cggcggaagg atgttgccgg aaagattttt caggtctacg
     3781 tcgatcgacg tgatgcgcgc atccacgctg cggaaatttt cgccgggccc gaacgaggcc
     3841 agtacccaga gcacgatcga gatcgcgatg atgatcttgc cggcgtcgaa tagaaacact
     3901 ttcacttttt cgtagatcgt gtagaacacg ttgctccatt tcggcgtgcg gtacaccggc
     3961 agttccatga tgaaccagct tttttctttt gcgcggatga tgcggctgat cacccacgcg
     4021 gaaaggatcg ccatgaaaaa gccgagcagg taaagtccca tcagcgtcag cgcctgcagc
     4081 tggaaaacgc cccacaccat tttgttcggg atgacgagcg agatcagcag tgtgtaaacg
     4141 gggaggcgtg cggagcagct catcagcggc gtgaccatga tcgtgatgat gcgttccttc
     4201 cagttgctga tcgtgcgcgt gctcatgatc gcgggaacag cgcaggccat gccgctcacg
     4261 agcgggataa cggagcgtcc gtgcaggccg atgcgccgca tgaggcggtc gaggatgaag
     4321 ctcacgcgcg ccatgtagcc ggaatcttcg agcatcgcta tgaaaaagaa aagcagcgcg
     4381 atctgcggaa cgaaaaccac cacgccgctc agcccggcta aaatcccgtt gatgaaaaga
     4441 tcggtgagga tacctgcggg catgtgctgt gcgcagaacg attgcagcgc agcgaagccg
     4501 gcttcgatcc attccatcgg gtactgcgca agaaaaaaga tcgactggaa aacagcgagc
     4561 atgagcccga ggaaaaaaat atagccccac actttgtgcg tgagcagccg gtcgagcctg
     4621 cgtgtgagct gcggacgttc cgcatgcgcg cccgtgcata cgctcatgtg ctggccgatg
     4681 acgccgtaac gcaaaagact ttcctcggcc tgcgcgccgg aaggactgaa gccgatcgcg
     4741 gcgagctttt ccctccattc cgcgttcgtt tcgaacaggc ggttggcgcg gtgcagcgcc
     4801 tggtaatctt tcatcgcgtg gtcgccgcct ttgacgagct tgatcgcttc ggaggcaatg
     4861 ccggaaacat cggcgaacat gtacgcgggc tcttccactt tctgcgcgat gactttttca
     4921 agaagatcaa ttccggtttt ttcccgcgcg ctgatcggga tcaccgggat tccgagacgg
     4981 ttcgagagac ggttcacgtc gatgtgaagt cccgcttttt ccgcttcgtc catcatgttc
     5041 agcgcaagca cgcagggaat gttcaggtcg atcacctgtc ccgtgagaaa aagtccgcgc
     5101 ttgagattgg tggaatcggt gacgatgacg acggcatccg gaaaatcttc gctttccttt
     5161 tccgtaagca cgcgcgtcgc cacttcttcg tcaagcgatt tcgggtaaag actgtacgtg
     5221 cccggaagat cggtgtaatg gaccgtcgtg aaattttcgc cggaacgtat tttcaccgtc
     5281 cccgtttttt tgtcgacggt gacgccgggg aaatttcccg ttttctggtg aaggcccgta
     5341 agtgcgttga agagcgtgga cttgccgctg ttggggttgc cgatgagcgc aaccttccgt
     5401 tcaccggttt cagcagcttt tgtttccacg aaagaagatc agtgttttcc gttcacgcgg
     5461 cgaatgcaca ccgtcgcggc ttccgctttg cgcaggctca gcacataacc ggaaatgttg
     5521 atggcgatcg ggtcgcccat gggcgcgatt ttctccagta atacttcttc gcccggcgta
     5581 cagcccatct cgatcagctt cagcgaaagc tcaggatccg taaacgagtc gatcactgct
     5641 ttttccccta atcccagcga cgccaggtta tctctgtatt ctgccataaa attcacgtca
     5701 ggcaaagata acgtacgagg ctccccgccg gaaatgacaa ttgtacctaa aagtgggtat
     5761 tttccggcga agcttttatt tcttgccggc cggggtcttc gcgtgcatgc cgggcaaagg
     5821 ttgccaggcg ccttttttaa aaaagtcccg gaaaaagccg cagctttcca tagaggtgag
     5881 cgtgaagaag caaagcagta tcgttatgat tttcttcatc gcggcagcta tcgctggtga
     5941 gcgccctttc gcggtttcgg gtcgttcacg tgcccgcaat ccccgcaaat gttcttgtgg
     6001 aacagccggc aaccttcgga agccgttccg atcacggaaa caagagcaag aagcaggagg
     6061 aatcgtctca tggtgtaaag gcaaccccgg gatccgtcag tgttttttga cttttttcgg
     6121 cttgtcgtta tgcttgaaat ccgggcaggt gccgcagtgg ttttttccgt ggaaaaccca
     6181 gcaggaaggc gccaggaacg aaatgcttcc ggcgaccagc agcagggaga ggattttttt
     6241 tacagtgcgg cgcatctttc aaatttactc ttttccggag cctgttccaa tactcaaacg
     6301 gcccgcacgg gccggaaagt acccgctgcc ttcattattt gtaaatttgc cgttcttcga
     6361 tggatccgct cagtcttttt taccatatag gccgctattt catgatgatg aagcgagtgt
     6421 tccgcatgcc agaacgccgc agcattttct ggaaccgctt ttttcacgag gtcaatgcca
     6481 tcggcatcgg ctcgctgggc atcgtggcga tcatttccat cttcatgggc gccgttatca
     6541 ccatccagtc ggcattcggt ttcgaaagcc cgtggatccc gctctatgcc gtgggcgtcg
     6601 ccacccgcga ttccatcatc ctcgaatttt cgccaaccat cgtcagcctt attctcgccg
     6661 ggaaagtggg ttcgagcatt gcctccgaga tcggcaacat gcgcatttcc gagcagatcg
     6721 atgcgctgga ggtcatgggg gtgaactccg ccagcttcct cgtgctgccg aaaataacgg
     6781 cttctgtttt catcttcccg ttcattgtca tcatcagcat gtttctcggg catgtcgggg
     6841 gattcgtgct gggcgtggct tcgggcgtgg taacaccgta cgagttcgtc tacggcattc
     6901 aatacgattt ccgtccgtac aacgtgtttt acgccattgt caaaaccatc gtattcgggt
     6961 tcatcatccc gtctgtttcg tcgtaccacg ggtatttcgc ccgcggcgga tcgctcgagg
     7021 tgggacaggc cagcaccaag gcggttgtct acagcatcat cgtcctcctg cttttcaatt
     7081 tcattcttac acagcttctc ctcgcatgat cgaagtgaaa aatctgaaca aatcattcgg
     7141 ggacaaagtt gtcctgcgtg atttttcatt tgtgttcgag cagggaaaag caaacctcat
     7201 catcgggcag tcgggaacgg gaaaatccgt gctcatcaaa tgcatggtgg gactcatcgc
     7261 gccggaaagc ggcgaagtgt gctacgacgg aaaagatttt cttcatctcg attacgacga
     7321 gaaaaaagtc atccgccagc agatcgggat gctgttccag ggcggtgcgc tgttcgattc
     7381 gatgaccgtc gaggagaacg tgaagtttcc gctgaacatg ttcaccacga tgacggaagc
     7441 ggaaaaaacg gagcgcatgc atttctgcct tgaacgggtg aacctgcccg gcgcagcgaa
     7501 tctttacccg gcagagctca gcggcgggat gaagaaacgc gtggcgatcg cgcgagccat
     7561 ctccatgcgg ccgaaatacc ttttctgcga cgagccgaat tccggcctcg atccgctcac
     7621 ttccattgtc atcgacgatc ttatcaaaga gatcacggaa gaatacaaca tgacgaccgt
     7681 ggtgatcacg cacgacatga actcagtgct cgagatcggc gataagatcg cgttcctcca
     7741 caaaggcgac aaagcctggg aagggacgcg ccacgaagtg ctgacttccg acaacaagat
     7801 cctctcggat tttgtttacg cgaacaaatt cctgaaatcc ttccgcgacc gcattcagaa
     7861 agggcaataa aaaaacctct tcgtttttga agaggttttt ttataagaag aaaagtttcc
     7921 ttattcggag atcaccattt tacgcacggc tttctttccg tctacgttaa gctcgtagaa
     7981 gtaaacgcct gcctgcagtg aattcgtgtt gacatccact ttgtaggttc ctgcgttctg
     8041 agcgccttcg ttgaacagca ttacctgctt gccggtgatg tcatagatcg tcagcgcgac
     8101 atttccttca ttggcgagcg tgtagctgat gctcgtattg cccgttgccg ggttcggaac
     8161 gttctgtccg agcgagaagc cggcagcctg ctcaggtccg gcaatgcctg ccggcgggct
     8221 ggtgatgtaa taatagatgt agtggttttc gaggccgtaa gcctgcgtga acgcatattt
     8281 cggaatgtac agcccgttcc aggattgcgc gggatcgatg ttgtaacgtt cctgtgtgcg
     8341 gattaccgaa gagcagttga agtcgcagtc cgttccctgg tagttgcaat cgtaatagat
     8401 cgggtacgtg ttggttcccg cttcctcggt tgaagcgaac gtgaagtaat ttttgttcag
     8461 cgtgatcgtg tcaccgaaat tgtaggaata gcccggaacg aactcgaacg ttattgcagc
     8521 gatcttgccg gcaggaacac cgtagccggt atagaaagct ttcgtgccgt agaacgccag
     8581 cgtggtgtcg gcgatcgtca gcggaatttt gtacacctgc acgctgcccg atgcggtcgg
     8641 cgtgttggtc tggtaggtgt aatgctgctg cttgaaggaa acggtgtccg tgccgtagtt
     8701 ggcagccgtc gttccgatga agccgttgtt cgtgaggttg gtcccgttgc tgttgtagta
     8761 aacgtggcag atcagggtgt ccgcgttcgg gctcgggctc atgcggccgt aggtgtatac
     8821 gatggcaatg gaatcgatgg agtaagcatc cgtacgtccg aggctgtatc ctgcataaag
     8881 gttgctgaag gaaggagcgg cgacgtcaaa cacatcaccg atgctgtgca cccagggatg
     8941 atcgtatgtt cctgcgccga aatccgcctg tacggtggaa tccgggaaga ggtatgtccc
     9001 gccgagaacg gaaacgcctc cgccgagatc gttgtctttt gcatccgcgt agctcaccca
     9061 tccgctgaag gattgcgtgg ttaccacggg agcagtacgc tggttcggcg ccttgctccg
     9121 gtcgaactga gcatgcgtaa tgtcacgaac ctggttttgt gtcggcgtaa caagctgctg
     9181 agcatttacg gccgcggcac tgccaagtgc caggaaagca agcgtgtaga tttttttcat
     9241 ctcgtttatt tgttgggttt cgggccgcac atgcggcgta ttctaacaaa aatagcaaat
     9301 aaacatctct gaaaaaaaag tgtgatgaac ggtaataaaa aaagcggccc cggaggagcc
     9361 gcttttctat gtaactgaaa cccggaatta gtgggtaact acgagcttct gtgttttagc
     9421 aacaccgttt gcagtaaccg tgtagaagta tacgccgtta gcgaaatcgg tcgtgttcac
     9481 ttcgatcgtg tggttgcctg cagcctggtt agcctggttc tcggaataaa caacttttcc
     9541 gttcacatcc gttacgttga gcgtaacgtc acctgcattt ttcaggttga agttgatgaa
     9601 agaagagttg ttggccgggt tcggcgtagc ggtgaagaga tttacttcgt cagcggtgtt
     9661 gtttacacca gcgggacctt caacgagcat gatgtcggtg aggtacagga tgaactggtc
     9721 gttcgcgttg tggtggatac ccacgtacac ctgctgtcct gcatagctgc tgatgtcagc
     9781 tgttttctga acgaaaacgt tgtacggagt gtttaccgtg tcaccagctg ttgagggagc
     9841 attgtcagct acactgaaaa ccgggcttcc ggtgaaatca gagtaattgt tcattcccgt
     9901 ggtggaaacg tatacttcat agccgtcacg gaaattgccg tcgggcatgt tgtttctcca
     9961 cttgagcgtg ccgcccgcag caggaacaac aaccggtccg aattccatcc agttgtcagc
    10021 ctgtccgggc gatgtgaacc aggaagttgc cgcagcgaag aatacgctgt cagggtgggc
    10081 atacgacagg ttagaggtcg ggttctgctc aaagaagtaa acgaaatcac ccgtgcttcc
    10141 gaaataagcg gaaagcgcag agttgatcgc aagaccgtcg aggtcttccg tctggtacat
    10201 gaaagaagcc ggaagtgtct ggttccagtc atagaagtaa taaccgtcga atacgaaaac
    10261 ggtatcaccc acagcatctg tatgtgtcgg tgcgtggtgg atgacctgcg gagtctgcga
    10321 aggcatcggc attaccgttg aaggcatgtt cggcgcagct ttcgtgttgc tctgctgagc
    10381 attcgctgcg aataccattg caacggctgc agcaagcatg taaattttct tcattgtttt
    10441 ttgtttagtt ggttttggaa tcgttgtcta aaggcaaaaa taaggattta ttaaaactcc
    10501 accaattccc ggccggatgg tgttttttaa caaatcagct cacgcggtga ccggctccat
    10561 gtacgcttcc accggcgggc acgaacagat gaggttgcgg tcgccatgcg cattgtccac
    10621 acgcccgacc gagggccaga atttattttc cttcgtccag ctcagcgggt aaacggcttt
    10681 ttcacgggaa tacggatgct tccattcccc ggcgatcacg ctttcgcagg tatgcggcgc
    10741 atttttgaga acattatccg ccacatcgga ttttccactg gcgatctcgt cgatctcgtt
    10801 gcggatagcg atcatcgcat cgcagaaacg gtcaagctcc gctttgggtt cgctttccgt
    10861 cggctccacc atcaacgtat cgtgaacggg gaacgctacc gtgggtgcat gaaagccgta
    10921 atccattaag cgtttggcaa tatcgccgac ctccactttc gcggtgcgtt tgaattccgc
    10981 gcattcgagg atcatttcgt gcgccacgcg cccgttgtgg tttgtataca gcgtgctgta
    11041 atgtcctttc agcttttcct tgatatagtt cgcattcagg atcgccattt ccgtgctttc
    11101 acgaagcccg tcaccgccga gcattttgat atacccatag gagataagca ggataagcgc
    11161 gcttccgtag ggagctgcgg aaacagcatg aatacctttt tccccgcccg tgtttacgac
    11221 ggagtgcgaa ggcaggaaag gaacgagatg cttcgccacg ccgatgggac ccattcccgg
    11281 accgccaccg ccatggggaa tagcaaatgt cttgtgcagg ttcaggtggc acacgtctgc
    11341 gccgatgttc cccggtgatg tgaggccgac ctgcgcattc atgttggctc cgtccatgta
    11401 tacctggccg ccgttcgcgt ggatgatgga cgtgatctcg gtaatggatt cttcataaac
    11461 gccgtgcgtg gaaggatacg ttaccatgag gcaggaaagc ttgttgcggt gcagctctgc
    11521 ttttgcttta agatcggcga cgtcgatatt ccctttttca tcgcatttca caacgacgat
    11581 ctccattcct gccatagcgg cggaagcggg gttcgtaccg tgcgcggaag aaggtatgag
    11641 cgcgatgttg cgctggttgt cgccccggct gatgtgatag gcgcggatca ccatcagccc
    11701 ggcatattcg ccctgtgcgc cggaattcgg ctggaacgac atggccgcaa agccggttat
    11761 ctcgctcagc gatttatcca gctcgccgat catcgtgcga tatccttctg cctggccggc
    11821 cggaacgaac gggtgaatat tcgcgaagcc gggccacgtc agcggaagaa gctcggccgc
    11881 tgcgttaagt ttcattgtgc aggaaccgag cgagatcatg gaatgcacga gcgaaaggtc
    11941 tttgttctcg agcttcttga tgtaacgcat catggcggtt tccgaatgat acgtattgaa
    12001 cacggggtgc gtgaggtagc tgcttttccg tgcgaaagaa ccaaatcctg cgccggcagc
    12061 ggaagcatcc ttaacaccga gcacattgaa aatatcctgc aggtcatttt ccagaatgca
    12121 ctggtcgatg ctgatgtgaa tagttgcgct gccgtaacgg aaattgacgg aagcgttttc
    12181 tgctgcggtg cggatcttct caacagaatt tcccgcagga atttcgaacg agatcgtatc
    12241 gaagaagcgg ctgttgatct ttttccatcc tgctttttcc attgacgagg aaagccgtgc
    12301 ggttttttcg tgaatgtctt ccgctatggc gcgcagaccg gacgggccat gataaacggc
    12361 gtacatgctg gccatgatgg cgagcagcgc ctgtgcggtg cagatgttcg aggtggcctt
    12421 gtcgcgcttg atgtgctgct cgcgcgtctg gagggccatg cgcagcgcag gcctgttgtg
    12481 gctgtcgacg gaaacgccga tgatgcgccc cggcatgtga cgtttgaaat cttctttcgc
    12541 tgcgaagaac gctgcgtgag gtccgccgta acccatgggc acgccgaaac gctgggaatt
    12601 cccgaacacg acgtcagcgc cccattcacc gggaggagtg agcagtgtga gcgcaagaag
    12661 atccgtagcc acggccacgc ccgcaccaga agcatgcgcg tcatctgtga atgcacggaa
    12721 atcgttgatg tctccgtctg catcgggata ctggacaagt ccgccgaaga attcgtccgt
    12781 ccaccggaat gtggtatggt cgccggtcac gagctcaatc ccgagcgggg tggaacgtgt
    12841 gcgcagcaca tcgatggttt gcgggaagca tttttcagag gcaaagaatt tcacggcgcc
    12901 gcgtttcacg gcatcgcggc tgcggctgct gaagagcatg gccatggctt ccgcagctgc
    12961 ggtcgcttcg tcgagcaggg aagcgttggc aagcggcatt gccgtaaggt ccattaccat
    13021 cgtctggaaa ttcagcagcg cttcgaggcg gccctgggaa atttccgcct ggtaaggtgt
    13081 gtaagcggtg taccagcccg ggttttcgag aatgttgcgc tggatgacag gaggagtgat
    13141 cgtgccgtaa taaccgagcc cgatgcagga ccggaaaact ttatttttcg ctgcgatctc
    13201 gcccaggtgg cggaggtatt cgtattcgga aagaccctgc ccgatgttca gcggcttttt
    13261 cagccggatg gaagaaggga tgacctggcc gatcagttct tcggtggaag caacgcctat
    13321 cgcgttcagc atttcatttt tttcctgtgc ggaagggccg atatggcggc cggtaaattt
    13381 atctgttgac atgttttttt gtggaatgat cgtctggaaa aagggaaaca aatatacgca
    13441 gaaagaaaac ccgaaaaccg ggcggatgac gggtaagacg gatcgttttt tatctttgga
    13501 aaagaaaccg agaaaatgaa gaatttccgt gcgctgaccg ctttcctttt tcttgccatg
    13561 cctttgttcg cccagaccgg ttcacaaccg atggatactg tttacctgat gagcggaaac
    13621 acggtggccg gcgtcgtgaa agattccagc gacgatgaac tgaagatact tattcccaaa
    13681 aaggacggct ctttcaaggc agacttcatc gatcttgatc tcgtgttttc cgtgaaatac
    13741 aaaaacggcc aggaatccgt cttttataag caggacacgc tcttcggaaa ttactacagc
    13801 gaggacgagg tgcggtattt catttacgga gagcgtgatg cccggagcca tttccgttgc
    13861 ccggtatgga acgcgggcgc ttttgcggcg gggtttgccg gcggctggac acaaagtctt
    13921 ttcatgttcg ttccgccttt tgcctacgcg ggaatcacca cttttttccg tgtgaaaata
    13981 aaacccggca ccgtgggcag gcccgaatac ctgcagcggg acacttatct tctcgggtat
    14041 gaaaaggatg cgcgaaaacg gcggatcatg cgttccctca tctggggcgg gataggaatg
    14101 gcgatgggat acacttcatc ctacttttat taccagaatc attcgacgaa ctgaggaaag
    14161 gccggagcag ggccggcatg actgcgaatg aaaaaatttc tccgctggct cttcgatttt
    14221 tacctgttcg gcaatattca tattgcggtg tgcgccgtcg cgctgctgct ctgcacgaag
    14281 ctgtttttcg ggatggagct gcgcagggag ctgcttgttt tcgttttcaa cggcacgttt
    14341 ttcctgtaca gtcttcaacg catcccggcc gcgttcggcg gtgaaaaagt ggagcggcgg
    14401 tttcgccggc atcactggaa catcgagcat aaatatgcgc tcgccgcttt gtgcgtgatc
    14461 gctgcggtcc tttcgggctg gagctttttc aggctgtacc tgcgctcgca gctggtggcg
    14521 atggtgcccg cattcctttc cttcatttac gcgttcccgg tcgttccgtc gcggaaag
//
LOCUS       DASTSF010000090         4039 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1506377,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000090 DASTSF010000000
VERSION     DASTSF010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4039
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1506377"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..555
                     /locus_tag="VFU15_02695"
     CDS             <1..555
                     /locus_tag="VFU15_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018128011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase hexapeptide repeat family protein"
                     /protein_id="HEU4716707.1"
                     /translation="AFVHPQATVTGNVIIGKKVYIGPGAAIRGDWGEIIIEDGCNVQE
                     NCTIHMFPGISVKLMEGAHIGHGAVIHGATIGRNCLVGMNSVIMDDVVLGDECIVGAL
                     TLIRADTVIPPRKLVVGNPARIAKDVSDDMIAWKTKGTELYQQLPAECRATLKECEPL
                     REVPVNRPKQQNIYRTWGESRNDG"
     gene            604..945
                     /locus_tag="VFU15_02700"
     CDS             604..945
                     /locus_tag="VFU15_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009285450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2304 domain-containing protein"
                     /protein_id="HEU4716708.1"
                     /translation="MERVQIIAIIASLAFLVFISSLIVRGRLREEYAIIWIVCSALLI
                     AFSFWRKGLDVMSKLIGVYDPPNLVFTGAIFAVMIYLLHLSVVVSRLQEQNKTLSQDI
                     ALLKEKMKEKK"
     gene            942..1334
                     /locus_tag="VFU15_02705"
     CDS             942..1334
                     /locus_tag="VFU15_02705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716709.1"
                     /translation="MSVDHGKEALVLGKYLLGGKIPDEKSVSLYVAAHQKKPVSLDAR
                     EEKIFAFLLRHPRCCGVIDSALAVSNPHGGIRRKIMYMSAILEARPAWADLFLPRERS
                     FFYNLFIFWIGARALLKIFAGKILLAFV"
     gene            complement(1383..1946)
                     /locus_tag="VFU15_02710"
     CDS             complement(1383..1946)
                     /locus_tag="VFU15_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012721284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEU4716710.1"
                     /translation="MTNRLVEINKPEDIFPEYRNTPIGLLLEYHNLGRELEPYTQAKL
                     LIGMCMDNRKHLRIPDNFAFIIRAGGANLRYSEFKVSYAIAVGDIHHIVIIGHNNCGM
                     VNLVSRRQLFIDGLVEKAGWDRRQAEEHFMHYEPMHEIGNEIDFSISEAKRLRLRYPK
                     ITVAPLLYKVEDNRLYLIREKYEPVKG"
     gene            complement(2081..2773)
                     /locus_tag="VFU15_02715"
     CDS             complement(2081..2773)
                     /locus_tag="VFU15_02715"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU4716711.1"
                     /translation="MNRNLTNSIRFVLDEFLPPVIRDSRFFMYPLFFIWYKGKNVSKL
                     MRFKERAGKMTDREYAGYYEMYDAIANRETDLNRESIQFILDQSAENRHDRIIDVGCG
                     KGFLLKLLKENGYTDISGCDIIREMKDDSIPFTEGKIEQLPFPDKSFDVVICNHTIEH
                     ITDSRKAIAELKRIARKKIIITTPRQKYYRYTFDLHVNFFPQQSDLLHLISMPGTCVN
                     SNGDWSFAGNVQ"
     gene            complement(2818..3561)
                     /gene="arr"
                     /locus_tag="VFU15_02720"
     CDS             complement(2818..3561)
                     /gene="arr"
                     /locus_tag="VFU15_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007357792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)--rifampin ADP-ribosyltransferase"
                     /protein_id="HEU4716712.1"
                     /translation="MEFSPNNNIVKLCLQGMDMEEKGNPEEAGRLFLRAWNEATNDFE
                     KFLAAYYVARRQANVTDKLKWLETALQSALKINDVSVAGAFPSLYLNIAGCYEELGDF
                     DSAKKNHEAAVSSRNKIPDNGPFYHGTKADLQVGDLLMPGSNSNYKPELKMNHIYFTA
                     LVNGAGLAAELAKGDGRERVYIVEPTGSFENDPNVTDKKFPGNPTRSYRSLAPLKIVG
                     EVTDWVRLTPEQLQKWKEKLANNKGEIIN"
BASE COUNT          885 a         1058 c         1067 g         1029 t
ORIGIN      
        1 gctttcgttc acccgcaggc gacggtgacg ggaaacgtca tcatcgggaa aaaggtgtac
       61 atcggtccgg gagcggcgat ccgcggcgac tggggcgaga tcattatcga agatggctgc
      121 aacgtgcagg agaactgcac catccacatg tttcccggca tcagcgtcaa gctcatggaa
      181 ggcgcacaca tcggtcacgg cgcagtgata cacggcgcga cgatcgggcg taactgcctc
      241 gtgggaatga attccgtgat catggacgac gtcgtgctcg gcgatgaatg cattgtcggt
      301 gcgctcacgc tcatccgcgc cgatacggtc atcccgccgc gcaagctggt tgtcggcaat
      361 cctgcccgca ttgcgaaaga tgtgagcgat gacatgatcg cctggaagac gaaaggaacg
      421 gagctctacc agcagcttcc cgccgaatgc agggcgacac tgaaggaatg cgaaccgctg
      481 cgcgaagtcc cggtgaaccg gccgaagcag cagaatattt acaggacgtg gggagaaagc
      541 aggaatgatg gatgataaag gaggaatcgc acatcgtaaa ccgtatcccg taaatcacaa
      601 accatggaac gcgtacagat aatcgccatc atcgccagcc tggcgtttct cgttttcatc
      661 tcgagcctga tcgtgcgcgg caggctgcgc gaagaatatg caattatctg gatcgtttgc
      721 tccgcgctgc tgatcgcgtt ctctttctgg cggaaaggac tcgacgtcat gtcgaagctc
      781 atcggggtgt acgatccgcc gaaccttgtt ttcaccggcg ccatcttcgc cgtgatgatc
      841 tacctgctgc atctttccgt ggtcgtttcg cgcctgcagg agcagaacaa aacgctgagc
      901 caggacatcg ccctgctcaa ggaaaaaatg aaagaaaaga aatgagcgtt gaccacggca
      961 aagaagcgct cgtgctcggg aaataccttc tcggcggaaa aattcccgac gaaaaatccg
     1021 tttcgctgta cgttgccgcg catcagaaaa agcccgtttc gctcgacgcg cgcgaagaga
     1081 aaatattcgc cttcctgctc cgccacccgc gctgctgcgg agtgatcgac agcgccctcg
     1141 ccgtttccaa cccccatggc ggcatccgca gaaaaatcat gtacatgtcc gccatcctcg
     1201 aggcaagacc tgcctgggcg gacctgttcc tgccccggga acgaagtttc ttctacaacc
     1261 tttttatttt ctggatagga gcgagggccc tgctgaagat cttcgccggg aaaatactcc
     1321 tggcgttcgt ctgatatttt tcttccgcac cgaattttcg ctgactgctg cgcgataccc
     1381 gcttatcctt ttaccggctc gtatttttcg cggatgaggt aaaggcggtt gtcttccact
     1441 ttgtacagca gcggtgccac ggtgatcttc gggtaacgga ggcgaaggcg tttcgcttcg
     1501 ctgatggaaa aatcgatctc gttaccgatc tcgtgcatcg gttcgtaatg catgaaatgc
     1561 tcttccgcct gcctgcggtc ccacccggct ttctccacca gcccgtcgat aaagagctgc
     1621 ctgcgcgaga cgaggtttac catcccgcag ttgttgtgcc cgatgatcac gatgtggtgg
     1681 atatcgccca cggcgatggc atacgaaact ttgaactcgc tgtaacggag gttcgcgccg
     1741 cctgcgcgga tgatgaacgc gaaattatcg ggaatgcgca ggtgcttgcg gttgtccatg
     1801 cacatgccga tcagcagctt ggcctgcgtg tacggttcga gttcccggcc gagattgtgg
     1861 tattcgagca gcaggccgat gggcgtgttg cggtattccg ggaagatgtc ttcgggtttg
     1921 ttgatttcga ctaagcggtt cgtcatcata ggtgtaccaa tttaaattag cggatgtaag
     1981 acaacgggat caaccgtttg cggtgcaaag atacgaaacg gaaaaatcgg gaaattgatt
     2041 ttatgtcagc tgctttgaca aggcggaaat acccgttcct tcattgcaca tttccggcaa
     2101 acgaccagtc gccgttgctg ttcacgcacg ttcccggcat gctgatgagg tggaggaggt
     2161 ccgactgctg cggaaaaaaa ttcacatgca ggtcgaacgt gtagcggtaa tatttctgcc
     2221 gcggcgtggt gatgatgatc tttttccggg cgatgcgttt cagttctgcg atggctttcc
     2281 gcgaatcggt aatgtgctcg atcgtgtggt tgcagatcac cacgtcgaac gatttgtcgg
     2341 ggaacgggag ctgttcgatc tttccttccg tgaacggaat ggaatcgtct ttcatttcgc
     2401 ggataatatc gcagcccgaa atatccgtgt acccgttttc cttcagcagc ttcagcagga
     2461 aacctttccc gcatcccaca tcgatgatcc ggtcgtgcct gttttccgcc gactgatcga
     2521 gaatgaactg tatcgattcc cggttaaggt cggtttcccg gttcgcgatc gcatcgtaca
     2581 tttcgtaata accggcatat tccctgtccg tcatttttcc ggcgcgctcc ttgaacctca
     2641 tcagcttcga aacgtttttc cccttgtacc agatgaaaaa aagaggatac atgaaaaaac
     2701 ggctgtcccg gatgaccggc ggaaggaact catcgagaac aaagcggatg gagttggtga
     2761 ggttgcggtt catgagccga ttaaagataa gcaaagcgcc cgtctgcttt cggcagatta
     2821 attgataatt tctcccttgt tattcgccag cttttctttc catttctgaa gctgttctgg
     2881 ggttagtctt acccagtcgg ttacctcacc gacaattttc aacggcgcca ggctgcgata
     2941 ggaacgtgta ggattgccag ggaatttttt gtctgtaacg ttcgggtcat tttcaaagct
     3001 gcctgtcggc tcaacgatgt acacgcgttc acgtccgtcc cctttggcga gttctgcggc
     3061 aagcccggcc ccgtttacca atgccgtgaa ataaatgtga ttcatcttga gctcaggttt
     3121 ataattggag ttgctgccgg gcatcagcag atcgccgacc tgcagatccg ctttggtccc
     3181 gtgataaaac gggccattgt caggtatttt gttcctggat gaaacggctg cttcatggtt
     3241 ctttttcgcg ctgtcaaaat cgcccagttc ctcgtagcat ccggcgatat tcaggtacag
     3301 ggaaggaaac gcgcctgcca cagagacatc gttgattttt aaggcagact gcaaagcggt
     3361 ctccagccat tttaatttat ccgtaacatt cgcctggcga cgggccacat aataagcggc
     3421 aagaaatttt tcaaagtcgt ttgtcgcttc gttccaggcc cgtagaaaca gccggcccgc
     3481 ttcttccggg ttgccttttt cttccatgtc catgccttga agacagagct tgacaatgtt
     3541 gttgttcgga ctgaattcca tttttttatt attagttgtg ggctgatgat tgcagtgtcc
     3601 gtttacccgg ccatccatgg atcattgaaa ttgcacacga tgacggccaa cgattccgct
     3661 catgcgcccg tgcgggtttg agcattcaac gttccgggct tgtgcgaatg ttatgtgcgc
     3721 tatttctttc gcctggatat ttttcgtccc atgtaaattg ctccagccag gtttaataag
     3781 atccacgcgt tatagcaaat gtaaattgtt ttttctgtgg cgttcatact tgcccaactc
     3841 cagtatttcc ctttcgagct gtcgtagttg tcatcgtagc gcattagtaa aagcgataag
     3901 atattcatgc ccggcccgta cagccggatc gcctgtaaaa tacctgcatc acctgtcctt
     3961 atgcgggacc ttgcggattt tatatgttgc cttatgcaaa cccgcacctt ccaggagtta
     4021 atgcacccca atccggata
//
LOCUS       DASTSF010000091         8030 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_610817,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000091 DASTSF010000000
VERSION     DASTSF010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 8030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8030
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_610817"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1352)
                     /locus_tag="VFU15_02725"
     CDS             complement(<1..1352)
                     /locus_tag="VFU15_02725"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU4716713.1"
                     /translation="MRISLAFFLLLISAALHSQNENEALLLHQLSAAKDTARIRLLDE
                     LSRYYYEIGAVSLSDSFASVELNEAKKRGDDRLTAIAILRHGQAMDSRGQFDSANAVY
                     DAAIEKLDTNSDRKEYAQALLYKANAFYFKGNTAQAQNLYYRSLVIREAISDSMGMAS
                     SMMGMALVLSDIGQKEKSLDYNLQALGILQRQGELKTVSWLYNNIGTNYADMKRNDEA
                     LAVFAKSLALKKQLNDRYGISTTYNNIGDIFLEEGRYDSALFYFRMGYNIRRKIDDQH
                     ELANSCKLMGMAYLQMNLPDSALPFLDSAQTIGERSGSWDVLKSVYENLALLYAGRGD
                     FRSAFDYEKKFMTARDSVLSEETSRQVNELNARYEDEKKEHQLALKDAEISRGNAFRN
                     LLFGIAALLVVLAVALFTGYRRKRRTSEILAQKNGEIESQKQLVEEKNRDITDSITYA
                     R"
     gene            complement(1464..3395)
                     /locus_tag="VFU15_02730"
     CDS             complement(1464..3395)
                     /locus_tag="VFU15_02730"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU4716714.1"
                     /translation="MHKEPTAQATRDDLLNRLNEARDDAERIDALNALSYDLRLNEIA
                     QSRKYAEEARLLSLELNDKPRLARSLLNLGNAEELSCEYAQALKHLGEAEQLFISLGN
                     DELLFFCNSCTGKVHTMMGDYIRALDYFYRNVHLSEKSGDSRQLVHSLINTGIMHMRL
                     EEYPQAIAIYERSMKISEETGNKSGLAFSYVNLGIVYTSMKEVEKGLGCFLRSAELFR
                     ETKDKVNYSNAIGNIGVAYMELGQTELAEKYVTESFELKEAAGDLNQLSNFLSFARLY
                     CMQKRYPEALENLQRALAVAESTGSRHMFRQVYKSFCDYYEETADTANELKYFKLYHN
                     TDKEIVSEEKNKQMKNLQVKFEVESIQREKEIYRLKNIDLASANEQISKQKEQIEEKN
                     KDITDSISYARRIQRALLAHEGDLRRNLPDYFILYLPKDIVSGDFYWAAETGNQFLLA
                     ACDCTGHGVPGAFMSLLNISFLNECVAEKNVPDPAGILGHVRSRLIRALRDEITGESA
                     RDGMDASIACFDFENRTFSFACANNPVWIIREGTLMEFAPDKMPVGQHDGEMRPFTSQ
                     HFDLEKGDVIYLFTDGYADQFGGPDGKKFKYRQMKDALLSLYGKPMPEQRDELEKTFT
                     AWKGSLEQVDDVLVIGVRI"
     gene            complement(3433..4377)
                     /locus_tag="VFU15_02735"
     CDS             complement(3433..4377)
                     /locus_tag="VFU15_02735"
                     /inference="COORDINATES: protein motif:HMM:NF021554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2279 domain-containing protein"
                     /protein_id="HEU4716715.1"
                     /translation="MRYAAIIILCLFTLHLCAQTDTADGNSSGIRRFACTSAPRVNRK
                     RLLLVAGGETVLAGGSLVALSQLWYKDYPHEKFHFFNDNSEWLQMDKCGHAITSYTLG
                     RYGYNLLRWSDLPEKKCILYGGLTGFAYLTVIEVFDGFSSGWGFSTGDMAANTLGAGL
                     FIGQQLAWHEQRITPKFGYRKSGYAQYRPFLLGADFREQVLKDYNGQTYWLSVNVASF
                     LGDGTKFPGWLNIAVGYGANGMTGGKSNPFMQNAAGNTICFPRYRQLFLSPDIDLTRI
                     PVRSKWLGRIFKTFGFLKIPAPGIELGRGKISGRWLLF"
     gene            complement(4457..5152)
                     /locus_tag="VFU15_02740"
     CDS             complement(4457..5152)
                     /locus_tag="VFU15_02740"
                     /inference="COORDINATES: protein motif:HMM:NF019325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEU4716716.1"
                     /translation="MTVLLCGGMKLAAQDIAPGINYSYDPPGSDGVIHNITVDVVNND
                     NTGAGSFDVAMYLYDQSSGNHWVIGTTNIPSLSGNSLITISSWDIDINNTPGIPAGTY
                     RLGIWVDSNDDISETDENNNTGLLAGNISYNPSASGIASAVVAASSVTCFPNPANVSA
                     TFTYSLTQNTNVSVKLYDITGNLVSTLSDNEQSAAGDHQLTIDTSSLPDGVYFITLST
                     GEESVVRRVVVSH"
     gene            5399..6640
                     /locus_tag="VFU15_02745"
     CDS             5399..6640
                     /locus_tag="VFU15_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009507723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU4716717.1"
                     /translation="MTRIDPKLRRQILLIILVAGMGYFVDIYDLILFLFVKKSSLAEL
                     NIPETEYRSLLNYQMVGMLLGGIVWGILGDKKGRLSTLFFTILIYSLANIANGFVQDL
                     EQYRILRFIAGFGLAGELGIGITLVSEIMSKESRGYGTAIVAGIGIAGAALGYFVTHY
                     YGWRMAFWTGGGLGLALLVLRVSIHESTMFAAAKKSELKRGNFLELFTSRAKLKKYLF
                     CILIGVPVWYVIGIIIAGSEKFALNVFHIDGTEGVKDIGAKSVFYHYIGASIGSFITG
                     FMSQWLRSRKKALILSLIGLVVILAAFFCSYGVSLSTYYFVIFLLGIVQGYWAIFVTI
                     SSEQFGTNLRATVATSVPNIVRGATVLMTLSYASLGTAEEGSTKGAIIVGAVVMALAF
                     WAAFSLKETYGKELDYLEPAE"
     gene            6637..6993
                     /locus_tag="VFU15_02750"
     CDS             6637..6993
                     /locus_tag="VFU15_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019946808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEU4716718.1"
                     /translation="MKNVVIGASENPERYSNRAVRSLKSHGKEVVAVGIHEGEIDGIK
                     IRTDRPQLTDVDTVTMYVGPKNQPAWYDYILSLKPKRIIFNPGAENPEFEKIAKENGI
                     EVVEACTLVMLSIGNY"
     gene            complement(7341..7775)
                     /locus_tag="VFU15_02755"
     CDS             complement(7341..7775)
                     /locus_tag="VFU15_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016876391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU4716719.1"
                     /translation="MIIRIASTDEEIQKCFPAIHALRPFLKEDEFVSRIRRLMKDGFI
                     LAYVEMEDGSVPAASGFRIEEMLHRGKSIYIDELSTLESARSKGLGGMLIDYIIDLAK
                     KEDCASVHLDSGVQRFAAHRFYLKKKFDIVSHHFALHLKSIE"
     gene            complement(7806..>8030)
                     /locus_tag="VFU15_02760"
     CDS             complement(7806..>8030)
                     /locus_tag="VFU15_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006990347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEU4716720.1"
                     /translation="SNEASERLTCSYAGDDMEIGFNSRFLAEMLTNIGSDEAVIEMSA
                     PNRAGILLPAVKATEAEDTLMLVMPVMLNN"
BASE COUNT         1713 a         2131 c         2173 g         2013 t
ORIGIN      
        1 cgcgcatagg tgatgctgtc ggtgatgtcg cggttctttt cttccacgag ctgtttctgg
       61 ctttcgatct ccccgttttt ctgcgcaagg atttccgacg tgcgccgctt gcggcggtaa
      121 ccggtgaaga gcgcaaccgc aagcaccacg agcagggcgg cgatcccgaa aagcaggttg
      181 cggaacgcat tgccgcgcga gatctccgcg tctttcagcg cgagctggtg ctcttttttt
      241 tcatcctcgt agcgtgcgtt cagctcattc acctgcctgg atgtctcttc gctgaggacg
      301 gaatcgcgag cggtcatgaa ctttttctca taatcgaacg cgctcctgaa atctccccgc
      361 ccggcgtaga gcagcgcaag gttttcgtag acgcttttca gcacatccca cgatccgctt
      421 ctttcaccga tggtctgcgc tgaatcgagg aagggcagcg cgctgtcggg caggttcatc
      481 tgcaggtaag ccattcccat cagcttgcag gaattcgcca gctcatgctg atcgtcgatc
      541 ttccggcgta tgttgtaccc catccggaaa tagaaaagcg cagaatcgta acggccttct
      601 tcaaggaaaa tatcgccgat gttgttatac gtggtggata tgccgtaccg atcgttcagt
      661 tgctttttca gcgccagcga tttggcaaaa acagccagcg cctcgtcgtt tcgtttcatg
      721 tccgcgtaat tcgtcccgat gttgttgtac agccatgaaa cggttttcag ttccccctgc
      781 cgctggagga tgccgagcgc ctggagattg taatcaagcg atttttcctt ctgtccgatg
      841 tcactcagca cgagcgccat tcccatcatc gaggaagcca ttcccatgct gtcgctgatc
      901 gcttcacgga tgacgagcga gcggtaatac aggttttgcg cctgcgcagt gttgcctttg
      961 aaataaaatg cgttcgcttt gtagagcagc gcctgcgcat attctttccg gtcggaattg
     1021 gtgtccagct tttcaatcgc agcgtcataa actgcattcg cggaatcgaa ctgcccgcgt
     1081 gaatccatcg cctgcccgtg acgcaggatc gcgatcgcgg taaggcggtc gtccccgcgc
     1141 ttttttgctt cgttcagctc gaccgatgca aaagagtcag aaagggagac agcgccgatc
     1201 tcgtaataat acctgctcag ctcatcgagc aggcggatgc gggctgtgtc cttcgcagcg
     1261 gaaagctggt gcagcagcaa tgcttcattt tcattttgcg aatgcaatgc agccgaaatc
     1321 agcagcagga aaaaggcaag ggagattctc acatgcgggt cttgagcatt ctaaaatacg
     1381 gaatgaaaat aagaccacga aaagaagcag cacgaaagcg ggaacgcgga agggtttcgc
     1441 ggccccggca ttcaccggct tcgtcagatc ctcacgccga tgaccagtac gtcatccacc
     1501 tgctcgaggg atcctttcca ggcggtgaat gttttttcca gctcatcgcg ttgttccggc
     1561 atcggtttcc cgtaaagaga aagcagcgca tctttcatct ggcggtattt gaatttcttt
     1621 ccgtcaggtc ccccgaactg atcggcatag ccgtccgtga aaaggtaaat gacgtcgcct
     1681 ttttcaagat caaaatgctg cgaagtgaac ggccgcattt caccgtcgtg ctgaccgaca
     1741 ggcattttat cgggcgcaaa ttccatgagc gttccttcgc gtatgatcca gaccgggtta
     1801 ttggcgcagg cgaagctgaa cgtcctgttt tcgaaatcga agcaggcgat ggaagcatcc
     1861 attccgtcgc gtgcgctttc cccggtaatt tcatcacgca gtgcacgtat gagacggctc
     1921 cgcacgtggc cgagaatacc cgcaggatcg gggacattct tttccgcgac gcattcgttg
     1981 aggaacgaaa tgttcagcag cgacatgaaa gcgcccggca cgccgtgacc tgtgcaatcg
     2041 cacgcggcga ggaggaattg gttgcctgtt tccgccgccc agtaaaaatc accgctgacg
     2101 atatctttcg gaaggtaaag gataaaatag tccgggagat ttctgcgcag gtccccctcg
     2161 tgggccagca gcgcacgctg tatccggcgc gcatagctga tggagtcggt gatgtccttg
     2221 ttcttttcct cgatctgctc tttctgtttc gatatctgct cattggcgct ggcaagatcg
     2281 atgttcttca ggcggtagat ctctttttcg cgttgaatgg attccacttc gaatttcacc
     2341 tggaggtttt tcatctgctt gttcttctcc tcgctgacga tctctttatc ggtgttgtga
     2401 tacagcttga aatacttcag ctcgttcgcc gtatcggcgg tctcttcgta ataatcgcag
     2461 aacgatttgt acacctggcg gaacatgtgc ctgctgccgg tcgattcggc taccgcaagt
     2521 gctcgctgaa gattttccag cgcttccggg tagcgtttct gcatgcagta caaccgggca
     2581 aaagaaagga aattgctcag ctggttgagg tcgccggccg cctctttcag ctcaaagctt
     2641 tcagtgacgt atttctccgc aagctccgtt tgtcccagct ccatgtaagc aacgccgata
     2701 ttcccgatcg cgttggaata gttcaccttg tctttcgttt cccggaacag ctccgcggag
     2761 cgaaggaagc agccgagtcc tttctccact tctttcatgg acgtataaac aatgccaagg
     2821 ttgacgtagg aaaaggcgag accgctttta tttcccgttt cttcgctgat cttcatcgag
     2881 cgttcgtaga tcgcgatcgc ctggggatat tcttcgaggc gcatgtgcat gattcccgta
     2941 ttgatgaggg aatggaccag ctgcctgctg tcaccgcttt tttccgaaag atgaacgttg
     3001 cggtagaaat aatcgagcgc gcggatgtaa tcgcccatca tcgtgtgcac ttttccggtg
     3061 cagctgttgc agaaaaaaag aagctcgtcg ttgccgaggg aaatgaacag ctgttcggct
     3121 tcacccaggt gcttgagcgc ctgtgcatat tcgcagctga gctcttctgc gttcccgaga
     3181 ttgagcaggc tccgcgccag cctcggcttg tcgttcagct cgagtgaaag caggcgcgct
     3241 tcttccgcgt atttccggct ctgcgcaatt tcattcagcc gcaggtcgta agacaatgca
     3301 ttcagcgcgt cgatccgctc cgcatcgtcg cgcgcttcat tgagcctgtt cagcaggtcg
     3361 tcccttgtcg cctgtgctgt gggttctttg tgcatgggga ttttaaggta atcatctttt
     3421 ccggaaaccc gctcagaaca gcagccacct gccgcttatt tttccgcgac ccagttcgat
     3481 ccccggtgcg ggaattttca ggaacccgaa tgttttgaat attctgccca gccatttcga
     3541 tcgcacggga atccgtgtaa ggtcgatgtc cggtgaaaga aataactggc ggtagcgcgg
     3601 gaaacagatc gtgttcccgg cggcgttctg catgaacggg ttggactttc cgcctgtcat
     3661 cccgttcgcg ccgtagccga cggcgatgtt cagccagccc gggaattttg ttccatcgcc
     3721 gaggaaagaa gcgacgttca ccgagagcca gtaagtttga ccgttgtaat ctttcagcac
     3781 ctgttcccgg aaatcagcgc cgagcaggaa agggcggtat tgcgcataac cgcttttcct
     3841 gtaaccgaac ttcggcgtga tgcgctgttc gtgccatgca agctgctgcc cgatgaacag
     3901 tcccgccccg agcgtgttgg ccgccatgtc gccggtggaa aatccccagc cggaagaaaa
     3961 tccgtcgaac acttcgatca cggtgaggta agcaaaaccc gtaagccccc cgtacaggat
     4021 gcactttttt tccgggagat cgctccagcg gagcaggttg taaccgtatc tcccgagcgt
     4081 gtacgaggtg atcgcgtgac cgcatttgtc catctgcagc cattcggaat tgtcgttgaa
     4141 gaaatgaaat ttttcgtgcg ggtaatcttt gtaccagagc tggctcagcg caacgagaga
     4201 accgccggcc agcacagttt ctccgccggc caccagcagc aggcgtttcc tgttaacacg
     4261 cggagccgaa gtacatgcga atcgccgtat cccgctggaa tttccatccg cggtatctgt
     4321 ctgtgcgcac agatgcaatg taaaaaggca gagaatgatg atcgccgcgt agcgcattgg
     4381 gtaaagatac ttccttccgg aaacaaaaaa tccccggcga ggtcagtcac cggggatgaa
     4441 acgccacgcg caaaacttaa tgtgacacaa ccacgcggcg gactacactt tcttctcctg
     4501 tcgaaagtgt aatgaagtaa acgccgtccg gaagcgacga tgtgtcgatc gtcagctggt
     4561 gatctcctgc ggccgactgt tcgttatcgc tcagcgtgct cacgagattt cccgtgatgt
     4621 catacagctt cacggaaacg ttcgtattct gcgtaagcga ataggtgaat gttgccgaaa
     4681 cattggccgg gttcgggaag cacgttacgg aagaagccgc cacaactgct gaagcgatac
     4741 cgcttgcgct cgggttgtag ctgatgttcc cggccagcag tcccgtgttg ttgttctcgt
     4801 ccgtttcgct gatgtcgtcg ttcgagtcca cccagatgcc gagacgataa gtgcctgcgg
     4861 gaatgcccgg ggtgttgttg atgtcgatgt cccagctgga aatggtgata agcgaatttc
     4921 ccgaaaggct gggaatgttt gtcgtgccga tcacccaatg attcccggag ctctggtcgt
     4981 agagatacat cgccacgtcg aacgaaccgg cgccggtgtt gtcgttgttc accacgtcga
     5041 ccgtgatgtt gtggatgacg ccgtcggatc cgggaggatc gtagctgtag ttgatgccgg
     5101 gagcgatgtc ctgcgccgca agtttcattc cgccgcagag cagcactgtc ataaggagta
     5161 aggattttgt tttcatgatc tgtttttgtt tttggtgatg taaaggaaca gccataatcc
     5221 tgccgcggtc agcggtgcat tcccgcgttc gttcatgtac gcacacaaaa agacgcagga
     5281 tggcttcaat cgccctgcag gaagcgggtt gaaaaaaatg tttgtgaatt cacacggaaa
     5341 tgaaattgcg ccggccggaa ctcaatttcc ccgatctgtt atctttactg aactgtttat
     5401 gacacgtatt gatccgaaac tccgccggca gatcctgctg attattcttg ttgcaggaat
     5461 gggttacttc gtcgatatct acgatctcat tcttttcctt ttcgtgaaga agtcgagcct
     5521 ggccgaactg aacatcccgg aaacggaata ccgctcactc ctgaattacc agatggtggg
     5581 aatgctgctg ggcggtatcg tctggggaat tctcggtgat aaaaaaggaa gactctcgac
     5641 gctctttttc accatactca tttattcgct ggcgaacatt gccaacgggt tcgtgcagga
     5701 cctggagcaa taccgcatcc tgcgcttcat tgcggggttc gggctggccg gcgagctcgg
     5761 catcggcatc acgcttgttt ccgagatcat gtcgaaggaa tcacgcgggt acggaacagc
     5821 gatcgtggcc ggcatcggca ttgcgggtgc ggcgctcggc tatttcgtga cacattatta
     5881 cggctggcgc atggcgttct ggaccggcgg cggactcggg ctggcgcttc tcgtgctgcg
     5941 cgtctccatc catgaatcca cgatgttcgc agcggcaaaa aaatcggaac tgaagcgcgg
     6001 gaatttcctt gagctgttca cgagccgggc caagctgaaa aaatatcttt tctgcattct
     6061 tatcggtgtg ccggtgtggt acgtgatcgg catcatcatc gccggctcgg aaaagtttgc
     6121 gctcaatgtt tttcacatcg acggaacgga aggcgtgaag gacatcggcg cgaaatccgt
     6181 tttctaccat tacatcggcg cttccatcgg cagctttatt accggcttca tgagccagtg
     6241 gctgaggtcg cggaaaaaag cgctcattct ttcgctcatc gggctcgtcg ttatccttgc
     6301 cgcgttcttc tgctcctacg gtgtttcgct gagcacgtat tatttcgtca ttttcctgct
     6361 cggcatcgtg cagggatact gggcgatctt cgtcaccatc tcgtcggagc agttcggcac
     6421 caacctgcgc gccaccgtgg cgaccagcgt tccgaacatt gtgcgcggcg ctaccgtact
     6481 gatgacgctg agctacgctt cgctgggcac agctgaagaa ggctcgacga aaggtgcgat
     6541 catcgtgggc gcagttgtca tggcgcttgc gttctgggct gcgttcagcc tgaaggaaac
     6601 ctatggcaaa gagctggatt atttagaacc tgcagaatga aaaacgtcgt catcggtgca
     6661 tcggaaaacc cggaacgcta cagcaaccgc gcggtgcgtt ccctgaaaag tcacgggaaa
     6721 gaagtggtcg ccgtcggcat tcacgaaggc gaaatcgacg ggataaagat ccgcacggat
     6781 cgcccgcagc ttacggatgt ggatacagta acgatgtacg tcggacctaa aaaccagccg
     6841 gcgtggtacg attatatttt atcgctgaaa ccgaaacgca tcatcttcaa cccgggagct
     6901 gaaaaccccg aattcgaaaa gatcgcaaag gaaaacggga tcgaagtggt ggaagcctgc
     6961 acgctcgtga tgctcagtat cggtaattat tgaaatcgga aatcagcagc gagacaaaac
     7021 ttcatctcac gcctgatctg taatgtttaa gttcccggtt tcggatttcg cgtttcgggt
     7081 tgtcttcgtt ttccttcgtg gtttcaactt ttcaactttg aaactgaatt accttcgtga
     7141 aaacgctttg tgcactttgt gaaaacgctt tgtgcacttc gtggtaaaaa cgatttcagt
     7201 ttatcacagg tcattcgtcg ctactttcca acttttcaac gttaaaactg aattgcattc
     7261 gtgcccttcg tgaaaacgct ttgtgttctt cgtggttaaa atagtttcgg gtttcgggtt
     7321 tgtcccgcgt caaatgatca ttactcaata ctcttgaggt gaagcgcaaa atgatggctc
     7381 acgatatcaa atttcttttt caggtaaaaa cggtgcgccg cgaaacgctg cacgccagaa
     7441 tcgaggtgaa cgctggcaca atcttctttt ttcgcaaggt cgatgatgta atcgatcagc
     7501 atgccgccga gtcctttgct gcgcgccgat tcgagcgtgc tcagctcgtc gatataaatg
     7561 cttttgcccc ggtgcagcat ctcttcaatg cggaaaccgc tcgcggcggg aacgcttccg
     7621 tcttccattt ccacgtacgc gaggatgaag ccgtccttca tcaggcggcg gatgcgtgaa
     7681 acgaattcat cttccttcag gaacgggcga agcgcgtgaa tcgcgggaaa acacttctgt
     7741 atctcttcgt ctgtggaagc gatgcggatg atcatggcgt gaatttcacc ggttgccggc
     7801 ggccatcagt tattcagcat cacgggcatc accagcatga gcgtgtcttc cgcttccgtg
     7861 gctttcacag cgggaagaag aatgcccgcg cggttgggcg ccgacatttc gatcaccgct
     7921 tcatcgctgc cgatgttggt gagcatttcc gccaggaaac gtgaattgaa gccgatctcc
     7981 atgtcgtcgc cggcgtaaga gcaggtgaga cgctccgacg cttcgttcga
//
LOCUS       DASTSF010000092         9840 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_164597,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000092 DASTSF010000000
VERSION     DASTSF010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 9840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9840
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164597"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..920)
                     /gene="dnaX"
                     /locus_tag="VFU15_02765"
     CDS             complement(<1..920)
                     /gene="dnaX"
                     /locus_tag="VFU15_02765"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017257425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEU4716721.1"
                     /translation="MDNFIVSARKYRPATFNTVVGQSSITTTLKNAIKNNHLAQAFLF
                     CGPRGVGKTTCARILAKTINCSDLTPETEACNKCESCLSFNSGQSFNVYELDAASNNS
                     VEDIRSLVDQVRIPPQMGQYKVYIIDEVHMLSQAAFNAFLKTLEEPPKYAIFILATTE
                     RHKILPTILSRCQIFNFNRIGVEDIAGHLAYIAKNENVEADPDALHVIAQKADGALRD
                     ACSIFDQVVSFAGNHLTYKAVIENLNVLDADYYFRITDDLLAENIPSTLLVFDEILSN
                     GFDGHNFIAGLAEHFRNLLVSKDQATLQLL"
     gene            complement(1064..3124)
                     /locus_tag="VFU15_02770"
     CDS             complement(1064..3124)
                     /locus_tag="VFU15_02770"
                     /inference="COORDINATES: protein motif:HMM:NF022985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Omp28-related outer membrane protein"
                     /protein_id="HEU4716722.1"
                     /translation="MKKITITAVLAAGLLAGATHAQAPKFVLFEHFTQASCAPCAAQN
                     PGFQNTILTPNPDKVRHIAVHTSWPGVDPMYNYNTALSAARVTYYNITGVPTVVMLGN
                     QKTGQPGAFTQSDVDNAFSAGSPIKITVNDVDNGTTHDVTVNVLTVGTVPSGTYKLRA
                     AIVEDPITYATPPGTNGETTFPDVLRQMLPSASGDPYTPAAIGNTVTFNYTYTEDAAW
                     VMPNIKVIAFVQNETTKEILQCGTMNDPVINYTLSTPAVEIMHGTSANASSFSLNSMN
                     TGSGSEDFVYTLTTDIPGDWTANFSVNSTTYSGNATITTAAAATNNIMINITPGTTPY
                     VGRATLTVASASNPSLPVLTKNVYVISNVTDLIVNNTGGIGDGVTAGNASNWDSCYVN
                     GMIFANEPGWAKTTSDICTKAIQDNAFQGIHNVYYNVGWTFPSLTDNFCTQMTSFLNA
                     GGCLFISGQDIAWETWDTANSPYWTSTTENFFSTYFGATFSADGSTANSQLTAVTSDP
                     VFGTVPNSTIGNFYGGNYFYPDEINAANGGTAVFKYNNGTKTAGTRNFNGTWKVVYIS
                     VGIEMLTDYNAKNEILKWSHDWFYGLLSTNDFDHNMNSLSIGQNYPNPSNLSTIIPLD
                     NVKNDMTLQVVDLQGRVVMSQAVAAGATQAEINTADLAAGTYMYRLMNGQDVTETKRL
                     QVVH"
     gene            complement(3209..3718)
                     /gene="idi"
                     /locus_tag="VFU15_02775"
     CDS             complement(3209..3718)
                     /gene="idi"
                     /locus_tag="VFU15_02775"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010181156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HEU4716723.1"
                     /translation="MEEIILVDSHDREKGKMEKLQAHREGWLHRAVSVFVFNSKGELL
                     IQRRAAGKYHSPALWTNTCCGHPRPGEQNADAAARRLREEMGMNVPLEEIFSFVYKVK
                     LADDLFEHEFDHVFTGRSDELPRPDPDEVMEWKYASLEELMKDTDARQQDYTIWFLMI
                     FREAFKWLK"
     gene            3893..5236
                     /gene="purB"
                     /locus_tag="VFU15_02780"
     CDS             3893..5236
                     /gene="purB"
                     /locus_tag="VFU15_02780"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEU4716724.1"
                     /translation="MSIDSLWAVSPVDGRYRKQVEELSNYFSEGALIRYRLVVETEYF
                     IALCRLPLPQLKGIGEKTFGELRRDIYENFSPADAQRVKEIEKTTNHDVKAVEYVMKE
                     KFDRLKLDAHKEFVHFGLTSQDINNTATPMMIRDAWKNTLFPLLENVLHSLEKMAAEW
                     ENIPMLAHTHGQPASPTRLGKELRVFTERLKEQLRLLEMVPFSAKFGGATGNFNAHHV
                     AYPSVDWKKFGDDFIASLGLKRSHPTTQIEHYDNLAAFFDGLKRINTILIDLSRDFWQ
                     YISMNYFRQKVKEGEVGSSAMPHKVNPIDFENAEGNLGFANAIFEHLSAKLPVSRLQR
                     DLTDSTVLRNAGVPFAHTIIALKSLQKGISKLLLNEDALNKDLEQNWAVVAEAIQTIL
                     RREGYPKPYEALKDLTRGNASVTKDSMHAFIDRLSVSDEIKKELKKITPQNYTGI"
     gene            5550..6425
                     /locus_tag="VFU15_02785"
     CDS             5550..6425
                     /locus_tag="VFU15_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020595047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU4716725.1"
                     /translation="MFVSGFTIVRNAVKFDYPVAEAIRSILPLCDEVVVAVGKSEDET
                     LQLIRSIGDPKIRIVETVWNDALREGGRVLADETNKALDAVSEKADWCFYIQGDEVIH
                     EKYHPAIREAMLKWKDDKNVEGLLFDYRHFYGSYDYVGDSRKWYRHEIRVVRNDRNIR
                     SYRDAQGFRKKDNSKLRVKKSGAEVFHYGWVKPPQAQQAKQESFHKMWHDDEWMKKNI
                     PVAEEFDYSQIDSLAKFSGTHPAVMKERTERMNWKFSFDPTKKKWGFKMRLLMWTEKT
                     TGWRPGEYKNYILLK"
     gene            complement(6427..6939)
                     /locus_tag="VFU15_02790"
     CDS             complement(6427..6939)
                     /locus_tag="VFU15_02790"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEU4716726.1"
                     /translation="MMKDAKEFLLAQARYNLWADKRIADVFSRMSEESFLRETKSSFP
                     TIRKTAFHIWDAEHIWLQRLKGVSPADWNSRSLPADADPRMFVKGAEDFLAFLEMQDE
                     SFFTASTSFKDMKGNPYTMQNAQMIFHCMNHGTFHRGQLVTMIRESGWTEKLPSTDMI
                     TYFREVNGMP"
     gene            complement(6970..7692)
                     /locus_tag="VFU15_02795"
     CDS             complement(6970..7692)
                     /locus_tag="VFU15_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEU4716727.1"
                     /translation="MIVDLFIPCFVDQLYPDTALNMIKVLERVGCGVNYNHEQTCCGQ
                     PAFNAGYWDHCKEVGEKFIREFQSDRYIVSPSSSCPGFVKNYYGEMFHNTALHNQFRQ
                     VQKNMYEFSDFLVNILKITDLGAKMEGVAVFHDSCSGLREYGIHREPRVLLSKVRGLE
                     VREMEKSDVCCGFGGMFSVKYENIAVGMAEQKLKTAEDAGAEYIISTDLSCLLHLDGF
                     IKKQKKNIKVMHLVDVLVQGWE"
     gene            complement(7783..9378)
                     /locus_tag="VFU15_02800"
     CDS             complement(7783..9378)
                     /locus_tag="VFU15_02800"
                     /inference="COORDINATES: protein motif:HMM:NF023128.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU4716728.1"
                     /translation="MLKKGDPKVIRAWTFYDWANSVYPLVISSAIFPIYYEAVVKKDL
                     GGRIAIGGASFNSTEFYSYIAAISYIIVALSSPVLSGIADFSGSKKKFLRFFCFLGAG
                     CCMLLGFFSTQHILLSMLALMFASVGYWGSLVFYNAYLPEIAPPEDQDKVSARGFGMG
                     YFGSSLLLILILVLAVFTHVIPHIRYSFFLVGLWWIGFAQITFRRLPKGKAHENKTNE
                     NIVTKGYRELRLVFRDILTRKQLRRFLGSYFMYNMGVQTVMIMATLFATEAVDWQSPG
                     DVQSAIVQVQADAKAGKITMVNGAESITPEQVRSQIDAVMKLNRKLSDTLSAGSRTAA
                     IRKNIETVQQDMRGTVAADDVRLAPGYADYLADVYQRAETLAEGHESQSLIISILLIQ
                     FLGMAGAFLMSRLSRVIGNLKTIGIVIFLWILICYGTFQFVYKPVQFYIVAASVGLVM
                     GGIQSMSRSTYSKMLPETEDHASYFSFFDVSEKIGLAIGTMTFGLIAGITGGIRQSVL
                     SLIVIFVLGFLLLLRVPKSQNVR"
     gene            complement(9499..>9840)
                     /locus_tag="VFU15_02805"
     CDS             complement(9499..>9840)
                     /locus_tag="VFU15_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016479898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding domain-containing protein"
                     /protein_id="HEU4716729.1"
                     /translation="DTLNTESPALPPQRQQQLYENVAADYAELSKGARYKKMKEDPWF
                     NALQVKFAYAVTCHKAQGGQWKAVFVEQGYLTEEMINTEFLRWLYTAITRTTEKVFLV
                     GFNKKFFGETD"
BASE COUNT         2280 a         2607 c         2634 g         2319 t
ORIGIN      
        1 tcgagcagct gcagcgtcgc ctggtctttc gaaacgagga ggttccggaa atgttcggca
       61 agtcctgcga tgaaattgtg cccgtcgaac ccgttcgaaa gaatttcatc gaatacaagc
      121 agcgtggaag gaatattttc cgcgagcagg tcgtccgtga tgcggaaata ataatccgca
      181 tcgagcacgt tgaggttctc gatgacggct ttgtacgtaa ggtgatttcc cgcgaagctc
      241 accacctggt cgaagatgga gcaggcatcc cgcaatgcgc cgtcggcttt ctgcgcgatc
      301 acgtgcagcg catcgggatc ggcttccacg ttctcgtttt tcgcgatata cgcaaggtgt
      361 cccgcgatat cttccacgcc gatgcggttg aaattgaaga tctggcaacg ggaaaggatc
      421 gtcggcagga tcttatggcg ctcggtggtg gcgagaatga agatggcgta tttcggcggt
      481 tcttccagcg ttttcaggaa agcattgaac gccgcctgcg aaagcatgtg cacctcatcg
      541 atgatgtaga ccttgtattg tcccatctgc ggcggaatgc gcacctggtc gacaagactg
      601 cggatatctt ccaccgaatt gttcgacgcg gcatccagtt cgtatacatt gaacgactgg
      661 ccggaattga aggaaagaca ggattcgcat ttattgcatg cttccgtttc cggtgtaagg
      721 tccgaacagt tgatcgtctt cgccaggata cgcgcgcagg ttgtttttcc cacgccgcgc
      781 ggaccgcaga acaggaacgc ctgtgccagg tggttgttct ttatcgcatt cttcagcgtg
      841 gtggtaatgg acgactgccc caccacggtg ttgaacgtgg ccggccggta cttgcgcgct
      901 gaaacgatga aattgtccat tggggctgta aaaataaaga tagcgccgcg ttctgcgaag
      961 cgaagcggaa agacttacta tgttttttca acagcgtcct gcctcagaaa aaaagacgcc
     1021 acccttgcgg atggcgtcct tttcaattct ttcagcagaa aaatcaatgc actacctgca
     1081 ggcgtttcgt ttccgtaaca tcctgcccgt tcatgaggcg gtacatgtac gttcctgctg
     1141 cgagatcagc tgtattgatc tccgcctgtg ttgcacctgc tgcgacagcc tgcgacatca
     1201 ccacacgtcc ctgcagatcg acaacctgga gtgtcatgtc gttcttcaca ttgtcgagcg
     1261 ggatgatggt agacaggttg ctcgggttcg ggtagttctg cccgatgctg aggctgttca
     1321 tgttgtggtc gaaatcattc gtgctgagaa gaccgtagaa ccagtcgtgc gaccatttca
     1381 ggatctcgtt cttcgcattg taatccgtca gcatttcgat acccacggag atgtacacca
     1441 ctttccacgt tccgttgaaa ttgcgtgttc cggcggtctt ggttccgttg ttgtatttga
     1501 acacggccgt tccgccgttg gcggcgttga tctcatccgg gtagaaatag tttccgccgt
     1561 agaaatttcc gatcgtgctg ttcggtaccg ttccgaaaac gggatcgctc gtcacggcgg
     1621 tcagctgcga attggctgtt gatccgtctg cggagaacgt cgcgccgaaa taggtcgaga
     1681 agaaattttc agtggtagac gtccagtacg ggctgttcgc cgtatcccag gtttcccagg
     1741 cgatatcctg cccggagatg aagaggcagc cgcctgcgtt gaggaagctg gtcatctgtg
     1801 tgcagaaatt gtcggtgagt gaagggaaag tccagccaac gttgtaatac acgttgtgaa
     1861 ttccctggaa tgcgttgtcc tggatcgctt tcgtgcagat atcgctggtg gttttcgccc
     1921 agcccggttc gttcgcgaag atcattccgt tcacatagca ggaatcccag ttggaagcgt
     1981 tcccggctgt aacaccgtcg ccgatgccgc ccgtgttgtt cacgataaga tccgttacgt
     2041 tcgagatcac gtacacattt ttcgtgagca caggaagcga aggattgctt gcagaagcca
     2101 cggtgagcgt agcgcgtccc acgtaaggcg tcgtgcccgg cgtgatgttg atcatgatgt
     2161 tgttcgttgc cgcagcagcg gtcgtgatcg tcgcgttacc ggaatacgtg gtgctgttca
     2221 ccgagaaatt ggcagtccag tcgcccggga tgtctgtcgt gagcgtgtaa acgaaatctt
     2281 cggaaccgct gcccgtgttc atcgagttga gcgagaaaga agaagcgttc gcggaagttc
     2341 cgtgcatgat ctcaacggca ggcgtggaaa gcgtgtagtt gatgaccgga tcgttcatcg
     2401 ttccgcactg gaggatttct ttcgtcgttt cattctgaac gaaagcgatc accttgatgt
     2461 tcggcatcac ccatgcagcg tcttcggtgt acgtgtagtt gaacgtcacc gtgttgccga
     2521 tggcagccgg tgtgtacgga tcgcctgaag cgctcggaag catctggcgg agcacatcag
     2581 ggaacgtggt ctccccgttc gttcccggag gcgtcgcgta cgtgatcgga tcttcgacga
     2641 tcgccgcgcg gagcttgtac gttccggaag gaaccgttcc gacggtgagc acgtttaccg
     2701 tcacgtcgtg cgtggtcccg ttatcgacgt cattgacggt gatcttgatc gggcttcctg
     2761 ccgagaaagc attatcgacg tcggactggg tgaaagcgcc cggctggcct gttttctggt
     2821 tgccgagcat caccacggtc ggaacaccgg tgatgttgta atacgtgacg cgtgcagcgc
     2881 tgagcgccgt gttgtagttg tacatgggat cgacacccgg ccatgaggtg tgcacggcaa
     2941 tgtggcgaac cttgtcagga ttgggcgtca ggatggtatt ctgaaacccc ggattctgcg
     3001 cagcgcaggg agcgcagctg gcctgcgtga aatgttcaaa aagaacaaat ttcggtgcct
     3061 gggcatgggt cgctcctgcg aggagacctg cggcaagcac agcagtaatc gtaatttttt
     3121 tcatgatgtt ggtttttgtt gagcctggaa gttgtttgag aataacaaat ataaaaaatg
     3181 cacggtgcgg tacaagccca aaaccgtttt atttcagcca tttgaaggcc tcgcggaaga
     3241 tcatcaggaa ccagatcgtg taatcctgct ggcgggcgtc cgtatctttc atcagttcct
     3301 ccagcgaagc atatttccat tccatcactt catccggatc aggccgggga agctcgtccg
     3361 atctgccggt aaaaacgtga tcgaactcgt gctcgaacag gtcgtcggcg agcttcactt
     3421 tgtaaacgaa gctgaaaatt tcttcgagcg gaacattcat tcccatttct tcccgcaggc
     3481 ggcgggccgc ggcgtccgca ttctgctcac ccggccgcgg gtggccgcag caggtattgg
     3541 tccagagcgc gggagaatgg tatttgcccg ctgcacggcg ctggatcagc aattctcctt
     3601 tggaattgaa cacgaaaacg gaaaccgcgc ggtgaagcca cccttccctg tgcgcctgca
     3661 gcttttccat ttttcctttt tcgcgatcat gggaatcgac gaggatgatc tcttccataa
     3721 aacagttcgt gttcggcggg ggaatttcca aataaatgaa acgcttaccc gtattatccc
     3781 ggataacttt tccacaacag cgctgtaaaa acctctgcag aatcggtttg ccgcacgccc
     3841 ggcgtccacg gagaacagcc attcttgcta acttcgcgtt ttccgtttta ctatgagcat
     3901 tgattctctc tgggccgttt ctcctgtcga cggtcgttac cgcaaacagg tggaagagct
     3961 ttcgaactac ttttccgagg gcgcgctgat ccgctaccgc ctggtcgtgg agaccgaata
     4021 tttcatcgcg ctctgccgtt tgcccctgcc ccagctgaaa ggcatcggtg aaaaaacctt
     4081 cggggagctt cgccgcgaca tctacgaaaa tttcagtcct gccgatgcgc agcgcgttaa
     4141 ggagatcgag aaaacgacca accacgacgt gaaggcggtg gaatatgtga tgaaggaaaa
     4201 gttcgaccgg ctgaagctcg atgcgcataa ggagttcgtt catttcgggc tcacctcgca
     4261 ggacatcaac aacacggcca cgccgatgat gatccgcgat gcgtggaaga atacgctttt
     4321 cccgctgctg gagaatgtgc tgcacagcct cgaaaaaatg gccgcggaat gggaaaacat
     4381 ccccatgctg gcgcacacgc acggacaacc ggcgtcgccc acgcgcctgg gaaaagagct
     4441 ccgcgtgttc accgaacgcc tgaaagagca gctccgcctg ctggaaatgg ttccgttcag
     4501 cgccaagttc ggcggtgcga cgggaaattt caatgcgcac cacgtcgcgt atccttccgt
     4561 cgactggaaa aaattcggcg acgatttcat cgcgtcgctc gggctgaaac gttcgcaccc
     4621 gacgacgcag atcgaacatt acgacaacct cgctgcgttc ttcgacgggc tgaagcgcat
     4681 caacaccatc ctcatcgatc tttcgcgcga tttctggcaa tacatttcca tgaattattt
     4741 ccgccagaaa gtgaaagagg gagaagtcgg ttcctccgcg atgccgcaca aagtgaatcc
     4801 catcgatttc gaaaatgcag aaggcaatct cggtttcgcc aatgcgatct tcgaacacct
     4861 gagcgcgaag ctgcctgttt cccgcctgca gcgcgacctc accgattcca ccgtgctgcg
     4921 caacgcgggc gtcccgttcg cgcatacgat cattgcgctc aagtcgctgc agaaaggaat
     4981 ttcaaaactg ctgctgaatg aagatgcgct gaacaaagac ctcgagcaga actgggccgt
     5041 tgttgcggaa gccatccaga cgatcctgcg gcgcgaaggt tatcccaaac cctacgaagc
     5101 gctgaaagac cttacccgcg gaaatgcatc cgtgacaaaa gattcgatgc atgcattcat
     5161 cgaccggctt tctgtttcgg atgagatcaa aaaggagctg aagaaaatca cgccgcagaa
     5221 ttatacgggg atctgagatc cgaaaactga aaaaacgaaa gattgaaaag atacggggcc
     5281 atggaacatt cgccgggaac tatgcgttta cgatcttttt aattcctgtt ttaagtttat
     5341 cgttgatcag ctgatccggg cagaaacatc aatgccggct gcagaatttc atcacctgcc
     5401 tgaaccttcc cgggcgaaaa ggctcgctta aagatccctg aacatggacg gtaaaaattc
     5461 caacggttca tgtttccttt tttaattttt tgaatgtcct gttcttttga atgtttccgg
     5521 tcttttcttt ttaatttccc gccctgtcta tgttcgtctc gggtttcacc atcgtccgta
     5581 atgcagtaaa attcgattac ccggttgcgg aagcgatacg gtccatcctt cctttgtgcg
     5641 atgaagtagt tgttgccgtc ggaaaatccg aagatgaaac gctgcagctt atccggtcga
     5701 tcggcgatcc gaaaatccgt attgttgaaa cggtgtggaa cgatgcgctg cgcgaaggcg
     5761 gacgtgtact ggcggatgaa acgaacaagg cgctcgatgc tgtttctgaa aaagccgact
     5821 ggtgctttta tatccagggc gatgaagtga tccatgaaaa atatcatccc gcgatacgcg
     5881 aagcgatgct gaaatggaaa gacgacaaaa acgtggaagg cctgcttttc gattaccggc
     5941 atttttacgg ttcatacgat tacgtgggcg attcgcggaa atggtaccgt cacgaaattc
     6001 gtgttgtgcg caacgacagg aatatccgtt cgtaccgcga tgcgcagggc ttccggaaaa
     6061 aggacaacag caagctgcgc gtgaaaaaat cgggtgcgga agtctttcat tacggatggg
     6121 tgaaaccgcc gcaggcgcag caggcgaagc aggaatcgtt tcacaaaatg tggcacgacg
     6181 acgaatggat gaagaaaaat attcccgtcg cggaggagtt cgattattcg cagatcgatt
     6241 cgctggcgaa attcagcgga acacacccgg ccgtgatgaa agagcggacc gaacgcatga
     6301 actggaaatt ttcattcgac ccgacgaaga aaaaatgggg attcaaaatg cgcctgctga
     6361 tgtggaccga aaaaacgacc ggctggcgtc ccggcgaata caaaaattac atcctgctga
     6421 agtagctcac ggcatcccgt tcacttcccg gaaatacgtg atcatgtctg ttgaaggaag
     6481 cttttccgtc cacccgcttt cacggatcat cgtcaccagc tgcccgcggt ggaacgttcc
     6541 gtggttcatg cagtgaaaga tcatctgcgc attttgcatc gtgtacgggt ttcctttcat
     6601 gtctttgaac gaagtcgacg cggtgaaaaa actttcgtcc tgcatttcga gaaacgcgag
     6661 aaaatcctcc gctcctttca cgaacatccg cggatccgca tccgccggga gcgacctgct
     6721 gttccagtcc gccggcgaaa cgcctttcag ccgctgaagc cagatgtgct ccgcgtccca
     6781 gatgtgaaat gccgttttgc gaatagtggg gaaactgctt ttcgtttcgc gcaagaacga
     6841 ttcttcactc atccgcgaaa aaacatccgc gatccttttg tccgcccaga gattataacg
     6901 ggcctgcgcc agcaggaatt ctttcgcatc cttcatcatc agctattcat tgccagtcat
     6961 tcatcgtgct tattcccatc cctgcaccag cacatcgacc aggtgcatca ccttgatgtt
     7021 cttcttttgt tttttgatga agccgtcgag gtgaaggagg caggaaagat ccgtcgagat
     7081 gatgtattcc gcaccggcgt cttccgccgt tttcaatttc tgctccgcca tgcccacggc
     7141 gatgttctcg tatttaacgg aaaacatccc gccgaatccg cagcacacat cgctcttttc
     7201 catctcgcgc acttcgagtc cgcgcacttt cgaaagcagc acgcgcggct cccggtgaat
     7261 gccgtattcg cggaggccgc tgcaggaatc gtgaaaaaca gcaacgcctt ccatcttcgc
     7321 gccgagatct gtgatcttta aaatattcac gagaaaatcc gagaactcgt acatgttctt
     7381 ctgcacctgc cggaactggt tgtgcaatgc cgtgttatgg aacatttcgc cgtaataatt
     7441 cttcacgaag ccgggacagg aagacgaagg agaaacaata taacggtcgc tctggaattc
     7501 cctgatgaac ttctcgccca cttccttgca gtggtcccag taaccagcat tgaacgcggg
     7561 ctggccgcag cacgtctgct cgtggttgta attcacgccg catcccacac gctccagcac
     7621 tttgatcatg ttcagcgcgg tatcaggata aagctgatcg acgaagcagg gtatgaataa
     7681 atctactatc atttccggtt ttccgttttc agccgaccgt tttcagtttg atctgtcata
     7741 aatcgcacga cataaaactg aaaaccgccg gccggactat ttttatctga cattctgaga
     7801 tttcggtacg cgcagcagca gtaaaaaccc gagcacaaaa ataacgatga gcgacaaaac
     7861 cgactggcgt atgccgcccg ttattcccgc gatcagcccg aacgtcatgg ttccgatggc
     7921 gagcccgatc ttttcgctta cgtcgaaaaa gctgaaatac gaagcatggt cttccgtttc
     7981 cgggagcatt ttggaatacg tcgaacgcga catcgactga atgccgccca tgacaagtcc
     8041 gacgctcgcc gcaacaatgt aaaactgcac cggcttgtac acgaactgga acgtgccgta
     8101 gcagatgagt atccagagga agatcacgat accgatcgtt ttcaggttgc cgatcacgcg
     8161 ggaaagacgg gacatgagga atgcaccggc catcccgaga aattgtatga gcaggatgga
     8221 gatgatgagg ctctggcttt cgtgtccttc ggcaagcgtt tcggcgcgct ggtatacatc
     8281 ggcgaggtaa tccgcatagc ccggtgccag gcggacatca tccgccgcaa ccgtcccgcg
     8341 catatcctgc tgcacggttt cgatattttt ccggatggcg gctgtgcggg atcccgcgga
     8401 aagcgtgtcg gaaagtttcc ggttcagctt catgacagcg tcgatctgcg accgcacctg
     8461 ttccggcgtg atgctttccg ctccgttcac catcgttatt ttccctgctt tcgcatctgc
     8521 ctgcacctgc acgatcgccg actgcacgtc acccggcgat tgccagtcga ccgcctccgt
     8581 agcaaaaagc gtcgccatga tcatcacggt ctgcacgccc atgttgtaca tgaaatacga
     8641 accgagaaaa cggcgcagct gcttgcgggt gaggatgtcc ctgaacacga gccgcagctc
     8701 gcggtagcct ttcgtcacga tgttctcatt cgttttattc tcgtgcgctt ttcctttcgg
     8761 caggcgccgg aatgtgatct gcgcaaaacc gatccaccac aaaccgacga gaaaaaacga
     8821 ataccggatg tgcgggatca catgcgtgaa aacagccagc acgaggataa gaataagcag
     8881 cagggagctg ccgaaataac ccatgccgaa cccgcgtgcg cttactttgt cctggtcttc
     8941 cggcggtgcg atctcgggca ggtaagcgtt gtaaaaaaca aggcttcccc agtaacccac
     9001 gctcgcgaac atgagcgcca gcatgctgag aagaatgtgc tgtgtgctga aaaaaccgag
     9061 cagcatgcag cagcccgcac cgagaaagca gaagaaccgg aggaatttct tcttgctccc
     9121 ggaaaaatcc gcgatgccgg aaagcaccgg ggaagaaagc gcgacgatga tataggaaat
     9181 agccgcgata taggagtaaa attctgtgct gttgaacgaa gcgccgccga tcgcgatacg
     9241 gccgccgaga tctttcttaa caacggcttc gtaatagatc gggaaaatgg cggaagaaat
     9301 gacaagcggg taaaccgaat tcgcccagtc gtaaaatgtc catgcgcgaa ttactttggg
     9361 gtcgcctttt ttgagcataa cgccaaatgt aggcagcggg tcctgcttct ccggtgtttg
     9421 ttgaaaagta ccggtggcgg ttgaaaagtc cgcggtccgg gttccccgcc cgaaactgaa
     9481 aaccgttaac ggaaagcttc aatccgtttc cccgaaaaac tttttattga accccacgag
     9541 aaaaactttt tccgttgtgc gcgtgatcgc cgtataaagc catcgcagga attccgtatt
     9601 gatcatttct tccgtcaggt aaccctgctc gacgaaaacc gctttccact gaccgccctg
     9661 cgccttgtga cacgtgacgg cgtaagcgaa cttcacctgc agcgcattga accagggatc
     9721 ctctttcatt tttttgtaac gcgcgccttt cgaaagctcc gcgtaatccg ccgcgacatt
     9781 ttcatacagc tgctgctgcc gctgcggggg caatgccggc gactcggtat tcagcgtatc
//
LOCUS       DASTSF010000093        19085 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1280947,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000093 DASTSF010000000
VERSION     DASTSF010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 19085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19085
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1280947"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(483..803)
                     /locus_tag="VFU15_02810"
     CDS             complement(483..803)
                     /locus_tag="VFU15_02810"
                     /inference="COORDINATES: protein motif:HMM:NF020397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEU4716730.1"
                     /translation="MKKNYTTVPGYIALQPQEIRPKLKKLREVILSVAPGADEMISYG
                     MPGFRQDGILAWFAAFKNHYSFFSRPGALDPFRDELRIWSGSKNRSSCGNCFESLCTF
                     SDNS"
     gene            complement(840..2864)
                     /locus_tag="VFU15_02815"
     CDS             complement(840..2864)
                     /locus_tag="VFU15_02815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716731.1"
                     /translation="MQTNISTWTVSRLCIAAILFVTAWCARVTYTFSDPQLSLLTSQA
                     LLENGSINLYRYKTAMPSDQFSSGNWKFTTCGRKETCYYSYPLGNSFLSLPFVAAANF
                     FGLDMANTEQDAFLQHLLATCCTIVLFFLLCRIARLFLDEIPALVAATAISFGTGFIS
                     VVTTALWSFDIELILVLVTLDYLLRYELKLAQKKRPVLLGLLVFFAWFCRPSAAIVAA
                     LAGAWMFFRQRRQLPLFILSLLVPLGVFMWLSYRSYGVLFPSYYNPLYWEHTASSGSG
                     FFQRMSALLFSPARGLFLFTPLLLLSFGGFFSAALRKNKIYIAMTGWFLLQLVMTATQ
                     KNWWGGWCFGPRLMTETLPSLAIAGFLVFGEHLQKGRARQAMLLIAPLAAFGIFTHTV
                     QGLYNKETVDWNDFPNIDGRPDYYIWNWKQTQLFADAAGNHVKRKEAEILQELSSVLP
                     ELRPGANLLYGEPGAITRTIYDRWEKNGTLPVHVHNNLYSIEQSAKDTFWITASDFEM
                     IRGMECYRILAPEGQPSLGDYLRSMENYNVLLSVSDEGSYSLSESTRAYFRSKGSAID
                     SLHFREGYAALFLHGKLFAEQFEKNDTAKVSWTAKTFSLRATSNGKTTGNNAQLLLNG
                     QNLSGNHRGFNVLVVDDTGNVIDNTCFDTHVFDARVPKVFAVVRSDKHRQ"
     gene            3106..4647
                     /locus_tag="VFU15_02820"
     CDS             3106..4647
                     /locus_tag="VFU15_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020602070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HEU4716732.1"
                     /translation="MSLIKTHGCAVFGINATTVIIEVNIDTGINFHIVGLADSAVKES
                     QQRIDAALKNNAFRVPGKKITVNLAPADIRKEGSAYDLPIAVGILCASEQLNAERVSE
                     FVIMGELALDGILRPVRGALPMAIHAKECGFSGIILPEENAKEAAVVAGLTVYGVNHL
                     RQVTDFFEGKSGLVPATIDIEKEFAGAVNFSDADFSDVKGQENIKRALEIAAAGGHNI
                     LMVGPPGAGKTMLAKRLPGILPPMTLKESLETTKIHSVAGRTAKPGGLVTARPFRAPH
                     HTISDVALVGGGGIPQPGEISLAHNGVLFLDELPEYKRTVLEVMRQPLEDRVVTISRA
                     KFSVTYPAGFMLVTSMNPCPCGYYNHPEKECVCPPGTVQKYLNRLSGPLLDRIDIHIE
                     VTPVGFGQLTDRRPAEPSASVRERVVRARTVQLQRFGAHENIYCNAQMNANLLREVCF
                     IDDTGRQLLKTAMERLRLSARAYDRILKVSRTIADLENSDDIRSEHLAEAIQYRSLDR
                     ENWAG"
     gene            4987..5364
                     /locus_tag="VFU15_02825"
     CDS             4987..5364
                     /locus_tag="VFU15_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013455530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU4716733.1"
                     /translation="MDSISPNIFVADIKQTIDFYKQLGFSIVATVPEQDDIVWAMMTC
                     GNVTFMFQTFESLGNDLPVISRQNGGSLLLYIQTTQIRKFFDQVKDTVKVVKELEKTF
                     YGATEFSIEDNNGYLLTFAEDEK"
     gene            5354..5854
                     /locus_tag="VFU15_02830"
     CDS             5354..5854
                     /locus_tag="VFU15_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEU4716734.1"
                     /translation="MKSKTNIKLPLTDIEKASLRKNKIKISDIPDFATDELEVLLNAT
                     TERVREIYALAEFQTVPSIGIKFAEDLVFLGYYSLNELKNKDGAKLVDEYEQKKGYWI
                     DPCVEDQFRLVVNFANTNDTKKAWWDFTEERKKFRTENGYPPNRPQKPWYETLEYERK
                     DKKAKR"
     gene            6096..6752
                     /locus_tag="VFU15_02835"
     CDS             6096..6752
                     /locus_tag="VFU15_02835"
                     /inference="COORDINATES: protein motif:HMM:NF019126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEU4716735.1"
                     /translation="MAYSNDTPIGTIIPFAGYFYLPNTTFEQMKVLQPELSERDYNRG
                     LQNATELAESGWMLCDGKPYRYTTNPADILFQLCRVLSQAWGGNEFEIGASFRVPDLR
                     GVFLRGVTTFSSLDPDASSRTKIYPTDNFGEKGNQVGSFQGDDFKKHDHKFQAPITGE
                     KVGYASDSSVRANFKESATLVNGGEETRPKNAYVHYLIKAAHQSLDLPQIFENNSIVE
                     "
     gene            complement(6858..8342)
                     /locus_tag="VFU15_02840"
     CDS             complement(6858..8342)
                     /locus_tag="VFU15_02840"
                     /inference="COORDINATES: protein motif:HMM:NF025067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4173 domain-containing protein"
                     /protein_id="HEU4716736.1"
                     /translation="MNRFIVLFPPLLFLSDLLFWHTTPGINVLLFSVVMIAVLLRRYP
                     SALRRRPVQVTAGGALLSALMIFLHASHWALFSCILSLLMFSMFASEAGLVSFFHASL
                     QALVSIFDFPLTFAHHREQFSKNNRYRGKTRFYALVVLLPLLTATGFYLIYLWGSPHF
                     FALNASFLNVIQGFFRNFSFAHFFFIAACGVALIILLLRGYNRIRTDKGLPFDLRRKR
                     LPYFGRKTALKEEALAGLVTLVLLNALLLVVNVTDVEKVWIHFVLPEHFSLKDFVHNG
                     TWILSISILLSMIVTLRFFRGNLHFYSRNRWLKRLALAWLFQNAVLTFSVFLRNYHYM
                     TWHGLAYGRIFVFFFLALVMTGLASIAWKIVRTKSSYFLLRLNSAVIYGAILFAALFN
                     WDKVIVNYNLHAFNPAQIDVDFYLEAHEDVLPIVYNNLDVIRTQIEAHQKNPVHWISY
                     HNIDEFTDHLEARRNSFLLKAEHGPWQSFTFADAEARKKMAEMK"
     gene            8545..10152
                     /locus_tag="VFU15_02845"
     CDS             8545..10152
                     /locus_tag="VFU15_02845"
                     /inference="COORDINATES: protein motif:HMM:NF018449.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HEU4716737.1"
                     /translation="MKKMYLALTLQLFSAALFSQQYWQWGHEAGGAGNDEAMAIATDP
                     NGYSYVTGYFNSTASFGPNSLTSSGGADIFVAKYSPGGTCLWVRGFGGVNNDAGHGIA
                     LDAAGNCYVTGDTGMCAVVRKYNSAGNVIWTTVQANAASANCSSRAITTDSAGNSWIT
                     GYSGGGAQLFGSHVLNGPGGYVVKFDTAGVPVFATKLATAGAIDLYGVTHDAAGNCYA
                     TGYLQGQDNIGSLQFTSTGSRDAVLIKVDTSGNFEWLKQSLALSGGTSVMPNGICADK
                     GDGIYFCGDLAGKTVFGNDTLPGDSAGGSNIFIVKYDTAGTALWAEEPTLTAQGPGQL
                     HAYAVATDTSGNAYMTGVFSSDLVFGSTPLLDGILGMNTFIVKFEPNAGTCQFTIQST
                     GSNPGAFGLGISTDSYGGVYIAGFDKAPVVFGSNTTNFIGGEDIFVARIAEGKESGIN
                     ETDFSAQVSFFSSGDAFQLSVADEKMLYVHPTLNIYDMTGRLVKTIAVNELQTTISSA
                     DLAPGIYSWELTGSQQRFASGKTALCR"
     gene            complement(10327..11559)
                     /locus_tag="VFU15_02850"
     CDS             complement(10327..11559)
                     /locus_tag="VFU15_02850"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011166031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate dehydrogenase (NADP(+))"
                     /protein_id="HEU4716738.1"
                     /translation="MAKIKVANPVVELDGDEMTRIIWKFIKEKLILPYLDIDIKYYDL
                     GIEYRDQTNDQVTIDAAEAIRKYHVGIKCATITPDEARVKEFHLKQMWKSPNGTIRNI
                     LDGTVFREPIVCKNVPRLVPNWTKPIIVGRHAFGDQYRATDAVFKGKGKLTMTFTPEG
                     GGEAQTFEVYNFKGDGVALTMYNTDESIRGFAHACLNMGLMKKWPVYLSTKNTILKKY
                     DGRFKDIFQEVYEKEYADKFKAAGIVYEHRLIDDMVASALKWHGEFIWACKNYDGDVQ
                     SDSVAQGFGSLGLMTSVLVTPDGKIMEAEAAHGTVTRHFRDHQAGKPTSTNPIASIFA
                     WTRGLDFRGKLDNNAELQKFAKALEEVCVETVESGKMTKDLAVCIHGNNVKLGEHYLN
                     TEDFLSAIDTNLRKKLAL"
     gene            11717..12136
                     /locus_tag="VFU15_02855"
     CDS             11717..12136
                     /locus_tag="VFU15_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019532630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEU4716739.1"
                     /translation="MSSPVSITVRTVISAPVSRVWECFTQPQHITRWNFAVPEWHCPA
                     AENDLRPGGKFSWRMEAKDGSFGFDFAGTYTDVRPNELIAYTIGDGRKVHVTFMTDGD
                     STEVTETFEAESQNPVEMQRGGWQAILDNFRKHAEAG"
     gene            complement(12144..12698)
                     /locus_tag="VFU15_02860"
     CDS             complement(12144..12698)
                     /locus_tag="VFU15_02860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716740.1"
                     /translation="MPVKRDFDFERIIRDTCWKNKIGAIAGIVVLFLLSIFFIYFGIK
                     IPFAPILILGLLPLGGIALVVYRFRGDLKGEDYWIDELVHHPERIAWIKPVRVQHTAY
                     YVVTLYHESYYQLLDINGLAIMIKCNSPEDQLLFLRGVKRYTPHAHIGFSHVVQSLYN
                     RNPAEFIRMVTESRAYLPVGTMNV"
     gene            13132..14199
                     /locus_tag="VFU15_02865"
     CDS             13132..14199
                     /locus_tag="VFU15_02865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716741.1"
                     /translation="MRKIRFLFIFPLLLFPFSFLHSQHAVITVWKDSAMGAIYNKATQ
                     TVAYGKPDKKGTYKIYLCDTLGNNEHMLTYSGWSPDRHQWAEEWDPSGKFLYCYVEKT
                     EYVKEKGHKRKPVDACPGYGAYTDLWAITRDGKKAWKLVDEPNSYNSGIIHSAISPDG
                     KMFAWSERIKAPQFNMNLAAGSYVIRVADITVDSCPHFSNIRTFQPGGIDATNELDGI
                     CPGDSVITFYSTFESKNLFATPVYKINILTGKITKLTTASFAQAGTFTPDGKHIVYMT
                     GDSCNIFPPQPQGADWWIMNTDGSGKRRLTYMNKKGDAQCDNHYRLAGTVSFMSDNCF
                     FGGVMTKSLGLVGYTVKVRFE"
     gene            14317..14769
                     /locus_tag="VFU15_02870"
     CDS             14317..14769
                     /locus_tag="VFU15_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009579928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEU4716742.1"
                     /translation="MEKLIVTNTVAISATPQRVWDVLTKPEETKKYMFGCETVSDWKE
                     GSALLWKGEYEGKEMVFVSGFILEIVPGKLLRYSVIDPNAAYPQTPENHLRVTYEITP
                     GNGSVTLTVTQDGFEGAAEGEKRYRDTYNNGDGWNPVLQAIRKVAEER"
     gene            14825..15805
                     /locus_tag="VFU15_02875"
     CDS             14825..15805
                     /locus_tag="VFU15_02875"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014796106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex E1 component
                     subunit beta"
                     /protein_id="HEU4716743.1"
                     /translation="MRTIAFREALREALSEEMRRDEKVFLIGEEVAEYNGAYKVSQGM
                     LAEFGEKRVIDTPIAELGFAGIAVGAAMNGLRPVVEFMTFNFSLVAIDQVINSASKML
                     SMSGGQFNIPMVFRGPTGNAGQLAAQHSQAFESWYANCPGLKVVVPANPYDAKGLLKS
                     AIRDNDPVIFMESELMYGDKGEVPESEYLLPIGVADIKREGTDVTIVSFGKILKVALA
                     AAAELEKENISAEVIDLRSVRPIDYATVIRSVKKTNRLVIVEEAWPLANISTDLTYHV
                     QHHAFDYLDAPVRRVVSADVPLPYAPTLIEVALPGIAQTVKAVKDVMYVS"
     gene            16214..16561
                     /locus_tag="VFU15_02880"
     CDS             16214..16561
                     /locus_tag="VFU15_02880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716744.1"
                     /translation="MRVPSCILIFAFVFSLAKLSAQSFCGYEHSNYAGIVGASYNPAS
                     LADNNYSLDILLFGFDVEGANNYVGVKRKDMFHPDFGPEHFWLRDRPFKKSVFFRNEI
                     LLPGIMFSNEKYG"
     gene            16571..17665
                     /locus_tag="VFU15_02885"
     CDS             16571..17665
                     /locus_tag="VFU15_02885"
                     /inference="COORDINATES: protein motif:HMM:NF040164.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5723 family protein"
                     /protein_id="HEU4716745.1"
                     /translation="MKVRTYFNVDGIQPPLAHLLAEGFTDPSDYNTGYHNRHIGINEC
                     TWFEIGGTYAKTIWTGADRFVSIGVRPKFLLGLAAAYVFIDNADYEFYNDSVMALFGG
                     NLKFGHSDNFSFNENFQPSYRIGFNPGIGLDAGIIYEFRPGDMQKNEKPGKEWPGFRD
                     RPVYKYRIGASLTDLGIIHFRSGAFTDQYSVSSDAWNVAHDVFNATAPAPLFNNFNLQ
                     YHGAGEGQSFWMRTPLALGLFYDYRFRENLFFSAQSFTGLYLRGTDFKKVHELTRISL
                     TARYERRWYGAWVPVSFTRMGTFSMGAGLRLGPVIIGTTDILNLTMKNKRIYNADIYF
                     ALKVPLFPLGSGRKKKTKVRKTTDVDKCPG"
     gene            complement(17628..18602)
                     /locus_tag="VFU15_02890"
     CDS             complement(17628..18602)
                     /locus_tag="VFU15_02890"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEU4716746.1"
                     /translation="MDFYSAPRGWFPWKPAADGNFHWIYLGNKKFDEPFFEETIHRRL
                     SLEENSSFRENRSSAASLVSASQNVNAAEPAAFIFHVSRCGSTLLSQLLSEDPANIVL
                     PEVPLFDDLLLRMHEKKNFGDISAEQLLTASIKLMCAKKNPEENSAFIKLDSWHIFHY
                     AVLRKLYPRTPFVFLYRHPAEVIRSHARQAGMQMIPGLLPSSLTGIELTEEMLTNHRF
                     YAGKMLERFFECFLRAKETDGNFFSADYADGIENAAFHLLRQCGAEPAAGLKERFTSR
                     LLFHSKRPGEKFAAESSETGIDGFSERAAGLYEKVRATRDIYQRPLFS"
     gene            complement(18614..>19085)
                     /locus_tag="VFU15_02895"
     CDS             complement(18614..>19085)
                     /locus_tag="VFU15_02895"
                     /inference="COORDINATES: protein motif:HMM:NF016974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aspartyl/asparaginyl beta-hydroxylase
                     domain-containing protein"
                     /protein_id="HEU4716747.1"
                     /translation="YFSAVVDSFRCEKEAVRLLRLAPGSEIRTHRDIRLAYPFGNFRV
                     HVPVVTGPQIDFIVGGERIEMKEGECWYANFDLPHSVKNHGATERVHLVIDCVRNEWS
                     DELFANAGYDFEAEKKALAPDDETKRRMIEELSRIDTPASRDILEKLLSGLSEK"
BASE COUNT         4714 a         4810 c         5100 g         4461 t
ORIGIN      
        1 agtttctcag gttttcgtcg tacccgtatt gacgatagtt tttcaccaca tcaatgagct
       61 tgtctgtgtc aagtttttta agccgttctt ctttgccggt catttgcagt agttctgaaa
      121 ttacgtttat ggtttgcgaa aggataatga ctgcagcgat aatgttggga aacaaaacga
      181 cggccgttga acatcaatca tcctttcaaa gctattgcat tcccgctttt taccaaaacg
      241 cctgtcgatt acccggcagc aatcgtttgt aaacgacaac gcccgattat atccgcttaa
      301 ctccgtgtgc gctgatgcgg gaatttgggt ggaagcgaaa ggatttttta tttcgtgccc
      361 ttgatgttta ataattgcgc gggtgagtaa aaggttgttc cgtggcgatc ttatttgtga
      421 aaagttaaag agacgaaata tatgaaagcg ggttacaagg aattgcggct gtaccagctc
      481 gcctatgagt tatcactgaa agtacacagg ctctcgaagc agtttccgca ggacgaacgg
      541 tttttactcc cggaccagat ccggagctcg tcgcggaaag gatcgagcgc tcccggccgt
      601 gagaaaaacg aataatggtt tttgaacgcc gcgaaccagg cgagaattcc gtcctgccgg
      661 aatcccggca tgccgtagct gatcatttca tctgcgccgg gcgcaacgct gagaattact
      721 tcgcggagct ttttcagctt cggccggatc tcctgcggct ggagggcgat gtatcccggg
      781 acggtggtgt agtttttttt cattggcgtt gctgattttc atttagccgg tgcagcccgc
      841 tattgccggt gcttgtcgct ccgcaccact gcgaaaactt tcggcacccg cgcatcgaaa
      901 acgtgcgtgt caaagcaggt attgtcgatc acatttccgg tgtcgtcaac cacgagcacg
      961 ttgaagccgc gatgattccc cgaaaggttc tgcccgttga ggagcaattg cgcattattg
     1021 cccgtcgtct tgccgttcga agtcgcgcga agcgaaaacg ttttcgccgt ccacgaaact
     1081 tttgccgtgt cgtttttttc aaactgctcg gcaaagagct tcccgtgcag aaaaagcgcg
     1141 gcataacctt cgcggaaatg caggctgtcg atcgcagaac ccttgctgcg gaaataggcg
     1201 cgcgtgcttt cggaaagcga ataagatcct tcgtcgctca cgctaaggag cacattgtaa
     1261 ttttccatgc tgcggagata atcgccaagc gaaggctgcc cttccggtgc aaggatgcgg
     1321 tagcattcca ttccgcgtat catttcaaaa tccgatgccg tgatccagaa cgtatccttt
     1381 gcgctctgtt cgatgctgta aagattattg tgaacgtgca cgggcagcgt tccgtttttc
     1441 tcccaccggt cgtaaatagt gcgggtaatg gcgccgggtt cgccgtacag cagattcgca
     1501 ccgggacgca gctccggtaa aacggaagac agttcctgca gtatctccgc ttccttccgc
     1561 ttgacgtgat tgcctgctgc gtcggcgaaa agctgcgttt gtttccagtt ccagatataa
     1621 taatccggcc tgccgtcgat gttcgggaaa tcattccagt ccacggtttc cttgttgtac
     1681 aaaccttgta cggtatgcgt gaagatcccg aacgcagcga gcggtgctat cagcagcatc
     1741 gcctgcctcg ccctgccttt ctgaagatgt tccccgaata cgagaaatcc ggcgatcgcc
     1801 agcgaaggaa gcgtttcagt catcaggcgc ggaccgaaac accagccgcc ccaccagttt
     1861 ttttgcgtcg cggtcatcac cagctgcagg aggaaccagc cggtcatcgc gatataaatt
     1921 ttgtttttcc gtagtgcagc ggaaaaaaat ccgccgaacg aaagcagcag caagggtgtg
     1981 aagagaaaca atccccgcgc cgggctgaac agcagcgcgg acatgcgctg gaaaaaaccg
     2041 cttcctgaag aagctgtgtg ttcccagtaa agcgggttgt agtatgaggg aaatagcacc
     2101 ccgtaggaac gatacgacag ccacataaaa acaccaagcg gaacaagcag cgagaggatg
     2161 aacaggggaa gctgcctgcg ctgccggaaa aacatccatg cgcccgcgag tgcggcaacg
     2221 atcgccgccg acggccggca gaaccatgcg aagaacacca ggagtcccag taacaccggc
     2281 cgttttttct gcgccagttt taattcgtac cgcagcaggt agtcgagggt caccagcaca
     2341 aggatcagtt caatatcgaa actccacaac gcggtcgtca cgacactgat gaatcctgtt
     2401 ccgaaggaaa tcgcggttgc cgccaccagc gcaggaattt catcgaggaa cagccgggcg
     2461 atcctgcaga gcaggaaaaa gagtacaata gtgcagcagg tcgcgagaag atgctgcagg
     2521 aaagcgtcct gttccgtatt cgccatgtcg agcccgaaga aattcgccgc cgcaacgaac
     2581 ggcaggctga ggaatgaatt gcccaacggg taggaatagt agcacgtttc ctttcgtccg
     2641 cacgtggtga atttccagtt gccgctggag aactgatcgg agggcatagc ggtcttgtaa
     2701 cgataaaggt tgatgctgcc gttttcaagc agcgcctgcg acgtcagcag tgagagctgg
     2761 ggatcgctga acgtgtaagt gacgcgcgcg caccatgccg tcacaaacag gatcgcagcg
     2821 atgcagagac gggaaacagt ccaggtggag atattagtct gcatgaaaag tcgttcgttc
     2881 gccgaaggta gaggcttcac cgggtttgcg caaaaaggca ggctgcgccg ggcacaagtc
     2941 agtggtaatt gcttataccc ggttgttgtc ggataaacgc gggaagtgat ttggtttttc
     3001 agcagaaaaa aaatttcttt gccgccgttt aaaaatttcc tgcgggagga agatcgttat
     3061 tgctgttttt gaatttgttt ttgtttttaa agaaagaagg atcgcatgtc actcatcaaa
     3121 acccacggct gtgccgtatt cggcatcaac gccaccaccg ttatcatcga ggtcaatatc
     3181 gacaccggca tcaatttcca tatcgtcgga ctcgcggaca gcgcggtgaa ggaaagccag
     3241 cagcggatcg atgcggcgct gaagaataac gcgttccgcg tgccgggaaa aaagatcacc
     3301 gtcaaccttg caccggccga catacgcaag gaaggttccg catacgacct gccgatcgcg
     3361 gtcgggattt tgtgcgcatc ggaacagctg aatgcagagc gcgtaagcga attcgtgatc
     3421 atgggcgagc tcgcgctcga cggcatcctg cgtcctgtgc gcggcgcgct gcccatggcg
     3481 atacacgcga aggaatgcgg cttcagcgga attatcctgc cggaagagaa cgcgaaggaa
     3541 gcagcggtcg ttgccgggct cactgtttac ggcgtgaacc atttgcgcca ggtgacggat
     3601 ttcttcgaag ggaaaagcgg gctggtccct gcaaccatcg acatcgaaaa ggaattcgcg
     3661 ggcgccgtga atttttccga tgcggatttc tccgacgtga aaggccagga aaacatcaag
     3721 cgcgcgctgg agatcgcggc ggcgggcggt cataacattc tcatggtggg tccgccgggc
     3781 gcgggaaaga ccatgctcgc aaaacgtctc ccgggcattc ttccgcccat gacgctgaaa
     3841 gaatcgctgg agacgacgaa gatccattcc gtggccgggc gaacggcgaa gcccggcgga
     3901 cttgtcactg cacggccttt ccgtgcgccg caccatacca tcagcgacgt cgcgctcgtg
     3961 ggcggcggtg gaatcccgca gccgggagaa atttccctcg cgcacaacgg cgtgcttttc
     4021 ctcgacgagc tgccggaata caaacgcacc gtgcttgaag tgatgcgcca gccgctcgag
     4081 gaccgcgtgg tgacgatctc ccgtgcgaag ttttctgtca cgtaccctgc aggtttcatg
     4141 ctcgtcacgt cgatgaaccc gtgcccgtgc gggtattaca atcacccgga aaaggaatgc
     4201 gtgtgcccgc cgggaacggt gcagaaatac ctgaacaggc tgtcgggtcc gctgctcgac
     4261 cgcatcgaca ttcacatcga agtgacgccg gtcggtttcg gccagctgac ggatcgccgt
     4321 cccgcggaac cgagcgcgtc cgtgcgtgaa cgcgtcgtgc gtgcgcgtac tgtccagctg
     4381 cagcgtttcg gcgcgcatga aaacatttac tgcaatgcgc agatgaacgc gaacctgctg
     4441 cgcgaggtct gcttcatcga cgacacggga aggcagctgc tgaagacagc gatggaacgt
     4501 ctgcgtcttt ccgcgcgtgc gtacgatcgt attctcaaag tttcgcgaac gatcgcagac
     4561 ctggaaaatt ccgacgacat ccgttcggaa cacctcgccg aagcgatcca gtaccgcagc
     4621 ctcgaccgcg agaactgggc gggatgagca gaaggaggtc gcggggaaaa acaagcgtag
     4681 cccaagggga aagatccggt tgtatttgga tttattttaa atgaattaga tttgatgaac
     4741 aagcgccagt taagcagcag gagtgaagtg agagtggttt ttggctgcaa tactggaagc
     4801 aattcagtcg actgttcgtt cgacaaagcc aaggaatttg aaacgagcga aatagtaaac
     4861 ggcgtataag cgcaatgcct tacgaaatga taagtaaaaa tcaaaaataa cagtcactac
     4921 tgcgcacggg cgcaaaccat ttattgcaac cctgacgacg caacccgagc agacaaacaa
     4981 aaaaatatgg actcgatatc accgaacatt tttgtagcag atattaaaca aacgattgat
     5041 ttctacaagc aattaggatt tagtattgtt gcgacagttc ctgaacagga cgatattgtt
     5101 tgggcaatga tgacttgcgg aaacgtgact tttatgttcc agacctttga aagtttgggt
     5161 aacgatttac cggttatttc aaggcaaaac ggaggttctt tgcttttgta tattcaaaca
     5221 actcaaatca ggaaattctt tgaccaggtt aaagacacgg taaaagtggt taaggaactt
     5281 gaaaaaacat tttacggcgc cacagaattt tctattgaag acaataatgg ttatttgttg
     5341 acttttgccg aagatgaaaa gtaagacaaa cattaagctg ccgctcacag atattgaaaa
     5401 agcgagcttg agaaagaaca aaatcaaaat ttcggacatt cctgacttcg caacggatga
     5461 attggaagta ttgcttaacg caaccactga aagggtcaga gaaatttatg cgctggcgga
     5521 atttcaaacc gttccgtcga tcgggataaa gtttgccgaa gacctcgtgt ttttagggta
     5581 ctattcactc aacgaattaa aaaacaagga cggggcaaaa ctcgtagacg aatatgagca
     5641 aaaaaaaggc tattggatcg acccttgcgt tgaagatcag ttcaggttgg tcgtaaactt
     5701 tgcaaacaca aacgacacaa aaaaagcatg gtgggacttt acagaagaac gaaagaagtt
     5761 tcgcacggaa aacggttacc cgccaaacag acctcaaaaa ccgtggtatg aaacattaga
     5821 atatgagcga aaagacaaaa aagctaagcg ctgacgcgtg ttttgcatta acaggcaggc
     5881 tgtgcgaata gaaatagttt aataaacttc ggcgggacag acagtttacg gtgtcaacca
     5941 gccagccagc cggaggcgcc cgtcaacaca aaatcgtaaa ccattgactt taatttaaag
     6001 acaactactg aaacacaaac aataaattgt tgatcaaaat atcttaacgg ggtagccgaa
     6061 aaccgaatag agtaggtgaa ttataaaatg taaatatggc atacagcaac gatacaccga
     6121 tcggcaccat tatacctttt gccggctact tctacttacc aaatacaact ttcgagcaaa
     6181 tgaaagttct tcagcctgag ttaagtgaga gagactacaa tagaggactt caaaacgcaa
     6241 ctgaattggc agaatcagga tggatgttgt gcgatggaaa accgtacagg tacacaacta
     6301 atccggcaga tattctattt cagttatgca gagttctttc acaagcctgg ggaggaaatg
     6361 aatttgaaat aggggcatca tttcgagtgc ctgacttaag aggtgtcttt ctcagaggtg
     6421 taacaacatt ttcttctctt gatcctgacg cctcaagtag aacaaagatt tatccaacag
     6481 ataattttgg tgaaaaggga aatcaggtcg gctcattcca gggggatgat ttcaaaaagc
     6541 acgatcacaa atttcaggca ccaataacag gagaaaaggt tggatatgct tctgacagca
     6601 gtgtaagagc aaattttaaa gaaagtgcga ctttagttaa tggaggagaa gaaacgaggc
     6661 caaagaatgc ttatgtccat tatttaataa aagcagcaca tcaatcgttg gacttgcctc
     6721 aaatctttga gaacaatagt attgttgaat aaactgccgc caacctgtcc tttgcgttag
     6781 tgagcagtcg atacgatcca cgtaattgca tttaattaat taatgtggga attgtgcagg
     6841 tgtctgcatg tcccactcta tttcatttct gccatctttt tccgggcttc ggcgtccgcg
     6901 aaagtgaaag attgccacgg accatgctcc gctttcagca ggaaagaatt gcgccgcgct
     6961 tcgagatgat cggtgaattc atcgatgttg tgatagctga tccagtgaac tggatttttc
     7021 tggtgcgctt cgatctgcgt gcggatgaca tcgaggttat tgtaaacgat gggcaggaca
     7081 tcctcgtgcg cttcgaggta aaaatcgaca tcgatctgcg cgggattgaa cgcatggagg
     7141 ttgtagttca cgatcacctt gtcccagttg aatagcgcgg cgaaaaggat agctccgtaa
     7201 atgacggcgg aattcaggcg cagcaggaaa taggaagatt tcgtccgcac tattttccac
     7261 gcaatgctgg cgagcccggt catgacgagc gcaagaaaaa agaaaacgaa aattcttccg
     7321 taggcgagcc cgtgccaggt catgtaatga tagttgcgca ggaaaacgga gaacgtcagc
     7381 acggcgttct ggaacagcca cgccagcgcc agccgtttca gccacctgtt tcgcgaataa
     7441 aaatgaaggt tgccgcggaa gaaacgcagc gtgacgatca tggaaagaag gatgctgatg
     7501 gaaagtatcc aggtgccgtt gtgcacaaaa tccttcaggc tgaaatgttc cggcagcacg
     7561 aaatggatcc acactttttc tacatctgtc acattcacga ccagcagcag cgcattcagc
     7621 aggaccagtg tcaccagccc ggccagcgct tcttccttca gcgccgtctt tcggccgaaa
     7681 taaggaagtc gtttgcgccg gagatcgaac ggaagtcctt tgtcggttcg tatgcggttg
     7741 tacccgcgca gcaataaaat gatcagcgcc acgccgcagg cagctatgaa aaagaaatgc
     7801 gcgaaggaaa aattgcggaa gaacccctgg atgacgttga ggaaggaagc gttgagcgcg
     7861 aagaaatgcg gactccccca gagatagata aggtaaaacc cggttgcggt gagcagcggc
     7921 agcagcacga ccagcgcata gaagcgggtt ttcccgcggt acctgttgtt cttcgaaaat
     7981 tgttcacggt gatgcgcgaa cgtaagcgga aagtcgaaga tgctgaccag cgcctgcagc
     8041 gacgcatgga aaaaagaaac gagacctgct tcggaggcaa acatggaaaa catcagcagc
     8101 gagagaatgc agctgaacag cgcccagtga ctggcgtgaa gaaagatcat cagcgcgctg
     8161 agcaatgcgc ccccggctgt gacctgcacc gggcgtcttc gtaacgcgga cgggtatcgc
     8221 cggagaagga cggcgatcat caccacggaa aaaagaagca cgtttatccc cggcgtcgtg
     8281 tgccagaaaa gaagatcgga aagaaaaaga agaggaggga acaggacaat aaagcgattc
     8341 atggcggcgt ttttgactgc acattaacgg gccttcaagc agaaaagtat tggctgcggt
     8401 tttttttcac aattgtttgg aaacggtgaa gccggtacgt ccggccttct gttcaatcgc
     8461 tcccgggcgg aatgttttca taaattgaaa caggaataaa cccaaccttt gccgtgaatt
     8521 cttcgtttat aaaaaaaaca cgtcatgaaa aaaatgtacc tcgctcttac cctccagttg
     8581 ttttcggcag cgcttttttc acagcagtac tggcaatggg ggcatgaagc cggcggtgca
     8641 ggtaatgacg aggcgatggc cattgcgacg gatcccaacg ggtacagcta cgtcaccggg
     8701 tattttaatt caacggcttc gtttggtccg aattcactga cgagcagcgg cggtgcagat
     8761 attttcgtgg cgaaatattc tccgggagga acgtgcctgt gggtgcgtgg tttcggagga
     8821 gtgaacaacg acgcgggaca tggcattgcg ctggacgccg caggtaactg ttacgtaacc
     8881 ggcgatacgg gaatgtgtgc ggtggtgcgg aaatacaaca gcgccggtaa tgtgatctgg
     8941 acgactgttc aggcgaatgc agcttccgcc aattgcagca gccgggcaat aaccaccgac
     9001 agtgcgggaa attcctggat caccggctac agcggcgggg gagcacaact gttcggatcg
     9061 cacgtgctga acggtcccgg cggttacgtt gtgaaatttg atacggcggg cgtgcccgtt
     9121 tttgcaacga agctggcgac cgcgggggcg atcgacctgt acggcgttac gcatgatgcg
     9181 gcgggaaatt gttacgcaac cggctacctg cagggccagg acaatatcgg ttcgttgcag
     9241 ttcacgagca cgggcagcag ggacgcggtc ctgatcaaag tcgacacgtc gggaaatttt
     9301 gaatggctga agcagtcgct tgcgctgtcc ggcggaacgt cggttatgcc caatgggatc
     9361 tgcgctgata aaggagacgg aatttatttt tgcggcgatc tcgcggggaa aactgttttc
     9421 gggaatgata cgctgcccgg tgattccgca ggcggcagta acattttcat cgttaaatac
     9481 gatacggcag gcaccgcgct ctgggcggaa gagccgacgc tgacggcgca ggggcccggc
     9541 cagcttcacg cgtatgcagt ggcgaccgac acgtcaggca atgcctacat gaccggtgtt
     9601 ttttcttccg acctggtgtt cggatcgaca cccttgcttg acgggatact gggcatgaac
     9661 acatttatcg tgaagttcga accgaatgcg gggacatgcc agttcacgat ccagtccaca
     9721 ggctctaatc ccggtgcgtt cgggctgggc atcagcacgg atagttacgg tggcgtttac
     9781 attgcaggtt ttgataaagc tccggttgtg tttggttcga acacgaccaa tttcatcggc
     9841 ggggaagata ttttcgtcgc aaggatagcg gaaggaaaag agtccgggat aaacgaaacg
     9901 gatttttccg cgcaggtttc gttcttctct tccggcgatg ctttccagtt gtctgttgcc
     9961 gatgaaaaaa tgctgtacgt gcatccaaca cttaacattt acgacatgac aggccgcctc
    10021 gtgaaaacaa tcgctgtgaa tgagctgcag accaccatca gcagcgcaga cctcgctccc
    10081 ggaatttatt cctgggaact caccggttcg cagcagcgtt ttgcatcggg gaagacggcg
    10141 ctctgccggt gaaaaatgaa ttcgtgattt ttgtgccgtc gtcgcaaaaa aacagcatac
    10201 gaacactatg gcccgggacc tcgcggtgaa aatccgggcg gagcctttac cgaaaaccgg
    10261 ggtgaactaa aaagccggga aaactttcgt catcccggct tttgtttttc actcgctttc
    10321 gttcggtcag agcgccagct ttttcctgag gttcgtatcg atcgcgctga ggaaatcttc
    10381 cgtgttcagg taatgttccc cgagcttcac gttgttgccg tgtatgcaca ccgcaaggtc
    10441 tttcgtcatc ttgccgctct ccaccgtttc cacgcacact tcttcaagcg ctttcgcgaa
    10501 tttctgaagc tccgcgttgt tgtcgagctt gccgcggaaa tcgagtccgc gcgtccaggc
    10561 gaagatggaa gcgatcgggt tcgttgacgt cggttttccc gcctggtgat cgcggaagtg
    10621 gcgtgtcacc gttccgtgcg cagcttcggc ttccatgatc tttccgtcgg gtgtaacaag
    10681 cactgatgtc atcagcccga gcgagccgaa tccctgcgcg accgaatcgc tctgcacgtc
    10741 gccgtcgtag tttttgcacg cccagatgaa ttcgccgtgc catttcagcg ccgaagccac
    10801 catgtcgtcg atgaggcggt gctcatacac gatgccggcc gctttgaatt tgtcggcgta
    10861 ttctttttcg tacacttcct ggaaaatatc cttgaaacgc ccgtcgtatt ttttcaggat
    10921 cgtgttcttc gtggaaagat acaccggcca ttttttcatc aggcccatgt tcaggcacgc
    10981 atgtgcaaaa ccgcggatcg attcgtcggt gttgtacatc gtcagcgcga cgccgtctcc
    11041 tttgaagttg tacacttcga acgtctgcgc ttcgccgccg ccttcgggcg tgaacgtcat
    11101 cgtcagcttc cctttgcctt tgaaaacggc atccgtcgca cggtactggt cgccgaaagc
    11161 gtgacgtccc acgatgatcg gcttcgtcca gttcggcacg aggcgcggca cgtttttgca
    11221 gacgatcggc tcacggaaaa cggtcccgtc gaggatgttc cggatcgttc cgttcggcga
    11281 tttccacatc tgcttcaggt gaaattcttt tacgcgcgct tcatccggcg tgatcgtcgc
    11341 gcacttgatg ccgacatggt atttccggat cgcttccgcc gcgtcgatcg tcacctggtc
    11401 gttcgtctgg tcgcggtatt cgatgccgag atcgtaatac ttgatgtcga tgtcgaggta
    11461 gggcaggata agtttttcct tgatgaattt ccagataatg cgggtcattt cgtcaccgtc
    11521 gagctcaaca acgggatttg cgactttgat ctttgccata atgagtgtgt gattttaaga
    11581 ggtctgattt tggagagccg aaattaagta tttggaacgg agaatgggag ccgctgtcgc
    11641 gtgatttttt tgtgatccgt gcttttggcc gctcctgaaa aggaatttgt agtttagtgt
    11701 caaatccgcc cgtatcatgt cttcacccgt ttcgattacc gtcagaaccg tcatcagcgc
    11761 tcccgtttcg cgggtgtggg aatgttttac gcaaccgcag cacatcacgc ggtggaactt
    11821 tgccgtgcct gaatggcatt gccccgccgc ggaaaatgat cttcgtcccg gtggaaaatt
    11881 ttcctggcgc atggaagcca aagacggaag cttcggtttt gatttcgcgg gaacctacac
    11941 cgatgtgaga ccgaacgaac ttatcgcgta caccatcggc gacgggcgga aagttcacgt
    12001 cactttcatg acggacgggg attcaacgga agtcaccgaa accttcgaag ccgaatcgca
    12061 gaatccggtg gaaatgcagc gtggcggatg gcaggcgatc ctcgataatt tcaggaagca
    12121 cgccgaagcg ggctgaggag cgctcacaca ttcattgtgc ccaccggcag gtaggcgcgg
    12181 ctttccgtta ccatccttat gaattccgcg ggattcctgt tgtaaagtga ctgcaccacg
    12241 tgcgagaacc cgatgtgcgc atgcggcgtg taccgtttta cgccccggag aaacaggagc
    12301 tggtcttccg gtgaattgca cttgatcatg atcgcgagcc cgttgatgtc gagaagctgg
    12361 taataggatt cgtggtacag cgtcacgaca tagtaagccg tgtgctgcac gcgcaccggt
    12421 tttatccagg cgatcctttc cgggtgatgt accagctcgt cgatccagta atcttctcct
    12481 ttcaggtcgc cgcggaaacg atacacgacg agcgcgatcc cgcccagcgg gagcagcccg
    12541 agaataagaa tcggggcgaa agggattttt atcccgaaat agatgaagaa aatgctcagc
    12601 aggaaaagta ccacgatccc ggcaatcgcg ccgatcttgt ttttccagca ggtgtcgcgt
    12661 atgatgcgct cgaaatcaaa atctcttttt acaggcatga gggtggggtt aaggaatgga
    12721 aataaaaatt cccggataaa atacgaacaa tgcgttgacc ggccatccgg ggagctccgg
    12781 gtttccgcag gcggaatgaa aataaattgc atttcacgga aaagggagcc gctcatgaat
    12841 atgttttggt gcgcttacta cggaagctgc accgataaat tcggcgtgca gtggatgttc
    12901 aactgcaccg cgaaataaag aggtgtttcg ttcctgcccg gacgggaaat tttcgtttcc
    12961 cgttttttgt tttcttcggc atgtaaacca ccgctttccg ggtgagcgcg gtccggaacc
    13021 ggtgcaggga aaatgatgtt ctttcgtttt tcttcgtgaa aagcttcgtg ttcctgcggt
    13081 tgaaaacccg gcacattttc tgctatcttg ctgaagccag tccaaccgaa aatgagaaag
    13141 atccgcttcc tttttatctt cccgctgctc ctgtttccgt tttcttttct ccattcccag
    13201 cacgccgtca tcaccgtctg gaaagattcc gccatgggcg ccatctacaa caaagccacg
    13261 cagacggtcg cgtacgggaa gccggataaa aaaggcacgt acaaaattta cctctgcgat
    13321 accctcggca acaatgagca catgcttacg tacagcggct ggtcgcccga ccgtcaccag
    13381 tgggcggagg aatgggatcc ttcgggaaaa ttcctgtact gctatgtcga gaaaacagaa
    13441 tacgtgaagg agaaaggcca caagcgcaaa ccggtcgatg catgtcccgg ctacggcgcg
    13501 tacactgacc tttgggcgat cacgcgcgac gggaagaaag cctggaagct cgtggatgag
    13561 ccgaacagct acaacagcgg catcatccat tccgccattt cacccgacgg aaaaatgttc
    13621 gcgtggagcg agcggatcaa agcgccgcaa ttcaacatga acctggcggc tgggtcttac
    13681 gtcatccgcg tggcggacat cacggtggat tcctgcccgc atttttccaa catccgcacg
    13741 ttccagcccg ggggcataga cgccacaaac gaactcgacg gcatctgccc cggcgattcc
    13801 gtgatcacgt tttattctac gttcgaatcg aaaaatcttt ttgcaacacc ggtttataaa
    13861 atcaatattc tcaccgggaa gatcacgaag ctcaccaccg ccagcttcgc gcaggccggc
    13921 acgttcacac ccgacggaaa gcacattgtg tacatgacgg gcgacagctg caatattttt
    13981 cccccgcaac cgcagggagc cgactggtgg atcatgaata cggacggaag cggaaaaaga
    14041 agactgacat acatgaacaa aaaaggcgat gcgcaatgcg ataaccatta ccgtcttgcc
    14101 ggaacggttt cattcatgtc ggataattgt tttttcggcg gtgtaatgac aaaatcactc
    14161 gggctggtgg gctatacggt gaaagtgagg tttgaatgaa atgaggaatg atcgtacgtc
    14221 actcctgcgg ctgaaagaag tccgcgcctt tgcggttaac tttcccttcg gtctccaatc
    14281 attttcattc taactttgcc tgcctgtaat ttcatcatgg aaaaactcat tgttaccaat
    14341 actgtcgcca tcagcgcgac cccgcagcgc gtgtgggacg tcctcaccaa accggaagaa
    14401 actaaaaaat acatgttcgg ctgcgaaact gtttccgact ggaaagaagg aagcgcactc
    14461 ctctggaaag gtgaatacga aggaaaagaa atggtgttcg tttccggttt tattctcgaa
    14521 atcgttcccg ggaaattatt acgctattct gtgatcgatc ctaatgcagc gtacccgcag
    14581 actcccgaaa atcatttacg cgtgacctac gaaatcacac cgggcaacgg aagcgttacg
    14641 ctcacggtca cccaggatgg tttcgaaggc gccgcggaag gagaaaaacg ctaccgcgat
    14701 acgtacaaca acggtgacgg ctggaacccg gtcctgcagg caatcaggaa agtggcggag
    14761 gaacgttgac cccggactca tccctattta gtacatttgt tttccttaaa aagcagaaga
    14821 aattatgaga accatcgcct tccgtgaagc actccgcgaa gcgctgtcag aagagatgcg
    14881 gcgcgacgaa aaagtttttc ttatcggtga agaagtggcg gaatataatg gcgcctataa
    14941 agtgagccag ggaatgctcg ccgaattcgg tgaaaaacgt gtgatcgata caccgatcgc
    15001 cgagctgggc tttgccggta tcgcagtggg tgcggccatg aacggactgc gcccggtggt
    15061 cgagttcatg acattcaact tttcactcgt cgccatcgac caggtgatca actctgcttc
    15121 gaaaatgctg agcatgtccg gcggacagtt caatatcccg atggttttcc gtggtcccac
    15181 cggcaatgcg ggacagctgg cggcgcagca ttcgcaggcg ttcgaaagct ggtatgcgaa
    15241 ttgtcccggt cttaaagtcg tcgtgcctgc aaatccttac gacgcgaaag gcctgctcaa
    15301 atccgcaatc cgcgataacg atcccgttat tttcatggaa tccgagctga tgtacggcga
    15361 caaaggcgag gtacccgaaa gcgaatacct tcttcccatc ggcgtggccg atatcaagcg
    15421 cgaaggaacc gatgtgacga tcgtttcttt cgggaagatc ctgaaagtgg cgcttgccgc
    15481 tgcggctgag ctggagaaag aaaatatttc cgccgaagtg atcgatctgc gttccgtgcg
    15541 ccccatcgat tacgcaacgg tcattcgttc tgtgaagaaa acgaaccgcc ttgttatcgt
    15601 cgaagaagcg tggccgctcg cgaatatttc caccgatctc acgtatcacg tgcagcatca
    15661 cgcattcgat tacctcgatg cgcctgtgcg ccgtgtcgtc agtgcggacg ttccccttcc
    15721 gtatgcgcct acactcatcg aggtcgcgct tcccggcatc gcccaaacgg tgaaagccgt
    15781 gaaggacgtg atgtacgttt cctgagtttt tgcttcgttg cccgggattt taaatcgtct
    15841 ccgcctgacg gttttccttt cgttgttttt ctgtgaacct ccgtgaaaca acctctgcgt
    15901 cctgcacggt aaaaaagagt ttacctccaa agccgtcttt gaaactgaag cccctttttc
    15961 tttcgtgcac tttgtgaaaa cgctttgtgt tcttcgtggt taaaacgact tcagttcatc
    16021 gcggtcatcc gttgctctga cctttcaacc ttccaacttt gaacttgaaa cctggccaag
    16081 aataattttc cttccgcata ttttttgaca cgatccgggc gttataaata caggtcgcgg
    16141 taatgatgac cttgcagccg gcaactcccc gccgacgttt tttttatcag cctgtttttc
    16201 tccatatttg aaaatgcgcg tcccttcctg catattgatt ttcgcttttg ttttttcact
    16261 cgcgaaactt tcagcgcaga gtttttgcgg ttatgagcac agcaattacg ctggtattgt
    16321 cggtgcgtcc tacaacccgg cttcgctggc cgacaacaac tacagcctgg atattcttct
    16381 tttcggtttc gacgtcgaag gcgcgaacaa ttatgtcggc gtgaagcgga aagacatgtt
    16441 ccacccggac ttcggcccgg agcatttctg gctgcgcgac cgcccgttta agaaatcagt
    16501 tttcttccgc aatgaaatcc tgcttcccgg catcatgttc tccaatgaaa aatacggctg
    16561 agggatcgac atgaaagtgc gcacgtattt caacgtcgac ggaattcagc cgccgcttgc
    16621 acacctgctc gccgaagggt tcaccgatcc gtccgactat aataccggat accacaaccg
    16681 gcacatcggc atcaatgaat gtacctggtt cgagatcggc ggaacgtatg cgaaaaccat
    16741 ctggaccggt gccgaccgtt tcgtaagcat cggtgttcgt ccgaaattcc tgctcggcct
    16801 cgccgccgct tatgtattta tcgacaatgc cgattatgaa ttttacaacg attccgtcat
    16861 ggcgcttttc ggcggtaacc tgaagttcgg tcattcggac aacttcagct tcaacgaaaa
    16921 tttccagcct tcgtaccgca tcgggttcaa tcccggcatt ggcctcgacg cagggatcat
    16981 ttacgagttc cgtcccggcg acatgcagaa aaatgaaaag cccggaaaag agtggcccgg
    17041 ctttcgcgat cgtccggtgt ataaataccg tatcggcgca tcgctgacgg atctcggcat
    17101 cattcatttc cgttcgggcg cgtttaccga tcagtattcc gtttcttctg atgcatggaa
    17161 tgtcgcccac gatgttttca atgcaacggc gcccgctccg ctgttcaata atttcaacct
    17221 gcagtatcac ggcgccggcg aaggacaatc attctggatg cgcacaccgc tcgccctcgg
    17281 tcttttttac gattaccgct tccgcgaaaa tttatttttc agcgcgcagt ctttcaccgg
    17341 actttacctc cgcggaacgg atttcaaaaa agtccacgag cttacgcgca tcagtttgac
    17401 ggcgcgatac gagagaagat ggtacggcgc ctgggtgccc gtttcattca cgagaatggg
    17461 aacgttcagc atgggtgcgg gattgcgcct cggcccggtc atcatcggca cgacggatat
    17521 tctcaacctt actatgaaga acaagcgcat ctacaacgcg gatatttatt ttgcgctgaa
    17581 agttccgttg tttccgttgg gaagcggtag aaagaagaaa acaaaggtca ggaaaacaac
    17641 ggacgttgat aaatgtcccg ggtagcccgc actttttcgt aaagccctgc agctctttct
    17701 gaaaaaccgt ctatcccggt ttctgaagat tcggccgcga atttttcacc cggtcgtttg
    17761 gagtggaaga gaagacgcga cgtgaaacgc tcttttagcc cggctgccgg ctccgcgccg
    17821 cactgacgca gcaggtgaaa agcggcgttc tcaataccgt ctgcgtaatc tgcgctgaaa
    17881 aaattcccgt ccgtttcctt tgccctgaga aaacattcaa aaaaacgctc cagcattttt
    17941 cctgcataga aacggtggtt cgtcagcatt tcttccgtca gctcaattcc cgtcagggaa
    18001 gacggaagca ggccggggat catctgcatc cctgcctgcc gggcatggga acggataact
    18061 tcagcggggt gccggtaaag gaaaacaaaa ggcgtgcggg gataaagctt ccgcagcaca
    18121 gcgtaatgga aaatatgcca gctgtcgagc ttgatgaacg ctgaattttc ttccggattt
    18181 tttttcgcgc acatcagttt gatgctggcc gtgagcaatt gttccgcgga aatatctccg
    18241 aagtttttct tttcatgcat gcgaaggagc aggtcgtcga aaagcgggac ttcaggcagg
    18301 acgatattcg caggatcttc actgagcagt tgcgaaagca atgtcgaccc gcagcgcgat
    18361 acgtgaaaaa taaatgcggc cggttctgca gcgttcacat tttgagaagc gcttaccagc
    18421 gaagcggcgg aagaacgatt ctcccggaac gagctgtttt cttccagcga gagacgacga
    18481 tggatcgttt cttcgaaaaa aggttcgtca aattttttgt ttccgagata gatccagtgg
    18541 aaatttccgt ctgcagccgg tttccaggga aaccatccgc gcggagccga ataaaagtcc
    18601 aacttgccgc tcctcatttt tcggaaagac cggacaaaag tttttcgagg atatcgcggc
    18661 tggccggcgt atcgatgcgt gaaagctcct cgatcatgcg ccgtttcgtt tcatcgtcgg
    18721 gcgcaagcgc tttcttttcc gcttcgaaat cgtagcccgc gttggcaaaa agttcgtcgg
    18781 accattcatt gcgcacgcag tcgatcacga gatgcacgcg ttccgttgca ccgtgatttt
    18841 ttacgctgtg cggaaggtcg aagttcgcgt accagcattc tccctctttc atttcgatgc
    18901 gctctccgcc gacgataaaa tcgatctgcg gacccgtgac gaccggaacg tgcacgcgaa
    18961 aatttccgaa agggtaggcg agacgtatgt cgcggtgcgt gcgtatttcg ctgcccggtg
    19021 caagacggag cagccgtacc gcttcttttt cgcagcggaa ggaatcgacc accgcactga
    19081 aataa
//
LOCUS       DASTSF010000094         3153 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1951606,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000094 DASTSF010000000
VERSION     DASTSF010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3153
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1951606"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(97..1911)
                     /gene="rpsA"
                     /locus_tag="VFU15_02900"
     CDS             complement(97..1911)
                     /gene="rpsA"
                     /locus_tag="VFU15_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010665027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEU4716748.1"
                     /translation="MAETQTETVATPEFDWNAIGKKQDIYSKEELAKLESLYGQTLKS
                     INDHEVLEGIVVAKNNKEVVVNIGYKSDGVIPLSEFRYNTDLKVGDKVEVYVESQEDK
                     NGQLVLSHKKARAMKSWERVNNALANDEVIKGFVKCRTKGGLIVDVFGIEAFLPGSQI
                     DVKPIRDYDIYVGKTMEFKVVKINNEFKNVVVSHKALIEEELEAQKREIISKLEKGQV
                     LEGTVKNITSYGVFVDLGGVDGLIHITDLSWGRISHPEEIVKLDQKINVVILDFDDQK
                     KRIALGLKQLSPHPWDNLSADIKVGDKVKGRVVVIADYGAFVEIQPGVEGLIHVSEMS
                     WSQHLRSAQDFLKVGDEVEAVVLTLDREERKMSLGIKQLTPDPWQEILTKYPKGSRHT
                     AKVRNFTNFGIFVELEEGIDGLIHISDLSWSKKIKHPSEFTKIGENIDVVVLDVDVEN
                     RRLSLGHKQLEENPWDVFETLFTVGSVHKGTIISANDKGAVVQLPYGVEAFCPNKHLL
                     KAEGGNAKVDESLDFKVLEFNKDAKKIIVSHRNTVESIAIEEKDMKGGKKDSKGNDET
                     KKAVKKVKESLEKTTLGDVSSLADLKSALEDKEQGQKD"
     gene            2295..>3153
                     /locus_tag="VFU15_02905"
     CDS             2295..>3153
                     /locus_tag="VFU15_02905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716749.1"
                     /translation="MKKGLLLVLGASAAFSLFAQAPQTPKIAGCRMADLNLKKAGNPD
                     NSGNFFASASAMDRHPNQVAPLAEGTMFTSSYNAFTLLVSQSHMLYANQALSAVLFTH
                     RMSRDWPADANVGSGYIEYSWTTDFGATWDSSYYQDQAVLANKAFRYPTGAILNPTGN
                     TSVANAYSVSTGPWTNSATSGNSWLGYYANYQKMMNGATMNTTVFTVSTPGSAIQYFP
                     RIGSQVVNDSTYWVTSSLYNDPASTTNPGLRGAGLMKGTYSMGNMVWTFDSIKPSVHH
                     DGSGNADMYS"
BASE COUNT          728 a          841 c          703 g          881 t
ORIGIN      
        1 aaaatccccc cgaaaaaatt tccgggggga aaactaaaaa ccctggcgta gtttttagtg
       61 acctggcgga ggcaccgtcc aggtttatct taggtgtcag tctttctgac cctgttcctt
      121 gtcctcaagc gcagatttga gatccgcaag cgaagaaacg tcaccgagcg ttgttttctc
      181 gaggctttcc ttcactttct tgacggcttt cttggtctcg tcatttcctt tcgaatcttt
      241 cttgccgccc ttcatatctt tctcttcaat ggcgatcgac tcgaccgtat tgcggtggga
      301 aacgatgatc tttttcgcgt ccttgttgaa ttcgagaact ttgaagtcga gcgattcgtc
      361 gactttggca ttcccgcctt cagccttcag caaatgcttg ttcgggcaga aagcttcaac
      421 accgtaggga agctgaacga ctgcgccttt atcattggcg ctgatgatcg tccctttgtg
      481 tacggagcca accgtaaaca aagtttcaaa aacgtcccac gggttttcct caagttgttt
      541 atgaccgagg ctcaggcggc ggttttcaac gtccacatca agcacaacca cgtcgatgtt
      601 ctcaccgatc ttggtgaatt cggacggatg cttgatcttt ttggaccagc tcagatccga
      661 aatgtggata agtccgtcga taccttcttc caactccacg aaaattccga agttggtgaa
      721 gttccgcact ttcgccgtgt ggcgtgaacc tttcgggtac tttgtgagga tttcctgcca
      781 cggatcaggc gtaagctgct tgatgccgag cgacattttg cgctcctcgc ggtcaagtgt
      841 aagcaccaca gcttccacct cgtcgcccac tttcaggaag tcctgtgcgc tgcgcaggtg
      901 ctgcgaccac gacatttcag aaacgtggat cagtccttca acaccaggct ggatctcgac
      961 gaatgcaccg taatccgcaa ttacgacaac gcgtcctttc accttgtcgc caaccttgat
     1021 atcagcactg aggttatccc acgggtgcgg agaaagctgc ttgagaccga gtgcaatgcg
     1081 ttttttctga tcgtcgaaat cgagaataac gacgttgatc ttctggtcga gtttgacgat
     1141 ctcttcggga tgcgaaatac ggccccagga aagatcggta atgtgaataa gtccgtccac
     1201 accgcccagg tccacaaaca cgccgtagct ggtgatgttt ttgacagttc cttcgagaac
     1261 ctgccctttt tcgagcttgg aaatgatctc gcgcttctgg gcttcgagtt cttcctcgat
     1321 aagcgctttg tgagaaacaa cgacgttctt gaattcattg ttgatcttga cgaccttgaa
     1381 ttccatcgtt ttgcccacat aaatatcata gtcacggatg ggttttacgt cgatctggga
     1441 gccgggcagg aacgcctcga ttccgaatac atcgacgatg agaccacctt tcgtgcggca
     1501 tttaacgaag cctttgatca cttcgtcatt ggcaagcgca ttgttcacgc gctcccatga
     1561 tttcatcgca cgggcttttt tatgagaaag caccagctga ccgttcttgt cttcctggct
     1621 ttccacgtaa acttcgacct tgtcaccgac tttcaggtcc gtattgtaac ggaactctga
     1681 cagtggaata actccgtctg atttgtaacc gatgttaacg acaacttctt tgttgttttt
     1741 tgcaacaaca attccttcaa gcacttcatg atcattgata gactttaatg tctgaccata
     1801 gagcgattcg agttttgcaa gctcttcttt cgaataaata tcctgcttct ttccgattgc
     1861 attccagtcg aattccgggg ttgcaacagt ttctgtttga gtttctgcca tctggcttaa
     1921 attaatctgc tttcgcatgg tcctttgccg gcatcaaagg acttgttcca catgcgggct
     1981 gccggacccg cgtttttttg agaaagggct acaaagttat gtttttagca gcgtccctga
     2041 caggaataac cggcttaatt tttatttttc tgcctgccac tttttcaggc aaaaacaagc
     2101 ccttaataat caacaaatta agccctacca gggacaaccc gccgaatata atcgttaccc
     2161 gaccggatta tgttttgcga atattcattt actattaaaa ccttgcaaat cattttttgt
     2221 ccgttataca caatttccct atttttgccc gtttcgtttc cgaattcatt aactgtaaaa
     2281 acaaaccaaa acaaatgaaa aaaggattac ttcttgtcct cggcgcatcg gcggcattca
     2341 gccttttcgc tcaggctccg cagacgccta aaattgcagg ctgcaggatg gccgatctca
     2401 acctgaaaaa ggctgggaat cctgacaatt ccggcaattt cttcgcttca gccagcgcaa
     2461 tggatcgcca cccgaaccag gttgccccgc ttgctgaggg aactatgttc accagctctt
     2521 acaacgcctt cacgcttctt gtttcccagt ctcacatgct ttacgctaac caggcgctca
     2581 gtgctgttct ctttacgcac cgtatgtccc gcgactggcc ggctgatgca aacgtaggaa
     2641 gcggctacat cgagtattcc tggacgaccg acttcggtgc tacctgggat tcgagctact
     2701 accaggacca ggcggttctg gccaacaaag cgttccgcta tcctaccggc gctatcctga
     2761 acccgaccgg aaacaccagt gttgccaacg catactctgt ttcaacaggt ccgtggacaa
     2821 actcggctac ctctggtaac tcctggctgg gttattacgc gaactaccag aaaatgatga
     2881 acggagctac gatgaatacc accgtattca cggtttcaac tccgggatcc gctattcagt
     2941 atttcccgcg tatcggcagc caggttgtta acgacagcac atactgggtg acctcttctc
     3001 tttacaatga tccggcctct acaaccaacc cgggccttcg cggggccggt cttatgaaag
     3061 gcacctattc aatgggtaac atggtttgga cattcgattc tatcaagccg agcgttcacc
     3121 atgacggcag tggaaatgca gatatgtaca gca
//
LOCUS       DASTSF010000095         2558 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_163862,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000095 DASTSF010000000
VERSION     DASTSF010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2558
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163862"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            212..1087
                     /locus_tag="VFU15_02910"
     CDS             212..1087
                     /locus_tag="VFU15_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014202044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU4716750.1"
                     /translation="MKKNLFYAAAVIALTACGRFSNTSDPAKDKQVVRIVCVSKQYNE
                     IIYALGAERNLVAVDLSSTYPDAIKSLPNVGYHRMLSAEGIISMNPTVVMHDNNVGPD
                     NVLRQLDQLKIPIKTFGKAETIEETKGLFREMGEYFGRKVQADSLCKVLTDDMQAAVE
                     AKEKLGGDTMIKVVVIHFGRIGNSYLIVGKHSITTNLLSMAGAQNPVEGEKGMMPLSA
                     ETIAAANPDVILVTDYGYDRLGSVDAVKTLPGVATTNAAKNNRIYRVEEHDMMYYGPR
                     TGKNIIQLIQLIHQK"
     gene            1090..2115
                     /locus_tag="VFU15_02915"
     CDS             1090..2115
                     /locus_tag="VFU15_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014202045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEU4716751.1"
                     /translation="MGKRTVYSILWIAFVLTFFISFIFGAITISWKDMYGTVVHLFSG
                     TISQSSLDERIFFYIRMPRVILCCIAGAALSAAGAVTQALFRNPIVEPGLIGTSGGAA
                     FGAALLFVFGSAMKWTPGEWTMPIAAFCGGAFATWLVYLLASAGTKKQSVTTLLLCGI
                     AVNAMFMSGVGFLSYLARDPQARSITFWNLGTLSGASWTAVFIVGIICTIAMIFSLRK
                     AKDLQAMLLGEEEATMLGVNTRRLKLQVSFLVVLMVAGVTAFTGVISFVGLVVPHLVR
                     RMNISNTRSVIFGSALAGAILLNLADIIARTSLAPAEIPIGIITSIVGAPFFLYLVRR
                     STDFFGE"
     gene            2118..>2558
                     /locus_tag="VFU15_02920"
     CDS             2118..>2558
                     /locus_tag="VFU15_02920"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU4716752.1"
                     /translation="MLELKNISHRIGNKMLLDNITASFLPGRLNVLIGPNGAGKSTLL
                     KIAGLQLFPVSGTVSYDEKIFTSRDRESLSVIRAFLSQQTEIPFPIRVKEVVMMGRHP
                     HFSGTPSPYDRKIVNEAMDYFKARHLADRLYNSLSGGEKQRIHFA"
BASE COUNT          651 a          619 c          616 g          672 t
ORIGIN      
        1 atttccggcg gaagcggaga tggctatatt ctccccgctc cctatagcgc tacattttac
       61 ggcggactga aagtaagtgt gaacctggat aagaagaaat aaacggtaaa gaatacccgg
      121 gcgaagaact ggtggttttt ccggtacgac ttccctgccg caaaacgaag tggttcccgg
      181 ttcttccccg ggtattaaaa aaaatactgc tatgaaaaaa aacctttttt acgccgctgc
      241 ggttatcgcg cttacggcgt gcggacgatt ctccaatacc agtgatcctg caaaggataa
      301 acaggtcgtg agaattgtct gcgtttccaa acaatacaac gagattattt atgcactggg
      361 tgcagaacgg aacctcgttg cggtcgatct ttcgagcaca tatcctgatg cgatcaaatc
      421 acttcccaat gtcggttatc accgtatgtt gagtgccgaa ggaattattt ccatgaatcc
      481 gactgttgta atgcatgata ataatgtcgg gcctgataat gtacttcgcc agctcgacca
      541 gctgaaaatc ccgatcaaga catttggaaa agcagaaacc attgaagaga cgaaaggcct
      601 gttccgcgaa atgggagaat atttcggaag aaaagtgcag gccgattcgc tctgcaaagt
      661 gctcaccgat gatatgcagg ccgctgttga agcgaaagaa aaacttggtg gcgataccat
      721 gatcaaagtg gttgtcattc acttcggaag gatcggcaac agctacctca ttgtcggcaa
      781 gcacagtata acaacaaact tactttccat ggcaggcgca caaaacccgg tcgaaggtga
      841 aaaaggaatg atgccgctct ctgccgaaac aattgccgct gcaaaccccg atgtgattct
      901 tgtgactgat tacggttatg atcgcctcgg ctctgttgat gcggtgaaaa cgctgccggg
      961 tgttgcaact acgaatgctg caaagaacaa ccgcatttat cgtgtcgaag aacatgacat
     1021 gatgtattac gggccacgca ccggcaaaaa cattattcag ctcattcaat tgatccacca
     1081 gaaataatca tgggtaaacg cacagtttac agtatcctct ggatcgcctt tgtacttaca
     1141 tttttcattt cattcatttt tggtgcaatc accatctcat ggaaagatat gtacggcaca
     1201 gttgttcatt tgttttccgg gaccatcagt caatcttcac tcgacgaacg gatatttttt
     1261 tatatccgta tgccgcgcgt tatcctgtgc tgcatagcag gagcagcgct ttctgcggca
     1321 ggagcagtaa cccaggcgtt attccgtaat ccgatcgttg agccggggct tatcggaact
     1381 tcaggtggtg cagcattcgg cgcggcattg ctttttgtgt tcggttccgc catgaaatgg
     1441 acgcccggtg aatggaccat gccgattgct gcattttgcg gaggagcatt tgccacctgg
     1501 cttgtttacc tgcttgcttc cgccggaaca aaaaaacaat cggtaacaac gttgcttctc
     1561 tgcggtatcg ctgtcaatgc aatgttcatg agtggtgtcg gttttctctc gtatctcgcc
     1621 cgcgatccgc aggcacgttc catcactttc tggaatcttg gaacgctttc cggtgcaagc
     1681 tggacagccg tattcattgt cggtatcatt tgcacgattg ccatgatctt cagtttgcga
     1741 aaagcaaagg atctgcaggc catgttgctc ggagaagaag aagcaaccat gctgggtgta
     1801 aacacgcgcc ggctgaaact gcaggtaagc tttcttgttg ttctcatggt cgccggtgtt
     1861 acagcattta caggcgtcat tagttttgtc gggcttgtcg ttccgcacct cgtcaggaga
     1921 atgaatatca gcaatacgag atcagtgatt ttcggcagcg cacttgccgg cgctatcctt
     1981 ctgaacctcg ccgacatcat tgcccgaact tctcttgctc ctgcagaaat accaatcggg
     2041 atcattactt caattgtcgg cgcgccgttc tttctttatc tcgtacgccg gtcaacggat
     2101 tttttcgggg aataaacatg cttgaactga aaaatatcag tcaccgcatc ggcaacaaaa
     2161 tgttgctgga taatattact gcgtctttcc ttcccggcag attgaacgta ttgatcgggc
     2221 cgaatggagc gggcaaatca acgttgctga aaatcgccgg tctgcagctt ttccctgtca
     2281 gcggaactgt cagttatgac gaaaagattt tcacctcgcg cgatcgtgaa tctttgtccg
     2341 tcattcgcgc attcctgtcg caacaaaccg aaattccatt cccgatccgt gtgaaagaag
     2401 tagtaatgat gggcaggcat ccgcattttt ccggaacgcc atcaccttat gaccgcaaga
     2461 tcgtgaatga agcgatggac tatttcaaag ccaggcacct tgcggatcgc ctgtacaatt
     2521 cactgagcgg aggggaaaaa cagcgcattc actttgca
//
LOCUS       DASTSF010000096         3854 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1322264,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000096 DASTSF010000000
VERSION     DASTSF010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3854
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1322264"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            59..631
                     /locus_tag="VFU15_02925"
     CDS             59..631
                     /locus_tag="VFU15_02925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716753.1"
                     /translation="MSFAGNIKKKLAERRIRSEAALLRREKTAFNIAEAKTIGIVYLF
                     TGSEEFELLKKYVSYLRELKKKVKVVVYYHGKQEPYVQYSKIDYDFFGVKSHNWYGKP
                     SDHILKNFMEEPFDILIDINFTGEPVLTFLSALSMAKFKIGLYEDDNYIHDMQIESSA
                     GKGLKFFLRQVDTYLMKINTPMNDGATGAA"
     gene            664..2670
                     /locus_tag="VFU15_02930"
     CDS             664..2670
                     /locus_tag="VFU15_02930"
                     /inference="COORDINATES: protein motif:HMM:NF019318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HEU4716754.1"
                     /translation="MNRFFSFIRDRHSLLYKAFLFLSSLALIVYLYPKGFRFRYDPES
                     LKNRPWPYESLIAPFDFAIQKSPDELASERKELTEKFVPYFRKDENAEKQLLEKFSAE
                     FGSRPAILSAVKDVLSGGIRAITNGAGKGPEDEVLVVTGKVEEEHYLGEYADPVSADS
                     LVRKKLNAADAKLWDTKQTAFSATVVYDSSLTKQSLQQVLDNISPTRGAISKGQDIID
                     RGEIVDEANYQELESFRQEFLSLHAGGSNWALLFGQTGLAAFCLFILFQFLRLFRREI
                     LGNDPKLLFILLMIVLTVCMSYIPSVVENVPVLALPFCILPVIMRAFFDTRIALFVHL
                     ITTVIITISLPADRYSFLVIQLLAGITAIFSIVNMRTRAQLFYSVIAILCVYVMLHLA
                     ITAIQEGEMQSIRMNELVNYAVSALLVLLAYPLIFFFEKLFGFVSDVTLLELGDTNSP
                     LLRELAERAPGTFQHSIQVANLAEEAIRRVGGNSLLVRTGAMYHDIGKGDMPMYFIEN
                     QATGVNPHDELTFEESAAIIISHVIKGIEKAKAAKLPDIVIDFIRTHHGTTNTSYFLS
                     LYKRNNPEEFIDEELFRYPGPIPYSKETAVLMMADSVEASSRSLKKYDGESIDGLVDR
                     IIDHQADQHQFDNSDITMKDISAIKKIFKKKLRSIYHVRVEYPK"
     gene            complement(2719..3252)
                     /locus_tag="VFU15_02935"
     CDS             complement(2719..3252)
                     /locus_tag="VFU15_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002999808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEU4716755.1"
                     /translation="MPAKIRLQRHGKKASAFFHIVVADSRAPRDGKFIAKIGTYNPNT
                     NPATIELDNNTAFEWLQKGAQPTDTCRAILSYKGVLYRQHLHKGVLKGALTQEQADAK
                     LAKWMEEKQAKIQSKIDRLSGEMTSAAKKKMAEETAAKESKAAKVAAKKAAAVEAPAA
                     ETPAETTETAENTENNA"
     gene            complement(3419..>3854)
                     /locus_tag="VFU15_02940"
     CDS             complement(3419..>3854)
                     /locus_tag="VFU15_02940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716756.1"
                     /translation="TENADNPDGNIYTLLELPKYIGTGNYHNYGIAITGPADPDKETR
                     PVRIDTNLNYEWPQISGTKDAPEPMPLILTITVSGLTPGTTYNLYKYCDENDVPVRNF
                     NADAKAKNNPPWQVIKNPPGSTWGTSHPIQSNEKFFLRCVPA"
BASE COUNT          881 a          965 c         1065 g          943 t
ORIGIN      
        1 gaaaagctgg gcgtagcgat tatttccgaa gaggaattca tgaaaatgat ccgctgaatt
       61 gagttttgcc ggcaacataa agaagaagct cgccgaacgg aggatacgtt ccgaagcggc
      121 gttgctgcga agggaaaaaa cggctttcaa tatcgcggaa gcgaaaacca tcggcatcgt
      181 ttaccttttt accggcagtg aagaattcga gctgctgaaa aaatatgttt cctacctgcg
      241 tgagctgaag aaaaaagtga aggtggtggt gtattaccac ggaaagcagg agccgtacgt
      301 gcagtattcc aagatcgatt acgatttttt cggtgtgaag tcgcacaact ggtacggaaa
      361 accgtcggat catatcctga aaaatttcat ggaggagccg ttcgatattc ttatcgatat
      421 caattttacc ggcgagcccg tcctgacatt tctttctgcg ctttccatgg cgaaattcaa
      481 gatcggtctg tacgaagacg ataattacat tcatgacatg cagattgaaa gcagcgccgg
      541 gaaaggattg aaatttttcc tgcggcaggt ggatacgtac ctgatgaaga tcaacacgcc
      601 gatgaacgac ggggcaacag gcgcggcatg acaagaaaaa cgatcattat tgacagctat
      661 tgaatgaacc gatttttctc ctttatccgc gaccggcact cgcttcttta caaagccttt
      721 ctcttccttt ccagccttgc gctgatcgtt tatctctacc cgaagggatt ccggttccgt
      781 tatgaccccg aaagcctgaa gaaccggcca tggccttatg aaagcctcat cgcaccgttc
      841 gatttcgcaa tacagaaatc gccggatgag ctggcctccg aacgtaaaga gctgacggaa
      901 aaatttgttc cgtatttccg gaaggatgaa aatgcggaga aacaattgct ggaaaaattt
      961 tcggcggagt tcggcagcag gccggctatc ctgtcggcgg tgaaagatgt cctttccggg
     1021 ggaatacgtg ccatcaccaa cggcgccggg aaaggtccgg aagatgaagt gctcgtggtg
     1081 acgggaaaag tggaggagga acattacctc ggggaatacg ccgacccggt gtcggcggat
     1141 tcgctggtgc ggaagaagct gaacgcggcg gatgcgaaat tgtgggatac gaagcagacg
     1201 gcattttccg cgacggtggt gtacgacagt tcgctgacga agcaatcgct gcagcaggtg
     1261 ctggacaata tttcaccgac acgcggcgcg atcagcaagg ggcaggatat tatcgaccgc
     1321 ggcgagatcg tcgatgaagc gaattaccag gagctggaaa gtttccggca ggaatttctt
     1381 tcgctgcatg ccggcggcag caactgggcg ctgctgttcg ggcagaccgg gcttgccgca
     1441 ttctgccttt ttatcctttt ccagttcctg cgcctcttca ggagagaaat actcgggaac
     1501 gacccgaagc tgctcttcat cctgctgatg atcgtgctca ccgtgtgcat gtcgtatatt
     1561 ccgtctgtcg tggaaaatgt gcccgtgctc gcactgcctt tctgcatcct tccggtgatc
     1621 atgcgtgcat tcttcgatac gcgcatcgct ctttttgttc acctgatcac cacggtgatc
     1681 attacgatca gtctgccggc cgaccgctac tcatttctcg tgatccagct gctggccggc
     1741 atcacggcca ttttcagcat cgtgaacatg cgcacacgcg cacagctttt ttattcggtg
     1801 atcgccattc tttgcgttta cgtcatgctt catcttgcga tcaccgccat acaggaaggc
     1861 gagatgcaga gcatccggat gaacgagctg gtgaattatg cggtgagcgc gttgctggtc
     1921 cttctcgctt acccgctgat ctttttcttt gagaaattat tcggctttgt gtcggatgtg
     1981 acactgctcg aactgggtga tacgaacagc ccgctgcttc gggagctggc ggaaagagcg
     2041 cccggcacat tccagcacag catacaggtg gcgaacctcg cggaagaagc gatacgccgc
     2101 gtgggcggaa attcgctgct tgtgcgcaca ggagcgatgt accacgatat cgggaagggc
     2161 gacatgccga tgtatttcat cgagaaccag gcgaccggcg ttaacccgca cgatgagctg
     2221 acattcgagg aaagcgccgc gatcatcatc agtcacgtca tcaaagggat cgagaaggcg
     2281 aaagcggcaa agctgcccga catcgtgatc gactttatcc gcacgcacca cggaacgacg
     2341 aacacttcgt atttcctctc gctctacaag cgaaacaacc ccgaagaatt catcgacgag
     2401 gaactgttcc gttacccggg acctattccg tattccaagg aaacggccgt gctgatgatg
     2461 gcggattcgg tggaagcgtc ttcacgcagc ctgaagaagt acgacggcga aagcattgac
     2521 gggctggtgg accgcataat cgatcaccag gcggaccagc accagttcga caactccgac
     2581 attacgatga aggacatttc ggcgatcaaa aagatattca agaaaaagct gcggagcatt
     2641 taccacgtcc gcgtggaata tcccaaatga aaaaagcccc gctaccgggg cttttcgtta
     2701 ttgttcagcg ctgaaaactt acgcgttgtt ttccgtattc tctgctgtct ccgtggtttc
     2761 tgcaggagtt tcagcagccg gagcttcaac ggcagcggct ttcttcgcag ccactttcgc
     2821 tgctttggat tctttcgcag ctgtttcttc agccattttc tttttggcag cgctggtcat
     2881 ttcgccggag agacggtcga tcttgctttg gatcttcgcc tgtttttctt ccatccattt
     2941 cgcgagcttc gcatcggcct gttcctgcgt cagcgcgcct ttgaggacgc ccttgtgcag
     3001 gtgctggcga tacagcacgc ctttgtagga gagaatagcc cggcaggtgt ccgtgggctg
     3061 tgcgcctttc tggagccact cgaaggcggt gttgttgtcg agttcgattg ttgccgggtt
     3121 cgtgttagga ttataggtgc ctattttcgc aataaacttc ccatcccgtg gggcacgact
     3181 gtccgcaaca acgatgtgga aaaaagcaga agctttttta ccatggcgtt gaagtctgat
     3241 cttcgcaggc atttgttgtg atttgattgt tttaccgccc ggattttccg gaaacgggag
     3301 gcaaatgtag agaatttccg ggaaatgaca gcaaacaggg gcctgattac cgggagaccg
     3361 gcaacgcctt accgcgctcc cgggcggcaa aacaggagat ccaccccggc gcgatcctct
     3421 aagccggcac gcatcgcagg aaaaacttct cgttagattg gatgggatgg gaagtccccc
     3481 aggtgctgcc tggtggattt ttgataacct gccagggtgg attgttcttc gcctttgcat
     3541 cggcgttgaa gttccgcacg ggcacatcat tttcgtcgca gtatttgtac aggttgtaag
     3601 tcgtgcctgg ggtaagacca gaaacggtga tggtaagaat aaggggcatc ggttccggag
     3661 catctttcgt tccgctgatc tggggccatt cgtaattgag attcgtgtcg atgcgaaccg
     3721 gccgtgtttc cttgtccgga tcggccggac cggtaatggc aatgccgtag ttatggtaat
     3781 ttccggtccc aatgtatttc ggcaattcga gcagcgtata gatattgccg tccgggttat
     3841 ccgcgttttc cgtg
//
LOCUS       DASTSF010000097         2899 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_830458,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000097 DASTSF010000000
VERSION     DASTSF010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2899)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2899)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2899
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_830458"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1036
                     /locus_tag="VFU15_02945"
     CDS             <1..1036
                     /locus_tag="VFU15_02945"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEU4716757.1"
                     /translation="VQWGQCYNTPSPLYGGVLYSALPTADGDYICSGSFDYRMTLFKT
                     DANGNVLWCRITNTPGELFDSRATSDGGYIASGYQTSYSSGSSEAVLMKTNGAGTIQW
                     TDDYYLNTSTSSYDEGYAVVPDGSGYTALISDGYSGGRYLMHTDSAGVPAWAKNYGSV
                     SDLSFSLEKTTDNGFLLNGYSQDYMNVVVVKTDPSGNAACDDSTLVVDHDTVSYIMDS
                     VLTTVSATARNTPAVVFVNHTIVYQTICSANSESGVEDPGGGPPGLAVFPNPAQNSVS
                     VRMDMLHDNSVVISVTDLSGQVVSERKETNVSGTYERTLDLSSLAGGIYFIRVQVDDQ
                     PPAVRKVVHE"
     gene            1175..2416
                     /locus_tag="VFU15_02950"
     CDS             1175..2416
                     /locus_tag="VFU15_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008509466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU4716758.1"
                     /translation="MLFLTLLRESLLFALHALRVNRLRTILSLLGITIGIFAIIAVFT
                     AVDSMANKITSSVKSLGENVVFVQKWPWFTPGDYAWWKYMNRPVPKIFEADEVKKRST
                     KADAVVYTVNTRTTVNYGTSYVENATVVGTTFDYNKVKTFDLSSGRYFTQQEAETGRA
                     VCLIGSNIADALFHGQEALGPIKMYGRKVNVIGIFKSEGESMIGNTSDNEIVVPLNFA
                     RTLVDVQRDELDPYIMIKAKDNVAIPDLKDELTGIMRSLRRLKPVEEDNFALNEVSIL
                     SEPMEKMFGVVGNVGWIIGAFSILVGGFGIANIMFVSVRERTNQIGIQKSLGAKSWFI
                     LLQFLAESVILCLLGGVIGLVFDFILVLVANHFIDFGFILTASNISLGLTISVLIGII
                     SGFIPAYSAAQLDPVEAIRQN"
     gene            2450..2806
                     /locus_tag="VFU15_02955"
     CDS             2450..2806
                     /locus_tag="VFU15_02955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716759.1"
                     /translation="MSLAKTTKRPILISILCVAGFIFSAGQIILISSPSIRSIAGWYP
                     IVYGLITAARFISFVGVWNMKKWAAEMFLYVSLLKIIIQLLVHDSGVTSWADAGISLT
                     FAIIFLSYYKRMDRNL"
BASE COUNT          638 a          755 c          846 g          660 t
ORIGIN      
        1 ggtgcaatgg ggccaatgtt ataacacccc ttcgccgctt tacggaggtg ttctttattc
       61 ggctttgcca acggcggacg gtgactacat ctgcagcgga tcgttcgact acaggatgac
      121 gcttttcaaa accgacgcga acggtaatgt gctatggtgc aggataacga acacgccggg
      181 tgagcttttc gattcaaggg ccacaagcga cgggggctac atcgcttccg gctaccagac
      241 cagctactct tcgggttcat cggaagctgt gctgatgaag acgaacggcg caggaacgat
      301 ccagtggacg gatgactatt acctgaacac ttccacctcc tcgtatgacg aaggttacgc
      361 ggtcgttccc gacggaagcg gctacacggc gctgatcagc gacgggtaca gcggaggccg
      421 ttacctgatg catacggaca gcgcgggagt tccggcctgg gcgaaaaatt acggttccgt
      481 ttccgatctt tctttttcgc tggaaaaaac gacagataac ggtttcctgc tgaacgggta
      541 ttcgcaggac tacatgaatg tcgtcgtcgt gaaaacagac ccttccggca atgccgcctg
      601 cgatgacagc acgctggttg tcgaccacga caccgtttcg tacatcatgg attccgtgct
      661 taccaccgtc agcgccacgg caaggaacac gccggctgtc gtttttgtca accatacgat
      721 cgtttaccag accatctgtt ccgctaattc cgaaagcggc gtcgaagatc ccggcggtgg
      781 tccgcccggc ctggcggttt ttcccaaccc ggcgcagaat tccgtttcag tgcgtatgga
      841 catgctgcat gacaatagcg tcgtgatctc ggtgaccgac ctgtcggggc aggtcgtgag
      901 cgagcggaag gaaacgaatg tttcgggaac ttatgaacgg acgctcgatc tttcatcgct
      961 tgccggggga atttatttca tccgcgtgca ggtggatgat cagccgccgg cggtgcgtaa
     1021 ggtggtgcac gagtaatttc cttttgatgc gcagaagcag ctttcccggc cccgggtgag
     1081 caacgaggaa ccgcagggac gaggcgaaga cgggagcgaa ggttatttaa cgcacaggca
     1141 ggggcgcccc gaatgaaaac gagtaacttt gcccatgctt tttctgaccc ttctgcggga
     1201 aagtcttttg tttgcgctgc acgcactgcg tgtgaaccgc ctgcggacga tcctttccct
     1261 gctgggcatc accatcggta ttttcgcgat catcgccgtg ttcaccgccg tggattcgat
     1321 ggcgaacaag atcacgagca gcgtgaaatc gcttggcgag aacgtcgtgt tcgtgcagaa
     1381 atggccgtgg ttcacaccgg gcgattatgc gtggtggaaa tacatgaacc gtcccgttcc
     1441 caagatattc gaagcagatg aagtgaagaa acgcagcacg aaagcggacg cggtggtgta
     1501 cacggtgaat acgcggacga ccgtgaacta cggaacatcc tacgtggaga acgcgacagt
     1561 ggtgggaacg acgttcgatt acaacaaggt caagacattc gatctttcct cgggccgtta
     1621 ttttacgcag caggaagccg agacgggccg cgccgtgtgc ctgatcggga gcaatatcgc
     1681 cgatgcgctt ttccacgggc aggaggcact cgggccgatc aagatgtacg ggcggaaagt
     1741 gaacgtgatc ggcattttca aatcggaagg cgagagcatg atcgggaaca cgagcgacaa
     1801 cgagatcgtg gtgccgctga attttgcgcg cacgctggtg gatgtgcagc gggacgagct
     1861 cgatccgtac atcatgatca aagcgaaaga caacgtggcc attcccgacc tgaaagatga
     1921 gctcacaggc atcatgcgga gcctgcgccg gctgaaaccg gtggaggaag acaatttcgc
     1981 gctgaacgaa gtgagcattt tgtcggagcc gatggaaaaa atgttcggcg tggtgggcaa
     2041 cgtaggctgg atcatcggcg cgttctccat tcttgtgggc ggtttcggga tcgcgaacat
     2101 catgttcgtt tccgtgcgtg agcgcacgaa ccagatcggg atacagaaat cgctcggcgc
     2161 gaaaagctgg ttcattctcc tgcagttcct cgccgaatcg gtgatcctgt gcctgctggg
     2221 cggcgttatc gggctcgtgt tcgattttat cctggtgctg gtggccaacc atttcatcga
     2281 tttcgggttc atcctcaccg cgtcgaacat ttcgctgggt cttaccatca gcgtgctgat
     2341 cgggattatt tcgggattca ttcccgctta ttccgctgcg cagctcgacc cggtggaagc
     2401 gatacggcag aactgatccc gggcgcgacg gaaaagaaaa aagagacgca tgtcactggc
     2461 aaaaacgacc aaacggccca tcctgatctc catcctctgc gtggccggtt ttatcttttc
     2521 cgcaggacag atcattctca tttcctcgcc ttcgatccgt tcgatcgccg ggtggtaccc
     2581 gatcgtttac gggctgatca cagccgcgcg ttttatttca ttcgtcggcg tatggaacat
     2641 gaaaaaatgg gcggcggaaa tgttcctgta tgtttcgctg ctgaagatca tcatccagct
     2701 gctcgtgcac gattcgggcg tgacaagctg ggccgacgca ggaatttcgc ttacgttcgc
     2761 gatcattttt ctcagctatt acaagcggat ggacaggaat ctgtaaattc taattccgtt
     2821 ttattttccg gttgaatgtg tgttgcacat ttccttcctg gtcgcagaaa aagaaaggga
     2881 acgtctcgac ttgcgcccg
//
LOCUS       DASTSF010000098        11019 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1502175,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000098 DASTSF010000000
VERSION     DASTSF010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 11019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11019
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1502175"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(333..788)
                     /locus_tag="VFU15_02960"
     CDS             complement(333..788)
                     /locus_tag="VFU15_02960"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU4716760.1"
                     /translation="MNTSIHKNSKNRKFLPQEFTVFIVDDDAFYLSALAFRLMKTIHD
                     HNVKIFCYSSGEECVQNLGLHPSLVILDYYLSSGQQAAMSGMDVMRRIKSVYPHVPVI
                     MLSGQHSMSVALNTLREGAYTYVIKDKLAPYSIEKIISRFVSGRSAAVH"
     gene            complement(811..1512)
                     /locus_tag="VFU15_02965"
     CDS             complement(811..1512)
                     /locus_tag="VFU15_02965"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU4716761.1"
                     /translation="METTMMENTLPAVKEVRKKDTEVRIAIVEDDPMFRHAMECYLNK
                     LPGNRVYSFDSGESCFRHYHLIDPEILILDYRLNENSEAGGMDGLDILHEVKSVHPAI
                     EVIFVSGQESFEVATAAIKGGASDYIIKDDKALKNMLDDVARISTDIRSRRERSRILT
                     WIFVIAAFTLGVLLFASLTDYTGATGPLKILLVAAAAAFVVVALFPERKKKRKQGLST
                     GFFHSRKERPGMWVD"
     gene            1954..5616
                     /locus_tag="VFU15_02970"
     CDS             1954..5616
                     /locus_tag="VFU15_02970"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEU4716762.1"
                     /translation="MKNFILLRWLNNVSISRKLYFTVGIMALLIALELATLLFAVNTL
                     SSVRAYVGGEGLWSKAQKDAIYHLEKYGHSRNETDYADFLRFMRVPLGDHKARMEMQK
                     KEPDINVVRLGFIEGRNNPQDVDGMYHLFRRFYRISYIEKAIRIWVQADSASAPLLPL
                     GEQLHKEITSVSPSQEKIDAILQRITPINLGLTRLEDDFSYTLGEGSRWLEQLILKIL
                     FCIALTVEISGLLLTFSVSKGIHRGLKEIIRASETVATGNLQVHAQKFSQDEIGQLAD
                     SFNRMTDELRQNIHARKQSNVLLRESEERFRLMMKNIKDYAIFMTDVNGYVISWNEAA
                     QRISGYYGEDIIGKHISVFYSDEQVHAGEPMKNLENALKNGDYRVEGWRTRKDGTRFY
                     ADVLYTALYDDDGHLKGYAKIIRDITEQKKAEISMRQNEALLIMAQHVAHLGSWEWDL
                     ETGSLVWSEEMYRIYGLTQNDRIDPATVVSMTHPADREHVKAVTDETKKLCRPFDFNY
                     RIIRKDNVVRILHARGEVVIQNNGSVKKMIGTAQDITETILEHEMEKLATAATKSYNA
                     VVIADSTGNIEWVNEGFSRLSGYSLEEVKNTHGEILRRGDPTGLSRQMQFYEQILRDK
                     KPVTYEGKNFSRSGKEYWVITTLTPVLGKDGNVERIIAIDSDITERKQIEEDLILANR
                     IAEHSLKKGSRALSELMKAKKELEESLHIKEQFLAKMSHEIRTPMNAIVGLTDLLIEG
                     ENLPEQKECLEAVKLSADNLLSIINDILDFSKLESGKVTFEKQPFRVNDVISGVLLTL
                     DYAARKKNISLKGIADMQEIPEYVNGDALRLRQILLNLAGNSIKFTEKGNVTVKAELA
                     SRDEENCVLRFTVTDTGIGIAEDKLPAIFESFTQASNETTRKYGGSGLGLTIVRQLTD
                     QQGGSVEVKSKVGKGSVFTVTLPYGISRPEDIAQADAAELQSTEIPEKVKVLLAEDNE
                     MNQMLTRKVFEKWGFALDIAENGKIAVEKLDVEEYDLVLMDIQMPEMDGYEATRFIRS
                     NLPSPKSSIPIIAMTAHAIVGEKEKCIGCGMDDYISKPFDRKMLYEKICRLLHREPVP
                     GEDASPEKDLPSAGMAGEHVTDLTYLRGIAEGNNVFMRKMINAFLGQTPGMLSQMKQY
                     LGSSQWKELRGVAHKMKPSLDFIGLSSIKETVREIEKNAMDETNLETLPDLIGHVTVV
                     CTKAMEELEKELQELE"
     gene            complement(5651..6562)
                     /locus_tag="VFU15_02975"
     CDS             complement(5651..6562)
                     /locus_tag="VFU15_02975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716763.1"
                     /translation="MFLRAFRSVEKFLKGLPVIRDIPGFNSLPEAITPHNLRFYKLNN
                     LVQTLGMFVHASWILLFAVLHVYTLAYINVLSVAIYIFNIVINRRGYHFTASSIMVTE
                     IAVHQIIAVKFFGLDAGFQYYLIVITLFPFLMPKGKWTLKGILLAGCLTTFILLDYFL
                     KDAQPWETPGSGTLGFIRISNIAMSFISLCISGAYFNLAMHETEADLAKKTSELIEAE
                     KKATLGNVATEMAHEIQNPLNFVNNFSEMNEELLLELKNEMERSGSNEEAIAILNDLL
                     ENSKRIKTNGQRVSKIVSLLQDRVNKL"
     gene            complement(6631..7521)
                     /locus_tag="VFU15_02980"
     CDS             complement(6631..7521)
                     /locus_tag="VFU15_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HEU4716764.1"
                     /translation="MNATLFALPGNETAAKLISGRLGCETGQCILRQFPDGETYLRLE
                     SDVRGKTIILLCTLDRPDAKIIPLYFFAGTLRKAGARKILLVAPYLAYMRQDKQFHPG
                     ESVTSEHFAALLSSFTDGLITVDPHLHRRKSLNEIYTVPSVAVHAAEHIAEWIKQNVK
                     QPVLVGPDGESGQWVSEVAKLAGAPFTVLTKERTGDAEVKISVPHIAQFRNTHTPVLV
                     DDIISTGRTMTGTIAHLREEKMKPTVCIGIHAVFSGDAYSDLKRAGVEKVVTCNTIPH
                     ESNAINVLDSVAGSIGKIKL"
     gene            complement(7527..9089)
                     /locus_tag="VFU15_02985"
     CDS             complement(7527..9089)
                     /locus_tag="VFU15_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015752952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase family protein"
                     /protein_id="HEU4716765.1"
                     /translation="MKSSNKTQKHHRGKEADDEPVQEKKHGLKLKRLGIDTHKEHVAY
                     MRADCHVCVSEGFEALSRIRITNTEHSIVANLNTVNSDLLSEGEIALSEMAFLALHAE
                     EGAYVSVSHLGPLHSMSYVRAKIFGKQIPPEGIDEIVSDVVKGNYSNVHLASFITAFA
                     GDHMSAGEVISLTKAMIACGNRITWGKNMIVDKHCIGGLPGNRTTPIVVPIIAAAGLT
                     MPKTSSRAITSPAGTADTMEVMTPVNLGIEKIKEVVEKEGACIAWGGIANLSPADDIL
                     IRVERALDLDSEGQMIASVLSKKVAAGSTHVVIDMPVGETAKLRSIESANHLMVMMEE
                     VAHAIGLKIKVVITDGTQPVGRGIGPVQEAIDVLSVLRNEEDAPPDLRQRALLIAGEL
                     LELSGKAEQGEGNKMASALLTSGKAYEKFLAICEAQGGFTEPVQAKYTHQVAAEKTGT
                     VVSIDNRRLAQIAKLAGAPDHRAAGIKFLSPAGKKVTKGEALFIIYTEAEGQLEYALQ
                     YYESEKNIITIN"
     gene            complement(9086..9964)
                     /locus_tag="VFU15_02990"
     CDS             complement(9086..9964)
                     /locus_tag="VFU15_02990"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HEU4716766.1"
                     /translation="MANRKKYVINASGENIPFSPEKLAQSLRHSGADEKTIAGITREI
                     SRHIYDGVSTKKIYRQAYAMLRKRSPHFGGRYKLKEALLELGPSGFPFEKFVARVLHH
                     RGYETKLNVIMQGSCVRHEVDVLAAKDNNEIMIECKYHNQRGLKSDVKIPLYVHSRFR
                     DIQRYREQRTEKSLVAFTGWLVTNTRFTGDAYQYGTCAGLHLVSWDQPENESLKEMID
                     MTGLHPLTCMSSLSKSEKIALLHEGVVLCNDLENSRDLLYKMDIPPARVERIIREAGR
                     ITQNAMQEPMQKAKQV"
     gene            complement(9987..10895)
                     /locus_tag="VFU15_02995"
     CDS             complement(9987..10895)
                     /locus_tag="VFU15_02995"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011022404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEU4716767.1"
                     /translation="MDTLESLRRKIGSAGELESVVRTMKAMAASGIGQYERAVAALRD
                     YYRTVELGMIAYFRQEIHATAAGEKKKEKQKRVFAIVFGSDQGLVGQFNETMAEFVSA
                     KLQSSGKITEIWTIGERVNDRLTDAGFPPVKTFPVPNSLGGITSLVGKILAEMEEVTG
                     NESNEVFIFHNKPRERAGYEPSGNRLFPPDEKWKNEAEEKKWPANKQPQVVGGQEHTL
                     AALVREYLFVSVYRACAESLACENAGRLEAMQRAEKNIDELMEELNHKYHRLRQSSID
                     EELFDVVSGFEALKKNRTANSKRGYY"
BASE COUNT         2492 a         3059 c         2906 g         2562 t
ORIGIN      
        1 tgcttctcaa cgagatcccg agaaattcat aagcgtgccc gggaacggaa aacggcagaa
       61 gaaatgacgg acatgatgat cattcttctg cctgagatcc tgatggtgca atgggaaaac
      121 agcacggagg cacggagcgg atgtacagat catctgacct gtttcgtccc tgcgttctgc
      181 cttgcaacgt tccggtgaca acgaacttat tcaacttctg ttgcgaaaat aattcacgcg
      241 tcatttcctc ccggcgcgtt ttcgacgccc ggctgtattt cacccgcatc ctgctcttcc
      301 gggacgacat cccgggaaaa aacggccaca catcagtgca cggcggcgga tctcccggac
      361 acgaaccgcg aaatgatctt ttcgatcgag tacggtgcga gcttgtcttt gatcacgtaa
      421 gtgtacgcgc cttcgcgcag cgtattcagc gcaaccgaca tgctgtgctg cccggaaagc
      481 atgatcaccg gcacgtgcgg gtaaacgctc ttgatccttc tcatcacgtc cattccgctc
      541 atggcggcct gctgtccaga actgagataa tagtccagga tcaccagcga aggatgcagg
      601 ccgagattct gcacgcattc ctcgccggag ctgtagcaga aaattttgac gttatggtcg
      661 tggatcgttt tcatcaggcg gaacgccaat gcagaaagat agaaagcatc gtcgtccacg
      721 atgaacacgg taaattcctg cgggaggaat ttcctgtttt tgctgttctt atggatggat
      781 gtgttcatga ctttcgttat tgcggttcag tcagtcaacc cacattcccg gccgttcttt
      841 ccgtgaatgg aaaaacccgg tggaaagacc ctgctttctt tttttcttcc gctccgggaa
      901 aagcgccacc actacgaaag cggcggctgc agcgacgagc aggatcttca gcgggccggt
      961 ggcgcccgta taatcagtga gcgatgcaaa aagcagcacg cccagcgtga aagcagcgat
     1021 gacgaagatc cacgtgagga tgcgggaacg ttcgcggcgg gaacggatgt ccgtcgatat
     1081 gcgcgcgaca tcgtccagca tgtttttcag cgccttgtcg tccttgatga tatagtcgga
     1141 cgcaccgccc ttgatcgccg cggtcgccac ttcgaagctt tcctgcccgg aaacgaagat
     1201 cacctcgata gcaggatgca cgcttttcac ttcgtggaga atgtccagtc cgtccatgcc
     1261 gcccgcttct gaattttcat tcagccggta atcgagaata agaatctcgg gatcgatcag
     1321 gtggtaatgc ctgaaacagg attcgcccga atcgaacgaa tacacccggt tgccgggcag
     1381 cttgttcagg taacattcca tcgcatgcct gaacatcgga tcgtcttcca cgatcgcaat
     1441 ccgcacttca gtgtctttct tccgcacctc ttttacagca ggcagggtgt tttccatcat
     1501 tgttgtttcc atgacacagc ggttttgatt tgcataccta atttacgaag cagcgaagcg
     1561 ggccggactg caatttcgtc gcatggacga attccggcag acggttggaa gattgcttta
     1621 catggttgga aaaaaacgca ggatttaccg caaaaaacag cgcagaccgc taaccttttt
     1681 ttcgccgggg agtataatat aatccagaat tgcggtacgc atggaacctg cctgctgaac
     1741 ggctcccgga gggatgtccg tgcagtttta ttccgggcag gcaataaata cgcttcactt
     1801 gttttttctg tcccctgtga accgggaaaa agaaaaaagc atgagaacag gcggtctttc
     1861 attccggcag tatcttttcc accgcattgt ttccctgcgt acttctcttt ccgcattttt
     1921 tgcggcggag aaacgaactg aacctgtcat cctatgaaaa acttcatact cctcaggtgg
     1981 ctcaacaacg tttccatttc gcggaaactg tatttcaccg tgggcatcat ggccctgctg
     2041 atcgcgctgg agctggcgac gctgctgttc gccgtcaata cgctttcatc ggtgcgcgct
     2101 tacgtgggcg gggaaggtct ctggtcgaaa gcgcagaagg atgccatcta tcatcttgaa
     2161 aaatacggcc actcgcgcaa tgagacggac tacgcggatt ttctccgttt catgcgcgtt
     2221 ccgctcggcg atcataaagc gcggatggag atgcagaaaa aagaaccgga catcaacgtt
     2281 gtgcgccttg gtttcatcga aggccgcaac aacccgcagg acgtggacgg aatgtaccac
     2341 ctgttccgcc gcttttaccg catctcctac attgaaaaag cgatccggat atgggtgcag
     2401 gccgatagcg ccagcgcgcc cctgctcccg ctcggcgagc agctgcacaa agagatcacc
     2461 tccgtatcgc cttcgcagga aaagatcgac gcgatactgc agcgcattac gcccatcaac
     2521 ctgggcctga cgcggctgga agatgatttt tcctatacgc tcggcgaagg ttcgcgatgg
     2581 ctcgaacagc tcatcctgaa aattcttttc tgcattgcgc tcacggtcga gatcagcggg
     2641 ctgctgctta cgttttccgt cagcaaggga attcaccgcg ggctcaaaga gatcatccgc
     2701 gcttcggaaa cagtggcgac aggaaacctg caggtgcacg cgcagaaatt ctcgcaggac
     2761 gagatcggcc agctcgcgga ttcattcaat cgcatgacgg acgagcttcg ccagaacatt
     2821 catgcgcgca aacagtcgaa cgtgttgctg cgtgaaagcg aggaacgctt ccggctgatg
     2881 atgaaaaaca taaaagacta cgccatcttc atgaccgacg tgaacggcta tgtcatcagc
     2941 tggaacgagg ccgcgcagcg gatcagcggg tattacggcg aagatatcat cggaaaacat
     3001 atttccgttt tttattccga cgagcaggtg cacgccggcg agcccatgaa aaacctggag
     3061 aacgcattga agaacggcga ttaccgcgtt gaaggctggc gtacccggaa agacggaacc
     3121 cgcttttacg ccgatgttct ttacactgcg ctttacgacg acgacggtca cctgaagggt
     3181 tacgccaaga tcatccgcga tattacggaa cagaaaaaag ccgagatctc catgcggcag
     3241 aacgaagcat tgctgatcat ggcgcagcat gtcgcacacc tcggcagctg ggaatgggac
     3301 cttgaaacag gatcgctcgt ctggtcggaa gagatgtaca ggatatacgg gctcacacag
     3361 aacgacagga tcgatccggc aaccgtcgtc agcatgacgc accctgccga ccgggaacac
     3421 gtgaaagcgg ttacggacga aacgaaaaag ctgtgcaggc ccttcgattt caattaccgc
     3481 atcatccgga aggataatgt tgtccgcatt ttgcatgccc gcggcgaggt agtaatacaa
     3541 aacaacggca gcgtgaaaaa gatgatcggc accgcgcagg acattacgga aacgatactc
     3601 gagcacgaga tggaaaagct cgccaccgcg gcaacgaaat cctacaacgc ggtcgtgatc
     3661 gccgacagca ccggcaacat cgaatgggtg aacgaaggtt tttcccgcct gagcggctat
     3721 tcgctcgaag aagtgaaaaa cacgcacggc gagatcctgc ggcgcggaga cccgaccggc
     3781 ctttcccggc agatgcagtt ctatgagcag atcctccgcg ataaaaaacc cgtcacgtac
     3841 gaaggaaaaa acttttcgcg cagcggaaaa gaatactggg tcatcaccac gctcacgccc
     3901 gtgctcggca aggacggcaa cgtggagcgc atcatcgcga tcgattcgga tatcaccgaa
     3961 cgcaagcaga tcgaggaaga tctcattctc gccaaccgca tcgccgaaca ttccctgaaa
     4021 aaaggaagcc gcgcgctttc cgagctgatg aaggcgaaaa aagagctgga agaatcgctc
     4081 cacataaaag agcagttcct ggcaaagatg agccacgaga tccgcacgcc gatgaatgcg
     4141 atcgtcgggc tgacggacct gctgatcgaa ggagagaacc tgcccgagca gaaagaatgc
     4201 cttgaagccg tcaagctttc cgccgacaat cttctttcga tcatcaatga catcctcgat
     4261 ttttcaaagc tcgaatccgg gaaggtcacg ttcgagaaac agccgttccg cgtgaatgac
     4321 gtcatcagcg gcgtgctgct cacgctggat tacgcggcgc gcaagaaaaa tatttcgctc
     4381 aagggaatcg ccgacatgca ggaaattccc gagtacgtga acggggatgc gttgcgcctg
     4441 cgccagatcc tgctcaacct tgccggcaat tcgataaaat tcacggagaa ggggaacgta
     4501 accgttaaag cggaactggc ttcgcgcgat gaggaaaact gtgtgctgag attcacggtg
     4561 accgataccg gcatcggcat tgcggaagac aagctgccgg cgatcttcga aagcttcacg
     4621 caggccagca acgaaacgac gcggaaatac ggaggctccg gcctcgggct gacgatcgtc
     4681 cgccagctta ccgaccagca gggcggttcc gtggaagtaa aaagcaaagt gggcaaaggt
     4741 tccgttttca ccgtgacgct tccgtacgga ataagcaggc ccgaagacat tgcgcaggcc
     4801 gacgcggccg agctgcaatc gacagagatc ccggaaaaag tgaaagtgct cctggccgaa
     4861 gacaacgaga tgaaccagat gctcacgcga aaagtattcg agaaatgggg ctttgccctc
     4921 gacatcgcgg aaaacgggaa gatcgcggtg gaaaagctgg atgtggaaga atacgatctc
     4981 gtcctgatgg atatacagat gccggaaatg gatggttacg aagcgacgcg tttcatccgc
     5041 agcaacctgc cttccccgaa atcttccatt cccatcatcg cgatgacagc gcatgcgatc
     5101 gtcggcgaaa aggaaaagtg catcggctgc ggaatggacg attacatttc aaaaccgttc
     5161 gaccggaaaa tgctttacga aaagatctgc cgcctgcttc accgcgaacc tgttccgggg
     5221 gaagacgcat cgccggaaaa agatcttcct tccgctggta tggccgggga gcacgtcacc
     5281 gacctgacgt acctgcgcgg cattgcggaa gggaataatg tgttcatgcg gaaaatgatc
     5341 aacgcgttcc tcggccagac accgggaatg ctcagccaga tgaaacaata cctcggcagc
     5401 agccagtgga aagagctccg gggcgtcgcg cacaaaatga aaccctcgct ggatttcatc
     5461 gggctcagct cgatcaagga aacggtccgc gagatcgaaa agaacgcgat ggatgaaacg
     5521 aacctcgaaa cgcttcccga tctgatcgga cacgtcaccg tcgtgtgcac gaaagccatg
     5581 gaagagctgg agaaagagct gcaggagctg gaatagaaaa ttgcgaaggc accgcagcga
     5641 gcagcgacga tcagagcttg ttcacacggt cctgcagcag gctgacgatc ttcgaaacgc
     5701 gctgcccgtt tgttttgatg cgcttgctgt tctccaggag gtcgttcagg atcgcgatcg
     5761 cttcttcgtt gcttcccgat ctttccatct cgtttttcag ctccagcaaa agctcttcat
     5821 tcatctcgga aaaattatta acgaagttca gcgggttctg tatctcgtgc gccatctcgg
     5881 tcgccacatt gcccagcgtc gcttttttct ccgcttcgat cagctcgctg gtctttttcg
     5941 cgagatccgc ttcggtttcg tgcatagcga gattaaaata cgctccgctg atgcagagtg
     6001 aaatgaacga catggcgatg ttggaaatgc ggatgaagcc gagcgtgccc gaaccgggcg
     6061 tttcccaggg ctgcgcatct ttcaggaaat aatcgaggag gatgaaggtc gtcaggcagc
     6121 cggcgagcag aattcccttc agcgtccatt ttcccttcgg catcaggaac gggaaaagag
     6181 tgatcacgat aaggtaatac tggaaccccg cgtcgagccc gaagaacttc acggcgataa
     6241 tttgatgaac ggcgatctcc gtgaccatga tggatgaggc ggtgaaatga tacccgcgcc
     6301 ggttgatgac gatgttgaaa atgtaaatgg caacgctgag aacgttgatg taggccagcg
     6361 tgtaaacatg cagaacagcg aaaagaagta tccacgatgc atgcacgaac atgccgagcg
     6421 tctgcacgag gttgttgagc ttgtagaaac gcaggttgtg cggcgtaatg gcttccggaa
     6481 gcgagttgaa cccgggaatg tcgcggatca ccggcaaccc tttcaggaat ttttcaacgc
     6541 tacggaatgc gcggaggaac atcaggctgg aaaaagctga tgcaatatta cggggattgc
     6601 gcccgaaaaa caatgcgccg caaggatggt ttacagcttt atttttccaa tggaaccggc
     6661 aacactatcg agaacattta tcgcattgct ttcatgcggg atcgtgttgc acgtgacgac
     6721 cttttccaca cccgcacgtt tcaggtcgct gtacgcatcg cctgaaaaaa cagcatgaat
     6781 gccgatgcag acggtcggtt tcattttttc ttcccgcagg tgcgcgatcg ttcctgtcat
     6841 cgtgcgtccc gtggaaataa tgtcgtcaac gagcacgggc gtgtgcgtgt tcctgaactg
     6901 cgcgatgtgc gggactgaaa ttttcacttc ggcatcaccg gtcctttctt tcgtaagcac
     6961 ggtgaacggt gcgcccgcga gcttcgccac ttcactcacc cactgcccgc tttccccgtc
     7021 cggaccgaca agcaccggct gcttcacgtt ttgttttatc cattccgcaa tatgttccgc
     7081 agcatgaact gccacagacg gaactgtata gatctcgttc agcgacttcc gcctgtgcag
     7141 gtgcggatcg acggtgatca gcccgtcggt gaatgaagaa agcagcgccg caaaatgttc
     7201 cgacgtgact gattcaccgg gatggaattg cttgtcctgc cgcatgtaag ccaggtaggg
     7261 tgcgaccagc aggatctttc ttgcgccggc ttttctcagc gttcccgcga aaaaatagag
     7321 cgggatgatc ttcgcatcgg gacggtcgag cgtgcagaga agaatgatcg tctttccgcg
     7381 cacatccgac tccaggcgca ggtaggtttc tccgtcagga aactgacgca ggatgcattg
     7441 ccccgtttca catcccagtc ttccggaaat cagcttcgca gcggtttcat tgcccggcaa
     7501 agcgaacagc gttgcattca tacgcgtcag tttatcgtga tgatgttctt ttcggattca
     7561 tagtattgaa gcgcatattc cagctgtcct tccgcttccg tgtaaatgat aaaaagcgct
     7621 tcgcccttcg tcactttctt tcctgccggc gacaggaatt ttattcccgc ggcgcgatgg
     7681 tccggcgcac cggcaagctt cgcgatctgc gccagcctgc ggttgtcgat gctcaccacg
     7741 gtgcctgttt tttcagcagc gacctggtgc gtgtattttg cctgcaccgg ttccgtgaat
     7801 ccgccctgcg cttcacagat ggcgaggaac ttttcgtacg cttttcccga cgtgagcagc
     7861 gcggacgcca ttttatttcc ttccccctgc tccgctttcc cggatagctc gagcagctca
     7921 cctgcaatga gcagcgctct ttgcctcagg tccggcggcg cgtcctcttc attgcgcagc
     7981 accgacaata catcgatcgc ttcctgcacg gggcctatgc ctcttcccac cggctgcgtt
     8041 ccgtcggtga tcaccacttt tattttcagc ccgatcgcat gcgccacttc ctccatcatc
     8101 accatcaggt ggttcgcgct ttcaatgctg cgcagcttcg ctgtttcgcc caccggcata
     8161 tcgatcacga cgtgcgtgga gcccgccgcc acttttttcg acagcacgga agcgatcatc
     8221 tgtccctcgc tgtcaagatc gagcgcacgt tccacgcgga tgaggatgtc atccgcagga
     8281 ctgaggttcg caattccgcc ccacgcaatg caggcgcctt ccttctccac gacctctttt
     8341 attttttcga ttcccaggtt caccggcgtc atgacctcca tcgtgtccgc tgttccggca
     8401 ggcgaggtga tcgcgcggga agatgttttc ggcatggtga gcccggccgc ggcaatgatc
     8461 ggcaccacga tcggcgtcgt gcggttgccg ggtaacccgc cgatgcaatg tttgtcgacg
     8521 atcatatttt ttccccaggt gatcctgttg ccgcaggcga tcattgcttt tgtcagtgaa
     8581 atgacttcac cggcgctcat gtggtcgcct gcaaacgccg tgatgaacga cgcgaggtga
     8641 acgttggagt agtttccttt caccacgtcg ctcacgatct cgtctattcc ttccggcggg
     8701 atctgctttc cgaagatctt tgcccgcacg tagctcatgg aatgaagcgg cccgaggtgg
     8761 gatacggaaa cgtatgcgcc ctcttccgca tgtagcgcga ggaaagccat ttccgaaagc
     8821 gcgatctccc cttcgctgag caggtccgaa ttaaccgtgt tcaggttggc cacgatggaa
     8881 tgttccgtgt tggtgatgcg tatgcgcgag agcgcttcaa atccttccga cacgcagaca
     8941 tgacagtccg cacgcatgta cgcgacgtgc tccttatgcg tatctatgcc gagacgtttc
     9001 agcttcagtc cgtgcttttt ttcctgcacc ggttcgtcgt ctgcttcttt cccgcggtga
     9061 tgcttttgtg ttttgttgct gctcttcata cctgttttgc tttctgcatt ggttcctgca
     9121 ttgcattttg agtgatcctg ccggcttcgc ggatgatgcg ttcaacacgt gctggcggaa
     9181 tgtccatttt ataaagaaga tcacggctgt tctccaggtc gttgcacagc acgacacctt
     9241 cgtgaagaag ggcgatcttt tcactttttg acagcgagct catgcaggtc agcggatgaa
     9301 ggccggtcat gtcgatcatt tctttcaggc tttcgttttc cggctgatcc cagctgacga
     9361 gatgaagtcc cgcacacgtt ccgtattgat aggcgtcacc ggtgaagcgc gtattcgtga
     9421 ccagccaccc ggtaaatgcg accaaacttt tttccgtccg ctgttcccgg taacgctgta
     9481 tatcacggaa acgtgaatgc acatacagcg ggatcttcac atcggatttc agtccgcgct
     9541 ggttgtggta tttgcattcg atcatgatct cgttgttatc cttcgcagcc agcacatcca
     9601 cttcatgcct gacgcagctg ccctgcatga tcacgttcag ctttgtttca taaccccggt
     9661 gatgcagcac gcgtgcgaca aatttttcaa acgggaatcc tgaaggtcca agctcgagga
     9721 gcgcttcttt cagcttgtac cttccgccga aatgcggcga tcttttccgc agcatcgcat
     9781 acgcctgccg gtaaattttc tttgtcgaaa caccatcgta aatgtgcctg cttatttcac
     9841 gggtgatgcc agcaatggtc ttttcgtccg cacccgaatg ccggagcgat tgcgccagct
     9901 tttcaggcga gaagggaatg ttttcccctg atgcattgat cacgtatttt ttccggttgg
     9961 ccatacctgt ttttcgttac gaatgttcaa taataaccgc gcttactatt cgcggtcctg
    10021 ttttttttca gcgcttcgaa gccggaaacg acgtcgaaca gctcttcatc gatcgagctt
    10081 tggcgcaacc tgtggtactt atggttcagc tcttccatca gctcatcgat gttcttttcc
    10141 gcacgctgca tggcctccag ccggcctgca ttctcgcagg caagcgattc ggcgcaggcg
    10201 cggtaaaccg atacgaacag gtattcgcgc acaagcgccg cgagtgtatg ctcctgcccg
    10261 cccacaacct gaggctgttt gttcgccggc cacttttttt cttctgcctc gttcttccat
    10321 ttttcatccg ggggaaacaa ccggttaccg gaaggttcat acccggcgcg ttcccgcggc
    10381 ttgttgtgaa aaataaagac ttcattgctt tcatttcccg tcacctcttc catttccgca
    10441 agaatttttc caaccagcga tgtaatgccg cccagggaat tcggcaccgg aaatgttttg
    10501 accggcggga aaccggcatc ggtcaaacga tcgttcacgc gttccccgat cgtccagatc
    10561 tccgttattt ttccggatga ttgaagcttc gcggaaacga attccgccat ggtttcgttg
    10621 aattgcccca caagtccctg gtctgatccg aaaacgatcg caaatacccg cttttgtttt
    10681 tccttctttt tttcaccggc agcggttgcg tgaatttcct gcctgaagta cgcgatcatc
    10741 ccgagctcca ccgtccggta ataatcgcga agcgctgcca cagctctttc atactgcccg
    10801 atgccggaag cggccatggc tttcatggtc cgcacaaccg attccagctc gcccgcgctg
    10861 ccgatctttc tcctcaggct ttcgagtgtg tccatcgtta ttcgctttta ggtgctgttc
    10921 ctttttcttc ttcgccggca atcagcggct caagcgcttt ccgcgccgcc tgcaggatga
    10981 ccggtgtatc ggcttccgcc agcgcttccc cggcaaaga
//
LOCUS       DASTSF010000099         4074 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_382148,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000099 DASTSF010000000
VERSION     DASTSF010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4074
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_382148"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="VFU15_03000"
     CDS             <1..234
                     /locus_tag="VFU15_03000"
                     /inference="COORDINATES: protein motif:HMM:NF039201.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEU4716768.1"
                     /translation="PSMGKATLRLQVSHADEFEVYVRDVSGRQIIRKISLGRLAEGER
                     DVDLDLSAMENGFYLIEVRSATGSSWQKLILQH"
     gene            389..2848
                     /locus_tag="VFU15_03005"
     CDS             389..2848
                     /locus_tag="VFU15_03005"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HEU4716769.1"
                     /translation="MNDYLIYLVPALGIVGLIVMAIKAAWVSKQETGDANMVELSGYI
                     AKGAMAFLKAEWRILGFYVVIAAILLGWSGTLVDHSSPVIAISFVIGAVFSALAGYIG
                     MRIATRANVRTTQAARTSLAKALKVSFSGGTVMGLGVAGLAVMGLGGLFIVFYQLFVP
                     AGEAITGDGMKKAIEVLAGFSLGAESIALFARVGGGIYTKAADVGADLVGKVEAGIPE
                     DDVRNPATIADNVGDNVGDVAGMGADLFGSYVATILATMVLGQELKADDQFHGLSPIL
                     LPMVIAGMGLVFSIIGTFFVRIKDEKSSVQAALNMGNWSSIILTAIASYFLVNWMLPD
                     VMHWTTASRGADVTKGGVIGAIITGLIVGTIMSLVTEYYTAMGKRPVKSIVQKSATGH
                     ATNIIGGLSVGMESTVIPILVLAAGIIVSYSFASLYGVAIAAAGMMATTAMQLAIDAF
                     GPIADNAGGIAEMSQLPPEVRERTDNLDAVGNTTAATGKGFAIASAALTSLALFAAFV
                     GIAGISSIDIYKAPVLAALFIGGMIPFIFSSLAIAAVGRAAMDMVNEVRRQFREIPGI
                     MEYKAKPEYEKCVAISTKASIREMIAPGAIALLVPVLVGFGFKGVFPGTSSPEILGGL
                     LAGVTVSGVLMGMFQNNAGGAWDNAKKSFEKGVEINGETFYKKSDPHKASVTGDTVGD
                     PFKDTSGPSMNILIKLMSIVSLVIAPHIAVPKTDAACSMDGDHNKSCCMMQNTKCDMD
                     KCMHMSKEQCAHYCDSMGCSAEEKEMCMEHAGMQCCKDSASCDHDKCGSSSCMPGCTN
                     HCSSKEECEKNCGDKCAAGHK"
     gene            2937..3485
                     /locus_tag="VFU15_03010"
     CDS             2937..3485
                     /locus_tag="VFU15_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008463110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU4716770.1"
                     /translation="MPEFSFSPFPVLETERLILRRVEPADVAEMFVLRSDRQLMKFIP
                     RPVAQSPDDALALIHSMAEGIAKNEMINWGMVLKSSGKVIGTVGFFRMKPEHFRAEIG
                     YMLHGDFHGTGIMQEALVKAIAFGFQSMKLHSIEAVVDPANIASIRLLERNGFVREAY
                     FRENEFFDGKFLDSAVYSLLNR"
     gene            complement(3559..>4074)
                     /locus_tag="VFU15_03015"
     CDS             complement(3559..>4074)
                     /locus_tag="VFU15_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014203280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEU4716771.1"
                     /translation="DENPYLSDFYEDMQRWSFNLQIFFLNSRFAQVQEIRQSKRNVIQ
                     DRTIYEDAHIFAPNLHSMGLMTTRDFENYFSLFRLMESLIAPPDLIIYLRASVPTLVS
                     QIQKRGRDYENAIRIDYLKRLNERYEAWISTYEAGKLLIVDVDENKFSDNPEHLGVII
                     NKIDAEINGLF"
BASE COUNT          943 a         1030 c         1107 g          994 t
ORIGIN      
        1 ccgtcaatgg gaaaagctac gctgcgcctg caggtaagtc atgcagatga gttcgaagta
       61 tacgtgcgcg atgtttcagg acgccagatc atcaggaaaa tttctcttgg cagacttgct
      121 gaaggcgaaa gagatgttga cctcgatctt tctgcaatgg aaaatggttt ttacctcatc
      181 gaagtccgtt cagccaccgg ctcatcctgg cagaagctca ttttgcagca ctgaccggat
      241 ttacacgctg tcccccggcc caggttttct taacggaaag aactcatttt cacctattcc
      301 gggttacatt ttttcttcgt aagtttgcgc cccgataacg gggaagggcg cttataatag
      361 cctgatccta aatacataac aataaaatat gaacgattat ctgatctacc tcgtccctgc
      421 tttaggtatc gtgggactaa tagtaatggc aatcaaagct gcctgggttt ccaaacagga
      481 aaccggagac gccaacatgg ttgaattgtc cggctacatt gccaaaggag caatggcttt
      541 cctgaaagcc gaatggagaa ttctcgggtt ctatgtcgtt attgccgcga ttcttctcgg
      601 gtggtcaggc acactggttg atcattccag tcctgtcatc gccatttcat tcgtgatagg
      661 tgccgtattc tctgcacttg cagggtatat cggtatgcgt atcgcgacgc gcgccaacgt
      721 ccgcacaacg caggctgcgc gcacaagcct cgccaaagcg ctgaaagttt cattcagcgg
      781 cggaacggtt atgggactcg gcgtagccgg tcttgccgtg atgggactgg gcggactgtt
      841 cattgttttc taccagctgt ttgttcctgc cggcgaagcc atcaccggcg acggaatgaa
      901 aaaagcgatc gaagtcctcg ccggattttc actcggtgcg gaatccatcg cgctttttgc
      961 acgtgtcggc ggcggtattt acaccaaagc ggctgacgtc ggagcagatc ttgtcgggaa
     1021 agtggaagcg ggcatcccgg aagacgatgt gcgcaatccg gccaccattg cagataacgt
     1081 cggcgataac gtgggtgacg ttgccggaat gggagcggat ctcttcggct cctacgttgc
     1141 aaccatcctt gcaacaatgg tgctcgggca ggaactgaaa gcggacgacc agtttcacgg
     1201 tctgtcaccg atcctgcttc cgatggtcat cgccggaatg ggactggtat tttccattat
     1261 cggaactttt ttcgttcgca tcaaagatga aaaatcaagc gtgcaggcag cgctcaacat
     1321 gggtaactgg tcttcgatca ttctcacggc gatcgcttct tatttcctcg tcaactggat
     1381 gcttccggat gtgatgcact ggacaaccgc ttcccgcgga gctgatgtaa cgaaaggtgg
     1441 tgtgatcggg gcgatcatca cgggtctcat cgtcggaacg atcatgagcc tcgtgacgga
     1501 atattacacc gctatgggaa aacgcccggt gaaatccatc gtgcagaaat cggcaacggg
     1561 ccatgcgacc aacatcatcg gcggtctttc cgtcggaatg gaatctacgg ttattccgat
     1621 cctcgttctc gcggcgggaa ttatcgtttc gtattctttc gccagtcttt acggtgtggc
     1681 gatcgctgcg gcgggtatga tggcgactac ggccatgcag ctcgctatcg acgcgttcgg
     1741 tccgatcgcg gataacgccg gcggtattgc cgagatgtcc cagcttcccc cggaagttcg
     1801 tgagcgtacg gataatctcg atgccgtcgg gaatacgaca gctgctacag gtaaaggttt
     1861 tgcgatcgct tctgcggcgc tgacttcgct cgcgctgttt gccgcatttg tcggtattgc
     1921 cggcatcagc agcattgata tttacaaagc gcctgttctt gccgcgctct tcatcggggg
     1981 aatgatcccg ttcattttct cttctcttgc aattgctgct gtcggtcgtg ccgcaatgga
     2041 tatggtgaat gaagttcgcc gccagttccg cgagatcccc ggcatcatgg aatacaaagc
     2101 gaaacccgaa tatgaaaaat gcgtggcgat ctccacgaaa gcgtccatcc gcgagatgat
     2161 cgcgccgggt gctatcgcgc tgctcgttcc cgtactcgtc ggtttcggat tcaaaggtgt
     2221 tttcccggga acttcttccc cggaaatcct cggcggactt ctcgcaggtg taacggtttc
     2281 cggcgttctt atgggaatgt tccagaacaa cgccggcggt gcctgggaca atgcgaaaaa
     2341 atcattcgag aaaggcgtcg agatcaatgg cgaaaccttc tacaaaaaat cagatccgca
     2401 caaagcttcg gtaaccggtg acacagtggg cgatccgttc aaggacacat ccggtccttc
     2461 catgaatatt ctcatcaagc tgatgtccat cgtttcgctt gttatcgcgc cgcatatcgc
     2521 tgttccgaaa acggatgccg catgttcgat ggatggcgat cacaacaaat cgtgctgcat
     2581 gatgcagaac accaaatgcg atatggacaa gtgcatgcac atgagcaaag agcagtgtgc
     2641 gcattattgt gattcgatgg gctgttctgc ggaagaaaag gaaatgtgca tggagcacgc
     2701 cggaatgcag tgctgcaaag attcggcttc ctgcgatcat gacaaatgcg gcagcagctc
     2761 ctgcatgcct ggctgcacga atcattgttc ctcgaaagaa gaatgcgaaa agaactgcgg
     2821 agataaatgc gctgcaggcc ataaataaaa aagcttcccg gaattgggaa ccgctcctcg
     2881 ccggggcggt tcttttattt ccggggttct gtaatttgca aacgaattcc atcatcatgc
     2941 ctgagttcag cttcagcccg ttccccgttc tcgaaacaga acgtcttatc ctgcgccggg
     3001 tggaacccgc cgacgtagcc gaaatgtttg tcctgcgttc cgaccggcag ctgatgaaat
     3061 tcataccgcg gccggtggcc cagtcgcccg atgatgccct tgcgctcatt cattccatgg
     3121 cggagggaat cgcgaagaac gaaatgatca attggggaat ggtgctgaag agcagcggca
     3181 aagtaattgg cacggtcggt tttttccgga tgaagccgga acacttccgc gccgagatcg
     3241 ggtatatgct gcacggggat tttcacggaa cgggcatcat gcaggaagcg ctggtcaaag
     3301 cgatcgcatt cggatttcaa agcatgaagc tgcattcgat cgaagcagtg gtcgatcccg
     3361 caaacatcgc gtccatacgt ttgctggaac ggaacggttt tgtacgcgaa gcgtattttc
     3421 gtgaaaatga atttttcgat ggaaaattcc tcgactctgc agtgtattcc ctgcttaacc
     3481 gctgagtgat cctgcaaaaa aaaacgcagc agggaatttt gctgcgccgg ttcgcgaaaa
     3541 gaaattcgaa aacgtttatc agaagagccc gttgatctct gcgtcgatct tgttgatgat
     3601 cacgccgagg tgttcgggat tatcggagaa tttattttca tccacgtcaa cgatcaggag
     3661 cttgcccgct tcgtaagtgg agatccacgc ttcatatcgt tcattcaaac gcttcaggta
     3721 atcgatgcgg atcgcattct cataatcgcg gccgcgtttc tgtatctgcg aaacaagcgt
     3781 gggcaccgaa gcgcggaggt agatgatgag gtccggcggc gcgatgagcg attccatcag
     3841 gcggaaaagc gaaaagtaat tttcaaaatc acgcgtcgtc atcagtccca tcgaatgaag
     3901 gttgggcgca aagatgtgcg catcctcgta gatcgtgcgg tcctggatca cgttgcgttt
     3961 cgactggcgg atctcctgca cctgcgcgaa gcggctgttc aggaagaata tctggagatt
     4021 gaacgaccac cgctgcatgt cctcatagaa atcgctgaga tagggatttt cgtc
//
LOCUS       DASTSF010000100        13199 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_846440,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000100 DASTSF010000000
VERSION     DASTSF010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 13199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13199
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_846440"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..167
                     /locus_tag="VFU15_03020"
     CDS             <1..167
                     /locus_tag="VFU15_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010663305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HEU4716772.1"
                     /translation="LPGMKSPTILPLAEEGWCSVHSVLHEDEFWDIIESLKDNGAQGI
                     LVVPIEKMIV"
     gene            188..1468
                     /gene="hisD"
                     /locus_tag="VFU15_03025"
     CDS             188..1468
                     /gene="hisD"
                     /locus_tag="VFU15_03025"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEU4716773.1"
                     /translation="MILSEYPSPGKWETLLKRPAADVAQLEEKVSAIMHDVRMRGDEA
                     LLDYALKFDGVQPEGIAVRTDEMDAAERSLGAELRSAIMTAKSNIEKFHRAQEQPLLV
                     VETMPGVTCSRKSIPVEKAGLYIPGGTAPLFSTILMLAVPASIAGCREIILCTPPSKD
                     GTIHPAILFTAKACGITSVFCAGGAQAVAAMAYGTQTIPRVDKIFGPGNAWVTCAKQL
                     AAKEGIAIDLPAGPSELAVYADEHAPPAFVAADLLSQAEHGPDSQVVVVSTRREVLGN
                     ILREVETQLALLPRKETAERSLAKSHAILLENEEQVMKLMNAYAPEHLILACGNADAL
                     ADKVINAGSVFLGIWSPESAGDYASGTNHTLPTSGYARAWSGVSVDSFMKKVTFQKLT
                     REGLEAIGPVVETMAEAEGLFAHKNAVRVRLGGK"
     gene            1621..2673
                     /gene="hisC"
                     /locus_tag="VFU15_03030"
     CDS             1621..2673
                     /gene="hisC"
                     /locus_tag="VFU15_03030"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017258252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEU4716774.1"
                     /translation="MNIENLLRDNIRNLVPYSSARSEFSGNAEIFLDANENSFGSPLG
                     ENYNRYPDPLQLKVKEKISAIKGVPVQNIFLGNGSDEVIDLLFRAFCNPGKDSVIITP
                     PTYGMYEVSANINDVFVKRVPLTEDFQLDIEGISLASNEKTKLIFLCSPNNPTGNSLD
                     RGDVEIILNNFEGIVVIDEAYVNYSRQKSFIAELTEYPNLVVMQTLSKAWGLAGLRIG
                     MAFASEEITDVLNRIKPPYNISGIAQQAAAGALENISQVNAWIKETVSEREKLVREMG
                     KLPFVRKIYPSDANFLLVKMDDAPGVYHGLVGKGIIVRDRSKVILCEGCLRITVGTPE
                     ENRKLVEGLRGGRMEE"
     gene            2866..3225
                     /locus_tag="VFU15_03035"
     CDS             2866..3225
                     /locus_tag="VFU15_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018967989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HEU4716775.1"
                     /translation="MKTRTFKELIVWQKAHAFVLAVYKLTSSFRDDERFGLTQQFRRA
                     AVSIAANIAEGYKKRGLRDKARFYNISQGSLEECRYYLILSGDLGYGNPAVLLDDIEE
                     VSRILSSYQRSVVASAG"
     gene            3262..4374
                     /gene="hisB"
                     /locus_tag="VFU15_03040"
     CDS             3262..4374
                     /gene="hisB"
                     /locus_tag="VFU15_03040"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008240958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HEU4716776.1"
                     /translation="MKKKILFIDRDGTLIWEPPDTFQVDSFGKLKFLPYVISSLRKIA
                     AETDYELVMVTNQDGLGTASYPEETFWPVQNFIMQTLENEGIKFSEVCIDKSFPEENL
                     PTRKPGTGMLQNYLSGDYDLANSYVIGDRITDVRLAQNLGAKAIFIRNYDPAEGWEDK
                     ITFIAGDWNSIYEFLKRPPRKISHERKTKETDITVSLNLDGSGNSEIKTGLSFLDHML
                     DQLARHGNADLSISAKGDLHIDEHHTMEDTAIALGEAFAKALGDKRGIERYGFTLPMD
                     DCLAQVAVDFGGRPWLVWEAEFKREKIGDVPTEMFFHFFKSFSDAAKCNLNIKAGGTN
                     EHHKIEAIFKAFAKAIKMAVKRDKENDRLPSTKELL"
     gene            complement(4417..5511)
                     /locus_tag="VFU15_03045"
     CDS             complement(4417..5511)
                     /locus_tag="VFU15_03045"
                     /inference="COORDINATES: protein motif:HMM:NF013890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase family protein"
                     /protein_id="HEU4716777.1"
                     /translation="MLRKFLLLPALFLFLLPLRARHLQPGFDGNEFRKMFQVSAYQVD
                     TPWTKMWMAYPTGYTLIYRSPVSGLDNRWDLWKGDDSVDVISIRGTTASFDSWLENFY
                     AGLIPANGSLDMEKGKKFDYHLADDSSACVHIGWMIGLANIAPDVVEHINSEYKTGRR
                     EFCIMGHSQGGAIGFLLTSYLYYQKGKSIPADVVFKTYGSGTPKPGNLHYSYDFDFIT
                     RGGWALRVVNAADWVPEVPFTLQTLNDLNKGSPFTDASKLVKGANFFVKIYAKHLVRK
                     MNRSARRARKRFAHYLAKKSGKYVRKEINGFPKQQYEPSLNYAPCGVPIIMEPDAYYH
                     KLYPDDGKSIFRHHGFGPYLYLLNLYYPVT"
     gene            5778..6713
                     /locus_tag="VFU15_03050"
     CDS             5778..6713
                     /locus_tag="VFU15_03050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716778.1"
                     /translation="MKTIFGNEIPAEWKFIARPEGIGKVMFGFSSLREGKKVYTQTER
                     LAIRAGAAMIGAVGGTVAAFYTGSGTGATVAWVLVPALIFLGIAAGMTPSRFYNVFLG
                     TEGFAEYEVKADGLEPVPKGKIKFTADGHLIRKLVDHYKNGVYQYTAYTYRWVAGNGS
                     QLYRASHQYNKKTKHERVDRSKSYLGAVQYYYLNTAEQLWTAYLKEDADKKLQVNRNA
                     TFDFPIFSSDNRQEGKITLGQHMLTLEQNGQTLTFRDSELKQARFEKGTLYITDKEYR
                     WKLFGRQGNNIAIPVERIANCLFFLMIEKELLKIS"
     gene            6713..6982
                     /locus_tag="VFU15_03055"
     CDS             6713..6982
                     /locus_tag="VFU15_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEU4716779.1"
                     /translation="MKRVRIVVAGKVQGVFFRAETERTARSLGLSGFVQNQRDGTVYI
                     EAQGEEEKLQLLTGWCRQGPGRAVVTQITVEEIPLAEEMVFCIRR"
     gene            7217..8677
                     /gene="gatB"
                     /locus_tag="VFU15_03060"
     CDS             7217..8677
                     /gene="gatB"
                     /locus_tag="VFU15_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEU4716780.1"
                     /translation="MTASVYDKYEPVIGLEVHAQLLTHSKAYSSDAAEYGALPNTLVS
                     PVTLGLPGTLPMSNRRVIEYALRLGLATHCTIRERNEYARKNYFYADLPKGYQITQDK
                     TPICINGFITIKDADGNERKIRIHRIHMEEDAGKSMHDVDPTDTLVDLNRAGVPLLEI
                     VSEPDLRNADEAYSYLTEVRRLVRYLDICDGNMEEGSLRCDANISVMLKGAKEFGRKV
                     EVKNMNSISNVKRAIGHEIMRQIDIIEAGGIIDQETRSFDAVSGTTFSLRSKEMANDY
                     RYFPEPDLQPVIVSQDEIAHVKAHLPPLPAELVAKYTGEMGLSAYDAGVLTDSKEIAL
                     YFEEVLQYTTNAKAAANWVMGPVKSFLNENALHISKLAVTPARLAELIALVDSGKVNF
                     GVANQKIFPVMTKDASKTPQQIAEENNLVQESDSGALMGMIREAVAKYPAKVAEYKGG
                     KTGLLGMFMGEVMKLSKGKADPKVANELLKKVLDEQ"
     gene            8679..9881
                     /locus_tag="VFU15_03065"
     CDS             8679..9881
                     /locus_tag="VFU15_03065"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEU4716781.1"
                     /translation="MKRIVFSGLTATLLIFSFACKQRPKGNALITGQFTNCRNDTIYL
                     VDVSKSNFEVIDSVITGDDGSFELHPLFLYKGFYNLTVGKGSSQFATVILDAGDTISI
                     TGDAKNLGYTWKTKGSGDCTRFEELNNFISDIEKRKSPYMDRLDSIQQTFQYEVGLLP
                     KGDSLKVDSLDKVFGALYDSTQAKMKVLAAEGAAFVKSFIDKDPSSFANIPALRMIDP
                     FDGIDYYDRALEGLEVKYKNTPNVHLLRQYVEQERPFCKGQTPPEIALEDPNGQVIKL
                     SSLKGKVVLVDFWASWCGPCRAELPNVVANYKKYHDQGFEVFSVSLDSDKKAWTDAIA
                     KEGLVWPYHVSDLKEWQSSVVPLYRIKGIPKTLLLDRDGKILDRDLRGDALSKRLAGI
                     FTPAAPQK"
     gene            9933..13049
                     /locus_tag="VFU15_03070"
     CDS             9933..13049
                     /locus_tag="VFU15_03070"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEU4716782.1"
                     /translation="MKKQLLLSLSLFAAFAVSAKDYFQQDVAYTINVKLDDIHDELHG
                     NELLIYKNNSPDQLDFIYMHLWPNGYKDNNTPLGKQLKQDGSLFFYYAGDKDRGYIDS
                     LDFKVDGQPAKLEYVEGSPDIAKLILNTPLLPGGQISITTPFHVKIPSGKISRLGHIG
                     QQYQITQWYPKPAVYDKDGWEEIPYLNQGEFYSEFGTYDVFISLPKNYVLGATGDLQD
                     NPEEKKFIEDKVNETKAITAYDEKDDKFPASSDEWKTVHYHQDHVHDFAWFCDKRYHV
                     LKGEVTLPHTGKKVTTWIMYTNQYANLWKNAIEYMDSSVYYYSLWNGDYMYDVCTAVD
                     GALSAGGGMEYPNITVIGGVSSALELDVVITHEVGHNWFYGMLGSNERKHAWMDEGIN
                     SANELRYVATRYPNSPLIGGKGPIGKVSHVLGLEDYKRVAEYYQGYAFCARQNIDQPI
                     EMQSADFTSINYGTIVYMKSAIIFTYLRGYLGDSLYDACFQAYFDKWKLHHPMPEDIR
                     AVFEQVSGKDLSWLFVDLIDTNKKLDYKVCGVTDLLDEKKTKLAQGTDLKGNEFCVMV
                     KNCGEIKGPVCLSAIGDDGNILQTVWYDGFTGKVGLMFPKQSQHVHEFRIDGIEMMPE
                     VNRKNNTVRASGLFRKVEPLKLQIAGSLDDPRHSQLFWMPTIGFNKYDKFMIGAAFYN
                     HVVPGKHFEWLAMPMYSVGDNGLVGHADAFLNFNPNGPFQNIRIGASASSYDFHKVLS
                     SEYPLTPTPDRLLRFVKVAPELDLTIRKRIPRSPVTNSFRVRAVYLREDKIFNGFDYN
                     TGADSWYVSPVEKVIYEASYTFRDARSLDPFGFTLTYQNGDNMSKLQLTGDFKLNVAR
                     KKDILFRVFAGTFLSNSSDNDYRFRMSGFSPSAVSNQDYLYDNIFFGRTEASGFYHAQ
                     FVPEDGGFKMYSAAGQSDKFLAAVNVLIPMPGNNKLINRLNLYGDAGVYTATGVSGTE
                     FMYDAGVKLSLFRGGICDVYFPLVWSSQFTDYKKANGLKYGDLIRFTFILKQFNPSNI
                     TNLVRLGS"
BASE COUNT         3351 a         3553 c         3593 g         2702 t
ORIGIN      
        1 tcctgccggg aatgaagagc ccgacaattc ttccgctcgc cgaggaagga tggtgctccg
       61 tgcattctgt cctgcacgag gacgaattct gggacatcat cgaaagcctg aaagacaacg
      121 gcgcgcaggg aattcttgtt gtgcccatcg aaaaaatgat cgtctgatcc tgcaacaaaa
      181 gcgagctatg atactttctg aatatccttc accgggaaaa tgggaaacgc tgctgaaacg
      241 cccggccgcc gatgttgcgc agctggaaga aaaagtttcg gcgatcatgc atgacgtgcg
      301 catgcgtgga gatgaagcat tgctggacta tgcgctgaag ttcgacgggg tgcagccgga
      361 aggaattgcc gtgcgcactg atgaaatgga cgcggcggaa agatcgctcg gcgcggagct
      421 gcgttccgcg atcatgacag cgaaaagcaa cattgaaaaa tttcaccggg cccaggaaca
      481 gccgctgctt gttgtggaaa cgatgcccgg cgtgacctgc tcgcggaaga gcatcccggt
      541 ggaaaaagcg ggcctgtaca ttcccggcgg aacggccccg ctgttctcca ctattctcat
      601 gctcgctgtt ccggcctcga tcgcaggctg cagggaaatc attctctgca cgccgccttc
      661 gaaggacgga acgatccacc cggccatcct gttcactgca aaagcgtgcg gcatcacaag
      721 cgttttctgc gcgggcggtg cacaggcggt ggccgctatg gcgtacggaa cgcagacaat
      781 tccgcgcgtg gacaaaattt tcggcccggg aaatgcctgg gtgacgtgcg cgaaacagct
      841 cgcggcgaaa gaagggatcg cgatcgatct gccggccgga ccttcggagc tcgcggttta
      901 tgccgatgag catgcgcccc cggcattcgt tgccgccgac ctgctttcac aggcagaaca
      961 tggtcccgac agccaggtgg tcgttgtttc aacgcgcagg gaagtgctcg gaaacatcct
     1021 ccgcgaagtg gaaacgcagc ttgcgcttct tccgcggaag gaaacggcag aacgctcgct
     1081 cgcgaaaagc cacgccatcc tgctggaaaa cgaagagcag gtgatgaagc tgatgaacgc
     1141 ttatgcgccc gagcacctca tcctcgcctg cgggaatgcg gatgcgctgg cagacaaagt
     1201 gatcaatgcg ggttcggttt tcctcggcat ctggtcgccg gaaagcgccg gcgattatgc
     1261 atcgggaacg aaccatacgc tgcccacaag cggttatgcg cgcgcctgga gcggcgtttc
     1321 ggtcgacagc ttcatgaaaa aagtgacgtt ccagaagctc acgcgggaag ggctcgaagc
     1381 gatagggccg gtggtggaaa ccatggcgga agcagaagga ctttttgcgc ataaaaatgc
     1441 ggtccgcgtg cgcctgggcg ggaaatgata aaagacaaat gataaataaa aaaccgggga
     1501 tcaatgcgtg agggatagaa gcaaagtgcc gtgcactgcg gatagcccgg tgccgcgccg
     1561 ccaggtttgg cgcaggatga aacacaggcg cggcacacgc cccgaaaaag aaaaaacacg
     1621 atgaatatag aaaaccttct tcgcgacaac atccggaacc tggttccgta ttcttccgca
     1681 cgcagcgagt tcagcggcaa cgcggaaatt tttctcgatg cgaacgagaa cagttttggt
     1741 tccccgctcg gagagaatta caaccgttat cccgatccgc tacaactgaa ggtgaaggaa
     1801 aaaatatccg cgatcaaagg agtgcccgtg cagaatattt tcctcggcaa cggaagcgac
     1861 gaagtgatcg acctgctgtt ccgcgccttc tgcaacccgg ggaaagacag cgtcatcatt
     1921 acgccgccga cgtacggcat gtacgaggtg agcgcgaaca tcaacgacgt gttcgtgaaa
     1981 cgcgttccgc tgacggagga tttccagctg gacatcgaag gcatttcgct ggcgtcgaat
     2041 gaaaaaacga agctcatttt tctctgttcg cccaacaacc cgacggggaa ttccctcgac
     2101 cgcggggacg tggaaatcat cctgaataat tttgagggga tcgttgtgat cgacgaagcg
     2161 tacgtcaatt attcgcggca gaagagcttt atcgcggagc tgaccgaata cccgaacctc
     2221 gtggtgatgc agacgctgtc gaaagcatgg ggactcgccg ggcttcgcat cgggatggcc
     2281 ttcgcgtcgg aagagatcac cgatgtgctg aaccggatca agccgccgta caacatcagc
     2341 ggcattgcgc agcaggcggc cgcaggcgca ctggaaaata tttcgcaggt aaacgcatgg
     2401 ataaaggaaa cggtttcgga acgtgaaaag ctcgtgcgcg aaatgggaaa gctgccgttt
     2461 gtgcggaaga tctaccccag cgatgcgaat ttcctgctgg tgaaaatgga tgatgcgccg
     2521 ggtgtatatc atggccttgt cgggaaaggg atcattgtcc gcgaccgctc gaaagttatt
     2581 ctctgtgaag gctgcctgcg cattaccgtc ggaacgccgg aagaaaaccg gaagctggtg
     2641 gagggtttga ggggtgggag gatggaagaa taaaaggatg gaagaaggag aataaaagga
     2701 tggaagaatg gaaagatgag gatggaagaa ggagaataaa agaatggaag aatggaagat
     2761 tgtaagaagg agaatggaag aaggagaagg gaggatggag gatggagaga gtgcgggaat
     2821 tacaaaaacc taattaaaca taaagacaat actgttgctg aaaaaatgaa aacaagaaca
     2881 ttcaaagagt tgattgtatg gcagaaagcg catgcgtttg tgcttgccgt ttacaaattg
     2941 acttcatcat tccgggacga tgagcggttt ggcctgacgc agcaattccg gagagcggct
     3001 gtttcgatcg cagccaatat tgccgaaggc tacaagaaaa gaggtctccg cgataaggcg
     3061 cgtttttaca acatctctca aggttcgctg gaggaatgcc gctactacct gatactttcc
     3121 ggcgaccttg gatacgggaa cccggctgtc ctgctggatg acattgaaga agtgtcaagg
     3181 atattgagtt cctatcaacg ttctgtcgtc gcctcagcag gttaaagatt attccaacaa
     3241 ctctcccatt catatattcc catgaaaaag aaaatcctct tcatcgaccg cgacggaacg
     3301 ctcatctggg agccgccgga tacattccag gtcgacagtt tcggcaagct gaaatttctg
     3361 ccgtacgtga tctcttccct gcggaagatc gccgcggaaa cggattacga gctggtgatg
     3421 gtaacgaacc aggacgggct cggcacggcg tcctatcccg aagaaacatt ctggcccgtg
     3481 cagaatttta tcatgcagac gctggaaaac gaaggaataa aattcagcga agtatgcatc
     3541 gacaaaagtt tcccggaaga aaatctcccc acgcgaaaac cgggaacggg aatgctgcag
     3601 aactatcttt caggggacta cgacctcgcg aattcctacg tcatcggtga ccgcatcacg
     3661 gatgtcaggc tggcgcagaa tctcggcgcg aaagcgatct ttatccggaa ctacgatcct
     3721 gccgaaggct gggaagacaa gatcaccttc attgccggcg actggaattc catttatgaa
     3781 ttcctgaaac gcccgccgcg gaaaatttcg cacgaacgga aaacgaaaga aacggacatc
     3841 actgtttcgc ttaacctcga cggcagcggc aattcggaga taaaaacagg actttccttt
     3901 ctcgatcaca tgctcgacca gctggcgcgc cacgggaatg ccgatctctc gatctccgca
     3961 aaaggcgacc tgcacatcga tgagcaccac acgatggaag acacggcgat cgcgctcggc
     4021 gaagcatttg caaaagcgct cggcgacaag cgcggcatcg aacggtacgg ttttacgtta
     4081 ccgatggacg attgcctcgc gcaggtggcg gtcgatttcg gcggacgtcc gtggctggtg
     4141 tgggaagcgg aattcaaacg tgaaaagatc ggcgacgtgc cgacggaaat gtttttccat
     4201 tttttcaaat cgttcagcga cgcggcaaaa tgcaacctca acatcaaagc cgggggaacg
     4261 aacgagcacc acaagatcga ggcgatcttc aaggcgtttg caaaagcgat aaaaatggcg
     4321 gtgaagcgcg ataaggaaaa cgatcgcctc ccgagcacga aagaattgct gtgaagcaaa
     4381 atcccagagc aacgatcatc cgtcaacgac cggcattcag gttaccgggt aataaagatt
     4441 gagcaggtag agatagggcc cgaaaccatg atgacggaaa atgctcttgc cgtcgtcggg
     4501 ataaagtttg tggtagtatg catcgggttc catgatgatg ggaacgccgc agggcgcata
     4561 gttcaggctc ggctcgtatt gctgcttcgg aaaaccgttg atctctttcc gcacgtactt
     4621 ccctgatttt ttcgcgaggt aatgcgcaaa acgtttccgc gcccgccgtg cagaacggtt
     4681 cattttgcgg acgagatgct tcgcatagat cttcacaaaa aagttggcgc ctttcaccag
     4741 cttgctcgca tcggtgaacg ggcttccctt gttcaggtcg ttcagcgtct gcagcgtgaa
     4801 cggaacttcc ggcacccagt cggctgcatt caccacgcgc agcgcccacc cgccgcgtgt
     4861 gatgaaatcg aaatcgtagc tgtaatgcag gttgccgggt ttcggtgtgc cgctcccgta
     4921 tgttttgaaa acgacgtcgg ccgggatgga ttttcctttc tggtaataga ggtacgacgt
     4981 cagcaggaaa ccgatcgcgc ctccctggct gtgccccatg atgcagaatt ccctgcgccc
     5041 tgttttgtat tcgctgttga tgtgctccac cacgtcaggt gcgatgttcg cgaggccgat
     5101 catccagccg atgtgcacgc aggcggaaga atcgtcggcg agatggtagt caaacttttt
     5161 ccctttttcc atatccaggc tgccgttcgc cggaatgagt cccgcgtaaa aattttccag
     5221 ccacgaatca aatgatgccg tcgttccgcg tatgctgatc acgtccaccg aatcatcgcc
     5281 cttccaaaga tcccaccggt tatcgagtcc cgaaaccggc gagcggtaaa tgagcgtgta
     5341 gcccgtggga taggccatcc acattttcgt ccacggcgta tcgacctggt aagcggaaac
     5401 ctggaacatt ttccggaatt catttccgtc gaaaccgggt tgcagatgac gcgcacggag
     5461 cggaagcaga aaaaggaaaa gggcgggaag aagcaaaaat tttctgagca tgaagtaaag
     5521 gtaatttgaa atcggaaaaa gcttcccgga cccggaacta aaaaccattt caaccacgga
     5581 gagcacaaac attttctcaa agtgtgcgaa gacaattcag ttggaaagtt gaaatctgga
     5641 aagtcaaaac cacgaagaac acaaagcatt ttcacaaagc gcacaaagga aaacaaaagc
     5701 aacccgaaac tttaaacttt ccaactttcc cccaatccct caatttccta tctttccgtt
     5761 cccacaaaaa acctccaatg aaaacaatct tcggaaacga aatccccgcc gaatggaaat
     5821 tcatcgcgcg ccccgagggc atcgggaaag tcatgttcgg attctcttcc ctccgcgaag
     5881 gaaaaaaagt gtacacacag actgaacggc tcgccatccg ggccggcgcc gctatgatcg
     5941 gtgccgtagg tggaacggtg gccgcatttt acaccgggag cggaaccggc gccaccgtag
     6001 cctgggtcct ggttccggcg cttatctttc tcggcatcgc cgccggcatg acgccgtcca
     6061 ggttctataa cgttttcctc ggcacggaag gattcgccga atacgaggtt aaagccgacg
     6121 ggctcgagcc ggtgccgaaa ggaaaaataa aattcaccgc cgacggtcac ctcatccgca
     6181 aacttgtcga ccattacaag aacggcgttt accagtatac ggcctacacc tatcgctggg
     6241 tggccggcaa cggttcgcag ctgtaccgcg catcgcacca gtacaacaag aaaacaaagc
     6301 acgaacgcgt cgaccgctcg aaaagctacc tcggtgctgt gcagtattat tacctcaaca
     6361 ccgctgaaca gctctggacg gcttacctga aagaagatgc cgacaaaaag ctgcaggtca
     6421 accgcaacgc cacgttcgat tttcccatct tcagcagcga caaccgccag gaaggaaaga
     6481 tcaccctcgg ccagcacatg cttacgctcg agcagaacgg gcagacgctt acgttccgcg
     6541 attcggagct gaagcaggcg cgcttcgaaa aaggcaccct ctacatcacc gacaaagaat
     6601 accgctggaa gctcttcggc cgccagggaa acaacatcgc cattcctgtc gagcgcatcg
     6661 ccaattgtct tttcttcctc atgatcgaga aggaactgct gaagatcagc tgatgaaacg
     6721 cgtccgcatc gtcgtcgccg gaaaagtgca gggcgttttt tttcgcgcgg aaacagagcg
     6781 cacggcgcgc agcctcgggc tttccggttt cgtgcagaac cagcgcgacg gcacggtgta
     6841 catcgaagcg cagggggagg aagaaaaact gcagctgctc accggctggt gccggcaggg
     6901 acccgggcgg gctgtcgtca cgcaaataac ggtggaggaa attccattgg cggaggaaat
     6961 ggttttttgc atccggcgct gatcatgcag cgcacctcct gttctttttt gttccggctt
     7021 tcccgtgctc gctgttacat ggtaatcgct cgcccggggc tggttgtgtt acgtcatcgc
     7081 tctgtatctc ccgtcccgtg cccggcattc ggtatctcgc gtctcttaac acattctcac
     7141 attacgccgc cctccggcat cacattaccc cattgatttt ccgtaatttc gcttctcaat
     7201 tcgcgtttcc cgttaaatga ccgcatccgt ctacgacaaa tacgagcctg ttatcggcct
     7261 tgaggtccac gcacagctgc tcacgcacag taaagcctat tcatccgacg ccgccgaata
     7321 cggcgcgctc cccaacacac tggtaagtcc cgtcacgctc ggacttccgg gcacgcttcc
     7381 catgtcgaac aggcgcgtga tcgaatacgc gctgcgtctc ggtcttgcca cgcactgcac
     7441 gatccgcgaa cggaatgaat acgcacgaaa gaattatttc tacgccgatc ttcccaaagg
     7501 gtaccagatc acgcaggaca aaacgccgat ctgcatcaac ggcttcatca ccatcaagga
     7561 cgctgacggg aacgagcgta agatccgcat ccaccgcatt cacatggaag aggacgccgg
     7621 caagagcatg cacgacgtcg atcccaccga cacgctcgtc gacctcaacc gcgcgggcgt
     7681 tccgctgctg gaaattgtca gcgagcctga tctccgcaat gcggatgaag cgtacagcta
     7741 tctcaccgaa gtgcgccgac tcgtgcgcta cctcgacatc tgcgacggca acatggaaga
     7801 aggctcgctc cgctgcgacg cgaatatttc tgtcatgctg aaaggcgcga aggaattcgg
     7861 ccgcaaggtg gaagtgaaga acatgaattc catttccaac gtgaagcgcg ccatcgggca
     7921 cgagatcatg cgccagatcg atatcatcga ggcgggcggc atcatcgacc aggaaacgcg
     7981 cagcttcgac gcggtgagcg gcacgacatt ctcgctgcgt tcgaaggaaa tggccaatga
     8041 ttaccgctat ttccccgaac ccgatctgca gcccgtgatt gtttcgcagg acgagatcgc
     8101 gcacgtgaag gcgcacctgc cgccgcttcc tgcggaactc gttgcgaaat acaccggcga
     8161 aatgggactt tccgcttacg atgcgggcgt gctcaccgat tcgaaagaga tcgccctgta
     8221 tttcgaggaa gtgctgcaat acacaacgaa tgcgaaagcc gccgccaact gggtcatggg
     8281 cccggtgaaa tcattcctca atgagaacgc gctgcatatc tcgaagctcg ccgtcacgcc
     8341 ggcccgtctt gcggagctga tagcgctcgt cgattcgggc aaggtcaatt tcggcgtcgc
     8401 caaccagaag atattcccgg tgatgacgaa agacgcttcg aaaacaccgc agcagatcgc
     8461 ggaagaaaac aaccttgtgc aggaaagcga ttccggcgcg ctcatgggaa tgatccgtga
     8521 agccgtcgca aaatacccgg cgaaagtcgc cgaatacaaa ggcggaaaaa ccggtctgct
     8581 cggcatgttc atgggcgaag tgatgaagct ctcgaaagga aaagccgatc cgaaagtggc
     8641 caatgaactt ctcaaaaaag tgctcgatga acaataacat gaaacggatt gttttctctg
     8701 gattgacggc aacgctgctg atcttctcct tcgcctgcaa gcagcgcccg aaaggaaatg
     8761 cgctcatcac gggccagttt accaattgca ggaacgacac gatctacctc gtcgacgtca
     8821 gcaaaagcaa tttcgaggtg atcgattccg tcatcaccgg tgacgacggc tccttcgagc
     8881 tgcacccgct cttcctctac aaaggttttt ataacctcac cgtcgggaaa ggctccagcc
     8941 agttcgctac cgtcatcctc gatgcaggcg atacgatctc cattaccggc gacgcgaaga
     9001 atctcggcta cacatggaag accaaaggct ccggcgactg cacgcgtttc gaagagctga
     9061 ataatttcat ttcggacatc gaaaaaagaa aatcgccgta catggaccgc ctcgattcca
     9121 tccagcagac gttccagtat gaggtcggcc tgctgcccaa aggcgacagc ctgaaagtcg
     9181 attcgctcga caaagtgttc ggtgcgctgt acgattccac gcaggcgaaa atgaaagtgc
     9241 tcgccgccga aggtgccgca ttcgtgaaat cgttcatcga caaggatcct tcttcgttcg
     9301 ccaacattcc cgcgctgcgc atgatcgatc cgttcgacgg catcgactat tacgaccggg
     9361 cgctcgaagg tctcgaagtg aaatacaaaa acacgcccaa cgttcacctg ctgcgccagt
     9421 atgtagagca ggaacgcccg ttctgcaaag ggcagacgcc gcccgagatc gcgctcgaag
     9481 atccgaacgg gcaggtcatc aagctttctt cgctcaaagg gaaagtcgtg ctcgtcgatt
     9541 tctgggcgtc ctggtgcggg ccctgccgcg cggaattacc caacgtcgtg gcgaattaca
     9601 aaaaatacca cgaccaggga ttcgaagtgt tttccgtttc gctcgacagc gacaaaaaag
     9661 cctggacaga tgcgatcgcg aaagaaggtc ttgtgtggcc ttaccacgtg agtgatctca
     9721 aggaatggca gtcgtctgtc gtgccgctgt accgcatcaa gggaattccc aaaacgctgc
     9781 tgctcgaccg tgacggaaag atcctcgacc gtgatctccg cggtgatgcg ctcagcaagc
     9841 gcctggccgg aattttcacg cctgccgctc cgcagaaata aattgtaact aaccgatagt
     9901 tcttcacgtc ccagtcgaaa aactgacctg gtatgaaaaa acaactactg ctttctcttt
     9961 ccctttttgc tgcattcgca gtttccgcca aggattattt ccagcaggac gtcgcgtaca
    10021 cgatcaacgt gaagctcgac gacatccatg acgaactgca cgggaacgaa ttgctcatct
    10081 ataagaacaa ctctcccgac cagctcgatt tcatttacat gcacctctgg ccgaacggtt
    10141 acaaagacaa caatacgccg ctcgggaaac agctgaagca ggacggaagc ctgttctttt
    10201 attatgccgg cgacaaggac cgcggttaca tcgacagcct cgatttcaaa gtggacggtc
    10261 aaccggcgaa gctcgaatat gtcgaaggaa gtcctgacat cgcaaagctc attctcaata
    10321 cgccgctcct gcccggcgga cagatctcca ttactactcc tttccacgtg aaaattccca
    10381 gcggaaaaat ttcgcgtctc ggtcacatcg gccagcaata ccagatcacg cagtggtacc
    10441 cgaaaccggc agtgtacgac aaggacggct gggaagaaat tccgtacctc aaccagggcg
    10501 aattttattc cgaattcggc acgtacgacg tgttcatctc gctcccgaaa aattatgtcc
    10561 ttggcgcaac cggcgatctg caggacaatc ccgaagagaa aaaattcatt gaggataaag
    10621 tgaacgaaac gaaagcgatc accgcctacg atgaaaaaga cgataaattc cccgcttcct
    10681 cggacgaatg gaaaaccgtg cattaccacc aggaccatgt gcacgatttc gcatggttct
    10741 gcgacaagcg gtaccacgtg ctgaaaggag aagtcacgct gccgcacacc ggcaagaaag
    10801 tgaccacctg gatcatgtac acgaaccagt acgccaacct ctggaaaaat gcgatcgagt
    10861 acatggacag ctccgtttat tattattcgc tgtggaacgg cgattacatg tacgatgtct
    10921 gcacggctgt cgacggtgcg ctgagcgcag gcggcgggat ggaatacccg aacatcaccg
    10981 tgatcggcgg tgtgagcagc gcgctcgagc tcgatgtggt gatcacgcac gaggtcgggc
    11041 acaactggtt ctacgggatg ctcggcagca acgagcgcaa gcacgcatgg atggatgaag
    11101 ggatcaactc ggcgaatgag ctgcggtacg tggcgacgag gtacccgaac tcgccgctga
    11161 tcgggggaaa gggacccatc gggaaagtgt cgcatgtgct cgggctggaa gattacaagc
    11221 gcgtggcgga atattaccag ggttatgctt tctgtgcaag acagaacatc gatcagccca
    11281 ttgaaatgca gtcggcggat ttcacctcca tcaattacgg cacgatcgtt tatatgaaga
    11341 gcgccatcat tttcacgtac ctgcgcggct acctcggcga ttcattgtac gatgcctgtt
    11401 tccaggcgta tttcgataaa tggaaattgc atcacccgat gccggaggac atccgcgctg
    11461 tgtttgagca ggtgtcggga aaagatctca gctggctttt tgtcgacctg atcgacacga
    11521 ataaaaagct ggattataaa gtttgcggag taacggatct gctcgatgaa aaaaagacga
    11581 agctcgcgca gggaacggat ctgaaaggca acgagttttg cgtgatggtg aaaaactgtg
    11641 gggaaataaa aggacccgtt tgcctgagcg cgatcgggga tgatggaaat attctgcaga
    11701 ccgtgtggta tgatggtttc accggcaagg tcgggctgat gttcccgaaa cagtcgcagc
    11761 acgtgcatga attcaggatc gacggtatcg aaatgatgcc cgaagtgaac cggaagaaca
    11821 acacggtgcg cgcttccggg ctcttccgga aagtggagcc gctgaagctg cagatcgccg
    11881 gaagtctcga tgatccgcgc cattcgcagc tgttctggat gcccacgatc ggcttcaata
    11941 aatacgacaa gttcatgatc ggcgcggcat tctacaatca cgtcgttccc ggcaagcatt
    12001 tcgaatggct ggccatgccg atgtattccg tcggcgacaa cggcctcgtg ggtcatgcgg
    12061 atgcattcct gaacttcaat cccaacggcc cgttccagaa tatccgcatc ggcgccagcg
    12121 cttcctcgta cgattttcac aaggtgcttt cgtccgaata cccgcttacc ccaacgccgg
    12181 atcgtcttct tcgctttgtg aaagtggcgc ccgagctgga tctcacgatc cgcaaaagaa
    12241 ttccccgcag cccggtcacg aattctttcc gcgtgcgcgc cgtttacctg cgcgaggata
    12301 aaattttcaa cgggtttgat tacaacacgg gcgcggatag ctggtatgtt tctcctgtcg
    12361 aaaaagtaat ttacgaagcg agctacacgt tccgcgatgc gcgcagcctc gatccgttcg
    12421 gatttacgct tacgtaccag aacggcgata atatgtcgaa gctgcagctg acgggtgatt
    12481 tcaagctcaa cgtggcgcgt aagaaagaca tactgttccg tgtatttgcg gggacgttcc
    12541 tcagcaacag cagcgataat gattaccgtt tccgcatgag cggtttttcc ccgtcggctg
    12601 tttccaacca ggattatctc tacgacaata ttttcttcgg ccgcacggaa gcatccgggt
    12661 tctaccacgc gcagttcgtt ccggaagacg gcggattcaa aatgtattcg gccgccgggc
    12721 agagcgataa attcctcgca gcggtgaacg tgctcattcc catgccgggg aacaacaagc
    12781 tgatcaatcg cctcaatctt tacggcgatg cgggcgttta caccgcgacc ggtgtgagcg
    12841 gaacggaatt tatgtacgac gcgggtgtga agctttcgct gttccgcggc ggaatctgtg
    12901 acgtttattt cccgctcgtc tggtcttcgc agttcaccga ttacaagaag gcgaacgggc
    12961 tgaaatacgg cgacctcatc cgctttacgt ttatcctgaa acaattcaac ccgtcgaaca
    13021 tcaccaacct ggtacgcctc ggatcatgag tccagacaac gggagtaaaa tatatttcgc
    13081 ttccgatttt catttcggga taccggacca tgcggaaagt cttgtgcgtg aaaaacgcgt
    13141 gatccgctgg atggattcca tcaaagagga aacagccgag ctgttcctcg tcggcgacc
//
LOCUS       DASTSF010000101         2972 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1946392,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000101 DASTSF010000000
VERSION     DASTSF010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2972)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2972)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2972
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1946392"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2166
                     /locus_tag="VFU15_03075"
     CDS             <1..2166
                     /locus_tag="VFU15_03075"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEU4716783.1"
                     /translation="KGIFDQQFLARVQDLTDTLRKLPDVTKVISPSDLTYPTTTGEYP
                     YLHTDDPSRFKTDSAIIYRSKELVGSFFSRNAKSVCLYIKTTEGISKIKSDTLLARMD
                     RQVAREHFDAVHSASKMRGQKVYLDRLQREFFIFFISSFLLVVLFLFISFRSFWGIWV
                     PIFVVLLSILWLLALMTALGKALDIMTVLIPTMMFVVGMSDVVHIVTKYLEELRDEER
                     HFGKANRFNALLKTIEDVGFSTFITLITTSLGFLTLINSHILPIRDFGLYTSIGVFIA
                     FILAFTIMPLVLNVIPQPNLKLEHETSRFWTKRLHSMLLWIFRNGKMIGAVTVVLTLL
                     SLWGINSIERNNYLIEGLTRKDELRKDFFYFENNYSGVRPFEMVISPADSTHTVLGVD
                     ELRAADHLEDYLQKKFGVGFLLSPLSMVKEANKAENNGDPDYYVLPKDSSDIADLASE
                     ILSHKKRKSVRHFDTAQRSICSYAAYFAVYGKKNTRAGRNDKNELSLFISDDAKEGRI
                     SGKMYDVGSKVIRERTAGLDHFLATDPQMKLLHVKMTGAAVMLDKNNEYLISNMLQGL
                     FLSVFVVGLILGIIHRSWKMGIIAIIPNFLPILFIGGIMGLFGVELNSSTSIIFSIAF
                     GIATDDTVHFLGRLKLERKKGMPLFLALKRTYLSTGKAVIVTSLILSAGFFTLIASGF
                     QSTFLFGLLVSVTLIIAVMTDLLLFPLLIVWMRPKIKAG"
     gene            complement(2198..2587)
                     /locus_tag="VFU15_03080"
     CDS             complement(2198..2587)
                     /locus_tag="VFU15_03080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716784.1"
                     /translation="MRTIYFLPAVMAFVIAACRAGSGGDATIKAVLKHHDRVIPSQQN
                     YPDSIFIKFNASDLPADIETHYDLLFTGADGDDHVNCTGLKAGQYYIFATGWDTAISQ
                     RVSGGLAVKLKWKQRKTETDVDVPVTE"
     gene            complement(2598..>2972)
                     /locus_tag="VFU15_03085"
     CDS             complement(2598..>2972)
                     /locus_tag="VFU15_03085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716785.1"
                     /translation="TIHYGNAFNYCLSIGFLAYPYRYKSYKDVNINLYAEFYGKDYQA
                     AKIYSDGQVIPVSGAPTLDGGNYVEARPAIQFIFKSNFRVDLCYGMKMIGRSYVNTYP
                     LYMFNIQYYFFPKIKKYAYDQA"
BASE COUNT          673 a          835 c          746 g          718 t
ORIGIN      
        1 aaagggattt tcgaccagca gttcctcgcc agggtgcagg acctcaccga cacgctgcgc
       61 aagctgcccg acgtcacgaa agtcatttca ccgtccgatc tcacttatcc caccaccacg
      121 ggcgaatatc cgtacctgca caccgacgat ccttcgcgtt tcaaaacgga ttccgccatt
      181 atttatagat cgaaagagct ggtcggttcg ttcttttcac gcaacgcgaa atccgtttgc
      241 ctctacatca agacgacgga aggaatttcg aaaataaagt ccgacacgct gctcgcccgc
      301 atggaccggc aggtggcgcg ggaacatttc gacgcggtgc attccgccag caagatgcgc
      361 gggcagaaag tttacctcga ccggctgcag cgggaattct tcattttctt catctcttcg
      421 ttcctgctcg ttgtcctttt cctgttcatc tcgttccgct cgttctgggg aatatgggtg
      481 ccgatcttcg tcgtgctgct gtccatcctc tggctgctcg cgctgatgac agcgctggga
      541 aaagcgctcg acatcatgac cgtcctcatc ccgacgatga tgttcgtcgt gggcatgtcg
      601 gatgtcgtgc acatcgtcac gaaatacctc gaagaattgc gcgacgagga gaggcatttc
      661 ggcaaagcga accgcttcaa tgcattgctg aaaaccattg aagacgtagg tttctcgacg
      721 ttcatcacgc tcatcaccac ctcgctcggg ttccttacgc tcatcaattc gcacatcctt
      781 cccatccgcg atttcgggct ctacacgtcg atcggcgttt tcatcgcgtt cattctcgcg
      841 ttcacgatca tgccgctggt gctgaacgtc attccgcagc ccaacctgaa gctcgagcac
      901 gagacgagcc ggttctggac gaaacgcctg cacagcatgc tgctgtggat cttccgcaac
      961 gggaaaatga tcggcgccgt aacggtcgtt ctcacgctgc tcagtctctg gggcatcaac
     1021 agcatcgaac gcaacaatta cctcatcgaa gggttgacgc gcaaagacga gctgcggaag
     1081 gattttttct atttcgagaa taattattcc ggcgtgcgtc cgttcgaaat ggtgatctcg
     1141 cctgcagatt ccacgcatac ggttctcggc gtggacgagc tgcgcgcagc ggatcatctc
     1201 gaagattacc tgcagaaaaa attcggtgtg ggattcctgc tgtcgccgct ttccatggtg
     1261 aaggaagcga acaaagcgga aaacaacggc gatcccgatt actatgttct tcccaaagac
     1321 agcagcgaca tcgccgacct ggcgtcggaa attctttcgc acaaaaagag aaaaagcgtt
     1381 cgtcatttcg acacggcgca gagaagcatc tgcagctatg ccgcgtattt cgccgtttac
     1441 ggaaagaaaa atacgcgggc cggaaggaac gacaagaacg aactgagcct tttcatttcc
     1501 gacgacgcga aagaaggacg gatctccggg aaaatgtatg acgtgggcag caaggtcatc
     1561 cgcgaacgga cagcaggcct cgatcatttt ctcgccaccg atccgcagat gaagctgctg
     1621 cacgtgaaaa tgacgggcgc cgcggtgatg ctcgacaaga acaacgaata cctcatctcg
     1681 aacatgctgc agggactttt cctttcggtg ttcgtcgtgg gactgatcct cgggatcatt
     1741 caccgctcgt ggaagatggg catcatcgcc attatcccga acttcctgcc cattcttttc
     1801 atcggcggca tcatgggact tttcggcgtg gagctgaatt cttccacgtc gatcatcttc
     1861 tccatcgcct tcggtatcgc caccgacgac accgtgcatt ttctcgggcg cctcaagctc
     1921 gaacgcaaaa aaggcatgcc gcttttcctc gcgctcaagc gcacgtatct atccaccggc
     1981 aaagccgtga tcgtgacgtc gctcattctc agcgcgggat ttttcaccct catcgcttcc
     2041 ggtttccaga gcacgttcct cttcgggctt ctcgtcagcg tcacgctgat catcgccgta
     2101 atgacggacc tgctgctttt cccgctgctc atcgtgtgga tgaggccaaa aataaaggcc
     2161 gggtgaattc ccggcctcca tcgatctgta ctgaaaatta ttctgtcacc gggacgtcca
     2221 catcggtttc ggtcttacgc tgtttccact tcagcttcac ggcaagaccg ccgctcacac
     2281 gctggctgat cgccgtgtcc cagcctgttg caaagatgta atactgcccg gctttaagcc
     2341 ctgtgcagtt cacgtgatca tccccatccg ctccggtaaa aaggaggtca taatgcgttt
     2401 cgatatccgc cggaagatcc gacgcattga acttgataaa aatgctgtca ggataattct
     2461 gctggctggg aatgaccctg tcgtggtgct tcagaacggc ttttatcgtg gcatcgccgc
     2521 cgcttcccgc tctgcaggct gcaatgacga aagccatcac tgccggaagg aaatagattg
     2581 tccgcattgt gtttttttta agcctgatca tacgcatact ttttaatttt cggaaagaag
     2641 taatactgga tattgaacat gtacagcggg taggtattga cgtacgagcg gccgatcatt
     2701 ttcatcccgt agcagagatc gacacggaaa ttggacttga aaatgaactg gatcgcgggc
     2761 cgcgcctcga cgtaatttcc accgtccagc gtgggggcgc cgcttaccgg gatgacctgg
     2821 ccgtccgaat aaattttcgc agcctggtaa tcttttccgt aaaactccgc atacaggttg
     2881 atgttgacat ccttataact tttgtacctg taaggatacg cgagaaaacc gatggaaagg
     2941 caataattga atgcgtttcc atagtgaatg gt
//
LOCUS       DASTSF010000102         3062 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_826690,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000102 DASTSF010000000
VERSION     DASTSF010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3062
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_826690"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1480)
                     /locus_tag="VFU15_03090"
     CDS             complement(<1..1480)
                     /locus_tag="VFU15_03090"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011585904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEU4716786.1"
                     /translation="MSATKYVFVTGGVSSSLGKGIVSASLAKLLQARGYSVTIQKLDP
                     YINVDPGTLNPYEHGECYVTDDGAETDLDLGHYERFLNAPTSQANNVTTGRIYKTVIE
                     KERRGDYLGKTVQIIPHITDEIKHRIRLLGNTGQYQVIITEIGGTVGDIESLPYIEAV
                     RQLRWEMPKDVLVVHLTLVPYLSASGELKTKPTQHSVKLLQEYGVTADVLVCRTEHPL
                     NDDLKRKVALFCNVDNNAVIEAMDAKSIYEVPLLMEKEKLDVVVLQKLGLSSEKEPDM
                     ARWKDFLGKLYHPASEVQIGLIGKYIELKDSYKSIAESFIHAGVANDCKVNVQWIHSE
                     SIDDSNVSEKLSGLAGILVAPGFGHRGIEGKISAIRFARENNIPFLGICLGMQCAVIE
                     FARNVLGMKGAHSTEMDPNTPYPVIDLLEAQKKVTHKGGTMRLGAYPCELADGSLASS
                     VYGRHTIYERHRHRFEFNNEFLQDYERAGMMATGINPEGGLVE"
     gene            complement(1604..>3062)
                     /locus_tag="VFU15_03095"
     CDS             complement(1604..>3062)
                     /locus_tag="VFU15_03095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716787.1"
                     /translation="FMFNDSVDHYKNSVDLSWKSVGFGSNFQVIPGGSNTIIKGNFAY
                     SRYGIGMSEDYTNRRHSEVDGFNMGLNFTYFQRKNVVNYGLEILGFKTDFDFINPVNR
                     EISQIDNTTEFAAYFKYKWISKNMAKDSLANHTLVVEPGFRFQYYASLNNASPEPRLT
                     FKYNVSNHFRIKGATGMYSQNLLSAVSDRDVVNLFYGFLSGSDNIPTQFTDQNGNTYQ
                     ITSKLQKANHYIFGFEYDPIDTIEKWGNLEFTLEGYLKDFRQLTNLNRNKLYDDTPAN
                     SDKPDYEKKDFIVETGKAYGVDFLAKYEYRDFYFWGVYSLGYVTRWDGTELYEPNFDR
                     RHNINIVSTYDFGKRISKLDSTTGKWVKTEYRKKNWQVSIRWNFGSGFPFTPTQGFFE
                     NITFSTITQNYTTQNGSLGIMYGDLNSKRLPTYHRLDVTIKRTWEFSDISTLEVALSA
                     TNIYDRENIFYINRVTQQRVNQLPIMPSLSMNWSF"
BASE COUNT          658 a          762 c          844 g          798 t
ORIGIN      
        1 tctcgacaag gccgccttcc gggttgatcc ccgttgccat catgccggcg cgctcgtaat
       61 cctggagaaa ttcattgttg aactcgaaac ggtgacggtg ccgctcgtaa atcgtgtgcc
      121 tgccatagac agatgaggca agcgacccat cggccagctc gcacgggtaa gcgccgagcc
      181 gcatggtacc gcctttgtgc gtaacctttt tctgcgcttc gagcagatcg atcacgggat
      241 aaggcgtgtt cggatccatt tccgtcgagt gggcgccttt catgccgagg acgttccgcg
      301 caaattcgat cacggcgcat tgcatgccga ggcagatgcc gaggaacggg atgttgttct
      361 ctcgcgcaaa acgtatggcg gagatttttc cctcgatgcc gcggtgaccg aagcccggtg
      421 cgacaagaat gccggcgagg cccgaaagct tttcgctcac attgctgtcg tcgatgcttt
      481 cggaatggat ccactgcacg ttcactttgc agtcgtttgc aacgcccgca tgaatgaagg
      541 attccgcaat ggatttatag gaatctttca gctcgatgta tttcccgata agcccgatct
      601 gtacttcgga agcagggtgg tacagcttgc cgagaaaatc cttccagcgc gccatgtcgg
      661 gttctttttc cgaggaaagt cccagcttct gcagcaccac gacatccagc ttttcttttt
      721 ccatcagcag cggaacctcg taaatgcttt tcgcatccat cgcttcgatg accgcattat
      781 tgtcgacgtt gcagaacagc gctactttgc gtttcaggtc gtcgttcagc ggatgttcgg
      841 tacggcacac cagcacatcg gccgtaacgc cgtattcctg gagcagcttt acggaatgct
      901 gcgtcggttt tgttttcagc tccccggaag cggagaggta aggcaccagc gtgaggtgca
      961 ccacgagtac gtctttcggc atttcccagc gcagctggcg aaccgcttcg atgtagggaa
     1021 gtgattcgat gtcgcccacg gtgccgccga tctcggtgat aatgacctgg tattgtcctg
     1081 tatttccaag gagccgtatg cggtgtttta tttcgtcggt aatgtgcggg atgatctgca
     1141 cggttttccc gagataatcg cccctgcgtt ccttttcaat aacggtcttg tagatccttc
     1201 ccgtagtgac gttgttcgcc tgggaagtgg gtgcgttgag aaaacgctcg taatggccca
     1261 ggtcgagatc ggtttccgcc ccgtcgtcag tcacataaca ttcaccgtgc tcgtatgggt
     1321 tcaatgtccc ggggtccacg ttgatgtaag gatcgagctt ttgtatggtg acggaatatc
     1381 ccctcgcctg caaaagcttg gcaagtgacg cggaaacgat gccctttccc aatgaagagg
     1441 acacaccccc ggttacgaag acgtatttag tggctgacat aagaagagga attgaacggt
     1501 aaccgggaaa caaaggtacg gaactccgtc cacttgctta aacggcgtta acagtttgtt
     1561 caacactttt ttcagcaatt acaaatcact gaaaaacagg aactcagaac gaccagttca
     1621 tgctcaggct gggcatgatg gggagctggt tcacgcgctg ctgcgtcacc cggttgatat
     1681 agaagatgtt ttcccggtcg taaatgttgg ttgcgctcag cgcgacttcc agcgtggaga
     1741 tgtcgctgaa ttcccacgtt cttttgatgg taacgtcaag acggtgatac gtgggaagac
     1801 gcttggagtt gaggtctccg tacatgatgc ccagcgatcc gttctgcgtg gtgtaattct
     1861 gggtgatggt gctgaacgtg atgttctcga aaaagccctg cgtaggcgtg aacgggaagc
     1921 ctgagccgaa attccagcgg atggaaacct gccagttctt tttgcggtat tctgttttca
     1981 cccatttgcc ggttgtgctg tcgagcttcg aaatgcgttt tccgaaatca taggtggaaa
     2041 cgatgttgat gttgtggcgg cggtcgaagt tgggttcata aagctccgtt ccgtcccagc
     2101 gcgtcacata gccgagcgaa taaacgcccc agaaatagaa atcgcggtat tcgtatttcg
     2161 caaggaaatc aacgccataa gctttcccgg tttccacgat gaagtctttc ttctcgtaat
     2221 ccggcttgtc ggagttggcc ggcgtatcgt cgtacagctt gttgcggttg aggttggtca
     2281 gctggcggaa atctttgagg taaccttcca gcgtgaattc gagatttccc catttttcga
     2341 tcgtatcgat gggatcgtat tcgaacccga aaatgtaatg attcgctttc tgcagcttgg
     2401 acgtgatctg gtacgtgttc ccgttctggt cggtgaactg cgtgggaatg ttatccgatc
     2461 cggaaaggaa cccgtagaac aggttcacca cgtcacggtc ggaaacagcg ctgaggaggt
     2521 tctgcgaata cattcccgtt gcgcctttga tccggaagtg gttgctgaca ttatacttga
     2581 atgtaaggcg cggttcgggt gaagcattgt tcagggaagc gtaatactgg aaacggaaac
     2641 cgggttccac cacaagcgta tggttggcca gcgaatcttt cgccatgttc ttcgaaatcc
     2701 atttgtattt gaaatacgcg gcgaattccg tggtgttgtc gatctgggat atttccctgt
     2761 tcaccgggtt tatgaaatcg aagtctgttt tgaagccgag gatttcgaga ccgtaattga
     2821 ccacgttctt gcgctggaaa tacgtaaagt tcaggcccat gttgaaaccg tccacctcgc
     2881 tgtggcggcg gttggtgtaa tcttccgaca tcccgatgcc gtacctggaa tacgcgaaat
     2941 ttcccttgat gatcgtgttc gatccgccgg ggatcacctg gaagttgctc ccgaaaccaa
     3001 ccgatttcca ggaaaggtcg accgagttct tgtaatgatc cacgctgtcg ttgaacatga
     3061 ag
//
LOCUS       DASTSF010000103         6274 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1282169,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000103 DASTSF010000000
VERSION     DASTSF010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 6274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6274
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1282169"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(123..1136)
                     /gene="metX"
                     /locus_tag="VFU15_03100"
     CDS             complement(123..1136)
                     /gene="metX"
                     /locus_tag="VFU15_03100"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020598117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004414 - homoserine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HEU4716788.1"
                     /translation="MSIGKFIYDKTFATESGKTLPLLEIAYCTYGKFVPGKSKVVWVC
                     HALTASADCVSWWPGLIGNGKAIDPDEYFIVCANIPSSCYGSTGPLSVNPATGNPWYG
                     DFPVFSIRDIVTALIILRKHLGIGAIHLLIGGSMGGYQCLEWALMEKDVVENLCLVAT
                     GARESAWGIAIHTAQRLAIEADPTFGERKNYAGISGLRAARAMGMVTYRSYSAYVHTQ
                     TDEDHRTDDFKAESYIRYQGEKLVKRFNAYSYWLLTKTMDTHNVARGRGKMEDVLKTV
                     KAKTLCIGISSDFLCPVPEQKFMAAHIPGAKFEEIDSPFGHDGFLVEGKKIGEIVTGF
                     LRA"
     gene            complement(1192..1680)
                     /locus_tag="VFU15_03105"
     CDS             complement(1192..1680)
                     /locus_tag="VFU15_03105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716789.1"
                     /translation="MKNVIGVFAASLLLILSSCGGKPANPLVGKWKFEKMEMAKTDMT
                     SNMRKSMDTMMKDSGMADAMDTMAKSMNEMGNAMGDLGAGLASAFMTGSTYEFENDGT
                     LEISVLFTTSKGKYTLSSDNKSLTMTSDGKDQQFTVTKCDDQNLDLTTADNMVWHFTK
                     DK"
     gene            1817..2245
                     /locus_tag="VFU15_03110"
     CDS             1817..2245
                     /locus_tag="VFU15_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEU4716790.1"
                     /translation="MKVTLHRLNNAFHFEAKNEDGVTMQLDSTADHGGQNLGVRPMQS
                     LLMAMGGCSAIDIISILNKQKQEISGFDIDVDGEREKGKEPALWKTVHAIFRLNGNID
                     PEKAKRAVQLSMEKYCSVAKTLEAAGAVITYDVILNGQNL"
     gene            2325..3491
                     /locus_tag="VFU15_03115"
     CDS             2325..3491
                     /locus_tag="VFU15_03115"
                     /EC_number="4.2.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA];
                     GO_process: GO:0071268 - homocysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-succinylhomoserine sulfhydrylase"
                     /protein_id="HEU4716791.1"
                     /translation="MKPETSAIRIQNERTQEHEHSVPMYLTSSFVFDDAEHMRATFAD
                     ENDDNIYSRFTNPNVTEFVQKLCALEGTEDGFATASGMSAVFASFLAFLKSGDHLLAC
                     SSIFGSTHTVITKYLPKWGIGYSYADITKPGTWEALIKPETKMIFVETPTNPGLDIVD
                     LEWLGKLAAKHNVLLNVDNCFATPVLQQPAKYGAHLITHSATKFIDGQGRVLGGAVLG
                     KKELIREIYLLCRSTGPALSPFNAWVLSKSLETLHVRMERHCQNAQKLAGLLQHHPEV
                     SMVKYPFLLSHPHYEIARKQMSAGGGVVTFELKGGLEAGRKFLDKIKMCSLTANLGDT
                     RTTITHPASTTHAKLSEAERLAVGITPGLVRVSAGLEHITDIITDIEQALQQLK"
     gene            3545..>6274
                     /locus_tag="VFU15_03120"
     CDS             3545..>6274
                     /locus_tag="VFU15_03120"
                     /inference="COORDINATES: protein motif:HMM:NF019136.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triple tyrosine motif-containing protein"
                     /protein_id="HEU4716792.1"
                     /translation="MAAQESGYLIHNYSPKDYNGFNQVWCGVQDRLGILHFGTSGSII
                     SFNGQSWEKTPVRSGVAVRSMVIDTTSNIIYVGTVGDFGFMSTGPDGRMKFISLLDKI
                     PEADRVFADVWRTFLWKGGIVFQTEKSIFIYRNGKFTVIRARTSFALSFCVDGRLFIK
                     HREIGLSEVQGDSLHLLPGGELFSSLPLTGILKWNDGMKILLTGEEGIYTMDERNPRN
                     YFSPRPVSTDSFLLNAGILGGRWMGDSVLCVNSRTGIAFYNRNLRLKEQLTVRDGLAE
                     QSVADAFTDKENNLWLCTMNGITCISYGAPDRYYGKAAGFDGVFNCACSLNGILYIGT
                     NTGIYREVPSGIPGKRSFRLVRMRKTEVWRMRVVGNDLLAATSEGLLQVKDDKVIYLT
                     NAYANDISAYGSTEILVAEKNGLHYLKKDAAGNWQQQRYFEYDSREFINTSDLADVPG
                     KPGEREVWATTRMKEICRIRFDASDSLISMRTFVPDSNFICPYNIFPFNWGDSVYFWG
                     EYMALRYIPHLDDGGKGLCFGLADDLYNFHFPPASPGKNTETDVTGLFTSLPGSHTMR
                     VAWQAGNEMRSYITYISDAVGDDISSVYPAGNGELWFCGTDAMICYNTKTPVSSSASF
                     TALISRAIIGDSARYTGPGADQLSCTDKIPFRNNRFVFAFAAPFYNHGQETEFSYQLS
                     GYDTSWSEWTGKTEIQYNNLGEGDYVFRVKARNIFGDVSRVAEYHFTILAPWYRTTWA
                     YSLYVLSFAGLFFGSVRLSARRLRKQKEKLEKIVGERTAEVVQQKQQIEIQKSELETA
                     YTDIQDSIRYAERIQQAILPQQSEFSKYLRDSFVLFHPRDIVSGDFYWFAHRDGKSFI
                     ACVDCTGHGVPGAFMSMIGNTMLNQIVLEKKIDDPAMILQALHENVRQALKQNT"
BASE COUNT         1472 a         1731 c         1644 g         1427 t
ORIGIN      
        1 gcaaccgatg tcgagataaa ttccatccgc gtgcggggca agatgcgcaa agatcctccc
       61 ggcgagatag ggatccgcct gccgcgtgag gttgtaaccc gtgccgatcc tgtcgtaaag
      121 tgtcatgcgc gcagaaatcc ggtgacgatc tccccgatct tttttccctc caccaggaag
      181 ccatcatgac cgaacggtga atcgatctct tcgaacttcg cgccgggaat atgcgccgcc
      241 ataaatttct gctcgggaac agggcaaagg aaatcgctgc tgatgccgat gcacagcgtt
      301 ttcgccttca ctgttttcag cacatcttcc attttcccgc gaccgcgcgc cacgttgtgc
      361 gtatccattg ttttcgtcag cagccagtag gaataggcat tgaaccgctt caccagcttt
      421 tcaccctggt agcggatata cgattccgct ttgaaatcgt ctgtgcggtg atcttcatcc
      481 gtctgcgtgt gaacgtacgc ggagtaactg cggtaggtga ccattcccat tgcacgcgcc
      541 gcgcgaagcc cgctgatgcc ggcgtaattt tttctttcgc cgaacgtagg atccgcttca
      601 atggcgagac gctgcgcagt atgaatcgca attccccacg cgctttcccg cgcaccggta
      661 gcgacaagac acaggttctc cacaacgtcc ttttccatga gcgcccattc gaggcactgg
      721 taaccgccca tcgatccgcc gatgagcagg tggatcgcgc cgatcccgag gtgctttcgt
      781 aagataataa gcgcggttac tatatcgcgg atggaaaaaa cggggaagtc gccgtaccac
      841 ggatttcctg ttgccggatt gacgctcagc ggtcccgtgc tgccgtagca ggaagaagga
      901 atgttcgcgc agacgatgaa atattcatcc ggatcgatcg cttttccatt cccgataaga
      961 ccgggccacc aggaaacgca gtcggcgctg gcggtgagcg catggcacac ccataccact
     1021 ttgcttttcc ccggaacgaa ttttccgtag gtgcagtaag cgatctccag caacgggagc
     1081 gttttgccgc tttccgtcgc gaacgttttg tcgtaaatga attttccgat gctcataaaa
     1141 aaacccgcag ctgttacggc cgcgggtcaa agctactgaa accgtaactg attatttgtc
     1201 tttggtgaaa tgccacacca tgttatccgc tgtcgtcagg tcgaggttct gatcgtcaca
     1261 cttggtcacg gtgaactgct ggtctttccc gtcgctcgtc atcgtcagcg atttattgtc
     1321 ggacgaaagc gtatattttc ctttggacgt cgtgaaaagc acgctgatct cgagcgttcc
     1381 gtcgttctcg aattcatacg tagaaccagt catgaaagcg ctggcgagac ctgcacccag
     1441 gtcgcccatg gcattgccca tttcattcat gcttttggcc attgtatcca tcgcgtcggc
     1501 catgccggaa tccttcatca tcgtatccat gcttttccgc atgttgctgg tcatgtccgt
     1561 tttagccatt tccatttttt cgaatttcca ttttccaacg agcggatttg caggttttcc
     1621 tccgcaggac gaaagaataa gcaggaggga tgcggcgaaa acgccaatta catttttcat
     1681 ggtgaataga gtttaggttt tgtcccgcaa aagtagtcca atttgagaga cgggaaaata
     1741 ttcccccggt tttgtgcagg accgttttcc attcgggaaa ggcgctttcc ttatctttga
     1801 tgctcacaga aaaaagatga aagtcacttt acacaggctt aataacgctt tccatttcga
     1861 agcaaagaat gaagacggcg ttaccatgca gctcgacagc accgcagatc atggcggaca
     1921 gaatctcggt gttcgcccga tgcaatccct gctcatggct atgggcggat gcagcgcgat
     1981 cgacatcatt tccatcctga acaagcagaa gcaggaaatc tccgggttcg atatcgacgt
     2041 cgacggtgag cgcgaaaaag gaaaagagcc tgcattgtgg aaaacagttc atgccatctt
     2101 caggctgaac gggaacatcg atcccgaaaa agcaaaacgc gccgtgcagc tttcgatgga
     2161 aaaatactgt tccgtggcga aaacgctgga agcggcgggt gcggtgatca cgtacgacgt
     2221 cattctcaac ggccagaatc tttaagcaat ccccggtttc ctgttttttc atccgttcag
     2281 aatcgtaaaa ccggcaaccc gtaaccgaaa accgagcacg cgtcatgaaa cccgaaacgt
     2341 ccgccatacg catccagaac gaacgaacgc aggaacacga acattccgtt ccgatgtacc
     2401 ttacctcgag cttcgtgttc gacgatgcgg aacacatgcg cgccacgttc gccgacgaga
     2461 atgatgacaa tatttacagc cgttttacga atcccaacgt gaccgaattc gtgcagaagc
     2521 tctgcgcgct cgaaggaaca gaagatggtt tcgccacggc atcgggaatg tcggcggtgt
     2581 tcgcttcatt tctcgctttc ctgaaaagcg gcgatcacct gcttgcgtgc agctccattt
     2641 tcggatcgac gcataccgtg atcacgaaat atcttccgaa atggggcatc ggctattcgt
     2701 atgccgacat cacgaaaccc ggaacatggg aggcgctgat aaagccggaa acgaaaatga
     2761 tcttcgtgga aacgccaact aaccccgggc ttgacatcgt cgatctcgaa tggctcggca
     2821 agctggccgc gaagcacaac gtactgctca acgtcgacaa ctgttttgca acgcccgttc
     2881 tccagcagcc ggcgaaatac ggtgcgcatc tcatcacgca ttccgccacg aaattcatcg
     2941 acgggcaggg acgcgtgctt ggcggagcgg tgctcgggaa aaaagagctg atcagggaaa
     3001 tttatcttct ctgccgcagc acaggtccgg cgctttcacc gttcaatgcg tgggtgcttt
     3061 caaaaagcct cgaaacgctc cacgtgcgca tggagcgtca ttgccagaat gcgcagaagc
     3121 tggcgggcct gctgcagcat cacccggaag tttcaatggt aaaatacccg ttcctgcttt
     3181 cgcacccgca ctacgagatt gcgcggaagc agatgagcgc aggcggaggc gtggtgacgt
     3241 tcgagctgaa aggcgggctc gaagccggaa gaaaattcct ggataaaata aaaatgtgct
     3301 cgctcaccgc gaatctcggc gacacgcgca cgacgatcac gcatcccgcc tccaccacgc
     3361 acgcgaagct ttccgaagcg gaacgtcttg ccgtgggaat tacgcccggg ctcgttcgcg
     3421 tttccgcggg gctcgagcac atcaccgata tcatcaccga catcgaacag gcgctgcaac
     3481 agctgaaatg aaaaacaggc tcagggcact tttgttttta tgcggatgtt ttttcattcg
     3541 cccggtggcc gcgcaggaat ccggttacct cattcataat tattccccga aagactacaa
     3601 cggcttcaac caggtgtggt gcggcgtgca ggatcgtctc ggcattctcc atttcggcac
     3661 aagcggaagt atcatttctt tcaacgggca gagctgggaa aaaacgcccg tgcgttcagg
     3721 agtcgccgtg cgcagcatgg tgatcgacac cacctccaat attatttacg tcggtacagt
     3781 cggcgatttc ggtttcatga gcacgggtcc tgacgggcgc atgaaattca tctcgctgct
     3841 cgacaagatc ccggaagcgg accgtgtgtt cgccgatgtg tggcgaacgt tcctctggaa
     3901 aggcggcatc gttttccaga ctgaaaaaag cattttcatt taccgcaacg ggaaattcac
     3961 cgtcatccgt gcacgaacat cctttgcgct ttcgttctgc gtcgacgggc gccttttcat
     4021 caagcacagg gagatcggtc tttcggaagt gcagggcgat tcgcttcacc ttctcccggg
     4081 cggagaattg ttttcgtccc tgccgctcac cggcattctc aaatggaacg acgggatgaa
     4141 aattctcctg acgggcgaag aaggaattta caccatggac gaacgcaatc cccggaatta
     4201 tttttccccg cgacctgtca gcacggattc gttccttctc aacgccggca ttctcggcgg
     4261 gcgatggatg ggcgactccg ttctttgcgt caattcgcgc accgggatcg cattctacaa
     4321 caggaacctc cggctgaaag agcagctcac cgtgcgcgat ggactcgccg agcagtcggt
     4381 agccgacgca ttcaccgaca aggaaaacaa tctttggctg tgcacgatga acggcatcac
     4441 ctgcatttcc tatggcgctc ccgaccgtta ctacgggaaa gcagcaggct tcgacggcgt
     4501 gttcaactgc gcgtgctcgc tgaacggcat tctctacata ggcaccaaca cggggatcta
     4561 ccgcgaagtt ccatcgggta ttccgggcaa acgttctttc cgcctggtcc ggatgcgcaa
     4621 aaccgaagtg tggcgcatgc gcgttgtggg aaacgacctg ctggcggcta cttcggaagg
     4681 acttctccag gtaaaggacg ataaggtgat ttacctgacg aatgcttatg caaatgatat
     4741 ttccgcctat ggatccacgg aaatcctcgt cgccgaaaaa aacggcctgc attacctgaa
     4801 gaaagatgcg gccggcaact ggcagcagca gcgttatttc gaatacgaca gccgcgagtt
     4861 catcaacacc tccgacctgg ccgacgtgcc gggcaaaccc ggtgaacgtg aagtatgggc
     4921 aaccacgcga atgaaagaga tctgccgcat acgtttcgat gcttcggatt ccctcatcag
     4981 catgcggaca ttcgttccgg acagcaactt catctgcccg tataacattt ttccgttcaa
     5041 ctggggcgac agcgtttact tctggggtga atacatggcg ctgcgttaca ttcctcacct
     5101 cgatgacgga ggaaaaggcc tctgcttcgg tctcgccgat gatctttaca attttcattt
     5161 tccgcctgcg agtccgggaa aaaacacgga aacggatgtc acgggtctct tcacttcatt
     5221 acccggaagt cacaccatgc gcgtggcgtg gcaggcgggc aacgagatga gatcgtacat
     5281 tacgtatatc tcggatgctg tcggagatga tatttcatcg gtttacccgg ccggtaacgg
     5341 agagctgtgg ttctgcggaa cggatgcaat gatctgctac aacacgaaaa cgcctgtttc
     5401 ttcttccgct tcgttcaccg cgctgatatc acgggcgata atcggcgatt cagcccggta
     5461 cacgggaccc ggcgcggacc agctttcgtg cacggataaa attccgttcc ggaacaaccg
     5521 tttcgtattt gcgttcgcag cgccgtttta caatcacggg caggaaaccg aattttccta
     5581 ccagctttcc ggttatgaca ccagctggtc ggaatggacc ggcaaaacag aaatacagta
     5641 caacaacctg ggcgaaggcg attatgtttt ccgtgtaaag gcgcggaata ttttcggtga
     5701 tgtcagccgt gtggccgaat atcattttac gatcctggcg ccgtggtacc ggacaacctg
     5761 ggcttattcc ctgtatgtgc tttctttcgc cggccttttc ttcggatcgg tacggctgag
     5821 tgcacgacgt ctgcggaagc aaaaggaaaa gctggaaaag atcgtgggag aaagaacggc
     5881 ggaagtggtt cagcaaaaac agcagatcga gatccagaag agcgagctcg aaaccgcgta
     5941 caccgacata caggacagta ttcgttacgc cgaacgcatc cagcaggcga tccttccgca
     6001 acagtccgaa ttttcaaaat acctgcgcga ttctttcgtg ctcttccacc cgcgcgatat
     6061 cgtcagcggc gatttctact ggttcgccca ccgcgacgga aaatcattca ttgcctgcgt
     6121 ggactgcaca ggtcacggcg ttccgggcgc gttcatgagc atgatcggca acacgatgct
     6181 gaaccagatc gtgcttgaga aaaagatcga cgaccccgcc atgatcctgc aggcgctgca
     6241 tgaaaacgtg cgccaggctt tgaagcagaa tacc
//
LOCUS       DASTSF010000104         6327 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1497248,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000104 DASTSF010000000
VERSION     DASTSF010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 6327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6327
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1497248"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..550)
                     /locus_tag="VFU15_03125"
     CDS             complement(<1..550)
                     /locus_tag="VFU15_03125"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013616618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEU4716793.1"
                     /translation="MKFEIIHPDDKSNARAGKITTAHGEIETPIFMPVGTAGTVKAIH
                     MRELENDIGAQIILGNTYHLFLRPGLDVLEHAGGLHKFNGWNRSILTDSGGFQVYSLS
                     HRRKLTEDGVHFSSHIDGSKHLFTPENVMDIQRTIGADIIMAFDECTAYPCEYADAKK
                     SMEMTHRWLKRCTARFDSTESKY"
     gene            696..1832
                     /locus_tag="VFU15_03130"
     CDS             696..1832
                     /locus_tag="VFU15_03130"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU4716794.1"
                     /translation="MTLQQFTTLSPGMIAFWVLAFAVLVQLFYHFYFFLRVATYRKKE
                     KPAGELPPVSVVICARNEDDNLVSYLPLVLAQDYSDFEVVVVNDCSYDNTGDILEEFA
                     KKNAKLKVVTIKEDEYYQHGKKVAVMMGIKGAKHEHLVLTDADCRPATDQWLKSMAGH
                     FHGGKEIVIGYGPYERGKGFLSKLIRFDSFFIGLQYLSYALARVTYMGVGRNLAYTKK
                     LFYSVKGFASHYHIRSGDDDLFVNEAATRSNVSVELGKETFCYSPAKKKFRDWWYQKK
                     RHLETGKRYRFSHRFLLGLYVMSQWLFFAAFITALVLRFNIYAVCGAFLIRFCVQMLI
                     FNSSMKKMEERDLLVLAPLFELFFLLFYPVITVSRIFQRKNRWN"
     gene            1823..2437
                     /locus_tag="VFU15_03135"
     CDS             1823..2437
                     /locus_tag="VFU15_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014680539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU4716795.1"
                     /translation="MELNENENLSEKALYDYRLVKRALEDADQKAFAELMGRYRDSVY
                     YMLLKMVNNKDDAEDLTIEAFGKAFKRLSQYTPNFAFSTWLFRIATNNCIDFIRRKRK
                     NTFSIDQPMEDDEGGEMVLDLRSETPDPEEHIIKKQKVILLRELVEKLKPRYRTLIEM
                     RYFQELSYEEIAEQLELPLGTVKAQLFRAREFLFNVLKNTQGKI"
     gene            2474..3127
                     /gene="rsmG"
                     /locus_tag="VFU15_03140"
     CDS             2474..3127
                     /gene="rsmG"
                     /locus_tag="VFU15_03140"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008198758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEU4716796.1"
                     /translation="MTLANQHTGTLTAVSRYFTDLSTNQQAKFSMLGELYEHWNAQIN
                     VISRKDMEQFYLHHVLHSLAIAKVIRFKAGTEVLDAGTGGGFPGIPLAIFFPEVKFHL
                     VDSIGKKIKVVNEVAHALDLKNVTAQQVRAEELGMQYDFITSRAVAQWSDLHRWTAKL
                     LKKKQQNALPNGWLLLKGGDLKDEFYSVRKKIVEYPVSDFFSEDYFETKKVIYVPAG"
     gene            complement(3343..5199)
                     /locus_tag="VFU15_03145"
     CDS             complement(3343..5199)
                     /locus_tag="VFU15_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HEU4716797.1"
                     /translation="MPKARVSRESLKRSARLFSYMKGHRWKFALGLVFLGLTATTALI
                     FPRMMGELMGIIGGTSGTGSLPSMDKLTHPDNSFLSADVKARLIHAANTVGMQLIILF
                     SLQSVFSFFRVYMFSQATENMLARLRQDVFSRTLRTSMTFFSRNQAAELSSRISNDIS
                     QIGETMTTGVAEFLRQFIIIVGGITIICFISWKLALVMLAIVPPVAFVTVFFGRRIRK
                     HSRLVQDKIAESNVIVTESFQGITNVKSFTNEGYEIGRYTKSTKEIVQQAIRYAISRG
                     SFFSFIIFCLFGSMMLMVWFGVRMTVLDELTAGQMLSFLFYTVFISASFGGISEQYAQ
                     VQRAMGASDRVFEILAEETEKVKTDDERKRNVLRLRGEVEFIHTEFSYPTRKEFPVLR
                     DISFRANPGETIAIVGPSGSGKSTIAQMLLRFYDPDKGQILFDGKEAGTYDLTELREN
                     MAIVPQDVILFGGTIRENIVYGKPGATENEIREAALKANALSFIESFPDKFETKVGDR
                     GIQLSGGQRQRIAIARAVLKDPSILILDEATSSLDSESERVVQEALDKLMVGRTSFVI
                     AHRLSTIRDADRIIVIEKGNVVEAGTHEELMAIENGLYRSLNRLQFQGHEVM"
     gene            5474..5629
                     /gene="rpmH"
                     /locus_tag="VFU15_03150"
     CDS             5474..5629
                     /gene="rpmH"
                     /locus_tag="VFU15_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015029776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEU4716798.1"
                     /translation="MKRTFQPSKRKRKNKHGFRERMSTANGRRVLAARRKRGRKKLTV
                     SSEKTHK"
     gene            complement(5695..>6327)
                     /locus_tag="VFU15_03155"
     CDS             complement(5695..>6327)
                     /locus_tag="VFU15_03155"
                     /inference="COORDINATES: protein motif:HMM:NF025580.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4328 domain-containing protein"
                     /protein_id="HEU4716799.1"
                     /translation="LLIRFVFVAQDSIGSFQLVRMAIIGNGIIGLVQTVAYILSIIFF
                     IMWFRRAYNNLHLMGSQFLTFSEGWAAGAWFVPFMNLVRPFRIMREIWNETQMLGRNP
                     SELFTVEEPDITAWWWGTWLGGNIVSSIHPNGSSMLDPGYDASDSIRIIISFALLIIS
                     GLILVRIIRKISAWEDELMIRYNNHEALRQQRAYTEKSQQNPYGQQPPQV"
BASE COUNT         1489 a         1630 c         1738 g         1470 t
ORIGIN      
        1 catatttgct ttccgtggaa tcgaagcgtg cggtgcagcg tttcagccag cggtgcgtca
       61 tctccatcga ctttttcgca tccgcatatt cgcagggata cgcggtgcat tcatcgaacg
      121 ccataatgat atccgcgccg atcgtgcgct gaatgtccat cacgttttca ggcgtgaaga
      181 ggtgcttcga cccgtcgata tgactggaaa aatgaacacc gtcttccgtg agcttgcggc
      241 ggtgcgaaag cgaatacacc tggaagccgc cgctgtcggt gagaatggac cggttccagc
      301 cgttgaattt gtgcagcccg cccgcgtgtt caagaacatc aagacccggg cggagaaaaa
      361 gatggtaggt gttcccgaga atgatctgcg cgccgatgtc attttccagc tcgcgcatgt
      421 ggatcgcttt caccgttcct gctgttccca cgggcatgaa gatcggcgtt tcgatttcgc
      481 cgtgtgccgt ggtgatcttt cccgctcttg cgttgctttt gtcgtccgga tgaatgatct
      541 cgaatttcac cggggcaaaa gtacggcttc gccttcaatc gcggaggatc gcgttgtcag
      601 ggcaaaatgc atcgcggaat gttaattact ttgttgaata ccttgaccca ttcttaaaat
      661 gcctccggaa gcacaggttt acatttgttt ccggcatgac gcttcagcaa ttcactaccc
      721 tttcgcccgg catgatcgca ttctgggtgc tcgcgttcgc cgtgctggtg cagctgttct
      781 accattttta tttttttctc cgcgttgcga cgtacaggaa aaaagaaaaa cccgccgggg
      841 aacttccgcc tgtttccgtc gtcatctgcg cccggaatga agatgacaac ctcgtcagct
      901 acctgccgct cgtgctggcc caggattatt ctgatttcga agtcgtcgtc gttaatgatt
      961 gctcgtacga caacaccggc gacattctcg aagagttcgc gaagaagaat gcgaagctga
     1021 aggtcgttac cataaaagaa gacgaatact accagcacgg gaaaaaagtg gcggtgatga
     1081 tgggcatcaa aggagcgaag cacgagcatc ttgttctcac cgacgccgat tgccgtccgg
     1141 ccaccgatca atggctgaaa agcatggcgg gacattttca cggcgggaag gagatcgtca
     1201 tcggttacgg accgtacgaa cgcgggaaag gatttctcag caagctcatc cgtttcgatt
     1261 cgttcttcat cgggctgcaa tatctttctt acgcgcttgc gcgcgtgacg tacatgggcg
     1321 tgggaagaaa cctcgcgtac accaaaaaac ttttttacag cgtgaaaggt ttcgcgtcgc
     1381 attaccacat ccgttccggt gacgacgatc ttttcgtgaa cgaggcggcc acgcgttcga
     1441 atgtgagcgt ggagctgggg aaggaaacgt tctgctattc gcccgcgaaa aaaaagttcc
     1501 gcgactggtg gtaccagaaa aaacggcacc tcgaaacagg aaagcgttac cgtttttcac
     1561 accgtttcct gctcggcctg tacgtgatga gccagtggct gttcttcgcc gcgttcatca
     1621 cggcgctggt tttacgtttt aacatctacg cggtgtgcgg cgcatttttg atccgcttct
     1681 gcgttcaaat gcttatcttt aattcctcga tgaaaaagat ggaagagcgg gacctcctgg
     1741 tgctcgcgcc tttgtttgag ctatttttcc ttctttttta ccctgtgata actgtctcca
     1801 gaattttcca gcggaagaac agatggaact gaacgaaaac gaaaacctgt cggaaaaagc
     1861 cctgtacgat tacaggctgg tgaagcgtgc gctggaagat gccgaccaga aagcgtttgc
     1921 cgagctgatg ggacgttacc gtgattccgt ttattacatg ctgctgaaga tggtgaacaa
     1981 caaggacgat gcggaggatc tcacgatcga ggcgttcgga aaagcgttca agcgcctttc
     2041 gcagtatacg ccgaacttcg ccttcagcac ctggctgttc cgcattgcga caaacaactg
     2101 catcgacttt atccgccgca agcgcaagaa tacgttctcg atcgaccagc cgatggaaga
     2161 tgacgaaggc ggggaaatgg tgctcgacct tcgttccgaa acacccgatc ccgaagagca
     2221 cattatcaaa aagcagaagg ttattcttct gcgcgagctc gttgaaaaac tgaagccgcg
     2281 ctaccgcacg cttatcgaga tgcggtattt ccaggagctg tcgtacgagg agatcgccga
     2341 gcagcttgaa ctgcctttgg gaacggtgaa agcgcaactg ttccgtgcac gggaattcct
     2401 cttcaacgtg ctgaaaaaca cccagggcaa gatctgatgt gctgacacat ccttgtcctc
     2461 aaattatccg atcatgacat tggccaacca gcacacgggt acgttgacag ccgtctcccg
     2521 ttattttacc gatctctcca ccaaccagca ggcgaaattt tccatgctgg gtgaattgta
     2581 cgagcactgg aatgcgcaga tcaacgtcat ttcgcggaag gacatggagc agttttacct
     2641 gcatcacgtg ctgcacagcc tggcgatcgc gaaggtaatc cgtttcaagg caggtacgga
     2701 agttctcgac gcaggaacgg gcggcgggtt tcccggcatc ccgctcgcga tctttttccc
     2761 ggaagtgaaa ttccatctcg tcgattccat cgggaaaaaa ataaaagtgg tgaacgaggt
     2821 cgcgcatgcg ctcgacctga agaacgtcac cgcgcagcag gtgcgggcgg aagagctggg
     2881 gatgcagtac gactttatca cttcgcgcgc tgtcgcgcag tggagcgacc ttcaccggtg
     2941 gacggcaaag ctgctcaaaa agaaacagca gaacgcactg ccgaacgggt ggctgttgtt
     3001 gaaaggcggc gaccttaaag atgaatttta cagcgttcgc aaaaagatcg tggaatatcc
     3061 tgtcagcgac tttttcagcg aggattattt cgaaacgaag aaagtgatct acgtgccggc
     3121 agggtgaagg aaaatgtcag cccgcaggac aggaagaagt ttctcaattc aattactgtt
     3181 acgattaccg gaccggcacc cggttacgcc ggatgatctc cccgtgcacg gttaccgggc
     3241 gcctgtgcgt gaaattgccg caatgctcgc gcagccgtgg atcgacccca aaggcccgga
     3301 gttcagcgtt tgcgttgcat ccggaatccg gaacggaaat gctcacatca cttcgtgtcc
     3361 ctggaactgc aggcggttga ggctgcggta aagcccgttc tcgattgcca taagctcttc
     3421 gtgcgtgccg gcttccacca cgttcccttt ttcgatgacg atgatgcggt cggcgtcacg
     3481 gatcgtcgag aggcggtgcg cgatcacgaa tgaagttctt cccaccatca gcttgtcgag
     3541 cgcttcctgc accacgcgtt cgctttcgga atcgagggag ctggtggctt cgtcgaggat
     3601 gaggatggaa ggatctttca gcaccgcgcg tgcgatggcg atacgctggc gctgtccgcc
     3661 ggaaagctgt atgccgcggt cgccgacttt cgtttcaaat ttatcaggga agctttcaat
     3721 gaaggaaagc gcattggctt tcagggccgc ttcgcggatt tcgttctccg tcgcgccggg
     3781 ttttccgtac acgatattct cacggatcgt tccgccgaag agaatgacgt cctgcggaac
     3841 gatggccatg ttctcgcgca gttcggtgag atcgtacgtg ccggcttctt tcccgtcaaa
     3901 taagatctgt cctttgtccg gatcgtaaaa acgcagcagc atctgcgcaa tggtagattt
     3961 gcctgaaccg gaaggaccga caatggcgat cgtttcgccg ggattcgccc ggaagctgat
     4021 gtcgcgcagc acgggaaact cttttcgcgt cgggtaagaa aattccgtgt gaatgaattc
     4081 cacttctccg cggaggcgaa gcacgttgcg tttccgctcg tcgtccgttt ttaccttttc
     4141 cgtttcctca gcgagtattt cgaacacacg gtcggaagcg cccatggcgc gctgcacctg
     4201 tgcatactgt tcggaaattc cgccgaagga agccgaaatg aaaacggtgt agaaaaggaa
     4261 ggaaagcatt tgcccggccg tgagctcatc gaggaccgtc attcgcacgc cgaaccaaac
     4321 catcagcatc atcgaaccga aaaggcagaa aatgatgaac gagaaaaatg atccgcgcga
     4381 gatggcgtaa cggatcgcct gctgcacgat ctctttcgtg cttttcgtgt aacgcccgat
     4441 ctcgtaacct tcgttcgtga atgattttac gttcgtgata ccctggaagc tttcggtgac
     4501 gatcacgttc gattcggcaa ttttatcctg cacaaggcgg gagtgtttcc ggatccttct
     4561 tccgaaaaac acggtgacga atgcgaccgg cggaacaatg gcgagcatta cgagcgccag
     4621 cttccagctg atgaagcaga tgatcgtgat accgccgacg ataatgatga actggcgtag
     4681 aaattccgcc acgccggtgg tcattgtttc accgatctgc gaaatgtcgt tgctgatgcg
     4741 gctgctgagc tccgccgcct ggtttctcga gaagaacgtc atggacgtgc gcagcgtgcg
     4801 tgagaaaaca tcctggcgca gccgggcgag catattttcc gtcgcctgcg agaacatgta
     4861 tacgcggaag aatgaaaaaa cggactgcag cgaaaaaagg atgatgagct gcatgcccac
     4921 ggtgttcgcg gcatggatga gacgtgcttt tacatcagcg gaaaggaatg agttgtccgg
     4981 gtgcgtgagc ttgtccatgg aaggaaggct ccccgttccc gacgttccgc cgataatgcc
     5041 catgagttcg cccatcatcc gcgggaagat gagcgccgtg gttgcggtga gcccgaggaa
     5101 aacgagtccc agcgcaaatt tccagcgatg tcccttcatg taagagaaaa gccgtgccga
     5161 gcgtttcagg ctttcgcgcg aaacccgtgc cttgggcatg tcggtgtctt tgggttcggt
     5221 tctcagtcct cgtctcggtc ctgccatacg tctgcttcag ttagaaatta gagcgcaaaa
     5281 ttacccaaaa acgaaaggcg ttccgggtta atgtccctgt gaaaagtcgc gcagcacccg
     5341 gatttatttt acccggggcg gggcaggttg tattaaaggt tctgatcatc agccatttaa
     5401 attcatttca aggttgattt gcatgaaaag ccaatatctt tatctttgca gtcccaattc
     5461 aaacagtaaa gcgatgaaac gtacattcca gccttcgaag cgcaagagaa aaaacaagca
     5521 cgggttccgt gagcgcatgt caactgcaaa cggccgccgt gtgctggctg cacgtcgtaa
     5581 gcgcggacgc aagaagctta cggtttcgag cgaaaagacg cacaaataat ccgttttcat
     5641 acaagaacga aaggcgctga taaggcgcct tttttgtttt gcagtgaacg aagatcatac
     5701 ctgcggcggt tgctgcccgt aagggttttg ctgcgacttt tccgtgtacg cccgctgttg
     5761 acgcagtgcc tcatggttgt tgtaacggat catgagctca tcttcccacg cggaaatttt
     5821 acggatgatg cggacgagaa tgaggccgga aatgatcagc agggcgaatg aaatgattat
     5881 gcggatcgaa tcggaagcgt cataaccggg atcgagcatg ctgcttccgt tcggatgaat
     5941 gctggaaacg atattcccgc ccagccaggt accccaccac caggcggtga tatcgggttc
     6001 ttccaccgtg aataattccg acgggtttct tcccagcatt tgcgtttcgt tccagatctc
     6061 gcgcatgatc ctgaacgggc gaacgaggtt cataaagggc acgaaccatg cccccgcagc
     6121 ccagccttcg ctgaacgtca ggaattgcga tcccatgagg tgaagattat tataggcgcg
     6181 gcggaaccac ataatgaaga aaatgatgga caggatatag gcgaccgtct gtacaagacc
     6241 gatgatcccg ttaccgataa tagccatgcg tacgagctgg aaggatccga tggaatcctg
     6301 tgcgacaaaa acgaaacgga taagcag
//
LOCUS       DASTSF010000105         3286 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1943573,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000105 DASTSF010000000
VERSION     DASTSF010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3286
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1943573"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /gene="rplP"
                     /locus_tag="VFU15_03160"
     CDS             complement(<1..218)
                     /gene="rplP"
                     /locus_tag="VFU15_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019596872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEU4716800.1"
                     /translation="MLQPKRTKFRKQHKMKIKGKATRGHQISFGSFAIKTLEECWITA
                     KQIEAARVAMTRYLKREGQVWIRIFPDKP"
     gene            complement(253..1005)
                     /gene="rpsC"
                     /locus_tag="VFU15_03165"
     CDS             complement(253..1005)
                     /gene="rpsC"
                     /locus_tag="VFU15_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013633180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEU4716801.1"
                     /translation="MGQKANPIGNRLGIIRGWESNWYGGKNYSDKLVEDEKIRNYLKA
                     RLAKGSISKIVIERTMKLITVTIHTARPGIIIGKGGKEVDKLKEEIKKLTKKEVQINI
                     FEIKRPELDARLVADSIARQIEGRISFRRAIKMSIASTMRMGAEGIKVKCSGRLAGAE
                     IARVEGYMEGRVPLHTLRADVDYAVAEAHTSYGRIGVKVWICKGELYGKRDLSPNSAN
                     AVREKKSGPNQGGGARGGRQRRGGRKETGNQG"
     gene            complement(1027..1428)
                     /gene="rplV"
                     /locus_tag="VFU15_03170"
     CDS             complement(1027..1428)
                     /gene="rplV"
                     /locus_tag="VFU15_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEU4716802.1"
                     /translation="MARRKEHVANARKEEMKTTYIARLRNVPTSPRKMRLCADLIRGK
                     EVFKALNILRFTNKAPAERLEKLLRSAISNYEKKSGNTAQADELFVKTIMVDGATILK
                     RISAAPQGRAHRIHKRSNHVTLVIDEISNKA"
     gene            complement(1450..1728)
                     /gene="rpsS"
                     /locus_tag="VFU15_03175"
     CDS             complement(1450..1728)
                     /gene="rpsS"
                     /locus_tag="VFU15_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007212071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEU4716803.1"
                     /translation="MARSLKKGPFVDPKLDKKIVAMNAGGKKQAIKTWSRRSMITPDF
                     VGHTFAVHNGNKFIPVYVTENMVGHRLGEFAPTRTFRGHSGNKDKAST"
     gene            complement(1754..2578)
                     /gene="rplB"
                     /locus_tag="VFU15_03180"
     CDS             complement(1754..2578)
                     /gene="rplB"
                     /locus_tag="VFU15_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004291237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEU4716804.1"
                     /translation="MALKKFRPLTPAMRHRVIVTSSDITSATPEKSLLLPVKKSGGRN
                     SSGKMTMRYIGGGHKRMFRIIDMKRDKHGIEGVVKTVEYDPNRSARIALVVYKDGEKR
                     YIIAPNGLKVGQKVMSGKNAAPEVGNTLFLADVPLGTLVHNIEMRPGQGGKIARSAGS
                     YAQLSAREGRFAVLRLPSGETRMVLTTCLATIGSVSNSDHNLQSDGKAGRKRWLGRRP
                     RNRGVAMNPVDHPMGGGEGRASGGHPRSRKGLKAKGLKTRSLKKASNRYIIERRKK"
     gene            complement(2600..2890)
                     /gene="rplW"
                     /locus_tag="VFU15_03185"
     CDS             complement(2600..2890)
                     /gene="rplW"
                     /locus_tag="VFU15_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEU4716805.1"
                     /translation="MNVLIKPIITEKMSAQGEKFSRYGFVVDQRANKMQIKNAVEKMY
                     GVTVKTVNTQNYIGKVRSRFTRSGMQVGVANRQKKAVVTLKQGDVIDFYSNL"
     gene            complement(2894..>3286)
                     /gene="rplD"
                     /locus_tag="VFU15_03190"
     CDS             complement(2894..>3286)
                     /gene="rplD"
                     /locus_tag="VFU15_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEU4716806.1"
                     /translation="SPLFKGGGRVFGPEPRDYKIRLNKKVKALARKSALSYKAKENNI
                     LVLEDLSFDAPKTGMYNEILGKLNVADKKTLLVTGNYNKNVYLSSRNLERAKVVTAAE
                     LTTYDILNASNILLSESSVSTIENLLKN"
BASE COUNT          673 a          847 c          800 g          966 t
ORIGIN      
        1 ggcttatccg ggaaaatacg gatccacacc tgtccttcgc gcttgaggta acgtgtcatt
       61 gcaacacggg ccgcctcgat ctgcttggca gtgatccagc attcttcaag tgttttgatt
      121 gcgaaagagc caaatgaaat ctggtgaccg cgggtagctt tacccttgat cttcatcttg
      181 tgctgcttac ggaatttcgt tctttttggt tgtaacatga ccttctgtta ttaatcggtt
      241 ggccgttttc ggttatccct gatttccggt ttctttacgt ccgcctctgc gctgacgtcc
      301 gcctcttgcg ccgccgccct ggttcggacc ggattttttc tccctgaccg cattggcaga
      361 attcggggaa agatcacgct ttccgtaaag ttcacctttg caaatccaca ctttcacacc
      421 gatacgtccg tatgaagtgt gtgcttccgc cactgcgtag tcaacgtcag ctctcagagt
      481 atgcagcgga acgcgtccct ccatatagcc ttccaccctt gcgatttccg ctccggcgag
      541 ccggccggaa cattttacct tgatgccttc agctcccata cgcatggtag aagcgattga
      601 catcttgatt gcacgacgga aagaaatacg accttcgatc tggcgggcaa tgctgtctgc
      661 cacgagacgg gcatcgagtt cgggacgctt gatctcaaaa atattgatct gcacttcctt
      721 ctttgtcagc ttcttgatct cttctttcag cttatccact tccttacctc cctttccgat
      781 gatgatcccc ggacgggctg tatggatagt tacggtgata agcttcattg tgcgctcgat
      841 cacaattttt gaaatgcttc ctttggcgag gcgggccttc aggtaattgc ggattttttc
      901 atcctcgacc agcttgtcgg aataattctt tccgccgtac cagttggatt cccagccgcg
      961 gatgattccg agcctgttgc cgataggatt tgctttttgt cccattcttt tctttcgttg
     1021 tgaccgttat gctttattgg aaatttcgtc aataacaagc gtcacatggt tggagcgctt
     1081 gtggatcctg tgcgcgcggc cctgcggcgc ggcagaaata cgctttaaaa ttgttgcccc
     1141 gtcgaccatg atcgtcttta caaaaagctc gtcagcctgg gccgtatttc cggatttctt
     1201 ttcgtaattc gaaatagccg acctcagcag tttttccagg cgctctgcag gagctttatt
     1261 cgtaaagcgg agaatattca gtgctttgaa aacttctttt ccacgaatga gatcagcaca
     1321 gagacgcatt ttccggggag aagtaggaac attacgaaga cgggcgatat aggtcgtctt
     1381 catctcctct ttacgcgcgt ttgcaacgtg ttcttttcta cgtgccattg tttagtcttt
     1441 ttcttgttat caggtggaag ccttgtcttt gtttcctgag tgaccacgga aggtccgcgt
     1501 cggggcgaat tctcccaggc ggtgtccaac catattttcc gttacataaa cagggatgaa
     1561 tttattcccg ttatgcaccg cgaatgtatg cccgacaaaa tcaggtgtga tcatcgaacg
     1621 gcgagaccac gttttgatcg cctgcttctt accgcctgcg ttcatcgcga cgatcttttt
     1681 atcgagttta gggtctacaa acggaccttt tttcagtgag cgtgccattt attctctgta
     1741 ttacagctta gaattatttt ttacggcgtt cgatgatgta cctgttcgat gcctttttca
     1801 gtgaacgggt tttaagtcct ttcgccttga ggcccttacg tgaacgcggg tgaccgccgg
     1861 aagcacgacc ttcgccaccg cccatcgggt gatcgacagg gttcatggct acaccgcggt
     1921 tgcgcggacg acgaccgagc cagcgcttgc gtcctgcttt tccgtcgctc tggagattat
     1981 gatcagaatt ggaaacagaa ccgatcgtag cgaggcaggt ggtaagcacc attctcgttt
     2041 caccggaagg caagcgaaga accgcgaagc ggccttcacg cgcgctgagc tgggcatagg
     2101 agcctgcgct gcgggcgatc tttcctccct gtccgggacg catttcaata ttgtgcacaa
     2161 gcgtacccag cggcacgtca gcaaggaaaa gcgtattccc gacttcagga gcagcattct
     2221 ttcctgacat tactttctgc ccgaccttca ggccgttcgg tgcaatgatg tatctttttt
     2281 caccgtcctt gtataccacg agcgcgatgc gggccgagcg gttaggatcg tactccactg
     2341 tctttacaac accttcaatg ccgtgtttgt cgcgcttcat gtcaatgata cggaacatgc
     2401 gtttgtgtcc gccaccgatg tagcgcatcg tcattttacc ggagctgttt cttcccccgc
     2461 tcttcttgac gggaagcaga aggctctttt caggagttgc ggaagtgata tccgatgacg
     2521 taacaatcac gcggtggcgc attgcgggag tcagcggacg gaattttttt agtgccattt
     2581 ctctctattg tttaaaggat cagagattgc tgtaaaagtc aattacgtct ccctgcttca
     2641 gggtaacaac ggctttcttt tgccggttgg ccacaccaac ctgcattccg ctgcgcgtga
     2701 aacggctgcg aactttgccg atataatttt gcgtattgac ggtcttaacg gtaacgccat
     2761 acattttttc aacggcgttc ttgatctgca ttttatttgc gcgttgatca accacgaatc
     2821 cgtagcggga gaatttctcg ccctgcgcgg acattttttc agtgatgatc ggtttgatga
     2881 gcacgttcat ggcttagttc ttaagcaggt tttcaattgt ggataccgaa ctttccgaaa
     2941 gcaggatatt ggatgcgttc agaatatcgt aagtcgtaag ctcagcggct gtcaccacct
     3001 ttgcacgctc gaggttgcgc gacgacaggt aaacattctt gttatagttc ccggtaacaa
     3061 gaagcgtttt cttgtcagcg acattcagct tgccaagaat ttcgttgtac atgcccgtct
     3121 tcggtgcatc aaagctgagg tcctccagca cgagaatgtt attttctttc gccttatagg
     3181 agagcgcaga ttttctcgcc agcgccttta cttttttatt aaggcggatc ttgtaatcac
     3241 gcggttcggg tccgaaaaca cgtccgccac ctttgaacag gggaga
//
LOCUS       DASTSF010000106         8264 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1719083,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000106 DASTSF010000000
VERSION     DASTSF010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 8264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8264
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1719083"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..340
                     /locus_tag="VFU15_03195"
     CDS             <1..340
                     /locus_tag="VFU15_03195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716807.1"
                     /translation="LGFLIPLCDTTIVVINRLAKKKAPWIGGKDHTTHHLSYLGLSDS
                     QVAIAFCGMSVVSMMMIFVIQGTIVNWSPKHEMFFGGYILAVFGILFYVTKTRGNPAP
                     ADEAKSKQEE"
     gene            312..1508
                     /locus_tag="VFU15_03200"
     CDS             312..1508
                     /locus_tag="VFU15_03200"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEU4716808.1"
                     /translation="MKRKANRKSKKYTRWYKYLLALFVIVTFAFILNLIALSLSGNRT
                     DTDFRNAFLSHYKFNGVTLPKDMNFCGEAVPLDDFTVREELEKQLMLIAFNQPSTMLL
                     NKKAARWFPVIEPILKKNGVPEDFKYIAIIESGLVNSPDQPKAGGFWQLQIPVAQYYG
                     LEVNDEVDERFNIEKSTDAACKCFLDAYRHYHSWTLAAASYDMGMGALDKMIDKQKTS
                     DYYQLFLNEETSQYVYRLLAFKEIITRPKSYGYSLKPSQLYAPIPVHNVKVDSSVSDL
                     AAFAVKKGSTYKILKLLNPWLLKSSLTNPAHKTYYFQFPAKGAHLYGFETFEKNDSVP
                     DSVKPDIIVTDTLRAPRIYVVKEKDTWESIATAFGTDTNLLLKWNRRDGHDQPSPGEE
                     LVIPAK"
     gene            1616..4456
                     /gene="uvrA"
                     /locus_tag="VFU15_03205"
     CDS             1616..4456
                     /gene="uvrA"
                     /locus_tag="VFU15_03205"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012026474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEU4716809.1"
                     /translation="MAESENETLEVIGARVHNLKNLSVSLPRNKLVVITGLSGSGKSS
                     LAFDTIYAEGQRRYIETFSAYARQFLGDLERPDVDKITGLSPVISIEQKTVNKNPRST
                     VGTITEIYDFLRLLYARASDAYSYVTGEKMIRYSDDQIIDLILSSYEGKKIFLLAPVV
                     KGRKGHYRELFEQIRKQGFLRARVDGEIIELTTRVQLDRYKVHDIEIVIDRLAVSGDD
                     RQRIAESVRLALRHGKGTMMVSEPGKEEVRHFSRHLMCPVSGISYDDPAPNLFSFNSP
                     YGACPKCNGLGHILEIDINKIIPNRSRSIKSGAIVPIGEYRNSWIFKQLEALADKFRF
                     KLSDPVKDIPEDALNAILYGTDEMLKVKGDSFSSGTSYALSFEGVVNFLSRQEQEGSA
                     GVQKWAQEFMNEAQCPKCNGTRLKKEALYFRIDGKNIAELSAEGLSDLFTWLTGLEDR
                     ISEKQRTIAKEVLKEIRNRLHFLLDVGLDYLSIDRSSRSLSGGEAQRIRLATQIGSQL
                     VGVLYILDEPSIGLHQRDNMRLIHSLKELRDIGNSVIVVEHDKEMILAADHVVDIGPH
                     AGVHGGNVVAQGTPEELLKFDTLTADYITGKKEIAVPSQRRKGSGKSIVLDGCTGHNL
                     KNVTVEFPLGKFICVTGVSGSGKSSLINETLFPVLSRHFYRSERKPLPFRKIHGLEYI
                     DKVIDIDQSPIGRTPRSNPATYTNVFSDIRSLFAELPEAKIRGYKPGRFSFNVKGGRC
                     ETCQGAGLRTIEMNFLPDVYVLCESCNGKRYNRETLEVRYKGKSISDVLDMTIDAAVE
                     FFENHPSILQRVRALKEVGLGYITLGQQSTTLSGGEAQRVKLATELARKQTGNTLYIL
                     DEPTTGLHFEDVRILLEVLNKLADQGNTVLVIEHNMDIIKVADHIIDLGPEGGKGGGE
                     ILAEGTPEQVVKNKKGFTAEFLAKELHPPVHA"
     gene            4583..5290
                     /locus_tag="VFU15_03210"
     CDS             4583..5290
                     /locus_tag="VFU15_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017258577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEU4716810.1"
                     /translation="MTNEEKIRRAFAVKDWSEIKAGDSWQIFKIMSEFVEGFEKMQKI
                     GPCVSVFGSARTKPDNKYYKLAEEISEKLCREGYGVISGGGPGIMEAANKGAKQGKGK
                     SVGLNIRLPFEQFANIYVDPDKCINFDYFFVRKTIFLKYSQGFIAMPGGFGTLDELFE
                     SLTLVQTSKIAHFPVVLVGSDYWGGLIDWIRSTMLAKEHNVNESDLELFHLVDTADEA
                     VEIITAFYSRYMLKPNF"
     gene            5575..6855
                     /locus_tag="VFU15_03215"
     CDS             5575..6855
                     /locus_tag="VFU15_03215"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEU4716811.1"
                     /translation="MKCKSCAILIPASFFAAFSFAQTAPKELPLISMGAGMTYFSGDV
                     GKTTHTGGAYRSGFRFGIEQRFGNWFGAELFGFYGTLSKSERSLTLNRNFETPVICAG
                     LNGVIHLDNDVLLPKNSVLSPYLTAGFNWMSFDPHGDLKDAHGNPYYYWSNGSIHSVA
                     ENSPGAVLSPTVQRDYTYETKLTDSTNNYARTSFAVPVGAGLRFRFTPNFGANVQVSY
                     NITFTDYLDNVKDGGNDSWLWFGCSLYYKFGRNPDRIKSNDAGNRQFLNEDSDGDGVA
                     DLSDKCADTPKGVKVDGDGCPVDSDGDGVADYLDKEPGTKKGATVDANGVTIDFDKIA
                     AQAAKDSLNAAQKSNFLSNPGDSTLAQGDKDVKRSGTNGNCSIPDEFRPADANNDCII
                     TADEINKVIDHYFDGEGNWTVDSINRLIDYYFDQ"
     gene            6871..8259
                     /locus_tag="VFU15_03220"
     CDS             6871..8259
                     /locus_tag="VFU15_03220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716812.1"
                     /translation="MVRPLIIVVNLLIAFFLKFMTGTPSADITFPSTAKPGDKVNVKA
                     VIHTNGETGFIRFAMEVPEGWTVDTAKCSLDGSSFFFEKNTAKFIWSKPASSDLTVSF
                     KVVVPSSANGDYSFPCKISHTVNNLPDNIQLTPLKLNVSGSGSEEDLVKQAQDSTSKP
                     DVAVSAVRTVPPGDVTGEFSVDVLINKDDLGGWGKLEDSLPSGFLAQPDKNDGGEFHF
                     ENGVVRIYWYKMPSKTTLNVRYKVIASPDADGQKTIYGHFAYLENFGAKVIGITPSVV
                     NMKSSPALVVNDQNTNSGNKGNSSGTSQDSGNNGNTGTTGDQGQNSNTDNSGNNAKTN
                     NSGSENNAQAQNGNAQNNAQNSGQQNGNNSGSQGSKGVTYSVQIAAMKRMVPVSYYQS
                     TYGIAAVNAEQIDGLNKYTTGSFTSYNDARDHRETIRNKGVTGPFVAAYNSGKRITVQ
                     EALMITGQKWVP"
BASE COUNT         2081 a         2253 c         2157 g         1773 t
ORIGIN      
        1 actcggtttt ctcatcccgc tgtgcgatac caccatcgtg gtgatcaacc gcctcgcgaa
       61 gaaaaaagcg ccatggatcg ggggaaaaga ccacaccacg catcaccttt cgtacctggg
      121 actttccgac agccaggtgg caatcgcctt ctgcggaatg tccgttgttt ccatgatgat
      181 gatcttcgtt atccagggaa caatcgtcaa ctggtcgcct aagcacgaaa tgttcttcgg
      241 cggttatatt cttgccgttt tcggaatttt gttctacgtc acaaaaacca ggggcaaccc
      301 tgctccggca gatgaagcga aaagcaaaca ggaagagtaa aaagtacaca agatggtaca
      361 agtaccttct tgctcttttt gtaattgtca cgtttgcgtt tatcctgaat cttatagcgc
      421 tttcgctttc agggaaccgg acggacacgg acttccgcaa cgcgttcctt tcccattaca
      481 aattcaacgg cgtcacgctg ccgaaggaca tgaacttctg cggcgaagcg gttccgctcg
      541 atgattttac cgtgcgtgaa gagctggaaa aacaactgat gctgatcgcg ttcaaccagc
      601 cctcgacgat gttgctcaac aaaaaagcgg cgcgctggtt ccccgtcatc gaaccgatcc
      661 tgaaaaagaa cggggtgccc gaagatttca aatacatcgc gatcatcgaa agcgggcttg
      721 tcaattctcc cgaccagccc aaagccggcg gattctggca gctgcagatc ccggtggcgc
      781 agtattacgg actcgaggtg aatgatgagg tcgacgaacg cttcaacatt gaaaaatcga
      841 ccgatgccgc ctgcaaatgt tttctcgatg cttatcgtca ttaccacagc tggacgctgg
      901 ctgccgcctc ttacgatatg ggaatgggtg cgctcgacaa aatgatcgac aaacagaaaa
      961 cgtcggatta ttaccagctt tttctcaatg aagaaacatc acagtacgtt taccgtctgc
     1021 tggctttcaa ggagatcatc acccgcccga aatcatacgg ttattcgctg aaaccttcgc
     1081 agctgtatgc gccgattccc gtacataatg tgaaggtgga ttcttccgtt tccgatctcg
     1141 ctgcattcgc cgtgaaaaaa ggatccactt acaagatcct gaagctgctc aatccctggc
     1201 tgctgaaatc ttcgctcacc aatcccgcgc acaaaaccta ttatttccag ttcccggcca
     1261 aaggcgcgca tctttatggc tttgaaactt tcgaaaagaa cgattccgtt cccgactccg
     1321 tgaagccgga tattattgtg accgacacac tccgcgcacc gcggatttat gtcgtcaaag
     1381 aaaaagatac gtgggaaagc atagcaacgg cgttcgggac ggacacgaac ctgctgctga
     1441 aatggaacag gcgcgacggg catgatcagc ccagtccggg tgaagagctc gtgatcccgg
     1501 caaaataaag tttcattgca cttttttcgc ttccctcctg cccctttcag ccggaaaacg
     1561 cggtaccttt gcacgtattt ttctgcacgt gaaaacaacg aattcccttc cgcctattgc
     1621 cgaaagcgag aacgaaacac tcgaggtcat cggcgcgcgc gtgcacaacc tgaaaaacct
     1681 cagcgtgtca ctcccgcgga acaagctggt ggtcattaca gggctctccg gcagcggaaa
     1741 atcttctctt gcctttgaca cgatctacgc cgaagggcag cgtcgctaca tcgaaacgtt
     1801 ctccgcttac gcgcggcagt tcctcggcga ccttgaacgc ccggacgtgg acaagatcac
     1861 gggactttcc ccggtgattt ccatcgagca gaaaaccgtc aacaaaaatc cgcgctccac
     1921 tgtcggaacg atcaccgaga tctacgattt tctccgcctc ctgtacgccc gcgcttccga
     1981 cgcttactcg tacgtgacgg gcgagaaaat gatccgctat tccgacgacc agatcatcga
     2041 tcttattctt tcttcatacg aaggcaaaaa aatatttctt ctcgccccgg tcgtgaaagg
     2101 ccggaagggc cattaccgcg agctgttcga gcagatccgc aagcagggat tcctgcgcgc
     2161 acgcgtcgac ggggagatca tcgaacttac cacccgcgtg cagctcgacc ggtacaaagt
     2221 gcatgacatc gagattgtga tcgatcgtct cgccgtttcc ggggacgacc ggcagcgcat
     2281 tgcggaatcg gtgcggcttg cgctgcgtca cgggaaagga acgatgatgg tgagcgagcc
     2341 cggcaaggaa gaagtgcggc atttcagccg ccacctcatg tgtcctgtat cgggaatttc
     2401 ctacgacgac ccggcgccga accttttttc attcaactcg ccttacggtg cgtgcccgaa
     2461 atgcaacggt ctcggccaca ttctcgagat cgacatcaac aagatcattc ccaaccgcag
     2521 ccgctcgata aaatcgggcg cgatcgttcc gatcggtgaa tacaggaatt cctggatctt
     2581 caagcagctc gaagcgctgg cggataaatt ccgtttcaaa ttatccgacc cggtaaaaga
     2641 catcccggaa gatgcgctca acgccattct ttacggaacc gatgaaatgc tgaaagtaaa
     2701 aggcgattcc ttcagcagcg ggacgagcta cgcactttct ttcgaaggcg tggtgaactt
     2761 tttgtcgcgg caggaacagg aaggttcggc cggcgtgcag aaatgggcgc aggaattcat
     2821 gaacgaagcg caatgcccga aatgcaacgg cacgcgcctg aaaaaggaag cgctgtattt
     2881 ccgcatcgac ggaaaaaaca ttgcggaact ttccgcggaa ggactttccg atcttttcac
     2941 ctggctcacg ggactcgaag accgcatcag tgaaaagcag cgcacgatcg caaaagaagt
     3001 actgaaagag atccgcaacc gccttcactt cctgctcgac gtcggcctcg attatctttc
     3061 catcgaccgc agttcccgct cgctttccgg cggtgaggca cagcgcatcc gcctggcgac
     3121 gcagatcggg tcgcagctcg tgggcgtgct gtacattctc gacgaaccga gcatcgggct
     3181 gcaccagcgc gacaatatgc ggctcattca ttcgctgaaa gagctgcgcg acatcggcaa
     3241 ttcggtgatc gtggtggagc acgacaaaga aatgatcctt gccgccgacc atgtcgtgga
     3301 catcggtccg catgcaggcg ttcacggcgg gaatgtcgtg gcgcagggaa cgccggagga
     3361 actgctgaag ttcgatacgc taacggcgga ttacatcacc ggcaagaaag agatcgcggt
     3421 tccttcgcaa cgcaggaaag gcagcgggaa atcgatcgtg ctggacggat gcacgggaca
     3481 caacctgaag aacgtgacgg tggaatttcc attgggaaaa ttcatctgcg tcaccggtgt
     3541 gagcggcagc ggaaaatctt cgctgatcaa cgaaacgctt ttcccggtgc tgagtcgtca
     3601 cttttaccgc agcgaacgca agccgcttcc gttccggaaa attcacgggc tggaatacat
     3661 tgataaagtg atcgacatcg accagtcgcc gatcgggcgt acgccgcgtt cgaacccggc
     3721 gacctacacg aatgtttttt ccgacatccg ttccctgttc gccgaactgc cggaagccaa
     3781 aatccgcgga tacaagcccg ggcggttttc cttcaacgta aaaggcggaa gatgcgaaac
     3841 ctgccagggc gcaggattgc gcacgatcga gatgaatttc cttcccgacg tatacgtgct
     3901 gtgcgaaagc tgcaatggaa aacggtataa ccgggaaacg ctggaagtcc gttacaaggg
     3961 aaaaagcatc agcgacgtgc tcgacatgac gatcgatgcg gcggtggaat ttttcgaaaa
     4021 ccatccgtcc atcctgcagc gtgtgcgcgc gctgaaagaa gtcggcctcg gttacatcac
     4081 gctcgggcag cagagcacga cgctctccgg cggtgaagca cagcgcgtga agctggcgac
     4141 agaacttgcg cggaaacaga ccggcaacac gctttatatt ctcgatgagc cgacgacagg
     4201 cttgcatttc gaagacgtgc gcatcctgct cgaagtgctg aacaagcttg ccgaccaggg
     4261 caacacggta ctggtgatcg agcacaacat ggacattatc aaagtggccg atcacatcat
     4321 cgatctcggt cctgaaggcg ggaaaggcgg cggggaaatt ttagcggaag gaacgccgga
     4381 acaggtggtg aaaaataaaa aaggattcac cgcggaattc ctcgcgaaag agctgcatcc
     4441 gcccgttcac gcgtaacatt ccggcggcgt ttttcgtaac acaagcgtcc tctccccgcg
     4501 cccgcgccgt ttatggcaaa tgatttgtac tatcccccga aaaagcaccc actctccaaa
     4561 tctcaatact cagtactgat ttatgactaa cgaagaaaaa atccgccgcg cctttgcagt
     4621 aaaagactgg agcgaaatta aagccggcga ctcctggcag atattcaaga tcatgtcgga
     4681 atttgtggaa ggtttcgaaa aaatgcagaa gatcggtccg tgcgtttccg ttttcggatc
     4741 ggcgcgaacg aaacccgaca ataaatacta caagctcgcc gaagaaattt ctgaaaagct
     4801 ctgccgcgaa ggttacggtg tgatttccgg cggaggcccg ggaatcatgg aagccgccaa
     4861 caaaggtgcg aagcagggaa aagggaaatc cgtaggactc aacatccgtc tcccgttcga
     4921 acagttcgcg aatatttacg tcgatcccga caagtgcatc aacttcgatt actttttcgt
     4981 gcgcaagacg atcttcctga aatattcgca gggattcatc gccatgccgg gcgggttcgg
     5041 aacgctcgac gaattgtttg aatcgctcac actcgtgcag acgtccaaga tcgcgcattt
     5101 cccggtcgtg cttgtcggtt ccgattactg gggcggactc atcgactgga tccggtcgac
     5161 gatgctggcg aaagaacata acgtcaacga aagtgatctt gaattgtttc atctcgtcga
     5221 tacggcggac gaagcggtgg agatcatcac ggcgttctat tcgcgctaca tgctgaagcc
     5281 caacttctga tgctgcgtgt ttttcgcctg cggctttttg tgcacgagct tttgcgtcgt
     5341 tgtattctcc cgcaaaaacc gctcttaaca accggatgtt gataatcgaa attactttcc
     5401 ccggtgtggc cggcctcact gaatccctgc attcacaggg cttccggcga tttcgcacac
     5461 agcgtcatta acaaattcct gtttgtaagt acgatactgc cggcggaagc gcgttttcgc
     5521 ttcggatagc tttgccgtga ttcacccgtc tcattttcgc gtggatcaaa acccatgaaa
     5581 tgtaaaagct gcgctatcct gataccggct tcattttttg ctgcgttttc ctttgcgcaa
     5641 acggcgccga aggagcttcc gctgatcagc atgggagccg gcatgactta tttttccggc
     5701 gacgtcggga aaacgacgca caccggcggc gcgtaccgca gcggtttccg tttcggcatc
     5761 gagcaacgtt tcggcaattg gttcggcgcg gagcttttcg gtttttacgg cacgctttcc
     5821 aaaagcgaac gaagcctcac gctcaaccgg aatttcgaaa cgccggttat ctgcgcggga
     5881 ttgaacggcg tgatccatct cgataacgac gttctccttc cgaaaaattc cgtgctctct
     5941 ccttacctga ccgccggctt caactggatg tctttcgatc cgcatggcga tctgaaagat
     6001 gcgcacggta atccctatta ctactggtcg aacggctcca ttcactccgt tgctgaaaat
     6061 tcccccggcg cagttctttc accgactgtg cagcgtgact acacgtacga aacaaagctc
     6121 accgattcca cgaataatta tgcacgcacg tcattcgccg ttcctgtcgg tgccgggctg
     6181 cgtttccgtt tcaccccgaa cttcggcgcg aacgtgcagg tgagttacaa catcacgttc
     6241 accgattatc tcgataatgt aaaggacggt ggaaacgatt cctggctctg gttcggctgt
     6301 tcattgtatt acaaattcgg gaggaatccc gaccggatca aaagcaatga cgccggcaac
     6361 aggcaattcc tcaatgaaga ttccgacggt gacggcgtgg ccgacctcag cgacaaatgc
     6421 gcagatacgc cgaaaggtgt aaaagtggat ggcgacggat gtcccgtgga ttccgacggt
     6481 gacggcgttg cagattatct cgacaaagaa cccggtacaa agaaaggcgc aaccgtcgat
     6541 gcgaacgggg tgacgatcga cttcgataag atcgcggcgc aggcggcgaa agattcgctg
     6601 aacgcagcac agaaaagcaa tttcctcagc aacccgggcg attctacgct tgcacagggc
     6661 gacaaagacg tgaagcgcag cggcacgaac ggcaactgca gcattccgga tgagttcagg
     6721 cctgcggatg caaacaacga ttgcatcatc accgccgatg agatcaacaa ggttatcgat
     6781 cattatttcg atggtgaggg aaactggacc gtcgacagca tcaaccgcct tatcgattat
     6841 tatttcgatc aataaaaaag aaaaaacgtt atggtacgac cactgatcat cgtagtaaat
     6901 ctcctgatcg cgtttttcct gaaattcatg acgggcactc cgtcagccga catcactttt
     6961 ccttcaacgg caaaaccggg cgataaggtg aacgtcaaag ccgttattca taccaacggg
     7021 gaaaccggct tcatccgttt tgcgatggaa gtccccgaag gatggacggt ggatacggca
     7081 aaatgctcgc tcgacggatc gtctttcttt ttcgaaaaga acacggcgaa gttcatctgg
     7141 tcgaagcccg catccagcga tctcactgtt tctttcaagg tagtggttcc ctcttccgcg
     7201 aacggagatt attctttccc gtgcaaaata tcgcacacgg tcaacaatct ccccgacaat
     7261 atccagctca cgccgctcaa gctgaacgtt tcgggcagcg gctctgaaga agatcttgtc
     7321 aagcaggcac aggattccac gtccaaaccc gacgtcgctg tttccgcggt gcgcacggtt
     7381 ccgcccggcg atgtaaccgg cgaattttct gttgacgtcc tcatcaacaa agacgacctg
     7441 ggcggatggg gaaagctgga agattctctt ccttcgggtt ttcttgcgca gccggataaa
     7501 aatgacgggg gagaatttca tttcgagaac ggcgtcgtgc gcatttactg gtacaagatg
     7561 ccttccaaga ccacgctcaa cgtacgttac aaagtcatcg catcaccgga tgcggacggg
     7621 cagaaaacca tttacggcca tttcgcctac ctcgagaatt tcggtgcgaa ggtgatcggc
     7681 atcacgcctt ccgtggtaaa tatgaaatcg agcccggcgc tcgtggtgaa cgaccagaat
     7741 actaattccg ggaacaaagg gaattcgtcc ggcaccagcc aggattcggg aaacaacgga
     7801 aatacgggga caaccggtga tcaggggcag aacagcaata cggacaattc cggcaacaat
     7861 gcgaaaacga acaattccgg ttcggaaaac aacgcccagg cacagaacgg gaatgcgcag
     7921 aacaatgcac agaacagcgg acagcagaac gggaataatt ccggcagcca gggatcgaaa
     7981 ggcgttacgt acagtgtgca gatcgcggcg atgaagcgga tggtcccggt ttcctactac
     8041 cagagcacat acggaattgc agcggtaaat gccgaacaga tcgacgggct gaacaaatac
     8101 acgaccggga gctttaccag ctacaacgat gcgcgtgatc accgcgaaac gatccggaac
     8161 aaaggagtga cggggccgtt cgttgccgcg tacaattccg gcaaacggat cacagtgcag
     8221 gaagcgctga tgatcaccgg ccagaaatgg gttccgtagt gccc
//
LOCUS       DASTSF010000107         7820 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1943442,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000107 DASTSF010000000
VERSION     DASTSF010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 7820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7820
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1943442"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..564
                     /locus_tag="VFU15_03225"
     CDS             <1..564
                     /locus_tag="VFU15_03225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716813.1"
                     /translation="GSTGGRRGRRGSGGRGSTRGRQSSSASSSQQEAEALAAPVIAAP
                     AHPVDAVGMVLHYHGAIAWANAQGTYSDEFHFVRNNPAAARLLLQNGGMTAAAALAFT
                     QQFAVAVQHFGQQLVGYVPAEMQEQILNTLGAQEQLLAVIRYLQHGGGEEQQQPEQRQ
                     EQQPEQRQEQQPEQRQEQQQQEDLEDL"
     gene            599..2512
                     /locus_tag="VFU15_03230"
     CDS             599..2512
                     /locus_tag="VFU15_03230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716814.1"
                     /translation="MKFFTGRKQESTGRHPAQQTGGGNSARALVDNRPSSVLQQKHFP
                     ATSQLKGKVNINDDASLEKEADKMGAMAMNTGPGNSVQRVSSFLPSSAGTKTVQGKWF
                     RMSSILTEEDTQIVEKIQQLANEGQYLEAFQVVIEPYESLQQDLRVIVYLAEKYGRMD
                     EVTGLLHRTFTSGGKAYTAGEIIEELDALFEEQKLHDEEFHEEVGAEPLSPQERGHLA
                     IEEIMQLTQQVNDTEELMQQMSPIAEKYGITEYHLDTEKHPAVLMFRINPQFQIVLGG
                     GHAIEMRSEGSGGAVQTKVRWQPQKLTLSTIAHPGGGTYTVGKNMLANPLSQDHPAGT
                     IANADSDHDGLMGKLPSKGNRKKTGSGSGPYFFIKGHLLNDNLGGIANEANLFPITHE
                     ANGQHKNYVEQYIKKGIGEGYVYRYEVDIQNIAVDYDNGLNLYSVDSDIAFSFARLDT
                     SLNDVPGTVHNGRIQSRYENVGAQPFDKSTEYSKDYGGSYNHPVKVGTEAVSKSTEKT
                     PLGSNSGLFSHVSAFTMGLPSGSSAFSGGSGGGMSISTSAALVPSGKLLSLRKSNITS
                     VENYFAQLVNGWSKTDITGWITDVRGNNLSSWARVWDSATTSFNLDPAVAANITSGGY
                     LVRVSINGKATGT"
     gene            2587..3951
                     /locus_tag="VFU15_03235"
     CDS             2587..3951
                     /locus_tag="VFU15_03235"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEU4716815.1"
                     /translation="MKFRALLFLLLSCFLFTGIYAQAPCDVISVLPNLNPAPLYSPDS
                     SMYLINKPDTAGVFQVYVGHTGDTAATCISLVNPWSLWRPWSQRNKMQVQWSPTGNFI
                     ICGVEKEFYNELLYVPYSIRLGLLESGIWMDIMACTPDGNTWYNLATTIHGFTGPAFT
                     PAGTQCAWAEALDSSNLSVDVFGDWKLRLSQYSETSGTPSFSSTNDITPAGARWIEPG
                     NFSPDGVSLLVSSDIGLINAEGQDQYIINISNGQVTNLTNSPIIWDEHGVFSPDGTKI
                     LFMSSYPYQADTNSYHTLTIKTEFMLMDPDGMNLQQLTHYCDTGYVESGAGIAATGFW
                     GHGDSTIYAQSLLFPAYNDWKIKFHGDCGYSVTSVNEISSPGGVVLYPDPAGNMLTVQ
                     TDFLLHDATVILWNATGQMVKIIPGAGGNTVHILTNDLPPGIYFLGIQNGSQTVTKKF
                     VKAG"
     gene            4047..5246
                     /locus_tag="VFU15_03240"
     CDS             4047..5246
                     /locus_tag="VFU15_03240"
                     /inference="COORDINATES: protein motif:HMM:NF026220.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HEU4716816.1"
                     /translation="MKKLFSFLLPALSAGTLSAQWTPVSTAMSGKLETVVFTSPSDGF
                     AAGSFQYMYKTVDGGSTWLSAGSYDAKDIYFFDANHGYAAGVAGTGTMKSTTNGGSSW
                     TTLTPPNSSSIWGTFATSASTCYFIATDKKVHKSTNSGGSFTSVTLSITFSPTDIWFT
                     DANTGFITTQDGIIFKTTNAGGSWSNSFSVSGVQLNSIYFVNTNLGFACGTNGAVLKT
                     TNGGTSWTALVTGTTSPLNCVRFYDALNGLAVGYGGTIIRTADGGTTWTSDNSNTTRW
                     LYSISYTSSAAAAVIVGDSGTVLKNTNIPIGISEPAASLSSINCFPNPANGYISVSFR
                     ATKTADISPVIYDLDGRAVKSFSAFTASPGDNDIRLDVADLSPGAYLVRLDADDQPAF
                     RKILITR"
     gene            complement(5289..6338)
                     /locus_tag="VFU15_03245"
     CDS             complement(5289..6338)
                     /locus_tag="VFU15_03245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716817.1"
                     /translation="MLVFLLAGTSGRAQGCSDGGLCSFGTMGILQPRYVNIPLDQVKL
                     TPMNVVDSAALSYTHVRDTSMPAGAGGNAYVVQAKSVDTGYVSHITSCELSAYYGIGE
                     HNTSVIITQLEGNFNLVNRKLYGQVKIPYSVVSGNLGTTNGLSDITMSLSYVAFDKGR
                     SSMSFAAGVKIPSNSSDLSKDNRPLPMVYQTSLGSTDILLGTRFAYRKWDFSAGYQHS
                     FNGNSNHYVHIPGAGDSLTYNGYFESNNLRRADDAMFRINRKFQAKKIELNTGLLFIF
                     HLANDDYTDATGNRVKATGSQGLTLNFDLAGSIPVGKKTDLTFILAKPLKQRSYVTDG
                     LARSFVGIVGIKQNF"
     gene            complement(6485..6652)
                     /locus_tag="VFU15_03250"
     CDS             complement(6485..6652)
                     /locus_tag="VFU15_03250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716818.1"
                     /translation="MKKIRAGVPGLTGIPVTSGMKSVFLSLCLPLISFAAKAGDAESA
                     DPFRKRTVVKM"
     gene            6698..7780
                     /locus_tag="VFU15_03255"
     CDS             6698..7780
                     /locus_tag="VFU15_03255"
                     /inference="COORDINATES: protein motif:HMM:NF026121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4476 domain-containing protein"
                     /protein_id="HEU4716819.1"
                     /translation="MKHFSIALLSALLITANVFAQDCQDPVSPAVFQSNFNVIAVQHT
                     NQKKLEQADLFVNSNCLTAAQVKNIAQLFTDDSYRLDFCKNAYLHTFDRVNFYDVYDT
                     FTKFSYAFRLNDYIHELDSNTASNTTTVTVNPPPHAAPVFPNYSYPAIAAYNGKKGCA
                     GPVISDDAFNNIAQNVFSQPTDESKVVAIQSASNANCLSFAMIMKLSSLVGNESLRLS
                     VMENTFSKVFDLENYPSGTQLFTDNNNQAAWLNYAKNTLTPPPAPCVTSDADFNTVLA
                     SVHSKPFDNDKMSTLQLAAKDRCFSVAQIRKLAAEFAFSDAKLKVFKMLYAKCNDQQD
                     YYQCLDDLTFSTEKDDLSNFIKNGGK"
BASE COUNT         1997 a         2153 c         1963 g         1707 t
ORIGIN      
        1 ggcagcacag gcggaagacg tggcaggcgc ggaagcggcg gaagaggatc cacccgcgga
       61 agacagtctt cctccgcatc atcttctcaa caggaagcgg aagcactggc tgccccggtg
      121 atagcagctc ccgctcaccc cgtggatgcg gtagggatgg tgcttcatta ccacggagcg
      181 atcgcctggg ccaatgcgca gggaacctac agcgacgaat ttcatttcgt acggaataat
      241 cccgcggcgg caagacttct tctgcaaaac ggcggcatga cagcggcagc ggccctcgca
      301 tttacgcagc aattcgccgt tgccgtacag catttcgggc agcagctcgt tggatatgta
      361 cctgcggaaa tgcaggaaca gatcctgaat acgcttggtg cgcaggaaca acttctcgcc
      421 gtaatacgtt acctgcagca tggcggaggt gaagagcagc agcaaccgga acaacgacag
      481 gaacagcaac cggaacaacg gcaggaacag caaccggaac aacggcagga acaacaacag
      541 caggaagacc tggaggatct ttaaacagaa aaaacacctc cgtttcgcag gttcaatcat
      601 gaagtttttt acaggcagaa aacaggaaag tacaggccgt catcccgcgc aacaaacagg
      661 cgggggaaac tctgcgcgcg cactcgtcga caaccggcct tcttctgtcc tgcagcaaaa
      721 acatttcccg gcgacatcgc agctgaaagg aaaagtgaac atcaatgatg atgcttcgct
      781 ggaaaaagag gcggataaga tgggggccat ggcgatgaat accggcccgg gaaattccgt
      841 acaacgcgtc tcttcttttt tgccatcatc cgcaggaacg aagacagtgc agggaaaatg
      901 gttccggatg agttctatcc tgacagaaga agatacccaa atcgttgaga aaattcagca
      961 gctggcgaac gaaggacagt accttgaagc gttccaggtt gtgatcgaac catacgagtc
     1021 gctgcagcag gacttacgcg taatcgtata cctggcggaa aaatatggcc ggatggacga
     1081 agtcaccgga ctgctgcata gaacatttac gtccggtgga aaagcgtaca ccgcaggtga
     1141 aataatcgag gaactcgatg ctcttttcga ggagcaaaaa ctgcacgatg aggagttcca
     1201 tgaagaagtt ggggccgaac ctctttcgcc acaggaaagg ggacacctcg caatcgagga
     1261 aattatgcaa ttgactcagc aggtcaatga cacggaagaa ctgatgcagc aaatgagtcc
     1321 cattgcagaa aaatacggta ttacagaata tcacctcgac acggaaaaac atcctgcggt
     1381 tctgatgttc cgcataaatc ctcaattcca gattgtgctg ggcggcggtc acgccatcga
     1441 aatgcgatcg gaaggaagcg ggggggcggt gcagacaaaa gttcgctggc aaccgcaaaa
     1501 gctcacactg agcacgatcg ctcatcccgg gggaggaacg tataccgttg ggaaaaatat
     1561 gctcgcgaac ccactcagcc aggatcatcc ggcgggaaca attgccaatg cggacagtga
     1621 tcacgacggg ttgatgggca agcttccttc caaaggcaac cgcaagaaaa caggctccgg
     1681 atctggtccc tatttcttta tcaaagggca tttgctcaat gataatctcg gtggaattgc
     1741 aaatgaagca aacctgttcc ctatcacgca cgaagcgaac gggcagcaca aaaattacgt
     1801 cgagcaatac atcaagaaag ggatcggcga aggatatgtt taccggtatg aagtcgatat
     1861 tcagaatatc gcggtggatt acgacaacgg acttaacctg tattcggtcg attcagatat
     1921 tgcattcagt tttgcgcgcc tcgatacgtc actgaacgat gttccgggaa cggttcacaa
     1981 tggcaggata caatcgagat acgaaaatgt aggagcccag cctttcgaca aaagcacgga
     2041 gtattccaaa gattacggcg gcagctataa ccatcctgta aaagtcggca ccgaagcggt
     2101 tagcaaatca acggagaaaa caccgctggg cagcaacagc ggactcttct cccatgtttc
     2161 ggccttcacg atggggcttc cttccggttc cagcgctttc agcggcggtt cgggtggtgg
     2221 aatgagtatc agcacatctg ctgctcttgt tcccagtggt aagctacttt cgctgcgcaa
     2281 atcgaacata accagcgtgg aaaattattt cgcccagctc gtgaacggat ggagcaagac
     2341 ggatatcacc gggtggataa ccgacgtccg cggtaacaac ttatcctcct gggccagggt
     2401 atgggacagc gccaccactt cttttaacct tgatcccgcc gtcgcggcca atatcacgtc
     2461 cggcggctac cttgtccgtg tcagcatcaa tgggaaagca accggaacct gatcattcct
     2521 gtgcgctgca tgtctcgccg gaacaaaaaa cgctttcctt ttcggcaaac gcactatctt
     2581 ttcacaatga aattccgcgc gcttttgttc ctcctgcttt cctgttttct tttcacagga
     2641 atatacgccc aggccccctg cgacgtcatc agcgttcttc ccaacctgaa tcctgctcca
     2701 ttgtactcgc cggacagctc catgtatctc atcaacaaac cggatacggc cggcgtgttc
     2761 caggtttacg tcggtcacac cggtgatacg gcggcgacct gcatctcgct cgtgaacccg
     2821 tggagcctgt ggcggccctg gagccagcga aacaagatgc aggtgcaatg gtcgccgaca
     2881 ggaaatttca tcatctgcgg cgtggaaaaa gaattttaca acgagctgct gtatgtcccg
     2941 tattccatcc gcctcggcct gctcgaaagc gggatatgga tggacatcat ggcctgcaca
     3001 cccgacggaa atacgtggta caatctcgcc acgacgatcc acggatttac cggcccggcg
     3061 ttcacgcccg cgggaacgca atgcgcctgg gcggaagctc tcgacagcag caacctttcc
     3121 gtcgatgtgt tcggcgactg gaagctgcgt ctttcccagt attcagaaac aagcggaacg
     3181 ccttcgttct cgtcgacaaa tgacatcaca cctgcaggag cgcggtggat cgagcccgga
     3241 aatttttctc ccgacggcgt ttccctgctc gtttcttccg acatcggtct catcaacgca
     3301 gaaggacagg accagtacat catcaacatt tcgaacgggc aggtaacgaa cctcacgaac
     3361 agcccgatta tctgggatga gcacggtgtg ttttcacctg acggaacgaa aattcttttc
     3421 atgagctctt atccttacca ggccgacacg aattcgtatc atacgctcac gatcaaaacc
     3481 gaattcatgc tcatggatcc ggatggaatg aacctgcagc agctcaccca ttactgcgac
     3541 acgggttatg tcgaatcggg tgcgggaatt gcggctaccg gtttctgggg gcacggcgat
     3601 tcgacgatct acgcacagtc gcttttattt cctgcttaca acgactggaa aataaaattc
     3661 cacggcgatt gcgggtattc cgtcacttca gtgaatgaaa tttcttcccc gggcggagtc
     3721 gtactgtacc cggatcctgc gggcaacatg ctaaccgtgc aaacggattt tctcctgcac
     3781 gacgcgacgg tcatcttatg gaatgcgacc ggacaaatgg tgaaaataat tcccggcgcc
     3841 ggcggaaaca ccgtgcatat tcttacgaac gatctccccc cgggcattta ttttctcggg
     3901 atccaaaacg gcagccagac cgtcacgaaa aaattcgtga aagcaggttg atttttttcg
     3961 tcccgcacgc ttgatccgtg ctgcacgctt ccggatcttc agccatgttt cttctttttt
     4021 ataccttgag aaaaaataac cctatcatga aaaaactttt ctcatttctt cttcctgcac
     4081 tttccgcagg aacgctttcc gcacaatgga caccggtcag cacagccatg agcggcaaac
     4141 tcgaaacagt ggttttcaca agcccttccg atggatttgc agcaggctcg ttccagtaca
     4201 tgtacaaaac cgttgacggg ggaagcacat ggctcagcgc cggaagctac gacgcaaagg
     4261 acatttattt cttcgatgca aaccacgggt acgccgccgg tgtggcggga acaggtacaa
     4321 tgaaaagcac aacgaacggc ggaagctcct ggacaactct cacgccgccg aattccagct
     4381 ccatctgggg aacgttcgca accagcgcct ccacgtgcta tttcatcgca acggataaaa
     4441 aagtgcacaa gtccaccaac agcggtggtt ccttcacatc tgtaacgctc tccattactt
     4501 tttcacctac cgacatctgg tttaccgatg caaataccgg gttcatcacc acgcaggacg
     4561 gcatcatttt caaaaccacg aatgccggcg gctcctggtc gaacagcttt tccgtttccg
     4621 gcgtgcagct caattccatt tatttcgtca atacaaatct tggctttgcc tgcggaacca
     4681 acggcgccgt cctgaaaaca accaacggcg gcacatcatg gacagcgctc gtaaccggca
     4741 cgacctcccc gctgaattgc gtcaggttct acgatgcgct caatggcctg gcggtcggtt
     4801 acggaggaac gatcatccgc actgcggacg gcggcaccac gtggacatcc gacaattcga
     4861 acacgacgcg ctggctgtac agcatctctt atacttcttc cgccgctgca gccgtcattg
     4921 tcggcgacag cggaacggtg ctgaaaaaca cgaatatccc gatcggcatt tccgaacccg
     4981 ctgcatcgct cagctccatc aattgttttc ccaatcccgc aaacggatac atcagcgttt
     5041 cgttccgcgc aacgaaaacc gcggatatat cgccggtgat ctatgatctc gacgggcgcg
     5101 ctgtaaaatc tttttccgca ttcaccgctt cccccggcga caatgatatc cggcttgatg
     5161 tcgcagatct ttcaccgggc gcctacctcg tcaggcttga tgcagatgac cagcctgcat
     5221 tcaggaaaat cctgatcaca cggtaaatca tacagagaaa gcaaaggaaa aacgggcaag
     5281 agaaaggatc agaaattctg cttgatcccg acgatgccca cgaagctgcg cgcgagtccg
     5341 tccgtgacgt acgaacgttg cttcagcggt tttgcaagaa tgaacgtgag gtctgtcttc
     5401 ttgccgaccg ggatgcttcc cgcgagatcg aaattgagcg tgagtccctg cgagcctgtt
     5461 gctttgacgc gattgccggt agcgtccgtg taatcgtcgt tcgccaggtg aaagatgaaa
     5521 agcaatcccg tgttcagctc gatctttttc gcctggaatt tccggttgat cctgaacatc
     5581 gcatcatccg cgcggcgaag gttgttcgat tcgaaatagc cgttgtaggt taagctgtcg
     5641 cctgcgccgg gaatatggac atagtgattc gaattgccgt tgaacgaatg ctggtaaccg
     5701 gcggaaaaat cccatttgcg gtatgcgaaa cgcgtgccga gcaggatgtc ggtgctgccg
     5761 agactcgtct ggtaaaccat cggcagcggc cggttgtctt tcgaaagatc ggatgaattg
     5821 cttgggattt tcacgccggc ggcaaagctc atgctggatc ttcctttatc gaaagcgacg
     5881 tagctgagac tcatcgtgat gtcgctcaac ccgttcgttg tcccaagatt cccgctgaca
     5941 acggaataag gaattttcac ctggccgtac aatttccggt tgacgagatt gaaattccct
     6001 tcgagctggg taatgataac ggaggtatta tgctcgccga tcccgtaata agcggagagc
     6061 tcgcagctgg taatgtggga aacgtaaccc gtgtcgacgc ttttcgcctg caccacgtaa
     6121 gcgtttccgc ccgctcctgc aggcatgctc gtatcgcgca catgcgtgta gctcagcgct
     6181 gccgaatcca cgacattcat cggtgtcagc ttcacctggt cgagcgggat gttcacgtaa
     6241 cgcggctgaa gaattcccat cgtcccgaaa gagcagagtc cgccgtcgct gcagccctgc
     6301 gccctgccgg acgtccctgc aagcaaaaaa acgagcataa aaaaaagaaa gccggaaacc
     6361 ttcttccttt tgttcgtgca gtcggtaaat aatccgatgg tattcaaaac gtggtcagaa
     6421 atgcaacggg tgaaattcgt tctttccgaa agtacaaaaa atagcttcag gacattgtgt
     6481 gagttcacat tttgacaacc gttcgctttc gaaacggatc cgcagattct gcatccccgg
     6541 ctttcgcggc aaaagaaatc agcggcaggc acaaggacaa aaaaacactt ttcattccgg
     6601 aagtaacagg tatcccggtt aagccaggca cgccggcacg gatctttttc atttctttgg
     6661 catcaaaatt gaaaagcatc ccacaaaaaa gaatctgatg aaacattttt ccattgccct
     6721 cctctctgca ttgctcatca ccgcgaacgt attcgcacag gactgccagg atcccgtttc
     6781 ccctgctgtt ttccagagca atttcaacgt cattgccgtg cagcacacga accagaaaaa
     6841 gctcgagcag gccgatcttt tcgtgaatag caactgcctc acggccgcgc aggtgaagaa
     6901 catcgcgcag ctgttcaccg acgattcata ccgcttggat ttctgcaaga atgcttacct
     6961 gcatacgttc gaccgcgtga atttctacga cgtgtacgat accttcacga aattctccta
     7021 cgcgttccgc ctgaatgatt acatccatga gctggacagc aacacggcca gtaatacgac
     7081 taccgtcacg gtcaatccgc ctccgcatgc ggcgccggtt tttccgaact acagctatcc
     7141 tgccatcgcc gcttacaatg gtaaaaaagg atgcgcgggt cctgtgatca gcgacgacgc
     7201 attcaacaac attgcacaga atgttttcag ccagccgacg gatgaatcga aggtggtcgc
     7261 cattcaatcg gcctccaatg caaactgcct ttctttcgcg atgatcatga agctgagctc
     7321 gctcgtcggc aatgagtcgc tgcggctgag cgtgatggaa aatacgttca gcaaagtgtt
     7381 cgacctggaa aattatcctt cgggcacgca gctgttcacc gacaacaaca accaggctgc
     7441 atggctgaac tatgcgaaaa acacgctcac gcccccgccc gctccgtgcg ttaccagcga
     7501 tgccgatttc aataccgttc tcgcttccgt ccattccaaa ccattcgaca acgataaaat
     7561 gagcacgctg cagctcgccg caaaagaccg ctgcttcagc gtggcgcaga tccgcaagct
     7621 cgccgctgaa ttcgccttca gcgatgcgaa gctgaaagtt ttcaaaatgc tgtacgcaaa
     7681 atgcaacgac cagcaggatt actaccagtg cctcgatgat cttacttttt ccacggaaaa
     7741 agacgacctg agcaatttca tcaagaatgg cgggaagtga tcgcttcttt tcacaggaac
     7801 aaacaggctc ccggtttttg
//
LOCUS       DASTSF010000108         5973 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1716979,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000108 DASTSF010000000
VERSION     DASTSF010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5973)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5973)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5973
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1716979"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..236
                     /locus_tag="VFU15_03260"
     CDS             <1..236
                     /locus_tag="VFU15_03260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716820.1"
                     /translation="RELQRFLRHCQRGGVLYVPYAERVPVQGVGLFPCIGDFPDDLFY
                     FRCCFQEFRLTLSKIKFRGEEQNITGVSLLRAY"
     gene            complement(205..876)
                     /locus_tag="VFU15_03265"
     CDS             complement(205..876)
                     /locus_tag="VFU15_03265"
                     /inference="COORDINATES: protein motif:HMM:NF020296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HEU4716821.1"
                     /translation="MELGRYITAMEKEFRRHASAKQAAGAKAYMRNQFEYTGLSTPVR
                     RRLLKEFIRQNGIPTPGQMEEFVMLMWKKEEREFQYSAMEIFHLQKKNFRKSDLAVFG
                     YMITHRPWWDTVDFIAPKLAGEYFRLFPREIKKTIRQWLSSGNTWLIRSAILFQLNYK
                     SETDEKLLFSICSSQSMHEDFFIRKGIGWALRQYARTNPSAVRRFVSTHKLSPLSKRE
                     AMKHL"
     gene            complement(877..2145)
                     /gene="nhaD"
                     /locus_tag="VFU15_03270"
     CDS             complement(877..2145)
                     /gene="nhaD"
                     /locus_tag="VFU15_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019989730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter NhaD"
                     /protein_id="HEU4716822.1"
                     /translation="MLLASVFILGYLAIAFEQTLKVNKSASALLTGVVCWMIVIAGTN
                     AGLIDSKLGVQLSGISEILFFLLGAMAIVELIDAHDGFTLITEKLRTKSKRKLLFLVS
                     LITFLLSSVLNNLTTALVMMSLVRKLVKDPTDRMYYGGMIIIAANAGGVWSPVGDVTT
                     TMLWIGGQISSLNIILKLFVPALVSLFLPYLWLRRKMSGNIEPPENEKTYSRPTTRFE
                     QNLVLFTGAAVLLLVPVFKSVTLLPPYMGILFGLGILWSLTEIIHKQKNDEVKSSLSV
                     AGALQRIDTQSILFFFGILLAIGALDAKGTLVSLAEHLHSSIGNLNLITLSIGLSSAI
                     VDNVPLVAAAQGMYPLAEFPRDFYFWEFLAYCAGTGGSILIIGSAAGVAVMGTEHISF
                     GWYLRKISVPAIIGYFGGALAYIAIVEILH"
     gene            2277..3752
                     /locus_tag="VFU15_03275"
     CDS             2277..3752
                     /locus_tag="VFU15_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007810861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEU4716823.1"
                     /translation="MSIFRKKSLETLLAQAAESEKGLKRTLGSTQLVALGIGAIIGAG
                     LFVRTAAAAGEAAGPGVTLSFIVAAIGCAFAGLCYAEFSAMIPIAGSAYTYAYATMGE
                     LIAWIIGWALILEYALGAATVSIAWSEYLNKLLGGHIPFQWCHGPFESATVDGTLVHG
                     IVNAPALFILLALSLLLIRGTQESAFVNAIIVFLKVAIVLVFIAIGWQFIKPENHTPY
                     LIPADYPGHAGIFQHGWGGILKGAGIVFFAFIGFDAVSTAAQEAKNPKRDMPIGILGS
                     LVVCTILYILFSHVLTGVAPWADFNDPAKGKEASVAYAISTYMQGYGWLATAVTIAIL
                     AGFSSVILVMLMGQSRVFYSMSHDGLLPKIFSHLHPKFRTPYKSNILLFCFVGLFGGF
                     IPGSVAGDLTSIGTLLAFVLVCAGIWIMRVNSPGAVRPFRTPGASPSFPIVPILGIVV
                     CSFMIISLDRLTQISAFGWMIFGLIIYFLHGKKNSKLNVLQ"
     gene            3808..5271
                     /locus_tag="VFU15_03280"
     CDS             3808..5271
                     /locus_tag="VFU15_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009280292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEU4716824.1"
                     /translation="MEEKREFRRSLGLIDATALVAGSMIGSGIFIVSSTMAQDLGCAG
                     WLIVAWIMTGVITLTAALSYGELAGMMPEAGGQYVYIRRAWGELPAFLYGWTVFSVIQ
                     TGVIAAVATAFANYTSELVPALSMKNVLLDAGFIKISSGQVFAVAMIFFLSWINSRGV
                     QGGALVQKVFTSAKLIALFGLIILGISIGLKSGNLAHNFTDPWMATRIRVSDSGSYSL
                     TPLTGFALLAVFGMTMINSLFSADAWNNVTFIAGEIREPHKNIPRSLLLGTAIVTGLY
                     VLANIAYLALLPLKGDPGGTDVMSRGMQFAEMGRVGTAAATMIFGKIATIMMAVLIMV
                     STFGCNNGIILAGGRLYYAMAKDGLFFDRIAKLNRKNVPASAIWIQAVWGSLLCFSGT
                     YNDLLTYATFASMLFYIVTISGLFRLRKIEPGTPRPYKALGYPLLPAFYILAAAGICI
                     DLLIMDPRNSWAGLGIVALGIPVFFIRKAFPSQKTAV"
     gene            5337..>5973
                     /locus_tag="VFU15_03285"
     CDS             5337..>5973
                     /locus_tag="VFU15_03285"
                     /inference="COORDINATES: protein motif:HMM:NF024245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEU4716825.1"
                     /translation="MRNWFRNTFSFTRRERNGAAVLIVILIALSAFTAVSSRFVSRPQ
                     EDFSGFDKFIAQVKQDSLFDSLAGKNTENKPPSIAEEEADHVFHQRELFQFDPNGLPV
                     SDWVRLGLTPAQARSVKHYEESGGKFESKEDVKRLYVVSPKLYADLEPYILLPDKKEN
                     DARPDKFPAKKNPPAILELNTADSALLVTVNGIGPVFAKRIIEYREKLGGFY"
BASE COUNT         1276 a         1619 c         1611 g         1467 t
ORIGIN      
        1 tccgagagct tcagcgcttc ctgcgccatt gccagcgcgg tggcgtgctt tatgttccgt
       61 atgctgaacg cgtgccggtt cagggcgtcg gtcttttccc ttgcatcggt gattttcccg
      121 atgacctctt ctatttcaga tgttgtttcc aggaatttcg tcttacttta tcaaagataa
      181 aattccgggg ggaggaacaa aatattacag gtgtttcatt gcttcgcgct tactgagcgg
      241 ggacaactta tgcgtgctga cgaagcggcg gacggctgaa ggatttgtcc gggcgtattg
      301 gcgcagcgcc cagcctatgc ctttgcggat aaaaaaatcc tcgtgcatgc tttgcgatga
      361 gcaaatggaa aaaagcagct tttcgtcggt ttcgcttttg taattcagct ggaaaagtat
      421 cgccgaacgg atcagccagg tgttcccgga tgaaagccat tggcggatcg tcttttttat
      481 ttcccgcggg aaaagacgga aatattctcc ggccagtttg ggcgcaatga aatccacagt
      541 gtcccaccac ggtctgtgcg tgatcatgta tccgaatacg gcgagatcgc ttttccggaa
      601 attctttttt tgcagatgaa aaatttccat tgcggaatac tggaattcgc gttcttcctt
      661 cttccacatc agcatcacga actcttccat ttgtcccgga gtgggaattc cattctgccg
      721 tatgaattct ttcagcaatc ttctgcgcac aggcgtggaa agcccggtgt attcgaactg
      781 gttgcgcatg tacgctttcg caccggcagc ctgttttgca ctggcgtgcc tgcgaaactc
      841 tttttccatt gcggtgatgt atcgccccag ctccattcag tgaagaattt ccacgatggc
      901 aatgtaagcc agcgctccgc cgaaatagcc gatgatcgcc gggaccgata ttttgcgcag
      961 gtaccagccg aaggaaatgt gctcggttcc cattaccgcg acgcctgccg ccgacccgat
     1021 gatgagaatg cttccgccgg ttcccgcaca gtaggccagg aattcccaga aataaaaatc
     1081 acggggaaat tccgccagcg ggtacatgcc ctgcgccgcc gcgacaagcg ggacgttgtc
     1141 gacgatcgcg gaggaaagcc ctatgctcag cgttatgagg ttgaggttcc cgatcgagga
     1201 atgcagatgt tcggcgaggg aaacaagcgt accttttgca tcgagcgccc cgatcgccag
     1261 caggatgccg aagaaaaaaa gaatgctctg cgtatcgatg cgctgcagcg ccccggctac
     1321 cgaaagcgaa cttttgactt cgtcgttctt ctgcttgtga atgatctcgg tcagcgacca
     1381 taaaattccc aggccgaaga gaattcccat gtacggtgga agaagggtaa cgcttttgaa
     1441 aacgggaaca agcagcagca cggctgcgcc ggtgaataaa acgaggtttt gctcgaaacg
     1501 cgttgtggga cgtgaatagg ttttttcatt ttccggcggc tcgatgttcc cgctcatttt
     1561 ccggcgaagc cacaaatacg gcaggaaaag gctgacgagc gcgggaacaa aaagcttcag
     1621 gatgatattg aggctcgaga tctgtccgcc gatccacagc atcgttgtcg tcacgtcgcc
     1681 caccggcgac cacacgccgc ctgcgtttgc cgcgatgatg atcatcccgc cgtaatacat
     1741 gcggtcggtt ggatctttca ccagtttgcg caccagcgac atcatcacca gcgccgtggt
     1801 gaggttgttg agtaccgacg aaagcaggaa cgtgatgagg ctgacgagga acagcagttt
     1861 ccgtttgctt ttcgtgcgca gcttttccgt gatgagcgtg aaaccgtcat gcgcatcgat
     1921 cagctcgacg atcgccatcg cgccgagaag gaaaaaaagt atttccgaaa tgcccgaaag
     1981 ctgcacgcca agcttcgaat cgatcagtcc cgcgttggtc ccggcgatca cgatcatcca
     2041 gcagaccacg ccggtgagca gcgcagaagc ggacttgttg accttcagcg tttgctcgaa
     2101 agcgatcgcg agataaccga ggatgaaaac ggaagcgaga agcatcggac ggtaaaaata
     2161 ctctgttctg cgtttgcgcc gtgaagcaac ggccggatat gaagtgcggg ttccccttat
     2221 ttcaattctt ggttatattt ggcaaatcat aaacggaata aaacccaaat cacgctatga
     2281 gcattttccg gaagaaatca cttgaaacgt tgctggcaca ggcggcggaa tctgaaaaag
     2341 gactgaagcg tacgctggga tcgacgcagc tggtcgcgct cggcatcggg gcgatcatag
     2401 gcgcaggcct tttcgtccgc actgcagcag ctgcggggga agcggccgga cctggcgtca
     2461 ctctttcatt cattgttgca gcgatcggct gcgcgttcgc cgggttgtgc tacgcagaat
     2521 tctcggcgat gatcccgatt gcgggaagcg catatacata cgcctacgca acaatgggtg
     2581 agcttatcgc atggatcatc gggtgggcgc tcattctcga atatgcgctt ggggccgcga
     2641 cggtttccat tgcatggagc gaatacctga acaagctgct tggcggacac attcctttcc
     2701 agtggtgcca cggcccgttt gaaagcgcga ctgtcgacgg aaccctggtt cacgggattg
     2761 tgaatgcgcc tgcgctgttt atcctgctcg ccttgtcgct tctgctcatc cgcgggacgc
     2821 aggaatcagc ttttgtcaat gcgatcatcg tgtttctcaa agtggcaatc gtacttgtgt
     2881 tcatcgcgat cggctggcag ttcatcaagc cggaaaatca tacgccgtat ctcattcctg
     2941 ccgattaccc gggtcacgcg gggattttcc agcatggctg gggaggcata ctgaaaggcg
     3001 cgggcatagt tttcttcgcg ttcatcgggt ttgacgccgt ttcaaccgcc gcgcaggaag
     3061 cgaaaaatcc gaaacgcgac atgcccatcg gcatcctcgg ctcgctggtg gtatgcacga
     3121 tcctctacat cctgttctcg catgttctta ccggtgttgc accctgggcc gatttcaacg
     3181 atcccgcgaa aggcaaggaa gcttctgttg cgtacgccat ctccacctac atgcagggtt
     3241 acggatggct tgccacggcg gtaacgatcg ctattctcgc cggattttct tcggtcattc
     3301 tcgttatgct catgggccag agccgtgttt tctattccat gtcgcacgat ggactgctgc
     3361 cgaaaatatt ttctcatctc cacccgaaat tccgcacacc gtataaatcc aatatcctgc
     3421 tcttctgttt cgtcgggctt ttcggcggat tcattcccgg cagtgtcgcc ggcgacctga
     3481 ccagcatcgg cacgctgctc gcattcgtgc ttgtatgtgc gggtatctgg atcatgcgtg
     3541 ttaattcacc cggagctgtg cggcccttca ggacgccggg agcatcgccg tccttcccga
     3601 tcgttcccat tcttggaata gtcgtgtgca gcttcatgat catttctctc gacaggttga
     3661 cgcagatcag cgcgttcggg tggatgatct tcgggctgat tatttatttc cttcacggga
     3721 aaaagaacag taagctgaac gtgcttcagt aaattcagcg ccattcgctg aaccgcaatc
     3781 gtaactggtt gcggtttttc atttgtcatg gaagaaaaaa gagagttcag gcgttcgctc
     3841 ggtctcattg acgccacggc gctcgtcgcc ggatcgatga tcggttcggg aatttttatt
     3901 gtcagcagca cgatggcgca ggatctcggc tgcgcaggct ggctcatcgt cgcctggatc
     3961 atgacgggcg tgatcacgct caccgccgcg ctcagctacg gcgagctggc cggcatgatg
     4021 ccggaagccg ggggacaata cgtttacatc cgccgcgcgt ggggcgagct gcccgcattt
     4081 ctctacggct ggactgtttt ttccgtcata cagaccggcg ttatcgcagc tgtggcgacg
     4141 gcatttgcga attacacttc ggagctcgtc ccggcgctga gtatgaagaa tgtgctgctg
     4201 gacgccggtt tcattaaaat ttcttcgggg caggttttcg ccgttgcgat gatctttttt
     4261 ctttcgtgga tcaattcccg cggcgtgcag ggcggcgcgc ttgtgcagaa agtatttact
     4321 tccgcaaagc tgatcgcgct tttcggactg atcattctcg gtatttccat cgggctgaag
     4381 agcgggaacc tcgcgcacaa ttttaccgat ccctggatgg ctacacgcat ccgcgtttcc
     4441 gacagcggct cttacagcct gacaccgctc accggttttg cgctgctcgc cgtgttcggg
     4501 atgacgatga tcaattcgct tttttctgcc gatgcctgga acaacgtcac gttcattgcc
     4561 ggcgagatcc gcgagccgca caaaaatatt ccgcgaagcc tgctgctcgg caccgccatc
     4621 gttacaggtc tttatgttct cgcgaacatc gcttacctcg cgctgctccc gctgaaaggc
     4681 gacccgggcg gaaccgacgt gatgtcgcgc ggcatgcagt ttgccgaaat gggccgcgtg
     4741 ggaacagccg ccgcaacgat gatcttcgga aagatcgcca cgatcatgat ggcggtcctg
     4801 atcatggtgt cgacgttcgg ctgcaacaac ggcattattc tcgccggcgg acgactgtac
     4861 tacgcgatgg cgaaagacgg cctgttcttc gaccgcatcg cgaagctcaa caggaaaaat
     4921 gtcccggcgt ccgcgatctg gatacaggcc gtgtggggaa gcctgctctg cttttccggg
     4981 acatacaacg atctgcttac gtacgcgaca ttcgcctcga tgctgtttta catcgttacg
     5041 atctcaggtc ttttccgttt acgcaaaata gaaccgggca caccgcggcc ctataaagcg
     5101 cttggctatc ccctgcttcc tgcgttttat attctcgctg ccgccggcat ctgcatcgat
     5161 ctgctcatca tggatccgcg gaacagctgg gccggcctcg gtatcgttgc cctgggaatt
     5221 cccgttttct tcatccggaa agcatttcct tcacaaaaaa cggcagtgta aattattccg
     5281 gttgttttcc gaagtgtttt tttaccaaaa tttgccagcc ggagaaattc ttccgcatgc
     5341 gcaactggtt ccgaaataca ttttcattta cacgccgcga acggaacggc gctgccgtcc
     5401 tcatcgtaat cctcattgcg ctcagcgcat tcacggctgt cagctccagg tttgtttcac
     5461 gtccacagga agatttttcc gggttcgata aattcatcgc acaggtgaaa caggattccc
     5521 tgttcgattc cctggccgga aaaaacacgg aaaacaaacc cccgtccatt gccgaagaag
     5581 aagcggatca tgtatttcac cagcgggaac ttttccagtt cgatcccaac gggcttcctg
     5641 tcagcgactg ggttcgtctt ggtctcacgc ccgcgcaggc acgttcggtg aaacattacg
     5701 aagaatccgg cggaaaattc gaatcgaagg aagatgtgaa acggctctat gtcgtttccc
     5761 cgaaattgta cgcagacctt gagccctata tcctgcttcc ggataaaaaa gagaacgacg
     5821 cacgcccgga taaatttcct gcgaagaaaa atcccccggc cattctcgag ctgaacacgg
     5881 ctgattcggc tttgctcgtt acggtaaatg gtatcgggcc ggtgtttgca aaacgcatta
     5941 tcgaatacag ggagaagctg ggcggttttt att
//
LOCUS       DASTSF010000109         4139 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1494586,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000109 DASTSF010000000
VERSION     DASTSF010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4139
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1494586"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="VFU15_03290"
     CDS             <1..270
                     /locus_tag="VFU15_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020212825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase
                     family protein"
                     /protein_id="HEU4716826.1"
                     /translation="NEEYLEACRRGDIVEIADALGDLLYITFGTILRHGLQYKIEEVF
                     DEIHRSNMSKLDESGKPVFREDGKVMKSERYFRPDIKSILEKERK"
     gene            complement(353..1006)
                     /locus_tag="VFU15_03295"
     CDS             complement(353..1006)
                     /locus_tag="VFU15_03295"
                     /inference="COORDINATES: protein motif:HMM:NF013933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HEU4716827.1"
                     /translation="MTTFSAIGQGMLFGLALCFTLSPSFFTLIQTTLKNGHAAGISVA
                     TGIFFADLCYLLLAYFGVSEWVMDERYAIAITVLGGGLLIGYGIVQILKKPVVSNENE
                     ESDSPAGKKAGIGANILKGFLMSLLNPFVFFLWIGALSLATSRFDHDAGKMISFFIAT
                     LCTVFGTDVLKSMAAHRIKSFLNEKMIHKVNMFAGIIFVIAGIVMFVRMIVEKPWLG"
     gene            complement(1003..2550)
                     /locus_tag="VFU15_03300"
     CDS             complement(1003..2550)
                     /locus_tag="VFU15_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008201325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HEU4716828.1"
                     /translation="MALLGSILKRTIDLNTKIPRLRRKDAMKMQVKTLKKLLSKAEFT
                     AFGEHHNFTRILSESDTVKSFKAAVGVHDYNSMFRKWWYRSLNGEAYVTWPEKVKYFA
                     LSSGTSDSSSKHIPVTKDMLRAIRRASRRQLLALAHYNLSPEFFQKGILMVGGSTHLN
                     YNGSYYSGDLSGITTGNLPFWFQHFYKPGRRIARERKWEDKLQEMVMKAKEWDIGVVC
                     GVPAWIQILFERIIKQYNVKTIHDIWPNLEVYVWGGVSIKPYRKSLDKLCGKPIGYLE
                     TYLASEGFMAFQNDPTSESQVMQLDNGIFFEFVPFNSDNFDADGNLKPKPSTLTIAEV
                     DEQTEYALLISTCSGAWRYLIGDTVKFTNVDKAEIIITGRTKQFLSLCGEHLSQDNMN
                     RAIDLAAEELNIEINEYTVAGENYENLFAHRWYVGTNAKIDPEVLRKTIDEHLKVLND
                     DYRVERSAALKEVIVEVLPLDFFSEWMRRNGKVGGQHKFPRVMKNDRLESWRKHIAEM
                     KSGELAH"
     gene            complement(2581..3438)
                     /locus_tag="VFU15_03305"
     CDS             complement(2581..3438)
                     /locus_tag="VFU15_03305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716829.1"
                     /translation="MKKEKRFLWFPLAATLTLISCGGEKPSDNHPDTLHDTLITTNTE
                     DSVHYLFKRMVATLPVPFEIMKKFEGANLPFNGSVLCNPDNAGKFNSASAQAINLGVF
                     GADMSYLISQNHLGESAPYLKGVRKLSDNIVVPSAFDEGLIGRYESNMSQKDSLQRLM
                     HTSYKRIDSTLQENDRLALASLVLAGGWLESIYITTQHIAEQQQNEKNKVLYDMLAIQ
                     NPYVTRLSDLLGRFTSDSLCISLHRDMEDLKKVTPPGPDIPPDQFANQLTMFRTKIAD
                     IRNKYVRVQ"
     gene            complement(3442..>4139)
                     /locus_tag="VFU15_03310"
     CDS             complement(3442..>4139)
                     /locus_tag="VFU15_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013632065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M42 family peptidase"
                     /protein_id="HEU4716830.1"
                     /translation="EETPSLKNIFLDCGCSSDKEVAALGVHVGCVVTYEDPFMILNDR
                     YYVGRALDNRIGGFMIAQVARLLKANRVKLPYSLYIVNSVQEEVGLRGAQMITETIRP
                     NVAIITDVTHDTQTPMMNKIAQGDIACGKGPSLTYGPAVHNKLLAQIIGVAEKNRIPF
                     QREAVSRSTGTDTDAFAYSLGGVASALISLPLRYMHTTVEGVHKNDVDSVIRLILESL
                     KSIKAGQDFRYIR"
BASE COUNT          913 a         1078 c         1139 g         1009 t
ORIGIN      
        1 aacgaggaat acctcgaagc ctgccggcgg ggcgacatcg tggaaattgc cgatgcgctc
       61 ggcgacctgc tctatatcac tttcggcacg atcctccggc acggcctgca gtacaagatc
      121 gaagaagtgt tcgacgagat ccaccgcagc aacatgtcga agctcgacga gagcgggaaa
      181 cctgttttca gggaagacgg caaagtgatg aaaagcgaaa ggtatttcag accggatatc
      241 aaaagcattc ttgaaaagga aaggaaataa agtccggatg ctacaggtga aaatggatat
      301 tcaaatccga catctgatat tcgaaatccg acatccaaaa tccgacatcc tactacccca
      361 gccagggttt ttccacgatc atacgcacga acatgacgat gccggcgatc acgaaaatga
      421 tccctgcaaa catgttcacc ttgtggatca tcttttcatt caggaaactt ttgatgcggt
      481 gcgccgccat tgacttgagc acgtcggttc cgaaaacagt gcaaagcgtg gcgataaaaa
      541 agctgatcat ttttcccgca tcgtgatcga agcggctcgt ggccagcgac agcgcgccga
      601 tccacaggaa aaaaacgaac gggttgagca ggctcatcag gaaacctttc aggatattcg
      661 cgccgatgcc ggcctttttt cccgcggggc tgtcgctttc ctcattttca ttggaaacaa
      721 ccggcttctt cagtatctgc acaatgccat agccgatcag cagtccgccg ccaagcacag
      781 tgatcgcgat ggcgtaacgt tcatccatca cccattcgga aacgccgaaa taggcgagaa
      841 gaaggtagca gagatcggcg aaaaaaattc ctgttgcgac ggagatcccg gccgcgtgcc
      901 cgttcttcag cgttgtctgg ataagggtaa aaaaggaagg actgagcgta aagcaaagtg
      961 ccagcccgaa aagcatccct tgtcctatgg cgctgaacgt ggtcaatgtg caagctcccc
     1021 tgatttcatt tctgcaatgt gcttcctcca gctttcgagg cgatcgttct tcatcacgcg
     1081 cggaaattta tgctgtccgc ccacttttcc gttcctccgc atccattcgc tgaaaaaatc
     1141 cagcggcagg acttcgacga tcacttcttt caatgcggcg gaacgttcca cgcggtagtc
     1201 gtcgttcagc actttgaggt gctcgtcgat cgttttgcgc agcacttcgg gatcgatctt
     1261 cgcgttggtt cccacgtacc agcggtgcgc gaaaagattt tcgtagttct cgcccgccac
     1321 cgtgtattcg ttgatctcga tgttcagctc ttccgcggca agatcgatgg cgcggttcat
     1381 gttgtcctgc gaaagatgct ccccgcagag gctgaggaat tgctttgtgc ggccggtgat
     1441 gatgatctcc gctttgtcga cgttcgtgaa ttttaccgtg tcgccgatga ggtaacgcca
     1501 ggccccgctg caggtgctga tgagcagcgc gtattccgtc tgctcgtcca cttccgcaat
     1561 ggtgagcgtg gacggtttcg gtttcaggtt gccgtccgca tcgaaattgt cgctgttgaa
     1621 cggaacgaat tcgaaaaaaa ttccgttgtc gagctgcatc acctggcttt ccgacgtcgg
     1681 atcgttctgg aatgccatga aaccttccga agcgagataa gtttcgaggt aaccgatggg
     1741 ttttccgcaa agcttgtcga ggcttttgcg gtacggtttt atggaaacgc cgccccacac
     1801 gtacacttcg aggttcggcc agatatcatg tatcgttttg acgttgtatt gcttgatgat
     1861 gcgttcgaaa aggatctgta tccacgcagg aacgccgcac accacgccga tatcccattc
     1921 cttcgctttc atcaccattt cctggagctt gtcttcccat ttgcgttcgc gcgcgatgcg
     1981 tcttcccggt ttgtaaaaat gctggaacca gaaggggagg ttcccggtcg tgatgccgga
     2041 aaggtcgccg gaataatagc tgccgttgta attgaggtgc gtgcttcccc cgaccatgag
     2101 gatccctttc tggaaaaatt cgggcgagag gttgtagtgc gcgagcgcaa gcagctggcg
     2161 gcggctggcg cgccggatcg cgcgaagcat atctttcgta acgggaatat gcttgctcga
     2221 tgaatcggac gtgccggagc tgagcgcaaa atatttcact ttttccggcc aggtgacata
     2281 agcttccccg ttcagggaac ggtaccacca tttgcggaac atggaattgt aatcatgaac
     2341 accgaccgcg gccttgaagg atttcacggt gtcgctttcg ctgagtatgc gcgtgaaatt
     2401 gtggtgttcc ccgaatgcag tgaactctgc tttcgaaagc agctttttca gtgtcttcac
     2461 ctgcatcttc atcgcatcct tcctgcgcag gcgtggaatt ttcgtgttga ggtcgattgt
     2521 acgtttcaga attgatccaa gtagtgccat gaccgggaga gataacgacg taaaaatagt
     2581 ttactggaca cgaacgtatt tgttccggat gtccgcgatt tttgtgcgga acatcgtcag
     2641 ctgattcgca aactggtcgg gtggaatgtc aggtcccggc ggcgtgacct tcttcagatc
     2701 ctccatgtcg cggtgaagcg aaatgcagag gctgtcggaa gtaaatcttc ccagcaggtc
     2761 ggagagccgc gtgacgtatg gattctggat cgcgagcatg tcataaagaa ctttgttctt
     2821 ttcattctgc tgctgctcgg cgatgtgctg cgtggtgatg taaatgcttt cgagccatcc
     2881 cccggcaagc accagcgagg cgagcgcgag cctgtcgttc tcctgcagcg tactgtcgat
     2941 gcgtttgtag gaagtgtgca tcaggcgctg cagcgaatct ttctggctca tattgctttc
     3001 ataacgcccg atcagtccct cgtcgaaggc ggaagggact acgatattgt cggaaagctt
     3061 ccgcacgcct ttcaggtatg gagccgattc gcccaggtga ttctgcgaga tcaggtacga
     3121 catgtctgcg ccgaaaacgc cgaggttgat cgcctgcgcc gaagcgctgt tgaatttccc
     3181 ggcattgtcg ggattgcaga gaacgctgcc gttaaaagga agattggctc cctcgaattt
     3241 tttcatgatc tcgaacggaa caggaagcgt ggccaccatc cgtttgaaaa gataatgcac
     3301 cgaatcctcg gtgttcgttg tgatcagcgt atcgtgaagc gtatcaggat gattgtcgga
     3361 cggtttttct ccgccgcagg agatgagcgt cagcgttgcc gccaggggaa accacagaaa
     3421 acgcttttct tttttcatcg ttcagcggat gtaacggaaa tcctggccgg ctttgatgct
     3481 tttcagcgat tcgagaatga ggcggataac gctgtccacg tcgtttttgt gaacgccttc
     3541 caccgtcgta tgcatgtaac ggagcggaag cgaaataagc gccgaggcca cgccgccgag
     3601 cgagtaggcg aacgcgtcgg tatccgttcc cgtgctgcgt gaaacggctt cgcgctggaa
     3661 aggaatgcgg tttttttccg ccacgccgat gatctgcgca aggagcttgt tgtgaacagc
     3721 cggaccatac gtaagcgaag gtccttttcc gcaggcgata tcaccctgtg cgatcttgtt
     3781 catcatcggc gtctgcgtgt cgtgcgtgac gtcggtgatg atcgccacgt tcgggcggat
     3841 cgtttcggtg atcatctgcg cgccgcggag gccgacttct tcctgcacgg aattcacgat
     3901 atacagcgag tacggaagct tgacgcggtt cgctttcagc aggcgcgcca cctgtgcgat
     3961 cataaagccg ccgatgcggt tgtccagtgc gcgaccgacg taataacggt cgttgaggat
     4021 catgaacgga tcttcatacg tcaccacgca tcccacgtgc acgccgagcg cggctacttc
     4081 cttgtcggac gagcatccgc agtcgagaaa aatatttttc agcgaaggcg tttcttcct
//
LOCUS       DASTSF010000110         3130 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1940576,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000110 DASTSF010000000
VERSION     DASTSF010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3130
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1940576"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..296
                     /locus_tag="VFU15_03315"
     CDS             <1..296
                     /locus_tag="VFU15_03315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716831.1"
                     /translation="QQGKQELIMVHDAIRFDDDRAAEWLHEYGHDDWGLFLTAIGGDP
                     YAVQALFDGKQARLAMAAGAIIGDQRSMEYLQKHGLKAWLHLVEAVRNIQSEE"
     gene            368..787
                     /locus_tag="VFU15_03320"
     CDS             368..787
                     /locus_tag="VFU15_03320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716832.1"
                     /translation="MAQKIYNGEAIRKMTALFKPFSTPAYNWLMEKGHRELIEAAEFL
                     KDGNQLHFRWLVDNKHFEMGAFINAVKGDKDAFRWLMQNKSIFWAATANAINKDREAK
                     AWLKANNFIVYAELADAIVEFEKGNNDDIAGYYRPPI"
     gene            897..3098
                     /locus_tag="VFU15_03325"
     CDS             897..3098
                     /locus_tag="VFU15_03325"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU4716833.1"
                     /translation="MRQSELYHPKKIRIQLGVAVALSLMLVGLLGWFSRKNNADLANA
                     LGEQEKGQRVIEKISEFRDSLIVTHDQLLLFNERGEASVLDNLQLSTLHFRQLMKELD
                     GLVTDPSLKPQLDRLHQLADEKIQHDRVIVRMRISGAEVSTADLSESEALTDELFTAS
                     KTLEKDHKKLLLSRINESAALSSRITFFDTVAAVLAVLIICMAGILLFRDISKRNKLE
                     KELLAATERVHQSAVLKEQFMANMSHEIRTPLNAVLGFAELLARTNLDDQQKEHLRVI
                     RQSGQNLLHILNDILDFSKLEAGMMRLEEIPFSVQGLMHSISTMFQHRATEKRIKLVF
                     HPIENIPDPLTGDPTRLTQILVNLVGNAIKFTDEGYVEVRTRLLKEENGRLLVQFSVH
                     DTGIGIPSERMNDIFDRFNQGENDTARKYGGTGLGLSIVKRLVELQGGIISVDSVPGK
                     GTTFTFLLPYTRYAAALPAETVSRKEEPGFIPEFFSGISVLVAEDNPLNQRLIGELLS
                     SWKISFDLASTGPEVIGLLRKKTYDLVLMDIQMPGQNGYETTRQIREELKLSLPIVAV
                     TAHAMPGEREKCISSGMNDYLAKPISEREFFHLLRRILSGKPQLLKTVYRKDDGGSER
                     ITNLDYLHELANGNEGFVAEMISLFLSENPVELETMERAIDEMDFAMIVKTAHKMRST
                     IPFVGLSQKLDQPLKNMEVIAASTQNISELKQLFSFVSVTCRKAAAELAAA"
BASE COUNT          762 a          878 c          812 g          678 t
ORIGIN      
        1 agcagcaggg caagcaggag cttatcatgg tgcatgatgc gatccgtttc gatgacgacc
       61 gtgcggcgga atggctgcat gaatacggtc atgacgactg gggccttttc ctcaccgcga
      121 tcggcgggga tccgtatgcc gtacaggcat tatttgacgg aaagcaggcg cgcctggcca
      181 tggctgcggg cgcgatcatc ggcgaccagc gatcgatgga atacctgcag aagcacgggc
      241 tcaaagcgtg gctccatctt gtggaagccg tgcgcaatat ccagtcggaa gaataagtta
      301 agttttacaa acggcccctt ccatttcctt tttcccttac ggaatctgtt atttttgtat
      361 tccctgtatg gcgcagaaaa tatataacgg tgaagcgatc cgcaaaatga ccgcattatt
      421 caagccgttc agcactccgg cctacaactg gctgatggaa aaaggtcacc gcgagctgat
      481 cgaggcggcc gaattcctga aagacggcaa ccagcttcat ttccgctggc tcgtcgataa
      541 caagcatttc gagatgggcg cattcattaa tgcggtgaag ggagataaag atgctttccg
      601 ttggcttatg cagaataaaa gtattttctg ggcggccacg gccaatgcca tcaacaaaga
      661 ccgcgaagcc aaagcctggc tcaaagccaa taattttatc gtttacgcag aacttgccga
      721 cgcgatcgtc gaattcgaaa aaggaaacaa cgacgatatc gccggttatt accgcccccc
      781 gatctgagga atcccggcta taacgcctcg cttttcagcc tttattcgtc ccacctcagc
      841 gaaaattttt catgaacgcc ccgccagaag ataaattact gctaaagaaa aaagtcttga
      901 ggcaatcgga gctttatcat ccgaaaaaga tcaggataca gctgggagtg gcggttgcac
      961 tttccctcat gctggtcggt ctgctcggtt ggttttcacg gaaaaacaac gccgatctcg
     1021 caaacgcgct cggtgagcag gaaaagggac agcgcgtgat cgagaagatc agcgagttcc
     1081 gcgattcgct catcgttacg cacgaccagc ttcttctttt caacgagcgg ggcgaagctt
     1141 ccgtgctgga taacctccag ctgtccacgc ttcatttccg ccagctcatg aaagagctcg
     1201 acgggctggt gaccgacccg agcctgaagc cgcagctcga ccggcttcac cagctggccg
     1261 atgaaaagat ccagcacgat cgcgtgatcg tccgcatgcg catctccggc gcagaagttt
     1321 ctacagccga tctttccgaa agcgaagcgc tgaccgatga gctcttcacc gcttccaaaa
     1381 cgctcgagaa ggatcacaag aaactgctgc tgtcgcgcat caatgaaagc gccgcacttt
     1441 cttcgcgcat cacctttttc gatacggtcg cagcggtact tgccgtgctg atcatctgca
     1501 tggccggcat tcttcttttc cgtgatattt ccaagcgcaa caagctggag aaagagcttc
     1561 tggccgcaac ggaacgcgtg caccagtcgg ctgtgctgaa agaacagttc atggcgaaca
     1621 tgagccacga gatccgcaca ccgctcaacg ccgttctcgg ttttgccgag ctccttgcac
     1681 gcaccaacct tgatgatcag cagaaggagc acctgcgcgt catccgccag tcgggacaaa
     1741 acctgctgca tatcctcaat gatattctcg atttttcgaa gctcgaagcc ggcatgatgc
     1801 gtctcgagga aattccgttc agcgtgcagg gactcatgca ttccatcagc acgatgttcc
     1861 agcaccgcgc aacggaaaaa aggatcaagc tggtatttca cccgatcgaa aatattccgg
     1921 acccgctcac aggcgatccc acgcgcctga cgcagatact cgtcaacctc gtcgggaacg
     1981 cgatcaagtt caccgatgaa ggttacgtgg aagtccgcac gcgtttgctg aaagaagaga
     2041 acggacgtct cctcgtgcag ttttccgtgc atgatacggg catcggcata cccagcgaaa
     2101 ggatgaacga tatttttgac cgcttcaacc agggggaaaa cgacacggcc cgcaaatacg
     2161 gcggaaccgg tctcggtctt tcgatcgtca agcggcttgt cgagctgcag ggcggcatca
     2221 tcagtgttga cagtgttccc gggaaaggaa cgacgttcac attccttctg ccctacaccc
     2281 gttatgctgc ggcgcttcct gcggaaactg tttcgcggaa agaagagccc gggttcattc
     2341 ccgaattttt ctccggcatt tccgttcttg tcgccgagga caacccgctc aaccagcgtc
     2401 tcatcggcga gctgctgtcc tcctggaaga tcagctttga ccttgcatcc accgggcctg
     2461 aagtgatcgg gctgctgcgt aaaaaaacat acgatctcgt gctgatggat atccagatgc
     2521 ccgggcagaa cggttatgaa acgacgcggc agatccgcga agagctgaag ctttccctcc
     2581 cgatcgtcgc tgttacggcg cacgccatgc ccggcgagcg ggaaaaatgc atttcctccg
     2641 ggatgaatga ttatctcgcc aagccgatat ccgagcgcga atttttccac ctgcttcgcc
     2701 gcatactttc cgggaagcca caactgctga aaaccgttta caggaaagat gacggcggct
     2761 ccgaacgtat caccaacctc gattacctgc atgagctggc gaatggaaat gaaggattcg
     2821 ttgcagaaat gatcagcctt ttcctttcgg agaacccggt cgagctggaa acgatggaac
     2881 gcgccatcga tgaaatggat ttcgcgatga tcgtgaagac ggcgcacaaa atgcgatcga
     2941 ccatcccgtt tgtcgggctt tcgcagaagc tcgatcagcc gctgaaaaac atggaggtca
     3001 tcgctgcctc cacgcagaac atcagcgagc tgaagcagct gttctcgttt gtttccgtta
     3061 cctgccgcaa agccgctgca gaacttgccg ccgcctgatc atctccaatg cgaatcatcg
     3121 gaaacatacc
//
LOCUS       DASTSF010000111         3189 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_597757,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000111 DASTSF010000000
VERSION     DASTSF010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3189
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_597757"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..188
                     /locus_tag="VFU15_03330"
     CDS             <1..188
                     /locus_tag="VFU15_03330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716834.1"
                     /translation="SSTDRRKSSVTVTPLGKKMLAQCSAGAEKFRKNAGKGISQEELN
                     HLKATLDRLYGNCGGAQ"
     gene            complement(182..1021)
                     /locus_tag="VFU15_03335"
     CDS             complement(182..1021)
                     /locus_tag="VFU15_03335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716835.1"
                     /translation="MLPMKTFLKISFPLLLLLIVTGFSFPHESQPGKTNDAGNDTSGL
                     RIIHVFVSLCDNDSQGIVPVARKNGKGNDGPDNLFWGCANGVKTFFLESDDWDLLDSV
                     KEPTHEVLERCVWKSRRYNAILVAEGYRGGRIRNCTMDFLRTASGNCTDSITVNDHGK
                     KIVVHPGDAQLVCYLGHDGLMDFDITDPPKKNGASRDVIIISETGRLYFKDVIRAGGG
                     NPLLWTTNLIVPGASTLKAAIDGWLMKETGEQIRQRAAKAYDAEQKCGADAALALFAT
                     GFY"
     gene            1144..1398
                     /locus_tag="VFU15_03340"
     CDS             1144..1398
                     /locus_tag="VFU15_03340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716836.1"
                     /translation="MFFYPHFSLTQKTILHLVLAVASVVFGWLALQYYKKAIPTGSNL
                     GCLYYFFLLLNLVGGVGAVFFGLVQVFSAEGGSSETLWLL"
     gene            1395..2063
                     /locus_tag="VFU15_03345"
     CDS             1395..2063
                     /locus_tag="VFU15_03345"
                     /inference="COORDINATES: protein motif:HMM:NF013713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716837.1"
                     /translation="MKKWMSWIWPRVIKRTRGKISPVLEVSWENGRKVLNARYVNYSY
                     GSLHDVFRIALRNVVDLSDPPRNVLILGFGVGSIASILTEEMKISCTLTGVEADEEVI
                     RLGKEEFDTGRFSGLRIVHLRAENFVRQCSEEFDLIAIDVFVEDKVPRSLMNEEFLLR
                     VEKLLSAKGRVVMNIMENDSSGGGGFRRLFQSVFSSPQANDLDMGVSVNRLLTGSKKK
                     KSIS"
     gene            complement(2053..>3189)
                     /locus_tag="VFU15_03350"
     CDS             complement(2053..>3189)
                     /locus_tag="VFU15_03350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716838.1"
                     /translation="LQGFSRDPRCKAGINDGTGTGSSETSATKLPIGLPFTFAENGSA
                     SGSSSSAPASDSKKIVYTFDMPSDIGPEALHATHSAFQQARTMNASCVLIRMNSYSGA
                     LDAAENIRQEILDYDRPVMIYVENREMTASNLISTACDSVYLRHAPKVPRATAPSRNA
                     VASPAGDDKDPATDLEQETHSTASLRSLPLDQECRPASKLRGEASDINDVLLHAGLRD
                     CKVVHYNPGFLSKAIDFLMRRPMSLLLVVLLAFGLSLQRKESLPGPSTFIVLVSSGFF
                     VLPLFMGGLATGKEIALFAASVLATILLNRGRQKLWLRLLPVAALLLLLTLCQYTRLS
                     FSYDSDLTVLLLLTSLSFFAGWNIPVMLRFFPGRQVPVPVRIRK"
BASE COUNT          729 a          818 c          930 g          712 t
ORIGIN      
        1 ggagcagcac ggaccggcga aaatcgtccg tcaccgttac tcctctcggg aaaaagatgc
       61 ttgcgcagtg ttccgcaggc gcggagaaat tccggaaaaa tgcaggcaaa ggaatctcgc
      121 aggaagagct caatcacctc aaagcaacgc tcgacaggct gtatgggaac tgcggcggag
      181 ctcagtaaaa acctgtcgcg aaaagtgcaa gcgcggcatc cgcaccgcat ttctgttctg
      241 catcgtaggc ttttgccgca cgctgcctga tctgctcgcc cgtctctttc atcagccatc
      301 cgtcgatagc ggctttcagc gtggatgcgc ccggtacgat gaggtttgtt gtccacagca
      361 gcggatttcc gccaccggcg cggatcacgt ccttgaaata caggcgcccg gtttccgaaa
      421 tgatgatcac atcgcgcgaa gctccgttct ttttcggcgg atccgtgatg tcgaaatcca
      481 tcagcccgtc gtgaccgaga tagcagacga gctgcgcatc accgggatga acgactattt
      541 tttttccgtg atcattgacg gtgatcgaat cggtgcaatt gcccgatgcg gttcgtagaa
      601 aatccatcgt gcagttgcga atccgcccgc cgcgataacc ttcggctacc aggatcgcat
      661 tataacgccg gctcttccag acgcagcgct cgagaacctc atgcgtgggc tctttcacgg
      721 aatcgagcag gtcccagtcg tcgctttcca ggaaaaacgt tttgacgccg ttcgcgcagc
      781 cccagaacag gttgtcgggt ccgtcgtttc cttttccgtt cttcctggca acaggaacaa
      841 tgccctggct gtcattatcg cagagcgaaa cgaaaacatg aataatgcgc aggccggagg
      901 tgtcgttccc ggcatcattc gttttccccg gctgagattc gtgcgggaaa gaaaaaccgg
      961 tcacgatcag caggaggaga agggggaaac ttattttcag gaacgtcttc atgggaagca
     1021 cgcgtacgtt aattttgcag acaccaacga atttacaaaa tgcaggccac gaacaggttg
     1081 agattaaact cggtattcag cttttttttc tccttcgccc tgcgggcgat gtacaatgcg
     1141 ttcgtgttct tctaccctca tttttcattg acgcaaaaga cgatcctgca tctcgttctt
     1201 gccgtggcga gcgttgtatt cggatggctg gcgctgcagt attacaaaaa agcgattccc
     1261 acaggttcca acctcggctg cctttactat ttcttcctcc tgcttaatct tgtcggcggc
     1321 gtcggcgctg tttttttcgg actggtccag gttttcagcg cggaaggcgg ttcgtcggaa
     1381 actctctggc tgttatgaaa aaatggatga gctggatatg gccgcgcgtg ataaaacgca
     1441 cgcggggaaa gatatctccc gtgcttgaag tgtcgtggga gaacgggaga aaagtgctga
     1501 acgcgcgtta cgtgaattat tcctacggct ctcttcatga tgttttccgc atcgcgcttc
     1561 gcaacgtggt cgacctttcc gatcctccgc gcaatgtgct gatcctcggt ttcggcgtgg
     1621 gaagcattgc atccattctt acggaagaaa tgaaaataag ctgcaccctg acgggcgtgg
     1681 aagccgacga agaagtgatc cggctgggaa aggaagaatt cgataccggg cgcttctccg
     1741 gtctgcggat cgttcacctg agggcggaaa attttgtcag gcaatgcagc gaagaattcg
     1801 atctcatcgc tatcgatgtt ttcgtggagg ataaagttcc gcgatcgctc atgaacgaag
     1861 aattccttct ccgtgttgag aagctgcttt ccgcgaaggg gcgggtggtg atgaatatta
     1921 tggaaaatga ttcgtcggga ggcggcgggt tccgcaggct tttccagtcc gttttttcgt
     1981 caccgcaggc gaatgatctc gacatgggtg tgtcggtgaa caggttactt acgggaagca
     2041 aaaagaaaaa atctatttcc tgatcctgac gggaacagga acctgcctgc cggggaaaaa
     2101 gcgaagcatc acgggaatgt tccacccggc gaagaaggaa agtgaggtga gcagcaggag
     2161 gacggtaaga tcactgtcat aggaaaacga caggcgcgtg tactggcata gcgtcagcag
     2221 cagcagcaat gccgcgaccg gcagcaggcg aagccatagt ttttgtctcc cgcggttcag
     2281 gagaatcgtg gccagtaccg atgcggcgaa aagggcgatc tcttttcccg tagccagtcc
     2341 gcccataaaa agcggcagca cgaaaaaccc ggaagaaaca agaacgatga acgtggaagg
     2401 tccgggcaat gattctttcc gctgcaggct gaggccgaac gcaagcagga caacaagcag
     2461 gaggctcatg ggccggcgca tgagaaaatc gatagccttg ctcaggaacc cgggattgta
     2521 atgcaccacc ttgcaatccc tgaggccggc atgaagaaga acatcgttga tgtcgctggc
     2581 ttcgccgcgg agcttggacg caggcctgca ttcctggtcg agaggcagcg aacgtaaaga
     2641 agccgtgctg tgcgtttcct gttccaaatc ggtcgcggga tctttatcat cgcctgccgg
     2701 tgacgcaaca gcattcctgg aaggcgctgt cgcgcggggg actttcggag catgacgaag
     2761 gtaaacgctg tcgcaggcgg tggaaataag attcgaggcc gtcatttccc ggttctcgac
     2821 gtagatcatc accgggcggt cgtaatcgag tatttcctgc cggatgtttt ccgcggcatc
     2881 cagcgcaccg gaatagctgt tcatccggat caggacgcag gaggcgttca tcgtccttgc
     2941 ctgctggaac gcggaatgcg tggcgtgaag ggcttccgga ccgatatccg aaggcatgtc
     3001 gaacgtgtaa acgatctttt tggaatcgga cgccggggcg gaagaggagc ttccggaagc
     3061 agagccgttt tctgcgaaag taaagggaag cccgatgggc agcttggtcg ccgatgtttc
     3121 actggagccg gttccggttc cgtcgttgat gcctgccttg cagcggggat cacgggaaaa
     3181 gccctgcag
//
LOCUS       DASTSF010000112         3588 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_597481,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000112 DASTSF010000000
VERSION     DASTSF010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3588
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_597481"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(89..2224)
                     /locus_tag="VFU15_03355"
     CDS             complement(89..2224)
                     /locus_tag="VFU15_03355"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEU4716839.1"
                     /translation="MEERKDILWRVYLVYICCCVFGLAVLGRVFYIQFAQGDYWKTQA
                     ANFMNKEEDIEAVRGNIFAKDGSLLATSLPYYEVGIDPLANSFIDDRDFEDSARLLSR
                     GMAALLGDRKADEYYRTIVGARAKGLRYQVLARNVSYKELQEMKKLALFRGGRYRGGF
                     IYVPTNHRERPFRLLAERTIGYIADSGRVRVGLEGAYDSVLTGTRGKRLMQKIAGGVW
                     MPLNNENEIEPQQGKDIVTTIDINIQDVAENALMNSLIEHNAKYGCVVLMEVATGEIR
                     AIANLTRQDSGVYTEDYNYAIADAREPGSTFKLASLLVGVDDGLVDIDQKVDLEGGHH
                     TYFDLPMRDAETPKQNVVTVKEAFWESSNVGISKVIWGAYAKTPKNFTDGLHRLRFGE
                     PLGLSIKGEGKSMIKNAGDKNWYGPSLPFVSIGYESLITPMQTLTLYNSIANNGVMVK
                     PMFVKEIREKGKTIKTFQPEIINPAVAKPQTIAKAKILLEGVVKYGTARNLSNSVYPI
                     AGKTGTAQIAKARGYGQNKNDVTYQASFVGYFPADKPKYSCIVIVNAPSGDVYYGGKV
                     AGPIFRQIADRVFATELDIHTPVNDQSNPLAEVPLIRNGIASATLAAAGGLMIDVRTG
                     ADFNPDGYVTASYSGKEADLKNISPDAQLRSGIMPDLTGMCAADVLYLMENRGYRVKL
                     KGAGSVSGQSVAPGQKISKQTVIEIELSI"
     gene            complement(2226..2639)
                     /locus_tag="VFU15_03360"
     CDS             complement(2226..2639)
                     /locus_tag="VFU15_03360"
                     /inference="COORDINATES: protein motif:HMM:NF039650.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsL-like putative cell division protein"
                     /protein_id="HEU4716840.1"
                     /translation="MTKNAIKEKPQEQEQKPKQKSSGGNIVSRTLGNVLTGAFLSKEN
                     TLRALPFIFFLTFIALCYIANGYYAEEQIRKLNKLTNDLKELRSEYIVTKSDLMFISN
                     QSEVARRAIAIGVKESVEPPKKIEVNRQPLAPATE"
     gene            complement(2665..3570)
                     /gene="rsmH"
                     /locus_tag="VFU15_03365"
     CDS             complement(2665..3570)
                     /gene="rsmH"
                     /locus_tag="VFU15_03365"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016195979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEU4716841.1"
                     /translation="MSTYHKPVLLHECLEGLNIRSEGRYVDVTFGGGGHSKAILDRLG
                     KSGRLIAFDQDEDALANAPDDPRFLLVQQNFRYLKNFLRLHQAIPVDGILADLGISSH
                     QIDEPLRGFSTRFDGPLDMRMNRNASRTAADVVNTYGEQELKKMFSEYGEVENAGRLA
                     RLIAEERGKKKITTVEDLRTIATKIAPKGKEFRYLAKVFQALRIEINAEVEALKQLLM
                     QAQEVLGPGGRLVVISYHSLEDRLVKNFFRAGNFSGEQEKDFYGNVIRPLREITRKPV
                     TPGEKEVEENSRARSAKLRIAEKPA"
BASE COUNT          705 a         1042 c          913 g          928 t
ORIGIN      
        1 gaatcgaacg tgatcccggt gatggcgaca ttcgtcgaac cttttatttc ggccaggcct
       61 gctttatata atatgtcacg caacaacttc atatagaaag ctcgatttcg ataaccgttt
      121 gttttgaaat tttttgcccg ggcgcaacgc tctggccgct taccgatccc gcacctttca
      181 gcttcacacg gtaaccgcgg ttttccatca ggtaaagcac atcggccgcg cacatgccgg
      241 tgagatcagg catgatcccg ctacgcagct gcgcatccgg actgatattt ttcagatccg
      301 cttctttacc ggaataggat gcggtgacat atccgtcggg attgaaatcc gccccggtcc
      361 tgacatcgat cataagtccg ccggctgccg caagcgttgc agaagcaatg ccgttcctga
      421 tcagcggaac ttcggcgaga ggattggact ggtcattgac cggtgtgtga atgtcgagct
      481 ccgttgcaaa gacgcggtcc gcgatctgcc ggaagatcgg tccggccact ttcccaccgt
      541 aatacacatc accgctcggc gcattgacaa tgacgatgca ggaatatttt ggtttgtctg
      601 ccggaaaata cccgacgaac gaagcctggt aagtaacatc atttttgttc tgaccataac
      661 ctctcgcttt tgcgatctgc gccgtacctg tttttccggc aatgggataa acggagttgg
      721 agagattgcg cgccgtcccg tatttcacca cgccttccag caggatcttc gcctttgcga
      781 tcgtctgcgg tttcgcgaca gcaggattaa tgatctccgg ctggaatgtt tttatcgttt
      841 tgcctttttc gcggatctct tttacgaaca tcggcttcac cattacgcca ttgttcgcga
      901 tggaattgta aagcgtcagc gtctgcatcg gcgtgatcag cgattcgtaa ccgatggaaa
      961 cgaacggaag tgaagggccg taccagttct tgtcgcccgc attcttgatc atcgatttac
     1021 cttcaccttt gatcgaaagc ccgagcggct caccgaagcg aagacggtga agtccgtccg
     1081 taaaattttt cggcgttttc gcatacgcgc cccagatcac ctttgaaatg ccgacgttgg
     1141 acgattccca gaacgcttct ttcaccgtta ctacattttg cttcggcgtt tcagcatcac
     1201 gcatgggcag atcgaaatac gtatggtgcc ctccttcgag gtccactttc tggtcgatgt
     1261 cgacgagacc gtcgtccacc cccaccagca gtgacgcgag cttaaacgta gagcccggct
     1321 cgcgcgcatc cgcgatggcg taattgtaat cttccgtgta aacgcccgaa tcctggcgcg
     1381 tgaggttcgc gatcgcgcgg atctcaccgg tggccacttc catcagcacg acgcagccgt
     1441 attttgcatt gtgctcgatg agcgaattca tcagcgcatt ttccgctaca tcctggatgt
     1501 tgatgtcgat cgtcgtgacg atatcttttc cctgctgcgg ctcgatttcg ttctcgttgt
     1561 tcagcggcat ccacacgccg ccggcgatct tctgcatcaa tcgttttcct cttgtaccgg
     1621 tgagcacgga atcatacgcg ccttcaagtc ccacgcgcac acgcccgctg tcggcgatgt
     1681 aaccgatggt gcgctccgcc agcaggcgga acgggcgctc gcgatgattc gtaggaacgt
     1741 agatgaaacc gccgcggtat ctcccgccac ggaagagcgc gagctttttc atttcctgca
     1801 gctccttata agaaacgtta cgcgccagca cctggtaacg caaacccttt gcgcgcgcac
     1861 cgacgatggt gcggtaatat tcatccgctt tgcggtcgcc gagcagcgcc gccatgccgc
     1921 gcgaaaggag acgcgcgctg tcttcgaaat ccctgtcgtc aatgaagctg tttgcgagcg
     1981 gatcgatgcc gacttcataa taaggaagag aagtggcgag aagagatccg tccttggcga
     2041 aaatatttcc acgcacggct tcgatgtctt cttccttgtt catgaaattc gccgcctgcg
     2101 ttttccagta atcgccctgc gcgaactgga tatagaaaac gcggccgaga acggcgagac
     2161 cgaacacgca gcagcagatg tacacgaggt acacgcgcca gagtatgtct ttgcgttctt
     2221 ccattttatt cggtggccgg tgcgagcggc tgacggttta cttcgatttt tttcgggggt
     2281 tcgacagatt ccttcacacc gatcgcgatt gcccgcctgg caacttccga ctggttgctg
     2341 atgaacatga gatcggattt ggtgacgatg tattccgaac gcagctcctt caggtcgttc
     2401 gtcagcttgt tgagcttgcg tatctgttct tccgcataat acccgttcgc gatgtagcag
     2461 agcgcgatga acgtgaggaa gaaaatgaat gggagggcgc gtaacgtatt ctctttcgag
     2521 aggaaggccc cggtgagcac attccccagc gtacgggaga cgatgttccc tccgctgctt
     2581 ttctgcttcg gcttctgttc ctgttcctgt ggtttttcct taattgcgtt ttttgtcata
     2641 gtccgcgttt gcggcttttc cgcctcaggc gggtttttct gctattctca gtttcgcgct
     2701 cctcgcgcga ctattttctt cgacctcttt ttccccgggc gtcacgggtt tccttgttat
     2761 ctcgcgcagc gggcggatca cgttcccgta gaaatccttc tcctgctccc cactgaaatt
     2821 tcccgcccgg aaaaaattct tcacgagcct gtcttcgagc gagtggtagg aaatcaccac
     2881 cagcctgcct cccggcccga gcacttcctg cgcctgcatc agcagctgct tcagcgcttc
     2941 cacctctgca ttgatctcta tccgcagcgc ctggaaaacc tttgccaggt accggaactc
     3001 ttttcctttc ggcgcgatct tcgtcgcgat cgttctcagg tcttccacgg tcgtgatctt
     3061 tttctttccc cgctcttcgg caatgagccg ggcaagcctt ccggcattct ccacctcgcc
     3121 gtattcactg aacattttct tcagctcctg ctcaccgtac gtgttcacca catctgccgc
     3181 cgtgcgtgaa gcgttcctgt tcatgcgcat atccagcgga ccgtcgaagc gcgtggaaaa
     3241 tcccctcagg ggttcatcga tctggtgcga agagatcccg aggtcggcga gaattccatc
     3301 caccggtatc gcctggtgaa ggcggagaaa atttttcagg taacggaaat tctgttgcac
     3361 cagcaggaac cgcggatcgt caggcgcatt cgcaagggcg tcttcgtcct ggtcgaatgc
     3421 gatcagcctt ccgcttttcc cgaggcgatc gaggatcgct ttcgagtgac cgcctccacc
     3481 gaatgtcaca tccacgtacc tgccttcgga ccggatattc aatccttcga ggcactcatg
     3541 taaaagaacg ggcttatggt aggtcgacat cctccgcttt gttaccca
//
LOCUS       DASTSF010000113         4624 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1267154,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000113 DASTSF010000000
VERSION     DASTSF010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4624
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1267154"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..722)
                     /locus_tag="VFU15_03370"
     CDS             complement(<1..722)
                     /locus_tag="VFU15_03370"
                     /inference="COORDINATES: protein motif:HMM:NF025600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHYH protein"
                     /protein_id="HEU4716842.1"
                     /translation="MKKAFFFGAMLISAASFAQLTPEITSWVINTTGATGYNSIPSDV
                     QTVQYSATQVYVSCTCIPGYSIGPWNANPNTPTNQNFVYKITRNPVQNTGTPVTTPLG
                     HIGVWSNGVSVFNAKDGMTYNNAGVWNRNAYYYEGVSFDNCLGHPAPNGEYHNHVNPR
                     CLYSDTDSLNHSPIIGYAFDGYPIYGAYAYTNTNGTGPIKRMQSSYVLSTATTRANGP
                     NVDATYPAGCFCEDYVYQAGSG"
     gene            complement(819..1121)
                     /locus_tag="VFU15_03375"
     CDS             complement(819..1121)
                     /locus_tag="VFU15_03375"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU4716843.1"
                     /translation="MAVSKDPTKKFLAAVGEKIRHYRLKRGLTLEALGEDIGLDKANV
                     HKIESGQNITLRTLLKVAAFLEVKPVDLISDGPLMAYEEAEDYVRRQKRKRKASRR"
     gene            complement(1359..1775)
                     /gene="msrB"
                     /locus_tag="VFU15_03380"
     CDS             complement(1359..1775)
                     /gene="msrB"
                     /locus_tag="VFU15_03380"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006727211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEU4716844.1"
                     /translation="MNTDTTKITKTDDEWKKTLTPTQYNILREKGTERAFTGAYWDNH
                     DKGIYKCAACGSVLFSSDEKFDAGCGWPSFSLPWDSSAVEYHIDTTFGMVRTEVLCRK
                     CGGHLGHVFDDGPPPTGKRYCINSGAMIFVPDEKKQ"
     gene            complement(2032..3339)
                     /locus_tag="VFU15_03385"
     CDS             complement(2032..3339)
                     /locus_tag="VFU15_03385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716845.1"
                     /translation="MKITRENCEAFFLDFYEGRLSTEQVTELFAFLDDNPEQKEMFES
                     FEDVSFNPDEKVSLGDKSFLKKDAAAGDAVTLSGYEHYFIAYVEGLLSAEEKQAVEKF
                     IAAHPQKRAELELLKKTVLAPEKEIVFENKEALKRSVVLTEKNFEEYAIASVEGLLNA
                     GEQEALEKFAAGSEKYARELSLLAKTKLQPDLSVVYENRNELKRRRGGFYWMRDVRFA
                     AAAAVFLFVFIAWWNTGDKGAQVKSGNAIAGNVNAGKTGDPAAHGNSSVSRPGDNANT
                     PVQLAMEQDPHVKNDRTDKVPAVQNEAHYQFASLAPREVRSLKRGDVQDDVDFSDAFY
                     LAADYSGSGSAAASTPSFGQFAKRWVKNKLDGGRTDDQDGDMLYAMNGAKTSGAKEVT
                     NFDLTSSAINRIGRSTGTNLHLGRQSNKTILTFGKRDFVIAKN"
     gene            complement(3347..3832)
                     /locus_tag="VFU15_03390"
     CDS             complement(3347..3832)
                     /locus_tag="VFU15_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010662262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEU4716846.1"
                     /translation="MTAEEYNRCVDLYADNLYRFILKNVKDKDKAKDIVQDTYEKLWL
                     KVSETEAKNAKSYMFTTAYRTMIDYLRKDKRQGTMEEAPMIAVSHNRQYSDLKEILNE
                     ALNRLPEIQKSVVLLRDYEGYSYEEIGEITGLNESQVKVYIFRARAFLKNYIGSIEKV
                     V"
     gene            complement(4333..>4624)
                     /locus_tag="VFU15_03395"
     CDS             complement(4333..>4624)
                     /locus_tag="VFU15_03395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716847.1"
                     /translation="AKFKTMLKSDILSMEEPGEIASLINPWVYKDYEKRRLVLGELDA
                     MDLATVNKAVKKYFTPDSYKLIIAGDPVALAGQLGKIQGLRKIELSEIERDQ"
BASE COUNT          994 a         1263 c         1194 g         1173 t
ORIGIN      
        1 tcgcccgagc cggcctggta aacgtaatct tcacagaaac atcccgcggg atacgtggcg
       61 tccacgttgg gtccgttcgc acgcgttgtt gcggtggaaa gcacgtagct gctctgcatg
      121 cgtttgatcg gaccggttcc gttcgtattc gtataagcgt atgcgccgta gatcgggtag
      181 ccgtcgaacg cataaccgat gatcggtgaa tggttgaggc tgtccgtgtc gctgtacagg
      241 cagcgcgggt tgacgtggtt atggtattct ccgttcggag ccgggtgccc gaggcagtta
      301 tcgaagctga cgccttcata ataataggcg ttacggttcc acacgccggc attgttgtag
      361 gtcatgccgt ccttcgcatt gaaaacggaa accccgttcg accacacgcc gatatgcccg
      421 agcggagtgg tgacgggcgt gcctgtattc tgcaccgggt tacgcgtgat cttgtaaacg
      481 aaattctggt tggtgggcgt gttcgggttg gcgttccacg gaccgatgct gtagccggga
      541 atgcacgtac agctgacgta tacctgcgtt gcggagtact gcacggtctg cacgtcggac
      601 gggatactgt tgtatcccgt agcgccggtg gtgttgatga cccaggaagt gatctccggt
      661 gtgagttgcg caaacgaagc tgcggaaatg agcattgcgc cgaagaagaa agcctttttc
      721 ataagtgagc gtttagcgtt gtagagggtt agacgccgct tttccgcgga actttcgcgg
      781 gacagtattt tttttgcgga aatgcggtga atgccgggtt accggcggga agccttgcgt
      841 tttcttttct ggcggcgcac ataatcttcc gcctcctcgt acgccatcag cggaccgtcg
      901 gaaataagat ccaccggctt cacttccagg aacgccgcca ctttgaggag cgtgcgcagc
      961 gtgatgttct gccctgactc gatcttgtga acgttggctt tgtcgaggcc gatatcctcc
     1021 cccagcgctt cgagcgtcaa tcctcttttc agccggtagt gacggatctt ctccccgaca
     1081 gctgccagga attttttcgt agggtctttg gatactgcca taaccctaca aaaatttaga
     1141 gatttatttt aaaaaagttg tgttttaata taacatttcg taagcttgcc gtaacgtccc
     1201 ggttgataaa acccgccggg aacgaaataa aaaaagcctc tctttcaaaa ggcttttcac
     1261 agtaaacgcg ttataaagct acaggaccca cctgtatcat tcacatgcaa ggccgcatgt
     1321 gacgggaagc cggacccgcg caagcagtcc ggcttttttc attgcttctt ttcatcgggc
     1381 acgaagatca tcgcgccgga attgatacaa taacgtttcc cggtgggcgg cggaccgtca
     1441 tcgaacacgt ggccgagatg accgccgcat ttccggcaga gcacttccgt acgcaccatt
     1501 ccgaatgtcg tgtcgatgtg gtattccacc gcgctcgaat cccagggaag cgaaaagctc
     1561 ggccagccgc agcctgcatc aaacttctca tcggaggaaa acagcaccga gccgcaggcc
     1621 gcgcatttgt aaatgccttt gtcgtgattg tcccagtagg cgccggtgaa ggcgcgctcg
     1681 gttccttttt cgcgcaggat gttgtattgc gtgggcgtga gtgtcttttt ccactcatcg
     1741 tcggtcttcg tgatcttcgt cgtgtccgtg ttcatggcgt tctgttttaa cgtgtccgca
     1801 gctacgttcg tttttcccgg tatcgtgtcc ggactggcac agctgcctaa aagtaccagc
     1861 aaggtgagcg tctgaataaa catcttcatc ggttttttca ggatatacgg caattcaccg
     1921 ctgcccggat tcgtaagcaa agatcgggga gattaataaa tgttaaaagg ggaaacgcag
     1981 gaggtaagct acgcggtgga aatatcacag gagaaaaaac agggaaccgt tttaattctt
     2041 cgcgatcacg aaatcgcgct tgccgaacgt gaggatggtt ttgttgctct gccggccgag
     2101 atgcaggttc gttcccgtgc tgcgcccgat gcggttgatc gcggaagatg tgagatcaaa
     2161 gttcgtcact tctttggcac ccgaagtttt cgcgccgttc atcgcgtaca gcatgtcgcc
     2221 gtcctggtcg tccgtccttc ccccgtcgag cttgttcttt acccagcgtt tggcaaactg
     2281 cccgaaagaa ggtgtggaag cggcagcgga acccgagccg gaataatccg ccgcgaggta
     2341 gaacgcatct gaaaaatcaa catcgtcctg cacatccccg cgtttgagcg aacggacttc
     2401 gcgcggcgcg agggaggcaa actggtaatg tgcttcgttc tgcaccgcag gaaccttgtc
     2461 cgtgcggtca tttttcacgt gaggatcctg ctccatggca agctgaaccg gcgtgttcgc
     2521 attatccccc ggacgggaaa cggaagaatt gccatgcgcc gcagggtcac cggttttgcc
     2581 tgcgttcaca tttcctgcga tggcgttccc gcttttcacc tgtgcgcctt tgtcgccggt
     2641 attccaccac gcgatgaaca cgaaaaggaa aacagcagcg gcggcggcaa accgcacgtc
     2701 gcgcatccaa tagaatcctc cgcggcggcg cttcagctcg ttcctgtttt cataaacaac
     2761 ggacaggtcg ggctgcagct ttgtttttgc aagaagcgaa agctccctcg catatttttc
     2821 actcccggcg gcgaactttt ccagcgcttc ctgttcgcct gcgttcagca aaccttccac
     2881 ggaagcgatc gcgtactctt cgaaattctt ctccgtcagc acgacggatc gcttcagcgc
     2941 ctctttattt tcaaaaacaa tttctttctc cggcgcaaga acggttttct tcagcagctc
     3001 cagctccgcg cgtttctgcg ggtgcgcggc aatgaatttc tccaccgcct gcttctcttc
     3061 cgccgaaagc aatccttcca cgtacgcaat gaaataatgc tcgtacccgg aaagcgtaac
     3121 ggcatcgccc gcggcggcat ccttcttcag gaagctcttg tcgccgagcg aaactttttc
     3181 atccgggttg aacgaaacat cctcgaagct ttcaaacatt tccttctgct ccgggttgtc
     3241 atcgaggaac gcgaacagct ccgtcacctg ctctgtcgac aggcgcccct cgtaaaaatc
     3301 gaggaaaaag gcttcacagt tttcgcgtgt aattttcatg ggtcaatcaa accactttct
     3361 ctatgcttcc gatgtaattt ttcaggaacg cccgcgcacg gaaaatgtaa actttcacct
     3421 gcgattcgtt cagtcccgtt atttcaccga tttcttcata cgaatatcct tcatagtcac
     3481 gcagcagcac caccgatttc tgtatttccg gcaaacggtt cagcgcttca ttcagtattt
     3541 ccttcaggtc gctgtactgc cggttgtgcg acacggcgat catcggcgct tcttccatcg
     3601 tcccctggcg cttatccttg cgcaggtaat cgatcatcgt ccggtaggcc gtggtgaaca
     3661 tgtacgactt tgcgttcttc gcttccgttt ccgaaacctt cagccacaat ttttcatacg
     3721 tgtcctgcac gatatctttc gccttgtcct tatccttcac atttttcagg ataaaacgat
     3781 aaaggttatc agcgtacaga tcgacgcaac ggttatattc ttctgcggtc atgcggtcag
     3841 gtgtgcattc cgtacctaag acgggctgct ttgcccggtt gttacagcaa aagtaaagta
     3901 tttcacaagc tactggttct gaatgatttg tagcgaaccg cctgcgtttc aaaccaccgt
     3961 ccgctccggc tatccgcgct ttgcgctcct atccgggctg gtcattcgtt cctccttcac
     4021 cgcaaatgtt ctgctgtatc cgctccccgg tcagaattcc tggttgaaag gtggaaagtc
     4081 agaacggcag gcgagaagct gaaaaccgtt ttttaaccac gaagaacaca aagttttttc
     4141 acaaagtgca cggaggaaaa cgaaggtaat tctgtttcaa agttggaaag ttgaaaggtt
     4201 ggaagtcaaa accacgaagt acacgaagct tgtcacaaag tgcgcggagg aaagcaggaa
     4261 acggtcaacg ctgtccagtc ccggacattg gaaaaactga aaatccaaaa ctcgaaactt
     4321 gaaacccgga acctattggt cccgctcaat ctcgctcagc tctatcttcc gaagcccctg
     4381 tatttttccc agctgcccgg ccagcgcaac cggatccccg gcgatgatga gcttgtagct
     4441 gtccggcgtg aaatattttt tgacggcctt gttcacggtg gccaggtcca tggcatcgag
     4501 ctcgccgagg acgagcctgc gcttttcgta atctttatac acccacgggt tgatcagcga
     4561 cgcgatctcg ccgggctcct ccatgctgag aatatccgat ttcagcatcg tcttgaattt
     4621 cgcc
//
LOCUS       DASTSF010000114        13160 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_150550,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000114 DASTSF010000000
VERSION     DASTSF010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 13160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13160
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150550"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..520)
                     /locus_tag="VFU15_03400"
     CDS             complement(<1..520)
                     /locus_tag="VFU15_03400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716848.1"
                     /translation="MIPRSLDKQKIAPVRYESDTLRQVAVEVQRPDSIHGIPGKARLP
                     HKSQYVVPLPFVGFFGANVLMSPGQPTLDPLADNLNSALKHEISLNYPQTLNNKYTLK
                     VKVDSSDFDIRYRKKGYLVVLFFVWFGSKKEYCTMDKLSLSASYELYDGDSLVKKGAV
                     TKNNSSNEKILGS"
     gene            complement(717..1178)
                     /locus_tag="VFU15_03405"
     CDS             complement(717..1178)
                     /locus_tag="VFU15_03405"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019825474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU4716849.1"
                     /translation="MKKNDITWQCKPFSDLRAAELHRLLSLRCAVFVVEQNCPYLDPD
                     HKDEHALHVMGWKGSELVACARILPAGISYDEVSVGRVATSSSVRGKGVGRELMKKTM
                     EYIRTVFGDVPVRISAQSYLQIFYEDLGFKRTKKKEYLEDDIPHVEMLLGV"
     gene            complement(1175..1573)
                     /locus_tag="VFU15_03410"
     CDS             complement(1175..1573)
                     /locus_tag="VFU15_03410"
                     /inference="COORDINATES: protein motif:HMM:NF016763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEU4716850.1"
                     /translation="MKKNVFLPAILWALLILILCGLPGKDIPHISFLELLSFDKLVHA
                     GIFFVLTVFGIKGCRLQKRSAYMKEHAAFLSAGISSAYGGLMEILQGTIFIGRTADIF
                     DFIADSIGCVAAVWLYPYISRKIPRLNLPL"
     gene            complement(1585..1965)
                     /gene="gcvH"
                     /locus_tag="VFU15_03415"
     CDS             complement(1585..1965)
                     /gene="gcvH"
                     /locus_tag="VFU15_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HEU4716851.1"
                     /translation="MNFPGELKYTKDHEWVRVDGGIATIGITDFAQGELGDVVYVEIE
                     TVGEAISREDVFGTVEAVKTVSDLFMPVSGEVLEKNEGIDANPEVVNKDPYGEGWMIK
                     VKITDPSELDKLMDAAAYKALIGQ"
     gene            complement(2058..9392)
                     /gene="sprA"
                     /locus_tag="VFU15_03420"
     CDS             complement(2058..9392)
                     /gene="sprA"
                     /locus_tag="VFU15_03420"
                     /inference="COORDINATES: protein motif:HMM:TIGR04189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HEU4716852.1"
                     /translation="MNLFAKFGIAVVASVVLLTAVMNSDARIASPYRGLVGGTDSPAG
                     GDTLPYPFQDQSYLDPMYYTNDAPLKLNDPSNIKTTVTYNPQRGDSSTYDVQQRIGNR
                     LDYRPPTYMTFDEYEYYDLKKSVHDYWRQREHAETMNQRGQNKNLIPPIKLGKLGQDI
                     FGGGSIDIKPQGSAELIFGININKTDNPALPVRQRRISTFDFDEKIQLNVIGKIGDKL
                     KLTTNYNTESTFDWENQMKLDFTGYEDDIIKKIEAGNVSFGLNTSLITGSQTLFGIKT
                     QFQFGRLTMTNIISQERGKKSEIDVTGGAQVSTFEVTADNYEANKHFFLSQYFYNHYD
                     QALANFPVINSGVQIVRTEVWVTNRTGAVDNTRNIVAFSDIGEGTGDLENNLVPGGIT
                     INTTEDPVPHNKLNTLYDDLTNTYSSVRDISVPLTTSLAPLITAYNFTSSVNFERVNN
                     ARKLANTEYSVNSRLGYISLNQALNYDEVVAVAYQYTLNGKTYQVGEFSDGGVTGQNA
                     ILVKMLKSTNNVPRVTSPTNVNGVRFKTWDLMMKNVYSIGAYQVNPQNFQLQVWYNNP
                     STGTDIPFIPEGPINGKPLLQVLNLDKLNQQQQASPDGQFDFIDGVTINSSNGRVIFP
                     VVQPFGGWLAGKLSDPGLITKYCYQTLYDSTKTFAQTVDTQHDRFKLKGSYQSSSNSD
                     ISLNAVNIPQGSVVVTAGGAPLQENVDYTVDYTLGRVKIINESILNSGVPIKISLESN
                     SLFNIQSKTLWGTHLDYKFNKDFSIGGTVMNLTERPITQKIQVGDEPMSNTIMGMDFN
                     YRTDAPILTRMVDKIPLIDTKAPSSITAQGEFAYLHPGHNKVIGKTGQSYIDDFEGTQ
                     SAIDIRSPQGWSLASVPQGQPQRFPEATQDSVMSGYNRAKLCWYVIDPLFLRPDNNLK
                     PGYITTADQSDHRVREVLETEVFPNKQPPNGQPLNVSVLDLAYYPNDRGPYNFDCNPT
                     SFSSGLDSSGHLLNPASRWGGIMRRIETNDFEAANIEFIQFWMMDPYNDDVPQNQRNT
                     TGELVFDLGNISEDVLKDSRNSFENGLPTSDTATSLVNYTPWGRVPIVQSIVNAFDND
                     PDKRKMQDVGLDGLGDDNETTYFASYLNQVSARWGTNSVAYQNAVKDPAGDNYHYYRG
                     TDYDNAQVSILDRYKMFNGVDGNSPATGQPNDDGYTESYPTQATTLPNQEDINRDNTL
                     NENESYYEYRVKLTPNDINPNNNGNNYITDVFQTTVTTKDGRVRPITWYQFRIPIQSY
                     TTKVGNIENFNSIRFIRIYFKDVDKPVICRFARLELVRSDWRKYNYSLLSPGEYISND
                     QSNTEFDLAAVNIEENGNKTPVNYVLPPGIDREQNVQSATLVRLNEQALSMKVCDLED
                     GDSRACYKTVNNLDVRSYKKIRMFIHAEARAGSTTPLNNGDLVGFIRMGSDQTDNYYE
                     YDIPLQVTPAGNYSPNDETDREKVWPVANNLELPFELLQYVKQQRNIAIANGTASLTQ
                     EFIMEDPDNPGRFVRVKGNPNLSMLKVFMLGVMNPKKTNSTPGDDGLSKCAEIWFNEL
                     RLTDFDEHGGYAATARVTAKLADFALINLSGNMATPGFGSIEKKVSERLRENQLNYDV
                     SAQVELGRFFPQKSGIRIPLFWGYGETFITPQYNPLDPDILLKPILSNEDLSRDLRDS
                     IRGATVDYTRRRSLNFTNVHKERLKGATKVYPWDISNFTFSYAYTSVYRHNVNLEYSL
                     LANQRGGFTYTYNNPVKPIKPFDKSPWAKSKWMRLVREFNFTPAPTMFGFSTDLNRSY
                     SEIKNRNITGFSDVFVPVNYNKSFLWTRNYDMRWDICKNLKLDFSADNIGTVLEPYGK
                     IDTPSEKDSLLRSIRGFGTTTSYHHTTNVTYNVPINKIPVFDWINPTVRYSSGFIYTR
                     APFSADTVGNVIGNNAKWDYTVQLTMSTLYGKSKYLKKVMNKRPGQDNQSQTPSKQSN
                     KGPGGPSANPQDTTKKKDNQYEVLEYLARLMMSVRNVSLSYSVNSTMGLPGYDRWTQF
                     LGFDNHFDGPGVGFLFGQQTGFGPDNLDYPMWAAEKGWLVHTPSLFTPFTQGTSTNLT
                     GRAGLEPFPDLKIDLTINRTYSDNKSEYFRWDDLSNSYVVQSPTETGSFSISIITWHT
                     AFTRDRKEDHFSKVFQQFLNNRAVISGRLGGQNPYSTGQSVTGFYDGYSNVQQDVIIP
                     AFVAAYSGKRAETTSLGLFPAIPMPNWRLTYDGLSKYKKVKKYFKTVTLSHSYRSTYS
                     LGGFQTNLDYVDADGDGFSDNVRNVSGDFQSKYQISTVQISEQFAPLVGVDLSWNNSL
                     TTHIEYKKDRTLSLSLANTQLTEISGKEIVLGAGYRIPKLPIPFLKKLMKGKTPMSDL
                     DIRCDVSYRNNQTVIRKAVEGLNVLTAGQDIFSLKTSVSYQLTQNLQIRLFCDRIMTN
                     PLISSSFKTANTNAGISLRFML"
     gene            complement(9411..9998)
                     /gene="ruvA"
                     /locus_tag="VFU15_03425"
     CDS             complement(9411..9998)
                     /gene="ruvA"
                     /locus_tag="VFU15_03425"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008467374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEU4716853.1"
                     /translation="MFNHFEGKITMKTPTYTVVDCGGVGYLMNISLHTYSRIPDSGNF
                     RLFAHLAVREDAMVLYGFADEEEREIFRQLISVSGVGPGTARMILSSMSPSEIVQAIH
                     AGNVSALRSVKGIGEKSAQRIIIDLKGKMGKGTEGISAVSFGGDNKMRDEALTALVTL
                     GFARNVAEKAIDKALASEGKAVSVEHLLKIALKNL"
     gene            complement(10004..12277)
                     /locus_tag="VFU15_03430"
     CDS             complement(10004..12277)
                     /locus_tag="VFU15_03430"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017843161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HEU4716854.1"
                     /translation="MAKFRKQDALDYHSQGRPGKIEVLPTKPYSSQFDLTLAYSPGVA
                     EPCLEIEKNPEDAYRYTSKGNLVAVISNGTAVLGLGNIGSLASKPVMEGKGMLFKIYA
                     DIDVFDIEVDTTDIDSFVSTVKAISPTFGGINLEDIKAPECFEIERRLKEELSIPVMH
                     DDQHGTAIISSAAMINACELAGKNIGEVKIVVNGAGASAISCAKMYIAVGAKKENITM
                     LDSKGVLTTDRTDLDEYKKFFAKKSAGVSTLAEAMKGADVFVGLSKGNVVTPDMVRSM
                     AAKPVVFALANPDPEISYEDATGARPDIIMATGRSDNPNQVNNVIGFPFIFRGALDVR
                     ATQINEAMKLAAAKAIAALAKEPVPDDVNHAYNTRNLSFGENYIIPKPIDSRLISTVS
                     LAVAKAAIESGVARRMITDWDAYAIELSNRLGRDNKLTRGLATKAKQNPKRIVFAEAD
                     TYKILKAAQVVRDEGIARPILLGDVAKINRLIEENNLDLGDTPIIDPRDPSQETKRTE
                     FGEIFFQRRKRRGYTLYESKKAMQDRNYFGAMMVETGMADGMISGLTRKYATPILPAL
                     HCIGVQEGVNRVAGMYILSTRAGNFFFADTTMNVDPTAEELVDITVLTAQAIRQYNIV
                     PRIALLSYTNFGSAPGEVPEKVRKAVEILHRDYPGLIVDGDVQANFALNEELLKEQFP
                     FSELVGKKVNTLIFPNLASGNIAYKLIQVMGDSEAIGPILLGLKKPVHVLQLGSSVRE
                     IVNMVIIAVVDAQSRRS"
     gene            12493..>13160
                     /locus_tag="VFU15_03435"
     CDS             12493..>13160
                     /locus_tag="VFU15_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017843160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HEU4716855.1"
                     /translation="MSAYRRIVVFTGAGVSAESGISTFRDSGGLWEKYDIRDVATPEA
                     WMKNPNLVLEFYNQRRRQVMDAKPNPAHVSLAELEKKFDVHIITQNVDDLHERAGSKN
                     VLHLHGEIRKARSTKNPELIYPVKGWEIKAGDKCELGSQLRPHIVWFGEFVPEMDRAQ
                     EIAAGAELFIIIGTSLNVYPAAGIIHFVKRDVPIIIIDPNEINNPGIKGITFIRSNAG
                     AGVPV"
BASE COUNT         2889 a         3503 c         3414 g         3354 t
ORIGIN      
        1 tcgagcccag tatcttttca ttgctgctgt tgtttttcgt aacggcccct ttcttcacca
       61 gtgaatcgcc atcgtacaat tcatagctcg cgctaagcga aagtttatcc attgtacaat
      121 attccttttt tgatccgaac caaacgaaaa agagcaccac taaatatcct tttttccggt
      181 aacgtatgtc gaaatcacta ctgtcgactt tcaccttaag tgtgtacttg ttgttcagcg
      241 tttgcggata gttcagcgat atttcatgtt taagcgctga attcaggtta tcggcgagcg
      301 gatcaagcgt gggttgcccg ggagacataa gcacgttggc accgaaaaaa cctacaaatg
      361 gcaggggaac cacgtactgt gatttgtgag gcaggcgcgc tttgccggga attccatgaa
      421 tgctgtcggg tctttgcact tcaacggcca cctgccggag cgtgtctgat tcatacctga
      481 ctggcgcaat cttttgtttg tcgagactgc gcggaatcaa gccgtaaaat gtgcaacctc
      541 caaggagaat ccctgcgagt agaagaagaa atccgttttt tttttcattt gatcggggtt
      601 ttaattaacc cgcgaaagtt atctgttttg ggttcccaaa caaagacacc gcacgagaac
      661 caatcgtgaa aagtaattgc tcctgaaaaa ccttttcaac ctctgacttt caactttcaa
      721 accccgagca gcatctccac gtgcggaatg tcatcctcca ggtattcttt cttctttgtc
      781 cgtttaaacc cgaggtcttc ataaaatatc tgcaggtacg attgagccga aatgcgcacc
      841 ggcacatcac cgaaaaccgt gcggatgtat tccatcgttt ttttcatcag ctccctgccg
      901 acgcctttgc cgcgcacgga agaagacgtg gccacccttc ccaccgaaac ttcgtcatac
      961 gagatcccgg ccggaagaat ccgcgcgcag gccacaagct cactcccttt ccatcccatc
     1021 acgtgcagcg cgtgctcgtc cttgtgatcg ggatcgaggt acgggcaatt ctgctccacc
     1081 acgaacacgg cgcagcgcaa tgaaagcaaa cggtgcagct ctgcggcgcg caggtcgctg
     1141 aacggtttgc attgccacgt gatgtcgttc tttttcacaa gggtaaattc agccggggaa
     1201 ttttgcgcga aatataagga tagagccaaa cagccgcaac gcatccgatg ctgtctgcga
     1261 tgaagtcgaa aatatcggcc gttcttccaa tgaagatcgt tccctgcagt atttccatca
     1321 gcccgccgta cgccgaagaa attcccgctg aaagaaacgc cgcatgctcc ttcatgtaag
     1381 cgctgcgttt ctgcaggcgg cagcccttta tcccaaacac ggtaagcacg aaaaaaattc
     1441 ctgcgtgaac gagcttgtca aagctcagca gctcgagaaa agaaatgtgc ggaatatcct
     1501 taccgggtaa tccgcacaga atgagaataa gcagcgccca gagaatggcg ggaagaaaaa
     1561 cattcttctt caccggcgcc ggctttattg accgataagc gctttgtaag ccgcggcgtc
     1621 catcagctta tccagctcgg acggatccgt tattttgact ttgatcatcc agccttcccc
     1681 gtaaggatct ttgttcacca cttcgggatt ggcatcgatg ccttcgttct tttcgaggac
     1741 ttctccggaa accggcatga aaagatcgga aaccgttttc actgcttcca ccgttccgaa
     1801 aacatcttcc cgtgaaatgg cttcacccac ggtttctatt tctacataaa caacgtcgcc
     1861 aagttcgccc tgcgcgaagt ccgtaattcc gatcgtagcg atgccgccat ccacacggac
     1921 ccattcgtgg tccttcgtgt atttcaattc tccgggaaaa ttcatagatg tgtgttgttc
     1981 tgaaatgtga ggaacaaagt taattttttt tggaaacggc ccgggatgat gtgcgtcagg
     2041 aagaatgcat tccgccttta cagcatgaac cggaggctga tacccgcatt cgtgttcgcc
     2101 gtcttgaacg aagaagagat cagcgggttc gtcatgatgc ggtcacagaa caggcggatc
     2161 tgcaggttct gcgtaagctg gtagctcacc gacgttttca gggagaaaat atcctgcccg
     2221 gctgtcaaca cgttcagtcc ttccaccgct ttgcggatca ccgtctggtt gttccggtag
     2281 gaaacgtcgc agcggatgtc aagatcgctc atcggcgttt ttcctttcat cagcttcttg
     2341 aggaacggga tcggcagctt gggaatgcgg tacccggcgc ccaggacgat ttccttgccg
     2401 gaaatctcgg tgagctgcgt gttggcaaga ctgaggctca gcgtacggtc ttttttgtat
     2461 tcgatgtgcg tcgtgagact gttgttccag ctcaggtcga cacccaccag cggggcaaac
     2521 tgttcggata tctgcacggt gctgatctgg tatttgctct ggaaatcgcc gctcacgttg
     2581 cgcacgttat cggagaatcc gtcgccgtcg gcatccacat aatccaggtt cgtctggaat
     2641 ccgcccagtg aatacgtgga tcggtaagaa tgtgaaagcg tcaccgtttt gaaatacttt
     2701 ttgaccttct tgtatttgga aagaccatca tacgtcagtc gccagttggg catcggtatg
     2761 gcggggaaca ggccgagcga agtcgtctct gcgcgcttgc ccgaataagc tgcgacaaac
     2821 gcgggaatga tgacgtcctg ctgcacattg ctgtacccgt cgtagaatcc ggtcaccgat
     2881 tgccctgtgg aatacgggtt ctgtccgccg aggcggcccg agatcaccgc gcggttgttc
     2941 aggaactgct ggaatacttt cgagaaatga tcctccttgc ggtcgcgtgt gaacgccgtg
     3001 tgccaggtga taatggaaat gctgaagctt cctgtttctg tcggcgactg aacgacgtac
     3061 gagttggaaa gatcatccca gcggaaatat tcgctcttgt tatccgaata tgttctgttg
     3121 atggtgagat cgatcttcag atcagggaac ggttcgaggc ccgcacgccc ggtaaggttc
     3181 gtcgaggtgc cctgcgtgaa tggcgtgaag agcgaaggtg tatgcaccag ccatcctttc
     3241 tccgccgccc acatcgggta atcgaggttg tcaggaccga aacccgtctg ctgcccgaaa
     3301 aggaacccga ccccgggacc gtcgaagtga ttgtcgaaac caaggaactg cgtccaccgg
     3361 tcgtatccag gcaatcccat cgtggaattc accgaatagc ttaaggaaac gttccgcacc
     3421 gacatcatca gccgagccag gtattcaagc acttcgtact ggttgtcttt cttctttgtc
     3481 gtatcctgcg gatttgcgct gggtccgccg ggacctttat tcgactgttt cgacggcgtc
     3541 tggctttgat tatcctggcc gggccgcttg ttcattacct tcttgaggta tttcgatttg
     3601 ccgtacagtg tggacatcgt aagctgaacc gtgtagtccc atttcgcatt gttgccgatc
     3661 acgttaccga ccgtatccgc gctgaacgga gcacgtgtgt atataaaccc ggaagaataa
     3721 cgcaccgtcg ggttgatcca gtcgaacaca ggtatcttat tgatcggaac gttatacgtc
     3781 acgttagtgg tgtggtgata gcttgttgtc gttccgaaac cgcggatgct ccgcagcagg
     3841 ctgtctttct cgctcggcgt gtcgatcttt ccgtaaggct cgagcacggt tccgatgtta
     3901 tctgcgctga aatcaagctt caggtttttg cagatatccc agcgcatgtc gtaattccgc
     3961 gtccacagga atgatttgtt gtagttcacc gggacgaaca catccgagaa ccccgtgatg
     4021 ttccggttct ttatctcgct gtacgaccgg ttgaggtctg tcgagaaacc gaacatcgtc
     4081 ggcgcaggtg tgaaattaaa ttcccgcacg agcctcatcc atttcgattt cgcccatggc
     4141 gacttgtcga acggcttgat cggtttcacc gggttattat aggtgtaggt gaaaccaccc
     4201 cgctggttgg ccagcaggct gtactcgagg ttgacgttgt gccggtacac gctcgtgtac
     4261 gcgtaactga acgtgaagtt ggaaatatcc catggatata ctttcgtcgc gcctttcagc
     4321 cgctccttgt gcacattggt gaagttcaga cttcgtcgcc gggtatagtc aaccgtcgcg
     4381 ccccggatcg agtcgcgaag atccctcgac aggtcctcat tgctcaggat cggtttcagg
     4441 aggatatccg ggtcaagcgg gttgtactgc ggcgtaatga atgtttcacc gtaaccccag
     4501 aaaagcggga tgcggatgcc actcttctgc gggaaaaacc gtccgagttc cacctgcgcc
     4561 gaaacgtcgt agttcagctg gttctcacgc aggcgttcgc tcactttctt ctcgatgctt
     4621 ccgaatcccg gcgtcgccat gttcccgcta aggttgatga gcgcgaaatc cgcgagcttc
     4681 gccgtcacac gtgctgttgc ggcgtatcct ccgtgctcgt caaaatccgt caggcgcaat
     4741 tcattgaacc agatctcggc acatttgctg agcccgtcat ctcccggcgt tgaattcgtt
     4801 ttcttcggat tcatcacacc cagcatgaat actttcagca tggacaggtt cggatttcct
     4861 ttcacccgca caaaacgtcc cgggttgtcg ggatcctcca tgatgaattc ctgcgtgagc
     4921 gatgccgttc cgttcgcaat cgcaatgttg cgctgctgct tgacatactg cagaagctca
     4981 aagggaagct cgaggttgtt cgccacgggc cacacttttt cacgatcggt ttcatcgttg
     5041 ggcgaataat ttccagccgg agttacctgc agcggaatat catattcgta ataattatcc
     5101 gtctggtcgg aacccatgcg gatgaacccg acaagatcac cgttgttgag cggcgtcgtg
     5161 ctgcctgcac gagcctccgc gtgaatgaac atgcggatct ttttgtaaga gcgtacatcg
     5221 agattgttca ctgtcttgta gcacgcacgt gaatcgccgt cctcgagatc gcacaccttc
     5281 atggagagcg cctgctcgtt caggcggacc agcgtcgcgc tctgcacgtt ctgctcacgg
     5341 tcgatgccgg gcggaagaac atagttgaca ggcgtcttgt tgccgttctc ttcaatgttc
     5401 accgcggcaa gatcaaattc ggtgttgctc tggtcgttgg aaatatattc cccgggcgaa
     5461 agcagggagt aattgtattt tctccagtcg ctgcgcacaa gctcgaggcg cgcgaagcgg
     5521 cagataaccg gcttgtccac atccttgaaa taaatacgga tgaaacggat ggaattgaaa
     5581 ttttcgatgt tgccgacctt cgtagtgtac gactggatcg gaatgcggaa ctggtaccag
     5641 gtgatcggcc gcacacgtcc gtccttcgtg gtaaccgtcg tctggaaaac atccgtgata
     5701 taattgttcc cgttgttgtt cgggttgatg tcattcggcg tgagcttcac acggtattcg
     5761 taatagctct cattttcatt gagcgtatta tcacggttga tgtcttcctg gttgggaagc
     5821 gtcgttgcct gcgtcgggta ggattccgta tagccgtcat cattcggctg gccggtggcc
     5881 ggcgagttgc cgtccacccc gttgaacatt ttgtaacggt cgagaatgga aacctgcgca
     5941 ttgtcgtaat ccgttccccg gtaataatgg taattgtctc ccgccggatc tttcaccgcg
     6001 ttctggtaag ctacggaatt cgtgccccac cgcgcagaca cctggttcag gtaacttgca
     6061 aaataagtcg tctcattatc atccccgaga ccgtcgagac cgacgtcctg catcttgcgt
     6121 ttgtccgggt cgttatcgaa tgcattcaca atggactgca cgatcggcac acgtccccac
     6181 ggagtataat tgaccagcga tgtagccgta tccgatgtgg gaagtccgtt ctcaaagctg
     6241 ttgcgtgaat ctttcagcac gtcttccgaa atatttccaa gatcgaacac aagctcgccc
     6301 gtagtgttgc gctggttctg cggaacgtcg tcattgtaag gatccatcat ccagaactgg
     6361 atgaactcga tgttcgctgc ttcgaaatcg ttcgtctcga tgcggcgcat gatgccgccc
     6421 cagcgcgatg ccgggttcag gagatgaccg gaagagtcca gtcccgaaga aaatgacgtc
     6481 gggttacagt cgaagttgta cggtccgcga tcattcggat aatacgcaag gtcgagaacc
     6541 gacacgttca gcggctgccc gttgggtggc tgcttgttcg ggaaaacttc cgtctcaagc
     6601 acttcgcgca cacggtgatc cgactgatcg gccgtcgtga tgtagcctgg cttcaggtta
     6661 ttgtccggcc gcaaaaacag cggatcgatc acataccagc acaattttgc acggttgtat
     6721 cccgacatga cgctgtcctg cgtggcttcc ggaaaacgtt gcggctgtcc ctgcggaacg
     6781 gaagcgagcg accatccctg cggagagcgg atatctattg ccgactgcgt gccttcgaaa
     6841 tcgtcaatgt aggattgtcc cgttttaccg atgaccttgt tgtgaccggg atggagataa
     6901 gcgaattccc cctgcgctgt aatagacgac ggcgccttcg tatcgatcag cgggatcttg
     6961 tccaccatgc gcgtaagaat gggcgcgtcc gtccggtagt tgaaatccat tcccatgatc
     7021 gtgttgctca tcggttcgtc gccgacctgt atcttctgcg tgatcgggcg ctccgtcagg
     7081 ttcatcaccg ttccgccgat cgagaaatcc ttgttgaatt tgtaatcgag gtgcgttccc
     7141 caaagtgttt tcgactggat attgaaaagc gaattgcttt ccagcgagat cttgatcggc
     7201 acgccggaat tcaaaatgct ttcattgatg atcttgacgc ggcccagcgt atagtcgacc
     7261 gtgtaatcca cgttctcctg caatggcgca ccaccggccg tcaccacgac ggaaccctgc
     7321 ggaatgttca ctgcgttaag tgaaatatcc gaattcgatg acgactggta cgaacctttc
     7381 agcttaaagc ggtcatgctg cgtatcgacc gtctgcgcga atgtcttcgt ggaatcatac
     7441 agcgtctggt aacagtactt ggtaataaga cccggatcgc tcaattttcc cgcgagccat
     7501 ccaccgaacg gctgcactac ggggaagatc actcttccgt tcgaggagtt gatcgtcacg
     7561 ccgtcgatga agtcgaattg tccgtcgggt gaagcctgct gctgctggtt gagcttatcg
     7621 agattcaaca cctgcagcag gggtttcccg ttgatcggac cttccgggat gaagggaata
     7681 tccgttcccg tcgacgggtt gttgtaccac acctgcagct ggaaattctg cggattcacc
     7741 tggtatgcgc cgatggaata aacgttcttc atcatcaggt cccacgtctt gaagcgaacg
     7801 ccgttcacgt tggtgggact tgtcacgcgc ggaacgttat tcgtgctctt cagcattttc
     7861 acaaggatcg cattctgacc ggtaacgccg ccgtcggaaa actcgcctac ctggtatgtt
     7921 tttccattga gcgtatattg gtaagcgaca gcgacgactt catcatagtt gagcgcctgg
     7981 ttcaatgaaa tatacccgag ccgcgagttg accgaatact ccgtattcgc cagcttgcgc
     8041 gcattgttga cacgttcaaa gttgaccgag gacgtaaaat tataagccgt aataagcgga
     8101 gccagggaag ttgtcagcgg gacgcttatg tccctgacgc ttgaatacgt attggtaaga
     8161 tcatcgtaaa gcgtgttcag cttgttgtgt ggaacaggat cttcggttgt gttaatggta
     8221 atacctcccg gaacgaggtt gttttcaaga tcacccgttc cttcgccgat atcggagaat
     8281 gcaacgatgt tgcgcgtatt gtcaacggcg ccggtgcggt tcgtcaccca gacttccgtt
     8341 cgcacgatct gcacgccaga attgatcacc gggaaattgg cgagcgcctg gtcgtaatgg
     8401 ttgtaaaaat actgcgaaag gaaaaagtgt ttgttggcct cgtaattatc ggcggtcact
     8461 tcgaacgtgc tcacctgtgc gccgcccgtt acgtcgatct ccgatttttt tccgcgctcc
     8521 tgcgaaataa tgttcgtcat cgtcaggcgg ccgaactgga actgcgtctt gatgccgaaa
     8581 agcgtctggc tgccggtgat gagagatgtg ttcaacccga agctcacgtt accggcttcg
     8641 atctttttga tgatgtcgtc ctcgtagccg gtaaaatcca gcttcatctg gttttcccag
     8701 tcaaatgttg attccgtatt gtaattcgta gtcagcttca gcttgtcacc aatcttcccg
     8761 atgacgttca gctggatctt ctcatcgaaa tcgaacgtgg atatcctgcg ctgccgcacg
     8821 ggaagcgccg ggttgtccgt tttgttgatg ttgatcccga aaataagctc ggccgaaccc
     8881 tgcggcttga tgtcgatcga tccgccaccg aaaatatcct gtcccagctt tccgagcttg
     8941 atcgggggaa tgaggttttt attctgcccg cgctggttca tggtttcagc gtgctcacgc
     9001 tggcgccagt aatcgtgcac ggattttttc agatcgtaat attcgtattc atcgaatgtc
     9061 atgtacgtcg gcgggcggta atcgagccgg ttgccgatgc gctgctggac atcgtacgtg
     9121 ctggaatcgc cacgctgcgg attgtaggtg acggtcgttt tgatattgct gggatcgttc
     9181 agcttcagcg gcgcgtcatt ggtgtaatac atcgggtcga ggtacgactg atcctggaaa
     9241 ggatacggaa gcgtgtcgcc gccagcggga gaatctgttc cgcccaccag tccgcgatac
     9301 ggtgacgcaa tgcgcgcgtc gctgttcatg actgccgtaa gcagcacaac cgaagcgacg
     9361 acggcaattc cgaatttggc gaacaggttc aaaacgtatt ttatttcggg ttatagattc
     9421 tttaatgcga ttttcagcag gtgctcaact gaaacggctt tcccttcgga ggcgagcgct
     9481 ttgtcgatgg ctttttcagc cacattccgc gcaaagccga gcgtaaccag cgcggttaac
     9541 gcttcgtccc gcattttatt gtccccgccg aaagaaactg ctgaaattcc ttctgttccc
     9601 ttgcccattt ttcctttcag gtcgatgatg atccgctgcg ccgatttttc accgatccct
     9661 ttcaccgagc gaagcgccga aacatttccg gcatggatag cctgcacgat ctcggaagga
     9721 ctcatggatg aaaggatcat cctcgctgtt ccgggcccga cgcctgaaac ggaaatgagc
     9781 tggcggaaga tctcgcgctc ttcctcatcc gcaaaaccgt acagcaccat cgcatcttca
     9841 cgaacggcaa gatgcgcgaa caggcgaaaa tttccgctgt ccggtatcct ggaatatgtg
     9901 tgaagagaaa tattcatgag ataaccgacc ccgccgcaat ccactactgt atatgtcggc
     9961 gttttcattg ttatttttcc ttcgaaatga ttaaacatct gtttcagctc ctccgtgact
    10021 gggcatccac cacggcaatg attaccatat tcacgatctc acgtaccgag ctgccgagct
    10081 gcagcacgtg aacgggtttc ttcaggccga gaaggatcgg gccgatggct tccgaatccc
    10141 ccatcacctg gataagcttg tacgcgatat ttcctgaagc caggttcgga aaaataagcg
    10201 tgttcacttt ttttccgacc agctcgctga acgggaactg ctctttcagc aattcttcat
    10261 tcagcgcgaa attcgcctgc acatctccgt ctacaataag tcccgggtaa tcccggtgga
    10321 gaatttctac agccttcctt actttctcgg gaacttctcc cggggccgaa ccgaagttcg
    10381 tgtaagacaa aagcgcaatt ctcggaacga tattatactg ccggatcgcc tgggccgtca
    10441 gaacggttat gtcaaccagt tcttctgctg tgggatccac attcatagtc gtgtcggcaa
    10501 agaaaaagtt gcctgcccgt gtcgaaagaa tatacattcc tgccacacgg ttgacgcctt
    10561 cctgcacgcc tatgcaatgc agcgcgggaa ggatcggtgt ggcgtatttt cttgtaagcc
    10621 ccgagatcat cccgtcagcc attccggttt ccaccatcat ggctccgaaa tagttgcggt
    10681 cctgcatggc ctttttcgat tcatacaggg tatagccgcg gcgcttgcgg cgctggaaga
    10741 atatttctcc gaattccgtc cgctttgttt cctgcgaagg atcgcgggga tcgatgatgg
    10801 gcgtatcgcc gagatcgagg ttgttctctt ctatcagccg gttgatcttc gcgacgtcgc
    10861 ccagcaggat cggcctggca attccttcgt cgcgcaccac ctgcgctgct ttcagtattt
    10921 tataagtatc ggcctctgca aaaacaatgc gtttgggatt ttgcttggct ttcgtggcaa
    10981 gaccgcgcgt aagcttatta tcgcgcccga ggcgattgga caattcgatc gcgtacgcat
    11041 cccagtcggt gatcatcctg cgcgcaacac cggattcgat cgccgctttc gcgacggcaa
    11101 gtgaaacggt cgagatcagc cgcgaatcga tcggcttggg gatgatgtag ttttccccga
    11161 agctcaggtt acgcgtattg tacgcgtggt tgacatcatc gggaacaggt tccttggcca
    11221 aagccgcaat cgctttcgcg gcagcaagct tcatcgcttc attgatctgc gtggcacgaa
    11281 cgtcgagcgc accgcggaaa atgaacggga aaccgatcac gttgttcacc tggttcgggt
    11341 tatcggaacg tccggttgcc atgatgatat ccggccgtgc gcccgtcgca tcttcataag
    11401 atatttcggg atcgggattc gccagcgcaa acacaaccgg tttggccgcc atcgaccgga
    11461 ccatgtccgg cgtcaccacg tttcctttcg aaagcccgac aaaaacatcg gcgcctttca
    11521 tcgcttcggc cagcgtggaa accccggctg atttttttgc aaaaaacttt ttgtattcgt
    11581 ccaggtccgt acgatcggtg gtcagaacac ctttgctgtc gagcatggtg atgttctctt
    11641 ttttcgcacc aacagcgata tacatttttg cgcaggagat cgccgatgcg cccgcgccat
    11701 tgacaacgat ctttacttcg ccgatatttt ttcccgccag ttcgcaggca ttgatcatcg
    11761 cagccgaaga aataatggcc gtgccgtgct gatcatcatg catgacggga atggaaagct
    11821 cttctttcag cctgcgttcg atctcgaaac attcgggcgc tttaatgtcc tcgagattta
    11881 ttccgccgaa tgtcggcgag atcgctttca ctgtgctgac gaaggagtcg atatccgtgg
    11941 tgtccacttc gatgtcgaaa acatcgatgt ctgcgtagat cttgaaaagc atgcctttcc
    12001 cttccatcac cggtttcgac gcgagcgacc cgatgttgcc gagaccgaga actgcagtgc
    12061 cgttcgagat caccgcaaca agatttcctt tcgaggtata gcggtatgcg tcctccggat
    12121 ttttttcaat ctccaggcac ggctccgcaa caccgggtga ataggcaagt gtcaggtcga
    12181 actgtgagct gtagggtttg gtaggcagaa cctcgatctt tccgggtctt ccctgagaat
    12241 gataatccag ggcgtcctgc ttgcggaatt ttgccataat agaaaatatc ttttccggac
    12301 gtatccggtg agaagggcga aggtaatgaa atagtttctt aaaaaagccc ccttttcggt
    12361 ggaagccggc cctgttttca ttgtgaaaaa accggcaggc catccgggaa aaacatttat
    12421 aatcgtacgc ttctttccgg ttccacgatc atccgatttg tattatcttg ttcaactttg
    12481 acatcaggca aaatgtcggc ttaccgtaga atagttgtgt tcacaggagc aggagtcagc
    12541 gcagaaagcg gcatcagcac gtttcgcgac agcggcggac tgtgggaaaa atacgatata
    12601 cgcgatgtgg ccactcccga agcgtggatg aagaacccga accttgttct cgaattctat
    12661 aaccagcgcc gcaggcaggt gatggacgca aaacccaatc ctgcccacgt ttcccttgca
    12721 gagctggaaa agaaattcga cgttcatatc atcacccaga atgtggatga cctgcacgaa
    12781 cgcgccggct cgaaaaacgt gctgcacctg cacggtgaga tccggaaggc acgcagcacg
    12841 aagaaccctg agcttattta tcctgtaaaa ggatgggaga tcaaagccgg cgacaaatgc
    12901 gagctcggct cccagctccg tccgcacatc gtctggttcg gggaatttgt tccggaaatg
    12961 gaccgcgccc aggagattgc agcgggcgcc gaattattca tcatcatcgg aacgtctctc
    13021 aacgtttatc ccgcggcagg catcattcat ttcgtcaaac gcgatgtgcc gatcatcatc
    13081 atcgatccga acgaaatcaa caacccgggc ataaaaggga ttacattcat ccgcagcaat
    13141 gccggcgcgg gcgtccccgt
//
LOCUS       DASTSF010000115         2804 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1937425,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000115 DASTSF010000000
VERSION     DASTSF010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2804
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1937425"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(153..1502)
                     /locus_tag="VFU15_03440"
     CDS             complement(153..1502)
                     /locus_tag="VFU15_03440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716856.1"
                     /translation="MRRCLIIESLPYHWEVLVAWVHMLKDLGIDAEIAVGETSGHRET
                     VALLGLEPGKVHDVRNLGSLPPARFDFVLLNTLVHDGYFFSQAPVPRPDLELLDRLQL
                     PSVCVIHEPVQWVEKLVVHSFIESRDGSEKIINLLADGSSVRENGFWSAERWTIDGDK
                     LMLPCDGALLEFRSSDRGQTYRCVDDPGILLRTRVTPVEDLHGHLGRKQHAVITLTAG
                     GAAKLGEVYTGVEWILPFKIDERHPARSDGPIAFAGTIDYDRKAIPSLLAAAGSLEPG
                     ERILVIGGSRSADFENDRFVKTFRRQIAELGIGEKFLFTGYLPYDQFIEKIKRCRFII
                     PLVDDYVDSGAYLVKLPAAVPLSLGLGVPMIMSKTVSGKFDVDALISYPGQDLAGGLD
                     ACRRMTSENYAQMLQTLDDTAKRIYAGNLQKLGAIIDRITGPGSGKLKEPATKSTSC"
     gene            complement(1511..>2804)
                     /locus_tag="VFU15_03445"
     CDS             complement(1511..>2804)
                     /locus_tag="VFU15_03445"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HEU4716857.1"
                     /translation="RFLRCIAHGDEERAANTGVEDIGGVMRANEGTSRLNRERSERNV
                     REGNLVLNSGDFGTGVVYRNFSPAPVLVSPFRYRRISFCTASMGRLHHLLETLPQNIE
                     NNRSYPDLEFVLLDYNSQDGLEEWAKQHLAGHIEDGRLVYYKYTGRKYFDRSHARNLA
                     ARLASGEIICNIDADNFTGVDFAHYINERFSHSSSIFLRPDFDGAHSRLRDAFGRLCV
                     MKSHFHAVEGYDEKFIDYGYEDNDLCSRLEKNGIEPALIEDQRFLRYIEHNNFSRMEN
                     GMLAHRLALMLEGRYADQEWDTLVCLFDDGEFFCSGPLLNSLPGEGRWEKNSSGILLQ
                     SRSGHVCRLFNYSQGRFSLETGPDSLFVLKESNNMSRFMQAYFNHSLIRNERRFRQNR
                     ESRSGHINERNYGLATVFRNFKETPIQLKKLAPLERVK"
BASE COUNT          654 a          756 c          707 g          687 t
ORIGIN      
        1 aaagttcgac cttcctccac cctcccccaa aaagctattg atattcaaaa tctttttgca
       61 ctgaaatctg accggcattc aacctatgat ttctaaaaca accggcgatc aaaaacatct
      121 gcaacattat tcgcgttgat acgggagtga ttctaacagc tcgtgctttt tgtcgcgggt
      181 tctttcaatt taccggatcc agggcctgtt attctgtcta tgatcgctcc gagcttttgc
      241 aggttgccgg cgtaaatgcg ttttgcggta tcgtccagcg tttgcagcat ctgtgcataa
      301 ttttcactgg tcatgcgcct gcacgcgtcg agtccgcctg ccaggtcctg gccgggatag
      361 gaaataagcg catccacgtc aaacttaccg gacacggttt tgctcatgat catcggcact
      421 cccagtccga gcgacagggg aactgccgca ggaagcttga cgaggtatgc gccggaatca
      481 acgtagtcgt cgactaacgg gatgataaac cggcaccttt ttattttttc gatgaactga
      541 tcgtaaggaa ggtaaccggt gaagagaaat ttttccccta tacccaattc ggctatttgc
      601 cgcctgaatg tttttacaaa ccggtcgttt tcgaaatcgg cgctgcgcga accgccaatg
      661 accaggatgc gttcgcccgg ttcgagcgac ccggcagcgg cgagcagcga gggaatggct
      721 ttcctgtcgt aatcaattgt gccggcgaag gcgatcggtc catcgcttcg cgcggggtgg
      781 cgctcatcta ttttgaaagg gagaatccat tccactcccg tatacacttc tcccagtttg
      841 gcggctcctc ctgccgtaag cgtaatcacc gcgtgctgct tcctgcccag gtgtccgtgg
      901 agatcttcga cgggagttac gcgcgtgcgc aggagaattc ccggatcatc cacgcaccgg
      961 taggtttgtc cccgatcgga agaacggaat tcaagtaagg ccccgtcgca gggcagcatt
     1021 aatttatctc cgtcaatggt ccatctttcg gcagaccaga acccgttttc gcgtacactg
     1081 gatccatcgg caaggagatt aattattttt tcagagccgt cccgtgattc gataaaagaa
     1141 tgaacgacca gcttttccac ccactgcaca ggttcgtgaa tcacgcagac cgatggaagc
     1201 tgcaacctgt caagcagctc caggtcgggc cgcgggacag gcgcctgtga aaaaaaataa
     1261 ccatcgtgca ccaatgtatt gagcagcaca aaatcaaacc gggcgggcgg cagcgaaccc
     1321 aggttgcgga catcatgaac cttgccgggc tcaagcccga gcaaagcgac cgtctcccgg
     1381 tgcccggatg tttcaccgac ggcgatctcg gcatctatgc ccaaatcttt cagcatgtgc
     1441 acccaggcga ccagcacttc ccaatggtaa ggaagtgatt cgatgatcag gcagcgtctc
     1501 acctgctttt tcattttaca cgctccagcg gcgcaagctt ttttaactga ataggcgttt
     1561 ccttgaaatt ccggaagaca gtggccagcc cgtaatttct ttcatttata tgcccggacc
     1621 tgctttcccg gttttgcctg aaccttcgct cattccggat cagcgaatga ttgaagtagg
     1681 cctgcataaa acgcgacatg ttgtttgatt ccttcaaaac aaaaagagaa tccggccccg
     1741 tctccagtga aaaccgtccc tgcgaataat tgaaaagccg gcagacgtga cccgacctgc
     1801 tttgcagaag tatgcccgaa ctgttctttt cccatctgcc ttcgccgggc aaactattga
     1861 gcaggggtcc tgaacaaaag aattctccgt catcgaacag gcagacaagc gtgtcccatt
     1921 cctggtctgc gtaccggcct tcgagcataa gcgcaagacg atgggcgagc attccgtttt
     1981 ccatccgtga aaaattgttg tgttcgatat accggagaaa acgctgatcc tcgatcagcg
     2041 ccggttcgat gccgtttttt tcaagcctgc tgcaaaggtc attgtcttcg taaccgtaat
     2101 cgatgaactt ttcgtcgtat ccttcgacag catgaaaatg ggatttcatg acgcagagtc
     2161 ttccgaatgc atcgcgaagc ctgctgtggg cgccgtcaaa atcgggtctc aggaaaatag
     2221 atgaggaatg cgaaaatcgt tcgtttatgt aatgggcgaa atcgacaccg gtaaaattgt
     2281 cggcatcgat attacagatg atctcgccgc tggcaagacg ggcagcgagg ttcctggcgt
     2341 gactgcggtc gaaatatttc cggccggtat acttgtaata aaccaggcgg ccgtcttcaa
     2401 tgtgtcctgc caggtgctgt ttcgcccatt cctccaaacc atcctgggaa ttataatcga
     2461 gaagaacgaa ctcgaggtcg ggatagctcc tgttattttc aatgttttgc ggaagggtct
     2521 cgagaagatg atgcagccgg cccatgcttg cagtgcagaa tgagatcctg cggtatctga
     2581 aaggacttac cagtaccggt gcaggagaaa aattccggta gacgacaccg gtgccgaaat
     2641 cgccggaatt cagcacgagg ttgccttccc tgacattgcg ttcgctgcgt tcgcggttga
     2701 gcctggaagt tccttcgttt gccctcatta cgccgccgat atcttcaaca ccggtattcg
     2761 ccgcacgttc ttcgtcgccg tgcgctatgc acctgagaaa cctg
//
LOCUS       DASTSF010000116         2397 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1042518,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000116 DASTSF010000000
VERSION     DASTSF010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2397
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1042518"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="VFU15_03450"
     CDS             <1..197
                     /locus_tag="VFU15_03450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716858.1"
                     /translation="TAAFYYAGALCAIFGMEMLISFGASFLKKFITRRFLIWVDRTVG
                     ALFIGFSVVLIFPVVKTLFR"
     gene            complement(387..1769)
                     /locus_tag="VFU15_03455"
     CDS             complement(387..1769)
                     /locus_tag="VFU15_03455"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEU4716859.1"
                     /translation="MTERKKYNPVRPITAKGKLLTGIVRALYGARRTFGGKDWEYVLQ
                     GHHEDPLKMNAEQRLYLGHKYYYRALLNADENSGLEKHFRENAGTLQVPGDFRAHSTV
                     TLSAGGDLMPYFCITEKTCADLWKETGDFFFGADIVTANLETPVDTGKRPYFVPEVML
                     SNMYFNGGEEMFSVFSGMGKYRGYDFLSTANNHSLDMGEDGVHATLSFLEKKNILSAG
                     TASSEKMRDEVKMITRNGVRIAFLSFTFSLNREVCPPGKEYLVNHLNLNEDGADISAI
                     VSQAKNARAAGADFLVAALHMGCAYQPYPSQTIVDNMRRICRETGIDVVLGGHPHNPQ
                     PMEFIEVTDPFSGEKKNSFVIYSFGDFVAYDIFKWCRLPLMMKFSISKGTCGGKEKTI
                     LTGVEAKFVYNRSVTKRGNVEKLEFLDFSMLRNDDSVLDDAASKEEFRELRWFAETFL
                     LPGNLEKFLV"
     gene            1844..>2397
                     /locus_tag="VFU15_03460"
     CDS             1844..>2397
                     /locus_tag="VFU15_03460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716860.1"
                     /translation="MFVIRHFTEVHIGRLVVQDPVTVLTNILLFLAGMWGFSRVRPLA
                     FPGAKSWSMFFFMLGLASFTGIFVHGFAHYMTDSLLFEVWVIMGLVQNVGISFAQMAT
                     AQEYFPKYTGPILLFVIAQYLVLGFIFVYYRTYEASKAHVALGMAPVMLWNLYRYFKG
                     EKKARFIFLGILVSSLGGAVHTLQ"
BASE COUNT          497 a          637 c          624 g          639 t
ORIGIN      
        1 gaacggcggc attttattac gccggtgcgc tctgcgccat tttcgggatg gaaatgctca
       61 tctctttcgg cgcgtccttc ctcaaaaaat tcatcacgcg caggttcctc atctgggtgg
      121 accgtaccgt aggcgcgctg ttcatcgggt tttccgtggt gctcattttc ccggtggtga
      181 aaacgttgtt ccggtgaatg aaacccggcg gatatgcaga tctcttcgtg aacttccttc
      241 gtggtaaaaa cgactttagc ttatcgcagg tcattcatcg ccctgacttt ccaaccgtcc
      301 aacccttcag cctttcaacc ttccaacttt gaaacggaat tctcttcgtt ttccttcgtg
      361 gtgaaacgtt cagcacgttc gaaaggtcac accaggaact tctccaggtt tcccggcagc
      421 aggaacgttt ccgcaaacca tctgagctcg cggaattctt cttttgaagc cgcgtcgtca
      481 agcacagaat cgtcattcct cagcattgaa aaatcgagga actccagctt ttccacgttc
      541 cctcttttcg taacggaacg gttgtaaacg aatttcgctt cgacaccggt gaggatcgtt
      601 ttttcctttc ccccgcaagt tcctttcgaa atggaaaatt tcatcatcag cggaagacgg
      661 caccatttga aaatatcgta cgccacgaaa tcgccgaaag aataaatgac aaaggaattt
      721 ttcttttcac cggaaaaagg atccgtcact tcgatgaatt ccatcggctg cgggttgtgc
      781 ggatgcccgc ccagcacgac atcgatcccc gtttcccggc agatgcggcg catgttgtcc
      841 acgatcgtct gcgaaggata tggctggtag gcgcaaccca tgtgcagtgc agcgacgagg
      901 aaatcggcgc cggcagcgcg tgcattcttt gcctgtgaaa caatggcgga aatgtctgcg
      961 ccgtcttcgt tcaggttgag gtgattgaca aggtattctt ttccgggcgg acaaacttcc
     1021 cggttcagcg aaaacgtaaa cgaaaggaac gcgatgcgca cgccgttgcg cgtgatcatt
     1081 ttcacttcgt cgcgcatttt ttccgaagag gccgtgcctg cggaaaggat gttctttttt
     1141 tcgaggaagg aaagcgtggc gtgaacgccg tcttcgccca tgtccagcga atggttgttg
     1201 gccgtggaaa gaaaatcgta cccgcggtat tttcccattc ctgaaaaaac gctgaacatt
     1261 tcttcgcctc cgttgaagta catgttactc agcattactt ccggcacgaa atacgggcgc
     1321 ttccccgtgt caacaggcgt ttcaagattg gccgtgacga tgtcggcgcc gaagaaaaaa
     1381 tcgccggtct ccttccagag atcagcgcat gttttttccg tgatgcagaa atacggcatg
     1441 aggtcgccgc cggcggaaag cgtgaccgtg gaatgcgccc ggaaatcccc cggcacctgc
     1501 agcgttccgg cattttcccg gaaatgtttt tcaaggccgg aattctcgtc ggcgttcagc
     1561 aatgcgcggt aataatattt gtgcccgagg tacaaacgct gttctgcatt catcttcagc
     1621 ggatcttcat gatgtccctg cagcacatat tcccagtctt ttccgccaaa cgtgcgtctt
     1681 gctccataca gcgcacgcac gatgcccgtg agcagctttc ccttcgcggt tatcggccgc
     1741 accgggttgt attttttccg ttcagtcaca gcggcaaagg tagaaggtat tcgcgggttt
     1801 gcagtgaaca cgggccttgc ctgcttttcg gtatttttgc gcgatgttcg tgatccggca
     1861 ttttacggaa gtccatatcg gccggcttgt ggtgcaggac ccggtgaccg tgctgacaaa
     1921 tatcctgctg ttcttggccg ggatgtgggg tttttcccgt gtgcgtccgc tcgcatttcc
     1981 cggggcaaaa tcatggagca tgtttttttt catgctcggg ctggcgtcgt tcaccgggat
     2041 cttcgtgcat gggtttgcac attacatgac ggacagtctt cttttcgaag tgtgggtgat
     2101 catgggactg gtgcagaacg tgggaatttc attcgcgcag atggcgacgg cccaggaata
     2161 ttttccgaaa tacacgggcc ccattctcct tttcgtgatc gcccaatacc tcgtgctcgg
     2221 gtttatattc gtgtattacc ggacgtacga agcgtcaaaa gcgcacgtcg cgctcggcat
     2281 ggcgccggtc atgctctgga acctctaccg gtatttcaaa ggagaaaaga aagcgcggtt
     2341 catttttctc gggatacttg tttcttccct cggcggtgct gtgcatacgt tgcagtg
//
LOCUS       DASTSF010000117         2084 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_371424,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000117 DASTSF010000000
VERSION     DASTSF010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2084
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_371424"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..341
                     /locus_tag="VFU15_03465"
     CDS             <1..341
                     /locus_tag="VFU15_03465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HEU4716861.1"
                     /translation="PVFRIKVPFETRVQRLVNDYGKFPAAVLAEATARIRKRLGGLAT
                     KQALEAIGNGQLDETVRITLRYYDRAYDHPHKERNYSNVFFIETDSGDPAHNAELILE
                     EARRQKNTLS"
     gene            360..1403
                     /gene="selD"
                     /locus_tag="VFU15_03470"
     CDS             360..1403
                     /gene="selD"
                     /locus_tag="VFU15_03470"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019948811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HEU4716862.1"
                     /translation="MHEVKDKTRLTQYSHGAGCGCKIAPAVLDGILKTGLKPEHNPLL
                     LVGYDKKDDAAVFDLGNGKAVISTTDFFMPIVDDAFDFGRIASVNAISDVYAMGGKPL
                     LAIAILGWPVNTVPTELASKVLEGARAVCAQAGIPLAGGHSIDNPEPLFGLAVTGEVD
                     IPHLKQNSTATEGCSLFLTKPLGVGILTTAQKKGIVRDEDFSRAVNSMLTLNDAGATF
                     GKLDYVKAMTDVTGFGLLGHLGEMADGSNLSAIVEFEKVPKIPGLDFYIEQKSIPGGT
                     KRNWDSYGNGITGVDDYRRAILCDPQTSGGLLVAVEKGKEKDFTEAATATGLHLSPFG
                     HLVARKEERIIVH"
     gene            1420..>2084
                     /locus_tag="VFU15_03475"
     CDS             1420..>2084
                     /locus_tag="VFU15_03475"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEU4716863.1"
                     /translation="MIKAVIVDDSPEARESLSADLKRWCPQVALIGEAGSMKEGLNVL
                     RTLKPDLVFLDIDLGDGDGFGLLENLGETNAKVIFTTGLDSHGIRAIKFSALDYLLKP
                     VDPDELVKAVAKLENENEKHSLADNLRFLAESLKTGTPGSPKRIALNSADRVQVVNVQ
                     DIIRCESERNYTTFFLKGGKQIVVTRTLKEYEEMLEPNGFVRVHHSHLINLEHLKEFI
                     KSDG"
BASE COUNT          533 a          562 c          561 g          428 t
ORIGIN      
        1 cgcccgtatt ccgcataaaa gttcccttcg aaacgcgcgt tcagcgtttg gtgaatgatt
       61 acgggaaatt tcccgcagcc gttctcgccg aagctacggc acggatacgg aaacgtctcg
      121 gcggacttgc cacgaagcag gcgctggaag cgatcgggaa cgggcagctg gatgaaaccg
      181 tccgcatcac gctgcgttat tatgaccgcg cttacgatca tccgcataag gaaaggaatt
      241 acagcaatgt ttttttcatt gaaacggact ccggcgaccc ggcgcacaat gcggaactca
      301 tccttgaaga agcgcgcagg caaaaaaaca cgttaagtta aattcactat tttcaacgca
      361 tgcacgaagt aaaagacaag acacgtctca ctcaatacag tcacggagcc gggtgcggat
      421 gcaagatcgc tccggcggtg ctcgacggca tcctgaaaac cgggttgaaa ccggaacata
      481 atcccctgct tctcgtcggt tacgataaaa aagatgacgc tgcggttttc gatctcggga
      541 acggaaaagc ggtcataagc acgacggatt tttttatgcc cattgtcgac gatgcgttcg
      601 atttcggccg cattgcttcc gtgaacgcga tcagcgatgt ttatgccatg ggcggaaaac
      661 cgctcctcgc catcgccatt ctaggctggc cggtgaacac tgttcccacg gagctggcgt
      721 caaaagtgct cgaaggcgcg cgcgccgtgt gtgcgcaggc gggaattccg ctcgcgggcg
      781 gtcacagcat cgacaaccct gaacctttgt tcggtctcgc cgtcaccggc gaagtggaca
      841 ttccgcacct gaagcagaat tccacggcga cggaaggctg ctcgcttttc ctgaccaaac
      901 ctctcggcgt cggtattctc accaccgcac agaaaaaagg gatcgtgcgc gacgaagatt
      961 tttcccgcgc cgtgaattcc atgcttacgc tgaacgatgc gggcgcgaca ttcggaaaac
     1021 tggattacgt aaaagcaatg acggacgtaa ccggtttcgg actgctcggt catctgggtg
     1081 aaatggccga cggcagcaac ctttccgcga tcgtggaatt tgaaaaggtc ccgaaaatcc
     1141 cgggactcga tttttacatt gagcagaaat caattcccgg cggaacaaaa cggaactggg
     1201 acagttacgg gaacggcatc acgggcgtgg acgattaccg tcgcgccatt ctctgcgatc
     1261 cgcagaccag cggcggactt cttgttgccg ttgaaaaagg aaaagagaaa gatttcacgg
     1321 aggctgcaac ggccaccggc ctgcatcttt ccccgttcgg tcatcttgtt gcgcgaaagg
     1381 aagaacgcat tattgtacac tgatttcaaa aaaagaagta tgatcaaagc agtaatcgtc
     1441 gacgattccc ccgaagcacg cgaaagtctt tccgctgatc tcaaacgctg gtgcccgcag
     1501 gtagctctta tcggcgaggc cggcagcatg aaggaaggat tgaatgtgct ccgcacactg
     1561 aaaccggatc ttgttttcct cgacattgat ctcggcgacg gggacggttt cggattgctg
     1621 gaaaatctag gtgaaacaaa tgcgaaagtg attttcacca ccggcctcga cagccacggc
     1681 atacgcgcga taaaattcag cgcgctcgat tacctgctga aacccgtcga tcctgacgag
     1741 ctggtgaaag cggtggcgaa actcgagaat gagaatgaaa aacactcgct ggcggacaat
     1801 ctccgttttc ttgccgaatc actgaagacg ggaacgcccg gttcgcccaa gcgcatcgcg
     1861 ctcaacagcg ccgatcgtgt gcaggtggtg aacgtgcagg atattatccg ctgcgaatcc
     1921 gaacgcaact acaccacgtt tttcctcaaa ggcggaaagc agatcgttgt cacacgcacg
     1981 ctgaaagaat atgaggagat gctcgagccg aacggttttg tacgcgtgca ccactcgcac
     2041 ctcatcaacc tcgaacacct gaaagaattc atcaaaagtg acgg
//
LOCUS       DASTSF010000118         6264 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1934983,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000118 DASTSF010000000
VERSION     DASTSF010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 6264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6264
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1934983"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(77..442)
                     /locus_tag="VFU15_03480"
     CDS             complement(77..442)
                     /locus_tag="VFU15_03480"
                     /inference="COORDINATES: protein motif:HMM:NF009150.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HEU4716864.1"
                     /translation="MAQNHETGKWGEEEAQGFLLSKGYEVLDVNWKFLHLEIDIVAKI
                     GEKLVIVEVKTRGTDAYGDPEMFVSKTKQRKLIRAANLYLDQKKLENEVRFDVIGIIR
                     NNGHFRLRHVQDAFAPFGG"
     gene            593..1228
                     /locus_tag="VFU15_03485"
     CDS             593..1228
                     /locus_tag="VFU15_03485"
                     /inference="COORDINATES: protein motif:HMM:NF025729.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4412 domain-containing protein"
                     /protein_id="HEU4716865.1"
                     /translation="MLRKLFFFLLLISVSARISAQNFEGIIEFKKQTGTEVLNYVYYV
                     KGDRVRIDEFTPGTRTVSGSFILDVNKKTMIYLNHDRKLWGTRQPPAADPVAPAGCIA
                     SATKNSKTLFGYKCTENIVKNVSDSTQISYWVANGHFAFFTPMMKLLNRQEKFSTYFF
                     AMTLKDGAFPFLAVESDLNGKEISRLEVTRFEQKTLDAGLFSTPKDYTEVK"
     gene            complement(1235..2152)
                     /locus_tag="VFU15_03490"
     CDS             complement(1235..2152)
                     /locus_tag="VFU15_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008255628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEU4716866.1"
                     /translation="MKTKEITLPPFLRPGDTVGICAVARKVTKEEIEPAVRKLEEWGF
                     YVELGQHLFGAENQYSGSDEERASDLQAFLDDPSVKAVISARGGYGTMRIIDRLDFTK
                     FREQPKWITGYSDITVLHSHIHTNFSIATMHATMPLNFAKDPFSTETLREGWCGEPLS
                     YKSIDTVNAENRSGFAAGQLTGGNLSLLYALQASASDIDTDGKILFLEDLDEYLYHID
                     RMMLSLKRAGKFDGLAGLIVGGMNDMKDNTVPFGKSAEKIIAEHVREFSFPVCYGFPA
                     GHDVKNYALALGRTIHLDVNGPETVLTFV"
     gene            2285..4387
                     /gene="metG"
                     /locus_tag="VFU15_03495"
     CDS             2285..4387
                     /gene="metG"
                     /locus_tag="VFU15_03495"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013926017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEU4716867.1"
                     /translation="MHDPKRYTVTAALIYANGPIHIGHLAGCYLPSDIYVRYLRSNGK
                     DVLFVSGTDEHGVPITLRAKKEGLTPQQVVDKYYGVIRKAFYDFGIEFDIYSRTSRPA
                     HHAVASDFFKTLYDKNIFTEQTTEQYYDEEAKQFLADRYIVGTCPNCKNENAYGDQCE
                     KCGTSLSPDELINPHSTLSGSRPTKRPTKNWFLPLDKLQPQLQDYIDSHKEWKPNVFG
                     QCQSWLKDGLHPRAMTRDLDWGVKVPLPGTEGKVLYVWFDAPIGYITASKEWFTQPET
                     RAQLTYNGKPKSEGWEIYWKDKETKLVHFIGKDNIVFHCLIFPAMLMAHGGYILPDNV
                     PANEFLNLEGNKISTSRNWAIWLHEYLEEMPGRQDELRYVLTAIAPETSDGDFSWTDY
                     EARVNNELAAVFSNFVHRTMVLTHKSCGGKVPEAKQYTKEDLHVLGEISKFPEKIGTA
                     IGHYRFREAQQELINLARVGNKYLSDNEPWKNPDAERVNTILNVSLQICANLGILCEP
                     FLPFTAAKLRGMLNMGVMSWSNAKNTNLLTAGHALNQPVMLFAKVDKELLKMQTEKLE
                     KIKKQQEEENSRMGNSQPASLEPQKATTTFDEFSKMDIRTGTIIAAEKMPKSDKLLKL
                     VVDTGLDQRTVLSGIAKYYSPENIIGQQVTILVNLAPRKMMGVESNGMILMAENEKGE
                     LSFVAPTEKNINNGSTVK"
     gene            4499..4570
                     /locus_tag="VFU15_03500"
     tRNA            4499..4570
                     /locus_tag="VFU15_03500"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4532..4534,aa:Arg,seq:cct)
     gene            4697..>6264
                     /locus_tag="VFU15_03505"
     CDS             4697..>6264
                     /locus_tag="VFU15_03505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716868.1"
                     /translation="MTKFLGIEGLNKVKSLFNAIAVIVAPFSIATALVLWIENKNNAA
                     IAVLWWHNSYFIWLLLATIFLMLSTILYLSLSSDKSNTIRLGAKTEKKKTGSMKNILR
                     VAVILGVVNFFLALFFYGTTKFNSEQTTGILGITYSNSEFQAALQSFLRGKCGENELQ
                     FTDNQRAIEDIKYQDALFATYFQEGLLYYGHCTKVDSLLLIDCTLKWHGQKHTDIDTN
                     QTIPSSVYRNLDDYSLKIRTNAKIISDFILGYYYVHYSDSSKTAIKYFQKIINDSIFQ
                     AAIRSRPKDEDLQAFNKDLQMFQKYAELLVTDQSKQSAVVPCNNGELWQYSLGGKLSS
                     YQGLVKSTQEFSVDFYFDNYNTEYNSYSGATLVAAINSMSNPAFIISAITLQTAVLTP
                     EERDSLSERQRESLEQNRTASVRDFIWRELLPIKKEIDIGDCQVSHDIAPTMPWPVFQ
                     EAISKTNVEDKDVILSVLSKYPDGEKREAEIRNMAATYRGLPQILYTQAQIHVVIKGV
                     ELWPSSTIVRNALQ"
BASE COUNT         1679 a         1533 c         1522 g         1530 t
ORIGIN      
        1 gaaacgatcg acgcacaacc cggaatttcc attgcggata cggtcatccg ttccggtgag
       61 gcaggcacga acgttctcat ccgccgaacg gagcaaacgc atcctgcacg tgacgcaggc
      121 ggaaatgccc gttgttcctg atgatgccga tcacatcgaa gcgcacctcg ttctccagct
      181 ttttctgatc gaggtaaagg ttggcggcgc ggatcagctt gcgctgcttg gttttggaga
      241 cgaacatctc cgggtcgccg taagcgtcgg tgccgcgtgt tttcacctcg acgatgacaa
      301 gcttctctcc tattttcgcg acaatgtcga tctcgaggtg aaggaatttc cagttcacat
      361 ccagcacctc gtatcctttg gaaagcagga agccctgcgc ctcttcttcg ccccactttc
      421 cggtttcgtg attctgagcc atgggccgaa aataaaacag cgcaactcga ttagtcgttt
      481 gaaaatgaat aaattgaacg catacttatt ttccggaacg ctttttgaat gattttctaa
      541 taagaaccat cctcaacttt tcgtagattt aataagtaaa atctccttta cgatgctcag
      601 aaagctgttc ttttttctcc tgctcatttc cgtttctgcc aggatatcgg cgcagaattt
      661 cgaaggaatc attgaattca aaaaacagac gggaaccgaa gtcctcaact acgtgtatta
      721 cgtgaaaggc gacagggtgc gcatcgacga gttcacgccc ggaacaagaa cagtttccgg
      781 cagctttatt ctcgacgtga acaagaaaac gatgatctac ctcaaccacg accgcaagct
      841 gtggggaaca cgccagccgc cagccgcgga cccggtcgct cctgcgggct gcatcgcttc
      901 cgccacgaaa aattccaaaa cgcttttcgg ctacaagtgc accgagaaca tcgtgaagaa
      961 cgtttccgac agcacgcaga tcagctactg ggtggcgaac ggtcatttcg cttttttcac
     1021 gccgatgatg aagctgctca accgccagga aaaattctcg acttactttt tcgcgatgac
     1081 gctgaaagat ggcgcattcc cttttcttgc cgttgaatcc gacctgaacg gaaaagagat
     1141 cagccgcctc gaagtgacgc gtttcgagca gaagacactc gacgccgggc ttttcagcac
     1201 acccaaagat tacacggagg tcaagtagct tcgttcacac gaacgtcagc acggtttcag
     1261 gtccgttcac gtcgaggtga atggtccttc ccagcgcaag cgcataattt tttacatcat
     1321 gtcccgcggg aaagccgtaa caaaccggga atgagaattc acgcacatgt tcagcgataa
     1381 ttttctcagc tgattttccg aacggaaccg tattgtcttt catgtcgttc atcccgccga
     1441 caatgagtcc tgcaaggccg tcgaattttc ccgcgcgttt caggctgagc atcatgcggt
     1501 cgatgtggta gagatattca tcgaggtctt cgaggaaaag tattttcccg tccgtgtcga
     1561 tatcggaagc ggacgcctgc aatgcgtaga gcagggaaag atttcctccg gtgagctgcc
     1621 ccgcggcaaa cccgctgcgg ttttccgcat tcaccgtatc gatgcttttg tacgaaagcg
     1681 gttccccgca ccagccttcg cgcagcgttt ccgtcgagaa cggatctttc gcaaaattga
     1741 gcggcatcgt cgcgtgcatg gtcgctatgc tgaagttcgt gtggatgtgg ctgtgcagga
     1801 ccgtgatatc gctgtagccc gtgatccatt tcggctgttc acggaatttc gtgaaatcga
     1861 gacggtcgat gatgcgcatc gttccgtagc cgccgcgtgc gctgatgacc gctttgacgg
     1921 aaggatcgtc gaggaacgcc tggagatcgg aagcgcgttc ttcgtcgctg ccggaatact
     1981 ggttttccgc tccgaaaaga tgctgtccca gctcgacgta aaacccccac tcttccagct
     2041 tgcggacagc gggctcaatt tcctcctttg tcacttttcg cgccaccgcg cagatgccga
     2101 cggtatcacc gggacgaaga aagggaggta aggtgatctc ttttgttttc atgagccttg
     2161 tttttcacac cggacgcagc ttcgaattcc gctgcaggtt tgaaaaggcc tgcgcgaaat
     2221 agtatcttcg tgcctcgttt ttacagcgta aaagtacaat ccgccatccg attttaacgg
     2281 aattatgcac gatccgaaac gatataccgt caccgccgcg ctgatctacg ccaacgggcc
     2341 tattcacatc ggtcacctgg ccggctgcta cctgccttcg gatatttatg tccgttacct
     2401 gcgcagcaac ggcaaagatg tgctgttcgt gagcggaacc gacgagcatg gtgtgccgat
     2461 cacgcttcgc gcaaaaaaag aagggctgac gccgcagcag gtggtggaca aatattacgg
     2521 cgttatccgc aaagcctttt acgatttcgg aatcgagttc gatatttatt cgcgaacgag
     2581 ccgtcccgct catcatgcag tggccagtga ttttttcaaa acgctgtacg ataaaaacat
     2641 tttcaccgag cagacgacgg agcagtatta cgacgaagag gcgaaacaat ttctcgccga
     2701 ccgctacatc gtcggtacgt gcccgaactg caaaaacgaa aacgcctacg gcgaccagtg
     2761 cgagaaatgc ggcacctctc tttcacccga cgagctgatc aacccgcatt ccacgctgtc
     2821 cggttcccgt ccgacaaaac gtccgacgaa aaactggttc cttccgctcg acaagctgca
     2881 gccgcagctc caggattaca tcgacagcca caaagaatgg aaaccgaacg ttttcgggca
     2941 atgccagtcg tggctgaaag acgggcttca cccgcgcgcg atgacacgcg acctcgactg
     3001 gggcgtgaaa gtcccgctgc cgggcacgga aggaaaagtg ctgtacgtgt ggttcgatgc
     3061 gccgatcggc tacatcactg cgagcaagga atggttcacg caaccggaaa cacgcgcgca
     3121 gctgacctac aacggcaagc cgaaaagcga aggctgggaa atttactgga aggacaaaga
     3181 gacgaagctt gtgcatttca tcgggaagga caacatcgtt ttccattgcc tcattttccc
     3241 ggcgatgctc atggcgcacg gcggttacat tcttccggac aacgttcctg ccaatgaatt
     3301 cctgaatctc gaaggaaata aaatctccac gtcgcgcaac tgggcgatct ggctgcacga
     3361 atacctggaa gaaatgcccg gccgccagga cgagctgcgt tacgtgctca cggccatcgc
     3421 gcccgaaacc agcgacggcg atttctcctg gaccgattac gaggcgcgtg tgaacaacga
     3481 gctcgcagcg gtgttcagca atttcgttca ccgtacgatg gtgctcacac acaaaagctg
     3541 cggaggaaaa gttcccgaag cgaagcaata cacaaaggaa gatctccatg tgctcggtga
     3601 aatttcgaag ttccccgaga agatcggcac cgcgatcggg cattaccgtt tccgcgaagc
     3661 gcagcaggaa ctgataaacc tcgcgcgcgt cggcaacaaa tatttgtcag ataacgagcc
     3721 gtggaaaaac ccggatgcgg aacgcgtgaa tacgatactc aacgtttcgc tgcagatctg
     3781 cgcgaacctc ggcattctct gcgaaccgtt cctgccgttc accgcggcga agctgcgcgg
     3841 catgctcaac atgggcgtga tgagctggtc gaacgcgaag aacacgaacc tgctcacagc
     3901 gggccacgcg ctgaaccagc ccgtgatgct cttcgcgaaa gtggacaagg agctgctgaa
     3961 aatgcagacc gaaaagctcg aaaagatcaa gaaacagcag gaagaagaaa attcgcggat
     4021 gggaaattcc caaccggcga gcctggaacc gcagaaagcc acgaccacgt tcgacgaatt
     4081 ttcgaaaatg gacatccgca ccggcacgat catcgcagcg gaaaaaatgc cgaagagcga
     4141 caagctgctg aagctcgtcg tcgacaccgg tctcgaccag cgcacggtgc tgagcggcat
     4201 cgcgaaatat tattcgccgg agaatatcat cgggcagcag gtgacgatcc tcgtgaacct
     4261 ggcaccgcgg aagatgatgg gcgtggaatc gaacggcatg atcctgatgg cggaaaacga
     4321 aaagggcgag cttagcttcg ttgcgccgac ggagaagaac atcaacaacg gatcgacggt
     4381 gaaataagcc ctatgcttcg aattcttaat tttgaattcc ggattgcgtt catcatcatt
     4441 cagcattcac gtggtgccgc acttcggttg acgcggtgga tggaatagaa gtttttttgg
     4501 tcccgtagtt caatggatag aatagaagtt tcctaaactt ttgatacagg ttcgattcct
     4561 gtcgggacta caaaatggaa atgaagccgg ttatgcgaaa acgtgaccgg tttttattta
     4621 tccgacaaca aacgtttatt acctattact ttgcggcttt attcatatgg ttgtatgcta
     4681 tttcatgtga gtttttatga caaaattctt aggtatcgaa ggtttgaata aggttaagag
     4741 tctttttaat gcaatagccg ttatcgttgc acccttttcc atcgctacag cactcgtttt
     4801 gtggatcgaa aataaaaata atgcagctat cgctgtttta tggtggcaca atagttattt
     4861 tatttggcta ttgcttgcta ctattttttt aatgctttca actattttat atctcagttt
     4921 atcttcagac aaatcgaata ctattagatt gggggctaaa acagagaaga agaaaaccgg
     4981 atcaatgaaa aatatactgc gggttgcagt gattttaggt gtcgtcaatt tctttttggc
     5041 tctatttttt tatggaacga ctaaatttaa ttccgagcaa acaacgggca ttttaggaat
     5101 aacttattct aattcagaat ttcaagctgc actccaatct tttttgcggg gaaaatgcgg
     5161 ggaaaatgaa ctgcagttca cggataatca acgggctatt gaagacataa aatatcagga
     5221 tgctttattt gcaacatact ttcaagaagg actattgtac tatggtcatt gtacaaaagt
     5281 tgattcttta ttgctaattg attgtacact aaaatggcat ggacagaagc atactgatat
     5341 tgatacaaat caaactattc cttcctcagt gtatagaaat ctggatgact acagcttaaa
     5401 aataagaaca aatgcaaaaa ttatttcgga ttttattctt ggctattatt atgttcatta
     5461 ttccgatagt tctaaaaccg cgataaagta tttccaaaag atcattaatg attccatatt
     5521 tcaggcggct atcagaagca gaccgaaaga tgaagatttg caggcattta ataaagatct
     5581 tcaaatgttt caaaaatatg cagaactact tgttactgat cagtcgaaac aatctgcagt
     5641 tgttccctgc aataacggag aactgtggca gtatagcctg ggagggaaat tatcttctta
     5701 ccaaggtctt gttaagtcta cccaagaatt ctccgtcgat ttttactttg ataattacaa
     5761 tacagagtat aatagctact ctggagccac actagtggcc gcgatcaact cgatgtctaa
     5821 tcctgcattc attatttcag caataacttt acaaacagcg gttttgactc ctgaagaaag
     5881 ggattcgctg agtgaacgcc aacgagagag tttggaacaa aatagaacag catcggtgcg
     5941 ggactttatc tggagagaac ttctgcctat aaaaaaagaa atagatatag gcgactgcca
     6001 agtaagtcat gacattgcac caactatgcc ttggcccgtt tttcaagaag cgatctccaa
     6061 aaccaatgtt gaagacaaag atgtaattct ttcggtatta tctaaatatc cggatggcga
     6121 aaagagagaa gccgagataa ggaacatggc tgctacatat agggggttac cacagatttt
     6181 atacacacag gcgcaaattc atgttgtaat taaaggcgta gaattatggc catcctcaac
     6241 cattgttcgg aatgctttgc agaa
//
LOCUS       DASTSF010000119        12648 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_146557,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000119 DASTSF010000000
VERSION     DASTSF010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 12648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12648
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146557"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..359
                     /gene="uppS"
                     /locus_tag="VFU15_03510"
     CDS             <1..359
                     /gene="uppS"
                     /locus_tag="VFU15_03510"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012781151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HEU4716869.1"
                     /translation="AVKKIARDVKSGKTDVEKIDAELFSSYLTTAGYPDPELLIRTSG
                     EYRVSNFLLWQIAYAEFYFTEKLWPDFDREEFYKALVDYQSRERRFGKTSAQVDAGAK
                     NTGSLNGGAEKISSAR"
     gene            370..2847
                     /gene="bamA"
                     /locus_tag="VFU15_03515"
     CDS             370..2847
                     /gene="bamA"
                     /locus_tag="VFU15_03515"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HEU4716870.1"
                     /translation="MKNSFLTFIFLFILSFVHAQTDVNVVDYESPKEYTLVNVWVHGC
                     MTVDSAVVKLISGLPIGDKITVPGEKTADAIDNLWNEGMFEDVRIEVFRIEGDYIWLD
                     IIVTEKPKILGAQILGIKKKDREDLANKLHIRGTMLTDFRERYIIETCNEHFRQDGYL
                     NCKTTVVAVPIKDRPNYDSVYVHVDKGEKVKIQDIVITGNKKYKAGQIRWVMKDTKQK
                     RWYNIFKSSKYLEDNYEKDKDKVLAKYRKKGYRDARIVSDSVITVSPNRVMIKMNIDE
                     GQVYYFGNITWVGNTKYSSTILNDRLGIKPGDVYNQELLESRLYMSQNGNDISSLYMD
                     DGYLFFQVNPVETNVHGDSIDFEMRIYEGRQATIRNVTVVGNTKTNDKVILREIRTRP
                     GQLFRRSDVIRSQRELASLGYFDPEKIQINPKPDPYTGTVDIEYKVEERPNDQVELSG
                     GWGAGRIVGTVGVSFNNFSTHNFWKKDAWRPLPSGDGQKLSIRAQSNGYYYQSYNISF
                     TEPWLGGKKPNSLSITAYSSTQSNGLKFNDPLRTWVTISGVSVGYGIRLKRPDDFFNF
                     YMEANYQYYSLHNFYSVFAFSEGYSNNINLRFTLSRNSTDQLIYPRSGSDIKLIGEFT
                     PPYSYTIFRNRDYSDISTQDRYKFVEFYKWKFTSSWFTRIVGDLVLNTRIGFGYLGMY
                     NKAVGVSPFERFYLGGSGLTGYALDGREIIALRGYDDQSLTRSSTGSPIIAKYTWELR
                     YPVSLNPSATVFGLAFVEAGNTWNDIKDFNPFDVYRSAGVGVRVFLPMFGLLGLDWGY
                     RFDDVPTAPFMQHSQVHFTIGMNLGEL"
     gene            2931..3524
                     /locus_tag="VFU15_03520"
     CDS             2931..3524
                     /locus_tag="VFU15_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014678921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HEU4716871.1"
                     /translation="MKRKFIALVIALIASTGFSYAQKFCFVDTDYILNQSPDYQKAQQ
                     TLNDLSVQWQKEVEEKYAEIDALYKNYQQDQLLLTDDLRQKREAEIVQKEKDAKDFQK
                     QKFGVDGELFKKRQELIKPIQDQVYNAVKQMAEKGGYAIVFDKAGDMTMLYTNPKYDK
                     SDEVIDILGWKKAGRSSDNNNNNNSGGNTGGSGGDKK"
     gene            3566..4162
                     /locus_tag="VFU15_03525"
     CDS             3566..4162
                     /locus_tag="VFU15_03525"
                     /inference="COORDINATES: protein motif:HMM:NF015871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HEU4716872.1"
                     /translation="MKKLITAVIAFLLFTAPEFLHAQVKHPASAVAHLDLDSLLDIMP
                     AMKKASDSAQAYYNTLEQTLYTMQDDLNRRLNEYDSLNKTWSPLVKALKEKEIRDAQE
                     NLQAFQQNAQNDYANFRAQLVAPIFDQITKAVKEVAIAQGYQYVLDSSKSTGVVLYAT
                     KDNDIFDLVRIKLGIPAPPPPKPAGTGGTPGGAPGAVH"
     gene            4222..5013
                     /gene="murI"
                     /locus_tag="VFU15_03530"
     CDS             4222..5013
                     /gene="murI"
                     /locus_tag="VFU15_03530"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003288576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEU4716873.1"
                     /translation="MNKDAPIGIFDSGLGGLSVLRRVREELPSENIIYYADSAHIPYG
                     EKSLEEIRKLSLGVTEFLLEKGCKMIVIACNTASAAALKFLREEFPHVIFVGMEPAVK
                     PAAEQTQSGIVGVIATAATFQGELFASVIDRFAQNVTVLQQPCPGLVRQIENGETDSP
                     QTEEMLRGWLEPMKEKGIDALVLGCTHYPFVEKTIAKILGPGVQIIDPAPAVARRIKF
                     LLGGEKLLNENPQKGRVAYFTSGDTEKFSGMLEMLLHEKGNVRKA"
     gene            5026..5532
                     /locus_tag="VFU15_03535"
     CDS             5026..5532
                     /locus_tag="VFU15_03535"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEU4716874.1"
                     /translation="MKMEIAFYLDGLKRNAESFRSMLNGVPAGWKSSPGKWSLLEIVC
                     HLRDEEKEDFRARLQSVLEDPSKAFSPIDPQGWVLSRRYGEQKFDEAAEAFFREREAS
                     LAWLASLKNPAWENTYLHPAIGPMSAKFILSNWLAHDYLHMRQIMKLKYDYLGFCSEE
                     NLGYAGEW"
     gene            complement(5541..6053)
                     /locus_tag="VFU15_03540"
     CDS             complement(5541..6053)
                     /locus_tag="VFU15_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013870033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HEU4716875.1"
                     /translation="MPLILPVEGKLPQLGADCFIAENATIVGDVICGDQCSFWFNAVI
                     RGDVNSIRMGNKVNVQDGAIIHCTYNKTIASIGNNVSIGHRAIVHGCIIHDNVLIGMG
                     AIVMDNAEVGSNTIIAAGAVVTEGMKVEPHSIYAGVPAKKIKDISPELMQGEIQRIAN
                     NYVMYSGWFK"
     gene            complement(6100..6414)
                     /locus_tag="VFU15_03545"
     CDS             complement(6100..6414)
                     /locus_tag="VFU15_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007659051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-associated domain-containing
                     protein"
                     /protein_id="HEU4716876.1"
                     /translation="MNAQTDTSAKVSGNSSIVKIKTSAECDMCKKRIEQEVSKMKGVK
                     KATLDLDTKILTVEYNPKKTSPDKIRKAISDIGYDADDVKANNRASHELQHCTPKQDS
                     AH"
     gene            6764..7117
                     /locus_tag="VFU15_03550"
     CDS             6764..7117
                     /locus_tag="VFU15_03550"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU4716877.1"
                     /translation="MHIGKKIRVARVVKGFTQEDLAEKIGKTRPLISQIESTGKVKVG
                     TLKQICKVLDMDPEDPELVSFFEENEIYMTDSVRRKQRVRDFAVMEREVKMLRELVES
                     QKALIDEMKRKLGRK"
     gene            7196..7549
                     /locus_tag="VFU15_03555"
     CDS             7196..7549
                     /locus_tag="VFU15_03555"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU4716878.1"
                     /translation="MHIGKKIRVARVVKGMTQEDLAEKIGKTRPLISQIESTGKVKVG
                     TLKQICKVLDMDPEDPDLTSFFEENEIYLSGASKQKQRAKDFAIMERELKMLRELVES
                     QKALIDELKKKLGRK"
     gene            complement(7574..9151)
                     /gene="nadB"
                     /locus_tag="VFU15_03560"
     CDS             complement(7574..9151)
                     /gene="nadB"
                     /locus_tag="VFU15_03560"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HEU4716879.1"
                     /translation="MQRQTDFLIIGSGIAGLTFALKVAAHGKVMMITKSNEDESNTKY
                     AQGGIAAVMYKPDSYEKHIKDTLICGDGLCDEEVVRMVITESTERVMELAEWGARFDK
                     TPEGDYELAREGGHSEHRILHHKDITGYEIERALLSQVHTHPDIEILDHHYAVEIITQ
                     HHLGVEVNSRTPGITCYGAYILDTKSGKVETVLSRITLMASGGAGHVYATTTNPVIAT
                     GDGIAMVYRAKGKVENMEFMQFHPTSLYHPGERPSFLITEAMRGHGAVLKTRLGKEFM
                     QKYDERKSLAPRDIVARAIDNEMKLSGDDFVYLDCTGIEAEDLKAHFPNIYKKCLSIG
                     IDITKDFIPVVPAAHYLCGGIRVDKKGHSSIEHLFAVGECSCTGLHGANRLASNSLLE
                     AVVYSHTSSQEAIARFREIPFMSGIPDWNTEGTDAPEEMVLITQSLKEVQAIMSSYVG
                     IVRSDLRLQRAFDRLEILWRENEALFKKTTVSQQVCELRNIINCAYIIIKHARARKES
                     RGLHYTIDYPRKAAAGR"
     gene            9626..10732
                     /locus_tag="VFU15_03565"
     CDS             9626..10732
                     /locus_tag="VFU15_03565"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017733919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HEU4716880.1"
                     /translation="MKTIRFTGTGKPQFAAAVRKNVNAYFREKNFSTKGGWRMTLKTL
                     AMLGLYVVPFVFVFLFTMNPWVFLLLSVVMGAGMAGTGMAVMHDAAHGSYSRKDWMNK
                     IFSGTIYLLGGNLFNWKIQHNVLHHTFTNIEGYDEDIRSRVVIRMSKHAPLNKFHRFQ
                     HLYVFFLYSLMTLMRLGGDFFQLHEYNRKGLTKANKAHPVREYVKLVVGKVMYLLVAL
                     GLPLLLVSQSWWMILLAFVVMHLTAGMIMSVVFQLAHVVEGAEQPVPDGQGSIANDWM
                     IHELMTTANFSRRNRLLSWYIGGLNFQIEHHLFPGISHVHYHRIAPIVERTAREFGLP
                     YNQKPTFFHAFISHVRMLKELGRLQPVPVRVMKK"
     gene            10826..11446
                     /locus_tag="VFU15_03570"
     CDS             10826..11446
                     /locus_tag="VFU15_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018341912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HEU4716881.1"
                     /translation="METKQIPTVIYAESTPNPASMKFVANRMLISEGATAEYLNAGET
                     AKAPLPAKLFRFPFVKSVFISANYVTITKTEIVTWDDITLELREFFRDYLSKDLPVIE
                     ELPSKEVHTDSSFKSTTTVFTEHSVPQNETEHKIVEVLEQYIRPAVEQDGGLITFRSF
                     ENGTVTVQLKGSCSGCPSSTVTLKAGIEALLKRMVPGVKEVVAEAV"
     gene            complement(11490..>12648)
                     /locus_tag="VFU15_03575"
     CDS             complement(11490..>12648)
                     /locus_tag="VFU15_03575"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type B sorting domain-containing protein"
                     /protein_id="HEU4716882.1"
                     /translation="PFTYDWQPLGGNAGTATGLCAGTYLCTVTDSTGCASTYTTTILS
                     PQPLIVSPAANVTICEGQSTTLGAAVSGGTPPYSYAWSNNLPNAPSNTITPLQTTSYT
                     LMVTDANGCASNEQQTLVKVNPSPVSDFSPTSGACPPAEIFFTNLTDSAVAYHWIFGD
                     PSSGTNDTSSLMNPSHVFNAGGNYTITLITTNVYGCTDTLVRTDGASLLQEPHAGVSS
                     GAQFITTLDPVSAFDNLSSGGTSFFIDFGDGDSLFTTSYGPYMHTYDSIGIYNVMLVA
                     WSADGCTDTAWVPLELQEATTLFIPNAFTPNGDGTNDIFFAYGINVSDFDLMIFDRWG
                     MLLYETNDMSKGWNGTFKGEKCQEDVYVWKLRYHDNLGNAYEKTGHVSLVR"
BASE COUNT         3044 a         3226 c         3511 g         2867 t
ORIGIN      
        1 aagcggtgaa aaagatcgcg cgtgacgtga aatcggggaa aacggacgtt gaaaagatcg
       61 atgcggaatt gttcagcagc tacctgacga cggccggcta tcccgacccg gagctgctta
      121 tccgcacgag cggagaatac cgtgtgagca atttcctgct gtggcagatc gcttacgcgg
      181 aattttattt caccgaaaag ctctggccgg atttcgaccg cgaggaattt tacaaagcgc
      241 tcgtcgatta ccagtcgcgc gaacgccgtt tcggaaaaac aagcgctcag gtggatgccg
      301 gcgctaaaaa tacaggatca ttaaacggag gtgcggaaaa gatcagctct gcgcgctgaa
      361 aaacgaatta tgaaaaacag ctttctcaca tttatctttc ttttcattct ctccttcgtg
      421 catgcgcaga cggacgtgaa cgttgtcgat tacgaaagcc cgaaggaata tacccttgtc
      481 aacgtgtggg tgcatggctg catgaccgtg gattccgccg tggtgaagct catttccggt
      541 cttcccatcg gcgacaagat cacggtgccc ggcgaaaaaa cagcggacgc catcgacaac
      601 ctgtggaacg agggcatgtt cgaggatgtc cgcattgaag tgttccgcat cgaaggcgat
      661 tacatctggc tcgacatcat cgtgacggaa aaaccgaaga tcctcggcgc acagatcctc
      721 ggcatcaaaa agaaagacag ggaagacctg gccaacaagc tgcacatccg cggaaccatg
      781 ctcacggatt tccgcgaacg ctacatcatc gaaacgtgca atgagcattt ccgccaggac
      841 ggttacctga attgcaagac taccgtggtc gctgttccca tcaaggacag gcccaactac
      901 gattccgttt acgttcacgt ggacaaaggc gaaaaagtga agatccagga tattgtgatc
      961 accggcaaca agaagtataa ggcagggcag attcgctggg tgatgaaaga caccaagcag
     1021 aaacggtggt ataatatttt caaatcgtcg aaatacctcg aggataatta tgaaaaggac
     1081 aaagacaagg tcctcgcgaa ataccgcaag aaaggttatc gcgatgcgcg catcgtgtcg
     1141 gattccgtga tcacggtttc accgaaccgc gtgatgatca aaatgaacat cgatgaaggg
     1201 caggtgtatt atttcgggaa tatcacgtgg gtgggaaata cgaagtattc ttccaccata
     1261 ctcaatgatc gcctcggcat aaaaccggga gacgtttaca accaggagct gctcgaatcg
     1321 cgcctgtaca tgagccagaa cgggaacgac atcagctcgc tgtacatgga cgacggttat
     1381 cttttcttcc aggtcaaccc ggtggaaaca aatgtgcacg gcgattccat cgattttgaa
     1441 atgcgcattt acgaaggccg ccaggccacg atccgcaacg tgacggttgt cgggaacacg
     1501 aagacgaacg acaaggtcat cctgcgcgag atacgcacac gcccgggaca gctcttccgc
     1561 cgttccgatg tgatccgctc acagcgcgaa ctggcttcac tcggctattt cgatcctgaa
     1621 aaaatacaga tcaacccgaa acccgatccg tacaccggga ccgtggatat cgaatacaaa
     1681 gtggaagagc gcccaaacga ccaggtcgag ctgtccggcg gatggggcgc cggtcgcatc
     1741 gtgggcacag tgggcgtttc gttcaataac ttctccacgc ataatttctg gaagaaagac
     1801 gcctggcgtc cgctgccttc cggcgacggg cagaagctca gcatccgtgc gcagtcgaac
     1861 gggtattatt accagtcgta caacatttcg ttcaccgaac cctggctcgg cggaaagaag
     1921 ccgaattcac tttccatcac cgcttattct tccacgcagt cgaatggact gaagttcaac
     1981 gatccgctgc gcacatgggt gacgatcagt ggcgtttccg tcggctacgg aattcggctc
     2041 aagcgcccgg acgatttctt caacttctac atggaggcga actaccagta ttattcgctg
     2101 cataattttt attccgtgtt cgctttttcc gaaggttatt ccaacaacat caaccttcgc
     2161 ttcacgctca gccgcaattc caccgaccag ctcatttacc cgcgcagcgg ttccgacatc
     2221 aagctcatcg gggaattcac gccgccgtat tcctatacca ttttccgcaa ccgcgattac
     2281 tcggatattt ccacgcagga tcgttacaag tttgtcgaat tctacaaatg gaaattcacg
     2341 tcgtcctggt tcacgcgcat cgtcggcgac ctcgtcctga acacgcgcat cggcttcggt
     2401 tatctcggca tgtataataa agcggtcggc gtttctcctt tcgaacgttt ctacctcggc
     2461 ggaagcggtc tcaccggcta tgcgctcgac ggacgcgaga tcatcgcgct gcggggttat
     2521 gacgaccagt cgctcacccg ttcgagcacg ggatcgccca tcattgccaa atacacgtgg
     2581 gagctgcgtt atcccgtatc gctcaacccg agcgcaacgg tgttcggact tgccttcgtg
     2641 gaagcgggca atacctggaa cgatatcaag gatttcaatc cgttcgacgt ttaccgttca
     2701 gccggtgtcg gcgtccgtgt gttcctgccg atgttcggcc tgctcggtct cgactggggc
     2761 tatcgtttcg acgatgttcc cacggctccg ttcatgcagc attcgcaggt gcatttcacc
     2821 attggtatga atcttggaga actgtaaaca ttattaattc gtactttagt cgccccaaaa
     2881 cggcgtattc attaacactg tttgccggaa acggcccaaa aacgaaaccc atgaaacgta
     2941 aattcatcgc gctcgttatc gctcttatcg cctccacggg attttcctat gcacagaagt
     3001 tctgcttcgt ggatacggat tatattctca atcaaagccc cgattaccag aaagcccagc
     3061 agacgctgaa cgatctttcc gtgcaatggc agaaggaagt ggaagagaag tacgcggaga
     3121 tcgacgcgct gtataagaat taccagcagg accagctgct cctcacggat gatcttcgcc
     3181 agaaacgcga agccgagatc gtgcagaagg aaaaagacgc taaagatttc cagaagcaga
     3241 aattcggtgt ggacggcgag cttttcaaaa agcgccagga gctgatcaag ccgatccagg
     3301 accaggtata caacgccgtc aagcaaatgg cggaaaaagg cggctacgcg atcgtattcg
     3361 acaaagctgg cgacatgacc atgctttata ccaatcccaa atacgataaa agcgacgaag
     3421 tgatcgatat cctcggctgg aaaaaggccg gcaggtcttc tgataacaac aataacaaca
     3481 atagcggcgg taataccggc ggatcgggag gcgataaaaa gtaagatttc ctaaacgaaa
     3541 accatcaaca gaccaaaaca acaatatgaa aaagctcatt acagctgtaa ttgcattcct
     3601 gctctttacc gcgccggaat ttcttcacgc gcaggtgaag cacccggcat ctgcagtggc
     3661 acaccttgat ctcgattcat tgctcgacat aatgcccgcc atgaaaaaag cgagcgattc
     3721 ggcgcaggct tattacaata cgcttgagca gacgctctat acgatgcagg acgacctcaa
     3781 ccgccgcctg aatgaatacg attcgctcaa caaaacatgg agcccgctcg tgaaagcgct
     3841 caaggaaaaa gagatccgcg atgcacagga aaacctccag gccttccagc agaatgcgca
     3901 gaacgattac gcgaatttcc gcgcgcagct cgtcgctcct attttcgacc agatcaccaa
     3961 ggcggtgaaa gaagtcgcta ttgcacaagg ttaccagtac gtgctcgaca gcagcaaatc
     4021 caccggcgtg gtgctgtatg caacgaaaga caatgatatt ttcgatcttg tccgaatcaa
     4081 gctgggcatt cctgctcctc ctccgcccaa gccggcagga acaggcggta cacccggcgg
     4141 agcgcccggc gccgtacact gatcttacag acccgtcgga acgacgggtt tttttatgct
     4201 tttttctgaa ctttgaaccg catgaacaaa gacgctccca ttggtatttt cgattccggt
     4261 ctcggcggac tgagcgtgct gcgccgggtg cgggaagagc tcccttccga gaacatcatt
     4321 tattacgccg acagcgcaca tattccgtac ggtgaaaaat cgctggagga aatccggaag
     4381 ctgagtctcg gcgtcaccga attcctgctg gaaaaaggct gcaagatgat cgtcatcgcg
     4441 tgcaacaccg cgagcgcggc cgcgctgaag ttcctccgcg aagaatttcc gcacgtcatt
     4501 tttgtgggaa tggaacctgc cgtgaagcct gccgccgagc agacacagtc gggcatcgtc
     4561 ggtgttatcg cgacagccgc aaccttccag ggcgagcttt ttgcttccgt catcgatcgt
     4621 tttgcgcaga acgtgaccgt gttgcagcag ccgtgtcccg gtcttgtgcg ccagatcgaa
     4681 aacggagaaa ccgattcgcc gcaaacagaa gaaatgctcc gcgggtggct ggaaccgatg
     4741 aaggaaaaag ggatcgatgc actcgtgctc ggctgcacgc attacccgtt cgtggaaaaa
     4801 acgattgcga agatcctcgg gcccggcgtt cagatcattg atcctgcgcc tgccgtggca
     4861 agacggatta aattcctgct cggcggggaa aagctgctga atgaaaatcc gcagaaggga
     4921 agggttgcgt atttcaccag cggcgatacg gaaaaattca gcggcatgct ggaaatgctg
     4981 ctgcacgaaa aaggaaatgt gcgaaaggca taaccgggcg acagcatgaa aatggaaatc
     5041 gcattttacc tggatggcct gaaacgcaac gcggaatcgt tccggtcgat gctgaacggt
     5101 gtcccggccg gctggaaaag cagccccgga aaatggtcgc tgctcgagat cgtctgtcac
     5161 ctgcgcgatg aggaaaaaga agatttccgt gcgcggctgc aatccgttct cgaagacccg
     5221 tcgaaggcat tttctcccat cgatccgcag ggctgggtac tatcgcgcag gtatggcgaa
     5281 cagaaattcg atgaagcggc ggaagcgttt ttccgtgaac gcgaggcatc gcttgcctgg
     5341 ctggcatcat tgaaaaaccc ggcgtgggag aatacctatc ttcacccggc gatcgggccc
     5401 atgtccgcga aattcatcct gtccaactgg ctcgcacatg attacctgca catgcggcag
     5461 atcatgaagc tgaagtatga ttacctggga ttttgttcgg aggaaaacct gggatacgcc
     5521 ggcgaatggt gatgcggctg ttatttgaac cagcccgaat acatcacgta gttattcgcg
     5581 atgcgctgga tctcgccctg catgagctcg ggtgaaatgt ctttgatctt ttttgccggg
     5641 acacccgcat agatgctgtg cggttccact ttcattcctt ccgtcaccac ggcgcctgct
     5701 gcgatgatcg tattgcttcc aacttccgca ttgtccataa cgatagcgcc cataccgata
     5761 agcacgttgt cgtggatgat gcagccgtgc acgatcgcgc ggtgcccgat ggaaacatta
     5821 ttgccgatgg aagcgatggt tttgttgtag gtgcagtgga tgatcgcgcc gtcctgcacg
     5881 ttcactttgt ttcccatgcg gatgctgttc acgtcgccgc gtatgacggc attgaaccag
     5941 aagctgcact ggtcgccgca aatgacatca ccgacaatgg tcgcgttttc tgcgataaag
     6001 cagtcggcgc cgagttgcgg aagctttcct tcaacgggta gaatgagagg catttttcag
     6061 gtgttcggtt gttggttacc ggttcgcagt acgcgctgct cagtgcgcag aatcctgctt
     6121 cggtgtgcaa tgctgcagtt cgtgcgaggc gcggttgttc gccttcacat cgtccgcgtc
     6181 gtaaccgatg tcggagatcg ctttgcggat cttgtcggga gaagtttttt tagggttgta
     6241 ttccacggtc aggatcttgg tgtcgaggtc gagcgtggct tttttaacgc ctttcatttt
     6301 gcttacttcc tgctcgatgc gttttttgca catatcgcat tcggcagagg tctttatttt
     6361 gacgatggac gagtttcccg aaacctttgc gctcgtgtca gtctgagcgt tcaagcccgc
     6421 gctgcagaaa aatacgagaa gcagcgacag gagggcgaaa gtgtaatgct ttttcatggc
     6481 tggtgaatag gagttgaagt taatgagaag aatcagaaaa ctgaattcgg atttttgtcg
     6541 cgtgaaaaat cattgtttgc atatttgcaa tccttgcaaa atccgggccg gaataaaaaa
     6601 taaaccggcg accattgggg gataatgatg cgccggaagg gaacttgaag ggtcccgctt
     6661 ttgatctggt atattgggaa gaggacgatc ggcccgaatt caaacttaca taatgtaagt
     6721 ttaattagca aatcgtaagt aaaaggcttt ccaatattgc atcatgcata tcggaaagaa
     6781 gatcagggta gcaagggtgg tgaaaggctt cacgcaggag gatcttgcag agaagatcgg
     6841 caagacgcgc cccctgattt cccagataga atctacaggt aaggtgaagg tgggcacgct
     6901 gaagcagata tgtaaagtgc tcgatatgga cccggaagat cccgagctgg tttccttctt
     6961 cgaagagaat gaaatttaca tgactgattc cgtacggcgg aaacaaagag tgagagattt
     7021 tgcggtaatg gaacgtgaag ttaaaatgct gcgtgagctg gtggaatcgc aaaaagcgct
     7081 tattgatgag atgaagcgga agctgggcag gaagtgattt ttgcgccctg gcattactta
     7141 cttattgtaa gtatttaata aaaaatgtaa gtgaaaggct ttccaatatt gcaatatgca
     7201 tatcggaaag aaaataaggg tggcgagagt ggtgaagggc atgacgcagg aagacctggc
     7261 ggaaaagatt ggaaaaacac gccccctgat ctcccagatc gaatcgaccg gaaaagtgaa
     7321 ggtgggtacg ctgaagcaga tatgcaaagt gctggatatg gacccggaag acccggacct
     7381 gacttcgttc ttcgaggaga acgagatcta cctcagcggc gccagcaagc agaaacaaag
     7441 agcgaaagat tttgcgataa tggagagaga acttaaaatg ctgcgtgaac tggtggaatc
     7501 gcagaaagcg cttatcgatg agctgaagaa gaagctcggc cgtaaataat tccccttttt
     7561 tattgtgtat acgttaccgg cctgcggcag ccttccgcgg gtaatcgatc gtgtaatgaa
     7621 gtccgcggct ctctttgcgc gcgcgcgcat gcttgatgat aatgtacgcg cagttgatga
     7681 tgttgcgcag ctcgcacacc tgctgtgaaa cggtggtttt cttgaacagc gcttcgttct
     7741 cgcgccacag gatttccagc cggtcgaacg cgcgctgcag gcgcaggtcg gaacgcacga
     7801 tgcccacgta gctgctcatg atggcctgca cttctttcag gctttgcgta atgagcacca
     7861 tttcttccgg cgcgtcggtt ccttccgtgt tccagtcggg gatgccgctc atgaacggga
     7921 tttcccggaa acgcgcgatc gcttcctggc tggatgtgtg ggaatagacc acggcttcca
     7981 gcagcgaatt cgaggcgaga cgattggcgc cgtgcagtcc cgtgcaggaa cattcgccca
     8041 cagcgaaaag atgctcgatg ctgctgtgtc cttttttgtc gacgcggatc ccgccgcata
     8101 aataatgcgc agcgggaacg acgggaatga aatctttcgt gatgtcgatg ccgatgctga
     8161 ggcatttttt gtagatgttc gggaaatgcg ctttcaggtc ttccgcctcg atgcccgtgc
     8221 aatcgaggta cacgaaatcg tcgccggata atttcatctc gttgtcgatg gcgcgtgcga
     8281 cgatatcgcg cggggcgagc gatttccgct cgtcgtattt ctgcatgaac tcttttccca
     8341 ggcgtgtttt cagtaccgca ccatgaccgc gcatagcttc ggtgatgaga aacgacgggc
     8401 gttcccccgg gtgatacagc gaagtaggat ggaactgcat gaattccatg ttctccactt
     8461 tgccttttgc acggtacacc atcgcaatgc cgtcgccggt ggcgatgacc ggattggtgg
     8521 tggtggcgta cacgtgacct gcgccgcccg aggccatgag cgtaatgcgg gaaagcacgg
     8581 tttccacttt cccgcttttc gtgtcgagga tgtaagcgcc gtagcaggtg atgcccggcg
     8641 tgcggctgtt cacttccacg ccaaggtgat gctgcgtgat gatctcgaca gcgtaatgat
     8701 gatcgaggat ctcgatgtcc gggtgcgtgt gcacctgcga aagcagcgcg cgctcgatct
     8761 cgtagccagt gatgtccttg tggtggagaa tgcggtgctc ggaatgcccg ccttcgcgcg
     8821 cgagctcgta atcgccttcc ggcgttttgt cgaaacgcgc gccccattct gcaagctcca
     8881 tgacgcgttc ggtggattcg gtgatgacca tgcgcacgac ttcttcgtcg cacaacccgt
     8941 cgccgcagat gagcgtgtct ttgatgtgct tctcgtacga atcgggtttg tacatcacgg
     9001 ctgcaattcc gccctgcgcg tacttcgtgt tcgattcgtc ctcgttggat ttggtgatca
     9061 tcatcacttt gccgtgcgca gccactttca gcgcgaacgt aagcccggcg atgccggaac
     9121 cgatgatgag gaagtcggtt tgcctttgca tgtgcatcaa aggtaaattt caatcggcga
     9181 tttgcaattg cagtcaaagc ggaaaccgcc gcgaagcgat ttttgcgtga gcgatagaag
     9241 cggaaagccc ggagctgccg ttgccggatt ttgacacgtg ttcagcctga cggggtgccg
     9301 atgcatgccc gtgcagcgag gacttgcagc ggatagcgcg gtgcgcctgt gcgggcacag
     9361 cgtgttcctg caggcgcaca cgcccgtaaa aaaacaggta gctgccagcg gtgaaaggtg
     9421 tgggtttatg ttaaatcggg gaagaataat ttttacacgg gcaccaaaca aagattcctg
     9481 tcgtatcttt aataacgtaa ggaataattc acgttgtgtt tcgtgtgaac gcgccccctg
     9541 ttttgctccc accctctttc agcgtttaag aatactgtcc gcgacggacg ccgctgatcc
     9601 tgcgaatatc actttaacga ttgcaatgaa aacgatccga tttaccggta cgggcaaacc
     9661 gcagtttgcg gcggccgtgc gaaagaatgt gaatgcttat ttccgcgaga agaatttctc
     9721 gaccaaaggc ggctggcgga tgacgctcaa gaccctggcg atgctgggac tttacgtggt
     9781 gccgtttgtt tttgtttttc ttttcacgat gaacccgtgg gtgttcctgc tgctttctgt
     9841 agtgatgggc gcgggcatgg ccggaacggg catggcggtg atgcacgacg cggcgcacgg
     9901 ttcctattcg cgaaaggact ggatgaataa aattttcagc gggacgatct accttctcgg
     9961 cggcaacctt ttcaactgga aaatacagca caacgtgctg caccatacat ttacgaacat
    10021 tgaaggttat gacgaagaca tccggtcgcg cgtggtgatc cgcatgtcga agcatgcgcc
    10081 gctgaacaaa ttccaccgtt tccagcacct gtatgtgttt ttcctctaca gcctgatgac
    10141 gctgatgcgc ctgggcgggg attttttcca gctgcatgaa tacaaccgga aagggctgac
    10201 gaaggcgaat aaagcgcacc cggtccgcga atacgtgaaa ctcgtggtgg gaaaagtaat
    10261 gtacctgctg gtggcgctgg gcctgccgct gctgctggtt tcccagagct ggtggatgat
    10321 cctgctggct ttcgtggtga tgcatcttac cgccggaatg atcatgagcg tggtgttcca
    10381 gctggcgcac gtggtggaag gcgccgaaca gcctgttccc gacgggcagg gcagcattgc
    10441 caatgactgg atgatccacg agctgatgac gaccgccaat ttttcgagac ggaaccgcct
    10501 gctcagctgg tacatcggcg ggctcaattt ccagatcgaa caccatttgt ttcccggcat
    10561 cagccatgta cattaccaca ggatcgcgcc catcgtggaa agaaccgcgc gggagttcgg
    10621 actgccgtac aaccagaagc ccacattttt ccacgcgttc atttcgcatg tacgcatgct
    10681 gaaggaactg ggaagactgc agcccgtgcc ggtgcgggta atgaaaaagt gaaacgccgc
    10741 agggcggttt tgttcccgga gcaaaacagc ggatcattgc agaatatgga acccggaaca
    10801 ttgaactttg aattaccttt gcagtatgga aacgaaacag attcccaccg tgatctacgc
    10861 cgagagcaca ccgaacccgg cgagcatgaa attcgtggcg aaccgcatgc tcatcagcga
    10921 aggcgccacg gccgaatacc tgaacgcagg tgaaacggcg aaggccccgc tgccggcgaa
    10981 gctgttccgt tttccattcg tgaagagcgt ttttatttcg gcgaattatg taacgatcac
    11041 caaaacggaa atcgttacat gggacgacat tacgctcgag ctgcgcgaat ttttccgcga
    11101 ttatctttcc aaagacctgc ccgtgatcga agagcttccg tcgaaggaag tgcatacgga
    11161 cagctcattc aaaagcacga ccaccgtttt caccgagcat tccgttccgc agaatgaaac
    11221 ggagcacaaa atcgttgaag tgctggagca atatattcgt cccgcggtgg agcaggacgg
    11281 cggactcatc acgttccgct cattcgagaa cggaacggtg accgtgcagc tgaaaggatc
    11341 gtgcagcggc tgcccgtcat ccacggttac gcttaaggcg ggaattgaag cgctgctgaa
    11401 acgaatggtt ccgggcgtga aagaagtggt ggcggaagcc gtttgaaatc aatcctggtt
    11461 ttataattcc cggagttgct ttgcgctgct cagcgtacga gcgaaacatg cccggtcttt
    11521 tcgtaagcgt tgccgaggtt gtcgtggtat cttaatttcc agacgtacac gtcttcctgg
    11581 catttttctc ctttgaacgt gccgttccat cctttgctca tgtcgttggt ctcgtacagc
    11641 agcatgcccc agcggtcgaa gatcatcagg tcgaaatccg aaacgttgat cccgtaagca
    11701 aagaaaatat cattcgtgcc gtcgccgttc ggcgtgaacg cgttcggaat gaacagcgtt
    11761 gtggcttcct gcagctccag cggaacccat gcggtgtcgg tgcacccgtc ggcgctccat
    11821 gcaacgagca tcacgttgta gatgccgatc gaatcgtacg tgtgcatgta cggaccgtag
    11881 ctggttgtga acagcgaatc gccgtcaccg aaatcaatga agaaggaagt tccgcccgag
    11941 ctgagattgt caaaggcgga aacaggatcg agcgtggtga tgaactgtgc gcctgagctg
    12001 acgccggcat gcggttcctg cagcagcgaa gctccatccg tccggacaag cgtgtcggtg
    12061 catccgtata cgttcgtggt aataagcgtg atcgtgtaat ttccgccggc attgaaaaca
    12121 tgggaaggat tcatcagcga tgatgtatcg ttcgtcccgg aagaagggtc accgaagatc
    12181 cagtggtatg cgacggcgct gtcggtgaga ttcgtgaaga aaatttcagc cggcggacaa
    12241 gctccgctcg tcgggctgaa atcggaaaca ggcgaaggat tgactttgac cagcgtctgc
    12301 tgttcattcg aagcgcaccc gttcgcatcc gttaccatca gcgtatagct ggtggtttgc
    12361 aacggcgtga tcgtattgga aggagcgttg ggaagattgt tgctccaggc ataggaatac
    12421 ggaggtgttc cgccggaaac ggcggcgcca agcgtggtgc tttgtccctc gcagatcgtg
    12481 acgttcgctg ccggcgaaac gataagcggc tgcggggaaa ggatggttgt cgtatacgtg
    12541 gaagcgcaac cggtggaatc ggtgacggtg cacagatacg ttcccgcgca aagcccggtg
    12601 gccgtaccgg catttccccc gagcggttgc cagtcatacg tgaagggc
//
LOCUS       DASTSF010000120         6603 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1724051,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000120 DASTSF010000000
VERSION     DASTSF010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 6603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6603
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1724051"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..667
                     /locus_tag="VFU15_03580"
     CDS             <1..667
                     /locus_tag="VFU15_03580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716883.1"
                     /translation="VTADKVKFKKTADGPVYTMDKSDIFMIVYENGRKEVFSNDSSTS
                     PSAATPTKGDDDDEIVPRKHYGGPRAGMTCFGPGTIAGKLHTNVISQFGWQFETRFFT
                     LDNGSSGLFEFVPMLGGLESGKFLPSATAIVGYRTGSGFEFGIGPNLSQSGFGIVFAG
                     GASFRSGKVLFPVNIAFQPSVNKTYYAGYYDASSGQYVPGERTLATGFRVSLLVGFNA
                     RKN"
     gene            697..1464
                     /locus_tag="VFU15_03585"
     CDS             697..1464
                     /locus_tag="VFU15_03585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716884.1"
                     /translation="MKALLLLFASFFCAMLFAQDTLVTSRGKFITGYIVAADSTTFSW
                     TTDTASQELNLIPRSSVIAIRHRNGTIEKFFSSDTIVTMEGDVIVCKVMEIGEDVVTY
                     IPWTDFAKDPVAKPASDVFMIRLHNGIREIISHSGKNSDSADYYTLGQNDAKMYYKTP
                     PGTIVAEVASGAATWILIGVVPGAIICFAKPQYLHTVANPNDNLIDTNDAYRRGFEEA
                     AGKKKQRACVVAWGAGVVGLPVVLIGVIIVDLLNNFG"
     gene            1558..2418
                     /locus_tag="VFU15_03590"
     CDS             1558..2418
                     /locus_tag="VFU15_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020213918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HEU4716885.1"
                     /translation="MKKIFTLSALALLCFGFSGEKKAYQVFDSKGKAATYEDILNAAK
                     GADIVMFGELHDNPIIHWLELELTKDLYDAKKDKLIQGAEMFEADDQLILNEYLAGKL
                     SENTFKDEAKLWPNYGTDYKPLVDFAKDHKVPFIATNIPRRYANLVYKRGLGSLDSLD
                     AEAKKFIAPLPIAYDSTVKCYADIFASAGGHGGQNLPKSQAVKDATMAYFMLQNWSPG
                     KTFLHYNGSYHSDNHEGIVWYLLRSNPRLKIVVISSTTQDNIDTLDKDSEGKGDFIIA
                     VPEDMTRTQQ"
     gene            2494..4098
                     /locus_tag="VFU15_03595"
     CDS             2494..4098
                     /locus_tag="VFU15_03595"
                     /inference="COORDINATES: protein motif:HMM:NF017050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HEU4716886.1"
                     /translation="MEIHDFIVVGSGCTGVMAAQTLAERGEKVLVIDGGITDDHYASL
                     LPEKNFSDLRRTDDSQERYFLGDNFESFPENELGTGAQLTPARQFLIRETEKYLPLVS
                     ENFSPVESLAEGGLGGGWGLGCCVFSPNELKKAGLPESEMAEAYQVVSDRIGISAAND
                     DARKFTAAQLRDVQEAPPAGPSALSLLEKYASKKNRLNRNGFFLGRPALALLTKEKDG
                     RQPLAFRDMDFYEDAGNAAWRPAVAIRQLRKKSNVRFESGWMATHFSETDDCVSVHAM
                     KIGTDEEKIFRCRRLILSPGVLGTARIVLRSMPGGGEKKLPLLCNPYTYVPCLVPSRL
                     GKKMPEKDFGFAQLSLFHDADGTESDIAMASLYTYRSLLLFRLIRETPLNYRDGRIFM
                     QHIAPALLIAGIHHPEIFRDTQHLFLEKSTSTLTGDQLHVNYRRGDADEEVKRREKKY
                     VSALRSMGAWALKTVRPETGASIHYAGTLPFSAEEKAFTLHPDGKLHGTKNVYVADGS
                     GFTFLPAKGLTLSLMANAHRVARGVK"
     gene            4111..4974
                     /locus_tag="VFU15_03600"
     CDS             4111..4974
                     /locus_tag="VFU15_03600"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU4716887.1"
                     /translation="MPGTLLITGSNGFIGSHLLRMFLEQGWKVTALVHRVPEKKIPGV
                     IYHESDLAAATIPPVAGTADAFIHCAYVKQEKNTDAFSANVNGTKKLLEFAGALHIPV
                     KIFISSLSATEHALSGYGRQKYELEKLFLADGGTVIRAGLVLGDGGLFGSMKNYLQRR
                     SIVPLFGDGRQPVQTVFIDDLANVVVKVANEKITGRYVIAEDRPVPYREFYAELCASL
                     GVKPKFIRCSYGLAAFLVTLAGFAGMQLPVTKDNLSGLKQMARVPSAADLEKLGVNIR
                     TWKESLAILKK"
     gene            complement(5021..5710)
                     /locus_tag="VFU15_03605"
     CDS             complement(5021..5710)
                     /locus_tag="VFU15_03605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEU4716888.1"
                     /translation="MAKTIRLRSLLAKYFNVSNSEAERMIGEGRVRVDGKAEGPSFTL
                     EEWHDVSVDGKSIRPAKSFVYFLFFKPRGTECTMSASVENNLRAFFPGNEDVFPVGRL
                     DKDSEGLLLVTNDGRLFSAIAGEDSGKEKEYVVSVDKEVNADFIRSMSQGVVIMGKKT
                     KPAKVFAAADPFSFRIILTQGLNRQIRRMCYKLGFNVTRLVRTRIDVFTLGEMKPGEI
                     KVIGAEKLESN"
     gene            complement(5717..>6603)
                     /locus_tag="VFU15_03610"
     CDS             complement(5717..>6603)
                     /locus_tag="VFU15_03610"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010602144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HEU4716889.1"
                     /translation="FNRIFHVRRSNSTSVARKNIREHYDLSNDFFALWLDPTMTYSSA
                     LFENENMPLQEAQQAKYRRLSEMMKLKPEHHVLEIGSGWGGNAIFMAKNYGCRVTSIT
                     ISEEQQKLAMERVEAAGLSDRVKIVLEDYRQMEGQFDRIVSVEMLEAVGAQFYDVYFR
                     QVQRLLKKDGVVALQVITCPDSRFEELRRGVDWIQKHIFPGSLLPSVAALNRAVNNTG
                     DLTLVDLKDMGQHYARTLSEWRGNFNRVKEKVIALGFDERFICKWNYYLCYCEAAFAM
                     RNINVMQMIYTRPNNLTW"
BASE COUNT         1596 a         1861 c         1685 g         1461 t
ORIGIN      
        1 ggtcactgcc gataaagtga aattcaaaaa gaccgcggac ggtcctgttt acacgatgga
       61 caaatcggac atcttcatga tcgtttacga gaacggacgg aaagaagtgt tcagcaacga
      121 cagcagcact tcaccttcag cggcaactcc gacaaaagga gacgacgacg atgagatcgt
      181 cccgcggaaa cattacggtg gtccgcgcgc aggcatgacc tgcttcggtc cgggaacgat
      241 agccggcaag ctgcacacga acgtgatctc ccagttcggc tggcaattcg aaacgcgttt
      301 cttcacgctc gacaacggca gttccggcct gttcgaattc gtgcccatgc tcggcggact
      361 cgaaagcgga aaatttcttc ccagcgccac tgccatcgtc ggttaccgca cggggtccgg
      421 ttttgaattc gggatagggc cgaacctttc gcagagcggg ttcgggatcg tgttcgccgg
      481 cggcgcttct ttccgttcgg gaaaagtttt gttcccggtc aacatcgcgt tccagccttc
      541 ggtgaacaaa acgtactacg cgggctatta cgacgcttcc agcgggcaat atgttcccgg
      601 tgaaagaaca ctcgccaccg gtttccgcgt gagccttctt gtcggtttca acgcacgaaa
      661 aaactaacca ggcacttcac taaaaaaatc agccacatga aagctctgct cctccttttc
      721 gcttcctttt tctgcgcaat gcttttcgca caggatacgc ttgttacgtc caggggaaaa
      781 ttcatcaccg gttatatcgt cgctgccgat tccacgacct tttcgtggac gaccgacact
      841 gcttcacagg aactgaatct cattccccgc tcctcggtca tcgccatcag gcatcgcaac
      901 gggacgatcg aaaaattctt ttccagcgac acgatcgtga ccatggaagg agacgttatc
      961 gtctgtaaag tgatggagat cggcgaagac gtggtgacct acattccctg gacggatttt
     1021 gcaaaagatc ccgtagccaa accggcttcg gacgtgttca tgatccgcct gcacaacggc
     1081 ataagggaaa taatcagcca tagcgggaag aacagcgaca gcgccgatta ttacacgctc
     1141 ggtcagaacg acgcgaagat gtattacaaa acacccccgg gaacgatcgt tgcggaagtt
     1201 gcttcgggcg cagccacatg gatattgatc ggcgttgtac cgggggccat tatctgtttt
     1261 gcaaaaccgc agtacctgca caccgtcgcc aacccgaacg acaatctcat cgacaccaac
     1321 gacgcttacc gtcgcggttt cgaagaagca gccgggaaga aaaaacagag agcctgcgtg
     1381 gtggcctggg gagccggcgt tgtcggtctg ccggtggtgc ttattggtgt gataattgtc
     1441 gaccttctga acaactttgg ctgataaaaa cctgcaaata tcttaaccgc ggtgatcatt
     1501 tttgcagcag cagcaaggga tcggaagaaa atgattttct ttatacaaag aaattccatg
     1561 aaaaagatat ttaccctttc tgcattggcg ctgctctgct tcggcttcag cggtgaaaaa
     1621 aaagcctacc aggttttcga ctccaaagga aaagctgcga cgtatgaaga catcctgaac
     1681 gccgccaaag gcgcggacat cgtgatgttc ggcgagctgc acgacaaccc gatcattcac
     1741 tggctggaac tggagctcac gaaagacctg tatgacgcga aaaaagacaa gctcatccag
     1801 ggtgcggaaa tgttcgaagc cgacgaccag ctcatcctca atgaatacct tgccggcaag
     1861 ctttccgaga acacgttcaa agacgaggcg aaactgtggc ccaattacgg caccgattac
     1921 aagccgctcg tcgactttgc gaaagaccat aaggttcctt tcatcgccac caatattccg
     1981 cgccgctatg cgaacctcgt gtacaaacgc gggctcggat cgctcgacag ccttgatgcg
     2041 gaagccaaaa aattcatcgc gccccttccg atcgcgtacg acagcacggt aaaatgttac
     2101 gccgacattt tcgcttctgc aggcggtcac ggcggacaaa atcttccgaa atcacaggcg
     2161 gtcaaggatg cgacgatggc ttatttcatg ctgcagaact ggtcgcccgg caaaacgttc
     2221 ctgcattaca acggcagcta ccattccgac aaccacgaag gcatcgtgtg gtacctgctc
     2281 cgcagcaatc ccaggctgaa gatcgtcgtg atctcgtcca ccacgcagga caacatcgac
     2341 acactcgaca aagactccga aggaaaaggt gatttcatca ttgccgttcc ggaggacatg
     2401 acaagaacgc agcaataaaa tttccaaaac aaattccagt tcccaaatcc ggtcaacggg
     2461 tttgggattt gttttttttg aacctttgcg tgaatggaaa ttcatgattt catcgtggtc
     2521 ggttcgggat gcaccggcgt gatggcggcg cagacgctgg cggaacgcgg tgaaaaagtt
     2581 ctcgtgatcg acggcgggat cacggacgac cattacgctt cgctcctgcc ggaaaaaaat
     2641 ttctccgacc tgcgccggac cgacgattcc caggaacgtt atttcctcgg cgacaatttc
     2701 gaatcctttc ctgaaaacga gcttggcacc ggcgcacagc tgacgccggc ccggcagttc
     2761 ctcatccgcg aaacggaaaa ataccttcca ttggtttcgg aaaatttcag cccggtggaa
     2821 agtcttgccg aaggcggact tggcggaggc tggggactcg gctgctgcgt tttttcaccg
     2881 aatgaactga aaaaagcagg actcccggaa agtgaaatgg cagaagctta ccaggttgtt
     2941 tccgatcgca tcgggatctc cgctgcaaat gacgacgcac ggaaattcac cgcggcacag
     3001 ctgcgtgatg tgcaggaagc accgccggcc ggtccgtccg cgctttccct cctggaaaaa
     3061 tatgcatcaa aaaaaaaccg tctcaaccgg aacggatttt ttctcgggcg ccccgcgctc
     3121 gctttgctga cgaaggaaaa ggacgggcgg caacccctcg ctttccgcga tatggatttc
     3181 tatgaggatg ccgggaatgc cgcgtggcgt ccggccgttg cgatccggca gctgagaaaa
     3241 aagagcaatg tccgtttcga aagcgggtgg atggcgacgc atttttccga aacggatgat
     3301 tgcgtttccg ttcacgcaat gaagatcgga acggatgaag aaaaaatatt ccgttgccgc
     3361 cgcctcatcc tttcgcccgg cgtgctggga actgcgcgca tcgtcctgcg ttcaatgccc
     3421 ggcggcggcg aaaaaaaact tccgctgctc tgcaatcctt acacgtatgt gccctgcctt
     3481 gttccgtcac ggcttggaaa aaagatgccg gaaaaggatt tcggcttcgc gcagctttcc
     3541 ctgttccatg atgccgacgg aacggaaagc gatatcgcga tggcatcgct gtacacctac
     3601 cgttcgctgc tgctgttccg tcttatccgg gaaacgccgc tcaactaccg cgacgggagg
     3661 atcttcatgc agcatattgc gccggcgctg ctgatcgcag gcatccatca tcccgaaatt
     3721 ttccgcgaca cgcaacatct tttcctggag aaaagcacat ccactcttac aggcgatcaa
     3781 ctgcatgtga actaccggcg cggtgatgcg gacgaagaag tgaaacgccg cgaaaagaaa
     3841 tatgtttctg cgctgcgctc gatgggcgca tgggcgctga aaaccgtacg gcctgaaacc
     3901 ggcgccagca ttcattatgc aggcacactt cccttttcag cggaagaaaa agcattcacg
     3961 ctgcacccgg atggaaaatt gcacggaaca aaaaacgttt atgtcgcaga cggttccggt
     4021 ttcacttttt tacccgcgaa aggattgacg ctttcgctta tggcaaatgc gcacagggtg
     4081 gcacggggcg tcaaataact aaattcgcac atgcccggca cacttctcat caccggcagc
     4141 aacgggttca tcggatctca cctgctgcgc atgttcctgg aacagggatg gaaggtcacc
     4201 gcgcttgttc accgcgtgcc ggagaaaaaa attcccggag tcatttacca cgaatccgac
     4261 ctggccgccg caaccattcc ccctgttgcc ggaacagcgg atgcattcat tcactgcgct
     4321 tacgtgaagc aggaaaaaaa cacggatgca ttttccgcga acgtgaacgg gacgaaaaag
     4381 ctcctggaat ttgcaggcgc gcttcacatc ccggtgaaaa ttttcatctc gagcctttcc
     4441 gccaccgaac atgcgctctc cgggtacggg cggcagaaat acgagctgga aaaattattt
     4501 ctcgcggatg gcggaacggt gatccgcgca ggacttgtgc tcggggacgg aggactgttc
     4561 ggctccatga aaaactatct tcagcgcaga agcatcgtgc cccttttcgg tgacggcagg
     4621 cagccggtgc aaaccgtttt catcgatgat cttgcgaacg tggtggtaaa agttgcgaac
     4681 gaaaagatca cggggcgcta tgtcatcgcg gaagaccggc ccgttcccta tcgcgaattt
     4741 tatgcggagc tctgcgcatc gctgggtgtg aaaccgaaat tcatccgctg cagttatgga
     4801 ctggcggctt tcctggtcac gctcgccgga tttgccggga tgcaattgcc ggtaacgaag
     4861 gacaatcttt cgggactgaa acaaatggcg cgggttccgt cggcagccga cctggaaaaa
     4921 ctcggggtga atattcgcac atggaaagaa agccttgcca tcctgaagaa gtgacgacca
     4981 cggcgcccgc ccaattgcct accggaaact tcccaacctt tcaattgctt tccaactttt
     5041 ccgccccgat cacttttatt tcccccggct tcatttcacc gagcgtaaaa acatcgatcc
     5101 gtgtgcggac aaggcgcgtc acgttaaagc caagcttgta acacatcctg cgtatctgcc
     5161 ggttgagtcc ctgcgtaagg atgatccgga atgagaaagg atccgccgcg gcaaatactt
     5221 tcgccggttt tgttttttta cccatgatca caacaccctg cgacatgctg cggataaaat
     5281 ccgcattcac ctccttgtcc accgacacaa catattcctt ttcctttccg ctgtcctcgc
     5341 cggcaatagc agaaaacaat cgcccgtcat tcgtcaccag caataatcct tcggagtctt
     5401 tgtccagtct tcccaccgga aaaacatctt cattccccgg aaaaaaagca cggagattat
     5461 tttccaccga agcgctcatc gtgcattccg ttccccgggg cttgaaaaaa agaaaataga
     5521 caaatgattt tgcaggacgt atgctcttcc cgtcgacaga aacatcgtgc cattcttcga
     5581 gcgtaaacga aggaccttcc gctttgccgt ccacgcgcac acggccttca ccgatcatcc
     5641 gttccgcttc gctgttgctt acattaaaat atttcgcgag aagggaacgc agccggatgg
     5701 tctttgccat tgaactttac caggtgagat tgttcggacg cgtgtagatc atctgcatca
     5761 cgttgatgtt ccgcatggcg aatgccgctt cgcaatagca caggtaatag ttccatttgc
     5821 agataaaccg ctcatcgaac ccgagcgcga tcactttttc tttcacgcgg ttgaaattcc
     5881 ctctccattc cgatagtgtg cgtgcataat gctgtcccat gtctttcagg tcgaccagcg
     5941 tcaggtcgcc ggtattgttc accgcgcggt tgagcgccgc caccgaggga agcagcgaac
     6001 cgggaaaaat gtgcttctgt atccagtcga cgccgcggcg caattcctcg aaacgtgaat
     6061 ccgggcacgt gatgacctgc agggccacga cgccgtcttt tttcagcagg cgctgcacct
     6121 ggcggaaata cacatcataa aactgcgcgc ccaccgcctc gagcatctcc acgctgacga
     6181 tacggtcgaa ctgtccttcc atctggcggt aatcttccag cacgatcttc acgcggtccg
     6241 aaagtccggc cgcttccacg cgttccatcg ccagcttttg ctgttcttcc gagatcgtga
     6301 tcgacgtgac gcggcatccg taatttttcg ccatgaagat cgcgttgccg ccccacccgc
     6361 ttcctatctc gagcacatga tgctccggct tcagcttcat catttccgaa agacggcggt
     6421 acttcgcctg ctgcgcttcc tgcagcggca tattttcatt ttcaaacagc gcgctggaat
     6481 acgtcatcgt gggatcgagc cagagcgcaa aaaaatcatt gctgaggtcg tagtgctcac
     6541 ggatattttt ccgcgccacc gatgttgaat tcgaccggcg cacgtggaag atccggttga
     6601 aca
//
LOCUS       DASTSF010000121         2184 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_146185,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000121 DASTSF010000000
VERSION     DASTSF010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2184
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146185"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..627)
                     /locus_tag="VFU15_03615"
     CDS             complement(<1..627)
                     /locus_tag="VFU15_03615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716890.1"
                     /translation="MKQFFITFSCFLLPAFLFSQELFPNTEPASNMPKGVLAFRIAGE
                     YYSEVGQPRIWTGYRFKYGFTARMEADLTIGLSNHHGDLLPPGFVSTDGNIGPHTHGG
                     KKGIYYPYRFEGIDIYAKYRFLNIDGEHRHFRMAAYAEISTARVAHDETEPNLMMEDN
                     AGAGGGIVATYLYHKLAVSTTLGGIFPVAFSGHQEMPNNERGPDITIHY"
     gene            complement(627..1136)
                     /locus_tag="VFU15_03620"
     CDS             complement(627..1136)
                     /locus_tag="VFU15_03620"
                     /inference="COORDINATES: protein motif:HMM:NF012621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HEU4716891.1"
                     /translation="MKKLLTAIFSVAFFHFSFGQFKKVVIRVDGLTCSACSYATQHSL
                     QTLSFVSDVQMDLNNNLATVTFREGRKVSIDSLAIKVVDAGFSVGSLSATMDLSGASV
                     TDSTCFVYQNDCYYVLAANGKKLGGTVDIRFVGEAYMKKSEYKPWKSKLNGSCGTSDM
                     KGKTYYVTL"
     gene            1238..1618
                     /locus_tag="VFU15_03625"
     CDS             1238..1618
                     /locus_tag="VFU15_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020213677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716892.1"
                     /translation="MRTVISASLLLLYTCAVLRPLYPFADYLLNRNYIANVLCENRDK
                     PKMHCNGKCHLMKELKKSVDDDPKAPAPSRTEVKYETSLYTIDESMALAISAHLTGLS
                     AWSDEETDHYASCAVSDIFRPPKA"
     gene            1715..>2184
                     /locus_tag="VFU15_03630"
     CDS             1715..>2184
                     /locus_tag="VFU15_03630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716893.1"
                     /translation="MNPRKAIALALCVIGLSAIRLCAQQPDSAAAPKFYDCCRPDGHA
                     PLGVMTTHIHAKGQWSFLYSYMYMDMRGNRKGTSKLSDDDIFATYAMAGDKMSMQMHM
                     LMIMYGISNRITVMGMLNYYQNNMSMSMLPMAMDMPGMTMASYNMPTSMKTSGLG"
BASE COUNT          528 a          582 c          556 g          518 t
ORIGIN      
        1 atagtgaatg gtgatatccg ggcccctttc gttattcggc atttcctggt gcccgctgaa
       61 cgccaccggg aaaataccgc cgagcgtcgt tgaaacggcg agcttgtggt agaggtacgt
      121 cgcaacgatg ccgccgccgg cgccggcatt atcttccatc atcaggttgg gctctgtttc
      181 gtcatgcgcc acgcgggccg ttgaaatctc cgcatacgcc gccatccgga aatggcgatg
      241 ctccccgtcg atgttcagaa aacggtattt ggcgtaaata tcgatccctt cgaaacggta
      301 agggtaataa attccctttt tgccgccgtg cgtgtgcgga ccaatatttc catcggttga
      361 aacaaaaccg ggaggaagaa gatcaccgtg gtgattggaa agcccgatcg tcaggtcggc
      421 ttccatccgc gccgtaaaac catatttgaa ccggtagccc gtccagatgc ggggctgccc
      481 tacttcgctg taatattcac ccgcaatgcg gaaagcaaga acgcctttcg gcatgttgct
      541 ggctggttcg gtattcggaa acaactcctg cgaaaacagg aacgcaggca gcaggaaaca
      601 agaaaacgta atgaagaact gtttcattac agcgttacgt aataggtttt gcctttcata
      661 tcgctggtgc cgcagcttcc gttcagcttc gatttccagg gtttatattc gctttttttc
      721 atgtatgctt cgcccacaaa acggatgtcg acagtcccgc cgagcttctt cccgttcgcc
      781 gcgagcacat aatagcaatc gttctggtaa acaaagcaag tcgaatcggt aacggaagcg
      841 ccggaaagat ccattgttgc ggaaaggctt cccacggaaa acccggcgtc cactactttg
      901 atcgccagcg aatcgatgct gacctttctc ccttcgcgga aggtcacggt cgccaggtta
      961 ttgttcaggt ccatctgcac atcgctgacg aagctcagcg tctgcagcga atgctgcgta
     1021 gcgtaggagc acgccgagca ggtgagtcca tcgacccgga taacgacttt tttgaactgg
     1081 ccgaaagaga agtggaaaaa cgctaccgag aaaattgccg tcagtaattt tttcattgga
     1141 accggtttgt gacggggcta ctcaaagata atcaatctca aaagcaatgc cacctcgtca
     1201 tttcatgtaa aaatgcgtaa atttgagtag cgtgaaaatg aggacagtca tatctgcgtc
     1261 gcttctcctg ctctatacct gcgcagttct gcgaccgctg tatcctttcg cggattacct
     1321 gctgaaccgg aattacatcg ccaatgtact gtgcgagaac cgggacaagc cgaagatgca
     1381 ttgcaacggg aaatgccacc tgatgaaaga gctgaagaaa tcggtggacg atgatcccaa
     1441 agcgcctgcc ccttcacgca ctgaagtgaa atatgaaact tccctttaca cgatagatga
     1501 atcgatggcg ctcgccatct ccgctcacct caccgggctc agcgcctggt ctgacgaaga
     1561 aacggaccac tacgcttcct gcgccgtttc ggatattttc cgtccgccga aagcctgatt
     1621 tttccatcct tttttgcagg ggcatgaaga cgatgcttcc cgctgttgtt tattctgata
     1681 agcagcgatt ttcttcattc taaaatttca ggtcatgaat ccacgtaaag caatcgcgct
     1741 tgcgttgtgt gtgatcgggc tgtccgccat ccggctttgc gcgcagcaac ccgattccgc
     1801 cgcagcgccg aaattctatg actgctgccg tcccgacggt catgcgccgc tcggggtaat
     1861 gacgacgcac attcatgcaa agggccagtg gtcgttcctt tacagctaca tgtacatgga
     1921 tatgcgcggc aaccggaaag gcacttcgaa gctcagcgac gatgatattt tcgccacgta
     1981 tgccatggca ggagataaaa tgtccatgca gatgcacatg ctgatgatca tgtacggtat
     2041 ttcgaaccgc atcacggtaa tgggcatgct caattattac cagaacaaca tgagcatgtc
     2101 gatgctgccg atggcgatgg atatgcccgg catgacgatg gcgtcgtaca acatgcccac
     2161 cagcatgaaa acgtccgggc tcgg
//
LOCUS       DASTSF010000122         5128 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_208883,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000122 DASTSF010000000
VERSION     DASTSF010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5128
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208883"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..634)
                     /locus_tag="VFU15_03635"
     CDS             complement(<1..634)
                     /locus_tag="VFU15_03635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716894.1"
                     /translation="MRTTKSQFYFFSIGGIKIPAVLIFIFFFAATAFAQPHVQSPADR
                     ARIDSIFRAIDAMPQDTATVSYINHVYNDYLWQFNLPEAYGRLKYAVRLSGKLNYDKG
                     EYVSYSNMAGFSMRLGNNYIELQNRLKALDAARRMNDSVRIVSQWFGIGVAYHGMGKD
                     SLALLYELDALRKFRQMVDTTTEQGKDRMSRTYLFIAWTYQSCGQYASALP"
     gene            complement(807..4235)
                     /locus_tag="VFU15_03640"
     CDS             complement(807..4235)
                     /locus_tag="VFU15_03640"
                     /inference="COORDINATES: protein motif:HMM:NF026220.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HEU4716895.1"
                     /translation="MKKIAAFLIAGLLACISAFSQQIAKPSPAVIAALPQWAQEMYSD
                     DPNVFRTDSLYNEYYSTHPFVQTSHTAYYRIWRHANIQYVNAEGYIRKPSAADQLQKR
                     NALRYNTSSSASAWTLVGPEKVFATDGSRTGEQTNIYSFTQCQSHPNYLYAGTEPGEI
                     YRSSDGGSNWQLCTLNDPLGGSVSAICVSPTDSSIVYAGSGDFIFKSSDGGNTWNAVF
                     ATGGLWANEILVHPADPNVVLVAANKGFFKTTDGGATWTTVYTQSCWDVKLRPGTGNV
                     VYLVKDNPSQLKAEFFLSSDTGSTFTIQSNGWYNSTDPARQDGGARIAVSPADPARVY
                     AYLIGQSKANDYGFIGVYRSNDGGATWTLPNGPDGGPYTSTHLNLAYGTPSWTYDQGF
                     YNCAIMADPNNADAILIGGLNLWKSTDGGASFTSVAGYIGGPLNIHVDQQDFRVGVGG
                     VWVTNDGGIYFSNDFFSSDNEVKMDGIHGSEFWGFGEGWNDDVMVGGLYHNGVVAYDE
                     DYGANNYLQLGGGEPASGYVNPGINKKVYSSEIGGAILPDAIGQPVQYLTLGMSPNES
                     YWTANSSEMEFDPRCYNIAWIGNANKLYRSTDGGGSFNLVYTFGTNTSANLSYFEISM
                     SNPDVMYVTQQPASGNQGKIWKTTDGGITFTQLTIPAGNSSRIIITLSPTSSDSLWIG
                     YPSNGNGSKIFRSGDGGATWTNYTTSDLDNQEVRSIQCIGATNGGVYLATDQTVYYRN
                     NTMGSWMPDNSGLPAELNSLYLRPFYRDSKIKISSYGHGVWENSLIDPPSHPVAQPQV
                     DKLNYTMNCVMDTFYFEDHSIINHAGASWQWSFPGGSPAASTLRNPKVVYPAPGTYTA
                     TLIVTDNTGQHDTGSVVITITPYAINNYLAEGFETSFPPNGWFLYDPDGDVQWSLANN
                     AGGYGQTAQSAVFDNYDFDSQGHDDDLRIRTDMTQQSTHWLKFDRAYSLYGAPYSDTL
                     EVLVSTDCGATFTSLYLKGGSQLATAPSLQSSTYVPAASEWATDSIDLSAWASSTDLM
                     IAFRNHGYFGQALYIDNINLSAMTGIHEQPSSAGNAMLVPNPVAAGQALVLQSDVAEK
                     FTVEIFDPEGRLILREQHMPGDHIALPANGTLPGVYFYRLTSDTMIRNSSFIVR"
     gene            4355..4591
                     /locus_tag="VFU15_03645"
     CDS             4355..4591
                     /locus_tag="VFU15_03645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716896.1"
                     /translation="MKNTTKLKHILQLYVVHLDRDDDENFVFALRDKRTGHTEVFADK
                     SWSVVIAKGFGHMKREMKFKEDRSPLSKKIKRKA"
     gene            complement(4705..4778)
                     /locus_tag="VFU15_03650"
     tRNA            complement(4705..4778)
                     /locus_tag="VFU15_03650"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4742..4744),aa:Met,seq:cat)
BASE COUNT         1218 a         1298 c         1410 g         1202 t
ORIGIN      
        1 acgggagcgc cgaagcgtat tgcccgcagc tctggtaagt ccatgcaatg aatagatacg
       61 tgcgggacat gcggtctttc ccctgctccg ttgttgtatc caccatctgc cggaatttgc
      121 ggagcgcatc cagctcatac aaaagcgcca gcgaatcttt tcccattccg tgatacgcta
      181 cgccgatgcc gaaccattgc gaaacgatcc tcacggaatc attcatgcgc cgcgctgcgt
      241 cgagcgcctt gagacggttc tgcagctcga tgtagttgtt gccgagccgc atcgaaaagc
      301 ccgccatgtt ggaataggaa acatattcgc ctttgtcgta attcagcttc ccggaaagcc
      361 tgacggcgta tttcagccgc ccgtatgctt ccggaagatt gaactgccag aggtaatcgt
      421 tgtaaacatg gttgatgtag ctgaccgtcg cggtgtcctg cggcatagcg tcaatggccc
      481 tgaatatcga gtcgatgcgg gcgcgatccg caggcgactg tacgtgaggc tgggcgaatg
      541 ccgttgctgc aaaaaagaaa atgaaaatca gtacggccgg aatttttatt ccgccaatgg
      601 aaaaaaaata aaactgcgat tttgtggttc gcattcggca ttcacatatt gccgcaaatt
      661 aggaaatacg gtggtaacgg aaacatgctt tcaaataccc gggatccgcg ggagcgggat
      721 cttgccaaac tggtttggtc ctgttcattt ggggaaacag gattgatttt ccgcataaaa
      781 ctggaaaaaa ttttcctccg ggtcttttac cttacgataa acgagctgtt ccggatcatc
      841 gtatcgctgg tcaggcgata gaaatagacg cccggcaatg ttccgttcgc gggcagcgca
      901 atatgatcgc ccggcatgtg ctgctcccgc aggatcagtc ttccttcggg atcgaaaatc
      961 tccaccgtga atttttccgc cacgtcgctt tgcagcacaa gcgcctgtcc tgccgcaacg
     1021 ggattgggaa cgagcatggc attccccgct gaagatggct gctcgtgaat tcctgtcatt
     1081 gcagaaaggt tgatgttgtc gatgtacagc gcctgaccga agtaaccgtg gttacggaaa
     1141 gcgatcatca ggtcggttga agaagcccaa gcagaaagat cgatgctgtc cgtcgcccat
     1201 tcggaagcgg cgggaacata agtgctgctc tgcagcgagg gcgcggtggc cagctgggaa
     1261 cctcccttta aatacagcga agtgaatgtt gctccgcaat cggtggagac cagcacttcg
     1321 agcgtgtcgg aatacggtgc gccgtaaagt gaataagccc ggtcgaattt cagccagtgt
     1381 gtgctttgct gcgtcatgtc ggtgcggatg cggagatcgt cgtcatgtcc ctggctgtcg
     1441 aaatcataat tgtcgaaaac ggcgctttgt gccgtttgtc cgtagccgcc ggcgttattc
     1501 gcgagcgacc actgcacgtc gccatccggg tcgtaaagga accagccgtt cggcgggaaa
     1561 gaagtttcga atccttcagc gaggtaattg ttgatcgcgt aaggcgtgat ggtgatgacc
     1621 acgcttcccg tatcgtgctg gccggtattg tccgtaacga tcagcgtggc cgtgtaggtt
     1681 cccggagccg gataaacgac tttcggattg cgcagcgtgg aagcagccgg acttccgccg
     1741 ggaaacgacc attgccagct ggcgccggcg tgattgatga tggaatgatc ttcgaaataa
     1801 aatgtatcca tcacgcaatt catcgtgtag ttaagcttgt cgacctgcgg ttgcgcgacc
     1861 gggtgcgaag gcggatcgat gagcgaattt tcccaaacgc cgtgcccgta gctcgagatc
     1921 tttatcttgc tgtcgcggta aaacgggcgc aggtaaagtg aattcagctc tgcgggcaaa
     1981 cctgaattat ccggcatcca gctgcccatc gtgttgttgc ggtaataaac ggtttgatcc
     2041 gtcgcaaggt aaacgccgcc gttcgttgcg ccgatgcact ggatggaacg cacttcctgg
     2101 ttgtcaagat cgctcgtggt gtaattggtc catgttgcgc cgccatcgcc cgatcggaag
     2161 attttcgaac cgtttccatt ggaaggataa ccgatccaca acgaatcgga gctcgtcggg
     2221 gaaagtgtga tgatgatgcg cgagctgttt cccgccggta tggtaagctg cgtgaacgtg
     2281 atcccgccat ccgtcgtttt ccagatcttt ccctggtttc cggaagcggg ctgctgtgtt
     2341 acgtacatca catccgggtt gctcatggaa atttcgaaat agctgagatt cgccgaagta
     2401 tttgttccga aagtgtaaac gagattgaat gatccgccgc cgtcggtcga cctgtaaagc
     2461 ttgttcgcgt tcccgatcca ggcgatgttg tagcagcgcg gatcgaattc catttcgctc
     2521 gaattcgctg tccagtaact ttcgttcggc gacattccca gcgtgaggta ctgcaccggc
     2581 tggccgatcg catcgggcag tatcgcgcct ccgatctccg aactgtacac tttcttgttt
     2641 atgcccgggt ttacgtaacc ggaagcaggc tctcccccgc ccagctggag ataattattc
     2701 gcaccgtaat cttcgtcgta cgccactacg ccgttgtgat ataatccgcc caccatcacg
     2761 tcgtcgttcc agccttcacc gaatccccag aattcagaac cgtgaatgcc atccattttc
     2821 acttcgttgt ccgaactgaa aaaatcattc gagaaataaa taccgccgtc gttcgtcacc
     2881 catactccgc cgacgcccac gcgaaaatcc tgctggtcaa cgtgaatgtt cagcggcccg
     2941 ccgatataac cggcaaccga tgtgaatgaa gcaccgccgt ccgtcgattt ccagagattc
     3001 agtccgccga tgagaatggc gtccgcgttg ttcggatcgg ccatgatcgc acagttgtaa
     3061 aatccctggt cgtaagtcca cgaaggtgtg ccgtaagcaa gattgaggtg cgtggatgtg
     3121 tacggtccgc cgtcaggacc attgggaagc gtccacgttg cgcctccgtc attgctgcgg
     3181 taaacgccga tgaaaccgta gtcattcgct ttcgactggc cgatgagata agcatacacg
     3241 cgtgccggat cagcggggga aactgcaatg cgcgcgccgc cgtcctgccg cgccggatcc
     3301 gttgaattgt accagccgtt cgactggatg gtgaacgtgg agccggtgtc ggacgaaaga
     3361 aaaaattcag ctttcagctg ggacgggttg tctttcacga ggtaaacgac atttcccgtg
     3421 cccggccgca acttcacgtc ccagcacgat tgcgtgtaaa cggttgtcca cgtggcgccg
     3481 ccgtctgttg ttttgaaaaa acctttgttg gctgccacca gcacgacatt cgggtcggcg
     3541 ggatgtacga gaatttcatt cgcccataat ccgccggtgg cgaatacagc gttccaggta
     3601 ttgccgccat cggaagattt gaaaataaaa tcaccgcttc cggcgtacac aatgctcgaa
     3661 tcggtgggcg aaacgcagat cgcggaaaca cttccgccca gcggatcgtt caaggtgcag
     3721 agctgccagt tcgaacctcc gtcgctgctg cggtaaattt cgccgggttc cgttcctgcg
     3781 tagagataat tgggatgcga ctggcattgc gtgaaactgt aaatatttgt ctgttcgccg
     3841 gtacgcgatc cgtcggtggc gaaaactttt tcagggccga cgagcgtcca tgcgctggcg
     3901 gaagaagagg tgttgtaacg caacgcgttc cgtttctgca gctggtcggc agcggacggt
     3961 ttgcggatgt agccttccgc atttacatat tggatattgg cgtggcgcca gatgcggtaa
     4021 taggccgtgt ggctggtctg tacgaacgga tgcgtggaat aatattcgtt gtaaaggctg
     4081 tcggttctga agacattggg atcatcgctg tacatttcct gcgcccattg cggcagggcg
     4141 gcgataacgg cgggtgaagg tttcgcgatc tgctgtgaaa atgcagaaat acaggcaagg
     4201 agtcccgcga tgagaaaagc agcaattttc ttcatggtgt ggaaacgatt tgaacaaagg
     4261 tgcaaaatcc ccctgcggaa aagtggtcgt attttgataa gggtgatgcg ttaattgacc
     4321 ggccggaaat catttgatta tctttaattc cccgatgaaa aacaccacga agctcaagca
     4381 catcctgcaa ttgtacgttg ttcacctcga cagggacgac gacgaaaatt ttgtatttgc
     4441 cctgcgggat aaacgaacag gccacacgga agttttcgcc gacaaatcct ggtcggtggt
     4501 gatcgcaaaa gggttcgggc acatgaaacg ggagatgaag tttaaggaag accgaagccc
     4561 gttatccaaa aaaataaaac ggaaagcgtg aaacaaactt ccgggaaacg gcttttcccg
     4621 gtaaggagag cggagaaaac ttttcgtgat gatttatccg gcaataatcc aaaaacaaaa
     4681 tcccccgggc tatccggggg attgtagcga gagagagact cgaactctcg acctcagcat
     4741 tatgagtgct gcgctctagc cagctgagct acctcgccat tttgccggat acactggctg
     4801 gaaccggctt aaaccgctca ttttcaaacg caagaagatc gtttcgcttt ttgcagtttt
     4861 tgaaaacggg acgcaaatat agcattttac atttccaaat attcaacatc ggcgcaggcg
     4921 tacgctgcaa aaaaaaaatt aagacttgcg atgtagtgat ttttttctac ttttcccccc
     4981 gataaaaagt taacagagta cgattttacc tgcaggaatg gcaaagaatt cgtcccggaa
     5041 aaaagacaag aaagcgaagg gaaaaaaggc gtctccgaag aaagcggcgc ctaaaaagag
     5101 cgcgtctaaa gcaaagcagc cggtaaag
//
LOCUS       DASTSF010000123         7261 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1037601,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000123 DASTSF010000000
VERSION     DASTSF010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 7261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7261
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1037601"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2305)
                     /locus_tag="VFU15_03655"
     CDS             complement(<1..2305)
                     /locus_tag="VFU15_03655"
                     /inference="COORDINATES: protein motif:HMM:NF024962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716897.1"
                     /translation="MNANYTRGWKILICLIIAFIQVNTEAYASHAQGGDLTYTCLGGN
                     QYRLRLAFYRDCSGTSAPGSATIDISSATCGQSLSVTLYPIAGTGQDVTPICPSMTTV
                     CNGGSNPGVQEYIYEGNVTLPQQCTDWVFSYTLCCRNAAINTIVNPGGDNFYIETHMN
                     NVAVPCNNSPTFSNRPVPFICDGQTYCFNHGAVDPDGDSLSYTLVTPATGPGTYVTYI
                     SPWSASQPLTSVPAVSFNPQTGDICMTPSMVLVTVMAVRVDEWRNGVNIGSVIRDIQI
                     HVINCTNNLPYITGINNTNQYSISSCAGSTINFNVYSYDADAGQSLTLTWNGAIPAAT
                     FTVSPGPTPVGTFSWTPGPNDISNTPYCFTISVTDDACPYAGSQTFAFCITVTGFGIN
                     ITSSGTNCGASNGSASVTVNGGTGPYNYQWLPSGGNNANANGLTAGNYTVNVTDASGC
                     VSTASVSVPQGPAPGNIQSSSVDVACYGGTTGTASVNVTGGQQPYTYTWSNGGNTSSI
                     NNLPAGTYSVQVTTANGCITTATITVTQPASALASNISAVTNITCFGGNNGSATENAS
                     GGTPPYVYSWNSSPSQSTPTASSLPFGIYSVAVTDANGCTSSNTVNITQPPALTSVPS
                     ATAVGCNGGTSGSAQVLISGGVPPYNTSWNTTPPQNSTSITNLPAGNYTATITDGNGC
                     VVTSTVAITQPAALSANITSSANELCNGGSTGTASASASGGTAPYSYSWNTSPSQSGP
                     NAGNLSAGSYVATVTDAHGCATITTVTI"
     gene            complement(2640..4736)
                     /locus_tag="VFU15_03660"
     CDS             complement(2640..4736)
                     /locus_tag="VFU15_03660"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU4716898.1"
                     /translation="MMRKHFHITLFFLFLLAGVSSLHAQKKKIIDSLQGQLAKEQPDT
                     QRVNILNLLASQYRFTDHAQHKYYADQAITLSKKTGYTKGMAGAYNMMGLACENEGRY
                     TAALAYYDSSLTAWKTVGDKSFEAKMLLNIANVYNEKADYKSAIDYTLRSQKAQQDLG
                     SVFGVAVCKLTLGNIYYGEGDSKAALTSYEEAWKMNRASDKNPELEAATLGNIAGMFN
                     DMGQYDSAMYYYRISLDYFLNGGFTSRLGSCYNNIGTVWEHLGHTDSAFYYHRKGLAL
                     HLQMSNTEGAASSLISLGDLFRKTGDPDSALVYYNNALVISKNIGTRDNELEVYYQLS
                     QVYKQKKDFEKALSYSERYIGLYDSIHGQEQTRAIEKLRNNYLLDQKDAEIKTAEALR
                     KLSDETNRRNFILFLSLSLIGLLLTGIVFQRYRTKQKHNRQLSLKNDEISTQKKEITD
                     SIDYAKKIQEAILPDHAGVRQLFPGSFVLWRPRDIVSGDFMWYRRSGERIYLACADCT
                     GHGVPGALVSIVGVNLLNETLDNSPSITTGNFLDRLHLRMVQSMNKDVTNRERKDGMD
                     ISLLCFDLSKNEVEFSGASRPFYFNDGTGLQQLRGDKFSIGGAKEIDGASSFATTQLP
                     LKKGSSFYLFSDGFADQFGESTNKKFMTKRFFDLISSMQNETMEQQCALIEKAFLEWK
                     GHQEQVDDVLVIGVRI"
     gene            complement(4733..6805)
                     /locus_tag="VFU15_03665"
     CDS             complement(4733..6805)
                     /locus_tag="VFU15_03665"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEU4716899.1"
                     /translation="MRRFFFFLLFLSSCTAFAQSEADSLVRLLPGISSDTARVNMLNR
                     IAKIYDDISDSTEFIYSRQALALAQKTGYTAGEARAHVLIGLGMQGRSNFTQAIQEEQ
                     LADTLYAQLGDTLGRAKALGNMANNEYYIGDYTSAADHSLRSLRFYEALGNHTGIFAN
                     RLTLGNVSFDQNDFSEALKQYKLALAECRYFQDTPNMAARALVSIGNVHNSLNEPDSA
                     AANYITADSIFEKIHAGYETSVCLNNLATIRQSQHRYAETRQLLIRCLALRRATPDSE
                     SVCSVYQNIGSLSRDLGKPDSALLCYYYSLKLAHFTGSRSQMLNCYEGLASTYADLRR
                     FDSAYYFLHRYKELNDSVAGEESVNAVNQLKQRYASEKQQHHIDLIESQRAADKANSE
                     RNIALLVIGIFVLLTLVTITLYRYRAKQKTNEALERQNMEITMQKKAITDSINYAKKI
                     QDSILPPLHVVKEILPDSFILYEPKDVVSGDFFWVEKRDGFAIFSAVDCTGHGVPGAL
                     MSVVGFNLLTQAVNEIGLTKPADILGHLDYGVNKLLRQSEEENTVKDGMDLALCSYHA
                     EKRLLQYAGVFNPAYVVSAGELIVLKADKFPIGINVDGVTDTYTHHEMTLKPGDMIYV
                     FSDGYADQFGGPLGKKFKYNQFRDLLLSIWQEPVDIQEKMLRDAFHRWRGNHEQVDDI
                     LVIGVRVK"
     gene            complement(6813..>7261)
                     /locus_tag="VFU15_03670"
     CDS             complement(6813..>7261)
                     /locus_tag="VFU15_03670"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEU4716900.1"
                     /translation="ALKQNTGGESRDGMDVSLCVFHNATGLIHYAGANRPLWVVSGET
                     LNEIRPDKIAIGGVQEETTRTFTHHEIQLRTGDCVYLSSDGYGDQFGGPKGKKFMVKQ
                     LQKLLLSIHGESMENQRRILDTNFSEWKGTHEQVDDVLVMGFRYIS"
BASE COUNT         1556 a         1819 c         2107 g         1779 t
ORIGIN      
        1 taatagtaac cgtggttatg gttgcgcagc cgtgcgcgtc cgtaactgtt gccacgtagc
       61 ttccggcgga taagttgccg gcgttcggtc ctgactgcga cgggctggta ttccaggaat
      121 aagagtaagg agctgttccc ccgcttgcgc tggcagaagc cgttccggtg gaaccaccgt
      181 tgcaaagttc atttgcagat gaggtgatgt tcgccgaaag cgcggcgggt tgcgttattg
      241 cgacagtcga agtgaccacg cagccgttgc cgtcggtgat cgttgccgtg tagttcccgg
      301 ccggcaggtt ggtaatgctc gtgctgttct gcggcggtgt ggtgttccag gatgtgttgt
      361 aaggagggac cccgcccgaa atcaggacct gtgcagaacc gctggtgccg ccgttgcatc
      421 cgaccgccgt tgcgctcggt acgctggtca gcgcgggcgg ttgcgtgatg ttcacggtat
      481 tcgaggaggt gcagccattc gcatcggtca cggcgacgga atagatcccg aacggaagcg
      541 atgatgcagt cggcgtgctc tgcgaaggcg aagaattcca gctgtagacg taaggcggtg
      601 tgccgcccga tgcgttctcc gtagcggaac cgttatttcc tccgaagcac gtgatattag
      661 taaccgcgct tatattcgaa gcaagcgcgc tggccggttg tgtgacggtg atcgtcgccg
      721 tggtgatgca cccgttggcg gtggtcacct gcacgctgta agttcctgcg ggaagattat
      781 taatggagga ggtgttgccg ccattcgacc acgtatacgt gtaaggctgc tgtcccccgg
      841 ttacattcac acttgcggtt cccgtcgtcc cgccgtagca ggcgacatcg acagaagagg
      901 actgaatgtt tccgggagca ggaccctgcg gtacactgac gctggcggtt gaaacgcatc
      961 cggaggcatc ggttacgtta actgtataat tgcccgctgt aagtccgttc gcgtttgcgt
     1021 tgttcccgcc ggaaggaagc cactggtaat tataggggcc tgttccgccg ttgacagtaa
     1081 cgctggccga tccgttcgat gcgccgcagt tggttccgct cgatgtgatg tttattccga
     1141 aaccggttac agtgatgcag aacgcaaatg tctgcgagcc cgcatacggg catgcatcgt
     1201 cggtgacgga gatcgtgaag cagtaaggtg tgttgctgat gtcgttcggt ccgggcgtcc
     1261 agctgaatgt gccgaccggt gtgggcccgg ggctgaccgt gaatgtcgct gcgggaatag
     1321 cgccgttcca ggtgagcgtg aggctctgcc cggcgtcggc gtcgtaggaa tagacgttga
     1381 agttgatcgt ggaaccggcg caggaagaaa tgctgtactg gttcgtgttg ttgatcccgg
     1441 tgatgtacgg aaggttgttc gtgcagttga tcacgtggat ctggatgtcg cggatcacgc
     1501 tgccgatatt caccccgttg cgccattcat caacgcgaac agccataacg gtgacgagca
     1561 ccatgctcgg tgtcatgcag atgtcgcccg tctgcgggtt gaaggaaacg gccggaaccg
     1621 aagtgagcgg ttgcgaagct gaccacggag aaatataggt aacatatgtg ccgggtcctg
     1681 tagcgggtgt tacgagtgtg taggaaagcg aatctccgtc gggatcgacg gcgccgtggt
     1741 tgaagcagta ggtttgtccg tcgcagataa aaggaaccgg gcggttcgag aaggtggggg
     1801 aattgttgca gggcactgca acgttattca tgtgcgtttc aatgtagaaa ttgtcgccgc
     1861 cgggatttac gatcgtgttg atcgcggcgt tacggcagca taatgtgtag ctgaaaaccc
     1921 aatctgtgca ctgctgtggt aaagtaacgt tgccttcata aatgtattcc tgtacgccgg
     1981 gatttgaacc tccgttacag acggtggtca tcgaggggca gatcggtgtg acgtcctggc
     2041 cggttccggc aattggataa agagtaactg aaagtgattg tccgcatgtg gctgatgaaa
     2101 tgtcgatggt ggcagatccg ggagctgaag tgccggagca gtcgcggtaa aaagccaggc
     2161 gtaatctgta ctggttaccg ccgagacagg tgtaggtgag gtcgcctccc tgtgcgtgcg
     2221 aagcatacgc ttctgtgtta acctggatga aagcgatgat caggcaaata aggatcttcc
     2281 atccgcgtgt gtagtttgca ttcataactc actctccttt ctggttttgg ttaaaaaaca
     2341 aagaactgct ttcttcaacg ttccgaacgg agcccaggtt tcacgcgggt tctgaacaat
     2401 ccgggcctca aaaaaatatt atgcatgaat aacaatttca acgcattgca aatcaatgac
     2461 aatgatcatt gcgcgaaaaa gtttcagaaa agacatgtaa cgaaaaaata ttccggacgt
     2521 ataattaatt tatatgccgt tccggaaaag tgaagaaacg ggggaggaaa gattcggctt
     2581 tgtttccggg atcctgttta cacagcagaa aataaaaaaa gaaaacgggg agatcttcgt
     2641 cagattctca caccgatcac cagcacatca tccacctgtt cctggtggcc tttccattcg
     2701 aggaatgctt tttcgatcag cgcacactgc tgttccatcg tttcgttctg catcgaggaa
     2761 atgagatcga agaaacgctt cgtcatgaat tttttattcg tcgactcgcc gaactggtcg
     2821 gcaaacccgt cggagaaaag ataaaaggaa gaaccttttt tcagcgggag ctgcgtcgtt
     2881 gcgaacgatg aggcgccgtc gatctctttt gctccgccga tggagaattt atctccgcgt
     2941 agttgctgca gaccggtccc gtcgttgaaa tagaacgggc gtgacgcgcc ggaaaactcc
     3001 acctcgtttt tcgaaagatc gaagcaaagc agcgagatgt ccatcccgtc tttccgttcg
     3061 cggttcgtaa cgtccttatt catcgattgc accatgcgca ggtgaagccg gtcgagaaaa
     3121 ttcccggtcg ttattgaagg tgaattgtcg agcgtttcgt tgagcaggtt caccccgaca
     3181 atggaaacga gcgcgccggg aacgccgtgt cccgtgcaat ccgcgcaggc gaggtaaatg
     3241 cgttcgcccg accggcggta ccacatgaaa tctccgctca cgatatcgcg cgggcgccac
     3301 agcacgaacg aaccggggaa aagctgccgc acgccggcgt gatccggcag gatggcctcc
     3361 tggatcttct tcgcataatc gatgctgtcg gtgatctctt ttttctgcgt ggagatttca
     3421 tcgttcttca gtgaaagctg gcggttgtgt ttttgttttg tgcggtagcg ctggaaaaca
     3481 attcccgtga gcagcagccc gatgagtgaa agcgaaagga aaagaatgaa attccgccgg
     3541 ttggtttcat cggaaagctt ccgcagcgct tcggcggttt ttatttccgc gtctttctga
     3601 tcgaggaggt aattgttgcg cagcttttcg atcgcgcgcg tctgctcctg cccgtggatg
     3661 ctgtcgtaaa ggccgatata gcgctccgaa tagctcagcg ccttttcgaa atcctttttc
     3721 tgcttgtaaa cctggctgag ctggtaatac acttcgagct cgttatcgcg tgttccgatg
     3781 ttcttcgaaa tgacgagcgc attgttgtag tataccagcg cggaatcggg atcaccggtt
     3841 ttgcggaaca ggtcgccgag ggaaatgagg gagctggcgg cgccttcggt gttgctcatc
     3901 tgcaaatgca gcgcgagacc tttgcggtga taatagaaag cggaatcggt gtgaccgaga
     3961 tgttcccata cggtgccgat gttgttgtaa caggacccga ggcgggacgt gaagcccccg
     4021 ttcaggaaat aatccaggga aatgcggtag taatacatgg cggaatcata ctggcccatg
     4081 tcgttgaaca tgcccgcgat atttcccagc gtcgcagctt ccagctcggg attcttgtcg
     4141 ctggcccggt tcattttcca ggcttcttcg taggaagtca gcgcggcttt ggaatctcct
     4201 tcgccgtaat aaatatttcc cagcgtcagc ttgcacaccg ctactccgaa aacgctgccg
     4261 aggtcctgct gcgctttctg cgaacggagc gtataatcga tcgccgattt gtaatccgct
     4321 ttttcgttgt aaacattcgc gatgttgagc agcattttcg cctcgaagct tttatccccg
     4381 acggttttcc aggcggtgag cgaagaatcg taataggcga gcgccgccgt gtaacgtcct
     4441 tcattctcgc aggcgagccc catcatgttg tacgcgccgg ccattccttt cgtataaccg
     4501 gtttttttcg aaagtgtgat cgcctggtcc gcgtaatatt tatgctgcgc gtgatccgtg
     4561 aagcggtatt gcgaggcgag aaggttcagg atatttacac gctgcgtgtc cggctgctct
     4621 ttcgccagct gtccctgcag gctgtcgatg atttttttct tctgtgcatg aagggaagaa
     4681 actcccgcca ggaggaacag gaaaaaaaga gtgatatgaa aatgtttccg catcatttca
     4741 cccggacgcc gatgacgaga atatcatcca cctgctcatg gtttccacgc cagcggtgaa
     4801 aagcatcacg cagcattttt tcctgtatgt ccacgggttc ctgccagatg gaaagcagga
     4861 ggtcgcgaaa ctggttgtat ttgaattttt ttcccagcgg accgccgaac tggtcggcat
     4921 agccgtcgga aaaaacgtag atcatgtcac ctggtttcag cgtcatttcg tgatgcgtgt
     4981 aggtgtctgt aacgccgtcg acgttgatgc cgatcgggaa tttgtcggct ttcagcacaa
     5041 tgagttcccc ggcggaaacg acatacgccg gattgaagac gcccgcgtat tgaagcagtc
     5101 ttttttccgc gtggtacgaa cagagcgcaa ggtccatccc gtccttcacc gtattctctt
     5161 cttccgactg gcggagcagc ttgttcaccc cgtaatcgag atgcccgaga atatctgccg
     5221 gttttgtcag gccgatctcg ttcaccgcct gtgtgagcag gttgaaaccc accaccgaca
     5281 tcagcgcacc cggaacaccg tgaccggtgc aatcgacagc agagaagatc gcgaagccgt
     5341 cgcgcttttc cacccagaaa aaatcgccgc tgacaacatc tttcggttcg taaagaataa
     5401 agctgtcggg aaggatctct ttcaccacgt gcaggggggg aagaatggaa tcctggatct
     5461 tcttcgcgta attgatgctg tcggtgatcg cttttttctg catcgtgatc tccatgttct
     5521 gccgctcgag cgcttcgttg gttttctgct tcgcgcggta gcggtagagt gtaatggtga
     5581 ccagcgtaag cagcacgaag atcccgataa cgagcagcgc aatgttgcgt tctgaattcg
     5641 ctttgtccgc tgcgcgctgc gattcgatca ggtcgatatg atgctgctgc ttttcggagg
     5701 cataccgctg cttgagctgg ttcacggcgt tcacgctttc ttcgcccgca acggaatcgt
     5761 tgagctcctt gtagcgatgg agaaaataat aggccgagtc gaatcgcctg agatcggcgt
     5821 acgtggaggc aagaccttcg tagcagttca gcatttgcga acgcgaaccg gtgaaatggg
     5881 ccagcttcag cgaatagtaa tagcagagca gcgcggaatc gggtttcccg agatcacgcg
     5941 aaagtgatcc gatgttctga tatacggaac agacggattc gctgtcgggc gtcgctcttc
     6001 tcagggcaag gcagcgtata agcagctgcc gcgtttccgc gtagcggtgc tggctttgcc
     6061 ggatcgtcgc gagattgttg aggcagaccg acgtttcata ccccgcgtgg atcttttcga
     6121 agatggaatc cgcggtaatg taattggcgg cggcgctgtc cggttcgttc agtgaattgt
     6181 gcacgttgcc gatgctgacc agcgcgcgtg cggccatgtt cggtgtatcc tggaaatacc
     6241 ggcattccgc gagggcaagc ttgtactgct tcagcgcttc gctgaaatca ttctggtcga
     6301 aagaaacatt tcccagtgtc aggcggttgg cgaaaatgcc tgtatgattt cccagcgctt
     6361 catagaagcg gagcgaacga agggaatgat cggcagctga tgtgtaatcg ccgatataat
     6421 attcgttgtt cgccatgttc cccagcgcct tcgcgcggcc gagcgtgtcg ccgagctgtg
     6481 catacaatgt gtcggcgagc tgctcttcct gtatggcttg cgtgaaattg cttcttccct
     6541 gcatgccgag cccgatgagt acatgcgccc gggcttcacc ggcggtatag cccgttttct
     6601 gcgccagcgc cagggcctgc cgggagtaaa taaattcggt gctgtcggaa atgtcgtcat
     6661 agatcttcgc aatacggttg agcatattca cacgcgccgt atcactggaa ataccgggca
     6721 gcaggcgtac caggctgtcg gcctccgact gcgcaaaagc agtgcaggaa gaaagaaaga
     6781 ggagaaaaaa gaaaaagcgg cgcatgaatt attcaggaaa tatagcgaaa gcccatgacg
     6841 agtacatcat ccacctgctc atgggtccct ttccattcgc tgaaattcgt atcgaggatc
     6901 ctgcgctggt tttccatgga ttctccgtgt atgctgagca aaagcttctg caattgcttc
     6961 accatgaatt ttttcccttt cggtccgccg aactggtcgc cgtacccgtc tgaagagagg
     7021 tagacacagt cgcccgtgcg gagctggatc tcgtggtgtg tgaatgtgcg tgttgtttct
     7081 tcctgcacgc cgccgatcgc gatcttgtca ggacgtatct cgttcagtgt ttccccgctt
     7141 acaacccaca gcgggcggtt ggcgcctgca taatggatca ggccggtggc gttatgaaaa
     7201 acgcataatg acacgtccat tccgtcgcgt gattccccgc cggtattctg cttcaaagcc
     7261 t
//
LOCUS       DASTSF010000124         3045 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_367458,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000124 DASTSF010000000
VERSION     DASTSF010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3045
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_367458"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1686)
                     /locus_tag="VFU15_03675"
     CDS             complement(<1..1686)
                     /locus_tag="VFU15_03675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716901.1"
                     /translation="MHTGIFKRSAVFRALLTVFIAAPFSVNAQVPVPSKCFEIESILV
                     DACVPGGGCTNSASPACNCEGKNEMVRFRTGPSPINTANISINWPNNSYLGIVQNALT
                     ASLTSQLNATIMSCGHLIEPPGGIIPAGKEVLLITSTDMCTTANSFANLADTMYVIFQ
                     NPGNYQGHFANTDNTASITSVPTGASSTRTMIMTYTATGCSDTATYDRSLLVNVFGTY
                     GGSSAQNDGSTVLFSWPGAPVATYVNYGCQAPFVPLSATASGAGPVCSNGNITVSGTP
                     GGNYSSLLWTGGTGTFTSPNSAASAYYPGAGDSGNVVLTFNVIGACQDTAVATVLINV
                     IPVPSAQVTAGGPLTFCNGGNVTLTASGGTTYLWSTGSSSSSISVTAAGTYSVTATNS
                     CGSDTASVNVAVLPLPNAQITAGGPVTFCAGDSVMLSASGGTSYLWSTGSTATSVYVS
                     AAGTYTLTATNSCGNDTATVSVNVTPPPGAQITAGGPTTFCPGDSVLLTASGGSSFLW
                     STGATSASIEVNSAGTYSVTVGGTCGSDTASVTVNTSNNPAGTLSAGGPTTFCT"
     gene            complement(1873..2067)
                     /locus_tag="VFU15_03680"
     CDS             complement(1873..2067)
                     /locus_tag="VFU15_03680"
                     /inference="COORDINATES: protein motif:HMM:NF012534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HEU4716902.1"
                     /translation="MHKGKVKFFNAKNKFGFIVENGTGREFYVHVKDVTGTIAEGDEV
                     EFELEEKKKGPAAVRVKKSA"
     gene            complement(2305..2391)
                     /locus_tag="VFU15_03685"
     tRNA            complement(2305..2391)
                     /locus_tag="VFU15_03685"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2355..2357),aa:Ser,seq:gga)
     gene            complement(2370..2639)
                     /locus_tag="VFU15_03690"
     CDS             complement(2370..2639)
                     /locus_tag="VFU15_03690"
                     /inference="COORDINATES: protein motif:HMM:NF013691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEU4716903.1"
                     /translation="MKLFFAYILRSRKDGRYYYGSTGNLDRRIAEHNSGRVRSAKHRG
                     PFELHYCEMFGSRSEAYRREIFFKTIDGYLWLRENKIILETCPSG"
     gene            2929..>3045
                     /locus_tag="VFU15_03695"
     CDS             2929..>3045
                     /locus_tag="VFU15_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014203742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HEU4716904.1"
                     /translation="MSFHSSIIRVSKVFRFEMAHALSGHDGPCKNIHGHSYSL"
BASE COUNT          674 a          840 c          819 g          712 t
ORIGIN      
        1 cgtgcagaaa gtcgttggtc cgcccgccga taatgtgcct gcaggattgt tcgatgtatt
       61 cacggtgacg gaagccgtat cgctgccgca ggttccgccg acggtgacgg aataggtccc
      121 ggcagaattc acttcaatgg aagccgatgt tgcgcccgtg ctccagagga aactgcttcc
      181 gcctgacgcc gtcagcaaaa cagagtcgcc gggacagaaa gtggtggggc ctcccgcggt
      241 gatctgtgca ccgggcggtg gtgtgacatt gacggaaacg gtcgccgtgt cgttcccgca
      301 ggaattcgtt gcggtgagcg tatacgttcc ggcagctgaa acatacactg aagtcgcggt
      361 cgacccggtg ctccacaaat aagaagttcc gcctgatgcg ctgagcatga cggaatcgcc
      421 ggcgcagaaa gtgaccggcc ccccagctgt gatctgtgca ttgggcaacg gaagaacggc
      481 gacgttcacc gaagccgtat cgctgccgca ggaattggtg gccgtcaccg aataggttcc
      541 ggccgccgtt acgctgatgg aagatgagga agaacctgtg ctccacaggt acgttgttcc
      601 gccggaggcg gtaagcgtta cgttgcctcc attgcagaac gtgagcggtc cgcctgcagt
      661 tacctgtgca gaaggaaccg ggatgacatt gatgagaacg gttgccaccg cggtgtcctg
      721 gcaggctccg atgacgttga acgtaagcac gacgttgccg ctgtcaccgg cgccgggata
      781 atacgccgac gctgcggaat tcgggctggt gaacgttccc gttcctccgg tccacagcag
      841 cgaggaataa tttccaccgg gtgttcccga aaccgtgatg ttgccgttgc tgcagacagg
      901 gccggcaccc gaagccgtgg cgctgagcgg aacgaaaggc gcctggcagc cgtaattgac
      961 atacgtggcg accggcgcgc ccggccagct gaacagcacc gtcgatccgt cattctgcgc
     1021 ggagcttccc ccgtacgttc cgaaaacatt gacgagaagc gacctgtcgt acgtggcggt
     1081 atcagagcat cctgtcgccg tatatgtcat gatcatcgta cgcgttgaac ttgcgcccgt
     1141 gggcacggaa gtaatgctcg cggtgttgtc cgtgttcgcg aaatgtccct ggtaattccc
     1201 cgggttctgg aagatgacgt acatcgtgtc cgccaggttg gcgaacgaat tcgccgtggt
     1261 gcacatgtcg gtcgaggtga tcagcagcac ttctttcccg gcgggaataa ttccacccgg
     1321 aggctctata agatggccgc agctcatgat cgtcgcattg agctgcgaag tgagcgacgc
     1381 cgtgagcgcg ttctgcacaa tgccgaggta ggaattgtta ggccagttga ttgaaatatt
     1441 cgctgtattg atcggtgaag gcccggtccg gaagcgcacc atttcattct tcccctcgca
     1501 gttgcaggcc gggctcgcgg agttcgtgca gccgccgccg ggaacgcatg cgtcgacgag
     1561 aatactttcg atctcgaagc atttggacgg gaccggcacc tgcgcattta cagaaaaagg
     1621 ggcggcgatg aatacggtca gcaacgcgcg aaaaacggcg ctgcgcttga aaattccggt
     1681 gtgcatgatc caggcatttg aaggaaaatt ccgtgaagga tatctatttt ccgtcagcct
     1741 gaagtggttt cggcctgtcg tgccggagca tgacgcctgg aggatgttca cttctgctga
     1801 agcgtcacaa acgtagcaac atatcgttcc tgcagcaagt cccgtttatt aattgtttgt
     1861 aaataggtat gatcacgcag atttcttcac ccttacagct gccggcccct tttttttctc
     1921 ctcgagttca aactctacct catcgccttc agcgatcgtc ccggtcacat cttttacgtg
     1981 cacataaaat tcacgaccgg ttccgttttc cacaatgaaa ccgaatttgt ttttcgcgtt
     2041 gaagaacttg actttcccct tgtgcatatt acaaatgaaa gaaaaacaat tttaaaatgc
     2101 agctattgcg tgggtttaaa gaggaggtta aatcttgccg ggaccagcgg gttcttttca
     2161 ccaacacgaa aaaattacgg aagaaaccgg gatccacttt cctgccgttg caaacggcgg
     2221 cctgcacatt tccgtcagaa agtttctgta aaaaagaagc ccgccattat tcctgctttt
     2281 tgcagggact caggcgggcc ggtgcggaga cggagagatt cgaactctcg atacggtttc
     2341 ccgtatacac actttccagg cgtgctcctt caaccactcg gacacgtctc caggattatt
     2401 ttattctctc tcagccaaag ataaccatcg attgttttaa agaagatttc gcgccggtat
     2461 gcttcacttc tcgatccaaa catttcgcag taatgcaatt cgaaaggccc cctgtgcttc
     2521 gctgacctca ctcttccgga attatgttcc gcaatacgcc gatccaggtt accggtgctt
     2581 ccataatagt agcggccatc tttcctgctc ctgagaatat atgcaaagaa caatttcatc
     2641 ggtcactttc caggtgtgct ctccgaagga gtccttcgga ccttcaacca cccggacacg
     2701 tctcccggta aaagggagcc aaagatacga atagcctcgt ttcggacaaa tgggattttg
     2761 gccggaatac attgtcaaaa ctcccggcct gttttttccc gcccggcatt cataccccgg
     2821 atttgaacct tcagtgaccg ccggttcctt cccaatgatt tccatcacgc ttcgtcccgg
     2881 ccttcatcag tgaagcaggc tttccggcat tctaacttca cgggcgaaat gagttttcat
     2941 tcttccatca tccgcgtcag caaagtattc cgcttcgaaa tggcgcacgc tctttccggt
     3001 cacgacgggc cctgcaaaaa cattcacggg cacagctatt cgctg
//
LOCUS       DASTSF010000125         2622 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1485160,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000125 DASTSF010000000
VERSION     DASTSF010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2622
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1485160"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..773)
                     /locus_tag="VFU15_03700"
     CDS             complement(<1..773)
                     /locus_tag="VFU15_03700"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HEU4716905.1"
                     /translation="MTKYLILIYSGYFICSIFLSFLLNALFLRFSRTLGIRDSPELVR
                     WATTQKPAVGGISFYVLFLLSIACYTVFFQYRLIPLDKKLLGLIGSVTLAFVMGLADD
                     AYNTKPLLKFAVQIACGFILCETGFAIHLFENETLNQALTIFWVVGIMNSINMLDNMD
                     AITASISTVILLEALVSLYVTRNFYSVHVIVILGVVGALGGFLFFNWHPSKLYMGDTG
                     SQFLGILLAAIGIEYFWNSPDYGNNLIFSKRIIVTALGFL"
     gene            884..1423
                     /locus_tag="VFU15_03705"
     CDS             884..1423
                     /locus_tag="VFU15_03705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716906.1"
                     /translation="MKEKLHKTGIVLFCTLFLCGSLLNYPFYRFALNEVKNEMAKEVD
                     RASLAASPDLVKMVFTASEWKKLAGDENEFSVNGNWYDVVSASRAADGGVTVKCLRDS
                     RESLLNSWLKKVTADNGAPANDNGHKNVKSGFSGDYLLSDVLVPVNVSSAGERIAAAN
                     SFPLSNGYLGLPEVPPRIS"
     gene            1517..>2622
                     /locus_tag="VFU15_03710"
     CDS             1517..>2622
                     /locus_tag="VFU15_03710"
                     /inference="COORDINATES: protein motif:HMM:NF017393.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M43 family zinc metalloprotease"
                     /protein_id="HEU4716907.1"
                     /translation="MKSKILLQTVALCALLPFAVNAQQGGFPCGSSYYNDLMKQLHPA
                     EVNAAEQELQQETDYALQHQQDRTTTSYIIPVVFHIVHDYGAENISDAQILDEMMILN
                     RDYAKLNPDTGSIIPQFMTIASETNIEFRLATIDPDGNCTNGIDRIASKKTYQADDAS
                     KLNPWPRQKYLNVWVVNSIGANGVAGYAYYPSSVDGIGLNIDGVLILSNYIGSIGTSS
                     PTTSRALTHEIGHWMNLSHPWGSTNSPGVACGDDGIPDTPKTKGWNHCPTPSTAAICD
                     TGIVENYQNYMDYSYCSCMFTHDQVIAMHATLNSNVSDRNHLWRPDNLVATGTATTTA
                     SSCAPNADFHGNRRMVCPGGTITYTDDSWNGTVSS"
BASE COUNT          657 a          711 c          689 g          565 t
ORIGIN      
        1 agaaaaccga gtgcggtgac aatgatgcgt tttgaaaaga tgagattgtt cccgtaatca
       61 gggctgttcc agaaatattc gatcccgatc gccgcgagca gtatgccgag gaactggctg
      121 ccggtgtcgc ccatgtaaag cttcgacgga tgccagttga aaaaaagaaa tccgccgagc
      181 gcgcccacca cgccgaggat gacgatcacg tgaacgctgt aaaaattccg tgtgacgtaa
      241 agcgagacca gcgcctcgag caggatgacc gtcgaaatgc tggccgttat cgcatccatg
      301 ttgtcgagca tgttgatcga gttcatgatg ccgacaaccc agaaaatggt cagcgcctgg
      361 ttcagtgttt cattttcgaa aaggtgaatg gcgaacccgg tttcgcaaag tatgaatccg
      421 caggcgatct gcacggcgaa cttcagcagg ggcttcgtgt tgtaggcgtc atcggcaagt
      481 cccatcacga acgcaagcgt taccgaaccg atcaggccga gcagcttttt atcgagcggg
      541 atcaggcggt actggaaaaa aacggtgtag caggcgatcg acaggaggaa caggacataa
      601 aaagaaattc cgccgacggc cggtttctgc gtcgtggccc agcgcaccag ttccgggctg
      661 tcgcgtatgc ccagcgtccg tgaaaaccgg aggaacaatg cgttcagtag gaacgaaaga
      721 aagatcgaac agataaagta acccgaatag atgaggataa gatatttggt cattgctatt
      781 tcttacgtaa cggggccgga aaaggatgct tctgaggtca aattgccaca caatgttagc
      841 caacgatttt gaaacgaatg tgatcaattc tatatttgac cgcttgaagg agaaattaca
      901 taagaccggc atcgtgcttt tctgcacgct tttcctttgc ggaagcctgc tcaattatcc
      961 tttctaccgt ttcgcgctca acgaggtgaa gaatgaaatg gcgaaggaag tggaccgcgc
     1021 ttctcttgcc gccagtcctg acctggtgaa aatggttttc accgcatccg aatggaagaa
     1081 actggccggc gatgaaaatg aattttccgt taacgggaat tggtatgacg tggtttccgc
     1141 ttcccgcgct gcagatggcg gggtgactgt aaaatgcctc agggacagcc gcgaaagcct
     1201 tctcaattca tggctgaaaa aagtcaccgc tgataacggc gcaccggcga acgataacgg
     1261 gcacaaaaat gtaaaatcag gcttttccgg cgattacctc ctgagcgatg tgctcgtccc
     1321 cgttaatgtt tcttctgccg gtgagcgcat tgccgccgcc aattcctttc ctctttccaa
     1381 cggctatctc ggtcttcccg aggtgcctcc ccggatttcc tgagctgtaa cggccgggaa
     1441 aaaagcatcc cttctattta ttattcataa cagcatggac aggctgcacc tgtttccatg
     1501 cgtaatacat tcaatcatga aaagtaaaat tttacttcag accgttgcgc tgtgcgcgct
     1561 gctcccgttc gcggtgaatg cgcagcaggg gggatttccc tgcggttctt cctattacaa
     1621 cgacctgatg aaacagcttc atccggccga agtaaatgcg gccgagcagg agctgcagca
     1681 ggaaacggat tatgcgctcc agcaccagca ggaccgcacc acgacatctt acattattcc
     1741 tgtcgtgttt cacatcgttc acgattacgg cgcggaaaat atttccgacg cgcagatcct
     1801 cgacgagatg atgatcctga accgcgatta cgcgaagctg aacccggaca caggttcgat
     1861 catcccgcag ttcatgacca ttgcttccga aacgaatatc gaattccgcc tggcgacgat
     1921 cgatccggat ggcaactgca cgaacggcat cgaccgcatc gcgtccaaaa aaacatacca
     1981 ggccgacgac gcttccaagc tcaatccgtg gccccggcag aaatacctta acgtctgggt
     2041 cgtgaattcc atcggcgcca acggtgtggc gggatatgcc tattatcctt cttccgtcga
     2101 cggcatcggg ctcaatattg acggtgttct tattctcagc aattacatcg ggagcatcgg
     2161 gacgagctcg ccgacgacat cgcgcgcgct gacgcacgag atcggccact ggatgaatct
     2221 ttcgcacccg tggggaagca ccaacagtcc cggcgtggcc tgcggcgatg acggcatacc
     2281 ggacacgccg aaaacaaaag gctggaatca ttgtcccacg ccgtccaccg cagcgatctg
     2341 cgatacgggc atcgttgaaa attaccagaa ctacatggac tattcctact gcagctgcat
     2401 gttcacgcac gaccaggtga tcgccatgca cgcgacgctc aacagcaacg tatccgaccg
     2461 caatcatctc tggcgtcccg acaacctcgt ggccacggga accgcgacga cgaccgcttc
     2521 cagctgcgcg cccaacgcgg atttccacgg caaccgccgc atggtttgcc cgggaggaac
     2581 gattacgtat accgatgatt cgtggaatgg aacggtttct tc
//
LOCUS       DASTSF010000126         2878 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_590564,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000126 DASTSF010000000
VERSION     DASTSF010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2878
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_590564"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1147)
                     /locus_tag="VFU15_03715"
     CDS             complement(<1..1147)
                     /locus_tag="VFU15_03715"
                     /inference="COORDINATES: protein motif:HMM:NF014896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEU4716908.1"
                     /translation="MKNASLFFIGAAFLLASCGGNDASGNKSAFNYPKTRKVDTVDTY
                     FGVKVPDPYRWLEDDNSDSTKKWVDEENALTQDYLAKIPFRDKIRKRLTEIWNFEKMS
                     APYKKGKRYFYSKNDGVQNQSVLYYKDDLNGEGKMLLDPNTLSKDGTVSLGSWDVSHD
                     GKYLAYGLNKAGSDWVDFYVKDIASGKDLTDHIRWSKFSNAAWKDNGFYYGRFEEPKS
                     STYSAENKFQKLYYHKIGDAQDKDQLIYKDDQHPDRSFGAQVTDDQKWLIIYTTETTS
                     GNGLMVKDLTKPNSEFVSIIDNFKNDYGVLDIRDGKMIVRTNNGASNFRLVSIDIAHP
                     EESAWKNIIPEDKNMLEGVTIAGDKFIAHYLVDVKSKLCIFDKDGKNE"
     gene            1310..2323
                     /locus_tag="VFU15_03720"
     CDS             1310..2323
                     /locus_tag="VFU15_03720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716909.1"
                     /translation="MSEENTTVQPAENAPVSSEETVNTEKKEKKELAKIQVQKIYRLL
                     NGEWDDFAELSGPPQRLDDFYLVFERHLTPEGEIMLETELDMEGGEIQKTVNTFNEKG
                     KLVQQELYNEGVLAEKTIYEYDEKDRVVKETREFEEGFPLTAYFKYDDKDRVIEKRVD
                     DSEGELQKRETYTYDETYSDKIATHNVYDEEDNLSLEESFEWMEKDGEVKASKQTVKD
                     HSLNSFRRTEFFDPKEREDNIGYATFNEREKVVEYVKVVFDEKGREAEEHSFSVNDSD
                     NFKVYYTYDDEDRVVMQEQKQEDKIISKIHRRFNTEGHAEIISVRSFSRGIYVDYYEY
                     KYY"
     gene            2476..2808
                     /locus_tag="VFU15_03725"
     CDS             2476..2808
                     /locus_tag="VFU15_03725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716910.1"
                     /translation="MQNENVIGKQNVIGKQIHSNDRLMSAMPVVRTFTADNPPAENLD
                     IIPALEMKLRGMAAINDGSFGIIKLICGENAPVGSGFMNSHDEKNGPVKEEKYSFGDK
                     TPDAVPNA"
BASE COUNT          770 a          650 c          745 g          713 t
ORIGIN      
        1 cttcgttttt cccgtctttg tcaaaaatgc agagcttcga tttcacatcg acgaggtaat
       61 gcgcaatgaa cttgtcgccg gcgatcgtca cgccttcgag catgttcttg tcttcgggaa
      121 tgatgttttt ccaggctgat tcctccgggt gtgcgatgtc aatggaaacg aggcggaaat
      181 tagacgcgcc attgttggtg cgcacgatca tcttcccgtc gcggatgtca agcacgccat
      241 aatcattctt gaaattatcg atgatggaga cgaactccga atttggtttt gtaaggtctt
      301 tcaccatcag tccattcccg ctcgtggttt cggtggtgta aatgatgagc catttctggt
      361 cgtctgttac ctgcgcgccg aagctcctgt caggatgctg atcatccttg taaatgagct
      421 ggtctttgtc ctgagcgtcg ccgatcttat gataatacag cttctggaat ttgttttccg
      481 cggaatacgt ggaggatttc ggttcttcaa accgcccgta gtaaaaacca ttatccttcc
      541 aggcggcgtt ggaaaatttg ctccaccgga tgtgatccgt aagatcttta ccggaagcga
      601 tatctttcac gtaaaaatcc acccagtcgg acccggcttt gttgaggccg taggcaaggt
      661 attttccgtc atgcgaaaca tcccagcttc ccagcgaaac cgttccgtcc tttgaaagcg
      721 tattcgggtc gagcagcatt tttccttcac cattgagatc atccttgtaa taaagcacgc
      781 tctggttctg aacgccgtca ttcttcgaat agaaataacg tttccctttt ttgtagggcg
      841 cgctcatctt ttcaaaattc caaatttcgg tcaggcgctt gcggatcttg tcgcggaagg
      901 gaatttttgc gaggtaatcc tgcgtcagtg cattttcttc gtcgacccat tttttagttg
      961 aatcggaatt gtcatcctcg agccagcggt acggatcagg aacttttacg ccgaagtacg
     1021 tgtcgaccgt atcgaccttc cttgttttcg ggtaattgaa tgcgcttttg tttccggaag
     1081 cgtcgttccc gccgcaggaa gcgagcagga aggcagcacc gataaagaag agggaagcat
     1141 ttttcattgg cgggaattgt tggaagtcca aatgtaagca aagaaggatt ccccagaagc
     1201 gcattgggac gggtggaaaa ataggatttc agccggtcgg gcagaagcgt ttttttacac
     1261 tttccccaat gaatattatt tctaaattta agtcttaatc aaaccgaata tgtccgaaga
     1321 aaacaccacc gttcagcctg ccgaaaatgc ccccgtatcg tccgaagaaa ccgtgaacac
     1381 ggaaaagaaa gaaaagaagg agcttgcgaa aatacaggtc cagaaaattt accgcctgct
     1441 caacggggag tgggatgatt ttgcggagct gtccgggccg ccgcagcgac tggatgattt
     1501 ttacctcgtg ttcgagcgcc acctgacgcc ggaaggagag atcatgctgg aaacggagct
     1561 ggacatggag ggcggggaga tacagaaaac agtgaacacg ttcaatgaaa aaggaaaact
     1621 ggtacagcag gagctgtaca acgaaggcgt gctggcggaa aaaacgatct acgagtacga
     1681 cgaaaaagac cgcgtggtga aagagacacg ggagttcgaa gagggatttc cgctcactgc
     1741 ttatttcaaa tacgacgaca aggaccgcgt gatcgagaag cgcgtcgacg acagcgaagg
     1801 tgaattgcag aaacgcgaga cgtacacata cgatgaaaca tacagcgaca agatcgcgac
     1861 gcataatgtg tatgacgagg aagacaatct ttccctcgag gaaagtttcg aatggatgga
     1921 aaaggacggg gaagtaaaag cgagcaagca gacggtgaag gatcattcgc tgaattcttt
     1981 ccgccgcacg gaattttttg atccgaaaga gcgcgaagac aacatcggtt acgcgacgtt
     2041 caacgagcgc gagaaagtgg tggaatacgt gaaagtggtg ttcgatgaaa aaggacgcga
     2101 agcggaagaa cattctttct ccgtcaatga ttcggataac ttcaaagtgt attacacgta
     2161 cgatgacgag gaccgcgtgg tgatgcagga acagaagcag gaagacaaga tcatcagcaa
     2221 gatccatcgc cgcttcaaca cggaaggtca tgccgaaata atttccgtgc gctcgttcag
     2281 tcgcggcatc tatgtcgatt actacgagta taaatattac tgaagcgcat gttctgtcgt
     2341 ttgatcccgg cgtatcattc tttcttaccg gcgcattccg tctgcatttt tcatccggcg
     2401 acctcaatct tcacgcaatg aaatattccg cgggtggatc tgcccggctt gtgaatattc
     2461 cgtttgtgag ggaatatgca gaatgaaaat gtcattggta aacaaaatgt cattggtaaa
     2521 caaattcatt ctaatgaccg tttaatgagc gccatgcccg tcgttcgtac tttcactgcg
     2581 gataaccctc cggccgaaaa ccttgatatc attcctgcgc tggaaatgaa gctccgcggg
     2641 atggctgcta ttaacgatgg cagtttcggg ataataaaac tgatttgcgg ggaaaacgca
     2701 ccggtcgggt cggggttcat gaattctcat gatgaaaaaa acgggccggt gaaagaagaa
     2761 aaatattcct tcggggataa aacacctgat gctgttccca acgcatgaaa gagatcgaca
     2821 acataccgct cgtgtttgtc ctttcgaaag aacggtcggg aaccacgctg ctgcaggt
//
LOCUS       DASTSF010000127         8128 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_150357,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000127 DASTSF010000000
VERSION     DASTSF010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 8128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8128
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150357"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2177)
                     /locus_tag="VFU15_03730"
     CDS             complement(<1..2177)
                     /locus_tag="VFU15_03730"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEU4716911.1"
                     /translation="MNFTVYRSSAGSGKTFTLVREYLAIALSDTAFPPQRYKEILAIT
                     FTNKAAGEMKERIIRALKELSGKPAEISSPLSSALQEKLMLDPFTLAERAGNVLKAIL
                     HNYGDFAIGTIDSFTHRIVRAFAHDLSLPVNFEVETDEEKIISEAVDLLVSRIGKDEM
                     LTEILYSFSESRTEDEKNWQIESDLRDISRQLLREDGADFASRLRDLTPENFMTIRKK
                     LLTFIRQFESKVIALASELHQRFRDHGLEEEDFAHGKTGIAGRIRAFANGRLEKFGDG
                     NKNVTKTLASGKFHSTKATAAKVAAINALVPEITSVFAQMDALQEKEFGDYILRGMML
                     KNIFALAVLNEIEKIIFSFRTEDNIIHISEFNRIISRVVAGEPAPFIYERLGERYRNY
                     LIDEFQDTSVTQWHNLLPLIENALGGNNFTMVVGDSKQAIYRWRSGDVEQFARLPAVP
                     SAQDSELLLQREASLKRNYREEKLGTNFRSKSEIVNFNNGFFRKLSEETGPYAEEIYR
                     GIEQEFRKENAGGYVRIEAVPEEEERNANAVHLEKTITLIRDLLAEGFRHSDIAVLTR
                     TNREGSETAKLLVENGIPVLSPDSLLLQHSAAVGFVVSILKIIENPSDEIATASVTEF
                     LVRFGKTSGTYHENLAATTEPGGLAALCAANGIAFEPETLSRRPLYQRCEEIISVFGL
                     SSSLDSYLLFFLDEVLSWSSSKSASRTDFFTWWDDRRQRASVV"
     gene            2396..4111
                     /locus_tag="VFU15_03735"
     CDS             2396..4111
                     /locus_tag="VFU15_03735"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEU4716912.1"
                     /translation="MKRSLFFSAFILFLLSRVPAQAPVNNPVVPDDEHLAYGVILHPG
                     EYVPNQLLVMFAPGITAGEAEEKFASAGFVVVQDHETAKSMRIHTLTFTSGTDIEKAL
                     AFAKNIPEIETAQFNHYVAHRSNVPDDPQFPGMWDMNNTGQTGGTPDADIDAPEAWDI
                     ATGGLTVQGDTIVVAVIDAGFDLAHQDLDFWKNRGEIPNNGIDDDGNGYIDDYDGWNA
                     YNSNGTISSDVHGTHVSGTVGAKGNNSTGVVGVNWNVKIMPVMGSDGTEAQVVEAYTY
                     VFDARRLYNTTNGAQGAFVVATNSSFGIDQGQPANYPLWCAMYDSLGSVGILNAAATA
                     NQNWDIDAVGDVPTACASPYMIAVTNTTMTDSKNTYAGYGATTIDLGAPGTYIVSTVP
                     GNSYGTLTGTSMASPHVAGAIALMYSAACSQLISDYKTSPDSVALLMRNYLLAGTDPV
                     SSMNGVTTSGGRLNLFGALTQVQTYNCGPGGIAVHPVLQEPEIYPNPSAGDLTMIIPS
                     SSAGTAQIVFSDVTGRVVYSSSAELSAGTSTVTFHSLPLAAGTYFISVITDDGTFSPE
                     QVVIR"
     gene            complement(4127..5437)
                     /locus_tag="VFU15_03740"
     CDS             complement(4127..5437)
                     /locus_tag="VFU15_03740"
                     /inference="COORDINATES: protein motif:HMM:NF028132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716913.1"
                     /translation="MTPGKNIHRLFVIVFMTVLLIPLAQECTRLIRLAPLDGAFVPAK
                     KPLLSVSAFWNGTYQDSFETWQNENFGCRNFYVRFHNQLQYELFHKASVDNVTVGLQD
                     YIYENQYITTTFGEDFIGDEAIGNVIYKLKCVQDTLAKMNKTFVLVICPGKGTYYPEY
                     IPFKKPTVHWPTNYEVFTDKLKNSGLNYIDFSSWFRAMKDTSKAPLIPKTGSHWSVYG
                     SMLALDSFIRYVEVKRNVDLPDLQLHGFTWKTSNLTPPDEDVCVSMNLLWNIRPLPMA
                     YPDYSFGDATGKAKLNLMAVSDSYFWIWNDAGIGRNCFHRSEFWYYDQHVYYSDNTPE
                     GNALDLDDVEATKNNDVIILMATEPNLSGLGWNYINDVYDRLVLKKVPTDMDKRIREV
                     ETGIRKSPEWMKSIVEKAAMNKISIDSMIYLDAKYVIEQQDQKK"
     gene            complement(5437..6882)
                     /locus_tag="VFU15_03745"
     CDS             complement(5437..6882)
                     /locus_tag="VFU15_03745"
                     /inference="COORDINATES: protein motif:HMM:NF015049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HEU4716914.1"
                     /translation="MVFSSSIFLTYFLPIVLLVYFLLPKKARNTFLLFASMVFYGWKA
                     PRFIFVILATTLLDFFLVNEMWKSDVKLRRRLFLTLSVGVNLGMLVYFKYSGFFVDNI
                     NSLIGVFGGNAIKWSKVVLPIGISFYTFESITYVVDVYRKIHAPQKKFTSYLLYIIFF
                     PKLVAGPIIRYHEIADQLEERNETNERRITGFIRFCTGLGKKVLIANYLGLTADYVYG
                     PMDNAAAGLDPHSLTLSLSWAAALAYTFQIYFDFSGYSDMAIGLARMFGFDIPENFNQ
                     PYLSESITDFWRRWHITLGAWMKNYLYIPLGGNRSGPVRVYLNLFIVFLISGLWHGGA
                     WNFIVWGCWHGFFLVIERLFLLKLYEKLWRPLRILVTFFIVLIGWVFFRVENIERAFR
                     YVEALFVHGSGKNYYQGDTQTLFFFALAAFFSFFSFFGFSAKWCREWFAGGFTKTWKL
                     SAALLTSLLLLFISMSYLTVANFNPFIYFRF"
     gene            complement(6883..7431)
                     /locus_tag="VFU15_03750"
     CDS             complement(6883..7431)
                     /locus_tag="VFU15_03750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716915.1"
                     /translation="MRYSEAPLILRILAWPVIAGCGFVVFHALMLYLGYDAETWVKEI
                     YITLQNGLPFFLFLTGSFVRDKKFRIPLLLFFPLFIACYFINMLYEYDVIGFGVTQWI
                     IGPAELGLFLTYLFFFILEKKKNVTSVLKILWFFCITWAYLVPKFTTGFHAGSFFLAS
                     IFIFPFLMGFGLYDFFRKPIAS"
     gene            7765..>8128
                     /locus_tag="VFU15_03755"
     CDS             7765..>8128
                     /locus_tag="VFU15_03755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716916.1"
                     /translation="MRISGELEELVSTLSKPEKRHIQLTASMHGGEKNYVKLLSELTK
                     KNPKKKKQKKKAGDPDTRRYLFSLVLKSIGSYYPDKSIDAEINELLISAEVLLDRNMM
                     SARKKILDRAKRMAYRYAK"
BASE COUNT         1992 a         1976 c         2267 g         1893 t
ORIGIN      
        1 ataacgacag aagcgcgttg cctgcgatcg tcccaccagg tgaaaaagtc agtgcgggaa
       61 gccgatttgg aggaagacca gctgagcact tcgtcgagga agaaaagcag gtaggaatcg
      121 agggaagagg agagaccgaa aacggaaatg atctcttcgc atcgctggta aagcgggcgg
      181 cgtgaaagcg tttccggttc gaacgcaatt ccgttcgccg cacagagtgc ggcaagtccg
      241 ccaggttcag tggttgcggc gaggttttcg tggtaggtcc cgctggtttt tccgaaacgg
      301 acaaggaatt cagtaaccga agctgttgca atttcgtccg acggattttc gatgatcttc
      361 aggatcgata ccacgaagcc gaccgctgcc gaatgctgga gcaggagcga gtcgggagag
      421 agcacgggaa tgccgttttc caccagcagc tttgcggttt cgcttccttc gcggttggtg
      481 cgcgtgagca ccgcgatgtc gctgtgacga aaaccttccg ccaggagatc gcgtatgagc
      541 gtgatcgtct tttcgagatg aacggcattt gcgtttcgtt cttcttcttc cggcacagct
      601 tcgatgcgga cgtagccgcc tgcattctct ttccggaatt cctgctctat gccccggtat
      661 atctcttccg catacggacc tgtttcttcc gacagcttcc tgaaaaaacc attgttgaag
      721 ttcacgatct ctgatttcga acggaaatta gtgccgagct tttcttcgcg gtaattcctt
      781 ttcaggcttg cctcgcgctg gaggagcagc tccgaatcct gtgcagacgg aactgcaggg
      841 agcctcgcga actgttccac gtcgccgctt cgccagcggt agatcgcctg cttgctgtcg
      901 ccgacgacca tggtgaaatt atttccgccc agcgcgtttt cgatcagcgg aagaaggttg
      961 tgccattgcg tgacggacgt atcctggaat tcatcgatga ggtagttgcg gtaacgttcc
     1021 ccgagacgtt cgtagatgaa cggggccggt tctcccgcca ccacgcgcga gatgatgcgg
     1081 ttgaattcgg aaatgtggat gatgttgtct tccgtacgaa agctgaagat gatcttttcg
     1141 atctcgttca gcacggcgag ggcgaatatg tttttcagca tcatcccgcg aaggatgtaa
     1201 tcgccgaact ctttttcctg caacgcatcc atctgtgcga atactgaagt tatctccgga
     1261 acgagtgcgt tgatcgctgc gactttcgct gccgtcgctt ttgttgaatg gaatttcccg
     1321 gaagcgaggg ttttcgttac atttttattt ccgtcgccga acttttccag gcgcccgttt
     1381 gcgaatgcgc gtattcttcc ggcgatgccc gttttcccat gggcgaaatc ttcttcttca
     1441 agtccatgat cgcggaaacg ctggtgaagt tccgatgcca gcgcaatgac cttcgattca
     1501 aactgccgga tgaaagtgag cagtttcttc cggatcgtca tgaaattttc cggggtaagg
     1561 tcgcgcaggc gagaggcgaa atccgcacca tcctcgcgaa gcagctgtcg cgaaatatcg
     1621 cggagatcgg attcgatctg ccagttcttt tcatcttccg tacgcgactc cgaaaaactg
     1681 tagaggattt ccgtcagcat ttcgtctttc ccgatgcggc tgacgagcag gtcgacggct
     1741 tccgaaataa ttttttcctc gtccgtttcc acttcgaaat tcacgggaag actgaggtca
     1801 tgcgcaaatg cgcgcacgat gcggtgcgtg aagctgtcga tcgtcccgat cgcgaagtcg
     1861 ccatagttat ggaggatcgc cttgagcaca ttgccggcgc gttccgccag cgtgaacgga
     1921 tcgagcatca gtttttcctg cagggccgat gacagcgggg atgagatctc tgccggtttc
     1981 ccggaaagtt ctttcagtgc gcggataatg cgctccttca tttcgcctgc cgctttattg
     2041 gtgaaggtga tcgcaagaat ttccttgtat cgctgcggtg gaaaagccgt atcggaaagc
     2101 gcgatggcga ggtactcgcg caccagcgta aatgttttgc cggaaccggc ggacgacctg
     2161 tagaccgtaa agttcacgag agtaaatgta aggaacgtgc gggatgtttg tccgcatccg
     2221 cgttttcagg tttgtcgtgg cggatcttca gcgttcacct ggaacccgga actttcaaac
     2281 ttttaatttg agccgttttc cgtttgttgt gcgtcgctcg tcgtttggga tttgaacaac
     2341 gccgcggcat aggaaacact gtcttttttg agtaaatttg aagaaggccg tattcatgaa
     2401 acgatccctt ttcttttctg cattcatcct gttcctgctt tcacgggttc ccgcccaggc
     2461 gccggttaat aatcctgttg tgcccgatga tgagcatctt gcctacggcg tcatccttca
     2521 cccgggcgaa tatgttccca accagttgct cgtgatgttt gcgccgggta tcaccgcagg
     2581 cgaagcggaa gaaaaattcg cgtcagccgg atttgttgtg gtgcaagatc atgaaacggc
     2641 gaaaagcatg cgcattcata cgctgacatt taccagtggg acggatattg aaaaagcgct
     2701 tgcgtttgcc aaaaacatcc cggaaataga aactgcgcag ttcaaccatt atgtcgcaca
     2761 caggagcaac gtgcccgacg atccgcagtt ccccggaatg tgggacatga acaatacggg
     2821 acaaacaggc ggcacgccgg acgcggatat agacgcgccg gaagcgtggg atatcgcaac
     2881 cggcggactc accgtccagg gtgatacgat cgtcgttgca gtgatcgacg caggttttga
     2941 cctggcgcac caggatctcg atttctggaa gaaccgcggg gaaatcccga acaacggcat
     3001 tgatgatgac ggcaacgggt acatcgatga ttacgacgga tggaacgctt acaacagcaa
     3061 cggaacaatc tcgtcggatg ttcacggaac gcatgtgtcc ggaacggttg gcgcgaaagg
     3121 aaataattcg accggtgtgg ttggcgtgaa ctggaatgtg aagatcatgc cggtgatggg
     3181 ttccgacggg accgaagcgc aggtggtgga agcgtatacg tatgttttcg atgcgcgcag
     3241 gttgtacaat acgacgaacg gggcgcaggg cgcttttgta gtggcgacga attcttcttt
     3301 cggcatcgac cagggacagc cggcgaatta cccgttatgg tgcgcgatgt acgattcgct
     3361 gggttccgtg ggcatcctga atgctgctgc aaccgcgaat caaaactggg acatcgatgc
     3421 ggtaggagac gttccgacgg catgcgccag tccgtacatg atcgcagtga cgaatacaac
     3481 aatgacggac agcaaaaaca cgtatgcggg ctatggcgca acaaccatcg atctcggcgc
     3541 gccgggaacg tatatcgttt caactgttcc gggaaattcc tacggcacgc tcaccgggac
     3601 gtcgatggct tctccgcacg tggcgggtgc aatcgcgctc atgtattcgg cggcatgttc
     3661 gcagctgatc agcgattaca aaacatcgcc ggacagcgtc gcattgctga tgcggaatta
     3721 ccttctcgcc gggactgatc cggtcagcag catgaatggt gtaacgactt ccggcgggcg
     3781 cctgaatctt ttcggcgcgc tcacgcaggt gcaaacgtac aactgcggtc cgggcggaat
     3841 tgcagtccat cctgttttgc aggagccgga aatttatccc aatccttccg ccggtgatct
     3901 tacgatgatc attccttctt cttcagccgg aaccgcgcag atcgtttttt ccgacgtcac
     3961 ggggagagtg gtatattctt cttccgctga gctttctgcc gggacatcga cggtcacgtt
     4021 ccattctctt ccgcttgcag cgggaacgta ttttatttct gtgatcacgg acgatggaac
     4081 gttttccccg gagcaggtcg ttatccggta agaaaaaaaa acggatttat tttttctgat
     4141 cctgctgctc gatgacgtat ttcgcatcga gatagatcat agagtcgatg gagattttgt
     4201 tcatcgctgc tttttcaacg atgctcttca tccattccgg gcttttacgg atacccgttt
     4261 ccacttcacg gatgcgtttg tccatgtcgg tcggaacttt tttcagtaca aggcggtcgt
     4321 aaacatcatt gatataattc cagccaagtc ctgaaagatt cggttccgtg gccatcagga
     4381 tgatgacatc gttgttcttt gttgcttcca catcgtcgag atcgagcgca ttgccttccg
     4441 gtgtgttgtc ggagtaatac acatgctggt cgtaatacca gaattcactg cggtgaaagc
     4501 aattcctccc tataccggcg tcgttccata tccagaaata actgtcggaa accgccatca
     4561 ggttcagctt cgcttttccc gttgcatccc cgaagctgta atcgggatag gccatgggca
     4621 gcgggcggat gttccagagg aggttcatcg aaacgcacac atcttcgtcc ggcggtgtca
     4681 ggttgcttgt tttccatgta aacccgtgga gttgcagatc gggaagatcg acgttgcgct
     4741 ttacttctac gtaacggata aagctgtcga gcgcgagcat ggacccgtac acgctccagt
     4801 gcgaaccggt tttgggaatg agcggagctt tcgatgtgtc cttcatcgcg cggaaccagc
     4861 tactgaaatc aatataattg agcccggaat ttttcagctt gtccgtgaat acttcatagt
     4921 tcgtcggcca gtgcacggtt ggttttttga acggaatata ttccgggtaa tacgttcctt
     4981 tcccgggaca gatgacgagc acgaacgttt tgttcatttt cgccagcgta tcctgcacgc
     5041 atttcagctt gtaaataaca ttcccgatcg cttcgtcccc gataaaatcc tccccgaacg
     5101 tagtggtgat gtactggttt tcgtagatgt agtcctgcag accgacagtg acgttatcca
     5161 cagaagcttt atggaacaat tcatactgca attgattatg aaaacgaacg tagaaattcc
     5221 tgcacccgaa attttcattc tgccatgttt caaagctgtc ctggtaggtt ccgttccaga
     5281 atgcggaaac ggaaagcagc ggtttttttg cgggaacgaa tgctccgtcc agcggggcga
     5341 gcctgatcag ccgggtgcat tcctgggcaa gcggaatgag gagcaccgtc atgaaaacga
     5401 tgacgaacag gcgatggata tttttgccgg gcgtcatcag aacctgaaat agatgaaggg
     5461 attgaaattc gccacggtga ggtagctcat ggaaatgaaa agaagcagga gagatgtgag
     5521 caaagcagcg gaaagcttcc atgtttttgt gaacccgccg gcgaaccatt cgcggcacca
     5581 ttttgcgctg aacccgaaga acgagaagaa agaaaagaag gcggcgagcg caaaaaagaa
     5641 taacgtctgc gtgtcaccct ggtaataatt cttaccgctg ccgtgaacga aaagcgcttc
     5701 gacgtaccgg aatgcccgct caatattctc cacgcggaaa aacacccaac cgatgaggac
     5761 aatgaagaac gtgaccagga tgcgcagcgg ccgccacagc ttttcataga gcttcagcag
     5821 gaacaggcgt tcgatgacaa gaaaaaatcc gtgccagcat ccccacacga tgaagttcca
     5881 cgcgcctccg tgccagagac cggaaatgag gaacacgatg aacaggttga ggtacacgcg
     5941 cacgggcccg gaacggtttc cgccgagcgg gatgtagagg taatttttca tccacgcgcc
     6001 gagcgtgatg tgccagcgcc gccagaaatc ggtgatgctt tcggagagat aaggctggtt
     6061 gaaattttcg ggaatgtcga agccgaacat gcgcgcgagg ccgatggcca tgtcggaata
     6121 gccggagaaa tcgaaataga tctggaacgt atacgcgagg gcggctgccc aggagagcga
     6181 gagcgtgagg ctgtgcggat cgagaccggc cgcagcattg tccatcggac cgtaaacgta
     6241 atcggctgta agaccgaggt aattcgcgat gagcactttt ttccccaggc ctgtgcagaa
     6301 gcggatgaaa cccgtgatgc ggcgttcgtt cgtttcgttg cgttcctcca gctggtcggc
     6361 gatctcgtgg tagcggatga tcgggccggc gacgagcttc ggaaagaaga tgatgtaaag
     6421 aagatacgac gtgaattttt tctgcggtgc atgtatcttc cggtatacat cgaccacgta
     6481 cgtgatgctt tcgaaggtgt agaacgaaat gccgatcggg agaacaacct tcgaccattt
     6541 gatcgcgttg ccgccgaaga cgccgatcag cgaattgatg ttgtcgacga agaagccgga
     6601 gtacttgaaa taaacgagca tacccaggtt gacgccgaca gaaagtgtga ggaacagtct
     6661 tcgccggagc ttcacgtccg acttccacat ttcattgacg agaaaaaaat caagcagcgt
     6721 ggtggcgagg atcacgaaaa taaaacgggg agctttccat ccgtaaaaga ccatgctcgc
     6781 gaaaaggagg aacgtgttcc gcgctttttt cggaaggaga aaatacacca gcagaacgat
     6841 gggaagaaaa taggtgagaa agatcgagct gctgaaaacc attcaggatg caattggttt
     6901 tctgaaaaaa tcatacagcc cgaagcccat gaggaacggg aaaatgaaga tggacgcgag
     6961 gaagaagcta cccgcgtgaa agccggtggt gaatttggga acgaggtacg cccaggtgat
     7021 gcagaagaac cagaggatct tcaataccga tgtgacgttt tttttctttt ccaggataaa
     7081 aaagaaaaga taggtgagga agagcccgag ttccgccggc ccgatgatcc actgcgtcac
     7141 accaaagccg atcacgtcgt attcgtacag catattgatg aaatagcagg cgatgaagag
     7201 cggaaaaaac agcaggaggg gaatgcggaa ttttttgtcg cgtacgaatg agccggtgag
     7261 aaagagaaag aaaggcagcc cgttctggag cgtgatgtag atttccttca cccacgtttc
     7321 ggcgtcgtag ccgaggtaaa gcatgagcgc atgaaagacc acgaaaccac agccggcaat
     7381 aacaggccag gcaagtatgc gtaagatcag cggagcttcg gaatacctca ttgcagagaa
     7441 acacaaaaat aggaataccg gggagaaagg ggatataagt ttcgggttac gggtttcggg
     7501 tttgaggttg ctgaaaaata aaaacaccgg tcgccgcaat ggcggagatg aaatggcttc
     7561 caggcttggg ttaaatcatt cccggcgcgc gggaccggtg ctgagaagcg gttgctgctg
     7621 tcgttccggc ttcacgcggc aaatgtatcg ggcggaaatc gcatcgccca ggcaaggttt
     7681 cgcacaaaag gtttttttag ctggccgtat tatttgtatt tttctgaata acaattaatt
     7741 gtaattaatt aaacggcaat tccaatgcgt atttctggag agctggaaga gcttgtttcc
     7801 acgctgagca agccggagaa aagacacatt cagctgacgg cttcgatgca cggcggagaa
     7861 aaaaattacg tgaagctgct cagcgagctc acgaaaaaaa atccgaagaa gaagaaacaa
     7921 aaaaagaaag caggcgatcc cgatacgcgg cgttacctct tcagccttgt gctgaaaagc
     7981 atcggcagct attatcccga caaatcgatc gatgcggaaa taaatgaatt gctcatcagc
     8041 gcggaagtat tgctcgaccg caacatgatg agcgcgcgga aaaagatcct cgaccgcgca
     8101 aaacgcatgg cctatcgcta tgcgaaat
//
LOCUS       DASTSF010000128         4932 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_812699,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000128 DASTSF010000000
VERSION     DASTSF010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4932
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_812699"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..479
                     /locus_tag="VFU15_03760"
     CDS             <1..479
                     /locus_tag="VFU15_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020876502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716917.1"
                     /translation="TFLLSGLWHGASWTFVAWGALYGIIYLLEIPVLKFTQGKNWSRF
                     PGWIYLVSAHSLIMVAFRAGSISDTWTFYSKIFSFDWNAGSSFAELKHLNDLFPLVLS
                     VFLVVFLFVKETQEEFFPLREGKTGSILRPALYVMIFLGIFLIGQFNANEFIYFHF"
     gene            482..1417
                     /locus_tag="VFU15_03765"
     CDS             482..1417
                     /locus_tag="VFU15_03765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716918.1"
                     /translation="MKRAAFYILLVTALAWLMREAEYEGIRRNREGQFDKFNTCFARK
                     NNFDLIIIGSSRAESHFRPDIIDSATGFHSYNAGMVGATLPFIEGTLEAYLENSAPPK
                     YVVLNIDYHMFIASDDTIRYFPGYFPYLGNHALYEKFRERDPRFTYFKWIPFYSLPYL
                     GSKYFDNALHGWFNVPGRYDSTYIRGYTPIAAASPADIDTITIKPYSPWLKESSWKSL
                     DRIISICHEKNIKLIFVLSPLYHRLSEAIVNEKTIIARFHEIAVKNGFVLMDYSHTFI
                     SEDKTLFSDLDHMNRRGALYFSRLFSRDLAQYARR"
     gene            1464..2564
                     /locus_tag="VFU15_03770"
     CDS             1464..2564
                     /locus_tag="VFU15_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HEU4716919.1"
                     /translation="MKRFRTSFFLVFLLGLSVQSFAQIRKYSNEFLAIGVGARSLAMS
                     GASVASADDVTSGYWNPAGLLGVKPDFQVGLQHAEYFASIAKYDYLGFAGKIDSASSF
                     GFSVLRFAVDDIPNTTQLIDPSGNINYDNITTFSAQDYAFLISYARKAPIAGLHYGAN
                     VKIIRRKVGDFAGAWGFGLDAGAQYDYKGWRLGAVVRDVTSTFNAWSFNLSQDMKDVF
                     AATGNEIPQNSLELTMPRLIFGASKTYPVGRQQKFSVGGEMDFINTFDGKRNVLVRTG
                     LWSMEPVLGLEAGFKNIIFLRTGIGNIQKSKDVFGKDVTTFQPNLGVGLRIKSLTIDY
                     AFTDIGNQSVALYSNIFSLKIDINKKKIPGGS"
     gene            2599..>4932
                     /locus_tag="VFU15_03775"
     CDS             2599..>4932
                     /locus_tag="VFU15_03775"
                     /inference="COORDINATES: protein motif:HMM:NF013524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HEU4716920.1"
                     /translation="MKQFYRKAFFSSTGIFFFLFFLFPPDVSSQTYNNSWISYSQHYL
                     KIKVVNDGVYRIDSTVLSGAMNSMGIPLSSVDPRNFQVFHNGQEQYIWVQGESDGVFN
                     AGDYIEFLGYRNDGRPDSSLYASPDSLLDPYYSLYNDTSVYFLTWNNLFTNLRMTASN
                     DTAFSAYTPADYFTHSEIMRGTGTYMPGVADALGITDPAFIPSEGYYTGVSYYAQPVT
                     YNLSSANAYTGPGAPNAQLSMKVGTFSNDWNVANDNDVRIQYPGGPVFDSIYDGFAVG
                     SYHFAVAAASLGAAAPVTVTSVNSNGSSSSGLIGTAFINLTYPHTFDMESRTGMYGTL
                     PDNTTGPKTLVKFSNLSGAAPARVYDLYNHQRIDAVQNGIYYYALVQNGNGSEKPVLF
                     SSEANVVNVASVQPENGSGLFTDFSQQAADSVFIIITHRSLMGVGTQYAAYRSSIAGG
                     SHNVVLADVNELYDQFAWGIGEDPLAIRNFCAFMIGTFPTPPQDLLLLGKSYYADYAR
                     LYGYYGVNLVPTMGYPPCDNSLTAGLNGTLWEPAIPTGRIAAADSAQAQWYLNKVIEY
                     ENNQPAEWMKYVLHFGGGTSTSEQQAYKSYLQNYQHTIEDTLFGGYVQTYLKNSSAPI
                     QINQSDSLRQRIEDGVSIMTFFGHASGTGFDQSIDDPSTYNNFDRYPFLIANSCYAGD
                     IHSDGISSSEAFTLIDQKGTIGYLASVGVGIPQYLDMYTSRLYKSVGQVNYGQPVGKC
                     IQWTVHNAETTQQQSIYLKTTCMEMTLQGDPAIVINSF"
BASE COUNT         1086 a         1459 c         1222 g         1165 t
ORIGIN      
        1 tcacgttcct gctcagcggc ctctggcacg gcgccagctg gacgttcgtc gcgtggggcg
       61 cgctctacgg catcatttac ctgctggaaa ttcccgtgct gaaattcacg cagggaaaaa
      121 actggtcgcg gtttcccggc tggatctacc tcgtgtccgc gcattcgctg atcatggtcg
      181 cgttccgcgc aggcagcata agcgacacgt ggacgttcta ttcgaaaata ttttcctttg
      241 actggaacgc aggctcctcg ttcgcagagc tgaaacacct gaacgatctt ttcccgctcg
      301 tgctttcggt gttccttgtt gtttttcttt ttgtcaagga aacgcaggaa gaattttttc
      361 cgctgcgcga gggaaaaaca ggaagcattc tgcgtcccgc gctttacgtg atgatcttcc
      421 tcggcatttt cctgatcggg cagttcaatg caaatgaatt catctatttc catttctgaa
      481 aatgaaacgc gccgctttct atattcttct cgttaccgcg ctggcctggc tgatgcgtga
      541 ggcggaatat gaaggcatcc gcaggaacag ggaaggacag ttcgataaat tcaatacctg
      601 cttcgcccgt aaaaacaatt tcgatctcat catcatcggc tcgtcacgcg ccgaatctca
      661 tttccgtccc gacatcatcg acagcgccac ggggtttcat tcctataacg cgggcatggt
      721 gggcgcaacg cttcctttca tcgaaggaac gctcgaagcg tatctcgaga acagcgctcc
      781 gccgaaatac gtcgtgctga acatcgatta ccacatgttc atcgcttccg acgatacgat
      841 ccgttatttt ccaggctact ttccttacct cggcaatcat gcgctctatg aaaaattccg
      901 cgagcgcgat ccccgcttca cgtatttcaa atggatcccg ttctacagcc ttccctatct
      961 cggcagcaaa tatttcgaca acgccctcca cggctggttc aacgtcccgg gccgttacga
     1021 ttccacatat atccggggtt acacgcccat tgctgccgcc agcccggcgg acatagacac
     1081 gatcacgata aagccgtaca gtccgtggct gaaagaatca tcctggaaaa gcctcgaccg
     1141 catcatttcc atctgccacg aaaagaacat taagctcatc ttcgttcttt ccccgcttta
     1201 ccaccggctt tcggaggcga ttgttaacga aaaaacaatt atcgcgcgct ttcatgaaat
     1261 tgccgttaag aacggtttcg tgctgatgga ttattcgcac acattcatct cggaagacaa
     1321 aactcttttc tccgatctcg atcacatgaa ccgcaggggc gccctgtatt tttcccgcct
     1381 cttttcccgc gatcttgcgc aatatgcccg ccgctgaggc gtttttgtga ggtcaaaagc
     1441 tttgccttaa ctttatgaat cgaatgaaac gcttccgtac ctcttttttt ctcgttttcc
     1501 tgctcggcct ttcggtgcag tcgtttgcgc agatccgcaa atacagcaat gaattccttg
     1561 ccatcggcgt gggcgcgcgt tcgctcgcca tgtcgggcgc ttccgtggcc agcgcggacg
     1621 acgtcacatc gggctactgg aacccggccg ggctgctggg cgtgaaaccc gatttccagg
     1681 tgggactcca gcacgcggaa tatttcgctt cgatcgcgaa atacgattac ctcggcttcg
     1741 ccggtaagat cgacagcgcc agctctttcg ggttcagcgt gctccgcttt gccgtggacg
     1801 acatcccgaa taccacgcag ctcatcgatc cttcgggcaa catcaactac gacaacatca
     1861 ccactttctc cgcgcaggat tatgcgttcc tcatctcgta tgcgcgcaag gcgcccatcg
     1921 cgggactgca ttacggcgcc aacgtgaaga tcatccgccg caaggtgggc gatttcgcag
     1981 gcgcgtgggg attcggtctc gatgcaggcg cgcagtacga ttacaaaggc tggcggctgg
     2041 gcgccgtggt ccgcgacgtg acgtcgacgt tcaatgcgtg gagcttcaac ctttcgcagg
     2101 acatgaaaga cgtgttcgcc gcaacgggaa atgagatccc gcagaattcg ctcgagctga
     2161 ccatgccgcg gcttattttc ggcgcttcga aaacttatcc tgtaggcagg cagcagaaat
     2221 tttccgtcgg cggtgaaatg gatttcatca atacgttcga cggcaaacgc aacgtgcttg
     2281 tgcgcaccgg gctctggagc atggaacctg ttcttggtct cgaagccggc ttcaaaaaca
     2341 tcatcttcct ccgcaccggt atcgggaaca tccagaaatc gaaagacgtt ttcggaaaag
     2401 acgtgacgac gttccagccc aatctcggtg tgggtctccg catcaaatcg ctgacgatcg
     2461 attatgcgtt caccgatatc ggcaaccagt cggtcgcatt gtattcgaat attttctccc
     2521 tgaagatcga tatcaacaag aaaaaaatcc cgggaggaag ctgagaaacc tgtttcagcc
     2581 gacccaccac ccctgccgat gaaacaattt taccggaaag cttttttctc ttcgacagga
     2641 atattttttt tcctcttctt cctgttcccg cctgacgttt catcgcagac ttacaacaat
     2701 tcctggatca gctattcgca gcactacctg aaaataaagg tggtgaacga cggcgtgtac
     2761 cgcatcgatt caaccgtgct ttccggtgca atgaattcca tgggcatccc cctttcttcg
     2821 gttgatccgc gcaatttcca ggtcttccat aacgggcagg aacagtacat ctgggtgcag
     2881 ggcgaaagcg acggcgtatt caacgccggc gattacatcg aatttctcgg ctaccgcaac
     2941 gacggacgtc ccgattcttc gctgtacgct tctcccgatt ccctgctcga tccgtattac
     3001 agtctttaca acgacacttc cgtttatttc ctcacctgga ataatctttt tacgaacctg
     3061 cgcatgaccg cttccaatga cacggccttc agcgcttaca caccggcgga ctatttcacg
     3121 cacagcgaga tcatgcgcgg caccggaacc tacatgcccg gcgtggcgga tgcgctcggc
     3181 atcaccgacc ccgctttcat tccctcggaa ggttattaca ccggcgtttc ctattacgcg
     3241 caacccgtga cgtacaatct ttcttcggcc aatgcgtaca ccggtccggg tgcgccgaac
     3301 gcgcagctga gcatgaaagt gggaacgttc tccaacgact ggaatgtcgc gaacgacaac
     3361 gatgtgcgca tacagtatcc cggcggacct gtgttcgatt ccatctacga cgggttcgcc
     3421 gttggatcgt atcatttcgc ggttgccgcc gcttcgctgg gcgccgccgc gccggtcact
     3481 gtcacttctg tcaactcaaa tggttcatcc tcttccgggc ttatcggaac ggcgttcatc
     3541 aatcttacat acccgcatac gttcgacatg gaatcgcgta ccggcatgta cggcacgttg
     3601 ccggataaca ccaccggtcc gaaaacgctc gtgaagtttt ccaacctcag cggtgcagcg
     3661 cccgcgcgtg tttacgatct ctacaatcac cagcgcatcg acgccgtgca gaacgggatc
     3721 tattattacg cgctcgtgca gaacgggaac ggctctgaga aaccggtgct tttctcttcc
     3781 gaagcgaacg tcgtgaacgt agcatccgtt cagccggaga acggcagcgg cctgttcacc
     3841 gatttcagcc agcaggccgc cgactccgtt tttattatca tcacgcaccg ttcgctgatg
     3901 ggcgtgggaa cgcagtacgc agcgtaccgc tcgtccattg caggaggttc gcacaacgta
     3961 gtgctcgctg atgtgaacga gctgtacgac cagttcgcgt ggggcatcgg tgaagatccg
     4021 ctcgcgatcc ggaacttctg cgctttcatg atcggaacct tcccgacacc gccacaggac
     4081 ctgctgctgc tcgggaaatc gtattatgcc gattacgcaa ggctttatgg ttattacggc
     4141 gtcaaccttg ttccgacgat gggctatccg ccctgcgata attcgctcac cgcggggctg
     4201 aacggaacac tgtgggaacc cgccattcct accggcagga tcgccgctgc cgattccgcg
     4261 caggcgcaat ggtacctcaa caaagtgatc gaatacgaga acaaccagcc ggccgaatgg
     4321 atgaaatacg tgctgcactt cgggggagga acgtccacat cggaacagca ggcctacaaa
     4381 agctatctcc agaattacca gcacacgatc gaggacacgc tgttcggcgg atacgtgcag
     4441 acgtacctga aaaattcttc cgcaccgatc cagatcaacc agtcggattc tttacgccag
     4501 cgcatcgagg acggcgtttc catcatgaca ttcttcggtc acgcgagcgg aaccggtttc
     4561 gaccagagca tcgatgatcc gtcgacgtac aacaacttcg accgttaccc gttcctcatc
     4621 gcgaattcct gctacgcagg cgacattcat tccgacggca tttcttccag cgaagcgttc
     4681 acgctcatcg accagaaagg aacaatcggc tacctcgctt ccgtcggcgt gggcattccg
     4741 caatacctcg acatgtacac ctcgcggttg tataaatcgg tcgggcaggt gaattacgga
     4801 caaccggtgg gcaaatgcat ccagtggacc gtgcacaacg cggaaacgac gcagcagcaa
     4861 agcatttatc tcaagaccac ctgcatggag atgacgctgc agggagatcc ggccatcgtc
     4921 atcaattcgt tc
//
LOCUS       DASTSF010000129         9921 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_366137,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000129 DASTSF010000000
VERSION     DASTSF010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 9921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9921
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_366137"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1231
                     /locus_tag="VFU15_03780"
     CDS             <1..1231
                     /locus_tag="VFU15_03780"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU4716921.1"
                     /translation="WWKSTPRVPKPLFSRMPASSLHILFLVSWYPNRNAPVLGNFIRR
                     HAEAAALRHKISVVYACSGENVAEGKTEVQLSSAGNLTECIVYYGKVRSTTPVIAQVK
                     KRSVYREAMKEGIRLVREKNGRADILHVHVIWPAVHAALPLLEETDIPLVISEHWSGY
                     LPEDGNYRGVLLKFFSTKLAEKARHITVVSSRMKDAMLAHGFRNSFSILPNCADETVF
                     FPAEKKENGKIKLLHVSMLVDREKNVSGMLRAMKRFEKNHDISLHIIGDGPERQTHEE
                     TARRLSLLQKNVFFHGLKNSAEIAQEMRTSDALLLFSHFEGMPVTIIEAQLCGLPVIA
                     TKTGAIPDMVRSTEGMLVDPADEEQLFNAILSFAAKKNDYDRAAISRRAMENYSVDAV
                     SRHLDSIYQNVMTHGGA"
     gene            1218..2882
                     /locus_tag="VFU15_03785"
     CDS             1218..2882
                     /locus_tag="VFU15_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716922.1"
                     /translation="MAEHKEIRVAVLCRGYVFDSWEAECIREVQSLPFVKIVLLVQEN
                     YPEKKISLSKKIIDYPYRRLKWRVYKRFFLRVPAMKPASIQKELDGIPVVKCTPELKG
                     KFSEHFPGEVIAKIKEQRCDVVLRFGFNILRGEILSATRYGVWSFHHADEQVIRGGPG
                     GFWEIYKGIPATGAILQRLTEKLDAGIILRKGNFRTIFHSYRDNLDQLLRGTTSWMKQ
                     ALIDAANGVSPAENGTPVKTNAPVFTYPRNLQMRIFRRRLLAGKIRFHFRALFRAETW
                     TIGYVEQEASSLLEENPFGNVHWFPEAGKGKYLADPFGRINEKKELVIYAEEYDYAKK
                     KGIICRSEGKTFSPFLEKKEHLSYPFLVNDNGTDFLVPECFESGKIFCVNAQNATEER
                     ILLGNFPAVDATPVKWNGRWWIFCTKEGEFSNTELYVFHSENFAGPWQPHANNPVKTD
                     ILSARPAGTPFVVNGRLYRPSQVCAPQYGVAVKINEVTKLSPIEFSEIAVREHAPPAG
                     WKFSKGLHTVSVAGDRTILIDAKRYAFNFDNFRRVLKTKLKRLFRK"
     gene            2879..4150
                     /locus_tag="VFU15_03790"
     CDS             2879..4150
                     /locus_tag="VFU15_03790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716923.1"
                     /translation="MIRKILSNFSARMAGAVLNFFMLLLTTHFLGKSVRGEIVLIQIG
                     INIIHLVSDLAGGPSLVYLVPRARLSRLLSVGSAWGIGSSVLVAFAMISYGAMPENYT
                     WQILVMGALISLHSLNQNILLGQERITAYNALLFAQGIVQTLVMTVCIIGLGQRGALP
                     YIWCCIIGYGLCWITGLFLVWKGHREPKIEEKRNIFLLLFSNGFFTQMASLAMLMSIR
                     INYYMLHKYMPDGDSSVGLYSTAISLGEAILLFSASISAIIMSRVSNESDHRTARSTV
                     LRLSKVSLGMTALGVCAFLVLPDGFYSWLLGKDFSPVKEIFTTIAPGVIILSFATVFS
                     HYFSGSGLHYLNFISGAVAILCTFIVAGPLISDYGIPGAGWAASISYGTLSLTVFFIF
                     MLVGGNRKEEWKMLLPSSADFSLKNYRQNNF"
     gene            4152..6143
                     /gene="asnB"
                     /locus_tag="VFU15_03795"
     CDS             4152..6143
                     /gene="asnB"
                     /locus_tag="VFU15_03795"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HEU4716924.1"
                     /translation="MCGISGIAGFSSQQDILEAAVKMNAAIRHRGPDGEGFLVVSENE
                     TVSCFSSDTPAEIISSSFPHSPKKNISEQKNAAKIVLAHRRLAIIDTSPAGHQPLCSA
                     DGNTWITYNGEVYNYIELRTELEQHGYTFRTRTDTEVILAAYDKWGKSCCEKFNGMWA
                     FVIWDSRKKMLFGSRDRFGVKPFYYYHDKNVFTFASEQKALLKNPFVQTSLSEVAVAD
                     YFVAGEIEYREESFFRNILELFPGCAFTLGTESGAFTQWQWFSPEEQEEEANYTEAQY
                     AKFIGETRELLTEAVRLRMRSDVPVGSCLSGGIDSSAISGIIGDMISRHEKINVGERL
                     KLFTAVFDDAEIDERKWAAEMVSRTGAEWFTVTPEPGELVRDIEELIFSQDVPIWSTS
                     TYAQHRVMKLVRDNGIRVVLDGQGGDELFAGYKPYFIPFWNELRSAGQTGILAAEKNA
                     FGLHEAKLYNRRERLKQVTFPSLPLSLRLLVQKNYFPDIAYLDKALISVYKKNYRPVS
                     PPKTLNEALRGEFVNTRLKGYLKCEDRCSMWHSVESRTPFADDHKLIGRVFRIPGTMK
                     IRNATTKYLLREAAAPFIPDSIRNRKDKLGYATPNNKWITQLRDQFRPYFEQDFGGIL
                     KKEKLLKDFDTFFSVEGMPENGRTFRFIAFAVWKKVTGL"
     gene            6212..6544
                     /locus_tag="VFU15_03800"
     CDS             6212..6544
                     /locus_tag="VFU15_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013455577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEU4716925.1"
                     /translation="MLDGFKRIFGLGGSMLPDELLLEKNKIIVDVRSQSEFNAGHIKG
                     SINIPVGAIEHNIGKLKGKSPIILCCASGARSGMATSILKGKGFKEVYNAGGWAGLNE
                     QLQSLKEK"
     gene            6544..6843
                     /gene="trxA"
                     /locus_tag="VFU15_03805"
     CDS             6544..6843
                     /gene="trxA"
                     /locus_tag="VFU15_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEU4716926.1"
                     /translation="MAASFRELINSGKPVLVDFFAEWCGPCKAMAPVLSEVSSRVGDS
                     AHILKVDVDKNPAAAGSYGIRAVPTMLLFKNGNVVWKHSGTMGPDELERVIRSAM"
     gene            6913..7638
                     /locus_tag="VFU15_03810"
     CDS             6913..7638
                     /locus_tag="VFU15_03810"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEU4716927.1"
                     /translation="MKKFIFLFPVFAFLVSCTSSSSMAGGSVPAGRFSVLIDSLAGVQ
                     LIDVRTPGEFNSGHISGAQNIDWNGSGFSDGAMALDKSKPVLVYCLSGGRSHEAAEFF
                     RKNGFKTVYELENGLRSWSMANLPLTQETGQQSTASPAFTTGDFSKTVNSGLLVLVDF
                     SATWCRPCQMLKPTIETIEAERKGSLTVLKLDADRDSQLADSLRITALPTLMIFNNGK
                     MTWRNEGLVPKDLIDSKLDEAKK"
     gene            complement(7678..8127)
                     /locus_tag="VFU15_03815"
     CDS             complement(7678..8127)
                     /locus_tag="VFU15_03815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716928.1"
                     /translation="MKSLKTLAAGGFLFVLTASFVLTGDTSSIEGKVYKVSISEAKKG
                     KSGPNSDDDVSFKSGKFKCKFFGKNAGADAIAIELTTDSTYSDDSGEDMLYVEFEGEM
                     TNKLEENVKVTGTIDGYGIEGNVELSKKGKVKHHWDYVGSLKEKKKH"
     gene            8294..9568
                     /locus_tag="VFU15_03820"
     CDS             8294..9568
                     /locus_tag="VFU15_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017257189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEU4716929.1"
                     /translation="MSIPLAERLRPATLDEYIGQKHLVGEGAVLRKAIESGNIHSMIF
                     WGPPGVGKTTLANIIASRLKRPFYALSAINSGVKDIREVIDKAKSQAFFGTNQPVLFI
                     DEIHRFSKAQQDSLLGAVEKGTITLIGATTENPSFEVISALLSRCQVYILKPLEKDDL
                     VQLLHHAAEKDPELKKKKIVFEEYEALLRVSGGDARKLLNALELVVNAANADEITITN
                     ESVLKTIQQNLALYDKSGEQHYDIISAFIKSMRGSDPNAAVYWMARMIAGGEDPLFIA
                     RRMVILASEDIGNANPNALLLATSCFQAVNMIGMPESRIILSQCATYLASSPKSNASY
                     MAIETASALAASTGDLPVPLHIRNAPTSLMKEIGYGKDYEYAHAHEGNFSEQEYLPDR
                     IKGSKIYDPGNNARENELRAYLKKCWKEKYGY"
     gene            complement(9562..>9921)
                     /locus_tag="VFU15_03825"
     CDS             complement(9562..>9921)
                     /locus_tag="VFU15_03825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716930.1"
                     /translation="FELLPYSLSIYLSAAAVVYLLIGFLLSRAFISPFFDKIARHPVL
                     RGPAVGFGVGVMVYLVAMVVHVTFNKDLDFRYLLLDITWQGVEEALGGLVVGIVYLVV
                     FENVFHPVPEENDHEDQ"
BASE COUNT         2390 a         2754 c         2585 g         2192 t
ORIGIN      
        1 atggtggaaa tctacgccac gcgtgcctaa acctttattt tcgcgtatgc ccgccagttc
       61 cctgcacatc ctgtttctcg tttcctggta tcccaaccgg aacgcgccgg tcctcgggaa
      121 ttttatccgc cgtcacgcgg aggctgccgc actgcgacac aaaatctctg tcgtttacgc
      181 ctgctccggc gaaaatgttg cggaaggaaa aacagaagtg cagctttcct cagctggaaa
      241 tcttacggaa tgcatcgtgt attacggaaa ggttcgttca accacaccgg tgatcgcgca
      301 ggtaaaaaaa cgttctgtat accgtgaagc gatgaaagaa ggcatacggc tcgtgcgtga
      361 aaagaacggc cgtgcggata tactccacgt gcatgtcatc tggcccgcag ttcatgcagc
      421 gcttccgctg ctggaagaaa cggatatccc gctcgtcatc tcggagcatt ggtcgggtta
      481 ccttccggag gacgggaact accgcggcgt cctgctgaaa ttcttctcca cgaagctggc
      541 ggaaaaagca agacacatca ccgttgtttc atcacggatg aaagacgcca tgctcgcgca
      601 tggattccgc aattcatttt ccattcttcc caactgcgcc gatgaaacgg tattcttccc
      661 cgctgaaaaa aaagagaacg gaaaaataaa gctgctgcac gtttccatgc tcgtcgaccg
      721 ggaaaaaaat gtttccggga tgctgcgcgc catgaagcgc ttcgaaaaga accacgacat
      781 ctccctgcac atcatcggcg acgggccgga acgacaaacg catgaagaaa ccgcacgccg
      841 gctttcgctg cttcaaaaaa atgttttttt tcacggcctg aaaaactcag cggagatcgc
      901 gcaggaaatg cgaacatccg atgcgctgct gctgttcagt cattttgaag gcatgcccgt
      961 gacgatcatc gaagcgcagc tgtgcggact tcccgtcatt gccacaaaaa ccggcgccat
     1021 cccggacatg gtccgcagca ccgaaggaat gctcgtcgat cccgcagatg aagagcagct
     1081 tttcaacgcg atcctttctt tcgctgcgaa aaaaaacgac tacgaccggg ccgcgatcag
     1141 ccggcgcgcc atggagaatt acagcgtgga tgcggtttcc cgccatctcg attccattta
     1201 ccagaacgtg atgacgcatg gcggagcata aggaaatacg cgtcgctgtt ctttgccgcg
     1261 ggtacgtttt cgattcctgg gaggcagaat gcatccggga agtgcagtcg ttgccgttcg
     1321 tgaagatcgt tctgctggtg caggaaaatt acccggagaa aaaaataagc ttgtcaaaaa
     1381 agatcattga ttacccgtac cggcggttaa aatggagggt ctacaaacga tttttcctgc
     1441 gggtcccggc catgaaaccg gcgtcgatcc agaaagagct ggacggaatt cctgtcgtga
     1501 aatgcacgcc tgagctgaaa ggaaaattct ccgagcattt tcccggcgag gtgatcgcaa
     1561 aaataaaaga gcagcgctgt gatgtcgttc tgcgcttcgg gttcaacatc ctgcgcggcg
     1621 aaattctgtc ggccacccgg tacggcgtat ggtcgttcca tcatgccgac gaacaggtga
     1681 tccgcggcgg acctggcggc ttctgggaaa tttacaaagg cattcccgcc acgggcgcca
     1741 tcctgcagcg tctcacggaa aaactcgacg cgggcatcat cctgcgcaag ggaaatttcc
     1801 ggacgatctt tcattcgtac cgcgataatc tcgaccagtt gctgcgcgga acgacttcct
     1861 ggatgaagca ggcgctgatc gatgcagcga acggcgtttc gcccgcggag aacggaacac
     1921 ctgtgaaaac aaacgctccc gtattcactt acccgcgcaa cctgcagatg cgcattttcc
     1981 ggcgccgcct cctggcagga aagatccgtt ttcatttccg ggcgcttttc cgcgcagaaa
     2041 cgtggaccat cggttatgtg gagcaggaag cgtcgtcgct gctggaagaa aacccgttcg
     2101 ggaacgtgca ttggttcccg gaagccggga aaggaaaata cctggccgat ccgttcgggc
     2161 gcatcaatga aaagaaagag ctggtgattt atgcggaaga atacgattac gcgaagaaaa
     2221 aagggatcat ctgccgcagc gaaggaaaaa cgttttctcc tttccttgaa aaaaaagagc
     2281 acctctccta cccgtttctt gttaatgaca acggaacgga ttttctcgtt cccgaatgtt
     2341 tcgaatcggg aaagatcttc tgcgtgaatg cgcaaaatgc gacggaagaa agaatacttc
     2401 tcggaaattt tccggccgtc gatgcaaccc cggtgaaatg gaacgggcgc tggtggatct
     2461 tctgcacgaa agaaggagaa ttcagcaaca cggaactgta tgtttttcac tcggaaaatt
     2521 tcgccggtcc ctggcagccg cacgcgaaca acccggtgaa gacggacatt ctttccgcgc
     2581 ggcctgccgg aacaccgttt gttgtgaatg gaaggcttta ccgtccttca caggtatgcg
     2641 ccccgcaata tggcgttgcc gtgaagatca acgaagtgac caaactttca ccgatcgaat
     2701 tttccgagat cgccgtaagg gaacacgccc ctcccgccgg gtggaaattc agcaaaggat
     2761 tgcacaccgt ttcagtcgca ggcgaccgca caattctcat cgacgcgaag cgttacgcat
     2821 ttaattttga taatttcagg cgggttttga aaacgaagct gaaacgcctt ttccgtaaat
     2881 gatccgtaag atcctctcca acttttccgc ccggatggcc ggtgcagtgc tgaatttttt
     2941 catgctgctg ctcaccacgc attttctcgg caaaagcgtc cgcggagaga tcgtgctgat
     3001 ccagatcggc atcaacatca ttcacctcgt gagtgatctc gccggcggac cttcgctggt
     3061 ttacctcgtg ccgcgtgccc ggctttcccg tcttctttcc gtcgggagcg catgggggat
     3121 cggctcgtcc gttcttgtcg cctttgccat gatctcttat ggagcgatgc cggaaaatta
     3181 cacctggcag atcctcgtca tgggcgcgct catttcgctg cattcgctca accagaacat
     3241 cctgctcggg caggaacgca tcacggcgta caatgcgctg ctttttgcac agggaatcgt
     3301 ccagacgctg gtcatgacgg tgtgcattat cggcctcggg cagcgcggcg cattgcctta
     3361 tatctggtgc tgcatcatcg gctacgggct ttgctggatc acggggctgt tcctcgtctg
     3421 gaagggccac cgggaaccga agatcgaaga gaaaagaaac attttcctgc tgcttttctc
     3481 caatggtttt tttacgcaga tggccagtct cgccatgctg atgagcatcc gcatcaatta
     3541 ttacatgctg cacaaataca tgcccgacgg cgacagcagc gtcggacttt attccacggc
     3601 tatttcactt ggtgaggcga tccttctttt ttccgccagc atttccgcga tcatcatgtc
     3661 gcgcgtttca aatgaatcgg atcaccgcac ggcgagaagc acggttctcc gtctgagcaa
     3721 agtgagcctc ggcatgacgg cgctcggcgt ctgcgcattc cttgttcttc ccgatggatt
     3781 ctattcctgg ctgctgggaa aagatttttc cccggtcaag gaaattttca cgacgatcgc
     3841 gccgggtgtg atcatcctca gcttcgccac ggttttctcg cactattttt ccggctcagg
     3901 cctgcattac ctcaatttca tttccggcgc cgtcgccatc ctctgcacgt ttatcgtggc
     3961 cggtccgctg atctctgact atggaattcc cggcgcaggc tgggcagcat ccatctcgta
     4021 cggcacgctt tccctcaccg tctttttcat cttcatgctc gtcggcggga accggaaaga
     4081 ggaatggaaa atgctgctgc cttcttccgc ggatttttcg ttgaagaatt accgtcaaaa
     4141 taatttctga tatgtgcggg ataagcggga ttgcaggctt ttcatcgcag caggacatcc
     4201 tggaagcggc tgtgaaaatg aatgccgcca tccggcaccg cggtccggac ggagaaggct
     4261 tccttgtcgt ttcggaaaat gaaacggtca gctgtttttc ttccgacaca cccgctgaaa
     4321 ttatttcttc ctcttttccg cattcgccga aaaaaaatat ttcagaacaa aaaaacgcag
     4381 cgaagatcgt gcttgcacac cgtcgcctgg cgatcatcga tacgagtccc gccggccatc
     4441 agccgctctg ctctgccgat ggaaatacct ggatcacgta caacggcgaa gtttacaatt
     4501 acatcgagct gcgcaccgag ctggaacagc atggctacac cttccgcaca cgcaccgata
     4561 ccgaggtcat tctcgccgct tatgataaat ggggaaagag ctgctgcgaa aaattcaacg
     4621 gcatgtgggc gttcgttatc tgggacagcc ggaaaaaaat gctgttcgga tcacgcgacc
     4681 gcttcggggt gaaaccgttc tactactatc acgataaaaa cgtttttacg ttcgcttcgg
     4741 agcaaaaagc gctgctgaaa aacccgttcg tccaaacttc gctcagcgag gtcgcggtgg
     4801 cggattattt cgttgcgggc gagatcgaat accgcgagga aagctttttc cgcaacatcc
     4861 tcgagctctt tcccggctgc gcattcacgc tcggcacgga aagcggtgca ttcacccaat
     4921 ggcaatggtt cagcccggaa gaacaggaag aagaagcgaa ttacaccgaa gcgcagtacg
     4981 cgaaattcat tggagaaacg cgcgagctgc tgaccgaagc cgtccgcctg cgcatgcggt
     5041 cggatgttcc cgttggctcc tgcctcagcg gcgggatcga cagctcggcg atttccggga
     5101 tcatcggcga catgatcagc cgtcacgaaa aaataaacgt gggcgaacgg ctgaagctgt
     5161 tcaccgccgt cttcgacgat gcggagatcg acgagcggaa atgggcggcg gaaatggttt
     5221 cgcgcacggg cgcggaatgg tttacggtaa caccggaacc gggcgagctg gtccgcgata
     5281 tcgaagagct tattttcagc caggatgttc ccatctggag cacgagcact tatgcgcagc
     5341 accgcgtcat gaagctggtg cgcgataacg gcatacgcgt cgtcctcgac gggcagggcg
     5401 gcgatgaatt gttcgcgggc tacaaaccgt atttcatccc gttctggaac gagctgagat
     5461 cggcggggca aacaggcatt ctcgccgcgg aaaaaaatgc gttcggactg cacgaagcaa
     5521 agctttacaa ccggagggaa cgattgaaac aggtgacgtt cccttccctc ccgctcagcc
     5581 tgcgcctgct cgtgcagaaa aattatttcc ccgatatcgc ctatctcgac aaagcgctca
     5641 tttcggttta caaaaaaaat taccgtcccg tttctccgcc gaaaacactc aacgaagcgc
     5701 ttcgcggtga attcgtcaac acacggctca aaggttacct gaaatgcgag gaccgctgct
     5761 cgatgtggca ttccgtggaa tcgcgcacgc cgttcgccga cgaccacaaa ctgatcgggc
     5821 gagttttccg catcccggga acgatgaaga tccggaacgc cacaaccaaa tacctcctcc
     5881 gtgaagccgc ggctcccttc attcctgatt ccatccgcaa ccggaaagac aaactcggct
     5941 acgccacccc gaacaacaaa tggatcacac agctgcgcga ccagttccgg ccttacttcg
     6001 aacaggattt cggcggcata ctgaaaaaag aaaagctgct gaaagatttc gacactttct
     6061 tttccgtcga aggaatgccg gagaacggga ggaccttccg cttcatcgcg ttcgccgtct
     6121 ggaaaaaagt gacaggcctc tgagacttct gtgaccgggg tcacgttccc cgcctttttt
     6181 ccaccgcaac tttgcacaaa acaaaataat tatgctggac ggattcaaac gtattttcgg
     6241 tctcggtgga agcatgcttc ccgacgaact gctgctggaa aagaacaaga tcatcgtgga
     6301 cgtacgttca cagtcggagt tcaacgcagg acacatcaaa ggctccatca acattcccgt
     6361 gggagcgatc gaacacaaca tcgggaagct caaaggcaaa tcgccgatca tcctttgctg
     6421 cgcttccggc gcacgcagcg gcatggcgac ctccatcctg aaaggaaaag gattcaaaga
     6481 agtctataac gccggcgggt gggccgggct caacgaacag cttcaatctt taaaagaaaa
     6541 ataatggctg cttcgttcag agagctgatc aattcgggaa aaccggtgct cgtcgacttt
     6601 tttgctgagt ggtgcggacc gtgcaaggcg atggcaccgg ttctttccga agtctcttcc
     6661 cgcgtcgggg attccgcgca catcctgaaa gtggatgtgg ataaaaatcc tgcggccgcc
     6721 ggcagctacg gtatccgcgc cgttcccacc atgctgcttt tcaagaatgg aaacgtggta
     6781 tggaaacatt cgggaacaat gggcccggat gaactggaac gggtgatacg atccgcaatg
     6841 tgattgtcgt cccgaaccgc gaacgcgaaa ctttgagact ttccaaactt tcataaacag
     6901 tacttttgcc gcatgaaaaa attcatcttc ctcttccccg ttttcgcgtt cctggtttcc
     6961 tgcacctcct cttcttcgat ggcaggcgga agcgttccgg ccggccgttt ttcggtgctg
     7021 atcgattccc ttgccggcgt acagctgatc gacgtgcgca cgccgggcga attcaattcg
     7081 ggacacatca gcggtgcgca gaacatcgac tggaacggga gcggcttcag cgacggcgcg
     7141 atggcgctcg acaaatcgaa gcccgtgctg gtgtattgcc tttccggcgg aagaagccat
     7201 gaggcggcgg aatttttcag gaagaacggt ttcaaaacag tttacgagct ggagaacggg
     7261 ctgcgctcct ggtcaatggc caatctcccg ctgacgcagg aaaccgggca gcaatcgaca
     7321 gcctcaccgg ctttcacgac gggcgatttc agcaagaccg ttaattcggg tttgctcgtg
     7381 cttgtggatt tcagcgcaac gtggtgcagg ccctgccaga tgctcaagcc cacaatcgaa
     7441 acgatcgaag ccgaacgaaa aggttcgctc accgttctca aactggatgc cgaccgcgat
     7501 tcacagcttg ccgattcgct gcgcatcacc gcgcttccca cactcatgat ctttaacaac
     7561 ggtaaaatga cctggaggaa cgaagggctt gttcccaagg acctcatcga ctcgaagctc
     7621 gacgaagcga agaaataaaa aagcgccgcg gtttcgcggc gcctgctttt attgtgatca
     7681 gtgctttttc ttctctttca ggcttcccac gtaatcccag tggtgtttca ccttgccttt
     7741 cttcgaaagc tcgacgttgc cttctattcc gtagccgtcg atggtgccgg taacttttac
     7801 attctcctcg agcttgttcg tcatttctcc ttcgaattcg acatacagca tatcttctcc
     7861 cgaatcatcg ctgtaggtcg agtcggtcgt cagctcgatc gcgatcgcat ctgcgcctgc
     7921 attcttcccg aagaatttgc atttgaattt tccgctcttg aacgaaacat cgtcatcgct
     7981 gttgggcccg gattttcctt tcttcgcttc cgaaatgctg accttgtaaa cttttccttc
     8041 gatcgacgag gtatcgccgg tgagcacgaa cgaggcggtg agcacgaaca ggaatcctcc
     8101 tgcagcaagg gtcttcaggc ttttcatctt tttctttttt tgacgttcac aaagttaaac
     8161 tttaagggat acgtccgtaa aaaccgtgcc gtttcacagg ccggcagcat gaattcctat
     8221 acggaagcga aaagccggag aacttcggac attgaacgtt gaacattaaa ccttaaaaac
     8281 cgacctttac ttcatgagca ttccactggc cgaacgtctg cgacccgcca cgctcgatga
     8341 atacatcggg cagaaacacc tcgtgggaga aggtgctgtc ctgcgcaaag cgatcgagtc
     8401 cgggaacatc cattcgatga tcttctgggg tccgccgggc gtgggaaaaa cgacgctcgc
     8461 gaatatcatc gcttcccggc tgaaacgtcc gttctacgcg ttgagcgcaa tcaactccgg
     8521 cgtaaaagac atccgcgaag tgatcgacaa agcgaaaagc caggccttct tcgggacgaa
     8581 ccagcccgtg ctgttcatcg atgaaattca ccggttcagc aaagcacagc aggattccct
     8641 gctcggcgcc gtggaaaaag gcacgatcac gctcatcggc gcaacgacgg aaaacccgtc
     8701 tttcgaagtg atatcggcac tgctttcccg ctgccaggtg tatatcctga aaccgctcga
     8761 gaaggatgat ctcgtgcagc tgctgcatca tgccgcggaa aaagacccgg agctgaaaaa
     8821 gaaaaagatc gtgttcgaag aatatgaagc tttgcttcgc gtttccggcg gagatgcgcg
     8881 caagctgctc aatgcactcg aactcgtggt gaacgcggcg aatgcggacg agatcaccat
     8941 caccaatgaa tcggtgctga aaacgataca gcagaacctt gcgctgtatg acaaaagcgg
     9001 cgagcagcat tacgacatta tttccgcgtt catcaaatcc atgcgcggca gcgatccgaa
     9061 tgccgcggtt tactggatgg cacgcatgat cgccggcgga gaagatccgc tcttcattgc
     9121 gcggagaatg gtcatcctcg cctccgaaga tatcggcaac gccaacccga atgcgctgct
     9181 cctcgccacc agctgtttcc aggcggtgaa catgatcggc atgcccgaat cgaggatcat
     9241 cctttcgcag tgcgccactt atctcgcttc ttcacccaag agcaacgcat cgtacatggc
     9301 catcgaaaca gcatcggcgc tcgcggccag caccggcgat cttcccgttc cgcttcacat
     9361 ccgcaatgcg cccaccagcc tgatgaaaga gatcggttac gggaaagatt acgaatacgc
     9421 gcatgcacac gaaggaaatt tcagcgaaca ggaatatctt cccgaccgca tcaaaggctc
     9481 gaagatctat gaccccggca acaatgcacg tgagaatgag ctgcgtgcat acctgaaaaa
     9541 gtgctggaaa gagaaatacg gttactgatc ctcgtgatcg ttctcttcag gaacggggtg
     9601 aaaaacattc tcgaaaacaa cgaggtaaac gatcccgaca acaagtccgc ccagcgcttc
     9661 ttccacaccc tgccaggtaa tgtcgagcag gagatacctg aaatcaaggt ctttgttgaa
     9721 ggttacgtgc accaccatcg ccacgaggta aaccatgacg ccgacgccga acccgacagc
     9781 aggtccgcga aggacgggat gcctggcgat cttgtcgaaa aaaggcgaga taaacgcacg
     9841 gctgagcagg aagccgatca gcaggtaaac gacagccgcc gcgctcaggt aaatgctcag
     9901 cgaatacgga agaagctcga a
//
LOCUS       DASTSF010000130         4185 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_142550,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000130 DASTSF010000000
VERSION     DASTSF010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4185
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142550"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2028)
                     /locus_tag="VFU15_03830"
     CDS             complement(<1..2028)
                     /locus_tag="VFU15_03830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716931.1"
                     /translation="MKNIYALSVFFLIAAGFGNHLSAQNPTDLCSGAPSLSVNASCVN
                     TAYTQAATHTVTVPNPSCATNYRDSWYKFTATTTSTTITASTNRDLAIVVYSGTCASL
                     TEIACANAAGFGGSESTTFGTAIGTTYYVALMRVNNAASNTMTGNICIVGSTPPANDD
                     PCSATALTVGGSCSYTAGNNNGASNSTGIPAPGCASYSGQDVWYTCTVPASGFLNITL
                     ETGTITDGGAALYTGTCGSLTLFSCDDDGAGSSGLMPYFNLSCVTPGTQIWIRVWQFG
                     GGTGTFQICAYNPGGSCTPPANDDPCSATALTVAASCSYATYTNQNAGPSVGVPAPGC
                     AGYSGGDVWFTATVPASGNLNIDTQTGVVLDGGMAAYSGTCGSLTLISCDDDASANGA
                     MPYLSLTCLTPGTTIWIRFWEYGNDNDGTFGICVTDPGASGSTPANDLPCNATSLSLS
                     VTTTGNNTNATNCNEPADPGCWVNGTNQSNTVWFSVVCPASGQLDIRTGLGTLTNTQI
                     EVFSGTCSSLTSIGCSNNVTLCGNTQNWSEVDLTGLTPGATYYVRVDGHNGQVGDFSI
                     TAIDGTSSWPPVFGQDCQQAMPVCSSTSTIGDPGFIGSGNYCDYPGNATGCPGGSCIY
                     VGERNSVWYSFTIGSNGTLSFTLTPNTPCDYDFALYDVSGNPNGCTDIASNS"
     gene            complement(2044..2439)
                     /locus_tag="VFU15_03835"
     CDS             complement(2044..2439)
                     /locus_tag="VFU15_03835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716932.1"
                     /translation="MKSTRVKILRASLFALLLLFTVKAKSQVISSPPPESRYTTLKIY
                     IGSYPDSMTSVRFSKSLVNSYDPKIVATAASFSEGTVTVTFDPSLLTEENLLQIIRMD
                     GYYNPCYFDGYTKYLLDAHGNLVSEDVKH"
     gene            complement(2701..3435)
                     /locus_tag="VFU15_03840"
     CDS             complement(2701..3435)
                     /locus_tag="VFU15_03840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HEU4716933.1"
                     /translation="MKILSHQLLLIPAFLFAGKVMAQQPATNDYDPKAKTILDDVSKT
                     AKSYSTITAEFTITTKKTDGTSDSKDGTIVLKGSKYKLTLNTKVKDKMYAEEYYNDGK
                     TTWAYVQKDNEVTIDNAPDPNKKKEGTFSVSDIFSDPAKGFKYRFIKEETLNGKVVQQ
                     IELYPEKPDKKNYHTIKLTIDKVKKQVISAVQVNNDQSTITYTIKKFTPNLDVPDSTF
                     QFDKKTHPGVKAIDLREDDDDGDSAK"
     gene            complement(3570..>4185)
                     /locus_tag="VFU15_03845"
     CDS             complement(3570..>4185)
                     /locus_tag="VFU15_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008504826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEU4716934.1"
                     /translation="IARLAQLARAIGIHLIIATQRPSVNIITGTIKANFPSRIAFRVT
                     SKIDSRTILDAGGAEQLIGRGDMLYSTGNDLIRLQCAFVDTPEVEKITDFIGNQRGYV
                     SAFLLPEYVDENSEAKLEIDAGDRDPLFEEAARIIVTHQQGSASLLQRKLKLGYNRAG
                     RIIDQLEAAGIIGPFEGSKARDVLVKDPMALEEILSSAGGKNEV"
BASE COUNT          898 a         1012 c         1202 g         1073 t
ORIGIN      
        1 ggagttcgac gcaatatccg tgcatccgtt gggattaccg gaaacgtcgt acaacgcgaa
       61 gtcatagtcg cacggtgtgt tcggtgtcag tgtaaagctg agcgttccgt tcgatccgat
      121 ggtgaaggaa taccagacgg aattccgttc cccgacgtag atgcaggaac cgccggggca
      181 cccggtcgca tttcccgggt aatcacaata gttcccgctg ccgatgaagc cggggtcacc
      241 gatggttgaa gtggagctgc acaccggcat cgcctgctgg caatcctgtc cgaaaacagg
      301 aggccagctg gatgttccgt caatggcggt gatgctgaaa tcgccgacct gcccgttgtg
      361 tccgtctacg cggacgtagt aagttgcccc gggggtgagg ccggtaaggt ccacttccga
      421 ccagttctgt gtatttccgc aaagcgtgac gttgttgctg cacccgatcg aggtaagcga
      481 actgcacgtc cccgaaaaaa cttcgatctg cgtattcgta agcgttccga gcccggtgcg
      541 gatatcgagt tggccggatg ccggacaaac cacggagaac catacggtat tagactggtt
      601 ggtaccattc acccaacagc ccggatcagc aggctcgttg caattcgttg cgttggtatt
      661 gtttcccgtc gtagtaacgc tgagtgaaag agatgtggca ttacagggaa gatcattggc
      721 aggcgtggac ccggaagcgc ccggatccgt aacgcagata ccgaacgttc cgtcattgtc
      781 gttcccgtat tcccagaaac gtatccagat cgttgttccc ggtgtcaggc aggtcagcga
      841 gagatagggc attgcgccat ttgcagacgc gtcgtcatcg cacgaaataa gtgtcagcga
      901 tccgcaggtt cctgagtagg ccgccattcc tccgtcgagg acgacaccgg tttgcgtatc
      961 gatgttcagg ttgccggaag cgggaacagt tgcggtaaac cacacgtcgc cgccggaata
     1021 acctgcgcat ccgggagcag gaacaccaac ggacggacca gcattctggt tcgtgtaggt
     1081 tgcgtagctg caggaagcgg cgacggtgag ggccgttgcg gagcagggat catcattcgc
     1141 aggaggcgta caggatccgc cgggattata cgcgcagatc tggaatgttc cggtgcctcc
     1201 gccgaattgc cagacgcgta tccagatctg ggttcccggt gttacacaac taaggttgaa
     1261 ataaggcatc agtccgctcg agccggctcc atcgtcatcg cagctgaaga gagtaagtga
     1321 gccgcaggta ccggtataaa gcgcggctcc gccatccgtg atggtaccgg tttccaaagt
     1381 gatattgagg aaacctgatg cgggtaccgt acaggtgtac catacatcct ggccgctgta
     1441 cgatgcacag ccgggtgcag gaattccggt cgagttggat gccccgttat tgtttcccgc
     1501 tgtatacgaa catgaaccac cgacggtaag cgcggtcgcg gaacagggat cgtcattggc
     1561 gggcggtgtg ctcccgacga tgcagatatt acctgtcatt gtatttgacg ccgcattatt
     1621 tacgcgcatc agggccacat agtaagttgt tccgatcgcg gttccgaacg tggtgctttc
     1681 cgatccgccg aaaccggcgg cattggcgca ggcgatctcc gtaagcgaag cgcaggttcc
     1741 ggaatacacc acaatggcga ggtccctgtt cgtgcttgcc gtgatcgtgg tggaagtggt
     1801 ggtcgccgtg aatttatacc agctgtcgcg gtagtttgtt gcacagctgg ggttgggtac
     1861 ggtgaccgtg tgagtggccg cctgtgtata cgccgtattc acacagctcg cattgacgct
     1921 gagcgagggc gcgccggaac aaaggtcggt agggttctgt gccgaaagat gatttccgaa
     1981 acctgccgca atgaggaaga aaactgaaag agcgtaaata tttttcatag ggtctgacag
     2041 ggattaatgt tttacgtctt ccgaaacaag attgccatgc gcatcaagaa gatacttggt
     2101 gtagccatcg aaatagcagg gattataata cccgtccatg cgaatgatct gcagcaggtt
     2161 ttcctccgtg agcagcgaag gatcaaatgt tacggttacc gttccttccg aaaaagaagc
     2221 tgcagttgct acgatcttgg gatcatagct gttcaccagg cttttggaga aacgtacgga
     2281 agtcatggaa tcgggataac ttccgatgta aattttaagt gttgtgtacc tcgattccgg
     2341 gggcggactg gagatcacct ggctctttgc ttttacagtg aaaagcagca gcagtgcaaa
     2401 caaacttgca cgcaggattt ttacccgggt tgatttcatg cgttaaaacg aattaggttg
     2461 tttgtgtttg ttaagtttgc ctttttcaaa tataaaaatc taattagtaa aatcaaaatc
     2521 agttagttac aaagcactaa cagcttccgg ggaaaacaaa agatgatgta tttggtcggg
     2581 gatttgacct ttccggcggc aaaaaagaag gttctcgtct aaattattgc agttaacctc
     2641 aattttcaga aaacaaaaaa gcggcttaac atcaagccgc ttttctgttc agaaagttta
     2701 ttacttggcg ctgtcgccgt cgtcgtcatc ttcgcgcaga tcgatcgctt ttacaccggg
     2761 atgtgttttt ttgtcgaact ggaacgtgga atccggaacg tcgagattgg gcgtgaattt
     2821 tttgatcgtg tacgtaatcg tcgactggtc gttgttcacc tggacggccg agatcacctg
     2881 cttctttact ttatcgatgg tgagcttgat ggtgtgataa tttttcttgt ccggcttttc
     2941 ggggtagagc tcgatctgct gcaccacttt cccgttgagg gtttcttcct tgatgaagcg
     3001 gtatttgaat cctttcgcgg gatcggaaaa aatatcgctt acggaaaacg ttccttcttt
     3061 tttcttgttg ggatccggtg cgttgtcgat cgtcacttca ttgtcctttt gcacatacgc
     3121 ccaggtggtt ttgccgtcgt tgtaatattc ctccgcgtac attttgtctt tcaccttcgt
     3181 gttcagcgtc agcttgtact tgcttccctt gagaacgatc gtaccgtctt tggagtcgga
     3241 agtgccgtct gtttttttcg tggtaatggt gaactcagcg gtgatcgtgg aataggattt
     3301 cgctgttttg cttacgtcgt cgagaatggt tttcgctttc gggtcgtaat cgttcgtggc
     3361 gggttgctgc gccattactt tcccggcaaa aagaaatgca gggatgagca gcaattggtg
     3421 tgacaggatt ttcattgctt gattttttct ggaggagata attcaaaaag cgttccaaaa
     3481 ttacgaatcg cttttgttca gcgttttccg gccgggattt ttagtctttt ttttgtctga
     3541 cgaagctggt gtgcaatcgt ttaacgtact cacacctcat ttttgccgcc ggccgaagaa
     3601 agaatctcct cgagcgccat cggatctttc acgagcacgt cgcgcgcttt gcttccttcg
     3661 aacgggccga tgatgcccgc ggcttccagc tggtcgatga tgcgtcctgc gcggttgtaa
     3721 ccgagcttca gcttgcgctg cagcaatgat gcggatccct gctggtgcgt gacgatgatg
     3781 cgcgccgctt cttcgaagag cgggtcgcgg tcgccggcat cgatctcgag cttcgcttcg
     3841 ctgttctcgt ccacgtattc gggcagcagg aacgcactga cgtagccgcg ctggttgccg
     3901 atgaagtcgg tgatcttttc cacttcgggc gtatccacga acgcgcactg caggcggata
     3961 aggtcgttgc cggtggagta gagcatgtcg ccgcggccga tgagctgctc ggcgccgccc
     4021 gcatcgagga tcgtgcgtga atcgatcttc gacgttacgc ggaaagcgat gcgggaagga
     4081 aagttcgctt tgatcgttcc cgtgatgatg ttgaccgaag gccgctgcgt ggcgatgatg
     4141 agatggatgc cgatagcgcg cgccagctgg gcgagacgtg caatg
//
LOCUS       DASTSF010000131         2134 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1929568,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000131 DASTSF010000000
VERSION     DASTSF010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2134
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1929568"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1356)
                     /locus_tag="VFU15_03850"
     CDS             complement(<1..1356)
                     /locus_tag="VFU15_03850"
                     /inference="COORDINATES: protein motif:HMM:NF037626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HEU4716935.1"
                     /translation="MITEISGARAAGIQLRGLAGSAGAFFASAVLQRSSQSHLFILGD
                     KEEAAYFLNDLEGLLGVNMDETGTRSSFPVLFFPASYRRPYQVEDTDNANVLLRAEVL
                     DRIRREKKVAVVTYPGALAEKVVTRQLLEKNTLQLKRGEKISIAFVTDLLNEYGFERN
                     DYVYDAGQYAVRGGIVDVFSFANEHPYRIEFFGDDVESIRTFDPLTQLSVKNLDRLTI
                     IPNVQGKLLTESRESFFEYFFTARHDGQNVNVVWISDIETTGAVVQKEFEAAEKAFAE
                     RTEESPVRHLSPAEQFIDEATFRETLRRFHVIEYGSRFILENARAITFSTHPQPAFRK
                     NFDLLTQELAKNSAAGMKNIVFTDSAKQAERLHSIFTDIGRKLDYSPIVHSVHEGFSD
                     TDLKIACYTDHQVFERYHRFRLRDGFQRKKEAMTIKELRGLNPGDYVVHIDHGVGRFG
                     GL"
     gene            1659..>2134
                     /locus_tag="VFU15_03855"
     CDS             1659..>2134
                     /locus_tag="VFU15_03855"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEU4716936.1"
                     /translation="MKRLALSFCLIGFSAVTLFAQTGKGWREVGGMGRLDLNYKNKAY
                     NFDLTPEMYWFTGDHLALGTDFGGGFAALKDSSFSSSNVYFYVAPGLRYFFGTPDKNK
                     WRPYLFGNGGYQFGATHTKFNGTSTNGTNNGFRGYAGTGVAWFFSDHAAFDIRMRIV"
BASE COUNT          481 a          591 c          555 g          507 t
ORIGIN      
        1 aagtccgccg aaacgtccca cgccatgatc gatgtgcacg acataatcgc ccgggttgag
       61 tccgcgcaat tctttgatcg tcatcgcttc cttcttccgc tggaaaccgt cgcgcaggcg
      121 gaagcggtga taacgttcga acacctggtg atccgtgtag cacgcgatct tcagatcggt
      181 atctgaaaaa ccttcatgca cagaatgcac gatgggcgaa taatcgagct tgcggcctat
      241 atcggtgaag atggaatgca ggcgttcggc ctgcttcgcg ctgtcggtaa agacgatgtt
      301 cttcattccg gcggcagaat ttttcgccag ctcctgcgtg agcaggtcga aatttttcct
      361 gaacgccggt tgcgggtgcg tgctgaacgt tatcgcgcgt gcattttcaa gaatgaaacg
      421 gctgccgtac tcaatgacat gaaatcgccg cagcgtttcc ctgaacgttg cttcatcaat
      481 gaattgctcc gcgggcgaaa ggtggcgcac gggcgattct tccgtgcgtt cggcgaacgc
      541 tttttccgcc gcttcgaatt ccttctgcac aaccgcgccg gtcgtttcga tgtcggatat
      601 ccacaccacg ttcacgttct gcccgtcgtg ccgcgccgtg aaaaaatact cgaagaacga
      661 ttcgcggctt tcggtgagca gctttccctg cacgttggga atgatggtga ggcggtcgag
      721 gttttttacc gaaagctgcg tcagcggatc gaaggtgcgg atggattcca catcatcgcc
      781 gaaaaattcg atgcggtacg gatgctcgtt cgcaaacgaa aacacatcga cgatgccgcc
      841 gcgcaccgcg tattgccccg cgtcataaac gtaatcgttg cgttcgaagc cgtattcgtt
      901 caggagatcc gtcacgaacg cgatggaaat tttttcgccg cgtttcagct gcagcgtatt
      961 tttttcgagc agctggcgcg tcacgacttt ttcagcgagc gcgccgggat aggtgacgac
     1021 agccactttt ttctcgcggc gaatacggtc gagcacttcc gcgcgcagga gcacgttcgc
     1081 gttgtcagta tcttccacct ggtacgggcg gcggtaggac gccgggaaaa agagcacggg
     1141 aaatgaagaa cgcgttcccg tttcatccat attcacgccg agaagtcctt cgagatcgtt
     1201 gaggaaataa gcagcctctt ccttatcgcc gagaatgaaa agatgggatt gcgaagaccg
     1261 ctgcaggacg gccgaggcaa aaaatgcccc tgcggaaccg gccaaacccc tgagctgaat
     1321 tcctgccgca cgcgctccgc ttatttctgt aatcacttcc ccgcaacggg gactttgctc
     1381 atacaacccg gtaatctctt caagggtcat aaggatggca aaggtaagaa gagttccatg
     1441 agcaaccgta tgtaatcgcg gatccggcag gttcgttccg cggattttta atttccaccg
     1501 taaagcttca ggaggtcatg cctttgcagc cgatttcttt tgacagaaaa acaccgcagc
     1561 aaaaaaagtt tcacacgcaa cgcgggtcac ttcgccaccg gttttctttt ttcacctgaa
     1621 accttatctt tagaattcta accaaaacca tgctcccgat gaaacgactc gccctttcct
     1681 tctgcctgat cggcttttct gcagtcacac tcttcgcaca aaccggcaaa ggctggcgcg
     1741 aagtaggcgg catgggacgc ctcgacctca attacaagaa caaggcttac aatttcgacc
     1801 ttacaccgga aatgtactgg ttcacgggtg atcatttagc gctgggaacg gatttcggcg
     1861 gaggattcgc cgccctgaag gattcttcct tttcatcgag caatgtgtat ttctatgtag
     1921 cgcccggact ccgttatttc ttcggaacgc cggataaaaa caaatggcgc ccttatcttt
     1981 tcggaaacgg cggctaccag ttcggcgcca cgcataccaa gttcaacggg acctctacca
     2041 acggaaccaa caacggtttc cgcggttacg ccggaaccgg tgtggcatgg tttttctccg
     2101 atcacgccgc attcgatatc cgcatgcgca tcgt
//
LOCUS       DASTSF010000132         3137 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1704683,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000132 DASTSF010000000
VERSION     DASTSF010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3137
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1704683"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="VFU15_03860"
     CDS             <1..198
                     /locus_tag="VFU15_03860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716937.1"
                     /translation="GSGDEKREAYSSTFGSVKFMLMQDGIRQSWGINAKRGLTEFADF
                     NFRVLKIKGSAEELPLANYPF"
     gene            203..3016
                     /locus_tag="VFU15_03865"
     CDS             203..3016
                     /locus_tag="VFU15_03865"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU4716938.1"
                     /translation="MIRSRVAFVLLCICVPLFVFGGNGDKKKKKQEEKWLEDAEYYFE
                     EGDFLRALPLYKNLSDAHPDDAYYHYQLGICYLYKDDEKDQAVVHLEKARDLEPDLPR
                     INFYLGRAYHLNYRFDDAIASFNKSMTDDNPNDKEKKEIQRYIQYCNNAKVIMQDTAD
                     VQIRNVGYPVNTDNSEYAPVISIDETTLIFTYRGVRSTGGLQDAKFRPDSTGDYYEDI
                     FVSQKVGDRWLDPDPISNNINTNHHDACVATSNDGQILFIFKSTAKDGGDLYMSTLNG
                     DVWSTPVNLGPNVNTPYWEGSCSLSSDGQLLYFASDRPGGMGGRDIYVSHRQSDGTWG
                     PAENLGPAVNTPYNDDAPFIHPDGINLFFCSEGHNSMGGYDLFYTHFKNGAWQTPVNL
                     GYPVNTPSNERYYTLTADGGTGYYSSDRRGGLGQQDIYTVTPGFQGEPPILALVVGFV
                     TVNGTPANAKIDVTDSANGNTYGNYHSNAMSGKYLIALKPGNTYKVAIEVDGFDPYFE
                     YVNVKGLDTYVQVNKDYNFVTPGDSLPRDTTQKMQPMVADSNDVLQKKIDDQLKDIRA
                     QQNDQVYEQRVYQQLLKRHGSDYDSTVNYVVDLGTYQDPKDFDSTKLTTMGPIHREVL
                     PDGMVRYSVGPFKTMLDAELYRSQLANKDSTIASNSEVVVYKSGVRQTIPSYYHTEYR
                     RKDYVPRTDTKVVMSRQGTLNTTIGSNYGYNQIVNDYGTFQADGLTYKLEISDPKDTV
                     FFAQFGKIETKTYPDGHVRYYLGPWNTLKEADDFRTNLVAKDSVAAKSLVTVFYFGQR
                     KTVPEFFASPPCNDMPVDLTWFKDKSLNDTAVYRKFLALTGNYCHDGLIYTVQIGAYR
                     HPENFKYPQLADLGPADIRNYNDSITRFTMRQFKTIREAEAFRQVVIKRGITDAWITA
                     TYKGERKTLEELIDANFYGANIQ"
BASE COUNT          802 a          798 c          841 g          696 t
ORIGIN      
        1 ggttccggcg atgaaaaaag ggaggcgtac tcttcgactt tcggatcggt gaaattcatg
       61 ttgatgcagg atggaatccg gcaaagctgg ggaattaatg caaagcgcgg gttaacggaa
      121 ttcgcggatt tcaactttcg ggtgttaaaa attaagggat cggctgaaga attgccgtta
      181 gcgaattatc cattttagcc gtatgatccg aagcagggta gcttttgtat tgctgtgtat
      241 ctgtgttccg ttatttgttt ttggaggcaa tggcgacaag aagaaaaaga agcaggagga
      301 aaaatggctc gaggacgcgg aatattattt tgaagaaggt gatttcctgc gcgcgctccc
      361 gctctacaaa aatctctccg acgctcatcc cgacgacgct tattaccatt accagctggg
      421 catctgttac ctgtacaagg atgatgaaaa agaccaggcc gtcgtgcatc ttgaaaaagc
      481 ccgcgacctg gagccagatc tcccgcggat caatttttac ctcggccgcg cctatcacct
      541 gaattaccgt tttgacgacg cgatcgccag cttcaacaaa tcgatgacgg acgacaaccc
      601 gaacgataag gagaaaaagg agatccagcg ctacatccag tattgcaaca acgcgaaagt
      661 gatcatgcag gatacggccg atgtccagat ccggaatgtc ggctacccgg tgaacacgga
      721 taattcggaa tatgcgcccg ttatttccat tgacgaaacc acgctcatct tcacgtaccg
      781 cggcgtgcgc agcacgggcg gattgcagga tgcgaaattc cgtcccgatt caaccggtga
      841 ttattacgag gatatttttg tttcgcagaa agtcggcgac cggtggctcg atcccgaccc
      901 gatcagcaac aacatcaaca cgaaccacca cgacgcctgc gtggcaacgt cgaatgacgg
      961 gcagatactt ttcattttca aaagcacggc gaaagacggc ggcgaccttt acatgagcac
     1021 actcaacggc gatgtgtggt cgacacccgt caatctcggt ccgaacgtca acacgcctta
     1081 ctgggaagga agctgctcgc tctccagcga cgggcagctg ctttatttcg ccagcgaccg
     1141 cccgggcgga atgggcggaa gggatattta cgtttctcac cggcagtcgg acggaacgtg
     1201 gggtccggcg gaaaatttgg gtccggcggt aaacactcct tataatgatg acgctccttt
     1261 tattcatccc gacggcatca atcttttttt ctgttcggaa ggccacaaca gcatgggcgg
     1321 ctacgatctt ttttacacgc atttcaagaa cggcgcatgg cagacgccgg tcaacctcgg
     1381 ttacccggtg aacacgcctt cgaatgaaag atattataca ctcacggctg acggtggcac
     1441 aggttattat tcttccgatc gccgcggcgg actcgggcag caggacattt acacggtaac
     1501 gccgggattc cagggtgagc cgcccatcct cgcgcttgtc gtcggatttg tgacggtgaa
     1561 tggaacgccg gccaatgcaa agatcgacgt gacggattcc gccaacggaa atacgtatgg
     1621 caattatcat tcgaacgcga tgtcgggaaa atatcttatt gcgctgaagc ccggcaacac
     1681 gtacaaggtc gcgatcgaag ttgacggttt cgatccgtat ttcgaatatg tcaatgtaaa
     1741 aggactcgat acgtacgtgc aggtgaataa ggattataat ttcgtcacac cgggtgattc
     1801 attgccgcgc gacacgacgc agaagatgca gcccatggtg gcggattcga atgatgtgct
     1861 gcaaaagaag atcgacgacc agctgaaaga tattcgcgcg cagcagaacg accaggtgta
     1921 tgagcagcgc gtttaccagc agctgctgaa aaggcacggc tccgattacg attcgacggt
     1981 gaactacgtc gtcgatctcg gtacctacca ggacccgaag gatttcgatt cgacgaagct
     2041 gacgacgatg ggaccgatcc accgcgaagt tttacccgac ggcatggtca ggtattcggt
     2101 ggggcctttc aagacaatgc tggatgcgga actgtatcgc agccagctgg cgaataaaga
     2161 ttccaccatt gcgtccaatt ccgaagtggt ggtctacaag agcggcgtcc gccagacgat
     2221 cccctcgtat tatcacaccg aatacaggcg gaaggattat gttccgcgta cggatacgaa
     2281 ggtggtgatg agccgccagg gaacgctcaa cacgacgatc ggatcgaatt acgggtacaa
     2341 ccagatcgtg aatgattacg gaactttcca ggcggacggg cttacctaca agctcgaaat
     2401 ttccgatccg aaagacacgg ttttctttgc acagttcggg aagatcgaaa cgaaaaccta
     2461 tcccgacggt cacgttcgtt attacctcgg accgtggaat acgctgaagg aagcggatga
     2521 tttcaggaca aacctggtcg cgaaagattc ggttgccgcg aaatcgctgg tgacggtgtt
     2581 ttatttcggg cagcgtaaaa cggttcccga attctttgcc agcccgccgt gcaacgacat
     2641 gccggtcgat cttacgtggt tcaaggataa atcacttaac gacacggccg tttaccggaa
     2701 attcctcgcg cttacgggga actattgcca cgacgggctc atttacaccg tgcagatcgg
     2761 cgcttaccgc catcctgaaa atttcaaata cccgcagctg gcagacctgg gtccggccga
     2821 catccggaat tacaacgaca gcatcacgcg tttcacgatg cgccagttca aaacgatccg
     2881 cgaagcggaa gcattccgcc aggtggtcat caaacgtggc attaccgatg cgtggatcac
     2941 cgcaacgtac aagggagaac ggaaaacact cgaagaactg atcgacgcga atttctacgg
     3001 ggcgaatatc cagtaggacg gttgccggtt gccagtttcg attccgccgt ttgttgtttc
     3061 cctcgcgttc tccatgaaaa accttcgtgc gctttgtgaa aaaacttctt gtgctttgtg
     3121 aaaaaacttc ttgtgct
//
LOCUS       DASTSF010000133        11410 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_810406,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000133 DASTSF010000000
VERSION     DASTSF010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 11410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11410
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_810406"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1162)
                     /locus_tag="VFU15_03870"
     CDS             complement(<1..1162)
                     /locus_tag="VFU15_03870"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HEU4716939.1"
                     /translation="MSQVELIIPRLGESVNEATIIKWLKNEGDKVSADEPVLEIATDK
                     VDTDVPSPYSGILAKQLFKEGDVVAVGKAVAMISTEAGAAVTQAPVNTPPAEEKKAEI
                     VSAEVKAQVAVSAEQSVKSSGTSRFYSPLVKNIAKKEGISVQELEAIEGTGMHGRVTK
                     NDILNYIPNKGQLHTQVQQQVQEASVISNGNGSAGKKETVQPVQQMQKPNVTMMNGDE
                     IVEMDRMRKLIADHMIMSKQTSAHVTSYVEADVTNLVLWRNRVKNAFEKRENEKLTFT
                     PMFVEAVVKAIKDFPMINVSVDGTKIIVRKNINIGMAAALPSGNLIVPVIRNADRMNL
                     FGLAATVNDLAGRARAGKLLPDEIQGGTYTISNVGTFGNVMGTPIINQPQVAI"
     gene            1340..2119
                     /locus_tag="VFU15_03875"
     CDS             1340..2119
                     /locus_tag="VFU15_03875"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HEU4716940.1"
                     /translation="MKGKLGRKFQEEIFSERCQNIVRLLRSYRKKHSQETIHRLRVEL
                     KRVNAFFILFDPQRKGRPGKTFASFRRVFRKAGKIRSAYIHLQVLHDLKTPSGPFIEN
                     LRTSMLKDARRFAANAAKRAERIGKYPRLFAHAADGVKTEKIVRFFEKEMAAIGRKLK
                     KDTGEESLHDLRARMKNLLYLHSFLPGRLVEKIRMDTSYLHSLQDEIGKWHDVLDTIK
                     VVRRAGGKSKSLENRLSSEADARLKDILLHAADFHPKITGR"
     gene            complement(2239..2946)
                     /locus_tag="VFU15_03880"
     CDS             complement(2239..2946)
                     /locus_tag="VFU15_03880"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018710404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEU4716941.1"
                     /translation="MHLFYVPDLSGDSVYLSEEESKHAVRVLRLKNSDIVELVDGKGT
                     RAYGRIANDHPKRCGISITEKKTELHSRNYSIHLAIAPTKNAERIEWMLEKCTETGLD
                     EITFLDCENSERSRINMERMEKVAVSAMKQSRQSRLPLIHPLTDFEKFISQDFHGQKF
                     IAHCSGNERKNFAAAVGKGKNVLVLIGPEGDFSPEEIRLADSNGFGDVSFGDNRLRTE
                     TAGLFAVAGIHIVNAAP"
     gene            complement(2961..3812)
                     /locus_tag="VFU15_03885"
     CDS             complement(2961..3812)
                     /locus_tag="VFU15_03885"
                     /inference="COORDINATES: protein motif:HMM:NF012951.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HEU4716942.1"
                     /translation="MPFLKSRIAPTPSGFLHAGNAFSFLLADTLTHDHGGKLLLRIDD
                     LDTERKRDEFVEDIFESLRWLGITWDEGPADAADFEKNWSQKTRMTDYHALLGKLSGT
                     GRVFACSCSRKQLALPSFRCECHLKNIPLQTENVSWRIHVPANCVVEFDDIYLGKCSV
                     DLAEVMGPFVIRRRDGIPAYQVASLADDLHSGINFIVRGNDLLHSTAAQVYLASLAGE
                     NDFGKNLFLHHPLVEDDSGSKLSKSAGASSLRAMRMNGVPAEMVKTAFRKWRNGIQIN
                     LRKFEDR"
     gene            complement(3840..5054)
                     /locus_tag="VFU15_03890"
     CDS             complement(3840..5054)
                     /locus_tag="VFU15_03890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716943.1"
                     /translation="MPKLFQILLLAFFLVPSRIFAIDGKDSVNSSPAPSRTRAREVRN
                     YIRPCIYFNYYGTKPRAVKGTNHTLNSRLGDYSYAQSNLGCYVPLYTHTAFGKRDTTD
                     VNTFHLLMTFNALTDMPKFSGLDEQHKIYKGGLGLRAIYGFGSQYVLFADISPYVVED
                     RFDKQQTQHVRLASSFVFNWMITPAFSMRFGGTRTFLLGNRFYLPMIGFRVGRLDSKF
                     YFSFQFPRYTSFNFQPSPKFSFSFYSKSMGGLYNFSNGDSIYNGRDSVIQFGQFGVAH
                     GVRFDFRTGPDFSCFVSAGFESSNHIWFYSYSFNRDNHLGPLHHFYDGRPAPVGFLNF
                     GVTWRFGQAKKSTGNYLMYDIFDLNNTMDPGDNNSGPGNGDVTPQYDKKKEMRKVQYN
                     DVVDLLDDSDLN"
     gene            complement(5060..5569)
                     /locus_tag="VFU15_03895"
     CDS             complement(5060..5569)
                     /locus_tag="VFU15_03895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716944.1"
                     /translation="MKRIFYLIVIFLALAAGDAVAQTGGGSSKTDTTKSTASGAERVA
                     ALKVAFITKQLNLTPEEAEKFWPIYNEYQAKRDAIHKQEQENRDKIRTQADQLSPDEL
                     QKCADLEISLRQQDMALQVEMHEKLKAVLPPKKLAMLYVAEEDFKKELLRILTTDTAT
                     GSKGDKGQK"
     gene            complement(5574..6668)
                     /locus_tag="VFU15_03900"
     CDS             complement(5574..6668)
                     /locus_tag="VFU15_03900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716945.1"
                     /translation="MNEWNDNLPADFEDLSKHAPLLDKLRAKGDGFAVPENYFSEALS
                     VIESLVRLPGKNGMTVPENYFTDLAQHIISSAELAKLKDENPLSVPENYFEALADDIT
                     SHAALKSISENEFVIPENYFTGFDDTLNTKIALDNIRQDDGFSTPEGYFEKLSSSVMA
                     RVAMDDLSKGSDADVPEGYFDTLAGRIAEKIAAEENGPEKNRRRGRVIVFAEVLRRYA
                     KPAAIAASAALLILAGTWFVNHSAGGELKNNSVASHTHAPVSPLPAPPSPVIQPGPAQ
                     QDVAVNKPRKKKNHSRVNHEQGQVVKVEKQDVMQSLDQLDENIVADFVADQKPDVNKP
                     ADPPAMNDAMYNYLLNNNADLGDLIKDSNK"
     gene            complement(6672..7229)
                     /locus_tag="VFU15_03905"
     CDS             complement(6672..7229)
                     /locus_tag="VFU15_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012787861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU4716946.1"
                     /translation="MSERSDKELIAQFRDEKTRQGAFRLIVERYQKRLYWHIRRMLVD
                     HDDTDDVLQNVFIKVWKGLDGFKEDAQLYTWLYRIATNETLSFIRQRKADLFVRYGDV
                     EYNIESRLEDDNYFKGDEIQKKLHTAIQQLPEKQRLVFTMKYFEDIRYEDMSDILETS
                     VGALKASYHHAVKKIEAYIRTTAEQ"
     gene            complement(7284..8249)
                     /locus_tag="VFU15_03910"
     CDS             complement(7284..8249)
                     /locus_tag="VFU15_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013631265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEU4716947.1"
                     /translation="MKEYTYTEKKDTRSGFGAGLLELGRKNSNVVALCADLTGSLKMD
                     AFEKEFPTRFFQVGIAEANMIGIAAGLTVGGKIPFTGTFANFSTGRVYDQVRQSVAYS
                     GKNVKICASHAGLTLGEDGATHQILEDIGLMRMLPGMTVINTCDFNQTKAATLAIAEL
                     EGPVYLRFGRPSVPNFTPADQSFRIGKAVHLQEGTDVSIFATGHLVWKALEACKILAG
                     KGISAEIINIHTIKPLDEEAVLQSVRKTRCAVSAEEHNKFGGLGECIANVLSKHTPAP
                     LEMVAVNDSFGESGTPDELMKKYGLDTPDIVKAAEKAVSRKNSRS"
     gene            complement(8320..9723)
                     /locus_tag="VFU15_03915"
     CDS             complement(8320..9723)
                     /locus_tag="VFU15_03915"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEU4716948.1"
                     /translation="MKKLSPGFLKIFLLAAAVFFSTSFPAQTAPTGGQQLTRILFVFD
                     ASFSMFGQWQSGSKMEIAKRLLSNFLDSLKNTKNLEMGLRCYGHQHNLTPTRDCQDTK
                     LEVPIAKPSVSIPAMQNKIAQLVPRGTTPIAYTLEQCGGDFPKDGKNARNIVILITDG
                     IEECDGDPCAVSLSLQQQGIILKPFVIGVGLSGDYSTLGCIGHFYDVSQENTFTNVLN
                     IVVSEALNNTTCQVNLLDLAGKPTETDVAMTFYDQQTGVVRYNYMHTMNNRGVPDTIT
                     LDPLSTYKLVVHTIPEVVKENITVTPGKHNIIAVDAPQGYLNLQVAGINNYSRLQCII
                     RKAGTMETVNVQDFGRIEKYIVGKYDLEILSLPRIYMYNVDVGQSKTTLVQIPQAGVV
                     NIAKPSEGTGQILLDDKNKLTFVCNLNDQMLNESVVLQPGNYRVIYRSKSAHETVYTV
                     EKKFKIDPGGQAAVKLY"
     gene            complement(9730..10578)
                     /locus_tag="VFU15_03920"
     CDS             complement(9730..10578)
                     /locus_tag="VFU15_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014033561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEU4716949.1"
                     /translation="MPSTQELERLCSQVRRDILRMVHAVNSGHPGGSLGCTEYFVALY
                     NEIMKHDPSRFTMDGIGDDVFFLSNGHISPVFYSVLARAGYFPVEELKTFRRLNSRLQ
                     GHPTTHEHLPGVRIASGSLGQGMSVAIGAALAKKLNNDDRIVYSLHGDGEMDEGQVWE
                     AAMYAGSRKVDNLICTVDVNGQQIDGPTEKVMPLGDLGAKFLAFGWDVMKIADGNNIS
                     EVISALKSAREHTGKGKPVVILMKTEMGCGVDFMAGTHKWHGVAPNDEQLAQALKQLP
                     ETIGDY"
     gene            10786..>11410
                     /gene="ychF"
                     /locus_tag="VFU15_03925"
     CDS             10786..>11410
                     /gene="ychF"
                     /locus_tag="VFU15_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007661265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEU4716950.1"
                     /translation="MSLKCGIVGLPNVGKSTLFNCLSNAKAQAANFPFCTIEPNIGVI
                     TVPDERLNKLAELVKPERIVPTTVEIVDIAGLVKGASKGEGLGNKFLANIRETNAILH
                     VLRCFDDPNVVHVDGSVNPVRDKEVIDLELQLKDLESVEQRMKRIEKQAKTGDKDSKK
                     TYDILVQMNDALLKGKSARTVNISPEDAKLIADLHLLTAKPVLYVCNV"
BASE COUNT         2397 a         2999 c         3161 g         2853 t
ORIGIN      
        1 ggatcgccac ctgcggctgg ttgatgatcg gcgtgcccat gacgttgccg aatgtgccga
       61 cgttggagat cgtgtaggtt ccgccctgga tttcgtcggg aaggagcttc ccggcgcgtg
      121 cgcggccggc gaggtcgttg accgtggcgg caagaccgaa aaggttcatg cggtctgcgt
      181 tgcggatgac aggaacgatg aggttcccgc tgggcagcgc ggccgccatc ccgatattga
      241 tgttcttccg cacgatgatc ttcgttccat cgacggaaac gttgatcatg ggaaaatcct
      301 tgatggcttt cacgacggct tccacgaaca tcggtgtgaa cgtgagcttt tcattctcgc
      361 gtttctcgaa tgcgttcttc accctgttcc gccagagcac gaggttcgtc acgtcggctt
      421 ccacgtagga agtgacatgc gcggatgtct gcttgctcat gatcatgtga tcggcaatga
      481 gcttgcgcat gcggtccatc tccacgatct cgtcgccgtt catcatcgtc acgttcggct
      541 tctgcatttg ctgtaccggc tgaacggttt cctttttacc ggcggaaccg ttcccgttgg
      601 aaataaccga agcttcctgc acctgctgct gcacctgtgt gtgcagctgt cctttgttcg
      661 ggatataatt gaggatatcg tttttcgtta cacgcccgtg cattcctgtt ccttcgatgg
      721 cttccagctc ctgcacggaa atgccttctt tcttcgcgat gttcttcacc agcggtgaat
      781 agaaacgcga agtgccggag gattttacgg attgttcggc ggaaacggcg acctgcgctt
      841 tcacttcagc agaaacaatt tcagcttttt tctcttctgc aggaggcgtg ttcacaggcg
      901 cctgtgtaac cgccgcaccg gcttcggtcg agatcatggc caccgctttg cctacggcga
      961 caacgtcacc ttctttgaaa agctgtttcg ccaggatgcc gctgtatggc gagggaacgt
     1021 ccgtatccac cttatccgtc gcgatctcga gcaccggctc gtcggctgac actttatcgc
     1081 cttcgttctt cagccatttg atgatcgtgg cctcgttcac gctttctccc agacggggaa
     1141 tgatcagttc tacctgtgac ataattgttc tgctgaaatg ttgggaactt tacggcctct
     1201 gcagccggct gcaaaaatac ggttttcccc tgacagattt ccggcacgtc gtggcgttgt
     1261 gcgcgttttt tccgggcggc agagccagaa ttccggcagg cgtaacatga cgcagcgggg
     1321 gaatagttac tttcagaaaa tgaaagggaa gctcggcaga aaattccagg aggagatttt
     1381 ttctgaacga tgtcagaata tcgtccggct gctcaggtcg tacaggaaga agcattccca
     1441 ggaaaccatt caccgcttgc gcgtggagct gaaaagggtc aacgcttttt tcatcctgtt
     1501 cgatccgcag cgtaaaggcc ggcccggaaa aacgttcgca tcgttccgcc gggtttttcg
     1561 caaggcagga aagatccgct cagcctatat tcatctccag gtgctgcacg atctgaaaac
     1621 gccttccggt ccgttcatcg agaaccttcg cacttccatg ctgaaggacg ccaggcgttt
     1681 tgcggcgaat gcagcgaagc gcgcggaacg gatcgggaaa tatccgcgcc tttttgcgca
     1741 tgcagcagat ggcgtaaaaa cggagaagat cgtgcggttt ttcgaaaaag agatggcggc
     1801 gatcgggcgg aagctgaaga aagataccgg cgaagaatcg cttcacgatc tccgtgcgcg
     1861 catgaagaac ctgttgtatc tgcattcgtt tcttccgggg aggctggtgg aaaaaatccg
     1921 catggatact tcttatctcc attcgctgca ggacgagatc gggaagtggc acgatgtgct
     1981 cgacacgatc aaggttgtgc ggcgtgcggg agggaaaagc aaatcgctgg aaaacaggct
     2041 ttcgtcagaa gcggacgcac ggctgaaaga tattctcctt catgcggcgg attttcatcc
     2101 gaaaatcacc gggcggtaaa gcttacaggc aattccggaa aatttttacc ctgttttttt
     2161 gcgaagcgaa tataattgcg ggcacccgtg gcagctcttc tgtttcaatc ctgccgctgt
     2221 acgcagttgt tttttcattc acggcgctgc gtttacgata tgaattcctg caacggcgaa
     2281 taatcccgcg gtttccgtcc gcagccggtt atcgccaaag gaaacatccc cgaagccgtt
     2341 cgagtcggcg agacgtatct cttccggtga gaaatcgcct tcgggaccaa taagcaccag
     2401 cacgtttttc cctttcccga ccgcggcggc gaaatttttg cgttcgttcc cggaacaatg
     2461 cgcgatgaat ttctgcccgt gaaaatcctg cgaaataaat ttttcaaaat cagtcagcgg
     2521 gtgaatgagc ggaagacgtg attgcctcga ctgcttcatc gcggagacgg caactttctc
     2581 catccgttcc atgttgatgc gtgaacgttc tgaattttcg cagtcgagaa acgtgatctc
     2641 atcgagcccg gtttccgtgc atttttccag catccattct attcgttccg cgttcttcgt
     2701 cggtgctatc gcaaggtgga tggaataatt acgcgaatgc agctccgttt ttttctccgt
     2761 gatggaaatg ccgcagcgtt tcgggtgatc gttcgcgatg cgtccgtaag cccgcgtgcc
     2821 tttcccgtcg acgagttcga cgatgtcgct gttcttcagg cggagcacac gtacggcgtg
     2881 cttcgattcc tcttcgctga ggtaaacgga atcgccggaa aggtcaggga cgtaaaagag
     2941 gtgcataacg gaagcgggag ctatcggtct tcaaatttcc gcaaattaat ctgaatcccg
     3001 tttcgccatt tgcggaaagc ggttttcacc atttccgccg gaacgccgtt catgcgcatt
     3061 gcccgcagcg atgacgcgcc cgccgatttg gaaagcttgc tcccggaatc gtcttcgacc
     3121 agcgggtgat gcaggaaaag attctttccg aaatcatttt ccccggccag tgaggcaaga
     3181 taaacctgtg ctgccgtgga atgcaggaga tcatttccgc ggacgatgaa attgatgccg
     3241 gaatgaagat cgtcggccag cgaagcaacc tggtacgcgg gaatcccgtc gcggcggcgg
     3301 atcacaaacg ggcccatgac ctctgcaaga tccacgctgc atttcccgag ataaatgtcg
     3361 tcgaattcca cgacgcagtt cgccggaacg tgaatgcgcc aggaaacatt ctccgtttgg
     3421 agcgggatgt ttttcaaatg acattcgcag cgaaaagagg gaagcgcaag ctgcttacgg
     3481 gaacagctgc acgcaaacac gcggccggtg ccggaaagtt ttccgagcaa cgcgtggtaa
     3541 tctgtcatgc gcgttttctg cgaccagttc ttttcaaaat ccgctgcatc cgccggacct
     3601 tcgtcccacg ttattcccag ccagcgcagg ctttcgaaaa tatcttccac gaattcatcc
     3661 cgtttccgtt ctgtgtcgag atcatcgatg cgcaggagca gtttcccgcc gtgatcatgt
     3721 gtcagcgtat ctgccagcag gaaggaaaac gcattgcccg catgaagaaa tccgctcgga
     3781 gtgggagcga tacggctttt gaggaacggc atgggcaaat gtaccgtatc gggaagaaat
     3841 cagttcaggt ccgaatcatc cagcaggtcg accacatcat tgtactgcac cttccgcatt
     3901 tcctttttct tatcatactg cggcgtaacg tcaccattgc cgggaccgct gttgttgtcg
     3961 cccggatcca tcgtgttgtt gaggtcgaaa atgtcgtaca tgaggtaatt gccggttgac
     4021 tttttcgcct ggccgaaacg ccacgtcacg ccgaaattga ggaagccgac cggggccggt
     4081 ctgccgtcgt aaaaatgatg cagcgggccg aggtgattgt cccggttgaa cgaatacgaa
     4141 tagaaccaga tgtgattgga tgattcgaat ccggcagata cgaaacagga aaaatcaggt
     4201 cctgtgcgga agtcgaaccg aacgccgtgc gccacgccga actgtccgaa ctgtatgacc
     4261 gaatcgcgcc cgttgtagat ggaatcgccg ttgctgaaat tgtacaatcc gcccatcgat
     4321 ttgctgtaaa agctgaatga gaatttcggc gagggctgga agttgaacga cgtatagcgc
     4381 gggaactgga aagagaaata gaatttgctg tcgaggcggc cgacgcggaa cccgatcatc
     4441 ggaaggtaaa aacggttgcc cagcaggaac gtccgtgttc cgccgaagcg catgctgaat
     4501 gcgggcgtga tcatccagtt gaaaacgaac gatgaggcaa gacgaacgtg ctgcgtctgc
     4561 tgcttgtcga acctgtcttc gaccacgtac ggcgaaatgt ctgcgaacaa aacatactgc
     4621 gaaccgaagc cgtagatcgc gcgaagcccg agtccgcctt tgtaaatttt atgctgttca
     4681 tcgagacccg aaaatttcgg catgtcggtg agcgcgttga acgtcatcag gaggtgaaag
     4741 gtattgacat ccgtcgtgtc cctttttccg aaagccgtat gcgtgtagag cggaacatag
     4801 cagccgaggt tcgactgtgc gtagctgtaa tcgccgagcc ggctgttcag cgtgtggttc
     4861 gttcctttca ccgcccgcgg cttggtcccg tagtaattga aataaatgca ggggcggatg
     4921 taatttctca cctcgcgtgc gcgcgtgcgc gaaggcgccg gcgagctgtt cacgctgtct
     4981 tttccgtcaa tggcgaaaat cctggacggg accaggaaaa aggcaagcag gagtatctgg
     5041 aaaagtttcg gcatacgatc tatttctggc ctttatcgcc tttgctgccg gtggcggtat
     5101 ccgtcgtgag aatgcgcagc agctcttttt tgaaatcttc ttcggccaca tacagcatcg
     5161 ccagtttttt cgggggaaga acggctttca gcttttcgtg catttccacc tgtaaggcca
     5221 tgtcctgctg ccgcaatgaa atttcgaggt cggcgcattt ctgcagctca tccggggaaa
     5281 gctggtcggc ctgtgtgcgg atcttgtcgc ggttttcctg ttcctgcttg tgaatggcat
     5341 cgcgtttcgc ctggtactcg ttgtagatgg gccagaattt ttcggcttct tcgggcgtaa
     5401 ggttgagctg cttggtgatg aaagcgactt tcagggcggc cacgcgttct gcgccggagg
     5461 cggtgctttt cgtcgtatcg gttttcgacg atccgccccc cgtctgcgca actgcatcgc
     5521 ccgctgcaag cgccaggaaa atcacgatca ggtaaaaaat ccgcttcatc aggttacttg
     5581 tttgaatctt tgatcaaatc tccgaggtct gcgttgttgt tcagcagata attgtacatc
     5641 gcgtcgttca ttgcgggcgg atcagcgggc ttgttcacgt ccggtttttg gtccgccaca
     5701 aaatccgcta ctatgttttc atccagctgg tccagcgatt gcatcacatc ctgcttttca
     5761 actttgacga cctgtccctg ctcatggttt acgcgggaat gatttttctt tttgcgcggc
     5821 ttgttcacag ccacatcctg ctgggcaggt ccgggctgaa taaccggcga aggcggtgca
     5881 ggcaggggag aaacaggtgc atgcgtgtgt gaagcgacgc tgttgttttt caattccccg
     5941 ccggcggaat gattgacgaa ccatgtcccg gcgagaatga gcagtgcggc ggacgcggcg
     6001 atggcggccg gtttcgcgta acgcctcagc acttcggcga aaacgatcac gcggcctctg
     6061 cgcctgtttt tttccgggcc gttttcttcc gcagctattt tttctgcaat gcggccggcg
     6121 agcgtatcga aataaccttc cggcacatcg gcgtctgaac ctttggaaag atcgtccatc
     6181 gcgacacgcg ccatgacgga agaggaaagc ttttcaaaat atccttcggg tgtgctgaaa
     6241 ccgtcgtcct ggcgaatgtt gtccagcgcg atcttcgtgt tcagcgtgtc gtcgaaaccg
     6301 gtgaaataat tttcgggaat gacaaattcg ttctccgaaa tgcttttcag cgcggcgtgc
     6361 gacgtgatgt cgtccgcaag agcttcaaaa taattttccg ggacggagag cgggttttcg
     6421 tccttcagct tcgccagctc tgcggatgaa atgatgtgct gcgccaggtc cgtgaaatag
     6481 ttttccggaa cggtcatccc gtttttcccg ggcagacgta caaggctttc gatgacggaa
     6541 agagcttcgc tgaaataatt ttccggaacg gcaaacccgt ctcccttcgc gcggagcttg
     6601 tccagcaaag gagcgtgctt cgaaaggtct tcgaaatccg ccggcaggtt atcgttccat
     6661 tcattcatcg tttactgttc agctgttgtg cgtatgtagg cttctatttt ttttaccgca
     6721 tgatggtagg acgctttcag tgcgcccacc gaagtttcga gaatgtccga catgtcttcg
     6781 taacgtatgt cctcgaaata tttcatcgtg aaaacaagac gctgcttttc gggaagctgc
     6841 tgtatcgcgg tgtgcagctt tttctggatc tcgtcgcctt tgaaatagtt gtcgtcttcc
     6901 agcctgcttt cgatgttgta ttccacatcg ccgtagcgca cgaaaagatc ggctttgcgc
     6961 tggcggatga agctgagtgt ttcattggtg gcgatgcggt aaagccaggt gtacagctgc
     7021 gcatcttcct tgaacccgtc gagacctttc cacactttga taaaaacatt ctggagaacg
     7081 tcgtccgtgt cgtcatgatc caccagcatg cggcggatgt gccagtacag ccgtttctgg
     7141 taacgctcca cgataaggcg aaacgcaccc tgccgtgttt tttcgtcacg gaattgtgca
     7201 atcagttctt tatcgctgcg ttcgctcata aaaaaagctg cgtggtgaaa ttcccgcgca
     7261 gcctgaaatt agtgaatcac tttttagctc cggctgtttt tcctgcttac cgctttttca
     7321 gccgccttca cgatatccgg cgtatcgagc ccgtattttt tcatcagttc gtcgggtgtt
     7381 ccgctttctc cgaatgaatc atttacggca accatttcga gcggggcggg agtgtgcttt
     7441 gaaagaacgt tggcgatgca ttccccgagt ccgccgaatt tgttgtgctc ttctgctgaa
     7501 actgcacagc gggtctttcg taccgattgt aagacggcct cttcatccaa aggtttaatg
     7561 gtatggatgt ttattatttc tgcagaaatg cctttccccg caagaatttt acaggcttcc
     7621 agcgctttcc acacaaggtg gcccgtggcg aagatcgaaa cgtccgtacc ttcttgtaaa
     7681 tgaactgctt tcccgatcct gaacgactgg tcagccggtg tgaaattagg cacggaaggc
     7741 cggccgaagc ggaggtaaac gggcccttcg agctctgcaa tggcgagggt ggccgctttg
     7801 gtctggttga aatcgcacgt attgatgacc gtcatgccgg gcagcatgcg catcaggccg
     7861 atgtcttcga ggatctggtg cgtggcgccg tcttccccga gcgtaagtcc cgcatgcgat
     7921 gcgcagatct tgacgttctt gccggaataa gcgaccgact ggcggacctg gtcatacaca
     7981 cgccccgtgg aaaaattggc gaaggtgccg gtgaacggga tttttccgcc taccgtgagt
     8041 ccggcggcga taccgatcat gttcgcttca gcaattccta cctggaaaaa acgggtggga
     8101 aattcttttt cgaaagcgtc cattttcagc gaaccggtga gatcggcgca cagcgccacg
     8161 acgttgctgt tcttccttcc gagctcgaga agcccggcgc cgaagcccga acgcgtgtct
     8221 tttttttccg tgtaggtgta ttctttcatg gatgtgtgat tctctgtttt cagtgcccgt
     8281 tttttctctt tttttcgtcc ctcatctctg gtccggcgtc taatacaact tcactgccgc
     8341 ctgtccgccc ggatcgatct tgaatttttt ctcgaccgtg tatacggttt catgcgccga
     8401 ttttgaacgg tagatcacgc ggtaattgcc gggctgcagc acaacgcttt cattgagcat
     8461 ctggtcgttc aggttgcaga cgaacgtgag cttgttcttg tcgtcgagca atatctgccc
     8521 cgttccttcc gaaggtttgg cgatgttcac cacgccggcc tgtggtatct gcacgagcgt
     8581 cgttttgctc tggccgacat cgacgttgta catgtagatg cggggcagcg aaaggatctc
     8641 gaggtcgtat ttgccgacaa tgtatttctc gatgcgaccg aagtcctgca cgttcacggt
     8701 ttccatcgtt cctgccttgc gtatgatgca ttgcaggcgg gagtaattat tgattcccgc
     8761 cacctgcaga ttgagatagc cctgcggcgc atccaccgcg atgatgttgt gcttcccggg
     8821 cgtaaccgtg atgttttcct tcaccacttc gggtatggtg tgcacgacga gcttgtatgt
     8881 gctcagcgga tcgagcgtga tggtgtccgg aacgccccgg ttgttcatcg tgtgcatgta
     8941 gttgtagcgc actacgcccg tctgctggtc gtaaaaggtc atggcaacat ccgtttccgt
     9001 cggcttcccg gcaaggtcga gcaggttgac ctggcacgtg gtgttgttca gcgcttcgga
     9061 gacgacgatg ttgagaacgt tggtgaatgt attttcctgc gaaacgtcgt aaaaatggcc
     9121 gatgcagccg agcgtggaat aatccccgct gagtccgacg ccgatcacga acggcttgag
     9181 gatgatgccc tgctgctgca gcgaaagcga aacggcacag ggatccccgt cgcattcttc
     9241 gatgccgtcg gtgatgagga tgacgatgtt gcgtgcgttc ttcccgtcct tgggaaaatc
     9301 gccgccgcat tgttcgagcg tgtacgcgat cggggttgtt cctctcggca caagctgcgc
     9361 gatcttgttc tgcatggccg gaatggaaac ggatggtttc gcgatcggaa cttccagctt
     9421 cgtatcctgg cagtcgcgcg tgggcgtgag gttgtgctgg tgcccgtagc agcgaagccc
     9481 catctcgagg tttttcgtgt tcttcagcga atcgagaaaa ttgctgagca gccgcttggc
     9541 aatttccatc ttgcttccgg attgccattg cccgaacatg ctgaacgaag cgtcgaagac
     9601 gaacaggatg cgcgtcagct gctgcccgcc cgtgggagcg gtctgcgcag gaaatgacgt
     9661 gctgaaaaaa acagcagccg caagcaggaa gattttcagg aacccggggg acagtttttt
     9721 catcatagct cagtaatcac cgatcgtttc aggcaattgt ttcaaagcct gtgccaactg
     9781 ctcgtcgttc ggggcgacgc catgccactt gtgtgttccg gccatgaaat cgacgccgca
     9841 gcccatttcc gtcttcatga gtataacgac cggcttgcct ttgccagtat gttctctggc
     9901 tgatttcagc gcggaaataa cttctgaaat gttgttcccg tccgctattt tcatcacgtc
     9961 ccatccgaag gcgaggaatt tcgcgccaag gtcgccgagc ggcatcactt tctccgtggg
    10021 cccgtcgatc tgctgaccgt tcacgtccac cgtgcagatc agattatcca ctttgcgcga
    10081 gccggcgtac attgccgctt cccacacctg gccttcgtcc atttccccgt cgccgtgcag
    10141 cgaatacacg atgcggtcat cgttgttgag ttttttggcc agcgccgcgc cgatggcaac
    10201 ggacattccc tgcccgagcg agcccgatgc gatacggacg ccgggaagat gctcgtgcgt
    10261 cgtcgggtgt ccctgcagcc gggagttgag cctgcggaag gttttcagct cttcgacggg
    10321 aaaatatcct gcgcgggcaa gcacgctgta aaacacgggc gagatatggc cgttcgaaag
    10381 aaaaaaaaca tcgtcaccga taccgtccat cgtgaagcga gaaggatcgt gcttcatgat
    10441 ctcgttgtac agcgcgacaa aatattctgt gcagccgagc gaaccgccgg ggtggccgga
    10501 gttgacggca tgaaccattc gtagaatgtc gcggcgaacc tgcgaacaga ggcgttcaag
    10561 ttcctgggtg gaaggcatag aaatctggat ggacgaaaaa ctgtggccaa aagtacacct
    10621 tgtcgttagg gcataattcc tatgttaata acattcgggt gttgttgaaa actacgaggt
    10681 gtaattgact ttccctgctg aaatccgaaa ttccgaaatg gtatttttgc ccccctgaga
    10741 gggagcatgt aaagtacatt tcccccaaac acccgaaaaa attttatgtc cctgaagtgc
    10801 ggcatcgtgg gtcttcctaa cgtgggtaaa tcgacccttt tcaattgcct gtcaaacgca
    10861 aaggcgcagg cagcgaattt tccgttctgt acgatcgaac cgaatatcgg cgtcatcacc
    10921 gttcccgacg agcggctgaa caagctggcg gagctggtca agcccgaacg catcgttccc
    10981 accacggtgg agatcgtcga cattgccggg ctggtgaaag gcgcgagcaa aggcgaagga
    11041 ctcgggaata agtttctcgc caacatccgt gagacgaacg ccatcctgca cgtactccgc
    11101 tgcttcgatg atccgaacgt ggtgcatgtg gatggttcgg tgaatcctgt ccgcgacaag
    11161 gaagtgatcg atctcgagct gcagctcaaa gacctggaat ccgtcgagca gcgcatgaag
    11221 cgcattgaaa agcaggcgaa gaccggcgac aaggattcga aaaaaacata cgacatcctc
    11281 gtgcagatga acgacgcgct gctgaaagga aaatcggcgc gcaccgtgaa catttcgccc
    11341 gaggacgcga agcttattgc tgatcttcac ctgctcacgg ccaagcccgt gctctacgtg
    11401 tgcaacgtgg
//
LOCUS       DASTSF010000134         4885 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_587493,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000134 DASTSF010000000
VERSION     DASTSF010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4885
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_587493"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..720
                     /locus_tag="VFU15_03930"
     CDS             <1..720
                     /locus_tag="VFU15_03930"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU4716951.1"
                     /translation="TVRCRLVSLQDKEAVLEFSVQDTGIGIAPDKIEKVFESFTQASS
                     DTNRKFGGTGLGLTISRQLVELMGGKLTARSTPGEGTTFTFTVSLPVGEGGTRQATEE
                     VLQLDMIRRAKLLLVEDNEFNRIVAVDTLNEFLGEVQVDVARDGNEALGKIAQNSYDL
                     VLMDIQMPGMDGYDATRHIRKMASPLNKIPILAMTANATPEEIAKCSECGMDGHVAKP
                     FVPEALFRKMCELLNARDANA"
     gene            742..1101
                     /locus_tag="VFU15_03935"
     CDS             742..1101
                     /locus_tag="VFU15_03935"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HEU4716952.1"
                     /translation="MSDENKTQTDLGFLGTFTGNDTARMRKYITLFLGAAPAEAEAIR
                     SALTTADHEKVRASAHSLRPQMTYMGAKAGEELLKRMEEDVKAGKYDELQVTGAKFEA
                     VFSKVCEELQAYLKQIS"
     gene            1128..1646
                     /locus_tag="VFU15_03940"
     CDS             1128..1646
                     /locus_tag="VFU15_03940"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU4716953.1"
                     /translation="MKEVNTIFIVDDDATQLTMVADHLSKFKNITIKTFSSGEECIKN
                     LSLKPQVIFLDYNFDKAGDKAKNGIDVLKSIKDADPSIEVVMLSGQDRIEVAVNTMKY
                     GAFDYIVKNESSFHRAENAVKNIIRKIRLEGNVKIYKRLFITFAICFTLMIAIIIYLW
                     ANGYIAKNPGWF"
     gene            2074..2787
                     /locus_tag="VFU15_03945"
     CDS             2074..2787
                     /locus_tag="VFU15_03945"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEU4716954.1"
                     /translation="MSTKSASLNCLVVDDDDMVRIDIGQKISDTPFLHLAGSCASALE
                     AAAVMQKEKIDLVFLDILMPEMTGLQFMESMGEKSIQVIFITSEKDYAAQAFDLDVTD
                     FLVKPVSMERFLKAVMRASSNVKTTGGDAVRNNHLFVKSNGMLVRILTSDILYAEAMA
                     DYVNIHTADARHTVHSTMKSLQDSLPEENFFRVHNSFIINLDRITRIEDNLVIIDKKP
                     IPVSRSRIRPLMQRLNTIS"
     gene            2943..3410
                     /locus_tag="VFU15_03950"
     CDS             2943..3410
                     /locus_tag="VFU15_03950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716955.1"
                     /translation="MKNPVTMDRFVFAGKHPAVFTGLVTAGGLIAYFLVMRAAGLVQV
                     TELRLFNFVILFAGVWWSMKRETAGAEKDHSYFSTLGNGCAVVITAVAFFSLFILFYL
                     NFDNEAMEQIREHAAAGRILTPEMAAIAVFAEGLSSGIVMAYVFLSYLRLKQN"
     gene            3457..3822
                     /locus_tag="VFU15_03955"
     CDS             3457..3822
                     /locus_tag="VFU15_03955"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU4716956.1"
                     /translation="MNILLVDDDAMITELLSMKLGEKGYNVEQVSNGWDALKELSTQK
                     YDLLITDLMMPDISGLTVISLLKNYVFGKIPVIIITSLDQSTMVLSGMGLGASDYFVK
                     PINVEKLLNRIRKLTAGKA"
     gene            3859..4755
                     /locus_tag="VFU15_03960"
     CDS             3859..4755
                     /locus_tag="VFU15_03960"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEU4716957.1"
                     /translation="MKMNVRNILVPVDFSETSRKALAHASFLARLTKSHMDIIHVLPV
                     SPYYLEIPEPMLYVNNHSEVNQLVGGKLEEFSDEIHGIYGIRPRHILAHGQVAYEIVK
                     AAREGKADLVIMGTHGAKGFEELFIGSNAHKVVTLAPCPVITIQEHAGNPGFRNIVLP
                     IDRSRHSREKVETALSFALLYQSVIHILGLLDGDDENEYGKLQIVLDQVQHAVEHAGL
                     AFSRHTIRGGNLASEALKYGISVNADLIIILTDHESALTGIFPGPLARQIVNHSRIPV
                     MSLKPHYGSFETLDLSGAYSAY"
BASE COUNT         1259 a         1272 c         1319 g         1035 t
ORIGIN      
        1 acggtccgct gccgtcttgt ttcactgcag gataaggaag cggtgctgga attttccgtg
       61 caggataccg gcatcggcat tgcgcccgac aagatcgaaa aggtgttcga aagcttcacg
      121 caggccagct ccgacacgaa ccgcaaattc ggcgggaccg gtctcggcct gacgatctcg
      181 cgccagctcg tggagctcat gggcggaaag ctcaccgcac gcagcacgcc cggcgaagga
      241 acgacgttca cgttcactgt ttcattgccg gtcggtgagg gcggaacgcg gcaggcgacg
      301 gaagaagtgc tgcagctgga catgatccgc cgcgcaaagc tgctgctcgt cgaggacaac
      361 gagttcaacc gcatcgttgc cgtcgacacg ctgaacgaat ttctcggtga agtgcaggtc
      421 gatgttgcgc gtgacggaaa tgaggcgctc gggaagatcg cgcagaacag ctacgatctc
      481 gtgctgatgg atatacagat gccgggcatg gatggctatg acgcaacgcg gcacatccgg
      541 aagatggctt caccgttgaa taaaattcct attcttgcaa tgacggcaaa cgccacgccg
      601 gaagagatcg cgaaatgcag cgaatgcgga atggacgggc acgtggcgaa accgtttgtt
      661 ccggaagcgt tgttccgcaa aatgtgcgag ctgctgaatg cccgggatgc gaatgcgtaa
      721 cacagccata acattatcct gatgagcgac gaaaacaaaa cgcagaccga cctcgggttc
      781 ctgggaacgt ttaccgggaa cgatacggcg cgcatgcgca aatacatcac cctgttcctc
      841 ggcgccgcac ctgcggaagc cgaagcgatc cgcagcgcgc tgaccaccgc cgatcacgag
      901 aaagtgcgtg cgtctgcaca cagcctgcgg ccgcagatga cctacatggg cgcgaaagcg
      961 ggggaggagc tgctgaaacg catggaagag gatgtgaaag caggaaagta cgatgagctg
     1021 caggtgacgg gcgcgaagtt cgaggcggtt ttcagcaagg tctgcgagga actgcaggcg
     1081 taccttaaac aaatcagtta aatttaaccg ttcaaaaact gaccgtaatg aaagaagtaa
     1141 acaccatttt catcgttgac gacgatgcca cgcagcttac gatggttgcc gaccaccttt
     1201 ccaaattcaa gaacattacg atcaagacat tttcttcggg agaagaatgc atcaaaaacc
     1261 tttcgctgaa gccgcaggtc attttcctcg attataattt cgacaaggcc ggcgacaaag
     1321 cgaagaacgg catcgacgtg ctgaaatcca tcaaggatgc ggatccttcc atcgaggtgg
     1381 tcatgctttc cgggcaggac aggatcgagg tggccgtcaa cacgatgaaa tacggcgcgt
     1441 tcgattacat cgtcaagaat gaaagctctt tccaccgcgc ggagaacgcg gtaaaaaata
     1501 tcatccgcaa gatcaggctc gaaggaaacg taaaaatcta caagcgcctg ttcatcacgt
     1561 tcgcgatctg tttcacactg atgatcgcaa ttattattta cctgtgggcg aacggataca
     1621 tcgcaaaaaa tcccggctgg ttctgatcgt cagcgggaaa tcatgcaaca ggccggccat
     1681 tcgccggctt ttttgttttc gttccgggta aaaacgggac agcgtacaat gaaaaacgcg
     1741 caataaaaaa aacgggaacg aagctgaccg acttcgctcc cgtgcggtgc ataacgctta
     1801 tcaaagaacg gacacccctg cgtgtgaaat tgaaaggaaa ccgaaaatta agcgcaccgt
     1861 ctgccgttcg taaaacgtca tctgttttac ttaccgttac cgtcaaacaa gaagcggtaa
     1921 ctgcatcaaa tgtaaaaagg caatttgcgg ggaaggacga tttttagatt cccgggaaga
     1981 ttccggcgac gccctgcaaa aaaacgggga tttcagggaa cttaatcgcc tgcctttcgt
     2041 tgaataaaac acgtacctta ttcagagaat actatgagca caaaatccgc atccctgaat
     2101 tgcctcgtgg tcgatgacga tgacatggtg cgcatcgaca tcgggcagaa gatcagcgac
     2161 acgccttttc tccacctggc cggatcgtgc gcttccgccc tggaagccgc tgctgtgatg
     2221 cagaaggaaa agatcgacct ggttttcctg gatatcctga tgccggaaat gacaggactc
     2281 cagttcatgg aaagcatggg cgaaaaaagc atccaggtca ttttcatcac gtcggaaaaa
     2341 gattacgcgg cacaggcgtt cgatctcgac gtcacggatt ttctcgtcaa gccggtttcc
     2401 atggaacgct ttctcaaggc cgtcatgcgc gcatcgtcga atgtgaagac aaccggcggg
     2461 gatgcggtga gaaacaatca tctttttgtg aagtcgaacg gcatgctcgt ccgtatactc
     2521 accagcgaca tcctctacgc ggaagcgatg gcggactacg tcaacatcca taccgctgat
     2581 gcgcgccaca ccgtgcattc gacgatgaaa tcgctgcagg attcgctgcc ggaagaaaat
     2641 tttttccgcg tgcacaattc atttatcatc aacctcgacc gcatcacgcg catcgaggac
     2701 aacctcgtga tcatcgacaa aaagccgatc cctgtcagcc gctcgcgcat acgcccgctc
     2761 atgcaacggc tcaacacgat ctcctgagca ccggcttttt catttccccg accgacggtc
     2821 gaaagagggc ggcaaagtat cgaagttgcc gcaccgccgc ccatttccct gcgtaaacat
     2881 taccggaaga aaagttccgg gcaggtgcag tgtataagct gcatttcccg ggcatgacac
     2941 acatgaaaaa tccggtaacc atggatcgtt ttgtttttgc aggaaagcat cctgcggttt
     3001 tcacgggatt ggtcacagcg ggaggactta ttgcctattt cctcgttatg cgggcggccg
     3061 ggctcgtaca ggtaacagag cttcgcctct tcaattttgt tattcttttc gccggcgtat
     3121 ggtggtcgat gaaaagagaa acagcaggtg cggaaaaaga tcacagctat ttcagtacgc
     3181 tgggtaacgg gtgcgcggta gtgatcaccg cggtggcttt cttctcgctc ttcatcctct
     3241 tttacctgaa tttcgacaat gaagcgatgg agcagatcag ggaacatgca gcagcggggc
     3301 ggatccttac acccgaaatg gcggctatcg cggttttcgc ggaaggtctt tcttcgggaa
     3361 ttgtcatggc ctatgtattt ctttcctacc tgcggctgaa gcagaattga tttcccggga
     3421 aaatgaaagt ggataaaaaa acgaaaggag agaacgatga atattcttct ggtagacgac
     3481 gatgcgatga tcaccgaatt gctttccatg aagctgggtg aaaaaggata caatgtcgaa
     3541 caggtatcga acggatggga tgcgctgaag gaattgtcca cgcagaaata cgacctgctg
     3601 atcaccgacc tgatgatgcc cgacatttct ggactcaccg tgatcagcct gctgaaaaat
     3661 tatgtcttcg gaaaaattcc cgtcatcatc attaccagcc tcgaccaatc gacgatggtg
     3721 ctttccggga tgggtctcgg cgcttccgat tatttcgtga aaccgatcaa cgtcgagaaa
     3781 ctgctgaacc gcatacggaa gctcacggcc ggaaaagcgt gagtttattt cgtacacgac
     3841 acactaaaaa cgccgcttat gaaaatgaac gtccgaaaca tcctggtgcc ggtcgatttt
     3901 tccgaaacca gccgcaaagc gctggcgcac gcgtcattcc ttgcgcgact gacgaaatcg
     3961 cacatggata ttatccacgt gctgccggta agtccctatt atctcgagat cccggaaccg
     4021 atgctgtacg tgaacaacca cagcgaggtg aaccagctcg tcggcggcaa gctcgaagaa
     4081 ttttccgacg agatacacgg gatatacggc atacggccgc ggcacattct tgcgcacggg
     4141 caggtggcgt atgaaattgt aaaagcggct cgtgaaggaa aggcggacct cgtcatcatg
     4201 ggaacacacg gtgcgaaagg atttgaagag ctgttcatcg ggagcaatgc gcacaaagtg
     4261 gtaacgctcg cgccctgtcc cgtgatcacg atccaggaac atgcgggcaa cccgggattc
     4321 cgcaacatcg tgcttcccat cgaccgttcg cggcattcgc gcgagaaagt ggaaacggcg
     4381 ctttccttcg cgttgctgta ccagtcggtg attcacattc tcggcctgct tgacggtgat
     4441 gacgaaaatg aatacgggaa gctgcagatc gttctcgacc aggtgcagca cgccgtggaa
     4501 catgcgggac ttgcattcag ccgccacacg atcaggggag gaaatctcgc gtcggaagcg
     4561 ttgaagtacg gcatcagcgt caatgccgat ctgatcatca tcctcaccga tcacgaatct
     4621 gcgctcaccg gaatttttcc cggcccgctg gcgcggcaga tcgtcaatca ttcccgcata
     4681 ccggtgatga gcctcaagcc gcattacggc agcttcgaga cgctggatct ctccggcgcc
     4741 tattcggcct attgagaatt ccggtacagt ctttcacttt cgtggaaacc gggagcgtga
     4801 ttttttcgcg aagccggtga aggtttaatg cgccccgcgc gaaacgcgaa cgttaccgaa
     4861 acgcgggtaa taattatatt tgaga
//
LOCUS       DASTSF010000135         4881 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1703951,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000135 DASTSF010000000
VERSION     DASTSF010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4881
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1703951"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..439
                     /locus_tag="VFU15_03965"
     CDS             <1..439
                     /locus_tag="VFU15_03965"
                     /inference="COORDINATES: protein motif:HMM:NF039201.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEU4716958.1"
                     /translation="VIQQPLQVQYKWLASSMKIPVLEVDASVINSNEVVTGVTYQDSL
                     RSNLFQVGVQDPAAGFARAGIFPNPAVDHTDVQLFSAQESEVDITLYDNSGRMVRNYG
                     TRSLGSGETVITLDLSGLAAGIYKLQVAGRGQLYCGKIAICGG"
     gene            676..>4881
                     /locus_tag="VFU15_03970"
     CDS             676..>4881
                     /locus_tag="VFU15_03970"
                     /inference="COORDINATES: protein motif:HMM:NF040138.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HEU4716959.1"
                     /translation="MKRIITLLGLLAVFSPAFAQNDLSVTAITQPVSGCALTSTENVT
                     IRIFNFGNTLITGTSFNVSYTINAGAPVVELVTLASPLLSNSTLNYTFTTQANLSVPG
                     TYTFDASVALAGDINPTNDAFTGYSVVNTPSSVGGSVSGGTDVCVSGNAGVLTLSGET
                     GSVVRWEYSTDGGVTWINISNTTNTQSYSNLTVPTQYRAQVQSGSCATATSSIASMTI
                     DPVSVGGTVNSNATVCSGANAGTLTLTGKTGSVVRWEKSTDGGVTWVNIANTTTSQSY
                     LNLVTTTKYRALVQSGACSSVHSSIATITVSPTTVGGTLNPASTTVCAGSNSGTLTLT
                     GKVGSVVRWEHSNDGGVTWLNIANTTTTLTYTNITQTNLYRVRVQSSPCSVQYSTVDT
                     VNVTTASVGGSLAPASSTVCSGMNHDTLVLSGESGSIVQWEESTDGGTTWTVIANTTN
                     TEIDSNLTMTTMYRALVQSGGCSASYSSTATINVDAVTVGGTVSGGVTVCSGSNGGTM
                     MLNGQSGSILQWEFSNDQLTWNPIANTTTSQNFSNLTDTTYYRVIVQSGVCPADTATQ
                     DTVIVDPVSVGGTISPAADTVCSGINSGTLTLVGDTGSVVQWEYSTDNGVTWITISNT
                     TTSQSFNNLTTATIYRALVKSGVCSQTYSAQATVSMNQQATGGTLYSDATVCGGSNNG
                     TLTLVGFNGSVTQWESSIDNGVTWSAIPNTNYTYGYLNLVDTTKFRVIVASGVCPTDT
                     SSIVTISVDPPSVGGTLSMDDTVCAGSNAGTLSLSGQTGSVIGWEYSTDGGVTWVNIA
                     NTTTTQNYVNLAQTTMYRVDVGNGVCAPVTSSVATITVDPPVNGGMVTGSTTVCASGN
                     SGSLTLSGSSGTIVTWETSTDGGATWVADGNTTTTENYTNLTDTTWYRAILQSGTCGG
                     DTSTVGVITVDPVPVGGTTSPNALACAGMNNGVIHLTGDTGSVSRWEYSTDGGNNWIA
                     LSNTTDSLTYVNLTMTTMYRAVVMSGVCNPPVYSSNDTITVSPMTVPGVVTGSAQVCE
                     GTGSGVLTLTGYTGSILGWETSSDGGMSWSPVPNATPTQAWSNPVDTTWYHVLVTSGA
                     CPLDTSTVAVVSIYPKPVAGFTTVPVCFGSPTAFTNTTTISSGGIQFQSWDFGDNGAS
                     VASNPVYTYGTSGSFNATLIVVSDNGCSDTATNAVVVNALPSSAITASGSTTLCSGDS
                     VMLSVPSSPQDHYLWTNGSTTDFIYAMAAGTYGVTVTDTVTSCMSMDSAMISVLPRPV
                     ASAGPDTSVSAGSSITLLGSGGDFYSWSPTVGLSDPNVANPACTPPYTVTYTLTVTAQ
                     NGCTDTDAVAVTFVKDYNIIISNLITANGDGYNDVWNIQNIEFYPNNKVTIYNRNGMV
                     VYEAEGYNNSWNGTYNGQQLPDGT"
BASE COUNT         1128 a         1309 c         1305 g         1139 t
ORIGIN      
        1 tgtcattcag cagccgctgc aggtgcagta caaatggctg gcttcctcca tgaagatccc
       61 cgtgctggaa gtggatgcgt cggtgatcaa cagcaacgag gtggttacgg gcgttacgta
      121 ccaggacagc ctgaggagca atcttttcca ggtgggcgtg caggacccgg cggcaggttt
      181 cgcccgggct ggcattttcc cgaacccggc cgttgatcat accgatgtgc agcttttttc
      241 cgcacaggaa tccgaggtgg atattacctt atatgataat tcgggcagga tggtcaggaa
      301 ttacggcacc aggagcctgg gttccggtga aaccgttatc acgcttgacc tttcagggct
      361 tgcggccgga atttacaaat tgcaggtggc gggccggggc cagctgtatt gcggtaaaat
      421 agcaatatgt gggggctaat aatctgcttt tcaaattgtt attctaataa gctttttgta
      481 accatttaat ggacacagga gtataaaagg gcgtatgcct gaaaagcata tcacaccaga
      541 atgcatcagt ttttgaaaaa attatccggg tttttcttcc caggctttcg gcttatgccg
      601 gcccggatca gttgcgttcg ctctttgtct cccacgacaa aaaacaaatt catttaaacg
      661 agaaattttc agcttatgaa gcgaatcatt actcttctgg gtttattggc agttttttca
      721 ccggcattcg cgcagaatga tctttcggtg acggccatta ctcaacccgt aagcggatgc
      781 gccctgacat ccaccgaaaa cgtcacgata cggatattca atttcggaaa cacgcttatt
      841 acgggcacct cgtttaacgt cagctatacc atcaatgccg gggcgccggt ggttgagctc
      901 gtgacgctgg cttctccgct tctttcgaat tccactctca attatacgtt taccacccag
      961 gccaaccttt ccgttccggg cacatatacg ttcgatgctt ccgttgcact ggcgggtgac
     1021 atcaatccca ccaatgatgc gtttacgggt tattcggttg tcaacacgcc ttcctctgtc
     1081 ggtggcagtg tgagcggcgg aactgatgtc tgtgtttcgg ggaatgcagg tgtgctgaca
     1141 ctttccggtg aaacgggcag tgtggttcgc tgggaatatt ccacggacgg cggcgtaaca
     1201 tggatcaata tttccaatac gaccaacacg cagtcttaca gcaacctcac cgttcctacg
     1261 cagtaccgtg cacaggtgca gagcggaagc tgcgccacag ccacttcttc catcgcatcc
     1321 atgacgatcg atcccgtctc ggtgggagga acggtcaaca gcaatgccac ggtctgttca
     1381 ggcgctaacg caggtacgct tacattgacg ggtaaaaccg gttccgtggt gaggtgggaa
     1441 aagtctactg acggcggagt tacctgggtc aatattgcga atacaaccac ttcccagtcg
     1501 tatctcaatc ttgttaccac aacaaagtac agggcgcttg ttcagagcgg cgcctgttca
     1561 tccgttcatt cgagcatcgc cacgatcacg gtaagcccga ccacggtagg tggaacactg
     1621 aatcctgcga gcacgactgt gtgcgccgga agcaacagcg gcacgctgac gctgaccggt
     1681 aaagtgggct ctgttgttcg ctgggaacat tcgaacgatg gtggcgtcac gtggctgaat
     1741 attgcgaata ccaccaccac gctcacctat acgaacatta cgcagactaa tttgtaccgc
     1801 gtccgtgtgc agagcagccc ctgttcggtt cagtattcta ccgtagacac cgttaatgta
     1861 acaactgcgt ccgttggcgg ttctcttgct cctgcatctt caacggtttg cagcgggatg
     1921 aatcacgata cgctcgtgct gagcggcgaa agcggatcca tagtgcagtg ggaagagtcc
     1981 acagacggcg gcactacatg gacggtcatt gcaaacacga ccaatacgga aatagattcc
     2041 aaccttacca tgacgacaat gtaccgcgct cttgtgcaga gtggcgggtg ttcggcttcc
     2101 tattcttcaa ccgcaacgat caacgtggat gctgttacgg tcggtggcac cgtttccggt
     2161 ggcgtgacgg tctgcagcgg cagcaatgga ggtacaatga tgcttaacgg gcaatccggt
     2221 tccattctgc agtgggaatt ttcaaatgac cagttgacct ggaacccgat cgcgaatacg
     2281 acgacctccc agaatttcag caatcttaca gacacgacgt attaccgtgt tatcgtgcag
     2341 agcggcgttt gtcctgcgga tacggccacg caggatacgg ttattgtaga tcctgtttcg
     2401 gtggggggaa ccatttctcc tgcagcggat actgtctgca gcgggatcaa cagcggaacg
     2461 ctcacgctgg tgggtgatac gggcagcgtg gttcagtggg aatattctac ggataacggc
     2521 gtaacgtgga tcaccatttc caatacgaca acatcgcaat cgttcaacaa cctgacgacc
     2581 gcaaccattt accgtgcgct ggtgaaaagc ggcgtctgct cacagactta ttctgcccag
     2641 gctaccgtat caatgaacca gcaggccaca ggcggaacgc tgtattccga tgcgacggtt
     2701 tgtggcggaa gcaataacgg cacgcttacg ctggttggct tcaatggatc tgttacccaa
     2761 tgggaatctt ccatcgataa cggagtaacc tggtcggcga tccccaatac aaattatacg
     2821 tatggttacc tgaaccttgt ggacacgacc aaattccgcg tcatcgtggc aagcggcgtt
     2881 tgtcccaccg atacttcatc gattgttacc ataagtgttg atcctccttc tgtaggtgga
     2941 acgctttcaa tggacgatac cgtttgtgcg ggcagcaacg cgggaacgct ttcactctcc
     3001 ggccagacag gcagcgtgat cggatgggaa tattctacag acggcggtgt gacgtgggtc
     3061 aacatcgcaa atacaaccac gacgcagaac tacgtcaacc ttgcacagac aaccatgtac
     3121 cgagtcgatg tgggcaacgg tgtttgtgcg ccggtcactt cttccgtggc aacgatcacg
     3181 gttgatcctc ctgttaacgg tggaatggtt accggaagca cgacggtatg cgcttcgggt
     3241 aattccggct cgcttacatt aagcggatct tccggtacga tcgtcacctg ggaaacgtca
     3301 acagacggtg gagcgacctg ggttgctgac ggtaatacaa caacaacaga aaactatacc
     3361 aacctcacgg atacgacatg gtaccgtgca atcctgcaaa gcgggacgtg cggaggtgac
     3421 acatcgaccg taggtgtgat cacggtcgat cctgttcctg tcggcggaac gacttctccg
     3481 aatgcgcttg cctgtgccgg catgaacaat ggcgttattc accttaccgg ggataccggc
     3541 agcgtttcac gatgggaata ttccaccgac ggaggaaaca actggatcgc gctttccaat
     3601 acgacggatt cgctcacgta tgttaatctt acgatgacga caatgtaccg tgccgtcgtg
     3661 atgagtggtg tctgcaatcc cccggtctat tcttccaatg atacgatcac cgtttcgccg
     3721 atgactgttc ccggtgtcgt caccggaagc gcgcaggtat gcgaaggaac cgggagcggg
     3781 gtgcttacac tcacaggata tacgggcagc attctcggct gggaaacttc ttccgacgga
     3841 ggaatgagct ggtcgccggt cccgaatgcc acaccgaccc aggcatggag caatcctgtc
     3901 gacaccacgt ggtatcatgt gctcgttacc agcggcgctt gcccgctgga tacttcaacg
     3961 gttgccgtgg tttccattta tcccaaaccg gttgccgggt ttacaacggt tcccgtctgc
     4021 ttcggttcac ccactgcatt taccaacacg acaacgatct cgtccggcgg catccagttc
     4081 caaagctggg atttcggtga caacggtgct tccgttgctt cgaacccggt ttatacttac
     4141 ggaacatccg gttcattcaa tgctacgctc attgttgtgt cggacaacgg ttgctccgac
     4201 accgcaacga atgccgtagt ggtgaacgca cttccttctt cagcgatcac cgcttccgga
     4261 agcacaacgc tctgctcggg tgacagcgtg atgctttctg ttccgtcaag cccgcaggat
     4321 cattacctgt ggacgaacgg aagcacaacg gatttcattt atgcgatggc agccggaacg
     4381 tatggtgtca ctgtaacgga tacggtcact tcgtgcatgt cgatggattc ggcaatgatc
     4441 agcgtgctgc cgcggccggt ggccagcgca ggacctgaca ccagcgtcag cgcaggatct
     4501 tccatcacgc tcctcggcag cggcggagat ttctacagct ggtcgccgac ggtcggcctc
     4561 agtgatccga atgttgccaa cccggcctgc acacctccgt atacagtcac ttacacgctg
     4621 accgtcaccg cgcagaacgg ttgtacggat acggacgcgg ttgccgtaac gttcgtgaaa
     4681 gattacaaca tcatcatttc gaatcttatc acggccaatg gtgacgggta caacgacgtc
     4741 tggaacatac agaacatcga attctacccg aacaacaaag tcacgatcta caaccggaac
     4801 ggcatggtcg tgtatgaagc ggagggctac aacaacagtt ggaacggcac ttacaacggc
     4861 cagcagctgc ccgacggaac g
//
LOCUS       DASTSF010000136         8832 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_587408,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000136 DASTSF010000000
VERSION     DASTSF010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 8832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8832
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_587408"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1283
                     /locus_tag="VFU15_03975"
     CDS             <1..1283
                     /locus_tag="VFU15_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015806185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HEU4716960.1"
                     /translation="GIIMAIGQFLLFGSATLLSSNIGLAKIIFFAGLLALIIGNGFFK
                     PNISTMVNQLYPVGDKRVDAAFSIFYMGINLGAFFSPLVCGTLGEKIDYKWGFLAAGI
                     GMVIGVLVFEMLKNKYIVSAEGAPVGMKPEKKLHITEEGKEDLGEKEFSPARILLWVG
                     VYAALWIIFMFVVHFNFVSTLIFSTSLAAAGFVITDPTLTKVERDRIIVIYIIAFFVI
                     FFWSAFEQAGASLTLFADQQTDRTIFGWEMPASYFQSINPLAIIFLAPIFAIIWTSLG
                     KRNIEPPSPIKQALGLFLLAFGYLIIAFGVHGVNGTAKVSMFWLFSMYVIHTMGELCL
                     SPIGLSMVARLSPLRLGSLLMGIWFMSNAMANDFAGMLSALYPENGKVTHLFGFEITG
                     LYDFFMVFVVMAGVASLLLFLLSKKLRTMMHGIR"
     gene            1378..3450
                     /locus_tag="VFU15_03980"
     CDS             1378..3450
                     /locus_tag="VFU15_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014680515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HEU4716961.1"
                     /translation="MEENNAFKPYVPDEAKMPELTLRAILLGCIFGILFGAANVYLAL
                     NAGLTVSASIPIAVLAISIGRKFLKTTILENNIIQTTGSAGESIASGVVFTLPAFLFL
                     SAEASGEKYFNYFTILALAIIGGMLGVLMMIPLRRSLIVKEHGKLPYPEGTACAQVLI
                     AGDKGGNFAKTAYLGLGAATIYAILQRMFHIIAVTPRLLIAQTNKFLPSARVDGDVTP
                     EYLGVGYIIGPRIAGQLVAGGVLAWLALIPLLAFLLHDHMDIIAAQQVKLGLLSSISD
                     AGGAGGWNPVTHTFSDTAEAIYRAYIRQIGAGAVAAGGFITLFKTLPTIISSFREGLT
                     SIRNRESSTTRKRTERDLSLKVTLIGCLALVIVIALLPNIPASGIGGRILTGILVIVF
                     GFFFVTVSSRIVGIIGSSNSPVSGMTIATIMGTSLVFIAFGFSGKVFEPMVLVIGSLI
                     CIAAANAGNTSQDLKTGFLIGATPRSQQIALFTGAIVSTFVVGFVVHLLDTPTPEMVQ
                     QGIHHAIGTKFSAPQGTLMATLIRGLLSFNLDWQFVMVGVLLAVTMELCGVNSLSFAV
                     GAYLPLATTLPIFAGGCVKGIVDWRAKRKGEKAEDAELGRGSLFATGLVAGGALMGVI
                     FACLSAFPSSRSFLDGLILGKSLGETDPDDGIGNGVYSLIAVCFFVALAFVLYRVATK
                     KGSGPKVA"
     gene            3481..4089
                     /locus_tag="VFU15_03985"
     CDS             3481..4089
                     /locus_tag="VFU15_03985"
                     /inference="COORDINATES: protein motif:HMM:NF024498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF255 domain-containing protein"
                     /protein_id="HEU4716962.1"
                     /translation="MKKILIAGIIALPLLYWAGVRYSRAQNAPAATMPTEIKWYTLEE
                     AQKLDDSIPRKIFLDISTSWCGWCKVMEANTFKNPIIAKYMNEHYYPVKVDAESHDTL
                     VFNGQKFWNRGPANMRSANDFAITVLQGHLSYPSFAFISKDRKTFTIMQGYMPPEQFE
                     PYLHYYGEDKETVMSWDDFLKTFKSELPPPDSTKLMQAPAPH"
     gene            4104..4508
                     /locus_tag="VFU15_03990"
     CDS             4104..4508
                     /locus_tag="VFU15_03990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716963.1"
                     /translation="MEMETEHTLHNLKERIVAVLSARNLTYAQLVEYLGVSEAALDKA
                     LADNTIEIRTLEQISKELRIPLYSFFRDPDYQPGDENEQPFYNVNIWAPKEIHLLTEN
                     DNLRQEIERLRLEIAKKELLIQGLEQQLKKEG"
     gene            complement(4509..5267)
                     /locus_tag="VFU15_03995"
     CDS             complement(4509..5267)
                     /locus_tag="VFU15_03995"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEU4716964.1"
                     /translation="MVFDAIIVDDEPKSREVLRALISRYCPNVKIVGLAANIVDAKNL
                     ISKFSPSLVFLDVEMPGGNGFRLLDEVDRKKFDIIFVTSYGHYAIPALRYSAIDYLLK
                     PVEIEELREAVQRVVQKRNSLSDDGENYKVLSGNLKAPVSLQKIAIHGLNDVKFIPVA
                     EIIRMEADNNYTFIITFSGDRYLASRTMKDFEEMLSGMRNFFRVHKTHLVNLNHIRKF
                     VKGDSPYIVMSDGSNIEVSRRKKQELIERMGVNS"
     gene            complement(5284..8277)
                     /locus_tag="VFU15_04000"
     CDS             complement(5284..8277)
                     /locus_tag="VFU15_04000"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HEU4716965.1"
                     /translation="MCCRFLSRVFFFSVLLNLFPFLPVRAQPLVVPGGFHHIAVKDGL
                     PSSEVYKIFQDSKGYIWMLTDAGVCRYNGYVYSSFTSQDGLTDNTVFRIAEDNTGRIW
                     LQGFSGALNYYEDGAFHQVPADDSLRKVYLQGQRTGYCMALDRYNGVTIGGLYTRQCY
                     RVSPADGYTHLQIDSGEGPRWVRRKTWVNEEGNIISYACDSFLVEDRGVFYLNHKRIL
                     VNFEPTIDNTTNNRICYSSKGLLYYSFGEWLYCIHPDGNVGKVRMSSMITGIDEDKEG
                     NIWVSLDRNGTFRFGNGDLSGKRTCYLPGKTVSSVLEDNEGAFWFSTVGQGVFYLPEI
                     NFHYLTRQEGLPGNAVLSSCALGADKVVFGFPETELVMIRSCGNDSFPFKSRRIAPGN
                     YLGYEVLLPMHDSILVNDSGMRVLDSDLNTVSFHRPYGHCKGGAISPVDGSAVMFSHS
                     MLFWVQRTGAVDFSMLVPLRFTCVCGSKDGTFWFGSLQGLWRSRGRSVYPMTDSVPEI
                     NMRIDKLQEDKAGNLWIATRGEGLFVLSGRKVIHFTEESGLASNSCRTLCVDSMQNVW
                     VGTNRGLTRISGFDPVTGHAVLRSFNSTNGLLSDEVTSLVFNCGKIWIGSYEGICWVD
                     PDLLDGGNYSPPVYIKKILYGDSSISVKGIPEFAFGENAVRIFLEGLYFRDTKHLQYE
                     YRLSGVDDDWIVTSNTEISFSGLAPGEYTLQVYAVSPNNVVSKTPAVFSFRVLPPYWM
                     KWWFITFEVLLLAAVIYFVVSYRLKKQRQREQEKNETEKRIAELRLIALRSQMNPHFI
                     FNAINSIQHFVLQNDSEQAYTYLSKFSRLIRLVLDQSQSEKITLAQELSILRLYVELE
                     QLRFERPFEFFLDVDPVLLDSNICIQGMLVQPFVENAIWHGLIPKKEGNAYVKVVFRK
                     ENSFLCISIEDNGVGRSQSTKAGEENVGRKKSYGMMITRERLSLSEKNPSGQPRFIIT
                     DLVDENGKPAGTRVEIRVELDEDDF"
     gene            complement(8292..8612)
                     /locus_tag="VFU15_04005"
     CDS             complement(8292..8612)
                     /locus_tag="VFU15_04005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716966.1"
                     /translation="MSARLFFFFAFYLLLTGNVPAAISKPDSLKGDTSTVWLDTVNRS
                     IVVEEEYVYVEVLDGAGRLVTSSKNTFRQMRIDMKGKPAGAYTVKVKNNRLDYSRTII
                     LPEE"
BASE COUNT         1992 a         2462 c         2288 g         2090 t
ORIGIN      
        1 ggggtattat aatggctatc gggcaattcc tgcttttcgg aagcgccaca cttctttctt
       61 cgaatatcgg gcttgccaag atcatcttct ttgccggcct tctcgcgctc attatcggta
      121 acggtttctt caagccgaat atttccacga tggtcaacca gctttatccc gtaggtgata
      181 agcgtgttga cgctgcgttt tccatttttt acatgggcat caacctgggt gcattcttct
      241 ccccgctggt ctgcggaacg cttggcgaaa agatcgatta caaatgggga ttccttgccg
      301 cgggtatagg catggtgatc ggggtgcttg tttttgaaat gctgaagaac aagtacatcg
      361 tctccgctga aggtgcgccc gtgggtatga agccggaaaa aaagcttcat atcacggaag
      421 aaggaaagga agatctcggg gaaaaagaat tttcgccggc acgtatcctt ttgtgggtcg
      481 gcgtttacgc ggcactgtgg atcattttca tgttcgtggt tcatttcaat tttgtcagca
      541 cgctcatatt ttccacttcc ctggccgccg ccgggtttgt catcaccgat ccgacgctga
      601 ctaaagtgga gcgggaccgt atcattgtca tctacatcat cgcattcttc gtcatctttt
      661 tctggtcggc gtttgagcag gcgggcgctt cgctcacttt gtttgcagac cagcaaacgg
      721 accgtactat tttcgggtgg gaaatgcccg ccagctattt ccagtccatc aacccgctgg
      781 ccatcatttt cctggctccc atcttcgcca ttatctggac atcgctcggc aaacgcaaca
      841 ttgagccgcc ctcgccgatc aagcaggcgc tcggactttt cctcctggcc tttggttacc
      901 tgatcatcgc tttcggcgtt catggcgtca acggaaccgc gaaagtgagc atgttctggc
      961 tcttcagcat gtatgtcatt cacacgatgg gcgagctttg cctttcaccg atcgggcttt
     1021 ccatggtggc gcggctttcc ccgctgcgcc tcggttcgct gctgatgggg atctggttca
     1081 tgagcaacgc catggcaaac gatttcgccg gcatgctgag cgcgctctat cctgaaaacg
     1141 ggaaagtgac gcacctgttc ggcttcgaga tcaccgggct gtacgatttc ttcatggtgt
     1201 tcgttgtaat ggcgggcgta gcttcgctcc tgcttttcct gctgagcaaa aagctgcgca
     1261 cgatgatgca cggcatcagg taacaggaaa tcaaaaaata aaattcaaga tcccgggttc
     1321 ctgaacagca gaaagtgcga atggaatccg ggatctttcc tttaatgccg ctctgaaatg
     1381 gaagaaaaca acgctttcaa accctacgtt ccggacgaag cgaaaatgcc ggaactcacg
     1441 ctgcgggcca tattgctcgg ctgcattttc gggatactct tcggcgctgc gaacgtgtac
     1501 ctcgcgctca acgccggtct caccgtttcc gcttccattc ccatagcggt gctcgccatt
     1561 tccatcggca ggaaattcct gaagacgacc attctcgaga acaacatcat ccagacaacc
     1621 ggctccgcag gcgagtcgat cgcatcgggt gtcgtgttca cgctgcctgc gttcctgttc
     1681 ctcagcgcag aagcgagcgg ggaaaaatat ttcaattact tcaccattct cgcgctggcc
     1741 atcatcggcg gaatgctcgg tgtgctgatg atgatcccgc tgcggcgctc gctcatcgtg
     1801 aaagagcacg gaaagcttcc gtacccggaa ggaacggcct gcgcacaggt gctcatcgcc
     1861 ggcgacaaag gcgggaattt cgcgaagacc gcctacctcg gtcttggcgc cgctaccatt
     1921 tacgcaatcc tgcagcgcat gttccacatc atcgcggtga cgccgcggct tctcattgcg
     1981 cagacgaaca agttcctgcc ttccgcgcgg gtcgacggtg acgtgacgcc cgaatacctc
     2041 ggcgtaggtt acatcatcgg tccgcgcatt gccgggcagc tggtagcggg cggcgtgctt
     2101 gcctggctgg cgctcattcc gctgctcgct ttcctgctgc atgaccacat ggacatcatc
     2161 gcggcgcaac aggtgaagct gggcctgctc agcagcatca gcgacgcggg cggtgctggc
     2221 ggatggaatc ccgtcacgca tactttttcc gatacagcag aagcgatcta ccgcgcctac
     2281 atccggcaga tcggcgcagg cgcagttgca gccgggggct tcatcacgct tttcaaaaca
     2341 ttgcccacga tcatctcatc gttccgcgaa ggacttactt ccatccgcaa ccgggaatcg
     2401 tcaaccacgc gcaaacgcac cgagcgcgac ctctcgctta aagtgacgct catcggttgt
     2461 ctcgcgctcg tcattgtcat tgcattgctg ccgaacattc cggcctccgg catcggcgga
     2521 agaattctca ccggtattct cgtcatcgtg ttcggttttt tcttcgttac cgtatcgagc
     2581 cgcatcgtcg gcatcatcgg gtcgagcaac agtcctgttt cgggtatgac catcgccacg
     2641 atcatgggaa cctcgcttgt gttcattgcg ttcgggttca gcgggaaagt gttcgaaccg
     2701 atggtgctcg tcatcgggag cctcatctgc attgcggcgg cgaacgccgg gaatacgtcg
     2761 caggacctga aaaccgggtt tctcatcggc gcgactccgc gttcgcagca gatcgcgctg
     2821 ttcaccggcg ccatcgtatc cacgttcgtc gtcggttttg tcgtccacct gctcgacaca
     2881 ccgacaccgg aaatggtgca gcagggcatt caccatgcga tcggaacgaa gttcagcgcg
     2941 ccgcagggaa cattgatggc gacattgatc cggggattgc tttccttcaa tctcgactgg
     3001 cagttcgtga tggtgggcgt gctgctcgcc gtaacgatgg agctgtgcgg cgtgaactcg
     3061 ctttcgttcg cagtcggcgc ttaccttccc cttgcgacaa cgcttcccat tttcgccggc
     3121 ggatgcgtaa aaggaattgt cgactggaga gctaaacgca aaggagaaaa agcggaagac
     3181 gccgagctgg gcaggggaag tctttttgcg accgggctcg ttgcgggcgg agcgctcatg
     3241 ggcgtgatct tcgcctgcct ttccgcgttc ccttcttcgc gctcattcct tgacggtctt
     3301 atcctcggca aatcgctggg agaaaccgat ccggacgacg gcattggcaa tggcgtttat
     3361 tccctcatcg ccgtttgttt ctttgttgcg ctcgcttttg tcctgtaccg ggtggcaacg
     3421 aaaaaaggca gcggaccaaa agtggcctga ttttcgtaac ttcgttagta acgaattgac
     3481 atgaaaaaga tcctgattgc cggaattatc gctttaccgc tgctctactg ggccggcgta
     3541 cgttacagcc gtgcgcagaa cgcccctgcg gcgaccatgc ccacggaaat aaagtggtat
     3601 acgctggaag aggcgcagaa acttgacgac agcattccgc gtaaaatatt cctcgacatc
     3661 agcacaagct ggtgcggctg gtgcaaagtg atggaagcaa acacgttcaa aaatccgatc
     3721 attgcgaaat acatgaacga gcattactac ccggtaaagg tggatgcgga aagccacgat
     3781 acgcttgttt tcaacgggca gaaattctgg aaccgcggtc cggcgaacat gcgcagcgca
     3841 aatgattttg cgatcacggt tctccagggg cacctctcct atccttcgtt cgcattcatc
     3901 agcaaagacc ggaaaacatt caccatcatg cagggctaca tgcccccgga acagttcgaa
     3961 ccttatcttc actattacgg cgaagacaag gaaaccgtaa tgagctggga cgatttcctg
     4021 aaaacattca aaagcgaatt acctcctccc gattccacga aactaatgca ggcgcctgct
     4081 cctcactaac agaagacgtg cagatggaaa tggaaacgga acacaccctt cacaatctca
     4141 aggaaaggat cgtcgcggtg ctcagcgccc gcaacctgac gtacgcacag ctcgtcgaat
     4201 acctcggcgt ttcggaagct gcgctcgaca aggcgcttgc agacaacacg atcgagatcc
     4261 gcacgctcga gcagatatcg aaggagctgc gcatcccgct gtacagcttt ttccgcgatc
     4321 ccgattacca gcccggcgac gagaacgaac agcctttcta caatgtgaac atctgggcgc
     4381 cgaaggaaat tcatctcctc accgaaaacg acaatctccg ccaggagatc gaacggctca
     4441 ggctcgagat cgcgaaaaag gaattgctta tccagggtct cgagcagcag ctcaaaaaag
     4501 aaggctgatc aggaattcac gcccatccgt tcgataagct cctgcttttt ccgtcttgaa
     4561 acttcgatat tggatccgtc cgacatgacg atgtacggag aatcgccctt cacgaatttc
     4621 cggatatgat tgaggttgac gaggtgcgtt ttgtgcacgc ggaaaaaatt cctcatcccg
     4681 gaaagcatct cctcgaaatc tttcatcgtt cgcgaggcaa ggtacctgtc tcccgaaaat
     4741 gtgatgatga acgtgtaatt gttatccgct tccatgcgaa tgatctcggc gacgggaatg
     4801 aatttgacgt cgttgagccc atggatggcg atcttctgca gggaaacggg cgctttcagg
     4861 ttgccggaaa gaaccttgta gttttccccg tcgtcgctga gcgaattccg tttttgcacc
     4921 acgcgttgca ccgcttccct gagctcttcg atctccaccg gcttcagcag gtagtcgatc
     4981 gcgctgtaac gcaatgcggg aatagcgtaa tgaccgtaag acgtcacgaa aatgatgtcg
     5041 aattttttcc ggtccacttc atccagcagg cggaacccgt tgcccccggg catttcgacg
     5101 tcgagaaaaa cgagggaagg agagaatttc gaaatgaggt tcttcgcatc cacgatattc
     5161 gcggcaagcc ccacgatctt cacgttcggg cagtacctgc tgatcagcgc acggagcact
     5221 tcgcggcttt tcggttcatc atccacaatg atcgcgtcga acaccatgaa gtaaatttat
     5281 tatttaaaaa tcatcttcat ccagttcaac cctgatttcc accctcgttc ccgccggctt
     5341 cccgttctcg tcgacaagat cggtgatgat gaaccgcggc tgccctgaag gatttttttc
     5401 cgaaagcgaa agccgctcgc gcgtgatcat catgccgtac gattttttcc tgcccacatt
     5461 ttcttcgccc gctttcgtgg actggcttct tcccaccccg ttgtcttcaa tggaaatgca
     5521 gagaaagctg ttttcttttc tgaaaacaac cttcacgtag gcgttccctt cttttttcgg
     5581 tatcaatccg tgccagatcg cattttcgac aaaaggctga acaagcattc cctggatgca
     5641 gatgttcgaa tcgaggagaa ccgggtcgac atcgaggaaa aattcgaaag gacgttcgaa
     5701 acgcagctgc tccagctcca cgtacaggcg gagaatggaa agctcctgcg cgagcgtgat
     5761 cttttccgac tgcgactggt cgagcacaag gcggatgagg cgcgagaatt tcgagaggta
     5821 cgtatacgcc tgctcgctgt cgttctgcag cacgaagtgc tggatggaat tgatcgcgtt
     5881 gaaaataaaa tgcgggttca tctgcgagcg aagcgcgata aggcgaagct cggcgatccg
     5941 tttttcggtt tcattctttt cctgttcccg ctggcgctgt tttttcagcc ggtaggaaac
     6001 gacgaagtaa atgacagcgg ccagcaacag cacttcaaaa gtgatgaacc accatttcat
     6061 ccagtaaggc ggaagaacgc ggaaggaaaa aactgcgggt gttttgctca ccacgttatt
     6121 gggactcacg gcgtacacct ggagcgtata ttcgcccggc gcaagaccgg aaaagctgat
     6181 ctccgtgttc gaggtcacga tccagtcgtc atcaacgcct gaaagccggt attcgtattg
     6241 gaggtgcttc gtgtcgcgga aataaagtcc ttcgaggaaa atccttaccg cattttctcc
     6301 gaaggcgaat tccgggatgc ctttgacgga aatgctgctg tcgccgtaaa gtattttctt
     6361 gatgtaaacg ggcggcgaat aattgccgcc gtcgagaaga tccggatcta cccagcatat
     6421 tccttcatag ctgccgatcc atattttccc gcagttgaac acgagcgaag tgacctcgtc
     6481 cgaaagaagt ccgttcgtgg aattgaacga acgcagtacc gcatgtcccg tcaccgggtc
     6541 aaagcccgaa atgcgtgtca gtccgcggtt cgttcccacc cacacattct gcatggaatc
     6601 gacgcacagc gtgcggcagg aattactggc gagtccgctt tcttcagtaa aatgaatgac
     6661 cttccttcct gaaagaacga acaggccttc tcccctcgtg gcgatccaca ggttcccggc
     6721 cttgtcctcc tgcagcttgt cgatgcgcat attgatctcc ggcacggaat ctgtcatcgg
     6781 gtaaacactt cgtccccggc tccgccataa tccctgcagc gatccgaacc agaacgtccc
     6841 gtctttcgat ccgcagacgc acgtgaagcg cagcggtacg agcatcgaga aatcgacggc
     6901 tcccgttctt tgcacccaga agagcatgga atgcgagaac atcactgccg atccgtcgac
     6961 gggagatatt gcccctcctt tgcaatgccc gtaaggacgg tgaaagctga ccgtatttaa
     7021 atcggaatcc agcacgcgca tgccggaatc gttcaccagg atggaatcgt gcatcggaag
     7081 aagaacttcg taaccgagat agttgcccgg tgcaatgcga cgcgatttga acggaaaaga
     7141 gtcgttgccg cacgaccgga tcatgacgag ctccgtttcg gggaaaccga aaaccacttt
     7201 gtcggcgcct aatgcacagc ttgaaagaac agcgttaccc ggaagtcctt cctgccgcgt
     7261 caggtaatgg aagtttattt ccggcaggta aaaaacgcct tgtcccaccg tcgagaacca
     7321 gaacgcgcct tcgttatctt ccagcacgga gcttactgtt ttacccggca ggtagcatgt
     7381 ccttttcccg gaaaggtctc cattcccgaa ccggaatgtc ccgttacgat caagcgaaac
     7441 ccagatgttc ccttctttgt cctcgtcaat tcccgtgatc attgaagaca tgcgcacttt
     7501 cccgacattt ccatcgggat ggatacagta cagccattcg ccgaaagaat agtatagaag
     7561 tcccttcgag gaatagcaga tgcggttgtt ggtggtgtta tcaatggtag gctcgaagtt
     7621 caccaggatc cgcttatggt tgagataaaa tacaccgcgg tcctccacga gaaagctgtc
     7681 gcacgcgtag gagatgatat ttccttcttc attgacccat gttttgcggc gcacccatct
     7741 cgggccttct cccgaatcga tctgaaggtg cgtgtagccg tcggcaggag atacgcggta
     7801 gcactgacgc gtgtaaagcc cgcctatcgt aactccgtta tagcggtcca gcgccatgca
     7861 atagcccgta cgctgtccct gcaggtatac tttccgcagg ctgtcgtcgg ccgggacctg
     7921 gtggaacgcg ccatcttcat aatagttcag cgcgccggaa aacccctgca gccagatacg
     7981 tcccgtattg tcctctgcaa tgcggaaaac cgtattgtcg gtcagcccgt cctgcgaagt
     8041 gaatgaagaa tagacatagc cgttgtacct gcacacgccc gcatccgtca gcatccagat
     8101 gtatcctttg ctgtcctgga atatcttgta cacttcgctg ctcggcagtc cgtcctttac
     8161 ggcaatgtga tgaaagcccc cgggaacgac gagcggctgc gcacgcaccg gaaggaacgg
     8221 gaacaggttc agcaggacgc tgaaaaaaaa tacccgggat aaaaacctgc agcacataga
     8281 tcgggtcgcc gttattcttc gggaagaatt atggtgcgcg aataatcgag gcggttattc
     8341 ttcactttta cggtataggc tccggcaggc tttcctttca tgtcgatgcg catctggcgg
     8401 aaggtattct tcgacgaagt aacgagcctg cctgctccgt ccagcacttc cacataaaca
     8461 tattcttctt ccaccacgat gctgcggttt accgtatcca gccatactgt cgatgtatct
     8521 cccttcagcg aatccggttt agatatcgct gcgggaacgt tgccggtcaa caagaggtaa
     8581 aaagcgaaga aaaaaaacag cctggcgctc atcacggaat cttttctgaa agttaggaaa
     8641 gattcgttct ttttacggga aaagatgcgc gacggaaccc cgaaacggcc ggaatcagcg
     8701 attcgctgtt tttcggggcc ggcgacctca actgttttct gcgatccacc gaagcacaag
     8761 gtccggcctc ttccacgatt tcattttctg ctccatgcga aatgcttcgg atctcgtccc
     8821 gtattccctg ca
//
LOCUS       DASTSF010000137         3239 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_141084,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000137 DASTSF010000000
VERSION     DASTSF010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3239
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141084"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..91)
                     /gene="sufC"
                     /locus_tag="VFU15_04010"
     CDS             complement(<1..91)
                     /gene="sufC"
                     /locus_tag="VFU15_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014776200.1"
                     /note="part of SUF system involved in inserting
                     iron-sulfur clusters into proteins; in Escherichia coli
                     this protein forms a complex with SufBD; the SufBCD
                     complex stimulates the cysteine desulfurase SufS in
                     conjunction with SufE; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEU4716967.1"
                     /translation="MISIKNLHASVEGKEILRGVNLEVKDGEVH"
     gene            complement(322..1782)
                     /gene="sufB"
                     /locus_tag="VFU15_04015"
     CDS             complement(322..1782)
                     /gene="sufB"
                     /locus_tag="VFU15_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004583905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEU4716968.1"
                     /translation="MSDKNQILEEHISSDYKYGFVSDIDTEFAPKGLNEEIVRFISKK
                     KEEPEWLLEFRLKAFRHWLTMEEPKHWAHLSYPTIDFQDISYYAAPKKKTVVGSLEEV
                     DPELLKTFEKLGISLEEQKRLVGVESKIAVDAVIDSVSVKTTFRETLAEKGIIFCSFS
                     EAVKDYPDLVKKYMGSVVPYTDNFYAALNSAVFSDGSFCYIPKGVRCPMELSTSFRIN
                     SAGTGQFERTLIVADEGAYVSYLEGCTAPQRDENQLHAAVVEIIAEKDGEVKYSTVQN
                     WYPGDKNGKGGIYNFVTKRGICKGDNSRISWTQVETGSAITWKYPSVILKGNNSVGEF
                     YSVAVTNNYQQADTGTKMTHIGKNTRSTIISKGISAGKSNNSYRGLVRISKGAKNARN
                     FSQCDSLLMGDKCGAHTFPYIEIHDKSAVVEHEATTSKIGEDQIFYCNQRGIDTEKAI
                     GLIVNGYCREVLNQLPMEFAVEAQKLLSVSLEGSVG"
     gene            complement(1840..2166)
                     /locus_tag="VFU15_04020"
     CDS             complement(1840..2166)
                     /locus_tag="VFU15_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004920516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HEU4716969.1"
                     /translation="MITITESAKEHAISLIRAENRPGDTFIRVGVDSGGCSGLSYKLE
                     FDNQVLPTDQVFEDKGIRIVVDRKSFLYLVGTELDYTGGLNGKGFVFKNPNASRTCGC
                     GESFSV"
     gene            complement(2396..>3239)
                     /locus_tag="VFU15_04025"
     CDS             complement(2396..>3239)
                     /locus_tag="VFU15_04025"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEU4716970.1"
                     /translation="EFAVKVIKEGADDYILKNNMMRLPSAIDNAIKKKKAEREREQTL
                     AQLLLTNNELNTFVYKATHDLRGPLTSIMGLTNLAQREEDDSRLRAYVKMIGETTEKL
                     DEVLLSLIETMAIKNAQLTKEEINLPRLIPDILKNLRFVQGFDRVRFNLNISMTSAFI
                     SDKRILNSVLQNLIENSIRYQDHSKAISEVNISIADHKDGAMFEVADNGIGISENIQE
                     KIFDMFYRGHTGSKGSGLGLYILKNGVQRLGGKFELKSKPGFGTSFCIFLPSLQREDA
                     TVLP"
BASE COUNT          680 a          875 c          827 g          857 t
ORIGIN      
        1 cgtgcacttc gccgtctttc acttcaaggt tcacaccgcg caggatttct tttccttcaa
       61 cggaggcgtg gagatttttt atggagatca tttaattgcg ttgcagttta tggttggtag
      121 gttttaaagt cggaaggtct gcgaagttgt gttgttttct gtgaaataaa tctcttcagg
      181 ttcatgaaag gagctgccgc cggctttgtg acgaagatat acggtagatg ctgtcagcaa
      241 gagcacatga acgctgccat gccagaatat cctcaaatcg ctttatcgta ctcataaccg
      301 gaaacgagaa actttaaact tttacccgac actcccctcc agcgaaacag aaagtaattt
      361 ctgcgcttcc accgcgaact ccatcggcag ctggttcaat acttcccggc aatagccgtt
      421 cacgataagc ccgatcgctt tttccgtatc gatgccgcgc tggttgcagt agaagatctg
      481 gtcttcgccg atcttcgatg tagtggcttc atgctcgacg accgcgctct tgtcgtggat
      541 ctcgatgtag ggaaatgtat gcgcgccgca cttgtcgccc atgagcagcg aatcgcactg
      601 tgaaaagttg cgcgcgttct ttgcgccctt cgagatgcgc acgagcccgc ggtagctgtt
      661 gttgcttttc ccggcggaga tccctttcga gatgatcgtg ctccgcgtgt tcttcccgat
      721 gtgcgtcatc ttcgttcccg tgtccgcctg ctggtagttg ttcgtcacgg ccacggaata
      781 aaattcaccc acggaattat tccctttcag gatcaccgaa ggatatttcc acgtaatggc
      841 ggaaccggtt tccacctgcg tccacgagat gcgtgaatta tcgcctttgc agatgccgcg
      901 cttggtgacg aaattgtaga tgccgccttt cccgttcttg tcgccgggat accagttctg
      961 cacagtggaa tattttactt cgccgtcttt ttccgcgatg atctccacca cggccgcgtg
     1021 cagctggttc tcatcgcgct gcggggccgt gcacccttcg aggtagctga cgtacgcgcc
     1081 ttcgtcggcg acgatgagcg tgcgttcgaa ctggcccgtt ccggcggaat tgatgcggaa
     1141 agacgtgctc agctccatcg ggcagcgcac gcctttcggg atgtagcaga acgacccgtc
     1201 gctgaaaaca gccgagttga gcgccgcgta aaaattatcc gtgtagggaa cgaccgaacc
     1261 catgtatttt ttcacgaggt cgggatagtc cttcaccgct tcgctgaacg agcagaaaat
     1321 aattcctttt tccgccagcg tttcccggaa cgttgttttc acggaaacgg aatcgatcac
     1381 cgcgtcaacg gcgatcttcg attccacacc gacgaggcgt ttctgctcct cgagcgaaat
     1441 gccgagcttc tcgaatgttt tcagcagctc cggatccact tcttccaggc tgccgaccac
     1501 cgtttttttc ttcggtgcgg cataatagct gatgtcctgg aaatcgatcg tgggatagga
     1561 gaggtgcgcc cagtgcttcg gctcttccat cgtcagccag tggcggaacg ccttcaggcg
     1621 gaattcgagc agccattccg gctcttcttt ctttttcgaa atgaaacgga cgatctcctc
     1681 gttcagcccc ttcggcgcaa attcagtatc gatgtcggaa acaaaaccgt atttgtaatc
     1741 cgatgaaatg tgctcctcga gtatttgatt tttgtcgctc ataaatcatt caatgtttaa
     1801 tgttcaacgt cactgttcac cggcattgaa cattgaatcc taaacggaaa aactttcccc
     1861 gcagccacac gtgcgcgacg cattcgggtt tttaaacacg aatccttttc cgttgagccc
     1921 gcccgtataa tccagctccg ttcccacgag gtaaagaaaa cttttgcgat cgaccacgat
     1981 gcggatcccc ttgtcctcga aaacctggtc ggtaggcaat acctggttgt cgaattcgag
     2041 tttgtacgaa agacccgagc atccgccgct gtcaacaccc acacgaatga acgtatcccc
     2101 cggccggttt tcggccctga tcagggagat cgcatgctct tttgcactct ctgttatggt
     2161 aatcattgtt tttcagtgtt ttatggacaa gcccttattt agactaaatc caaataccgt
     2221 acaaaggtac ctaaaagaag gtatttgcaa aaagattttc aacagtaaat aagggatgaa
     2281 ttccgggagg agcggccgct gcgcagcatt gaattccctt tgttcccgct gccccgccat
     2341 gcacggcatt tgcgggcatc ggggctatgg aatttccgca ggtgttccaa aaaaatcagg
     2401 gcagcacggt agcgtcttcc cgctgaagcg aaggcaggaa aatgcagaac gaagtcccga
     2461 aaccgggttt gcttttcagc tcgaactttc cgccgaggcg ctgcacgccg ttcttgagaa
     2521 tgtaaagccc gaggccggaa cccttggaac ccgtgtgacc gcggtagaac atgtcgaaga
     2581 tcttttcctg gatgttctcg ctgatgccga tgccgttgtc ggccacttcg aacatcgcac
     2641 cgtccttgtg atcggcgatg gaaatattca cttcggagat cgccttcgaa tgatcctggt
     2701 atcggatgct gttctcgatc aggttctgga gaacggaatt caggatgcgc ttatccgaaa
     2761 tgaacgcgct ggtcatcgat atgttcaggt tgaaacgcac acggtcgaag ccctgtacga
     2821 aacgcaggtt cttgagaatg tcgggaatga gcctggggag gttgatctct tccttcgtga
     2881 gctgcgcgtt cttgatcgcc atcgtttcaa tgagcgaaag cagcacctcg tccagcttct
     2941 ccgtcgtctc cccgatcatc ttcacgtacg ctctcagccg tgaatcatct tcttcccgct
     3001 gtgcgaggtt ggtgagcccc atgatggacg taagcgggcc gcgcaggtcg tgcgtggctt
     3061 tgtagacgaa cgtgttcagc tcgttgttgg taagcagcag ctgcgccagt gtctgctcgc
     3121 gttcccgctc ggctttcttt ttcttgatcg cgttgtcgat ggccgagggc aggcgcatca
     3181 tgttattttt caggatgtag tcgtccgctc cttccttgat cactttcacg gcgaactct
//
LOCUS       DASTSF010000138        12510 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1103444,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000138 DASTSF010000000
VERSION     DASTSF010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 12510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12510
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1103444"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            22..1290
                     /locus_tag="VFU15_04030"
     CDS             22..1290
                     /locus_tag="VFU15_04030"
                     /inference="COORDINATES: protein motif:HMM:NF045576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BT_3928 family protein"
                     /protein_id="HEU4716971.1"
                     /translation="MTHITRIAVGGLFIFSGFIKANDPSGFSYKLEEYFEVFGQGFSC
                     DMKLPQKSMSGIPCEKDAGVSATTEAPKKPSVSVLKPLWDWFGEHSLFLAIFICAFEM
                     GVGLFLLLGIKVRWTLFLLLAMIVFFAFLTFYSACCNKVTSCGCFGDAIKLTPWESFW
                     KDLILLILIAVLFVGEKNISPLFKNSILIAGIAGIGLFASFAFPVYTWRHLPVIDFRP
                     YAIGTNLWTASHSKDPKTTGCVTDSTYSVYYYQNQRTGAMMHFHSLDSIPEDTIWQFF
                     CRDDRKVRTGNCGATVMDFILSDADGNDLTDSLLQMKGIQFFLVMYDIDKTETSDSAV
                     VNRFYSECAKNNVVFNGLSNGSGDQVDQFRKKTGAKFPMLTVDGTALKTVIRSNPGLV
                     MLKDGTVTAMWHINDFPAYGDVMAKYGGAK"
     gene            1294..2376
                     /locus_tag="VFU15_04035"
     CDS             1294..2376
                     /locus_tag="VFU15_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014682629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU4716972.1"
                     /translation="MGKFIIKRILYGFLVLFGVITTVFFLFNVLPGDPARMRMGQHSD
                     EKSLQIIRQDLNLDLPLYKQYLLFLNDLSFISVHDVKDSTSRYFLDSRKYGEPIALFS
                     AGGTKKIFLKAPYLRRSYMSQRPVWEIIKNTLPETVVLALTSIIFASIVGIGFGVLSA
                     LYKNSFWDRLVAVVSTLGMAGPSFFFALIISWLFGYLWKDYTGLNNIGTLFPLNVYTG
                     QEELVLKNLILPAFTLGIRPLALVVQLTRNSMLDVLSMDYIRTARAKGLGKLKVVTKH
                     ALKNALNPVITAISGWFAGLMAGAAFVEAVFDYKGIGNAVVEALFYYDLPVVMGSTLI
                     FSTCFVITNILVDVMYAFLDPRVRLQ"
     gene            2416..3192
                     /gene="tpiA"
                     /locus_tag="VFU15_04040"
     CDS             2416..3192
                     /gene="tpiA"
                     /locus_tag="VFU15_04040"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008241407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEU4716973.1"
                     /translation="MRTKIIAGNWKMNKTYAEALTLAAGVRDAVKGASHPGLLTLIAP
                     PFPFLAETAKLAEDIPGLHIAAQNCHQENAGAYTGEVSASMIASCGATHVIIGHSERR
                     NYFGENDATLLKKTMLTLEHSLVPVFCIGETLAERESKKHFETIAAQLENGLFPCGKE
                     AMKKIVIAYEPVWAIGTGVNATPAQAQEMHAFIRACVEKNSGKEIAAAVPVLYGGSCN
                     AGNAASLFACADVDGGLIGGASLRADEFASIRKSMLATLA"
     gene            3215..3988
                     /locus_tag="VFU15_04045"
     CDS             3215..3988
                     /locus_tag="VFU15_04045"
                     /inference="COORDINATES: protein motif:HMM:NF019284.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716974.1"
                     /translation="MKKAILLLLVFPALYAHAQSDVNQVCYHDTTLVDMIPGKKDTTY
                     AVKPQFRKGDWNIYYDFQFTKMKAECHYDKDGAKTGTWKSWFRDGEASEIWNYSGVLN
                     ANLPTGKSWYEKPYTLRSERIERNDSVIETDYFPDGKKQAVKCWTKDGMIVRDAQWCE
                     NGQLIVDFNPSSPTPLPVKKYYCNGKVKAEYSWFIFGYSGPYTEYDENGKVSVKGQFQ
                     DVPPGQKIFMPRKTGEWTYYDANGNVTKTENWSNGKLQK"
     gene            complement(3989..5461)
                     /gene="dacB"
                     /locus_tag="VFU15_04050"
     CDS             complement(3989..5461)
                     /gene="dacB"
                     /locus_tag="VFU15_04050"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HEU4716975.1"
                     /translation="MNALLRKIPLLLFLSVLFLQGTPPYDLQKEVDRLNGDTVMKHGE
                     WGLFALAQDSGNVIAENNADLSLMPASTMKILTTGAALGLLGPDFQYETTIGYDGTFD
                     KTSGTINGNLYIQGAGDPTLESKYFRRSDSATAFSSIAERLVSSGVKIITGDIIGDAS
                     CFSDNALPDGWTWGDLGQYYGAGTSGLDYRDNSVTLHFNSQGKDTCTIDSVTPFPENV
                     RYRSFVTADGKKDEAFVYGTPFGNDFSVYGTIPAGKKNYEVDASDPDPALLCAHDVLH
                     QLQGAGITVNGKCTSSGRIFRDGKKMPSAKRTVLFSLLSPPLSEIVHMTNLHSDNVYA
                     EQLLRTLGYRKGKSGSTEDGIDVVLDFWRSKGLDTDGLHMTDGSGLSRSNLITTKQQA
                     SVLRIISQSPWYDVFYRSLPVAGKSGSLAGLCKGTFAENNMHAKSGYIDRARGYAGFV
                     KTRSGKTICFSLLANNYTCPASEMKKRLERILVALAEYGG"
     gene            complement(5442..5687)
                     /locus_tag="VFU15_04055"
     CDS             complement(5442..5687)
                     /locus_tag="VFU15_04055"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020598283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HEU4716976.1"
                     /translation="MWTEANHTLSRSFVFHDFREAFAFMALVAFSAEKMNHHPDWRNA
                     WNKVDIHLSTHDAGNKVTEKDRLLAAEIDKIYERFAA"
     gene            complement(5747..6796)
                     /gene="porQ"
                     /locus_tag="VFU15_04060"
     CDS             complement(5747..6796)
                     /gene="porQ"
                     /locus_tag="VFU15_04060"
                     /inference="COORDINATES: protein motif:HMM:NF033711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system protein PorQ"
                     /protein_id="HEU4716977.1"
                     /translation="MKKLLVLFLVFSGYASLPAQIGGSAAFPSLNLALSARAASLGGR
                     AIAIKDNDLNLATWNPSLLSPEMTSQFAFSYTNYLTDIQYGYAGFAWNFKNIGTFDLF
                     ATRIDYGTFRETDNTGADIGTFKAGEYGFNLGYSRAIDSNFTIGANLKFIQSNLYLWK
                     ASGIAADIAATYKIPHHSFTASFLMRNIGRMISSYTPGDREKLPFEADAGFSFKPVHM
                     PFRFSFSFQQLQQWDLTYSDPNNPPLTVDPLTGDSIKTSKFRTFGDKLMRHVVVGGEL
                     LIGKPVSIRFGYDYMRRKELAIDTRKGASGFTFGIGIHVYKFNLSYAFVPYHLAGGTS
                     TFTLTTTLSEFYSKK"
     gene            6977..7357
                     /locus_tag="VFU15_04065"
     CDS             6977..7357
                     /locus_tag="VFU15_04065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716978.1"
                     /translation="MDTKPISELHAEHTEWLNKLDFYNDELIMMRERIQNVAAKNTGK
                     DILAKIEHFQNQIIVQRNNIDEFRHAIKDHENYLEHRIDENPVASDHRKVHDHPKLRE
                     TMESFEKIFNGIRHELNEFLAKVL"
     gene            complement(7404..8171)
                     /locus_tag="VFU15_04070"
     CDS             complement(7404..8171)
                     /locus_tag="VFU15_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009195083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU4716979.1"
                     /translation="MIITFLGTGTSQGVPLIGCRCATCTSPDPRDKRLRTAVMVETQG
                     KTLVIDTGPDFRQQMLRENVEKLDAVLLTHEHKDHLAGLDEVRAFNFLQKKSMPVYAT
                     QRVQEAIRREFAYIFETPQYPGIPKIDLHTISAHPFEAEGVPVEGIEVMHHQLPVLGF
                     RIGGFVYITDANFISPAEKEKIRGCELLVLNALRHEKHISHFTLAEAIAMVRELKPRR
                     ALFTHISHQMGRHADIQQELPEGISIAFDGQKAELPE"
     gene            8449..8808
                     /gene="ssb"
                     /locus_tag="VFU15_04075"
     CDS             8449..8808
                     /gene="ssb"
                     /locus_tag="VFU15_04075"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEU4716980.1"
                     /translation="MKMQNKQNGVKMVVNLGSNPTVKTVTGNRRMARFTVACLDRNGN
                     DPHAKMKWLPVVSWGSVAEMAGRFLRKGKRVLITGHVVERVWMDKKGNARTKSEIVAT
                     NLVLLDSGRIGVMRRAS"
     gene            8983..9324
                     /locus_tag="VFU15_04080"
     CDS             8983..9324
                     /locus_tag="VFU15_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014033789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEU4716981.1"
                     /translation="MNIIRNNVQLVGNLGQNPDIKELENGKKMARFSIACQESFLDRS
                     GKKTENTQWFTVVAWDGLAGIAEKYMHKGKQVAISGRLINRSWTDKEGNKKSITEIIA
                     SDILLLGGSKK"
     gene            9437..10846
                     /locus_tag="VFU15_04085"
     CDS             9437..10846
                     /locus_tag="VFU15_04085"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015028626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU4716982.1"
                     /translation="MPETGIHFMGAAQTVTGSRHLFGYGRTKFLLDCGMFQDKISIKA
                     DRNLEFGFDPTSVDFVILSHAHIDHSGSLPRLVKLGFRGKIYSTAATRDLCEVMLADS
                     AHIQVSDTEFRNQRRKAKGKPPLEPAYTPEDVKETMTRFQTVGYEQLTEIVPGLKFRF
                     TDAGHILGSAAVSLELKQDDSIIHVTFTGDIGRFHDLILKEPQPFPQADYILCESTYG
                     DRLHEATEDAALLLMNAVRHTCIEKKGKLLIPAFSLGRTQEIVYTLDRMQSNGLLPRI
                     PVYVDSPLSVDATEIMRHHPECFNAEIKTFMEKDPDPFGFRGLKYIQDVNESKALNFS
                     DEPCIIISASGMLDAGRIKHHVRNNIGKKSTTILMAGYCAPNTLGADLLNGDKKVRIF
                     GEMHEVKAEVIALHSYSAHADYKEILRFLGCQDSKKVKTLFLVHGEPEVQQHFRTTLE
                     KEGFANVQIPALGDYAVLK"
     gene            complement(10908..11150)
                     /locus_tag="VFU15_04090"
     CDS             complement(10908..11150)
                     /locus_tag="VFU15_04090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716983.1"
                     /translation="MKKSKLIAIAIFLFTFLVPFRMLVFRDDVIDNMGDIFLLLAVVA
                     GFFIGFGIGTNEPFDRKTSTLTASAEEIRNGQQKAA"
     gene            11306..>12510
                     /locus_tag="VFU15_04095"
     CDS             11306..>12510
                     /locus_tag="VFU15_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009578080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEU4716984.1"
                     /translation="MKDLFDKIRENRGPLGKHAKESHGYFTFPKLEGDIGPRMMFRGK
                     ERLVWSLNNYLGLANHPEVRKTDAEAAAKYGFAAPMGARMMSGNSNFHEQLEEGLSAL
                     TKKEDTILCNFGYQAMVSAIDCLVDRHDVIVYDAESHACIIDGVRLHMGKRFVYPHND
                     IASLEKQLERATKMVAETNGAILVLTEGVFGMSGNQGKLKEIVALKKKFNFRLFVDDA
                     HGCGTMGPNGGGTGEEQGCQDGIDIYFGTFAKSFALIGGFISSTEDIVEYLRYNMRSQ
                     IFAKSLPLPLVIGALKRLELMNTHKEFRENLWKITNALQSGLKANGFNIGTTNSPVTP
                     VYLSGSIPEATNLILDLRENFNIFCSMVVYPVIPKGQIILRLIPTATHSIEDVNYTID
                     AFSKIKDKLT"
BASE COUNT         3076 a         3257 c         3284 g         2893 t
ORIGIN      
        1 gtattcggga aattcaaatg gctgacgcac atcacgcgca ttgcggtcgg cggacttttc
       61 atcttttccg gtttcatcaa agcgaacgac ccttccggtt tcagctacaa gctcgaagaa
      121 tatttcgaag tgttcgggca gggcttcagc tgcgacatga agcttccgca gaaatccatg
      181 agcggtattc cctgcgagaa agacgccggc gtatcagcca cgaccgaagc tccgaaaaaa
      241 ccttcggtaa gtgttctcaa acctttgtgg gactggttcg gcgagcattc gctgttcctt
      301 gccattttca tctgtgcatt tgagatgggt gtcggccttt tcctcctgct cgggataaaa
      361 gtgcggtgga cgctgttcct gctcctggcc atgatcgttt tctttgcgtt cctcacattt
      421 tacagcgcgt gctgcaataa agtgaccagc tgcggctgtt tcggcgacgc gatcaagctc
      481 acaccgtggg aatcgttctg gaaagacctg atccttctta ttctcattgc agttcttttt
      541 gtgggtgaaa agaacatttc accgctgttc aaaaacagca tccttatcgc gggcattgcc
      601 ggcatcggtc tttttgcttc attcgctttc ccggtgtata cgtggcgcca cctcccggtg
      661 atcgatttcc gcccgtacgc gataggaacg aacctgtgga cggcttcgca ttcgaaagat
      721 ccgaagacaa cgggatgtgt aacggattcg acgtattctg tttattatta ccagaaccag
      781 cggaccggcg ccatgatgca tttccattcg ctcgattcga ttcccgagga tacgatctgg
      841 caatttttct gccgtgacga tcgcaaagtg cgaacgggca attgtggcgc gaccgtgatg
      901 gattttattc tttccgatgc ggatggcaac gaccttaccg attcgctgct gcagatgaaa
      961 ggcatccagt ttttcctcgt catgtacgac atcgacaaaa cggaaacatc cgacagcgca
     1021 gtggtcaacc gcttttattc cgaatgtgcg aagaacaatg ttgttttcaa cgggctttcg
     1081 aacggcagcg gcgaccaggt ggatcaattc aggaagaaaa caggagctaa gttccccatg
     1141 ctgacggtag acggaacagc gctcaaaaca gtcatccgct ccaaccccgg gctggtaatg
     1201 ctgaaagacg gaaccgtcac tgccatgtgg cacatcaacg actttccggc ttacggcgat
     1261 gtcatggcga aatacggagg agcgaagtaa gcggtgggaa agttcatcat caaacggata
     1321 ctgtacgggt tcctcgtttt gttcggggtg atcacgaccg tgttcttcct gttcaacgtg
     1381 cttcccggcg atcctgcgcg catgcgcatg ggacagcatt ccgatgaaaa atcactgcag
     1441 atcatccggc aggatctcaa cctcgatctc ccgctttaca agcaatacct gctttttctc
     1501 aatgaccttt cgttcatctc cgtccatgac gtgaaggatt ccaccagccg ctatttcctc
     1561 gattcgcgca agtacggtga accgatcgct cttttcagcg cgggaggaac gaaaaaaata
     1621 ttcctgaaag ctccgtatct ccggcgctcg tacatgtcgc agcggccggt gtgggagatc
     1681 atcaagaaca cgctcccgga aacggtggtg ctcgcgttaa cctcgatcat cttcgcctcc
     1741 atcgtcggca tcggtttcgg cgttctttcg gcgttgtata aaaattcgtt ctgggaccgg
     1801 cttgtcgccg tcgtttcaac gctcgggatg gcaggaccat ctttcttttt tgcgcttatc
     1861 atttcctggc tgttcggtta tttatggaaa gattacacgg ggctcaataa catcggtacg
     1921 cttttcccgc tgaatgttta cacagggcag gaagagctcg tgctcaaaaa ccttatcctt
     1981 cctgcgttca cgctggggat acgtccgctg gccctcgtcg ttcagctgac gcgcaattcc
     2041 atgctcgatg ttctttcgat ggattatatc cgcaccgcgc gtgcaaaagg tctcggaaaa
     2101 ctaaaagtgg tgacgaagca cgcgctgaag aacgcgctga acccggtgat caccgcgatt
     2161 tccggatggt tcgcgggcct catggccgga gccgctttcg tggaagcggt tttcgattac
     2221 aaagggatcg gcaacgccgt cgtggaagct cttttctatt atgatcttcc cgtcgtcatg
     2281 ggaagcacgc tcattttctc cacctgcttc gtgatcacga atattcttgt cgacgtcatg
     2341 tatgctttcc tcgacccgag ggtaaggctg cagtaggctt tctctccacc tttttttgcc
     2401 gtagatttcc tttttatgag aacaaagatc atcgccggta actggaaaat gaacaaaacg
     2461 tacgcggaag cgctgacgct tgcggccggt gtgcgtgatg cagtgaaagg ggcttctcat
     2521 cccggcctgc ttacgctgat cgctcctcct tttcctttcc tcgccgaaac agcgaagctg
     2581 gcggaagata ttcccggact ccacattgcc gcgcaaaact gccaccagga aaatgcaggt
     2641 gcgtatacag gagaggtttc agcctccatg atcgcttcct gcggagcgac acacgtgatc
     2701 atcgggcaca gtgaacggag aaattatttc ggcgaaaacg acgcaacgct gctgaaaaaa
     2761 acaatgctca cgctggagca ttcgcttgtc ccggttttct gcataggcga aacacttgcg
     2821 gaacgggaaa gcaaaaaaca ttttgaaacg atcgcggcac agctggaaaa cgggcttttt
     2881 ccctgcggaa aagaagcgat gaaaaagatc gtcattgctt atgaaccggt atgggcgatc
     2941 ggtaccggcg ttaacgccac gcctgcccag gcgcaggaaa tgcacgcatt catccgcgcc
     3001 tgcgtggaaa aaaattccgg gaaggagatc gcggcagctg ttcctgttct ttacggcggg
     3061 agctgcaatg ccgggaatgc cgcttctctc ttcgcgtgcg cagacgtcga cggcggactt
     3121 atcggcggcg cttcgctcag ggccgacgaa tttgcgtcca tccggaaatc catgctcgcc
     3181 actctggcat gagatgtgca gttccagttt aacaatgaaa aaagcaatcc tcctccttct
     3241 tgttttcccg gcgctttacg cccatgcaca gagcgatgtg aaccaggtct gttaccacga
     3301 tacaacgctc gtcgatatga tcccgggcaa aaaggatacg acatacgctg tcaaaccgca
     3361 attcaggaaa ggcgactgga atatctatta cgatttccag ttcacgaaaa tgaaagcgga
     3421 atgccattat gataaagacg gggcgaaaac cgggacgtgg aaaagctggt tccgcgacgg
     3481 ggaagcgagc gagatatgga attattccgg cgtgctgaat gcgaacctgc ctaccgggaa
     3541 atcctggtat gaaaaacctt acacgcttcg gtccgagcgc atcgagcgca acgattccgt
     3601 cattgaaacg gattactttc ccgacggaaa aaagcaggcg gtcaagtgct ggaccaaaga
     3661 cggcatgatc gttcgtgatg cacagtggtg tgaaaacggc cagctcatcg tcgatttcaa
     3721 cccgagctcc cctactccgc tgccggtgaa aaaatattat tgcaacggga aagtaaaagc
     3781 ggaatacagc tggttcattt tcggttattc cgggccttat accgagtacg acgagaacgg
     3841 aaaagtttcc gtgaaaggac agttccagga tgttccgccg ggacagaaaa tattcatgcc
     3901 gcgcaagacc ggtgaatgga cctattacga cgcgaacgga aacgtgacga agaccgagaa
     3961 ctggagcaac gggaagctgc agaaataatc acccgccgta ttcggcgagc gccacgagaa
     4021 ttctttccag cctttttttc atttcagaag cagggcaggt ataattgttc gccagcagcg
     4081 agaagcagat cgttttaccg ctgcgtgttt tcacgaatcc cgcgtatcct cttgcccggt
     4141 cgatgtaacc gctttttgca tgcatgttat tttcagcgaa tgttcctttg cagagcccgg
     4201 caagcgatcc gctttttccc gccacgggaa gagaacggta gaaaacatca taccacggcg
     4261 actgtgaaat gatgcgcagc acactggcct gttgtttcgt ggtgatgaga ttggaacgcg
     4321 agagcccgct tccatccgtc atgtgcaggc cgtccgtgtc caatcctttc gaacgccaga
     4381 aatccagcac gacgtcgatc ccgtcttcgg tcgaaccgct tttgcctttc cggtaaccga
     4441 gcgtccgcaa cagttgctcc gcgtacacat tatcgctgtg caggttcgtc atgtgaacga
     4501 tctccgaaag cggtggcgaa agcaaagaaa aaagaaccgt gcgttttgcc gaaggcattt
     4561 tctttccatc gcggaatatc cttccggatg aagtgcattt tccgttcacc gtaatgccgg
     4621 cgccctgcag ttgatgcagc acatcatgcg cgcagagcaa tgcgggatcg ggatcggagg
     4681 catcgacttc gtaatttttt ttccccgcgg gaatagttcc gtaaacggaa aaatcatttc
     4741 cgaaaggtgt gccgtaaaca aatgcttcgt ctttcttccc gtccgcggtt acaaaagaac
     4801 ggtaacgcac gttctccggg aaaggcgtca cgctgtcgat ggtgcaggtg tcttttccct
     4861 gtgaattgaa atgcagcgtc actgaattat cgcggtaatc aagcccgctg gtgccggcgc
     4921 cgtaatactg gccgaggtcg ccccaggtcc acccgtcggg cagcgcattg tcgctgaagc
     4981 aggacgcatc accgataata tccccggtta ttattttcac gcctgaggaa accaggcgct
     5041 cagcaatgct gctgaaggcg gtagcgctgt cgcttctccg gaaatatttc gattcgagtg
     5101 tcgggtcacc ggctccctgg atgtacaggt ttccattgat agttccgctg gtcttgtcga
     5161 acgtcccgtc gtatccgatc gtcgtttcgt attgaaaatc cgggccgaga agtccgagcg
     5221 cagcgccggt ggtgaggatc ttcatcgtgc tcgcgggcat gaggctgaga tcggcgttgt
     5281 tttccgcgat cacgttcccg ctgtcctgcg ccagggcgaa aagcccccac tccccgtgct
     5341 tcataacagt gtcgccgttc aggcgatcaa cttctttctg cagatcataa ggaggcgttc
     5401 cctgcagaaa aagcacggac aaaaaaagca gcaacgggat cttacgcagc aaagcgttca
     5461 tagatcttat cgatttccgc tgcgagcaaa cgatcctttt cagtgacctt gttcccggca
     5521 tcgtgcgttg aaagatggat atccaccttg ttccacgcat tgcgccagtc gggatgatgg
     5581 ttcatcttct ccgccgaaaa agcgacgagg gccatgaagg cgaaggcttc gcggaaatca
     5641 tggaagacga aactgcggga aagagtatga ttggcttcgg tccacataat gcatgttgtt
     5701 tcttcaaaag taggagaaac agcagacaag ccggaatgca aatgcgctac tttttcgaat
     5761 agaactccga aagcgtcgtg gtgagtgtga atgtgcttgt tcccccggca aggtgatacg
     5821 gaacgaacgc gtagctgagg ttgaacttgt aaacgtggat gccgatgccg aacgtgaatc
     5881 cgctggcgcc tttgcgcgtg tcgatggcaa gctctttccg gcgcatgtaa tcatatccga
     5941 aacggatgga aaccggtttg ccgatgagca attctcctcc caccaccacg tgacgcatca
     6001 gcttgtcgcc gaacgtgcgg aacttgcttg tcttaatgga atcgccggtg agcggatcta
     6061 cggtaagcgg aggattgttc ggatcgctgt aggtaagatc ccattgctgc aactgctgga
     6121 acgaaaatga aaaacggaac ggcatgtgaa cgggcttgaa tgaaaaaccc gcgtccgctt
     6181 caaaaggaag cttctcacgg tcgcccggcg tatacgatga gatcatccgg ccgatgttcc
     6241 tcatcaggaa agaggccgtg aagctgtggt gcgggatctt gtacgtggcg gcaatatcgg
     6301 cggcgatgcc gcttgctttc cagaggtaaa gattgctctg gatgaatttc aggttggcgc
     6361 cgatcgtgaa attgctgtcg atggcgcgcg aatacccgag attgaaacca tattcgccgg
     6421 ctttgaatgt cccgatatcg gcgcccgtgt tgtccgtttc ccggaaagtg ccatagtcga
     6481 tgcgcgtggc gaaaagatcg aacgtgccga tatttttaaa gttccaggcg aaaccggcat
     6541 agccgtattg aatgtcggta aggtaattgg tgtagctgaa cgcgaactgg ctcgtcattt
     6601 ccggtgaaag cagtgacggg ttccacgtcg ccaggttcag gtcattgtcc ttgatagcga
     6661 tcgctctgcc accaagtgaa gctgcgcgcg cagaaagcgc caggttgagt gaaggaaaag
     6721 cagcgctgcc gccgatctgt gcaggaagcg aagcatatcc ggaaaatacg aggaataaaa
     6781 cgagcagttt cttcatccag gtgaaagcca aagataacaa caaggccatt aacggggaaa
     6841 tcacgggctt attgcatttc cggcgaacaa aaaggccgcg taaaatgagc aaaatcaccc
     6901 ggtatgtgcg gcctgtgtca taatttgcag aaagaacggc ctgcatcttt gtacaactaa
     6961 aaaaacaccc gcgtttatgg acacaaaacc tatctccgag ctccacgctg agcacaccga
     7021 atggctgaac aagctggatt tttataacga cgagctgatc atgatgcgtg aacgcatcca
     7081 gaacgtggca gcaaaaaata ccggcaagga tatcctggcg aagatcgaac acttccagaa
     7141 ccagatcatt gttcagcgga acaacattga cgaattccgt catgcgatca aggatcatga
     7201 aaattatctc gagcaccgta tcgacgagaa cccggttgct tccgatcacc ggaaggttca
     7261 cgatcatccg aagctgaggg aaacgatgga aagctttgag aaaattttca acgggatccg
     7321 tcacgagctg aatgaatttc tcgcgaaagt tctttaagaa gtaaaagagg aaaatgcaaa
     7381 gccggcccgg atggccggct tttttattcg ggcagttctg ccttttgtcc gtcaaacgcg
     7441 atcgaaattc cttcgggaag ctcttgctgt atgtcagcgt gcctgcccat ctggtggctg
     7501 atgtgcgtaa ataaggcgcg ccggggtttc agctccctga ccatggcgat cgcttcggcg
     7561 agtgtgaaat gcgaaatatg tttttcgtgc cggagcgcat tcagcaccag cagctcacag
     7621 cccctgatct tttctttttc agcgggagaa ataaaatttg catccgtgat gtacacaaac
     7681 ccgccgatcc ggaaaccgag aacgggaagc tggtgatgca tcacctcgat cccttccacc
     7741 gggacgcctt ccgcttcgaa cgggtgcgcc gaaatggtgt gcagatcgat cttcggtata
     7801 cccggatatt gcggcgtttc aaaaatgtag gcgaattcgc gccgtatcgc ttcctgcacg
     7861 cgttgcgttg catacacggg catgcttttc ttctgcagga aattaaacgc ccgcacttca
     7921 tcgagtccgg ccaggtgatc cttgtgctca tgcgtgagca ggacggcatc gagcttttcc
     7981 acgttttccc gcagcatctg ctgccggaaa tccggtcccg tgtcgatcac cagcgttttt
     8041 ccctgcgtct ccaccataac agcggtccgc aggcgcttgt cccgcgggtc aggtgatgtg
     8101 cacgtggcgc accggcagcc gataagcggc acgccctgcg aggttccggt tccgagaaag
     8161 gtgatgatca tgatcggaag caaatttacc attagttcat cgcagaaatt tgaattttga
     8221 tttaacctga ttcccaggta catattcgtt tttaaacgac taaaagcgat tatttccgga
     8281 aataaacggt tgttaaccgg ctgacggaat gaggccgcca cacctttgca ccgtcgttct
     8341 ttcacaacaa cagaaaatca ggatcatatg cagaaggagc attactcccg acgcaaatat
     8401 gcaagcgcaa gtacaattcg atcagtttaa cctttaaaac ttttatttat gaaaatgcag
     8461 aacaaacaga acggtgtgaa gatggttgtg aacctcgggt ccaatcccac agtaaaaaca
     8521 gtaacaggca accggcgcat ggcgcgtttt acggtggcgt gccttgaccg caacgggaac
     8581 gatccccatg caaagatgaa atggcttccc gttgtttcat ggggaagcgt ggcagaaatg
     8641 gccgggcgct tcctgcgcaa agggaaacgt gtgctcatca ccggccatgt agttgaacgt
     8701 gtgtggatgg ataaaaaagg aaatgcccgc acgaagagcg agatcgtcgc caccaacctc
     8761 gtgctgctcg acagcggcag gatcggcgta atgcgccgcg cctcgtaaat cgcggtgacg
     8821 aggtccgggt attccgggca gcggcaggct tgcctgggag aattcttccg ggtgccggtt
     8881 cgcgccgcct tccctctcct tcgcatttta tttcttgatc attttaatta ttcaatccgt
     8941 aaccaggtat tacttatcac taatcattaa tcacttatta tcatgaacat catccgcaac
     9001 aacgtgcagc tcgtaggcaa cctcggtcag aatccggaca tcaaagaact cgaaaacggg
     9061 aaaaaaatgg cgcgtttctc aattgcctgc caggaatctt tcctcgaccg ttccggtaaa
     9121 aaaacggaga acacgcagtg gtttactgtc gtggcgtggg acggtcttgc cggcatcgca
     9181 gaaaaataca tgcacaaagg gaagcaggtg gcgatttccg gccgccttat caaccgctca
     9241 tggacagaca aggaaggcaa caaaaaatcc atcacggaga tcatcgcgag cgacatactg
     9301 ctgctcggcg gatcaaaaaa ataaattccg gggccatgcg ctttggtttt tggttgcggg
     9361 gccttcgggc cccttttttc ttttatcatt tttcgaccgc gataaaactt tagcttcggc
     9421 aattaaatac agcattatgc ctgaaacggg aatacatttc atgggggcag cgcagacagt
     9481 caccggcagc cgtcacctct tcggatacgg aaggacaaaa ttcctgctcg actgcgggat
     9541 gttccaggat aaaatttcca tcaaggcaga ccggaatctc gaattcggtt ttgatccgac
     9601 ctcggtggat tttgtcatcc tcagtcatgc gcacatcgat cacagcggca gtcttccgcg
     9661 gctggtgaag ctcggctttc gcggaaaaat atattccact gcggccactc gcgacctgtg
     9721 cgaagtgatg ctggccgaca gcgcgcatat ccaggtaagc gataccgaat tcaggaacca
     9781 aagaagaaag gcgaaaggga aaccgccgct ggaaccggct tacaccccgg aggacgtgaa
     9841 agaaaccatg acgcgtttcc aaaccgttgg gtacgaacag ctgacggaga tcgtcccggg
     9901 tctgaaattc aggttcacgg acgccgggca catactcgga agcgcggcag taagtctcga
     9961 gctgaaacag gatgattcca tcatccacgt tacgttcacc ggcgacatcg ggcgctttca
    10021 cgatcttatt ttgaaagaac cgcagccttt tccgcaggcg gattacattc tctgcgaatc
    10081 gacctacggc gaccgcctgc atgaagcgac ggaagacgca gcgctccttt tgatgaacgc
    10141 ggtgcgtcat acctgcatcg aaaaaaaggg caagctgctt attcccgcgt tttccctggg
    10201 ccgcacacag gagatcgtgt atacgctcga ccgtatgcag tctaacggat tacttccgag
    10261 gataccggtt tacgtcgaca gcccgctttc cgttgacgca acggagatca tgcgtcatca
    10321 cccggaatgt ttcaatgcgg aaataaaaac gttcatggaa aaagacccgg acccgttcgg
    10381 tttccgcgga ctgaaataca ttcaagacgt taatgaatcg aaggcgctga atttttcaga
    10441 tgaaccttgc attatcatat ccgcatccgg catgctcgat gcgggccgta tcaagcacca
    10501 cgtgcgcaac aatatcggga agaaatccac cacgatcctt atggccggtt attgtgcgcc
    10561 caacacgctc ggcgccgacc tgctgaacgg cgataagaaa gtcaggattt tcggtgaaat
    10621 gcacgaggtg aaagccgaag tgatcgcgct ccattcctac agcgcgcacg ccgactataa
    10681 agagatcctg cggtttctcg gttgccagga cagtaaaaaa gtgaaaacgc tgttcctcgt
    10741 ccacggagag ccggaggtgc agcagcattt ccgtacaacg ctggaaaaag aaggatttgc
    10801 aaacgtgcag atacccgcac tcggtgatta tgccgtgctg aaataaaaaa gtacccgctc
    10861 gtgccggacg caggcgggta cccaacacct cacagggagg ttgtttatca ggcagctttc
    10921 tgctgcccgt ttcggatttc ttctgcggaa gctgtaagag tggatgtttt ccggtcgaaa
    10981 ggctcgtttg ttccgatccc gaacccgata aaaaaaccgg ccactacggc gagaagaaga
    11041 aatatatcac ccatgttatc aatgacgtca tccctgaata ccagcatacg gaaaggaacc
    11101 aggaatgtga acaggaaaat ggcgatcgcg ataagtttac tttttttcat gacgcaggaa
    11161 ttttgtttgt caaaaatatc agcgccaccg ggtcagttcg catgacggac agcaggcgcc
    11221 gggctgacct tcgtcagtcc gggtggttga tcaaacacgg ctttctttta cctttgtgtc
    11281 ttcaatccat aaccaatcct taactatgaa agaccttttc gacaagatcc gcgagaaccg
    11341 cggtccgctg ggcaagcacg ccaaggaatc acacggttat ttcacttttc caaagctgga
    11401 aggcgatatc ggtccacgga tgatgttccg cggaaaggaa aggctggtct ggagcctgaa
    11461 taattatctc ggcctggcca atcaccccga agtgcgcaaa acagatgcag aggcggcagc
    11521 gaaatacggt ttcgccgcac cgatgggcgc gcgcatgatg tcgggaaatt ccaattttca
    11581 tgagcagctc gaagaaggtc tttcggcatt gaccaaaaaa gaagatacga tcctctgcaa
    11641 cttcgggtac caggcgatgg tttctgccat tgactgcctt gtcgaccgcc acgatgtgat
    11701 cgtttacgac gcagaatcgc atgcgtgcat cattgacggc gttcgcctgc acatgggaaa
    11761 acgtttcgtt tacccgcata atgatatcgc ttcgcttgaa aagcagctgg agcgcgcaac
    11821 gaaaatggtg gcggaaacaa acggcgccat cctcgttctt accgaaggcg ttttcggaat
    11881 gagcgggaac cagggcaagc tgaaagagat cgtggcgctg aaaaagaaat tcaatttccg
    11941 ccttttcgtc gacgatgcgc acggatgcgg aacgatgggc cccaatggcg gcggtacggg
    12001 agaagagcag ggttgccagg acggcatcga tatttacttc ggcacgtttg cgaaatcgtt
    12061 cgctctcatc ggcggattta tttcttcaac agaggacatc gtcgaatacc tgcgctacaa
    12121 tatgcgttcg cagatcttcg caaaatcact cccgctcccg ctcgtgatcg gcgcgctcaa
    12181 gcgtctcgag ctgatgaaca cgcataagga attccgtgag aatctctgga agatcacgaa
    12241 tgcgctgcag tcgggtctca aagcgaacgg cttcaacatc ggcacgacga attccccggt
    12301 cacgccggtt tacctcagcg gttccattcc cgaagcgacg aacctcatcc tcgacctgcg
    12361 cgagaacttc aacatcttct gctccatggt cgtttatccc gtgatcccga aaggccagat
    12421 catcctccgc ctgattccca cggcaacgca ttcgatcgaa gatgtgaact acacgatcga
    12481 tgccttctcg aagatcaagg acaagctgac
//
LOCUS       DASTSF010000139         4300 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_140839,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000139 DASTSF010000000
VERSION     DASTSF010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4300
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_140839"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..337)
                     /locus_tag="VFU15_04100"
     CDS             complement(<1..337)
                     /locus_tag="VFU15_04100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4716985.1"
                     /translation="MPGANLPNTGKIELMIVGAQKAGTTSLKFYLGEHPRIQSHLHKE
                     FAYFTDISEYGDGMNAAWKKYFGKIDPEKHIVVKNAGIYANENGLKLLSRHNPDCRIA
                     IVLRNPVDRT"
     gene            384..968
                     /gene="rdgB"
                     /locus_tag="VFU15_04105"
     CDS             384..968
                     /gene="rdgB"
                     /locus_tag="VFU15_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014904964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HEU4716986.1"
                     /translation="MVLVFASGNEGKLREINSLLPVDFRAESLAAFTSETDIAEPYFT
                     FAENAMHKAKEAFRITGLPSFADDSGLEVRALGGKPGVFSARYAGEKKDPSANNEKLL
                     REMENVAGREARFRTVIAFYDGKNGKLFEGVVEGRIAHSPRGSNGFGYDPLFIPGNSD
                     RTFAEMELAEKNRNSHRAKAFAAFLGWLKTLPGQ"
     gene            1081..3795
                     /locus_tag="VFU15_04110"
     CDS             1081..3795
                     /locus_tag="VFU15_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017732551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEU4716987.1"
                     /translation="MKIRVGIVFGGMSREREISFAGGRTVYDNLDKDLFEPVPVFVDS
                     FGNFILLNWEFIYKGSIRDFYPPVDVVPHSPGDFQVYAESLEPSPAEIEKMIAALGKH
                     IQPRELKTLFDFAFLCLHGPFGEDGRIQGFFEYLGIPYSGSGILPSAIGIDKIVQKKL
                     MSDAGFSTPAFFSVSRGEWFGGQQEAVFKKAEKTVGFPLVVKSATQGSSIGISILQNA
                     SFGGFRDAVNKSFFTAEITVEEWNGRDADAKNNFIRVLTDIREGIGLPVVMNANGKQE
                     TIFHPDALAEKISKHFASASSPVFLEALDAESEILIEGFIEGKEFSCIVVENETGEGR
                     EPVALPPTEIRKGKELFDYRSKYLPGLSRKITPIELPDAQIDAIRRECERLFRTLGFD
                     VYARIDGFISKDGKIFLNDPNTTSGMMPSSFFFHQAAEIGLNPSQFLTYIIRTSLRAR
                     IRADKNGKRYDRFLAALDEAMHASRSRVEKKTRVAVILGGYSSERHISVESGRNVYEK
                     LASSSKYTPVPVFLSGNAESFRLFVIPVNILLKDNADDIREKIEHWKVHPVIRAVISE
                     CGSITGMYSDANALAAPRGITFKELAQEADAVFIALHGRPGEDGAVQQQLDAVGLPYN
                     GSGSDSSAITIDKYRTNEILKENGFLVAEHMLVSEEEWNRNKAEILSRIEEKIHWPFI
                     AKPVDDGCSSAVRKIKTKEELEAFAKLIFRKAEDLDAASASLLKIKPKEEFPLKRVFL
                     VEELISKKEAKHFLEVTGGMLTRYNAKGEVEYEVFEASEALSEGEVLSLEEKFLAGQG
                     QNITPARYSADPAERQQISAKVKATLGAAARLLNIQGYTRIDAFVRIYNPEKVEVIFV
                     EVNSLPGMTPATCIFHQAAISHYKPYDFIDKILDFGFARRDRKISQPA"
     gene            3803..>4300
                     /locus_tag="VFU15_04115"
     CDS             3803..>4300
                     /locus_tag="VFU15_04115"
                     /inference="COORDINATES: protein motif:HMM:NF015734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HEU4716988.1"
                     /translation="MKKFFSFFKSRAFFINLGIAVILLPLLFWIIFAWMASYTRHGDF
                     VVVPDFTNLKINQLNSFVDDKSVSYEIIDSIWDPKLQKGLVLRQMPAPGAKVKQGRKV
                     YLYVTATHAPEIAMPKLVDLSMRQAMAVCESYGLKAEFRSIDDPCDGCILKQEYKKKP
                     INPGDP"
BASE COUNT         1030 a         1083 c         1184 g         1003 t
ORIGIN      
        1 aggtgcggtc taccgggttg cgcagcacga tggcgatgcg gcagtcgggg ttgtgcctgc
       61 tgagcagctt cagcccgttc tcgttcgcat agatgccggc gtttttcacg acaatgtgtt
      121 tttccggatc gatcttcccg aaatattttt tccacgcagc gttcatcccg tcgccgtatt
      181 cggaaatatc cgtgaaatag gcgaattcct tatgaagatg cgactgtatg cggggatgtt
      241 ccccgagata aaacttgagc gacgttgttc ccgctttctg cgcacccacg atcatcagct
      301 cgatcttccc tgtgtttggt aaatttgccc cgggcataga tgaccaaatt agcaacttaa
      361 tttcaaagga aggttacaga attatggttt tggtttttgc atcagggaat gaaggaaagc
      421 tcagggagat caattcgctt ttgccagttg atttccgtgc agaaagtctt gccgctttca
      481 caagtgaaac ggatatcgcg gagccgtatt tcacgttcgc ggaaaatgcc atgcacaagg
      541 cgaaagaagc tttccgcatc accgggctgc cttcgtttgc cgatgacagc gggctcgagg
      601 tgcgggcgct cggtggaaaa ccgggtgttt tttccgcacg ttatgcgggt gaaaaaaaag
      661 atccgtccgc caacaatgaa aaactgctcc gtgaaatgga aaatgttgcc gggcgcgaag
      721 cgcgtttccg caccgttatc gctttttacg acgggaagaa cgggaagctc ttcgaaggag
      781 tggtcgaagg ccggatcgcg cattctccgc gcggaagcaa cggcttcggt tatgatccgc
      841 tgtttatccc tggaaattcg gaccgcacat ttgccgaaat ggagctcgcg gaaaagaaca
      901 ggaacagtca ccgcgcgaaa gcatttgccg ctttcctcgg ctggctgaaa acgttgcccg
      961 gtcagtgaca cggcagattt cccgcatttc tttacgccgg gaatgttttt aacaacacct
     1021 attgaaaacc cgcgttctgc ccgaccggca ggctacccca aaagcctatc tttgttacct
     1081 atgaaaatcc gcgttggaat tgtattcggc ggcatgtcga gagagcgtga gatctcgttt
     1141 gccggcggga gaacggtata cgataacctg gataaagacc tgttcgagcc tgtgcccgtt
     1201 tttgtggaca gcttcgggaa tttcatcctg ctgaactggg aattcattta caaaggaagc
     1261 atccgcgatt tttacccgcc cgtggatgtc gtgccgcatt cacccggcga tttccaggtg
     1321 tacgcggaat cgctggaacc gtccccggcg gaaattgaaa aaatgatcgc agcgctggga
     1381 aaacatattc agccccgcga actgaaaacc ctgttcgatt ttgcgttcct ctgcctgcac
     1441 ggacctttcg gcgaggacgg ccgcatccag ggattttttg aatacctcgg cattccttat
     1501 tcgggcagcg gcattcttcc ttctgccatc ggcattgata agatcgtgca gaaaaagctg
     1561 atgtcggatg ccggcttcag cacgcccgct tttttttccg taagccgggg cgaatggttc
     1621 ggcggacagc aggaagctgt tttcaaaaaa gcggaaaaga ccgtcgggtt tccgctcgtg
     1681 gtgaaatccg ccacgcaggg ttcttccatt ggtatttcca ttttgcagaa cgcatctttc
     1741 ggcggattcc gggacgcggt gaacaaaagc tttttcaccg cggagatcac cgtggaagaa
     1801 tggaacggca gggatgcgga tgcaaagaat aatttcatcc gtgtgctgac cgacatccgt
     1861 gaagggatcg gtttgccggt ggtgatgaac gcgaacggaa agcaggaaac gatctttcat
     1921 cccgacgcgc tcgcagaaaa aataagtaag cattttgctt cagcgtcatc gccggttttt
     1981 cttgaagcgc tcgatgcgga aagtgaaata ctcattgaag gttttataga gggaaaagaa
     2041 ttttcctgca tcgtcgtcga gaatgagaca ggtgagggaa gagaacctgt agcgttaccg
     2101 ccaacggaaa tacgtaaagg caaggagctg ttcgattacc gttccaagta tcttcccggc
     2161 ctttcacgca agatcacgcc cattgaattg ccggatgcgc agatcgacgc gatccgcagg
     2221 gaatgcgaac gtcttttccg tacgctgggt ttcgatgtct atgcaaggat tgacggcttc
     2281 atctccaaag acgggaaaat attcctcaac gaccccaaca cgacttcggg catgatgcct
     2341 tcttcgttct tctttcacca ggcggcggag atcgggctca atccttcgca gttccttact
     2401 tacatcattc gcacttcgct ccgcgcgcgc atcagggcgg acaagaacgg gaaacgttac
     2461 gaccgttttc ttgctgcgct ggatgaagcc atgcatgcgt cgcgctcgcg cgtggaaaag
     2521 aaaacgcgtg tcgccgttat tctcgggggc tattcttccg agcggcatat ttcggtggaa
     2581 agcgggcgca atgtgtatga aaagctggct tcctcttcga agtatacgcc ggtgccggtt
     2641 ttcctttcgg gaaacgcgga atcgttcagg ctttttgtta ttcccgtgaa catcctgctg
     2701 aaagataacg ccgacgacat tcgtgaaaag atcgagcact ggaaagttca tccggttatc
     2761 cgcgctgtta tttccgaatg cggttccatt acggggatgt attccgatgc aaatgcgctg
     2821 gcggctccgc gcgggatcac gttcaaagag ctcgcacagg aagccgatgc ggttttcatc
     2881 gcgttgcacg gacgtcccgg cgaggatggt gccgtgcagc agcagctcga cgcggtggga
     2941 cttccctata acgggtccgg cagcgacagc tcggcgatca cgatcgacaa gtaccgcacg
     3001 aatgagatac tgaaagagaa cgggtttctc gtggcggagc acatgctcgt ttccgaagag
     3061 gaatggaaca gaaacaaggc ggagatactc agccgcatcg aagaaaaaat tcactggccg
     3121 ttcatcgcga agccggtcga cgacggatgc agctccgccg ttcgaaaaat aaaaacgaag
     3181 gaagagctgg aggcgtttgc aaagctgatc ttccgcaaag cggaagacct cgatgcagcg
     3241 tccgcatcgt tgctgaaaat aaaaccgaag gaagaattcc cgctcaagag agttttcctc
     3301 gtggaagagc tcatttcgaa aaaagaggcc aaacattttc ttgaggtcac cggtggaatg
     3361 ctcacgcggt acaatgcgaa aggagaagtg gaatacgagg tgttcgaggc ttccgaagcg
     3421 ttatcggaag gggaagtgct ttcgctcgag gagaaattcc tcgccgggca ggggcagaac
     3481 attacgcctg caaggtattc tgccgatccc gcggaaagac agcagatctc cgcaaaagtg
     3541 aaggcaaccc tcggggctgc ggcgcgtctg ttgaatatcc agggatacac ccggatcgat
     3601 gctttcgtgc gcatttacaa cccggaaaaa gtggaagtga tcttcgtcga agtaaattct
     3661 cttcccggca tgacgccggc cacgtgcatt tttcaccagg ccgcgatcag tcattacaag
     3721 ccgtatgatt tcattgataa aatcctggat ttcggtttcg cacgaaggga ccgtaaaata
     3781 agtcagcccg cctgaccgct ctatgaaaaa gtttttttca ttcttcaaaa gccgcgcatt
     3841 tttcatcaat ctcgggattg cggtgatcct gctgccgctg ctgttttgga tcattttcgc
     3901 gtggatggcg tcgtacaccc ggcacggtga ctttgttgtc gtgcccgatt tcaccaacct
     3961 gaaaattaac cagctcaact ccttcgtcga cgacaaaagc gtgagctacg agatcatcga
     4021 ttccatctgg gacccgaagc tgcagaaagg acttgttctt cgccagatgc cggcgcccgg
     4081 ggcgaaagtg aagcagggca gaaaggttta tctttatgtg acagccacgc atgcgccgga
     4141 gatcgccatg ccgaagctgg ttgatctttc catgcgccag gcaatggcgg tgtgtgaaag
     4201 ctacggactg aaagccgaat tcaggtcgat cgatgatccc tgcgacggct gcatcctgaa
     4261 gcaggaatac aagaaaaagc cgatcaatcc cggcgacccg
//
LOCUS       DASTSF010000140         2499 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_953955,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000140 DASTSF010000000
VERSION     DASTSF010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2499
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_953955"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            124..492
                     /locus_tag="VFU15_04120"
     CDS             124..492
                     /locus_tag="VFU15_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013306938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore germination protein GerW family protein"
                     /protein_id="HEU4716989.1"
                     /translation="MKANLDETIQKLTEFLKDEVKTDTIIGKEFKLGEFSCVPVMSIG
                     LGVGAGGGEGTAQKGDQGEGAGGGAGMGMAPIGFLVTRGEQIQFIAAKGHGALATAFE
                     KLPDLLGKYFESKKKATATA"
     gene            642..2333
                     /gene="sulP"
                     /locus_tag="VFU15_04125"
     CDS             642..2333
                     /gene="sulP"
                     /locus_tag="VFU15_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012505480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate permease"
                     /protein_id="HEU4716990.1"
                     /translation="MKSLESSGAHTRTFMFTPKLFSTLRDYSKAQFGKDLISGIIVGI
                     VALPLAIAFGIASGVSPEKGLITAIVAGFIISAMGGSRVQIGGPTGAFIVIVYGIVQK
                     LGVEGLIISTFIAGVMLIIMGVARLGSVIKFIPHPLIVGFTSGIALIIFSSQVKDFFG
                     LSMGAVPSDFSDKWLAFFHHFSSVNFWSLGIAVLTVVVSFIFPKITHRIPGSLVAILI
                     TTAAVTLFHIPVDTIGSKFGSIPSTLPLPSIPHVDLSVIRQLIQPAFTIALLGGIESL
                     LSAVVADGMIGGSHRSNMELIAQGTANIFSSFFGGIPATGAIARTATNVKNGGRTPVA
                     GIVHSLTLLIIMLFVGKWASLIPMACLAGILVVVSFNMSEYKSFISMLRGPRGDVLIL
                     LTTFFLTVFIDLTIAIEIGMMLSAFLFMQRMAKISKVDVASRYGEKEEVDDPGAIGNY
                     SIPAGTEVFEIKGPFFFGAAYKFKESLRVIENPPKVLIIRMRLVPVIDATGLHALEEV
                     FRFTAKTKTKLIVSGIQPDVKTEFIRSGLMERIGAENVLDDFGMALERAAAVAGR"
BASE COUNT          542 a          729 c          627 g          601 t
ORIGIN      
        1 ctgccgagcc ggatcaattt ttcgggtgca agcacgatca tcattcgtat cgagaacaac
       61 gctttccgtg tgttgaaggc gctctatcta taaaccaaaa taaaaaccaa atccacattt
      121 accatgaaag caaatcttga tgaaaccatt cagaagctca cagaatttct gaaagacgaa
      181 gtaaaaacgg acaccattat cggtaaagaa ttcaagctcg gcgaattcag ctgtgtaccc
      241 gtcatgagca tcggtctcgg cgttggcgca ggcggcggcg aaggaaccgc gcagaaaggc
      301 gaccagggcg aaggcgccgg cggcggtgca ggcatgggaa tggccccgat cggcttcctc
      361 gttacgcgcg gcgagcagat ccagtttatc gcggctaaag gtcacggcgc gcttgcaacc
      421 gcattcgaaa agctgcccga tctcctcgga aaatatttcg aaagcaaaaa gaaggcaacg
      481 gccactgcct gacaggatcg tcacagcggg atccccggaa gatttcctcc cggggattcc
      541 gcatttaatt accaacagcc tgcgggtgag aaagtgacga aagtcatctt ctcccctcct
      601 gcccttttgg ctaatttcgt tcgtctattg ctttacctgt tatgaaaagt ctggaatctt
      661 ccggcgccca tacacggacg ttcatgttca ccccgaagct tttttccacg ttgcgggact
      721 attcaaaagc ccagttcggg aaagacctca tttccgggat cattgtcggc atcgtggcgc
      781 ttccgctcgc catcgcgttc ggcatcgctt ccggtgtttc gccggaaaaa ggcctcatca
      841 ccgccattgt cgccggcttc atcatttccg ccatgggcgg aagccgggtg cagatcggcg
      901 gtcccaccgg tgcgttcatc gtcatcgtat acggcattgt gcagaagctg ggcgtcgaag
      961 gactcatcat ctctacattc atcgccggcg tcatgctcat catcatgggt gttgcgcgcc
     1021 tgggttctgt catcaagttc atcccgcatc cgctcatcgt cggcttcacc agcggcatcg
     1081 cgctcatcat tttttcttcg caggtgaaag atttcttcgg actttccatg ggcgctgtgc
     1141 cttcggattt ttccgacaaa tggctcgcct tcttccatca tttcagctcg gtgaatttct
     1201 ggtcgctcgg cattgccgtt cttaccgtcg tcgtctcctt catttttccg aagatcacgc
     1261 accgcattcc cggctcgctc gtcgccattc tcatcaccac ggcggccgtc acgcttttcc
     1321 atattcccgt agacaccatc ggcagcaagt tcggttccat tccgtccact cttccgcttc
     1381 cgtccattcc ccatgtcgat ctttccgtta tccgccagct catccagcca gcattcacca
     1441 tcgcgctgct cgggggaatt gaatcgctgc tttccgcagt cgtcgccgac ggcatgatcg
     1501 gcggcagtca ccgctcgaac atggagctca tcgcgcaggg aaccgcgaat attttttcct
     1561 cgttcttcgg cggcattccg gcaaccggcg ccatcgcgcg cacagccacg aacgtcaaga
     1621 acggaggacg cacgccggtg gcaggaattg tgcattcgct cacgctgctt atcatcatgc
     1681 tcttcgtggg caaatgggct tcgcttattc ccatggcctg tcttgcaggt atcctcgtcg
     1741 tggtttcttt caacatgagc gaatacaaat cattcatctc catgctccgc ggtccgcgcg
     1801 gcgatgtgct catcctgctc accacgttct tcctcaccgt attcatcgac ctcaccatcg
     1861 ccatcgagat cggcatgatg ctttccgctt tcctgttcat gcagcgcatg gcgaagatca
     1921 gcaaggtaga tgtcgcatca cgttacgggg aaaaagaaga agtggacgat ccgggtgcga
     1981 tcggcaatta cagcattccc gccggaacgg aagtgttcga gatcaaggga cctttcttct
     2041 tcggtgcggc gtacaagttc aaggaatcgc tgcgcgtgat cgaaaacccg ccgaaggtgc
     2101 tcatcatacg catgcgcctc gttcctgtga tcgatgcaac gggactgcat gcactcgaag
     2161 aagttttcag gttcacggcg aaaacaaaaa caaagctcat cgtttcaggc atacagcccg
     2221 acgtgaaaac cgaattcatc cgctcaggac ttatggaaag gataggcgcg gaaaatgtgc
     2281 tcgatgattt cggaatggcg ctggaaagag cggccgcagt ggccgggcgt tagacgccgg
     2341 acgttgtgat aaaagctgac aggaccggct taccctctca cttcccggtt ttgatgaatc
     2401 ttcgccgtga gcaggatgtt ccgttatcca cgacgataaa atacattccg gcattcaggg
     2461 acgatacgtc aacagaaaag tctcctgcat ttctttctc
//
LOCUS       DASTSF010000141         3559 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_508436,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000141 DASTSF010000000
VERSION     DASTSF010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3559
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_508436"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(170..766)
                     /locus_tag="VFU15_04130"
     CDS             complement(170..766)
                     /locus_tag="VFU15_04130"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005837266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEU4716991.1"
                     /translation="MAKRAFDIFFSLAGLLFLLPFFLLIAVFILLDSRGGVFYRQVRV
                     GRYGKDFRLWKFRTMRPDSDKKGLLTVGGRDPRVTRTGYFLRKYKLDELPQLINVLAG
                     DMSLVGPRPEVRKYVDLYSEEQRKVLLAKPGITDYASIDYSEENDLLAGSPDPERAYI
                     EEVMPAKLKLNMKYIEEQSLATDVKIIFRTIGKIAGRK"
     gene            complement(770..1987)
                     /locus_tag="VFU15_04135"
     CDS             complement(770..1987)
                     /locus_tag="VFU15_04135"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEU4716992.1"
                     /translation="MIPFSPPRIDQKIIDEVTATLRSGWITTGPKTKLFEKKLAEYCG
                     AKSVLCLNSATAGLEIMLRWFGVGEGDEVILPAYTYAATANVIIHCGAKPVFVDVSEK
                     DFNISVKAIENAVTEKTKVIIPVDFGGWPCDYNAINALAVKMKDKFRPSSKEQEMLGR
                     MLVLSDAAHSIGAVYKGKKTGALTDVSVFSFHAVKNLTTAEGGAVALNLPEPFDNAGL
                     YNLLCVKTLHGQNKDALAKTQKGSWKYDIVEAGYKCNMTDILASIGLVELERYDNDTL
                     VKRKKIVDKYSWVLSAYSWAELPLMDDEKQNTHSSYHLFPLRIKGITEEQRDQIIRKI
                     FEKEVSVNVHFIPVPMMSFYRNLGYSIQDYPKTYGNFSREISLPVYYDLSEEQALVVL
                     EAVASSVIEVKGS"
     gene            complement(1984..3198)
                     /locus_tag="VFU15_04140"
     CDS             complement(1984..3198)
                     /locus_tag="VFU15_04140"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEU4716993.1"
                     /translation="MKTKIDNVRTGFICAAAALLPVNFFAVSMITIVILILTVWIFFR
                     EKRKPAPLKLLLPVLIFAFTLIGMIWSAEGSSSIVTVKFSWLIFPIIFFLVPLSGKTE
                     TAAVKTSFLGGLLVSTFVCTWWAFTRYHVSRNPADLFYTGFSMFMHPSYYAMYVTAGC
                     IFLWEKIMGNGNGMRMQLVSFVLLLWFGFVLVMLQSKAGIIVSLSLVGIMGLVYSWKT
                     KKMITGAIGLAAFYFLFWAATRYVVTSNNSRIFNAEYNVTHFTAQAEKAPESSQMRIL
                     VWQSDWELIRSNFLAGVGSGDVVTELKKVYHRRGITKAEEEGLNAHNQYFQSWLEAGI
                     GAFVALLAALAVPGLTAVRKKDLVLGLFLAAIILNFGVESMLCTQAGTMFTAFFLSFL
                     FSAAETKEELPV"
     gene            3268..>3559
                     /locus_tag="VFU15_04145"
     CDS             3268..>3559
                     /locus_tag="VFU15_04145"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU4716994.1"
                     /translation="MKNFFKRIDDFFFGHLQMHYARSLEKECEGYESLLDIGCGSDSP
                     VKLFSKKMKRVTGIDGFQPSIDKARAAGIHTEYYLMNVMDVAEKFPPHSFDVV"
BASE COUNT          887 a          957 c          894 g          821 t
ORIGIN      
        1 cgagcgattg aaatacgacc tgctctatat cgagaacatg tcgctttacg tcgatttcaa
       61 gatcatgatc tatacgctgc tgatcgttat ccagggacgc ggcaagtaat ctattggcaa
      121 ggacgaatga ttaagaatgg cgggagaaat acggcccgct catcattcat catttacggc
      181 ccgcaatttt cccgatcgtc ctgaaaataa ttttcacatc cgtggcgaga ctctgctctt
      241 cgatgtattt catgttcagc ttcagcttgg ccggcatcac ctcttcgata tacgcccgct
      301 cgggatcggg tgatccggca agaagatcat tctcttccga ataatcgatg gacgcataat
      361 ccgttatccc gggttttgcc agcagcactt tccgctgttc ttccgaatag agatcgacgt
      421 atttgcgcac ttccggccgt ggcccgacga ggctcatatc gccggcaagc acattgatca
      481 gctggggaag ctcatcgagc ttgtatttgc ggagaaaata ccccgtgcgc gtcacccgcg
      541 gatcacgtcc acccacagtg agcaatcctt ttttatcgga atcaggacgc atcgtgcgga
      601 atttccagag gcggaaatct tttccgtaac ggcccacgcg gacctgccgg taaaaaacac
      661 cgccgcggct gtcgagcagg ataaaaacgg cgatcagcag gaaaaaagga agcaggaaaa
      721 gaagtcccgc caatgaaaaa aaaatatcga acgcgcgctt tgccaccgct cagcttcctt
      781 tcacttcaat gacagacgaa gccaccgctt cgagcacgac cagcgcctgt tcctcggaca
      841 ggtcgtaata cacgggaagc gaaatctcgc gtgaaaaatt cccgtatgtc ttcgggtaat
      901 cctggatgct gtaaccgagg ttgcggtaaa agctcatcat cggaaccggg atgaaatgca
      961 cgttcacgct cacctccttt tcaaaaatct tccggatgat ctggtcgcgc tgctcctcgg
     1021 tgattccctt tatgcgcagg ggaaatagat ggtaggacga atgcgtgttc tgtttttcat
     1081 catccatcag cggaagctcg gcccaactgt acgcggacaa tacccagctg tatttatcga
     1141 cgatcttttt ccgtttcacc agcgtgtcat tgtcgtaacg ttccagctcc acgagaccga
     1201 tcgaagcgag aatatccgtc atgttgcact tgtagcccgc ttccacgatg tcgtatttcc
     1261 agcttccttt ctgcgttttg gccagcgcat ccttattctg cccgtgcagc gttttcacgc
     1321 aaagcaggtt atagagcccg gcattgtcga acggttccgg gaggttgagc gcaacagcac
     1381 cgccttccgc ggtggtgagg tttttcacgg cgtgaaacga aaatacgctc acgtcggtca
     1441 gggcaccggt tttctttcct ttgtaaacag cgccgatgga atgagccgcg tcgctgagca
     1501 cgagcatgcg gccgagcatt tcctgctctt tcgacgacgg acggaatttg tctttcattt
     1561 tcaccgcgag cgcgttgatc gcattgtaat cgcagggcca tccgccgaag tcgaccggta
     1621 tgatcacttt tgttttttcc gtgacggcgt tctcgatcgc tttgacgctg atgttgaaat
     1681 ccttttcact cacatccacg aaaaccggtt tcgcaccgca atgaatgatc acgttcgccg
     1741 ttgccgcata cgtgtatgca gggaggatca cctcgtcgcc ttcgccaacg ccgaaccagc
     1801 ggagcatgat ctcgagccct gccgtcgctg aattcaggca cagcaccgat ttcgcgccgc
     1861 aatattccgc cagttttttt tcgaacagct ttgttttggg cccggtggtg atccaacccg
     1921 aacgcagcgt agctgttact tcatcaatga tcttctgatc gattctcgga ggagaaaaag
     1981 gtatcataca ggaagttctt cttttgtttc cgcagctgaa aaaaggaacg aaagaaaaaa
     2041 tgcggtgaac atcgttcctg cctgcgtgca gagcatgctt tcgacgccaa aattcaggat
     2101 aatggcggca aggaacaagc cgagcaccag gtctttttta cggaccgccg tgaggcccgg
     2161 caccgcaagc gcagcgagca gcgcgacaaa cgcgccgata ccggcttcca gccacgactg
     2221 gaaatactgg ttgtgcgcgt tcagtccttc ttcttcggct ttcgttatgc cgcgccggtg
     2281 ataaaccttt ttaagctcgg tgaccacatc gccggaaccc acgccggcga ggaaattcga
     2341 acggatcagt tcccagtcgc tttgccacac gaggatgcgc atctggctcg attcgggcgc
     2401 tttctccgcc tgtgccgtga aatgcgtcac attgtattcc gcgttgaaaa tgcgtgaatt
     2461 gttcgaagtg accacgtaac gtgtcgcggc ccagaaaaga aaataaaatg ccgcaagacc
     2521 gatcgcgccg gtgatcattt tttttgtctt ccagctatag accagcccca ttattcccac
     2581 gagcgagagc gaaacgataa taccggcttt cgactgcagc atgacgagca cgaagccgaa
     2641 ccagagcagc agaacgaaag agacgagctg catgcgcatc ccgtttccgt ttcccatgat
     2701 cttttcccag agaaaaatgc atcccgcggt gacgtacatc gcgtaatacg aaggatgcat
     2761 gaacatggaa aagcccgtgt aaaaaagatc cgccgggttc cgcgaaacat ggtaacgcgt
     2821 gaatgcccac caggtgcaca cgaaagtcga aacgaggagt ccgccgagaa aagaagtttt
     2881 caccgcagcg gtttccgtct tccctgaaag cggaacgaga aagaaaataa tgggaaatat
     2941 cagccaggaa aattttaccg tcacgatgga agaagaacct tccgccgacc agatcatgcc
     3001 gatgagcgta aaggcgaaga tgagcacggg caaaagaagc ttcagcgggg ccggctttct
     3061 tttttccctg aaaaatatcc acaccgtcag gatgagaatg acgatggtga tcatggaaac
     3121 ggcgaaaaaa ttgaccggga gcaaagccgc ggctgcgcag ataaaaccgg tgcggacgtt
     3181 atcgattttt gtcttcaacc gcgttaaata tacacagatt caggccataa aatctttgta
     3241 cattcgcgcg catcagcagc gaacatcatg aaaaatttct ttaagcgcat cgatgacttt
     3301 tttttcgggc acctgcagat gcattatgcg cgctcgctcg aaaaagaatg cgaaggatat
     3361 gaatcgctgc tcgacatcgg ctgcggttcc gattcgccgg tgaagctgtt ctcaaagaaa
     3421 atgaaacgcg tgaccggcat agacggattc cagccttcca tcgacaaagc ccgggccgcc
     3481 ggcatccaca ccgaatatta cctgatgaac gtaatggacg tcgcggaaaa attcccgccg
     3541 cattcattcg acgtcgttg
//
LOCUS       DASTSF010000142         4633 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1176067,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000142 DASTSF010000000
VERSION     DASTSF010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4633
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1176067"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..917
                     /locus_tag="VFU15_04150"
     CDS             <1..917
                     /locus_tag="VFU15_04150"
                     /inference="COORDINATES: protein motif:HMM:NF012892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEU4716995.1"
                     /translation="NILTGKQLYHMTDDALAACVEDVSIFAETEPAQKERIVRALQKA
                     GNTVGYLGDGINDASAIKVADVGISVSNAVDVAREAAGIVLLEKNLDVLSDGIEEGRK
                     TYLNTLKYIFLTTSASFGNMFSMAVASFLIPFLPLLPVQVLLLNFLTDLPSFAIASDN
                     VDAEMLAKPRKWDMKLIRNFMIVFGLESSIFDFITFSLLYFSFHAGQDLFRSGWFVES
                     ALTEVLILMIIRTRRPFVKSLPGKLLLSASLAVVAAVIVIPYTLQGNVFSFVPLPAAV
                     IAAMIGIALLYTVTGEMTKRILFRKLHF"
     gene            926..1531
                     /locus_tag="VFU15_04155"
     CDS             926..1531
                     /locus_tag="VFU15_04155"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEU4716996.1"
                     /translation="MNNDHLTHLILVYSYAGIFTWFVFFDQLIPLPEEAALLSIGYLA
                     AQVPLNIFFCMLSSVVALLLIDNFYYYLTKRGSRLTERMKKKLGEHTRAKTQAYMNRN
                     STWTLFGLALLPRIRFFAPMLAAGFNVSWKKFAIVNSAATVLYVLVYTLLGYVFHNRL
                     DKLMHETELLRHIVFIVAVIVIVGVASFVVTKKLMRNGKKV"
     gene            complement(1534..2091)
                     /locus_tag="VFU15_04160"
     CDS             complement(1534..2091)
                     /locus_tag="VFU15_04160"
                     /inference="COORDINATES: protein motif:HMM:NF012345.4"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEU4716997.1"
                     /translation="MIAIIDFGSRKTPLIAEMVSACGKENALIGWNDFSLEKIASSSA
                     IIFSGSPAMFTEADRTPYFEKFNFIKEGRIPVLGICFGHQLLGLLHGATIFRGKEITE
                     TIRIKVLKKDPLFEGLFPETEMKEDHTEGITLPPGFVHLATSSTYTVEGMRHPFLPLW
                     GVQFHPEVSGENGKKLVGNFCSLVS"
     gene            complement(2101..3348)
                     /locus_tag="VFU15_04165"
     CDS             complement(2101..3348)
                     /locus_tag="VFU15_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009185035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase family protein"
                     /protein_id="HEU4716998.1"
                     /translation="MEHKFSPESLMMSYGYKPELSEGAIKCPIFQTSTFVFKTAEEGK
                     SFFELAYGLRDKSPGEELGLIYSRINNPDLEILENRLCLWDGADDCAVFESGMSAITT
                     VLLEFLKPGDLLLFSTPVYGGTDHFIHHFLPKIGVDAIGFKPGMSEEEIISLLKNSGK
                     PGNLAMIYIETPANPTNTLIDIEECRRIADAFSTKEKRVLIATDNTYMGPLWQHPLKH
                     GSDLVVYSATKYIGGHSDVIAGAVLGNKPLMQRVKTLRTFLGNMAGPWTGWLLLRSLE
                     TLKVRMTQQAENAMQVAKFLRNHPKVEKVYYLGLIPEGTSEYEIYRRQYSSPGAMLSF
                     DVRGGEKAAFRFLNGLKLIKLAVSLGSTESLAEHPASMTHAGVLPEHRADMGIGDSMV
                     RLSVGVENYADIIYDIGNALEKV"
     gene            complement(3483..>4633)
                     /locus_tag="VFU15_04170"
     CDS             complement(3483..>4633)
                     /locus_tag="VFU15_04170"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEU4716999.1"
                     /translation="KDRDNDKVSDKLDKCKDVAGVWEFMGCPDKDGDHVQDSEDLCPD
                     VPGLAKFNGCPDRDNDGIVDSQDACPDDPGLPQFNGCPDKDGDGIIDKDDDCPDEKGL
                     VQFKGCPDRDGDGVMDKIDLCPDKPGPVDNDGCPEVILNLVDLAGQSLKSAKQAKDGS
                     FTFDNLPSDSICVFRLDGDPDKTIGVNEVKVIVNGLPKRAIRSQADGLFRFDIPKPVG
                     NGLQKVEVKDVAVTLSQADQAIVKKAFDNLEFETGKDIIRTSSYAALDSLAYLLKKHP
                     EWGLKISGHTDNVGNPVANMTLSKKRAEAVKKYLVSKGVAADHLKTEWYGQTRPIAPN
                     TTAEGRQKNRRVEMLIVDYYKGMDAPFVPAKAPAKTPAKTPAKKPVKK"
BASE COUNT          931 a         1241 c         1274 g         1187 t
ORIGIN      
        1 ccaatattct cacggggaaa cagctgtatc acatgacgga tgatgcgctg gccgcgtgcg
       61 tggaggacgt cagcatattt gcggaaacag aaccggcgca gaaagagcgc atcgtgcgcg
      121 cgctgcagaa agccgggaac acggtaggtt acctgggcga cggcatcaac gacgcttccg
      181 cgatcaaagt ggcggatgtg ggcatttccg tgagcaacgc tgtggatgtt gcgcgcgaag
      241 cggcgggcat cgttctgctc gaaaaaaatc tcgatgtgct cagcgatgga attgaagaag
      301 gaagaaaaac atatctcaac acgctcaaat atatttttct caccacgagc gccagcttcg
      361 gcaatatgtt cagcatggcg gtggcgtcgt tcctcatccc gttcctcccg ctgctcccgg
      421 tgcaggtgct gctgctgaat tttctcacgg atcttccttc gtttgcgata gcgtcggata
      481 atgtcgatgc ggaaatgctc gcgaagccgc ggaagtggga catgaaactc atccggaatt
      541 tcatgatcgt cttcggtctc gaaagctcga tcttcgattt catcacgttc tctctcctgt
      601 atttcagctt tcacgccggg caggatcttt tccgcagcgg gtggttcgtg gagtcggcgc
      661 tgaccgaagt gctcatcctg atgatcatcc gcacgagaag accgttcgtg aaaagtcttc
      721 ccgggaagtt attgctgtcg gcgtcgctgg ccgtggtcgc tgcggtgatc gtcattccgt
      781 atacgctgca gggaaacgtg ttcagctttg ttccgctccc ggctgcggtg atcgcggcga
      841 tgatagggat tgcgctgctt tataccgtca ccggtgaaat gacaaaacgt atattattcc
      901 ggaagctgca tttctgatct gaaaaatgaa caacgatcac ctcacacatc tcattctcgt
      961 ttattcctac gcagggattt ttacgtggtt cgtttttttc gaccagctga tcccgctgcc
     1021 ggaagaagct gccctgctca gcatcggtta cctggctgca caggttccgc tgaatatttt
     1081 cttctgcatg ctgtcgtcgg tggtcgcgct cctgctgatc gacaactttt attattacct
     1141 gacgaagcgc gggagccggc tcacggagcg catgaagaaa aagctgggcg agcatacgcg
     1201 cgcaaaaaca caggcgtaca tgaacaggaa cagcacgtgg acattgttcg gtcttgcgct
     1261 gctgccgcgg atacggtttt tcgccccgat gctggctgca gggttcaatg tgtcatggaa
     1321 aaaattcgcg atcgtgaaca gtgcggccac cgtgctgtat gtgctggtgt atacgctgct
     1381 cggttatgtt ttccacaacc ggctggataa gctcatgcac gaaacagaac tcctgcgtca
     1441 catcgttttc attgttgcgg tgatcgtgat cgtcggggtg gcttcattcg ttgtgacgaa
     1501 gaagctgatg cggaacggga agaaagtatg agctcaggaa accagcgagc agaaattacc
     1561 gaccagtttt tttccattct ctcccgaaac ttccgggtga aactgcacgc cccacagggg
     1621 aagaaacggg tgccgcatac cctcgaccgt gtacgtggag gacgtggcga ggtggacaaa
     1681 tcccggcgga agcgtgatgc cctcggtgtg atcttccttc atttcggttt cgggaaaaag
     1741 tccttcgaag agcgggtctt ttttcagcac ttttatgcgg atggtttcgg tgatctcttt
     1801 cccgcggaag atcgtcgcgc catgcagcaa tcccagcagc tggtgaccga aacagatccc
     1861 gagcacggga attcttcctt ctttgatgaa attgaacttt tcaaaataag gcgtgcgatc
     1921 cgcttcggtg aacatcgccg gcgatccgct gaaaatgatc gcggaagaag acgctatttt
     1981 ttccagagaa aaatcgttcc agccgataag ggcattttct tttccgcagg cgctcaccat
     2041 ctctgcgatg agcggtgttt tgcggctgcc gaagtcgatg atcgcgatca ttcaggaaaa
     2101 ttacactttt tccaatgcgt tgccgatgtc gtaaatgatg tccgcgtaat tctccacacc
     2161 gacggacaaa cgcaccatgc tgtcgccgat gcccatgtcg gcgcggtgtt cgggcaggac
     2221 gccggcatgc gtcatggaag caggatgttc cgccagcgat tccgtgctgc cgaggctcac
     2281 ggcgagcttg atcagcttca gcccgttgag gaaacggaac gccgcttttt ccccgccgcg
     2341 cacgtcgaac gaaagcatcg cgcccggcga agaatattgc ctgcggtaaa tttcatattc
     2401 ggaagttcct tcaggaatga gcccgaggta atatactttt tccactttcg gatgattgcg
     2461 caggaacttc gccacctgca tcgcattttc ggcctgctgc gtcatgcgca ctttcagcgt
     2521 ttcgagactg cgcaggagga gccagccggt ccacggtcct gccatgttgc cgaggaaagt
     2581 gcgcagcgtt ttcacgcgtt gcatgagcgg tttgttcccc agcactgcgc ccgcgatgac
     2641 gtcgctgtgc ccgccgatgt attttgtggc ggaataaacg acgaggtcgc tgccgtgctt
     2701 cagcgggtgc tgccacagcg gacccatgta cgtattgtcg gtggcgatga gcacgcgttt
     2761 ttcttttgtg ctgaacgcat cggcgatccg ccggcattct tcgatgtcga tgagcgtgtt
     2821 cgtcgggttg gcgggcgttt cgatgtagat cattgccagg tttcccggtt tgcctgaatt
     2881 tttcagcagg gaaattattt cctcttcgct catgcccggc ttgaagccga ttgcgtccac
     2941 gccgatcttc ggaagaaagt ggtgaatgaa atgatccgtt ccgccgtata ctggcgtaga
     3001 gaacagcagc aggtcgcccg gcttcaggaa ttcgagcagg acggttgtga tggccgacat
     3061 gccgctctcg aaaaccgcgc agtcatccgc tccgtcccac agacataacc ggttttccag
     3121 tatttcaaga tcgggattgt tgatgcggct gtaaatgagt cccagctctt cacccggcga
     3181 tttgtcacgc agcccgtaag cgagttcgaa aaaacttttt ccttcttccg ccgttttgaa
     3241 aacgaaggtc gacgtctgga agatcgggca tttgatcgcg ccttcggaaa gttccggctt
     3301 atagccgtag gacatcatca ggctttccgg tgaaaatttg tgttccattt cctgctgttt
     3361 tttttatccg tctaaaagta ggcggctttt tcttctttca ccgtgacggc gatcagtaaa
     3421 atgcgaacaa aaaaagaccc ggatcgctcc gggcctttgt cttacgtctc acgcttgttt
     3481 ttttacttct tcaccggttt tttcgccggg gtttttgcag gcgttttcgc cggggccttt
     3541 gcgggaacga acggagcatc catgcctttg tagtaatcca cgatcagcat ctccacgcgg
     3601 cggtttttct gtcttccttc cgctgtcgtg ttcggtgcga tcgggcgtgt ctgtccgtac
     3661 cattccgtct tgaggtgatc agctgcaacg cctttcgaca caagatattt cttcacggct
     3721 tccgcacgtt ttttcgagag cgtcatattc gcaacgggat tacccacatt gtccgtgtgg
     3781 ccgctgatct tcagtcccca ctcagggtgt ttcttcagaa ggtaagcgag cgaatcgagc
     3841 gcagcatacg agcttgtgcg gatgatgtct ttgccggttt cgaactcgag gttgtcaaac
     3901 gctttcttca cgatcgcctg gtcggcctgc gaaagcgtaa cagcgacatc tttcacttcc
     3961 actttctgga gaccgttgcc cacaggtttc ggaatatcga aacggaagag tccgtcggcc
     4021 tggctgcgga tggcgcgctt gggcaatccg ttcacgatca ctttcacttc attcacaccg
     4081 atcgttttgt caggatctcc gtcgaggcgg aacacacaga tcgaatccga aggcaggttg
     4141 tcgaacgtga agcttccgtc tttcgcctgc ttcgctgatt tcaggctttg tcctgcaagg
     4201 tccacgagat tgagaatgac ttccgggcag ccgtcgttgt cgaccgggcc gggcttatcg
     4261 gggcaaaggt cgatcttgtc catcacgccg tcaccgtcac ggtcggggca gcctttgaac
     4321 tgcacgagac ctttttcatc cgggcagtcg tcgtctttgt caatgatgcc gtcaccgtct
     4381 ttatccgggc agccgttgaa ctgcgggaga cccggatcat ccgggcatgc atcctggctg
     4441 tcgacgatgc cgtcgttgtc gcggtcggga catccgttga atttcgcgag acccggaaca
     4501 tccgggcaaa ggtcttccga atcctgcacg tggtcgccgt ccttatccgg gcagcccatg
     4561 aattcccaaa cacccgcgac gtctttgcac ttgtcgagct tgtcggagac tttatcgttg
     4621 tcacggtctt tcg
//
LOCUS       DASTSF010000143         4335 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_57886,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000143 DASTSF010000000
VERSION     DASTSF010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4335
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57886"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            174..3278
                     /locus_tag="VFU15_04175"
     CDS             174..3278
                     /locus_tag="VFU15_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009284056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HEU4717000.1"
                     /translation="MVNFMKGIIAFSLKNKLFILFMTGLTVVAGLISYFNTPVEAFPD
                     VTNTQIEIITLWPGRSCTEVEKFVTIPIEIAMNSVQKKTIVRSISMYGLSDVIIIFDD
                     GVDDPFARQQVNNLIHEVELPEGLEPEVLPPGGPTGEVYRYTVQSPKRNIRDLTTIQN
                     WTIDRNLRAVPGVADIVTFGGDIKTYEIQVDPHLLAKYDLTPLDVYDAVSKSNINVGG
                     DIIEKNEQAYVVRGIGLLTSIPDIENIIIKENGGTPILVKNVATVVESALPRLGSVAL
                     DTSKDVVEGVVIMRKGENPGEFLHNLKEKIKQLNNGILPPDVKIVPFYDRDKLIGFCT
                     HTVLHNLFEGIILVTLVVFVFMADWRTTVTVAVIVPMALLFAFICLRIMGMTANLLSM
                     GAIDFGIIIDGAVVMVEGIFVALDHKAHAIGMDRFNRLAKLGIIRRTGAEMGKAILFS
                     KIIILTALIPIFSFQKVEGKMFSPLAFTLGFALLGALIFTLTLVPVMSSMLLKKNVRE
                     KNNPFTNGITKLFIGMFSAAFRRKTLSLALALIMLAGSIFCFRFLGTEFLPDLNEGAL
                     WVESSLPLSVSITESRKIADKMRGILMKFDEVDGVLSQTGRPADGTDPDGFYSIQCQV
                     NLKPKDEWKTEMSSDELIDSMDAQLRQIPGVIFNYSQPIIDNVHEAVAGMNADIAVKV
                     YGPNLKTIDSIGRQIIPIMKNVRGIEDVGLLKTIGQPELLISLDQARMKAYGVSTGDA
                     QAVVEMAIGGKAATQLYDEDRRFDIRIRYAPQYRSDETQIGNLMVPTLTGGKVPLKAI
                     ATIKLETGDIRISRDNNTRFGGVKFTIRDRDMGSAIKEAQDSINAKLKLPEGYHLEWA
                     GEYENQVRATKRLTQVVPISLVIIFVILFIAFSNIKDAVIVIVTVPFSLIGGILALLL
                     THTIFGISAGIGFIALFGVCVQNGVILISVFKKNLANKMSLRDAITAGVASRVRPVVM
                     TAMMAAIGLLPAAISTGIGSETQKPLAIVIIGGLVSATVLTLLVLPIIFERAYKRKHS
                     ELGI"
     gene            3303..4022
                     /locus_tag="VFU15_04180"
     CDS             3303..4022
                     /locus_tag="VFU15_04180"
                     /inference="COORDINATES: protein motif:HMM:NF022142.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2490 domain-containing protein"
                     /protein_id="HEU4717001.1"
                     /translation="MKTKAFAFQLTVTFLVSGALFSAGSCFAQSASAKANNDAQLWEN
                     LNVNKKLTQNFTLRFSHEGRFTQNDSRLTYYFGDFGAEYEIPKTHFGILLDYVFIRKE
                     LPEYWSTRHQFYTALTYGKKFGKFSVHDRQMFLGQVKDVNTSIDGRYPEWYVRNKLTL
                     KYKFNFFWSAYVAEELYWHINIPRPDALPHFNRVRYFAGLFFKPDNHNEFEVYYLIEQ
                     HMNTTAPPHNFVLGVGYELSL"
     gene            4209..>4335
                     /locus_tag="VFU15_04185"
     CDS             4209..>4335
                     /locus_tag="VFU15_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010599059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrophosphatase"
                     /protein_id="HEU4717002.1"
                     /translation="MTISEAQDIVDRWIKKHGVRYFDQLTNTAILMEEVGEVARIM"
BASE COUNT         1036 a         1212 c         1194 g          893 t
ORIGIN      
        1 tctatgtggt cgtaaaaaaa accaccactg aattcgagaa gagggagatc attccgggtc
       61 acacacgcga gaagaaaacg tacgtgaaat cggggctgaa aagcggggag atcgtggtga
      121 ccgacggcag tcttcttgca gccaacaaca aactttaagc gggaggattt tctatggtca
      181 atttcatgaa aggcattatc gccttctcgc ttaagaacaa actgttcatc ctgttcatga
      241 cgggactcac ggtcgttgcg gggctgatca gctatttcaa cacgccggtg gaagcgttcc
      301 ccgacgtcac caacacgcag atcgagatca tcacgctctg gccgggaaga agctgcacgg
      361 aagtagaaaa gttcgttacg atcccgatcg agatcgcgat gaattccgtc cagaaaaaaa
      421 ccatcgtccg ctcgatctcc atgtacggcc tttccgacgt gatcatcatt ttcgacgacg
      481 gcgtggatga tccgttcgca cgccagcagg tgaacaacct catccacgaa gtggagcttc
      541 ccgaaggcct cgaaccggaa gtgcttccac ccggcggacc taccggcgag gtttaccgct
      601 acaccgttca gagtccgaaa cgaaacatac gcgacctgac gacgatccag aactggacga
      661 tcgacaggaa cctgcgcgct gttccgggcg tggcggatat cgtcacgttc ggcggagata
      721 tcaagaccta cgagatacag gtggacccgc accttcttgc gaaatacgat ctcacgccgc
      781 tcgatgttta cgatgcggtc agcaaaagca atatcaatgt cggcggggac atcatcgaga
      841 agaacgagca ggcgtacgtc gtacgcggga tcggtctgct gacaagcatt cccgatatcg
      901 agaacatcat catcaaggag aacgggggga ctcctatcct cgtcaaaaat gtcgcgaccg
      961 tcgtcgaatc tgcgctgccg cggctgggtt ccgtggcgct ggatacttcg aaagacgtgg
     1021 tggagggcgt cgtcattatg cggaagggag aaaaccccgg cgagttcctg cacaacctga
     1081 aagaaaagat aaagcagctg aacaacggca tacttcctcc ggacgtgaag atcgttccat
     1141 tctacgaccg cgataaactg atcgggttct gcacgcatac cgtgctccac aacctgttcg
     1201 aaggcatcat cctcgtcacg ctcgtcgtat tcgtcttcat ggccgactgg cgaacgacgg
     1261 tcaccgtagc cgtcatcgtc ccgatggcgc tgctgttcgc gttcatctgc ctgcggatca
     1321 tgggcatgac ggccaacctg ctttccatgg gcgcgatcga tttcgggatc atcatcgacg
     1381 gcgcggtcgt gatggtagag ggaattttcg tcgcgctcga tcacaaggcg cacgccatcg
     1441 ggatggaccg gttcaacagg ctggcgaagc tcggcatcat ccgcagaacc ggcgctgaaa
     1501 tgggcaaggc cattcttttc tcgaagatca tcatccttac ggcgcttatt cccattttct
     1561 cattccagaa agtggaagga aaaatgttct ctccgcttgc gttcacgctc ggctttgcgc
     1621 tgctcggcgc gctgatcttc acacttaccc tggtgccggt tatgtcgagc atgctgctga
     1681 agaaaaacgt gcgcgagaag aataacccgt tcaccaacgg gatcacaaaa ttgttcatcg
     1741 ggatgttctc ggcggctttc cggcgcaaaa cgctcagcct tgcgctggcg ctgatcatgc
     1801 tggccggaag tattttctgc ttccgtttcc tcggcacgga attcctgccc gatctcaacg
     1861 aaggcgcgct gtgggtggaa tcgagcctgc ccctgagcgt ttctattacg gaatcacgca
     1921 agatcgccga caagatgcgc ggcatactaa tgaagttcga tgaggtcgac ggcgtgcttt
     1981 cgcaaacagg acgccccgca gacggaaccg atcccgacgg attctacagc atccagtgcc
     2041 aggtaaacct gaagccgaaa gacgaatgga aaacggaaat gtcttccgac gagcttatcg
     2101 acagcatgga cgcgcagctg cggcagatac ccggcgtgat cttcaactat tcgcagccga
     2161 tcatcgacaa cgtgcatgaa gcagtagcgg gaatgaatgc ggacatcgcc gtgaaggtgt
     2221 acggaccgaa cctgaaaacg atcgattcga tcggaaggca gatcatcccg atcatgaaaa
     2281 acgtgcgcgg catcgaagac gtcggcctgc tgaaaacgat cggccagccc gagcttctca
     2341 tttccctcga ccaggcgcgc atgaaagctt acggcgtgag cacaggcgac gcgcaggccg
     2401 tggtggaaat ggcgatcggt ggaaaagcgg cgacgcaatt gtacgacgaa gaccggcgct
     2461 tcgacatccg gatccgttat gcgccgcaat accgttcgga tgaaacgcag atcgggaacc
     2521 tgatggtgcc cacgcttacc ggcgggaaag ttccgctgaa agcgatcgca acgataaagc
     2581 tggaaaccgg cgacatacgc atttcgcgcg acaacaacac gcgtttcggc ggcgtgaagt
     2641 tcacgatacg cgaccgcgac atgggcagcg cgatcaagga agcgcaggac agcatcaacg
     2701 cgaagctgaa gctgcccgaa ggttatcacc tcgagtgggc aggcgaatac gagaaccagg
     2761 tccgcgccac gaaacgtctt acacaggtgg tgcccatcag cctcgtgatc atcttcgtca
     2821 tcctgttcat cgcgttctcg aacatcaagg atgccgtgat cgtgatcgtg acggttccgt
     2881 tctcgctcat cggcgggatc ctggcgctgc tgctcacgca cacgatcttc ggcatttcgg
     2941 ccggcatcgg cttcattgcg ctgttcggcg tgtgcgtgca gaacggcgtg atcctgatat
     3001 ccgtgttcaa gaagaacctg gcgaacaaaa tgtcgcttcg tgacgcgatc acagcaggcg
     3061 tagcttcacg cgttcgcccg gtggtcatga cggccatgat ggcggcgatc ggcctgcttc
     3121 cggcggccat ttcaacgggc atcggctccg aaacgcagaa gccgctggcg atcgtgatca
     3181 tcggcggact cgtcagcgca acagtgctta cactgctcgt gttgccgatc attttcgaac
     3241 gcgcctacaa acgcaagcat tccgagctcg gaatataaac caggtttaaa aagccgctgt
     3301 ccatgaaaac aaaagcattc gcattccagc ttacggtgac attcctcgta tccggcgcgc
     3361 tgttttcagc gggcagctgt tttgcccaat ccgcttccgc gaaagcgaac aacgacgcgc
     3421 agctatggga aaacctgaac gtgaacaaaa aactgacgca gaatttcacg ctgaggttca
     3481 gccacgaagg ccggttcacg cagaatgatt cccggctcac gtattatttc ggcgacttcg
     3541 gcgcggaata cgagatcccg aaaacgcatt tcggcattct tctcgattac gttttcatcc
     3601 gcaaagagct gccggaatac tggagcacgc gccaccagtt ctacactgcg ctgacgtacg
     3661 ggaaaaaatt cgggaagttc agcgtccacg accggcagat gttcctcggg caggtgaagg
     3721 acgtgaacac cagcatcgac gggcgttacc ccgaatggta cgtgcgcaac aagcttacgc
     3781 tgaagtacaa attcaatttc ttctggtcgg cgtacgtggc ggaagaactg tactggcaca
     3841 ttaacattcc ccggccggac gcgctgccgc atttcaaccg cgtacggtat ttcgccgggc
     3901 tgttcttcaa gccggacaac cataacgaat tcgaggtgta ttacctcatc gaacagcaca
     3961 tgaatacaac agctcctccg cacaacttcg tgctcggcgt cggctacgag ctttcgcttt
     4021 agtacgaaga agtgaacggc tgccgggatt tctgacaggg ttttctccgg cggccgggag
     4081 ggtgctggat ttcggatttc gcatgtcgga tgccggattt cggatgccgg atttgaatcc
     4141 atcctcattg cttcatcctg ttccggccaa tccactcctt tccttacatt cgggcaaaac
     4201 aaacgatcat gaccatcagc gaagcccagg atattgtcga ccgctggata aagaagcacg
     4261 gcgtgcgcta tttcgaccag ctcacgaata cggcgatact catggaggaa gtcggtgaag
     4321 tggcccgcat catgt
//
LOCUS       DASTSF010000144        17908 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_164474,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000144 DASTSF010000000
VERSION     DASTSF010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 17908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17908
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164474"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="VFU15_04190"
     CDS             <1..216
                     /locus_tag="VFU15_04190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA
                     isomerase"
                     /protein_id="HEU4717003.1"
                     /translation="DEAMKIAVTISQMPTKAIGLTKRLLNEGTVNSLDEQLVREGEEQ
                     VAATRTYDFTEGVKAFLEKRKPEFRGE"
     gene            283..1449
                     /locus_tag="VFU15_04195"
     CDS             283..1449
                     /locus_tag="VFU15_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018128007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEU4717004.1"
                     /translation="MDQHTVVGVIGSGAMGSGIALVAATAGHKVFLYDNNAKQLAKAE
                     ETLLQLLKRIVEKGKLRQEDADTIFKRLVFSAEMSSFSECGLVIEAIVENIDVKQKVF
                     SDLENIVGESCVLASNTSSLSITSIASACKKTGRVIGIHFFNPAPLMPLAEIIPGIST
                     DRNILAACKTLISGWGKSVVQAKDTPGFIVNRVARSFYGESIRIYEEGIADFATIDWA
                     MKTYGGFKMGPFELMDFIGNDVNYKVTETVWEQFFYEPRFKPSLTQKRLYEGKMFGRK
                     SGQGYYDYREGAVMPEPVKDETLGKAIFFRVLVMLINEAADSLYLGLATREDIDTAMT
                     KGVNYPKGLLRWADETGIAAVTGKLSSLYEEYGEDRYRPCVLLKRMTKEGKTFY"
     gene            complement(1466..1885)
                     /locus_tag="VFU15_04200"
     CDS             complement(1466..1885)
                     /locus_tag="VFU15_04200"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU4717005.1"
                     /translation="MKKETKLLLVDDDDVFIFITKKTIDESRLVGEVNVCANGMDAIR
                     FLEKLLPEPEKLPDVILLDLNMPVLDGWGFLAEYETLRKKLPGKIDLYVVSSSVSPHE
                     VERAKSFSYVSDFIVKPVTAETIGLIRERLTRQEGNN"
     gene            complement(1882..3684)
                     /locus_tag="VFU15_04205"
     CDS             complement(1882..3684)
                     /locus_tag="VFU15_04205"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU4717006.1"
                     /translation="MIKLYRKIIFTVAAAFFFFSGVRSQSMTTAADHLRDTVSLLCAS
                     GDYDSAQSAVLHFLDRKDLLPEDVFTGNFLFADVLKASGKPRDAIAPLLATGKLLDSI
                     PHPERFRSLMYGDVAECYFTLQQYDSAQKYSLLSIDASPDTSFRSGGHAVNDMIIGYN
                     EFLSKKYSVALDYYGRAMLQYTSYGESCELPLVYAKIARVNNAMGERKLAEENISRSI
                     RISDSCGIDDYVLLSERTLFEIYRENGDYKNALSTLEEVNTLVQKIEERKKTAKMNEL
                     KVKYETQLVEKDNERLRNENRKNEEILERQRLALYIALLATVILTFLILLLVRISSQR
                     KKAEAKLAALNSELEEKVKDRTEHLEQARQRIEEDSKKLLFQNKQLIDFCNIISHNLR
                     SPLVNMSMLVDFIEKSNDASEQKQFIEKLKPVIGNLNETFGELLESLQVKQDVEVRSE
                     RINLEDCLKRTLETLEAEVIGSGAVITHDFSAADSIVYPPKYLYSIFQNLVSNAIKYR
                     QPGRKPEIRLLTKREGKSIVLSVKDNGLGIDMEKNGHNLFKIRKVFHQHPDAKGFGLF
                     ITKTQVEAMGGKIRAESEPGNGSVFFVEFTNQDI"
     gene            complement(3962..4747)
                     /locus_tag="VFU15_04210"
     CDS             complement(3962..4747)
                     /locus_tag="VFU15_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019600037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HEU4717007.1"
                     /translation="MQIKIYQVDAFTDKVFGGNPAAICPLDEWLPDELLQGIASENNL
                     AETAFYVKSGQRYEIRWFTPAVEVDLCGHATLAAAYVLFHFEGHAGSEINFYSRRSGN
                     LPVTRNGELLTLNFPADEFVKIPTPDNAVRYFGKFPLEVLKGKTDYMFVFESENDILA
                     LQPDISEIKRLEARGVIVTAKGDRADFVSRFFAPQSGISEDPVTGSAHTTLAPYWAEK
                     LGRNELDAIQLSARRGFLHCANLGRRVEISGMCCAYMTGQIII"
     gene            4853..6196
                     /locus_tag="VFU15_04215"
     CDS             4853..6196
                     /locus_tag="VFU15_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008505589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU4717008.1"
                     /translation="MPALPGRLRFAYAAGMAGWSILINIMGVMLPYFYLSPDNAGWPP
                     LISQTPVLWIINVFALITTAGRLLDAFYDPFIGQLSDRSTNARGRRIPFLKWSFLPSA
                     VFCALVFFPPDRSGTSLNAVWLCVTLTLFFLSSTTYVIPYYALLPELARDPLARVKLA
                     TLQQVGFVCGIVISASTNNIAGLLVDAGLVTDPLESIRLAICLLSAAGAALMILPVFF
                     VDEKKYCESTPAHLPLQKALRETFRNRNFRLFIFSNFLWYMALYIITSGLMYFVTVLA
                     GLPKSLGFLVMGTMVGVSLLYYPFISRWTEKSGKKKIMLAAFFVLAVVCACIFGIGKF
                     PLPEKFQLFAFAAIAAFPLATLGILPPAIVGDIAREEAEKTGENREGFYFAVNYFFVK
                     LGQTLGIAVFSMLTLLGEDPGNDFGIRLSGIVVAVLCALAAFGFSFFREKTRSKV"
     gene            complement(6478..6810)
                     /locus_tag="VFU15_04220"
     CDS             complement(6478..6810)
                     /locus_tag="VFU15_04220"
                     /inference="COORDINATES: protein motif:HMM:NF016330.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HEU4717009.1"
                     /translation="MEKVVLSLENFRNEVDEEIIQRGYIYFRNGWVKTPREVMPGYFE
                     YVVHEVNPHAVSFTHNSDGTFTDIFCTCGDRAHLACRHMAAVLVKMEEEWTESQRTPE
                     WEDLEGKA"
     gene            complement(6903..8108)
                     /gene="pcaF"
                     /locus_tag="VFU15_04225"
     CDS             complement(6903..8108)
                     /gene="pcaF"
                     /locus_tag="VFU15_04225"
                     /EC_number="2.3.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019974020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0019619 - 3,4-dihydroxybenzoate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipyl-CoA thiolase"
                     /protein_id="HEU4717010.1"
                     /translation="MNAAYIIDGVRTPIGNFGGTLSTVRTDDMAALVISGLMKKFPSL
                     DPSLIGDVLLGCANQAGEDNRNVARMALLLAGLPVTVPGETVNRLCASGLSASMAGAR
                     AIMCGDAELIISGGVENMTRGPWVISKASTPFGRDAQMFDSSFGWRFVNPKMKEKFGT
                     DAMGETAENLADRDKISREDQDKFALLSQQKAAKAQERGRFAKEIIPVPIPQKKSDPV
                     LFSKDEFMKPSTTMEGLSKLKPSFRKDGTVTAGNASGLNDGAAALLLASENAIKANNI
                     KPLARIVSMGVAGVEPRIMGIGPVEAGNTALKKAGLKFSDIGVIELNEAFSAQSLACL
                     RSWGIADDDPRVNPNGGAIALGHPLGMSGARILHSAALELQEQNKKYALVTMCIGVGQ
                     GYAVVIEKC"
     gene            8226..9239
                     /locus_tag="VFU15_04230"
     CDS             8226..9239
                     /locus_tag="VFU15_04230"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEU4717011.1"
                     /translation="MKKSTFLISCAGCLCAIALSAQPTLTATGIEPAMSDNLTINNSN
                     YASPGSAGASQTWNLSSLSSTSTATYTGVTPASTPHAASFPSANYAASSSGAYIYYNN
                     TATAWQYYGADNGSIVMPYSNPEDQLHFPFNFNDNYTDNWAATFTNTYTFYRTGSTVV
                     TADGYGTLTTPDGTFGNVMRVHFVQTYQDSTNIASSPYVIYYQNDEYMWYLNGNHYPI
                     AAVYTLTSSAGSPITGGLYLSNVVSGVNETNVLLSSSLAPNPAADHFDLALNLDKSQQ
                     VKVQIFDATGHLVATPVDDAEGIAGENRFGIDVSGLAQGMYFTRVSLNGVPSGTDQLV
                     ITR"
     gene            complement(9310..9735)
                     /gene="paaI"
                     /locus_tag="VFU15_04235"
     CDS             complement(9310..9735)
                     /gene="paaI"
                     /locus_tag="VFU15_04235"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015695535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyphenylacetyl-CoA thioesterase PaaI"
                     /protein_id="HEU4717012.1"
                     /translation="MEANDLAKKVVSKMYDNDWFSQWLGIERVKVEPGNCILKMKVRK
                     EMLNGFGIAHGGITYSLADSALAFASNSHGRMAVSVETSISHTEPLREDDEITATAEE
                     MSLSNKIAIYNVTVKNQKNEIVALFKGTVYRTGKEWFAV"
     gene            complement(9787..11283)
                     /locus_tag="VFU15_04240"
     CDS             complement(9787..11283)
                     /locus_tag="VFU15_04240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717013.1"
                     /translation="MRKAPLAFLLLLPLFLFGSDPKDKGFLRASEGLSTNIATTRKFS
                     VPPASPGKEIIALETPFASADFLEPQLAAMAKGKVIEKIQLVYTTYRESPSFDQHGLN
                     YRRLVNLRKILPDAFTNPLTEWELVPQTGASSPEVGRTYFHGFVIYFRPASSPGLIKD
                     ELHMLDSLFNPAALTGGSSPRDSSGSPHVEYPPSDTAGLKTITLPDGSTVVRDHDIPE
                     DSLRFFLKPSCETCAIVNAKYADSTHKNIIAWEQHGDHRMKRTWKLEEHRGVPTYYTG
                     FADVNLHDPDSVITSTFRRNKWKNMIVVCDITGSMSPYTAQLFSWIPGAVAQGDCAGF
                     VFFNDGDRKSNSQKVVGKTGGIYSTNVMRFDSIYALARKAMQGGDGGDTPENNIEALI
                     YAINKMNPQGDIVLIADNFASPRDLELVDKVNRPVHVILCGARASVNPDYLFIARVTG
                     GSVHTVTQDITNLSQMQEGEEVEIGRQHFLLHDDHFIPIENFSHVNGY"
     gene            complement(11401..12633)
                     /locus_tag="VFU15_04245"
     CDS             complement(11401..12633)
                     /locus_tag="VFU15_04245"
                     /inference="COORDINATES: protein motif:HMM:NF039201.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HEU4717014.1"
                     /translation="MKKAATLFFAGIISFSPLFMQAQMGGWTQRASFPPGGRSAAAGF
                     GLGSAAYISCGIDSAGYKRSTWYYDPAADLWSQLTSLGGITGSGLSRDVAMCFSIGTN
                     AFVVGGQGSIAYMSDTWKYDAVSDTWTQVQSFAGGGRRAGVGFTINGKGYVALGQSST
                     GLKNDTWEYNPGTNVWTQKALYPGTARRLAVAFVINNIAYVGTGDDGVFKGDFYAYDP
                     SANTWSPKAAFGGTPRYGATGFALNNKGYVGFGYDNQLMNRKDFWEYDPVADTWTQMN
                     NFPGTARSNVTAIALPNNHAYMGLGYDSLYREDWWDFDPLSTGISEQTFSANSFTLFP
                     VPMTDHSTLQFDAQAVAQDEKVTIGIYDLAGNEVKEIMSNGNTPVTIGRGNMVPGVYI
                     VSVQPEKSHAVSRRLVVQ"
     gene            complement(12644..13870)
                     /locus_tag="VFU15_04250"
     CDS             complement(12644..13870)
                     /locus_tag="VFU15_04250"
                     /inference="COORDINATES: protein motif:HMM:NF039201.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEU4717015.1"
                     /translation="MRKRYALILPAAVLAVSLSAQNSWEKKTSFGDKRARAVAFSIGT
                     RGYLATGEDTADAEKNDLWEYDPGTDSWTQKASLPATGRRDAVGFSIGNKGYVGTGID
                     ASEAFLGNTLDDFWEYSPQTNAWTQKAPYPGNFGYGIYFATAFTANGKGYVCCGKIGA
                     SNYSQELWEFNPSTNSWTQKAFFPGGVRYGQMSFSLNGKGYVGCGTDENWFTNDFWEY
                     NPVSDTWTQKADFPGSKRSFGTAFSIGQKGYMGLGTDGGYSNDLFEYDPIDNSWAVKA
                     SYGGEGRRSAPSFVLTGAAYVMTGKGTSGKHRDNWEYQPYVTGIDESETVAATVFPDP
                     ASDYLCFSSGNDFSGSGNMTIAVFSADGREVLTAKKAAAPDCRIDVSGLAAGMYSWII
                     TSESGKTANGKFEIQR"
     gene            complement(13867..15057)
                     /locus_tag="VFU15_04255"
     CDS             complement(13867..15057)
                     /locus_tag="VFU15_04255"
                     /inference="COORDINATES: protein motif:HMM:NF024810.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HEU4717016.1"
                     /translation="MKLHLLLIVSVLCVFTAEQTRAQDEYTWTQKTSLPAIGRHRACG
                     CSINNRGYIGLGHINNWNASIIYSDWWEYDPGTDTWMQKADFGGGQRYHAVAFSIGNY
                     AYVGTGSDFGGDHDDLWRYNPVTNTWLMVTQVPGGVRSGAVAFTIDGTGYVALGDYQD
                     DCWAYDPVLNSWGARAVPPVSGYSSVAAVENGKAYVGIGESTGWSEFNPVTNSWTVKA
                     SFPGASRFGSGCFAYNGWVYVVSGSDWSQEFPDSYAYNPLTNTWVQVSDFPGQGRHYF
                     VCFSIGNRAYGGTGTNGTNFNDFWEYGNLAAGTGETEAKNKAVSFFPNPVVDAAVFHF
                     ENALDHEASFSLVDLSGKKVSEEKISPCSDWNFTRGDLAAGNYFYSIVSDGKVISDGK
                     MILQ"
     gene            complement(15069..16265)
                     /locus_tag="VFU15_04260"
     CDS             complement(15069..16265)
                     /locus_tag="VFU15_04260"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HEU4717017.1"
                     /translation="MTKVRTSFFLFLFLAGCLEAQQQYGWIQKAEFGGAARHRVTAES
                     VGNRGYVGLGHINSVVDVLFDDWWEYDPGTDTWTQKANFSGGPRFHCASFVIDNIIYV
                     GTGRDINAVLHQDFWKYDPSSNSWASVASFPGAGRRGAVGFAINGYGYVGTGSGHQNF
                     FRYDPGSDSWLQIASLPGAGRTSAVGFAINGKGYVGTGDIGGPSGDMWEYDPASDTWT
                     AKANLPGLPRMEAGGFVLDGKGYLGTGDDYSSGTNYQDFWCFDPQMNSWTQVADFAGA
                     ARRYLSCFTIGNRAYAGLGTSGINYADFWEYGSISGTEEFTQDKTVSVFPDPADQSAE
                     ISVENAEGEVSVLVLASDGKCVARQPVVSGKASLDCSGLAPGIYFLQFSVSGKIFSNR
                     KLVVRH"
     gene            complement(16273..16662)
                     /locus_tag="VFU15_04265"
     CDS             complement(16273..16662)
                     /locus_tag="VFU15_04265"
                     /inference="COORDINATES: protein motif:HMM:NF027575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4907 domain-containing protein"
                     /protein_id="HEU4717018.1"
                     /translation="MKRLRVAASLLLLPLFFVGCDAAAPGSPPASIPATTDTARHDAP
                     VHVEAKTFAVTDSMGAQAGWGYDLYVNGKKTIHQPIIPGVPGNNAFPSEEQARKTGEY
                     AASKMERSGTFPSLSVKELDSLGITHN"
     gene            complement(16749..17372)
                     /locus_tag="VFU15_04270"
     CDS             complement(16749..17372)
                     /locus_tag="VFU15_04270"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009087563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU4717019.1"
                     /translation="MGTSPKEHWEKVFATKAENEVSWFQPYPKTSVEFLELFDLPLHA
                     NIIDVGGGDSHFIDVLVERGYRNIYLLDISSNAIERTKKRLGSKADHVHYIVSDITEF
                     TPPVQFDFWHDRAAFHFLTDEERINRYVSIAEKGISEKGYLILGTFSEAGPEKCSGLD
                     IRQYSQASMSSRFEKRFERIRCVTEDHVTPFNTTQNFLFCSFRKRAE"
     gene            complement(17388..17750)
                     /locus_tag="VFU15_04275"
     CDS             complement(17388..17750)
                     /locus_tag="VFU15_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015812575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-associated domain-containing
                     protein"
                     /protein_id="HEU4717020.1"
                     /translation="MKKFISNFLLLLVFLSVNALKAQDTAVVKNSDGTATVKIKTSAI
                     CDQCKDRIEGQVNLMKGVKKATLDTATKVLTVNYDPGKTSPDKIRSVISHTGYDADDV
                     KADPNAKSRLPGCCRKPE"
BASE COUNT         4057 a         4974 c         4543 g         4334 t
ORIGIN      
        1 gacgaagcca tgaagatcgc agtgacgatc tcgcagatgc cgaccaaagc gatcgggctg
       61 acgaaacgtt tgctcaatga aggaacggtg aattcgcttg atgagcagct ggtgcgcgaa
      121 ggcgaagagc aggtggcagc cacgcgcaca tacgatttta ccgaaggcgt gaaagcgttt
      181 cttgaaaaac ggaaaccgga attcagggga gaataatttt tatttctcat tttgcatgcc
      241 gggcgccttg cccccggagc acaacagtta aaattcgttt atatggatca acatacggtc
      301 gtcggcgtca tcggttccgg cgccatggga agcgggatcg cgctcgtggc agctactgcc
      361 ggtcacaagg tctttcttta tgacaacaac gcgaaacaac tggcaaaagc cgaagaaacg
      421 cttctgcagc tgctgaagag aatcgttgaa aaaggaaagc tccggcagga agatgcggac
      481 acgatcttta aacgtcttgt tttttccgcg gagatgtctt ccttcagcga atgcgggctc
      541 gtgatcgaag ccatcgtgga aaacatcgat gtgaagcaaa aggttttttc agacctggaa
      601 aacattgtcg gcgaaagctg cgtgctcgct tcaaacacct cttcgctttc gatcacctcc
      661 atcgcttcgg cgtgtaaaaa aacaggtcgc gtgatcggga tccacttttt caatcctgcg
      721 ccgctcatgc cgctggcaga gataattccc ggcatttcga cggaccggaa tattcttgcc
      781 gcgtgcaaaa cgctcatcag cggatgggga aaaagcgtcg tgcaggcaaa agacacaccg
      841 ggcttcatcg tcaaccgggt ggcgcgttcc ttctacggcg aatcgatacg catttacgaa
      901 gaaggcatcg cggatttcgc cacgatcgac tgggcaatga aaacgtacgg cggttttaaa
      961 atgggtccgt tcgagctgat ggacttcatc ggcaacgacg tgaattataa agtgacggag
     1021 accgtctggg aacaattctt ttacgagccg cgcttcaaac cttcgctgac gcagaaacgg
     1081 ctgtacgaag gaaaaatgtt cgggcggaaa tccgggcaag gctattacga ttaccgggaa
     1141 ggcgccgtga tgcccgaacc tgtaaaagac gaaacactgg gaaaagccat tttcttccgc
     1201 gtgctcgtga tgctcatcaa cgaagcggcg gattcacttt acctcggtct tgctacgcgc
     1261 gaggacatcg acactgcaat gacgaaaggt gtgaattatc ccaaaggact gctgcgctgg
     1321 gccgatgaaa ccggcattgc cgctgttacc ggaaagctgt catcacttta cgaagaatac
     1381 ggggaagacc gttaccgtcc gtgtgtgctg ctcaaacgga tgaccaaaga aggcaaaaca
     1441 ttttactgag aacaggaacg cgctttcaat tattcccttc ctgtcgtgtg agccgttccc
     1501 tgatcaggcc gatcgtttcc gcggtaacag gcttgacgat aaaatccgaa acatagctga
     1561 agcttttcgc acgttccact tcgtgcggcg aaacggaaga ggacacgacg tagaggtcga
     1621 tctttcccgg gagcttcttc cgcagcgttt cgtattcggc caggaatccc cacccgtcga
     1681 gtaccggcat gttcaggtcg aggagaatga catccggcag cttctccggt tccggcagca
     1741 atttttccag gaaacggatc gcatccattc cgttggcgca cacattcact tcaccgacga
     1801 gccgcgattc gtcaattgtt tttttcgtaa taaaaatgaa aacatcatca tcatcaacga
     1861 gcagcaattt cgtttccttc ttcatatatc ctggttggtg aactccacaa aaaaaacact
     1921 tccgttaccg ggctcgcttt ccgcccggat ctttccgccc atcgcttcca cctgtgtttt
     1981 ggtaatgaaa agcccgaatc ctttcgcgtc cggatgctgg tggaacacct tgcggatctt
     2041 gaacaggtta tgcccgttct tttccatatc gatgccgagg ccgttatcct tcaccgaaag
     2101 aacaatgctc tttccttccc ttttcgtcag cagccgtatt tcgggtttcc ttcccggctg
     2161 gcggtatttg atcgcgttgc tcaccaggtt ctgaaaaatg ctatagagat atttgggcgg
     2221 gtaaacgatg gaatccgcag cagagaaatc atgcgtgata acggcacccg acccgatcac
     2281 ttccgcttcg agcgtttcca gcgtgcgttt caggcaatct tcgaggttta tccgttccga
     2341 tctcacctcc acatcctgct tcacctgcaa tgactcgagc agttcgccga acgtttcgtt
     2401 caggttgccg atcacgggtt tcagcttttc aatgaactgt ttctgttccg acgcatcatt
     2461 gctcttttcg ataaaatcca cgagcatcga catgttcacc agcggcgacc gcaggttatg
     2521 cgaaataata ttgcagaaat cgatgagctg cttgttctgg aacaaaagtt ttttcgaatc
     2581 ctcctcgatc ctttgcctgg cctgttccag gtgctctgtc cggtctttca ccttctcttc
     2641 cagctctgaa ttgagcgcgg ccagcttcgc ctcggccttt ttccgctggc tggagatgcg
     2701 gaccagcagg aggataagaa aagtaagaat gaccgtggcg agcagtgcaa tgtacagggc
     2761 gagacgctgg cgctcgagga tctcttcgtt cttccggttc tcgttccgca ggcgctcatt
     2821 gtctttctcc accagctgcg tttcatattt cactttcagc tcgttcatct tcgccgtttt
     2881 cttccgctct tcgatcttct gcaccagcgt attcacttcc tcgagcgtgg aaagcgcgtt
     2941 cttataatcg ccgttctcgc ggtagatctc gaacagcgtg cgttcggaaa gcagcacata
     3001 atcgtcaatg ccgcacgaat cgctgatccg tatcgagcgg ctgatgtttt cttccgccag
     3061 cttcctctct cccatcgcgt tgttcacgcg cgcgatcttc gcatagacga gcgggagctc
     3121 gcaggattct ccatagctgg tgtattggag catcgccctt ccgtaataat ccagggcgac
     3181 ggaatatttt ttcgagagga attcgttgta gccgatgatc atgtcgttca ccgcatggcc
     3241 gcccgaacgg aagctcgtat ccgggctggc gtcgatggac agcagcgaat atttctgcgc
     3301 actgtcgtat tgctgcaacg tgaaataaca ttccgccaca tcgccgtaca tcagggaacg
     3361 gaatctctcc gggtgaggaa tactgtccag cagtttgccc gttgcaagca aaggcgcgat
     3421 cgcatcccgc ggtttcccgg aagctttgag tacatccgca aaaagaaaat taccggtaaa
     3481 gacatcttcg ggaagaagat ctttcctgtc gagaaaatgc agcactgccg actgggccga
     3541 atcataatca cccgaagcgc aaagcaggga gaccgtatca cgcaaatgat cagccgcggt
     3601 ggtcatcgac tggctgcgca ctcctgaaaa aaagaagaaa gcagcggcaa cggtgaaaat
     3661 tattttcctg tacagcttta tcatgcgttg ttaccgcttc atccgggttt aagcatttgc
     3721 catacagggt acaaaactca aggcgcctgc acagatttta ttaaatatac caaggcttcg
     3781 gcgaattttc aacgttcccg tttttttgcc ggcacattat tcccggctgg ccgcattttc
     3841 ttctcagaaa ccgatttagt tatcattcgc ccgtgaacgg tctgctcctt ttaccctgtc
     3901 accggttttg tttttcgttt accagcgcag caagacgcac caaggttttg ggaaacgatc
     3961 ttcagatgat gatctgcccc gtcatgtacg cgcagcacat cccgctgatc tctacacgtc
     4021 ggccgagatt tgcgcagtgc aggaaacctc tccgcgcgga aagctgtatc gcatcgagct
     4081 cattcctgcc gagcttttcc gcccagtaag gtgcaagcgt agtgtgcgcg gaaccggtca
     4141 cggggtcttc gctgatcccc gactgcggtg cgaagaaacg ggaaacaaaa tccgcgcgat
     4201 ctcctttggc cgtcacgata acgccgcggg cctcgagccg ttttatctcg ctgatgtccg
     4261 gctgcagcgc aagaatatca ttctcgcttt cgaagacgaa catataatcc gttttccctt
     4321 tcagcacttc gagcgggaat tttccgaaat aacggaccgc gttatcgggt gtgggaattt
     4381 tgacgaactc atcggccggg aaattcagcg tgagcagttc gccgttgcgg gtgaccggca
     4441 gattgccgct tcgccgcgaa tagaaattta tttccgatcc tgcatgtcct tcgaaatgaa
     4501 aaagcacgta cgccgccgcg agcgtggcgt gaccgcagag atcgacttcg acggccgggg
     4561 tgaaccagcg tatctcgtac cgctgcccgc ttttcacgta aaacgcggtt tcagcgaggt
     4621 tgttttccga ggcaattccc tgcagcagct catcgggaag ccattcgtcg agcgggcaaa
     4681 tcgctgccgg gttgccgccg aaaactttgt cggtgaatgc atccacctgg taaatcttta
     4741 tctgcatcgc gttcgaataa aaattccggg aaaatccact ccgaaattta cgcaaaaacg
     4801 aagcgttgcg cattaggatt ttctcatctg ccgatttcat ttacctttaa acgtgcccgc
     4861 tcttcccgga agattacgtt ttgcctatgc cgccggaatg gcaggatgga gcatcctgat
     4921 caacatcatg ggcgtgatgc tgccgtattt ctacctgtcg cccgacaatg caggatggcc
     4981 gccgctgata tcgcagacac ccgtgctgtg gatcatcaac gtattcgctc tcatcacgac
     5041 ggcaggccgc ctgctcgatg cattctacga tccgttcatc ggccagctca gcgaccgcag
     5101 cacgaacgca cgcgggcgaa gaattccttt cctgaagtgg agcttcctgc cttccgcggt
     5161 tttctgcgcg cttgtttttt ttccgccgga ccgcagcggc acttccctca atgccgtgtg
     5221 gctgtgcgta acgctgacgc tgttcttcct ttcatccacc acctatgtca ttccttatta
     5281 cgcgctgctt ccggagctcg cacgtgaccc gcttgcgcgc gtaaagctgg ccacgctcca
     5341 gcaggtaggt ttcgtatgcg gcatcgtcat ttccgcttcg acgaacaaca tcgccgggct
     5401 gctggtggat gcgggacttg tcaccgaccc gctggaaagc atccgcctcg cgatctgcct
     5461 gctgagcgct gcaggcgctg cgctgatgat cctgcctgtt tttttcgtgg atgaaaaaaa
     5521 atactgcgaa agcacacctg cgcaccttcc gctgcagaaa gcattgcgcg aaactttccg
     5581 gaaccggaat ttccgcctgt tcatcttttc gaattttctc tggtacatgg cgctgtacat
     5641 tatcacgagc gggctgatgt atttcgtgac cgtgctcgcc ggacttccga aatcactcgg
     5701 gttcctcgtg atgggaacga tggtgggcgt ttcgctgctg tactacccgt tcatcagccg
     5761 gtggacggaa aagagcggca agaaaaagat catgctcgct gcatttttcg tgctggccgt
     5821 ggtgtgcgcc tgcattttcg ggatcgggaa atttccgctg cccgaaaaat tccagctgtt
     5881 cgctttcgcc gcgatcgctg cgttcccgct tgccacgctg ggtatccttc cacccgccat
     5941 cgtcggggat attgcgcgtg aagaagcgga aaagacaggc gaaaaccggg aaggatttta
     6001 tttcgcggtc aattattttt tcgtgaagct ggggcagacc ctgggcattg ccgttttttc
     6061 catgctgacg ctgctgggtg aagatccggg aaatgatttc gggatacgct tgtcggggat
     6121 cgtcgtcgcg gtgttgtgtg cgcttgcggc gttcggcttt tcgtttttca gggagaaaac
     6181 caggtccaaa gtttaaagtt ccggtttcac gttcggaata tgcccaggcc tggataacgt
     6241 tgaccgtttt cagttaccag tttgccgttt caactggttt gagtcactaa ctcttctttg
     6301 tgcgctttgt gcagaactcc gtgtgctttg tgtttgaaga gatctgctgg gcgtttgaac
     6361 tttgattttt ggaattgcta tccgtttgtt atttccaaac tccgaacgcg aagctgaaaa
     6421 ccgccggcag aaaactattt aattcctcaa aaatttcagg caaaaaaacc cgctgcgtta
     6481 cgctttccct tcgagatctt cccattccgg cgtacgctgc gattcggtcc actcctcttc
     6541 catcttaacc agcacggcag ccatgtggcg gcaggcaaga tgcgcccggt cgccgcaggt
     6601 acagaaaatg tccgtgaacg tcccgtcgct gttgtgcgtg aatgaaactg cgtgcggatt
     6661 cacttcatgc acgacgtact cgaaataacc gggcattact tcacgcggcg ttttcaccca
     6721 cccgttacgg aaataaatat acccgcgctg gatgatttct tcatccacct cgttccggaa
     6781 attctccaat gaaaggacga ctttttccac ggtgcaaaaa taagaacaac tttacggaat
     6841 cacatcccgg ttaaaccgaa aatagaatgg aaaacagaaa gagataaaag acggacgcgg
     6901 cttcagcact tttcgatcac caccgcatat ccctgcccta cgccgatgca catcgtaacc
     6961 agcgcatatt ttttattctg ctcctgcagc tcgagcgcgg cggaatgaag aatacgcgcg
     7021 ccgctcatgc ccagcggatg cccgagcgcg atcgcgccgc cgttcgggtt cacgcgtgga
     7081 tcgtcatctg caattcccca tgagcgcagg cacgccagcg attgtgcaga gaacgcttcg
     7141 ttcagctcga tcacaccgat atcggaaaat ttcagtcctg cttttttcag cgccgtgttg
     7201 cccgcttcca ccggcccgat gcccatgatg cgcggttcca ccccggccac gcccatggaa
     7261 acgatgcgcg ccagcggttt gatgttgttt gcttttatcg cattttccga agcgagaagc
     7321 aatgccgcag cgccgtcatt gagcccggac gcgttgcccg ctgtaaccgt tccgtctttg
     7381 cggaaagaag gcttcagctt cgaaagtcct tccatcgtgg tggacggttt catgaattca
     7441 tcttttgaaa aaagtacggg atcgcttttt ttctgcggga tgggaacagg aatgatctct
     7501 ttcgcgaaac gtccccgttc ctgcgctttc gccgctttct gctgtgaaag cagtgcgaat
     7561 ttatcctggt cctcacgtga aattttatcg cggtcggcca ggttttctgc cgtttcaccc
     7621 attgcgtccg ttccgaactt ctctttcatt ttcgggttca cgaaacgcca gccgaagctc
     7681 gagtcgaaca tctgcgcatc gcgcccgaaa ggtgttgacg ctttcgagat cacccacggt
     7741 ccgcgcgtca tgttctccac cccacctgaa atgatcagct ccgcatcgcc gcacatgatc
     7801 gcacgcgcgc ccgccatgga agcggaaagt cccgaagcgc agagacgatt cacagtttca
     7861 cctggcaccg tgacgggaag tcctgcaagc agcaatgcca tacgtgcgac gttgcggtta
     7921 tcctcgcccg cctggttggc gcagccgagg agcacatcgc cgataagcga aggatcgagg
     7981 gaaggaaatt ttttcatgag cccgctgatg accagcgcgg ccatgtcgtc ggtacggaca
     8041 gtggaaagcg ttccgccgaa attcccgatg ggagtgcgga cgccgtcgat gatataagct
     8101 gcgttcataa ggaagatatg aggacgcaaa attaccgaaa agaaaattgg tgctgtccac
     8161 aaaaaactct tgcgtcagca gtattttgta tatttaataa acaacaaatc tcaactccga
     8221 agttcatgaa aaaatctact ttccttatca gctgtgcggg atgtctctgc gccatcgcgc
     8281 tctctgcaca acctacgctg accgccaccg ggatcgaacc cgcgatgagc gacaatctta
     8341 cgatcaacaa cagcaactac gcaagcccgg ggagcgcagg cgccagccag acatggaacc
     8401 tttcctcgct gagctctacc agcaccgcaa cgtacacggg cgtgactccc gcatcgacac
     8461 cgcacgcagc gagctttccc tccgccaact atgcggccag ttccagcgga gcctacatct
     8521 attataacaa tacggccacc gcatggcagt attatggcgc cgataacggc agcattgtca
     8581 tgccttattc caaccctgaa gaccagcttc actttccgtt caacttcaac gacaactata
     8641 ccgacaactg ggctgcaacg tttacgaata cgtacacctt ctaccgcacg ggatcgacgg
     8701 ttgtcacagc cgacggctat ggaacgctga cgacgccgga cggaactttc gggaacgtga
     8761 tgcgtgtaca tttcgtgcag acgtaccagg attccacgaa catcgcgagc tcgccgtacg
     8821 tcatctacta ccagaacgac gaatacatgt ggtacctcaa tggcaaccac tatccgatcg
     8881 ctgcagtgta cacactcaca tcgagcgccg gttccccgat caccggcgga ctttacctga
     8941 gcaacgtcgt gagcggcgtg aacgaaacga atgtgctgct ctcttcatcg ctcgcaccga
     9001 acccggctgc ggatcatttc gatctcgccc tgaacctcga taagtcgcag caggtaaaag
     9061 tgcagatctt cgatgcgacg ggtcacctcg tagctacacc ggtggacgat gccgaaggaa
     9121 ttgcaggaga gaaccgtttc gggatcgatg tttccgggct tgcgcagggc atgtatttca
     9181 cccgcgtctc gctgaacggt gtaccttccg gcacagatca gctggttatc acccggtgaa
     9241 cgcaacgtac tttttaaaac aaaaaggatc tccgcggaga tcctttttta ttgttgcagg
     9301 ttttcgtagt taaaccgcga accattcttt gcctgtgcgg taaaccgttc ctttaaaaag
     9361 cgcgactatt tcgttctttt gatttttcac cgtcacatta tagatcgcga tcttgtttga
     9421 aagagacatt tcttcggctg tcgcagtgat ctcgtcatct tcgcgcagcg gttccgtgtg
     9481 cgagatggac gtttccacgg acaccgccat tctcccgtgg ctgttcgacg caaaagcaag
     9541 cgcgctgtcg gccagggaat aggtgattcc gccgtgcgca atgccgaaac cgttcagcat
     9601 ttccttccgt actttcattt tcaggatgca gttcccgggt tccactttta cgcgttcgat
     9661 tccgagccac tggctgaacc agtcgttgtc gtacattttc gagacgactt tttttgcaag
     9721 atcgtttgct tccatgaatt aaaaatacgt gctaaaacct catcgccggc atgaaataac
     9781 ggagattcaa tacccgttca catgcgagaa attctcgatc gggataaaat gatcatcgtg
     9841 cagcaggaaa tgctggcggc cgatctccac ttcttctcct tcctgcatct gtgaaaggtt
     9901 cgtgatgtcc tgcgtcaccg tgtgcactga tccgcccgtg acgcgcgcga tgaagagata
     9961 atcgggattg accgaagcgc gcgccccgca aaggatgaca tgcacaggcc ggttcacttt
    10021 gtcgaccagc tcgagatcgc ggggtgaagc gaaattgtcc gcgatgagaa cgatatcgcc
    10081 ctgcgggttc attttgttga tcgcgtaaat gagcgcttca atgttgttct cgggcgtgtc
    10141 gcccccgtcg ccgccctgca tggctttccg cgcgagcgcg tagatgctgt cgaaacgcat
    10201 cacgttcgtg ctgtagatgc cgcctgtttt cccgaccact ttctggctgt tcgatttgcg
    10261 atcgccgtcg ttgaaaaaaa caaaacccgc acaatcgccc tgcgccaccg cgccgggtat
    10321 ccacgaaaaa agctgcgccg tatacggcga catgctgccc gtgatatcgc acaccacgat
    10381 catgttcttc cacttgttgc gccggaatgt agacgtgatc accgaatcgg gatcgtgcag
    10441 gttgacgtcc gcaaagccgg tataatacgt aggaacaccg cggtgctctt ccagcttcca
    10501 cgtccgcttc atccggtggt cgccgtgctg ttcccacgca ataatattct tgtgtgtcga
    10561 gtccgcatat tttgcattga cgatcgcgca ggtctcgcag gaaggtttca gaaaaaaacg
    10621 caggctgtct tccgggatat cgtgatcgcg tacaacggtg gagccgtcgg gaagcgtgat
    10681 cgttttgagc cctgccgtgt cggaaggcgg atattccaca tgcggagaac cggagctgtc
    10741 gcgcggggaa gatccgccgg tgagcgctgc cggattgaaa agagaatcga gcatgtgcag
    10801 ctcatccttt atcaggccag gtgaagaagc aggacggaaa taaatcacga agccgtgaaa
    10861 gtaggtgcgg cctacttccg gggaggacgc gccggtttgc ggaacgagct cccattccgt
    10921 cagcgggttt gtaaaagcgt cgggaagaat tttccggaga ttcaccagcc gccggtaatt
    10981 cagtccgtgc tgatcgaacg aaggtgattc gcggtacgtc gtgtatacga gctgtatttt
    11041 ctcgatcact tttccttttg ccattgcggc cagctgcggt tccaggaaat cagcggaagc
    11101 aaaaggcgtt tcaagagcga taatttcttt tcccggcgaa gcaggcggta cggaaaattt
    11161 ccgtgtcgtc gcaatattgg tggaaagacc ttccgaagca cgcaggaacc ctttgtcttt
    11221 aggatcggac ccgaaaagaa acagcggcag gagaagtaaa aaagcaagcg gggcttttcg
    11281 catagaccgg caggaaccat tactataatt ctcctgcaaa aaaaagatac acgaaagtaa
    11341 aacgaaaaac ggtggagatg cttcaccgtt ttttaaaaaa gaaaattttt ccggctgcgt
    11401 ctattgcaca acgagccgcc gggaaaccgc gtgcgatttt tccggctgca cgctgacgat
    11461 gtaaaccccg ggcaccatgt tcccgcgacc gatcgtcacc ggcgtgttcc cgttcgacat
    11521 gatctctttc acttcatttc ccgcgagatc ataaataccg atcgtcactt tttcgtcctg
    11581 cgcgacagcc tgcgcatcga actgcagtgt ggaatgatct gtcatcggga cgggaaacag
    11641 cgtgaatgaa tttgcagaga atgtttgttc gctgatgccg gtcgaaagcg gatcgaaatc
    11701 ccaccagtct tcccggtaca ggctgtcata cccgagaccc atgtacgcgt ggttgttcgg
    11761 aagggcgatg gccgtcacat tcgagcgcgc cgttcccgga aaattattca tctgcgtcca
    11821 cgtatcggcg acgggatcgt attcccagaa atctttacgg ttcatcagct ggttgtcata
    11881 accaaaaccg acgtatcctt tattgttcag cgcaaaaccg gtcgcaccgt aacggggcgt
    11941 tccgccgaat gcagctttgg gcgaccacgt attcgcagaa ggatcgtagg cataaaaatc
    12001 tcctttgaaa acaccgtcgt caccggtccc gacataagcg atgttattaa tgacgaaagc
    12061 gacagcaagc ctgcgcgccg tgcccggata cagtgctttc tgcgtccaga catttgtacc
    12121 ggggttatat tcccaggtgt catttttcaa tcctgtggaa gactgcccga gcgccacgta
    12181 tcctttcccg ttgatggtga atcccacgcc ggccctgcgg cctccgccgg caaagctctg
    12241 cacctgcgtc cacgtgtcgc tcaccgcatc gtatttccat gtgtcgctca tgtacgcgat
    12301 gcttccctgt cccccgacaa caaatgcatt cgttccgatc gagaagcaca ttgcaacgtc
    12361 gcggctgagt ccgctgccgg tgataccgcc gagacttgtc agctgcgacc agagatccgc
    12421 cgcgggatca taataccagg tgcttcgctt gtatcctgcg ctgtcgatgc cacacgaaat
    12481 gtaagcggca gagccgagac caaatccggc agcggcggaa cgaccgccgg gcgggaacga
    12541 agcgcgttgt gtccatcctc ccatctgcgc ctgcatgaag agcgggctga aagaaattat
    12601 tcctgcaaaa aacagagtag ctgccttttt cataaggggc tttttatcgt tgaatttcaa
    12661 attttccatt ggcggttttt ccggattcag atgtgatgat ccaggaatac attcctgcag
    12721 ccaggccgga aacatcaatg cggcaatccg gcgcagccgc tttttttgcc gtgagaactt
    12781 ctcttccgtc cgccgaaaaa accgcgatcg tcatatttcc ggaaccgctg aaatcattgc
    12841 cggatgaaaa gcagagataa tccgaagccg ggtcaggaaa aacagttgcc gcaaccgttt
    12901 cgctctcatc aatgcccgtc acataaggct ggtattccca gttgtcacgg tgctttccgc
    12961 tcgtgccttt tcccgtcatc acgtaagccg cgccggtgag aacgaaggaa ggagcggaac
    13021 gtcttccttc accgccatag gacgctttta ccgcccacga attatcgatc ggatcgtatt
    13081 cgaaaagatc gttgctgtag ccgccgtccg ttccgagtcc catatatcct ttctgcccga
    13141 tggaaaatgc cgttccgaac gaacgtttcg atccgggaaa atccgctttc tgcgtccatg
    13201 tatccgatac cgggttgtat tcccagaaat cattggtgaa ccagttctcg tccgttccgc
    13261 agccgacgta tccttttccg ttcagcgaaa aactcatctg cccgtaacgg acaccgccgg
    13321 ggaaaaaagc tttctgcgtc caggaattgg tggaaggatt gaattcccac aactcctgcg
    13381 aataattgga agcgccgatc tttccgcagc acacatatcc ttttccgttc gcagtgaatg
    13441 ctgtcgcaaa ataaattccg tagccgaagt tgccgggata cggcgctttc tgcgtccatg
    13501 cattggtttg cggagaatat tcccagaaat cgtccagcgt atttccgaga aaggcttccg
    13561 atgcatcgat ccccgtgccg acatatcctt tgttgccgat cgaaaaaccg acagcatcgc
    13621 gacgcccggt cgccggaaga ctggctttct gcgtccacga atccgttccg ggatcatatt
    13681 cccacagatc gtttttttct gcgtcggccg tgtcttcgcc cgttgcgaga tagccgcgcg
    13741 tgcctatgct gaacgcaacc gcgcgcgctc tcttgtcacc gaaagacgtt ttcttttccc
    13801 agctgttctg agcggaaaga gatacggcga ggacggcggc gggaagaatg agtgcgtatc
    13861 tttttctcat tgcaggatca ttttaccatc tgaaataact tttccatccg aaacgatgct
    13921 gtagaaataa tttccggcgg caagatcacc gcgcgtgaaa ttccagtcgc tgcagggaga
    13981 tattttttct tcacttactt ttttaccgga aagatcgacc agtgagaacg aggcttcgtg
    14041 atcaagcgcg ttctcaaaat gaaatacggc agcgtcgaca acggggttcg ggaaaaatga
    14101 aaccgcttta ttcttcgctt ccgtttcacc tgtgcccgcc gcgaggtttc cgtattccca
    14161 gaaatcattg aaattcgttc cgttcgtacc tgttcctccg tatgcgcggt taccgatgct
    14221 gaagcagacg aaataatgcc ggccctgccc cgggaaatcc gaaacctgta cccaggtatt
    14281 cgtcagcggg ttgtaagcat acgaatccgg gaactcctgc gaccagtcgc tgccggaaac
    14341 cacgtacacc catccgttgt aagcaaaaca cccggaaccg aaacgcgacg caccagggaa
    14401 ggaagctttg acggtccagc tgttcgtgac cgggttgaac tcagaccatc ccgtgctttc
    14461 cccgatgccg acgtacgctt ttccattctc cacagcagca accgaagaat acccgcttac
    14521 cggtggaacc gctcttgctc cccaggaatt cagaaccggg tcatacgccc agcaatcgtc
    14581 ctggtaatca cccagcgcta cgtaaccggt gccgtcgatc gtaaaagcga ccgcgcccga
    14641 acgcacacca cccggaacct gcgtcaccat cagccacgtg ttggtaacgg gattgtatcg
    14701 ccacaggtcg tcgtgatctc cgccgaaatc cgatcctgtt cccacatacg cataattccc
    14761 gatgctgaat gcgacggcat gatagcgctg cccgcctccg aaatccgctt tctgcatcca
    14821 ggtgtccgtg ccgggatcgt attcccacca gtcgctgtag atgatcgagg cgttccagtt
    14881 gttgatgtga ccgagcccga tgtacccgcg gttattgatc gagcatccgc aggcgcggtg
    14941 ccgcccgatc gcgggcagcg acgttttctg cgtccacgtg tattcatcct gcgcacgtgt
    15001 ttgttcagca gtgaaaacac agagcacgga aacgatcagc agcagatgta atttcatgat
    15061 gctttttttt aatgcctgac aaccagtttc cggttggaaa aaatcttccc gctgacggaa
    15121 aactgcagga aataaattcc gggggcaaga ccgctgcaat ccagtgatgc tttcccggaa
    15181 acaaccggct gccgggccac gcattttcca tcggaagcaa gaacgagaac ggaaacttct
    15241 ccttcggcgt tttccacgct gatctctgcc gactgatcgg caggatcagg aaataccgaa
    15301 acggttttat cctgtgtgaa ttcttccgtt ccgctgatgg agccgtattc ccagaaatcc
    15361 gcatagttga tgccgctcgt tcccagcccg gcatacgccc tgttcccgat ggtgaagcag
    15421 gacaaatacc ttcttgccgc tccggcgaaa tcagcgacct gcgtccacga attcatttgc
    15481 ggatcgaagc accagaaatc ctggtaattc gttccgctcg aataatcatc accggtaccg
    15541 aggtatcctt tcccgtcgag cacgaatccg cccgcttcca tacgcggaag gccgggaaga
    15601 tttgcttttg cggtccacgt atcgcttgcc ggatcgtatt cccacatgtc cccggaaggt
    15661 ccgccgatgt cgcccgtgcc tacatatcct tttccgttta tggcaaaccc gacggccgat
    15721 gttctgcctg cgcccggaag ggaagcgatc tgcagccacg aatcactgcc cgggtcatag
    15781 cggaaaaaat tctggtgacc ggaaccggtt ccgacataac cgtatccgtt gatggcaaaa
    15841 ccgaccgctc ctcttctccc tgcgccggga aacgaagcga cagaagccca tgaattggaa
    15901 gacgggtcgt atttccagaa atcctggtga agcacggcgt ttatatcacg gccggttccc
    15961 acgtaaatga tattatcaat gacgaagctg gcgcagtgaa aacgcggtcc gccggaaaag
    16021 ttcgccttct gcgtccatgt gtctgttccc ggatcgtatt cccaccagtc gtcgaacaga
    16081 acatccacga cggaattgat atgcccgagt cccacgtacc cgcggttgcc cacggattct
    16141 gcagttacac gatgacgcgc agcgccgccg aactcagctt tctgtatcca cccgtattgc
    16201 tgctgcgcct ccaggcaccc ggcaaggaag agaaaaagga aaaagctggt gcgcaccttt
    16261 gtcattcgcg ggtcagttat gcgttatgcc gaggctgtcc agctccttta cgctaagcga
    16321 agggaatgtc ccgctgcgtt ccattttcga ggcagcgtat tctcccgttt tcctcgcctg
    16381 ttcttcggaa ggaaaagcat tattcccggg aacacccgga atgatcggct ggtgaattgt
    16441 tttctttccg tttacataaa gatcatatcc ccatcccgcc tgcgcgccca tgctgtcggt
    16501 cacggcaaac gtttttgcct caacatgcac cggggcgtca tgtcgcgccg tatcggtggt
    16561 tgccgggatg gaagccgggg gagaaccggg agctgcagca tcgcagccaa caaaaaaaag
    16621 gggaagcaga agtaaggaag cagcaacccg taagcgtttc atggaggaac ctttctcttt
    16681 tacaacgata caaaaattta acgttaaata aaagttcggt tcacatcccg aaatccgccc
    16741 cccgactgct attccgcccg tttccggaag ctgcagaaga gaaaattctg cgtggtgttg
    16801 aagggtgtaa cgtgatcctc cgtcacgcac cggatgcgtt caaaacgttt ttcaaaacgc
    16861 gacgacatcg aggcctgcga atactgccgt atgtcgaggc cgctgcattt ttccgggccc
    16921 gcttctgaaa aagtgccgag aatgagatac cccttttcgc ttatgccttt ttccgcaatc
    16981 gaaacgtaac ggttgatgcg ctcttcgtcc gtcaggaaat ggaatgctgc acggtcgtgc
    17041 cagaaatcga actgtaccgg cggtgtaaat tcagtgatgt cggacacgat gtaatgcaca
    17101 tggtctgctt tactgccgag acgttttttc gtccgttcga tcgcgttgga ggaaatatcg
    17161 agaagataaa tattccggta gccacgctcc accagcacat cgataaaatg gctgtcgcct
    17221 cctcccacgt cgataatatt cgcgtggaga ggaaggtcga acagctcgag gaattcaacg
    17281 gaggttttgg gatagggctg gaaccagctc acttcgtttt ccgccttcgt ggcaaaaact
    17341 ttttcccagt gctcttttgg cgatgttccc attttgtgct tcctgtgtta ttccggtttc
    17401 ctgcagcatc ccggcaatct tgatttcgcg ttcggatcgg ctttcacgtc gtccgcatca
    17461 tacccggtat gcgaaattac actcctgatc ttgtccggcg atgtttttcc cggatcataa
    17521 ttcaccgtca gcactttcgt cgcggtgtcg agcgtggctt tcttcacacc tttcataaga
    17581 ttcacctgtc cctcgatgcg gtccttgcat tgatcgcaga tcgccgaggt tttgatcttc
    17641 accgtcgctg ttccgtcgct gttcttcaca acggcggtat cctgcgcttt cagcgcattc
    17701 acggaaagaa aaacaaggag aagaaggaaa tttgaaatga actttttcat gactgtttgt
    17761 tttaaataga ggattgtttt ttattcgatt ctcaggcgta atccgccgta aataattctt
    17821 ccgtccgtag gcgcatagat aagcgaggcg tcaaaatgcg gaccaaacgg atcagcagca
    17881 gcaatgacag gatccttctg ccggaaat
//
LOCUS       DASTSF010000145         4834 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1578793,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000145 DASTSF010000000
VERSION     DASTSF010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4834
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1578793"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="VFU15_04280"
     CDS             complement(<1..180)
                     /locus_tag="VFU15_04280"
                     /inference="COORDINATES: protein motif:HMM:NF019749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEU4717021.1"
                     /translation="MKKIILAAGALFVLASLNAQTVVYHENFEVADSVTATGTPLWGP
                     DLTVQTNYLTSYRNQV"
     gene            323..1597
                     /gene="fahA"
                     /locus_tag="VFU15_04285"
     CDS             323..1597
                     /gene="fahA"
                     /locus_tag="VFU15_04285"
                     /EC_number="3.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009194695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004334 - fumarylacetoacetase activity
                     [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetase"
                     /protein_id="HEU4717022.1"
                     /translation="MLKANQPSLRSWVEVPPHSDFPIQNLPFGIFKTKTRGPRAGVAI
                     GEQILDLSAVAELGYFDMLAIDKKVFSKTVLNDFISLGKPVWRKVRERVSELLDEKNK
                     ALQSQAEARRQCLVPMSEAEMMMPVHIGDYTDFYSSIDHATNMGKMFRDPANALLPNW
                     KYIPVGYHGRSSSIMVSGCSFNRPKGQTKAPDAEAPSFGATKLLDFELETAFIIGKPT
                     TMGERISTESADDHIFGMVLFNDWSARDIQSWEYVPLGPFLAKNFCSVISPWIVTLDA
                     LEPFRVAGYKQEPEVLPYLAYKGDKNIDINLEVSIKAPGDGETIVCRSNYRYMYWTME
                     QQLAHHTVNGCNMNIGDLLASGTISGPDEGSFGSMMEITWRGTKPVKMADGTERKFIN
                     DGDTVIMRGYAEKDGVRVGFGECASKVLPAID"
     gene            1602..2417
                     /locus_tag="VFU15_04290"
     CDS             1602..2417
                     /locus_tag="VFU15_04290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717023.1"
                     /translation="MKYFFSLVAIALLSACGGDKTGDTHSPAKDSTPATVKTNPNTVI
                     LQTDSGQLEIETVNGKKQGHAWLRKDGKLIAEGSYDNDVPAGAWVNYGENGRVIKAFH
                     MSEGKKLFDLDSADFNFAKFQSRDFGAGFLVPEKWQSIKSPNPALLVSFTKKVPDTVK
                     VAPNMNVARGKLKPGENLQKLADLQLKMLHDAAGHVDVVQENLFTVDSCQSFQRYGMY
                     DMEDSRVGFLDAIIIHGDDVWFFSCEAQNKNQGEFLRYQGVFEKIVESFHRVN"
     gene            2504..4738
                     /locus_tag="VFU15_04295"
     CDS             2504..4738
                     /locus_tag="VFU15_04295"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013666261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEU4717024.1"
                     /translation="MAEKEVAPVIDTEKERKEILGRYRDLLRATKRRQEKGDRQIIRK
                     AFNVALEAHKDMRRKSGEPYIYHPIAVAKICAEEIGLGTTAVVCALLHDTVEDTDITL
                     DDIKGMFGEKVAKIIDGLTKISGVFDQQTNSLQAENFRKMLLTLSDDIRVILIKLADR
                     LHNMRTLDSMKRDKQLKIASETLYLYGPLAHRLGLNAMKTELEDLGLKFTEPEVYNDI
                     ARKLQETEPERKRFIQKFIQPINEILHEQGFKAKIIGRPKSIYSIYNKIRHKGVPFEE
                     IYDLFAIRIIIRTPQEKEKSDCWRVYSIVTDFYHPNPDRLRDWISTPKANGYESLHTT
                     VMGPEGKWVEVQIRTERMDELAEKGYAAHWKYKDGSPGSDSKESQLEDWLRRIREILE
                     SPEENALDFIDDFKLNLFAEEIFVFTPKGEMKTLPNQSTALDFAFEIHTKVGEHCIGA
                     KVNHKLVPLSHPLKSGDQVEILTSSKQSPKEDWLSYVITARAKAKIKNALKEEKRRIA
                     EEGREILERKFDHLKIDFTVQNINALQLFLKLPTAQDLFYRIAKGIIALKHLKAWQKE
                     KERPAKTAKSEPTLEQVVKDIRGKKAGDDALIIGDNLTKIEYKLSPCCNPIPGDDVFG
                     FITIGEGIKIHRVTCPNALQMMSNYAYRIVKARWTAQEQISFLAGIKVSGIDEVGLVN
                     NITRIISSELSVNMRSLSFDTHDGTFDGTIMLFVHDTQHLTELMKKLKKVHGVIAVTR
                     INPN"
BASE COUNT         1287 a         1299 c         1254 g          994 t
ORIGIN      
        1 tacctggttg cggtagcttg tgagataatt ggtctgaacg gtaagatcag gcccccacag
       61 cggcgttcct gtggcggtaa ccgaatccgc tacttcgaaa ttttcgtggt aaacgacagt
      121 ctgtgcattc agcgatgcga gcacaaacaa agcgcccgct gcgagtatga tttttttcat
      181 ggtaaattct attttgccag atatcaaata tacttaattg agggttgagg cctgtaatgc
      241 gatcgatata aatctccgcc cggaaaagcg tatcgccgct cgtacataaa cgctattttt
      301 ggcagcgcaa ccggaatcaa ttatgcttaa agccaatcaa ccttcacttc ggtcatgggt
      361 cgaagtccct cctcattctg atttccccat ccagaatctt cctttcggca ttttcaagac
      421 gaaaacccgc ggcccgagag ccggcgtcgc catcggggag caaatcctcg atctttccgc
      481 agtagccgag ctcggttatt tcgacatgct tgccattgat aaaaaagtgt tcagcaaaac
      541 cgtgctgaat gatttcatct cacttggaaa accggtgtgg agaaaagtgc gggaacgcgt
      601 ttccgaattg ctggatgaaa agaacaaggc gcttcagtcg caggcggaag cccgccggca
      661 gtgcctcgtg ccgatgagcg aagcggaaat gatgatgccc gttcacatcg gtgattacac
      721 tgatttctat tcgagcatcg atcacgcgac gaacatggga aaaatgttcc gcgacccggc
      781 gaacgcgctg ttgccgaact ggaaatacat tcccgttggt taccacggac gctcgtcctc
      841 catcatggtt tcgggctgca gtttcaaccg tccgaaagga cagacgaaag cgcccgacgc
      901 agaagcgccc tcgttcggtg cgacaaagct gctcgacttc gagctggaaa cagccttcat
      961 catcgggaaa ccgacgacga tgggcgaacg tatttccaca gagagcgcgg acgatcacat
     1021 tttcgggatg gtgcttttca acgactggag cgcacgcgac atacaatcat gggaatatgt
     1081 tccgctcgga cctttcctcg cgaaaaattt ctgctcggtc atttctccat ggatcgttac
     1141 gctcgatgcg ctcgaacctt tccgcgtggc gggctacaaa caggaaccgg aagttctccc
     1201 ctatctcgct tacaaaggcg acaaaaacat cgacatcaac ctggaggttt ccatcaaagc
     1261 gcccggcgat ggagagacga tcgtctgccg gtcgaattac aggtatatgt actggacgat
     1321 ggaacagcag ctcgcacatc acacggtgaa cggatgcaat atgaatatcg gcgacctgct
     1381 ggcttcagga acgatcagcg gacctgacga aggctctttc ggttccatga tggaaataac
     1441 gtggcgggga acaaaaccgg tgaagatggc cgacggaacc gaacgcaaat tcatcaatga
     1501 cggagatacg gtgatcatgc gcggctatgc ggaaaaagac ggcgtacgcg tcggtttcgg
     1561 cgaatgtgct tccaaagttt taccggcaat cgattaaacg catgaaatat tttttcagtc
     1621 ttgtcgcgat cgctcttctt tccgcctgcg gcggagataa aacgggcgac acgcattccc
     1681 cggcaaaaga ttccacgccg gcaaccgtaa aaaccaatcc caacaccgtg atcctgcaaa
     1741 ccgacagcgg tcagctcgag atcgaaaccg tgaacggaaa aaaacagggg cacgcatggc
     1801 tgcggaaaga cggaaagctc atcgcggaag gatcctacga taacgatgtg cccgcgggcg
     1861 cctgggtgaa ctacggcgaa aacgggagag tgatcaaagc gttccacatg tcggaaggga
     1921 aaaaactctt cgacctcgac agtgctgatt tcaattttgc aaaattccag agcagggatt
     1981 tcggcgccgg atttctcgtt cctgaaaaat ggcagtcgat caaatcaccg aaccccgcgc
     2041 tgctcgtttc attcaccaaa aaagttccgg atacggtgaa ggtcgcgccg aatatgaatg
     2101 tggcgcgcgg caagctgaaa ccaggggaaa acctgcagaa gctggccgac ctccagctca
     2161 aaatgctgca cgacgcggcg ggacatgttg acgtggtgca ggaaaatctt ttcaccgtcg
     2221 attcctgcca gagcttccag cgttacggga tgtatgacat ggaagactcc cgcgtcgggt
     2281 ttctcgatgc gatcatcatc cacggcgacg acgtgtggtt cttcagctgc gaagcgcaga
     2341 acaaaaacca gggagaattc ctgaggtacc agggcgtttt cgagaagatc gtggaaagtt
     2401 ttcaccgcgt gaactgatgt tcatttccct taccggatca attcgccggc cttttttttc
     2461 gtaacttcat tgtcgctatt tttcaatttg ctgaagttta attatggctg aaaaagaagt
     2521 cgctccggtt attgacacgg aaaaagagcg gaaagagata ctgggacgtt accgcgatct
     2581 gctgcgggct acaaagcgaa gacaggaaaa aggcgatcgc cagatcatcc ggaaggcatt
     2641 caacgttgcg ctggaagccc acaaggacat gcgtcgcaaa tcgggcgagc cgtacatcta
     2701 ccatcccatc gccgttgcga agatctgcgc cgaagaaata ggactgggca ctactgccgt
     2761 cgtctgtgca ttgctccacg ataccgttga agacaccgac atcacgcttg atgacatcaa
     2821 agggatgttc ggcgaaaagg tggcgaagat catcgacggc ctgacgaaaa tttcaggcgt
     2881 tttcgaccag cagacgaact cgctgcaggc ggagaatttc cgtaaaatgc tgctcacgct
     2941 ttcggatgat atccgcgtca tcctcatcaa gctcgcagac cgtcttcaca acatgcgcac
     3001 gctcgattcg atgaagcgcg acaagcagct gaagatcgca tcggaaacgc tttacctcta
     3061 cggtccgctc gcccaccgtc tcgggctcaa cgcgatgaag accgagctcg aagacctcgg
     3121 gctgaaattc acagagccgg aagtttacaa cgacatcgcc cgcaagctgc aggaaacgga
     3181 acccgaacgg aaacgtttca tccagaaatt catccagccg atcaacgaaa tactgcacga
     3241 gcagggattc aaggccaaga tcatcggccg gccgaagtcg atttattcca tttacaacaa
     3301 gatccgccac aaaggcgttc ccttcgaaga gatctacgat cttttcgcga tacgcatcat
     3361 catccgtacg ccgcaggaaa aggaaaaatc agattgctgg cgcgtgtatt ccatcgtcac
     3421 cgatttctac cacccgaatc ccgatcgcct ccgcgactgg atatccacgc ccaaagcgaa
     3481 cggttacgaa tcgctgcaca cgacggtgat gggcccggaa ggaaaatggg tggaagtgca
     3541 gatccgcacg gaacgcatgg acgagctcgc tgaaaaaggt tatgcggcgc actggaaata
     3601 caaggacggc agtcccggtt ccgacagcaa agaaagccag ctggaagact ggctgcgcag
     3661 gatccgcgaa attctcgaga gtcccgaaga aaatgcgctc gattttatcg acgacttcaa
     3721 gctgaatctt ttcgccgagg aaattttcgt ctttacgccg aaaggagaaa tgaagacgct
     3781 tcccaaccag tccaccgccc tcgattttgc ctttgagatt cacacgaagg tcggcgaaca
     3841 ttgtatcggc gcaaaagtga accacaagct tgttccgctc agccacccgc tgaaaagcgg
     3901 cgaccaggtg gaaattctta cgtcatccaa gcagtcgccg aaagaagact ggctctctta
     3961 cgtcatcacc gcccgcgcaa aagcgaagat caagaacgcg ctcaaggaag aaaaacggcg
     4021 catcgccgag gaaggacgcg agatcctcga gcggaagttc gatcatctca agatcgattt
     4081 caccgtgcag aacatcaatg cgctgcagct gttcctgaag cttcccaccg cgcaggacct
     4141 gttttaccgt atcgcgaaag ggattatcgc gctgaagcat ctcaaagcgt ggcagaaaga
     4201 aaaagagcgg cccgccaaaa cggccaagag cgaacccacg ctcgagcagg tggtaaaaga
     4261 catacgggga aagaaagcag gggacgacgc gctcatcatc ggcgacaacc tgacgaagat
     4321 cgaatacaag ctttcgccct gctgcaaccc gatccccggc gacgacgttt tcggattcat
     4381 caccattggc gaaggcataa agatccaccg cgttacctgc ccgaacgccc tgcagatgat
     4441 gtcgaactac gcctaccgta ttgtgaaagc gagatggacc gcgcaggaac agatctcgtt
     4501 cctcgccggc atcaaggtca gcgggatcga tgaagtcggc ctggtgaaca atatcacccg
     4561 catcatctcc agcgagctga gcgtgaacat gcgttcactg agctttgaca cgcacgacgg
     4621 aacgttcgac gggacgatca tgctcttcgt gcacgatacg cagcacctta ccgagctgat
     4681 gaaaaagctg aagaaagtgc acggcgtgat cgcggttacc cggatcaatc cgaactgaga
     4741 ttttacgcct ttttccttcg cgcctttccc cttttttatt cctaaattta cgctccttaa
     4801 aaaccgctgt ccatgacagc ttcagaagtc agag
//
LOCUS       DASTSF010000146         3777 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1396031,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000146 DASTSF010000000
VERSION     DASTSF010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3777
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1396031"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..408)
                     /locus_tag="VFU15_04300"
     CDS             complement(<1..408)
                     /locus_tag="VFU15_04300"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEU4717025.1"
                     /translation="MSKRLAYLPRLDSLRAIAALMVLFVHYLSLKKSTFAYGGNGVEI
                     FFVISGFLITGILLSQKNETSLPRHRLFGSFVVKRALRLFPAYYLYLAFLYVLHATGG
                     LWLCNKGDFWHYLTYTQNFLFLQKPGQSSLLFHT"
     gene            complement(420..1274)
                     /locus_tag="VFU15_04305"
     CDS             complement(420..1274)
                     /locus_tag="VFU15_04305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717026.1"
                     /translation="MKKFLFVTHITPCAKRTALRRSLIDIYFRALDAQTYGNWKVAVL
                     GEEEKTEGRFHYFFLGDLPKEEKFAEVRKIFSKPEMTALTGEADYIIKLDDDDIISPR
                     LLQDLAEFDGDLYYDKYHTFTDVSSGMITQQRRQWIASTCVHKKEHALSAWNGPGASA
                     VGNLLYSDHSQSWHLYYGDKKTVAADPHHPVYLRVLSPTSVSSGASAGEGNRNDIPMN
                     GYRDYLAKFGDWTRADVKDFDAYLPLLARAWNDFSGSPQSPLPPFAKKEGGVRGRIRK
                     WLSGLHKS"
     gene            complement(1281..2336)
                     /locus_tag="VFU15_04310"
     CDS             complement(1281..2336)
                     /locus_tag="VFU15_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008352623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HEU4717027.1"
                     /translation="MKNILEFIDHYDPAIIQRPYIIAEAGVNHEGSMDLAKRLIDEAA
                     DGGADAIKFQTYKADTIASKDSPYYWDITKEPTRSQHELFKKYDKFWKKEYEELKNYC
                     DKAGIEFMSTPFDVESANFLNDMMPVYKISSSDITNLPFIEHMCRFGKPILLSTGSSF
                     HWEIQQALDTIEKFGNKVVLLHCILNYPTADENANLGMLIDQKVHYPQTIPGYSDHTL
                     PGKMEVLKIATLLGAAVLEKHFTHDKTLPGNDHYHAMDRDDLKHFHREMDFVFKVLGD
                     FKKHPLASEEKSRANARRSLVALKNIKSGEAVTRDHLTWKRPASGISPKDIEQLIGKK
                     ALRDISEDEVLKWNMFS"
     gene            complement(2348..3175)
                     /locus_tag="VFU15_04315"
     CDS             complement(2348..3175)
                     /locus_tag="VFU15_04315"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU4717028.1"
                     /translation="MKNQERTTDPSAIDFRKKFELTDKVIVITGACGLIGRAFAEACA
                     QFGAHIVLADIAVSAPEEKAAELEKRHGRKMLGVIVDVAKKNSVEDLKNKTLAAFGRI
                     DGLVNAHQNKSHLIFEPFEKVSEENWDTVVEVNLKGTFLLCQVIGSYMAEQGKGAIIN
                     IPSTYSVVAPNQNLYKGTSLGCPAAYSASKGGIEALSQYLATYWASKGVRVNQITPHG
                     VWNNHEEQFEKNFANFSPLQRMSYNHEVADAAVFLLSDASSYVTGHNLLVEGGWTAW"
     gene            complement(3178..>3777)
                     /locus_tag="VFU15_04320"
     CDS             complement(3178..>3777)
                     /locus_tag="VFU15_04320"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEU4717029.1"
                     /translation="AGSITKFGVVPDMATWGKGIANGFSFCALTGKKEVMELGGIRRK
                     GEQKLFLVSTTHGGETCSIAAGLATIAEFEKNDVIGHNHRMGEKFIAGTKELLTKHGL
                     TGYIDQLQFSWHASLGFRNAKKEADFGMRTLFNQEVIKRGVLFQGIFCPCFSHTDEDM
                     AHILWAFDEAMEVYKKALEDGYEKYLTGDVIKPVFRKII"
BASE COUNT          837 a         1006 c          980 g          954 t
ORIGIN      
        1 cgtatgaaac agcagcgagc tttgtcctgg tttctgaagg aaaagaaaat tctgcgtgta
       61 cgtgaggtaa tgccagaaat cgcctttatt gcaaagccac agtcccccgg ttgcatggag
      121 cacgtaaaga aaagcgagat acaggtaata cgccgggaaa agccggagcg ctcttttcac
      181 aacgaagctc ccgaaaagcc ggtgacgcgg caaagacgtt tcgttcttct gagaaagaag
      241 aattcccgtg atcaggaatc ccgagatgac aaaaaatatc tccacgccgt tcccgccata
      301 ggcaaacgtg cttttcttca gcgagaggta atgaacgaaa agtaccatca gcgctgcgat
      361 ggcgcgcaat gaatcaagtc gcggaagata agcgagacgt ttgctcatgg atcagcaact
      421 cagcttttat gaagacccga gagccattta cggatcctgc cgcgcacgcc gccctctttc
      481 ttcgcgaaag gcgggagagg cgattgcggg gaacctgaaa aatcgttcca cgcacgggcg
      541 agaagaggaa gataagcatc gaaatctttt acatcggcac gggtccagtc tccgaatttc
      601 gcgaggtaat cccggtatcc gttcatcggg atatcattgc ggttcccctc cccggctgaa
      661 gctcccgatg agactgaagt cggggaaagc acgcgcaggt agaccgggtg atgcgggtcg
      721 gccgccacgg tctttttgtc gccgtaatag agatgccagc tctggctgtg atcggaatac
      781 agcaggttgc cgacggcaga agccccgggt ccgttccacg ctgaaagcgc atgctctttt
      841 ttatgcacgc aggtgctcgc gatccactgc cttcgctgct gcgtgatcat cccgctggaa
      901 acatcagtga acgtgtggta tttatcgtaa taaagatcgc cgtcgaattc cgcgaggtcc
      961 tgcagaagac ggggcgaaat gatatcgtcg tcgtccagct tgatgatgta atccgcttca
     1021 ccggtaagcg cggtcatttc cggcttcgaa aagatcttcc gcacctctgc aaatttctcc
     1081 tccttcggaa gatcgccgag aaaaaaataa tgaaagcgtc cttcggtctt ttcttcttca
     1141 cccagcacgg cgactttcca gttgccgtac gtctgcgcat cgagggcgcg gaaataaata
     1201 tcgatcagcg accgccgcag cgcagtgcgt ttggcgcagg gagtgatgtg tgtgacaaaa
     1261 agaaatttct tcatccgcgc tcagctgaac atattccatt tcagcacctc gtcctctgaa
     1321 atatcgcgca gcgccttctt cccgatcagc tgctcaatgt ctttcgggct gatgccgctg
     1381 gccgggcgct tccaggtgag gtgatcgcgg gtgacggctt cgccggactt aatattcttc
     1441 aacgccacaa ggctgcgtct tgcgttcgca cgcgattttt cttcagaagc aagcggatgt
     1501 tttttgaaat caccgagcac tttgaaaacg aaatccatct cgcggtgaaa atgcttcagg
     1561 tcgtccctgt ccatggcgtg gtaatggtcg ttgccgggca atgttttgtc gtgcgtaaaa
     1621 tgcttttcca ggacagccgc gccaagcagc gtggcgatct tcagcacttc catttttccc
     1681 gggagcgtat gatcggaata acccgggatg gtttgcgggt aatggacctt ctggtcgatg
     1741 agcatgccga ggttcgcatt ctcgtccgca gtgggataat tcaggatgca gtgcagcaaa
     1801 acgactttgt tcccgaactt ttctatcgtg tcgagcgcct gctggatctc ccagtgaaaa
     1861 gaagaaccgg tcgaaagcag gatcggtttt ccgaaacggc acatgtgttc gataaaagga
     1921 agattggtga tgtcgctcga agagattttg tacaccggca tcatgtcgtt caggaagttc
     1981 gccgattcca catcgaacgg cgtcgacatg aattcgatcc cggccttgtc gcaatagttc
     2041 ttcagctctt cgtattcctt cttccagaac ttgtcatatt ttttaaaaag ttcgtgctgg
     2101 ctgcgtgtag gttccttcgt gatgtcccag taatacgggc tgtctttgct ggcgatcgta
     2161 tcggctttgt acgtctggaa cttgatcgca tccgctccgc cgtccgcagc ttcgtcgatg
     2221 agacgtttcg caagatccat cgaaccttcg tgattgacgc cggcttccgc aatgatgtac
     2281 ggacgctgaa tgatcgctgg atcataatga tcgatgaact ctaaaatgtt tttcatagcg
     2341 tttgttttta ccaggctgtc catccgcctt cgacgagaag gttatggccg gtgacatagg
     2401 aagaagcgtc gctgagaagg aaaaccgcgg cgtccgccac ttcatggtta tagctcatgc
     2461 gctgcagggg actgaagttc gcaaaattct tttcgaactg ttcttcgtgg ttgttccaga
     2521 caccgtgcgg cgtgatctgg ttgacacgca cgcctttcga cgcccagtac gtggcaaggt
     2581 attgtgaaag cgcttcaatg ccgcccttcg aagcagaata ggcagcggga cagccgaggc
     2641 tcgttccttt gtacaggttc tggttcggtg caacgacgct gtaagtagaa ggaatattga
     2701 tgatcgcacc tttcccctgc tctgccatgt acgagccgat gacctggcac agcaggaacg
     2761 ttcctttaag gttcacttcc acgactgtat cccagttttc ctcgctcact ttttcgaatg
     2821 gttcgaaaat gagatgtgac ttgttctggt gcgcattgac aagtccgtca atccttccga
     2881 atgcggcaag cgttttattc ttcaggtctt ccacggaatt ctttttcgcc acatcgacaa
     2941 taacgccgag catctttctt ccgtgacgtt tctccagctc cgccgctttt tcttccggtg
     3001 cggaaacagc gatatccgcc agcacaatat gcgcgccgaa ctgcgcacag gcttctgcaa
     3061 acgcacgacc gatcaacccg caggcgccgg tgatgacgat cactttatcc gtcagctcga
     3121 attttttccg gaaatcgatc gcagaaggat ctgttgtccg ttcctggttt ttcatcgtca
     3181 gataattttc cggaaaacag gtttgatcac atccccggtg aggtattttt catacccgtc
     3241 ttccaatgct tttttataca cttccatcgc ttcgtcgaac gcccagagaa tgtgcgccat
     3301 gtcttcatcc gtatgactga agcagggaca gaaaattccc tggaacaaaa cgccgcgttt
     3361 gatcacttcc tggttgaaca gcgtgcgcat gccgaaatcc gcttctttct tcgcattgcg
     3421 gaaaccgagg ctcgcatgcc agctgaactg cagctggtcg atgtagcccg taagcccgtg
     3481 cttagtgaga agctcctttg tcccggcgat gaacttctcg cccatccggt ggttgtgccc
     3541 gatcacgtca ttcttttcaa actcggcgat cgtggcaagc ccggccgcga tcgagcaggt
     3601 ttccccgccg tgcgtagtgg agacgaggaa caatttctgt tctcccttgc ggcgtattcc
     3661 gccgagctcc atcacttctt ttttcccggt gagggcgcag aatgaaaatc cgttcgcgat
     3721 gccttttccc cacgtagcca tgtcgggaac gacgccgaat ttcgtgatcg atccggc
//
LOCUS       DASTSF010000147        10956 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1281883,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000147 DASTSF010000000
VERSION     DASTSF010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 10956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10956
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1281883"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..247
                     /locus_tag="VFU15_04325"
     CDS             <1..247
                     /locus_tag="VFU15_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEU4717030.1"
                     /translation="ISHDPVLHANVAMISITIGITLVGVVLWGTLMGSLLPLFLKRLG
                     LDPAASSAPFVATLVDVTGIVIYFSFAMFLLHGSLLK"
     gene            248..1189
                     /locus_tag="VFU15_04330"
     CDS             248..1189
                     /locus_tag="VFU15_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007647612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU4717031.1"
                     /translation="MASVLFLDTNHPLMITMLREKGYEIREEYAASRDEVMKMIPSFD
                     AIVIRSRFKIDREFIDAGKKLKCIGRVGAGMENIDVAYAEGKGIRCLHAPEGNRDAVG
                     EHATGMLLALMNNLLRADREVRGGKWNRESNRGYELQGKTVGIIGYGNMGSAFAQRLR
                     GFGVKILAYDKYKNGFGDAFVNESSLEEIFAEADVVSLHLPLTEETFHLCDEKFFASF
                     RKPVWFVNTARGKNVKTEALVQALENGKVKGACLDVLEYESVSFEAIDPKTLPPAFHY
                     LVHSEKVILSPHIAGWTFESHEKLAKFLAEKMIAVLG"
     gene            complement(1190..1582)
                     /locus_tag="VFU15_04335"
     CDS             complement(1190..1582)
                     /locus_tag="VFU15_04335"
                     /inference="COORDINATES: protein motif:HMM:NF020903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1987 domain-containing protein"
                     /protein_id="HEU4717032.1"
                     /translation="MNEAALMQDLTIRQTIRTAGVQFSAGSAYLSIQGNSIPENSDGF
                     FQPLHDWVEEFRNVNSQPVTFRIFMTYFNTATIRHLIAIMKKLIQTYGSNLKIEWAYE
                     KGDEEIRDRGQDISEVVKFPFVFDEVAV"
     gene            complement(1660..4827)
                     /locus_tag="VFU15_04340"
     CDS             complement(1660..4827)
                     /locus_tag="VFU15_04340"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HEU4717033.1"
                     /translation="MRPCRFFAVFLLFFTGVTRLVLSGQTVANYTFTPYTGIYTQLQG
                     TAGSVSPSLSGGSLDDGWFNSVPIGFSFTYEGVSYTTLSASTNGWFTFGSTLSSSAYV
                     NNLSTGTPRPVVAPLWDDLAMGATGDFTYYTTGVTPNRVFTVEWLNMKWNYATTTPSV
                     SFQVKLFESNRRIEFVYRQEAGTPATTSASIGLAGVTSGVFLSLNGTGTSPAASSGTE
                     TTTLSTSPSTGRTYRFDSYKIRPVITGVTPLASPLGTTVSISGSGFEPNTAGNTVYFG
                     ATKATVLTSGTTAMTVTLPSGALYKPVSETNSDHLTGWYTKPYDVTFPCQGYSAGNMI
                     IQGSPVNFTAGNAPSDVAIGDIDRDGKPDLVVTNQSSNTVSVYRNISTMGSMSTSSFA
                     PKVDFTTGNSPCSVAVSDLDGDGKLDVIVSNNGSSTVSVLRNTSTTGVINSASLAAKV
                     DFATTSAPKGLDIADMDQDGKPDILVVCNVSPYKLDIFRNVSTSGSITTASFSAAVSF
                     ATSTGPVDVSTGDIDGDGIPDVVCTDFGASRIEIWRNTSVAGTFSFGVASTQFTLSQP
                     VAAKVSDLDGDGKADIAIANQGDWHMSVYQNFSTTGSFSFSARHDYYATNGQNDLNID
                     DINGDGKPEIIITGQSNTGIGVYTNNTTVGSIGTASFGSCYYYYVASGTLVSTASGDL
                     NDDGKSDIIATDASTNDFAIFPNQWSQFINVEPNTCTGSTSYCYDGTWKVYFHPVYQN
                     RALFAVEEKGTSLGLTVDEYKNASPGTYNGMRFLARHFRITPTVQPPVPVRIRLFYLP
                     AELTALEAVDPTVTSPSSLSVTQYDGPTEDATYNPTDATSLTWIPQTSITTGTAYGGN
                     YLEFTVSNFSEFWIHGGGGVLPIELLDFNATPVDNTSVKLTWSTASETNNDFFTVEKT
                     RDGTNFETVTTVDGAGNSTQTLHYETVDEHPYDGLSYYRLKQTDFNGHATYSGVVPVE
                     FDNGGALFNMYPNPSDGTVHFTFPGDPEEEVTLRVFDLSGREVSTKTAAASSLMSDVE
                     IFEPGIYIVRIEKGGKQFCRRLVVN"
     gene            5078..7150
                     /locus_tag="VFU15_04345"
     CDS             5078..7150
                     /locus_tag="VFU15_04345"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing sensor
                     histidine kinase"
                     /protein_id="HEU4717034.1"
                     /translation="MRKPILFTTLFTILLLHLFAGAGKKLDSLVHALPQRHDTMRVVM
                     LIKISQYYMGVDDDSALFYAKMLKQEAEKWGKPSRIADAYLILGSCYYHLRQDDSAFH
                     YKNLSKEIAEKNDYKKGIANAYSDLGLYLKERGEMDSAVTFMLRSATIREEIGDERNL
                     AVSYTNLGLIFASLDENEKAAYYDRKALAIFKKNRQMSSYALVLNNLASVYDGWKKYD
                     TALAMYHLSYEVRDSLHDTYGMAQSLLNIGVTYRNLGDFAGAEKNLSDALALKTKIAD
                     SSGIAQSMLDLAQVYRLEKKSDRSVPLLQHAEAIARNSPDMKELRRDIYREFSDLYAD
                     LHRYDLALQYRTKYEELRDSIFDAAKLKVISEMQTKYETAKKEKDILRLQEEKNQKDI
                     RIARDENRQLYLYGGLAVLGIVVLFALFAFFSQRSNSRSLSEKNAQISRQNSTLKELN
                     KKLIESEEELTKLNATKDQLFSVISHDLSGPVKAIGNFSTAIAGSEASMSKEELSASV
                     AKLGNAIFPVQSLLDNLLHWSLLQRNAGEPRFTEFNAPELLHEVKSIFSAALETKKIN
                     VEISADAQLLIRSDRNICSLIVRNLLGNAIKFSPAGSRIVLAARRVNEKMVFSCRDSG
                     PGISAERITAILAGKNVQPEKGTSAESGTGLGLKLVQEYLRVLGGKLLIVSDGKNGAT
                     FEAEIPVL"
     gene            complement(7171..7368)
                     /locus_tag="VFU15_04350"
     CDS             complement(7171..7368)
                     /locus_tag="VFU15_04350"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEU4717035.1"
                     /translation="MAQLSPRELEILVLVCSDKSSREIAEELCISAATVDTHRKALLR
                     KTGSKNKIGLVKFALRNRLIG"
     gene            complement(7403..8080)
                     /locus_tag="VFU15_04355"
     CDS             complement(7403..8080)
                     /locus_tag="VFU15_04355"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU4717036.1"
                     /translation="MQKPYRIALADDHPLYLEGLEILLNKSGEAKVVATAKDGDEAIA
                     MMEKTKADLLLLDLHLPGRNGIEVAEALSRNHSAVKIVMLTMQRGGRYVDKLRKLGVR
                     GYLLKSVSLDELLDAVRKVMSGGEHFSEDIIRYDHEENMRLKSSAIVDDSPGTLLTAR
                     EREILVLVCKEFSSAEIGKKLFISTGTVDTHRKNILIKLGVGNTVGLVKYALKHGLLD
                     ENTGIPD"
     gene            complement(8083..9354)
                     /locus_tag="VFU15_04360"
     CDS             complement(8083..9354)
                     /locus_tag="VFU15_04360"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003006083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEU4717037.1"
                     /translation="MKIFKFGGASVKDAGAVKNVAEIIRLFPSDNLVIVISAMGKTTN
                     ALEKLAEAFFFKKDDPVKILTEIKGFHLAILSELFPDKTHPVYDEIHNTFVELEWATE
                     DEPSGDFDREYDQIVSVGEILSTRIVSAYLNSAGVNAQWRDVRDFIKTDNTYREGKVD
                     WDLTEKAVDASLVPFFTEKKGKIVVTQGFLGVTSENYTTTLGREGSDYTAAILAWCTN
                     AESVTIWKDVPGVLNADPKWFDDTRLIPRLTYQDAIELTYYGATVIHPKTIKPLQNKN
                     IPLFVRSFLDPAKEGTKIHDAHVPLPIPCFIFKVNQVLLSISPKDFSFIVEENLGDIF
                     DYFAEFRVKINVMLNSAISFSVSVDSDEKKLPKLIDRLKENFRVLYNENVELVTIRYY
                     DQPTIDRVTTAKQVLLEVKSRYTTQIVMKNR"
     gene            complement(9485..10594)
                     /locus_tag="VFU15_04365"
     CDS             complement(9485..10594)
                     /locus_tag="VFU15_04365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717038.1"
                     /translation="MKNILFAAVIAVLPACLAAQDTPVLTDTDLGKTPGWAKIELDSS
                     FQKTTNGGVYEFYDLLNSKGKSNCIYYGKWVFPQDAAKVIYTKTDPSSSSKLADGTAV
                     FYDAAGLRIAKYVMKGGFILLEYNYRWKKSKKERNGSLHFSLQFKPAKDSVEVIYNVV
                     ERGDLVMGFHASYNGNKWKTIPGPIGFVPEDENFIGGVWQPYEYSAYQYDMHIDATAH
                     NGKNAATIRSLVPKPGGSGGLVQIIRADQYLGKRVRMTAWMKSENVSNFAGLWLRVDQ
                     AAPEGKNIVCFDNMRDRPVKGTTGWTKYEIVFDVPPGATDIVFGAMLQGGTGQIWFDD
                     FSFEVVDNSVPLTTETDITPARPLSSSPANLDFEK"
BASE COUNT         2473 a         2788 c         3085 g         2610 t
ORIGIN      
        1 catttcgcat gatcctgtcc tgcatgctaa cgtggcgatg atctcgatca cgatcggtat
       61 tacgctggtg ggcgtcgtgc tgtggggaac gctcatggga tcgctgctcc cgttattcct
      121 caaacgtctc ggtctcgacc ccgcggcatc gtccgctcct tttgttgcga cgcttgtcga
      181 cgtgacaggc atcgttattt atttttcatt cgcgatgttc ctgctgcacg gatcacttct
      241 caaataaatg gcttccgtac tttttctcga tacgaatcac ccgctcatga tcacgatgct
      301 gcgtgaaaag ggatatgaga tccgcgagga atatgctgcg tcgcgggatg aagtgatgaa
      361 gatgatccct tcattcgacg cgattgtcat ccgcagccgc tttaagatcg acagggaatt
      421 catcgacgcg ggaaaaaagc tgaaatgcat cgggcgcgtc ggtgcgggaa tggaaaacat
      481 cgacgtggcg tatgcagaag gaaaaggcat ccgctgtctt catgcgccgg aaggaaaccg
      541 cgatgcggtg ggtgagcatg cgaccggaat gctgctggcg ctgatgaata acctgctgcg
      601 tgcagatcgc gaagtgcgcg gggggaaatg gaaccgtgaa tcgaaccgcg gctacgagct
      661 gcagggaaaa acggtgggca ttatcggtta cgggaatatg ggttcggcgt ttgcgcagcg
      721 tttgcgcggg ttcggtgtga aaatacttgc ttacgataaa tacaaaaacg gtttcggcga
      781 tgcgttcgtg aacgaatcct cgttggaaga gattttcgcg gaggctgatg tggtcagcct
      841 gcacctcccg ctgacggaag aaacattcca cctctgcgat gaaaaatttt tcgcgtcttt
      901 ccgcaagccg gtttggttcg tgaataccgc gcgcggaaaa aatgtgaaaa cggaagcgct
      961 cgtgcaggcg cttgaaaacg ggaaagtgaa gggcgcctgc ctcgacgtgc tcgaatacga
     1021 aagcgtttca ttcgaagcga tcgatcccaa aacgctcccg cctgcgttcc attatctcgt
     1081 tcattcggaa aaagtcatcc tttccccgca cattgcggga tggacgttcg aaagccacga
     1141 aaaacttgcg aaattccttg cggaaaaaat gatcgccgtg ctcgggtgat cagacggcga
     1201 cctcgtcgaa tacgaaagga aatttaacca cttcggaaat atcctgcccg cggtcgcgga
     1261 tctcttcgtc gcctttttcg tacgcccatt cgatcttcag gttgctgccg tacgtctgga
     1321 tcagcttttt catgatcgcg atgaggtgtc ggatcgtggc ggtattgaaa tacgtcatga
     1381 aaatgcggaa tgtcacgggc tgagaattca cattgcggaa ttcttccacc cagtcgtgca
     1441 aaggctgaaa aaaaccgtct gaattttccg ggatggaatt tccctggatg ctgagataag
     1501 cggatcccgc gctgaactga acgccggcgg tgcgaatcgt ttgtctgatc gtgagatcct
     1561 gcataagagc tgcttcattc aaagatcgta aatagaaatc aaatatttat aaaaaaatag
     1621 tttatagctg tatgcgcatt tgcaggtgat aaatgcagct caattgacga caagcctgcg
     1681 gcagaattgt ttcccgcctt tttcaatgcg cacgatgtaa atgcccggtt cgaatatttc
     1741 tacatccgac atgagcgatg atgctgccgc ggttttcgtg ctcacttcgc gcccgctcag
     1801 gtcaaacacg cggagcgtca cttcttcttc cgggtctccg gggaaggtga agtgaaccgt
     1861 tccgtccgag gggttcgggt acatgttgaa cagcgcaccg ccattgtcga attcaaccgg
     1921 aacaacgccg gaatatgtgg cgtgcccgtt aaagtccgtt tgcttcaggc ggtaataaga
     1981 aaggccgtcg tacggatgtt catcaaccgt ttcgtaatgg agcgtctgcg tgctgttgcc
     2041 cgcaccgtca acagtcgtca cggtttcaaa atttgttccg tcccgcgttt tttcgacggt
     2101 gaaaaaatcg ttgttcgtct ccgatgcggt cgaccacgtg agcttcaccg atgtgttgtc
     2161 aaccggcgtc gcattgaaat caagcaattc gatcgggaga acgccgccac ctccgtggat
     2221 ccagaattcc gaaaaattgc tgacggtgaa ctcgaggtaa ttcccgccgt acgcggtgcc
     2281 cgtcgtgatc gatgtctgcg gtatccatgt gagtgaagtg gcgtcggtgg gattgtaggt
     2341 tgcgtcttct gttggtccgt cgtattgcgt gacggaaaga ctggaaggag aagtgactgt
     2401 gggatcgaca gcttccagcg cggtgagttc ggcgggaaga taaaaaagac ggatgcgtac
     2461 cggcaccgga ggttggactg taggcgtgat ccggaaatgc cgcgcgagaa aacgcatgcc
     2521 gttatacgtt ccgggtgaag cgttcttgta ttcatccacc gtaagaccga gactggttcc
     2581 tttttcttcg acggcgaaca gcgccctgtt ctggtaaacg ggatggaaat aaactttcca
     2641 cgttccgtcg tagcaatagg acgtgctccc ggtgcaggtg ttcggttcga cgttgatgaa
     2701 ctgggaccac tggttgggaa agatcgcgaa atcatttgtg ctggcatcgg tggcgatgat
     2761 atcggatttg ccgtcatcat tgagatcgcc gcttgccgtg ctcacgagtg ttccggacgc
     2821 aacgtaataa taatagcagc tgccgaaaga tgctgtaccg atggaaccca ctgtagtgtt
     2881 gttcgtgtac accccgatgc cggtattgga ttggccggta ataatgatct cgggttttcc
     2941 gtcgccgttg atgtcatcga tgttcaggtc gttctggccg ttcgtggcgt agtaatcatg
     3001 acgtgcagaa aaactgaagc tgcccgtggt ggaaaaattc tggtacacgc tcatgtgcca
     3061 gtcgccctgg ttggcaatgg cgatatccgc ttttccatcg ccgtcgagat cggaaacttt
     3121 cgcagcaacg ggttgcgaca acgtgaactg cgtggatgcc acaccgaatg aaaatgttcc
     3181 tgcgacgctg gtgttccgcc agatctctat gcggctcgcg ccgaaatccg tgcagacgac
     3241 atccggtatt ccgtctccgt cgatgtcgcc ggtcgaaaca tccacgggtc ccgtggaagt
     3301 ggcgaatgaa accgcagcgg agaaagaagc cgtggttatg ctgcccgacg tgctcacgtt
     3361 cctgaaaata tccagcttgt acggcgagac gttgcacacc accaggatgt cgggtttccc
     3421 gtcctggtcc atgtcggcga tgtcgagtcc tttcggcgcc gacgtcgtgg cgaaatctac
     3481 tttcgctgca agcgatgcgg aattgataac gcctgttgtg ctggtgttgc gcaggacaga
     3541 cacggtggaa gagccgttgt tcgacacgat cacgtcgagc ttgccgtccc cgtcgaggtc
     3601 gctgaccgca acggagcagg gactgttacc ggtggtaaaa tccactttcg gcgcaaatga
     3661 agaagtgctc atgcttccca tcgtggaaat attccggtaa acggaaacgg tgttgctgct
     3721 ttggttcgtc acgacaagat ccggtttccc gtccctgtcg atatcaccga tggcgacatc
     3781 cgaaggtgca ttgcccgctg tgaagtttac cgggcttccc tggatgatca tgttcccggc
     3841 gctgtagccc tggcagggaa aagtgacatc gtacggtttc gtgtaccagc cggtgaggtg
     3901 gtcgctgttc gtttcgctca ccggtttgta aagtgcgccc gaaggaagcg tcacggtcat
     3961 ggcggtggtg ccggaagtca gcaccgtagc tttcgttgcg ccgaaataaa cggtgttgcc
     4021 cgcggtgttc ggttcgaagc cggaaccgga aatggaaaca gttgttccga gcggcgaggc
     4081 aagcggggtg acgccggtga tgaccgggcg gatcttgtag gaatcgaaac ggtaggtgcg
     4141 gccggttgac gggctcgtgg aaagcgtagt ggtttctgtt ccgctgcttg cagccgggct
     4201 cgtgccggtt ccgttcagcg aaagaaaaac accggatgtc acgccggcga gcccgatcga
     4261 agcggaagtg gtggccggtg ttcccgcttc ctggcggtaa acgaattcaa tgcgcctgtt
     4321 ggattcgaac aattttacct ggaaagaaac ggaaggagtc gtggtcgcgt agttccattt
     4381 catgttcagc cattccaccg tgaaaacgcg attgggtgta acgcctgttg tgtaataagt
     4441 gaaatcgccc gtggcgccca tggcaaggtc gtcccacaat ggcgcgacga caggacgcgg
     4501 tgttcccgtg gaaaggttgt tgacgtaagc ggaagatgaa agcgtgctgc cgaatgtgaa
     4561 ccagccgttc gtgctcgcgg aaagcgtcgt gtagctgacg ccttcataag taaaggagaa
     4621 accgatcgga acggaattga accacccgtc gtcgagcgaa ccgccggaaa gagaaggaga
     4681 aaccgagcct gccgtgccct ggagctgtgt ataaatcccg gtgtacgggg tgaatgtgta
     4741 attggcgacg gtctggccgc tgagcaccaa ccgggttaca cccgtgaaaa aaagcaggaa
     4801 aacggcgaaa aagcggcagg gtctcatggg atttatttac cggcgggaac gaacagccag
     4861 atcagtgcgg ccgcgatgat cacggcgacg acggtgatgc ggaagatccg tgcgtggaat
     4921 tcagaataga ctttgatgcg tggatgagac atgagagctg tttttgctga tcaaagttga
     4981 tcctaaatca tgccatggac aatcctgtat tccggtattt tcccgtctga gcggaagcct
     5041 gttttttatt tacccggaat gcttgtatta ttgattcatg cggaaaccga ttcttttcac
     5101 gacccttttc acgatcctgc tgcttcacct tttcgcaggt gcaggaaaaa agctcgacag
     5161 ccttgttcat gcgcttcctc agcggcacga taccatgcgc gtggttatgc tgatcaagat
     5221 ctcgcagtat tacatgggcg tcgacgatga cagcgcgctg ttctacgcga aaatgctgaa
     5281 gcaggaagcg gaaaaatggg gaaagccttc gcgcattgcc gacgcttatc tcatcctcgg
     5341 atcgtgctat tatcacctgc ggcaggatga ttccgcattt cattataaaa atctttcgaa
     5401 ggagatcgcg gaaaagaacg attacaaaaa agggatcgcc aatgcataca gcgatctcgg
     5461 actttacctg aaggaacgcg gtgaaatgga ttcggcggtg accttcatgc tgcgttccgc
     5521 cacgatcagg gaagagatcg gcgatgaacg caatctcgcg gtttcctata cgaacctcgg
     5581 cctcattttc gcttcgctgg atgagaatga gaaagccgcg tattacgacc ggaaagcgct
     5641 ggctatattc aaaaagaaca gacagatgag cagctatgcg ctcgtgctca ataacctcgc
     5701 ttcggtttac gacggatgga aaaaatacga tacggcgctc gccatgtatc acttgtcgta
     5761 cgaagtgcgc gacagcctcc atgacaccta cggcatggcc cagtcgctgc tgaatatcgg
     5821 cgtgacgtac cgcaacctcg gtgattttgc gggtgcggaa aaaaatcttt cggatgcgct
     5881 tgcactgaaa acgaagatcg cggactcttc cggcattgcc cagtccatgc tcgatctcgc
     5941 gcaggtgtat cgcctggaaa aaaagtctga caggtcggtt ccgctgctgc aacacgcgga
     6001 agcgatcgcg cggaacagtc ccgacatgaa agagctgcga cgcgacattt accgggagtt
     6061 ttcagacctg tatgccgacc tgcaccggta cgacctggcc ctgcagtacc ggacgaaata
     6121 cgaggagctg cgcgacagca ttttcgacgc ggccaagctg aaggtcattt cggaaatgca
     6181 gacgaaatac gagacggcga aaaaagaaaa agatattctc cgcctgcagg aggaaaaaaa
     6241 ccagaaggac atacgcatcg cccgcgacga gaaccggcag ctgtatctct acggtggact
     6301 ggccgtgctc gggatcgtgg tccttttcgc cctttttgcg ttcttttccc agcgcagcaa
     6361 ttcaagatcg ctcagcgaaa aaaacgcgca gatctcgcgc cagaattcca cgctgaaaga
     6421 gctcaataaa aaactgatcg aatcggaaga ggagctgacg aagctgaacg caacgaaaga
     6481 ccagctgttt tccgtcatct cccacgatct ttccgggccg gtgaaagcga tcgggaattt
     6541 cagcacggct atcgcgggaa gcgaagcgtc catgtcgaaa gaagagcttt ccgcttccgt
     6601 tgcaaagctt ggcaatgcga tcttccctgt acagtcgctg ctggataacc tcctgcactg
     6661 gtcgctgctg cagcggaatg ccggagagcc gcgcttcacg gaattcaatg cgccggaatt
     6721 gcttcacgaa gtgaaaagca ttttcagcgc agcgctcgaa acgaaaaaga tcaatgtgga
     6781 gatcagcgct gatgcgcagc tgctcatccg ttccgaccgc aacatctgca gcctcatcgt
     6841 gcgcaacctg ctcggcaacg cgatcaagtt ttccccggcg ggatcacgca tcgtgcttgc
     6901 tgcccggcgc gtgaatgaaa aaatggtttt ctcctgccgt gattccggcc cgggaatttc
     6961 cgccgaaagg ataacggcga tcctcgccgg aaaaaatgtg cagccggaaa aaggaacatc
     7021 ggcggaaagc gggacagggc tcgggcttaa gctcgtgcag gaatatctcc gggtgcttgg
     7081 cgggaagctg ctcatcgtat cagacggaaa gaacggcgca acgttcgaag cggagatccc
     7141 ggttttgtga gcctgcgcct ctttccgcat tcaaccgatc agcctgttcc tcagcgcaaa
     7201 cttcaccagc ccgatcttgt tcttcgaacc ggttttccgc agcagcgctt tgcggtgcgt
     7261 gtccacggtc gctgcgctga tgcacagttc ttccgcgatc tcgcggctgc ttttgtcgct
     7321 gcagaccagc accagtattt ccagctctct cggggaaagt tgcgccatgc cgctgcgaaa
     7381 ttgcggcacg cgtggttttt catcaatccg gtattccggt attctcgtcg aggagaccgt
     7441 gcttcagcgc gtatttcacg agcccgacgg tgttgcccac gccgagcttg atgagaatat
     7501 ttttccggtg cgtgtcgacc gtgccggtgc tgatgaataa ttttttcccg atctcggcgc
     7561 tggagaattc tttgcagaca agcacgagaa tctctctttc acgcgcggtg agcagcgtgc
     7621 cgggactgtc gtcgacgatg gcggaagatt tcaggcgcat attctcttcg tggtcgtagc
     7681 gaatgatgtc ttcgctgaaa tgttccccgc cgctcatcac tttccgcacg gcgtcgagca
     7741 gctcgtccag cgaaacgctt ttcagcaggt agccgcgtac gccgagtttg cggagcttgt
     7801 ccacgtaacg accgccgcgc tgcatggtga gcatgacgat cttcacagcg gaatggttcc
     7861 ggctcagcgc ttccgccact tcgatgccgt tcctgccggg aagatggagg tcgagcagga
     7921 gaaggtcggc cttcgttttt tccatcatcg cgatcgcttc gtcgccgtcc ttcgcggtcg
     7981 ccaccacttt cgcttcaccc gatttgttga gcaggatctc cagcccttcg aggtagagcg
     8041 ggtgatcatc ggcgagcgct atgcggtacg gcttctgcat gatcatctgt tcttcatcac
     8101 gatctgcgtc gtgtagcggc ttttcacttc gagcagcacc tgtttcgccg ttgtcacgcg
     8161 atcgatcgtt ggctgatcat aatagcggat ggtgacgagc tcgacgttct cgttgtacag
     8221 cacgcggaaa ttttccttca gccggtcgat gagcttcggc agcttttttt cgtcgctgtc
     8281 cacgcttacg gaaaagctga tcgcggaatt gagcatcacg tttattttca cacggaactc
     8341 cgcgaagtaa tcgaagatgt cgccgaggtt ttcttcgacg ataaaggaaa aatctttcgg
     8401 tgaaatggac agcagcacct ggttcacttt gaagatgaag cagggaatgg gaagcggaac
     8461 gtgcgcgtcg tggatctttg ttccttcttt cgcgggatcg aggaaggagc gcacgaagag
     8521 cggaatgttt ttgttctgca gcggcttgat cgttttcggg tgaatgaccg ttgcgccgta
     8581 atacgtcagc tcgatcgcat cctggtaagt gagccgggga atgagacggg tatcgtcgaa
     8641 ccatttcgga tcggcgttca gcacgcccgg aacgtctttc cagatcgtga cgctttccgc
     8701 atttgtgcac caggcgagga tagcggccgt ataatcggaa ccttcgcgac cgagcgtcgt
     8761 tgtgtaattt tcagaagtta cgccgaggaa tccctgcgtg acgacgatct ttcctttctt
     8821 ctccgtgaaa aaaggaacga gcgatgcgtc caccgctttt tccgtcaggt cccagtcgac
     8881 ttttccttcc cggtacgtgt tgtctgtttt gatgaaatcg cgcacgtcgc gccattgcgc
     8941 attcacaccg gcggagttga ggtaagcgct cacgatgcgc gtggaaagga tttctcccac
     9001 ggaaacgatc tggtcgtact cgcggtcgaa atcaccggac ggctcatctt cggtggccca
     9061 ttcgagttcc acgaacgtgt tgtggatctc gtcgtaaacg ggatgggttt tatcagggaa
     9121 aagttcgctg agaatggcga gatgaaaacc ttttatttcc gtgagaattt ttacgggatc
     9181 atctttctta aaaaagaaag cttccgccag cttctcgagc gcattggtcg tcttccccat
     9241 ggcggagata acgatgacga gattatcgga aggaaacagg cgaatgattt cagcgacatt
     9301 ttttacggct cctgcatctt tcaccgaagc cccgccgaac ttgaagattt tcatgagcac
     9361 gaaaatacga aagtcgcctt accggttact catgcaactg taagctcaaa gatgcgaagc
     9421 ccgtttcttc gcgccctgcg tgggagggtc cgtcgtactt cgtgattgat gtaaaaaacc
     9481 aggattattt ctcgaaatcg agattggccg gggaagatga aagcgggcgg gcaggagtga
     9541 tgtccgtttc cgtggtaagc ggaacggaat tgtccaccac ttcgaacgaa aaatcgtcga
     9601 accagatctg gccggttccg ccctgcagca tcgcgccgaa aacaatatcc gttgcgcccg
     9661 gcggaacgtc gaacacgatc tcgtatttcg tccatccggt cgttcctttc acggggcggt
     9721 cgcgcatgtt gtcgaagcag acaatgtttt ttccttccgg cgctgcctgg tcgacgcgca
     9781 gccagaggcc cgcgaagttg ctgacgtttt cggatttcat ccacgccgtc atgcgcacac
     9841 gcttgccgag gtactggtcg gccctgatga tctgaacgag tccgccgctg ccgcccggtt
     9901 ttgggacgag cgaacggatc gtggccgcgt tttttccatt gtgcgcagtc gcgtcaatgt
     9961 gcatgtcgta ttgataggcg gaatattcat acggttgcca tacgccgccg atgaaattct
    10021 catcttcagg aacaaaaccg atcggaccgg ggatagtttt ccatttattt ccgttgtagc
    10081 tggcgtgaaa ccccatgaca agatcacctc gctcaaccac attataaatc acttccaccg
    10141 aatcttttgc tggtttgaac tgcaatgaaa aatgcaggct gccgttccgc tcttttttcg
    10201 attttttcca gcggtaattg tattcaagaa gaataaagcc gcctttcata acgtatttcg
    10261 caatacgcaa tccggctgcg tcgtaaaaaa ccgctgttcc gtcggccagt ttcgatgatg
    10321 aagaaggatc cgttttcgtg taaatgactt tcgccgcatc ctgggggaaa acccatttgc
    10381 cgtaataaat gcaattcgat tttcctttgc tgttcaagag gtcgtagaac tcgtaaacgc
    10441 caccgtttgt tgttttctga aaagaagaat cgagttcgat tttcgcccag ccgggcgttt
    10501 tgccgagatc agtatcggtt aatacgggag tgtcctgcgc agcaaggcat gcaggcaata
    10561 cagcgatgac cgctgcgaag agaatgtttt tcatgatcgt actttggtgc tgttataacg
    10621 ggggaacggg ccaaagttac accggttatt cccggattct tgcatagtac ctcgtcacgc
    10681 aggcgttgta gcaggcccgg agggcgaggg cgctgcctgt tacaccaaga aaataaaaac
    10741 tcatggcatt ggcgttcccc aatggacggg tattagcgtt caaccatgga aatgcccgga
    10801 ccacagacaa gtttgcgccg gagtttcccg gttagcgcca tattcaggag ttgcggggca
    10861 ttccagccaa aagaccaccg aaattcacgc atgtatgtgt cgcagtttac ggaagatcat
    10921 ttcgccttgc ggtacattgt aaaaatggct aaatat
//
LOCUS       DASTSF010000148         3935 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_722490,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000148 DASTSF010000000
VERSION     DASTSF010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3935
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_722490"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="VFU15_04370"
     CDS             complement(<1..182)
                     /locus_tag="VFU15_04370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717039.1"
                     /translation="MHFSPGQISFAIFFIAVFAISLVWAYRRDKPTDRKYYVGVWKVL
                     ISLAIAIATVSFILKNL"
     gene            288..2405
                     /locus_tag="VFU15_04375"
     CDS             288..2405
                     /locus_tag="VFU15_04375"
                     /inference="COORDINATES: protein motif:HMM:NF026253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HEU4717040.1"
                     /translation="MFSRPGATFFFLVAFSVSLTAGRLEKGFRALQVYDYFKARALFI
                     SCSSHHPAGAGYGLAIIYSRNDNPFHNLPLAHANILCAQWNFPKTSAREKLRLFKLGI
                     SDSVIVSLKKSIDTLGFRAAEKTSTVDGWNIYLDEYCGSVLAQEATEKLHDLAWTLAQ
                     AANTYQAYKDFAETYPDAVQRPAALAGYERLLFETLTADSSISSYQNFIATYPQSPFI
                     SQAEDQLYALSVPHHSIPEYRDFIRKYPSNRNVPEAWRNIYALYTTDGKPTTIAQFWV
                     DYPDFPFKETISEDLRLSMTAFYPVREKDKWGFADSTGKIMIPCVYDWVGPFSQGIAA
                     AGRDGKSGYIGKNGKTVIPFNYDESEDFANGFAQVEQNGKTGLTDHAGHLVTPAVYDE
                     IGEFHEARAVVSRDGKYGYIDPTGNEVVPCIFEKAGDFSGGLAYISDSSKYGFVDRSG
                     KTVIRPQYEWAEPFTYGCARVEKDGKYGLIDFNGRVLVPCDYDFIADFSEGFALIVKD
                     DKCGYIRRNGSFAVPMQFDYDRTLLGESPFTGGKAKVLLDDKCGLIDTNGKVVVPREF
                     DDIQVFSGGLAAVKKKDKWGFIDEKVRLVIPYKYDYAWSFSEGLARVRKDGMTGYIDP
                     NGKEVIALQFSDATDCANGYLKVKNESGWGLMDKKGNWILPCHYDKIEFISALELRVE
                     RDDKFGYYHLGRCEFFWKEEGLN"
     gene            complement(2438..3691)
                     /locus_tag="VFU15_04380"
     CDS             complement(2438..3691)
                     /locus_tag="VFU15_04380"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEU4717041.1"
                     /translation="MLHAGTGDPPRMRGLLVTPADTAGTTDNPSDTSQFVSSVVDEDP
                     RDTATDDGDPTKKQPSTSAAVADSSDNSDGMEDMIGGDFYDDFDTTIVHADKFDALSF
                     DDTIYIPLNDPAHNPYCHPFNGRPTSSFGFRSYRYHFGVDIDLETGDSVKCAFDGKVR
                     IAQHSKSYGYVVVVRHNNGLETYYAHLSKLLVHPGEDVNAGTILGLGGNTGHSHGSHL
                     HFEVRFGGQPINPNYLIDFDKKQLRTDTYCLCKSDFKYLTETYRVRHYSRKKKKTWYT
                     YYCPGGARYSTPQAKAVMAKVPPPVMPGTTPKPFIAPVVSDSVASTTTKADNSVSKPA
                     HTTTASSPPAKKTTTAKPAAKKTSPPPKNSATASSGKVFYTVKKGDTLSGIAVRYGTS
                     VQKICDLNGIKSSTVLQIGRKLRVK"
BASE COUNT          987 a          963 c         1073 g          912 t
ORIGIN      
        1 aggttcttga ggatgaatga aacggtggct atcgcgatcg caagcgaaat aagcaccttc
       61 cagacaccca cgtaatattt gcggtccgtg ggtttgtcgc gccggtaagc ccacaccagg
      121 ctgatggcga aaacagcgat aaagaaaatg gcgaacgaga tctgtcccgg cgaaaaatgc
      181 atgcgcaaat ttagggagaa actaagcgca ggaagttttg acggtgaaca aaaaagcgcc
      241 ggaatgcgtt attcctgtac ccgcaggctt tattgtaaat ttggcagatg ttttcccgtc
      301 ccggcgcaac gttttttttc ctggtcgctt tctcggtttc cctgactgcg ggaaggcttg
      361 aaaaaggatt ccgggccctg caggtttacg attatttcaa agcgagagcg ctcttcattt
      421 cctgctcatc gcatcacccg gccggcgctg gctacggact cgcgatcatt tattcacgga
      481 acgacaatcc ttttcataac cttccgctcg cgcacgcgaa catcctgtgc gcgcagtgga
      541 atttccccaa aacatccgca cgcgaaaagc ttcgcctgtt taagctcggc atcagcgaca
      601 gcgttattgt ttcgctgaaa aaatcgatcg acacgcttgg gttccgtgca gcagaaaaaa
      661 cgtcaaccgt ggatggatgg aacatttacc tggacgaata ctgcggttcc gtcctggcgc
      721 aggaggcgac ggaaaagctg catgatcttg cgtggacgct ggcgcaggcg gcaaacactt
      781 accaggcgta taaggatttt gcagaaacct acccggatgc tgtccagcga ccggccgcgc
      841 tcgccggata cgaacgcctg cttttcgaaa cgcttaccgc cgacagcagc atttcctctt
      901 accagaattt cattgcgacg tatccgcaaa gtccgttcat ttcacaggca gaagaccagc
      961 tctacgcact ttctgttccg catcattcca ttcccgaata ccgtgatttt atccggaaat
     1021 atccgtccaa ccgcaatgtt cccgaagcct ggcggaatat ttatgcgttg tacaccaccg
     1081 acggaaaacc gaccaccatt gcacagttct gggtggatta cccggatttc cctttcaaag
     1141 aaacgatcag cgaagacctg cgcctttcca tgacggcttt ttatcctgtg cgcgaaaaag
     1201 acaaatgggg cttcgccgac agtacgggaa agatcatgat cccgtgcgta tacgattggg
     1261 tcggtccttt ctcgcaggga attgcagcag ccgggcgcga cgggaaatcg ggttacatcg
     1321 gcaagaacgg aaagaccgtc atcccgttca actatgacga atcggaagat ttcgcgaatg
     1381 gttttgcgca ggtcgagcag aacgggaaga ccggcctaac ggatcacgca ggacatcttg
     1441 taacgccggc tgtttacgat gagatcggcg aattccacga agcgcgcgcc gttgtttcgc
     1501 gtgacgggaa atacggctac atcgatccca ccggcaatga agtcgttccg tgcatctttg
     1561 aaaaagccgg agatttttcc ggcgggctcg cttacatttc cgacagttcg aaatacggtt
     1621 tcgttgaccg ctcaggcaaa accgtgatcc gtcctcaata cgaatgggcg gaaccgttca
     1681 cgtacgggtg cgcgcgcgtg gaaaaagacg gaaaatacgg cctgatcgat ttcaacggca
     1741 gggtgcttgt gccgtgcgac tatgatttca ttgccgattt cagcgaaggg ttcgcgctga
     1801 tcgtgaaaga tgataagtgc ggttacattc gccgcaacgg gtcgtttgcc gtgcccatgc
     1861 agttcgatta tgaccgcacg ctgctcgggg aaagcccgtt cacgggcggg aaagcgaaag
     1921 tgctgctgga tgacaaatgc ggtctcatcg acacgaacgg gaaagtggtt gttccgcggg
     1981 agttcgacga catacaggtt ttttcaggag gacttgcggc cgtgaagaaa aaagacaaat
     2041 ggggattcat tgacgaaaaa gtgaggctgg ttattcctta caagtatgat tatgcgtgga
     2101 gcttcagcga gggacttgcg cgtgtgcgga aagacgggat gacgggctac atcgatccga
     2161 acggcaagga agtgatcgcg ctccagtttt ccgacgccac ggattgcgcg aacggctacc
     2221 tgaaagtgaa aaacgaaagc ggctggggac tgatggacaa aaaaggcaac tggatacttc
     2281 cctgtcatta cgacaaaatc gaattcatca gcgcgcttga attacgggtg gaacgggatg
     2341 ataagttcgg ttactatcac ctcggccgct gcgagttttt ctggaaagag gaaggactga
     2401 actgaaaggg gaaacgaaac ccggctgaat tcccggatta tttcacacgg agtttgcggc
     2461 cgatctgcag gacagtggaa gatttgatac cgttgagatc acatatcttc tgaacagatg
     2521 tgccgtagcg gaccgcaatg ccggaaagcg tatcaccttt cttcaccgtg taaaagacct
     2581 tcccggaaga agcagtagcg gaatttttcg gcggtggcga agtctttttt gcagcgggtt
     2641 tggctgttgt cgttttcttc gccgggggag aagaagccgt cgtggtatgg gccggttttg
     2701 aaactgaatt atccgctttt gtggtcgttg aagcaaccga atcggaaacg acaggtgcaa
     2761 taaatggttt gggcgtagtt ccgggcatca ccggcggcgg caccttcgcc ataaccgctt
     2821 tggcctgcgg tgtagaatat cgcgctccac ccgggcaata atacgtgtac caggttttct
     2881 ttttcttcct ggaataatgg cggaccctgt atgtttccgt caggtatttg aagtccgatt
     2941 tgcagagaca atacgtatcc gtacgcagct gctttttatc aaaatcgatg aggtaattcg
     3001 gattgatggg ctgacctccg aaacgcactt caaaatgaag atggctgccg tgggaatgac
     3061 cggtatttcc gccgagaccg aggatcgtgc cggcattcac atcctcgccg ggatgtacga
     3121 gcaattttga aaggtgcgcg taataggttt caagaccgtt gttgtgacga accaccacga
     3181 catagccgta gcttttactg tgctgcgcga tcctcacttt tccatcgaac gcacacttta
     3241 ccgagtcgcc ggtttcgaga tcgatatcaa cgccgaaatg ataacggtaa gaacggaacc
     3301 cgaatgaaga cgtcgggcgc ccgttgaacg gatgacagta aggattatgg gcaggatcgt
     3361 tgaggggaat gtaaatcgta tcgtcgaatg aaagcgcgtc gaatttatca gcgtgaacga
     3421 tcgtcgtatc gaagtcgtcg taaaaatcac cgccgatcat atcttccatc ccgtcgctgt
     3481 tgtcgctgct gtcagcaaca gcggccgatg tcgagggctg cttttttgtc gggtctccgt
     3541 cgtctgtggc cgtgtccctg ggatcttcat caacaactga tgaaacgaac tgagatgtat
     3601 cggatggatt atccgtggtg cccgcagtat cagccggtgt cacgagcagg ccacgcatgc
     3661 ggggaggatc tcccgtacct gcgtgcagca tgtgtaccgc accgcaaaaa acgacgacag
     3721 aaaaaaggat atgttggagt tttctcaaac ctttgataat taaaagaata gcaggggttg
     3781 aaaaaagttg cgctaaaata accattatac cgagggaagc aaattttttt atcccacacg
     3841 gcattattga taactaatac tgctctaaga aacagcccga aaggcccgta aacattgggt
     3901 tctgcaggag aagccgctcc tgttaaaaaa gccca
//
LOCUS       DASTSF010000149         3338 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_579377,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000149 DASTSF010000000
VERSION     DASTSF010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3338
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_579377"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3338)
                     /locus_tag="VFU15_04385"
     CDS             complement(<1..>3338)
                     /locus_tag="VFU15_04385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717042.1"
                     /translation="PYTIQEATPGDWHYPLVDVNGTNYSYYLDADGTRSTVTISHPSA
                     GVYSPDVVNTALVYWNTTTNTYYTYPENLAHIEDFVAAWKPSWAQSLVQYHPEYCYYQ
                     TCHEYSRKQITSDNMTSDQFDNLLRGTNTFADAVSAGFIKSSYASFPDPNDRLEDWFT
                     LSTSHPYDPFVTNTSTFGNYSTVLQHTFDDYISYDTSTYSMLEAASMSVRCGLNYGVL
                     PGNSCMQFGSDYVTGGPTSVNDSIRDLEWNTFKSLYLSAKLRIQNDRSDSLALHDCNT
                     LNDCIGEGDDFNPINAGMLNFSWMSWSGSEYYNNGEPCSVYNFMLYAGKQKRFPDAEN
                     YQNISPSDAAYQQYLQTGQCPEARALQDLTGAIFASDSMTEDSVRLMSFMQYNGFYMA
                     KHDYAPQYPISTEAYWWNGSVDGDTLNVVWTNPNASGDTVCSMLFDKHLTSITNWTDI
                     VGFKDLQASAFDGTNYSFTALALLPPVVTGDPYSYQEISGSTSCVDILNCKFPVSCPP
                     SGFAKDIQLLMTALAANGDLSDSALNLETSTYLPFVTPRIRSQVGSPSDSLTWTLDTA
                     NDIFILRRINATSNWVELKVINATPSGVSYDSIESFANIMGEDHNSFEMDGIDSTGAT
                     IVHLELVADWLTSADTEAISMGTCGHPTPVACDEREHHVRTDLETLLASVLLQKPFTD
                     DTDLFRTTAMTALLRSYLPPSDSATDAVYVQDTVSGIHYDTLTYSIPGCHLQLAHSDT
                     LNPAMPLDSLIDLYGLTGYGTPDAQGNYYDFTLTAVYKIGSDTISDTLFGSSCWPLKN
                     CTACPTPPEEEEEESLSLFSPSGGSPTPDGGGLSCEGWYAVYIQTLKDYNDSDYAAWS
                     GVYLDTALYYDFPSFARAELCGCVESYIDYLQAYLADTADTTLAIPVDIDHYTGCRQN
                     VLALDDPCKDAYADYITAVYKYNTFVYENPGLGFDSLALEYSYTRFTSDSLCYCVGEF
                     VSLVNEITDGRYNDNPDTAQSMLHFANYCEAYLAVPCTNTAVLDTFVSPPASYSNPCV
                     EYKINTALANAQLAYDDYIDSLTNAIANEYRSHCMQAVENFTDTYTEKEYHFTLYYYD
                     QAGNLVKTVPPEGVEPLDIDSSGD"
BASE COUNT          760 a          823 c          979 g          776 t
ORIGIN      
        1 atcaccggaa gaatcgatgt cgagcggttc cactccttcc ggcggaacgg ttttcacgag
       61 gttgcccgcc tggtcatagt aatacaaggt gaaatgatat tctttttccg tgtaggtgtc
      121 cgtgaaattc tccacggcct gcatgcaatg cgaccggtat tcattggcaa ttgcattcgt
      181 aaggctgtcg atgtagtcgt cgtaagcgag ctgcgcgttc gccagggcgg tattgatctt
      241 atattccacg cagggattgc tgtacgaagc gggcggtgaa acaaacgtgt cgagcaccgc
      301 agtattggtg cacggcaccg caaggtaggc ttcgcagtaa ttggcaaaat gcagcatgct
      361 ctgcgccgta tcgggattgt cgttgtacct gccgtccgtt atctcattca ccagcgaaac
      421 gaattcgccc acgcaatagc agaggctgtc cgaagtgaaa cgcgtgtagg aatattccag
      481 cgcaagcgaa tcgaaaccga gaccgggatt ttcatagacg aacgtattgt atttataaac
      541 ggcggtgatg taatccgcgt acgcatcctt gcacggatcg tcgagtgcaa gcacgttctg
      601 ccggcagccg gtgtagtgat cgatgtcaac gggaatggcc agcgtcgtat ccgctgtatc
      661 ggccaggtac gcctgcaggt aatcgatgta cgattcgacg cacccgcaca attccgcgcg
      721 ggcgaaggac ggaaaatcat aatacaacgc ggtgtcgagg taaacgcccg accatgcggc
      781 gtaatccgaa tcattataat ctttcagtgt ctggatgtag accgcatacc atccttcgca
      841 actcagtcct cctccgtcag gcgtcggact tccgcccgaa ggtgaaaaaa ggctcaggga
      901 ttcttcttcc tcttcttccg gcggagtcgg gcacgcggta caattcttca atggccagca
      961 ggaagaaccg aaaagcgtat cgcttatggt atcgcttccg atcttgtaaa cggcagtcag
     1021 tgtaaaatca taatagttgc cctgcgcatc cggtgttccg taaccggtca gcccgtaaag
     1081 atcgatcagg ctgtcgagcg gcatggcagg attcagcgta tcgctgtgcg caagctgcag
     1141 gtgacagccg ggaattgaat aggtaagtgt gtcgtaatga attccgctta ccgtatcctg
     1201 cacgtaaacg gcgtccgtag ctgaatccga cggaggcagg taggaacgca gcagcgcggt
     1261 cattgccgtt gtgcggaaaa gatcggtgtc gtccgtaaat ggtttttgca gcaggacgga
     1321 ggcaagcagc gtttcaagat cggtacgcac gtgatgttcg cgttcgtcgc aggcgacagg
     1381 cgtgggatga ccgcacgttc ccatgctgat cgcttccgta tcggccgatg tcagccagtc
     1441 ggcgaccagc tcaaggtgaa cgatcgtggc accggtggaa tcgataccgt ccatttcgaa
     1501 cgaattatgg tcctcgccca tgatgtttgc gaacgattcg atggaatcgt agctcacacc
     1561 ggaaggtgtt gcgttgatga ccttcagctc gacccagttg ctggtggcgt ttatcctgcg
     1621 gagaatgaaa atatcgttcg ccgtgtcgag cgtccacgtc agtgaatccg acggacttcc
     1681 tacctggctg cgtatacgcg gagtgacaaa ggggagatac gtgcttgtct cgagattcag
     1741 cgcggaatcg gagagatctc cgttcgctgc gagcgccgtc atgagcagct ggatgtcttt
     1801 cgcaaaaccg gaaggcggac agctcacggg gaatttgcag ttgagaatat ccacgcagga
     1861 agtggagccg gaaatttcct ggtaggaata cggatcaccg gtaaccacgg gaggcaacag
     1921 cgcaagagcg gtgaaggaat aattcgttcc gtcgaacgcg gaagcctgca ggtctttgaa
     1981 cccgacgatg tcggtccagt tcgtgatcga cgtgagatgt ttatcgaaaa gcatcgagca
     2041 aaccgtatcg ccggatgcat tgggattcgt ccatacgaca ttcagcgtat ctccgtccac
     2101 cgatccgttc caccaatacg cttctgttga aatgggatat tgcggggcgt aatcgtgctt
     2161 cgccatgtaa aaaccattgt actgcatgaa gctcatcagc cgcacgctgt cttcggtcat
     2221 gctgtcggag gcgaagatcg cgcccgtaag atcctgcagg gcgcgtgctt ccgggcactg
     2281 gcccgtttgc aggtattgct ggtaagcggc gtcggaaggt gaaatgttct ggtaattttc
     2341 cgcatccggg aaacgcttct gcttcccggc atagagcata aaattgtaaa cgctgcaggg
     2401 ttcaccgttg ttgtaatatt ccgagccgga ccagctcatc caggaaaaat tcagcatacc
     2461 cgcgttgata gggttgaaat catctccttc gcctatgcaa tcgttcagcg tgttgcagtc
     2521 atgaagcgca agcgaatccg agcggtcatt ctggatgcgg agcttggcgg agaggtacag
     2581 cgacttgaac gtgttccatt ccaggtcgcg gatgctgtca ttcacggaag taggtccgcc
     2641 ggtcacgtaa tcacttccga actgcatgca gctgttgccg gggagcacgc cgtaattcag
     2701 tccgcagcgc acgctcatgg atgccgcttc gagcatggag tatgtgctgg tgtcgtatga
     2761 aatataatca tcgaaggtgt gctgaagaac ggtgctgtaa ttcccgaacg tggacgtgtt
     2821 ggtgacgaac ggatcatacg ggtggctcgt cgatagcgtg aaccagtctt ccaggcgatc
     2881 gttcggatcg gggaatgacg catacgaaga tttgataaag ccggcggaga cggcgtcggc
     2941 gaacgtgttc gttccgcgca ggagattatc gaactggtcg ctggtcatgt tatcggaagt
     3001 gatctgcttc cggctgtatt catgacatgt ctggtaatag cagtattcgg gatggtattg
     3061 taccagcgac tgcgcccagg aaggtttcca tgcggcgacg aagtcctcga tgtgcgcaag
     3121 attttcagga taggtatagt acgtattggt tgtcgtgttc cagtaaacca gcgcggtgtt
     3181 taccacatcc ggagaataaa cgcccgcgga cggatgcgaa atagtgaccg tggaacgcgt
     3241 gccgtctgca tcgaggtagt agctgtaatt cgttccgttc acatcaacga gggggtaatg
     3301 ccagtcgccg ggcgtggctt cctgtattgt gtaaggaa
//
LOCUS       DASTSF010000150         3212 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_828140,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000150 DASTSF010000000
VERSION     DASTSF010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3212
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_828140"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..244)
                     /locus_tag="VFU15_04390"
     CDS             complement(<1..244)
                     /locus_tag="VFU15_04390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717043.1"
                     /translation="MNQELVRKYRSGKLPAGIWLYSCLAGAVVAGVESVVLWDNSFNG
                     AWAGMFLRVFIGNLLYTLPVFFAQAHFVAIALQKKKG"
     gene            complement(252..785)
                     /locus_tag="VFU15_04395"
     CDS             complement(252..785)
                     /locus_tag="VFU15_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717044.1"
                     /translation="MELEPGTIIEHDHYGKGKVLENHELFVKVDFGKRGVMDVTKRNS
                     DLITVVSLPGEEPQYDFTMEELEKTLRRILDEYIAASPDVEMGGKWEGGTMILKPSDD
                     SLKPKEIPLETFFHKIVMLRDRLRVMEQKINAHPKLTDEEKVEMQQYITKIYGSLTTF
                     NVMFRHKEDYFEGESSK"
     gene            complement(810..2594)
                     /gene="argS"
                     /locus_tag="VFU15_04400"
     CDS             complement(810..2594)
                     /gene="argS"
                     /locus_tag="VFU15_04400"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013996716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEU4717045.1"
                     /translation="MQSLESLLSSSVSKAVESLFGIRAEASSVTFQRTNPQFEGDITL
                     VVFPYVKASRKGPEQTAQVIGEYLKANVPEVESFNVVKGFLNLVIGQSFWADVFASMN
                     ADENFGKKKAGEKAPLVVVEYSSPNTNKPLHLGHIRNNLLGLSVSEILKANGNEVKMV
                     NLVNDRGIHICKSMLAWLKFGNGETPESSGMKGDHLVGKYYVEFDKHNKAEIEKKVEQ
                     GHEREEAAKHTHLMFEAQEMLRKWEAGDEETIALWKKMNGWVYDGFAQTYKMLGVHFD
                     KTYYESDTYKLGKEIIDEGLKKNVLQKENGAVIIDNTPEGLDKKVLLRSDGTSVYMTQ
                     DLGTALERHKEFHFDRHIYVVGNEQDYHFKVLKISLKKLGYDWADGLYHLSYGMVELP
                     EGKMKSREGTVVDADDLIGEMEKSARELAEEKGLIEGMENDEKEKLSHVVGMAALKYF
                     ILKVDPKKKMLFNPKESIELTGNTGPFIQYTYARIRSMWRKSGMEKNPAPVISFPLHE
                     KEKELIKILYAFPAAVNEAAAGLSPAVIANYSYDLAKVFNQFYDVCPVLKEENGTVRD
                     FRIALALNVAKVVKTALGLLGIEVPERM"
     gene            complement(2598..>3212)
                     /locus_tag="VFU15_04405"
     CDS             complement(2598..>3212)
                     /locus_tag="VFU15_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015808136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HEU4717046.1"
                     /translation="YPKGRLGVIWIDAHADIHSPWTTPSGNLHGMPIAASLAEDNIAN
                     KMNKPDRETIELWKKLKEVGGISPKIQYKDLVYIGVRDVEPEETSLLKKHNVKMFTTS
                     EVKRNGVERIARDTLSHLSHCSHIYISFDVDSMDSSISKGTGTPVRNGITEKEAGSLL
                     VRLIQNEKVCCFEVCEVNPTLDKENLMAENTFEILTKVVAQLTN"
BASE COUNT          641 a          890 c          788 g          893 t
ORIGIN      
        1 ttcccttctt cttctgtaag gctatggcga caaaatgcgc ctgcgcaaaa aacacaggga
       61 gcgtataaag caggttgccg atgaaaacgc gaagaaacat cccggcccag gcaccgttga
      121 aagaattatc ccagagaaca acgctttcca cacctgcgac gactgcgcct gccaggcagg
      181 aatacagcca gatcccggcg ggcagttttc ccgaacggta tttccgtacc agctcttggt
      241 tcatggaagg atcattttga agattcgcct tcaaaataat cttctttgtg ccggaacatc
      301 acgttgaatg tcgtcaggct gccataaatt tttgtgatgt actgctgcat ctccactttt
      361 tcctcatcgg tgagcttcgg atgcgcgttg atcttctgct ccatcacgcg gagacggtcg
      421 cgcagcatga cgatcttgtg gaagaatgtc tccagcggaa tttccttcgg cttcagcgaa
      481 tcatcggagg gtttgaggat catcgttccg ccttcccatt ttccgcccat ttccacgtcg
      541 ggtgatgcgg cgatgtattc gtcgaggatg cggcgcaatg ttttttcaag ttcttccatg
      601 gtaaaatcgt attgaggttc ttcgcccgga agcgaaacga cggtaatcag gtcgctgttg
      661 cgcttcgtga cgtccatgac gccgcgcttc ccgaaatcca cttttacgaa aagctcgtgg
      721 ttttccagca cttttccttt gccgtagtgg tcgtgctcga tgatcgttcc gggttccagc
      781 tccattgacg ctaagataaa ccgtaaattc tacatgcgtt ccggcacctc gatgccgaga
      841 agtccaagcg cagtcttcac caccttcgcc acattcagcg caagcgcaat gcggaaatca
      901 cgcacggtcc cgttctcttc cttcagcacg ggacatacat catagaactg gttgaagact
      961 ttcgcgaggt cgtaggaata gttcgcgatc acggccgggc tcagtcctgc tgccgcctca
     1021 ttcacggctg cgggaaacgc gtaaagaatt ttgatcagct ctttttcttt ttcatgcagc
     1081 gggaaagaaa tgaccggcgc aggatttttt tccatgccgg acttccgcca catgctgcgg
     1141 atacgcgcgt acgtgtactg gatgaacgga cccgtgttgc ccgtaagctc gatgctttcc
     1201 ttcgggttga agagcatttt ctttttcggg tcgactttga gaatgaaata tttcagcgcg
     1261 gccatgccca ccacgtgcga aagcttttcc ttttcatcgt tctccattcc ttcgatcagt
     1321 cctttctctt ccgccagctc gcgcgcgctt ttttccattt cgccgatgag gtcgtccgca
     1381 tccaccaccg ttccttcccg cgatttcatt tttccttcgg gcaattccac catgccgtac
     1441 gacagatgat agaggccgtc cgcccaatcg taaccgagct ttttcagcga gatcttcagc
     1501 accttgaaat ggtaatcctg ctcgttgccg acgacgtaaa tgtgccggtc gaaatgaaat
     1561 tccttgtgac gctcgagcgc cgtgccgagg tcctgcgtca tgtacacgga tgttccgtcg
     1621 ctgcgcagca gcactttttt atcgagtccc tccggcgtat tatcgatgat caccgccccg
     1681 ttctctttct gcagcacatt tttcttcagg ccttcgtcga tgatctcctt tcccaatttg
     1741 taggtatccg attcgtaata cgttttatcg aaatgcacgc cgagcatttt gtacgtctgc
     1801 gcaaaaccgt cgtacaccca gccgttcatt tttttccaga gcgcgatcgt ttcttcatcg
     1861 cctgcttccc atttgcgcag catttcctgg gcttcgaaca tcaggtgcgt atgcttcgcc
     1921 gcttcttccc gctcgtgccc ctgctccact tttttttcga tctctgcttt attgtgctta
     1981 tcaaactcca cataatattt tccgacgagg tgatcgccct tcatgccgga tgattccggc
     2041 gtttccccgt taccgaactt cagccacgcc agcatgcttt tgcagatgtg aataccgcgg
     2101 tcgttgacga ggttcaccat cttcacttca ttgccgttcg ctttcagtat ctcggaaacc
     2161 gaaagcccga gcaggttgtt gcggatgtgc ccgaggtgaa gcggcttgtt cgtattgggc
     2221 gaactgtatt ccaccaccac gagcggcgct ttttcgcccg cttttttctt cccgaaattt
     2281 tcatcggcgt tcatcgaggc gaaaacatcc gcccagaagc tttgcccgat cacgaggttg
     2341 agaaagcctt tcaccacgtt gaagctttcc acttcgggaa cgttcgcttt gaggtattcg
     2401 ccgatcacct gcgcggtctg ttcgggtcct ttgcgcgacg ctttgacata aggaaaaacg
     2461 accagcgtga tgtcgccttc gaactgcggg ttcgtgcgct ggaacgtaac ggaagaagct
     2521 tccgcccgga taccgaatag tgattcaacg gctttcgata cgctggagga aagaagggat
     2581 tcgagggatt gcataaatca gttcgtgagt tgggcgacca ctttggtcag tatttcaaat
     2641 gtattctcgg ccatcaggtt ttccttatcg agggtgggat tcacttcaca cacttcgaag
     2701 cagcaaacct tttcgttctg gatcaggcgc accagcagcg atccggcttc tttctccgta
     2761 atgccattgc gcacaggtgt gccggttccc ttgctgatgg acgaatccat gctgtccacg
     2821 tcgaatgaaa tgtagatgtg ggaacaatgg ctcaggtggc tcagcgtgtc gcgggcaatg
     2881 cgctcgacgc cgttcctttt cacctcggaa gtggtgaaca tcttgacatt atgcttcttc
     2941 aggagagatg tttcttccgg ttccacatca cggacaccga tgtagacaag gtctttgtac
     3001 tggatcttgg ggctgatgcc gcccacctct ttcagctttt tccacagttc tatcgtttcc
     3061 cggtcgggtt tgttcatctt attcgcgatg ttgtcttcgg caagtgaagc cgctatcggc
     3121 atgccgtgaa ggttaccgga aggcgtcgtc cagggtgaat ggatatcggc gtgggcatcg
     3181 atccagatga cgccaagtct tcctttcggg ta
//
LOCUS       DASTSF010000151         3525 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_947536,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000151 DASTSF010000000
VERSION     DASTSF010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3525
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_947536"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(442..807)
                     /locus_tag="VFU15_04410"
     CDS             complement(442..807)
                     /locus_tag="VFU15_04410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717047.1"
                     /translation="MTRFRWRRFFFVTVAALLLPVFVYSQTDGGGGGNSNAGSGAGNN
                     DATKTPDSRAQRKKAKKDWKKQRKQQIDDQKAINQYNKTYNTKKTRKRMKKDKKKAQQ
                     NNEHKRDFFIKRWFHRKRY"
     gene            881..1297
                     /locus_tag="VFU15_04415"
     CDS             881..1297
                     /locus_tag="VFU15_04415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717048.1"
                     /translation="MRRFVFLLVFLLCCAGCKKEHQYIPNVYVNLTIYVNDPGNIRLT
                     TVGGWKYFDGGNRGIVVYRKSQNEFVAYDRTCPYKPDESSSVVSVDTTNNILLKDASC
                     TSQFLMSDGTPIGGPAVIPLKLYRSIFDGTTLHVTN"
     gene            complement(1345..2664)
                     /gene="ahcY"
                     /locus_tag="VFU15_04420"
     CDS             complement(1345..2664)
                     /gene="ahcY"
                     /locus_tag="VFU15_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010609317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HEU4717049.1"
                     /translation="MSKAITEMRLPYKVKDISLAEWGRKEIKLAEAEMPGLMALRAEY
                     GASKPLKGARIAGCLHMTIQTAVLIETLVELGAEVTWSSCNIFSTQDHAAAAIAAAGI
                     PVYAWKGMNAEEFDWCIEQTLFFGEERKPLNMILDDGGDLTNMVFDKYPELAAGIKGL
                     SEETTTGVHRLYERMNKGTLMVPAINVNDSVTKSKFDNKYGCRESLVDAIRRATDLML
                     AGKVAVVAGYGDVGKGSAQSLSSQGVRVIVTEIDPICALQAAMDGYEVKKMDDAVKRA
                     DIVVTATGNKDIVVERHFRAMKHNAIVCNIGHFDTEIDMAWLNKNYGKTKDVIKPQVD
                     KYTIEGKDIIVLAEGRLVNLGCATGHPSFVMSNSFTNQTLAQIELWNHSDKYEKKVYT
                     LPKHLDEKVARLHLAKIGVELDTLSKDQADYIGVKVEGPFKPEHYRY"
     gene            2764..3384
                     /locus_tag="VFU15_04425"
     CDS             2764..3384
                     /locus_tag="VFU15_04425"
                     /inference="COORDINATES: protein motif:HMM:NF013786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HEU4717050.1"
                     /translation="MPLVKRIEAGKNSFAGIWEVTETLEDLQGKSGGTEKIPEEFIRE
                     ERKRQWIAVRALLEALRPGKKIVYDTNGKPFTDDPQTNISISHSGIYVLVYVSDELCG
                     ADIERVSGKAERIAKRFLSEREFTEAAQLPAGALITYWAAKEAAYKREGVKEISFRSD
                     IFVGKPDDAEMGIVPVQIYRGGKSINLRVRYETFNDYRIALTEPAA"
BASE COUNT          841 a          850 c          931 g          903 t
ORIGIN      
        1 aagaacaacg taaagttttc gcagcataat gattctgttt cgtgttgttg tgtataggtc
       61 gtgcgatttg aagtcggtaa atatagagag ttttttttgt gaagggcaaa tttactttta
      121 acaaaaattt acatgtcaaa agcacaaaat ctgccatgga aaacacgacg aaaaacacgc
      181 taaataaccg ttgaaatcaa taaaaaacct cctgccgagg gatgttgcag gcggcgcagg
      241 aaatattggt gaattttcct gcgtgaactc cgtgtgtctt tttacggaag ttctggggaa
      301 ttacagtaaa gaggtcgccg aagcggaaag aacgattgaa tcaaatttag agattctgtt
      361 aatacctgca aaaatccatt cgtgtattct ttcgaccggc tgcccggaaa aggcagatgg
      421 cggctcctgc aggataagtg gtcagtacct tttgcggtga aaccagcgtt tgatgaagaa
      481 atcgcgctta tgctcgttgt tctgctgcgc ctttttcttg tccttcttca tccgcttcct
      541 ggttttcttc gtgttgtacg tcttgttgta ttggttgatc gccttctggt cgtcgatctg
      601 ctgcttgcgc tgtttcttcc agtccttttt cgcttttttc cgctgggccc tgctgtcggg
      661 cgttttcgtg gcgtcattgt ttccggcgcc cgaacccgca ttgctgtttc cgccaccacc
      721 gccgtcggtc tgggaataaa caaaaacggg cagcagcagg gcggcaaccg tcacgaagaa
      781 aaaccgtctc catctaaagc gcgtcattgt acaaatctac ttaattccca aacgcacaaa
      841 aactgttccg attgtgaagc aaccttcctt aaatttgtcc atgcgccggt tcgttttcct
      901 gttggttttc ctgctttgct gtgcagggtg taaaaaagag caccagtaca ttccgaacgt
      961 ttacgtcaat ctcacgattt atgtgaacga tccgggaaac atacggctga ccacggtggg
     1021 cggatggaaa tatttcgacg gcgggaaccg tggcatcgtg gtgtaccgga aatcgcagaa
     1081 tgaatttgtg gcttatgacc ggacctgtcc gtacaagccg gacgagtcct catccgtggt
     1141 ttccgttgat acaaccaaca atatcctgct gaaggatgcc agctgcactt cgcagttcct
     1201 gatgagcgac ggcactccca tcggcggacc tgcagttatc ccgctcaagc tgtaccgttc
     1261 gatttttgat ggaacaacgc ttcacgtaac caattaaaaa aggcccaatg cagggccttt
     1321 tcaacggaaa tccttcccgg tttatcaata gcgataatgt tcaggcttga aggggccttc
     1381 cactttcacg ccgatatagt cggcctggtc tttggaaagc gtatccagtt caacgccgat
     1441 cttggccagg tggaggcgcg ctactttttc atcgaggtgc ttcggaagcg tgtatacttt
     1501 cttctcgtat ttgtcggaat gattccagag ttcgatctgc gcgagcgtct ggttggtaaa
     1561 tgaattcgac ataacgaaag aaggatggcc cgttgcgcaa ccgagattca cgaggcgacc
     1621 ttcggcgaga acgatgatgt ctttgccttc gatcgtgtat ttgtccacct gcggtttgat
     1681 aacatctttc gttttcccgt aattcttatt cagccatgcc atgtcgatct cggtgtcgaa
     1741 gtggccgata ttacaaacga tggcattgtg cttcatcgcg cggaagtggc gttcaaccac
     1801 gatgtccttg ttcccggttg cggtgaccac aatgtccgca cgctttaccg catcatccat
     1861 tttcttcact tcgtaaccgt ccatggccgc ctgcagtgcg cagatcggat cgatctcggt
     1921 aacgatcacg cggacgccct gtgagctgag cgactgcgcg gatccttttc cgacatcgcc
     1981 atagccggcg acaaccgcga cttttccggc gagcatgaga tctgtagcgc ggcggatcgc
     2041 atcgacgagg gattcgcggc aaccgtattt gttgtcgaat ttcgatttcg taaccgaatc
     2101 attcacattg atggcgggaa ccataagcgt tcccttgttc atgcgttcgt aaagacggtg
     2161 aacgccggtg gttgtttctt cggaaagacc ttttatacca gcggcaagct ccgggtattt
     2221 atcgaacacc atattggtaa ggtcgccgcc gtcatcgagg atcatgttga gcggcttgcg
     2281 ttcctcaccg aagaaaagcg tctgctcgat gcaccagtcg aattcttcgg cattcattcc
     2341 tttccatgcg tagacgggaa taccagccgc ggcaattgct gccgctgcgt gatcctgcgt
     2401 ggagaaaata ttgcaggacg accaggtaac ctcggcgccc agctcaacga gcgtttcgat
     2461 aagaacggcc gtctggatgg tcatgtgcag gcagccggcg atgcgggcgc ctttgagcgg
     2521 ttttgacgcg ccgtattctg cgcggagcgc cataaggccg ggcatttcag cctcagccag
     2581 cttgatttct tttcgtcccc attctgcgag ggaaatatct tttaccttgt acggaagtcg
     2641 catttctgtt atggctttgc tcattgctgg ttattttgga ctgcaaattt aggaaaaagg
     2701 cggtaaagca gaggtaaaga gttgcgcagc gcgggtaaaa gttttcgata ttgcgagaca
     2761 attatgccat tggtaaaaag gatcgaagcc gggaagaatt cattcgcagg aatctgggag
     2821 gtgacggaaa cgctggaaga cctgcagggg aaaagcggcg gaacggagaa gattccggaa
     2881 gaattcatac gcgaggagcg taagcggcaa tggattgcag taagagcatt gcttgaagcg
     2941 ctgaggcccg gaaaaaaaat agtgtacgac accaacggaa aaccgttcac ggatgatccg
     3001 cagaccaaca tttccatttc gcattccggg atttacgtcc tggtttacgt ttccgatgaa
     3061 ctttgcggag cggatatcga gcgggtatcg gggaaagcgg agcgtatcgc aaagaggttc
     3121 ctcagcgaaa gagagttcac cgaagccgca cagctgccgg ccggcgcact gatcacttac
     3181 tgggccgcga aggaagcggc ctacaagcgt gaaggcgtaa aagaaatatc gtttcgttcg
     3241 gatatttttg tcggaaagcc ggacgacgca gaaatgggaa ttgtacctgt gcagatttac
     3301 cgcggtggaa aaagcatcaa cctgcgcgtt cggtacgaaa cgttcaatga ttaccgcata
     3361 gctttaacag aaccagccgc atgaaaaata tccgggaaaa gctcgccagg gaaaccgctt
     3421 caggccggaa gcagctggct gtgcttgtcg atccggataa gacagcgcat cttgaaacgc
     3481 tcctggaagc agcagaaaaa tttccgccgg gctattttct tgtcg
//
LOCUS       DASTSF010000152        12092 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1617957,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000152 DASTSF010000000
VERSION     DASTSF010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 12092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12092
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1617957"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1437
                     /locus_tag="VFU15_04430"
     CDS             <1..1437
                     /locus_tag="VFU15_04430"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEU4717051.1"
                     /translation="ASGDEDLMLMSADLNGTQQWIKTYGGTGSDYGYDVKKTFSGGYV
                     LAGTTGSFSSAGADADNAFLAATDANGNILWSDDFGGMNADGFFSVAQAPDSGFVAAG
                     FTSSFGQGGKDVFVVRTDKNGNTTWMKSFGAAGDDIGYSLCITSSSDILVSGTTSSFP
                     AGYRVLLLKIDLNGNTEWSKYYSYSYNATTLSESGNCVIQNAAGNYVIGGRLGLDFIG
                     AAQPFLMCTDTSGNNISWCNYYMLNSGPSRLGSVEETSPGGGYIIGASMSGGLLSMIR
                     VSSGGGASWANYYSTASYSIGGKGYSATHTSDGGFILTGVFTAQPDSAVALIKTDAAG
                     NAGCNTYAPLGGGGTNSQTVTPSDAGFASASGGVSFPVTVTVASPALSATVLCSTAGI
                     DDKNETGNIGISPNPSCGSFTVDIPADFPPGQQLSLCDAAGRTVLVSWLKNGKNFIRT
                     ADLAPGVYFYSITSPDGETTARGKMVSE"
     gene            complement(1447..2490)
                     /locus_tag="VFU15_04435"
     CDS             complement(1447..2490)
                     /locus_tag="VFU15_04435"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF021535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEU4717052.1"
                     /translation="MRFRFARAVLTDLIFIPLFCFCIFNLPLVAYGLKMAKGELTIVW
                     NARPVKDVLADPAVPDSVKAKLKLIEEIRQFAIDSIGLSPSKNYTTFFNQQGKRLIYV
                     VTACRPFAMQPHLWHFPVLGDVPYKGFFEKEKAMDEENRLKKEGWDTDIGGASGWSTL
                     GWFKDPVLSQMLAYREGDLAELIIHELTHGTIFVKDSVEYNENLASFIGYKGALWFLE
                     SKYGKDSKQYTDYVNGRKDDETLEKFMLACAHSLDSLYKSMPAGMDSLQKVSLKKNAF
                     RKFAENSRQLPFAADTLFPKRFAKRIEKSGNTFFMQYVRYGAKQVNFENEFARFANLR
                     AYMKWLKKKYGKE"
     gene            2703..3464
                     /locus_tag="VFU15_04440"
     CDS             2703..3464
                     /locus_tag="VFU15_04440"
                     /inference="COORDINATES: protein motif:HMM:NF014724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEU4717053.1"
                     /translation="MFFLLSKLLSFLIMPLTWIFLLLFFSFRGKDDRRKKRYRIAAVV
                     LILLFTNRFIFDRVMHVWEINTIQTPPKGSYDAIIILGGISTYDPDFDRVQFSNGNDR
                     LMQGLLLLKEGVAPKILFTGGSGSVLHSDKKEGVWIRKFLRTYGIPDSCMIFENESRN
                     THENALFSKPLLAKYAPGGRYLIVTSGFHMRRALGCFSKAGIRAIPFSADRYSGPLKF
                     EFDYAFIPDAETLSCWNTLFHEWFGCVAYKLSGYM"
     gene            3548..3952
                     /locus_tag="VFU15_04445"
     CDS             3548..3952
                     /locus_tag="VFU15_04445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717054.1"
                     /translation="MSKDHPGQETKTVIAGQVEIWLSEGIVRVSYLDGADITKEIKMQ
                     MHHEFLRITGGKKYPFLFRADGSLWYTREGREYARHIESLQPFLAVAMVAPSLGFRLL
                     AEFYGKFYKPEKPYKVFKTDEEAETWLRSFMN"
     gene            complement(3956..5695)
                     /locus_tag="VFU15_04450"
     CDS             complement(3956..5695)
                     /locus_tag="VFU15_04450"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEU4717055.1"
                     /translation="MKKIPFLLIVIIAASCGAAGIHFTVHNPKKAGKYPKRTQAIELM
                     GNQESKYRTCFDATYYKLDVSFGKNIETDHSISGTVAMNAVCVSDFDTLQIDLAKNMN
                     CISIHKADISSSGETPEDELKFYRREGAMFVLFPKTVHKGETFGLIISYNGTPLEAKR
                     PPWAGGFVWKKDDLGNPWDGVACETEGASLWWPCKDVVNDEPDSADMSFTVPDNLVAV
                     SNGRLVSRAHADEAAGMKTFDWHVSYPINLYDITFYIGKFKLLHDIYVSPVTHDTLQL
                     NHYVLEQHYDKAKTQFAQLKDYLAFYEQTYGPYPWYRDGFKLVESPYEGMEHQTAIAY
                     GNGFKNDEQWGFDYIILHETAHEWWGNSVTAYDLGDGWLHEGFATYTEALWVEHKFGH
                     DAYERYLLFDRLFIGNERPVSRPYNIRYFDYHDEDIYMKGTWVLHSLRYVLNDDSLFF
                     GILHSFYGKYKYSNATSKDFEDLVNEKTGKDYTWFFRQYLYNRFTPELHYCLKDGYLY
                     YKWEKTADDFVMPAKVEVYGHIGTWLDVNATAVSVGRIPYSGERAGVFNDRKFLFKPV
                     EDVAVARLYGKSG"
     gene            5903..6325
                     /locus_tag="VFU15_04455"
     CDS             5903..6325
                     /locus_tag="VFU15_04455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717056.1"
                     /translation="MQNFLLDARWDIPCKNDWEGMRMPGGGFPFLSTYDCNEYCGTSG
                     GMYTVGWNLVLNCVIMFAASFLLWYFFRKKLEQLLASRFSWIYVVWVVFISAPSLLFL
                     FFALAFNSDNSWIWNSQPPFDGCTPHMHLYLFMLFRVF"
     gene            complement(6346..6498)
                     /locus_tag="VFU15_04460"
     CDS             complement(6346..6498)
                     /locus_tag="VFU15_04460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717057.1"
                     /translation="MSADGAIVKTMAVPGNTQEIDTSDLAPGVCEFIVTDESGRMFRS
                     TWVKTG"
     gene            complement(6510..6674)
                     /locus_tag="VFU15_04465"
     CDS             complement(6510..6674)
                     /locus_tag="VFU15_04465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717058.1"
                     /translation="MKKSLFPALLCLSVWLHAQPVLFVYNRCYGGTGNEDRCFDAMEM
                     QPGTLLTVKN"
     gene            complement(6733..9387)
                     /locus_tag="VFU15_04470"
     CDS             complement(6733..9387)
                     /locus_tag="VFU15_04470"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013632564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEU4717059.1"
                     /translation="MEIPKTYDPKSTEDKWYGWWMEKGFFHSVPDEREPYTVVIPPPN
                     VTGVLHMGHMLNNTIQDVLVRRARMQGKNACWVPGTDHASIATEAKVVALLREKGISK
                     KDLSREKFLEYAWEWKEKYGGIILSQLKKLGASCDWERTRFTMEEELSESVIDVFIDL
                     YRKGYVYRGTRMVNWDPSALTAVSDEEVIMKESTSKLFYVRYKIEGTTDEWVTIATTR
                     PETILGDTAVCVHPEDERYAKLKGKRVIVPLVNRSVPVIFDEYVDKTFGTGALKVTPA
                     HDRNDYELGKKHKLESIDVLNPDGTMSKAAQLYVGTDRFACRRQIVKDLEEKGHLVKT
                     EELKNNVGYSERTNAVIEPRLSMQWFVAMKEISKPALEHVLNGDVKLIPEKFINTYKY
                     WMENVQDWCISRQLWWGQRIPAWYDADGNFVVAKTAEEAVVQFETVHGKSYTANQVRQ
                     DEDVVDTWFSSWLWPISVFDPAVFAKGKEYKGNADLQYYYPTNDLVTAPEILFFWVAR
                     MIIAGCEYRGEKPFTNVYLTGTVRDKLGRKMSKSLGNSPDPLELIEKYGADGVRVGML
                     LCSPAGNDIMFDESLCEQGRNFSNKIWNAFRLVKSWSVDDSVAQTDAAKVAVEWFDAR
                     FNQQLAEINDHYAKFRLSDALMATYKLVWDDFCAWYLEMVKPEFINGKASPVDGKTYL
                     ATVGFFENILKVLHPFMPFITEELYHHLEDRDLKDAIIVARWPEERAYDSGVIQRFSA
                     FAESVMGVRTIRNEKNISPKEPLELYIRIPDNPVSRRLEGAIKKLCNISAIHYTDKPV
                     DNASSFICAGIEFYVPLPKNFDLAAEKEKLKKELDYNKGFLVSVQKKLANEKFVQNAK
                     PELVENERRKEADAVAKIKALEAQLANL"
     gene            9534..10127
                     /locus_tag="VFU15_04475"
     CDS             9534..10127
                     /locus_tag="VFU15_04475"
                     /inference="COORDINATES: protein motif:HMM:NF019242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HEU4717060.1"
                     /translation="MKKLLLIPIFLVGVATLAKAQAPTTSGGQDNKDGPVMTFDVTEY
                     NFGTIKQGDIVTYEFKFKNTGKEPLIINNAVGSCGCTVPEWPKEPIRANGSGVIKVTF
                     NSAGKLGQQDKTVTLTYDTDKQIQLHLKGTVEAPAATPAPGANTPAAPAQTGGGKTAT
                     ATPAASTSPATKSSGTNAPAGSKSGSTNAPAGDKPKN"
     gene            10146..10682
                     /locus_tag="VFU15_04480"
     CDS             10146..10682
                     /locus_tag="VFU15_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HEU4717061.1"
                     /translation="MKKIVALSVLCFGMVCAYAQTPATKTATKPATKPAATATTTTTA
                     PASGTVADAGYSFDKMVYDYGTIDKGADPYCEFQLTNNSKEPLVIQEAHGSCGCTVPE
                     YPKDPIKPGQTVTIRVRYDTNRVGPFDKSVTITFQGKDQPAVLKIHGVVKAPPADTPF
                     PGPGQNNSNSGAPVNGGN"
     gene            10702..11127
                     /locus_tag="VFU15_04485"
     CDS             10702..11127
                     /locus_tag="VFU15_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013996847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HEU4717062.1"
                     /translation="MKKILSLFLFLAVVSFAGAHPSSAYVSFDGGGPVITFDSETIDF
                     GTLVQGNEAIRTFTFTNTGDQPLVISSIKGQCGCTTFPDSWPKDPVPPGGKASFRVKY
                     DTAVRVGMFDKKIMVYCNATNAVNGVFEVKIKGNVQAAQ"
     gene            11165..>12092
                     /locus_tag="VFU15_04490"
     CDS             11165..>12092
                     /locus_tag="VFU15_04490"
                     /inference="COORDINATES: protein motif:HMM:NF019242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HEU4717063.1"
                     /translation="MQVRTLFIHHLVAQYLHDDGIVRKTLSLAFSFLSFFLCAQSRDT
                     ANAPVITFDETTHDFGTVVQGTVVTHEFRFTNTGKTPLVITNVCGSAGSVVPSFTKEP
                     VAPGKNGMVKVIFSTTGNLGMQDKTVTLTSNAKDNTVVLHIKGQVIFPEQPLLVEKVI
                     LRNALQKPVDEYGKPLNGQSQQWPGCFLDIDTVTTGEKISFAFILRNQGNSPVRIMDV
                     TNDKYISSNDEYISASASKISVAPGDTVKISATLSTKRLGPERRSVIVSYFPDDTRTY
                     TSLYLHVKGFVIDPSLPVLSLSENGPLHDTVTQ"
BASE COUNT         2768 a         3346 c         3029 g         2949 t
ORIGIN      
        1 gcttccgggg atgaagatct catgctgatg tcggccgacc ttaacggaac gcagcaatgg
       61 ataaaaacat acggtggcac cggcagcgat tacggctatg acgtgaaaaa aactttttcc
      121 ggtggttatg tgcttgcggg aactaccggg agcttctctt ccgccggagc ggatgccgat
      181 aatgctttcc tcgccgcgac ggatgcgaac ggaaatattc tctggtcgga tgatttcgga
      241 ggaatgaatg cggacggttt tttttcagtg gcccaggcgc ctgacagcgg tttcgtcgct
      301 gcgggattca cgtcgagctt cggacaggga ggaaaagatg tttttgtcgt acgcaccgac
      361 aagaacggca acaccacgtg gatgaaatcg ttcggggctg ccggggacga catcggctat
      421 tcgctgtgca tcacttcttc ttcagacatt ctcgttagcg gaaccacgag cagcttcccg
      481 gccggttacc gcgtcctgct gctgaagatc gatctgaacg gaaataccga atggtcgaaa
      541 tattattctt attcgtacaa cgccaccacc ctgagcgagt ccgggaattg cgtcatacaa
      601 aatgcagccg ggaattacgt gatcggcggt cgtctcggtc ttgattttat cggcgccgca
      661 caacctttcc tcatgtgcac cgacacttcg ggcaacaaca tcagctggtg caattactac
      721 atgctgaatt ccggtccgag caggctgggt tccgtggaag agacctctcc cggcggagga
      781 tacatcatcg gggcctccat gtcgggcgga ctcctcagca tgatccgcgt ttcatccggc
      841 ggaggagcaa gctgggcaaa ttactattcg accgcttcgt attcgatcgg gggaaaaggt
      901 tattccgcca cgcatacttc cgacggagga tttattctca cgggcgtttt caccgcgcag
      961 ccggattctg cggtagcgct gataaaaacg gatgcagcgg gaaatgcagg atgcaacact
     1021 tacgctcctc tgggcggtgg cggaaccaat tcacaaacag tcacaccttc cgatgcggga
     1081 tttgcgtccg ccagcggcgg tgtttcattc ccggtaaccg taacggttgc ctctcccgct
     1141 ctttcagcaa cggtattgtg cagcaccgcg gggatcgatg acaagaatga aaccgggaac
     1201 atcggcattt cacccaatcc atcctgcgga tcattcaccg ttgacattcc ggcggacttt
     1261 cctcccggtc agcagctctc cctttgtgat gctgcgggaa gaactgttct tgtttcatgg
     1321 ctgaaaaacg ggaagaactt catacgcacc gcggatcttg ctccgggagt ttatttttat
     1381 tccattacca gcccggacgg agaaacaacg gcccgcggaa agatggtttc ggaataagat
     1441 cgcagcttat tctttcccgt acttcttctt cagccacttc atgtacgcgc gaaggttcgc
     1501 aaaacgtgca aattcatttt cgaaattcac ctgtttcgcg ccgtaacgga cgtattgcat
     1561 gaagaacgtg ttccccgatt tctctatgcg ctttgcaaat cgtttcggga acaacgtatc
     1621 tgcagcgaac gggagctgcc tggaattttc cgcgaatttc cggaacgcgt ttttcttcag
     1681 tgaaactttc tgcagcgaat ccatcccggc gggcatgctt ttgtacaggc tgtcgagtga
     1741 atgcgcacat gcgagcatga atttctccag tgtttcatcg tcttttctcc cgttgacgta
     1801 atcggtgtat tgcttcgagt cttttccgta tttgctttcg agaaaccata acgcgccttt
     1861 gtagccgatg aagctggcga ggttctcgtt gtactccacg ctgtctttca cgaaaatggt
     1921 tccgtgcgtg agttcgtgga tgatcagttc ggcaagatcg ccttccctgt acgcgagcat
     1981 ctgcgaaaga acaggatctt tgaaccagcc gagcgtagac catcccgatg cgccgccgat
     2041 gtccgtatcc cagccctctt ttttcagccg gttctcctcg tccatcgctt tttccttttc
     2101 aaaaaaacct ttgtacggca catcaccgag caccggaaaa tgccagagat gcggttgcat
     2161 ggcgaagggt ctgcacgccg tcacgacgta gatgaggcgc ttcccctgct ggttgaaaaa
     2221 cgtcgtgtaa tttttgcttg gtgaaagccc gatgctgtcg atggcgaact gccgtatttc
     2281 ttctatgagc ttcagcttcg ccttcacgga atccggcact gcaggatcgg cgaggacgtc
     2341 cttcaccggt cgcgcgttcc acacgatcgt caattcccct ttcgccattt tcagtccgta
     2401 cgccaccagc ggaagattga aaatgcagaa acagaaaagc gggatgaaaa tgaggtcggt
     2461 taaaacggcg cgggcaaagc ggaaacgcat ggatcaaagg tagctaagta cagaattagt
     2521 cggagagggt cacaagctcc aaatccacaa caacaaacaa ccctggttca acgtctgaaa
     2581 attcaaagtt gaaaaactgt tccgggtttc aaaatcagaa caacaaacgg ttacctacct
     2641 ccggaaaact gagctgaaaa accgtcaccc gggactaaga agcaatactt acatttacat
     2701 ccatgttctt cctcctcagc aagctgctct cgttcctgat catgccgttg acgtggatct
     2761 tcctgcttct ctttttttca ttcaggggaa aagacgaccg ccggaaaaaa cgttaccgga
     2821 tcgccgctgt ggttcttatt cttctcttca ccaaccgctt cattttcgac cgcgtaatgc
     2881 acgtgtggga gatcaacacg atacagacgc cgccgaaggg aagctacgac gcgatcatta
     2941 ttctcggcgg catcagcacg tacgacccgg atttcgaccg tgtgcagttc tccaacggga
     3001 acgaccgtct catgcaggga ttgctgttgc tgaaagaagg cgtcgcaccg aaaattcttt
     3061 tcaccggcgg ttccggcagc gtattgcaca gcgacaaaaa agaaggcgtg tggatacgca
     3121 aattcctccg gacctacggt attcccgatt cgtgcatgat cttcgagaat gaatcgcgga
     3181 acacgcacga gaacgcgctt ttctccaaac cgctgctcgc caaatacgca cccggcggac
     3241 gttaccttat tgttacttcg gggtttcaca tgcgccgtgc gctgggctgc ttcagcaaag
     3301 ccggtattcg cgccattcct ttctccgccg accggtacag cggcccgctg aagttcgaat
     3361 tcgactatgc gttcattccc gacgctgaaa cgctgtcgtg ctggaatacg cttttccacg
     3421 agtggttcgg ctgcgtggcg tacaagctgt ccgggtatat gtaaccacgg ttattatttt
     3481 tttcgcagcg gacttttaat aaccgcgatt attattatct tcaccgcatc ccgcgaaaaa
     3541 attaagtgtg tcaaaggatc accccggtca ggaaacgaaa acggtgattg caggccaggt
     3601 tgagatctgg ctgagcgaag ggatcgtgcg cgtcagttat ctcgacggcg ccgacatcac
     3661 caaagagatc aaaatgcaga tgcaccatga atttctccgc atcaccgggg gaaaaaagta
     3721 cccgttcctt ttccgcgcgg acgggtcgct ctggtacacg cgcgagggcc gggaatatgc
     3781 gcgccacatc gaatcattgc agccttttct cgccgtcgcc atggtggctc ccagcctcgg
     3841 ctttcgcctg ctcgcggaat tttacgggaa attttacaag ccggaaaagc cgtacaaggt
     3901 tttcaaaacc gacgaagaag cggaaacctg gctgcgctcg ttcatgaatt gaacctcagc
     3961 ctgacttgcc atacagccgc gccaccgcga cgtcctccac cggcttgaac aggaacttac
     4021 ggtcgttgaa cacgcccgcc cgctcaccgg aatacgggat ccttcccacg gaaacagccg
     4081 tcgcatttac atccagccac gtcccgatgt gaccgtacac ttccactttt gccggcatca
     4141 caaaatcatc cgctgtcttt tcccatttgt aatacaggta gccgtccttc aggcagtaat
     4201 gcagttccgg cgtaaaacgg ttgtagaggt actggcgaaa aaaccacgtg taatcttttc
     4261 ccgttttttc attgacgaga tcttcaaaat ccttcgacgt ggcattgctg tatttgtatt
     4321 ttccgtagaa cgaatgcagg ataccgaaaa aaagcgaatc gtcattgagc acataacgca
     4381 atgaatgcag cacccatgtt cccttcatgt aaatgtcctc gtcgtggtaa tcgaaataac
     4441 ggatgttgta cgggcgtgaa accgggcgtt cgttaccgat gaaaagacgg tcgaaaagca
     4501 ggtaacgctc gtaggcatcg tgcccgaatt tatgctccac ccacagggct tccgtgtacg
     4561 tggcgaatcc ttcgtgcagc catccgtcgc cgagatcata cgccgtcacg gaatttcccc
     4621 accactcgtg cgccgtctca tgcaggatga tgtagtcgaa gccccactgc tcgtcgttct
     4681 tgaacccgtt cccgtaagcg atcgcggtct ggtgctccat tccttcatac ggcgattcga
     4741 ccagcttgaa cccgtcgcgg taccatgggt acggaccgta ggtctgctcg taaaatgcga
     4801 ggtaatcctt cagctgcgcg aactgcgttt tcgccttgtc ataatgttgc tcgagaacgt
     4861 aatgattgag ctgcagcgta tcgtgcgtga cgggtgaaac gtaaatatca tgcagcaatt
     4921 tgaatttccc gatataaaat gtgatgtcgt aaagattgat cgggtaggaa acgtgccagt
     4981 cgaatgtttt cattcccgct gcttcatctg cgtgcgcgcg gctgaccagt cttccgttcg
     5041 aaaccgcgac aagattatcc ggaacggtga atgacatatc cgcgctgtcg ggttcgtcgt
     5101 tcaccacgtc tttgcacggc caccacaggc tcgcaccttc cgtttcgcag gccacgccgt
     5161 cccacgggtt cccgagatcg tctttcttcc agacgaaccc gccggcccat ggcggacgtt
     5221 ttgcttcgag cggcgttccg ttatagctga tgatgagccc gaacgtttct cctttgtgaa
     5281 cggttttggg gaacagcacg aacatggcgc cttccctgcg gtagaatttc agttcgtctt
     5341 ccggtgtttc acctgaagaa gaaatatccg ccttgtgaat ggaaatacag ttcatgttct
     5401 tcgcgagatc gatctgcagc gtatcgaaat ccgaaacgca tacagcgttc atcgcaacgg
     5461 tcccggaaat gctgtgatcg gtctcaatgt tcttcccgaa gctgacatcg agcttgtaat
     5521 acgtcgcgtc gaagcacgtc ctgtatttcg attcctggtt tcccatcagc tcgatcgcct
     5581 gcgttctctt cgggtacttt cccgcttttt tcgggttgtg aacggtgaag tgaatgccgg
     5641 ccgctccgca ggatgcagcg atgatgacaa tgaggagaaa gggtattttt ttcatgcagc
     5701 taaatttaaa ggaattcacg tcccgaaaat ttccattcat cctggcggtt tttaagctcc
     5761 atgagctgcc cgtttcagag atgatttttc acctcctgct gcggatacct tcctgatcgc
     5821 ttaacttgca tacatcaact ccgcaccgca cctgaaaaaa aacctgcgcg tcttttttgt
     5881 cttcatcctc ccgcttgccc tcatgcagaa tttcctgctc gacgcgcgct gggatattcc
     5941 ctgcaaaaac gactgggagg gaatgcgcat gccgggcggc gggttcccgt tcctcagcac
     6001 gtatgactgc aatgaatact gcggcacgag cgggggaatg tatacggtgg gctggaacct
     6061 cgtgctgaac tgcgtcatca tgttcgccgc gtcattcctg ctctggtatt ttttccggaa
     6121 aaagctggaa cagttgctcg cctcccgctt cagctggatc tacgtggtat gggtcgtttt
     6181 catttctgcg ccttccctgc tgtttttatt cttcgcgctc gcgttcaatt ccgacaactc
     6241 gtggatatgg aattctcagc cgcctttcga cgggtgcacg ccgcacatgc atctttacct
     6301 cttcatgctt ttccgggtct tctgaaatag ttccgccgga aattcctatc cggtcttcac
     6361 ccacgtcgaa cggaacatcc gcccgctctc atcggttacg atgaattcac agacgccggg
     6421 cgccaggtcc gacgtatcga tttcctgcgt gtttcccggc acagccatgg ttttcacgat
     6481 cgcaccatct gcgctcatca cgcgaagaat taattcttta cggtaagcag cgttccgggt
     6541 tgcatttcca tcgcgtcgaa gcaacggtct tcattaccgg ttccgccgta gcaacggtta
     6601 taaacgaaca gaacaggttg cgcatgcagc cacacggaca ggcacagcaa agcaggaaaa
     6661 agactttttt tcacgagggc cgggattaat gaatgaaaaa caaaaacgga aataaaatcc
     6721 gcgatgctca gtttaaaggt tcgccagctg cgcttccagc gctttgatct tagccacggc
     6781 gtccgcttct ttcctgcgct cattctccac cagctccggc tttgcgttct gcacgaattt
     6841 ttcgttggcc aatttcttct ggactgaaac gagaaaacct ttgttgtaat cgagctcttt
     6901 tttcagcttt tccttttcag cagcgagatc gaaattcttc ggcagcggaa cataaaattc
     6961 aattcccgcg cagatgaacg acgacgcgtt gtccacgggt ttgtccgtgt aatgaatggc
     7021 ggaaatattg cacagctttt tgatcgcgcc ttccagcctg cggctcaccg ggttgtccgg
     7081 gatgcggatg tacagctcga gcggctcttt cggggaaatg ttcttttcgt tgcggatcgt
     7141 acgtacgccc atgacggatt ccgcaaatgc gctgaaacgc tggataacgc cgctgtcgta
     7201 ggcgcgttct tccggccatc tggcaacaat gatcgcgtct ttcagatcgc ggtcttccag
     7261 gtgatggtac agctcttccg tgatgaacgg catgaacggg tgcagcacct tgaggatgtt
     7321 ctcgaaaaac ccgaccgtgg cgaggtacgt tttcccgtcg acggggctgg ctttcccgtt
     7381 aatgaactcc ggcttcacca tttcgaggta ccacgcgcag aagtcatccc acacaagctt
     7441 gtacgttgcc atcagcgcat ccgacaaacg gaattttgcg taatgatcgt tgatctccgc
     7501 gagctgctgg ttgaaacggg cgtcaaacca ttccacggcc actttcgccg catcggtctg
     7561 cgcaacggag tcgtccacgc tccagctttt cacgaggcgg aacgcattcc agatcttgtt
     7621 cgagaaattc cttccctgct cgcacagcga ttcatcgaac atgatgtcgt tgcccgcggg
     7681 tgagcacaaa agcatgccca cgcgcacgcc gtcggcgccg tatttttcga tcagctccag
     7741 cggatcaggt gaattcccga gcgacttcga catttttctt ccgagcttgt cgcgcaccgt
     7801 acccgtgagg taaacgttcg tgaacggctt ttcgcccctg tattcgcagc cggcaatgat
     7861 catgcgcgcc acccagaaaa ataaaatttc cggtgcggtg acgaggtcgt tcgtcgggta
     7921 ataatactgc agatcggcgt tgcctttgta ttcttttcct ttcgcgaaaa cggcaggatc
     7981 aaaaaccgag atcggccaga gccaggaaga gaaccacgtg tccaccacgt cttcgtcctg
     8041 gcgtacctgg tttgccgtat acgatttgcc atgtacggtt tcaaattgca caacggcttc
     8101 ttcagcggtt tttgcaacga cgaaatttcc atcggcatcg taccaggcgg gaatgcgttg
     8161 cccccaccac agctggcgcg agatgcacca gtcctggacg ttctccatcc agtatttata
     8221 cgtgttgatg aatttttcag gaatgagctt tacgtccccg ttcagcacgt gttcgagagc
     8281 gggttttgaa atttccttca tcgccacgaa ccactgcatg gaaaggcgcg gctcgatcac
     8341 tgcgttcgtg cgctccgaat agccgacatt gtttttcagc tcttcggtct tcacgagatg
     8401 tcccttttct tcgagatcct tcacgatctg cctgcggcag gcgaaacgat ccgtgccaac
     8461 gtaaagctgc gccgctttgc tcatcgtgcc gtcgggattg agcacgtcta tgctttcgag
     8521 cttgtgcttt ttcccgagct catagtcgtt ccggtcgtgc gcgggcgtga ctttcagcgc
     8581 gccggtgccg aacgttttat ctacgtattc gtcgaagatc acgggaacag aacggttgac
     8641 gagcggaacg ataacccgct ttcctttgag cttcgcgtaa cgttcatctt cgggatgaac
     8701 gcaaacagct gtatcaccga ggatggtctc cggccgcgtg gtcgcgatcg tcacccattc
     8761 atcggtggtt ccttctattt tataacgaac gtaaaagagc ttcgaggtcg actctttcat
     8821 gatcacttcc tcatccgaaa cggccgtcag cgccgaagga tcccagttga ccatgcgcgt
     8881 gccgcggtac acgtagcctt tccggtagag gtcgatgaaa acgtcgataa cggattccga
     8941 caattcctct tccatcgtga agcgcgtgcg ttcccagtcg cacgaggcgc cgagcttttt
     9001 cagctggctg aggatgatgc cgccgtattt ttctttccat tcccaggcgt attccaggaa
     9061 tttctcgcgg gaaaggtcct tcttgctgat ccctttttcc ctgagcaggg caacgacctt
     9121 ggcttcggtg gcaatggacg cgtggtccgt tcccggcacc cagcaggcgt tttttccctg
     9181 catgcgggcg cggcggacaa gcacgtcctg gatcgtatta ttgagcatgt gccccatgtg
     9241 caggacaccg gtcacgttgg gcggaggaat gacaactgta tacggctcac gctcatccgg
     9301 aaccgaatga aaaaatcctt tctccatcca ccacccgtac catttgtctt cggtcgattt
     9361 cgggtcgtat gttttgggta tctccatact atactttatc ttcgtttccc ttgattttca
     9421 gggaatgtaa aactacaaaa aatgcccggc gtttattggc actgtggcac gattttggca
     9481 gcatattcac caggaaaaca ccttaaaaat aaaagtcaaa accaatcaca gctatgaaaa
     9541 aactgcttct tatcccgatt tttcttgtag gcgttgcaac gcttgctaaa gcacaggccc
     9601 ccaccacatc cggcggacag gacaataaag atggtccggt catgaccttc gatgttactg
     9661 aatacaactt cggcaccatc aagcagggtg acatcgttac ctacgaattc aagttcaaga
     9721 acaccggcaa agagccgctg atcatcaaca acgcagttgg ttcatgcgga tgcacggttc
     9781 ctgaatggcc gaaagaaccg atccgtgcaa acggaagcgg cgtgatcaaa gtaacgttca
     9841 actctgccgg caagctcgga cagcaggaca aaaccgttac gctgacttac gacacggaca
     9901 agcagatcca gctgcacctc aaaggaacgg ttgaagctcc ggctgcaacg cctgctcccg
     9961 gtgcaaatac gcctgcagct cctgcacaga caggtggcgg aaaaacggca acggctacgc
    10021 ctgctgccag cacttctccg gctaccaaat ccagcggcac caatgctccc gccggaagca
    10081 aatcaggcag cacgaatgct cctgccggcg acaagcccaa aaactaatct gaatttctgc
    10141 aaccaatgaa aaagatagtt gcactctctg tactttgttt cgggatggtc tgcgcgtatg
    10201 cgcagacccc cgcgacaaaa accgccacca aaccggctac aaaaccggcg gcgacggcaa
    10261 cgactacaac aactgcacct gcatccggca ccgtagctga tgcgggttac tcttttgaca
    10321 agatggtgta cgattacggc accatcgaca agggggcgga tccgtactgc gaattccagc
    10381 tgacgaacaa cagcaaagag ccgctcgtca tccaggaagc gcatggctca tgcggctgca
    10441 cggttcccga atacccgaaa gatccgatca agcccgggca aaccgttacg atccgcgtac
    10501 gctacgacac caaccgtgta gggccgttcg acaaatccgt gacgatcacg ttccagggga
    10561 aagatcaacc ggccgttctc aaaattcacg gggtcgttaa agctccgccg gcagatacgc
    10621 cgttcccggg tccgggacag aacaacagca acagcggcgc tccggtcaac gggggaaact
    10681 gatctctaac tttcaagcgc aatgaaaaaa atcctgtctt tatttctttt ccttgcggtc
    10741 gtttcgtttg caggcgcaca tccttcgtcg gcttacgttt cttttgacgg cggcggtcct
    10801 gtgatcacgt tcgattccga aaccatcgac ttcgggacgc tcgtgcaggg caacgaagcc
    10861 atccgcacat ttacattcac caacacgggc gatcagccgc tggttatttc cagcatcaag
    10921 ggacaatgcg gctgcaccac gttccccgac agctggccga aagatcctgt tcctcccggt
    10981 ggaaaagctt ccttccgcgt gaaatacgac acggctgtgc gcgtcggcat gttcgacaaa
    11041 aagatcatgg tctactgcaa cgcaacaaat gccgtcaacg gcgttttcga agtgaagatc
    11101 aaaggcaatg tgcaggcagc gcaatagctg catttttaat tacggttaat actgaagagt
    11161 ccgcatgcaa gtgcggactc ttttcattca ccatcttgtg gcgcagtatt tgcacgatga
    11221 cggtatcgtg cgcaaaacgc tgtccctcgc attttctttt ctttcttttt tcctgtgcgc
    11281 ccaatcccgg gacacagcga atgcacctgt gatcacattt gatgagacca cgcacgattt
    11341 cggaacggtt gtgcagggaa cggttgtaac gcatgaattt cgtttcacga acacgggaaa
    11401 aacgccgctg gtcatcacca atgtgtgcgg atcggcaggg tccgtcgtcc cgtccttcac
    11461 gaaagaaccg gtcgccccgg ggaaaaacgg aatggtgaaa gtcatttttt caaccacggg
    11521 gaatttggga atgcaggaca aaacggtaac tcttacctcg aatgcgaaag acaacacggt
    11581 tgtgcttcat ataaaagggc aggtcatctt tcccgagcag ccgcttctcg tggaaaaagt
    11641 catactcaga aatgcacttc aaaagccggt ggacgaatat ggaaaaccgc tgaacggaca
    11701 gtcgcagcaa tggcccggat gcttccttga tattgatacg gttaccaccg gggaaaaaat
    11761 cagtttcgca ttcatcctgc gcaaccaggg aaattcaccg gtgcggatca tggatgtgac
    11821 aaacgataaa tacatttcct caaatgatga atacatttcc gcatctgcat caaagatttc
    11881 cgttgcaccc ggcgacacgg taaaaatttc cgccaccctg agcacaaaac gtctcggccc
    11941 cgaaaggaga tcggtgatcg tgagttattt tcccgacgat acgcggacgt acacatcgct
    12001 ttacctgcat gtgaagggat tcgtcattga tccttccctt cctgttctct cgctttcgga
    12061 aaacggacct cttcacgata cggttacgca gg
//
LOCUS       DASTSF010000153         5133 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1985336,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000153 DASTSF010000000
VERSION     DASTSF010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5133
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1985336"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1955)
                     /locus_tag="VFU15_04495"
     CDS             complement(<1..1955)
                     /locus_tag="VFU15_04495"
                     /inference="COORDINATES: protein motif:HMM:NF014869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M4 family metallopeptidase"
                     /protein_id="HEU4717064.1"
                     /translation="MKKNILPLALAFVFVSMTGFAFAQQNFHGKLEGATIVSYSKYTG
                     LPNFVRFRPGTVETQSDFPSWLQKSLALGASVSFRPYSTEQDGIGFTHVRYKEYVGDF
                     PVENTMIIAHIKDDKVVSFNGDYYQDINPALSVSLSEQNALQFALNGVHAAKYKWENK
                     DEENAMKTMTHDPSFTYFPKGELVAVHQDGSDYSASSIHLTWKFNVYAEEPLSRNDIF
                     VDAQTGAIVHKRNLIENVDVPGSANTVYSGTQPITCDNFNTNQYRLRETGRGNGIETY
                     NLNNSTNYTNTDFTNNSSTWNLSPPDQAATDAHWGAEMTYDYYMNVHNRNSIDDAGFA
                     LLSYVHYSNNYVNAFWDGQVMTYGDGNVSQGYTIMTGLDVCGHEISHGLTTFTANLNG
                     GEADALNEGNSDIFGTTIEFYARPSNADWLIGADITTNGQGLRNMSDPKQLQQPDTYL
                     GQYWDQFGEPHNNNGPFIYWYYLLCQGGSGTNDNNDNYNVTGITMQEAQFIEYRGLSV
                     YFTPSTAYADARTYTIQAAQDLYGACSPEVISTTNAWYAVGVGAQFSASVAASFSADV
                     TNSCTLPATVNFTNTSSNASNATWNFGDNTTSTQTNPTHTYTQNGTYSVSLSVNSACG
                     TDSILQTNYITVNAPTTPVAAADTVSA"
     gene            complement(2081..2494)
                     /locus_tag="VFU15_04500"
     CDS             complement(2081..2494)
                     /locus_tag="VFU15_04500"
                     /inference="COORDINATES: protein motif:HMM:NF025442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4258 domain-containing protein"
                     /protein_id="HEU4717065.1"
                     /translation="MTWGRRIRLYLVGFGIGCLIVWFMFFRNSKRNLAGWLPSDRVTA
                     FIASSHKLNVDSALFCRMKCNGISIDDVRRSVMKGQVDFDRSDPHKEPCHEYDVKMNV
                     KGKDLEFYFSACVDDSTASILKIYPPLPGDSCGCN"
     gene            complement(2658..3878)
                     /locus_tag="VFU15_04505"
     CDS             complement(2658..3878)
                     /locus_tag="VFU15_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017843409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HEU4717066.1"
                     /translation="MKTSRDVLMSLTKGALMPQEEMLEVARKKGALSIGIPKEISFQE
                     NRVTLVPDDVGLLVNHGHRVIVETNAGKNANFQDNDYSEAGAEIAYSPSEVYKQAELI
                     LKVAPPSMDEIDFMQPKQILLSTLQLPAQPENFIKKLLNKKVTALAFEWIKDEDGVFP
                     VIRSMGEIAGGTSILIAAEYLSNVNNGQGAILGGISGVAPTEVIIIGAGTVGEYAARA
                     ALGLGATVKVFDNSVYRLRRLQSSLGTRIFTSIIQPKVLAKHLRTADVAIGALRASGG
                     RTPLVVNDTMVSEMKVGSVIVDVSIDQGGCFETSKVTNHTNPVFRKHGVIHYCVPNIA
                     SRVARTASYALSTIFAPIILNIGEEGGLTNYLRQTVGVRSGVYIYNGILTNQYLGEIF
                     NLPSKDIDLLVAAL"
     gene            4058..4429
                     /locus_tag="VFU15_04510"
     CDS             4058..4429
                     /locus_tag="VFU15_04510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717067.1"
                     /translation="MKFTRLTPNIFYTDIRVGLKLFVDCLEFEIGHDDLQNPEKPLCV
                     AGRDGLAVFLIQDKEYAEKDRPELRLETKNIDEVYAHVKSKFPELLHPNMKEVTLRPW
                     GARELGLLDESGVCVVVQQWG"
     gene            complement(4864..>5133)
                     /gene="tsaE"
                     /locus_tag="VFU15_04515"
     CDS             complement(4864..>5133)
                     /gene="tsaE"
                     /locus_tag="VFU15_04515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEU4717068.1"
                     /translation="LGVKEAMSSPTFSLVNEYRDAQDDPVYHFDLYRVKTVQEALDIG
                     LEDYLYSGNYCFIEWPEIIENVLPENAVKVDISESNRGREIRVSG"
BASE COUNT         1214 a         1303 c         1460 g         1156 t
ORIGIN      
        1 caggcagaaa ccgtatcggc tgcggcaacc ggcgtcgttg gtgcattcac ggtaatataa
       61 ttcgtctgga gaatggaatc cgttccgcag gcgctgttca cgctgagcga cacgctgtat
      121 gttccgttct gcgtgtaggt gtgcgtggga ttcgtctgtg tgctcgtcgt gttgtcgccg
      181 aaattccacg tggcgtttga cgcattcgag ctggtattcg tgaaattcac ggtagccgga
      241 agcgtgcagg agttcgtcac atcggcagag aatgaagcgg cgacacttgc ggagaattgc
      301 gcgcccacgc ccaccgcgta ccatgcattc gtggttgaaa tgacttccgg ggaacatgca
      361 ccgtacagat cctgcgctgc ctgtatcgtg tacgtacgcg catcggcata agccgtgctc
      421 ggtgtaaaat aaacggaaag cccgcggtac tcgatgaact gcgcttcctg catggtgatg
      481 ccggtaacgt tgtaattgtc gttgttgtcg ttcgttccgc tgccgccctg gcaaagcagg
      541 tagtaccagt aaatgaacgg tccgttgttg ttatggggct caccgaactg gtcccagtat
      601 tgtcccaggt acgtatccgg ctgctgcagc tgtttaggat cggacatatt gcgaagacct
      661 tgtccgttcg ttgtaatatc cgcaccgatc agccagtcgg cattggaagg acgcgcgtag
      721 aattcgatcg tcgttccgaa aatatcgctg ttgccttcgt tgagcgcatc ggcttcgccg
      781 ccgttgagat ttgccgtgaa cgtggtaagt ccgtgcgaaa tttcgtgccc gcatacgtcg
      841 aggccggtca tgatggtata gccctgcgaa acgttcccgt cgccgtaagt catcacctgc
      901 ccgtcccaga aagcgttcac gtaattgttg ctgtaatgca cgtagctcag cagcgcaaat
      961 cccgcatcgt cgatgctgtt gcggttatgc acgttcatgt aatagtcgta cgtcatttcc
     1021 gctccccagt gcgcatcggt tgcggcctgg tcgggaggag aaagattcca cgtgctggaa
     1081 ttgttcgtga aatccgtgtt ggtgtaattg gtcgaattgt tcaggttgta cgtttcaata
     1141 ccgttcccgc gtcctgtttc gcgcaggcga tactggttgg tgttgaaatt gtcgcaggtt
     1201 atcggctgcg ttccgctgta gacggtattc gcagacccgg gcacatcgac attttcaatg
     1261 aggttgcgct tatgcacgat cgcacccgtc tgcgcatcca cgaagatgtc gttgcgcgaa
     1321 agaggctctt ccgcgtacac gttgaacttc cacgtgaggt gaatggacga cgcggaataa
     1381 tcgctgccgt cctggtgaac agcgacgagc tcgcctttcg ggaaataggt gaacgaagga
     1441 tcgtgcgtca tggtcttcat ggcgttctcc tcgtccttgt tttcccattt gtactttgct
     1501 gcgtgcacgc cgttcagcgc gaactgcagc gcattctgct cggaaagcga aacggaaagc
     1561 gcgggattga tgtcctggta ataatcgccg ttaaaagaaa cgaccttgtc gtccttgatg
     1621 tgcgcgatga tcatcgtgtt ctcgacgggg aaatcgccga cgtattcttt gtagcgtacg
     1681 tgcgtgaagc cgatgccgtc ctgctccgtg ctgtagggac ggaacgaaac ggaagcgccg
     1741 agcgcaaggg atttctgaag ccaggaagga aagtcgcttt gggtttcaac cgttcccggc
     1801 cggaacctta caaagttcgg cagtcctgtg tacttgctgt agctgacgat ggttgcgcct
     1861 tccagctttc cgtgaaaatt ctgctgggca aatgcgaagc cggtcattga tacgaagaca
     1921 aatgccagcg ccagcgggag tatgtttttt ttcatgcaga aatggtgtgt tgtaaaaaaa
     1981 tttgttggtt caacgggccg gaacgtgtgg caggaaattt tcccggaagc atgaaagtag
     2041 gaaaaacccg gccggtttca aacccggctt acgtcaaaaa tcagttgcag ccgcagctgt
     2101 cgccgggaag cggcgggtag atcttgagga tggaggcggt ggaatcatca acgcaggcgg
     2161 aaaaatagaa ttcgagatcc tttcccttca cattcatttt cacatcgtac tcgtggcagg
     2221 gttccttgtg cggatcgctg cggtcgaaat cgacctggcc tttcatgacc gacctgcgca
     2281 cgtcgtcaat ggaaatgccg ttgcacttca tgcggcagaa aagcgcggag tcgacattca
     2341 gcttgtgcga ggacgcgatg aacgccgtga cgcgatcgga cggaagccag ccggcgaggt
     2401 tgcgtttgct gttgcggaag aacataaacc acacgatgag gcagccgatg ccgaaaccga
     2461 cgagatacaa acggattctt ctgccccagg tcatggtgat taatgattat gaagtaaaga
     2521 tttcattgcc gggacgggca gggatagaag cggaaagccc ggaaccgcgg tgaggacttg
     2581 cagcggatag cccggcgccg tgacagcatg ctgttcacgg cggcccgccg ggaaaataaa
     2641 aatgcaatgc aggaacatca taacgcagct acgaggagat cgatgtcctt cgagggaagg
     2701 ttgaagatct cgccgaggta ttgattggtg aggattccgt tgtagatgta cacgccgctg
     2761 cgcacgccca cggtctgccg caggtaattg gtgagcccgc cttcttcgcc gatgttcagg
     2821 atgatgggtg cgaagatggt gctgagcgcg tacgaggctg tgcgcgccac gcgcgacgcg
     2881 atgttgggga cgcaatagtg aatgacgccg tgcttgcgga aaacgggatt cgtgtggttg
     2941 gtgactttcg acgtttcgaa gcagccgccc tggtcgatgc tgacgtcgac gatgacggaa
     3001 ccgactttca tctcgctcac catggtgtcg ttgacgacga gcggtgtgcg cccgccgctg
     3061 gcacgcagcg cgccaatggc aacatcggct gtgcgcaggt gcttcgcgag cactttcggc
     3121 tggatgatgg aggtgaagat gcgcgtgccg agggaactct gcaggcggcg aagacggtaa
     3181 acggaattgt cgaacacttt cacggttgca ccgaggccga gcgcagcgcg tgcggcgtat
     3241 tcgcccacgg ttcccgcgcc gatgatgatg acttccgtgg gagcgacacc gctgatgccg
     3301 ccgaggatgg cgccctgccc gttattgacg ttggaaaggt attccgccgc aataagaatg
     3361 gaagtaccgc cggcgatctc gcccatggag cggatgaccg ggaaaacgcc gtcctcgtcc
     3421 ttgatccatt cgaacgcaag cgcagtgact tttttattga gcagcttttt gatgaagttc
     3481 tcgggctgcg ccgggagctg cagcgtggaa agcaggatct gcttgggctg catgaaatcg
     3541 atctcgtcca tggaaggcgg cgccactttg aggatcagct ccgcctgctt gtacacctcc
     3601 gagggtgaat aggcaatttc cgcgcctgct tcggagtaat cgttatcctg gaaattcgcg
     3661 ttcttcccgg cattcgtttc cacgatcacg cggtggccgt ggttgacaag cagcccgaca
     3721 tcgtccggaa caagcgtgac acggttttcc tggaaggaga tctctttggg aatgccaatg
     3781 ctgagcgcgc cttttttccg cgccacctcg agcatttcct cctgtggcat cagcgcgcct
     3841 ttggtcagcg acatcagcac atcgcgggaa gtcttcatac gttttttccg gaaattttgg
     3901 tgtgcgtctt cttttacgga agagcgaaca taaagatacg acaaacaggg gacatgaaaa
     3961 ccgggcggcc ggcaacccgg atcgtgtttg acgaaacgac gcgtctgcgt atgtttgtcc
     4021 gtccgccccg aagtttcaat cctttacttc tcaccgcatg aaattcacca ggctcacacc
     4081 gaacatcttt tacaccgaca tcagggtcgg actgaaactt ttcgtcgact gtcttgaatt
     4141 cgagatcggg cacgacgatc tgcagaaccc ggaaaaaccg ctgtgcgtgg cgggaaggga
     4201 cgggcttgcg gtgtttctca tccaggataa agaatacgcg gagaaagacc ggccggagct
     4261 gcggctggaa acgaagaaca tcgatgaagt gtatgcgcac gtgaagtcaa aattcccgga
     4321 gctgctgcat ccgaacatga aggaagtgac gctgcgcccg tggggggcaa gggagctcgg
     4381 actgctggat gaatcaggcg tgtgcgtggt tgtgcagcaa tggggataac aagctgaaaa
     4441 ctgttttaac cacgaagaac ataaaggttt tcacgaagcg cacgaagaaa aataaaaaca
     4501 acccgaaacc cgaaactaaa aacggtttta accacgaaga acacaaaggt tttcacgaag
     4561 cgcacgaaga aaaaaacaac tcgaaatccg aaactaaaaa ctgttttaac cacgaaggac
     4621 acgaagtttt cacaaagggg catgaagaaa aacaaaacca acccgaaacc cgaaatccga
     4681 aactaaaaac tgttttaacc aggaagaaca caaaggtttt cacaaagcgc acgaagaaaa
     4741 acaaaaacaa cccgaaatcc gaaactaaaa actgttttaa ccacgaagga cacaaagcgt
     4801 ttcgcaaagc gcacgaagga aaacaggaaa caattcaagt gacaaacaac caaccgaaac
     4861 cggttagccc gaaacccgta tctcccttcc cctgttactt tcactgatat ccactttcac
     4921 tgcattttcc ggcaagacat tttcgatgat ctccggccat tcgatgaagc agtaatttcc
     4981 gctgtagaga taatcctcca gcccgatatc gagcgcctcc tgcactgttt ttacacggta
     5041 cagatcgaaa tgataaacgg gatcatcctg tgcgtcgcgg tattcgttca ccaatgaaaa
     5101 cgtcgggctc gacatcgctt cttttacacc gag
//
LOCUS       DASTSF010000154         2809 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_947360,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000154 DASTSF010000000
VERSION     DASTSF010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2809)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2809)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2809
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_947360"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..201)
                     /locus_tag="VFU15_04520"
     CDS             complement(<1..201)
                     /locus_tag="VFU15_04520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717069.1"
                     /translation="MKRTLLPVFALMALPVAGLHAQMMTDSLNYTGGIQTYTVPCGVT
                     SITIECYGAQGGSGATGGNSSTG"
     gene            392..1639
                     /locus_tag="VFU15_04525"
     CDS             392..1639
                     /locus_tag="VFU15_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016195647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEU4717070.1"
                     /translation="MKPVKRKIFNDPVYGFVTVPTELVFKLIDHPYFQRLRRIRQLGL
                     TSLVYPGANHTRFHHAMGAMHLMQDAIEVIRSKGHTITAEEAEGVSIAILLHDIGHGP
                     FSHALETSIVHNITHEDISALFMDKLNEEFGGKLSLAIRIFRDRYSKKFLHQLVSSQL
                     DLDRLDYLLRDSFFTGVSEGIISSDRIVKMMNVVNDRLVVEDKGIYSIEKFIIARRLM
                     YWQVYLHKTVLSAEHLLVNILHRAKALAENGQELFCSPALRMFLYNRFTKKAFLENPG
                     VLENFAKLDDFDIMTSVKVWTEHSDRILAFLCNNLVNRHLYAVELQNKPFSASYVEDL
                     RKKAEKQFRLKKKEIPYYVFTGAVQNKAYTSDNIRINVLFKNGSVMDIAEAADLFNIS
                     VLAHPVKKYFLCYPKSLRPPKND"
     gene            1632..>2809
                     /locus_tag="VFU15_04530"
     CDS             1632..>2809
                     /locus_tag="VFU15_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012929360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TCR/Tet family MFS transporter"
                     /protein_id="HEU4717071.1"
                     /translation="MTEKPNNKALLFIFLTLLIDVIGLGIIIPVMPKLIMTIAHCDDR
                     MAAIYGAQMTLAFSGMQFGFSPLLGSLSDRYGRRPVLLCSLLAFGLDYLILGFANSIP
                     FLFIGRFIAGATGASFTTATAYIADISTPETRAKNFGMVGAAFGLGFIIGPAVGGLLG
                     SYGTRVPFFAAATLSLINTLYGYFVLPESLKPENRRRFEWKRANPLGALRHLRRYPML
                     IGLALSYMLINLAGQSLPTTWSFFGTYQFGWDEKMIGYSMGAVGIAVASVQGGLNRVL
                     IPKLGERRAVVIGFSIYAVGMAICASSVATWMMFVGIIPLALGGLAGPSLQSMMSGHV
                     PPNEQGELMGTLTSLMSIDLVIAPLIFPWLFYHFSDPAASPRVPGAAFYCSSALTIVA
                     L"
BASE COUNT          592 a          822 c          736 g          659 t
ORIGIN      
        1 acccgtggaa ctgtttccgc ccgttgcgcc ggatcctccc tgcgcaccat agcattcgat
       61 cgtgattgac gttacaccgc agggcacagt gtacgtctgg attccgcccg tatagttcag
      121 gctgtccgtc atcatctgcg catgcagacc tgcaacgggc aatgccatca aggcaaaaac
      181 cgggagtagt gttcttttca taggattgat ttttatcaat attgatgagt cgcgaaagta
      241 gtaaaaatgt agcgccgtga atagtgccag gacctaatct accggaacgc aggatgattt
      301 tgcaccggcg tcaacgcctt cgttgcaatc gtaaaaaagc ggtaacggcc ggatcatgtg
      361 tgaattcgtg tttgtcctac ctttgccccc tgtgaaacca gtcaagcgaa aaattttcaa
      421 cgatcccgtg tacggttttg tcaccgttcc cacggagctg gtttttaagc tcatcgatca
      481 cccgtatttc cagcgcctgc gccgcatacg gcagctcggt ctcacgagcc tcgtttaccc
      541 cggagcaaat cacacgcgtt ttcatcacgc gatgggtgcg atgcatctca tgcaggatgc
      601 gatcgaagtg atccgcagca agggacatac gatcacggcg gaagaagccg aaggtgtgag
      661 catcgccatt cttctccacg acatcggcca cggtccgttc tcgcacgcgc tggagacaag
      721 catcgtgcac aacatcacgc acgaagatat ttccgcgctg ttcatggaca agctcaacga
      781 ggagttcggc gggaaacttt cgcttgccat ccggatcttc cgcgaccgct attccaaaaa
      841 attcctgcac cagctcgttt cctcgcagct cgatctcgac cgcctcgatt acctgctgcg
      901 cgacagcttc ttcaccggcg tttccgaagg catcatcagc agcgaccgca tcgtgaaaat
      961 gatgaacgtc gtgaacgacc ggctcgtggt ggaagacaaa ggcatttatt ccatcgagaa
     1021 attcatcatt gcgcgccggc tgatgtactg gcaggtgtac ctgcacaaga ccgtgctcag
     1081 cgcggagcac ctgctcgtga atattcttca ccgtgcaaaa gcgctggcgg aaaacggcca
     1141 agaacttttc tgctctcccg ccctgcgcat gtttctctac aaccgcttca cgaaaaaagc
     1201 gttcctcgaa aatcccgggg tgctggaaaa tttcgcgaag ctcgatgatt tcgacatcat
     1261 gaccagcgtg aaagtgtgga cggaacacag cgaccgcatt ctcgcttttc tctgcaataa
     1321 cctcgtgaac cgccatctct acgccgtgga gctgcagaac aagccgttca gcgcgagcta
     1381 cgtggaagat ctccggaaaa aagcagagaa gcagttccgg ctgaaaaaga aagagatccc
     1441 gtattacgtt ttcaccggcg cggtgcagaa caaagcctat acttcggaca acatccgcat
     1501 caacgtgctg ttcaagaacg gaagcgtgat ggacattgcc gaagccgccg acctcttcaa
     1561 tatttccgtg ctcgcccacc cggtgaagaa atattttttg tgctacccga aatcgctgag
     1621 accaccaaaa aatgactgaa aaacccaaca acaaagcgct tctgtttatt ttcctcacgc
     1681 tgctcatcga cgtgatcggg ctgggcatta tcattcccgt gatgccgaag ctgatcatga
     1741 ccatcgcgca ttgcgatgat cgcatggcgg cgatctacgg cgcgcagatg acgctggcgt
     1801 tttccggcat gcagttcggt ttttctcctt tgctcggttc gctcagcgac cgttacggca
     1861 ggcgtcccgt gctgctgtgc tcgctgctcg cattcgggct cgattacctc attctcggtt
     1921 tcgcgaacag cattcctttc cttttcatcg gccgtttcat cgccggcgcg acgggtgcga
     1981 gcttcaccac ggccaccgcc tacatagccg acatcagcac accggaaaca cgcgcgaaga
     2041 atttcggcat ggtgggcgcc gcgttcggtc tcgggtttat catcggtcct gccgtgggcg
     2101 gactcctagg cagctacgga acgcgtgttc ctttcttcgc tgccgcgacg ttgtcgctca
     2161 tcaatacgct ctacggatat tttgtgctgc ccgaatcgct caagccggaa aaccggcgca
     2221 ggttcgaatg gaaacgcgcc aacccgctgg gtgcgctgcg tcatcttcgc cgctacccga
     2281 tgctgatcgg cctggcgctt tcgtacatgc tgatcaatct cgcggggcaa tcgctcccga
     2341 cgacgtggtc gtttttcggc acgtaccaat tcggctggga tgaaaaaatg atcggctact
     2401 cgatgggcgc cgtcggtatt gcagtggctt ccgtgcaggg cgggctcaac cgcgtgctca
     2461 tcccgaaact cggtgaacgc agggcggtgg tgatcggttt ttccatttat gccgttggaa
     2521 tggccatatg cgcatcttcc gttgcaacgt ggatgatgtt cgtcgggatc ataccgctgg
     2581 cgctcggcgg acttgccggt ccttccctgc agtcgatgat gtcgggacac gttccgccca
     2641 atgagcaggg agagctcatg ggaacgctca cgagcctgat gagcatcgat cttgtcatcg
     2701 cgccgctcat ctttccctgg ctcttctatc atttttccga tccggcggcc agtccgcgtg
     2761 taccgggtgc cgctttttac tgctcgtctg cgctcaccat cgtggcgct
//
LOCUS       DASTSF010000155         2679 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_598072,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000155 DASTSF010000000
VERSION     DASTSF010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2679
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_598072"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..592)
                     /locus_tag="VFU15_04535"
     CDS             complement(<1..592)
                     /locus_tag="VFU15_04535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717072.1"
                     /translation="MKNLIALSESLNLAEIKLVKHLCKHKSNDDPHKKEKLFDIVLKN
                     KPVSDAAAARELGYARADNSFHNLKQRLRADIFSVLLMQKGTSKFSTPYAQALFECRR
                     NLLHGEVLMSRGVYEEAADLLSKALKTAGRFQLYAEQMQIEDLLRNHTALSGNIYAFR
                     TANDSIEKNYRLLGQMMEAKKKHYELTDPRLMGASSL"
     gene            complement(958..1284)
                     /locus_tag="VFU15_04540"
     CDS             complement(958..1284)
                     /locus_tag="VFU15_04540"
                     /inference="COORDINATES: protein motif:HMM:NF016296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEU4717073.1"
                     /translation="MKGFIEIADNRKVIVIPISDISRLESQDGYTIIHTKSRQQHVST
                     KHLKEIEKKLDPKIFHRVHKSHVINMQEVRTYEKGNGGYVTMSDGSIVAVSRRKKADF
                     LRELIS"
     gene            complement(1673..>2679)
                     /locus_tag="VFU15_04545"
     CDS             complement(1673..>2679)
                     /locus_tag="VFU15_04545"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HEU4717074.1"
                     /translation="ELDFATGVAKWSDESCRIYGLSPEDNMHSFKSWLSFIHPDDRQR
                     VIGEIQEAEKTGSGTSIKHRIVRPDGSIRYILSESRPEFGPDGRPVGVYGTAHDVTEN
                     TVLEQSLQASYKELETFIYRVSHDLRGPLASIQGLVRVSVLDVKDETALKHIGMIGDL
                     AMKLDGLLTKLVHIMSVRDKKTTNDPVDVVQMVEGILSSLKFIDHFGNIKFSVNSNVK
                     EMRTDSEALRSVLLNLVENAIKYHDYSRKMPFIDINIHRNGSPNVSIEISDNGQGIDE
                     EVKDKVFDMFYRGTTSSKGTGLGLFIVKSTVEKLGGTITLESAKGKGSRFRLSIPVSD
                     "
BASE COUNT          600 a          686 c          686 g          707 t
ORIGIN      
        1 tcagcgagga tgcgcccatc aggcgcggat cggtcagctc gtaatgtttc ttcttcgctt
       61 ccatcatctg cccgagcagc ctgtaatttt tttcgataga gtcgttcgcc gtacggaacg
      121 cataaatatt cccgctgagc gccgtatggt tgcggagaag atcttcgatc tgcatttgtt
      181 ccgcatacag ctggaaccgg cccgcggtct tgagcgcctt cgacagcagg tctgcagctt
      241 cctcgtatac gccgcggctc atcagcactt cgccatggag aagattgcgc ctgcattcga
      301 acagggcctg cgcgtaagga gtggaaaact ttgatgtgcc tttctgcatc agcagaaccg
      361 aaaaaatgtc agcgcgtaaa cgctgcttca ggttgtgaaa cgagttgtcg gccctggcgt
      421 agcccagctc tcgtgcggcg gccgcatcac tgacaggttt gtttttcagg acaatgtcga
      481 acagcttttc ttttttgtgc ggatcgtcat tgcttttgtg cttgcagagg tgctttacca
      541 gctttatttc cgcaaggttc agactttccg aaagtgcaat caggttcttc atacacgcgt
      601 tttcttttca gatgattgat gacaacatat ttcggcaagg aggaagcggt caatccactt
      661 ccgagtaatc ccagggtccg caacgcgtgg aaaacggcga caggtaagac cagctggggg
      721 cgacgaaacc gccacccccg cttttgcgcg ggtaatttct ttgaataaaa gtcattgtgt
      781 aggggtatta agtgaaaatc agtcgggggg agaaaatgcg gtatggaaaa acaagcttga
      841 catatacggg tactaattgt atgcaactaa atgaaaagaa attgtaaatc aaatccggaa
      901 tcgaagaacg gtatcaaaca gtgaatttcc ggtaatgaaa accaattaac aggcaaatca
      961 ggagatgagc tccctgagga agtccgcctt cttgcgccgt gagaccgcaa cgatgcttcc
     1021 gtcgctcatg gtaacgtacc cgccgttccc tttttcgtac gtgcgcactt cctgcatgtt
     1081 gatgacatgc gatttgtgca cgcggtgaaa tattttcgga tcgagctttt tctcgatctc
     1141 tttcaggtgc ttggtgctga cgtgctgctg ccggcttttc gtgtggatga tggtgtaccc
     1201 gtcctgcgat tcgagccgcg atatatcgct gatcgggatg acgatcacct tccggttgtc
     1261 tgcgatttca atgaatcctt tcatgggtca aagggtgttt tagggcgata aagacagaca
     1321 gtcctgcaga tcatccgctg taaagcgcat gccgctgaaa agtccgcagg tacagacgat
     1381 aaatgaacaa gcgacctctc gctgaccgtc cgggagtaaa cgatcaacca cattccggca
     1441 agcttcggga ggggggattc cttctgcaga gcggggttat ttcccaggca cgttgcccgg
     1501 cactttgcag ccggagggtc ttcacgattg cagagttacg ctaatatagt ttaatcaatt
     1561 atctctccaa gtaatttctt cgttaaaatt tcaatactta cactatgtaa gcaattatta
     1621 cgacagtttt taaaacaatt atttaaaagg ctgcgcccat tcctgaaaag attcagtcgc
     1681 tgaccgggat gctcaggcgg aaccggcttc cttttccttt cgcgctctcg agcgtgatcg
     1741 tcccgccgag cttttcgacg gtggatttga caatgaaaag cccaaggccc gttccttttg
     1801 aagatgttgt tccccggtag aacatgtcga acactttatc cttcacttcc tcgtcgatgc
     1861 cctggccgtt gtcgctgatc tcgatgctga cattgggcga tccgttcctg tgaatgttga
     1921 tgtcgatgaa tggcatcttc cgggaataat cgtggtactt gatggcgttc tcgaccaggt
     1981 tcagcagcac gctccgcaat gcctcgctgt ctgtccgcat ctcttttacg ttgctgttca
     2041 ccgaaaattt tatgttgccg aaatgatcga tgaacttcag cgagcttaat attccttcga
     2101 ccatctgcac aacgtcgacg ggatcgttcg tcgttttttt gtcgcgcaca gacatgatat
     2161 gcacgagctt cgtgagcagc ccgtcgagct tcatcgcgag gtcgccgatc ataccgatgt
     2221 gcttcagcgc cgtttcgtct tttacatcca gcaccgaaac ccgcacgagc ccctggatgg
     2281 aagccagcgg tccgcggagg tcatgcgaga cccgataaat aaatgtttcg agctccttgt
     2341 aggatgcctg caggctctgc tcgagaacgg tattctcggt tacgtcatgc gccgtaccgt
     2401 aaacgcccac aggccggccg tcgggtccga attccggcct gctttcggaa aggatataac
     2461 ggatcgagcc gtccgggcgg acaatgcggt gtttgatgga agttccgctg ccggtttttt
     2521 ccgcttcctg tatttcaccg atcacccgct ggcggtcgtc cgggtgaatg aacgaaagcc
     2581 atgatttgaa cgagtgcata ttatcttccg gtgaaagtcc gtagatcctg cacgattcat
     2641 ccgaccactt cgcgacaccc gtcgcaaaat cgagttccc
//
LOCUS       DASTSF010000156         5607 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1394760,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000156 DASTSF010000000
VERSION     DASTSF010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5607
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1394760"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..398
                     /locus_tag="VFU15_04550"
     CDS             <1..398
                     /locus_tag="VFU15_04550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717075.1"
                     /translation="DTGLSTVFSGKKKTAEKIDATLEDIDIHAIDYEQEISRAVSVKD
                     FRLAVRLWFLRTLKKMTDQELVRYSIDKTNSDYYYELSGTPYQENFSNVSRVYDFVWY
                     GEFEIDENKYRDAETKFEQVNRQLSAKPQ"
     gene            403..1623
                     /locus_tag="VFU15_04555"
     CDS             403..1623
                     /locus_tag="VFU15_04555"
                     /inference="COORDINATES: protein motif:HMM:NF025617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4350 domain-containing protein"
                     /protein_id="HEU4717076.1"
                     /translation="MKKKYWPYYIALALVLTALYFISAEANKKEPEWREDYTYASKEP
                     YGCYVTRDYVENMLHSDVRTLDETAFDHLFDNSYSNTNYVFVNGVFEPSKEDVEELCK
                     YVSAGNNVFISARNFGELGDSLDVQTLDNMMIEMNNNPSTVHGLIDSSYVSTEANLFN
                     PALHLQKNAQFRFTHTSSVLVRFDTAHVVILGGDERGRANYIRVPFGQGSFFLHTLPD
                     AFVNYYAAQHNNADYLFGVLSYLPDQPTILDEHYKFGRKENTDPRRYLLQEPALRLAY
                     ITLIIAGLVALFFGGKRKQRAVPVVAPVTNTTLEFVEQVGALYYSHGNHSDIMLKKIN
                     YFLESVRARFYVSTNVFDEKFLERIENLSGIPAGPVRTLFAAIDHYRISVCSENDLKK
                     LESLIWDFNQRSKR"
     gene            1686..2630
                     /locus_tag="VFU15_04560"
     CDS             1686..2630
                     /locus_tag="VFU15_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019989227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEU4717077.1"
                     /translation="MDDLREGVASIRAEIGKIIVGQERTVDLLIAALLSDGHVLIEGV
                     PGVAKTLTAKLLARTVSVKFSRIQFTPDLMPSDVIGTSVFNLKTNEFEFKHGPVFANI
                     VLIDEINRAPAKTQSALFESMEERQVTVDGKTWKLGSPFMVVATQNPVEQEGTYRLPE
                     AQLDRFLFKINVDYPTLQDETKILADFHSRKNHIDIDSVKAVLTAEQLKSFREKVYSL
                     HVEPNLLGYIAQIVNQTRNNSALYLGASPRASLAILTSAKAIAAMRGRDFVTPDDIRE
                     VAMPVMQHRVMLTPEKEMEGQTSRDVIRQIIQQVEIPR"
     gene            2637..3971
                     /locus_tag="VFU15_04565"
     CDS             2637..3971
                     /locus_tag="VFU15_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009284751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEU4717078.1"
                     /translation="MIKFLRSLFFTPRFYIMCGAMAVFATASWFWPVYYPFAELAAMV
                     LPALLVIDFIMLWSRKNGVFARRDMMEKLSNGDLNDIRITIENRYPFPVFIDVIDELP
                     VQFQARDLKFRVYLPPFKSKLLTYRVRPFRRGEYVFGSVNVLTSGVPGLLKRRFRYDN
                     AQAVPVYPSFMQMRRYELLAISNRLTEAGVKKIRRIGQNREFEQIREYVPGDDPRVVN
                     WKATARKARLMVNTYQDEKSQQVYALIDMGRVMRMPFEEMTLLDYAINASLVISNIAM
                     LKYDKAGVITFSNKVHSILPAERKGLQMYKIMELLYASKTGFLESDYGRLYANIKQRI
                     TQRSLLLLFTNFETLSSMQRQMQYFRRMAKDHLLVVIFFENTELKAFLDKKSESVQEI
                     YYKTVAEKFAYEKKLIVKELEAYGIHAVYTSPENLTVNTINKYLELKARGLI"
     gene            complement(4018..4632)
                     /locus_tag="VFU15_04570"
     CDS             complement(4018..4632)
                     /locus_tag="VFU15_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014682567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT-like domain-containing protein"
                     /protein_id="HEU4717079.1"
                     /translation="MDQLTRNQSILGKYIPAPAVPLIAEWIYHFDFKLKIKKSRQSRY
                     GDYRPPVRGTNHLITVNNDLNQYAFLLTLVHEIAHLLCFQRHGNNVKAHGHEWKQAYK
                     ELMRPFMRLEIFPHDVRAAIIGYMQNPGASTCSDDNLMRVLKRYDANNDLVHLETLPM
                     YAEFIYNERSFIKGPRVRTRFQCKEKLSSAVYFFAALAEVKAVS"
     gene            complement(4671..>5607)
                     /locus_tag="VFU15_04575"
     CDS             complement(4671..>5607)
                     /locus_tag="VFU15_04575"
                     /inference="COORDINATES: protein motif:HMM:NF014665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0016653 - oxidoreductase activity, acting
                     on NAD(P)H, heme protein as acceptor [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HEU4717080.1"
                     /translation="WPKCFGYYIPPTDPSQVEFHSGHEYSKGMMIIVNDTLWRAKDDF
                     HSGANFRDGDHWEKYPVHDYAKFFVAHTWTEYINRLFGALSGFSMFLLMFIALLRIRK
                     DWKTFAVMVAGMCMMAFVIWLGAVVVNTNLAPFKITFHMLSAMVMVAIVILVQARVRA
                     MCGEQKHVVPKFARILFFLALLLTLDQVIFGTQVRQQIDTIYSNMSGLSRETWIAQLN
                     GVYLYHKIMAGVVMIVNIALFWFMYRTKPEGKTKWLSYGLLLVMLGEYGAGVFMHNFA
                     IPAYAQPVHLLLAMILFGVQLGLIVRTRSGEIVQN"
BASE COUNT         1411 a         1523 c         1468 g         1205 t
ORIGIN      
        1 ccgataccgg tctcagcacg gtgttctcgg gcaaaaagaa aactgcggaa aagatcgacg
       61 cgacacttga agacatagac attcacgcga tcgattacga acaggaaatc tcgcgcgccg
      121 tatcggtgaa agatttccgc cttgccgtcc gcctgtggtt tctccgcacc ctgaaaaaaa
      181 tgaccgacca ggaacttgtc cgttacagca tcgacaaaac caacagcgat tattactacg
      241 agctttcggg aacgccttac caggaaaatt tcagcaacgt ctcgcgtgtg tatgattttg
      301 tgtggtacgg cgagttcgag atcgacgaga ataaataccg cgatgccgag acaaagttcg
      361 agcaggtgaa ccgccagctt tcagcaaaac cacaatgagc aggtgaaaaa gaaatactgg
      421 ccatattaca ttgctcttgc gctcgtgctg accgcgctct atttcatttc cgccgaagcg
      481 aataaaaaag agccggaatg gagagaagat tacacatacg ccagcaaaga accttacggc
      541 tgctacgtaa ccagagatta cgtggagaac atgctccatt ccgatgtgcg cacgctggat
      601 gaaaccgcct tcgatcatct tttcgacaac agctactcga atacaaatta cgttttcgtg
      661 aacggcgttt tcgaaccttc aaaagaagat gtggaagagc tctgcaaata tgtttcggca
      721 ggcaacaacg tttttatttc cgcacgcaac ttcggagaac tgggagattc gctcgatgtg
      781 cagaccctcg acaacatgat gatcgagatg aacaacaacc cctcgaccgt acacgggctc
      841 atcgacagca gctatgtttc aacggaagcg aacctgttca atcctgcgct gcacctgcaa
      901 aagaatgcgc aattccggtt tacgcacacc tcatcggtcc tcgtgcgttt cgacacggcg
      961 catgtggtca tcctcggtgg cgatgaaaga ggccgtgcga attatatccg cgtcccgttc
     1021 ggacagggca gcttttttct gcacacgctg ccggatgcat tcgtcaacta ctacgcggcg
     1081 cagcacaaca acgcggatta tcttttcggc gtgctcagct acctgcccga ccagccgacc
     1141 attctcgatg agcattacaa attcgggcgg aaggaaaata cagacccgag aagatatctc
     1201 ctgcaggaac ctgcattgcg tctcgcctat atcacgctca tcattgccgg gctcgtggcg
     1261 cttttcttcg ggggaaagcg aaagcagcgt gcggttcccg tcgtcgcacc cgtgacgaac
     1321 acgacactgg aattcgtgga gcaggtgggc gcgctttatt acagtcacgg caaccacagc
     1381 gacatcatgc tgaagaagat caattatttt ctcgaatccg tgcgtgcaag attttacgtc
     1441 agcacaaacg tgttcgatga aaaatttctc gaacgcatcg aaaatctttc gggcatcccc
     1501 gcaggaccgg tgcggacgct gttcgcagcc atcgaccatt accgtatttc cgtctgttcg
     1561 gaaaacgacc tgaaaaaact cgaatcgctc atctgggatt tcaaccagcg gagcaaacgc
     1621 taatactacc accaaataaa aaaacgatga cagacaactc gcaatattac cagtcgcgga
     1681 tcccgatgga cgacctgcgc gaaggcgtgg ccagtatccg cgcggagatc gggaagatca
     1741 tcgtcgggca ggaacgcacc gtcgatcttc tcatcgcggc gctgctttcc gacgggcacg
     1801 tcctgatcga aggcgtgccg ggcgtggcga aaacgctcac ggcaaagctt ctcgcacgaa
     1861 ccgtcagcgt gaaattctcg cgtatccagt tcactcccga cctcatgcct tcggacgtga
     1921 tcggtacgag cgtgttcaat ctcaaaacga acgagttcga attcaaacac ggaccggtct
     1981 tcgccaacat cgtgctcatc gacgagatca accgggcgcc ggcgaaaacg caatccgcgc
     2041 ttttcgaatc gatggaggaa cgccaggtga ctgtcgacgg aaaaacgtgg aaactcggtt
     2101 cgccgttcat ggtcgtggcg acgcagaatc ccgtcgagca ggaaggaacg taccgcctgc
     2161 cggaagcgca gctcgaccgt ttcctgttca agatcaacgt cgattacccg acgctgcagg
     2221 acgagacgaa gatcctcgcc gatttccatt cgcggaaaaa tcacatcgac atcgatagcg
     2281 tgaaagcagt gctgaccgca gagcagctga agagcttccg cgagaaagtg tacagtcttc
     2341 acgttgagcc gaacctgctc gggtacattg cgcagatcgt caaccagacg cgcaacaaca
     2401 gcgcgctgta cctcggcgcg tcgccgcgcg cttcgctggc cattctcact tcggcgaagg
     2461 cgatagccgc gatgcgcggc agggatttcg tgacgccgga cgatatccgg gaagtcgcca
     2521 tgcccgtgat gcagcaccgc gtgatgctca ctccggaaaa agaaatggaa ggacagacat
     2581 cgcgcgacgt catccgccag atcatccagc aggtggaaat accacggtaa acgcacatga
     2641 taaaatttct ccggtcgctt tttttcacac cacgcttcta cattatgtgc ggagcgatgg
     2701 ccgtgtttgc cacggcgtcg tggttctggc cggtgtatta tccttttgcg gagctggcgg
     2761 cgatggtgct gcccgccctg ctcgtgattg atttcattat gctctggtcg cggaaaaacg
     2821 gcgtgttcgc ccggcgcgac atgatggaaa agctttccaa cggcgatctc aacgacatcc
     2881 gcatcacgat cgaaaatcgt tatcctttcc cggttttcat cgacgttatc gatgagctcc
     2941 cggtgcagtt ccaggcgcgc gacctgaaat tccgcgttta cctccctcct ttcaaatcga
     3001 aactgctgac gtaccgcgtg cgcccgttca ggcgcggcga atatgtgttc ggatcggtga
     3061 acgtgctcac ttccggcgtg cccggcctgc tgaagcgccg tttccgttat gacaatgcgc
     3121 aggcggtgcc ggtatatcct tcgttcatgc agatgcgccg ctatgagctg ctcgccattt
     3181 ccaaccggct gacggaagca ggcgtcaaaa agatcaggcg gatcgggcag aaccgtgaat
     3241 tcgagcagat acgcgaatac gttcccggcg atgatccgcg cgtggtgaac tggaaagcga
     3301 cggcgagaaa agcccggctg atggtgaata cttaccagga tgaaaaatcg cagcaggtgt
     3361 atgcgctgat cgacatggga cgcgtcatgc gcatgccgtt cgaggaaatg acgcttctcg
     3421 attacgccat caacgccagc ctcgtcattt cgaatatcgc gatgctgaag tacgacaagg
     3481 ccggcgtgat caccttttcg aacaaggtgc acagcatcct gcccgcggaa cggaaagggc
     3541 tgcagatgta caagatcatg gagctgctgt acgcgtcgaa aacaggtttt ctcgaatcgg
     3601 actacggaag gttatacgcg aacatcaagc agcgcatcac gcagcggagc ctgctgctgc
     3661 tgttcacgaa tttcgaaacg ctctcgagca tgcagcggca gatgcagtat ttccgcagga
     3721 tggcgaaaga tcacctgctc gtcgtgatct ttttcgagaa tacggagctg aaagcgttcc
     3781 tcgacaaaaa atcggaatcc gtacaggaaa tttattacaa aacggtcgct gaaaaattcg
     3841 cgtacgagaa aaaactcatc gtgaaagagc tggaagcata cggcattcat gcagtttaca
     3901 cttcaccgga aaatcttacg gtaaatacga tcaacaagta tcttgaatta aaagcgagag
     3961 ggcttatctg atcgcctggt ctcattcgct atctactccc ggaaatatcc tcttctttta
     4021 agaaaccgct ttcacctccg ccagcgctgc gaaaaaataa acggcgctgc tcagcttctc
     4081 tttacactgg aaccgtgtgc gcacgcgcgg acctttgatg aagcttcgtt cattgtaaat
     4141 gaattcggcg tacatcggca gcgtctcgag gtgaacgaga tcgttgttcg catcgtaacg
     4201 cttcagcacg cgcatgaggt tatcgtccga gcatgtcgat gcgccgggat tctgcatgta
     4261 gccgatgatg gcggcgcgaa cgtcgtgcgg aaaaatttca agacgcatga acggtcgcat
     4321 cagctccttg tatgcctgct tccattcatg cccgtgcgct tttacattgt tgccgtgccg
     4381 ctggaaacaa agcaggtgtg cgatctcgtg cacgagcgta agcaggaacg catactggtt
     4441 aagatcgttg ttgacagtaa taagatgatt tgttccgcgg acaggcgggc gataatcgcc
     4501 gtacctcgac tggcggcttt ttttgatctt cagcttgaaa tcgaaatggt agatccattc
     4561 ggcgataagc ggaacggcag gagcaggaat gtatttcccg agaattgact gattgcgggt
     4621 cagctgatcc attcgcacaa aactaaaacg gggaacccaa aagttgtttt tcagttttga
     4681 acaatttcac ctgaccgcgt gcggacgatc agtccgagct gaacaccgaa aaggatcatg
     4741 gccaggagaa gatgaaccgg ctgcgcgtag gccggaatgg cgaagttgtg catgaacaca
     4801 cctgcaccgt attcgccgag cataacgaga agcaggccat acgaaagcca ttttgttttc
     4861 ccttccggct ttgttctgta catgaaccag aacagcgcga tattcacgat catcaccacg
     4921 cccgccatga tcttgtgata caggtaaacg ccgttgagct gcgcgatcca cgtttcacgc
     4981 gaaagtccgc tcatgttgct gtagatggta tcgatctgct ggcggacctg cgtgccgaag
     5041 atcacctggt cgagcgtgag cagcagcgca aggaaaaaaa gaatccgggc gaatttcgga
     5101 acaacatgct tctgttcccc gcacatcgca cgcacgcgcg cctgcacgag gatgacgatg
     5161 gccaccatca ccatcgcgct cagcatgtgg aaggtgatct tgaacggagc gagattcgta
     5221 ttcaccacaa cggcgcccag ccagatcacg aacgccatca tgcacatgcc ggcaaccatg
     5281 accgcgaatg ttttccagtc tttacggatg cgcagcagcg cgatgaacat gagcaggaac
     5341 atggagaacc cgctcagcgc accgaaaaga cggttgatgt attccgtcca cgtgtgcgcc
     5401 acgaaaaatt tcgcgtaatc gtgcacggga tatttttccc agtgatcacc gtcgcggaaa
     5461 ttcgctcccg aatgaaaatc gtccttcgcc cgccaaagcg tatcgttgac gatgatcatc
     5521 atccctttcg aatactcgtg accgctgtga aattccacct gcgaaggatc cgtgggcgga
     5581 atgtaatagc cgaagcattt cggccag
//
LOCUS       DASTSF010000157         2319 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1501975,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000157 DASTSF010000000
VERSION     DASTSF010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2319
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1501975"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..929)
                     /locus_tag="VFU15_04580"
     CDS             complement(<1..929)
                     /locus_tag="VFU15_04580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEU4717081.1"
                     /translation="MKRKTLFLFAVFISAACFSQTRELSYFIDNGIANSPLLKDYAGQ
                     VQKNSLDSSLLRSQLRPQADAVASVMAAPSYNGWGYDEAITNGYNIGMQAVVRQDLFA
                     GKIYAPQYEALRLQSSSAKNSSAITGHELKRDITNQYLAACATLNDITYQQSVIQLLS
                     GEESALKSFVASGIYRQTDFLAFEIEKQQEEITLSQLQLQYRSDISELNALCGISDTS
                     YYEVALPPLAPSFAFSPAASPFLGQFRIDSLRILNSRNLVDAEYRPHFGWYADAGLLA
                     SNPATMYRNFGFSFGFFFSVPLYDGHRRNFNYS"
     gene            complement(1025..1798)
                     /locus_tag="VFU15_04585"
     CDS             complement(1025..1798)
                     /locus_tag="VFU15_04585"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008142115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEU4717082.1"
                     /translation="MRIFGLIGFPLSHSFSEKYFTEKFRRENIPDAVYRLFPLENAND
                     FPSFLQANPGIAGLNITIPYKEKIIPFLDELDETAKAVGAVNVIQIREDGGKKILEGY
                     NSDVYGFRQSLKPFLTSAHERALILGTGGASKAVEYVLKQIGVQCIFASREKKNIPGK
                     NILTYAEVNEYVLSACRLIVNATPCGMFPDVKSAPPLPYEFITPEHFLYDLIYNPAET
                     EFMKRGKEKGATVMNGMDMLKLQAERAWTIWNPGLNPGF"
     gene            complement(1800..>2319)
                     /locus_tag="VFU15_04590"
     CDS             complement(1800..>2319)
                     /locus_tag="VFU15_04590"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEU4717083.1"
                     /translation="SLLFAAGALCARSADHANPAMNIGALIGLLSVAAVLGDNSNYAI
                     GRFLGKFAVNMRMGKRKLVRQEHIDRTHSFFEKYGTKAIIMARFVPIVRTFTPFVAGV
                     GKMDYSRKFLPFDIAGGILWISTMSLAGFFLGTNDFVKRHFEMVVVAIIVISVLPMVF
                     GYFRARAQRKKA"
BASE COUNT          536 a          582 c          665 g          536 t
ORIGIN      
        1 ttgctgtaat tgaagtttct ccggtgaccg tcatataacg ggacggaaaa gaaaaatccg
       61 aagctgaacc cgaaattgcg gtacatcgtg gcgggattcg aagcaagcag cccggcgtcg
      121 gcataccacc cgaaatgcgg acggtactca gcgtcgacca ggttgcggga attgagaatg
      181 cgaaggctgt cgatgcgaaa ctgcccgaga aagggcgatg cggccggaga aaaagcgaag
      241 gaaggtgcca gtggcggaag cgcaacttcg taatacgagg tgtcggaaat cccgcagagc
      301 gcattgagct cgctgatgtc ggatcggtat tgaagctgca gctgcgaaag ggtgatctct
      361 tcctgctgct tctcgatctc gaacgcgagg aaatccgtct gcctgtaaat gccgctggcg
      421 acgaaacttt tcagcgccga ctcttcgccg gaaagaagct ggatcacgct ctgctggtag
      481 gtaatgtcgt tgagcgtggc gcaggcggcg aggtattgat tcgtgatgtc tcgcttcagt
      541 tcgtgtccgg tgatcgcgga ggaattcttc gcggagctgc tttgcaggcg cagcgcttcg
      601 tactgcggcg cataaatttt cccggcgaaa agatcctgcc tcaccacggc ctgcatgccg
      661 atgttgtagc cgttggtgat cgcttcgtcg taaccccagc cgttgtaaga aggcgccgcc
      721 ataacggaag cgacggcgtc cgcctgcggg cgaagctgcg aacgaagcaa tgagctgtcg
      781 agtgaatttt tctgcacctg tcccgcataa tctttcagca gcggggaatt ggcgatgccg
      841 ttgtcgatga agtagctgag ctcccgcgtt tgcgaaaaac acgctgcgga aataaatacg
      901 gcgaaaagaa aaagggtttt cctcttcata agctcttcga aggtaaaagc aaattctgtg
      961 tgaattctgg tgctttcccg gcacttattc agttcaaatg ttaaacgaat gccggattaa
     1021 gaattcaaaa tccgggattg aggccggggt tccagatcgt ccacgcccgt tctgcctgca
     1081 gcttcagcat atccatcccg ttcatcaccg tggcgccttt ttcttttccg cgtttcatga
     1141 attccgtttc ggcgggattg taaatgaggt cgtacaggaa atgttccggg gtgatgaatt
     1201 catagggaag gggaggcgcg ctttttacgt ccgggaacat gccgcagggt gttgcgttca
     1261 cgataaggcg gcaggcgctc agtacgtatt cgttcacctc cgcatacgta agaatatttt
     1321 tcccgggtat gtttttcttt tcccgcgaag caaaaatgca ttgaacaccg atctgcttca
     1381 gcacgtattc cacggccttt gacgcgcccc cggtgccgag gatgagcgcg cgttcgtgtg
     1441 cggaagtgag aaaaggtttc agcgactggc ggaagccgta cacgtcggaa ttataacctt
     1501 ccaggatttt tttcccgccg tcttccctga tttgaatgac gttcacggca cccacggctt
     1561 ttgcagtttc atcgagttcg tcgaggaacg gaatgatctt ttctttgtac ggaatggtga
     1621 tgttgagtcc cgctatgccg gggttcgcct gcaggaagga aggaaaatcg ttcgcgtttt
     1681 ccaatggaaa aagacggtaa acggcatccg ggatattttc ccgccggaat ttttccgtga
     1741 aatatttttc ggagaacgaa tgggaaagcg ggaagccgat gagaccgaaa atgcgcaagt
     1801 caggcttttt ttcgttgggc gcgcgcccgg aaatacccga acaccatggg aaggacagaa
     1861 atgacgatga tcgcgacgac caccatctcg aaatgccttt tcacgaaatc attggtgccg
     1921 aggaaaaatc ccgcgagcga catggtgctg atccagagaa tcccgcccgc gatatcgaac
     1981 gggaggaact tacggctgta atccattttt cccacgcccg ccacgaacgg ggtgaatgtg
     2041 cgcacgatgg gaacaaaacg cgccatgatg atggctttcg tgccgtattt ttcgaagaag
     2101 ctgtgtgtgc ggtcgatgtg ttcctgcctg accagtttgc gttttcccat ccgcatgttc
     2161 acggcgaatt ttccgagaaa gcgaccgatc gcataattcg aattgtcgcc gagaaccgcc
     2221 gccaccgaaa gcagcccgat cagcgcaccg atgttcatcg ccgggttcgc gtgatctgcg
     2281 ctgcgtgcgc acaaagcgcc tgccgcgaaa agaagcgaa
//
LOCUS       DASTSF010000158         3470 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1347073,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000158 DASTSF010000000
VERSION     DASTSF010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3470
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1347073"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..478
                     /locus_tag="VFU15_04595"
     CDS             <1..478
                     /locus_tag="VFU15_04595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717084.1"
                     /translation="YIAVIIGNQRCLSVKSNKSQKNHYGLYINMVPLEGGLFNQFLLL
                     DSSNRAYYLFDSIDYCAIGKSKLIENFRQQGDFHVYGDSIKFSFDSCASITSHATLYI
                     HGQNIEINQKQFDHDTRQEYRGKFDGDQISFVITNVNAKHGNSFRKENYIRCGAAR"
     gene            744..1376
                     /locus_tag="VFU15_04600"
     CDS             744..1376
                     /locus_tag="VFU15_04600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717085.1"
                     /translation="MQPIVSIRKPCSEDWTKMTPQEQGRHCDKCCKVVVDFSGSSDQQ
                     IIDYLEHHSGSRVCGRFRKEQVSVPAVSATIRKSRVKVFLAALVFVFGAFLFTSCNNN
                     GEVVGKFSSAPDSAMRANEQAMQHTLGDTILPREKADSPVKKIPGKCIQDPGYKVMGD
                     VAILPVDTSAVKPGGGTVPQDNHPKGEVMLRDTTSPADNGVQGQIMLNPR"
     gene            1404..1919
                     /locus_tag="VFU15_04605"
     CDS             1404..1919
                     /locus_tag="VFU15_04605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717086.1"
                     /translation="MLRIDNPCSESWEKMTPAEQGRFCDKCSLGVIDFTQKSQEEIIA
                     FIRNRNGQRTCGRFRRSQLAPVKFRWRMQKFAAAALLVFGAFLFSSCGNKNDGDGHVM
                     GDVPYQPDDSTRMAIEKKAIADSRRSADSVAKVQNDADRNNTVYHLSSADSAKAADSA
                     ARSRNGVRDFK"
     gene            1965..2564
                     /locus_tag="VFU15_04610"
     CDS             1965..2564
                     /locus_tag="VFU15_04610"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEU4717087.1"
                     /translation="MILPVRKLFPVAAVFAFLLFSCKPDDPAPQFTDSTDGSSPGVDT
                     FVVSRSHALSPADQQEIDKDTQETRQIVNDAVTLINGGQAPSSSNVNDDDIVIAPDDP
                     PTFPGGQDAMDEYIHRKLVYPLTAMQNDIKGTVTLKVVIEKDGQVGAISIVRGLGYGC
                     DEAAVECVRGMPKWIPAKKAGYNVRTTVTIPLYFAKDDD"
     gene            2661..3323
                     /locus_tag="VFU15_04615"
     CDS             2661..3323
                     /locus_tag="VFU15_04615"
                     /inference="COORDINATES: protein motif:HMM:NF015871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HEU4717088.1"
                     /translation="MKEKLSLALNAILIVAVIILFYLHFSSRAAIADVKKVQDSSATS
                     LTFRIPKNLAGARVLYVNIDSISTKYKAFADLSSSEGTKIDTKMQQYQRRAMALQQRM
                     NDLNTKAQNNLISGDDAQKEQDAIDKEQKALNAMEIEINDMQTKAQEKNQVITEEVFK
                     YFNQYSREKKIDFILAYGAGSPIIYANDSLDVTNDVVDALNANYDANQKNNPMPLAPK
                     GN"
BASE COUNT          929 a          855 c          903 g          783 t
ORIGIN      
        1 gtatatagcg gttattatcg gaaaccagag atgcttaagc gtaaagtcga ataaatctca
       61 aaaaaatcat tacggactct atattaatat ggtcccatta gaaggagggc tattcaatca
      121 gtttcttctc ctggacagtt caaacagagc ttactattta tttgattcaa tcgattattg
      181 tgctattggg aaaagtaaat taatcgagaa ttttcggcag cagggagatt ttcatgttta
      241 tggcgattct ataaaatttt cctttgatag ttgcgcctca attacctctc atgctacgct
      301 gtatattcat ggacaaaata ttgagatcaa tcaaaagcag ttcgaccatg ataccagaca
      361 ggagtatcgt ggcaaatttg atggagatca gatctccttt gtcataacta acgttaatgc
      421 taaacatgga aattctttta ggaaagaaaa ttatatcaga tgtggtgccg ccagataaac
      481 agacattaaa accgtttcca aagttcaaaa cttgagggga gacaatatga atgtcgttct
      541 gcattgtatt ttaactctcc caataacgtt acctggaagt aaaaagagca gaaggaaatt
      601 cctctagccc cgatggaacc gagctcctgc gaggcgacag cgggaggaga ggaaccggga
      661 agcaccgcag ttgcgcttcc ctgaaaaaac gacgccgttt atggaaacaa cctttccact
      721 gcatctattg gcaaaatgcg cttatgcaac ctatcgtttc catccgcaaa ccctgttccg
      781 aagactggac aaaaatgacc ccgcaggagc agggccgtca ttgcgacaag tgctgtaaag
      841 tcgtcgtgga tttttccggc agcagcgacc agcagatcat cgattacctt gagcatcatt
      901 ccgggagccg cgtgtgcggc cgtttccgca aggagcaggt ttccgttccg gcggtttccg
      961 caacgatccg aaagtcgcgc gtgaaagttt ttcttgcagc gctcgttttt gttttcgggg
     1021 cgttcctttt cacgtcatgt aataacaacg gcgaggttgt cgggaaattc tcgtctgcac
     1081 ccgattcggc gatgcgggcc aatgaacagg ccatgcagca tacccttggt gatacgatcc
     1141 ttccgaggga aaaagccgat tcgcccgtga agaaaatccc ggggaaatgc atccaggacc
     1201 ccggctataa ggtgatgggc gacgtggcga tcctgccggt cgatacttca gcggtcaagc
     1261 caggcggtgg gacagttccg caggacaatc acccgaaggg cgaggtcatg ctgcgggata
     1321 cgacgtcgcc tgcggataat ggcgtccagg ggcagatcat gcttaatccc cgctgagttg
     1381 ggcggaattg ctatcttcat tccatgctac gcatcgataa tccatgcagc gaatcgtggg
     1441 agaaaatgac tcccgcggag cagggccgtt tctgcgataa gtgcagtctc ggcgtgatcg
     1501 atttcacaca gaaatcgcag gaagagatca tcgcatttat ccgcaacagg aacgggcagc
     1561 gtacgtgcgg ccgtttcagg agatcacagc tcgcacctgt gaaattccgc tggaggatgc
     1621 agaagtttgc cgccgctgca ctgttggttt tcggggcttt tcttttttct tcgtgcggaa
     1681 ataaaaatga cggtgacggg catgtgatgg gcgatgtgcc ttaccagccg gatgattcca
     1741 cccggatggc gattgaaaaa aaggcgatcg cggactcaag gagatcagcc gacagcgtgg
     1801 cgaaagtcca gaacgatgcg gacaggaaca atacggtcta ccatctttct tccgccgaca
     1861 gcgcaaaagc agccgacagc gcggcacgga gccgtaacgg cgtgcgtgat ttcaaataag
     1921 atgtgacttt ttcgcctcaa acgttttatg cattaaacgc gtttatgatc ctgcctgtga
     1981 gaaaactttt cccggttgcc gccgtttttg catttcttct tttttcatgc aaacccgatg
     2041 atcctgcccc gcagttcacg gacagcacgg acggcagcag ccccggcgtc gatacgttcg
     2101 tcgtcagcag atcgcatgcg ctttccccgg ccgaccagca ggagatcgac aaggacacgc
     2161 aggaaacgcg gcagatcgtg aatgatgcgg tgacgctgat caacggcggg caggcgccgt
     2221 cttcttccaa tgtcaatgac gacgacatcg tgatcgcgcc cgacgatcct cccacttttc
     2281 ccggcgggca ggatgcgatg gatgaataca ttcaccgcaa actcgtgtac ccgctcacgg
     2341 cgatgcagaa cgacataaaa ggaaccgtga cgctgaaagt ggtcatcgaa aaagacgggc
     2401 aggtgggagc gatctcgatc gtgcgcgggc tgggatacgg atgcgacgaa gcggccgtcg
     2461 aatgcgtgcg cgggatgccg aaatggatac ccgcgaaaaa agcaggttac aacgttcgca
     2521 ccacggtcac gatcccgctt tatttcgcga aagacgacga ctgaacgctc ttccaaaaaa
     2581 ggttaatacc cggcgcgcat tccgggagca gaaggaagca ttttacggta atacctatat
     2641 ttgcgcaaac catttttccc gtgaaagaaa aattatcatt ggcgctcaat gccattctca
     2701 tcgtagcggt cattattctt ttctacctgc atttttcgtc gcgcgcagca attgcggatg
     2761 taaaaaaggt gcaggattct tccgccacca gccttacgtt ccgcatcccg aaaaatcttg
     2821 cgggtgcacg cgtgctttac gtgaacatcg attccatttc gacgaagtac aaagcttttg
     2881 ccgacctcag cagttccgaa ggcaccaaga tcgatacgaa gatgcagcag taccagcgca
     2941 gggcgatggc gctccagcag cgtatgaatg acctgaacac caaagcgcag aacaacctga
     3001 tctcgggcga tgatgcgcag aaggaacagg acgcgatcga caaggaacag aaagcgctga
     3061 acgcaatgga gatcgagatc aacgatatgc agaccaaggc gcaggaaaag aaccaggtca
     3121 ttaccgagga ggttttcaaa tatttcaatc agtacagccg cgagaaaaag atcgatttca
     3181 ttctcgctta cggcgcaggc agccctatca tttatgccaa tgacagcctc gatgtgacga
     3241 atgatgtagt ggatgcgctg aacgcgaatt acgacgcgaa ccagaaaaac aatcccatgc
     3301 cgctcgcgcc gaaaggcaac tgacggttga aaccttgttc ataagtaggg gcgcctttct
     3361 tcttgcagat cggcgccccg gttgtttttt tacggaaaca aattaccaat ggaaaaagcg
     3421 acggatcaga acacgctggc cggaaaaatt tcggccgtag aagagtttca
//
LOCUS       DASTSF010000159         2984 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_208620,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000159 DASTSF010000000
VERSION     DASTSF010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2984
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208620"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2411
                     /locus_tag="VFU15_04620"
     CDS             <1..2411
                     /locus_tag="VFU15_04620"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEU4717089.1"
                     /translation="VGGTSMSTYGPSSNILRAISTGTASGDGGIGNANSGGVLGNGGS
                     NADGIAVFTSPVAGITSSTVPVDAIFYGSGIGGALVSAGTAGYQLPVNDLYPGGKLQS
                     TSFFGPDPAPGQYIFATGSYDVQTGSFTTPRTWNTSASFTNLASSVLLQGLYNFSWSN
                     SSTTQNISGLSAGTYTYTISDNSPCPATGTITITEPPVLAVSVATQTDVSCNGANDGM
                     VTVNATGGTPAYAYFWSPSGGTAAASDPLAPGSYTCTVTDSHACTSNASVTITEPAAL
                     TVSASSQTDVGCNGGNNGDASVTASGGTAPYMYSWSSGGTSATESGLAAGTYSCIITD
                     AHACTVTQTFSITEPSALAVTNTTQNDAGCFGGNDGDASVTVSGGSAPYTYAWSSGGT
                     AATESGLAAGTYSCVITDSHSCTVTQTFVIAEPVALSCTATLVNVSCNGGSNGSANVT
                     PAGGTGPYTYAWSSGGTSATESGLSAGTYSCVITDSHSCTLTQTVGITEPAALSATAT
                     QNNVSCNGGNNGTATVSVSGGTASYSYAWSSGGTAATESGLAMGTYTCTITDANTCTA
                     TATVNITEPSALSVTTTQTDVTCNGGNNGTATVSVSGGTASYSYAWSSGGTAATESGL
                     AMGTYTCNITDANSCTTSATVNITEPAAIVFSLGPDTMVCSPVTVNFCAPGGFASYLW
                     NDNSTAQCLNAGTSGCYSVLATDANGCQSSDTICLTVNTCTGIDEAAIQGFDLFPNPN
                     NGRFTIVLGSNQTQATLDIFDGAGQLIGSRIIHQDEEIDLSGYAKGFYFARINGETRK
                     LIIQ"
     gene            complement(2626..2832)
                     /locus_tag="VFU15_04625"
     CDS             complement(2626..2832)
                     /locus_tag="VFU15_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEU4717090.1"
                     /translation="MEKFRIKGEFIELIQLLKVLGIAETGGHAKMIVEDGHVKVNGVL
                     EERKRAKLRPGDKIEVGEDVFMLE"
BASE COUNT          693 a          881 c          760 g          650 t
ORIGIN      
        1 acgtcggcgg aacttcgatg tcgacgtacg gtccttcttc gaatatcctg cgcgcaattt
       61 ctaccggaac tgcttccggt gacggcggga tcggcaacgc caactcaggc ggtgtcctgg
      121 gcaacggcgg atccaacgct gacggtatcg ctgtgttcac atcaccggtt gcgggcatta
      181 cgagctcgac tgtccctgtt gatgcgatat tctatggaag cggcatcggc ggtgcactcg
      241 tttcggcagg aaccgcaggt taccagcttc ctgtgaatga tctttatccc ggcgggaaat
      301 tacagtcgac cagtttcttc ggccccgacc cggctcccgg ccagtacatt tttgcaaccg
      361 ggagctatga tgtgcagacc ggcagcttca cgactccgcg tacatggaac acatcagcat
      421 cattcaccaa tcttgcttct tccgtattgc tccagggact ttataatttc tcctggtcga
      481 attcctcaac cacgcaaaac atatccgggc tttcagcagg aacatacacg tatacgatca
      541 gcgacaattc gccttgtcct gcaacaggaa cgatcacaat tacagaacct cccgttcttg
      601 cagttagtgt cgccacacaa acagatgttt cctgcaacgg cgcgaacgac gggatggtaa
      661 cggtgaatgc gacaggcgga acacctgcat acgcatactt ctggtcgcct tccggcggaa
      721 cagccgccgc ttccgatccc cttgcaccgg gatcttatac ctgcacggtt accgattcgc
      781 acgcgtgcac atcaaacgca agcgtgacca ttaccgaacc ggctgcgctc acagtttccg
      841 cttcttcgca gaccgacgtg ggatgcaacg gtggtaacaa cggcgatgca tctgtaaccg
      901 cttccggcgg aacggctccg tacatgtatt cctggtcttc cggcggaact tctgcaactg
      961 aatcaggact cgctgcggga acctattcct gcatcatcac cgatgcacac gcatgcacgg
     1021 taacacaaac attttccata acggaacctt ctgcacttgc tgttaccaac acaacgcaaa
     1081 acgatgccgg ttgtttcggt ggaaatgacg gagacgcctc tgtaacggtt tccggtggtt
     1141 cggcgccata cacttatgca tggtcgtcag gtggaacagc cgccaccgaa tcgggactcg
     1201 ctgcgggaac atattcgtgc gtcatcacgg attctcattc ctgcacggta acgcagacat
     1261 tcgtcatcgc cgaacccgta gcactttcct gcacggcaac gctggtgaac gtctcctgta
     1321 acggaggcag caacggcagc gcaaacgtaa cgcctgcggg cggaacaggc ccatacacgt
     1381 acgcgtggtc ttccggcgga acttctgcaa ctgaatccgg gctttctgcc ggcacctatt
     1441 cctgcgtcat caccgattca cattcgtgca cgctgacaca gaccgtgggc attaccgaac
     1501 ccgcagcact atccgctacc gcaacgcaga ataatgtttc ctgcaacggc ggaaataacg
     1561 gaacggcaac cgtcagcgtc agcggcggaa ctgcatcgta ttcgtatgcg tggtcttccg
     1621 gcggaacagc ggcaaccgaa tccgggcttg ccatgggaac gtacacatgc actatcacgg
     1681 acgcgaacac ttgcacggca acggcgaccg ttaacatcac cgagccttcc gcgctttccg
     1741 taacaacaac gcagactgac gttacctgca acggcggaaa caacggaacg gcaacggtca
     1801 gcgtcagcgg cggaactgca tcgtattcgt atgcgtggtc ttccggcgga acagcagcaa
     1861 ccgaatccgg gcttgccatg ggaacataca cgtgcaacat cacagatgcg aacagctgca
     1921 cgacaagtgc aacagtcaat atcacggaac cagctgcgat cgtattttcg ctcggacccg
     1981 acacaatggt ttgctcgccg gtgaccgtga atttctgcgc accgggagga ttcgcttctt
     2041 acctgtggaa cgacaacagc acagcgcagt gcctcaacgc aggcacgagc ggatgctaca
     2101 gcgtgctggc gacagatgcg aacggttgcc agtcttcgga cacgatctgt cttacggtga
     2161 atacctgtac gggaatcgat gaagctgcga tacagggatt cgatcttttc ccgaacccga
     2221 acaacggtcg ctttacgatc gtgctcggca gcaatcaaac ccaggcgacg cttgatatct
     2281 tcgatggagc cggccagctc atcggcagca ggatcatcca ccaggatgaa gagatcgatc
     2341 tttccggtta cgcaaaagga ttttactttg cgcgcatcaa tggcgaaaca cgcaagctga
     2401 tcattcaata aagcacgccg caaaaattaa acgtatcgct tgccggtggt tcgggcgata
     2461 gtcgaggcca ctgtcagttc gagcgatagt cgaggccacc gtcagttcga gcgatagtcg
     2521 agaacagttc gagcgatagt cgagaacacc gtcagttcga gcgatagtcg agaacaggtt
     2581 gccttcccag gcccacaaga gtcgtcagca atggcgactc tttttttatt ccagcatgaa
     2641 cacatcttct cccacttcga tcttgtcacc gggacgaagc ttcgcacgtt ttcgttcttc
     2701 caaaactccg ttcaccttca cgtgaccgtc ttccacgatc attttcgcgt gaccgccggt
     2761 ttccgcgatg ccgagaactt tcagcagctg gatcagctcg atgaattctc ctttgatcct
     2821 gaatttttcc ataggtcaga aaaaagggcg gaaaatatcc gcccgtgata ctctatcctg
     2881 aaataagctg tgcacttacg ctttcacgcc cgcacggatg atgttcagcg caccgcccgc
     2941 cttgaaccat tcgatctggc cggcgttgta tgtgtggttc gctt
//
LOCUS       DASTSF010000160        13917 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_126169,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000160 DASTSF010000000
VERSION     DASTSF010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 13917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13917
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126169"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            64..837
                     /locus_tag="VFU15_04630"
     CDS             64..837
                     /locus_tag="VFU15_04630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU4717091.1"
                     /translation="MKKAAFVFLTVLAQLSYACGGGTDAKTVMNKNENSKPVTITFPS
                     LDSLTVTADMYVRNDSLPWMLLCHQAGYSRGEYIETAQFFMDCGYNCLAIDQRSGNEV
                     NGVKNETAALAKKKDLPTDYLSAEQDIVAGLNYLFAKSGRPVVLVGSSYSAGLVLKIA
                     TGNPEVKAVLSFSPGEYYDGKFRVAETVKPLDKPVFITSAKREVPDAKKIFDAIGSTQ
                     KTFFSPTVEGVHASSCLWSSTPGYEEYRDAVKKFLAGIH"
     gene            904..1386
                     /locus_tag="VFU15_04635"
     CDS             904..1386
                     /locus_tag="VFU15_04635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717092.1"
                     /translation="MDCYLVQVGKGAETLHLLTPFSNEELAGNNSRSEFFIGRLVKPE
                     EGVKPENISFNLDFLVAMHQVVREVMAEDPEVTEKAKEQENGFVFIVDRRSPEDSEVE
                     KEDIIGIFLCNERKTDAARYRPNPDYKLISKRGMGMLPMTAEVELKRRILEVPSAGQE
                     "
     gene            complement(1388..2002)
                     /locus_tag="VFU15_04640"
     CDS             complement(1388..2002)
                     /locus_tag="VFU15_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013631976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HEU4717093.1"
                     /translation="MLFDDTYKTVSGPAEGIFKDRGSRFLGFIFPVADEDEVKEKIAA
                     LKKEHFGARHHCYAFRLGPARQHFRSNDDGEPSGTAGKPILGQIQSHDLTNVLIVVVR
                     YFGGTLLGVSGLIAAYRGAAADAIAHADIIEKQVKEIYRLSFPYAAMNEVMKILKDNE
                     LGQWGQDFQLSCTLHFSVRRSNADAVLEKLKRIDDIKCEWEKTE"
     gene            complement(2007..2879)
                     /locus_tag="VFU15_04645"
     CDS             complement(2007..2879)
                     /locus_tag="VFU15_04645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEU4717094.1"
                     /translation="MLWLLLSILTNSALLLILKAFDRFHVNTLQGIVVNYLVAGSLGF
                     ILSGLPLPLGELPHQPWSWVPPVLGVLFISIFFLLARTAQTIGVSVATVANKMSVVIP
                     VFAAILLYHESVTVLKIAGVMIAVVAVYFTSLPKEKTGTSLWRHLLLPVIVFIGSGII
                     DALVNHAKNRLVPDNQLPAFISLSFLTAFCIGVIIITTKIIRGKEKFETKSIAAGIVL
                     GLPNYFSIYGMTRALGSGIMESSALYPVNNMGIVIVSALGALLFFRERLSWKNWLGIF
                     LSLGAIAMIAIHLN"
     gene            complement(3095..4144)
                     /gene="ribD"
                     /locus_tag="VFU15_04650"
     CDS             complement(3095..4144)
                     /gene="ribD"
                     /locus_tag="VFU15_04650"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019989712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEU4717095.1"
                     /translation="MADHEKYMRRCLELAEKGAGRVAPNPMVGCVIVRNDVIIAEGYH
                     MQYGGPHAEVNAIRHVADERALRESTLYVNLEPCAHFGKTPPCADLILEKKIPYVVIG
                     CMDPNPQVQGKGIHRLLQNGVDVKVGVLEKEAQFLNRRFFTGIREQRPYIVLKWAQSA
                     DGFIDHNRDQNNGERAIVSSPEAHLLVHKWRSEEAAIMVGTNTVISDDPQLTVRLTEG
                     PNPVRVTFDRRNRIPHEAKILDGTSPTIIFTEGAHYTTERAEFIPIDFSAHPLRQALA
                     ILHEKKLLSVLVEGGTAILNKFFEENLWDEVRVLVSPKEFGGGVKAPVFPVTDFSTEN
                     VGPDKLYRFYNRDAD"
     gene            4288..4974
                     /locus_tag="VFU15_04655"
     CDS             4288..4974
                     /locus_tag="VFU15_04655"
                     /inference="COORDINATES: protein motif:HMM:NF019542.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717096.1"
                     /translation="MAVYRFRITFEDNDEVYREIDILGKQTFDALHRAIQEAIGFDDS
                     KPASFFMSDDYWRKGQELALFVKKNDDDDDDDYPSRKKSAPKEMSKSKVVEYIDDPHQ
                     KIVYVFDPEVKWTLYLELVKILNDDPKINYPKCTKSAGAAPKQYKQKIAPPPVDEEEE
                     DDEEDKKSKERVFTAEVTYEETEQLGSSEGEEESHGEGGDEEQQAEGEEEGSEESDFS
                     SSFGSDNEDF"
     gene            4974..5873
                     /gene="miaA"
                     /locus_tag="VFU15_04660"
     CDS             4974..5873
                     /gene="miaA"
                     /locus_tag="VFU15_04660"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEU4717097.1"
                     /translation="MQKPLLVVVCGPTGIGKTGISIELAEHFQTCIVSADSRQFYREI
                     PIGTAQPSPGELERVPHFFIASHSIHEPVDAGTYAREVHALLAREFEKRKIVFLTGGS
                     GLFINAVIHGFDELPSQDAELRAALQQTVDENGLGPLLEELESTDPEYFALVDRQNPA
                     RVIRAIEVCRLSGKKYSALCSQKERENFFRVVKLGLEMPREKLYERINKRVDEMLEAG
                     LEEEARRVFPFRHLNALQTVGYKELFDYFEGKTSREKAVELIRQHSRNYAKRQMTWWR
                     KDAEIKWFEPGKITEMMEMINEE"
     gene            complement(5894..6475)
                     /locus_tag="VFU15_04665"
     CDS             complement(5894..6475)
                     /locus_tag="VFU15_04665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717098.1"
                     /translation="MRAALIFFLFLLIAALGIFFFHGRIFPKNPARPSSPGMLPVDSI
                     HHGAWIFFSRDEFPQDEIVNNLGGKNLRTLKGPYVEHKLSPAELLTLDSTLKGLYNVH
                     EARMDPLLDCYRPRHAVVLFDDKGIAYKWIDICFECNGERAMPANFSNATLKAWKIFF
                     TRFGWPVDGDYRTFFTKAQTDSLFRKGKGTFHF"
     gene            complement(6542..7057)
                     /gene="purE"
                     /locus_tag="VFU15_04670"
     CDS             complement(6542..7057)
                     /gene="purE"
                     /locus_tag="VFU15_04670"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010600732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEU4717099.1"
                     /translation="MHPLVGIIMGSASDLKVMNDAAAILDQLEVPFEISVVSAHRTPD
                     RMFEYAKTAASRGLKVIIAGAGGAAHLPGMVASLTPLPVIGVPVKSSNSIDGWDSILS
                     ILQMPNGVPVATVALNAAKNAGILAAQIVSTSDPYLQQRITTYKEELKTKVLESEKET
                     DHVKFKHRKKQ"
     gene            7236..8204
                     /locus_tag="VFU15_04675"
     CDS             7236..8204
                     /locus_tag="VFU15_04675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717100.1"
                     /translation="MSALLEVVISLAFIYLIFSIIVSGIIELWQMFTQRRGAFLYKAL
                     NDIYNDPLNKDFMFLVLSHPLVDRLRETDKTYPHYIGGEIFSDALIDVVRSHYRLPEY
                     TFDKKEKKFLVNMPSLTPQRDGGGKKIVDEFLEGVDALKESDLKQLLRTFAFGVEDYA
                     GLKKNIMSWFSQYMNATSTWYKKGITRALLIVATVITVSFNIDAIHITKQLFRDKRLR
                     DVIVQKADSYVQNKAGRDSVYSSMPDSTMAQNVKKQVTKIRDAYTEAGLLDLPIGWPR
                     PEGQNLFLMFFGWIISVSALGYGADNWFNLLVRLINIRTSVKPKEK"
     gene            8212..9345
                     /locus_tag="VFU15_04680"
     CDS             8212..9345
                     /locus_tag="VFU15_04680"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU4717101.1"
                     /translation="MKYYNAHTHIFTAQYVPNNVVGIPVMNFLSRSPFGRGLADFAGM
                     LTFGKVKGSVERVAKFAEIGLGRSQEVIFNRLQDYYRGWGDVRFVILMMDMEQMGAGQ
                     PLSNYETQLHEIIQLRARGNYRDVLLPFICIDPRRKEFTDGAALLDWVKPFFEKYGFV
                     GIKMYPALGYYPFDEKLDALYGWAQERQVPILYHCIKGVIHYRGPLEGFPPPRYPNPF
                     HDLTIKEQEWYQCNFTEPENYADVLLRFPELKICLAHYGGEDEINGNGRFRGRWYEAI
                     KRLITTPQYRNVYTDVSFSLNDPKIFPKMLSDIQTPLLGERILFGTDYYVVEKDKDEA
                     MLRDELRKFLQVNVTHNGRTMNAFDQIASVNPSKFLTSEFYTA"
     gene            complement(9374..10711)
                     /locus_tag="VFU15_04685"
     CDS             complement(9374..10711)
                     /locus_tag="VFU15_04685"
                     /inference="COORDINATES: protein motif:HMM:NF012861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEU4717102.1"
                     /translation="MNERFEEQTAIFFSILKWIALAVIIGILVGLSTTVFLKLLNISS
                     DFGKSIPYYFVSLPLVFVLSTWIINKLAPNAKGHGTEKVIEAIHRRSGRVPILVVPVK
                     LFTTIITLAAGGSVGKEGPCGQIGAGLASFFSDIFRLKDADRKKLAICGISAGFAAVF
                     GTPISGAIFGLEVLYVGAVMYDVMLPSFISGIIAYQVCTSFGIHYFHSNISFTPAFSQ
                     SFFLKVMLAGIFFGIVSFLFIESFRFTEKKVERWKLNEYARNFIGGSILVGLIFVFST
                     RYCGLGISVIENLLNTGEKAFWYGFLLKILFTIATLAFGGSGGIVTPTFFIGATAGSL
                     FAQIMGLDTATFAAIGMVSLLSGAANTPIAASIMGVELFGGTLAPYAAISCVLAFLMT
                     GHRSIYPSQILSIAKSPTVHVDMGKEMHDVEASVNMKERYTLYRFWKKWRLRK"
     gene            complement(10955..12085)
                     /locus_tag="VFU15_04690"
     CDS             complement(10955..12085)
                     /locus_tag="VFU15_04690"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003011584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEU4717103.1"
                     /translation="MSFGKTLGILGGGQLGRMLIQEAVNWNIDVHILDPDKNAPCRDI
                     ATHFEHGRLDDFETVLAFGKKCGLLTIEIEHVNVDAMEQLEKEGLEVYPQPRVLRMIQ
                     DKGLQKLFYRDNNIPTAPFELVESKQEIAKHASSFPFMQKLRKGGYDGKGVTPLKDPA
                     RLDNAFDAPSVLEKFVDFKKEISVIVARNTKGEIKSFPAVDMLFNPEANLVEFLFSPA
                     DISPALEKEAERIACLVAEKLEITGLLAVEMFVTKDDRILVNEIAPRPHNSGHQTIEG
                     NYSSQYEQHLRAVFGLPLGSTAIVEPSVMVNLLGEKGFEGEAKYEGLEEVLKMEGVYV
                     HLYGKKITKPFRKMGHVTVLGKTVEDALKKARIVKEKLRIIA"
     gene            complement(12554..13573)
                     /locus_tag="VFU15_04695"
     CDS             complement(12554..13573)
                     /locus_tag="VFU15_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009581266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntG family PLP-dependent aldolase"
                     /protein_id="HEU4717104.1"
                     /translation="MTIDLRSDTVTKPTKEMLDAMFSAPVGDDVFNEDPSVNALEEKA
                     AKLFGKEAALFCPSGTMTNQIAINVHTKPGDEVICDYTSHIYQYEGGGIARNSGVQAK
                     LIHGDMGRITAQQVEESINATFDWLTRSKLVSIENTVNRKGGSCYPLAQMKELNALCR
                     KHGLLMHLDGARIFNAITAEGYSTTEIGPLFDSISFCLSKGLGTPVGSMLLGSKEFIR
                     EARRVRKVFGGGMRQAGFLAAAGIYALDHHIDRLKDDHRRAKELADVITALPYVKEVL
                     PVSTNIVIFTLHDAMPLDYFLENIEDKGIRGAIFGKQTIRFVTHLDIDDAMVEKTIKV
                     MKALR"
     gene            13713..>13917
                     /locus_tag="VFU15_04700"
     CDS             13713..>13917
                     /locus_tag="VFU15_04700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717105.1"
                     /translation="MRKTLFVVSFFFAFIAPSFSQTAWWVFFTDKNGTTFDPYAYFAP
                     EAIDRRNRLGISLYDSTDFPVQQK"
BASE COUNT         3300 a         3509 c         3918 g         3190 t
ORIGIN      
        1 gcaggtgtac gggcacctgc cggaagggga aactgttttt cccggactta tcgccgaaca
       61 aaaatgaaaa aagcagcttt tgtttttctc accgttcttg cacaactctc gtatgcctgc
      121 ggaggcggaa ccgacgccaa aaccgtcatg aacaaaaacg aaaacagcaa gcccgtgacg
      181 atcacgtttc catcgctcga cagcttaacc gtcactgcgg acatgtacgt cagaaacgat
      241 tcgcttccct ggatgctgct ctgccaccag gccggttaca gcaggggcga gtacatcgag
      301 acggcgcagt ttttcatgga ctgcggctac aactgcctcg cgatcgacca gcgttccggg
      361 aacgaagtga acggcgtgaa gaatgaaacg gccgcgctgg cgaagaagaa agatcttccc
      421 acggattacc tgagcgcgga gcaggatatt gtcgccgggc tgaattatct tttcgcgaaa
      481 agcggccggc cggtcgtgct ggtcggcagc tcgtattccg cgggactcgt gctgaagatc
      541 gcaacaggca acccggaggt gaaagccgtg ctctcgtttt ctccgggcga atattacgac
      601 ggtaaattcc gcgtagcgga aaccgtgaag ccgctggaca agcccgtgtt catcacatcc
      661 gcgaaacgtg aagtgccgga tgcgaaaaaa atattcgacg cgatcggctc gacgcagaaa
      721 acgtttttct cacccaccgt cgaaggcgtt cacgcttcga gctgcctgtg gagcagcacg
      781 ccgggctacg aagaataccg tgacgccgta aaaaaattcc tggcgggaat tcattgagcc
      841 cattttctaa agggagaaag ttcccggttc cattttcagg acctgcgacc ttatctttgc
      901 acaatggatt gttatctcgt acaagtcggc aaaggcgcgg aaacactgca tttgctgacg
      961 cctttttcga acgaagagct cgccggaaat aattcgcggt cggaattttt catcgggcgt
     1021 ttggtgaaac cggaagaagg cgtgaagccg gaaaacattt cgttcaatct cgattttctc
     1081 gttgcgatgc accaggttgt gcgggaagtg atggcggaag atccggaagt gacggaaaaa
     1141 gcgaaagagc aggagaacgg ttttgttttt atcgttgaca ggcggtcacc ggaggacagt
     1201 gaagtggaaa aggaagacat catcggtatt tttttgtgca acgaaagaaa aacggacgcc
     1261 gcgcgttacc ggccgaaccc ggattacaag ctcatctcga aacgcggcat gggaatgctg
     1321 cccatgacgg cggaagttga gctgaagaga agaatccttg aggtgccgtc agccgggcag
     1381 gaatgattta ttctgttttt tcccactcgc attttatatc atcaatccgt ttcagctttt
     1441 ccagcacggc atccgcattc gatctccgca cggaaaaatg caatgtgcac gagagctgga
     1501 aatcctgtcc ccattgcccg agctcattgt ccttgagaat tttcatcacc tcgttcatcg
     1561 cggcataggg aaaggaaagc cggtaaattt ccttcacctg tttttcgatt atgtctgcat
     1621 gcgcgatcgc atctgccgct gcaccgcggt aagccgcgat gagcccgctg acgccgagca
     1681 gtgttccgcc gaaataacgc accacgacga tcagcacgtt cgtcaggtcg tgcgactgga
     1741 tctggccgag gataggtttc ccggcggtgc ctgaaggttc gccgtcgtcg ttcgaacgga
     1801 aatgctgcct ggcgggaccg agacggaatg catagcaatg atggcgcgcg ccgaaatgtt
     1861 ctttcttcag cgcggcaatt ttttccttca cttcatcttc atccgcaacc gggaaaataa
     1921 atccgagaaa acggctgccg cggtctttga aaattccttc agcgggaccg gaaacggttt
     1981 tgtatgtgtc gtcgaatagc aaaacgtcag ttgaggtgaa tagcgatcat cgcgatggcg
     2041 ccgagtgaaa ggaaaatgcc cagccagttt ttccaggata atcgttcgcg aaaaaaaagc
     2101 agcgcgccca atgcggaaac gatgacgatg cccatgttgt tcaccgggta aagtgcagag
     2161 ctttccatga tgccgctgcc gagcgcacgc gtcatcccgt aaatggaaaa atagttcggt
     2221 aagccgagaa cgatacccgc agctatgctt ttcgtttcga acttttcttt tccgcggatg
     2281 atcttcgtcg tgataatgat cacgccgatg cagaaagcgg tgaggaagga aagcgaaata
     2341 aaagcaggaa gctggttgtc cggcacgagc ctgttcttcg catggttcac cagcgcgtcg
     2401 atgataccgc tgccgatgaa aacgatcacc ggtaaaagca aatgccgcca cagggacgtt
     2461 cccgtttttt ccttcggaag ggaagtgaag taaacggcga ctacggcgat catcacaccg
     2521 gcgatcttaa gaacagtgac ggattcatgg taaagcagga tcgctgcaaa aacagggatc
     2581 accactgaca ttttattcgc caccgtcgct acggaaacgc cgatggtctg cgcagtgcgt
     2641 gcgagaagaa aaaagatcga gatgaaaagc acgccgagca cgggcggaac ccagctccag
     2701 ggctgatgcg gcaattcgcc gagcggaagc ggaagcccgg acaaaataaa accaagcgag
     2761 ccggcaacga gataattcac gacaattccc tgcagcgtgt tcacgtgaaa acggtcgaac
     2821 gctttcagga tgagcagcag cgcgctgttg gtgagaatgc tgagtaacag ccaaagcata
     2881 ggaaagggag tttcgggttt ccgagttaac agttttcagt tcccggttcg ctgttcgtcg
     2941 ttccggcttt gagacccgga acctggtttg tttttagttt gtcatgcgtc atgcgccgtt
     3001 ctttgtgttt ttggtgaaaa acggttgtgt tcttcatgat gaaaaattcg acggtgttct
     3061 tttttcggtg atccggaatt ttctgcctgg aatttcagtc cgcatcacga ttgtaaaacc
     3121 tgtacaattt atccggacca acattttccg tgctgaaatc agtgaccggg aaaacgggcg
     3181 cttttacacc gccgccgaat tctttcgggg aaacgagcac ccggacttca tcccacaggt
     3241 tttcctcgaa aaatttattc agtatcgcgg tgccgccttc gacgagaacc gagagcagtt
     3301 ttttttcatg gaggatcgcg agcgcctggc gcaggggatg tgcagagaaa tcgatcggga
     3361 tgaattcagc gcgttcggtg gtgtaatgcg cgccctcggt gaaaatgatc gtcggggaag
     3421 tgccgtcgag gatcttcgcc tcgtgcggga tgcggtttcg ccggtcgaac gtgacgcgca
     3481 ccggattcgg tccttccgtc aggcgcacgg tgagctgcgg atcgtcggaa ataacggtgt
     3541 tcgtgccgac catgatcgcg gcttcctccg aacgccattt atgcacgagc aggtgtgctt
     3601 ccggcgagct gacgatcgcg cgttccccgt tgttctggtc gcggttgtga tcgatgaacc
     3661 cgtcggccga ctgtgcccat ttgagcacga tgtagggccg ctgttcgcgg atgccggtga
     3721 agaaccgccg gttgaggaac tgcgcttctt tttccagcac accgactttc acgtcgacgc
     3781 cgttctgcag cagccggtga attccttttc cctgcacctg cggattggga tccatgcagc
     3841 cgatcaccac ataagggatc ttcttttcga ggatgaggtc ggcgcagggc ggtgtttttc
     3901 cgaaatgggc gcacggctcg agattcacgt acagcgtcga ttcgcggagt gcacgttcat
     3961 cggccacatg gcggatggca ttcacttcgg cgtgaggtcc gccgtattgc atgtggtaac
     4021 cttccgctat gatcacatcg ttgcgcacga tcacgcaccc gaccataggg ttgggcgcca
     4081 cccggccggc ccctttttcg gccagttcga ggcatcgcct catgtatttt tcgtggtcag
     4141 ccacggcgca aatataacgg gaaggggccc cgctttcatc tgttcccgga acgttctggt
     4201 caccctgtaa acggtatttg tccggcgccc cctgcgtgct gtcgaacggg tttgattttt
     4261 atctttcata ttcgataaat tcgcaccatg gctgtttacc gttttcgcat aacttttgaa
     4321 gataacgatg aagtttaccg ggagatcgat attctcggca agcagacatt cgacgcgttg
     4381 caccgcgcca tccaggaagc gatcgggttt gacgattcaa aaccggcgtc gttctttatg
     4441 agcgacgatt actggaggaa agggcaggag cttgcgcttt ttgtgaagaa gaacgatgat
     4501 gacgacgatg atgactatcc ttcacggaaa aaatccgctc cgaaggaaat gtcgaaaagc
     4561 aaagtcgtgg aatacatcga tgatccgcac cagaagatcg tgtatgtgtt cgatcctgaa
     4621 gtcaaatgga cgctttacct cgagctggtg aagatcctca atgacgatcc gaagatcaat
     4681 tacccgaaat gcacgaaatc ggccggcgct gcaccgaaac aatacaaaca gaagatcgcg
     4741 cctcctcctg tggatgaaga agaagaggac gacgaggaag ataaaaagtc aaaagagcgc
     4801 gtattcacgg ccgaggtcac ttacgaagaa accgaacagc tcgggtcttc cgagggcgaa
     4861 gaagaaagcc atggcgaagg cggcgatgaa gagcagcagg cagagggtga ggaagaaggc
     4921 agcgaggaaa gcgatttcag ctcttctttc ggaagcgata acgaagattt ctgatgcaga
     4981 agccgctcct ggttgtcgtg tgcggtccca cggggatcgg taagaccggg atctccatcg
     5041 agctggcaga acattttcag acgtgcattg tttctgcgga ctcacggcag ttttacaggg
     5101 aaattcctat cggcaccgcg cagccttcac cgggtgagtt ggaacgcgtt ccgcattttt
     5161 tcatcgcttc gcattccata cacgaaccgg tcgatgccgg aacatacgcg cgcgaagtgc
     5221 atgcgctgct ggcgcgtgaa tttgaaaaaa ggaagatcgt tttcctcacg ggcggcagcg
     5281 ggcttttcat caatgcggtc attcatggtt tcgacgagct cccgtcgcag gatgcggaac
     5341 tgcgggcagc gctgcagcaa acggtggacg agaacggtct aggtccgctg ctggaagagc
     5401 tggaaagcac cgatcccgaa tattttgcgc tggtagacag gcaaaatccc gcgcgtgtga
     5461 tccgcgcgat cgaggtgtgc aggctgtcgg gaaaaaaata ttcggcgctg tgttcgcaga
     5521 aagagaggga gaattttttc cgcgtggtga agctgggact cgaaatgccg cgggaaaaat
     5581 tatacgaacg gatcaataag cgtgtggatg aaatgctgga agcgggactg gaagaagaag
     5641 cgagaagagt attcccgttt cgtcacctca atgcgctgca aacggtgggc tacaaggagc
     5701 tgttcgatta tttcgaagga aaaacaagcc gtgaaaaagc ggtggagctg atcaggcagc
     5761 attcgcgcaa ttacgccaag cggcagatga catggtggag aaaagatgcg gagataaaat
     5821 ggtttgagcc gggaaagatc acggagatga tggaaatgat aaatgaggaa tgattgcggc
     5881 tggttggccg ttctcagaag tgaaacgtcc cttttccttt ccggaacaaa ctgtcggttt
     5941 gggctttggt gaagaacgtt ctgtaatcgc cgtcgacagg ccagccgaaa cgggtgaaga
     6001 aaattttcca ggcttttaat gtcgcgttcg agaagttcgc gggcatggcg cgctccccgt
     6061 tgcattcgaa gcagatgtcg atccacttgt aagcgattcc tttatcatca aacaaaacca
     6121 ccgcatgccg cgggcgatag caatcaagca gcggatccat gcgcgcttcg tggacattgt
     6181 acaatccttt cagcgtgctg tcgagcgtga gaagctcggc cggggaaagc ttatgttcca
     6241 catacggacc tttgagtgtg cgcaggtttt tcccgccaag gttgttgacg atctcgtcct
     6301 gcggaaattc atcgcgcgag aaaaatatcc aggctccgtg gtggatcgaa tcgaccggaa
     6361 gcataccggg tgaagaaggg cgtgcaggat ttttgggaaa gatgcgcccg tggaaaaaga
     6421 aaataccgag cgctgcaatc agcaggaaca aaaagaagat cagcgcggct ctcatcgggc
     6481 gggtgcggtt ttccggtttc gtgtccgata ctcagcgttc ggaacccgga acgtttatac
     6541 tttactgttt tttccggtgc ttgaatttca cgtggtcggt ttctttttcg ctttcgagca
     6601 cttttgtttt caattcctcc ttgtacgtgg taatgcgctg ctgcaggtaa ggatctgaag
     6661 tggaaacgat ctgcgcggcg agaatgcccg cattttttgc agcgttgagc gcaaccgtgg
     6721 ccacaggaac gccgttcggc atctgcagga tggaaaggat ggaatcccag ccgtcgatgg
     6781 agttggacga tttcaccggg acaccgatca cgggcagggg cgtcagcgaa gcgaccatcc
     6841 cgggaagatg cgccgcacct ccggcaccgg cgatgatgac tttcagtccg cgggaagcag
     6901 cggtctttgc atattcgaac atgcggtcgg gcgtacggtg tgcggagacg acggaaattt
     6961 caaacgggac ctcgagctgg tcgaggatgg cggctgcatc gttcatgacc ttcaggtcgg
     7021 atgcgctgcc catgataatg cctacaagtg gatgcatgac gtaaagataa gttatcggga
     7081 atagtaaaca gcaacgggtc aacagtgaaa aggatgcagg cgggcaggga agaaacagcg
     7141 caattttcag ggggcggttt atccgttgcg accggtccct gttcaattat tattgtattt
     7201 tcactttgaa atcgtttcaa tagttcatcc cgcccatgag cgcattgctt gaagtcgtca
     7261 tttccctcgc tttcatttac cttatttttt ccatcatcgt ttcggggatc atcgagctgt
     7321 ggcagatgtt cacgcagcgc cgcggcgctt ttctttacaa ggcgctgaat gatatttaca
     7381 acgatccgct gaacaaggat ttcatgttcc tggtcctttc ccatccgctg gtcgaccgcc
     7441 tgcgggaaac cgataaaaca tacccgcatt acatcggcgg ggaaattttt tcggatgcgc
     7501 tgatcgatgt tgtacgcagc cattatcgtt tgccggaata tacgttcgac aaaaaagaga
     7561 agaagttcct tgtgaacatg ccttcgctta cgccgcaacg cgacggaggc ggaaaaaaga
     7621 tcgtggatga attcctggaa ggcgtggacg cgctgaagga aagcgacctc aagcagctgc
     7681 tgcggacatt tgcgttcggt gtggaggatt atgcgggact gaaaaaaaac atcatgagct
     7741 ggttctcgca gtacatgaac gccaccagca cgtggtacaa aaaaggaatt acccgcgcat
     7801 tgctgatcgt ggcgacggtg atcacggttt cgttcaatat cgatgcgatc cacatcacga
     7861 aacaattgtt ccgcgacaag cgcctgcgcg atgttatcgt gcagaaagcg gattcttacg
     7921 tgcagaacaa agccgggcgc gattccgttt acagcagcat gcccgacagc accatggcgc
     7981 agaacgtgaa aaaacaggtg acgaaaataa gggacgccta caccgaagcg ggcctgcttg
     8041 atcttcccat cgggtggccg cgtcccgaag gacagaacct gttccttatg tttttcggct
     8101 ggatcatttc cgtttccgcg ctcggatatg gcgccgacaa ctggttcaac ctgctcgtgc
     8161 gtctcatcaa catacgcacg tcggtcaagc ccaaagaaaa atagtcccgg catgaaatac
     8221 tacaacgcac atacgcacat tttcaccgcg caatacgttc ccaataacgt cgttggaatt
     8281 cctgtcatga atttcctttc gcgttccccg ttcggtcgcg gtcttgcgga tttcgccggc
     8341 atgcttacgt tcggaaaagt gaaaggaagc gtggaacggg tagcgaaatt tgccgagatc
     8401 ggcctgggaa gaagccagga agtgatcttc aaccggctgc aggattatta ccgcggctgg
     8461 ggcgatgtgc gtttcgtgat cctgatgatg gacatggagc agatgggcgc gggacagccg
     8521 ctgagcaatt atgaaacgca gctgcatgag atcatccagc tccgcgcacg cggaaattac
     8581 cgcgacgtgc ttctcccgtt catctgcatc gatccgcgaa ggaaagaatt caccgatggc
     8641 gctgcgctgc tcgactgggt gaagccgttc ttcgaaaaat acggtttcgt cggcataaaa
     8701 atgtatcccg cgctgggcta ttacccgttc gatgaaaagc tcgatgcgtt atacggctgg
     8761 gcgcaggaac ggcaggtccc catcctgtat cattgcatca aaggtgtgat ccattaccgt
     8821 ggtccgctcg aaggttttcc gccgccgcgg tacccgaacc cgttccatga tctcacgatc
     8881 aaagagcagg aatggtacca gtgcaatttc acggaaccgg aaaattatgc ggatgtgctc
     8941 ctgcgttttc ccgagctgaa gatctgcctc gcgcattacg gcggagaaga cgagatcaac
     9001 gggaacgggc gtttcagggg aagatggtac gaagcgatca aacgccttat caccacgccg
     9061 caatacagga acgtgtatac ggatgtttcc ttctcgctga acgatccgaa gatatttccg
     9121 aaaatgcttt ccgatataca gacgccgttg ctcggcgaac gcatcctctt cggtacggat
     9181 tattacgtgg tggaaaaaga caaggatgaa gcgatgctgc gcgacgagct gcggaaattc
     9241 ctccaggtga acgtcacaca caatggaaga acgatgaacg ccttcgacca gatcgcttcc
     9301 gtgaatcctt cgaaattcct gacctcggaa ttttacacgg cctgagcagc cggtgaaata
     9361 cactgcggca aaattatttc cgcagcctcc actttttcca gaaccggtag agtgtatagc
     9421 gttccttcat gttcacggac gcttccacgt cgtgcatttc cttgcccatg tcgacgtgaa
     9481 cggtgggcga tttggcaatg gaaaggatct gcgacggata aatactccgg tggccggtca
     9541 tgaggaaagc gagcacgcag ctgatggcgg cataaggggc aagcgttccg ccgaacagct
     9601 caacgcccat gatgctggcc gcgatcggtg tgttggctgc gccggaaaga aggctcacca
     9661 tcccgatggc cgcgaacgtg gccgtatcga gtcccatgat ctgcgcgaaa agacttccgg
     9721 ccgttgcgcc gataaaaaat gtcggcgtga caatgcctcc gcttccgccg aaggcgagcg
     9781 tggcgatcgt gaaaagtatc ttgagcagga acccgtacca gaacgctttt tcaccggtgt
     9841 tgagcaggtt ctcgatcacg ctgatgccga gtccgcagta acgcgttgaa aaaacaaaaa
     9901 tcaaaccgac gagtatcgag ccgccgataa agttgcgcgc gtattcgttc agcttccagc
     9961 gttccacttt tttctcggtg aagcggaagg attcgatgaa caggaaagag acgatgccga
    10021 agaaaattcc ggcgagcatc accttcagga aaaaagattg tgagaaagcc ggcgtgaacg
    10081 aaatgttgct gtggaaataa tgaatgccga acgacgtgca cacctggtac gcgatgatcc
    10141 cggaaatgaa cgaaggcagc atcacgtcgt acatcacggc gccgacgtac agcacttcga
    10201 gaccgaagat cgcgcccgag atcggcgtgc cgaacacagc agcgaaaccc gcgctgatcc
    10261 cgcagatcgc gagctttttc cggtcggcgt ccttgaggcg gaaaatgtcg gagaagaaag
    10321 aagccagccc ggcgccgatc tgtccgcagg gaccttcctt tcccaccgag ccgcctgcag
    10381 cgagcgtgat gatcgtcgtg aacagcttca cgggaacaac gaggataggc acgcgtccgg
    10441 aacggcggtg aatggcttcg atgacttttt ccgtgccgtg tcccttcgca ttcggtgcca
    10501 gcttgttgat gatccacgtc gacagcacga aaacgagcgg cagcgaaacg aaatagtaag
    10561 gaatggattt gccgaagtca gaggaaatgt tcagcaattt caggaagacc gtggtggaaa
    10621 gtcctacaag tatgccgata ataaccgcga gagcaatcca tttgagaatg ctgaaaaata
    10681 tcgcggtctg ttcttcaaaa cgctcgttca tcactgcaga cgttgcgaag cagccggcag
    10741 ggcgcttaag ttttgtttct gaaaccctgg ggcgggatca ccggacttgc tttcccggcc
    10801 tgttgatcgg ccggatgaat tcactggaaa acggggacaa agttacgcag ttaaacagga
    10861 gaatttacat cggcaggaga taaaacggag aattgattat tgtcaatttt ttatagcaca
    10921 aacaggtaaa cggaacaaag cttcaggcat cctgtcaggc gatgatgcgc agcttttctt
    10981 tcacgatgcg cgcttttttc agtgcgtctt ccactgtttt accgagcacc gtcacgtgtc
    11041 ccatcttgcg gaacggcttc gtgatcttct tcccgtacag gtgaacatac acgccttcca
    11101 tcttcagcac ttcttccagt ccttcgtatt tcgcttctcc ttcgaaccct ttttcaccga
    11161 gcaggttcac catcacggaa ggctcgacga tggcggtgct gccgagcggg agaccgaaaa
    11221 ctgcacgcag gtgctgttcg tattgcgacg agtaatttcc ttcgatggtc tggtggccgc
    11281 tgttgtgcgg acgcggcgca atttcattaa caaggatgcg gtcgtctttc gtcacgaaca
    11341 tttccacggc gagcagccct gtgatctcca gcttttccgc cacgaggcag gcgatgcgtt
    11401 ccgcttcttt ttccagcgca ggtgaaatgt ccgcgggaga aaaaaggaat tcgacgaggt
    11461 tggcttcggg gttgaacagc atgtcgaccg cggggaagct ttttatctcg cctttcgtgt
    11521 tgcgcgcaac gatgacggaa atttcctttt tgaaatcgac gaacttctcg agcacgctcg
    11581 gtgcatcgaa cgcgttgtcc agccgtgcgg ggtctttcag cggtgtgacg cctttgccgt
    11641 cgtagcctcc tttgcgcagc ttctgcatga atggaaaaga tgacgcgtgc ttcgcgatct
    11701 cctgtttgct ttcgacgagc tcgaacggcg cggtggggat gttgttgtcg cggtaaaata
    11761 atttctgcag gcctttgtcc tggatcatgc gcaggacgcg cggctgcggg tagacttcga
    11821 gtccttcctt ttcgagctgt tccatcgcgt ccacgttcac gtgctcgatc tcgatcgtga
    11881 gcagtccgca tttttttccg aaagcgagga cggtttcaaa atcgtcgagg cgaccgtgtt
    11941 cgaaatgcgt ggcgatatcg cggcaggggg cgtttttatc cgggtcaaga atgtgaacgt
    12001 cgatgttcca gttcaccgct tcctggatga gcattctgcc gagctggccg ccgccgagta
    12061 taccgagtgt tttgccgaat gacattttag ctgaggaaaa gtttgttttt taatacggag
    12121 aacgtgaagg ccgtttcacg gttgacacaa agaaaaggaa agcggatgtt ttttaacacg
    12181 gagatcacga aggtcgtttc acggttgacg caaagaaaag gaaagcggat gttttttaac
    12241 acggagatca cagaggtcgt ttcacggagt acacaaagaa aaggaaggtg gatgttttct
    12301 aacacagaga tcacagaggt cgtttcacgg aggacacaaa gaaaaggaaa gcggatattt
    12361 tttgacacgg agcgcacgaa ggtcgtttca cggagtacac aaagaaaagg aaagcggatg
    12421 ttttctgaca cagagatcac agaggttgtt tcacggagat aaagaaaacg aaaccgtttg
    12481 ttctgcattc ggtgttgacc gccgtgtgtt tttctgaatg acattttatg aaccgggact
    12541 gacagaggat agatcagcgc agcgctttca tcaccttgat cgttttctcc accatcgcat
    12601 cgtcgatatc gaggtgcgtg acgaagcgga tcgtctgctt gccgaagatc gcgccccgga
    12661 tgcccttgtc ttcgatgttc tcgaggaaat aatccagcgg catcgcgtcg tgcagggtaa
    12721 agatgacaat gttcgtcgac acgggcagca cttccttcac atacggcaaa gcggtgatca
    12781 catcggcaag ctctttggca cggcggtgat cgtctttcag ccggtcgatg tggtggtcga
    12841 gcgcataaat tcctgccgcg gcgagaaacc cggcctggcg catcccgccg ccaaagactt
    12901 tccgcacgcg ccgcgcttcg cggatgaatt ccttcgagcc gagcagcatc gatcccaccg
    12961 gtgtcccgag tcctttcgaa agacagaatg aaatggaatc gaacagcgga ccgatctccg
    13021 tagtggaata accttccgcg gtgatcgcgt tgaagatgcg cgcgccgtcg aggtgcatga
    13081 gcagcccgtg cttccggcag agcgcgttca gctctttcat ctgtgcaagc ggatagcagc
    13141 tgccgccttt gcggttcact gtattctcga tgctcacgag cttgctgcgc gtcagccagt
    13201 cgaacgtggc gttgatgctt tcctccacct gctgcgcggt gatgcgcccc atatcgccgt
    13261 ggatcagctt ggcctgcacg ccggaattcc gcgcgatgcc gccgccttcg tactggtaaa
    13321 tgtgcgaggt gtaatcgcag atcacttcgt cgccgggctt cgtatgcacg ttgatggcga
    13381 tctggttggt catcgttccc gaaggacaga acagcgcggc ttccttgccg aacagcttcg
    13441 ccgctttttc ctcgagcgcg ttgacggaag gatcttcatt gaacacgtcg tcgcccacgg
    13501 gtgcgctgaa catcgcgtcg agcatttctt tcgtgggttt cgtgacggtg tcgctgcgga
    13561 ggtcgatggt catgtggaat gtattttcgg ttagcgaagg agcaaaggtc ggaagaatag
    13621 atcaaatctt ttgcacatgc agtcccccgt tcgcgacccg gcaacccgtc tgcccctgaa
    13681 ttttttgcat ctttgtttct ctccatccac tgatgcgaaa aacgcttttt gtcgtttctt
    13741 ttttcttcgc tttcatcgcc ccgtcgttct cacagacggc gtggtgggtt tttttcaccg
    13801 acaagaacgg aacgacgttc gatccgtatg cttatttcgc gccggaagcg atcgaccgca
    13861 ggaacaggct cggcatttcc ctgtatgatt ccaccgattt tcccgtgcag cagaaat
//
LOCUS       DASTSF010000161         3223 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1306348,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000161 DASTSF010000000
VERSION     DASTSF010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3223
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1306348"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..874
                     /locus_tag="VFU15_04705"
     CDS             <1..874
                     /locus_tag="VFU15_04705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717106.1"
                     /translation="TEYVKKGENVMLELSSISDASHSSRIRFFRNLAAINYHSAAHDF
                     EKAEKFAGQLLSSVEKDRIVLSRSNRAGANMELANVYLNMGKYDRAVEYATKAAGLFK
                     TGMINQLNAYILLFFSYFRNKQFSEAEDVLRMSQNHRLVKHRINPMMHSRVHLLRAAF
                     TFFQGDVEQSSRILNRNTEPDRDKDGWMPGFYMLDLLILMEKKAVELASYRLEAFRKM
                     IRRYQVCQREMRLPVITSILKDIVKNDFDFRNVLPAHEPEIKKLSEAREQYYWNPAGY
                     EVIRFDEWLMRKVS"
     gene            899..1564
                     /locus_tag="VFU15_04710"
     CDS             899..1564
                     /locus_tag="VFU15_04710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717107.1"
                     /translation="MCNCNKGSSVVKKDTLRKGKAIFPAPNSPAPKNAAALSAKTADV
                     LTLPGETLADRCMTFAYMLGLDEPVSERVLVAALENPGYARRLLSSRDNEKILYDLLN
                     NPPLSGSRGAQFSNGALITKAGKSLMKWALSGFPTVSKETLKRREDACLACPNLREPA
                     NLLQRFTAPAERGATAGTRTGNAICSSCGCVVRNKMRLSTESCPETISGNSEMTRWGE
                     VVK"
     gene            1606..3042
                     /locus_tag="VFU15_04715"
     CDS             1606..3042
                     /locus_tag="VFU15_04715"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU4717108.1"
                     /translation="MNNDMQTEKAAADVSRNISFIVRELGWFSARIRMVLGIDPDQEF
                     SLQNMPPADEKTHYGKFLTGWKADEMERMAIGLCFAQLYKPGTLGGFLALLQNPGHRQ
                     DVGGLFKEGSGRFYLTVRTLAFVMSGRDQEKYGYYSSSLTRRHRLFAEGILLTENPLG
                     ENSFLDYAVYLNDIYYATLLHGSLPLLDGESGFPAVQSKATHTLEDVVLDEETRKEIG
                     EIHTFAEQMNELWALPDASMYRGNCVCIFTGEPGTGKSHSAVALGNEFGMPVYLVNTA
                     QLVSKYIGETEKNLEKVLDRFDNKNCILFFDEAESIFSKRTEVKDAHDRYANQEQSFL
                     LWKMTKFRGIIILATNVQDIRQYFDKAFLRRFYRIINIPFPNYPERKKLWEKSLGSSF
                     RYEDGLTERLAKDFQLTGGSIYNIVSDGVIKALKEKTGVITYELLANALRSEFTKTGR
                     KFEICTDEMVYQNPVRRFGPGYESRTMI"
BASE COUNT          814 a          845 c          899 g          665 t
ORIGIN      
        1 gacggaatac gtgaagaagg gcgaaaatgt gatgctggag ctttcctcta tttccgacgc
       61 ttcgcattcc tcgcgcatcc gtttcttccg caacctcgct gcgatcaatt accattctgc
      121 ggcgcatgat ttcgaaaagg cggagaaatt cgccgggcag ctgctctcgt cggttgagaa
      181 agaccgcatc gttctttccc ggtcgaacag ggcaggagca aacatggagc tggccaacgt
      241 ttacctcaac atgggaaaat acgaccgcgc ggtagaatat gcgaccaaag cggcgggact
      301 gttcaagacc ggaatgatca accagctgaa cgcgtacatt cttctctttt tttcctattt
      361 ccgcaacaag cagttttccg aagcggagga cgtgctgcgc atgtcgcaga accaccggct
      421 cgtgaagcac aggatcaacc cgatgatgca ttcgcgtgtt cacctgctgc gcgctgcgtt
      481 cacgttcttc cagggcgatg tcgaacagag ctcgcgcatt ctcaaccgca acacggaacc
      541 cgatcgcgac aaggacgggt ggatgcccgg cttctacatg ctcgacctgc tcatcctgat
      601 ggaaaagaaa gcggtggagc ttgcttccta ccgcctggag gcgttccgca aaatgatccg
      661 gcgttaccag gtatgccagc gggaaatgcg cctgcctgtc atcaccagta ttttgaaaga
      721 catcgtgaag aacgatttcg atttccggaa tgtgcttcct gcgcatgaac ctgaaataaa
      781 aaaactgtcc gaagcgcgcg aacagtatta ctggaatccc gccggttacg aagtgatccg
      841 cttcgatgaa tggctgatgc ggaaagtttc ctgaagctga aaaaacgatt cgtccgttat
      901 gtgcaactgc aacaaaggtt cctccgtcgt caaaaaagat acgctccgca aagggaaagc
      961 gatttttccc gccccgaaca gcccggctcc gaaaaacgcg gctgctcttt cggcaaaaac
     1021 cgcggatgtc cttacgcttc ccggtgaaac gctggccgac cggtgcatga catttgccta
     1081 catgcttggg ctcgatgaac ccgtctctga gcgtgtgctc gtcgcggcgc tggaaaatcc
     1141 cggttacgca cggcgactgc tttcttcgcg cgacaatgaa aaaatcctgt acgacctgct
     1201 caacaatccc ccgctcagcg ggagccgcgg cgcccagttt tccaatggcg cgctgatcac
     1261 gaaagcggga aagtcgctga tgaaatgggc gctttcgggt tttcccaccg tgtcgaaaga
     1321 aacgctgaaa cggagggaag atgcatgcct cgcctgcccg aatctccggg aaccagcgaa
     1381 tcttttgcag cgtttcaccg cacctgcgga gcgtggcgcg accgcgggca cgcgtaccgg
     1441 aaatgcgatc tgctcttcct gcggttgcgt ggtgcgcaac aagatgaggc tttcgacgga
     1501 atcatgtccc gaaacgatct cgggaaacag tgaaatgacg cggtggggag aagtggtgaa
     1561 gtgatgtgtt gctaatggtt gagcgcggcc ggtaaaacaa caggtatgaa caacgacatg
     1621 caaacggaaa aagcagccgc ggacgtttcc cggaacatct catttatcgt ccgggagctc
     1681 ggatggtttt ctgcacgcat ccggatggtg ctcgggatcg acccggacca ggagttttcg
     1741 ctccagaaca tgcctcccgc ggatgaaaaa acgcattacg gaaaatttct caccgggtgg
     1801 aaggcagatg aaatggaacg gatggccatc ggtctttgtt tcgcgcagct gtacaagccc
     1861 ggaacgctcg gcggatttct ggcgctgctg caaaaccccg gtcaccggca ggatgtgggc
     1921 ggactgttca aggagggaag cggaaggttt tacctcacag tgcgcacact ggcgttcgtc
     1981 atgtcgggca gggaccagga aaaatacggc tactacagca gcagtcttac acgcaggcac
     2041 cgcctgttcg ccgaagggat cctgctgacg gaaaatccgc tgggagaaaa ttcatttctc
     2101 gattacgcgg tttacctcaa tgatatttat tacgccacgc tgctgcacgg atcgcttccg
     2161 ctgctggatg gtgaatcggg ctttcccgcc gtgcagagca aagcgacgca tacgctggaa
     2221 gatgtcgtgc tcgatgaaga aacgaggaaa gagatcggcg agatccatac gttcgctgaa
     2281 cagatgaacg agctgtgggc tttgcccgat gccagtatgt accgcggcaa ttgcgtgtgc
     2341 atttttacgg gagagccggg cacgggaaaa agtcattccg ccgttgcgct cgggaacgag
     2401 ttcggtatgc cggtgtatct cgtgaacacg gcacagctcg tttcgaaata catcggtgaa
     2461 acggaaaaga acctcgagaa agtcctcgac cggtttgaca acaagaactg catcctgttt
     2521 ttcgacgagg cggaatccat tttcagcaag cggacggagg tgaaggacgc gcacgaccgt
     2581 tacgcgaacc aggagcaatc attcctgctc tggaaaatga cgaagttccg cggcatcatc
     2641 attctcgcca cgaacgtgca ggacatacgg cagtatttcg acaaagcgtt cctgcgcagg
     2701 ttctaccgta tcatcaatat tccattcccg aactatcccg agcggaaaaa gctttgggaa
     2761 aaatcgctgg gaagctcatt ccgctatgaa gacgggctga cggaaagact cgcgaaagat
     2821 tttcagctca caggcggaag catttacaat atcgtttccg acggcgtgat caaggcgctg
     2881 aaagaaaaaa caggtgtgat cacgtatgag ctgctggcga atgcgctgcg ttcggaattc
     2941 accaaaacag gaaggaagtt cgaaatatgc accgacgaga tggtctacca gaacccggtg
     3001 cggagattcg gccctggtta tgaaagcagg acaatgatct gaaattcaca gccagcaatt
     3061 cattaaccgc ggaaatgatc gtatgaaaac ctacgccgac cgccacgaag aagaaacccg
     3121 gacgctgaaa cgtaaaaaag atatttacgg aacagcaggt cttcagcgca tgattgattc
     3181 actgggaaca gagggcatca tgcggatggt ggaccacgct tcc
//
LOCUS       DASTSF010000162         7619 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1169445,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000162 DASTSF010000000
VERSION     DASTSF010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 7619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7619
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1169445"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..304)
                     /locus_tag="VFU15_04720"
     CDS             complement(<1..304)
                     /locus_tag="VFU15_04720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717109.1"
                     /translation="MNILRDDNLDTEYDYLLLDASGAVIARITFLPGGTVNAFPVDFG
                     FFSSWNRKGQVLSILDQEGRLIAYFNAEGLDDRGRKLIWGSIKMGGFWLEYSLREAV"
     gene            complement(312..1787)
                     /locus_tag="VFU15_04725"
     CDS             complement(312..1787)
                     /locus_tag="VFU15_04725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717110.1"
                     /translation="MNVSSGESNGEYHFPSSYYVPASYWQKSDSMHPELLHAKIGCYQ
                     RLIIHYGLNLYCAACRQLALLTTEHPEAWEAIDAHTRLLLNGTIGETGCIRAWSDEGG
                     MWQYGDNGTTFNRADNGAYFFGVISDRFSLADPFTAQTHFPAGQGGSRLEWIFYDPFL
                     GENTWAALIAPMQVLFRRRSVEPTALSGNPAELQLAMSLIPACRAMQSRVGGVYARPA
                     PHGKPLERLISNETNLTLYAGLCMMCQLFPEISADPAVKELMEGLLSYFRTVLFGREE
                     DGRLRMHTCGSFTGDEFHPGIAADGEPARFAVDVHTWGMSILGVDEIDTMHGTGTCFE
                     LWQSVKQHAGFYPSQQPGEPIRGVGYSSGALGDPVHDICSPEWTFGAINMCRILAAEY
                     DAPGQHYAPHLAKQLREDEKNMLDGVASFESSSELERNARSYPYVNKRCETGFGWHAF
                     PVESLCATSWAILVRNGFNPFRTGGACLSTLNNRISSTTKA"
     gene            complement(2248..3027)
                     /locus_tag="VFU15_04730"
     CDS             complement(2248..3027)
                     /locus_tag="VFU15_04730"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HEU4717111.1"
                     /translation="MKTISVIIAAYRAADFIRSAIDSVLAQQLPDNYRLQLILGIDGC
                     ESTKQAVSTIRDERLLVLNMDHNYGTYITFNTMMRFAAGDLIARFDADDIMLEGYLRR
                     QIEILEQHPDVYLTWTRSQYIDPEGRHITDESQDIITAPAYWEIRSASDGQFVMRKEL
                     WSSLGGFKPWRCNSDTDFLFRMQFMGFKEYGILEVLYQRRIHPGSLTQCRETGYDSEM
                     RRAVREIMNRERNARTTKEECRVEPVVGRLADAEGCTLPEL"
     gene            complement(3024..3914)
                     /locus_tag="VFU15_04735"
     CDS             complement(3024..3914)
                     /locus_tag="VFU15_04735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717112.1"
                     /translation="MNNPTVCIVDKDLPLNKDFYESLMARTSAEGIRVSYDNGLGEAS
                     EKAAAVLLATPVEVNDIDDSRIVLGLRTINRRERMEIAAQVVPEKLIARWASPGDGQE
                     LTEVLRGWGRSEYVFKYDWSAGRKGVKLLRDGPDQLPEDYSPHRDIVMEYLDDDPYTY
                     KADLCCGVLLNAWFLRTVSIAADNFNAYTVNPAQYVLPEDVRQQLEKLSAELMRYGSG
                     YISIDMMKYKGEFRIIEINTNSVGRNISWEHFGDTYLDTYPQGLKRLLAGLSVLPTLG
                     DLRYWETRPGKILPPVNTAG"
     gene            complement(3907..4746)
                     /locus_tag="VFU15_04740"
     CDS             complement(3907..4746)
                     /locus_tag="VFU15_04740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717113.1"
                     /translation="MYTVRVFQQYTGADYFRIVAHEEINAQQFCPGDLVIFCDIDRCN
                     DEELEELKTAYRNILGTGCSILNNPAKVLRRYQLLQGLHADLTNDFRVYRPGELPATE
                     KIRFPVFVRDELEHNGPGSELIHTRVELESILANSPPPNPLVCEFIDTTIKGHYHKYG
                     AFVLDGKIIPRHFFLSGSWNVKSASGDTGHSQQLEIDYVLKNPHAEELQQIAEYAHIE
                     FGRIDYAITEKGIQVFEINTNPTVIDEGDIAAGNPRHFITNWFIDAFSKELKNLKLSA
                     HYE"
     gene            complement(5015..6058)
                     /locus_tag="VFU15_04745"
     CDS             complement(5015..6058)
                     /locus_tag="VFU15_04745"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HEU4717114.1"
                     /translation="MKKTRNFLTAVAAALLPLLSAGQDIHFSQLTETPLLLNPAQAGL
                     SHDVLAEINYKDQWRSVTANPFRTFNVSGDFAFMKKQNGNHLGAGIDFFSDKAGDGVM
                     GTTTGQLHFAGVLAVNDKNLISAGIYGGFGQRSLSYQNLYWDNQYIGGSLDLTAPSME
                     PTAIPNRTYADMGAGLAWFYGKGHSTMTSNDGATFTAGISVWHVNKPVYTFYGNNDMR
                     LPMKVVAHGNADIGIKNYSLVLEPGYIVMVQGGHHEINVGMLVKYLMQEASHYTGRKK
                     PASFVLGGYYRVGDAANIITAYEFNSFRIGFSYDINLSDLTAASQSRGGFEISLRYMM
                     IDDGNNKNSKFFN"
     gene            complement(6063..>7619)
                     /locus_tag="VFU15_04750"
     CDS             complement(6063..>7619)
                     /locus_tag="VFU15_04750"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HEU4717115.1"
                     /translation="STVPDNSLGSLIRLTIGAPHSNAETITSYDFNTFAPRDTFCVGD
                     IVALFFSPYNYSDHSTYMWQCNGINGGNPFVSPSGANSNSLYVNLNGAGVLIFRVQET
                     NYGCTGSWSPFDTIVVLGPPNVTITGPHFVCQGDTDTYTTLFNNNTYYSWGSTGGNIV
                     DTSNNVIDMSFPNTGNFQITLNAINQCASLSSVYNVTVNPYPAANAGNDTVICSNTPV
                     PLSTPTGSGYVYSWSDGTSTISTSNAVVVSPTVTTTYVVSVSIVGGCTSHDTVTVIVN
                     SIDDTTSTTQAGCNVNDATATVTPTSGVPPYTYLWSNGQTTQTAVALAPGTYTCTVTD
                     ALGCSYSSTVVITSISSLNPDAGPTATITAGQSTQLNGSGGVNYSWSPSTGLSCTNCQ
                     NPLASPSVTTTYTLTVTDSTGCPATDTVTVFVDIDCGTVFVPNAFSPNGDNQNDVLFV
                     RGACIQYMDFSVYNRWGERVFHSTDPAVGWDGTWRGVPCEDAVFMYVLKGTLLDGTQI
                     DQKGNVSLIK"
BASE COUNT         1762 a         2063 c         1968 g         1826 t
ORIGIN      
        1 agacagcttc gcgaagacta tattccagcc aaaagccgcc cattttaata ctgccccaga
       61 taagcttgcg gccacggtca tcgagtcctt ctgcattaaa ataagcgatc agcctgcctt
      121 cctggtcaag aatggaaagc acctggcctt tgcggttcca cgaactgaaa aacccgaaat
      181 caactggaaa tgcatttacg gtcccgccgg gaaggaacgt gatccgggct atcacagcgc
      241 cgcttgcatc cagcagcaaa tagtcatatt cagtgtccag gttgtcatct cgtaatatgt
      301 tcattttttc actatgcttt tgttgttgat gaaatgcggt tgttcaatgt gctcaggcag
      361 gctccgccgg tgcggaaagg gttgaagcca ttccgtacga gaatcgccca cgaggttgcg
      421 cataagcttt caacaggaaa ggcatgccac ccgaatcctg tttcgcaacg tttattcaca
      481 taaggatagg aacgggcgtt acgctccagc tccgatgaag actcgaacga tgcgacaccg
      541 tccagcatgt tcttttcatc ttcacgcaat tgttttgcaa gatgcggagc gtaatgctga
      601 cccggcgcat cgtattcggc ggcaagaatc cggcacatgt tgatcgctcc gaaagtccat
      661 tccggcgaac agatatcatg caccgggtcg ccgagcgcgc cggatgaata gccgactccc
      721 cggatgggtt caccgggttg ctgcgacgga taaaagcctg cgtgctgctt cacactctgc
      781 cagagttcga aacacgttcc cgtaccatgc atcgtatcga tctcatcaac gccgagtatg
      841 ctcatccccc acgtatgaac gtcaactgca aaccgggcag gttcgccatc cgccgcgatg
      901 ccgggatgaa attcatcgcc ggtgaatgat ccgcaggtgt gcatgcgcag cctgccatcc
      961 tcctcccgcc cgaagagaac tgtgcggaaa taggaaagca gtccttccat tagttcttta
     1021 acagccggat cggcactgat ctcagggaat aattgacaca tcatgcacaa gcctgcataa
     1081 agcgtcaggt tcgtttcatt cgagatgagg cgttccagcg gtttgccgtg cggcgccggt
     1141 cgcgcataga cgccgccaac ccggctttgc atggccctgc aggcaggaat aagcgacatc
     1201 gccagctgca attcagccgg gttcccgctg agcgctgttg gttcaacgga tcgcctgcgg
     1261 aaaagaacct gcatgggtgc aatgagcgcc gcccaggtat tctctcccaa aaacggatca
     1321 taaaagatcc actcgagccg ggaacctccc tgtccggccg ggaaatgtgt ttgcgcggtg
     1381 aacggatcgg ccagggaaaa acgatcactg atcacaccaa aaaaatacgc gccattatca
     1441 gcacggttga acgtggttcc gttatctccg tattgccaca ttccgccttc atccgaccag
     1501 gcacggatgc aaccggtttc accaatcgtt ccgttcagca aaagacgggt atgcgcgtcg
     1561 atggcttccc atgcttccgg atgttctgtc gtcaggagag cgagctgcct gcaggctgcg
     1621 caataaaggt taaggccgta atgtataatc agccgctggt agcagccaat ctttgcgtga
     1681 agcaattccg ggtgcatcga atcgcttttc tgccaatagg aagccggtac ataataactg
     1741 gaaggaaaat gatattcgcc gttgctttcg ccggacgata cgttcaaaac gaacgttacc
     1801 aggtgttccg tactgttttc tttgtgcgaa aagttgcgcg tgggagttat ccggctgccg
     1861 attatatttc gtaaatcaaa ccttgggtta ctcatgtcag aattgacaag atagcaatgt
     1921 aaagttgttc ttcaatatcc tgcatcaacg gattcataaa aaaaattggg cgtctcctgc
     1981 acagcccgtg atgtattcat ttacataatc aattctgccg gacatttaag aaatgccctg
     2041 ttgatgccta ttgaaaagat tattgaaaat cgtacgccgt ctcaaaaatg atcatgggta
     2101 aaaagtatcc ggatccgaaa aaccaatgaa gaaaatccat gactggtatt tccgggatgc
     2161 gctgcaacct gtgcgcagct gcgcgcgttt gcggaattcc attgggaaac ctgcacgccg
     2221 gaaccgggat tcacaccggg atttttttta cagctccggg agtgtacaac cctccgcgtc
     2281 tgcaagtctt cccaccaccg gctctacgcg gcattcctct ttcgtggtgc gtgcgttcct
     2341 ttcccggttc attatttcgc gaacagctct gcgcatctcc gaatcatatc ctgtttccct
     2401 gcattgggta agcgaaccgg gatgtatcct gcgctggtaa agcacttcca gtatcccgta
     2461 ctctttgaat cccatgaatt gcatgcggaa caggaaatcg gtgtcgctgt tgcagcgcca
     2521 gggtttaaag ccgccgagcg atgaccagag ctctttgcgc atcacaaact ggccgtcgga
     2581 agcactgcgg atctcccagt aggcaggcgc agtaattatg tcctgtgatt cgtccgtgat
     2641 gtgccggcct tccgggtcaa tgtattgact gcgcgtccag gtcaggtaca catccgggtg
     2701 ctgctcgagg atctcgatct gccggcgcag gtaaccttcg agcatgatgt catccgcatc
     2761 aaaccttgcg atcaggtcac ccgccgcaaa acgcatcatg gtattgaacg tgatgtaggt
     2821 gccgtaattg tgatccatgt tcagcaccag caatcgctca tccctgatcg ttgaaaccgc
     2881 ctgcttcgtg gattcgcagc catctatgcc gaggatgagc tgcagcctgt agttatccgg
     2941 cagctgctgg gccagcactg aatcgatggc tgagcggatg aagtccgccg cgcggtaggc
     3001 agcaatgatg actgaaatgg ttttcaaccg gctgtgttta caggtggcag aattttaccg
     3061 ggccgggttt cccaatacct gaggtcgccc agcgtgggca gcacggacag gccggccagc
     3121 aaacgtttga gaccctgcgg atacgtatcc aggtatgtat cgccgaaatg ttcccatgaa
     3181 atgttccgcc ccacgctgtt cgtgttgatc tcgatgatcc tgaactcacc tttgtacttc
     3241 atcatgtcga tgctgatgta gccgctgccg taacgcatca gttcagcgct cagcttttca
     3301 agctgctgcc gcacatcttc cgggagcaca tactgcgcag gatttactgt gtacgcatta
     3361 aaattatccg cggcgatgga gaccgttcgc aaaaaccagg cgttcagcag cacgccgcag
     3421 cacaggtctg ctttgtaggt gtacggatca tcatcgaggt attccatcac aatgtccctg
     3481 tgcggtgaat aatcttccgg cagctgatcg ggaccatcgc gaagcagctt cactcctttt
     3541 cgcccggccg accagtcgta tttgaaaacg tattcgctcc tgccccaccc gcgcagcact
     3601 tcggtgagct cctgcccgtc cccgggtgat gcccagcgtg caatgagttt ttccggcacc
     3661 acctgcgcgg caatttccat ccgttccctg cggttgatgg tgcgaagccc cagcacaatc
     3721 cggctgtcgt caatgtcatt cacctcgaca ggtgtggcga gcagaactgc cgctgctttc
     3781 tccgaagcct cgccaagccc gttatcatag ctgacccgga ttccttcggc agacgttctc
     3841 gccatcaggg attcgtaaaa gtccttattc agcggaaggt ctttatcgac aatgcatacc
     3901 gtgggattat tcataatgtg cggacaactt caggttcttc aattctttgg aaaacgcatc
     3961 tatgaaccag ttcgtaatga aatgccgcgg attgcctgca gcaatatcac cttcatcaat
     4021 gacagtggga ttggtgttga tctcaaacac ctgtatgcct ttttccgtaa tggcgtaatc
     4081 aatacgtccg aattcaatgt gtgcatattc agcgatctgc tgcagttctt ccgcatgcgg
     4141 atttttcaga acgtaatcga tctccagttg ctgcgaatga ccggtatcgc cggaagcgct
     4201 tttgacattc catgaaccgg agagaaaaaa atgccgggga atgatctttc cgtccaggac
     4261 aaaagctccg tacttgtgat aatgtccttt gatcgtcgta tcgatgaatt cacacaccag
     4321 cggattaggc ggcgggctgt tcgcgaggat gctttccagc tccacgcggg tatgaataag
     4381 ttccgagccc ggaccattgt gctcgagctc atcccgcacg aacacaggaa aacggatctt
     4441 ctccgtggcc ggcagctcac cgggccggta cacccggaaa tcattggtga gatcggcgtg
     4501 caatccctgc aacagctggt agcgcctcag cacctttgcc gggttgttaa gtatgctgca
     4561 gccggttccg aggatgttac gataagcagt cttcagctcc tcaagctcct catcgttgca
     4621 cctgtctata tcgcaaaaga tcacgagatc tccgggacag aactgctgcg cgtttatttc
     4681 ttcgtgcgca acgatccgga agtaatctgc acctgtgtat tgctgaaata cacgaaccgt
     4741 atacatatgt tcaccacgga tcaggaaata caacatccgg cgtttttttt gtcatcgcca
     4801 aaataatgat tccggtccag tgtgcaaatt cccgctattt atttactcta ttgtaagctc
     4861 gcctgaaaca ctgaaggcaa ctaaacaacc acccgcaaag caggtggatt tccggggtaa
     4921 attaaaatca cgaaagtttg ttgctgctac ctggaaacct ggcggatttc acgactctcc
     4981 ccgcactttt ccatcgttaa cgcgatactt atcttcaatt aaaaaatttc gaattcttat
     5041 tgttgccgtc gtcgatcatc atgtaacgca gcgaaatctc gaagccgcca cgcgactggc
     5101 tggctgcagt caggtcggac aggttgatgt catagctgaa cccgatgcgg aaggaattga
     5161 attcatacgc agtgatgatg ttcgcggcat cgcccacacg gtaatacccg ccgagaacga
     5221 acgacgccgg ttttttccta cccgtgtaat gcgacgcttc ctgcatgagg tatttcacaa
     5281 gcattcccac gttgatctcg tgatgaccgc cctggaccat gacgatgtaa ccgggctcga
     5341 gaacaaggct gtaatttttt ataccgatat cggcattccc gtgcgcaacc actttcatcg
     5401 gcaggcgcat atcgttgttg ccgtaaaaag tgtaaacggg tttgttgacg tgccacacgg
     5461 aaattcccgc ggtgaatgtc gcaccgtcat tcgatgtcat ggtgctgtgg ccttttccgt
     5521 agaaccacgc gagccctgcg cccatatccg cgtacgtgcg gttcggaatt gccgttggtt
     5581 ccatggacgg cgccgtaaga tcgagcgacc cgccgatgta ttgattatcc cagtaaaggt
     5641 tctggtagct gaggctgcgc tgaccgaatc cgccgtatat gccggccgag atcaggttct
     5701 tgtcgttcac cgcaagcacg cctgcaaaat gtaattgccc ggtcgttgtg cccatgacgc
     5761 cgtcaccggc cttgtcggaa aaaaaatcga tccctgcacc gaggtgattt ccgttctgct
     5821 ttttcatgaa cgcaaaatcc ccgctgacat tgaatgtgcg gaacgggttg gcggtgacgc
     5881 tccgccactg gtctttataa ttgatctctg cgagcacatc atgcgagagc ccggcctgcg
     5941 ccggattcaa tagcagcggc gtttccgtca gttgcgagaa gtgtatgtcc tggcccgctg
     6001 aaagaagagg cagcagcgca gcggcaacag ccgtcagaaa attcctggtc tttttcattt
     6061 gatcacttta tcaaagaaac gtttcccttt tgatctatct gtgttccgtc gagcagggtt
     6121 cccttcagca cgtacataaa tacagcatct tcacacggaa ccccacgcca tgttccatcc
     6181 cacccgactg ccggatcagt ggaatggaaa acgcgttcgc cccagcggtt gtaaacggag
     6241 aaatccatgt actggatgca ggcaccccgc acaaacagga cgtcgttctg gttatcaccg
     6301 ttcggcgaga aagcgttcgg cacaaagaca gttccgcaat cgatatcaac gaaaacagtt
     6361 acggtatctg tagcgggaca acctgtggaa tccgttacag tcaacgtata cgttgtcgtc
     6421 acgcttggcg atgcaagcgg gttctggcag ttggtgcagc tcagcccggt ggaaggcgac
     6481 cagctgtagt tcaccccgcc cgatccgttc aactgcgtcg actggccggc cgtgatggtg
     6541 gcggtcggtc ctgcatcagg attgagcgag ctgatgcttg tgatgaccac ggtcgaagaa
     6601 tacgaacagc cgagcgcatc ggtgaccgtg catgtgtatg tcccgggggc gagcgcaaca
     6661 gccgtctgcg tggtctgccc gttgctccag agatacgtgt aaggcggaac tccgctggta
     6721 ggcgtaacag ttgcagttgc atcgttgacg ttgcatcccg cctgcgtcgt ggacgtggtg
     6781 tcgtcgatcg agttcacgat gactgtcact gtatcatgac tcgtacaacc gcccacgatg
     6841 gaaacggaaa cgacgtatgt cgtcgtaaca gtgggcgaga cgacaaccgc attgctcgta
     6901 cttatcgtac tcgttccgtc cgaccaggaa taaacataac cggatcccgt tggcgtggag
     6961 agtggcaccg gcgtattgct gcatatcacg gtatcgtttc ctgcattcgc cgccgggtac
     7021 ggatttaccg ttacgttgta aacagatgac agcgacgcgc actggttgat ggcgttcagc
     7081 gtgatctgga aattaccggt attggggaac gacatgtcga tcacgttgtt cgacgtatcc
     7141 acgatgttcc cgccggttga gccccagctg taatacgtgt tgttgttgaa cagcgtggtg
     7201 tacgtatccg tatcgccctg gcacacaaaa tgaggtccgg taatcgtgac gttcggcgga
     7261 ccgagcacaa cgatcgtatc gaacggagac cacgaaccgg tgcacccgta attcgtttcc
     7321 tgcacgcgga aaataagcac gcctgcgccg ttgaggttga cataaagtga attcgaattg
     7381 gctcccgacg gcgaaacaaa tggattgccc ccgtttattc cgttgcattg ccacatgtac
     7441 gtggaatgat ccgaataatt atacggcgag aagaacaacg ccacgatatc gcctacgcag
     7501 aatgtgtcac gcggtgcgaa cgtgttgaaa tcatacgacg tgatcgtttc ggcattcgaa
     7561 tgcggtgcgc cgatcgtcag gcggatgagg cttccgagcg aattatcagg cacggtgga
//
LOCUS       DASTSF010000163         6518 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1678268,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000163 DASTSF010000000
VERSION     DASTSF010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 6518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6518
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1678268"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1795
                     /gene="alaS"
                     /locus_tag="VFU15_04755"
     CDS             <1..1795
                     /gene="alaS"
                     /locus_tag="VFU15_04755"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008513325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEU4717116.1"
                     /translation="VINIAMRVIADHVRAISFAIADGQLPSNTGAGYVIRRILRRAVR
                     YGYQSLNLRDPFICRLVPVLVNEMGEFFPELNTQKELIAKVIREEESAFYRTLETGLK
                     KIDAICIQLANEKKSKTVPGKEAFELYDTFGFPFDLTALIARGYGLEIDEEEFKKELE
                     KQKDRSRAATSLDTDDWVELLKDVTTNFIGYSELESDAKIIRYRKIKSKGKESFQLVL
                     DVTPFYAESGGQVGDTGWLETANEKVRVYDTKKENGVIVHFTDKLPSDPSKMLHAKVD
                     AEKRALTADNHSATHLLHAALRKVLGTHVEQKGSLVNDEYLRFDFSHFQKMTDEEIAE
                     VEHIVNEKIRANISSVIREMAIGDAKKLGAMALFGEKYGDVVRVVTFDENYSIELCGG
                     THVKSTGEIGFFKITGESAIAAGIRRIEAITSVKAEQFLHEQLHTMREIREALKNPKD
                     LVKSVQQLLEEKSALEKQVDALLRQKAKTIKSELIGKAEKVNGIHFIAQKIDLGSAEA
                     IKDLAFELRNQLDPLFLVLGAEVNGKASLTLMISDDLVKEKKLNAGQIIRDLAKEIQG
                     GGGGQPFFATSGGSNVAGIPKALEKAKAVIS"
     gene            complement(1785..2747)
                     /locus_tag="VFU15_04760"
     CDS             complement(1785..2747)
                     /locus_tag="VFU15_04760"
                     /inference="COORDINATES: protein motif:HMM:NF016041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HEU4717117.1"
                     /translation="MALAYDSIANFFSNVQANVGPQVAKLIKHPSWTNPFTALFTVWI
                     VAFILEHTLPKMKNYPVVGRKGFWLDIFYLFFIDILFGIIGFFAVTYTVEYIFIHGMA
                     SVGVKIPVMDLSVLPKVVQFLIFFIVMDFCQFFAHYLLHRVEFFWKFHKIHHAQETLG
                     FASTRHFHFGEYLVLRPLGWIPFGVFGFSDEKYLYFYAIYMWIAYSLVFFSHCNVKIN
                     FGILNRIFITPNTHYWHHAKNIPRRYGVNYASVLTIWDFLFGSFYLPDDPKTQPILGV
                     PNNDVPANFVGQTWYPFKTLFSRKKDRQSSVLDTKKKGKAETKK"
     gene            complement(2752..3552)
                     /gene="mazG"
                     /locus_tag="VFU15_04765"
     CDS             complement(2752..3552)
                     /gene="mazG"
                     /locus_tag="VFU15_04765"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013619644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HEU4717118.1"
                     /translation="MNSLEQKKEAFARMLTIMDELRTQCPWDKKQTMESLRHLTIEEM
                     YELADAILEKNTDGIRKELGDVLLHIIFYARIASETNDFDIADVIHAECDKLVARHPH
                     IYGDVKVENEEQVKENWEKIKLKEKDSITGEGFRSVLAGVPVSLPAVVKAWRIQEKAR
                     AVGFDWEKPEQVWEKVQEELAELNAEVKNGKSQEKTEEEFGDVLFAMINYARFIGVNP
                     EDALERTNKKFIKRFQYLEKESQKDGKKMGQMTLAEMDEYWNRAKEIS"
     gene            3709..4839
                     /locus_tag="VFU15_04770"
     CDS             3709..4839
                     /locus_tag="VFU15_04770"
                     /inference="COORDINATES: protein motif:HMM:NF020965.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HEU4717119.1"
                     /translation="MRKLFSILCFLFLAGALAAQADTVQAVHCERYLLGGNVQYGFLW
                     SHRYNMGHLVKKHLVAGEIDVWHTTNGDQSWHCPFHYPWTGMTLVCIPLGNPDELGTA
                     IGLYPFINFPLGKRDRKFKLNMRYGWGIGWLTNPFDPIENHKNVAIGSHLNTCFVLRL
                     NGMWKMSERSNMEFGLGMTHFSNGAMRLPNLGINLPMLSVGYNYEISRNQCRKRSDAY
                     CNRIACNDSLMADHTWHFSAIIVTGMNDIDPPGGGRFGVLNLLTYAMKQTQRKARFGC
                     GIDVMYSQAIQHKLVYDSVNVNAFGALQPGFKLCYELVLGRLSLPAEMGVYLYSRYKD
                     NGPVYNRWGLRYLLGKHTELDFSLKTHFAKAEYFELGFGWRI"
     gene            4841..5581
                     /locus_tag="VFU15_04775"
     CDS             4841..5581
                     /locus_tag="VFU15_04775"
                     /inference="COORDINATES: protein motif:HMM:NF022441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2807 domain-containing protein"
                     /protein_id="HEU4717120.1"
                     /translation="MKARFILFVLFPFVLLFPQCKKENACDCFKGTGDIVTEERQLQP
                     YHNVYVEDNVNLIFVEDTMNIVRVEAGKHLIKLVKTESDGEWLRIRNDNKCNFMRRYG
                     IPINVYIHYVRNSIYHIKTKGTGEITNANPCTSDSIDLDVESSGDITFEMNSPAVYTH
                     QHGAGDITLTGHSDNVIIYSRGTGFTITDGITSGYTWVSTNTTGKITVAPSNLLIVEI
                     LGPGNVYYKGTPASIVSSELNTGKLLPL"
     gene            5609..>6518
                     /locus_tag="VFU15_04780"
     CDS             5609..>6518
                     /locus_tag="VFU15_04780"
                     /inference="COORDINATES: protein motif:HMM:NF028426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5103 domain-containing protein"
                     /protein_id="HEU4717121.1"
                     /translation="MKRYRILFALLLFSLSAELAKASVPDSAEEYFSDKTLRYEDKIY
                     VPEIKTVVLSPDPSVLAPPVISLNSTDVLYLSFDDLGADFKTYTYTLIHCTAKWEPSN
                     ALVSEYLDGFAQNPITDYQYSRSTLQKYTHYSASFPNENVKLTKSGNYLLKVYTDNDE
                     DSIVFTRRFLVYENNVTVDASVHAATIVSDRNFKQEVDFNIGYNQADIANPYSQIYPV
                     ILQNFRWDNAISGLQPQFVKDGQLVYDYDDVNVFSGGNEFRNFDTRSLRYKSERVEDI
                     EKDSNNLYHVYLLPDDKRAYKRFSTTN"
BASE COUNT         1624 a         1783 c         1671 g         1440 t
ORIGIN      
        1 agtgatcaac atcgccatgc gcgtgatcgc cgaccatgtg cgcgcgatct cgttcgccat
       61 cgccgacggc cagctgcctt ccaataccgg cgcgggctac gtcatacgcc gcattctccg
      121 ccgcgcggtg cgctacggtt accagtcgct caacctgcgc gatccgttca tctgccgcct
      181 cgtcccggtt ctcgtgaacg agatgggcga attttttccc gagctgaaca cgcagaaaga
      241 gctcatcgca aaagtgatcc gcgaagaaga aagcgcattc taccgcacgc tggaaaccgg
      301 gctgaaaaag atcgacgcga tctgcatcca gctggccaat gaaaaaaaat cgaaaaccgt
      361 tccgggaaaa gaagcgttcg agttatacga cacgttcggt tttcctttcg accttactgc
      421 gctcatcgca cgcggctacg gacttgaaat tgacgaagaa gaattcaaaa aagagctgga
      481 aaagcagaaa gaccgttcac gcgccgccac ttcgctcgac acggacgact gggtggaatt
      541 gctgaaagac gtcacgacga atttcatcgg ctattcggaa ctggagtcgg acgcaaaaat
      601 catccgttac cggaaaataa aatcgaaagg aaaagaatcc ttccagcttg tgcttgatgt
      661 caccccgttc tacgcggaaa gcggcgggca ggtgggcgac accggctggc tcgaaacggc
      721 gaatgaaaaa gtgcgcgtct acgacacgaa aaaagagaac ggcgtcatcg tgcatttcac
      781 cgataagctt ccctccgatc cgtcgaaaat gctgcatgca aaagtggatg ctgaaaagcg
      841 tgcgctcacg gccgacaacc acagcgccac acatctgctc catgccgcgc tgcggaaagt
      901 gctgggcacg catgtcgaac agaaaggttc gctggtaaac gatgaatacc tgcgtttcga
      961 cttttcgcat ttccagaaaa tgacggacga agaaattgca gaggtggaac acatcgtgaa
     1021 tgaaaagatc cgcgcgaaca tttcctccgt catccgcgag atggcgatcg gcgacgcgaa
     1081 aaagctcggc gcgatggcgc tcttcggtga aaaatacggt gacgtggtgc gcgtggtgac
     1141 gttcgacgag aattattcca tcgagctttg cggcggaacg cacgtgaaat cgaccgggga
     1201 gataggcttt ttcaaaataa cgggcgaaag cgcgatcgct gccgggatcc ggcgcatcga
     1261 agcgatcact tccgtaaaag cggaacagtt cctccacgag cagctgcaca cgatgcgcga
     1321 gatccgcgag gcgctgaaaa atccgaaaga cctggtgaaa agcgtccagc aattgctgga
     1381 agaaaaatca gcgctcgaaa aacaggtgga tgccttgctt cgccagaaag cgaaaaccat
     1441 caagtcggag ctgatcggga aagcggaaaa agtgaacggc atccatttca tcgcgcagaa
     1501 gatcgatctc ggttccgcgg aagcgataaa agatcttgct ttcgaactgc gcaaccagct
     1561 ggacccgctg ttcctcgtcc tcggcgcaga agtgaacgga aaagcctcgc tcacgctgat
     1621 gatctcggac gatctcgtga aagaaaagaa gctcaacgcg ggacagatca tccgcgacct
     1681 tgcgaaagaa atacagggag gtggaggcgg tcagcctttc ttcgccactt ccggcggaag
     1741 caacgtggcc ggaattccga aagcgctgga aaaagcgaaa gccgttattt cttagtctcc
     1801 gctttacctt tcttctttgt atccagcacg gaagactgcc ggtctttttt ccggctgaac
     1861 agcgttttga acggatacca cgtctgtcct acgaaattgg cgggaacatc attgttcggc
     1921 acgccgagaa tgggctgcgt cttcggatcg tccggaagat agaacgagcc gaaaagaaaa
     1981 tcccagatcg tcagcaccga cgcatagttc acgccgtatc tgcgcgggat gttcttcgcg
     2041 tggtgccagt aatgcgtgtt gggcgtgatg aagatcctgt tgagaattcc gaaattgatc
     2101 ttcacgttgc agtgcgaaaa gaaaacgagc gaatacgcga tccacatgta gatcgcatag
     2161 aagtacaggt atttttcatc cgaaaagccg aatacaccga acgggatcca gcccagcggg
     2221 cgaagcacga ggtattcccc gaaatgaaaa tgacgcgtcg aagcaaagcc cagcgtttcc
     2281 tgcgcgtgat ggatcttgtg gaacttccag aaaaattcca cgcggtgcag gaggtaatgc
     2341 gcgaagaact ggcagaaatc catgacgatg aaaaagatga ggaactgcac cactttcgga
     2401 agaacggaaa gatccatcac cgggatcttc acgccgaccg acgccatgcc gtgaatgaaa
     2461 atgtattcga cggtgtatgt gacggcgaaa aacccgatga tgccgaaaag aatatcgatg
     2521 aaaaaaaggt agaagatatc gagccagaaa cctttccgcc cgacaaccgg gtagttcttc
     2581 attttcggca gcgtatgctc gagaataaat gcaacgatcc acacggtgaa caacgcagtg
     2641 aacgggttgg tccacgaagg atgcttgatc agcttcgcga cctgcgggcc cacattcgcc
     2701 tgtacgttgg aaaagaaatt cgctatggaa tcgtatgcga gcgccatgcc ttcagctgat
     2761 ctctttcgcg cggttccagt attcgtccat ttcggccagc gtcatctgcc ccattttctt
     2821 gccgtctttc tgcgattcct tttcgaggta ctggaaacgt ttgatgaatt ttttgttcgt
     2881 tcgttcgagg gcgtcttcgg ggttcacgcc gatgaagcgt gcgtaattga tcatggcgaa
     2941 gagcacgtca ccgaactctt cttccgtttt ttcctgtgat ttcccgtttt tcacttctgc
     3001 gttcagctct gcaagttctt cctgcacttt ttcccacacc tgttccggtt tttcccagtc
     3061 gaaaccgacg gcgcgcgctt tttcctggat ccgccacgct ttcaccaccg cgggcaggga
     3121 aacgggaaca ccggccagca cggaacggaa accttcgccc gtgatgctgt ctttttcttt
     3181 cagctttatt ttttcccagt tctccttcac ctgctcttcg ttctccactt tcacgtctcc
     3241 gtagatgtgc gggtgacgcg ccacgagctt atcgcattcg gcatgaatga cgtcggcgat
     3301 gtcgaaatcg ttcgtttcac ttgcaatacg cgcatagaaa ataatgtgca gcagcacgtc
     3361 gccgagctcc ttgcggatgc cgtccgtatt cttttcgaga atggcatcgg ccagctcgta
     3421 catttcttcg atggtgagat gacgcaggct ttccatcgtc tgctttttgt cccacgggca
     3481 ctgcgtgcgc agctcgtcca tgatcgtgag catccgcgca aaagcttctt ttttctgttc
     3541 aagagaattc ataaggtgaa attaatacct caacggctcg atctgacggt aacgtggtta
     3601 aaaatcgcga agcgcatgct tcttttccgc tcgaatgtta attcaatacg gctccggggc
     3661 gcgaacgaac attggactca tttattattt ttacacgccc cgttcctgat gcggaaactg
     3721 ttttccatcc tgtgctttct ctttttggcc ggcgctctcg cagcacaggc agataccgtg
     3781 caggcggttc actgcgaacg ctaccttctc ggcgggaacg tccagtacgg tttcctgtgg
     3841 agccaccgct acaacatggg ccacctcgtg aaaaagcacc tcgtcgcggg cgagattgat
     3901 gtctggcaca cgacgaacgg cgaccagtcg tggcattgcc ccttccatta cccgtggacc
     3961 ggcatgacgc tcgtgtgcat tccgctcgga aacccggatg agctcggaac ggccataggt
     4021 ctttacccgt tcatcaattt cccattggga aaacgcgacc ggaagttcaa gctgaacatg
     4081 cgttacggct gggggatcgg ctggctcacg aacccgttcg acccgatcga aaatcacaag
     4141 aacgtcgcca tcggctcgca cctcaacacc tgcttcgtgc tgcgtctgaa cggcatgtgg
     4201 aaaatgtcgg agcgcagcaa catggagttc ggcctcggca tgacgcattt ttccaacggt
     4261 gcgatgcgcc tgcccaacct cgggatcaat ctcccgatgc tttccgtcgg ttacaactat
     4321 gaaatttcgc ggaaccagtg caggaaacgt tcggacgcat actgcaaccg catcgcctgc
     4381 aacgattcgc tcatggccga tcacacgtgg catttcagcg cgatcatcgt caccgggatg
     4441 aacgacatcg acccgccggg cggaggacgt ttcggcgtgc tcaatctcct cacctacgcc
     4501 atgaagcaga cgcagcgcaa ggcgcgcttc gggtgcggca tcgacgtgat gtacagccag
     4561 gccatccagc acaagctggt ttacgattcc gtgaacgtga atgcattcgg cgcgctgcag
     4621 ccgggattca agttatgcta tgagctcgtt ctcggccgcc tttcgcttcc ggcggagatg
     4681 ggcgtttatc tttacagccg ctacaaagac aacggcccgg tatacaaccg ctgggggctc
     4741 cgctacctgc tcggcaagca cacagaactt gacttttcac tgaaaacgca ttttgccaaa
     4801 gccgaatatt tcgagcttgg tttcggatgg agaatatgat atgaaagcac gattcatcct
     4861 gttcgtcctg ttcccgtttg tgctgctttt tccgcagtgc aaaaaggaaa acgcatgcga
     4921 ctgtttcaag ggaaccgggg atattgtgac ggaagagagg caacttcagc cttatcacaa
     4981 tgtctatgtg gaagacaatg tcaatctcat tttcgtggaa gacaccatga atatcgtccg
     5041 cgtggaagcc ggcaagcacc tgatcaaact cgtgaagaca gaatcggacg gggaatggct
     5101 gcgcatccgc aacgacaaca aatgcaactt catgcggcgc tacggtatcc cgatcaacgt
     5161 gtacattcat tacgtgcgca acagcattta ccacatcaaa acgaaaggaa ccggggagat
     5221 cacgaatgcc aatccctgca cgagcgattc catcgacctg gatgtggaaa gcagcgggga
     5281 cattacgttc gaaatgaaca gtccggcagt ttacacacac cagcacggcg cgggcgatat
     5341 cacgcttacc ggtcacagcg acaacgtgat catttattcg cgcggaacag gattcaccat
     5401 caccgacggc atcacgagcg gttacacgtg ggtgagcacg aacacaaccg ggaagatcac
     5461 cgtggcccct tccaacctgc ttatcgtcga gatcctcggt cccgggaacg tttattacaa
     5521 aggaactccg gcgagcattg tctcctcgga actgaacacg ggcaagctgc tcccgctgta
     5581 aggcataatt attgatcaca ataacgcaat gaaacgatac cggatccttt tcgccctcct
     5641 tcttttttcc ttatccgcag agctggcgaa agcgtctgtt cccgattcgg ccgaagaata
     5701 tttttccgac aagacattgc gttatgaaga caagatctac gtgccggaaa taaaaaccgt
     5761 tgtgctcagt cccgatcctt ccgtgctcgc accgccggtc atcagcctga acagcaccga
     5821 tgtgctttac ctcagcttcg acgatctcgg cgccgatttc aaaacgtata cgtacacgct
     5881 cattcactgc accgcgaaat gggaacccag caatgcgctc gtttcggaat acctcgacgg
     5941 ctttgcgcag aatcccatca cggattacca gtattcacgt tcgacgctgc agaaatacac
     6001 acactattcc gcttcttttc ccaatgagaa tgtgaagctg accaaatctg gaaattacct
     6061 tctcaaagtg tataccgaca atgacgaaga cagcatcgtg ttcacacgcc gctttctcgt
     6121 gtatgagaac aacgtaaccg tcgatgcatc cgtgcatgcg gcgacgatcg tttcagaccg
     6181 caacttcaag caggaagtgg atttcaacat cggctacaat caggccgaca tcgccaaccc
     6241 gtattcccag atctatcccg tcatcctcca gaatttcagg tgggacaatg ccatctccgg
     6301 gttgcagccg cagttcgtca aggacgggca gctcgtttat gattatgatg acgtcaacgt
     6361 tttcagcggc gggaacgagt tccggaattt cgacacgcgc agcctgcgct acaaatcgga
     6421 acgcgtagaa gacattgaaa aagacagcaa caatctttac cacgtctatc ttcttccgga
     6481 tgataagcgc gcttacaaac ggttttccac gacgaacg
//
LOCUS       DASTSF010000164         3501 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1951902,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000164 DASTSF010000000
VERSION     DASTSF010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3501
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1951902"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1834)
                     /locus_tag="VFU15_04785"
     CDS             complement(<1..1834)
                     /locus_tag="VFU15_04785"
                     /inference="COORDINATES: protein motif:HMM:NF020678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-N-glycosidase F-related protein"
                     /protein_id="HEU4717122.1"
                     /translation="MKKIFLLFSFALSLPAFLSANPGDTLHVITHNNETVVTNPNTGW
                     NQYANWGVFPSAAVNYRKVLVKMSYKCPSGYACGEWDYIDQIMLRRKGGVDSASLDLE
                     MVRFITPYGNTFNSTWQFAWHADITDFAALLHDSIEIGYVHTGYETNVGKGWAVTLDF
                     ELIEGTPVRDYIGYSTLWNTNHVYGDANNPIENALGADTVVSSPNTYLTRVRINHTGH
                     GADAAYCSEFCDKYRDFLFDGNLVERWHRWRKCGFNALYPQGGTWVYDRGNWCPGSVI
                     YPNIYQFNVTPNSTHTYDVNMEPYTTGSPSANEYIVAYLFQYGQPVNANDAGMDEVLR
                     PSTMYEYNRINPMCANPQVIIRNNGSNNLTSAVIRYGFAGGPYYAYNWTGNLATDAVD
                     TVDLPATLYAPANGAFFKAYFEQVNGGTDAYNYDDTIYSQGNVSQGIWDTDLIFEFKT
                     NNEPQENSYELLDASGNVVYSRAMGSLAANTIYKDTFHLNPGCYRLLFHDIDAEGGDG
                     LSFWANTAGGSGYARMKRINNIIFKSFLADFGSLIDYSFTVGVPNSIPDPAVSNVMLE
                     AWPNPSNGILNLDIGFTKKSEDVTVNVFDSQGKLVKTVFIPADSD"
     gene            complement(1890..2573)
                     /gene="cmk"
                     /locus_tag="VFU15_04790"
     CDS             complement(1890..2573)
                     /gene="cmk"
                     /locus_tag="VFU15_04790"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007805968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEU4717123.1"
                     /translation="MKKITIAIDGYSSCGKSTIAKAVAAKLGYSYVDTGAMYRAVTLY
                     CLEHGIIKDGAFDETEVVRSLENIHLGFHFNPSSGASETLLNGKNVEKEIRDLSVSNN
                     VSPVSKIHDVRVRLVNLQREMGKNKGVVMDGRDVGTNVFPDAELKIFMTADPEVRAKR
                     RYEELLSKGMRVTMEDIRDNLVSRDYEDTHRKENPLTKAPDAVVLDNSDLTKEEQLDF
                     VLGLLKKTI"
     gene            2681..3148
                     /locus_tag="VFU15_04795"
     CDS             2681..3148
                     /locus_tag="VFU15_04795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717124.1"
                     /translation="MSFAPVSLEQLTDEEKEKIVGFGKKEVFRGLWLYFIPALIIGYA
                     VVYINGHYMGLGLEDPTERSVINLALVILAVVPIRLFINSIVRLKNAHNAWQKKIIRG
                     KITSREGKFIVISGQKIIPGSFAGQFSGEGFYIVTMSTVGNYILSVEKKEEAP"
     gene            complement(3168..>3501)
                     /locus_tag="VFU15_04800"
     CDS             complement(3168..>3501)
                     /locus_tag="VFU15_04800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717125.1"
                     /translation="RLVSQREFTGAIGAAAGMLFFLFWYTAIGILSWHFLPGHWMMPV
                     SVVICPFAGLFAWIYHRTWHYIRRRWKTISMFYRNSEMISSLILKRKELIREFEAAAE
                     EWRSARKG"
BASE COUNT          800 a          952 c          912 g          837 t
ORIGIN      
        1 cgtcggaatc cgccggaatg aaaacggtct ttaccagctt gccctggctg tcgaacacat
       61 tcaccgtaac gtcttccgat tttttcgtga agccgatgtc gagattcaaa ataccgttcg
      121 atgggttcgg ccaggcttcg agcatgacgt tggaaacggc gggatcggga atggaattcg
      181 gcacgccgac cgtgaagctg taatcgatca gcgaaccgaa atcggcgagg aagcttttga
      241 aaataatgtt gttgatgcgc ttcatccgcg cataaccgga accgcccgcc gtattcgccc
      301 agaaggaaag tccgtcgccg ccttccgcat cgatatcgtg aaaaagcaaa cggtaacagc
      361 ccggattaag atggaacgtg tctttgtaaa tggtattcgc ggcaagactt cccatcgcgc
      421 ggctgtaaac gacatttccc gaagcgtcga gcagctcgta ggaattttcc tgcggttcat
      481 tattcgtttt gaattcgaag atgagatccg tgtcccagat tccctgcgaa acatttccct
      541 gcgaatagat cgtgtcgtcg taattgtacg catccgttcc gccgttcacc tgttcgaaat
      601 acgctttgaa aaaagcgccg ttcgcaggtg cgtaaagtgt cgcaggaaga tcgaccgtat
      661 ccaccgcatc ggtggcgaga ttcccggtcc agttgtatgc ataataaggt ccgccggcaa
      721 aaccataacg gatcaccgcg gaagtgagat tgttgcttcc gttgttgcgg atgatgacct
      781 gcggattcgc gcacatcggg ttgatacggt tgtattcata catcgtgctc gggcgcaaaa
      841 cctcgtccat tcccgcgtcg ttcgcattca cgggctggcc gtactggaaa agataggcga
      901 cgatgtattc attcgccgac ggacttcccg tggtatacgg ttccatgttg acgtcgtagg
      961 tgtgcgtgga attcggtgtg acattgaact ggtaaatgtt cgggtaaatg acagaacccg
     1021 ggcaccagtt gccgcggtcg tacacccacg ttccgccctg cgggtacagc gcgttgaacc
     1081 cgcattttct ccaccgatgc cagcgctcca cgagattccc gtcgaacagg aaatcgcggt
     1141 acttgtcgca gaactcggaa cagtaggcgg catcggcgcc atgaccggtg tggttgatgc
     1201 gcacgcgcgt gaggtatgtg ttcggagaag aaacaaccgt atccgcaccg agcgcatttt
     1261 cgatcgggtt gttcgcgtct ccgtaaacgt ggttcgtatt ccacagcgtc gaatagccga
     1321 tgtaatcccg cacgggcgtt ccttcgatca gctcgaaatc gagcgtgacg gcccagcctt
     1381 tcccgacatt cgtttcatag cccgtgtgca cgtacccgat ctcgatcgaa tcatgcagca
     1441 gcgcggcgaa atccgtaatg tccgcatgcc aggcaaactg ccaggtggaa ttgaacgtat
     1501 ttccgtaagg tgtaatgaag cgcaccattt caagatcgag cgaagcagaa tccactccgc
     1561 cttttcttcg cagcatgatc tggtcgatgt aatcccattc gccgcaggca taacccgaag
     1621 gacatttgta cgacattttc accagcactt tgcggtagtt cactgccgcc gaaggaaaaa
     1681 cgccccagtt cgcatactgg ttccagcccg tgttcgggtt ggtgacgacg gtctcgttgt
     1741 tgtgcgtgat gacgtgaagg gtatcgcccg ggtttgcgga aagaaacgca ggaagcgaaa
     1801 gggcgaatga aaagagcagg aagatttttt tcatgacatg aaatgctgtg aaaaacaaat
     1861 gtacgcaatt cacgctgcgg gaagcctgtc taaatcgttt ttttcagcag ccccagcacg
     1921 aagtcaagct gctcttcctt cgtcaggtcg gaattgtcga gcacgacggc atccggcgct
     1981 ttggtgagcg ggttctcctt gcggtgcgtg tcttcataat cacgtgaaac gaggttgtcg
     2041 cgtatgtcct ccatcgtgac cctcatgcct ttcgagagca gctcttcata ccggcgtttc
     2101 gcgcgcactt ccgggtctgc cgtcatgaaa attttcagct ctgcatccgg gaaaacattc
     2161 gtgccgacat cgcggccgtc catcaccacg cctttgttct tgcccatttc gcgctggagg
     2221 ttgacgagac gaacgcgcac gtcatggatc ttgctcacgg ggctgacgtt gttgctcacg
     2281 ctgagatcac ggatctcttt ctcgacgttc ttcccgttca gcagcgtttc cgatgcgccg
     2341 gaagaaggat tgaaatggaa accgaggtga atattttcca gcgaacgcac cacttccgtt
     2401 tcatcgaacg cgccgtcctt gatgatcccg tgctcgaggc aatacagcgt caccgcgcgg
     2461 tacattgcac ccgtgtccac gtagctgtag ccgagctttg ccgcaactgc tttcgcgatc
     2521 gtacttttcc cgcaggagga atacccgtca atagcgatcg tgatcttttt catggatgag
     2581 cgtccgtgcc ggctccttaa cggtgatgcc ggttttattt ttacgaaaat aaacaaccct
     2641 tcgtggcccg cgcatttgta tttttgaaaa aatttcagct atgagctttg ctcccgtttc
     2701 actggaacaa ctgacggacg aggaaaagga gaaaatcgtc ggtttcggaa aaaaagaagt
     2761 tttccgcgga ctctggctgt atttcatccc ggcccttatc atcggctacg cggtcgttta
     2821 catcaacggt cattacatgg gactcgggct ggaagatccg acggaacgtt cggtcatcaa
     2881 tctcgctctt gtcattttag cggttgttcc catccgcctt ttcatcaaca gcatcgtgcg
     2941 gctgaaaaac gcccacaatg cctggcagaa aaagatcatc cgcggaaaga tcacctcgcg
     3001 ggaaggaaag ttcatcgtta tttccgggca gaaaataatt cccggctcat tcgccggtca
     3061 attcagcggc gaaggatttt acatcgttac catgagcacg gtcggaaatt acattctctc
     3121 cgtcgagaaa aaagaggaag ctccctgaag atccgacgat ctgcaattca tcctttcctc
     3181 gcgcttctcc actcttccgc ggccgcctcg aattccctga tcagctcttt ccgcttcagg
     3241 atcagcgacg agatcatttc actgttgcga tagaacatcg agatcgtttt ccatctcctg
     3301 cggatgtaat gccacgtccg gtggtagatc cacgcgaaaa gtccggcaaa cgggcagatc
     3361 accaccgaca ccggcatcat ccagtgtccc ggtaaaaaat gccaggacag gatcccgatc
     3421 gcggtgtacc agaaaagaaa gaagagcatg cccgccgctg caccgatcgc accggtgaat
     3481 tcccgctgcg aaaccagcct g
//
LOCUS       DASTSF010000165         7729 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1248281,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000165 DASTSF010000000
VERSION     DASTSF010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 7729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7729
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1248281"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2031
                     /locus_tag="VFU15_04805"
     CDS             <1..2031
                     /locus_tag="VFU15_04805"
                     /inference="COORDINATES: protein motif:HMM:NF012646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CUB domain-containing protein"
                     /protein_id="HEU4717126.1"
                     /translation="DNNIDPLRTGWWPWLHTTYYNQSGIPGACTKVKGHMHFPNQTHN
                     DPGPNWDWDYYYRLINNNPPAATQLTAASGNFYDSGGASANYSDDERSTWTIAPPNAT
                     SVTISFTSFDVENTWDYLFIYDGTDINAPLIGYYTGTSNPGTITSSGGALTFEFRSDC
                     STNATGWAATWSSTINNTSTDIIPPTTALSINGTWQTTDFPATFSDSDNVGGSGLEKS
                     FYQVIDYDGSDWRANNSRGFFSDNFDQAAIHPDWTVMTGTWGINNGVLEQSDEAQTNS
                     NIYAPLAQNLSNKYLYNWSGKIDGTGNNRRAGFHFFCDDATLPNRNNSYFVWFRVDQS
                     VCEFYKVVNDVFTLENSVPMTVNAGQWYDWKVVYDRITGEMDVYQDNSFVGSWTDPAP
                     YSTGGYVSFRSGNSNWQINNFKVYRSRYSNAQVTVNVGNCPSCDIRYQNTNPSTPAGR
                     VKSVVRDSAGNFSAIAFVDVNIDWTAPDAIDTVNDGPAADIDLSTSPTDLAANWSSSA
                     DTNSGLARYYYALGTSPGDSDVVPWTSNWGYDTVALTGLPLVSGQWYYFSVMAENGAG
                     LRTQHFTSDGVLVDLTTGISSSYADGFSATAGPNPFTGNTTIAYNIPQNENVRIVLSD
                     GNGKRIELLNELQNAGSHSVSIDSGNLALASGMYFITFTCGTGVHTIPLIKK"
     gene            2159..3760
                     /gene="gldM"
                     /locus_tag="VFU15_04810"
     CDS             2159..3760
                     /gene="gldM"
                     /locus_tag="VFU15_04810"
                     /inference="COORDINATES: protein motif:HMM:TIGR03517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldM"
                     /protein_id="HEU4717127.1"
                     /translation="MAAGKLSPRQKMIGMMYLVLTALLALNVSKEILEAFVTVNNGLE
                     NTKKSFDKDISSLYAKFDEKKSIDPNRVMENWNRAQQAKKLSTDMNLFIENLKKDIMK
                     ETEGFKNKEEDTIHLAYIKSKDNYDTPTNILCGESEDGKNGQAAVLKKKLNEYQQSML
                     ALLSPEDQKALHLSIDTDDPKESGEFHTWEMKTFYHAPLAADVTLLSKIQDDVKGTEA
                     DVVDALLKETDSDIIPFDTVAARVIAQTNYVMLGEPYQADIFLAAFNKTLAPRIYIGQ
                     YDSITGKMIGTFDSVHVAGGLGKYSVNTTKEGIFTYEGVISMTTPKGAVRQFPFQSEY
                     IVARPSLNVSADNMNVFYAGLDNPVTVSVPGVPNEKVHAHIDNGTLTPLGNGKYMAKG
                     LTAGKAHISVSATLDNGEERNMGSTEFRVKQLPAPYVTVSGATPARTSASSICNSLGL
                     VCAYDPNFNFNARANIVSYSIDVYIGGRFLYSKEGVRGYQLPKEAKDAVCGAKKGTKV
                     SIYNITAVGADNHQIKAPDVLYTIQ"
     gene            3927..6182
                     /locus_tag="VFU15_04815"
     CDS             3927..6182
                     /locus_tag="VFU15_04815"
                     /inference="COORDINATES: protein motif:HMM:NF038133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor L domain-containing protein"
                     /protein_id="HEU4717128.1"
                     /translation="MVKNIRYILFALAALIPFYGFGQLNVNTAVTANQLISSLIGNGL
                     TVSNISLNCPAGAYGTFSNGNATNLGINNGILLTTGTAAGVPGANTSASDVSFCSGPV
                     YNDPDLVALDPSAVNDACILEFDIVPKCDSLTIRFVFGSEEYPTYVNSFNDLFGFFIT
                     GANPAGGNYTGLNIATLPNGQYVSINNVNNGNTNTGPCVNCAYYVDNTNGTTIQYNGM
                     TTVLTSSVWLVPCTTYHFKIAIADAIDCILDSGVFVDFLQCSTAFSVQQNSSPDQCNS
                     CVGSATVNVTGGIPPYSYQWLPTGGNGATANNLCAGTYSVLVMDQSSCGIPDTEIVNI
                     SNVGSLAATVQQVNVSCNGGCNGTLDVTPQGGTAPFTYTWNPNVSNTNSATGLCAGQY
                     VVDVADANGCSSTYTYNITEPAALSLALNGNNNVCAGVSTTINAAPAGGTGPYTVSWS
                     NNLPNGNSATVAPNATTIYTATVTDSAGCTAQQTFTVTVNPSPVANFSTGPADCAPAF
                     VAFTDSSVSATTYSWDFGDPLSGSNTSALQNPTHLYSTAGTYNVSLVVTGPGGCSDTL
                     IIPNAVTVSPQPVAAFTVLNPVVSENDPTVIFTDQSQSGTGCVFYFGDGDSLNTCNFG
                     TIDHAYPGPGTYQAMMIVSDATGCADTISMTIVVENESSFYVPNAFTPNGSGQNDVFY
                     AYGSNIDNFDMLVFDRWGNLLFESHDINKGWDGTYKNQPCEEDVYVWKITYTNSHGEK
                     KKAIGSVSLLK"
     gene            6231..>7729
                     /locus_tag="VFU15_04820"
     CDS             6231..>7729
                     /locus_tag="VFU15_04820"
                     /inference="COORDINATES: protein motif:HMM:NF024962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprB repeat-containing protein"
                     /protein_id="HEU4717129.1"
                     /translation="MRLPCFPLRFLLPLFVWMLPHDVQAQLCLSADAGPGDTVCAGQC
                     TTLSSQISGTRSTATYSCSTTPYSPFAFTGGNPVLVNLDDLWSPVVSLPFCFEFYGTV
                     YNQLIIGSNGVASFDISQANGNCPWTINTAAPNNGLPMSSIMAPFHDIDPALPVTSNA
                     TSINWNVYGTAPCRSFVINWNEVAMYGNGCGSLVSTSQAVLHETTNVIDIFIGNSSVC
                     PAWNGGAAIEGIQNANGTLACVAPGRNYPAQWAAAFDGMRFSPAGAPGYTIQWLDPSG
                     TVLGTAPTQAVCPTQTTTYTLNVVNTGCAGTLSLTDSTTVYVSNSPLAATYTAVYPNC
                     FGNCNGSIDVTPTAGIGPFTYSWLPGGSTQPSVSNLCTGVYACTITDATGCSIVVAVN
                     LQTPPPFTLTGQCTPTLCNASTGTAGVLVSGASGPYTYLWSTGDTTATLSNLAAGIYY
                     VTVTDSAGCQDGITISVLMNGLQLASQLTPLLCDGDCNASLSVTPLNGIP"
BASE COUNT         1905 a         2166 c         2008 g         1650 t
ORIGIN      
        1 gacaacaaca tcgatcccct gcggaccggc tggtggccgt ggctgcacac cacctattac
       61 aaccagagcg gaattccggg tgcctgcacg aaggtcaaag gacacatgca tttcccgaac
      121 cagacgcata atgatccggg acccaactgg gactgggatt attactaccg tcttatcaat
      181 aacaatcctc ctgccgccac gcagcttacc gctgcttcgg gaaattttta cgattcgggc
      241 ggggccagcg caaattattc tgatgacgaa aggagcacct ggacaatcgc gcctccgaat
      301 gcaacttcag tgacgatctc gttcacgagt ttcgatgtgg aaaacacctg ggattattta
      361 ttcatttacg acggcaccga tatcaatgct ccgctgatcg gttattatac cggcacgagc
      421 aatcccggca cgatcacttc gagcggaggt gcgctgacgt tcgaattccg ttcggattgc
      481 tccaccaacg ccacaggctg ggcggcaacg tggtcgagca cgatcaacaa cacgagcacc
      541 gatatcattc cgccgaccac ggcgctttcc atcaacggaa catggcagac aaccgatttc
      601 cccgcaacgt tcagcgattc cgacaacgtg ggcggaagcg gtcttgaaaa aagtttttac
      661 caggtgatcg attatgacgg cagcgactgg cgtgcaaata attcacgcgg atttttcagc
      721 gacaatttcg accaggccgc cattcatccc gactggacag tcatgacggg aacatggggg
      781 atcaacaacg gcgtgctcga gcagagcgat gaagcgcaga cgaacagcaa tatctatgcg
      841 ccgcttgcgc aaaacctttc gaacaaatac ctctacaact ggtcgggaaa aatcgacggg
      901 acggggaaca accggcgcgc aggattccat ttcttctgcg acgacgcgac cttgcccaat
      961 cgcaacaatt cctatttcgt gtggttccgc gtggatcaga gcgtatgcga attctacaaa
     1021 gtggtgaacg atgttttcac gctggaaaat tcagttccga tgaccgtgaa cgcgggacaa
     1081 tggtacgact ggaaagttgt gtacgaccgc atcacgggcg agatggacgt ataccaggat
     1141 aactcgttcg tcggaagctg gacggatccc gcaccttatt ccacgggcgg ctacgtttca
     1201 ttccgcagcg gcaacagcaa ctggcagatc aataatttca aagtttaccg ctcgcgttat
     1261 tccaacgcgc aggtcacggt gaacgtgggc aactgtcctt cgtgcgacat ccgttaccag
     1321 aatacaaatc cctcgacgcc ggccggaagg gtaaaatctg tcgtgcgtga ttccgccgga
     1381 aacttttccg ccattgcgtt cgtcgatgtg aatatcgact ggacggcacc ggatgcgatc
     1441 gacacggtca atgacggccc ggctgcggac atcgatcttt ccacctcacc aacggatctc
     1501 gcggcgaact ggtcgtcttc cgcggacacc aattccggcc ttgcgagata ttattacgcg
     1561 ctcggcactt cgccgggtga ttcggatgtc gttccgtgga cgagcaactg gggctacgac
     1621 actgttgcgc tgaccgggct tccgctcgtg agcgggcaat ggtattattt ttccgtgatg
     1681 gcggaaaacg gcgccgggct gagaacacag catttcacga gcgacggcgt gctggtggac
     1741 ctgaccacag gtatttcctc ttcctacgcg gatggatttt cagcgactgc aggtccgaac
     1801 ccgttcacgg ggaacacgac catcgcgtac aacattccgc agaatgaaaa tgtacggatc
     1861 gttttatccg atggaaacgg gaagcgcatc gagctgctga acgaactgca aaatgcggga
     1921 agtcattccg tgagcatcga ctccggcaac ctggcgctcg ccagcggcat gtacttcatc
     1981 acgttcacct gcggaaccgg cgttcacacc attccgctca ttaaaaagta agcagccgat
     2041 ttatttattt tccggggccg gcacttcgcc ggtctttttt tttgcggcct gtatcttttt
     2101 caaaacaccg gcattaggac ggcaaattca aacaagtcca acctaaaaaa cattcaccat
     2161 ggcagccggt aaactttctc cacgccagaa gatgatcggg atgatgtacc tggttcttac
     2221 tgcgctttta gcactgaacg tttcgaaaga gatcctcgaa gcattcgtta ctgtgaacaa
     2281 cggtctcgag aacacgaaaa aaagcttcga caaggatatt tcctcgcttt acgccaaatt
     2341 cgacgagaag aaaagcatcg acccgaaccg cgtgatggaa aactggaacc gtgcgcagca
     2401 ggcaaaaaaa ctttcgaccg acatgaatct tttcatcgaa aacctgaaga aggatatcat
     2461 gaaggaaacc gaaggtttca aaaacaaaga agaagacacg atccacctcg cctacatcaa
     2521 atcgaaagac aattacgata cgccgacaaa tattctctgc ggtgaatcgg aagatggaaa
     2581 gaacgggcag gcggccgtgc tgaaaaagaa actgaatgaa taccagcaat cgatgctcgc
     2641 attgctttcg cccgaagacc agaaagcgct gcacctgagc atcgataccg atgatccgaa
     2701 agaaagcggg gaattccaca cgtgggaaat gaaaacattt taccacgccc cgcttgcggc
     2761 ggatgtaacg ctgctttcga aaatacagga cgatgtgaag ggaacggaag ccgacgtggt
     2821 ggatgcattg ctgaaagaaa ccgattccga cattatcccg ttcgacaccg ttgcagcacg
     2881 cgtgatcgca cagacgaatt acgtgatgct cggcgaacct taccaggcgg atattttcct
     2941 tgcagctttc aacaaaacac tggcaccgag aatttacatc gggcagtacg attcgatcac
     3001 cgggaaaatg atcgggacgt tcgacagcgt gcacgtggcc ggcggcctcg ggaaatattc
     3061 cgtgaacacg acgaaagaag gaattttcac ctatgaaggc gtgatcagca tgacaacacc
     3121 gaaaggcgcc gtgcggcagt tcccgttcca gtcggaatac atcgtggcgc gtccttcgct
     3181 caatgtcagc gcggacaaca tgaatgtttt ttatgcgggc ctggataatc ctgtaactgt
     3241 ttccgtaccg ggcgtgccga acgaaaaagt tcatgcgcac atcgacaacg gaacactgac
     3301 accactgggc aatggcaaat acatggccaa aggactgacg gccggaaagg cgcacatttc
     3361 cgtttcggcc acactggaca acggtgaaga acgaaacatg ggctcgaccg agtttcgcgt
     3421 gaagcagctg ccggcaccgt acgtcaccgt ttcaggcgca acgcctgcgc gcacatcggc
     3481 ttcttccatc tgcaacagtc tcggactcgt atgcgcgtat gatccgaact tcaatttcaa
     3541 tgcccgtgca aatatcgtct cctacagcat cgacgtttac atcggcgggc gttttttgta
     3601 ttcgaaggaa ggagtcagag gataccaact cccgaaggaa gcaaaggatg ccgtatgcgg
     3661 cgcgaagaaa ggcaccaaag tttccattta caacatcacc gccgtcggag cagacaatca
     3721 ccagatcaaa gcgcccgacg tattgtatac catccaataa ccccacgcac aaagagcggt
     3781 acgtgcgtgt ttgcgtggaa cgggccggga aatctattcc cggtccgttc gtgctgttac
     3841 gggaaagtta aattgagcta tatttgatat ggcaacacaa tcgcccggtt tcccggccat
     3901 cttccggcgc gattcaatcg acacagatgg tgaaaaacat acgatacatt ctcttcgcgc
     3961 ttgcagcgct gatcccgttt tacggtttcg ggcagctcaa cgtgaacacg gccgtaaccg
     4021 ccaaccagct tatttcatcg ctcatcggca acgggctgac ggtttccaat atttcgctca
     4081 attgtcccgc cggcgcgtac ggaacatttt ccaacgggaa cgcgacgaac ctcggcatca
     4141 ataacggtat tcttcttacg accggaacag cggccggtgt gccgggcgcc aatacatccg
     4201 cttcggacgt gagcttttgt tcaggaccgg tttataacga tcccgatctt gtggcactgg
     4261 atccgagcgc ggtgaatgat gcgtgcattc tcgaattcga tattgttccc aaatgtgatt
     4321 cgctgacgat acgttttgtg ttcggttcgg aagaataccc gacgtatgtg aacagcttca
     4381 atgatctttt cggttttttc atcaccggcg ccaacccggc cggggggaat tacacgggac
     4441 tcaacattgc cacgctgccg aacgggcagt acgtttccat caacaacgtc aacaacggga
     4501 acacgaatac agggccgtgc gtgaactgcg cgtattatgt cgacaacacg aacggaacaa
     4561 cgatccagta caacggcatg acgaccgtgc tcacctcgag cgtgtggctg gtgccatgca
     4621 cgacttatca tttcaagatc gccattgcgg atgcgatcga ctgcattctc gactcgggcg
     4681 tgtttgtcga tttcctgcaa tgcagtacgg cgttctccgt tcagcagaac tcttcgcccg
     4741 atcaatgcaa ctcctgtgtc ggcagcgcaa cagtgaatgt gacgggagga attccacctt
     4801 attcgtacca atggctcccg accggcggca atggcgcaac agctaacaat ctttgcgcgg
     4861 gaacgtattc ggtccttgtc atggatcagt cttcatgcgg aattccggac acggagatcg
     4921 tcaacatctc aaacgtaggt tcgctcgcgg ccactgttca gcaggtgaat gtttcctgca
     4981 acggcggatg caacgggacg ctcgacgtaa cgccgcaggg aggaaccgcg ccgttcacct
     5041 acacatggaa cccgaacgtg agcaacacga attcggcgac aggtctgtgc gccgggcaat
     5101 atgtcgtcga cgtcgcggac gcgaacggat gctcgagcac gtacacgtac aacatcacag
     5161 aacccgctgc actttcgctt gcgctgaacg ggaacaacaa cgtctgcgcc ggcgtgagca
     5221 cgacaataaa cgctgctcct gcaggcggca cgggtcctta tacggtctcg tggagcaaca
     5281 atttaccgaa cggaaattcc gcaacagtcg cgccgaatgc aacgacgatc tacacggcaa
     5341 ccgttaccga cagcgcgggc tgtacggcgc agcaaacgtt caccgtcaca gtcaacccgt
     5401 cacccgtggc gaatttcagc accgggcctg ccgattgtgc acctgccttc gttgcattca
     5461 cggacagttc cgtttcagca acaacttatt cctgggattt cggcgatccg ctttccggct
     5521 cgaatacatc cgcgctgcag aacccgacac acctgtattc aacagcagga acctataacg
     5581 tttcgcttgt cgtcaccggg ccgggcggat gttcggacac gcttattatt ccgaatgcgg
     5641 ttaccgtttc accgcaaccg gtggctgcgt tcactgtttt gaatcctgtt gtcagcgaaa
     5701 atgatcctac cgtgatcttc acggaccagt cgcagtcggg caccggctgt gtattctatt
     5761 tcggcgacgg cgattcgctg aacacctgca atttcggaac gatcgatcac gcgtacccgg
     5821 gaccgggcac gtaccaggcg atgatgatcg tcagcgacgc aaccgggtgt gcggatacga
     5881 tctcgatgac gatcgtggtg gagaatgaat cctccttcta cgttccgaat gcgttcacac
     5941 caaatggcag cgggcagaac gatgtgttct atgcgtacgg ttcgaatatc gacaacttcg
     6001 acatgcttgt gtttgaccgt tggggaaatc ttcttttcga aagccatgac attaacaaag
     6061 gatgggacgg cacgtacaag aatcaaccct gtgaagaaga tgtttacgtc tggaagatca
     6121 cgtatacgaa ttcgcacggc gagaagaaaa aagccatcgg atccgtttcg ctgctcaagt
     6181 gatttatcac agaatgaaaa acaaaaccgg gagttttgcc cgggcaacaa atgcgcctcc
     6241 cgtgttttcc cctgcgtttc ctgcttccgc ttttcgtgtg gatgcttccg catgacgtgc
     6301 aggcgcagct atgcctttcc gccgatgccg gtcccggcga taccgtatgc gcaggacaat
     6361 gcacaaccct ttcgtcgcag atcagcggaa cacgaagcac agcaacatac tcatgttcga
     6421 caacaccgta ttcgccattt gcattcacgg gcggaaaccc ggtactcgtg aatctcgacg
     6481 acctctggtc gcctgttgtc agtcttcctt tctgctttga attttacgga accgtttaca
     6541 accagctcat catcggatcg aacggagttg catcgttcga tatttcacag gcaaacggaa
     6601 attgtccctg gacgatcaat acggccgcgc ccaacaacgg gctcccgatg agcagcatta
     6661 tggcgccctt ccatgatatc gatccggcac ttcctgttac gagcaatgct acttcgatca
     6721 actggaatgt atacggaact gcgccctgcc gctcgttcgt catcaactgg aatgaggtgg
     6781 cgatgtacgg gaacggatgc ggctcgcttg tttcaacctc gcaggctgtg ctccatgaaa
     6841 caacgaacgt gatcgacatt ttcatcggca acagttccgt gtgtcctgcc tggaacggcg
     6901 gagctgcaat tgaaggaata cagaatgcga acggaacact tgcatgcgtc gcgccgggaa
     6961 gaaattaccc ggcacagtgg gcagccgctt tcgacggcat gcgcttcagt ccggccggcg
     7021 caccgggcta caccatacaa tggctggatc catccggcac tgttctcggc acggcaccca
     7081 cgcaggctgt ttgcccgacg cagacaacca cgtacacgct gaacgtcgtg aacaccggct
     7141 gtgcaggaac attatcactt acggatagca caaccgtata cgtgagcaat tctccgcttg
     7201 cagccacgta cacggccgtt tatcccaatt gctttggcaa ctgcaacggc agcattgacg
     7261 tcacgcctac tgcaggaatc ggtccgttca cctattcatg gctgccgggc ggatcgacgc
     7321 agccgtcggt cagcaatctg tgcaccggcg tatacgcatg cacgatcacc gatgcaaccg
     7381 gctgttcgat cgtcgtcgcc gtaaacctgc agacgccacc gccattcacg ctcaccgggc
     7441 aatgcacccc gacgctttgc aatgcaagca cgggaacggc aggtgtgctg gtaagcggcg
     7501 cttccggacc gtacacctac ctgtggagca cgggcgatac aacagcgacg ctaagcaacc
     7561 tcgcggcggg aatatattat gtcacggtga cggactcagc cggctgccag gatggaatta
     7621 cgatcagtgt gctgatgaac ggacttcagc tcgcgagcca gctgacgcca ctgctctgcg
     7681 atggcgactg caacgcatca ctgagcgtta caccgctcaa cggtattcc
//
LOCUS       DASTSF010000166         2157 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_500853,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000166 DASTSF010000000
VERSION     DASTSF010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2157
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_500853"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..966
                     /locus_tag="VFU15_04825"
     CDS             <1..966
                     /locus_tag="VFU15_04825"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEU4717130.1"
                     /translation="DSTVTTNLTVLPVIVSSQALSVCNGGSVTVGTHTYTTSGIFTDT
                     LSAVNGCDSVITTDLIVLNAIVNNLSAFICDNDSIYVGGSWQTASGIYHDTLVSAGGC
                     DSVVVTTLTVNPGFVTSQSVTICPHDSILLGGSWQHLAGIYYDTLHSQIGCDSVLVTT
                     LSVNNVDTSLTVHSDTLVANAAGTTFQWIDCSTGAFIAGATDAVFVPSVSGTYAVIIT
                     ENGCSDTSSCYSVIIDGISGPGTDNMFTVYPNPVKDVLMISMTGDYKSEVHCRIFNVI
                     GEQVLDGTYQNAPVITLDLQSFADGTYFVEISSENKTSVVKVIKN"
     gene            complement(1157..1954)
                     /locus_tag="VFU15_04830"
     CDS             complement(1157..1954)
                     /locus_tag="VFU15_04830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717131.1"
                     /translation="MAYLKKLFEQFFSLVILILLEIVIAYFIQDSDHFGEFMLVSLVL
                     DVFAFIGSFMVAGSHSKAIRKYRKKLGVHSYTPLFYCIPLGSDNGRIFHAAGLFTKNS
                     LIICKVSNDISHNVVSDFWESIEEDLIHIPFSSIREIGTEQAAHENYEDERRKAYLKD
                     LVRNQTIGRLLRDKQKTIVFTAFIYIVYMDANSQEKIIVFGIGENTSGLGLSALAEPA
                     LAGWINNTMAVAEVAAELADEGNVKEEVHMARKVAGNLIARVRSAQA"
     gene            complement(1972..>2157)
                     /locus_tag="VFU15_04835"
     CDS             complement(1972..>2157)
                     /locus_tag="VFU15_04835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717132.1"
                     /translation="FDRSLRARTEPALLYNMDTLPPDRQVQQQKNQRSNLLIFMKQRR
                     HLRKPGTVFKFSFPATH"
BASE COUNT          539 a          518 c          549 g          551 t
ORIGIN      
        1 gacagcacgg tgacaaccaa tctcacggtt cttcctgtga tcgtttcctc gcaagcactt
       61 tctgtatgca atggcggaag cgtaacagtc ggaacgcaca cgtacacgac aagcggaata
      121 ttcacggata cgctttctgc cgtgaatggt tgtgacagcg tgatcacgac cgatctcatt
      181 gtgctgaacg cgatcgtcaa taacctgtct gcgttcattt gcgataacga cagcatttac
      241 gtgggcggat cgtggcagac agccagcgga atttatcacg atacattggt ttctgcagga
      301 ggctgcgaca gcgttgttgt aaccacgctt accgtgaacc ctggatttgt aaccagccag
      361 tcggtaacta tttgtcccca tgacagcatc ctgcttggag gatcatggca gcacctggcg
      421 ggaatttatt acgatacatt gcattcgcaa atcggctgcg acagcgttct cgtaacaaca
      481 ctgtcggtga acaacgtgga tacttcactt accgttcatt ccgacacact cgtggcgaac
      541 gcagcaggaa caacattcca gtggatcgac tgcagcaccg gggcattcat tgcaggtgca
      601 actgatgcgg tgttcgtgcc gtccgtatca ggaacctatg cggtcatcat tacagagaac
      661 ggatgcagcg atacgtcatc atgctacagc gtaatcatcg acggcatttc cggtccgggc
      721 acggataaca tgtttacggt ttatcccaac ccggtgaaag acgtgctgat gatctcgatg
      781 acgggtgact ataaatccga agtccattgc cggatattca acgtcatcgg ggagcaggta
      841 ctggacggga cgtatcaaaa tgcgccggtc atcacgctcg atctccagag ttttgctgac
      901 ggaacttatt tcgtcgagat cagctcggag aataagacat ctgttgtgaa agtgataaaa
      961 aattaaacga agagcagagg tgccgggtcg caaaacgccg gaatgcaggc gggggaactg
     1021 attggcataa gctacaggtg cgaaagcggg tcacggaaaa tggcccgctt ttgcgtttga
     1081 ggaactggcc ggctttcttc ggaataccgg gattcccggg ggccgcgttt tttgttccgg
     1141 acatcgtatg aaattctcat gcctgcgcag atcttaccct ggcgataaga ttccccgcca
     1201 ctttccttgc catgtgcact tcctctttca cattaccttc atccgctaat tccgcagcga
     1261 cctctgcaac tgccatggtg ttattgatcc agccagccag ggcaggctct gcaagcgcgg
     1321 ataatccgag gccgcttgtg ttttccccga ttccgaaaac aatgattttc tcctggctgt
     1381 tcgcatccat gtaaacgata tagatgaagg cggtaaatac gatggttttc tgtttatcgc
     1441 gcaggagacg gccgatggtt tggttcctta ccaggtcctt caggtaagct ttccgtcttt
     1501 cgtcctcgta gttttcatgc gcagcctgtt ccgtgcctat ttccctgatc gaagagaacg
     1561 ggatatggat aaggtcttct tcaatgcttt cccagaaatc gctgacaaca ttgtgcgaga
     1621 tatcattcga caccttgcag atgatcaatg aattttttgt gaaaagaccg gcggcatgaa
     1681 aaattcttcc attgtcggat cccagtggaa tgcagtaaaa gaggggggta tacgaatgca
     1741 cgcccagttt tttcctgtac tttctgatcg ccttcgaatg ggagcccgcc accataaagg
     1801 atcctataaa agcgaaaacg tccagcacaa gacttacgag cataaattca ccgaagtggt
     1861 cggaatcctg tatgaaataa gcgatcacga tttccaggag tatcaggata accagggaaa
     1921 aaaattgttc gaacagcttt ttcaggtaag ccatagaagt cacggtttag gttaatgcgt
     1981 ggcgggaaaa gaaaatttga atactgtgcc tggtttgcgc aggtgccggc gctgtttcat
     2041 aaaaataaga agattacttc tttgattttt ttgttgctga acctgcctat cgggcggcaa
     2101 tgtatccatg ttgtacaaaa gagccggttc tgtccgggcg cgtaaggatc tgtcaaa
//
LOCUS       DASTSF010000167         2355 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_596385,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000167 DASTSF010000000
VERSION     DASTSF010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2355
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_596385"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="VFU15_04840"
     CDS             complement(<1..189)
                     /locus_tag="VFU15_04840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717133.1"
                     /translation="MKRILSFLFLLSVTGLFAQAPPQGINYQAVARNASGAELTNTTL
                     SVRISIYSDAAATQLVFQE"
     gene            complement(193..699)
                     /locus_tag="VFU15_04845"
     CDS             complement(193..699)
                     /locus_tag="VFU15_04845"
                     /inference="COORDINATES: protein motif:HMM:NF039201.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEU4717134.1"
                     /translation="MVKILRCAVFIFFSFSATAQTASPEVISSGGGSADASLLFNYSA
                     SSRGTSPGMFSAGTTATQGFRQAQDISASVRDEDSTGASSLYPNPSDGTVWIGLDLVQ
                     PSVVSVEIFNSLGQVVLVQKCVVAAGSQKIQVDLSSLDNGIYFVQCLATSDDEVSMKQ
                     TFKVTLTR"
     gene            1008..>2355
                     /locus_tag="VFU15_04850"
     CDS             1008..>2355
                     /locus_tag="VFU15_04850"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015546699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HEU4717135.1"
                     /translation="MIETENVALQEQLQKFFGFNKFKGQQEAIINSVLEGKDTFVIMP
                     TGGGKSLCYQLPALTSEGTAIIVSPLIALMKNQVDAIRNFGTDDGIAHFLNSSLTKAE
                     IQKVKKDIVSGKTKLLYVAPESLTKEDNVAFLRDIRISFFAIDEAHCISEWGHDFRPE
                     YRRLRPIIEAIGTVPIIALTATATPKVQQDILKNLGMTQANVFKSSFNRSNLFYEVRP
                     KVNVVKEIIRYIKKNSGKSGIVYCLSRKKVEELAETLKVNGIKALPYHAGLDAATRAR
                     TQDDFLMEEIDVIVATIAFGMGIDKPDVRFVIHHDIPKSLEGYYQETGRAGRDGGEGN
                     CLTFYSYDDILKLEKFMKGKPVAEQEIGKQLLLETVAYAESSACRRKVLLHYFGEGYD
                     ADNCDNCDNCRNPKTKTDATDDIGLVIETALEIKEKFKDKHIIQVLQGITTNTIKSY"
BASE COUNT          669 a          585 c          615 g          486 t
ORIGIN      
        1 ttcctggaac acgagctgcg tcgccgccgc atcgctgtag atggaaatgc gcaccgagag
       61 agtcgtgttc gtcagctccg cgcccgatgc gttccgcgcc accgcctggt aattgatgcc
      121 ctgcggggga gcctgcgcaa acagaccagt tacagaaaga agaaacagga aagacaggat
      181 gcgtttcatg atttacctgg ttaatgtgac tttgaaagtt tgcttcatgc tgacctcgtc
      241 atcggaagtg gcaaggcatt gcacgaaata aataccgtta tcgagagaag aaagatccac
      301 ctggattttc tggctgccgg cagcgacgac acatttctgc acgagtacca cctggccgag
      361 ggaattgaaa atttccacgc ttactacgga cggctgaaca agatcaagcc cgatccacac
      421 agttccgtcg gatggattgg gataaagcga ggaagcgccg gtggaatcct cgtcgcggac
      481 agaggcggaa atgtcttgcg cctgccggaa cccctgcgtc gctgttgtcc cggcggaaaa
      541 cattccgggc gatgttccac ggctgctcgc ggaataattg aacaacaggg atgcgtccgc
      601 agatcctccg ccggaagaga tgacttccgg tgaagcggtt tgtgctgtag ctgaaaaaga
      661 gaaaaagata aaaactgcgc agcgtaagat ttttaccatg tcggaaattt aggaaaagcc
      721 cgtggagaac ggaaagaatc cgcggatttc gccaaatttt ttgcgggccc ggaatgactt
      781 gcgcaaaagc ggcgcggctt tgttttttat cgctgaagac agggagggaa aaagaacggt
      841 ttacgaataa aaaaagagtt tttcccggtt tatcggcgaa aaatttccga tcgtagaaaa
      901 gtgaaaaaaa ctaacgaaaa tctcacgagt aaacgaaaaa ttcattatat ctttactcag
      961 atcacgattg aaaaaattat ctcccaacga cagaaaattt attagaaatg attgaaactg
     1021 agaatgttgc gctccaagaa caacttcaga agtttttcgg attcaacaag ttcaaaggac
     1081 agcaggaggc gatcatcaac agtgtgctgg aaggcaagga tacgtttgtc atcatgccta
     1141 cgggcggcgg aaaatcactc tgctaccagc tcccggctct caccagcgaa ggaacggcca
     1201 tcattgtttc cccgctcatc gcgcttatga aaaaccaggt cgacgccatc cggaatttcg
     1261 gcacggatga cgggatcgcg catttcctga acagttcgct taccaaagca gaaatacaaa
     1321 aagtaaaaaa agatatcgtc tccggtaaga caaagctgct gtacgtggca cccgaatcgc
     1381 ttaccaagga agataacgtc gcctttctgc gcgatatccg catttctttt ttcgcgatcg
     1441 acgaagcgca ctgcatttcc gaatgggggc acgacttccg cccggaatac cgccgtctcc
     1501 gcccgatcat tgaagccatt ggcacagtgc ccatcattgc gcttaccgcc actgccacgc
     1561 cgaaagttca gcaggacatc ctgaaaaatc tcggcatgac gcaggcgaac gttttcaaat
     1621 catcgttcaa ccgctcgaac cttttttacg aggtgcggcc gaaggtgaac gtggtgaagg
     1681 agatcatccg gtatatcaaa aagaattccg gcaaatcggg catcgtgtat tgcctgagcc
     1741 ggaaaaaagt ggaggagctg gcggaaacac tcaaagtgaa cggcatcaaa gcgcttccct
     1801 atcacgccgg gctcgacgcg gccacgcgcg cgcgcacgca ggatgatttt ctcatggaag
     1861 agatcgacgt gatcgtcgct acgatcgctt tcggaatggg catcgacaag ccggatgtgc
     1921 gtttcgttat tcaccacgat attcccaaat cgctggaagg ctattaccag gaaacaggtc
     1981 gtgccggccg cgacggcgga gaagggaatt gtcttacgtt ctacagctac gacgacatcc
     2041 tgaagctgga gaaattcatg aaaggaaaac ccgttgcaga acaggagatc gggaaacagc
     2101 ttctgctgga aaccgtggcc tacgccgaat catccgcctg ccgcaggaaa gtgctgctgc
     2161 attatttcgg ggaaggctac gatgcggata attgcgataa ctgcgataat tgccgcaacc
     2221 cgaaaacgaa aacagatgca acggacgaca tcgggctggt gatcgaaacc gcgctcgaga
     2281 tcaaggaaaa attcaaagac aagcatatca tccaggtgct gcagggcatc accacgaaca
     2341 cgatcaaatc ctaca
//
LOCUS       DASTSF010000168        14228 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1423400,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000168 DASTSF010000000
VERSION     DASTSF010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 14228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14228
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1423400"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1288)
                     /locus_tag="VFU15_04855"
     CDS             complement(<1..1288)
                     /locus_tag="VFU15_04855"
                     /inference="COORDINATES: protein motif:HMM:NF019284.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717136.1"
                     /translation="MKTLLAFFLPLVFLLPAHAQEAGNPLRYGKKSGRIVSGTGYAVT
                     KGHVKKHMAEGRWKTVSAANGNVLQVHRYSKGYYDGEQDYYNEDLVAGKSLLVTKSFY
                     RMGRKDSAWISYNSKGAVIWISHYHLDTLMSDSSWSADGKLLMAKSYRADGFVTTQCF
                     WPNGRVRSASTLNGTKHEGWVIDNPDSGWKDSLPQFYSHYSGGKKDGGERARLSGGYS
                     MTCNWKSGLYDGEFRLFYPGGKIEFSGQFRDGIKTGAWKYYSPSGVLLQTTWFHNGCF
                     LPAGNSTREYDTLPQPPPDSVYTFDGNGRLSCISRSALRPEEFTGYLSETFLARTHEQ
                     FFDTTGYIKEEGDLSGTRKDGTWKTFYPGGKLRSSLDYVSGTANGTYTVFYPGGKKMI
                     TAEVNDFLAVKAVFYGKDGKPLALSSPKVDEMSGQWF"
     gene            complement(1409..2005)
                     /locus_tag="VFU15_04860"
     CDS             complement(1409..2005)
                     /locus_tag="VFU15_04860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717137.1"
                     /translation="MRLLLTLVMAGVFSFAAKAQEPGHEITFTSFSAIAHGSHVLLSW
                     QAESKDVISYDLQKSKNGSDYDIFGTVQAGDLAGMEFMETDFAPAKGLSWYRLRMTNM
                     DGSVTYSNTVPVKYDDNMEPVSPVAATNSYNTAPSDSSVLIIVQNAAGEEFYSMVDIT
                     GKGNPMECADLDPALSSGTYTIVGCSRQEFYSRQVLVK"
     gene            2176..3192
                     /locus_tag="VFU15_04865"
     CDS             2176..3192
                     /locus_tag="VFU15_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HEU4717138.1"
                     /translation="MKLPFFAFTALFPFLLAAQDKSGLHVTDTFHIRSDGGWDYIAVD
                     HDLNRLYVSHGTQVNILNETTGDSVGIIPNTTGVHGIAFAPAFGKGYTSNGKLGTVTV
                     FDLKTNSVLKQVHVGENPDAIMFDDYSKRIYTCNGHSHDMSIIDPQKDTLVGTIPLGG
                     KPETAVSDGNGKLFVNIEDKSEIAVIDLAKDSVIARWKTGSGEEPSGLAIDRKNGILF
                     SGCSNKLMIVMDCKTGNVIAELPIGDGCDGTAFDPGTMNAFSSNGDGTLTVIHEDSPK
                     KFTVTGNVATKRGARTLCVDLQTHKIYLPTAQLGDTPPATADNPRPRPKKIPGTFQVI
                     VVGK"
     gene            3231..4331
                     /locus_tag="VFU15_04870"
     CDS             3231..4331
                     /locus_tag="VFU15_04870"
                     /inference="COORDINATES: protein motif:HMM:NF040322.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEU4717139.1"
                     /translation="MKNRIFTAALFLLLSLSLIAQKPVARIAFWNVENLFDTINDPNI
                     NDEEFLPSAKDHWTSDRYLAKINHLAQGILAIGDGAGPDILGMAEVENEGVLKDLTQK
                     TDLKKQKYGIVHYDSPDKRGIDVAMLYKKSKFKVLDSRPVNVSVPADTFFTRDILVVK
                     GILNKTDTVWIIVNHWPSRRGGAEASESKRMIASRKLQGILDSIMKMNPRSLVLAMGD
                     FNDEPTNNSIMNLKCNDWLDPAMQQKLDTLNKIIGESIDNQGAGWMMAPSQNCFHDLM
                     DSLKDAGDGSYHYRKEKDMIDQFLVNGNLYGTGYRITVHSAHIFKADFLTGENYKSDP
                     PGPLHTYAGSRYIGGYSDHYPVYADVVTTGKK"
     gene            4462..6414
                     /locus_tag="VFU15_04875"
     CDS             4462..6414
                     /locus_tag="VFU15_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017731373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HEU4717140.1"
                     /translation="MNSDPKEELRRFVELLSTEKDEDYRQYIAQFERCSITERRKNGV
                     TWYPVRILSEEMGAGDYISIEVERTQGMKELHQFGGGKMVEIFSNTGDNGSETWKISG
                     TIRNVWGDRMRISFMTDELPGWTERGKLGLNLLFDENSYREMLIALQKVIDAGDDRES
                     RRLAKLRDVFYGLEKAEFEKEDTNLVLPGLNKSQNEAVRHIIAAKEVALVHGPPGTGK
                     TTTFIQAIRHTLMHEKQVLVCSPSNIAVDLLTEKLVQQGINVLRLGNPARVSEEVLNN
                     TLDAKMIAHPNYKELKEYRKRSEEFFAMAKKYKRNFGHAEREQRNLLYNEARQLVKEA
                     TALEDYIFWEQFGNAQVIACTPVVSAGKMLRDKKFRTVFIDEAGQALEPMSWIPITKA
                     ERVIFAGDHLQLPPTVKSKKAEDGGLKFTLFERLMRTQPHASVILRTQYRMNEKIMQF
                     SNRMFYGNKLEADVSVKDNVLSFDGEDDLLNRPVDFIDTAGCGFSEEQNPESLSYSNP
                     EEANLLLRYLERVLDQYEKMPDDSLRIGIIAPYRQQTEWLAQQLEEREWKKNSRHAFS
                     VRTVDGFQGQERDIIAISLTRSNDNGEIGFLADTRRMNVALTRAKKKLIIIGDSATLA
                     NHSFYKSFLDYVEEIGAYRSAWEMME"
     gene            complement(6452..6907)
                     /locus_tag="VFU15_04880"
     CDS             complement(6452..6907)
                     /locus_tag="VFU15_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU4717141.1"
                     /translation="METLKYKVITSVKQYNRYCRMLEELVFAEGKKTKEQKDEIALLT
                     LLVGKWDELHRNIAFRQLTPVELLLSLMSDHGMKAKDLARLLGVSKGYVSGILNYKKG
                     LSKEVIRRLAEYFSLSQEAFNRPYTLKSVPGRRLRNSPGLRKRRTAAVL"
     gene            complement(6916..7248)
                     /locus_tag="VFU15_04885"
     CDS             complement(6916..7248)
                     /locus_tag="VFU15_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HEU4717142.1"
                     /translation="MKVHLVKEQTIRRYIEEQARGRSSFNAWLSKVFLADWEKPEDIR
                     KTFASADFLGNGSSRIVFDIGGNNYRMIAKYAFGESEVHLFVCWIGTHAEYDKLCDSG
                     EQYIRSDY"
     gene            complement(7351..8973)
                     /locus_tag="VFU15_04890"
     CDS             complement(7351..8973)
                     /locus_tag="VFU15_04890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717143.1"
                     /translation="MQAQDLGRIGKKDMLTVSGGMNYNAVFYDAQGMQNRRDPFTWYF
                     NGNIDMNFLGVDLPFTYSYTNNHGTYTQPFNMQSCHPHYKWATAHIGTTAMNFSGYTL
                     SGRIFTGAGMELKPANWYVGAMYGRFSKADEGDAAVSFENAAFRRTGCAAKMGYDKNG
                     NALSATFFSAKDDAASLAFIPPEAGISPMENTAMSVSGKITLFRIFHVESEMALSGIT
                     KNSLSPVVTTGFSGLEKWMMTTHTTTAFFHAWKMSAGYTGKIFALSLNHERVDPDYET
                     FGAYYFTNDLDNWTLAPSLRLWKGKLTLALNAGVQRNNLDATKINTMHRIVSSCAISF
                     VPSASWMMSFSFSDFTSYTNRRPLTDPFWSPSPADTLNFYQVSQQGNACVMYMFGKKE
                     MKQNFSLSGSYQRSMQQGGAMGTDIYNGNVSYSLQLAKQKMSFSVLGNFNEAGTGAVV
                     SEYFGPGWQGSCSLLKNTLRCSAGTVYNVSLTNGAAAKICSYRAQLSYSPGMKNKKYG
                     RPVLSLNAVYVQRFASQDTGELTVTVNAGYSF"
     gene            complement(9183..12599)
                     /locus_tag="VFU15_04895"
     CDS             complement(9183..12599)
                     /locus_tag="VFU15_04895"
                     /inference="COORDINATES: protein motif:HMM:NF039936.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEU4717144.1"
                     /translation="MRPLHICFRLLLCVFFFTGNFSRAQTLSAISYPQISCSGTCPSQ
                     VTLLTYVSAAYFDSTNEVIYLGGKFNDAGGNTRNGLAAVDAVTGSLLPLNVNVGGGEV
                     LSIAKSGDTLFIGGTFSSVNSMARKRIAALSAATGNLLPAFSTGTSSANDSVNALLVL
                     GGKLYAGGKFSSIENNARTNIACFSVGGAIQAWMPSPAFPANEPVRKFGWYSGNIVVL
                     KDDISIDGSDLVAVNIFSGNANVRVYTDVTQTISDFAMRGSVAYFAGPFQTVNGYSLN
                     YAAACDLSNGNLAGWNPSIAVGLFDTRSRFSIAYYRDSLYIGVMDASSQQPQFHRLYV
                     SYYNSAAPSNIRVLKTYSSNLPGLNGYYHDHLLIANARMIEIERYAQHTSFPDGTLSC
                     EIYSWCLKPPPTPGAFVIHPLSACPGDTNVMYSIVPQSYFSSYVWTSNTPDIVPSGNT
                     ANASVDFSETFSGGAIKVYGVTSCGTSTPAFRTATVAALPVPDANAGNDDTVSCFPVQ
                     HYLHGSSLTPGASFEWNGPSGNFMTDSLPVTVPGTYVLTIQGPNGCRKRDTALVIADT
                     LPPVLVPFGSIPPLTCRDTTVLLDAASLYGADSLYWSGPGLSAHNDPALVSQMGNFLL
                     TVSGRRNGCASHDTLFVAQDLAPPPANVVAQDSLLTCRRDSVLLDAFSADANAVFQWS
                     DSSGNYFSNPYNATHFGIYQLHATDTVSGCTNSSQMFFVDEWTTPPGIDPLPDSVFLD
                     CSYSSLTLHAFSQTAGASFSWNGPSSFSATDSAVVTQQGNYSVTAENPLNGCTTVDSV
                     YAGFAMTLDLSVSSDTVICPGSGAVLQVFPIGGTAPFSVSWNNNAGNSSPATVYPADT
                     TLYVVAVTDHSGCTGTGSVVVNVPDPIDGNVVSFQPCDPSHATGQLQVHCVNGVPPYA
                     FSIDNGLSWNSSGVFGNLAYGTYILLAADAIGCTWNAAATIDTASLRPAPEFLVSTSP
                     QAGDTIVIVDISNPRPDSVRWDFPPGTVVTDSSMFAPAIIPGDTGNFSITMHAFFGSC
                     EEIYSRLVRIEPFDSLQATPWNDNGIDSLVIYPNPNNGVFTATVLLGAKQDFILLVYD
                     ANGTEMTRAQVHDSDEWTGQISVPGAVPGNYVLRVIAEYDARQLTFVIAQ"
     gene            complement(12718..12930)
                     /locus_tag="VFU15_04900"
     CDS             complement(12718..12930)
                     /locus_tag="VFU15_04900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717145.1"
                     /translation="MCKTPLLIICFLLPFFSVAQDSLAFCRFLSRFPLMKTPACIAFS
                     KEEQEELDRGWPDSMSFVHLNHLMEK"
     gene            13060..13503
                     /locus_tag="VFU15_04905"
     CDS             13060..13503
                     /locus_tag="VFU15_04905"
                     /inference="COORDINATES: protein motif:HMM:NF022182.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HEU4717146.1"
                     /translation="MEPIQNYYSAVEEVIQSFGVDPATCRGQQIGQWNLKLGSASVWV
                     DVWQSKDKDGNFIDGGYLQVMAPVVDVPPGNAEFTRELLEINHRLYGVAFTIHGNSAY
                     IKAIRELEGLDKSEISATLNRVGIYADDYDDQLKAKYWPVSGGRG"
     gene            complement(13587..>14228)
                     /locus_tag="VFU15_04910"
     CDS             complement(13587..>14228)
                     /locus_tag="VFU15_04910"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEU4717147.1"
                     /translation="HEFRTPLGTLLSSLSILEKYVKPGSGSAEEKHINRIRSSVSHLV
                     EILDDFLSLDKLENGKAEIQPVPFNVESFIGSVVEEMQTLLKEGQKIEWNYKGEAEFR
                     LDTRVLKNILYNLLSNAIKFSGKGSGITIHASATEHALMIRVKDKGIGIPEADQPFMF
                     MRLFRAKNAINIGGTGLGLNIVKRYIELMNGEIGFISEEGKGAEFIVQLPLDK"
BASE COUNT         3510 a         3923 c         3701 g         3094 t
ORIGIN      
        1 agaaccactg tccgctcatt tcatccactt tggggctgga cagcgcaagc ggcttcccgt
       61 ccttcccgta aaacaccgcc ttcaccgcca ggaaatcgtt cacttccgca gtgatcattt
      121 tttttccgcc cgggtaaaaa accgtgtacg ttccgttcgc cgtaccggaa acataatcca
      181 gggaactccg cagtttcccg ccgggataaa atgttttcca tgttccgtct ttccgggtac
      241 cgctgaggtc gccttcttct ttgatgtacc ccgtcgtgtc gaaaaactgt tcgtgcgttc
      301 ttgccaaaaa cgtttccgac aggtaaccgg taaattcttc cggtcgcagc gcagaacgtg
      361 aaatgcagct gagcctgcca ttcccgtcga atgtgtaaac cgaatcgggc ggaggttgcg
      421 gcagcgtgtc gtattcgcgc gttgaatttc ccgccgggag aaaacatccg ttgtggaacc
      481 aggtggtttg cagcagcaca ccggaagggg aataatattt ccacgcaccg gtttttattc
      541 cgtcccggaa ctgtccgctg aattcgattt ttccgccggg ataaaaaagc ctgaattccc
      601 cgtcatacaa tccgctcttc cagttgcacg tcatcgaata tccgccggaa agccgcgccc
      661 gttctccgcc gtcctttttt ccgccggaat aatgcgaata aaactgcggc agggaatctt
      721 tccacccgct gtcgggatta tcaataaccc atccttcgtg ctttgtaccg ttcagcgtac
      781 ttgcagaacg tacgcggccg ttgggccaga agcattgcgt ggtgacaaac ccgtctgccc
      841 ggtaactctt cgccatgagc agctttccgt ctgcgctcca ggatgaatca ctcatcaaag
      901 tatcgagatg ataatggctg atccaaataa cagctccttt gctgttgtaa cttatccatg
      961 cgctgtcttt tctgcccatc cggtaaaacg atttcgtgac cagtaaactt ttcccggcga
     1021 cgagatcttc attgtaataa tcctgttcgc cgtcataata gcctttcgaa taacgatgca
     1081 cctgcagcac atttccattg gctgcggaaa ccgttttcca ccggccttcc gccatgtgct
     1141 ttttcacatg tcctttggtg accgcatacc cggttcctga aacgatgcgt ccggattttt
     1201 taccgtacct cagcgggttc ccggcttcct gtgcgtgagc gggaaggaga aaaacaagcg
     1261 gcagaaaaaa ggcaagcagt gttttcaccg gggttgcaga tgaaagtaag aaacctgttc
     1321 ctaccgtaag acgcattttc ccgggttact gcataaaaaa accggtcgca tttgcatgac
     1381 aaccgggtag aagggtcctg gcaatggttt acttaacgag cacctgcctg ctgtagaact
     1441 cctggcggga gcagccgaca atggtgtacg ttccgctgga aagcgcggga tcgagatcgg
     1501 cgcattccat gggatttccc ttgccggtaa tatcgaccat ggaatagaat tcttcgcctg
     1561 ccgcgttctg cacgatgatc agcacggagc tgtcggaagg agctgtgttg taggaattgg
     1621 tggcggccac gggtgaaacg ggctccatgt tatcgtcata ttttacggga accgtattgg
     1681 aatacgtgac cgatccgtcc atgttcgtca tcctgagacg gtaccaggaa agacctttgg
     1741 ccggcgcgaa atccgtttcc atgaattcca tgcctgcaag atctccggcc tgcactgttc
     1801 cgaaaatatc ataatcgctt ccgttcttcg atttctgaag atcatagctg atcacatcct
     1861 tgctttccgc ctgccaggaa agaagaacat gtgaaccgtg ggcgatggcc gagaaggaag
     1921 tgaacgttat ctcgtggccg ggctcctggg ctttcgcagc aaatgaaaaa acgccggcca
     1981 tgacgagagt gagaagtaaa cgcattttta tttgtgtgtt aaggattttt gagcttaaca
     2041 aatttaaaaa aacgggattc atgtgcaagt gaatgggaaa aaacagttat gaagactttt
     2101 cgacacttca tacgttccgg taacgcttct gaaggtctgg cacgacgtgc ttttcaatag
     2161 ctttgcacaa tgagaatgaa gcttccgttt ttcgcattta ccgccctttt ccctttcctc
     2221 cttgccgcac aggataaaag cggcctgcac gtcaccgata cgttccacat caggagcgac
     2281 ggaggatggg attacatcgc cgtcgaccac gacctgaacc ggctgtatgt ttcgcacgga
     2341 acgcaggtga atattctcaa tgaaaccacc ggcgattccg tcggcatcat cccgaacacg
     2401 acaggcgtgc atggcattgc ttttgcgccg gccttcggca aagggtacac gagcaacggg
     2461 aagctgggca cagtcaccgt gttcgacctg aaaacgaaca gcgtgctgaa gcaggtacac
     2521 gtgggggaaa acccggatgc gatcatgttc gatgattatt cgaaaagaat ttacacatgc
     2581 aacgggcaca gccacgacat gagcatcatc gatccgcaga aagacacgct ggtgggcacg
     2641 atcccgctcg gcggaaaacc cgaaacggct gtttccgacg gtaacgggaa acttttcgtg
     2701 aacatcgaag acaaaagcga gattgccgtg atcgacctgg cgaaagactc cgtcatcgcg
     2761 cgatggaaaa cgggcagcgg ggaagaacct tcgggactcg ccatcgaccg gaagaacggc
     2821 attctcttca gcggctgcag caacaagctc atgatcgtga tggactgcaa aaccgggaac
     2881 gtgatcgcag agcttcccat cggcgacggc tgcgacggca ccgcgttcga cccgggaacg
     2941 atgaatgcgt tcagctcgaa cggggacgga acgctcaccg tcatccacga ggacagcccg
     3001 aaaaaattca cggtcaccgg caacgtagcc acgaaacgcg gcgcacggac tttgtgcgtc
     3061 gacctgcaga cgcataagat ttatcttccc acggcgcagc tcggcgatac gccgccggca
     3121 acagcagaca acccgcgccc tcgcccgaaa aaaattcccg gcacgttcca ggtcatcgtg
     3181 gtcgggaaat aattccggtt gccgcataaa agatcttcac cttccgaaaa atgaaaaaca
     3241 gaattttcac tgccgcgctc ttcctcctgc tttcgctttc gctcattgcg caaaaacccg
     3301 ttgcgcgcat cgcgttctgg aacgtggaaa atcttttcga cacgatcaac gacccgaaca
     3361 tcaacgatga agagttcctt ccgtcggcga aagatcactg gacatcggac cgttacctgg
     3421 cgaagatcaa tcacctcgca cagggaatcc tcgccatcgg cgacggcgca ggcccggaca
     3481 ttctcggcat ggccgaagtg gaaaatgaag gcgtgctgaa agaccttacg cagaaaaccg
     3541 acctgaaaaa acagaagtac ggcatcgtgc attacgattc accggacaag cgcggcatcg
     3601 acgtggcgat gctgtataaa aaaagcaaat tcaaagtgct cgactcgcgc ccggtgaacg
     3661 tgtctgttcc cgccgacaca tttttcacgc gcgatatcct cgtcgtgaaa ggcatcctga
     3721 acaaaaccga cacggtgtgg atcatcgtca accactggcc ttcacggcgc ggcggcgcgg
     3781 aagccagcga atcgaaacgc atgatcgcgt cgcggaaatt gcagggcatt ctcgacagca
     3841 tcatgaaaat gaacccgcgt tcgctggtgc tcgccatggg tgacttcaac gacgagccca
     3901 cgaacaacag catcatgaac ctcaaatgca acgactggct ggacccggcc atgcagcaaa
     3961 agctggacac gctgaacaag atcatcggcg agagcatcga caaccagggc gccgggtgga
     4021 tgatggcgcc atcgcagaat tgttttcatg atctcatgga ttcactgaaa gacgccggcg
     4081 acggatcgta tcattaccgg aaagaaaaag acatgatcga ccagttcctt gtcaacggga
     4141 acctgtacgg aacgggctac cgcatcacgg tccattccgc gcacattttc aaagcggatt
     4201 ttctcaccgg cgaaaattac aagagcgatc ctccgggtcc gctgcacact tacgcaggtt
     4261 cgcgttacat cggcggctac agcgatcatt atcccgtgta cgcggatgtg gtgacgacag
     4321 ggaaaaagtg attcccggcc cgaaagttga aaagccggaa tgttggaaag caaaaggcaa
     4381 acaaccagcc tgcaacttca aaacgttcca actttacaaa ccgtcatccg catagcatcc
     4441 cccttcgtac ttttgccccg catgaacagt gatccgaaag aagagcttcg ccgtttcgtc
     4501 gagcttcttt ccaccgaaaa agacgaagat taccgccagt acatcgcgca gttcgaacgc
     4561 tgcagcataa ccgaacgccg gaagaacggc gtgacgtggt atcctgtgcg catcctttcc
     4621 gaagaaatgg gcgccggcga ttacatcagc atcgaagtcg agcgcacgca gggcatgaaa
     4681 gagctgcacc agttcggcgg gggaaagatg gtggagatct tcagcaatac cggcgacaac
     4741 ggcagcgaaa cgtggaagat cagcgggacg atcaggaatg tgtggggcga ccgcatgcgc
     4801 atttcgttca tgaccgatga gctgcccggc tggaccgaac gcggcaagct gggactcaat
     4861 cttcttttcg acgagaacag ctaccgcgaa atgcttatcg ccctgcagaa agtgatcgat
     4921 gcgggcgatg accgtgaaag ccgccgtctc gcaaaactgc gcgatgtttt ttacggcctg
     4981 gaaaaagcgg agttcgaaaa agaagacacg aacctcgtgc tgcccgggtt gaataaatcg
     5041 cagaatgaag ccgtgcgtca catcatcgcc gcgaaagaag ttgcgctcgt gcatggtccg
     5101 ccgggaacgg gaaaaaccac gacgttcatc caggccattc gccatacgct catgcatgaa
     5161 aagcaggtgc tggtgtgcag cccgagcaac atcgccgtcg acctgcttac ggaaaaactc
     5221 gtgcagcagg gcatcaacgt gctccgcctc ggcaatcctg cccgcgtttc ggaagaagtg
     5281 ctgaacaaca cgcttgacgc gaaaatgatc gcgcacccga attacaaaga gctgaaagaa
     5341 tacaggaaac gttcggaaga atttttcgcg atggcgaaaa aatacaagcg caatttcggt
     5401 cacgccgaac gcgagcagcg gaacctgctt tacaacgagg cgcggcagct cgtgaaagaa
     5461 gcgacagcgc tcgaagatta cattttctgg gagcagttcg ggaacgcgca ggtgatcgcg
     5521 tgcacgccgg tcgtttccgc cgggaaaatg ctgcgcgaca aaaaattccg cacggttttc
     5581 attgacgaag caggacaggc gctcgagccc atgagctgga tcccgatcac gaaagcggaa
     5641 cgcgtcatct ttgcgggcga ccacttacag ctgccgccga cggtgaaatc gaaaaaagcg
     5701 gaagacggcg gactcaaatt cacgctcttc gaacgcctca tgcgcacaca gccgcacgcg
     5761 tcggtgatcc tgcgcacgca ataccgcatg aacgaaaaga tcatgcagtt ctccaaccgc
     5821 atgttttacg gcaacaagct cgaagcggat gtttccgtga aagacaacgt gctcagcttc
     5881 gacggggaag acgacctgct caatcgtccc gtggatttca tcgacacggc cggctgcggt
     5941 ttcagcgaag agcagaaccc cgaatcgctg agctattcca acccggaaga agcgaacctg
     6001 ctgctgcgtt acctcgaacg cgtgctggac cagtatgaaa aaatgccgga cgattccctg
     6061 cgcatcggga tcatcgcgcc gtaccggcag cagaccgaat ggctggcgca gcagcttgaa
     6121 gaacgggaat ggaaaaagaa ttcacgccac gctttttccg tacgcaccgt cgacggtttc
     6181 cagggacagg aacgcgacat catcgccatc agccttacgc gcagcaacga caacggcgag
     6241 atcggtttcc tcgcggacac gcggcgcatg aacgtggcgc tcacgcgcgc gaagaaaaag
     6301 ctcatcatca tcggcgacag tgcgacactg gcgaatcatt ccttttacaa atcatttctc
     6361 gattacgtgg aagagatcgg cgcatacagg agcgcgtggg agatgatgga gtgaagctgc
     6421 gtccggcgaa tcccggaaaa gcgacaagcg ctcacagaac tgcggccgtc cttcttttcc
     6481 gtaagccagg agaattgcga aggcgcctgc cgggaacgga tttcaacgta tacggccggt
     6541 tgaatgcttc ctgcgacagg ctgaaatatt cagccagcct gcgtatcact tccttcgaca
     6601 accctttttt gtaattcaaa attcctgaaa cataaccctt gcttacaccg agtagcctgg
     6661 caagatcttt cgctttcata ccatgatcac tcatcagcga aaggagaagc tcgacagggg
     6721 tcagctgacg aaaagcgatg ttcctgtgca gctcgtccca tttcccgacg agcaaggtaa
     6781 gcagggcaat ctcatccttc tgctccttcg ttttctttcc ttcagcaaaa acgagctctt
     6841 ccagcattct gcagtagcgg ttgtattgct tcactgacgt aattactttg tatttcagtg
     6901 tttccatttt tttgatcaat aatcggatct tatgtattgc tccccggaat cacacagctt
     6961 gtcgtattct gcatgcgtac caatccagca aacaaacagg tgaacttcac tctcgccgaa
     7021 agcatacttg gcaatcatac ggtaattatt tcctcctata tcgaacacaa tccgggacga
     7081 tccattcccg agaaaatcag ccgaagcgaa cgttttccgt atgtcctccg gtttttccca
     7141 atctgcaagg aatacctttg aaagccatgc gttgaacgaa gatctccctc tcgcctgctc
     7201 ctcgatatac ctccggattg tctgctcttt taccaggtga actttcatac ataaatacgg
     7261 ttaatttttc aaaaagttca cctttggtaa actttttcgg gctaaataag gttaaacagt
     7321 gcaggcatct tcccggactc cgtcctcctt tcaaaacgaa taccctgcat tcaccgtcac
     7381 tgtcagctca ccggtatcct gcgaggcgaa ccgctgtacg taaactgcat tcaggctgag
     7441 caccggccgg ccgtattttt tgtttttcat tcccggcgaa taactcagct gcgcgcggta
     7501 cgagcagatc tttgctgccg cgccattcgt cagcgagaca ttatataccg tcccggccga
     7561 gcagcggagc gtatttttca gcagcgaaca gctcccctgc catccgggtc cgaagtattc
     7621 actgaccacc gcgcccgttc ccgcctcgtt gaaattccca agcacggaaa acgacatttt
     7681 ctgttttgcc agctgcaggc tgtaagaaac attgccattg taaatatccg ttcccatcgc
     7741 acctccctgc tgcatggaac gctggtacga accgctcagt gaaaaattct gcttcatctc
     7801 tttcttcccg aacatgtaca tcacgcacgc gtttccctgc tgcgaaacct ggtaaaagtt
     7861 cagcgtatct gccggtgacg gcgaccagaa cggatccgtc agcggacggc ggttcgtgta
     7921 gctggtaaaa tccgaaaagg aaaagctcat catccatgac gcggaaggga cgaacgagat
     7981 cgcacaggaa gaaacgatcc tgtgcatggt attgatcttc gtcgcgtcaa gattattgcg
     8041 ctgcacaccc gcattcagcg cgagcgtgag ctttcctttc cacaggcgca acgacggtgc
     8101 cagcgtccag ttgtcgaggt cgttcgtgaa ataatacgca ccgaacgttt cgtaatccgg
     8161 gtcgacgcgt tcgtgattga gactgagcgc aaagatcttc ccggtgtaac ccgccgacat
     8221 tttccacgca tggaaaaaag ctgtcgtggt gtgcgtcgtc atcatccatt tttcgagacc
     8281 cgaaaaaccg gtcgtcacaa ccggggacaa gctgttcttc gttatgcccg acaaagccat
     8341 ttcgctttcc acgtgaaata tcctgaacag cgtgatcttc ccggaaacgc tcatcgccgt
     8401 attttccatt ggagaaattc ccgcttccgg cgggatgaac gcgagcgagg ctgcatcatc
     8461 tttcgctgag aagaatgttg cactcaatgc attcccgttc ttatcgtacc ccatcttcgc
     8521 ggcgcagcct gtccggcgga aagccgcatt ttcaaaagaa accgccgcat ctccttcatc
     8581 cgctttgctg aatcttccgt acatcgcacc aacgtaccag ttagcgggtt tcagctccat
     8641 tcccgcgccg gtaaaaattc ttcccgacag cgtgtacccg gaaaaattca tcgcggttgt
     8701 cccgatatgc gctgtcgccc atttgtaatg cggatggcag ctctgcatgt tgaacggctg
     8761 cgtatatgtt ccgtggttgt tcgtgtagct gtacgtaaac ggcaggtcga caccgagaaa
     8821 gttcatgtcg atgttcccgt tgaaatacca ggtgaacggg tcacggcggt tttgcatacc
     8881 ctgtgcatcg tagaaaaccg cgttataatt cattcccccg ctgacggtga gcatgtcttt
     8941 cttcccgatc ctgccgaggt cctgggcctg cacattttca ttggtgaaaa aacaaagaag
     9001 gagaagaaaa gcataaccgc gaaggcacaa aaagatctcc ctccgtccgt acgcggatcg
     9061 ggaaccgttt tcagctccgc gtttccggtt tacaaatcct gctgcgctcc cgtgaaacaa
     9121 ttcccgtgtc aaaaaaattc cgcgcaaaga cgcgaagaaa aaaatgcggc gaactgaatt
     9181 tttcattgcg cgatcacgaa tgtcagctgc ctcgcatcat attccgcgat cacgcgcagc
     9241 acgtaatttc cggggaccgc gccgggcacg gagatctgtc ccgtccattc atccgaatca
     9301 tgcacctgcg cacgcgtcat ctccgttcca ttcgcatcgt aaacgagaag aataaaatcc
     9361 tgtttcgcgc cgagcaaaac cgttgctgta aaaactccgt tgttaggatt gggataaatg
     9421 acaagggaat cgatgccgtt gtcattccac ggcgttgcct gaagactgtc aaacggttct
     9481 atgcggacca ggcgcgaata aatttcctcg cagctcccga aaaacgcgtg catcgttatg
     9541 gaaaaatttc cggtgtcgcc cggaatgatg gccggcgcga acatggatga atccgtaaca
     9601 acggtgcccg gcgggaaatc ccatcgcact gaatcgggtc gcgggttgct gatgtcgacg
     9661 atgacgatcg tgtcgcccgc ctgcggggat gtgctgacga gaaattccgg agccgggcgg
     9721 agactggctg tgtcgatcgt cgccgctgca ttccacgtac agccgatcgc atctgccgcg
     9781 agcaaaatgt aagttccgta cgcaagattt ccgaaaacgc cgctgctgtt ccagctcagc
     9841 ccgttgtcga tggaaaatgc gtacggcgga acaccgttca cacaatgcac ctgcagttgt
     9901 cctgtggcgt ggctgggatc gcagggctga aatgaaacga cgttcccgtc aatgggatcc
     9961 ggcacattca cgacaacaga acctgttccc gtgcaaccgg aatggtccgt tacggccact
    10021 acgtagagcg tcgtgtctgc aggataaact gtcgccggtg aagaatttcc ggcattgtta
    10081 ttccacgaaa cggaaaatgg ggccgttccg ccaatgggaa aaacctgcag caccgctccc
    10141 gacccgggac agataaccgt atcgctgctc accgaaagat cgagcgtcat cgcaaaaccg
    10201 gcgtacacgg aatcgacggt cgtgcaaccg ttcaggggat tttctgctgt aacagaataa
    10261 tttccctgct gcgtgacgac tgctgaatcc gttgcagaaa aagaagacgg cccgttccag
    10321 gaaaaagaag ctcccgcggt ttgggaaaat gcatgcagcg tcagtgatga ataggaacaa
    10381 tccaggaaaa cggaatccgg aagtggatca atgcccggcg gagttgtcca ttcatccacg
    10441 aaaaacattt gcgagctgtt cgtgcagccg cttaccgtat ccgttgcgtg cagctggtaa
    10501 atcccgaaat gtgtcgcgtt gtacgggttg ctgaaataat tcccgctgct gtcgctccat
    10561 tggaaaacgg catttgcatc ggcggaaaac gcatccagca gcactgaatc gcgccggcag
    10621 gtgagcaggg aatcctgggc aacgacattt gccggaggcg gcgcaaggtc ctgtgcgacg
    10681 aaaagcgtgt catgcgaagc acatccgttg cggcgacccg aaacggtcag caggaaattt
    10741 cccatttgtg aaaccagcgc cggatcgtta tgcgcggaaa gcccgggacc cgaccaatac
    10801 agtgaatccg cgccatacaa cgaagcggcg tcaagcagca ccgttgtgtc acggcacgtg
    10861 agcggaggaa tgctcccgaa tggaaccagc acaggcggaa gcgtgtctgc aatcaccaaa
    10921 gcggtatcgc gtttccggca gccattgggt ccctggatag tgagcacgta cgtccccgga
    10981 acagttaccg gcagcgaatc cgtcataaaa ttccccgaag gtccgttcca ttcgaaagaa
    11041 gcgccgggcg tgagcgagct tccgtgcagg taatgctgga cgggaaaaca gctgaccgta
    11101 tcgtcatttc ctgcgtttgc atccgggaca ggaagcgctg ctaccgtcgc cgtgcggaaa
    11161 gcgggtgtag acgttccgca gcttgtgacg ccgtatactt tgatggcgcc gccgctgaac
    11221 gtttctgaaa aatccaccga agcatttgcc gtattcccgg aaggaacaat gtccggcgtg
    11281 ttgcttgtcc acacatagct gctgaaatac gattgcggca cgatggaata catgacgttc
    11341 gtatcgcccg ggcaagcgct caacggatga ataacgaacg caccgggcgt gggcggcggc
    11401 ttcaggcacc aggaatagat ctcgcagctg agcgttccat cgggaaacga cgtgtgctgc
    11461 gcgtaccgct cgatctcgat catgcgcgca ttggcgatga ggagatgatc gtggtaatag
    11521 ccgttgagtc ccggaagatt cgacgaatat gttttcagca cgcggatgtt cgacggcgct
    11581 gcgctgttgt aatagctcac gtaaagccgg tggaactgcg gctgctgtga agacgcatcc
    11641 atcacgccga tgtacaggct gtcgcggtag tacgcaatgg aaaaacgcga acgcgtgtcg
    11701 aacaaaccca cagcgatgga agggttccac ccggccaggt ttccattcga aagatcgcag
    11761 gcggcggcgt aattcagcga atagccgttc accgtttgaa aaggtccggc gaagtaagcg
    11821 acagagcccc gcatggcgaa atcggaaatg gtctgcgtga catccgtgta cacgcgcaca
    11881 tttgcattgc cggaaaaaat attcacggcg acgaggtcgc ttccgtcaat ggaaatatcg
    11941 tctttcagca ccacgatgtt cccgctgtac cagccgaatt tccgcaccgg ctcattcgcg
    12001 ggaaatgcag gcgacggcat ccacgcctgt atcgcaccgc cgacagaaaa gcaggcaatg
    12061 ttcgtgcgtg cattgttttc gatcgaagag aatttcccgc cggcgtacaa ctttccgccg
    12121 agcacgagca atgcgttcac cgaatcattg gcagaagaag tcccggtgga aaaagcgggc
    12181 aacaggtttc ccgtcgctgc gctgagcgca gcgatgcgct ttcttgccat ggaattcacg
    12241 gacgagaacg tcccgccgat gaaaagcgtg tcgcctgatt ttgcaatgga gagcacttcg
    12301 cctccgccca cgttcacgtt cagcggaagc aggctgccgg ttacggcatc aacagcggcc
    12361 agcccgttgc gcgtattccc gccggcgtcg ttgaatttcc cgccgaggta aatgacttcg
    12421 tttgtcgaat cgaaatacgc tgcggaaaca tacgtcagca atgtcacctg ggaaggacag
    12481 gttccgctgc agctgatctg cgggtaggaa atggcggaaa gcgtttgtgc gcgcgaaaaa
    12541 ttcccggtga agaaaaaaac gcagaggagg aggcgaaagc aaatgtgcag ggggcgcata
    12601 atgaaggacg gcaaaaataa attcccttct atgcgaaacc cgttcttaac tgcttatatc
    12661 cgtttgcaca cggaaaataa taccctcatc acccgcattt cccggtcagt cccgtcttta
    12721 tttttccatc aggtgattaa gatgcacgaa cgacatggaa tccggccacc cgcggtcgag
    12781 ctcctcctgc tcttctttcg aaaaagctat gcaggcgggc gttttcatca gcgggaaacg
    12841 ggaaagaaaa cggcagaacg cgagactgtc ctgcgcaacg gagaaaaacg gcagcaggaa
    12901 acaaatgatc agcagcgggg ttttgcacat gctgtatgaa gttaccgctt tccgactttt
    12961 caactccccc gcttccatga ctttctttta aaactaacac aaccgtccgc ggaagttttt
    13021 gttctatatt tcacgcctaa acacttaact ttttttacca tggaacctat tcagaactat
    13081 tacagcgccg tcgaagaagt gatccaatcg ttcggcgtgg atcccgccac gtgccgcggg
    13141 cagcagatcg ggcagtggaa cctcaagctc ggctccgcga gcgtttgggt ggatgtgtgg
    13201 cagtcgaagg acaaggacgg aaatttcatc gacggcggtt acctgcaggt gatggcgccg
    13261 gtagtggatg ttccgccggg aaacgcggag ttcacgcgcg agctgctcga gatcaatcac
    13321 cgcctctacg gcgtggcgtt caccattcac gggaacagcg cgtacatcaa agcgatccgc
    13381 gagctggaag gcctcgataa atcggagatc tccgcaacgc ttaaccgcgt cggcatttat
    13441 gccgatgatt acgacgacca gctgaaagcg aagtactggc ccgtttcggg cggcaggggc
    13501 tgaagctgaa catctcaaat cccggaccgg cgacttcctg tctcctgccc gggatttatt
    13561 ttttgaagcc gtccggcaac cggcgcttat ttatccagcg gaagctgaac aataaattcc
    13621 gccccttttc cttcttcgct gataaaaccg atctcaccgt tcatcagctc gatatagcgc
    13681 ttcactatat tcaggcccaa tcctgttcca ccgatgttga tcgcattttt ggccctgaaa
    13741 agacgcatga acatgaacgg ctgatcggcc tccgggatac caatcccctt gtctttcaca
    13801 cgtatcatca gcgcatgttc cgtggcggag gcatgtatcg ttatgccgga tcctttaccg
    13861 gaaaatttta tcgcgttcga aagcaggttg tacaggatat ttttcagcac ccgtgtatcg
    13921 agccggaact ccgcttcccc tttatagttc cattcgatct tctggccttc cttcaacagc
    13981 gtctgcattt cctccacaac tgagccgata aaactttcca cgttgaaagg cacgggctgt
    14041 atctccgcct ttccattctc aagtttgtca agcgaaagaa aatcatccag aatttccacg
    14101 agatgactga cagaagaacg gatgcggttg atatgctttt cctccgcact tccactgccc
    14161 ggtttcacat atttttcaag aatggacagg cttgaaagaa gtgttccgag cggcgtgcga
    14221 aactcgtg
//
LOCUS       DASTSF010000169         3755 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_136407,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000169 DASTSF010000000
VERSION     DASTSF010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3755
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136407"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            293..520
                     /locus_tag="VFU15_04915"
     CDS             293..520
                     /locus_tag="VFU15_04915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717148.1"
                     /translation="MEHSRQFPHRTFKHHLFVGGGTLIVQDSDLSQPNSSNLGTWTIK
                     IVAASSSQRKAHPNLDWNNYNAVKKQFDISD"
     gene            complement(715..798)
                     /locus_tag="VFU15_04920"
     tRNA            complement(715..798)
                     /locus_tag="VFU15_04920"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(762..764),aa:Leu,seq:taa)
     gene            complement(915..987)
                     /locus_tag="VFU15_04925"
     tRNA            complement(915..987)
                     /locus_tag="VFU15_04925"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(952..954),aa:Gly,seq:gcc)
     gene            complement(1203..>3755)
                     /gene="mutS"
                     /locus_tag="VFU15_04930"
     CDS             complement(1203..>3755)
                     /gene="mutS"
                     /locus_tag="VFU15_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009860461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEU4717149.1"
                     /translation="AKHPGALLLFRVGDFYETFGEDAVKASAILGIVLTKRANGSASY
                     VELAGFPHHSLDTYLPKLVRAGMRVAICDQLEDPKMTKTIVKRGVTEMVTPGVSFNEK
                     VLDQRSNNFLAALHFEGEDAGVAFLDVSTGEFLVAQGKTAYVDKLLQNFRPSEVLFQK
                     NRKKQLSEEFGDRFYVFGMEDWIFTPEFSTEKLMKHFGTNSLKGFGIEEIPLGVIAAG
                     AALHYLSETQHDRVQHISAISRIEEDKYVWLDRFTMRNLELFGSANENAKTLISVIDR
                     TVSPMGSRLLKRWLALPLKDIDAISDRLEGVQALLEKNEIRDKLSDLISSAGDLERLV
                     AKAAAGRINPRELLQMRRALEIIPPVKELCAQSGNAQLKKTGERLDECAAIRERIKKE
                     LHEEPPVVVSKGGVMAEGVDAELDELRRMAFSGKDYLLQLQQRESERTGIPSLKVAFN
                     NVFGYYLEVTNAHKDKVPGEWIRKQTLVNAERYITPELKTYEEKILGAEEKILSIEQR
                     LFAELVQSLAEWIAPIQSNASLLAHLDCLTGFAVLAQQKNYVRPHVNDSLVIDIKNGR
                     HPVIEQQLPAGESYIANDIYLDSTSQQILMITGPNMSGKSALLRQTALIVLLAQMGSF
                     VPATHAQIGLVDKIFTRVGASDNISSGESTFMVEMNETASILNNISERSLVLLDEIGR
                     GTSTYDGISIAWAIAEFLHEQPSARAKTLFATHYHELNEMAERFPRIRNFNVSVKEVG
                     NKVIFLRKLAPGGSEHSFGIHVAKMAGMPRKVVERAKEVLAQLEKSHSGAELMNEKHV
                     KPKEEDYQLSFFQLDDPVLEGIREEILKTNIDTLTPVEALMKLNEIKKLLGK"
BASE COUNT          807 a         1093 c          928 g          927 t
ORIGIN      
        1 gtttcgtccg gcaggacggg gatcgtttat tctaaagaat attcaaatac aactcctcgc
       61 tgctcctcat caccatctgt agttttcttc catcggtatt gaacattgtc ccattgaatg
      121 agcgattatc tttcgtttcc aacgagcaag ttgccgactg tttgcttccg gtgaattcat
      181 tcagttcgat tttacctcca gcgatagtcc cggtgagcat gtattcaact ccttttcggg
      241 atagataatc gtacgtgccc gtgatctttc catgttcgta gtcgaatgtc agatggaaca
      301 cagcaggcag tttccccaca gaaccttcaa gcatcacctc tttgttggtg gtggaacact
      361 catcgtccag gactccgatc tgagccagcc gaactcaagc aatctcggca cgtggaccat
      421 caagatagtg gcggcatcca gttcgcagcg gaaagcgcat ccgaatctgg attggaacaa
      481 ttacaatgcc gtgaaaaagc agtttgatat cagtgactga tctttagccg ttaacaagaa
      541 agcagaaact gcaggaaacg gtcttcgccc cgggtctttt ttatgctaaa caaaagcatg
      601 aagttcgaac cggatcgatc agcggggttt ccagcttaat cagctcaata tcaaacagca
      661 gaatttcaaa gaaaaacggg acttctggtt tttcaaccga aaagtcccgc tctgtacccg
      721 gggcgggact tgaacccgca cggtcttgcg accaagggat tttaagtccc tcgtgtatac
      781 cgatttcacc acccgggcat tgtacctttt ccctcacgct gtcccttccc ttcttttgcc
      841 gggctgagta gcctgcgaag aaggcgtatc gaagcccggc aaaaaaaaac ctcccttttc
      901 agagaggttt tcagagcgga agaccggtct cgaaccggcg accttaacct tggcaaggtt
      961 acgctctacc aactgagcta cttccgctat tatttattcc atcatttaaa gaaccggcga
     1021 tcttaatccg ccacggcgga ttacgctttg tcaactgagc tgcttccgcc attgcccgga
     1081 ggcacgaact aaagaacggt gatcaaagtt cccaaaatgg gagtacaaaa gtagtatcct
     1141 cccccgaatc tgcaaaattt tgatgctttt acctgaaacc tactggtgga aagggtttca
     1201 gcctatttcc ccagcagctt tttgatctcg ttcagcttca tcagggcttc caccggcgtc
     1261 agcgtgtcga tgttcgtttt gaggatttcc tcacggatgc cctccaggac cggatcatcg
     1321 agctggaaaa agctgagctg gtaatcttcc tctttcggct tcacgtgttt ctcgttcatc
     1381 agctcggcgc cgctgtgcga tttttcgagc tgggcaagga cttccttcgc ccgctccact
     1441 actttcctcg gcatgcccgc cattttcgcc acgtgaatac cgaagctgtg ctcacttccg
     1501 cccggcgcca gcttgcgcag gaagatcact ttgttgccca cctctttcac gctcacgttg
     1561 aaattgcgga tgcgcgggaa acgctcggcc atctcgttca gttcgtggta atgcgtcgcg
     1621 aacaacgttt tcgctcttgc agacggctgc tcgtgcagaa attccgcaat ggcccacgcg
     1681 atggaaatac cgtcgtacgt gctcgtgccc cggccgattt cgtcgagcag cacaaggctg
     1741 cgctccgata tgttattgag gatgctcgcc gtttcgttca tctccaccat gaacgtcgat
     1801 tcacccgaag aaatattatc gctcgcgcct acacgtgtga aaattttatc aaccagcccg
     1861 atctgcgcat gcgtcgcagg aacgaacgat cccatctgcg ccagcagcac gatgagcgct
     1921 gtctgccgga gcagggccga tttacccgac atgttcggtc ccgtgatcat cagtatctgc
     1981 tgcgaggtgc tgtcgaggta aatgtcgttc gcgatgtagc tttcaccggc aggaagctgc
     2041 tgctcgatca ccgggtgacg gccgttcttg atgtcgatca ccagcgaatc gttcacgtgc
     2101 gggcggacgt aattcttctg ctgcgccagc accgcaaaac cggtgagaca atcgaggtgc
     2161 gccagcaggg aagcgttcga ctggatcggg gcgatccatt cagcgagcga ctggaccagc
     2221 tctgcaaata agcgctgctc gatgctgaga attttttcct ccgcgccgag aattttttcc
     2281 tcgtacgttt tcagctccgg cgtgatgtag cgttccgcgt tcaccagcgt ctgcttccga
     2341 atccactctc ccgggacttt atccttgtgc gcgttcgtca cttcgaggta atagccgaac
     2401 acattgttga atgccacctt cagcgaagga atccccgtcc gctcactttc ccgctgctgc
     2461 agctggagca ggtaatcttt tcctgaaaag gccatgcgcc gcagttcatc cagttccgca
     2521 tccacacctt cggccatgac gccgcctttg ctcaccacca ccggcggctc ttcgtgcagc
     2581 tcttttttta tgcgttcacg gatcgccgcg cattcgtcca ggcgctcacc ggttttcttc
     2641 agttgcgcat ttcccgattg cgcacacaat tctttcaccg gcggaatgat ctccagcgcg
     2701 cggcgcatct gcagcagctc gcgcggattt attcttccgg ccgccgcttt ggcgacaaga
     2761 cgctcgagat cgccggcgct gctgatgagg tcggaaagct tgtcccgtat ttcatttttt
     2821 tccagcagcg cctgcacacc ttcgagccgg tcgctgatcg catcgatatc tttcagcggc
     2881 agcgccagcc agcgtttcag caggcgcgat cccatcgggg aaaccgtccg gtcgatcacg
     2941 ctgatcagcg tcttcgcatt ttcattcgca gaaccgaaca actcgaggtt gcgcatagtg
     3001 aaacggtcga gccacacgta tttgtcttcc tcgatgcgcg agatggccga aatgtgctgc
     3061 acgcggtcgt gctgtgtttc ggagagataa tgcagcgcag cgcccgcagc gataacgccc
     3121 agcgggatct cttcgatgcc aaaacctttc agcgaattcg ttccgaaatg cttcatcagc
     3181 ttttccgtgg aaaattccgg tgtgaagatc cagtcttcca tcccgaacac gtaaaacctg
     3241 tcgccgaact cttcagacaa ctgcttcttc ctgttcttct ggaaaagcac ttcgctcggg
     3301 cggaaattct gcagcagctt atccacgtac gcggtctttc cctgcgccac gagaaattca
     3361 ccggtggaaa catcgaggaa cgcaacaccc gcatcctctc cttcgaaatg cagtgccgca
     3421 agaaaattat tcgatcgctg gtcgagcact ttttcattga aggaaacacc gggcgtcacc
     3481 atctctgtta cgccgcgctt cacgatcgtc ttcgtcatct tcgggtcttc gagctgatcg
     3541 cagatcgcca cgcgcatgcc cgcacggacc agcttcggaa gataggtgtc gagggaatga
     3601 tgcgggaaac cggcgagctc tacgtacgaa gcggaaccgt tcgcgcgttt ggtgagcacg
     3661 atgccgagga tggcggaagc tttcacggca tcctccccga aggtttcgta aaaatcaccc
     3721 acccggaaaa gcagcagcgc accgggatgc ttcgc
//
LOCUS       DASTSF010000170         4209 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1615291,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000170 DASTSF010000000
VERSION     DASTSF010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4209
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1615291"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..232)
                     /locus_tag="VFU15_04935"
     CDS             complement(<1..232)
                     /locus_tag="VFU15_04935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717150.1"
                     /translation="MKAKLIAWGLILTGSVVLIYAGTCFYAGVSMCLGRTYTKKDLIA
                     NYQLKSKEINEVKTYINSIVPAGKKVFIEFEGN"
     gene            complement(244..2742)
                     /locus_tag="VFU15_04940"
     CDS             complement(244..2742)
                     /locus_tag="VFU15_04940"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU4717151.1"
                     /translation="MKILALNVLCLLSCISFLHAQSATVCGTVKDSTGKPLADVQAGY
                     IGSSKLSMTDANGNFCFNVPAAKEVTIVLRAVGYTGISKKISVSAGDTLKLNFTMKSN
                     YVGNVINVFGDKDDFGRPLPPHIADHLPAPNGPDISNVLKYTAGAFSPNELSTQYSVR
                     GGNFDENLVYVNDIEVYRPFLTRAGQQEGLSFVNIDMTDNVYFSAGGFDAKYGDKMSS
                     VLDVTYRTPTKFRGTASAGLLGFTAEVEGSSTNHRFSWMLGVRNKTNRYVLRSLDTKG
                     DYFTNFIDGQVYLSYAITDEWSLNYLGNYAYNKYNLIPSTRETDFGTINNALRFTVYF
                     DGQEINTFTTNFHALSLDYSDHHHWRSKFILSSFNSYETETFDVQGQYYIDQLEADFG
                     KPTFGQVAFNRGVGTFINHARNYLDAWVNNAEVKAWRYSYSDKYTWNFGLRAQNERIN
                     DQLSEWNYIDSAGYSIPYYPYETIDLQNVVKSTAQLNSMRYSGFVQLKYSTRLRDTSD
                     FSVTAGVRAQYWDLNGQLIVSPRIRLSWKPNWKKDIAFKFATGLYDQPPFYRELRDFT
                     GHVNRDVKAQTSVHFIAGSDWTFQWWGRPFRFITEAYYKYLDNLIPYEVDDVRIRYYG
                     ANLSYGYTYGLDLRLNGEFVEGVESWVNISLLRTRENLYNDYYYIYLNSDGDTIIPGY
                     TYNNIPTDSIKQIPGFIPRPTDQLLMFSLMFQDHLPKVPSCKMNLGLIFGSGLPFGPP
                     THEKYKQVFRMPPYRRVDIGFSYELIKANKPLPVTNPFHVLKSCWAGLEVYNLLQVQN
                     TISYYWVKDVTGRSYAIPNYLTNRQLSVRTIITF"
     gene            complement(2789..3448)
                     /locus_tag="VFU15_04945"
     CDS             complement(2789..3448)
                     /locus_tag="VFU15_04945"
                     /inference="COORDINATES: protein motif:HMM:NF025092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEU4717152.1"
                     /translation="MRKGFFHCLTACAALLCLLPFSSAAQQTIPTDTTRKIIQFSGIV
                     LDADSLNGIPYVAIVVRHSERGVYSNLNGYYSMVVQAKDTIDFYALGYKRASFILPDT
                     ITSDNYNHVQALKLDTILLHEAVVYPWPSKDQFKNAFLTANVPSDDLDRARKNLAQAE
                     MVQLAQNVAMDGGMVYQGQMQQRVNQNYYAGQKPPNNLLNPVAWSRFIQALNNGELKR
                     Q"
     gene            3582..4052
                     /locus_tag="VFU15_04950"
     CDS             3582..4052
                     /locus_tag="VFU15_04950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717153.1"
                     /translation="MRFSEKLLKFTRITSGLLLIFLLSVPALVGGFALISVPDGSTLQ
                     MKTELLRHSPFSDFFIPGVVLFSVNGILAMIAGVLCISGKWIYPRLTVFMGFFVVMWI
                     SIQLFFIHEFIIFQPLYLALGAVLIVLGLLMDRMKNKVSAAGKREIQSAPRPVS"
     gene            complement(4002..>4209)
                     /locus_tag="VFU15_04955"
     CDS             complement(4002..>4209)
                     /locus_tag="VFU15_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010420289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5683 domain-containing protein"
                     /protein_id="HEU4717154.1"
                     /translation="LKNYYERNRDLCFIGMAALYTLNIIDAAVDAHLMTFDVSDNLSM
                     HVQPYYFPSGNRAWCGLNLTLTCR"
BASE COUNT          992 a          992 c         1154 g         1071 t
ORIGIN      
        1 ggtttccttc aaattctata aaaacctttt ttcccgccgg aacaatggaa ttgatgtagg
       61 ttttcacttc attgatctcc ttcgatttca gctggtaatt cgctatgaga tctttttttg
      121 tatacgtgcg cccgaggcac atggaaactc cggcataaaa acaggttccc gcgtagatca
      181 aaaccacaga tcccgtaaga atcaatcccc aggcaattag tttggctttc acggaacaaa
      241 gaattaaaac gtaatgatcg tcctcacgct cagctgccgg ttcgtgaggt aattgggaat
      301 ggcatacgaa cgtcccgtca catctttcac ccagtaataa gagatcgtat tctgcacctg
      361 cagcaggttg taaacttcca gcccggccca gcaggatttc agcacatgga aaggattcgt
      421 gacgggcagc ggcttgttgg ctttgatcag ctcgtaggag aaaccgatat cgacgcggcg
      481 gtaaggcggc atgcggaata cctgcttgta tttttcgtgc gtcggcggac caaacggaag
      541 cccgcttccg aagatgaggc cgaggttcat tttgcaggaa ggaactttcg gcaggtgatc
      601 ctggaacatc agcgagaaca tgagcagctg atcggtgggg cggggaataa atcctggtat
      661 ctgtttgatg ctgtccgtgg gaatgttgtt gtacgtgtaa cccggaatga tcgtgtcgcc
      721 gtctgaattg aggtagatgt aataataatc gttgtaaaga ttctctcttg tgcgcagcag
      781 tgaaatattc acccacgatt caacgccctc cacgaattcc ccgttgaggc ggagatcgag
      841 gccgtaggtg tagccatacg aaagattcgc gccgtaataa cggatgcgga cgtcgtccac
      901 ttcataggga ataagattgt ccaggtattt gtaatatgcc tccgtgatga aacggaacgg
      961 gcgcccccac cattgaaaag tccagtcgct gccggcaatg aaatgaacgg acgtctgcgc
     1021 cttcacatcc cggttgacat ggcccgtaaa atcacgcagc tcccggtaaa aaggaggctg
     1081 gtcatacagg cctgttgcga acttgaaggc gatgtctttt ttccagttgg gtttccagga
     1141 aaggcgtatg cgcggactta caatgagctg accgttgagg tcccagtatt gcgcgcggac
     1201 gccggcggtg acggaaaaat cggaagtgtc gcgaagcctg gtggaatatt tcagttgcac
     1261 gaatcccgag tagcgcatgg aattcagctg cgctgtgctc ttcaccacat tctgcaggtc
     1321 gatggtttcg tacggataat acgggatcga gtaaccggcc gaatcgatgt agttccattc
     1381 gctgagctga tcgttgatgc gctcgttctg cgcacgcagc ccgaagttcc acgtgtattt
     1441 gtcgctgtac gaatagcgcc acgctttgac ttctgcgttg ttcacccatg cgtcgaggta
     1501 attgcgcgca tggttgatga atgttcccac gccgcggttg aacgccacct ggccgaacgt
     1561 cggttttccg aaatccgcct cgagctgatc gatgtaatat tgtccctgca catcgaacgt
     1621 ttccgtttcg taggaattga aagaagacag gatgaacttc gagcgccagt gatggtgatc
     1681 gctgtagtcc agcgaaagcg catggaaatt cgtggtgaac gtatttattt cctgcccgtc
     1741 gaaataaacg gtaaagcgca gcgcgttgtt gatcgttccg aaatccgttt cacgtgtgga
     1801 gggaataagg ttgtatttgt tgtaggcata attgccgagg taattcagcg accattcatc
     1861 tgttatcgca taggaaagat aaacctgccc gtcgatgaag ttggtgaaat aatctccttt
     1921 cgtgtcgagc gaacgaagga catagcggtt ggttttattg cgcacgccca gcatccacga
     1981 aaaacggtga ttggtactcg aaccttctac ttccgcagtg aagccgagca ggcctgcgga
     2041 tgccgtaccg cggaattttg tcggtgtgcg gtacgtgaca tcaaggacgg aggacatttt
     2101 atcgccgtac ttcgcatcaa agccgccggc agaaaaatat acgttgtccg tcatgtcgat
     2161 gttcacgaag ctgagtcctt cctgctgacc tgcgcgcgtg agaaaaggcc ggtacacttc
     2221 gatatcgttc acataaacga gattctcgtc gaaatttccg ccgcgcacgg aatactgcgt
     2281 gctcagctca ttgggtgaaa aagcgccggc tgtatatttc aggacgttgg aaatatccgg
     2341 cccgttcggt gctggaagat gatcggcaat gtgcggaggg agcgggcggc cgaaatcatc
     2401 cttgtcgccg aaaacgttga tcacgttacc gacgtagttc gatttcattg tgaaattgag
     2461 cttcagcgta tctcctgcag aaaccgatat ttttttgctg ataccggtgt aacctacagc
     2521 gcggagaacg atcgtcactt cttttgcggc gggaacgttg aagcagaagt ttccgttggc
     2581 atccgtcatc gataatttgc tgctgccgat gtagccggcc tgcacatccg ccaggggttt
     2641 cccggtggaa tccttcacgg ttccgcatac ggtggccgac tgcgcgtgca ggaaagaaat
     2701 gcacgataaa agacaaagca cattcagcgc caggattttc acaaacaagc tgcggcgaaa
     2761 aatcagcata aagaaaacgg tgcgggaatt attgtcgttt gagttcgccg ttgttcagcg
     2821 cctggatgaa acgcgaccat gcgacaggat tgagaagatt gttcggcggt ttttgcccgg
     2881 cgtaataatt ctggttgacg cgctgctgca tttgtccctg gtaaaccatg ccgccgtcca
     2941 tcgccacgtt ctgcgccagc tgcaccattt cggcctgggc aagatttttc cgtgcgcgat
     3001 cgaggtcgtc ggagggaaca tttgccgtga ggaacgcgtt tttgaactgg tctttcgaag
     3061 gccatggata gacaaccgct tcgtgaagaa gaatggtgtc gagctttaac gcctgaacgt
     3121 ggttataatt gtccgacgtg atcgtatccg gcaaaataaa tgaagcccgc ttgtagccaa
     3181 gcgcataaaa atcgatcgtg tctttcgcct gcacgaccat ggagtaataa ccgttgaggt
     3241 tgctgtaaac gccgcgctcg gaatgccgca cgacgattgc cacgtaggga attccattca
     3301 ggctgtcggc gtcgagcaca atacccgaaa actggatgat cttgcgggtc gtgtcggtcg
     3361 ggatggtttg ttgggcagcg gaagaaaagg gaagaaggca cagcagtgcc gcacaggcgg
     3421 tcaggcagtg aaaaaatcct tttctcattg gtcaaattta cagaaaatac gggcagtccc
     3481 ggaatgtcct gccggccggc acaggttcaa attgttaaat atcatttatt gatatgacgc
     3541 aggttacggc cggggttgcc cgtgcggaat agttttgacg catgagattt tcagagaaat
     3601 tattgaagtt tacccgcata acgtcgggtc ttctgctcat ttttcttctg tccgtgccgg
     3661 cgctggtcgg tggttttgcg ttgatctccg ttccggatgg aagcacgttg cagatgaaaa
     3721 cagaattgct ccggcattca ccgttcagcg attttttcat tcccggcgtc gtgctttttt
     3781 ctgttaacgg gatactggcg atgatcgccg gtgtgttgtg catttcaggt aaatggatct
     3841 acccgcggct gacggttttc atgggtttct tcgtggtgat gtggatttcc atacagctgt
     3901 tcttcatcca cgaattcatc atcttccagc cgctttatct tgcgctcggg gcggttttga
     3961 tcgtgctcgg tttattgatg gaccggatga agaacaaagt ttcagcggca ggtaagcgtg
     4021 agattcagtc cgcaccacgc ccggtttcct gaaggaaaat aatacggctg cacgtgcatg
     4081 gaaagattat cgctcacatc gaaggtcatg aggtgcgcat cgactgcagc atcgatgatg
     4141 ttgagtgtat aaagcgcagc catgccgata aaacaaaggt cgcggttgcg ttcgtaataa
     4201 tttttcagc
//
LOCUS       DASTSF010000171         2594 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1392145,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000171 DASTSF010000000
VERSION     DASTSF010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2594
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1392145"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="VFU15_04960"
     CDS             <1..190
                     /locus_tag="VFU15_04960"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEU4717155.1"
                     /translation="VTIDVLNTTGQVILTQSAETPGGKQAFDIDLSAFDNGIYFIRCS
                     SISDNQYIVRTFKITLAR"
     gene            194..1126
                     /locus_tag="VFU15_04965"
     CDS             194..1126
                     /locus_tag="VFU15_04965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like protein"
                     /protein_id="HEU4717156.1"
                     /translation="MKRHFLFLFLIAASTAFAQAPQSVTYQGIARDLLGTPLTNQALG
                     LELTIHQGTPTGTITYQETFSTVTNQFGLYTVQMGGGTPVTGTFSAINWANGPYFLET
                     GMDITGGNNYVSAGTSQLLSVPYALYAETSGNGPAGPTGATGPTGSTGAAGVTGPTGA
                     AGASGTTGQNIYEVYGTGQLAVSTAVTTYTLIPGLTQTINIPANSMVYVHSDGGIQCT
                     GTGNSFSVVDIGLFVDGVVSSSAGQRRIVAANTTGLAQVITNWSLGRSYNLSAGNHTF
                     EVKVVSAGTGSTANVSSASAPQLQGVLTVTVIRL"
     gene            1158..1880
                     /locus_tag="VFU15_04970"
     CDS             1158..1880
                     /locus_tag="VFU15_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008976503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU4717157.1"
                     /translation="MKIAAVVPAYNESEAIASVVNELNSLAKTQPVNLTVIVVNDCST
                     DATGEIIDTLDCIALHLPVNLGIGGAVQTGFRYAYENDFDYAVQVDGDGQHPADEIPQ
                     LVAFAEANACDVVIGSRFIDKNGFQSTGMRRAGINYFSRLNRWLTGITVSDTTSGFRL
                     LNRKALNFVNENYPDEYPEPEAIVLYARHGMKIGEVPVKMRERRGGVSSINSVRAIYY
                     MWKVTLACLFSRIRKPGTTHHI"
     gene            1975..2352
                     /locus_tag="VFU15_04975"
     CDS             1975..2352
                     /locus_tag="VFU15_04975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717158.1"
                     /translation="MKKLIVLLSIFIPLFFFIACRHEPVLPDEQVSFTAQVQPIIRGS
                     CQHSGCHDTLSTSQDHRVIDSYEDMMADNRIVPGKPHSSQIYIDITASSGEDMMPTVA
                     YPRLTSEQIKLIYIWILQGAKNN"
     gene            2355..>2594
                     /locus_tag="VFU15_04980"
     CDS             2355..>2594
                     /locus_tag="VFU15_04980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717159.1"
                     /translation="MKKLIFPLAFVALLLGSCYYDNYDELYPGASLFTPCDTSGTISY
                     SQQIRNTLDNYCISCHSGSNPSGGVNLTDYATVNSY"
BASE COUNT          616 a          739 c          656 g          583 t
ORIGIN      
        1 cgtcaccatc gatgtgctca acacgaccgg gcaggtaatc cttacgcaga gcgcagagac
       61 tccgggcggg aagcaggcgt tcgatatcga tctttccgct ttcgacaatg gaatttattt
      121 catccgctgc agcagcattt cagacaacca gtacatcgtc cggactttca aaatcacatt
      181 ggcaagataa tttatgaaac gacactttct tttcctgttc ctcatcgccg cttccaccgc
      241 atttgcgcag gcaccgcaat cagtcactta ccagggcatt gcgcgcgacc tgctcggaac
      301 accgctgacc aaccaggcgc tcggtctcga gctgaccatt caccagggaa caccgacagg
      361 aacgatcacc taccaggaaa cgttttccac ggttaccaac cagttcggac tttacaccgt
      421 gcagatgggc ggaggaactc ccgtaaccgg aacattcagc gcgatcaact gggcgaacgg
      481 gccttatttc ctcgagaccg gcatggacat cacgggggga aacaattatg tttcggccgg
      541 aacatcgcag ctgctttccg ttccctatgc cctgtatgcc gaaacttccg gtaatggtcc
      601 cgcaggaccg accggcgcca caggaccaac cggcagtacc ggcgccgcgg gcgtaactgg
      661 tccgacagga gctgcaggcg ccagcggaac aaccgggcaa aacatttatg aagtttacgg
      721 aaccgggcag ctggcggtaa gtactgcagt gaccacctac acgcttattc ctggtcttac
      781 acagacgatc aatattcccg ccaacagcat ggtatatgtg cacagcgacg gcggaattca
      841 atgcaccggc acgggaaatt cattctcggt ggtcgacatc ggcttgttcg tggacggtgt
      901 tgtttcctcc tccgcgggac agcggcggat cgttgcggcg aataccaccg ggcttgcgca
      961 ggtgatcacg aactggtcgc tcggaagaag ctataatctt tctgcgggca atcatacgtt
     1021 cgaggtgaaa gtcgtttctg ccggaaccgg ttcaaccgcc aacgtgagca gcgcgtcagc
     1081 gccacagctg cagggcgtgc tcacggttac cgtgatccgc ctctgagcgg gatttttttt
     1141 cttcaacttc gttttgaatg aagatcgcag cggtggtacc ggcctacaat gaatcggaag
     1201 cgatcgcttc agtcgtgaac gagctgaatt ccctggcgaa gacgcaaccg gtgaacctca
     1261 ccgttatcgt tgtcaacgac tgctcaacgg atgcaaccgg tgagatcatt gatacgctcg
     1321 actgcattgc gcttcacctt cccgtaaatc tcggcatcgg cggcgccgta caaacgggtt
     1381 tccgctatgc gtacgaaaat gattttgatt acgcggtgca ggtcgacgga gacgggcagc
     1441 atccggcgga tgaaattccg cagcttgtcg cgttcgcgga agcaaacgcc tgcgacgttg
     1501 tcatcggttc gcgtttcatt gacaaaaacg ggttccagtc gaccggcatg cgccgggccg
     1561 gcatcaatta tttcagccgg ctgaaccgct ggctgacggg aattacggta tccgacacga
     1621 catccggctt ccgcctgctg aaccggaaag cgctgaattt cgtaaatgaa aattacccgg
     1681 atgaataccc cgagccggaa gccatcgttc tttatgcacg tcacggaatg aagatcgggg
     1741 aagtgcccgt gaaaatgcgg gagcgccgtg gcggagtatc ttccatcaac agcgtgcgcg
     1801 ctatttatta catgtggaaa gttacgcttg cgtgcctgtt ttcaagaata cgcaaaccgg
     1861 gaacaaccca tcatatttag aacgcatcta aatttgaacc gggaaattta ttttaatgcg
     1921 cactgagcat ataagtgtat ttacacgcaa tacctttgta taaattatta tcacgtgaaa
     1981 aaattgatcg ttcttctttc catcttcatc cccctgtttt tctttattgc atgccggcac
     2041 gaacctgtgc ttccggacga acaggtaagc ttcacggccc aggtccaacc gatcatacgc
     2101 ggaagctgcc agcattccgg ttgtcatgac acgctgtcca catcgcagga ccaccgcgta
     2161 atcgacagct atgaagatat gatggcagac aaccgcatcg ttcccgggaa gccgcacagc
     2221 tcgcagatct acatcgacat caccgccagc tcgggcgagg acatgatgcc gaccgttgcc
     2281 tatccccgtc ttaccagcga gcagatcaaa ctcatttata tctggattct ccagggtgca
     2341 aaaaacaact gatcatgaaa aaactgattt tcccgctggc ctttgtcgcc ctgcttctcg
     2401 gcagttgtta ctacgacaac tacgacgagc tttacccggg cgcatctctt ttcacgccct
     2461 gcgatacttc cggcacgatc tcttattcgc agcagatccg gaacacgctc gacaattatt
     2521 gcatttcctg ccattccgga tcgaacccga gcgggggcgt taacctgacg gactatgcaa
     2581 ccgtcaacag ctat
//
LOCUS       DASTSF010000172         4349 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_989575,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000172 DASTSF010000000
VERSION     DASTSF010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4349
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_989575"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..672)
                     /locus_tag="VFU15_04985"
     CDS             complement(<1..672)
                     /locus_tag="VFU15_04985"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013453975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HEU4717160.1"
                     /translation="MKKTFFRTIVLSDIHLGTSSSKAKEVVHFLRSHKCETLILNGDI
                     IDGWQLKKSGAWKKKHSKFFKVILRRAAKGTNIIYIRGNHDDFLDEVLPFSLGNFSIR
                     RDCYIESAGKKYYVVHGDIFDTITTKLKWVAKLGDVGYTFLLWLNKHYNKWREKRGLP
                     YYSLSQVVKAKVKGAVSFISDFETQLAAVAKARHCDGVICGHIHTPADKMIGKIHYLN
                     SGDWVE"
     gene            1007..1450
                     /locus_tag="VFU15_04990"
     CDS             1007..1450
                     /locus_tag="VFU15_04990"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEU4717161.1"
                     /translation="MTNIIKFGPRRSVFAHPFFRSTFSDLWDDTFTGKEFAAYVPAVN
                     IHEEDKSWHIEVSAAGFAKEDFRISLEKDVLTISADKKEESGNEGRNYHRREFRHGSF
                     SRSFRLMKDSVDEKGITASYENGILTIVVPKKGEVKDESMEIRVV"
     gene            1593..2381
                     /gene="surE"
                     /locus_tag="VFU15_04995"
     CDS             1593..2381
                     /gene="surE"
                     /locus_tag="VFU15_04995"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008866119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HEU4717162.1"
                     /translation="MTRKPLILVTNDDGIHAPGIVALVETAMSFGDVVVVAPDKPQSG
                     MGHAITINSTLRVERTNAHPGAESWSCSGTPVDCVKMAVNKLLPSKPALVLSGINHGA
                     NHSINIIYSGTMSAAMEGAIEGIPSAGFSLLDYSINADFSASKVIAEKVIAFLLKKGL
                     PGGVCLNVNIPKLHIDEIRGIRVCRQANGTWVEELDERTDPSGRKYYWLTGSFKNFEP
                     EATDTDVWALSNNYVSVVPAGTDLTAHSALEKIHPLNGAHPGKR"
     gene            2378..2677
                     /locus_tag="VFU15_05000"
     CDS             2378..2677
                     /locus_tag="VFU15_05000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717163.1"
                     /translation="MKKKFDNVILGLLIGLLAPLAALYIYYLFTYRYQTSFSGFLQYF
                     NSFHIVVASISLACYASNLPLFFLFVWRERYQSARGVLTATIIYTCWVVYEKFFV"
     gene            2683..3783
                     /gene="lpxB"
                     /locus_tag="VFU15_05005"
     CDS             2683..3783
                     /gene="lpxB"
                     /locus_tag="VFU15_05005"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014201809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HEU4717164.1"
                     /translation="MKYYLISGEASGDLHASNLVRELKQLDPAAEFRGFGGDLLAKEG
                     MHLVRHYRDLAFMGFAEVILHLRTILNNISFCKNDIREWKPDAVILVDYPGFNLRVAP
                     FVKELGIKVFYYISPQVWAWKQSRVFRIREVVDRMFCILPFEKDFYAKFDFEVDFVGH
                     PLLDAIAREENSEPALADDGRKIIALLPGSRGQEIKTMLPVMLSAASRFPEFRFVIAG
                     APSQPEKFYRGLMNGNSTEIVFGKTYALLRQSHAAIVTSGTATLETALHAVPEVVCYR
                     GSTVSYQIAKRLVKVDYISLVNLVMGKEVVKELIQSEMNAGTISAELKKITADTNSRQ
                     KMLDDYAVLREKLGGPGASRRTAELIIKNLGA"
     gene            3780..>4349
                     /locus_tag="VFU15_05010"
     CDS             3780..>4349
                     /locus_tag="VFU15_05010"
                     /inference="COORDINATES: protein motif:HMM:NF020075.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HEU4717165.1"
                     /translation="MKKLATFFFLLCCLSLAAGNINVRVLSSMNVFSANISVYAGSYD
                     CYINDIQQPDSIATQVFQLSVVNDSLEIRIPGDTLGLFSSFRIVARTDSSAFKIKPIR
                     PMSGTRVYDHSLKITVRDGKLLCINEVELEHYVAGVVESESGGRTAKEFYKVQAILCR
                     TYALAHMSRHAPEGFDLCDGVHCQAYRGRP"
BASE COUNT         1014 a         1116 c         1155 g         1064 t
ORIGIN      
        1 ttccacccag tcgccgctgt tcaggtaatg gatcttcccg atcattttat cagcgggggt
       61 atggatgtgg ccgcagatca cgccgtcgca gtgacgcgct ttggccacgg ccgcaagctg
      121 cgtttcgaaa tcggaaatga agctcacggc gccttttact ttagccttca ccacctgcga
      181 aagactgtaa taaggaagcc cgcgtttttc gcgccatttg ttgtagtgct tgttcagcca
      241 gagcaggaac gtgtagccga cgtcgccgag cttcgcgacc catttaagtt tcgtggtgat
      301 cgtgtcgaaa atatcgccgt gcacgacgta atattttttc ccggccgatt cgatgtagca
      361 gtcgcggcgg atggaaaaat taccgagcga aaaaggcagt acttcgtcga ggaaatcgtc
      421 gtggttgccg cggatgtaga tgatgttcgt tcctttggct gcgcgacgca ggatgacctt
      481 gaagaattta ctgtgcttct ttttccaggc cccggatttt ttcagctgcc acccgtcgat
      541 gatgtcgccg ttgagaataa gcgtttcgca tttatggctg cggaggaaat gaacgacctc
      601 tttcgctttg gaggacgacg ttcccaggtg gatgtctgag agcacgatcg tccggaagaa
      661 tgtttttttc acctctggag ggttttgcaa atttccattt gccacatgaa ctccggatga
      721 agggaagctg atgcgtgtat cacgtttttg ttgtgccggt gtaaactgca tacgctgggt
      781 tcagcttctg ttcacttagg ctgacggata atggcgcgca gttatttccg gcgttaggaa
      841 attttggcaa tgcttttccc gtaaaagctg aaaaaatttc aggtttccca atgaaacgct
      901 gacggaatgg ctgttaagca atgtgaaaaa tgcgttggta cggaaaatga catgatgtac
      961 actgaaacga taaccaacaa tcaataacca ataaaacata attgccatga ccaacattat
     1021 taaattcgga ccacgccgtt cagtgtttgc gcacccgttc ttccgttcca ccttttccga
     1081 tctgtgggac gatacgttca ccgggaaaga gtttgcagcg tatgttccag ccgtaaacat
     1141 tcatgaagaa gataagagct ggcacatcga agtttcggcc gcaggttttg cgaaagaaga
     1201 tttccgcatc agtcttgaaa aagacgtgct gacgatcagt gccgataaaa aagaagaaag
     1261 cgggaacgaa ggacgcaact accatcgccg cgagttccgt cacggttctt tcagccgcag
     1321 cttccgcctg atgaaagaca gtgtggacga aaaaggaatt acagcctctt atgagaacgg
     1381 catcctgacg atcgtcgttc ccaaaaaagg agaggtaaaa gacgaaagca tggaaatccg
     1441 ggttgtgtga aggggatgag cgaccggatt taaccgaagc cgccacgtaa aaagcgtggc
     1501 tgttttcgtt ccaacacttt ttaacaggcc gtgtttgatt actcgccggc cgtattccct
     1561 gcaggggtgc ttccgctcct aactttgcaa gaatgacccg taaaccactc atccttgtta
     1621 cgaatgacga cggcatacac gccccgggaa ttgttgcgct cgtggaaacg gccatgagct
     1681 tcggcgacgt ggtcgttgtg gcgccggaca agccgcaatc aggaatgggc catgccatca
     1741 cgatcaattc gacgctgcgc gtggaaagaa cgaatgcaca tccgggcgcg gaaagctgga
     1801 gctgcagcgg cacgccggtc gactgcgtga aaatggccgt gaacaagctg cttccttcaa
     1861 agcccgcgct ggtgctttca gggatcaacc acggcgcgaa tcattccatc aacatcattt
     1921 attccggcac gatgtcggca gcgatggaag gcgcgatcga aggaattcca tccgccggtt
     1981 tttcgctgct cgattattcg atcaacgcgg atttttccgc ttcgaaggtg atcgctgaaa
     2041 aagtgatcgc tttcctgctg aagaaaggat tgcccggcgg cgtttgcctc aacgtcaata
     2101 tcccgaagct gcacatcgat gagatccgcg gtatccgcgt atgccgccag gcgaacggaa
     2161 cctgggtgga agagctcgac gaacggacgg atccttccgg aaggaaatac tactggctta
     2221 cgggatcatt taaaaatttc gaacccgaag ccaccgacac ggatgtctgg gcgctttcga
     2281 ataattatgt ttccgtcgtt cctgcaggaa ccgatctcac cgcgcattcg gcgcttgaaa
     2341 aaatccatcc gctgaatgga gctcatcccg gaaaaagatg aaaaagaaat tcgataacgt
     2401 gatcctcgga cttttgatcg gattgctcgc gcccctggca gcgctgtata tctattacct
     2461 gttcacgtat cgttaccaga cttccttctc cggctttctg cagtatttca attctttcca
     2521 tatcgtcgtc gcgtcgatca gccttgcctg ctacgccagc aacctcccgc tgttttttct
     2581 ttttgtctgg cgcgaaagat accagtcggc gaggggcgtg ctcacagcca cgatcatcta
     2641 cacctgctgg gttgtctatg aaaaattctt cgtctgacgg aaatgaagta ctacctcatt
     2701 tccggagaag cttcaggcga cctgcacgca tcgaaccttg tgcgtgagct gaagcagctc
     2761 gatccggctg cagaattccg cggtttcggc ggcgacctgc tggcgaaaga gggaatgcat
     2821 ctcgtgcgcc attaccgcga tcttgcgttc atgggttttg cggaagtcat tcttcacctc
     2881 cgcacgatcc tcaataatat ttcattctgc aaaaacgata tccgggaatg gaaacccgat
     2941 gcggtcatcc tcgtcgatta cccgggcttc aacctgcgcg tggctccttt cgtgaaggag
     3001 ctcgggataa aagtttttta ttacatctct ccgcaggtgt gggcctggaa gcaatcgcgt
     3061 gtgttcagga tccgcgaagt ggtcgaccgc atgttctgca tccttccctt tgaaaaagat
     3121 ttttatgcga agttcgattt tgaagtggat ttcgtgggac atcctttgct tgacgcgatc
     3181 gcaagggaag aaaacagcga gcctgcactg gcggatgacg gccggaaaat aatcgccctg
     3241 ctgcccggga gccgcgggca ggagataaag accatgctgc cggtcatgct cagcgccgcc
     3301 tcccgtttcc ctgaattccg ttttgtcatt gcaggtgcgc cttcccagcc ggaaaaattt
     3361 taccgcggcc tgatgaacgg gaacagcacg gagatcgttt tcggaaaaac atacgcgttg
     3421 ctgcggcagt cgcatgcggc gatcgtcact tccggaacgg ccacgctcga aacagctttg
     3481 cacgcggtgc ctgaagttgt ctgctacagg ggaagcacgg tttcctatca gatcgcgaaa
     3541 cgcctggtga aggtggatta catttccctc gtcaacctcg tgatgggaaa agaagtggtg
     3601 aaggaactga tccagtcgga aatgaacgcc ggtacaattt ctgctgagct gaaaaagata
     3661 accgcggata ccaacagccg gcagaaaatg ctggacgatt atgctgtcct ccgtgaaaag
     3721 ctgggcggtc ccggtgcttc gcgacggacg gcagagctga tcatcaaaaa tctcggtgcg
     3781 tgaagaaact ggcgaccttt ttctttctcc tttgctgcct ttcgcttgcg gccggcaaca
     3841 tcaatgtgcg cgtgctttcg tctatgaatg ttttttccgc gaacatttcc gtttacgccg
     3901 gttcgtatga ttgttacatc aacgatattc agcagcccga ttccattgcc acgcaggtgt
     3961 tccagctttc cgttgtaaat gattccctgg aaatacgaat tcccggcgat acgctcggac
     4021 ttttttcatc gttccgcatc gtcgcccgca ctgattcgtc cgcattcaag atcaaaccca
     4081 tcagaccgat gagcggaacg cgcgtgtatg atcattcgct gaagatcacc gtgcgcgacg
     4141 ggaagctgct ttgcatcaac gaagttgagc tcgagcatta cgtggcgggc gtcgtggaat
     4201 ccgaatcagg cggaagaaca gcgaaggaat tttacaaagt gcaggccatc ctctgccgca
     4261 cgtacgcgct cgcgcacatg tcgcggcatg cgccggaagg tttcgatctc tgtgacggtg
     4321 ttcattgcca ggcttaccgc gggcgtcct
//
LOCUS       DASTSF010000173         3077 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_112356,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000173 DASTSF010000000
VERSION     DASTSF010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3077
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112356"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(15..1112)
                     /gene="dprA"
                     /locus_tag="VFU15_05015"
     CDS             complement(15..1112)
                     /gene="dprA"
                     /locus_tag="VFU15_05015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HEU4717166.1"
                     /translation="MAFDERVFQVALSMIPGIGPVTSKKLITRFGSAPAVFRAKKNEW
                     RDVPFAGKHLAAMVKDKSVLLRAEKEIAFAEKNNIRLIFFSDKEYPYRLKQCFDGPYL
                     IYYRGNAPLNPARVVSIVGTRRASEYGRMLTEKLVEDLSACSVTVVSGLAFGIDILAH
                     KASLKYGAPTIGVLGHGLDMLYPVGHKATAKKMEENGGILSDFPSETIPDKENFPLRN
                     RIVAGMSDATIVVQSGASGGSMITAEFANNYNRDVFAFPGAVGDALSAGCHSLIRQNK
                     AALIESADDVLKFMGWERKEEKPKQSLMHIGTTPEQETLLTLLRGQGNVHIDDLVLAS
                     GFSQGKTSSILLELEFAGAVKSLPGKFYRLN"
     gene            1209..>3077
                     /locus_tag="VFU15_05020"
     CDS             1209..>3077
                     /locus_tag="VFU15_05020"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU4717167.1"
                     /translation="MSNRKFFLVFPFLLFSVFCFSQSSYIKGTVRDSSSGDPLIGVII
                     RSSAGGGAVSDVNGGYVIKAKEGKQVVTFSYTGYTTDSVSVDVTPGATLQLDIKMSNT
                     SRQLSIVVVSSGRYEQDIRQVPVSMEVLKPKLIDDKNTTTMETIMDQVPGVNMTDGQA
                     NIRGGSGFSYGAGSRVLLVVDDMPMLSGDAADVKWNYLPIENLSQVEVMKGASSALFG
                     SSALNGVIQFRTAYPTSTPQTTISMTSGFYGDPRRPELRWWKTSNPTFAGMNFCHSRQ
                     IGNLDLVVAGHIYNDEGYRYLETEQRERLNINTRYRFKKISGLSAGVNANMMRTTGGL
                     FLLWYNDSLAYIPADSTIQIYKNYRWNIDPFITYYGPKGHRHSLRGRYFRTNNINDKD
                     QGSLADFWYGEYQYQYKFKNKMTVTAGLVGSYTTVKADLYGDHIASNVAGYAQFDRRF
                     WDRLFVTVGVRGEYNRVDTATTEFHMNLSKNDTLKLPFRPVMRVGVNYQVNEGTFLRI
                     SYGQGYRFPCVAEKFIRTDAAGLEIYPNPSLQPETGQSAEIGVKQGVRVGQWKGYADL
                     SIFWMQYENMMEFAFGQFGKPTVDPLFGLGFRSENIGSTRTRGVDFSLMGEGKIGPV"
BASE COUNT          699 a          889 c          821 g          668 t
ORIGIN      
        1 gtttgagccc ggctctaatt cagcctgtaa aattttcccg gcaatgattt cacggctccc
       61 gcgaattcca gctcgagcag gatggacgat gtttttccct gcgaaaaacc cgaagccagc
      121 acgagatcgt cgatgtgcac attaccctgc ccgcgcagca gcgtaagcag cgtttcctgt
      181 tccggcgtcg tgccgatgtg catgagcgac tgtttcggtt tttcctcttt ccgttcccag
      241 cccatgaatt tcagcacatc gtccgccgat tcgatcagcg ctgctttgtt ctgccggatg
      301 aggctgtggc agcctgccga cagcgcgtcg ccgacagcgc cgggaaatgc gaacacgtcg
      361 cggttgtaat tattcgcgaa ctccgcggtg atcatggagc cgccgctcgc accggactga
      421 acaacgatcg tcgcatcgct catgccggca acaatgcggt tgcgcagcgg gaaattttcc
      481 ttatcgggaa tcgtttcgct gggaaaatcg gaaagaatgc ccccgttctc ctccattttt
      541 ttcgccgtcg ctttgtgccc gaccgggtac agcatgtcga ggccgtgacc gagcacaccg
      601 atcgtgggcg cgccgtattt cagcgacgct ttgtgcgcaa ggatgtctat gccgaacgca
      661 agcccgctca ccaccgtcac gctgcaggcg gaaagatcct ccacgagctt ttccgtgagc
      721 attcttccgt attcgcttgc gcgacgcgtg cccacaatgc tcaccacgcg cgcaggattg
      781 agcggcgcat ttccccggta ataaatgagg tacggtccgt cgaaacattg cttcaggcgg
      841 taaggatatt ctttgtcgct gaaaaaaatg agccggatat tattcttttc cgcaaacgca
      901 atttcctttt ccgcgcgcag gagaacagac ttatccttca ccatcgcagc gaggtgtttt
      961 cctgcaaaag ggacatcgcg ccattcgttt tttttcgccc tgaaaaccgc cggggcgctg
     1021 ccgaaacggg tgatgagttt tttggaagtg acgggcccga tgcccggtat catacttaga
     1081 gcgacctgga agacacgttc gtcgaaagcc atattgtacc tgttaacctt ttcttataca
     1141 ttgcaaatga taaacttaaa atcagctaaa acataaaccc caaaaaggga cggcaaaaat
     1201 acaaaagcat gtcaaaccgg aaattttttc ttgttttccc cttccttctc ttttctgttt
     1261 tctgtttcag ccagtcgtcc tacatcaaag gcaccgtacg cgacagctcc tcgggcgatc
     1321 cgctgatcgg ggtgatcata cgttcttcag ccgggggcgg tgcggtttcg gatgtgaacg
     1381 gcggttatgt catcaaagcg aaagaaggaa agcaggtggt gacgttcagc tacaccggct
     1441 acaccacgga ttctgtttcc gtcgacgtaa cgccgggcgc cacgctgcag ctcgacataa
     1501 aaatgagcaa cacctcgcgg cagctgagca tcgtggtcgt gtcatcaggc cgttatgaac
     1561 aggacatccg ccaggtgccc gtttccatgg aagttttgaa gccgaagctc atcgacgata
     1621 agaacaccac gacaatggaa accatcatgg accaggtccc cggcgtgaac atgaccgacg
     1681 gccaggcgaa catacgcggc ggaagcgggt tcagctacgg cgccggttcc cgcgtgctgc
     1741 tcgtggtcga cgacatgccc atgctttccg gcgatgcggc cgacgtgaaa tggaattacc
     1801 tgccgatcga aaatctttcg caggtggaag tgatgaaagg cgcatcgtcc gcgctcttcg
     1861 gttcatccgc gctcaacggt gtgatccagt tcaggaccgc ttacccgacg agcacgcccc
     1921 aaacgacgat ctcgatgacg tcgggatttt acggcgatcc gcgccggccc gaattgcgct
     1981 ggtggaaaac gagcaacccc acgttcgcag gaatgaactt ctgccattca cgccagatcg
     2041 ggaacctcga cctcgtggta gcgggacata tttacaacga cgaaggttac cgctacctcg
     2101 aaaccgaaca acgcgaacgg ctcaacatca atacacgcta ccgtttcaaa aagatctccg
     2161 gcctcagcgc cggtgtcaat gcgaacatga tgcgcacgac gggcggactt ttccttctct
     2221 ggtacaatga ttcgctcgcg tacatcccgg cggacagcac gatacagatc tacaaaaatt
     2281 accgctggaa catcgatccg ttcatcacct attacggacc gaaaggtcac cgccattcgc
     2341 tgcgcgggcg ttatttccgg acgaacaaca tcaacgacaa agaccagggc tcgcttgcgg
     2401 atttctggta cggcgaatac cagtaccagt acaaattcaa aaacaaaatg accgttaccg
     2461 cgggccttgt gggatcgtac accacggtga aagccgacct ctacggcgat cacatcgctt
     2521 cgaatgtcgc ggggtacgcg cagttcgacc ggcgcttctg ggaccggctg ttcgtcactg
     2581 tcggtgtgcg cggcgaatac aaccgcgttg acaccgcgac gacggaattc cacatgaacc
     2641 tttcgaagaa cgacacgctg aagctcccgt tccgtcccgt gatgcgcgtc ggcgtgaatt
     2701 accaggtgaa cgaaggaacg ttcctccgca tctcgtacgg gcaaggttat cgcttcccct
     2761 gtgtcgcgga aaaattcatc cgcaccgatg cggcgggact ggagatctac cccaatcctt
     2821 cgctgcagcc ggaaacgggg cagtcggccg agatcggcgt gaagcagggc gtacgcgtgg
     2881 gccaatggaa aggctacgcg gacctttcca tcttctggat gcaatacgag aacatgatgg
     2941 aattcgcgtt cggacagttt ggaaaaccca ccgtcgatcc gctctttggt cttggtttcc
     3001 gttcggaaaa tatcggcagc acacgcacac gcggcgtcga tttctcactg atgggcgaag
     3061 ggaagatcgg gcccgtc
//
LOCUS       DASTSF010000174         4291 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1147098,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000174 DASTSF010000000
VERSION     DASTSF010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4291
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1147098"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(237..833)
                     /locus_tag="VFU15_05025"
     CDS             complement(237..833)
                     /locus_tag="VFU15_05025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717168.1"
                     /translation="MILTFETLNEKHEHAMRMFLHESGLGHVAAEWKRSLENARELSV
                     SVPHAVYDAFLDELAAGKGISKFKRAGIRSLLLNEGETIYGAVETADPLPEKGVSKGS
                     VQLEYGPFVGATHEALKHLETETAHAGETYRFRLLRIPGIYVMAAWLHGEKQNLFMPI
                     GQAPKSLKPYTLYSEKDFLALLKTLAREKLDFEKKNPE"
     gene            complement(846..1409)
                     /locus_tag="VFU15_05030"
     CDS             complement(846..1409)
                     /locus_tag="VFU15_05030"
                     /inference="COORDINATES: protein motif:HMM:NF023802.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HEU4717169.1"
                     /translation="MALSSYVINTRKVIMDRPILESLLQKLAIEKTLPYTCLHQEQTE
                     WCWVAVTAAVNGFYGRTLQQCDIANNCLDQSTCCVDGSSAACNTPWYLNRSLSDYNSL
                     NAMTAGKMEFASVQSEIDNSNPVCLRIGWNGGGGHFVAVYGYNSTDEYITIGDPWFGV
                     NVRDYNTFPTQYHAGGTWTTTYTTKHF"
     gene            complement(1627..2628)
                     /locus_tag="VFU15_05035"
     CDS             complement(1627..2628)
                     /locus_tag="VFU15_05035"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEU4717170.1"
                     /translation="MPLTVKSSSHNTIHSNGYTAQDFHSAELVEESRVMHTDFAEGKM
                     MHWYFDGIRMAHSAWNYTRSETTEWSGALNVVTLYFNLKGKFSLHAGKNHKPLVLESH
                     EHNMFATGDEPFRSLTGDEELKHENFLIQLTHESFAKLTQDAGDNITRFAEKISGEKP
                     VILSGRNLHMNVALHAAIREITGCRFTGGLKKIFLLSKCLEVLVLQAEAYNNAQGQKI
                     IYCKTDYDRERIRFAHDYLLGHMYAPPSLTELARVAGINEFKLKRGFRELFGNSVFGY
                     LAEHRLGLARRDLQEGKKTATEIAFDLGYSSLQHFSAAFKKKYGISPKELVRQGRKK"
     gene            2726..2941
                     /locus_tag="VFU15_05040"
     CDS             2726..2941
                     /locus_tag="VFU15_05040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717171.1"
                     /translation="MIEVFKTNVDSEEKAEEAMKLLNEKLAGCEISFDVQDCDHVLRI
                     DSQEEHLTGKIIALLSGMGFQCETLSD"
     gene            2977..3639
                     /locus_tag="VFU15_05045"
     CDS             2977..3639
                     /locus_tag="VFU15_05045"
                     /inference="COORDINATES: protein motif:HMM:NF022441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2807 domain-containing protein"
                     /protein_id="HEU4717172.1"
                     /translation="MIEVKGNGNVVTREIPVSSFIRLHLSAKGLIELIQSDEEKVVVE
                     ADENLQDYFEAVNAGRTLYVTSEAKFRLPVFTKCVIRVYLRQIDMLYVRCDKGDVTCA
                     GTLAFTEPLEIKIQSIGNTELNIDAPAVKVLSQCQGNVVLKGRCGSITIKNQNEGDFS
                     SRDLLAGELTMKNMSVGNIELYADRKISISHYGEGYVHYYGDAALKDVKQYGNGQIRH
                     MK"
     gene            complement(3699..>4291)
                     /locus_tag="VFU15_05050"
     CDS             complement(3699..>4291)
                     /locus_tag="VFU15_05050"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEU4717173.1"
                     /translation="CTSSSSSIAVTVIDACCSSGNCGHNNGVAICHDGHTICVSPNAV
                     AAHLAQGDQLGACDNNDHGHGHGYGDNDDHGHGHGDGDDHGHGHGNDDHGHGHGDRIA
                     YIQGDSTNTVVNAYPNPFDNSVNVQVSVTNDEPVRVEVKDIFGRTIAVIQDGNLSAGT
                     YQYTWTPGSDVASGVYFIDTVCGGEHQVTKLVYQGK"
BASE COUNT          994 a         1054 c         1208 g         1035 t
ORIGIN      
        1 ccaatttaag atttttctct gaacagtttc ctttcaaaaa aatctcaaaa cagcttttct
       61 aaacggaaaa caccccatgc cggcaagcta acctgccgaa gcaaaaacga aagcaggcaa
      121 cattattcgg gagaatttaa atagaattcg cggcgaactc tgccggaccg caggcgggaa
      181 gagagcaaaa aaatgcacgg ttgatgcgct gcaaagtaaa cgcaacgcga acccgcttat
      241 tccgggtttt tcttttcgaa gtcgagcttt tcccgggcca gagttttcag cagcgcgagg
      301 aagtccttct cgctgtaaag ggtatacggc ttcagcgatt tcggtgcctg gccgataggc
      361 atgaacaggt tctgcttttc cccgtgcagc catgcggcca tgacataaat tccgggtatg
      421 cggagcaggc ggaaacgata cgtctcaccg gcatgggcgg tttccgtttc aaggtgtttg
      481 agcgcttcgt gggtggcgcc gacgaacggt ccgtactcga gctgcacgct gcctttgctt
      541 actccttttt caggaagcgg atcggctgtt tccacagcac cgtaaatggt ttcgccttcg
      601 ttcagcaaaa gagaacgtat cccggcccgt ttgaacttgc tgatgccttt gccggcggcg
      661 agctcgtcga ggaaggcatc gtagacggcg tgaggaacgg agactgataa ttcacgggcg
      721 ttttcgagcg aacgtttcca ctctgctgca acatgtccca gtcccgattc gtggaggaac
      781 atccgcatgg cgtgttcgtg cttttcattg agcgtttcaa aagtgagtat catgaccgtg
      841 cggatttaaa aatgttttgt cgtgtaggta gttgtccatg tgccgcctgc gtggtactgg
      901 gtgggaaagg tgttgtagtc gcggacgtta acgccgaacc agggatcgcc gatggtaatg
      961 tattcatccg tggaattgta cccgtaaaca gcgacgaaat gtccgccgcc tccgttccac
     1021 ccgatgcgaa ggcaaacggg atttgaattg tcaatttcac tctgcacgga agcgaactcc
     1081 attttgccgg ctgtcatggc attgagcgaa ttgtaatcgc ttaacgatct gttcaggtac
     1141 cacggcgtgt tgcaggccgc agaagatccg tcgacgcagc aggttgactg gtcaaggcag
     1201 ttgttcgcaa tgtcgcactg ctggagcgtg cggccgtaaa atccgttgac cgccgcagtg
     1261 acggctaccc agcaccattc tgtctgttcc tggtgaaggc atgtgtacgg cagtgttttt
     1321 tcgatcgcca gtttttgcag gagcgattcg agtatgggcc ggtccatgat gaccttgcgg
     1381 gtgttgatca cgtaggatga aagtgccatg tgttgcagtt tggttgggtt atttagcaag
     1441 ggtacaacag tttttccgga gtagtcggat ataaacgttt gtaaaacgat taatgcccgc
     1501 gtgagatagg gttcccagga gagcggactt ctgaatcacg gacgacagtt catctttttt
     1561 caagaacagt catgaaaatc gccgaaggaa caggagagcc ggaattcagc tgagcagaaa
     1621 ggcgggttat ttttttcgtc cctgccgcac gagctctttc ggcgatatcc cgtatttctt
     1681 tttgaatgct gcgctgaaat gctgcaggga agaataacct aaatcgaacg caatttccgt
     1741 cgccgttttt ttcccttcct gcaggtcgcg gcgcgcgagt ccgagccggt gttctgcgag
     1801 gtaaccgaaa acggaatttc cgaacagctc gcggaagccg cgcttgagct tgaattcatt
     1861 gatgccggcc acacgcgcga gctccgtgag cgaaggcggc gcgtacatgt gcccgagcag
     1921 gtaatcgtgc gcgaagcgga tgcgttcgcg gtcgtaatcg gttttgcagt aaatgatctt
     1981 ctgcccctgc gcattgttgt acgcttccgc ctggaggacg agcacctcga ggcatttcga
     2041 caagaggaat atttttttga gtccgccggt aaacctgcac ccggttatct cgcgtatcgc
     2101 tgcgtgcagc gccacattca tgtgcaggtt gcgcccggaa agaataaccg gtttttcgcc
     2161 ggagattttt tcggcaaagc gcgtgatgtt gtcgccggcg tcctgcgtca gctttgcaaa
     2221 tgattcgtgc gtgagctgga tgagaaaatt ttcatgcttc agctcttcgt cgccggtcag
     2281 tgaacggaac ggctcgtcgc cggtcgcgaa catgttgtgc tcgtggcttt ccaggacgag
     2341 cggcttgtgg tttttcccgg catgcaggga aaattttcct ttcaggttga aataaagcgt
     2401 gacgacattc agcgccccgc tccattcggt ggtttcgctt ctcgtgtaat tccacgcgct
     2461 gtgcgccatg cggatgccgt cgaaatacca gtgcatcatc tttccttctg caaaatccgt
     2521 gtgcatcacg cgcgattctt ccaccagctc tgccgaatga aaatcctgcg cagtgtagcc
     2581 gttgctatgg atcgtgttat gcgacgaaga ttttacggtg agcggcatta ttccgggtaa
     2641 tgtaagtttt tatccccctg ctgtaagccc gtgaaaggag aggcaaagca tctttgcgac
     2701 atcaaaatta agtaaaacaa caaccatgat cgaagttttt aaaacgaatg tcgacagcga
     2761 ggagaaagcg gaagaggcga tgaagctgtt gaacgaaaag ctggccgggt gcgagatcag
     2821 cttcgatgtg caggactgtg atcacgtgct ccgcatcgat tcgcaggaag aacatctgac
     2881 cgggaagata atagcgctgc tttcggggat gggatttcaa tgtgaaacgc tcagcgactg
     2941 acaacggtta tggttaactt caaaaaacta agacaaatga tcgaagtaaa aggaaacggg
     3001 aacgtcgtta cccgtgaaat acccgtgagc tcatttatac gtcttcatct ttccgcgaaa
     3061 ggactgatcg agctcatcca gtcggacgaa gaaaaagtag tcgtggaagc ggatgagaat
     3121 ctccaggatt atttcgaagc ggtgaacgcg gggcgtacgc tttacgtgac ctcggaagcg
     3181 aaattccggt tgccggtctt tacgaaatgt gtgatccgcg tttatctccg gcagatcgat
     3241 atgctttacg tgcgctgcga caaaggcgac gtgacgtgcg cgggaacgct tgctttcacc
     3301 gaaccgctcg aaataaaaat tcagagtatt ggaaatacgg agctgaacat cgatgcgcct
     3361 gcggtgaaag tgctcagcca gtgccaggga aacgtcgtgc tgaaaggacg gtgcggaagc
     3421 ataaccatca aaaaccagaa cgaaggagat ttttcatcgc gtgacctgct tgccggggag
     3481 ctgacgatga aaaatatgtc ggtgggaaat atcgagctgt atgccgacag gaaaatttcc
     3541 atttctcatt acggcgaagg gtatgttcac tattacggag atgcggcgct gaaagacgtg
     3601 aagcaatacg gaaacgggca gatccgtcac atgaaatagg caaacgaaaa gaggctgtct
     3661 tacgggacag cctccttcga caagcggaaa gcaacctgtt actttccctg gtaaacaagc
     3721 ttcgttacct ggtgctcgcc accgcagaca gtgtcgatga aataaacacc gctggccacg
     3781 tcgctgccgg gcgtccaggt gtactggtac gtgccggcgc tgagatttcc atcctggatg
     3841 actgcgatcg tgcggccgaa aatgtctttc acttcgacgc gaacgggttc gtcattggtc
     3901 actgacacct gcacattgac cgagttgtcg aacggattcg ggtaagcatt taccacggtg
     3961 ttcgtggaat cgccctggat gtacgcgatg cggtctccgt gcccgtgacc gtggtcatcg
     4021 ttaccgtgac catgtccatg atcgtcgccg tcgccatgcc cgtgcccgtg atcatcgttg
     4081 tcgccgtacc cgtgcccgtg cccgtgatcg ttattgtcgc aagcgccgag ctggtcgcct
     4141 tgtgcaaggt gtgcagcaac cgcgttcggg ctgacgcaga tcgtgtggcc gtcgtggcag
     4201 atggctacgc cgttgttatg cccgcagttg ccgctgctgc agcacgcatc tataaccgta
     4261 actgcaatcg acgaagaaga cgaagtgcat c
//
LOCUS       DASTSF010000175         7879 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1391121,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000175 DASTSF010000000
VERSION     DASTSF010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 7879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7879
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1391121"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..492)
                     /locus_tag="VFU15_05055"
     CDS             complement(<1..492)
                     /locus_tag="VFU15_05055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717174.1"
                     /translation="MKYVLYITFASFIFACSCKQTSGRSPLPNANPEELAQSVITCLA
                     ANDTATLMKMTLTESEVNEKMSVSGFDITDSMMKENNARFAVSLREKIGSTFAMLKRE
                     GEGDSVVWKNAKTVRVEMPQEQIAGGFGLAHVFFSSGKKNAEFYFTAMRTKNGWKVAG
                     IFSY"
     gene            595..825
                     /locus_tag="VFU15_05060"
     CDS             595..825
                     /locus_tag="VFU15_05060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717175.1"
                     /translation="MGRMDVNGIFARISRCIETSGSLLQMETSERMLQLFRKQNENPE
                     LSEKLEMIFRRKAETLHYYEWKLFRDFGMEAA"
     gene            complement(882..2696)
                     /locus_tag="VFU15_05065"
     CDS             complement(882..2696)
                     /locus_tag="VFU15_05065"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HEU4717176.1"
                     /translation="MNGVRWRTWTMNKNIPSYLASVSVAPYTQVNWTHTGMYGTFPII
                     LAAVPADTTPMKNSFVHLDDAITAFENRYGPYEWPRVGYCNVPFSSGAMEHATNIAYP
                     HLAASGNLTYEANIMAHELSHHWWGDLATCHDEGEMWLNEGWATYSQYVFTEWEYGHT
                     AYSTGIRSNHDNVIHYAHHKEGGYRAVSGVPHQYTYGDHVYLKGADVAHTLRGYMGDS
                     LFWAGLHYHLQHRQYTDVTSAQFRDDLIAATGLTYLNDFFDDWVFNPGFPHFSIDSVV
                     SVPAGLNYNVTVYIKQKLVGAPNYFTNCPIDVSFIDSNWQPTVERVFVSGQYSNFSFT
                     LPFDPAMTALDMNGKISDAITDEAQTISTTGVHTFTAARCTLTVNTISDSAFVRIEHN
                     WAAPDSIRNNTNNFRISSLRYWTIDGIFPQNFEATARLWYDGRTNATSGGGYWLDNDL
                     TVTNGDSILLLYRRNAADDWHEWPHYTKTRVGTPATSKLGYVDVDSVAPGEYTFANGV
                     SSVVSVQAVAPAPAPEVYAYPNPAGDELTVQWSVTDVRNVLVNVYDSDGKLVHSVYTT
                     SDKAELETAQWNDGIYYVEVIGDRQLLGRKQVAVVHLR"
     gene            complement(2693..3322)
                     /locus_tag="VFU15_05070"
     CDS             complement(2693..3322)
                     /locus_tag="VFU15_05070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717177.1"
                     /translation="MKKIITAAAAFLPFLLFAQSANNDPVTSASSWTGSPVTQATGTI
                     SDSIDILNYNISLNITDFTTDTIRGGTTIHYAARVNNITVLPLDLLHMTIDSIVENSG
                     QLTYSYNDTLLSVNLPAAMNIGDTADMAVYYHGKPQMDPSGWGGFYFQGTCATISALV
                     SLSIRTTSAAPGSRVSIISSSVPPMFFISVPTTGKSRSATVFSPVIRQT"
     gene            3434..3769
                     /locus_tag="VFU15_05075"
     CDS             3434..3769
                     /locus_tag="VFU15_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012026213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEU4717178.1"
                     /translation="MGATKTEHFTEKQNTIATLIKALAHPARIAIVEYLMKTEACICG
                     DIVDELPLSQPTVSQHLKELKTAGLIKGNVEGNTICYCIDEKAIAKLQSYFAGISAKL
                     EKKKDNKCC"
     gene            3784..4254
                     /locus_tag="VFU15_05080"
     CDS             3784..4254
                     /locus_tag="VFU15_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018343128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6428 family protein"
                     /protein_id="HEU4717179.1"
                     /translation="MKLSELNHHLGELTQLNLVLPGGEPVPAHFHITEAGLTTKHFVD
                     CGGTVRTEKYANLQVWVAQDTDHRLSPVKLKNILDIAKNLFEGEDPEVEIEYQSQTIG
                     RYGLDFRGNSFQFTPKRTDCLAGDQCGVPAKKKVKLSELQTASSSCCTPGGGCC"
     gene            4257..4382
                     /locus_tag="VFU15_05085"
     CDS             4257..4382
                     /locus_tag="VFU15_05085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717180.1"
                     /translation="MKQKLTRYRKHIVITVSITLLQLIFGFDPKFCAINIIWLFV"
     gene            4392..4805
                     /locus_tag="VFU15_05090"
     CDS             4392..4805
                     /locus_tag="VFU15_05090"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015028195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HEU4717181.1"
                     /translation="MKKILVLCTGNSCRSQMAEGYLRFFAGNKAEVFSAGIETHGLNP
                     RAVAVMKEDGIDISAHTSDNVDKYRNIDFDFVITVCDNAKESCPVFPSQAKKFHRNFP
                     DPAKIKGTEEEIMNGFRHVRDEVKAYCLAFVNENL"
     gene            complement(4812..5309)
                     /locus_tag="VFU15_05095"
     CDS             complement(4812..5309)
                     /locus_tag="VFU15_05095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717182.1"
                     /translation="MLFLLSAYIFRGSIYRACFSYKLVRLRSEATPPYALSVTGQDIQ
                     GIAEAGLEGSSALLSFTTGPCATGVPKLQYGGKTNCIGYSNLCAGICEMKFRANPSLK
                     DARAETWVAQIFFFGWNVHRLFSDPFWKDHDVVLLTDGAAGDRILLDPTLYDYTGIGF
                     VRERK"
     gene            5442..6695
                     /locus_tag="VFU15_05100"
     CDS             5442..6695
                     /locus_tag="VFU15_05100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717183.1"
                     /translation="MKPFVSFLVASLLVFHVHAQQDSTWFPKLAHKLGFGTDPQKKYE
                     KAKQKGTLEKLSLKDRILILNFALNEVKADSSGEAKVDRVEVNEMIKDVIVTTPEKNV
                     RDLFRMMGTDVKGFRERFRFLPFEHMSLILGYVEPAFRKSEKDSQVFDIPYPAFQAQL
                     SYNIDHQGDNLFQSHYTDFCGKVALTRLWIKYNPKDYARFMTDLYYKGESTWNGMEFH
                     TPETVIHAINHNQITEDPNQKVLYKNPETTEKMPVMMDMVLYLTLASSFHTFPFNMKQ
                     YDADKHMENSAWAGAAINPEVSLLRAIGFRVEKVGDNFRGVSDAQFTQIKQDGAPGSD
                     RHVMLLVNSSMLDKISGADTAFIAPRGIEHKSLGTHWITVDYIDENTDTFSFWEYGTY
                     RKVKGIAQMQKIIAGGIIVDGYEPK"
     gene            complement(6699..>7879)
                     /locus_tag="VFU15_05105"
     CDS             complement(6699..>7879)
                     /locus_tag="VFU15_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU4717184.1"
                     /translation="RLMSELNGKEKVRVLLAQALFGNPDILLLDEPTNDLDVETIGWL
                     EDFLADYQNIVIVVSHDRHFLDAVCTHVCDIDFRKITLYTGNYTFWYQASQLAAKQRS
                     DQNRKTEDKRKELQEFIMRFSANASKSRQATSRKKLLEKLNIDELPASNRKYPAIIFK
                     MDREPGDQILNVENLSKKHEGTTLFSNVNLRLRRGDKIGFLAKDHLALTKFFDCIAGE
                     DQPDGGRIEWGQTIQTAYLPNDNSKFFSGNDNLVDWLRQFSKDKDETFIRGFLGKMLF
                     SGEETLKKVSVLSGGEKVRCMVSRMMLFNANCVILDEPTNHLDLESITAMNNSLKDFK
                     GIVLFTSHDHELMQTVANRIVEITPKGAIDKMMTYDEYINDEEVKAQRAKMNVKSAVA
                     "
BASE COUNT         1915 a         2067 c         2119 g         1778 t
ORIGIN      
        1 gtaggaaaaa atcccggcga ccttccaccc gttcttcgtt ctcatcgcgg tgaaataaaa
       61 ttcagcgttc ttttttcccg atgaaaagaa aacatgtgcg agtccgaagc cgccggcgat
      121 ctgctcctgc ggcatttcca cgcgaaccgt tttcgcattt ttccacacca cgctgtcgcc
      181 ttctccttct cttttcagca tcgcaaatgt gctgccgatc ttctcacgaa gtgaaacggc
      241 gaaccgcgcg ttgttttcct tcatcatgga gtccgtgatg tcgaaacccg acacgctcat
      301 tttttcgttc acttcgcttt ccgtgagggt cattttcatc agcgttgccg tgtcgttcgc
      361 cgcgaggcag gtgatcacgg attgcgccag ctcttccgga ttcgcgttgg gcagcggcga
      421 tctgcctgaa gtttgtttgc aggaacatgc gaaaatgaag gaggcgaacg ttatatataa
      481 aacgtatttc ataccgtatg acgttccgtt tacgccaaat gtaaagcgaa aatcacaccc
      541 cggaatactt tggcacagat tctgatttta tgctttacaa caacacagaa acgtatggga
      601 aggatggacg tcaatgggat ctttgcgcgc atcagccggt gcatagaaac aagcggttcg
      661 ctgctgcaga tggagacctc ggagcgcatg ctgcaattgt tcaggaagca gaatgaaaat
      721 cccgagctga gcgaaaagct ggaaatgatc ttccggcgga aagcggaaac gcttcactac
      781 tacgaatgga aactgttccg ggatttcggc atggaggctg cctgatcccc gggtttggtt
      841 aaacagtgca aagctcttcc ggaagggagg gctttgtttc tttaccgcag gtgcaccacc
      901 gccacctgct ttcttccgag cagttgcctg tcgccgatca cttcgacgta ataaatacca
      961 tcgttccatt gcgcggtctc aagctccgcc ttatcggatg tcgtgtacac ggaatgaaca
     1021 agttttccgt ctgaatcgta cacattcacg agcacgttgc gcacatccgt taccgaccat
     1081 tgcacggtga gctcgtcgcc cgcgggattc gggtaggcat acacttccgg cgcgggagcc
     1141 ggcgcaacgg cctgcacgct cacaacggat gaaacgccgt tcgcaaatgt gtattccccg
     1201 ggagcaacgg aatccacatc gacgtaaccg agctttgaag ttgcgggcgt acccacacgt
     1261 gtttttgtat aatgcggcca ctcgtgccag tcgtccgcgg cattacgacg gtacagcagc
     1321 aggatgctgt ctccgttcgt aacagtgaga tcattgtcga gccagtaacc gccaccggag
     1381 gtagcgttcg ttcttccgtc gtaccataaa cgcgccgtcg cttcgaaatt ctgcggaaag
     1441 atcccgtcga tggtccagta gcgcagcgag gagatgcgga aattattcgt gttgttgcga
     1501 atggaatcag gcgctgccca gttatgctcg atgcggacaa acgcagaatc cgagatcgta
     1561 ttcaccgtaa gcgtgcagcg tgcggccgtg aacgtgtgaa cgcccgttgt cgagatcgtc
     1621 tgcgcttcat cggtgatcgc atcgctgatc tttccgttca tgtcaagcgc ggtcattgcg
     1681 ggatcgaacg ggagcgtgaa cgagaaattg ctgtattgcc ccgaaacgaa aacgcgctcc
     1741 acggtcggct gccagttgct gtcgatgaat gagacgtcga tcgggcaatt cgtgaaatag
     1801 ttcggtgcac cgaccagctt ctgcttaatg taaaccgtga cgttataatt cagtccggcc
     1861 ggaacggaaa caaccgaatc gatcgaaaaa tgcgggaagc cgggattgaa cacccagtcg
     1921 tcgaagaaat cattgaggta cgtaagaccg gtcgctgcaa tgagatcgtc ccggaactgc
     1981 gcgctcgtga catccgtgta ctggcggtgc tggagatgat aatgcaatcc tgcccagaac
     2041 agcgaatcgc ccatgtaccc gcgcagcgta tgcgcgacgt cggctccttt caggtaaaca
     2101 tgatcgccgt acgtatattg gtggggaaca ccggaaaccg cgcggtaacc gccttccttg
     2161 tggtgcgcgt aatgaataac gttatcgtga ttggaacgga tacctgtgct gtaagccgtg
     2221 tgaccgtatt cccattccgt aaacacatac tgcgaatacg tcgcccagcc ttcgttgagc
     2281 cacatttcgc cctcgtcgtg gcatgtggcg agatcgcccc accagtgatg cgaaagctca
     2341 tgcgccatga tgtttgcttc gtaggtaaga tttccgctgg cggcgagatg gggataggca
     2401 atattcgtcg cgtgctccat tgcccccgag ctgaacggaa cgttgcagta accgacgcgc
     2461 ggccattcgt aaggtccgta ccggttttca aatgcggtaa tagcatcgtc aagatgcacg
     2521 aacgaatttt tcatcggcgt cgtgtcggcc ggaacggcgg caaggatgat ggggaatgtt
     2581 ccgtacatgc ccgtgtgcgt ccagttcacc tgcgtgtacg gcgcaacgga aacggaagcg
     2641 aggtaggaag gaatgttttt gttcatcgtc catgtgcgcc agcggactcc gttcacgtct
     2701 gtcgtatcac tggtgagaac accgttgcag aacgcgattt tcccgttgtc ggaaccgata
     2761 tgaaaaacat aggtggaacg ctcgacgaaa ttatcgaaac acgggaacca ggcgcggccg
     2821 aagttgtgcg gatcgacagc gaaaccaacg ccgagattgt agcgcaggtt ccctggaaat
     2881 aaaaaccacc ccagccggaa ggatccatct gcggtttgcc atggtagtaa acggccatgt
     2941 ccgctgtatc gccgatgttc atcgccgcgg gaagattcac ggacagcagc gtgtcgttgt
     3001 aggaataagt cagctgcccg ctgttctcca cgatggaatc gatcgtcatg tggagcaggt
     3061 cgagcggaag aaccgtgatg ttgttcactc tcgccgcgta atgaatggtt gtgccgccgc
     3121 ggatcgtatc ggtggtaaaa tcggtgatgt tcagcgaaat gttgtaattc aggatgtcga
     3181 tcgaatcgct gatcgtgccg gtggcctgcg tgaccgggct cccggtccag gaagacgcag
     3241 aggtgacggg atcgttattc gcagattgtg caaaaagcag gaaaggcagg aatgctgccg
     3301 cagcggtgat tatctttttc atcggtgaag aataacaatt aaagataatg caatttcctc
     3361 gtttgcagga acaacgggat caaatttgga atttccgcac cttcatcgta atattgcaat
     3421 atacttatta aacatgggcg ctaccaagac cgaacatttc accgaaaagc agaacactat
     3481 cgccacgctg atcaaggcgc tggcgcatcc cgcgcggatc gcgatcgtgg aataccttat
     3541 gaagacggaa gcctgcatct gcggggatat cgtcgacgag ctaccgcttt cgcagccgac
     3601 cgtttcgcag cacctcaaag agctgaaaac ggccgggctg atcaaaggca acgttgaggg
     3661 caatacgatc tgctactgca tcgatgagaa ggcgatcgcg aagctccaaa gctacttcgc
     3721 cgggatcagc gcgaagctgg aaaagaaaaa agacaacaaa tgttgctaac ccaaaaaaca
     3781 cagatgaaac tttctgaact caaccatcac ctcggcgaac ttacacagct gaacctcgtt
     3841 cttcccggcg gagaacctgt ccctgcgcat tttcacatca ccgaagccgg tcttaccacg
     3901 aagcatttcg tcgactgcgg cggaacagtg cgcaccgaaa aatacgcgaa cctccaggtg
     3961 tgggtcgcgc aggatacgga tcaccgcctt tcgccggtca agctgaaaaa cattcttgac
     4021 atcgcaaaaa acctgttcga aggcgaagat ccggaagtag agatcgaata ccagtcgcag
     4081 acgatcggcc gttacgggct tgacttccgc gggaacagct tccagttcac accgaagcgg
     4141 acggattgcc tggccggcga ccagtgcgga gttcccgcaa agaaaaaagt gaagctctcc
     4201 gaattgcaaa ccgcttcttc atcctgctgc actcccggag gcggctgctg ctgaccatga
     4261 aacagaagct cacccgttac aggaaacaca tcgtgatcac cgtatcgatc acgctgctgc
     4321 agctcatttt cggcttcgac ccgaaattct gcgccatcaa catcatctgg ctgttcgtct
     4381 aaattatttc catgaaaaaa atacttgtgc tgtgcacggg caacagttgc cgcagccaga
     4441 tggcagaagg ttatctccgt tttttcgccg ggaataaagc ggaagttttc agcgccggta
     4501 ttgaaacgca cggactgaac ccgcgagccg tagccgtcat gaaggaggac ggcatcgaca
     4561 tttcagcgca cacgtccgat aacgtcgaca aataccgcaa cattgatttc gatttcgtga
     4621 tcaccgtttg cgacaacgcg aaagaaagct gtcccgtttt tccttcgcag gcaaaaaaat
     4681 ttcaccgcaa ctttcctgat cctgcaaaaa taaaaggaac ggaagaagag atcatgaacg
     4741 ggttccgcca tgtgcgcgac gaagtaaaag cgtattgcct ggcgttcgta aacgaaaatc
     4801 tctgaagacg tttatttcct ttcgcggaca aaaccgatcc ccgtataatc atacaacgtc
     4861 ggatcgagaa gaatgcgatc gccggcagcc ccatccgtga ggagcacgac atcatgatct
     4921 ttccagaacg gatcgctgaa cagccggtgc acattccatc cgaaaaagaa gatctgcgcc
     4981 acccacgttt ccgcacgcgc atcttttaaa gaagggttcg cccggaattt catctcgcat
     5041 attcccgcac acaggttcga atagccgatg cagttcgttt ttccgccgta ctgcagcttg
     5101 gggacaccgg ttgcgcaggg tcccgtggtg aacgaaagca gcgcagacga tccttcgagc
     5161 ccggcttctg caatgccctg tatgtcctgg ccggtaacgg aaagcgcgta cggaggtgtg
     5221 gcttccgatc gcaggcgcac cagcttatag ctgaaacagg cgcggtaaat acttccgcgg
     5281 aaaatataag cggaaaggag gaagagcatt gaaaagacaa gcactttccg tttacgcaaa
     5341 aaagaaacca acgggcgggg gttcatcccg gtaaaggtaa agaaggcatt aggaagagat
     5401 gtccggaatt tattatgttt ggccgtcaaa aggaatcacc catgaaaccc tttgtttcct
     5461 tccttgtcgc ctccctcctg gtttttcacg tgcacgctca gcaggattcc acctggtttc
     5521 cgaagcttgc gcacaagctc ggattcggaa cggatccgca gaagaaatat gaaaaagcga
     5581 agcagaaagg cacgctggaa aagctttcgc tgaaagaccg catactcatt ctcaattttg
     5641 cgctcaatga agtgaaagcg gattcatcgg gggaagcgaa agtcgatcgc gtggaagtga
     5701 acgagatgat aaaggacgtg atcgttacca cacctgaaaa aaatgtccgc gacctgttcc
     5761 ggatgatggg cacggatgtg aaaggtttcc gggaacgttt ccgtttcctg ccgttcgagc
     5821 acatgagcct gatcctcggt tatgtcgaac ctgcgttccg gaaaagcgaa aaggattcac
     5881 aggtgttcga cattccctac cctgctttcc aggcgcagct cagctacaac atcgatcacc
     5941 agggcgataa tcttttccag tcgcattaca ccgatttctg cggaaaagtg gcgttgaccc
     6001 gtttatggat caaatacaac ccgaaagatt atgcgcgctt catgacggac ctttattaca
     6061 aaggcgaatc gacatggaac gggatggaat tccacacgcc ggaaacagtt attcacgcca
     6121 tcaaccacaa ccagatcacg gaagacccga accagaaagt gctgtacaaa aacccggaga
     6181 cgacggaaaa gatgccggtg atgatggaca tggtgcttta cctcacgctt gcttcgtcgt
     6241 tccacacgtt cccgttcaac atgaagcagt acgacgccga caagcacatg gagaacagcg
     6301 cgtgggcggg cgccgcgatc aacccggaag tttcactgct gcgcgcgatc ggcttcaggg
     6361 tggaaaaagt gggcgacaat ttccgcggtg tttcggatgc gcagttcacg cagatcaagc
     6421 aggacggcgc tcccggcagc gacaggcacg tgatgcttct cgtgaacagc agcatgctcg
     6481 ataaaatttc aggagccgat acggctttca tcgcgccgcg cgggatcgaa cacaaaagtc
     6541 tcggcacgca ctggatcacg gtcgattaca tcgacgagaa tacggacaca ttcagcttct
     6601 gggaatacgg cacgtaccgc aaagtgaaag gcatcgcgca gatgcagaag atcatcgcgg
     6661 gcggaattat cgtcgacggg tacgagccga agtaaaaatt acgctacagc gctcttcaca
     6721 ttcatctttg cccgctgcgc tttcacttct tcgtcgttga tgtactcgtc gtacgtcatc
     6781 atcttgtcga tagcgccttt cggcgtgatc tccacgatcc tgttcgcgac ggtctgcatg
     6841 agctcgtggt catgcgaggt gaacagcacg atccctttga aatccttgag cgaattgttc
     6901 atcgcggtaa tggattcgag atcgaggtga ttggtgggct catcgagaat gacgcagttc
     6961 gcattgaaca gcatcattct cgagaccatg cagcgcactt tttcgccacc ggaaagcacg
     7021 ctcactttct tcagcgtctc ttcgccggaa aaaagcattt tgccgaggaa gccgcggatg
     7081 aacgtttcgt ccttgtcttt cgagaactgc cgcagccagt cgacgaggtt gtcattgcct
     7141 gaaaagaatt tgctgttgtc attggggaga taggctgtct ggatcgtttg cccccattcg
     7201 atcctgcctc cgtccggctg gtcttcgccg gcgatgcagt cgaagaattt ggtcagcgcc
     7261 aggtggtctt tcgcaaggaa accgatcttg tcgccgcggc ggagacggag attcacattg
     7321 gaaaaaagcg tcgtgccttc gtgctttttc gaaaggtttt ccacgttgag tatctggtcg
     7381 ccgggctcgc ggtccatttt gaaaatgatg gccgggtatt tccggttgct ggccggcagc
     7441 tcgtcgatgt tcagtttttc gagcagcttt ttacggctcg tcgcctgccg tgatttcgaa
     7501 gcgttcgccg aaaagcgcat gatgaattcc tgcagctctt tccgtttgtc ctccgtcttc
     7561 cggttctggt cggagcgttg tttcgccgcc agctgtgaag cctggtacca gaatgtataa
     7621 tttcccgtat agagcgtgat cttccggaaa tcaatgtcgc atacatgcgt gcacactgca
     7681 tcgaggaagt gacggtcgtg cgaaacgacg atgacgatgt tctggtagtc ggcgagaaaa
     7741 tcttccagcc agccgatcgt ttccacgtca aggtcgttcg tcggttcgtc gaggaggagg
     7801 atgtcgggat tgccgaacag cgcctgcgcg agcagcacgc ggactttctc tttaccgttg
     7861 agctcgctca tgaggcggt
//
LOCUS       DASTSF010000176         3789 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1512706,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000176 DASTSF010000000
VERSION     DASTSF010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3789
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1512706"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..610)
                     /locus_tag="VFU15_05110"
     CDS             complement(<1..610)
                     /locus_tag="VFU15_05110"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU4717185.1"
                     /translation="MKLSVIIVNYNVEHFLEQCLHSVIKASRHTETEIFVVDNNSVDG
                     SLQMLKEKFPAVHVIANRENVGFSRANNQAMRIANGEYVLLLNPDTVVEESTFDKVIA
                     FMDAHPEAGGLGVQMIDGKGNFLPESKRGLPTPSVAFFKISGLSKLFPKSKTFGRYHL
                     GFLDKDRTHEVEILSGAFMLLRKAALDKVGLLDEDFFMYGEDI"
     gene            complement(801..1424)
                     /gene="recR"
                     /locus_tag="VFU15_05115"
     CDS             complement(801..1424)
                     /gene="recR"
                     /locus_tag="VFU15_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016662883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HEU4717186.1"
                     /translation="MTGFPSKLVEEAVEAFSRLPGVGRKTALRYVLHLLKQDDDSAQK
                     FGAIFSRLKEELKYCTTCHNISDTDVCPICANPKRDHGLLCVVEDVRDVMAIENTMQF
                     NGVYHVLGGIISPLDGIGPGDIAIDPLVERVFSGNVREIIMALSTTMEGDTTNFYIYK
                     RLAASGVPVTTIARGIAIGDELEYADEITLGKSIVNRVPYEKTIATK"
     gene            complement(1518..2012)
                     /locus_tag="VFU15_05120"
     CDS             complement(1518..2012)
                     /locus_tag="VFU15_05120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717187.1"
                     /translation="MKIILRFCLPGWLISGAAAGIFNAWSEYSGEGGYDSAGKVLSLF
                     GSYMLAGIIACFPLFITWFLFHLSLILFQLRLRYKLVILAILGPLVAVLSNLAFLKYL
                     YSVYSETENPVYLFVAGQHIREAGYLFSLYYILSLAGILVLYRKYFRKMKNLDLVWRN
                     AAEL"
     gene            2124..3602
                     /locus_tag="VFU15_05125"
     CDS             2124..3602
                     /locus_tag="VFU15_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018472841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HEU4717188.1"
                     /translation="MTAGLILTCVAVYTILLFGVTWLTSRGANNETYFVGNRSSKWYL
                     VAYGMIGASLSGVTFMSVPGDVGAKHFWYFQMVLGYVLGYVVIAFVLLPLYYRLNLTS
                     IYSYLQKRFGEASYKTGAIFFIISRVIGAAFRMFIVVNVLQVFVFGPWGIPFWVTTSV
                     FIVLILLYTYEGGVKTIVWTDTLQTTFMLVSLVLSVVLISRALHLDTGRLFSFISSHA
                     KSDIFNGNWTDGQFFPKAFFGGMFITITMTGLDQEMMQKNISCRSLKDAQKNMLTFSG
                     ILVVVNLLFLSLGVLLLEYVSRNGITGVQRSDDLFPTIALNYLGPASALFFIIGLISA
                     AYPSADGALTALTASFCIDILGFNNPEKSVDEKKKKMIRHIVHICFAVVLLLVIVVFR
                     IINDDSVLRTLFAVASYTYGPLLGLFAFGILTKRKTNESLVPAVALLSPVVCWLLSTY
                     GTKIFGGYRFGNELLVINGLITFAGLYFISTPGKISVHEEAK"
     gene            3586..>3789
                     /locus_tag="VFU15_05130"
     CDS             3586..>3789
                     /locus_tag="VFU15_05130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717189.1"
                     /translation="MKKQSNRSSSLFYTIERTLRQMRFFLQSGFSGANVPLSVDQWIV
                     LAEAGRKSGRSHRDIALHTAKDPA"
BASE COUNT          809 a         1007 c         1045 g          928 t
ORIGIN      
        1 cgatgtcttc cccgtacatg aaaaaatctt cgtcgagcag gccgaccttg tccagcgcgg
       61 ctttccgcag cagcatgaat gcgccggaaa gtatctccac ctcatgcgtc cggtctttgt
      121 cgagaaagcc gaggtgatag cgcccgaacg ttttcgattt cgggaacagc ttcgacagcc
      181 cggaaatttt aaaaaaggcg acggaaggtg tgggcagtcc gcgcttggat tccggcagga
      241 aatttccttt cccgtcgatc atctgcacgc cgagcccgcc cgcttccggg tgcgcgtcca
      301 tgaacgcgat cactttgtcg aacgtcgatt cttcaacaac ggtatcgggg ttgagcagga
      361 gaacatattc gccatttgcg atacgcatgg cctggttgtt ggcgcgggaa aaaccgacat
      421 tctcccggtt ggcgatcacg tgcaccgcgg gaaatttttc cttcagcatc tgcagcgatc
      481 cgtcgacgga gttattgtcc acgacgaaga tctcggtttc cgtatgccgc gacgctttga
      541 tcacgctgtg caggcattgc tcgaggaagt gctcgacgtt gtaattgaca atgatgacgg
      601 aaagcttcac gatctggttg taatgcgtcg gtaggttaat gtgccggttc gcgcgggaag
      661 gatcgaaacc atccggctat tggcgctttg gcacgttaaa acccggccga agttacaaac
      721 gctttttact ggatgatagt attggcggaa ggattttccg ggagaccgga gtcaaatgac
      781 gcaccagcgc agggcggcat ttatttcgtc gcaatggtct tttcgtaagg cacccggttc
      841 acaatggatt ttccgagcgt gatttcgtcc gcatactcga gctcatcgcc gatcgcgata
      901 ccgcgtgcga tggtggttac cggcacgcct gaagccgcaa ggcgcttgta aatgtagaag
      961 ttcgtggtgt cgccttccat cgttgtgctg agcgccatga tgatctcgcg cacattcccc
     1021 gaaaaaacgc gttccacgag cgggtcgatc gctatatcgc ccggcccgat cccgtcgagc
     1081 ggcgagatga tcccgcccag cacatggtac acgccgttga actgcattgt gttttcgatc
     1141 gccatcacgt cgcgcacgtc ttccaccacg cagagcagcc cgtggtcacg tttcgggttg
     1201 gcgcagatag ggcagacgtc agtgtcgctg atgttgtggc aggtggtgca gtatttcagc
     1261 tcttctttca gccgtgaaaa aatggcgccg aatttttgcg cggaatcatc atcctgcttc
     1321 agcaggtgaa gcacgtaacg cagcgcggtc ttgcgtccca cgcccgggag gcgcgagaac
     1381 gcttcgacgg cttcttcaac cagctttgac ggaaaacctg tcatgatgcg caaatttacg
     1441 tggagatgaa gctgcgccgg gtccgggagg aaagcgtttt ttcaaggtgt ttatgaacag
     1501 ttttccggcc cgggagctca cagctcggcg gcgttcctcc agacgagatc gagatttttc
     1561 attttccgga aatatttccg gtaaaggacc aggataccgg ccagtgataa aatgtaatag
     1621 agggaaaaca ggtaccctgc ttcgcggatg tgctggccgg cgacgaacaa atagacggga
     1681 ttttccgttt cggaatacac cgaatacagg tacttcagga atgcaaggtt cgagagaacg
     1741 gcaacgagag gtccgaggat cgccaggatc acaagcttgt agcggagcct cagctggaaa
     1801 agtatcagcg aaagatgaaa aaggaaccac gtgatgaaaa gcgggaagca ggcaatgatg
     1861 cctgcgagca tataacttcc gaaaagcgag agaacttttc ctgcactgtc atatccgcct
     1921 tcgccggagt attccgacca cgcattgaaa attccagctg cagctcccga tatcagccag
     1981 cccggaaggc agaacctgag gataattttc attccgcgtg acatgcgtat aaagttagcg
     2041 caaccggccc atgtggacaa agctgttgat tttaattttt tcgcacgcaa atgatgacaa
     2101 atcaatcaaa ctaacttgcg cgcatgacgg ccggacttat cctcacctgc gtggcggtgt
     2161 acacgatcct ccttttcggt gtaacgtggc tgacctcgcg cggcgcgaac aatgaaacgt
     2221 atttcgtcgg gaaccgctct tcgaaatggt atcttgttgc gtatggaatg atcggcgcat
     2281 cgctttccgg cgttacgttc atgtcggttc ccggcgatgt gggagcgaag catttctggt
     2341 atttccagat ggtgctcggg tatgtgctgg gctatgtcgt catcgcgttc gtgctgctgc
     2401 cgctgtatta ccggctcaac ctgacgtcga tctattcgta tctccagaaa agattcggcg
     2461 aggcatccta caagacgggc gccattttct tcatcatttc ccgcgtcatc ggcgcggcgt
     2521 tccgcatgtt cattgtcgtc aatgtgctgc aggttttcgt gttcggtcca tggggaattc
     2581 cgttctgggt gaccacttcc gttttcatcg tgctcatttt gctttacaca tacgaaggcg
     2641 gcgtgaaaac gatcgtctgg acggacacgc tgcagaccac gttcatgctg gtgtcgctgg
     2701 tgctgagcgt cgttctcatt tcccgtgcgc tgcacctgga taccggccgg cttttctcgt
     2761 tcatctcctc gcacgcgaaa tcggatattt tcaacggcaa ctggaccgac gggcagtttt
     2821 tcccgaaagc atttttcggc ggaatgttca tcacgatcac catgacgggg ctcgaccagg
     2881 aaatgatgca gaagaatatt tcctgccgtt cgctgaaaga tgcgcagaaa aacatgctta
     2941 cgttcagcgg gattcttgtc gtggtcaacc tgctttttct cagtctcggc gtgctgctgc
     3001 tcgaatatgt ttcccgcaac gggattaccg gcgtgcagcg ttccgacgat ctttttccga
     3061 cgatcgcgct gaattacctc gggccggctt cggcgctgtt cttcatcatc gggctgatct
     3121 cggcggctta cccgagcgcg gatggcgcgc ttactgcgct cacggcttcc ttctgcatcg
     3181 acatccttgg cttcaacaat cccgaaaaat cagtggatga aaaaaagaaa aagatgatcc
     3241 ggcacatcgt gcacatctgt tttgcggtag tccttctcct tgtgatcgtc gtgttccgca
     3301 tcattaacga cgattccgtg cttcgcacgc ttttcgccgt cgcgagctat acatacggtc
     3361 cgctgctcgg cctgttcgct ttcggcatac tgacgaaaag gaaaacgaac gaatcgctgg
     3421 ttcccgccgt ggcgctttta tccccggtcg tgtgctggct tttgtcgacg tacggaacga
     3481 aaattttcgg cgggtacagg ttcggcaacg agctgctggt catcaacggc ctgatcacat
     3541 tcgccgggtt gtattttatt tccacacccg gaaaaatttc tgtgcatgaa gaagcaaagt
     3601 aaccgctcca gctcgctttt ctatacgatc gaacgcacgc tgcggcagat gcgtttcttc
     3661 ctgcagtcgg ggttttcggg ggcgaacgtt ccgctgtccg tcgaccaatg gatcgttctc
     3721 gcggaagcgg gaaggaagag cggccggagc catcgcgaca tcgctttgca cacggcgaaa
     3781 gatccggcg
//
LOCUS       DASTSF010000177         5400 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1389830,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000177 DASTSF010000000
VERSION     DASTSF010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5400)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5400
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1389830"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="VFU15_05135"
     CDS             <1..274
                     /locus_tag="VFU15_05135"
                     /inference="COORDINATES: protein motif:HMM:NF019241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1572 family protein"
                     /protein_id="HEU4717190.1"
                     /translation="NSGYVRDRDSEFASRNVHTNVLLKMIADTRAVVAKAFDGMKEED
                     LVKKYPLETFGGERDTAYVLLTLISHLNYHLGQINYLRRVFSPSAM"
     gene            complement(323..1675)
                     /locus_tag="VFU15_05140"
     CDS             complement(323..1675)
                     /locus_tag="VFU15_05140"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HEU4717191.1"
                     /translation="MKTTITLMRNLLVILALGFSVKAAAQCQAGFTYTVSGGTVTFTN
                     TSTSNFFPVFSWNFGDNTSANSYSTQGMTHTYATAGTYYVCLTLNDSLNQSCTSTFCD
                     SVTVGVGCNNLAVNVSVSQASACSACDGGASAGVSGGTAPYNFAWSNSSSSPTQTNLC
                     VGYYSVTVTDAVGCTVTGGDYVPCPDTCHADFTAWVSGNTVNVGNMSNDTASFVQFTW
                     DFGDSYSVTATNPWSHTYATGGTYTITLVMVDSLDNCSDTISSPVTVGGPTSCGAAFS
                     MQQDSFNVLQWYAYPSVTGQWPITYLWDFGDNTSSTQQYPTHTYATSGHYTICLTITD
                     ANNCSSTYCDSSSVMRTSSAGIQYFTVVPVTTGIAQQNTNGMRMVPNPANDQVLISFN
                     EEIDGNLRITDLPGNVVMDQKVSGRNVKVDVSMLPAGCYNLTVTNGTDRWNEKIVVIH
                     "
     gene            complement(1762..2874)
                     /locus_tag="VFU15_05145"
     CDS             complement(1762..2874)
                     /locus_tag="VFU15_05145"
                     /inference="COORDINATES: protein motif:HMM:NF040138.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HEU4717192.1"
                     /translation="MKNLLFALSCFAFFAGCRKDDPARNDGTPVFYVNGTLDGQPLSL
                     KAGVNDYYMYSSCPSDTNGLYEYTGQLKQQGCTSSVCPSSLTVRINDYATHAPLPTSP
                     DSALRPGNYNFLSSQPPSYAVQFHPVLQAGDSIISCTWSFGSNATDPVHTFPSGGSYF
                     VTLTANITHNFFSGTSVASDSVYVTPPSPCMTASIATSNVDQNNGTGDTVQFTSTVTG
                     GAPPYFYSWDFGDSNTGSVSNPLHSYANPGPYAVRLSVTDNNGCSTWKELNVATPHSD
                     SSVVNFTWQQLSSASLFSAVTIEWTDASGTVYSSAGGTQPGSSSFQLISEDEYQPNEN
                     NEPTKKIHFLVTCTLFNGANSLLLQNADIVFAVSHH"
     gene            complement(2934..4460)
                     /locus_tag="VFU15_05150"
     CDS             complement(2934..4460)
                     /locus_tag="VFU15_05150"
                     /inference="COORDINATES: protein motif:HMM:NF024957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEU4717193.1"
                     /translation="MTSDDKQFDDLVRGKMDSRSFEPGPDSWRKAQKLIRKERKRRRI
                     IPVFVIFILASCLMVAGITALLLRHSSGDEKQTADTNGKNPAAFTNGNENSAHAHTNG
                     TSVTGNNSRDTNGNTRNHAAGTGNTTSGGRDADQQGNGFANGNSRLSSPGSVKDNSHS
                     GSPGGNGTSSATAHANAHTYTGHEQEQHGNAAAKSNTNAHAHRTSPPQKENTDGAPAA
                     SFTGNSSRPHDAANLNGSRDSSAYAYDNGLQHFASHGNTDTAMQRLNASQPHIDTAMN
                     RARASQPNLDSAMNHVTASQPHADSAVNNLPPPSPPNSGSASFLFAEAGVSIMPATLL
                     HTPRTSVGPVLGAGYDFHAGRKLSMQAGVRYTFTGKVSDGGVSYTSNVYSFGVQQVTS
                     GLRITRLHYLCVPVSASWHFTGRSALTGGASLNYLFTTESRLSEGNSPGKTVFGYGKN
                     LSSYDVLLSAGYGFYFSPHWSAHASVYYGLLALKNGNEYGIDLFDHNHGMTLTLQYGF
                     "
     gene            complement(4457..5068)
                     /locus_tag="VFU15_05155"
     CDS             complement(4457..5068)
                     /locus_tag="VFU15_05155"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEU4717194.1"
                     /translation="MKTWSINGLPANVPIVVEDQLLADCVRGKSRAQYRLYSLCYSFM
                     MSVCIRYTASRDDAEDLLNRSFLKILSNIEKYRKEVPFGLWVRRITINTIIDEYRKNR
                     KEKEMTELVDFDEQEVESDTAAINGFLEKTDAEQLQRLIDHLPEMSRKVLNLFVVDGY
                     GHKEISEMLNISEGTSKWHLNNARTKLKELITKFLPHFKTLAS"
     gene            complement(5164..>5400)
                     /locus_tag="VFU15_05160"
     CDS             complement(5164..>5400)
                     /locus_tag="VFU15_05160"
                     /inference="COORDINATES: protein motif:HMM:NF015298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HEU4717195.1"
                     /translation="GTLVKVTNLANDSVVVVKITDRLPKSSPRSIDLTPAAAKKLDFY
                     SRGLTKVTIGAVGNAPVNRAPQKQRKAHHPKKKC"
BASE COUNT         1130 a         1378 c         1552 g         1340 t
ORIGIN      
        1 gaattccggt tatgtacgcg accgcgacag tgaattcgct tcgcgcaacg tgcatacgaa
       61 cgtgctgctg aaaatgatcg cggacacgag agctgttgtc gcaaaagcgt ttgacggaat
      121 gaaggaagag gacctcgtga aaaaataccc gctcgaaaca ttcggcgggg agcgcgatac
      181 cgcctatgtg ctgctgacgc tcatctccca tctcaattac cacctcgggc agatcaatta
      241 cctgcggaga gttttttcac cttctgcgat gtaaaaaaaa agacccgccg cagcgggtct
      301 ttctcgtgtt tgcccaaaca catcagtgga tcaccactat tttttcattc cagcgatcgg
      361 ttccgttcgt gacggtgagg ttgtagcaac ccgcaggcag catgctcacg tccactttca
      421 cgttacgtcc gcttactttc tggtccatca ccacgttgcc cggcagatcg gtgatgcgga
      481 ggttgccgtc gatctcttca ttgaaggaaa tcagcacctg gtcgttggcc gggttcggca
      541 ccatgcgcat accattcgta ttctgttgtg cgataccggt cgttaccggc accacggtga
      601 agtattgtat tcccgcagaa gatgtgcgca tgaccgagga actgtcgcaa taggtgctgc
      661 tgcagttatt cgcgtcggtg atcgtcaggc agatcgtgta gtggcccgaa gtagcgtatg
      721 tatgcgtggg atactgctgc gtggacgatg tgttgtcgcc gaaatcccac aggtaggtaa
      781 tcggccactg gccggttacg ctcgggtatg cataccactg gagaacgttg aacgaatcct
      841 gctgcatgga gaatgcggcg ccgcaggaag taggtccgcc tacagttacc ggcgagctta
      901 tggtatccga gcaattatcc agtgagtcga ccataacgag cgtgatggtg taggttccgc
      961 cggtagcgta agtatggctc cacgggtttg ttgcggtcac cgaataggaa tcaccgaaat
     1021 cccacgtgaa ctgcacgaat gaagccgtat cgttcgacat gttgcccacg ttaaccgtat
     1081 tgccgcttac ccatgcggtg aaatctgcat ggcaggtatc cgggcaaggc acataatcgc
     1141 cgccggttac ggtgcatccc accgcatccg tcacggtaac tgaatagtag ccgacacaga
     1201 ggttcgtctg cgtgggactg gaagaagaat tcgaccaggc gaagttgtac ggtgcggttc
     1261 cgcccgaaac gccggcggaa gctccgccgt cacaggcgga acaggcggaa gcctgggaaa
     1321 cggatacgtt taccgcgagg ttgttacagc ctacgcctac tgttactgaa tcgcagaacg
     1381 tgctcgtgca gctctggttc agactgtcat tcagcgtaag gcacacatag tatgttccgg
     1441 ccgtcgcata cgtatgcgtc atcccctgtg tgctgtacga attcgcgctt gtattgtccc
     1501 cgaagttcca gctgaagacc gggaagaagt tggatgtcga cgtgttcgtg aaggtcacgg
     1561 ttcccccgct cacggtatac gtaaagccgg cctggcattg cgctgccgct ttcacagaga
     1621 aaccgagcgc gaggatcacg agcaggtttc tcatcagtgt aatagtcgtt ttcattttta
     1681 ttttttttaa ggttgggtat gattcgcttt tcagtgagat acacgggtaa gacgtgctga
     1741 aggttgggtt atcttataaa attaatgatg tgaaacggca aaaacaatat ccgcattctg
     1801 cagcaacagg gaattggcgc cgttgaacag tgtgcacgtc acgagaaagt ggatcttttt
     1861 cgtgggctcg ttgttctcat tcggctggta ttcgtcttcg gaaatgagct ggaacgaaga
     1921 agaaccgggt tgtgttccgc ctgcggaaga atagaccgtt ccggatgcgt ctgtccattc
     1981 gatcgtcact gctgaaaaga gcgaagcgga agaaagctgc tgccacgtga aattcaccac
     2041 cgacgaatcg gaatgcggcg tggcgacgtt cagttctttc cacgtgctgc agccgttgtt
     2101 gtccgttacg gagagccgga cggcgtaagg ccccggattt gcatagctgt gcagcgggtt
     2161 gctgaccgac ccggtattgc tgtcgccgaa atcccaggag taaaaatagg gcggagctcc
     2221 accggtcact gttgaagtga attgtacggt atcgccggtg ccgttgttct ggtcgacatt
     2281 gctcgtggcg attgatgcgg tcatgcaggg tgaaggtggc gtgacataaa cggaatcgga
     2341 ggcgacgctc gtaccggaaa agaaattgtg cgtgatgttg gcggtcagcg tcacgaaata
     2401 gctgccgccc gaagggaacg tatgcaccgg gtcggttgca ttgctgccga acgaccaggt
     2461 gcagctgatg atcgaatcgc cggcctgcag cacaggatgg aactgcacag cgtaagacgg
     2521 cggctgcgaa gacaggaaat tgtagttccc cggccggagc gcgctgtcgg gcgatgtggg
     2581 cagtggagcg tgtgtcgcgt aatcgttgat gcgcacggtc aggctcgacg gacaaacgga
     2641 ggacgtgcat ccctgctgct tcagctggcc ggtgtattcg taaagcccgt tcgtgtccga
     2701 aggacaggag ctgtacatgt agtaatcgtt cactcccgct ttcagcgaca acggttgccc
     2761 gtccagcgtt ccatttacgt aaaacaccgg cgttccgtca ttccgggcgg gatcgtcttt
     2821 ccggcatccc gcgaaaaaag cgaagcacga aagagcgaac agcaggttct tcatgctgtg
     2881 ccgcgaagca gttctgagaa tattatggtt atgcctgtcc atcgttatgc ctgttagaaa
     2941 ccatattgca gggtgagcgt catgccgtga ttgtgatcga aaagatcgat gccgtattcg
     3001 ttcccgttct tcagcgcgag cagcccgtaa tacaccgagg catgcgcgct ccagtgcggt
     3061 gaaaaataaa atccgtaacc ggcggaaaga agaacatcgt acgacgaaag attttttcca
     3121 tagccgaaaa cggtttttcc cggggagttt ccttctgaaa gccggctttc cgttgtgaaa
     3181 agataattca gcgaagctcc gccggtcagt gcggatcgtc cggtgaaatg ccagctggct
     3241 gaaacgggca cgcacaaata atgcaggcgc gtgatgcgca gcccgctggt cacctgctgc
     3301 acaccgaagc tgtatacatt cgatgtgtac gatacgccgc cgtcgctcac tttcccggtg
     3361 aaagtatagc gtacgccggc ctgcatgctc agcttccgtc ccgcgtggaa atcatacccg
     3421 gcaccgagca cgggtcccac cgatgtgcgt ggcgtatgca gcagcgtggc gggcatgatg
     3481 ctcactcccg cttccgcgaa aaggaagctg gcgctgccgc tgttgggcgg ggaaggaggt
     3541 gggaggttgt tcaccgcgga atcggcatgt ggctgcgatg cggtcacatg gttcatcgca
     3601 gaatcgagat tgggctgcga cgctctcgcc cggttcatcg cagtgtcgat gtgtggctgc
     3661 gatgcgttca gccgctgcat cgcggtatcg gtgttcccgt gcgatgcgaa atgctgcagg
     3721 ccgttatcgt aagcataggc ggaagaatcc ctgctcccgt tcaggttcgc tgcgtcatgc
     3781 ggacgggaag aattccctgt gaaagaagct gcaggagctc catccgtgtt ttctttttgc
     3841 ggaggagacg tgcggtgtgc atgagcattc gtgttgcttt tggcagcagc atttccgtgt
     3901 tgttcctgtt catgaccggt gtatgtgtgt gcgtttgcat gcgctgtggc ggaagatgtt
     3961 ccgttaccgc ccggcgagcc ggaatgactg ttgtctttca cgctgccggg tgaagacagc
     4021 cgggaattcc cattggcgaa accatttccc tgctgatcgg catcacgacc gccggatgtc
     4081 gtgttcccgg ttcccgccgc atggtttcgg gtgttcccgt tggtgtcccg ggaattattt
     4141 cccgtaacag aggttccgtt cgtatgtgca tgagcgctgt tttcgtttcc gttcgtaaat
     4201 gccgccggat tttttccgtt cgtatccgct gtttgttttt catcgccgga agaatggcgg
     4261 agcagcagtg ccgtaatgcc cgcaaccata aggcaggaag cgaggatgaa gatgacgaag
     4321 acaggaatga tcctccttct tttccgttct ttccggatca gcttctgcgc ttttctccag
     4381 ctgtccggac ccggttcgaa cgaacgtgaa tccatcttgc ctctcaccag gtcgtcaaat
     4441 tgtttgtcgt cggatgtcat gatgcgagtg ttttgaaatg cgggagaaat ttggtgatca
     4501 gctctttcag ctttgtgcgg gcattattaa ggtgccattt ggacgtgcct tcggaaatgt
     4561 tcagcatttc ggagatctct ttgtgcccgt atccgtcgac cacgaaaagg ttgagcactt
     4621 tccggctcat ttccgggaga tgatcgataa gccgctgcaa ttgttccgcg tccgttttct
     4681 caagaaaacc attaatcgcc gcggtgtcgc tttccacttc ctgttcatcg aaatccacca
     4741 gctctgtcat ttctttttcc ttcctgtttt tccggtattc gtcgatgatc gtattaatgg
     4801 tgatgcgtct tacccatagt ccgaacggca cttctttccg gtacttttca atattgctga
     4861 gtattttgag aaagctccgg ttaagcaggt cttctgcgtc gtccctggaa gctgtataac
     4921 ggatgcaaac ggacatcatg aatgaatagc acaggctgta gagccggtac tgcgcgcggc
     4981 ttttacctcg cacgcaatca gccagtaatt gatcttcaac tacgatcggc acatttgcag
     5041 gtagcccgtt tattgaccag gttttcattc cgatacacgt gaaaattgtg ctccgggttg
     5101 ggtgggagaa taaaaatgtt gccggaagtg caaaggtacg gtcgcggaaa gcctttttgc
     5161 cggtcagcac ttttttttcg ggtgatgtgc cttcctctgt ttttgcggcg ccctgttcac
     5221 cggcgcgttt cccaccgccc cgatcgtcac tttcgtaagt ccccggctgt agaaatccag
     5281 tttttttgcc gcggccggcg tcaggtcgat gctgcgcggc gaacttttgg gaagacggtc
     5341 ggtgatcttt acgaccacca cggaatcatt ggcgaggttc gtcactttca ccagcgttcc
//
LOCUS       DASTSF010000178         5987 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_50232,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000178 DASTSF010000000
VERSION     DASTSF010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5987
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50232"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1494
                     /gene="dacB"
                     /locus_tag="VFU15_05165"
     CDS             <1..1494
                     /gene="dacB"
                     /locus_tag="VFU15_05165"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HEU4717196.1"
                     /translation="VFMTGGDKAPSFIVKKEKDSMPAKEVVQADTTALGRLKAWVDSL
                     NNAPEMDGGGWSFYLADADSGKPICAVDINRGYVPASTMKVVTTGTALSLLGPGFRFA
                     TLLQYDGKIDEATRTLHGNIYIHGGGDPSLGSETFGSSIGKVVSAWSEAIKKLGIDSI
                     DGSIIGDAESFDRDPVPGGWSWTDMQSDYGTGPCGLNIHENLYTLQITPSGTGTVIHT
                     DPYIPGLKLYNQVIHNPGVGKSYAYVMGAPFQFERTAIGEVSGYHEEMSAIPDPALYC
                     AQVLRSSLSANGIVVRDSATTLRLMKLNHQKPDAAEGRKLITTMYSPSLSELVFHTNQ
                     VSQNFYAESILRAISWKQNGYGSTVSSVGIVYQFWRSHQLDLRGLCMVDGCGLSRMNT
                     ITTHQLVEMLRVYAKDSAVFHSFYHSLPVAGESGTIRKLADSTAADGNLHAKSGTMTR
                     VKSYAGYVRTRSGRLLTFAMISNNTLWDVVDWKYKFEKIFVLMAELP"
     gene            1609..2211
                     /locus_tag="VFU15_05170"
     CDS             1609..2211
                     /locus_tag="VFU15_05170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717197.1"
                     /translation="MAPANEKHPGDKLIAGVKKSATELENLQVQIALGEMEARDKFEE
                     IKKKFNRTISEAKLKTKEGGKKAQEVQAYLEDLQVQLALGKAETAEAFEEQKKKIFRA
                     VNKLDKMLEVNPWAAKLDEQVLHELEKFRIKMEMLGTRLSLGKERVKAKLNEEKIDVK
                     ERVAKIRTKMMLRKNAASIKWKHRKEEVKEAYKHLKKAFV"
     gene            2339..3184
                     /locus_tag="VFU15_05175"
     CDS             2339..3184
                     /locus_tag="VFU15_05175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717198.1"
                     /translation="MKKLLILLLVVPMWYSCKNDTPEKDPVKDSLVNETQNLNGLNAK
                     QAAALDSFFRAMNDIQGNLDEIKKKEKIISKDTASGDVNGRKEQITNDIQSIYDLMIK
                     NKQRLAAAKKQLKQSDLNIASMQQTIDNLTTQLAEKENEITDLKDQLSKLDLQLSNLS
                     MNYTELQQESDAKTDALNEAWYAFGTSKVLTQQGVLTKEGGFIGIGKSQKLAADMNTN
                     YFTKIDITQTKEIILGAKKAKLVTTHPAGSYEIQGDNGRADKLVITDPDKFWSVSKYC
                     VIVVE"
     gene            3245..4492
                     /locus_tag="VFU15_05180"
     CDS             3245..4492
                     /locus_tag="VFU15_05180"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEU4717199.1"
                     /translation="MKRFFLLPLFALLFFSCANRKVENVQPPAVDSTPIIYRQINADL
                     KKQQLDTFFSNRFVDGRFSGCVLIAQRGIILFEKAFGWANHEKRDSLHLDNSFQLASV
                     SKQFTASAVMLLHQEGKLQYDDTLGKYFPHTPWHGLTIRELLTHRCGLDHYTNVVDNY
                     FISHDTTPSVFSNDSAISMMEDLSPHFVRAPGTKFNYSNTGYMLLAGVVEKISGQPFW
                     EFMQQNFFAPLGMQHTWIATDGQEHPGKTRGYFSKWIWWQDNYLDNVTGDKGVYSSVE
                     DMYKWDRSLKNNTILKKEVLDEAYKGYSPELEARKPWNYGFGWRILKFDDGAMAVFHN
                     GWWHGYTSAFYRGLSDDVTVVILCNKYNRGIYDVRPILQILGAHHQPVDVEENEGDSA
                     ADSPHKSVPAKKSAPEKKRSRKK"
     gene            4489..5118
                     /locus_tag="VFU15_05185"
     CDS             4489..5118
                     /locus_tag="VFU15_05185"
                     /inference="COORDINATES: protein motif:HMM:NF015892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU4717200.1"
                     /translation="MNKHILAVEKTARYFTLGDVASAKNILFALHGYAQSAGEFLAQF
                     EPVMQEGLVIVAPEGLHRFYARGGAGNVVASWMTKEERLDDIRDYVAFLDKVYRQIKQ
                     AAPDAEMTALGFSQGAATASRWIAAGNSIVKELILFCGFFPPDVPPGKISGDVKITVV
                     TASNDKFISAEEEKKQLAEMKKIYPSLHHIRFDGEHTIDVATLEKILGK"
     gene            complement(5160..>5987)
                     /locus_tag="VFU15_05190"
     CDS             complement(5160..>5987)
                     /locus_tag="VFU15_05190"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013634325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HEU4717201.1"
                     /translation="PLSVELIIVATATPEHQFPATANIVSDMIGAKNAWSFDLMGACS
                     GFLYALNVGRQFIESGAHKKVLVIGSDKMSTIVDYTDRRTCILFGDGAGCALLEPDTT
                     GHGILDAHMYSDGAGKPNLFQLAGGSFRPASEETVRLRQHYISMNGQGVFKLAVEKMA
                     DAAEEMMRRNHLTADDIAFLVPHQANKRIIDATANRMGIGSEKVMLNIEKYGNTTTAT
                     LPICLTEWEKQLKTGDNIILATFGAGFTWGGMYVKWAYDGAKAAARRTEEEKAAMPV"
BASE COUNT         1508 a         1590 c         1619 g         1270 t
ORIGIN      
        1 gttttcatga cgggtggtga taaagcaccc tccttcattg tgaagaagga aaaagattca
       61 atgccggcga aagaagtggt gcaggccgac acaaccgccc ttggccgcct gaaggcctgg
      121 gtcgattcgc tgaataacgc cccggaaatg gacggcggcg ggtggagctt ttacctggcc
      181 gacgccgaca gcggcaaacc catctgcgca gtcgacatca accgcgggta tgtgccggcg
      241 tccacgatga aagtcgttac cacgggaacg gcgctttcgc tgctcggacc cggtttccgt
      301 tttgccacgc ttttacaata cgacgggaag atcgatgagg ccaccagaac gctgcacggg
      361 aatatttaca tccacggcgg cggcgatcct tccctcggct cggaaacgtt cggcagcagc
      421 atcggaaagg tcgtttccgc ctggtcggaa gcgataaaaa aactcggcat cgacagtata
      481 gacggaagca ttatcggcga cgcggaatcg ttcgaccgcg atcctgttcc cggcggctgg
      541 tcgtggacgg atatgcagag cgattacgga accggtccct gcggactcaa tatccacgaa
      601 aacctgtaca cgctccagat cacgccttcc ggcaccggca ccgtcatcca caccgatccg
      661 tacattcccg ggctgaagct ctacaaccag gtcattcaca atcccggcgt cgggaaatca
      721 tacgcctacg tcatgggtgc gccgttccag ttcgaacgca ctgcgatcgg tgaggtgagc
      781 ggttaccacg aagagatgag cgccattccc gatccggcgt tgtattgtgc gcaggtgctg
      841 cggtcttcgc tctctgcaaa tggaattgtt gtccgcgatt cggcgacgac gcttcgactc
      901 atgaagctga accaccagaa accggatgca gcggaaggcc gcaagctgat cacgacgatg
      961 tattctccgt cgctgtcgga gctcgttttt catacgaacc aggtgagcca gaatttttat
     1021 gcggaaagca ttctccgcgc catttcctgg aagcagaacg gctacggttc caccgtcagc
     1081 tcggtcggca tcgtttacca gttctggcgc agccaccagc tcgacctgcg cgggctctgc
     1141 atggtggacg ggtgcggtct ctcgcgcatg aacacgatca ccacgcacca gctggtggaa
     1201 atgctgcgtg tctacgcgaa ggattcagcg gttttccatt cgttctatca ttcgcttcct
     1261 gttgccggtg aatcgggaac gatacgcaag ctcgccgaca gcaccgccgc cgacgggaac
     1321 ctgcatgcga aaagcggcac catgacgcgc gtgaaatcgt atgccggcta tgtgcgtacg
     1381 cgttccggaa ggctgctcac cttcgccatg atctcgaaca atacgctctg ggatgtcgtc
     1441 gactggaaat acaagtttga gaagatcttc gttctgatgg ccgaattgcc ctgaaggctg
     1501 cgctcccttc tgcgttccat ctgacaaccg tcaatactct gcttgagggg tgtcatttcg
     1561 gtagactgaa ggttgacaga catttgctgc aaaccaaaaa acaaaaaaat ggcacccgct
     1621 aacgaaaaac atcccgggga caaactgatc gccggggtaa aaaaatcagc aacggaactg
     1681 gaaaacctgc aggtacagat cgctctcggc gaaatggaag cccgcgacaa gttcgaagaa
     1741 ataaagaaaa agttcaaccg cacgataagc gaagcgaagc ttaaaacaaa agaaggcgga
     1801 aagaaagcac aggaagtgca ggcttacctt gaagacctcc aggtgcagct ggcattggga
     1861 aaagcggaaa ccgcagaggc gttcgaagag cagaaaaaga aaattttccg cgcggtgaac
     1921 aagctcgaca aaatgctcga ggtgaatccc tgggcggcga agctcgatga gcaggtactg
     1981 catgagctgg aaaaattccg catcaagatg gagatgctcg gcacccgcct gagccttggt
     2041 aaagagcggg tcaaagcgaa gctgaacgag gagaagatcg acgtcaagga gcgcgtagcg
     2101 aagatccgta ccaaaatgat gctgcgtaag aatgccgcat cgatcaaatg gaaacacagg
     2161 aaggaagagg tgaaagaggc ctacaagcat ctcaaaaagg cgttcgtctg accagcaaag
     2221 ccccgggttt ccggggcttt cggttaagga attttaagaa aaaccggtcc aacagaaaat
     2281 cgaaaaaaaa taaacggcga atctgctttg tttacatttg ccgtgtaaac ctaccgctat
     2341 gaaaaagcta ttgatcctcc ttcttgtcgt cccgatgtgg tactcatgca agaacgatac
     2401 gccggaaaaa gatccggtga aagacagtct cgtcaatgaa acgcagaacc tgaacgggct
     2461 caacgccaag caggcagctg cgctcgattc gttcttccgt gccatgaacg atatacaggg
     2521 caatctcgat gagatcaaga agaaagaaaa gattatttcg aaagacaccg caagcggcga
     2581 cgtgaatggc cgcaaagagc agatcacgaa cgatatccag tctatctatg atctgatgat
     2641 caagaacaag cagcggcttg cagcggctaa aaaacagctc aagcagtctg atctgaatat
     2701 cgcttcaatg cagcaaacca tcgataacct gacgacgcag ctggccgaga aggaaaatga
     2761 gatcaccgat ctcaaggacc agctgtcaaa gctcgatctc cagctcagca acctgagcat
     2821 gaattacacc gagctgcagc aggagtccga tgcgaaaacc gatgcgctca atgaagcatg
     2881 gtatgcattc ggaacgtcca aagtgctgac gcagcagggc gttcttacga aagaaggtgg
     2941 tttcatcggc atcggcaaat cgcagaagct ggcggccgac atgaacacga attatttcac
     3001 aaagattgac atcacgcaga cgaaagagat cattctcggc gcgaaaaaag cgaagctcgt
     3061 aaccacgcat cccgccggtt cctatgagat ccagggtgac aacggtcgcg ccgacaagct
     3121 cgtgatcacc gacccggata aattctggag cgtttcgaaa tattgtgtaa ttgttgtgga
     3181 ataataagtc ggccgttaac agttttccgt gtaaatcctc cgcacttttg cgggggattt
     3241 ttttatgaaa cgatttttcc tgctgccgct tttcgccctt ttgttctttt cctgcgccaa
     3301 ccgcaaagtg gaaaatgtgc agcctcctgc cgtcgacagc acgcccatca tttaccgcca
     3361 gatcaacgcc gatctgaaaa agcagcagct cgacacgttc ttctcgaacc gttttgtcga
     3421 cggccgtttt tccggctgcg tgctcattgc ccagcgaggg ataattcttt ttgaaaaagc
     3481 gttcggctgg gccaaccacg aaaaacgcga cagcctgcat ctcgataatt ctttccagct
     3541 ggcttccgtt tcgaagcagt tcacggcctc tgccgtcatg ctcctgcacc aggaaggaaa
     3601 gctgcagtac gacgacacgc tcgggaaata ttttccgcac acgccctggc acggacttac
     3661 catccgcgag ctgctcacac accgctgcgg gctcgatcat tacacgaacg tcgtcgacaa
     3721 ttatttcatc tcgcacgata caacaccctc ggtcttttca aacgacagcg ccatctccat
     3781 gatggaagat ctgtcaccgc acttcgtgcg tgcgccggga acgaaattca attacagcaa
     3841 tacgggctat atgctgctgg ccggcgtggt cgaaaaaatt tccggtcagc ctttctggga
     3901 attcatgcag cagaattttt ttgcgccgct cggcatgcag catacatgga tcgccaccga
     3961 cgggcaggag cacccgggga agacgcgggg ctacttttca aaatggatct ggtggcagga
     4021 taattacctc gataacgtta ccggcgacaa aggtgtttat tcttcggtgg aggacatgta
     4081 taaatgggac cgttcactga agaacaacac cattctgaaa aaagaggtcc tcgacgaagc
     4141 gtacaaagga tacagccccg agctggaggc gcgcaagccg tggaattacg ggttcggatg
     4201 gagaatactg aaattcgatg acggggcgat ggccgttttt cataacgggt ggtggcatgg
     4261 ctacacgtcc gctttctacc gcggcctcag cgacgacgtc accgttgtca ttctctgcaa
     4321 caaatacaac cgcggaattt acgacgtgcg ccccatcctg cagatcctcg gtgcgcatca
     4381 ccagccggtc gatgtggagg aaaatgaagg tgattccgca gccgattctc cgcataaatc
     4441 cgttccggcg aaaaaatctg caccggaaaa gaaacgttcg cgcaaaaaat gaacaagcac
     4501 attcttgccg tcgaaaaaac ggcgcgctac tttacactgg gtgatgtcgc ttccgcgaaa
     4561 aacatcctgt tcgccctcca cgggtacgcg caaagtgccg gtgagtttct tgcgcagttc
     4621 gaaccggtta tgcaggaagg attggtgatc gttgcgccgg aagggctgca tcgtttttat
     4681 gcgagaggcg gtgcgggaaa tgtcgtcgct tcctggatga cgaaagaaga gcgccttgac
     4741 gacatccgtg attacgtcgc gttcctcgat aaagtgtacc gtcaaataaa gcaggctgca
     4801 cccgatgcag agatgaccgc gctcggattt tcgcagggag ctgcgactgc gtcgcgctgg
     4861 atcgcggccg ggaattcgat agtaaaagag ctgatccttt tctgcggatt ttttccgcct
     4921 gatgttccgc ctggaaaaat cagcggcgac gtgaagataa cggtcgtgac ggcctcgaat
     4981 gataaattca tttcagcgga agaagaaaaa aaacagctgg ctgaaatgaa aaagatttac
     5041 ccctccctgc atcacatccg ttttgacggt gaacacacga tcgacgtggc aacgctggaa
     5101 aaaattctcg ggaaataact tcgtgtcttt gcggacacga agaattctga acgccctgtt
     5161 tatacaggca tcgccgcttt ttcctcttcc gtccttctgg ccgcggcttt cgccccgtcg
     5221 tacgcccatt tcacgtacat gccgccccag gtaaacccgg cgccgaacgt tgcaagaatg
     5281 atattgtcgc cggtcttcag ctgcttttcc cattccgtga ggcagatggg aagcgtggcc
     5341 gttgtggtgt tgccgtattt ctcaatgttc agcatcacct tttcgctgcc gatgcccatg
     5401 cggttggccg tcgcgtcgat gatgcgtttg ttcgcctggt ggggtacgag gaatgcgata
     5461 tcgtcggcgg taagatgatt gcgcctcatc atttcttcag cggcatccgc cattttttcc
     5521 acggccagct tgaaaacacc ctgcccgttc atcgagatgt aatgctggcg gagccggacg
     5581 gtttcttccg atgccggacg aaagcttccg cctgcgagct ggaacaggtt cggctttcct
     5641 gcaccgtcgg aatacatgtg cgcatcgaga atgccatggc ctgtcgtatc gggctcgagc
     5701 aacgcgcagc cggcgccatc gccgaaaaga atgcatgtgc ggcggtcggt gtaatccacg
     5761 atggtcgaca ttttatcgga gccgatgacg agcacttttt tgtgcgcgcc gctttcgatg
     5821 aattgtcttc cgacgttcag tgcatagagg aaaccggaac aagcgcccat gaggtcgaag
     5881 ctccaggcgt tctttgcgcc gatcatgtcg gaaacgatgt tcgccgtggc gggaaactgg
     5941 tgctccggtg ttgctgtcgc gacaatgatg agctcgaccg acagagg
//
LOCUS       DASTSF010000179        16569 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_53904,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000179 DASTSF010000000
VERSION     DASTSF010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 16569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16569
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53904"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1167
                     /locus_tag="VFU15_05195"
     CDS             <1..1167
                     /locus_tag="VFU15_05195"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEU4717202.1"
                     /translation="VSFSLNMMGGNTFAQHGHTGKGVVIAILDAGFSGADSHEAFRQL
                     GLNGQIRATYDFVGKTSDVYHCKSDHGTEVFSCIAGKMGGKNMGFAPDATFLLARIMK
                     EYGNEFRGEENFVAGVEWSDKNGATLINSSAGPNDFEYMPEEMDGKTALISRAANLAA
                     RKGMLVIAAAGNEGGTRDENLLPPADSDSVLAVTAVNDTGFIADYSSVGPVFDFRTRK
                     PDVCAPGTAIVAYGDNEYGIGDGTSFSCPFVVGFAACLVEMYPDITPLGLMDTLRHSG
                     SLYPYFDYAHGNGIPQASYFFDPPGAMPETFSFGTHDSYTDIIIDDNFMPDCCRKMPT
                     LLFYNLETASGKIYRYGVIEVRSKKPLSIDNLQDVTPGTKLNVSYKGYYASKVF"
     gene            1148..1891
                     /locus_tag="VFU15_05200"
     CDS             1148..1891
                     /locus_tag="VFU15_05200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717203.1"
                     /translation="MRRKFSDIALIFIAVFVFSTGKTEAQRVIYENSTDTTAAIPKVG
                     PNRLFFFFPTYYLCTESFPVSYGYPAAFWSAGAGIDLNFKLKLWSWESLVWSAGLRHD
                     HFGISQKQVKLPPLSPDRHKREKIVTDNFTFSFSDRINFGKRGNVIGAYAEFGVTGSC
                     VFRTSYVYVDQYFDSNGPGGRQNKVKIKSTGLAYLEPFTYGPVFRIGMEYYSISLQYR
                     YNNLIRSAYSPNGQDLPKLMLSFEYSRAY"
     gene            2127..3926
                     /locus_tag="VFU15_05205"
     CDS             2127..3926
                     /locus_tag="VFU15_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013663353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEU4717204.1"
                     /translation="MEPTRVFDLLDFTPSHFGNKADFITAKENGKWVNYDTETIIRYC
                     HEMASGFIGHGLQRGERVAILANNRPEWNITDFAVQLSGAVLVPVYPTISEHDLNFIL
                     KDCEARFVFVSNNDIFLKTKTACAGLADPPRIFCFDEVEGGTSWKEVMDTGKKNPRED
                     VIGEVKKNTKPGDMFTILYTSGTTGTPKGVMLSHRNLVSNFTSLCDLPPVDETSRVLS
                     FLPLNHIYERMLVYLYFYKGPSIYYAESLEKIAENIREVKPHMFTCVPHLVQKVYNGI
                     TAKGAEQKGIKRWLFFTAVEMAEKFEIRPGGNSFFYRLKMAILGRFVFSKWRAGLGGN
                     LRVMVSGGAALEPKLARIFWAAGLPVLEGYGLTETSPVIAVNNFGPDNVRFGTVGPVI
                     KGVEVKFAEDGEILVKGPNVMLGYYKRPDATAEVIDKDGYFHTGDIGEMMEGKFLRIT
                     DRKKEIFKTSGGKYIAPQLIENLLRTSRFVEQAMVIGENRKFPAALIVPSFTFLRAYA
                     KYKGIQTGMSNEELISNETIRKRIEASVEKLNAELAQYEKVKKIILIPSEWTVNSAEL
                     TPKLSLRRKVILKNNEAAIEKMYEEADRVSEKK"
     gene            complement(3910..5376)
                     /locus_tag="VFU15_05210"
     CDS             complement(3910..5376)
                     /locus_tag="VFU15_05210"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEU4717205.1"
                     /translation="MRRFTFLFYLLLFSVCTGAQQNLVPNPSFEEPGAPVIQKFNWGR
                     YKDWKRDSLDYRTKNYLCKDWFQVTDGTPDYLNSDQSWLLGWKTKTARTGDGRMAIIG
                     GLAKNSLVSWLLYSDTYAEYVECRLLQPLEAGKTYRVEYYVALDRKSNFACHAFGAAI
                     TKDCVTVSNYHACMWGYDPYAQVINSNDHYITSDEGWVMVCDTFVAKGGERFLTLGTF
                     AGDFPKRVHSVKRSQHGGLRVAPFNKYSYYYVDDVSLTEVLPGQPVCMTPRDTVPRDN
                     VVFLIDVSGSMQQKGLIDEAKRSILPFVNTLSVNDHVTILAFSGNVQVLCEQVPASDT
                     ATIRDALDHLSAGGATNAKGAFDAAYASVRKKMIPGGTNKIVVLTDGQIYLDKKEKKK
                     IRAAVKDEGITVSVVFFGEKVPEQVQKFAESAGGTAANATGGTTQNALQNVVPAKIDD
                     TPYGQRNAGKIFTYELLTKALLPGLLALLLFGKFIFSH"
     gene            complement(5525..6907)
                     /locus_tag="VFU15_05215"
     CDS             complement(5525..6907)
                     /locus_tag="VFU15_05215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717206.1"
                     /translation="MRKLLPLLFLAFLASCISDDKDDKKDDTVAQQQPPKAPAVKPGT
                     ITDLSVRSQPANSFSVYLPSDFSYDKQWPVVIFFDAHGDGHEPLDKYKSIADELGFIF
                     VASNSSRNGIQMGEAASIGNGLIDEVKSLYPCADNEIILCGFSGGARVVSMLGGSPSD
                     LKAIICNSAAPAAPFRDKIFVGLAGLGDMNYLEMKKFNDSYPANANPHELLVFDGKHE
                     WAPVSVMRTALLITNCYKKDKSIHPVDSTNAMNALSEALISQSDSMKSMSCLLAKNLL
                     ETGTHIVTSSPGKENISKKFAAISKDPCVRSDETGWKNAEAEETVLQQELGEAVRDKD
                     TTWWRANADSYFETKSTGAEKFMRQRLRGYTSLMLYTYSNQAMKVSNLHAAESLITIY
                     SIVDPENSEWAYMKAALYMSLGLDKYVVPNLEKAIDLGFNDRLRLQNDPVFNYLHSDP
                     GFNGLFARMK"
     gene            7050..10058
                     /locus_tag="VFU15_05220"
     CDS             7050..10058
                     /locus_tag="VFU15_05220"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002705607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HEU4717207.1"
                     /translation="MKFRCVFAFFVIASAWLSCIPYPDEHSPKVYFRWDVPEPKFDFA
                     KYVDPLIGTGGHGHTFPGACAPFGMVQLSPDTRVDGSWDGCSGYHYDDDTIYGFSHTH
                     LSGTGCSDYGDILFMPENEVIFNTGADGKPGYRSSFSHRNEKAEAGYYSVVLDNTKTK
                     AELTASPRVGVHRYTFSGDNENWMLLDLTHRDEVLDSKVEIVNKHTIQGFRRSKAWAE
                     DQCVYFYAEFSEDFDEYRVGGQKGYQPEVSVVKGHKVKIAFSFGGTKNLVLKVALSPV
                     SCENAKLNMQAEVPGFDFDAVHKKVVQQWNNELGKIEVKSGDETKKKIFYTALYHCFI
                     APNLYCDVNGDYRGMDDQVHSDTVHPQYTVFSLWDTYRALHPLFTIVQPQRDQDFIAT
                     FVKQAQEGGRLPVWELSANETNCMIGYHSVPVIVDAWMKGLTNFDTATAYEAMKDAAM
                     RDERGLASYKKFGFVPAEGESESVSKTLEYAYDDWCIAQAAKRMKRDDDYAYFMHRSQ
                     SWRNVYDPQTGFMRARLNNSFVSPFDPGEVNFHYTEANAWQYSLAVQQDIPGLIAAHG
                     GDEKFIAFLDQLFSASSQTSGRKQADISGMIGQYAQGNEPSHHMAYLYDYTSQPWKTQ
                     QLVSQICNEMYTDKPDGLCGNEDCGQMSAWYVMSMMGLYDVTPGTNEYAITRPGFDQV
                     EIHLESGKNFEINDLTNDTFQLKFPEGFKPGYVFANVNGNAIHKNEISFSDMANGGKF
                     DFIRTGTNWQQVNICEDNTTIPLPPRVKSASEGPVTPMPLRTFVNRLDVTYENIPGLR
                     YFQSVNNGPYVETGTHFTINGSGIIRAFTVNETGRHSDTVMFRYMKIPGGRSITLNTA
                     YANQYAAGGNKALIDGIRGAENFRTGDWQGYEGDDVDAVVDLGSVKNVAHISIGCLQD
                     QASWIFMPQEVDFYVSDDGRNFTQAGSMMNTIDEHHDGPVLHEFYCDPGMPARYVKVV
                     AKNRGACPAWHPGKGNPAWIFADEIEIETR"
     gene            10139..11137
                     /locus_tag="VFU15_05225"
     CDS             10139..11137
                     /locus_tag="VFU15_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007094544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase"
                     /protein_id="HEU4717208.1"
                     /translation="MNRKIFLQRTALFTAATSMGVSPFSKDDIRRKEKKNPNSSPGFP
                     VVISTWNHGMPANEEAWKTLSSGGSALDAAEKGVMVVESDKNSTSVGLGGFPDRDGYV
                     TLDASIMRDDGMAGSASFVTGIEHPISLARMIMERTPHVMLVGSGAEQFAREQGITQL
                     PNKLSPAAEKAWKDWLKEKNYKPVINVENHDTIGLLVLDKDGKFAGSCTTSGLAWKLH
                     GRIGDSPVIGAGLFLDNEVGASTCTGLGEAVMRTLASHTAVEEMRRGASPQEACEEAI
                     NRIVKKHPDYKEFQVGILAINKRGEHGAYGLQKGFNYALYQHDANKLYDADYFIKS"
     gene            11241..12203
                     /locus_tag="VFU15_05230"
     CDS             11241..12203
                     /locus_tag="VFU15_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007568014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEU4717209.1"
                     /translation="MDVCAGIDIGGTNTELGLVSADGSVLWLTTFPTRSMAKPEMLLG
                     FCTDALRKIISEKKLVLKGIGIGAPSGNYYTGSIEHAPNMPWPGIIPLADMFRGAMDV
                     PVVLTNDANAAAIGEMFFGAAKGLRDFAVITLGTGLGSGIVTGGEVLYGHDGFAGELG
                     HVIIEHEGRLCGCGRRGCLETYVSATGIVNTAKEWLEFEGEPTTLKTVSQEKIDSIMI
                     TDAAKAGDAFAMRLINYTAEKLGLALANLVAITSPSHIFLYGGLANAGDILMNATRSY
                     FEKSLLKNYAGKVKIEFSGLPGNAAVIGAAALAWNEKRAQSSNR"
     gene            12391..12579
                     /locus_tag="VFU15_05235"
     CDS             12391..12579
                     /locus_tag="VFU15_05235"
                     /inference="COORDINATES: protein motif:HMM:NF039593.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HEU4717210.1"
                     /translation="MKKLLLIALLSVNVFAVTSCGEKAKAAPKYQCPMQCEGDKTYDK
                     PGKCPECGMEMKEVKEDK"
     gene            12590..13255
                     /locus_tag="VFU15_05240"
     CDS             12590..13255
                     /locus_tag="VFU15_05240"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEU4717211.1"
                     /translation="MKRLLKRTASFFWRPVVARYLRKERRFRFENFELTVPPGVFHPG
                     FFFSTKELVRYLRPLDLRGKKFLETGCGSGLVSIAAAAQKAEVTAVDISISAVECTKK
                     NFERNRQKAIIFHSDLFASVPPGTFDIIAVNPPYYKKKALREEDHAWNCGEHMEYFLR
                     FFSEAKRFASAETQLVMVLSDESDNDAICSIAAKEGWRPSSVQRKGNLFGETLVFVFT
                     KQG"
     gene            13335..14327
                     /locus_tag="VFU15_05245"
     CDS             13335..14327
                     /locus_tag="VFU15_05245"
                     /inference="COORDINATES: protein motif:HMM:NF014021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEU4717212.1"
                     /translation="MQLHPEEIPRFYPDATDVAVLHTLIYFDIFHHPLTTDEVHHNCQ
                     WEKCTLSETALALEKMREAGIVKETDGFWLFTGREHLAALRRERNERALLCGKKARRY
                     SKLIASFPFVRGVFISGSLSKGTMDPDGDIDYFILTEPGRLWVARTALVAFKKIFLFN
                     SKKYFCVNYFLDTENLSVPDRNLFVATEISFLKPMHGADVFGEFMDANQWTSLFYPNR
                     KKTATAGIAHLQPGMFKRFFEFVFRGKAGDRFDQLTRRVTVWFWKKKFRHVPAEDFEV
                     RFRSTKKTSKHHPNGFQERVKAAMEERRKSLGSEWNFSLPAQTWEWKTSLAEEK"
     gene            14264..15787
                     /locus_tag="VFU15_05250"
     CDS             14264..15787
                     /locus_tag="VFU15_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEU4717213.1"
                     /translation="MELQPAGADVGMENVPRGGKVMNILFSHSYFMRFDPKQWEQGQP
                     FAPLGTLLAAAVLREKGHDVHLFDPQFSYDAEEVIPFLEKYSPDVFVIFDDGFNYLTK
                     MCLTNMREAAFRMQELAKEKGCRVIVCSSDSSDHAEKYLARGADFVIRGEGEETLAEL
                     VDAIGKKLPFETISGISLMKDGALFSTPKRNVMKDLDALPLPAWDLAGTGKYKAVWKE
                     KHGYFSINIATTRGCPYKCNWCAKPIYGNRYNSRSPENVIAELKMLKEKTAFDHVWFC
                     DDIFGLKPGWVKRFAELVKENDLHFTYKIQTRADLLVQENYVRDLAASGCETAWMGAE
                     SGSQKILDAMDKGTTVEQIREATYLLKKHGIRPAFFLQFGYPGETMEDIRKTIALLNE
                     LLPDDIGISVSYPLPGTKFYENVKAEMNAKQNWSDSDDLALMFRHTFSPEFYRQLHRY
                     VHRNYRKHQGYESLKQFVVAPWKVKKKDFRRAASVFWYLPVVQKERKRLAALSHEQH"
     gene            15774..>16569
                     /locus_tag="VFU15_05255"
     CDS             15774..>16569
                     /locus_tag="VFU15_05255"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU4717214.1"
                     /translation="MSSTETVFNEYAPFYDEHFTRSLTGTMQRKRVWKFLSRNISVKT
                     HRNVLELNCGTGEDALWFSDRGFNITATDLSPEMVAVTKEKLQGFAQVAFVSDIRDVA
                     SQCGGRKFNLVFSDFGGMNCLGPDELKKCAAQFSGLLETGGRLVFVVMSRNCRWEQRY
                     FRRKDDRESAYRRRSKEGIGANVFGQEIFTWYYSPEEFAGLFAGFFRVRKFRPIGYAI
                     PPSYLDRWLAKRKLLAKLLGLAESVCGNFSRLSDKADHFIIDLEKIK"
BASE COUNT         3922 a         4307 c         4693 g         3647 t
ORIGIN      
        1 gtttcctttt cgctgaacat gatgggcgga aacacgttcg cgcagcacgg gcacaccggc
       61 aaaggcgtcg tgatcgcgat ccttgatgcg ggtttcagcg gggcggattc ccacgaagcg
      121 ttccggcagc tgggacttaa cggacagatc cgcgcaacgt atgatttcgt cggtaagacc
      181 agcgatgtgt accactgcaa atcggaccac ggcacggaag ttttttcctg catcgccggg
      241 aagatgggcg ggaaaaatat gggtttcgcg cccgacgcga cattcctgct ggcccggatc
      301 atgaaggaat acggcaacga attccgcggg gaagaaaatt ttgtcgccgg ggtggaatgg
      361 tccgacaaga acggtgcgac gctgatcaac agcagcgccg gtccgaacga tttcgagtac
      421 atgcccgaag agatggacgg aaagaccgcg ctcatatcac gtgccgcgaa cctggcggcg
      481 agaaaaggca tgctcgtcat cgccgcagca ggaaacgaag gcggaacacg cgatgaaaat
      541 cttcttccgc cggcagactc cgacagcgtg ctggcggtaa cggcagtgaa cgacaccggt
      601 ttcattgctg attacagctc ggtcggtccc gtatttgatt tccgcacgcg aaagccggat
      661 gtctgcgcgc cgggaacagc gatcgttgcg tacggcgaca atgaatacgg tatcggcgac
      721 ggcacatcct tcagttgtcc tttcgtcgtt ggtttcgccg cgtgcctcgt tgaaatgtat
      781 cccgacatca cgccgctggg actcatggac acactccggc attccggctc gctctacccg
      841 tatttcgatt atgcgcacgg caacggaatt ccgcaggcgt cctatttctt cgatccgccc
      901 ggggccatgc cggaaacatt ttccttcggt acgcacgaca gctatacgga catcatcatc
      961 gatgataatt tcatgcccga ctgctgccgg aaaatgccca cgcttctttt ctacaatctc
     1021 gaaacggctt ccggaaagat ctaccggtac ggcgtcatcg aagtgcgaag caaaaaaccg
     1081 ctgtcgatcg acaacctgca ggacgtcaca cccggaacaa agctgaacgt ttcctacaaa
     1141 ggatattatg cgtcgaaagt tttctgacat agcgctcatc ttcattgccg tttttgtttt
     1201 ttctacggga aaaacggaag cgcagcgcgt gatatacgag aactccaccg atacgacggc
     1261 agccattccg aaagtgggcc cgaaccggct gttctttttc ttcccgacct attatttgtg
     1321 caccgaatct ttcccggtga gctacggcta cccggctgca ttctggagcg cgggcgccgg
     1381 catcgatctg aacttcaagc tgaaattgtg gtcgtgggaa tcgctggtgt ggagcgcggg
     1441 tttgcggcat gatcatttcg gcatcagcca gaagcaggta aagctgccgc cgctttcccc
     1501 cgatcgccac aagcgggaga aaatcgttac ggataatttc acgttttctt tctccgaccg
     1561 catcaatttc ggcaagcggg gaaatgtcat cggggcgtat gcggaattcg gcgtgaccgg
     1621 ttcatgcgta ttccgcacat cgtatgtcta tgtcgaccag tatttcgatt ccaacggccc
     1681 gggcgggcgg cagaacaaag tgaaaataaa atccacggga ctggcttacc tcgagccgtt
     1741 cacgtatggc ccggttttcc gcatcgggat ggaatattat tccatttccc tgcaataccg
     1801 ttacaacaac cttatccgtt cagcgtattc cccgaacggg caggacctgc cgaagctgat
     1861 gctgagcttc gaatacagca gggcgtactg aacctatcct gaccaatgaa aaaaacgggg
     1921 cgcggtgatt ctttcgcatt gctgaaaacc aaagaggaga tccgcgcaat aacctggcag
     1981 catttctctt tccaggttca tttttcatga gcaaccgttc cccggtttca ataattgtct
     2041 ctccgggcaa ctcccgtctt ttccggcgca tatcgtatat ttaaaggatc gtacagcaga
     2101 cagacagcca caccggaaaa gatattatgg agccaacacg tgttttcgac cttcttgatt
     2161 tcacaccttc gcatttcggg aacaaagccg atttcattac cgccaaagag aatgggaaat
     2221 gggtgaatta cgatacggaa accatcatcc gttactgcca cgaaatggcg tccgggttca
     2281 tcggtcatgg actgcagcgc ggggagcgcg tggccatcct cgcgaataac cggcccgaat
     2341 ggaacatcac cgattttgcc gtgcagcttt ccggcgcagt gctggttcct gtttacccga
     2401 cgatctccga gcacgacctg aattttatcc tgaaagattg cgaagcacgt tttgttttcg
     2461 tttcgaacaa tgacattttc ctgaaaacga aaacagcctg tgccggtctt gcggaccctc
     2521 cccgtatttt ctgctttgat gaagtggaag gaggaacatc gtggaaggag gtgatggata
     2581 ccggcaaaaa gaatccccgt gaagacgtca tcggcgaagt gaaaaaaaac acgaagcccg
     2641 gcgatatgtt cacgatactt tacacgtccg gaacaacagg cacgccgaaa ggagtcatgc
     2701 tctcgcaccg caacctcgtc agcaatttta cgtcgctctg cgatcttccg ccggtggatg
     2761 aaacgagcag ggtgctcagc ttccttccgc tcaaccatat ttacgagcgc atgctggtgt
     2821 acctgtattt ctacaaaggt ccttccattt attacgcgga aagtcttgaa aagatcgcgg
     2881 aaaatatccg cgaggtgaag ccgcacatgt tcacgtgcgt tccgcacctc gtgcagaaag
     2941 tatacaacgg catcacggcg aagggagcgg aacagaaagg gatcaaacgc tggctgtttt
     3001 tcacagcagt ggaaatggcc gagaagttcg agatcaggcc cggcggcaac agcttttttt
     3061 accgcctgaa gatggcgata ctcggcaggt tcgttttcag caaatggagg gcgggactcg
     3121 gcgggaacct gcgcgtgatg gtttccggcg gcgctgcgct cgaaccgaag ctggcgcgca
     3181 ttttctgggc ggcgggtttg ccggtgctgg aaggttacgg acttacagaa acatcaccgg
     3241 tgatcgcggt gaataatttc ggaccggaca acgtgcgctt cgggacggta ggacccgtga
     3301 tcaagggagt ggaagtgaaa tttgcagaag acggcgaaat cctcgtgaaa ggtccgaacg
     3361 tcatgctcgg ttattacaaa cgtcccgatg cgacggcgga agtgatcgac aaagacggct
     3421 atttccacac cggcgatatc ggcgaaatga tggaagggaa attcctccgc atcactgacc
     3481 gtaaaaaaga gatattcaaa acatccggcg gaaaatacat tgcgccgcag ctgatcgaaa
     3541 accttctccg cacatcgcgt ttcgtggagc aggcgatggt gatcggcgag aaccggaaat
     3601 tcccggcggc gctcatcgtg ccttcattca cattccttcg cgcttatgcc aagtacaaag
     3661 gcatacagac cggcatgagc aacgaagaac tgatcagcaa tgaaacgatc cgcaaacgca
     3721 tcgaggcatc ggtggaaaaa ctcaacgcgg agctcgccca gtatgaaaaa gtgaagaaga
     3781 tcattctcat accttcggag tggacggtga acagcgctga gctcacgccg aaactttcac
     3841 tccgtcgtaa agtgatcctg aaaaataacg aagctgcgat agagaagatg tacgaggaag
     3901 cggaccgcgt cagtgagaaa aaataaattt cccgaatagc aaaagggcga gcaatcccgg
     3961 gagcagcgct ttcgtcagga gctcgtacgt aaagatcttc ccggcgttcc gctgaccgta
     4021 tggcgtgtcg tcgatcttcg cgggcacgac attctgcagg gcattctgcg tcgttccgcc
     4081 cgttgcgttc gccgctgtgc ccccggccga ttccgcgaat ttctggacct gctcgggaac
     4141 tttttcgccg aagaaaacaa cggagaccgt gatgccttcg tccttcaccg cggcgcggat
     4201 ctttttcttc tcttttttgt cgaggtagat ctgcccgtcc gtcagcacga cgattttatt
     4261 cgttccgccg gggatcattt tcttccgcac cgatgcgtat gccgcatcga acgcgccttt
     4321 cgcatttgtc gcaccgcccg cggaaagatg atcgagcgca tcgcgtatcg tcgccgtgtc
     4381 ggaagcaggc acctgttcgc agagcacctg cacgttcccg ctgaacgcaa gtatggtcac
     4441 atgatcgttc accgaaagcg tgttcacgaa cggaagaatg gatcttttcg cttcatcgat
     4501 cagccctttc tgctgcatcg atcctgaaac gtcaatgaga aaaacgacat tgtcgcgcgg
     4561 cacggtatcg cgcggcgtca tgcacaccgg ctggccggga agcacttccg tcaggctcac
     4621 gtcgtcgacg tagtagtagg aatatttgtt gaacggcgcg acgcgcaggc cgccgtgctg
     4681 cgaacgtttc acgctgtgca cgcgtttcgg gaaatcaccc gcgaacgtgc cgagcgtgag
     4741 gaagcgttca ccgcctttcg caacgaacgt gtcgcacacc atcacccatc cttcgtcgct
     4801 ggtgatgtaa tggtcgttgc tgttgatcac ctgcgcgtac ggatcatagc cccacatgca
     4861 ggcgtggtag ttcgacacgg tgacgcagtc tttggtgatc gccgcaccga aagcgtggca
     4921 ggcgaaattg gatttcctgt cgagcgcgac ataatattcc acgcggtacg ttttccccgc
     4981 ttcgagcggc tgcagcaaac ggcattcgac atattctgcg tacgtatcgg aatagagcag
     5041 ccaggagaca aggctgtttt tggcaagtcc gccgatgatg gccattctcc cgtcgccggt
     5101 gcgcgcggtc tttgttttcc atcccagcag ccacgactga tccgagttga ggtaatccgg
     5161 cgtgccgtcc gtcacctgga accagtcttt gcagaggtaa tttttcgtgc ggtaatcgag
     5221 cgaatccctt ttccagtctt tgtaacgccc ccagttgaat ttctggatca ccggtgcgcc
     5281 gggttcttca aaggaaggat tgggaacgag gttttgctgc gctcctgtgc aaaccgaaaa
     5341 taaaagaagg taaaacagga aagtaaatct gcgcatcagc acgaaggtac aagaagtatg
     5401 ccctttgcgg gctccgggtt ttcccggttg ttttgaaatg gttttcggtc agtcgcctgc
     5461 tgtcagccgt tccgaacccc aaaaccggaa cttgctccaa catttcaact ttccaattgt
     5521 tcaattattt catccgcgca aacaacccat tgaatcccgg atcgctgtgc aggtaattga
     5581 acacggggtc gttctgcagg cggagccggt cgttgaagcc gagatcgatc gctttttcga
     5641 gattgggaac cacatacttg tcgagtccga gcgacatgta cagcgcagct ttcatgtacg
     5701 cccattcgct gttctccggg tcgacgatgc tgtagatcgt gatgagcgat tccgctgcat
     5761 gaagattgct cactttcatc gcctggttgc tgtaggtata gagcatgagg ctcgtgtacc
     5821 cgcgcaggcg ctggcgcatg aatttttccg caccggtcga tttcgtttcg aaatagctgt
     5881 ccgcatttgc gcgccaccac gtcgtgtctt tgtcgcgaac agcttcgccc agctcctgct
     5941 gcagcacagt ctcttcggct tccgcgtttt tccatcccgt ctcgtccgat cttacgcacg
     6001 ggtctttgct gatcgcggca aatttcttcg aaatattttc tttcccgggc gatgaggtga
     6061 caatgtgcgt tcccgtttcg agcaggttct tcgccagcag gcagctcatg cttttcatgg
     6121 aatcgctttg cgagatgagc gcttcggaca gggcattcat cgcattcgtg gaatcgaccg
     6181 ggtggatgct cttgtctttc ttgtagcagt tggtgatgag cagcgcggtg cgcattacgc
     6241 tcacgggcgc ccattcgtgt ttgccgtcga agacgagcag ctcgtgcggg tttgcattcg
     6301 cgggatagct gtcgttgaat tttttcatct cgaggtaatt catgtcgccg agcccggcaa
     6361 gaccgacaaa tattttgtcg cggaacgggg ccgccggcgc ggcagaattg cagatgatcg
     6421 ctttgaggtc gctgggactc ccgccgagca tggaaacgac gcgtgcgccg ccggaaaacc
     6481 cgcagagaat gatttcgtta tcggcgcagg ggtaaaggct tttcacttcg tcgatgagcc
     6541 cgttgccgat ggaagccgct tcgcccatct gtatgccgtt gcgcgaagag ttggatgcaa
     6601 cgaaaatgaa accgagctcg tcggcgatgg atttgtattt gtccagcggc tcatgcccgt
     6661 cgccgtgcgc gtcgaaaaag atcacgaccg gccattgttt gtcgtacgaa aaatccgaag
     6721 gaagataaac gctgaacgag ttcgccggct gggagcgcac agaaaggtcg gtgatcgtcc
     6781 ctggttttac ggccggtgct ttcggcggtt gctgctgggc gacggtatcg tcttttttgt
     6841 catccttgtc gtctgagatg caggaggcga ggaaagcgag gaagaggaga ggaagaagct
     6901 tgcgcatggg caaatatagg taaggttcaa ggttcggcgt tcagcggtta acgtccggga
     6961 aacaacgccc ggatggatgc ataccaggcg gcagacgttc cgccataaac ctgtaacgtt
     7021 tttggtatct ttcgggaacc attttccaga tgaaattccg ctgtgttttt gccttttttg
     7081 tgattgcgtc ggcgtggctc tcctgcattc cttacccgga cgagcattct ccgaaagtgt
     7141 atttccgctg ggacgttcct gaaccgaaat tcgattttgc gaaatacgtc gatccgctga
     7201 tcggcaccgg cggacacggg catacgtttc ccggcgcgtg cgcaccgttc ggaatggtgc
     7261 agctgagccc ggatacgcgc gtggacggaa gctgggacgg atgctcgggc tatcattacg
     7321 acgacgatac gatctatgga ttttcgcaca cgcacctcag cggaaccggc tgcagtgact
     7381 acggcgatat cctgttcatg ccggagaacg aagtcatctt caataccggc gccgacggaa
     7441 aaccgggcta ccgttcttca ttttcgcacc ggaacgaaaa agcggaagcg ggttactatt
     7501 ccgtcgttct cgataatacg aaaacaaagg cggagctgac cgcttcaccg cgcgtgggcg
     7561 ttcaccgcta cacattcagc ggcgacaacg agaactggat gctgctcgac ctcacgcacc
     7621 gcgacgaagt gcttgattcg aaagtggaga tcgtgaacaa gcacacgata cagggattcc
     7681 gccgttcgaa agcgtgggcc gaagaccagt gcgtttattt ttacgcggaa ttttccgaag
     7741 atttcgatga ataccgcgtc ggcgggcaga agggatacca gccggaagtc agcgtcgtga
     7801 aagggcacaa ggtgaagatc gcattcagct tcggcggaac aaaaaatctc gtgctgaaag
     7861 tggcgctttc accggtgtcg tgcgaaaatg caaagctgaa catgcaggcc gaagtgccgg
     7921 gcttcgattt tgatgccgtg cataaaaaag ttgtgcagca atggaacaat gagctcggga
     7981 agatcgaagt gaaaagcggt gacgaaacga aaaagaaaat tttctacacg gcgctgtatc
     8041 actgcttcat cgcaccgaac ctctattgcg acgtcaacgg cgattaccgc gggatggatg
     8101 accaggttca cagcgacacg gtccatccgc agtacacggt tttttcattg tgggacacgt
     8161 accgcgcgct tcacccgctg ttcacgatcg tgcagccgca gcgcgaccag gattttatcg
     8221 ccacgttcgt gaagcaggcg caggaaggag gacggctgcc ggtgtgggaa ttgtccgcga
     8281 atgaaacgaa ctgcatgatc ggctatcatt ccgttcctgt gatcgtcgac gcgtggatga
     8341 aaggactgac caatttcgac accgctacgg cgtacgaagc catgaaagat gccgcgatgc
     8401 gcgatgagcg cggactggcg tcgtacaaaa agttcgggtt cgttccggcc gaaggagaaa
     8461 gcgagtccgt ttccaaaacg ctggaatacg cgtacgacga ctggtgcatt gcacaggcgg
     8521 caaaacgcat gaagcgcgat gacgattatg cgtacttcat gcatcgttcg caatcgtgga
     8581 ggaacgtgta cgatccgcag acgggtttca tgcgcgcacg gctgaacaac tcattcgttt
     8641 ccccgttcga tccgggcgaa gtgaatttcc attacaccga agcgaacgcg tggcagtatt
     8701 cgctcgccgt tcagcaggat atcccgggac tcattgcagc gcacgggggt gacgagaaat
     8761 tcatcgcttt cctcgatcaa ttgttttcgg cttcgtcgca aacgtccggt cgcaaacagg
     8821 cggatatttc gggaatgatc gggcagtacg cgcagggcaa tgagccgagc catcacatgg
     8881 cgtatttata cgattacacc tcgcaaccct ggaaaacgca gcagctcgtt tcgcagatat
     8941 gcaatgaaat gtacacggac aaaccggacg gactctgcgg caatgaagac tgcgggcaga
     9001 tgagcgcgtg gtacgtgatg agcatgatgg gactttatga cgtgacgccg ggtacaaatg
     9061 agtacgctat cacccggcct ggttttgatc aggtcgaaat tcatcttgaa tcaggaaaaa
     9121 atttcgagat caatgatttg accaatgata cttttcagtt gaaatttccc gaaggtttta
     9181 aacccggata tgtttttgcg aatgtgaatg gcaacgcgat ccataaaaat gagatttcat
     9241 tcagtgatat ggcaaacggc gggaagtttg actttatcag gaccggaacc aactggcagc
     9301 aggtgaacat ttgcgaggac aataccacca tcccgctgcc gccacgcgtg aagtccgcca
     9361 gcgaaggccc ggtaaccccg atgcctttgc ggacattcgt gaacaggctc gatgtcacgt
     9421 acgagaacat tcccggcctg cgctattttc agagcgtgaa taacggtccg tatgtggaaa
     9481 ccggcactca ttttactatc aacggatccg gcattatccg tgcattcact gtcaatgaaa
     9541 cagggcggca cagcgatacg gttatgttca ggtatatgaa aattccgggc ggacgttcca
     9601 taacgctgaa tacggcttac gcgaatcagt atgcggccgg cggaaataaa gcgctcatcg
     9661 acggcatacg cggcgcggag aatttccgta ccggcgactg gcagggttac gaaggtgatg
     9721 acgtggacgc tgtggtcgat ctcggaagcg taaagaatgt cgcgcacatt tccatcggct
     9781 gcctgcagga ccaggcgagc tggatcttca tgccgcagga agttgatttt tatgtttcgg
     9841 acgacggcag gaatttcacg caggcgggaa gcatgatgaa caccatcgac gagcaccacg
     9901 acggccccgt cctgcacgaa ttctattgcg accccggcat gcccgcacgt tatgtgaagg
     9961 tcgtggcgaa gaaccgcggt gcatgtcctg catggcatcc gggcaaagga aaccccgcgt
    10021 ggatctttgc ggatgaaatt gagattgaaa cgcggtgatg gagcattccc tgtttgccgt
    10081 tttcatccgc gccggatcaa aacgttgaat aactgatcag gagtaataaa agagtcacat
    10141 gaacagaaaa atttttctcc agcgcaccgc cctctttact gctgccacct ccatgggtgt
    10201 cagtcctttt tccaaggacg atatccggag aaaggaaaag aagaacccga attcctcccc
    10261 gggattcccc gtggtcattt ccacctggaa ccacggcatg ccggccaatg aggaagcgtg
    10321 gaaaacgttg agcagcggcg gttccgcgct cgatgcagcg gagaaaggcg tcatggtcgt
    10381 tgagtccgac aagaacagca cctccgtcgg cctgggcgga tttcctgacc gcgacgggta
    10441 cgttacgctc gacgcgagca tcatgcgcga cgacggcatg gcgggctccg caagctttgt
    10501 caccggtatc gagcacccga tctcgctcgc acgcatgatc atggaacgca cgccgcacgt
    10561 gatgctcgtg ggaagcggtg cggaacagtt cgcacgcgag cagggcatca cgcagctgcc
    10621 gaacaagctg agtcccgccg ctgaaaaagc atggaaagac tggctgaagg aaaaaaacta
    10681 caagccggtg atcaacgtgg agaaccacga cacgatcggg ctgcttgttc tcgacaaaga
    10741 cgggaagttc gccggctcat gcacgacgag cgggcttgca tggaagctgc atggccgtat
    10801 cggtgattcg ccggtgatcg gcgccgggct tttcctggac aatgaagtgg gtgcttctac
    10861 ctgcacgggt ctcggggaag ccgtcatgcg cacgctggcc tcgcacacag cggtggaaga
    10921 aatgcggcgc ggcgcgagcc cgcaggaagc atgcgaggaa gccatcaacc gcattgtgaa
    10981 aaagcatccc gattacaaag aattccaggt gggcatactc gccatcaaca agcggggcga
    11041 gcacggcgct tacgggttgc agaagggatt taactacgct ctttaccagc atgacgcgaa
    11101 caagttgtac gatgcggatt atttcataaa gtcctgaagc acgtccggcg ggaaagaaaa
    11161 caggttgggc gaaaacgtgt aacggcagca gaaaatgttt ttattttagg aaacaacgaa
    11221 aaggcagaaa ggaagcgctt atggatgttt gtgcaggaat agatattggg ggtacgaata
    11281 cggaactggg acttgtttcc gcggacggaa gcgtgttatg gctcaccaca ttcccgacgc
    11341 gctcaatggc gaaaccggaa atgctgctcg ggttctgcac ggatgcgctg cggaagatca
    11401 tctccgaaaa aaaactggtg ctgaaaggga tcggtatcgg tgcgccgagc gggaattatt
    11461 acaccggcag catcgagcac gcgcccaaca tgccctggcc gggtatcatt ccgctggcgg
    11521 atatgttccg cggcgcaatg gatgtgccgg tcgtgctgac gaacgacgcg aacgctgccg
    11581 ccatcggtga aatgtttttc ggtgcagcga aaggactgcg cgattttgcc gtcatcacgc
    11641 tggggacggg actcggaagc gggatcgtta ccggcggcga agtgctgtac gggcacgacg
    11701 gttttgccgg agagctcggc cacgtgatca tcgagcacga aggaagactg tgcgggtgcg
    11761 gaagaagagg ctgtctcgaa acctatgttt ccgcaacggg catcgtgaac acggcgaaag
    11821 aatggctcga gttcgaaggc gaacccacga cgctgaaaac cgtttcgcag gaaaagatcg
    11881 acagcatcat gatcacggat gccgccaaag ccggtgatgc attcgcgatg cggctgatca
    11941 attacacggc ggagaagctg ggacttgcgc tggcgaacct tgttgctatt accagtcctt
    12001 cgcatatttt tctttacggc ggacttgcca atgcgggaga tatcctcatg aacgcgacgc
    12061 gttcgtattt cgaaaaaagc ctgcttaaaa attatgcagg aaaagtgaag atcgaattct
    12121 cgggactgcc gggaaatgcg gccgtgatcg gcgccgcggc gctggcctgg aatgagaagc
    12181 gggcgcaatc gtccaacaga tagtggcgat ctgcagtaaa cggcagcggc tcgctttcgg
    12241 aatagatccc ggttcccgtg tctttgtgtc aaaagaagca ggctccggag gttaacggtg
    12301 aaaattcaaa cgtgccggcc ggttccgaag cgaaaaacgt cggaatcatt tctgcaatgc
    12361 gtaaatttgt acttccctaa aacaacgaac atgaaaaagc tcctgctgat cgccctgctt
    12421 tccgtgaatg ttttcgccgt tacttcctgc ggagaaaaag cgaaagccgc gccgaaatac
    12481 cagtgtccca tgcaatgtga aggcgacaag acctacgaca aaccgggcaa atgtcccgag
    12541 tgcggcatgg aaatgaagga agtgaaagag gataaataat ccgcatcctt tgaaacgcct
    12601 gctgaagaga acggccagct ttttctggcg acccgttgtc gccaggtacc tgcggaaaga
    12661 acgtcgcttc cgctttgaaa atttcgagct tacggtcccg ccgggtgttt ttcatcccgg
    12721 cttttttttc agcaccaaag agctggtccg ttacctgcgc ccgcttgacc tgcgcgggaa
    12781 gaaatttctc gaaacaggtt gcggctctgg tcttgtgagc attgccgccg cagcacagaa
    12841 agcagaagtg acggccgtgg atatttccat ttccgctgtg gaatgcacga aaaaaaattt
    12901 tgagcgcaac aggcaaaaag caatcatttt ccattcggat ctttttgcgt ccgttccgcc
    12961 gggaacgttc gatatcatcg cggtgaaccc gccgtactac aaaaagaaag cgctgcggga
    13021 ggaagatcat gcatggaact gcggcgagca catggaatat ttcctccgtt ttttttcaga
    13081 agcgaagcgg ttcgcttccg cggaaacgca gctcgtcatg gtgctttccg atgaatccga
    13141 caatgatgcg atctgttcga tcgcagcgaa agaaggatgg aggccgtcat ctgtgcagcg
    13201 gaaaggaaat ctcttcgggg aaacgctggt gttcgtcttt acaaaacagg gttgatcttt
    13261 ccatccggcc ggagaagcga ccgctcttcc ctttatccgc gactttactt ttaaagttgt
    13321 aaatttcaac atccatgcag cttcacccgg aggaaattcc gcgcttttat cccgatgcga
    13381 ccgatgtcgc tgtgctgcat acgctcatct acttcgatat ttttcatcac ccgctcacga
    13441 ccgatgaagt gcatcacaat tgccagtggg aaaaatgcac gctttcggaa accgcgctcg
    13501 cgctggaaaa aatgcgcgag gcaggcatcg tgaaggagac ggacggcttc tggctgttca
    13561 cgggccgcga gcatcttgcc gccctgcgca gggagcgcaa cgaacgcgcg ctgctgtgcg
    13621 ggaagaaagc gcggcggtat tcgaagctga tcgcttcatt cccgttcgtg cgcggcgtct
    13681 tcatcagcgg ttcgctttcc aagggaacaa tggaccccga cggcgacatc gattatttca
    13741 tactgacgga accgggacga ttatgggtcg cccgtactgc gctcgtcgcc ttcaagaaaa
    13801 tattcctgtt caattcgaag aaatatttct gcgtgaatta ttttctcgat acggagaacc
    13861 tgtccgttcc cgaccgcaat ctttttgtcg ccacggagat cagcttcctc aaaccgatgc
    13921 acggggcgga tgtgttcggt gaattcatgg acgccaacca gtggacgagc ctcttttacc
    13981 cgaaccgtaa aaagacggca acggccggca ttgcgcacct gcagccggga atgttcaaac
    14041 ggtttttcga atttgttttt cgcgggaaag cgggcgaccg cttcgatcag ctcacgcgca
    14101 gggtgacggt gtggttctgg aagaaaaaat tccggcatgt gcctgcggaa gatttcgagg
    14161 tgcgcttccg ttcgacgaaa aaaacatcga agcatcatcc gaacggtttc caggaacgcg
    14221 taaaagcggc gatggaagaa cgcagaaaat cgctcggcag cgaatggaac ttcagcctgc
    14281 cggcgcagac gtgggaatgg aaaacgtccc tcgcggagga aaagtgatga acattctttt
    14341 ttcacattcg tattttatgc gtttcgatcc caagcaatgg gagcaggggc agccgttcgc
    14401 gccgctggga acgctgcttg ctgcggctgt gctgcgtgaa aaagggcacg acgttcatct
    14461 cttcgatccc cagttttcgt acgatgcgga agaggtcatc cctttcctgg aaaaatattc
    14521 gccggatgtt ttcgtcatct tcgacgacgg gttcaactac ctgacgaaaa tgtgcctgac
    14581 gaatatgcgc gaggccgcgt tccggatgca ggagctcgcg aaggaaaaag gttgccgtgt
    14641 catcgtctgc agcagcgatt ccagcgacca tgcggaaaaa tacctcgccc gcggcgctga
    14701 ttttgtcatc cgcggcgaag gagaagaaac gctggcagaa ctggtggatg cgatcgggaa
    14761 aaagctgccg ttcgaaacta taagcggaat ttcgctgatg aaggacggcg ccctgttctc
    14821 cacgccgaaa cggaacgtga tgaaagatct cgacgcgctt ccgctgccgg cctgggatct
    14881 cgccggcacg ggcaaataca aagcggtgtg gaaagaaaag cacggatatt tttcgatcaa
    14941 catcgctacg acgcgcggtt gtccctacaa atgcaactgg tgcgcgaaac ctatttacgg
    15001 gaaccgttac aactcgcggt cgccggagaa cgtcattgct gagctgaaaa tgctgaagga
    15061 aaaaactgcg ttcgatcacg tatggttctg cgatgacatt ttcgggctga agcccggctg
    15121 ggtgaaacgt ttcgcggagc tggtgaagga aaatgatctt cacttcactt acaagataca
    15181 gacgcgcgcc gatctgctcg tgcaggaaaa ttacgtgcgc gatctcgccg cttccggctg
    15241 tgaaaccgcg tggatgggcg cggaaagcgg atcgcagaaa atactcgacg cgatggacaa
    15301 aggcacgacc gtggagcaga tccgcgaagc cacgtacctg ctgaaaaagc acggcatacg
    15361 gccggctttc ttcctgcagt tcggttaccc cggcgaaaca atggaagaca tccggaaaac
    15421 gatcgcgctg ctgaatgagc tgcttcccga cgacatcggc atctcggtca gctacccgct
    15481 cccgggaacg aaattctacg aaaacgtgaa agcggaaatg aatgccaagc agaactggtc
    15541 cgactcggac gatcttgcgc tgatgttccg gcatacgttc tcgccggaat tttaccggca
    15601 gctgcatcgc tatgttcacc ggaattaccg caagcaccag ggctacgaaa gcctgaagca
    15661 gttcgtcgtg gcgccgtgga aagtgaagaa aaaagatttc cggagagccg cttccgtgtt
    15721 ctggtacctg ccggtcgtgc aaaaggaaag gaaaagactg gccgcacttt cgcatgagca
    15781 gcactgaaac cgttttcaat gaatacgctc cgttctacga tgagcatttc acacgttcac
    15841 tgacgggaac gatgcagcgg aaacgcgtgt ggaaattcct ttcgcggaat atttctgtga
    15901 agacgcaccg caacgtgctc gagctgaatt gcggcacggg tgaagatgcg ctctggtttt
    15961 cggaccgcgg gttcaacatc accgcaaccg atctttcacc ggaaatggtg gcggtgacga
    16021 aggaaaagct gcagggattc gcgcaggtgg cttttgtttc ggacatccgc gatgtcgctt
    16081 cccagtgcgg cgggcgtaaa ttcaatcttg tgttctccga tttcggtgga atgaattgtc
    16141 tcggtccgga tgagctgaag aaatgtgctg cgcagttttc cgggttgctg gaaacgggcg
    16201 ggcggcttgt ttttgtcgtc atgtcgcgca actgcaggtg ggagcagcgg tatttcaggc
    16261 gaaaagatga tagggagtcg gcgtaccgtc gccgttcgaa ggaaggaatt ggcgcaaatg
    16321 tgttcgggca ggaaattttc acgtggtatt attccccgga agaattcgcc gggctgttcg
    16381 ccgggttttt ccgcgtaagg aaattccgtc ccatcggtta cgcgataccg ccgtcgtatc
    16441 tcgaccgctg gctggcgaag cggaagctgc tggcaaagct gctcgggctt gctgaatcgg
    16501 tctgcgggaa tttttcacgg ctctccgaca aagcggatca ttttatcatc gatctcgaaa
    16561 aaataaaat
//
LOCUS       DASTSF010000180         8140 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1426668,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000180 DASTSF010000000
VERSION     DASTSF010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 8140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8140
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1426668"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..801)
                     /locus_tag="VFU15_05260"
     CDS             complement(<1..801)
                     /locus_tag="VFU15_05260"
                     /inference="COORDINATES: protein motif:HMM:NF024842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU4717215.1"
                     /translation="MPKKVIILGGGVAGLSAAHELAERGFSVEIFERNPDVPGGKARS
                     VPVPGTMTDGKLPLPGEHGFRFFPGFYKHLPDTMKRIPFPGKKSVYENLVAPDRIMVA
                     RYDKPPILTVANFPHSISDVKLVIHDMFGGVDTGLTEEEIDFFAARVWQLMTSCYDRR
                     LNDYERIGWWEFCQADRFSETYRSLLVEGLTRTLVAAQAEIASTKTGGDIFLQLIFNM
                     MMPFVDTDRVLCGPTNEMWIYPWLVYLREKGVKYHLGAEAKSFECSDTA"
     gene            1146..1838
                     /locus_tag="VFU15_05265"
     CDS             1146..1838
                     /locus_tag="VFU15_05265"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEU4717216.1"
                     /translation="MKCIIVDDTAMAREMLKDLLSSYDEIELVAECSDTFQAKKILGE
                     KKIDLMLLDVEMPKVSGLEFLRSLENPPLTILITAREHYAVEAFEHNVVDYLVKPVNE
                     SRFMKAIGRAQELFRDRDKTVAGADKEYIFIKDKGVLTKVLFSDILFIKALGDYVTIH
                     TPGKQYTIHVNMRSIEERLPADKFFRLHRSYIVALDKVDTIEQDTVFVQKNAIPIGEA
                     QKPAMLARLNLL"
     gene            1972..2349
                     /locus_tag="VFU15_05270"
     CDS             1972..2349
                     /locus_tag="VFU15_05270"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU4717217.1"
                     /translation="MKILLIDDDALVLRSLSHQLAEEGYHVDTAKDGAEAVDYIEKEK
                     TRPDLIICDLLMPGISGLTFISLLRNFHNFSIPVIVISSLDKGYFLSQQAGIPGTDFL
                     PKPVEFSLLSGKIKKYIPKTVQL"
     gene            2346..2756
                     /locus_tag="VFU15_05275"
     CDS             2346..2756
                     /locus_tag="VFU15_05275"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU4717218.1"
                     /translation="MTRKTQMSIFIVEDNPMYSLYVDTLLKEQFNFKISTYESGEKCL
                     KDLYLTPDIIILDYVLGGIDGLAVLSVIKKQLPDTPVILLTGQKDINTAIQLMKAGAF
                     EYIIKDKDAPQKLADAIVRIEEEKSRKWKRFILV"
     gene            2759..3541
                     /locus_tag="VFU15_05280"
     CDS             2759..3541
                     /locus_tag="VFU15_05280"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU4717219.1"
                     /translation="MKKQKVLLVEDNELSSELGFSFLEEAGYDCRVAANGEEALHALR
                     SGDFDLVLMDVEMPVMDGLTATRKIRAELKKDVPVIALSGHTSAEEHEKCIAAGMNDC
                     VVKPFQKEALYAAIAKQLKKNGAAKKREEATAGSTAAGAVTDLTYLRSIARNNEGFFT
                     SMIRIFTEQTPEDVKKLDAAVHECDFETIRTLAHKIKTSVVFVGADAHVQRELVEMEK
                     LGEEKKSILRIAELFAHVKKTCTTACEELTLFLAGKNQKSSG"
     gene            3544..6951
                     /locus_tag="VFU15_05285"
     CDS             3544..6951
                     /locus_tag="VFU15_05285"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU4717220.1"
                     /translation="MPAKRKRLSVIPAFIVLLFCGTMNAQAGTEEFRARLFPITSNDG
                     LSQGFVSSMVQDHRGFIWFATKDGLNRYDGYSYKIYRHDPDDPFSVADNIVLNIFEDS
                     RGYLWISTPHILQVFDPVSEKFTPVPLLIGGKDYGAPAKIAEDHSGNMWGRASGELVY
                     LRLEKHPGKDDSSFAEKYTVTPFTAESVFGKQFVKESGNTNFFTDAEGDFWVTDSETV
                     YYSAAADLAAGRITAKYSQPEFCGMENARIIGASADTVRHTMTFISTGGFSVYDTKTD
                     KLLRTVPFPLTDITSFNAVAGDELGNAWVSTTTGVFIYSDSTRLVRKFMPLNIDSTVW
                     NTYIIRSLLVDRGGIVWMGANGFGVFKYNPRVSFFHLVDQPGISLIRGTHDGRIFISI
                     RGDLFSFDPVACKKGAPAFREKNFAQTFNGEQNFPQDFQQDAGGTFWLSYGHGTIGKY
                     DEKSGKISFFRLAVPGRDFEARGRIWLDPENNPWISAECGEDTYIARFNAATGTFDIT
                     AQVPGKKVNWMYTFVSDAFTDAAGNTWIGTTEGLYCYSVKTGQWKSYKNIPGNKSSLS
                     SDIIFSVCPDPHEPTKFIWVGTGSGGLNCLDVAHGTFAHFTDKDGLPNNVVYAVVPDG
                     AGNLWLSTNHGLCRFSVQAHTCKNYFASDGLQGNEFNRYVYYAADDGALYFGGVNGMN
                     FFYPAALGRSAHPPQLVFTELRLFNREMIPGDSGSVISKPIDFVRELVLPYDQNVITI
                     GFAAMDFSAPEKNQFRYKLEGFDKDWIFSGTRNEAIYTGLAPGDYRLLVTTANSEGTW
                     SAESRVIHIVIRHPWWKTWWATALFIFLGAWSVGMIILLRTKSLQRSRKILEEKVSER
                     TRELDETITNLRNTQKQLIKNEKLASFGQLTAGIAHEIKNPLNFINNFSELSNELIEE
                     LKLASTDEERNEIMLNLQNNIEKISFHGHRVDSIIRSMLNHSRTAGARRQPTDINKLC
                     GEFSNLAYHSMRANVQDFSCSLKSDYGKNIPELMVIPQDISRVLLNLLNNAFYAVNEK
                     RKTAGADYKPEVKISTRMQEEKVVISIRDNGSGIPAELMDKLFDPFFTTKPAGEGTGL
                     GLSITYDIIHSHGGKISVDSKAGEFTEFTIHLPVRTNGTAGAPSLKE"
     gene            6957..7340
                     /locus_tag="VFU15_05290"
     CDS             6957..7340
                     /locus_tag="VFU15_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007355046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU4717221.1"
                     /translation="MIKVLVVDDEKDVEFLFNLQFRKEIRAGKIKLYFAFSGEEALSF
                     MKTLDPFDLVLLMSDINMPGMTGLELLKATKENFPGLKVVMITAYSDARNRLAAEEGG
                     AVAFLTKPLNFEELRNILFNPNENS"
     gene            7340..>8140
                     /locus_tag="VFU15_05295"
     CDS             7340..>8140
                     /locus_tag="VFU15_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003444317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HEU4717222.1"
                     /translation="MAAKILVVDDESDLELLIRQKFRHAIKSGELLFDFALNGVEALK
                     KLEGDREYSMVITDINMPEMDGLTLLGKIGEMDRPLKAVVISAYGDMGNIRTAMNRGA
                     FDFITKPIDLKDLEVTIAKTLRETDAIRQGLAAKENLGKALFEKDKAEEAKRFEQQFL
                     ANMSHEIRTPMNAVIGMTNLVLKTELNERQRKYVDAIRVSSENLLGIINDILDISKIE
                     AGKIDLESIPFSVKDVLGNVYTVLHLKAEEKSLSLVMETGETVPQALLG"
BASE COUNT         2013 a         2193 c         2226 g         1708 t
ORIGIN      
        1 cgccgtatcg ctgcactcga agcttttcgc ttccgcgccg aggtgatact tcacgccttt
       61 ttcccggagg taaaccagcc acgggtagat ccacatttca ttggtgggcc cgcagagcac
      121 acggtctgtg tcgacgaacg gcatcatcat gttgaagatg agctgcagga aaatgtcgcc
      181 gcctgttttc gtgctggcga tctccgcctg cgcagcgacg agtgttctcg tcagcccttc
      241 caccagcagc gagcggtacg tctcggaaaa acggtcggcc tggcagaatt cccaccagcc
      301 gatgcgctca taatcattga gacgccggtc gtaacagctc gtcatgagct gccacacgcg
      361 cgctgcgaaa aaatcgatct cttcttccgt gagcccggtg tccacgccgc cgaacatgtc
      421 atggatcacc agcttcacat cgctgatcga atgcggaaaa ttcgcgacgg taaggatagg
      481 cggcttatcg tagcgcgcca ccatgatgcg gtcgggcgca acaaggtttt cataaacgga
      541 ttttttcccg gggaacggaa tgcgtttcat cgtgtcgggc aggtgcttgt agaatcccgg
      601 gaaaaagcgg aagccgtgct cacccggaag cggcagcttt ccgtcggtca tggttccggg
      661 aacgggcacg ctgcgtgctt ttccgccggg aacatccggg ttgcgttcaa aaatttcaac
      721 ggagaatccg cgttcggcaa gctcgtgcgc tgcgctgagc ccggcaacgc ctccgccaag
      781 aatgatcact tttttcggca tgggaatgag aataacaggt ggtacggatc atgcgtttga
      841 tctgaagatc gggtcacgca tgcaaccaaa attaaagatg ttttattcat acgcaaatcg
      901 aaaagcgcgt gttgaaagtc accggagcgg gtttcggcgc cgggattttc cggttgagca
      961 tttctttttt ccggttcgtc aaattccggg agccgtccgt aaacaagatg tggttgtatg
     1021 aatgatcttc gtgcttcttt agacgccgga cgggggtgaa accgtcgcgc atggaaaaaa
     1081 aatccgcggg ccgggaacgt gtccgtaaaa aaaacataat tgaactttta aattattttt
     1141 acatcatgaa gtgcataatc gtcgacgaca ccgcgatggc gcgtgagatg ctgaaggacc
     1201 ttctttcctc ttacgatgag atcgagctgg tggccgaatg ttccgacact ttccaggcga
     1261 aaaagatact cggcgaaaag aagatcgacc tcatgctgct ggatgtggag atgccgaaag
     1321 tgtcgggact cgaattcctg cgttcgcttg aaaatccccc gctgacgatc ctcatcaccg
     1381 cccgcgagca ttacgctgtc gaggcgttcg agcacaacgt ggtcgattac ctcgtcaaac
     1441 cggtgaacga aagccgcttc atgaaagcga tcggccgggc gcaggagctt ttccgcgacc
     1501 gcgacaagac cgtggccggc gccgataagg aatacatttt catcaaggac aagggcgtgc
     1561 tcaccaaagt cctgttcagc gatatccttt tcatcaaggc gctgggcgat tacgtcacca
     1621 tccacacacc gggaaagcaa tacacgatcc acgtcaacat gcgttccatc gaagaacgcc
     1681 tgccggccga taaattcttc cgtcttcacc gttcctacat tgtcgcactg gacaaggtgg
     1741 acacgatcga gcaggatacg gtttttgtgc agaagaacgc cattcccatc ggcgaagccc
     1801 agaaacccgc aatgctggcc cggctgaacc tgctctgatt cccgggattc ccgacctccg
     1861 ccccggcggt ttcgaccaca attttcgacc gtttcgtcaa ttttcccacc gggaagcggt
     1921 ccgggtagct acatttaaaa cacacaattc acttatacaa aaggaggtcg tatgaaaatt
     1981 ctgctcattg atgacgacgc attggtgctc cgctcgcttt ctcaccagct ggcggaagaa
     2041 ggataccacg tcgatacggc gaaagacggg gccgaagccg tcgattacat cgaaaaagaa
     2101 aaaacgcgtc ccgacctgat catctgcgac ctgctgatgc cgggcatttc aggactgacg
     2161 ttcatttctc ttctccggaa ttttcacaac ttcagcatcc cggtgatcgt gatctcctcg
     2221 ctcgacaaag gatatttcct ttcccagcag gccggcattc ccgggaccga tttccttccg
     2281 aagccggtgg aattttcact gctttccgga aaaataaaaa aatacattcc taaaaccgtg
     2341 cagttatgac caggaaaacc cagatgtcca tcttcatcgt tgaagataac ccgatgtatt
     2401 ccctttatgt ggatacactg ctgaaagaac agttcaattt caagatcagc acgtacgaga
     2461 gcggggagaa atgcctgaaa gacctttacc tcacgccgga catcatcatt ctcgattatg
     2521 tgctgggcgg gatcgacggg cttgcggtgc tttccgtgat caaaaagcaa ttgcccgata
     2581 cgccggtcat cctgcttacc ggccagaaag acatcaatac ggcgatacag ctcatgaaag
     2641 cgggtgcgtt cgaatacatc atcaaggaca aagacgcgcc gcagaagctc gccgatgcga
     2701 tcgtccgcat cgaggaggaa aagagccgca aatggaaacg cttcatcctt gtctgaaaat
     2761 gaaaaagcag aaggtgcttc ttgtggaaga taacgagctc agcagtgagc tcggtttttc
     2821 tttccttgaa gaagccggtt atgattgccg cgtggccgcc aacggggagg aagcgctgca
     2881 cgcgctgcgc agcggggatt tcgatcttgt cctgatggat gtcgaaatgc cggtgatgga
     2941 cggcctcacg gcaacgcgga agatccgcgc ggagctgaag aaggatgttc cggtgatcgc
     3001 gctttccggc cacacctcgg cggaagagca tgaaaaatgc atcgcggcgg gaatgaatga
     3061 ttgtgtcgtc aaaccgtttc agaaagaagc gctgtacgcc gcgatcgcga aacagctcaa
     3121 aaagaacggc gctgcaaaaa agagagagga agcgacggcc gggagcactg cggccggtgc
     3181 tgttaccgat ctcacttacc tgcgcagcat cgccaggaac aatgaaggtt tctttacatc
     3241 gatgatccgc atttttacgg agcagacacc ggaagacgtg aagaagctcg atgcggccgt
     3301 gcacgaatgt gatttcgaaa cgatccgcac gctggcgcac aagatcaaaa cctccgtcgt
     3361 gttcgtgggt gcggatgcgc acgtgcagcg cgagctcgtg gaaatggaaa agctcgggga
     3421 ggagaaaaaa agcatcctgc gcattgcaga attatttgcg cacgtaaaaa aaacgtgcac
     3481 gacggcctgt gaagagctga cgcttttcct tgccgggaaa aatcaaaaaa gcagcggttg
     3541 accatgccag cgaagaggaa acggttgtct gtcatacccg cattcattgt cctgctcttc
     3601 tgcggaacta tgaacgcgca ggccggtacg gaggaattcc gtgcgcggct tttccccatt
     3661 accagcaacg acggactttc acagggattc gtatcgagca tggtgcagga tcaccgcggg
     3721 ttcatctggt tcgccacgaa agacggcctc aacaggtacg acgggtattc ttacaagatc
     3781 taccgccacg atccggacga tccgttttcc gttgccgata atatcgtgct gaacattttc
     3841 gaagactcgc gcggctatct ctggatctct accccgcaca ttctccaggt cttcgacccg
     3901 gtttcggaaa agttcacgcc tgttcctctt ctcatcggtg gaaaagatta tggcgctccg
     3961 gcgaagatcg ccgaagacca ttcgggaaac atgtggggac gcgcttccgg cgagcttgtt
     4021 tacctgcgcc tggaaaaaca tcccggtaaa gacgatagct ctttcgctga aaaatacacc
     4081 gtgacccctt tcaccgctga aagcgttttc ggaaaacagt ttgtaaaaga gtccgggaac
     4141 acaaattttt ttacggatgc tgaaggcgat ttctgggtga cggattctga aacggtttat
     4201 tattcggcgg cagcagacct tgcggcgggc cggatcacag cgaaatacag ccagccggag
     4261 ttctgcggaa tggagaacgc ccggatcatc ggcgccagcg cggacacggt acggcacacg
     4321 atgacgttca tctccacggg aggattttcc gtttatgata caaagacgga caagctgctg
     4381 cgcacggtgc cgtttcccct gacggacatc acttccttca atgcggttgc aggcgatgag
     4441 ctgggcaatg cctgggtctc cacgacgacc ggcgttttca tttacagcga ttccacccgg
     4501 ctggtgcgga agttcatgcc gctgaacatc gacagcacgg tgtggaatac atacatcatc
     4561 cggtcgcttc tcgtcgaccg cggcggtatc gtctggatgg gcgccaacgg gttcggtgtg
     4621 tttaaataca acccgcgcgt ttcctttttc catcttgtcg accagcccgg aatttccctg
     4681 atcaggggaa cgcacgacgg ccgcattttc atttcgatcc gcggtgatct tttttccttc
     4741 gacccggtcg cctgcaaaaa aggcgcgccg gcattccggg aaaagaactt tgcgcagaca
     4801 ttcaacgggg agcagaattt tccgcaggat tttcagcagg acgctggcgg gacgttctgg
     4861 ctcagctacg gccacggaac catcgggaaa tacgacgaaa aaagcgggaa gatatcgttc
     4921 ttccgcctcg cggttcccgg ccgggatttc gaagcgcgcg gccgcatctg gctcgacccg
     4981 gaaaataatc cgtggatatc ggcggaatgc ggcgaagaca catacatcgc gcgtttcaat
     5041 gcggcaacgg gaacgttcga catcacggcg caggtgcccg ggaaaaaggt gaactggatg
     5101 tatacgttcg tgtcggatgc gttcacggat gccgccggca acacctggat cggcaccacg
     5161 gagggattgt attgttattc ggtgaaaacg gggcagtgga aatcgtataa gaacattccg
     5221 gggaataaat catcgctgag cagcgacatc attttttccg tttgccccga tccgcacgaa
     5281 ccgacgaaat tcatctgggt aggtaccggc agcggcggac tcaattgtct cgacgtcgcg
     5341 cacgggacat tcgcgcattt caccgacaag gacggccttc ccaacaacgt ggtgtacgcc
     5401 gtggtccctg acggtgcggg aaatctctgg ctcagcacga atcacgggtt gtgccggttc
     5461 agtgtgcagg cgcacacgtg caagaattat tttgcgagcg acggtctgca gggaaacgag
     5521 ttcaaccgct atgtatatta tgcggcagat gacggtgcac tgtatttcgg cggcgtgaac
     5581 gggatgaact ttttttatcc cgcagcgctc ggccgcagcg cgcatcctcc gcagctcgta
     5641 ttcacggagc tccgcctgtt caaccgggaa atgatacccg gcgattcggg ttcggtgatt
     5701 tcaaaaccca tcgatttcgt ccgtgaactt gttctccctt atgaccagaa cgtgatcacc
     5761 atcggttttg ccgcgatgga tttttccgct cccgaaaaga accagttccg ttacaagctg
     5821 gaagggttcg acaaagactg gatattttcc ggaacgcgca atgaggcgat ctacaccggg
     5881 cttgcaccgg gcgattaccg cctgctcgtc acgaccgcga acagcgaagg cacgtggagc
     5941 gccgaaagcc gcgtcattca tatcgtcatc cgccatccct ggtggaaaac atggtgggcg
     6001 accgcgctct ttatttttct cggcgcgtgg tcggtcggga tgatcatcct cctgcgcacg
     6061 aagagcctcc agcgcagccg caagatcctc gaagagaaag tgagcgaacg cacgcgcgag
     6121 ctcgatgaaa cgatcaccaa tctccggaac acgcagaagc agctcatcaa gaatgaaaag
     6181 ctcgcgtcgt tcggccagct gacggcgggc atcgcgcacg agataaaaaa cccgctcaac
     6241 ttcatcaaca atttctccga gctgagcaac gagctgatcg aagagctgaa gctggcttcc
     6301 acggatgagg aacgcaacga gatcatgctg aacctgcaga acaatatcga gaagatcagc
     6361 ttccacggtc accgcgtgga ttcgatcatc cggagcatgc tcaatcacag ccgcacggcc
     6421 ggcgcgcgcc gccagcctac agatatcaac aagctgtgcg gggaattctc gaatctcgcg
     6481 taccacagca tgcgcgccaa cgtgcaggat ttcagctgca gcctgaagtc ggattacgga
     6541 aaaaatattc cggagctcat ggtcatcccg caggacatca gccgcgtgct gctcaacctg
     6601 ctcaacaatg cgttctatgc cgtcaatgaa aaacggaaaa cggccggcgc cgattacaag
     6661 ccggaagtga agatctccac gcgcatgcag gaagagaaag tcgttatttc catccgcgac
     6721 aacggcagcg gaattcctgc ggaacttatg gacaagctgt tcgatccttt cttcacgacg
     6781 aaacctgccg gcgaaggaac aggcctcggc ctcagcatta cgtacgacat tatccactcg
     6841 cacggcggga aaatctccgt cgattcgaaa gcgggtgaat tcacggagtt taccattcat
     6901 ctcccggtcc gcacgaacgg aacggcaggt gcgccctcac ttaaagagta accattatga
     6961 tcaaagtact ggtagtcgat gatgaaaaag atgtggagtt cctgttcaat ctccagttca
     7021 ggaaagagat ccgcgccggg aaaataaaat tgtattttgc tttttccggc gaagaggcgc
     7081 tcagcttcat gaagacgctc gatccgttcg atctcgttct gctcatgtcg gatatcaata
     7141 tgccaggaat gacgggcctc gaactgctga aagcgacaaa ggaaaacttt ccgggcctga
     7201 aagtcgtcat gatcactgca tatagtgatg cgcgcaacag gctggccgcg gaagaaggcg
     7261 gagcggtggc gttcctgacg aaaccgctga atttcgaaga gctgaggaac atattgttta
     7321 acccaaacga aaacagctga tggcagcgaa aatacttgtc gtcgacgatg agagcgatct
     7381 cgagctgctc atccgccaga agttccggca cgccatcaaa tcaggcgagc tgctgttcga
     7441 ttttgcgctc aacggtgtgg aagcgttgaa aaagctggaa ggcgacaggg aatacagcat
     7501 ggtgatcacc gacatcaaca tgccggagat ggacggactg accctgctgg gaaagatcgg
     7561 tgaaatggac cgcccgctca aagcggtcgt catttccgct tacggcgata tgggaaatat
     7621 ccgcaccgcg atgaaccggg gcgcattcga tttcatcacg aaaccgatcg acctcaagga
     7681 tctcgaggtg accattgcga aaacgctgcg cgaaaccgac gcgatacgcc agggtctcgc
     7741 ggcgaaagaa aacctcggga aagcgttgtt cgaaaaagac aaagccgaag aggccaaacg
     7801 gttcgagcag caattcctcg ccaacatgag ccacgagatc cgcacaccga tgaacgccgt
     7861 gatcgggatg acgaacctcg tgctgaaaac agagctgaac gaacgccagc ggaaatacgt
     7921 cgacgccatc cgcgtttctt cggaaaacct gctgggcatc atcaacgata tcctcgacat
     7981 ctcgaagatc gaagcgggca agatcgatct cgaaagcatt ccgttcagcg tgaaggatgt
     8041 gctcggcaac gtttacaccg tgctgcacct gaaagcggag gaaaaaagtc tttcgctcgt
     8101 gatggaaacc ggtgaaacag ttccgcaggc gcttctcggc
//
LOCUS       DASTSF010000181         3995 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1898444,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000181 DASTSF010000000
VERSION     DASTSF010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3995
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1898444"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..524
                     /locus_tag="VFU15_05300"
     CDS             <1..524
                     /locus_tag="VFU15_05300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717223.1"
                     /translation="ARADKQRQKSLDRIAKDREKKDKQRAKAKAKELNQYDDCDSTKI
                     VRTSKDTAILVKYIRIYRTLGGACYILHLQRMAKLEKTADGFYSVQMKESSDPRAPAD
                     WQTYLYCPIGKPNGLVALTSPAGDTVQTCQYQDEKKNGDMIWYKKGKGAIHQEIYTND
                     VRQMNMLDTLMNH"
     gene            complement(608..1285)
                     /locus_tag="VFU15_05305"
     CDS             complement(608..1285)
                     /locus_tag="VFU15_05305"
                     /inference="COORDINATES: protein motif:HMM:NF014591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family nucleotide pyrophosphatase"
                     /protein_id="HEU4717224.1"
                     /translation="MLPENFHVILASKSPRRQFLLHGIGIDFEQKTMEVDESFPDHLH
                     DEEIPLYLCRKKAEAFQHVLGEKTLIITADTIVWINGHVLNKPADEEEARIMLRELSG
                     NRHEVYTGVCLKTQKKEVSFSVRTDVLFNELSEEEITFYIRAYKPFDKAGSYGAQECL
                     PPGMNPCSGEEIAFLDRIGKSRLAVPPVSPGVKQRSIHAIRKIEGSYFNVMGLPVMEL
                     YGELEAF"
     gene            complement(1275..2285)
                     /locus_tag="VFU15_05310"
     CDS             complement(1275..2285)
                     /locus_tag="VFU15_05310"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglycerol-phosphate
                     geranylgeranyltransferase"
                     /protein_id="HEU4717225.1"
                     /translation="MISFFKLIRLPNLLIMAFTQYMVRWCVVLPVLALKRSDLQFSLL
                     ESKMSELDFFLLVLSTVMIGAAGYIINDYFDVRIDEVNRPKTNVVGKGIKRRVAMGAH
                     MVINILGAGIGIWLSWKYGMFRVGSFIYITAPALLWFYSTNLKRQFLAGNLVIALLSG
                     LVPMMVILFDLPNISREFPDLVQSGLLNLSDIVHVTFMVAVFAFSVSFLREMIKDTED
                     YEGDLAYGCKTAPIVLGIARTKWIMAALAAFVIGLLGWLELKQFKGVLLPPEFSLALY
                     LYFFILLQLPLAFLVYRLLKAKTKQDWKFASLLVKIVMVAGISYLFLYAHGINSLIEN
                     AS"
     gene            2394..2822
                     /locus_tag="VFU15_05315"
     CDS             2394..2822
                     /locus_tag="VFU15_05315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717226.1"
                     /translation="MKKMLIALLVALLAGSANAQTVISNWSNDEKIFELNDGVLSKWS
                     NDEKIYELKDGVIMNWSNDEKLYEIKDNVLCKWSTDEKLYEYKDGVISRWSNDEKLYE
                     VKDGVIMKWSNDEKLYEIKGDYSVDELFMIMVGLGLLSDE"
     gene            complement(2885..3385)
                     /locus_tag="VFU15_05320"
     CDS             complement(2885..3385)
                     /locus_tag="VFU15_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006987206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonate 8-phosphate
                     phosphatase"
                     /protein_id="HEU4717227.1"
                     /translation="MTENFKQKLNRIRAFVFDIDGVLTDGKITFHADNTTSRSLNAKD
                     GYALQRALRAGFFVAVISYAKDEVLRERLKNIGLTEVYLGSTDKEETLKEFAAVYSLG
                     YDEIAFMGDDVPDILAMKRCGIAACPYDAAPDIREISIYISPKKGGEGCVRDIIEQAL
                     RLSGKW"
     gene            complement(3382..>3995)
                     /locus_tag="VFU15_05325"
     CDS             complement(3382..>3995)
                     /locus_tag="VFU15_05325"
                     /inference="COORDINATES: protein motif:HMM:NF022188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Rossmann-like and DUF2520 domain-containing
                     protein"
                     /protein_id="HEU4717228.1"
                     /translation="INRDADLVLIAVNDDAIAGVAKKLEHFSGTVVHTSGSVGMDVLK
                     RSARRGVLYPLQTVSGNRPLRLKNIPLCVEASGKETKKLLHTIAGTLSHDVHDINSGE
                     RRKLHLAAVFVNNFSNHLYTLAEEYLRTNRLSFNLLRPLILETAKKAVENSPAKSQTG
                     PAARNDTRTISAHLALLKKNKQMKKIYTDLTASILERSRQKRS"
BASE COUNT          961 a         1037 c         1097 g          900 t
ORIGIN      
        1 gcgcacgggc ggacaagcag cggcagaaga gcctcgaccg tattgcgaaa gaccgggaga
       61 aaaaagataa gcagcgggcg aaggcgaaag cgaaagaact caaccagtac gacgattgcg
      121 acagcacgaa aatcgtgcgc acttctaagg atacagccat tcttgtaaag tacatccgta
      181 tctaccgcac gctgggcggc gcctgctaca ttcttcacct gcagcgcatg gcgaagctgg
      241 aaaaaacagc ggacggattc tattccgtgc agatgaagga aagcagtgat ccccgcgcac
      301 ccgccgactg gcagacgtat ctctattgcc cgatcgggaa gccgaatggt cttgttgcgc
      361 ttacttcacc cgccggcgac accgtgcaaa cctgccagta ccaggacgaa aaaaagaacg
      421 gcgatatgat ctggtacaaa aaagggaaag gcgccattca ccaggaaatt tatacgaatg
      481 acgtgcggca aatgaatatg ctcgatacgc tgatgaacca ctgagccttt tgtttttcgc
      541 ctggtccacg gctaaggtcg catgaatgaa gcttcgcgtt cttttggtca ataaaacagg
      601 cgtactgtca gaacgcctcc agttccccgt acaattccat cacgggaagt cccatcacgt
      661 tgaaatagga accttcgatc ttccggatcg cgtgaatgct gcgttgtttt accccgggcg
      721 acacaggcgg aaccgccagc cggcttttgc cgatccggtc caggaaagcg atctcctcac
      781 cggagcaggg attcattccg ggcggaagac attcctgcgc gccgtacgat cccgctttgt
      841 cgaaaggttt gtaagcgcgg atataaaaag tgatctcttc ttcggaaagc tcattgaaaa
      901 gcacgtcggt gcggacagaa aaggaaacct cttttttttg tgttttcagg cagacacccg
      961 tatacacttc atgcctgttc cccgaaagtt cccgcagcat gatgcgcgct tcttcctcgt
     1021 ccgccggttt gttgagcacg tgaccgttta tccacacaat agtatccgcg gttatgatca
     1081 gtgttttttc accgagcaca tgctggaacg cttccgcttt tttgcggcac aggtaaagcg
     1141 gtatctcttc atcgtgcagg tgatccggga acgactcatc cacttccatc gtcttctgct
     1201 cgaaatcaat gccgatgccg tgaagcagga attgcctccg cggcgatttg gaggcaagga
     1261 tgacgtgaaa attctcagga agcattttcg atcaggctgt tgatgccgtg cgcgtaaagg
     1321 aaaaggtagc tgatgcccgc caccatgacg atcttcacca gcaggctcgc gaatttccag
     1381 tcctgcttcg ttttcgcttt cagcaggcgg taaacgagga acgccagcgg cagctgcagg
     1441 agaatgaaaa agtagaggta aagcgccagc gaaaattccg gaggcagcag gactcctttg
     1501 aactgtttca gctccagcca gccgagcagg ccgatcacga aggcggcaag cgcagccatg
     1561 atccatttcg tgcgggctat gccgagaacg atgggcgccg ttttgcatcc gtacgcaagg
     1621 tcgccttcgt agtcttccgt gtctttgatc atttcccgaa ggaaagaaac ggagaacgca
     1681 aatacggcca ccatgaacgt cacgtgcacg atgtcgctga ggttgagcag gccgctttgt
     1741 acgagatcag ggaattccct ggaaatgttc ggcaggtcga aaaggatgac catcatcggc
     1801 accaggcccg aaagaagtgc gatgacgaga ttgccggcga gaaattgccg cttcaggttc
     1861 gtggaataga accagagcag ggcgggagcg gtgatataaa tgaacgaacc cacgcggaac
     1921 atgccgtatt tccacgacag ccagatgccg atgcccgcgc cgaggatgtt gatcaccatg
     1981 tgcgctccca tggccacacg gcgcttgatg cctttcccga caacgttcgt tttagggcgg
     2041 ttcacctcgt cgatgcggac gtcgaaataa tcattgatga tgtacccggc cgcgccgatc
     2101 attacagtgg acagcacgag caggaaaaaa tcgagctcgg acatcttcga ttccagcagg
     2161 gaaaactgca ggtccgatct tttcagcgcc agcaccggca gcaccacgca ccagcgcacc
     2221 atgtactgcg tgaaagccat gatcagtagg ttgggaaggc gtatcagctt gaaaaaggag
     2281 atcatcgggg cagtaaaagt aagtaaagtt gggccttgtt cgtgctgctg cagcggcggg
     2341 cgccgtgctt gcggatcgtg cattcttttc caatctttgg taaaacgaat cccatgaaaa
     2401 agatgctgat cgcgctcctg gtggcgcttc ttgccggttc cgcaaatgcg cagactgtta
     2461 tttccaactg gtcgaacgat gaaaagatat tcgagctgaa cgacggcgtt ctcagcaaat
     2521 ggtccaacga tgagaaaatt tacgagctga aagacggcgt gatcatgaac tggtcgaacg
     2581 acgaaaagct gtatgaaata aaggataatg tgctctgcaa atggtcgacc gacgaaaagc
     2641 tgtacgaata caaggacggc gtgatcagcc gctggtcaaa cgatgaaaag ctttacgaag
     2701 taaaggacgg ggtgatcatg aaatggtcaa acgatgaaaa gctctacgag atcaaaggcg
     2761 attattcggt ggatgaattg ttcatgatca tggtcggcct cgggctgctt tcggatgaat
     2821 gatttcagcg agccgcccaa ccccggaacc gctcagcttt tcaactttcc aactttgaac
     2881 agcattacca tttcccgctc agccttaacg cctgctcgat aatatcccgc acgcagcctt
     2941 caccgccttt tttcggagaa atgtaaatgg aaatttcgcg gatgtcgggt gcggcgtcgt
     3001 acgggcaggc cgcaattccg cagcgtttca tcgccagtat gtcaggtaca tcgtcgccca
     3061 tgaacgcgat ctcatcgtat ccgagcgaat acacagccgc aaattctttc agcgtttctt
     3121 ccttgtcggt gctgccgagg taaacttccg tcaggccgat gttcttcagc cgttcacgca
     3181 ggacctcatc tttcgcgtac gagatcaccg caacgaaaaa accggcacgc agtgcgcgct
     3241 gcagcgcata accgtctttg gcgttcaggc tgcgcgacgt cgtattgtcg gcatggaacg
     3301 tgatcttccc gtcggtcagc acgccgtcga tgtcgaatac gaatgcgcgg atgcggttca
     3361 gcttctgctt gaaattttcg gtcatgatct cttttgtcgg gagcgttcga gaatggaagc
     3421 ggtgaggtcg gtgtagatct ttttcatctg cttgttcttt ttcagcaggg caaggtgcgc
     3481 gctgatcgtc cgggtgtcgt ttcgcgcggc aggaccggtc tgcgatttcg ccggggaatt
     3541 ttccacggct ttcttcgcgg tttcgaggat aagggggcgg agcaggttga agctgagccg
     3601 gttcgtccgc aggtattctt cagccagcgt gtacaggtga ttggaaaaat tgttcacgaa
     3661 cacagcggca aggtgcagct ttctgcgttc tccggaattg atgtcgtgca catcatgtga
     3721 aagcgttccg gcgatcgtgt gcagcagctt ttttgtttcc ttaccggaag cttccacgca
     3781 gagcggaata tttttcaggc gaagcgggcg gtttcccgaa acagtttgca gcgggtaaag
     3841 cacgccgcgc cgcgcggatc ttttcagcac atccattccc acgctgccgg aagtatggac
     3901 aaccgttccg ctgaaatgtt ccagcttttt cgcgacgccg gcaatggcgt cgtcgttcac
     3961 cgcgatgaga acaagatccg catcacggtt tattt
//
LOCUS       DASTSF010000182         4714 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_49754,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000182 DASTSF010000000
VERSION     DASTSF010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4714
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49754"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            74..1603
                     /locus_tag="VFU15_05330"
     CDS             74..1603
                     /locus_tag="VFU15_05330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717229.1"
                     /translation="MKPAPDLFNLVHSLDKFEKRYVTLFLSSGFYRNNKSSLALFRAI
                     SRQKEFDETALKKQLGRSVTARFSAEKNKLFDLILESMFFLYRDSSPERKIVRERLRA
                     WFLFQKGLKQAGWKYFHRAVRLAESYEITPQLAMLAYQQNQEVRLSAAEDALFSSAEF
                     HRRDERLTRGLSEDLVLHTLFTEMIQVQKHHGEYTPAAEKEFRAILRHPLLKPSRKLI
                     SFSAQMSRIEIRSLYYSMRGDHDKAYECFVELIAAMETSETYIHRMYVRYVNALSNQL
                     MHAVLSHRYEVVPGLVEKTMEANRGILDYFSYDIVYNEIIGIRLFELVAYKNRADTAK
                     GPAMMEYLEKMFARYRPQMRDTLVVGYLFLFGVYHFYLGDRKKALLHLNDLVDSTGME
                     AGLNFQCMARLVRLVIFYELGDAELVNSQCQSVQRLLRKHGRLGAFEQALLVFLRKTV
                     TQSGRPATQKLLSLAHRLAPGSYSYGAWSDFEFGSWLKSRIEKRPLRELIAARLKGKG
                     K"
     gene            1862..3703
                     /locus_tag="VFU15_05335"
     CDS             1862..3703
                     /locus_tag="VFU15_05335"
                     /inference="COORDINATES: protein motif:HMM:NF015926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF349 domain-containing protein"
                     /protein_id="HEU4717230.1"
                     /translation="MENNVNETSQEEQKAEGSSEASGMNELTAEHETEANQDELHPEE
                     DFSSYGKNELLQKMEEAAGSPDPEHVKGTVNKLKEVFRELVREEMEAKRRAWETSKED
                     EHDVFMPAPDAVAEKFEELLKKYNQKRSELRRQKENEQRKNLALKLQLIDDLKELAES
                     SESMHKAFERLQELQEKWRQIGPVPQANVSELRQNYQHHLDRFYDMVKISRELRELDH
                     KKNQELKNELIAKAEALKDEPSVRRALSQLHELHEQWREIGPAPKEVNDSLWDTFKAA
                     SDKVHDRKQALLEDNKVKQVENLALKTELCVMMDAEAEKTYDSHKAWQDANEKVEALF
                     AEWRKIGHVPKEDEGKAWKRFREARQKFFRSREMFYAKQRDEFKKNLDAKIALCEKAE
                     ALKESKDWKNTALQFRRLQDEWKKIGPVPRKLSDKTWQRFKAAADAFFEGRNKQFAEA
                     DAAMKENVTVRETLIAEAAATVLDGDLKVSRDIIASFQKKWGEMPQVPRSETERLGRS
                     WKEAMDKLFVQLKEKGGDENMVQRMRYDQLKQTEKGRDQIYRERMAIQDKIKKLQNEI
                     NTLETNLGFFSKSKGGQSLVADYQAKVDSAREEVVRLKAQLKMIPRE"
     gene            3758..4312
                     /locus_tag="VFU15_05340"
     CDS             3758..4312
                     /locus_tag="VFU15_05340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717231.1"
                     /translation="MPLFIDVSQKKISIGDKYTIAVNGQPMYKAAAKLFKLFPSISVS
                     PLNGPETPIITVEQKMGWLKPNYQFTINGATYSLETISWWKRRFAITAGANKLEIVGH
                     RGRKVSIFINGKQVAWFDNEAVTFFSGDNYHVTANADAPWEWIVAVVLFWDSFYNRQQ
                     KGAINFKMGAMTQTQPFDASWQAS"
     gene            complement(4402..>4714)
                     /locus_tag="VFU15_05345"
     CDS             complement(4402..>4714)
                     /locus_tag="VFU15_05345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717232.1"
                     /translation="RNNTVYVLEGMKGDEKFAYEIPSMLVVECESEGQPVTRFAASRR
                     LVGDTKKSVDKKGNIHFFIFLKGKNKMVNPVNGVYISAQDFPFDLPVPEEEEITGDEE
                     E"
BASE COUNT         1244 a         1215 c         1262 g          993 t
ORIGIN      
        1 gttttcattg cggatggtat ccgctgaaac cctgtaatga cgcggcttta caagtatcga
       61 tcttactctt tccatgaaac ctgcacccga tcttttcaat ctcgttcact ccctcgacaa
      121 attcgaaaaa cgatacgtga cgcttttcct ttcgtccggt ttttacagga acaacaagag
      181 cagtcttgcg cttttcagag ccatcagcag gcagaaggag tttgacgaaa cagcgctgaa
      241 aaaacaactg ggccggtcgg tgaccgcacg attttcagcc gagaaaaata agctcttcga
      301 tctcatcctc gaaagcatgt tctttcttta tcgcgactct tctcccgaac ggaagatcgt
      361 gcgcgaacgg ctgagggcgt ggttcctttt ccagaaagga ttgaaacagg ccggctggaa
      421 atattttcac cgtgcggtac ggttggcaga gtcgtatgaa ataacgcccc agctggcgat
      481 gcttgcctac cagcaaaacc aggaggtgcg gctttccgct gcggaagacg cccttttcag
      541 cagtgcggaa tttcaccggc gggacgaacg cctcacgcgc gggctcagcg aggatctcgt
      601 tctgcatacg cttttcacgg aaatgatcca ggtgcagaag catcacgggg aatatactcc
      661 cgcggcggaa aaggaattcc gtgcgatcct gcggcacccg ttgctgaaac cttcacggaa
      721 actgatctct ttttcggcgc agatgagccg gatcgagatc cgttcacttt attattcgat
      781 gagaggcgat cacgacaagg cgtatgaatg ttttgtcgag ctgatcgctg cgatggaaac
      841 atccgaaacg tatatccacc ggatgtatgt gcgttatgtc aatgcacttt ccaaccagct
      901 catgcatgct gtactttccc atcgctacga ggtcgttccc ggcctggtgg aaaaaacgat
      961 ggaagccaac cgcgggatac tggattattt cagctacgac attgtttaca acgagatcat
     1021 cggcatacgt ttgttcgagc ttgtcgcgta caagaaccgg gcggatacgg cgaaaggccc
     1081 ggcaatgatg gaatacctgg aaaagatgtt tgcccgctac cgcccgcaga tgcgggacac
     1141 gctcgtggtc ggttacctgt ttttgttcgg cgtatatcat ttctacctcg gcgaccggaa
     1201 aaaagcattg ctgcatctca atgatcttgt cgattcaacc gggatggaag ccggtctgaa
     1261 tttccagtgc atggcccgcc tggtgcgcct ggtcattttt tatgaactgg gtgatgcgga
     1321 gctcgtgaac agccagtgcc agtcggttca gcgattgctg cgcaagcatg gccggcttgg
     1381 tgcgtttgaa caggccctgc tggtgtttct ccggaaaacg gtaacgcaga gtggccggcc
     1441 cgccacgcag aaactgctgt cgcttgcaca tcgcctggcg cccggaagct acagctacgg
     1501 cgcctggagc gattttgaat tcgggtcctg gctgaaaagc aggattgaaa aacgcccttt
     1561 gcgtgaactg atcgctgcgc ggctgaaggg caaaggaaaa taatcccgcg ttttcttccg
     1621 tagctgccgt gctgcgcgtt ttatcggtga aaaatcatca ttatcatttg gtaaataatt
     1681 ttccgggaat atcctgatgc ggcacaatac gaagaatttc gcagacaggc gatacggcct
     1741 gtacgcgtca ttaccgatat ttttttccct ttccgattcg aaatactttt tcctactttt
     1801 gaagtaaaca ataaatgcac gccgaccttg tcacagacaa ggtttttcat tcttacgtca
     1861 catggagaat aacgtgaacg agaccagcca ggaagaacaa aaagccgaag gctcaagcga
     1921 agcttccggc atgaacgagc ttacagcgga acacgaaacg gaagcgaatc aggatgagct
     1981 tcacccggaa gaagattttt cttcgtacgg gaaaaacgag ctgctccaga agatggagga
     2041 agcggcggga agtccggatc cggagcacgt gaagggaacc gtgaacaagc tcaaggaagt
     2101 tttccgcgag ctcgtccgcg aagagatgga agcgaaacgc agggcctggg aaacatcgaa
     2161 agaggacgag cacgatgtgt tcatgcccgc acccgatgca gtggcggaaa aattcgagga
     2221 acttcttaaa aaatacaacc agaaacgcag cgagctccgt cgccagaagg agaacgagca
     2281 gcggaaaaac ctcgcgctca agctgcagct gatcgatgat ctgaaagaac tcgccgaatc
     2341 gagcgagagc atgcacaaag cgttcgaacg tttgcaggag ctccaggaaa aatggaggca
     2401 gatcggtccc gtgccgcagg cgaacgtatc cgagctgcgc cagaattacc agcatcacct
     2461 cgatcgtttc tacgacatgg tgaagatcag ccgcgagctg cgcgagctgg atcacaagaa
     2521 gaatcaggag ctgaagaacg agctcatcgc caaagcggaa gccctgaagg acgaaccttc
     2581 cgtgcgccgc gcgctttcac agctgcacga attgcacgag cagtggcgcg agatcggtcc
     2641 ggcccccaaa gaagtgaacg attccctgtg ggatacgttc aaggccgctt ccgacaaagt
     2701 gcacgaccgc aagcaggcgc tgctcgagga taataaagtg aagcaggtcg agaacctggc
     2761 gcttaagaca gagctgtgcg tgatgatgga tgccgaagcg gaaaaaacgt acgattcgca
     2821 caaggcatgg caggacgcca atgaaaaagt ggaggcgctc tttgccgaat ggcggaagat
     2881 cggtcacgtt ccgaaagaag atgaagggaa ggcctggaaa cgttttcgcg aagcccgcca
     2941 gaaatttttc cgcagccgcg aaatgttcta tgcaaagcag cgcgatgaat tcaaaaagaa
     3001 cctcgacgct aagatcgcgc tctgcgaaaa agccgaagcg ctgaaagaaa gcaaagactg
     3061 gaagaatacc gcgctgcagt tccgcaggct gcaggacgag tggaaaaaga taggtcctgt
     3121 gccgcgcaag ctctccgata aaacatggca gcgcttcaaa gccgccgcgg acgctttttt
     3181 tgaaggccgg aataaacagt tcgcggaggc cgatgccgcc atgaaggaga acgtgaccgt
     3241 ccgtgaaacg ctcattgcgg aagcagcggc caccgtgctc gacggcgacc tcaaagtttc
     3301 acgcgacatc atcgcatcgt tccagaaaaa atggggcgaa atgccgcagg tgccgagaag
     3361 cgaaaccgaa cgtctcggcc gttcctggaa agaagcgatg gacaaattat tcgttcagct
     3421 gaaagaaaaa ggcggggacg aaaacatggt gcagcgcatg cgctacgacc agctgaagca
     3481 gaccgaaaag ggtcgcgacc agatctaccg ggaacggatg gccatacagg acaagatcaa
     3541 aaagctccag aacgagatca acacgctcga aaccaatctc ggctttttca gcaaatccaa
     3601 aggcgggcaa tcgcttgtcg ccgattacca ggcgaaagtg gattccgcac gcgaggaagt
     3661 ggtgcgcctc aaagcgcagc tgaaaatgat tccccgggaa taactaattt tcaaccgatc
     3721 aattcacccg tttcactttc aaaaccgaaa acaaaccatg cctcttttca tcgacgtcag
     3781 ccagaaaaag atctccatcg gagacaagta cacgatcgct gtcaacggac agcccatgta
     3841 taaagcagcg gccaagctgt tcaaactttt cccgtccatt tccgtttctc ctctgaacgg
     3901 acctgaaaca ccgatcatca cggtggagca gaagatggga tggctcaagc cgaactacca
     3961 gttcaccatc aacggcgcca cctacagtct cgaaacgatc tcgtggtgga aacgccgctt
     4021 cgccatcacc gccggcgcca acaaactgga gatcgtgggt caccgcggac gcaaggtttc
     4081 cattttcatc aatgggaaac aggtggcctg gttcgacaac gaagcggtta ctttcttcag
     4141 cggcgacaat taccacgtga cggcgaatgc ggacgcacct tgggaatgga tcgttgcagt
     4201 cgttctcttc tgggattcat tctataaccg ccagcagaaa ggcgcgatca atttcaagat
     4261 gggagcaatg acgcagacgc agccgttcga cgcttcatgg caggccagct gatctgtttc
     4321 ttaaacgata attaatgggt cccgggaaat ttctcccggg attttttctg aacagaagaa
     4381 cgttaaacag caggatgcag gtcattcttc ctcgtctccc gtaatttcct cctcttccgg
     4441 aacgggaagg tcgaacggga aatcctgtgc ggaaatgtaa acgccgttca ccgggttgac
     4501 cattttgttt tttcctttca ggaaaatgaa aaaatggatg ttgccttttt tgtcgactga
     4561 ttttttcgtg tccccgacca gccgtctcga agcggcgaaa cgcgtcacgg gttgtccttc
     4621 gctttcgcat tccacgacga gcatggaagg tatctcgtag gcgaacttct catcgccctt
     4681 cattccttcg agtacataaa ccgtgttgtt ccgg
//
LOCUS       DASTSF010000183         3220 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_435447,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000183 DASTSF010000000
VERSION     DASTSF010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3220
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_435447"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /locus_tag="VFU15_05350"
     CDS             complement(<1..239)
                     /locus_tag="VFU15_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011966691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEU4717233.1"
                     /translation="MSDLKYNRRGVSASKEDVHNAIKNIDKGLFPNAFCKIIPDHLSG
                     DEEYCLVMHADGAGTKSSLAYMYWKETGDMSVWKG"
     gene            complement(304..2622)
                     /locus_tag="VFU15_05355"
     CDS             complement(304..2622)
                     /locus_tag="VFU15_05355"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEU4717234.1"
                     /translation="MKLKKVLSVVVVFLLAFTYRAYAQKNYIRDADRAFESQQYFNAS
                     ELYKKGMAKIKNKQEKARITYQIAECYRYMNDWKQAESWYGKAIKAKYTNDKMYLYYA
                     EAKKINKKYDEAITAFQDYQKMVPSDPAGENGIKSSELAQKWIDNPTRWVVENMAQIN
                     SKDYDFCPTFSDKKHTSLIFTSKREGQTGSKIDPVSGTLYSDLFETKVDKNGKWSSPS
                     TIQGSVNTAIANEGASCVNKKGDKIYFTRCDQQKKKWITCKIYVSAHKGNSWDVPELI
                     DFGLDAATLDSFNFRHPTVSADDQVMVFSSDMTGCMGGIHSDLWMSTYDKKSKKWQKP
                     VNLGPTINTEGREGFPYISESGDLYYSSDGLLGMGGLDIFKAPKTDAKDWKWGTPENL
                     MYPMNSPGDDFGIVFDGKKEKGYLTSNREGTKGVDDIWSFYLPPLVFHLNGVVTDCKY
                     GPSVTVQNATVRLVGSDGSALEVQTDATGKYHFDLLPEVSYIVTVFSDGKATSTKANG
                     YLNLPDKDKGKFTTVGEMQSKDFTLDFCLVPQEDEIRFPAVLYDLGKATLRPESKDSL
                     NFLYQTLIDNPTIIIEIGSHTDSRGSNASNQKLSQARAQSCVDYLVNEKHIPKDRLVA
                     HGYGEERPLRLPDGTVLTDKYIMSKKTKEEQEALHQLNRRTAFKVLSWDYVDPNAPKD
                     QNQRKIVKPKVMSGYFDDTGDTTGADIQDAPVDGAGNAPAGGNNQAGTPPAGGTPPAN
                     TPPGNNPPKAGTNPPANNPPKAGTQQPKPKNS"
     gene            complement(2837..>3220)
                     /locus_tag="VFU15_05360"
     CDS             complement(2837..>3220)
                     /locus_tag="VFU15_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013665373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEU4717235.1"
                     /translation="RGSSSANLTLSQQRAQACVDYLVNEKGIPRARLVAKGWGEGKPL
                     RLPDGTVLTDNYINSRKTVAEREALHQLNRRTTFQVLSNDYHDPNAKQAPAQKVEVKK
                     GYFDNTGDEDDDDTDDSGGNAPQKP"
BASE COUNT          690 a          796 c          808 g          926 t
ORIGIN      
        1 attcctttcc acacgctcat gtcgcccgtt tctttccagt acatgtacgc gagcgaagat
       61 tttgttccgg caccatcggc gtgcatgaca agacaatact cttcatctcc cgagagatga
      121 tcgggaatga tcttgcagaa tgcgttcggg aaaagtcctt tgtcaatatt ctttattgca
      181 ttatggacat cctccttgga agcagaaacc ccgcgccggt tatatttcag atcactcata
      241 tcggtaaaca aagaatcccg cattttcacg cgggattcaa agttagctat tctgaaaaag
      301 ccatcaggaa ttttttggct tcggctgttg cgttcctgct ttgggaggat tgttcgccgg
      361 aggatttgtt cctgccttcg gcgggttatt tcccggagga gtattagccg gaggagttcc
      421 gcctgccggg ggagttcctg cctggttgtt tccgccggca ggtgcgtttc cggctccatc
      481 aaccggtgcg tcctgtatat cagcgcccgt cgtatcaccc gtatcatcga aataaccgct
      541 cattactttc ggcttaacga tcttacgctg gttctgatct ttcggtgcat tgggatcaac
      601 ataatcccag ctgaggacct tgaatgcggt acggcggttc agctggtgaa gcgcttcctg
      661 ctcttccttg gtcttcttgc tcatgatgta cttgtccgta agaactgttc cgtcaggcag
      721 gcgcagcggg cgttcttcgc cgtatccatg cgctacaaga cgatctttcg gaatgtgttt
      781 ttcattgacg aggtaatcaa cgcacgactg cgcacgggcc tgggacagct tctggttgga
      841 agcgttcgaa ccgcgggagt cggtgtgtga accgatctcg atgatgatcg tcgggttgtc
      901 aataagtgtc tggtaaagga agttcagcga atcttttgat tccgggcgca gcgtagcttt
      961 tccgaggtca taaagcaccg ccgggaagcg gatctcgtct tcctgcggaa cgaggcagaa
     1021 atcaagcgta aagtctttcg actgcatttc gccgaccgtt gtgaattttc ctttgtcctt
     1081 gtcaggaagg ttgaggtagc cgttcgcttt tgtgctcgtc gcttttccgt cggagaacac
     1141 cgttacgatg tacgaaacct caggaagaag atcgaagtgg tatttaccgg ttgcatccgt
     1201 ctgcacttca agcgcagatc cgtcggaacc aacaagacga actgttgcat tctgtacagt
     1261 aaccgaagga ccatatttac agtcggtaac aacaccattc aggtggaata ccagcggcgg
     1321 aaggtagaac gaccagatgt catcgacacc tttcgtgcct tcgcggtttg aggtaaggta
     1381 tcctttctct ttcttaccgt cgaacacgat accgaagtcg tctccgggcg agttcatcgg
     1441 gtacataagg ttttcagggg ttccccattt ccagtctttc gcatcggttt tcggagcttt
     1501 gaaaatatca agtccgccca tgccgaggag tccatccgaa gaataataca gatcgccgct
     1561 ctcagagata taggggaatc cttcgcggcc ttccgtgttg atcgtcggac cgagattcac
     1621 cggcttctgc cattttttgg atttcttgtc atacgtcgac atccagaggt cggagtgaat
     1681 tccgcccatg caacccgtca tgtccgaaga gaaaaccatt acctggtcat ccgcgctcac
     1741 ggtcgggtga cggaagttaa agctgtcgag tgttgctgcg tcaagaccga aatcgatcag
     1801 ttccggaacg tcccaggaat tccctttgtg ggccgaaacg tagattttgc aggtgatcca
     1861 ttttttcttt tgctggtcgc agcgggtaaa atagatcttg tcgccttttt tgttcacaca
     1921 gctggcgcct tcattggcga tcgccgtgtt cacagaaccc tggatggtgg aaggagagct
     1981 ccatttaccg ttcttgtcga ctttcgtttc gaaaaggtcg gaataaagtg tgccggaaac
     2041 agggtcgatc ttgctgccgg tctggccttc gcgcttggag gtgaaaataa gcgaggtgtg
     2101 ttttttatcg ctgaacgtcg ggcagaaatc ataatccttt gaattaatct gcgccatgtt
     2161 ctcgaccacc cagcgtgtag gattatcgat ccacttctgc gccagctcgc tggacttgat
     2221 cccattttca ccggcaggat ccgatggcac cattttctgg taatcctgga acgccgttat
     2281 ggcctcgtcg tattttttat tgattttttt cgcttccgcg taatacaggt acatcttatc
     2341 gttggtgtac ttggctttga tcgcctttcc ataccagctt tccgcctgct tccagtcgtt
     2401 catgtagcga taacattcag cgatctggta agtgatgcgg gctttttcct gcttgttttt
     2461 gattttagcc atgccctttt tgtagagctc gctagcgttg aagtactgct gcgattcaaa
     2521 cgcgcggtcg gcgtcgcgta tgtagttttt ctgtgcatac gcccggtaag taaaggcaag
     2581 cagaaatact acaacaacag atagtacctt tttcagtttc ataggaaata aggttttttt
     2641 agtgtttcag ttttgacgtg gctgctaagt taattaaaac ccgaaaacgg cgaaattaaa
     2701 tttccttttt tggccagagg aagcagtcct tcccaattca tataaccatc ccgaacaaaa
     2761 atggtacagg aaatttcccg taccattttt aaaacactga atatcagaat attattttta
     2821 ctttgagctt tgaaaatcac ggcttttgag gtgcgtttcc accggaatcg tccgtatcgt
     2881 cgtcatcctc atcaccggta ttatcgaaat aacctttttt cacctccact ttctgtgcgg
     2941 gcgcctgctt tgcattggga tcatgataat cgttgctcag cacctggaag gtcgtgcgcc
     3001 ggttcagctg gtgaagcgct tcgcgttcgg ccacggtttt cctgctgttg atgtagttgt
     3061 cggtaagaac agttccatca ggaaggcgca gcggcttgcc ttccccccag cctttcgcca
     3121 cgagacgtgc ccgtggaatt cctttttcat tcacgagata gtccacacag gcctgggcgc
     3181 gctgctgcga aagcgtgaga ttggcggagg aagatccgcg
//
LOCUS       DASTSF010000184         5203 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_786314,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000184 DASTSF010000000
VERSION     DASTSF010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5203
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_786314"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..363)
                     /locus_tag="VFU15_05365"
     CDS             complement(<1..363)
                     /locus_tag="VFU15_05365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717236.1"
                     /translation="MRAFFFLSLVLFGCANAPETPAVKPGATDQSQKTFVPELSDSIE
                     GDFDGDGKKEWAVLAMTKDGSRNLPANPDDPAKFEVLFRNGKIKPLDAGCCDLRLVKE
                     DDLDDDGGDEISVFGNEGN"
     gene            complement(379..1617)
                     /locus_tag="VFU15_05370"
     CDS             complement(379..1617)
                     /locus_tag="VFU15_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018479303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HEU4717237.1"
                     /translation="MILNYVWVLFILAAFVIGLVRLVFFGDTEIFPAMVNGTFSSAKT
                     GFEVALGLTGALTMWMGILRIGEKAGAIRFLGRIISPFFSRLFPAIPKDHPALGHIML
                     NFSANMLGIGNAATPLGLQAMKSMQEINTEKETASNPMIMFLALNTAGFTLVPVSILA
                     LRAQAGAADPSCVFIPIMIASFFASFAGMIVVAIRQKINLFDKVLLAWFAGITAFVAG
                     MIALFLNMSPEKRAVVSNVEGNFILFAVIALFVTGGVIKKLNVFSIFIDGAKEGFSTA
                     VRIIPYLVAMLVAIGVFRSCGALDYLLAGFAWCFHHIGVNTDFIPGLPVAFMKPLSGS
                     GSQSLVADILNNKMYGPDSFVGKMTSVMYASGDTTFYIVALYFGAVGIQKTRYSVTAG
                     LIADAAGIVAAIVLSYLFFH"
     gene            1745..2158
                     /locus_tag="VFU15_05375"
     CDS             1745..2158
                     /locus_tag="VFU15_05375"
                     /inference="COORDINATES: protein motif:HMM:NF023390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS/SEC14 domain-containing protein"
                     /protein_id="HEU4717238.1"
                     /translation="MTLPIVKNNLRLYEVKAEIYSDELGFARLDFFPGTEIDEKDARE
                     ILRISLQTRTGDERFYVLIDLENLERVNDKARDFLAGDEMLDFLAAIALVAKTPAMKL
                     VGNFFIRYHKPRLPVRLFSEIEPARKWLSGVRGSS"
     gene            complement(2153..3154)
                     /locus_tag="VFU15_05380"
     CDS             complement(2153..3154)
                     /locus_tag="VFU15_05380"
                     /inference="COORDINATES: protein motif:HMM:NF022779.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3137 domain-containing protein"
                     /protein_id="HEU4717239.1"
                     /translation="MAPGGKYSSIISPEEQQLLDRLDRQRILVRRKPMLWILSSALFV
                     SVMVVTGSIIENKKYENDAWPYVVMLVFTLFFFGCIAWLTFKFPERGEISKQYKRFVL
                     QSFAPKILPGWVFSRSHKLKNEDIRKSGLFKDKANRMAREDYLFGPAGKVVAEVYQIT
                     LQVEPSSNKEQNGGVILKKETPTTFFFGFFYRVHCPLIFLCDVWVFPKHRKLSGEVDD
                     WAEVTEEKYSGDDVRKPVMTSDAAFDERFSVYSNYPPEALNILNHRRRENLLTIDRMF
                     STSCAFSFTGNKVFIMIGFEKDPLDIDLRKTIGEDLFAGQADELLRMKEVALLASAL"
     gene            complement(3320..4315)
                     /locus_tag="VFU15_05385"
     CDS             complement(3320..4315)
                     /locus_tag="VFU15_05385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717240.1"
                     /translation="MNLETPEPSVAVNLPAQTISTLKKLDRKRTFSEKRFSLWFLAPF
                     LAGIPLVLAFVTDYSWLLVFSIIWIFVVIFGLGAFIHVILPEPTELSRVFKKSILPGL
                     LNENGLTAKYYSSHGLKPDSFMKAGLFRENYSHFERLDCISGKYRNIPFWLYELKVQT
                     SDTMGRGLVFTPRTRVVTTVFYGWVLHVPVRTLHGKVFIIPRREKKNDESDDWVKTTR
                     EYFGDRSDYSPLLFDNPEFNARYIVFSDRADRNSAFTAPVLAFFSRLYDISPNAPAFS
                     FCEDLASMHVGISDDGFDLDPGRPSYPDGTDKASGKLRFFSAVVSGLYSVAGEMN"
     gene            4582..>5203
                     /locus_tag="VFU15_05390"
     CDS             4582..>5203
                     /locus_tag="VFU15_05390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717241.1"
                     /translation="MTEQLSLIPPALRTSRPVHFPKILRDRYNLLVPRQEDGLIVLAL
                     YQKVRAGIVERAFPERIIVETIKTVFEELGHGSSRGEESQKYDETLHRLLRHFLDKNI
                     EEKTYQLSSYAENFCDLLEREVHTAINPSQIQKTFNDLVMLLGLKLENMEDFRHWYHT
                     QFFKHKNDISAQLRALQAQIEDTVDQLNELVKNEVDNFREMLIGCEA"
BASE COUNT         1311 a         1373 c         1395 g         1124 t
ORIGIN      
        1 gttcccttca tttccgaaaa cagagatctc gtcgccgccg tcgtcgtcaa ggtcgtcttc
       61 cttcaccagc cgcaggtcgc agcagccggc gtcgagcggt tttattttcc cgttgcggaa
      121 gaggacttcg aattttgccg gatcatccgg atttgccggc aggttgcggc tgccgtcttt
      181 tgtcatcgcc agcacggccc actctttttt cccgtccccg tcgaaatcgc cttcgatgct
      241 gtcggaaagc tccggtacga acgttttttg cgactggtct gttgcaccgg gttttaccgc
      301 cggcgtttcg ggtgcgttcg cacagccgaa aagcacaagc gaaagaaaga aaaacgcgcg
      361 catttttttg caggaagatc agtggaaaaa caggtagctc agcacgatcg ccgcgacgat
      421 gcctgcagcg tcggcgataa gtcccgctgt aacggaataa cgcgttttct gaatgcccac
      481 cgcgccaaaa taaagcgcga cgatgtagaa cgtcgtgtcg ccggaagcgt acatcaccga
      541 agtcattttc ccgacgaacg aatcggggcc gtacatttta ttgttgagaa tgtcggccac
      601 gaggctttgc gatccgcttc cgctcagcgg cttcatgaat gcgacaggaa gcccgggaat
      661 gaaatccgtg ttcacgccga tgtgatggaa gcaccaggca aatccggcca gcaggtaatc
      721 gagcgcgccg caggagcgga aaacaccaat ggccacgagc atcgccacga ggtacgggat
      781 gatgcgtacg gcggtgctga aaccttcttt cgcgccgtcg atgaaaatgg agaacacgtt
      841 cagctttttg atcacgccgc cggtaacaaa cagcgcgatg accgcaaaca ggatgaagtt
      901 tccttccacg ttcgaaacga cagcgcgttt ttcgggcgac atgttcagga acagcgcgat
      961 catcccggct acgaacgcgg taattcctgc aaaccaggcc agcagcactt tgtcgaagag
     1021 atttattttc tgccggatgg cgacgacgat cattcccgcg aacgacgcga agaacgatgc
     1081 gatcatgatc gggatgaaaa cgcaggaagg atcggccgcc ccggcctgcg cacgcagcgc
     1141 gagaatgctc acggggacaa gcgtgaagcc ggccgtgttc agggcgagga acatgatcat
     1201 cgggttggac gccgtttcct tttccgtgtt gatctcctgc atcgatttca tcgcctgcag
     1261 gccaagcggt gttgcagcat tcccgatgcc gagcatgttg gcggaaaaat tcagcatgat
     1321 gtgccccagc gcaggatgat ctttggggat cgcgggaaac agccggctga aaaaaggaga
     1381 aatgatcctg ccgagaaaac ggatcgcgcc cgctttttca ccgatgcgca ggatacccat
     1441 ccacattgtg agcgcaccgg tgagaccgag agcgacttcg aatcctgtct ttgccgaaga
     1501 aaacgtcccg ttgaccatgg ccggaaaaat ttccgtgtcg ccgaaaaaaa caaggcgcac
     1561 gagcccgatg acgaaagccg caaggataaa caacacccag acgtaattga ggatcatgaa
     1621 gtaaatgtaa cgggccgccg cacgtccttt atgaactttt tatgcggctt ttcaacagct
     1681 gctgttggtt gtgaaagaaa gtatttgctt ttccggggac tggttataac ttgcgcctct
     1741 ttgcatgacc ctccctattg ttaaaaacaa tctccggcta tacgaagtca aggccgagat
     1801 atattccgac gagctcggat ttgcgcgtct cgattttttc cccggaacgg agatcgatga
     1861 aaaagatgcg cgcgaaattc tgcggatctc tttgcagaca cgtaccggcg atgagcgttt
     1921 ttatgtgctc atcgatctcg aaaacctgga acgcgtgaat gacaaagcga gagattttct
     1981 cgccggcgat gaaatgctgg attttctcgc ggcgatcgcg ctggtggcga aaacgccggc
     2041 aatgaagctg gtgggaaatt tttttatccg ctatcacaaa ccacggctcc cggtacggtt
     2101 attttctgag atcgaaccgg cgcggaaatg gctgtcgggt gttcgcggca gctcatagcg
     2161 ctgaggccag cagcgctact tccttcatac gcagcagctc gtcggcctgc ccggcgaaca
     2221 agtcttcacc gatcgttttt ctcaggtcga tgtcgagcgg atctttttca aacccgatca
     2281 tgatgaacac cttgttgccg gtgaacgaga acgcgcagga tgtcgagaac atccggtcga
     2341 tcgtcagcag gttttctcgg cgccggtggt tgagaatgtt cagcgcttcc ggcggataat
     2401 tcgaatacac gctgaaacgc tcatcgaatg ccgcatcgct tgtcattacg ggtttccgta
     2461 catcatcacc ggaatatttt tcttccgtca cctcggccca gtcgtccact tcgccgctga
     2521 gcttccggtg tttcggaaaa acccacacgt cgcataaaaa gatcagcggg caatgcacac
     2581 ggtagaaaaa tccgaagaag aatgtagtcg gcgtttcttt tttgaggatg acgccgccat
     2641 tttgttcttt gttcgacgac ggctctacct gcagcgtgat ctggtaaact tccgcgacca
     2701 cttttcctgc cggaccaaaa agataatctt cgcgcgccat gcggttggcc ttgtctttga
     2761 acagcccgct tttgcggatg tcctcgttct tcagcttgtg tgaacgcgag aacacccagc
     2821 caggaaggat tttcggcgcg aatgattgca gcacgaaacg tttgtactgt tttgaaatct
     2881 ccccgcgctc gggaaacttg aacgtcagcc aggcgatgca cccgaaaaag aaaagcgtga
     2941 aaacgagcat caccacgtac ggccacgcat cattctcata cttcttgttc tcgatgatgg
     3001 aacccgttac gaccatcacg gaaacgaaca gcgcggaaga aaggatccac aacatcggtt
     3061 tccgtcgcac gagtatgcgc tgccggtcga gacgatccag caattgctgt tcttcaggtg
     3121 aaatgatgga agaatatttg cccccgggag ccatggaata aaggtgcaaa aaagagcacg
     3181 cgcttcagca tttatccgga aaaattataa gttgatggaa gaaaacggat agtttggcga
     3241 aaaagaaaag tccacctgaa gattccgcaa ccgggaagat aaatgccgga aagaatattt
     3301 cgggcgccgt ctgcgtgaat taattcattt ccccggcaac ggaataaaga cccgaaacaa
     3361 cggcggaaaa aaagcgcagc ttcccggaag ctttatccgt tccgtcagga taagatggcc
     3421 ggccgggatc gagatcgaag ccgtcgtcgg aaattccgac gtgcatgctg gcgaggtctt
     3481 cgcagaatga aaaagccggc gcattcggag aaatgtcata aagccggctg aagaatgcca
     3541 gcacaggtgc ggtgaaagcg ctgttcctgt cggcgcggtc ggaaaaaacg atgtagcgcg
     3601 cattgaactc cggattgtca aacagcagcg gcgaatagtc gctgcgatct ccaaaatact
     3661 cccgcgtggt tttcacccag tcgtcggatt catcgttttt cttttcccgt cgcgggatga
     3721 tgaacacttt gccatgcagg gttctcacgg gaacatgcag cacccacccg taaaaaaccg
     3781 tcgtcaccac ccgcgtgcgc ggagtgaaca caagcccgcg tcccatcgtg tcgcttgtct
     3841 gcaccttgag ctcgtagagc cagaagggaa tattccggta tttgccgctg atgcagtcca
     3901 gccgttcaaa atgcgaatag ttctcgcgga aaagacccgc tttcatgaac gaatccggtt
     3961 tgagcccgtg cgaggaataa tattttgcgg tcagcccgtt ttcattcagc aggccgggta
     4021 gaatggattt tttgaaaacg cgggaaagct ccgttggttc aggaagaatg acatggatga
     4081 atgcgcccag cccgaagatc acgacaaata tccagatgat gctgaagaca agcagccagg
     4141 aataatcggt tacaaaggcc agcaccagcg ggatcccggc gaggaacggc gcaaggaacc
     4201 acagcgagaa acgcttctcg gaaaatgtcc ttttccggtc gagctttttc agcgtgctga
     4261 tcgtctgtgc gggcaggttt acagcaacgg acggttcggg ggtttcaagg ttcacggcgg
     4321 agaaaacagg aggaagaaat gaaaaaagat cggtttgcag gtcggtatcc gggacaacgt
     4381 tagaaaaaaa gcgcaggaat tccttctttt tttgattgtt tttgtcaaag gaaaacgacc
     4441 gaaaaatagt taacaaatag ccgcggtttt ccaataaaag atttcaacat ttccctttag
     4501 ttttcagagt gtagcaaaga gtattaacaa catttcaaca cggccggaat tcctcgcgca
     4561 gaaggataaa tttgtatgct tatgacggaa caactcagtc tcattccccc cgcactgcgc
     4621 acctcgcgcc ccgtgcattt tccgaagatc ctgagagacc gctacaacct gctcgtacca
     4681 aggcaggaag acgggctcat cgtgctcgcg ctgtaccaga aggttcgcgc cggcattgtc
     4741 gaacgggcat tccccgagcg catcatcgtg gaaacgatca aaacggtctt cgaagagctc
     4801 ggtcacggct cctcgcgcgg ggaggaaagc cagaagtacg acgaaacgct gcatcgcctg
     4861 ctgcgtcact tcctcgacaa gaacatcgaa gagaaaacct accagctcag ctcgtacgcg
     4921 gaaaatttct gcgacctgct cgagcgtgaa gtgcataccg cgatcaatcc ttcgcagatc
     4981 cagaaaacgt tcaacgacct ggtgatgctg ctcggcctca agctcgagaa catggaggat
     5041 ttccgccact ggtaccacac acagttcttc aagcacaaga acgacatcag cgcgcagctc
     5101 cgcgcgctcc aggcgcagat cgaagacacg gtcgaccagc tcaacgagct cgtgaagaac
     5161 gaggttgaca atttccgcga aatgctcatc ggctgcgaag cgc
//
LOCUS       DASTSF010000185         4241 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_717259,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000185 DASTSF010000000
VERSION     DASTSF010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4241
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_717259"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            357..875
                     /locus_tag="VFU15_05395"
     CDS             357..875
                     /locus_tag="VFU15_05395"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015264968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEU4717242.1"
                     /translation="MNIDKDKVVSVNYHLTVPGDNEGTEVTIEKTSTEEPFVFLFGAG
                     QLLPQFETNLSGKTVGDKFDFRIDAEEGYGNYSIEHVVNLPIENFLDESGKLDSEMIA
                     VGKNVPMMDSDGHRLWGKILEVALNHVRMDFNHPLAGKQLHFEGEVLDVRPATAEELE
                     HGHVHGPGGHHH"
     gene            complement(1120..1392)
                     /locus_tag="VFU15_05400"
     CDS             complement(1120..1392)
                     /locus_tag="VFU15_05400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717243.1"
                     /translation="MTLREDLLKKYFSKSDIGGTESINDLRDRDFNGDALSDEERRAL
                     HNFDRYRIEELNGIKSDMEFHERYRQLQVMANLADWKDFLKLKFPE"
     gene            complement(1470..2396)
                     /locus_tag="VFU15_05405"
     CDS             complement(1470..2396)
                     /locus_tag="VFU15_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014781721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HEU4717244.1"
                     /translation="MKKTSLCIHGGAGVITRGSLSPELEKEYRRGLEDAMTCGLKILS
                     SGGNAIDAVEAAVVSLEDNPLFNAGKGSVLTHEGVCEMDAALMDGSTGKAGSVAGVTT
                     IKNPVRLARLVMERSAHVMLAGKGAEEFARTNGAEEMPADYFLTELRKQQWEKMKNSD
                     SVSLDHDGNKKFGTVGAVALDTNGNLAAATSTGGMTNKRWGRIGDSPVIGAGTFADHV
                     VAISCTGHGEFFIRHVVAHEISALMRMRNMTLADAAGLLVDTVLKNAGGEGGLIAVDH
                     DGNIALPFNTPGMYRAWTDASGNIRTGVYRES"
     gene            complement(2401..2715)
                     /locus_tag="VFU15_05410"
     CDS             complement(2401..2715)
                     /locus_tag="VFU15_05410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717245.1"
                     /translation="MTDLDFPGARWTGLCVLLICALSATGITGLILSLIQKKGLEGNA
                     GAVMKASVISCALPVSGALLSFMTKRLLHVLDLCALPLVFAVILCFSIFYARFSPGDM
                     NK"
     gene            complement(2724..>4241)
                     /locus_tag="VFU15_05415"
     CDS             complement(2724..>4241)
                     /locus_tag="VFU15_05415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717246.1"
                     /translation="WGMVNFFLAFLSKEYAVVLIPLIPAGLMIFHKRKFSDLLYLALP
                     IAGVVLLYVICRIGSVGLASQPVDKRSQDPLNDPYLYATSEQRIASKLNRLDDYIKLL
                     IYPFPLVSDYSYQHFPYSRLSDPDVLLSMLLCVLILPALTIWLWIKRSPYAFAMLIYL
                     GFFALVCNIFFDIGATMGERLIYHSSLGFCMIVAMLLVRLGEVLKESGRYVVTGIFLL
                     ISIPAWMLTTKRNLEWKNDFTLFTSDVKKHPNSALCNGNAGARYMDLGLKYVGHDSIT
                     PNGVVRVGRNERLLHAYADTAMTYLLKAVELHKKYVNGYLNLGLCYYYKDDYENAASA
                     WGNAYLYFPSNPILLSYEQMLIGKANERASKKDFAGAERFFKCAVTTMPNDYSAWSNL
                     AGSSFMARDFATSANAFQQAINLKPDMKNQLINGLRAATSDRMRLDAWKKDSSSYETN
                     LNLGLGYTGTTEFYPESRRLLNKALVAKPGDARVTFLLDSLSGLEQKAAQSPKSK"
BASE COUNT          966 a         1108 c         1194 g          973 t
ORIGIN      
        1 gggcataagg gaagtcggga aagtggagaa aggtcttttt ctgttgctgg cgggagaagg
       61 ctaaggtaga aatttatcgc tgcggtttaa atgtaaagtt ttcgtttaaa atccggattt
      121 caggcggcag ggtgaaaaga agaagtaaac tttatgtgta ttcgcgtcct gtattccata
      181 agaacaaaga tccgcgcctg ttcggaccgg gtacgcgcat ttcaccgctg cgtccatcca
      241 tggtttcaaa ccggttgtac gatggcatcc gtacgcgcct gcgtatagcc atccgtaatg
      301 cacacgcact tctttttgcc gtaccttgcg aaaaaattaa ccaattctgc atcacaatga
      361 atattgataa agacaaagtc gtatcggtaa attaccacct gaccgtgccc ggggacaatg
      421 aaggaacgga agtgaccatt gaaaaaacgt caacggaaga gccctttgtg ttccttttcg
      481 gcgcaggaca attgctgccg cagtttgaaa caaacctgtc aggaaaaacc gttggcgaca
      541 agttcgattt tcgcatcgat gcggaagaag gttacgggaa ttattccatc gaacatgtcg
      601 tgaatcttcc cattgagaat tttctggatg aaagcggcaa gctcgattcg gagatgatcg
      661 ccgtaggcaa gaacgtcccg atgatggaca gcgacggcca ccgcctctgg ggaaagatcc
      721 tcgaagtggc gctcaaccac gtacgcatgg atttcaatca tccgctcgcc gggaaacaac
      781 tgcatttcga aggcgaagtg ctcgacgttc gtcccgccac cgccgaagag ctggagcacg
      841 gacacgtgca tggcccgggc ggacaccacc actgagaatg atgaattata aatgctgaat
      901 tctgaatgaa accggtcgcc gaccggtttt ttttatcccg ggtgagcacg gaaaagaagc
      961 gttcgtttac cggtcgatgt cattttgaac gtgatgtgta atctttccac gcagaacatg
     1021 tttgaccggc ggggaatacg ttccaaacct ccgggaaaag gatcagcgta aaacgaaaaa
     1081 agcccgtcac tgacgggctt cgtaatctct tctgaaaaat cattccggga atttcagctt
     1141 caggaaatcc ttccagtcgg cgaggttggc catcacctgc agctggcggt aacgttcgtg
     1201 gaattccata tctgatttta tgccattcag ttcctcgatg cggtaacggt caaaattatg
     1261 cagcgcacgg cgttcttcat cggaaagcgc gtccccgttg aaatcccggt cacggagatc
     1321 attgatgctt tccgttcctc cgatgtcgga cttggaaaaa tattttttga gtaaatcctc
     1381 gcgaagtgtc ataggtggtg tttgcaaatg taacgttttg gccctgcgcg gggttacagg
     1441 gtttcgccgc ttttttcaac agtgctttgt caggattcac ggtaaacgcc ggtacggata
     1501 ttcccggaag cgtctgtcca cgcgcggtac atgcccggcg tgttgaacgg aagcgcgatg
     1561 ttcccgtcat ggtcaaccgc aatgagcccg ccttcgccgc ccgcattttt gagcacggtg
     1621 tcgacgagca gtccggctgc atccgcgagc gtcatgttgc gcatgcgcat gagcgcggaa
     1681 atttcgtgtg cgaccacgtg gcggatgaaa aattcaccgt ggcccgtgca gctgatcgcg
     1741 acgacgtgat cggcaaacgt tccggccccg atcactggtg aatcgccgat gcggccccag
     1801 cgtttgttcg tcatcccgcc ggtggaagtg gcggcggcga gattcccgtt tgtgtccagc
     1861 gcaacggcgc ccacggttcc gaatttttta tttccatcgt gatcgagcga aacggaatcg
     1921 ctgtttttca ttttctccca ttgctgtttc cgcagctccg tgagaaaata atccgccggc
     1981 atttcctccg ctccgttggt gcgtgcaaat tcttccgcac cttttccggc gagcatcacg
     2041 tgcgccgatc tttccatcac cagtcgcgcg agcctgaccg ggttctttat cgtcgtgacg
     2101 ccggcaacgc ttcccgcttt tcccgtgctt ccgtccatga gcgccgcgtc catttcgcaa
     2161 acgccttcat gcgtaagcac ggaacctttt cctgcattga acagcggatt gtcttccagg
     2221 gacacgacgg ccgcttccac cgcatcgatc gcattcccgc cggacgaaag gatcttcagt
     2281 ccgcacgtca tggcgtcttc gagtccgcgc cggtattctt tttcaagctc cggggaaagc
     2341 gatccgcgcg tgatcacgcc cgcgcccccg tgaatgcaga gactggtttt tttcatgtga
     2401 ttatttgttc atgtcgccgg gtgagaaccg ggcgtaaaaa atgctgaagc agaggatgac
     2461 ggcgaacacg agcggcaacg cgcagagatc gagcacgtgc agcagccgtt tggtcatgaa
     2521 ggaaagcagc gcaccggaaa ccggcagcgc gcatgaaatg acggaagctt tcatcacggc
     2581 acccgcattt ccttcgagtc cttttttctg aatgagcgaa aggatcaacc cggtgattcc
     2641 tgtcgcggaa agcgcgcaga taagcagaac gcacagtccg gtccagcgtg ctcccggaaa
     2701 atcaaggtca gtcatccgcc ggcttacttc gatttcggcg actgggccgc tttctgctcg
     2761 agtccggaaa gactgtcgag caggaatgtg acgcgtgcat cgcccggttt tgcaacaagc
     2821 gctttgttga gcagccgccg cgattcagga tagaattccg tcgtgccggt gtagccgaga
     2881 ccgaggttga ggttcgtttc gtaggaagac gaatcttttt tccacgcatc gagccgcatc
     2941 ctgtcggatg tcgcggcgcg gagtccgttg atcagctggt tcttcatgtc gggcttgaga
     3001 ttgatcgcct gctggaaggc gttcgcagag gtggcaaagt cgcgcgccat gaagctggaa
     3061 ccggcgagat tcgaccacgc gctgtaatca ttcggcatcg tggtgacggc gcatttgaag
     3121 aagcgttccg caccggcgaa atcttttttc gaggcgcgct cgttcgcttt cccgatcagc
     3181 atctgctcat agctgagcag gatgggattc gacgggaaat agagatacgc atttccccac
     3241 gcgctggccg cattttcata atcgtccttg taataatagc agaggccgag gttgagatag
     3301 ccgttcacgt atttcttgtg cagctccacc gctttcagga ggtaggtcat cgcggtgtct
     3361 gcatacgcgt gaagcaggcg ctcattcctt ccgacgcgga cgacgccgtt cggcgtaatg
     3421 gaatcatgcc cgacgtattt gagaccgaga tccatgtagc gtgcgccggc gttgccgttg
     3481 cagagcgcgc tgttcgggtg tttttttacg tcggaggtga agagcgtgaa atcgttcttc
     3541 cattcgaggt tgcgttttgt cgtgagcatc caggcgggta tgctgatcag caggaaaatg
     3601 ccggtgacca cgtatcgccc gctttccttc agcacttccc cgagtctcac cagcagcatc
     3661 gcaacgatca tgcagaagcc gagcgaagaa tgatagatga gacgttcgcc catcgttgcg
     3721 ccgatgtcga agaaaatatt gcacaccagc gcgaagaagc cgaggtagat gagcattgcg
     3781 aacgcgtaag ggctgcgttt gatccagagc cagatcgtaa gagcgggaag gatgagaacg
     3841 cagagcagca tcgacagcag gacatccgga tcgctcaggc gcgaatacgg gaaatgctgg
     3901 taggaataat ccgacacgag cgggaacggg tagatcagca gcttgatgta atcatccagc
     3961 ctgttcagct tggaagcgat gcgctgctcg ctggtagcgt agaggtacgg atcattcagc
     4021 ggatcctgcg aacgtttatc cacgggctgg ctggcaagtc caacggaacc gatcctgcag
     4081 atcacgtaaa gcagcacgac gcccgcaatg ggaagcgcca ggtaaagcag gtcggagaat
     4141 ttccgcttgt ggaagatcat cagtccggcc gggatgagcg ggataagcac gacggcgtat
     4201 tctttcgaaa ggaaagcgag aaagaaattc accattcccc a
//
LOCUS       DASTSF010000186         2091 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_927746,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000186 DASTSF010000000
VERSION     DASTSF010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2091
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_927746"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..385
                     /locus_tag="VFU15_05420"
     CDS             <1..385
                     /locus_tag="VFU15_05420"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HEU4717247.1"
                     /translation="HDYADTGVYHVQQIVVNQWGCEDTLDYDVVIVPYTTLFIPNTFT
                     PNGDGKNDVFMPVGQFVADFHMMIFDRWGNLIYETNDQSMGWDGHANGGNEKAQIDTY
                     VYVVTFHEMYTGNYHRIIGNVNLIR"
     gene            535..>2091
                     /locus_tag="VFU15_05425"
     CDS             535..>2091
                     /locus_tag="VFU15_05425"
                     /inference="COORDINATES: protein motif:HMM:NF024962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprB repeat-containing protein"
                     /protein_id="HEU4717248.1"
                     /translation="MSKALRHFILFALFLQPLFVSGQQNNSSTGCPGVPGACGYHPGN
                     PNARSSNPNPSPQNGNNTLGVIYDQAKCGLDYAAASQRLGQRFSPVGVAQPAPFAISG
                     IPACAVIEKAYLWAEGSGNGAAQTATVNGPLGSANYPMTIVGQGGDKCWGYAGSYTYR
                     ADVTPSVSGNGTYNISGILTNPPTSGNDMDGATLVVIWSNPGANWQGRVVIADGAIIV
                     NGGTSNYNLPISPAVCGATTNARAFCGIGDIQMPVGSLTLNGTPDPIVWNWWNFEQVN
                     TTVASGATTANFNCNTGGDCFNFCIAGLYFRTTTCATCPTTTAFNVTPTSTPATCSAC
                     NGTASVTVSPAGAYTYSWSPAPGSGGNTANPTNMCAGTYTVSVSTSCATVTATVAVTT
                     AGGGVTTTGTQSNVTCNGACDGSASVTVTSGTAPYTYNWTPAPGGGQGSANATGLCAG
                     TYSCDVTDNTGCTGTQTFNITQPTAITITPLQVNVTCAGSCNGSASVNAAGGSGSYTY
                     NWTPAPGAGQG"
BASE COUNT          477 a          594 c          560 g          460 t
ORIGIN      
        1 gcacgattac gcggatacgg gagtttacca tgtgcagcag atcgtcgtta accagtgggg
       61 atgcgaggat acgctcgact acgacgtggt catcgttcct tacacgactt tgttcattcc
      121 gaatacgttc acgccgaacg gggacgggaa gaacgatgtg ttcatgcccg taggacaatt
      181 cgtcgctgat tttcacatga tgatcttcga tcgctgggga aatctcattt acgaaacgaa
      241 cgaccagtcg atgggatggg acggtcacgc caatggcggg aatgaaaaag cccagatcga
      301 cacgtacgtt tacgtggtga cgttccatga aatgtatacg gggaattatc accgtatcat
      361 cggcaacgtc aaccttatcc ggtaacggtt tcccattatt tcgtcgggca aatacctctt
      421 ttcattgctc ttcttgcctc gttggcttat ttttttggaa atcagtgact tttatgctat
      481 tttgcttcgg caaaccatga actgattttc aaccgaattc caaaacctgg caatatgagt
      541 aaagctttac gccatttcat attgtttgcg ctgtttctcc agccgctttt tgtgagcggc
      601 cagcaaaaca attcttccac aggatgcccc ggagttcccg gagcatgtgg ttatcatccc
      661 ggaaatccga atgcgcgatc ttccaatccc aacccgtctc cgcagaatgg taacaacaca
      721 ctgggtgtga tctatgacca ggcgaaatgc ggacttgatt atgctgcagc ttcgcagcga
      781 ctcggtcagc gattttctcc tgtgggtgtg gcacagcccg caccgttcgc catctcggga
      841 attcccgcct gcgcggttat cgaaaaagca tatttgtggg cagaaggttc cggcaacggc
      901 gcggcgcaga cagcaacggt caacggtccg ctgggctccg ccaattaccc gatgacgatc
      961 gtcggccagg gcggcgataa atgctggggc tacgccggtt cgtacactta ccgggcagac
     1021 gtgacaccat ccgtcagcgg aaacggaacg tacaacatca gcggtattct gacgaatcct
     1081 cccacatccg gaaacgacat ggacggagcg acgcttgtcg tgatctggag caatcccggg
     1141 gccaactggc agggtcgtgt cgttattgcc gatggagcga tcatcgtcaa cggcggaaca
     1201 tcgaactaca accttccgat ctctcccgcc gtttgcggcg ctacgacaaa tgcgagagct
     1261 ttctgcggga tcggcgacat ccagatgccg gtcggttctc ttacactcaa tggaacaccc
     1321 gatcctatcg tgtggaactg gtggaatttc gaacaggtca atacaaccgt agcgtccggg
     1381 gccaccactg cgaacttcaa ctgtaatacc ggcggcgact gtttcaactt ctgcatagcc
     1441 gggttgtatt tccgcacaac tacctgcgct acctgcccga cgacaaccgc attcaacgtt
     1501 acgccaacat ccacaccggc gacctgctct gcctgcaatg gcacggcttc cgtcacggtt
     1561 tcaccggcgg gagcgtacac ctacagctgg tcgcctgctc cgggttccgg aggtaatacc
     1621 gcaaacccga caaacatgtg tgcgggtaca tacaccgtca gcgtaagcac atcatgcgca
     1681 acagtcacag cgacggttgc tgttactacg gcgggaggag gcgtcacgac gacaggaacg
     1741 caaagcaacg ttacctgcaa cggcgcctgt gacggatccg cttcggtcac ggtaacttcc
     1801 ggtactgctc catatacgta caactggacc cccgcaccgg gcgggggaca gggcagtgcg
     1861 aacgccacgg gcttatgcgc gggtacgtat tcgtgcgatg taacggataa taccggttgt
     1921 accggcacac agacattcaa catcacgcag cccacagcga tcacgatcac gccactccag
     1981 gtgaacgtga cgtgcgcggg ttcctgcaac ggttctgctt ccgttaacgc ggcgggcgga
     2041 tcaggttcgt atacgtacaa ctggacaccg gcgcccggtg caggccaggg t
//
LOCUS       DASTSF010000187         8231 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1384716,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000187 DASTSF010000000
VERSION     DASTSF010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 8231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8231
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1384716"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2079)
                     /locus_tag="VFU15_05430"
     CDS             complement(<1..2079)
                     /locus_tag="VFU15_05430"
                     /inference="COORDINATES: protein motif:HMM:NF024962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprB repeat-containing protein"
                     /protein_id="HEU4717249.1"
                     /translation="MTKTFTSLFLLAALVFSPSIIPAQSSSCPGVPGACGYPKNNTSA
                     QRPSLSPMDGSGTLGNVYTVSKCGLNFTAGSVKLGQRFTPAGAPQPAAISITGLPSCD
                     SIEKAFLWVEGSGTGASQTVTLAPPSGPAQNFPMTVIGTGPDKCWGYTGSYTYYADVT
                     SAITGNGNYMISNVLTNPPNAGEDMDGATLLIVYCDPMGAYQGTIVIDDGAIEISGGV
                     ANYDMTYPAVCGATQNAQAFCCVGDIQMPVASLTLNGTNAPFTWDWWNYVSVGTTVSI
                     GQTTSNFNLNTPNDCFNFCVAGLYYQTTTCATCQPSPTTLALTPTSTQASCSACDGTA
                     SVTPNPSGPYNYSWLPGGQTSQTATNLCSGSYTVTVSANCILSTATVTVSNPANLAAT
                     AAQTNVSCNAGSDGTATVSPSGGTGPYTYSWSPSGGTGPTASGLSAGLYVCTITDANG
                     CTFQQGVIITQPAPMALNVIGTNVQCNGMNNGSAVAAVSGGSPGYSYSWSPAGGTGDT
                     ATALSPGNYSVTITDANGCSAGGSVAITEPPVLTDMITTVPVGCFGMGSATASPSGGS
                     GSYTFQWQTGGTSATEANLTVGTYTVLITDANGCFLVDTANITSVGAITVQPSYTNIS
                     CFGNNNGTATVNSSGGNPPYSYSWSPSGGNNATATNLTPGTYTCTITDANGCTATQTF
                     NIVQPPQITST"
     gene            complement(2293..5469)
                     /locus_tag="VFU15_05435"
     CDS             complement(2293..5469)
                     /locus_tag="VFU15_05435"
                     /inference="COORDINATES: protein motif:HMM:NF038133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor L domain-containing protein"
                     /protein_id="HEU4717250.1"
                     /translation="MNQKKYVSSGHSHRPGLVLLFLLAGLMINLPMKAQLTVNTSVTA
                     NQLISSLLGGGLNVSNVTLNCAPGAYGTFSNGNTTNIGINNGIMLTTGQAANAIGPNN
                     SASAGTCNGTSGADPQLTALEPLATEDPCILEFDMVPTCNNLQISFVFGSEEYPEFVN
                     SGYNDAFGFFITGPGPACQAGYYNNTNVATLPNNTTIVTIDNVNNVNNSAYYVDNTSG
                     TTIQYDGFTTILTRNVTLCPCQTYHFKLAIADAGDCYYDSGVFVDFMQCSTALAATSS
                     FTPVSGCSACDGTATATASGGNPPYNYSWSPTGGTSATATGLCPGTYTCTITDQGGCT
                     QPQTVTVTIPASGGVTATSTQTNVTCSGGNNGTATVTPANGSGNYSYSWAPSGGTSAT
                     ATGLGPGTYTCTITDITTGCNTTQSFTITQPAPLSTSISGTDVTCNGGTNGSATTTPS
                     GGSPGYTYSWAPSGGTGATANGLSAGSYTVTVSDANGCTTTQSVALTEPTALTVSVTS
                     TAASCSMNTGTATASPSGGTPGYTYSWAPSGGTSATASNLAGGSYTVTVTDANGCTTT
                     QPIAVAQTTALASTTAATDVACFGGTTGSATVTPTAGNPPYTYSWSSGGTSAVETGLG
                     AGTYTVDISDNGGCTGTETITITQPTQLNATISSTDVACNGGTTGSATTSPSGGTPGY
                     TYSWAPSGGSNASASGLSAGSYTVTITDANGCTTTQNATVTQPPALTSTSSSTNVLCN
                     GASTGSASVNASGGTGTYTYSWTPSGGTGASAANLAAGSYTCNITDANGCTASQSFTI
                     TQPSALSVSTSPSAVCAGITTTVSATPSGGTGPYSYSWSNGSTSSSFTVSPVASTSYS
                     VTITDANNCTTTGVDAVTVNSSPTASFSTNAVNGVFSLNGPTGDLCFTDNSSANTVLW
                     NWQLGSDTSTAQNPCVTITPQDIGSYCAQLTVATANGCWDTAEVCIEITNITYSIPNV
                     FTPNGDGTNDFFLITNTGMSSLHCEIYDRWGALIYEWNDPAGYWDGRTNSGKDAVDGV
                     YYYVVYMTDVTGKNYNETGFVHLIRGSK"
     gene            complement(5518..>8231)
                     /locus_tag="VFU15_05440"
     CDS             complement(5518..>8231)
                     /locus_tag="VFU15_05440"
                     /inference="COORDINATES: protein motif:HMM:NF024974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HEU4717251.1"
                     /translation="NGYCQWPINAAIPSNADPMNSIMAPWHDIDPSVGTPTSATDVNW
                     QVFGTAPCREFCISWNDVAMYDCNNLIATSQVILHETTNIIDMYIQNKPLCTSWNGGA
                     AIEGIQDASGTNAYSVPGRNYPTQWTASNDGWRFMPSGTPNYSIVWAGPSGTIGTTNT
                     VNVCPTATTSYTATVTNASCSGPIVVSDVVTVNVTNSMSASVTSTPASCTSNNGTATA
                     TPSGGSGNYTYNWAPSGGTSATATGLSAGTYTVTISDANTGCQTTQTVNVTNTGNLSS
                     SNTTTSISCNGGTASSTVTPVGGTGPFTYSWAPTGGNSSTATGLAAGTYTCTVTDANG
                     CTSTQVINVTQPPALTASNNITNVDCNGNSSGAVTVTPSGGSGGYMYTWAPAGGSNPT
                     ASNLPSGTYSCTITDSNGCTTTSTANVTEPAALSVSVSVAPVSCTGTTTATANVSGGT
                     GTYNYAWSSGGNAQTEGNLSPGSYTLTVTDANNCAVTSQFNVVPPVTMTVTSTFTDAS
                     CFGGSDATATVNPSGGTAPYTYSWSPSGGTNATATGLAPGTYTCTITDAGGCTATQTV
                     SVSQPTQLSSTTTSNPATCFAYSDGSGSVTAAGGTPGYTYSWAPSGGTAQSATGLQAG
                     TYTVTTTDANGCITTQSVTVTQPTQLNVTTTPDAVCAGSQATISASASGGTAPYAYSW
                     QGGGTSATNTVTPVSTTVYTVSITDANNCTTTGSVTVTVNPGPVAAFGTNAINGVFSL
                     PGPTGNLCFTDNSSSNVVAWYWQMGTDTSTSQNPCVTVTQQNAGTYCAELTVTTAAGC
                     TDTADACIEINNVSYFIPNVFTPNGDGTNDVFKITNTGMSSLHCEVYDRWGNLVYEWN
                     DPTGFWDGKVKSGNEAVDGVYYYVVDMADFTGKNYNETGFVHLIKGN"
BASE COUNT         1609 a         2091 c         2554 g         1977 t
ORIGIN      
        1 cgttgatgtt atctgcggag gctgcacaat gttgaatgtt tgtgtggccg tacatccgtt
       61 cgcgtcggtg atcgtgcagg tataagtgcc gggagtcagg ttcgtcgccg tggcattgtt
      121 tccgcccgac ggagaccagc tgtagctata aggcgggttt ccaccggatg aattgacggt
      181 tgccgttccg ttgttgttgc cgaaacagga aatgttcgtg tacgaaggtt gcacggtgat
      241 cgcaccgaca gaggtaatgt tggccgtgtc gacgaggaaa caaccattcg catcggtgat
      301 cagcacggtg taggtgccaa cggtgaggtt cgcttccgtg gccgaagttc caccggtctg
      361 ccattggaaa gtgtaagatc ctgaaccgcc ggaaggactt gcggtggcgc ttcccattcc
      421 gaagcagccc accggcactg tggtgatcat gtcggtgagc acgggaggct cggtgatcgc
      481 aacggaacct ccggccgagc agccgttggc atctgttatc gttacggaat aatttccagg
      541 gcttagtgcg gttgcggtat cgcccgttcc gccggccgga ctccatgagt aagaatagcc
      601 gggcgagcct ccggaaaccg cagcgaccgc ggaaccgtta ttcattccgt tgcactgaac
      661 atttgttccg atgacgttga gcgccatcgg tgcaggttgt gttataataa caccttgctg
      721 gaaggtgcag ccattggcat ccgtgatcgt gcagacatac aggccggcgc tgagcccgct
      781 tgctgtcgga cctgttccgc ccgaaggaga ccagctgtat gtgtaaggtc ccgttccccc
      841 gctcggcgaa acagtggcgg tgccatcaga cccggcgttg caggaaacgt tggtttgcgc
      901 agctgtcgcg gcaaggttgg ccgggttgga aacggtgacg gtcgccgtgg aaagaatgca
      961 gttggcgctt accgtaacag tgtaagagcc cgaacagaga ttggttgccg tctgtgaagt
     1021 ttgtcccccg ggcagccagg aataattgta gggcccggaa ggattgggcg tcaccgatgc
     1081 ggttccgtcg catgcggagc agctggcctg cgtcgatgta ggcgtgagcg caagcgtggt
     1141 cggactgggc tggcacgtgg cgcaggtggt tgtctggtaa tacaacccgg ccacgcagaa
     1201 gttgaagcag tcgttgggtg tgttgaggtt gaagttcgat gttgtctgcc cgatgctcac
     1261 ggtcgttcct acggaaacat aattccacca gtcccacgtg aacggcgcgt tcgttccgtt
     1321 gagcgtgagc gaagcgaccg gcatctgtat gtccccgacg cagcagaatg cctgcgcgtt
     1381 ctgtgtggct ccgcagacgg cgggatacgt catgtcgtaa ttggcaacac cacctgaaat
     1441 ttctatggcg ccgtcgtcga taacgatcgt tccctggtat gcgcccatgg gatcgcagta
     1501 aacaatgaga agtgtcgcgc cgtccatgtc ctcgccggcg ttcggtgggt tggtgagcac
     1561 gttgctgatc atgtaatttc cgttccccgt gatcgccgag gtgacgtccg cgtaataagt
     1621 gtatgatccc gtgtatcccc agcatttgtc cggcccggtt ccgatgaccg tcatcgggaa
     1681 attctgcgcg ggcccggaag gtggggcgag cgttactgtc tgtgaggcgc cggttcccga
     1741 accttccacc cagagaaaag ctttttcgat cgaatcgcag gacggaagcc cggtaatgga
     1801 aatggcggcg ggctgcggcg cacctgcggg tgtgaatcgc tgtccgagct tgaccgagcc
     1861 ggccgtaaag ttcagcccgc atttggaaac ggtatacacg ttacccagcg ttccgcttcc
     1921 gtccatcggg gaaagggaag gtcgctgcgc ggaagtattg tttttcgggt acccgcatgc
     1981 gcccgggact ccgggacagg aagagctttg tgcagggata atcgatggag aaaagacgag
     2041 cgctgcgagg aggaaaagac tggtaaaggt tttggtcata caccgatgtg ttgcaaataa
     2101 gtttttaaaa tacaaagacg cccgggattg gccaaatcgc ggaggtaaaa gatagtggcc
     2161 agccctggtt tccccgctaa actgcgggcc tttgctcttt gcggtccggt ctttattccg
     2221 caaaagcgtc cggggcacga attttcccgt ttccggtgaa caaaaaaaaa ccggccggag
     2281 ccggttcaga tcttatttgc tgcctcgtat caggtgaacg aatcccgttt cattgtaatt
     2341 cttgccggta acgtccgtca tgtaaacgac gtaataatac actccgtcca cagcatcttt
     2401 cccgctgttc gttcttccgt cccagtaacc tgcaggatcg ttccattcgt aaatgagcgc
     2461 accccagcgg tcgtagatct cgcagtgcag cgatgacatt cccgtgttgg tgatcaggaa
     2521 aaagtcgttt gttccgtcgc cgttgggtgt aaatacgttc gggatcgaat acgtgatgtt
     2581 ggtgatctcg atgcatacct cggcggtgtc ccagcatccg ttcgcggtgg caacggtaag
     2641 ctgcgcgcaa taagagccga tgtcctgcgg tgtaatggtc acgcacggat tctgcgcagt
     2701 ggaagtatcg gagccgagct gccagttcca caggacggta ttggcagatg aattgtctgt
     2761 gaagcagaga tctcctgtag gtccattgag cgagaatacg ccgttgacgg cattcgtact
     2821 gaaagaagcg gtcgggcttg aattcacggt aacagcatca acgccggtcg tcgtacagtt
     2881 attggcatcg gtgatagtta cggaatagga tgttgatgca accggtgaaa ccgtgaagct
     2941 ggaagatgtt gaaccgttcg accagctgta cgaataagga cccgttccgc ccgaaggtgt
     3001 tgcggaaaca gtggtggtga tccctgcaca tacggcagaa ggtgacgtcg acacgctgag
     3061 cgcggaaggt tgtgtgatcg tgaacgactg tgatgcggtg cagccattgg cgtcggtaat
     3121 gttgcatgta taagaacctg ccgcaagatt ggccgcagat gcacccgttc cgccggaagg
     3181 tgtccagctg taagtgtaag tgcctgttcc cccggaagcg tttacagatg ccgaccccgt
     3241 tgaagcgccg ttacaaagca cgttcgtgga agaagacgtt gaggtaagcg ccggcggttg
     3301 tgtaacggtt gcgttctgcg tggtcgtgca tccgttcgca tcggttatgg tcactgtgta
     3361 gcttcccgca gaaagtcccg atgccgaagc gttgcttcct cctgacggcg cccacgaata
     3421 ggtgtagccg ggtgttccgc cggaaggact cgttgtggca gaaccggtag ttcctccgtt
     3481 gcatgccaca tcagtggatg agatcgtcgc attgagctgc gttggttgag tgatcgtaat
     3541 ggtttccgtt cccgtacagc ccccgttatc gctgatgtca acggtgtagg ttcccgcacc
     3601 gagcccggtt tcaactgcgg atgttccgcc gcttgaccag gaataggtgt aaggcggatt
     3661 gcctgcggta ggcgttacgg ttgcgcttcc ggttgttcct ccgaagcagg caacgtccgt
     3721 cgctgcggta gtggaagcga gcgcagtggt ttgcgcgaca gcgataggtt gtgttgttgt
     3781 acaaccattc gcatcggtga cggtgacagt atagctgccg ccggcaagat tggatgctgt
     3841 tgcggaagtt cctccggacg gcgcccatga gtaagtgtaa ccgggtgttc ccccggacgg
     3901 agaggctgtt gcggttccgg tattcatgct gcagcttgcc gcagtcgaag taacggatac
     3961 tgtcaatgct gttggttcgg tgagcgccac gctctgcgtt gtcgtgcagc cgttggcgtc
     4021 ggaaactgtt acagtgtagc ttcccgcgga gagcccgttc gcggtggcgc ctgttccgcc
     4081 cgaaggcgcc caggaatacg tgtagcccgg tgatccgccc gacggtgttg ttgtcgcgct
     4141 gccgttggtt cccccgttgc aggtgacatc tgttcccgat atcgatgtgc tcagcggagc
     4201 cggttgcgtg atcgtgaacg attgtgttgt attacaacct gtggttatat ccgtgatcgt
     4261 gcatgtatac gttcccggtc ccaggcctgt cgcggttgcg gatgtcccgc cgctcggcgc
     4321 ccagctgtaa gagtagttcc ccgatccgtt ggccggagta acggttgccg ttccgttgtt
     4381 gccgccgctg caggttacgt tcgtctgcgt ggaagtcgcc gtcacgccac cgcttgcagg
     4441 aatagttacc gttactgttt gcggctgcgt gcatcctccc tggtcggtga tggtgcaggt
     4501 gtaagtgccg ggacagagtc ctgttgcagt tgcgcttgtt ccgcctgtgg gcgaccatga
     4561 gtaattgtaa ggaggatttc cgccggaagc cgtggctgtc gcagttccgt cgcaggccga
     4621 gcatcctgag accggtgtga aagaggaggt cgcggcgagt gctgtggagc actgcatgaa
     4681 atcgacgaac acacctgaat cgtaataaca gtcgcctgcg tctgcaatgg cgagcttgaa
     4741 gtgatacgtc tggcacgggc acagcgttac gttccgtgta agtatagtgg taaacccgtc
     4801 atactgtatc gtggttccgg aggtattgtc tacataatac gccgaattgt tcacattgtt
     4861 cacgttgtcg atggtaacga tggtggtgtt attaggcaac gtcgccacgt tcgtgttgtt
     4921 ataataacct gcctggcacg cagggccggg accggtgatg aagaatccga aggcatcgtt
     4981 gtacccgctg ttgacgaatt cgggatattc ttctgagccg aagacaaagc ttatctggag
     5041 attgttgcag gtcggtacca tatcgaattc caggatgcac ggatcctcag tagcgagcgg
     5101 ctcgagcgcg gtgagctgtg gatccgctcc cgatgttccg ttgcaggtac ctgcgcttgc
     5161 agagttgttc gggccgatcg cattggcggc ctgcccggtg gtcagcataa tgccgttgtt
     5221 gatgccgatg ttggtcgtgt tgccgtttga gaatgttccg taagcgccgg gagcgcagtt
     5281 cagcgtcacg ttgctgacgt tcagtccccc gccgagaagc gaagagatga gctggttggc
     5341 ggtaaccgaa gtgttgaccg tcagctgcgc tttcattggc aggttgatca tcagtcctgc
     5401 cagcaggaaa aggagaacaa ggccggggcg atgactgtgc ccggaggaaa catatttttt
     5461 ctgattcatg ctattggtgt ttcactttgc atactgcagg cgcaaagctg tgaaggatta
     5521 attgcctttt atgaggtgaa cgaaaccggt ttcgttgtaa ttctttcccg tgaaatcagc
     5581 catgtcgacc acataatagt acacgccgtc gacagcctcg ttcccgcttt ttacttttcc
     5641 atcccagaag ccggtgggat cgttccattc atacacgaga tttccccagc ggtcgtaaac
     5701 ttcgcaatgc agggaggaca tcccggtatt cgtgatcttg aatacgtcgt tggtcccgtc
     5761 gccattgggt gtgaaaacat tcggaatgaa ataggaaacg ttattgatct cgatgcacgc
     5821 atcggcagta tcggtgcagc ccgccgcggt ggtaacggtc agctctgcgc aataggtgcc
     5881 ggcgttctgc tgtgtaacgg tcacgcatgg attctgtgaa gtggaagtat cggttcccat
     5941 ttgccagtac cacgctacca cgttgctgct ggaattatcc gtgaagcaga gattgcctgt
     6001 aggtccggga agcgagaaaa cgccgttgat cgcgttcgtg ccgaatgccg caacggggcc
     6061 gggattcact gtaacagtaa cactgccggt ggtcgtgcag ttattggcgt ctgtgatcga
     6121 aacggtataa acggttgtcg atacgggagt gacggtattt gttgcgctgg ttcctccgcc
     6181 ctgccagctg tatgcataag gcgcggttcc acccgaagca gaagccgaga tcgttgcctg
     6241 cgagccggcg cacacagcgt caggtgtcgt ggtgacgttc agttgcgtag gttgggtcac
     6301 ggtaacggat tgtgttgtga tgcaaccatt cgcatccgtt gttgtgacgg tataagttcc
     6361 ggcctggaga ccagttgccg attgtgccgt tccgccgctg ggcgcccagc tgtaggtata
     6421 gccgggagtt ccaccggcag cagtaacgct tccgcttcca tcgctgtagg caaagcatgt
     6481 ggccgggttg cttgttgttg ttgaagaaag ctgcgtgggc tgtgaaacag aaacagtctg
     6541 cgtggcggta catccgccgg cgtcggtgat tgtgcaagtg tacgtaccgg gtgcaagacc
     6601 tgttgccgtt gcatttgttc cgccggaagg agaccacgaa taagtatacg gcgccgtgcc
     6661 cccggaagga ttcacggtgg cggttgcgtc actgccgccg aaacagcttg catccgtaaa
     6721 cgtggaagtg acggtcattg tcaccggcgg caccacattg aactggctgg tgactgcgca
     6781 gttattcgca tcggtaacag taagcgtata gcttcccggt gagaggtttc cttcggtctg
     6841 cgcgtttccg ccgctcgacc aggcgtaatt ataggttcct gttccgccgg aaacgtttgc
     6901 agtcgcggta gtggttccgg tgcagctcac aggggcaaca ctcacagaaa cgctgagcgc
     6961 agccggttcc gttacgtttg cggttgatgt cgttgtacat ccgtttgagt cggtgatcgt
     7021 gcagctgtat gttccggaag gaaggttcga tgcggtcggg ttcgacccgc ctgcaggagc
     7081 ccacgtatac atataacctc cgcttccgcc tgaaggtgta accgtgaccg ctccgctcga
     7141 gttgccgttg caatccacgt tcgtgatgtt attcgaagcg gtaagcgcag ggggctgtgt
     7201 gacgtttatg acctgcgtgg acgtgcatcc gttcgcatcc gtaaccgtgc aggtatatgt
     7261 gcccgctgca agaccggtcg ccgttgatga gtttcctccc gttggcgccc agctgtaagt
     7321 gaaaggtcct gttccgccta cgggtgtcac cgtcgaggat gcggttcctc cgttgcagga
     7381 aatagacgtt gtcgtattgc tggaagaaag attgccggtg ttggtcacgt ttacggtctg
     7441 cgtggtctgg caaccggtgt tcgcatcgct tatggtaacc gtataagtgc ctgcggaaag
     7501 tcctgtcgct gttgcggaag ttccgccgga aggcgcccag ttgtatgtgt aattgccgga
     7561 acccccggaa ggagtcgccg tcgctgttcc gttattcgat gtgcaggacg caggagtgga
     7621 ggtaacgctc gccgacatgc tgttggtcac attgacagtg acgacatcac tgaccacgat
     7681 cggtcccgag cagcttgcgt tcgtgactgt ggccgtatag cttgttgtgg cagtaggaca
     7741 gacattcacc gtattggtcg tgccgatcgt accgctcgga cctgcccaga cgatagaata
     7801 attcggcgtc cccgaaggca tgaatcgcca gccgtcattc gaggctgtcc attgcgtcgg
     7861 gtaattccgc ccgggaacgg aataggcgtt tgttccggaa gcatcctgta tcccttcaat
     7921 agccgcaccg ccgttccagg atgtacacaa cggtttgttc tggatgtaca tgtcgataat
     7981 gttcgtcgtc tcgtgcagga tcacctgcga agtggcgatg aggttattgc agtcgtacat
     8041 cgccacgtca ttccagctga tgcagaattc gcggcagggt gctgttccga aaacctgcca
     8101 gttcacgtcg gtggcggaag tcggtgttcc gactgaagga tcgatatcgt gccatggcgc
     8161 catgatggag ttcataggat ctgcattcga agggatcgca gcattgatcg gccactggca
     8221 atagccgttc g
//
LOCUS       DASTSF010000188         3003 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_737800,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000188 DASTSF010000000
VERSION     DASTSF010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3003
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_737800"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..280)
                     /locus_tag="VFU15_05445"
     CDS             complement(<1..280)
                     /locus_tag="VFU15_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019296697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEU4717252.1"
                     /translation="MPLPGNTPLLRLEKLVAPGCADVYVKYEGANPTGSMKDRMALSM
                     IEGAERRGQLKPGGTVVEYTGGSTGSSLAMVCATKGYKARFVSSDAFSG"
     gene            complement(618..956)
                     /locus_tag="VFU15_05450"
     CDS             complement(618..956)
                     /locus_tag="VFU15_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007810102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHCW motif cupin fold protein"
                     /protein_id="HEU4717253.1"
                     /translation="MSHIPFQTIDWSAIEKTEHKGESGTAFWRTIRFGGLRVRMVEYS
                     GGYLADHWCEKGHIVHCLEGEFTSELSTGEKIRLVKGETYVVSDGRSSHRSTSENGVK
                     LLVIDGDFLK"
     gene            complement(1077..>3003)
                     /gene="katG"
                     /locus_tag="VFU15_05455"
     CDS             complement(1077..>3003)
                     /gene="katG"
                     /locus_tag="VFU15_05455"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014937541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HEU4717254.1"
                     /translation="YGGLMIRMAWHSAGTYRIADGRGGSNTGNQRFAPLNSWPDNANL
                     DKARRLLWPVKKKYGNKISWADLIILAGNMAYESMGFKTFGFAGGREDIWHPEMDVNW
                     GTEKEWLAATKNRYANETDRGTLENPLAAVQMGLIYVNPQGVDGKPDPLKTAQDVRTT
                     FKRMAMNDEETVALTAGGHTVGKAHGNGNAEKLGESPEGADIKEQGLGWMNPQGKGNA
                     EDTITSGLEGSWTTNPTKWDNGYFHLLFNYEWELKKSPAGAWQWEPVNIKEEDMPLDA
                     HIPGKRRNPIMTDADMAMIKDPEYRKISERFYKDPEYFAKVFARAWFKLTHRDLGPKS
                     RYLGADAPKENLIWQDYVPPVDYTLSGAEIEELKAKLLASGLTRAELINTAWDSARTF
                     RGSDFRGGANGARIRLAPQKDWEGNEPVRLQKVLKTLTEIQSGLKKKISLADLIVLGG
                     SAAVEKAAQEAGVAVNVPFSAGRGDATDEMTDAESFAVLEPIHDGYRNWLKKDYAVKP
                     EELLLDRTQLMGLTAPEMTVLIGGMRVLGTNHGGTKHGVFTDREGVLSNDFFVNLTDM
                     NYSWKPVADNLYNVVDRKTGATRWTATRVDLVFGSNSILRAYAEVYAQDDNKEKFVRD
                     FVAAWVKVMNADRFDLK"
BASE COUNT          569 a          866 c          752 g          816 t
ORIGIN      
        1 ctccggaaaa tgcatccgaa gaaacgaaac gggctttgta acctttcgtc gcgcacacca
       61 tcgccagcga gctgcccgta cttcctcctg tgtattcaac taccgttcca cccggtttca
      121 gctgccctct tcgctccgca ccttcgatca tcgacagggc catgcggtct ttcatgctgc
      181 ccgtcgggtt cgctccttcg tatttcacgt agacgtcggc gcagccggga gcgacaagtt
      241 tttcgagcct gaggagaggt gtgtttccgg gaagcggcat cgggttcagg agtttcggat
      301 cagtaagaat agctaaaatt aagaatactt acacgggcag gaattgtaat tttcctgaag
      361 caatctgctg ctttgtccgg gtttggcttt tcaagcggag gcgactaaaa tcgccggcgt
      421 aaacaggcgt tgcattcaca ttcagcgatc acgcatgcgg gaagaaaaaa atattgcgcc
      481 ggaagaagat tgctaattcc tgttttgaaa tctttccggc cgggaaagct tattcatgca
      541 ggcgctggag ccgcgtttcg ctcctccggt gaaaacggag ttctttgcgc gttcgatacc
      601 tgcgatgata ttctttctta tttcaggaaa tccccgtcga tgaccaggag cttcacaccg
      661 ttctccgatg tggaacgatg cgaactgcgg ccgtcggaaa cgacgtacgt ttctcctttg
      721 acaaggcgaa ttttttcacc ggtgcttagt tcgctggtaa attctccctc gaggcaatga
      781 acaatgtgtc ctttttcgca ccagtgatcg gcgagatacc cgccggaata ttccaccatc
      841 cgcacccgga gtccgccgaa ccggatcgtg cgccagaacg cggttccgct ttctcccttg
      901 tgctccgttt tttctatggc tgaccagtca atggtttgaa aagggatgtg gctcattttg
      961 ttcggatggt tttattgaca gtgcctcgac tcgcgcctga tgcggcagtg gaatttattt
     1021 gtgcctcgac tggcgctcgg cacgacttgc gcccggcacg acatccgact cgcgaattat
     1081 ttcagatcga aacgatcagc attcatcact ttcacccagg cggctacaaa atccctgacg
     1141 aacttttctt tgttgtcgtc ctgcgcatac acttctgcat acgcacgcag gatggaattc
     1201 gaaccaaaga caaggtctac ccgcgtagca gtccacctgg tagctcctgt tttccggtcg
     1261 acaacattgt acaggttatc ggcaaccggt ttccaggaat agttcatgtc cgtaaggttc
     1321 acaaagaaat cattgctgag cacgccttcc ctgtccgtaa acacgccgtg cttcgttcca
     1381 ccgtggttgg tgcccagtac gcgcatgcca ccgatcagaa ctgtcatttc cggtgctgtc
     1441 agtcccatca gctgtgttct gtcgagcagc agctcttccg gttttacggc gtaatctttt
     1501 ttcagccagt tcctgtaacc gtcgtgtatg ggttcgagca cagcaaacga ttccgcatcc
     1561 gtcatttcat cggttgcatc tccgcggccc gcgctgaacg gaacgttgac ggccacgcct
     1621 gcttcctgcg ccgctttttc caccgcagcg cttccgccca acacgatcag gtcggcgaga
     1681 ctgatttttt tcttcagccc cgattggatt tccgtaagag ttttcaatac tttctgcagg
     1741 cgtacgggct cgttgccttc ccagtctttc tgcggagcaa gacggatacg tgcgccgttc
     1801 gctccgcccc tgaaatcaga acccctgaac gttcgtgcgc tgtcccacgc cgtgttgatg
     1861 agctctgcgc gggtaagtcc gctggcgagg agcttcgctt tcagttcttc aatttcagcg
     1921 cctgacaatg tgtaatcgac cggaggcaca taatcctgcc agatcaggtt ttccttcggc
     1981 gcatctgcgc caaggtagcg gctcttcgga ccgagatcgc ggtgcgtcaa tttgaaccag
     2041 gcacgcgcaa aaactttcgc aaagtattcg gggtctttgt aaaaacgttc tgaaatttta
     2101 cggtattcgg gatctttgat catcgccata tcggcgtccg tcatgatcgg gttccggcgc
     2161 ttgccgggaa tgtgcgcgtc cagcggcatg tcttcttctt tgatgtttac aggttcccac
     2221 tgccatgcgc ctgccggact ctttttaagt tcccattcgt aattgaacag caaatgaaaa
     2281 taaccgttgt cccatttcgt cgggttggtt gtccatgaac cttcgaggcc gctcgtaatc
     2341 gtgtcttccg catttccttt tccctgcggg ttcatccatc cgaggccctg ctcttttata
     2401 tccgcgcctt cgggactttc gcccagcttc tctgcatttc catttccgtg cgccttgccg
     2461 actgtatgtc cgcctgcagt gagcgccact gtttcttcgt cgttcatcgc catgcgcttg
     2521 aacgtggtgc gtacgtcctg cgccgttttc agcgggtcag gttttccgtc cacgccctgc
     2581 gggtttacgt agatcagtcc catctgcaca gccgccagcg gattttccag cgtaccgcgg
     2641 tccgtttcat ttgcgtaacg gtttttggtt gcagccagcc attctttttc cgttccccag
     2701 ttgacgtcca tttcaggatg ccagatgtct tcacgtcctc ccgcaaaacc gaatgttttg
     2761 aagcccatcg attcgtaagc catgttcccg gcgaggatga tcaggtcggc ccatgaaatt
     2821 ttgttgccgt attttttctt gaccggccat aacaacctgc gcgccttgtc gagattggca
     2881 ttgtcaggcc agctgttcag cggagcgaaa cgctggttgc ccgtgttgct tccgccgcgc
     2941 ccgtcggcaa tgcggtacgt tcccgccgaa tgccacgcca tgcggatcat cagtccgccg
     3001 tag
//
LOCUS       DASTSF010000189         3806 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_208496,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000189 DASTSF010000000
VERSION     DASTSF010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3806
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208496"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            167..1246
                     /gene="rlmN"
                     /locus_tag="VFU15_05460"
     CDS             167..1246
                     /gene="rlmN"
                     /locus_tag="VFU15_05460"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017731226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEU4717255.1"
                     /translation="MNTEGKNIRTLTLEELKTFFTEKGEKAFRAKQVWEWLWQKSARG
                     FDEMTNLSKGTRELLSQSFSFPVVRIDNFQVSRDRTIKNAFRLHDGNIVEGVLIPTES
                     RMTACISSQVGCSLTCKFCATGKLARLRNLDAEEIYDQVVLIRDQAMKNYNSPLTNIV
                     YMGMGEPLLNYRNTLDSVERITSPDGLGMSPQRITVSTAGIAKMIRKLGDDGVKFNLA
                     LSLHAANDKKRDYIMPINEQNTLEALAESLNYFYDKTGTRVTFEFIVFKDFNDSVEDA
                     EELAAYCRKVPAKVNIIEYNPIDDGEFRQTLPSRLDAFKNALEEKGVIVNVRRSRGKD
                     IDAACGQLANKNDAVKKEEIAFKKK"
     gene            complement(1335..2078)
                     /locus_tag="VFU15_05465"
     CDS             complement(1335..2078)
                     /locus_tag="VFU15_05465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717256.1"
                     /translation="MRIFLLTALLFLASFPQAAGQTVLHTAGDSSDVKSKVKVRVINP
                     HGKKPIDYTGVAIHTDSVQMAIKINPLNALRGDFPAYFEWRVSDPFSVEVALGATYID
                     YMYELVNNGGQYILDANERRNVKFYSGFTGRFQLRWYPSNSETAITGFYIAPEFARRT
                     YRMDYYVNTGLIREPHPIHRNYTDFRLQIGWQNADPYENVFLEWFASAGIRFQQTDWI
                     EGSGTEAVFKSDSWASFVVGGGLKIGFTL"
     gene            2127..3671
                     /locus_tag="VFU15_05470"
     CDS             2127..3671
                     /locus_tag="VFU15_05470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717257.1"
                     /translation="MRKKILLLLLFTAVLAPLFAQMEITWGVPQKEPHKTYTSEVLGE
                     SDGYLYSVRSVYSIWGPRNPFIERYRMSDMSLDYSKELALDAGDKARSEEGIFLCGNT
                     VVVFASYYDKDRSMNVLQGEVLDDHCVAKTQWKEIARIDASKKANPGSFYLGLGQDSV
                     HFLIVINPPYDKYANEKFRLDYLRTDLSPEWEKEIVPPYQDQYFSLTNFIASETGDVY
                     MLATISKDKSVMTKKERRTQPTYYHTVLMYSAASDSLREYKVSIDPKFISDIRMTVND
                     SGDIICSGFYSNKSSNAIVGTFYLRIDRKTSQVSSIGTKDFDQDFLTQFMSAKKASEG
                     KELYDYDLKHLVLRDDGGAVLVAEQYYEVLVQTYDPATKMYSYTYYYYYNDIIVISID
                     SSGAISWAKKIPKYQVSRNDGGYYSSFAFAVSGDRMYFMFNDSPRNTGQEDSRHIHAM
                     NNPRKSVAVLVTMDSKGNIDRQAMFSNRDLKIVLRPKLFMQFNEHRMLLYGEKGSTFK
                     FADVRM"
BASE COUNT          981 a         1019 c         1023 g          783 t
ORIGIN      
        1 aggtcctacc tataatatga ccggcacgca atgcccctga agcgcggcag caaagtcctt
       61 gaaaaccatc ctcgcgggtg gtttttctat ttctggcaat ggctatttcc tttcagtcgc
      121 ggaaacctga aggaagacgg gcatttcctg tcgtaccttt gcagcaatga acaccgaagg
      181 aaagaacatc cgcacgctca cactggaaga gctgaagacc tttttcacgg agaaaggcga
      241 aaaagcgttc cgtgcaaagc aggtatggga atggctgtgg cagaaatcgg cgcgcggttt
      301 tgatgagatg acgaatcttt ccaaaggaac acgtgagctg ctgtcgcaaa gcttttcatt
      361 ccctgtcgtc cgcatcgaca atttccaggt gagccgcgac cgcacgatca agaatgcgtt
      421 ccgcctgcac gacgggaaca tcgtggaggg cgtgcttatt cccacggaat cgcgcatgac
      481 cgcgtgcatc tcttcgcagg tcggctgcag cctcacgtgc aaattctgcg caacggggaa
      541 gctcgcacgc ctgcgcaacc tcgacgcgga agaaatttac gaccaggtgg tgctcatccg
      601 cgaccaggcg atgaagaatt acaattcccc gctcacgaac atcgtgtaca tgggcatggg
      661 cgagccgctg ctcaattacc ggaacacgct cgattcggtg gaacgcatca cgtcgccgga
      721 cgggctgggc atgtcgccgc agcgcatcac ggtttccacc gcgggtatcg cgaaaatgat
      781 ccgaaagctc ggcgatgatg gagtgaagtt caacctcgcg ctttcgctcc atgccgcgaa
      841 cgataaaaaa cgcgattaca tcatgcccat caacgagcag aatacgctcg aagcgctggc
      901 ggaatcgctg aattattttt acgacaaaac gggaacacgc gtcacgttcg agttcatcgt
      961 attcaaagac ttcaacgatt ccgtggaaga tgcggaagaa ctcgccgcct actgcaggaa
     1021 agttcccgcg aaagtgaaca tcatcgaata caacccgatc gacgacgggg aattccggca
     1081 aacgctgcca tcgcgtctcg acgcattcaa aaacgcgctg gaagaaaaag gagtgatcgt
     1141 caacgtgcgg cgatcccgcg gaaaagacat cgatgccgcc tgcggacaac tggcgaacaa
     1201 gaatgacgcc gtgaaaaaag aggagatcgc gttcaagaaa aaataaaaag acggccctgt
     1261 ttcacgcaaa gacgcgaaga cgaaaagttt ttttgcgaag aagaatttgc ctgccaaaag
     1321 tcgtgatgat tacgctatag cgtaaaaccg atcttgagtc cgccgccgac cacgaacgac
     1381 gcccagctgt cgcttttgaa aaccgcttcc gtcccgctcc cttcgatcca gtcggtttgc
     1441 tgaaaacgta tccccgcgga agcgaaccat tcgagaaaga cattttcata cggatcggcg
     1501 ttctgccacc cgatctgcag gcggaaatcg gtgtaattcc tgtgaatggg atgcggttcg
     1561 cggatgaggc cggtgttcac gtaataatcc atccggtacg tgcgccgcgc aaattccggg
     1621 gcaatgtaaa aaccggtgat cgcggtttcc gaattcgagg gataccagcg gagctggaaa
     1681 cgcccggtga agccggaata gaatttcacg ttccgccgct cgttcgcatc gagaatgtat
     1741 tgcccgccgt tattgacgag ctcgtacatg taatcgatat acgttgcacc gagcgccact
     1801 tccacgctga acggatcgga cactctccat tcgaagtacg caggaaaatc gccgcgtaat
     1861 gcattcagcg gattgatctt gatcgccatc tgcacgctgt ctgtgtgaat ggctactccc
     1921 gtataatcga tgggcttttt cccgtgcggg ttgatgacgc ggaccttcac cttgcttttc
     1981 acgtcggatg aatcgcctgc ggtatgaagc acggtctgtc ctgcagcctg cggaaacgag
     2041 gccaggaaaa gaagagccgt gagcaggaat attcgcatgc aggaaataaa gtagctttgt
     2101 taaagttaca aaatccacag tcgattatgc gcaaaaagat cctgctgctg ctgcttttca
     2161 cagccgttct cgccccgctt tttgcgcaga tggagatcac ctggggcgtt ccccagaaag
     2221 agccgcacaa aacgtataca tccgaggtgc tgggcgaaag cgatggatac ctctattctg
     2281 tccgcagcgt gtattccatc tggggcccgc gcaacccgtt catcgagcgt taccggatga
     2341 gcgacatgtc gctcgattac tcgaaagagc tggcgctgga tgcaggcgac aaagcgcgca
     2401 gcgaggaagg catcttcctg tgcgggaata ccgttgtcgt gttcgccagc tattacgaca
     2461 aggacagaag catgaatgtg ctccagggag aagtgctcga cgatcactgc gttgcaaaga
     2521 cccagtggaa ggagatcgcg cgcatcgacg catcgaaaaa ggcgaacccg ggcagctttt
     2581 atctcgggct gggacaagat tcggttcatt tcctcatcgt gatcaatccg ccgtacgaca
     2641 agtacgcgaa tgaaaaattc cggctcgatt acctccgcac cgatctttct cccgaatggg
     2701 aaaaggagat cgttccgcct taccaggacc agtatttttc gctgacgaac ttcatcgctt
     2761 ccgaaacagg agatgtgtac atgctggcga ccatttcgaa ggacaagagc gtgatgacga
     2821 aaaaagaaag aagaacgcag cccacctatt atcacaccgt gctgatgtac agcgcagctt
     2881 cggattcgtt gcgggaatac aaagtgagca tcgatccgaa attcatctcc gacatcagga
     2941 tgacggtgaa cgacagcggg gatatcatct gcagcggttt ctattcgaac aaaagcagca
     3001 acgcgatcgt gggaacattt tacctgcgca tcgaccggaa gacgagccag gtttccagca
     3061 taggcacgaa agatttcgac caggatttcc tgacgcagtt catgagcgcg aagaaagcgt
     3121 cggaaggcaa agagctgtac gattacgacc tgaagcacct cgtgctgcgc gacgacggag
     3181 gcgccgttct cgtggccgaa cagtattacg aagtgctggt gcagacgtac gacccggcca
     3241 cgaagatgta ttcctacacg tactattatt attacaacga catcatcgtg atcagcattg
     3301 attcctcggg agcgatctcg tgggcgaaga agatcccgaa ataccaggtg agccgcaacg
     3361 acggcgggta ttattcttcc ttcgccttcg ccgtgagcgg cgacaggatg tatttcatgt
     3421 tcaacgacag ccccaggaac accgggcagg aagacagccg ccacattcat gccatgaaca
     3481 atccgcggaa atcggtggcc gtgcttgtga ccatggacag caaagggaac atcgaccggc
     3541 aggcgatgtt cagcaaccgc gacctgaaga tcgttcttcg gcccaagctg ttcatgcaat
     3601 tcaacgagca cagaatgctg ctgtacggcg aaaaaggcag cacgttcaag ttcgccgacg
     3661 tccgcatgta gccttcccgc ggagggattt ttcccactac ctttgaattc cctttatgtc
     3721 ccgcgtcgac gaaatcaagc agccggtagc tgccgaaatg cagatcttcg aagagaagtt
     3781 ccgcgattcc atgaagagct ccgctc
//
LOCUS       DASTSF010000190         2425 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_700776,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000190 DASTSF010000000
VERSION     DASTSF010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2425
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_700776"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..726
                     /gene="tyrS"
                     /locus_tag="VFU15_05475"
     CDS             <1..726
                     /gene="tyrS"
                     /locus_tag="VFU15_05475"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEU4717258.1"
                     /translation="QMGGSDQWGNIVTGTELIRRRDGGEAWALTTPLIKKADGTKFGK
                     TEGGNVWLDPKRTSPYDFYQFWINAADADAGNYIRIFTLKGKEEIETIEAEHNEDPGK
                     RVLQKLLAEDITKRVHGTDALNAVIDATAMFMAKNAPELIAAANEETFEKMIAAAGIR
                     RVDLSAEELASGVRLVEILTTRSAMFDSNGDAMKMMKANGIAVNKVKATDPKMTLSAS
                     QLVGGKYSLVQKGRNDFCVVRVL"
     gene            complement(777..2090)
                     /locus_tag="VFU15_05480"
     CDS             complement(777..2090)
                     /locus_tag="VFU15_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU4717259.1"
                     /translation="MKKYFFLPALLFTGSLFAQQPVPAKKQTKSILLMNGTAHIGNGQ
                     VIENSVVGFKDGKITLVGDAKTVRLNMADWDTTINCVGKQIYPGIIAPNSTLGLTDIE
                     AVRATNDFYEVGGYNPHVRSQVAFNTDSKIPPTVRTNGVLLAQVCPRGGRISGQSSIM
                     SLDGWNWTDATLKADDGVHVNWPDTYGRSWSEDDGPGQYQKNKNYEQQKQDLEKFFAE
                     AKAYNDNPSHAEQNQRFEAMRGVFDGTKNLYIHADLVKDITDAITFVKKFAIAHPVIV
                     GGVDSWQVTDLLRQNNIPVMLFRVHSLPTRNEDDVDLPYKTAAMLQNAGVLFCLQNAG
                     DMEAMNSRNIPFLAGTTVAYGMDKEAAIASITGNAAKILGIDKQVGTLETGKDATLFI
                     SDGDALDMKTNNVTWAFIQGRTLDLRNEQQQLYHEYEDKYGLKNK"
     gene            complement(2103..>2425)
                     /locus_tag="VFU15_05485"
     CDS             complement(2103..>2425)
                     /locus_tag="VFU15_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020528558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU4717260.1"
                     /translation="PATLLHLDQHMGSIKVGKDADVVLWSDNPTSVYARVEKTIVDGK
                     LLYDAQDDIKLRDEMHRDRARIIQEMIAEKNGGAPVRLPKVHHLHLWTCDDLITDGSY
                     LSEQ"
BASE COUNT          503 a          633 c          707 g          582 t
ORIGIN      
        1 cagatgggcg gctcggacca gtggggaaat atcgtgacgg gaacggaact tatccgccgc
       61 agggacgggg gagaagcgtg ggcgctcacg acgccgctga tcaaaaaagc ggacgggacg
      121 aaattcggga aaacagaagg cgggaacgtg tggctcgatc cgaaacgcac ttcgccgtat
      181 gatttttacc agttctggat caatgctgcc gacgcagacg cgggaaacta catccgcatt
      241 tttacgctga aaggaaaaga agagatcgaa acgatcgaag cggagcacaa tgaagatccg
      301 ggcaaacgcg tactgcagaa attgctggcg gaagacatca cgaaacgcgt gcacgggacg
      361 gatgcgctca atgctgtcat cgatgcaacg gcgatgttca tggcgaagaa cgcgcccgag
      421 ctgatcgccg ctgcgaatga agaaactttt gaaaagatga tcgccgctgc gggaatccgg
      481 cgggtggatc tttctgcgga agagcttgcg tccggcgtgc gcctcgtgga aatactcacc
      541 acccgttccg cgatgttcga ttcaaacggc gatgcgatga agatgatgaa agcgaacggc
      601 atcgcggtga acaaggtaaa agccaccgat ccgaaaatga cgttgagcgc ttcacagctg
      661 gtcggcggca aatattcgct cgtgcagaaa gggaggaatg atttctgtgt cgtaagggtt
      721 ttgtgaaacg atgaataatg aatgacggat caggattcat tcatcctttc cgttcatcat
      781 ttattcttca gcccgtactt atcctcgtat tcatggtaaa gctgttgctg ctcgttccgc
      841 agatcgagcg tgcggccttg tatgaatgcc cacgttacgt tgttcgtctt catgtcgagc
      901 gcatctccgt ccgaaataaa aagcgtggcg tcctttcctg tttcgagcgt ccccacctgt
      961 ttgtcgatgc cgaggatttt cgctgcgttg cccgtgatcg aggcgatcgc cgcttcttta
     1021 tccatgccat aggcgaccgt cgttccggca aggaagggaa tgttgcgtga attcatcgct
     1081 tccatgtcgc cggcattctg caggcagaaa agcacgcctg cgttctgcag catcgccgcg
     1141 gttttgtagg gaagatcgac gtcgtcctcg ttgcgagtgg gaagactgtg cacgcggaac
     1201 agcatcacgg gaatgttgtt ctgcctgagc aggtccgtca cctgccagct gtccacaccg
     1261 cccacgatga cagggtgcgc gatcgcgaat tttttcacga acgtgatcgc gtcggtgatg
     1321 tctttcacga ggtcggcatg gatgtaaagg tttttcgtgc cgtcgaaaac gccgcgcatc
     1381 gcttcgaaac gctggttctg ttctgcgtgc gaaggattgt cgttgtaggc ttttgcctcg
     1441 gcgaagaatt tttcgaggtc ctgtttctgc tgctcgtaat ttttgttttt ctggtattgc
     1501 cccgggccgt cgtcttccga ccagctccgg ccgtacgtat cgggccagtt cacgtgcacg
     1561 ccgtcgtccg ccttgagcgt cgcgtccgtc cagttccatc cgtccagcga catgatgctc
     1621 gactgcccgg aaattcttcc gccgcgcggg cacacctgcg cgagcagcac gccattcgtg
     1681 cgcacggtcg gcgggatctt tgaatccgtg ttgaacgcca cctgcgagcg gacgtgcgga
     1741 ttgtagccgc ccacttcgta aaagtcgttc gtcgcgcgca ccgcttcgat gtcggtaaga
     1801 ccaagcgtcg agttcggtgc gataatgccg ggatagatct gcttgcctac gcaattgatc
     1861 gtggtatccc agtccgccat gttgagccgc acggtctttg catcgccgac gagcgtgatc
     1921 tttccgtcct tgaaaccgac gacggaattt tcgatcacct gcccgttacc gatatgagcg
     1981 gtgccgttca tcaggaggat gcttttcgtt tgttttttcg ccgggaccgg ttgctgcgca
     2041 aaaagactgc cggtgaaaag aagagcgggt aggaagaaat attttttcat gatcgtgatt
     2101 gcttattgtt cgctgagata gcttccgtcc gtgatcaggt cgtcgcacgt ccacaggtga
     2161 agatggtgca ctttcggcag gcgcacgggc gcgccaccat ttttctcggc gatcatttcc
     2221 tggatgatgc gtgcgcggtc gcggtgcatt tcatcgcgca gctttatatc gtcctgcgca
     2281 tcgtaaagca attttccgtc gacaatggtt ttttccacgc gggcgtaaac ggaagtggga
     2341 ttatcgctcc agagaacgac atccgcatct tttccaactt tgatgctgcc catgtgctgg
     2401 tcgaggtgaa gcagcgtcgc gggat
//
LOCUS       DASTSF010000191        14329 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1626612,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000191 DASTSF010000000
VERSION     DASTSF010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 14329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14329
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1626612"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..542)
                     /locus_tag="VFU15_05490"
     CDS             complement(<1..542)
                     /locus_tag="VFU15_05490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEU4717261.1"
                     /translation="MKNTLPLKQLLRFLILPSVLLLFSAGCSHKKKRNENDPNDTIPV
                     RTAKFFTLSDVHFDPFYDTSLVQKLVHADVNDWDAIFRSSHQTGFGVYGKDSNFNLMT
                     GAFSAMHNVNADPDFIVITGDFLSHKFQQTYDTLTGTKMQDALPREAFITKTIEFIAL
                     ELVKEFPHTPVYPVLGNNDD"
     gene            707..964
                     /locus_tag="VFU15_05495"
     CDS             707..964
                     /locus_tag="VFU15_05495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717262.1"
                     /translation="MAEVKRYELAPLEAEYRSLTTGSDRDAASRMRNFFGVLRYDLRM
                     GFEVVMEYREGMDEPMTMRILRSEKDLDRWIEHRFARLQEF"
     gene            complement(970..1866)
                     /locus_tag="VFU15_05500"
     CDS             complement(970..1866)
                     /locus_tag="VFU15_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009195408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HEU4717263.1"
                     /translation="MEFRDYYKILGVDRNATQDEIKKAYRKLAVKFHPDKNPGDKTAE
                     DRFKEITEANEVLSDPEKRKKYDAVGADWKHYQETNQPNDFDWSQFGGSGNFHSFSGE
                     EGGDFSDFFEQLFGQRSQQRARNRRGQDYAAEMEITLDEAFFGTSRILEVHDEKLRIN
                     TKPGAYEGQQLRIKGKGGKPSGNAARGDIYITIHVQPHPAFQRKGDDLYTDLVVDIFT
                     AVLGGEEEVHTPGGVVKIRVPAGCDSGKKLRVRGKGMPVYGKENHHGDLYVTIGIRVP
                     KELSEEQKELFRRLQAGNTVTH"
     gene            2632..2705
                     /locus_tag="VFU15_05505"
     tRNA            2632..2705
                     /locus_tag="VFU15_05505"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2666..2668,aa:Arg,seq:acg)
     gene            2858..6703
                     /locus_tag="VFU15_05510"
     CDS             2858..6703
                     /locus_tag="VFU15_05510"
                     /inference="COORDINATES: protein motif:HMM:NF026253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HEU4717264.1"
                     /translation="MRPPVFLRLFFAVAFLLLPFFSFAQQKKIIWYDGNWDQASRKLA
                     SYYRVISFDSAGHPAGLVSDYYISGHLQFRGKLVYFSADSEAYDSICTWYYESGAVKS
                     RQLFMNGRDNGFDSVFYENGKLKAALNYSADQKQAHSVSWYPDGHINVIGYRDVESGA
                     GVDSMFYESGRLSGIAHYINGVQEGEFRAWSPGGKLKYVFHYHEGRRDGESITYSSSG
                     CVKRREFYRRDTLTGLSYGLAGKNCDTVFVTFYSSKGFKIYERDYDNNHRLTGILTGD
                     SLTGRTESRQFYPDGHVKSATVSVNRLQYNRNVSFYPDGKIMDVTVYENDREISDSSW
                     YENGMLRSVMLQFPGRPEMDVSFYPDGKKMGETIPDSLLLHIAKDNVACAYGLKNKNE
                     KWVVAPQFSMIYALPDGFFKVTQNGKSGIYSVTGKKIIAPVYDDLDPLYNPANVYYSP
                     GRIAYYPYYGYFIAVNHGKKGLVNSSGAVVLPFSYDDIFLDGSNTRIIVSVNNRNSIA
                     DSTGHVGRATFTSGPQFNGSYGYITEYSRENGYRYGIIDSLGNTVLPARFDEVHICGT
                     NPVTFWVKIHDKAGVMDSHGKIVLDTLYSAPSLEADLRDSAVVVSRNGKTGIASLSGE
                     ILVALLYDSVKITSPSGGYYCSKGKYGIISEHFSKVSPPVYESLEGFTTGMVNDDDDV
                     FRGDAFVAKSKGKYGVITVADSILFPFVYDNIWLGNNEIAFTRNDTLSVRYRSRLDAE
                     VALTDVVTFIDGLAVFSNDAYLRTARSDSYKEGVVDANGRILMKPVYSILHAGRDGIV
                     FTGHDGRSGIRSPEGKITMLPDVYSNFEYRDGLIYVTSFSGKAGVTNMNAKLVIDTAF
                     YGICGAGEGPRMFWVKTTPVYDTVQEMYDYHGGWFLLNSSGKKIANTSWNYPAYFYNS
                     VAVISGKEKQGLMDRSGKLLLDTVYDDITRDDIGYFLVRKNGLTGFADARGKIIVAPR
                     WNAASGFFGSLMFVYDQEGNAGIVDTTGKMICGISRSGILQYPDSLGAQVFFYTSEEA
                     GSNAQGVNARIQFRDLSEMVTGYDSILLLQGRHAQDAAMNFIYSLAAMNYTGYGLMYA
                     FNTTPYYGYIYPYPVTEITDWNVVADDNSDDDEYNDVTYYTSVTGASSLTFSVRQAEV
                     SCYSPPRGMEYCNTVSDSFMTFGVRGDSIFLLTPDSLFDKNKNYPVLINAMLLAALKN
                     SDNPGFDCGDTSNLFERTRNDFAVCNSGVLFYIATGQPGYTDDGENDNRVPVLLDWKV
                     LRPVMAKNSILKILAKHPG"
     gene            6731..7978
                     /locus_tag="VFU15_05515"
     CDS             6731..7978
                     /locus_tag="VFU15_05515"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU4717265.1"
                     /translation="MSLFRDYRRIERYVLFAILAEFCVQLVNATFMNLQTLYMKAEGY
                     HDAEIADFISWRFAGVLVFAFPLGMFIRGKKVRPLFRISSAGVPLFALCILWCVHFHH
                     DNLLIVSQWMWGTCFTFIQIPMIPFILRNCSPENRTAAISLSYSTWSLAGISSAVFVA
                     VTNHINPVLFSERNLMAAISVAAFAGVYFVSRVKTEKEPGVEDKPVSFAAQHDWKLIF
                     SALFPALIIATGAGLTIPFINLFFSRVHHMETDVISAWNFCAALLVAIAALTVPKIKK
                     TIGYRVAVPTTQTFAVVALVLMATTELYNHLAIAGVIAVACYIIRQPLMNVAGPMTTE
                     ISMKYVGKKNREITSALAAAIWSGSWFISTRFIFASLRNIGWPYVDIFLITAAFYMCG
                     IILYVLLIRSYNRREKAGLITEE"
     gene            8148..11078
                     /locus_tag="VFU15_05520"
     CDS             8148..11078
                     /locus_tag="VFU15_05520"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEU4717266.1"
                     /translation="MRKSICLLFFFLSSCSLFSQSPAYVRYNSFSGLPCNEVYDVIQD
                     HLGFIWTATDRGIFRFDGYSFEAFTTAQGLTDNTVFRICEDFRGRIWSMPFNGELCCI
                     DNGRVTVYPFNDTLKNHLPGIRIARSLNVLPSGEVQIGYLIHGIVSVSAEGVFRRIST
                     RKNYKAEYYMMDTGGGLLYGMQETAGMKTGTRLQFTSEGMTDEKLNFEKFSGHQQIAG
                     IRRRNGGWCFALMNELVEISPQGTMTEQPLPKQPLCVYEDNENCLWVTMDAGGGVRRY
                     RPGKTACGETVFDTYYPGESVSRIMQDAEGAFWIATLSHGLFYVPSVHTLLWNIPGDE
                     EDKVVCLAEAGKDCTLALWRDKGLFRLSGGNSPLSPVVLAGDNNSFRWVTFDSLRSRL
                     FIAERRFAEACSWPGLQEEKKWGLSIQSFETGAQGTFCCESWQAAFLDSSDEWKQLGP
                     NPDKQRPDVIFEERGGKTWVGRMDGLFYVSHDTLVPFASPDPVFRKRVSGICSLDDGT
                     LVVATQSSGIAWIKNGETRLLSDENGIITDHVLSIMPGKGNSLWLTTIYGLYNVRNNN
                     GVWEKSVFPEVSCFLSNDGRCYFTDKDGDLWMSTGASVIRFDPDHSFATHYLPPVYLR
                     EIIANGKEISSAQNISLPYDHNFLRISFAGLAYRMHGHLRYRYRLSQEDPQWTYTYSN
                     SVELASLGPGNYTFEVQAENERGVWSSAPAFYSFTILPPVWQMWWFRIAAFLVLSAIF
                     FLLIRYRFRQLKRQAELREQAQDFRQQALALQMNPHFVFNALNTIQAYVLKEDKSRAI
                     EMFSSFAQLLRKSLDHARERYITLAGEIEMLGLYFGIESLRFEERLKYSITVDKNIDT
                     SMTLVPAMLVQPLVENAIRHGIMNRKAGGHVDVRFEMEDGKLFCAVEDNGVGRATAAK
                     LRAGDHRSAGSDITADRLRVLCKMSGAQFYFSVTDKTDKNGNATGTLVRFAIPFTGKK
                     QEGI"
     gene            11075..11791
                     /locus_tag="VFU15_05525"
     CDS             11075..11791
                     /locus_tag="VFU15_05525"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEU4717267.1"
                     /translation="MKRLKALVVDDEPNAVEGFVRLLTLYCPDIEPAGTASSVGEAIT
                     QIRKLKPDVVFLDIEMPEENGFNLVDTIRENSCSLVFVTAHSDYAVRAFRTDAVDYLL
                     KPVSPRELKAAAERVTSRRETMKKTLPDEEYRIRVSTTEGISFIPCAEVISIEGSGRY
                     SIIHSADGGTQLVSRNIGDFETELEPFGFFRVHKSWLINCRHVMRLAAADGGTIELSN
                     GKKVLLSRRRRNDFLKKMER"
     gene            11937..>14329
                     /locus_tag="VFU15_05530"
     CDS             11937..>14329
                     /locus_tag="VFU15_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717268.1"
                     /translation="MKKRFLFNIGFAASLLFNLQHASACDFSDDFSSAVPWTQLGSGV
                     HVSSGNCVYNPAVCGSDQRVFRSLSPTTSMLDNTSWTALVDFMPQTTFSSGVAHSIIS
                     FTSNTLNPYRYTSSGSYTPTDFIEILYSSPINTGFSQWKLYASAKDGANAGQVSSAGI
                     SLPSLNTWYYLELQRSGNTVTVSAFSDPARTIPLSGSPVSLTFTGSITNLAYVQSGSV
                     IQGAASRKLNALIDNICVTGLRAGGNCDVWSDYTSSTGWTQINSGVSITGGNVLYNNV
                     ADGGHIQRRVYESLGGDITGANTWKADFDFYPTAVGTGGPGHTLCGITAGTQDPFCDD
                     SYTATNQDAIAACWISNNHTDDPSTFMLDGRAKDGNGAWSTSAGILLSLGTHYYITLE
                     RLDEFNGRISAFLDAGRTIHAPGSPQCFTIPPGVFNLDYVQHGSIPWGGDDRALTATV
                     DNFCLKADYLNPGTVSYNGPLSFCASGTPSVISSETPATGGNWTWNYQWQQSVNCNGV
                     WTDIAGATGTSYTPSSISTSTCYRRAALGPCGVAVYSNVIGILVYQPLSANITSTETG
                     DHCTASYTLTANPSNPTGVTYLWNTGATTQSIVVGNPNSPTTYSVTVTSPCYTATASY
                     VVYPCYHPSGAFPTISANSWFEVNWSGGCTGSCYLQFIDPAYPVGGGPAYGVTQAELL
                     VFDRWGNMLYDQVYVPSPPCDGLWNGQIIWNGIYMGYPVVDGDYDYVLKRTNCSGNDE
                     VRGSVVVLGTARMASHDADTTVATYTPPLSVVYPNPSAGVFNVDLLPSINNVDVTMEV
                     "
BASE COUNT         3179 a         3709 c         3908 g         3533 t
ORIGIN      
        1 aaatcatcgt tgttcccaag cacaggatag accggcgtgt gcggaaattc tttcacgagc
       61 tcgagcgcga tgaactcgat ggttttcgtg atgaacgctt cgcgcggaag cgcatcctgc
      121 atcttcgtgc cggtcagcgt atcgtaggtt tgctggaatt tgtgcgagag gaaatcgccc
      181 gtgatcacga taaaatccgg atcggcattc acgttatgca tggcgctgaa tgcgcccgtc
      241 atgagattga aattggagtc cttcccgtaa acgccgaacc cggtttgatg tgagcttctg
      301 aagatcgcgt cccagtcgtt tacatcggcg tgcacgagct tctgtaccag cgaagtatcg
      361 tagaacggat cgaaatgcac atcggaaagc gtgaagaatt tcgcggtgcg gaccgggatg
      421 gtatcgttag gatcgttttc attccgcttc tttttatggc tgcacccggc tgaaaaaaga
      481 agcagcacgg acggaaggat caaaaaacgg agaagctgtt tcagcggaag ggtgtttttc
      541 atagcgggaa gagtaggttt attatgaaga cggtgaaggt cgtcaatgaa tggagaaata
      601 tccgaaataa acccctcgtt ccgtctttgc ggctcgtgat tatttatcca cgcgcaatca
      661 ccttcccgcg ccggatgccc cgcgatttta cagctacatt tgccgcatgg cagaggtgaa
      721 acgatatgaa ctggccccgc tggaagccga gtaccgcagt cttaccacgg gaagcgatcg
      781 tgacgctgct tcgcgcatgc ggaatttttt cggggtgctg cgctacgacc tgcgcatggg
      841 gttcgaagtg gtgatggaat accgcgaagg aatggacgaa ccgatgacga tgcggatact
      901 gaggtcagaa aaagatctcg accgctggat cgagcaccgg tttgcgcgcc tgcaggaatt
      961 ctgaaatgat cagtgcgtaa cggtgttccc ggcctgcagc cggcggaaca attctttctg
     1021 ctcttccgaa agctctttcg gaacgcgtat gccgatggta acgtaaagat cgccgtggtg
     1081 attttctttt ccgtaaaccg gcattccctt tccgcgcacg cgtaattttt tcccgctgtc
     1141 gcacccggcg ggaaccctga tcttcacaac gcctcccggt gtatgcactt cttcctctcc
     1201 gccgagtaca gccgtaaaaa tatcgacgac gaggtccgtg tacaggtcat cgccttttcg
     1261 ctgaaaagca gggtgcggct gaacgtgtat cgtgatgtaa atgtcaccgc gtgccgcatt
     1321 gccggaaggt tttccacctt ttccttttat gcggagctgc tgtccttcat acgcaccggg
     1381 tttggtgttg atgcgcagct tttcatcgtg tacttcgaga atgcgggatg ttccgaaaaa
     1441 ggcttcatcc agcgtgatct ccatttccgc agcataatcc tgtccgcgcc ggttgcgtgc
     1501 gcgctgctgt gaacgctgtc cgaacaattg ttcgaagaaa tcggaaaaat caccgccttc
     1561 ttctccgctg aacgaatgga aatttccgct gccgccgaat tgcgaccagt cgaaatcgtt
     1621 cggctggttc gtttcctggt aatgcttcca gtcggcaccc acggcatcgt attttttccg
     1681 tttctccgga tcgctcagca cttcgttcgc ttccgtgatc tccttgaaac ggtcttctgc
     1741 tgttttgtcg cccggatttt tatcgggatg aaatttaaca gccagcttcc ggtacgcttt
     1801 tttgatctcg tcctgcgtgg cattgcggtc gacgccgagg attttataat agtccctgaa
     1861 ttccatcctg tatgttttaa aaaaaggagg cttacgggcc tccttcgttt ttccgcgagc
     1921 cttacggggc tccgtttgtt ttgcaaagcc ttacggggct tcgcttgttt ttacagaacc
     1981 ttacggggct ccgtttgatt ttgcaaagcc ttacggggct tcgcttattc tggcagagac
     2041 ttgcaggtcc ctgtttaatt gggaagcctt acggggcttc gcctgatcta ccggcaaaag
     2101 cctcacagct ttcacctttt tcccggttat cttttcacct ttcaccttga cgcgacaacc
     2161 ggttctgtca aatcctttca cttaaattcc tttcaacgtt ccaaaagtaa atcgcggtaa
     2221 acgggtaaaa catgaggccg gtcaaagcat ggtgtgaggg ttgtcagcgc acggtttctg
     2281 cgcgcttccg ttgcctgcgc atggcaagcc tgtttcattg caagcgtgat gagaacgggt
     2341 gtacgctcac aatgaagacc ggtttcgaaa gtgccgttgc gctggcgctc cggtttttcc
     2401 ttcaacgacc tgccgcaagc cgcgggagat ttgaaagagt gatttctccc ccggattgct
     2461 ggttttctca aaatctttat ctttgccgtc cgcttcccgg tcgtagctca atccgtcttc
     2521 gcggagctac gacggacagg ctggatagag cacctgacgg agcttgttcc ggcggatgac
     2581 ggaagatcag aagacctggg gttcgaatca tccccaataa aattttaaaa tggttccgta
     2641 gctcaactgg atagagcatc tgactacgga tcagaaggtt tggggttcga atccctacgg
     2701 gaccacaaaa aatgaagggc ttgcgcaggc aggcccattt tttttaccgg cgattgaccg
     2761 tcaacagtta tccggcattt tccggcaact gaaagaaccc gccccgttcg cttcccaaac
     2821 attgtaattt gcggcaaaat caatcccgga agaagctgtg agaccccccg tttttcttcg
     2881 cctgtttttc gctgttgctt ttttgctgct tccgtttttt tctttcgcgc agcagaaaaa
     2941 gatcatctgg tacgatggaa actgggacca ggcgtcgcgc aaactggcgt cctattaccg
     3001 cgtgatctcg ttcgacagtg cgggtcatcc cgccgggctg gtgagcgatt attacatcag
     3061 cggtcacttg cagttccggg gaaagctggt ttatttttct gccgattcgg aagcgtacga
     3121 cagcatctgc acatggtatt atgagagcgg cgccgtgaag agccggcagc ttttcatgaa
     3181 cggccgggac aacgggtttg attctgtctt ttacgagaac ggaaagctga aagcggcgct
     3241 gaattattcg gctgaccaaa agcaggctca ttccgtttcc tggtatcccg acgggcacat
     3301 caacgtgatc ggttaccgcg atgtggaaag cggtgccggc gtcgattcga tgttttacga
     3361 aagcggacgc ttgtcgggga tcgctcatta tatcaacggg gtgcaggaag gagaattccg
     3421 tgcctggtcg ccgggcggga agctgaaata cgtttttcat tatcacgagg gacggcgcga
     3481 cggagaatcc atcacgtatt cgtcttccgg ctgcgtaaag cgaagggaat tttaccgtcg
     3541 tgatacgctg acgggcctga gctacgggct cgcgggaaaa aactgcgaca cggtgttcgt
     3601 cactttttat tcttcgaaag gcttcaaaat ttacgaacgt gattatgata acaaccatcg
     3661 cctcaccggg atcttaaccg gcgattcgct gacaggaaga acggaaagca ggcagtttta
     3721 cccggacggt cacgtgaagt ctgcgactgt ttccgtgaac cgtttgcagt acaaccggaa
     3781 cgtttcgttt taccccgatg gaaagatcat ggatgtaacc gtgtatgaaa acgatcggga
     3841 aatttccgat tcctcctggt atgagaatgg catgctgcgt tcggtcatgc tgcaattccc
     3901 gggtcggccg gaaatggacg tttcatttta cccggatgga aaaaagatgg gtgaaacgat
     3961 ccccgattcg ctgctgcttc acattgcaaa agataacgtg gcctgcgcgt acggacttaa
     4021 aaacaaaaat gagaagtggg tggtggctcc gcagttcagc atgatctatg ctttgccgga
     4081 tggttttttc aaagtgacgc agaatgggaa atcgggaata tacagcgtca ccgggaaaaa
     4141 gatcatcgca cctgtatacg acgatcttga tccgctgtac aatcccgcga acgtctatta
     4201 ttcaccgggc cgcattgctt actatccgta ttacggttat ttcattgcgg tgaaccacgg
     4261 gaagaaagga ctcgtgaact cctccggcgc tgtggtattg ccgttttcct acgacgatat
     4321 ttttctcgac gggtcgaaca cccggatcat cgtatctgtc aataaccgga actccattgc
     4381 cgattcgacc gggcatgtcg gccgcgcaac gtttacttcc ggtccgcagt tcaacggaag
     4441 ctacgggtac atcactgaat acagcaggga gaacgggtat cgttacggca tcatcgattc
     4501 ccttggcaat acggtacttc ctgcgcgttt tgacgaagta cacatctgcg gaacgaaccc
     4561 ggtgaccttc tgggtgaaga tccatgataa agcgggcgtg atggattcgc acgggaagat
     4621 tgttctcgat acgctttatt ccgctccgtc gcttgaggcg gatcttcgcg acagcgccgt
     4681 ggttgtttcg cgaaacggga aaaccggcat tgcttcgctc agcggggaaa ttcttgtcgc
     4741 gttgctctat gattctgtaa agatcaccag cccgtcgggc ggatattact gcagcaaggg
     4801 gaagtacggc atcatcagcg aacatttttc gaaagtttct cctcctgttt acgaatcgct
     4861 ggaaggattt acgacaggga tggtgaacga cgatgacgac gttttccgcg gcgatgcgtt
     4921 cgtggcaaaa tccaaaggaa agtacggcgt gatcacggtt gccgattcca tcctcttccc
     4981 gtttgtctat gataatatct ggcttgggaa caacgagatc gcattcacgc gcaacgatac
     5041 actttccgtg cgttaccgct cgcggcttga tgctgaagtc gcattgacgg acgtggtcac
     5101 gttcatcgac ggtcttgccg tgttcagcaa tgacgcttac ctgcgcaccg cccgttcgga
     5161 tagctataaa gaaggcgtgg tggatgcgaa cgggagaata ctgatgaagc cggtgtattc
     5221 catcctgcat gccggaagag acggaattgt ttttacaggg catgacgggc gcagcggcat
     5281 ccgttcaccg gaggggaaga tcactatgct gccggatgtg tacagcaatt tcgaataccg
     5341 tgacggtttg atttacgtca cctccttttc cgggaaagcg ggagtgacaa acatgaacgc
     5401 gaagctggtc atcgacacgg cgttttacgg gatatgcggg gccggggaag gtccccggat
     5461 gttttgggtg aaaacgacac cggtgtatga taccgttcag gagatgtacg attatcacgg
     5521 cggctggttc ctgctgaatt cgtcgggaaa aaagattgca aatacctcct ggaattatcc
     5581 cgcgtatttc tataattctg tcgcggtcat ttcaggaaaa gaaaaacagg ggcttatgga
     5641 caggagcgga aagctgctgc tcgatacggt ttacgatgat attacgaggg atgacatcgg
     5701 ttatttcctg gtcaggaaaa acgggctgac gggatttgcg gatgcgcgtg gaaagatcat
     5761 cgttgcgccg cgatggaatg ccgccagcgg ttttttcggt tcgctcatgt tcgtttacga
     5821 ccaggaggga aatgcaggca ttgtcgacac aacgggaaaa atgatctgcg ggatcagccg
     5881 ttcgggtatc ctgcagtatc ccgatagtct cggtgcgcag gtgttctttt atacaagtga
     5941 agaagcaggc agtaatgcac agggcgtaaa tgcccggatc cagttccgtg atctttccga
     6001 gatggtcacg ggttatgatt cgatcctcct gctgcaaggc aggcacgcac aggatgcagc
     6061 gatgaatttc atctacagcc tcgccgcgat gaattatacc ggttacggtc tcatgtacgc
     6121 gttcaacaca acgccttact acgggtacat ttatccttac ccggtcacgg aaattacgga
     6181 ctggaacgtg gtggcggatg ataacagcga cgacgatgaa tacaacgacg tgacgtacta
     6241 cacatccgtc acaggcgcat cttcgcttac attttccgtg cgccaggcgg aggtatcctg
     6301 ttattctccg ccgcgcggca tggagtattg caacaccgtt tccgattcat tcatgacttt
     6361 cggcgtgcgc ggcgattcga tcttcctgct gacgcccgat tcgctgttcg ataaaaacaa
     6421 aaattacccg gtgctcatta acgccatgct tcttgctgcg ctgaagaaca gcgataaccc
     6481 cggttttgac tgtggcgaca cgtcgaacct tttcgaacgg acaagaaacg attttgcggt
     6541 ttgcaacagc ggcgtcctgt tttacatcgc cacgggacag cccggttata cggatgacgg
     6601 agaaaacgat aaccgtgttc ccgtcctgct cgactggaaa gtgctgcggc ccgtgatggc
     6661 gaagaacagc atcctgaaaa tcctcgcgaa gcatcccggc tgatcattcc cttgtatctt
     6721 cgccgcgtct atgtcccttt tccgcgacta ccgccgcatc gaacgctacg tgctgttcgc
     6781 catccttgcg gaattctgcg tgcagctggt caatgcaact ttcatgaacc tgcagacgct
     6841 gtacatgaaa gcggaaggtt accacgatgc agagatcgcg gattttattt cctggcgttt
     6901 cgccggcgtg ctcgttttcg cattcccgct gggcatgttc atccgcggaa aaaaagtgcg
     6961 gcctttattc cggatctcgt ccgcgggcgt accgcttttt gcgttatgca ttttgtggtg
     7021 cgtgcatttc catcatgaca acctgctcat cgtttctcaa tggatgtggg gaacctgctt
     7081 cacgttcatc cagatcccga tgatcccgtt catcctgcgc aactgcagcc cggagaaccg
     7141 gacggccgcc atttcgctca gctattccac gtggagcctg gcggggatca gcagcgcggt
     7201 gttcgtggct gtcaccaatc acatcaaccc ggttcttttc agcgaacgca acctcatggc
     7261 cgcaatttcc gtggcggcgt ttgccggcgt ttattttgtt tcgcgcgtga aaacagaaaa
     7321 agagcccggg gtggaagata agcccgtttc attcgctgcg cagcacgact ggaagctcat
     7381 cttctccgcg ctgttcccgg cgctcatcat cgccacgggg gcgggactga cgatcccctt
     7441 catcaatctt ttcttcagcc gcgtgcacca catggaaacc gacgtgatct cggcctggaa
     7501 tttctgcgcc gctcttctcg tcgccattgc agcgctcacc gttccgaaga tcaaaaaaac
     7561 gatcggttac cgtgttgcgg ttcccaccac gcagacgttc gccgttgttg cgctcgtgct
     7621 catggcgaca acagaactgt acaatcacct cgcgatagcc ggcgtcattg ctgttgcctg
     7681 ttacatcatc cgccagccgc tgatgaatgt cgcaggtccg atgacgacgg aaatatcgat
     7741 gaagtacgtc gggaagaaga accgcgagat cacgagcgcg ctcgctgccg ccatctggtc
     7801 gggaagctgg ttcatcagca cgcgtttcat ttttgcatcg ctgcgcaaca tcgggtggcc
     7861 gtacgtcgac atatttctta tcacggctgc attctacatg tgcggcatca tcctttacgt
     7921 gctgctcatc cgttcctaca acaggagaga gaaagcggga ctgatcacgg aagaatgaca
     7981 accggctttt tccggtagcc ggaaggtttt ttatcttcgt cttgcgcagt ttgtcccccc
     8041 atttctgcat aaataactgc ttatccttga tctgccggaa gaattttatg gcaagggaga
     8101 ctattttttc actacatacg tccaggtgtt tgttacgtcc cttgccgatg cggaaatcca
     8161 tctgcctgct tttctttttt ctttcttcct gcagcctctt ttcccagtcg cctgcgtatg
     8221 tacgctacaa ttctttttcc gggctgccct gcaacgaagt gtacgatgtg atccaggacc
     8281 atctcggttt catctggacg gccaccgatc gcggcatttt ccgtttcgac ggttattcct
     8341 tcgaagcgtt caccactgcg cagggactta ccgacaacac ggttttccgg atctgcgaag
     8401 atttccgcgg acgtatatgg tcgatgccgt tcaacggtga gctgtgctgc attgacaacg
     8461 gccgggtgac cgtttatccg ttcaatgaca cgctgaaaaa tcacctgccg ggtatccgta
     8521 tcgcacgttc gctgaatgtg ctcccatcgg gagaagtgca gatcggctat ctcatccacg
     8581 gcatcgtgtc tgtttcagct gaaggtgttt tcaggagaat ttcaacgagg aagaattaca
     8641 aggcggaata ttatatgatg gacacgggcg gcggactgtt gtatggaatg caggaaacgg
     8701 cgggaatgaa aacgggcacg agactgcagt ttacttcgga agggatgacg gatgagaaac
     8761 tgaatttcga aaagttcagc ggccaccagc agatcgcggg catcaggcgg aggaacgggg
     8821 gctggtgctt tgcattgatg aatgaactgg tggagataag tccgcaaggt acaatgacgg
     8881 agcagccgct tccgaaacag cccttgtgtg tgtacgagga caatgaaaac tgcctctggg
     8941 tgacgatgga cgccggcgga ggcgtgcggc gctaccgtcc cgggaagacg gcctgcggcg
     9001 aaactgtttt cgatacctat tacccgggtg aatcggtgag ccgcatcatg caggatgcgg
     9061 aaggcgcatt ctggatagcg acactttcgc acggactttt ttacgttcct tccgtgcata
     9121 cgttgttgtg gaatatcccg ggtgacgaag aagataaagt tgtttgcctg gcggaggccg
     9181 ggaaggactg cacgttagcg ttgtggaggg acaaaggcct cttccggttg agcggcggca
     9241 attcgcccct ttcaccggtc gtgcttgccg gcgataacaa ttcattccgt tgggtgacgt
     9301 ttgattcgct gcgcagccgg ctcttcattg cggaaaggcg gttcgcggaa gcctgttcat
     9361 ggcccggcct gcaggaggaa aaaaaatggg gactgtcgat acagtcgttc gaaacaggtg
     9421 cgcagggaac attctgctgc gaatcgtggc aggctgcatt ccttgacagc agcgatgaat
     9481 ggaaacagct cggaccgaac ccggacaaac aacgaccgga tgtcatcttc gaagagcggg
     9541 gcgggaagac gtgggtggga agaatggacg ggctctttta tgtttcacac gatacgctgg
     9601 ttcctttcgc ttcgccggat cccgttttcc gcaagcgtgt gagcggcatc tgttcgctcg
     9661 acgacggaac gcttgtggtg gcgacgcaaa gcagcgggat cgcatggata aaaaacgggg
     9721 aaacgcggct gctgtccgat gagaacggca tcataacgga tcatgttctt tcgatcatgc
     9781 cgggaaaggg aaattcgtta tggctgacca ccatctacgg actgtacaat gtgcgcaaca
     9841 ataacggcgt atgggaaaaa tccgtttttc ccgaggtgag ctgctttctc agtaacgacg
     9901 ggcgctgtta tttcacggat aaggatggag atctctggat gtcgaccggc gcgtccgtta
     9961 tccgtttcga tccggatcat tctttcgcca cacactatct tccgccggtt tatttgcgcg
    10021 agatcatagc gaacgggaag gaaatttcca gcgcgcaaaa catcagtctc ccttacgacc
    10081 acaatttcct gcgcatttca ttcgcaggtc ttgcttaccg catgcatggt catctccgtt
    10141 accgttaccg tctttcgcag gaagatccgc aatggacgta tacgtattcc aactcggtgg
    10201 agcttgcgtc gctggggccc gggaattata cattcgaagt gcaggcggaa aatgaacgcg
    10261 gcgtgtggag ctcggctcct gcattctatt cgttcacgat acttccgccg gtttggcaga
    10321 tgtggtggtt caggatagca gcgttcctcg ttctctcggc cattttcttc ctgctgatcc
    10381 gctaccggtt ccggcagctg aagcgccagg cggaattacg cgagcaggcc caggatttcc
    10441 gccagcaggc gctggcgttg cagatgaacc cgcattttgt tttcaacgca ctcaatacga
    10501 tccaggcgta tgtgctgaag gaagataaat cgcgggcgat agaaatgttc tcttcattcg
    10561 cgcagctgct gcggaaaagc ctcgatcacg cgcgcgagcg ttacatcacc ctggccggcg
    10621 aaatagaaat gctcggactt tatttcggta tcgaatcgct gcgtttcgaa gagcggctga
    10681 aatacagcat cacggtggat aagaatatcg acacatccat gacgctcgtc ccggccatgc
    10741 tggtgcagcc cctggtcgaa aatgcgatcc gtcacgggat catgaaccgg aaagcggggg
    10801 gacatgtcga tgtgcgtttt gaaatggagg acgggaagct gttctgtgcg gtggaggata
    10861 acggcgtggg tcgcgcgact gccgcaaagc tccgcgcagg cgatcatcgt tcggcgggaa
    10921 gcgacatcac ggccgaccgt cttcgcgtgc tctgcaaaat gagcggggcg caattttatt
    10981 tttcagtcac tgataaaacc gataaaaacg ggaatgccac gggaacattg gtccgtttcg
    11041 cgatcccttt caccgggaaa aaacaagaag gaatatgaaa cgattaaaag ccctcgtcgt
    11101 cgatgatgaa ccgaatgcag tcgaaggatt tgtccgcctg ctcacgctgt attgccccga
    11161 tatagaaccg gccggaacgg cttcttccgt tggtgaggca ataacgcaga tcaggaagct
    11221 gaagccggat gtcgtgtttc tcgatatcga aatgccggag gaaaacgggt tcaatctcgt
    11281 cgacacgatc cgcgaaaatt cctgctccct tgttttcgtg acggcacatt ccgattatgc
    11341 cgtccgcgca ttccgcaccg acgccgtgga ttacctgctg aaacctgttt caccgcgcga
    11401 gctcaaagca gctgcagagc gtgtcacttc ccgaagggag acgatgaaaa aaacgttacc
    11461 ggatgaagag taccgcatcc gcgtcagcac gacggaaggg atcagcttca ttccctgcgc
    11521 cgaagtgatc agcatcgagg gcagcgggag gtattccatc attcattcgg cggatggagg
    11581 aacgcagctc gtgtcgagga acatcggcga ttttgaaacc gagctggagc cgttcggttt
    11641 ttttcgcgtg cacaaatcct ggctcatcaa ctgccgccac gtcatgcgtc tcgccgccgc
    11701 cgacggggga acgatcgagc tcagcaacgg gaaaaaagtg ctgctctcgc ggcgcaggcg
    11761 gaatgatttc ctgaaaaaga tggaacgctg atcccgtttc ttacgccgat gtcaccggtg
    11821 gttacttcag caatgcagct gcttacgcgg caggtgtgaa tggaattccg ggcgtatagt
    11881 tttggtgaaa aatgtgccgc taaagcggga aaggtttatt caccaaactt ctatccatga
    11941 aaaaaagatt cctgttcaac atcgggtttg cagcgtccct tctttttaac ctgcagcatg
    12001 catctgcctg cgatttttca gatgattttt cttccgcggt accatggacg cagctgggaa
    12061 gcggcgtgca cgtcagcagt gggaattgcg tttacaatcc cgcggtatgc ggatccgacc
    12121 agcgtgtctt ccgttcgctc agcccgacaa cttccatgct ggataacact tcctggacgg
    12181 cgcttgttga ttttatgccg cagaccactt tttcttccgg ggtagcgcac agcatcatca
    12241 gctttacgtc caatacgctt aacccttacc gttacacgtc atccggatcg tatacgccca
    12301 cagatttcat cgagatactt tacagcagcc cgatcaatac cgggttttcc caatggaaat
    12361 tgtacgccag cgcaaaagat ggtgcgaatg cgggccaggt gtcgtccgca ggtatcagtc
    12421 ttccttccct gaacacctgg tactacctcg agcttcagcg cagcggaaac accgttaccg
    12481 taagtgcatt ttccgatccg gcgagaacca ttccgctgag cggttctccg gtttccctga
    12541 cattcaccgg cagcatcacc aacctcgcgt atgtgcagtc cggatctgtg atccagggtg
    12601 ccgcttcgcg caaactgaat gcgctgatcg acaatatttg cgtgacgggt ctcagggccg
    12661 gcggcaactg cgatgtgtgg tctgattata cttcatccac cgggtggacg cagatcaaca
    12721 gcggggtgag catcaccggc ggaaatgtgt tatacaataa tgtcgccgac ggcgggcata
    12781 tccagagaag agtttatgag tcgcttggcg gcgatatcac cggtgcgaat acctggaaag
    12841 cggatttcga tttttacccg acggcagttg gaacgggcgg acccggtcat acgctgtgtg
    12901 gtataacagc cggaacacag gatcctttct gcgatgacag ttacacagct acgaaccagg
    12961 atgcgatcgc agcatgctgg atatcgaaca atcacacgga cgatccttcg acgttcatgc
    13021 ttgatggccg tgcgaaggat ggaaacggtg cgtggtcaac gtccgcggga attctgcttt
    13081 ccctcggcac gcattattac ataacgctcg aacgtcttga tgaattcaac ggacgcatca
    13141 gcgcttttct ggacgcggga agaacgatcc atgcgccagg ttctccgcag tgttttacaa
    13201 tcccgcccgg cgttttcaat cttgattacg tacagcacgg atcaattccc tggggtggtg
    13261 acgaccgtgc gcttaccgct accgtagata atttttgcct gaaggcggat tacctcaacc
    13321 cgggaacagt cagctataac ggacctctca gtttctgcgc gtcgggaact ccttccgtga
    13381 tatcatccga aactcccgcc accgggggca actggacctg gaactaccag tggcagcaat
    13441 cggtgaattg taacggcgtg tggaccgaca ttgccggtgc aaccggaact tcctatacac
    13501 catcgtcgat ctcgacgtca acctgctacc ggcgtgccgc gctcgggccg tgcggcgttg
    13561 cggtttattc caatgttatc ggcatactcg tataccagcc gctttccgca aacatcactt
    13621 ccacggagac cggcgatcat tgtacggctt cgtatacgct taccgcaaac ccgagtaatc
    13681 cgacaggtgt aacgtatctc tggaacaccg gcgctaccac ccaatcaatc gtggtgggta
    13741 atccgaattc accgacaaca tattcagtca cggtaacgag cccgtgctat accgcaactg
    13801 caagttatgt ggtatatccg tgttatcatc cttccggtgc attcccgacg atctccgcaa
    13861 attcatggtt cgaagtgaac tggagcggtg gttgtaccgg ttcgtgctac ctccagttta
    13921 tcgatcccgc ttacccggtc ggcggtggcc ctgcgtatgg agtgacccag gctgagctgc
    13981 tggtgttcga tcgttggggg aacatgcttt acgaccaggt ttatgttccc agtccgccct
    14041 gcgatggtct ctggaacggc cagatcatct ggaatggtat ttacatggga tacccggtgg
    14101 tagacggcga ttatgattat gtgctgaaac gtacgaactg ttcgggaaat gatgaggtaa
    14161 gaggatccgt tgtcgtgctg ggaacggcac gtatggccag ccatgatgcg gatacaactg
    14221 tcgcgacata tacgcctcca ttatctgtcg tatatccgaa tccttccgct ggcgttttca
    14281 acgtcgatct tttgccatcc ataaataacg tcgatgtaac gatggaagt
//
LOCUS       DASTSF010000192         6115 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_936029,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000192 DASTSF010000000
VERSION     DASTSF010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 6115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6115
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_936029"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..881)
                     /locus_tag="VFU15_05535"
     CDS             complement(<1..881)
                     /locus_tag="VFU15_05535"
                     /inference="COORDINATES: protein motif:HMM:NF026253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HEU4717269.1"
                     /translation="MRIIFISFLLFSFFVKGSAQHPGQAIPFRDGNAWGFADSTGKII
                     SPCIYDYIDARVDSSFFVVGLAGKYGVVNDSGRLIVSAEYDRIEFDHAFIRIVTGNKC
                     GLAGRSGKIVLPVVYDVIEFSGERPGLAIVNRYGKWGLASVLTGKEIIPCMADDDSPL
                     WRGDDIIVLSQGNKYGACNQNGKLLVPFRYMYVQLAFTDSPTILAYTKKALDVYDTTG
                     HLLVKTGRDEYVGRNDSLLFFDGRKGLYVLDRKGRKRLPAKGQVMWTGYLSMQNYFQV
                     HGRRPGTYGISAANGKPV"
     gene            989..1573
                     /locus_tag="VFU15_05540"
     CDS             989..1573
                     /locus_tag="VFU15_05540"
                     /inference="COORDINATES: protein motif:HMM:NF039496.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEU4717270.1"
                     /translation="MSQSASTRITAAYAKMPENIRAIVEKVRAIIHKAEPAIEENWKW
                     GPAFEKNGLAMGLWGFKKHVSFVFYRGAEMSDRHKLFNDGHDNAHNRMIKFTSIKEVN
                     EKKLADYVKEAVKLNASGAKKEAKTERKIDVPEELQKWFAKNKKAKTFFDGLSFTYKK
                     EMTQFISGAKQQETRERRFKRVTESLKKGKKEIR"
     gene            1621..2043
                     /locus_tag="VFU15_05545"
     CDS             1621..2043
                     /locus_tag="VFU15_05545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717271.1"
                     /translation="MKKLIVTFLLLASASFAFAQDTAKKEKPAPQKSPEDRAAHFANH
                     LQKDLGLTDDQKTKVHDLALAKEQKMDALRQQHQGDDPKSWSADRKKVHDDFITGMKG
                     VLTPDQYNKWEAEKKQHQQQHQQQRPHHKPGADSTQGK"
     gene            complement(2111..2755)
                     /locus_tag="VFU15_05550"
     CDS             complement(2111..2755)
                     /locus_tag="VFU15_05550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717272.1"
                     /translation="MKTFFLPLALLFLFTGCGNEKNTDTNSDKPLTGKDSLKKEITEN
                     LPPPTEIRQFSWFYSMFAAAATMDGGDTIFDHYVSPEHGLWIISSEGAMPHFTNVKSI
                     NDYKRSDGTKLLPMDKDRMLAAPKEEELPSVDCNSKTFWNKDGCFTREENKFKEEKIW
                     QYAGLTNDKEKEVEALAGTISRTVINTQTYRFYFSLINGSWYLTFLDIRKPCEA"
     gene            complement(2781..4049)
                     /locus_tag="VFU15_05555"
     CDS             complement(2781..4049)
                     /locus_tag="VFU15_05555"
                     /inference="COORDINATES: protein motif:HMM:NF024241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU4717273.1"
                     /translation="MRNWLTRTVLLLSFVSLLNDMSSELLYPVLPLYMAGIGYGAFWI
                     GVLEGVAEATAGLTKGWFGEWSDKTGRRLPFVRFGYSLSALCKAFLPLFANAFWAVTM
                     RAGDKVGKGFRTGARDAMLADESEGKNRGKVFGLHRAMDTAGAVFGPMMAFAWIWFFG
                     DSNYVPLFYWSLLPGGLAILLLFLIRDGKRKAKEGKHVSLFSSFGYWKKSTKQYRKLV
                     SVISFFTFFNSSDMFILLLAGKIVKEQHYFLPFNSNQGLITVLLYIFYNLVYSLFSYP
                     SGSLSDKFGHGKMLRFGYTFFAIAYGILALFAVHVIDNIYILPVAFVFYGLYSAFTEG
                     NSRAWISTVCNEGDKGVALGLFAGISSIAALGASVLAGIAWEGLGAGIVFILPACAAF
                     ISVIFLTFGFKAPGKDSVEMIRQPEHEDRS"
     gene            4150..4500
                     /locus_tag="VFU15_05560"
     CDS             4150..4500
                     /locus_tag="VFU15_05560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717274.1"
                     /translation="MKKLAFIFGFTALFAIGLSAQSSAPAGSKNPSSSSTPPPSKTTV
                     TKPAPAPTGSATTTAAPADQTRNTGSTGTGANTKPAKRAPRKAGSVSAKPKAAPKTAS
                     KNSAQLASPKDKLK"
     gene            4660..5259
                     /locus_tag="VFU15_05565"
     CDS             4660..5259
                     /locus_tag="VFU15_05565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717275.1"
                     /translation="MKSPKIKPVREPGKLVEPWITWHAFAEKTGGETKEIPGSRFRNN
                     NTFSITLKKNRETIELIWGGNPQNNARASWTVTTLRFGLNDPAPSLNIYRRSLIDSFT
                     GLFRRSSRMESTALLKKLCVIRAGQPLKLPAGVLALLEKAFTGNEPDFFVLTGKDDED
                     FLLIKNFSLLTSGEDLSLFHELGNELARFLRNETGPGND"
     gene            5293..6015
                     /locus_tag="VFU15_05570"
     CDS             5293..6015
                     /locus_tag="VFU15_05570"
                     /EC_number="6.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011585151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphthine--ammonia ligase"
                     /protein_id="HEU4717276.1"
                     /translation="MNLPKAIFCWSGGKDSALCLHKVLSGKMYDVKYLLTTVNDEFRR
                     ISMHGVREELLEAQAASVGIPLITVRVKEGTNDEYEKQMEAALLKAKAEGIGHVIFGD
                     IFLEDLRVYREQNLAKVDLQAVFPLWKQDTSALLREFVQLGFRSVLCCTNDAFLGEEW
                     VGKEIDGNFGEQLPANADPCGENGEYHSFCYDGPLFSRKINFERGEKIYRPLEVKTTD
                     DCTLPSENRTKGFWFIDLLPGK"
BASE COUNT         1641 a         1707 c         1507 g         1260 t
ORIGIN      
        1 accggtttgc cgttcgccgc gcttattccg tatgtccccg gcctgcgacc gtgcacctgg
       61 aaataatttt gcatactcag gtaaccggtc cacatcacct ggcctttcgc gggaagacgt
      121 ttccgcccct tgcgatcgag cacatacagg ccttttcgtc catcgaaaaa caaaagagaa
      181 tcattgcgcc cgacatattc atcacgaccg gtttttacca gcaggtggcc ggttgtgtcg
      241 tagacatcga gcgctttttt cgtgtacgca agaatagtgg gcgaatcggt gaaggcaagt
      301 tgcacataca tgtagcggaa cggcacgagg agttttccgt tctggttaca ggcgccgtat
      361 ttattcccct ggctcaatac gataatatcg tcgccgcgcc acaacggtga atcatcgtcg
      421 gccatgcagg gaataatttc cttcccggtc agaacggatg ccagtcccca ttttccgtaa
      481 cgatttacga tcgccaggcc gggccgttca ccggaaaatt cgatgacatc gtaaacgaca
      541 ggcagcacga tcttcccgct gcggccggca agaccgcatt tgttgcccgt cactattcgt
      601 atgaacgcat gatcaaattc aatcctgtcg tattccgcgg aaacgatcag cctgccggaa
      661 tcattgacga ctccatattt tcctgcgaga cccacgacaa aaaaagaaga atctaccctg
      721 gcgtcgatat aatcatagat gcacggagaa attatttttc ccgtgctgtc ggcaaatccc
      781 catgcgtttc catcgcggaa aggaatggcc tgaccgggat gctgcgcgct ccctttcacg
      841 aaaaaagaaa acagcaggaa ggaaatgaaa atgatgcgca tgaaaagggc tcccgcaaag
      901 ttagaattcc actcaggaat aacgcctgaa ccctgaatgg ttacaatgtt tcctcaaaaa
      961 cgaacgatgt catttcctat ttttgagcat gagccagtct gcatccaccc gcatcaccgc
     1021 tgcttacgcg aagatgcccg aaaacatccg cgccatcgtt gaaaaagtgc gcgccatcat
     1081 ccacaaagcc gaacctgcca tcgaggaaaa ctggaaatgg ggaccggcgt tcgaaaagaa
     1141 cgggcttgcc atgggtttgt ggggattcaa aaagcacgtg tcgtttgttt tttaccgcgg
     1201 cgcggaaatg agcgacaggc acaagctgtt caacgacggt cacgacaacg cgcacaaccg
     1261 catgatcaaa ttcacgtcga tcaaagaggt gaatgaaaaa aagctggcgg attacgtgaa
     1321 ggaagccgtg aagctgaatg cctcgggtgc aaaaaaggaa gcgaaaacag aacggaagat
     1381 cgatgtgccg gaagagctgc agaagtggtt cgcgaaaaac aaaaaggcga aaacgttctt
     1441 cgacggactt tcattcacgt acaaaaagga aatgacgcaa tttatttccg gcgccaaaca
     1501 acaggaaacg cgtgaacggc gttttaaacg ggtaaccgaa tcgttaaaaa aaggcaaaaa
     1561 agaaatccgc tgagaaaggc ggaagacttt ttgatcgtct aacccacaaa aatcaatacc
     1621 atgaaaaagc tcattgtcac cttcctcctg ctcgcttccg cttcatttgc gttcgcgcag
     1681 gacaccgcca aaaaagaaaa gcccgcaccg cagaaatccc cggaagacag ggctgcgcat
     1741 tttgcgaacc atctccagaa agacctcggc ctgacggatg accagaaaac gaaagtccat
     1801 gacctcgccc ttgcgaaaga gcagaaaatg gatgcgctcc gccagcagca ccagggcgac
     1861 gacccgaaat cctggtcggc cgatcgcaaa aaagtgcacg acgattttat caccggcatg
     1921 aaaggtgtgc tcacgcccga ccagtacaac aaatgggaag cggagaaaaa acagcaccag
     1981 cagcagcacc agcagcaacg tccgcatcac aaaccagggg cggacagcac gcagggtaaa
     2041 taaaaagaaa ccgggttcgt cccggttttt ttaattgtga gaagcgtatc tcacacacgc
     2101 aaaaaaacag ctacgcttca caaggtttcc ggatgtcgag gaacgtcagg taccaggaac
     2161 cgttgataag ggaaaaataa aaccggtacg tctgcgtgtt gatgacggtg cgcgagatgg
     2221 ttcccgccag cgcttccact tctttttcct tgtcgttcgt gagcccggcg tactgccaga
     2281 tcttttcttc tttgaattta ttctcctcgc gcgtgaagca gccgtccttg ttccagaaag
     2341 ttttcgaatt gcagtcgacc gaaggaagtt cctcctcttt cggggcggcc agcatccggt
     2401 ctttgtccat gggcagcagc ttcgttccgt cgctgcgctt ataatcgttg atgcttttca
     2461 cattggtaaa atgcggcatc gctccttccg agctgatgat ccagaggccg tgctccgggc
     2521 tgacataatg atcgaatatc gtgtcgccgc cgtccatcgt tgcggcagcc gcaaacatgc
     2581 tgtagaacca gctgaactgt ctaatttccg tcggcggggg aagattttcc gtgatctctt
     2641 tcttgagcga atctttcccg gtcagcggct tgtccgaatt cgtgtccgtg ttcttttcat
     2701 ttccgcaacc cgtaaagaga aaaaggagcg caagcggtaa aaaaaatgtt ttcatgccgt
     2761 tcggatttat tcgccgcacc tcaggaacga tcttcatgct ccggctgccg gatcatttca
     2821 acggaatctt ttcccggtgc tttgaatcca aaagttagga aaatcaccga aataaacgcc
     2881 gcacaggcgg gaagaatgaa aacaataccg gcgcccaaac cttcccaggc gatcccggcc
     2941 agcacgcttg caccgagcgc agcgatgctc gaaattcctg cgaacagccc cagcgcaaca
     3001 cctttatccc cctcattgca caccgtgctg atccacgcac gggaatttcc ttccgtgaac
     3061 gcggaataaa gtccgtaaaa aacaaacgcg accgggagga tgtatatgtt gtcaatcacg
     3121 tgaacggcaa aaagcgccag tattccatag gcgatcgcaa agaacgtata cccgaaacga
     3181 agcatttttc catgaccgaa tttatccgaa agcgatccgg agggatacga gaaaagcgaa
     3241 tacaccaggt tgtaaaaaat gtagagcagg acagtgatga gtccctggtt ggaattgaac
     3301 ggaagaaaat aatgctgctc cttcacgatc tttcccgcca gcagcaggat gaacatatcg
     3361 gagctgttga agaacgtgaa gaaggaaatg acggaaacca gcttccggta ttgcttcgtg
     3421 gattttttcc agtaaccgaa agaagaaaaa agggaaacgt gctttccttc tttcgccttg
     3481 cgtttgccgt cgcggatgag aaagagcagc aggatggcca gtccgcccgg cagcagcgac
     3541 cagtaaaaca gcgggacgta attcgaatcg ccgaaaaacc agatccacgc gaacgccatc
     3601 atcggtccga acacggcgcc tgccgtatcc atcgcacggt gaagcccgaa cactttcccg
     3661 cggttcttcc cttcgctttc atccgcgagc atcgcgtcgc gtgcacccgt gcggaaacct
     3721 tttcccactt tgtcgcccgc acgcatcgtc accgcccaga acgcattcgc gaacaaagga
     3781 aggaacgctt tgcaaagcgc gctcagcgaa tacccgaaac gcacgaaggg cagacgcctg
     3841 ccggttttat ccgaccattc cccgaaccag cccttcgtca gcccggccgt cgcctccgcc
     3901 acaccttcca gcacaccgat ccagaatgcg ccgtaaccga ttcccgccat gtacaaaggc
     3961 agcacaggat acagcagctc gctcgacata tcattcagga ggctgacgaa cgagagcagc
     4021 aaaacagtac gggtaagcca gttccgcaaa atttgagtac tttaacgtga gaagcgctaa
     4081 agttacgcgg cctccgccga ctttggcacc aaacttggat catccgcatt aaccaaaaca
     4141 ttcacagcaa tgaaaaagtt agcattcata ttcggattca ccgccctttt cgcgatcgga
     4201 ctttccgcgc agtcttctgc accggcaggt tcgaagaacc cgtcctcttc ttccacgcct
     4261 ccgccgtcca aaaccacggt gaccaaaccg gcgcccgcac ctacgggttc cgccacgact
     4321 accgcagctc cggcagacca gaccaggaac acgggttcaa ccggaaccgg tgccaataca
     4381 aaaccggcca aacgcgctcc gcgcaaggca ggatccgttt ccgcaaaacc caaggcagcg
     4441 cctaaaaccg ccagcaagaa ttccgctcag ctcgcttcac cgaaagacaa gctgaaataa
     4501 cggtcccgcg ttccatcaca ttcccggcgg cgttctgcaa ctgaacgccg tttttttttg
     4561 ccgttacctc gacgaaacag cacaatctcc tttttttcac cgcaattatg cgcagttcag
     4621 ccagggccgt tctatatttc gtgaacagcc ccctggcgaa tgaaatcccc gaaaataaaa
     4681 ccggtacgcg aacccgggaa actggtcgaa ccctggatca cctggcacgc atttgcggaa
     4741 aaaaccgggg gagaaacaaa ggaaattccc ggatcgagat tccggaacaa caacacattt
     4801 tcgatcaccc taaaaaaaaa cagggagacg atcgagctca tctggggcgg caacccgcag
     4861 aataacgcac gggcctcatg gactgtgaca acactgcgtt tcgggctgaa cgaccctgca
     4921 ccttccctga atatttaccg gcgttcgctc attgattcat tcaccggcct gttcaggcgg
     4981 tcatcacgga tggaaagcac ggcgctgctg aaaaaactgt gtgtgatacg cgccggtcag
     5041 ccgctgaaac ttcccgccgg cgtgcttgcg cttctcgaaa aagcgttcac gggaaacgag
     5101 cccgattttt tcgtactcac cggaaaagac gacgaagatt ttctcctcat caagaacttt
     5161 tcactgctca cctcgggaga agatctgtcg ctctttcacg agctgggaaa tgagcttgcg
     5221 cgttttctcc ggaatgaaac cggcccggga aacgattaat ttcgtcccgg cgccaattgg
     5281 cggcacaaga ccatgaacct tcccaaagcc atattctgct ggagcggcgg aaaagattcc
     5341 gcgctgtgcc tgcacaaagt tctttccgga aaaatgtacg acgtaaaata cctgctcacc
     5401 accgtgaacg acgagttcag gcgcatatcc atgcacggcg tacgggaaga attgctggaa
     5461 gcgcaggctg catcagtggg cattccgctc atcacggtgc gcgtaaagga aggaaccaac
     5521 gatgaatacg aaaagcagat ggaagccgcg ctgctgaaag cgaaagcgga agggatcggt
     5581 cacgtcatct tcggtgatat tttcctcgaa gatcttcgcg tttaccgcga gcagaacctc
     5641 gccaaagtgg acctgcaggc tgtttttccc ttgtggaagc aggacacgtc agcgctgctc
     5701 cgcgaattcg ttcagctcgg tttccgcagc gtgctctgct gcacgaacga cgcgttcctc
     5761 ggcgaagaat gggtgggaaa agagatcgac ggtaatttcg gggagcagct tcctgcaaat
     5821 gcagatccgt gcggggagaa cggggaatat cattcgttct gctatgacgg accgctgttc
     5881 agccggaaaa taaattttga gcgcggcgaa aagatctacc gcccgctgga agtaaaaacc
     5941 acggacgact gcacgcttcc ttcggaaaac cgcacgaaag gattctggtt catcgacctc
     6001 ttgcccggta aataattcgt gatatgtacc tgcttccatc cacccatgaa aaaagttctg
     6061 ccgctctttc ttttcgtcat cgcattcgca gctggcaaag cgcagaacat cgtcc
//
LOCUS       DASTSF010000193         2874 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_267296,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000193 DASTSF010000000
VERSION     DASTSF010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2874)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2874
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267296"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(557..1606)
                     /locus_tag="VFU15_05575"
     CDS             complement(557..1606)
                     /locus_tag="VFU15_05575"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF015131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HEU4717277.1"
                     /translation="MKNRKTAIVIFFAGIVALLLSFSPGNDYFAITAKDVELKIPKGF
                     PKPGYDFKKNPLTPEKFVLGRKLFYDPILSLDSTISCASCHQRIAAFAHIDHPISHGI
                     YGLFGKRNVPALQNLIWKDAFMADGGVNNLEVQPVNPITNPVEMNESLAHVVMKLKRN
                     PVYVQLFATAYGDTLINSEHILKALAQFTGLMISADSRYDKFTRGEDTLSAAEMRGLK
                     LFRAKCADCHREPLFTDNSYSSNGIAPDTSLHDAGRMAITGVASDEYKYKVPSLRNIA
                     MTYPYMHDGRFRSLQQVLDFYAAGKYFTANADPRLKKSAGMTSDEKSDIIAFLQTLTD
                     KTFLYDRRFADPNMQ"
     gene            complement(1596..2339)
                     /locus_tag="VFU15_05580"
     CDS             complement(1596..2339)
                     /locus_tag="VFU15_05580"
                     /inference="COORDINATES: protein motif:HMM:NF042462.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbnP family protein"
                     /protein_id="HEU4717278.1"
                     /translation="MKNAAAAILFSFLLMGMGPQKAVNAGLDINIENRVGEKALVLDT
                     VHYKNSLGQDFTVTNFKYYLSNFSLKTGDGRTYNSPDYFLVKADDESTWKIRLRGIPP
                     GKYESLSFLIGVDSLHNCSGAQSGALDPANGMFWAWNTGYIFLKFEGKAESSKSPGHI
                     FEYHIGGYKAPANCIRKVTIDLSKMPVVAQAGKKSTMQLIAQAMEILVNPVAIDFSKL
                     SSVSDFHNSPMIADNYADMFSAGNVVNEK"
     gene            complement(2443..>2874)
                     /locus_tag="VFU15_05585"
     CDS             complement(2443..>2874)
                     /locus_tag="VFU15_05585"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEU4717279.1"
                     /translation="TYAYFVTIDASLNPVYPFVIGPTYYGTVQSGNTGPTGGHVTITE
                     STTVYTGATGINDAAAHTIGATIVPNPVQDYAFVYMDATSANNVTGRLYNSTGQLIST
                     TENMQPSIAYSLDMTACPPGVYIFVMESGDRKFTQKIVKTK"
BASE COUNT          689 a          740 c          746 g          699 t
ORIGIN      
        1 cgggcaggtg atgaccactt cggttccgca gaacgattca acgacgatga atacggaaaa
       61 tctcgcgaac ggtgtttacc tcgtgcgtgt gtacagcggt aattcggtaa cgacgcaaaa
      121 gctcacaatt acgcgataga ggaatttctc caggagtaaa gcgcattccc cgcgcaaaaa
      181 ggtcccggcg gcaaaccggg atcttttatt ttatgaaggt tggatccgtt ttttgaaaag
      241 tctcaccacg aagcgcgcaa agaacaccga gctgtgttaa aacaaatcct gcaagacgca
      301 atgacgcgga agaaaatctc tgtgcttttc agtgaaacaa cctccgtgaa cctccgcgtt
      361 aagaaaacca cgaagtgcac aaagctgcgc acaaagaaca caaagctgtg ctaaaacaaa
      421 tcctgcaaga cgcaatggcg tgaaagaaaa tttctgtgtt cctcagtgaa acaacctccg
      481 tgaacctctg tgttaagaaa accacgaagc tgcgttaaag caattcccgc gaacgcgcga
      541 agagaaaact ccttcactat tgcatattcg gatccgcgaa gcggcggtcg tacaaaaaag
      601 ttttgtccgt cagtgtttgc aggaaagcga tgatatccga tttttcatca ctcgtcatcc
      661 cggcggattt tttcaggcgc ggatcggcat tggctgtaaa atattttccc gccgcgtaga
      721 aatccagcac ctgctgcagg ctgcggaaac gcccgtcgtg catgtacggg tacgtcatcg
      781 cgatgttgcg gagcgaagga actttgtatt tgtattcatc cgacgcaaca ccggtgatcg
      841 ccatcctgcc cgcatcatgc agcgacgtat ccggtgcgat gccgttgctg ctgtacgaat
      901 tatccgtgaa caaaggttcc ctgtggcaat ccgcgcactt cgcacggaac aatttcagcc
      961 cgcgcatttc ggcggcagaa agcgtatcct ctccccgcgt gaatttgtca tagcgcgagt
     1021 ctgccgagat catcagtccc gtgaactgcg ccagcgcttt caggatatgt tcggaattga
     1081 tgagcgtatc gccgtaagcc gtggcgaaca gctgtacgta aacaggattc cgtttcagct
     1141 tcatcaccac gtgagcgagc gattcattca tctccacggg gttcgtgatc gggttgaccg
     1201 gctgcacttc gagattgttc acgccgccgt cggccatgaa cgcatctttc cagatgaggt
     1261 tctgcagcgc aggcacattc cgttttccga aaaggccgta aatgccgtga ctgatcgggt
     1321 gatcgatgtg agcaaatgcc gcgatgcgct ggtggcagct ggcacagctg atcgtgctgt
     1381 cgagggaaag gatgggatcg taaaataatt tccggccgag gacgaatttt tccggcgtca
     1441 gcggattttt tttgaaatcg taacccggtt tcggaaaccc tttcgggatc ttcagctcta
     1501 cgtctttcgc ggtaatggca aaataatcgt tgcccggact gaagctgaga agcaatgcga
     1561 caataccggc aaagaaaata acgatcgctg ttttcctatt tttcattgac aacatttccc
     1621 gcgctgaaca tatctgcata attgtccgcg atcatcgggg aattatggaa atcggagacg
     1681 gaggaaagct tcgaaaaatc gatcgcgacc gggttgacga gaatttccat cgcctgcgca
     1741 atgagctgca tcgtggattt tttcccggcc tgcgccacga ccggcatttt cgaaaggtcg
     1801 atggtaactt tccggatgca gttggcaggc gctttgtacc cgccgatatg gtattcgaaa
     1861 atgtgccccg gcgatttcga gctttccgct ttcccttcga acttcaggaa gatatagccc
     1921 gtgttccagg cccagaacat gccgttggcg ggatcgagcg ctcccgactg cgcgccgctg
     1981 cagttgtgca ggctgtccac gccgatcagg aagctcagcg attcgtattt gccgggagga
     2041 ataccgcgca gccggatttt ccaggtcgat tcgtcatccg ctttcacgag aaaatagtcg
     2101 ggtgaattgt acgttcttcc gtctcctgtt ttcagcgaaa aattgctgag gtaatatttg
     2161 aaattcgtca ccgtaaaatc ctgcccgagc gaatttttat aatgcaccgt atccagcacg
     2221 agcgcttttt cacccacgcg gttttcgatg ttgatatcca gtcccgcatt gacggctttc
     2281 tgcggaccca ttcccatcag gagaaaagaa aaaagaatgg cagcagctgc atttttcatg
     2341 tcgttcggat tttctgtgaa ctgttaccgt ccccgagcag caaaaagcaa aagcacgaag
     2401 cgcttgcgac tccgtgccct gcagaaaacc gtttgcccaa acttatttcg ttttgacgat
     2461 cttctgcgtg aatttcctgt cgccggattc catcacaaaa atataaacac cgggcggaca
     2521 tgcggtcatg tcgagcgaat aagcgatgct cggctgcatg ttttccgttg tgctgatgag
     2581 ctggcccgtt gaattgtaaa gtcttcccgt aacgttgttc gcgctcgtcg cgtccatgta
     2641 aacgaatgca tagtcctgca cggggttcgg aacgatcgtc gcgccgatag tgtgcgcggc
     2701 tgcatcgttg atcccggttg ccccggtata taccgtcgtc gattccgtaa tggtcacgtg
     2761 cccgccggtg ggacctgtgt ttccggattg caccgttccg taataggtag gcccgatcac
     2821 aaacgggtaa acagggttca ggctggcatc gatggtgacg aaatacgcgt atgt
//
LOCUS       DASTSF010000194         2621 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1492104,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000194 DASTSF010000000
VERSION     DASTSF010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2621
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1492104"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..523
                     /locus_tag="VFU15_05590"
     CDS             <1..523
                     /locus_tag="VFU15_05590"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEU4717280.1"
                     /translation="LIVVSKSLGNDKSFMPNIRDYLMSGKIDSARELCRAKDTPIAHV
                     IEKGISRLGKPTREVSEAMESAGQQEIARVEKNLHFLSLVSRIAPMIGFIGTIIGVIT
                     IFHKIGLSGDISIKGISNGLYIKMFSSAAGLLVGVIAFLFYHVLNAMVDNLAKKIEHS
                     SSAFLDVINEPGN"
     gene            553..957
                     /locus_tag="VFU15_05595"
     CDS             553..957
                     /locus_tag="VFU15_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015031068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEU4717281.1"
                     /translation="MKLRRKNKEGAEVFTDSLNDIMFFLLLFFIIISTLANPNVIKME
                     LPSSKSVSPLTDGKVLLQIDANHNYYVNNVPITIDQMETALLHETQAKGTNSVILSLD
                     KSLTVQDLADVLQIGSKLGLKMVLSAKPSNTQ"
     gene            954..1802
                     /locus_tag="VFU15_05600"
     CDS             954..1802
                     /locus_tag="VFU15_05600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717282.1"
                     /translation="MTNAVAIPFAMNGNERKARRIAWIVTVIVTIALLLVMILVKLVT
                     PIPAIPPDPEVMTVEVGLVDGSGGDAAVQGGGSSGNTGIPGSQDADASAHDVKNPADN
                     GSVTDNSSDNPPAANTTHTSNNNQPTASNDLLAALNNWKKNKGAASINIGGNGSGDPY
                     TGGLGNGSGSDIGPNNGGDPGTGTPGGHQGTDPSGDGHRYRHIVSKPDIVNPTQEEGK
                     VVVNVYVDRNGAVKKAEVNPNGTTTLNAVLRSTATQSAYQIKFDADPLGPELLVIPID
                     IYFTLK"
     gene            1858..>2621
                     /locus_tag="VFU15_05605"
     CDS             1858..>2621
                     /locus_tag="VFU15_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020599234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEU4717283.1"
                     /translation="MSRNRYAETLDYLYSRLPMFQRVGPAAYKADLSNTIAICEALHH
                     PEKKFRSLHIAGTNGKGSTSHLLAAVLQEAGYKTGLYTSPHLKDFRERIRINGKMIPK
                     NYVVDFVQKNKKLFEKTEPSFFEWTVGLAFEFFAEKKVDIAVIETGLGGRLDSTNVVT
                     PVCSVITNISYDHTNLLGNTLRKIAKEKAGIIKKNIPVVIGETQDEVKSVFLLKAKKE
                     NAPLRFADEEYEVKNFRHAKKTSPETIASVYAGKNRV"
BASE COUNT          711 a          716 c          666 g          528 t
ORIGIN      
        1 ccttatcgtc gtttcgaaat cgctcggaaa cgacaagagc ttcatgccga acatccgcga
       61 ttacctgatg agcgggaaaa tcgattccgc ccgtgaactt tgccgggcga aagacacacc
      121 catcgcgcac gtgatcgaaa aagggatcag ccgtctcggg aagccgacgc gcgaagtatc
      181 ggaagcgatg gaaagcgcgg gacagcagga gatcgcacgc gtggaaaaaa accttcattt
      241 cctcagtctt gtttcgcgca ttgccccgat gatcggattt atcggaacga tcatcggtgt
      301 gatcaccatt ttccataaga tcggtctctc gggcgacatc agcatcaaag gaatttcaaa
      361 cggtctttac atcaagatgt tttcttccgc tgccggcctg ctcgtcggcg tgatcgcatt
      421 cctgttctac cacgtgctga acgccatggt ggacaacctc gcgaaaaaga tcgaacacag
      481 cagctccgcg ttcctcgatg tgatcaatga acccggaaac tgattttgcc tgtcactttt
      541 tgaacgcaag acatgaaact ccgcagaaaa aataaagaag gtgcagaagt ctttacagac
      601 tcgctgaacg acatcatgtt cttcctgctg ctgttcttca tcatcatttc cacgctggcc
      661 aacccgaacg tgatcaagat ggagctgccc agctcgaaaa gcgtgagccc gctgacggac
      721 ggaaaagtgc tgctgcagat cgacgcgaac cacaattatt acgtgaacaa cgttccgatc
      781 accatcgacc agatggaaac agcattgctc cacgaaacgc aggcgaaagg aaccaacagc
      841 gttattctca gtctcgacaa atcactgacc gtgcaggatc tcgccgacgt gctgcagatc
      901 ggaagcaagc tcggactcaa gatggtgctt tccgcaaaac cctcgaacac acaatgacaa
      961 acgcggtagc catacctttt gccatgaacg gcaacgaacg gaaagcgcgc aggatcgcgt
     1021 ggatcgttac ggtgattgtg accattgcgc tcctgctggt catgatcctc gtgaagctcg
     1081 ttacaccgat tccggccatt cctcccgatc cggaagtgat gaccgtagag gtcggtcttg
     1141 ttgacggaag cggcggtgat gctgccgtgc agggcggcgg ttcaagcggg aacacgggca
     1201 tacccggatc gcaggatgcc gatgccagcg cacacgatgt gaaaaatccg gcggataacg
     1261 gaagcgtgac ggataattct tccgacaacc cgccggccgc gaatacgacg catacttcga
     1321 acaataacca gccgaccgcg agcaacgatc ttctcgcagc gctgaacaac tggaaaaaaa
     1381 ataaaggcgc agcttccatc aacatcggcg ggaacggaag cggtgatcca tacacaggcg
     1441 gactcggcaa cggaagcgga agcgatatag gcccgaacaa cgggggcgat cccggaacag
     1501 gcactcccgg cgggcaccag ggcactgatc catccggtga cggtcatcgt taccgtcata
     1561 tcgtttccaa acccgatatc gttaacccga cacaggaaga aggaaaagtg gtggtgaatg
     1621 tttacgtcga ccgtaacggt gcagtaaaaa aagcggaagt gaatcccaac ggaaccacca
     1681 cgctgaacgc tgttctccgt tctaccgcaa cgcagtctgc ttaccagatc aagttcgacg
     1741 ccgatccgct tggcccggag ctgctcgtta ttcccatcga catttacttt acgctgaaat
     1801 aagcgccgtt cggacgctcc ggattcatct tttacgatgt agttttgcgg acgaaaaatg
     1861 agccgcaacc gttacgcaga gacactcgat tacctctact cgcgccttcc aatgttccag
     1921 cgcgtgggtc cggccgcata caaggcagat ctttccaata cgatcgccat ctgcgaagcg
     1981 cttcatcatc ccgaaaaaaa attccgcagt cttcacatcg caggcacgaa tggaaaaggt
     2041 tccacttctc acctgctcgc cgccgttctg caggaagccg ggtacaagac cgggctgtat
     2101 acctctcctc acctgaaaga tttccgcgag cgtatccgca tcaacgggaa gatgatcccg
     2161 aaaaattacg tcgtcgattt tgtgcagaag aacaaaaaac tgtttgagaa gacggaacct
     2221 tcttttttcg agtggaccgt cgggcttgcg tttgaatttt tcgccgaaaa gaaagtagac
     2281 atcgcggtga tcgaaacggg cctgggcgga aggctggatt ccacgaacgt ggtcacaccg
     2341 gtttgttccg tcatcacgaa catcagctac gatcacacga accttctcgg caacacgctg
     2401 cggaagatcg caaaagaaaa agcgggcatc atcaaaaaga acattcctgt cgtcatcggc
     2461 gaaacacagg atgaagtaaa aagcgttttt cttttgaaag cgaaaaagga aaatgcgccg
     2521 ctgcgttttg ccgacgaaga atacgaggtg aaaaatttcc gccacgcaaa aaaaacttcg
     2581 ccggaaacga tcgcttcggt ctatgcagga aaaaaccgcg t
//
LOCUS       DASTSF010000195         3975 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_489440,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000195 DASTSF010000000
VERSION     DASTSF010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3975
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_489440"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            121..369
                     /locus_tag="VFU15_05610"
     CDS             121..369
                     /locus_tag="VFU15_05610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717284.1"
                     /translation="MANHEQNGHGQSHDHDITEGSRQYYPKGWFIPLVGLVIVAFGFA
                     LLGGFVLNHVGTDKWGKGDGKKEANKTEQVAPAPGDNK"
     gene            513..2933
                     /locus_tag="VFU15_05615"
     CDS             513..2933
                     /locus_tag="VFU15_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEU4717285.1"
                     /translation="MSNVAEKQTALSPGKMEEYRKIVLTDYRIACESREASLTGRKEV
                     LTGKAKFGIFGDGKEVAQIAMAKVFREGDFRSGYYRDQTFMFATDNLTVQEWFAQLYA
                     HTDLRAEPSSAGRQMNGHFATRSLNEDGSWKDLTKIKNSSSDISPTASQMPRLLGLAL
                     ASKHYRSNPDLHADQFRNFSHEGNEIAFGTIGDASTSEGIFWETMNAAGVLQVPMLVS
                     VWDDGYGISVPKKYQTTKESISEALAGFQRTAGKKGYQIFKTRGWDYPHLVDTYEKAA
                     KLCREEHVPVLVHVEEVNQPQGHSTSGSHERYKSKERLEWEAGFDCIKKFREWIASAG
                     IATNEELDTIEKEAKELVKNQRKAAWDAYLAPLREELNEVNGLLASLETQSANAAFLS
                     KVRSTLASTIDPVRKDILTAVRAALRYTRNENLPAREALAGWLKKNQALNFDRYSSTL
                     YSGKAMDVSPLAPVYSAEEKQCDGREILRDNFDAVLTKYPEVCVFGEDSGFIGGVNQG
                     LEGLQKKYGELRVFDTGIREATIIGQAIGMAMRGLRPIAEIQYLDYLLYALQIMSDDL
                     ATVQWRTVGGQKAPVIIRTRGHRLEGVWHSGSPMGMIVHALRGMYVCVPRNMTQAAGF
                     YNTLLAADEPAIVVEPLNSYRMKERTPSNWGEFKVPLGAPEILREGKDITIVSYGPTL
                     RLCAEAANHLAEAGISAEIIDVQTLLPFDVHHAIVDSLKKTNRLLVVDEDVPGGASAY
                     ILREILEVQAGYRFLDSEPRTLTGKEHRPAYGSDGDYFSKPGADDIFEAAYAIMSESD
                     PQKWPGLY"
     gene            complement(2930..>3975)
                     /locus_tag="VFU15_05620"
     CDS             complement(2930..>3975)
                     /locus_tag="VFU15_05620"
                     /inference="COORDINATES: protein motif:HMM:NF025019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HEU4717286.1"
                     /translation="TFKFIASGSAAAALKLKSNESGAGRFTDFVLPPLTFREYLLLTG
                     EEEYAEECFTKFFGKWDDVTEMKNGERLHLNMDKLNVFFIKYINYGGYPEVSLSETIQ
                     KDPQRYIRNDIIDKVLLRDLPSLYGIQDIQELNSLFTYIAYHSGNEMSLDEISKNSGV
                     AKNTIKKYLTYLEAAFLIKIIHRVDNNLRKFKRAHYFKVYLINPSLRSALFSPITESD
                     PFIGNMVETAIYSQLQHMGNSPLYYARWSKGEVDIVSFGIFSTPNWATEVKWSDKYPD
                     NPSELKSLFQFCSMNRMKKAYVTTRSISQRTSLNGLEYEFIPSSLYCYSLGKSILWTR
                     TNQNLFPDYGDVI"
BASE COUNT         1023 a         1038 c         1029 g          885 t
ORIGIN      
        1 gcaggaaaag ccggatttcg cagctccggc ttttccattt ttttgccccg ccggtttttc
       61 cgtgttcgtg aaattcctga aaactcttat acttgtaccc ggaattctaa ccgaacaatc
      121 atggcaaatc acgagcaaaa cggtcacgga caaagtcacg atcacgacat tactgaaggc
      181 agccgccagt attatcccaa aggatggttc attccgctcg tcggactggt gatcgtcgct
      241 ttcggatttg cgctgctcgg tggctttgtg ctgaatcacg tgggaacgga taaatgggga
      301 aaaggtgacg gcaagaaaga agcgaataaa accgaacagg ttgcacccgc tcccggcgac
      361 aacaaataac tgtaccagct tattctactg caggaccttc gggtcctttt tttttgcgtt
      421 gctgcacagc ccgcgccagc gcaggaattc cgcgtttatt tatttaactt taaggccaca
      481 cctttttcgg tcaaacacac aaaccggatt ttatgagcaa cgttgcggag aaacagaccg
      541 ccctgtcccc gggcaaaatg gaggaatacc gtaaaatcgt tctaaccgac tatcgcattg
      601 cctgcgaaag ccgcgaagcc agccttacgg gccgcaagga agtgctgacg ggcaaagcca
      661 aattcgggat tttcggagac ggcaaggaag tggcccagat cgccatggcg aaagttttcc
      721 gtgaaggcga tttccgctca ggctattacc gcgaccagac attcatgttt gcaacggaca
      781 acctgacggt gcaggaatgg ttcgcgcagc tttacgcaca cacggatctc cgggcggaac
      841 cttcttcggc cggaagacag atgaacgggc atttcgcaac gagaagcctc aatgaagacg
      901 gaagctggaa agacctgacg aagatcaaaa attcatcatc ggacatttcg cctaccgcat
      961 cacagatgcc ccggctgctc gggctcgcgc tcgcatcgaa acactatcgc agcaaccccg
     1021 atctccacgc agatcagttc cgcaattttt cgcatgaagg gaatgagatc gcgttcggca
     1081 ccatcggtga cgcttcgaca tcagaaggaa ttttctggga aacaatgaac gcggccggcg
     1141 tgctgcaggt tcccatgctc gtttccgtat gggacgacgg ctacggcatt tccgttccca
     1201 aaaaatacca gacgacgaaa gaaagcattt cggaagcgct cgcaggtttc cagcgcacag
     1261 ctggcaaaaa aggataccag attttcaaaa cgcgcggatg ggattacccg caccttgtcg
     1321 atacgtatga aaaagcggcg aagctctgcc gcgaagaaca cgttccggtt ctcgttcacg
     1381 tggaagaggt caaccagccg cagggccatt cgacttcggg ttcgcacgag cgctacaaat
     1441 cgaaagagcg cctggaatgg gaagccgggt tcgactgcat caaaaaattc cgagaatgga
     1501 tcgcgtccgc cggcatcgcc acgaacgaag agctcgacac gatcgaaaaa gaagcaaaag
     1561 agctggtgaa gaaccagcgc aaagccgcat gggatgctta tctcgctccg ctcagagaag
     1621 agctgaacga agtgaacggg ctgctcgctt cgctggaaac gcagagcgcg aatgccgctt
     1681 tcctttcgaa agtgagatcc acgctcgctt ccaccatcga cccggtgcgg aaagacattc
     1741 tcacggccgt acgcgccgcg ctgcgctata cccgcaatga aaatctcccg gcgcgtgaag
     1801 cgcttgccgg gtggctgaaa aaaaaccagg cgctcaattt cgaccgctac agctccacac
     1861 tgtacagcgg aaaagcgatg gatgtttctc cgctggctcc cgtttattcc gctgaagaaa
     1921 agcaatgcga cggccgggaa atcctccgcg ataatttcga tgccgttctc acaaaatatc
     1981 ccgaagtgtg cgtgttcggt gaagattccg gtttcatcgg cggtgtgaac cagggactgg
     2041 aaggactgca gaaaaaatac ggcgagctcc gcgtattcga cacgggcatc cgtgaagcga
     2101 cgatcatcgg gcaggcgatc ggcatggcca tgcgtggcct ccggcctatt gcggagatcc
     2161 agtacctcga ttacctgctg tacgcgctgc agatcatgag cgacgatctc gccacggtgc
     2221 aatggagaac ggtgggcgga caaaaagcgc ctgtcatcat ccgcacgcgc gggcaccggc
     2281 tcgaaggcgt gtggcattcg ggttcaccaa tgggaatgat cgttcatgcg ctgcgtggaa
     2341 tgtacgtctg cgtgccgcgc aacatgacgc aggcggcagg attttacaac acgctgcttg
     2401 cagcggacga acccgcgatc gttgtggaac cgctgaacag ctaccgcatg aaagaacgca
     2461 cgccctccaa ctggggcgag tttaaagttc cgctgggcgc tccggagatc ctgcgcgaag
     2521 gaaaagatat tacgatcgtg agctatggcc ctacgcttcg cctttgcgcg gaagcggcaa
     2581 accatcttgc agaagcaggc atttccgccg agatcatcga cgtgcagacg ctgctcccgt
     2641 tcgatgtgca tcacgcgatc gtcgattcac tgaaaaaaac gaaccgcctg ctggtcgtcg
     2701 atgaagacgt tccgggcggc gcgtccgctt acatcctgcg tgaaattctc gaagtgcagg
     2761 caggttaccg gttcctcgac agcgagccgc gcacgcttac cggcaaagag catcgtcccg
     2821 cctatggttc tgacggtgat tacttcagca agcccggcgc agacgatatt ttcgaagcgg
     2881 cgtatgcgat aatgagcgaa agcgatccgc agaagtggcc ggggttgtat taaatcacat
     2941 cgccataatc gggaaaaaga ttctgattgg tcctggtcca caagatactt ttgcctaagg
     3001 aatagcaata caggctggag ggaatgaatt cgtattcgag accattcagc gaagttcttt
     3061 gggaaatgga gcgagtcgta acatatgcct tcttcatacg gttcatactg caaaactgga
     3121 aaaggctctt cagctccgaa ggattatcgg gatatttatc agaccatttt acttccgtag
     3181 cccagttcgg agtactgaat atgccgaatg aaacaatatc gacttcccct ttgctccagc
     3241 gtgcgtaata aagcggggaa tttcccatat gctgcaactg cgaataaatt gctgtctcca
     3301 ccatatttcc aatgaaagga tcactttcag tgatgggtga aaacaacgcg cttcgcaagg
     3361 aaggattaat gagataaacc ttgaaatagt gcgcccgttt gaatttcctg agattgttgt
     3421 ctaccctgtg aattatttta ataaggaacg cagcttcaag gtacgtgaga tattttttga
     3481 tcgtattctt tgccactccg gagtttttcg atatttcgtc gagtgacatt tcattgcctg
     3541 agtgataggc tatataggtg aaaagcgaat taagctcctg tatatcctgg atcccgtaaa
     3601 ggctcggaag atcgcgtagc agcaccttgt caatgatgtc attccgtata taccgctggg
     3661 gatctttctg tatcgtttcg gaaagagata cttccggata accgccataa ttgatgtact
     3721 tgatgaagaa cacattcagc ttatccatat ttaaatgcag cctctcaccg ttcttcatct
     3781 ccgttacatc gtcccatttc ccaaagaatt tagtgaaaca ttcttccgca tattcctcct
     3841 ccccggttag cagcaggtat tcacgaaacg tcagcggggg caaaacgaaa tcagtaaatc
     3901 ttcccgcacc ggattcattg cttttcaatt tcaacgcagc tgccgcagaa cccgatgcaa
     3961 tgaatttgaa agtat
//
LOCUS       DASTSF010000196         6545 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_488953,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000196 DASTSF010000000
VERSION     DASTSF010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 6545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6545
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_488953"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            9..1145
                     /locus_tag="VFU15_05625"
     CDS             9..1145
                     /locus_tag="VFU15_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine aminotransferase"
                     /protein_id="HEU4717287.1"
                     /translation="MSSKLPQVGTTIFTVMSKMAVEHKAINLSQGFPDFDCPPELISE
                     VHNAMKAGHNQYAPMPGVMKLREMIAEKTEELYGAKYDPDTEITVTAGATQAIYTAIA
                     SSVRDGDEVIIFEPAYDCYEPAIELNGGKTIYMRLKAPEYSIDWNEVKKVVNHHTRMI
                     IINTPHNPTGAVMTAEDMKQLEKLTKNTDIIVLSDEVYEHIIFDGKKHESVSKFPNLA
                     NRSFIVSSFGKTFHTTGWKTGYCIAPKQLMAEFRKVHQFLVFSCNTAVQYGIAEFLKQ
                     KKHYQELPDFYQKKRDKFIKLLKGSRFTLIPAPGTYFQLLGYDKITNEKDTDYAVRLL
                     KENGVASIPISVFYHKPVYDNMLRFCFAKKDETLEKAAEVLRKV"
     gene            1239..2231
                     /locus_tag="VFU15_05630"
     CDS             1239..2231
                     /locus_tag="VFU15_05630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717288.1"
                     /translation="MPRRIFHLFISLIFIFCLFTRAQAQKDSTLPVKDTMAETPHKDT
                     VKVGVYITSIYNLGFAENDFGIDFWVWYLYKNDSIDPKGTMEVVNAESQEFENGTVEH
                     KGNYTWAVQKCRATIKKNWEISRFPLDDQTLDVYLEETDKDTSELVYVADVENSKIEK
                     SVSLNGWHVSNFTVRPSIYTYNTTFGDPTLSGNSSYARLSISTKIERESKSIFLKLFL
                     GLYVAFLIALAVFFLDGAEIGARTSLAVGALFAAVGNKYIVDSSLPDHASFTIVDKIH
                     DMSFALILISIVFSIISNRFAHKGDSKKSNRFDLACFFGLLVVYVIAHLAIIRP"
     gene            2325..3380
                     /locus_tag="VFU15_05635"
     CDS             2325..3380
                     /locus_tag="VFU15_05635"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase
                     domain-containing protein"
                     /protein_id="HEU4717289.1"
                     /translation="MILDHLHKDQLSRYPGIDIFRGFAIFTMLCANSAAESLAAPHSF
                     LVRIYGSFAAPVFVFLAGFMVALGGRKHPASYFVRRGLEIILTGALIDLFIWRILPFT
                     TFDVLYLTGFGICLGGFFTKLELPAKIALTVLLLAAGPVLQLLFYYEPGVHEFDLTQG
                     FSSLSFPAGWHWWIKSWLFDGWFPVFPWLGLFFAGSLVATNGTVILRKRALFFILGFL
                     LFCGGIAWLYFHRPIEEREGYSELFYPPGLAYLAAACGVILCGLSLLPYLNSHPFFRP
                     LRWLGSCSLFIYILHSALIYFVLDNYFTELSLKNFLLMYLVFAVVNIACAGILFQLKQ
                     QDGWKKVPKVLRFIFGG"
     gene            complement(3431..3919)
                     /locus_tag="VFU15_05640"
     CDS             complement(3431..3919)
                     /locus_tag="VFU15_05640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717290.1"
                     /translation="MSRFLFMAGCLLFTLRAFAQQDTAGNAPRYVVLPGVKENFLLSG
                     YSKYNCWDADSSVIAFAFSHLREGLDSLQKVNEKNFVMAEDFRNMRPFGQYHFQVLGM
                     QDKNKEHLLLFNATCDLRDVNDITWNTYILKVYDGGPCYFSVVYNVTAGRWEMLYFNG
                     TR"
     gene            complement(3916..4461)
                     /locus_tag="VFU15_05645"
     CDS             complement(3916..4461)
                     /locus_tag="VFU15_05645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717291.1"
                     /translation="MASTHHDKHHAYSRDLNIWIDTYDDLFSDFDPRPFSERVISDDF
                     LGELNKLTNERDDFVKVVRFQIPQKNRDEKSEKIIMERLAFDFKRQFAVFSAEVKANY
                     GRGAAMTAAGFLLLACAVLVNAIESQKLWHNFLLIILQPAGWFFVWTGLDRIFFGGKP
                     SKRKRDFYERLAKSKIEFVAI"
     gene            complement(4489..6210)
                     /locus_tag="VFU15_05650"
     CDS             complement(4489..6210)
                     /locus_tag="VFU15_05650"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014031778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HEU4717292.1"
                     /translation="MNLTPNAITVLESRYLLRDADGVLHETPEQLFRRVANAIAMGEK
                     KFGGDPLKYAEAFYELMASLRFLPNSPTLMNAGLEKQQLSACFVLPVEDSLEGIFDTL
                     KEAALIHQSGGGTGFNFSKIRPKNDFIPASGGTASGPVSFMELFDASTERIKQGGKRR
                     GANMGILNCDHPDVEEFISAKTDVNRLRNFNISVGVYDAFLDAVKKKAAWPLIHPNTR
                     KTVREIPAVQLWDSIIDHAWRTGDPGLVFLDALEKANPTPAQGVINCTNPCGEVPLLP
                     FEACNLGSVNLSLFVISREGKPAIDIDALGETVMLAVRFLDNVVEQNQYILPQIEKTV
                     RGNRKIGLGVMGWAEMLILLGIPYASEEAIALGKKLMKFINEVAKEASHALCAERGTF
                     PEWIDSVYAPAVPLRNATRTAIAPTGTIGVIAGTSPSIEPLFALAFRRENVLGHKTLV
                     EENSVVTKLLGEKKLLSEHVLKKMRGEGHVENTDLPAELKLLLKTAQHIGPHWHLAHQ
                     AAFQEFTDNAVSKTINLPNSAAREDIAQIYSKARDLGLKGITVFRDGCKPLQVMNAGT
                     SCEVCAT"
     gene            6339..>6545
                     /locus_tag="VFU15_05655"
     CDS             6339..>6545
                     /locus_tag="VFU15_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007248529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717293.1"
                     /translation="MPPKDLKVAAIQAMLHWEDRDANLKMFDRLLDTITESVDLIVLP
                     EMFTTGFSMSPEKLGKNTDGEALSW"
BASE COUNT         1468 a         1917 c         1631 g         1529 t
ORIGIN      
        1 caggcgctat cagcagcaag cttccccagg tgggaaccac catcttcacc gtcatgtcga
       61 aaatggccgt ggagcataaa gcgatcaatc tctcgcaggg gtttcccgat ttcgattgcc
      121 cgcccgaact gatcagcgaa gtgcacaacg cgatgaaagc cgggcacaac cagtacgcgc
      181 ccatgccggg cgtgatgaag ctgcgcgaaa tgatcgcgga aaaaacggaa gagctctacg
      241 gcgcgaaata tgatcccgat acagagatca ctgtcaccgc cggcgccacg caggccattt
      301 acaccgccat cgcttcttcg gtgcgcgacg gagacgaagt gatcatcttc gagccggcgt
      361 acgattgcta cgaacctgcg atcgagctca acggggggaa aaccatttac atgcgcctga
      421 aagcacccga atattccatc gactggaacg aggtgaagaa agtggtgaac caccacacgc
      481 gcatgatcat catcaacacg ccgcacaatc ccaccggcgc ggtgatgacg gccgaagaca
      541 tgaagcagct cgagaagctg acgaagaaca ccgacatcat cgtgctgtcc gacgaagtgt
      601 acgaacatat catcttcgac gggaaaaagc acgaaagcgt ttcgaaattc ccgaacctcg
      661 ccaaccgcag cttcatcgtt tcttcatttg ggaaaacttt ccacaccacc gggtggaaaa
      721 caggctactg cattgcgccg aaacagctca tggcggaatt ccggaaagtg caccagttcc
      781 tcgtgttctc gtgcaacacc gccgtgcaat acggcatcgc ggaattcctg aaacagaaaa
      841 aacattacca ggagcttccc gatttctacc agaagaaacg cgacaagttc atcaaactgc
      901 tgaagggctc gcgcttcacg ctcattcccg cacccggtac gtattttcaa ttgctcggct
      961 acgacaagat cacgaatgaa aaagacacgg attacgctgt ccgcctgctg aaagagaacg
     1021 gcgttgcgtc cattcccatt tccgttttct accacaagcc tgtttatgat aacatgctgc
     1081 gcttctgctt cgcgaaaaaa gacgagacgc tcgagaaagc ggctgaggtg ttgaggaaag
     1141 tgtaggaatc aaggattacg aaaagcgtct gctccaattc aaaatttcct actttagcct
     1201 gcgtcagcaa tccctcaccg gccacaaccg aacagcttat gccgcgcaga atttttcatc
     1261 ttttcatttc cctcattttt attttttgtc ttttcacacg cgcacaggca caaaaagatt
     1321 ccacgctacc ggtaaaagat acgatggccg aaacgccgca caaagacacg gtgaaagtcg
     1381 gtgtgtacat cacttccatc tacaatctcg gtttcgcgga aaatgatttc ggcatcgatt
     1441 tctgggtgtg gtacctgtac aagaacgaca gcatcgatcc gaaaggcacg atggaggtcg
     1501 tgaacgccga aagccaggaa ttcgagaacg gcactgtcga gcacaaaggg aattacacct
     1561 gggccgtgca gaaatgccgc gccacgatca agaagaactg ggagatctcg cgcttcccgc
     1621 tggacgacca gacgctcgat gtttacctcg aagaaaccga caaggacacc tcggagctcg
     1681 tgtacgttgc cgacgtggaa aattcgaaga tcgaaaaatc ggtttccctg aacggctggc
     1741 acgtgagcaa ttttacggtc aggccttcca tctataccta caacaccacg ttcggtgatc
     1801 cgacgctcag cggcaacagc tcgtatgcgc ggctttccat ctcgacgaag atcgaacggg
     1861 aaagcaagag catcttcctc aaattattcc tcggcctgta tgtcgcattc ctcattgcgc
     1921 tggccgtgtt cttcctcgac ggggcggaga tcggcgcgcg caccagcctc gccgtcggtg
     1981 cgctcttcgc cgcggtgggc aacaaataca tcgtggattc ttcgctgccc gatcacgcat
     2041 cgttcacgat cgtcgataag atccacgaca tgtcgttcgc actcattctc atcagcatcg
     2101 tcttcagcat catctcgaac cgtttcgcgc acaaaggcga cagcaaaaaa tccaaccgtt
     2161 tcgatctcgc ctgctttttc gggctgctcg tcgtttacgt cattgcacac ttagcgatca
     2221 tacggccgta aaaatttttt ctcctccgtc acccgcacgc gggaattcgc tttcttcgcg
     2281 gttgcgcccg gaaacgtaaa cggaacttct ctccatccct cgcgatgatc ctcgaccatc
     2341 ttcacaaaga ccagctcagc cgctaccccg gcatagacat tttccgcggg ttcgccatct
     2401 tcaccatgct ctgcgcgaac tcggctgcgg aaagtctcgc ggcgccgcac agctttctcg
     2461 tccgcatcta cggctctttt gcagcgccgg ttttcgtttt tctcgcagga ttcatggtgg
     2521 cgctcggcgg caggaaacat cccgcttcgt atttcgtccg gcgcgggctc gagatcatcc
     2581 ttaccggtgc gctcatcgat ctcttcatct ggcgcattct tccgttcacg acgttcgacg
     2641 tgctctacct caccgggttc ggcatttgtc tcggcggatt ttttacaaag ctggaattac
     2701 cggcgaaaat cgcgctgacg gtgctgcttc ttgcagcggg tcccgtgctg cagctcctct
     2761 tttattacga gcccggcgtg cacgagttcg atctcacgca gggtttttcc tcactcagct
     2821 ttccggccgg ctggcactgg tggatcaaaa gctggttgtt cgacgggtgg ttccccgttt
     2881 tcccctggct cgggttgttc ttcgcgggat cgctcgtggc aacgaacgga actgttattc
     2941 tccggaagcg tgcgctgttt tttatcctcg ggtttcttct tttctgcggc gggatcgcgt
     3001 ggctttattt ccaccgcccc atcgaagaac gcgaaggata cagcgagctt ttttatccgc
     3061 cgggacttgc ctatctcgct gcggcctgcg gcgtcattct ctgcggactt tcgctgcttc
     3121 cttacctgaa ctcacacccg ttcttccgcc cgctgcgctg gctcggttcg tgcagtcttt
     3181 tcatttacat cctgcattcc gcgctcattt attttgtgct cgataattac ttcacggaac
     3241 tttcgctgaa aaatttcctg ctgatgtatc tcgttttcgc cgtggtgaac atcgcctgcg
     3301 cgggcatcct gttccagctg aaacaacagg acggctggaa aaaagttccg aaagtgcttc
     3361 gttttatttt cggcggataa ggttttgatc tccggcccgg cgcttgtcat ccgcaaaacg
     3421 gattcccgct tcaccgcgtc ccgttgaaat acagcatttc ccaacggccg gccgtcacgt
     3481 tgtacacgac gctgaaataa cacggaccgc catcgtacac tttcaggatg tacgtattcc
     3541 acgtaatatc atttacatcg cgcagatcgc atgtcgcatt gaataaaaga agatgctcct
     3601 tgtttttatc ctgcatgccc agcacctgaa aatggtattg cccgaatggg cgcatgttcc
     3661 tgaaatcctc cgccatcacg aaattttttt cgttcacttt ctgcagcgaa tccagccctt
     3721 cccgcaggtg tgaaaatgcg aaagcgatta ccgacgaatc cgcatcccag caattgtatt
     3781 tcgaataccc gctcaaaaga aaattttcct tcacacccgg aagaacgacg taacgcgggg
     3841 catttcccgc cgtatcctgc tgcgcaaacg cgcggagcgt gaacaacagg cagcccgcca
     3901 tgaataaaaa cctgctcata tcgcgacgaa ttctattttg ctttttgcga gccgttcgta
     3961 aaaatcgcgt ttccgcttgg aaggttttcc gccgaagaaa atacggtcga ggccggtcca
     4021 cacgaagaac cagccggccg gctgcaggat gatcagcagg aagttgtgcc ataatttctg
     4081 cgactcgatc gcgttcacaa gaacggcaca ggccagcagc aaaaatcccg ccgcggtcat
     4141 ggctgcgcca cggccataat tcgctttcac ttccgccgaa aaaacggcga actgccgctt
     4201 gaagtcgaac gccaggcgtt ccatgatgat cttctccgat ttttcatccc ggtttttctg
     4261 cgggatctgg aagcggacga ccttcacaaa gtcatcgcgt tcattcgtca gcttgttgag
     4321 ctccccgaga aagtcgtctg aaataacgcg ttcgctgaaa gggcgcggat cgaaatccga
     4381 aaaaagatca tcgtacgtgt cgatccagat attcagatcg cgcgaataag catggtgctt
     4441 gtcgtgatgc gtcgacgcca ttgggtcctg ttttgtgttg cgcccggctt acgtagcgca
     4501 gacttcgcac gaagttccgg cattcatcac ctgcagcggc ttgcagccgt cacgaaaaac
     4561 ggtgatccct ttcagtccga ggtcgcgcgc cttcgaatag atctgcgcga tatcctcccg
     4621 cgcggcgctg ttcgggagat tgatggtttt cgaaacggcg ttgtcggtga attcctggaa
     4681 cgccgcctgg tgcgcgagat gccagtgtgg accgatgtgc tgggccgttt tcagcagcag
     4741 cttcaattcc gcgggaagat ccgtgttctc cacgtggcct tccccgcgca ttttcttcag
     4801 cacatgttca gaaagcagtt tcttttcccc gagcagcttc gtcacaacgc tgttctcttc
     4861 caccagtgtt ttgtgaccga gcacgttttc cctccggaac gccagcgcaa aaagcggctc
     4921 gatggaagga gacgtgcctg caataacgcc gatcgtgccg gtcggtgcga tcgccgtgcg
     4981 cgtcgcgttg cgcaaaggca ccgcgggcgc gtaaacgctg tcgatccatt ccggaaatgt
     5041 gccgcgctcc gcgcataacg cgtgcgacgc ttccttcgcc acttcattga tgaatttcat
     5101 cagctttttc cccagcgcga tcgcttcctc cgaagcgtac ggaatgccga gcaggatgag
     5161 catttccgcc cagcccataa caccgagacc gatcttgcgg ttgccgcgaa ccgttttttc
     5221 gatctgcggc agaatgtact ggttctgctc aacgacatta tcaagaaaac gcaccgccag
     5281 catcaccgtc tcaccgagcg catcgatatc gatcgccggt tttccttccc gtgaaataac
     5341 gaaaagcgaa aggttcaccg aaccgaggtt gcaggcttcg aacggcagca gcggaacttc
     5401 cccgcacggg ttcgtgcagt tgatcacgcc ctgtgccggc gtcgggttcg ctttttcaag
     5461 cgcgtcgaga aaaacaagac ccggatcacc ggtccgccat gcatgatcga tgatggaatc
     5521 ccacagctgc acagccggaa tttcacgaac ggttttccgt gtgttcggat gaatgagcgg
     5581 ccatgccgcc ttctttttca cagcgtcgag gaatgcatcg tatacgccca ctgaaatatt
     5641 gaagttgcgc aggcggttca catccgtttt cgccgagatg aactcttcga cgtcgggatg
     5701 atcacaattg agaataccca tgttcgcgcc gcggcgtttt ccgccctgct tgatccgttc
     5761 cgtggacgcg tcgaacagct ccatgaacga aaccggaccg gaagccgttc ccccggacgc
     5821 aggaatgaaa tcgttcttcg gcctgatctt cgaaaagttg aaacccgttc ctcccccgct
     5881 ctggtggatg agcgccgctt ccttcagcgt atcgaaaatt ccttcgagcg aatcttccac
     5941 cggcagcaca aagcatgcgc tcagctgctg cttttcgagt cctgcgttca tcagcgtagg
     6001 tgaattcggg agaaagcgca gcgacgccat cagttcatag aacgcttccg catatttcaa
     6061 cggatcgccg ccgaatttct tttcacccat cgcgatggca ttggccacac gacggaacaa
     6121 ctgctccggc gtttcgtgca gcacaccgtc cgcatcacgc agcaggtagc ggctttcaag
     6181 aacggtaatg gcgttcggcg tcaggttcat ctttcaaagt tacggcagga atgacgaaac
     6241 aatgcaaacg ccgggcctgc acaatgaaac ccgggcattt cccgcttccg caacggtgta
     6301 attcgtactt tcgtccttgt cccattttaa cccgttttat gcctccgaaa gatctcaaag
     6361 ttgccgcgat acaggccatg cttcactggg aagaccgtga tgcgaacctg aaaatgttcg
     6421 atcgcctgct cgacacgatc actgaaagcg tcgaccttat cgtgcttccg gaaatgttca
     6481 ccacgggttt ttccatgtcg ccggaaaagt tgggcaagaa caccgacggt gaagcgcttt
     6541 cctgg
//
LOCUS       DASTSF010000197         5234 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_21991,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000197 DASTSF010000000
VERSION     DASTSF010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5234
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21991"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(238..2943)
                     /locus_tag="VFU15_05660"
     CDS             complement(238..2943)
                     /locus_tag="VFU15_05660"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdrD B-like domain-containing protein"
                     /protein_id="HEU4717294.1"
                     /translation="MKKILPVTFLLFLFSVTVIHAQIRRGDHFFRSGDYARAIPAYER
                     GLRHSQDAAAMENLANCYRITKNYTKAEEWYSKTVAANPGCNPMVYFYYGMVLRNNGK
                     NDEARAQFQKYMQKNPGDKKVTAQVQALDEMQVWMSQTPIYAVKPLTGINSPESDISP
                     VMFDNGLLFVSDRGEVDILNGENAPATGRAFYAVYYSKINYQNEDSVQFGKTKKLPRT
                     INKDYHNGPASVTEDGKLMAFNRVDDRIHLRSKHFVNRPKIYFSERKGKFWSNAKPFQ
                     YNSDAYSCAHPALSPDGQLLFFASDMPGSLGGTDIWMCKRDGDGWSKPENLGPDVNTP
                     GDEVFPEYRKDGMLFFSSDGLPGLGGLDIFSATKMSDGKWSDVTNQGAPLNSSFDDFA
                     VTFNSEGSRGFFTSNRPGGMGDDDVYSFKVTSKFLRISGKLLASKNTTDILPNTQVQL
                     MTKDGKLLKTTTTDSNGQFHFDNLSSDGSYIVRMDDQDPGISAKSKYYMADDKNDLVR
                     VTVMDEAGGKYTFQNLPADPSAPPQLLSDDDYLTIAGNLISDGQPPQPIANTAVKLTD
                     DQGNVVQTATTNDFGAFTFSHIPPDKSYIVSLADNSDPKLAASSRISITNKSGQELMS
                     TKPDANGKFQFKILKEDKSTLSAMTVTDADLRLDMKGTLVGADTMRTPLANTTISIMD
                     DKGKVVQSTTTDDHGNFSFVNLPADQSYIVSVDDVKDPGLVSFGKLYIKDENGKVVKT
                     LRLSAGGKFEFRILPLDRVTLGEVYVDDPWLQVLQMKAKAQSDSLLIIENIYYDYNDW
                     HILPAAEITLEKVVKVMQADPSITIVISSHTDSRGTAKANLELSQKRAQAVVDYLVKR
                     GIDKKRLTAIGYGETRLLNRCADGVDCSEEEHAKNRRTEFQINKK"
     gene            complement(2972..3913)
                     /locus_tag="VFU15_05665"
     CDS             complement(2972..3913)
                     /locus_tag="VFU15_05665"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HEU4717295.1"
                     /translation="MKKHLYIILSAAGIFLSGRIAAQQDPLVSQYMFNPLYINPAYAG
                     SRGVMNGDILLRKQWVGFPGAPTTEVFAVNAPTRKGKVGLGLEVIGDQIGPKRNSGLY
                     FDYAYRLPLGKGKLAFGLGAGIMNYRVNWNEIEYKDAQDPYASLGATNITKPDFKGGI
                     YFNNKRFYIGASITHMNQEAYVLIKDSLTLKANMSQHTFFTMGRAFQLGDNITFCPSI
                     LVRYTARNGPGSNTSTGDLNLNFRYKELMWAGISFRGDKELVFMAQYNLNSKWKLGYS
                     YDMSLGPLKTYESGSHEIVLAFDLNVFQSQILSPRFF"
     gene            complement(3929..4693)
                     /locus_tag="VFU15_05670"
     CDS             complement(3929..4693)
                     /locus_tag="VFU15_05670"
                     /inference="COORDINATES: protein motif:HMM:NF024974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HEU4717296.1"
                     /translation="MKRKHFRQRIFSFSGIVLTVFFGAVAAGAQTIGSISPVLVGSTG
                     NFSSNGGLMLSSSTGEVIVPTTANGAYILTQGFQQPSSVNALVLNAFIDTISSSCAGA
                     NDGEAIVHPAGGTGPYAYAWSTNDTTASIDSLSPGTYSVTVSDAGGLTTTQTFTITEN
                     NGLCGVHVYHGLTPNADGHNDIWIIDHLDLYLPNHVTVYNRWGMLVWQGDNYDNQKVF
                     WDGKAKDGSPLTDGTYFYVIEVDGYSPMNGWVELSH"
     gene            complement(4695..>5234)
                     /locus_tag="VFU15_05675"
     CDS             complement(4695..>5234)
                     /locus_tag="VFU15_05675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717297.1"
                     /translation="PAEQLDIASGNVRINGTNDYKYSTAKTHYYSVPAADFEPEAGAV
                     YARSMISGNAYLGSGTSTAVGYMDAPVHLPDGATVTSVTFYVVDNDGTYNLQPGQLWR
                     NDGSTSTSYGNIVQMANVPAPASSNSTLVQTSTTSAITSPVIDNQNYTYYLRWGTQQN
                     NANMRIVKVLITYTVTKAD"
BASE COUNT         1145 a         1357 c         1422 g         1310 t
ORIGIN      
        1 cgcgtaccag tcgccgccgc cgttgtactt cagcagcgca agctggcagc tgtatgtgtt
       61 ggtgacggaa aaggaagtga gcagtaaaaa cgaggcgagc gcgcaaaaga tgaccctgaa
      121 tttcatagtt ataaagatag cgaaacgata ctgtacggag tttgaaagtt cctggtttcc
      181 ggtttcaggt tccgggtcac agacgacaaa ccacagaaca aacaggaaac cgaaaatcta
      241 tttcttgttg atctggaatt ccgtccgccg gttctttgcg tgctcttctt cgctgcaatc
      301 gacgccgtcc gcgcagcggt tgagcaggcg cgtttcaccg tagccgatag ccgtgagacg
      361 tttcttgtcg atgccgcgct tgacgagata atccaccaca gcctgggcgc gtttctggga
      421 aagctcgagg ttggccttgg ccgtaccgcg tgaatccgtg tgcgagctga tgacgatcgt
      481 aatgctcgga tcagcctgca ttactttcac cactttctcg agcgttattt cagctgcagg
      541 aagaatgtgc cagtcgttgt agtcgtaata aatattctcg ataatgagca ggctgtcgga
      601 ttgtgctttc gctttcatct gcaacacctg cagccaggga tcgtcaacat acacttcgcc
      661 gagcgtcacg cggtcgagcg gaagaatgcg aaattcgaat ttcccgcctg cagaaagacg
      721 gagtgtcttc accactttcc cgttctcgtc cttgatgtac agtttcccga agctcacgag
      781 cccgggatct ttcacatcgt ccacgctcac gatgtacgac tggtcggccg gaagattgac
      841 aaagctgaaa ttaccgtgat cgtccgtcgt ggtcgattga accactttcc ctttgtcgtc
      901 catgatgctg atggtggtgt tcgccagcgg tgtgcgcatc gtgtcggcac cgacaagcgt
      961 tcctttcatg tcgaggcgga gatcggcatc cgtcactgtc atcgcgctga gcgtcgactt
     1021 gtcttctttc agaattttga actggaattt tccattggcg tcgggtttcg tcgacatcag
     1081 ctcctgcccg cttttgttcg tgatgctgat cctggaagaa gcagcgagct tcggatcgga
     1141 attgtcggcg agcgaaacga tgtagctttt atcgggagga atgtgggaaa aagtgaatgc
     1201 accgaaatcg ttcgtcgtcg ccgtctgcac aacatttccc tggtcgtccg taagcttgac
     1261 cgccgtgttg gcgatcggct gcggcggttg tccgtcggaa ataagattcc ctgcgatcgt
     1321 gaggtaatca tcgtcggaaa gcagctgcgg cggtgcggac ggatccgccg gcaggttctg
     1381 gaacgtgtat tttccgcctg cttcatccat caccgtcacg cgaacgagat cgttcttgtc
     1441 gtcggccatg taatatttgc tttttgcgga aatcccggga tcctgatcat ccatgcgaac
     1501 gatgtaggag ccgtccgaag aaagattgtc gaagtggaat tgcccgttgg aatcggtcgt
     1561 cgttgtcttc aggagcttcc cgtctttcgt catgagctgc acctgcgtgt tgggaagaat
     1621 atcggtggtg ttcttgctcg ccagtaattt tccgctgatg cgcaggaatt tgctggtgac
     1681 tttgaagctg tacacatcgt cgtctcccat tccgccgggc ctgttcgatg tgaaaaaacc
     1741 gcggcttcct tcgctgttga acgtcactgc gaaatcatcg aaggagctgt tcaggggcgc
     1801 gccctggttg gtgacatcgc tccattttcc gtcgctcatt ttcgtagcgg aaaaaatatc
     1861 gagtccgccg agaccgggca gaccatccga gctgaagaaa agcatgccgt ccttgcggta
     1921 ttcgggaaaa acttcgtctc cgggcgtgtt cacatccgga ccaaggtttt caggtttgct
     1981 ccatccgtcc ccgtcacgtt tgcacatcca gatatccgtt ccgccgagcg agccgggcat
     2041 atcggaagcg aagaaaagca gctggccatc gggagaaagt gcaggatgcg cacaggaata
     2101 ggcgtcgctg ttgtactgga aaggtttcgc gttgctccag aattttcctt tccgttcgga
     2161 aaaataaatt ttcggccggt tgacaaaatg tttgctgcgc aggtgaatgc ggtcgtcgac
     2221 acggttgaac gccatcagct ttccgtcttc cgtcacagaa gcgggaccat tgtggtaatc
     2281 cttgttgatc gtgcgcggaa gcttttttgt tttgccgaac tgaacggaat cttcattctg
     2341 gtaattgatc ttcgaataat aaaccgcata gaatgcacgg ccggttgcag gtgcattttc
     2401 accgttcagg atgtcgactt ctcccctgtc ggaaacgaaa agcaatccgt tgtcgaacat
     2461 cacgggcgaa atatccgatt cgggcgaatt gatcccggtg agcggtttca ctgcatatat
     2521 cggcgtctgc gacatccaca cctgcatttc atccagcgcc tggacctgtg cagtaacttt
     2581 tttgtcgccg ggattcttct gcatgtattt ctggaactgc gcacgagctt catcgttctt
     2641 cccgttgttg cgcagcacca ttccgtaata gaaatacacc atcgggttgc agccgggatt
     2701 cgcggcaacg gttttggaat accattcttc cgccttcgtg taatttttgg tgatgcggta
     2761 acagttggcg agattttcca tcgcggcagc gtcctgcgaa tgacgaagac cacgttcata
     2821 cgccgggatg gcccgggcgt aatcaccgga ccggaaaaaa tgatctcccc gcctgatctg
     2881 cgcgtggatc accgtaacgc tgaacaggaa aaggagaaac gtaaccggga gaattttttt
     2941 catacgtatt atttgcacaa tgcctgcggt atcagaagaa acggggtgaa agaatctgcg
     3001 actggaaaac attcagatca aaagcaagaa cgatctcgtg cgatcctgat tcgtatgttt
     3061 tcagcgggcc gagcgacatg tcgtaggaat aaccgagctt ccatttgctg ttgaggttat
     3121 actgcgccat gaagaccagc tccttatcgc cgcggaaaga aattccggcc cacatcaatt
     3181 ccttatagcg gaaattaaga ttgaggtcgc ccgtgctggt gttgctgccc ggaccgttgc
     3241 gggcggtgta gcgcaccagg atggaaggac agaacgtgat gttgtcgccg agctggaaag
     3301 ctcttcccat tgtgaagaaa gtatgctggc tcatgttcgc tttcagtgtc agcgaatctt
     3361 tgatcagcac gtaggcttcc tggttcatgt gtgtaatgga agcgccgatg taaaaacgtt
     3421 tgttgttgaa ataaatcccg cctttgaaat cgggcttggt gatgttcgtg gcgccgagcg
     3481 atgcgtacgg atcctgcgca tccttgtatt cgatctcgtt ccagttcaca cggtaattca
     3541 tgatgccggc gccgagcccg aaagcgagct ttccttttcc cagcggaagc cggtacgcat
     3601 aatcgaaata aagacccgag ttccgtttcg gcccgatctg gtcgccgatc acttcaaggc
     3661 cgagaccgac ttttcctttt ctcgtcggcg cattcaccgc gaacacttcc gtcgtcggtg
     3721 cgccgggaaa tcccacccat tgcttgcgca aaaggatatc gccgttcatc acgccgcggc
     3781 ttcccgcata agcgggattg atgtacagcg ggttgaacat gtactggctg acgagcgggt
     3841 cctgctgcgc tgcaattctt cccgacagga aaattcctgc tgcggagaga atgatgtata
     3901 aatgtttttt catgtttctt cctgctggtt aatgtgaaag ctccacccat ccgttcatcg
     3961 gcgaatagcc gtccacctcg atgacgtaga aataagtccc gtccgtcagc ggtgagccgt
     4021 ccttcgcctt gccgtcccag aaaactttct ggttgtcgta gttatcaccc tgccaaacca
     4081 gcattcccca gcggttgtaa acggtgacat gattcggcag gtaaagatcg aggtgatcga
     4141 tgatccagat atcgttgtgc ccgtcggcgt tcggcgtcag gccgtgataa acatgcacgc
     4201 cgcagagccc gttgttctcc gttatcgtga acgtctgcgt ggtggtaagt ccgcctgcat
     4261 cggaaaccgt caccgaatac gttccgggcg aaaggctgtc gatggaagcg gtggtatcgt
     4321 tcgtcgacca ggcatacgcg taaggtccgg ttccgccggc aggatgcacg atcgcttcac
     4381 cgtcatttgc accggcacac gaagagctga tcgtgtcgat gaacgcgttc agcacaagtg
     4441 cattcacaga ggacggttgc tggaatccct gcgtgagaat gtacgcgccg ttggccgtgg
     4501 tgggaacaat cacttctccc gtggaagaag aaagcatgag tccgccgttc gaagaaaaat
     4561 tccccgttga tcccaccagc acgggcgaga tcgaacctat cgtctgcgca ccggctgcaa
     4621 ctgcgccgaa aaaaacggtc agtacgatcc cggaaaacga aaagatcctt tgccgaaaat
     4681 gttttctttt cattttaatc agctttagtg acagtataag tgatcagtac ttttacaata
     4741 cgcatattcg cattgttctg ctgcgtcccc cagcgcaggt aataggtata attctggttg
     4801 tcgatcaccg ggctggtgat ggcactggtt gtgctggtct gcaccaacgt gctgttcgaa
     4861 gaagcggggg caggaacgtt cgccatctgc acgatgtttc cgtaagacgt tgaagtggac
     4921 ccgtcattcc gccagagctg gccgggctga agattgtacg ttccgtcatt gtccacgaca
     4981 tagaatgtca cggaggtcac ggtggcgccg tccggaagat gaacaggcgc atccatatag
     5041 cccaccgccg tcgacgttcc cgacccgaga tacgcgttac cggaaatcat cgaacgtgca
     5101 taaacagcgc ctgcctccgg ttcaaaatcg gcagcaggca cactgtaata atgtgttttt
     5161 gccgtggaat acttgtagtc attcgtgccg ttgatacgca cgttaccgct ggcgatatcg
     5221 agctgctcag ccgg
//
LOCUS       DASTSF010000198        14001 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1549439,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000198 DASTSF010000000
VERSION     DASTSF010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 14001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14001
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1549439"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            132..1982
                     /locus_tag="VFU15_05680"
     CDS             132..1982
                     /locus_tag="VFU15_05680"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEU4717298.1"
                     /translation="MKKTDKSVVLLVDDKAANLFSLENLLVAADRTFLTAANGKDALK
                     LALNKEIDLIILDVQMPDMDGFEVAQILKSNKKTRDIPIIFASAERKEHRFMLKGYEE
                     GAIDYLFKPLDPGIAKAKVAVQLKIRSQKKELLEKNRSLMQSELLIENSADIIGIIDA
                     TTLKIEEVNHAFQFILGYTPKEAKESPLFFFLANEERAMIQELSRQDKERLSFETRIY
                     CKDRTKKWLQWNVVVKNGKWFFNARDITEIMKIDRIRNYLATVVKQSSDAIYIHDHEG
                     RIISWNEGAEKIYGYSENEALQMKIWNIIPAQMHHEIEETVGRILAGEKIESHEAKRI
                     TRNGKLVDVLFSASVITDGNSELRSIAITERDITQQKIDAEQISKLNKELEEKVLQLV
                     ATNNELESFSYSVSHDLRAPLRALSGYSQMIEEDYGSVLDEEGMRLLGNIQSNARRMG
                     ILTDELLAFSRLGRKEIQKTQLDMEKIVRNVVLDAERTSPHRAVISAGEIFPATGDHT
                     LIQQVWVNLISNAIKYSAKKENPRVEIGATRSDGFVTYFVKDNGAGFNMDYADKLFGV
                     FQRLHNPNEFEGTGIGLAIVHRVITKHGGRVWAEAKKDEGATFYFTLPEE"
     gene            complement(1958..2788)
                     /locus_tag="VFU15_05685"
     CDS             complement(1958..2788)
                     /locus_tag="VFU15_05685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717299.1"
                     /translation="MVRTRYIVFVLVVVLVILVNLVRHFRGDDSSPRTKEYVALSAEK
                     MSRGDSAFVLSFYDQLQRIYDTLNTTDLCGRGELVFSGKKDLPADFVPKLITFRLLDF
                     IFDSAAAVKQTKQERDDAYESAFGYSNTLEDINSTMGNGNFKYDVSHRVFNGGFLNDV
                     KYIRKEKYIMVLLTCDLGLPRFEEGTSHFEPGRFSGGVAIFDIATTKLVGYYNCSAGS
                     DDEISALPRSKSGTDEMLLENLLENIRKSVEDVSQRAYGKPLSGMHGTGNAYSSGSVK
                     "
     gene            complement(2825..3832)
                     /locus_tag="VFU15_05690"
     CDS             complement(2825..3832)
                     /locus_tag="VFU15_05690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717300.1"
                     /translation="MIYIVWRRSGILVLVFLGLSFWILGQFYKDQTAHNHSYMAWSFF
                     SAAVATLLQSVLLFVMAKGEQTGSASAEQPPPSTWSSVRGHSFFAIPVVFWPLILGAA
                     SAWFFTSGSGTSSPAGTDAAAPAETEPRMINLLNPSGDTLFCTIIGTDGVVLTKTNYP
                     NSYARKKIDADVYEIRVTNAKGEVVLHIPSADATADGKKFSVRKDSAGKPELFRIIDA
                     PTDDADDYDDAWINVDGKHDMVLVDITSICNDSVTEKEIEKTDWTQFIRETYSGTDLI
                     EPRCPDGTGTILDTGADIPVTVGKSGHVFALFAVPSDKPVTAGMLADKIVVRCPRFSG
                     E"
     gene            complement(4046..5971)
                     /locus_tag="VFU15_05695"
     CDS             complement(4046..5971)
                     /locus_tag="VFU15_05695"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEU4717301.1"
                     /translation="MKKKFIQCSLLCGMIISSALHAQILHPPRIIPCSWSEDYSSATG
                     WTQVGTTVSVSGGAATFANTPDGNNTSRRIYKSIGSVISSSDTWKTDFDFMPSAVGPS
                     GGPGHILLAFTAGNLDPVCYSNYTYTNQDVIMATWESVNANPNSPYYLYGMARNDNGG
                     IYSSQPAIVLTTGTQYYLRLERINATEGQISAYTDAARTIPVAGSPQCFTIPSGVTGL
                     SYIQHGNITNGAPTRAMTSTVDNVCIRVGDPDGGTIAYNGYICGSGAPTIQSTQSPAG
                     GFWQWTYGWQMSAGCTGTWTTVPGATSATLSGVNISQNTCFRRIAYGPCGAFWYSNDV
                     TAIVYQVPTPVVSGSGFDACDNDWTLSASVPPAQAQGISYTWSTGQTGTPIDVTVTNP
                     NTIWVTASNACYSGQAGYWTSPCIEPRDTFSELAVTPFFCVDWSGNNPQEFFSIRDLN
                     LPPGAGPAYNATDGTLQIFPRWNSSNPCIYTQTAHSTCNGFWNGVFVWSGIYNGSPVP
                     GGVYDWVFTLTNCNRQQVFNGTVYVNGTCRFANPDPHPQDTAAAGNGADPASQLNVFP
                     NPGSGKYTVALPALNTTETARTLSVTDAYGRVIRNIDISSSDQQAEIDLSNEADGVYF
                     FMITDAASSRVVRVIKD"
     gene            complement(6082..6807)
                     /locus_tag="VFU15_05700"
     CDS             complement(6082..6807)
                     /locus_tag="VFU15_05700"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEU4717302.1"
                     /translation="MKPVRVLIVDDEADAVAGLRTMLRDFCTGVEVVGESSSAIEGVK
                     LIRRLQPDAVFLDVEMPGGDGFSLIDSLDKANRPAIIFTTAHREYAISALREQAFDYL
                     LKPVDIDELQNAVEKAKNFLASRNSRELQPGEEQRMRVSTPEGIVFIPLSEIIHIEAS
                     GRYSIVHSVHAGSHTVTRNIGEYEEELTGSGFFRVHKSHLVNCRHALRISSADGGFLE
                     LSNGRSIEISRRKKSEFMLRMKG"
     gene            complement(6804..9755)
                     /locus_tag="VFU15_05705"
     CDS             complement(6804..9755)
                     /locus_tag="VFU15_05705"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEU4717303.1"
                     /translation="MRFLFLFLLAGVALPAQQLSSIHYDVESGLPSSELYDVHQDRQG
                     FIWLASDRGVSRFDGYTFRNFTKEDSLGDNTIFNIYEDGTGRLWFNAFNGALSWYGNG
                     HFHLYAFNDSIRAYTRPPRILRSFACTGNSLVLGYYDGGMLSIDSTGHVSAMQEYPGP
                     PSGFFAVSGKRWPAFAVGRSLCRPDSVTNRQVRGFSYSINGHEGIVNIPLLKPTEKEL
                     ILALPRRNGSFSFGLVHTFVEISGDGIVHTETFDDEIIRICEDNDSCLWVSFRKGGVK
                     RYTRNGIPGTAACETFFGSEIVSCVFQDSEGGFWITTLGNGVYYVPCISFRTYFTSGD
                     RNTTAVTAIVPDGKGGVFAGMRNGIVHHYTTGGNWETYNAAAPGFSGDQALAGLFCDT
                     LKQQLYVAFSLGGGLRRMDRSGNFSGLSPTNLHCVTTGAGGNIYTGSNNEIGCMNDSD
                     RSITLKRGISIDAVYTDREGTTWMGGMNGLYRMRGDSAVAMDPRSPVMHTRITGIAGM
                     PDGRLALATVGSGVLFFDGKNTTSVSEKDGLCGDIVNGVATGGNGELWVATNKGVSVL
                     YDSAGAMRIKNFHREQGLPTEEVMCIAVSGGNVWLGTRFGLTSLPEKETERNLTPPPV
                     YLYSVMAGDSSLSLGDDAEADLGYARNSFRFSFAGLSFRAQGKVHYRYHLEGLNDDWV
                     ETDAPQAIFFSLDPGEYRFTVYALNNDGIPSVFPATFSFRIRPPFWRTWWFILVAAAG
                     VVSVAVYLILLSFSRAREKHEMRARISEYQQQALANQMNPHFIFNSLNSIQSYIAQED
                     RRSAIRYLSRFSRLMRLSLDHSREKTVPLKEELELVSVYMELEAMRFKERMEWSMETE
                     EGLDYSRYEIPAMLIQPYVENAIRHGLLNRDSAGGKVTVRIYTIGERLCCEVEDNGIG
                     REAAGKIGERNRHRSAGTSITSSRLRLLCRTMDCPFHFEIIDRNNDRTQHSGTIVKFD
                     LPYRKKTANRK"
     gene            complement(9877..10767)
                     /locus_tag="VFU15_05710"
     CDS             complement(9877..10767)
                     /locus_tag="VFU15_05710"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEU4717304.1"
                     /translation="MKKTLPVYSLEQFLAPGPDNGIYVNTIPRHLAKYEFLFGPHRHD
                     SFLVILCTKGKGRHEIDFNAYDIRPGSLFFIYPGQAHLFTLTEPADGYVFLHSKDFYE
                     TYFTRKKISAYPFFYCSHNTPALYLDPAQVRYAEELMKEMLSEFEGKALAKNQKICSL
                     IDIAYIEMARIYLEKNPVTLPGSQHSMRIRQLEDLIEKNFRTERSPSFYASEMNMSAK
                     QLNRICRETLGKTTSEMISDRVVLEAKRMLAHTDAPFAQIASELGYFDDAYFIRMFRK
                     HSGKTPAAFAKKYREESIVQ"
     gene            10869..11216
                     /locus_tag="VFU15_05715"
     CDS             10869..11216
                     /locus_tag="VFU15_05715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717305.1"
                     /translation="MRLPSFQKVSCRLNFAFPQKMDNTTKKQPPGMCEAIIEEMQFFL
                     SHKVRKPITSAHGLALILKQNKIKDCEKETALCYLIDSIHEMQEATREMNLLLEKAKE
                     RVRGAGEPDHPGE"
     gene            11333..>14001
                     /locus_tag="VFU15_05720"
     CDS             11333..>14001
                     /locus_tag="VFU15_05720"
                     /inference="COORDINATES: protein motif:HMM:NF012879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEU4717306.1"
                     /translation="MIKKVFFGAAAVAALLIFSAGSGDKEYKYETVPNDPLHGRIYHL
                     DNGLTVFLTVNKAQPRIQTMIATKAGSKYDPHDATGLAHYLEHMLFKGTDKYGTKDYA
                     KEKPLLDSIENLFEVYRKTTDTTERKKIYHHIDTLSYQASGYAIANEYDKMMTSIGAK
                     GTNAFTSVEETVFIEDIPSNEMEQYLQIESERFRNPVMRLFHTELEAVYEEKNRGLDN
                     DYEKTDEALMSGLFRKHPYGTQTTIGTIHDLKNPSLKNIIEYYHTYYVPDNMVIAMSG
                     DLDPDATIKLIDKYFGGFTSKPVPAYTPPQEDPIKSPIEKNVNGPEAANVTIGYRFAG
                     AGSDDADMISLISQMLSNYQAGLFDIDLTQQQKVLNAAAYSNVMKDYSVMEMQAYPVE
                     GQTLKDAQAKMQGELDKLKTGDFPDWLISAVVNNMRLQETQQYHDNFPRAYAFTQAFV
                     TGESWEHAVGQTDRLSKITKQQVMDFAKKNFTDDYVVVYKNEGTDTTVEKVTKPKITP
                     VQTNAEDESPFVKNIEAEPVTPLTPVYLDFSKDVKKYSVKNEMPVYYAQNNEDSTFSM
                     YILFDMSAKRNKMLKPALDYLQFAGTSTMSAAQVQQEFYKLACTYFTYAGTDQTYVGL
                     SGLTKNFVPAMKLLESLLSDVQPDEDALKNTVSSTMKDRENEELDKNTILFSAMYDYG
                     IYGKHSPFCNVLTNKELKNVKAKELTDLIHDLCNYPHHVLYYGSEPGQALVNDLGTLH
                     KMPAQFKPLPQADTFVQLKGTNQVYIIDHDMKQAEIIILQQGGYYDPAIKPQAALYNE
                     YFGGSMASIVFQTLRESKALAYSTFCSYYAPSDTTEHYYNLAYIGSQVDKLPEAVDGM
                     MDLLNNDMPQYPQLWSSSRDAILKSIASTRIIR"
BASE COUNT         3365 a         3877 c         3812 g         2947 t
ORIGIN      
        1 accattgcac aggatccgca ggaagcgctg ttccccgtca tgccgctcgc cgcgatccat
       61 acgaaaaatg tccggcaagt gctcacactg gaaaacatcc ggaatttcct gctcactaaa
      121 atttctgcgc gatgaaaaaa acggacaaat ccgtggtcct gctggtcgat gacaaggccg
      181 cgaatctttt ttcgctggaa aacctgctcg ttgcggccga ccggacattc cttacggcgg
      241 ccaatggaaa agacgcgctc aagctggcgc tgaacaagga gatcgacctg atcatcctcg
      301 atgtgcagat gccggacatg gacggcttcg aggtggcgca gatcctgaaa tcaaataaaa
      361 agaccaggga tattcccatc attttcgcat ctgccgaacg gaaagaacac cgtttcatgc
      421 tgaaaggtta tgaagaaggc gccatcgatt acctcttcaa gccgctcgat cccggcatag
      481 cgaaagcgaa agtggcggtg cagctgaaaa tacggtcgca gaaaaaagag ctgctggaaa
      541 agaaccgttc gctcatgcaa agcgagctgc tcatcgaaaa ttccgccgac atcatcggca
      601 tcatcgacgc gacgacgctg aagatcgaag aagtgaacca cgcgttccag ttcattctcg
      661 gctatacgcc gaaagaagcg aaagaaagtc cgctgttctt tttcctggcc aacgaagaac
      721 gggcgatgat ccaggagctg agccggcagg acaaggagcg gctttcgttc gagacacgga
      781 tctattgcaa ggaccgcacg aaaaaatggc tgcagtggaa cgtggtcgtc aagaacggga
      841 aatggttctt caacgcgcgc gacatcacgg agatcatgaa gatcgaccgc atccggaatt
      901 acctggctac cgtcgtgaag cagtcgagcg acgccatcta catccacgat cacgaaggga
      961 ggatcatttc ctggaacgaa ggcgcggaaa aaatatacgg gtattcggaa aacgaagcgc
     1021 tgcagatgaa gatctggaat attattcccg cgcagatgca ccacgagatc gaagaaaccg
     1081 tcggccgcat cctggccggg gaaaagatcg aatcgcacga agcgaaacgg atcacacgga
     1141 acggaaagct cgtggacgtg ctcttttccg catccgtcat caccgacggc aacagcgagc
     1201 tccggtcgat cgccatcacc gaacgcgaca tcacgcagca gaagatcgac gcggaacaga
     1261 tcagcaagct gaacaaagag ctggaagaaa aagtgctcca gctcgtggcg acgaacaacg
     1321 agctcgaatc tttttcctat tccgtttcac acgacctgcg cgcaccgctg cgtgcgctga
     1381 gcggctattc gcagatgatc gaagaggatt acggctccgt tctcgatgaa gaaggaatgc
     1441 ggctgctcgg gaacatccag tcgaacgctc gccgcatggg aattctcacc gacgagctgc
     1501 tcgctttctc ccgcctcgga agaaaagaga tccagaaaac gcagctcgac atggaaaaaa
     1561 tagtgcggaa cgtcgtgctc gatgcagaac gcacctcacc ccaccgtgcg gtaatatcgg
     1621 ccggggaaat ttttcccgcc acgggcgatc acacgctcat ccagcaggtg tgggtgaacc
     1681 ttatctcgaa cgcgatcaag tattccgcga aaaaagaaaa tccacgggtg gagatcgggg
     1741 ccacgcgatc ggacggcttc gtcacttatt tcgtaaaaga caacggcgcg ggcttcaaca
     1801 tggattacgc ggacaagctc ttcggcgtgt tccagcgttt gcacaacccc aatgagttcg
     1861 aaggaacggg catcggtctc gccatcgtgc atcgcgtgat cacgaaacac ggcggcaggg
     1921 tgtgggcgga agcgaagaaa gacgaaggcg ccacgtttta ttttacgctg ccggaagaat
     1981 aagcgtttcc cgttccgtgc attccggaaa gcggtttccc gtaagcacgc tgcgaaacat
     2041 cctccacgct tttccggata ttctccagta aattttccag cagcatttcg tcggtgccgc
     2101 ttttcgaacg gggaagcgcg ctgatctcgt cgtccgaacc cgcggaacag ttgtagtagc
     2161 ccaccagctt tgtcgttgca atgtcgaaga tggcgacgcc cccgctgaag cgccccggtt
     2221 cgaaatgaga agttccttct tcaaaccggg gcagtccgag gtcgcaggtg agcagcacca
     2281 ttatgtattt ttctttccgg atatatttca cgtcgttcag gaaaccgccg ttgaaaacac
     2341 ggtgtgaaac gtcgtatttg aaatttccgt tccccatcgt cgagttgata tcctcgaggg
     2401 tattgctgta accgaatgca gattcatagg catcatcccg ttcctgcttt gtctgcttta
     2461 ccgctgccgc gctgtcgaaa ataaaatcca gcaggcggaa cgtgatcagc ttcgggacaa
     2521 aatccgcggg aagatctttc ttccccgaaa acaccagttc ccccctcccg cacaggtccg
     2581 tggtgttcag tgtatcatag atacgctgca gttggtcgta gaacgacagg acgaatgcag
     2641 aatccccgcg gctcatcttt tccgcgctga gcgcaacgta ttctttcgtt cgcggtgaag
     2701 aatcatcacc ccggaaatgg cggacaagat tgacaaggat gacaaggaca accaccagca
     2761 cgaaaacgat gtagcgcgta cggaccattt gtttcagctc tgagctgcgg agaatgccag
     2821 cgtcctattc accggaaaaa cgcgggcacc tcaccacgat tttatccgcc agcattccgg
     2881 cggttaccgg cttatccgaa ggaacagcga acaatgcgaa aacatgtccg ctctttccaa
     2941 ccgtcaccgg aatatccgca ccggtgtcga ggatggtgcc ggttccgtcc ggacagcggg
     3001 gttcgataag atcggtcccg ctgtatgttt cgcggatgaa ctgcgtccag tcggtttttt
     3061 caatttcctt ttcggtaacg ctgtcattgc agatggacgt gatgtccacg agaaccatgt
     3121 cgtgtttccc gtcgacattg atccatgcat cgtcataatc atccgcatcg tccgtgggcg
     3181 cgtcaatgat gcggaacagc tccggtttgc ccgcactgtc tttccgcacg ctgaattttt
     3241 ttccgtcagc cgtcgcatct gcagaaggaa tgtgaagcac cacttctcct tttgcgttcg
     3301 ttaccctgat ctcgtataca tcagcatcga tctttttccg cgcgtagctg ttcgggtagt
     3361 ttgtctttgt caggaccacg ccatccgtac caatgatggt gcagaagagc gtatcccccg
     3421 aagggttgag caggttgatc atacgtggtt ctgtttcagc cggcgcagca gcatccgtac
     3481 cggctggtga agatgttccg cttcccgaag taaaaaacca ggcggaagct gcgccgagga
     3541 tcagcggcca gaaaacaacg ggaatagcaa agaagctgtg cccgcgaacc gacgaccagg
     3601 ttgagggtgg tggttgttct gcagaagcgc tgcctgtttg ttcgcctttg gccataacaa
     3661 aaagaagaac cgactgcagc aacgttgcca ctgccgcgct gaagaaagac catgccatgt
     3721 aggaatggtt gtgtgcggtc tggtctttat aaaattgtcc cagtatccag aagcttagtc
     3781 cgagaaagac caggacaaga attccggaac ggcgccatac gatgtagatc atggaatttt
     3841 tttgaggtcc gttttattcc agggaacagg tgaaaaaaaa tcgcccggtt ccgttgccgg
     3901 tgaaaatatt caatgcgatc attccaggta gaaattgtaa gcaccggaac ctgttctgta
     3961 gcgaccggtc cattcctgta acgaccggca aaaaaacgtc ccggcaaaaa gccgggacgc
     4021 gggggagtga agggttctgc tgtgtttaat cttttatgac gcggacaacg cgcgacgacg
     4081 ctgcgtcagt gatcatgaag aaatagacgc cgtccgcttc attgctaaga tctatttccg
     4141 cctgctgatc ggaagacgag atatcgatat tcctgatcac gcgcccataa gcatccgtta
     4201 cggaaagtgt gcgcgcggtt tctgtcgtgt tcagcgccgg cagcgcaacc gtatattttc
     4261 cgctgcccgg gttcggaaat acattgagct gcgaagcggg atcagcgccg ttacctgcgg
     4321 ccgctgtatc ctgcggatgc gggtccgggt tggcgaagcg gcacgttccg ttgacataaa
     4381 ccgttccgtt gaacacctgc tgcctgttgc agttggtaag cgtgaagacc cagtcatata
     4441 caccgccggg aacaggactt ccgttgtaaa ttccgctcca cacgaatacg ccgttccaga
     4501 aaccgttgca ggtggaatga gccgtctgtg tgtagatgca cggattgctg ctgttccacc
     4561 gcgggaagat ctgcagtgta ccgtccgtgg cattatacgc cgggcctgcg ccgggcggca
     4621 ggtttaaatc gcggatggaa aaaaattcct ggggattatt accgctccag tcgacgcaga
     4681 agaacggcgt gacggcaagc tccgaaaacg tatcccgtgg ttcgatgcac gggcttgtcc
     4741 agtaaccggc ctgcccggaa taacacgcat tgctcgccgt cacccagatg gtattgggat
     4801 tggtgaccgt cacgtcgatc ggcgttccgg tctgccccgt gctccacgtg tacgaaattc
     4861 cctgcgcctg cgctggcgga acagaagcgg acaatgtcca gtcgttatcg cacgcatcga
     4921 aaccacttcc ggatacgacg ggcgtgggca cctggtaaac aatagccgtc acatcattcg
     4981 aataccagaa agcgccgcag gggccgtagg cgattctcct gaagcaggtg ttctggctga
     5041 tattgacacc ggaaagcgtc gcactcgtgg cgcccggaac cgtggtccac gttcccgtgc
     5101 atcctgccga catctgccat ccgtaagtcc attgccagaa acctccggcg ggcgactgcg
     5161 tgctttggat cgtcggtgca ccggatccgc agatgtaccc gttgtatgcg atcgttccac
     5221 cgtccggatc tccgacacgg atgcacacgt tgtccacggt acttgtcatg gcgcgcgtgg
     5281 gtgcgccgtt ggtgatattg ccgtgctgga tataggaaag cccggtgaca ccggaaggga
     5341 tcgtgaagca ttggggagaa cctgcaacgg gtatcgtgcg cgccgcatcg gtgtaggcgc
     5401 tgatctgtcc ttccgtcgca ttgatgcgtt caaggcgcag gtaatattgc gtgccggttg
     5461 taagaacaat tgcaggctgg gaagaataga tacctccgtt gtcattcctt gccattccgt
     5521 agaggtaata cgggctgttg ggattcgcgt tgacggattc ccacgtggcc atgatcacgt
     5581 cctggttggt gtaggtataa ttgctgtaac aaacgggatc gaggttgccc gctgtaaatg
     5641 caagcaggat atgaccgggt ccgccgctgg gtccgaccgc cgacggcata aaatcgaaat
     5701 cggttttcca cgtgtcggac gaagagatca cgctgccgat gcttttgtaa attctccggg
     5761 aagtgttgtt gccgtccggt gtgttggcga atgtggcggc gccgccggaa acggaaacgg
     5821 ttgtgcccac ctgcgtccac ccggtggcgg atgaataatc ttccgaccag gaacagggaa
     5881 tgatcctcgg gggatgcagg atctgggcat gaagtgcgga agaaatgatc attccgcaca
     5941 gcagggaaca ttgaataaat ttctttttca taggaacagg attgttttcg gacaaaacta
     6001 ttcccgtgag aaagaaggca ggtctgattg taacgatcgc ggggaaattg taaaaaacgg
     6061 ttccgccggt gtaatgaacg gtcagccttt catgcgcagc atgaactcgg attttttccg
     6121 gcgcgagatc tcgatgctcc ttccgttgct caattcgagg aacccgccgt cggcggatga
     6181 gatgcgcaac gcatgacggc aattgacgag atgtgatttg tgaacgcgga aaaagccgga
     6241 accggtgagc tcctcttcgt attcgccgat gttgcgcgtc acggtatggc tgccggcatg
     6301 aaccgagtgg acgatggaat aacgtccgga agcttcgatg tgaatgatct cgctcagcgg
     6361 aataaaaaca attccctccg gggtgctgac gcgcatgcgc tgctcttcac ccggctgcag
     6421 ctcccggctg ttacgcgaag cgagaaaatt tttcgctttc tccacagcgt tctgcagctc
     6481 gtcgatatcg accggcttca gcaggtaatc gaacgcctgc tcgcgcaatg cggagatcgc
     6541 gtattcccgg tgcgcggtag tgaaaatgat cgcaggacgg ttcgctttgt cgagcgaatc
     6601 gatgagtgag aacccgtcgc caccgggcat ttccacatcg aggaaaacag cgtcgggctg
     6661 caggcggcgg atcagcttca cgccttcgat cgccgaagac gattctccga ccacttcaac
     6721 gcctgtacag aaatcgcgga gcatcgtgcg cagcccggca actgcgtcag cttcgtcatc
     6781 cacaataaga acacgcaccg gtttcatttc ctgttcgctg tttttttacg atacggaaga
     6841 tcaaatttaa caattgtgcc cgagtgctgc gtcctgtcat tattacgatc aataatttca
     6901 aaatggaacg gacaatccat cgtcctgcac agaagacgaa gacgcgatga cgtgatcgac
     6961 gttccggcgg accggtgcct gttccgttca ccgatctttc ccgccgcttc gcggccgatg
     7021 ccgttatcct ctacctcgca gcacagccgt tcaccgatcg tgtaaatgcg caccgtcact
     7081 ttcccgccgg cgctgtcgcg gttgagaaga ccgtgcctga tggcattctc cacgtaaggc
     7141 tggatgagca tcgccgggat ttcatagcgg gaataatcaa gaccttcttc tgtttccatc
     7201 gaccattcca tcctttcctt gaagcgcatc gcttccagct ccatatacac cgacaccagc
     7261 tccagctctt ctttcagcgg aacggttttc tcccgggaat gatcgaggct gagacgcatc
     7321 agcctggaaa aacgcgagag gtaacggata gcgctgcgcc ggtcttcctg cgcaatgtag
     7381 ctctgtatcg aattcagcga attgaaaatg aaatgcgggt tcatctggtt cgcgagcgcc
     7441 tgctgctggt attcggaaat gcgtgcgcgc atctcgtgct tttcccttgc gcgtgaaaaa
     7501 gaaagaagaa tgagatagac cgcaacggaa acgacgcccg cggcagcaac aagaatgaac
     7561 caccacgtac gccagaacgg cggacgtatc cggaaagaga acgtggccgg gaatacgctg
     7621 gggatcccgt cgttgttcag cgcatatacg gtgaacctgt attcgccggg atcaagcgaa
     7681 aagaaaatgg cctgcggcgc atccgtttcc acccagtcat cgttcaatcc ttcgaggtga
     7741 taccggtaat gcacttttcc ctgcgcacgg aaggaaagtc ccgcaaacga aaaacggaat
     7801 gaattccttg cataccctaa atcggcctcc gcatcatccc cgagcgaaag cgatgaatcc
     7861 ccggccatga cgctgtacag gtagaccggt ggtggcgtaa ggttacgctc cgtttctttt
     7921 tccggcaggc tggtcagccc gaacctggtg ccgagccaga cgtttccgcc cgacacggcg
     7981 atgcacatca cttcttccgt gggaagtccc tgctcgcggt gaaagttttt tatccgcatc
     8041 gcaccggcgc tgtcgtagag aacgctcacc cctttatttg tcgccaccca taattctccg
     8101 ttcccgcccg tcgccacacc gttgacgata tcgccgcaga gaccgtcctt ttcggaaaca
     8161 gaagtcgtgt tttttccatc gaagaaaagc accccgcttc ccacggtcgc caatgcaagt
     8221 cttccgtcgg gcatgcctgc aattcccgtg atccgcgtat gcatgacagg actgcgcgga
     8281 tccatcgcaa ccgcggaatc accccgcatg cggtacagcc cgttcattcc gcccatccat
     8341 gtcgttcctt cccggtcggt gtaaacagca tcgatgctga tgcctctctt cagcgtgatg
     8401 ctgcgatcgc tgtcgttcat gcagccgatc tcgttgttcg acccggtgta tatgttcccg
     8461 ccggcgcctg tcgtcacaca atgcaggttg gtgggcgaaa gcccggaaaa atttccgctc
     8521 ctgtccatcc gccgcagtcc gccgcccagc gaaaaagcga catacagctg ctgcttcagc
     8581 gtatcgcaga aaagtccggc gagcgcctgg tcgccgctga aacccggtgc ggccgcattg
     8641 tacgtctccc agtttccgcc cgtggtgtaa tgatggacga tgccgttgcg cattcctgcg
     8701 aacacgcccc ccttcccgtc cggcacgatc gccgtcacgg ctgttgtgtt gcggtcgccc
     8761 gaagtaaaat aggtacggaa gctgatgcat ggaacataat acacgccgtt tcccagcgtg
     8821 gtgatccaga atccgccttc gctgtcctgg aaaacgcacg aaacgatctc gctcccgaaa
     8881 aatgtttcgc aggccgctgt cccgggaatt ccgttgcgcg tgtagcgttt cactccgcct
     8941 ttgcggaacg aaacccagag acaggaatcg ttgtcttcgc agatgcggat gatctcgtca
     9001 tcgaacgttt cggtatgcac gatgccgtcg cccgaaattt ccacgaacgt gtgcaccagg
     9061 ccgaaggaaa agctgccgtt cctgcggggc agggcaagga tgagctcttt ctccgtcggc
     9121 ttcagcaaag gaatattcac gatgccttca tgaccgttga tgctgtagct gaatccgcgc
     9181 acctgccggt tcgtcacgga atccggccgg cacaagctgc gccctacggc gaatgccggc
     9241 catcttttcc cgctcacggc gaaaaaacca gacggcggtc ccgggtattc ctgcatggcg
     9301 gaaacgtgtc ctgtgctgtc gatggaaagc attcctccgt cgtaataacc gagcacgaga
     9361 ctgtttcccg tacacgcgaa cgaacgaaga atgcgcggcg ggcgggtata cgcgcggatg
     9421 gaatcgttga acgcatacag gtgaaaatgg ccgttgccgt accaggaaag cgctccgttg
     9481 aatgcgttga accagagacg acccgttcca tcttcataaa tgttgaagat cgtattgtcg
     9541 cccaggctgt cttctttcgt gaagttgcgg aacgtgtagc cgtcgaagcg actcacgccg
     9601 cgatccgatg caagccagat aaatccctgc cggtcctggt gcacatcgta cagctccgag
     9661 ctgggcaaac cgctttcgac atcgtaatga atggaagaaa gctgctgcgc gggcaaagcg
     9721 acgcctgcca gcaggaacag gaacaggaaa cgcatcgtga ccgaaaatag ccggaaatac
     9781 gcacataagc caggtgacgg aaaagctgca ccgtgcgaat tcagcggcag gttcccggcc
     9841 atggcgtgtg atcgcctccg cggctgcccg gcacgttcac tgcacaatac tttcctccct
     9901 gtattttttc gcgaaagccg ccggcgtttt cccgctgtgc ttccggaaca tgcggataaa
     9961 atacgcgtca tcgaaataac cgagctccga cgcaatctgc gcgaaaggcg catccgtatg
    10021 cgccagcatg cgttttgcct cgaggacaac ccggtcgctg atcatttccg atgtcgtttt
    10081 tcccagcgtt tccctgcaga tgcggttgag ctgcttggcg ctcatgttca tttccgatgc
    10141 gtagaacgac ggcgatcttt ccgtacggaa atttttttcg atcaggtctt ccagctggcg
    10201 gatgcgcatc gagtgctggc tgccgggcag cgtcaccggg ttcttttcga ggtaaatgcg
    10261 cgccatctcg atgtaagcga tgtcgatcag cgaacatatt ttctggttct tcgccagcgc
    10321 ttttccttcg aactcggaca gcatttcttt catcagttct tccgcatacc gcacctgcgc
    10381 gggatcgaga taaagcgcag gcgtgttgtg cgagcaatag aaaaacgggt atgcgctgat
    10441 cttcttccgc gtgaaatagg tttcgtaaaa atccttcgaa tgaagaaaga catagccgtc
    10501 cgcaggttcc gtgagcgtga acaggtgggc ctgtcccggg taaatgaaaa aaagacttcc
    10561 cggccggatg tcgtatgcat tgaagtcgat ctcgtggcgg ccttttcctt tcgtgcagag
    10621 gatgacaaga aaagaatcat gccggtgcgg accgaaaaga aattcgtatt tcgcgaggtg
    10681 ccgcgggatc gtgttcacgt aaattccgtt gtccggacca ggtgcgagga actgttccag
    10741 gctgtacacc gggagtgttt ttttcatggg gaagcagtta tgctgtgcga caaaagcggg
    10801 ggatgtgcaa tggaaatgta ggccaaattt ccgaacggct gttccctttg tcctttttgt
    10861 cctgcattat gcgtcttcct tcctttcaga aagtgtcctg ccggctgaat tttgctttcc
    10921 cgcagaaaat ggacaacacg acgaaaaaac agccgcccgg gatgtgcgaa gcgatcattg
    10981 aagaaatgca gttctttctc tcgcacaaag tgcgcaagcc catcacctcc gcgcacggac
    11041 tcgcgcttat cctgaagcag aacaaaataa aagactgcga aaaagaaacc gcgctctgct
    11101 acctcatcga ttccattcat gaaatgcagg aagcgacgcg cgagatgaac ctgctgctgg
    11161 aaaaggcgaa agagagagtc cgcggggcag gagaaccgga tcatcccggc gaatgaaaaa
    11221 cggcgcagga ccggtccggg aaaagtgaag cggaaaaaag tgttggttcc cgctcccaaa
    11281 tctcctaatt tggttcttcc aatttcaccg gaatcttctt aaaacccgta ccatgatcaa
    11341 aaaagtcttc ttcggcgctg ccgctgttgc cgccctgctc attttttctg ccggctccgg
    11401 cgacaaggaa tacaaatacg aaaccgtccc gaacgatccg ctgcacggcc ggatctatca
    11461 cctcgacaac ggcctcaccg tttttctgac ggtgaacaaa gcgcagccga gaatacagac
    11521 gatgatcgcc acgaaagcgg gaagcaagta cgacccgcac gatgcgacgg gtcttgccca
    11581 ttacctcgag cacatgctct tcaagggaac cgataaatac ggcacgaaag attacgcgaa
    11641 agagaaaccg ctgctcgaca gcatcgagaa tctcttcgag gtgtaccgca aaacgacgga
    11701 caccaccgag cgcaaaaaaa tatatcatca catcgacacg ctttcgtacc aggcttcggg
    11761 ctatgcgatc gcgaacgaat acgacaagat gatgacgtcg atcggcgcaa aaggcacgaa
    11821 tgctttcacg tcggtggaag aaaccgtttt catcgaggac attccttcca acgaaatgga
    11881 gcaatacctg cagatcgagt cggaacgttt ccgcaatccc gtgatgcgcc tgttccacac
    11941 cgagctcgag gccgtttatg aagagaagaa ccgcgggctc gacaatgatt acgaaaaaac
    12001 ggacgaagcg ctgatgagcg ggctgttccg gaaacacccg tacggcacgc agacgacgat
    12061 cggcacgatc cacgacctga agaatccttc gctgaaaaac atcatcgaat attatcacac
    12121 gtactatgtg ccggacaaca tggtgatcgc tatgtcgggc gacctcgacc cggacgcaac
    12181 gataaagctg atcgataaat atttcggcgg gttcacgtcg aaacccgttc ccgcgtatac
    12241 gcctccgcag gaagatccga tcaaaagtcc catcgagaaa aacgtgaacg gacccgaagc
    12301 ggcgaacgtc acgatcggtt accgttttgc cggagcagga agcgatgacg ccgacatgat
    12361 cagcctgatc tcgcagatgc tgagcaatta ccaggcagga cttttcgaca tcgatctcac
    12421 gcagcagcag aaagtgctga acgcagcggc ctacagcaac gtgatgaagg attattcggt
    12481 gatggaaatg caggcgtatc ccgtggaagg gcaaacgctg aaagacgcac aggcgaaaat
    12541 gcagggcgag ctcgacaagc tgaagaccgg cgacttcccc gactggctca tcagcgcggt
    12601 ggtgaacaac atgcgcctgc aggaaacaca gcagtaccac gacaacttcc cccgcgccta
    12661 cgcgttcacc caggcgttcg tcaccggtga aagctgggag cacgccgtcg ggcagaccga
    12721 ccgcctctcg aagatcacga aacagcaggt gatggatttc gcgaagaaaa atttcaccga
    12781 tgactatgtc gtcgtttaca agaacgaagg aaccgacacg acggtggaaa aagtgaccaa
    12841 gccgaagatc acgcccgtgc agacgaatgc ggaggacgaa tcgcccttcg tgaagaacat
    12901 cgaagcggaa cccgttacgc cgctcacgcc tgtgtatctc gatttcagca aagacgtgaa
    12961 gaaatacagc gtgaagaacg agatgccggt gtactatgcg cagaacaacg aagacagcac
    13021 gttcagcatg tacatcctgt tcgacatgag cgcgaagcgg aataaaatgc tcaagcccgc
    13081 gctcgattac ctgcagttcg ccgggacgtc gacgatgtcc gccgcgcagg tgcagcagga
    13141 attctacaag ctcgcgtgca cgtatttcac ttacgcgggc accgaccaga cgtacgtcgg
    13201 tctttcgggg ctgacgaaaa atttcgttcc cgccatgaag ctgctcgaaa gcctgctctc
    13261 cgacgtgcag ccggacgaag acgcgctgaa gaacaccgtc tcgtccacga tgaaagaccg
    13321 ggagaacgaa gagctcgaca agaacacgat cctcttctcg gccatgtacg attacggcat
    13381 ttacgggaag cattcgccat tctgcaacgt gctcacgaat aaagagctga agaatgtgaa
    13441 agcgaaagag ctcacggacc tcatccacga tctctgcaac tacccgcacc acgtgctgta
    13501 ttatggttcg gagcccgggc aggcgctggt gaatgatctc ggcacgctgc acaaaatgcc
    13561 ggcgcagttc aagccgctgc cgcaggcgga cacgttcgtg cagctgaaag ggaccaacca
    13621 ggtgtacatc atcgaccacg acatgaagca ggccgagatc atcatcctgc agcagggcgg
    13681 atattacgat cccgcgatca agccgcaggc tgcgctctac aatgaatatt tcggcggctc
    13741 gatggcgtcg atcgttttcc agacgctgcg cgaatcgaaa gcgctcgcgt attccacgtt
    13801 ctgctcgtac tatgcgccgt cggatacgac ggaacattat tacaacctcg cgtacatcgg
    13861 ttcgcaggtg gataaattgc ccgaggccgt cgacgggatg atggacctgc tcaacaacga
    13921 catgccgcaa tacccgcagc tgtggagctc atcgcgcgac gcgatcctga aaagcatcgc
    13981 ctccacgcgc atcatccgcg a
//
LOCUS       DASTSF010000199        34245 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1467161,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000199 DASTSF010000000
VERSION     DASTSF010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 34245)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34245)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34245
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1467161"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..885)
                     /locus_tag="VFU15_05725"
     CDS             complement(<1..885)
                     /locus_tag="VFU15_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015907995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEU4717307.1"
                     /translation="MKDYYRILGVAENAPDADIKAAYRRLAKKFHPDVNPGNLYAEEK
                     FKELQEAYDVLSDPGKKRDYDFKRVHQYSFSTHSDFYSGFPREERREPRRREYSPEDL
                     YRARRVHKARLLQHMRKRKKLLVGMIITFVLYLAATAAFENHITKKREEESLALTKKL
                     EDLDLAKPDLKKRDIQNLDSPYDSLFGTGRETWLSPNELVIYNPLSDAVVCAVDAKAH
                     KTIRNEFIHAKNAFVMKALPNGTFYIKVYTGENWSWNKKIPGGMQLGGFTKDEEFFRI
                     DAGPFTLEKPTYGHTDTNT"
     gene            complement(933..2684)
                     /locus_tag="VFU15_05730"
     CDS             complement(933..2684)
                     /locus_tag="VFU15_05730"
                     /inference="COORDINATES: protein motif:HMM:TIGR02231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HEU4717308.1"
                     /translation="MKKIIFLLCLLPVTLLAGDVTQKIESPITSVVVYLTGAEVTHTK
                     TVSISPGRNELHFVGLTSKLIPKSLQFTASGDVSVLAISDHIDYLYGQKKNDEHVKQL
                     TDSLNLMSDNLAITRGQIDAYEKEKQMLDANQGIGGTNNGVVPADLKLSADFYYTRTL
                     EINTELVKLNRKASDQQEVVNRLNWQLTEANQRTNPPMAEVVVLLNSTSTIKFESATE
                     LHYVVSDAGWAPSYDLIADDVSKPIDLKYRAKVFNNTDVDWKNVKMKLSTADPMQTAA
                     APQLDPWTLSFEQNNSSYYNNSGYGNTSGWNYGGNQPTDAGGLANQNLANSMPAQPQR
                     APAQQQYYNQNGNAQVVFNDKQVQQTQQIQYDQIQVSELSAEFDIKLQYDIPADGKPY
                     IVDVTEYNLNATFQYHCVPKMEREAFLMARITGWEDLDLVEGPANVYFGGTYVGQSYI
                     DTRSTDDTLDLSLGRDQKLVVTRTKLKEFNNEKSSATQKKETYSYEIDVKNTRKTAVT
                     VQLQDQIPVSQDAEIIVDASQLSGGTLDPATGIVTWNLNIQPGETSKIVLTYTIKYPK
                     NKVVNTRKFKTTSRAKF"
     gene            complement(2760..4445)
                     /locus_tag="VFU15_05735"
     CDS             complement(2760..4445)
                     /locus_tag="VFU15_05735"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU4717309.1"
                     /translation="MITDGISAHVATQIYEGLVKFDPRTLQILPGLADKWEVDPTGTT
                     YTFHLKKDVHFQDDPCFPDGKGRVMTANDVKYSFELLCRNDGHNPNFSSSFKDRVVGA
                     NAFYTAGASAAEGSLQGVTVIDPQTVQVTLEKPSISFLSVLANPIASIVPKEAVEKYK
                     EKMHVGTGPFILSSIANDTSSLVLVRNDNYYGKDSLGNQLPFLDTIKVSFIDNKKEEL
                     DLFQAGRLDFVWGLNAEAVKVFLPQVISDFKQKPPKFQLDHSSEFVTQIYVFNTTRPP
                     FNNVKVRQAFSYAIDRRHLVDEVLGGEAYGPGLNGICPPALPGYKASEIKGYESIKET
                     DEETAKQKEADRDLARKLLAEAGYPDGKGFPTVKLVLNSGGSKNTRVAEEISNELREV
                     LHVNIEYASVSFQQKLADEQHGQSDMFRSAWVADYPSPETFLSLFYGGDVPDSLSVAS
                     FPNTSRYKNPQFDSLYNAARTAKTTEEANKLFLQAEQIMMNDAPAIILWYDENYRFTQ
                     YRVKNFYTNPMRYLDFSQVYIKESPSKDGKDSAGGGSNTKSDASTEENKEDKH"
     gene            4904..5704
                     /locus_tag="VFU15_05740"
     CDS             4904..5704
                     /locus_tag="VFU15_05740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717310.1"
                     /translation="MKNRFLLLSFAVLLLVFKGYSQSMILLEGTYQGKNVYVQNPFNN
                     NGVGFCVYEVRVNDQITTDEIGSSAFEIDLRNFNLKQGDAVSIKIFHKPDCTPKVLNP
                     EVLKPKSTFNTTSITVDKTDETLKWTTTGESGKLTFVVEQFRWNKWVKVGEVEGLGTA
                     GPNNYSFKIVPHSGENQFRVKQVDYSGQARTSPVAKYTSDVQPIKFYPVKVKDKITFE
                     GGETMWEIYDQYGNIVKKGFGSTVDCTGLAKGGYFLNYDNTTAEFFKQ"
     gene            5764..6168
                     /gene="mce"
                     /locus_tag="VFU15_05745"
     CDS             5764..6168
                     /gene="mce"
                     /locus_tag="VFU15_05745"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007647197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HEU4717311.1"
                     /translation="MKKIEHIGIAVKNLDEAEKLYAKLLGSPSYKRETVESEGVITSF
                     FRTGENKIELLGSLTENGPVAKFLEKKGEGIHHIAFDVEDIRAEMKRLHAEGFVLLNE
                     EPKKGADNKLICFVHPKSSAGVLIELCQEIRE"
     gene            complement(6239..7924)
                     /locus_tag="VFU15_05750"
     CDS             complement(6239..7924)
                     /locus_tag="VFU15_05750"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEU4717312.1"
                     /translation="MKTIYLSFIAMLGACTMLSAQTGRQPIPAGGFLQTIRASQSETH
                     NDARTPSAPTVQYGNDIIIQNAPAIDQRRVRISVAFNGWVYAAYSTYDSAGVAGGITI
                     RSSRDNGMTWTTVDSYSPQNIVYPAFDIVVCGTDTNNLTLYLAGVNHNTTSGNYVLFV
                     DRYNATTGSFIGSNYNLQNGTRRIYDVALASDYRFPAVGASPYSVGLLYSAYSSSVDS
                     MVFLGSVDGGGTWSVRQSVMTTGYYFRNVSLAYGRSASASNGRYFGAWEMLTSSGDRT
                     GHIYTSRSTSTVNDAWIPPVNLDSISSSMINLCCNPEIAVQFNGTDNDSSSVTAVVLC
                     QRDFYGNGTDYDLLGFYNKRAHYTNFWNRLDIVNSGENDLQPDISYDPGYDNFLTVYY
                     DSTDGKLPYCVNGNNLVAPNTWNYITPQYNDMTGNLRAAWPRVEINPMLTQTAHAWIA
                     EGAGSNGVAMFDAEYLTSSISEAGGASITGLFPNPSSSSFTLNYSTSDNNAVLFTVTN
                     TLGEVVETRRPDGSGPGNHTEQFDVSGWANGVYFVTMLNNGKSSTARIVVAHP"
     gene            complement(8020..8640)
                     /locus_tag="VFU15_05755"
     CDS             complement(8020..8640)
                     /locus_tag="VFU15_05755"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEU4717313.1"
                     /translation="MLRNIIFDLGGVIINLDPAATEQAFVRLGMTDFSSQYSTAKQSG
                     LFDDFDKGKISPEAFRTELKKYFPAPVTGRAIDEAWNAMLLDVPPERLELLAHLGKRY
                     RLFLLSNTNEIHVAAFSAYLQREYGFSDFSGFFEKWYYSCRMGMRKPDREIFLRVLDE
                     NKLDPAETLFIDDSMQHVKGAAETGISSLWLAPGKTILDLVREKVF"
     gene            8697..9680
                     /locus_tag="VFU15_05760"
     CDS             8697..9680
                     /locus_tag="VFU15_05760"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEU4717314.1"
                     /translation="MKGKPVYTISGLVLAVASVPFVFFLHLPLAGAILAALGYFVAMK
                     EFSKFTGPLQFFTQLFAGLMTGTALEYPFHVFPFLLLTMFFATLSTFGRIMFFRFFAY
                     TGKTWFEPLMLFLAIGCHVAGNMSAHSDWAGWLLPGPAIIFAGIITWGIIKDKRQLLA
                     KTMKGYKIAIGSPAPPFSLEANDGTTVSLSDFYGDRNLLLIFVRGDWCPGCHMMLRTY
                     QKNSEQFRQKNIFVLSIGPDPVGVNKEMVERLGLDFKVLADEGQKVAMKYGVQLQEYD
                     NAFAEKYDEGIPLPASFLVDKTGIVRYVSRPDKVGEFLNPSLIFPIVEKLA"
     gene            9690..10670
                     /locus_tag="VFU15_05765"
     CDS             9690..10670
                     /locus_tag="VFU15_05765"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin family protein"
                     /protein_id="HEU4717315.1"
                     /translation="MYFFLAIFSFLFSATSVVLCVLKPDLQPWMFPVPAFLGWITGLL
                     LIKTRTAAVDIAANICGLISLGFSLDQAGASFPLFTVTLVVLMLTYSSRTLFIRNIGY
                     VKQTWIEPVTLLTGIALFVFVNVVKHPGWYGWAFPAPIVLFGIVYYSSARIETKAFRR
                     GAANELKVGIGSKAPDFALNDENGKSISLAEILKTQPVLLLFVRGDWCPTCHIMLRTY
                     QRESDHFKRKNVMVLAIGPDPQGVNKAMVEKMSLSFHVLSDDRQEVLSLYGIQFLEPI
                     MGQKFEVGSPLPASILIDRKGVIRHICRADRAGEFLNPLQIFTVLEKMDT"
     gene            10667..11995
                     /locus_tag="VFU15_05770"
     CDS             10667..11995
                     /locus_tag="VFU15_05770"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEU4717316.1"
                     /translation="MITAVIISGIIALACAGFSVYALLSRNRMRSAFDGLNKQINNYQ
                     SIIDQANDAMIVIDIVDGRIHQSNPGAAQLLGYSQEELSKKSLFDLHPKEDLERSSMI
                     VADVWEKGGLIYKDIPFVTKSGEILPVECSAKVAPFAGRPAIVIYARDIRERLKLEGE
                     IQLQKNIIEQKNKDITDSINYAKRIQEAILPTEEQMKEVAPDHFVFYRPKDIVSGDFY
                     WATSVTTTDPSRPDQKKYSLIAAIDCTGHGVPGAFMSIVGHTILEQTITDPDVNSPAQ
                     ALDFLNRGVIKTLKQKANDDFSVKDGMDMAFCAIDPEKRILNFAGANNPVYIIRGGQL
                     IELSGDKQPIGAYMKDVAPFTDKSFQLEKGDCIYIFTDGIADQFGGPKGKKFKYKQLQ
                     QLLVNIHSQNMDQQKKTIVKTVEDWMSFPTPSGGTFEQTDDMLIIGVRVS"
     gene            12010..13944
                     /locus_tag="VFU15_05775"
     CDS             12010..13944
                     /locus_tag="VFU15_05775"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU4717317.1"
                     /translation="MTSEERAGIEKMLAGGVSGRERVDLLNKVAVEYRFSEPQRFCDY
                     ATEACTAAEEIGYTEGLARAERHLGYFYEQQADYPKTLSYCSKALEHYKSVPGLSTEI
                     IATNNLVAKVYANLGDFTKGLEYLYESLRLSEETGNKTAETNTLNSMSVLYQRLGNAA
                     KAEECASRGLTLAAELTDQRVLGVTMVNLGNAYGMTKDWTRALEQWKTSLSIFEKLGD
                     EDLQSSVLGNMGITHMNLGEYDRADEYLSRCLAIKKQTGNRYDVARSLQNLASVSSSM
                     KQYRKSLDYCREGLELAALVDARSLEYQLWYEMAAAYKGLGDFENALTCFEKYHSLEK
                     EIFTEEMRLKTDALQLRFAIEKSEKEKEIYRLKNIDLAEANEKITIQKEVIEEKNRDI
                     TAGIRYAQRIQEALLPQESALRHGFSEAFVAYFPRDIVSGDFYWSLISEDAVLFAAAD
                     CTGHGVPGALMSVMGAAFLSEIVNERGVTDPAEALNLLRLKVITALQGKEERDATESG
                     LRDGMDIVLCRYEPASRLLRFACANNPLWLLRKGNITEFGADKFPVGLHHGAMMNFTT
                     RQVTLQPGDQLFLFTDGFADQFGGTKEKKFGYRRLRELFVSIAGLAAAEQKAALEKAF
                     REWKGENDQVDDLLVIGVKA"
     gene            complement(13951..15237)
                     /locus_tag="VFU15_05780"
     CDS             complement(13951..15237)
                     /locus_tag="VFU15_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014018668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEU4717318.1"
                     /translation="MHRAPGFIGILIFVVFLLLIDFYVFSGVKAITSGYSSRTKKIVQ
                     WTFWSINAGLYLWMFALVLFTSFSKGFPRSFMMFVGAWVLFFIPKLVFSLFLLGEDVY
                     RLLRGFYSLGHNVLTKNDPMEFGSSRRKFISTVAAATAAIPFAGIIHGLTAGKFRFRV
                     RREEVFFPDLPDAFDGFTITQLSDIHIGSFDPDSDRDEVRRGIAMANAEKSDLFVFTG
                     DLVNNMASEMDPWMNEVKQLRAPYGQYSILGNHDYGMYLTWPEQERLKSFNRLCEIHG
                     ELGFRLLRNEHTVIEKDGQQLYLLGVEDWGRGFLHEGDLDKALEGVPENAFKILLSHD
                     PSHFDEIVSQHPTHVHMTLSGHTHGAQFGVEIPGVKWSPVQFAYPHWAGLYNEAKRYI
                     YVNRGFGFLAFPGRVGIWPEVTVLTLRKGNSAPVST"
     gene            15399..18677
                     /locus_tag="VFU15_05785"
     CDS             15399..18677
                     /locus_tag="VFU15_05785"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HEU4717319.1"
                     /translation="MKHLLSFFLSLALSSLAAQETGQFRHYTTEDGLSQNSVYSIVQD
                     RQGFMWMATQDGLSRFDGYRFEVFHPDAADSNSLSNNSVRCLLVARDGLIWIGTRDGL
                     NVFDPVKKRFSHFFKAKNGLISGNILSLAQDSSGNIWVSSLHEGVSVYNQQKKTFSYF
                     RHDDSNPNSLCGNDVRGMCCDHNGHMWLVTWSNGMSEYDPSSGKWTTFSSGGGPGQLS
                     HPNSRGAICADSKGRIWIGTWGRGLDMWDPSKHEMFSEDRNTPELPQMRSGLVWSVYE
                     TRNGHIWFTTAEKGVYDFDPATGHYRNFIHDADDPRSINDNSIWSITEDRSGLIWTGA
                     WQGGVNVYDPRMERFALFRVNPNDSNSLPANVTWTFCTDGAGGIWMGTAAGPVHYDPV
                     TKQFRQPPLDDPDHKVLYKNAPTPRSNIQALCRDGNGNIWMGTAGAGLHCYDPVTKTY
                     DLYMPEENNDASLTGNTVNSLTTDEEGNVWVCCGSSPFQRFDPVRKAFENFPFAKGDS
                     IRPNDGPVCSISGGPGKMIVGLTGGEVFELDVKTRKYTLIWKSPSDVSVTSLCRDKLG
                     SLWIGMGGSGLMFINGKTQTLFTDKDGLPNDFINGIIPGDNGELWLSTNRGICRFDPV
                     KHDVRIFTTEDGLQANEFNQGGAFRSHDGRIWFGGVNGISAFFPDQITPNTSPAAAII
                     TSLTVLNKKIPLDSDITFTKEIRLSWRDYFFSFEFSGLEFTNPERNQYKYMMEGFDDD
                     WVDAGTRRFATYTNLDPGEYTFRVKASNNDGNWDGREARIHIIITPPFWKTKWFYTLC
                     GIFMLLSVVGYIRWRERKLRKEKEVLESKVTERTYELQQEKEKVTAAHKDIRDSINYA
                     QRIQYALLSHDELLKKNLPEHFIFFRPKDVVSGDFYWATQQGPVFWLAVCDSTGHGVP
                     GAFMSLLNISYLNEAIIEKKMTEPGAVFDYVRSRLIENISQDGGKDGMDAILLKIDHE
                     KNTITYAAAHNAPVVIVNGNTLVTETDKMPVGKGERTDHFKTRTLETVPGEMIYLFTD
                     GCADQFGGPAGKKFKEVNLRKNLAEISALPAGEQRSRLEKTFEDWKSHHEQVDDVLVI
                     GVRI"
     gene            18816..18889
                     /locus_tag="VFU15_05790"
     tRNA            18816..18889
                     /locus_tag="VFU15_05790"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18850..18852,aa:Ile,seq:cat)
     gene            complement(19497..19886)
                     /locus_tag="VFU15_05795"
     CDS             complement(19497..19886)
                     /locus_tag="VFU15_05795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717320.1"
                     /translation="MPAMNYTAGIKPYKAVMQTYSPNIRPYRPYRNFYENNLQVKTVN
                     GPSGKKQSVISDRNRSVADYLASGQFINGIYRLAMDKETSGAPGKNRKRFLQTAAKKI
                     LEPLRSLFMRVKAGFGLLPGKEVPGSR"
     gene            20241..20912
                     /locus_tag="VFU15_05800"
     CDS             20241..20912
                     /locus_tag="VFU15_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018624541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2589 domain-containing protein"
                     /protein_id="HEU4717321.1"
                     /translation="MAETALVSMAQQFTGLPMDALIGAPLNAAANANAAMAITQTKFM
                     LDTCFSRQGDDKTGYNYTPIMIKMSLTRGVITPGDPNANPPTKTDIQSVTTTFDLPLL
                     TIIPLNSLGVDNVNVTFEMEVKSSFAEEQSEATEKNIQAETSFEVKVGWGPVSATVKG
                     SASYDQKDSSNHSTHYEKSNSAKYTVLVHAGQLPLPKGVNTIIEAYTMAIQPVTMPVV
                     TPPKS"
     gene            21021..21656
                     /locus_tag="VFU15_05805"
     CDS             21021..21656
                     /locus_tag="VFU15_05805"
                     /inference="COORDINATES: protein motif:HMM:NF023085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2589 domain-containing protein"
                     /protein_id="HEU4717322.1"
                     /translation="MANFQTKTLDPSDYSGSDIAAIIGAPLVAAAHANAMMAREQIKF
                     LMSFCFSKTGEVYEPVMIRMSMTKGVLTPSDEKDKPPFIQQLTTTFEIPLLTVIPINS
                     LAVQSMEVNFDLEVTSHINVPSSRNSPGNLKPDHKSVRMMGKISSSPGDGQGTDKDYY
                     DKTGASTLKVNIKSGPLPLPKGLASVLEVYSKNIHPVELNPGADTAQTDKK"
     gene            21714..21932
                     /locus_tag="VFU15_05810"
     CDS             21714..21932
                     /locus_tag="VFU15_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007211171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717323.1"
                     /translation="MSDQLSIPCPDCKTPIPFDPRMLIQGAQFSCPKCKAFLGLASES
                     KEIVRESLDKFDKMKSEVLKAKGDTTGF"
     gene            21944..22450
                     /locus_tag="VFU15_05815"
     CDS             21944..22450
                     /locus_tag="VFU15_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007211170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2589 domain-containing protein"
                     /protein_id="HEU4717324.1"
                     /translation="MISFKAFIEGINSAIVAANDDLMNKHEEILDTYFEKTGEDKTDG
                     HEGHSTLRPKSVCIQYPKMEVDGSITMIDVDVPLVTLIPIEFTKIDKVKITAQFGLQV
                     INNDLQIIFSNEAPKKKWSLFGGSKTDDKTPSPGQLEITIRPSETPEGLKKMIDGYDK
                     VLRAQIPH"
     gene            22493..23017
                     /locus_tag="VFU15_05820"
     CDS             22493..23017
                     /locus_tag="VFU15_05820"
                     /inference="COORDINATES: protein motif:HMM:NF023802.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="papain-like cysteine protease family protein"
                     /protein_id="HEU4717325.1"
                     /translation="MPSLSGFPAAKQEDPNSCWACASREISNYYKSLGKGGKNEMYSS
                     DKELAAAWSKITGKPGNADISIQQSASAALGDLGYTNSADSAPVPTVGEITDAINDKK
                     PLLAIVGSTDPKGKRKLGYQQGHWVVITGISDKGDLIEVFDPDSGKIETVAYSSQVYK
                     SGMFWQNTSYVDGQ"
     gene            complement(23034..24656)
                     /locus_tag="VFU15_05825"
     CDS             complement(23034..24656)
                     /locus_tag="VFU15_05825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717326.1"
                     /translation="MKGTRIFFFALLALTIFCVKANAQAFLIDEEFNAAPAAPAGWTF
                     TSISSTYTSSANYGRSSPSVKFDGSGEIALSPAWSGSADEVTFFTRGNGSTPTTDNFL
                     IEGWNGSSWTTVGRAYPQTTAKVFVFPLASNVTRLRFTYTKVVANCAFDDVKVRAAGF
                     CSGQLSITGIMADACGSDEGYDEFVSCANGGPAMNLSDLEITFSDVGSNGGCTYCGTT
                     GNPCDNFFVNNAATNTYIASLNTMAGCGTLFMAPPGNVIPAGGNFIVFAGAPPTYTYN
                     FSNLCSTGLTYYAVFANNTSDNNGRFGNNGACTGQACPRDAMLRNNATGCVDTNYYNR
                     GAIPNNNGEMAYFGSAGHSATYINTGCTTFTVLPIELSVFSGEVQGGSVVLSWTTESE
                     NNNSFFTVERAGADGNFTAIGTVNGSGTTSQPVEYEFTDVTPLPGQNYYRLRWTDFNG
                     ASIVSAIISVTAETDRVYANAWYDGTGIHYRSANWSSEMHISIFDSSGRLVYETVSGN
                     GDNVIPLAVPSHDMFLVVFGDEENRITQKVAW"
     gene            complement(25053..25625)
                     /gene="purN"
                     /locus_tag="VFU15_05830"
     CDS             complement(25053..25625)
                     /gene="purN"
                     /locus_tag="VFU15_05830"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEU4717327.1"
                     /translation="MKRIAVFASGTGTNARNLIEYFEPHHNAQVTLLVCNNPQAGVIS
                     IAKEHGVEVLLTDREQFFSEHSIVPQLKERGIDLIVLAGFLWLIPPAVISAFPGKIVN
                     IHPALLPKFGGKGMYGKKVHEAVLASGEKVTGVTIHFVNEKFDDGKHIAQFTCPVLPG
                     DTPETLSARVQQLEHAHFAETIDRNVIRLL"
     gene            complement(25654..26439)
                     /locus_tag="VFU15_05835"
     CDS             complement(25654..26439)
                     /locus_tag="VFU15_05835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717328.1"
                     /translation="MKKLQWLTCACFGAFLFSCGNDVKKETVSGEAKKDSVAKTENRQ
                     PVAESLYGDTLEPRDPSLVLFRFDEKLSGRKPVLLKAGEVRKRFWPLDKTADPSALFT
                     FARYFELDSIRKTGKEYEGDVGDIMQVDIDVLDTLKNAGPFCAVAWRMHFVSYEACPF
                     SSGDYFMITTYGKDGKAIRSYMIAQDISGADAPVFFHSQQRTNVYTDGTFRSCSTDTM
                     GDEGEDKPVYGVVKTVSTGRIDPAGMIFSREISRDSTGTFDFN"
     gene            complement(26507..27211)
                     /locus_tag="VFU15_05840"
     CDS             complement(26507..27211)
                     /locus_tag="VFU15_05840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717329.1"
                     /translation="MSAYHGRVRPAFSPVVSPLVPATFATKSKKIFAFWVASAIVGGI
                     ASAIGSGIAGAPGMYTGIGIGALMLVLTTIAALRSRHAKCPYCAFDVGSTTDVTISMK
                     DPDLQVACPRCCEWLISDKGVLRAFTENDTQAKSKFTCPVFVTSLWPDECIVCGSPVV
                     KYAQAKKTSVSVGRLLAGHLSVASGSVSNIPYCADHQDAVSLDVNGSELQLTFGDFGA
                     MRRYLAMNPNRIRYKK"
     gene            27350..28012
                     /locus_tag="VFU15_05845"
     CDS             27350..28012
                     /locus_tag="VFU15_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009580682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEU4717330.1"
                     /translation="MNFPFIPYTHERFPDEEMIARSQSFSEWMDKRRTVRDFSSEPFP
                     FEVLENIVRTASTAPSGAHKQPWTFCIVTNSELKKKIREAAEKEEYESYNGRMTPEWL
                     EDLAPLGTDWRKPFLETAPALIVVFRRPYEIVNGVKKNNYYVNESVGLACGFLLAAIH
                     HAGLVALTHTPSPMNFLAQLLERPENEKPFLLIPVGHPAGNCTVPDIRRKPLENVLVA
                     YK"
     gene            28094..28330
                     /locus_tag="VFU15_05850"
     CDS             28094..28330
                     /locus_tag="VFU15_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005779510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEU4717331.1"
                     /translation="MSDISAKVKAIIVDKLGVDEKEVTETASFTNDLGADSLDTVELI
                     MEFEKEFNIAIPDDQAEKINTVGEAIKYIQEHAK"
     gene            28359..29612
                     /gene="fabF"
                     /locus_tag="VFU15_05855"
     CDS             28359..29612
                     /gene="fabF"
                     /locus_tag="VFU15_05855"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009055229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEU4717332.1"
                     /translation="MQLKRVVVTGLGAITPLGNSVQDYWSNLVAGKSGAATITRFDAS
                     KFKTQFACEVKGFKTEDYFDRKEARKLDPFSHYGIAAADQAVRDAGVNADSIDKTRVG
                     VIWGSGIGGLQTFQDECFGFAKGDGTPRFNPFFIPKMIADICSGHISMRFGFHGPNFT
                     TVSACASSTNALIDSFNYIRLGKANVIVTGGSEAAICEAGIGGFNAAQAMSTRNDSPE
                     TASRPYDKDRDGFVLGEGGAALVFEELEHALNRGAKIYCEVAGGGLSADAYHITAPHP
                     EGLGAALVMRNALEDAGITAADIDYINTHGTSTPLGDVAELKAIQKIFGEHAYKLNIS
                     ATKSMTGHLLGAAGAVEAMATILSVVNNTIPPTINHFTDDPEIDPKLNLTFGKAQQRE
                     VRAAISNTFGFGGHNAAVVVRKFSA"
     gene            29621..30349
                     /gene="rnc"
                     /locus_tag="VFU15_05860"
     CDS             29621..30349
                     /gene="rnc"
                     /locus_tag="VFU15_05860"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010599622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEU4717333.1"
                     /translation="MFSAFRALFSSGPDKAFRKTLRNLLGFWPDNIRLYRKAFTHSSA
                     ANLAKDREQPRESYERLEFLGDAILSAVIADYLFKKFPYRDEGFLTKLRSRIVSRQTL
                     GKLAVKFGIDKFVQAESGLAGRSNSINGDVFEALIGAIYLDKGYDFTSDYINERILGF
                     HLDIDEIEATDTDFKSKLIEWAQKNRKDLRFNLAGEVGTGQHKQFIIEVTIDGVVHGR
                     SQHLSKKRAEQEAAGLCLAELKLS"
     gene            30718..31299
                     /locus_tag="VFU15_05865"
     CDS             30718..31299
                     /locus_tag="VFU15_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014681050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEU4717334.1"
                     /translation="MNDHELIAGCIRQDRACQEALYKRFYGKMMGVCLRYAKSRDEAR
                     DMLQEGLIKVFTSIRNFQGKGSFEGWIRRIVVNTAVDHLRRNKHEYMIVSTIYAREGD
                     IPDQVEEMEEDNLIANLSEEEILASVQQLSPAYRTVFNLYVMENFSHREIADQLGISE
                     GTSKSNLSKARYQLKKNLTNLIKNTDGERTFTR"
     gene            31316..32020
                     /locus_tag="VFU15_05870"
     CDS             31316..32020
                     /locus_tag="VFU15_05870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717335.1"
                     /translation="MHDRLSDYEAPYNGADWDAMDRSLDALPKTNRFQWKFSLNTLLI
                     AVGVASLISLGVFLASGHSAAPGKPQAVAQPATDNNLPSKNTSVVTNAPPSQVQTVNM
                     ADVQEVSSPGLVTGNSSNPSPGNTDVVQNTSSARKTKTKKDSGLLFGDQIDPAKGFIH
                     RTQEDQKLIDQYANRGTPSVYFDKENGQLKEIIINRDSAHVTGIKKDYAPADTTAKNT
                     PRGAPVETEQHGFDFN"
     gene            32145..33113
                     /locus_tag="VFU15_05875"
     CDS             32145..33113
                     /locus_tag="VFU15_05875"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HEU4717336.1"
                     /translation="MKTLVRIFSVAVLLASAADALHAQDPEFTQFYANPLYLNPALAG
                     SKICPRFNLSYRAQWPGIYGTYSTIGASFDKYAYKVKGGIGVTVVEDRAAKGTLNTTG
                     VGLIYAPVVPLGKDISVSAAIQAGYWQKTVDWSKLTFGDQIDPHSGFNLLTQEPSPQT
                     QREGDFDLGAGLLFSSRNFYAGGAVHHILEQNESFLGGSSPLPRKYTLHAGGMIPLTH
                     DRYEGDSYISPNILYQKQGDFQQLNIGLYAKKDAIVGGLWYRGNDSFIMLIGIEAGQL
                     HLGYSYDVTVSKLSNASAGSHEVTMGYQMSCKPPKKKYRPVNCPGW"
     gene            33314..>34245
                     /locus_tag="VFU15_05880"
     CDS             33314..>34245
                     /locus_tag="VFU15_05880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717337.1"
                     /translation="MRIFTRERLIALGLFFVFLFGMTQFSRKMHYAVGMGTCIWSDGI
                     GYYEYLPALFIRHDVNHIGWGMPLPNGNRFDKYATGVAILETPFFLAADAATVLNHRE
                     RTGFSSYYGYGVLFADLFYTFAGFIFLFYTLRKWFSLRTVFFSIILIALGTNLFYYGF
                     AEPSMSHAYSFFAFAGFLFYCRKFHEEGRRSRHLHAVGFFLGLATAIRPTSIVIGIFF
                     LLYGVYSGKDFRERLKTFFSDRRIIPALLFWGVLLFLPQMLYWHSTTGKWFTYSYGYY
                     DEGFTYWKSPHFFDVLFGPWCSWIPYTPVMIFFFP"
BASE COUNT         8244 a         9165 c         8905 g         7931 t
ORIGIN      
        1 cgtattcgtg tcggtatgcc cgtacgtcgg tttttccagc gtaaaaggcc ctgcgtcgat
       61 cctgaaaaac tcttcgtctt ttgtaaatcc cccgagctgc atgcccccgg ggatcttctt
      121 attccagctc cagttctcac cggtatacac cttgatgtaa aacgtcccgt tcggaagcgc
      181 tttcatgacg aatgcgtttt tcgcgtgaat gaattcattg cgtatcgtct tgtgcgcttt
      241 tgcgtcaaca gcacacacaa ccgcatcgga aagcgggttg tagatcacca gctcgttcgg
      301 ggaaagccag gtttcccggc ccgttccgaa aagactgtcg tacggtgaat cgaggttctg
      361 aatatcccgc ttttttaaat cgggtttggc caggtctagg tcttcgagct tcttcgtcag
      421 cgccagcgat tcctcttctc tctttttcgt gatgtgattc tcgaacgcgg ccgtggcggc
      481 aagatagaga acgaacgtga tgatcattcc cacgagcagc tttttccgtt tgcgcatgtg
      541 ctgcagcagc cgcgccttgt gcacgcgcct tgcccggtaa agatcctccg gcgaatattc
      601 ccgccgccgc ggttcccttc tttcctcccg gggaaagccg gaatagaaat cggaatgcgt
      661 ggaaaaggaa tactggtgta cccgtttgaa gtcgtaatcc ctttttttcc cgggatccga
      721 aagaacatca tacgcttcct gcagctcctt gaacttttct tccgcgtaaa gattcccggg
      781 gttgacatcg ggatgaaatt ttttggcaag acggcggtac gccgctttga tgtcggcatc
      841 cggcgcattt tctgcaacgc caagtatgcg gtaataatct ttcacgtaag ctgtaaatcc
      901 gttccgttac aaaaacagtt cccggccggt tatcagaatt tcgccctcga cgttgttttg
      961 aacttgcgcg tgttcaccac tttgttcttc ggatatttga tcgtgtacgt gagcacgatc
     1021 ttgctcgtct ccccgggctg tatgttcagg ttccacgtaa caatgcccgt ggccgggtcg
     1081 agcgttccgc cggaaagctg tgaagcgtcg acaataatct ccgcatcctg cgaaaccggg
     1141 atctggtcct gcagctgaac ggtcaccgct gtcttccgcg tgttcttcac atcgatctcg
     1201 taggaatatg tttccttctt ctgcgtcgcg gatgattttt cgttgttgaa ctctttcagc
     1261 ttcgttcgcg taacaacaag cttctggtcg cggccgagcg aaagatcgag cgtgtcgtcc
     1321 gtgctccgcg tatcgatgta cgactgcccg acgtatgttc cgccgaaata aacatttgcc
     1381 ggaccttcca ccaggtcgag atcttcccag ccggtgatgc gggccatgag aaacgcttcg
     1441 cgctccattt tcggaacaca gtgatactgg aacgtcgcgt tgaggttgta ctccgtcaca
     1501 tcgacgatgt acggttttcc gtcagcagga atatcatatt gcagcttgat gtcgaattcg
     1561 gcgctcagct ccgagacctg gatctggtcg tactgaatct gctgcgtttg ctgcacctgc
     1621 ttatcgttga aaacgacctg cgcattcccg ttctggttgt aatactgttg ctgcgcggga
     1681 gctctttgcg gctgcgccgg catggaattg gcgaggttct ggttggcaag accgccggcg
     1741 tcggttggct gattgccgcc ataattccac ccgctggtat ttccataacc ggaattgttg
     1801 taatacgatg aattgttctg ctcgaagctc agcgtccacg gatcgagctg cggtgccgca
     1861 gccgtctgca tcgggtctgc cgtggaaagt ttcatcttca catttttcca gtcgacatcc
     1921 gtgttattga aaacttttgc gcggtatttc agatcgatcg gcttgctgac atcatcggca
     1981 ataagatcat agctcggcgc ccagccggca tcgcttacca cgtagtgaag ctccgtcgcc
     2041 gattcgaact tgatcgtgct ggtcgagttc agcagcacaa ccacttccgc catcggcggg
     2101 tttgtgcgct ggttcgcttc cgtcagctgc cagttgaggc ggttgacgac ctcctgctga
     2161 tcggaagctt tccggttcag cttcaccagc tcggtattga tctcgagcgt gcgcgtgtaa
     2221 taaaaatccg ccgacagctt gaggtcagca ggaacaactc cgttgttcgt tccgccgatt
     2281 ccctggttcg catcgagcat ttgtttttct ttttcgtagg catcgatctg cccgcgcgtg
     2341 atcgcgaggt tgtccgacat gaggttgagc gaatccgtca gctgctttac atgctcgtca
     2401 ttttttttct ggccgtacag gtaatcgatg tgatcgctga tggcgagaac ggagacatcg
     2461 ccgcttgcgg tgaactgcag gcttttcgga atgagcttcg aggtgagccc gacaaaatgc
     2521 agctcgttcc ttcccggtga aatggaaacg gttttcgtgt gcgtcacttc cgcaccggtg
     2581 aggtaaacaa ccaccgacgt gatcggcgat tctatttttt gtgtaacgtc tcccgcaagc
     2641 agtgttaccg gaagcaggca gagaagaaaa ataatttttt tcatggggaa tagttttggg
     2701 ttggaacaaa aacccctgtc cgcaaggcga acaggggttc ggtgaaatcg ttttacggat
     2761 cagtgcttgt cttctttatt ctcttccgtg ctggcgtcac tttttgtatt cgagccgcca
     2821 cctgccgaat ctttgccgtc tttggaaggg gattctttaa tataaacctg tgagaaatcg
     2881 aggtaacgca tcgggttggt atagaaattc ttcacacggt attgcgtgaa acggtagttc
     2941 tcatcatacc acaggatgat cgcaggcgcg tcattcatca tgatctgttc cgcctggagg
     3001 aaaagcttgt tggcctcttc ggttgttttc gccgtacgcg cagcattgta aagactgtcg
     3061 aactgcggat ttttataccg cgatgtattc gggaacgacg caaccgaaag actgtccggc
     3121 acatcaccgc catagaagag ggaaaggaac gtttccggcg aaggataatc cgcaacccac
     3181 gcggaacgga acatatccga ctggccgtgt tgttcatcgg caagcttttg ctggaacgaa
     3241 acacttgcat actcgatgtt gacgtgaagc acttcgcgca gctcattcga aatttcttcc
     3301 gccacgcgcg tgtttttcga tccgccgctg ttcagcacga gcttcaccgt ggggaaacct
     3361 ttcccgtcgg ggtagccggc ttccgcaagc agcttgcgcg caaggtcgcg atcggcttct
     3421 ttctgcttcg ccgtttcttc atcggtttcc ttgatggatt cgtatccttt gatctcgctt
     3481 gctttgtaac cgggaagcgc gggcggacag ataccgttga gtcccgggcc gtacgcttca
     3541 ccgccgagaa cttcatccac aaggtgtcgc cggtcgatcg catagctgaa cgcctggcgg
     3601 actttcacat tattgaaagg aggacgcgtc gtattgaaca cgtagatctg cgtcacgaat
     3661 tccgaagaat gatcgagctg gaatttcggc ggcttctgct tgaaatcgga aatgacctgc
     3721 ggcagaaaca ccttgaccgc ttccgcgttg agtccccaca caaaatcaag acggccggcc
     3781 tggaaaaggt cgagctcttc ttttttgttg tcgatgaaag acactttgat ggtgtcgagg
     3841 aacggcagct ggttgccgag ggaatctttc ccgtagtagt tgtcgttgcg gacaagcacg
     3901 aggctgctcg tatcattggc aatggacgaa agaatgaacg gccccgttcc gacgtgcatt
     3961 ttttctttgt acttctcgac agcttctttc ggcacaatgc tcgcgatggg attggccagc
     4021 acgctcagga acgagatgct cggtttttcc agcgtcacct gtacggtttg cggatcgatc
     4081 accgtcacgc cctggagact gccttccgcg gcagaagcac cggcggtgta gaatgcattg
     4141 gcacccacca cgcgatcttt gaacgaggaa gagaagttgg ggttgtgccc gtcattccgg
     4201 caaagcagct cgaatgaata cttgacatcg tttgccgtca tcacacggcc ttttccgtcg
     4261 gggaagcagg gatcgtcctg gaaatgaaca tcttttttga gatggaaagt atacgtcgta
     4321 cccgtcgggt cgacttccca tttatcagca aggccgggca ggatctgcag cgtgcgcgga
     4381 tcgaatttta ccagtccttc gtatatctgg gtggctacgt gagcagaaat cccgtccgtt
     4441 atcattgtcg ggaaaagcgt ctggattttg tccgtttcgt taaaacggag tgttccgcca
     4501 taaacacgtc cgcctttacc ctccttgtgt gtaacgttat tgtcgttacc gcaagacccc
     4561 atcaaaaaga ctaaagggag aaacagcagg agttttctca tatatgcgta attgataacg
     4621 agttagttgc cgatttatcg ggcgaagata cgaattcgtc gttcattgca taaggttttt
     4681 gtcaaattat gtggcttcgt tttcgcgcgc aaatcccttt gacattccat attatcttca
     4741 atccgctgtt ttacagtttt ttccaagcac cttcattggg ctgggcgaag aatacgcgga
     4801 attgatcgaa aattaatagg gttttatttt gaattttctc agtaacacac taatattgcc
     4861 cgtcaaattc ggaggtgcac cccctaaatt gcgcattcgt taaatgaaaa atagatttct
     4921 gctgctgtcc tttgctgttc ttctccttgt ttttaaggga tacagccaat ccatgatcct
     4981 tcttgaagga acttaccagg gaaaaaacgt ttacgttcag aaccctttta ataacaacgg
     5041 cgtaggtttc tgcgtgtatg aggttcgtgt gaacgaccag attacaacgg acgagatcgg
     5101 ttccagcgcg tttgagatcg acctgcgcaa tttcaacctc aagcagggcg atgccgtttc
     5161 catcaagatt ttccacaagc ctgattgcac tcccaaagtg ctgaaccccg aagtgcttaa
     5221 acccaaatcc actttcaata cgacttccat caccgttgat aaaacggatg aaacgctcaa
     5281 atggacgacc accggcgaat ccgggaagct gacgttcgtt gtggagcagt tccgctggaa
     5341 caaatgggtt aaagtcggcg aggtggaagg cctcggaacg gccgggccca ataattattc
     5401 attcaagatc gttccgcatt ccggcgaaaa ccagttccgc gtcaaacagg tggattattc
     5461 cgggcaggcg cgcacatccc cggttgccaa atacacatct gacgtgcagc cgatcaagtt
     5521 ctatcctgtc aaggtcaaag acaagatcac tttcgaggga ggggaaacca tgtgggaaat
     5581 ttatgaccag tacggaaata tcgtgaagaa aggatttgga agtaccgtcg actgtacggg
     5641 ccttgccaaa ggcggctact tcctgaatta cgacaacacg actgctgaat tcttcaagca
     5701 gtaaattcat aaaccgacag acttccgatc ccgccaaagg cgggatcatt tttttccggc
     5761 cacatgaaaa aaatagagca catcggcatt gccgtaaaaa acctggatga agcggaaaag
     5821 ctctacgcaa agcttctcgg atcgccctct tataaacggg aaactgtcga aagcgaaggt
     5881 gtgatcactt cttttttccg taccggcgaa aacaagatcg aattgctggg atcgcttacg
     5941 gaaaacggac cggtggcaaa gttcctggaa aaaaaaggcg aaggaattca tcacatcgcg
     6001 ttcgatgtgg aagatatccg ggcagagatg aagcggctgc atgcggaagg atttgtcctg
     6061 ctgaacgaag agccgaaaaa aggagccgac aacaagctta tctgctttgt gcatccgaaa
     6121 tcatccgcgg gcgtgctgat cgaactgtgc caggagatcc gggagtaatt ccgggagcag
     6181 aaaaaacaaa aaagcggatc gcagtgatcc gcttttcgca tttaagaacg aagcttgttt
     6241 acggatgagc gacaacgatt cttgctgtcg agcttttccc gttgttgagc attgtcacga
     6301 aataaacacc gttcgcccag cccgaaacat cgaactgctc ggtgtggttt cccggacctg
     6361 atccgtcagg ccggcgtgtt tccacaacct ctccgagcgt attggtaacc gtgaacagca
     6421 cggcgttatt atcgctggtg gaataattca gcgtaaaaga agaagaggat ggattcggga
     6481 aaagtcccgt gatggaagct cccccggcct cgctgatgga agaggtcagg tattccgcat
     6541 cgaacatcgc cacaccattc gatcctgcac cttccgcaat ccacgcatgc gcggtttgcg
     6601 tgagcatggg attgatctcc acacgcggcc aagccgccct gaggttaccg gtcatgtcgt
     6661 tgtattgcgg agtgatgtag ttccacgtat taggggcgac aagattattt ccattcacac
     6721 aatacggaag ttttccatcc gtggaatcat aataaacggt gagaaaatta tcatagcccg
     6781 gatcatagct gatgtccggc tgaagatcat tttcccctga gttgacgatg tcgaggcggt
     6841 tccagaagtt agtgtagtga gcccgtttat tgtaaaagcc cagcaggtca taatcggtac
     6901 cgttcccgta aaaatcgcgc tggcaaagaa cgaccgctgt aactgaagag ctgtcgttat
     6961 ccgtgccgtt gaattgaacg gcgatctcag gattgcagca gaggttgatc atggaagaag
     7021 aaattgaatc gaggttgacc ggcggtatcc aggcgtcatt caccgtagac gtgctccgcg
     7081 aggtgtagat atgacctgtg cggtcaccgg aagacgtcag catttcccat gccccgaaat
     7141 agcggccgtt ggatgcgctc gcactgcgcc cgtaggcaag cgaaacattc cggaaataat
     7201 acccggttgt catcacggac tgacgcacac tccatgttcc gccgccgtca actgacccga
     7261 gaaacaccat cgaatcgacc gagctgctgt aagcagaata aagaagcccg acgctgtacg
     7321 ggctcgcacc gaccgccgga aagcggtaat cggaagcgag agcaacgtca tatattctcc
     7381 gcgttccgtt ctgcaggttg taattgctgc cgatgaagct gccggttgtt gcattgtaac
     7441 ggtcaacaaa cagaacatag ttcccggacg tcgtattatg attcactccc gcgagataca
     7501 gcgtaagatt attcgtatcc gttccgcaga cgacgatgtc gaacgcagga taaacgatgt
     7561 tctgcggact atagctgtcg acggtcgtcc aggtcatccc gttgtcgcgt gaactgcgga
     7621 tggtaattcc gccggcaact ccggccgagt catacgtgga ataagccgca tacacccagc
     7681 cgttgaatgc aacgctgatg cggaccctgc gctgatcgat cgccggcgcg ttctggataa
     7741 taatgtcgtt cccgtactga accgtaggtg ccgaaggtgt gcgcgcgtcg ttgtgcgttt
     7801 cgctttgaga agctctgata gtctgcaaaa aacctcccgc cggtatgggc tggcggcccg
     7861 tctgcgcgga aagcatggta catgctccca gcatggcgat aaacgataag taaattgttt
     7921 tcatggctat attttttgtt acacatcaaa attagatcaa atagaaacgc agcagggaaa
     7981 aaatctccct gaaaaaattc cggcgacagg tgttgctttt cagaacactt tttcgcgcac
     8041 gagatcaaga attgtttttc cgggagcaag ccacaaggag gagatgcccg tttccgcggc
     8101 gcccttcacg tgctgcatcg aatcatcgat gaaaagtgtt tcggccggat cgagcttgtt
     8161 ttcatccagc acgcgaagaa aaatttccct gtccggtttg cgcatgccca tgcggcagga
     8221 gtaataccat ttttcgaaaa agcctgaaaa atcactgaat ccgtattcac gctgaagata
     8281 ggcggagaaa gccgcgacgt gaatttcgtt cgtgttgctg aggagaaaga gccggtagcg
     8341 tttcccgaga tgcgcaagca actcaagcct ctccggcgga acatccagca gcatcgcgtt
     8401 ccatgcctca tcgatcgcgc ggccggtgac cggcgcggga aaatattttt tcagctccgt
     8461 gcggaatgct tccggtgaaa ttttcccttt gtcgaaatca tcgaataatc ccgattgctt
     8521 tgccgtggaa tactgcgaag aaaaatcagt catgccgagg cgcacgaagg cctgttcagt
     8581 tgcggccgga tcaagattga tgatcacgcc gccgagatcg aaaatgatgt tgcgcagcat
     8641 ctgcgcaaag aactgaaatc cggttgtaac ctaaaaacgt atatttactt cacacgatga
     8701 aaggcaagcc cgtttatacg atttccggat tagtcctggc tgtcgcctcc gttcccttcg
     8761 tttttttcct ccacctgccg ctcgccgggg cgatcctcgc ggcgctcggg tatttcgtgg
     8821 cgatgaagga attttcaaag ttcaccgggc cgctgcagtt tttcacccag ctgtttgccg
     8881 ggctgatgac gggaaccgcg ctcgaatacc cgttccatgt atttccgttc ctgctgctga
     8941 cgatgttctt cgcgacgctg tcgacgttcg gaaggatcat gttcttccgg tttttcgcat
     9001 acacgggaaa aacctggttc gagccgctga tgcttttcct ggcgatcggc tgccacgtgg
     9061 ccggcaacat gtcggcgcac agcgactggg cggggtggtt gctcccggga cctgccataa
     9121 tttttgccgg gatcatcacg tgggggatca tcaaggacaa gcggcagctg ctggcgaaaa
     9181 cgatgaaagg atacaaaatt gcgatcggtt caccggcacc tcccttctcc ctcgaggcga
     9241 acgacggcac aacggtgagt ctttccgatt tttacggcga ccggaacctc ctgctcattt
     9301 tcgtacgcgg cgactggtgc ccgggatgtc acatgatgct gcgcacttac cagaaaaaca
     9361 gcgaacagtt ccggcagaaa aatatttttg ttctttctat cgggcccgac cctgtcggcg
     9421 tgaacaaaga gatggtggag cgcctgggtc ttgacttcaa agtgctggcg gatgaaggcc
     9481 agaaagtggc gatgaaatac ggcgtgcagc tgcaggaata cgacaatgca tttgccgaaa
     9541 aatatgacga aggaattccg cttcccgcat cgttccttgt agacaaaacc gggatcgtac
     9601 gttacgtgtc gagaccggat aaagtgggtg aatttcttaa tccttcgctc attttcccca
     9661 ttgtcgaaaa attggcgtaa tattcgttta tgtacttttt cctggccatt ttttccttcc
     9721 ttttctccgc aacatccgtt gtgctctgtg tgctgaaacc ggatctccag ccgtggatgt
     9781 tcccggttcc ggctttcctg ggatggatca ccgggctttt gcttatcaaa acaaggaccg
     9841 ccgccgtgga tatcgcggcc aacatttgcg gactcatttc gctgggattt tcactcgacc
     9901 aggcgggtgc ctcattcccg cttttcaccg tgacgcttgt cgtgctgatg ctcacgtact
     9961 cttcccgcac attgtttatc cggaacatcg gctatgtgaa gcagacgtgg atagagccgg
    10021 taacattact gacgggtatt gcgcttttcg ttttcgtgaa cgtcgtgaaa cacccgggat
    10081 ggtatggctg ggccttccct gcaccgatcg tgctgttcgg tatcgtatat tattcctcgg
    10141 cgcgcatcga gacaaaagca ttccggcgcg gcgcagcgaa cgagctgaaa gttggcatcg
    10201 gatcgaaagc acctgacttc gcgctgaacg acgagaacgg gaagagcatt tcgctcgcgg
    10261 agatcctgaa aacacagccg gtcctgctgc ttttcgtgcg cggcgactgg tgccccacct
    10321 gccacatcat gctgcgcacc taccagcgtg aaagcgatca cttcaaaaga aaaaacgtga
    10381 tggtccttgc catcggtccc gatccgcagg gcgtgaacaa agccatggtg gagaaaatga
    10441 gcctcagctt ccacgtgctt tcggatgacc ggcaggaagt cctttcgctt tacgggatcc
    10501 agttcctcga accgatcatg ggtcagaaat tcgaagtcgg atcaccgctt cccgcgtcca
    10561 ttctcatcga ccggaaaggc gtcatccggc acatttgccg cgccgaccgt gcgggcgagt
    10621 tcctgaaccc gctgcagata ttcaccgtgc ttgaaaaaat ggacacatga taaccgcggt
    10681 tattatttcg ggcattatcg cgctggcctg tgccgggttt tcagtgtatg cgcttctttc
    10741 gcggaacagg atgagatcgg cgttcgacgg gctgaacaag cagatcaaca attaccagag
    10801 catcatcgac caggcgaacg acgccatgat cgtgatcgac atcgtggacg gacgcattca
    10861 ccagtcgaac cccggcgccg cacagctgct gggttattca caggaagagc tttcgaaaaa
    10921 atcgcttttc gatctgcacc cgaaagaaga cctggaaaga tcctcgatga tcgtggcgga
    10981 tgtgtgggaa aaaggcggac ttatctacaa agacatcccg ttcgttacaa aatcaggaga
    11041 aattctcccg gtggaatgca gcgcgaaggt cgctcctttc gccggccgtc ccgctatcgt
    11101 gatctacgca cgggacatcc gcgaacgcct taagctcgaa ggcgagatcc agctgcagaa
    11161 aaacatcatc gagcagaaga acaaggatat cacggacagc atcaattatg cgaaacgcat
    11221 ccaggaagcc atcctgccca cggaagaaca gatgaaagaa gtggccccgg atcattttgt
    11281 tttttaccgt ccgaaagata tcgtcagcgg ggatttttac tgggcgacga gcgtaaccac
    11341 cacagatcct tcgcggcccg accagaaaaa atattccctc atcgccgcga tcgattgcac
    11401 cggccacggc gttcccgggg cattcatgag catcgtgggc cacaccatac tcgagcagac
    11461 gatcaccgac cccgacgtga acagcccggc ccaggcgctg gattttctca atcgcggggt
    11521 gatcaaaacg ctcaagcaga aagcgaacga tgatttttcc gtgaaggacg gaatggatat
    11581 ggccttctgc gccatcgacc cggaaaaacg gatcctcaat ttcgccgggg cgaacaatcc
    11641 cgtttatatc attcgcggcg ggcaactcat tgagctgtcc ggggacaaac agccgatcgg
    11701 cgcgtacatg aaagatgtcg ccccgttcac cgataaaagt ttccagctcg aaaaaggtga
    11761 ttgcatctac attttcacgg acggcatcgc cgaccagttc ggcggaccga agggaaaaaa
    11821 attcaaatac aaacaactgc agcaactgct ggtgaacatt cattcgcaga atatggacca
    11881 gcagaaaaaa accatcgtta aaacggtgga ggactggatg agcttcccca cgccatcggg
    11941 aggaacgttc gagcagacag acgacatgct tatcatcggc gtacgggttt cctgaatttt
    12001 ccataacgca tgaccagcga agagcgagca ggaatcgaaa aaatgcttgc cggcggagtg
    12061 agcggccggg agcgcgttga cctgctcaat aaagtggccg tggaataccg gttttcagag
    12121 ccgcagcgtt tctgcgatta tgcgacggaa gcctgcacag ctgcggaaga gatcgggtac
    12181 acggaaggtc ttgcacgtgc agaacggcat ctcggctatt tctacgagca acaggccgat
    12241 tacccgaaaa cactttccta ttgcagcaaa gcgctcgaac attacaagtc cgtgcccggc
    12301 ttatcgacgg agatcatcgc aacaaacaac ctggttgcga aagtttatgc caacctcggc
    12361 gattttacca aagggctgga atacctctac gaaagtctac ggctgtcgga agaaacggga
    12421 aacaaaacgg cggaaaccaa cacgctgaat tcgatgagcg ttctttacca gcgtctgggg
    12481 aatgcagcaa aagcggagga atgcgcttcg cgcggcctga cgctggcggc agagctaacc
    12541 gaccagcgtg tgcttggcgt gacgatggtg aatctcggca atgcgtacgg catgaccaaa
    12601 gactggacgc gcgcgctcga acagtggaaa acgagtcttt ctatttttga aaagctcggc
    12661 gacgaagacc tgcaatcgag cgtgctcggg aacatgggca ttacgcacat gaacctcggc
    12721 gaatacgatc gcgctgatga atacctctcg cgctgccttg ccatcaaaaa acaaacgggc
    12781 aaccggtacg acgtggcacg ttcgctgcag aacctcgctt ccgtcagcag ctcgatgaag
    12841 caataccgga aatcgctcga ttattgccgc gaaggtcttg agctcgcggc gctggttgat
    12901 gcacgttcgc tcgaatacca gctgtggtat gaaatggcgg ccgcgtataa aggtctcggc
    12961 gattttgaga atgcgctgac gtgctttgaa aaataccatt cgctggaaaa agaaattttc
    13021 acggaagaga tgcgcctgaa aacggatgcg ctccagctgc gtttcgccat tgaaaaatca
    13081 gaaaaagaaa aagagatcta ccggctgaag aacatcgacc ttgccgaggc gaacgaaaag
    13141 atcacgatcc agaaagaagt aatcgaggaa aagaaccgcg acatcacggc cggcatacga
    13201 tatgcgcagc gcatccagga agcgttgctg ccgcaggaaa gcgcgttacg ccatggattc
    13261 agcgaagctt tcgtggcata ttttccacgc gacatcgtga gcggcgattt ttactggtcg
    13321 ctcatcagcg aggacgcggt tctttttgcc gcagccgact gcacgggaca cggtgttccg
    13381 ggcgcgctca tgagcgtgat gggcgcggcg ttcctttccg agatcgtcaa tgagcgcggc
    13441 gtcaccgatc ctgcggaagc gctgaacctg ctccgcctga aagtcatcac cgcgctgcag
    13501 ggaaaggaag aacgcgatgc caccgaaagc ggtttacgcg acgggatgga catcgtgctg
    13561 tgccggtacg aaccggcttc gcggctgctc cgttttgcct gtgcgaacaa cccgctgtgg
    13621 ctgctgcgca aaggaaacat tacagaattc ggggcggata aattccctgt cggactgcat
    13681 cacggcgcaa tgatgaattt caccacgcgg caggtcacgc tccagcccgg cgaccagctt
    13741 ttccttttca ccgatggttt tgcagaccag ttcggcggaa cgaaagaaaa aaaattcggg
    13801 taccgccgcc tgcgtgagct gttcgtttcc atagcgggtc ttgcggcggc ggaacaaaaa
    13861 gccgctcttg aaaaagcctt ccgcgaatgg aagggagaga acgaccaggt agatgatctg
    13921 ctggtgatcg gcgttaaagc ctgaacggga tcaggtgctg acgggagcag aattcccttt
    13981 gcgcaatgta agcaccgtca cctccggcca tattcccacg cgaccgggaa aagcaagaaa
    14041 tccgaaaccg cggttgacgt agatgtaacg tttcgcttca ttatacaatc cggcccagtg
    14101 cgggtaggca aactgtaccg ggctccattt gacgccggga atttccacac cgaattgcgc
    14161 accgtgcgtg tggccggaca gcgtcatgtg cacatgagtc gggtgctgcg aaacgatctc
    14221 gtcgaaatgg gaaggatcgt gcgacaataa tattttgaac gcattttcag gaacaccttc
    14281 gagcgctttg tcgaggtcgc cctcatgaag aaatcccctt ccccagtctt cgacgccgag
    14341 caggtaaagc tgctggccgt ccttttcgat caccgtgtgt tcgttgcgca gcagcctgaa
    14401 gccgagctcg ccgtgaattt cgcaaagacg gttgaaactt ttcaggcgtt cctgctcggg
    14461 ccaggtgagg tacattccgt aatcgtggtt gccgaggatg gaatattgtc cgtacggtgc
    14521 gcgcagctgt ttcacctcgt tcatccacgg gtccatttca gaggccatgt tgttgacaag
    14581 atcgccggta aaaacgaaga gatcggattt ttccgcattg gccattgcga tcccgcggcg
    14641 cacttcgtca cggtcggaat cggggtcgaa gctgccgatg tgaatatccg aaagctgcgt
    14701 gatggtgaac ccgtcgaacg catccggcag atcgggaaag aaaacttctt cgcggcgcac
    14761 gcggaaacgg aattttccgg cggtaagtcc gtgaatgatc ccggcaaacg ggatggccgc
    14821 agtcgccgcc gccacggtgc tgatgaattt cctgcggctg cttccgaact ccatcgggtc
    14881 gttttttgtc agcacattat gcccgagcga ataaaatccc cgcaggagcc ggtaaacatc
    14941 ttctcccagc aggaaaagcg aaaacaccag cttgggaata aagaacagca cccaggcccc
    15001 gacgaacatc atgaacgaac gcggaaatcc tttgctgaaa gaagtgaaca gcaccagcgc
    15061 gaacatccac aggtaaagac ctgcattgat gctccagaaa gtccactgga cgattttttt
    15121 cgttcgtgat gaatatccgc tggtgatcgc ttttacgcct gaaaaaacgt agaaatcgat
    15181 gagcagaaga aagacaacga aaatgaggat gccgataaaa ccaggtgctc tgtgcattgt
    15241 gaaaacaaag gtactggtta gtggtcggaa tgattggtta atttatttta aacgggagaa
    15301 aatatcgccg ggaaaagatt tatctgtgcc tgcatcagct caatgaatgc gcctgtttgc
    15361 ggtttttgag atatgctcta cctttcgctt cccgtggaat gaaacacctg ctctcctttt
    15421 ttctttcgct tgcactttct tcgcttgccg cgcaggaaac aggacaattc cgccactaca
    15481 ccacggaaga tggtctttcg cagaattccg tttactccat tgtgcaggac cgccagggat
    15541 ttatgtggat ggccacacag gacggcctca gccgtttcga cggttatcgc ttcgaggttt
    15601 ttcatccgga tgctgccgat tccaattcac tttcgaacaa cagcgtgcgc tgcctccttg
    15661 tcgcacgcga cggactgatc tggatcggga cgcgggacgg cctgaacgtt ttcgacccgg
    15721 tgaaaaaaag gttctcgcat tttttcaaag cgaagaacgg attgatctcg ggaaatattc
    15781 tcagtcttgc gcaggacagc tcaggcaaca tctgggtgtc ttcgcttcat gaaggcgtca
    15841 gtgtctacaa ccagcagaaa aaaacattct cgtatttccg ccacgacgac agtaacccga
    15901 attccctctg cgggaacgac gtacgcggca tgtgctgcga tcataacggg cacatgtggc
    15961 tcgtcacctg gtcgaacggc atgagcgaat acgacccttc ttccggaaaa tggacaacct
    16021 tttccagcgg gggcggaccg ggacagcttt cgcatcccaa cagccgtggc gccatctgcg
    16081 ccgacagcaa aggaaggatc tggatcggca cgtggggccg cgggctcgac atgtgggatc
    16141 cgtcgaagca cgaaatgttt tccgaagacc gcaacacgcc ggaacttccg cagatgcgct
    16201 cgggtcttgt gtggtcggtt tatgaaacaa ggaacgggca catctggttc acgacagcgg
    16261 aaaaaggcgt ttatgatttc gatcccgcca ccggccatta caggaatttc atccacgatg
    16321 cagacgatcc tcgctccatc aacgacaaca gcatctggtc gatcaccgaa gaccggagcg
    16381 ggctcatctg gacaggcgcc tggcagggcg gcgtgaacgt gtacgatccg cgcatggaac
    16441 gtttcgcgct gttccgcgta aacccgaacg acagcaacag tcttcccgcg aatgtgacgt
    16501 ggaccttctg cacggacggc gccggcggaa tatggatggg aacagcggca ggaccggttc
    16561 attacgaccc ggtgacgaaa cagttccggc agcctccgct cgacgatccg gatcacaaag
    16621 tgctttacaa aaacgctcct acgccgcgct cgaacataca ggcgctttgc cgcgacggaa
    16681 acggcaacat ctggatggga acagccgggg caggattgca ctgctatgat cccgttacga
    16741 aaacctatga cctttacatg cccgaagaaa acaacgatgc ttcgctcacc ggcaatacgg
    16801 ttaactcgct tacgacagat gaagagggaa acgtctgggt ttgctgcggc agcagtcctt
    16861 tccagcgttt cgacccggtc agaaaagcgt ttgaaaattt tccttttgca aaaggagatt
    16921 cgatcaggcc caacgacgga ccggtctgca gcatctccgg cggacctgga aaaatgatcg
    16981 tcggtctcac aggcggagaa gttttcgagc tcgatgtaaa aacgcggaag tatacgctca
    17041 tctggaaatc tccttccgat gtttccgtta cgagtctctg cagggacaag cttggcagcc
    17101 tgtggatcgg catgggcgga tcgggactga tgttcatcaa cggaaaaacg cagacgctgt
    17161 ttaccgataa agatggcttg ccgaatgatt tcatcaacgg aattattccc ggggataacg
    17221 gcgaattatg gctaagcacg aaccgcggga tctgccgctt tgatcctgtg aagcatgacg
    17281 tacgcatttt tacgacggaa gacgggctgc aggcgaacga attcaaccag ggcggcgcct
    17341 tccgttcgca cgacggaagg atctggtttg gcggcgtgaa cggcatctcg gcatttttcc
    17401 ccgaccagat cacaccgaac acctctcccg ccgcggcgat catcacatcg ctgaccgtgc
    17461 ttaacaaaaa aatcccgctc gacagcgaca tcacgttcac gaaagagatc cgtctttcct
    17521 ggcgcgatta ttttttctcg ttcgagttca gcggacttga attcaccaac cctgagcgta
    17581 accagtataa atacatgatg gaaggtttcg atgacgactg ggtggatgcc ggcacgcgga
    17641 gattcgccac ctatacgaat ctcgatcccg gcgaatacac cttccgcgtt aaagcgtcga
    17701 acaacgacgg caactgggac ggccgcgaag cgcgcattca catcatcata acaccgccct
    17761 tctggaaaac aaaatggttc tatacgctct gcggaatttt catgctgctg tctgtcgtcg
    17821 gttacatccg ctggcgcgaa cggaaactga gaaaagagaa agaggtgctc gaatcgaaag
    17881 tgacggaacg cacgtacgag ctgcagcagg aaaaagaaaa agtaaccgcc gcgcacaaag
    17941 acatccgcga cagcatcaac tacgcgcagc gcatacagta tgcgcttctc tcgcacgacg
    18001 agctgctgaa gaaaaacctg ccggagcatt ttattttctt ccgcccgaaa gacgttgtca
    18061 gcggcgattt ttactgggcc acgcagcagg gacccgtttt ctggctcgcc gtctgcgaca
    18121 gcacggggca cggtgttccc ggcgcattca tgagcctgct gaatatttcc tacctcaacg
    18181 aagccatcat tgaaaagaaa atgacagagc ccggcgccgt tttcgattat gtacggagcc
    18241 gccttattga aaatatttca caggatggcg gcaaggatgg aatggacgcg atcctgctga
    18301 agatcgacca tgaaaaaaac acgatcacgt acgccgcagc gcacaatgcc ccggttgtaa
    18361 tcgtcaatgg aaatacgctg gtgacggaaa ctgataaaat gccggtaggc aaaggagagc
    18421 gaaccgatca cttcaaaaca cgaacgctgg aaaccgttcc cggtgaaatg atctaccttt
    18481 ttacggacgg ctgtgcagac cagttcggcg gaccggcggg gaaaaaattc aaagaggtca
    18541 acctgcgaaa aaatctcgcg gaaatttctg cgcttccggc gggcgaacag cgcagccggc
    18601 tggaaaaaac gttcgaagac tggaaaagcc atcatgaaca ggtagacgac gtgctggtga
    18661 tcggtgtgcg aatctgacga agtccaacat cccgcaacca gccgcctgca gagcacaccg
    18721 ccaaaaccaa ttgccgtgcc ggcgggcaaa cgtgggcaaa cccggttccg gtctaatttc
    18781 tatttttgca cctcgtttgt tgcgctatgc gcaacgggcc catagctcag ttggttagag
    18841 cagcggactc ataatccgtt ggtcgcgggt tcaagtccct ctgggcccac aatgcgtcag
    18901 tttccaagaa gctggcgcat tttcatttgg gtggtttgcc gatcaggaag gcgacttgtg
    18961 gcggctttgc cgcgcatctc tcgctacgaa tgtccaccgg acattcgctt aacgcgcgtt
    19021 ctccctctgg gcccacagga taaacgtaac gtagtcgccc gattgcgtgg cgtttgggtt
    19081 tccgggcgaa aaggagtgcc ggtaagtgca gtgtaagagc ggatgcacac gaaagagaaa
    19141 cgatcttgaa ggagttgaga aatgtatccc ggaaaataaa gtgcttactg tcgccgggtg
    19201 atactatgtt tctttctata aatactgttt gagtttcgcg cctcaggtat atacggtcct
    19261 caatcctcaa ctgcattttt ggtcaaattc atcacggagg aaatacccat tacgagcgcg
    19321 gcggccctca gccggttttt ttgtgtgttg aatttcattg cgcgggaatt agttgtatga
    19381 atcgccggaa ttcctttaca tgcattgcca tcgctgcctg aagcctgtgc acccgtttca
    19441 gctctgccgg ttcccggaat gtttttaact gcgaaaggtc cttctgcagg aaaaaattac
    19501 ctgctgccgg gaacctcttt tcccggcaag agtccgaacc ctgctttcac ccgcataaaa
    19561 agactgcgta atggttccag tatctttttc gccgccgtct gcagaaatcg cttccggttt
    19621 ttccccgggg cgccgctggt ttccttgtcc attgccagcc ggtaaatgcc gtttatgaat
    19681 tgcccggaag caagataatc cgctacggac cggttcctgt ccgaaataac ggactgcttt
    19741 ttcccggagg gaccattgac ggttttcacc tgaagattgt tctcatagaa attcctgtac
    19801 ggcctgtagg gccgtatgtt cggcgagtag gtttgcataa ccgctttgta tggttttatg
    19861 ccggccgtgt aattcattgc cggcatgcgc ccgttatttg cagctgcaaa ggttttttgt
    19921 tcataatttg catccggaat tttttaaaaa atacaaaaat tcatcactgc ctgtcatgaa
    19981 atcgtaaaat attccggtcc ctgcaaatct tcctgttatt tatcattatc attacatcaa
    20041 taaccggggt gtaatgacct gattcaatga tccctgatca caggttgtgt tatccgtccg
    20101 taaaattatc cgcctgcatc gcatttttga aaatatgttt ccggtaatcg attattggtt
    20161 tactttagta atataataat cgcattattt aaagtattaa ataaaaaaac actaatcaaa
    20221 cccaacttaa aaaaagattt atggcagaaa ctgctttagt atcgatggcc cagcaattca
    20281 ccgggctgcc gatggatgcg ctgatcggcg ctcctttgaa tgccgcggcg aacgcaaacg
    20341 ccgcaatggc gatcacacag acaaagttca tgctcgacac ctgcttttca cgccagggtg
    20401 acgacaaaac cgggtacaac tacacgccga tcatgatcaa gatgtcgctt acgcgcggcg
    20461 tgatcacacc tggagacccc aacgccaacc cgcccacaaa gacggatatc cagagcgtga
    20521 cgacgacatt cgacctcccg ctgctgacca ttattccgct gaactcgctt ggcgtggata
    20581 atgtcaatgt cacgttcgaa atggaagtta aatccagctt cgccgaagaa caatccgaag
    20641 cgacggaaaa aaacatccag gccgaaacca gcttcgaggt gaaagtgggc tggggcccgg
    20701 tttctgccac ggtgaaaggc agcgccagct acgaccagaa agattcttcg aatcacagca
    20761 cacactacga aaaaagcaac tcggcgaaat acacggtact cgttcatgcc ggccagcttc
    20821 cactgccgaa aggcgtgaac accatcatcg aagcctatac aatggctatt cagcccgtca
    20881 cgatgcccgt tgtgacgccg cccaaatcct gaccaggccg gtaaggcagc cggcgcataa
    20941 acccgccggt tgcttttatt ttgtgcctgt ttctggagca ggtttttccg agggcatcta
    21001 tcctgtaaca ttcattgcct atggcaaatt ttcaaacgaa aacgctggac ccttccgact
    21061 attcgggatc cgacatcgcg gcgatcatcg gggctccact cgtggcggcc gcacatgcaa
    21121 atgcgatgat ggcccgggag cagatcaagt tcctgatgtc tttctgcttt tcgaaaaccg
    21181 gtgaagtgta cgagccggtg atgatccgca tgtccatgac caaaggcgtg cttactcctt
    21241 ccgacgaaaa agacaaacct ccgttcatcc agcagctgac aacgacgttt gaaattcccc
    21301 tgcttacggt cattcccatc aactcccttg ccgtgcaaag catggaagtt aattttgacc
    21361 tcgaagtgac ttcacatatc aacgtgccct cttcgcgaaa ttcccccggg aacctgaaac
    21421 cggatcataa atccgttcgc atgatgggaa agatcagcag ctctcccggt gacggacagg
    21481 gtacggataa agactattac gacaaaacgg gcgcatcgac attgaaggtg aacatcaaat
    21541 cgggaccgct gccgttgcct aaagggctcg cttcggtgct cgaggtttat tccaagaaca
    21601 ttcatcccgt agaactcaat cctggcgccg atactgcaca aaccgacaaa aaataacatt
    21661 ccccggtaat ctgccggccg gaacagcgtc tattcatccg acaaaaaaac aacatgagcg
    21721 atcaactcag cattccctgc cccgactgta aaacacccat cccgttcgac ccccggatgc
    21781 ttatccaggg cgcgcagttc tcctgtccca aatgcaaagc atttctgggc ctcgcatcag
    21841 agagcaagga aattgtacgc gagtcgctgg ataaattcga caagatgaag agcgaagtgc
    21901 tgaaagccaa aggagacacg accgggtttt aactgctatt gttatgataa gtttcaaagc
    21961 ttttatcgaa ggtatcaaca gtgcgatcgt cgctgcgaat gacgacctga tgaacaagca
    22021 cgaggagatt cttgatacgt atttcgaaaa aacaggagag gacaaaacgg acggacacga
    22081 aggtcattcc acgctgcggc cgaaatccgt ctgcattcaa tatcccaaaa tggaggttga
    22141 cggctccatt accatgatcg acgtggacgt tccgctcgtc acgctcattc ccatcgagtt
    22201 caccaagatc gacaaagtca aaataaccgc acagttcgga ttgcaggtaa taaataatga
    22261 cctgcagatc attttttcca atgaggcgcc caaaaagaaa tggagcctgt tcggcggatc
    22321 aaaaacggat gacaaaacgc cctcgccggg acagcttgaa ataacgatcc gcccttccga
    22381 aacgcccgaa ggactcaaaa aaatgatcga cgggtacgat aaagtgctgc gcgcacagat
    22441 cccgcactga tcttttccgc aacaggtttt tttctcatta aaccgcaaaa cgatgccttc
    22501 attatccggg ttccccgccg caaaacagga agatcccaat tcctgctggg cctgcgcttc
    22561 gcgcgaaata agcaactatt acaagtcgct cggaaaaggc ggaaaaaacg aaatgtattc
    22621 ttccgacaag gaactcgccg cagcgtggtc gaagatcacg ggtaaaccgg gcaacgcgga
    22681 tatcagcatc cagcaatcgg catcagccgc cctcggggat ctcggctata cgaattccgc
    22741 tgacagcgcc ccggttccaa cggtcggtga gatcacggac gcgatcaacg acaagaaacc
    22801 cctgcttgcc attgtcggaa gcaccgatcc gaaaggtaaa cgcaagctcg gttaccagca
    22861 gggtcattgg gtcgtaatca cgggcatttc cgacaaaggc gatctcatcg aagtcttcga
    22921 tcccgacagt ggaaaaatcg aaaccgtagc ctacagcagc caggtctaca aaagcggcat
    22981 gttctggcaa aacacctcgt acgttgacgg ccagtaaatg ccggaagatg atctcaccac
    23041 gccactttct gcgtgatccg gttttcttca tcaccgaaca cgaccaggaa catatcgtgt
    23101 gaaggaacgg caagcggaat aacattgtcg ccgtttcccg aaacggtttc gtatacgagg
    23161 cgcccggaag aatcgaaaat ggaaatatgc atttcactgc tccagttcgc agaacgatag
    23221 tggatccctg ttccgtcata ccacgcgttt gcatatacgc ggtctgtttc tgccgtgacg
    23281 gaaatgatcg cacttacgat tgacgcgccg ttgaaatcgg tccaccgcag gcggtaataa
    23341 ttctgcccgg gaagcggcgt cacatcggtg aactcatatt caaccggctg gctcgtggtt
    23401 ccgcttccgt tcaccgtccc gatcgctgtg aaatttccgt cggcgcctgc acgttccacc
    23461 gtgaagaacg aattgttgtt ctccgattct gttgtccagc tgagcacaac gcttcctccc
    23521 tgcacctcgc cggaaaatac ggacagctcg atgggaagca ccgtgaacgt ggtgcagccg
    23581 gtattgatat acgttgcgct gtgcccggcc gaaccgaaat acgccatttc cccgttgttg
    23641 ttcgggatcg ctccgcgatt gtaataattc gtatcgacgc agccggtggc gttgttgcgc
    23701 aacatggcat cgcgcgggca cgcctggccc gtgcaggccc cgttgttccc gaatcgtccg
    23761 ttgttatcgc tcgtgttgtt tgcaaaaacg gcgtagtacg tcaatccggt gctgcacagg
    23821 ttactgaagt tgtacgtata ggtcggcggc gctcccgcaa acacaatgaa gtttccaccg
    23881 gcagggatca catttcccgg cggcgccatg aaaagagttc cacaaccggc catggtattc
    23941 aggctggcta tgtatgtgtt cgtcgcagca ttgttcacga aaaaattatc gcagggattt
    24001 ccggtcgttc cgcaatacgt gcatcctccg ttcgagccta catcggaaaa tgtgatctcg
    24061 agatcggaaa ggttcatcgc aggtcctccg ttggcgcagg aaacaaattc gtcgtaacct
    24121 tcatccgatc cgcatgcatc ggccatgatg cccgtgatag aaagctggcc cgagcaaaat
    24181 cctgctgcac gcactttgac atcgtcgaaa gcgcagttcg ccaccacttt tgtatacgtg
    24241 aaacgaagac gggtgacgtt cgatgccagc gggaatacaa aaactttcgc tgtcgtctgt
    24301 ggataagcgc ggcccaccgt cgtccatgaa gaaccgttcc agccttcgat gagaaaattg
    24361 tcggtcgtgg gtgtagatcc gttcccgcgc gtgaagaatg tcacttcatc cgcgcttccc
    24421 gaccatgcgg ggctcagcgc aatttcaccg cttccgtcga atttgacgga tggggatgac
    24481 cggccataat tcgccgaaga tgtataggtg gaagagatgg atgtgaatgt ccatccggca
    24541 ggagctgcag gagccgcgtt aaactcttca tcgattagga atgcctgggc gttcgctttg
    24601 acacagaaaa tagtcagcgc gagaagcgca aagaaaaata tacgcgtgcc tttcatggct
    24661 gggtcgggaa aatgtatgag gaatttatcc ggcaggtggt ttcggctgat ccgggaaagc
    24721 ggatgcttca agggcattca cctgtattga atttcgccaa aggtattccg ggccccgaaa
    24781 ctttctgtta gcgctgtgtg atagttgcgt gaacggaaga aaaagaagtt acggtccgtt
    24841 tacatcgatg tgctgacagc cttgtgccgc ttccgcatcg cggtgaaaac gatacggggc
    24901 gccggtgctt aatgcgcagg tcatgcggga gagaataccc gccagttgct ttccgccgcc
    24961 gaacgacaga atgcccgggt ggcaggaaca taaagcgctg catcacgaaa caagcagaac
    25021 agcgaagaga aaaagggctt cacaaacaat agctacaaaa ggcggatcac attccggtct
    25081 atggtttctg cgaaatgggc atgctccagc tgctgcaccc gcgcagacaa cgtctccggt
    25141 gtgtcgccgg gaagcacggg acacgtaaac tgcgcgatgt gctttccgtc gtcgaatttt
    25201 tcattcacga aatgaatagt aaccccggtt actttttctc ctgaagcgag cacggcttca
    25261 tgcacttttt tgccgtacat ccctttgccg ccgaatttcg ggagaagcgc cgggtgaatg
    25321 ttgacgatct ttccggggaa tgcagaaatg accgcgggcg gtatcagcca gagaaacccg
    25381 gcaaggacaa taagatcgat gccgcgctct ttcagctgcg gaacaatgga atgttccgaa
    25441 aaaaattgtt cgcggtcggt aagcagcact tccactccgt gctccttcgc gatggaaata
    25501 acgcccgcct gcgggttgtt gcatacgagc agcgttacct gtgcgttgtg atgaggttcg
    25561 aaatactcaa tgaggttccg ggcattggtg cccgttcctg aagcgaagac cgcaattctt
    25621 ttcacggcgc aaaaatacaa ataggaacgg cgctcagttg aaatcgaatg tgccggtgct
    25681 gtcgcgggaa atttcccttg aaaaaatcat gcctgccgga tcgatccggc ctgtgctcac
    25741 ggttttcacc acgccgtaaa cgggtttgtc ttccccctca tcgcccatcg tgtcggtgct
    25801 gcacgagcgg aacgttccgt cggtgtatac attggttcgc tgctgcgaat gaaaaaacac
    25861 aggcgcatcg gcgccggaaa tatcctgcgc gatcatgtac gaacggatgg cctttccgtc
    25921 tttcccgtac gtagtgatca tgaaataatc gccgctgctg aacgggcagg cttcatagga
    25981 aacgaaatgc atgcgccagg caaccgcgca gaaaggacca gcgtttttga gcgtgtcgag
    26041 cacgtcgatg tccacctgca tgatgtcgcc cacatcgcct tcgtattctt ttcccgtttt
    26101 gcggatcgag tcgagctcga aataacgcgc gaacgtaaac agcgcggagg gatcggctgt
    26161 tttgtcgagc ggccagaatc ttttcctcac ttcgcccgct ttcagcagaa ccggtttccg
    26221 cccggataat ttttcatcga accggaaaag cacgagcgag ggatcacgcg gctcgagcgt
    26281 atcgccgtac agcgattctg caacgggctg cctgttttcc gtttttgcga cggaatcttt
    26341 ttttgcttca ccggaaacgg tttccttttt tacatcgttt ccgcacgaaa aaagaaacgc
    26401 gccaaaacat gcgcaggtca gccactgaag ttttttcata tacgcaaatg tgcaaacatt
    26461 ctgccgcaga acacggcacg aaaaaaaccg tgagctcaca cggaaattat tttttgtacc
    26521 gtatcctgtt cgggttcatg gccaggtaac gccgcatggc gccgaaatcg ccgaaggtca
    26581 gctgcagctc gctgccgttc acatcgaggg aaaccgcatc ctggtgatcg gcgcagtacg
    26641 ggatgttgct taccgatccg gaagcgacgg aaagatgacc ggcaagcagc ctgcccacgc
    26701 tgacgctcgt ttttttcgcc tgcgcatatt tcaccaccgg tgatccgcag acgatgcatt
    26761 catccggcca caagctggtc acgaaaaccg ggcaggtgaa tttgcttttc gcctgtgtgt
    26821 cgttttccgt gaaggcgcgc agcacgcctt tgtcgctgat gagccattcg cagcagcgcg
    26881 gacaggctac ctgcagatcc gggtccttca tgctgatcgt gacgtccgtc gtgctgccca
    26941 catcgaacgc acaatacggg catttcgcat ggcgcgaccg caatgccgcg atcgtggtca
    27001 gcaccagcat caacgcaccg ataccgatgc ccgtgtacat tcccggcgcg cccgcgatgc
    27061 cggaaccaat cgccgaagca attccgccga cgatcgccga agccacccag aaggcgaata
    27121 tttttttcga tttcgttgcg aacgtagccg gaacaagcgg tgatacaacc ggcgaaaaag
    27181 ccggcctgac acgaccgtga tacgccgaca ctgctgactg ttcctccacc ggtttatgct
    27241 taggggtaat aaaatctaag gtgaactgtg aaaataaaaa aattgcgcca cggggaaaat
    27301 ggaaaacttc ccgggctaaa aattaccttc accggtaaat catgttccca tgaattttcc
    27361 tttcatcccc tatacgcacg aacgttttcc ggatgaagaa atgatcgcga gaagccagtc
    27421 tttttccgaa tggatggata aacgacgaac ggtgcgcgat ttttcttccg aaccatttcc
    27481 ttttgaagtg ctggaaaata ttgtccgcac cgcttccaca gcgccctcgg gtgcacacaa
    27541 gcagccgtgg acattctgca tagtgaccaa ttcagaactg aagaaaaaga tccgtgaagc
    27601 cgccgagaag gaagaatacg aaagctacaa cggccgcatg acgcccgaat ggcttgaaga
    27661 tctggccccg ctcggtaccg actggcgcaa gccctttctc gaaacagccc cggcgctcat
    27721 cgtggttttc cgccggccgt atgaaattgt caatggcgtg aaaaagaata attattacgt
    27781 gaacgagtcc gtcgggcttg cctgcggatt cctgctggcg gccatccacc atgccggact
    27841 tgttgcgctc acgcatacgc ccagcccgat gaacttcctg gcgcaattgc ttgagcggcc
    27901 ggaaaacgag aaaccgttcc tgcttatccc ggtcggacac cccgcgggaa actgcacggt
    27961 cccggatatt cgccgcaaac cgcttgaaaa tgtgttagtt gcctataaat gagcctttta
    28021 gattaatgtt tttgttattt accccatccc tctatctttg caaccgtctt ttaaccaaaa
    28081 aactgcttta aaaatgtctg acatctcagc aaaagtgaaa gctatcatcg ttgacaagct
    28141 cggcgttgac gagaaggaag taacggaaac agcgagcttt accaatgatc ttggcgccga
    28201 ctcactcgac accgttgagc tcatcatgga atttgaaaaa gagttcaaca tcgctattcc
    28261 cgatgaccag gccgaaaaaa tcaacaccgt cggcgaagcc atcaagtaca tccaggaaca
    28321 cgcgaagtaa ttccattctt ttctaaaaat ttcggggcat gcaactgaag cgggttgttg
    28381 tcacaggact tggagcaatt acgccccttg gaaattctgt gcaggattat tggagtaacc
    28441 tcgtcgctgg caaaagcggc gcggctacca ttacccgttt cgatgcgtcc aaattcaaaa
    28501 cgcagttcgc atgcgaggtc aaaggcttca aaacggaaga ctacttcgac cgcaaagaag
    28561 cccggaagct cgatcctttt tcacattacg gcattgcggc cgccgaccag gctgtccgcg
    28621 atgccggtgt gaatgccgat tccattgaca aaacccgcgt gggcgtcatc tggggatccg
    28681 gcattggcgg acttcagaca ttccaggacg aatgcttcgg gtttgccaaa ggcgacggaa
    28741 cgccgcggtt caacccgttc ttcatcccga aaatgatcgc cgatatctgc tcgggccata
    28801 tctcgatgcg tttcggcttt cacggtccca atttcaccac cgtttctgcc tgcgcctctt
    28861 ccaccaatgc actcatcgat tcattcaatt acatccgcct cggcaaagcg aatgtgatcg
    28921 tcaccggcgg atccgaagct gcgatctgcg aagcgggaat cggcggattc aatgctgcgc
    28981 aggccatgag cacgcgcaac gattctccgg aaaccgcttc acgtccgtac gacaaagacc
    29041 gcgacgggtt cgtcctcggc gaaggcggcg ctgcgctcgt cttcgaagag ctggagcacg
    29101 cgctgaaccg cggggcgaaa atttactgcg aggtggctgg cggcgggctc agcgcagacg
    29161 cctaccacat cacggcgccg catccggaag ggctcggcgc tgcactcgtt atgcgcaatg
    29221 cgctcgaaga tgcaggaatc acggccgcag atatcgatta catcaatacg cacggaacgt
    29281 caacgccgct cggtgatgtc gccgaactga aagcgatcca gaaaattttc ggcgagcacg
    29341 cttataagct caacatcagc gccacaaaat ccatgaccgg ccaccttctc ggcgcggccg
    29401 gtgccgtgga ggccatggcg acgatccttt ccgtcgtcaa taacaccatt cctcccacga
    29461 tcaatcactt cactgacgat cccgagatcg atccgaagct caatcttacg ttcggcaaag
    29521 cgcagcagcg cgaagtgcgt gcggcgatca gcaacacatt cggtttcggc ggacacaacg
    29581 ccgcggttgt cgtacgcaaa ttttccgcct aactttagcc gtgttttcag cgttcagagc
    29641 ccttttttct tccgggccgg ataaggcttt ccggaaaacg ctccgaaatc ttctcggctt
    29701 ctggccggat aatatccgcc tgtaccggaa agctttcacg cacagctctg cggccaatct
    29761 tgcgaaagac agagagcagc cgcgcgaaag ctatgaacgg ctcgaatttc tcggcgacgc
    29821 cattctcagc gccgtgatcg ccgattacct cttcaagaaa tttccctacc gcgacgaagg
    29881 cttccttacc aagctccgct cgcgcatcgt cagccggcag acgctcggaa agctcgcggt
    29941 gaaattcggc atcgacaaat tcgtgcaggc ggaatccggt cttgcgggaa gaagcaattc
    30001 catcaacggc gatgtctttg aagcgctgat cggcgccatt tacctcgaca agggctatga
    30061 tttcacctcg gattatatca atgaacgaat ccttggtttt catcttgaca tagacgaaat
    30121 agaggcgacc gacaccgatt tcaaaagcaa gctcatcgaa tgggcgcaga agaacaggaa
    30181 ggaccttcgc ttcaatcttg caggggaagt cggtacgggg cagcacaagc aattcataat
    30241 cgaggttacg atcgacggcg tagtgcatgg ccgcagccag catctttcga aaaaacgtgc
    30301 ggagcaggaa gcagcaggat tgtgtctcgc ggaactcaag ctgtcctgac aaaaccggcg
    30361 gctgaacagt cttttttcag tcgaaaacaa gttcccgctc tttcagcaac cgttccggcg
    30421 tgattttttt atcacgtccc tttttcgttt tgttactatt cataaaggat gccttttccg
    30481 taattaaaca gtggagggaa agtcattttt tcaggcaggc ggcattgttt tctgctttct
    30541 tttcgacgaa tggttccgca ccacaatatg tgggacgtgt ctgcgttttg aggcgacgaa
    30601 catgagtttt ttacgcttat ttgatatttc acctatggca ttctgccatt ttggaagcaa
    30661 cctccgcaac cttttcacgt cttctaaccg taaaagtagc ggacaccttg ccgcgatatg
    30721 aacgatcatg aacttatagc cggttgcata aggcaggatc gcgcctgcca ggaagcgctt
    30781 tacaagcggt tttacggtaa aatgatgggg gtttgcctcc gttatgccaa aagccgggac
    30841 gaagcgcggg acatgctgca ggaaggtctc atcaaggtgt tcacatcgat ccggaacttc
    30901 cagggaaagg gttctttcga aggatggata agacggatag tcgtgaacac ggcggtggat
    30961 catctcaggc gcaacaagca tgaatacatg atcgtctcaa cgatctacgc gcgggaaggt
    31021 gatatccctg atcaagtgga agaaatggaa gaggacaatc tcatcgccaa cctttcggaa
    31081 gaagaaattc ttgcttccgt gcagcagctg agcccggcct accggacggt gttcaacctg
    31141 tacgtgatgg aaaatttctc gcaccgcgag attgccgacc agctcggcat cagcgaggga
    31201 acatccaaat ccaatctttc caaggcccgc taccagctca agaaaaattt aaccaacctg
    31261 atcaaaaaca ccgatggaga acggaccttt acaagatgac aagctcttcc gggctgtgca
    31321 cgatcggctg agcgactacg aggctccgta caacggagct gactgggacg ctatggaccg
    31381 ctcgctcgac gcacttccca aaaccaaccg ctttcagtgg aaattttcgc tgaatacgct
    31441 gctgatcgct gtcggcgtgg cctcgcttat ctcgctgggg gttttcctcg cttcgggaca
    31501 cagtgcggcg cctggcaagc cgcaggcggt tgcacaaccg gcaacagaca ataatcttcc
    31561 ttcgaagaat acgtccgttg taacgaacgc tccgccgtca caggtgcaaa ccgtgaacat
    31621 ggcggacgtg caggaggttt cttctcccgg tctcgtcacc gggaacagca gcaatccttc
    31681 ccccggcaac acggatgtag ttcaaaacac atcgtctgcc aggaaaacga aaaccaaaaa
    31741 agattccggc ctgcttttcg gcgaccagat cgatccggcc aaggggttca ttcaccggac
    31801 gcaggaagac cagaagctga tcgaccagta tgccaaccgc ggtacgccgt ctgtttattt
    31861 tgataaagag aacgggcagc tgaaagagat catcatcaac cgcgacagcg cacacgtcac
    31921 cggcatcaaa aaagattatg cgcctgccga cacaacggca aagaatacgc cccgcggagc
    31981 tcctgttgaa acggaacagc acgggtttga ttttaactga gtattcgtct cccgaatacg
    32041 acaacaggcc tccgggcctt tttttattcc tgtttttttc gttccgaaag caaccttttt
    32101 atttccgccc gtctttttta tggcaacacc aaaaacttac gggcatgaaa acactggtac
    32161 gaattttttc cgtcgcggtc ctgcttgctt ctgccgctga tgcgctgcac gcacaggatc
    32221 ctgaattcac acagttttac gcgaacccgc tttacctgaa ccccgctctc gccggttcga
    32281 aaatctgtcc ccgcttcaac ctttcctacc gtgcgcaatg gcccggcatt tacggaacct
    32341 attccaccat cggcgcttca ttcgataaat acgcctacaa agtaaaaggc ggcataggcg
    32401 tgacagtggt ggaagaccgc gcagcgaaag gaacgctcaa cacaaccggt gtcggtctta
    32461 tttacgcccc ggtcgttccg ctgggaaaag acatttccgt ttccgcagcg atacaggccg
    32521 gctactggca gaaaactgtg gactggtcga agctcacttt cggcgaccag atcgatccgc
    32581 attccgggtt taatcttttg acacaggaac cctcgccgca aacacagcgc gaaggtgatt
    32641 tcgatctcgg tgccgggctg cttttttctt cgcggaattt ttacgccggc ggcgccgttc
    32701 atcatatcct cgagcagaat gaatcgttcc tcggcggatc gagtccgctg ccgcggaaat
    32761 acacgctgca tgccggggga atgatcccgc tcacgcacga ccgttacgaa ggcgactcct
    32821 acatttcccc gaacattctt taccagaagc agggcgattt ccagcagctg aacatcggcc
    32881 tgtacgccaa gaaagatgcg atcgtcggcg ggctctggta ccgcggaaac gattccttca
    32941 tcatgctgat cgggatagaa gcgggacagc ttcacctcgg ctacagctac gacgtaactg
    33001 tttccaaact ttccaatgct tcagccggtt cgcacgaagt gacgatgggt taccagatgt
    33061 cgtgcaagcc gccgaagaaa aaatacaggc ccgtgaattg tcccggctgg taggcagcca
    33121 ttcccagaaa ccggtttccg gggttgtgaa aaatcagttt gcttccctgc caggaccggc
    33181 tccggaacct ggtttccggg aacgattttt atcaggtcag gtgtgcgtgc gccgcggttg
    33241 gcctcccagg cagaaatacc gcggcgcctt ttttgctttc ttcccggttc ttaatgaaat
    33301 cgtagattcg cgcatgcgca ttttcacacg cgaacgtctt atcgcactcg ggcttttctt
    33361 cgttttcctg ttcgggatga cgcagttttc ccggaaaatg cattacgccg tcgggatggg
    33421 aacctgcatc tggtcggacg gcatcggcta ctacgaatac cttcctgcgc tgttcatccg
    33481 ccacgacgtg aaccacatcg gctggggaat gcccctgccg aacgggaacc gtttcgacaa
    33541 atacgccacc ggcgtcgcca ttcttgaaac gccgttcttc ctcgccgcag acgccgcaac
    33601 cgtgctcaac caccgtgaac gcaccggatt ctcttcctat tacggttacg gcgtcctgtt
    33661 cgctgacctg ttctacacgt tcgccggttt tattttcctt ttttacacgc tgcggaaatg
    33721 gttcagcctg cggacagttt tcttctcgat cattctcatt gcgctgggga cgaatctttt
    33781 ttactacggt ttcgcggagc ccagcatgtc gcacgcgtac tcctttttcg ctttcgcggg
    33841 atttcttttt tactgccgca aattccatga agaaggacga agatcgcggc acctccacgc
    33901 ggtcggtttt ttcctcgggc ttgccaccgc cattcgcccg acgagcattg tcatcggcat
    33961 cttcttcctt ttgtatggcg tttattcggg gaaggatttc cgcgaacggc tgaaaacatt
    34021 tttctccgac cgccggatca ttcccgcatt gctgttctgg ggcgtgctgc tgttcctgcc
    34081 gcagatgctg tactggcatt ccaccacggg aaaatggttc acgtattcgt acggctatta
    34141 cgacgaaggt tttacctact ggaaatcgcc gcactttttc gacgtgctgt tcgggccgtg
    34201 gtgcagctgg atcccgtaca cgccggtgat gatctttttc tttcc
//
LOCUS       DASTSF010000200         2996 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_856605,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000200 DASTSF010000000
VERSION     DASTSF010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2996
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_856605"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1099)
                     /gene="atpB"
                     /locus_tag="VFU15_05885"
     CDS             complement(<1..1099)
                     /gene="atpB"
                     /locus_tag="VFU15_05885"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017259480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEU4717338.1"
                     /translation="MRSNALINRFSLFLAALLIPAFGFCYEMEDQASDSSASNVKVAD
                     AGAPKKEGFNPGEMIMEHVSDSHEWHIFGNIRIPLPVILKTDKGFECFSSGNFRNPQT
                     HELQPYQGKYYTYAYNKDGKIIVWDSVAMAQNQVASKSIFDISITKNVVSLFVIVGIM
                     FLVFLNVAKAYRRRPGQAPKGMQSLFEPLILFVRDDIARPSIGPKYERYMPFLLTVFF
                     FIWISNLLGMIPVFPGGANVTGNIAITMTLALMTFVITTFSANKNYWRHIFAMPGVPF
                     GVLFILTPIEIMGMFLKPLVLMIRLFANILAGHIIALSFFSLIFIFGKGGEALGAGLG
                     VSIGSVAFTVFMTVLDILVAFLQAYVFTMLSA"
     gene            complement(1296..1967)
                     /locus_tag="VFU15_05890"
     CDS             complement(1296..1967)
                     /locus_tag="VFU15_05890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717339.1"
                     /translation="MKKTLLIFSLALITFQVNTLSALTPQGKSDDVKSYWLGPGFGYY
                     FLPMASVNAQLGGYGVKTGFGNAMGVGLDYSAHVSNDRLPRGGSSLYSFHYLLPQTVK
                     AGDSLTFRLGGYAAQFDIFSFDFLSPENMTLVAGLSWAFGQLKVTETTKGGTTTCKNP
                     YFAPEFRTEFSMRLGDHFFFGLRGAYRADISKTGWKESGINNPALTGTKLSGWMTGAF
                     IGFGV"
     gene            complement(2007..2399)
                     /locus_tag="VFU15_05895"
     CDS             complement(2007..2399)
                     /locus_tag="VFU15_05895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717340.1"
                     /translation="MKKYGTYLLLFTVFTAAVFVALYFWNANGPARWHVDNFAWIPGI
                     IAAVSISIHFYLISSKKKRPAVFVRRFMAISTLRMIVYLAIIIGYIFRGGPSVAAFII
                     QFGFAYFCFMIFEVATLYQDMRKPVENE"
     gene            complement(2582..2815)
                     /locus_tag="VFU15_05900"
     CDS             complement(2582..2815)
                     /locus_tag="VFU15_05900"
                     /inference="COORDINATES: protein motif:HMM:NF021073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HEU4717341.1"
                     /translation="MTPQKPKPSSPKKRLNGYAKYLSMATYMAVIITLGTLGGRWLDG
                     RFHFVKFPVFTLFLSLLSVFAAMWYFIRDFIRK"
     gene            complement(2769..>2996)
                     /locus_tag="VFU15_05905"
     CDS             complement(2769..>2996)
                     /locus_tag="VFU15_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014082781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEU4717342.1"
                     /translation="TCQNADVSGNVRGKIHVAELLALKATSKLNGEINTGKLSIEPGA
                     DFSGSCSMGGVVKEIQHDSAKAKAELAEKTA"
BASE COUNT          784 a          740 c          826 g          646 t
ORIGIN      
        1 tcgcggaaag catggtgaac acgtacgcct ggaggaacgc aacaagaatg tcgagcaccg
       61 tcatgaatac ggtgaacgcc accgagccga tggaaacgcc gagtcctgcg cctaacgctt
      121 cgccgccttt gccgaagatg aagatgaggc tgaagaaaga aagcgcgatg atgtggccgg
      181 caagaatgtt cgcgaatagt cggatcatca ggaccagcgg cttcaggaac atgcccatga
      241 tctcgatcgg tgtgaggatg aaaagcacgc cgaacggaac gcctggcatc gcgaagatgt
      301 ggcgccagta atttttattc gcgctgaacg tggtgatgac aaacgtcatc agcgcaagcg
      361 tcatcgtgat ggcgatgttg ccggtgacat tcgcgccgcc ggggaaaacc gggatcatgc
      421 cgagcaggtt cgagatccag atgaagaaaa atacagtgag caggaacggc atgtaacgtt
      481 cgtatttcgg cccgatgctc ggacgcgcaa tgtcgtcgcg cacgaaaaga atcagcggct
      541 cgaaaagcga ctgcatgcct ttcggcgcct gtcccggccg gcggcgatac gctttcgcta
      601 cattgaggaa cacgaggaac atgatgccca cgatcacgaa aaggctcacc acgttcttcg
      661 tgatggaaat atcgaaaatt gattttgagg cgacctggtt ttgcgccatg gcgacagaat
      721 cccacacgat gatcttcccg tccttgttgt atgcataagt gtaatatttt ccctggtacg
      781 gctgaagctc gtgcgtttga ggattacgga aatttccgga agaaaaacat tcgaaccctt
      841 tatcggtttt cagaatgacg ggaagcggga tgcggatatt tccgaaaatg tgccattcgt
      901 gagaatcgga cacgtgctcc atgatcattt cgcccgggtt gaacccttct tttttcggcg
      961 cgccggcatc cgccacctta acattgcttg ccgaagaatc gctggcctgg tcttccattt
     1021 catagcagaa accgaaggcc gggatgagga gggcggcgag gaaaagagag aaccggttga
     1081 taagggcgtt tgaacgcatt gatagaaagc tgtttgacaa tgaaaatcga ggattttcgc
     1141 gcaattcggc tgcaaaaata ggtatttctt ttcctcgtgt taaaactttt cacaaaggtt
     1201 ttttggacaa aagatcaact ttagaaagaa tccaaaacaa aagcgggaac agccccgaaa
     1261 gccgttcccg cagaaagaag tttttgcccg atgaactaca cgccgaagcc gatgaatgcc
     1321 cctgtcatcc acccgctcag ttttgtgccc gtaagagcgg gattatttat tcccgactct
     1381 ttccagcctg tttttgaaat atccgcccgg taagccccgc gcagaccgaa gaaaaaatga
     1441 tcgcccaggc gcatgctgaa ttccgtccgg aactcgggtg cgaagtacgg gtttttgcag
     1501 gtcgtcgttc cgcctttcgt ggtttccgtc actttcagct ggccgaacgc ccaggaaagt
     1561 cccgctacga gcgtcatgtt ctccgggctg aggaaatcaa agctgaatat gtcgaactgc
     1621 gcggcatatc cgcccagcct gaacgtcagc gaatcaccgg ctttcacggt ttgcggaaga
     1681 aggtaatgga atgaatacag cgaagatccg ccgcggggaa gacggtcgtt gctgacgtgt
     1741 gcggaataat cgagacccac gcccattgca ttaccgaaac ccgttttcac gccgtacccg
     1801 cccagctgcg cgttcacgct ggccatggga aggaaataat acccgaagcc gggaccgagc
     1861 cagtacgatt tgacgtcgtc gctttttccc tgcggtgtca gcgccgaaag cgtatttacc
     1921 tgaaatgtga taagggcgag cgagaagatc agcagtgttt ttttcatatc gtttttttgg
     1981 agttgaatgc caaagtaagc aaagcttcac tcgttttcaa ccggcttacg catgtcctga
     2041 taaagcgttg caacctcgaa gatcataaag cagaaatagg caaagccgaa ctggatgatg
     2101 aaggccgcaa cggaaggtcc gccgcgaaaa atataaccga tgatgatcgc caggtaaacg
     2161 atcatgcgca acgtgcttat cgccatgaac ctgcgtacga agaccgcggg ccttttcttt
     2221 ttcgaagaga tgagataaaa atggatggaa atggaaacgg ctgcgatgat cccgggtatc
     2281 cacgcgaaat tatccacgtg ccatctggca ggaccattcg cattccagaa atacagcgca
     2341 acgaaaaccg ccgccgtaaa aacggtgaaa agcaaaaggt acgttccgta ctttttcatg
     2401 ctgtaaaagt agaaacgaat cggacatgaa aattgttctt tagggagcgg tattatccgc
     2461 cgaatacgcc ggcctcattg taaaaggctt caggcggggt aaaaaaaatc gcgggcgccc
     2521 gttcccgcag aacaggtggc ggctatgaag aggagcgcaa gcaggaattt ttcaccgtga
     2581 tttatttccg gataaaatcg cggatgaaat accacatggc cgcgaacacg gaaagcaggg
     2641 aaagaaacag cgtaaaaacc gggaatttca caaaatgaaa acgcccgtcg agccagcggc
     2701 ccccgagcgt tccgagcgtg atgatgacgg ccatgtaggt ggccatggaa agatatttgg
     2761 cgtagccgtt aagccgtttt ttcggcgagc tcggctttgg cttttgcgga gtcatgctga
     2821 atctctttca ccacgccgcc catgctgcag gagccggaga aatcggcgcc gggttcgatg
     2881 gacagcttgc cggtgttgat ctcgccgttg agcttggaag tggccttgag cgcgagaagc
     2941 tccgccacgt gaattttgcc gcggacgttc ccggagacat cggcattctg gcaggt
//
LOCUS       DASTSF010000201         6172 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_543119,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000201 DASTSF010000000
VERSION     DASTSF010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 6172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6172
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_543119"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            87..1013
                     /locus_tag="VFU15_05910"
     CDS             87..1013
                     /locus_tag="VFU15_05910"
                     /inference="COORDINATES: protein motif:HMM:NF040196.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5777 family beta-barrel protein"
                     /protein_id="HEU4717343.1"
                     /translation="MKKILFFLFLFTAPAAFAQIDLDSMANSMANSAPPAREFVRATF
                     KTTRIINFPNTENVGKHILDFRISHRFGDLNSGAYNAFGLDGPACIRIAFDYGITNYL
                     QVGIARTSIDKLIEGSAKWRILRQTTDNKMPVSLTWYATMNYTAMKDPNAAITGYDKY
                     NHLVDRFSYAHCLMLGRKFSDKFSIELNGFFIHYNIVDNILDKNDIFAVGFSGRYKLT
                     SRTAITWEYAYRINKYNSNPAISYYDPIGVGFDIETGGHVFQVHITNAFGMNEVQYIP
                     FSTSNPFKGGLRVGFNISRVFTTGKKDPNNTW"
     gene            complement(1046..1498)
                     /locus_tag="VFU15_05915"
     CDS             complement(1046..1498)
                     /locus_tag="VFU15_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017843417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEU4717344.1"
                     /translation="MTHRYNVRAYALVMHGGKLLVADEFFRGIRMTKFPGGGNEWGEG
                     LAETVKRELLEETGQEPVSIAHFYTTDFFVESAFHENVQLLSVYYTAALPHPENVPVK
                     TKPFDFDEEKDGAMVFRWINLDEISPDTFTFPIDKKVAELLKKKNPGR"
     gene            complement(1501..3231)
                     /locus_tag="VFU15_05920"
     CDS             complement(1501..3231)
                     /locus_tag="VFU15_05920"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HEU4717345.1"
                     /translation="MTFTRLFLVASFFIPACGFAQKRKVFTTTPVYDSVIAAYTSLAQ
                     KYPAMTQLKTYGTTDVGRPLHLFVISKDGKFTPADHATKTVILINNGIHPGEPDGIDA
                     CIQLSQDLLSQPALLPDNVEICIIPVYNIGGCLNRGACSRVNQNGPAEYGFRGNAKNL
                     DLNRDFIKCDAQNTWAFETIFQEWQPDIFVDTHVSDGADYQYVMTLIPTQRNKLHPVM
                     ADYMENMILPDLYKRMKEKKYEMCPYVETHGETPDSGIDAFLETPRYSTGYTALFNCI
                     GFVTETHMLKPYNDRVWATYEFLRAIIDIGSRNCETMGNARRTADRMVANEKIFALNW
                     KLDTASYDWFEFKGYEAKHKPSEFSGTRLYYDEKSPYTKKIKYYNTYTAIDCVAVPRY
                     YFVPQAYTAVIDRMKLNRVAMKKLAKDTAIWCGMYYVTDYKSPKGPYESHFLHSAVSV
                     SRDSQLVMFYKGDYIIETNQPCNRYIVETLEPKAADSFFAWNFFDGILQQKEWFSDYV
                     FEDKADSILKHDATVKKQFDDKMNTDTAFAHDHWGQLNFIYHHSAYMERTYDRYPVGR
                     LNGEMKLPED"
     gene            complement(3283..3897)
                     /locus_tag="VFU15_05925"
     CDS             complement(3283..3897)
                     /locus_tag="VFU15_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019598958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU4717346.1"
                     /translation="MRQIAGIGACLLFMLPLRTEQTGTPLQDAFSKSYTAEASGKYDD
                     AIKALSSVCDDGSYECSLRLGWLSYEAKDYVKSIAYYQKAIDAKPYAIEARFGIVKPE
                     SALLKWDDVLGQYMEILKIDPQNTKANYWTGCIYYNRKDYKKAISYFEKVVNLYPFDY
                     DSSIMLGWSYLNDGRSSDAKVMFGKVLLISPGDTSATGGLKKLN"
     gene            complement(3903..5120)
                     /locus_tag="VFU15_05930"
     CDS             complement(3903..5120)
                     /locus_tag="VFU15_05930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU4717347.1"
                     /translation="MHGRKITYLLLLVFHCAAAQRMDYRTADSLTWKYYLAGEWAKLD
                     SAGDLALGDHVDYPYLRMRLGYSAFVNGNYSRSLLHYEKALKEDKGNSFAAYYLAQNN
                     RNLGRDDEAAFYASRTDDSLARKTGLRKYDLSRSADGEMSVGFPSVATRSDAVYARFG
                     FGTQIGWRLSMYNSVDVFRQDFTSTLVDTAFRPPPGFPPPPHPPVKQVVEAVRQYRYY
                     TKWNYQLNGHFSVWASGNFSKQSDAGTISHFYIGMGGVNYAFAYGSISGESAYGKLEG
                     NTFTQYGGSFVVYPSGNLGWYVFMHGALTNDGGRTGYDVTPGFGFSPGKKIWMEARVN
                     FGNSQSYLSGEGYYFYRLIDQPVWSGTLSAGFPLGNNVQLMVHYNYSQSAQKDNNSNY
                     YQNTITGGLSWKR"
     gene            complement(5102..>6172)
                     /locus_tag="VFU15_05935"
     CDS             complement(5102..>6172)
                     /locus_tag="VFU15_05935"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEU4717348.1"
                     /translation="SKALDFMILDDEMKTYSGNGTQCRDYYCYAKPVLAPGDGIVEEA
                     VDGVEENAIGNVNMVQNWGNTIVIRHLPGLYTKLSHLKNGSLKVKKGDFVRKGDIIAQ
                     CGNSGRSPEPHIHFQVQAFPDVAARTLSYPLAYYFLHEKGKTSLESFSVPAEGKIISA
                     AETSTLLGAAFAFQPGFRQQFVFSENKKQKKESWETVTDAYNRTYIYVVGGNAYAYFI
                     NNGTVFYFTAFYGDKNSLLYRFYLSCYHIPLVQYGNMNATDHFPLHLFAGGPAKWLQD
                     VVAPFHVFVRKEFRMECAKSDDEYHPREITLSSSIRNFFPGRSVQSSGSEITVADGKI
                     RRFRFSDMENGNIIDAECTEEK"
BASE COUNT         1430 a         1714 c         1506 g         1522 t
ORIGIN      
        1 tgccgcccaa tttcaaaatc gacacgtgtc gcatcacaca atttgcaaaa tggatagcgg
       61 cgggagcgcc acaaaattaa actgccatga aaaaaatcct cttcttcctt ttccttttta
      121 ccgcccccgc cgcatttgcc cagatcgatc tcgacagcat ggcgaacagc atggcgaatt
      181 ccgcaccacc tgcgagagaa ttcgtacgcg caacgttcaa aacaacgcgg atcatcaatt
      241 ttccgaatac ggaaaatgtc gggaaacaca ttctcgattt ccgcatctcg caccgtttcg
      301 gcgatctcaa ttccggcgcg tacaatgcgt tcggtctcga cggcccggca tgcatacgca
      361 ttgcgttcga ttacggcatc acgaattacc tgcaggtcgg catcgcgcgc acgtcgatcg
      421 acaagctgat cgaaggttct gcgaaatgga gaatcctgcg gcaaaccacc gacaataaaa
      481 tgccggtttc gctcacgtgg tatgcgacga tgaattacac ggcgatgaaa gacccgaacg
      541 cggcgatcac cgggtacgac aagtacaatc acctggtgga ccgtttttcg tacgcgcact
      601 gcctcatgct cgggagaaaa ttctcggata aattctccat cgagctgaac ggttttttta
      661 tccattacaa catcgtcgac aacatcctcg acaaaaacga tattttcgcg gtcgggtttt
      721 ccggacgcta caagctcact tcgcgcactg cgatcacctg ggaatatgcg taccgcatca
      781 acaagtacaa cagcaatccc gccatcagct attatgatcc catcggcgta ggtttcgaca
      841 ttgaaacggg cggccacgtt ttccaggtgc acatcacgaa tgcattcggc atgaacgagg
      901 tgcagtacat cccgttcagc accagcaacc ctttcaaggg cggactgcgc gtaggcttca
      961 acatttcacg cgtgttcacc acgggcaaga aagacccaaa caacacgtgg taagaaaacg
     1021 tacggaagta aaacaaaacc ctggtctacc ggccggggtt tttctttttc agcagctctg
     1081 cgactttttt gtcaatggga aaagtgaaag tgtccggaga aatttcatcg aggtttatcc
     1141 agcgaaaaac catcgccccg tctttttcct catcgaagtc gaacggtttc gttttcacag
     1201 gaacgttctc cgggtgcggc aacgccgctg tgtaataaac actgagcagc tgtacatttt
     1261 catggaacgc cgattccacg aaaaaatccg tggtgtaaaa atgcgcaatg gaaacgggct
     1321 cctgccctgt ttcttcgagc agctcgcgct tcacggtttc cgcaagacct tcgccccatt
     1381 cgttgccgcc cccgggaaat ttcgtcatgc gtatccccct gaaaaattca tcggcgacga
     1441 gcagtttccc cccgtgcatc accagcgcgt acgcgcgtac gttgtacctg tgcgtcatgt
     1501 tcaatcctcc ggcagtttca tttccccgtt cagtcttccc acgggatagc ggtcgtacgt
     1561 tcgttccatg tacgcggaat gatggtaaat gaaattcaat tgtccccagt gatcatgggc
     1621 gaaagcagta tccgtgttca tcttatcatc gaactgcttt ttgacggtcg cgtcgtgctt
     1681 taaaatgctg tcggccttgt cttcaaaaac gtaatcgctg aaccattctt tttgctgcag
     1741 tatcccgtcg aaaaaattcc atgcgaaaaa actgtccgcc gctttcggtt ccagcgtctc
     1801 gacaatgtac cggttgcacg gctggttcgt ttcgatgatg taatcgcctt tgtaaaacat
     1861 caccagctgc gaatcgcggg aaacactcac cgccgaatga agaaaatgcg actcgtacgg
     1921 gcctttcgga cttttgtaat ccgtcacgta atacatcccg caccagatcg cggtatcctt
     1981 cgccagtttt ttcatcgcca cgcggttgag cttcatgcgg tcgatcaccg cggtgtaagc
     2041 ctgcggcaca aaataataac gcggcaccgc cacgcagtcg atcgccgtat acgtgttgta
     2101 gtattttatt tttttcgtgt acggggattt ctcgtcgtag tacaaacgcg ttccggaaaa
     2161 ttccgaaggc ttgtgcttcg cttcgtaacc tttgaattcg aaccagtcgt agctcgccgt
     2221 gtccagcttc cagttcagcg caaatatttt ttcattcgcc accatgcggt cggctgtccg
     2281 ccgcgcattt cccatcgtct cgcaattcct gctgccgata tcgatgatgg cccggaggaa
     2341 ttcatacgtc gcccacacgc gatcgttata cggcttcagc atgtgcgttt ccgtcacgaa
     2401 gccgatgcag ttgaacagcg cggtatagcc cgtggaataa cgcggcgttt cgaggaacgc
     2461 gtcgatgccc gaatccggcg tctcgccatg cgtttccacg tacgggcaca tttcgtattt
     2521 cttttccttc atgcgtttgt agagatcggg caggatcatg ttctccatgt aatccgccat
     2581 gacaggatgc agcttgttac gctgcgtggg aatgagcgtc atcacatact ggtaatccgc
     2641 gccgtcggaa acgtgcgtat cgacgaaaat atccggctgc cattcctgga agatcgtttc
     2701 gaacgcccac gtattctgcg cgtcgcattt gatgaagtcg cggttgagat cgaggttttt
     2761 cgcattgccg cggaagccat attctgcggg tccgttctgg ttgacgcgcg aacaggcgcc
     2821 ccggttgaga cagccgccga tgttgtacac cgggatgatg cagatctcta cattatccgg
     2881 aagcagcgcc ggctgcgaaa gaagatcctg tgaaagctgt atgcacgcgt cgatgccgtc
     2941 cggctccccg ggatgaattc cgttgttgat gagaatgacg gttttcgtgg catgatcggc
     3001 aggtgtgaac ttcccgtctt tcgagatcac gaaaagatgg agcggcctgc ccacgtccgt
     3061 cgtgccgtac gttttcagct gcgtcatcgc cgggtatttc tgcgccagcg aagtgtacgc
     3121 ggcaatgacg gaatcataga ccggcgtggt cgtgaaaact tttcgcttct gcgcgaaacc
     3181 gcatgcaggg atgaaaaacg aagcgacgag gaaaaggcgg gtgaaagtca tgccctaaat
     3241 tacttttccc atcgcaaacc accatcaaaa aatcacaggc ggtcagttca gctttttaag
     3301 cccgccggtc gcgctcgtgt cgccgggact gatgagcagc actttaccga acatcacttt
     3361 cgcgtcggag ctgcggccgt cattgaggta gctccagccg agcatgatgg acgaatcgta
     3421 atcgaacggg tacaggttga ccactttttc gaaatacgag atcgcctttt tgtaatcctt
     3481 gcggttgtaa taaatgcagc cggtccagta attcgctttc gtgttctgcg gatcgatctt
     3541 gagtatctcc atgtactggc cgagcacatc gtcccatttc agcaatgccg attccggttt
     3601 cacgatgccg aaacgcgcct cgatcgcata cggtttcgca tcgatggctt tctggtaata
     3661 cgcaatggat ttcacataat cctttgcttc gtaactcagc cacccgagtc gcagcgagca
     3721 ttcgtagctg ccatcgtcgc aaaccgacga cagcgctttt atcgcatcgt cgtatttgcc
     3781 ggaagcttcg gccgtatagc ttttcgagaa tgcgtcctgc agcggcgttc ccgtttgttc
     3841 cgtgcgcaac ggaagcatga agagcaggca ggcgccgatg cccgcgatct gtctcatatt
     3901 tttcatcttt tccatgatag acctcctgtt attgtgtttt gataataatt gctgttgttg
     3961 tctttctgcg ctgactgcga ataattgtaa tgcaccatca gctgcacgtt atttcccaat
     4021 ggaaaaccgg cggaaagcgt tccgctccac acgggctggt cgatcagcct gtagaaataa
     4081 tatccttcgc cgctgaggta cgattgtgaa ttcccgaagt tcacacgcgc ttccatccaa
     4141 atttttttcc ccggactgaa cccgaagccg ggcgtgacat cgtacccggt cctgccgccg
     4201 tcatttgtca gtgcgccgtg catgaaaacg taccagccga gatttcccga aggataaaca
     4261 acgaacgaac cgccgtactg cgtaaacgta tttccctcca gcttcccgta agcactttcc
     4321 ccggaaatgc tcccgtatgc gaacgcatag ttcacgccac ccatcccgat gtaaaaatga
     4381 ctgatcgttc ccgcatccga ctgcttcgag aaatttcccg aagcccacac tgaaaaatgc
     4441 ccgttcagct ggtaattcca tttggtgtaa taacggtatt gccgcacagc ttccaccacc
     4501 tgcttcaccg gcggatgcgg aggcggagga aatcccgggg gcggacggaa cgccgtatcc
     4561 accagcgtcg aagtaaaatc ctgccggaaa acatccacag aattgtacat gctgagccgc
     4621 cacccgatct gcgtaccgaa accgaaacgt gcatacacgg catcggaacg cgtggccacc
     4681 gaaggaaaac ccacgctcat ttctccgtct gcgctgcggg aaagatcgta tttcctcagg
     4741 ccggttttcc gcgccagtga atcatccgtg cgtgatgcgt aaaacgcggc ttcgtcatca
     4801 cgcccgagat tacggttgtt ctgcgcgagg taataggctg cgaatgaatt ccccttgtct
     4861 tctttcaatg ccttttcata atgaagaagg ctgcggctgt aatttccgtt cacaaaagcg
     4921 gaataaccaa gacgcatgcg caggtacgga taatcgacat gatcgcccag cgcaagatct
     4981 cccgcgctgt cgagcttcgc ccattcaccc gcgaggtaat acttccacgt caggctgtcc
     5041 gcggtacggt aatccatccg ctgtgcggca gcacaatgaa aaaccaggag aagcaggtat
     5101 gttatttttc ttccgtgcat tccgcgtcga ttatatttcc attttccatg tcgctgaacc
     5161 ggaatctccg gatctttcca tccgcaacgg tgatttccga cccggagctt tgcacgcttc
     5221 ttcccgggaa aaaattccgg atcgaggagc tcagcgtgat ctcgcgggga tggtattcat
     5281 cgtcgctctt tgcgcattcc atcctgaatt ccttccggac gaaaacatgg aacggcgcga
     5341 caacatcctg cagccatttc gccggaccac ctgcaaaaag atgaagcgga aaatgatccg
     5401 ttgcgttcat attcccatac tggacaagcg ggatatggta gcaggaaaga taaaagcggt
     5461 acagcaacga atttttatcg ccgtaaaatg cggtgaaata aaaaaccgtc ccgttattga
     5521 tgaaatacgc gtatgcattc ccgcccacaa catagatgta tgtccggttg tacgcgtccg
     5581 tcactgtttc ccagctttcc tttttttgct ttttgttttc cgaaaaaaca aattgctgcc
     5641 ggaagccggg ctgaaatgca aatgcagcgc caagcagcgt gctcgtctcc gctgcagaaa
     5701 taatttttcc ttcggcaggc acggaaaacg attcgagcga cgtcttccct ttttcatgca
     5761 gaaaataata agccagcgga tagctgagcg tgcgcgccgc cacatcggga aaagcctgca
     5821 cctggaaatg aatgtgcggt tcaggcgagc gtcccgaatt tccgcattgc gcgatgatgt
     5881 cgcctttgcg cacgaaatcc cctttcttca ctttgagcga accgttcttc aggtgggaaa
     5941 gtttcgtgta gagtccgggg agatggcgga tcacgatcgt gttgccccag ttctgcacca
     6001 tgttcacgtt accgatcgca ttttcttcca cgccatcgac ggcttcttcc acgataccgt
     6061 ctcccggtgc cagaacaggt tttgcgtagc agtaataatc cctgcattgt gttccgttgc
     6121 ccgaatatgt tttcatttca tcatcgagga tcataaagtc gagcgccttc ga
//
LOCUS       DASTSF010000202         3590 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1770673,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000202 DASTSF010000000
VERSION     DASTSF010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3590
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1770673"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1062
                     /locus_tag="VFU15_05940"
     CDS             <1..1062
                     /locus_tag="VFU15_05940"
                     /inference="COORDINATES: protein motif:HMM:NF024490.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HEU4717349.1"
                     /translation="KKIQITVYRVAKNSNIMNALINAVKNGKEVVVIMELQARFDEEN
                     NIYWANRLQEEGARVIFGVPGIKVHSKLFLISRREEGKMISYVHIGSGNLNEWTARIY
                     TDHTLLTSDKRITEEVQNVFDFYNDNLKPGSYKHLYVSPFTMRKRFLNLIQKEIDNAR
                     EGNAAWIILKLNNLTDREMILKLYEASQSGVRIRLVVRGACAVVPGVKGFSENIEAIS
                     IVGRFLEHSRVLIFCNGGEEKYFISSADWMTRNLDHRSEVAVPIYDKESQQELKHLIN
                     LQLRDNRKSRTLGGKSENEYRKSAAQKPLIAQEEIKSWLEKKAGSSGRTVRKPKVEVR
                     SDRHRVKRRKAAVRKRIRK"
     gene            992..1900
                     /locus_tag="VFU15_05945"
     CDS             992..1900
                     /locus_tag="VFU15_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014680051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HEU4717350.1"
                     /translation="MKFAAIDIGSNAVRLLFGNVYESKSGPVFKKADLVRIPLRLGDD
                     AFLHKKLSEEKIRKLVLAMRAFRQLLDFYDVVDYKACATSAMREATNSIDVIKRVAVE
                     AGIAIEIIDGKTEAQIIYSNHVAENLDPSVNYLYIDVGGGSTELTLFSRNHVIASKSF
                     NIGTIRLLNDLVSKELWNDFKDWIKTNTKDFLPLQGIGSGGNINKIFKMSRKKQNRPL
                     SYSKLKEMHEFVSSFSLQERMEVLGLNPDRADVIVPAGKVFLTVMKAANMDKIYVPQI
                     GMADGLIHLLYENHLARSRKGLVFSE"
     gene            1968..2744
                     /locus_tag="VFU15_05950"
     CDS             1968..2744
                     /locus_tag="VFU15_05950"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEU4717351.1"
                     /translation="MQNPAMIILRFLRAIFGFYAVIVFAALLIVFSILYAVVFLVLPE
                     KSAPHFAHRVLSRTWAKMLQVFFFLPVRIENKNLIDPGKTYVFVANHRSQLDIPVYAI
                     ACRNTFRFLAKAELAKIPLMGFIIRKLYLTVNRADRADRNRSIEVMKRSIDDGISVFL
                     CPEGTRNRHDEPPVMEFRDGAFRLAVLTQTPLAVLTLFNTGEKLSPLRPVELAPGTIY
                     GTWDEPIETKGMTLDDVPALKEKVRSMMIAHLREYRGGKS"
     gene            3020..>3590
                     /locus_tag="VFU15_05955"
     CDS             3020..>3590
                     /locus_tag="VFU15_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014033798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HEU4717352.1"
                     /translation="MDFDALLKLLQDWTNRVPLIILGSGASVPFNLPSMWTLGAHLKK
                     SISFADKDDQKQFEEFKVYFDKVNDLEATLLQFQLRSNVLSQIIFETWKLVNKHDLEA
                     YEQILNTKIEFPLADLTNHLLSTAQKKLSIVTTNYDRLAEYAASLSHAFICTGYTQNY
                     FGHFSKLIHQQDLSKLNGFSGQVNIWKVHG"
BASE COUNT          964 a          925 c          882 g          819 t
ORIGIN      
        1 aaaaaaatcc agatcaccgt ttaccgcgtg gcgaagaatt cgaacatcat gaacgcgctg
       61 atcaatgccg tgaagaacgg caaggaagtg gtggtgatca tggagctgca ggcgcggttc
      121 gacgaggaga acaacatcta ctgggcgaac cgcctgcagg aggaaggcgc acgggtgatc
      181 ttcggcgtgc cgggaatcaa agtgcattcc aagctgttcc tcatttcacg aagagaagaa
      241 ggaaagatga tctcctacgt gcacatcggt tcgggcaacc tgaacgaatg gaccgcgcgc
      301 atttacaccg atcacacgct gcttacgtcc gacaagcgca tcacggagga agtgcagaac
      361 gtttttgatt tttacaacga caacctgaaa cccggaagtt ataaacacct ttacgtgtcg
      421 ccgttcacca tgcgcaagcg ctttctcaac ctcatccaga aggagatcga caatgcgcgg
      481 gaaggaaatg cggcctggat cattctcaag ctcaataatc ttaccgaccg agaaatgatc
      541 ctcaagctgt acgaagccag ccagtcgggt gtgcgcatcc gcctggtcgt gcgcggtgcc
      601 tgcgccgtgg tgccgggtgt gaaagggttc agcgagaaca tcgaagcgat cagcattgtc
      661 gggcgtttcc tcgagcacag ccgggtgttg atattctgta atggcggaga ggaaaagtat
      721 tttatatctt cggccgactg gatgacgagg aacctcgatc accgctcgga agtggcggtt
      781 ccgatttacg acaaggaaag ccagcaggaa ttgaaacacc tgatcaacct ccagctccgc
      841 gacaacagga agtcgcgcac actcggggga aaatcggaaa atgaatacag gaaatccgct
      901 gcgcagaaac cgctcattgc gcaggaagaa ataaaatcat ggctggagaa aaaagcggga
      961 tcttccggcc ggacagtaag aaaaccaaag gttgaagttc gcagcgatcg acatagggtc
     1021 aaacgccgta aggctgctgt tcggaaacgt atacgaaagt aaaagcggtc ccgttttcaa
     1081 aaaagccgac ctcgtccgca tccctctccg tctcggcgac gacgcattcc tccacaaaaa
     1141 actttcagaa gaaaagatcc ggaagctggt cctcgccatg cgcgcgttcc gccagctgct
     1201 cgatttctac gacgtggtgg attacaaagc ctgcgctaca tcggccatgc gcgaagcgac
     1261 gaacagcatc gacgtgatca agcgcgtcgc cgttgaagcg ggcatcgcca tcgagatcat
     1321 cgacgggaaa acggaagcgc agatcattta cagcaaccac gtggcggaaa acctcgatcc
     1381 ttccgtcaac tatctctaca tcgatgtcgg cggcggaagc acggagctca cgcttttttc
     1441 ccgcaaccat gtcatcgcct ccaaatcgtt caacatcggc accatccgcc tgctgaacga
     1501 tctcgtgagc aaagagctgt ggaacgattt caaggactgg atcaaaacga acacgaagga
     1561 ttttctcccg ctgcagggga tcggttcggg cggcaacatc aacaagatct tcaagatgtc
     1621 gcgcaagaag cagaaccgcc cgctttccta cagcaagctg aaggagatgc acgagttcgt
     1681 ctcttctttt tcattgcagg aacgcatgga agtgctcggc ctcaatcccg accgcgcgga
     1741 cgtgatcgtt ccggcaggga aagtttttct caccgtcatg aaagccgcga acatggacaa
     1801 gatctatgtc ccgcagattg gcatggccga cgggctcatt cacctgctgt acgaaaatca
     1861 tctcgcgaga agccgcaagg gtttggtgtt cagcgagtga atgtgctaca ttcgcgtttc
     1921 ccgtttttct tcactcgttt tttgatacga attcgctgaa aaacagcatg caaaatccgg
     1981 cgatgatcat tctccgtttc cttcgcgcaa ttttcgggtt ctacgctgtg atcgttttcg
     2041 cagcgctgct gatcgtgttc agcattctct acgcggtcgt gttcctcgtt ctgccggaaa
     2101 agtccgcgcc gcatttcgcg caccgcgtgc tctcgcgcac ctgggcgaag atgctgcagg
     2161 tttttttctt cctgcccgtg cggatcgaaa acaaaaacct tatcgatccc gggaaaacgt
     2221 acgtcttcgt tgccaaccac cggtcgcagc tcgacattcc cgtgtacgcg atcgcgtgca
     2281 ggaacacgtt ccgctttctc gccaaggccg agctggcgaa gatcccgctc atgggcttca
     2341 tcatccggaa gctctacctc accgtgaacc gcgccgaccg cgccgaccgc aaccgcagca
     2401 tcgaggtgat gaaacgctcg atcgacgacg gcatttctgt tttcctctgc ccggaaggca
     2461 cgcgcaaccg ccacgacgaa ccgccggtga tggaattccg cgacggcgca ttccggcttg
     2521 ccgtgctcac gcaaactccg cttgccgtgc tcacgctttt caataccggt gaaaaattat
     2581 cgccgctgcg ccccgtagag cttgcacccg gaaccattta cggaacgtgg gacgagccca
     2641 tcgaaacaaa aggcatgacg ctggacgacg ttcccgcgct gaaagaaaaa gtgcgcagca
     2701 tgatgatcgc acatttgcgg gaatacaggg ggggaaagtc ttaacgttca gctatgaaaa
     2761 tgaacggact gcagattcgt ttgctgccga gctcttaagt tatcgctacc agcagttgtt
     2821 gggatattcc agttgtaagg ccattgaaat ttacgagaat atgagtgtac actgtttact
     2881 gacgattatt tcgccatttg aagcattgta aaaatgacta aatttctaaa agaaataatg
     2941 cgctataaag tagcgcccat aaacgagctg gctgtaatta tttgtgacat tgcaaatatc
     3001 aaactgatag acattacata tggattttga cgcattacta aaactacttc aagactggac
     3061 aaatagagtt cctctaatca ttcttggcag tggtgcatct gttccgttta accttccttc
     3121 tatgtggacg cttggcgccc atttgaaaaa gtcaatttcg tttgccgaca aagacgacca
     3181 gaaacaattt gaagaattta aagtttactt tgataaggtt aatgatttgg aagccacatt
     3241 gcttcaattt caactccgat caaatgtttt atcgcagata atttttgaaa cttggaagtt
     3301 agttaacaaa catgatcttg aagcatacga acaaatttta aatacgaaga ttgagtttcc
     3361 actcgcagat ttgacaaatc acctactctc aactgcacag aaaaaacttt ccatcgtaac
     3421 tacaaactat gaccgacttg ctgaatatgc agcaagctta tctcatgcgt tcatctgcac
     3481 aggttatacg caaaattatt tcggacactt ttctaaactg atacatcaac aagacttaag
     3541 caaactaaac ggctttagtg gacaagtaaa catttggaaa gttcatggtt
//
LOCUS       DASTSF010000203         2358 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_487058,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000203 DASTSF010000000
VERSION     DASTSF010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2358
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_487058"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..769)
                     /locus_tag="VFU15_05960"
     CDS             complement(<1..769)
                     /locus_tag="VFU15_05960"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEU4717353.1"
                     /translation="MNKPAAELPGSGKDFLPQINLLRGIAAVMVAFYHFATFSGEKGD
                     LLDSHNLVKVSGHFGCQGVYIFFVISGFVIPWSMYNGKYTLRKAGRFLAKRSLRIEPP
                     YIVSFVLVILINVFLSKMWGGEYPVDPKRVAFHIGYLIPFVHSKYEWFNIVYWTLGIE
                     FQYYLTMTLLFPLLNHSNRYVRYSVMLFFLFMPFLYGDAGFLPLFGPCFLVGFVIFLM
                     KTDRMKWPEAIVFLAASLVMNYFFRDWEVMASTMFAFL"
     gene            complement(766..2220)
                     /locus_tag="VFU15_05965"
     CDS             complement(766..2220)
                     /locus_tag="VFU15_05965"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEU4717354.1"
                     /translation="MKKLLPLFLLLATVSVRSQTYITDTLMTGLYAPVAFDFTPDGRI
                     FLTQKGGSGSPAYQAQVKVYTLAGNYLGIFYDLTDSTDADFERGLIGIAVDPDYTVNH
                     YVYVYYVHNYTGDEHIRIARFTDSLNTGTHPTIIFDIDVADNLPGNHFGGNLHFRPTE
                     PDKIYFSIGDLGLNQTDTALNYAHMLTNPFGKTLRINKDGTIPADNPYYDDGNIYTGN
                     CDIIWSYGHRNAFDFCFSPVSDTFYCSENGLITWDEVNVIHRGRNYGWNECEGDYVNS
                     STTQLCDDTTSVLPIETWGAPLGGLTGILYYTGPVMPEFDDHLLVADNDYGNIFDLAL
                     GNAPAYDSVTSRTLWMDLTSTGGLTTIKEGPEGCVYAMYGGYTANGAIYRVCPSWMSV
                     NDPSGGNMDARIYPNPSAGAATLSYRLAQPGDVKITLCDIFGKEIAVLADESENDGTH
                     SLTIDAQQLHLPAGTYIVRIDENGFRQTLKLVIL"
BASE COUNT          564 a          599 c          674 g          521 t
ORIGIN      
        1 acaggaacgc gaacatcgtc gaggccatca cttcccagtc gcggaagaaa taattcatga
       61 ccagcgaagc ggcaagaaaa acaatcgctt ccggccattt catgcgatcg gttttcatca
      121 ggaagatcac gaaacccacg agaaagcacg gaccgaacag gggaagaaag ccggcgtcac
      181 cgtaaaggaa aggcatgaac aggaagaaga gcatgacgga atagcgcacg tagcggttgc
      241 tgtggttgag cagcgggaaa agcagcgtca tcgtcaggta atactggaat tctatgccca
      301 gcgtccagta gacaatgttg aaccattcgt attttgaatg aacgaaagga atgagatagc
      361 cgatgtggaa ggccacgcgt ttcggatcga ccgggtattc cccgccccac attttcgaga
      421 gaaaaacatt gataagtatg accagcacga agctgacgat gtagggaggc tcgatgcgca
      481 ggcttcgctt ggcgagaaaa cgtcctgcct tgcgcagcgt gtatttgccg ttgtacatcg
      541 accagggaat gacaaagccg gaaatgacga agaaaatgta aacgccctgg cagccgaaat
      601 gcccgctcac tttcacgagg ttgtggctgt cgaggagatc gcctttttcc ccgctgaaag
      661 tcgcgaagtg gtagaacgcc accatcactg ccgcgatccc gcgcaggagg ttgatctggg
      721 gtagaaaatc tttcccgctc ccgggaagtt cggctgcagg tttattcaca aaatgacaag
      781 cttgagggtt tggcggaaac cgttttcatc gatacggacg atgtatgttc ccgcaggaag
      841 atgaagctgc tgcgcatcga tcgtaagtga atgtgttccg tcgttttcgg attcatccgc
      901 caaaactgcg atttcttttc cgaatatatc gcataaagtg atcttcacat cgccgggttg
      961 tgcaagccgg taggaaagcg ttgcagcgcc ggccgaagga ttcggataga tgcgcgcatc
     1021 catattcccg ccggaaggat cattcacgga catccacgaa ggacaaaccc tgtagatcgc
     1081 cccgttcgcc gtgtaaccgc cgtacatcgc gtacacgcag ccttccggtc cttcctttat
     1141 cgtggtgagt ccgccggtgg aagtaaggtc catccataac gtgcgcgacg tgacggagtc
     1201 ataagccggt gcattcccca gcgcgagatc gaaaatgttc ccgtaatcat tgtccgccac
     1261 cagcaggtga tcgtcgaact ccggcatcac cgggccggta taatataaaa tgccggtgag
     1321 cccgccgagc ggcgcgcccc atgtttcgat cgggagcacg gacgtagtgt catcgcacaa
     1381 ctgcgtcgtg gaactgttca cgtaatcgcc ttcgcattcg ttccagccgt aattccttcc
     1441 cctgtgaatg acgttcactt cgtcccacgt gatcaggccg ttctcggaac aataaaacgt
     1501 atcgctcacc gggctgaagc agaaatcgaa tgcattgcgg tggccgtagc tccagatgat
     1561 gtcgcagttg ccggtgtaaa tattcccgtc gtcgtagtac gggttgtccg ccgggatcgt
     1621 tccgtccttg ttgatgcgca gcgtttttcc gaatggattc gtcagcatgt gcgcgtaatt
     1681 cagcgctgtg tccgtctggt tgaggccgag gtcgccgatg gagaaataaa ttttatccgg
     1741 ttccgtggga cggaaatgca ggttcccgcc gaaatgattt ccgggcaggt tatctgcaac
     1801 gtcgatatcg aaaatgatcg tcgggtgcgt gccggtgttg aggctgtcgg tgaaccgtgc
     1861 gatgcggatg tgctcgtcgc ccgtgtagtt gtgcacgtaa tacacgtaaa cgtaatggtt
     1921 gacggtataa tcagggtcga ccgcgatgcc gatcaggccg cgttcaaagt ccgcatcggt
     1981 ggaatcggtg aggtcgtaga aaatgccgag gtaattgccc gcaagcgtat acacttttac
     2041 ctgcgcctgg tacgccggcg atccgctccc gcctttctgc gtcaggaata tcctgccgtc
     2101 gggtgtgaag tcgaaagcca cgggcgcata cagtccggtc atcagtgtgt cggtgatgta
     2161 cgtctgcgaa cgaacggaaa cggtggcgag gaggaggaaa agcggtagca gttttttcat
     2221 gacagaaaag gccggatcag ttacccggcc ttaacgaaaa attgcgccgg atagtattta
     2281 ttcggtgacc gatattttga tgttctgcga atacgtcagc gcctgcatcg agacgaaata
     2341 attccccggc gcgagtcc
//
LOCUS       DASTSF010000204         3969 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_54349,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000204 DASTSF010000000
VERSION     DASTSF010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3969
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54349"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1310
                     /locus_tag="VFU15_05970"
     CDS             <1..1310
                     /locus_tag="VFU15_05970"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HEU4717355.1"
                     /translation="HITIRGNSIHDISFSANPGDPVTANTKSHPVLVTGNDTAIAISN
                     VLITGNEIFNCRTGVSDAVYISGNVDGFSVNANRVHDVSNDGIVVAGHTGSCADAADD
                     CPRNGDLRWNNTWDCVAGSASVAGISIDGAKKITVENNLSFGNGFGIEVTCRKTGETS
                     SDIIVRNNIVYNNLEGGLIIGGYNYPAGSGMVVSSSFTNNTIVNNYLSGNPNGEIIIV
                     FCEYVSIRSNIVYTISSSDPLVSANSGLTGLVLDDNLYYSTSAPQFSSGAGTAASLPA
                     WATLVSGEAHSVYGDPQFLDGSAMNFHLLASSPAIDAGDSSYVVSITETDMDTMMRVQ
                     NDRIDIGADEYGTAVGFFQGRNPASPKVLYDRVSGSLQVIYPSPARKNATVNLTGVDG
                     KVISSTEIEKGKNSVSFDVSELPVGTYIIYSDDMKYSGVKFVR"
     gene            complement(1314..2024)
                     /locus_tag="VFU15_05975"
     CDS             complement(1314..2024)
                     /locus_tag="VFU15_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEU4717356.1"
                     /translation="MQIKLFPASGRGHADHGWLNAYHSFSFAGWYDPDLVHFGLLRVL
                     NDDTIAGGMGFGKHPHDNMEIVTIPLEGALQHSDSTGRTEVIRKGEVQIMSAGSGIFH
                     SEVNASKSDPVKLFQIWVFPKLRNIAPRYDQKVFDEASRENRLCTVVSPDGNEGSLQI
                     NQDAWFSLGRFSGAQKIEYTIHRKGNGVFLMIVDGNASVEGNGLAKRDAIGLWETEKF
                     TVDVKAGCEVLLIEIPMN"
     gene            complement(2037..2492)
                     /locus_tag="VFU15_05980"
     CDS             complement(2037..2492)
                     /locus_tag="VFU15_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015811891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEU4717357.1"
                     /translation="MSLEDEIKQKQFSSIYQKLGLNILFTSSWLQTIQQRLFKPYGIT
                     TQQYNVLRILRGQHPNPASVGLIQERMLDRSSNASRLVDKLVDKKLVERNTCPKDRRQ
                     VDILISPAGLKLLSELDGVIAQHEETFRVLTEEEARTVSDLLDKFRKQH"
     gene            complement(2571..>3969)
                     /locus_tag="VFU15_05985"
     CDS             complement(2571..>3969)
                     /locus_tag="VFU15_05985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717358.1"
                     /translation="FYFEDVFMSFRNGNNWGEPEPLDSTVNGNGHDACIGISNDGSTL
                     LIYKDEEGNGDIYTSQLNGFYWSAPVPLKGDVNTDSWEGSATFSTDMHTMYFSSDRPG
                     GYGGRDIYVATLQPDGTWGNVKNMGPKINTPYDEDAPFLHPNGTTLIFSSQGHNSMGG
                     YDIFRTDLTPVDSTFWEPSDPVNVGYPINTAGDDKYFVLATDGKHGYYSSGKPGGNGQ
                     QDIYQVDYDFHLDQSNVMLLSGVITLDDQPVHASVFIHDDAGKLSMFHVSSNSETGKY
                     LITLPLGRNYNVTYELDSLHQKRTLDAMQTGQMQRSVIDIHFYTEAYAEKKRQDSLKT
                     VDTSTVAVVKKLPPDSVVNEGQVNANDYTDILTRYGNARSPGLLFHVQVAAYNFPDNY
                     QYKRLLPLGSIDKRVLDDGITRFTMGTFSTLAEAEAYRQKIIAAGQTDAFVTAEKDGK
                     RYLLKDLAELHFFQQ"
BASE COUNT          875 a         1044 c         1067 g          983 t
ORIGIN      
        1 cgcacattac gatacgcgga aacagcattc acgacatcag cttttctgcg aatcccggcg
       61 accctgttac ggcgaataca aagtctcacc cggtgctcgt taccgggaat gatacggcca
      121 tagcgataag caatgtcctg atcacgggca acgaaatatt caattgccgc acgggtgtga
      181 gcgatgccgt ttatatttcc ggcaatgtcg atggattttc cgtgaatgca aatcgcgtgc
      241 acgatgtttc gaatgacgga attgtcgtcg caggtcacac cggctcatgc gccgatgcag
      301 cggacgattg cccgaggaac ggcgatctgc gctggaacaa tacgtgggat tgtgtcgcgg
      361 gaagcgcttc cgttgcaggc atcagcatcg acggggcgaa aaaaatcacg gtcgaaaaca
      421 atctttcatt cgggaacggc ttcggcatcg aggtgacgtg ccgtaaaacc ggggaaacaa
      481 gctcggatat tattgtccgg aacaacatcg tttacaacaa cctcgaaggc gggctcatca
      541 tcggtgggta taattacccg gcgggatcgg gaatggtggt cagctcttcc ttcacgaaca
      601 atacgatcgt caacaattat ctttcgggta acccgaacgg agagatcatc attgtgttct
      661 gcgagtacgt ttccatccgc agcaatatcg tttatacgat ttcgtcgtca gatccgcttg
      721 tctcggccaa ttcgggtctc acgggtcttg tactcgacga caacctgtat tattccacgt
      781 ccgcacccca gttcagctcg ggagccggaa ccgcggcatc tcttccggcg tgggcgacgc
      841 tggtttccgg tgaagcgcat tcggtatacg gagatccgca gttccttgac gggagcgcga
      901 tgaattttca cctgctggct tcttccccgg ccattgatgc cggcgacagc tcgtacgttg
      961 tttccattac ggaaacggat atggacacga tgatgcgcgt gcagaatgac aggatagata
     1021 tcggcgcgga cgaatacggc actgccgtag ggtttttcca gggaaggaat cccgcatcgc
     1081 cgaaagtgct ttacgaccgt gtttccgggt cgctccaggt gatctatcct tcgcctgcaa
     1141 ggaagaatgc aacggtaaat cttaccggcg tggatggcaa ggtgatttct tcaacggaaa
     1201 tagaaaaagg gaagaattcg gtttcgttcg acgtgagcga gctgcccgtg ggaacgtaca
     1261 tcatctactc cgacgacatg aaatacagcg gtgtgaagtt cgtgcgctga agatcagttc
     1321 atcggaattt cgatcagcag cacttcgcat cccgctttca catcgaccgt gaatttttcg
     1381 gtttcccaca aacctatcgc atctcttttc gcgagcccgt tcccttccac cgaagcattt
     1441 ccgtccacga tcatcaggaa tacgccattg cctttacggt gaatggtgta ttcgatcttt
     1501 tgcgccccgg aaaagcggcc gagcgaaaac caggcatcct ggttgatctg cagggaaccc
     1561 tcattcccgt ccggagaaac gactgtgcag aggcgattct cgcgtgaggc ctcatcgaat
     1621 actttctgat cgtagcgcgg ggcgatgttc cgcagtttcg ggaagaccca gatctggaac
     1681 aatttcaccg ggtcgctttt cgacgcattc acttccgaat ggaaaattcc tgaacctgcg
     1741 ctcatgatct gcacttcgcc tttgcggatg acttccgtgc ggcccgtgct gtcgctgtgc
     1801 tgcaaagcgc cctcgagcgg aatggtaacg atctccatat tgtcgtgcgg gtgcttgccg
     1861 aaacccattc ccccggcgat cgtgtcgtca ttcagcacac ggagcagacc gaaatgaacg
     1921 agatccgggt cgtaccaccc ggcgaagctg aagctgtgat aggcgttaag ccagccgtga
     1981 tccgcatgac cacgaccgga agcgggaaat aattttatct gcatggcgaa tttgttttag
     2041 tgctgtttcc tgaacttatc gagcagatcg gaaactgtac gggcctcttc ttccgtcagc
     2101 acgcgaaaag tctcttcgtg ctgtgcgatc acaccgtcca gttcggaaag cagtttcagt
     2161 cctgccgggc tgatgagaat atctacctgc cggcggtcct tgggacaggt gttgcgctcc
     2221 accagctttt tatccacgag tttgtccaca agccttgacg cattggaact gcggtcgagc
     2281 atgcgctcct gtatcagtcc gaccgaggcc gggttgggat gctgtccgcg gagaatgcgc
     2341 aggacattgt actgctgtgt ggtaattccg tagggcttga aaaggcgctg ctggattgtc
     2401 tgcagccagg aagaagtgaa aaggatattc agtcccagct tctggtaaat gctggaaaat
     2461 tgtttttgtt tgatctcgtc ttcaagactc atgctaaggg agaatagata acctgtgctg
     2521 aagaaggaat tcttcgccga aattagggat tatccaatga cggcgttcgg ttattgctgg
     2581 aaaaaatgaa gctccgcgag gtctttcagc aggtaacgtt tcccgtcttt ttccgccgta
     2641 acaaatgcat cggtttgtcc tgcagcgatg atcttctggc gataagcttc cgcttccgcc
     2701 agcgtactga acgttcccat ggtgaaacgt gtaatgccgt cgtcgagcac tcttttgtcg
     2761 atcgaaccca ggggcagcag gcgtttgtac tggtaattgt ccgggaaatt ataggccgcc
     2821 acctgcacgt gaaaaagaag cccgggcgac ctggcattgc cgtacctggt cagaatatcc
     2881 gtgtagtcgt tcgcatttac ctggccttca ttcacaacgg aatctggagg cagttttttc
     2941 acaacagcaa cggttgacgt atcaacggtt ttcaggctgt cctgccgctt tttctctgca
     3001 tacgcttccg tgtagaaatg tatgtcgatc acagaacgct gcatctgtcc cgtctgcatg
     3061 gcgtcaagcg tccgtttctg gtgcaggctg tccaactcat acgtgacgtt gtaattcctg
     3121 cccagcggaa gcgtgatgag gtattttccc gtttcggaat tgctgctgac atgaaacatg
     3181 ctcagttttc ccgcatcgtc gtgaatgaaa acagaagcgt gcacaggctg atcgtcgagc
     3241 gtgatgaccc cgctgagcag catgacgttc gattggtcga gatgaaaatc gtaatcgacc
     3301 tggtaaatat cctgctgccc gtttccgccc ggttttccgc tggaataata accatgcttg
     3361 ccgtccgtag cgaggacaaa atatttatcg tcgcctgccg tattgatcgg gtagccgaca
     3421 ttcaccgggt cggaaggttc ccagaacgtg ctgtccaccg gcgtgaggtc cgtacggaaa
     3481 atgtcatacc cgcccatgct gttgtggccc tgtgagctga agatgagcgt ggttccgttc
     3541 gggtgaagga acggtgcgtc ctcatcataa ggcgtattta ttttgggccc catatttttc
     3601 acgttgcccc aagttccgtc gggctgcagc gtggcgacgt aaatatccct tcctccgtac
     3661 ccgccgggcc ggtcgctgga aaaatacatc gtatgcatat ccgtcgagaa cgttgcgctt
     3721 ccttcccagc tgtcggtatt cacgtcgcct ttcagcggga cgggtgccga ccagtaaaat
     3781 ccgttcagct ggctcgtata aatatcaccg ttgccttcct cgtccttgta aataagcagc
     3841 gtcgatccgt cgttggagat accgatgcat gcatcgtgcc cgttgccgtt gacggtggaa
     3901 tcgagcggct ccggttcgcc ccagttgttg ccgttgcgga acgacatgaa cacatcctcg
     3961 aaataaaaa
//
LOCUS       DASTSF010000205         3219 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_252304,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000205 DASTSF010000000
VERSION     DASTSF010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3219
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_252304"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..133)
                     /locus_tag="VFU15_05990"
     CDS             complement(<1..133)
                     /locus_tag="VFU15_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018341612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030527 - structural constituent of
                     chromatin [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEU4717359.1"
                     /translation="MTKAEVVNEIAEKTGIEKAAVQASVEAFMKVIKNSLSRGDNVYL
                     "
     gene            263..1315
                     /gene="mutY"
                     /locus_tag="VFU15_05995"
     CDS             263..1315
                     /gene="mutY"
                     /locus_tag="VFU15_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015481674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEU4717360.1"
                     /translation="MDFSRIIRSWYAKNRRDLPWRRTKDPYRVWLSEIILQQTRVAQG
                     LAYYHAFTGKFPDVKKLAAAKEQEVLKLWQGLGYYSRARNLHFSAKKIVKETGGQFPG
                     TYKDLLRLKGVGDYTAAAIASICFGEARPVVDGNVYRVLSRIFGIKTPIDSTEGKKIF
                     RETAAALIDHRDPGNFNQALMEFGALQCVPRNPDCAVCPFVQQCEARKKNTIALLPVK
                     AKKTKTRDRYFNYIVIRRGENVLLKKRTGNDIWKNMYDFPLVETEKPVSPEKLFSLEE
                     VKAITGNKKPVLTGRIHETKHVLSHQNIHARFFEVKAGTKRVAGEKLVACGRDDLHKY
                     PVPVIISRYLDNAWRE"
     gene            1408..1944
                     /locus_tag="VFU15_06000"
     CDS             1408..1944
                     /locus_tag="VFU15_06000"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEU4717361.1"
                     /translation="MAGVNKVILIGNLGKDPEVRYLEGGIAVANFPLATTETFRDKNG
                     NKNEQTEWHQVVLWRRLAEVAEKYLRKGQMVYLEGKIRSRSWEDKETGQKRYTTEIFG
                     DVLTILSRREDYQQNNGYQQQRPYGQQNQPYNNNNQQNAPVQQTQYPSQQQTFNPETN
                     TDPNHPAILPDSPDDLPF"
     gene            complement(1948..2409)
                     /locus_tag="VFU15_06005"
     CDS             complement(1948..2409)
                     /locus_tag="VFU15_06005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717362.1"
                     /translation="MRTRLLSFFVFAACISAHAHPSSLLEKLFRRSGNVIEARTLSVT
                     NTGSTSGYISLVVIEKTYKAHDLKTGDTIAVVMPGKFSGGSSEQGPPADVLLFLPAAS
                     SHVFASNGKNYRLFRPTDEWLGVHPYSRELELAVTDFANGRRHKKTGTDPV"
     gene            2474..3157
                     /locus_tag="VFU15_06010"
     CDS             2474..3157
                     /locus_tag="VFU15_06010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility lipoprotein GldD"
                     /protein_id="HEU4717363.1"
                     /translation="MRSLAWFCIFCGIVLSVSCGDDDDDALTPRPEGYFRIALPEKKY
                     VVYNAECPFSFEIPDYAHVYHSAAPMAEPCWRDLFFAPFKATVYISYKEITNDTLLEA
                     LVNQSWQLTEAHSQVASGLRDSLILRPDAHVYGDIQSLAGNAATQVQFYLTDSTKHFL
                     RGSLYFYCAPNKDSIAPVLDFVKKDIYHLAYTLQWNDRPLPVDEKRAVYTAGDSVIPV
                     DHKTPGNKK"
BASE COUNT          825 a          851 c          841 g          702 t
ORIGIN      
        1 gcaggtatac attgtcgccg cgggaaagag aattcttgat gactttcatg aatgcctcta
       61 cggaagcctg aacggcggcc ttttcaatcc cggtcttttc agcgatctcg ttaactactt
      121 ctgcttttgt catacttcta agcgatttac tatcaatggt ttaacatacc ctgatagggt
      181 tttacagggc tgcaaaatta cgctttttac tttgtctggc aaatttccgc ctaattttat
      241 cgccccaaca cgcagaattt caatggattt cagcagaatc atccggtcgt ggtacgccaa
      301 aaacaggcgg gatctgccct ggcggcggac caaagaccct taccgggtgt ggctttcgga
      361 aattattctc cagcaaaccc gggtcgcaca ggggctcgct tattaccacg catttaccgg
      421 gaaatttccc gacgttaaaa agctcgccgc tgcaaaagag caggaggtgc tgaagctctg
      481 gcagggactc gggtattatt cacgcgcccg caacctccat ttttcggcga agaagatcgt
      541 aaaggaaacg ggcggacaat ttcccggcac ttataaggat ctcctccggc tgaaaggtgt
      601 gggcgactat accgccgccg ccatcgcctc gatctgcttc ggcgaagccc ggccggtggt
      661 agatggcaat gtctaccgtg tgctgtcgcg catcttcggg ataaaaacac ccatcgattc
      721 gacggaagga aaaaaaatat tccgggaaac cgccgcggcc ctcatcgatc accgcgatcc
      781 gggaaatttc aaccaggccc tgatggagtt cggtgcgctg cagtgcgtgc cgcgtaaccc
      841 ggactgcgct gtttgtccgt tcgtgcagca gtgcgaagcc cggaagaaaa acacgatcgc
      901 gctgttgccg gtaaaggcga agaaaacaaa aacacgcgac cggtatttca attacatcgt
      961 catccggcgc ggagaaaatg tgctgctgaa aaaaaggacg gggaacgaca tctggaagaa
     1021 tatgtatgat ttcccacttg ttgaaacgga aaagccggtt tcgccggaaa agcttttttc
     1081 gctggaagaa gtgaaagcca tcaccgggaa taaaaagcct gtgctcaccg gccgtatcca
     1141 cgaaacgaaa cacgtgctga gccaccagaa tattcacgcg cgttttttcg aagtgaaggc
     1201 aggcacaaaa agagtcgccg gggaaaagct cgttgcgtgc ggccgggacg atcttcacaa
     1261 atatcctgtt ccggtgatca tcagccgtta cctggacaac gcgtggcggg agtgaatgcg
     1321 gataatttca ccgatcatta ttcttatctt tacgtttgaa acagataatc aactcaatac
     1381 aacagaaaca ccaatgtatt ttacgttatg gcaggagtaa acaaagttat tttgatcggg
     1441 aacctgggaa aagatccgga agtacgttac ctcgaaggcg gaatcgcagt tgctaatttt
     1501 cccctggcca ccaccgaaac attcagggac aagaacggga acaagaacga acagaccgaa
     1561 tggcaccagg tcgttctgtg gagaaggctc gcggaagtgg cggagaaata cctccgcaaa
     1621 ggacagatgg tttacctgga aggaaagatc cgcagccgca gctgggaaga caaagagacg
     1681 ggacagaaac gctacaccac ggaaattttc ggcgatgttc tcacgatcct ttcacgccgc
     1741 gaagattacc agcagaacaa cggctaccag cagcaacgtc cttacggcca gcagaaccag
     1801 ccttacaaca ataacaacca gcagaacgca cctgttcagc aaacgcaata cccgtcgcag
     1861 cagcaaacgt tcaaccctga aacgaacacc gatccgaatc acccggcgat attgccggat
     1921 tcaccggacg atcttccgtt ctgaatttta taccgggtcc gtcccggttt ttttgtgcct
     1981 tcttccgttt gcgaaatccg tgaccgccag ttccagctcc ctgctgtacg ggtgcacgcc
     2041 cagccattcg tccgtcgggc ggaaaaggcg ataatttttt ccgttggatg caaacacatg
     2101 cgatgaggcg gcaggaagaa aaagcagcac atccgcgggc ggtccctgct ccgaagaacc
     2161 accggaaaat tttcccggca taaccaccgc tatcgtgtca ccggttttca ggtcatgcgc
     2221 cttgtaggtt ttttcgatca ccacgagtga aatatatcct gacgtgctgc cggtattcgt
     2281 gacgctgagc gtccgcgctt cgatcacgtt tcccgagcgg cggaaaagct tttcaagcag
     2341 ggaggaagga tgcgcatgcg cggaaatgca ggcggcgaaa acgaaaaaag aaagcaggcg
     2401 ggtacgcata gcggatggtt tttacgcctg tccatgcaat tcgcgggccg gaatgtcagt
     2461 tatatttgca ggaatgagat cgctggcctg gttttgcatt ttttgcggga tcgtcctctc
     2521 cgtttcctgc ggggatgatg atgatgatgc gctaacgccg cggccggaag gttatttccg
     2581 cattgcattg cccgaaaaaa aatacgtcgt ctataatgcg gaatgcccgt tctcattcga
     2641 gataccggat tacgcccacg tctaccacag cgctgcgccc atggcggaac cctgctggcg
     2701 cgacctgttc tttgcgccgt tcaaagcaac cgtttacatc agctacaagg aaatcaccaa
     2761 tgatacattg ctggaagcgc tcgtgaacca gagctggcag cttacggaag cgcacagcca
     2821 ggtggcgagc ggtctccgtg attcgctcat cctgcggccc gatgcgcacg tgtacggcga
     2881 catacagtcg ctggcgggca acgcggccac gcaggtgcag ttttacctca ccgacagcac
     2941 gaagcatttc ctgcgcggat cgctttattt ttattgcgca ccgaataaag attccattgc
     3001 accggttctc gattttgtga aaaaagacat ctaccacctg gcgtatacgc tgcagtggaa
     3061 cgaccggccg ctgcccgtgg atgaaaaaag ggcggtgtac acggccggcg attccgtcat
     3121 tcctgttgat cacaagactc ccgggaataa aaaatgagcg atcccaaaga gaagttctcg
     3181 tcgtggaaat atttccgcaa gctctacgtg tccgcgctg
//
LOCUS       DASTSF010000206         7790 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_826219,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000206 DASTSF010000000
VERSION     DASTSF010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 7790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7790
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_826219"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1576)
                     /locus_tag="VFU15_06015"
     CDS             complement(<1..1576)
                     /locus_tag="VFU15_06015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717364.1"
                     /translation="MNSYNDNLHNTIVSSLLNQELEKKQIYSQANAAMFSLYHAEAAT
                     ITAQSKLTTATEDQTAKQAVNDQSVDATNISNNLVSAADQSNTYLKQAVTNTSVGAAN
                     VQIATNAIVKLAGDMGTVFSIVNAADFQSDIYDLTTDAYALINNTAQLAEVASDTAMN
                     TSIKTSEVPGSAVLTKAKNSNTALGNLYSITTSDFNNASAVVATDTVNVATASVAEEK
                     AQGTFENLSVEYRAAFSAYRTMNSELNLGLSTLHMPGMNDTSRNISFSLLRSPFPVLD
                     KTIKAPLGPVQTYYVMLVKDQSKTTFSIVNAEAIVLNENAKQFIKIDGTNYTNYLPSA
                     TEPQQKTSTKKSAKTGAAPEPGSTTNPTRFSFSFDFFNMDANTTEPTLMVDSDGDPIA
                     LGTDYVLFVMAVYTEAYKKKLNTFDDYLSAGSVKFTLTHKLEGVTATSITLGLNTQAE
                     TQAAHPFAFNFPMSGNAGYTVQYRCMLLPYGTEEVPTGMMNELSFLELQADLLRMQQE
                     ADSYDAQISGLNEKLAA"
     gene            complement(1689..5849)
                     /locus_tag="VFU15_06020"
     CDS             complement(1689..5849)
                     /locus_tag="VFU15_06020"
                     /inference="COORDINATES: protein motif:HMM:NF039901.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="contractile injection system tape measure
                     protein"
                     /protein_id="HEU4717365.1"
                     /translation="MQQTDRHIISSLAWQTSFDRKEKAPDLQDRLSRWTRHVLPKAMA
                     AVFDHLCPPGQTWRIQSLDLDLGEVDYNELESELFTRLKKILTEKLSDLISYSENSNS
                     SNLEIFSEETSRLGLLESFLLNGWLPWNHLPGESSVNQLMLQQLRSNRYETAAMIRRH
                     GSRHERVRKRIAYQFPDPVVQRIIEGLEPNNHAEVNGFTTEMIRMQEKDTVVQSSTAD
                     FRKQVWFWVLNYLLVERGTLYNRIAFLKSTLRQMSDHYNISYRELLALISEAVAVTKE
                     RTVIKSGFVRTLNIISEEFGAETARLAPAGTTTVDHWARLEILLQHSSERKSSSTRAE
                     FNDLVVSLSREDTHRFRELLRSAAKKNTRGIAEDLTDASAASLLTAVSPFAAAEITES
                     MHFLGLIFAEMKLRVTRSEWRSAALEFVLRAESASTPEKFTEWFVGKIIREKRIATRR
                     IVRRLHSVDIPAEAKTGRSPGIYSALLAALENNDPGKAHIAPAEEMKTLLTAFAQQLE
                     KPNARRNKLSALRQSLTHAVGTDPATALALLSSFTEKHAATRILDHVMNAGVMKKLAG
                     ALPQETSSFIASLLKTARKRKTREAAFLAEWMEFHLHALAVGAFVMYSGKSGAEFHAM
                     LIEEIFLQLHDGPANEFTLLLESIAGKSSVPALSAAAARWKTDHAPLPVSEQLGMLLS
                     AAERRPEKVARLLRNRFDDTQLTAWIASHRKQSEAVLAFLVHGGAQLMRTLVRKFEKM
                     LPREVKNSRTEKTLQLLFWKCAIDFEAHGGHRGKLEKSVETAVHAHFALRGGISAERK
                     NVRTASPRGKKTLHDGSGHSLSPAEMLQLIRKMLSEETETAEHGDAVVSLSELMKTVM
                     ESDPAALEKIFTLVPPSLKRAEILTASVSFREFIFHFIVHGKNTEVFEALRLPGAIAE
                     SVATGETARKLATGFWLRAWKLLRSGARGRTDVKKIIRESFAMLAKDGGIGGDVILEE
                     ILKKNTPVSPALMKTLEEMYPVFSDTAKETGHAKAGEWLLSGTLGSICSHVIRHREFP
                     AWCGIRDEKEERKLLHDILQHYPEQFFSVMMEEIIPEKSFSWLSRMIPFSTIADAIAW
                     LKPGYRSEMNILREFHAALGRMSFRGINAATLQDVIFRKTLLALASGNWRIISTSQVW
                     NELAWEICVHEGVPRNQFIRDVRRQENLLPPSLKIAFSYFAPEEKQAPVLPVAAKKAA
                     EKKAEPRKEPAKTLQGGIAVRNAGIVLLNNYIMMLFERLGIVKEKKFIHENARLDSVH
                     HLQYVVTGHSRTEESLLPLNKVMCGIPLDTPVREGIDIKPEEKNLTEGLLKAAIGHWP
                     VIGSTSVEGFRGNWLVRDGLLTERDDRWELTVEKKPYDILLHKSPYSFSIIKYPWMEK
                     PLHVTWPY"
     gene            complement(5836..>7790)
                     /locus_tag="VFU15_06025"
     CDS             complement(5836..>7790)
                     /locus_tag="VFU15_06025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717366.1"
                     /translation="PKGTYRDINNYYSIQNTFPEIFAVGPDSITANATDYQIAQSRQL
                     KGYLTLFDQVLANQFTQLACVPRLFSFKNSMTAAPFDRRTYFEKKTSSERRQEKFPAP
                     YLRFSPTYFCQSLYDIPNIKPLLKDNDTFDFSFGEEPQKVQERESWERYMLDPYNPYM
                     HGLMKFMESDSTSYERRNAILDHLLARHGESPYVINMLIANSVYAGETMKDRVVFKSM
                     YLQNLGLLTYNRAKGYNFLGADTLIRKDEQDISPPWKIPVPAHFEELIFTGETVDSVF
                     NSRRANWEEKLDEQDFINYSGIELKLSLLFGMKATYDDLLTAVLLNDDADQTKNFPQP
                     VEDGLSMSLWFITRRRGFIFLETLLLLQNFEYQVTLCTQQKTAGTWQCYNVSGTETYD
                     AVKLIIRTLIVQDNATLSGWVSDGKFSTCGMTFSITSGEDTNHYGQQIPGTDFSIVVT
                     SLPLQQITLCTQQKTEGTWYCYNISGPQTAATVQLVTATLQSAGNSMIAGWISAGEMT
                     AGDITYPISPAPDTGQYGEPVTGTHYNLLVTNDASPDASLALNYSCFDDDLQLIFPDY
                     IPQFAEPSFRLMTQLFMENELPEELSYGFYLAGFTSLQPLVTNFIYWHESLRYKSSYA
                     ANSAVYAKGVLSAVNSLITPVPNAAN"
BASE COUNT         1568 a         1956 c         2215 g         2051 t
ORIGIN      
        1 tggcggcgag cttctcattg agcccggaga tctgtgcatc gtatgaatcg gcttcctgct
       61 gcattctcag caggtcggcc tgcagttcaa ggaaagaaag ctcgttcatc ataccggtcg
      121 gaacttcctc tgttccgtac ggaagcagca tgcaccggta ttgaacggta taaccggcat
      181 tgcccgacat gggaaagttg aatgcgaacg gatgagctgc ctgggtttct gcctgggtat
      241 tcagaccgag ggtaatgctg gttgcggtta ctccttccag cttatgtgtc agcgtgaatt
      301 ttacggaacc ggcagacagg taatcatcga acgtgttcag cttcttcttg tacgcttccg
      361 tgtaaacggc cattacgaaa agaacataat ccgttccaag agcgattgga tctccatcgg
      421 aatcgaccat cagggtgggt tccgtggtgt tcgcatccat attgaagaag tcgaagctga
      481 aagagaacct cgtcgggttg gttgtgctgc ccggctccgg tgcagcgcct gtcttcgcgc
      541 tttttttcgt cgaggttttc tgctgcggtt cggtagccga aggcaggtaa ttcgtatagt
      601 tcgtgccgtc gatcttaatg aattgcttcg cattctcatt gagcacgatc gcttccgcat
      661 ttacaatgga gaaggttgtt ttcgactggt ccttcaccag cattacgtaa taagtctgca
      721 ccggtccgag cggagccttg atcgttttgt caaggacggg gaaaggactg cgaagaagac
      781 tgaagctgat gttgcgtgac gtgtcgttca tgccgggcat gtgcagcgtg ctgagtccga
      841 ggttgagttc ggagttcatc gttctatagg cggaaaaggc cgcgcggtat tcgacgctga
      901 ggttttcgaa tgttccctgc gccttctctt cggctacgct tgccgtggcg acgttcaccg
      961 tgtcggtggc aaccactgct gaagcgttgt tgaaatcgct cgtcgtgatc gaatagaggt
     1021 tgccgagcgc ggtgttcgag tttttcgctt tcgtgagcac ggcggatccc ggaacttctg
     1081 aagttttgat cgacgtgttc atcgccgtgt cagaagcgac ttcagcaagc tgcgctgtat
     1141 tgttgatcag cgcgtaggcg tccgtggtga gatcataaat gtccgactgg aagtcggctg
     1201 cattcacgat gctgaaaacg gtgcccatgt cgccggcgag cttgacgatg gcgttcgtcg
     1261 cgatctgaac gttcgcagct ccgaccgatg tgttcgtcac ggcctgcttg aggtacgtgt
     1321 tcgattggtc ggcggcgctg acgagattgt tggaaatgtt ggtggcgtcg accgactgat
     1381 cgttgacggc ctgcttggcg gtctggtctt cggttgcagt cgtcagtttg ctctgggccg
     1441 tgatggtcgc tgcttccgca tggtacagcg aaaacatcgc ggcgttggcc tggctgtaga
     1501 tctgtttttt ctccagctcc tggttcagca gcgacgacac gatggtgttg tgaaggttgt
     1561 cgttgtaaga attcatgtgg tttgatttgg tggtgaatga ttagttgatt gtttttgatc
     1621 ggttgggagg aaagcgcgtc agctgctgtc cggttttttc ggttcgttgt tttttttcat
     1681 acgttttttc aataaggcca tgtaacgtga agcggttttt ccatccacgg gtattttatg
     1741 atcgagaaag aataaggcga tttgtgcagg agaatgtcgt acggtttttt ctctaccgtc
     1801 agctcccacc ggtcgtcgcg ttccgtcagc agcccgtcgc gcacgagcca gtttccgcgg
     1861 aacccttcga cggatgtgct gccgatcacg ggccagtgcc cgatcgctgc tttcagcagt
     1921 ccttcggtaa ggtttttttc ttcgggcttg atgtcgatgc cttcgcgcac cggcgtgtcc
     1981 agcggaatgc cgcacatcac tttattcagc ggcagcagcg attcttccgt gcggctgtgt
     2041 cctgtcacca cgtattgcag gtgatgaacg gaatcgaggc gggcgttttc atgaatgaat
     2101 ttcttttcct tcacgatgcc gagacgttcg aacagcatca tgatgtaatt gttcagcaat
     2161 acgatgcctg cgttacgcac tgcaataccg ccctgtaaag tcttggcagg ctctttccgt
     2221 ggttctgctt tcttttcggc cgcttttttt gctgccaccg gaagaacagg tgcctgcttt
     2281 tcttcaggag cgaaatagct gaacgcgatt ttcagcgacg ggggaagcag gttttcctgc
     2341 ctgcggacgt cgcgtatgaa ctggttgcgc ggaacgcctt cgtgcacaca gatctcccat
     2401 gccagctcgt tccatacctg gctggttgaa atgatccgcc agttcccgct tgccagcgca
     2461 agaagggtct tcctgaaaat cacgtcctgc agcgtcgccg cgtttattcc gcggaaactc
     2521 atccggccga gcgctgcgtg aaattccctg agaatgttca tctccgaacg gtatcccggc
     2581 ttcagccagg cgatcgcatc ggcgatcgtg ctgaacggga tcatgcggga aagccaggaa
     2641 aaactttttt ccgggatgat ctcttccatc ataaccgaga agaattgttc gggataatgc
     2701 tgcagaatgt catggaggag tttccgttct tctttttcgt cgcgaatacc gcaccacgcg
     2761 ggaaattcgc ggtgccggat aacgtgcgag cagatgctgc cgagcgttcc gctcaggagc
     2821 cactccccgg cttttgcgtg gcccgtttct tttgcggtgt cagagaacac cgggtacatt
     2881 tcttcaagcg ttttcatgag cgccggtgag acgggcgtgt tttttttcag gatctcttcg
     2941 aggataacgt cgccgccgat gccaccgtct tttgcgagca ttgcgaacga ttcgcggatg
     3001 atctttttta catcggtccg ccccctcgcc ccggaacgga gcagcttcca tgcgcgcagc
     3061 cagaagccgg tcgccagctt ccgtgcggtt tctcctgtcg caaccgattc tgcgatagcg
     3121 cccggaagcc gcagggcttc gaatacctct gtgttctttc cgtgcacgat aaaatgaaag
     3181 atgaattccc ggaacgaaac agaggcggtg agaatttccg cacgtttcag cgacggcggc
     3241 acaagggtga atattttttc cagcgcagcc gggtcgcttt ccataacggt tttcatcagc
     3301 tcgctgagcg aaacgacagc atctccgtgt tccgccgttt cggtttcttc gctcagcatt
     3361 ttccggatga gctgaagcat ttcggccggg gaaagggaat gaccgcttcc gtcgtgaagt
     3421 gttttttttc cgcgtgggga ggcagtacgc acgtttttcc tttcggcgga aattcctccg
     3481 cgtaacgcaa aatgtgcgtg cacggctgtt tcgactgatt tttccagttt cccccgatgg
     3541 ccgccatgtg cttcaaaatc gatcgcgcat ttccagaaaa gcaattgaag ggttttttct
     3601 gttcgggaat tttttacttc gcgtggaagc attttctcaa acttccggac aagcgtccgc
     3661 atgagctgtg cgccaccgtg cacaaggaat gcaagtaccg cttcgctttg tttcctgtga
     3721 ctggcaatcc atgccgtcag ttgtgtatcg tcgaagcggt tgcgcaatag tcttgcaact
     3781 ttttcaggcc ggcgttctgc cgctgacagc agcatgccga gctgttcgct tacgggaagg
     3841 ggcgcgtgat ctgttttcca tcgtgcagct gctgcggaca aggcgggcac ggaagatttc
     3901 ccggcgatac tttcgaggag cagtgtgaac tcgttcgcgg gcccgtcatg aagctgaaga
     3961 aaaatttctt cgatcagcat tgcatggaat tctgctccgc ttttgccgct gtacataaca
     4021 aatgcaccga cggccagcgc gtgaagatga aactccatcc attctgccag gaatgcggct
     4081 tcccgcgttt tccgcttccg cgctgttttc aggagcgaag cgataaagga agaagtttcc
     4141 tgcggcaagg cgccggcaag ctttttcatt acgcctgcat tcattacgtg gtccaggatg
     4201 cgggtcgctg catgtttctc cgtgaatgaa gaaagcaatg cgagtgcggt tgcagggtcc
     4261 gtcccgactg cgtgcgtgag cgattgccgg agcgcggata acttgtttcg tcgtgcattg
     4321 ggcttttcca gctgctgcgc gaatgcggtg agcagcgtct tcatttcctc agccggtgca
     4381 atgtgtgctt tcccgggatc gttgttttca agcgcggcaa gaagcgctga ataaattccg
     4441 ggcgaccggc ctgtttttgc ttcggcagga atatccaccg aatgaaggcg gcggacgatc
     4501 ctgcgggtag cgatgcgttt ttcgcggatt atttttccga cgaaccattc cgtgaatttt
     4561 tccggcgtgg aagcgctttc ggcacggagc acgaattcca gcgcggcgct tctccattcg
     4621 ctgcgcgtta ccctcagctt catttccgcg aagatcagcc cgaggaaatg catgctttcc
     4681 gttatttcgg cggcggcaaa aggactgacc gcggtgagca gagatgcggc ggatgcgtcg
     4741 gtgagatctt cggcaatgcc ccgggtgttt ttcttcgcgg cggaacgcag caattccctg
     4801 aagcggtgtg tatcttcccg ggaaagactt acgacgaggt cattgaattc cgcgcgggtg
     4861 gaagaggatt tgcgttccga tgaatgctgc aggaggatct caagacgtgc ccagtgatca
     4921 acagttgttg tacccgcggg agcaagccgt gcagtctccg ctccgaattc ttcagaaatg
     4981 atattcagcg tccggacaaa accgctcttt attactgttc tttctttggt gacagctacc
     5041 gcttcgctga tcagcgcgag cagctcacga taagaaatgt tgtaatgatc ggacatctgc
     5101 cgcagtgtgc ttttcaggaa agcgatgcga ttgtacagcg tgccgcgttc cacgagcagg
     5161 taattcagca cccagaacca cacctgtttc ctgaaatcgg cagtggacga ctgcacaacc
     5221 gtgtcttttt cctgcatacg gatcatctcg gtcgtgaagc cgtttacttc cgcgtgatta
     5281 ttcggttcga gaccttcgat gatgcgctgt acgaccgggt cggggaactg gtaagcgatg
     5341 cgtttgcgca cccgttcatg gcgggacccg tgacggcgta tcatcgccgc tgtttcgtaa
     5401 cggttgctgc ggagctgctg cagcatcagc tggtttacgg agctttcccc ggggagatga
     5461 ttccacggca gccacccgtt cagcaggaag ctttccagga gaccgagacg ggaagtctct
     5521 tcgctgaaaa tttcgagatt ggaactgttg ctgttttcgg aataggagat aagatcggac
     5581 agcttttccg taagtatttt tttcagtcgc gtgaaaagct ccgactcgag ctcattataa
     5641 tcgacttcgc cgagatcgag atcgagcgat tgtattctcc acgtctggcc cggcgggcaa
     5701 aggtgatcga aaacagcagc catcgctttc gggagcacgt gccgtgtcca gcgacttagg
     5761 cgatcctgca ggtcaggcgc tttctccttc cggtcgaaag aagtctgcca tgcaaggctg
     5821 gagatgatat gcctgtcagt ttgctgcatt cggaaccggt gtaatcagtg agttgacagc
     5881 ggaaagaact ccttttgcat aaacagcgga gttggcggca tagctgcttt tataccgtag
     5941 gctttcgtgc cagtaaatga aatttgtcac cagcggttgc agcgaagtga aaccggcgag
     6001 gtaaaaaccg tacgacagct cttcaggcaa ttcgttctcc atgaagagtt gtgtcatcaa
     6061 ccggaacgat ggttccgcga attgcggaat ataatccggg aagatcagtt gaaggtcgtc
     6121 gtcgaagcat gaataattga gcgcgagcga tgcatccggt gaagcgtcgt tcgttacaag
     6181 gaggttgtaa tgcgttcccg tgaccggttc gccgtactgg cctgtgtccg gcgcagggct
     6241 gatcggatag gtaatatcac ctgcggtcat ttcacccgcg ctgatccacc ctgcgatcat
     6301 gctgttaccg gccgactgaa gagtggcagt aaccagttgg acagtagctg ccgtttgcgg
     6361 acctgaaatg ttgtaacaat accatgtgcc ttccgttttt tgctgcgtgc acagcgtgat
     6421 ttgctgcagc ggaaggctgg tgacgacaat gctgaaatcc gttcccggga tctgctgccc
     6481 gtaatgattg gtgtcttccc cggatgtgat ggagaacgtc atgccgcagg tgctgaattt
     6541 tccgtcgctc acccatccgg aaagtgtcgc gttgtcctgt actatcaatg tgcggatgat
     6601 cagcttcacc gcgtcatagg tttctgttcc cgatacattg tagcattgcc acgttccggc
     6661 ggtcttttgc tgcgtgcaga gcgtcacctg gtattcgaaa ttctgaagaa gaagaagcgt
     6721 ttcgaggaaa atgaacccgc gccggcgcgt aatgaaccag agcgacatgc tcagtccgtc
     6781 ctcaacgggc tgcgggaaat tttttgtttg atctgcatcg tcattgagaa gaaccgccgt
     6841 gaggagatcg tcgtaggttg ctttcatccc gaagagcagg ctaagcttca gctcaatacc
     6901 ggaatagttg atgaaatcct gctcgtcgag tttttcctcc cagttcgcac gacgggaatt
     6961 aaaaacggaa tcaaccgttt cgccggtgaa gatcagttct tcaaaatgcg caggaaccgg
     7021 aattttccac ggtggagaaa tgtcctgctc atctttccgg atgagcgtat ccgcaccgag
     7081 gaagttgtat cctttggcgc ggttatatgt cagcagcccg aggttctgca ggtacatgct
     7141 tttgaacacc accctgtcct tcatcgtttc acctgcgtaa accgaattgg cgatgagcat
     7201 attgatcacg tagggcgatt ccccgtgacg ggcaagcagg tgatcgagga ttgcgttcct
     7261 tctttcatag ctcgtgctgt cgctctccat gaatttcatc agcccgtgca tgtacgggtt
     7321 gtacggatcg agcatgtagc gttcccagct ttcacgctcc tgtacttttt gcggctcttc
     7381 cccgaaggag aaatcgaacg tatcgttgtc tttcaggaga ggcttgatgt tcggtatgtc
     7441 gtaaagcgac tggcagaaat aggtcggtga aaaacggaga taaggagcgg gaaatttttc
     7501 ctgcctgcgt tcgctgcttg ttttcttttc gaaataagtg cgcctgtcga acggagccgc
     7561 cgtcattgaa tttttgaagg agaaaagacg cggcacgcac gccagctgcg tgaactggtt
     7621 cgcaagcacc tggtcgaaaa gcgtgagata gcctttcagc tggcgcgact gtgcgatctg
     7681 gtaatccgtg gcgttagcgg tgatcgaatc cgggccgacc gcgaatattt cggggaacgt
     7741 attctggata gaatagtaat tgttgatgtc gcggtaagtt cctttcggaa
//
LOCUS       DASTSF010000207         4449 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1898902,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000207 DASTSF010000000
VERSION     DASTSF010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4449
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1898902"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..238)
                     /locus_tag="VFU15_06030"
     CDS             complement(<1..238)
                     /locus_tag="VFU15_06030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717367.1"
                     /translation="MITLRISKRTSRYVAMTFAMIMFSDMACPLAAWALTGGPSQPEV
                     QSFEPVSTSDMVDLFSGDFNYNIPLLDMDGYPINI"
     gene            complement(252..896)
                     /locus_tag="VFU15_06035"
     CDS             complement(252..896)
                     /locus_tag="VFU15_06035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717368.1"
                     /translation="MKMKARYIFLLLLFASCKWNGQHPGRAFTDVSGKTLSPADYSRW
                     FESDESTQQHLQQAGGKSYEVTFLPADLLALHEAGPGADENAIREAKTHYEDLVYFRL
                     RIKDPDAGGEILKENISSPQDYQQRVTYCAFGMQRDISLLDEQNDTIPCALFNFERAY
                     DVTPFVNFVCAFDRKEVGQCRFFTFVFNDRLFHNGFLKFSFTQEDLRNTPKLKP"
     gene            complement(893..1423)
                     /locus_tag="VFU15_06040"
     CDS             complement(893..1423)
                     /locus_tag="VFU15_06040"
                     /inference="COORDINATES: protein motif:HMM:NF015871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HEU4717369.1"
                     /translation="MWNKSVTVIVSLALGLCGTGMGLYSKMSGPKYGYVKIKDVFDRF
                     DMKKEMETKYQKDNGWKQRELDSMAFVLQGEGNRLKSMGEVPDSLARDFSLRQNDFLA
                     RRKQLEDEKQSQTSEYNQQIINRMNQYINEYGQHNGYDFILGDDGNGNVMFASPARDI
                     TEPIIRYINDRYSGKQ"
     gene            complement(1413..2513)
                     /locus_tag="VFU15_06045"
     CDS             complement(1413..2513)
                     /locus_tag="VFU15_06045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717370.1"
                     /translation="MKKIFLFISLAAGLAARAQVTVVSANLIAYNVTPVSICQVTLSD
                     PQGDQDVILDAAVYNSSGEALLEVRTMPVHVHMGQNVIQSQSVMIATATYSASPQGNF
                     LQAQKQLPSGVFRHCVRVLHAGGESDDEFCEEVESDDNSFLSLVSPFDKDTIESKTPV
                     LTWTHSEPFNLLSPGESFKLVLVEQKTDQTPEAAVLANQPYYQFFNLLRHDVQYPFDA
                     PALKEGMSYAWQVQKLSGDGTVINKTEAWQFTILKPVVQKENKYCVLKKTLDAGYYTA
                     GNNKIFFRFDEPYSGNKLKCAIYDEAHHRINPQTDNEKKKDGDQPAVNVKSNGYNLFE
                     IDLDPLGLKPGFYFLEVYTEKNEKYTLKFYVE"
     gene            complement(2530..>4449)
                     /locus_tag="VFU15_06050"
     CDS             complement(2530..>4449)
                     /locus_tag="VFU15_06050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717371.1"
                     /translation="VKNVPLSVTGYYTTLQNAGGLKNSVRVSFDAQAYRQHLRQNCRV
                     STGRLQDSLAGLAHVTQKYQQHLLYLKYLQSLSPEQYRKQLDLSHQRAYEDSLKSAHA
                     HLPDSVKAEIPSTTVLPSVPVFRLPSGEHYKDSLESLITRQQNLIDSVSSVVAVLKKQ
                     IAHTDSLLNDVRTPSASLPGNPFLSKVQTLSIGLCYPSFSPFLLSGVPLRGCQVEMQS
                     GKNYFAAAAGKTVSNLMLSPDLIRNNLNSVRNLYNFFDFNQLASGRSIAAVKAGRGTK
                     EGTHLFAGLLFGKGLESYWPDSVSSMLNTGRQEKNLVADIDAKFVFSPSQSISIDYAK
                     SVILPALSHSEDSVSQNVKWLDYHYRSHAVLAKYEGAFPKLRSSVTVSFRLVDPYFRS
                     FGAGFVRQDNIRYEIRTQHQVSKKLQLSFSLRREANNLLRLYDQFTVLQMAGVSASLK
                     LTRNMQLRASYNPVFQQVKENGQIIYTNNNQIAIASVNYKLHGRKWNSLFTGLYSYYK
                     LYDGNENRTYSNVLASFMSQCRKGWTFALQGAGYSVSPSDTLFSNSIVCTAAAGHKWK
                     NGLQLQGNAEYAFTSLYGNHAGYGLDVEIPLYKTTALSLTAKKIAPGDFYNSFSSQVY
                     KRFPCFCSAQLIVNW"
BASE COUNT         1007 a         1115 c         1200 g         1127 t
ORIGIN      
        1 cgatgttgat cgggtaaccg tccatgtcca gcaacgggat gttgtaattg aagtcgccgg
       61 aaaaaagatc gaccatgtcg gaagtcgata ccggttcgaa gctctgcact tccggttggc
      121 tcggtccgcc cgtaagcgcc catgccgcga gcggacatgc catatctgaa aacatgatca
      181 tggcgaatgt cattgcgaca tacctggatg ttcttttgct gatgcgaagt gttatcatgg
      241 ctgcaggtga ttcagggttt cagtttcggt gtgttccgca ggtcttcctg cgtgaatgag
      301 aatttcaaaa agccgttatg gaaaagccgg tcgttgaaaa cgaatgtgaa aaaccggcac
      361 tgcccgactt ctttccggtc gaaggcgcag acgaaattca cgaaaggagt tacgtcgtac
      421 gcgcgttcga aattgaacag tgcgcaggga atggtgtcgt tctgctcatc aagcagcgag
      481 atatcccgct gcataccgaa ggcgcaatac gtcacacgct gctggtagtc ctgcggcgaa
      541 gaaatatttt ccttcagtat ttccccgcct gcgtccgggt ccttgattct caaacggaaa
      601 taaacgaggt cttcgtaatg tgtttttgct tcacggatcg cattttcgtc agcgcccggc
      661 cctgcttcat gcagcgcgag aagatctgcg ggaaggaagg tcacttcgta gctttttccg
      721 cccgcctgtt gcaggtgctg ttgcgtgctc tcatccgatt cgaaccaccg ggagtagtcg
      781 gcgggcgaca gcgttttccc ggaaacatcg gtgaacgcgc gacccggatg ctggccgttc
      841 catttgcagg atgcgaacag gagcaggaga aaaatataac gggctttcat tttcattgct
      901 tgcctgaata acgatcgttg atgtagcgga taatgggttc ggtaatgtcg cgcgcgggcg
      961 aagcgaacat gacattgccg ttcccgtcgt ccccgagaat gaaatcatac ccgttgtgtt
     1021 gcccgtactc gtttatgtac tggttcatcc ggttgatgat ctgctggttg tattccgatg
     1081 tttgcgattg tttctcgtct tccagctgtt tccgccgggc gaggaaatca ttctgccgca
     1141 gcgaaaaatc acgcgcaagg ctgtccggca cttcccccat cgatttcagc ctgttccctt
     1201 cgccctgcag gacgaacgcc atgctgtcca gctcccgctg cttccagccg ttgtctttct
     1261 ggtatttggt ttccatttct tttttcatgt cgaagcggtc gaagacgtct ttgatcttca
     1321 cataaccgta tttcggtccc gacattttcg agtagagacc cattcccgtt ccgcacaggc
     1381 ccagcgccag cgaaacaatc accgttaccg atttattcca cataaaactt cagtgtgtat
     1441 ttttcgtttt tttccgtgta tacttccagg aaataaaagc ccggcttcaa cccgagcgga
     1501 tcgaggtcga tctcgaagag gttgtaaccg ttgctcttca catttaccgc aggctgatca
     1561 ccatcctttt tcttttcgtt atctgtctgc gggttgatcc ggtgatgcgc ctcatcgtaa
     1621 atggcgcatt ttaatttgtt tcccgaatac ggctcgtcga aacggaagaa gatcttattg
     1681 ttgcccgccg tgtagtaacc ggcgtccagc gtttttttca gtacacagta tttgttctct
     1741 ttctgcacaa cgggtttcag gatggtgaac tgccacgctt ccgttttgtt gatcaccgtt
     1801 ccgtcgcccg agagtttctg cacctgccag gcatagctca tcccttcttt cagcgcgggt
     1861 gcgtcgaacg gatactgcac atcgtgccgc agcaagttga aaaactggta atacggctgg
     1921 ttcgccagga cagctgcttc aggcgtctgg tcggttttct gttcgacgag cacgagcttg
     1981 aaggattccc cgggcgaaag caggttgaaa ggttcggaat gcgtccaggt cagcacgggc
     2041 gtttttgatt cgatggtgtc tttgtcgaac ggagaaacaa ggctgaggaa cgaattgtca
     2101 tccgattcaa cctcctcgca aaattcatcg tcagattcgc ctcccgcgtg cagcacgcgt
     2161 acgcaatggc ggaacacgcc cgacggcaat tgcttctgcg cctgcaggaa attcccctgc
     2221 ggggaggcgg agtacgtagc cgtggcgatc atcaccgact gcgactggat cacgttctgt
     2281 cccatgtgaa cgtgaacggg catggtccgt acttcgagca gggcttcacc ggaagaattg
     2341 tagacggccg cgtcgaggat gacatcctgg tcgccctgcg ggtcagacaa tgtcacctgg
     2401 cagatagata caggcgttac gttgtatgca ataagattgg cggaaacaac cgttacctgt
     2461 gcccgtgccg caagaccggc ggcgaggctg atgaagagga atattttttt catgttcatg
     2521 attttccgtt taccagttga cgatgagctg cgccgagcag aaacagggaa agcgtttata
     2581 tacttgcgag gaaaagctgt tatagaaatc ccccggcgcg atttttttag ccgtaagcga
     2641 aagggcggtc gttttataca gcggtatttc cacgtcgaga ccgtatcccg catgatttcc
     2701 gtacagcgaa gtgaatgcat attccgcgtt tccctgcagc tgcaaaccgt ttttccattt
     2761 gtgcccggca gcagccgtac aaacaatgct gttgctgaaa agtgtatccg acggggagac
     2821 ggagtagccc gctccctgta aagcaaacgt ccagcctttc cggcattgcg acatgaatga
     2881 agcgagcaca ttggaatagg tcctgttttc attaccgtcg tacagtttgt aatagctgta
     2941 cagcccggtg aaaagcgaat tccattttct tccgtgcagt ttataattca ccgatgcgat
     3001 cgcgatctgg ttgttattgg tataaataat ctgcccgttc tcttttacct gctggaaaac
     3061 gggattgtag gaagcgcgca gctgcatatt tcgtgtgagc ttcaggctgg cggaaacacc
     3121 cgccatctgc aggacagtga attgatcata cagacggagg aggttgttcg cttcccgccg
     3181 gaggctgaag ctcagctgga gctttttgga aacctggtgc tgcgtcctga tctcgtagcg
     3241 tatattatcc tgcctgacga aaccggcgcc gaacgaacgg aaatacggat cgacgaggcg
     3301 gaaagaaacc gtaacggaag aacgtagttt cgggaacgct ccttcgtatt tcgcaagcac
     3361 ggcatgtgaa cggtaatggt aatccagcca tttcacattt tgcgaaacgc tgtcttccga
     3421 atgggacagt gcaggaagaa tgaccgactt ggcgtaatca atggaaatac tttgcgaagg
     3481 agaaaaaaca aatttcgcat cgatatcggc tacgaggttt ttttcctgcc tgccggtatt
     3541 cagcattgat gatacgctgt cgggccaata cgattcgagc cctttcccga aaagcagccc
     3601 ggcaaaaaga tgcgtgcctt ctttcgttcc tcttcccgct tttaccgccg caatgcttct
     3661 tccggatgca agctggttga aatcgaaaaa attatagagg ttacggacgc tgttcaggtt
     3721 gttccggatc aggtccggcg aaagcatcag gttgcttacc gtttttcctg ctgccgctgc
     3781 gaaatagttt ttcccgcttt gcatctcgac ctggcagccg cgtaacggaa caccggaaag
     3841 aaggaaaggg gaaaaagaag gatagcacaa accgatgctc aacgtttgca ctttcgacaa
     3901 aaaggggttg cccggcaggg aggccgaagg agtgcgcaca tcattgagca aactgtcggt
     3961 atgtgcgatt tgttttttca gcaccgcgac gacggatgaa accgaatcga taaggttctg
     4021 ctggcgcgtg atcagcgatt ccaggctgtc tttataatgc tcgccggagg gaagccggaa
     4081 aaccgggact gaaggcaaaa cggtagtgga aggaatctct gccttcacgg aatcgggcag
     4141 atgcgcatgc gctgatttga gggaatcttc gtacgcccgc tggtgtgaaa gatcgagctg
     4201 cttccggtac tgttccggtg acagcgactg caggtacttc aggtaaagaa gatgctgctg
     4261 gtatttctgc gtgacatgag cgagacccgc gaggctgtcc tgcaacctgc cggtggaaac
     4321 gcggcaattc tgccggaggt gttgacgata cgcctgcgca tcgaatgaaa cgcggacgga
     4381 atttttcagt cccccggcat tctgaagcgt cgtataatac cctgtgacgg aaagaggaac
     4441 gtttttcac
//
LOCUS       DASTSF010000208        11833 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_936275,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000208 DASTSF010000000
VERSION     DASTSF010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 11833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11833
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_936275"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..333
                     /locus_tag="VFU15_06055"
     CDS             <1..333
                     /locus_tag="VFU15_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide synthetase C
                     domain-containing protein"
                     /protein_id="HEU4717372.1"
                     /translation="SSFDLRIDPRTATTVMMVAGGYPGDYEKGKIISGLENVKDAIAF
                     HAGTRSANGNVVTNGGRVIAFTAFGNSIGAALKNSYAAASAVKFDGCYFRRDIGQDLL
                     KIAESAAK"
     gene            complement(390..581)
                     /locus_tag="VFU15_06060"
     CDS             complement(390..581)
                     /locus_tag="VFU15_06060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717373.1"
                     /translation="MNQEGFWYAFGRVCYKYFDGLQWTYDHLSPNKILITIGFISFAW
                     WIAWQNKFNQKQLREGGLK"
     gene            661..1659
                     /locus_tag="VFU15_06065"
     CDS             661..1659
                     /locus_tag="VFU15_06065"
                     /inference="COORDINATES: protein motif:HMM:NF040411.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6427 family protein"
                     /protein_id="HEU4717374.1"
                     /translation="MFIRNARSHRPVSLIFIPLIAVGLWLPGFLHPAPPAPMIFMPAF
                     GSVASWTSAWPVLNVFLGLAFTLLQAFLLNYIAQQQQLFTRKTWLPALMFVTFASCTP
                     SLLTFNPELVAGLFLLGALYVLLSTYRMDRAHSQVFNAGFLIGLAGIFYLPSLVFFIF
                     AIIAIIVLRPFVWREWAILFAGLLVPWIYSGVYFFWNDRIVSVTMENIYNPVVQRDFF
                     FKLPVEYYALAFMTGMVLLTSAGRFVAGAGTSTMKTKKGVSVMVWFLVFSIIAVIPAQ
                     NFAVPVFLFTVYPLSVFTSNYFLNARRQWLADTIYLFFLASIAATYFIKVSGLFGS"
     gene            1691..2383
                     /gene="purQ"
                     /locus_tag="VFU15_06070"
     CDS             1691..2383
                     /gene="purQ"
                     /locus_tag="VFU15_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008507456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HEU4717375.1"
                     /translation="MKFGVVIFPGSNCDQDMIYVLGKIMGQEVVELWHKDHDLQQVDA
                     VVLPGGFSYGDYLRSGAIARFSPIMKEVIAFAEKGGIVFGVCNGFQILCEAGLLPGAL
                     LQNNERKFICKNVFIRAQNNSTLLTQAVPQDKALKIPIAHGEGRYFASPEVLKQLNDN
                     GQVLFRYCDENGNVNTAANPNGAEENIAGICNAGKNVFGMMPHPERAADPELGNTDGK
                     FLFQSIVSLVNA"
     gene            2577..5294
                     /locus_tag="VFU15_06075"
     CDS             2577..5294
                     /locus_tag="VFU15_06075"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEU4717376.1"
                     /translation="MKKITLLLSAACIAVFSMAQQPQINPPSQPQIGTPQWAAQHAQD
                     PIERLAGPVYSPQSGNDTLNNEYTNSACGLNYSLGTVRLGQRFSPVGLPQPAGIAVNI
                     PPCATVLKAYLYTEALGVAPSITADLTDPANTTTSYNMSLIGNSIDVCWGMNGTHVWR
                     SDVTASISGPGMYSISGLPVSSTASSSTVDVEGATLLVIYADPYASYTGSIVIDDGCH
                     TVAGGTLTHTMTGFSACANSTNASSFMLVGDMQMMGYTISMNGNNVPQPQWDWWNQIS
                     ASTSVTSGQNTCTFQMNDMGDCYTLAVAGLYYQTPCQTCTPMATNILLSTSSTPETCN
                     GNGTASVTASGGTGNYSYTWLPGGSTSSTATGLTAGTYTVYVQDGTSCASATVNVGYN
                     GMVLSVTASPISCSSQGSATVNVTGGTGPFSYAWSPSGGTSATASNLAAGTYTVAVTD
                     QSNGCVMTATCTIVNNSTLTAYVYTTPDSTCSGNGGSATAYGYGGTQPYTYLWQPGGQ
                     TTQTITGLAQGSYTCTITDSAGCSATASDSVAAYSLWVYTGGYGMYSCGDSTQLYAMC
                     SDPSATFSWSPPTGLSNPNVQDPYLTVSGNITYTVTATSGCGTATDTFMVVVDSLNMY
                     NEQICFVTVDTATNKNKVIWERWNSPMTGYYNIYRETAVSGQYALVGTQPVSQFTTFI
                     DMSSNPVVMANRYVLTTVDGCGNESDTSYHHRTIHLQTSPGFSGAVNLQWTAYEGLPI
                     STYNIFRGPSMSQLTLLTQVAGNVFTYTDVTAPAGPLYYMVEAVHPYGGCSPSRLSQN
                     ATDYVGSQSNIDFFNPEGMASPDLQNSLMLSPNPGDGSFRLSVYLGQAQNVQTTIYDN
                     LGRVVYASSQDAAAGTFTSDIDLRGTSAGMYFVEVKTENGTAVKQLVIQ"
     gene            complement(5349..5738)
                     /locus_tag="VFU15_06080"
     CDS             complement(5349..5738)
                     /locus_tag="VFU15_06080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS/SEC14 domain-containing protein"
                     /protein_id="HEU4717377.1"
                     /translation="MNTLLPKTVQHRTGEITLEGTYYIYRISEGAEIVLEDAEELIRI
                     GTEMVGNRQVVSIVDGRNNFSITGEAREYFARQTDKNRFLAVAIITNSTAQRLIINFF
                     IRVNKPGVPTKLFANETEAKKWLQNFL"
     gene            complement(5735..6310)
                     /gene="nadD"
                     /locus_tag="VFU15_06085"
     CDS             complement(5735..6310)
                     /gene="nadD"
                     /locus_tag="VFU15_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012022833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HEU4717378.1"
                     /translation="MKTGLFFGSFNPIHVGHLVLANYMLAFTDLSEIWFVVSPHNPLK
                     EKKGLLQDNHRLRMVRMAIEDHPKMKASNIEFSLPQPSYTVTTLAHLAEKYPAKKFCL
                     IMGADNLASLRKWKNYEFILEHHSIYVYPRPDSDGGEFATHGSVIMTEAPLMEISSTF
                     LRNAIRNKKDVRYFFPPQVWEYLKEMHFYEK"
     gene            complement(6307..6888)
                     /gene="gmk"
                     /locus_tag="VFU15_06090"
     CDS             complement(6307..6888)
                     /gene="gmk"
                     /locus_tag="VFU15_06090"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEU4717379.1"
                     /translation="MQEKGKLVIFSAPSGAGKTTIVHHLLGRFPSLEFSVSATSRAPR
                     GEEQHGVDYYFLSADEFRRRVDAGEFVEWEEVYPGQYYGTLHSEVERIWRKGHTVIFD
                     VDVTGGLNLKKKFGGAALSVFVRPPSVEALEKRLRQRATEPEEKIRMRVSKAVHELTF
                     EPSFDRVIVNEDLQKAFAEADGIVGGFIEKKQH"
     gene            complement(6942..7808)
                     /locus_tag="VFU15_06095"
     CDS             complement(6942..7808)
                     /locus_tag="VFU15_06095"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012022830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HEU4717380.1"
                     /translation="MRSMTGFGKAVCELPQKKVTVEIRSLNSRQLDLNVRIPSLYREK
                     EAEIRAAISKNVERGKVDFSIYVEASGESGSATINEELATAYHRELKKLSDAIGEKRT
                     DFLSLIIRMPDVLRNDRQELNEEEWEQVSAAVGQAVSAFNGFRDDEGKVLSGEFVQRI
                     GIIRKKLEEIATLDPQRAANVRERLNKILSETTSAENIDKNRFEQELIYYLEKFDITE
                     EKLRLSTHCDYFLETMKEPSSGRKLGFISQEIGREINTIGSKANDAGIQKLVVQMKDE
                     LEKIKEQLLNVL"
     gene            complement(7846..8784)
                     /locus_tag="VFU15_06100"
     CDS             complement(7846..8784)
                     /locus_tag="VFU15_06100"
                     /inference="COORDINATES: protein motif:HMM:NF017807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEU4717381.1"
                     /translation="MNKTAPAHISLFISMAIYAAGFTIIKEVTPQHLGPMGFVALRLL
                     GATPVLWLAGLFLREKAERADIPKFALLSLCGVTINQTLFVRGMSLTSPINGAIIMIT
                     SPLLVLVLGSIILKERITLLRFAGIAVGLAGAVLLILTGTLANAREDSPLGDLFIFIN
                     ALSWGTFLVLVKPMMKKYSTVMILKWVFLFGMIIIVPLGIPDLQKADWKTFSESTLPD
                     GSFFGTVWFNIAFVVFGVTLIAYQLNTYALKALSPSVVSAYIYLQPVMAAGIALYFGK
                     DSLTWEKVLSAALIFTGVMMASKAKNVMKNDAAEKP"
     gene            complement(8829..9179)
                     /locus_tag="VFU15_06105"
     CDS             complement(8829..9179)
                     /locus_tag="VFU15_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020080798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HEU4717382.1"
                     /translation="MKKFYYLGTCDTCARIIKETGVDKKGFVMQDIKTEKITPAQIDE
                     MKKLAGSYEALFSRVALKYKTLDPKPSSEKEYRKLILGEYTFLKRPVAIMGDRIFIGN
                     SKSNVSALREALGK"
     gene            9368..10525
                     /locus_tag="VFU15_06110"
     CDS             9368..10525
                     /locus_tag="VFU15_06110"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017258341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEU4717383.1"
                     /translation="MKDASKYGFGTKAIHAGQEPDPSTGAIMTPIYQTSTYVQESPGK
                     HKGYAYARGRNPTRVALEKCIAALEGGSYGLCFSSGMGAIDAIIKLLRPGDEVITGDD
                     LYGGSYRMFTKVFANYGIKFHFISMTDAANIRKYVNANTKLIWAETPTNPTMQIIDIA
                     ACAAIAKEHKLILAVDNTFASPCLQNPLALGADIVMHSVTKYLGGHSDVVMGALVMND
                     AKLHEQLAFIANSCGATPGPMDSFLVLRGIKTLHVRMDRHCFNGRKIAGFLKTHPRIE
                     KIYWPGFEDHPNHAIAKKQMKDFGGMISIVLKGADLGETFRIASSFHVFSLAESLGGV
                     ESLINHPATMTHASIPKEERERVGVVDNLLRLSVGIEDVEDLLADLEQALK"
     gene            10537..11256
                     /locus_tag="VFU15_06115"
     CDS             10537..11256
                     /locus_tag="VFU15_06115"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU4717384.1"
                     /translation="MNHYFITGTSRGIGKAIALALLEDPGHHVTGLSRSCSISHPNYT
                     HISIDLSDAEEVKRFRFPALKDPGKIALINNSGTLGGVKYAGEMDAQRVTEAIHVNLL
                     APVCLTNEFLAAYVSENVTQVIINISSGAGKNPIDGWSVYCTAKAGLDMFSRVVAEEQ
                     KLRGRKNVHVFSVAPGIVDTAMQDEIRKSEKKNFSRIGQFSDYKTSGQLAEPALISAK
                     YLTILELPKKFTETVFSVKDV"
     gene            11404..>11833
                     /locus_tag="VFU15_06120"
     CDS             11404..>11833
                     /locus_tag="VFU15_06120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717385.1"
                     /translation="MMAKVFAAHPDIKAQFDANERLAEKQDSIDFSHNYYRQSQQAMM
                     PTVYTVPVVFHIIHQYGSENISDAQVLDEMRILNEDYAKLNPDTTAIESSFVSIAASA
                     NIQFKLANIDPNGNCTNGIDRVYSYLTNAADDNSKLDPWPR"
BASE COUNT         2602 a         3373 c         3083 g         2775 t
ORIGIN      
        1 tcctccttcg acctgcgcat cgatccgcgc acggcgacga ccgtgatgat ggtggcgggc
       61 ggttatcccg gcgattacga aaaaggaaag atcatttcgg gacttgagaa cgtgaaggat
      121 gccatcgcgt tccacgccgg aacgagatca gcgaacggaa acgttgtgac gaacggcggc
      181 cgcgtgatcg catttacggc attcggaaat tccatcggcg ctgcgctgaa aaattcctac
      241 gcagccgctt ccgcggtgaa gttcgacggc tgctatttcc gccgcgacat cgggcaggac
      301 ctgctgaaga tcgctgaaag cgcagcgaaa tagggaaaac aaaaaagccc ccgcaacgtg
      361 cggaggcctt cttcattgca aatggaatgt tatttgagtc cgccttcgcg gagctgcttc
      421 tggttgaact tgttctgcca ggctatccac caggcgaagc tgatgaagcc gatcgtgatg
      481 aggatcttgt tgggtgagag gtggtcgtat gtccactgga gtccgtcgaa gtatttgtaa
      541 caaacgcgtc cgaacgcgta ccagaaacct tcctgattca taagacgaat gtttttttag
      601 acagccccaa aggtaatcat tccagaaatc tatggtgtat ttttacagaa aattctgctc
      661 ttgttcatcc gcaacgcaag gtcgcaccgg ccggtttcgc tcatctttat cccgctcatc
      721 gcagtcggac tatggcttcc cgggttcctt cacccggcgc ccccggcccc gatgatcttc
      781 atgccggctt tcggttccgt tgcgtcgtgg acgtccgcct ggcctgtgct gaatgttttt
      841 ctcggcctgg cctttacact gctgcaggcc ttcctgttaa attacattgc gcagcagcag
      901 cagcttttta cacgaaaaac gtggctgccc gcgctgatgt tcgtcacgtt cgcttcgtgc
      961 acaccgtccc tgctgacctt caacccggag ctggtggcgg gactttttct tctcggcgcg
     1021 ctgtacgttt tgctcagcac ataccgcatg gaccgcgccc acagccaggt gttcaacgcc
     1081 ggatttctca tcgggctggc cgggatcttt tacctgcctt cgctcgtgtt tttcattttt
     1141 gccatcatag cgatcatcgt gctgcggccg ttcgtgtggc gcgagtgggc gatccttttt
     1201 gccggcctcc tcgtgccctg gatctattcc ggcgtttatt ttttctggaa cgaccggatc
     1261 gtatcggtga cgatggaaaa tatttacaac ccggttgtgc agcgcgattt tttcttcaag
     1321 ctgccggtgg aatattatgc gctcgcattt atgaccggca tggtgctgct tacttcggcc
     1381 ggccggttcg tggccggtgc gggaacatcg accatgaaaa cgaaaaaagg cgtttcggtg
     1441 atggtctggt tcctggtttt ctccatcatc gccgtcatcc ctgcacagaa tttcgcggtg
     1501 cccgttttcc tgttcacggt ctacccgctg tccgtgttca cgtcgaatta ttttctcaat
     1561 gcgcgccgcc agtggctcgc cgatacgatc tatctctttt tcctcgccag cattgcggcc
     1621 acgtacttca taaaagtttc cggtcttttc gggagctaat cgtatctttg caactccaaa
     1681 aaacaagcac atgaaattcg gcgtcgtgat ttttccggga tccaattgtg accaggatat
     1741 gatctatgtc ctcggcaaaa taatgggcca ggaagtggtg gaactgtggc acaaggatca
     1801 cgacctgcag caggtggatg ccgttgttct tcccggcgga ttttcctacg gcgattacct
     1861 ccgttccggt gcgatcgcgc gtttttcccc gatcatgaaa gaagtgatcg cattcgcgga
     1921 aaaaggcgga attgttttcg gcgtgtgcaa tggtttccag atactctgcg aagcaggact
     1981 gcttccggga gcgcttctgc agaataacga acggaagttc atctgcaaaa acgttttcat
     2041 tcgcgcacag aacaacagca cgctgcttac acaggcggtt ccgcaggaca aagccctgaa
     2101 aattcccatt gcgcacggtg aaggaaggta tttcgcttcg ccggaagtgc tgaagcagct
     2161 caacgacaac ggccaggtac tgttccgtta ttgcgacgag aacgggaacg tgaacactgc
     2221 cgcaaatccc aacggggcgg aagagaatat cgccgggatc tgcaatgccg gtaaaaatgt
     2281 tttcggcatg atgccgcatc ccgaacgcgc agccgatccc gagctcggca acaccgacgg
     2341 aaaattcctt ttccagtcta tcgtcagcct ggtcaatgcc tgaatgaacg ggtaagaccg
     2401 ccggatcttt tttttcattg gggaaagtaa gtgtacttac ctccaatttc tgtcgcagga
     2461 taagtaggat tcgtcgaaaa aggttttgtt gccgtctctt tttgtatgaa cttttgttcc
     2521 ggtaaactca aaatccattc tcaccttttc gcatttcacc taaccggttt ttccccatga
     2581 aaaaaattac tcttttactg tctgccgcgt gcatcgccgt tttttcaatg gcgcagcagc
     2641 cgcagataaa tccgccttcc caaccgcaga tcggcacgcc gcagtgggct gcgcagcacg
     2701 cacaggatcc cattgaacgc ctggccggtc ctgtatattc tccgcaaagc gggaacgaca
     2761 cgctgaacaa tgagtacacc aattccgcct gcggcctgaa ttattcgctc ggaactgttc
     2821 gtctcggcca gcgtttttca ccggtcggac ttccgcaacc tgcgggaatc gccgtgaaca
     2881 ttccgccctg cgcgacggtg ctcaaagcgt acctctacac ggaagcgctc ggcgtcgctc
     2941 cttccattac agccgatctc accgatcctg cgaacacgac aacgagttac aacatgtcgc
     3001 tcatcggcaa ttcgatcgat gtatgctggg gaatgaacgg cacgcacgtc tggagatctg
     3061 acgtcaccgc cagcatcagc ggacccggaa tgtattccat cagcgggctt cccgtttcat
     3121 cgacagcttc ttcgagcacg gtggatgtgg agggcgccac gctgctcgtc atttacgccg
     3181 atccgtacgc atcctacacc ggctcaatcg tcatcgacga cggttgccat acggtggccg
     3241 gcggtacgct cacgcataca atgacaggtt tcagcgcctg cgcgaattcc acgaacgcat
     3301 cttccttcat gctcgtcggc gatatgcaga tgatgggcta cacgatctcg atgaacggga
     3361 acaacgttcc ccagccgcag tgggactggt ggaaccagat ctcggcttcc acttccgtca
     3421 ccagcggaca aaacacgtgc acgttccaga tgaatgacat gggcgattgt tacacgcttg
     3481 cggtggccgg cctgtattac cagacgccct gccagacatg caccccgatg gcgaccaata
     3541 ttttactttc gacttcatcc accccggaaa cctgcaacgg caacggaaca gcttctgtga
     3601 cggcaagcgg cggcaccggg aattattctt acacatggct gccgggcgga tcaacctctt
     3661 caacggcgac aggacttaca gccggaacgt atacggttta tgtgcaggac ggaacttcct
     3721 gcgccagcgc aacggtgaat gtgggctaca acggcatggt actttctgta acagcatctc
     3781 ccatctcatg ttcttcgcag ggttcggcta cggtgaacgt caccggcgga acaggcccgt
     3841 tctcgtatgc gtggtcgcct tcgggtggaa cttctgccac ggcatccaac ctcgcagccg
     3901 gaacttacac cgttgcagtg acggatcagt ccaacggatg cgtgatgacc gccacctgta
     3961 cgatcgtcaa taattccacg ctgaccgcat acgtgtatac cacgccggac agcacctgca
     4021 gcgggaacgg cggcagcgca actgcttacg gttacggcgg aacgcagcct tacacatacc
     4081 tctggcagcc gggcggacaa accacgcaga cgattaccgg tcttgcgcaa ggttcttata
     4141 cctgcacgat caccgacagc gcaggctgct cggccacggc ttctgattcc gtcgccgcgt
     4201 attccctctg ggtgtacacg ggcggttacg gaatgtacag ctgcggcgac agcacacagc
     4261 tttatgcaat gtgcagcgat ccgtccgcaa cattcagctg gtcgccgccg acaggattga
     4321 gcaacccgaa tgtgcaggat ccttacctga ccgtttccgg taacatcacg tacacggtta
     4381 cggcaacgtc gggctgcggc actgcaaccg atacgttcat ggtcgttgtc gattcgctga
     4441 atatgtacaa cgagcagatc tgtttcgtca cagttgacac ggcaacgaac aagaacaaag
     4501 tgatctggga aagatggaat tccccgatga cgggctatta caatatttac cgtgaaacgg
     4561 cggtcagcgg gcaatacgcg ctcgtgggaa cacaacctgt ttcacagttc acaacattca
     4621 ttgacatgtc atcgaacccg gtcgtcatgg cgaaccgtta cgtgctcacc acggtggacg
     4681 gctgcggaaa tgaatcggat accagctatc accaccgcac gatccacctg cagacaagcc
     4741 ccggattttc gggtgcggtg aacctgcagt ggacggctta tgaaggactc ccgatctcga
     4801 cgtacaacat tttccgcgga ccttccatga gccagcttac gctgcttacg caggtggccg
     4861 ggaacgtgtt cacgtatacc gatgttacag cgccggccgg gccgctttat tacatggtgg
     4921 aagcggttca cccgtacggc ggatgttcgc cttcgcgcct cagccagaat gccacggatt
     4981 acgtcggttc gcagtcgaac atcgatttct tcaaccctga aggaatggct tcgcccgatc
     5041 tgcagaattc actgatgctc tcaccgaacc ccggcgacgg aagcttccgc ctctcggttt
     5101 atctcggcca ggcgcaaaac gtgcagacga cgatctatga caacctcgga agagtggttt
     5161 acgcttcttc acaggatgcc gcagcaggca cgttcacatc cgatatcgac cttcgcggca
     5221 ccagcgcggg aatgtatttc gtggaagtga aaacggagaa cggaacagcc gttaaacagc
     5281 tggtgataca atgacattct gattgatcca ggaaaaagag ccccggaaat ttcccggggc
     5341 tttttcgttt acaggaaatt ctgcagccac tttttcgctt ctgtttcgtt cgcaaaaagt
     5401 tttgtcggaa cgcccggttt gttcacgcgg ataaagaaat tgatgatgag gcgctgcgcc
     5461 gtcgaattgg taatgatcgc cacggcaagg aaacggtttt tatccgtctg cctggcaaaa
     5521 tattcgcgcg cctccccggt aatgctgaaa ttattcctcc catccacgat cgagacaacc
     5581 tgccggtttc ccaccatttc ggtccctata cgaatgagct cttccgcatc ctccagcacg
     5641 atctccgcac cttcgctgat gcggtagatg taatacgttc cttccagcgt gatctcgccg
     5701 gtccggtgct gcactgtctt gggcagaagc gtgttcattt ttcgtaaaag tgcatttcct
     5761 tcaggtattc ccaaacctgc ggtggaaaaa aataacggac gtcttttttg ttgcggatcg
     5821 cattgcgcag gaacgtggag gatatctcca tcagcggcgc ttccgtcatg atcacggaac
     5881 cgtgcgtggc aaattcgccc ccgtcagagt cgggacgcgg ataaacataa atggagtgat
     5941 gctcgaggat gaactcgtag tttttccatt tgcgcaggga ggcgagattg tcggcgccca
     6001 tgatgaggca gaatttttta gccgggtatt tttcggccag gtgcgccagc gtcgtaactg
     6061 tgtaggaagg ctgcggcagg ctgaattcga tattgctcgc tttcattttc ggatgatcct
     6121 cgatcgccat gcgcaccatg cgcaggcggt gattatcctg cagcagtcct tttttttctt
     6181 tcagcgggtt gtgcggtgac accacgaacc agatctcgga aaggtccgtg aacgcgagca
     6241 tgtaattcgc cagcacgaga tgcccgacgt gaatgggatt aaatgagccg aagaaaagtc
     6301 cggttttcaa tgctgttttt tttcgatgaa tccgccgacg atcccgtccg cttccgcaaa
     6361 ggctttctgc aggtcctcgt tcacgataac gcgatcgaag gaaggctcga acgtcagctc
     6421 atgcacggct ttgcttacgc gcatgcggat cttttcttcc ggctccgtgg cccgctgccg
     6481 caatcttttt tcaagcgctt ccaccgaagg cggccgcacg aaaacggaaa gcgccgcacc
     6541 gccgaatttt tttttcaggt tcagcccgcc cgtcacgtcc acatcgaaga tcacggtgtg
     6601 gcccttgcgc cagatccgtt ccacttccga atgcagcgtg ccgtaatatt gtcccgggta
     6661 cacttcttcc cattccacga attccccggc gtccacgcgg cggcggaatt catccgctga
     6721 aagaaaataa taatccacac cgtgctgctc ttcgccgcgc ggtgcacggc tggtggcgga
     6781 aactgaaaat tcaagcgaag ggaaccggcc cagcaggtga tggacgatcg tggtctttcc
     6841 tgctcctgaa ggcgctgaaa aaataacgag tttccctttt tcctgcatgt ggcaaaatta
     6901 cgaaggacgc atggaaatag ctcccggaga atgcgtcgcg gtcagagtac gttcagcagc
     6961 tgttctttga tcttttccag ctcatctttc atctgcacga cgagcttctg gatccccgca
     7021 tcgttcgctt tggagccgat cgtgtttatt tctcttccta tttcctgtga aatgaaaccg
     7081 agcttccttc cgctcgacgg ttctttcatc gtttcgagga aataatcgca gtgcgtgctg
     7141 aggcgaagct tctcctcggt gatgtcgaat ttttcgaggt aataaatgag ctcctgctcg
     7201 aaacggttct tgtcgatatt ctcggcgctc gtggtttcgg aaaggatctt gttcaggcgc
     7261 tcgcgcacgt tggcggcgcg ctgcggatcg agcgtggcga tctcttccag ctttttgcgg
     7321 ataatgccga tacgctgaac gaactcgccc gacagcactt tcccttcgtc atcgcggaag
     7381 ccgttgaacg cgctcaccgc ctgtccgacc gctgcggaaa cctgttccca ttcttcttcg
     7441 ttcagctcct gccggtcgtt gcgcagcaca tccggcatgc ggataatgag cgagagaaaa
     7501 tccgtgcgtt tttcgccgat cgcatccgag agttttttca gctcgcggtg atacgccgtc
     7561 gccagctctt cgttgatcgt ggccgaacct gattcgcccg aggcttccac gtaaatggaa
     7621 aaatccactt tgccgcgttc gacgtttttc gagatcgccg cccgtatctc agcttctttt
     7681 tccctgtaaa gcgacgggat gcgcacgttc aggtcgagct gcctgctgtt cagtgaacgg
     7741 atctcgaccg tcactttttt ctgcggcaat tcacatacgg ccttgccgaa accggtcatt
     7801 gatctcatag gaaacgaaaa tacaaaagtt aaggattgcg gctcattacg gtttttccgc
     7861 ggcgtcgttc ttcatcacgt tcttcgcctt gctggccatc atcactccgg tgaagatcag
     7921 cgctgcggaa aggactttct cccacgtcag cgaatctttc ccgaaataca gcgcgatccc
     7981 cgccgccatc accggctgca gataaatgta ggcgctcacc accgacgggc tcagcgcctt
     8041 cagcgcatag gtgttcagct ggtacgcaat gagcgtcacg ccgaaaacca cgaacgcgat
     8101 attgaaccac accgttccga agaacgaacc gtccggcagc gtgctttctg aaaaagtttt
     8161 ccagtccgct ttctgcagat cgggaatgcc gagcggaacg atgatgatca tcccgaaaag
     8221 aaaaacccat ttcaggatca tgaccgtgga atatttcttc atcatcggct tcaccagcac
     8281 gaggaacgtt ccccacgata acgcgttgat gaagatgaac aggtcgccca gcggactgtc
     8341 ttcgcgtgcg ttcgcgagcg tgcctgtgag aatgagcagc acggcgccgg caagaccgac
     8401 ggcaattccc gcgaaacgca gcagcgtgat gcgctccttc aggatgatgc tgcccagcac
     8461 gaggacgaga agaggagacg tgatcatgat gatcgccccg ttgatcggcg aggtgaggct
     8521 catgccccgc acgaaaagcg tctggttgat cgttacgccg caaagcgaaa gcagcgcgaa
     8581 tttcgggatg tctgcacgtt ccgctttttc gcggaggaaa agtccggcca gccagagaac
     8641 gggcgtggca ccgagcaggc gcagcgccac gaatcccatc gggccgaggt gctgcggcgt
     8701 cacttccttg ataatggtga atcccgcggc gtaaatggcc atcgagatga aaagcgagat
     8761 gtgcgcaggg gcggttttgt tcacaaatga atggttggaa attccagtct tccggtccgg
     8821 gtgttcagct atttcccgag cgcctccctt aaagcggaaa cgttgctctt cgaattcccg
     8881 ataaaaatcc tgtcgcccat gatcgccacg ggacgtttca ggaatgtgta ttccccgaga
     8941 atgagcttcc tgtattcttt ttctgacgaa ggcttcgggt cgagcgtttt gtatttcagc
     9001 gccacgcggc tgaacagcgc ctcgtagctt cccgccagct tcttcatctc gtcgatctgc
     9061 gcaggcgtga tcttctccgt tttgatatcc tgcatcacga accctttttt atccacgccc
     9121 gtttctttga tgatgcgcgc gcaggtgtcg cacgtgccga ggtagtagaa ttttttcatg
     9181 cgtcaaaagt attgctttgc agcgaaccgg ttgcgctttt tattctcgtc cgttcgggct
     9241 ttcgggtcgc ggagcttccc tcaatcccgg ctatgtacag gacttttccg tgcgtcaaag
     9301 gttttttccg ttcccggtgt ttcggcggcc cctaattttc ccaattttac acttcatttt
     9361 tcctgttatg aaagacgcat ccaagtacgg cttcggtacg aaagccatcc acgccgggca
     9421 ggaacccgat ccttccaccg gcgccatcat gacacccatc taccagacgt caacgtacgt
     9481 gcaggaatcg ccggggaagc acaaaggcta cgcctatgca cgcggcagga atcccacgcg
     9541 cgtcgcgctt gaaaaatgca tcgccgcgct cgaaggtggt tcgtatggtc tctgcttcag
     9601 ctcgggaatg ggcgccatcg acgcgatcat caagctgctc cgtcccggcg atgaagtgat
     9661 caccggcgat gatctctacg gcggctcgta ccgcatgttc accaaagtgt tcgccaacta
     9721 cggaataaaa ttccatttca tcagcatgac cgatgcggcg aacatccgca aatacgtcaa
     9781 cgcgaatacg aagctcatct gggccgaaac acccacgaac cccacgatgc agatcatcga
     9841 catcgccgcc tgcgcggcca tcgcgaaaga gcacaagctc attcttgcgg tcgacaacac
     9901 gttcgcttcg ccctgcctcc agaacccgct ggcgctcggc gcggatatcg tcatgcattc
     9961 ggtgaccaaa tacctcggcg gccacagcga cgtcgttatg ggcgcgctcg tcatgaacga
    10021 tgcaaagctc catgagcagc tcgcgttcat cgcgaactcc tgcggcgcca cgcccggacc
    10081 catggattca tttctcgttc tccgcggaat aaaaacgctg cacgtgcgca tggaccgcca
    10141 ctgcttcaac ggcaggaaga tcgcgggatt cctgaaaacg catccgcgca ttgagaaaat
    10201 ttactggccc ggcttcgaag atcatccgaa tcatgccatc gcgaaaaaac agatgaagga
    10261 tttcggcggc atgatctcca tcgtgcttaa aggcgcggac ctcggagaaa cgttccgcat
    10321 tgcttcctcc ttccacgttt tttcactggc cgaatcgctg ggcggcgtcg agtcgctcat
    10381 caatcatccc gcaacgatga cgcacgcttc catcccgaaa gaagaacgtg agcgcgtggg
    10441 cgtggtggac aatcttctcc gcctcagcgt cggcatcgag gatgtcgagg acctgctcgc
    10501 cgacctggaa caggccctga aataattccg cgaacgttga accactattt tatcacaggc
    10561 acatcgcgcg ggatcgggaa agcgatcgcg cttgcgctgc ttgaagatcc tggccatcat
    10621 gtaacgggac tttcgagaag ctgcagcatt tcgcacccca attacacgca catcagcatc
    10681 gatctttccg atgcggaaga agtgaagcgg ttccgtttcc cggcgctgaa agatccgggg
    10741 aagattgcgc tgatcaataa ttccggcacg ctgggcggtg tgaagtatgc aggagaaatg
    10801 gatgcgcagc gggtcacgga agcgatccac gtcaacctgc tggcgccggt gtgccttacc
    10861 aatgaatttc tcgccgctta cgtttcggaa aacgtcacgc aggtgatcat caacatctct
    10921 tccggcgcgg gaaaaaatcc cattgacgga tggagcgttt attgtactgc caaagcaggg
    10981 ctcgatatgt tctcccgcgt cgtggcagaa gagcagaagc tgcgcgggcg gaaaaatgtt
    11041 cacgtgttct ccgtggcccc cggcattgtc gacaccgcca tgcaggacga gatccggaaa
    11101 tcggagaaga agaatttcag ccgcatcggg cagttcagcg actataaaac gagcggccag
    11161 cttgcagaac cggccttgat ttcggctaaa tatttgacta tcttagagct ccccaaaaaa
    11221 ttcacggaga ccgttttttc cgtgaaagac gtctaagtat caagcacact aatacaacat
    11281 ccaaatcatt cattcatgaa aaagaatacc cttttcaccg gactttttct ggctgcttca
    11341 atttttgcgg caaaagcaca gtccccttat agtggggaag acctgggctg cggtaccgat
    11401 atcatgatgg caaaggtctt cgccgctcat cccgacatca aggcgcagtt cgatgcgaac
    11461 gaacgtcttg ccgaaaagca ggattcgatc gatttcagcc acaactatta ccgccagtcc
    11521 cagcaggcga tgatgcccac cgtatacacg gtgcccgttg tttttcacat catccaccag
    11581 tacggctctg aaaatatttc ggacgcacag gtgctggatg aaatgcgcat ccttaacgag
    11641 gattatgcaa agctgaaccc cgacactacg gcaattgaat cttcctttgt cagcattgcc
    11701 gccagcgcga acatccagtt caagctcgcc aatatcgatc caaacggcaa ctgcaccaat
    11761 ggcatcgacc gcgtgtacag ctatctcaca aacgcggcgg atgataattc caagctcgat
    11821 ccctggccga ggg
//
LOCUS       DASTSF010000209         4375 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1435086,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000209 DASTSF010000000
VERSION     DASTSF010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4375
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1435086"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="VFU15_06125"
     CDS             complement(<1..180)
                     /locus_tag="VFU15_06125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717386.1"
                     /translation="MHPIGIVFYAALMDHWRQNGITRKASIDYRIRSIPYLQRMGLFS
                     ALNYKDPISTKTHDET"
     gene            complement(692..1369)
                     /locus_tag="VFU15_06130"
     CDS             complement(692..1369)
                     /locus_tag="VFU15_06130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717387.1"
                     /translation="MDPKRSLGVIFLLQFAFSLKAGNFESRYGIGVVHVEPYNFFSIQ
                     FWKKPSDVIPYEQVNFLRDSSGKFLHFYFSDADTDSAPAWFAPEIFTMKSDTPRIDMF
                     CIEQSGDWCNVIVNSGTGKSMWIHTGPDTKFRTWEEFYAEFSMITLTDAEAQIYPKPD
                     LKAKHKKVTAKLSPGQAQKILPGAIQDRWMNVTVIVTGPDGKEVSKTTGWILWRDDDK
                     PLVNYTR"
     gene            complement(1407..2534)
                     /gene="hemW"
                     /locus_tag="VFU15_06135"
     CDS             complement(1407..2534)
                     /gene="hemW"
                     /locus_tag="VFU15_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002672828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HEU4717388.1"
                     /translation="MSHLYIHIPFCRQACHYCDFHFSTTLHSRGEMVKAIAKEISLRK
                     DELDEKKLSTIYFGGGTPSLIGADELKRIFEAIAKHFSIDENAEITLEANPDDLSKEF
                     LAGLRTTPVNRLSIGIQSFREEDLRMMNRAHNAAQAESSLRMARDAGFGNITADLIYG
                     IPGMSDEEWRKNISHLLSFAVPHISAYCLTVEPKTALAHFVREGKAKPVDEEEAASHF
                     QVLAEETAAAGFMQYEISNFARPGFIARHNSSYWFGEEYLGVGPSAHSFRGKVRRKNV
                     SSNPLYLQSLAQNKTAFEEETLSSDARFNEFVMTRLRTMWGIPLEEVRTVFGETYYKN
                     LISASRKWISEGKMTGENGTLVLTHGGKLLADSIASDFFMV"
     gene            complement(2571..>4375)
                     /locus_tag="VFU15_06140"
     CDS             complement(2571..>4375)
                     /locus_tag="VFU15_06140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717389.1"
                     /translation="NSNFSKRKFYYTIYYQDDSYKFPEYSEAGGMKYENRLSSRNFYG
                     SWGETEESFRPTKELAWGEQTTVTDSFRIVGNPRNEPACFGRMDQSGISALKIANMIN
                     YIHTRADWMLDVQVKAGRNHCTIEQQVFRDAVWQIDDQRKKKEGNNRWKRNPRAGTYK
                     FMLVVTTYDALQDVPVSVRDLTKSEPNGHAINPFYFFLYGTGSVDKRMKVLVADEQLS
                     VKVKFETSSGVYADPFQLTETGIDTSSFCATCGTDSLLYRRAQFEQLNYFEDHSNVLN
                     TIPVTADVADSNYTRAQYEANKEKYADENSRVQDIVRNFRRPCETVKVDSATGAIELI
                     NPGNPAPPYRKENVGVRSRIGLTYGKYRVCVAFPKIISQDHVWNGICNAVWLLYQDDA
                     PWNARKDCAGGYIAKSDNRKKQAERQPTLNYSEIDMEMVKASGNWPASSYGKNPAPAD
                     DHPENNDDVLLACTNWDMACHEPKKYVEGVVPLFFRKRKFDLHRWDYWYQAVTIRTVF
                     DHNEIFNRPYYWYGIDWTPDHITWSVGPDKDHMTVIGYMDDSVTAIPDNQMSLVVTQQ
                     FHNTEWWVPAPFDQRYIPYPKHPIVGRVLAVEVE"
BASE COUNT          973 a         1156 c         1078 g         1168 t
ORIGIN      
        1 tgtttcatcg tgagtcttcg ttgaaatcgg atccttgtaa tttaatgcag aaaacaaccc
       61 cattctttgt agataaggaa ttgatctaat tctataatca atggaggcct ttcttgtaat
      121 tccgttttgt ctccaatggt ccattaatgc ggcatagaaa actattccga ttggatgcat
      181 ggcaacatac gaaggatgaa atgatatttg gacattcttg tcagagaagt tgaaatcatt
      241 gtttttaaag accgtatcaa aacttctcaa cgagatacta ttgggtatat ataaattact
      301 catattgatt tttctaatgg tagctaacta gctaatagct gtaacaaaag ttcgtctatc
      361 ctgtcaatat gcaatgttag ggaatgtttt gatgcgtatt atttctttga aatttactaa
      421 ataaactaca atgtatcaaa agacatacgg atcttttgca ggttctgcgc actaacatgt
      481 gtgtagattt gcgtcgtctt gctgcttgaa tgccgcaaca actcctggat atagtactaa
      541 cttggactca gcagcaaacc tctccgtggt tcaggcattt tcatgactga acattatgac
      601 ttgcagattt tgggtattac caaaaattcg acaaaaggaa acgtaagcct tttcaactct
      661 taattcctga tcattactct ctgattcgca atcaccgggt gtaattcacc aatggtttat
      721 cgtcgtcgcg ccagagtatc cagcccgtgg ttttactcac ttcttttccg tcaggaccgg
      781 tgacgatcac cgtcacgttc atccacctgt cctgtatcgc gccgggcaga attttctgcg
      841 cctgcccggg agataatttc gctgtcactt ttttgtgctt tgctttcagg tccggtttcg
      901 ggtagatctg cgcttccgca tcagtgagcg tgatcatgga aaattccgcg tagaattctt
      961 cccacgtccg gaatttcgta tccggcccgg tgtgtatcca catcgatttt cccgtcccgg
     1021 agtttacgat cacgttgcac cagtcgccac tctgctcaat gcagaacata tcgatgcgcg
     1081 gggtatcgct tttcatcgtg aatatttccg gggcgaacca ggcgggcgca ctgtccgtgt
     1141 cggcatccga aaaataaaag tggaggaatt tacccgagga gtcgcggaga aaattcacct
     1201 gctcgtaagg gataacgtcg gaaggttttt tccagaattg tatcgaaaag aaattatacg
     1261 gttccacatg caccacaccg atcccgtacc tgctttcaaa atttccggcc ttaagggaaa
     1321 aagcgaactg caaaagaaaa attacgccaa gagaacgttt cgggtccatg cccgcgtggg
     1381 tttgctcaaa ctaaatttag caaaaatcag accataaaaa aatcggaagc gatgctatcg
     1441 gcgagcagct ttcccccgtg tgtgagaacg agcgttccgt tttctcctgt cattttccct
     1501 tcggaaatcc acttgcggga agctgaaatt aaatttttgt aatatgtttc cccgaagacc
     1561 gtccgcacct cttccagcgg aattccccac atggtgcgaa gacgcgtcat cacgaattca
     1621 ttgaaacgcg catcgctgct cagcgtttct tcttcgaacg ccgttttgtt ctgcgcaagc
     1681 gattgcaggt acaacgggtt cgacgaaaca ttctttcgtc tcaccttccc gcggaagctg
     1741 tgcgcggaag gacccacgcc gaggtattct tcaccgaacc agtaactgct gttgtgccgg
     1801 gcgataaaac ccggccgcgc aaaatttgaa atttcatact gcatgaatcc tgccgccgct
     1861 gtttcttccg ccagcacctg gaaatgcgaa gccgcttcct cttcatcgac cggtttcgct
     1921 ttcccctccc gcacaaaatg agccagcgcc gttttcggtt ccaccgtcag gcagtacgca
     1981 gaaatatgcg ggacagcgaa cgaaagcagg tgcgaaatat ttttccgcca ttcttcatca
     2041 ctcatgccgg gaatgccgta aatgaggtcg gcggtgatgt tgccgaatcc cgcgtcccgc
     2101 gccatgcgca ggctgctttc cgcctgtgcg gcgttgtgcg cgcggttcat catgcgcagg
     2161 tcttcttcgc ggaacgactg gatcccgatg ctcagcctgt tcaccggcgt ggtgcgcagc
     2221 ccggccagga attccttcga tagatcatcc gggttcgctt cgagtgtgat ctccgcattt
     2281 tcgtcaatgg aaaaatgttt tgcgatcgct tcgaaaatac gcttcagttc atcggcaccg
     2341 atcagcgacg gtgttccccc gccgaaatag atcgtcgaaa gttttttttc atccagctcg
     2401 tccttccgca atgaaatttc ctttgcaata gccttcacca tttcaccgcg actgtgcagc
     2461 gtcgttgaaa aatgaaagtc gcagtaatgg cacgcctggc ggcagaaagg aatatgaatg
     2521 tagagatgcg acaaaggaaa tgacggatca aaaatgataa acgtcccgca tcattctact
     2581 tccacggcga gcacccttcc cacgatcggg tgtttcgggt atggaatgta tcgctggtcg
     2641 aaaggcgcgg gcacccacca ttcggtattg tggaactgct gcgtgacgac gaggctcatc
     2701 tggttgtccg ggatcgcagt gacggaatcg tccatgtacc cgataaccgt catgtgatct
     2761 ttgtcggggc ccacgctcca cgtgatgtga tcgggcgtcc agtcgatgcc gtaccagtaa
     2821 tacgggcggt tgaaaatttc attgtgatca aacaccgtgc ggatcgtcac ggcctggtac
     2881 cagtaatccc agcggtgcag gtcgaatttc cgtttccgga agaacagggg cacgacgcct
     2941 tcgacatatt ttttcggttc atggcaggcc atgtcccagt tcgtgcacgc cagcagcacg
     3001 tcatcgttgt tctcgggatg atcgtcagcg ggagcaggat tttttccgta cgaggaagcc
     3061 ggccagttgc ccgacgcttt caccatttcc atgtcgatct cggaataatt cagcgtgggc
     3121 tgccgttccg cctgcttttt ccggttgtcg cttttcgcga tgtaaccgcc tgcgcaatct
     3181 ttccgcgcat tccatggcgc atcgtcctgg tagagcagcc acacggcgtt gcagatgccg
     3241 ttccacacat gatcctgcga aataatcttc ggaaatgcga cgcacacgcg gtacttcccg
     3301 tacgtcagcc cgatacgcga acgcacgccg acattttcct tccggtaagg cggcgcagga
     3361 ttccccgggt tgatgagctc gatagcgcct gtggcggaat ccactttcac cgtttcgcag
     3421 ggccggcgga aattccgcac gatgtcctgc acgcgtgaat tttcatccgc gtatttttcc
     3481 ttgttcgctt cgtactgcgc acgcgtgtaa ttgctgtcgg cgacatcggc cgttacggga
     3541 attgtattca ggacgttgct gtggtcctcg aaataattca gctgctcgaa ctgcgcacgg
     3601 cggtacagga ggctgtccgt tccgcacgtg gcgcagaacg acgatgtatc gatgccggtt
     3661 tccgtcagct ggaacggatc ggcgtacacg cccgacgacg tttcgaattt cactttcacg
     3721 ctcagctgct catcggccac gagcactttc atgcgcttgt cgacgctgcc tgttccgtac
     3781 aggaaaaaat agaacgggtt gatcgcatga ccgttcggtt cgctcttggt gagatcgcgc
     3841 acggaaacgg gaacatcctg cagcgcatca tacgttgtca cgacgagcat gaacttgtac
     3901 gttcccgcgc gcgggttgcg tttccaccgg ttgttccctt cttttttctt ccgctggtca
     3961 tcgatctgcc acaccgcatc acggaaaacc tgctgttcga tcgtgcagtg attgcgcccg
     4021 gctttcacct ggacgtcgag catccagtcg gcgcgcgtgt ggatgtagtt gatcatgttc
     4081 gcgatcttca gcgcactgat gccggactga tccattcgtc cgaagcacgc gggttcattg
     4141 cgcggattgc ctacaatgcg gaatgaatcg gtgacggtgg tctgctcgcc ccaggccagc
     4201 tccttcgtgg gacggaaact ttcctccgtt tcaccccacg atccgtaaaa gttccgtgat
     4261 gacaaccggt tctcgtattt cattccgccg gcttcgctgt attccgggaa tttgtaggaa
     4321 tcgtcctggt aataaattgt gtagtagaat ttccgcttgc tgaaattgct gttct
//
LOCUS       DASTSF010000210         4114 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1824306,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000210 DASTSF010000000
VERSION     DASTSF010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4114
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1824306"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..828)
                     /locus_tag="VFU15_06145"
     CDS             complement(<1..828)
                     /locus_tag="VFU15_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012796912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HEU4717390.1"
                     /translation="MNAKEIRRTFLDFFRSKGHEIVPSAPMIIKNDPTLMFTNAGMNQ
                     FKEWFLGNSPAKWPRVADTQKCLRVSGKHNDLEEVGVDTYHHTMFEMLGNWSFGDYFK
                     KEAIEWSWELLTKVYHLPEDRLYATVFEGSSDDGLSFDQEAFDHWKKFLPESRILKGN
                     KKDNFWEMGDTGPCGPCTEIHFDSRSDEERAKEYGAKLVNNDHPLVIEIWNNVFMEFN
                     RKADGSLEKLPAQHVDTGMGFERLVRVIQQKQSNYDTDVFQPVIRKVEEISGLRYTPG
                     "
     gene            complement(912..1481)
                     /locus_tag="VFU15_06150"
     CDS             complement(912..1481)
                     /locus_tag="VFU15_06150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717391.1"
                     /translation="MQATLRLLLSSALFIFLFSSLSAQDSVAYYPNGTKKFERHFNEK
                     GKKILTWYDGTGKKVRVSLFLTDDLVSDSLFDNGKLVSTGFRNKGEVYGHWTYYHTNG
                     TVALKGDYRFYRNLHIMGDTTAHLQSLTVKTGVWRSYDDKGRITRKEIYRHGYLRKYF
                     IYDTNGKSTTTERFRKCRSGNCPVKKAED"
     gene            1637..2608
                     /locus_tag="VFU15_06155"
     CDS             1637..2608
                     /locus_tag="VFU15_06155"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016419717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEU4717392.1"
                     /translation="MSKIRYRYNTKTLSYEKAVTPIGTRLFRILSFLGTSSVIGVISV
                     IIAFRYFDSPKERRLRREIDQMTLQYKLLNRRLDQYDLVLHDLASRDNDIYRAILETD
                     SIPDAIRRAGFGGADRYKDLEGFDNSALMISLTQKVDLIAKEMYIQSKSYDDVIKLTK
                     GKEKMLASIPAIMPIANKDLRHAPGGFGWRIHPIYKTPEFHPGMDFAAAEGTEIYATG
                     DGVVTVADDKSQGYGWHVVINHGFGYETLYGHMSRMAVVPGQQVKRGQVIGYVGSTGL
                     STAPHCHYEVHKNGELMNPVNYYYDDLTPEQYQLLIKMAAESGQAFD"
     gene            2668..3012
                     /locus_tag="VFU15_06160"
     CDS             2668..3012
                     /locus_tag="VFU15_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014680367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEU4717393.1"
                     /translation="MPYKEKETEKLYYSIGEVSKMFSVNASLIRFWEKEFEIIRPKKN
                     RKGDRMFTKTDIDNFRLIYHLVKERGFTLQGAKEKLKTNKDDAFNNVEMVKSLEAVKG
                     FLLELKKEISKE"
     gene            complement(3123..>4114)
                     /locus_tag="VFU15_06165"
     CDS             complement(3123..>4114)
                     /locus_tag="VFU15_06165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717394.1"
                     /translation="KLLKRVFLHAIEGESILFTDDGNIFATGFVTRYRPENYRGDLTQ
                     GVCAFLLDTALNVTWRETYPCFYNSVPHAAIQLRDHSFLVIASTLVYKDTVNWGADDR
                     DDNRMLIIGPGGKSVRDTILLSVKRDRYEQEYYDMYPHAKPEMPFCYLYNVVETDSGF
                     IFSGTFLASLSDWSVILIRTDKNFGLKKIRKISYKLSKYRKIPLWSQSMTCDTAGIVV
                     TARGEYSEDNNFIFRYSYDFDSLWMRQTPGKSLDPPVVSCTSAGHYLMCQSSGNRAYI
                     TFFDPSGNQETLPPGTKDIMEIDGICPDGNYAYIISSTKETPRQTYVMKIKLN"
BASE COUNT          946 a         1056 c         1117 g          995 t
ORIGIN      
        1 tcccggggtg taacgcagcc cggaaatctc ttccaccttg cggatcaccg gctgaaaaac
       61 atccgtgtcg taattcgact gcttctgctg gatcacgcgc acaagacgtt cgaagcccat
      121 gcccgtgtcg acgtgctgcg cgggaagttt ctccagcgag ccgtccgcct tgcggttgaa
      181 ttccatgaac acgttgttcc agatctcgat cacgagggga tgatcgttgt tcacgagctt
      241 cgcgccgtat tcttttgcgc gttcttcgtc gctgcgcgaa tcgaaatgaa tttcggtgca
      301 cggaccgcac ggacccgtat cgcccatttc ccagaaatta tcttttttgt tgcctttgag
      361 gatgcggctt tccggcagga attttttcca gtgatcgaac gcttcctggt cgaagctcag
      421 tccgtcgtcg ctgcttcctt cgaaaaccgt cgcgtacagg cggtcttcgg gaagatggta
      481 cactttcgtc agcagctccc agctccattc gatcgcctct tttttgaaat aatcaccgaa
      541 gctccagttg ccgagcatct cgaacatcgt gtggtgatac gtgtcgacgc ccacttcctc
      601 gaggtcgttg tgtttcccgg aaacgcgcag gcatttctgt gtgtcggcca cgcgcggcca
      661 tttcgccgga gagttgccga ggaaccattc cttgaactgg ttcattcccg cgttggtgaa
      721 catgagcgtg ggatcgttct tgatgatcat cggtgcggac ggaacgatct cgtgtccttt
      781 cgaacggaaa aaatcaagga aagtgcggcg tatctctttg gcgttcatac gaagtaaaat
      841 gaggcagcaa aaatacgaac caaaacggta ctgtggaggc ggcgttcttc cccgttatcg
      901 tgccgcgtca ttcaatcttc cgctttcttc accgggcagt tcccgcttct gcatttgcgg
      961 aagcgttccg tggtggtgct tttcccgttg gtgtcgtaga tgaagtattt ccggagataa
     1021 ccgtggcggt aaatttcctt ccgcgtgatg cggcctttgt cgtcgtagct tctccacacg
     1081 ccggttttca cggtaagcga ttgcaggtgc gccgtcgtat cgcccatgat gtggaggttg
     1141 cggtaaaacc ggtaatcgcc tttcagggca accgtcccgt tcgtatggta atacgtccag
     1201 tgcccgtaca cttcgccctt gttccggaag ccggtgctga cgagtttccc gttatcgaaa
     1261 agcgaatcgg aaacaaggtc atcggtgagg aaaagcgaaa cgcggacttt cttccccgtt
     1321 ccatcgtacc acgtgagtat tttctttccc ttttcgttga aatgacgttc gaattttttc
     1381 gtaccgttcg gataatacgc taccgagtcc tgtgcggaaa gcgatgaaaa aagaaaaata
     1441 aaaagggcgg aagagagcag taggcgcagt gtcgcttgca ttgcgccaaa agtaggaatt
     1501 cgctgcggaa atggattgcc ggcggttttg cgtctcacag cgatgaaacg cattttcggc
     1561 ccgggcccgt gcgggaaata atttaccggc attggaaacg gataaaatgg taaattaacg
     1621 ttcccaaaaa cggagcatgt cgaagatccg ctaccggtac aataccaaaa cgttaagcta
     1681 cgagaaggcc gtgacgccga tcggcacgcg cctgttccgg atactttcgt ttctcggcac
     1741 atcatctgtt atcggggtta tttccgtcat catcgcgttc cggtatttcg attctccgaa
     1801 agagcgcagg ctgcgccgcg agatcgacca gatgacgctg cagtacaagc tgctcaaccg
     1861 ccggctcgac cagtacgacc tcgtgctgca tgacctcgcc agccgcgaca atgacatata
     1921 ccgcgccatt ctcgaaaccg attccattcc cgatgccatc cggcgggccg gcttcggggg
     1981 cgccgaccgc tacaaagacc tcgaagggtt cgacaattcc gcgctgatga tctcgctcac
     2041 gcagaaagtc gatctcatcg ccaaagagat gtacatccag tcgaaatcgt atgacgacgt
     2101 gatcaagctg acgaaaggaa aagagaaaat gctcgccagc attcccgcca tcatgcccat
     2161 tgccaacaaa gatctgcgtc atgcgcccgg cgggttcggc tggaggatcc atcccattta
     2221 caaaacgccc gaattccacc cgggaatgga tttcgctgcg gcggaaggaa cggagatcta
     2281 tgcgaccggc gacggcgtgg tcacggtggc ggatgataaa tcgcagggct acggctggca
     2341 cgtggtgatc aaccacggtt tcggctatga aacgctgtac ggccacatga gccgcatggc
     2401 cgttgttccc gggcagcagg tgaagcgcgg gcaggtgatc ggctacgtag gcagcacggg
     2461 actttccacc gcgccgcact gtcattacga agtgcacaag aacggcgagc tgatgaaccc
     2521 ggtaaattat tattacgacg atctcacgcc cgagcaatac cagctgctca tcaagatggc
     2581 ggcagaatcc gggcaggctt tcgactgatg aaagcctgca gttgcgggca gttcttcaaa
     2641 cctggaattt taaactttga acgcacgatg ccttacaagg aaaaagaaac cgaaaagctg
     2701 tattacagca tcggtgaagt ttcgaagatg ttcagcgtga atgcgtcgct catccgtttc
     2761 tgggaaaaag agttcgagat catccgtccg aaaaagaaca ggaaaggcga ccgcatgttc
     2821 acgaaaacgg acatcgataa tttccgtctc atttatcatc tcgtgaagga gcggggcttt
     2881 acgctgcagg gcgcgaaaga aaagctgaag acgaacaagg atgatgcgtt caataacgtg
     2941 gagatggtga agtcgctcga ggcggtgaag ggtttcctgc tggagctgaa gaaggagata
     3001 tcgaaagagt gaaatttccc ggttgatgtt ccggtgtttt ataaccgcgt gtgtttgcga
     3061 ggagctttaa cgcagtgcac gctgagaatt tcttccgcag ctttccgtaa aacaggaaaa
     3121 cctcaattca gttttatttt cattacgtac gtctggcggg gtgtctcttt cgtgctggaa
     3181 atgatgtaag cgtaatttcc gtcaggacag atgccgtcta tttccataat gtctttcgtg
     3241 ccgggtggca atgtttcctg gtttccggac ggatcgaaga atgtaatgta cgcacggttg
     3301 ccgctgctct ggcacatgag gtaatgtccc gccgaagtac aggaaacaac cggcggatcg
     3361 agagacttgc cgggagtttg ccgcatccac agggaatcaa aatcgtaact gtaccggaag
     3421 atgaaattgt tgtcctccga atattctccc cttgcggtaa ccacgatacc cgccgtatcg
     3481 caggtcatgc tctggctcca caatggaatt ttccggtact tgcttagttt gtaagagatc
     3541 ttcctgatct ttttcaggcc gaaatttttg tcagtgcgga taaggatcac cgaccagtcg
     3601 gataagctgg ccaggaatgt tcccgagaaa ataaacccgc tgtcggtttc cacgacgtta
     3661 tacaggtagc agaacggcat ttcgggtttg gcgtgcgggt acatgtcgta gtattcctgc
     3721 tcataccgat ctcttttcac cgaaagcaaa atcgtgtcgc gtacgctttt cccgccggga
     3781 ccgatgatga gcatgcggtt gtcgtccctg tcgtcggcgc cccagttgac cgtgtctttg
     3841 taaacgagcg tggaagcgat gacgaggaat gaatgatcgc gcagctggat cgcagcatga
     3901 ggcacggaat tgtaaaagca cgggtaggtt tcgcgccagg ttacgttcag cgcagtgtcg
     3961 agcaggaatg cgcacacgcc ctgcgtcagg tctcctctgt agttttccgg gcggtatcgt
     4021 gtcacgaaac ctgttgcgaa aatatttccg tcatccgtga acaggatgga ttcaccttct
     4081 atggcatgca ggaaaactct tttcagcaat tttc
//
LOCUS       DASTSF010000211         2790 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_696167,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000211 DASTSF010000000
VERSION     DASTSF010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2790
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_696167"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="VFU15_06170"
     CDS             complement(<1..234)
                     /locus_tag="VFU15_06170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717395.1"
                     /translation="MFRFLLFPFFILLSAAGHAQHFSHFLYVHEAAGLNKQIPMAGYS
                     MAKKHIAYNFAAGKASGTDNQSLDPQDIDNINAK"
     gene            393..1760
                     /locus_tag="VFU15_06175"
     CDS             393..1760
                     /locus_tag="VFU15_06175"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEU4717396.1"
                     /translation="MRRYFLPSVFAATIFSVVVFYANCTPDLSVPRPQPGSCDFSKMV
                     AVGGDFMSGYMDGALSARGQERSVPALLARQLMLAGGGSFLQEKISVSGGLGWNLKPW
                     ESWFVNASKLGFKTDCQGVNSLMPVWDSLARANAAAEINSPASGSAQDFSVPFATMAQ
                     LFSPSLGNYSVSQNTNPYYHRIASNAGTSTVIGDAVNVNATFFSAWFGMEDIYNYAQQ
                     GGAGVSIPSAATFSAYLDSALSRLTQNGAKGVIANIPDFRDFPYYTLVPWNGAALTQS
                     KADSLNDIYQQAGFMNINFASGNNGFVIADANAPNGFRQMHGNEYITLSVPVDSLKCD
                     YLGLLFSVMPDRYVLDSAEVAYLDMNIAAYNSVIAQKAAQYNLALVDMNSYFRSFVSG
                     IKWDGVNYNAEFVTGGFFSLDGYHPNEKGDALIANEFITAINNKFGATVPTLNCFDCN
                     GVLFP"
     gene            complement(2304..>2790)
                     /locus_tag="VFU15_06180"
     CDS             complement(2304..>2790)
                     /locus_tag="VFU15_06180"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEU4717397.1"
                     /translation="LLVVDGSHSVDIRLNNLGIEHVFETWEGQNHVPEVGTSSAALAY
                     YDSALVITRNFLVHYICGDSLQSGYTTNVAVNNLAPLPSLIGVFPNPATDAFTIQSDR
                     LAGTGYIMDLYSSTGALVKSVPLSSSGQTTVSTNGLPAGMYFMNIHNADVSYSRKILV
                     Q"
BASE COUNT          602 a          690 c          751 g          747 t
ORIGIN      
        1 tttcgcattg atattatcga tatcctgcgg atcgagggat tggttatcgg tgccggaagc
       61 tttaccggca gcaaaattat acgcgatgtg ttttttcgcc atcgaataac ccgccatcgg
      121 gatctgcttg ttcagtccgg ccgcttcatg aacgtacagg aaatgggaaa aatgctgtgc
      181 gtgaccggca gcggaaagca gaatgaaaaa cgggaaaaga agaaagcgaa acatctgtcc
      241 gggataaaaa tacaaacgca tatacggggc ttgcataagt ttagttcagg ggtatgtaag
      301 aaaatcggct aatatgtaaa gattgaaggt gattgtgaat gcagcataag gcgggaatta
      361 agtgaaagaa aaatttgtag cttcggtttg ttatgcgcag gtattttctt ccttccgttt
      421 ttgctgcgac gattttctcc gtggtggttt tttacgcgaa ctgcacgccg gatctttctg
      481 ttcccaggcc gcagcccggc agctgtgatt tttcgaaaat ggttgccgtc ggcggtgatt
      541 ttatgtcggg ctatatggat ggtgcattgt ctgcgcgcgg acaggagcgt tctgttcccg
      601 ctttgctggc gcgccagctg atgctcgccg ggggcggaag ttttttgcag gagaaaatct
      661 cggtcagcgg cggactcggc tggaacctga aaccctggga aagctggttc gtgaacgcat
      721 cgaagctggg attcaaaacg gattgccagg gcgtgaattc gctcatgccg gtgtgggatt
      781 cgctggcgag agcaaatgca gcggcggaga tcaattctcc tgcatccgga tccgcccagg
      841 atttttccgt tccgttcgca acgatggcgc agctgttctc tccttcgctg ggaaattatt
      901 ccgtttcgca gaacacgaac ccgtattatc atcgcatcgc atccaacgcc ggaacatcca
      961 cggtgatcgg cgatgcggtg aacgtgaatg ccacgttctt ttccgcgtgg ttcggcatgg
     1021 aagacattta caattacgcg cagcagggtg gagcgggcgt cagcattcct tctgcggcga
     1081 cattttcagc ctatctcgat tcagcgctga gccgactcac gcagaacggt gcgaaaggtg
     1141 tcatcgcgaa tattcccgat ttccgcgatt tcccgtatta cacgcttgtt ccctggaacg
     1201 gcgcagcgtt aacgcaaagc aaggcggatt cgctgaatga tatttaccag caggccgggt
     1261 tcatgaatat caatttcgcc agtgggaaca acgggttcgt tatcgccgac gccaatgcgc
     1321 cgaacggctt ccggcaaatg cacggcaacg aatacattac actttctgtt cccgttgatt
     1381 cactgaaatg tgattatctc gggctcctct tttccgtcat gcccgaccgt tatgtgctcg
     1441 acagcgcgga agtggcgtac ctcgacatga atattgcggc gtacaattcc gtcattgcgc
     1501 agaaagcggc gcagtacaac cttgcgctgg ttgacatgaa ttcgtatttc cgctcctttg
     1561 tttcaggcat taagtgggac ggcgtgaatt acaatgccga attcgtcacg ggcgggttct
     1621 tttccctcga tggctatcac cccaatgaaa aaggcgatgc gctcatcgcg aacgaattca
     1681 taaccgcgat taataataag ttcggggcca ctgttcccac gctgaattgc ttcgactgca
     1741 acggggtgct gttcccgtaa atgtgcgtca ttgtgtacgc gggatttctg cttcaggaat
     1801 atttcagggt acaaccgcct gttctcgact atcgctcgaa ctgacggttg ttttcatttt
     1861 tgtccgtccc ggctatcgcc cgaacggacg gttgttttct tttttgtccg tcccgactat
     1921 cgcccgaaca gacagttgtt ttcttttttg tccgtcccgg ctatcgctcg aactgacggt
     1981 tgttttcttt tttgtccgtc ccggctatcg ctcgaacaga cagttgtttt cttttttgtc
     2041 cgtcccggct atcgcccgaa ctgacggttg ttttcatttt tgtccgtccc ggctatcgcc
     2101 cgaacagaca gttgttttct ttttgttcgc cccgactatc gcccgaacag acagttgttt
     2161 tcttttttgt ccgtcccggc tatcgctcga actgacggtt gttttctttt gtccgtcccg
     2221 tccagcgccc gggcgaataa tgagaaatga gctcatttgt gagatgagtt atggatcttg
     2281 tgccgctaac agagccggct ttattattgc accagtatct tccgcgaata ggacacgtcc
     2341 gcattgtgga tgttcatgaa atacatgccc gcaggaagac cgttcgtgga aacggtggtt
     2401 tgcccgctgg aagaaagcgg gacacttttc accagcgcgc cggtagaaga atacaggtcc
     2461 attatgtaac cggttcctgc gagacgatct gactggatgg tgaatgcgtc ggtcgcggga
     2521 ttcgggaaaa caccgatgag cgaaggaagc ggggcgagat tgttcacggc cacattcgtg
     2581 gtgtaaccgc tttgcagcga atcaccgcag atgtaatgca cgaggaaatt ccgtgtgatc
     2641 acgagtgcag aatcataata ggcgagcgca gcgctgcttg ttccgacctc aggtacatga
     2701 ttttgccctt cccatgtttc aaagacgtgt tcgatcccca ggttgttcag gcggatgtcg
     2761 acggaatgtg agccatcgac cacaagcagc
//
LOCUS       DASTSF010000212        12824 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_931675,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000212 DASTSF010000000
VERSION     DASTSF010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 12824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12824)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12824
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_931675"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1208
                     /locus_tag="VFU15_06185"
     CDS             <1..1208
                     /locus_tag="VFU15_06185"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HEU4717398.1"
                     /translation="YSSVEYKSTHTGKWDYAKSLALPPKLPLGVIGMTSRTDDANASF
                     TITFYKPVPETYNRLKIFCKKEHNAYDLLVKTGTKNIAVAVDSSSADTLPYYEVSLPP
                     LSDHITIQLQKNNPYENELEFYGMSLETSENRGLIFHNCGVGGAKYESVLYEDLFEKQ
                     LPALNPDLVIIDFGTNDYIYEDSIHPDLDSQIVAVIHRVRNAAPQASIILTSTQDMYR
                     KGHNMVCGEQFADLIRGIARQEHCGLFDWYWISGGRTARLKWNEKGYSQPDMIHLSPR
                     GYRLKANLFADAIVSTYHFLELNTTDDSLVFNMDSLKEVQKKLIVVDSLWNSTDTRGK
                     HMVRHTVRKGESLSRIASHYGVSIVQIKKWNHLNGDIIHVGQVLVIYTNQKPKPKPKP
                     KTKPAPPKK"
     gene            complement(1218..1772)
                     /locus_tag="VFU15_06190"
     CDS             complement(1218..1772)
                     /locus_tag="VFU15_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017729779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WcaF family extracellular polysaccharide
                     biosynthesis acetyltransferase"
                     /protein_id="HEU4717399.1"
                     /translation="MPENTTDLGKFNNDWYQPGASVLKRFLWHCFSACFFISRFPFNG
                     IKKGMLRMFGAKIGKGVVIKPSVNIKSPWLLEVGDHSWIGENSWIDNLVRVRIGANCC
                     ISQGALLLTGNHDYKKSTFDLVTGEIVLEDGVWIGAKSVVCPGVTCRSHSVLTVGSVA
                     TKEMEAYFIYRGNPAVKTRERKIS"
     gene            1866..4133
                     /locus_tag="VFU15_06195"
     CDS             1866..4133
                     /locus_tag="VFU15_06195"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU4717400.1"
                     /translation="MAPGKTPRPFFTRLFLVLALLLAGINAPAQKNKADSLAPLLKKN
                     PGDRVIRKNFISALRKMDADSALVFGVRAANDAEQKDDKIAAGDWNLYNASTLDVLNR
                     KKEAETSATKAYGLLKAAGDTMNLDDCANTLGYIKVRAGNFKEAKTYLEEALSLREAS
                     ADTAGLAGTLMNLGLLYGAMGDYNSSLDYYFKAVEYARPIEKKDPVYMGKILNNIGSI
                     YMNQKKYDDALQFLRQALSYKIKAGNKSDMASTYNNIGSCFMEEHVNDSALYYFGLAL
                     DIRKEAKDVRGEAIVHNNFGQVYENQGQFKKAEAEFRTALSLRETLPNQDDIASSLVS
                     LSEVLLKMNRAAEALPLARRSYELALQNRSLTQMLESNLCLFKVLVANGNYQEASKYA
                     QQGLDLKDSIQNDVTDKHAQEMKARYDMEGQTREIEKLKSKKREDDLRNATERKMLLT
                     ILLSGGVLVLLTGFFFWFRHRENQRSRKVLQMAYDQIETKNRNITDSITYARQIQLAI
                     LPEEKQLRQLFPGSFVFYKPKDIVSGDFYWVSATEGKRWIAVADCTGHGVPGAFMSVL
                     GSTHLSAAVAENPAGPLSLILDNLDKEIRRQLHQDSGISRDGMDIALCCYEPSTGVLA
                     FAGANRPMIVLRRDGSMEIFRPVKTSIGGMIAGTEKKFTETQIRLLPGDTFYLFSDGF
                     PDQFGSPEGSSGNHSLNGMLKGKKFKSSRFRSLITSVRSLEPEEQKKEIERTFLSWKG
                     DLEQVDDVCVIGVRV"
     gene            4255..5583
                     /locus_tag="VFU15_06200"
     CDS             4255..5583
                     /locus_tag="VFU15_06200"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEU4717401.1"
                     /translation="MGTYLVLIILLVLVNAFFVAAEYSIVKVRSSQVDLKVSKGSARA
                     KIVKHIIGHLDAYLSATQLGITFSSLVLGRIGEPYIADLLLKLFNKMHVTMSDGALHN
                     VSFVAGIGIITIVHMVIGEQVPKTVAIRHSLATSLLVAWPLRIYNLVFAPFVWIVKVL
                     TAFTLRLFGIKKTSEHDDVHTEEELRLILTESEEGGAIKPSEHDLIQNVFEFDDRAVR
                     SILVPAVKISAVDIDLAPHEILDKVVEEGYSRMPVYKGTIGNITGIIYAKDLLKLMKE
                     HKLTREAVEGMVRPAHFIPQNKRINELLREFQTLHIQMAIVTNEFGGIAGLVTMEDVI
                     EELVGEIQDEYDEEKPVVEKKSETEFLVSAMASIPDVNDHIPIALPESPHYETVSGLM
                     NYLFGRIPAVNEKKIFGGYEFLITKRLRHSVEQVKMTVTDSGQFNAEEKS"
     gene            complement(5599..6753)
                     /locus_tag="VFU15_06205"
     CDS             complement(5599..6753)
                     /locus_tag="VFU15_06205"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEU4717402.1"
                     /translation="MESKAEKRKVLVFIDWFLPGDKAGGPVRSVANLLDHFGSEFDFS
                     VVTRDTDYTSSVPYAGVKSDAWNKLPGGQRVFYVSGNKLSPGTIASILEKETYDVVYL
                     NGMWSKHFTIEPLQVLKRMKKNVRVVVAVRGMLAPSALAIKPLKKKLFLAYAKLRNLF
                     SGVIFHATTEEEAQQARKFFGEKNEIIVAGNLPRRSMSAGAGREKKKGAVRIVNVARI
                     APEKNTLYAVELLSMVKSRAEADFFGPVYNEEYAGQCREAAARLPENVKLDFRGALDS
                     GKIEETLPAYDLLLLPTRGENFGHIILEAMQCGVPVLISDKTPWKDLEKEKAGWVISL
                     DEKEKFAAVIDRVSEMDETEYREWSEGAKRAAEKYISSSSLPENNRKLFA"
     gene            complement(6737..8662)
                     /gene="asnB"
                     /locus_tag="VFU15_06210"
     CDS             complement(6737..8662)
                     /gene="asnB"
                     /locus_tag="VFU15_06210"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014797437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HEU4717403.1"
                     /translation="MCGINGIFDCNGGKENYREHVKRMNASLAHRGPDDEGIFSEEGI
                     TLGHRRLSIIDLSPGGHQPMQYEHAGKKYTLIFNGELYNFRELKTGLSGEGFRFRTHS
                     DTEVVLAAYAKWGTKCLDSFNGMFAFAIWSHSERTLFIARDRLGIKPLYFTIKDDRTI
                     IFSSEVRALLASGLTERKIYGRGLDDFLHYQTVHCPASIVANVTMLKPGCFLLAGKED
                     TFYEEGEKRGPAIQSASWWPALVPREKPVTYAEACTTVRKLLREAVECRLVSDVPFGA
                     FLSGGIDSGAIVALMSEVATAKVKTFSVTFDDSEFSEAVYARMIAKKFGTEHHEIKLS
                     PDDFLHELPAALDAMDHPSGDGPNTFVVSKATKEAGITMALSGLGGDELFGGYEIFKR
                     MVALEKRKWLWSLPLFSRKITGNFRKMMKPGVSSDKIAALMEMSSYDFYNAFMISRQV
                     LSGGQIALITNGWKTGSPAPGKMAADATRSWHFDSPGQVLSRISVAEIGTYMQNVLLR
                     DTDQMSMAHALEVRVPFLDYRLVEYVLSLPDEIKYPHTPKKLLVDSLGDLLPPEIVNR
                     PKMGFTFPWKKWMKNELKSFCAERLEDLGKREPFDPEGISRLWNSFLRDDPRVTWSRI
                     WPLVVLSHWLTKNGIES"
     gene            complement(8688..9737)
                     /locus_tag="VFU15_06215"
     CDS             complement(8688..9737)
                     /locus_tag="VFU15_06215"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEU4717404.1"
                     /translation="MKLLVLYEELAGYFLACINAFAERHAADVIIVRKKVNAVAPFVF
                     HESPRVKIVDRESFSEKELFDFAIGAAPGAIFCAGWGCKPYMTISRHFHGKIPVVLGF
                     DNWWDGSLKQLILAQGARVSFRRRFSACFVPGKPQELFAEKLGFPRDKIATGAYCCDY
                     DFFHGIFEKHEAAKKAHFPKRFIYAGRYAKEKNVEMLWEVFAELKKEIPGEWELWCLG
                     KGDIVPPEIEGVRHLGFVQTSGMEEVMSRAGVFILPSTFEPWGVVVHEYAAAGFPMIL
                     SDKVGAAEFFLEPGKNGWKFNSGNRDELKSAMREAMRSPVLRLAEMGGESARLASLHT
                     PALWADRLNALIAST"
     gene            9832..10800
                     /locus_tag="VFU15_06220"
     CDS             9832..10800
                     /locus_tag="VFU15_06220"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU4717405.1"
                     /translation="MEIEAGSYDKSMKQQVAALFSMQYGVSEKEFTDLIGNFYEHPYQ
                     AEKCIRIVALDGEKVVGFQSFFLWPYEYQGKVFRSYQSGNSLVHPDYRGKGIFQKLLH
                     YLEKKREELGVEFIMGFPINVSRNSLIRNGWKNIVDLQWYVKPVRFLPVFGGNEDALL
                     RRCFSAEAPALPSQPAQEHIRLTTDPFFREWRKNYSTATGHYYWFAYEEKDRKAVFQL
                     KLIRRKKLIRELIVGDVIAQDNSRAFLAAAFTALAKAARKTRCVHLISFAVNPASPYI
                     SHATLADAGMNTIDRHIFFAVKPFAEIPALSDPSKWILFRSDIDTW"
     gene            10801..11667
                     /locus_tag="VFU15_06225"
     CDS             10801..11667
                     /locus_tag="VFU15_06225"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEU4717406.1"
                     /translation="MKYDPKIHSWRHLVKSALGRMEFLLTGEPCPPGELLVLNYHGTP
                     LKFMDNFREQIRFFRDRFDFISPAQLGDYFSGKPDPKKSYALITFDDGLRNNLHAAEV
                     LREFNIRAYFFIVPAFINAPAGDQKNYYLQHIRPQINPAIDSREEDLLPLSWEDVRRL
                     HSEGHGIGAHSLTHDLLASGSEEESRKEIISCREVIYEKTGVMPDSFCSPNNSLLSAG
                     KKEMQLIRENYRYHFSTLPGSNTGASPFFIRRVNVESFWLKGAVMRATGKWDRKRWTS
                     RIMQFSAVVNGN"
     gene            complement(11668..>12824)
                     /locus_tag="VFU15_06230"
     CDS             complement(11668..>12824)
                     /locus_tag="VFU15_06230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717407.1"
                     /translation="FMLLFLIAVGLFGVLSDNNTLFLFIKIFIGLVLSASFYYYVFRH
                     YDFDVDFLFRMYLKGAYYAAVIGVVQFVAFRLHITPLYNFRWLFNKWGLPPGSVLGIR
                     INSVFSEPAQCAIVLSPAAMVGFYSIFFRDTSYYTVLRSLIVIGVFILTFSSTGYFGL
                     FIIILLLAVNFGQIANFVSALVFGAVAVFLLYRLVPEFQSRIDSTIELWIYQNYALEN
                     VNSSSFVLYNNFHVAWENFKSNFLFGTGLGSHQAAFDKYSLTKNEDILNFTFNQSDGN
                     SMFVRLMSETGLSGLVFMFVFLRRNFVRRTGELRNNTHWLISSAMMTIILLYLLRQGN
                     YFVNGFPFFMWMYYFNAVEYRKKMAMREEEAFDGEEEEVDGETEALPANA"
BASE COUNT         3208 a         3564 c         3268 g         2784 t
ORIGIN      
        1 cttactcttc cgttgaatac aaatcgacgc acacgggaaa atgggactat gcgaaaagtc
       61 ttgcacttcc gcccaagctc ccgctcggtg tgatcgggat gacttcgaga accgatgatg
      121 cgaatgcatc gttcaccatc acgttttaca aaccggttcc cgaaacctac aaccgcctga
      181 aaattttctg caagaaagag cacaacgcgt atgacctcct ggtaaaaaca ggaacgaaga
      241 acatcgctgt cgccgtcgat tcatcctctg cggatacgct tccgtattat gaagtttccc
      301 ttcccccgct gagcgatcac atcaccatcc agctgcagaa aaacaacccg tacgagaacg
      361 agctcgaatt ttacggcatg agcctggaaa cgtcagagaa ccgcggactt atttttcata
      421 attgcggggt gggcggcgcg aaatacgagt cggtgctgta tgaagatctt ttcgagaagc
      481 agcttcctgc gctgaatccc gatctcgtca tcatcgattt cggcacgaac gattacatct
      541 acgaagacag catccatccc gatctcgaca gccagatcgt cgcggtgatc caccgcgtgc
      601 gcaacgccgc gccgcaggct tccatcattc tcacgtcgac gcaggacatg taccgcaaag
      661 gccacaacat ggtgtgcggg gaacagttcg ccgatctcat ccgaggcatt gcacgacagg
      721 aacattgcgg cctgttcgac tggtactgga tctcgggcgg aagaacggcg agactgaaat
      781 ggaatgaaaa aggctattcg cagccggaca tgatacatct ttcaccgcgt ggctaccggc
      841 tgaaagcaaa cctgttcgcc gatgcgatcg tgagcacgta tcactttctc gaactgaaca
      901 ccacggacga ttcgctcgtg ttcaacatgg acagcctgaa agaagtgcag aaaaagctga
      961 tcgtcgtgga ttcgctctgg aacagcacgg atacacgcgg caagcacatg gtaagacaca
     1021 ccgtgcgtaa aggagaatcg ctgagccgca tcgccagtca ttacggcgta tcgatcgtgc
     1081 agatcaaaaa atggaaccac ctcaacggcg atattatcca cgtcgggcaa gtgctcgtca
     1141 tctatacgaa ccagaaaccg aaaccgaagc cgaaacccaa aaccaaaccg gctccgccaa
     1201 aaaaatagag taagcggtca gctgatcttc cgttcccgcg tttttaccgc ggggttcccc
     1261 cggtagataa aatacgcttc catctctttc gtcgcgaccg acccaacggt aagaacggaa
     1321 tgtgacctgc aggtcacgcc ggggcacacc accgacttcg caccgatcca cacgccgtct
     1381 tcaagcacga tctcaccggt tacgaggtcg aacgtgctct ttttgtaatc gtgattcccg
     1441 gtcagcagca acgcgccctg ggaaatgcag cagtttgcac cgatgcgcac gcgcaccagg
     1501 ttatcgatcc aggaattttc accgatccag gaatgatcgc cgacctcgag cagccaggga
     1561 cttttgatgt tcacggaagg cttgatcacc acgcccttcc cgatcttcgc accgaacatg
     1621 cgcagcattc ctttttttat tccgttgaac ggaaaacggg aaatgaaaaa acaggcgctg
     1681 aagcaatgcc agagaaaacg tttaagcacg gaagctcccg gctggtacca gtcgttattg
     1741 aatttaccaa ggtccgtcgt gttttccggc atgcgcccaa aggtaagaaa ttgcgatgcg
     1801 acggcttccg ggctctgcag cgggccttta cggataaata ttctatctta atgcctctga
     1861 aaaatatggc tccgggcaaa acacctcgtc ccttttttac acgcctattc ctggttcttg
     1921 cattgcttct cgcaggaata aatgcgcctg cgcagaaaaa caaagccgac agcctggctc
     1981 ccctgctgaa aaaaaatccc ggcgaccgcg tcatccggaa aaattttatt tctgcgctcc
     2041 gcaaaatgga tgcagacagc gcgcttgttt tcggcgtccg cgcggcgaac gatgcagaac
     2101 agaaagacga caagatcgct gccggcgact ggaacctgta caatgcatcg acgcttgacg
     2161 tgcttaaccg gaaaaaggaa gcagagacca gcgcgaccaa agcttacggc ctgctgaaag
     2221 ccgcgggaga cacaatgaac ctggacgact gcgcgaacac gctcggctat atcaaggtgc
     2281 gcgccgggaa tttcaaagaa gccaaaacct atctcgaaga agcgctttcg ctgcgcgaag
     2341 cgtcggcaga tacggcggga ctggccggaa cactcatgaa cctcgggctg ctttacggcg
     2401 caatgggcga ttacaacagc tctctcgatt actatttcaa ggcggtcgaa tatgcgcggc
     2461 cgatcgagaa aaaagatccc gtttacatgg ggaagatcct caacaacatc ggttccattt
     2521 acatgaacca gaagaaatac gacgatgcgc tgcagttcct ccgccaggcc ctgtcgtaca
     2581 agatcaaagc cgggaacaag agcgacatgg cctccacgta caacaacatc gggtcgtgct
     2641 tcatggaaga gcatgtcaac gattcggcgc tgtattattt cggtcttgcc ctcgacatcc
     2701 ggaaggaagc gaaagacgtg cgcggcgaag ccattgtgca caacaacttc ggccaggtgt
     2761 acgaaaacca ggggcaattc aaaaaagcgg aagcggaatt ccgcacggcg ctttccctgc
     2821 gtgagacgtt gcccaaccag gatgacatcg ccagctcgct cgtttcgctt tccgaagtgc
     2881 tgctgaaaat gaaccgtgcc gcagaagcgc ttccgcttgc ccggaggtcg tacgaactcg
     2941 cgctgcagaa ccggagcctc acacagatgc tcgaatcgaa tctctgtctt ttcaaagtgc
     3001 tggtcgcgaa cgggaattac caggaagctt caaaatatgc ccagcaggga ctcgacctga
     3061 aagattcgat acagaacgac gttaccgata agcacgccca ggaaatgaaa gcgaggtacg
     3121 acatggaggg ccagactcgc gagatcgaaa agctgaaaag caaaaaacgt gaggacgatc
     3181 tccggaatgc aaccgaacgc aaaatgctgc ttaccattct tctttccggg ggagtgctcg
     3241 tgctgctcac tgggttcttt ttctggttcc gtcacaggga aaaccagcgt tcacggaaag
     3301 tgctgcaaat ggcgtatgac cagatcgaaa ccaagaacag gaacatcacc gacagcatta
     3361 cgtatgcgcg gcagatacag ctcgccatcc tgcccgaaga aaagcagctc cgccagctgt
     3421 tcccgggcag cttcgttttt tataaaccga aagacattgt ttcgggcgac ttttactggg
     3481 tcagtgcaac ggaaggaaaa cgctggatcg ccgtggcaga ctgcacaggg cacggcgttc
     3541 ccggcgcatt catgagcgtg ctgggcagca cacatctttc cgcagcagtc gctgaaaacc
     3601 cggcaggacc tctttcgctt atactggaca atctcgacaa agagatccgc cggcagctgc
     3661 accaggacag cggcatttcc cgcgacggga tggatatcgc gctctgctgt tacgaacctt
     3721 ccacaggcgt gctggcgttt gccggcgcca accgcccgat gatcgtgctg cgcagggacg
     3781 gttcgatgga aattttccgc ccggtgaaaa catcgatcgg cgggatgatc gccggaacgg
     3841 aaaagaagtt taccgaaaca cagatccgtc ttcttcccgg cgacacgttc tatctttttt
     3901 cggacggttt ccccgaccag ttcggttcac ccgaaggaag cagcgggaac cattccctca
     3961 acggcatgct caaaggaaaa aaattcaaat cgagccgctt ccgttcactc atcacttcgg
     4021 tccgttcgct cgaacccgaa gagcagaaaa aagagatcga aagaacattc ctgtcctgga
     4081 aaggcgatct cgagcaggtg gatgatgtgt gcgtgatcgg tgtacgggta tgaaatgtca
     4141 ggcttgagac ctgaaaccag gaacttttaa acccggatcg cgtgcgcgga acggatgaag
     4201 caagacctcc ctttttaata tctttgtcca tatcaacgca ccacggttcc cgctatgggt
     4261 acatatcttg ttctgatcat tcttcttgtt ttagtgaatg ctttttttgt tgcagcggaa
     4321 tattcgatcg ttaaagtaag atcctcgcag gtcgatctta aagtcagcaa aggaagcgcg
     4381 agagcgaaaa tcgtcaagca cattatcggt catctcgacg cttatctttc cgcaacgcag
     4441 ctcggcatta cgttctccag ccttgtgctc ggccgtatcg gcgaaccgta cattgccgat
     4501 ctcctgctga aactcttcaa taaaatgcac gttacgatga gtgacggagc gctgcacaac
     4561 gtttcgttcg ttgccggcat cggcatcatc acgatcgtgc acatggtgat cggggaacag
     4621 gtgccgaaaa ccgtcgccat acgccacagt cttgccactt cgcttctcgt tgcgtggccg
     4681 ctgcggatct acaacctggt gttcgcacct ttcgtgtgga tcgtgaaagt gctgaccgcg
     4741 ttcacgcttc gtcttttcgg cattaaaaaa acatcagagc acgacgacgt tcacacggaa
     4801 gaagagctcc ggctcattct caccgaaagc gaagaaggcg gagcgatcaa accttcggag
     4861 cacgatctca tccagaacgt tttcgaattc gacgaccgcg cggtgcggag cattctggtt
     4921 cccgccgtga agatctccgc cgtggatatt gatcttgcgc cgcatgaaat tctcgataaa
     4981 gtggtcgaag aaggctattc gcggatgccg gtttacaagg gaaccatcgg caacatcacg
     5041 ggcatcatct acgcgaaaga cctgctgaag ctcatgaaag agcacaagct gacgcgcgaa
     5101 gcggtggaag gaatggtgcg ccccgcccat tttattccgc agaacaaacg catcaatgag
     5161 ctgctccgcg aattccagac acttcacatc cagatggcga tcgtgaccaa tgaattcggg
     5221 ggaatcgccg ggcttgtgac gatggaagac gtgatcgaag agctcgtggg cgagatccag
     5281 gatgaatacg acgaagaaaa accggtggtg gagaaaaaat ccgaaacgga atttctcgtt
     5341 tccgcgatgg caagcatacc ggacgtgaac gatcacatcc cgatcgcgtt accggaatcg
     5401 ccccactatg aaacggtgag cggcctgatg aattatcttt tcgggcgcat tcccgcggtg
     5461 aatgaaaaga agatcttcgg cggctatgaa tttctcatca ccaaacgcct gcggcacagc
     5521 gtcgagcagg tgaagatgac cgtgaccgac tccggccagt tcaatgccga agaaaaatcc
     5581 tgatcgcttt ccctggtttc acgcgaacag cttccggttg ttttccggga gcgatgaaga
     5641 ggaaatgtat ttttccgctg cgcgttttgc accttccgac cattcgcggt attccgtttc
     5701 atccatttca gaaacgcggt cgatgacggc agcaaatttt tccttttcat ccagggaaat
     5761 cacccatccc gctttttctt tttcaagatc cttccacggt gttttgtcgg aaataaggac
     5821 cggcacgccg cactgcatcg cttccaggat gatgtgcccg aaattttctc cccgcgtggg
     5881 cagcagcagg agatcgtacg cgggaagtgt ttcctcaatt ttgccggaat cgagcgctcc
     5941 cctgaaatcc agctttacat tttccggcaa acgcgccgcc gcttcccggc attgccctgc
     6001 gtattcttcg ttataaaccg gtccgaaaaa atccgcctct gccctgctct tcaccatcga
     6061 caaaagctca accgcataca acgtgttttt ttccggcgct atgcgcgcca cgttcacaat
     6121 acgcaccgca cccttctttt tttcgcggcc ggcccccgcg ctcatcgatc tgcggggaag
     6181 attcccggct acgatgatct cattcttttc cccgaaaaac ttcctggcct gctgcgcttc
     6241 ttcttccgtc gttgcgtgaa agatcacacc cgaaaacaaa ttccgcaact tcgcgtacgc
     6301 gaggaacagt tttttcttca gcggctttat cgccagcgcc gacggagcga gcattccgcg
     6361 cacagccacg acgacgcgga catttttctt cattcgtttc aaaacctgca gcggctctat
     6421 cgtaaaatgc ttcgaccaca tcccgttgag gtacacgaca tcgtatgttt ctttttcaag
     6481 aatggaagcg atcgttccgg gcgacaactt gtttccggaa acgtaaaaca cgcgctgtcc
     6541 gccgggaagc ttattccatg catcgctttt tacaccggca taaggaaccg acgaggtgta
     6601 atctgtgtcg cgtgtgacga cggagaaatc gaattcgctc ccgaaatgat cgaggagatt
     6661 ggccacggaa cggacaggac cgccggcttt gtcgccgggg agaaaccagt cgataaagac
     6721 cagcactttc cgtttttcag ctttcgattc catttttagt gagccagtga gaaagcacga
     6781 ccagcggcca gatgcgcgac cacgtcacgc ggggatcgtc gcgcagaaaa gaattccaca
     6841 accggctgat cccttccggg tcgaacggtt cgcgtttacc gagatcttcc aggcgttccg
     6901 cgcaaaaact tttcagctcg ttcttcatcc attttttcca cgggaacgtg aaacccattt
     6961 tcggccggtt gacaatttcc ggcggaagca ggtcgccgag cgaatccacg agcagctttt
     7021 tcggtgtgtg cgggtatttt atttcgtccg ggagactgag cacatattcc accagcctgt
     7081 aatcgaggaa aggaacgcgc acctccagcg catgcgccat gctcatctgg tccgtatcgc
     7141 ggagcagcac attctgcatg tacgttccga tttccgcaac cgaaatacgc gaaagcacct
     7201 ggccgggcga atcgaaatgc cacgaacgcg ttgcgtcggc agccattttt cccggggcag
     7261 gagatcccgt tttccacccg ttcgtgatga gcgcgatctg cccgccggaa agcacctggc
     7321 gggaaatcat gaatgcgttg taaaaatcat acgagctcat ttccatcagc gcggcgatct
     7381 tatcggaaga cacgccgggc ttcatcatct tccggaaatt acccgtgatc ttccgggaaa
     7441 acagcggaag cgaccacaac catttccgtt tttcgagcgc gaccatcctt ttgaatatct
     7501 catagccgcc gaaaagctcg tcaccgccaa ggcccgaaag cgccatcgtt attcccgctt
     7561 cttttgttgc ctttgaaacg acgaacgtat tcggtccgtc gccgctcggg tgatccatcg
     7621 catcgagcgc agcgggaagc tcgtgcagga aatcatcggg cgataatttt atttcatggt
     7681 gctcggtgcc gaatttcttc gcgatcatgc gggcataaac cgcttcgctg aattccgaat
     7741 cgtcgaacgt aacggaaaac gttttcactt tggccgtcgc cacttcactc atcaacgcga
     7801 cgatcgcgcc ggaatcaatt ccgccggaaa gaaaagcgcc gaacggtacg tccgaaacga
     7861 ggcggcattc gaccgcttcg cggagcagct tacgcacggt tgtgcaggct tcggcgtacg
     7921 ttaccggctt ttcacgcggg accaatgccg gccaccagga tgcgctctga atggcgggtc
     7981 cgcgtttttc accttcttca taaaacgtat cctctttccc ggcgagaagg aaacagcccg
     8041 gtttcagcat ggtcacgttc gcaacaatcg aagcgggaca atgaaccgtc tggtaatgca
     8101 ggaaatcgtc gagtccgcgc ccgtatattt ttcgttccgt caacccggaa gccagcagtg
     8161 ctctcacctc ggaagaaaaa ataatcgtcc tgtcgtcctt tatcgtgaaa taaagcggtt
     8221 tgatcccgag acggtcacgc gcaatgaaaa gcgttcgttc gctgtgcgac cagatcgcga
     8281 aggcgaacat gccgttgaag ctgtccaggc atttcgttcc ccattttgcg taagcggcaa
     8341 gaacaacttc cgtatccgaa tgcgtgcgga accggaaccc ttcaccggaa agtccggttt
     8401 tcagctcgcg gaaattatac agctcgccat tgaagatgag cgtatatttt tttcctgcgt
     8461 gctcatactg catgggttga tggccgccgg gagaaagatc gatgatggac aggcgacgat
     8521 gaccgagcgt gatgccttct tcagaaaaaa tcccttcatc gtccgggccg cggtgcgcga
     8581 gcgacgcatt catccgcttc acatgctccc tgtaattttc cttaccgccg ttacaatcga
     8641 atatgccgtt aatcccgcac aagagaaaat agtttttcta aagataatca agtggaagcg
     8701 atgagggcat tcagccggtc tgcccagagc gccggcgtat gcagtgaagc gagacgggcg
     8761 ctttcccctc ccatttccgc aagccgcagc acgggcgatc gcatcgcttc gcgcatggcg
     8821 gatttcagct cgtccctgtt cccgctgttg aacttccatc cgtttttgcc cggttcaagg
     8881 aaaaattccg ccgcaccgac tttgtcgctg aggatcatgg gaaaacctgc ggctgcgtat
     8941 tcatgcacca ccacgcccca gggctcgaac gtgctcggta aaatgaacac accggcccgg
     9001 ctcatcacct cttccatccc ggaagtctgc acaaaaccga ggtgacggac gccttcgatc
     9061 tccggcggaa cgatatcgcc tttcccgagg caccacaatt cccattcacc cggaatttct
     9121 tttttcagct cggcaaaaac ctcccagagc atttccacgt tcttttcttt cgcataacgt
     9181 ccggcgtaaa taaaccgttt cgggaaatgc gcttttttgg cggcttcatg cttttcgaat
     9241 atcccgtgaa aaaaatcgta atcgcagcaa taagcacccg tagcgatttt gtcgcgcggg
     9301 aatcccagct tttcggcgaa cagctcctgc ggtttccccg gaacgaaaca ggcggagaac
     9361 cgcctgcgga aggacacgcg tgcgccctgc gcgagaataa gctgcttcag cgaaccgtcc
     9421 caccagttgt caaacccaag cacgacggga attttcccat ggaaatgccg gctgatggtc
     9481 atgtatggct tgcagcccca tcctgcgcag aagatcgcac cgggcgctgc tccgatagca
     9541 aaatcaaaaa gctctttttc gctgaatgat tcccggtcga cgatcttcac gcgcggtgat
     9601 tcgtgaaaaa caaaaggggc aaccgcattc acttttttcc gcacgatgat cacgtcggcc
     9661 gcatgccgtt cggcaaacgc gtttatgcag gccaggaaat agcccgcaag ctcttcgtaa
     9721 agcaccagta gtttcatccg gaaagaaggc gaaagatatt acttccgcgg aaaagatttt
     9781 agcgcacgct gcatacggat tcttaatttt acgcctgtta cccgcaacag gatggaaatt
     9841 gaagccggca gctacgacaa aagcatgaaa cagcaggtgg ctgcgctctt cagcatgcag
     9901 tatggcgtga gcgaaaaaga atttaccgac ctgatcggca acttctacga acatccgtac
     9961 caggcggaaa aatgcatccg catcgttgcg ctcgacggag aaaaagtcgt cgggttccag
    10021 tcgttcttcc tctggcccta cgaataccag ggcaaggtct ttcgctctta ccagtcggga
    10081 aattcgctcg ttcacccgga ttaccggggc aagggaattt tccagaagct gctccattac
    10141 ctcgaaaaaa agcgggaaga gctcggcgtg gaattcatca tgggttttcc gatcaacgtt
    10201 tcccgcaaca gccttatccg gaacgggtgg aaaaacatcg tcgacctgca atggtacgtg
    10261 aagcccgttc gtttccttcc tgttttcggt ggcaacgaag acgccctgct ccggcgctgt
    10321 ttttccgctg aagctcccgc tcttccatcg cagccggcgc aggaacatat ccgcctgacc
    10381 accgacccgt tctttcgtga atggcggaaa aattattcca cggcaaccgg ccattattac
    10441 tggtttgctt acgaagagaa agatcgcaaa gcggttttcc agctcaagct gatccggcgg
    10501 aaaaagctga tccgggaact tatcgtcggg gacgtgatcg cgcaggacaa cagccgtgct
    10561 tttcttgccg cggcatttac cgcgctggcc aaggcggcgc gtaaaacgcg ttgtgtgcac
    10621 cttatttcat tcgccgtgaa tcccgcgagc ccgtatatca gccacgcaac gcttgcagat
    10681 gccgggatga acacgatcga ccgccacatt ttcttcgcag taaaaccgtt cgctgaaatt
    10741 cccgccctct ccgatccgtc gaaatggatc cttttccggt cggacataga cacgtggtaa
    10801 atgaaatacg acccgaaaat ccatagctgg aggcacctgg tgaaaagcgc gctggggcgg
    10861 atggaattcc tgctcaccgg tgagccctgt ccgccgggcg aactgctggt gctgaactat
    10921 cacggcaccc cgctgaagtt catggacaat ttccgcgaac agatccggtt cttccgcgat
    10981 cgtttcgatt tcatttcacc tgcgcagctc ggtgattatt tttccggaaa gccggacccg
    11041 aaaaaatcat acgcgcttat tacgttcgac gacgggctgc gcaacaacct tcatgcagct
    11101 gaggtcctaa gggaatttaa tatccgcgct tactttttta tcgttccggc gttcatcaac
    11161 gccccggcgg gagatcagaa aaactattac ctgcagcaca tccgtccgca gatcaaccct
    11221 gccatcgatt cccgtgaaga ggatcttctc ccgctttcgt gggaagacgt ccgtcgattg
    11281 cattcggaag gtcacggcat cggcgcgcat tcgctcacgc acgacctgct tgcgtcgggc
    11341 agcgaagaag aaagccggaa agagatcatt tcctgccgcg aagtcattta tgaaaaaacc
    11401 ggcgtgatgc cggattcatt ctgcagcccg aacaacagct tgctcagtgc gggaaaaaaa
    11461 gaaatgcagc tgatccgaga aaattaccgg tatcatttct ccacgcttcc gggaagcaac
    11521 accggcgctt cgcctttttt tatccgaagg gtgaacgtgg aaagcttctg gctgaaagga
    11581 gcggtaatgc gcgccacggg gaaatgggac cgcaaaagat ggacgtcgcg cattatgcag
    11641 ttcagcgcag ttgttaacgg aaactgatca tgcattagcc ggcagggctt ccgtttcgcc
    11701 gtccacttct tcttcttccc cgtcaaaagc ctcttcttca cgcattgcca tttttttacg
    11761 gtattccacg gcgttgaaat aatacatcca cataaagaag ggaaagccgt tcacaaaata
    11821 atttccctgg cggagcaggt agagcaggat gatcgtcatc atcgcagagg agatcagcca
    11881 gtgcgtgttg ttgcgcaact ccccggtgcg gcgcacgaaa ttccttcgca gaaaaacaaa
    11941 catgaaaacg agaccgctca gcccggtttc tgacatcagc cttacaaaca tgctgttccc
    12001 gtcgctctgg ttgaacgtga aattgaggat gtcctcgttt ttcgtaagcg aatatttgtc
    12061 gaatgccgcc tggtgcgagc cgagacccgt accgaaaaga aagttgctct tgaagttttc
    12121 ccaggccacg tggaaattgt tgtacagcac gaagctcgaa gagttgacgt tttcgagcgc
    12181 gtagttctgg taaatccaga gttcgatcgt cgaatcgatc cgggattgaa actccggcac
    12241 aagccggtac agcaggaaca cggcaaccgc gccaaaaaca agcgcagaca cgaaattggc
    12301 gatctgcccg aagttgacag cgagcagcag gatgatgatg aagagcccga aataccccgt
    12361 ggaagagaag gtcaggatga aaacgccgat aacgatcagc gacctcaaaa ccgtgtaata
    12421 tgaagtgtcg cggaagaaaa tactgtaaaa accgaccatg gccgccgggg aaagcacaat
    12481 ggcgcactgc gccggttcgg aaaaaacaga attgatgcgt attccgagga cgctcccggg
    12541 aggaagcccc catttattga acagccagcg gaaattataa agcggtgtga tgtgcaggcg
    12601 gaaagcaacg aactggacga cgccgatcac ggccgcgtaa taggctcctt tgaggtacat
    12661 gcggaaaaga aaatcgacat caaagtcgta atgcctgaaa acgtaatagt aaaaggaagc
    12721 cgacaacacg aggccgataa agatcttgat gaaaaggaac agcgtattgt tatcgctgag
    12781 cacaccgaaa agacccacag caataagaaa gagaagcatg aacc
//
LOCUS       DASTSF010000213         8380 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_707687,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000213 DASTSF010000000
VERSION     DASTSF010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 8380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8380
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_707687"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..515
                     /locus_tag="VFU15_06235"
     CDS             <1..515
                     /locus_tag="VFU15_06235"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEU4717408.1"
                     /translation="GLKEGNHEYRMEVNDAFFESLPFSEIRQGQVDVKVDVAKHSRMM
                     TLSISMEGFVFLPCDRCSADYRQPVSGERKMVVNLEADQFDDEDDLLSLPASVHELDL
                     SQYLYEYISLLVPSRRVCGETPDPSKGCDPEIIAALEKLKPGKEIKSKKKKKEVDPRW
                     DNLKKIKFKN"
     gene            537..722
                     /gene="rpmF"
                     /locus_tag="VFU15_06240"
     CDS             537..722
                     /gene="rpmF"
                     /locus_tag="VFU15_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008465217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEU4717409.1"
                     /translation="MPNPKHRHSKSRRDKRRTHYKAEAPNVAICSVTGEAHLWHRAYW
                     HDGALYYKGKVVMEKKD"
     gene            738..1673
                     /gene="plsX"
                     /locus_tag="VFU15_06245"
     CDS             738..1673
                     /gene="plsX"
                     /locus_tag="VFU15_06245"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011585009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEU4717410.1"
                     /translation="MNIGIDILGGDFAPESPLEGVLLALGELPPSARLVLIGDQEHIS
                     SWLREKNADLSRVDIVHATKVIGMGEHPVKAFSSKPDSTIATGFRLLAEGKIDSFASC
                     GNSGAMLVGSMYTVKTIPGIIRPCITTAFPKEAGGFNLLLDVGTNADCKPDVLYQFAI
                     LGSLYASAVFGIKNPKVGLLNIGEEPEKGNLVAQSAHELMKDSKDFNFVGNVEGRHIF
                     GDEADVIVCDGFTGNVVLKFAESFYAAIKRRKIDDEYFNRFNYEIYGGTPILGVNGNV
                     IIGHGISSPLAIKNILLLSMNIINARLPEKIKRAF"
     gene            1679..2677
                     /locus_tag="VFU15_06250"
     CDS             1679..2677
                     /locus_tag="VFU15_06250"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018475676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HEU4717411.1"
                     /translation="MKKTRAAITGVQGWVPDTVLSNADLEKMVDTNDEWIVSRTGIRE
                     RRILKGEGRGTSEICINAVSRLLEKKKIDPLEVDMVIVGTVTPDFFFPSTSNLVCDKL
                     GLKNAWGYDLTAACSGFLYALSTGSQFIETGRYKKVIVVGADKMSSIIDYEDRATCII
                     FGDGGGAVLLEPSEEEVGVMDFVLHVDGSGGQYLNCLGGGSKYPATRETVDKKMHYVH
                     QEGQAVFKHAVANMAEVSVQIMERNKLSAEDIRWLVPHQANKRIVDATANRMGIGSEK
                     VMMNIERYGNTTAGTLPLLLWDYEKQLHKGDNIILSAFGGGFTWGSVYVKWAYNSN"
     gene            2746..3252
                     /gene="accB"
                     /locus_tag="VFU15_06255"
     CDS             2746..3252
                     /gene="accB"
                     /locus_tag="VFU15_06255"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007095831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEU4717412.1"
                     /translation="MKLNEIQDLIKFVAKSGVSEVELETKDVKITIKTGGKGGKNEAP
                     VVVQASVPVQQGPVVQPVFQPVVTQPAAETKAPETKKENVADESRLITIKSPMIGTFY
                     RAPGPDKPIFINVGDEIKPGKVLCIIEAMKLFNEIESEVSGKIVKVLVDDASPVEYDQ
                     PLFLVEPA"
     gene            3345..4685
                     /gene="accC"
                     /locus_tag="VFU15_06260"
     CDS             3345..4685
                     /gene="accC"
                     /locus_tag="VFU15_06260"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013634327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEU4717413.1"
                     /translation="MFKKILVANRGEIALRIIRTCREMGILTVAVYSTADKDSLHVRF
                     ADEAVCIGPPPSRESYLNIASIIAAAEITNADAIHPGYGFLSENSKFSRICQEHGIKF
                     IGATPEQIDAMGDKSNAKDTMKKAGVPCVPGSDGLLTDAESAKAIAAGIGYPVIIKAT
                     AGGGGRGMRIIWKEEELAPNFESASKEAEAAFGNGAMYMEKYIEEPRHIEIQVAGDQY
                     GKACHLSERDCSIQRRHQKLVEETPSPFMTQELRDKMGEAAIKAALAVSYEGVGTVEF
                     LVDKHRNFYFMEMNTRIQVEHPITEEVIDYDLIREQILIAAGVPISGKNYYPQLHAIE
                     CRINAEDPFNNFRPSPGKITVLHTPGGHGVRVDSHIYAGYTIPPNYDSMVAKLITMAQ
                     TREEAIAKMHRALTEFVIEGVKTTIPFHLRLMKDENFLKGNYTTKFLENFDLSE"
     gene            complement(4758..5171)
                     /locus_tag="VFU15_06265"
     CDS             complement(4758..5171)
                     /locus_tag="VFU15_06265"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEU4717414.1"
                     /translation="MMFRKFFLLLLILAGSRYSFAQQSAADSLQKGVECDPRVKSLVE
                     KDMQIKSEVKEKGYRVQIFFGTDKAKAKDEKARFLAKYGTEVHAYEVYEVPNFKIRVG
                     DFRTRLEAYRFLKEIKGDFPTAFIVESDIEFPDSD"
     gene            5442..6731
                     /locus_tag="VFU15_06270"
     CDS             5442..6731
                     /locus_tag="VFU15_06270"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEU4717415.1"
                     /translation="MKSLWQNVRSARFVVLFFLAVSLLFSSNAYGAPPDGKALFKTNC
                     SSCHSASSKKLTGPGLKGVLGRIPSLDWAHDWVHNNNDLVAKGDPRALQLKKENSNAA
                     MTTFPKLTNEEIDAILNYVDPGPDEPTKAVDAGGNGGGATGPAEDEGSPVFLSLIIIV
                     LLIVLIAILRFTKINLRNVVNEKEGLPEEPGLPFLEASRNWINNNKRKVALMGVFLFC
                     VFIVKGWYALKGIGVYAEEVHPDEWVGYHPSQPINFSHKIHAGDNGIQCQYCHAGVNK
                     SKVAGVPSIMVCMNCHRVISETKNPGSKEEIAKIYFAVGWNPDSAKYCNPQHPVEWNK
                     VHVLPDFVFFSHQQHVVVGQLACDNCHGDVKSMTTVEQQRPLTMGWCVNCHRETPVQM
                     NGNGYYTALHEKMKEKYKDQPITVSMVGGIECARCHY"
     gene            6772..>8380
                     /locus_tag="VFU15_06275"
     CDS             6772..>8380
                     /locus_tag="VFU15_06275"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAT-variant-translocated molybdopterin
                     oxidoreductase"
                     /protein_id="HEU4717416.1"
                     /translation="MANEKKYWKGLAQLNNDPKFAEKHQSEFAEYIPVEEFISDKNAM
                     AGSSTSRRDFLKFLGFSTVAATLAACEAPVTKTIPYVTRPEEIIPGVANWYATSYYDG
                     NDYASVLVKTREGRPIKIEGNTLSKISMGGANARVQASVLSLYDSTRLNGPKKKNGSG
                     WTDISWADADAAIGDKLKQSKGIAVLSSSIISPCTRMAISEFLMKYPGSRHVMYDAVS
                     YSGMLKANNSTFGKAFIPTYHFDKADVIVGIGADFLANWISPIEHAHQYGMTRKVGPD
                     KKTMSKHFQFESLLTLTGANADERFPVKPSQLGQVAVGLLAAVGGSVSAPNVMTKEVA
                     RVADALKNAHGKAVVVCGSNDASVQLVVNEINKQIGAYGSTIDTTITDNTHQGDDAAV
                     KKLVDDMGGAIDTVIFYNTNPVYTAPKAAGFADAMKKLSCRISFASHADETASLCDYI
                     LPDHHYLEAWNDHNPRTNSYSIQQPAIRPLFNTRHAQESFLHWSGIKTNYHSYIQRNW
                     QEHMFGMQNTFGDFTAFWNNTVRDGVIE"
BASE COUNT         2179 a         2150 c         2177 g         1874 t
ORIGIN      
        1 gcgggctgaa agaaggcaac catgaatacc ggatggaggt caacgacgcg ttctttgaga
       61 gcctcccgtt cagtgagata cgccaaggtc aggtggacgt taaggtcgat gtggcgaagc
      121 attcccggat gatgacgctc agcatcagca tggaaggttt cgtatttctt ccctgcgacc
      181 gctgcagcgc cgattaccgg cagcctgttt ccggggaacg gaaaatggtg gtgaaccttg
      241 aggccgatca gtttgacgac gaagacgacc tgctgagcct gccggcttcc gttcacgagc
      301 tcgatctttc gcaatacctc tacgaataca tcagcctgct ggtgccttcg cgcagggtat
      361 gcggggaaac gcccgatccg tccaagggtt gcgatcctga aataatcgcc gccctcgaaa
      421 agctgaaacc cggtaaggaa attaaatcca aaaagaaaaa gaaagaggtt gatccccggt
      481 gggataacct gaagaaaata aaattcaaaa actgaatagc ttaataccaa aataaaatgc
      541 caaatccgaa acaccgccat tccaaaagcc gccgcgacaa gcgccgtacg cattacaaag
      601 ctgaagctcc caacgtggct atctgctctg ttactggtga agctcatctc tggcaccgtg
      661 catactggca cgacggagct ctttattaca agggcaaagt cgtgatggaa aagaaggact
      721 aaccttcccg ccattatatg aatatcggga ttgatatcct gggtggtgat ttcgctccgg
      781 aatccccgct cgaaggcgtg ctgctcgcgc tcggagaatt acctccttcc gcgcgtctcg
      841 tgcttatcgg cgaccaggaa cacatttcct cgtggctgcg tgaaaagaac gcagacctgt
      901 cgcgcgtgga tatcgttcac gcaacgaaag tgatcgggat gggagaacat cccgtcaaag
      961 cattttcttc caaaccggat tcgaccattg ccacaggttt ccgcctcctg gccgaaggga
     1021 aaatcgattc tttcgccagc tgcggaaatt ccggcgccat gctcgtcggt tccatgtaca
     1081 cggtaaaaac gattcccggt atcatacgtc cctgcatcac cacggctttc ccgaaagaag
     1141 ccggcggctt caacctgctg ctcgacgtcg ggaccaacgc agactgcaag ccggatgtgc
     1201 tttaccagtt cgcgatcctc ggaagtcttt acgccagcgc cgttttcgga ataaaaaatc
     1261 caaaggtcgg cctgctgaac atcggggagg aacctgaaaa aggaaacctt gtggcgcagt
     1321 ccgcacatga gctgatgaag gactcgaaag acttcaattt cgtcggtaac gtggaaggcc
     1381 gccatatttt cggcgatgag gccgatgtta tcgtgtgcga cggattcacc ggtaacgtcg
     1441 tgctgaaatt cgcggaaagt ttttatgccg ccatcaaaag aaggaagatc gatgacgagt
     1501 attttaaccg gttcaattat gaaatttacg ggggaactcc tattcttggt gtcaacggga
     1561 acgtgatcat cggtcacgga atttcatccc cgctcgctat caagaatatc ctgctgctgt
     1621 caatgaatat catcaacgcc cgcctgcctg aaaaaataaa aagagctttc tgatgacaat
     1681 gaaaaaaaca agagcggcga tcacaggcgt gcagggatgg gttcccgaca cagtcctttc
     1741 caatgccgat cttgaaaaaa tggtcgacac gaatgacgaa tggatcgttt cccggaccgg
     1801 cattcgcgaa agaagaatac tgaaaggcga aggtcgcggg acgtccgaga tatgcatcaa
     1861 tgcagtgagc cgtttgctcg aaaagaaaaa gatcgatccg ctcgaagtgg atatggtgat
     1921 cgtcggaacg gtaacgcccg attttttctt tccgtccacc tccaacctgg tctgcgataa
     1981 gctgggcctg aagaatgcat ggggttacga cctgaccgct gcctgttcgg gctttcttta
     2041 cgcgctttct acaggttcgc agttcatcga aacgggccgg tataaaaaag tgattgtcgt
     2101 cggcgccgat aaaatgtctt ccatcattga ttatgaagac cgcgccacct gcatcatttt
     2161 cggggacggc ggcggcgctg tcctgctcga gccttccgaa gaagaagtgg gcgtaatgga
     2221 tttcgtgctg catgtggacg gttccggcgg acaatacctg aactgcctcg gtggcggatc
     2281 aaaatacccg gccacccgcg agacggtcga caaaaaaatg cattacgttc accaggaagg
     2341 ccaggcggtt ttcaagcatg ccgtggcgaa catggctgag gtttcagtgc aaataatgga
     2401 acgcaacaaa ctgtctgcgg aagacatccg gtggcttgtt ccgcaccagg cgaataaacg
     2461 tatcgtcgat gcgacggcga atcggatggg catcggttcg gaaaaagtga tgatgaacat
     2521 cgaacgttat ggcaatacaa cggcaggaac acttcccctg ctgctgtggg actacgaaaa
     2581 acagctgcac aaaggcgata acattattct ttcggctttc ggcggaggtt ttacgtgggg
     2641 atcggtatat gtaaagtggg catataattc caactgaaca caaccggctt caataggatc
     2701 atttacatta gcaccctcaa tacacattaa tcaaaaacgt acgcgatgaa actcaatgaa
     2761 atccaggatc tgatcaagtt cgtggcaaaa tccggtgtca gcgaggtaga actcgagacc
     2821 aaggatgtca agatcacgat caagacaggc ggaaagggcg gcaagaatga ggctcctgtt
     2881 gttgtgcagg cttctgttcc cgttcagcaa ggccccgtgg ttcagcccgt ctttcagccc
     2941 gtggtaacac agccggccgc tgaaacaaaa gcgcccgaaa cgaaaaagga aaacgtcgca
     3001 gacgaatcca ggctgatcac gatcaaatcg ccgatgatcg gaacatttta ccgcgctccc
     3061 ggacccgaca aaccgatctt tatcaatgtc ggcgacgaga tcaagccggg caaggtcctg
     3121 tgcatcatcg aagccatgaa gcttttcaat gaaattgaaa gtgaagtgag cgggaagatc
     3181 gtcaaggtgc tggtcgatga cgcctcaccg gtggagtacg atcagccgct cttcctcgtg
     3241 gaacccgctt aaacagtacc ggatgttgaa taatggatta tgaagcgatg aattcatgat
     3301 tcaccattca tattcatcat taccagaaga tggcgaaaga aaaaatgttc aagaaaattc
     3361 tcgtggccaa ccgcggggag atcgcgctgc ggatcatccg cacctgtcgc gaaatgggaa
     3421 ttctgacggt tgcggtttat tccaccgccg ataaagattc tctccacgtg cgctttgccg
     3481 atgaggcggt atgcattggt ccgccgccca gccgcgaatc gtatctcaac atcgccagca
     3541 tcattgctgc agccgagatc acgaacgcgg atgcaattca tccgggctac ggattcctca
     3601 gcgaaaactc gaaattctca cgcatctgcc aggagcacgg catcaagttc atcggcgcca
     3661 cgccggaaca gatcgatgca atgggcgaca agtccaatgc aaaagacacg atgaaaaaag
     3721 caggcgtgcc ctgcgtgccc ggcagcgacg gtctgctcac tgacgccgaa tcggcgaaag
     3781 cgattgcggc cggaatcggt tacccggtca tcatcaaagc gacagcaggc ggcggcggcc
     3841 gcggcatgcg catcatctgg aaagaagaag agcttgcacc gaatttcgaa tcggccagca
     3901 aggaagcgga agcggctttc ggcaacggcg cgatgtacat ggaaaaatac atcgaggaac
     3961 ctcgccacat tgaaatacag gtggccggcg accagtacgg gaaagcctgt cacctcagcg
     4021 aacgcgactg ctcgatccag cgccgtcacc agaagcttgt cgaggaaaca ccctcgccgt
     4081 tcatgacgca ggagctgcgc gataaaatgg gtgaggcagc gatcaaggcc gcgcttgcgg
     4141 tcagctatga gggcgtggga acggttgagt tcctcgtcga caagcaccgc aatttttatt
     4201 tcatggaaat gaacacgcgc atccaggtgg agcacccgat cacggaagag gtgatcgatt
     4261 acgacctgat ccgcgagcag atcctcattg ctgccggcgt tcccatttcc ggaaaaaatt
     4321 attacccgca attgcatgcg atcgaatgcc gcatcaacgc ggaagacccg ttcaataatt
     4381 tccggccttc tcccgggaag atcacggttc ttcacacgcc cggcgggcac ggcgtacgtg
     4441 tggattcaca tatctacgca ggttatacga tcccgccgaa ctacgattcc atggtggcga
     4501 agctcattac tatggcgcag acacgcgaag aagccattgc caaaatgcat cgcgcgctga
     4561 cggaattcgt gatcgaaggt gtgaagacga cgatcccgtt ccacctgcgc ctgatgaaag
     4621 atgaaaactt cctgaaagga aactatacaa cgaaattcct cgagaatttc gacctgtcgg
     4681 aatgattttt cattgacaaa ataaaacccc gctgtcgaaa tgataagcgg ggttattttt
     4741 ttgtgcctgc ctgaagttca gtcggaatcg gggaactcga tgtcgctttc cacgataaag
     4801 gcggtaggga aatcaccttt tatttctttc aggaaacggt aggcttcgag gcgtgtgcgg
     4861 aaatccccga cgcgtatttt gaaattcggc acttcgtaca cctcgtaggc gtgaacttcc
     4921 gttccgtatt tcgcaaggaa acgcgctttt tcatctttcg ctttcgcctt gtccgttccg
     4981 aaaaagatct gcacccggta tcctttttcc ttcacttcgc ttttgatctg catgtctttt
     5041 tccacaaggg acttcacgcg cggatcgcac tcgactcctt tctgcaatga gtcggctgca
     5101 gattgctgcg cgaacgaata gcgcgaaccg gcaaggatca gcaggaggag gaaaaattta
     5161 cggaacatca tcaggcaaaa ttaaccaaat tccgggccgc ggttttaaat cgcttcgaca
     5221 agcactccaa aagctacgcc gaccggaaca attttttgga tgaaaaatgg attttccgta
     5281 tgccggtttt ccgagggcga ttaacattta tttagaatca gtataaatta atttccggtt
     5341 aatttttttc cttttcgcgg gaatcatgga agtagctttc tcataaattt gccacgcctt
     5401 atagtgtaag gtaatcactt aaaaacgcca atttccgcta tatgaaaagc ctctggcaaa
     5461 acgttcgaag cgcccgcttt gtcgttctat ttttcctggc agtttccctt ttattcagct
     5521 ctaacgccta cggtgccccg ccggatggaa aggcgctgtt taaaacgaac tgctcttcct
     5581 gtcacagcgc atcgagcaag aagctgaccg gtcccggcct caaaggcgtt ctgggcagga
     5641 ttccgagtct tgactgggct catgactggg ttcacaacaa taatgatctt gttgccaaag
     5701 gcgatccgcg tgcgttgcag ctgaagaagg aaaattcaaa tgccgcaatg acgacatttc
     5761 cgaagcttac caacgaagag attgatgcta ttctgaatta cgtggatccc ggcccggatg
     5821 aacccacgaa agcggtagat gcaggaggaa acggtggcgg agctaccggt cctgccgaag
     5881 atgaaggttc ccctgttttc ctttcgctca tcatcatcgt tcttctcatt gtccttattg
     5941 cgatccttcg tttcacgaag atcaacctgc gcaacgtcgt caatgaaaaa gaaggccttc
     6001 cggaagagcc gggcctgccc ttcctcgaag cgtcacgcaa ctggatcaac aacaacaagc
     6061 gcaaagtggc gctcatgggt gtttttctct tctgcgtttt tattgtaaaa ggatggtacg
     6121 cgttgaaagg cataggtgtt tacgccgaag aagttcaccc ggacgaatgg gttggttatc
     6181 atccttctca gccgatcaat ttctcgcaca agatccacgc cggtgataac gggatccagt
     6241 gccagtactg ccatgcgggt gtgaacaaat cgaaagtggc cggcgttccg tccatcatgg
     6301 tttgtatgaa ctgtcaccgt gtgatcagcg aaacgaaaaa tcccggatca aaagaagaga
     6361 tcgcgaagat ctatttcgca gtgggctgga atcctgattc tgccaaatac tgcaatcccc
     6421 agcatcccgt cgaatggaac aaagttcacg ttctgcccga cttcgtattc ttcagccacc
     6481 agcagcacgt agttgtcggt cagctggcgt gcgataattg ccacggtgat gtgaaaagca
     6541 tgaccacggt cgagcagcag cgcccgctca cgatgggatg gtgtgtcaac tgtcaccgcg
     6601 aaacgccggt gcagatgaat ggaaatggct attacactgc gctccacgaa aaaatgaaag
     6661 agaaatacaa ggaccagccg atcacggtgt ccatggtcgg cgggatcgaa tgtgcccgct
     6721 gccactattg atctttcaat tatttagtaa aggattttga ctgcaaaaaa tatggcgaac
     6781 gaaaaaaaat actggaaagg actcgctcag cttaacaacg atcccaaatt cgctgagaag
     6841 catcagagcg aattcgccga atacattccg gtagaggaat tcatcagcga taaaaacgct
     6901 atggccggtt cgagcaccag ccgccgggat ttcctcaagt tcctcggctt ttcaacggtg
     6961 gcagctacgc tcgctgcctg tgaagcgccg gtcaccaaga cgatcccgta cgttacccgt
     7021 cccgaggaga ttatcccggg cgtggccaac tggtacgcaa cctcgtatta tgatggaaac
     7081 gattatgctt ccgtcctggt caaaacgcgt gaaggccgcc cgatcaagat cgagggtaat
     7141 acgttgtcca aaatttccat gggcggcgcc aacgcccgcg tccaggcttc cgtgctttct
     7201 ctttatgatt caacgcgtct taacgggccg aagaagaaaa acggaagcgg gtggacggat
     7261 atctcctggg cggatgcaga tgctgcgatc ggcgataagc tgaaacagtc gaaaggaatt
     7321 gctgtccttt catccagtat tatttctccg tgcacaagga tggcgatctc ggaatttctc
     7381 atgaaatacc ccggctcccg ccacgtgatg tacgatgccg tcagctattc cgggatgctg
     7441 aaagccaata attccacttt cggaaaagca tttatcccga cgtatcattt tgataaggcg
     7501 gatgtcattg tcggcatcgg tgcggatttc ctggccaact ggatttcccc gatcgaacac
     7561 gcgcaccagt acggaatgac gcgcaaagta ggaccggata aaaagacgat gtcgaagcat
     7621 ttccagttcg agagcctgct gacgcttacg ggtgcaaatg ccgatgagcg tttcccggtg
     7681 aaaccttccc agctgggaca ggttgccgtc ggtcttctcg ctgcggtcgg cggttctgtt
     7741 tctgccccga acgtgatgac gaaggaagtg gcgcgtgttg cggatgcatt gaaaaatgcg
     7801 cacggtaaag cagtggtcgt ttgcggatcg aatgatgcat cggttcagct ggtggtgaac
     7861 gagatcaaca agcagattgg cgcgtacggt tcgacaatcg atactacgat caccgataac
     7921 acacaccagg gtgatgacgc agcggtaaag aagctggtgg atgatatggg cggcgcgatc
     7981 gatactgtta tcttttacaa caccaacccg gtttataccg ctcccaaagc cgccggtttt
     8041 gcggatgcga tgaaaaaact ttcctgccgt atctcattcg cttctcatgc agatgaaacg
     8101 gcatccctgt gcgattacat tctcccggat catcactacc tcgaagcgtg gaatgatcat
     8161 aatccgagaa cgaattcata cagcattcag cagcccgcta tccgcccgct gttcaacacg
     8221 cgccatgcgc aggaatcatt cctccactgg agcggtatca agacgaacta tcattcttac
     8281 atccagagga actggcagga acacatgttc ggcatgcaga acaccttcgg tgatttcacc
     8341 gcgttctgga acaataccgt tcgtgacggg gtgatcgagc
//
LOCUS       DASTSF010000214         2547 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_383163,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000214 DASTSF010000000
VERSION     DASTSF010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2547
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_383163"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1501)
                     /locus_tag="VFU15_06280"
     CDS             complement(<1..1501)
                     /locus_tag="VFU15_06280"
                     /inference="COORDINATES: protein motif:HMM:NF023260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3352 domain-containing protein"
                     /protein_id="HEU4717417.1"
                     /translation="MSPAKKIILLVTGLLVLAAGVFAYFRISKLKAPARDPLDAVPSS
                     AFCVIGADNPHEAWNTLNHGNLVWDALSVTDWAHEVAATAAETDSLLTADPEAAALTR
                     DQPCWISLNCTGADRFDYFISFSLSTASAGDDAIAFLQKHAKENTSAEKTWKQVKIFC
                     FGNKNGQWCAATEQGIVMISGNEELIKSSIDQLEKGATLKNDKTFAQLMDTRENSATA
                     NVFVNYERFEVAVKRLSNESAYERVENIARFASWSELDLSFRPNAVLLNGYTSAPDSA
                     AGFLSVMKGQTPQRIEAAGVLPASTQNFTSFGISNFGLVMDRYDAFLAKEGHSDERRD
                     HLAQLKRNYGYSPADNIGAWLGNEMVQAEIPSPGGGMVTIALFSAANTAQARSSLSSL
                     ELKNDTSATQPTADSSGYVIRKTPVPEMLSATFGPMFSDLAESYYTIINQFVVFAPDE
                     NSLRSVIAANINGTTLERNRSYAVFVTNISSEASITVYVSPSRCENTLRE"
     gene            1745..>2547
                     /locus_tag="VFU15_06285"
     CDS             1745..>2547
                     /locus_tag="VFU15_06285"
                     /inference="COORDINATES: protein motif:HMM:NF020390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HEU4717418.1"
                     /translation="MISESRQMHHLLLRAGFGMTPELEKKFDRKSRQEAVDEIFKDAA
                     IDQDLLYIQKPKTNDKGEVRPLKVVVLILKSQKEKEMLNLAWIDRMAVAKAQLREKMT
                     MFWHNHFATSAPFGWLMQVQHNTLRKYALGSFRDMLHAISVDPAMIIWLNNQQNKKDA
                     PNENFAREVMELFTLGEGNGYTENDIRQAARAFTGWTVNKKGDFEFHPKEHDDGVKEI
                     FGKSGNFDGSDVIDMLLEKPELAVFICRKIYRWFVNSEVDEERVQDLAD"
BASE COUNT          613 a          675 c          739 g          520 t
ORIGIN      
        1 tttcacgcag ggtgttttca caccgggaag gagaaacgta aaccgtgatg ctcgcttcgg
       61 aagaaatgtt cgtcacgaaa acggcgtagg aacggttgcg ttcgagcgtg gtgccgttga
      121 tgttggcggc gatcacggaa cgcagggaat tctcgtcggg tgcgaacacc acgaattgat
      181 tgatgatggt gtaatacgat tccgcgaggt cggagaacat cggtccgaaa gtggcggaaa
      241 gcatttccgg aacgggcgtt ttgcggatca cgtatcccga cgaatccgcg gttggctgag
      301 tagccgacgt gtcgttcttc agctcgaggg aagaaagcga agatcttgcc tgcgctgtat
      361 tcgcggcgga gaaaagcgcg atggtgacca ttcctccgcc cggcgacgga atttccgcct
      421 gcaccatctc gttgcccagc cacgcaccga tgttgtcggc aggcgaatag ccgtaatttc
      481 ttttcagctg tgcaagatga tcgcgtcgct cgtcggaatg tccttctttc gcaaggaacg
      541 catcgtagcg atccatcacc aggccgaaat tgctgatgcc gaaagaggtg aagttctgcg
      601 tcgatgcggg aagcacacct gccgcttcga tgcgctgcgg cgtctgtcct ttcatcaccg
      661 acaggaaacc cgcggcggaa tcaggtgcgg aggtataacc gttgagcagc accgcgttcg
      721 gccggaaaga aagatcgagc tccgaccacg atgcaaaacg cgcgatgttt tccacccgtt
      781 cgtaagcgga ttcattcgaa aggcgtttca ccgcgacctc gaaccgttcg taattcacaa
      841 aaacgttggc cgtggcgctg ttttcacgcg tgtccatcag ctgtgcgaag gtcttgtcgt
      901 tcttcagcgt cgctcctttt tccagctggt cgatggaaga tttgatcagc tcttcgttgc
      961 ccgagatcat cacgatgccc tgctccgtgg ctgcgcacca ttgcccgttc ttattaccga
     1021 agcagaagat cttcacctgc ttccaggttt tttccgctga cgtattttct ttcgcgtgct
     1081 tctgcaggaa agcgatcgca tcgtcgccgg cggaagcggt ggaaagtgaa aatgaaatga
     1141 aataatcgaa gcggtcggcg ccggtgcagt tgagtgagat ccagcagggc tggtccctgg
     1201 tgagtgcggc cgcttccggg tcggccgtga gaagagaatc ggtttccgca gccgtggcgg
     1261 caacttcgtg cgcccagtcc gtcacggaaa gcgcatccca cacgaggttg ccgtggttca
     1321 gcgtattcca ggcttcgtgc gggttgtccg cgccgatcac gcagaatgcg ctggacggaa
     1381 cagcgtcgag cggatcgcgt gcaggagctt tgagcttcga aatacggaaa taggcgaaca
     1441 cgcctgctgc aagaacgagc aggcccgtga caagcagaat aattttcttc gccgggctca
     1501 cctcaggaaa cgattccgaa atttaccttc agggagggcc ggaaagaaca ttccccgcag
     1561 aacttttcaa acggggaatg ttaacggtta aacccggtgt ttccggccgg gtctaaccac
     1621 aacaactgca agtaacaaaa ccgctcgtgg gacggccatt gcagggcaaa agttacagct
     1681 cccggaaaat ttaaatggct tcatctttgc agcagcagaa tcgacgaaaa caaacagcag
     1741 cagtatgatc tccgaatcca gacaaatgca tcatcttctc cttcgcgccg gtttcggcat
     1801 gacgccggag ctggagaaga aattcgaccg gaaatcgcgg caggaagcgg tggacgaaat
     1861 tttcaaagac gccgcgatcg accaggacct gctttacatc caaaagccga agacgaatga
     1921 caaaggcgag gtgcggccgc tgaaggtggt cgtgctcatc ctgaaatcgc agaaggaaaa
     1981 ggaaatgctc aacctcgcgt ggatcgaccg gatggcggtg gcgaaagcgc agctccgtga
     2041 aaagatgacg atgttctggc acaaccattt cgccacatcc gcgccgttcg gctggctcat
     2101 gcaggtgcag cacaacacgc tccgcaaata tgcgctcggc agtttccgcg atatgctgca
     2161 tgcgatctcc gtcgatcccg ccatgatcat ctggctcaac aaccagcaga acaaaaaaga
     2221 tgcgccgaac gaaaattttg cgcgcgaggt aatggaactg ttcacgctcg gcgaaggcaa
     2281 cggttacacg gaaaatgaca tccggcaggc ggcgcgcgca ttcaccggct ggaccgtgaa
     2341 caaaaaagga gatttcgaat ttcacccgaa agagcacgac gacggggtga aagaaatttt
     2401 cgggaagagc gggaacttcg acggcagcga cgtcatcgac atgctgctcg aaaaaccgga
     2461 gctcgccgtt tttatctgcc ggaaaattta ccgctggttc gtgaactccg aagtggatga
     2521 agaacgcgtg caggacctgg cggacca
//
LOCUS       DASTSF010000215         2081 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_293374,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000215 DASTSF010000000
VERSION     DASTSF010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2081
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_293374"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..513)
                     /locus_tag="VFU15_06290"
     CDS             complement(<1..513)
                     /locus_tag="VFU15_06290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717419.1"
                     /translation="MKKFLKPLLLLIVPAALLFSFQQIDKNFIEWNPARPLTWKDFKG
                     TQEKTSPYAAITEWTIYFSYLAQKDSLHTQVTCYFDPAKSKVKHDARKDSLLLRHEQY
                     HFNLAEVYARKFRKALEESAFSFKDANTRAPKLYSQYVSDCKKEQDLYDKETDHSQKT
                     ADQKRWEDKID"
     gene            complement(531..>2081)
                     /gene="hemC"
                     /locus_tag="VFU15_06295"
     CDS             complement(531..>2081)
                     /gene="hemC"
                     /locus_tag="VFU15_06295"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR00212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HEU4717420.1"
                     /translation="IIASRGSELALWQANFVQEELKRIGVVAEIRIYKTQGDAIQDLS
                     FDKLEGKGFFTKEIEEALLKKEADLAVHSHKDLPTESPAGLSVAAVSYRENPKEMILV
                     RREAVDVRKKFRFRQGAVVGTSSARRKSQLLAFRPDIVLKDLRGNVPTRVQKLRDGHY
                     DAILVAAAGIERLGLDLREFHVEEPDVHEFVPAPAQGVLALQTRSDDNELIALLKKLD
                     HEEVKACIRVERKILNLFDGGCQLPLGVHCTKEEDLFKIHAAYAEAWDKPLRILYTET
                     AKPETAPERMVEKLRSVKPGKVYITRDLRDDDFFFNVLRANGCEVNGKALIDFRRIEV
                     KKVPDADWVFFSSKHAVRNFLSQVKLKPGTKIAAVGKATSDELRKYNLRADFIGGGND
                     TRVTAKQFGAVAGRSRVLFPQAKGSLKTVQQLLPAAQITDLVVYETLQRSAENIPAAD
                     VLVFTSPSNVEAFFEKNSVSTQKLVAMGHATGNTLKEKGFHSFWSPDSFDDTGLVRAV
                     MAALAEKK"
BASE COUNT          393 a          583 c          579 g          526 t
ORIGIN      
        1 gtcgatcttg tcttcccacc gtttctggtc ggccgtcttc tgcgaatgat cggtttcctt
       61 atcgtacagg tcctgctctt ttttgcaatc ggaaacatac tggctgtaca gcttcggggc
      121 cctggtgttc gcatccttaa agctgaatgc ggattcttcc agcgccttcc tgaatttgcg
      181 tgcatacact tccgcgaggt tgaaatgata ttgctcgtgg cggagcagca gggaatcttt
      241 ccgtgcgtcg tgcttcactt ttgattttgc gggatcgaaa tagcaggtga cctgcgtatg
      301 caggctgtct ttctgcgcga ggtaggaaaa ataaatggtc cactccgtga tcgcggcgta
      361 gggcgaggtt ttttcctgcg tgcctttgaa atctttccat gtcagcggcc gtgcgggatt
      421 ccattcgatg aaatttttgt cgatctgctg gaacgaaaag aggagcgcag ccggaacgat
      481 gagcagcagg aggggtttca ggaatttttt cattacagaa aaatagagaa ttattttttc
      541 tccgccagcg cggccatcac cgcgcgcacc agcccggtgt cgtcgaagct gtcgggcgac
      601 cagaacgagt ggaagccttt ttccttgagc gtgtttcccg tggcgtggcc catcgcgacc
      661 agcttctgcg tgctgacgga atttttttcg aagaacgctt ccacattcga gggactcgtg
      721 aacacgagca catcggcggc gggtatattt tccgcgctgc gctgcagcgt ttcgtacacg
      781 acgagatccg tgatctgcgc cgcgggcaaa agctgctgca cggttttcag gctgcctttc
      841 gcctgcggaa aaagaactct gctccgtccc gccacagcgc cgaactgttt cgccgtgaca
      901 cgcgtgtcgt ttcccccgcc gatgaaatcc gcgcggaggt tgtatttccg cagctcatcg
      961 gatgtggctt ttcccacggc ggcaatcttc gtgccgggtt tcagcttcac ctgcgaaagg
     1021 aaattccgca cggcatgctt gctggaaaag aacacccagt cggcgtcggg aacttttttc
     1081 acttcgatgc ggcggaaatc gatgagcgct ttcccgttca cctcgcaccc gttcgcgcgc
     1141 agcacgttga agaaaaaatc gtcgtcgcgc agatcgcggg tgatgtaaac ttttccgggt
     1201 ttcaccgacc gcagcttttc caccatgcgc tccggcgctg tttccggttt cgccgtttcc
     1261 gtataaagaa tgcggagcgg tttgtcccac gcttccgcgt aagctgcatg aattttgaac
     1321 aggtcttcct ccttcgtgca atgtacgccg aggggaagct ggcatccgcc gtcgaaaagg
     1381 ttgagtattt tccgctccac gcggatgcac gctttcactt cttcgtggtc gagttttttc
     1441 agaagcgcga tcagctcgtt gtcgtcgctg cgcgtctgca gcgccagcac gccctgcgca
     1501 ggggcgggaa caaattcgtg cacatccggc tcttccacgt ggaattcacg gagatcgagt
     1561 ccgagacgtt cgatgccggc ggctgcgaca aggatcgcgt cgtagtgccc gtcgcggagc
     1621 ttctgcacac gcgtgggaac atttccgcgc agatctttca gcacgatgtc gggacgaaaa
     1681 gcgagcagct gcgattttct ccgcgcagaa gaagtgccga caaccgcgcc ctggcggaaa
     1741 cggaattttt tccgcacatc cacggcttcc ctgcgcacga ggatcatctc tttcgggttt
     1801 tcccggtagg aaacggcggc gacggataat cccgcgggcg attccgtggg aaggtctttg
     1861 tgcgaatgaa cggcaaggtc cgcttctttt ttcagcagcg cttcttcgat ctctttcgtg
     1921 aaaaaccctt ttccttccag cttgtcgaag ctgagatcct ggatcgcgtc gccctgcgtt
     1981 ttgtaaatgc gtatttcggc aacaacgccg atgcgtttca gctcttcctg cacgaaattc
     2041 gcctgccaca gcgcgagctc gctgccacgt gatgcaataa t
//
LOCUS       DASTSF010000216         2409 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1634966,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000216 DASTSF010000000
VERSION     DASTSF010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2409
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1634966"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..995
                     /locus_tag="VFU15_06300"
     CDS             <1..995
                     /locus_tag="VFU15_06300"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEU4717421.1"
                     /translation="CNSACVRDKKTKKDVCNEEANGSETYVMGLHKTEGNLEVAKREN
                     DAAMYEDNYQQKYNMAISSDEATIILSAYQNLYLEQSMRFAQLCQQQFSTKAGRDDKG
                     VKQAGFLVLWKTSMPSVLIETGFLSSPSEERFLNSDKGQNYMASSIFRAFRAWKDEIE
                     GTPHKYDDAIENMKPWQPDAGDTSGLKKPVNYVAIMNGTAGAKHTPPPAPDTVKPVPP
                     ADSTPVKNSDPPKDSVVTHPATTGVVYRVQIIASDKPIDANSAQFKGVTDTWHYVQGG
                     VYKYTAGKYLTMADASRRQADLKKQGFDQAFIVAFDKNGNRITIDEAKKQNGGK"
     gene            1051..2064
                     /locus_tag="VFU15_06305"
     CDS             1051..2064
                     /locus_tag="VFU15_06305"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEU4717422.1"
                     /translation="MKYSKELKTGIVVVLAVGLLIYGFNFLKGRDIFSTDMDLYAVYD
                     HVDGLTSNNTVQINGFKVGTVRDIHLDPKTNKLVVHFIITDDNAIITKRSTAMIFSDG
                     LLGYKAIQIAAMSGTEKVKDGDTLIGANESGLKDQVSEMVLPLKKKLEGLVSSVDSVV
                     QIFQAVLNQNARQDLVSSFASIRTSLATFEKTSLRLDSMVATEKVKFSDILTKVQSIS
                     TNLANNNEPLSRAINNFANISDSLAKSRLKTTIDSASAVMARVAVIMDKINTGQGSIG
                     MLVNDKKLYTDLTQSSADLDALLTDMKKYPGRYFSIFGKKDKPKKNQNPNNGGNPPPG
                     NGP"
     gene            2080..>2409
                     /locus_tag="VFU15_06310"
     CDS             2080..>2409
                     /locus_tag="VFU15_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006794957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S oxidoreductase"
                     /protein_id="HEU4717423.1"
                     /translation="MEYAGNIIFLLLFIAGVTWFTINVRKIRRNILIGRDEDRSDHRS
                     ERWKTMMRVALGQSKMVTRPVAGIMHIFVYAGFVIINIEVLEIITDGILGTHRALSFL
                     GPLYTFLI"
BASE COUNT          652 a          672 c          609 g          476 t
ORIGIN      
        1 actgcaattc cgcctgcgta agagataaaa aaacaaaaaa ggacgtctgc aacgaagaag
       61 cgaacggatc ggaaacatac gtgatgggat tgcacaagac ggaaggaaac ctcgaagtgg
      121 ccaagcgcga gaatgacgcc gccatgtacg aggacaatta ccagcagaaa tacaacatgg
      181 ccatcagttc cgatgaagcc accatcatcc tcagcgccta ccagaatctc tacctcgagc
      241 agagcatgcg cttcgcgcag ctgtgccagc agcagttcag cacgaaggcg ggccgcgacg
      301 acaaaggcgt gaagcaggcc gggttcctcg tgctgtggaa aaccagcatg ccatcggtgc
      361 tcatcgaaac aggattcctg tcaagtccct cggaagaacg tttcctcaac agcgataaag
      421 gacagaatta tatggcttca agtattttcc gcgctttccg cgcatggaaa gatgaaatag
      481 aaggcacgcc gcataaatat gacgacgcga tcgagaacat gaagccctgg caacccgacg
      541 cgggcgatac atccggactg aaaaagcccg tgaactacgt tgcgatcatg aacggcacgg
      601 ctggtgcaaa gcatacgcct ccacccgctc ccgatacggt aaaacctgtt ccgcctgctg
      661 attccacgcc cgtgaaaaat agcgatccgc ccaaagattc cgtcgttacg catcctgcaa
      721 cgaccggcgt tgtgtaccgg gtgcagatca tcgcttcgga taagcccatt gacgccaatt
      781 cagcccagtt caaaggcgtg acggatacgt ggcattacgt gcagggcggc gtgtacaaat
      841 acacggcagg taaatacctc accatggccg acgcctcccg gcgccaggca gacctgaaaa
      901 aacagggatt cgaccaggcg ttcattgttg cattcgacaa gaacgggaac cgcatcacca
      961 tcgatgaagc gaagaagcag aacgggggca aataaaagtg ggctccgcct ttatttctta
     1021 tttttgcttt ccctttaaca gcccgctatt ttgaagtaca gtaaagagct taaaaccgga
     1081 atcgtcgtcg tgctggccgt cggcctgctc atttatgggt tcaatttcct gaaaggacgg
     1141 gatatttttt ccaccgacat ggacctttat gccgtctatg atcacgtgga tggacttacc
     1201 tccaacaata cagtccagat caacgggttc aaagtgggaa ccgtgcgtga catccatctc
     1261 gatccgaaaa cgaacaagct cgtcgtgcat ttcatcatta cggacgataa cgcgatcatt
     1321 acgaaacgtt cgacagcgat gatcttcagc gatggactgc tcggttacaa agcgatccag
     1381 atcgctgcca tgtccggaac agaaaaagtg aaggacggcg atacgcttat cggtgccaat
     1441 gaatcgggac tgaaagacca ggtgagcgag atggtgctgc cgctgaagaa aaagctggaa
     1501 ggacttgttt cttccgtcga ttccgtcgtg cagatcttcc aggccgtgct caaccagaat
     1561 gcgcgccagg acctcgtatc gagcttcgcc agcatacgca cttcgctcgc cacgttcgag
     1621 aaaacctcgc tgcgcctcga ttcgatggtg gccaccgaaa aggtgaaatt ctccgacatc
     1681 ctgacgaaag ttcagtccat cagcaccaac ctcgcgaaca acaacgaacc gttgtcgcgt
     1741 gcgatcaaca acttcgccaa catcagcgac agtctcgcga aatccaggct caaaacaacg
     1801 atcgattccg ccagcgccgt gatggcccgc gtcgccgtca tcatggacaa gatcaacacg
     1861 ggacagggct ccatcggcat gctcgtgaac gataaaaagc tgtatacgga cctcacgcag
     1921 tccagcgccg acctcgacgc attgctgacg gatatgaaaa aatatcccgg ccgttatttc
     1981 tccattttcg gtaaaaaaga caaaccgaaa aagaaccaga acccgaacaa cggaggcaat
     2041 ccaccgcccg gcaacgggcc ctaaccaggt tcagaacgta tggaatacgc cggcaacatt
     2101 atttttcttc ttcttttcat cgcgggtgtg acgtggttca ccatcaatgt ccggaagatc
     2161 cgcaggaaca ttctcatcgg ccgcgacgag gaccgcagcg atcacagatc cgaacgctgg
     2221 aaaacgatga tgcgcgtcgc cctcgggcaa tcgaaaatgg tgacgcgtcc cgttgccggc
     2281 atcatgcaca ttttcgtgta cgccggtttt gtcatcatca acatcgaggt gctggagatc
     2341 atcaccgacg ggattttggg aacgcaccgg gcgttgtcgt ttctcggacc gctgtacacg
     2401 ttcctcatc
//
LOCUS       DASTSF010000217         4315 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_933589,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000217 DASTSF010000000
VERSION     DASTSF010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4315
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_933589"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..543
                     /locus_tag="VFU15_06315"
     CDS             <1..543
                     /locus_tag="VFU15_06315"
                     /inference="COORDINATES: protein motif:HMM:NF018770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1365 domain-containing protein"
                     /protein_id="HEU4717424.1"
                     /translation="KNGMEKPPARVLLITNLRLLGYVFNPVSFYFCYGEKGEPLCAVA
                     EVSNTFREMKLYYIGKENFDGRKFHLRVPKYFYVSPFIDHDAEFDFSLELPGEKMNIR
                     IDDYKDGNRFFISTLTGEKKALTTGRLVAYFFRFPFITLKIIFLIHWNAMVLWLKKIR
                     YFRKGELKELQRDVMREYKG"
     gene            945..1961
                     /locus_tag="VFU15_06320"
     CDS             945..1961
                     /locus_tag="VFU15_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002700946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEU4717425.1"
                     /translation="MFGIVHIKFDSMTYVLHFRNGAIKREGRGLSFFYFVPNSSIVAI
                     PMGSNDLPFIFNESTNDYQTVSIQGQISYKISKPKMLADVLDFTVSDNGKYKKNDIEK
                     LNQRIINEAQTATSSFIHGIKLKEAIRSAKAIEEKILEGLKSSSAISLLGVEILGANI
                     LAVKATPEMARALETETREKLQQEADQAIYERRNFAVEQERKIKESELNTEIAVEEKQ
                     KQIAEKKMESEVQKAENDRKLREMKLDADIAVENQRKQLIGQKTENDRKEADTQGYVI
                     ATTLKPYKEIDWKTLTALNNNSDPRFNISLAFRELAENAQKIAHINISPELLDSILND
                     KKQQ"
     gene            1958..2911
                     /locus_tag="VFU15_06325"
     CDS             1958..2911
                     /locus_tag="VFU15_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016170105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEU4717426.1"
                     /translation="MNVEYAIIVRNKTRLETLIERFNTKAQAKFYIERLGGNFADYEE
                     EDETFHDSLNALQARLSKIIRHKVVDRAYVPSFIFSEKNVIVVIGQDGLVANTAKYSG
                     GVPIVAVNPDKARYDGILLPFDVSDFDAAVESVMSGKFHSRTMHFAEARLNDGQRLLA
                     FNDLFIGASTHVSARYRISYNDRTEEQSSSGLIVSTPAGSTGWLSSVFNMAYGVTGLF
                     EKNLRPKQPDLHENELLFAVREPFRSVRTQTGIVAGLITGRDRLNIESLMPSGGVIFS
                     DGIETDFLKFNSGSIAQIGIARETALLVTNGKKAPASKKNR"
     gene            3024..>4315
                     /locus_tag="VFU15_06330"
     CDS             3024..>4315
                     /locus_tag="VFU15_06330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717427.1"
                     /translation="MKRFLFLLFLLPLASCFHKDVMEDRADDVGVAIFYSGAEGKMII
                     TREDVFQVQSKETNGSVTNFTGYNECRISSYDLATGKLLSRVNMGHGIEKAFVILGCT
                     PGKLWVYSIDPALGLHCRNPKTLDVIQNEAALSASGPLKNFDFARPDWTKLKENYGWK
                     ARNGLVMITDMQGYHYYYDPDKNTLEKTENDIHDYSLAVNYLSVSGKFSDERYFSLSS
                     GSRRKLMLHEEDSTARFSYLSGQFVIDNDPRHEAARKKTASDSLQRIATALNDSLAIL
                     FAHFPTLKNDVLMFQAKTPEEHEALDQVRALQRRVDQATREVKRISRERTRYPEDALL
                     SDGPGNFFILHASNISDTAKMLLTKVHYGKDGFAEMWHLRLNDFFFTPAKAYQEGAFG
                     EEFDKGNPQFGFQWFDCEDGKIVLISQLEMTCIDAATGR"
BASE COUNT         1069 a         1097 c         1169 g          980 t
ORIGIN      
        1 aagaacggga tggaaaaacc gcctgcgcgt gtgctgctca ttaccaacct ccgcctgctg
       61 ggctatgtat tcaacccggt ttccttttat ttctgttacg gcgaaaaggg cgaaccgttg
      121 tgtgccgtgg cggaagtgtc gaacacgttc cgggagatga aactgtatta tatcgggaaa
      181 gaaaatttcg acgggagaaa atttcacctg cgcgtgccga agtatttcta cgtttcaccc
      241 tttatcgacc acgatgcgga attcgatttc agcctggagc ttcccgggga gaaaatgaac
      301 atccgcattg acgattacaa ggacggcaac cggtttttca tcagcacgct caccggcgaa
      361 aagaaagcgc tgacgacggg aaggctcgtc gcttattttt tccgtttccc gttcatcacg
      421 ctgaaaatta ttttcctcat tcactggaac gcgatggtgc tgtggctgaa aaagatccgc
      481 tatttccgga aaggagagct gaaggaactg cagcgggacg tgatgcggga gtataagggg
      541 tagttcccgg tttcccgttt gccgcgtgtt ccttgtgttg caaaagaaat tcccgcaaag
      601 tcggtaagct gcatgagaaa aaactgcagt cggggcgaag cggaaacagt ttggagtccc
      661 ggatttattc ctgcccggat tttctttatg ccttcgtggt taaaacggtt tcaggtttcg
      721 ggtttagttt gaatgattta tgttttcctt cgtgcgcttt gtgaaaatgc tttgtgttct
      781 tcgtggtttt gactttctaa ctttcaactt tccaactttt cgatcttttc aattttccag
      841 cttgtcaatt ttgaacccgg aacgcggacc tggaatctga acaatacgct gtttccgccg
      901 ttttgtaact tgcgtctgca attcatcatt caaaacctca ttccatgttc ggcattgtcc
      961 acatcaagtt cgattccatg acgtacgttc ttcatttcag gaacggtgcg atcaaacggg
     1021 aaggccgcgg gctttccttt ttctattttg tacccaacag ctccatcgtt gccattccca
     1081 tgggcagcaa cgatcttccg ttcatcttca acgaatcgac gaacgattac cagaccgttt
     1141 cgatccaggg acagatcagt tacaagatca gcaagccgaa aatgctggcg gacgtgctcg
     1201 atttcacggt gagcgataac gggaaataca agaagaacga catcgagaag ctgaaccagc
     1261 gcatcatcaa tgaagcgcag acagccacgt cttccttcat tcatggcatc aagctcaaag
     1321 aagccatccg ttcagccaaa gcgatagagg aaaaaattct tgagggactg aaatcatcct
     1381 cggccatcag cctgctgggg gttgagatcc tcggcgcgaa tattctcgcg gtgaaagcca
     1441 cgccggaaat ggcgcgcgca ctggaaacgg aaacgcgcga aaagctgcag caggaggccg
     1501 accaggcgat ttacgaaaga aggaatttcg ccgtggagca ggagcgtaag atcaaggagt
     1561 cggaactgaa caccgagatc gccgtagagg aaaagcaaaa acagatcgcc gagaagaaaa
     1621 tggaatcgga ggtgcagaag gccgagaacg accgcaagct gcgcgagatg aagctggacg
     1681 cggatatcgc cgtcgagaac cagcgcaaac agctgatcgg gcagaaaacg gaaaacgacc
     1741 gcaaggaagc cgatacacag ggctacgtga tcgcaacgac gctgaaacca tacaaggaga
     1801 tcgactggaa aacgctcacc gcgctcaata ataattccga tccgaggttc aatatttcgc
     1861 tcgccttccg cgagctggct gaaaacgcgc agaagatcgc ccacatcaat atcagtcccg
     1921 agctgctgga ttccatcctg aacgacaaga aacagcaatg aatgtcgagt acgcgatcat
     1981 cgtccggaac aagacacggc tcgaaacgct gatcgaacgt tttaatacga aagcgcaggc
     2041 gaaattctac atcgagcgtc tcggcgggaa ctttgcagat tatgaagagg aggatgaaac
     2101 gttccatgat tcgctgaatg cgctgcaggc ccgtctttcg aagatcatca ggcataaggt
     2161 cgtcgaccgt gcgtatgtgc cttcctttat tttttcggaa aagaacgtca ttgtcgtgat
     2221 cgggcaggac gggcttgtcg cgaacacggc gaaatattcg ggcggcgtgc ccatcgtcgc
     2281 ggtaaatccc gacaaggcgc gttacgacgg catcctcctt ccgttcgacg tgtccgattt
     2341 cgatgcggcc gttgaaagtg tgatgagcgg gaaattccat tcgcgcacga tgcattttgc
     2401 agaagcaaga ctgaacgacg gtcagcgact gctcgctttc aatgatcttt tcatcggcgc
     2461 ttcgacgcat gtttcggcgc gttaccgcat ttcctataat gacaggacgg aagaacagtc
     2521 gtccagcggt ctcatcgttt ccacgccggc cggcagcacg ggctggctga gctcggtatt
     2581 caatatggct tatggggtta ccggtttgtt cgaaaagaac ctgcggccga aacaacccga
     2641 cctgcatgag aatgaattgc ttttcgccgt gcgggaaccg ttccgcagtg tgcgcacaca
     2701 gacgggaatc gtggcaggtc tcattaccgg gcgcgacagg ctcaacatcg aatcgctcat
     2761 gccttcgggc ggtgttattt tcagcgacgg catcgaaacg gatttcctga aattcaacag
     2821 cggttccatc gcacagatcg gcattgcccg cgaaaccgca ttgctggtga cgaacgggaa
     2881 gaaggcccct gcttcaaaaa agaacaggta gccggcgggc gtgggaaacg cctgctgaaa
     2941 agcaaatacg ggtggtgatc atccccctcg tcaatatcgt ttcgtggcgg aacttcccgt
     3001 ttcgtaactt gcatgcccaa ctcatgaaac ggtttctttt cctgcttttt cttcttccgc
     3061 tggcgtcatg ctttcacaag gacgtgatgg aagaccgtgc cgacgatgtc ggtgtggcga
     3121 tcttctattc gggagcggaa ggaaaaatga tcattacccg cgaagatgtt ttccaggtgc
     3181 agagcaagga gacgaacggc agcgtcacga atttcacagg ctataacgaa tgccgtattt
     3241 cctcctatga cctggccacg ggaaagctgc tttcgcgcgt gaacatgggg cacggcatcg
     3301 aaaaagcatt tgtgatcctc gggtgcacgc ccgggaaatt gtgggtgtac agcatcgatc
     3361 ccgcgctcgg tcttcattgc cgcaacccga aaacgctgga cgtcatccag aacgaggcgg
     3421 ccctgtctgc ttcgggtccg ctgaagaatt tcgacttcgc ccgccccgac tggacgaaat
     3481 tgaaagagaa ctacggctgg aaggcgagga acgggctggt gatgatcacg gacatgcagg
     3541 gctatcatta ttattacgat ccggataaaa acacgctgga gaaaacggag aacgatatcc
     3601 atgattacag tctcgccgtc aattaccttt ccgtttccgg gaaattttcc gacgagcgct
     3661 atttttccct ttcgtccggc agccgccgca agctgatgct gcacgaggaa gattccacgg
     3721 cgagattttc atacctctcg ggccagttcg tgatcgacaa cgatccgcgg cacgaggcgg
     3781 cgcgcaagaa gaccgcttcc gacagcctgc aacgcattgc caccgcgctc aacgattcgc
     3841 tggcgatatt gtttgcacat ttccccacgc tgaaaaacga tgtgctcatg ttccaggcga
     3901 aaacgccgga ggagcacgag gcgcttgacc aggtgcgcgc gctgcagcgg agggtggacc
     3961 aggcaacgcg ggaagtgaag cgcatcagcc gggagcgtac gcgttacccg gaagacgctc
     4021 ttttgtcgga cgggcccggg aattttttca tccttcacgc ttcgaatatt tccgatacgg
     4081 caaaaatgct gctgacgaaa gtgcattacg gtaaagatgg ttttgcggag atgtggcacc
     4141 ttcgtctcaa tgattttttc ttcactcccg ccaaagccta ccaggaaggt gcgttcgggg
     4201 aagaattcga taaaggaaac ccgcagtttg gcttccagtg gttcgattgc gaggacggga
     4261 agatcgtgct catttcccag ctggagatga cctgcatcga tgctgctacg ggacg
//
LOCUS       DASTSF010000218         2457 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1153435,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000218 DASTSF010000000
VERSION     DASTSF010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2457
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1153435"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..201)
                     /locus_tag="VFU15_06335"
     CDS             complement(<1..201)
                     /locus_tag="VFU15_06335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717428.1"
                     /translation="MKNTTKLVMAFIALCAAPKVFAQTPDLNVTFRAHYQYASPHECA
                     NICGYVDSLGNEYALVGEDIGMS"
     gene            523..792
                     /locus_tag="VFU15_06340"
     CDS             523..792
                     /locus_tag="VFU15_06340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717429.1"
                     /translation="MLTSVCRETEPGKEEVTLSAGGLDSAANTHVDWLLRDVSEGDVV
                     TIKIITGSFDPPQKARKKPEELVLENKLKYFYRLKEELKEHLREE"
     gene            892..1296
                     /locus_tag="VFU15_06345"
     CDS             892..1296
                     /locus_tag="VFU15_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006987087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCC motif membrane protein"
                     /protein_id="HEU4717430.1"
                     /translation="MDNQNPPFPPPPPSGGNPFQQSSFPNPGPFGTQNDLPNSTAVLV
                     LGIISIVACLCDGLPGLVCGIIALVLAGKANSLYTENPNAWTLKSYNNLKAGRVCAII
                     GTILSGITMAWVIVELAFFGSALSTMPWQNFH"
     gene            1346..1654
                     /locus_tag="VFU15_06350"
     CDS             1346..1654
                     /locus_tag="VFU15_06350"
                     /inference="COORDINATES: protein motif:HMM:NF022281.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2752 domain-containing protein"
                     /protein_id="HEU4717431.1"
                     /translation="MGLADWLENHMLSCPYRSLTGFDCPGCGFQRSLVLLLRGEFFES
                     VHLYPALIPILSTFVFLFIHLIFKFEKGGIIVKWLFIFSVAVIVVSYVIHMTHIYHHA
                     "
     gene            1677..2045
                     /locus_tag="VFU15_06355"
     CDS             1677..2045
                     /locus_tag="VFU15_06355"
                     /inference="COORDINATES: protein motif:HMM:NF040945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCC motif membrane protein"
                     /protein_id="HEU4717432.1"
                     /translation="MIPPFPPDNPGNQFRKPPGANIPYHQPRELPNASSSLVMGILSI
                     IGALCLGVPGIALGVIAMIMGGNAVKNAENNPGAFRESDIRNAKAGKVCALTGTLLGA
                     LILLFIICLAITREDPFHSF"
     gene            2071..>2457
                     /locus_tag="VFU15_06360"
     CDS             2071..>2457
                     /locus_tag="VFU15_06360"
                     /inference="COORDINATES: protein motif:HMM:NF015791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HEU4717433.1"
                     /translation="MKILSTAQTRAADQYTISHEPVSSVDLMERAAGKCTDWIAARYG
                     NENPVAVFCGNGNNGGDGLAIARLLHERKYNVSVYVVRPSAKDSADFSVNLERLKKCG
                     VVATELAKENDISALKSDHRIVIDAVF"
BASE COUNT          610 a          671 c          604 g          572 t
ORIGIN      
        1 ggacatgccg atgtcctcac ccacgagcgc atattcattt cccagcgaat ccacgtaacc
       61 gcagatattg gcgcattcgt gcggcgatgc atactggtaa tgtgcgcgga acgtgacatt
      121 gagatcaggc gtctgtgcaa acactttcgg tgctgcacac aacgcgatga aagccattac
      181 aagcttcgta gtatttttca tgcttaaaga tgaagtttgt taattttcgg agggacgttg
      241 atcatcaaag aaaagcaaaa aaaacggggc aatcaaggcc gttaaacaga gtttatcaga
      301 cggaacggcc ttcccggata acaggtaaat tgcaggaatg aaattcacag acctgcgacc
      361 tgacccgctg ctgcgtttcg gcaaatccgg cgggcagatc agacgacatg aaacctgtac
      421 caaacttttt accgaaccat gagctacgga ttcgaaattg cgcttaacgg cgaaacactt
      481 tgccgtgccg gcatcgatga gaactaatac gtaaccacct gcatgttaac ttctgtctgc
      541 cgggaaactg agccgggaaa agaggaagtt acgctcagcg caggcggact tgattccgct
      601 gcaaatacgc atgttgactg gctgctcagg gacgtctcgg aaggagatgt ggtcactata
      661 aaaatcatca cgggcagttt cgatccgccg cagaaggcca ggaagaagcc cgaagaactg
      721 gtgcttgaaa acaagctgaa gtatttctac cggctgaagg aggaattgaa agaacacctg
      781 cgggaagaat aacaaaagcc gccggtctca aattatttcc ccctggcttt cccctttcga
      841 tgatttttat accttagacg gcaacaaaaa ccaaacaaca caacacacct tatggacaat
      901 caaaacccac catttccgcc tccgccgcct tcgggaggaa atccttttca gcaatcttcg
      961 tttcccaacc cgggaccttt cggcacgcag aatgacctgc cgaattcgac ggccgttctt
     1021 gtgctgggca tcatttccat cgtagcctgt ctttgcgacg gtttgcccgg acttgtctgc
     1081 ggtatcatcg cactcgtgct tgccggaaaa gcgaacagcc tgtacacgga aaacccgaac
     1141 gcgtggacgt tgaaatccta caacaacctg aaagcaggcc gcgtgtgcgc gatcatcggg
     1201 accattcttt ccggtatcac gatggcgtgg gttatcgtag agctcgcctt cttcggatcg
     1261 gcgctgagca ctatgccctg gcagaatttc cattaatccg atcaaaaact gaaggccgga
     1321 aattttccgg ccttttttta tttccatggg actcgccgac tggctcgaaa accacatgct
     1381 gtcgtgcccg tacagatcac tgacgggatt cgactgtccc ggctgcggat tccagcgttc
     1441 gcttgttctt cttcttcgcg gcgagttttt cgaaagcgtt catctctacc cggcgctcat
     1501 tcccatcctg tccacatttg ttttcctctt cattcatctt attttcaaat ttgaaaaagg
     1561 cggcatcatc gtgaaatggc tttttatttt ttccgtggcc gtgatcgtcg tcagctatgt
     1621 cattcacatg acgcacattt atcaccacgc ttgatattgt tttgtaattt tctccgatga
     1681 tacctccgtt tccacccgac aacccgggaa atcaattccg gaaaccgccc ggcgcgaaca
     1741 ttccgtatca ccagcccagg gagctgccca acgcaagttc ctcgctcgtt atgggaatac
     1801 tttccattat cggcgcactt tgcctcggcg tccccggtat agcgctcggt gtaattgcga
     1861 tgatcatggg cgggaacgcg gtgaagaacg ccgaaaacaa cccgggcgca ttccgcgaat
     1921 ccgacatccg caacgcgaag gccggaaaag tatgcgcact caccgggacg ttgctcggcg
     1981 cactgatcct gttgttcatc atctgcctcg cgatcacgag ggaagacccg ttccattctt
     2041 tctgacacga ttaaaagcgc aaacgcttcc atgaaaatac tcagcacagc acagaccagg
     2101 gccgccgatc agtacacgat atcgcacgaa ccggtaagtt cggtcgacct tatggaacgc
     2161 gctgccggga aatgcaccga ctggatcgcc gcacgttacg ggaacgaaaa cccggtggct
     2221 gtcttctgcg gcaacgggaa caacgggggc gacggtcttg cgatcgcccg cctgctgcat
     2281 gaacggaaat acaatgtgag tgtttatgtc gtgcgtccct ccgccaaaga ttctgcggat
     2341 ttttccgtga atcttgagcg actgaaaaaa tgcggcgttg tggcaacaga gctggcgaag
     2401 gaaaatgaca tctccgcgct gaagagcgat caccgcatcg tgatagacgc cgtcttc
//
LOCUS       DASTSF010000219         7893 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_49959,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000219 DASTSF010000000
VERSION     DASTSF010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 7893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7893
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49959"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1323
                     /locus_tag="VFU15_06365"
     CDS             <1..1323
                     /locus_tag="VFU15_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013454115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU4717434.1"
                     /translation="LFMSVVVALFIGLTVSAEEIIRDRKILKRESFLNLSKTSYLFSK
                     ISIMFILSAIQTATFVLVGNLVLGIKGMWVDYWLVLFTTSCFANMLGLNISSAFNSAV
                     TIYILIPFLIIPQLLLSGVIVKFEKLNPTITSQKNVPVAGNMMASRWAFEALAVDQFI
                     DNKFERHFYAYDEMMSYTDFRKNYWIPHLRGIIDKCETNGKSVTPKGYADAEKGILRT
                     KEDSMANEQMVDVSLLKDEIASDLMRLVDFSTRKPAKIAPLGKQLRAQLDLLADSIAK
                     TKYSAFGTNEANHMRDYLNLVNSVYIKIFNFNSGKRDQEVHNLERGGKTAFVDLKNDY
                     YNEGLGDMVTNKNEFNKLLEQDGILIQRADPVFLEPYQSDIGNAHFYAPRKRFFGTYI
                     STFWFNIGVLWSMSLLMFITLYFDLLRKIIDGLGSLGERFGKKKDQPK"
     gene            complement(1375..2568)
                     /locus_tag="VFU15_06370"
     CDS             complement(1375..2568)
                     /locus_tag="VFU15_06370"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015808385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEU4717435.1"
                     /translation="MTHLSDRISRLSESQTIAMARKSRELIAKGIDIISLSLGEPDFK
                     TPDVIKKAAEEAIEHDFSYYTHVSGYLELREAICEKFRRDNGLAYSPDQVMVSSGAKQ
                     AIANAVLSLVNPGDEVIVPSPYWVSYLEIIKLAEGVPVIIKSSIENDFKVTAEQIAKA
                     ITPKTRLMIFSTPCNPTGSVYSKEELRGIAEVIAKQPECYIISDEIYEHINFAGGLES
                     MAQFDFIYDRTVTINGVSKGFAMTGWRGGILAAPKWIAQACDKMQGQFTSATSSITQK
                     AMHAAMLMDPATTHPMRDAFRRRRDLMLAGMKEIPGLKLNVPDGAFYLFPEVSAYFGK
                     KYGDHVIGNATDLSLYLLDQAHVAIVPGAAFGDDNYIRISYATSDDKLTEAVRRMKDA
                     LAKLS"
     gene            complement(2664..3272)
                     /locus_tag="VFU15_06375"
     CDS             complement(2664..3272)
                     /locus_tag="VFU15_06375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717436.1"
                     /translation="MTLTVTALGLLQQSYEPSQVYHDSLGYALIPGPNSSPVSYFTVR
                     TYDDPAQGISSTPITRNEFLAIASGWGTSSVNPKNENLFAKYGVQNCGYFPDTIIRHV
                     LYKGGVACSPVDNLWRLYCGEYPFFIQSPVADTLHKQDIQDPGVSDAGWANDTLRPSD
                     GQIGILQGYGVRFFTDIIYGENAFHLLHDMQDPAWVSNYKSH"
     gene            3442..3942
                     /locus_tag="VFU15_06380"
     CDS             3442..3942
                     /locus_tag="VFU15_06380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717437.1"
                     /translation="MKYILPSLVVFAGCYLIVKNFLETETKRRLAEIRLANQNVVTPM
                     RLQAYERIAIFLERINPNTLITRTHKSGMSARLLQSELTKVIRTEFEHNLSQQIYMSN
                     HAWEMVKTAKEEVTKLVNVAASKLPDTATGVDLSQMILQISQQIEKLPTQVALEYIKK
                     EVGQVF"
     gene            3987..5558
                     /locus_tag="VFU15_06385"
     CDS             3987..5558
                     /locus_tag="VFU15_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012269216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEU4717438.1"
                     /translation="MNKKTEEQQKKFCTDSGIEIKRVYPKKDIPEEQPGEFPFTRGVQ
                     PDMYRGKLWTMRQYAGFSTAEESNKRYHYLLKNGTTGLSVAFDLPTQIGYDSDHAFSE
                     GEVGKSGVAIDSLADIEILFDGIKLEDVTTSMTINSTASILLSMYIALAKKQGADLKK
                     ISGTIQNDLLKEYAARGTYIYPPKASMRIITDIFEYCSREVPKWNTISISGYHIREAG
                     STAVQELAFTLANGKAYLKAALDKGLDIDVFAKRLSFFFNAHNNFFEEVAKFRAARRM
                     WAKITKELGAKDPRAMMLRFHTQTGGSTLTAQQPQNNIIRVTLQALSAVMGGTQSLHT
                     NGFDEALALPTEEAARIALRTQQIIAWESGAPESVDPLAGSWMVEALTDEVEAKAWEY
                     IRQIDAMGGSVAAIEQGFMQDEIAAASYAYQAAIQGGEKIIVGVNRFQVKEKAMENLL
                     TIDDSIRLTQVEKIRKVKATRDNPKVKMLLGKIESAAKGSENLMPGILEAVEAYATLG
                     EIADVMRGVFGEYRQ"
     gene            complement(5641..5946)
                     /locus_tag="VFU15_06390"
     CDS             complement(5641..5946)
                     /locus_tag="VFU15_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013633505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEU4717439.1"
                     /translation="MKFFRKTVSVLSNKYLLTILAFTTWIFFFDRNDVFSQVDRRAEV
                     KKLQQEADYYRNEIANNKSEISELKSNPKELERFAREHYLMKRDGEDIFVVVEDSAK"
     gene            6146..7084
                     /locus_tag="VFU15_06395"
     CDS             6146..7084
                     /locus_tag="VFU15_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008241907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEU4717440.1"
                     /translation="MTLQQLEYASAVAQWGSFVTAAEKCFVTQPTLSMQVKKLEDEAG
                     ITLFDRSRQPVALTDAGKLFIEQARVVLREQKRLKEVIAATKGDIAGTLHIAIIPTVA
                     PYLIPLFAEKFAEKFPDVHMVIEEMTTENIVRALKEEAIDCGILATPLGEHSLNEMPL
                     YNEPFVAYLSRKNTLWNKKQLKAEDIDVTKVWLLADGHCMRTQVLHICAARKEKAGEL
                     RLDYKSGSIETLRRMTDLGEGMTILPWLAVPGLTSKQQEAVRYFRSPEPVREISIVTH
                     RAQLKKALIDKLAESIVSHLPKQLKRHAKQEVVRVY"
     gene            complement(7134..>7893)
                     /locus_tag="VFU15_06400"
     CDS             complement(7134..>7893)
                     /locus_tag="VFU15_06400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717441.1"
                     /translation="GINEMNCPLTMMRSSNTVYVSYPNGVDAFGNTAYSGALARVNKT
                     VIGTGIELRTGLLYLLLSRDKYRFRIADSEIIGFIIGGNKEKRFDYTTKQRLPLPGGL
                     YSSPWIGGSYVLAAGLKACYRINNLIDIGVSYYPLYYYIDIGASITGNTTGAYISGYG
                     GHLRVGKIYADFMEVKLPSKQTDRWDHGPTYNVSVKYRYGSKWHFLSFNSMNQRVNTD
                     VQNNSGLFMPQDVAAKAPVPQKWWVYQVGWGIMF"
BASE COUNT         1917 a         2020 c         2215 g         1741 t
ORIGIN      
        1 cttttcatgt cggtcgtggt ggcgttgttc atcgggctta cggtgagcgc agaggagatc
       61 atccgcgacc ggaaaatact gaagcgtgaa tcgttcctca acctgagcaa aacgagctac
      121 ctcttctcga aaatttccat catgttcatc ctttccgcga tacagacggc gacgttcgtt
      181 ctcgtgggaa atctcgttct cggcatcaaa ggcatgtggg tggattactg gctcgtgctg
      241 ttcaccacgt cctgcttcgc gaatatgctg ggactgaaca tttcttcggc gttcaattcc
      301 gccgtgacga tctacattct cattccgttc ctgatcattc cccagcttct gctcagcggt
      361 gtgatcgtga aattcgaaaa gctcaacccg acgatcacgt cgcagaaaaa cgttcccgtc
      421 gccggcaaca tgatggcctc ccgctgggcg ttcgaagcgc ttgcggtcga ccagttcatc
      481 gacaacaagt tcgagcgcca tttttacgcg tacgatgaga tgatgagcta cacggatttc
      541 cggaaaaatt actggatccc gcacctccgc ggcatcatcg ataaatgcga aacgaacggg
      601 aagtccgtta cgccgaaagg ctatgcagat gcggaaaaag gaattctccg cacgaaggaa
      661 gattccatgg cgaacgagca gatggttgat gtcagcctgc tgaaggacga gatcgcttcc
      721 gacctgatgc ggctcgtgga tttttccacg cgcaaacccg cgaagatcgc accgctcgga
      781 aaacagctgc gggcccagct cgacctgctc gccgacagca ttgcaaaaac aaaatattcg
      841 gctttcggaa cgaacgaggc caatcacatg cgtgattacc tcaacctcgt gaattccgtt
      901 tacatcaaga tattcaactt caacagcggc aaacgcgacc aggaagtgca caatctcgag
      961 aggggcggaa aaacagcttt cgtcgacctg aaaaatgatt attacaacga aggtctcggc
     1021 gacatggtga cgaacaagaa cgagttcaac aagctgctcg agcaggacgg aattctcatc
     1081 cagcgcgccg atcccgtttt cctcgaacct taccagtcgg acatcggcaa tgcgcatttc
     1141 tacgcgccca ggaaaagatt cttcggcacg tacatttcca cgttctggtt caacatcggc
     1201 gtactgtggt cgatgtcgct gctcatgttc atcacgctgt atttcgacct gctgcgcaag
     1261 atcatcgacg ggctcggttc gctcggtgaa cgtttcggga agaaaaagga ccagccgaaa
     1321 taattccaca aaaaaagcgg acgcacggtt ccgctttttt ttagccggat ttcttcagga
     1381 taattttgcg agtgcgtctt tcatccgccg cacggcttcc gtgagcttgt catcggaagt
     1441 ggcgtaagaa atgcggatgt aattgtcatc accgaacgcc gcgccgggaa caatagccac
     1501 gtgtgcctgg tcgaggagat acaggctgag atcggttgcg ttgccgatca cgtgatcgcc
     1561 gtattttttt ccgaaatacg cgctcacttc cgggaagaga tagaacgcac cgtcgggaac
     1621 attcagcttc agcccgggaa tttctttcat gcccgcgagc atgaggtcgc gcctgcgcct
     1681 gaacgcgtcg cgcatgggat gcgtggtggc gggatccata agcatcgccg cgtgcatcgc
     1741 tttctgcgtg atggaagaag tggcggaggt gaattgtccc tgcattttat cacaggcctg
     1801 tgcgatccat ttcggtgcgg cgagaattcc tccgcgccag ccggtcatgg cgaagccttt
     1861 cgaaacgccg ttgatggtga ccgtgcggtc gtaaatgaaa tcgaactgcg ccatgctttc
     1921 gaggccgccg gcgaaattga tgtgctcgta gatctcgtcg gagatgatgt agcattccgg
     1981 ctgcttcgcg atgacttccg cgatcccgcg cagctcttct ttcgaataca cgctgccggt
     2041 cgggttgcat ggagtgctga agatcatcag gcgcgtttta ggcgtgatcg ctttggcgat
     2101 ctgctccgcg gtgaccttga aatcgttttc gatcgatgac ttgataatga cgggcacgcc
     2161 ttccgccagc ttgatgatct cgaggtagct gacccaataa ggcgagggca cgatgacttc
     2221 gtcacccgga tttacgaggc tgagcacagc gtttgcgatc gcctgtttcg cgccggagga
     2281 aaccatcacc tggtcgggag aatacgccag gccgttgtcg cgcctgaatt tttcacagat
     2341 ggcttcacgc agttcgaggt agcccgaaac atgcgtgtaa tagctgaagt cgtgctcgat
     2401 cgcttcttcc gccgccttct tgatgacgtc cggcgttttg aaatcaggct cgccgagact
     2461 gaggctgatg atgtcgattc ctttggcgat cagttcgcgg ctttttcttg ccattgcaat
     2521 cgtctgcgat tcgctgagcc tgctgatccg gtctgaaaga tgcgtcatac gcgggaaatg
     2581 tgaggaggct aaaggtacgg gaagaattag gaattgatga ggaaggatta agaaataaga
     2641 atcggaggga gggttgcaaa aacttaatgc gatttgtaat tcgacaccca cgccggatcc
     2701 tgcatgtcgt gcagcaggtg gaacgcattt tcaccgtaaa tgatgtccgt gaaaaaacgt
     2761 acgccataac cctggagaat gccgatctgt ccgtcggaag gccgcagcgt gtcattcgcc
     2821 cagccggcat cggaaacgcc cgggtcctgg atatcctgct tgtgcagcgt atcggctaca
     2881 ggcgactgga tgaaaaaagg atattcgccg cagtaaagcc gccacaggtt gtcgacgggc
     2941 gagcaggcca caccgccttt gtaaagcacg tgccggatga tcgtgtccgg aaaataaccg
     3001 cagttctgca cgccgtattt cgcgaaaagg ttttcatttt tcgggttgac ggagctcgtt
     3061 ccccatcccg aagcgatggc gaggaattcg ttgcgcgtga tgggtgtgga ggaaattccc
     3121 tgcgccgggt cgtcgtatgt ccgaacggtg aaataggaaa ccggcgagct gttgggtccg
     3181 ggaataagcg catagcccag cgaatcgtgg tagacctgtg aaggttcgta cgactgctgt
     3241 aacagcccca aagcggttac ggtcagggtc aaaatgacca ggtatggatg ctttcgcaac
     3301 atacggaacc tgaatttacg aaaaataaat ggggaatgtt acgtgaaaag gcggataatg
     3361 cggaaatcct tatttttacg aggctcaaca aacacgtgac ccaagcaaac gaaacacttt
     3421 atggacgttg ccattgaact gatgaaatat atactgcctt cgctggtggt ttttgcaggc
     3481 tgttacctca tcgtcaagaa ttttctcgaa acggaaacga agcgccgcct cgcggagatc
     3541 cgcctggcga accagaacgt cgttacgccc atgcgcctcc aggcttacga acgcatcgcg
     3601 attttcctcg aacgcatcaa cccgaataca ctgatcacgc gtacgcacaa aagcggcatg
     3661 agcgcgcggt tgctccagtc ggaactcacc aaggtgatcc gcactgaatt cgagcacaat
     3721 ctttcccagc agatctacat gtcgaaccac gcctgggaaa tggtgaaaac ggcgaaggaa
     3781 gaagtgacga agctcgtcaa cgttgcggcg tcaaagctgc ccgatacggc cacaggagtt
     3841 gatctcagcc agatgatcct ccagatctcg cagcagatcg aaaagcttcc cacgcaggtg
     3901 gcgctggagt acatcaagaa agaagtggga caggtgtttt aattctgaat tcagagtgat
     3961 gagttctgag ttctgaacgt atttccatga ataagaaaac cgaggagcag cagaaaaaat
     4021 tctgtaccga ttccggtata gagataaagc gcgtttaccc gaaaaaagat atcccggaag
     4081 agcagccggg cgaattcccg ttcacacgcg gtgtgcagcc ggacatgtac cggggcaaat
     4141 tgtggacgat gcgccagtat gccggctttt caacagcgga agaatccaac aagcgctatc
     4201 attacctgct gaagaacggg accaccgggt tatctgtcgc gttcgacctt cccacgcaga
     4261 tcggttacga ttccgatcac gcgttttccg aaggagaagt ggggaaatcc ggcgtggcga
     4321 tcgattcgct ggcggatatc gagatccttt tcgacggcat taagctggaa gacgtcacca
     4381 cgtcgatgac catcaactca acggcgtcga tcctgctgag catgtacatt gcgctcgcga
     4441 aaaagcaggg cgccgatctg aaaaaaatat cagggacgat ccagaacgac ctgctgaaag
     4501 aatacgcggc ccgcggcacg tacatctatc cgccgaaggc gtccatgcgc atcatcaccg
     4561 atatttttga atactgctcg cgggaagtgc cgaagtggaa tacgatctcc atttccggtt
     4621 accacatccg cgaggcgggt tccacagcgg tgcaggagct ggcgttcacg ctcgcgaacg
     4681 ggaaggctta cctgaaagct gcgctggata aaggactcga catcgacgtg tttgcaaaac
     4741 ggctttcctt cttcttcaac gcgcacaata atttcttcga ggaagtggcc aagttccggg
     4801 cggcgcggag aatgtgggcg aagatcacaa aggagctcgg tgcgaaggat ccgcgtgcga
     4861 tgatgcttcg tttccatacg cagaccggcg gctccacgct tacggcgcag cagccgcaaa
     4921 acaacatcat ccgtgtcacg ctgcaggcgc tttcagcggt gatgggcgga acgcagtcgc
     4981 tgcacacgaa cggtttcgac gaggcgctcg cgcttcccac ggaagaggcg gcgcgtatcg
     5041 cgctgcgcac gcagcagatc attgcctggg aaagcggcgc accggaatcg gtcgatccgc
     5101 tggccggctc gtggatggtg gaagcgctga cggatgaagt ggaggcgaag gcgtgggaat
     5161 acattcggca gatcgatgca atgggcggat cggtggcggc gatcgagcag ggtttcatgc
     5221 aggacgaaat tgcggcagct tcctatgcct accaggcggc gatccagggc ggagaaaaga
     5281 tcatcgtcgg cgtgaacagg ttccaggtga aggaaaaagc aatggagaac ctgcttacga
     5341 tcgacgacag catacgcctg acgcaggtgg aaaagatcag gaaggtgaag gcgacgcgcg
     5401 acaatccgaa agtaaaaatg ctgctgggaa agatcgagtc agccgcgaaa ggaagcgaaa
     5461 atctgatgcc cgggatcctc gaagcggtgg aagcgtatgc aacgctcggt gaaatcgccg
     5521 atgtaatgcg cggggtgttc ggggaatacc ggcagtaaaa tctgaatcat gcgtttggaa
     5581 cgaccgggcg gggaagcttt tttcccgttt gatccgcaat taagaatccg gcattcaaga
     5641 ttatttggcg gagtcttcca ccaccacgaa aatatcttcc ccgtctcttt tcatcaggta
     5701 atgttcacgc gcaaaacgtt ccagctcttt gggattgctt ttcagctcgc tgatctccga
     5761 cttgttgttc gcgatctcgt tccggtaata atccgcttcc tgctgcagct tcttcacttc
     5821 ggcgcggcgg tcgacctggg aaaaaacatc gttgcggtcg aagaaaaata tccacgtggt
     5881 gaaggcaagg atggtgagca ggtatttatt actgaggacc gagactgttt tcctgaagaa
     5941 tttcatgcgc caaaggtaga ggccatccgc gctttgtgct taggtgtgga aaaatagggc
     6001 ggaatgttaa taaggcgtcc tttttccgga aagctttccg acgacgtttt ttttccggcc
     6061 aatgatcgcg aggttcggac agttctgctc ctcattttta gtataatcaa aatcaattat
     6121 ttttgtgaaa taataaatta catctatgac tttacagcag ttggaatatg cgtcggcggt
     6181 ggcgcagtgg ggcagcttcg tgacggccgc cgaaaaatgc ttcgtcacgc aacctacgct
     6241 cagcatgcag gtgaaaaagc tggaggatga agcaggcatc acgctgttcg accgcagccg
     6301 gcagcccgtt gcgctgacag acgcgggaaa attattcatc gaacaggcgc gcgtggtttt
     6361 gcgtgagcag aaaagactca aagaggtgat cgcagccacg aagggcgaca tcgcgggaac
     6421 gctgcacatc gccattatcc cgacggttgc gccgtacctt attccattgt tcgccgaaaa
     6481 gttcgccgaa aaatttcccg acgttcacat ggtcatcgag gagatgacga cggagaatat
     6541 cgtccgtgcg ctgaaggagg aagcgatcga ctgcggcatt ctcgccacgc cgctgggcga
     6601 gcattcgcta aacgaaatgc cgttgtacaa cgaacccttt gtcgcttacc tgtcgaggaa
     6661 aaatacgctg tggaataaaa agcagctgaa agccgaagat atcgacgtga cgaaagtgtg
     6721 gctgctcgcc gacgggcatt gcatgcgcac gcaggtgctg catatctgtg cggcgcgaaa
     6781 ggaaaaagcg ggcgagctga gactcgatta caaaagcggg agcatcgaaa cattgcgtcg
     6841 catgaccgat ctcggcgaag gaatgacgat cctgccctgg cttgccgttc cgggactgac
     6901 gtcaaaacag caggaagccg tgcggtattt caggtcgccg gaaccggtgc gcgagatcag
     6961 cattgtcacc caccgtgcgc agctgaaaaa agcgctgatc gacaaactgg cggaatccat
     7021 tgtcagtcat ttgccgaagc agctgaaacg ccacgcgaag caggaggttg tccgggttta
     7081 ctgacaacaa aaaaaacgaa gagcggtttc cggctcctgc aggatattcc tgatcagaac
     7141 atgattcccc agccgacctg gtacacccac catttttgag gaaccggcgc tttggcggcc
     7201 acatcctgcg gcatgaacag gccggaattg ttctgcacgt ccgtgttcac tctttggttc
     7261 atcgagttga aggaaaggaa atgccatttg ctgccgtagc ggtatttgac ggaaacgtta
     7321 taggttggac catgatccca gcggtcggtt tgtttcgacg gcaatttcac ttccatgaaa
     7381 tccgcataga tcttccctac acggagatgt ccgccgtatc ctgaaatata cgcaccggtg
     7441 gtgttcccgg tgatggacgc gccgatatcg atgtagtaat aaagcgggta ataggatacg
     7501 ccgatatcga tcaggttatt gatcctgtag caggctttga gcccggccgc gagcacatag
     7561 cttccgccta tccagggcga cgagtataaa cctcccggca gcggcaggcg ctgtttcgtc
     7621 gtgtaatcga atcttttttc cttgtttccc ccgatgatga aaccgatgat ctccgaatcg
     7681 gcgatgcgga acctgtattt gtcccgcgaa agcaaaaggt aaagcaggcc ggtgcgaagc
     7741 tctatgcctg tcccgatcac cgttttgttc acccgggcga gcgcgccgga ataggcggtg
     7801 tttccaaatg catccacgcc attgggataa gaaacgtaaa ccgtgttgga gcttcgcatc
     7861 attgtcagcg ggcaattcat ctcgtttatt ccg
//
LOCUS       DASTSF010000220        10613 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_37650,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000220 DASTSF010000000
VERSION     DASTSF010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 10613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10613
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37650"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1051)
                     /locus_tag="VFU15_06405"
     CDS             complement(<1..1051)
                     /locus_tag="VFU15_06405"
                     /inference="COORDINATES: protein motif:HMM:NF013491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HEU4717442.1"
                     /translation="MKFITEHSSAKNVTIGGKAENLFVLVREGFAVPEFIVIPKEEGL
                     SFIPEHVLRQNDFEAIKKCISDYIFPESFTASVLEEFPGAVYFSVRSSAADEDGGQHS
                     FAGQFESELFVTKETLTASIKKVWLSAYTDRVRLYRENNGIHSKHGIAVIIQRMLDPQ
                     VSGVAFGVNPASGDRNEKVISAVYGLGEGIVSGDLDADNFFVKNNSVRSQIVAKKERI
                     VFDLVNGHGTVKENVPVALQEKAALSDDEAVMIAGMLDRLRAIYKRPQDIEFAIGNKK
                     LFLLQSRPVTTTGNLPDPEGEYIVWDNSNIIESYPGLSSPLTFSFIRKMYEAVYIQFS
                     AIMGISEKEIAAHSST"
     gene            complement(1138..2097)
                     /locus_tag="VFU15_06410"
     CDS             complement(1138..2097)
                     /locus_tag="VFU15_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008511878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HEU4717443.1"
                     /translation="MTNSFSIPAENSAPFLKRFWIYQKERFPLFGHGPLVASFSFSAI
                     AYSRICRGAEGFVPWSRYAIGIVTTITLFLLVRIFDEFKDRGDDEKYRPELPVPRGLV
                     TLRELAILGACLFIVQVVLNAVFFPKMFFIYCIVIVYLCLMGKEFFVPAWLKKHQFWY
                     VTSHMFIIPLIDIYASGLDWLVEGQPAPKGLMFFFAVSYFNGIVLEIGRKIRVPGKEK
                     EGVLTYSSMLGVKKATWLWVFILAVTLSWAVAASWFAGYGKPGLVIMGGIFCVCVLPA
                     FFFLQKMTEKKSKLIEYASALWTIAMYFSLGAIPMIAEMINHS"
     gene            complement(2106..3380)
                     /locus_tag="VFU15_06415"
     CDS             complement(2106..3380)
                     /locus_tag="VFU15_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002998352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase"
                     /protein_id="HEU4717444.1"
                     /translation="MASSKEKAKHAIGLIESLKGIHALAEEFRNKTDNVAPRDNFPIE
                     EMASPEGKEKRLHFLKETIGASFGYLDGKKTITDFSSMKGNIENFIGMTQVPTGVIGP
                     LRVLGTSANGDFYVPLATSEGALVASYHRGAKATQLSGGVTSVCLVEGVQRSPVFNFV
                     DIGTAVSFLAWVYQHETKIRSIIRENSRHAELADLNANIEGNHLILTFEYHTGDASGQ
                     NMVTICTNAVCMYLVEHAPFKPVSWYIEGNYSGDKKATALAFTSVRGKKISAECVIPK
                     IIVEKVLKTTPAAMADYWQASTMGVIQSGAIGAQGHVANGLAALFLATGQDVACIAEA
                     AVGITRMQINASGDLYTSVTLPNLIVGTVGGGTALPTQKECLALIGCAGAGKARKFAE
                     ICGAVALAGEISIAAALSAGHFATAHQKLGRK"
     gene            3660..4448
                     /locus_tag="VFU15_06420"
     CDS             3660..4448
                     /locus_tag="VFU15_06420"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEU4717445.1"
                     /translation="MSACIMISETIDPAVFTKSVTAVGVSMLVGAVMIVLISVRKKKQ
                     VSPRQLWLKYFVYVLLIAGMIAGIVYDLSRYIIPVLLAGGLIEMIRVAKTAPAKPSFR
                     KVLASVLVYMIIAGFCAFMPMAGSALVLQFYTWVVIFDAFSQLFGQLLGRTKLAPVTS
                     PAKTTEGFIGGFLMLYATVLWMMPDVSWTGDFLIAFPFAVVALGGDLLASKYKRMCGV
                     KDFSSVIPGHGGILDRFDSFFFTGAVWIFLLALLALFLGGWGGD"
     gene            4568..4963
                     /locus_tag="VFU15_06425"
     CDS             4568..4963
                     /locus_tag="VFU15_06425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717446.1"
                     /translation="MKNEEENEGQEHPDFAGMFRRLNDHVETRLEYMRLLISEKIAIA
                     FAKAMSGAIQAGIFLLFFFFLNVAAACWIGKHYGDYAIGFGVVSGFYFLCWLIYMALR
                     KSVFEKKMQDSFVKALFTEQDEEDDDENE"
     gene            4956..5291
                     /locus_tag="VFU15_06430"
     CDS             4956..5291
                     /locus_tag="VFU15_06430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717447.1"
                     /translation="MSKITSMKRLRKEKQRLEQLIETQKAEIIVEAENLKNALWPVRL
                     LKRLQHTLETLAENKFVAVGAQLAYAALHAIREGKKGEEGKESDHPVVEFLKRAAQNF
                     LEMYMKKEE"
     gene            complement(5370..6347)
                     /locus_tag="VFU15_06435"
     CDS             complement(5370..6347)
                     /locus_tag="VFU15_06435"
                     /inference="COORDINATES: protein motif:HMM:NF036967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel"
                     /protein_id="HEU4717448.1"
                     /translation="MAEKLKNLFSKNTPNDLGFGTATGTGRNMNKDGTFNVKRVGEPR
                     FRPYEMYHTLITISWTKFIFLVLCFYLSVNLVFAFIYYFIGIDHLTSMDHPMTETRKF
                     IEGFFFSSQTLTTLGYGRVAPIGILASSVAAVESMMGLLGFALATGLLYGRFSRPQSK
                     ILYSRHAVVAPYKGATGFMFRTSNLRRNQLIEVEVEVSIGFTEPGSRSRNFLQLDLER
                     KRINLFPTSWTIVHPIDENSPFYGRTLEDVKGMNPEIFVILKAFDDTFSQTIYSRTSY
                     RADEIIYGAKFLPMFHQLENGITQLDLGLIDAVEKVELPQMEMNQLETT"
     gene            complement(6373..7806)
                     /locus_tag="VFU15_06440"
     CDS             complement(6373..7806)
                     /locus_tag="VFU15_06440"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEU4717449.1"
                     /translation="MNFFRKKLPVITVSLLSLALVIFKWKDLSLPAYWDEGFPYVYAI
                     RYLADHHLTLLPSGMPPLYSTGHPTLFYFLAAAWMKISGTGIATAHIFPLAISVVLLF
                     AVYRFGKIFFSEWAGVFAVLLLATRAVFLAQSSMLLPENLVALFMLLAFEAWLKKRNW
                     LYFTWASLLLLTKEPGLVFIGVIGLYDFFTLLRTREIPFGKKIRILAVIASPVLPAVL
                     FFIVQRIQLGWFFFPRHIHSFETTPGEYYDKLVKGFGSQFFVYYGGLFITIAIASLLA
                     YGRKQLKYITAVQRTFLRTVPLIVVAYILFFSFSFPIPRYILCLYPLWFLMAGFLATQ
                     LLQPVKKFAAPALLFLLAAIQAGYAYDIRSQADFDLGYADGVRVEQQAIDWCVKNNYM
                     NSPVFAQEILQRGMTSDADGFVSPENIFHNVGHSFNDSTRLFIFTSFLPEPNETEARK
                     LPLELLQRFTSGKAWIEIWTWKTGDSL"
     gene            7921..10395
                     /locus_tag="VFU15_06445"
     CDS             7921..10395
                     /locus_tag="VFU15_06445"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEU4717450.1"
                     /translation="MKFFYRLCLVPALAFSGQLFAQVPCGAVFYDGFESGSYAPAWTV
                     GSASTVWSVTATNPASGTYRLEGTGADNTHMTGLSTTFASCTPSTISWDMYPNGTGAC
                     NYVVGGDNAVTPTNCVFFCYYTSSTNGIRFVSGSTVQYTVPVINTWYHVEMRNINWSA
                     HTFDIYIDNNLITTGFAFRSSSENSLTNLNLYNYTSGTFGAWDNINVGGTFITANSFS
                     SSVSCNGGSDGGVSLNVSGGTPAYSYSWSTGATTQNVSGLTAGTYSVVITDAGGCHDT
                     LSSISVTEPTPVMSTASVSDVQCNGGGNGGIDQAPGGGTPGYTFMWSDGSITEDIAAL
                     AAGTYSVTVTDMNGCTGTATYVVTEPPAITTSVSSTGVLCNGGSDGSASITPSGGTPG
                     YTYAWNTGDVTQNISNEPAGTYSCSVTDANGCQATGLVTITEPAALSMMLSFSQVTCN
                     GLSDGSVTANVSGGTPSYTYNWSNGGTAQSISSLSPGTYVCTLSDMNGCSGLDSVTIT
                     EPSALALALSSLDVTCPGGTNGAASVAVAGGTPGYTMMWSNSATTDTTMNLSAGVYSV
                     NVTDMNGCTAADSVSISAPAPFSLTAATSDPTTCAGNDGTIDLTVSGGTPGYSFSWSN
                     SAATEDLYFLSAGTYSVDVTDTNGCTSGASFLLNDPAPPVVTLSLPGATHCVNDAPFA
                     LTGGSPAGGTWSGTGVSGSNFNPSVSGTGSFVVTYTYTAPTGCSASATDTVTVSSCTG
                     IDEMPADQLISVYPNPTNGNVALNCNNISGEITAEIINPLGQVIEARTFTGNRLDFDL
                     SAQPAGVYLVRVRTSQGTVAKKVVKQ"
BASE COUNT         2506 a         2731 c         2893 g         2483 t
ORIGIN      
        1 acgtggaaga atgcgccgcg atctcttttt ctgaaattcc cataatggcg gagaactgga
       61 tgtacaccgc ttcgtacatt ttccggatga acgaaaaggt gaggggagag gaaagaccgg
      121 ggtacgattc gatgatgttg ctgttgtccc acacgatgta ttcaccttcc gggtcgggaa
      181 gattcccggt cgtcgtaacg ggccggcttt gaaggagaaa aagttttttg ttcccgatcg
      241 caaactcgat gtcctgcggg cgtttgtaga tcgctctcag ccggtcgagc attcccgcga
      301 tcatcaccgc ttcgtcatcc gaaagcgctg ctttctcctg cagcgcaacg ggtacatttt
      361 cttttactgt tccatgaccg ttgacaaggt cgaaaacgat gcgctctttt ttcgccacga
      421 tctgcgaacg gacggaattg ttcttcacga aaaaattatc cgcgtcgaga tcgccggaca
      481 caattccttc tcccagcccg taaaccgcgc tgatcacttt ttcgttgcgg tcgccgctgg
      541 ccgggttgac gccgaaggcc acgccggaaa cctgcgggtc cagcattcgc tgtatgatca
      601 ccgcgatgcc gtgcttcgag tggatgccgt tgttctcacg gtaaagacgc acgcgatcgg
      661 tgtacgccga aagccatact tttttaatgg aagcggtaag ggtttctttc gttacaaaaa
      721 gttccgactc gaactgtccc gcaaaagaat gttgtccgcc gtcttcgtcg gctgcggaag
      781 aacggacgga aaaataaacc gctcccggga actcttccag cacggaagca gtaaagcttt
      841 ccgggaaaat gtaatccgag atgcattttt ttatcgcttc gaaatcgttt tgccggagca
      901 catgttcggg gatgaaggac aaaccttctt cctttggaat gacaatgaat tccggcacgg
      961 cgaaaccttc acggacgagc acgaaaagat tttcggcctt accgccgatg gttacgtttt
     1021 tcgctgaact atgttctgta ataaatttca taacgttctt ttccccgtgt gcatcaccgg
     1081 tgaaccggtt tcagttgccc gggcgttccg ccggatcttc aaatcctccg gtattaatta
     1141 tgaatgattg atcatttccg ctatcatggg tatcgcaccc agtgaaaaat acatcgcgat
     1201 cgtccagagc gcggaagcat attcgatgag ctttgatttt ttctccgtca tcttctgcaa
     1261 gaagaaaaac gcgggaagaa cgcacacgca gaaaataccg cccattataa cgaggcctgg
     1321 ttttccgtaa ccggcgaacc acgatgccgc aaccgcccag gaaagcgtta cggcgaggat
     1381 gaatacccag agccatgtcg cttttttcac accgagcatg gacgaatagg tgagcacgcc
     1441 ttctttttct tttcccggta cccgtatctt ccgcccaatc tccagcacaa tgccgttgaa
     1501 atacgaaacc gcgaaaaaga acatcagtcc tttcggggca ggctgccctt ccacgagcca
     1561 gtcgaggccg ctcgcgtaaa tgtcgatgag cggaataatg aacatgtgcg acgtcacgta
     1621 ccagaactgg tgctttttca gccaggcggg aacgaaaaat tcttttccca tgaggcagag
     1681 gtagacgatg acgatgcagt agatgaagaa cattttcggg aagaagaccg cgttcagcac
     1741 aacctgcaca atgaaaagac atgcgccgag gatcgccagc tcgcgcagcg taacgagtcc
     1801 gcgcggaacg ggaagctccg gcctgtattt ttcatcgtcc ccgcggtctt tgaactcgtc
     1861 gaaaatgcgc acgagcagga aaagcgtgat cgtcgtgacg atgccgatcg cgtaacggct
     1921 ccagggaacg aatccttccg caccccggca gatgcgggaa taggcgatcg cagaaaaact
     1981 gaacgaagcc accagcggtc cgtgcccgaa cagcgggaaa cgttccttct ggtagatcca
     2041 gaagcgtttc aggaaaggcg cggaattttc cgcgggtatg gaaaatgagt tggtcatcgg
     2101 aaaaattatt ttcgtccgag cttctggtgc gcagtggcga aatgtcctgc ggataatgca
     2161 gcggcgatgg aaatttcacc ggccagcgca acggccccgc aaatctccgc gaacttccgt
     2221 gctttgcccg cgcccgcgca gccgatgagc gcgaggcatt ctttctgcgt gggaagcgct
     2281 gttccgccgc ccacggtccc gacgatgagg ttcggcagcg tgacgctcgt gtaaagatcg
     2341 ccgcttgcgt tgatctgcat gcgcgtgatt cccactgcag cttccgcgat gcacgcgacg
     2401 tcctgtcccg tggcgaggaa caaagcggcc agcccgttcg cgacatgtcc ctgcgcgccg
     2461 atcgcgccgc tctggatgac gcccatcgta gatgcctgcc agtaatccgc catggcggcg
     2521 ggtgttgtct tcagcacttt ttccacgatg atcttcggga tcacgcattc cgcggaaatt
     2581 tttttgccgc gcacagatgt aaaggcgagc gctgttgctt ttttgtcgcc ggaataattt
     2641 ccttcgatgt accacgaaac cggtttgaac ggagcgtgct ccacgaggta catgcatacg
     2701 gcgttcgtgc agatcgtcac catgttctgc ccggaagcgt cgccggtatg gtattcgaag
     2761 gtgaggataa ggtggttccc ttcgatgttc gcgttcaggt cggcgagctc tgcgtgcctg
     2821 ctgttctcgc ggatgatgga gcggatcttc gtttcatgct ggtagaccca cgcaagaaaa
     2881 gaaaccgcgg tgccgatatc gacgaaattg aaaacaggac tgcgctgcac accttccacg
     2941 agacagacgg aagtgactcc gccgctcagc tgcgtggctt ttgcgccgcg gtgataggaa
     3001 gcgacaagcg ccccttcact cgtggcgagc ggaacgtaaa aatccccgtt cgctgaagtg
     3061 ccgagcacgc gcagcggacc gatcacgccg gtgggtacct gcgtcatccc gatgaaattt
     3121 tcgatgttcc ctttcatcga tgaaaagtcg gtgatggttt tttttccgtc gaggtaaccg
     3181 aacgaagcgc cgattgtctc cttcagaaaa tggaggcgct tctcttttcc ttccggcgaa
     3241 gccatttctt cgatagggaa attatcgcgc ggcgcgacgt tgtccgtttt gttgcggaac
     3301 tcttccgcca gcgcatgaat tcctttcagc gattcgatca ggccgatcgc gtgttttgct
     3361 ttttctttcg aagatgccat ggtgatgatt tttcgtttga cagggcaaat ctatgaccgc
     3421 aggagtgccc ccgaaaccgg aacgtgcaaa ggaatgggaa atcttttgcg gaattccggg
     3481 aaataagagc ggcacgcatc aattctaagg agtgatttgc gattttcgca acgcggcgtt
     3541 cccgattggt gttatttgcc tgaattgttt tgaaagcacg ccttttattt tgaatttggg
     3601 gaacccaaaa agcgttaaaa aacttacggg aagatgtttt cccctatggc cgaaaacgga
     3661 tttccgcctg tattatgatc agcgagacga ttgatccggc tgttttcaca aaatcggtca
     3721 cagcggtggg cgtaagcatg ctcgtcggcg ccgtgatgat tgtcctgatc tccgtgcgca
     3781 aaaagaaaca ggtttctcca cgccagctct ggctgaagta ttttgtttac gtgctgctga
     3841 tcgcgggaat gatcgcagga attgtttatg atctttcgcg ctacatcatt cctgttttgc
     3901 tggccggcgg actgatcgag atgatccgcg tggcgaaaac cgcaccggca aaacccagct
     3961 ttcggaaggt gcttgcatcc gttctcgttt acatgatcat cgccggtttc tgcgcattca
     4021 tgccgatggc aggaagcgcg ctggtgctgc agttttacac ctgggtggtg atcttcgatg
     4081 cgttttccca gcttttcggg caattgctcg gaaggacaaa gctcgcgccc gtgacgagtc
     4141 cggcaaaaac cacggaaggt tttatcggcg ggttcctcat gctgtatgcc actgttctgt
     4201 ggatgatgcc ggacgtgagc tggacaggcg attttctcat tgctttcccg ttcgcggtgg
     4261 tggcgctcgg cggagacctg ctggcgtcga aatacaaacg tatgtgcggc gtgaaggatt
     4321 tcagtagcgt cattcccggt cacggcggaa ttctcgaccg tttcgacagc tttttcttca
     4381 ccggcgccgt ctggatattt ttgctggcgc ttttggcctt gttcctcggc gggtggggcg
     4441 gagattagga attccacggc tgattacttc cctgtgcgga actgagtttc atcatgtcat
     4501 cccggtgcat gttcacgatt tttgttattc gtaaaaagag cttcagtaat tgttttaaat
     4561 tgatcacatg aaaaacgaag aagagaacga aggacaggaa catcctgatt ttgcaggaat
     4621 gttcaggcgc cttaacgacc atgtcgaaac gcggctggaa tacatgcgcc tgctgatctc
     4681 cgaaaagatc gcgatcgcat ttgcaaaagc tatgtcgggc gcgatacagg cgggaatttt
     4741 cctgctgttt ttctttttcc tcaacgtggc ggcggcctgc tggatcggga agcactatgg
     4801 cgattatgcg atcggtttcg gcgttgtatc cggtttttat tttttgtgct ggctgatcta
     4861 tatggcgctt cgcaaatctg tgtttgaaaa gaaaatgcag gacagcttcg taaaggcatt
     4921 attcaccgaa caagacgagg aggacgacga tgaaaatgag taagatcacc agcatgaaac
     4981 ggctgcggaa agagaagcag cggttggaac aactgatcga aacgcagaaa gcggagatca
     5041 ttgtggaagc agaaaacctg aagaatgcgc tctggcccgt gcggctcctg aaacggctgc
     5101 agcatacgct cgagacgctg gccgaaaaca agttcgtagc cgtcggtgcg cagctggctt
     5161 acgccgcgct tcatgcaata cgcgaaggca aaaaaggcga agaaggaaag gaaagcgatc
     5221 atcccgtggt ggaattcctg aagcgggccg cacagaattt cctggagatg tacatgaaaa
     5281 aggaggagtg ataattgcca gtgagtaatt gaggatgaat aatgaccagg tgcccgtaac
     5341 ctggaatttt atttttggat ttttctgcgt caggttgttt ccagctggtt catttccatc
     5401 tgcggtaatt ccactttttc cacggcatcg atcagtccga ggtcgagctg cgtgatcccg
     5461 ttttccagct ggtggaacat gggaaggaat ttggcgccgt aaatgatttc atcggcgcgg
     5521 tacgatgtgc gcgaatagat cgtctgggaa aacgtatcgt cgaacgcttt gaggatgacg
     5581 aaaatttcgg ggttcatgcc tttcacatct tccagtgtgc ggccgtagaa cgggctgttc
     5641 tcgtcgatcg ggtgaacgat ggtccagctg gtgggaaaaa gattgatgcg cttgcgttcg
     5701 agatcgagct ggaggaaatt ccgcgaacgc gaacccggtt cggtgaaccc gatggaaact
     5761 tccacttcca cttcgatgag ctggttcctg cgcaggttcg acgtgcggaa catgaaccct
     5821 gtcgcccctt tgtacggcgc aacgaccgcg tgccggctgt agagtatttt cgactgcggg
     5881 cgtgaaaagc gtccgtaaag caggcccgtg gcgagcgcaa agccgagcag tcccatcatc
     5941 gattccaccg cggcgacgct gctggcgaga atgccgatgg gtgcaacgcg cccgtagccg
     6001 agcgtggtga gcgtctgcga gctgaagaaa aatccttcga tgaatttccg tgtttccgtc
     6061 atcgggtgat ccatcgacgt aaggtgatcg atcccgatga aatagtagat aaaagcgaag
     6121 acaaggttga ccgaaagata gaagcacagc acgaggaaaa tgaacttcgt ccacgagatc
     6181 gtgatgagcg tgtggtacat ttcgtacgga cggaacctgg gttcgcccac gcgtttcaca
     6241 ttgaacgtgc cgtccttgtt catgttgcgg cccgtgccgg tggcggtccc gaaaccaagg
     6301 tcgttcggtg tgttcttcga aaaaagattt ttgagctttt cggccatgga acaaaattaa
     6361 aggatcagcg cgtcataagc tatcgccggt cttccacgtc catatttcga tccaggcttt
     6421 gccggaggtg aatctttgca gcagctccag gggcagcttt ctcgcttccg tttcgttcgg
     6481 ttcgggaaga aaagaagtga aaatgaacag gcgcgtactg tcgttgaacg aatgccccac
     6541 gttatgaaaa atgttttccg gcgaaacgaa tccgtccgca tccgaggtca tgccccgttg
     6601 caggatctcc tgcgcgaaaa caggcgagtt catgtagttg ttctttacgc accagtcgat
     6661 cgcctgctgt tccacgcgaa caccgtccgc atagccaaga tcgaaatcgg cctgcgaacg
     6721 gatgtcgtac gcatagccgg cttgtatggc ggccagcagg aacagcaatg caggtgctgc
     6781 gaattttttc acgggctgca gcagttgtgt cgctaaaaaa ccggccatga gaaaccataa
     6841 aggatagaga caaagtatgt agcgcggaat gggaaagctg aagctgaaaa aaagaatata
     6901 tgccacaacg atcagcggaa ccgtacggag aaatgtccgt tgcacggctg tgatatattt
     6961 cagttgcttc cttccgtatg caagcagcga cgctatagcg atcgtgatga acaatccgcc
     7021 gtaatagacg aagaactgcg acccgaaacc tttaacgagc ttatcatagt attcgccggg
     7081 agtcgtttcg aacgaatgga tgtgtcgcgg aaaaaagaac cagcccagct ggatgcgttg
     7141 cacaatgaaa aataaaacgg cgggaagaac cggtgatgcg atgacagcca gtattcttat
     7201 tttttttccg aagggaattt cacgcgtgcg cagcaaagtg aaaaagtcgt acagcccgat
     7261 cacgccgatg aagacgagac ccggctcttt ggtcagcagc agcagcgaag cccacgtaaa
     7321 ataaagccag ttccttttct tcagccatgc ttcaaatgcg agaagcatga aaagcgccac
     7381 aaggttttca ggcagcagca tggacgactg cgcgaggaaa accgcgcgcg tggcaagcag
     7441 cagcacagcg aagacgccgg cccattccga aaagaaaatt tttccgaagc ggtaaacggc
     7501 aaagaggaga acaacggaaa tcgccaatgg aaaaatatgc gctgttgcga tcccggttcc
     7561 tgaaattttc atccacgcag ccgcaaggaa atagaacaat gtcgggtgac ctgttgaata
     7621 aagcggaggc atgcccgatg gcaaaagcgt taagtgatga tcggcaaggt agcggatcgc
     7681 atacacgtac gggaagcctt cgtcccagta ggccggcagc gaaagatcct tccacttgaa
     7741 aatcacaagt gccagcgaga gcagcgaaac ggtgatgacg ggaagttttt tccggaagaa
     7801 attcacgtga tgaaattacg tatttgacaa tcctttttcg cagagaatat atttctgaat
     7861 ttaacccgga tgagtaaatt tggccaaatt gaatcgtttt aatcccataa atcaaaacac
     7921 atgaaatttt tttacaggtt gtgtctcgtt cccgctctcg cattttcggg acaactcttc
     7981 gcgcaggttc cctgcggagc cgtcttctat gatggtttcg aatcgggttc ctatgccccg
     8041 gcgtggactg taggttctgc atccaccgta tggagcgtca ctgccacaaa tcccgcgtcg
     8101 ggaacgtacc ggctggaagg taccggcgct gacaacacgc acatgacggg tctcagcacg
     8161 acattcgcca gctgcactcc atcgaccatc agctgggaca tgtatccgaa cggcaccggt
     8221 gcatgcaatt acgttgtagg cggggacaat gccgttaccc ccaccaattg tgtattcttc
     8281 tgctattata caagctccac gaatgggatc cgatttgttt ccggctctac tgtacaatac
     8341 accgtcccgg tgataaatac ctggtaccac gttgaaatgc gcaacatcaa ctggtcggcg
     8401 catacgtttg atatttatat cgacaacaac cttataacaa ccgggtttgc ctttcgttca
     8461 tcctcggaaa attcgcttac caacctgaat ctttacaact acacttccgg cactttcggg
     8521 gcgtgggata atatcaacgt cggtggcacg ttcattaccg cgaacagctt ttcttcttcc
     8581 gtttcctgta atggcggaag cgacgggggc gtcagcctga atgtttcagg cggaactcct
     8641 gcttacagct attcctggag cacgggcgcg accacgcaga atgtttccgg tcttactgca
     8701 ggaacgtaca gtgtcgtgat cactgatgca ggtggttgcc atgatacgct cagcagcatt
     8761 tctgtaacag aacccacgcc ggtgatgagc accgcatccg tcagcgatgt gcaatgcaac
     8821 ggcggtggta acggcgggat cgaccaggcg ccgggcggag gaacgccagg atacacattc
     8881 atgtggagcg atggttccat tacggaagac attgccgcgc tcgcggcggg aacctattcg
     8941 gtcactgtta cggatatgaa cggatgcacc ggcaccgcca cgtacgtggt tacggaaccc
     9001 ccggcgatca ccacttccgt ttcttccacc ggtgtgctgt gcaacggtgg cagcgacggt
     9061 tctgcttcca tcactccttc gggcggaaca cccggttaca cgtatgcgtg gaataccggc
     9121 gatgtgacgc agaacatttc gaacgaacct gccggcacct attcctgttc tgtcaccgat
     9181 gcgaacggtt gccaggcaac cggcctggta acaatcaccg aaccggctgc actatccatg
     9241 atgctttcat tctcgcaggt aacctgcaac ggtttatccg acggttcggt aaccgctaac
     9301 gtttccggcg gaactccttc gtacacgtac aactggagca acgggggaac ggcgcaaagc
     9361 atttcttccc tttcaccggg aacgtacgtc tgcacacttt ccgatatgaa cggatgttcg
     9421 ggattggatt ccgtcacgat cacggaacct tcggcgcttg cacttgcgct ttcttctttg
     9481 gatgttactt gtccgggcgg aacgaacggg gctgcttcgg tcgcagtcgc gggaggaaca
     9541 cccggctata caatgatgtg gagcaattcc gccacgaccg acaccacgat gaatctttca
     9601 gcaggagttt attcggtgaa tgtgaccgac atgaacggat gcacggccgc tgacagcgtg
     9661 agtatttccg caccggcccc gttttcgctg acagcagcaa catccgatcc gacaacctgc
     9721 gcggggaacg acggcacgat tgatctcacc gtcagtggcg gaacaccggg ctacagtttc
     9781 tcgtggagca attccgcggc gacggaagat ctttatttcc tctcggccgg aacctattcg
     9841 gttgacgtga ccgacacgaa cggttgcacc agcggcgctt ccttcctgct caacgatcct
     9901 gctccgccgg ttgtcacgtt atcgcttccc ggcgcgacgc attgcgtgaa cgatgcgcca
     9961 ttcgcgctca cgggcggttc gcctgcaggc ggaacgtgga gcggaacggg tgtgagcggt
    10021 tcgaatttca atccttcggt ttccggcacc ggttcatttg tcgtgacgta tacatacaca
    10081 gcgcccaccg gctgttctgc atcggcgaca gataccgtta cggtttcttc ctgtacggga
    10141 attgatgaaa tgccggcgga ccagctgatc tctgtatatc ctaacccgac caacggcaac
    10201 gtggcgctga attgcaataa catcagcggg gagatcacgg cggaaatcat caacccgctc
    10261 ggacaggtga tcgaggcgcg cacattcacc ggcaatcgcc tcgacttcga cctcagcgca
    10321 caaccggcag gcgtttatct cgttcgcgtg cgcacttcgc agggaacggt tgcgaagaaa
    10381 gtggtgaagc agtaaattct caataaaatt taaccggaaa aggatcgcga aagcgatcct
    10441 ttttttataa caggtacgga aacgtatttc accacgcccc tccgatgctg aggccgaacc
    10501 agaggggata aaaaagtctg tcattcccga attttttaga aaaacaactg ggcagtacct
    10561 tgggagtaaa gctgacccgg aaaagaagat gatctcttac gtctcgaaac tgg
//
LOCUS       DASTSF010000221         2871 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1760152,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000221 DASTSF010000000
VERSION     DASTSF010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2871
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1760152"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..392
                     /locus_tag="VFU15_06450"
     CDS             <1..392
                     /locus_tag="VFU15_06450"
                     /inference="COORDINATES: protein motif:HMM:NF012549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEU4717451.1"
                     /translation="VYDILKFLKEDNEMNYHFLTTMCGLHYPDHKGKELGVMYQLHNM
                     PKNNRIRLKTFFPIADPEMPTVTSLWATANWMERQEYDFFGIIFKGHPNLKRILNMED
                     ITFFPMRKDFPLEDPTRDDKDDSMFGR"
     gene            415..1626
                     /locus_tag="VFU15_06455"
     CDS             415..1626
                     /locus_tag="VFU15_06455"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HEU4717452.1"
                     /translation="MSETTEPTAPATEEKQYSTLNLGPTHPATHGIFQNVLKMDGEII
                     VDSEPTIGYIHRAFEKIAERRPFYQVTTLTDRMNYCSSPINNMGWHMTVEKLIGVKLP
                     KRVEYMRVVAMELSRIADHIICNSVIGVDTGAMTGFLYIFQWREYIYEIFEEICGARL
                     TTNMGRIGGFERQWSPKVWEKIDAFVRDFPDALKEFENLLVRNRIFMDRTINAGPISG
                     DRALEYGFTGPNLRAAGVDYDVRVMNPYSSYEDFEFSIPVGTQGDVYDRFMVREQEMW
                     ESLSIIKQALAKIDRNENVFHADVPDFYLPPKEEVYNNMEALIYHFKIVMGEDEIPVG
                     EVYHSVEGGNGELGFYLISDGGRAAYRLHLRRPCFIYYQAYPEMIKGGMLSDAILTMS
                     SMNVIAGELDA"
     gene            1654..2193
                     /locus_tag="VFU15_06460"
     CDS             1654..2193
                     /locus_tag="VFU15_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007844971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HEU4717453.1"
                     /translation="MAKEIKFTDETMAVVQRILKRYPEGKQKSAIIPLLHLAQMEFDG
                     WLDAPVMDYVASILKIQPIEVYEVASFYSMFNLKPVGKCLIEVCRTGPCWLLGAEDIV
                     RHIEKKLNIKDGETTSDGMFTLKTVECLAACGTAPVIQVGETYHENMTCEKTDEMLNE
                     YRSKGERKTHWDKKHIKAQ"
     gene            2216..>2871
                     /locus_tag="VFU15_06465"
     CDS             2216..>2871
                     /locus_tag="VFU15_06465"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003000201.1"
                     /note="part of NADH-ubiquinone oxidoreductase complex I;
                     shuttles electrons from NADH, via FMN and iron-sulfur
                     (Fe-S) centers, to quinones in the respiratory chain; NuoF
                     is part of the soluble NADH dehydrogenase fragment, which
                     represents the electron input part of NADH dehydrogenase;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit F"
                     /protein_id="HEU4717454.1"
                     /translation="MGKQLLLENINVPGIDTLAVYRSHGGYSALEKALKKKPEELVDE
                     VKKSGLRGRGGAGFPTGMKWGFIAKPEGVPRHLVCNADESEPGTFKDRFLMQHKPHIL
                     IEGMIISSYALGANTSYIYIRGELLYVYHILEKAIAEAYEAGFLGKNILGSGFDLDLY
                     VHPGGGAYICGEETALIESLEGKRGNPRIKPPFPAVAGLFAQPTTINNVETLAAVPHI
                     L"
BASE COUNT          743 a          791 c          749 g          588 t
ORIGIN      
        1 acgtgtacga tatcctgaaa ttcctgaagg aagacaatga aatgaattat catttcctca
       61 ccacgatgtg cggactgcat taccccgacc acaaaggcaa agagctgggc gttatgtacc
      121 agctgcacaa catgccgaag aacaaccgca tccgcctgaa aacattcttc cccattgcag
      181 atcccgaaat gccaacggtg acttctctct gggcgacagc caactggatg gaacgccagg
      241 aatacgattt tttcgggatc attttcaaag gtcatcccaa cctgaagcgc atcctgaata
      301 tggaagacat cacgttcttc cccatgcgca aggatttccc gctggaagat cccacacgcg
      361 acgacaagga cgattcaatg ttcggacggt aaaaagataa gcgcgattac tattatgagc
      421 gaaacaacag aaccaacagc tcccgccacc gaagaaaagc agtattccac gctcaacctc
      481 ggtcccacgc acccggccac gcatggaatt ttccagaacg tgctcaagat ggacggagaa
      541 atcatcgtgg attcggaacc caccatcggg tacattcacc gcgcatttga aaaaattgcg
      601 gagcgccgcc cgttctacca ggtcaccacg ctgaccgacc gcatgaatta ctgctcatcg
      661 cccatcaaca acatgggctg gcacatgacg gtggaaaagc tcatcggcgt gaagctgccc
      721 aagcgcgttg aatacatgcg cgtggtggcg atggaacttt cccgcatcgc ggatcacatc
      781 atctgcaatt cggtgatcgg cgtcgatacg ggcgcgatga ccggcttcct ttacatcttc
      841 cagtggcgcg aatacattta cgagatcttc gaagagatct gcggcgcacg cctcaccacg
      901 aacatgggac gcatcggcgg gttcgaacgc cagtggtcgc cgaaagtgtg ggaaaagatc
      961 gacgcattcg tccgcgattt tcccgatgcg ctgaaagaat tcgaaaacct gctcgtgcgc
     1021 aaccgcatct tcatggaccg cacgatcaat gccggaccta tctccggcga ccgtgcgctc
     1081 gaatatggtt tcactggccc caacctgcgc gcagccggtg ttgattacga cgtccgtgta
     1141 atgaacccgt attcttccta cgaagatttt gaattctcca ttcccgtcgg cacgcagggc
     1201 gatgtttatg accgcttcat ggtacgcgag caggaaatgt gggaatcgct gagcattatc
     1261 aaacaggcgc tggcgaagat cgaccgcaac gaaaatgttt tccatgcgga tgttccggat
     1321 ttttaccttc cgccgaaaga ggaagtgtac aacaacatgg aggcgctcat ctatcacttc
     1381 aagatcgtaa tgggtgaaga tgaaattccg gtcggtgaag tttatcacag cgtggaaggc
     1441 ggcaacgggg agcttggttt ctacctcatc agcgatggcg gacgcgcggc ttaccgcctg
     1501 cacctgcgcc gtccctgctt tatctattac caggcttatc ccgaaatgat caaaggaggc
     1561 atgttgtcgg acgcgatcct gaccatgtcg agcatgaatg tgatcgcagg agagcttgat
     1621 gcataaaaag aacccgtgtt aatccgttaa tccgtggcta aagaaataaa attcactgac
     1681 gagacgatgg ctgttgtgca gcgcatcctc aaacgttatc cggaaggaaa gcagaaatcc
     1741 gcgatcatcc cgctgctgca cctggcgcag atggaattcg acggctggct cgatgctccg
     1801 gtgatggatt acgtagcgtc gatcctgaaa atacagccga tcgaagtgta cgaagtcgct
     1861 tcgttctact cgatgttcaa tcttaagccc gtggggaaat gcctgatcga agtctgccgc
     1921 acaggcccct gctggctgct cggtgcggag gatatcgtgc gccacatcga gaaaaagctg
     1981 aacatcaaag acggggaaac cacttccgac ggaatgttca cgctgaaaac cgtggaatgc
     2041 ctcgcggcct gcggaaccgc tcctgtcatc caggtaggag aaacctatca cgagaacatg
     2101 acctgcgaga aaacggatga gatgctcaac gagtaccgct ccaaaggcga acgtaaaacg
     2161 cattgggata aaaaacacat taaagctcag taagtaataa ccgcgcttat caattatggg
     2221 aaaacagctg ctgcttgaaa atatcaacgt tccggggatc gacaccctgg cggtttaccg
     2281 ttcacatggt ggttattccg cgctggaaaa agcgctgaag aaaaaaccgg aagagcttgt
     2341 cgatgaagtg aaaaaatccg gcctgcgcgg tcgcggcggc gcgggatttc cgacaggaat
     2401 gaagtggggc ttcatcgcaa agccggaagg tgttccgcgt cacctcgtct gcaacgccga
     2461 cgaatcggag ccgggcacgt tcaaggaccg tttcctgatg cagcacaagc cgcacattct
     2521 catcgaggga atgatcattt ccagctatgc gctcggcgcg aacacttctt acatctacat
     2581 ccgcggcgag ctgctgtacg tttatcacat cctcgaaaaa gcgatcgcgg aagcctacga
     2641 agccggtttc ctcggtaaaa atatcctcgg ctcggggttc gatctcgatc tttacgttca
     2701 tcccggcgga ggcgcgtaca tctgcggaga agaaaccgcg ctgatcgaat cgctcgaagg
     2761 aaaacgcggc aacccgcgca tcaagccccc gttccccgcg gtggccggcc tgttcgcgca
     2821 gcccacgacg atcaacaacg tcgagacgct cgccgcggtg ccgcacatcc t
//
LOCUS       DASTSF010000222         2483 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1163214,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000222 DASTSF010000000
VERSION     DASTSF010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2483
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1163214"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(157..837)
                     /locus_tag="VFU15_06470"
     CDS             complement(157..837)
                     /locus_tag="VFU15_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU4717455.1"
                     /translation="MKILLVEDEPGLRNSISQFLEKEGNVIETAEDFLRGSEKADIYD
                     YDCVVVDITLPGGNGLDIIRQLKKAKSKAGIVIISAKNSLDDKITGLDIGADDYLPKP
                     FHLPELNARIKSVVRRISFGGENEILHGCIRVFPAKKKVTVHSKEIALTKKEYDLLLF
                     LIGNRDRILTKEAIAEHLWGDSVDTADSFDFIYTHIKNLRKKIVDAGGEDYLRTVYGT
                     GYKFAGEA"
     gene            complement(877..1416)
                     /locus_tag="VFU15_06475"
     CDS             complement(877..1416)
                     /locus_tag="VFU15_06475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717456.1"
                     /translation="MRFLLLLLLLSGTAAVHNSPDLGDIRKKLFFNAPFDSTVTPRQL
                     AAFFDSKPDSFYTTKIVADSSDTTEIRYYYEYRTKFTDPVFHCPMRIEFAGSRRRGEK
                     VRLEKLRVAPAEWVRSSWEANRLYRKVNRIACMPSMHSHRGFHGQEYWKTKEILLGNV
                     LFVNGKEESNGNIWGLFLR"
     gene            complement(1454..2092)
                     /locus_tag="VFU15_06480"
     CDS             complement(1454..2092)
                     /locus_tag="VFU15_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HEU4717457.1"
                     /translation="MEIEIWSDVMCPFCYIGKRHFEKALEQFAEKDSVRVTWKSYQLD
                     PALKPEGEQNIYDYLAERKGISRQQSETMHERVVRMAEAAGLHYDFDKAIVANSFDAH
                     RLSHLAAKHGLQDAMEERLFAAYFTEGKNIGDPETLVQLGAEVGLDENEIRGMLRSDA
                     FSAGVKQDADEAAQLGARGVPFFVIDRKYGISGAQPVETFLRALDEAAKTGR"
     gene            complement(2103..>2483)
                     /locus_tag="VFU15_06485"
     CDS             complement(2103..>2483)
                     /locus_tag="VFU15_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014773259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dioxygenase"
                     /protein_id="HEU4717458.1"
                     /translation="YYQSARFHFDLARQLKPLREKGVLIIGSGAVVHNLRAAAPKMFG
                     GDHTLYGWEEEFDSWVKKKLDEGDIRSLIDYEKSPLGLMAAPTPDHYVPMLYSLGVME
                     KDEQIEHTFEELLPAFSNRGFLIH"
BASE COUNT          520 a          671 c          646 g          646 t
ORIGIN      
        1 cgacataatc ttcgatgtgt tttttttcaa tgaagatgcc ctcggtcact tcctcgtcga
       61 ggatgctgcg gaggttgaaa tagaacacca cgccgccgat gacgaagacg agcagcgtgc
      121 tgacgaggta aatgatcgat gtgcgtgtca gcagcttcat gcttcacctg cgaatttgta
      181 tcctgttccg taaaccgtgc gcaggtaatc ttcaccgccc gcatcgacga tctttttccg
      241 taggttcttg atgtgcgtgt aaataaaatc gaacgaatcc gccgtgtcga cgctgtcgcc
      301 ccacaggtgc tcggcgatgg cttccttcgt gaggatgcgg tccctgttgc cgatgaggaa
      361 gagaagaaga tcgtattctt ttttcgtcag cgcgatctct ttactgtgca cggttacttt
      421 tttctttgcc ggaaaaacgc ggatgcaccc gtggaggatc tcgttttctc ccccgaagct
      481 gatgcgccgc accacggatt ttatgcgcgc gttgagctcc ggcaggtgaa acggtttcgg
      541 caggtaatca tctgcgccga tgtcgaggcc cgtaatcttg tcgtcgagtg aatttttggc
      601 ggagatgatc acgattcccg ctttcgattt tgctttcttc agctggcgga taatgtcgag
      661 accgttcccg ccgggaaggg tgatgtccac caccacgcaa tcgtaatcat aaatatccgc
      721 cttctcggat cccctgagga aatcttccgc cgtttcaatg acgtttcctt ccttttcgag
      781 gaattggcta atggaattcc ggagaccggg ctcgtcttct accagcagga tcttcatcgc
      841 ataaatgtaa tccgtaattc tgaagtgatt ctggaatcag cgcaggaaaa gtccccagat
      901 atttccgttg gattcttcct tcccgttgac gaaaagcaca ttaccgagca ggatctcttt
      961 cgttttccag tattcctgcc cgtgaaaacc gcggtgtgaa tgcatggagg gcatgcaggc
     1021 aatcctgttc actttcctgt aaagccggtt ggcttcccaa gaacttcgta cccattccgc
     1081 cggggcaacc cgcagttttt ccaggcggac tttttcgccc ctgcgccggg atcccgcaaa
     1141 ttctatcctc atcgggcagt gaaaaaccgg gtcggtgaat ttcgtccggt attcgtaata
     1201 gtaacggatc tctgttgtat ctgaagagtc agcgacaatt ttggtcgtgt aaaaagaatc
     1261 cggtttgctg tcgaagaaag cggcaagctg ccgcggcgtt accgtagagt cgaacggagc
     1321 attgaagaaa agttttttcc tgatgtcacc gagatccggg ctgttgtgca cggcagccgt
     1381 tccggaaagc aggagcaaaa gcagcaggaa tctcattcac ctaatttacg cgaataaccc
     1441 tgcgtgcgga atttcatcgc cccgtcttcg ccgcttcatc gagagcccgg agaaaagttt
     1501 ccaccggctg cgcgcctgaa atgccgtatt tccggtcgat gacaaagaac gggacaccac
     1561 gcgcgcccag ttgcgctgct tcatcagcat cctgtttcac cccggccgag aatgcatcgc
     1621 tgcggagcat gccgcgtatt tcgttttcgt ccagaccaac ttccgcgccg agttgtacga
     1681 gcgtttcagg atcgccgatg ttttttcctt cggtaaaata ggcagcgaac agccgctctt
     1741 ccatcgcatc ctgcaggccg tgctttgcag cgagatgaga aaggcggtgc gcgtcgaatg
     1801 aattggcgac gatcgctttg tcgaaatcgt aatgcagtcc ggccgcttcc gccatgcgca
     1861 cgacgcgttc atgcatcgtt tccgattgct gacggctgat gcccttccgt tccgcgaggt
     1921 aatcgtagat gttctgctcg ccttccggtt tcagcgcagg atcgagctgg taacttttcc
     1981 atgtgacgcg gacggaatct ttttccgcga actgttccag cgctttttca aaatgccgtt
     2041 taccgatgta gcagaaagga cacattacat cggaccatat ttcgatttcc atgatttttc
     2101 gtttagtgga tgaggaaccc gcggttgctg aacgccggga gcagttcctc gaacgtgtgt
     2161 tcgatctgtt cgtctttttc cattacgccg agactgtaca gcatcggcac gtaatgatcc
     2221 ggcgtcggag cggccatgag cccgagcgga cttttttcat aatcgatcag gctccggata
     2281 tcgccttcgt ccagcttttt cttcacccac gaatcgaatt cctcctccca gccgtaaagc
     2341 gtatgatcgc cgccgaacat tttcggagca gcagcacgga gattgtgcac gacggccccg
     2401 ctgccgatga tgagtacgcc tttttcccgc agcggtttca gctggcgtgc gaggtcgaaa
     2461 tgaaagcgcg cactctggta ata
//
LOCUS       DASTSF010000223         2128 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_368685,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000223 DASTSF010000000
VERSION     DASTSF010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_368685"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1518)
                     /locus_tag="VFU15_06490"
     CDS             complement(<1..1518)
                     /locus_tag="VFU15_06490"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008509398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HEU4717459.1"
                     /translation="MKDKLNTLREKIAEAHLGGGKKRIDAQHKKGKLTARERLHFLLD
                     EGSFEEIGMLVTHRATDFGLDKEIYPGDGVVTGYGKINGRLVYVFSQDFTVFGGSLSE
                     THAEKICRVMDLAMKNGAPVIGLNDSGGARIQEGVVSLGGYADIFYRNTLASGVVPQI
                     SAIMGPCAGGAVYSPAITDFILMVENTSYMFVTGPNVVKTVTHEEVTSEELGGAMTHA
                     SKSGVTHFAAANEVDCIQTIRAMLSYMPQNCEEEAPLLEYSPSDEKRPGLNTLIPENP
                     NQPYDIREVITQVIDTDSFLEVHKHFADNIVVGFARLAGRSIGIVANQPAVLAGVLDI
                     HSSTKAARFVRFCDSFNIPLLVFEDVPGFLPGTDQEWNAIISNGAKLLYAFCEATVPR
                     VTVITRKAYGGAYDVMNSKHIGADMNFAWPSAEIAVMGAKGAAEIIFKSEISSAKDPV
                     AKLKEKETEYLEKFANPYMAAARGYVDEVIVPEDTRAKLIAAFEMLKNKADKLPRK"
     gene            complement(1848..>2128)
                     /locus_tag="VFU15_06495"
     CDS             complement(1848..>2128)
                     /locus_tag="VFU15_06495"
                     /inference="COORDINATES: protein motif:HMM:NF026255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEU4717460.1"
                     /translation="YGIIRINAVWMLSFAVKKEFFGDKLSCTIGMDDVFGTMKFHTHS
                     QFGNQDWNYYVSNDTRRINMSISYNFGRVKAEERDVNSNDEEKERLNH"
BASE COUNT          436 a          588 c          591 g          513 t
ORIGIN      
        1 tttgcggggc agcttgtcgg ctttgttctt cagcatttcg aacgcggcga tgagcttcgc
       61 acgcgtgtct tcaggaacga tcacttcgtc cacgtacccg cgggcagcag ccatgtaagg
      121 gttcgcgaat ttctcgaggt attccgtttc tttttccttc agcttcgcca cgggatcttt
      181 cgcagaggaa atctcgcttt tgaaaataat ttccgcggcg cctttagcgc ccatgacggc
      241 gatctcggcg gaaggccagg cgaagttcat gtccgccccg atgtgcttcg agttcatcac
      301 atcgtatgcg ccgccgtagg ctttgcgcgt gatcacagta acgcgcggaa cagtcgcttc
      361 gcagaatgcg tagagcaatt tcgcaccatt ggagatgatg gcgttccatt cctggtcggt
      421 gccgggaaga aaaccgggaa cgtcctcgaa cacgagcagc gggatgttga acgagtcgca
      481 gaaacgcaca aagcgcgcgg ctttcgtgga ggaatggata tcgagcacgc cggcaagaac
      541 ggcaggctgg ttcgccacga tgccgatgct tcttccggcg agacgtgcaa aaccgacgac
      601 gatgttatcg gcgaaatgct tgtgcacctc gaggaaggaa tccgtatcga ttacctgcgt
      661 gatcacttcg cggatgtcgt agggctggtt gggattttcg ggaatgagcg tattcaaccc
      721 tggccgtttt tcatccgaag gcgaatactc gagcagcggc gcttcctctt cacagttctg
      781 cggcatgtag ctgagcatcg cgcggatcgt ctggatgcag tcgacctcgt tcgcagctgc
      841 aaaatgcgtg acaccggatt tcgacgcgtg cgtcatggca ccgcccagct cttcggaagt
      901 gacttcttcg tgcgtgaccg ttttcacgac gttcggcccg gtgacgaaca tgtacgacgt
      961 gttctccacc atgagaatga aatcggtgat ggcgggtgaa tacacagcgc cgcctgcgca
     1021 gggtcccatg atcgcggaga tctgcggaac gacgcctgac gccagcgtgt tgcggtaaaa
     1081 aatatcggcg tagccgccca gcgaaacgac accttcctgg atgcgcgcgc cgccggagtc
     1141 gttgagaccg atgaccggcg caccgttctt catggcaagg tccatgacgc ggcagatctt
     1201 ttccgcatgt gtttccgaaa gcgatccgcc gaagacagtg aagtcctgcg agaacacgta
     1261 cacgaggcgg ccgttgatct ttccgtaacc cgtaacgacg ccgtcgccgg gataaatttc
     1321 cttgtcgagc ccgaaatccg tggcacggtg cgtgacgagc attccgatct cttcgaagct
     1381 tccttcgtcg agcaggaaat ggaggcgctc acgcgcggtg agcttgcctt ttttgtgctg
     1441 cgcgtcgatg cgtttttttc cgccgccgag atgtgcctca gcgatctttt cccgaagtgt
     1501 attgagcttg tctttcatag cgaagtgagg gctaagatac gaatgagtag tgagacgcgg
     1561 gatacccgaa atccgcaggt cgtgacgttc aaccctgcaa atgcccggac cacagacaag
     1621 tttgccgccg ggattcctgt gttgtcgcta tatccgggag ttgccgggaa ttccggcagc
     1681 aagaccaccg gaatttacac gcgtgtcagc gtacgctttt tgcagaaggt catttcacct
     1741 ttcgacacat tgtaaaaatg cctgattatc tgtcatccgg tttttccgaa ccctacatgt
     1801 aaaatggaat ttttccctgc gccgccctgt gaaacccggc ggacgtttca gtggttcagc
     1861 cgttcctttt cctcatcgtt gctgttgacg tcgcgctctt ccgctttcac ccgtccgaag
     1921 ttgtagctga tgctcatgtt tattctccgc gtatcgttcg acacgtaata attccagtcc
     1981 tggttgccga actgcgaatg cgtgtggaat ttcatcgtgc cgaaaacatc atccatcccg
     2041 atcgtgcagc tcagcttgtc gccgaaaaat tctttcttca ccgcaaacga aagcatccag
     2101 acagcattga tgcgtatgat cccgtacg
//
LOCUS       DASTSF010000224         8297 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1375493,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000224 DASTSF010000000
VERSION     DASTSF010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 8297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8297
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1375493"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            393..1436
                     /locus_tag="VFU15_06500"
     CDS             393..1436
                     /locus_tag="VFU15_06500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717461.1"
                     /translation="MYTEKTGHVIVESAGANSNAQVTVSTTYGKFIQKSFTKVRNALG
                     RAEGVNMYMEFEPNERVIDAKKIAFVQSEQEMKGANDPVTDQTHTDASGLGIDQYVQY
                     RNPLYSTGEDLEATKGKPSQGDKLEDYKDRPVRKARFNKEDTAQFHVLGRKKAGWGEF
                     GSRYSMDGKFVSKNATFQDTPFVSGGPEGSSAKFETSVVVADGWQTGLYLGSVTWSWD
                     RTNRKFHDQGIQLKSMGTPSEDFFKAADTWNASGTTGGKKNIPLPMSSGTIKQDKANL
                     YADESGISTGKAAAQLEQSAACRIVDAMVILQSTYLHIRVLDGVHKGAEGWVQEADVD
                     SDKMFPKINNTNP"
     gene            1433..2092
                     /locus_tag="VFU15_06505"
     CDS             1433..2092
                     /locus_tag="VFU15_06505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717462.1"
                     /translation="MISRCFFALLFMVCSSTVCRAKQETDTSCHVAEQRMVKELSSMR
                     VAALYSMADTGLYINNVWKEKENRCAVISILKDREGNDRVQFMAAEILLENCTGIPGE
                     IDRARLGELYAIAIKKNYTGDLNRWGMYDSTDIGPVGKHLLKIGKPAVPALARLLNKR
                     GGLVYSGSKGASVANEWLYRKKDVAAFFIIHIMQYSLPWPKDPKKRDEVIKQVKAKLA
                     E"
     gene            2093..2245
                     /locus_tag="VFU15_06510"
     CDS             2093..2245
                     /locus_tag="VFU15_06510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717463.1"
                     /translation="MSRSFTGTDYMIYNIQKKGSDQKQYTQVRIEQNGRLPLVGWIES
                     SQIRKQ"
     gene            2242..2913
                     /locus_tag="VFU15_06515"
     CDS             2242..2913
                     /locus_tag="VFU15_06515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717464.1"
                     /translation="MNFGFRLYFILCIAVFSSVPVKGQDEKQSPLPDKQMVAWLDSMP
                     YFFGAYYRGDSICQPVWQHGRGKQGLVVITRDSLLSFKARFLAAEILFENDSLYPSDT
                     DRVMLGKLYAAALKGGNAGDADDWGMPFGGEDEFGYIGPHVIRIGKAAVLPFASLLKD
                     KTPMKYMINGKFTDTRYKYGIRVNDFAAFYISHIMGWHYRCLRSRFLRDFRIRHIERK
                     VKRIH"
     gene            2958..4388
                     /locus_tag="VFU15_06520"
     CDS             2958..4388
                     /locus_tag="VFU15_06520"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU4717465.1"
                     /translation="MNETTAPDAAITNAMRFQKEMKWLDRLFRFREYRWRLQRESAMS
                     GGTNEGKSAADDPRYTEVKTLDVFPAAEVEPAVRGNSPYDNFLSHYSFGLAERTALLL
                     SILPHIAPGLLDQYAMNFGVMQGHCPEFGGSRSKNGMLLPTWATLFFLLGGTDMQERA
                     PLFALLDGEHVFRRHRILHFETLQAGTPETEAPLAPELEVTDLLTHGYVRKPVFSNDF
                     PASPLTTNLDWDDLVLSAITKRQITEVKTWLRQKDRLGEELGIGKWLRPGNKILFHGP
                     PGTGKTLTASLLAKFTGKDVFRIDLSMVVSKYIGETEKNLAKVFDKAEHSGWILFFDE
                     ADALFGSRTRVSNSHDRYANQEVSYLLQRIEDYDGVIILASNMKGNIDDAFMRRFNAV
                     VHFPFPKAEERKQLWKNAFSEKIIFDGNISFDSVAGKYELSGGSIVNIAAWCQLMALD
                     NNSYRVTDSMIREGISRELAKEGRTL"
     gene            4788..7082
                     /locus_tag="VFU15_06525"
     CDS             4788..7082
                     /locus_tag="VFU15_06525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717466.1"
                     /translation="MSIAPKSVLESYFLTGEEPTQGQFATLIQSTANLIDDRYLLGLR
                     TYDPTKNYLTGDTTIYGTALYVCTADTTGTFDPLKWSIVASLGAVDYMGTWDTQANDP
                     ALASGVGVKGIYYVVINASSNPDDNTTLDGINDWGTNDWAIFNGTAWQKVDNSQAPVS
                     AAQVSFDPTASISSTDVQDAIVEVDGKFANKQDLLTLTPNYYPFADGAHSLANGVISN
                     QTDGVAIDSGKVIRSSVAGKAQLDFGAGGDTFIVTTDGGGGAEGLLEIQPDSVYIGQT
                     VGQMSIDDTSLNLVHPKIVFNSAQNNFVQLTASTVPYLDASQDLVSSSVTPTELGYLS
                     GVTSALQTQIDSKVDLAGDTMTGPLVLSGDPSVANGAATKNYVDNADASIVTQVNTKV
                     SKAGDTMTGPLILSGNPSLDFGAATKVYVDNADASIVTQVNTKVSKAGDTMTGPLILS
                     GNPSLDFGAATKVYVDNGLLTKLSLSGGTMTGALTLNGDPSSSLEAATKNYVDNADAS
                     IVTQVNTKVSKAGDTMTGPLILSGNPSLDFGAATKVYVDNGLLTKLSLSGGTMTGAIT
                     LPGDPSSALQAATKQYADTKVPLAGGTMTGNLVLFSTGTPPTDASATSKAYVNSLIGG
                     IPAQVKVISLPFTFDGAYSISTPVDTWGRVPLEITTPFTNDVFPGATTITGKLTIDYS
                     TSTNSTTGRVCLLEWHNGLAVFNPVSGTDNAVAATNASWIRQSFSFSAGILNAATTYS
                     IMIMRQGSSGSMLIESATLSITYS"
     gene            7092..7406
                     /locus_tag="VFU15_06530"
     CDS             7092..7406
                     /locus_tag="VFU15_06530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717467.1"
                     /translation="MKDITEIFGTNFMLTGDNIDPRDLDFTFYTHEKITNNPDGTPQM
                     KQYYMNYDEGTDTFSDLAVECDYTYTYDAGVLTKRVEDINWYFVDGTTVGVTRQLIQV
                     YS"
     gene            7430..8044
                     /locus_tag="VFU15_06535"
     CDS             7430..8044
                     /locus_tag="VFU15_06535"
                     /inference="COORDINATES: protein motif:HMM:NF025431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4255 domain-containing protein"
                     /protein_id="HEU4717468.1"
                     /translation="MIDDALNFLTDEINAYLQLKNDPVSATAPEIVLTNVAAEDGNWA
                     IPPRTLGLSLINIEEDRIYKDQQTAFRNENNDIEHYNPNIKLNLYILISANFASGDAG
                     GGTNSTGVYGEGLKQLSYVISFFQGKYVFTPDNSPNMSPALNKLIVELYSYSFEQQYN
                     FWAVIGAKYLPSILYRVRLLTYQEKRLLDQQQPVTDLNITTSQN"
     gene            8046..>8297
                     /locus_tag="VFU15_06540"
     CDS             8046..>8297
                     /locus_tag="VFU15_06540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717469.1"
                     /translation="MSSTIFNKAFSLELSHDFYVVASKYLVNKDLTFAPTKECSALMQ
                     KGRMRFVPTPKGAVVFYQSYIDGTDTVLPLVELADGSEFV"
BASE COUNT         2154 a         2136 c         2100 g         1907 t
ORIGIN      
        1 cgcctatgcc ggcgctttcg accttccaca cacagttggc gggaacagtg atcgattcat
       61 cggtgctggg cgggagcgtc atcacgaaga cgcgcgaaaa aatgagctga ggtacaggca
      121 tggtcgtatg gatttgatga attcactttt tgactcaacc ggctaaaata gttcctgcgc
      181 ccccgcgtgc aaggattttc tttttcgcga tttttttcaa cgtgtaaatt tcacgcccct
      241 ccctgcacag tctctttctt tatttccagg tcgttagaat gtgtgccagg aatttttcgc
      301 gtataaaaac gaaaaaattt gtccgcggaa ccgaatgcgt atttttttac atttaatgat
      361 tgtctgctgc ttaactttta aacgaccggg aaatgtatac cgaaaaaaca ggtcatgtca
      421 tagtagaatc ggccggcgca aacagcaatg cacaggttac cgtttccact acctacggta
      481 aattcatcca gaagagcttc accaaagtga gaaacgccct gggtcgtgcg gaaggtgtga
      541 acatgtacat ggaatttgaa cccaatgagc gggttatcga tgcgaagaag atcgcctttg
      601 ttcagtcgga acaggaaatg aaaggcgcca acgatccggt caccgaccag actcataccg
      661 atgcttccgg acttgggatc gaccagtacg ttcagtacag gaacccactg tactcaacag
      721 gtgaagacct ggaagcaact aaaggaaaac cgtcccaggg agataagctt gaagattata
      781 aagatcggcc cgtccggaaa gccaggttca ataaagaaga cacggcccag ttccatgttc
      841 tcggaagaaa aaaggccggc tggggggaat tcggcagccg gtatagtatg gacggaaaat
      901 ttgtgagcaa gaacgccacc ttccaggata cgccatttgt gtcgggcgga ccagagggtt
      961 ccagtgcgaa atttgaaacc tccgtggtcg ttgcggatgg atggcagaca ggcttatacc
     1021 tcgggtccgt tacatggagc tgggatcgca cgaaccggaa atttcacgac cagggaattc
     1081 aactgaaatc gatgggaacg ccctctgaag attttttcaa agcggctgat acctggaatg
     1141 cttcgggcac aaccggcgga aagaagaata ttcccttacc catgagtagt ggaaccataa
     1201 aacaggacaa ggcaaacctg tacgcggatg aatcggggat cagtaccggg aaagcagctg
     1261 cgcaactgga acagtccgca gcatgcagaa ttgttgacgc gatggttatc cttcagtcga
     1321 cttacctcca cataagagtt ctggatggcg tccacaaagg ggcggaaggc tgggtgcagg
     1381 aagcggatgt tgattctgat aagatgttcc cgaaaataaa taatacgaat ccgtgatcag
     1441 tcgctgtttt ttcgcgctcc tgttcatggt ttgctcttca accgtgtgcc gggcaaagca
     1501 ggaaacagat acctcctgcc atgttgcaga acaacgaatg gtgaaagaac tttcctccat
     1561 gcgggttgcc gctttgtatt ccatggcaga tacgggacta tacatcaaca acgtatggaa
     1621 ggaaaaagaa aaccgttgcg ctgtcatttc tattctgaaa gaccgggaag gaaatgaccg
     1681 tgtgcaattc atggcggcag aaatcctgct agaaaattgc accggtattc ccggggagat
     1741 cgatcgtgca agattgggtg agctctacgc gatcgccatt aaaaaaaatt ataccggcga
     1801 cctcaaccgg tggggaatgt acgattcaac ggacatagga ccggtgggaa aacaccttct
     1861 gaaaatagga aaaccggccg ttcccgcgct cgccaggctt ttgaataaaa gaggaggatt
     1921 ggtctattcc ggcagcaaag gagcttctgt ggccaacgag tggttatacc ggaaaaaaga
     1981 cgtcgctgca tttttcatca ttcatattat gcaatattcc ctgccctggc cgaaagaccc
     2041 gaaaaaaaga gacgaggtga taaagcaggt caaagcaaag cttgctgaat gaatgagccg
     2101 ttcatttacc ggaaccgatt acatgattta caacatacag aaaaaaggca gcgatcagaa
     2161 gcaatataca caggtgcgca tcgaacagaa cggacgtttg cccctcgtcg ggtggatcga
     2221 atcgtcccaa atccggaagc aatgaatttc gggttccgct tgtattttat tctatgcatc
     2281 gctgtatttt catccgttcc ggtgaaaggc caggacgaaa aacaatcgcc gctccccgat
     2341 aaacagatgg tcgcatggct cgattccatg ccctattttt ttggcgccta ttaccgcggc
     2401 gattccatct gtcagccggt atggcagcac ggcagaggaa aacagggtct cgttgtcatt
     2461 acccgcgact cgctgctttc gttcaaggcg cgttttctcg ccgctgaaat tttatttgaa
     2521 aacgattccc tttatccttc cgacaccgat cgcgtcatgc tgggaaaact atacgccgca
     2581 gcattgaagg gcgggaatgc gggcgacgcg gacgactggg gaatgccgtt cggtggggaa
     2641 gacgaattcg ggtacatcgg cccgcacgtc atccggatcg ggaaagcggc tgtgctcccg
     2701 ttcgcatcgt tgctgaagga caaaaccccg atgaagtaca tgataaacgg gaaattcacg
     2761 gataccaggt acaagtacgg gatccgcgtc aacgatttcg ctgcgttcta catcagccac
     2821 attatgggct ggcattaccg ttgcctgcgc tcacgatttt tgcgggattt ccgcatccgt
     2881 cacattgaac gaaaagtgaa aaggatccat tagcaactca cctgttccgg tcgcaccggg
     2941 aatttatgct tcaactcatg aatgaaacca ccgcacccga cgccgccatc accaacgcga
     3001 tgcgctttca gaaagagatg aagtggctcg accgcctttt ccgcttccgc gaataccggt
     3061 ggcgcctgca aagagaatcg gcgatgagcg gcggaacaaa cgaaggtaaa tccgcagccg
     3121 atgatccgag gtatacagaa gtaaagaccc ttgacgtctt ccctgcagct gaagtggaac
     3181 cggccgtgcg cggcaattct ccctatgata attttctgtc acattacagc ttcgggctcg
     3241 ccgaacgcac ggcgcttctt ctgtccatcc ttccgcatat cgcaccggga ctgctcgatc
     3301 agtatgcgat gaatttcggc gtgatgcagg gacattgtcc cgaattcgga ggcagccggt
     3361 cgaaaaacgg gatgctcctc ccgacctggg cgactctttt ttttcttctc ggcggaacgg
     3421 acatgcagga acgtgctccg ctcttcgcac ttctcgacgg agagcatgta ttccgaaggc
     3481 acaggatcct tcattttgaa acgctccagg ccggaacgcc cgaaaccgaa gcgccactgg
     3541 cgccggagct ggaagtcacc gacctgctga cgcacgggta cgtgcggaaa ccggttttca
     3601 gcaatgattt cccggcttcg ccgctgacca cgaacctcga ctgggacgat ctcgttttgt
     3661 cggcgataac aaaacgccag atcactgaag tgaaaacatg gctgcgtcag aaagaccgcc
     3721 tgggcgaaga gctgggtatc ggaaaatggc tgcggccggg aaacaaaata ctttttcatg
     3781 gcccgccggg caccggcaaa acgcttactg cttcgctgct ggctaaattc acgggcaaag
     3841 atgttttccg catcgacctt tccatggtag tgtcaaaata catcggcgaa acggaaaaga
     3901 accttgcaaa agtgttcgac aaagcggaac attccggctg gatacttttt ttcgatgaag
     3961 cggatgcgct gttcggttcg cgcacgcgcg tgagcaattc ccacgaccgt tatgccaacc
     4021 aggaagtgag ctatcttctc cagcgcatcg aagattacga cggcgtaatt attctcgcaa
     4081 gcaacatgaa aggaaatatc gacgatgcgt tcatgcgcag gttcaatgcc gtcgttcatt
     4141 tcccgttccc gaaagcggaa gaaagaaaac agctctggaa aaatgcgttt tcggaaaaaa
     4201 taattttcga cggtaacatc agtttcgatt cggtcgccgg gaaatatgag ctctccggcg
     4261 gaagcatcgt gaacattgcc gcctggtgcc agctgatggc gctcgacaac aattcctacc
     4321 gggtaacgga cagcatgatc cgcgaaggga tttcccgcga gctcgcaaaa gaagggcgca
     4381 cgctctgatc cgtttacgtt tccttcccgg atttaatcat tgacactcgc ttacgtcacg
     4441 gcagaacttt cacctttctt cgacagcgat tccgctttat caattttttc ccgctgaatt
     4501 ttttctttcc cgtcatttca tcttcatgtt ttcgttaagc gtgataaaaa tatttctgct
     4561 taaaaaaagt tacgtgtaaa aaaacagggg gtacttgcac agccttcccg ttcatttcca
     4621 gcacattata acgtatgcca agaatttttc ggctttgaaa tcgaaaaaat ttgtccgtgc
     4681 aaaagtttta tgaatggatc aactttgaac ggatcaaaaa aagaactccc gctctttctg
     4741 atcacatcaa cggaaaattt acgtgtaatt cccaataaga accgagcatg tcgatagcac
     4801 ccaaatccgt cctcgaatcc tatttcctga ctggcgaaga acctacacag ggacaattcg
     4861 ccacgctgat ccagtcgacg gccaatctca ttgacgaccg ttacctgctc gggttgcgga
     4921 cgtacgatcc caccaaaaac tatcttaccg gcgataccac catttacggc actgcacttt
     4981 atgtctgcac ggcggacacg accggtacgt tcgatccgct gaagtggtcg atcgttgcat
     5041 cgctgggcgc cgtcgactac atgggcacct gggatacgca ggcgaacgat cctgcgctcg
     5101 cgagcggtgt aggtgtaaaa ggaatttatt atgtggtcat caatgcaagc tctaatcctg
     5161 acgacaatac cacgcttgac ggcatcaacg actggggaac gaacgactgg gcgatcttta
     5221 acgggaccgc atggcagaaa gtagataatt cgcaggcgcc cgtgtctgcc gcacaggtaa
     5281 gcttcgatcc gaccgcttcc atttcgtcca cggatgtgca ggatgccatt gttgaagtgg
     5341 atggaaaatt tgcgaataag caggatcttc tcacgctcac gcccaattat tatccgttcg
     5401 ctgacggcgc tcattcgctg gcaaacggcg tcatctccaa tcaaacggat ggcgttgcca
     5461 tcgacagcgg aaaagtgatc cgcagctctg tggccggcaa agcgcagctg gacttcggtg
     5521 caggcggcga cactttcatc gtcactacgg atggcggcgg cggtgccgaa ggtcttctcg
     5581 aaatacagcc tgattccgta tacatcggtc aaacggtcgg ccagatgtcg atcgacgata
     5641 cgtcgctcaa cctcgttcac ccgaagatcg ttttcaattc cgcgcaaaat aattttgtcc
     5701 agctcaccgc atccacggtt ccgtacctcg atgcatcgca ggatttggtc tcgtcctccg
     5761 ttacgcctac cgagctcggc tatctctccg gtgtaacatc agcgctgcaa acgcagatcg
     5821 attcgaaggt cgatctcgca ggtgatacca tgaccggtcc gctggttctc agcggagatc
     5881 cttccgttgc gaacggcgcc gctacgaaaa attatgttga taatgcggac gcctctattg
     5941 tcacccaggt aaataccaaa gtgagcaaag ccggcgacac gatgacgggt ccgcttatcc
     6001 tcagtggaaa tccttcgctc gatttcggtg ccgcaacaaa agtttatgtc gataatgccg
     6061 acgcctctat tgtcacccag gtgaatacca aagtgagcaa ggccggcgac acgatgacgg
     6121 gtccgcttat cctcagcgga aatccttcgc tcgatttcgg tgcggcaaca aaagtttatg
     6181 tcgacaacgg gctgctgacg aagctgagcc tttccggcgg gacaatgacg ggagcgctga
     6241 cgctcaatgg agatccgtct tcatcactcg aagctgcgac taaaaattat gtcgataatg
     6301 ccgatgcttc tattgtcacc caggtaaaca ccaaggtgag caaggccggc gacacgatga
     6361 cgggtccgct tatcctcagc ggaaatcctt cgctcgattt cggtgctgca acaaaagttt
     6421 atgtcgacaa cgggctgctg acgaagctga gcctttccgg cggaacgatg accggggcga
     6481 tcacgcttcc gggcgatcct tcctccgcgc tgcaggcagc cacgaagcaa tacgccgata
     6541 cgaaagttcc gcttgcgggc gggacgatga ccgggaatct tgttcttttc agcacaggca
     6601 caccaccgac ggacgcgtcc gcaacttcaa aagcttacgt gaattcgctt atcgggggaa
     6661 tcccggcgca ggtgaaagtg atctcgctgc cgttcacgtt tgatggcgcg tactcgattt
     6721 caacaccggt tgatacatgg ggacgtgtgc cgctggaaat caccacacca ttcacaaacg
     6781 atgtatttcc cggcgctaca acgatcaccg gcaagctgac catcgattac agtaccagca
     6841 cgaactcaac aacaggacgc gtctgcctgc ttgaatggca taacggcctt gcggtcttta
     6901 acccggtcag cggaacggac aacgccgtcg ctgcgacaaa cgcctcctgg atcaggcaat
     6961 cgttcagttt ttcagcgggc attttgaatg ccgccacaac ctattccata atgatcatgc
     7021 gccagggaag ctccggctcc atgctcattg aaagcgctac actttccatt acctattcat
     7081 aagaatttac aatgaaggac atcacggaaa ttttcggcac caatttcatg ctcacgggag
     7141 ataatattga cccccgcgat cttgatttca cgttctatac acatgaaaag atcacgaaca
     7201 atcccgacgg aacgccgcag atgaagcagt attacatgaa ctacgatgag ggaacggata
     7261 ccttttccga tctcgccgtg gaatgtgatt acacgtatac gtacgacgcc ggtgtgctga
     7321 cgaaacgcgt ggaggatatc aactggtatt tcgtagacgg aacgacggtt ggtgttacac
     7381 ggcaacttat ccaggtttac tcctagtaat tatccgaagg gaaagaaata tgatcgacga
     7441 cgcgctgaat tttcttacgg acgagataaa cgcttatctc cagctgaaga acgaccctgt
     7501 ttctgcaacg gcgcctgaga tcgtattgac gaacgtggcc gctgaagacg gcaactgggc
     7561 gataccgccc cgtacgcttg gtctttcgct gatcaatatt gaggaagaca ggatttataa
     7621 agatcagcag acggcattcc ggaatgagaa caatgatatt gagcattaca atcccaacat
     7681 caagctgaat ctctacatcc tgatcagcgc caatttcgca tcgggcgatg caggcggcgg
     7741 aacgaattct acaggtgttt acggagaagg attgaagcag ctatcgtatg tcatcagctt
     7801 cttccagggc aagtatgttt tcacgccgga caattcaccg aacatgagcc cggcgctgaa
     7861 caagctgatc gtggaactgt attcctattc gttcgaacag caatataatt tctgggcggt
     7921 gatcggtgcg aaatacctgc cgtcaattct ttaccgcgtt cgcctgctta cttaccagga
     7981 aaaacgcctg cttgaccagc agcaaccggt gaccgatctg aacattacga catcacagaa
     8041 ctgaaatgag ttcgaccata ttcaataaag cattttcgct ggagctctcg catgattttt
     8101 atgtggtggc gagcaaatac cttgtgaaca aggacctgac cttcgctccc acgaaagaat
     8161 gcagcgcgct gatgcaaaaa gggagaatgc ggtttgttcc cactcccaaa ggcgcagttg
     8221 tgttctacca atcctatatc gacggaacgg atactgttct gccgctggtg gagctcgctg
     8281 atggttctga atttgtc
//
LOCUS       DASTSF010000225         4338 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_36340,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000225 DASTSF010000000
VERSION     DASTSF010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4338
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36340"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..864)
                     /gene="lipA"
                     /locus_tag="VFU15_06545"
     CDS             complement(<1..864)
                     /gene="lipA"
                     /locus_tag="VFU15_06545"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HEU4717470.1"
                     /translation="MSENVESNSSVTRIKKPDWLRVKLPVGENYLRVRGLVDQYKLHT
                     ICESGNCPNMGECWGAGTATFMILGNICTRSCGFCAVATGRPASVDWEEPERVAQSVK
                     LMGVKHCVITSVDRDELKDGGSTIWKMTIEAIRRISPETTVETLIPDFKGEKENIQRI
                     IDVMPEIVSHNMETVRSLTKKVRIQAKYDRSLGVLKFLREGGCRTKSGIMVGLGEKDE
                     EVFETMDDLVNAGVEIFTIGQYLQPTKKHLPVVEFVHPEKFALYRQVALEKGFRYVES
                     GPLVRSSYHAEK"
     gene            complement(871..1653)
                     /locus_tag="VFU15_06550"
     CDS             complement(871..1653)
                     /locus_tag="VFU15_06550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717471.1"
                     /translation="MRRITLLMIVFLAAGKIFAQVDDQTQTQTVKVNSNVYDDTKLLY
                     RNERCGGLVIHSSGFGFDFRRGWHVTGAKKRVLEFELVNFRNIKEVKVSNPYLDHPKG
                     YYYGKLNSLAIFRPGVGYQQVIFSKPEKNGVEIRYVTFVGMSLGFAKPVYLEILEDTP
                     QQQVKIVVTEKYDPAKHFVDNIYGRAPWPRGLGETKIYPGGYAKFGFNFEYGALDDDV
                     KSIEVGLCVDAYPKVIPLMANEHNHQVLLSLYLNFSYGHKWF"
     gene            1715..2119
                     /locus_tag="VFU15_06555"
     CDS             1715..2119
                     /locus_tag="VFU15_06555"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEU4717472.1"
                     /translation="MKTSEVVYTGGLRTSCVHLRSGQTIITDAPPDNNGKGEAFSPTD
                     LLSTSLACCMLTIMGIVSGRHGIDIDGTKAEVTKIMADQPRRVSEIIVEFVMPRSYTE
                     KEKQLLENAARTCPVAKSLHPDLVQNIKFSWK"
     gene            complement(2166..2303)
                     /locus_tag="VFU15_06560"
     CDS             complement(2166..2303)
                     /locus_tag="VFU15_06560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717473.1"
                     /translation="MKKLIAAFLLIAFSGLTLVSCKSKQHCDAYQGSGSIQPHHKKAN
                     S"
     gene            2484..3176
                     /gene="ung"
                     /locus_tag="VFU15_06565"
     CDS             2484..3176
                     /gene="ung"
                     /locus_tag="VFU15_06565"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005936478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEU4717474.1"
                     /translation="MSASTTSASPQIDQSWKDNLLDEFNSPYFQNLKTFLTEEKKQYP
                     VYPPGPLIFSAFNQTPFDAVKVVILGQDPYHGAGQANGLCFSVSQGIKHPPSLVNIFK
                     EMQKDLNVPYPKTGDLSGWAKQGVLLLNTTLTVRAQTPASHQGQGWEEFTDAVIRSLS
                     EKREGIVFILWGRHAQNKKALIDTSKHFILEAAHPSPYSANGFFGCRHFSKANYLLMQ
                     SGQAPINWLALG"
     gene            3274..>4338
                     /locus_tag="VFU15_06570"
     CDS             3274..>4338
                     /locus_tag="VFU15_06570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717475.1"
                     /translation="MLSIQKAVLLLDEFHLAEFKRHLEESRAELPLRLVDATLKIGWE
                     ENDSDDLCKAIYKKAEPQEKRKFFQLAHHTFKLTGFLSRNYPSYLLHNISRIEKLINE
                     GKQKEAFRLAEVLLDVSEKTEDYTTAKSVLRFLAQHSYMREKKSEAVRYLQRNAQLID
                     NERALNSIYLYMRSHLHFKDKSTTTEGETEKHLSFFAQFHGSESFAVRILGRYGSCYT
                     LHFLNDDRFYSKETLDELNALVDELEKAPYVIFSFSDDIELNIEYLKLKLLVGWLNPD
                     ELQKAAAEILRKRETPGFWKNYINGAQIAFLSIQASVLVSRYAKGYRKNWNEDLPADI
                     REQVSFYRKACEEILSHPSWE"
BASE COUNT         1050 a         1178 c         1099 g         1011 t
ORIGIN      
        1 tttttctgca tgatagcttg aacggacaag cgggccgctc tcgacataac ggaatccctt
       61 ttcgagcgcc acctgcctgt acagagcaaa tttttcaggg tgaacgaatt caaccaccgg
      121 aagatgcttt ttggtcggct gaaggtattg ccctatcgtg aatatctcca cgccggcatt
      181 cacgaggtcg tccatcgttt caaaaacttc ctcgtccttt tctccgagcc cgaccatgat
      241 accggatttg gtgcggcaac ctccttcgcg gaggaatttc agcaccccga gactgcggtc
      301 gtatttggcc tggatgcgca cttttttcgt caggctccgg accgtttcca tgttgtgcga
      361 aacgatctcc ggcatgacgt cgatgatgcg ctgtatattc tctttttcac ctttgaaatc
      421 cgggatcagc gtttcgacgg tcgtttccgg gcttatcctg cggatggcct cgatcgtcat
      481 tttccagatc gtggaaccgc cgtccttcag ctcatcccgg tcgacggatg tgatcacgca
      541 atgtttcacg cccatcagct tcacggattg cgccacacgc tccggctctt cccagtcgac
      601 ggaagccggg cggccggtgg caaccgcgca gaagccgcat gatcttgtgc agatgttccc
      661 gaggatcatg aaagtggctg ttccggcgcc ccagcattca cccatgttcg ggcagttccc
      721 gctttcgcag atcgtgtgca gcttatactg gtccacgagt ccgcgcacgc gcaggtaatt
      781 ctcacccacg ggcagcttca cgcgcagcca gtcgggtttt tttattcttg ttacggaaga
      841 attgctttcc acgttttcgc tcatcgcagc tcagaaccac ttatgtccgt aggaaaaatt
      901 caggtaaagc gaaagcagca cctggtggtt gtgctcattc gccatcagcg gaataacctt
      961 cggataggca tcgacacaga ggccgacctc gatgcttttc acatcatcgt ccagcgcgcc
     1021 gtattcgaag ttgaacccga atttggcgta accgcccggg taaatttttg tttcacccaa
     1081 cccgcgcggc cagggtgcgc ggccgtaaat attatccaca aaatgttttg ccgggtcgta
     1141 tttttccgtg acgacgatct tcacctgctg ctggggcgtg tcttcgagta tctcgaggta
     1201 aaccggcttg gcaaaaccga gcgacattcc cacaaacgta acgtagcgta tctcgacgcc
     1261 gttcttttcc ggcttggaaa aaataacctg ctggtacccc acgcccggcc ggaagatggc
     1321 caggctgttg agcttgccgt aataatagcc tttgggatga tcgaggtaag gattggaaac
     1381 tttcacctct tttatgttcc ggaaattaac gagttcgaac tcgaggacgc gttttttcgc
     1441 tccggtcacg tgccagcccc tgcggaaatc gaacccgaaa ccgctggaat ggatgacaag
     1501 gccgccgcag cgctcattac gatacagcag cttggtgtcg tcgtaaacgt tggaattgac
     1561 cttaaccgtc tgtgtctgcg tctggtcgtc gacctgggcg aaaatttttc ccgcggcgag
     1621 aaacacgatc atgagcaggg tgatgcggcg catcttttca gctaattttg gatagaatat
     1681 caaagttaat aataaaaaga ccaaaacaag tatcatgaag acatcggagg tcgtttacac
     1741 gggagggctt cgcacgagct gcgtacatct ccgttcggga caaaccatta ttacagacgc
     1801 gccgcccgac aacaacggca aaggcgaagc attttcaccc accgatcttc tttccacctc
     1861 gctggcctgc tgcatgctca cgatcatggg aattgtaagc gggcgccacg gcatcgacat
     1921 agacggaacg aaggcggaag tgacgaaaat aatggccgat cagccgcggc gcgtttcgga
     1981 aattatcgtg gaattcgtca tgccgcgttc ctacacggaa aaggaaaaac agctgctgga
     2041 aaatgcggcg cggacatgcc cggtggccaa aagccttcac ccggaccttg tgcagaacat
     2101 taaattcagc tggaaataaa aagtcccgcc atgcgggact ccttcatgaa gctgcgaaaa
     2161 gcagatcagg aattggcttt tttgtgatgg ggctgaatag agccggagcc ctggtaagcg
     2221 tcacagtgct gcttggattt gcatgaaaca agcgtcagtc ctgagaaagc gatgagcaga
     2281 aaagctgcaa ttaatttttt cattggaatc aggtttcggt ttcaggtggt tttacaaata
     2341 ccgtgcaaaa gtacccttcc gggccatgcc aagcaaatgc gcgatttatt tttttattaa
     2401 caagacgaaa attttacatt tgcaaacctt ccatttacac tatttacagg cccgtttttt
     2461 tatacctact ttttttatta caaatgagcg cttctactac ttctgcttcc ccgcagatcg
     2521 accagagctg gaaagataac ctgctcgacg aattcaacag tccgtatttc cagaacctga
     2581 agacgttcct tacggaagaa aaaaaacagt acccggttta tcctcccggt ccgcttattt
     2641 tcagcgcatt caaccagacg ccgttcgacg cggtgaaagt ggtgatactc ggacaggatc
     2701 cttatcacgg ggccggccag gcgaacgggc tctgcttttc cgtttcgcag ggcatcaagc
     2761 atcctccgtc actcgtcaat attttcaagg agatgcagaa agacctcaat gttccgtacc
     2821 cgaaaaccgg tgatctttcc ggctgggcga aacagggcgt gctgctgctg aacactacgc
     2881 ttaccgtgcg tgcgcagacg ccggcgtcgc accagggaca gggatgggaa gagttcaccg
     2941 acgcggtgat ccgcagcctg tccgaaaaaa gagaaggcat tgttttcatt ctctggggac
     3001 gtcatgcgca gaacaaaaaa gcgctcatcg acacgtcaaa acattttata ctggaagctg
     3061 cgcacccttc gccttattcg gcaaacggtt ttttcggctg ccgtcatttt tccaaagcaa
     3121 attacctgct catgcagtcc ggccaggcgc ccattaactg gcttgcactg ggctgatctt
     3181 ttcccgcccc gctgtcatct cccgcgacag cctttacgaa tgaatttccc ttccgggcaa
     3241 gggagaaccg attgctgtac ctgaccgaaa actatgctga gcattcagaa agctgttctt
     3301 ctgctggatg aatttcatct cgcggaattc aagaggcacc tggaggagtc gagggcagag
     3361 ctgccgctgc gcctggtgga tgccacgctg aagatcggct gggaggaaaa cgattcggac
     3421 gatctctgca aagccattta caaaaaagcg gagccccagg aaaagcggaa attcttccag
     3481 ctggcgcatc acacattcaa gctgacggga tttctcagcc gcaattatcc atcctacctg
     3541 ctgcataaca tttcccgcat tgaaaagctg atcaacgaag gcaaacagaa agaagcgttc
     3601 cggctcgcgg aagtgctgct cgacgtttcg gagaaaacgg aagactacac gacggcaaaa
     3661 tccgttctcc gtttcctggc gcagcattcg tacatgcgcg agaaaaaatc ggaagccgtg
     3721 cgttacctgc agcgcaatgc acaactgatc gacaatgaac gcgcgctcaa ttcgatctac
     3781 ctgtacatgc ggagccacct tcatttcaag gacaaatcca ccacgaccga aggcgaaacc
     3841 gagaagcatc tttccttttt cgcgcaattc cacgggagcg aatcgttcgc ggtgcgcatt
     3901 ctcgggcggt acggctcctg ctacacgctt catttcctga acgacgaccg cttctattcg
     3961 aaggaaacgc tggacgagct gaatgcgctg gtggacgagc tcgaaaaagc gccgtacgtt
     4021 atcttctctt tttcagacga catcgagctg aacatcgaat acctcaagct gaagctgctg
     4081 gtgggatggc tgaatcccga cgagctgcag aaagcagccg cagaaattct ccgcaagcgc
     4141 gaaacgccgg gcttctggaa aaactacatc aacggcgcgc agatcgcgtt cctgagcata
     4201 caggccagcg tgctggtgag ccgttacgcg aaaggctatc gcaagaactg gaatgaagat
     4261 cttcccgccg acatcaggga acaggtcagc ttttacagga aggcctgcga ggaaatcctt
     4321 tcccacccga gctgggaa
//
LOCUS       DASTSF010000226        19393 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_798945,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000226 DASTSF010000000
VERSION     DASTSF010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 19393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19393
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_798945"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1553
                     /locus_tag="VFU15_06575"
     CDS             <1..1553
                     /locus_tag="VFU15_06575"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HEU4717476.1"
                     /translation="SPWGLCIGDYNNDGYNDVFQGSSSNGYLLTSSNATSFTLQNMTT
                     TFGGGSVFTQGCNFADIDNDGFLDLFICHDTGKPKVYMGDGTTGGWTFDQSVMPLSQY
                     PILINSDSNSGNYASIWTDVNNDGYIDMMITHCRQSITNSSDPRRIDQIFINNANGTY
                     TQDTSNWTGLRDGAQGWSTAWGDIDNDGDMDAFVLNYDVNSKVMINDGNGHFTDVMAT
                     SGIANTTTYFGENASFHDFDNDGFLDLLITGDQHYLYQGHGDGTFTLVSTQPFDYWNA
                     SHTTFRYIRGTGVGDLNGDGFLDVYASYANLYNSATTSKNDHLWMNTCVGNGNHWIKF
                     NLVGGAAPGMSNKNGVGSIVKIYGPWGVQVREVRSGEAYGLQNSLSVYFGLGQNTAID
                     SAVVIWPSGIVDELLTLNGDNSYTVMEGGNPTSTHPVEQKALSMAVYPTPASDYVNIR
                     LDNFAQYGLNNLSLKIYDGTGKLVYSEAALQTCIVTIGKDILSSGIYMVEIDNKEKRI
                     ASDKLIMQ"
     gene            1638..3410
                     /locus_tag="VFU15_06580"
     CDS             1638..3410
                     /locus_tag="VFU15_06580"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HEU4717477.1"
                     /translation="MKKFVLAPAFLLPFFAAAQNVISFSDQSTLLPTSNFHSGNSIGV
                     VDMNGDKKDDIVRASNNATMYIEYQNAPNATFSENSFANSIGDPWGMCVGDLNNDGYN
                     DVFWGDYGNTWVLVRNSATTYTGTNIAATLYPGFIFVQGCNFADINNDHFLDLFVCND
                     DYSPHIYVNNNGTGWTFNQGLMPLATFPSSDGSGNYASIWTDINNDGLTDCMVTHCRQ
                     GVTSSSDARRIDQVFLNNGNGTYTQDITNWTGLRDGAQGWSTAWGDIDNDGDMDAFVL
                     NYDVNSTLMINDGNGVFTNVMSGSGINNTTTIFGENATFQDFDNDGYLDLMISGDHHL
                     VYHNNGNHTFTLQATDPFPYAGHTITAHAVGDLNGDGWLDVYASYCDIYQSANSSRND
                     KLWMNNTNNGNHYVMFDLVGGAINGFSNKNGVGASVKIYGPWGVQVREVRSGEAYGIQ
                     NSMCCHFGLGSNTTVDSVVVRWPSGIVDVMTNLPCDQHYTINEGMSPTATAYLSEKPV
                     SLAVYPNPVSDVASIRVQNFAQYGLNNLSLNIYDVNGKLVYSEAELQQSIIMIGRDVL
                     SSGLYFVELKNKDKRLASERMMVE"
     gene            complement(3613..4176)
                     /gene="frr"
                     /locus_tag="VFU15_06585"
     CDS             complement(3613..4176)
                     /gene="frr"
                     /locus_tag="VFU15_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012780295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEU4717478.1"
                     /translation="MNEDLQLIIETAQEAMDHAVKHLEAELVKVRGGRANAGMLDGIH
                     VDYYGVNTPLSQVANVNTPDARTISVQPWEKGMLDPVLKAIQAANLGFNPTNNGTLII
                     CAIPPMTEERRKALVKKAKEEGEHAKVSIRTARRDANEEVKKLQKNGLPEDEAKEGEA
                     KVQTITDNFIAKVDKHLEAKEKEIMTV"
     gene            4285..4683
                     /locus_tag="VFU15_06590"
     CDS             4285..4683
                     /locus_tag="VFU15_06590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717479.1"
                     /translation="MKFIKSFAAIVIAFVLVAGASSCKGGSAFKVGDHVAAKWTNNNY
                     YLGSITAVNADKYSILYDDGDKLDVPVTDLKTLCNQSDLKVGDKVMASWNSSVMLYPG
                     TVTELQSGGAMVKWDDGSEPSLSPYGKLTK"
     gene            complement(4745..5452)
                     /gene="pyrH"
                     /locus_tag="VFU15_06595"
     CDS             complement(4745..5452)
                     /gene="pyrH"
                     /locus_tag="VFU15_06595"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007653646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEU4717480.1"
                     /translation="MKYKRILLKLSGEALMGEKQFGIDHEILNQYAQEIKSVVAAGVE
                     VAIVIGGGNIFRGIQAADGGMDRVQGDYMGMLATMINSMALQAALEKLEIPTRLQSAI
                     EMKQICEPFIRRRAVRHLEKNRVVIFGSGTGNPYFTTDTAASLRAIEIEADVVLKGTR
                     VDGIYTADPEKDKTATKFETITFNEVYEKGLNVMDMTAFTLCNENKLPIIVFDMNRPG
                     NLSRVVTGEKVGTLVAF"
     gene            complement(5512..6333)
                     /gene="tsf"
                     /locus_tag="VFU15_06600"
     CDS             complement(5512..6333)
                     /gene="tsf"
                     /locus_tag="VFU15_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HEU4717481.1"
                     /translation="MTATITAADVNKLRTMTGAGMMDCKKALTETNGDFDAAIDLLRK
                     KGQKVAANRSDRDAKEGQVVAKTNGGRGVVIVLNCETDFVAKNDDFAKVADTFAQIAL
                     DKAPADLAALLALPFESTTIGEKVIDLVGKIGEKIELSRYEQISAAKVVAYNHPGNKL
                     ACIVGFNKEASDDMAKDVAMQVAAMAPVAVDKDDVDTTTLERELDIAREQVRAEGKAE
                     DMVEKIAQGKLNKFYKESTLLNQEFIKDSKKSIRQYLQGSDKELTVTGFKRIALG"
     gene            complement(6364..7266)
                     /gene="rpsB"
                     /locus_tag="VFU15_06605"
     CDS             complement(6364..7266)
                     /gene="rpsB"
                     /locus_tag="VFU15_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009016889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEU4717482.1"
                     /translation="MSRTSFNELLEAGAHFGHLKRKWNPAMAPYIFTEKNGIHLIDLN
                     KTVAKIDESCNILKGIARSGKKILFVATKKQAKDIVAEKVKSVGMPYVTERWPGGMLT
                     NFATIRKAVRKMAGIDKMATDGTFENLSKKEKLMLSRERAKLEHQLGSIADLTRLPAA
                     IFIVDIVKEHIAVAEAKRLGIPTFAIVDTNADPNLVDHSIPANDDASTSVSLIIGIVC
                     KAVAEGLEERKMDKDKAEEEREKEEAEEKISRFEKTIDEEEVASTVAGGAKRGKTSPA
                     VSKNTGGPSKGPNKGGGSRKPTRA"
     gene            complement(7306..7692)
                     /gene="rpsI"
                     /locus_tag="VFU15_06610"
     CDS             complement(7306..7692)
                     /gene="rpsI"
                     /locus_tag="VFU15_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012780290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEU4717483.1"
                     /translation="MEVTNTLGRRKTAVARVYLTRGKGDITVNGRPYKEYFATTVLQN
                     KVIQALAVHNLVDQYDVNVNVAGGGVNGQAEAIRLGIARALVENDAELKGKLRAEGLM
                     TRDPRMVERKKFGRRKARRRFQFSKR"
     gene            complement(7692..8156)
                     /gene="rplM"
                     /locus_tag="VFU15_06615"
     CDS             complement(7692..8156)
                     /gene="rplM"
                     /locus_tag="VFU15_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014202323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEU4717484.1"
                     /translation="MNTLSYKTVSANKATVKKSWLLVDAENEAVGRLASKVAYLVRGK
                     HKASYTPHVDCGDNIVIINAEKVRFSGSKMTDKVYKRYTGYPGGQRFATPKELLASAP
                     EKILEHAIGKMLPKNKLGDRLRRNVFIYAGTEHSQSAQNPVKIDLNTVKERK"
     gene            complement(8305..10254)
                     /locus_tag="VFU15_06620"
     CDS             complement(8305..10254)
                     /locus_tag="VFU15_06620"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU4717485.1"
                     /translation="MRKVFTLIVLFALMLPGVVRAQDKARLRQCVAALDTVREDTTRA
                     VMLNEIGWDTSYEDLAVGLTYAERSLELSKKIGFNHGMMLANNTIGTIYTDMGEYDKA
                     LQSLHDCLSLAEKDGNLYSQATSYLNISIVYKEMGDKQKALAAALESQKIYQKMELKK
                     GLCVVSNNVGSSYLDLDSVQKAIEFFSLSLRLSRELKNPDFESHALSGLSECFVETGD
                     SVTAEKYILRSIAIMDSLHDDYDRAQGIYDYGNLLAHYNRFPEAEAKYNEALQLFRKI
                     GMTDEEKSVWESFSELYEKEKDPVRALGALKKYMHLKDSLLNEKVLQHQHELETLYET
                     EKKQNQIDNLTKEQTIQDTYMAALVAGVVLLVIILLILFNRNKLRRRTNLQLEKQNAI
                     IEEKNKDITDSINYARKIQEAILPLPQGLNEQFSDAFVFYLPKDIVSGDFWWFSAGEE
                     EFILAVGDCTGHGVPGGFMSVMGASFLSEIINEKRLTAPDQILNTLRHKVVNALKHSN
                     VDAGEIRDGMDIVVCNFSKNNRMLSFAAANNPLWIARKGSVIEFPADKFPVGPHHDVV
                     RDFSLQQADLQPGDMIYLFTDGYADQFGGNDGKKFKYRRLKDLFASVSGLSCSEQHKV
                     LLSEFEKWKGSLEQVDDVLVVGLRV"
     gene            complement(10278..12269)
                     /locus_tag="VFU15_06625"
     CDS             complement(10278..12269)
                     /locus_tag="VFU15_06625"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU4717486.1"
                     /translation="MRSRRNFLFFFFLLLCAGSYAQERDQLRAAVKQLAEETVDTTRV
                     NLLNEIGWDTSYDNLSLGLKYCREALKLAEEIHYDKGQRVAYNYMGTIYQDMGELSNA
                     LDAHLKSLRIAEKNSNPGGMATSYMNLGLLYSSMHDPQQSLVNLKKAQQIFTEMKTDR
                     GMAAASDNLGAIYIELDSFAAAEREFSSGYALAVKIGKPVMQAHCLEGLSKCFQQKGD
                     TVAADAYMLRALHICDSLQDDYDLSQGMVSYADLLTERGRFDKAEIYLQKALAIDRRI
                     GMTDEEADIMREFADLYEKWNKKDLAILYLKKYYSEKDSISGEKVQRHQKELEAVYEN
                     DKKVSEIAHLTKEKTMQTVLLGAMGVGVVLLLAFLVILFNRNNLRRRTNEQLTAQNTI
                     IEQKNKDITDSIQYAQRIQSALLASGEMLRNNLGEHFVFYLPKDIVSGDFYWAAAGAN
                     APSSFYLAVCDCTGHGVPGAFMSLLNISFLNEAVKQNRLSRPAEIFDHVRKRLIENIS
                     GNGDPSSGRQDGMDGILAHFENNILTYAAAHNAPVLVRKGSITELPADKMPVGNSPKN
                     DVPFTSHEVKLEPGDVIYFFTDGFADQFGMSREAWALTAAPHLAGGKKFKHKNLRELL
                     GKISHLKMEEQEKELLNAFSEWKGPLEQVDDILVIGIRV"
     gene            complement(12291..14216)
                     /locus_tag="VFU15_06630"
     CDS             complement(12291..14216)
                     /locus_tag="VFU15_06630"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU4717487.1"
                     /translation="MYFSSEWLKQRLSSISDRQLQIEEQIIFMTSASDFLVEECYAIC
                     LELLEEGEKTGNPDLCGFALLGMSYYHRMHGNMEKGNALESRLENLLPEMKESFPTAI
                     VLQMLAFNYWSVGQRDRAFEYAYSSARMTEKIGGEGRGWSAFQFGVFYSDMKDYDLAL
                     ENFTKAEAQSAELGLVYQLARSRSGIAGVHLGRNRLEESLAYNRLALEGYRSCGHQTA
                     LSRALNDLGVITRKLGNNAEAETYLREALSIRELQKYVPGIITSQMELAHVLIANGDS
                     EESGELLGKALALAERTGSKQKIAECHRLLSELYKLKKEPWKALEHLDKFHEVRSGIA
                     GEEASNRIQHLQQKFATEKSRQEAEIHRLRNVELKKAYEEIEEKNRSITDSIHYAKRI
                     QRALLATDSFIARYVPEFFVLYKPKDIVSGDFYWAAAGTGNREQGAHLPDDANAPRSF
                     YIAACDCTGHGVPGAFMSLLSFSFLNEAINERKLSLPGNILGYVREKLVTSISDDGQR
                     DGMDCTLVSFDDKKKQVAYAAAYNAPVLVRNGEVKELSADKMPVGKSENATPFSTFTC
                     DVQEGDVLYLFTDGYADQFGGPRGKKLRHKNLLRILGEINHLPLAEQKSVLEERHAEW
                     KGDLEQLDDILIMGLRI"
     gene            complement(14251..16224)
                     /locus_tag="VFU15_06635"
     CDS             complement(14251..16224)
                     /locus_tag="VFU15_06635"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU4717488.1"
                     /translation="MKLRALLLAFLFPLFLCAGNGDTLETYLKSVPRDTAAVNKLMAI
                     SEKLSRTDEDAARRVAEEALAIAKETGDKPSVALAMLTLGVRYMNEGDYVKSSQFLFP
                     SMTTADSIGNNTIRLRVMNSLGNLYARQNQFKEAEGYYEKALALARAVHNRDKEGMIL
                     GNLGNIYYLQSEGDSTSLDKSIFYYQQALKISREDNAVYREINALNNLTLVYGDKKLY
                     AQALAVCDTLLALITAHRDSVDLVYLYVNYARIYRYQGDYANAIANFNRSLLLAQTLG
                     MKDMIAEDYHSLAICYSLTGDYKKAYEYQDKYTDLQNSLVNTENTQIISDLKNKIRTQ
                     EKENVIASLKQRNEVADLRNSRQNIYLGVSIAGVLIFIVLAFLFFTRARMKEEANRRL
                     GQQNDVIAQKNKEITSSIQYAQRIQQTLLASEDSLRSRLGEFFIFYRPKDIVSGDFYW
                     ATERVETGGNRAERSPFWLAVCDSTGHGVPGAFMSLLNISFLNEAISEKGMSAPGDVF
                     GHARKRLIESISQEGAQDGMDGTLFVFEGNRLRYAAAHNAPVVFRNGVMMELSCDKMP
                     VGKSPHEQTPFTTFETELRPGDVVYAFTDGYADQFGGHDGKKFKYKNLLKLLGDTCHL
                     PMAEQKKLLEKKYDEWKGALEQVDDVLVVGFRA"
     gene            complement(16365..17552)
                     /gene="kbl"
                     /locus_tag="VFU15_06640"
     CDS             complement(16365..17552)
                     /gene="kbl"
                     /locus_tag="VFU15_06640"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014202745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HEU4717489.1"
                     /translation="MYGEYKQFLESELRSIDEAGLFKRERVITTPQGAVVKVNGKDVI
                     IFCANNYLGLSSHPKVIEAAHRTLDTHGYGMSSVRFICGTQDIHKTLEEKIAKFHGTE
                     DSILYAACFDANGGVFEPLFGEQDAIISDELNHASIIDGVRLCKAQRFRYKNCDMNDL
                     EAQLKAAAGCRFRIIVTDGVFSMDGFVAPIDKICDLADKYNALVMVDESHATGFIGKT
                     GRGATEASGAMGRVDIITGTLGKALGGAMGGFTTGKKEIIEMLRQRSRPYLFSNSLAP
                     SIVGASIAVFDMLSETTALRDKLEANVNYFKAGMDHIGLEYRKGDSAIVPVMLYDAKL
                     AQQFAEKLLAEGIYVIGFFYPVVPKGQARIRVQLSAGHEREHLDKAITAFEKIGRELG
                     VIK"
     gene            17852..>19393
                     /locus_tag="VFU15_06645"
     CDS             17852..>19393
                     /locus_tag="VFU15_06645"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU4717490.1"
                     /translation="MKKLVAAGALFLCGILSGQKANIDSLVRALNAEKPDTSRVNTLN
                     ELSWQLKRSRPRTALAYGFDALKLAHSLGFKRGEGEACNNIGNTYDNLSVYDSALVFH
                     NKALALYTSIGYSKGIAGSYNNIGEIYRATGKYAEALESYFLSVDIKKELNDVKGLAN
                     SYNNIGSVYNAIGDYAKGLEYQLLSLSMKETLGDPAKIGSSCQNIGQLYHQLGNDSAA
                     LRYTDRSIALYTEANDSLSLSRACSNKGTILVSLGKYSEAMQNFSRATLIAQQLGVKR
                     ELLMVYNNIVNYYSVRSQFDSALVWSQKALGIGKTLGDSGAIGLIYYNYGEIWHHKGD
                     KAKAISYLKQARVCGLATEDARLIRESCHALAQLCEETGDYRNAMLYEAQYSELQEKE
                     FSDNTAKQLAIQQVKFDTEKKEKEIHVLKQKQVIDRLGISSRDLIIEKRNYFIAGLAA
                     TSLLLAFSILLFWSRRKIIIRNREEKAAREAGETERVRIAKDIHDDLGSGLTRIAILS
                     RQNEKE"
BASE COUNT         4213 a         5364 c         5090 g         4726 t
ORIGIN      
        1 gttccccgtg gggactttgc atcggtgact ataacaacga cggttataac gacgtattcc
       61 agggcagcag ctccaacggc tatctcctta cgtcatccaa cgccacgagc ttcacgctcc
      121 agaacatgac caccactttt ggtggcggaa gcgtgttcac acagggctgt aatttcgccg
      181 acatcgataa cgacggattc ctcgacctgt tcatctgcca tgataccggc aaaccgaaag
      241 tatacatggg cgacggaaca acgggcgggt ggacattcga ccagtccgtc atgccgcttt
      301 cgcagtaccc gatcctcata aattccgatt caaacagcgg gaactatgct tccatctgga
      361 cagacgtaaa caatgacggc tacatcgaca tgatgatcac gcactgccgc cagagcatca
      421 cgaattcgag tgatccgcgc cgcatcgacc agatcttcat caacaacgca aacggaacgt
      481 acacgcagga cacgtcgaac tggacaggtc tccgcgacgg agcgcaggga tggagcacgg
      541 cgtggggcga catcgacaac gacggtgaca tggatgcgtt cgtgctcaat tatgatgtga
      601 acagcaaagt aatgatcaat gacgggaacg gtcacttcac tgacgtgatg gccacctccg
      661 gcattgcgaa taccacgact tacttcggtg aaaatgcttc cttccacgat ttcgataatg
      721 acggtttcct tgacctgctc attaccggag accagcacta cctctaccag ggtcacggcg
      781 atggaacatt tacattggtc agcacccagc cttttgatta ctggaatgcc agccacacga
      841 ccttccggta catccgcggc accggcgttg gtgacctgaa cggagacggt ttcctcgacg
      901 tttacgccag ctacgccaat ctttacaatt ccgcgacgac cagcaagaac gaccacctct
      961 ggatgaacac gtgcgttggc aatggcaacc actggatcaa gttcaacctc gtgggcggtg
     1021 cagctcccgg gatgagcaac aagaatggcg tgggatcgat cgtgaaaatt tatggtccgt
     1081 ggggtgtgca ggtgcgcgaa gttcgttcag gtgaagctta cggactgcag aattcgctca
     1141 gcgtttattt cgggctcggg cagaacacgg cgatcgacag cgctgttgtc atctggcctt
     1201 ccggaatcgt ggacgagctg cttacgctta acggcgacaa ttcgtatacc gtcatggaag
     1261 gcggcaaccc gacctccaca cacccggttg agcagaaagc gctttctatg gcggtctacc
     1321 cgacacccgc aagcgattat gtgaacatcc gtctcgataa tttcgcgcag tacggactga
     1381 acaacctcag cctgaaaatt tatgacggca ccggcaagct cgtttattcc gaagccgcgc
     1441 tgcagacctg cattgtgacg atcggaaagg atattctttc cagcggcatt tacatggtcg
     1501 agatcgacaa caaggaaaaa cgcattgcca gcgacaagct gatcatgcaa taaattcagt
     1561 tgcacaccag gaaacgtccc gtgaatattt tttttacggg gcgttttttt acaaacacgt
     1621 tccaatacaa caacgacatg aaaaaattcg tactagctcc tgcgttcctg ctgccgtttt
     1681 tcgctgctgc gcagaatgtc atttcattct ccgaccagtc gacgctactc cccacgtcga
     1741 acttccacag cggaaactcc atcggtgtcg tcgacatgaa cggcgacaag aaggacgaca
     1801 ttgtacgcgc cagcaacaac gctacgatgt acatcgagta ccagaacgcg ccgaatgcaa
     1861 cattttccga gaacagcttc gcgaacagca tcggcgatcc gtggggcatg tgcgtgggcg
     1921 acctgaacaa tgacggctac aacgatgtct tctggggcga ttacgggaac acgtgggtgc
     1981 tggtacgcaa ttccgccacg acttacaccg gaacgaacat cgccgccacg ctgtacccgg
     2041 gattcatttt tgtacaggga tgcaatttcg ccgacatcaa caatgatcac tttctcgatc
     2101 tgttcgtgtg caacgacgat tactctccgc acatttacgt gaacaataac ggtacgggat
     2161 ggaccttcaa ccagggactc atgccgctcg ccacttttcc ctcatccgac ggaagcggca
     2221 attacgcttc catctggacc gacatcaaca acgacggcct gaccgattgc atggtcacgc
     2281 actgccgcca gggcgtaacg agttcttcgg acgcacgccg catcgaccag gtgtttctca
     2341 ataacgggaa cggaacgtac acgcaggaca tcaccaactg gaccggcctc cgcgacggtg
     2401 cgcagggatg gagcacggcc tggggcgaca tcgacaatga cggagacatg gatgcattcg
     2461 tgctgaacta cgacgtgaac agcacgctca tgatcaacga cggaaacggt gttttcacaa
     2521 acgtgatgag cggatccggc atcaacaaca cgacgacgat cttcggtgag aatgcaacgt
     2581 tccaggattt cgacaacgac ggctatctcg atcttatgat ctccggcgat catcacctcg
     2641 tttaccacaa caatggaaat cacacgttca cgctgcaggc aacggatccg ttcccgtacg
     2701 caggccacac gatcaccgcg cacgctgtcg gcgacctcaa cggcgacggc tggctcgacg
     2761 tgtacgccag ctactgcgac atataccagt cggccaacag ctcccgcaac gacaagctgt
     2821 ggatgaacaa cacgaacaac ggcaatcact acgtgatgtt cgatctcgtc ggcggtgcga
     2881 tcaacgggtt ttcaaacaag aacggcgtcg gtgcgagcgt gaaaatttac gggccgtggg
     2941 gcgtgcaggt gcgtgaagtg cgttccggcg aagcttacgg catccagaat tccatgtgct
     3001 gccacttcgg tctcggatcg aacacgaccg tcgacagcgt cgtggtgcgc tggccttccg
     3061 gcatcgtcga cgtgatgacg aaccttccct gcgaccagca ttacacgatc aacgaaggca
     3121 tgtcgcccac ggcgacggcg tatctctccg aaaagccggt ttcacttgcc gtttacccga
     3181 atccggtaag cgatgtcgca tcgatccgcg tgcagaattt cgcgcagtac gggctcaaca
     3241 atctttcgct gaatatttac gacgtcaacg ggaagctcgt ttattccgaa gcggaactgc
     3301 agcagagcat catcatgatc ggccgcgacg tgctgagcag cggactttat ttcgtcgagc
     3361 tgaaaaataa ggacaagcga ctggcgagcg agaggatgat ggtggaataa gagctccgat
     3421 cgtttttgcg gaaatttcga gagtggtgcc tgaagttgat ttcgggcacc atttttttac
     3481 aggttttttg cttcgtcgcg ggattgatca accggtaatt ttcacttcac ctgccgcctg
     3541 caaaaaacgg gcttgccgaa taaaaaaagc gccggagaat tccagcgccc tttcgttatt
     3601 cttttctgac gttcacaccg tcatgatctc tttctctttc gcttcaagat gcttgtccac
     3661 tttcgcgatg aaattatcgg tgatcgtctg cactttcgct tcgccctctt ttgcttcatc
     3721 ctcggggagt ccgttcttct ggagcttttt cacctcttcg ttagcgtcgc gtcgcgcggt
     3781 gcggatgctc accttcgcgt gctcgccttc ctctttcgct tttttcacga gcgctttgcg
     3841 ccgctcttcc gtcatcgggg gaatggcgca gatgataagc gttccgttgt tggtgggatt
     3901 gaaaccgaga tttgcggcct gtatggcttt cagcacggga tcgagcattc ctttttccca
     3961 gggctggacg gagatcgtac gcgcatcggg cgtgttcacg ttggccacct ggctgagcgg
     4021 agtattcacc ccgtaataat ccacgtgtat accgtccagc attccagcat tggcgcggcc
     4081 tccgcggact ttcactaatt ccgcctcgag gtgtttcacg gcatggtcca tcgcttcctg
     4141 cgcggtttct atgataagct gaagatcttc gttcatatct gtttgcgttt aatggataaa
     4201 aatagcaaaa gtttgcggcg taaagaccgg ccgggctggc gctcaaaatg tatatttggg
     4261 aaacaaactc aaatccatac accaatgaaa ttcatcaaat cttttgccgc gatcgttatc
     4321 gcattcgttc ttgtcgcagg agcttcttcc tgcaaaggtg gctctgcttt caaagtcgga
     4381 gatcatgttg ccgccaaatg gaccaataat aattactacc tcggatcgat cacggccgtt
     4441 aacgccgaca agtattcgat cctttacgac gacggcgaca agctcgacgt tccggttacg
     4501 gacctcaaaa cgctctgcaa ccagtcggat ctcaaagtcg gcgataaggt aatggcctcc
     4561 tggaattcca gcgtcatgct ttatcccgga accgttaccg agctgcaaag cggcggtgcc
     4621 atggtaaagt gggacgacgg cagcgaacct tccctttctc cttacgggaa gctgacaaaa
     4681 taattcctgt tccgttacta atgaaaaatc cgccggtggc ggattttttt atgcgtgcat
     4741 aatttcagaa tgcgacgagg gttcccactt tttcgcccgt aacgacgcgg gaaagattcc
     4801 ccggtcggtt catgtcgaaa acaatgatgg ggagcttgtt ctcgttgcag agcgtgaacg
     4861 ccgtcatgtc cattacgttc agtccttttt catagacttc attgaacgtg atcgtttcga
     4921 atttcgtcgc cgttttatct ttttccggat ctgccgtgta gatgccgtcg acgcgcgttc
     4981 ccttgaggac tacatcggct tctatctcga tagcgcgcaa tgaagcggcc gtatcggtgg
     5041 tgaaataagg atttcctgtt cccgatccga aaataacgac acggttcttt tcaagatgcc
     5101 gcacagcacg gcggcggatg aacggttcac agatctgctt catttcgatc gcgctctgga
     5161 gccgcgtcgg tatctcgagt ttttcaagcg ccgcctgcag cgccatgctg ttgatcatcg
     5221 tggcgagcat gcccatgtaa tcgccctgca cgcggtccat tcctccgtca gccgcctgga
     5281 tgccgcggaa aatatttcct ccgccgatca cgatcgctac ttccacaccg gccgccacta
     5341 cgcttttgat ctcctgcgcg tactggttga ggatctcatg atcgatgccg aattgctttt
     5401 cgcccatcag ggcttcgccc gacagcttga gaagaatgcg tttgtatttc ataggccttg
     5461 gattggcagg tcaaaaaaaa tcccccgaaa aacgggggaa tgaaaattga attatccgag
     5521 agcgattcgt ttgaagcccg ttaccgtgag ctctttgtcg gagccctgca ggtactggcg
     5581 aatcgatttc ttgctgtcct tgatgaactc ctggttgagc agcgtggact ctttgtaaaa
     5641 cttgttcagc ttgccctgcg cgatcttctc gaccatatct tcggccttgc cttctgcacg
     5701 cacctgctcg cgggcgatat caagttcgcg ttcgagcgtc gtggtgtcca catcgtcttt
     5761 gtcgacagca acgggagcca tcgcggccac ctgcatggcg acgtctttcg ccatatcgtc
     5821 ggaagcttct ttgttgaagc caacgatgca ggccagctta ttgccggggt gattgtacgc
     5881 caccactttc gcagcggaga tctgctcgta acgggaaagc tcgatcttct cgccgatctt
     5941 tccgacgagg tcgatcactt tttcaccgat agtcgtgctt tcaaaaggaa gcgccagaag
     6001 cgcagcgaga tcagccggcg ctttgtcgag ggcgatctgc gcaaacgtgt cagccacttt
     6061 cgcaaaatca tcgttcttcg ccacgaaatc cgtttcgcag ttcagcacga tcacaacacc
     6121 gcggccgccg ttcgttttgg ctaccacctg tccttctttc gcatcacggt cgctgcggtt
     6181 ggcagccact ttctgtccct ttttacggag aagatcgata gccgcgtcga aatcgccgtt
     6241 ggtttcggtg agcgcttttt tgcaatccat catacctgcg cccgtcatgg tgcgcagctt
     6301 atttacgtct gctgcagtga tagtagccgt catgttcaaa atgctttaga aataaaaact
     6361 gtattacgcg cgggtcggtt tgcgcgatcc accgcctttg ttggggcctt tggaaggacc
     6421 gcctgtgttc ttcgaaactg cggggctggt ttttccgcgc ttggcgccgc cagccactgt
     6481 cgatgcgact tcttcttcgt cgatcgtttt ctcgaagcgg gagatcttct cttcggcttc
     6541 ttccttttcg cgttcctctt cggctttgtc cttgtccatt ttgcgctctt caagaccttc
     6601 agcgactgcc ttgcagacga tgccgatgat gaggctgaca gaagtggaag cgtcgtcgtt
     6661 ggccggaatg gaatggtcaa cgaggttcgg atcagcgttg gtatcgacga tagcgaacgt
     6721 cgggatgccg aggcgtttcg cttccgccac cgcgatgtgc tcttttacga tatccacgat
     6781 gaaaatagca gcgggaaggc gggtaaggtc ggcgatggaa ccgagctggt gctcgagctt
     6841 ggcgcgttcg cggctgagca tgagcttttc tttcttcgaa aggttttcaa acgtgccgtc
     6901 ggtagccatt ttgtcgatac cggccatctt gcgcaccgcc ttgcggatgg tggcgaagtt
     6961 ggtcagcatt ccgccgggcc agcgttccgt tacgtaaggc attcccacgc ttttcacttt
     7021 ttcggcaacg atgtctttcg cctgcttttt tgttgcgacg aaaaggatct ttttgccgga
     7081 acgtgcaatc cctttgagga tattgcatga ttcgtcaatc ttcgcgaccg tcttgttgag
     7141 atcgatcagg tggatgccgt tcttctccgt aaaaatatac ggagccatag cgggattcca
     7201 tttacgcttg aggtgaccaa agtgtgcacc tgcttcgagc agctcgttga aagaagttct
     7261 tgacatgtac tttcgactgg atattgtgat aatagatcgt ttgaattagc gcttgctgaa
     7321 ctggaagcgg cggcgggctt tgcggcgacc gaactttttg cgttccacca tgcggggatc
     7381 gcgggtcatg aggccttctg cacgcagttt tcctttcagc tccgcatcgt tctcaaccag
     7441 ggcgcgtgcg atgccgagac ggatcgcttc tgcctggccg ttcacgccgc caccggcgac
     7501 gttaacgttc acgtcgtact ggtcgacgag attgtgaacc gccagggcct ggatgacttt
     7561 gttctggaga acggtcgttg caaaatattc tttgtaagga cggccgttaa cggtgatatc
     7621 acctttaccg cgtgtgaggt aaacacgtgc gactgcggtc ttgcgccttc cgagagtgtt
     7681 ggttacttcc attacttgcg ctctttaact gtattgagat cgatttttac aggattctgc
     7741 gcggactggc tgtgttccgt tccggcataa atgaaaacgt ttctgcgaag acggtcgccg
     7801 agcttgtttt tcggaagcat tttcccgatc gcgtgctcga ggatcttttc aggtgcgctc
     7861 gcaagaagtt ctttcggcgt agcgaaacgc tgtccgccgg gataacccgt gtaacgctta
     7921 taaaccttgt ccgtcatttt ggaaccggaa aagcgaactt tctcggcatt gatgataacg
     7981 atgttatctc cgcaatcgac gtgaggcgta taagaagcct tgtgcttgcc tctcacgagg
     8041 taagcaactt tggaagcaag acgtcccacc gcttcattct cggcatcgac cagcaaccat
     8101 gattttttta ccgtggcctt attggcggaa acggttttgt aggatagcgt attcacttga
     8161 ttttcagttg aataatactt cttcgtgcgt tctgtaccct taaatggggc tgcgaagata
     8221 agtagaaaat ctctcccgcc aaattttata aacaaataat atgttgaaaa cgggaccgtc
     8281 ccgggggccg gacaggccgt attatcacac tctcaggccg acaacgagaa catcgtcgac
     8341 ctgctcgagg gaacctttcc acttttcgaa ctcggaaagg agcactttat gctgctcact
     8401 gcagctgaga ccgctgacgg aggcgaataa gtccttgagg cggcggtact tgaacttctt
     8461 cccgtcattt ccgccgaact ggtcggcgta accgtcggtg aacaggtaga tcatatcccc
     8521 gggctgaagg tcggcctgct gcagcgaaaa atcgcggacc acatcgtggt gcggtcctac
     8581 gggaaatttg tcggcaggga actcgatgac agaacctttc cgggcgatcc agagcgggtt
     8641 gttggcggcg gcgaaggaaa gcatgcggtt gttcttcgaa aaattgcaga cgacgatatc
     8701 catcccatcc ctgatctcgc ccgcatcgac attcgagtgc ttgagcgcgt tgacgacctt
     8761 atggcggagc gtattgagta tctggtcggg cgcggtgagc cgtttttcgt tgatgatctc
     8821 ggaaagaaag gaagcgccca tgacgctcat gaacccgccc ggaacgccgt ggcccgtaca
     8881 atcgccgacg gcgagaatga attcttcttc acccgcgctg aaccaccaga aatcaccgct
     8941 gacaatatcc ttggggaggt aaaagacgaa cgcatcggag aattgctcat tcagtccctg
     9001 gggcaaaggc agaatggctt cctggatctt gcgcgcataa ttgatcgaat cggtgatgtc
     9061 cttgttcttt tcctcgatga tggcgttctg cttttccagc tggaggttcg tccggcggcg
     9121 gagcttgttg cggttaaaaa gaatgagcag gataataacg agcagcacca caccggcaac
     9181 cagcgcggcc atgtaagtat cctgtatcgt ctgctccttg gtgaggttgt cgatctggtt
     9241 ctgctttttt tccgtttcgt ataatgtctc cagctcgtgc tggtgctgca gcactttttc
     9301 attcagcagc gaatctttca ggtgcatgta ttttttcagc gcaccgaggg cacggacagg
     9361 atctttttcc ttttcataca attccgaaaa actttcccac acgcttttct cctcgtcggt
     9421 catgccgatc ttgcggaaga gctgcaatgc ctcgttgtat tttgcttcgg cctcggggaa
     9481 ccggttgtaa tgcgccagca ggttcccgta gtcataaatt ccctgggccc ggtcgtaatc
     9541 atcgtgcagg gaatccatga tcgcgatgct gcgcaggatg tatttttccg cggtgacgga
     9601 atcgccggtt tccacgaaac attcggacaa cccgctgagt gcatgcgatt cgaaatcggg
     9661 atttttaagc tcgcgtgaaa gacgcaggga gagggagaaa aattcgatcg ctttctgcac
     9721 gctgtcgaga tcgagatagg aagagccgac gttattgctg acgacgcaga gtcctttctt
     9781 cagctccatc ttctggtaga tcttctgcga ttcgagcgcg gccgcaaggg ctttctgctt
     9841 atcgcccatt tccttgtaca cgatggaaat gttgagatag gaagtcgcct ggctgtacag
     9901 gttgccgtcc ttctcggcga gcgaaagaca atcgtgcagc gactgcagcg ccttgtcgta
     9961 ttcgcccata tcggtgtaga tggtaccgat cgtgttattc gccagcatca tcccgtgatt
    10021 gaaaccaatc ttcttcgaaa gctcgagtga acgttccgcg taggtgagcc cgacagcaag
    10081 atcttcatag ctggtgtccc agccgatctc gttcagcatt acggcgcgcg tcgtgtcttc
    10141 gcgcaccgtg tcgagcgccg cgacgcactg gcggaggcgc gctttgtcct gcgcacggac
    10201 gacacccggg agcataagcg caaacaaaac gatcagcgtg aaaaccttcc gcatccgcca
    10261 aaagtagcaa agcagactca gacccttatt ccgatcacga gaatatcatc cacctgctcg
    10321 agcggacctt tccattcgct aaaagcgttt agcaattcct tttcctgctc ttccattttc
    10381 agatgtgaaa tttttccgag cagttcacgg agatttttgt gcttgaattt ttttccgccc
    10441 gcgagatggg gcgccgccgt caacgcccat gcttcacggc tcatcccgaa ctggtcggcg
    10501 aagccgtcgg tgaaaaaata aatgacgtca cccggttcga gctttacttc atgggaagtg
    10561 aacggaacgt cgttcttcgg tgaattcccc acgggcattt tatcggccgg caattcggtg
    10621 atgcttcctt ttcgcacgag cacgggcgca ttgtgcgcag cggcgtatgt aaggatatta
    10681 ttttcaaaat gcgcgagaat gccgtccatt ccgtcctgcc tgcccgaaga aggatctccg
    10741 ttgccggaaa tattttcaat gagccgtttc cgcacgtgat cgaaaatttc tgccggacgg
    10801 gaaagacgat tctgtttcac ggcttcattc aggaaagaaa tgttcagcag cgacatgaac
    10861 gctcccggaa cgccgtgccc ggtacagtcg cagacagcga ggtaaaaaga ggacggcgca
    10921 ttcgcaccgg ctgccgccca atagaaatcg ccgctgacaa tatccttcgg aaggtaaaac
    10981 acaaaatgct cgccgagatt gttgcgcagc atttcccccg aagcgagcaa ggccgactgg
    11041 atacgctgtg cgtactggat ggaatccgtg atgtccttgt tcttctgttc gatgatcgtg
    11101 ttctgcgcag tgagctgctc attggtccgc cggcggaggt tgttgcggtt gaaaagaatg
    11161 acgagaaacg cgagaagcag caccacgccg acgcccattg cgccgaggag caccgtctgc
    11221 atggtttttt ccttcgtcag gtgtgcgatc tcgcttactt ttttgtcgtt ctcatacacc
    11281 gcctcgagct ctttctggtg gcgctgcact ttttctccgc tgatgctgtc cttttccgaa
    11341 taatattttt tcaggtaaag gatcgccagg tcctttttat tccatttttc gtagaggtct
    11401 gcgaactcgc gcatgatgtc cgcttcttca tccgtcatcc cgatcctgcg gtcaatcgcg
    11461 agcgctttct gaaggtagat ctccgcttta tcgaaacgtc cgcgttccgt gagaagatcc
    11521 gcgtagctca ccattccctg ggagagatca taatcatcct gcagcgaatc gcagatgtgc
    11581 agggcgcgca gcatatacgc atctgccgca accgtatcac ctttctgctg gaagcatttg
    11641 gaaagtcctt cgaggcaatg cgcctgcatc accggcttgc cgatcttcac ggcgagcgca
    11701 tacccggaag aaaattcccg ctcggccgca gcgaagctgt ccagttcgat gtaaatggcg
    11761 ccaagattgt cgctggcagc cgccatgcca cggtctgttt tcatctccgt gaaaatctgc
    11821 tgcgctttct tcaggttgac aagcgactgc tgcggatcgt gcatggagga atacaacagg
    11881 ccgaggttca tgtatgacgt cgccattcct cccgggttgg aatttttttc ggcaatgcgc
    11941 aggcttttca ggtgagcgtc gagggcattg ctgagctcgc ccatatcctg gtaaatggtg
    12001 cccatgtaat tgtaggccac acgctgccct ttgtcgtaat gaatttcttc ggcgagcttc
    12061 aatgcttccc ggcagtattt aagtcccagc gaaagattgt cgtagctcgt gtcccacccg
    12121 atctcattga gcaggttgac gcgcgtcgta tcaaccgttt cttccgccag ctgttttacg
    12181 gccgcgcgca gctggtcacg ttcctgcgcg tacgatcccg cacacagcag caggaaaaag
    12241 aaaaacagga aattccgtcg ggaccgcatc gccgtttcag ctgtagtttg tcagatacgt
    12301 aaacccatta tgagaatatc atccagctgc tcgagatcac ctttccattc cgcatgccgt
    12361 tcttccaata cacttttctg ttccgccaaa ggcaggtgat ttatttcccc gaggatacgg
    12421 agcaggtttt tgtgccgcag ctttttcccg cggggaccac cgaactggtc ggcgtagccg
    12481 tcggtgaaaa gataaagcac atcgccttcc tgcacatcgc aggtgaacgt ggagaacggc
    12541 gtcgcatttt ccgattttcc caccggcatt ttatccgcgc ttagctcttt tacttcaccg
    12601 ttacgcacca gcacaggcgc attgtacgcc gcagcgtacg caacctgctt ctttttatcg
    12661 tcgaaagaaa ccagcgtaca gtccatgccg tcccgttgcc cgtcatcgct gatggacgta
    12721 acgagctttt cccggacgta accgaggata ttcccgggaa gcgaaagctt ccgctcgtta
    12781 attgcttcgt tgagaaaaga aaagctgagc agcgacatga aagcgcccgg aacgccgtga
    12841 ccggtgcagt cgcaggcggc gatgtaaaaa gagcggggcg cgttcgcgtc atccggaaga
    12901 tgtgcgcctt gttccctgtt ccccgttccc gctgccgccc agtagaaatc gccgctgacg
    12961 atatccttcg gtttgtaaag cacaaaaaat tccgggacgt aacgcgcgat gaaactgtcc
    13021 gtcgccagca gtgcgcgctg gatgcgtttc gcataatgaa tactgtcagt gatgctcctg
    13081 ttcttctcct cgatctcttc gtacgctttt ttgagctcga cattcctgag ccggtggatc
    13141 tccgcttcct gcctgctttt ctcggttgcg aatttctgct ggagatgctg tatcctgttc
    13201 gaggcttcct cgccggctat gccggatctt acttcatgaa atttatcgag gtgctccagc
    13261 gctttccacg gttctttttt cagcttgtac aattccgaaa ggagacgatg gcattccgcg
    13321 attttctgtt tcgagccggt gcgttcggca agcgccagcg cttttcccag cagctctccc
    13381 gattcttcgc tgtcgccatt cgcgatcagc acgtgggcga gctccatctg cgaagtgatg
    13441 atgccgggaa cgtatttctg cagctcacga atggaaagcg cttcgcgcag gtacgtttcc
    13501 gcttcggcgt tgttcccgag cttccgcgtg atcacgccga gatcgttgag cgcgcggctg
    13561 agcgcggtct ggtgcccgca ggagcggtag ccttcgagcg cgagccggtt ataggcaagg
    13621 ctttcttcca ggcggttcct gccgagatga acacctgcta ttcccgatcg cgagcgtgcc
    13681 agctggtaaa cgagaccgag ctccgctgat tgtgcttccg ctttggtaaa attctcgagc
    13741 gcgagatcgt aatccttcat gtcggaataa aaaacgccga actggaatgc cgaccatcct
    13801 cttccctctc cgccgatctt ttccgtcatc cgcgcgctgc tgtaggcata ttcaaacgcc
    13861 cggtcccgct gccccaccga ccagtaattg aacgcgagca tctgcaggac aatggccgtg
    13921 ggaaatgatt ccttcatttc cggcaaaaga ttttccagcc tgctctcgag cgcatttcct
    13981 ttctccatat tgccgtgcat gcggtgataa tacgacatcc cgagaagggc gaacccgcag
    14041 agatcggggt tcccggtttt ttccccttct tcgagcagtt ccaggcagat cgcgtagcat
    14101 tcttcgacga gaaaatcgct ggcgctcgtc atgaaaatga tctgctcctc gatctgcagc
    14161 tgccggtcgg aaatggaaga gagccgttgc ttcagccact cggaagaaaa atacattcgg
    14221 gaaggatttg atcaaaaata ggaaataacg tcacgcccgg aaacccacca ccagcacgtc
    14281 atccacctgc tcgagcgcgc ctttccattc atcgtatttt ttctccagca acttcttctg
    14341 ctctgccatc gggaggtgac aagtatcacc gagaagcttc agcaggtttt tgtatttgaa
    14401 ttttttcccg tcatgcccgc cgaactggtc ggcatagccg tccgtaaaag catacacaac
    14461 atcgcccggc cgcagctccg tttcaaacgt ggtgaacggc gtttgctcat gcggagattt
    14521 tcccaccggc atcttgtcgc acgacaattc catcatcaca ccgttcctga aaacaaccgg
    14581 ggcattatgc gccgccgcgt aacgcagcct gttcccttcg aacacaaaca gcgtaccgtc
    14641 catgccgtcc tgcgcacctt cctgcgaaat gctttcgatg aggcgtttcc gtgcgtgacc
    14701 gaaaacatca ccgggagcgg acattccttt ttcgctgatg gcttcattga gaaaagaaat
    14761 attcagcagc gacatgaacg cgcccggaac gccgtgtccc gtggagtcac atacggcgag
    14821 ccagaaagga gatctctccg ctctgttgcc ccctgtttcc actctttcgg ttgcccagta
    14881 aaaatcgccg gagacgatgt ccttcgggcg atagaaaatg aagaattccc cgagccggct
    14941 gcggagggaa tcttccgaag cgagaagcgt ttgctggatg cgctgcgcgt actggataga
    15001 agacgtgatc tccttgttct tctgcgcgat gacatcattc tgctggccga gcctgcggtt
    15061 ggcttcctcc ttcatgcgcg cacgtgtgaa gaaaagaaac gcaagcacga tgaagatcag
    15121 tacaccggca atggaaacac cgaggtaaat gttctgccgg ctgttgcgca ggtcagccac
    15181 ttcgttgcgc tgtttcagcg aagcgatcac gttttccttt tcctgcgtgc gtatcttgtt
    15241 tttcaggtcg gagatgatct gcgtattctc cgtattgaca agggaattct ggagatccgt
    15301 gtatttgtcc tggtattcgt acgctttttt gtaatcgccg gtgaggctgt agcagatggc
    15361 gaggctgtgg taatcttccg cgatcatgtc cttcatgccg agcgtttgcg ccagcagaag
    15421 cgaacggttg aaattggcga tggcatttgc ataatcaccc tggtagcggt agatccgtgc
    15481 gtaattgacg tagagataca cgagatcgac gctgtccctg tgcgctgtta tgagcgccag
    15541 cagcgtatcg cagaccgcga gcgcctgcgc gtacagcttt ttgtcgccgt acaccagcgt
    15601 aaggttgttc agcgcattga tctcgcggta aaccgcgttg tcctcccggg aaattttcaa
    15661 cgcctgctgg taatagaaaa tggatttgtc cagcgaggtg ctgtcgcctt cgctctgcag
    15721 gtaatagatg ttgccgaggt tgccgaggat catcccttcc ttgtcgcggt tgtgaacagc
    15781 ccgcgccagc gcgagcgctt tctcataata accttccgct tctttgaact ggttctgccg
    15841 cgcgtacagg ttcccgaggc tgttcatcac acgcaggcgg atcgtattgt tgccgatgct
    15901 gtcggccgtg gtcatggaag gaaaaagaaa ctgcgaggat ttcacgtaat cgccttcgtt
    15961 catgtaacga acaccgagcg tcagcatggc gagcgccacc gaaggtttat cacccgtttc
    16021 tttcgcgatg gccagcgctt cttccgcgac gcgcctggcg gcatcctcgt ccgtgcgcga
    16081 caacttttcg gaaatcgcca tcagcttatt gacagctgcc gtatcccggg gaaccgattt
    16141 caggtaagtt tccagcgtat cgccgtttcc cgcgcagaga aacaaaggga aaagaaaagc
    16201 gagcagcagg gcgcgaagtt tcatggccgg atgctcaaat gtagaaaatg tacaggtgag
    16261 aagtgagcag tgagacgccg gaacgtgtct caaaagacca cacagcgtct tcccgttccc
    16321 gggacggctc cggtgattcc agcccgcttg atgtttgctg ccggctattt gatcacaccc
    16381 agttccctgc ctatcttctc aaatgcggtg atcgctttgt cgagatgttc tctttcgtga
    16441 cctgcgctca gctgcacacg tatgcgcgcc tggcctttgg gaacgacggg atagaaaaag
    16501 ccgatgacgt aaattccttc cgcgagcagc ttttccgcga attgctgcgc aagttttgca
    16561 tcgtacagca tgacgggaac gatcgccgaa tcgcccttcc tgtattcgag accgatgtgg
    16621 tccatgccgg ctttgaaata gttcacgttc gcttcgagct tgtcgcgcaa agcggtggtt
    16681 tcgctcagca tgtcgaacac ggcgatggac gcaccgacga tggaaggcgc aagcgaattg
    16741 gaaaacaaat acgggcgtga acgctggcgc agcatctcga tgatctcttt tttccccgtg
    16801 gtgaatccgc ccatcgcgcc gccgagcgct ttgccgagcg tgccggtgat gatatcgact
    16861 cttcccatcg ccccgctcgc ttccgttgcg ccacgccccg ttttgccgat gaacccggtg
    16921 gcgtgcgatt catccaccat cacgagcgcg ttgtatttgt cggcgagatc gcagatctta
    16981 tcaatgggcg cgacgaagcc gtccatggaa aaaacgccgt cggtgacgat gatcctgaag
    17041 cggcaacccg ccgcggcttt gagctgcgcc tcgaggtcgt tcatgtcgca gttcttataa
    17101 cggaaacgct gggccttgca caggcgcacg ccgtcgatga tggacgcatg attgagctcg
    17161 tcggaaatga tggcatcctg ctcaccgaag agaggctcga acacgccgcc gttcgcatcg
    17221 aagcaggctg cgtaaagaat gctgtcctcc gtgccatgga atttcgcgat cttttcttcg
    17281 agcgttttgt ggatatcctg cgtaccgcag ataaagcgca cgctggacat tccgtagcca
    17341 tgcgtgtcaa gcgtcctgtg cgccgcttcg atcactttcg ggtgcgagga aagtccgagg
    17401 taattattcg cgcagaaaat gatcacgtct tttccgttca ctttcaccac ggcgccctgc
    17461 ggcgtagtga tcacgcgttc acgcttgaaa agtcctgctt catcaatgga gcgaagctcc
    17521 gattcgagaa attgtttgta ttcgccgtac ataaatgttc aaggttcgat gttcaacttc
    17581 cgtgctttcc gcccttgcgg agaggaagat caaagttaat ttttattgaa aagcggatac
    17641 cgctgctgtt cacggattcc gggagcggac ctgcattact tcttattccc ttcgttccgg
    17701 ctgtcgctat tatccgctgc gcgggataaa gcgcttccat ccgggctatc cgttccctcc
    17761 ttcctgttca tttaaaattc ttttccaccg ccgggttgtt ttctttgcca ttccccgcca
    17821 attcccgatt ttcacaaaca taatcgcggt tatgaaaaag ctcgttgcgg caggcgccct
    17881 gttcctctgc ggaatccttt ccggccagaa ggcgaacatc gacagcctgg tccgtgcgct
    17941 caacgcggaa aagcccgaca cgtcgcgggt gaacacgctc aacgagcttt cctggcagct
    18001 gaaacgcagc cgcccgcgca ccgcgctcgc atacgggttc gacgcgctta agctcgcaca
    18061 ttcgctcggc ttcaaacgcg gcgagggaga agcctgcaac aacatcggca acacgtacga
    18121 caacctgagc gtgtatgatt ccgcgcttgt cttccacaac aaagcgctcg ctctttacac
    18181 gtcgatcggg tacagcaaag gaatcgcggg cagctacaac aacatcgggg agatttaccg
    18241 cgccacggga aaatatgcgg aagcgctcga atcgtatttt ctttccgtcg acatcaaaaa
    18301 ggagctgaac gacgtaaaag gtcttgcgaa ttcctacaac aacatcggca gcgtgtacaa
    18361 cgcgatcggc gattacgcca aaggcctcga gtaccagctg ctttcgcttt ccatgaaaga
    18421 aaccctcggc gatccggcaa agatcggctc gtcttgccag aacatcgggc agctgtatca
    18481 ccagcttggc aatgattcgg cagcgctccg ctacaccgac cgcagcatcg cgctgtacac
    18541 ggaagccaat gattcgctca gcctttcacg cgcctgttcc aacaaaggaa cgatcctcgt
    18601 ttcgctggga aaatacagcg aagcgatgca gaatttttca cgggccaccc tcattgcgca
    18661 acagctcggc gtgaaacgcg agctgctgat ggtgtacaat aacattgtga attattacag
    18721 cgtgcggtcg cagttcgatt ccgctctcgt gtggtcgcag aaggcgctcg gcatcgggaa
    18781 aacgctcggc gattccgggg cgatagggct tatctattac aattacggcg agatctggca
    18841 tcacaagggc gacaaggcga aagcgatctc gtacctgaag caggcccgcg tctgcggact
    18901 tgccacggaa gacgcgcggc tcatccgtga atcgtgccac gcgctcgcac agctctgcga
    18961 agagaccggc gattaccgga acgccatgct gtacgaagcg cagtattccg aattgcagga
    19021 aaaagaattc agcgacaaca cggcaaagca gctcgccata cagcaggtga agttcgacac
    19081 tgaaaaaaaa gagaaagaga tccacgtgct gaagcagaaa caggtgatcg accgcctcgg
    19141 catttcgtca cgcgatctta tcattgaaaa gcggaactat ttcatcgcgg gactcgccgc
    19201 cacgtcgctc ctgcttgctt tttccatcct gctgttctgg tcgcggcgga agatcattat
    19261 ccgtaaccgc gaggaaaaag ccgcgcggga agccggcgag accgaacgcg tacgcatcgc
    19321 gaaagacatc cacgacgacc tcggttccgg gctcacgcgc atcgccattc tttcccggca
    19381 gaacgaaaaa gaa
//
LOCUS       DASTSF010000227         9214 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_481707,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000227 DASTSF010000000
VERSION     DASTSF010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 9214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9214
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_481707"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            385..1884
                     /locus_tag="VFU15_06650"
     CDS             385..1884
                     /locus_tag="VFU15_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007192148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEU4717491.1"
                     /translation="MNPLKKLAGQTAVYGMSTIFGRLLTYALTPFYTYIFGHPADFGI
                     NTEFYAYMSFLNVFFTYGMETGLFNFSVSEQNKGLVYSTALRSLFVSTVLFTLPVILF
                     AGPIADVLRYPGHGEFVIWVILIVATDALTAIPFAKLREQNKAKRFATVKLVNIAVNL
                     LINFFLIGYCKWAYEHHPESFWGSFYNPAIGIGYAFVAQLIANCVSVLLLLPELVQVR
                     SGFNWPLWKRMMGYSMPLLVVGLAGMTNETMDRILLKYLLPADISEAQVGIYGACYKI
                     SILMTIFIQAFKYAAEPFFFAQQKEQDAHILYGRVLKYFLLFCLLIFLGTTLNLSWIQ
                     YFVGKRYRVGIPVVPILLFANLCLGMYFNLSIWYKLTGRTRFGMWLTLFGALITLVLN
                     FAWIPSQGYFHSYMGSAWATLICYAVMMVLSYIFGQRYYHVKYDLPRLLMYTGLALAL
                     FFAGYYFISTGKAATDLLLRNLLFIVFILVIFIVEKPGRLLRRTSRPTG"
     gene            1907..2341
                     /gene="dut"
                     /locus_tag="VFU15_06655"
     CDS             1907..2341
                     /gene="dut"
                     /locus_tag="VFU15_06655"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010956168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEU4717492.1"
                     /translation="MKIKVINQSGHPLPEYATAHAAGLDLRANLEEPVSLRPLERAVI
                     PTGLFMEIPEGYEAQVRPRSGLAAKHGISVLNSPGTIDADYRGEIKVILVNLSNSAFT
                     VENGERIAQIVFAKHEQVEWISSVELTTSERGSGGFGHTGTK"
     gene            2435..3451
                     /locus_tag="VFU15_06660"
     CDS             2435..3451
                     /locus_tag="VFU15_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019946299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEU4717493.1"
                     /translation="MKIIIPMAGMGKRMRPHTLTVPKPLIPVAGKPIVERLVEDIAQV
                     CSEPLEEIAFIIGPSFGKEVEAMLVNVAGRLGAKGSIWYQEEALGTAHAIMCAAPVLD
                     GKVVVAFADTLFKADFRLDSSKEGIIWVQKIDDPRAFGVVKVNAENVITDFVEKPQTF
                     VSDLAIIGIYYFRDGANLRSELQYLLDNNIRDKGEFQLTNALENMKSKGVKFSPGKVT
                     EWLDCGNKDATVYTNQRMLEFKKDEEALVSSSVRSDNSEIIPPCYIGENVSLINTTVG
                     PHASIGSNCTLVNSFVSNSIVQPDTKITDATIRNSMIGKAAEITGGGETLDVSISDYS
                     TIKI"
     gene            3460..5226
                     /locus_tag="VFU15_06665"
     CDS             3460..5226
                     /locus_tag="VFU15_06665"
                     /inference="COORDINATES: protein motif:HMM:NF024299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU4717494.1"
                     /translation="MNRFFVFVLSAFLVLAGSSCRHQKAATNSGDTNNFGNSPKQRDD
                     AITANKADEQSVFIQGCIEKSLGNYSKALGEFQEVLNMNPDNAAANYEVAGMYWQLNQ
                     ADRALKYAQKATQLNPDNQWYKFRYAELLQVNGKDDEATKIFQDMSDADPGNVDLLYR
                     YAASLTKAKKYDDALKTYDRIEAIQGISDTLTRCRITIYHEQKDIASEEKALVKLVDA
                     FPEEMDYYIRLSDFYTLNGMPAKAAEIFRKMTVVFPYDVTPHLKLADYYYSQKQYDKA
                     FAEAEHGFEEPDDLNEKIDLFNKWYPLADSAPALTPARQKEADSLCRVLRRIHPDEAG
                     VYVLYGNLLYKNGKFKDAREQYHKATSINPDMYEPWKRIMMINDQLKDNATQEKDCKD
                     ILELFPAQPDAYFYLGMIQYGNKQYSQAADNISAGVDYLFDDPQKEMKMDLVLIDAYR
                     HCDKDDKADDLTEKILAKDTANYALMASYSASLLDRGMKLYNAEKMMLKVVAHDPSAA
                     NTETLAWLEFKLSDYAAAKTWMSVALTKDPNNARMNERMGDIQYRLGNADDAMKYWKK
                     AKEKGGSNPDLDKKISTGKLQE"
     gene            5223..6161
                     /locus_tag="VFU15_06670"
     CDS             5223..6161
                     /locus_tag="VFU15_06670"
                     /inference="COORDINATES: protein motif:HMM:NF025490.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4292 domain-containing protein"
                     /protein_id="HEU4717495.1"
                     /translation="MIDNSMPRISILLRAFILSLILVAGSSALSSCRVKKKKKQVAAD
                     TTSRPPGNCRLDRKLPRALISEMRKKEFRFEWLSAKLDCEAGDDSTRFSFDVSLRMRR
                     DSVIWMQIMDPVIGIKVARILITKDSVKFVRYIGDGGLPDCSYFSGDFIYLSQLLGTD
                     VDFELMQSLLIGNSVSFYEDDEKLHSSVNENECNYTLSTIRKRKLKKVLDQQKQPDDP
                     LQTISLDPSTFKIMKILFIDSQNRTFTAEYSDMQKEDSLSFPHHAFFNAKGTQKSASI
                     DITYKKISLNKHFDFPFSIPDDCTPIIIPQDQQDKH"
     gene            6181..7494
                     /locus_tag="VFU15_06675"
     CDS             6181..7494
                     /locus_tag="VFU15_06675"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEU4717496.1"
                     /translation="MFLALLLVATPLGFAQAKKKKKSSKPKVVKQDKSDLELKKKNLQ
                     TQIDLTDKLLKETRRSKTLSLGQLLALNKKIEAREALMAEINSEIASLDSQIAENNSY
                     VTELDSEIAQLKRDYAKMIVFAYRNEDAYQRMMFLFSADDFNQAFLRMRYIQQIGDGR
                     KEQADKIRDRQQELNDEIRALEQQRQDKQQLLGNQQDEKDSLAQEKEDKDKTFRTLQT
                     KETQLNDDLQKKKTEKANIDSAIQKLIAEEAQRAAAAAAAAAKNNPPKNNTGDKGASN
                     PKSNTPASADKTPPLTPDAVKLGNSFAGSQGQLPWPVAQGTITSHFGKQPHAVLKDVY
                     VYNNGIDISTTEGANARAVFDGEVTGVTNIPGSGWLVIVRHGEYLTVYAKLEEVFVKL
                     GDKVKTKENIGKVSTDTDSGETILHFEVWKGVGKLDPEAWLAKGN"
     gene            7611..8099
                     /locus_tag="VFU15_06680"
     CDS             7611..8099
                     /locus_tag="VFU15_06680"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEU4717497.1"
                     /translation="MKTLWLIRHAKSSWNHGSITDIERPLSDRGYKDAGEMAERIKKH
                     IVAPVLVSSPAIRAFSTAIIFSRHLGYDLSRIRISNLLYASGVDQYFSVIRRLEDEFQ
                     SALLFGHNPVISDVLNVTAGLQMDEMPTAAVAGIEYDIDSWKKLPLQTGKLTLFDFPK
                     KK"
     gene            8177..>9214
                     /locus_tag="VFU15_06685"
     CDS             8177..>9214
                     /locus_tag="VFU15_06685"
                     /inference="COORDINATES: protein motif:HMM:NF014553.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HEU4717498.1"
                     /translation="MIKKKKKIPLINREISWLSFNDRVLQEAEDESNPLIERLRFLGI
                     FSNNRDEFFRVRVATLKRMTKLGKKAQELFGGDPKKILDQVQRRVIESSSRFDRVYNS
                     ILHDLEKYHIHIIDEHQLTREQGEWVKEFFRDRIMPNLFPIMVDSAPSFPYLRDKSIY
                     LAVKLERNQRNKKARYALIEIPADTLSRFVVLPARNGHQYIVLLEDAIRFCLREIFPS
                     PEYIRLNAYTIKLTRDAELDIDNDISKSFVEKISGSLKKRKKGAPVRFVFDKAMPTDL
                     LLFLRKKLKVIKHDNIIPGSRYHNFKDFIFFPDLGHKELIWPELHPVSHPRFSPGESM
                     FSTIREKDVLLN"
BASE COUNT         2313 a         2575 c         2404 g         1922 t
ORIGIN      
        1 tcgcctggta ggggcacccc atgaaaacaa ggctcaacgc accgaaaagc actgcaaatt
       61 ttgcggattt catacttcgt tggtttaagg ttaatggttt acaaatttag aagaattttc
      121 ctccctccac cgggcagaag aagggatatg ctgatttttt acccggtcgc gaaatgcatt
      181 caaaaacagc gccaaactgt taaacattcc gaatgcataa ttcccgcgga tactaaaagt
      241 cactctccaa agttattttt gacctcccgt tttcaaaata ctgcattttg cgtatttcct
      301 cagttaacgt tttgccgtgg ataaaaacac gcgcgccttc tcttacacta cttgttttag
      361 agggaacttc gtcgtacttc aaccgtgaat ccgcttaaga aacttgccgg gcagaccgcc
      421 gtgtacggga tgagcaccat cttcggacgg ctgctgacgt atgccctgac gccgttttac
      481 acctacattt tcgggcaccc ggccgacttc ggcatcaaca cggagtttta cgcgtacatg
      541 tcctttctca atgtcttttt cacctacgga atggaaaccg ggctgttcaa tttttccgtt
      601 tcggaacaga acaagggtct tgtttacagc acggcgctgc gctcgctttt cgtttccacg
      661 gttcttttca cgctgccggt gatcctcttc gcaggcccga tcgcggatgt gctgcgttac
      721 ccgggacacg gagaattcgt catctgggtc atccttatcg tagccaccga tgcgcttacg
      781 gcgattccgt tcgcgaagct gcgcgagcag aacaaggcga agcgtttcgc cacggtcaag
      841 ctcgtgaaca tcgccgtcaa cctgctgatc aacttcttcc tcatcggcta ttgcaaatgg
      901 gcatacgagc accatcccga atcgttctgg ggaagcttct acaatcccgc catcggcata
      961 ggctatgcat tcgtggcgca gctcatcgcc aactgtgttt ccgtgctgct gctgctgccc
     1021 gagctggtgc aggtgcgttc ggggttcaac tggccgctgt ggaagcgcat gatgggctat
     1081 tccatgcccc tgctcgttgt cggtcttgcg ggaatgacga atgaaacgat ggaccggata
     1141 cttctgaaat acctgctgcc ggccgatatt tcggaagcgc aggtgggcat ttacggcgcc
     1201 tgctacaaga tctccatcct gatgaccatt ttcatccagg cattcaagta tgcggccgaa
     1261 ccgttttttt tcgcgcagca gaaagagcag gatgcgcata tcctttacgg gcgcgtgctg
     1321 aaatacttcc tgctgttctg cctgctcatt ttcctcggca ccacgctcaa tctttcctgg
     1381 atacagtatt tcgtcggcaa acgataccgc gtgggcatac cggttgtgcc tatcctgctt
     1441 tttgcgaacc tctgcctggg catgtatttc aatctttcca tctggtacaa gctcaccggg
     1501 cggacgcgtt tcgggatgtg gctgacgctt ttcggcgcgc tcataacgct cgtgctgaat
     1561 ttcgcctgga tcccttcgca gggttatttt cacagctaca tgggatcggc ctgggcgacg
     1621 cttatctgtt atgccgtgat gatggtgctg tcgtatattt tcggacagcg ctactaccac
     1681 gtgaaatacg atcttccgcg cctgctcatg tataccgggc tcgcgctcgc actctttttc
     1741 gccggttatt atttcatttc caccggtaaa gcggccaccg acctgctgct gcgcaacctg
     1801 cttttcatcg ttttcatact cgtcattttc attgtggaaa aacccgggag gttgctgcgg
     1861 cgtacttccc ggccgacggg ctaaaatccg taccttcgct ctgatgctga aaatcaaggt
     1921 tatcaaccag tccgggcatc cgctccccga atacgcaacg gctcacgcgg ccggtctcga
     1981 cctgcgcgcc aacctcgaag agcccgtttc cctcagaccg ctcgaacgcg ccgttattcc
     2041 taccggcctt ttcatggaaa taccggaagg atacgaagcg caggtgcgcc cgagaagcgg
     2101 gcttgccgcg aaacacggga tctccgtgct caacagtccc ggaacgatcg acgccgatta
     2161 ccggggagag atcaaggtca ttcttgtcaa tctttccaat agcgcgttca ccgtcgagaa
     2221 tggggagcgc attgcgcaga tcgtatttgc aaagcacgag caggtggaat ggatctcctc
     2281 cgtggagctg acaacatcag aacgcggcag cggcggattc ggccacaccg gcaccaaatg
     2341 aaaccatacc cgttaccggt aagcagctgc agctgcgaaa gatggtaaca gaacagaata
     2401 atcactatca aatcatcagt ttgttcccgg ttttatgaag atcattatac caatggcggg
     2461 catgggaaaa aggatgcgcc cgcatacgct gaccgtacct aaaccgctga tccccgttgc
     2521 cgggaaaccg atcgtggagc gcctcgtgga agacatcgca caggtgtgca gcgaaccgct
     2581 ggaagagatc gccttcatca tcgggccctc gttcggaaaa gaagtggaag ccatgctcgt
     2641 gaatgtggcc ggccgcctcg gcgcgaaagg cagcatctgg taccaggaag aagcgctcgg
     2701 aaccgcgcac gccatcatgt gcgcagcgcc cgtgctcgac ggaaaagtgg tcgtcgcatt
     2761 tgccgacacg cttttcaaag ccgacttcag gctcgattct tcgaaagaag ggatcatctg
     2821 ggtacagaag atcgacgatc cgcgcgcatt cggcgtggtg aaggtgaatg cggaaaacgt
     2881 catcacggat ttcgtggaga agccgcagac gttcgtcagc gatctcgcca tcatcggcat
     2941 ttattatttc cgcgacgggg cgaacctgcg cagcgagctg caatacctgc tcgacaacaa
     3001 catccgcgac aagggagaat tccagcttac gaacgcgctg gaaaacatga aatcgaaagg
     3061 tgtgaaattc tcgccgggga aagtcaccga atggctcgac tgcggcaaca aagacgccac
     3121 cgtgtacacc aaccagcgca tgctggaatt caagaaggat gaagaggcgc ttgtttcctc
     3181 ctcggtgcgt tccgataatt cggagatcat cccgccctgc tacatcggcg agaatgtctc
     3241 cctcataaat acgacggtcg gtccgcatgc ttccatcggc agcaactgca ccctcgtcaa
     3301 ttcgtttgtt tcgaacagca tcgtccagcc ggacacaaag atcaccgacg ccaccatcag
     3361 gaactcgatg atcggcaaag cggcggaaat aacaggcggg ggcgaaacgc tcgacgtgag
     3421 catcagcgat tattcgacga taaaaatctg atccgaaaaa tgaaccggtt tttcgtattc
     3481 gtgctgtcgg ctttccttgt tcttgcggga tcttcctgca ggcaccagaa ggccgcgacg
     3541 aattccggcg atacgaacaa tttcgggaat tccccgaagc agcgcgacga cgcgatcaca
     3601 gcgaacaagg ccgatgaaca atccgttttc atccagggct gcattgaaaa atcgctgggg
     3661 aattacagca aggcgctcgg cgagttccag gaagtgctga acatgaatcc cgacaatgct
     3721 gccgcgaatt acgaagtggc cggcatgtac tggcagctca accaggccga ccgcgcgctg
     3781 aaatacgccc agaaagccac gcagctcaac cctgataacc agtggtacaa attccgctac
     3841 gcggagctgc tgcaggtgaa cgggaaagac gacgaggcca cgaagatttt ccaggacatg
     3901 tcggacgctg atccgggaaa cgtggatttg ctgtaccgct atgccgcatc gctcacgaaa
     3961 gcgaaaaaat acgacgacgc gctgaagacc tacgacagga tcgaagcgat ccagggaatt
     4021 tcggatacgc tcacccgctg caggatcacc atttaccacg agcagaaaga catcgcctcg
     4081 gaagaaaaag cgctggtgaa gctcgtcgat gcgttcccgg aagaaatgga ttattacatc
     4141 cgcctttccg atttctatac gctgaacggc atgcccgcga aagccgctga gattttccgg
     4201 aagatgacgg tggtgtttcc gtacgacgtg acgccgcacc tgaagctcgc cgattattat
     4261 tattcgcaga agcaatacga caaagcgttt gcagaagcag agcacgggtt cgaggagccg
     4321 gatgatctca atgaaaagat cgacctcttc aacaagtggt acccgcttgc ggattccgct
     4381 cctgcgctca cgccggcccg ccagaaggaa gcggattcgc tctgccgcgt attgagaagg
     4441 atccacccgg atgaagccgg cgtatacgtg ctgtacggga acctgcttta taagaacggg
     4501 aaattcaaag atgcgcgcga acagtatcac aaggcgacgt cgatcaatcc cgacatgtac
     4561 gagccgtgga agcggatcat gatgatcaac gaccagctga aggataatgc aacgcaggaa
     4621 aaagactgca aggacattct cgaacttttc ccggcgcagc ccgatgctta tttctacctc
     4681 ggcatgatcc agtacggcaa taagcagtac agccaggcgg ccgataatat ttccgccggc
     4741 gtggattatc ttttcgatga tccgcagaaa gaaatgaaaa tggatctcgt gctcatcgat
     4801 gcgtaccggc attgcgacaa ggatgacaaa gcggatgatc tcacggaaaa aattctcgcg
     4861 aaagacacgg cgaactatgc gctcatggct tcctattccg cgtcactgct cgaccgcggg
     4921 atgaagctgt acaatgcgga aaaaatgatg ctcaaagtcg tggcgcatga tccttccgcc
     4981 gcgaatacgg aaacgctggc ctggctcgaa ttcaagcttt cggattacgc cgccgccaaa
     5041 acgtggatga gcgttgcgct gacaaaagac ccgaacaacg cacgcatgaa cgagcgcatg
     5101 ggcgacatcc agtacaggct cggcaacgcg gacgacgcga tgaaatactg gaaaaaagca
     5161 aaagaaaaag gcggtagcaa tcccgatctc gataaaaaaa taagcaccgg aaaattgcag
     5221 gaatgattga caacagcatg ccccgtattt ccattctcct gagagcgttc attttatcgc
     5281 tcatcctcgt cgccggctca tcggccctgt catcgtgccg ggtgaagaaa aagaaaaagc
     5341 aggtagccgc cgatacaacg agccgcccgc cgggaaactg ccgcctcgac cggaagctgc
     5401 cgcgcgcgct gatcagcgag atgcggaaaa aagaattccg tttcgaatgg ctttccgcca
     5461 agctcgattg cgaagccggt gacgacagca cgaggttcag tttcgacgtc agcctgcgca
     5521 tgcgccgcga cagcgtgatc tggatgcaga tcatggaccc ggtgatcggc atcaaggtgg
     5581 cgcggatcct cattacgaaa gacagcgtga aattcgtgcg ctacatcggc gacggcggcc
     5641 ttcccgattg ctcgtatttc tccggcgact ttatttacct gagccagctc ctcggcacgg
     5701 acgtggattt cgaactgatg cagtcgctgc tgatcggcaa cagcgtttcg ttctacgaag
     5761 acgacgaaaa actgcattct tccgtgaacg agaacgaatg caactatacg ctgagcacca
     5821 tccgcaaacg caagctgaaa aaagtgctcg accagcagaa gcagccggac gatccgctgc
     5881 agacaatatc gctcgaccct tccacgttta agatcatgaa gatcctgttc atcgattccc
     5941 agaaccggac ctttaccgcg gaatacagcg acatgcagaa agaggattcg ctttcgttcc
     6001 cgcaccatgc gttcttcaat gcaaaaggaa cgcagaagtc ggccagcatt gatatcactt
     6061 acaagaaaat ctcgctgaac aaacatttcg attttccatt ctcgataccc gatgactgca
     6121 ccccgatcat tattccccag gatcagcagg ataagcatta agcacctggc ctcggtcgcg
     6181 atgtttcttg ccctgctgct tgtagctacg cctttgggct ttgcgcaggc gaagaaaaag
     6241 aagaaatcgt cgaagccgaa agtggtgaag caggacaaat ccgatctcga gctgaagaaa
     6301 aaaaatctcc agacgcagat cgatctcacc gataaactgc tgaaagaaac gaggcgcagc
     6361 aaaacccttt cgctcgggca gctgcttgcg ctgaacaaga agatcgaagc gcgtgaagcg
     6421 ctgatggcgg agatcaacag cgagatcgct tcgctcgaca gccagatagc ggaaaataat
     6481 tcgtacgtca cggagctcga ttccgagatc gcccagctga agcgcgatta cgcgaaaatg
     6541 atcgtgttcg cctaccggaa cgaagacgcc taccagcgga tgatgtttct cttttccgct
     6601 gatgatttca accaggcctt tctccgcatg cgttatatcc agcagatcgg cgacggaaga
     6661 aaagagcagg cagacaagat ccgtgaccgg cagcaggagc tgaacgacga gatccgcgcg
     6721 ctggagcagc agcgccagga caagcaacag ctgcttggca accagcagga cgaaaaagat
     6781 tcgctggcgc aggagaaaga agacaaggat aaaacattcc gcaccctgca gacgaaagaa
     6841 acgcagctca atgacgatct gcagaaaaag aaaaccgaaa aagccaatat cgattccgcc
     6901 atccagaagc tcatcgccga ggaagcccag cgcgcagcag cggccgctgc cgccgccgcg
     6961 aaaaataatc cgccgaaaaa caacaccggc gacaaaggcg catcgaaccc gaaaagcaac
     7021 acgcccgctt ccgccgacaa aacgcccccg cttacacccg atgccgtgaa gctgggcaac
     7081 agcttcgcgg gcagccaggg acagctcccc tggcccgttg cgcagggaac gatcaccagc
     7141 catttcggga aacagccgca tgccgtgctg aaagacgttt acgtgtacaa caacgggatc
     7201 gacatcagca cgacggaagg ggcgaatgcg cgcgccgtat tcgacgggga agtaacaggc
     7261 gtgacgaaca ttcccggttc cgggtggctt gtcatcgtgc gccacggcga atatcttacc
     7321 gtatatgcga aactcgaaga ggtgttcgtg aagctgggcg acaaagtgaa gacgaaagag
     7381 aacatcggga aagtctctac cgatacggat tcgggcgaaa ccattcttca tttcgaagtg
     7441 tggaaaggcg tcggcaagct cgatcccgaa gcgtggctgg ccaaagggaa ctgagcggcg
     7501 ttttttcacg gaagaatttt cacagggctt cccggcaccg ggaacgctat tttcccggga
     7561 cgctgcattt cccgttggca aaaccggcat ctcttgttat cttgtgagca atgaaaacgc
     7621 tctggctcat caggcatgcg aagtcgtcgt ggaaccacgg gagcatcacc gacatcgagc
     7681 ggccgctcag cgaccggggc tataaggatg cgggcgaaat ggcggaacgc atcaagaagc
     7741 acatcgttgc gcccgtgctg gtttcgagtc ccgccatccg cgcgttttcc accgccatca
     7801 ttttttcccg ccatctcggg tatgatcttt cacggatccg catcagcaat ctcctgtatg
     7861 cgtcgggcgt ggaccagtat ttttccgtga tccggcggct ggaagatgaa ttccagtcgg
     7921 cgctgctttt cggtcacaac ccggtgatct cggacgtact gaacgtcact gcaggattgc
     7981 agatggatga aatgccgaca gccgcggtag ccggtatcga gtacgatatc gactcgtgga
     8041 aaaagctgcc gctgcaaacc gggaagctga cgctgttcga ttttcccaaa aaaaaatagc
     8101 cctgcaggtt aaataattct taagtccgtc gctgattcac attccacgtt aattttacgc
     8161 ccgcacgaag gctgccatga tcaagaagaa gaagaaaatt ccgttaatca atcgcgagat
     8221 cagctggctt tcctttaacg accgcgtgct gcaggaagca gaagatgaat ccaacccgct
     8281 gatcgaacgg cttcgtttcc tcggcatttt ttccaacaac cgtgacgaat ttttccgtgt
     8341 acgcgtggcc acgctgaagc gcatgaccaa gctcgggaaa aaagcgcagg agcttttcgg
     8401 cggcgatccg aaaaaaattc tcgaccaggt acagcggcgc gtgatcgaat catcgtcacg
     8461 tttcgaccgc gtgtacaatt ccatcctgca cgaccttgaa aaatatcaca tccacatcat
     8521 cgacgagcac cagctgacgc gcgagcaggg tgaatgggtg aaggaatttt tccgcgaccg
     8581 gatcatgccg aacctcttcc ccatcatggt cgacagcgcg ccctcgttcc cctacctgcg
     8641 cgacaaatcc atttacctcg ccgtaaagct cgagcgcaac cagcggaaca aaaaagcgcg
     8701 ctatgcgctg atcgagatac cggccgatac actttcccgc ttcgtcgttc ttcccgcgcg
     8761 caacgggcac cagtacatcg tgctgctcga agatgcgatc cgtttctgcc tgcgcgagat
     8821 cttcccttcg ccggaataca tccgcctgaa cgcttacacg atcaagctca cgcgcgatgc
     8881 cgagctcgac atcgacaacg acatctcgaa gagcttcgtg gaaaaaattt ccggcagtct
     8941 caaaaagcgg aagaaaggcg cgccggtccg tttcgtattc gacaaagcga tgccgacgga
     9001 ccttctcctg ttcctgcgca aaaagctgaa agtgatcaag cacgacaaca tcatcccggg
     9061 atcgcgctat cacaatttca aggatttcat cttctttccc gacctcggcc acaaggagct
     9121 catctggccc gagctgcatc ctgtttcgca tccgcgtttt tcaccgggcg aaagcatgtt
     9181 cagcacgata cgggaaaaag atgtcctgct caat
//
LOCUS       DASTSF010000228         2626 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_266462,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000228 DASTSF010000000
VERSION     DASTSF010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2626
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_266462"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..680)
                     /locus_tag="VFU15_06690"
     CDS             complement(<1..680)
                     /locus_tag="VFU15_06690"
                     /inference="COORDINATES: protein motif:HMM:NF019268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEU4717499.1"
                     /translation="MKTKLLGIALLAGSMTAFAQDDSTAYEKKVEKSAPYAWGDFTWV
                     QGNSRLHRQVLDSKYFTGDVTMDMNYNYNFAHPIDHTESGSTACFRTDEFNVSYIEAG
                     GDFHDPASGARARLMLQFGARAVGVPRNDATPLRGQYDLYTALRFVTEGYVGQHFKVW
                     HGVNVDVGIFKSYVGLMGYNNFENWNYQNSFTSDNTPWFFTGARIQTFPTDKLKIEYW
                     IVNGWQTYA"
     gene            complement(776..2473)
                     /gene="kdpA"
                     /locus_tag="VFU15_06695"
     CDS             complement(776..2473)
                     /gene="kdpA"
                     /locus_tag="VFU15_06695"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HEU4717500.1"
                     /translation="MISITDIAMMSVIFAGAVALAIPYGKYIAKVYAGERTFLDRVFN
                     PIEKIIFRFLGVDAMKEMTWKQNLKALLTVNIIWFPLTMIILMTQKWLPLNPDHIPNM
                     PIDLAFHSTCAFITNCFQQHYSGETGMSYLSQLVIMFSLFFLTPAVTLAAMAVVFNAL
                     KERTTEVLGNFYNYYLKSITRILLPVALVTAIVFLFHGLPMTFHGAHSFTTLQGDSAH
                     VARGPLAAFESIKMLGLDGGGFFGANSAHPFENPDFFTNIIENIFHLLLPIATVFALG
                     FYLNRKRLAWMIFGVMTLGFFLLLIPALHYEMPGNPAVAHMGIDNSLGNMEGKEVRFG
                     TAASTLWSITATSSMNGGVNSMHDSYMPMAGGMQLLGIMVNSLYGGVGTGVLGFLVMI
                     ILAVFIGSLMIGRTGQFLGKRIGMREIKIAVIVTLLHPLLSLGGAALACSVPSVTTPT
                     LNNPGYHGFSEMIYEYTSASSADGSGFEGLGDSTPWWNISCGIVILLGRFLPIIGAVA
                     IAGILASKKFLPDSSGTLRTDTPTFGLMVFSLIFIVSALAFFAALSIGPFAEHFSMK"
BASE COUNT          682 a          628 c          612 g          704 t
ORIGIN      
        1 gcatacgtct gccagccgtt cacgatccag tattctattt tcagtttatc ggtcgggaat
       61 gtctggatac gcgcgccggt gaagaaccag ggcgtgttat ccgatgtgaa cgaattctgg
      121 taattccagt tctcgaaatt attatagccc atcagcccga cgtacgattt gaaaatgccc
      181 acatccacgt taacgccatg ccagaccttg aaatgctgcc cgacatatcc ttccgttaca
      241 aagcggagcg ccgtgtacag gtcgtattgc ccgcggagcg gcgttgcgtc attgcgcgga
      301 acgcctacag cgcgtgcgcc gaactggagc atgagtcgtg cgcgtgcgcc gctcgccgga
      361 tcgtgaaaat caccgcccgc ttctatgtac gaaacgttga attcgtcggt gcggaaacag
      421 gctgtcgagc cgctttccgt atgatcgatg ggatgagcga aattgtaatt gtagttcatg
      481 tccatcgtaa cgtcgccggt gaaatatttg gaatcgagca cctggcggtg caggcggctg
      541 tttccctgta cccaggtaaa atcgccccac gcataaggag cgcttttctc aacttttttt
      601 tcataagcgg ttgagtcatc ttgtgcaaat gctgtcattg atcctgcaag gagagctatg
      661 ccgagtaatt tggttttcat tttgtttttg tttgctgatg tttcgttacg ttttattttt
      721 attttattgc tggtttttat ttgaattata tttcgtcatt ggttttttct cctggttatt
      781 tcatactgaa atgttccgcg aatggcccaa ttgaaagtgc ggcgaagaac gcgagtgccg
      841 aaacgatgaa gatgagtgag aacaccatca acccgaaagt cggcgtatct gtcctgagcg
      901 ttcctgaact atccggcagg aattttttcg atgcaagaat tcccgcaatg gcaacagcac
      961 cgatgatcgg taaaaatctt ccaagcagga tcacaattcc gcagctgata ttccaccacg
     1021 gcgtgctatc acccaatcct tcaaacccgc ttccgtctgc cgatgaagct gaagtgtatt
     1081 cgtaaatcat ttcactgaat ccatgatagc ccgggttgtt cagtgttggt gttgtgactg
     1141 acggaacact gcacgcgagt gctgctcccc cgagcgataa cagcggatgc aaaagcgtta
     1201 cgatcaccgc aatttttatt tcccgcatcc cgattctttt cccgagaaat tgcccggttc
     1261 ttccgatcat caggcttccg atgaaaacag caaggatgat catcaccagg aatccaagga
     1321 ctcctgttcc tactccgccg taaagtgaat tgaccatgat gccgagcaat tgcattccac
     1381 ccgccatcgg catatagcta tcgtgcatac tattcacgcc accgttcatg gaagatgtag
     1441 ctgtaatgct ccacaatgta gaagcggctg taccaaagcg tacttccttt ccttccatat
     1501 ttcccaatga attatcaatg cccatgtgcg ccactgcagg atttcccggc atttcataat
     1561 gaagtgccgg aatgagcagc aggaaaaacc ctaatgtcat tacgccgaaa atcatccacg
     1621 ccagtctttt cctgttgaga taaaagccga gagcgaaaac ggttgcaatc gggagcagca
     1681 ggtgaaaaat attttctatg atgttggtga agaaatcggg attctcgaac ggatgcgcgg
     1741 aatttgcccc gaaaaatccg cctccgtcaa gtccaagcat cttgatggat tcaaatgcgg
     1801 cgagcggccc tcttgcaaca tgcgcactgt caccctgcag tgttgtgaag gaatgcgctc
     1861 catgaaaggt catgggcaat ccgtgaaaga gaaaaacaat tgctgtaaca agagcaacgg
     1921 gaagcagaat tcttgtaatt gatttcagat aataattgta gaaattcccg agcacttctg
     1981 ttgttctttc tttcagcgcg ttgaacacaa ctgccatagc agcaagtgta actgccggtg
     2041 tcaggaagaa cagcgagaac ataataacaa gctgcgacag gtaactcatt cctgtttcgc
     2101 cggaataatg ctgctgaaaa caattcgtga tgaaagcgca ggtagaatga aacgcaagat
     2161 cgatcggcat gttcggaata tgatccgggt tcagtggcag ccatttctgc gtcatgagaa
     2221 taatcatcgt gagcgggaac cagatgatgt tcaccgtgag caatgctttc aggttctgct
     2281 tccatgtcat ttccttcatc gcatccacac cgaggaaacg gaaaatgatt ttctcaatcg
     2341 gattgaaaac ccggtcgagg aaagttcttt ctcccgcgta aactttagca atatattttc
     2401 catacggaat agcaagcgct accgccccgg caaaaatcac cgacatcatt gctatatccg
     2461 ttatggatat cattttttac tgttttaaaa tttggaggtg aggcctattg taagcaatcc
     2521 gtctttattt gttttgttga ggtgctccgt ataaaataca ttgtaatccg accagaaata
     2581 acgcgcttcc aatcgcagtt gcgtgagact ggtcaacgaa atatta
//
LOCUS       DASTSF010000229         4411 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_926120,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000229 DASTSF010000000
VERSION     DASTSF010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4411
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_926120"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            190..3441
                     /locus_tag="VFU15_06700"
     CDS             190..3441
                     /locus_tag="VFU15_06700"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEU4717501.1"
                     /translation="MKKSKLLFAGSGIAAVGLAVIMGLSLNHDAVSKYKPSSHFAISS
                     SEDDDAEGAAGAAQWRFDRIKNQATNDLDYQEMINVQNMANTAISNARQGNSQVSASS
                     AQWNEIGPDNVGGRTRAILIDKDNHNHMFAGGVSGGLFESTDGGNNWTPCPGFWNAAN
                     VNLNVTTIDQAANGDIYVGTGEAPDLYFAFGTGAGGFLGGGVFKSTDHGATFNLLTST
                     TPTYNQTGSQWAGVNRIACDPTDATGQIVLVGTSRGLKLSTDGGASWSSPTGPVSAAS
                     TKDVKFGSDGSCVAVVSGTPWRSTDHGNSFTNVGTSAQGFYSNAAIGRTEIAFAPSDP
                     NYVYAIVASSTGALGGVYCSTDNAQTWTAIAGASTSAFDPFSYTEKQGNYDNTIAVDP
                     FDKKHIVVGGVELWRWDMVSNSPPSGQWTRIALEFAGLTNPFYVHSDKHAIVFHPTNQ
                     GEFFVGTDGGIFRTTDNGLYYNSMNFNYNVTQCYDVAIDHWAPNRDMSMAGCQDNGTQ
                     YVDGTGNDAKWAKDVNGGDGGGVEISYLNPNACFSTIYYGQLFRSNNRGNSSSAFYDS
                     QITGAGTNLAGFVTPIRLWESAVDYLSPDSVIIANRQVVQNKHVTDGSTASYSGTLSL
                     SYPTANPAPTIILDSVMFTCGNDTVNSDGAGVLSGDGFGSVQTNGNYTVTFNTTPAAN
                     KTVKAFFYMQYGAGTDFTVFSNVQGRLLDYVTPVQVNADDTVKIQDIIQSRLSVGCAS
                     AIWMTRRPIDFSTTPMWIKVAGSHSTPSAFSGTVTCQAWSPDGNHLYVGTDGGGVYRI
                     DNVGLCKDTIDGNVEFTGNIANPNCITRCYRIGAWTSRTVTGIDVDPNDPSNVVVSVG
                     NYGNTAYVYYADHGDTATVVGTTFVNKTGTLYSGGGAPAYCVSFDKFNPGRVLVGTEH
                     GVYETQNITASSPSFAYASNGPGPVVVDAIRQERWEPWQVSNSGCFYIGTHGRGMWRD
                     DSSWQQPTGIYSPSQPGNDNSSSSANHDLKVFPNPVVDNSNVTFRLDNAGAATVSIYD
                     LSGKLVSSKEYQDLTAGNNTVQFGTDGFTKGTYIIMVTQGAKKIGTGRFLKMD"
     gene            complement(3522..4304)
                     /locus_tag="VFU15_06705"
     CDS             complement(3522..4304)
                     /locus_tag="VFU15_06705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717502.1"
                     /translation="MPTRLPVFAFLLLVVFAFTGDGSIFIREPYDFFTTDNLGNVYLV
                     KNSVIRKFDSKGVFQKEFSNKNFGPVTSFDATNPLRLVVFYRDFSRVVFLDNTLSQNG
                     EPVALESLGFPLATLVCSSHDNGLWIYDQQNSELIRFDRNLQVENRTGNITQLTGQTL
                     RPSFLLEADNKLFLDNPSSGALVFDALGTYSQTLPVDSVRSFQVSDGSLYFFAHRKFT
                     GMPISPGDLTVLPVSDTLVTGYRMEKNAFYAGTSEGVKIIPR"
BASE COUNT         1088 a         1117 c         1170 g         1036 t
ORIGIN      
        1 ctattgactg ggccttttcg gttgcgtata tttgtatata atgtgttgtt tttatcatcc
       61 ccccttaatg cagcaggagg ctgctttaaa taaatttctc agtttggtgt tctgttaaaa
      121 aaccttagtt ttggatgctt attttcataa gcaaagaata catcagaggg aattttaaac
      181 ttatcacaaa tgaaaaaaag caaactgctg tttgctggct ccggcattgc tgcggtcggg
      241 ctggctgtaa taatgggtct ttccctgaat cacgacgctg tttcgaagta taaaccttcg
      301 tcacacttcg cgataagctc ttcggaagac gatgatgcgg aaggtgcagc aggtgcagcc
      361 caatggcgtt ttgacaggat caagaaccag gcgaccaacg accttgatta ccaggaaatg
      421 atcaatgtcc agaacatggc gaacaccgcc atctccaatg cgcgccaggg taattcacag
      481 gtttctgcca gttccgccca gtggaatgag atcggtcccg ataacgtggg tggaagaacc
      541 cgcgcaatac tgatcgataa ggacaatcac aatcacatgt ttgccggcgg cgtgagcggc
      601 ggacttttcg agtctacgga cggtggtaac aactggacgc cgtgtcccgg tttctggaac
      661 gcagccaacg tgaacctgaa tgttactacg atcgaccagg cagccaatgg tgatatttac
      721 gtaggaaccg gtgaagcgcc ggatctgtat tttgcgtttg gaaccggtgc cggaggtttt
      781 ctcggtggcg gcgttttcaa atccaccgac cacggtgcga cgttcaacct gctgacatca
      841 acaacgccta catataacca gaccggctca caatgggcgg gagtcaaccg gatcgcctgc
      901 gatccgacag atgcaaccgg gcagattgta cttgtgggca ccagccgcgg acttaagctc
      961 agcacagacg gcggagcatc atggagctcc ccgacgggtc ccgtatctgc tgcatccacc
     1021 aaagacgtaa aattcggctc tgacggatcc tgcgttgcgg tggtaagcgg aacgccatgg
     1081 cgctctaccg atcacggtaa cagctttacg aatgtgggaa caagcgcgca gggcttttat
     1141 tccaatgcag cgatcggaag aacggaaatt gcgtttgccc ccagcgatcc gaattatgtt
     1201 tatgcgatcg ttgcgtcctc aacaggcgcc cttggcggtg tctattgttc cacggacaat
     1261 gcgcagacct ggactgccat cgcaggtgcg agcacttcgg cattcgatcc tttcagctat
     1321 actgaaaagc agggaaacta cgacaatacg atcgcggttg atcctttcga taaaaaacat
     1381 atcgttgtag gtggggttga gctgtggcga tgggatatgg tgagcaattc acctccctca
     1441 ggtcagtgga caaggatcgc gctcgagttc gcaggattga caaatccttt ttatgtgcac
     1501 tcggataagc atgcgatcgt attccatccg acgaaccagg gtgaattttt tgtgggtacg
     1561 gacggcggaa ttttccgcac gaccgacaac ggtctttact acaactccat gaacttcaat
     1621 tataatgtca cacagtgcta tgacgttgcg attgatcatt gggcccccaa cagggatatg
     1681 tcaatggcag gttgccagga taacggaacg cagtacgttg acggaaccgg taacgatgcc
     1741 aagtgggcaa aagacgttaa tggcggtgac ggcggcggtg ttgaaatttc ctacctgaac
     1801 ccgaacgcct gcttctcgac catttattat ggccagcttt tcaggtcgaa taaccgcggg
     1861 aacagttcct ctgcgttcta cgattcgcag atcaccggag cgggaacaaa ccttgccggc
     1921 tttgttacgc cgatccgtct ttgggaaagc gctgtcgatt acctcagccc tgattcggtc
     1981 attattgcta atagacaggt tgtgcagaac aagcatgtta ccgatggttc gacggcatct
     2041 tattccggaa cgctttcgct ttcctatccc actgcaaacc cggcaccgac tatcatcctc
     2101 gatagcgtga tgttcacctg cggaaacgac acagtgaatt cggatggcgc cggggttctt
     2161 tccggcgatg gattcggttc tgttcagacc aacgggaact acacggttac gttcaacaca
     2221 acgcctgctg cgaacaagac agtgaaagca ttcttctata tgcagtacgg agcaggaacc
     2281 gattttacgg ttttctccaa tgtacagggc cgcctgcttg attatgtaac accagtgcag
     2341 gttaatgcgg atgacacggt gaagatccag gatattatcc agtcgcgcct gtctgtcggt
     2401 tgtgccagtg caatctggat gacgcgccgg ccgatcgatt tctcaaccac acctatgtgg
     2461 attaaagtcg cagggtcgca ttccacgcct tccgctttct ccggtacggt aacctgccag
     2521 gcatggtcgc ctgacggaaa ccacctgtac gtgggaacgg acggtggcgg tgtttaccgt
     2581 attgataatg tcggcctgtg caaagacacg atcgacggaa acgttgagtt taccggcaat
     2641 atcgccaacc cgaactgcat cacgcgctgc tatcgcatcg gtgcatggac ttcacgtacc
     2701 gtaacaggta tcgatgtcga tccgaacgat ccgagcaacg tagtggtttc tgttggtaac
     2761 tacggcaaca cagcttacgt ttattacgcc gatcatgggg ataccgcaac ggtcgttggc
     2821 actacgttcg tcaataaaac cggcacactt tattcaggtg gtggcgcacc tgcttactgc
     2881 gtcagctttg acaagttcaa cccgggacgc gtactcgtgg gtacagagca cggtgtttat
     2941 gaaacacaga acatcaccgc ttccagtccc agcttcgctt atgcaagcaa cggacccggc
     3001 ccggtggttg tcgatgccat ccgccaggaa cgctgggagc cctggcaggt cagcaattcg
     3061 ggatgtttct acattggaac gcacggccgc ggcatgtggc gtgatgattc atcctggcag
     3121 cagcctacgg gaatctattc tccgtcacaa cccggaaacg ataattcgtc ttccagtgcg
     3181 aatcacgacc tgaaagtgtt cccgaaccct gtggtcgaca attcgaacgt aacgttccgt
     3241 ctcgacaatg caggagctgc cacggtgagc atttatgacc tcagcggcaa gctggtctcc
     3301 tcaaaggaat accaggacct taccgcgggt aataataccg ttcagttcgg tactgacgga
     3361 ttcaccaaag gaacctacat catcatggtt acccagggtg cgaagaagat cggcacggga
     3421 agattcctga aaatggattg atcatcttcc ggtcagaaat aaaagacccc ggcagttttg
     3481 ccggggtttc tttttagaaa cgtgtgctga aaaaaggcag cttatctcgg gatgatcttc
     3541 acaccttcgg aagtaccggc gtagaatgcg ttcttctcca tgcggtagcc ggtaaccagt
     3601 gtgtcgctga cgggaagaac cgtcaggtcg cccgggctga taggcattcc tgtaaatttc
     3661 cggtgcgcga aaaagtataa ggatccgtcg ctgacctgga aggaccgcac gctgtccacc
     3721 ggcaacgttt gcgaataagt tcccagcgca tcgaacacaa gcgcacctga agacgggtta
     3781 tccaggaaaa gcttgttgtc agcttcaagc aggaacgaag ggcgaagtgt ttgcccggtg
     3841 agctgcgtaa tgtttcccgt gcggttttcc acctgcaggt tgcggtcgaa acggatcagc
     3901 tcggaattct gctggtcata aatccaaagt ccattgtcat gcgaagagca taccagcgtg
     3961 gcaagcggaa atccaagcga ttcgagcgcg accggttccc cgttctggct taacgtattg
     4021 tcgaggaaaa cgacgcggct gaaatcgcgg taaaaaacaa cgagacgcaa agggttcgtt
     4081 gcatcgaaag aggttacggg tccgaaattt ttgttgctga actctttctg gaaaacacct
     4141 ttgctgtcga atttccggat aacggaattt ttgacgaggt aaacattgcc gagattatcc
     4201 gtcgtgaaaa aatcatacgg ttcccggatg aaaatggaac cgtcacccgt aaaggcgaaa
     4261 accacgagaa gcaggaaagc gaaaaccggt aaccgcgtcg gcatgattca aatgtaacgc
     4321 aaaagtcaga ccacaccttc aggcgttctg ctgcaggatc ccgtcgatgt atttccggct
     4381 gttcgaaagg cgcgggatct tgttctgccc g
//
LOCUS       DASTSF010000230         2683 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_17760,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000230 DASTSF010000000
VERSION     DASTSF010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2683
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17760"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1401
                     /locus_tag="VFU15_06710"
     CDS             <1..1401
                     /locus_tag="VFU15_06710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717503.1"
                     /translation="SRTAAYCSDGLLRSDGRYIIPPRKFSGCRLVGTDHGLALFAVYR
                     NIWKEGLIDGNGTVRLKRKWADISSVHNGYASVCRRDRMLFYEDCYNILDMNNGERLL
                     PRKLRSRGAVLLSDSSAIVRTKKEPRVYSLNKKEFVTGHGMNDVQLLDSTGHAIAVKT
                     CMGHIGIIDAEGKLIADTASDAITYVYSPSGNIRQYVFYADRHHGQIFDAVTGRMSDD
                     TASCRRLVATAMQNVVMDSAGDVLVKNTQCPSLCFGKKAGTARLASWQEDVLFDSLYA
                     PVRFIPDTDYYLLGTFMCAYCMNKSPQYFFYRWAKDYERDNGHYYKMDYVNDSLLSVK
                     RGYTFNDYYDYNWRGLPAYSENDDLFFVTMLFNDGPHAMLLDSLFTGNDWKTIITNEI
                     LACLDSAKGVEGNCSNPFLFSVMCRERFLVTPEHLALFPPSYMKNGTQLEVDIPWSKL
                     SPYLRKDVASKIGVRD"
     gene            1706..>2683
                     /locus_tag="VFU15_06715"
     CDS             1706..>2683
                     /locus_tag="VFU15_06715"
                     /inference="COORDINATES: protein motif:HMM:NF012340.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HEU4717504.1"
                     /translation="MKTVSRIISLAAAVTFAFSLNAQDKTNKKDSQIKFTVVSDAKCT
                     SVKDQYHSGTCWCFSTESFLESEFMRMGKGPLDLSEMWIVRAGYIEKAKKYMRMMGKT
                     NFGPGGEPGDVISLSAQYGIVPQDAYPGLPEGETKIRHGEMDDVLKAILDAQLKLEDG
                     KLSPNWLAAYTAALDAYLGAPPANFTVNGKSYTPKSYADFLGFKPEDYVAITSFTHHP
                     FYTQFPLEIPDNWSWSPYYNVPLSDLTSIADNATMNGYTVAWGADVSEGYCSPKNGMA
                     IVPNKNWDLMRKGEKDSLWILPSKDSIITQDVRQAAFDNLSTQDDHGMQL"
BASE COUNT          674 a          738 c          678 g          593 t
ORIGIN      
        1 tcacgaaccg cagcttactg cagcgatggc ctgctgcgaa gcgacggccg atacatcatt
       61 cctccccgaa agttctccgg gtgcaggctt gtcggaacgg atcacggcct ggcgcttttt
      121 gcggtttacc ggaatatctg gaaagaaggc ctcatcgacg ggaacggaac tgtacgcctg
      181 aaacgaaaat gggcggacat ttcctcggtg cacaacggat acgcatccgt ctgccggcgc
      241 gacaggatgc tgttttacga agattgctac aacatcctgg acatgaacaa cggtgaacgc
      301 ctgctgccgc gcaagcttcg ttcccgggga gccgtactgc tcagcgacag ctcagccatc
      361 gtgagaacca aaaaagagcc gcgcgtttat tcgctgaaca aaaaggaatt cgtgaccgga
      421 cacgggatga acgatgtcca gctactcgac agtacggggc atgcgatagc cgtcaaaaca
      481 tgcatgggac acatcgggat catcgatgcg gaggggaagc tgatcgcgga caccgcgagc
      541 gatgccatca cctatgttta ttcgccgtcg ggaaacatac ggcaatatgt tttttatgcc
      601 gaccgtcatc acggacagat cttcgatgcg gttacggggc ggatgagcga cgacacggcc
      661 tcctgccggc gactcgttgc tacagcaatg cagaacgtgg tgatggattc ggccggtgac
      721 gtcctcgtga aaaacacaca atgcccttct ctctgtttcg gaaaaaaagc cggaacagcg
      781 cggctggcgt catggcagga agatgtcctc ttcgattcgc tgtacgcacc ggtccgtttc
      841 atcccggaca cagattatta tctcttgggc acgttcatgt gcgcatactg catgaacaaa
      901 agtcctcagt attttttcta caggtgggcg aaggattacg agcgcgacaa cgggcattat
      961 tacaaaatgg attacgtaaa tgattccctg ctcagtgtaa agcgcggata cactttcaac
     1021 gattattacg attacaactg gcgcggcctt ccggcctatt ccgagaacga tgacctgttc
     1081 ttcgtaacga tgctcttcaa tgacggaccg cacgccatgc tgctggattc gcttttcacc
     1141 ggcaacgact ggaaaacgat catcacgaat gagatactcg cctgtctcga tagcgcgaaa
     1201 ggtgtggaag gcaattgcag caacccgttc cttttttccg tgatgtgcag ggaacgtttt
     1261 ctcgtgacgc cggaacacct cgcgcttttc ccgccttcgt acatgaaaaa cggaacacag
     1321 ctggaagtgg atattccatg gagcaagctg tcgccttacc tgcggaaaga tgtcgcgtcg
     1381 aagatcgggg tacgtgattg aagccgggag gtttaaaagt ttctggtttc tggtttgaaa
     1441 aacaaacaac ggacaaaaat caggaaacaa ttcaagtcca aaatccgtgc ttaaaagcaa
     1501 cccctggctg ccagatcaag tcaaagctgc ttcaactaac gggcggcaat ctgtaaaggt
     1561 ctttcaaccc tgttctccct ttccagcctg atttcccgtc tccatttccc cgatcaccct
     1621 cttttccgtc cttctgacat ttccccgacc cattttccct catctttccc tatattcggg
     1681 ttcctaaaaa acaagcattc caatcatgaa aacagtgtca cggattatct ctcttgctgc
     1741 tgcggtcaca tttgcatttt cactgaacgc gcaggacaaa accaacaaaa aagacagcca
     1801 gatcaaattc accgtcgtat cggatgccaa atgcacgagt gtgaaggatc agtatcattc
     1861 aggtacgtgc tggtgctttt ccaccgaatc gttcctcgaa tccgaattca tgcgcatggg
     1921 caaagggccg cttgatcttt ccgaaatgtg gatcgtgcgc gccggctaca tcgagaaagc
     1981 gaaaaaatac atgcgcatga tgggcaaaac gaatttcggt cccggcggcg aacccggcga
     2041 cgtgatctcg ctctcggcgc aatacgggat cgtaccgcag gatgcttatc ccggtctgcc
     2101 cgaaggagaa acgaagatcc gccacggtga aatggacgat gtgctcaaag ccattctcga
     2161 cgcacagctc aagctcgagg acggaaaact ttctccgaac tggctcgcgg cttatacggc
     2221 tgcactcgac gcttacctcg gtgcgccccc ggcgaacttc accgtcaacg ggaaaagcta
     2281 cacaccgaaa tcatacgcgg atttcctcgg cttcaaaccg gaagattacg tggcgatcac
     2341 ttcgttcacg catcacccgt tctacacgca gttcccgctt gaaattcccg acaactggag
     2401 ctggagcccg tattacaacg ttccgctttc cgatcttact tccatcgccg acaacgccac
     2461 gatgaacggg tacaccgtcg catggggcgc tgacgtgagc gaaggttatt gctcgccgaa
     2521 gaacggcatg gcgatcgttc ccaacaaaaa ctgggacctg atgcgaaagg gagaaaaaga
     2581 ttcgctgtgg atcttacctt caaaagattc gatcatcacg caggatgtgc gccaggcggc
     2641 gtttgacaac cttagcacgc aggacgatca cggcatgcag ctc
//
LOCUS       DASTSF010000231        16599 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1818181,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000231 DASTSF010000000
VERSION     DASTSF010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 16599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16599
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1818181"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..380)
                     /locus_tag="VFU15_06720"
     CDS             complement(<1..380)
                     /locus_tag="VFU15_06720"
                     /inference="COORDINATES: protein motif:HMM:NF015427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PCRF domain-containing protein"
                     /protein_id="HEU4717505.1"
                     /translation="MFDIDGKLKKIAEEEVVSQAPGFWDDPKKAESLLKEIKKKKNWT
                     SSFTELEGSIEDLSVMYEFFKAGEASEQDLDNQYSETLKKTDDLEFRNMLSGREDHLG
                     AVLTINSGAGGTESCDWASMLMRM"
     gene            727..1203
                     /locus_tag="VFU15_06725"
     CDS             727..1203
                     /locus_tag="VFU15_06725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717506.1"
                     /translation="MNEPKHAHFILSDGQYCHILGDKLIIAKKEIPEKLPASSDSPDM
                     VSVALETGGMLILAFLLYMTILASAFTLSALISGLFLLLAFSLVRQFSFSNAKVILRA
                     NILGVEYHRRAIGYDYFIVIYSGEKGKLCRRRMAIYDSSQCLEQALISMKSEGLLK"
     gene            complement(1204..1683)
                     /locus_tag="VFU15_06730"
     CDS             complement(1204..1683)
                     /locus_tag="VFU15_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HEU4717507.1"
                     /translation="MENNSFKKRTEPSAALVKAEAWCAFQERCQQEARDKLYSWGLHR
                     DEVENIISELISRGFLNEERFARAYAGGKFRIKKWGRVKIMIELKRRKISDYCLRKAM
                     EEIDEDDYLRVLKKIIADKMRTEKEKNPLKKKLKVMRYAVSRGYENDLVRGIMEEDE"
     gene            1783..2496
                     /locus_tag="VFU15_06735"
     CDS             1783..2496
                     /locus_tag="VFU15_06735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717508.1"
                     /translation="MKRFFVFLLVLAALPAFAQSRTSNRNPGGQLSIGVRSTVSLFSG
                     AGGAGNGIGGQFRLRFYDFLNSEWFADYLNSSVGTLGTRTDYHIGWSVMFYLPGTVQK
                     YKHLKPIPYFLAGHCFDYTRIQGNNPFYETNSMASRWSSAVQMGIGMHIPLTPKIDFS
                     ASAQYMYHLGEEVFVETRTAVNGDQYLYVYKGAESGLEGHLLLTGSLNIRIADLWHDK
                     KGALGNGPHVDPDDSQQQQ"
     gene            2515..3198
                     /locus_tag="VFU15_06740"
     CDS             2515..3198
                     /locus_tag="VFU15_06740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717509.1"
                     /translation="MRKLILFLSLVFPAGFIFAQDPNQDTGDGPVTMNNLAGLLRTQL
                     TITPAWSVTAKSGNNSGSIAGGRTNVYLHGTVEYYFDERFSMRGDGYYFVNKMKTPGG
                     MKNNHTLEVGGSWHLWKGSMIDPFIGLGAGISYVQIQPADITNGAGLVLKNYAYPAHL
                     DPVWGPRAGVNFFSAKVFHFFVEAQYVMGSYRPSEGPILSLNEVRVSAGLGWNWVLLH
                     KEKTLRPTI"
     gene            complement(3232..5394)
                     /locus_tag="VFU15_06745"
     CDS             complement(3232..5394)
                     /locus_tag="VFU15_06745"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019464698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HEU4717510.1"
                     /translation="MKSALIITSLFAGFIIISCHTEAPPSSIPDVFADKVDSSANPAD
                     DFFQWANGKWIKNNPIPSSETHWGIGDLVQEEIYSNLRKISDDASKANAAEGTDTKKI
                     GDFWATAMDSVKADKAGLDPLKSQLAEINSIKTVDDAVRVSTDLDPFGADIFYSGFYV
                     MQDARNSEQMSITLEQGGLGLHERDYYFSGDSDDVKIRAAYVTHMRKMLELVGVPAPS
                     PDNVMNYVPDAPQDPHMAITTHSAQKKTPAMQQPADYKTSDKLAIGIMNFETALAGNS
                     RKLEDTRDPYENYNKMSMADFTKKYTPSFDWSAIAKAHGLQNADSVVVGQPEFFSSMD
                     KVLHATNIEVIKNYMIYHLVTAYAPYLSSSVTEENFDFYGKTMRGAKEQRPRWKRALD
                     SEEDAMGMVLGKLFAAAYFPEKAKKRYSDLVEAIRTAYGERIRKLTWMSDSTKAKALD
                     KLKRITKKVGYPDKWKDYSKLVVGTDSWCANMMYADEWEFNDMLSKYGKPVDRTEWDM
                     TPQTYNAYYNPSNNEIVLPAAIFTVPGYPDSLLDDAVVYGYAAASTIGHEITHGFDDE
                     GRNYDANGNLVNWWTAGDSASFVSRAAMMVHQFDAYEPLPGKNINGKATLGENIADYG
                     GLLLGIDAFKKTDQYKKGEKIGGYTPMQRYFMGYALGWLYSYRPESVAQQLMLDVHSP
                     AKFRVNGPFSDIPDFYDAFGVKQGDKMWRPDSLRVSIW"
     gene            complement(5509..6426)
                     /locus_tag="VFU15_06750"
     CDS             complement(5509..6426)
                     /locus_tag="VFU15_06750"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEU4717511.1"
                     /translation="MTSSIRKTTFLLAFLLSAALSFAQGSGSGDIKVSVTDEKNQPMP
                     GAIVHITAGGPNIGGQTDMDGVYTFRGLSPGTYSIEARMLGYKKYLKTGIIVNSGQTT
                     YPSFPMELSMCDTCVIEVVFQAGPIDKNYSTLHNISADEVKHMPVQKGDVIGMVTGTC
                     SSCSQGSSGGLVMRGAREGASSIYVDGEQLYGSQAVIGQSVQQVTILSGGIPAEYGDL
                     TGGAVIITTKSYYTGLAAKQSMYQDAADKKQAQQKADDAKSGKRVETNDQIIDKDDQN
                     NQNNNQNNNGNNNNGNNSQDKKQADPPKK"
     gene            6728..8152
                     /locus_tag="VFU15_06755"
     CDS             6728..8152
                     /locus_tag="VFU15_06755"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HEU4717512.1"
                     /translation="MKRTAIIGAGFTGTMLAVQLIRKATSPFELIIINERETFCKGIA
                     YNPYSKQHLLNVVAGKMSAFPDQPEHFLQWILRRPSFSGKDRELVANSFLPRFLYGEY
                     LSDTWNHTAKIAEEKNIRVRVIDGFVLDLDVFSDSVVLLTDNEEKIEVTDCIIATGNS
                     VPGNPRIPNLSFYDSPYYFRDPWQVDAVRGADGTKPVLIIGNGLTMVDTVTGLLGHGF
                     TNEIHAISPNGFNILPHRHNGLKYTRLVDELPESFSLYDIVRLVNRHIRSVRELGISA
                     EPVIDSIRSHTQRIWRALTEEEKKLFMSRLRHLWGVARHRIPLHVHDRLQQLRIDGQL
                     HVRSGTILDIKVKDNGAEVEYFDKKASVHRRINVSRVINCTGPETDLLKTENSFLKLS
                     MLKGLVSQDALKLGLRADVKSYRVIDASGNAHQHLFTLGPSLKGELWESTAVNELRVQ
                     AEELAERLCSESQQPAVYEKPVRK"
     gene            complement(8171..8812)
                     /locus_tag="VFU15_06760"
     CDS             complement(8171..8812)
                     /locus_tag="VFU15_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019597918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU4717513.1"
                     /translation="MQLKSFTFGPFYENTYVLWDETRECVIIDPGCYTDEEEDELRDF
                     IRDNGLAPVRLLNTHAHVDHVFGNPFVAKEWNLLPEMHREDLRVLHSFPQVCMLYGLN
                     GKEQPEPEKFLDEGQKITFGKTEMDIFFTPGHSPGSICFYNAKEKILLGGDVLFQGSI
                     GRTDLPGGDFETLMKSIRGKVFQLPPDVRVYSGHGPQTTIGEEKRSNPFVGEG"
     gene            9042..12410
                     /locus_tag="VFU15_06765"
     CDS             9042..12410
                     /locus_tag="VFU15_06765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717514.1"
                     /translation="MIYNRKFFLAWGILLLLFLPSLLDAQFYNGSQTDFGQNRVQYQD
                     FKWNYYKFDKYEVYYNTGGKELAIYTAKAAKKHIADIEKFFDYTLDDRIQFIVYNKQE
                     DFKQSNVGLTSDDGSSNVGGMTRIAGRKVFLYYEGDHIKFEEQIRAGIAEILINQMMY
                     GGNMRDMVKNSTLLNLPEWYIRGLIDYVAKGWNTDIDNRVNDGIMSGRFRKFNHLTGN
                     DALYAGHSIWYYISEKYSPAVIPNILYMTKVSRNVESSFLFVLGVNMRVLTMEWMNYY
                     TERYSMNDTTRYLPNYGPLIMKPKSSRVYSEAKISPDGRYITYVTNEMGQVKLWLEEL
                     STGKRKRLFKQGQKLDRINDYSFPLVAWHPSSKVLSFIYEHEGETWLEIYDVEAGDKT
                     VKPIYNFIKILDYSYSPDGKTFVMSAVANGQTDIYVYQIAGGGAVPVTKDIYDDLDPR
                     FVENGKSIIFSSNRTNDTLSHVDPDTKLNKSFDLFLYNNQTHSPVLRRITNTPGINEF
                     KAQPWDSTHFTFISDKNGIYNRFVGYFDSTIAYIDTSAHYRYLSRSIPVTNYKYSILD
                     QEVVPSANQLSEIIFHNGRYYIYNEPITPFASLTSSTLKNTAHRDRMIELAKIDAEID
                     SVEKQKNEVHPPEPGKPMQTVKVIDPGKKKDTTRIDINNYQFGGPDAPKKYDANVPPP
                     PKNTNVTGTDTTQRDTSVALKDQFVLPIARNYKVQFSTEYVVSQLDNSFLNAGYQRFT
                     GGGSPVYLNPGFTGLFKIGMTDLLEDHKIVGGVRLSGDLKSNEYVLYYEDRTHRWDKS
                     LMLHRQAFLNVNLNGGPITKIYTHEARYGLKYPFNEVLSFRTYMGYRNDRSVYLATDY
                     ANLLKGNTYANSANAKVELVFDNTIKRGLNLYNGCRFKIYAETFQGLDSLVKAQNGAN
                     RWFKNDLYDVGFDFRYYQKVHREIIWATRVAAATSFGHEKLVYYLGGVDNWLFPKFDQ
                     SINVATDQNYTYQTLATPMRGFWQNIRNGNSFAVINSELRVPLLRYLFNRPLRSDFFN
                     NFQVIGFGDLGTAWTGPDPYSERNSLNTTVISATGNPITVVLKNHTDPLVGGYGFGLR
                     SRLWGYFVRIDWAWGIEDRQHVPRVTYISFSLDF"
     gene            12417..13631
                     /locus_tag="VFU15_06770"
     CDS             12417..13631
                     /locus_tag="VFU15_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018343334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEU4717515.1"
                     /translation="MEATATLKTTVKEAAKTLLPEVKKIRRHLHRHPELSFREFETSA
                     FVAHELEKENIPFEKKTGTGIVALIEGKNPGKKIIALRADLDALPIQEKNETDYCSVN
                     AGVMHACGHDVHTASLLGAAKILRSLRDRFEGTVKLIFQPGEEKTPGGASLMIADGVL
                     ENPAPSEIFAQHVFPSLPAGKAGFRSGMYMASTDELYLAIKGKGGHAAMPQEYINPLL
                     IASSVLLKLQAQFMNEATKEKDAKEFPTVLAFGKIEGKGATNVIPETVTIEGTFRTMN
                     EAWRKKAHALIAETAKKTANELGGDAELRVEHGYPFLVNEETLTEKAKRAAEDFLGKE
                     NVVQLEQRMTAEDFAFYSQRIPACFYRLGTASRDGKYTSGVHTPTFDIDENALETGMG
                     LLAWIALDALGS"
     gene            complement(13663..15771)
                     /locus_tag="VFU15_06775"
     CDS             complement(13663..15771)
                     /locus_tag="VFU15_06775"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU4717516.1"
                     /translation="MTPRVLPLLFLLAFPILVSAQDQETIDSLTHVAKKNPPDTSSVR
                     AYLELSDATLNSDYDKSMSYAQSGLAIARLLKDDSWSGRCYYQMGTACFSYGLSMEAV
                     DHFTDAVAVLEKKDSAGYWVAKSMNGMGNAQRRLGRLKEATDSYLEAKRIFEKIGDRK
                     GVAGTYNNLGIILMAEGQQENALDYFKIARQMNIEINNQSWLAKNLSNMGTAFFNLQE
                     YDSAEVYFRQAFGIYLAVGDMNGWATDLINLGNVYTLKKEYAKAKEQFDLALDYDRRN
                     GREADMAFALLNMGGLYTETGDYRTAEKYLDSSMVISTKYNDLQTLIEVYKGYTVMYE
                     KSGDFKNAYKYYSLYATKKDSISSSDMKNQMDQMEESLREEKLREQNASLEQSKMLQD
                     VQLNRSHIISYTAFGGIALVLLLAFALFKRYQLKKKANDLLKERNREIQSQKTIIEEK
                     NRDISDSINYAKKIQDTVLPDISAFHSALGESFVYYRPRNVVSGDFYWFHKAGNKTFV
                     AVADCTGHGVPGAFMSMIGIDKMNQAVLEPGTLDPSVILTTVNRQLKTVLKQDDLSGG
                     MRDGMDVALISIDRGKMTLEFSGANRPVWITREGSLIELDPLKVSVGGHTPASQEFPL
                     QEFALQKNDCIYLFSDGYADQFGGPKEKKMMTRNFRQVLLSFSNEPMETQKKILSEKF
                     DAWKGSLEQVDDVCVAGIRV"
     gene            15966..16547
                     /locus_tag="VFU15_06780"
     CDS             15966..16547
                     /locus_tag="VFU15_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014680191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HEU4717517.1"
                     /translation="MEEFYTIQGEGFHSGKAAYFIRLGGCDVGCHWCDVKESWDANLH
                     PLTETDSIIRNASSFPSKAIVVTGGEPSAFNLSRLTSGAKKNGLQTFIETSGAYPLTG
                     EWDWICLSPKKTSPPLKENFKKAHELKVIIYNNDDFRWAEENALNVNSGCLLFLQPEW
                     SRAKQMMPLIVDYVMKHPQWNVSLQTHKFMNIP"
BASE COUNT         3835 a         4614 c         4241 g         3909 t
ORIGIN      
        1 tacatgcgca tgagcatgct ggcccagtcg cagctctcgg ttccgcccgc cccggaattg
       61 atcgtcaata cagcgccgag atgatcttcg cgtccgctga gcatgttgcg gaattcgaga
      121 tcgtccgttt ttttgagcgt ttcggaatac tggttatcga ggtcctgctc ggaagcttcc
      181 cccgctttga aaaattcgta catcaccgaa aggtcttcta tgctgccttc gagctcggtg
      241 aacgaggatg tccagttctt tttcttcttg atttccttca gcagcgattc agccttcttc
      301 ggatcgtccc agaaacccgg ggcctgcgat acgacttctt cttccgcaat ttttttcagc
      361 ttgccatcga tgtcaaagat gcctccttaa cgcatccttg cgatccacga gggccttgat
      421 ctgttctgtt gttatcatgg cgcaaatata accagagttt cgggttcgat gttccgtgtt
      481 ctgtgttcaa tgttccgcat gaaagacaaa gcatcgtggc cgttaccttc ttcttcctgc
      541 cgggcgttca tgctgcggtt cctgtgtaat tttcaacgca gtaccccgcg gctccgcctc
      601 tttgcgacgc gtttgttttg ctgcaaacgg attaagccac aaaaacgcag gcttccttcc
      661 aaaattatct tccgccggct ttgaaccccg gattttaaac acggaacgca atagtatctt
      721 tgccgcatga atgagccgaa gcacgcgcac tttattttat ccgacggaca gtattgccac
      781 attctcggcg acaagctgat cattgccaaa aaagagatcc ccgaaaagct gcccgcctcc
      841 tccgacagtc ctgacatggt gagcgttgcg ctggaaaccg gcggaatgct gatcctcgct
      901 ttcctgcttt acatgacgat ccttgcatcc gcattcacgc tgagcgcgct gatctcggga
      961 ctcttcctgc tgcttgcttt ttcgctggtg cggcaattca gtttttcgaa tgcgaaagtc
     1021 attctccgcg cgaacattct cggtgtggaa tatcaccgcc gcgcgatcgg ctacgattat
     1081 ttcatcgtga tctattccgg tgaaaaagga aagctgtgcc gccgccggat ggcgatctac
     1141 gattcttcgc aatgcctcga gcaggcgctc atttccatga aaagcgaagg tctcctgaaa
     1201 tgatcattcg tcctcttcca tgatcccgcg cacgaggtca ttttcatacc cgcgcgaaac
     1261 cgcgtaacgc atcactttca gctttttctt cagcgggttc ttttcttttt ccgtgcgcat
     1321 tttatccgcg atgatctttt tcagcacacg caggtaatcg tcttcatcga tctcctccat
     1381 tgctttccgc aggcagtaat cgctgatctt tctccgcttc agttcgatca tgatcttcac
     1441 tcttccccat ttttttatcc tgaatttccc ccccgcatac gcacgggcga aacgttcctc
     1501 gttcaggaaa ccgcgcgaga tcagctccga gatgatgttt tccacttcgt cgcggtgaag
     1561 tccccacgaa tagagtttgt ctctcgcctc ctgctggcac ctttcctgga atgcgcacca
     1621 cgcttccgct ttcacaagcg cagctgaagg ttcggtacgt tttttgaaag aattattttc
     1681 catcgcgcat actaaaatag ggaaagcaac cacaaagaaa ggacgacgtc attgggacaa
     1741 aaggagtttt attctatttt agttcctcgt aacaccctgt ttatgaaacg tttttttgtt
     1801 ttcctgctcg tgcttgccgc attgccggca ttcgcgcagt cacgcacatc caaccggaat
     1861 cccggcggac aattatcgat cggcgtgcgt tcaacggtca gtcttttcag cggcgcgggc
     1921 ggcgcaggca acggcatcgg cggacagttc cgtctccgct tctacgattt cctgaattcg
     1981 gaatggtttg ccgattacct gaactcgagt gtcggcacac tgggaacgcg aacggattat
     2041 cacatcggct ggtcggtgat gttctacctt cccggcaccg tgcaaaaata caagcacctg
     2101 aagcccatcc cgtattttct cgcgggacat tgcttcgatt acacacgcat ccagggcaac
     2161 aatccattct acgaaaccaa cagcatggct tcgcgatgga gctccgccgt gcagatgggc
     2221 atcgggatgc acattccgct gacaccgaag atcgattttt ccgcatcggc gcaatacatg
     2281 tatcacctcg gcgaggaagt tttcgtggag acccgcaccg ccgtcaacgg cgaccagtat
     2341 ctctatgttt acaaaggcgc cgaaagcggg ctggaaggac atcttctgct cacgggcagc
     2401 ctgaacatcc gcatcgcgga tctctggcac gataaaaaag gcgcgctcgg caacggtccg
     2461 cacgtcgatc ccgacgattc acaacaacag caataatcct ccattcccta ttttatgcgc
     2521 aagctgatcc ttttcctttc actggttttt cccgccggat ttattttcgc acaggacccg
     2581 aaccaggaca ccggcgacgg ccccgtcacc atgaacaatc tcgccggcct gctgcgcacg
     2641 cagctgacga tcacccctgc gtggagcgta acggcaaaat caggcaacaa ttccggttcg
     2701 atcgccggcg gacgcaccaa tgtctatctc cacggaacgg tggaatatta tttcgacgag
     2761 cgtttttcca tgcgcggcga cggctattac ttcgtgaata aaatgaagac gccgggcgga
     2821 atgaaaaaca accatacgct ggaagtcggc ggatcatggc atctctggaa aggaagcatg
     2881 atcgatccgt tcatcggtct cggcgcagga atttcctacg tgcagatcca gccggccgat
     2941 atcaccaacg gggcgggcct tgtgttaaaa aactacgcgt acccggctca cctcgacccg
     3001 gtatggggtc cgcgcgcggg cgtaaacttt ttcagtgcga aagtgtttca ctttttcgtg
     3061 gaagcgcaat acgtgatggg atcgtaccgt ccttctgaag gaccgatcct ttcactgaat
     3121 gaagtgcgcg tttccgcggg actcggatgg aactgggtgc tgctgcacaa agaaaaaacg
     3181 ctgcgcccca cgatctgagc acagcgttcc ttttgtctta caaatgtgat cttaccagat
     3241 gctcacacgc aggctgtcgg gacgccacat cttgtcgccc tgcttcacgc cgaacgcatc
     3301 gtagaaatcc gggatatccg agaacggccc gttcacgcgg aacttcgccg gcgaatgaac
     3361 gtcgagcata agctgctgcg ccaccgattc aggacgatag ctgtacaacc agccgagcgc
     3421 gtagcccatg aagtagcgct gcatcggtgt gtaaccgccg atcttttccc cttttttata
     3481 ctggtcggtt ttcttgaaag cgtcgatgcc gagcagaaga ccgccgtaat ccgcgatgtt
     3541 ctcaccgagc gtcgctttgc cgttgatgtt ttttcccggc agcggttcgt acgcatcgaa
     3601 ctggtgcacc atcatggcag cgcggctcac aaatgaggcg gaatcaccgg ccgtccacca
     3661 gttcaccagg tttccgttcg catcgtaatt tcttccttcg tcatcgaagc cgtgcgtgat
     3721 ctcgtgcccg atcgtggaag cggccgcgta accgtacaca acggcatcat cgagcagcga
     3781 atcgggatag cccggaaccg tgaagatcgc agcgggaaga acgatctcgt tgttcgacgg
     3841 gttgtaatat gcgttgtacg tctgtggcgt catgtcccac tcggtgcggt cgacgggctt
     3901 cccgtatttc gacagcatgt cgttgaattc ccactcgtcg gcgtacatca tgttcgcgca
     3961 ccaggaatcc gttccgacca cgagcttcga ataatctttc catttgtcgg gatagccgac
     4021 ctttttcgtg atgcgtttta atttatcgag cgctttcgcc ttggtgctgt cgctcatcca
     4081 tgtcagctta cggatgcgct ctccgtaagc cgtacgtatc gcttcgacga gatcgctgta
     4141 acgctttttc gctttttcag ggaaataagc agcggcgaat aattttccca gcaccattcc
     4201 catggcatct tcctcggagt ccagcgcgcg tttccaccgc ggacgctgct ctttcgcgcc
     4261 tcgcatcgtc ttcccgtaga aatcgaaatt ctcttcggtc accgacgagg acaggtaagg
     4321 cgcataagca gttaccaggt ggtagatcat gtaatttttg atcacttcga tattcgtggc
     4381 gtgaagaact ttatccatcg acgagaaaaa ttccggctgt cccaccacca ccgaatccgc
     4441 attctgcagt ccgtgcgctt tggcaatagc ggaccaatca aaagacggcg tgtatttctt
     4501 cgtgaaatcc gccatcgaca ttttgttgta attctcgtac ggatcgcggg tatcttcgag
     4561 cttgcgggaa ttcccggcaa gggccgtttc aaaattcatg atgccgatcg cgagcttgtc
     4621 cgacgttttg taatccgccg gctgctgcat agcaggtgtt tttttctgag cgctatgcgt
     4681 ggttatggcc atgtgcggat cctgcggtgc atcggggaca taattcatca cgttgtccgg
     4741 ggagggcgct ggaacgccaa cgagctcgag catcttgcgc atgtgcgtaa cgtacgctgc
     4801 gcgtatcttc acgtcatcgg aatcgccgct gaaataataa tcacgttcgt gcagaccgag
     4861 tccgccctgc tcgagggtga tggacatctg ttcgctgttg cgcgcgtcct gcatcacgta
     4921 aaacccggaa tagaaaatat ccgctccgaa cggatcgaga tcggtcgaca cgcgcacggc
     4981 gtcatcgaca gtcttgatgg aatttatttc cgccagctgg cttttcagcg ggtcgagccc
     5041 ggccttgtcc gctttcacgg aatccatggc ggtcgcccag aaatcgccga tcttcttcgt
     5101 atcggttcct tccgccgcgt tcgctttgga agcatcgtcg ctgatctttc tcaggttgga
     5161 ataaatttct tcctgtacga gatcaccgat cccccagtgc gtttcggagg aagggatggg
     5221 attattcttt atccattttc cattcgccca ctgaaagaaa tcatccgcgg ggttggcgga
     5281 agaatccacc ttgtcggcga aaacatcggg gatggaagag ggaggcgcct ccgtgtggca
     5341 ggagataata ataaaacctg caaagagcga agtgataata agagcggatt tcatgggctt
     5401 acgtttgtgg cgcaaattaa gaggaattag gatagaagcc ttaccgttca tcaaactgta
     5461 taaaaaaacc tcccgttccc gggaggttca ctcattcgca gcacggggtt atttctttgg
     5521 cggatcagcc tgtttcttgt cctggctgtt gttgccgttg ttattgttgc cgttgttatt
     5581 ctgattattg ttctggttat tctgatcatc cttgtcaatg atctggtcgt tcgtttccac
     5641 gcgtttcccg cttttcgcat cgtcggcttt ctgctgcgcc tgtttcttat cggccgcatc
     5701 ctggtacatg gattgttttg cggcgagacc tgtataataa cttttcgtcg taatgatcac
     5761 cgcgcccccg gtgagatcgc cgtattccgc cgggattccg ccggaaagga tcgtcacctg
     5821 ctgcacggat tgcccgatga ctgcctggga gccgtacaac tgttcgccgt cgacataaat
     5881 agagctggcg ccttcgcgcg ccccgcgcat aacgagtccg ccgctgctac cctggctgca
     5941 ggaagagcag gtcccggtca ccatcccgat cacgtcgcct ttctgcaccg gcatgtgctt
     6001 cacctcgtcg gcggaaatat tatgcagggt cgaatagttc ttgtcgatcg ggccggcctg
     6061 gaaaaccact tcgatcacgc acgtatcgca catggataat tccatgggaa atgacgggta
     6121 cgttgtttgt cctgagttga cgataatgcc ggtcttcagg tatttcttgt agcccagcat
     6181 gcgcgcttcg atcgaatacg tgccggggga aagtccgcgg aacgtataca cgccgtccat
     6241 gtccgtctgt ccgccgatat tcggaccacc ggccgttata tggacgatcg cgccgggcat
     6301 gggctggttt ttttcatcgg tgacgctcac tttgatgtcg cccgatccgc ttccctgcgc
     6361 aaaagacagc gcggccgaaa gaaggaacgc gagcaggaag gtcgtttttc tgatactgga
     6421 agtcatagta gtatgattta ggttgatcgt gcagtttgtt ttctgaataa ggaacgtgct
     6481 gacgcacgga attccacacg atcccgggcc ggatcatcag aaacggcgag cgccgggcaa
     6541 tatccggcgg caggagatgg ttaagtggcg aatgaatatt cctcccggaa ttttgcgaaa
     6601 ctgttcttcc tctgaaaaat tcttccccgc agggaaaggg cgaaatacct ctaattttaa
     6661 ccggaagaaa aattaaccgg tatcttgttc tggtgccggg atctttaccg gaaagaaaaa
     6721 aagacagatg aaacgtaccg caataatcgg cgcaggattc acgggaacaa tgctggcggt
     6781 ccagctgatc cgtaaagcca cctcaccatt cgaactcatc attatcaacg aaagggagac
     6841 cttctgtaag ggcattgctt ataatccgta ctcgaagcag catttgctca acgtggtggc
     6901 gggaaagatg agcgccttcc cggatcagcc ggaacatttt ctccaatgga tactccgccg
     6961 tccttctttt tcaggcaaag accgcgagct ggtggctaat tcttttctgc ctcgcttcct
     7021 ctatggagaa tacctttcgg atacgtggaa ccacactgcg aaaattgccg aagaaaaaaa
     7081 catacgtgtc cgggtaatcg atggtttcgt tctcgatctc gatgttttca gcgactcggt
     7141 cgtactgctg acggacaatg aagaaaaaat cgaagtgacg gactgcatca tcgctaccgg
     7201 caattccgtg cccgggaatc cgcggatccc gaatttgtct ttttacgaca gcccgtatta
     7261 tttccgtgat ccgtggcagg tggatgcggt gcgtggtgca gacggaacga aaccggtgct
     7321 tattatcggc aacggcctga ccatggtgga tacggtaacc gggctgctcg ggcacgggtt
     7381 cacaaatgag atacatgcca tttccccgaa cggcttcaat atcctgccgc accggcacaa
     7441 cgggctcaaa tacacccggc ttgtcgacga gctcccggaa tccttttcac tttatgatat
     7501 cgtccggctg gtgaaccggc acatcaggtc ggtgcgcgag ctgggcattt ccgcagaacc
     7561 tgtgatcgat tccattcgtt cgcatacaca gcggatatgg cgggccctca cggaagaaga
     7621 aaaaaaactg ttcatgtccc gtctccgtca cttgtggggt gtcgcgcggc accgcattcc
     7681 gctgcatgtc cacgatcgtc ttcagcagct gcgcatcgac ggacagctgc acgtgcgttc
     7741 cggaacgatc ctcgatataa aagtgaaaga caacggtgcg gaagtggaat atttcgacaa
     7801 gaaagcttcc gttcacaggc gcataaacgt gtcgcgcgtg atcaactgca caggtccgga
     7861 aaccgacctg ctaaaaacag aaaacagctt tctcaagctt tccatgctga aaggactggt
     7921 cagccaggat gcgctgaaac tcgggctgcg cgcagatgtg aaatcttacc gcgtgatcga
     7981 tgccagcggg aacgcacatc agcacctgtt cacgcttgga cccagcctta aaggtgaatt
     8041 atgggaaagc accgccgtca atgagcttcg cgtacaggcg gaggagctgg cggaacggct
     8101 gtgcagcgaa tcgcagcagc cggctgttta tgaaaaaccg gtcagaaaat aagaacggaa
     8161 gaggatccgc tcatccctcg ccgacaaagg gattgcttcg tttctcctca ccgatcgtcg
     8221 tttgcggacc gtggcccgaa taaaccctga catcgggcgg aagctggaac acttttccgc
     8281 gaatgctttt catcagcgtt tcaaaatcgc cgccgggcag gtccgtacgg ccgatgctgc
     8341 cctggaaaag cacgtcgccg cccaggagta ttttttcttt cgcgttgtaa aaacagatgc
     8401 ttcccggtga atgtcccggc gtgaaaaaaa tatccatctc cgtttttccg aacgtgatct
     8461 tttgtccttc gtcgagaaat ttttccggct ccggctgctc tttcccgttg agcccgtaca
     8521 gcatgcacac ctgcgggaac gaatgcagca cacgcagatc ttcccggtgc atttccggca
     8581 gcaggttcca ttccttcgcc acgaaaggat ttccgaaaac gtgatcgaca tgtgcatgcg
     8641 tgttcagcag gcgcacgggc gccaggccgt tgtcgcggat aaaatcgcgg agctcatcct
     8701 cttcttcatc tgtataacac ccgggatcga tgataacaca ttcgcgtgtt tcatcccaga
     8761 gtacatacgt gttctcgtaa aacgggccga aagtgaagga tttgagttgc atactgcaaa
     8821 ggtaagcagg gaacagcggg gagcgaatgc gggcagcagc cgctctgaaa atcggggaat
     8881 cccgcaggga cacaatgaat tgcaaccact gcgttgccgg aacgatggtt ttggctaact
     8941 ttattgtccg gttgaaatcc agccgggacc gaacggaaac ctccccacac ataaagttta
     9001 ccgtccggtt tttggagctt atgttttaca agttgtccgg catgatctac aaccgcaaat
     9061 ttttcctggc gtggggaatt ctcttgcttc tctttttgcc gtccttactg gatgcgcaat
     9121 tctataacgg ctcgcagacc gatttcggcc agaaccgcgt gcagtaccag gatttcaagt
     9181 ggaattacta caagttcgac aagtacgagg tgtattacaa caccggcgga aaagagctgg
     9241 cgatctacac ggcgaaagcc gcgaaaaagc acatcgccga catcgagaaa tttttcgatt
     9301 acacgctgga cgaccgcatc cagttcatcg tttacaacaa acaggaagat ttcaagcaga
     9361 gcaatgtcgg gctgacctcc gatgacggtt cttccaatgt gggcggaatg acgcgcatcg
     9421 cgggacggaa agtgttcctt tactacgaag gcgaccacat caagttcgaa gagcagatac
     9481 gcgcaggcat tgccgaaatc ctcatcaacc agatgatgta cggcgggaac atgcgcgaca
     9541 tggtgaagaa ttccacgctg ctcaacctgc cggaatggta catccgcggg ctgatcgatt
     9601 acgtggcgaa aggctggaat accgacatcg acaaccgcgt gaacgacggc atcatgagcg
     9661 gacgcttccg caagttcaac cacctcaccg gcaacgacgc gctctatgcc ggccattcga
     9721 tctggtacta catctcggaa aaatacagcc cggccgtcat cccgaacatt ttgtacatga
     9781 cgaaagtgag ccggaacgtg gaaagctctt tcctcttcgt gctcggcgtc aacatgcgcg
     9841 tgctgacaat ggaatggatg aactattaca cggaacgcta ttcgatgaac gacacgacgc
     9901 gttacctgcc gaattacgga ccgctcatca tgaaaccgaa atcctcgcgc gtgtacagcg
     9961 aagcgaagat cagtcccgac ggaaggtaca tcacgtacgt caccaacgaa atgggacagg
    10021 tgaagctgtg gctggaagaa ctttcgacgg gaaaacgcaa acgccttttc aagcagggac
    10081 agaagctcga ccgcatcaac gattattcat tcccgctcgt ggcgtggcac ccgagcagca
    10141 aagtgctttc gttcatttac gagcatgaag gagaaacgtg gctcgagatc tacgatgtgg
    10201 aagcgggcga caagacagtg aagcccattt acaacttcat caagatcctt gactattcct
    10261 attcgcccga cgggaaaaca ttcgtgatgt cggccgtggc gaacgggcag acggatattt
    10321 acgtttacca gatcgccggc ggcggagccg ttcccgtgac gaaagatatt tacgatgatc
    10381 tcgatccgcg cttcgtggaa aacgggaaga gcatcatttt ttcctcgaac cgcacgaacg
    10441 acacgctttc gcacgtcgac ccggatacga agctgaacaa atcgttcgac ctttttcttt
    10501 acaacaacca gacgcattca cctgttctcc ggcgcatcac caacacgccc ggcatcaatg
    10561 aattcaaggc gcagccatgg gactctacgc atttcacgtt catcagcgac aagaacggga
    10621 tctacaaccg cttcgtcggc tatttcgaca gcacgatcgc gtacatcgat acatccgcgc
    10681 attaccgcta tctctcgcgc agcatcccgg tcaccaatta caaatacagc atcctcgacc
    10741 aggaagtcgt tccttccgcg aaccagcttt ccgagatcat tttccacaac ggacgctatt
    10801 acatctataa cgaaccgatc acaccgttcg cttcgctgac ttcctccacg ctgaaaaaca
    10861 cggcgcaccg cgaccgcatg atcgagctgg cgaagatcga tgcagagatc gattcggtgg
    10921 aaaagcagaa gaacgaagtt cacccgcccg agcccggcaa gcccatgcag acggtgaaag
    10981 tgatcgatcc cggcaagaaa aaagacacga cgaggatcga catcaacaac taccagttcg
    11041 gcggacctga cgcacctaaa aagtacgacg cgaacgtgcc gccaccaccg aagaacacta
    11101 acgtcaccgg aacggacacg acccagcggg atacatcagt cgcgctgaaa gatcagttcg
    11161 tcctgccgat cgcgcgcaac tacaaagtgc agttcagcac cgagtatgtc gtttcgcagc
    11221 tggacaacag cttcctgaat gcaggttacc agcgtttcac cggcggagga agtcccgtat
    11281 acctgaatcc cggtttcacc gggttgttca agatcggcat gacggatctt ctcgaagacc
    11341 acaagatcgt gggcggcgtg cgtttgtcgg gtgatctgaa aagcaatgaa tacgtactgt
    11401 attacgaaga ccgtacgcat cgctgggaca aaagcctgat gcttcaccgc caggcgttcc
    11461 tgaatgtaaa tctcaatggc ggaccgatca cgaagatcta cacgcacgaa gcgcgctacg
    11521 gactgaaata cccgttcaac gaagtgctca gcttccgcac ctacatgggt taccgcaatg
    11581 accgcagcgt ttacctcgcc acggattacg cgaacctgct gaaaggaaat acgtacgcga
    11641 attcagcgaa cgcgaaagtg gagcttgtgt tcgacaacac gatcaagcgg ggactgaacc
    11701 tttacaacgg ctgccgtttc aagatctatg cggaaacatt ccagggactt gattcgctcg
    11761 tgaaagcgca gaacggcgcg aaccgctggt tcaaaaatga cctgtacgac gtgggattcg
    11821 atttccgcta ttaccagaaa gtgcaccgcg agatcatctg ggcgacgcgt gttgcggcag
    11881 ccacctcgtt cggtcatgaa aaacttgttt attatctcgg cggcgtcgac aactggctgt
    11941 tcccgaaatt cgaccagagc atcaacgtgg ccaccgacca gaattacacg taccagacgc
    12001 tcgccacacc gatgcgcggc ttctggcaga acatccgcaa cgggaacagc ttcgccgtca
    12061 tcaacagcga gctgcgcgtt cccctgctgc gctacctgtt caaccgcccg ctgcgttccg
    12121 atttcttcaa caatttccag gtcatcgggt tcggcgatct cggaacggcg tggacaggac
    12181 ccgatccgta cagcgaacgc aattcgctca acacgacggt catttccgcg acaggcaacc
    12241 cgatcacggt ggtgctgaaa aaccacaccg atccgctcgt cggcggttac ggtttcggat
    12301 tgcgctcacg cctgtggggt tattttgtcc gcatcgactg ggcgtggggc atcgaagacc
    12361 gccagcacgt tccgcgcgtt acatacattt ctttcagtct cgatttctga tccgttatgg
    12421 aggcgaccgc cacactgaaa accaccgtaa aggaggctgc aaaaacattg ctgcccgaag
    12481 taaaaaagat acgccggcac ctgcaccgcc accccgagct ttctttccgg gaattcgaaa
    12541 cgtccgcttt tgtcgcgcac gagctggaaa aggaaaatat cccgttcgaa aagaaaacag
    12601 gaaccggtat cgttgcgctg atcgaaggaa aaaatccggg taaaaagatc atcgcgctgc
    12661 gcgccgacct cgacgcactg cccatacagg aaaagaacga aacggattac tgttccgtga
    12721 acgccggcgt catgcatgcc tgcgggcacg atgtgcatac cgcttcgctg ctgggcgctg
    12781 caaaaattct ccgttcgctc cgcgaccgtt tcgaaggaac cgtgaagctg atctttcagc
    12841 ccggtgaaga aaaaacgccg ggcggcgcat cgctcatgat cgcggatggc gtgctggaaa
    12901 acccggctcc ttcggaaata ttcgcgcagc atgtttttcc ttcgctgcct gcggggaaag
    12961 ccggtttccg cagcggcatg tacatggcgt cgacggacga actttacctg gccatcaaag
    13021 ggaaaggtgg tcacgccgcg atgccgcagg agtacatcaa cccgctgctc atcgcttctt
    13081 ccgtcctgct gaaactgcag gcgcaattca tgaatgaagc gacgaaagaa aaagacgcga
    13141 aggaattccc taccgttctc gctttcggaa agatcgaagg gaaaggcgcc acgaacgtta
    13201 tccctgagac agtaaccatc gaaggcacat tccgcacgat gaacgaagcg tggcggaaaa
    13261 aagcgcatgc gctcattgcc gaaaccgcaa aaaaaaccgc gaatgagctg ggcggtgatg
    13321 cggaacttcg cgtggagcac ggctacccgt tcctcgtgaa tgaagaaacg ctcacggaaa
    13381 aagcgaaacg ggcggcggaa gattttctcg gcaaagagaa cgtcgtgcag ctggagcaac
    13441 ggatgaccgc ggaagatttt gctttttatt cgcagcggat acccgcgtgc ttctacaggc
    13501 tcggcaccgc atcacgcgac ggaaagtaca cttccggcgt tcacacaccc acgttcgaca
    13561 tcgacgagaa cgcactggaa acaggaatgg gattgctggc gtggatcgca ttggatgcgc
    13621 tcggaagctg aacggcaaat gatctcccgc cggcgcacat cactacacgc ggatcccggc
    13681 gacgcacaca tcatccacct gctcgaggct gcctttccac gcgtcgaatt tttccgagag
    13741 gatctttttt tgtgtctcca tcggctcgtt tgaaaacgaa agaagcacct gcctgaaatt
    13801 cctggtcatc attttcttct ctttcggtcc gccgaactga tctgcatagc cgtccgaaaa
    13861 aagataaatg caatcgttct tctgcagggc gaattcctgc aacggaaatt cctgcgaggc
    13921 aggcgtatgt ccgccgacgg aaactttcag tggatcgagc tcgatcaggc ttccttcccg
    13981 ggtgatccac accggccggt tggcaccgga aaattcgagc gtcatttttc cacggtcgat
    14041 ggagatcagc gccacatcca tgccgtcgcg cattcctccc gacagatcgt cctgcttgag
    14101 gactgttttc agctggcggt ttacggttgt caggatgacg gacgggtcca gcgtaccggg
    14161 ttcaagcacg gcctggttca tcttgtcaat gccgatcatg ctcatgaacg cgcccggaac
    14221 accgtggccc gtgcaatcgg cgactgcgac gaatgttttg ttgcccgcct tgtggaacca
    14281 gtagaaatcg ccgctcacca cattccgcgg gcggtaataa acaaacgact cccccagcgc
    14341 ggaatgaaag gcggatatat ccggcagaac ggtgtcctgt attttttttg cgtagttgat
    14401 gctgtcggag atgtcccggt tcttctcttc gatgatcgtt ttctgcgact ggatttcccg
    14461 gttacgttct ttcagcaggt cgttggcttt cttcttaagc tggtagcgtt tgaacagcgc
    14521 gaaagcaagc agcagcacga gagctatccc gccgaaagcc gtgtagctga tgatatgcga
    14581 gcggttgagc tggacatcct gcagcatttt tgactgttca agcgacgcat tctgctccct
    14641 gagcttttct tcccgcagcg attcctccat ctggtccatc tggttcttca tgtccgagct
    14701 gctgatggaa tctttcttcg tcgcatacag cgaataatat ttgtaggcat tcttaaagtc
    14761 acccgacttt tcatacatca ccgtatagcc tttgtatact tcgatgagcg tctgcaggtc
    14821 gttgtatttc gtgctgatga ccatgctcga atcaaggtat ttttccgccg tccggtaatc
    14881 tccggtttct gtgtaaagtc cgcccatatt cagcagggcg aatgccatgt ccgcttcacg
    14941 gccgttcctg cggtcatagt ccagcgcgag gtcgaactgt tccttcgctt ttgcatactc
    15001 cttcttcagc gtataaacat tcccgaggtt gatgaggtcg gtcgcccatc cgttcatgtc
    15061 accgacagcg aggtaaatcc cgaacgcctg cctgaaatac acttccgcag aatcgtattc
    15121 ctggagatta aaaaaagcgg tccccatgtt cgagagattt ttcgccagcc aggactggtt
    15181 attgatctcg atattcatct gccgcgcgat cttgaaatag tccagcgcat tttcctgctg
    15241 gccttccgcc atgagaatga tcccgagatt attgtacgtt cccgcaacgc ccttcctgtc
    15301 gccgatcttc tcgaaaattc tttttgcttc aaggtagctg tccgtcgcct ctttcagccg
    15361 cccgagccgc cgctgcgcat tacccattcc attcatgctt ttcgccaccc agtagccggc
    15421 gctgtccttt ttttcaagaa cggcaaccgc atctgtaaaa tggtctacgg cttccatgga
    15481 aagtccgtaa gaaaaacagg ctgttcccat ctggtaatag caccggcccg accaggaatc
    15541 atctttcagc aggcgggcaa tggccagccc gctctgcgca taactcatgc ttttatcata
    15601 atcggaattg agtgttgcgt ccgaaagctc caggtatgcc cggacggaag aagtgtccgg
    15661 cggatttttt ttcgcaacgt gcgtcaggct gtctattgtt tcctggtcct gtgcggaaac
    15721 aagaatggga aacgcgagaa ggaaaagcag gggtaaaacc cggggtgtca taacggaatt
    15781 tgatttccaa tttagctgaa aaagacttcc caaatgaagc accgctaaaa tgttcacaga
    15841 ccggaatttc cccgtgcttt gtctttacct ttgtgtctcc aatgaaattc accggcaacg
    15901 tcctcccttc caccaccagt tccgcgcagc aggaaaagct ggatgcagga gcgctcctgc
    15961 cgctcatgga agaattctac acgatccagg gcgaagggtt tcattcgggg aaagcggcct
    16021 atttcatccg cctcggcggc tgcgatgtcg gctgtcactg gtgcgatgtg aaggaaagct
    16081 gggacgcgaa cctgcacccg ctcacggaaa cggattccat tatccggaac gcttcgtcat
    16141 tcccgtccaa agccattgtc gtgacgggcg gcgaaccttc tgcgttcaac ctgtcccgtc
    16201 ttacgtcagg agcaaaaaag aacggactgc agacgttcat cgaaacctcc ggcgcctacc
    16261 cgcttaccgg ggaatgggac tggatctgcc tgtcaccgaa aaaaacaagc ccgccactga
    16321 aggaaaactt caaaaaggca cacgaattga aggttatcat ttacaataac gacgatttcc
    16381 gctgggcgga agaaaatgcg ctgaacgtga acagcggctg cctgcttttt cttcagccgg
    16441 aatggagccg cgcgaaacaa atgatgccgc tgatcgttga ttatgtaatg aagcacccgc
    16501 aatggaacgt ttcgttgcag acgcataaat tcatgaacat cccgtaaagg agtttgcagc
    16561 ttcccgttac cggttccctg tttctgcagc cggtaagcc
//
LOCUS       DASTSF010000232         2388 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_912110,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000232 DASTSF010000000
VERSION     DASTSF010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2388
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_912110"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /locus_tag="VFU15_06785"
     CDS             <1..224
                     /locus_tag="VFU15_06785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717518.1"
                     /translation="YPWAILSDGLAVLGLHQTTHFSHPTITFFAADMKEKIDQLKKDG
                     LENYSEQSPASITVITPEQQRINLFKMGM"
     gene            complement(250..2118)
                     /locus_tag="VFU15_06790"
     CDS             complement(250..2118)
                     /locus_tag="VFU15_06790"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU4717519.1"
                     /translation="MANFTELGLREELVSAVNDLGFTQPTPIQEQAIPLLITTPADLV
                     GLARTGTGKTAAFGLPLLNFIDVNAKIPQALVLCPTRELCMQISEDLKSFAKHMRGVY
                     ILPVYGGSSISMQIKDLKRGVQVVVGTPGRLIDLIERGALKLGEVQTVVLDEADEMLN
                     MGFRDDIDTILAETPAEKKTWLFSATMPEGVARIARKYMHDPVNVSVKGAHETDGQID
                     HIYCVVNARERYQSLRRVIDAHPDIYGIVFCRTKAETQEVADHLIRDGYTADALHGDL
                     SQAQRDFVMKRFRHRTIRMLVATDVAARGIDVDDITHVLHYQLPDEPESYTHRSGRTG
                     RAGKSGVSILFIHAREMGRLRETERRINKKFAYMPVPSGVDVLKNQVLSFANRIREGA
                     DVSVPHEYLSLFNQALGEMDTDALLQKIISFSLKDLFSKYENAPDLNAAVRPDKPEKF
                     TRHEERPTGEVRTFFFSLGRMDNVDPGVLLRICCDRMNVSKRAIGRIDLKHNFSFIQT
                     IGLPSAKVLDAFKNFSYQGRKVRVNEAENGEGERRGGESSGWGEKRGRKDYGERSFPV
                     KKEFRGKEKSSFTKQKSDFAPKKFHDDTKKNHDADSWQSLMKSEEGSDRKKKFKKK"
BASE COUNT          496 a          658 c          658 g          576 t
ORIGIN      
        1 cttatccctg ggcaatcctt tcggacgggc tcgctgtact cggtcttcat caaacgacac
       61 atttttctca tcccacaatc acctttttcg ccgcggacat gaaagaaaaa attgaccagc
      121 tgaaaaaaga cggcctggaa aattattcgg aacagagtcc tgccagcatc acggtgataa
      181 cgccggaaca gcaaaggatc aaccttttca agatgggaat gtagcgaagg aattgcgggg
      241 aaccggtatc tatttcttct tgaatttttt cttccggtcg ctcccttcct cgctcttcat
      301 cagcgactgc cagctgtcgg catcgtgatt tttcttcgtg tcgtcgtgga attttttcgg
      361 agcgaaatcg cttttctgtt ttgtgaagct gcttttttct ttcccgcgga actctttctt
      421 gacggggaat gaacgttcgc cgtaatcttt tcgcccgcgt ttttcgcccc acccggaact
      481 ttcaccgccg cggcgttcgc cttccccgtt ctccgcttcg ttcacgcgga ctttccgtcc
      541 ctggtaagaa aaatttttga acgcgtccag cacttttgcg gaaggaagac cgatggtctg
      601 gatgaaggaa aaattatgct tgaggtcgat gcggccgatc gcccgcttcg aaacgttcat
      661 gcggtcgcag cagatgcgca gcagcacgcc gggatcgacg ttgtccattc ttccgagact
      721 gaaaaagaac gtgcgcacct cgccggtggg acgttcttcg tgccgcgtga atttttccgg
      781 cttgtcggga cgcacagccg cattgagatc gggcgcgttc tcgtatttgg aaaaaagatc
      841 tttcagcgaa aaggaaatga tcttctgcag caacgcgtcg gtatccattt caccgagcgc
      901 ctggttgaaa agcgaaagat attcgtgcgg cacactgacg tccgctcctt cacggatgcg
      961 gttggcgaaa gaaagcacct ggttcttcag cacgtcgacg ccggaaggta ccggcatgta
     1021 ggcgaacttt ttgttgatcc ggcgttccgt ttcgcgaagc cgtcccatct cgcgtgcatg
     1081 aataaacagg atggaaacgc cgcttttgcc cgcgcggccg gtccgcccgc tgcggtgcgt
     1141 ataactttcc ggttcgtcgg gaagctggta atgcagcacg tgcgtgatgt cgtccacatc
     1201 gatgccgcgt gcggcgacgt cggtcgcaac gagcatgcgt atggtgcggt ggcggaaacg
     1261 tttcatcacg aaatcgcgct gcgcctggga aagatcgccg tgcaatgcgt cggccgtata
     1321 gccgtcgcgg atgagatggt ctgccacttc ctgcgtttcc gctttcgtgc ggcagaacac
     1381 gatgccgtaa atatccgggt gcgcatcgat gacgcggcgc agcgactggt aacgctcacg
     1441 cgcgttgacg acacaataaa tatgatcgat ctgcccgtcc gtttcgtgcg cgcctttcac
     1501 gctcacgttc accggatcat gcatgtattt gcgtgcgatg cgcgcaacgc cttccggcat
     1561 cgtggcggaa aacagccagg tttttttctc cgcaggtgtt tccgcaagaa tggtgtctat
     1621 gtcgtcacgg aaacccatgt tgagcatttc gtcggcttcg tcgagcacga cagtctgcac
     1681 ttcgcccagc ttgagcgcac cgcgttcgat caggtcgatg agacggcccg gggtcccgac
     1741 gaccacctgc acgccgcgtt tgaggtcctt gatctgcatg gaaatgctgg aacctccgta
     1801 aacgggaagg atataaacgc cgcgcatgtg cttcgcgaaa cttttcaggt cttcgctgat
     1861 ctgcatgcac agctcacgcg tgggacacag gacaagcgcc tgcggaattt ttgcattgac
     1921 gtcgatgaag ttcagcagcg gtagtccgaa cgcggctgtt tttcccgtgc ccgtgcgcgc
     1981 aaggccgacg agatcggcgg gcgtcgtaat aagaagcggg atcgcctgct cctgtatggg
     2041 cgtgggttgt gtaaaaccga gatcgttaac tgctgaaacg agctcttcgc gaagtccgag
     2101 ctctgtgaaa tttgccatta aggtgttgtt attaagctgc aaaggtaggc tttattgggg
     2161 cccgaaaaac ttccgtgccc cgcaagcccg tcagccgggc gatgttgcca tgcacgcgca
     2221 ggcatatctt cctcctgaaa actgcgaaga cgcaggacgc aaacggaaag caaaaacaaa
     2281 cctggtctta cctcgccagt atgatctttt tatccgcgct gcacgtttgt cccgtcacgc
     2341 gcacgagata aattccgtcc ggttgatcgg aaagatcgat ggacgccc
//
LOCUS       DASTSF010000233         3625 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_480396,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000233 DASTSF010000000
VERSION     DASTSF010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3625
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_480396"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..126)
                     /locus_tag="VFU15_06795"
     CDS             complement(<1..126)
                     /locus_tag="VFU15_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016656711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030527 - structural constituent of
                     chromatin [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEU4717520.1"
                     /translation="MNKAELIEAIANGSKLSKADAGRALDSTIESISKALKKGDKV"
     gene            complement(397..1362)
                     /gene="fmt"
                     /locus_tag="VFU15_06800"
     CDS             complement(397..1362)
                     /gene="fmt"
                     /locus_tag="VFU15_06800"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004294037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEU4717521.1"
                     /translation="MKRDPKSLRIVFMGTPGFAVGPLEMLVKEGYTVAGVVTVPDKPA
                     GRGKQLNESDVKKFALANGLKILQPEKLKAPEFIEELKALQPDLQVVVAFRMLPEIVW
                     SLPPLGTFNLHASLLPAYRGAAPINRAIMNGETKTGVTTFFLQQEIDTGSIVFREELS
                     IGENETAGELHDRLMIAGTALVKKTIDAIAEGDCPRIPQSSLVKTGETLPEAPKIFRD
                     DCRISWSSDAKKVHDHIRGLSPFPTAWTILKKENGEEIPLKIFRSEYFSGGTQQQPGE
                     IRNGLEVACGKGFVRVLELQAPGRKKLPAQEFLRGFPLGNNDRMI"
     gene            complement(1437..1766)
                     /locus_tag="VFU15_06805"
     CDS             complement(1437..1766)
                     /locus_tag="VFU15_06805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717522.1"
                     /translation="MNRNTRRLFIVPVAVLLLVACNLFHKTQKSEADYVSQGYVKAMV
                     TEIDLDGCKWMIRLDSTGKNLEPDELLPQFQKDSLQIWLKYKADDRMSVCMAGQTINV
                     LDIQLRK"
     gene            complement(1932..2285)
                     /gene="ssb"
                     /locus_tag="VFU15_06810"
     CDS             complement(1932..2285)
                     /gene="ssb"
                     /locus_tag="VFU15_06810"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEU4717523.1"
                     /translation="MTIQRNQVQLIGNLGMNPEVKSTTTGKKVARFTLANVSYKGKGD
                     EKSTGVNWFHLVAWEKQADIVEQYLYKGRRVGVSGRLVTRTWEDRDGKKHSLTEIVVN
                     DIVMMDEPKAETQAA"
     gene            2608..2979
                     /locus_tag="VFU15_06815"
     CDS             2608..2979
                     /locus_tag="VFU15_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU4717524.1"
                     /translation="MENLFNYVTKLDVKYKHLERIDVPEMVKENRHKWFNQTLTQVND
                     SVVRLGIVEGDYHWHKHDNDDEFFYVVDGQLLIEIEGDTIELNPGQGVTIPKGVMHRP
                     RAPKKTVMLMVETSGIIPTGD"
     gene            complement(3077..>3625)
                     /locus_tag="VFU15_06820"
     CDS             complement(3077..>3625)
                     /locus_tag="VFU15_06820"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEU4717525.1"
                     /translation="KPNQRGLYEHFSEVAQASPLPVILYNVPGRTSMNMTAETTLKLA
                     HDFPRIAAIKEASGNFEQIMAIIRDRPKNFKVISGDDLITLPLIAAGADGVISVVANA
                     FPKEFSEMVRLSLAGDYEKAGKLHYKLMRVTQLFFADGNPGGVKVALEAQKLCKAFVR
                     LPLFAVNDEVRSGILREVKKIR"
BASE COUNT          829 a          902 c          948 g          946 t
ORIGIN      
        1 gactttatcg ccttttttga gcgctttgct gatgctttcg atcgtggagt cgagcgcgcg
       61 acctgcatcc gctttggaga gcttagagcc gttggcgatt gcttcgataa gttctgcttt
      121 gttcattttg cgttggtttt atgtttaaca atgaatgagt ttgatgatga acaaatgtag
      181 tcgcattgaa ctatgaagca aaaatttctc ccgttaaaat gtgtgcattg ttcaaaactg
      241 catttttatg ttaataagtg cccctgaaac ccgctgcagc aaagggtttc agcgtatcga
      301 tgtcgtagaa cgccctgtgg cagagcgttt cacgaaaatt aaactgacaa atgtcaatga
      361 attccctcgc gaagcggcgt gcgatcatgc acgcattcag atcatgcggt cgttgttccc
      421 gagaggaaaa ccacggagaa attcctgggc cggaagtttt tttcttcccg gcgcctgcaa
      481 ttcgagtacg cggacaaaac cttttccgca ggcaacttcc agcccgttcc ggatttctcc
      541 cggttgttgt tgtgtgccgc cggaaaaata ttctgagcgg aatatcttca acgggatttc
      601 ctcgccgttc tcttttttca ggatggtcca cgcggtggga aaaggtgaaa gtccgcggat
      661 gtgatcgtgc actttttttg catcggagct ccagctgatg cggcaatcgt cgcggaaaat
      721 tttcggcgct tcgggaagcg tttcgcctgt ttttaccaat gaagattgcg gaatgcgcgg
      781 acaatctcct tcggcgatcg cgtcgattgt ttttttgacc agtgcggttc cagcgatcat
      841 cagccggtcg tgaagctcac ctgccgtttc attttcgccg atggagagct cttccctgaa
      901 aacgatgctg cccgtgtcaa tctcctgctg cagaaaaaat gtagtgacac cggtttttgt
      961 ttcgccgttc atgatcgcgc ggttgatcgg tgctgcaccg cgataagcgg gcaggaggga
     1021 agcgtgaagg ttgaatgttc ccagcggcgg cagcgaccag acgatttccg gcagcatgcg
     1081 gaacgccacc acgacctgca gatcgggctg cagcgctttc agctcttcga taaattcagg
     1141 cgctttcagt ttttccggct ggaggatctt cagtccgttc gcaagcgcaa attttttcac
     1201 gtcgctttcg ttcagctgct ttccgcgtcc cgccggcttg tccggaacag tgaccacacc
     1261 ggcgaccgtg tagccttcct tcaccagcat ttccagcggt cccaccgcga aacccggtgt
     1321 tcccataaaa acaatacgaa gtgatttcgg atcgcgcttc attcgttcgg ttcggtttaa
     1381 aggaaatatg cgcgcaaaaa tagcacgggg aaattatagt gctgaatttt cagaagttat
     1441 ttccgcagct ggatgtcgag cacattgatc gtctgtcccg ccatgcacac gctcatgcgg
     1501 tcgtcggctt tgtatttgag ccagatctga aggctgtctt tctggaattg cggaagaagc
     1561 tcgtcgggtt cgagattttt acccgtgctg tcgaggcgga tcatccattt gcagccgtcg
     1621 agatcgatct cggtcaccat ggctttcacg tatccctgcg atacataatc cgcttccgat
     1681 ttctgcgtct tgtgaaaaag gttgcaggcg accagaagaa gaacggcgac cggaacaatg
     1741 aaaagcctgc gtgtattcct gttcatgggt tggatttttt tcaaaagtaa ggattccacc
     1801 cattgctgca ggtgcgtccc ggttcgttca cccgccgggc tatgttcatg caaagatcgc
     1861 atgccccggt gcggataaaa aaaaggacca ccctttgcag agtgatcctt tcggccgcca
     1921 gatcgtaacg gtcacgcagc ctgcgtttcc gctttcggct cgtccatcat tacgatgtca
     1981 ttcacgacga tctccgtaag cgaatgtttc tttccgtcgc ggtcttccca tgtacgggtg
     2041 acgaggcgtc cgctcacacc gacgcggcgg cctttgtaga ggtattgctc gacgatatcc
     2101 gcctgctttt cccaggcgac aaggtggaac cagttcacgc cggtggattt ttcatcgcct
     2161 ttgcctttgt agctgacgtt ggcgagggtg aaacgcgcta ctttttttcc tgtcgtggtg
     2221 gatttgactt ccgggttcat gccgagattt ccgatgagct gcacctggtt acgttgaatg
     2281 gtcataaaaa ataaaattaa gagtgaaaca aaaaatgaat tgtacttcgc ttgcatattg
     2341 atgccgcttt tcgtggccac tttgaaagaa cgacggtgca aagatgtgag cctgtttttc
     2401 ccggacccgg ttgataaacg gatctatccg gatataagcg tttgcaaacg aatgcaactt
     2461 gtttcggccg ggtggcttgt attgctgaaa gaaaggaaaa gaggaaataa gttcagaaaa
     2521 ggcaggacag gatcgttctg aatgtgcgga attgttgtaa attacttttc gggattttgc
     2581 ttttacattt cataaaacac acacgccatg gaaaacttat ttaattacgt tacgaagctc
     2641 gacgttaaat acaagcacct cgaacgcatc gacgttccgg aaatggtgaa agagaacagg
     2701 cacaaatggt tcaaccagac gctcacgcag gtgaacgaca gcgtggtccg tctcgggatc
     2761 gtggaaggcg attaccactg gcacaagcac gataacgacg atgaattctt ttacgtggtg
     2821 gatggacagc tgctgatcga gatcgaaggc gatacgatcg agctgaatcc cggccagggt
     2881 gtaaccattc ccaaaggcgt catgcatcgc ccgcgcgcgc cgaagaaaac ggtgatgctt
     2941 atggtggaaa caagcggcat cattccgacg ggggattagc gttttgcggt tggaaaaagt
     3001 ttcccgttaa aacgcgagga acacgaagtg ttttgtaaaa aaactttgta cgctttgtcg
     3061 caggaaaaaa gaattcctac ctgatcttct tcacctcgcg cagtatcccg ctcctcactt
     3121 catcgttcac agcgaacaag ggcaggcgaa caaatgcttt gcaaagcttc tgcgcttcca
     3181 gcgccacctt cacgccgccg ggatttccat cggcgaagaa aagctgcgtc acgcgcatga
     3241 gcttgtaatg aagctttcct gctttttcat aatcaccggc cagtgacagc cgcaccattt
     3301 ctgaaaattc tttcgggaat gcattcgcca cgaccgagat gacgccgtcc gcaccggcag
     3361 caatgagcgg gagcgtgata agatcgtcgc cggaaatgac tttgaaattt ttcgggcggt
     3421 cccggatgat cgccatgatc tgctcgaaat ttcctgacgc ttccttgatc gcagcgatgc
     3481 gcggaaaatc atgcgccagc ttcagcgtcg tttccgccgt catgttcatc gacgtgcgtc
     3541 ccggaacgtt gtacaggatc acgggcaggg gagaagcctg cgccacttcg ctgaaatgtt
     3601 cgtagagtcc gcgctggttc ggctt
//
LOCUS       DASTSF010000234         2651 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1288858,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000234 DASTSF010000000
VERSION     DASTSF010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2651
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1288858"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1449)
                     /locus_tag="VFU15_06825"
     CDS             complement(<1..1449)
                     /locus_tag="VFU15_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEU4717526.1"
                     /translation="MENLYTRHKISISFVLGVILLAGIFAYTRIQTSLFPEITFPKIK
                     IIADNGQEPVEKMMITVTKPLENAVKQVPGLNMIQSSTSRGSCEISAYFNWNADINLC
                     QQQMESRISDIRSTLPPGLDFTVEKMNPAVLAVMGYTIEAPGKTPIEQKLIATNIVKP
                     FLSQVDGVSSIRVLGGKNKEYRAELNPQMMSSLSVTPEDVKNAFDHTAFIQSNGYSYD
                     YNRLYLNLTDAGIYSKTDLGNVVIRNDGKRVILLKDIAAIDIAEQVEYTRVNANGHEA
                     VLVNVFKQPDANLLQVSGDIEKKVTELNKILPPGVSLKPYYVQAHFVDDSIRSVEDSL
                     WIGLLLALIVSVIFLRSFRASLVVLITIPVTLALTLIILFALHYTFNIMTLGAIAAAT
                     GLIIDDAIVVIEQIHRAKEESPVNNVSSLISGSVKYLFPAMIGSSLSTIVIFVPFALM
                     GGVAGAYFHVLTNTMIITLTSSFFVTWIGLPVI"
     gene            complement(1451..2380)
                     /locus_tag="VFU15_06830"
     CDS             complement(1451..2380)
                     /locus_tag="VFU15_06830"
                     /inference="COORDINATES: protein motif:HMM:NF024829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEU4717527.1"
                     /translation="MKKYFPPVLFFLAVIFFAAGCSAPAADEEEDSAANVVTPVNVTH
                     VLSGPISSITELSAVTMFQKKISVRAPVSGYISEVDVNPGSTVREGQALFSVKTKEAS
                     ALDRQHVHDSLLGFPGDISIAAQKAGTITSLSHQKGDFVQEGDELAVISDASSLVFLL
                     QVPFEMRTFIKTGMDCSIVLPGGQELPGKITGSLPEVDAASQTQQFIVKANSTQPIPE
                     NLVARVRIVKSTKQNATIIPKTAVLTDETQSEFWVMKLISDSVAVKTIITKGIENRDT
                     VEILSPAFTKDDLLVTEGNYGLPDTAKVSVRNR"
     gene            complement(2382..>2651)
                     /locus_tag="VFU15_06835"
     CDS             complement(2382..>2651)
                     /locus_tag="VFU15_06835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717528.1"
                     /translation="LFFSAKQRMEISSLNFQLGEENRLIAQEQKTLGLSQSLMDGDKA
                     LLDQGQASVTDFILAVKNNIDLHHQLNISQVRKLEIINELNYWNW"
BASE COUNT          582 a          683 c          736 g          650 t
ORIGIN      
        1 gatcacggga agtccgatcc acgtgacgaa gaaagaactt gtcagcgtga tgatcatcgt
       61 gttcgtcagc acatgaaaat aagcgccggc cacaccgccc atcagcgcga acggaacgaa
      121 gatgacaatc gtgctcaggg aagaaccgat catggcgggg aagaggtatt tcaccgagcc
      181 ggaaatgagc gatgaaacat tattcaccgg tgattcttct tttgcacggt ggatctgttc
      241 gatcacgaca atggcatcgt cgatgatgag accggtggcg gccgcgatgg cgccaagcgt
      301 catgatgttg aacgtgtaat gcagtgcgaa caggatgatg agcgtgagcg cgagcgtaac
      361 gggaatggtg atgagaacaa caaggctcgc ccggaaagaa cgcaggaaga tcacggaaac
      421 gatcagcgca agcagcaggc cgatccagag cgaatcttcc acgctgcgga tggaatcatc
      481 cacgaaatgc gcctgcacgt aatacggttt cagcgaaacg ccgggcggaa gtattttatt
      541 aagctcggtt actttttttt cgatgtcacc ggaaacctgc agcaggttcg catccggctg
      601 cttgaacaca ttgacgagga ccgcttcatg cccgttcgcg ttcacacgcg tgtattccac
      661 ctgttccgcg atgtcgatgg cagcgatgtc tttcagcagg atcacgcgtt tcccgtcgtt
      721 ccggatgacg acattcccga gatcggtctt gctgtagata cccgcatccg taaggttgag
      781 gtaaagacgg ttgtaatcgt atgaatagcc gttcgattgt atgaaagcag tatgatcgaa
      841 agcatttttc acatcttccg gcgtaacgga aagcgacgac atcatctgcg ggttcagttc
      901 tgcacggtac tctttgttct ttccgccgag aacgcggatg gaagacacgc cgtccacctg
      961 cgaaaggaac ggcttgacga tgttcgtggc gatcagtttc tgctcgatag gggtttttcc
     1021 cggcgcttcg atcgtataac ccatcacggc cagcacagcg ggattcattt tttcgacggt
     1081 gaaatccagg cccggcggaa gtgtgctgcg gatgtcggag atgcgcgatt ccatctgctg
     1141 ctggcagaga ttgatgtcgg cgttccagtt gaaataggcg gagatctcgc agctgccgcg
     1201 gctcgtgctg ctctggatca tgttcagccc gggcacctgc ttcacggcat tttccagcgg
     1261 cttcgtcacg gtgatcatca ttttctccac cggctcctgc ccgttgtccg cgatgatctt
     1321 gatcttcggg aacgtgatct cgggaaaaag cgacgtttgt atccgcgtgt atgcgaagat
     1381 gcctgcgagg agaataacgc ccagcacgaa cgagatagag atcttatgtc gcgtatacag
     1441 gttttccatt ttaccggttg cggacgctga cttttgccgt atcgggaaga ccgtaattcc
     1501 cttctgtcac gagaagatca tcctttgtga atgctggcga aagaatttcc actgtgtcgc
     1561 ggttttcgat gccttttgtg atgattgttt ttacggccac gctgtcgctg atcagcttca
     1621 tcacccagaa ttccgattgc gtttcatccg tcagcaccgc agttttggga atgatcgtcg
     1681 cattctgctt cgtgcttttc acgatgcgca cccgggcgac aagattttca ggaatgggtt
     1741 gcgtggaatt ggctttgaca atgaactgct gtgtctgcga tgctgcgtcc acttccggaa
     1801 gcgagccggt tatttttccc gggagttctt gtccgccggg caaaacgatc gagcagtcca
     1861 tccccgtctt tatgaatgtc cgcatctcga acggaacctg cagcaggaac acgaggctgg
     1921 atgcatccga gatcacggcc agctcgtcgc cttcctgcac gaaatcgcct ttctggtgtg
     1981 aaagcgacgt gatggttccc gccttctgtg cggcgatgga aatatcgcca ggaaaaccga
     2041 gcagcgaatc gtgcacgtgc tggcggtcga gcgcggaagc ttctttcgtt ttaacgctga
     2101 aaagagcctg cccttcgcgc acggtgctgc cgggattcac gtcgacttcg ctgatgtaac
     2161 cggaaaccgg cgcccgcacg gatatttttt tctggaacat cgtcactgcg ctgagctcgg
     2221 tgatcgacga gatcgggcct gacagaacgt gcgtgacgtt taccggcgtg acgacattcg
     2281 ctgcgctgtc ttcttcctcg tcagccgcag gcgcgctgca acccgccgcg aaaaaaatga
     2341 cggcaaggaa aaagagaacg ggagggaaat attttttcat tttaccagtt ccagtaattc
     2401 agttcattga tgatctccag ctttcttacc tgcgaaatgt tcagctggtg atgaaggtcg
     2461 atattgttct tcaccgccag tataaaatcc gtgacggaag cctgcccctg gtcgagaagt
     2521 gccttgtctc catccatcag cgattgcgaa agtccgagcg ttttttgttc ctgtgcgatg
     2581 agccggttct cttcacccag ctggaaattc aggctgctga tctccatccg ctgttttgcg
     2641 ctgaagaata g
//
LOCUS       DASTSF010000235         8554 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_33561,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000235 DASTSF010000000
VERSION     DASTSF010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 8554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8554
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33561"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(412..1956)
                     /locus_tag="VFU15_06840"
     CDS             complement(412..1956)
                     /locus_tag="VFU15_06840"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010516496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HEU4717529.1"
                     /translation="MANQSDNFQDIISHSKEYGFIFQSSEIYENGLSAVYDYGQNGAE
                     LKKNIREYWWKSMVQLHENIVGIDAAIFMHPTTWKASGHVDAFNDPLVDNKDSKKRYR
                     ADVLIEDAVAKMEDKINKEVEKAAKRFGEKFDAAMFRSTNPRVVEYQKKIDDTNARFK
                     EAMVGSDMEAMRQIIVDLEIVCPVSGTRNWTDVRQFNLMFATQLGSVAEEASTIYLRP
                     ETAQGIFVNFLNVQKTGRMKIPFGIAQTGKAFRNEIVARQFIFRMREFEQMEMQFFIR
                     PGTEDEWYEKWKSMRMNWHRALGFPEDKYRFHDHVKLAHYAKAACDIEFAFPFGFKEL
                     EGIHSRSDFDLKQHEQYSGKKLQYFDPELGESYVPYVIETSIGLDRMFLATLSQSYKE
                     EKLEDGSERVVLNLPPFLAPVKAAVLPLVNKDGLPEKAQEIMDALKFDHNVSSEEKDS
                     IGKRYRRQDAIGTPYCITVDHQTLEDNTVTIRERDSMKQERVAIEKVPAIIDERVNVK
                     SVLRKL"
     gene            complement(1986..2927)
                     /locus_tag="VFU15_06845"
     CDS             complement(1986..2927)
                     /locus_tag="VFU15_06845"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEU4717530.1"
                     /translation="MKTVLGYFKTYYREEFRWPVFLAVLLFLTGTTWLFYTQKPVAAL
                     IGGGAAGSFRFFGNLLVFGVSFAVPLLIYAAAGLHRDKLRSPGFLLMAAVALLVFCFR
                     VFFYWERPMAEYFSGDSHTSYWQNCLTNVTSAFLLVVPVFVFWLCTDRKTQPFYGISA
                     GRVNWKPFVIFLAIMLPLILFAAHQPDFRAQYPKAAISLKRGGLPADDMGHALFFEGC
                     YGLDFIATEFFFRGFLVIGLAKFLGRSAILPAAVFYTFIHFGKPLGETISSFAGGLAL
                     GIIAYETKSVKSGLILHLGIAWMIEAVMLMHNGAGLF"
     gene            3064..3591
                     /locus_tag="VFU15_06850"
     CDS             3064..3591
                     /locus_tag="VFU15_06850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717531.1"
                     /translation="MFLLRLRPVVMLLLIVLPLAAGAVLQAAADLAGKNTQLQLASTA
                     LSFVFAMIFCAWIAALTWKLARGTLRRLVLLFLLLCGVVFRAWQDCWSVNKFLATGKM
                     PSVEEIDPLSAIFILHALVSLFVVYLLVWLSVSLVKKEKSSGISSSPLWQTLLSFFVF
                     PVGVFFIQRRIQRLS"
     gene            complement(3713..5038)
                     /locus_tag="VFU15_06855"
     CDS             complement(3713..5038)
                     /locus_tag="VFU15_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008507167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HEU4717532.1"
                     /translation="MKKIPAPFFALLIFCVSAASAQTDSTEKWSWHYQFTGVMQGHAA
                     FHAPYAGQNSLDSTAEQAFSVTSTFYFGRRLWTGGELYFNPEIAGGKGISSALGIAGF
                     TNGECFRIGDPSPALYVARVFVRQQFGLGDEKEWLDAGANQLAGWVPAKRITLTVGKV
                     SLADIYDANSYSHDPRTQFMNWSLMSNGAWDYAANTRGYTWAGMAEWHTPVFSARYCF
                     AMMPKVANGSVFDMNIGKANEEAIELEKSWGKDGSKTTVRVLGFYNMSHARSYQDAIL
                     QYHLGNDTTLDVNTQTTYGGKKYGFGINAETQLFNGKAGVFVRGGWNDGKTATWVFTE
                     IDMNSQVGIQLSGSLWKRSDDVFGLASSVNEISKDHQDFLNTGGYGFIIGDGKLPDYA
                     PEVILETYYSAQITDNFWLSADYQHVQNPAYNADRGPVNVWAVRGHVAF"
     gene            5428..>8554
                     /locus_tag="VFU15_06860"
     CDS             5428..>8554
                     /locus_tag="VFU15_06860"
                     /inference="COORDINATES: protein motif:HMM:NF019136.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HEU4717533.1"
                     /translation="MLIRGIIPSLAFLLFISCGEKKEARTGEWKPAITFSPGHRIDPD
                     TVPAPLIVTAGEPKTVPAGTLAAVPAGLNVMPAGEPVVVAAGKPQVIIPGTDTFPLPA
                     TARAGTPKITPAGPPETVEEMTARGDDNPGNFITYKALETETQNTVMCTTTDAGGNVW
                     FGTFGGVTFYDGRRFRNFSMTEGLPNNFVFSICAGKDSSIWIGTGLGGVCRFDGKSFT
                     TYTKKEGLPNNMVRSIFQDTHGNTWFGTSAGACRFDGKSFTTFTAKNGLAANHVYDIM
                     EDARGKIWIATTRGVSCYDGKTFSNYSTAAGLPDDTVFCLLQDRSGKIWFGTPAGACC
                     FDGKNFCSYTQKQGLINNRVESVMQDFSGRIWFGQENGISIFDGSTFTSFGKKQGMDG
                     NYARSMKQDRTGDTWIATMNGICHFDGNFFTGLTEKEGLPERTVRNFYRDREGRVWMA
                     TMDSGACFYDGKTFTRFTEKNGLTDNRVFVIHEDAKGNTWFGTSTGICFYDGTSVHRF
                     TQPQQGKGFSTKEGLSFNAVMSIAEDDSGRTWFGLFGGGLLRYDGKTFTKFSQKQGLV
                     SNNIISLERNPDGSLWIGTTNGASRYDGKTFTNFTAKDGLASDFIYCIAHDQDGITWF
                     GTSGGLTRYDGKSFMNFTTKDGLASPAVMSILPARDGNLWFGTREGLSCLPANRRKMI
                     GRFPAGFDPAKDVLFVNYGYNDGFSGLKCISNSVMQDSAGKIWWGADLLMIYDPAGEM
                     PDTLAPVLVINGIDLYGERVRWEQMGAVTMDTTGKETVGGIAKDTIIGHGIALSGIRF
                     DGITQWNELPVHLSLPYNNNNLTFRFTGVHLKDCSHVKYQYRLDGMDPDWSTITDRTE
                     APYGNLPPGTYTFELKAMNRSGVWSRPFTYSFEVRPPWWETWWFRISAGLLLLSSVAG
                     FFRYRTAALRKDKERLEKTVKERTAEVVKQKEVIEEKQKDILDSINYAQRIQRALLAS
                     DALLDENLHSHFVFFQPKDIVSGDFYWAAKLDNGNFALVTGDSTGHGVPGAIMSMLNI
                     SCLEKSIEQEKL"
BASE COUNT         2000 a         2330 c         2293 g         1931 t
ORIGIN      
        1 gttttgaatt ctcaatgata agaaaaaatg cagcttcggt tcccggtgaa ctgttcccgg
       61 aggtggtaac acgggaaaga aaaccgcggg agtgcagaga cgcgaaggaa tccgctcacg
      121 ggagcgggga gcgtgaaaac acaaactgcg cggctatttt tttgtcgcgg agatgccggg
      181 aagaattaat cacggagacg cggaaagcaa atgtgccgca cctcatcctt tttcatgccg
      241 ccttccacgc attagtattt tctattttct tccgcagcat attttcctcc gcggaagaca
      301 ccggatggaa aaaacaggaa taaaaaagac cggccgccgg atcgtgtgac aggcggtgat
      361 aattaccttt tcagaaaggc acaggtaaat tctccgggat aaagcgctcc tttacaattt
      421 ccgcagcacg cttttcacat tcacgcgctc gtcaataatg gcggggactt tttcgatggc
      481 gacgcgttcc tgcttcatgc tgtcgcgctc gcggatcgta acggtattgt cttccagcgt
      541 ctggtgatcg acggtgatgc aatacggcgt accgatcgca tcctggcggc ggtaacgttt
      601 gccgatggag tctttttctt ccgaggaaac gttgtgatcg aacttcagcg catccatgat
      661 ctcctgcgct ttttcaggaa gaccgtcttt gttcaccagt ggcagcacag ccgctttcac
      721 cggcgcgagg aacggcggaa ggttcagcac aacgcgttcg ctgccgtctt ccagcttttc
      781 ttctttgtac gactgcgaca acgttgcgag gaacatgcgg tcgaggccga tggacgtttc
      841 gatgacatac ggcacatagc tttcgcccag ctcgggatcg aagtactgga gttttttccc
      901 ggaatactgt tcgtgctgct tcaggtcgaa atcgctgcgc gagtgaattc cttcgagctc
      961 tttgaacccg aacgggaatg caaattcgat gtcgcacgcc gctttcgcat aatgcgcgag
     1021 cttcacgtgg tcgtggaaac ggtatttgtc ttcgggaaaa cccagcgcgc ggtgccagtt
     1081 catgcgcatg gatttccatt tttcatacca ttcgtcttct gttccggggc ggatgaaaaa
     1141 ctgcatttcc atctgctcga actcgcgcat gcggaaaata aattggcggg cgacaatctc
     1201 gttgcggaat gcttttcccg tctgtgcaat gccgaacgga attttcatcc ttcctgtttt
     1261 ctgcacattg aggaaattca cgaaaatgcc ctgcgctgtt tccgggcgta aataaatagt
     1321 gctcgcctct tcagccacag aaccgagctg cgtggcgaac atgaggttga actgccgcac
     1381 gtcggtccag ttgcgcgtgc ccgaaacggg acaaacaatc tcgaggtcga cgatgatctg
     1441 ccgcatggct tccatgtcgc tgcccaccat cgcttcctta aaacgcgcat tggtgtcgtc
     1501 gatctttttc tggtattcga ccacgcgggg attggtggaa cggaacatcg ccgcgtcgaa
     1561 tttttcaccg aaacgtttcg ctgctttctc gacttcctta ttgatcttgt cttccatttt
     1621 cgccaccgca tcttcgatga gcacatcggc gcggtagcgt ttttttgaat ctttattatc
     1681 aaccagcgga tcattgaacg catccacgtg accggatgct ttccacgtgg tgggatgcat
     1741 gaagatggcg gcgtcgatgc ccacgatatt ttcgtgcagc tgcaccatcg atttccacca
     1801 gtattcgcgg atgttctttt tcagctccgc gccgttctgc ccgtaatcgt acactgcgct
     1861 gaggccgttt tcgtagatct cgctggattg aaaaatgaac ccgtattctt tcgaatggga
     1921 aataatatcc tggaagttgt ccgactggtt tgccataagt gtgcaaagat aatgcggcga
     1981 atgaattaaa acaggcccgc gccattgtgc atcagcatca ccgcttcgat catccacgcg
     2041 ataccgagat gcaggatcag gccgcttttc accgacttcg tttcataggc gataatgccc
     2101 agcgcaagtc cgcccgcaaa cgagctgatg gtttcaccca gcggcttgcc gaaatggatg
     2161 aacgtgtaaa acaccgcggc gggaaggatt gcgctccgcc ccaggaattt cgccaggccg
     2221 ataacgagga aacccctgaa aaaaaattcc gtcgcgatga aatcaaggcc gtagcagcct
     2281 tcgaaaaaca acgcgtggcc catgtcgtcc gcaggcagcc cgccacgttt cagggaaatg
     2341 gccgccttgg gatattgcgc gcgaaaatcg ggctggtgcg cagcgaacag gataagcggc
     2401 agcatgatgg ctaaaaaaat cacgaacggc ttccagttca cacgcccggc gcttatgccg
     2461 taaaacggct gcgtcttccg gtcggtgcac agccagaaaa caaatacagg tacgacaagt
     2521 aaaaacgcag atgtgacgtt cgtgaggcag ttctgccagt acgacgtatg cgaatcgccg
     2581 gaaaaatatt ccgccatcgg ccgttcccag taaaaaaaca cgcggaagca gaacaccagc
     2641 agggccacag cagccatcag caggaagccc ggcgaacgaa gcttgtcgcg gtgaaggccg
     2701 gccgcggcgt agatcagcag cgggaccgcg aacgacacgc cgaagacgag aaggttgccg
     2761 aagaaacgga agcttcccgc agcaccgccg ccgatgagcg ccgctaccgg cttctgcgtg
     2821 tagaacagcc aggtggtgcc ggtgagaaaa agaagaacgg cgagaaaaac cggccagcgg
     2881 aattcttccc tgtaatatgt tttgaagtac cctaaaacag ttttcatcgg ggtggcggaa
     2941 attctccgtg accgggcgaa gatacaacta tgtaaaaacg ccggccgctt cgtttcagaa
     3001 tgagaatttg cgcttccgca gggatgatcc ggcgaaacgt tttcacttca ctaccttcgc
     3061 cgtatgtttc ttctccgcct gcgcccggtt gttatgctgc tgcttatcgt gctccctctc
     3121 gcagccggtg cagttctgca ggctgccgct gatcttgccg ggaagaacac acagcttcag
     3181 ctggcgtcaa ctgcgctctc atttgtcttc gcgatgattt tctgcgcgtg gatcgcggcg
     3241 ctgacctgga agctggcccg gggaactttg cgcaggctcg tgctgctttt cctgctgctg
     3301 tgcggagttg tattccgcgc gtggcaggac tgctggtcgg tgaataaatt tcttgctacg
     3361 ggaaaaatgc cttcggtcga agagatcgat ccgctttcgg ccattttcat tcttcacgcg
     3421 ctggtctctc ttttcgtggt ttacctgctg gtgtggcttt ccgtttcgct ggtaaaaaaa
     3481 gagaagtcgt cggggatttc ttcttctccc ttgtggcaga ccctgctttc cttcttcgtt
     3541 ttcccggtgg gcgtgttttt tatccagcga cgtattcagc ggttatcctg aatgatttat
     3601 cccggcgaag agcagcagca ggataaagta gataatgatc atcggcagaa agaaaatgga
     3661 acggggcggg gcacaccaat attacaactt tcggcgaagc tgtatctctc cttcagaatg
     3721 cgacgtgacc gcgcacggcc cacacgttca cgggaccccg gtcggcgttg taggcaggat
     3781 tctgcacgtg ctggtaatcg gcggaaagcc agaaattgtc cgtgatctgc gcggaataat
     3841 acgtttcgag tatcacttcc ggcgcataat ccggcagctt cccgtcgccg atgataaaac
     3901 catatccgcc ggtattgaga aaatcctggt ggtcttttga aatttcattg accgaagaag
     3961 cgagaccgaa aacatcatcg ctgcgtttcc acagcgaacc gctgagctgt atgccgacct
     4021 gcgaattcat atcgatctcg gtaaataccc acgtcgcggt tttcccgtcg ttccatcccc
     4081 cgcggacgaa aacgcccgct ttcccgttga acagctgcgt ttccgcattt atcccgaagc
     4141 cgtatttttt tccgccgtac gtcgtctgcg tgttcacgtc gagcgtggtg tcgttgccga
     4201 gatggtattg caatatcgca tcctggtagc tgcgcgcatg cgacatgtta taaaaaccga
     4261 gcacgcgcac ggttgtcttt gagccgtctt ttccccagct tttttccagc tcgatcgctt
     4321 cttcattcgc tttgccgatg ttcatatcaa acacggaacc gttcgccact ttcggcatca
     4381 tcgcaaaaca gtagcgtgcg gaaaaaaccg gcgtgtgcca ttcggccatt cccgcccacg
     4441 tatagccgcg cgtgttcgcc gcgtaatccc acgccccgtt gctcatcagc gaccagttca
     4501 tgaattgcgt acgcggatca tggctgtatg aatttgcgtc gtaaatatcc gccagcgaaa
     4561 cttttcccac cgtcagcgtg atgcgtttcg ccggcaccca tcctgcgagc tggttcgcgc
     4621 ccgcatccag ccattctttc tcgtccccga gcccgaactg ctgccgcaca aaaacacgcg
     4681 ccacgtacag cgcgggagaa ggatccccga tgcggaaaca ttctccgttg gtaaaccctg
     4741 caatgcccag cgcgctgctg atgccttttc cgccggcgat ctccgggttg aaataaagtt
     4801 caccgcctgt ccacaaacgg cgcccgaaat aaaacgtcga cgtgacggaa aacgcctgct
     4861 ccgccgttga gtcgagactg ttctgcccgg catacggcgc atggaaagcc gcatgcccct
     4921 gcatgacgcc ggtgaactgg taatgccagc tccatttttc ggtggaatcg gtttgtgcgg
     4981 aagccgcgga aacgcagaaa atgaggagag caaaaaaagg cgcggggatt tttttcatac
     5041 ggaaattttg aacaaatata ttttattttt tgtctggcaa taactttttt ttagtcatgt
     5101 ctaaattttg gagcaaagcc cggaacaggg aagcgggccc tgtggtacat gccggaaaaa
     5161 aggggggcgt cctcgctggt tgcggtgatt tttcatcccg gaagggtgga gaggcgcggc
     5221 gcaatgatac ggtgattttt gtcaattttt gtggggcgcc ttcttttctt ttataacggg
     5281 tccgcccatc ctgcaacacc tcaaattccc gatatttgaa aacagacctg cattcatgaa
     5341 acccctttcc tgctttcatt tcccgttgca acaggctccg ggccggcaac cggagagcag
     5401 ccgtttttcc cgggacatct ataccgtatg ctgatccgcg gaatcatccc atcgcttgcc
     5461 tttctgctct tcatttcgtg cggggaaaaa aaggaagcgc gcaccggaga atggaaaccg
     5521 gccatcacct tttccccggg tcaccgcatc gacccggaca ccgttcctgc cccgcttatt
     5581 gttacggccg gtgagccgaa aactgttccc gcaggaactc ttgccgctgt tcccgcaggt
     5641 ctgaacgtta tgcctgcagg agaacccgtg gttgttgcag caggaaaacc gcaggtgatc
     5701 attcccggca ccgatacgtt tccgcttccg gctaccgcgc gcgccggaac gccgaagatc
     5761 acgcctgccg gtccgccgga aacagtggaa gaaatgaccg cccgcgggga cgacaatccc
     5821 ggaaacttca tcacctataa agcgctcgag acggaaacgc aaaataccgt tatgtgcacc
     5881 accacggatg caggaggaaa tgtctggttc gggactttcg gcggcgttac cttttacgat
     5941 ggaagaaggt tccggaactt ttccatgacc gaaggattgc cgaacaattt cgtattcagc
     6001 atctgcgcag gaaaggacag cagcatctgg atcggaaccg ggctcggcgg ggtctgtcgt
     6061 ttcgacggaa aatcgttcac gacgtacacg aaaaaagaag ggcttccgaa caacatggtg
     6121 agatccattt tccaggacac ccatggcaac acttggttcg gcacttccgc cggagcctgc
     6181 cgtttcgacg ggaaatcatt caccactttc actgcgaaaa acggtcttgc cgccaaccat
     6241 gtctatgaca tcatggagga tgcgcgcgga aaaatatgga tcgccaccac gcggggcgtg
     6301 agctgctacg acggaaaaac attctccaac tacagcacgg ctgccggcct tccggacgac
     6361 actgtattct gcctgctgca ggaccgctcg gggaaaatat ggttcggcac acccgcaggc
     6421 gcctgctgtt tcgatggaaa aaatttctgc agctacacgc agaagcaggg cctgataaat
     6481 aaccgtgtag agtccgtcat gcaggatttc agcggcagga tctggttcgg gcaggaaaac
     6541 gggatcagca ttttcgacgg aagcacgttt acctcgttcg gtaaaaaaca gggaatggat
     6601 ggaaactatg cacgaagcat gaagcaggac cgaaccggcg acacatggat tgccacgatg
     6661 aacggcattt gtcacttcga cgggaatttt ttcacgggac ttaccgaaaa agaaggattg
     6721 ccggaacgaa ccgtacggaa tttttaccgc gaccgggaag gacgcgtgtg gatggcgacg
     6781 atggacagcg gagcctgttt ttacgacgga aaaacattca cgcgcttcac cgaaaagaac
     6841 gggctcaccg acaaccgcgt tttcgtcata cacgaagatg cgaaaggcaa tacatggttc
     6901 ggaacgtcca ccggtatctg tttttacgac ggaacatccg tgcaccgctt cacacagccg
     6961 cagcagggaa aaggtttctc gacgaaggaa ggcctcagct tcaacgcggt tatgagcatt
     7021 gcggaagatg acagcggacg aacctggttc ggcctgttcg gcggcggact tctgcgttac
     7081 gacgggaaaa cattcacgaa attttcgcag aagcagggac tcgtcagcaa caacatcatt
     7141 tccctggaaa ggaatcccga cggatcgctg tggatcggca caacgaacgg tgccagccgc
     7201 tatgacggga aaaccttcac gaatttcacg gcaaaagacg ggctcgcttc tgattttatc
     7261 tattgcatcg cgcatgacca ggacggaatt acgtggttcg gcacctccgg cggactgacg
     7321 cgctacgacg gaaaatcgtt catgaatttc acaacgaaag acggcctcgc cagtcccgcc
     7381 gttatgagca tcctgcccgc ccgtgacgga aacctgtggt tcggaacaag agaaggattg
     7441 agctgcctgc cggcgaaccg gcgaaagatg atcggccgtt ttcctgccgg cttcgatccg
     7501 gcgaaagatg tgctcttcgt caattacggg tacaacgacg gtttcagcgg actgaaatgc
     7561 atcagcaatt ccgttatgca ggatagcgcg ggaaagatct ggtggggcgc cgacctgctg
     7621 atgatctacg atcctgccgg cgaaatgccc gatacgctgg cccccgttct ggtcatcaac
     7681 ggtattgatc tttacggaga acgcgtacgc tgggaacaga tgggcgccgt gacaatggac
     7741 accaccggca aagaaaccgt aggcgggatt gcaaaggaca ccatcatcgg gcacggcatc
     7801 gcgttgtcag gcatccggtt cgacggtatc acacagtgga atgaactgcc cgttcacctg
     7861 agcctgccgt acaacaataa caacctcacc ttccgcttca ccggtgtaca cctgaaagat
     7921 tgcagtcacg tgaaatacca gtaccgcctc gacgggatgg atcccgactg gagcacgata
     7981 acggaccgca cggaagcacc ctatggcaac ctcccgcccg gaacatacac gttcgagctg
     8041 aaagccatga accgcagtgg cgtctggagc cggccgttca cgtattcatt cgaagtacgt
     8101 ccgccgtggt gggaaacgtg gtggttcaga atttccgccg ggcttcttct cctctcttcc
     8161 gtcgccggtt tttttcgtta ccgcacagct gccctgcgaa aagacaagga acgtctcgag
     8221 aaaacagtga aggagcgtac agcggaagtt gtgaaacaaa aagaagtcat cgaggaaaaa
     8281 cagaaagata ttctcgacag catcaattac gcgcagcgca ttcagcgtgc gctgctcgcg
     8341 agcgacgcgc tgctggacga aaatcttcac agtcattttg ttttcttcca gccgaaagac
     8401 atcgtgagcg gcgatttcta ctgggcggca aaactggaca acgggaattt cgcgctggtc
     8461 acaggcgaca gcacagggca cggcgtaccc ggggcgatca tgagcatgct gaatatttcc
     8521 tgccttgaaa aatccatcga gcaggaaaaa ttat
//
LOCUS       DASTSF010000236         3251 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_120180,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000236 DASTSF010000000
VERSION     DASTSF010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3251
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120180"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..643)
                     /locus_tag="VFU15_06865"
     CDS             complement(<1..643)
                     /locus_tag="VFU15_06865"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU4717534.1"
                     /translation="MKKTILLPALLLVPGMIFSQNKISGNVKDASTGEALPGVLLYVS
                     GTYSACASDADGNFQIINVKGNSAELIAMMIGYQRDTVHVDLPQTQPLNILLKQDTTV
                     LEDVIIRSTRVLHSEQGAYTDIKKEELHKNDFGQDLPFLLNQQPSVVTTSDAGAGVGY
                     TGIRIRGSDATRVNVTINGIPLNDAESQGTYWVDLPDFVSSTDNIQVQRGVGTS"
     regulatory      complement(696..824)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(862..1401)
                     /locus_tag="VFU15_06870"
     CDS             complement(862..1401)
                     /locus_tag="VFU15_06870"
                     /inference="COORDINATES: protein motif:HMM:NF024911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU4717535.1"
                     /translation="MEKNNAIRRIAILGAESTGKTTLARSLALRFSTAWIPEYAREYM
                     AEHPGAYTTDVVVEIAQKQLEEENRLVRQANKFLFVDTEFIIAKVWCEDVFGQCPEWI
                     AQQITGHRYDLYLLTSNDLPWIPDPVRVNPQRRDYFFNIYKNELEANQLPFEIVTGRY
                     EARLLHAIKIIERYFGSEE"
     gene            complement(1371..2024)
                     /gene="pnuC"
                     /locus_tag="VFU15_06875"
     CDS             complement(1371..2024)
                     /gene="pnuC"
                     /locus_tag="VFU15_06875"
                     /inference="COORDINATES: protein motif:HMM:TIGR01528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HEU4717536.1"
                     /translation="MKDSTGVLFSVFSSIRQTGFPELIAIITALIYVVLAARANKWCW
                     IFGIVSSAIYVWFFFVLTYYYDAFLNVYYVVIGFYGWYAWTRKKAETEESLPIYSADK
                     RTLLLLIATGAAATLLLGWGGPRLASFFAEIFHADVPPFKRPYVDAMLTSFSLVATWM
                     TTRKMIENWIFWVVIDAGYVIVCLISERPSTALLMFIYTIIAIFGYFRWKKTMPSGA"
     gene            complement(2021..2617)
                     /locus_tag="VFU15_06880"
     CDS             complement(2021..2617)
                     /locus_tag="VFU15_06880"
                     /inference="COORDINATES: protein motif:HMM:NF014622.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEU4717537.1"
                     /translation="MEKEAEVVTSLFEHGLGLFHLRKLSMRTNEMKEYLEAIPAHFHE
                     RIVLHSHHTLSLQYNVGGIHLTRYHRRKKLVNWLQLRWIRLRRPAIHTSLTFHKLADV
                     YRNNEKAEYAFLGTIYDKISGKFNSGYNRQSIEAAVKSSVIPLFARGGTSADNIALTH
                     ELGFAGMAFYSAIWKNQDPVKGYCDIAEKCRALNIPLE"
     gene            complement(2666..>3251)
                     /locus_tag="VFU15_06885"
     CDS             complement(2666..>3251)
                     /locus_tag="VFU15_06885"
                     /inference="COORDINATES: protein motif:HMM:TIGR01768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="geranylgeranylglyceryl/heptaprenylglyceryl
                     phosphate synthase"
                     /protein_id="HEU4717538.1"
                     /translation="FLVGGSLLDENTVESCILRIKKKSDVPVLIFPGHTMQLSAAADG
                     ILLLSVISGRNAELLIGRHVAGASLLQKSGIGIIPTGYILVDGGAPTSVNYMSQTFPV
                     PRDKPAIAAMTALAGEQLGLGAIYLEAGSGAQKPVPCEMISVVRKTVGVPLFVGGGIT
                     SRKEIDERFAAGADVVVIGNHLEKNPGDLQRFAE"
BASE COUNT          738 a          891 c          847 g          775 t
ORIGIN      
        1 tggacgttcc cacaccgcgc tgcacctgaa tgttgtcggt ggaagaaacg aaatcgggaa
       61 gatcaaccca gtacgttccc tgcgattcgg cgtcgttcag cgggatgccg ttgatggtga
      121 cattcacgcg cgtcgcatcg gaaccgcgga tgcgtatgcc cgtgtagccc acacctgcgc
      181 ccgcatcgga cgtggtgaca accgaaggct gctggttgag caggaacgga aggtcctgcc
      241 cgaaatcgtt tttgtgcagc tcttcctttt tgatgtcggt gtaagcgccc tgttcactgt
      301 gcagcacgcg cgtcgaacgg atgatcacgt cctcgagcac ggtggtatcc tgcttcagga
      361 ggatgttcag cggttgcgtc tgcggaagat cgacgtgcac cgtatcgcgc tggtaaccga
      421 tcatcatggc gatcagctcc gccgaattcc ccttcacgtt gatgatctgg aaatttccgt
      481 ccgcatcgga agcacaggca gaataagttc cgctgacgta cagcagcaca cccgggagcg
      541 cttctcccgt ggatgcatct ttcacgtttc ccgagatctt gttctgggaa aagatcatgc
      601 ccggcacaag tagtagtgcg ggcagcaaaa ttgttttttt catttgattt gtccgttgcg
      661 atccacagtt tcatacgaca agggacaatt cgcattatcc tgtggatcct gtttccctac
      721 gcaagtatta ccttgatcag gtgtgcggaa attgttctgg agaaacgaat ttccgccggg
      781 tatgatctca gccacgccgc agcatggcac cccttgagga tcggagcaaa agtaaaaagt
      841 atttgataac gcgcgctttt attattcctc gcttccgaaa taacgctcga taatcttgat
      901 cgcgtggagc aatctcgctt cataacgtcc cgtcacgatc tcgaacggaa gctggttcgc
      961 ctccagctcg ttcttgtaga tattgaaaaa ataatcccgc cgctgcggat ttacacgcac
     1021 cgggtcgggg atccagggaa gatcgttcga cgtcagcagg taaagatcgt agcggtgccc
     1081 cgtgatctgc tgcgcgatcc attcggggca ctgaccgaac acatcctcgc accacacttt
     1141 cgcgatgatg aattccgtgt cgacaaaaag aaacttattc gcctggcgga cgagccggtt
     1201 ttcttcctcg agctgcttct gcgcaatttc aacaaccacg tccgtcgtat aggcgcccgg
     1261 gtgttctgcc atatattctc gcgcatactc cggtatccac gccgtggaaa agcgcagcgc
     1321 aagcgatcgt gcgagcgttg ttttccccgt gctttccgca cccagtatcg ctatgcgccg
     1381 gatggcattg tttttttcca tctgaagtag ccaaagatcg caatgatcgt gtaaatgaac
     1441 ataagcagcg cggtggaagg ccgctccgag atcaggcaaa cgataacgta gcctgcatcg
     1501 atcaccaccc agaagatcca gttctcgatc attttccgcg tcgtcatcca cgtggcgacc
     1561 agggaaaatg aagtgagcat cgcgtcgaca tacgggcgtt tgaacggagg aacatcagca
     1621 tgaaaaattt ccgcaaaaaa tgaagccagc cgcggacctc cccatccgag aagaagcgtt
     1681 gcagcggctc ctgtcgcgat gagcagcagc agtgttcttt tgtccgcgga ataaatggga
     1741 aggctttctt ctgtttccgc tttttttctt gtccatgcgt accatccgta aaacccgatc
     1801 accacgtaat aaacgttcag gaaagcatcg taataatacg tgagcacgaa aaaaaaccag
     1861 acgtaaatgg cggagctgac gatgccgaat atccagcacc atttattcgc acgcgccgca
     1921 agcacaacat agatgagcgc cgtgatgatc gcgatgagct ccgggaaacc cgtttgacgt
     1981 atggaagaaa aaacggaaaa taaaacgccg gtgctgtctt tcattcaagc ggaatgttca
     2041 gtgccctgca tttttcagcg atatcgcagt accccttcac cgggtcctgg tttttccaga
     2101 tcgcactgta aaaagccatg cccgcaaaac ccagttcatg cgtcagcgcg atattgtctg
     2161 cagatgtgcc gccgcgcgca aagagaggga tcaccgagct cttcactgcc gcctcgatgc
     2221 tctgccggtt gtatcctgaa ttgaatttgc cgctgatctt gtcatagatc gtgccgagaa
     2281 aagcgtattc cgctttttcg ttgttcctgt acacatccgc cagcttgtga aaagtcagcg
     2341 acgtatggat cgccggccgc cgcaggcgga tccacctcag ctgaagccag ttcaccagtt
     2401 tcttacgccg gtggtaacgc gtgaggtgta tgcctcccac gttgtactgc agcgacagcg
     2461 tgtggtggct gtgaaggacg atgcgttcat gaaaatgggc agggatcgct tcgaggtatt
     2521 ctttcatttc attcgtccgc atggaaagtt tccggagatg aaagagaccg agtccgtgct
     2581 caaagagcga cgtcacgacc tccgcttcct tttccatggt tcttgtattt gaaaaaacga
     2641 cgactttcat gaggggcgtg tttttttatt cggcgaaacg ctgcaggtcc ccgggatttt
     2701 tttcaaggtg attcccgatc accaccacat ctgctcccgc agcgaaacgt tcatcgatct
     2761 ctttccgcga agttattcct cctcccacaa aaagcggaac accgactgtt tttcgtacca
     2821 cgctgatcat ttcgcaggga acaggtttct gtgcgccgct tccggcttcg agatagatcg
     2881 ccccgagccc gagctgctca ccggcaagcg cggtcattgc tgcaatggcg ggtttgtcgc
     2941 gcggaacggg gaaggtctgg ctcatgtaat tgaccgatgt tggcgctcct ccgtcgacga
     3001 gaatgtaccc ggtgggaatg atcccgatac ccgacttctg caggagtgag gcgccggcca
     3061 cgtgtctccc gatcagcagc tctgcatttc ttccggaaat aaccgacagc agcagaattc
     3121 cgtccgccgc tgcggaaagc tgcatggtgt gacccgggaa aatgagcacc gggacatcgc
     3181 ttttcttttt tatccgcagg atgcagcttt cgaccgtatt ctcatccagc aggcttccgc
     3241 cgacaagaaa a
//
LOCUS       DASTSF010000237         2734 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_122986,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000237 DASTSF010000000
VERSION     DASTSF010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2734)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2734
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122986"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..881
                     /locus_tag="VFU15_06890"
     CDS             <1..881
                     /locus_tag="VFU15_06890"
                     /inference="COORDINATES: protein motif:HMM:NF024806.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU4717539.1"
                     /translation="VLFLKFGFRSKRLALFGMAWFIVVLALPVWYGVTNGSGEQYEHR
                     VYTASPGAFLFLLQLKLPEAYRKTAQRFALLMIVFFVVKTSFRLPVYHDEFVYAKAGT
                     EEAPSTSFFHDMLGFMYQQKNQCTDALSCYNRAIALDSGNAEYYNHRGSVYGELHDYK
                     NAFADDSRSLLLDSSVGQTYINRSIAAFALRRYELAKHDLDKGQSLGAPASPSFVQML
                     DRALASPVIDSLTQEIKKQPYNPFLYNDRGLKWMEAGRYKNALDDFTKALELAPGEKT
                     LEQNRDSAMYFLQKQN"
     gene            957..2030
                     /gene="corA"
                     /locus_tag="VFU15_06895"
     CDS             957..2030
                     /gene="corA"
                     /locus_tag="VFU15_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013194790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HEU4717540.1"
                     /translation="MQHLSLKSGVPPGTLMYLGRERTEKVKITLIEYNEQEYIEQEFF
                     DIRECLAHVRDGMVKWINVDGVHNVEIVEHIGKLFNIHPLTMEDLVHTSQRPKFEDFE
                     NYLFTIMKMIYEDVNIRQFHDDALVSEQLSILLMSDNTVLSFQEAEAGDVFDIIRSRL
                     RTGKGKIRKSGADYLAYAMIDAVVDSYFSVLEKVGDEIEELEESVIDNAVPETLKQLH
                     HLKRQMVYLRKSVWPMRELINSLERSESDLITEPTTVYLRDVHDHIIRVIDTVETYRD
                     LLSGMMDLYMSSISNRMNEVMKTLTIITTIFVPLTFIAGVYGMNFEFMPELHSKIAYP
                     IIWVIMIGITAALVMYFRRKKWL"
     gene            2044..>2734
                     /locus_tag="VFU15_06900"
     CDS             2044..>2734
                     /locus_tag="VFU15_06900"
                     /inference="COORDINATES: protein motif:HMM:NF017519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX/YebB-like N1pC/P60 family cysteine
                     hydrolase"
                     /protein_id="HEU4717541.1"
                     /translation="MKKNFLFVPLLALAVSCGENSKPADQQKNVDSAITAQENSTKPI
                     APQDIPRSTDTVKMVALPPVMEGDIIFQTWNSDQSDEMEEASGSKYNSVGLVFIRKTD
                     NQYMVVEMRDSLRATPLSEWVSRGKDQHVLLMRLKNSNQILSAKKTNDLRNAVRTYHK
                     MPYDYALSWGDDAMYGSELVWKIYHNALKIDLCPTKQLKDLNTKSGRLKTELGKKYGK
                     NIPLTDPAVTPD"
BASE COUNT          687 a          763 c          733 g          551 t
ORIGIN      
        1 tggtgctgtt cctgaaattc ggtttccgca gcaaacgtct cgcgcttttc ggaatggcct
       61 ggttcatcgt cgtcctggcg ctgccggtat ggtacggtgt gacgaacggg agcggggagc
      121 aatatgaaca ccgcgtatac actgcgtcgc cgggcgcgtt tcttttcctc ctccagctga
      181 agctgcccga agcgtaccgg aaaaccgcgc aacggttcgc gctgctcatg atcgtgtttt
      241 tcgtggtgaa gacatccttc cgcctgccgg tttaccacga tgaattcgtt tacgcaaaag
      301 ccggaacgga agaagctcct tccacatcgt tctttcacga tatgctcggg ttcatgtacc
      361 agcagaagaa ccagtgcacg gatgcattgt cgtgctacaa ccgcgcgatc gcgctcgaca
      421 gcgggaatgc tgaatattac aaccaccgcg gcagcgttta cggggaactg cacgattata
      481 aaaacgcttt cgccgatgac agccgctctc tgctgctcga ttcgtccgtg gggcagacct
      541 acatcaaccg cagcattgct gctttcgcgc tgcgcaggta tgagctggcg aagcacgatc
      601 tcgacaaagg gcaatcgctc ggggcgccgg cttcaccttc gttcgtgcag atgctcgacc
      661 gcgcgctcgc ttcaccggtc attgactcgt tgacgcagga aataaagaag cagccgtaca
      721 atccgttcct ctacaacgac cgcggactga aatggatgga agccggccgg tataaaaacg
      781 cgctcgacga ttttacaaag gcgctggagc tggcacccgg agaaaaaacg ctcgagcaga
      841 accgggattc cgccatgtat ttcctgcaga agcagaactg aaagcagaac cgcttcccgt
      901 tgcgaacgat ttcttatctt tcgtatcccg cacgccccgg gaaactcgac cgttttatgc
      961 agcacctttc cctgaaatca ggcgtaccgc caggcaccct gatgtacctc ggacgtgaac
     1021 gtaccgaaaa ggtgaagatc acgctcatcg aatacaatga gcaggaatac atcgagcagg
     1081 agtttttcga catcagggaa tgtcttgcgc acgtgcggga cggcatggtg aagtggatca
     1141 acgtcgacgg cgtgcataac gtggagatcg tcgagcacat cgggaagctg ttcaacattc
     1201 acccgctgac gatggaggat cttgtgcaca ccagccagcg gccgaaattc gaagacttcg
     1261 agaattacct tttcacgatc atgaaaatga tctacgaaga tgtgaacatc cgccagttcc
     1321 acgacgacgc gctcgtttcc gaacagctgt ccatcctgct catgagcgac aacacggtcc
     1381 tttccttcca ggaggccgag gccggggatg tgttcgacat tatccgctcg cgcctgcgca
     1441 cgggaaaagg aaagatccgg aaatccggcg cggattatct tgcctacgcg atgatcgatg
     1501 cggtggtcga ttcctatttc tccgtgctcg aaaaagtcgg tgacgagatc gaggagctgg
     1561 aagaatccgt gatcgataac gccgttcccg aaacgctgaa gcagctgcat cacctgaaac
     1621 ggcaaatggt ctacctgcgg aaatccgtct ggcccatgcg cgagctcatc aacagcctcg
     1681 agcggagcga atccgatctc atcaccgaac cgacaacggt ttacctccgc gacgtgcatg
     1741 atcacattat ccgcgtcatc gacacggttg aaacgtaccg cgacctgctt tcgggcatga
     1801 tggacctcta catgtcgagc atcagcaacc gcatgaacga agtgatgaaa acgctgacga
     1861 tcatcacgac gatcttcgtt ccgcttacgt tcatcgccgg tgtgtacgga atgaatttcg
     1921 aattcatgcc ggagctgcat tcaaagatcg cgtaccccat catctgggtg atcatgatcg
     1981 ggatcacggc tgcgctcgtg atgtatttcc gcagaaaaaa atggctctga taaaaaacaa
     2041 accatgaaga aaaatttcct tttcgtcccg ctgcttgcac tcgccgtttc ctgcggagag
     2101 aacagcaagc ccgccgacca gcagaagaac gtcgactcgg ccatcacggc ccaggagaac
     2161 agcacgaagc ccattgcacc gcaggatata ccgagaagca ccgacacggt gaagatggtg
     2221 gcgctgccgc ccgtgatgga aggcgatatt attttccaga cgtggaacag tgatcagtcg
     2281 gatgaaatgg aagaggccag cggatcgaaa tacaattcgg tcggacttgt ttttatccgg
     2341 aagacagaca accagtacat ggtggtcgag atgcgcgaca gcctgcgggc cacgccgctg
     2401 tccgaatggg tgagccgtgg aaaagaccag cacgtactgc tcatgcgcct gaaaaattcc
     2461 aaccagatcc tcagtgcgaa aaaaacaaac gacctgcgca acgcggtccg gacgtatcat
     2521 aagatgccat acgattacgc gctgagctgg ggcgacgatg cgatgtacgg cagcgagctc
     2581 gtctggaaga tctaccacaa tgcgctcaag atcgatctct gcccgacaaa acagctgaag
     2641 gacctcaaca cgaaaagcgg gcgactgaaa acggagctcg gtaaaaaata cgggaagaac
     2701 attccgctca ccgatcccgc cgttacaccc gatg
//
LOCUS       DASTSF010000238         3432 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_770108,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000238 DASTSF010000000
VERSION     DASTSF010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3432
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_770108"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1406)
                     /locus_tag="VFU15_06905"
     CDS             complement(<1..1406)
                     /locus_tag="VFU15_06905"
                     /inference="COORDINATES: protein motif:HMM:NF015291.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HEU4717542.1"
                     /translation="MPLLNSIASWLMVKRMHQIELFMKYPHEVQGEWFTRLIEAGRET
                     EFGKKFHFEEINTPEEFSARVPLQNYDSLKTYIDRLRRGEQHLLWPTEVKWFAKSSGT
                     TNDKSKFIPVTDESLRDCHFRGGKDMVSIYCSNYPDHQLFTGKNLALGGSHQSDLFED
                     YESYNGDVSAIIIRNLPLWAEMFRAPSVEIALMSEWTEKLERIAESTLEENVVSLAGV
                     PSWMIVLLRRVLQKSGKKTIREVWPSLEVYFHGGVSFDPYRAQVKEIIGGDLRLMELY
                     NASEGFFGIQDQRDSSDLLLMLDYGIWYEFLPQEEWEKEDSAAAIPLSEVKTGIPYAM
                     VISTTGGLWRYNLGDTIEFTSVQPYRFHITGRTKLFLNAFGEEVMIGNAEKALQIACE
                     KTGSSIVEFSVAPVFMEGKERGAHEWVIEFEKAPENSGYFSELLDNALKSLNSDYEAK
                     RYNSFVLGPPVITPVSSGT"
     gene            complement(1597..3213)
                     /locus_tag="VFU15_06910"
     CDS             complement(1597..3213)
                     /locus_tag="VFU15_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEU4717543.1"
                     /translation="MTTEAIADALKLTAQLMELHGENPFKVKALSNAAFRLDKTNLDL
                     DGKSPEELEAIEGIGKGIAGKIHELQQTGTTKELAALLEKTPAGVVEMLNIKGIGPKK
                     IALLWKELGIESPGELLYACNENRLVELKGFGEKTQAAVKKSLEFSQASKGKFHYADV
                     EHVAEKIVALLKKQLHTEHVSVTGSVRRKDIIIESIEIVAAGDEKKIDLEKADNPMQI
                     PVHVHTCSAGEFFDVLFQTTGNAAHFKLLEHLEKKPKPHAHSEEEIFTELGLQFIPPE
                     LREGHGEITLAKHHKIPALITEKDLAGVLHNHSTYSDGMNTLEEMALHCKENGYEYFG
                     ICDHSQSAFYANGLKADRVAQQHREIDELNRKLYPFRIFKGIESDILNDGSLDYPKDV
                     LASFDFIVASVHSNLKMDKEKATARLLKAIENPYTRILGHPTGRLLLSREGYPVDHKK
                     IIDACAANGVVIELNAHPYRLDIDWKWIPYCMEKGVLISINPDAHEKAGLDMHWGVCA
                     ARKGMLTKEFCLNAKSLEEFEKWVNARREM"
     gene            complement(3228..>3432)
                     /locus_tag="VFU15_06915"
     CDS             complement(3228..>3432)
                     /locus_tag="VFU15_06915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717544.1"
                     /translation="CLTFGAKTCLCSSGSLRQRKRDVKFRAHEMNLNLKITVQMNYFS
                     RNFPCDPFFIFPIVGSAELFAEF"
BASE COUNT          681 a          980 c          883 g          888 t
ORIGIN      
        1 gttccgctgc ttaccggcgt gatcaccggc ggacccagca cgaagctgtt gtagcgcttc
       61 gcttcgtagt cggagttcag cgacttcagc gcattgtcga gcagctcgga aaaatagccg
      121 ctgttttccg gcgctttttc aaattcgatc acccattcgt gagcgccgcg ttcctttcct
      181 tccatgaaaa caggcgccac ggaaaactca acgatggagc tgcccgtttt ttcgcacgcg
      241 atctgcagcg ctttttctgc gttgccgatc atcacttctt cgccgaacgc gttcaggaat
      301 aattttgtcc ggcccgtgat gtggaaccgg tacggctgca cggacgtgaa ctcgatcgtg
      361 tcgccgaggt tgtagcgcca gagtccgccc gtcgtggaga tcaccatcgc atagggaatt
      421 cccgttttca cttcgctgag cgggatcgcg gctgcgctgt cttctttttc ccattcttcc
      481 tgcggaagga attcgtacca gatgccgtaa tcgagcatca gcagcaggtc ggatgaatcg
      541 cgctggtcct gtatcccgaa aaatccttcc gaagcgttgt acagttccat cagccgcagg
      601 tcgccgccga tgatctcttt cacctgcgcg cggtacgggt cgaagctcac gccgccgtgg
      661 aaatacactt ccagcgacgg ccacacttcg cgtatcgttt tctttcccga tttctgtagc
      721 acgcgccgca gcagcacgat catccacgaa ggcacgccgg ccaggctgac gacgttttcc
      781 tctaatgtcg attccgcaat gcgttccagc ttttccgtcc attcgctcat cagcgcgatc
      841 tccacgctcg gcgcacggaa catttccgcc cacagcggaa ggttgcggat gatgatcgcg
      901 gaaacgtcgc cgttgtacga ttcataatct tcgaaaagat ccgactggtg gctgccgccg
      961 agcgccaggt tcttccccgt gaacagctgg tgatcgggat agttgctgca gtaaatggaa
     1021 accatgtcct tgccgccgcg gaaatggcag tcgcgcagcg attcgtcggt cacgggaatg
     1081 aatttgcttt tgtcgttcgt cgtgcccgac gatttcgcga accatttcac ttccgtcggc
     1141 cacagcaggt gctgctcgcc gcgccgcagg cggtctatgt aggtcttcag cgaatcgtag
     1201 ttctgcagcg gaacgcgcgc ggaaaattct tccggcgtat tgatctcttc aaaatggaac
     1261 tttttcccga actccgtttc ccgccccgct tcgatcagcc gcgtaaacca ttctccctgc
     1321 acttcgtgcg ggtatttcat gaacagctct atctggtgca ttcgtttcac catgagccac
     1381 gacgcgatgg aattcaggag aggcatacag gttaaaattc aaacgatttg aaaaaaatca
     1441 ggaattaaaa aaatgaaaga tcgccgattc aaagtaaagc ttattgcagt gatccgggcg
     1501 tgtccgcgga tccccgcggc cgggctgctt ccggctccgt gttcactgcg gtgcttcgca
     1561 cttaaccgtc cgcatccctc acgcaaaaaa aagaactcac atctcccgcc gcgcgttcac
     1621 ccatttctca aactcctcca gcgattttgc gttcaggcag aattctttcg tgagcattcc
     1681 ttttcgcgca gcacacacac cccagtgcat gtcgaggccg gccttttcat gcgcatccgg
     1741 gttgatggaa atgagcacgc ctttttccat acagtacggg atccacttcc agtcgatgtc
     1801 gagacggtac gggtgcgcat tcagctcgat caccacgcca ttggcggcgc acgcatcgat
     1861 tatttttttg tggtcgaccg ggtaaccttc gcgcgaaagt aaaagacgcc cggtcgggtg
     1921 acccaggatg cgcgtgtacg gattttcgat cgctttcagc aaacgcgccg tcgctttttc
     1981 cttgtccatt ttcaggttgg agtgaacgga agcgacgata aaatcgaacg aagcgagcac
     2041 atccttcggg taatcgagcg aaccgtcatt gagtatatcc gactcgatcc ctttgaaaat
     2101 gcggaacggg taaagtttcc ggttcagctc gtcgatctcg cggtgctgct gcgccacgcg
     2161 gtcggctttc agcccgttcg cataaaacgc cgattgcgag tgatcgcaga tgccgaaata
     2221 ttcataaccg ttttctttgc aatgcagcgc catttcttcc agcgtgttca tgccgtcgct
     2281 gtaggtgctg tggttgtgca gcacgccggc caggtctttt tcagtgatga gcgccggaat
     2341 tttatggtgc ttcgccagcg taatttctcc gtggccttcg cgcagctcgg gagggatgaa
     2401 ctgcaggccg agctccgtaa aaatttcttc ttccgaatgc gcatgcggct tcggtttttt
     2461 ttcaagatgc tccagcagtt tgaaatgtgc ggcgtttccc gtcgtctgaa aaagcacgtc
     2521 gaaaaattca ccggcgctgc aggtgtgtac atgcacgggg atctgcatcg ggttgtccgc
     2581 cttctccagg tcgatctttt tttcatcgcc ggcggcgacg atctctatcg attcgatgat
     2641 gatgtctttc cggcgcacgc ttcccgtcac ggaaacgtgt tcggtgtgca gctgcttttt
     2701 caggagcgcg acgatctttt ccgccacgtg ctccacatcg gcataatgga attttccttt
     2761 gctcgcctgc gaaaattcca gcgatttctt cacagccgcc tgtgtttttt caccgaaacc
     2821 tttcagctcg acaaggcggt tctcgttgca ggcatacagc aattcacccg gcgattcgat
     2881 gcccagctct ttccatagca gcgctatctt tttcggaccg atccctttta tgttcagcat
     2941 ttccaccacg ccggccggtg ttttttccag cagggcggcg agctccttcg tggtgccggt
     3001 ctgctgcagc tcgtggatct ttcccgcaat ccctttgccg atgccttcga tcgcttccag
     3061 ctcttccggc gatttcccgt cgagatcgag gttggttttg tccagcctga atgcggcgtt
     3121 cgaaagcgct ttcactttaa acgggttctc gccgtgcagc tccatcagct gtgcggtgag
     3181 cttcagcgcg tctgcaattg cttctgttgt catcgcggtt atttgcatta aaattccgca
     3241 aataattccg cactcccaac aatcgggaag atgaaaaatg gatcacaggg gaaattccgg
     3301 gagaagtaat tcatctgaac agtgattttc aagttgaggt tcatttcatg cgcccggaat
     3361 tttacatccc gttttctttg ccgtaaagac ccggaggaac aaaggcaggt tttcgcgccg
     3421 aaagtgaggc at
//
LOCUS       DASTSF010000239         6089 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_90789,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000239 DASTSF010000000
VERSION     DASTSF010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 6089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6089
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90789"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..559
                     /locus_tag="VFU15_06920"
     CDS             <1..559
                     /locus_tag="VFU15_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015809821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEU4717545.1"
                     /translation="ALILGDNIFYGAGLSGLLQQNNDPEGGVVFAYHVSDPERYGVVE
                     FDKNGDVLSIEEKPKQPKSSFAVPGLYFYDNSVVEIAKKLEPSARGEYEITDVNKKYL
                     EAGKLKVGVLQRGTAWLDTGTFTSLLQAGQFVQVIEERQGYSIGCIEEVAYRMKFIDS
                     AQLKALAAPLMKSGYGQYLLNLTGQ"
     gene            571..1521
                     /locus_tag="VFU15_06925"
     CDS             571..1521
                     /locus_tag="VFU15_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020036194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEU4717546.1"
                     /translation="MSTPHKKNILVTGGAGFIGSAITEKLAADPSHNVIVIDNLLTGD
                     EKRVTSSGRPNISFIRGNVNSHNDISAIFLSNRIDYVFHYAAVVGVQRTLQNPVMVLK
                     DILGMRHITSLAKTAGVKRIFFSSSSEVYGEPVEFPQNEETTPLNSRLPYAIVKNAGE
                     AFLKAFRQEFGLHYTIFRFFNTYGPQQSSDFVMSKFLRAALKNEPLYIHGDGSQSRTF
                     CYIDDNTDACLSAFYGNKCVDEVVNIGNDNEISILDLARTIIRVTGSRSEIIHGPALK
                     EGDMTRRRPDVTKMKQLLGRDMLPLEEGIRRILNDPKFIS"
     gene            1522..2499
                     /locus_tag="VFU15_06930"
     CDS             1522..2499
                     /locus_tag="VFU15_06930"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEU4717547.1"
                     /translation="MDTTAFNRLISESLEKFSAQLPVQPASLYDPIRYFIANGGKRMR
                     PLLVLYGAGIFSRDVSRAVPAATGIELFHNFTLLHDDIMDNAVMRRGKETVHVKWNTN
                     VAILSGDALFAEAFRQVAMSPPDVLPRVLGIFSRTATEVCEGQQLDMDFENAPQVSIA
                     GYMQMIRLKTAVLLRASLSIGALCGGAAENDAQLLGAFGEHTGLAFQLQDDILDVFGD
                     EDKFGKVTGGDIVSNKKTFLLLKAMELANRYQREELQHWMLVPVSEAAAKVEAVKNIY
                     NTLGVRELAEKEMQNQYAHATAALEKVNAPGENKMMLKAFSDALMVRQH"
     gene            2502..2879
                     /locus_tag="VFU15_06935"
     CDS             2502..2879
                     /locus_tag="VFU15_06935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717548.1"
                     /translation="MKPSRLLALISFFLSATTETAARTGHSAVIHVDLLALLFFILAI
                     GLWRKNARALKWFRRTLWFLFVILAAILFFTLSGFDFRFRVFHSANGIIAVLVLLLIA
                     CGALIYATETGKVLADFGLAGND"
     gene            complement(2883..3251)
                     /locus_tag="VFU15_06940"
     CDS             complement(2883..3251)
                     /locus_tag="VFU15_06940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717549.1"
                     /translation="MKALSFLFIFFLPCTLRLSAQSRPDSLSRDSVIARMDYCTCDSL
                     LPVKQSWSVYDQTSGNATPITGYYRFDHVQGKSTAKCGKFKDGMLSYGFLFVYDENGM
                     LIETRKYYDSRYVGSCHVQE"
     gene            complement(3274..3987)
                     /locus_tag="VFU15_06945"
     CDS             complement(3274..3987)
                     /locus_tag="VFU15_06945"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010572025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEU4717550.1"
                     /translation="MKNSGEYVTLNVKDGTEMRAYVARPSSGDGPFPGLLIFQEAFGV
                     NSHIRDLAHRFAAEGYVCIAPELFHRTAPGFEGDYNNFPAIAPHMQALTEEGLLADAV
                     AAYEWLIAQSDVRTGAIAGTGYCMGGRIAFLANTALPLKAAVSYYGGRIVPDLVKKTA
                     QLHAPMLFFWGGLDKHIPAEHVQEVVAALKKEGRPHVSVEISYADHGFFNDQRTAYHP
                     QAAKEAWALTLAFLKNKLA"
     gene            4104..5588
                     /locus_tag="VFU15_06950"
     CDS             4104..5588
                     /locus_tag="VFU15_06950"
                     /inference="COORDINATES: protein motif:HMM:NF017889.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007160 - cell-matrix adhesion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nidogen-like domain-containing protein"
                     /protein_id="HEU4717551.1"
                     /translation="MKKTLLLCTALASCGLISAQQAVSVDQYEQMKSAGQLNPQTNYI
                     FVTPDRQLHDNSNARHSNPVQAQSSTCNCLIPLDTTFNVVPFTGGLPPDYRNDDGFTN
                     VIQLPFTFNFYGTNYDSIFINNNGNISFGTPYSTFTANPFPDPNYSMIAPFWADVDTR
                     DSVSGLVYYKITPTHMIVKWENVGYYNMHNDKLNTFQLIMTDGQDTILPPGSNVAFCY
                     GDMQWTTGDASSGLNGFGGTPSTVGCNKGNGVDYFQVTQNDSSGNTFDGPYGSADGVD
                     WLDNQEIYFNTSLVGNIPPLVMNSTICDTIDVYTGDTLRSMNIDSIAFDMMFMTPEPN
                     QQLVSTSITSSAPSALTYTQASNSATYQSYSCVFHARNLPPGLYTVTGTATDNGTPAG
                     TNTGTVYIRTYYDISLDGIQENSAAGAISIYPNPADDQLTVETPGMNNATLVITDISG
                     RVVYTGAATGNKTSIDVSSFGAGVYFVSVCSADGEKQALKFVRK"
     gene            complement(5688..>6089)
                     /locus_tag="VFU15_06955"
     CDS             complement(5688..>6089)
                     /locus_tag="VFU15_06955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717552.1"
                     /translation="PCMAAMEPGDTAYFSWHFAEIFAPGRFAIVAREYGGMCGSGGER
                     GDYVSFFIIAADTSGFYTVYDDVQETSSYSMASPALTMPVFRRYRIVPGAAAPFPRRL
                     KITETFFELGKGQYDFPKETGKKVSYADLER"
BASE COUNT         1398 a         1738 c         1597 g         1356 t
ORIGIN      
        1 ggcgcttatc ctcggcgata atatttttta cggcgcagga ctcagcggcc tgctgcagca
       61 gaacaacgac ccggaaggcg gcgtggtgtt tgcataccac gtttccgatc ccgaacgcta
      121 cggcgtcgtt gagttcgaca agaacggcga tgtcctttcc attgaagaaa aaccgaagca
      181 gcctaaatcg agcttcgcgg tgccgggact ttatttttac gacaacagcg tggtggagat
      241 cgcgaagaag ctcgagccga gcgcgcgcgg tgaatacgaa ataaccgacg tgaataaaaa
      301 atacctcgag gccggaaagc tgaaagtggg cgttctccag cggggaaccg cctggctcga
      361 cacgggaaca ttcacttcgc tgctgcaggc cgggcagttc gtgcaggtga tcgaggagcg
      421 gcagggctac agcatcgggt gcatcgagga agtggcgtac cggatgaaat tcatcgattc
      481 cgcacagctc aaagcgcttg ccgcaccgct gatgaaaagc gggtacggcc agtaccttct
      541 caatcttaca ggacagtaat tttccacgcg atgagtaccc cgcacaaaaa aaacatcctg
      601 gttaccggtg gcgccggttt catcgggagc gccataacgg aaaagcttgc ggcggatcct
      661 tcgcacaacg tcatcgtgat cgacaacctg cttaccggtg atgaaaaacg ggtgacatcg
      721 tccgggcgtc cgaacatcag cttcatccgc gggaacgtca acagccacaa cgatatttca
      781 gcgatcttcc tcagcaaccg catcgattac gtttttcatt acgcggccgt cgtcggggtg
      841 cagcgcacgc tgcagaaccc tgtcatggtg cttaaagaca ttctcggcat gcgtcacatc
      901 acgagccttg ccaagacggc cggcgtcaaa cggatctttt tttcctcttc ttcggaagtg
      961 tacggcgaac cggtggaatt tccgcagaac gaagaaacca cgccgctgaa ttcgcgtctt
     1021 ccctatgcga tcgtgaaaaa tgccggtgaa gcattcctca aagcgttccg ccaggaattc
     1081 gggctgcatt acaccatctt ccgctttttc aatacgtatg gtccgcagca gagctctgat
     1141 ttcgtcatgt cgaaattcct ccgcgccgcg ctgaagaacg agccgctgta tattcacggc
     1201 gacggcagcc agtcgcggac attctgctac atcgacgaca acaccgacgc ctgcctcagc
     1261 gctttttacg ggaacaaatg cgtcgatgaa gtggtgaaca tcgggaatga caacgagatc
     1321 tccattctcg atctcgcacg cacgatcatc cgcgttaccg gttcacgatc tgagatcatt
     1381 cacggtcccg cactgaaaga aggcgacatg acccggcgca gacctgacgt caccaagatg
     1441 aagcagctgc tcggccgcga catgctcccg ctcgaagaag gtatacgcag gatcctcaac
     1501 gatccgaaat tcatttcctg agtggacacg accgcgttca accggctcat cagcgaatca
     1561 ctggaaaaat tttcagcgca gctgcctgtg caacccgctt cgctgtacga tcccatccgt
     1621 tacttcatcg cgaacggagg caagcgcatg cggccgctgc tcgtgctgta cggcgccggc
     1681 attttttccc gtgacgtttc acgcgctgtt cctgccgcca cgggcataga gctgttccac
     1741 aatttcacgc tgctgcacga cgacatcatg gacaatgccg taatgcggcg cgggaaggaa
     1801 accgtgcatg tgaaatggaa tacgaacgtc gctattctca gcggtgatgc gcttttcgcg
     1861 gaagcgttcc gccaggtggc aatgagtccg cccgacgtgc tcccgcgcgt gctcggtatt
     1921 ttttcccgca ccgcaacgga agtgtgcgaa ggccagcagc tggacatgga ttttgaaaat
     1981 gcgccgcagg tttccatcgc gggatacatg cagatgatca ggctgaaaac agccgtgctg
     2041 ctccgggcat ccctgtcgat cggtgcgctc tgcggcggtg cggcggaaaa cgatgcgcag
     2101 ctgctcggcg ctttcgggga acataccggc cttgctttcc agctgcagga cgatatcctc
     2161 gatgtgttcg gcgatgaaga taaattcggg aaggttaccg gcggcgatat cgtgtcgaac
     2221 aaaaaaactt tcctgctcct gaaagcgatg gaactcgcca accgttacca gcgggaagaa
     2281 ctccagcact ggatgctcgt tcccgtttcc gaagcggcgg cgaaggtgga agccgtgaag
     2341 aatatttaca acacgctcgg ggtgcgcgag ctcgcggaaa aagaaatgca gaaccagtat
     2401 gcacacgcca ccgcggcatt ggaaaaagtg aacgcgcccg gggaaaacaa gatgatgctg
     2461 aaagcttttt cggatgcgct gatggtaaga cagcactgac gatgaaaccc tcccgcctcc
     2521 tcgcgctcat ttcttttttt ctttccgcga cgaccgaaac tgccgcaaga accgggcata
     2581 gtgcggttat tcacgtcgac ctgcttgccc ttcttttttt catactggcg atcggcctgt
     2641 ggagaaagaa cgcgcgtgcc ttgaaatggt tccggcgaac gctgtggttt ttgttcgtga
     2701 tccttgcagc gatcctcttc ttcacccttt cgggttttga cttccgcttc cgcgttttcc
     2761 attctgcaaa cgggatcatc gcggtgctcg ttttattgct gatcgcatgc ggggcattga
     2821 tctatgccac ggaaaccggt aaagtgcttg ctgattttgg cctggccgga aacgactgac
     2881 cgtcactcct gcacatggca gcttcccacg tacctgctgt cgtaatattt ccgcgtttcg
     2941 atcagcatcc cgttttcgtc atagacaaaa aggaatccgt aactgagcat gccgtctttg
     3001 aacttcccgc atttcgccgt ggattttccc tgcacgtgat cgaaacggta atagcccgtg
     3061 ataggcgttg cgttccccga cgtctgatcg tacaccgacc agctttgttt caccgggaga
     3121 agactgtcgc acgtgcagta atccattcgc gcgatcacgg aatcgcggct gagcgaatcc
     3181 ggcctgcttt gcgcagaaag acggagcgtg caggggagaa aaaaaatgaa gaggaatgaa
     3241 agtgctttca tgtgtacgcg gttacagcga aaatcaggcc agtttgttct tcaaaaatgc
     3301 aagcgtgagc gcccatgctt ccttcgccgc ctgcgggtga taagccgtgc gctgatcgtt
     3361 gaagaagcca tgatctgcgt aggaaatttc cacgctcacg tgcggccggc cttccttctt
     3421 cagcgctgca acgacttcct gcacgtgttc cgcaggaata tgcttatcga gtccgcccca
     3481 gaaaaacagc atgggtgcgt ggagctgcgc cgtttttttc acaaggtcag gcacaatcct
     3541 tccgccgtaa tacgatacgg ccgccttcag cggcaacgcc gtgttggcaa ggaacgctat
     3601 gcgtccgccc atgcaataac ccgtaccggc gatcgcaccg gtccgcacat cactttgcgc
     3661 gatcagccat tcgtaagcgg ctacggcatc ggcaagcaat ccttcttccg tcagcgcctg
     3721 catgtgcggg gcgatagcgg gaaaattatt gtaatcgcct tcgaagccgg gcgctgtgcg
     3781 gtgaaaaagt tcgggcgcaa tgcacacgta accttctgct gcaaaacgat gcgcgagatc
     3841 gcggatatgg ctgttgaccc cgaacgcttc ctggaaaatg aggaggccgg gaaaaggtcc
     3901 gtcgccggaa gaagggcgcg cgacgtatgc cctcatttcc gttccgtcct tcacgttcag
     3961 cgtaacgtat tcgccggaat ttttcatttg aatttcctgt taacggggcc ttgatgtaaa
     4021 aatagtggtt tcccgcgtcc cggctgttgc atttgagaaa accatggctt atctttaatt
     4081 aagcaaacac aaacacttct ttcatgaaaa aaaccctact cctttgcacg gcgcttgcat
     4141 cctgcggtct catttccgcg caacaggctg tttccgtcga ccagtacgaa cagatgaaaa
     4201 gcgcggggca gctgaacccg cagacgaatt acattttcgt cacacctgac cgccagctgc
     4261 atgacaacag caacgcacgc cacagcaatc ctgtgcaggc gcaaagctcc acctgtaact
     4321 gcctgatccc gctcgacacg acattcaacg tggtgccttt taccggtggt ctgccgccgg
     4381 attaccgcaa cgacgacggg ttcaccaacg tgatccagct cccgttcaca ttcaattttt
     4441 acggaacgaa ctatgactcg atcttcatca ataacaacgg gaacatttcc ttcggcacac
     4501 cgtattccac gttcacggca aacccgttcc ccgatccgaa ctacagtatg atcgcgcctt
     4561 tctgggcgga cgtggacacg cgcgattcgg taagcggtct tgtctattac aagatcacgc
     4621 ctacgcacat gatcgtgaag tgggaaaatg tcggttatta caacatgcac aacgacaagc
     4681 tcaacacgtt ccagctgatc atgaccgacg ggcaggacac gatccttccc ccgggatcca
     4741 acgttgcttt ctgctacggc gatatgcagt ggactacggg tgatgcttcg tcaggcctca
     4801 acggtttcgg gggaacgccc tccaccgtcg gatgcaacaa agggaacggc gtggattatt
     4861 tccaggtgac acagaacgat tcttccggaa atacatttga cggtccgtac ggttccgccg
     4921 acggcgtcga ctggctcgac aaccaggaaa tttatttcaa caccagcctt gtcgggaaca
     4981 ttcccccgct tgtgatgaac agcacgatct gcgatacgat cgatgtgtac accggcgaca
     5041 cgctgcgcag catgaacatc gattccattg cgttcgacat gatgttcatg acgccggaac
     5101 cgaaccagca gctggtttcg acttccatca cgtcttcagc tccgtcagcg ctcacgtata
     5161 cgcaagcttc gaattcggca acttaccagt cttattcgtg cgtcttccat gcccgcaatc
     5221 ttcctcccgg actttacacc gtaacgggaa ctgccaccga caacggtacg ccggccggaa
     5281 caaataccgg aaccgtttac atccgtacgt attacgacat ttcgctcgac ggcatccagg
     5341 aaaattccgc tgccggtgcg atcagcattt acccgaatcc tgcggatgac cagctcacgg
     5401 tggaaacacc gggcatgaac aatgcaacgc tcgtcatcac ggatatttcg ggtcgcgtgg
     5461 tttatacggg tgcagcaacc ggtaacaaaa ccagcatcga tgtttcttcc ttcggtgccg
     5521 gcgtttattt tgtttctgtc tgttccgcgg acggagaaaa acaggcgctg aaatttgtgc
     5581 ggaaataaaa tcgcactgat cccctaacga cgatgccggc ggaaatgccg gcattgttgt
     5641 tttatccttt caggccaggt gaaatttatt ttttcgccgg gatcatttca tctctccagg
     5701 tcggcatagc tcaccttttt cccggtttct ttcggaaaat cgtactgtcc ttttcccagc
     5761 tcgaaaaatg tttccgtgat cttcagccgc cgcgggaatg gcgcagctgc gccgggcacg
     5821 atgcggtagc ggcggaaaac cggcattgtg agcgccggtg aagccatgga atagctgctc
     5881 gtttcctgca cgtcatcgta taccgtgtaa aacccggacg tgtcggcagc aatgatgaaa
     5941 aaggaaacat aatcaccgcg ctcacccccg cttccgcaca ttccgccata ttcgcgcgca
     6001 acaatggcga aacggcccgg tgcgaagatc tctgcaaagt gccacgagaa atacgccgtg
     6061 tcgccgggct ccatggctgc catgcacgg
//
LOCUS       DASTSF010000240         5199 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_15785,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000240 DASTSF010000000
VERSION     DASTSF010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5199
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15785"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1149
                     /locus_tag="VFU15_06960"
     CDS             <1..1149
                     /locus_tag="VFU15_06960"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEU4717553.1"
                     /translation="SIPVNDSIVQEVVVVDTTQPSVRLSYTRNQTDQVGAIISWGGGS
                     GGVGMYFIPPTYPARILNTNFVIPFAPSSGVAFYSKIYADDGPAGGPGTLLDSEAVQA
                     TSINLNGLTIVPVTNNVVLTSGGYYVLWDMANPSVGIAEDVTPPFSRRSYEVIQGYWS
                     TYRDYQVSDFFIGSDYFSGAPEDVGVSSIFNPQPNAAITGSGTNVSCWIKNYGGSPDN
                     YFITVNYKLGNYPNIVSQPYTGPAILPGDSVLFTFSTPLVAPGFGNDILYVWTQKTLD
                     VDHSNDTDRVAVNLVGMNELSAIPGLRVSPVPANDHVTFTFASGLPGDGVIIITDLSG
                     KIVAQKKTGVLAPGEKTEIDISSLANGAYFYTVKTAEQTANGKIVKTD"
     gene            1190..3505
                     /locus_tag="VFU15_06965"
     CDS             1190..3505
                     /locus_tag="VFU15_06965"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEU4717554.1"
                     /translation="MKIGLKLTLGFFAVAFASMAVIGVISFIKGKNSLEKESFNRLKA
                     VREMKANQIEDYYRQITDQMQTMAEDPTIIEGMKEFKRGFYSVDKDLGVNDAKMSAIN
                     TQLDSYFDKQYLPRLNRNLDTKAKREDESSTDIHTRILQYLYIASNSYEVGSKYKLDD
                     ARDQSSWTQTHKKFHPILRNYLQKFGYYDVFLVDTTGNIVYTCFKEVDFGTSLETGPF
                     SNTNIAEAYRATKKATTRDFVTLVDFKPYHPSYNASASFIAAPIFDGNEKIGVLIFQM
                     PIDRINDIMTSKREWSKVGLGRTGETYIVGDDFTLRNQSRFLIEDSTNYFRMIRDIGL
                     PELTVNRIRNFNSSIGLQRVRTRGTEAALSGQTGEKIFPDYRGVPVLSSFRPLKIKGM
                     HWVIMSELDEAEAFSAVYLLRSQIFIAFGILIVLLGILSYIFSKRLTRSLTILTEDAR
                     ELARGNFDKEINIKRKDEIGVLAASFRKMQISIKNLVEELREINQNLEKKVEDRTQEI
                     NQQKQLVEHQNKEILDSINYALRLQQAILPTREVIAETLGDSFVLFKPKDIVSGDFYW
                     VRKSGPNVMAAVVDCTGHGVPGALVSIVGASSLGRCVGEFSLKKPSDILEKLNELVIE
                     TFESTDHEIKDGMDLSMVSLEQNHQGVIVHYSGANNPLWIFRAKTGEMEEIKADKQPI
                     GKYDFRKPFTNHEVKMEKGDIIYLFSDGYADQFGGPNGKKFKYSMLKKLLLDIHTKPM
                     EEQRSILDKAFEDWKGELAQIDDVCVMGIRV"
     gene            complement(3517..4920)
                     /locus_tag="VFU15_06970"
     CDS             complement(3517..4920)
                     /locus_tag="VFU15_06970"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEU4717555.1"
                     /translation="MFRNVFILLLLVWSPAGRIAAQCTAAFSFTLNSDSASFVNLSVA
                     QHAHYYWNFGDGSTSYETDPSHRFPESGSYIVTLYLRDTLSGCHAAKDSLLTVTKAST
                     DPCEPGLAYYYFTSAYYDMLSVNDNSSNCGNYHELVDAGPGMNFGAGNSMYVDSSWMP
                     ANFIGRIKYMTDDSINGFVTRRSYFATMPYHYSSAVNYDSCSANFEFSVSYQPSGALL
                     TFHAMDPHQSAYDFMILGMGNPIHLNYDNASFLMPYVYQSDYFPWIIGSYSADSSGCR
                     DTSWQQVLVRNINYVQPPTCAISSQPADQVVIEGNDAYFIAGVDTMTDRQWQMDDGTG
                     FVNLTDAGPFSGVHSDTLVVHNAGMSMNHYRFRCTVLSGLCDCHTTSSAALLTVENGD
                     ISVFPVPAHNSLNIIFTDYRYPSDVVIYNAVGQEVMETEVTGQSATLDISALRSGLYT
                     VSVTTNGTRYARKFAKY"
BASE COUNT         1350 a         1398 c         1374 g         1077 t
ORIGIN      
        1 agtattccgg tgaatgattc catcgtgcag gaagtggtcg tggtggatac gacacaaccg
       61 tcggtgcggc tttcctacac gcgcaaccag accgaccagg taggcgccat catttcctgg
      121 ggcggaggat ccggcggtgt cggcatgtat tttattccgc caacgtaccc tgcgcgaatc
      181 ctgaacacca attttgtgat cccgttcgct ccgtcatccg gcgttgcgtt ctattcgaaa
      241 atatacgccg acgacggtcc ggccggcggc ccgggcacat tgctcgacag cgaagctgtg
      301 caggccacat cgatcaacct caacgggctg accattgttc cggtgacgaa caacgtcgtg
      361 cttacgagcg gaggatatta tgtcctgtgg gacatggcga acccgagcgt ggggattgcg
      421 gaagacgtta cgccaccgtt ctcacgccgt tcgtatgaag tgatccaggg ttactggtcg
      481 acgtaccgcg attaccaggt ttccgatttc ttcatcggct cggattattt ttccggcgca
      541 ccggaagatg tcggcgtatc gagcattttc aacccgcagc cgaacgcggc gatcaccggg
      601 agcggaacga acgtcagctg ctggataaaa aactacgggg gatcgcccga taattatttc
      661 atcaccgtca actacaagct cgggaattac ccgaacattg tttcccagcc ttacaccggt
      721 ccggcgatac tcccgggtga ttccgttctc tttacgttct ccacgccgct tgtcgcaccg
      781 ggattcggga acgacatcct gtacgtatgg acgcaaaaaa cacttgacgt cgatcacagc
      841 aacgacaccg acagggtagc ggtgaacctc gtcggcatga atgagctgag cgccattccc
      901 gggctccgtg tttcacccgt tcctgcgaat gatcacgtga cgttcacttt tgcgtccggg
      961 cttcccggcg acggagtgat catcatcacg gatctttcgg gcaaaattgt cgcgcagaaa
     1021 aaaacaggag tgcttgcgcc gggagaaaaa acggaaatcg acatctcttc gctggcgaac
     1081 ggcgcttatt tctatactgt aaaaacggcc gaacagaccg cgaacggaaa gatcgtaaag
     1141 accgactgac ccttcgtccc atcccaccag catccgtgca ccgctcatta tgaaaatagg
     1201 actcaaacta accctgggat ttttcgcggt tgcatttgcg tccatggccg tcatcggtgt
     1261 gatctctttc atcaagggaa aaaattcgct ggaaaaagaa tcgttcaacc gtctcaaggc
     1321 ggtgcgcgaa atgaaagcga accagatcga ggattattac cgccagatca ccgaccagat
     1381 gcagacgatg gcggaagacc cgacgatcat cgagggcatg aaggaattca agcgcggatt
     1441 ttattctgtg gataaagacc tcggcgtgaa cgacgcgaaa atgtcggcga tcaatacgca
     1501 gctggattcc tatttcgaca agcaatatct tccccgcctc aaccggaacc tcgatacgaa
     1561 agcgaaacgc gaggatgagt cgtccactga tatccacacg cgcatcctgc agtacctcta
     1621 cattgcatcg aacagctatg aagtgggaag caaatacaag ctcgacgatg cccgcgatca
     1681 aagttcgtgg acgcaaacgc acaaaaaatt ccatcccatt ctccgcaatt acctgcagaa
     1741 gttcggttac tacgatgtgt tcctcgtcga cacaacggga aatatcgtgt acacgtgctt
     1801 caaggaagtc gatttcggaa cgtcgctgga aaccggtccg ttcagcaaca cgaacatcgc
     1861 tgaagcttac cgcgcaacga aaaaagcgac cacgcgggat ttcgtcacgc tcgtcgattt
     1921 caagccgtac catccttcct acaacgcctc cgcatcattc atcgctgcgc cgatcttcga
     1981 cggaaatgaa aagatcggcg tgctcatttt ccagatgccg atcgaccgca tcaacgacat
     2041 catgacgagc aagcgtgaat ggtcgaaggt cgggctgggc cgcaccggtg aaacgtacat
     2101 cgtgggcgat gattttaccc tgcgcaacca gtcgcgtttt ctcatcgagg acagcacgaa
     2161 ctatttccgc atgatccgcg acatcgggtt gccggagctg acggtgaacc gcatccgcaa
     2221 cttcaacagc tcgatcggcc tgcagcgtgt caggacgcgg ggaaccgagg ccgcgctttc
     2281 cggccagacc ggcgaaaaaa tatttcccga ttaccgcggc gtgcctgtgc tttcgtcttt
     2341 ccgcccgctg aaaataaaag gcatgcactg ggtgatcatg tcggagctcg acgaagcgga
     2401 agcattcagc gcggtgtacc tcctgcgcag ccagatcttc attgcattcg gcatcctgat
     2461 cgtgctgctc ggcatcctgt cttacatttt ttccaaacgt ctcacgcgct ccctgacgat
     2521 cctcacggaa gacgcgcgcg agctggcacg cggaaatttc gacaaggaaa taaacatcaa
     2581 gcgcaaggac gagatcggcg tgctggccgc cagcttccgc aagatgcaga tctcgatcaa
     2641 aaatctcgtg gaagagctgc gtgagatcaa ccagaacctg gaaaagaaag tggaagaccg
     2701 cacgcaggag atcaaccagc agaagcagct cgtcgagcac cagaacaagg agatcctcga
     2761 ttcgatcaat tacgcgctgc gcctgcagca ggccatcctc ccgacacggg aagtgatcgc
     2821 ggaaacgctg ggcgacagct tcgtgctgtt caagccgaaa gacattgtgt cgggcgattt
     2881 ttactgggtg cgcaaatcag gtccgaacgt aatggccgca gtggtggatt gcacgggaca
     2941 cggcgtgccc ggtgcgctcg tgagcatcgt gggtgcaagc agcctcgggc gctgcgtcgg
     3001 tgaattcagc ctgaaaaaac cttcggacat cctcgagaag ctgaacgagc tcgtgatcga
     3061 aacgttcgag tcgacggacc acgagatcaa ggacggtatg gacctttcga tggtgtcgct
     3121 ggagcagaac caccagggcg tgatcgtgca ctattccggc gcgaacaacc cgctctggat
     3181 cttccgtgcg aaaaccggcg agatggagga aataaaagcc gacaagcagc cgatcggcaa
     3241 atatgatttc cggaagccgt tcaccaatca cgaagtgaag atggaaaaag gagatatcat
     3301 ttatcttttt tcagacggtt atgccgacca gttcggcgga cccaacggaa aaaaattcaa
     3361 gtacagcatg ctgaaaaaac tgctgctgga cattcacaca aaacccatgg aggagcagcg
     3421 cagcatactc gacaaagcgt tcgaagactg gaaaggcgag ctggcgcaga tcgacgatgt
     3481 gtgcgtgatg gggatacggg tgtaaaaata tccgcttcag tacttcgcga acttccgcgc
     3541 atagcgcgtt ccattggtgg tcacgctgac cgtgtaaaga ccggacctca gcgcagaaat
     3601 atccagcgtc gcggattgtc ccgttacttc cgtttccatc acttcctgac caactgcgtt
     3661 atagataacg acatccgaag gatagcggta atccgtgaaa atgatgttga gcgaattatg
     3721 cgcaggcacc gggaaaaccg aaatatctcc gttctccacc gtcagtaacg cagcgctgct
     3781 cgtagtatgg cagtcgcaca gccccgacaa aacagtacaa cggaaacggt agtgattcat
     3841 tgacataccg gcgttatgaa caacgagcgt atccgaatga acaccgctga acggacccgc
     3901 atccgtaaga ttcacgaaac ccgtaccgtc atccatctgc cactggcggt cagtcatcgt
     3961 atccacgcca gcgataaaat aagcgtcgtt accttctatc accacctggt ctgcaggctg
     4021 agaagaaatc gcgcacgtgg gcggctgcac gtaatttata ttcctcacca gcacctgctg
     4081 ccaggaagta tcgcggcacc cgctgctgtc ggcagaataa gagccgatga tccaggggaa
     4141 gtaatcggac tggtacacat agggcataag aaaagatgcg ttatcataat tcaggtgaat
     4201 aggatttccc ataccgagga tcatgaagtc ataggcggac tggtgaggat ccatggcatg
     4261 gaaggtgagc aaagcgcccg aaggctggta gctcaccgaa aattcgaagt tggcgctgca
     4321 ggaatcgtaa ttgacagcgc tggaataatg atacggcatc gtggcgaaat acgaacgcct
     4381 ggttacaaaa ccgttgatcg aatcatcggt catgtatttt atccgcccga taaaattcgc
     4441 cggcatccag gacgaatcga catacatcga attgcccgca ccgaaattca ttccgggacc
     4501 ggcatccacg agctcatggt aattgccgca gtttgaagaa ttatcattca ccgacagcat
     4561 gtcgtagtaa gcgctggtga aataataata cgcaaggccg ggctcgcagg gatccgtgct
     4621 tgctttcgtc accgtaagca gggaatcttt ggcagcatgg caacccgaaa gcgtatcgcg
     4681 caggtaaagc gttacaatat aagagcctga ttccgggaac cggtgtgaag gatccgtttc
     4741 atacgaagtg gatccgtcgc cgaaattcca gtaataatgc gcatgctgcg caaccgacag
     4801 gttgacgaag gaggccgaat ccgaattcag cgtgaacgaa aaagccgctg tacactgcgc
     4861 cgcaatacgt ccggccggcg accataccag gagcaataag atgaaaacgt ttctgaacat
     4921 gactgtcgag gttgaaccgg ggatggcaga ttaaatttaa gccaaattca cctggtggtt
     4981 gcggcaaaac gcataaatcg tcgccctgaa aggaaagtcg ggaaaacagg acgtaacttt
     5041 ccgcggccaa ccggttatca gatcatgaaa aattttcgcc ggatcatcct gttttgtttc
     5101 attgttttca ttcctttcgg tgattgcttc gcgggggact cgtcatccgc cataaacgac
     5161 acggctacct gcatcgcgaa tatcactttc ctgaaaaac
//
LOCUS       DASTSF010000241         5506 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1779629,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000241 DASTSF010000000
VERSION     DASTSF010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5506
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1779629"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..318)
                     /locus_tag="VFU15_06975"
     CDS             complement(<1..318)
                     /locus_tag="VFU15_06975"
                     /inference="COORDINATES: protein motif:HMM:NF021536.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2267 domain-containing protein"
                     /protein_id="HEU4717556.1"
                     /translation="MSLNFEKMNNDGNSFLNDLSDVLGFPGEKQKAGRILKAVLHTMR
                     DHIPVETSLHFLSQLPAFLKAAYVEEWKSGAHRRVHHVEEFLFQVVGNDNVTGTGELD
                     TVED"
     gene            complement(410..1621)
                     /locus_tag="VFU15_06980"
     CDS             complement(410..1621)
                     /locus_tag="VFU15_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017730898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HEU4717557.1"
                     /translation="MSEDKIHILMIDDDEEDFIITKDILLEKSGGRFALEWVSSYEDG
                     LRAFGEKKHDVYLVDYRLGVNNGIDLIREGVAMGVEAPFILMTGQSDPVIDTMALEAG
                     AADFLVKGSISSSQLERAIRYSIEQNKNVMKITKLNAELEERVKERTQTLENALRELK
                     RSEEELAVALEKEKELNDLKSRFVAMASHEFRTPLATILSSLTLVKRYAELGDPEKME
                     KHTKRIGSSVSNMTELLNDVLSLSKLEEGKIVPAPESFSLMQFAEEIIREMRGIAKPS
                     QEILYSHDGDAEDVSTDKKIVRNILHNLVSNAIKFSDDGKKIEVRTSCANDTAMIEVK
                     DNGIGISEEDQKHLFQRFFRGQNVSNIQGTGLGLSILAKYVEVLHGKINMQSELGKGT
                     VFSISFPVSIS"
     gene            complement(1618..2076)
                     /locus_tag="VFU15_06985"
     CDS             complement(1618..2076)
                     /locus_tag="VFU15_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU4717558.1"
                     /translation="MKNPLYPIEILIADDDEEDRMLITDALKESRLKNKIQTVENGEE
                     LMEYLNNKGKFSDTQKYPKPGLILLDLNMPRKDGREALREIKEKHELRSIPIIVLTTS
                     KAEEDILRSYDLGVNSFITKPVTFTAMVDVMKTLNKYWFEIVELPSGGEK"
     gene            complement(2073..3224)
                     /locus_tag="VFU15_06990"
     CDS             complement(2073..3224)
                     /locus_tag="VFU15_06990"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU4717559.1"
                     /translation="MPNTLSTGTGTDAVFFHATEGILVTDSRGEIIRINPAAERMFGY
                     EKGELTGKKIEILVPRRFTEKHVDHRDQFNRNPHSRRMGIGMALYGRRKDGSEFPVEV
                     SLSPYKSGEDTFTIAFIIDITIRKKQEEELEHLTRNLKESNAELENFAYISSHDLQEP
                     LRKIQSFGDRIRMTDGGHLSEKSRDHLDRLLNAASRMQNLINDLLMFSRLTTRAQDFR
                     PLNLNKVLEEVKSDIEVSVEKSGAQIVAVDLPTIDAEETQMRQLFQNLISNAIKFRKE
                     GETPVIEISSFKKKMTSADQHEMVEIRFRDNGIGFDQKYNDRIFNIFQRLDGKKYEGS
                     GIGLSICKKIALRHGGNITAESKPGEGAVFIVTLPVHQSTDKTNHHQHL"
     gene            3435..4814
                     /locus_tag="VFU15_06995"
     CDS             3435..4814
                     /locus_tag="VFU15_06995"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU4717560.1"
                     /translation="MADLSFSWWEADGVAYVLVVLITMSVSGILSTCIAGAAGVIFVI
                     VGCFVVPHENMHESAVMANRVLSCLGIAASTLLVIRYKRSAAELFRQKENLDAVFRFA
                     TEGIIVADEKGRIVRANPRTALQFGYSPGELPGKPVEDLMPARYRDKHAGQRSGFMHA
                     PSSRSMGERNVVLFGKRKDGTEFPVEIGLTSYKIREKRFAVAFVIDISERYRQQEELR
                     KTHLELKASNEELETFAFVASHDLQEPLRKIRSFSERLKDKEAERLSETGLDYLNRIA
                     NAGRRMQNLINDLLAYSRLTTRAKEFSVVDLDALLRDTLSDMEISIGESKAKINIVSP
                     LPSLLCDATQIRQVLQNLLSNAIKFRKPGVAPEITIGSQRDGKYPGYYAITISDNGIG
                     FDEKHTKKIFGFFERLENSKYEGTGIGLAVVKKIMSRHEGMIKVKSEPGKGSSFTLLF
                     PEEREEKGA"
     gene            complement(5053..>5506)
                     /locus_tag="VFU15_07000"
     CDS             complement(5053..>5506)
                     /locus_tag="VFU15_07000"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEU4717561.1"
                     /translation="IDAGRMAPSAMNQQPWKFYVVKDRKIIASISAAIRKMAESFFLF
                     SNGAELPESSDRIFYDAPLVIFLVGPKGDEWTALDIGMCAQNMMLAAKSLGLDTCPVG
                     FAKFIMQTSEYSRLKLNESEQVYLALAIGYGDETPKVHERIKENVIYI"
BASE COUNT         1200 a         1374 c         1485 g         1447 t
ORIGIN      
        1 atcttcgacc gtgtcgagct cgccggtgcc ggtaacgtta tcattgccga cgacctggaa
       61 aaggaattcc tccacgtgat gcacgcgccg gtgtgcgccg gatttccatt cttccacgta
      121 agctgctttc aggaacgcgg gcagctgtga aaggaaatgc agcgaagttt ccaccgggat
      181 atggtctctc atcgtgtgga gcacggcttt gaggatcctt cctgcttttt gcttttcgcc
      241 cgggaatccc aggacgtccg acaggtcatt caggaatgaa ttcccgtcat tgttcatttt
      301 ttcgaaattg aggctcatgg tcttttcttt ttgtggtaaa gatcatcgcc ccgcgcggct
      361 gtcccaatga tgccggtcaa aagaatattc cgaatgcgtc aggaaaaaat caggaaatgg
      421 aaacggggaa gctgatggag aagaccgtgc ctttgccgag ctcgctctgc atgtttattt
      481 ttccgtgcag cacttcaacg tatttcgcaa ggatgctcag gccgagtccc gttccctgga
      541 tgttggatac gttctgtccc cggaaaaaac gctggaaaag atgtttctgg tcttcctctg
      601 aaatgccgat cccgttgtcc ttcacttcga tcatcgcggt atcgttggcg caggatgttc
      661 tcacttcaat tttctttccg tcgtccgaaa acttgatcgc gttcgaaacg aggttgtgca
      721 ggatgttgcg cacgatcttt ttgtcggtgc tgacatcctc cgcatcgccg tcatgcgagt
      781 aaaggatctc ctggctgggt ttggcaatgc cgcgcatctc gcggatgatc tcttcggcga
      841 actgcatcag gctgaatgat tccggtgcgg ggacgatctt tccttcttcg agcttgctga
      901 gagaaagcac gtcgttgagc agctcggtca tgttggagac ggaagagccg atgcgtttcg
      961 tgtgcttctc cattttttcc gggtcgccga gctcagcata acgcttgacg agcgtgagcg
     1021 agctgagtat cgtggcgagc ggtgtgcgga attcatgcga agccatcgcg acgaagcgcg
     1081 acttgagatc gttcagctcc ttttcttttt cgagagcgac cgcgagctct tcctccgatc
     1141 ttttcagctc gcgcagtgcg ttctcgagcg tctgcgtgcg ttctttcacg cgttcctcca
     1201 gctctgcatt gagcttcgtg atcttcatca cgtttttatt ctgttcgata ctgtagcgga
     1261 tagcgcgttc cagctgtgaa gaagagatag agccctttac gaggaaatcg gctgcgcccg
     1321 cttcgagcgc catcgtatcg atcaccgggt cgctttgtcc cgtcatgaga atgaaaggcg
     1381 cttccacgcc catcgccacg ccttcacgga tcaggtcgat gccgttgttc acaccgaggc
     1441 ggtaatccac gagataaacg tcgtgttttt tttcaccgaa ggcgcgcagg ccatcctcgt
     1501 aggacgagac ccattcgagc gcaaaacgcc cgccggattt ttccagcagg atgtctttcg
     1561 tgatgatgaa atcttcctca tcgtcatcga tcatcaggat atgaatcttg tcttcgctca
     1621 tttttcgccg ccggaaggca gctccacgat ctcgaaccag tatttgttca gcgttttcat
     1681 tacatcgacc attgccgtga acgtcacggg tttcgtgatg aacgaattca cgccgaggtc
     1741 ataagagcgg agaatgtctt cttccgcctt ggaggtggtg agaacgatga tcgggatgct
     1801 gcgcagttcg tgcttctctt ttatttcgcg caaagcttcg cgtccgtctt tgcggggcat
     1861 gttgagatcg agaaggatca ggcccggctt gggatacttc tgcgtatcgg aaaattttcc
     1921 tttgttgttc aggtattcca tcagctcttc gccgttttcc actgtctgta tcttgttctt
     1981 cagccggctt tctttcagtg catcggtaat gagcatacgg tcttcctcat catcatccgc
     2041 gataaggatc tcgatagggt aaagaggatt tttcataagt gctggtgatg gtttgtttta
     2101 tcggtcgact ggtgtacggg caatgtgaca atgaacacgg cgccttcacc gggtttgctt
     2161 tcggcggtta tgttgccgcc atggcgcagc gcgatcttct tgcagatgga aagcccgatg
     2221 ccgctgcctt cgtatttctt tccgtcaagg cgttgaaaaa tattaaagat gcggtcattg
     2281 tatttctggt cgaagccgat accgttgtcg cggaaacgga tctccaccat ttcgtgctga
     2341 tcggcggagg tcatcttctt tttgaaagag gatatttcga tgaccggtgt ttcgccttct
     2401 ttcctgaatt tgatggcatt ggagatcagg ttctggaaca gctggcgcat ctgcgtttct
     2461 tccgcatcga tggtgggaag gtcgaccgcg acgatctgcg cgcctgattt ttcgacggaa
     2521 acttcaatgt cggatttcac ttcttccagc actttgttca ggttcagcgg gcggaaatcc
     2581 tgtgcgcggg tcgtcagccg ggagaacata agcaggtcgt tgatcaggtt ctgcatccgc
     2641 gaagcggcat tcagcaggcg gtcgaggtga tcacgcgatt tttcggaaag atgcccgccg
     2701 tccgtcatcc ggatgcggtc gccgaacgac tgtatcttgc ggagcggctc ctgcaggtcg
     2761 tgcgaagaga tgtaagcgaa attttcgagc tccgcgttcg attctttcag gttgcgcgta
     2821 aggtgttcca gctcctcttc ctgttttttc cgaatggtga tgtcgatgat gaacgcgatc
     2881 gtgaacgtgt cttcccctga tttatacggg ctgaggctca cttccacggg aaattcgctc
     2941 ccgtccttgc gacgtccgta aagcgccatg cctatgccca tcctccgcga atgcgggttg
     3001 cgattgaact ggtcgcggtg atccacatgc ttttccgtga aacggcgcgg aaccaggatc
     3061 tcgatctttt taccggtgag ctctcccttt tcataaccga acattctttc tgcggcagga
     3121 ttgatgcgga tgatctctcc gcgggaatca gtaaccagta ttccttctgt tgcatgaaag
     3181 aatacagcgt cggttccggt tccggtgctt agcgtgttcg gcattgatca cgtgaatatg
     3241 aaaattaaac gggtgtgtaa aaatagtaat taataaagca tgtatgtaca gaaacaacaa
     3301 gacggatgac gggaatcata aacagaatcc ttttcaacct gcatctttga tcgatttagt
     3361 cccccatggt aaatcaaaag agtaaaaatt tagtgctgac cttcttcgcc ggactggttg
     3421 ccatactttt tcttatggcc gatctcagtt tttcgtggtg ggaagcggat ggtgttgcgt
     3481 atgttctcgt ggttctgata accatgagtg tttcaggtat cctgtccaca tgcattgccg
     3541 gcgcagccgg ggtcattttt gtcatcgtag gatgtttcgt cgtgccgcat gagaatatgc
     3601 atgaatcggc ggtgatggcc aaccgggttc tttcctgcct cggcatcgcg gcttcaacgc
     3661 tgctggttat acgatataaa agatcggccg cggaactttt ccgccagaaa gaaaacctcg
     3721 atgctgtttt tcgttttgca acggaaggga tcattgtggc ggatgaaaaa ggccggatcg
     3781 tccgtgccaa cccgagaaca gctctgcagt tcggctattc gcccggggag cttcccggga
     3841 aaccggtgga agacctgatg ccggcgcgtt accgtgacaa gcatgccggg cagcggagcg
     3901 gtttcatgca cgcaccgtct tctcgttcaa tgggtgaacg taatgtggtc cttttcggga
     3961 aacggaaaga cggaacggaa ttcccggtcg agatcgggct gacgagctat aagatcaggg
     4021 aaaaacggtt tgctgtcgcc tttgtcatcg atatttcaga acggtaccgg cagcaggaag
     4081 aattgagaaa gacgcacctc gagctgaaag catccaacga ggaactggaa acattcgcgt
     4141 tcgtggcctc gcatgacctg caggaacctt tgcggaagat ccgctcgttc agcgagcggc
     4201 tgaaggacaa agaagcggag cggctgagcg aaacaggcct ggattacctt aatcgcattg
     4261 ccaacgcggg caggcgcatg cagaacctca tcaacgacct gcttgcatat tcgcgcctga
     4321 ctacgcgtgc gaaagagttt tctgtcgtcg acctggatgc gctgctgcgc gatacgcttt
     4381 cggatatgga aatttccatt ggcgaatcaa aggcgaagat caatatcgtg tcgcctcttc
     4441 cttccttgtt gtgtgatgcg acgcagatca ggcaggtgct ccagaatctt ctttcgaacg
     4501 cgatcaagtt ccggaaaccg ggtgtcgcgc cggagataac gatcgggtcg cagcgcgatg
     4561 ggaaataccc gggctattac gcgatcacca tcagcgataa cggcattggt ttcgatgaaa
     4621 aacatacgaa aaagatattc gggttcttcg aacgcctgga aaattccaaa tatgagggca
     4681 cggggatcgg actcgcagtg gtgaaaaaga tcatgtcgcg tcacgaaggg atgatcaagg
     4741 tgaaaagcga accgggaaaa ggttcttcgt tcacactgct tttcccggaa gagcgggagg
     4801 agaagggagc ctgagcggcg tttgttttac ggagctctgc gatttgaaaa acgttcacaa
     4861 caaggtatcg cacttccttt tttggttcgc tgcaattttc tttaaaaaag ttgcacaaaa
     4921 aagtgcgccg gatcggatcg ccttgtcatg ccgggcgcga gccgggatac tgcataaaac
     4981 tgttttgcga gggctgcaac aatggatcac tatgtcttgt cgggcgcgag tcgagacacg
     5041 acataaaacc attcagatat aaatcacatt ctcctttatc cgctcatgca ccttcggcgt
     5101 ttcatcaccg taacctatcg ccagcgcaag ataaacctgc tccgattcat tcagcttcag
     5161 ccgggaatat tccgatgtct gcatgatgaa ctttgcaaac ccgaccgggc aggtatcaag
     5221 tcccagcgat tttgcagcga gcatcatgtt ctgtgcgcac atgccgatgt ccagcgctgt
     5281 ccattcgtcg cctttcggac ccacaaggaa aataaccaaa ggagcatcgt aaaagatacg
     5341 gtcgctgctt tccggcaatt cagcgccatt tgaaaacagg aaaaaagatt cggccatttt
     5401 ccggatcgcg gcagaaatgg aagcgatgat cttccggtct ttcaccacgt aaaatttcca
     5461 gggttgctgg ttcatcgcgg aaggagccat acggccggca tcgatc
//
LOCUS       DASTSF010000242        10179 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1787563,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000242 DASTSF010000000
VERSION     DASTSF010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 10179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10179
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1787563"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2991
                     /locus_tag="VFU15_07005"
     CDS             <1..2991
                     /locus_tag="VFU15_07005"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HEU4717562.1"
                     /translation="RLGIPQGLSSDPVQAILTDSWGFTWIGTINGLNRFDGETFKLYE
                     HHSGDSASIPGDNVCSLAQDKNGELWVGTDNGIALFNPWNEEAVTIKCEQPAGKKVAP
                     PNHLNADALSGVVRAWSKDAAWIYNKDRKCFEPEIICSKQANGPDGCSGLYSDSENRL
                     WICSYDGLYVYSTHEKKITRHYQPLDASGKPVFFTGMIEVAPNDFWVTTWGSGFGRFT
                     ANDEQFHLYSWIDRPENPSASNIAFTLAAGKEHGRVIVWVGTNSGLFRIAANEFPSKD
                     NVTRLHNEPLNAESLPDDGVGCVEFNRYGQLWAGTGKGAGVILPQQQLFQPFAEQFRG
                     QTIRIVHDKGPGIFFCTWYGNGLQLCDSSGNILHAWKHVPENNPDIDCGQVSDVLRAS
                     DSTLWVATFGGLCHGDKNGKKFTQLLPSPNEKNSVCDRHIISLAEAPGGIFWFGSYGK
                     GVSRYDEKKNTWTNFRADSSGNSLCDNLVWDILCDREGRVWFATNTGISTYDEASGKF
                     RTWKNIIRGNDTIPFGIATSAFEDSRGVLWFTDDHGLFFIDRDGRAGMYGKDDGLSPG
                     AVTSINEDTRGILWICTTNGLSCFDPAKKIFTRYSITSGLPTNIMDGDISPLGGNMII
                     GLMNSVIIFNPVLLTQDVPANAPQLVSISLLGKKIGFSTSPAYAPELFYNWDQNTFTF
                     DFISLGFRGGPGVSYSYMLEGAENDWNYSGARHVASYGGLPPGSYHFLFRVSSGNGQW
                     SSPGSFSFTIRPPFWKTWWFITLSVVTLLALIVFIVRREATRKMRVRILQLEKLRAIE
                     KERNRISRDMHDDLGSGLTKIAILSEVAKKQVVNDSAKKQIDTISESARNLVDNLNEI
                     VWALNPENDNLQNLVAYIREYADHYLETFGIAFHCETPAEIPAVHLSEEKRRFLFLVV
                     KESLHNIVKHANATKVETLFSFADGKISIVIGDNGKGFEISEARPMGNGLKNMRKRME
                     SIGGMYTIASEPGKGTRTEINLSW"
     gene            3046..3672
                     /locus_tag="VFU15_07010"
     CDS             3046..3672
                     /locus_tag="VFU15_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007412948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU4717563.1"
                     /translation="MNIRVAIVEDTRDIREGLALLIDASDGFQCVDVCADGEEALEKL
                     PQSKPDVVLMDINMPGISGVECVKKLKPQLPQTQFMMCTVYEEDEHIFSSLKAGATGY
                     ILKKTAPGKLLEAIRDIHNGGSPMTGGIARKVIASLQPAPVDTDNGLSPREMEILEAL
                     AKGLRYKEIADRLFISIDTVRSHIRKIYEKLQVHSRTDALNKVFPKGM"
     gene            3955..5277
                     /locus_tag="VFU15_07015"
     CDS             3955..5277
                     /locus_tag="VFU15_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018341819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEU4717564.1"
                     /translation="MRPIEKAPPWFKDEYYSKAISLRKKDPRINDLIEKINQEYLYWD
                     RVKYQKAPGKISPEIIWILTKLSRVLSGKKLSFGKYKFTYNLTDIIQKGLHEFDLHIG
                     GDLGATTVVPAEDKNRYLVSSIMEEAIASSQIEGAVTTRRKAKEMLRKNIRPRNKSEQ
                     MIVNNYRTIKYIVDIQNEPITAERLFEIHRLIVNRTLEDPHDEGKYRSDNEVNVIDAA
                     DNEVVYTPPDFKEVASLMKDLFVFFNSNDEKQFIHPIIKGCIIHFMIGYIHPFVDGNG
                     RTARALFYWYLLKNGYWLTEYLSISRLIVKSKVQYAKAYVYTETDDNDLTYFLIYKIK
                     TMQLAYADLKSYIQSKIKEKKHLIAFQKIEGINERQAFILKWMHDEPDIILTIKEIET
                     RFNISNQTARTDLTTLQKMGYLETMGLDKKTKAFYKSAEFEKLLKKVL"
     gene            5384..6649
                     /locus_tag="VFU15_07020"
     CDS             5384..6649
                     /locus_tag="VFU15_07020"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEU4717565.1"
                     /translation="MKKSILISATLLPFFAGAQINVNGTLNDKANEKLGNAIDNVWSA
                     PGKMKDKKDGKNSGNSSDSTSNNSSNSNSGNSSSSSSSSSSSSSSSSSSSAAPTIKTY
                     QNYDFTAGSDLVFYDDFSEDMDGEFPSHWDLQAGQGVVNKIDGKPTFLLTDGNYVKVL
                     PVIDPKTYLSNSFTIETDVYAGAGDYGLRFFFMNGDDDQMDIQSANSEVTADFPKADG
                     SDEGSGLNGSLPQDVAYGSYDNHWHHIAIAYKDQQVKVYVDQNRVLVIPNCHCKPTSV
                     MFGGIGSTENPIKFTNVRIANGGNQNMLNTLMTTGKIISYGINFDVNKSDIKPESMGT
                     LNDIAKMMKDNPALKLAIGGYTDSDGDDASNLKLSQARADAVRTQLISMGIDGSRLTA
                     KGYGEANPIAPNTSFDGKAKNRRVEFTKQ"
     gene            6661..7671
                     /locus_tag="VFU15_07025"
     CDS             6661..7671
                     /locus_tag="VFU15_07025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717566.1"
                     /translation="MKRTLLLPVFLFAAALPAQVTLVQSQFPHAGDIFTLNMSDTTGV
                     LPGNSGTNVTWNFTSLNASQGVTTDSFMTVGSSLYGANYPQANLALHETGPSVDYFVY
                     YDVNSATADRVGNADSVNVITYTNPATQFYFPMSYGTTGSDTYYATYSDAASGSIVHV
                     HGTTSCNADGTGTLQLPASTYTNVLRAHFTRYENDTVFTTTSGAYPYQISTDYYLWYI
                     TGQYYPLLSIQSTVINIGFSPSYRKYVGWRNSPLDVPATATTSTLFAAPNPASSTLQL
                     AGPADGTLSFYAADGKLLMEKQVAKDEQLLVDVTGWEPGLVLVVFRNNEMTLRRSVMI
                     SR"
     gene            complement(7787..8659)
                     /locus_tag="VFU15_07030"
     CDS             complement(7787..8659)
                     /locus_tag="VFU15_07030"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEU4717567.1"
                     /translation="MDGTEKSITRLVLPGREEEISVLRLDLPDPVSGGNKFFKLKYNL
                     TAMREEGKEHLLTFGGAFSNHIAAVAAAGKREKIKTTGIIRGEELNIHSNEVLRFASS
                     CGMQLRFISRGDYRRREDPAFLAALKKQFDDPYMLPEGGSNAMAVKGCAEILSARTSS
                     FDAIFCPVGTGATLAGIISSAGKHQEIFGIAVLNGRSYLEEEVKKFLGERSVAARWRI
                     VHDYTAGGYARSSQGLEKFVHESSRTNALPLDHVYSGKALYAMHEMVKGDAFRGKQLL
                     FVHTGGYAFTASQR"
     gene            8743..8928
                     /locus_tag="VFU15_07035"
     CDS             8743..8928
                     /locus_tag="VFU15_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018278660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5522 domain-containing protein"
                     /protein_id="HEU4717568.1"
                     /translation="MKEKLPLEPGDFYHSEEGYIVFTEQYHLKRGYCCQSGCRHCPYG
                     YNKKTGRIEKKKGDKND"
     gene            8938..9495
                     /locus_tag="VFU15_07040"
     CDS             8938..9495
                     /locus_tag="VFU15_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016129672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU4717569.1"
                     /translation="MNFIFIFGPPAVGKMTVGQELGKLTGYRLFHNHVAIEPALLYFE
                     FGTPPFLRIVNTIRKSIFEEVIKAGMPGFIFTYVWALNEERDLHYVKQVRNFFAERGA
                     NVFFVELEADLSTRLERNRTENRLLHKASKRDTEWSEHNIRDMETKYVMNSEAFPFND
                     PYLRINNSQLSAPETAALIREKFQL"
     gene            9525..>10179
                     /locus_tag="VFU15_07045"
     CDS             9525..>10179
                     /locus_tag="VFU15_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HEU4717570.1"
                     /translation="MSSNDFHQLILEGIPEQLPAPKPFDASLNHAPKRKDILTASEKK
                     LALKNALRYFDKKHHAVLAKEFADELKKYGRIYMYRFRPDYKIFARPVHEFPHKSLQA
                     ACIMHMLSNNLDYAVAQHPHELITYGGNGAVFQNWAQYRLTMKYLATMTDEQTLVLYS
                     GHPLGLFPSHKDAPRVVVTNGMMIPNYSKPDDWEKFNALGVTQYGQMTAGSFMYIGPQ
                     "
BASE COUNT         2647 a         2775 c         2527 g         2230 t
ORIGIN      
        1 cgtctcggca tcccgcaggg attatcatcc gatcccgtac aggccatcct gacggattca
       61 tggggcttta cgtggatagg aacgatcaac gggctgaacc gtttcgacgg cgaaacgttc
      121 aagctttacg aacatcacag cggcgactcc gcttccattc ccggcgacaa cgtctgttcg
      181 ctggcgcagg acaaaaacgg tgagctttgg gtgggaacgg acaacgggat tgcgctgttc
      241 aatccctgga acgaagaagc ggttaccata aaatgtgaac agccggccgg gaaaaaagtg
      301 gcgccgccca accatcttaa tgccgatgct ctttccggcg tggtgcgcgc gtggagcaag
      361 gacgccgcct ggatttacaa taaagaccgg aaatgtttcg aaccggagat catctgctcg
      421 aagcaggcta acggacccga cggttgctcc ggtctctatt ccgattccga aaaccgtctc
      481 tggatctgtt cgtacgacgg cctgtatgtc tattcaacgc acgaaaaaaa aataacgcgg
      541 cattaccagc cgctcgatgc gtccgggaaa ccggtttttt tcaccggcat gatcgaagtc
      601 gcacccaatg atttctgggt gacgacgtgg ggttccggct tcgggcgttt cactgcgaac
      661 gatgaacaat tccacctcta ttcctggatc gaccggccgg aaaatccttc ggccagcaac
      721 attgcattca cactcgccgc cgggaaggaa cacgggcgcg tcatcgtgtg ggtgggcacg
      781 aactccgggc ttttccgcat cgccgcaaat gaatttccgt cgaaagacaa cgtcacgcgc
      841 ctgcacaacg aaccgctgaa tgcggaaagc cttcccgacg acggtgtggg gtgcgtcgag
      901 ttcaaccggt acgggcagct ttgggcgggc accggcaaag gcgccggcgt cattcttccg
      961 cagcagcagc ttttccagcc gttcgcggaa cagttccgcg ggcaaaccat ccgcattgtt
     1021 cacgacaagg gtcccggtat ttttttctgc acgtggtacg gcaacggttt gcagctctgc
     1081 gattcttcgg gaaatattct ccacgcctgg aaacacgttc ccgaaaataa tcccgacatc
     1141 gactgcggcc aggtgagcga tgtgctccgc gcatcggaca gcacgctatg ggtcgcgaca
     1201 ttcggcggac tttgccacgg cgacaagaat gggaaaaaat tcacgcagct tctcccttca
     1261 ccgaacgaaa aaaattcggt ctgcgaccgg cacatcatct cgcttgcaga agcgccgggc
     1321 ggaattttct ggttcggcag ctacggaaaa ggcgtgagcc gttacgatga aaagaaaaac
     1381 acgtggacga atttccgcgc ggattcatcc gggaattcgc tctgcgataa cctcgtctgg
     1441 gacatcctgt gcgaccggga aggacgcgtg tggttcgcga cgaacaccgg catcagcacg
     1501 tacgacgagg cttcaggaaa attccggacg tggaagaaca ttattcgcgg gaacgacacc
     1561 atcccgttcg gaattgctac atccgccttc gaagacagcc gcggcgtgct ctggttcacc
     1621 gacgatcacg gacttttttt cattgacagg gacgggcgcg ccggcatgta cggcaaggac
     1681 gacgggctga gcccgggagc tgtgaccagc atcaacgaag acacacgcgg aattctctgg
     1741 atctgcacta ccaacgggtt atcctgtttc gatccggcga agaagatttt cacacgctat
     1801 tccattactt ccggtctgcc gacgaatatc atggatggtg atatttcccc gctcggcggc
     1861 aacatgatca tcgggctcat gaattcggtc atcattttca acccggtttt gctgacgcag
     1921 gatgtccctg caaacgcgcc gcagctcgtc agcatctcgc tgctgggtaa aaagatcggt
     1981 ttcagcacat cgcccgctta tgcgccggaa cttttttaca actgggacca gaacacgttc
     2041 acgttcgatt ttatttcgct cgggttccgc ggcggccccg gcgtcagcta ttcatacatg
     2101 ctggaaggtg cggagaacga ctggaattat tcgggcgcac ggcacgttgc ttcatacggg
     2161 ggacttccgc cgggatccta ccattttctt tttcgcgtgt cctccggtaa cgggcaatgg
     2221 agcagtcccg gaagtttctc tttcaccatc cgtccgccgt tctggaaaac ctggtggttc
     2281 atcacgctga gtgtcgtcac gcttctcgcg ctcatcgtgt tcatcgtgcg gcgggaagca
     2341 acgcggaaaa tgcgtgtgcg catcctgcag ctcgaaaagc tccgcgcgat cgaaaaagag
     2401 cgcaaccgca tctcgcgcga tatgcacgac gacctgggtt cgggcctgac gaaaatcgcc
     2461 atcctcagcg aagtggcgaa aaaacaggtg gtgaacgaca gcgcgaaaaa acagatcgac
     2521 acgatctccg aatcggcgcg caaccttgtc gacaacctga acgagatcgt gtgggcgctc
     2581 aacccggaga acgacaacct gcagaacctg gtcgcttaca tccgcgaata cgccgaccac
     2641 tacctcgaaa cattcggcat cgcattccac tgcgaaacac ctgcggagat cccggccgtt
     2701 catctttccg aagaaaaacg caggttcctt ttccttgtcg tgaaagaatc gctgcacaac
     2761 atcgtgaagc acgcgaatgc cacgaaagtg gaaacgcttt tttcttttgc ggatggaaaa
     2821 atttccatcg tgatcgggga caacgggaaa gggttcgaga tatcggaagc gcgcccgatg
     2881 gggaatgggt tgaaaaatat gcggaagcgg atggaatcaa tcggcgggat gtacacgatc
     2941 gcttccgaac ccgggaaagg aacgcggacg gagattaacc tgtcctggta acaaccacat
     3001 gaaaatgtgg ccggaacatt tcaccgtgcg cttacatttg tacaaatgaa catacgcgtc
     3061 gccattgtag aagacacccg ggacatccgc gaaggactcg cgctcctcat tgatgcgagc
     3121 gatgggttcc agtgcgtgga cgtgtgcgct gacggggaag aagcgctgga gaaattaccg
     3181 caaagcaaac ccgacgtcgt gctgatggac atcaacatgc cgggcatttc cggcgtggaa
     3241 tgcgtgaaga agctgaaacc gcagctgccg caaacgcagt tcatgatgtg caccgtatac
     3301 gaagaggacg agcatatctt cagctccctg aaagccggcg ccaccgggta catcctgaaa
     3361 aaaaccgcac cgggcaaact gctggaagcg atccgcgaca tccacaacgg cgggtcgccg
     3421 atgaccggcg gcattgcacg gaaagtgatc gcttcacttc agcccgcgcc cgtcgatacg
     3481 gacaatggac tgagtccgcg tgaaatggaa atcctcgaag cgctcgccaa aggactccgt
     3541 tacaaagaga tcgccgaccg tcttttcatc agcatcgaca cggtgcgctc gcacatccgc
     3601 aagatctacg aaaagctgca ggtgcattcc cgcaccgacg cgctgaacaa ggtttttccg
     3661 aaggggatgt gaggttttcc tcgttttgtc cgcaacttaa aattctttca ccagattttc
     3721 caggttactt tttagttctg atgactctgt ctttttgatt tccaggggac tggaaaatca
     3781 aaaacgggct tttgaagtcg tttttttgaa caagggtaat accgtgatta tcagcacaat
     3841 gaattcgcgc tgttttataa aatgtcctga tttttgaaaa attttgacat tttttgaatt
     3901 ggcggtactt ttatagaaat caaaagcctt tcaaaagctt ttctcttttc gaaaatgaga
     3961 cctattgaaa aagccccgcc gtggtttaaa gatgaatatt attcaaaggc tataagcctg
     4021 cgaaaaaaag atccccggat caatgacctg attgaaaaga tcaaccagga atacctctat
     4081 tgggatcgcg taaaatatca aaaggcccca ggaaaaatca gtcctgaaat tatctggatc
     4141 ctgacgaagc tcagcagggt gctcagcggc aaaaaactga gtttcggaaa atataagttc
     4201 acatataatc ttaccgatat catccagaag ggtttgcacg aattcgatct ccacatcggg
     4261 ggagaccttg gtgctaccac ggtagttcct gctgaagata aaaacaggta tctcgtgagt
     4321 tcgattatgg aagaagcaat tgcttccagc cagattgaag gcgctgtgac aacccggaga
     4381 aaggcgaaag aaatgttgcg caaaaatatt cgtccgcgga acaaatcaga gcagatgatc
     4441 gttaacaact accgtacgat caaatacatt gtggatatcc aaaacgaacc aattacagcg
     4501 gaacggcttt ttgaaattca ccggttgatc gtaaatcgta cattggaaga tccgcatgat
     4561 gaaggaaaat acaggagtga caatgaagtg aacgtaattg acgcagccga caatgaagtg
     4621 gtgtacaccc ctcctgattt caaagaagtc gcatccctga tgaaagactt gtttgttttt
     4681 ttcaattcca atgacgagaa acaattcatt catcctatca tcaaaggatg catcattcac
     4741 ttcatgatcg ggtacattca cccgtttgtg gatgggaacg gaagaaccgc aagagctctt
     4801 ttctattggt atcttctgaa aaacggatac tggcttacgg aatatttgtc tatatccagg
     4861 ctcattgtta aaagcaaagt gcagtatgcc aaagcttatg tgtacacgga aacagacgat
     4921 aacgatctga cctattttct catttacaaa ataaaaacga tgcaattggc gtatgcggat
     4981 cttaaatcct atatccagag taaaatcaaa gagaaaaaac accttatcgc attccagaag
     5041 atcgaaggaa tcaatgaaag gcaagcgttc attctgaaat ggatgcacga tgaaccggac
     5101 atcatactga cgataaaaga gatagaaacc cggttcaaca tttccaatca aaccgcaaga
     5161 acggatctca caacactcca gaaaatggga tacctggaaa caatgggact ggataaaaag
     5221 acaaaagctt tttataaatc ggcagagttt gaaaaattgc tgaaaaaagt cctatagtag
     5281 ccgtccccgg cctgttccgc catccaaaac cgcattttca tgcgattgaa tgctacaggc
     5341 aactgcatga cttttgtatc gttcacacaa acgattcaaa atcatgaaaa aatccattct
     5401 catttctgca acactgctcc cgttttttgc cggggcgcag atcaacgtca acggcacgtt
     5461 gaacgacaag gccaatgaaa aactcggcaa cgcgatcgat aatgtctggt cggcgccggg
     5521 aaaaatgaaa gacaaaaaag acggcaagaa ttccggcaac agctccgaca gcacatcgaa
     5581 caacagcagc aacagcaatt ccgggaattc atcatcgtcc tcatcatctt cctcgtcatc
     5641 gtcctcctca tcctcttctt cgtcagccgc accgacgatc aaaacctacc agaactacga
     5701 tttcacggcc gggtccgatc tcgtctttta cgatgatttc tccgaagaca tggatggcga
     5761 atttccctcg cactgggacc tgcaggccgg gcaaggcgtc gtgaacaaga tcgacgggaa
     5821 accaaccttt cttctcaccg acggcaacta cgtgaaagtt cttcccgtca ttgatccgaa
     5881 aacgtacctc agcaattcgt ttaccatcga aaccgatgtg tatgccggcg caggagatta
     5941 cggtctccgc tttttcttca tgaacggcga cgacgaccag atggatatcc agtcggcgaa
     6001 ctctgaagta acggctgact ttccgaaagc cgacggcagc gatgaaggtt ccggcctcaa
     6061 cgggtcgctc ccgcaggacg tcgcttacgg gagttacgac aaccactggc accacatcgc
     6121 cattgcctat aaagaccagc aggtgaaagt gtacgtcgac cagaatcgcg tgctcgtaat
     6181 tcctaattgc cactgcaagc cgacttccgt aatgttcggc ggcatcggct cgaccgaaaa
     6241 cccgatcaag ttcacgaacg tgcgcatcgc caacggcgga aaccagaaca tgctgaacac
     6301 actgatgacg accggcaaga tcatttccta cggcatcaat ttcgacgtga acaaatccga
     6361 catcaaaccc gaaagcatgg gcacgctgaa cgacatcgcg aaaatgatga aagacaatcc
     6421 cgcgctgaag ctcgcgatcg gcggctacac cgacagcgac ggagacgatg catcgaacct
     6481 gaagctttcc caggcccgcg ccgacgccgt gcgcacgcag ctcatctcga tgggcatcga
     6541 cggttcgcgc ctcacggcaa aaggatacgg cgaagccaat cccattgcgc cgaacacttc
     6601 cttcgacggg aaagcgaaga accgccgcgt ggagtttacc aaacagtaat aatccatact
     6661 atgaaacgaa cactgttgtt gcccgttttt ttatttgcag ctgcattgcc tgcgcaggtg
     6721 acgctggtcc aatcgcagtt tccccatgcc ggtgatattt tcaccctgaa catgtccgat
     6781 accaccggtg tgctccccgg caacagcggg acgaacgtta cgtggaactt cacttcgctc
     6841 aacgcttcgc agggcgtcac tacagattca ttcatgaccg ttggctcctc cctgtatggc
     6901 gccaactacc cgcaggcgaa ccttgcgctg catgaaacgg gaccgtcggt cgattacttc
     6961 gtttattacg atgtcaattc cgcaacagca gatcgcgtcg ggaatgccga ttcggtgaac
     7021 gtcatcacct ataccaatcc cgcaacacaa ttctatttcc cgatgagcta cggcaccacg
     7081 ggcagcgaca cgtattacgc cacgtattcc gatgccgctt cgggaagcat tgtgcatgtg
     7141 cacggcacca ccagctgcaa cgccgacgga acggggacac ttcagctgcc ggcttcgacc
     7201 tataccaacg ttcttcgcgc acatttcacg cggtacgaaa acgacacggt gttcacgacc
     7261 accagcggcg cttatcccta ccagatcagc accgactatt atctctggta catcacggga
     7321 caatattacc cgctgctgag catacagtcg accgtgatca acatcggctt ctccccttcg
     7381 taccgcaaat atgtcggctg gagaaattct ccgctggatg ttccggccac tgcgacaaca
     7441 tccacgcttt ttgcagcgcc caatcccgca tcatcgaccc tgcagcttgc aggtccggcc
     7501 gacggaacac tgagctttta cgctgccgac ggaaagctgc tcatggaaaa gcaggtggcg
     7561 aaagacgaac aattgctcgt cgacgttacc ggttgggaac cgggacttgt gctcgttgtt
     7621 ttcaggaaca atgaaatgac gctgcgccgt tctgtgatga tctcacggta acggtctgcc
     7681 ggaccccgtg cgtgcgattt tctttcaccg gaaaaacgca taatgacaaa cctgccattt
     7741 ctccataccg gggacgcatg ttttcaggta agctgccctg tgcagatcag cgctgacttg
     7801 cagtgaaagc atagcccccg gtgtgaacaa aaaggagctg ttttccacgg aaagcatctc
     7861 cttttaccat ttcatgcatg gcgtagagcg ctttcccgga ataaacatga tcgaggggca
     7921 gcgcatttgt gcggctgctt tcgtgaacaa atttttcaag tccctgcgag gaacgcgcat
     7981 acccgcccgc cgtataatcg tgcacgatgc gccatcgggc ggcgacactg cgttcgccga
     8041 ggaatttttt cacttcttct tccaggtaag accttccgtt cagcacggcg atgccgaaga
     8101 tctcctggtg ctttcctgca gaagaaataa ttcccgccag tgtcgctccc gttcccaccg
     8161 ggcagaagat cgcatcgaag gacgaggtgc gggcactgag aatttccgcg cagcctttca
     8221 cggccatcgc gttcgatccg ccttcgggaa gcatgtacgg atcatcgaac tgctttttca
     8281 gcgcagcgag gaacgcagga tcttcccttc tccggtaatc gccgcgcgaa ataaaacgga
     8341 gctgcattcc gcaggacgaa gcgaagcgca ggacttcatt cgagtgaatg ttcagctctt
     8401 ccccgcgaat gatgccggtg gtttttattt tttctctttt ccccgccgcg gccacagcag
     8461 caatgtgatt cgaaaaggcg ccgccgaagg tgagcaggtg ctctttccct tcttcacgca
     8521 tcgcggtgag attgtatttc agcttgaaga atttgttgcc gccggaaaca ggatccggaa
     8581 gatcgagccg gagcacggaa atttcctcct ccctcccggg aagaacaagt cgtgtaatgc
     8641 ttttttccgt tccgtccata cgccttgcag ccgcggtttc cgtctctttt cccaaaatac
     8701 cactttctgc cttcacgcaa aaggtttgaa taactttgtc gcgtgaaaga aaagcttccc
     8761 cttgaacccg gcgattttta ccacagcgag gaaggataca tcgtttttac cgaacagtat
     8821 cacctgaaac gcggctattg ctgccagagc ggatgcaggc attgtcctta cggttacaat
     8881 aaaaaaacgg gaaggatcga gaaaaagaaa ggcgataaaa acgactgaac cgctttcatg
     8941 aatttcattt tcatattcgg accacccgct gtcgggaaaa tgaccgtggg acaggagctg
     9001 ggaaagctta ccgggtacag gctctttcac aatcatgtcg cgatcgaacc tgcgctgctt
     9061 tatttcgaat tcggaacgcc gcctttcctg cgaatcgtga acaccatccg caaaagcata
     9121 ttcgaagaag tgatcaaagc gggaatgccc ggtttcattt ttacgtacgt ctgggccttg
     9181 aatgaagaac gcgacctgca ctacgtgaag caagtgcgga attttttcgc agaacgcggt
     9241 gcaaatgttt ttttcgtgga actcgaagcc gatctttcca cgcgtctcga acgcaaccgg
     9301 acagaaaacc gcctgcttca caaagcgagc aagcgcgaca cggaatggtc agagcacaac
     9361 atccgcgaca tggaaacgaa atacgtgatg aactccgaag cttttccgtt taacgatcct
     9421 tacctgcgca tcaacaattc tcaactttcc gcaccggaaa cagcagcgct tatccgggaa
     9481 aagtttcaac tttgaaccac cagtcgccag ccattccaca cactatgtcc tcaaacgatt
     9541 ttcaccagct cattctcgaa gggatcccgg aacagctccc cgctcccaaa ccgttcgacg
     9601 cctcactcaa tcacgcaccg aagcgaaaag atattctcac tgccagtgaa aaaaagctgg
     9661 cgctgaaaaa tgcgcttcgc tatttcgata aaaaacacca tgcggtcctc gctaaagaat
     9721 tcgcggacga gctgaaaaaa tacgggcgca tttacatgta ccgcttccgc cccgattaca
     9781 agatattcgc acggcctgtg cacgagttcc cgcacaaaag cctgcaggcc gcctgcatca
     9841 tgcacatgct gtcgaacaac ctcgactacg ccgtggcgca gcatccgcat gagctcatta
     9901 cgtacggcgg caacggcgct gtatttcaga actgggcgca gtaccgcctc acgatgaaat
     9961 acctcgcgac gatgacagat gaacaaacgc tcgtcctgta ttccggtcac ccgctcggac
    10021 tttttccttc gcacaaagat gcgccgcgtg tcgtggtgac gaacggcatg atgatcccga
    10081 attattcgaa gccggacgac tgggaaaaat tcaatgcgct cggcgtgacg caatacgggc
    10141 agatgacggc cggatcattc atgtacatcg gtccgcagg
//
LOCUS       DASTSF010000243         4082 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1271341,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000243 DASTSF010000000
VERSION     DASTSF010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4082
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1271341"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1584
                     /locus_tag="VFU15_07050"
     CDS             <1..1584
                     /locus_tag="VFU15_07050"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HEU4717571.1"
                     /translation="RINAHAQFPTVKWQYDIGAPSFGSAAAADLDKDGFDEIVFSTYT
                     ADGRVHCLNAEDGSERWVYNIGGCGDVAPLICDVDNDDTLDVIVNGSCNPTIFCINGY
                     TGHLKWSKPSGGGDSPPTVTDIDHDGLPEILFCNFNGELRILNGEDGSTNRVVQVDPG
                     YNPIETEPTLVDANNDSTPDVIMANYTNVNGLYIWAYNYVTNDTLWTDYLPDSASFAY
                     YHGGAVADVDGDGKPEYVIGSNSGRVRALNTEDGSQLWSVMIPASDMAPVSIADLDHD
                     GNPEVVVTNNDWITYDERIWVLDGATGNTEWSFPTTFSSFRGCAIADINGNDTLDLVA
                     GFYMGDVIAVEPYTGLIWQMNLMSYFSPGLPWFDVDHGPLVADFDHNGKMDVFVVAGY
                     GTYTPDSMNTGKAFMIEAGNGHCPEWLMFRQDVYRSGYLSPQDESASCSAVQGIPADA
                     KDPFAFSVTPDPAEDETRISLPGHFSVEVYDLSGRRMYIRMQNQDACTLDVSGWTPGM
                     YIVKVRQDGKEADRKLVVE"
     gene            complement(1613..2512)
                     /locus_tag="VFU15_07055"
     CDS             complement(1613..2512)
                     /locus_tag="VFU15_07055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717572.1"
                     /translation="MKIVKEKKLPLLPGTPVTALALLPGQQVLVSGQDSQDGEQPNVC
                     CWDLRTKKLAASFFSESYTRVNAVSPGNGPLSVIFPASENVLAAGDLRSTTQTDFYSP
                     VSCVRKLAWSEKIQQLLVSGDKTVCIDAAGKQLWSMPGDHERDPVYPTPPLIDWTGDN
                     ERTAFMGQGVRAGVYEGRQLLRPFEGAPEKIISLSCNDKLTGAISYPGNQLRFWNHDG
                     SEYLPGVFAEKKVKSFAFGNGHLLLCGTIAGHVQGYDIRSGERLFSQEIHKRGAVSAL
                     ACCDDLVISGGQDGTLWLYRIEE"
     gene            complement(2517..>4082)
                     /locus_tag="VFU15_07060"
     CDS             complement(2517..>4082)
                     /locus_tag="VFU15_07060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717573.1"
                     /translation="GEKVVAGLEKTFTLVKTLVTEGPMAAWEQLKEIAGDMRDAFIDA
                     VKDFIATKIIEQAITWLVSIFVPGAGIIKAIIGIYDTIVFFIQKAKEIMQMIGNFLGS
                     IAEIAAGNIGAAADALENGLARGLSLVINFLAKLLHLDGITSKIRNAISKIRAKVDAV
                     IEKVAKWVAEKAKKIWGKITGKEEPKKEGADSAAGDATPISFEVKEPFSMDGKSHHLI
                     LQVKANTPSLLIASDPALLIPALENAMTEVRSSSRTATEKADILDWLERAHKEAKDVP
                     FTVKARINDVLKGKPHEHKAEQEAIAREEAKELAQIANVLFTLSRKWNVKALDDFFKA
                     PPTRRYIPGSSQTEIGKFIRGRLYENMHDWSSASKSFKTAEFPGLKTKVLAIQSDAVA
                     AKNKGNKAGLQIAKDKWQDAIDNGLILKNADIFTHTPDVSDYHADHDPPLTDRWNAGG
                     NDGDDNLREKQVKGNLVLMTSEKNLSLGSRGGNYDPFVLPRFTSSIADGGIVYARSID
                     GKRFVDAAKKPIT"
BASE COUNT          833 a         1070 c         1143 g         1036 t
ORIGIN      
        1 cgcattaatg cgcatgccca gttccccacc gtaaaatggc agtacgatat cggcgcgcct
       61 tcgttcggtt ctgctgccgc tgccgatctc gacaaagacg ggttcgatga aattgttttc
      121 agtacctata cggcggacgg ccgcgtacat tgtctcaatg cggaagacgg atcggagcgg
      181 tgggtgtaca atatcggtgg ttgcggtgat gtggcgccgc tgatttgcga tgttgacaac
      241 gacgatacgc tcgatgtcat cgtgaacggt tcatgcaatc ccaccatttt ctgtataaat
      301 ggttataccg gtcatctcaa atggagcaaa ccttccggtg gcggcgattc tccgccgacc
      361 gttacggata tcgatcatga cggcctgcct gaaattcttt tctgcaattt caacggagag
      421 ctgcgtattc tcaacggcga ggacggaagc acgaaccggg ttgtgcaggt cgatccgggt
      481 tacaacccga ttgaaacgga accgacgctt gtcgacgcga ataacgacag tacgcctgat
      541 gtcatcatgg cgaattatac caacgtgaac gggctgtata tatgggcgta taattatgtc
      601 accaatgaca ccctgtggac tgattacctg ccggattcag catcgttcgc gtattatcac
      661 ggcggtgctg tggcggatgt ggacggtgat ggaaaacctg aatacgtgat aggttccaac
      721 agcggccgcg tgcgtgcact gaacacagaa gacggatcgc agctgtggtc ggtgatgata
      781 cccgcgagcg acatggcgcc ggtctcgatc gctgatctcg atcacgacgg caatccggaa
      841 gtagtggtga cgaacaacga ctggatcact tatgacgagc gcatctgggt gctcgacggc
      901 gcaacgggaa atacggaatg gtcgttcccg acgacgttca gctcgttccg cggctgcgcc
      961 attgcggata tcaacgggaa cgatacgctg gatcttgtcg ccggatttta catgggcgat
     1021 gtgatcgccg tggaaccata taccggattg atctggcaga tgaacctgat gagttatttt
     1081 tcacctggcc ttccatggtt cgacgtcgat catggtccgc tggttgccga tttcgatcac
     1141 aacgggaaga tggacgtgtt tgtcgtggcc ggttacggaa cctatacgcc cgattcgatg
     1201 aacaccggga aagcgttcat gatcgaagca ggcaacggac actgtccgga gtggctgatg
     1261 ttccggcagg acgtttaccg gagtggttat ttatcaccac aagatgaaag cgcgtcgtgc
     1321 agtgccgtgc agggaattcc ggcggatgcg aaagatccgt tcgcattttc cgtgacgcct
     1381 gatcctgcag aagatgaaac gcgtatttcg ttgcccggtc atttttccgt tgaagtatac
     1441 gatctgtcgg ggagaagaat gtacatccgt atgcaaaacc aggatgcctg cacgctcgac
     1501 gtgagtgggt ggacacctgg catgtatatc gtgaaggtgc ggcaggacgg gaaagaagcg
     1561 gaccggaagc tggtggtgga gtgatttttt atcgcagaaa ccggttgttc ggttattctt
     1621 cgatacgata gagccacagg gtgccgtcct gtccgccgga aataacaaga tcgtcacaac
     1681 aggcgagtgc gcttacggct ccgcgcttgt ggatctcctg tgaaaacagc cgttctcccg
     1741 agcgtatgtc atagccctgc acatgcccgg ctattgttcc gcagagaaga aggtgtccgt
     1801 tcccgaatgc aaatgatttc actttttttt ctgcgaacac cccgggaaga tattcgcttc
     1861 cgtcgtgatt ccagaagcgc agctggtttc cggggtagga tatggcgccg gtaagcttgt
     1921 cattacagga aagagaaatg attttttccg gtgcaccttc aaatggtctc agcagttgcc
     1981 gtccttcata aaccccggcc ctgactccct gtcccataaa agcggtgcgt tcattgtcgc
     2041 cggtccagtc gatcaatggc ggggtaggat agacaggatc cctttcgtga tcaccgggca
     2101 tgctccacaa ttgtttcccg gcggcatcga tgcaaacggt tttatctccc gacacaagca
     2161 gctgctgtat tttttccgac catgcgagtt tgcgcacgca gctcaccggg gaatagaaat
     2221 cggtttgcgt ggtgctccgc agatcgcccg cggcgagtac attttcgctg gccgggaaga
     2281 ttaccgaaag cggtccgttg cccggggaaa cggcgttcac cctcgtatag ctttcggaaa
     2341 aaaaagatgc ggccagcttt ttcgtccgca gatcccagca acacacgttc ggctgttcgc
     2401 catcctgtga atcctggcct gaaacaagaa cctgctgccc gggcaatagg gcaagcgccg
     2461 ttacaggtgt gccgggcagg agcggcagct ttttttcttt gacgattttc atcgcatcaa
     2521 gttattggtt ttttcgcggc gtcgacaaaa cgttttccat ctatgctgcg tgcataaaca
     2581 atccctccgt ctgcgatgct gctcgtaaac cggggaagga caaaaggatc gtagttcccg
     2641 ccccttgaac cgagcgaaag atttttttca gaggtcatca gcacaagatt gcctttcacc
     2701 tgtttttcgc ggagattgtc atcgccgtca ttcccgccgg cattccaccg gtcggtgagc
     2761 ggcggatcgt gatcggcatg gtaatcggac acgtcgggtg tatgcgtgaa aatatcggcg
     2821 ttcttcagga tcagcccgtt gtcgatagcg tcctgccact tgtccttcgc gatctggagg
     2881 cccgccttgt ttcctttgtt ctttgcggcg accgcatcgg actgtatcgc gaggactttt
     2941 gttttgagtc ccgggaattc tgccgtcttg aatgatttgc tggcggaaga ccagtcgtgc
     3001 atgttttcgt ataaccgtcc gcggatgaac ttgccgattt ccgtttggct gctgcccgga
     3061 atgtatcgcc gcgtaggagg ggctttgaaa aaatcgtcga gcgcctttac attccatttg
     3121 cgggaaagcg tgaaaagcac attcgcgatc tgtgcaagct ctttcgcttc ctcgcgcgca
     3181 atggcttcct gctcggcttt atgctcatgc ggcttgcctt tcaggacgtc attgatgcgg
     3241 gctttgacgg taaacggaac gtctttcgcc tctttatgcg cacgttccag ccagtcgaga
     3301 atgtccgctt tttccgtcgc tgtccgtgac gatgatctta cttcggtcat ggcattttcc
     3361 agggcaggga taagtaatgc gggatcgctc gcgatcagga gcgatggtgt gttggctttt
     3421 acctgcagga tcaggtggtg cgatttcccg tccatcgaga acggttcttt cacttcgaag
     3481 ctgatcggcg tcgcgtcccc ggccgcgcta tccgcgccct cttttttcgg ctcttctttt
     3541 cccgtgatct tcccccatat cttcttcgct ttttcagcga cccacttcgc cactttttcg
     3601 atcacggcat ccactttcgc ccggatcttg ctgatggcgt tgcggatctt cgatgtgatg
     3661 ccgtcgaggt gaagcagttt tgcaaggaaa ttgatcacga gcgaaagtcc gcgcgcgagc
     3721 ccgttctcga gtgcgtctgc cgcggcgccg atatttcccg cggcgatctc tgcgatggag
     3781 ccgaggaagt tgccgatcat ctgcatgatc tctttggctt tctggatgaa gaaaacgatc
     3841 gtatcgtata tgccgatgat ggccttgatg atgcctgcgc cgggaacgaa gatgctgacc
     3901 agccacgtga tcgcctgctc aatgatcttc gtggcgataa aatctttcac ggcatcgatg
     3961 aacgcgtcgc gcatgtcgcc ggcgatttct ttcagctgtt cccacgcggc catcggcccc
     4021 tcggtcacca gtgttttgac aagcgtgaat gttttttcga gaccggcgac gaccttttcg
     4081 cc
//
LOCUS       DASTSF010000244         4737 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_38573,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000244 DASTSF010000000
VERSION     DASTSF010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4737
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38573"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(80..427)
                     /locus_tag="VFU15_07065"
     CDS             complement(80..427)
                     /locus_tag="VFU15_07065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717574.1"
                     /translation="MKRNTGALYFLCSRFSIGANGFTASLLIPGKQPLKPDQNLRETT
                     SDLKHHATLRELCEKNFVRFVVKKPRRTQRNGNGHDAGVVKQLRLSRIKIPVHLCEKT
                     SVNLRVKTSRLAW"
     gene            complement(438..914)
                     /locus_tag="VFU15_07070"
     CDS             complement(438..914)
                     /locus_tag="VFU15_07070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717575.1"
                     /translation="MSVPVRIGIIGGIIIGLLQFYTLIACPPGFIGGIIFYSPQILYF
                     ACIYLSIRKTKAAGEASFKGGMKAGVITAFFIAIIWGIGFFIGMNNIDVHGQALYALA
                     HGTSKADVLAILKGWTQDAMIERTKFWMIPNFLLGFVVSLAVTIILRRKNPLNPEG"
     gene            complement(914..2794)
                     /gene="rho"
                     /locus_tag="VFU15_07075"
     CDS             complement(914..2794)
                     /gene="rho"
                     /locus_tag="VFU15_07075"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005652729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEU4717576.1"
                     /translation="MYDIIELNSKLVGELREIARQLNIPKHDTLKKQELIYKILDQQA
                     VSGVKKEAEDTSDSSKPRRGRKPKPDSAQEEPELFTGTQKEDHRFVSIGNETNTAEPK
                     KEEPEVKVNAGNVTPEPKKPENRPVEMPRQNQHGQHRKPFHQKTPYNKPNTPPPPPPA
                     YNQPPAFIQAQMNKQDNFQKPEPNISAPQNQPQNMQQNPPQNQNPSQNQPSQQQPQQQ
                     QQQPQQQQHQNYQQPGNFQRQNYQQKPPPEEMYNFDNVVVAEGVLEIMPDGYGFLRSS
                     DYNYLNSPDDVYVSQSQIKLFGLKTGDTVRGAIRPPKEGEKYFPLVKVDKINGRDPGY
                     VRDRVPFDFLTPLFPNEKFKLAGSGKSKTNMSMRVMDMFTPIGKGQRGLIVAQPKTGK
                     TILLKEVANAIADNHPEAYLMILLIDERPEEVTDMARSVRAEVIASTFDEPADRHVKI
                     ANIVLEKAKRMVECGHDVVILLDSITRLARAYNTVQPASGKVLSGGVEANALQKPKRF
                     FGAARKIEGGGSLTIIATALIDTGSKMDEVIFEEFKGTGNMELQLDRKLANKRIFPAI
                     DLVSSSTRRDDLLMDKDMMQRIWILRNHLADMNPQEAMEFLKDRMKNTQSNEEFLISM
                     NG"
     gene            3052..3462
                     /locus_tag="VFU15_07080"
     CDS             3052..3462
                     /locus_tag="VFU15_07080"
                     /inference="COORDINATES: protein motif:HMM:NF025491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4293 domain-containing protein"
                     /protein_id="HEU4717577.1"
                     /translation="MIQRIQSLFLLFPSIIFATLFFVPVFTVDGTPVFMIGSRPETLA
                     IGAIALLGLVALFMYRNRKRQMRMCRAGIILSVLYSANLFVFPKWLLGNAAASVVSTG
                     PGSWLLVSNVIFFVLALIFIRKDDELVRSADRLR"
     gene            complement(3737..>4737)
                     /locus_tag="VFU15_07085"
     CDS             complement(3737..>4737)
                     /locus_tag="VFU15_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008508984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU4717578.1"
                     /translation="VEILVSVSLGLMVWFAGKRVYSGFDGPGDIAMFVILINQFFRPI
                     RMLADRINTLQMGMVSSERVFKVMDTEAAIDPVENGSTEKIRGEVVFEDVWFAYKNED
                     WVLKGISFGVKPGETIALVGATGSGKTSIINLVNRFYEFQKGSIRIDGKDIRSYNLKN
                     LRSHMGIVLQDVFLFSDTIFNNITLYNPEISRERVIAASKAVGAHDFIMQLPGDYDYD
                     VRERGGTLSVGQRQLIAFIRAYVYDPTILVLDEATSSIDTESEILIQRATKTLTKGRT
                     SIVIAHRLATIQNADRILVIDHGQIIESGSHQQLLAMGGHYKKLFELQFRDEEVVK"
BASE COUNT          970 a         1161 c         1349 g         1257 t
ORIGIN      
        1 cgaatttttt actgctgcaa ttttctttgt gtccttcgtg tttttttacc acaaagcgca
       61 caaagttttt tcagagagtt caccaagcaa gacgtgatgt tttgacgcgg aggttcacgg
      121 aggttttttc acagagatgc accgggattt tgatccgact taaccttaac tgcttcacca
      181 ccccggcatc gtgaccatta ccatttcttt gtgtccttcg tggtttttta accacaaagc
      241 gcacaaagtt tttttcgcag agttcacgaa gcgtggcgtg atgttttaag tcggaggttg
      301 tttcacggag attttgatcc ggttttaacg gctgttttcc gggaatgagc agcgatgcag
      361 tgaaaccatt agccccgatt gaaaagcggg agcagaggaa atacagcgca ccggtgttgc
      421 gcttcaaaaa ataaagatca gccttccggg ttcagcgggt ttttcctgcg caggatgatc
      481 gtcacagcca ggctgacgac aaaaccgagg aggaaattcg ggatcatcca gaatttcgtg
      541 cgctcgatca ttgcgtcctg cgtccagcct ttgagaatgg ccagcacatc cgctttcgac
      601 gttccgtgcg caagcgcgta gagcgcctgc ccgtgcacgt cgatgttgtt catgccgatg
      661 aaaaatccga ttccccagat gatggcgatg aaaaaagcag tgatgacacc ggctttcatg
      721 ccacctttga agctggcttc gccggcggct tttgtcttgc ggatgctcag gtagatgcag
      781 gcgaaataca ggatctgcgg actgtagaaa atgatgccgc cgataaagcc gggagggcag
      841 gcgatcagcg tgtaaaactg cagcagcccg atgatgatcc cgccgatgat gccgatgcga
      901 accggaacgc tcatcagccg ttcatggaaa tgaggaactc ctcgttgctc tgggtgttct
      961 tcatgcggtc tttcaggaat tccatcgctt cctgcgggtt catgtccgcg aggtgattgc
     1021 gcaggatcca gatgcgctgc atcatgtctt tgtccatgag caggtcgtcg cggcgcgtgg
     1081 aagaagaaac gagatcgatg gccgggaaga tgcgcttgtt cgcgagcttg cggtcgagct
     1141 gcaattccat gttgccggtt cctttgaatt cttcgaagat gacttcgtcc attttcgaac
     1201 cggtatcgat gagcgcagtg gcaatgatcg tcagcgatcc gccgccttcg atcttccgtg
     1261 ccgcaccgaa gaaacgtttc ggcttctgga gcgcgttcgc ttcgacgccg ccggaaagca
     1321 ctttgccgga agcaggctga acggtgttgt aggcgcgtgc aaggcgcgtg atggaatcga
     1381 ggaggatgac gacgtcgtgc ccgcactcga ccatgcgttt cgctttttcg agcacgatgt
     1441 tggcgatctt cacgtggcgg tcggccggtt catcgaacgt ggaagcgatg acttccgcgc
     1501 gcacgctgcg ggccatgtcg gtgacctctt ccgggcgctc gtcgatgagg aggatcatca
     1561 ggtacgcttc cgggtgattg tcggcgatgg cgttggcgac ttccttcagc aggatcgtct
     1621 taccggtttt cggctgcgcc acgatgagtc cgcgctggcc tttaccgatc ggcgtgaaca
     1681 tatccatgac gcgcatcgac atgttcgttt tcgactttcc gcttcccgcg agcttgaatt
     1741 tttcattcgg gaaaagcggt gtgaggaaat cgaaaggaac gcggtcgcgc acgtagccgg
     1801 gatcgcgccc gttgatcttg tccactttta cgagcgggaa atatttttca ccttctttcg
     1861 gcgggcgaat ggcaccgcga acggtgtcgc cggttttgag accgaagagc ttgatctgcg
     1921 actgggagac gtaaacgtcg tcgggcgagt tgaggtagtt gtaatcagaa gagcgaagga
     1981 aaccgtaacc gtcgggcatg atctcgagca caccttccgc gacgacgaca ttgtcgaagt
     2041 tgtacatttc ttccggcgga ggtttctgct ggtaattctg ccgctggaaa tttccaggct
     2101 gctggtagtt ctggtgctgc tgctgctggg gttgttgttg ctgttgctgg ggctgctgct
     2161 gggaaggttg attctgggaa ggattctggt tctgcggagg gttctgctgc atgttctggg
     2221 gctggttctg cggagcggaa atattcggct cgggtttctg gaaattatcc tgcttgttca
     2281 tctgtgcctg gataaatgcc gggggctggt tgtacgccgg gggaggcggg ggaggtgtat
     2341 tcggcttgtt gtagggcgtt ttctggtgga acggcttgcg gtgctgcccg tgctggttct
     2401 ggcggggcat ttcaacgggc ctgttttccg gttttttcgg ttcgggcgta acgttgcccg
     2461 cgttcacttt cacttccggc tcttctttct tcggttccgc ggtgttcgtt tcattcccga
     2521 tggaaacgaa gcggtgatct tctttctggg ttccggtaaa gagttccggc tcttcctgtg
     2581 cagaatcggg cttcggtttt cttccgcgcc tgggttttga cgaatcggac gtgtcttccg
     2641 cttctttttt cacgcctgaa acggcctgct gatcgaggat cttgtagatc agctcctgct
     2701 ttttcaatgt gtcgtgtttg gggatgttca gctgtcgcgc aatttcacgc aattcaccga
     2761 ccagcttgct gttcagctcg ataatgtcgt acatgagatg gattaagtga gaaaattttt
     2821 ttctggttgt tgaggaagtt gcactgcgcg tgtggaagga aaaaagcgcc cgcaaaaggt
     2881 ttagttcagc atcagtattt catcttgttc aggggaaagg aaactgaaag tgcttcctga
     2941 agaattctgt gcaataatac gaagattatt tggttccaaa caaaaggcaa aaataatttt
     3001 ccacattgta aggcacgccg ggaatgggtt attttgcctg aaaataaatt tatgatccag
     3061 cgcatccagt cacttttcct gctctttccg tccatcattt tcgcgacact gtttttcgtt
     3121 cccgtgttca ccgtcgacgg aacgccggtg ttcatgatcg gctcgcggcc tgaaaccctg
     3181 gcgatcggtg cgatcgcgtt gctcgggctt gtggcgcttt tcatgtacag gaaccgcaaa
     3241 cgccagatgc ggatgtgccg tgcggggatc attctttccg tactttattc cgctaacctg
     3301 tttgtttttc cgaaatggct gctgggaaat gcagcggctt ccgttgtttc gaccgggccc
     3361 gggtcgtggc tgctcgtttc caatgtgatc ttcttcgtgc tggcgctgat cttcatccgt
     3421 aaagacgacg agcttgtacg ctcggcagac aggttgcggt gaaatacgtt cactgtttcg
     3481 ggtttaaagt tgaaagttga aaagttaggg tagaagaggt gcggcgggga gaattcaacc
     3541 tgcaaggtta ggacctgcaa gattgttttt taatacaacc tgcatggttt agacctgcaa
     3601 ggttgtcctg ttctattccg gggtaaacag ttataaacgt ttgcagctac ttgctgtcgc
     3661 atcgtcagtc ctgccaggaa aaacaacaca gcaccaacct caaaaaaaac ggaacccgga
     3721 acctgaaaaa caggaatcat ttcaccacct cttcgtcgcg gaactggagc tcgaaaagct
     3781 ttttgtaatg gcctcccatg gcgagaagct gctggtgcga cccggattcg atgatctggc
     3841 catggtcgat gaccagtatt ctgtctgcgt tctggatcgt ggcgagacgg tgtgcgatga
     3901 cgatcgacgt ccttcctttc gtgagcgttt ttgtggcgcg ctggataagg atctctgatt
     3961 ccgtatcgat cgacgaggtt gcttcgtcga gcacgaggat cgtcgggtcg taaacgtagg
     4021 cacggatgaa cgcaatcagc tgccgctgtc ccacggaaag tgtgccgccg cgttcacgca
     4081 cgtcgtagtc gtaatcgccg ggaagctgca tgatgaaatc gtgcgcgccc accgctttcg
     4141 atgcggcaat gacgcgttca cgggaaattt cgggattgta cagcgtgatg ttgttgaaga
     4201 tcgtatccga aaaaagaaaa acgtcctgca gcacgatgcc catgtggctg cgcaggttct
     4261 tcaggttata ggaacggatg tctttcccgt cgatccggat cgaacctttc tggaattcat
     4321 aaaagcggtt gacgagattg atgatcgatg tttttcccga acccgtcgcg ccgacgagcg
     4381 caattgtttc tcccggcttc acgccgaaag aaattccttt gagcacccag tcttcattct
     4441 tgtacgcgaa ccagacgtct tcaaacacaa cttccccgcg tattttttcc gtgcttccgt
     4501 tctcgaccgg atcaatggcg gcttccgtgt ccatcacttt gaaaacgcgc tccgacgaaa
     4561 ccattcccat ctgcagcgta ttgatgcggt cggccagcat gcggatggga cggaagaact
     4621 ggttgatgag gatgacgaac atggcgatgt ctcccggacc atcgaagccg ctgtacacgc
     4681 gtttaccggc gaaccaaacc atgagcccga gcgaaacgga aaccaggatt tccacga
//
LOCUS       DASTSF010000245         3934 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_280146,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000245 DASTSF010000000
VERSION     DASTSF010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3934)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3934)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3934
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280146"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1003
                     /locus_tag="VFU15_07090"
     CDS             <1..1003
                     /locus_tag="VFU15_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008241689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEU4717579.1"
                     /translation="SMEITGAVVALDSTEAVVEEAIRNNCNLVIAHHPIVFSGLKKFT
                     GKNYVERTIIKAIKNDIAVYAIHTNLDNVQAGVNAMIAKKLSLKNTRILSPKENLLRK
                     LVTFCPKTEAEKVRNALFEAGAGAIGNYDECSFNAEGTGTFRGNEQTNPYVGEKGKRH
                     LENEERIETIYPQHLEGRILAALRASHPYEEIAYDIYPLLNKWQQVGSGMTGETETVF
                     SLEDFLAHVKRSLAVPVIRYTPVEGKKVKKVAVCGGSGSFLLNDAIRAGADVFITADY
                     KYHQFFDGDGKIVVMDVGHFESEQFTIQLLADFLKQKFPTFAVRQTGVNTNPITYYT"
     gene            1000..1815
                     /locus_tag="VFU15_07095"
     CDS             1000..1815
                     /locus_tag="VFU15_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018669038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HEU4717580.1"
                     /translation="MTTKAKKADDKNEVPVEEKLRAIYTLQQIDSQIDRIRTVRGELP
                     LEVRDLEDEVTGLETRLNNLNDEMKALEDSITEKKNAMKDAAAQIKKYEGQQGKVRNN
                     REFDSLSKEIEFQNLEIQLAEKRIKEFKAQIVAKKELIDASAGILEERKNDLKMKKDE
                     LEDIVSETRKEEDDLLAKSKAAEAKIEPRLLNAYKRIRTNVRNGLAVVPVERDACGGC
                     FNKIPPQRQLDIRTHKKLIVCEHCGRILVDADFLLGHKTEEKEKEKAEKSKAK"
     gene            complement(1884..3332)
                     /locus_tag="VFU15_07100"
     CDS             complement(1884..3332)
                     /locus_tag="VFU15_07100"
                     /inference="COORDINATES: protein motif:HMM:NF015316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HEU4717581.1"
                     /translation="MKRILVAGLAGLACSCALFGQNETDALRYSQTGFGGTARFSGMA
                     GAFGAVGADVSSFNINPAGLGLYHKSELVFTPSFFSQNVNSTYNGTQSLDSRYNFRFD
                     NFGVVFAGKTSNESETGWQNASMGITYNRTTTFEANLNMKGVSSTSMLDSWARSAHGT
                     GPSQLDPFNEGLAWNAWLLNTVPGDTTKYTDVIPDNDPLNQDKAINMRGGMGEWLFDV
                     AGNYSNKLYIGGAISVTTLRYEENSDYTEQEVVDSTSHFNYLKFTQLLTTQGKGFAFK
                     AGVIYRPMDMVRVGFSFQSPTILRLSDAWQSSMYSVLTDSIYNVASPEGNFNYTIRTP
                     MRATASAALIFGKSGMVSVDYEIVDYSSAIIKSTQYSFANENAAIKDKYTMANNVRIG
                     GEYRLLPLVLRAGVGYYGSPYKPSINNNAAKLYFTGGVGYRDPDDQFYVDLAAITSHE
                     ISNYYFYDQSLVNPVKNAWNSLDVVLSIGFRY"
     gene            complement(3350..>3934)
                     /locus_tag="VFU15_07105"
     CDS             complement(3350..>3934)
                     /locus_tag="VFU15_07105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717582.1"
                     /translation="DGRAPGSGGPDFGDRFIGAVASENHIVDPTQREINRVVVDQHEQ
                     NVANAQATASGNQPGDSRGTANTGNSSNPVNGRTDVRNGAGTNGSTMQRSDTPGNPYY
                     NQPNNSGRSDSHPAIQNQPQQNDNTPSRSTPTYHSQPDNNTSRPHFNSSSSSAPTFDR
                     GSSGGGGGGGRSSSGSAGGGTRSSGGGGTSSRPR"
BASE COUNT          978 a         1013 c         1083 g          860 t
ORIGIN      
        1 atcgatggaa atcacaggtg cggtcgttgc gctggacagc acggaagcgg tggtggaaga
       61 agcgatccgg aacaactgca atcttgttat cgcgcatcat cccattgttt tttccgggtt
      121 gaaaaaattc accggtaaaa attacgtgga gcgcacgatc ataaaagcga taaagaatga
      181 catcgcggtt tatgccattc acacgaatct cgataatgtg caggcgggcg tgaatgcgat
      241 gatcgcaaaa aagctttcgc tgaaaaacac gcgcatcctt tccccgaaag agaacctcct
      301 gcgcaagctc gtcacgttct gcccgaaaac ggaggcggaa aaagtgagaa acgcattgtt
      361 tgaagccggt gccggagcga tcggcaatta cgatgaatgc agcttcaatg cggaaggaac
      421 cggtacgttc cgcgggaatg aacaaacgaa cccgtacgtc ggtgaaaaag gtaaacgtca
      481 cctggaaaac gaagagcgca tagagaccat ttacccgcag cacctcgaag gacgcattct
      541 cgctgcgctg agagcatcac acccgtatga agagatcgct tatgacattt acccgctgct
      601 gaacaaatgg cagcaggtcg gttcgggaat gacgggcgaa acggaaactg ttttttccct
      661 ggaagatttc ctcgcccatg tcaaacgttc gcttgccgtt cctgtgatac gctatacgcc
      721 ggtggaagga aagaaggtga aaaaagtggc cgtatgcgga ggctcgggga gcttcctgct
      781 gaatgatgcc atccgcgccg gggccgatgt tttcatcacc gccgattaca agtaccacca
      841 gttcttcgac ggggacggaa aaatcgtggt gatggacgtg ggacactttg aaagtgagca
      901 gtttacgatc cagctactgg ctgactttct aaagcaaaaa tttcctacct ttgcagtccg
      961 ccagaccggc gtaaatacca atccgataac gtattacaca tgacaaccaa agcgaaaaaa
     1021 gcggatgaca agaacgaagt tcccgttgag gaaaagcttc gtgccattta tacgcttcag
     1081 cagatcgatt cccagattga ccggatccgt acggtgcgcg gggagcttcc cctcgaagtg
     1141 cgcgatctgg aggacgaagt aacgggcctc gaaacgcgtc tgaacaacct gaacgacgag
     1201 atgaaagcgc tcgaggattc cattacggaa aagaagaacg cgatgaagga cgctgctgcg
     1261 cagatcaaaa aatacgaagg ccagcagggg aaagtgcgaa ataaccgcga gttcgattcg
     1321 ctgagcaaag agatcgagtt ccagaacctc gagatccagc ttgccgagaa gcgcatcaag
     1381 gaattcaaag cgcagatcgt agcgaagaaa gagctgatcg acgcttctgc aggaattctc
     1441 gaagaaagaa agaacgatct caagatgaag aaggacgagc tggaggatat cgtttccgaa
     1501 acccggaaag aagaggatga cctcctggcc aaatcaaaag cagcggaagc gaagatcgaa
     1561 ccgcgcctgc tgaatgccta caagcgtatc cgtaccaatg ttcgcaacgg tcttgccgtt
     1621 gttcctgtgg aacgcgacgc gtgcggaggc tgcttcaaca agattccccc gcagcgccag
     1681 ctcgatatcc gtacgcacaa gaagctcatc gtttgcgaac actgcggacg tattctcgtc
     1741 gacgcggatt ttcttcttgg tcacaaaacc gaagaaaaag aaaaagagaa agccgagaaa
     1801 tcaaaagcga aataatcttt cgccagcaca taaaagaaaa ggaaccactg gttccttttt
     1861 ttattgctta caacgcgggt tgctcagtaa cggaacccga tcgacagcac gacgtcaagg
     1921 gaattccagg cgttcttcac cgggttcacg agcgactgat cgtagaaata gtaattgctg
     1981 atctcatggc tggtgatcgc ggcgaggtcc acataaaact ggtcgtcagg atcacggtag
     2041 ccaacaccgc cggtgaagta gagctttgcc gcgttgttat tgatcgaggg tttgtaagga
     2101 cttccgtagt aaccgacgcc ggcgcggagc acgagcggaa gaaggcggta ttctcctcca
     2161 atgcggacgt tgttggccat cgtgtattta tccttgatgg cggcattttc gttcgcgaaa
     2221 gaatactgcg tcgatttaat gatcgcagaa gaataatcaa cgatctcgta gtccacgctc
     2281 accatgcctg acttgccgaa gatcagcgcc gcgctcgcgg ttgcgcgcat aggcgtgcgg
     2341 atcgtgtagt tgaagtttcc ttccggcgaa gcaacgttgt aaatgctgtc cgtcagcacg
     2401 gaatacatcg acgactgcca tgcgtccgaa aggcggagaa tggtcggcga ctggaatgaa
     2461 aatccgacgc gcaccatgtc catgggacga taaatgacgc ctgccttgaa cgcgaagcct
     2521 ttaccctgcg tggtcagcag ctgcgtaaat ttcaggtaat tgaaatgcga cgtcgagtcg
     2581 acgacttcct gttccgtgta atcggagttc tcttcatagc gcaatgttgt cacgctgatc
     2641 gcaccgccga tgtaaagttt gttggagtaa tttcctgcca cgtcgaaaag ccattcaccc
     2701 attccaccgc gcatattgat cgctttgtcc tggttcagcg gatcattgtc gggaataaca
     2761 tcggtgtact tcgtcgtgtc gccgggaaca gtgttgagca gccatgcatt ccaggctaat
     2821 ccttcattga acgggtccag ttgtgaaggc ccggtcccgt gcgcgcttct cgcccagctg
     2881 tcgagcattg acgtcgaaga aacgcccttc atgttcaggt tggcttcgaa tgttgtagtt
     2941 cggttgtacg taattcccat cgaagcattc tgccagccgg tttctgattc gttggatgtt
     3001 tttcccgcga agaccacacc gaagttgtcg aagcggaaat tgtaccgcga atcaagcgac
     3061 tgcgttccgt tgtaggtcga atttacattc tggctgaaga aggaaggtgt gaaaacgagc
     3121 tcgcttttat gatacaagcc aaggcccgcc gggttgatgt tgaaagagct tacatctgca
     3181 cctacagcgc cgaaagcgcc tgccatcccg ctgaaccgcg ccgttccgcc gaagccggtc
     3241 tgcgaatagc gcaatgcgtc cgtttcattc tgcccgaaaa gtgcgcagct gcaggcaaga
     3301 cctgcaaggc ccgctaccag tatacgtttc atgcgataat tttttgaaat taacggggac
     3361 gggagctggt cccgcctcca cctgatgacc gtgttcctcc acccgcgctg ccggagctgc
     3421 ttcgtccgcc gccgccacct ccgccgctgc ttccgcgatc gaacgtcggg gcggagctgc
     3481 tggagctgtt gaaatgagga cggctggtat tattgtccgg ctgtgaatga tatgtcggcg
     3541 ttgagcgcga aggcgtattg tcgttctgct gcggctggtt ctgtatcgcc gggtgactgt
     3601 ccgaacggcc ggaattattc ggctggttgt aataaggatt tcccggcgtg tccgaacgct
     3661 gcatagtgga accattggtg cccgctccgt tccggacatc tgtgcgcccg tttaccgggt
     3721 tggaagaatt ccctgtattc gctgtgccgc ggctgtcacc gggctggttg ccagaagcag
     3781 tagcctgcgc gttcgccaca ttctgctcgt gctgatccac aaccacgcgg ttgatctcac
     3841 gctgcgtcgg atccacgata tggttttcac ttgccacagc gccgatgaag cggtctccga
     3901 aatcaggtcc gccgcttccc ggggcacgcc cgtc
//
LOCUS       DASTSF010000246         4502 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_15667,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000246 DASTSF010000000
VERSION     DASTSF010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4502
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15667"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1361)
                     /locus_tag="VFU15_07110"
     CDS             complement(<1..1361)
                     /locus_tag="VFU15_07110"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU4717583.1"
                     /translation="MIVFFSGSICAQPDKADSISRILGFQNDTNRVRLLLQLSAAYSA
                     YAPRKGIQPASDARILSAQIGNDRLEALSLTTLGGVYHKSGNYDDAIDNYEQSQKIYS
                     QLGLDDSVAHVSIMEGNVYIDKGNYDEALKQSFNSYSLYQKLGNKPGMASALIVSSNV
                     YRAMHNYNKAVSDLDQALSISREIGDKKNEAGCLNNLSNLYGDKGDHDKAISYLEQAK
                     EIYESLNDRFNIGKILNNIGTVYLEMSETDTSAGSSGLLDKAEDYFLQSLEIRKQVGD
                     KRGMSLTLNNLAAVSADKGNNEKAVEYLTRAIEIAKNIGALDLEVDMLEALSECYEGI
                     GDQKKALDFMKQYSAMKDSLFSENLSESVAEMQAKYGVEKAEGEALAQRQQNRIIVIF
                     SIIGAVLLLAIVFILWNRAVTRKKVNVRLNQQKQEIEQKNQELHAANEEIELKNKDIT
                     DSIT"
     gene            complement(1479..1892)
                     /locus_tag="VFU15_07115"
     CDS             complement(1479..1892)
                     /locus_tag="VFU15_07115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717584.1"
                     /translation="MAEKGRIKVIKDSSGGTLTDKGGNDLDFTQIYITELFLNEGDVV
                     RFDRATINGKPAAINVVHQTIGTITAINDDNCTGIIQEKEPNPNKLGKGVPPNIPFYT
                     PNLKELGFLVGDLVHYQLLYTKAGDIVAVNLVEAG"
     gene            complement(2003..2950)
                     /locus_tag="VFU15_07120"
     CDS             complement(2003..2950)
                     /locus_tag="VFU15_07120"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013930005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEU4717585.1"
                     /translation="MKALTETSFHFPGQTAYYKGKVRDVYTVDGLLVMIVSDRISAFD
                     VVLPRGIPYKGQVLNQVAAHFLEATKDIVPNWVIAVPDPVVTVGRKCEPYKVEMVIRG
                     YLSGHAWREYKSGKRTICGVAMPDGLRESDPFPEPIITPTTKASVGHDEDISKEEIIR
                     RGIVPAAEYGQLEEYTRALYARGQEMAGKQGLILVDTKYEFGKADGKIYLMDEIHTPD
                     SSRYFYADGYAERQEKGEAQKQLSKEFVRQWLIENGFMGKEGQSVPAMTDEFVNMVSA
                     RYIELYEKVTGKKFERVPFTEERVEKSILDFLAARKIVL"
     gene            complement(2934..3908)
                     /locus_tag="VFU15_07125"
     CDS             complement(2934..3908)
                     /locus_tag="VFU15_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013632202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEU4717586.1"
                     /translation="MAEKNIEIVSVDPIVIYGVNDAKLDYIRTFFPKLKIVARGDIIR
                     TVGDTREINRFEETIDALIQYFRRYGTLTQDNIGDILGNGNVPEGKENGNGENGNDIL
                     VYGNSGLIVKARTPGQLRMVESIAKNDMVFAIGPAGTGKTYTAVALAVRALRNKEVRK
                     IVLTRPAVEAGENLGFLPGDLKEKLDPYLQPLYDALFDMIPSDKLRQYIESNVIQIAP
                     LAFMRGRTLDNAFVILDEAQNATESQLKMFLTRMGPSAKFMITGDVTQIDLPKHQPSG
                     LLQAIRLLNNAEGIDFVYLDQRDVIRHKLVKRIIDLYDKRKKENESTD"
     gene            4039..>4502
                     /locus_tag="VFU15_07130"
     CDS             4039..>4502
                     /locus_tag="VFU15_07130"
                     /inference="COORDINATES: protein motif:HMM:NF014003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HEU4717587.1"
                     /translation="MAVITLTTDFGLRDHYTGSLRGALLRQLPSATVIDISHQITPFD
                     FDQAAYVLGHAYHEFPAGTIHLVAVGSGGGGKFPHVIVQLGEHFFIGCDNGMFSLLSD
                     KNPSAVIELAAGGKEPSTFVARDLYVPAAAALANGTPAAQLGRALEDIQRRNR"
BASE COUNT          901 a         1218 c         1194 g         1189 t
ORIGIN      
        1 gtgatggaat cggtgatgtc tttgttcttg agctcgatct cttcgttcgc ggcatgcagt
       61 tcctggttct tctgctcgat ctcctgcttc tgctggttga gacggacgtt gaccttcttc
      121 cgcgtcacgg cacggttcca caggataaac acgatcgcca gcagcagcac ggcgccgatg
      181 atcgagaaga tgacaatgat cctgttctgc tggcgctgag cgagcgcttc accttccgct
      241 ttttcaacgc cgtatttggc ctgcatttcc gcgacgcttt cgctcaggtt ctcgctgaac
      301 agcgaatcct tcatcgccga atactgcttc atgaaatcca gcgctttttt ctggtcgccg
      361 atgccttcgt agcattcgga aagcgcttcg agcatgtcca cctcgaggtc gagcgcaccg
      421 atgttcttcg cgatctcgat ggcgcgtgtg aggtattcca ccgctttttc attgttccct
      481 ttgtcggcgc tgaccgccgc gaggttgttc agcgtaagcg acatgccgcg cttgtcgccg
      541 acctgcttgc gtatttccag cgactggagg aaataatctt ccgctttgtc gagcagtcct
      601 gaagaacctg cgctcgtgtc ggtttccgac atttccaggt aaaccgtccc gatgttgttg
      661 aggatcttcc cgatgttgaa ccggtcgttg aggctttcgt aaatttcttt tgcctgctcg
      721 aggtagctga tggccttatc gtgatctcct ttatcgccgt acaggttgga caggttattg
      781 aggcagcccg cttcgttttt tttgtcgccg atctcgcggc tgatggacaa cgcctggtcg
      841 agatcactca ccgctttgtt gtaattgtgc atggcgcggt acacgttgga ggacacgatg
      901 agcgcgcttg ccattcccgg tttgttgccc agcttctggt aaagggaata ggaattaaaa
      961 ctttgcttga gcgcctcatc gtagtttccc ttatcgatgt acacattgcc ttccattatg
     1021 gaaacatgcg caacggaatc atcgagcccg agctggctgt agattttctg cgactgctcg
     1081 taattatcga tggcgtcgtc gtaattgccg gatttgtggt aaacaccgcc gagcgtggtc
     1141 agcgaaagcg cttccagccg gtcgttcccg atctgcgccg aaaggatgcg cgcgtcgctg
     1201 gctggctgga ttcccttccg cggcgcataa gcagaatacg ctgcgctgag ctgaagcagc
     1261 aggcggacac ggttcgtgtc attctggaac ccgagaatgc ggctgatgct gtcggcttta
     1321 tccggctgag cgcagatact tccgctaaaa aacacgatca cgaacagcaa aagaggaaga
     1381 cgtaatttcc gggaagaaat catacaaggc cgcaggttac cctcccaaga taaaaaaaag
     1441 cgggaaaact tcccgccttt caaaaaagct gctgccgctt aaccggcttc gacgagattg
     1501 acggcaacga tatcgcccgc cttcgtgtag agaagctggt agtgaacaag gtcaccgaca
     1561 aggaatccaa gctccttcag gttcggcgtg tagaacggaa tattcggggg aactcctttt
     1621 cccagcttgt tcgggttcgg ctctttttcc tggataatac cggtacagtt gtcgtcattg
     1681 atcgcggtga tcgttccgat ggtctggtga acgacattga tcgcagccgg cttgccgttg
     1741 atcgttgccc tgtcgaaacg cacaacgtcg ccttcattca ggaaaagttc ggtgatatat
     1801 atctgggtaa aatccagatc gttcccgcct ttgtccgtaa gggttccgcc ggaactgtct
     1861 ttaatgactt tgattctgcc tttttcagcc ataaaatttt gttttggtgt gaggattggt
     1921 tggttatcct gataaaaata gaaaatcagg attagcaggc aatagccgga tcgctttttt
     1981 tacgctgaac gatacattcc ctttacaaaa ctattttacg tgcagccagg aaatcaagta
     2041 tgcttttttc cactctttct tcggtaaaag gaacacgttc gaattttttt cccgtcactt
     2101 tctcgtaaag ctcgatgtaa cgcgcggaaa ccatattcac gaattcatcg gtcatcgcgg
     2161 gaacgctttg tccctctttt cccatgaacc cgttttcaat cagccactgc cgcacgaatt
     2221 ctttcgagag ctgtttctgc gcttcgcctt tttcctggcg ttccgcatag ccgtccgcat
     2281 aaaaataacg ggaagaatcc ggcgtgtgga tctcgtccat caggtaaatt tttccgtccg
     2341 cttttccgaa ttcgtatttc gtgtcgacga ggatcagtcc ctgctttccc gccatttcct
     2401 gtccgcgcgc gtaaagtgcg cgggtgtatt cttccagctg cccgtattcc gcggccggaa
     2461 caattccgcg gcggatgatc tcttctttcg aaatgtcctc atcgtgtccc acggaagctt
     2521 tcgtcgtcgg tgtgatgatc ggctccggga acggatcgct ttctctcagt ccgtcgggca
     2581 tggcgactcc gcagatcgtg cgttttcccg atttgtattc gcgccatgcg tgcccgctga
     2641 ggtaaccgcg gatcaccatt tcaactttgt aaggttcgca tttccgcccg acggtcacca
     2701 cgggatcggg aacggcgatg acccagttgg gaacaatgtc tttcgtcgcc tcgagaaaat
     2761 gtgcggccac ctggttcagc acttgtcctt tatacgggat gccccgcggc agcaccacgt
     2821 cgaacgccga tatcctgtcc gacacgatca tcacgagcag gccgtccacg gtgtaaacat
     2881 cacgcacttt ccctttgtaa taggcggttt gcccgggaaa atggaaactg gtttcagtca
     2941 gtgctttcat tttctttttt ccgtttatcg tacaggtcga tgatccgttt gaccagcttg
     3001 tggcggatga cgtcgcgctg gtcgaggtaa acaaagtcga tgccttccgc gttattcagc
     3061 aggcggatgg cctggagcag gccgctgggc tggtgtttcg gcaggtcgat ctgcgtaacg
     3121 tcgccggtga tcatgaattt ggcggacggg cccatgcggg taaggaacat tttcagctgg
     3181 ctttccgtgg cgttctgcgc ttcgtcgaga atgacgaacg cgttgtcgag cgtgcggccg
     3241 cgcatgaacg caagtggcgc tatctggatg acgttgcttt cgatatactg gcgcagcttg
     3301 tcggacggga tcatgtcgaa cagtgcgtca tagagcggct gcaggtacgg atccagcttt
     3361 tctttcaggt cgcccggaag gaagccgaga ttttcgccgg cctcgaccgc gggacgcgtg
     3421 aggacgatct tgcgcacttc cttgttgcgc agcgcacgca ctgccagcgc aacggcggta
     3481 tatgttttgc ccgttccggc cgggccgatc gcgaacacca tgtcgttctt cgcaatgctt
     3541 tccaccatcc ttaactgccc cggcgttctt gcctttacaa tgagcccgct gttgccgtaa
     3601 acgagaatgt cgttgccgtt ctccccgttc ccgttctctt ttccttcagg aacatttcca
     3661 ttgccgagta tgtccccgat attgtcctgc gtcagcgttc cgtaacgcct gaaatactgg
     3721 atgagcgcat cgatggtttc ctcgaagcgg ttgatctcgc gggtgtcgcc taccgtgcgg
     3781 atgatgtcgc cgcgagcgac gatcttgagt ttcggaaaga aagtgcggat gtaatccagc
     3841 ttcgcatcgt taaccccgta gatgacgatc gggtcgacgg aaactatttc aatgtttttt
     3901 tcagccaagc gtaaaagact tatttcggaa tggaacgcag cgggactaca gcaacaaatt
     3961 tgtcaggtgc actaatcctt tcgctaattt tgtcacaaaa taaacggatt tcgcccgaac
     4021 tgcaagggca aaaaaggaat ggcggtcata acgctgacaa ccgatttcgg attgcgggac
     4081 cactacacag gctctttacg tggtgcgctg cttcgtcagc ttccgtcagc aacggttatc
     4141 gatatttccc accagatcac gccttttgat ttcgaccagg ctgcatacgt gctcggtcac
     4201 gcctatcatg aatttcccgc aggcaccatt catctcgtcg ccgtcggaag cggtggcggc
     4261 ggaaaattcc cccacgtgat cgttcagctc ggcgaacatt ttttcatcgg ctgcgacaac
     4321 gggatgttct ctctcctgtc cgacaaaaat ccgtcggcgg tcattgagct ggcggccggg
     4381 ggcaaagaac cttccacgtt tgtcgcaaga gatctttacg tgccggcggc agccgcgctg
     4441 gcaaacggta cgccggcggc gcagctggga cgggcgctgg aagatataca gcgcaggaac
     4501 cg
//
LOCUS       DASTSF010000247         2391 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1885842,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000247 DASTSF010000000
VERSION     DASTSF010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2391
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1885842"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            34..363
                     /locus_tag="VFU15_07135"
     CDS             34..363
                     /locus_tag="VFU15_07135"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEU4717588.1"
                     /translation="MKTRFTLDFKIFSLILKELRAIKRELKNVKGTPAATPAPKREIK
                     DKIYSQDVLKRLRISPATLIKYEKRGLIKFHKEGRSKVYSEAEILAFRKLKRGRKRLG
                     KNFSPEQ"
     gene            1101..1673
                     /locus_tag="VFU15_07140"
     CDS             1101..1673
                     /locus_tag="VFU15_07140"
                     /inference="COORDINATES: protein motif:HMM:NF025431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4255 domain-containing protein"
                     /protein_id="HEU4717589.1"
                     /translation="MVDKALYFLRQELNSYLKLKTGDDNKVTLTAIVDQSGRTVVPDN
                     SIGMMAVNVEEERMYRSQAPQTVISNGQYAFANPELKLNIYVLFAGNHTDHREALVLL
                     SYVIQFFQAKNVFDNQESPQLGDAIEKLIVDLFSLSFEQQNQLWASLGAKYMPSVVYR
                     MRMLIINEKLATSVMPAITEIDNAFAGNRE"
     gene            1677..>2391
                     /locus_tag="VFU15_07145"
     CDS             1677..>2391
                     /locus_tag="VFU15_07145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717590.1"
                     /translation="MVTSFKSIFQVNLLHNFYTDGKCSDFVLFPLNETIDLLRNRQAV
                     FRSKDNYIRTLILGDSSGPGAAVPLEKNESCYFGMRLITPSFSNITNEYPSDKKIFLF
                     TNENNTSDPSAAAIALERKEIALCGPVINHTVVSAAGVTLKLKNVSGQVIQTKNYAAG
                     SAGTNHQFDIQRLPKGLYTVTEDDGATTIYTYYADNDLVFDSVFGIIRIVNQPDFLFT
                     YDGKPIYQVSFTSRSSLWNY"
BASE COUNT          676 a          603 c          555 g          557 t
ORIGIN      
        1 cggaacttat tcggttacac ctaactcaac gaaatgaaaa caagattcac actcgacttc
       61 aaaattttca gtcttatcct gaaagagctt cgtgcgatca agcgcgagct gaaaaacgta
      121 aaaggaacgc cggctgcaac cccggccccc aaacgtgaaa tcaaagacaa gatctacagc
      181 caggatgttc tgaaaagact ccggatatct cctgccacgc tgatcaagta tgaaaaaaga
      241 ggactgatca aatttcacaa ggaaggtcgc agtaaagtat attccgaggc ggagatactc
      301 gctttcagga agctgaaacg aggaagaaaa cggctcggga agaatttttc tcctgagcaa
      361 taaaaagaat gaggggccga aaggcccttt tttatttttc tatttctgca tgcagcaatg
      421 acgtacggcc acactaacgg cgcggaagtt ttctcccaaa cgatcatcgc tgaaaatccc
      481 cggacttcaa atccattgtt ccggcaccgg cagacccgcc ccgggatttg ggattttcca
      541 tacccgtatc tccggaatat attcttgtct taccgcaaaa caacatcttt ttatacgtgc
      601 aaacccaccg gctgcgcccg ggaagggaaa tgtgataatt aaaaaaagga aaatcaagga
      661 aataaaacat taagaaaaac gcatcaccca aataattatt tcaattctaa atcgataata
      721 ctaccggttg ttaataaccc tgttactaaa aaatgataag tgtctaataa aatgcgacga
      781 aaattaattt gccggttatt cgatttaaat accttgccgc ctcaatcact gctttcagtt
      841 cttgcttaat tgatgttatt aaaaaatcca gacgggcttt ctgcttcccc cgaaagcctg
      901 tttcccggag tgtggcctct ctatttggct ccgggttgcg ccgaccagga ttgaactgag
      961 aacaaagagc gacaaacgct cctgcggatt gaaaagggga atacgagctg tcattattaa
     1021 ggaatacggg caacccggct ttcgcatggc tattctatag ccgtgtgcgt ggtcttcgtt
     1081 gtctttttta ccagcgacct atggttgaca aagcgcttta ttttttacgg caggaactga
     1141 acagctacct gaagctgaag actggcgacg acaataaagt aactcttacg gcgatcgtcg
     1201 atcagtcggg gcgcacggtt gttccggata acagcatcgg catgatggcg gtgaacgtgg
     1261 aggaagagcg catgtaccgc tcgcaggcgc cgcagacggt gatcagcaac gggcagtatg
     1321 cgtttgccaa tcccgagctg aagctgaaca tatatgtatt gtttgccggc aaccacaccg
     1381 atcaccgcga agcgcttgtg ctgctttcgt acgtcatcca gtttttccag gcaaagaatg
     1441 tttttgataa ccaggaatcg ccgcagctcg gcgacgcgat cgaaaagctg atcgtcgacc
     1501 tgttctcgct gagcttcgaa cagcagaacc agctgtgggc ttcgctcggc gccaaataca
     1561 tgccgtcggt ggtataccgc atgcgcatgc tgatcatcaa tgaaaagctg gcgacgtcgg
     1621 tgatgccggc cattaccgag attgacaacg ccttcgccgg caacagagaa taaaaaatgg
     1681 taacgagctt caaaagcata ttccaggtca acctgctgca caatttctac accgacggaa
     1741 agtgcagcga cttcgtgctt tttccgctga atgaaacgat cgacctgctg cgcaaccgcc
     1801 aggctgtgtt ccgcagcaag gacaattata tccgcacgct gatcctcggc gattcttccg
     1861 gtcccggcgc agcggtgccg cttgaaaaaa acgagagctg ctatttcggg atgcggctga
     1921 tcactccatc tttttcaaac atcacgaacg aatatccttc cgataaaaag atattcctgt
     1981 tcacgaacga gaacaacacc tccgatcctt cggctgcggc catcgcactg gaacgaaaag
     2041 agatcgcgct gtgcggacct gtgatcaatc acacggttgt ttccgctgcg ggcgtaacgc
     2101 tgaagctgaa aaatgtttca ggacaggtca tccagacaaa aaattacgcc gcaggatccg
     2161 caggcaccaa ccaccagttc gacattcagc gcctgccgaa aggactttat accgtgacgg
     2221 aagacgatgg cgccacaaca atatatacgt attacgccga caacgatctc gtcttcgaca
     2281 gcgtgttcgg aattatccgc atcgtcaatc aaccagattt tcttttcacg tatgatggaa
     2341 aaccgattta ccaggtcagc tttacgtcca ggagcagcct gtggaactat t
//
LOCUS       DASTSF010000248         9514 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_945797,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000248 DASTSF010000000
VERSION     DASTSF010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 9514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9514
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_945797"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..551)
                     /locus_tag="VFU15_07150"
     CDS             complement(<1..551)
                     /locus_tag="VFU15_07150"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU4717591.1"
                     /translation="MNPSSPPAPKLGTVTAVTITTPDLESSLAFYRRLGFSELFRADF
                     PFPWIQITDGALLLMLRKDNDPYIALTYYAKDAEKIAAELEAGGITFEQKAKPSDFIK
                     RFLFRSPDGLNISIVTLADGFHQPAGPTLLNMPPADYFSPEKYPNKSCGLFGEFAHPV
                     KDIEASIAFWQKLGFRALSRHESP"
     gene            complement(581..1108)
                     /locus_tag="VFU15_07155"
     CDS             complement(581..1108)
                     /locus_tag="VFU15_07155"
                     /inference="COORDINATES: protein motif:HMM:NF025227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HEU4717592.1"
                     /translation="MKKIVLTYSLIAGAIMFAMMIIPTVFFSQNQLDSGKAEIIGYTT
                     MIAAFLLIFFGVKNYRDNYSSGTITFWKAFLTGLYIMLIASVIYVIAWMIIQPLFMPD
                     FYQKYFDLEAATLKANGATVAQLAEAKQNADSMIRLVSNPVFKFLLTLLEPLPVGLPI
                     TVISALVLKKKPAQA"
     gene            complement(1226..1729)
                     /locus_tag="VFU15_07160"
     CDS             complement(1226..1729)
                     /locus_tag="VFU15_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020213700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU4717593.1"
                     /translation="MNLSRPLFLRRFALYGIALALITVLLKWMEYRFLIISHAWEIYA
                     GLIAVSFTVIGAWAGWKITRKKPEKEIVIEKEIVVVEKEVIVEKEVPAPFSPDPQAAE
                     KLGISQREMEVLHLMAEGLSNQEIAGKLFVSLNTVKTHLSNLFQKLDVSRRTQAVQKA
                     RTLRLVK"
     gene            complement(1734..2255)
                     /locus_tag="VFU15_07165"
     CDS             complement(1734..2255)
                     /locus_tag="VFU15_07165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717594.1"
                     /translation="MASVRAKLIPALLFISFFIFSCETPTHKIDLGDNAKEHAQTKNS
                     SVTVSRTPIGNTGYSIELPSSHMIQAQPGADFQLFYFTPHDTVLHRGEAGMYIGAHPD
                     VNPPKGDVSSKEMTGILLGKQVTWIEYEMEEYTQRETFVDMGNDQYIHVWCYANNLPE
                     LENLFKMVNTITY"
     gene            complement(2383..2772)
                     /locus_tag="VFU15_07170"
     CDS             complement(2383..2772)
                     /locus_tag="VFU15_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007657722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEU4717595.1"
                     /translation="MKPESRLHLASLILRVTSALMLTHGWKKLSTFGDKMHTFADPLH
                     IGSTASLALTVFAEFFCTLFIVTGLFTRIAVIPLIITFLVIVLIVHAPDPFGEKEPAL
                     LYLLIYTCILITGSGKYSLDHLLRKKY"
     gene            complement(2820..3155)
                     /locus_tag="VFU15_07175"
     CDS             complement(2820..3155)
                     /locus_tag="VFU15_07175"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEU4717596.1"
                     /translation="MTNEEIMSAVSKGKAYTLMFLYKGKNRAQDKTEEDRLQMEHLAY
                     LFKLREEGKLLLNGPLTGEGDLRGIAIFSLTDTEEVRRLSEEDPAVKAGRLRVEVFPW
                     FGLPGDHLR"
     gene            complement(3192..4418)
                     /locus_tag="VFU15_07180"
     CDS             complement(3192..4418)
                     /locus_tag="VFU15_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010599160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEU4717597.1"
                     /translation="MDNLILILLSSLIIFSYVFDLFARRTKIPSVILLIGLGIGLQYL
                     AAYFHFETFNFLKILPTLGTIGLILIVLEGALDLQYDKGKRRLIANAFFTALVILVLT
                     SLGITYVFRFFTGNTFQLCFVNAIPFTVISSAIAIPSVATILRKKREFIVYESSFSDI
                     FGIMLFNFAVTSDGFSGSSFLRLGTDTFLILVISAAFCIGLLFILGKISHHVKFFLII
                     SILILIYAVAHEAELPALIIILAFGLFLGNAGKITNTYFRKHLLYPAFARDIKLLHQL
                     SAESAFLLRTLFFVIFGFTMQVSDIADSNVLFYGGIILGLTYIVRGLFLKFVSRTGLI
                     PELFITPRGLINILLFYSIPAEMKIPGVESSVLFLVIIVTTSLMAVGLVRAKSDVPPP
                     EAAPAGDTVAENHPPE"
     gene            complement(4745..5971)
                     /locus_tag="VFU15_07185"
     CDS             complement(4745..5971)
                     /locus_tag="VFU15_07185"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009578463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEU4717598.1"
                     /translation="MKSFNIPEFYRSPVISKIKALRKAGDPKKKDFSPTVLDFGPAVI
                     YIARHFGFCYGVENAVEISFRSVEENPGKRIFLLSQMIHNPEVNNDLTSRGVRFIMDT
                     NGKQLIPWSELTKDDIVIIPAFGTTLAIESHLHAIGIQPERYNTTCPFVERVWKKSAQ
                     LGEGKFTIIIHGKPAHEETRATFSHSAVNGPSIVVKDMSESVLLGNFILGKIPREEFE
                     KTFSGRTSPGFDPQKDLDRIGVINQTTMLAAETQAIADHFKKVMEEKFGALNSKDHFA
                     DTRDTLCYATNENQDATYGLLETNADLAVVVGGYNSSNTSHLVELCEQRFPTYFIKDE
                     TEIISGTVIRHFDLHTHEMKQTQSWLPGRTPVRIVLSSGASCPDSVVERVLNRLLSFF
                     ENARKAEAVLAELEAK"
     gene            6309..7199
                     /locus_tag="VFU15_07190"
     CDS             6309..7199
                     /locus_tag="VFU15_07190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717599.1"
                     /translation="MRQGKKNESPRKIISPIDTLLLFRKFLGKISRYEVALVQLHGVY
                     WRMNVAGVKRLGNICKNKTMTLRPDSPLNFLPKEIGHKQLMLFDSLRFTLEMLDYNYS
                     QLIKNLEDLSCGKENKIHYKIFNYAWGLIDHGQRFYLLYKKLNPPENIIIEKINYIYS
                     FRNAIQHLDKNVECQIIANFRPIYGTLKWIVNDTDKKEIYTSLLVSGIFEIDKLEFEQ
                     HGQAGYHKFINEVKLETDTLGKKNENEINLCKMIEDISFVTDKLDAELSKRFRDRNFQ
                     LLDWKSRKDVLLNMKNPNVA"
     gene            7305..7739
                     /locus_tag="VFU15_07195"
     CDS             7305..7739
                     /locus_tag="VFU15_07195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717600.1"
                     /translation="MDKTILDNILEKHKAQPVGHGYIDIIVSRDNYKNFISDLVTTGY
                     KIDCVSWWEWCEDKKENEYGLGGPVSVFYKGWFSEIPVKVDDFNFSSDATKENIIQEV
                     LNKIETKTISFSDEIVVFKESNWLTPAIWLDVPDDWRNKYSA"
     gene            7820..8275
                     /locus_tag="VFU15_07200"
     CDS             7820..8275
                     /locus_tag="VFU15_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HEU4717601.1"
                     /translation="MKERLIILLQGVIALLGIVTLVIMIRFPLTEGRASNLNLVQIYF
                     DPLIVYGYLASIPFFIALYSIFQLLRSIRQNKGLSPKTVRIVRRIKYCAIALSILIVI
                     AALYIKLNHNKEDDPAGFLAICMLSSFGSIAVAVLSVLFEKKLQKMEPH"
     gene            complement(8374..9402)
                     /gene="mltG"
                     /locus_tag="VFU15_07205"
     CDS             complement(8374..9402)
                     /gene="mltG"
                     /locus_tag="VFU15_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008512919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEU4717602.1"
                     /translation="MKKIFLIAGLVLLVLAGAGGYWFYRIYFEPNVHTPDHKPEFIYI
                     HTGSDMQDLMLQLQENKIVDDTSSFAEIARLKKFSAPKPGRYRIRDGMSNRDLVNLLR
                     SGEQEPVEVTFNNIRTKEQLAGRVGGKLEADSAHFLLLLNDPGFTAKFGLTTDNIMTM
                     FIPNTYQFFWNTNEEQFIDRMAQEYKKFWTDDRKAKARAAGLSQSESIILASIVESEQ
                     SRFADELPVIAGLYINRLHKGIALQSDPTIIYALGDFSIQRVTNADLDINSPYNTYLH
                     TGLPPGPIRIPESATVDAVLNYNHNNYLYMCAEFGTGRHRFTDDYNQHLKNAREYQAA
                     LNKANIRR"
BASE COUNT         2369 a         2079 c         2665 g         2401 t
ORIGIN      
        1 ggggattcat ggcgggagag cgcgcggaaa ccgagtttct gccagaaagc gatggacgct
       61 tcgatgtctt tgacaggatg cgcgaattca ccaaaaagtc cacagctttt gttgggatat
      121 ttttccgggc tgaaatagtc ggccggcggc atgttcagca gggtagggcc ggccggctgg
      181 tgaaagccat cggcgagcgt gacgatcgat atgttgagcc cgtcgggtga gcggaacagg
      241 aaacgtttga tgaaatcgga cggcttcgct ttttgttcga acgtgatccc gcctgcttcc
      301 agttccgccg caatcttttc tgcgtctttt gcataatacg tgagcgcgat atacggatcg
      361 ttatccttgc gcagcatcag gagcagcgct ccgtcagtga tctgtatcca cgggaaggga
      421 aaatcggcgc ggaacaattc ggaaaacccg agcctgcggt aaaaggcaag tgatgattcg
      481 aggtccggcg tggtgatagt aaccgcggtt acggttccga gcttcggtgc gggaggagaa
      541 gatggattca taggagtgtg gagtgggatg ataaacgggt ttacgcctgc gccggttttt
      601 tcttcagcac cagcgccgag atcaccgtga tgggaagccc gaccgggagc ggttcaagga
      661 gcgtaagcag gaacttgaag acagggttcg aaaccaggcg gatcatgctg tcggcgttct
      721 gttttgcttc ggcgagttgt gcaaccgttg cgccgttcgc tttcagggtg gctgcttcca
      781 ggtcgaagta tttctggtag aaatcgggca tgaaaagggg ctggatgatc atccaggcga
      841 tgacgtagat gaccgatgcg ataagcatga tgtaaagtcc tgtaaggaac gctttccaga
      901 atgtaatggt gccgctgctg tagttgtcgc ggtaattctt cacgccgaaa aagatgagaa
      961 ggaacgcagc gatcatggtg gtatagccga tgatctcggc cttgccggaa tcgagctggt
     1021 tctgcgaaaa gaagacggtc ggaatgatca tcatggcgaa catgatggcg ccggcgataa
     1081 gcgaataggt aaggacgatc tttttcatgg ttttttgttt ttggtggcgg caaaggaaca
     1141 tgaaccggcg gggaaaagca tcacccgaaa gtatgaaatg cccgtttctc ccgctttttc
     1201 acccggaagt acgatgagcg gaaattcact ttaccagcct gagcgtccgc gctttctgca
     1261 cagcctgggt acgccggctt acgtcgagtt tctggaaaag attggacaaa tgcgttttca
     1321 cggtattcag ggaaacgaag agcttgccgg cgatctcctg gttgctcagg ccttcggcca
     1381 tgaggtggag cacctccatt tcccgctggc tgatgccgag tttttcagcc gcctgcgggt
     1441 cgggactgaa cggagcggga acttcttttt caacgatcac ttccttttcc accacgacga
     1501 tctctttctc gatcacgatc tctttttccg gttttttccg cgtgatcttc caccccgccc
     1561 acgcaccgat gaccgtgaag gaaacggcga tgagtcccgc gtaaatttcc cacgcgtggc
     1621 tgatgatgag gaaacggtat tccatccatt tcagcagcac ggtgatcagc gcgagggcga
     1681 tgccgtacaa ggcaaaccgg cggaggaaaa gcgggcgcga caggttcatg ccgtcagtag
     1741 gtgatcgtgt tcaccatttt gaaaagattt tcgagctcgg gcaggttatt ggcgtagcac
     1801 cagacgtgga tatactggtc gttgcccata tcgacgaacg tttcccgctg ggtgtattcc
     1861 tccatttcat attcgatcca cgtaacctgt ttaccgagga ggatgcccgt catttccttg
     1921 gaggaaacgt cgcctttggg cgggttgacg tcgggatgcg cgccgatgta catgccggct
     1981 tcaccgcgat gcaggaccgt atcgtgcggc gtgaaataga agagctggaa atcggcgccg
     2041 ggctgcgcct gtatcatgtg cgaggatggc agctctatgc tgtagccggt gttcccgatt
     2101 ggagtgcggc tgacggttac gctgctgttt ttcgtttgcg catgctcttt cgcgttgtcg
     2161 ccgagatcga tcttgtgcgt aggtgtttca caggaaaaaa tgaaaaagga aatgaaaagc
     2221 aacgcgggaa tgagcttagc cctgactgaa gccaactgcc gtgcagtgcg gaaggcagga
     2281 agagaggtgc tttgatgcgg ggaaggtgcg ctgcgtaaca ttaaaaaaag ttggaaagtc
     2341 gaaaagttgc cgggatgttc ttttcaaaat taaacctttc cgtcaatatt ttttccgcag
     2401 gaggtgatcg agcgaatact tgccggagcc ggtgatgaga atgcaggtat aaataagcag
     2461 gtagaggagc gcgggttctt tctcgccgaa aggatcgggt gcatgcacga tgagcacgat
     2521 gacgaggaac gtgatgatga gcgggatgac ggcgatgcgc gtgaacaggc ccgtcacgat
     2581 aaaaagcgtg cagaaaaatt ccgcgaaaac ggtcagtgcg agcgaagctg tgctgccgat
     2641 gtgcagcgga tcggcgaagg tgtgcatctt gtcgccgaag gtggacagtt ttttccagcc
     2701 gtgtgtgagc ataagcgcag aggtgacacg caggatcagt gaggcgaggt gcaggcggga
     2761 ttcgggtttc atgttttgga tgggttggga ggttgaaagg ttgggaggtc ggggagtttt
     2821 taccggagat gatcgccggg aaggccgaac cagggaaaga cttcgacgcg caatcttccg
     2881 gctttgacgg cgggatcttc ttcggaaagg cgccgtactt cttccgtgtc ggtgagactg
     2941 aagatcgcga tgccgcgcag gtcgccttca ccggtgagcg gaccgttgag cagcagcttg
     3001 ccttcttccc gcaatttgaa aagataggcg aggtgttcca tctgcaggcg gtcttcctcc
     3061 gttttgtctt gcgcgcggtt tttccctttg taaaggaaca tcagcgtata ggcttttcct
     3121 ttggaaacag cggacatgat ctcttcgttc gtcatagctg gagattaaaa aatgcgaccg
     3181 gaaattactt tttattccgg gggatgattt tccgcgacag tgtcaccggc cggggctgct
     3241 tcgggaggag gtacgtcgct tttcgcacgg acgagtccca cggccataag cgatgtagtg
     3301 acaatgataa cgaggaagag cacgctgctt tccacacccg gaattttcat ctcggcggga
     3361 atgctgtaaa aaagcaggat gttgatgagt ccccgcggcg tgatgaaaag ttccggaata
     3421 agcccggtcc ttgaaacgaa tttcaggaaa agcccgcgaa cgatgtacgt caggccgaga
     3481 atgatgccgc cgtagaaaag cacgttgctg tcggcgatgt cactcacctg catggtaaag
     3541 ccgaaaatga caaagaaaag cgtgcggagc aggaatgcgc tttccgccga aagctggtga
     3601 agcagcttta tgtcccgcgc gaaagccggg tagagcaggt gtttccggaa gtaggtgttg
     3661 gtgatctttc cggcgttgcc gaggaaaagt ccgaacgcga gaatgatgat aagcgcggga
     3721 agttcggcct catgcgccac ggcgtaaata agaatgagga tggaaatgat caggaagaat
     3781 tttacgtgat ggctgatctt cccgaggatg aaaagcagcc cgatgcagaa tgcggcggaa
     3841 ataacgagga tgaggaacgt gtcggtgccg agacgaagga aagaactgcc cgaaaatccg
     3901 tcgctggtga ccgcgaaatt gaaaagcatg atgccgaaaa tgtctgaaaa ggaagattcg
     3961 tacacaatga attctctttt cttgcggagg atcgtcgcca cggaaggaat ggcgatggcg
     4021 gagctgatga cggtgaacgg gatggcgttc acaaagcaca actggaatgt attcccggtg
     4081 aaaaaacgga acacgtacgt gatcccgagc gaggtgagca cgaggatgac gagcgcggtg
     4141 aagaatgcat tggcgatgag gcggcgcttg cctttgtcgt actgcaggtc gagcgcgcct
     4201 tcgagcacaa tgagtataag cccgatcgtg ccgagcgtcg gaagtatttt gaggaaattg
     4261 aatgtttcga aatggaaata ggctgcgagg tattgcaggc cgatgccgag accgatcagc
     4321 aggatgacgg acgggatctt cgtgcgcctc gcgaaaagat cgaagacata ggagaagatg
     4381 atgaggctgc tgagcagtat aagaatcagg ttgtccacaa cacaggaaag atatttattt
     4441 cgggcggggg aaatacagtc ttaagaaata ataataatcg gatttcgttt cgggttgtct
     4501 ttgttttcct ttgtgcgctt cgtgaaaaac cttcgtgttc ctggtggtct tgactttgca
     4561 actttgaacc tgaatgatgt tcgttttctt tgtgcgcttt gtgaaaaacc ttcgtgttct
     4621 tcgtggtttt aacattccaa ctttgaaccc ggattgtctt cgtattcctt cgtgcgctgt
     4681 gcggttaaat aaatagtttt cccgaaaaat tgccggcaga tgcctgaagc gagcttcggg
     4741 aaaattattt tgcttccagc tccgcgagca cagcttccgc tttccttgca ttctcaaaga
     4801 acgaaagaag gcggttgagc acgcgttcca ccacgctgtc cgggcagctg gcgccgcttg
     4861 aaagcacgat gcggaccggt gttcttcccg gcagccagct ttgcgtttgt ttcatttcat
     4921 gtgtgtgcag gtcgaaatgc cggatgaccg ttccggaaat aatttccgtt tcatccttga
     4981 tgaaataagt ggggaaacgc tgttcacata actcgaccag gtgcgaagtg ttggaactgt
     5041 tgtatcctcc gacaacgacg gcgagatcgg cgttcgtttc gagcagtccg tacgtcgcgt
     5101 cctggttttc attcgtggcg tagcaaagcg tgtcgcgcgt gtcagcgaaa tggtctttgc
     5161 tgttcagcgc gccgaatttt tcttccatca cttttttgaa atgatcggcg atggcctgcg
     5221 tttccgcggc gagcatggtc gtctggttga tgacaccgat gcgatcgagg tctttctgcg
     5281 gatcgaatcc cgggctcgtc cgcccggaaa atgttttttc aaattcttcc cgcggaattt
     5341 tcccgaggat gaaattcccg agcagcacgg attcgctcat gtccttcacc acgatggaag
     5401 gtccgttcac ggcgctgtgc gagaacgtgg cgcgtgtttc ttcatgtgca ggcttgccgt
     5461 ggatgatgat cgtgaatttt ccttcgccca gctgcgccga ttttttccag acgcgttcga
     5521 cgaacgggca ggtggtgttg tagcgttccg gctgtatccc gatggcgtgc aggtggcttt
     5581 cgatcgcgag cgtcgtgccg aacgcgggaa tgatgacgat gtcgtctttc gtcagctcgc
     5641 tccagggaat gagctgtttc ccgttcgtgt ccatgatgaa gcgtacgccg cgtgaggtga
     5701 ggtcgttgtt cacttccggg ttgtggatca tctggctcag cagaaaaata cgcttgccgg
     5761 gattttcttc caccgaacgg aaggagatct ccaccgcatt ttccacgccg tagcagaagc
     5821 cgaaatgtcg cgcgatgtaa atcaccgccg ggccgaaatc gagcaccgtg ggtgaaaagt
     5881 ctttcttctt cggatcgccc gccttgcgca gcgccttgat cttcgaaatg accgggctgc
     5941 ggtagaattc agggatgttg aaggatttca cggtacaaaa ataggggtta gtcttcagtt
     6001 ttgggtttga acccggaatc cggcagtttc cctgtttgcc ttttttctct gcttagtttt
     6061 tagctcgctc caaaaagtaa aagcgaaaaa agaaattccg gcccgcgtca gcccggcaga
     6121 acgattccgg cggggggaat gtgcgccaag gcgccttttt tctttcagaa gcgatggaac
     6181 gtgcgtaagt gagtgagcga agacgaacga acggggaaag aaaaaccagc gccggcaaca
     6241 aggaatcgtt cgtagaatgg gtcagtgcga caaagggcct cagcgggcct tgactttttt
     6301 ggttcttttt gcgtcaaggc aaaaagaacg aaagcccccg gaagatcatt tctcccattg
     6361 atacgctttt gctttttcgt aaatttctgg gaaaaataag ccgctacgaa gtagcgcttg
     6421 tacagctcca tggggtgtat tggcgaatga atgtagcagg tgtaaagagg ttaggcaaca
     6481 tttgtaaaaa taaaaccatg acattaagac cagactctcc attaaatttt ctgcccaaag
     6541 aaattgggca caaacaatta atgctttttg actctttgag atttacctta gaaatgttag
     6601 actacaatta ttcgcaatta atcaagaatc ttgaggactt atcttgcgga aaggaaaaca
     6661 aaattcatta taaaatattc aattacgctt ggggcctgat agatcatggg caaagattct
     6721 acttacttta caagaaactc aatcctcctg aaaacatcat aattgaaaaa attaattaca
     6781 tatattcatt tcgaaatgca attcagcact tggataaaaa tgtggaatgc caaataattg
     6841 caaatttccg accaatatat ggaacattaa agtggatagt taacgataca gataaaaagg
     6901 aaatttatac ttctctttta gtcagcggaa tattcgagat tgacaaatta gaatttgagc
     6961 aacatggaca agcaggttat cataaattta ttaatgaagt caagcttgaa acagacacat
     7021 taggaaaaaa gaacgaaaat gaaattaatc tttgcaaaat gattgaagat atatcttttg
     7081 taactgataa gttagatgca gaattaagta aacgattcag agacagaaat tttcaattac
     7141 tcgactggaa aagtagaaag gacgtattac ttaacatgaa aaatccaaac gttgcctgac
     7201 acgggttttg cgttagtggg cgtatcctgc caaggcgaga cgaatagaaa tccgctttcg
     7261 cgaagcccga aaccgttagg cgtaatatta caaacgacaa ataaatggac aagacaattc
     7321 ttgacaacat acttgaaaaa cataaagcgc aaccagttgg acacggttac attgacatca
     7381 ttgtttcccg agacaactac aaaaatttca tttccgacct tgtgacaact ggctataaaa
     7441 ttgattgcgt aagttggtgg gaatggtgtg aggacaagaa agaaaatgaa tacggacttg
     7501 gaggacctgt aagtgttttt tataaaggtt ggttttcaga aatcccagtg aaagttgacg
     7561 actttaattt ttcatcagac gcaactaaag aaaatataat ccaggaagtt ctcaataaaa
     7621 ttgaaacaaa gacaatttca ttttcagacg aaatagtagt gttcaaagaa agtaattggt
     7681 tgacaccagc aatttggtta gacgtccctg acgactggag aaataaatac agcgcctaac
     7741 acggattttt cgttagcggg cggactgtgc gaatagaaat ccgccgcggc ggaatcaatt
     7801 aaaaaagtaa aactcattca tgaaagaaag gctgataata ttactccagg gtgtcatcgc
     7861 acttttgggt attgtgacat tggtcatcat gatcaggttt cccttaacgg aggggagagc
     7921 ctcaaactta aatctggtac agatttactt tgacccgtta atagtatatg gatatttggc
     7981 atccatccct ttttttattg cgctttacag cattttccag ttactgagat ctatccgaca
     8041 aaataaagga ctttcgccaa agacggtaag aattgttcgg aggataaagt attgcgcaat
     8101 tgcgctgagc atattgattg tgatagcagc attatacatt aagttaaatc ataataaaga
     8161 ggatgatcct gccggttttc ttgcaatctg tatgctatcc tcttttggtt ctatcgctgt
     8221 agctgtatta tccgttttat ttgaaaagaa attgcaaaaa atggaaccgc attaaagcgg
     8281 gattctcatt cgtttttttt gcaggtttaa attcccggac taaaagaatt tcctgctgac
     8341 gattcagtat tcaaaaccgg aatccggaat tatttaccgg cgtatgttcg ctttattcag
     8401 cgccgcctgg tattcccgcg cgtttttgag atgctggttg tagtcgtccg taaacctgtg
     8461 acggcctgtt ccgaactccg cgcacatgta gaggtaattg ttgtggttgt aattcagcac
     8521 cgcatccacg gtggctgatt ccgggatgcg gatagggccg gggggaagtc ccgtgtgcag
     8581 gtacgtgttg tatggtgaat tgatgtcgag gtcggcgttc gtcacgcgct ggatggaaaa
     8641 atcgccgagc gcgtaaatga tcgtgggatc gctttgcagc gcaattcctt tgtgcaggcg
     8701 gttgatgtac aatcccgcga taacgggaag ttcgtcggcg aatcgcgatt gctccgattc
     8761 gacgatggac gcaaggatga tgctttccga ttgggaaagt cctgctgcgc gcgccttcgc
     8821 tttgcggtcg tccgtccaga attttttata ctcctgcgcc atacggtcga tgaactgctc
     8881 ttcgttcgtg ttccagaaga actggtaagt gttcgggatg aacatcgtca tgatgttgtc
     8941 ggtggtgagc ccgaatttcg ccgtgaagcc gggatcattg agcagcagga ggaaatgcgc
     9001 cgaatccgct tcgagctttc cgcccacgcg cccggcgagc tgctctttcg tgcggatgtt
     9061 gttgaacgta acttccaccg gctcctgctc gcccgaacgc agcaggttga cgaggtcgcg
     9121 gttgctcatg ccgtcgcgga tgcggtagcg cccgggtttc ggggcggaga attttttcag
     9181 ccgcgcaatc tccgcaaagg aagaggtgtc gtccacgatc ttgttttcct gcagctgaag
     9241 catgaggtcc tgcatgtccg agccggtgtg tatgtaaatg aattccggct tgtgatcggg
     9301 cgtatgcacg ttcggctcga aataaatgcg gtagaaccag tagccgccgg cgccggccag
     9361 cacaagcagg acaaggcctg cgatcaggaa gatctttttc acgggattat cttatcatcg
     9421 gctgaagctg cgatttcagc tgcatggcct gcgcgttctc tggattgtgt gtaagacagc
     9481 ggtcgacgta cgtcatggcg tccctgtaat cttt
//
LOCUS       DASTSF010000249         8074 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1659898,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000249 DASTSF010000000
VERSION     DASTSF010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 8074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8074
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1659898"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..640
                     /locus_tag="VFU15_07210"
     CDS             <1..640
                     /locus_tag="VFU15_07210"
                     /inference="COORDINATES: protein motif:HMM:NF027445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4835 family protein"
                     /protein_id="HEU4717603.1"
                     /translation="LQIQSVRPIYKSSYSSTMINHNDPDIEFNYVQFQPLEFSISQHL
                     NNLTSILAYYAYVILAQDYDSYSSDGGTQYWQKAQTIVNNAQNAPEKGWKSMESNTQN
                     RYYLVENMLQPIYEPVRQCNYKYHRLGFDIMYNDVNGGRAICLEALNSLEAVHEQRPL
                     SFPMQFWFNAKSQEIINLFSGGLPEEKTKVVALCQKIDPGHGLLYQKITQTQ"
     gene            718..1782
                     /locus_tag="VFU15_07215"
     CDS             718..1782
                     /locus_tag="VFU15_07215"
                     /inference="COORDINATES: protein motif:HMM:NF040069.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6340 family protein"
                     /protein_id="HEU4717604.1"
                     /translation="MKPKILRPFIFIFVVLLAGEACKMSSVRLQVLQPADIMLPQQIQ
                     KFVLADRTRPAKGGGQQALNIAEGILTGEGIFQDKWGAEDCLEGLRQVLTMTPRYTVT
                     IASLDTALKGTGRRQTMPPLDWTTVGKLVGNDTTTALVVLEAFDSNTNLFNEVVNTTQ
                     RGPDGGNVTVPEYHAHGKVDVYCVWRIYDLRSKTIVDEFTQESWQQFDGSGPTLQVAQ
                     SRLPNRRDMVSRAGVSAGIQYGHRISPQWIWVTREYYRRGSPKLKQASKLVHYNNWKT
                     ATDIWNAEASNTDPKIAKRASFNMALAAEQAGQLDLALQWADRAVQMGDKRAPRYVQI
                     LQQRQYEQQKLQEQMKGKQQ"
     gene            1872..2114
                     /gene="rpmB"
                     /locus_tag="VFU15_07220"
     CDS             1872..2114
                     /gene="rpmB"
                     /locus_tag="VFU15_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014020701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEU4717605.1"
                     /translation="MSKVCQITGKHTIVGNNVSHSNRKTKRTFQPNLHSKKFYLPEEK
                     RWITLKVSAAGLRTISKIGIGEALKRAKAKGLVKLK"
     gene            2207..3628
                     /locus_tag="VFU15_07225"
     CDS             2207..3628
                     /locus_tag="VFU15_07225"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P N-terminal domain-containing
                     protein"
                     /protein_id="HEU4717606.1"
                     /translation="MTKRFLLLPASFFLALIFFSFTGAPDHYDYDTDLLTPQFHQGRP
                     QALRDLLPDSSVAVFFASPVRTRSNDVEYDYHQDPNLFYLTGLCEPNAMLIVFKEKQQ
                     VDTMFTNELLFVQDRDPKEEAWTGKRLGADGAKQLLGIDKVLSSKDFSPSMIKWKKFH
                     TVFFFPLTGEMNDHPDDPCDLSLLLAKFSNSTKRNSNDDTERLSEYMAMLREVKQPEE
                     LELMRKAILISGDGHTELMKALEPDMTEYQAQAILEFRFKMEGAEFPAYPSICGSGEN
                     SCILHYTSNRRPFQDGDLIVVDAGAEYHGYCADITRTLPVNGKFSAEQKQIYDIVYDA
                     QQAGIQACRIGNEFRAPHNAAVEVIKKRLLALGIIQNEEDYIRYFFHGTSHYLGLDVH
                     DAGLNGRLALGNVITVEPGIYIPAGSPCDPKWWNIGVRIEDDVLITSGDPEVLSGNIP
                     KTTTDIEAMMAQKSYLNESGGPR"
     gene            3762..3944
                     /gene="rpmG"
                     /locus_tag="VFU15_07230"
     CDS             3762..3944
                     /gene="rpmG"
                     /locus_tag="VFU15_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEU4717607.1"
                     /translation="MAKKGNRIQVILECTEHKESGKPGTSRYITTKNKKNTPDRIELK
                     KYNPILKKVTVHKEIK"
     gene            3968..4126
                     /locus_tag="VFU15_07235"
     CDS             3968..4126
                     /locus_tag="VFU15_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009596369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4295 domain-containing protein"
                     /protein_id="HEU4717608.1"
                     /translation="MAKKVVATLKTGEGKQFTRVIKMVKSDKTGAYSFREEVVHNDHI
                     KDFLSEKK"
     gene            4199..5155
                     /gene="ftsY"
                     /locus_tag="VFU15_07240"
     CDS             4199..5155
                     /gene="ftsY"
                     /locus_tag="VFU15_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004295981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEU4717609.1"
                     /translation="MGIFGFFSKEKKESLDKGLSKTKESFLSRIARAVVGKSTVDSEV
                     LDNLEEILVTSDVGVDTTLKIIKRIEDRVSKDKYVNTSELDKILRDEIAALLAENKSE
                     DLQDFSVPEGKKPYVIMVVGVNGVGKTTTIGKLANAFKKSGKSVMLGAADTFRAAAVD
                     QLTIWSQRVDVPIVAQGMGADPASVAFDTLNSAVAKGSDVVIIDTAGRLHNKVNLMNE
                     LTKIKKVMQKVVPDAPHEVLLVLDGSTGQNAIEQCKQFTAATDVTALAVTKLDGTAKG
                     GVVIGISDQFKIPVKYIGVGEKIDDLQVFNKTEFVDSLFRKD"
     gene            5387..7450
                     /locus_tag="VFU15_07245"
     CDS             5387..7450
                     /locus_tag="VFU15_07245"
                     /inference="COORDINATES: protein motif:HMM:NF019315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HEU4717610.1"
                     /translation="MLRKSILVFALLLAPFFLAAQDVVVLKDNAPVTSLVGHYEVFVD
                     STGNMSLGEVMHSGKFYKPLQPNPNFGITSSAIWARFTLKKTGKARQLFSYGNCGSDS
                     VILYETGGPTTGKQIMSGRDSYRKREFGVNLALFSLAIPADSTRTFYLRVKDVPPVMM
                     VLEAGTEESYFTYASLYNLLNGVFYGIILLMIIYNLFLFFTNRDPVYIYYVVYTLANG
                     LFIAFVNGYLIYFPHWLNSLFFRLHTLAPDLLGLFAMLFTVHFLDIRKNAPRFYKPYL
                     SLGTLVALALVLDIAGRPHDGLKLVQLAGVAFSIASMVVAVIVYRRGYKPARYYILGF
                     GAYLCGLTIFILLDAGVVPYSVFARHAAQVGAAIEAVLLSFAIGDKINRFKKEKEKAQ
                     QEALVAAQENERIVREQNVMLERKVEERTAEIRMQKAIIEEKNKDILDSIHYAKRIQR
                     ALLASDTLLQNRLPEYFVLYRPKDIVSGDFYWADETPEGKFLLLTGDCTGHGVPGAFM
                     SLLNISITHELTFGHRIFRPDLLLNAQRDAIIMSLNPEGSGEISKDGMDCVLCSYDFG
                     KMQMEFACANNPLWILRNGEMMEFKADKQPIGMHEGPKKEFTLHSVQLQKGDTVYTFT
                     DGFADQFGGPRGKKFKYSQLKELLLSIGGKTMEEQKNILEKTFSDWKGDLEQVDDVLV
                     IGVRI"
     gene            7552..>8074
                     /locus_tag="VFU15_07250"
     CDS             7552..>8074
                     /locus_tag="VFU15_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HEU4717611.1"
                     /translation="MKARTLRKNKVNVVTLGCSKNIFDSEVLMGQLKANNFDVEHESV
                     QEDSSIVIINTCGFIDNAKQESIDTILKFVDAKEQGLVEKVYVTGCLSERYKHDLEKE
                     IPEVDAWFGTRDLPRMLKTLKADYKHELVGERILTTPQHFAYLKISEGCDRPCSFCAI
                     PLMRGKHISTPIEQ"
BASE COUNT         2025 a         2160 c         2139 g         1750 t
ORIGIN      
        1 gctccagatc cagtcggtgc gccccattta caaatcgtcg tattcctcca cgatgatcaa
       61 tcacaacgac ccggacattg aattcaatta cgtgcagttc cagccgctgg aattttccat
      121 cagccagcac ctgaacaatc ttacctccat tctcgcgtat tacgcgtacg tgatccttgc
      181 gcaggattac gattcttatt cttccgacgg cggaacgcag tattggcaga aagcgcagac
      241 catcgtgaac aatgcgcaga acgccccgga aaaaggatgg aaatcgatgg agtcgaacac
      301 gcagaaccgt tattacctcg tggaaaacat gctccagccg atctacgagc ccgtccgcca
      361 gtgcaattac aaataccacc gcctcgggtt cgacatcatg tacaacgatg tgaacggggg
      421 ccgcgccatc tgcctggaag cgctcaacag cctcgaagcc gttcacgagc agcgcccgct
      481 ttcgttcccg atgcagttct ggttcaatgc caaatcgcag gagatcatca acctgttttc
      541 cggcgggttg cccgaagaga aaacaaaagt ggtcgctctt tgccagaaga ttgatcccgg
      601 tcacggcctg ctttaccaga agatcacgca gacccagtaa ttttttcctc ctgaatttta
      661 tcattgcgta gtctggtttt ttggtacaat tttggaagct cattccagaa aagatccatg
      721 aaaccgaaaa ttctacgccc gttcattttc atttttgtcg ttctgcttgc cggtgaagcc
      781 tgcaagatgt cttccgtgcg attgcaggtc ctgcaacccg cggatatcat gcttccgcag
      841 cagatacaga agttcgttct cgccgaccgt acgcgcccgg ccaaaggtgg cggtcagcag
      901 gcgctgaata tcgccgaagg aattctcacc ggcgaaggaa ttttccagga taaatggggc
      961 gcggaagatt gcctcgaggg attgcggcag gtgctgacga tgacgccgcg ctataccgtg
     1021 acgatcgcgt cgctggatac ggccctcaaa ggaacggggc gccgccagac catgccgccg
     1081 ctcgactgga ccacggtcgg aaagctggtc ggaaacgaca cgacgacggc gctcgttgtg
     1141 ctcgaggcct tcgattcaaa caccaatctt ttcaacgaag tggtgaacac cacccagcgc
     1201 ggacctgatg gcggcaacgt caccgttccc gaataccatg cacatggcaa agtggacgtg
     1261 tattgcgtgt ggagaatcta cgacctgcgt tcaaagacga tcgtcgatga gttcacgcag
     1321 gaaagctggc agcagttcga cggttccggc ccgacgctgc aggtggcgca gtcgcgcctt
     1381 cccaaccgcc gcgacatggt cagccgcgcc ggggtttccg ccggcatcca gtatggccat
     1441 cgtatttcgc cccagtggat ctgggttacg cgtgaatatt accgccgcgg ctccccgaag
     1501 ctcaagcagg cttcaaagct ggtgcattac aacaactgga aaacggccac ggatatctgg
     1561 aatgcggaag cttcgaatac cgatcccaag atcgccaagc gggccagctt caatatggcg
     1621 cttgccgccg aacaggccgg ccagcttgac cttgcgctgc agtgggccga ccgggccgtg
     1681 cagatgggcg ataagcgggc gccgcgttac gtccagatcc tgcagcagcg ccagtatgag
     1741 cagcagaagc tgcaggagca gatgaaagga aagcagcaat gacgggtaaa ttgaaatggt
     1801 tttttgttcc gaagcagctg ttttgaataa aaattcctac atttgccctc cattttcgaa
     1861 aacagagagt catgtcaaaa gtctgccaga tcaccggaaa acatacgatt gtcgggaaca
     1921 acgtttccca ctccaatcgc aaaacgaagc gcacgttcca gccgaacctg cactccaaga
     1981 aattctacct gcccgaggaa aagcgctgga tcaccctgaa agtttctgca gccgggctcc
     2041 gcacgatcag caagatcggc atcggcgaag cgctcaagcg cgcgaaagcc aaaggcctgg
     2101 tgaaattgaa ataacctgcc agggcagaac gcctttcaga aacttatttt tccatttgaa
     2161 aacgccgttc ccgaccggga tggcgttttt gtattttcga actgtcatga cgaaacgttt
     2221 cctgcttctt cccgccagct tttttctcgc cctcattttt ttctcgttca ccggcgcacc
     2281 ggatcattac gattacgaca ccgacctgct gacaccgcag ttccaccagg gcaggccgca
     2341 ggcgttgcgg gaccttcttc ccgacagcag cgtggctgtt ttttttgcaa gtcccgtgcg
     2401 cacgcgttcg aacgatgtgg aatacgatta ccaccaggac ccgaacctgt tttacctgac
     2461 aggactttgc gagccgaacg ccatgctcat cgtgttcaag gaaaaacagc aggtggatac
     2521 gatgttcacg aatgaactcc tcttcgtaca ggaccgtgac ccgaaagaag aagcgtggac
     2581 cgggaaacgc ctgggcgctg acggcgccaa acagctgctg ggaatagaca aggtgctttc
     2641 ctcgaaagat ttttctcctt ccatgataaa gtggaaaaaa ttccacacgg tgttcttttt
     2701 cccgctcacc ggtgaaatga acgatcaccc ggacgatccg tgcgatcttt cgctgctgct
     2761 cgcgaaattt tccaacagca cgaagcggaa cagcaacgac gacaccgaac gcctttcgga
     2821 atacatggcc atgctgcgcg aagtgaagca accggaagag ctcgagctga tgcgcaaggc
     2881 gatcctcatc agcggcgacg ggcatacgga actgatgaaa gcgctcgagc ccgacatgac
     2941 agaataccag gcgcaggcca tactcgaatt ccggttcaaa atggaaggcg ctgaatttcc
     3001 ggcttacccg agcatctgcg gatcgggaga aaattcctgc attcttcatt acacgtcgaa
     3061 caggaggcct ttccaggacg gcgatctcat cgttgtcgat gccggtgccg aataccacgg
     3121 ctattgcgcg gatatcacac gcacgcttcc tgtgaacggg aaattttccg ccgagcagaa
     3181 gcagatctac gacatcgttt acgatgcgca gcaggccggg atacaggcat gcaggatcgg
     3241 caacgaattc cgcgcgccgc acaatgcagc tgtggaggtg atcaaaaaaa ggctgctggc
     3301 gctcggcatc atacagaacg aagaggatta catccgctat tttttccacg gaacttcgca
     3361 ttatctcggg cttgatgtgc acgatgccgg gctgaacgga aggcttgccc tcgggaacgt
     3421 gatcacggtt gaaccgggga tctatattcc cgccggttct ccctgcgatc cgaagtggtg
     3481 gaacatcggt gtccgcatcg aggatgatgt gcttatcact tcgggcgatc cggaagtgct
     3541 ttccgggaat attccgaaaa cgacgaccga cattgaggcc atgatggcgc agaaaagcta
     3601 cctgaacgaa agtggcgggc cgaggtaatt aatttgttat caattaattg tattgataag
     3661 ggtgattcgg caaacagaat tcaatcatta ttccgttccc cgctattgca ttggagatca
     3721 aaaccattat ctttgcatcc catttataaa aagaggacca aatggctaag aaaggcaatc
     3781 gcatacaggt aatcctggaa tgcaccgagc acaaggaaag cggaaagccg ggaacttcgc
     3841 gttatatcac gacgaagaac aagaaaaaca cgcccgaccg catcgagctc aagaagtaca
     3901 acccgatcct gaaaaaagtg acggttcaca aagagatcaa gtaaacgacg ttcaataatt
     3961 ttttgtcatg gcaaagaaag tagttgctac gctcaagacc ggtgaaggaa aacagttcac
     4021 ccgcgtaatc aagatggtta agtctgataa gaccggcgcc tattcgttcc gggaagaagt
     4081 ggttcacaac gaccacatca aggatttcct ctctgaaaaa aaataatcac cccgcgatga
     4141 cgggtcacga agcttctttc ctgcgaaaga agctttcgtt gtttctgaac tgaacagtat
     4201 gggcatcttc ggatttttca gcaaagagaa gaaagaaagt ctcgacaaag gactttcaaa
     4261 gacgaaggaa agcttccttt cccgcattgc gcgcgccgtg gtggggaaat ccacggtcga
     4321 ttcggaagtg ctggacaatc tcgaggaaat acttgtcacc tcggatgtag gcgtggacac
     4381 gacgctgaag atcatcaaac gcatcgagga ccgcgtgtcg aaggacaagt acgtcaacac
     4441 ttccgagctg gacaagatcc ttcgcgacga gatcgccgcg ctgctggctg aaaacaagtc
     4501 ggaagacctg caggattttt ccgttcccga agggaaaaag ccgtacgtga tcatggtcgt
     4561 cggtgtgaac ggcgtgggaa aaaccacgac gatcggcaag ctcgcgaacg cattcaaaaa
     4621 atcaggaaag agcgtaatgc tcggcgctgc ggatacattc cgcgccgcag ccgtcgacca
     4681 gctcacgatc tggtcgcagc gcgtggatgt tcccatcgtt gcgcagggca tgggagccga
     4741 tccggcttcc gtcgctttcg atacgctgaa ttccgcggtg gcgaaaggtt ccgacgtcgt
     4801 catcatcgac acggccgggc gcctgcacaa caaagtgaac ctgatgaacg agctcaccaa
     4861 gatcaaaaag gtgatgcaga aagtcgttcc cgacgcgccc cacgaagtgc tgctcgtgct
     4921 cgacgggtcg accgggcaga acgcgatcga gcagtgcaaa caattcaccg cggcaacaga
     4981 cgttacggcg ctcgccgtta ccaagctcga cggcaccgcg aaaggcggcg ttgtcattgg
     5041 catatccgac cagttcaaaa ttcctgtgaa gtatatcggc gtcggcgaaa agatcgacga
     5101 cctgcaggtg ttcaacaaga ccgagttcgt ggattcgctg ttcaggaagg attgagtttc
     5161 ctgttttcag aaacgaattt caggatttcc cactaaacat attcgctcct gccgcccggg
     5221 accggtgtgc cgctcattat ccgggcgctg cctgcgaacc gttttcaaag aactatttca
     5281 aaaaataaac ggcggaaaat tgaatatcca cctgtttctc ttcctgctgt tttcgccggg
     5341 tctatcctga aaatcctacc tttcacgtga attcgcgttc gaatttatgc tgcgtaaatc
     5401 catcctcgtt tttgctttgt tgctggcccc gttcttcctt gcggcgcagg atgtagttgt
     5461 gctgaaggac aacgcacccg tcacttccct ggtggggcac tacgaagtgt tcgtggattc
     5521 caccgggaat atgtcgctcg gcgaggtgat gcattccggg aagttttaca aacccctgca
     5581 gccgaacccg aatttcggca tcacttcttc cgctatctgg gcacggttca cgctgaagaa
     5641 aaccgggaag gcgcgacagc ttttttctta tgggaactgc ggctccgatt ccgtcatact
     5701 atacgaaacc ggcggaccaa caacagggaa acagatcatg agcggccgtg acagttaccg
     5761 gaaacgtgaa ttcggtgtca acctggcgct tttttccctt gctattccgg cggacagcac
     5821 aagaacgttt tacctgcgtg tgaaagatgt tccgcccgtg atgatggtgc ttgaagccgg
     5881 aacggaggaa agctatttca cgtatgcatc gctttacaac ctgctgaacg gcgtgttcta
     5941 cggcatcatt ttgctgatga tcatctacaa cctgttcctt ttcttcacca accgtgatcc
     6001 cgtttacatc tattatgttg tttacacgct cgcgaacggg ttgtttatcg ctttcgtcaa
     6061 cggctatctt atttattttc cgcactggct gaacagcttg tttttccggc tgcatacgct
     6121 tgcgcccgac ctgctcggcc tgttcgccat gcttttcacg gttcattttc tcgacatcag
     6181 gaagaatgcc ccgcgatttt acaaacccta tctttcgctt ggcaccctcg tcgcgctcgc
     6241 gctggtgctc gacatcgcag gaagacccca cgacggcctg aagctcgtgc agctggccgg
     6301 cgtcgctttt tcaattgcct cgatggttgt ggccgtgatc gtgtacagga gaggttacaa
     6361 gccggcgcgg tattacattc tcggtttcgg cgcgtacctg tgcggactca ccattttcat
     6421 cctgctggat gcgggcgtcg tgccgtacag tgtttttgcg cgacacgcag cacaggtggg
     6481 agcggcgatc gaagcggtgc tgctttcttt cgcgatcggg gacaagatca accggttcaa
     6541 aaaggaaaag gaaaaagcgc agcaggaagc gctcgtggcc gcgcaggaaa atgaacgcat
     6601 tgtccgggag cagaacgtca tgctcgagcg gaaagtggaa gaacgtacag cggagatccg
     6661 catgcagaaa gccatcatcg aagaaaagaa caaggacata ctcgacagca tccattacgc
     6721 gaaacgcata caacgtgcat tgcttgcatc ggacacgctg ctgcagaaca ggctcccgga
     6781 atatttcgtg ctgtacaggc cgaaagacat tgtcagcgga gatttctact gggcagacga
     6841 aacgccggaa ggaaaattcc tgctgctcac tggcgactgc acggggcatg gcgttcccgg
     6901 cgcgttcatg tcgctgctga acatttccat cacgcatgag ctgacattcg gtcaccgcat
     6961 tttccgtccg gatctcctgc tcaatgcgca gcgcgatgcg atcatcatgt cgctgaaccc
     7021 ggaaggctcc ggggaaattt cgaaggacgg catggactgt gtgctgtgca gctatgattt
     7081 cgggaaaatg cagatggaat tcgcatgcgc caacaatcct ttgtggatcc tgcgtaacgg
     7141 ggaaatgatg gaattcaaag ccgacaagca gccgatcggc atgcacgaag gcccgaagaa
     7201 agaatttacg cttcactccg tacaactgca gaaaggcgac accgtttaca cctttacgga
     7261 tggtttcgcc gaccagtttg gcggaccgag gggaaagaaa ttcaagtaca gccagctgaa
     7321 agagctcctg ctttccatag gcggaaaaac gatggaagaa cagaagaata tcctcgaaaa
     7381 aactttcagc gactggaaag gcgacctcga gcaggtggat gatgtgctgg tgatcggcgt
     7441 gagaatctga cgaagtcaga gagtgaacgc cagagcacca catcccgaaa cgtcggtggt
     7501 gctggtggtc ggtgtgagaa tctgagtctt ttggttctta cctttgcagc gatgaaagcc
     7561 aggactctcc gcaagaacaa agtgaatgtc gtaacgctcg gctgctcgaa aaacattttc
     7621 gattccgaag tgctgatggg acagctgaaa gcgaataatt tcgacgtgga gcacgaatcc
     7681 gtgcaggaag attcatccat cgtcatcatc aacacgtgcg gcttcatcga taatgcgaaa
     7741 caggaaagca tcgacacgat attgaagttc gtagacgcga aagagcaggg gctggtggaa
     7801 aaagtttacg tcaccggctg tctcagcgaa cggtataagc acgacctcga aaaggaaatt
     7861 ccggaagtgg atgcatggtt cggcacgcgc gaccttcccc gcatgctgaa aacgctgaaa
     7921 gccgattaca agcacgagct cgtcggggaa aggattctca ccacgccgca gcatttcgct
     7981 tacctgaaaa tttctgaagg ctgcgaccgt ccgtgctcct tctgcgccat cccgctgatg
     8041 cgcggcaaac acatttccac gccgatcgaa cagc
//
LOCUS       DASTSF010000250         7557 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_540192,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000250 DASTSF010000000
VERSION     DASTSF010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 7557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7557
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_540192"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..722
                     /locus_tag="VFU15_07255"
     CDS             <1..722
                     /locus_tag="VFU15_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015809125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717612.1"
                     /translation="SSTEEQVVSSVSQQEETDIVSQQEEVRNAAVSEEVIAGTQTEEQ
                     KETILPVSNADVEFEITNTSSGSDEISMESEENWNEENEQEFSMNVVETQESYNEVSA
                     ELNAPENNSEVNEPVSEWKKTEEPEMKVEEQASEIPAVSAEEEARTRAEEEKKQAAEE
                     IQRKAQERIARLKELSLKLKTPQGLKEMEHEPAYKRRNIQLDNVPHSSESQVSRFTLT
                     ENEDKKIEIKPNNSFLHDNVD"
     gene            783..1229
                     /locus_tag="VFU15_07260"
     CDS             783..1229
                     /locus_tag="VFU15_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEU4717613.1"
                     /translation="MTLEEKVQNDIKSAMLAKDQARLEALRAIRSAMLLLKTSPEGLT
                     DDSAIKAMQKEVKKRRETAEIYKTQNRDDLADVELFQAGVIEEYLPKQMDEAALRSAL
                     QQLITEVGASSPADMGKVMGAATKKFAGVADGKMISLIVKEILSKS"
     gene            complement(1361..1957)
                     /locus_tag="VFU15_07265"
     CDS             complement(1361..1957)
                     /locus_tag="VFU15_07265"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEU4717614.1"
                     /translation="MKKLYAISLLAGLGFAFSANAQCTISSATITPGGLTVNANMVAS
                     GAAAPVYGWDWGDQTSPSTTQSATHTYTAAGTYTVCAVYVDGSNFTCYDSTCQTITVS
                     ATGISDPSVSAVNVKATPNPFSSNVQLTVTLDQPENVSMSVYDLTGKQVAVIQNGLMP
                     AGSNMINWTPEGLSEGVYFLQVKAGDAVTTKKLVYTAQ"
     gene            complement(2066..2683)
                     /locus_tag="VFU15_07270"
     CDS             complement(2066..2683)
                     /locus_tag="VFU15_07270"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEU4717615.1"
                     /translation="MSRTINPSSMRLRPVFLFVLQIFSTSFLGAQCSVSITSSNANGM
                     TLDVTATGTGSSHPFYVWSWGDNSNVSFNATDSHTYVANGTYTACVTYTDSLNPSACI
                     DTACITVTVPVTGMRGENRDATALSVAPDPFDGSTLAAIDLASPAEDAEISIYDVSGR
                     CIAVVFSGQLDAGRHTFPVSLQAPGIYFLSAQIDGITRVQKMICR"
     gene            complement(2667..2990)
                     /locus_tag="VFU15_07275"
     CDS             complement(2667..2990)
                     /locus_tag="VFU15_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017715374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEU4717616.1"
                     /translation="MKIYAIDRILPTATEEKIRGVVIREALHTWILYSKELIREVYFR
                     KDRPGVIIALECANVEEARNILSTFPMVRSGVIDFDIIPVGHFVPFGTLFDQELLKSV
                     NEQNH"
     gene            complement(3084..3923)
                     /locus_tag="VFU15_07280"
     CDS             complement(3084..3923)
                     /locus_tag="VFU15_07280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717617.1"
                     /translation="MKRFLLPYLLFFLSAEIHGQIAHDTAGCRFFQPFAGYKSWTSPA
                     KGTFYLTWGYNKDAYTKTDVRVHSSDEKVDFTLYDMTARDHAQWKDILRVQPSIPQYD
                     YRLGYWLPNGKFGIEANFDHAKYIVNDWQHVHMNGNIYGEHFDTDTTVDPDHFLHLEH
                     TDGANLLMLNLMFRAPLFASTHAHLFWIAKAGAGVVIPRSDVTLWGLRWNHCFHIAGP
                     TTGIETGFRLEFYKYFFFEPTIKGAYCDFKNVLARDNILISHQFFAGMGILCAGFQVP
                     LGK"
     gene            complement(4157..5785)
                     /gene="groL"
                     /locus_tag="VFU15_07285"
     CDS             complement(4157..5785)
                     /gene="groL"
                     /locus_tag="VFU15_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654643.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEU4717618.1"
                     /translation="MAKNISFDLEARDALKRGVDALSNAVKVTLGPKGRNVVIDKKFG
                     SPAITKDGVTVAKEIELKDPVENMGAQMVKEVASKTADVAGDGTTTATVLAQAIVTAG
                     LKNVAAGANPMDLKRGIDKAVESVIESLSKMSKKVGDDNEKIEQVATISANNDNAIGK
                     LIAEAMGKVKREGVITVEEAKGTETTVEVVEGMQFDRGYLSAYFVTDADKMEAVLDSP
                     LILIYDKKISNMKELLPVLEKAVQTGKPILIIAEDVDSEALATLVVNKIRGSLRICAV
                     KAPGFGDRRKAMLEDIAVLTGGTLISEERGFKLENADLSFLGKAEKITIDKDNTTIVG
                     GAGKKADITARVNQIKAQIENTTSDYDKEKLQERLAKLAGGVAVLYVGAATEVEMKEK
                     KDRVDDALHATRAAVEEGIVPGGGVAYIRAQESLDKLKGANEDEGTGIQIIRRALEEP
                     LRQICANAGVEGSIVVQKVRDGKADFGYNARTETYEKLYDAGVIDPTKVARVALENAA
                     SIAGMLLTTECVLSEIREEKPAMPPMGGGMEGMM"
     gene            complement(5812..6090)
                     /gene="groES"
                     /locus_tag="VFU15_07290"
     CDS             complement(5812..6090)
                     /gene="groES"
                     /locus_tag="VFU15_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019597634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEU4717619.1"
                     /translation="MANLKIKPLADRVVVEAAAAEEKTAGGIIIPDTAKEKPQRGKVI
                     AIGTGKKDEPLTVKVGDNVLYGKYSGTEIQIDGKDYLIMRESDIFAIV"
     gene            complement(6267..6650)
                     /gene="secG"
                     /locus_tag="VFU15_07295"
     CDS             complement(6267..6650)
                     /gene="secG"
                     /locus_tag="VFU15_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005865837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEU4717620.1"
                     /translation="MSAVISVLILLCCIFLVLAVLVQNPKGGGLASGFTSGAQVMGVR
                     GTADFLEKATWTIAIVLFVISVFSTGMAAKAKNQGGEASVTKDKAAEFNTKPNAGGSG
                     NAFPINNANPPAANAPAPVPAGGQK"
     gene            complement(6663..>7557)
                     /locus_tag="VFU15_07300"
     CDS             complement(6663..>7557)
                     /locus_tag="VFU15_07300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU4717621.1"
                     /translation="TSPDEWEAVMMRQLQLLHHWKTTPEELIRNQEAEKKISPAAEEK
                     PAVTEEKTETTEEKSPTAADEINTLLYVLVEPGGAEDVNDTETVQEPVAEEIPENGEE
                     WEVTVVNEESPAEEEEPVHPVAVPEDPVTQEIFREAISSSIGLEVDDTLPAPEELGFV
                     ATNIPAVAEENEPGPEKEQAEEQTGELSFSQWLMSVSRTPAEQKPVTETGNASGLVEK
                     FLREQPRIQPKKSFYNPVNMAKKSVQESDEFITETLARIYVKQGNFTKAIRAYQKLSL
                     KFPEKSAYFAALIEELKRTPK"
BASE COUNT         1664 a         1925 c         2059 g         1909 t
ORIGIN      
        1 tttcttcaac ggaagagcag gtggtcagtt ccgtatcgca gcaggaagag acggatatcg
       61 tttctcagca ggaagaagta cgcaacgctg ctgtttcgga agaagtgatc gcaggtacgc
      121 aaacggaaga gcagaaggaa acgatcctcc cggtttcgaa cgctgatgtg gaattcgaga
      181 tcacgaatac gtcttccggc agcgatgaga tctccatgga atcggaagag aactggaacg
      241 aggaaaatga gcaggagttc agcatgaacg tggttgaaac gcaggaatcg tacaatgaag
      301 tttctgccga actgaatgcc ccggaaaata attccgaagt gaatgaacct gtttccgaat
      361 ggaaaaaaac ggaggagccg gaaatgaaag tggaagagca ggcttctgaa ataccggctg
      421 tttccgcgga agaagaggcg cgcaccaggg cggaggaaga aaagaaacag gcggctgaag
      481 agatccagcg caaggcgcag gagcgtatcg cccgtttgaa agagcttagc ctgaaactga
      541 aaacgccgca gggactgaaa gagatggagc atgagccggc ttacaaacgc aggaacatcc
      601 agctcgacaa cgttccgcat tcttcggaat cgcaggtttc ccgtttcacg ctgacggaga
      661 acgaggacaa gaagatcgag atcaagccca acaattcttt tctccacgat aatgtagatt
      721 gatcaggagg gaatcggaac acgggtccgg ttgcgttgga ccggcccgtt ttttagcagt
      781 atatgacact cgaagaaaaa gttcagaacg atataaaatc agccatgctg gcgaaagacc
      841 aggcaaggct ggaagcgttg cgggccatca ggtccgcgat gctcctgctg aaaacttcgc
      901 ctgaaggatt gacggatgat tctgcgatca aagccatgca gaaggaagtg aaaaagagaa
      961 gggagacggc cgagatctac aagacgcaga accgggatga tcttgcggat gtggagcttt
     1021 tccaggcggg tgttattgaa gaatacctcc cgaagcagat ggacgaagcc gcgctgcgaa
     1081 gtgcattgca gcagctgatc acggaggtag gggcatcttc accggccgac atgggaaaag
     1141 tgatgggcgc cgctacaaaa aaattcgccg gcgtagccga tggaaaaatg atctccctga
     1201 ttgtaaagga aattctttcg aagagttagt gtgttgtaaa ttgtgtgatt gtgtgtggga
     1261 acgtccggct ggtgtgtggc cggacgttct gtttttgtaa atgtgaacac aaaaaaaacg
     1321 gctcccgcgg gaaccgtttc tcattgaaaa agtgaaatga ttattgcgct gtgtaaacga
     1381 gtttcttcgt cgtcaccgca tcaccggctt tcacctggag gaaataaacg ccttcggaaa
     1441 gtccttccgg tgtccagttg atcatgttgc tgccggcagg cataagaccg ttctggataa
     1501 cagcaacctg tttcccggta aggtcataca cgctcatcga aacgttttca ggctggtcaa
     1561 gcgtaaccgt cagctgaacg ttggatgaaa aaggattcgg tgtagccttt acgttcaccg
     1621 ccgatacgga aggatcgctg atgcccgttg cagaaacggt gatcgtctgg caggtggaat
     1681 cgtagcaggt gaagttggaa ccgtccacgt aaactgcgca aacggtgtac gtaccggccg
     1741 cagtgtaggt gtgcgtagcc gactgcgtcg tggaagggct tgtctgatcg ccccagtccc
     1801 agccataaac aggtgcagct gctccgctgg cgaccatgtt cgcatttaca gtaagtccgc
     1861 ccggggtaat ggttgccgag gaaatcgtgc actgcgcgtt ggcgctgaat gcgaaaccga
     1921 gtccggcaag aagtgaaatc gcgtagagtt ttttcatttg tttttgtttg gttttggtag
     1981 gattcaaaaa taggaaggtc gcttcagaat gtcaattaaa aggagccgac tttcgcacgc
     2041 tcatttgtta aaacagcacg aagttttaac ggcagatcat tttctgcacc cgtgttattc
     2101 catcgatctg tgcggacagg aaatagatcc ctggcgcctg cagggaaaca gggaatgtat
     2161 gccggcctgc atccagctga ccgctgaaga caacagcgat gcagcggcct gaaacgtcat
     2221 aaatggaaat ttctgcatct tctgccgggg atgcaagatc tatggcagcg agcgtgctgc
     2281 cgtcaaacgg atccggcgcc accgacagtg ccgtcgcatc ccggttttcc ccgcgcatgc
     2341 ctgtaaccgg gaccgtaacg gtgatgcagg ctgtgtcgat gcaggccgaa ggattgagcg
     2401 aatccgtgta cgtgacgcac gcggtatacg ttccgttcgc gacgtacgtg tgcgaatcgg
     2461 ttgcgttgaa gctgacgttg ctgttgtcgc cccacgacca tacatagaac gggtgtgaag
     2521 aacctgttcc tgttgcggta acgtcgagcg tcatcccgtt tgcgttcgaa gaagtgatcg
     2581 aaacggaaca ttgtgcgcca agaaaagatg tggaaaatat ctgcagtacg aaaagaaaaa
     2641 caggacgcag ccgcatggac gaaggattaa tggttctgct cattcacgct cttcagcagc
     2701 tcctggtcga acagcgtgcc gaacggaacg aaatgtccca ccgggataat atcgaaatcg
     2761 atcacgcccg aacgcaccat cgggaaagtg gaaaggatgt tacgcgcctc ttcaacgttc
     2821 gcacattcca gcgcaatgat gacgccggga cggtccttgc ggaaataaac ttcgcggata
     2881 agctctttcg aatacaatat ccatgtgtgc agcgcttccc tgatcacaac gccgcggatc
     2941 ttttcttccg tggcagtcgg taatatcctg tcgatggcgt agatcttcat gtcgtttatt
     3001 tttttaaaag gtagatatta ttcgtgaatc ccgaccggtt tgcctcatac ctgtgatgct
     3061 aaaacgccgg gagtacgatg atattatttg cccagcggaa cctggaaacc cgcgcagaga
     3121 attcccatgc cggcgaagaa ctggtgactg atcaggatgt tgtcgcgcgc cagcacgttc
     3181 ttgaaatcgc agtacgctcc cttgatcgtc ggttcgaaga aaaaatattt gtaaaactcg
     3241 agacggaacc cggtttcgat gccggtcgtc ggacctgcga tgtgaaagca gtgattccac
     3301 cggagtcccc atagtgtaac atccgatcgc gggatcacga cgccggcgcc tgctttcgcg
     3361 atccagaaaa gatgtgcatg cgtcgaggca aaaagcgggg cgcggaacat gaggttaagc
     3421 atgagcaggt tcgctccgtc cgtatgctcg agatgaagga aatgatcagg gtcgaccgtc
     3481 gtgtcggtgt cgaaatgttc tccgtaaata tttccgttca tgtgtacgtg ctgccagtcg
     3541 ttcacgatgt atttcgcgtg atcgaaattg gcttcgatgc cgaatttccc gttcggaagc
     3601 cagtagccga gacggtaatc gtactgcgga atgctcggct gaacacggag gatgtctttc
     3661 cattgcgcat gatcgcgtgc cgtcatgtcg tacaatgtga aatccacttt ctcgtcggag
     3721 ctgtgaacgc ggacgtccgt ttttgtataa gcgtccttgt tgtagcccca ggtgaggtag
     3781 aacgttcctt tcgcgggaga tgtccaggat ttgtaaccgg caaatggctg gaaaaaacgg
     3841 cagcctgccg tgtcatgtgc gatttgtccg tgaatttctg ctgagagaaa aaacaggaga
     3901 tagggtagaa ggaatcgttt cactttttgg gattttccct tcgccgggaa ctgtcgatga
     3961 ttaataacat gaagaatgga agggcgtgag cgccgatgag gacaggggga aagccgcaca
     4021 gctttgcaat tacggcgccg gtgaaagctg ccgcaattcc gatgagcgct attttccgca
     4081 tcttgctcat ggcttaaaga taacagaaaa gccccgcgga tttccgcagg gctttcttct
     4141 attttccggg tgaaaattac atcatccctt ccatgccgcc gcccatcggg ggcatagccg
     4201 gcttttcttc cctgatctcc gaaagaacgc attccgtcgt gagcagcatt ccggcgatgg
     4261 acgctgcgtt ctcgagcgca acacgcgcca ctttcgtggg atcgataacg cctgcgtcat
     4321 acagtttttc gtacgtttcg gtgcgtgcgt tgtagccgaa atcagctttc ccgtcgcgga
     4381 ctttctgcac aacgatggaa ccttccacgc ctgcgttcgc gcagatctgg cgcagcggct
     4441 cttcgagcgc acggcggatg atctggatgc ctgtgccttc gtcttcgttc gcgcctttca
     4501 gcttgtcgag cgattcctgt gcacggatgt acgcaacacc gcctccggga acaatacctt
     4561 cttcaaccgc agcgcgcgta gcgtgcagcg catcatccac gcggtctttt ttctctttca
     4621 tttccacttc cgtcgcagca ccgacgtaaa gaacggcaac accgcctgcg agcttcgcaa
     4681 ggcgctcctg cagtttttcc ttgtcgtaat ccgacgtggt gttctcgatc tgtgctttga
     4741 tctggttcac gcgtgcagtg atgtcggctt ttttgccggc accgccgacg atcgtcgtgt
     4801 tgtctttatc gatcgtgatc ttttctgctt ttccgaggaa agaaagatcg gcgttctcca
     4861 gcttgaaccc gcgttcttcg ctgatgagcg ttccgccggt gagcactgcg atgtcttcga
     4921 gcatggcttt gcggcgatca ccgaagccgg gcgctttcac cgcgcagatg cgcagcgagc
     4981 cgcggatctt gttcaccacg agcgtagcga gcgcttcgct gtccacgtct tccgcgatga
     5041 tcaggatcgg cttgccggtc tgaaccgctt tttcgagaac aggaagaagc tctttcatgt
     5101 tggagatctt cttgtcgtaa atcagaatga gcgggctgtc gagcacggct tccatcttat
     5161 ctgcgtcggt gacgaagtaa gcggaaagat agccgcggtc gaactgcatg ccttccacca
     5221 cttcgacggt ggtttcagtt cctttcgctt cttccacggt gatcacgcct tcgcgtttca
     5281 ctttgcccat ggcttcggcg atgagcttgc cgatcgcgtt gtcgttgttc gcggagatcg
     5341 tcgccacctg ttcgatcttc tcgttgtcgt cgccgacttt cttcgacatt ttgctgaggc
     5401 tttcgataac agactcaacc gctttatcga tgccgcgctt gaggtccatc gggttcgcac
     5461 ccgcagccac gtttttcagt ccggcggtta cgatcgcctg tgcaagcacg gtggcagtcg
     5521 ttgttccgtc accggccaca tcggcggttt tggaagcgac ttccttcacc atctgcgcgc
     5581 ccatgttttc cacgggatct ttcagctcga tttcttttgc tacggtaacg ccgtctttcg
     5641 tgatggccgg cgagccgaat tttttatcaa taaccacgtt gcggcccttg ggaccgagcg
     5701 tgacctttac tgcgtttgac agggcgtcaa cgccacgttt cagtgcgtcg cgcgcttcga
     5761 gatcaaatga gatattctta gccatttttg aaatttttta aagtgaagga attaaacgat
     5821 ggcgaaaatg tcggattcgc gcataatgag gtaatctttt ccgtcgatct ggatctctgt
     5881 tccggaatat tttccataaa gaacattgtc gcccactttc acggtaagcg gctcgtcttt
     5941 ttttccggtt ccgattgcga tgacttttcc ccgttgcggt ttttctttcg ctgtatcggg
     6001 aatgatgatt ccgcctgctg ttttttcttc cgcagctgcg gcttctacga ccacgcggtc
     6061 ggccaaaggt ttaatcttca gattagccat ttctattcgc tttgattttg gttaaacaat
     6121 gtgtgtgata tgaatttccg gacagcccct atcgaatttt atgccagccg aaacgggctt
     6181 gctttccacg gccatttttt cagtttttac caatgaaaaa tgccaggaaa tgacatcctg
     6241 acatttacgt ttttttgcgc gaagcattac ttctgtccgc cggcaggaac cggtgcaggt
     6301 gcgtttgctg cgggaggatt ggcgttgttt atcgggaaag cattcccgct gccgcctgcg
     6361 ttcggcttgg tattgaattc ggccgctttg tcttttgtta cggaagcttc accaccctgg
     6421 ttcttcgctt tggctgccat gccggtggag aaaacgctga tgacgaacag cacgatcgcg
     6481 atcgtccacg ttgctttttc aaggaaatcc gccgtgccgc gtacgcccat cacctgcgcg
     6541 ccggacgtaa acccggaagc gagtccgccc cctttgggat tctgtaccag tacagcaagc
     6601 acgaggaaaa tgcagcagag aaggatcaat acggaaatta ctgcgctcat ttcagatagg
     6661 aattatttgg gtgttctttt cagttcttca ataagggctg caaagtaagc gcttttttcc
     6721 ggaaatttca aactcagttt ctggtaggcg cggatcgctt tggtgaagtt gccctgcttg
     6781 acgtagatac gggccagcgt ttccgtgatg aattcatcgc tttcctgcac gctctttttg
     6841 gccatgttga ccgggttgta aaagctcttt ttcggctgaa tacgaggctg ttcccgaagg
     6901 aatttttcca ccaggcccga cgcgttccct gtctccgtga caggtttctg ctccgccggc
     6961 gtcctggaaa ccgacatcag ccattgcgag aagctgagct ccccggtttg ctcttcggcc
     7021 tgctcttttt cggggcccgg ttcattttcc tccgccacgg cgggaatgtt cgtcgccacg
     7081 aaaccaagct cttccggcgc cggaagcgta tcgtccacct ccagcccgat cgatgagctg
     7141 atcgcttccc ggaaaatttc ctgcgtcacc gggtcttcgg gaacggcaac ggggtgaacc
     7201 ggttcttcct cctccgcggg agactcctcg ttcaccactg tcacttccca ttcttccccg
     7261 ttctccggaa tttcttccgc gacaggctcc tgcacggttt ccgtatcatt tacatcttcc
     7321 gctccgcccg gttccaccag cacgtacaga agtgtattga tctcgtcggc tgccgtcggg
     7381 gatttttctt cagttgtttc tgttttttct tcggtaacgg ctggtttttc ttcagcggcc
     7441 gggctgattt tcttttccgc ttcctggttg cggatgagct cttccggtgt tgttttccag
     7501 tgatggagca actgcagctg gcgcatcatc actgcttccc attcatccgg gctggtg
//
LOCUS       DASTSF010000251         2841 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1539058,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000251 DASTSF010000000
VERSION     DASTSF010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2841
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1539058"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..248)
                     /locus_tag="VFU15_07305"
     CDS             complement(<1..248)
                     /locus_tag="VFU15_07305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717622.1"
                     /translation="MKKVFTSAWFNLLLGILLAALTLIAFRHDAGRAVENVMWNAEGR
                     PEPMCFTWFFLTGYILHSLYMTGLSVYYFVRKKWFTPL"
     gene            515..1003
                     /locus_tag="VFU15_07310"
     CDS             515..1003
                     /locus_tag="VFU15_07310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717623.1"
                     /translation="MKKIYTAFLALVIAGGLSAQAPANQKSPQNNSGSGGTTTDDNQP
                     SNCYTKWEKIFEERGAEDVKDGVYDDVIITVRKGADASCYSGKVEVKDGKIINMYRKL
                     SDGTYELYKPKLQFEKPMTIVNGISVSLLTVDDELVNVIFPKTLKPKKADYTNAPDPP
                     GD"
     gene            complement(1076..1696)
                     /locus_tag="VFU15_07315"
     CDS             complement(1076..1696)
                     /locus_tag="VFU15_07315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717624.1"
                     /translation="MKWFKYPLAIVAYGLLGVFLFAELWHYVGKSEVESFWYYFPSFA
                     GVFLLASFILSKLKLPWEGDVMVQVAMVLAPVLWFVNNAPPAKQPVYVFYVKPDFQGT
                     LQVHFDHSDNAETKVRSTADTLFFRFDENGEIMLNEDFRTVRESVMQRLYYLNEDKSR
                     VHITGLDAKKTPSDTALVYVREDSIRSDKGKIDAMFFRVDKSQHLK"
     gene            complement(1729..1983)
                     /gene="purS"
                     /locus_tag="VFU15_07320"
     CDS             complement(1729..1983)
                     /gene="purS"
                     /locus_tag="VFU15_07320"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016195127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HEU4717625.1"
                     /translation="MKFKAEIDVMPLKALLDPQGKAVTGSMKNLGLPEIQNVRIGKHI
                     TLEIDADNRDAAHAKVDEACKKLLANQIMEFYEFELSEIR"
     gene            complement(2119..>2841)
                     /locus_tag="VFU15_07325"
     CDS             complement(2119..>2841)
                     /locus_tag="VFU15_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717626.1"
                     /translation="MLGAAFAVYYPDANSLAQSGTGPFGNLWTTFNANAPKELTWMDF
                     APFIAFLIPLFSALRLAKFNNDKRQTDSFIGVPTPANSILICSFVLILRFSRLWIADW
                     PADIGGAPGPSYARKIFNLFMQSPPANAGLRVNGGWAPGVSFAESLLLNPWFLVAVTL
                     VMSFLLVAEIPLFALKFKNFGWKGNEVRYLFLILSVALAALLQFTGVPLVIVLYVLLS
                     VVNNAMKKKSGGAPGGAETPGS"
BASE COUNT          726 a          819 c          716 g          580 t
ORIGIN      
        1 aacagcggcg tgaaccattt tttgcggacg aaataataaa cggaaagtcc ggtcatgtaa
       61 agagaatgca gaatatagcc ggtcaaaaaa aaccaggtaa aacacatagg ttccggcctg
      121 ccctccgcgt tccacataac attttccacc gcccgtcctg catcgtgcct gaaagcgatc
      181 agcgtcagcg cagcgagcaa aattcccagc agcaggttga accaggcgga cgtaaacact
      241 tttttcacgc ccggccaatt taggacaatc gcggccaatc ggttacaaac gcttatttcg
      301 caggtgaaac gtttaatccc gggaacgggc gccggaagac aaccttgcag gttataacct
      361 tgcaggtacc ccatacacag aaaaccccaa aacccgaaac tgaaaaccct caaccgtcaa
      421 cctttcggcc cgcatttttt cggtatggat tttgttatct ttaccattac cccggccatg
      481 gttccgggaa tgaccaacca actcacctta attcatgaag aaaatataca cggcgtttct
      541 agctctcgtg atcgccggcg gactgtcagc acaggcaccc gctaaccaga aaagtcccca
      601 gaacaactcc ggatccggcg gaacgaccac cgatgacaac cagccttcca actgctacac
      661 gaagtgggaa aaaatcttcg aagagcgcgg tgcggaagac gtgaaagacg gcgtgtatga
      721 cgacgtgatc attaccgtac gcaagggcgc tgacgcttcc tgctactcgg gaaaagtgga
      781 agtgaaagac gggaagatca tcaacatgta ccgcaagctc agcgacggaa cgtacgagct
      841 gtacaagccc aagctgcagt tcgaaaaacc gatgacgatc gtgaacggta tttccgtttc
      901 actgctcacc gtcgacgatg agctcgtgaa cgtcatcttc cccaaaacac tcaagccgaa
      961 aaaagcggat tataccaacg ctcctgatcc cccgggcgac taaaacgcat ttcaaaaatt
     1021 ccggatcccg ggtacctgct tcaggcatgt gcccgggatt ttttttgaga ccgggctatt
     1081 tcaggtgctg cgacttgtcc acgcgaaaga acatcgcgtc gatctttccc ttgtcggaac
     1141 ggatggaatc ttcacgcaca tacaccagcg cggtgtcgga aggcgttttc ttcgcgtcga
     1201 ggccggtaat gtgcacgcgc gatttatcct cgttgaggta atacagtctc tgcatcaccg
     1261 attcgcgcac cgtgcggaaa tcttcattga gcatgatctc cccgttctca tcgaagcgga
     1321 agaaaagcgt atcggccgtg ctgcgcactt ttgtctccgc gttatccgaa tgatcgaaat
     1381 gcacctggag cgttccctgg aaatccggct tcacgtaaaa aacataaaca ggctgcttcg
     1441 ccggcggggc gttgttcacg aaccagagca cgggcgccag caccatcgcc acctgcacca
     1501 tcacatcgcc ttcccagggc agcttcagct tcgaaaggat gaacgacgcc aggaggaaca
     1561 caccggcgaa agaagggaaa taataccaga agctttccac ttcacttttc cccacgtaat
     1621 gccacagctc cgcaaacagg aagacgccga gaagtccgta ggcaacgatc gcaagtggat
     1681 atttaaacca tttcatggcg ctaatcccgg ttcagaacgc atacgcgctc accggatctc
     1741 cgacagctcg aactcgtaaa actccatgat ctggttcgca agcagttttt tgcaggcttc
     1801 atccactttc gcatgcgccg catccctgtt gtccgcatct atctcgagcg tgatgtgctt
     1861 cccgatgcgc acgttctgta tctccggcag ccccaggttt ttcatggaac ccgttaccgc
     1921 ttttccctgc ggatcgagga gcgccttgag cggcatgacg tcgatttctg ctttgaattt
     1981 catcgatgat gagttgataa gttgaaaagt gaatggcaaa attaacgatt tggggcggac
     2041 ctgcagtgcg ttgtatttac tccgttcccg tgttccactg caacgctgcc gcgttccgct
     2101 tcacccgggg ccagggcatc agcttcccgg cgtttcagct cctccgggcg caccgccgct
     2161 tttcttcttc atcgcattgt tcacgacaga cagcagcacg tacagcacga tcaccagcgg
     2221 aacaccggtg aactgaagca gcgccgccag cgccaccgac aggataagaa acaggtagcg
     2281 cacttcgttc cctttccacc cgaaattttt gaacttgagc gcaaacagcg gaatttccgc
     2341 caccagcagg aaagacatta ccagcgtgac agcgaccagg aaccacgggt tcagcagcag
     2401 cgattcggca aaagaaacac ccggcgccca cccgccgttc acccgcagcc ccgcgttggc
     2461 aggcggcgac tgcatgaaca ggttgaatat cttccgggca taagacgggc ccggcgctcc
     2521 cccgatgtca gcgggccagt cagcgatcca cagcctgctg aagcggagga taaggacgaa
     2581 tgaacaaatg agaatggaat tggccggcgt cggcacaccg atgaacgaat cggtctggcg
     2641 cttgtcgttg ttgaatttgg ccagccgcaa cgcagagaac agcgggatca ggaaggcaat
     2701 aaaaggcgca aaatccatcc aggttaattc cttcggtgca ttcgcattga atgttgtcca
     2761 gagattcccg aacgggccgg tgccggactg cgcgagggaa ttcgcgtcag gataataaac
     2821 ggcgaatgcg gcgccgagca t
//
LOCUS       DASTSF010000252        12697 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1580077,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000252 DASTSF010000000
VERSION     DASTSF010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 12697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12697
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1580077"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..588)
                     /locus_tag="VFU15_07330"
     CDS             complement(<1..588)
                     /locus_tag="VFU15_07330"
                     /inference="COORDINATES: protein motif:HMM:NF024257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEU4717627.1"
                     /translation="MRRPIFPYVLPAVFFTAVLFASCRRGENGNANIKPVDTSVVMKG
                     SESEFNLMEYMKNEFTKKNPRISVTLSGGGSAVGIGGLIRGENNIANASRKITDGEVA
                     LAMSNQVNPIPVIIATDAIAIITHPAVCVDSLSMQQLSDVFSGKITNWKEVGGADLPV
                     KVIGRNQNSGTRYFLMDHLRMNGFAPGTTVEQDNPQ"
     gene            956..3160
                     /locus_tag="VFU15_07335"
     CDS             956..3160
                     /locus_tag="VFU15_07335"
                     /inference="COORDINATES: protein motif:HMM:NF040146.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HEU4717628.1"
                     /translation="MKKFLTLVSALIAFSVTGFSQVNIQAARQAAINSTVTIRGIVTN
                     GAELGTIRYVQDSSAGIALYDAALSGVQRGDSIVATGTLTSYDSLLEVQPVSTHTVVT
                     TGNTLPAPVVLTPSQIGEAVEGELVRVNNATFQTGGTFAGNTSYTFTSNSQSGTVYVR
                     TGSPLVGAIIPTGYVSIIGVASQYFQTYQLLPRDTADLISNSSISIITPLTQSNMTAS
                     GFNISWNTNITGSTYVLYGRTPALELGMLSGASGLTHTVTVSGANPSDIFYVKAYSVN
                     GSDTAFSATRAFATVSASSGRITVYFDKSVDTTLSTGVNATLLFHTIDDTIAAYINRA
                     KYTIDIAMYSFDNTNIVNLSTALNNAYSRGVRVRVIYDGSQTTAAVSQLNPNIPIVYS
                     PTTSAYNIMHNKFMIIDALSTDPGDPLLWTGSTNWTDNQTGTDANNVIIFQDQSIAKG
                     YKIEFDEMWGDTGMVPNAGNSRFGPYKTDNTPHEYLIGGHRVEQYFSPSDGTNQQIVN
                     HLGTANTDLCIATMIITRSDLAYAITGASSSGVQTYVMVDDQTTTTVWPTLSAALPAG
                     HLVDYSANGIMHHKYAIIDPNNVASDPMVMTGSHNWSTTADTKNDENMVIVHDDTIAN
                     IYYQEFHPRFTQNGGTIIGIETADQNAPGFRAWPNPSMDVCNIGFTLTSSENVSVTVT
                     DISGNIISTSSVAGMTGYNTTQIGTGGLPAGIYFVHVTGATVSSTCRIVVGH"
     gene            complement(3290..6406)
                     /locus_tag="VFU15_07340"
     CDS             complement(3290..6406)
                     /locus_tag="VFU15_07340"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEU4717629.1"
                     /translation="MKRRLRAATVFFCLSAGTFTGLKAQSFDWAQQSGWGDDNQYGLD
                     VAADDSGNVYNAGFVTGYTTIFDQTGDVEFDNTGSSTTEDGFVVKYDKNGLAEWVIQV
                     GSTGNDRCVGVSSYQNGPEDPNIYVTGTFNGTATITDGHGSTSTITSSGGQDAYIAKF
                     DGNGNFIWAYDIGGSGTDYAYDVSGNADNQGHSHVLYIAGRNASGTGMKCYYSGGSYI
                     SPVSYYSGEAGYLLKYVDNGTTGTASWIRITDSSNPDSYYCVGSSTSQVSVGGYIRGN
                     ATVQANGSGGTSSLTLSGAYDIAVASYDVSGTLVWTHRYGGSSLLTENATCLTMDNDN
                     NTYVGGYYGYSGSPFSCNNGGWEGLVMAIDPSGTTLWNSCGGSSGDDYVSGIAIDKCS
                     KHVYVGGIYKGSFTWGSASLSPFNSTADNDNFMLTLDASDGSYMSGTANRLGGIGNDV
                     LYAMTANGLNHIYLTGTSTSGTWKIGSTSYFTNSNTPTGNYDAFIGRWIDGGSYPSKP
                     SSGSASHRGVDVLDCDVYAGGTMTGSVTFGSVSHTSTNNSIGNPSDDIYMTKSDRFGT
                     YNSFVQVAYGTSNEDMTGQVQDASGNLYLSGYANTGSAGNGVTFNTSPSATTVTFTNG
                     GPQAMVCQTDPNGVTQWYAFGIAGSGAQAWANDVALDASGNVYICGSFQGTVTFGSTN
                     LTASSQEDAFIAKYNSSGTLQWAKDVANGTSSGITVRAFGICVDGSGYVYITGTYGGL
                     TQFNGNMVSIPLNAGSNGNDIFVAQYDASGWALKALGYAGSTGWDEGRRVTAVNSQAI
                     YVTGYSNISSQKTFTGKCTMSSTPSWSWTKNATGSLSYGQGIVTDGTDLYVTGYGNNG
                     TVFGSFTLSLSYGSPFLVRYNGNNGTEEYASAREVGSYAGGMAIALDDETPDNAFIAG
                     TYPTLSSYYAFIDQLSTSNGNYARHANPFTVQPAQDNSAISSASVFPNPSYDGILNVK
                     IDGTTGDDAPASILIYDLTGQLVLRKDGITTAQEAIDAGMLDDGTYLYEVVRENNRIG
                     NGKIVIAK"
     gene            6650..8170
                     /locus_tag="VFU15_07345"
     CDS             6650..8170
                     /locus_tag="VFU15_07345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717630.1"
                     /translation="MHSVYHLVAVFSAQERLRLHNALQPDKTKGENKYLQLFEFLSSC
                     SKPPSPGEASFAVYGSPADSKISKLAQRLRQKILDIIITESFLEANERLTHESAAWLS
                     AKRKAFQVSVLYNLAGSGASTVELTEEAIAEASSSGAHAQLLELYHYKRGTASFTAGE
                     KDFLQCTHKLQYHLKCFETNEKILGEYYRITNPQGFGRAEAAAEQAKKIKDFLDGIEK
                     DKEYVTGAQHNYLLCVIRTEYLLLCDRAEEAKMRLGEFMLNLSLSDSDNGIIKAKLHS
                     HIAACDLLSGNYDKSIAGYEHCLETFRNTNLFNYFVHLVSVFYPLFYSGRNAYAEKIV
                     NEIISGGRYPINDFRSEEPKYFLACIRFREREFSAALKILNGLSALAKDKTGYEIAIR
                     ILRIQCLLELQRPDEASPQIENLRKHVSRHKSKNYISERNRLITQVLICMDKRGFSGA
                     AEKQEAGLLEKLCSRERPLRWECRTPELIVFHHWYVDFHGHRNGRKTDRKKESQFQ"
     gene            complement(8236..>12697)
                     /locus_tag="VFU15_07350"
     CDS             complement(8236..>12697)
                     /locus_tag="VFU15_07350"
                     /inference="COORDINATES: protein motif:HMM:NF013636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HEU4717631.1"
                     /translation="IGNGSSATYGIQLSQQMNFAVFNNEIRNITGTGTSTVDGFVSTG
                     TSGNSSFHNNKIYTIKNTGTTSTANITGIRANVTSGSNSISIYNNFVADLSSGYTGAA
                     TATRVIRGIYVQTGGGGTGAEMINVGFNNVSIDNSSAPNVSSACYETGSATGPVMNVE
                     NNVFSNSTTAQAAPAGHYAYATPSATATGNTGSVSNYNDLYIANATQGFTGIGSATTF
                     ATLANWQAAMTQDANSIAVNPAFNGPVDLHVNAVSLDGAGTSIPGVTNDIDNDTRAAT
                     PDIGADEFTASATDVGAILLVTPVAGNCYSAAEPVVVRIQNFGTAALDFTTDNTTVTV
                     NVTGAVTQTLNITLTNNALNSGNPLASGATLDVPVGTLNMSSNGTYTFNANTTLATDA
                     NSINDAMPVVNITVNPGIVAVSTPSVCDGNSVTLTLSGNTNGTIQWQSSTDGGVTWVN
                     ETGPGNTSSPYMTAPSDTTMYRSLMCGLYVSDTVNVIWNAVTTPVTVNDTVCGMGQVT
                     LSASGSGTLTWYDAATGGNIVNSGTSYTTGVMSTTTYYVSNTSGGSVQTVGPVDNTFS
                     GGAQQLSGNYDIFDVYQSCVLQSVIVYPGSAGNVVIDLQDNTGTTIQQFTYAVTAGDV
                     NNPTLIPVNFNLTPGTNYRLTQDVSSVSMFRNSAPGNLYPYTLPGVLSITNSAAGNTF
                     FYFFYDWHILTGCESGRVPVSAVVIPSAVVTASATSSVICAGDSTQLTATSTNMNYAY
                     TWTPGMTVTDSTAAMTYAHPQMPATYILMADSANCHMEDTIMISVNTVPPVSVSMSDS
                     TICIGQQDSLIVNGISSAPFINPNPFAIPDASPAGVTSVISIPFSSTITTTSINRVCI
                     DISHTWDGDLAISLISPLGTQIDLSSNNGGSGDNYSGTCFKMSAATNITAGAAPFTGN
                     YLPEAAGGFNAFNGENTQGNWQLFVEDQAGGDVGTINQWMIQFNNTTVYSWSSNPPGF
                     TGTGDTAIVSPAATTQYMLTVTDTTTGCTHQYFTTVNVNAPLSVMVSGDTMICPADSS
                     FLVTSVTGGDGNYVYNWSDATTMANDTVVPASTTQYMVTVSDGCGTPVAGDSILVSVA
                     TPVMITSVTADTTVCSGTNVPVAVMISGGDDNIMYSWTGGGTMANDTVMSGMPGTSMN
                     YAIDVMDGCGTSASDTVMISAFNVPSVTAPADTTVCGGSTVVLMSAAAGGDGNYSYTW
                     SNGPVTANDTLTASSTMNYTVTVSDGCSNMGTDSAMVAVYSIPSVIVSSDTAICNGDS
                     VMLMANATGGDMNYMYMWSNSDTLAGTTVMPSTSTSYNVTVTDGCGNSAMDSAMVTVN
                     NGPVASFTQSSAGSTVTFSNTSTGATSYQWNFGDSQTSTSMSPSHTYAGNGTYTVTLI
                     ATNGCGSDTTTMVIVLDVGISEQAAALGIGAFPNPTHDMLNVVFNGQVSGNAVIDMYD
                     LEGRLIDSRQVNAVQPGTLVQMDVTTFVRGVYMMHVTVNNSTVVLKVIVQ"
BASE COUNT         2948 a         3489 c         3273 g         2987 t
ORIGIN      
        1 ctgcgggtta tcctgttcga cggtggttcc cggcgcaaag ccgttcatgc ggagatgatc
       61 catgagaaaa taacgcgtgc cggaattctg gttgcgcccg atcactttca ccggaagatc
      121 ggcgccaccg acctctttcc agttcgtgat cttcccgctg aacacatccg acaactgctg
      181 catggaaagg ctgtcgacgc aaaccgcggg atgcgtgatg atcgcgatcg cgtctgtcgc
      241 aatgatgacc ggaatggggt tcacctggtt gctcattgcc agcgcaactt caccgtcggt
      301 aattttccgc gacgcattgg cgatgttgtt ctcgccgcgg atgagtccgc cgatgccgac
      361 cgcagatccg ccgcccgaga gtgtcacgct tatgcgcgga ttttttttcg tgaattcatt
      421 cttcatgtat tccatcaggt tgaattccga ttcggatccc ttcatgacga cactcgtgtc
      481 gaccggttta atatttgcat ttccattttc gccgcggcgg caggaagcga ataaaactgc
      541 cgtaaaaaaa acagcaggga ggacgtacgg gaaaattggt ctgcgcatga ttacacgatg
      601 taagaacggc aagttgccgg acgcttttta atttaagccc cgcttcattt tatggattgg
      661 ttaattcaga caaaaccgcc attcctacgg atgaatgtgt cctattccct gcaccattcc
      721 ttgaacatac gttcccgtta cgaaaacatt atttttcctt tcaccctgtg cgcagatgga
      781 agaagcgttt atttttgaag ctcagaaaag ctgccgctga tcacctacag gacttccctc
      841 ccgggaacca ctccgaaacc ggcgcagctt gtgaaccgga taatcacaaa acccggcata
      901 attccgttcg aaaacggacc cgggtattca gcttcatcat tcacactttc ctcacatgaa
      961 aaaatttctg acactcgttt cagccctgat cgcattctca gtaaccggct tctcgcaggt
     1021 caacatccag gcggcgcggc aggcggcgat caattccacg gtaacgatcc gcggcattgt
     1081 caccaatggc gcagagcttg gcaccatccg ttacgtccag gattcttctg cgggcatcgc
     1141 gctttacgac gccgcgctct ccggtgtgca gcgtggcgac agtatcgtcg ctacgggaac
     1201 gcttacgagc tacgacagtc ttctcgaagt tcagccggtg agcacgcata ccgtcgtcac
     1261 cacgggcaat acgctgcctg cgcctgttgt cctcacaccc tcgcagatcg gcgaagcggt
     1321 ggaaggcgag ctggtgcgcg tgaacaatgc aacattccag acaggcggaa ccttcgccgg
     1381 caacacgagc tatacgttca cgagcaattc gcagagcggc actgtgtacg tacgcaccgg
     1441 gtcgccgctc gtcggcgcta tcattcccac cggttatgtt tcgatcatcg gggttgcatc
     1501 gcagtatttc cagacttacc agctgctgcc gcgcgataca gccgatctta tttccaacag
     1561 cagcatttcc atcatcacgc ccctgacgca atcgaacatg acggcttccg gtttcaacat
     1621 cagctggaac accaacatca cgggaagcac gtatgtgctg tacggacgca cgcctgccct
     1681 cgagctcggc atgctttccg gcgcttccgg gctgacgcac accgtaaccg tttccggcgc
     1741 taacccgtcc gacatttttt atgtgaaggc ttattccgtg aatggatccg atacggcatt
     1801 ttccgccacg cgcgcattcg cgactgtatc cgcgtcctcg ggaaggatca ccgtttattt
     1861 cgacaaatcg gtcgacacga cactttccac cggggtgaac gcgacgctgc tttttcatac
     1921 gatcgatgat acgatcgccg cgtatatcaa ccgtgcgaaa tacacgatcg acatcgcgat
     1981 gtacagcttc gacaacacga acatcgtcaa tctttccacg gcgctgaaca acgcttacag
     2041 ccgcggtgtg cgtgtgcgtg tgatctatga cggctcccag accacagcgg cggtgagcca
     2101 gctgaacccg aatattccca tcgtgtacag cccgacgaca tccgcctaca acatcatgca
     2161 caataaattc atgatcatcg atgcgctttc cacggatccc ggcgatccgc tgctgtggac
     2221 gggctccacg aactggacag acaaccagac aggaaccgac gcgaacaacg tgatcatttt
     2281 ccaggaccag tcgatcgcaa aaggatacaa gatcgagttc gatgagatgt ggggcgatac
     2341 gggaatggtg ccgaacgccg gcaactcgcg tttcggtccg tacaagaccg acaacacgcc
     2401 gcatgaatac ctgatcggcg gtcatcgcgt ggagcaatac ttcagcccgt ccgacggaac
     2461 gaaccagcag atcgtcaatc acctcggcac tgcgaatacg gatctctgca tcgcgacgat
     2521 gatcatcacg cgatccgacc tcgcctacgc gatcacgggc gcgtcgtctt ccggtgtgca
     2581 gacgtatgtc atggtggacg accagaccac aacgacggtc tggccgacgc tcagcgcagc
     2641 acttcccgcg ggacatcttg tcgactattc cgccaacggc atcatgcacc acaaatacgc
     2701 gatcatcgat ccgaacaacg ttgcatccga cccgatggtg atgacaggct cacacaactg
     2761 gagcacgacc gccgacacga agaatgacga gaatatggtc atcgtgcacg acgacacgat
     2821 cgcgaatatc tattaccagg aattccatcc acgcttcacg cagaacggag gaacgatcat
     2881 cggcattgaa acagctgacc agaatgcacc cggcttccgt gcgtggccga atccgtccat
     2941 ggacgtctgc aacatcggtt tcacgctgac gagcagcgaa aatgtttccg tcacagtcac
     3001 cgacatttcc ggcaacatca tttccaccag ttcggtcgcc gggatgaccg ggtataacac
     3061 tacgcagatc ggcacgggcg gattgcctgc gggaatttat ttcgtgcatg tgaccggtgc
     3121 gactgtttct tcaacatgca ggatcgtagt ggggcactga gccggaaggt tttcattcgc
     3181 gatcctgaat aatttcagat cattgaagat atctgtttca gaaaaatgaa agaaaaaagg
     3241 cgaagccatt acggcctcgc cttacggggt taatgaaaca tttccgcttt tacttcgcga
     3301 tcacgatctt cccgttcccg atgcggttgt tttccctcac cacttcgtac aggtaagttc
     3361 cgtcatcgag catgcctgcg tcgatcgctt cctgcgcggt cgtaattccg tctttccgga
     3421 gaacgagctg accggtgaga tcgtaaatga ggatcgaggc gggagcgtca tctcctgtcg
     3481 ttccgtcgat cttcacgttc agtattccgt cgtaggaagg attggggaat acggaggcgc
     3541 tgctgatggc cgaattatcc tgcgcaggct gaaccgtaaa cggatttgca tggcgcgcgt
     3601 aattgccgtt actggtcgac agctggtcga taaacgcgta atagctgctc agcgtaggat
     3661 aagtgccggc aataaaagca ttatccggcg tttcatcatc caaagcaatc gccattccgc
     3721 ctgcgtacga tcccacttcc cgcgctgatg cgtactcctc tgttccgttg ttgccgttat
     3781 acctgaccag gaaaggtgaa ccgtaagaca agctcagcgt gaacgagccg aacactgttc
     3841 cgttgttgcc gtaaccggta acgtagagat ctgttccgtc ggttacgatg ccctgtccgt
     3901 agctcagcga acctgtcgca tttttggtcc atgaccagga aggcgtggag gacatggtac
     3961 acttgccggt aaatgttttc tggcttgaaa tattggaata accggtaaca tatattgcct
     4021 ggctgtttac cgcggtgact ctgcggcctt catcccaacc cgtactgcct gcatagccga
     4081 gcgccttcag cgcccatccg ctggcatcgt actgcgcaac aaaaatatcg ttgccgttgc
     4141 ttcccgcgtt aagcgggatg ctgaccatat ttccgttgaa ctgcgtgagc ccgccgtatg
     4201 tgcccgttat atacacatac ccgcttccgt cgacgcatat cccgaatgcg cgcaccgtaa
     4261 ttccggaaga tgttccgttc gccacatctt tcgcccattg cagcgttccg gaactgttgt
     4321 atttggcgat aaacgcatct tcctgcgacg atgcggtaag attagtgctt ccgaacgtaa
     4381 ccgttccctg gaaggaaccg catatataaa cgttcccgga agcatcaagc gcaacatcgt
     4441 ttgcccaggc ctgtgcaccg gatccggcaa taccgaaagc ataccattgc gtaacaccat
     4501 tcgggtcggt ttggcagacc atcgcctgcg gcccgccgtt cgtaaacgta actgtcgttg
     4561 cagaaggaga cgtattgaag gtaactccat ttccagctga accggtattt gcataacctg
     4621 aaagatacag gttgccggaa gcgtcctgca cctggccggt catgtcctcg ttggaagttc
     4681 cgtaagccac ctgcacgaac gaattatagg tgccgaagcg atctgatttc gtcatgtaaa
     4741 tatcgtcaga cggatttcca atagagttat tggttgacgt atgcgaaacc gatccaaacg
     4801 ttacgctgcc ggtcattgtt cctccggcat aaacatcaca atccagcaca tccactcccc
     4861 tgtgcgacgc acttccgctt gaaggtttgg acggataaga accaccgtcg atccagcgcc
     4921 cgatgaacgc atcatagtta ccggtaggag tattggaatt ggtaaagtac gaagtgctgc
     4981 cgatcttcca tgtgccagac gtggaagttc ctgtcaggta aatgtgattc aacccgtttg
     5041 cggtcatcgc gtaaagaaca tcgttcccga taccacctaa acggttggcg gttccgctca
     5101 tgtagcttcc gtcgctcgca tcgagcgtga gcatgaagtt gtcattatcg gctgttgagt
     5161 tgaaaggcga gaggcttgcg cttccccatg tgaagctgcc tttataaatt ccacccacat
     5221 agacatgctt cgaacatttg tcgatcgcga tccccgaaac gtaatcatcg cctgacgaac
     5281 ctccgcacga gttccaaagc gtagtgccgg aaggatcgat cgccatgacc aggccttccc
     5341 atccaccgtt gttacagctg aaaggacttc ccgaatatcc gtagtatccg cccacgtacg
     5401 tgttattatc gttgtccatc gtgaggcagg ttgcgttttc tgtcagcagt gagctgcctc
     5461 cgtaacggtg cgtccacacg agtgtaccgc tgacatcata actggccaca gcgatgtcgt
     5521 atgctccgct cagcgtgagc gaagaagttc cgccggaacc gttagcctgt accgttgcat
     5581 ttccacggat gtatccgccg accgaaacct ggctggtgct ggaacctacg caatagtagg
     5641 aatcaggatt ggatgaatcc gttatgcgga tccagctcgc cgttcctgtc gttccgttgt
     5701 cgacatattt cagcaggtaa ccggcttcgc cggaataata ggacacggga gaaatgtacg
     5761 acccgccgga ataatagcat ttcattccgg ttccgcttgc atttcgcccg gcgatataga
     5821 gaacgtgcga atgaccctgg ttgtccgcat tgccggaaac atcataggcg taatccgtgc
     5881 cgctcccgcc gatgtcatac gcccagataa aatttccgtt gccgtcgaat ttcgcgatgt
     5941 atgcgtcctg tcctccggag gaagtaatcg tactggtgct gccatggcca tccgtgatcg
     6001 tcgctgttcc gttgaatgtt cccgtgacat aaatgttggg gtcttcggga ccattctgat
     6061 aggaagaaac accgacgcag cgatcgtttc ctgtgctgcc cacctggatc acccattctg
     6121 caaggccatt tttatcatac ttgactacaa acccgtcttc tgttgtggaa gaaccggtat
     6181 tatcgaattc aacgtcaccg gtctgatcga aaattgtagt gtagccggtt acaaatcccg
     6241 cgttgtacac attcccgctg tcatctgcag cgacatcgag accatactgg ttgtcatctc
     6301 cccaaccgga ttgctgggcc cagtcgaagg attgcgcctt taaacctgta aatgtcccgg
     6361 cggaaagaca aaagaatact gtggcggcac gtagccgtct tttcatactg ctgttcatga
     6421 taagtaagat tttaactaat gaataattct gtgttttgag gtgacaggct caaggtttcg
     6481 cgaaacgacc tgcaaaacga aatacttaaa atcaatctat aattctatgg acattttata
     6541 ggttaaattg acatctcctg ttttattata caactgatta ttattcttat tttggaatac
     6601 cctacactaa ttgatacaat catgaatata acccttaaca accccgctta tgcattcggt
     6661 ttaccatctg gtcgcggtat tttccgctca ggaacgcctc cgcctgcaca acgctctcca
     6721 acctgacaaa acaaaaggag agaacaagta tctgcagctt ttcgaattcc tttcttcctg
     6781 cagcaaacca ccttcaccgg gcgaagcgtc gtttgctgtg tacgggtctc ctgccgactc
     6841 gaagatcagc aagctggcgc agcggttgcg ccagaaaatt ctcgacatca tcatcacgga
     6901 aagttttctc gaggcaaatg aacgcctgac ccatgaaagt gcggcatggc tcagcgccaa
     6961 acgaaaagcg ttccaggtaa gtgtgctgta caaccttgcc ggttccggcg cgagcacggt
     7021 ggaacttaca gaagaagcga tcgcggaggc ttcttcttcc ggggcgcacg cacaactgct
     7081 cgaactctac cattacaaaa gagggacagc atcgtttacc gccggtgaaa aagattttct
     7141 gcagtgcacg cacaagctcc agtatcacct gaaatgtttt gaaacgaatg aaaaaattct
     7201 cggagaatat taccggatca ccaaccccca gggctttggc agagcagagg cagctgctga
     7261 acaggcaaaa aaaataaaag atttcctgga cgggattgaa aaggataagg aatatgttac
     7321 cggcgctcag cacaattatc ttctctgcgt catccgcacg gaatatcttt tactctgtga
     7381 tcgcgctgaa gaagccaaaa tgagactcgg ggaattcatg ctgaaccttt ctttatcaga
     7441 ctcagataac gggatcataa aagcgaagct gcattcacac attgcggcct gcgatctgct
     7501 ttccgggaac tacgacaaat cgatcgccgg ttacgaacac tgcctggaaa cgttccggaa
     7561 tactaacctg ttcaattatt tcgttcacct cgtttccgtt ttttatcctt tgttttattc
     7621 cgggcgcaat gcttacgctg aaaaaattgt gaatgaaatt atctccggcg ggaggtatcc
     7681 catcaatgat ttcagaagcg aggaacccaa atattttctc gcgtgcatcc gtttccgcga
     7741 acgcgaattt tccgcggcgc tgaaaatcct caatggactg tccgcgctgg ccaaagacaa
     7801 aaccgggtac gagatcgcca tacgtattct ccgcatccaa tgcctcctcg agctgcagcg
     7861 cccggacgag gcttccccac agatcgaaaa cctccgcaag cacgtgagcc ggcacaaaag
     7921 caaaaactat atttcagaac gcaaccggct gatcacgcag gtgctcatct gcatggacaa
     7981 acggggattt tccggcgcag ccgaaaaaca ggaagccggc ctcctggaaa aactgtgttc
     8041 ccgggaaaga ccgctgcgat gggaatgccg gacaccggaa ctgatcgtgt tccaccactg
     8101 gtacgttgac tttcacgggc acaggaacgg ccggaaaacg gacaggaaaa aagaatcgca
     8161 gtttcaataa cggcgaaaag cagcacaaaa aaggaccggg atgacccggt ccttttcatc
     8221 ttataaaata ccagcttact ggacgatcac cttcagcaca accgtgctgt tgttcaccgt
     8281 cacgtgcatc atgtacacgc cgcgtacgaa cgtggtgaca tccatctgca caagcgtgcc
     8341 gggctgaact gcgttcacct ggcggctgtc gataagacgt ccttcaagat cgtacatgtc
     8401 aataactgcg ttacctgaaa cctggccgtt gaagacaaca ttgagcatgt cgtgcgtcgg
     8461 gttcgggaat gcaccgattc cgagcgcagc agcctgttcg ctgataccaa cgtcaagcac
     8521 gatcaccatc gttgtcgtgt ccgatccgca gccgttcgtc gcgatgagcg tcacggtgta
     8581 ggtgccattg cctgcatacg tatgcgacgg gctcatcgag gtggatgtct gcgagtcgcc
     8641 gaaattccac tggtaagaag tagcgcctgt cgacgtgttg ctgaacgtca ccgtcgatcc
     8701 cgctgagctc tgcgtgaaag aagcgacagg tccgttgttc acggttacca ttgcgctgtc
     8761 cattgcagaa tttccgcagc cgtccgttac agttacattg tacgacgtgg acgtggaagg
     8821 cataaccgtc gtgcccgcca gcgtatcgct gttgctccac atgtacatgt aattcatgtc
     8881 accgccggtc gcattcgcca tcagcataac gctgtcgccg ttacagattg ccgtgtcgga
     8941 agaaacgatc acggaaggaa tggagtaaac ggccaccatt gctgaatcgg ttcccatgtt
     9001 gctgcatccg tcgctgacgg ttaccgtgta attcatcgtc gatgatgcgg taagcgtatc
     9061 attcgccgtc acaggtccgt tgctccaggt gtacgaatag ttgccgtcac cgcctgcagc
     9121 ggcagacatc agcactactg tgcttccgcc gcaaacagtc gtatccgccg gggccgttac
     9181 ggaaggcacg ttgaatgcag agatcatcac cgtgtcgctt gcgctggttc cgcagccgtc
     9241 catgacatcg atcgcgtagt tcatgcttgt tccgggcatt ccggacatga ccgtatcatt
     9301 cgccatcgtt ccgccgccgg tccagctgta catgatgttg tcatcaccac cgctgatcat
     9361 taccgcaaca ggaacattcg ttccgctgca gaccgtcgta tcggcagtta ccgaagtgat
     9421 catcacgggc gtcgccacgc tgacgagaat agaatcgccc gccaccggtg ttccgcatcc
     9481 gtcgcttacc gttaccatgt attgtgttgt cgatgcagga acgacggtgt cattcgccat
     9541 tgtcgtcgca tcactccagt tgtacacgta gttgccgtca ccgccggtta cggacgtcac
     9601 caggaatgag ctgtccgccg ggcagatcat cgtatcaccg gaaaccatta ccgaaagcgg
     9661 ggcattaacg ttcaccgtgg taaagtactg gtgcgtgcag cccgttgtcg tatcggttac
     9721 agtaagcatg tactgcgtgg ttgcagcagg agatacgatt gccgtatcgc ccgttccggt
     9781 gaatcccggg ggattcgaag accagctgta aacagtcgtg ttgttgaact ggatcatcca
     9841 ctggttgatc gttccgacgt caccgccggc ctggtcttcc acgaagagct gccagttacc
     9901 ttgtgtattc tcgccgttaa acgcgttgaa tccaccggca gcttccggga gataatttcc
     9961 ggtgaacggg gccgctcctg ccgtgatatt cgttgccgcg ctcatcttga agcaggttcc
    10021 ggaataatta tcaccggaac cgccgttgtt cgagctcagg tcgatctgcg ttcccagcgg
    10081 ggaaataagc gagatcgcaa gatcaccgtc ccaggtgtgg gaaatgtcaa tgcaaaccct
    10141 gttgatgctg gtcgttgtaa tggtgctcga gaacggaatg ctgatcacgc ttgtcacacc
    10201 tgcaggactc gcatcgggaa ttgcaaaggg attcgggttg atgaacggag cggatgaaat
    10261 tccgttgacg atcagcgaat cctgctgacc gatgcagatc gtggagtcgc tcatgcttac
    10321 tgaaacaggg ggaaccgtat tcacggagat catgatcgtg tcttccatgt gacagttcgc
    10381 agaatcagcc atgaggatgt acgtcgccgg catctgcgga tgtgcgtacg tcattgcagc
    10441 ggtagaatcc gtaaccgtca tgccgggcgt ccaagtatac gcgtagttca tgttcgtgct
    10501 ggtcgctgta agctgtgtgc tgtcgcctgc gcagataaca gaagaagttg cgctggcagt
    10561 aactaccgcc gaagggatca caacggcgct taccgggaca cgtccgcttt cacaaccggt
    10621 aagaatgtgc cagtcgtaga agaaatagaa gaacgtgtta cctgccgcgc tgttggtgat
    10681 cgacaaaacg ccgggcagcg tatacggata aagattaccc ggggcgctgt tacggaacat
    10741 cgatacactg ctaacatcct gcgtaaggcg gtagttggtt cccggcgtca ggttgaagtt
    10801 gacaggaata agcgtcgggt tattgacatc gcctgccgtc actgcatagg tgaactgctg
    10861 gatcgtcgtt cccgtattat cctgtagatc gatcaccacg ttgcctgcgg aacccggata
    10921 aacgataacg ctctgcagca cgcagctctg gtaaacatcg aaaatatcat agttacctga
    10981 cagctgctgg gcgccgcctg agaatgtatt atcgacagga cctaccgtct gcacgcttcc
    11041 gcccgaagta ttggaaacat aatacgtcgt cgttgacata acgcccgttg tgtaagacgt
    11101 tccgctgttg acgatgttgc cgcctgttgc agcgtcatac catgtaagtg ttccgcttcc
    11161 cgaagccgag agtgtaacct ggcccatacc gcaaacggta tcgttcaccg ttaccggggt
    11221 tgtaaccgcg ttccagatca cgttcaccgt atcggaaaca taaaggccgc acataagcga
    11281 acggtacatc gtggtatccg acggagcagt catatacgga gaagacgtgt tgccgggacc
    11341 ggtttcattc acccaggtaa caccgccgtc ggtgctgctc tgccactgga tggttccgtt
    11401 ggtatttccg ctcagcgtga gcgttacgga attgccgtcg caaacagaag gagtggacac
    11461 cgcaacgatt ccgggattca cggtaatgtt cacgaccggc atcgcgtcgt tgatgctgtt
    11521 ggcatcagtc gcgagcgtcg tgttcgcatt gaacgtgtac gttccgttgc tgctcatgtt
    11581 gagcgttccc acgggaacgt ccagcgttgc gccggaagcg agcgggttgc ctgagttgag
    11641 tgcgttgttc gtaagcgtga tgttcagcgt ttgcgttacc gcaccggtta cgttgaccgt
    11701 aacggtcgtg ttatccgtgg taaaatcaag agccgcagtt ccgaaattct ggatacgaac
    11761 gacaaccggc tcagctgcgg aataacagtt tccggcaacc ggcgtaacca gcaggatcgc
    11821 acccacgtca gttgctgaag ccgtgaactc gtcggccccg atgtccggtg ttgccgcacg
    11881 cgtatcattg tcgatatcgt tcgtcacacc ggggatcgaa gtacctgccc cgtcgagcga
    11941 aaccgcgttg acgtgaagat caaccgggcc attgaaggcc gggttcaccg cgatggaatt
    12001 cgcatcctgc gtcatggctg cctgccagtt cgccagcgtg gcgaacgttg ttgctgagcc
    12061 gatgcccgtg aatccctgtg tcgcgttcgc gatgtaaaga tcattgtagt tggaaacgga
    12121 accggtgttg ccggttgcag tggctgacgg cgtggcgtaa gcgtagtgac cggcaggagc
    12181 cgcctgggct gtggtggaat tcgagaagac gttattctcc acattcatca ccggtcctgt
    12241 tgcagaacct gtttcatagc aggcgctgga aacattcggc gcagaagagt tgtcgatcga
    12301 tacgttgttg aacccgacgt tgatcatttc cgctcctgtt ccgccgccgc ccgtttgaac
    12361 ataaattccg cggatcacgc gcgttgctgt tgccgcacct gtataaccgg agctgagatc
    12421 agccacaaaa ttattgtaga tgctgatcga attcgatccg ctggtcacgt tcgcacggat
    12481 acctgtaata tttgcagtgg acgtcgtgcc ggtattttta atcgtgtata tcttattatt
    12541 atggaagctg gagttgcctg acgttcccgt gctgacgaag ccgtcaaccg tagaagtgcc
    12601 ggttccggtg atattccgga tctcgttatt gaaaacagca aaattcatct gctggctcag
    12661 ctgtatgccg tacgtggcgg aagagccgtt gccgata
//
LOCUS       DASTSF010000253         3619 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1606935,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000253 DASTSF010000000
VERSION     DASTSF010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3619
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1606935"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1174)
                     /locus_tag="VFU15_07355"
     CDS             complement(<1..1174)
                     /locus_tag="VFU15_07355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717632.1"
                     /translation="MEIEVACSATTFTGTMPIPSSGSWGNYPYYHSTLNPPGPEDCVL
                     EPYFPCSIPFSQLCPGTTYYWRAREFVEGSSSAGPWSAAQPFTTPGSPPVTTLVATAS
                     QYDACPGDMIQLNASVTGGCPGSFFSYSWSPTVGLSNPNIANPVLTVQAASTVYTVTV
                     TGGCVTITSADDTVAITNGPPPITGSPTANPPSICSGGSSVVTLSGQDPSSTIQWQTS
                     TNGVNWFNISGATSSSYNTGPMTSTLYYQAIVTGSGWPAGSGCGTNTSPPVIVTVNPS
                     PAADAGANTTVCTGACTTLTGSGGVSYVWQPGNLNTQSINACPVATTTYSLTVTDANG
                     CTGTDQVTVSISNASVTASPDVSICTGNSTVLVASGPAGNTYSWSPSGTLSGANTAN"
     gene            1522..>3619
                     /gene="rnr"
                     /locus_tag="VFU15_07360"
     CDS             1522..>3619
                     /gene="rnr"
                     /locus_tag="VFU15_07360"
                     /inference="COORDINATES: protein motif:HMM:TIGR02063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEU4717633.1"
                     /translation="MPSKKKSSPKALFEEVLDALNSSRGKALNYKQIASILGIKNPEE
                     RPLVLSILNDFVKKGLAEEPERGKFMAKNSAKTVDGTIDITQSGAAYVSSPGQKEDIF
                     IAARNTFGALHGDLVRVKVFQRKRSDGRDEGEVSGILQRSRTEFAGVIHFEKRTAFFV
                     PDNRKFPDFLIAPDKAGKARDGQKVVVRMTDWEDYSDLPNGEVVAVLGEPGNNETEIN
                     AIMVEYGLPYHFPKDVERAAGLIPVEITKEEISRRRDFRGVLTFTIDPFDAKDFDDAL
                     SVKKLPSGNWEVGVHIADVSHYVKPGSILDKEAVLRGTSVYLVDRVVPMLPEVLSNFV
                     CSLRPNEEKLTFSAVFELDENAKVVSEWFGRTVILSDRRFTYEEVQEILEGKPGDYAE
                     ELHELDKLAKKLRDDRMKNGSITFEREEVKFHLDENGNPTGVYFKVMKDSNQLIEDFM
                     LLANKRVAQYGALGRTTGEAKKKQGDLRRPFVYRIHNSPDPERVAEFSTFVKNFGYHM
                     NTGSEKAVSQSLNKLLKDVQGKPEANMVETLAIRTMAKAIYSTDNIGHYGLGFAYYTH
                     FTSPIRRYPDVMAHRLLQYYIDLDAGKNAGPAPDENELSSLCKHSSDREKLAAEAERA
                     SIKYKQVQFLQDKVGQVFDGIISGVTEFGFFVELEGNKCEGMVHVRNLKDDRYFFEEE
                     SYCLRGQRYGKKFTLGD"
BASE COUNT          907 a          908 c          998 g          806 t
ORIGIN      
        1 ggtttgctgt attggcccct gagagcgttc ccgacggcga ccagctgtat gtatttccgg
       61 caggacctga agcgacaagc accgtgctgt ttcctgtaca aatgcttaca tcagggctgg
      121 ccgtaacaga cgcatttgaa atgctgacgg ttacctggtc tgttcctgtg caaccgtttg
      181 cgtcggtaac cgtaagcgag taagtcgttg ttgcaaccgg gcatgcgttt atgctttgtg
      241 tgttaagatt tcccggctgc cagacatagc ttacacctcc cgacccggtg agcgtggtgc
      301 acgcgccggt acaaacggtt gtgtttgctc ctgcatcagc tgcgggagaa gggttcactg
      361 ttacaataac cggtggcgat gtgttcgttc cacaacctga gccagcaggc caacctgagc
      421 cagtaacaat cgcctggtaa taaagcgttg acgtcatcgg gccggtgtta tagctggagc
      481 tggtggctcc ggaaatatta aaccagttta ctccattggt ggatgtttgc cattgaatgg
      541 tggaagacgg gtcctggccg gaaagcgtga caaccgaact tcctcccgaa caaatcgagg
      601 gcgggttggc agtcggagaa cccgtgatcg gaggcgggcc attggtaatg gcaactgtat
      661 cgtcagcaga ggtgatcgtc acgcagcctc cggttaccgt aaccgtgtaa accgtgctgg
      721 cggcctggac ggtaagaacc gggttcgcaa tattgggatt gctgagccca accgttgggc
      781 tccatgaata ggaaaagaac gaacccgggc atccaccggt cacgctcgcg ttcaactgga
      841 tcatgtcgcc cggacaagcg tcatactgcg acgctgtcgc cacaagagtt gtgactggcg
      901 gagatccggg tgttgtaaat ggttgtgcgg ccgaccatgg tcccgccgaa ctgctccctt
      961 ccacaaattc ccgcgcccgc cagtaatagg ttgtccccgg acatagctgg gaaaatggga
     1021 tcgaacaggg aaaataaggc tccagcacac agtcttcagg tccaggagga ttgagcgtgc
     1081 tgtggtagta aggataattt ccccagctgc cgctggaagg aatcggcatg gtaccggtga
     1141 atgtcgttgc cgagcaggcg acctcgatct ccatccagta gggcccgcaa ccgcacgttg
     1201 caggatctga gtttccgtta atcgttaagt ctgttgcaga cgcggttgcc gtcaacccta
     1261 caagcgctac accatgacat gcatgagcat attttgcaga cgagaaaacc aggatagccg
     1321 cgaggaaaaa gagcctttta aaacgattaa gaaaatcgta atttttttcc atgttgtttt
     1381 gtttttgcca tttgtacaag tccgtactcc acggtcttgt caaaacataa cggcaaagct
     1441 ctgaaaagtt ttggaattat ttgaacaaat gttgataatc cgccatttcg acgggttatt
     1501 cggataattt tagccgatct tatgccatcg aagaaaaaat catccccgaa agcgctgttt
     1561 gaagaggtac tggacgcgct gaactcctca cggggtaaag ccctgaatta taagcagatt
     1621 gcttccattt tgggtataaa aaatccggaa gagcggccat tggttctttc cattcttaat
     1681 gattttgtta aaaagggcct ggcggaggag ccggaacgcg gaaagttcat ggcaaaaaat
     1741 tcagcgaaaa ccgtcgacgg caccattgac atcacacaat ccggagcggc ttacgtttct
     1801 tcgcccggac agaaagagga tattttcatc gcagcgcgga atacgttcgg tgcgctgcac
     1861 ggcgaccttg ttcgtgtaaa ggtcttccag cgcaaacgga gcgacggacg cgacgagggc
     1921 gaggtttccg ggattctgca gcgatcgcgg acggaatttg ctggcgttat tcattttgaa
     1981 aaacggacag ccttttttgt tcccgacaac cggaagtttc ccgatttttt aattgcaccc
     2041 gataaagccg gcaaggctcg tgacggacaa aaagtcgttg tgcgcatgac ggactgggaa
     2101 gactattccg atctgcccaa cggggaagtt gtcgcggtgc tcggcgagcc gggcaacaac
     2161 gaaacggaga tcaatgcgat catggtcgaa tacggtcttc cgtatcattt cccgaaagat
     2221 gtcgaacgtg cggcagggct tatcccggtt gagatcacga aagaagagat ctcccgcagg
     2281 cgggatttcc gcggcgtgct gacattcacc atcgatccgt tcgacgcaaa ggatttcgac
     2341 gatgcgcttt cggtgaaaaa acttccgtct ggcaactggg aagtgggcgt gcacatcgca
     2401 gatgtttcgc attacgtgaa gccggggtct attctcgaca aagaagccgt gctccgcgga
     2461 acgtccgttt accttgtcga ccgcgtggta ccgatgctgc cggaagtgct ttccaatttc
     2521 gtgtgctcgc tgcgacccaa tgaagaaaag ctgacgttct ccgctgtttt cgagctcgac
     2581 gaaaacgcaa aagttgtgtc ggaatggttc gggcgcacag ttattctttc cgaccgccgc
     2641 ttcacctacg aagaagtgca ggagatactc gaaggaaagc ccggcgatta tgcggaagag
     2701 ctccacgagc tcgacaaact ggcgaaaaaa ttacgcgatg accgcatgaa gaacgggtcg
     2761 atcaccttcg aacgcgaaga agtgaagttt cacctcgacg aaaacggcaa cccgaccggt
     2821 gtttatttca aagtgatgaa agactccaac cagctcatcg aggatttcat gctgctcgcg
     2881 aacaagcgcg ttgcacaata tggcgcactc gggcgcacga cgggagaagc gaagaaaaaa
     2941 cagggagatc tccggcggcc gtttgtttac cgcattcaca attctcccga cccggaacgc
     3001 gttgcggaat tcagcacatt cgtgaaaaat ttcggttacc acatgaatac gggaagcgag
     3061 aaggccgttt cgcaatcgct gaacaagctg ctgaaagacg tgcagggaaa accggaagcc
     3121 aacatggtgg aaacgctcgc gatccgcacg atggcaaaag ccatttattc cacggataac
     3181 atcgggcact acgggctcgg cttcgcttat tacacgcatt tcacttcgcc cattcgccgt
     3241 tatcccgatg tgatggcgca ccgtctcctg caatactaca tcgatctcga cgccggaaaa
     3301 aatgcggggc cggcacctga tgaaaacgag ctttcctcgc tgtgcaaaca ctcttccgac
     3361 cgcgaaaaat tagccgcgga agcagagcgt gcatcgatca aatacaagca ggtgcagttc
     3421 ctgcaagaca aggtcgggca agtgttcgat ggcattattt ccggcgttac cgaattcgga
     3481 tttttcgtgg aactggaagg aaacaaatgc gagggaatgg tgcacgtgcg caacctgaag
     3541 gacgaccggt atttcttcga agaagaaagc tactgccttc gcggacagcg ttacggaaaa
     3601 aaattcacgc tcggcgata
//
LOCUS       DASTSF010000254         5829 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1348456,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000254 DASTSF010000000
VERSION     DASTSF010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5829
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1348456"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..777
                     /locus_tag="VFU15_07365"
     CDS             <1..777
                     /locus_tag="VFU15_07365"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEU4717634.1"
                     /translation="YLRNTPAMLSDANRRRLHKYLSKVAFVPLHSRGELLRESMVFME
                     DLKLFPNQFAYIIDISNGTIPTHKGFDTFLGYADEKIDLEFLFGLFHPEDVEDLMNLI
                     TQAYDYGFSLEQPSPFCVQFMVTYRIRKADGSYIKVMRQSTLFQVDREHGMISTVSMC
                     SDITHIDGDKRIKFSMHGEEGKPFEVSDLRSKKTLQKFTRREAQLVSLISNGYSTKQI
                     AAELGTSVQTVETQRKNMLRKYNLANTFQLVFWAKENNLI"
     gene            complement(769..1566)
                     /locus_tag="VFU15_07370"
     CDS             complement(769..1566)
                     /locus_tag="VFU15_07370"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU4717635.1"
                     /translation="MTTSKNINGLDINYVETAGTGLPVFFIHGNSAGLRVFHRQLNGA
                     FGKRFRCIAFDMPGCGNSDRSADYSALFLARMIRDFILSFGFDSYAVVAHSLGGHLFF
                     ETQPAPGAKGVFLFGVPLLGASASTESPFLDNPVIPLLFSPDLSDADKALLIENFCAC
                     GSADDKKMLLSLIDRSDPGLRGGIGRSLNEGKINDEIAEFRRYDSIKQMMHCAGERII
                     NGASLEQFASFCAGGKILVIEGASHYPQLENPSAFENMLGDFLGKLN"
     gene            complement(1692..2075)
                     /locus_tag="VFU15_07375"
     CDS             complement(1692..2075)
                     /locus_tag="VFU15_07375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717636.1"
                     /translation="MKRILTPVNEKFIDERGILNIIIQEGAEITLENLREDHEMGLGL
                     TGGKKMRALVDARANFTSTEEAREFMRSDIMNKTRIATAVVTTNLASRIIVNFCLRFH
                     KPATPLKMFSDFDKAVKWLLSMKEH"
     gene            2347..4653
                     /locus_tag="VFU15_07380"
     CDS             2347..4653
                     /locus_tag="VFU15_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011711085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEU4717637.1"
                     /translation="MEQKPAVKPKAPQKSYRKFVVLFWIVVLAPVLGLLGTVSGIAMF
                     CDLPDIEQLQNPESNLATVVYSSDGKELGRYYAENRVNVQYKDIDPDVIHALVATEDE
                     RFYDHSGVDGRSLFRVLFKTVAGHDRSSGGGSTITQQLAKMQFHYEESQHATVSGRAL
                     QKLKEWIIAMRLERLYTKDEIITLYLNKFDFNYNAVGIKSAAKVYFGVGQDSLKVEEA
                     AMLVGMCQNPSKWNPVRFPDNAKKRRNIVLYQMMRNGYLTQVQFDSLKELPVKLHFSP
                     ESHNDGPAPYFREYLRDNFLRKWCSEHLKPDGKPYDMYRDGLKVYTTIDSRMQQYGED
                     AVAKHMKDLQAQFEVTLKNKRNRPFSWRVKKEEIDQIMKSAKERSDRYHHMKDGGATD
                     AEIDSAFVTKTPMTVFSWRGDIDTMMTPMDSIRYYKSFLQCGFMAMEPQTGYIRSWVG
                     GIDFKYFKFDHVKESKRQVGSTFKPFVYGMAVQEGFSPCDQVPCVRTCITTEDGKEWC
                     PSNAGGKADEKYEGKMITLRKALALSLNYISAYLMKQLGPQAVVQFAKNAGITSQLDP
                     NPSICLGTADISLYEMVGATSMFANKGTWIEPTFVTRIEDRNGKVLEDFVPKTREVMS
                     EEHAYIMLNMLQGVVDYGTGARLRSKYKLMNPIAGKTGTTQNNSDGWFMGLTPDLAAG
                     AWVGGEDRSVHFDLTDQGQGAAMALPIWAYFFQEVYADKSLKVSKGAFEKPKKKIQID
                     LDCSKLDAEDKDIFDDSNDSLFGTDDGS"
     gene            5072..5395
                     /locus_tag="VFU15_07385"
     CDS             5072..5395
                     /locus_tag="VFU15_07385"
                     /inference="COORDINATES: protein motif:HMM:NF016296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEU4717638.1"
                     /translation="MTFILVSGKTSVYPVQDKDIIRLQANGSYCIVFCKKGEQHVCSK
                     NLKSVLGTLPPALFVRVHQSHAVRISEIKFWEKGRGGSLTLKDGSVIPVSYRMKKQFT
                     DKLKK"
     gene            5392..>5829
                     /locus_tag="VFU15_07390"
     CDS             5392..>5829
                     /locus_tag="VFU15_07390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717639.1"
                     /translation="MTRLQRKWRSCKILVRVFENCIDFGRQKIVFCNDHIAIMNPSVT
                     FLGESAKFTANTGIAKVSTANSGLDGGGTLKKVLTAAGNGTYIATVTLKAPGNTGTSG
                     TIIRLFIDDGVNVRLLSEHLLGPTGGGTGSVFPTYCLVIPMNYT"
BASE COUNT         1529 a         1419 c         1544 g         1337 t
ORIGIN      
        1 tatttgagaa atacccccgc tatgctttcg gatgcgaacc gcagaagatt acacaagtac
       61 ctttccaaag ttgcattcgt tccgcttcat tcccgcgggg aattactccg ggagtcgatg
      121 gtattcatgg aagacctgaa gctttttcca aaccagtttg cctacatcat tgatatttcc
      181 aacggaacca tcccgactca caaagggttt gatactttcc tgggatacgc ggatgagaag
      241 atagacctgg aatttctttt cggattgttt cacccggaag atgtcgaaga ccttatgaat
      301 cttattacgc aggcgtatga ttatgggttt tcgctggaac agccttcccc gttctgcgtc
      361 cagtttatgg ttacttaccg catccgcaag gcggatggaa gttatatcaa ggtcatgcgg
      421 cagtcgacgc ttttccaggt ggacagggag cacgggatga tatccacggt cagcatgtgt
      481 tccgatatca cgcatatcga cggcgacaaa cgtattaagt tcagcatgca cggtgaagag
      541 ggaaagccgt tcgaagtgtc ggatctgcgc agcaaaaaaa cgcttcagaa attcacccgt
      601 cgtgaggccc agctggtatc gctcatttca aacggctatt ccacgaaaca gatagcagcc
      661 gagctgggca ccagcgtgca gaccgtggaa acgcagcgaa aaaacatgct gcggaaatac
      721 aatcttgcca atacgttcca gctggtgttc tgggcgaaag agaataacct aatttaattt
      781 cccgagaaaa tcgccaagca tattttcgaa tgcggacggg ttttcaagct gcgggtaatg
      841 agacgcgcct tcgatcacaa ggattttccc gccggcacag aacgatgcga actgctcaag
      901 gctggcgcca ttgatgattc gttcacctgc gcagtgcatc atttgtttaa tagaatcgta
      961 tcgccggaac tccgcgatct cgtcattgat ctttccttcg tttagcgacc ggccgatccc
     1021 gccccgcaat cccggatccg atctgtcgat caatgacagc agcatttttt tatcatccgc
     1081 agatccgcat gcacagaaat tttcgatgag gagcgccttg tcggcatcgc tgagatcggg
     1141 actgaacagc aacgggataa cggggttgtc gaggaaagga gattctgttg acgccgatgc
     1201 acccagcaaa ggaacgccga acaggaaaac gcctttcgct cccggcgcgg gttgtgtttc
     1261 aaaaaaaaga tggcccccga gcgaatgcgc gacgaccgca taggaatcga agccgaatga
     1321 aaggatgaaa tcgcggatca tgcgcgcaag gaaaagagcg gaataatccg ctgagcggtc
     1381 ggaatttccg cagcccggca tatcgaaagc gatgcagcgg aaacgttttc cgaatgcgcc
     1441 gttcagctgc cggtgaaaaa cacggagacc tgcagagttg ccgtgaataa aaaagaccgg
     1501 gaggcctgtt ccagcggttt caacatagtt tatatcaaga ccgttgatgt tcttcgaggt
     1561 tgtcatagtt tcataaaaat ccgaagatac gtaccagtag cttatcattg gcctgccggc
     1621 gtcgcggaaa acacgcattg caacgggaag aacaggaaaa ccggccgctc tgtaaacgaa
     1681 gcataaacgt ttcagtgttc cttcatcgac agcagccact tcaccgcttt gtcaaagtca
     1741 ctgaacattt tcagcggagt ggcgggcttg tgaaaccgga ggcagaaatt cacgataatg
     1801 cgggaggcga gattggtcgt caccaccgcc gtcgcgatcc gtgttttgtt catgatatcc
     1861 gaccgcatga attcccgcgc ttcttcggtc gacgtgaaat tcgcccgcgc atccacgagc
     1921 gcccgcattt tttttccgcc tgtcagcccg agccccatct cgtgatcttc acggagattt
     1981 tccagcgtga tctcggcgcc ttcctgtata atgatgttta aaatgccgcg ttcatctatg
     2041 aatttttcat tcacaggtgt caggatcctc ttcatggtgg gttttggaga aatactcccg
     2101 ttaaaataag aaaaggaggg gaaagcgcgc ccgtaaattg gttgcacagc ggaaggcagg
     2161 tttgaatttc tgtcaaaccc tgtgcttcac gtatgcgttt cccctgtccc ggaaatgtcg
     2221 cggagaatga gaaagcggtt ccgtgcggtg caccgggtgt tagggaaaga ccgttttttc
     2281 gaaaaaacac cacacttgtc cgcgtggttc cgtcccggtt ttgaatttga ctaattttga
     2341 tccgcaatgg aacagaaacc cgccgtcaag ccaaaagctc cccaaaaatc ctacaggaaa
     2401 ttcgttgttc ttttctggat cgttgtgctc gcgccggtcc tgggacttct cgggaccgtt
     2461 tccggcatcg ccatgttctg cgatctgccc gacatcgagc agctgcagaa cccggaaagc
     2521 aatctcgcca ccgtcgtgta ttcttccgat ggaaaagagc tcggacgtta ttacgccgag
     2581 aaccgcgtga atgtgcagta caaagacatc gatcccgacg tgatccatgc gctcgtggca
     2641 acggaagatg agcgcttcta cgatcattcc ggcgtggacg gaagaagtct tttccgcgtg
     2701 ctcttcaaaa cggtggcagg ccacgaccgc agttccggcg gcggctctac cattacgcag
     2761 cagctcgcga aaatgcagtt ccactacgaa gaatcgcagc acgccacggt ttccggaaga
     2821 gcgctccaga agctcaagga atggatcatc gccatgcgcc tcgaacgtct ttacacgaag
     2881 gacgagatca tcacgcttta cctgaacaaa ttcgatttca attacaatgc cgtgggaata
     2941 aaatcggcgg cgaaagtgta tttcggcgtt ggacaggatt ccctgaaagt ggaagaggcc
     3001 gccatgctcg tgggcatgtg ccagaatcct tcgaagtgga atcccgtgcg tttcccggat
     3061 aatgcgaaga agagaaggaa catcgtgctg taccagatga tgaggaacgg ttacctcacg
     3121 caggtgcagt tcgattcgct gaaagagctt ccggtcaaac tgcatttcag cccggaaagc
     3181 cacaacgacg gacctgctcc gtatttccgc gagtacctgc gtgataattt cctgcgcaaa
     3241 tggtgctcag agcacctcaa gccggacgga aaaccgtacg atatgtaccg cgacggactg
     3301 aaagtataca ccacgatcga ttcacgcatg cagcaatacg gcgaagatgc cgttgcgaag
     3361 cacatgaaag atctccaggc gcagttcgag gtgacgctga agaacaaacg caaccgcccg
     3421 ttcagctggc gcgtgaagaa ggaagagatc gaccagatca tgaaatcggc gaaggaacgt
     3481 tccgaccgtt atcatcacat gaaggacggg ggcgcgacgg atgcggagat cgacagcgca
     3541 ttcgtgacga aaacgccgat gacggttttt tcctggcgcg gcgatatcga tacgatgatg
     3601 acgcccatgg acagcatacg gtattacaaa tcattcctgc aatgcggctt catggcgatg
     3661 gaaccgcaga cgggctacat ccgttcgtgg gtgggcggta tcgatttcaa atatttcaag
     3721 ttcgaccacg tgaaagaatc gaagcggcag gtgggatcga cgttcaagcc gttcgtgtac
     3781 ggcatggccg tgcaggaagg attttcaccc tgcgaccagg tgccgtgcgt gcgtacgtgc
     3841 atcactaccg aagacgggaa agaatggtgc ccgagcaacg ccggcggaaa agccgatgag
     3901 aaatacgaag gaaaaatgat cacgctgcgc aaggcgctcg cgctttcgct gaattacatt
     3961 tccgcgtacc tgatgaaaca gctcggtccg caggccgtgg tgcagttcgc gaaaaacgcc
     4021 ggcatcacca gccagctcga cccgaatcct tcgatctgcc tcggaacagc ggacatttca
     4081 ctgtacgaga tggtcggcgc gacgagcatg ttcgcgaaca aaggcacgtg gatcgaaccg
     4141 actttcgtga cgcgcatcga agaccgtaac ggcaaagtgc tggaagattt cgttccgaaa
     4201 acgcgcgagg tgatgagcga agagcacgcg tacatcatgc tcaacatgct gcagggcgtt
     4261 gttgattacg gaaccggcgc gaggttgcgc agcaagtaca agctgatgaa tcccatcgcg
     4321 ggaaaaacgg gaaccacgca gaacaactcc gacgggtggt tcatgggcct cacgcccgat
     4381 ctcgccgcag gtgcgtgggt gggcggtgaa gaccgttccg tgcatttcga tctcaccgac
     4441 cagggacagg gcgcggccat ggcgttgccg atctgggcgt attttttcca ggaagtgtac
     4501 gccgataaat cgctgaaggt ctcgaagggc gcatttgaaa aaccgaagaa aaagatccag
     4561 atcgatctcg actgttcgaa gctggatgcg gaggacaagg atattttcga cgactcgaac
     4621 gattcgctgt tcgggacgga tgacgggagc tgatgagttg ggcgcgagat catctcacgc
     4681 ccaactccgg attttcagaa caccgttcgt tggcagcggt ttacagttat tggagatgac
     4741 cgggagaaaa agacaaacgg agacactatt gcaggtaaca gagatgaact tactatataa
     4801 catgttgtat attattttca cttaaaatat ttgaaattac attacagttt ttgttaattt
     4861 gtaaaggaga aattaagtga acgaattttt cagaatgcct ttgcccgatt ccccccgggc
     4921 tcaatttaat aggtcaaagg ttcttcagcg tcacgccccg atgctttggg acaatgagtg
     4981 tatagtcgtg aatttgtgag ggggaaatca aaaaaatcag ccgcggacgc agttatgcag
     5041 ttcgtgatag aaaccgtaca accagcccgg aatgacattt attcttgttt ccggaaaaac
     5101 gagtgtgtat cctgtgcagg acaaggatat cattcgcctg caggcgaatg gttcatattg
     5161 tattgtattc tgcaaaaagg gagagcaaca tgtatgctca aagaatctta agtccgtact
     5221 tggaacgttg cctcctgcgc ttttcgttcg tgtgcatcag agccatgcag tcagaatttc
     5281 tgaaataaag ttctgggaga aagggcgggg tggttcactc accctgaaag acggctccgt
     5341 gattccggtt tcataccgaa tgaaaaaaca gtttacggat aaactgaaaa aatgacgcga
     5401 ttacaaagga aatggcgaag ctgcaaaatt ctagtacgcg tatttgaaaa ttgcattgat
     5461 tttggcagac aaaagattgt tttttgtaac gatcatattg caattatgaa tccctcagtt
     5521 acatttcttg gcgaaagcgc aaaattcact gccaatacag gtattgcgaa agtaagtacc
     5581 gctaattcag gtctggacgg cggtggcaca ttgaagaaag tcctgacggc tgctggaaat
     5641 ggtacataca tagctactgt tactttaaaa gccccgggga atacaggaac aagtggtaca
     5701 attattcgtc ttttcattga tgatggcgtc aatgtaagat tactctccga gcatcttcta
     5761 gggccaactg gtggcggaac cggttccgtt tttcctacct attgtttggt cattcctatg
     5821 aactacacc
//
LOCUS       DASTSF010000255         7044 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_152829,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000255 DASTSF010000000
VERSION     DASTSF010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 7044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7044
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152829"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1938
                     /locus_tag="VFU15_07395"
     CDS             <1..1938
                     /locus_tag="VFU15_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEU4717640.1"
                     /translation="FSINVLTLLGIVLATGLVVDDGIVVTENIYKKIEKGMDKRTAAR
                     EGTKEIFFAVISTSATLAVVFIPVIFLQGFTGRLFREFGIVVAGSVLISALVSLTLTP
                     VLNVLLSRKTQKKSRFYEWSEPFFHGMEMRYRRSLEKFMKRRWLGIAVLGICLGLIFL
                     SVRFLNSELAPLEDRSVVRCPVSAPEGTSFDYTDDIIYRFTQNTIDSFPDAHMIFSIV
                     APPFGTSSSTNSGMVIVRLSDPEDRNYTQQQVADKLNKMGKNFPEAKIFANQEQTIST
                     SLSSGSQLPVQLILQNLDFEKLRSVVPKIMDDAQKDSVFSNVDVNLKFNKPELDVTID
                     RLKAAQLGVSILDISNTLQLALSGKRFGYYQVNGKQYQVIGQVDRENRDDPADLSSLF
                     VKSRSGEMVQLSNLVKTEENINPPTLYHYNRFKAATISAGLAPGKTLGEGIAEIRRIA
                     AKYTDSSFQTTLGGASRDLTESSSNTSFAFILALVLIYLILAAQFESFRDPLTIMLTV
                     PLAIAGALCSLWLFGQTLNIFSQIGMIMLIGLVTKNGILIVEFSNQLREKGQLKMEAV
                     KNSAAARLRPILMTTLATVLGAMPIALALGAGAKSRVPLGVVIVGGLLFSLLLTLYVI
                     PAMYSYIAAGDNYAPRKQPEE"
     gene            1935..3227
                     /locus_tag="VFU15_07400"
     CDS             1935..3227
                     /locus_tag="VFU15_07400"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEU4717641.1"
                     /translation="MKKTFLFLLTVSGSLLCHAQVMTPDDALQAGLKNNFDVIIAKNA
                     AEADSIMNSPGEAGMLPTVSLNGSFGVNQNSIHQKYANGNEFTANNAGSNSLTSSVSL
                     SWTLFDGTKMFVTKHKLETLQMQGEYEFRSQVLNTSASILTSYYDIVRQKQKLNAIDE
                     VIKANQQRLDITETRFTSGSGPKTDYNQAKIDLNVQLENRLVQAEALSESKRTLNNLL
                     ARDVNTSFDVIDSIPEGALPDRQQLEQKMYASNPDLLAFKAQVDVTKFEVREARSQYF
                     PRLGVQAGYSFSQNQNSAGFSLYNQSNGPTAGVTLAFPIYQAGKVKRQVAVSTLDVNS
                     AEMQFARANLEASLQLQNGLSTYDLKAQSIVLEKENVSLARENMQMALDRLNLGQGTA
                     FEVQQAQITLSSSLSRLSDLQYEMKAADISVHRIAADL"
     gene            3303..4616
                     /locus_tag="VFU15_07405"
     CDS             3303..4616
                     /locus_tag="VFU15_07405"
                     /inference="COORDINATES: protein motif:HMM:NF038133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEU4717642.1"
                     /translation="MHRALFFFFLIPALVHGQITFESKQSVEFYVKNVLMGPGVYVAN
                     VKHYGMIGGMGQFEADSSILGVKSGLVLSTGVVDSLHGPNNSSGYTSFGKFPADAQEQ
                     KKVLRGDRNMNVICKGHAVDMTVIEFDFVPVNNVLEFNYVFGSEEYPEYAKSKYDDGF
                     GFFLRGPGIHGTKNLAVLPNGKTPVNVNSINAKKHSKYFRKNYGKINRMSARSKKSFY
                     RSKKKALRVLLMKSIQLDGLTTVLKVHADLIPYQLYHMKIGIADASDELFDSAVFLQA
                     GSFVSIADTTAKYALALSELKDDHPDPDSVLKGLKRPPPVKNIVAPPTDDEKFSMTNI
                     YFDTDKNVIPDSSKKELDALVNYLGTHDTLVCHITGYADNSGNVNHNKKLSEKRAQAI
                     RDYLVAHGIKPGKIIVEGYGIDDPDADNSTAHGRALNRRVEISLE"
     gene            4727..5257
                     /locus_tag="VFU15_07410"
     CDS             4727..5257
                     /locus_tag="VFU15_07410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717643.1"
                     /translation="MRKRFLLPAVCALLVTCTTYDNAIIYHKNFTPGTNALLRFNGFY
                     SDTLGPVEAKSAKQVTRKIKPVFFYADGSAFSTRDYQPSSSAGLTSATGFWGNYRIIG
                     DTILLEKFEEAEASSNLARIILKGVVHEGSIEWMARKDRNSDYQPVKYSIYFTPFAAK
                     PDSTQNWIRTKKKYNK"
     gene            complement(5330..5686)
                     /locus_tag="VFU15_07415"
     CDS             complement(5330..5686)
                     /locus_tag="VFU15_07415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717644.1"
                     /translation="MEQTNAPSTKGKGLGMAGMIIGIVGLVWAIIPLLGAGAIWLALP
                     GLILSVVAFFMAKNGNNPKKGAIITGLILNLVALIIAAYWIYAIAKTATEIGSKIQNI
                     DTAQLRKDLENSMNGN"
     gene            complement(5821..>7044)
                     /locus_tag="VFU15_07420"
     CDS             complement(5821..>7044)
                     /locus_tag="VFU15_07420"
                     /inference="COORDINATES: protein motif:HMM:NF017142.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEU4717645.1"
                     /translation="SLKHNVFVLDGVEFHLWFQGECQPEWPKLISDFFIFINEQMVIF
                     GSFPSTEYHFLFQVLPNRFYHGVEHRDCTVIALGPSYKLMNEVYGDLLGVSCHELFHA
                     WNIKSIRPAEMYPYDLTKENYSRLGYVCEGVTTYYGDYLLFRSGVWNEEEYWPTFIER
                     MEKHFESHARYFLSVADSSFDTWLDGYVPGAPHRKTSIYHEGCLLAFVTDIFIRRHSK
                     NAKSLDNVMRRLWNEFACKDKGYTGADYKKLVEEEAGADFSYYWNNYFYKGNSYEPVL
                     SEALAYIGYELTFEPSKRVHERDYGFKVAEPGGLTKVTEVYYGSPAEKCGLAPGDEVL
                     AVNGMQLKADMAEWLKYFGGEEAELIVASSGRVKTVVLRTDGKHYYDRPGVKRKADAD
                     GPARESFSAWSGKKF"
BASE COUNT         1713 a         1900 c         1827 g         1604 t
ORIGIN      
        1 ttttcgatca acgtgctcac gctgctcggg atcgtgctgg ccacgggact tgtcgtggat
       61 gacgggatcg tggtgacgga gaacatttac aagaagatcg aaaaaggcat ggacaagcgg
      121 actgcggcgc gggaaggaac gaaagagatc ttcttcgcgg tcatctccac ttccgccacg
      181 ctggctgtcg tttttattcc cgtcattttt ctccagggtt ttaccggccg gctgttccgt
      241 gaattcggga tcgtcgttgc aggatcggtg ctgatctccg cgcttgtttc gcttacgctt
      301 acgcctgtgc tcaacgtcct cctcagccgg aagacgcaga aaaaatcgcg tttctacgaa
      361 tggtcggaac cttttttcca tggaatggaa atgcgttacc gccggtcgct ggagaaattc
      421 atgaagcgca ggtggctggg tatcgcggtg ctcgggatct gtctcgggct cattttcctt
      481 tcggtaaggt tcctgaattc ggagctggcg ccgctggaag acaggagcgt cgtgcgttgc
      541 ccggtttccg cgccggaagg aacttcgttc gattacaccg acgatatcat ttaccgtttt
      601 acgcagaaca cgatcgacag ctttcccgat gcgcacatga tcttcagcat tgttgcaccg
      661 cctttcggca cgtccagcag tacgaattca ggaatggtga tcgtgcgcct gagcgacccg
      721 gaagaccgga actacacgca gcagcaggtg gcggacaagc tcaataaaat gggcaagaat
      781 tttcccgaag caaaaatttt cgcgaaccag gaacagacga tcagcacttc actctcttca
      841 ggctcgcaat tgcccgtgca gctcattctc cagaacctcg attttgaaaa gctgcgcagc
      901 gttgttccga aaataatgga cgatgcgcag aaagattccg ttttttccaa cgtagatgtc
      961 aacctgaaat tcaacaagcc ggaactcgac gtgacgatcg accggctgaa agcagcgcag
     1021 ctcggtgtca gcatcctgga tatttccaac accctgcagc tggcgctttc gggaaaacgc
     1081 ttcggctatt accaggtgaa cggaaagcaa taccaggtga tcgggcaggt cgaccgagag
     1141 aaccgcgacg acccggccga cctttcttcg cttttcgtga aaagccgcag cggtgaaatg
     1201 gtgcagcttt ccaatctcgt gaaaacggaa gagaacatca acccgccgac gttgtatcat
     1261 tacaaccggt tcaaggccgc gacgatctcc gccgggctcg caccgggaaa aacgctgggc
     1321 gaaggcattg ccgagatccg gaggatcgcc gcgaaataca cagacagcag tttccagacg
     1381 actttgggcg gtgcatcgcg tgatctcacg gaaagctcgt cgaacacttc gttcgcgttc
     1441 atacttgcgc ttgttctcat ttacctgatc ctggccgcgc agttcgaaag cttccgtgat
     1501 ccgctgacga tcatgctcac cgttccgctc gccattgccg gcgcgttatg ctcgctgtgg
     1561 ctgttcgggc agacgctgaa tattttctcg cagatcggca tgatcatgct catcgggctg
     1621 gtgacgaaaa acgggattct catcgttgaa ttttccaacc agctgcgcga gaaagggcag
     1681 ctgaaaatgg aggccgtgaa gaacagcgcc gcagcgaggc tccgccctat cctgatgacg
     1741 acgctcgcca ccgtgctggg cgccatgccg atcgcgcttg cgctcggtgc aggagcgaaa
     1801 agccgcgtcc cgctgggcgt cgtgatcgtc ggcggacttt tattctcact gctgctgacg
     1861 ctctacgtta ttcccgccat gtacagctat attgccgcgg gcgataatta tgcaccgcga
     1921 aaacaaccgg aagaatgaaa aaaacatttt tatttctgct gaccgtttcc ggtagcctgc
     1981 tgtgccacgc gcaggtgatg acgcccgacg atgcgttgca ggccgggttg aaaaataatt
     2041 tcgacgtgat cattgcgaaa aatgccgcgg aggccgattc tatcatgaac agtccgggcg
     2101 aggcgggcat gctgcccacg gtgagcctca acggcagctt cggtgttaac cagaacagca
     2161 tccaccagaa atatgccaac gggaatgaat tcaccgccaa caatgccgga tcgaattcgc
     2221 tgacgtcttc cgtttcgctt tcctggacgc ttttcgacgg aacgaaaatg ttcgtcacaa
     2281 agcacaagct cgaaacactg cagatgcagg gcgaatatga gttccgttcc caggtgctca
     2341 acacgagcgc atccattctc acgtcctact acgacattgt ccggcagaag cagaagctga
     2401 acgcgatcga tgaagtgatc aaagcgaacc agcagcgcct cgatatcacc gaaacccgtt
     2461 ttacttccgg ttccgggccg aaaacggatt acaaccaggc gaagatcgat ctcaacgtcc
     2521 agctcgagaa caggctcgtg caggcggaag cgctcagcga atcgaaacgc acgctgaata
     2581 atttgctggc ccgcgatgta aacacttcat tcgatgtcat cgacagcatt cccgagggcg
     2641 ctcttcccga ccggcagcag ctggagcaga aaatgtatgc gtcgaatccc gacctgcttg
     2701 cgttcaaagc acaggtagac gtgacgaagt tcgaagtgcg tgaagcgcgt tcgcaatatt
     2761 ttccccggct cggcgtgcag gcgggatatt cgttcagcca gaaccagaat tcagccggct
     2821 tcagcctgta caaccagtcg aacgggccga ccgcaggtgt aacgctcgct ttccccattt
     2881 accaggcagg gaaagtaaaa aggcaggtcg ctgtgtcgac gctcgatgtg aactccgctg
     2941 aaatgcaatt cgccagggca aacctcgaag ccagcctgca actgcagaac gggctttcca
     3001 cgtacgacct caaggcgcag tcgatcgtgc tggaaaaaga gaacgtctcg ctcgcccgcg
     3061 agaacatgca gatggcgctc gaccgtctca atctcgggca gggaacggca ttcgaagtgc
     3121 agcaggcgca gatcacgctt tcttcttccc tttcccgtct ttccgacctg cagtacgaaa
     3181 tgaaagcggc ggatatttcc gttcaccgca ttgccgcaga tctctgagcg accgcttttc
     3241 gggcaaatta acccgctact tttgtgtaac cacccccttt ttccgcgtta tacaactgga
     3301 aaatgcacag agcactcttt tttttctttt tgatcccggc acttgttcat ggacagatca
     3361 cgttcgagag caaacagagc gtggagtttt acgtgaagaa cgtgctcatg ggccccggcg
     3421 tttacgtggc gaatgtgaag cactacggga tgatcggcgg gatgggacaa ttcgaagccg
     3481 attcgtccat tctcggggtg aagtcgggac ttgtgctttc caccggcgtt gtcgacagtc
     3541 tgcacggccc gaacaattcc agcgggtaca cttccttcgg aaaattcccc gcggatgcac
     3601 aggagcagaa gaaagtgctg cgcggcgacc gcaacatgaa cgtcatctgc aaaggacatg
     3661 cggtggacat gaccgtcatc gaatttgatt ttgtgccggt gaacaatgtg ctcgaattca
     3721 attacgtttt cggttccgaa gaatatcccg aatatgccaa aagcaagtac gacgacggct
     3781 tcggattttt cctgcgcggc ccggggatcc acggaacgaa aaacctggct gtgctgccga
     3841 acggaaagac tcccgtgaat gtgaattcca tcaatgcgaa aaaacattcg aagtatttcc
     3901 ggaagaatta cggcaagatc aaccgcatga gcgcacgttc gaaaaaatct ttctaccgca
     3961 gcaaaaagaa agccctgcgc gtgctgctca tgaaatccat acagctcgac gggctcacca
     4021 ccgttctgaa agttcacgcc gaccttattc cctaccagct ttaccacatg aagatcggca
     4081 tagcggatgc gtcggacgaa ttattcgact ccgccgtttt cctccaggcc gggtctttcg
     4141 tcagcattgc ggatacgaca gccaaatatg cgctggcgct gagcgagctg aaagatgatc
     4201 atcccgatcc cgacagcgtg ctcaaaggac tgaagcggcc gcccccggtg aaaaatatcg
     4261 tggcaccacc gacggatgac gaaaagttca gcatgaccaa tatttatttc gacacggaca
     4321 aaaacgtcat tcccgacagc agtaaaaaag agctcgatgc gctcgtgaat tacctgggaa
     4381 cacatgacac gctcgtgtgc cacatcaccg ggtacgccga caactccggg aatgtaaacc
     4441 acaacaagaa gctgtccgaa aaaagagcgc aggcgatacg cgattacctg gtggcgcacg
     4501 ggatcaaacc cggtaagatc attgtggaag gatacggcat tgatgatccc gatgcagaca
     4561 attccactgc gcacgggcgc gcgcttaacc gccgggtgga gatctcgctc gaatagttca
     4621 ttgcgttcgg tttccttttt ctgtccggca attttactgt tgtgaaccca aatgcaggtt
     4681 ttcattccgg gagcgggctg ctgccgaaaa ctgactaagt ttggaaatgc gaaaacgttt
     4741 tcttctgccc gcggtctgcg ccctgctcgt cacgtgcacg acgtacgaca atgcgatcat
     4801 ttaccacaag aattttacgc cgggaacaaa tgcgctgctg cgtttcaacg ggttctattc
     4861 cgatacgctc ggtcctgtgg aagcgaaaag cgcgaaacag gtaacgcgga aaataaaacc
     4921 ggttttcttt tatgcggacg gttccgcgtt cagcacacgc gattaccagc catcctcatc
     4981 cgccgggctg acttcggcaa ccggtttctg gggaaattac aggatcatcg gcgatacgat
     5041 cctgctcgag aaattcgaag aagcggaagc ctcgtccaac ctcgcgcgca tcatcctgaa
     5101 aggcgttgtt cacgaaggaa gcattgaatg gatggcgcgg aaggacagga actcggatta
     5161 ccagccggtg aagtattcca tttacttcac gccattcgca gcaaagccgg attcgacgca
     5221 gaactggatc aggacgaaga agaagtacaa taagtgaaga aacgaagctg attttaccca
     5281 ataaaaaagc cacccggagg tggctctttt attttctgat gaacgaaaat cagtttccgt
     5341 tcatgctgtt ctcaagatct ttacggagct gggccgtatc gatattctga attttgcttc
     5401 cgatctccgt tgctgttttc gcgatggcat agatccagta agccgcgata atcaatgcaa
     5461 ccaggttaag gatgagtccc gtgataattg cgcctttctt cgggttattg ccattcttgg
     5521 ccatgaagaa agcgacgaca ctcaggatga ggccgggaag tgcaagccat attgcacccg
     5581 cgccgagcaa aggaatgatt gcccatacca ggccaacgat gccgatgatc ataccggcca
     5641 ttccgagacc tttacccttg gtagagggag cgtttgtttg ttccatagta gttttggttt
     5701 ggtttagtgt tgatttgtct gagagcaaaa caataacatt tcgaccagtt ttcaaacaag
     5761 gtcacattca atttattaac actttttagt tgctgtttgt cagtgattta aagcggtttt
     5821 tcagaacttt ttaccgctcc acgccgaaaa agattccctc gcagggccgt ctgcatccgc
     5881 ttttcttttc accccgggac gatcatagta atgcttcccg tccgttcgga gaacgaccgt
     5941 tttgaccctt ccggaagaag cgacgataag ctccgcttcc tctcccccga aatatttcag
     6001 ccattccgcc atgtccgctt tcagctgcat cccgtttacg gcgagcactt catcgcccgg
     6061 agccagcccg catttttctg ccggcgaacc gtaataaact tccgtgactt tcgtgagtcc
     6121 gcccggttcc gccactttga aaccgtaatc ccgttcatgt acgcgcttcg acggttcgaa
     6181 tgtcagctcg taaccgatgt acgccagcgc ttccgacagg acaggctcat agctgttgcc
     6241 tttgtagaaa taattattcc agtaataaga aaaatcggcg ccggcttctt cttccaccag
     6301 ctttttataa tcggccccgg tgtagccttt gtccttgcat gcaaattcat tccacagcct
     6361 tcgcataacg ttgtcgagcg actttgcatt tttcgaatgg cggcgaatga aaatatccgt
     6421 cacgaaagcg agcaggcatc cttcatggta gatcgatgtt ttccggtgcg gcgctcccgg
     6481 aacataaccg tcgagccacg tgtcgaagga agaatcggcg acggaaagga aatagcgcgc
     6541 gtgcgattcg aaatgctttt ccatccgctc gatgaacgtg ggccagtatt cttcttcatt
     6601 ccatacgccg gaacggaaca gcaggtaatc gccgtaatag gttgtcacgc cttcgcacac
     6661 atacccgaga cgcgaataat tttctttcgt caggtcgtac ggatacattt ccgccgggcg
     6721 gatcgatttg atgttccagg cgtgaaaaag ctcgtggcag ctcacaccga gcaggtcgcc
     6781 gtacacttcg ttcatcaatt tgtacgaagg tccgagcgcg atcaccgtgc agtcgcgatg
     6841 ctccacgccg tggtaaaagc ggttcggcaa tacctggaaa agaaaatggt attcggtgga
     6901 aggaaaactt ccgaagatca ccatctgctc attgatgaaa atgaaaaaat cggaaatgag
     6961 cttcggccat tcgggctggc attcaccctg gaaccagagg tggaattcaa cgccgtcgag
     7021 cacgaaaacg ttatgcttga gcga
//
LOCUS       DASTSF010000256         8789 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1583140,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000256 DASTSF010000000
VERSION     DASTSF010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 8789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8789
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1583140"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(204..893)
                     /locus_tag="VFU15_07425"
     CDS             complement(204..893)
                     /locus_tag="VFU15_07425"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU4717646.1"
                     /translation="MKINCIIVEDEPPAAKLMEDNVQRVSFLELCGIFPGAAQAEAFL
                     ATRTIDLVFLDIRMPGRSGISLSKMLPKETMVIFTTAYENHALEGFEVNAVDYLLKPF
                     SFERFEQACRKAKELKELRASQAPKKIQVRSSHSIVYLDPCEITHVEGLKDYVKIFVA
                     GNPKPVLSRMNLKGAETILPPAFIRVHKSYIVNSSLITKTGKDLVHLGDILVPVGDAF
                     RQALAEKTGTR"
     gene            complement(868..1896)
                     /locus_tag="VFU15_07430"
     CDS             complement(868..1896)
                     /locus_tag="VFU15_07430"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEU4717647.1"
                     /translation="MKRKRLSVSLFVVQALAWTLWIALPYLNPDPGIREYHLPYHIKH
                     FSEHLMYLVFYYVNYYFLVPKFLPEKGTTKYALTVTGIMLFIFTVNNFVEYAVFGNYR
                     IQKPVSFVSLVPMMSVYILSTTLSLFENWFRDKNEKKKLTQEKENAEIGLLRMQMSPH
                     FLFNSLNNISSLVRFDQENAEKYIQQLSGLMRYTLNVSGEKKVPLQSEIGFLENYISL
                     QKLRLPENFDLRTRFPSHVNGASVEPLILVSFVENAFKHGNNGNAADFIEMNLDVNGN
                     RLVMETKNLVSAGTDKDLSTGIGLKNVRKRLAIVYPGRHVLIAEEKDNVFNVHLEIEL
                     NEDQLHHR"
     gene            2032..2637
                     /locus_tag="VFU15_07435"
     CDS             2032..2637
                     /locus_tag="VFU15_07435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717648.1"
                     /translation="MKRTLLLNFCLFISVCAFAQDNGAAGDKGRITSISFGLNRATSV
                     LFRGIFGREEPYVYNFSAQFPARKSDAYCWNFGIDFGDRVYPGFWDRDFFLGLEGGIQ
                     HPFLSPDSPWILNGQVSLFLGRYSEDYSFDRSQLYFNTIGVTPEIQLGKKLNDKLSIF
                     TSFSTEIGYFDWGGQESGHTAPFAFYPYRFLSVQVKYNLAE"
     gene            complement(2643..3326)
                     /gene="tsaB"
                     /locus_tag="VFU15_07440"
     CDS             complement(2643..3326)
                     /gene="tsaB"
                     /locus_tag="VFU15_07440"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013632805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEU4717649.1"
                     /translation="MILLIESSAATCSVALSARGEIVSIREIREPNKHAEVLTVFCDE
                     VMKDAGLSFAQLDAVAVSKGPGSYTGLRIGVSAAKGICYATGKPLIAVGTLDAMAQGM
                     KAEAKANDLLCPMIDARRMEVYCALFDADANEISPVEPKVIDENSFAEILAGQKILFS
                     GDANTKVKDVLGRFPNAVFTETGMHSAKHLALPAEKKFAAKDFEDLAYFEPFYLKTFH
                     PGPQRSKTE"
     gene            complement(3362..4732)
                     /locus_tag="VFU15_07445"
     CDS             complement(3362..4732)
                     /locus_tag="VFU15_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013667383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEU4717650.1"
                     /translation="MKRILIIGGIIVAVLITVVMLTKGSGKGTERVATEKAQKRNITE
                     VVSASGKVQPEVEVKISSDVSGEIVELDVKEGDTVKKGDLLLRINPVIYESTVNEMQA
                     SVDGAHANLANSRARLDQSKANLNNAEASFTRNKKLHDQGAISDAEFDQANATYKSAL
                     ADVDAATQSVHSAESNVSNAEASLKQAQDNLAKTNIYAPVTGTVSVLNKKKGERVVGT
                     AQMEGTEIMRLANLDEMEVQVDVNENDILRVKEGDTAMIEVDAHNNRKFKGVVTEVAN
                     SANTTGLTTDQVTNFQVKIRILRDSYSDLIDPQHPDRYVFLPGMSASVEIMTNSVRDV
                     ISVPIISVTTRTDTASPNAVAKKEEKPSGGDENQDIVVHDTKDNTKTAETKSIECVFV
                     YADGKVKLIPVVTGVEDNMYIQIISGIKEGDEVVSAPFRAISTSLYNGAKVEKVSKES
                     LSTIED"
     gene            complement(4745..6262)
                     /locus_tag="VFU15_07450"
     CDS             complement(4745..6262)
                     /locus_tag="VFU15_07450"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEU4717651.1"
                     /translation="MKRILLTPVLLLFAVLTGLTASGQTFVKMDPAADTSLLRVVAAN
                     SDSALFPADNSQPWNLTQCIAYAQVHNITVQQQLLNVQLAQVNLRQSQGNMLPNVNGF
                     ASHTYQYGRTVDRFTNTFANSMVLSENFYLSSNWTVFNGMQNFNTMKQDQYALQGSRY
                     QVQQTQDDIGMNVASAYLNVLYAQEALGVSQQQVDLTQAQVDRTQKLVDAGAAAKGSL
                     LDLQSQLATEQVSLVTAQNNLTIAYLTLTQMMNLDSTSGFTIVKPIIDVPNESLLNTT
                     PDLIFQAAMNNQAGIKKSQMDVKSADKGVDVAYGGLSPTVTIQGSIGTGYSGAAKTVT
                     GTSYSGYDTAGVTTNGGYVLIPNYTTTYITTPFSDQFNNNVNKSFGVQVTVPIFNRFQ
                     VMDNVDRAKINYENAQLSLDLSQQQLMKNIQQAYADAQAALQKYQASQKAVDAAQESF
                     NYTEQKFDVGAVNSIDYNNAKNKLTKAQSDLIQAKYDYIFRMKVLDYYQGKPLTF"
     gene            6329..6907
                     /locus_tag="VFU15_07455"
     CDS             6329..6907
                     /locus_tag="VFU15_07455"
                     /inference="COORDINATES: protein motif:HMM:NF016239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HEU4717652.1"
                     /translation="MNFLAHFYLSGTNENLLSGNFIADAVKGSDLSAFPEGIREGIIM
                     HRQIDHFTDRHPVVMKSIKRLRPDFHHYSPVITDIFFDHFLATNWNEYSEEPLAGYAA
                     RTYAILEKYVDVFPEKSKRILPFMKQHDWLTIYSRVEGVHNVLTGMSRRTTFDSGMER
                     AAEALVRDYDAFGDDFRKFFPDIIAHTQKFRS"
     gene            complement(7066..7728)
                     /locus_tag="VFU15_07460"
     CDS             complement(7066..7728)
                     /locus_tag="VFU15_07460"
                     /inference="COORDINATES: protein motif:HMM:NF025718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2-related protein"
                     /protein_id="HEU4717653.1"
                     /translation="MKITSHGLKQTTWEKIRADASFRKQLLISFLFLAVVLAGFFFVL
                     AFVENRDGLIIEKGWLDGIASTHDFSAIIFPLTYSTIIAGVIIAAQNPEKLLLMIRAY
                     AFLQLLRGISLLVFPLDPPGGIIPLHDPFLQSTFYNGRANLKDLFFSGHVATIVMFIP
                     VLGRSWLKYTFALSALAVGILLVVQRAHYVADVAAAPLFSGLSFWLASSWTSSSSKES
                     AG"
     gene            complement(7766..8485)
                     /locus_tag="VFU15_07465"
     CDS             complement(7766..8485)
                     /locus_tag="VFU15_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009185057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEU4717654.1"
                     /translation="MKAVIIDDERLARQELKSMLSTYPGVEIAGECADVKSAAETINN
                     VKPDVIFLDIQMPGKNGFDLLEEITFVPEVVFVTAHDEFAIRAFEVNALDYLLKPVQP
                     ARLSDAIRKINSHLNEKKEAKDTGNTLGEDDQVFVKDGERCWFVKLGDVRLFESEGNY
                     VRLYFGTNKPLILRSLNYLDERLNNKAFFRASRKHIINLRWVENIESWFNGGLLVKLR
                     GGEQVEISRRQAVKLKEMMSL"
     gene            complement(8482..>8789)
                     /locus_tag="VFU15_07470"
     CDS             complement(8482..>8789)
                     /locus_tag="VFU15_07470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEU4717655.1"
                     /translation="CEVPPLMVQTLVENGIKHGIARLPGGGKLEIASQNNDSGTLITI
                     RNSGTYDAEKKSESGFGMKNTLERLALLYGDTAKFSIANENNGTVITELFIPKSYNA"
BASE COUNT         1879 a         2290 c         2400 g         2220 t
ORIGIN      
        1 gcgccgatcg tatcgttggt atttggtgta attgtatagg tggatacggg cgtgtgcgta
       61 acctgtgcgc aaaggatctc cgaaaggatc atcccggcga cgatgaggaa ttttttcata
      121 gaaaataaat ttgcagcaat ttaaccggat acgaattccg tcatggaaat tgtagacgaa
      181 ctgcccggat ttgtatagga atgttaccgt gtgccggttt tttccgccag cgcctggcgg
      241 aacgcgtcac cgacgggaac caggatatca ccgaggtgaa cgaggtcttt tcccgttttc
      301 gtgatgagag acgaattcac gatgtacgat ttatgcacac ggatgaacgc gggcggcagg
      361 attgtttccg cacctttcag gttcatccgg gaaagtacgg gcttcgggtt cccggcgacg
      421 aagatcttga cgtaatcctt cagcccttcc acatgtgtga tctcgcacgg atcgaggtaa
      481 acgatggaat gcgaggagcg cacctgaatt ttcttcgggg cctgggaggc gcgaagttct
      541 ttcagctctt tcgctttccg gcaggcctgc tcaaaacgtt cgaaggagaa tggcttgagc
      601 aggtaatcga ccgcgttcac ctcgaatcct tccagtgcgt ggttttcgta agcggtggtg
      661 aagatcacca tcgtttcctt cggcagcatc ttcgaaagcg aaatgccgga acggccgggc
      721 atgcggatgt cgaggaaaac gagatcgatg gtccgggtgg cgagaaaagc ctccgcctgc
      781 gccgcgccgg gaaaaatccc gcagagttcg agaaaggaaa cgcgctgcac attgtcctcc
      841 atcagcttcg cggcaggcgg ttcgtcttca acgatgatgc agttgatctt cattcagttc
      901 gatttcaaga tgaacgttga atacattgtc cttttcttcc gcaatgagca cgtgtcttcc
      961 cgggtaaacg atcgccaggc gtttgcgtac gttcttcagc ccgatgccgg tggaaagatc
     1021 cttgtccgtt cccgctgaaa caagattctt cgtttccatc acgagacggt ttccgttcac
     1081 gtcgaggttc atttcgataa aatcggctgc attcccgttg ttcccgtgct tgaacgcatt
     1141 ttccacgaag ctgacgagaa tgaggggctc gacggaagcg ccgttcacat gggaaggaaa
     1201 gcgggtgcgc agatcgaaat tttccgggag acggagcttc tgcaggctga tgtaattttc
     1261 aagaaaaccg atctcgctct gcagcggaac tttcttttca cctgaaacgt tcagcgtgta
     1321 gcgcatgagt ccggaaagct gctgtatgta tttttctgca ttctcctggt cgaaacgcac
     1381 gagcgatgaa atgttgttca ggctgttgaa caggaaatgc gggctcatct gcatgcgcag
     1441 cagcccgatc tctgcgttct ctttttcctg cgtgagcttt ttcttttcgt tcttgtcgcg
     1501 gaaccagttc tcaaaaagcg agagcgttgt gctgagaatg taaacggaca tcatcggtac
     1561 cagcgaaacg aacgaaacgg gtttctgtat gcggtagttg ccgaacacag cgtattccac
     1621 gaaattgttc actgtaaaaa tgaacagcat gatccctgtt accgtgagcg cgtatttcgt
     1681 cgtgcctttt tccgggagga atttcggaac gaggaaataa taattcacat aatagaaaac
     1741 gaggtacatc aggtgttccg aaaaatgctt gatgtggtac ggaagatgat attcccggat
     1801 gccggggtcg ggattgagat acggcagcgc gatccagagc gtccaggcca gcgcctgcac
     1861 gacaaacagc gaaacggaaa gccgctttct tttcatcggt ggctgaaaat tactttttta
     1921 tctttcaaac ggcaggggaa tgtagagcaa catcacctgt ttgtagagca atcgtcataa
     1981 gatgcccgca aatacggcag gaaatttgga caaaacccga aaatacgaac gatgaaacga
     2041 accctgcttc tgaacttttg cctgttcatt tctgtgtgcg cctttgcgca ggataacggc
     2101 gcagccggtg acaaaggaag gatcaccagc atctctttcg gactgaaccg cgcaacatcg
     2161 gttctgttcc gcggaatttt cggaagggaa gagccctatg tgtataattt ttccgcccag
     2221 tttcctgcac gaaaatccga cgcgtactgc tggaatttcg ggatcgattt cggagaccgc
     2281 gtttatcccg gcttctggga ccgcgatttt ttcctgggac tcgaaggcgg tattcagcac
     2341 ccgttccttt cacccgattc tccctggatc ctcaacgggc aggtgtcgct tttcctcggg
     2401 cgttattccg aagattattc cttcgaccgc tcgcagcttt atttcaatac gatcggtgta
     2461 acgcccgaaa tccagctggg aaaaaagctt aacgacaagc tcagcatttt tacgtcgttc
     2521 agcacggaga tcggttattt cgactggggc ggacaggaaa gcgggcacac agcgccgttc
     2581 gcgttttatc cttaccgttt tctttccgtg caggtgaaat acaatcttgc ggaataaagt
     2641 agttattccg ttttcgagcg ttgcgggccg gggtggaacg tcttcaggta aaaaggttcg
     2701 aaataggcga ggtcttcgaa atcttttgcg gcgaattttt tttccgcggg aagagccagg
     2761 tgctttgcag aatgcattcc tgtttccgtg aaaaccgcat tcggaaaacg accgagaacg
     2821 tccttcactt ttgtatttgc atcgccggaa aaaaggattt tttgcccggc gagaatttcc
     2881 gcgaagctgt tttcgtcgat gaccttcggc tcgacgggtg aaatttcatt cgcgtccgca
     2941 tcgaacagcg cgcaatacac ttccatccgc cgcgcgtcga tcatcgggca gagcaggtcg
     3001 ttcgctttcg cttctgcttt cattccctgt gccatcgcat ccagcgttcc cactgcgatg
     3061 agcggtttcc ccgttgcgta gcaaattcct ttcgccgcgg aaacaccgat gcgcaggccg
     3121 gtgtacgatc cgggcccttt gctcacggcg acggcatcga gctgcgcgaa agacaggccg
     3181 gcatctttca tcacttcgtc acagaaaacc gtcagcacct ctgcatgctt attgggttcg
     3241 cggatctcgc ggatggaaac gatctctccg cgggcagaca atgccacgga gcaggtagcg
     3301 gccgacgatt cgatcagcag gatcatggaa aatggcggaa cgcttttccg cggaataaaa
     3361 attagtcttc gatcgtggaa agcgattcct tcgagacctt ttccaccttc gcgccgttgt
     3421 aaagcgaagt gctgatggcg cggaaaggtg cggaaaccac ttcatctcct tccttgatgc
     3481 cggaaatgat ctggatgtac atgttgtctt ccacgcccgt aacgacggga atcagcttca
     3541 cttttccatc ggcgtaaacg aaaacacatt cgatcgattt cgtctcggcg gttttggtat
     3601 tgtccttcgt gtcatgcacg acgatgtcct ggttttcatc tccgcccgaa ggcttctcct
     3661 cttttttggc gaccgcgttc ggtgatgcgg tgtcggtacg tgtcgtgacg gaaataatgg
     3721 gaacggaaat cacatcccgc acggaattgg tcatgatctc gacggaagcc gacatcccgg
     3781 gaagaaatac gtaacggtcg ggatgctgcg ggtcgataag gtcggaatag gaatcgcgca
     3841 ggatgcggat cttcacctgg aagttcgtca cctggtccgt ggtgagtccc gtcgtgttcg
     3901 cggagttggc gacttccgtc accacgcctt tgaacttgcg gttgttgtgc gcatccactt
     3961 cgatcatcgc ggtgtcgcct tctttcacac gcaggatgtc attttcattc acgtccacct
     4021 gtacttccat ttcatcgaga ttggccagcc gcatgatctc ggttccttcc atctgtgcag
     4081 tcccgacgac gcgttcgcct tttttcttgt tcaggacaga aacggttccc gtcacgggcg
     4141 catagatatt cgttttggcg agattgtcct gcgcctgctt gagcgacgct tccgcattgc
     4201 tcacattcga ttcggcgctg tgcacgcttt gggttgcggc gtccacgtcg gcgagcgcgc
     4261 ttttgtacgt ggcgttcgcc tggtcgaatt ccgcatcgga aatggcgccc tggtcgtgca
     4321 gctttttgtt gcgcgtgaaa gaagcttccg cgttgttgag gttggctttc gactggtcga
     4381 gccgggcgcg gctgttcgca agattggcat gtgcgccgtc gacggaagcc tgcatctcgt
     4441 tcacggtcga ctcgtaaatg acgggattga tgcgaagcag gaggtcgcct tttttcacgg
     4501 tatcgccttc tttcacgtcg agctcgacga tctcgccgga tacgtcggaa ctgatcttca
     4561 cctcgacctc aggctgcact tttccgctgg cggaaacaac ttccgtgata tttctcttct
     4621 gtgctttttc cgttgccacg cgctcggttc ctttcccgga acctttcgtg agcatgacga
     4681 cggtgatcag cactgcaaca atgattcccc cgatgatcag tatgcgtttc atagtgatgt
     4741 tatttcagaa ggtgagtggt ttgccctggt aatagtcaag cactttcatc cggaaaatgt
     4801 aatcgtactt ggcctggatg aggtccgact gcgctttcgt gagcttgttc ttggcgttgt
     4861 tgtaatcgat cgagttgacc gccccgacgt cgaatttctg ctctgtgtaa ttgaacgatt
     4921 cctgcgccgc atcgacggct ttctgcgaag cctggtattt ctgcagcgcg gcctgcgcgt
     4981 cggcgtacgc ctgctgtatg ttcttcatca gctgctgctg cgaaagatcg aggctgagct
     5041 gcgcgttctc gtaattgatc ttcgcccggt cgacgttgtc cattacctgg aaacggttga
     5101 agatcgggac cgtcacctgc acgccgaagc ttttgttcac gttgttgttg aactggtccg
     5161 agaacggcgt cgtgatgtac gtggtcgtgt aattcgggat cagcacgtaa ccgccattgg
     5221 tggtgacgcc cgccgtgtcg taccccgaat agctcgtgcc ggtgaccgtt ttcgcagcgc
     5281 cggaataacc tgttccgatg gaaccctgga tggtcacggt cggacttaac ccgccgtagg
     5341 ccacgtccac gcctttgtcc gcgctcttca catccatctg cgattttttg ataccggcct
     5401 ggttgttcat cgccgcctgg aaaataagat caggtgttgt gttcagcagg ctttcattcg
     5461 gaacatcgat gatgggttta acgatggtga agccggacgt cgaatcgagg ttcatcatct
     5521 gcgtcagcgt gaggtaagcg atggtgagat tgttctgcgc cgttacaagt gaaacctgtt
     5581 ccgtggccag ctgcgactgg agatcgagca gcgagccttt agctgcagcg cccgcatcca
     5641 cgagcttctg cgtcctgtcc acctgcgcct gggtgaggtc gacctgctgc tgcgacacgc
     5701 cgagcgcttc ctgcgcgtaa agcacgttga ggtaagcgct cgccacattc atgccgatgt
     5761 cgtcctgcgt ctgctgtacc tggtagcggc ttccctgcaa cgcatactgg tcctgcttca
     5821 tcgtgttgaa attctgcatg ccgttgaaga ccgtccagtt gctgctgagg taaaagtttt
     5881 cggaaagcac catgctgttg gcgaacgtat tcgtgaaacg gtcgacggtc cttccgtact
     5941 ggtaggtgtg cgaagcaaaa ccgttcacgt tgggaagcat gttgccctgg ctctgccgga
     6001 ggttcacctg cgcgagctgc acgttgagca gttgctgctg cacggtgatg ttgtgcacct
     6061 gtgcgtacgc aatgcattgc gtaagattcc agggttgcga attatcggcc gggaacaggg
     6121 cggagtcgct gtttgcagcc acgacgcgga gcagcgaagt gtcggcagcg ggatccattt
     6181 tcacgaaggt ctgcccggaa gcagtaagcc ccgtcagaac ggcgaaaagg aggaggaccg
     6241 gtgtgagaag aattcgtttc atgttcagat tggactgcaa agttatccaa aagacgttca
     6301 cgggaatccc tttcgtacct ttaatgaaat gaactttctc gcccactttt acctttccgg
     6361 caccaatgaa aacctgctgt cgggaaattt tatcgcggac gcggtaaaag gaagcgatct
     6421 ttccgcgttc ccggaaggaa tacgcgaggg tatcatcatg caccgccaga tcgatcattt
     6481 cacggatcgt cacccggtgg tgatgaagag cataaaaagg ctgcgcccgg actttcatca
     6541 ttattcgccc gtcatcaccg acattttttt cgatcacttt ctcgcgacga actggaatga
     6601 atactcggag gaacctcttg ccggctacgc cgcgcgcacc tacgccatcc tggaaaagta
     6661 cgtggatgtt ttcccggaaa aatcgaagcg cattctgccg ttcatgaagc agcacgactg
     6721 gctgacgatc tacagccggg tggaaggggt gcacaacgtg ctcacgggaa tgtcgaggag
     6781 aacgacgttc gattcgggaa tggagcgcgc cgcagaagcc ctggtcaggg attacgatgc
     6841 attcggggac gacttccgca aatttttccc cgacatcatc gcacacacac agaagttccg
     6901 ttcataagcc tgtggataac ggcaggagaa ttcttgctgg gttcattttc ggttaacaaa
     6961 cagggcggat ttttaccggc aaatggaaca gctccggtta tccctcatca tccttttcgc
     7021 ggtcgcagga atttttacgc tgatcggttt tctccccgtg gaatttcagc cggctgattc
     7081 tttcgacgac gaggaagtcc aggaagaagc cagccagaac gaaagtcccg aaaaaagcgg
     7141 ggccgctgca acgtccgcca cgtaatgcgc gcgctgcacg accagcagga ttcccaccgc
     7201 cagcgcggaa agcgcgaacg tgtatttcag ccatgatctc cccaatacgg gaatgaacat
     7261 gacgatcgta gctacgtggc cggaaaagaa aagatctttc aggttcgcac gcccgttgta
     7321 gaatgtcgat tgcaggaaag gatcgtgcag cgggatgatt ccgccgggcg gatcgagcgg
     7381 aaaaaccagc agtgaaattc cgcgcagcag ctggagaaag gcatacgccc gtatcatcag
     7441 cagcagtttt tcgggatttt gtgctgcaat gatcacaccg gcgatgatcg tggaataggt
     7501 aagcgggaaa ataattgctg aaaaatcatg tgttgacgcg atcccgtcca gccagccttt
     7561 ttcaatgatg agaccgtcgc ggttttccac gaacgccagc acgaaaaaaa atcccgcaag
     7621 gacaacggca aggaacagga atgaaatgag cagctgtttc cggaaggaag catcggcccg
     7681 gatcttttcc caggttgttt gcttcagacc gtgtgatgta attttcattc ggcaaactgc
     7741 aatggcaaac cgtcaaccgg gaaacttaaa ggctcatcat ttccttcagc ttcacggcct
     7801 gccttcttga aatttccacc tgctcgccgc cgcgcagctt caccagcagt ccgccgttga
     7861 accagctttc gatgttttcc acccagcgca ggttgatgat gtgtttccgg ctggcacgga
     7921 agaacgcttt attgttcagc cgttcgtcga ggtaattcag cgaacgcagg ataagcggtt
     7981 tgttcgttcc gaaatacagg cgcacgtaat ttccttccga ttcgaacagg cggacatcac
     8041 cgagcttcac aaaccagcag cgttccccat ctttcacaaa aacctggtcg tcctcgccga
     8101 gcgtgttgcc cgtgtctttc gcctcttttt tctcgttcag gtgcgaattg atcttgcgga
     8161 tcgcatccga aagacgcgcg ggctgcacgg gcttcagcag gtagtcgagc gcattcacct
     8221 cgaatgcacg tatggcgaat tcgtcgtgcg cggtgacgaa cacgacctcc ggcacgaatg
     8281 tgatctcttc gagcaggtcg aagccgttct ttcccggcat ttgtatgtcc aggaaaatga
     8341 catccggctt tacgttgttg atggtttccg ctgcggattt tacgtcggcg cattccccgg
     8401 cgatctccac accgggatac gtggacagca tggatttcag ctcctgccgc gcaagacgtt
     8461 cgtcgtcgat gataactgct ttcatgcgtt ataggatttg ggtatgaata attcagtgat
     8521 gacggttccg ttgttctcgt tcgcgatgga aaatttcgcg gtatcgccgt acagcagcgc
     8581 cagccgctcg agtgtgttct tcattccgaa gccggactct gatttttttt ccgcgtcgta
     8641 ggttccggaa ttgcgtatgg tgatcagggt gccgctgtcg ttgttctgcg atgctatttc
     8701 cagcttcccg cccccgggta aacgcgcgat gccgtgcttg atgccgttct ccaccagcgt
     8761 ctgcaccatc agcgggggaa cttcgcatg
//
LOCUS       DASTSF010000257         2946 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_455494,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000257 DASTSF010000000
VERSION     DASTSF010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2946)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2946
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_455494"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2855)
                     /locus_tag="VFU15_07475"
     CDS             complement(<1..2855)
                     /locus_tag="VFU15_07475"
                     /inference="COORDINATES: protein motif:HMM:NF017393.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M43 family zinc metalloprotease"
                     /protein_id="HEU4717656.1"
                     /translation="MKYFFAGVLMLALFHLPAQTGTANDFPSCFSDELRMHKLAQDPG
                     EPQRVDQMEQAILQWTLAANATRQMASVYVIPVVVHIIHNNGPENISNAQAQAGIQDM
                     NDAFANTGPYQDPNGVNTGIQFCLAQQDENGNFTTGIDRVVSPLTNVTVETDDAALKN
                     LVRWDPTRYLNIWVVNDITSISAGPGVAGYAYLPTSHGMPEDGIVVEAHYFGTNTDNS
                     KVAVHEAGHYLGLYHTFEGGCVNNNCLTDGDRVCDTPPDQSTAAVNCGTPVNTCVSDE
                     DDLSANNPFRPVSAGGLGDQPDQIANYMDYGFQSCQRLFTDGQKTRMITALTTTRASL
                     LSSIGCQPLCSSPISAGFNMSASTITAGGTVNFTNTTSGATSYTWYIGSQQFATTTNA
                     SYVFTTPGYYTVTLQASNNDMTCDQSVTQQVHVLCGAQSAFTVSPQGPYSPGATIIFS
                     DASAGATSYEWVLDGISQSNATTFSQTFNTPGNHYIYLVTGNGQCSDTSASYFFQVGN
                     CGEAGTITNWLLPRMSLHFSGTSLPLTGPSPIPQPPMYGTECTSSYSDANGNLLLFTD
                     GLTVWNRNQQPMPNGTGLMGAASSTQSCIIVPEPGSSTRFYIFTTDCFENSFANGLHY
                     TIVDMTLDGGLGDVVTASKNVPVMTGNSEHVSATYHSNRQDIWIATSSIDGSQVYAYL
                     LTSAGLSSTPVISATGSASGQGMGAIKFSHDGHRVAYCIDNVFTPPNRVRVCDFNNTT
                     GQCTNSFDITYSPTSDERPYGIEFSPDNSKLYVAILIYGKVYQYDLSAGGPSAIVASQ
                     MQIDPYTSVQTSSYAHITLGPDGRIWVDRAQLHIDYIPDPNLAGTACGYVSDGLIIPG
                     QYGSIGWCMPNFLQGIELWSTPRISGPREVCNNSQAQFKVAYTSSGDSAVWSHTGPGS
                     ISNITDSTLILSTSAIGEVDTLMMQYFGYCGAIFDTF"
BASE COUNT          649 a          748 c          862 g          687 t
ORIGIN      
        1 atgaacgtat cgaaaatggc gccgcagtag ccgaaatatt gcatcataag tgtatcgact
       61 tctccgatgg cgctcgtcga gaggatcagc gtgctgtccg taatattcga aatgctgccg
      121 ggacctgtgt gcgaccacac agcggaatcg cccgatgaag tatatgcgac tttgaactgg
      181 gcctggctgt tgttgcaaac ttcgcgcgga ccggaaatgc gcggtgtcga ccagagctcg
      241 atgccctgga ggaaattcgg catgcaccag ccgatcgaac cgtattgacc cgggatgatc
      301 agtccgtcgg aaacgtaacc gcaggctgtt cctgcaagat tgggatccgg tatgtaatcg
      361 atgtgcagtt gtgcgcggtc gacccagatg cggccgtccg gtcccagcgt aatatgtgcg
      421 tagctgctcg tctgcacgga agtatacgga tcaatctgca tttgtgatgc aacgattgca
      481 gaaggtccgc ctgccgacaa atcatactgg tatacttttc cataaatgag gatcgcgaca
      541 tacagtttcg aattatccgg cgagaattcg atcccgtaag gacgttcgtc ggaagtcggg
      601 ctgtacgtga tgtcgaaact gtttgtgcac tgccccgtgg tgttgttgaa atcgcacaca
      661 cgcacgcggt tcggtggtgt gaaaacgtta tcgatgcaat aagccacgcg gtgaccgtca
      721 tgtgaaaact tgatcgcgcc cattccctgg ccgctggcag aaccggtggc ggaaatgacc
      781 ggtgtggaag aaagtcccgc tgatgtgagc aggtaagcat acacctggct tccgtcgatg
      841 gaagacgtgg cgatccagat gtcctggcgg ttgctgtggt acgtcgccga cacatgctcg
      901 ctgtttcctg tcatcaccgg aacgttcttc gatgctgtaa cgacatcgcc aagtcctccg
      961 tcgagcgtca tgtcgacgat cgtgtaatgc aaaccattcg caaaggaatt ttcgaaacaa
     1021 tccgtcgtga aaatgtaaaa gcgtgtcgat gatccgggtt ccggaacaat gatgcagctc
     1081 tgcgtggagg atgcagctcc cataagtccc gttccgttgg gcatgggctg ctggttgcgg
     1141 ttccagacag taagaccgtc cgtaaacaga agcaggttgc cattggcgtc tgaataagac
     1201 gaggtgcatt ctgttccgta catgggaggc tggggaatcg ggctgggccc ggtgagcggg
     1261 agactggttc ccgaaaaatg gagtgacatc cgcggcagga gccagtttgt gatcgtgcct
     1321 gcttcgccgc agttgcccac ctggaaaaaa tacgaagcgg aagtatcgct gcactgcccg
     1381 ttgccggtta cgagatagat atagtgattg ccgggtgtgt tgaacgtctg gctgaatgtg
     1441 gttgcattcg actggctgat gccgtcaagc acccattcat agctggttgc accagcggaa
     1501 gcgtcggaaa aaatgatcgt cgcgcccggg ctgtagggtc cctgcggtga tacagtaaac
     1561 gcggactgtg cgccgcagag aacgtgaacc tgttgcgtga cggactgatc acaggtcatg
     1621 tcattgttgg aagcctggag cgtgaccgta taataaccgg gtgtggtgaa aacatagctc
     1681 gcattcgttg ttgtcgcgaa ctgctggctg ccgatgtacc acgtgtaact tgtcgcgccg
     1741 cttgtcgtgt tggtgaaatt taccgttccc ccagccgtga tcgtggatgc gctcatgttg
     1801 aacccggccg agatcgggct gctgcaaagc ggctggcacc cgatggaaga cagcaggctg
     1861 gcgcgcgtgg tggtgagcgc tgtgatcatg cgtgtttttt gcccgtccgt gaaaagacgc
     1921 tggcacgatt gaaaaccata atccatgtag ttcgcgatct ggtcgggctg atcgccgaga
     1981 ccgcctgcgc tcacgggacg gaacgggtta ttcgcggaaa gatcgtcttc atccgacacg
     2041 caggtgttca cgggtgttcc gcaattgaca gcagcggtcg actgatcagg cggcgtatcg
     2101 cacacgcggt cgccatccgt caggcagttg ttgtttacgc agccgccttc gaacgtgtga
     2161 tacaggccga gataatgtcc tgcttcgtga accgcgactt tcgaattgtc ggtgttggtg
     2221 ccgaaataat gcgcttccac cacaattcca tcctcgggca ttccatgcga tgtgggtaga
     2281 taggcgtagc ccgccacccc gggtccggcc gagattgaag taatatcatt caccacccag
     2341 atgttgaggt agcgcgtcgg atcccatcga acaaggttct tcagcgcggc atcgtccgtt
     2401 tcaacggtaa cattggtaag cggtgaaacc acgcgatcga tgccggtggt gaaatttcca
     2461 ttctcatcct gttgcgccag gcagaattgt attcctgtgt tcacgccgtt cggatcctgg
     2521 taaggtcccg tgttggcgaa cgcatcattc atgtcctgta tgccggcctg tgcctgcgca
     2581 tttgaaatat tctccgggcc gttattatga atgatgtgca ccacgaccgg gatcacgtaa
     2641 acggaagcca tctggcgcgt tgcgtttgcc gccagtgtcc attggagaat ggcctgctcc
     2701 atctggtcca cacgttgcgg ttcaccggga tcctgcgcca gcttatgcat acgaagttca
     2761 tcggaaaaac aggatggaaa gtcatttgcc gtaccggtct gcgcgggcag gtgaaaaagt
     2821 gcgagcatga gcacgcctgc aaaaaaatat ttcatgatct ggatgtgttt gctgaagaac
     2881 aggtgccatc ccgtcttcat tcaaacttat cacgctaaat ttatcccggc aaataaaaac
     2941 aaccgt
//
LOCUS       DASTSF010000258         2481 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_945298,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000258 DASTSF010000000
VERSION     DASTSF010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2481
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_945298"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            368..2116
                     /gene="aspS"
                     /locus_tag="VFU15_07480"
     CDS             368..2116
                     /gene="aspS"
                     /locus_tag="VFU15_07480"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010600546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEU4717657.1"
                     /translation="MLRTHTCGELRLADAGKKVTLCGWIQVSRDFGGLTFVDLRDRYG
                     ITQLSFNMAVNADLCEQARKLGREFVVQVSGEVRERENKNPKIPTGEIEIVVSGLNIL
                     NGAKTPPFTIEDETDGGDDLRMKFRYLDIRRNPVRESLLLRHRLAQETRKYLSDQEFI
                     EVETPVLIKSTPEGARDFVVPSRMNPGQFYALPQSPQTFKQLLMVAGMDRYFQLVKCF
                     RDEDLRADRQPEFTQIDCEMSFVERADILGMFEGLVRHLFKTVKGIDIGTLPHMTYAD
                     AAKYYGSDKPDTRFEMKFVELNDVVKGKGFKVFDDAELVAGICAKGAAGYTRKQIDEL
                     TEFVKRPQVGAGGLVYARWNDDGMKSSVDKFFSAEELQKWMNAFGAEKGDLVLILAGA
                     KEKTRKALCELRLEMGTRLGLRDKNTFSCLWVIDFPLLEYNEEEKRWFAMHHPFTSPL
                     PQDIAKLDDVSQYGDIRANAYDMVINGVEVGGGSIRIFDRGLQSKLFEVLGFTKEEAQ
                     AQFGFLMNAFEYGAPPHGGIAFGFDRLCSLFGGADSIRDFIAFPKNNSGRDVMIDSPG
                     ALDEKQLKELNIRTTV"
     gene            complement(2226..>2481)
                     /locus_tag="VFU15_07485"
     CDS             complement(2226..>2481)
                     /locus_tag="VFU15_07485"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEU4717658.1"
                     /translation="HENTLPGNLQVSPNPASDVLHVTWDAGYSVVQIDIYDVSGKRVM
                     SSAVNGQNADLNISELPDGVYNMKITGGNGQVTDYRFMKN"
BASE COUNT          589 a          631 c          703 g          558 t
ORIGIN      
        1 atgacgatga gtgggaggac ggagaaattt atgatcccaa ttccggcgac acgtatgatt
       61 cggtggtgtg gatggaagac ggcgtgctga aagtgaaagg ctacgttctc ttcgaatggc
      121 tgggccggac gatggaattc aaacgtgttt gaaaaatgca ggagcgggac cgtttctttg
      181 ggaatggtta ttctttagtg atttaaagat tacagcatcg gaatagtttc cccaaaaccc
      241 caaagtttcg ttctggaatt ggaattcctt aaaagggata agaacgtacc ggcacggaac
      301 attcctcttt gcccaagact ccgaacctcc atctatttcg taatttcacg acccaattct
      361 catttctatg ctacgtactc acacctgcgg cgaactgcgt ctcgccgatg ccggaaaaaa
      421 agtaacactc tgcggatgga tacaggtttc gcgcgacttc ggcggactca ccttcgtcga
      481 tcttcgcgac cgctacggca tcacgcagct ttcgttcaac atggcggtga atgcggatct
      541 ctgcgaacag gcgcgtaagc tcggaagaga atttgtcgtg caggtgagcg gtgaggtacg
      601 cgagcgcgag aacaagaatc cgaaaattcc caccggggag atcgagatcg tcgtctccgg
      661 gctgaacata ctgaatggcg cgaaaacgcc cccgtttacc atcgaagatg aaacggacgg
      721 aggcgatgat cttcgcatga agttccgtta cctcgacatt cgccgcaatc ctgtgcgtga
      781 aagcctgctg ctgcgtcacc gcctggcgca ggaaacgcgc aaatacctca gcgaccagga
      841 attcatcgag gtggaaacgc ccgtgctcat caagtcgaca ccggagggtg cgcgcgactt
      901 cgtcgtgccg tcgcgcatga accccgggca gttttacgcg ctgccgcaat cgccgcagac
      961 gttcaagcag ctgctcatgg tggcgggcat ggaccgttat ttccagctcg tgaaatgttt
     1021 ccgcgatgaa gatcttcgcg ccgaccgcca gcccgagttc acgcagatcg actgcgagat
     1081 gagcttcgtg gaacgtgcgg atattctcgg catgttcgaa ggtcttgtcc gccatctttt
     1141 caaaacggtg aaaggcattg acatcggcac attgccgcac atgacttacg ccgatgcggc
     1201 aaaatattac ggcagcgata aacccgatac gcgtttcgaa atgaaattcg tggagctgaa
     1261 cgacgtggtg aagggaaaag gattcaaagt gttcgacgat gcggagctgg ttgccggcat
     1321 ctgcgcgaaa ggtgcggccg gttatacgcg caagcagatc gatgagctga cggaattcgt
     1381 gaaacgtccg caggtggggg caggcggatt ggtgtacgca agatggaacg acgacggaat
     1441 gaaatcgtcc gtggacaaat tcttcagcgc ggaagaattg cagaaatgga tgaacgcgtt
     1501 cggcgcggaa aaaggtgatt tggttttgat cctcgccggt gcgaaagaaa aaacgcgcaa
     1561 agcgctgtgc gagctgcgtc tcgaaatggg tacgcgtctc ggcctgcgcg acaagaatac
     1621 gttttcctgc ctgtgggtga tcgattttcc gctgctcgaa tacaacgaag aagagaaacg
     1681 ctggttcgcg atgcatcacc cgttcacctc gccgctgccg caggatattg cgaagctgga
     1741 cgacgtgtcg caatatggcg atatccgtgc caacgcttac gatatggtga tcaacggcgt
     1801 ggaagtcggt ggcggatcga tccggatctt cgaccgcggt ctgcaatcga aactgttcga
     1861 agtgctcggc ttcacgaaag aggaagcaca ggcgcagttc ggatttttga tgaacgcctt
     1921 cgaatacggc gcacctccgc acggtggcat cgcgttcggt ttcgaccgtc tctgctcgct
     1981 tttcggcggc gcagattcca tccgcgattt catcgccttc ccgaaaaaca actccggccg
     2041 cgacgtgatg atcgattccc ccggcgcgct cgatgaaaaa cagctgaagg agctgaacat
     2101 caggacgacg gtctgatgaa aaaatgaagc gtccccggac taacgtctgc cggttaattt
     2161 ccgtaagata agttcacaaa aaagccccgc attgctgcga ggctttttct gttttgttga
     2221 accgcttagt tcttcatgaa gcggtaatcc gtcacttgtc cgtttccgcc ggtgatcttc
     2281 atgttgtaga caccatcagg aagctcggaa atgttcaggt ctgcattttg tccgttaact
     2341 gccgagctca tcacgcgttt tccgctgacg tcatagatat cgatctgcac tacggaatat
     2401 cctgcatccc aggttacgtg cagcacgtcg gaagccgggt tcggggaaac ctgcaggttt
     2461 ccgggaagtg tattctcgtg a
//
LOCUS       DASTSF010000259         4587 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_448646,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000259 DASTSF010000000
VERSION     DASTSF010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4587
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_448646"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..118
                     /locus_tag="VFU15_07490"
     CDS             <1..118
                     /locus_tag="VFU15_07490"
                     /inference="COORDINATES: protein motif:HMM:NF024759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HEU4717659.1"
                     /translation="NHYPQVINYLAVSKCPIGLLVNFGENSLRYKRIALTQK"
     gene            149..1642
                     /locus_tag="VFU15_07495"
     CDS             149..1642
                     /locus_tag="VFU15_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014177229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEU4717660.1"
                     /translation="MTTKTKSNSRQEQPALDFTSGWNYAPSPESTDHVRIDKKYDHFI
                     NGEFVKPHSGKYFDTISPSTEKKLSEIASGDEHDVDKAVKAARNAYSNVWKKMKPAER
                     GKYLYRIARLIQERSRELAVIESMDGGKPIRESRDVDVPLAAAHFFYYAGWADKLEYA
                     FPNRKPEPLGVAGQIIPWNFPLLMAAWKIAPALACGNTVVLKPAETTPLTALKLAEII
                     RDAGLPDGVVNIINGAGKTGAALVHHKGVDKIAFTGSTDVGKIIQKAIAGTGKKATLE
                     LGGKAANIVFEDAAIDQAVEGIINGIFFNQGHVCCAGSRLFVQESVADEVVRRLKDRM
                     ETLIVGDPLDKNTDIGAINSREQLEKIKFYLDIGVSEGAELWESSCALPPKGFFCRPT
                     VFLNASQSHRIVQEEIFGPVLAVQTFRTIEEVVEKANNTPFGLSAGVWTDKGSKIFNL
                     TSKMRAGVVWANTYNKFDPTSPFGGYKESGFGREGGLHGLMPYVNLK"
     gene            1680..2564
                     /locus_tag="VFU15_07500"
     CDS             1680..2564
                     /locus_tag="VFU15_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEU4717661.1"
                     /translation="MSRLNVLKTYKLYIGGQFPRTESGRYYPLHDKKKNVIADICLAS
                     RKDFRNAVVAARGAVAGWSGKSAFNRSQVLYRIAEMLEGRKAQFVEELMLQGATKANA
                     EKEVTLSIDRLVYYAGWCDKYTQVFSTVNPVQSSHFNFSLPEPTGVVAIIAPQDSSLL
                     GLVSVVAPVIAGGNVCIALASEKLPLCSITFAEVLATSDLPGGVVNILTGKISELFSH
                     MSSHMDVNALVYAAEDKLHIRGIQENAALNVKRVFVYSETDWTKEEAQNPYMILDTQE
                     IKTTWHPIENVGASGGKY"
     gene            2564..3367
                     /gene="cysQ"
                     /locus_tag="VFU15_07505"
     CDS             2564..3367
                     /gene="cysQ"
                     /locus_tag="VFU15_07505"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008760691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HEU4717662.1"
                     /translation="MSIGKAQREEWYALAVTAAFRAGEEIMKVYGSDDFGVEKKEDES
                     PLTLADREADEVIGRCLGVTGIPILSEEGAQIPYSERKAWNRCWIVDPLDGTKEFIKR
                     NGEFTVNIALVENGEPVFGVVYAPALDVVYVGEKGAGAWKGTSGDFRKTAQRALPLNP
                     VPQTFTMVGSRSHSSPETEAYVKEMEAKHGKVDFVASGSSLKFCLVAEGKAQAYPRFA
                     PTMEWDTAAGQAVLEAAGGKVFMWPSGQPLRYNREELRNGWFLATAAGK"
     gene            3459..4547
                     /gene="gmd"
                     /locus_tag="VFU15_07510"
     CDS             3459..4547
                     /gene="gmd"
                     /locus_tag="VFU15_07510"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006890984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEU4717663.1"
                     /translation="MAKTALITGVTGQDGAYLSEFLLKKGYTVHGIKRRSSLFNTHRI
                     DHLYHDPHEKGVSFHLHYGDLTDSTNLIRIVQDVQPDEIYNLAAQSHVKVSFETPEYT
                     ANADAIGTLRLLEAIRILRLESKTRFYQASTSEMYGLVQEIPQTEKTPFYPRSPYGVA
                     KLYGYWITKNYREAYGLYACNGILFNHESPVRGETFVTRKITRGVARIRLGLQDKLYL
                     GNLDAERDWGHARDYVEGMWLMLQQPAADDYVLATGRKISVRTFVEWAFNVAGIAIEW
                     KGKGVDEKGYDKATGKAIVEIDTKYFRPAEVDLLVGDASKAKKELGWEPKFSLEELVK
                     EMVMEDLKLFSRDKYLLEGGHSINQFHE"
BASE COUNT         1175 a         1201 c         1287 g          924 t
ORIGIN      
        1 caatcactac ccacaggtta taaattacct tgctgtttca aaatgcccga taggactcct
       61 cgtcaatttc ggtgaaaatt cattgcgtta caaacgaata gctttgacac aaaagtaaat
      121 ttgtgtccat cagcgtaatc agcgacccat gacgacaaaa accaaatcca attcccggca
      181 ggaacaacct gcgctcgatt tcacttcggg ctggaactat gcgccgtcgc cggaaagcac
      241 ggatcacgtc cgtatcgata aaaaatacga tcactttatc aatggagaat tcgtgaagcc
      301 gcattcggga aaatatttcg atacaataag cccgtcgacg gaaaaaaaac tttccgagat
      361 cgcctccggc gatgagcacg atgtggacaa ggcggtgaaa gccgcgcgca acgcgtactc
      421 caacgtgtgg aagaaaatga agccggcgga gcgcggaaaa tatttgtacc gcattgcccg
      481 cctcatccag gaacgttcgc gggagctcgc cgtcatcgaa tcgatggacg gagggaagcc
      541 gatacgcgaa tcgcgtgacg tggatgtccc gctcgccgca gcgcattttt tctattatgc
      601 aggatgggcc gacaagctgg aatacgcttt tccgaaccgg aaaccggaac cgctgggcgt
      661 ggccggacag atcattccct ggaatttccc gctgctcatg gcggcatgga aaatcgctcc
      721 ggcgctggcc tgcggaaata cggtggtgct gaagcccgcc gaaaccacgc cgctcactgc
      781 gctgaagctc gctgaaatta tccgcgatgc gggtctgccc gatggcgtgg tgaacatcat
      841 caacggcgcc ggaaaaacag gagctgctct cgtgcatcac aaaggcgtgg acaagatcgc
      901 gttcaccggt tccaccgacg tcggaaaaat tatacagaaa gcgatcgccg gaacgggaaa
      961 aaaagccacg ctcgagctcg gggggaaagc tgcgaacatc gttttcgagg acgctgcgat
     1021 cgaccaggcc gtggaaggca tcatcaacgg aattttcttc aaccaggggc atgtctgctg
     1081 cgcaggttcg cgccttttcg tgcaggaaag cgtcgcggat gaagtggtcc gccgcctgaa
     1141 agacaggatg gaaacgctga tcgtcggcga tccgctcgac aagaacacgg acatcggcgc
     1201 gatcaattcg cgcgagcagc tcgagaaaat aaaattctac ctcgatatcg gtgtgagcga
     1261 aggcgccgaa ttgtgggaat cttcctgcgc gcttcccccg aaaggatttt tctgccgccc
     1321 aaccgttttc ctcaacgcgt cgcaatcgca ccgcatcgtg caggaagaaa ttttcggacc
     1381 cgtgctcgcc gtgcagacgt tccgcacgat cgaggaagtg gtggaaaaag ccaacaacac
     1441 gccgttcgga ctttccgccg gtgtgtggac cgacaagggt tccaaaattt tcaacctcac
     1501 ttcgaaaatg cgtgccggtg tcgtctgggc gaacacatac aacaagttcg atcccacttc
     1561 tccattcggc ggatacaagg aaagcggctt cggaagagaa ggcggactgc acggattgat
     1621 gccgtacgtg aacctgaaat aatattaacg attgatcatt tattattcaa ccttacagca
     1681 tgtcacgact caacgttctc aaaacctata agctttacat cggcggacaa tttccccgta
     1741 ccgaatccgg gcgttattac ccgctgcacg acaaaaagaa aaacgtgatc gcggatatct
     1801 gcctcgcctc gcggaaagat ttccgcaacg ccgttgtcgc tgcgcgtggc gctgttgcag
     1861 gatggagcgg gaagagcgct ttcaaccgtt cgcaggtgct ttaccgcatc gcggaaatgc
     1921 tcgaaggaag aaaagcgcag ttcgtggaag aactgatgct gcagggcgca acgaaagcaa
     1981 atgcagaaaa agaggtcacg ctttccatcg accgtctcgt gtattacgcc gggtggtgcg
     2041 acaaatacac acaggtgttc agcacggtga atcctgttca gtcgtcgcat ttcaatttct
     2101 ccctgcccga accgaccggc gtcgtggcga tcatcgctcc gcaggattcg tcactgctcg
     2161 gacttgtttc cgtggtggcc cccgttattg ccggtggaaa tgtatgcatt gcgcttgcgt
     2221 ccgaaaagct gccgctgtgc agcattacgt tcgcggaagt gctcgctacg tccgatcttc
     2281 ccggcggtgt cgtgaatatt ctcacgggaa aaatttccga gctgttttcg cacatgtcct
     2341 cgcacatgga cgtgaacgcg ctggtgtatg cagcggagga caagcttcac attcggggaa
     2401 tacaggagaa tgcggcgctg aatgtcaagc gcgtttttgt ttacagcgaa acagactgga
     2461 cgaaagaaga agcgcagaat ccatacatga tcctcgacac acaggagatc aaaacaacgt
     2521 ggcacccgat cgagaatgtc ggcgcttccg gggggaaata ttgatgagca tcggtaaagc
     2581 acaacgggaa gaatggtacg cgctggcggt taccgcagcg ttccgcgccg gcgaggagat
     2641 catgaaagtg tacgggagcg atgatttcgg cgtcgaaaaa aaagaggatg aatccccgct
     2701 gacgctcgcc gatcgcgaag cggacgaagt tatcggcagg tgccttggcg ttacgggaat
     2761 tcccattctc agcgaagagg gcgcgcagat cccgtacagt gaacggaaag cctggaaccg
     2821 ctgctggatc gtcgatccgc tggacggaac gaaagaattc atcaagcgta acggggagtt
     2881 caccgtaaac attgcgctcg tggagaacgg cgaacctgtt ttcggtgtcg tgtatgcgcc
     2941 cgcgctcgat gtggtgtatg tcggtgaaaa aggagccggc gcctggaaag gaacatccgg
     3001 cgattttcgc aaaacagcgc agcgcgcatt gccgctgaat cccgttcctc aaacgttcac
     3061 gatggtgggc agccgctcgc attcctcacc ggaaaccgaa gcgtacgtga aagagatgga
     3121 agcgaagcat ggaaaagttg atttcgtcgc ttccggcagc tcgctcaaat tctgcctggt
     3181 cgccgaaggg aaagcacagg cctatccgcg cttcgccccg acgatggaat gggatacggc
     3241 agccgggcag gccgttctcg aagcagccgg aggaaaagtg ttcatgtggc cttccggaca
     3301 gccgttgcgc tacaaccgtg aagaattgcg caacgggtgg tttctcgcca ctgcagcagg
     3361 aaaataagct cccggtcctc gtgtgattcg atgcgttcac ctacgttgcc ccgccattta
     3421 cggaggaaaa cgtagttttg tgattcaacg caatcatgat ggcaaaaact gcactgatca
     3481 ccggcgtaac gggacaggac ggagcgtatc tcagtgaatt ccttctgaaa aaaggatata
     3541 cggttcacgg gatcaaacgg agaagctccc tttttaatac gcaccgaatc gatcaccttt
     3601 atcatgatcc gcatgaaaaa ggggtgtcgt tccacctgca ttacggtgat ctcaccgact
     3661 cgacaaatct catacgcatt gtccaggacg tgcagccgga tgaaatttac aacctcgctg
     3721 cgcagtcgca tgtgaaagtt tcattcgaaa cgccggaata cacggccaac gccgatgcga
     3781 tcgggacgct tcgcctgctc gaggccatcc gcatcctgcg gctcgaaagc aaaacccgtt
     3841 tttaccaggc atccacatcg gaaatgtatg gcctcgtgca ggaaattccg cagacggaaa
     3901 agacaccgtt ctacccgaga agtccgtacg gtgtggcgaa gctgtacggc tactggatca
     3961 cgaaaaatta ccgcgaagct tacggactct atgcgtgcaa cggcatcctg ttcaaccacg
     4021 aaagccctgt gcgcggagaa acgttcgtca cacgcaagat cacccgcggc gtggcgcgca
     4081 tcaggctcgg gctgcaggac aagctgtatc tcggaaatct ggatgccgaa cgcgactggg
     4141 ggcatgcgcg tgattacgtg gaaggcatgt ggctcatgct ccagcaaccg gctgcggacg
     4201 attatgttct tgccacggga agaaaaattt ccgtgcgcac attcgtggaa tgggcattca
     4261 atgtcgccgg tatcgccatc gaatggaaag gaaaaggggt ggatgaaaaa ggctacgata
     4321 aagcaacggg aaaagcgatc gtcgagatcg acacgaaata tttccggccg gcggaggtgg
     4381 acctgctggt aggcgatgca tccaaggcca aaaaagagct gggatgggaa ccgaagtttt
     4441 ccctggaaga gctcgtgaag gaaatggtga tggaagatct caagcttttc tcgcgcgaca
     4501 agtacctgct cgaaggaggc cacagcataa accagtttca cgaataggat attttcccgg
     4561 catgaacaag gagtcgaaga tttacgt
//
LOCUS       DASTSF010000260         4471 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1792941,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000260 DASTSF010000000
VERSION     DASTSF010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4471
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1792941"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="VFU15_07515"
     CDS             complement(<1..266)
                     /locus_tag="VFU15_07515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717664.1"
                     /translation="MQPEKKKIRHLRSHEIDRQRWDECIRRSFNGIIYAYSWYLDVVS
                     PGWEALADEKYETVMPLTWAKKMSVRYLRTPFFVQQLGVFSVNK"
     gene            complement(256..1563)
                     /locus_tag="VFU15_07520"
     CDS             complement(256..1563)
                     /locus_tag="VFU15_07520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEU4717665.1"
                     /translation="MSTITIYTHRITGRNRYIFRLYFRDLLGMEVRITDNAEEFLAAE
                     GVKLSYTTQPLKDELFFLSRNLLFETGISEQNITVFDWEGNKAFFSSGKTSALPFDLF
                     AAGFYLVSRYEEYLPHIRDRLDRFDAHQSLAWLHGFLHVPVVNRWAILLKEKILERFP
                     GTVFREEGYRFIPSIDIDNAWAYLEKGVMRTAGGYARSVFRMDFEDFRRRTQVLLHTR
                     KDPYDTYEYQLSLMKKTGVRPMYFFLVGDYGVNDKNVSTQRRKFRELIRHLADYSEIG
                     VHPSFASNTLPGKLQVEIQRLKNIVHREITNSRQHFLMLRFPNTYRNLIERDITDDFS
                     MGFANEPGFRAGICTPFFFYDLDLESETVLRVHPFTVMDATLNLYLKLTPEEAIAKIK
                     LLNEEVRKVNGTFMSLWHNETLSDEKEWKGWRIVYEELLKIAT"
     gene            complement(1560..2708)
                     /gene="wecB"
                     /locus_tag="VFU15_07525"
     CDS             complement(1560..2708)
                     /gene="wecB"
                     /locus_tag="VFU15_07525"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003357865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HEU4717666.1"
                     /translation="MIRLFTVTGARPQIIKAAAISRAIRSRFSDVMTETIIHTGQHYD
                     EEMSGIFFSELGIPEPVHNLRISEENDPVRTMIRRLSELFRQEKPDVALVYGDTNSTL
                     AGAFAANNCGIPLVHVEAGLRSFNTDMPEEFNRIATDHSSSLLFAPTDTAVANLEKEG
                     IAGAREGERAGVNNPVVCRCGDIMYDNSLHFASLARPLSQLPGMSDLAGKPFFLATVH
                     RNFNADDETRLSSVFRAFARLGEEQHAPVVVPLHPRTRKNISAPLRDEIEQARRKDFV
                     RILPPVGFLDMITLEKNARLVLTDSGGVQKEAYFFRKPCVVLREETEWVEIVRQGCAL
                     LSGTGTENILRAARELLERDEFRFAGLFGDGRAAEFICEKMIFHFSPQ"
     gene            complement(2715..3434)
                     /gene="radC"
                     /locus_tag="VFU15_07530"
     CDS             complement(2715..3434)
                     /gene="radC"
                     /locus_tag="VFU15_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016197070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEU4717667.1"
                     /translation="MEEEKTGQGYATQKMPIKTWAEDDRPREKFLLKGRHSLSDAELM
                     ALIIGSGSRDESALDLCRRILASCGNNLDRLGKMGTGEISSFRGMGNAKTISLLAALE
                     LGRRRNVQSAGSAVKIASSGDAYALLRSCLSDLAHEEFWVVYLNRSNIVVNREQVSRG
                     GMNATVVDPKIVFRKALSAGAANIILAHNHPSGAVRPSEQDMRLTRRLKEGAALLEIG
                     LLDHIIVGDNLYFSFADEGLL"
     gene            complement(3500..3754)
                     /gene="rpsT"
                     /locus_tag="VFU15_07535"
     CDS             complement(3500..3754)
                     /gene="rpsT"
                     /locus_tag="VFU15_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004917384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEU4717668.1"
                     /translation="MANHNSAKKRIRSNGAKRLTNRYQAKTTRNVIRKIRAEKDAKVV
                     SEMLPKVVSMLDKLAKKNVIHKNKAANLKSKIMKAAKAGK"
     gene            3821..>4471
                     /gene="mnmH"
                     /locus_tag="VFU15_07540"
     CDS             3821..>4471
                     /gene="mnmH"
                     /locus_tag="VFU15_07540"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015330283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HEU4717669.1"
                     /translation="MPTRLSIENFLLESTGIPVADVRSPAEFAGGHIPGAFNIPLFTN
                     EERAAVGTIYKQQGNEAAVLKGLEFVGPKMTAFILKARENAPGKKIAVHCWRGGMRSA
                     SMAWLFETAGMEVLLLSGGYKAYRNFVLSNTGRKFDLRVVGGETGSGKTDILHELARK
                     GEQVLDLEGLARHKGSSYGAIGQDPQPTVEQFENDFVHALTKLDPSRPVWIEDESRS"
BASE COUNT          979 a         1263 c         1175 g         1054 t
ORIGIN      
        1 tgcttgttta ccgaaaaaac accgagctgc tgcacgaaga agggcgtacg gaggtaacgg
       61 acgctcattt tttttgccca cgtcagcggc atcaccgttt cgtacttttc atcggcgagc
      121 gcttcccatc ccggcgaaac aacgtcaagg taccacgagt aggcgtaaat gatcccgttg
      181 aacgatctgc ggatgcattc gtcccaacgc tgcctgtcga tctcgtgcga acgcaggtgc
      241 cggatctttt ttttctcagg ttgcaatctt cagcagttct tcgtaaacaa tccgccagcc
      301 tttccattct ttttcatcgc tgagcgtttc gttgtgccac agcgacatga acgttccgtt
      361 cactttccgc acctcttcgt tcagcagctt tatttttgcg atcgcctcct ccggcgtcag
      421 cttcaggtaa agattcagcg tggcgtccat cacggtgaac ggatgaacgc gcagcacggt
      481 ttccgattcg agatcgagat cgtaaaagaa aaaaggtgtg cagatcccgg cacggaaacc
      541 gggttcgttc gcaaatccca tggaaaaatc gtccgtaata tcgcgttcga tcaggttgcg
      601 gtacgtgttc gggaatctca gcatgaggaa atgctgcctg ctgttcgtga tctcgcggtg
      661 aacgatgttc ttcaggcgct gaatttccac ctgcagcttc ccgggcaacg tattcgatgc
      721 gaatgaagga tggacgccga tctcggaata atccgcgagg tggcggatca gctcacggaa
      781 ttttcttcgc tgcgtggaaa cgttcttgtc gttcacgccg taatcgccga caaggaaaaa
      841 atacatcggg cggacgccgg tctttttcat cagcgaaagc tggtactcgt acgtatcgta
      901 cggatccttc cgcgtgtgaa gcagcacctg cgtgcgcctg cggaaatcct cgaagtccat
      961 gcgaaaaacc gaacgcgcat atccgcccgc cgtgcgcata acgccttttt caagataagc
     1021 ccacgcgtta tcgatatcga tcgacggaat aaaacggtag ccttcttccc ggaaaaccgt
     1081 gcccgggaat ctttccagta ttttttcctt cagcaggatt gcccagcggt tgacaaccgg
     1141 cacatgcagg aatccgtgca gccacgccag cgactggtgc gcatcgaaac ggtcgagacg
     1201 gtcgcggata tgcggcaggt attcttcgta acggctcacc aggtaaaagc ccgccgcaaa
     1261 aagatcgaac ggaagcgccg atgttttacc cgaagaaaag aaagccttat tcccttccca
     1321 gtcgaaaaca gtaatgttct gctcgctgat gcccgtttcg aaaagcaggt tgcgcgaaag
     1381 aaaaaacagc tcgtccttca gcggttgcgt ggtgtagctg agcttcactc cttccgctgc
     1441 aagaaattct tcggcattgt cggtgatgcg cacttccatc ccgagcaggt cgcggaaata
     1501 caggcggaaa atgtatctgt tccgtcctgt gatccggtgg gtgtatatcg tgatcgtgct
     1561 cactgcggac tgaaatggaa gatcattttc tcgcagatga attccgccgc gcgcccgtcg
     1621 ccgaaaagtc cggcaaaacg gaactcgtca cgctcaagca gctcacgcgc cgcccgcaga
     1681 atgttttctg ttcctgtacc tgacagcaaa gcgcatccct ggcggacgat ctccacccat
     1741 tccgtttctt cacgcaaaac cacgcagggc ttacggaaaa aatatgcttc cttctgcacg
     1801 ccgccggaat ccgtcagcac gagccgcgca tttttttcca gcgtgatcat atcgagaaaa
     1861 ccgacgggcg gaagaatgcg gacgaaatct ttcctgcgcg cctgttctat ttcatcccgg
     1921 agcggcgcag agatattttt ccgagtgcgc ggatgcagcg gcacgaccac cggtgcatgc
     1981 tgctcttcac cgagccgggc aaatgcgcgg aaaacggaag acaaacgggt ttcgtcgtcg
     2041 gcattgaaat tccggtggac ggtcgccagg aaaaacggtt ttcctgccag gtcgctcatg
     2101 cccggtagct gtgaaagcgg cctggcgagc gaagcaaaat gcaggctgtt gtcgtacatg
     2161 atgtcgccgc agcggcagac cacaggatta tttacgcctg ccctttctcc ttcccgcgcg
     2221 ccggcgatgc cttccttttc caggttggcg acagcggtgt ccgtcggtgc gaaaagcagg
     2281 gacgaggaat gatcggtggc gatccggttg aattcttccg gcatgtccgt attgaatgaa
     2341 cggagtccgg cttcaacatg caccagcggg atcccgcagt tgttggcggc aaatgccccg
     2401 gccagcgtgg aattcgtgtc gccgtacaca agtgcgacat cgggtttctc ctgccggaac
     2461 aattctgaga ggcgccggat cattgttctt acagggtcgt tctcttctga aatgcggaga
     2521 ttatgcaccg gctccgggat accgagctct gaaaaaaaaa tcccggacat ttcttcatcg
     2581 taatgctggc ctgtgtgaat gatcgtctcc gtcattacgt cgctgaaacg cgaccttatc
     2641 gccctgctga ttgcggcagc cttgatgatc tggggcctcg ctcccgtaac cgtgaaaaga
     2701 cgtatcattt tcttctataa caacccttcg tctgcaaagc taaaatataa attgtcgcct
     2761 acgatgatgt gatccagcag cccgatctcc agcaacgccg ctccttcctt cagtctccgc
     2821 gttaaacgca tgtcctgctc gctcggacgt acggcgccgg aaggatgatt atgcgcaaga
     2881 attatattcg ccgcccctgc cgaaagcgct ttgcggaaaa cgatcttcgg atcgaccacg
     2941 gtggcgttca tcccgccgcg gctcacctgc tcgcggttca ccacgatgtt cgaacggtta
     3001 agatacacga cccagaattc ctcgtgcgca agatcggaaa gacaggaacg aagcagcgcg
     3061 taggcatccc cgctcgaggc tattttgacg gcgcttccgg cgctctgcac atttctgcgg
     3121 cggcccagct ccagcgccgc aagcaggctg atcgttttgg catttcccat tcccctgaac
     3181 gagctaatct cgccggtgcc cattttcccg aggcgatcga ggttgttccc gcaggaagcc
     3241 agtatcctcc tgcaaagatc cagcgccgat tcatccctgc tcccggaacc gatgatcagc
     3301 gccatcagct cggcgtcgga aagcgaatga cggcccttga gcaggaactt ttcccgcggc
     3361 cggtcgtcct cggcccatgt tttgatcggc attttctgcg ttgcataccc ttgtcctgtc
     3421 ttttcttctt ccataaaaaa atgataaaaa aaatgcaccc tgttaagagt gcatttccca
     3481 gtaaaaaaat taagcgcgtt tatttgcctg cctttgcagc tttcatgatc ttgctcttca
     3541 ggttcgcagc tttattcttg tgaatgacgt tcttcttcgc cagtttgtcc agcatggaaa
     3601 cgaccttcgg cagcatttct gataccacct ttgcgtcttt ctcagcgcgg atcttgcgga
     3661 tcacgttacg cgtcgttttc gcctggtaac ggttggtcag tcttttcgct ccgtttgaac
     3721 ggatacgctt cttggctgaa ttgtgatttg ccatctcgct attgtttaaa tttgaggctg
     3781 cgaatatacg tctttttctc atatcccaaa cacctcctcc ttgcccaccc ggctttctat
     3841 cgaaaatttt ctcctggaat ccaccgggat accggttgca gacgtgcgct ccccggccga
     3901 attcgccggc ggccacatcc cgggcgcatt caacatcccg cttttcacca atgaagagcg
     3961 ggccgccgtt ggcacgatct acaaacagca gggaaatgaa gcggctgtcc tgaaaggcct
     4021 tgaattcgtg ggaccgaaaa tgacagcttt catactgaag gcccgggaga atgcgccggg
     4081 gaaaaagatc gccgttcatt gctggcgggg cggtatgcga agcgcttcca tggcctggct
     4141 tttcgaaacg gccgggatgg aagtcctgct gctctccggc ggatataaag cctaccggaa
     4201 tttcgtgctg tcgaataccg ggcggaaatt cgacctccgg gtagtcggtg gtgaaacggg
     4261 atcagggaaa acggatatcc tccatgaact ggcgcggaaa ggagagcagg tgctcgacct
     4321 cgaaggattg gcgcggcaca aaggctcttc gtatggcgcg atcggccagg acccgcagcc
     4381 gacagtcgaa cagttcgaaa acgatttcgt tcatgcgctg acgaagctcg atccgtcacg
     4441 gccggtatgg atcgaagacg aatcgcgcag c
//
LOCUS       DASTSF010000261         4757 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_743202,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000261 DASTSF010000000
VERSION     DASTSF010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4757
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_743202"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..668
                     /locus_tag="VFU15_07545"
     CDS             <1..668
                     /locus_tag="VFU15_07545"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEU4717670.1"
                     /translation="SWKEILPHVKRDTGYLRREHYEVLDLHNELVDPKTVKWSRYGKG
                     NLPFKFRQMTGDDNALGIMKFDFPNKYSVYMHDTNAKKYFRYETRAFSHGCMRLQDYM
                     DLAHFLIRDDSVKLPRDTFDFWTTTGDQRKINLKKPLPIHVRYFTCDVDTDGNVFLHS
                     DIYLRDRRMMRLLYADMKNDHRQEEKDSSPAGPADTKKKSDRKAVLNSPGDKSKSKKK
                     KKA"
     gene            complement(776..1963)
                     /gene="hflX"
                     /locus_tag="VFU15_07550"
     CDS             complement(776..1963)
                     /gene="hflX"
                     /locus_tag="VFU15_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019130651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEU4717671.1"
                     /translation="MPEISTQRAILIGVLDKRQDDRQLQEYMDELAFLATTAGIEPVK
                     RFTQKLDHPDPKFYIGSGKAKEIGEYINANAIDVVLFDDELSPSQQRNLEKEFQRKVI
                     DRNLLILDIFASRARTAHAKTQVELARNEYLLPRLTGMWTHLERQRGGTGTRGGAGEK
                     EIETDRRIVRDRIALLKEQLKEIDRQMTTQRKSRGQLVRVALVGYTNVGKSTIMNLVS
                     KSKVFAENKLFATLDTTVRKVVVGNLPFLLSDTVGFIRKLPHQLVESFKSTLDETREA
                     DILLHVVDISHPNFEDQFRVVKETLLEIGAGDKRTILVFNKIDAYRESPHETDPQDPA
                     ERRPLSLAELEKSWMSKMSDPCIFISATDKENIEQFRELLFDEVKAIHTKRYPYDKLE
                     FWK"
     gene            complement(2117..2464)
                     /locus_tag="VFU15_07555"
     CDS             complement(2117..2464)
                     /locus_tag="VFU15_07555"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEU4717672.1"
                     /translation="MKKIIFVFAGAVALFLFSCGNDKTQASGQDTSSGSATTVESADG
                     SSLYSSKCVSCHGDKGDKGFMNAADLSKSTLDHPGIVAVLKNGRNTMRSFSGELDDAQ
                     IEAVAKYVESLRH"
     gene            complement(2478..2918)
                     /locus_tag="VFU15_07560"
     CDS             complement(2478..2918)
                     /locus_tag="VFU15_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009578361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome B"
                     /protein_id="HEU4717673.1"
                     /translation="MEILAHIHSGLRWIVLILMLAAIFMAFSGMNGGKPFNRKPALFA
                     LISVHTQLLIGIILYILMMNSLNWDFSAAKNDPVLRFFTMEHITGMLLAIALITVGHS
                     RAKRGDTDKKKWKSIAIFYTIGLVLMLGAIPWPFMQKFHSLGWF"
     gene            3045..>4757
                     /locus_tag="VFU15_07565"
     CDS             3045..>4757
                     /locus_tag="VFU15_07565"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU4717674.1"
                     /translation="MKKLLCVISAFAVLHGAQAQLTTFQTNGAPDKRHTVFAFTNAHI
                     QADAETVIGNGTMLVQDGMITDIGTTVNIPKGAVVYDMQGKWIYPSFIDPYTTYGMPE
                     PKRSPWTPGPQYFSARDGAYGWNDALKPETDASAVFSSDKKAAEEMRGLGFGTVMVYS
                     NDGIARGTASCVTLNAEDGDNSVFVKQKVANCLSFDKGTSRQEYPSSLMGAIALLRQT
                     YYDADWYRTAKNRKEFNLSLEAWNNVQSLPQIFETTDKWNVLRADKVGDEFNIQYIFK
                     GSGNEYQRMNEMKNTNASFILPLNFPKAFDVSDPYDAEQIAYTDLKHWEMAPLNPGAF
                     EKYGINFALTTSDLEDKKDFWENLRKAIKFGLSEKAALKALTTVPAQLLGIADKTGAL
                     KKGLLANFIITNGNVFTDDAVIWENWVKGKQYILRDANTVDVRGTYSMTVGGNPMTLD
                     VEGDIFKPRGSFKKIDSIKGPVNVSCDGNRVDISWTYKKSGTTVLLTGLIDPKTKAMS
                     GSGQEPDGTWIKWSATQTAPFTPKPEPKDTTAPDIPSLDDVIYPFAPFGRTIKDSLEI
                     DRFQM"
BASE COUNT         1185 a         1306 c         1245 g         1021 t
ORIGIN      
        1 tttcctggaa agaaatactt ccgcacgtca aacgcgatac cggctatctc cgccgcgaac
       61 attacgaagt gctcgatctg cacaacgagc tcgtcgatcc gaaaacggtg aaatggagcc
      121 ggtacgggaa aggaaatctt ccgttcaagt tccgccagat gaccggcgac gacaatgcgc
      181 tcggcattat gaagttcgat ttcccgaaca agtacagcgt gtacatgcac gacacgaatg
      241 cgaaaaaata tttccgttac gaaacgcgtg ctttcagtca cggctgcatg cgcctgcagg
      301 attacatgga cctcgcgcat ttcctcatcc gcgacgacag cgtcaagctc ccgcgcgaca
      361 cgttcgattt ctggaccacc acgggcgacc agcgcaagat caacctgaag aaaccgctgc
      421 ccatccacgt gcgctatttc acctgcgatg tcgatacgga cggaaatgtt ttcctgcatt
      481 ccgacattta cctccgcgac cgccgcatga tgcgcctgct ttacgcggac atgaagaacg
      541 atcaccgcca ggaagaaaaa gactcatcgc ctgcaggtcc cgcagacacg aaaaagaaaa
      601 gcgacaggaa agcggtgctg aacagcccgg gtgacaaatc gaagagcaaa aagaagaaaa
      661 aggcgtaaac tattccggaa aattccgcgc accggtggat ctgccgcggg cagcactttg
      721 attccggccg ttatgactga actttacccg tgccggtgat cacatcatca ccgcatcact
      781 tccagaactc cagtttgtcg taaggatatc gtttcgtgtg aatggccttc acctcgtcga
      841 agagcaattc gcggaactgc tcgatgtttt ccttgtcagt ggcggaaata aaaatgcacg
      901 gatcgctcat tttgctcatc cagctttttt ccagctcggc gagtgaaagc ggccggcgct
      961 ctgccgggtc ctgcggatcc gtttcgtgcg gcgattcgcg gtaagcatcg atcttgttga
     1021 agacgagaat tgtccgtttg tcacccgcgc cgatctcgag cagcgtttct ttcaccacgc
     1081 ggaactggtc ctcgaaattc ggatgcgaga tgtcgaccac gtgcaggaga atatccgctt
     1141 cacgcgtttc atcgagcgtc gacttgaacg actccaccag ctggtgcggc agcttgcgga
     1201 tgaacccgac cgtatcggaa agcaggaaag gaagatttcc caccacgact ttccgcaccg
     1261 tcgtgtcgag cgtcgcgaac aatttatttt cggcgaatac tttcgatttc gaaacgaggt
     1321 tcatgatcgt ggattttccc acgttcgtat agccgacaag cgccacacgt acgagctgtc
     1381 cgcggctttt tcgctgcgtc gtcatctgcc ggtcgatctc tttcagctgc tctttcagca
     1441 gcgcgatgcg gtcgcgtact atacggcggt ccgtttcaat ttccttttcg cctgcgccgc
     1501 cgcgcgtgcc tgtaccgccg cgctggcgtt cgaggtgcgt ccacatacct gtcaggcgcg
     1561 ggagcaggta ttcgttgcgc gccagctcca cctgcgtttt tgcgtgggcc gtgcgtgcgc
     1621 gtgaagcgaa aatatcgagg atgagcaggt tgcggtcaat gaccttgcgc tggaattctt
     1681 tttcaaggtt gcgctgctgc gaaggagaaa gctcgtcatc gaacagcacg acgtcgatcg
     1741 cattcgcgtt gatgtattcg ccgatctctt tcgctttccc ggaaccgatg tagaatttcg
     1801 gatccggatg atcgagcttc tgtgtgaaac gtttcaccgg ttcgatcccg gccgtggtgg
     1861 ccaggaaagc cagctcatcc atgtattcct gcagctgccg gtcgtcctgc cgcttgtcga
     1921 gaacgccgat gagaatggct ctttgtgttg aaatttctgg cacgatggat caggcgttag
     1981 gcgcgatgcg ttgaaaagaa gcgctcatat cggaagcggg aaaagaataa cgacggtcat
     2041 cgggataaca tccggcaatt gcacaccctc tcttcatttg cagtccttcc gatcttctgt
     2101 cgccgaatgt ctcaagttaa tgcctcagcg actctacata tttcgccacc gcttcgatct
     2161 gcgcatcatc gagttccccg ctgaacgaac gcatggtgtt gcgcccgttc ttcagcacgg
     2221 ccacaattcc cgggtgatcg agcgtcgatt tcgaaaggtc ggccgcgttc atgaagcctt
     2281 tgtcgccctt atcgccgtga caggaaacac atttggaaga atacagcgaa gatccgtctg
     2341 cagactcaac cgttgtggcg gaacccgaag aagtgtcctg cccggaagct tgtgttttgt
     2401 cattgccgca ggaaaaaaga aaaagggcaa cggctcccgc aaaaacaaaa atgatttttt
     2461 tcatgaatgc tgctttttca gaaccagccg aggctgtgga atttctgcat gaaaggccag
     2521 ggaatcgctc ccagcataag gacaagcccg atcgtataaa agatcgcaat ggatttccat
     2581 ttctttttat ccgtatcacc gcgcttagcc cttgaatgac cgaccgtgat cagcgcgata
     2641 gcgaggagca tcccggtaat gtgctccatt gtgaagaagc gcagcacagg atcgtttttc
     2701 gctgcagaaa aatcccagtt cagggaattc atcatcagga tgtagagaat gatgccgata
     2761 agcagctgcg tatgcaccga gataagtgcg aacagcgccg gcttgcggtt gaacggtttg
     2821 ccgccgttca tgcccgagaa agccatgaag attgcggcga gcatgagaat gagaacgatc
     2881 cagcgaaggc cggagtgaat gtgcgcgagg atttccatag gattcagcgg ttttttatcc
     2941 tgtcaaaagt aaggaaaaat gcgccctcat taccgctcat ccccgcttcc cgccacttgg
     3001 tgaaaaaaag gatatttttg cttcttccag aaataaaatc cagaatgaaa aaactactct
     3061 gcgtgatctc cgcctttgcc gttctccacg gtgcgcaggc gcagctcacc actttccaga
     3121 ccaacggcgc cccggataaa cgtcacacgg tcttcgcctt caccaatgcg cacatccagg
     3181 ccgacgcgga aaccgtgatc ggcaacggaa cgatgctcgt gcaggacggc atgatcacgg
     3241 acatcggcac gacggtgaac ataccgaagg gcgctgtcgt ttacgacatg cagggaaaat
     3301 ggatctatcc ttccttcatc gatccttaca cgacatacgg aatgccggaa ccgaagagaa
     3361 gtccgtggac acccggtccc cagtatttct ccgcacgcga cggcgcttac ggctggaacg
     3421 atgcgctgaa accggaaacg gacgcttccg ctgttttttc atccgacaaa aaagccgctg
     3481 aagaaatgcg cgggctcggt ttcggaacgg tgatggtgta cagcaatgac ggcatcgcac
     3541 ggggaacagc atcctgcgtc acactgaacg cggaagacgg cgacaattcc gttttcgtga
     3601 aacagaaagt ggcgaattgc ctttccttcg acaaaggaac ctcgcggcag gaatatcctt
     3661 cctcgctcat gggagcgatc gcgctgctgc gccagaccta ttatgacgcc gactggtacc
     3721 gcacggcgaa aaaccggaaa gagttcaacc tttccctcga agcgtggaac aacgtgcagt
     3781 cgctgccgca gatcttcgag acgaccgaca aatggaacgt cctgcgtgca gacaaggtgg
     3841 gcgatgaatt caacatccag tatattttca aaggaagcgg caatgaatac cagcgcatga
     3901 acgaaatgaa gaacacgaat gcctcgttca tcctcccgct gaatttcccg aaagcattcg
     3961 acgtttccga tccgtatgat gcggaacaga tcgcatacac ggacctcaag cattgggaaa
     4021 tggcgccgct gaatcctggc gcatttgaaa aatacgggat caatttcgcg ctgacgactt
     4081 ccgatctcga agacaaaaaa gatttctggg aaaacctccg caaagcgatc aaattcggct
     4141 tgtcggaaaa agcggccctg aaagccctca caaccgttcc tgcgcaattg ctgggcatcg
     4201 cggataaaac aggcgcgctg aaaaaaggcc tgctggcgaa tttcatcatc acgaacggga
     4261 atgtgtttac cgatgatgcg gtgatctggg aaaactgggt gaaagggaag caatacatcc
     4321 tgcgcgatgc gaacaccgtc gacgtacgcg gaacatattc catgaccgtc ggcggaaacc
     4381 cgatgacact cgacgtggaa ggagacattt tcaagccgag aggatcgttc aaaaagatcg
     4441 acagcatcaa aggccccgtg aatgtttcct gcgacggcaa ccgcgtcgat atttcatgga
     4501 cgtataaaaa atcaggaacg accgtgctgc ttaccggcct catcgatccc aaaacaaaag
     4561 cgatgagcgg aagcggtcag gaacccgacg gcacatggat caaatggtcg gccacgcaaa
     4621 ccgcgccgtt cacaccgaaa ccggaaccga aagataccac cgccccggat atcccttcgc
     4681 tcgacgacgt gatatacccg ttcgcgcctt tcgggagaac gatcaaggat tcgctcgaga
     4741 tcgaccgttt ccagatg
//
LOCUS       DASTSF010000262         6038 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1178587,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000262 DASTSF010000000
VERSION     DASTSF010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 6038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6038
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1178587"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(238..957)
                     /locus_tag="VFU15_07570"
     CDS             complement(238..957)
                     /locus_tag="VFU15_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005842277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEU4717675.1"
                     /translation="MANNAFAFKKFTIHQDRCAMKVGTDAVLLGAWAKTYGVRNALDI
                     GTGTGVIALMLAQKCGAHIDAIDIDSDAVADAEENAAASPWADRVAVHHLSLQQFTET
                     AEKKYELIVSNPPFFSDSLPASEASRNAARHTVLLPFSDLIASVTKLLARDGKFCVIL
                     PSREGEQFRALAESKGLHLTRLTRVRTTPEKAEKRWLMQFAFMQKHISDSTLVIEKDN
                     LNAQHYTDEYRELTKEYYLYF"
     gene            complement(1110..1700)
                     /gene="rimM"
                     /locus_tag="VFU15_07575"
     CDS             complement(1110..1700)
                     /gene="rimM"
                     /locus_tag="VFU15_07575"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEU4717676.1"
                     /translation="MEKSSCFQFGRITRTSGVKGEIVIDLDVDRPGRYSAIDSFFAEI
                     NGSLVPLFVTKARLQGKQVIVQLDGFSSPSESETLVGCDAYLQLSFLPPLGKNKFYFH
                     EIPGFGVYDETYGNIGIAEDVVERSGQPVLRVMKDNIEILLPLFQGAVKNVDREKKTI
                     LVASPPGLIDIYLGKNESEQDGEADGNDFPEEEQPR"
     gene            1854..3263
                     /locus_tag="VFU15_07580"
     CDS             1854..3263
                     /locus_tag="VFU15_07580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717677.1"
                     /translation="MTFRKLLPALAFVACSFVSGSGIVRQDFDYDDWGTQPLDLGKNG
                     VLIEGHKGSYTRSGKSLLMARIDINGKLAWNKEIVIPNAKKSEIDYECASNDGNSIYV
                     LSFTEEKKKGGGQNPVITRVDTSGNSTSKSIDVEDFGFVITMYANSKYLFVITAIKDP
                     GWEDKLGNDVYKLYRFSKNDLSIEDLGDNDLCSVAKDDETFWQVFRVNDDYMEAFKVL
                     STDDHAIAVELGRFDEKGKLTTSKDIDIKLEKSFTRWNNSGCDAFPGVMNWRPAFTTL
                     AGGPNGVVYTKIRDGISRCHVIYDEINKCYLIFGITGPKAQVRIGAVFNGFFIQEVGD
                     DYTPGKMKEFSGFSDIDDDKLFNINSVPEGRYMDVYMSPGHHISVSLHGGKQPHSYYI
                     DENELTLTQNVGQWILPGGDWCPAPGDYINYATEYTSVDTKRKAFVDCIRTLYGQLTI
                     FDFWNDHKIQTLWEANNSK"
     gene            3500..4072
                     /locus_tag="VFU15_07585"
     CDS             3500..4072
                     /locus_tag="VFU15_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015029932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEU4717678.1"
                     /translation="MEDESFINELQSGDSGAFRKLLKLYRSLVYNTCLGMLQHAEDAE
                     DMTQEVFIEVYRSVKNFRMESKMSTWLYRVATNKCLDHLRSKQRGKRSGETVRIDGEV
                     NVQIPAGHNFSHPGVALEQKERAKILFAAISCLPENQKVAFTLAKVEGLSYQEICEVM
                     QMKLPAVESLVHRAKQNLKNLLKNYYQSEK"
     gene            4076..4399
                     /locus_tag="VFU15_07590"
     CDS             4076..4399
                     /locus_tag="VFU15_07590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717679.1"
                     /translation="MEKRSFNPEEEAELTLKSLDGISRADAPGALDEKIISSIESMRR
                     GKMRPAKWLWVAGIALMLINGAVAFIYLSPSGENQENTAVSAQQSSTISDVGSYYFSE
                     GGAWY"
     gene            4434..5072
                     /locus_tag="VFU15_07595"
     CDS             4434..5072
                     /locus_tag="VFU15_07595"
                     /inference="COORDINATES: protein motif:HMM:NF025173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HEU4717680.1"
                     /translation="MKLLENTKFTGAALIILLLMNTLLLGILVVKRPGRMMNRHEMRM
                     PGGPPPPGPDMQDHGPGPSAFLVQELGFSPEQQQQFEALMYRHRDAVQHYELLIHATR
                     DSLVHQLESEHPDSVLIARLSHNVGDYQSKIETATFDHFLAVRAICNDEQKEKFSSII
                     AEALRMMQPHGGPPMNGGPGMNGPPPPGNNGPAMNNNNPGMNGDRPFRNKSR"
     gene            complement(5256..>6038)
                     /locus_tag="VFU15_07600"
     CDS             complement(5256..>6038)
                     /locus_tag="VFU15_07600"
                     /inference="COORDINATES: protein motif:HMM:NF026121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4476 domain-containing protein"
                     /protein_id="HEU4717681.1"
                     /translation="GQSVADITPQGAPAAGQVVYVFNPSGDATPYSTTTTTTSGTQGT
                     GGSVTMNVSTSGDPNAGNMGTSTTVNDGSGQASTTTTTTSTTTTGTTDNANVGMNMGG
                     VGLGININVNDGTGGTGTTTTTTTTTTYSSTTTTSGTVDNGGTTTTTAVPPSTTSTST
                     SCSFPMSSADFEKAKNSISAQGFDESREKIAKQVLNTNCMSTAQVKEIMALFSFEETK
                     LDWAKFAYGKTTDPQNYYQLNDGFSFSSSVDDLNSYIESHQK"
BASE COUNT         1367 a         1589 c         1637 g         1445 t
ORIGIN      
        1 cgcaatgaac tttttgaatc ccatcccgtg aaagtttagg taaagttacg gaagaactgt
       61 ggcttccgct ttttgaagcg caccgttacc gcttctttgt tcctgtgttc cggtgctccg
      121 ctgcaagtcc tcgtgcctgc gggctttccg cttcgccccg ggctaccgtg gaacgttcgg
      181 ctcttttcag ttcctgtgcc gtatcgtaaa ctgcgaacgg gaatgagctt ccgctgatca
      241 gaaatagaga tagtattctt tcgtcagctc gcggtattcg tcggtgtaat gctgcgcgtt
      301 caggttgtcc ttttcaatta cgagcgtgga atcggaaatg tgtttttgca taaatgcaaa
      361 ctgcatgagc cagcgcttct ccgctttctc gggcgtcgtg cgcacgcggg tgaggcgcgt
      421 aagatgaagt cccttgcttt ccgccagcgc gcggaactgt tcgccctcgc gcgaaggaag
      481 gatcacgcag aatttcccgt ctcgcgcaag cagcttcgtc acggaagcga tgagatcgga
      541 aaagggcaga agcactgtgt ggcgcgcggc gttgcgcgat gcttccgaag ccggcagcga
      601 atcactgaag aaaggcgggt tgctgacaat gagctcgtat tttttttccg ccgtttccgt
      661 gaattgctgc agcgacaaat ggtgcaccgc cacgcggtcg gcccacggac ttgcggctgc
      721 gttctcttcc gcatcggcca ccgcgtcgga gtcgatatcg atcgcgtcga tgtgcgcccc
      781 gcatttctgc gcgagcataa gcgcgatcac gcccgtgccc gtgccgatgt caagcgcgtt
      841 gcgcacaccg tacgttttgg cccatgcgcc gagcagaact gcatcggtgc cgactttcat
      901 cgcgcacctg tcctggtgga tcgtgaattt tttgaaggca aaagcgttgt tcgccataaa
      961 cccggacggg atccccggaa gaattccggg ttaattatct aaaataagaa atttccggtt
     1021 cacttcggcc gcttcctgat gcctcacttt ccaatttccc gcccggaggc ttggagatac
     1081 ggaggttttt catcccccgt gcgcatctgt catcgcggtt gttcttcctc cggaaaatca
     1141 tttccgtccg cttcgccatc ctgttcgctt tcgtttttac cgagatagat atcgatgagt
     1201 cccgggggac ttgccaccag gatcgttttc ttttcgcggt ccacgttctt cacggcgccc
     1261 tggaaaagcg ggaggagaat ttctatgttg tccttcatca cacgcagcac gggctgcccg
     1321 cttcgttcca caacgtcttc cgcaatgccg atgttcccgt acgtttcatc gtaaaccccg
     1381 aacccgggaa tttcatggaa atagaatttg ttttttccca gcggcgggag gaacgacagc
     1441 tggagataag catcacagcc caccagtgtt tccgattccg aaggcgaact gaacccgtcg
     1501 agctgcacga tcacctgctt tccctgcaag cgcgccttcg tcacgaagag cggcaccagc
     1561 gaaccgttga tctcggcaaa gaaagaatcg attgcggaat aacgtcccgg ccggtcgacg
     1621 tccaggtcga tcacgatctc tccttttacg cccgaggtcc gggttatcct tccgaattga
     1681 aaacagctgc ttttttccat cggttaaaaa tactttgaaa gtcgttcgcg aagaaacggc
     1741 acatggaaag ccaggggaaa agaaggtgtt ttgtcagcca aatgttaact tattatcttc
     1801 tttctttgcg tatttccgga acattttaac tttataaacc aaatcctgtc cagatgacat
     1861 tcagaaagct cctacccgca ttggcctttg ttgcctgctc ttttgtttcc ggttccggta
     1921 tcgtccgcca ggatttcgac tatgatgact ggggaacgca acccctggat ctcgggaaaa
     1981 acggcgttct tatcgaaggc cacaaaggca gctatacgcg cagcggcaaa tcattgctta
     2041 tggccaggat agatatcaac ggaaagctgg cctggaacaa ggagatcgtt attcccaacg
     2101 ccaaaaaaag cgagatcgat tacgaatgcg caagcaatga cgggaacagt atttatgtgc
     2161 tttcgttcac cgaggaaaaa aagaagggcg gcggacagaa cccggttatt acacgggtag
     2221 acacaagcgg gaattccact tcgaaatcca ttgacgtgga ggatttcggt ttcgtgataa
     2281 cgatgtacgc gaattcaaaa tacctgttcg tgatcactgc catcaaggac ccgggctggg
     2341 aggataagct cggtaacgat gtgtacaaac tttatcgctt cagcaaaaat gatctttcga
     2401 tagaagattt gggcgataac gatctttgtt cagttgcgaa ggacgatgaa acgttctggc
     2461 aggtattcag ggtgaatgac gactacatgg aggccttcaa ggtgctttcc acggacgacc
     2521 acgcgatagc tgtggagctc ggccgttttg acgagaaggg aaagctgact acttcgaaag
     2581 acatcgatat aaaactggaa aaaagcttta cccgctggaa caatagtggt tgtgacgcat
     2641 tccccggcgt aatgaactgg cggccggcct ttaccacgct ggcgggcgga ccgaatggtg
     2701 ttgtgtatac gaaaatccgt gacggcatca gccgctgcca cgttatttac gacgagatca
     2761 acaaatgcta tctcattttc ggcattacag gtccaaaggc gcaggtaaga ataggtgctg
     2821 ttttcaacgg ctttttcatc caggaagtag gtgatgatta cacgccggga aaaatgaagg
     2881 agttttccgg tttcagcgat atcgatgacg acaaactctt caacatcaac tcggtcccgg
     2941 aaggaagata catggatgtt tacatgagtc ccggccatca catttcggtt tcccttcatg
     3001 gcgggaagca gcctcattcc tattacatcg acgaaaacga gctgacactg actcaaaacg
     3061 tgggccagtg gatattgccg ggcggcgact ggtgcccggc cccgggtgat tacatcaatt
     3121 atgctaccga atataccagc gtcgatacaa aacggaaagc cttcgtagat tgtatccgca
     3181 cgttgtacgg ccagctaacc attttcgatt tctggaacga ccacaaaata cagacgcttt
     3241 gggaagccaa taattcgaaa taaggcggcg ctccagtacg ggctcacgcc tgtattcagc
     3301 ggatctttgc ctctgcggta caaaacgggc tgcaataagc tgaataccgc agtttggcag
     3361 gtaaaatcca ataccggtac cagcataacg ttccggtttt tctttggttt ttttggcatc
     3421 cgcgatggca ggggccgaaa gtttcttcct cctatgacgt cttatcacta aagattagct
     3481 ttggtcccgc atacctggga tggaagacga atctttcata aacgaactgc agtcgggtga
     3541 ttccggcgct ttcagaaagc tgctcaagct ctaccgcagc ctggtgtaca acacctgcct
     3601 gggaatgctg cagcacgcgg aagatgcgga ggacatgacg caggaggtgt ttatcgaagt
     3661 gtatcgttct gtgaaaaatt tcaggatgga atcgaaaatg tccacgtggc tgtaccgtgt
     3721 cgcgacgaac aaatgcctcg atcatctgcg ttcgaagcag cggggaaaac gctccggcga
     3781 aacggtccgc atcgacggcg aggtaaatgt gcagataccg gccggccaca acttttcgca
     3841 tcccggcgtt gcgctcgagc agaaggaaag ggcgaagata cttttcgctg ccatctcctg
     3901 ccttccggaa aaccagaagg tcgcgttcac cctcgcaaaa gtggaagggc tgagctacca
     3961 ggagatctgc gaagtgatgc agatgaaact gcccgctgtc gaatcgctcg tgcaccgtgc
     4021 gaagcagaac ctgaaaaatt tgctgaagaa ctattaccaa tcggaaaagt aggtcatgga
     4081 aaaaagatca ttcaacccgg aagaagaagc ggagctcacc ctgaaaagcc tcgacgggat
     4141 atcgcgtgcg gatgcgcccg gtgcgctgga tgaaaaaata atttcttcca ttgaatcgat
     4201 gcgccgggga aaaatgcgcc ccgcgaaatg gctctgggtg gcgggcattg cgcttatgct
     4261 gatcaacggg gccgtcgcgt tcatttacct cagtccttcc ggtgaaaacc aggaaaacac
     4321 agccgtttcc gcacagcaat catccacgat cagcgatgtg ggttcgtatt atttttccga
     4381 aggcggcgcg tggtattgaa atacaactgt tgaattgtca aaataactga gtcatgaagc
     4441 ttctcgagaa cacaaaattt accggcgccg ccctcatcat tcttttgctg atgaacaccc
     4501 tgctgctcgg gatccttgtg gtcaagcggc cggggaggat gatgaaccgc catgaaatga
     4561 gaatgccggg cggtccgccg cctcccggac ccgacatgca ggaccacgga cccggcccgt
     4621 cggctttcct cgtgcaggag ctcgggtttt cgcctgagca gcagcagcag ttcgaagcgt
     4681 tgatgtacag gcaccgcgat gccgtgcagc attacgagct tctcattcac gccacgcgcg
     4741 attcgctggt gcaccagctg gaaagcgagc atcccgattc ggtgctgatc gcacggcttt
     4801 cacataacgt gggcgattac cagtcgaaga tcgaaaccgc cacgttcgat cattttctcg
     4861 ccgtgcgcgc catctgcaac gacgagcaga aagaaaaatt ctcctccatt attgccgagg
     4921 cgttacgcat gatgcagcct cacggtggtc ctcccatgaa cggaggtccc ggcatgaacg
     4981 gtccgccgcc cccgggcaat aacggtcccg cgatgaataa taacaacccg ggaatgaacg
     5041 gcgaccggcc gttccgcaac aagagccgtt agttttcatc gtccgtcaca tccctggtcc
     5101 tgcactccgt gcaggatttt tttatccggg atgcggaagg cgcatcctgc ctggcgaacg
     5161 gctttcacat gaccgggccg taaatgaaaa atggccatcc cggaaagcaa aaaagccgca
     5221 gaaaatttct gcggctttcc atttagtaag cgcggttact tttggtggct ttcgatgtag
     5281 ctgttgaggt catccacgga agatgagaat gaaaatccgt cgttcagctg gtaatagttc
     5341 tgcggatcgg tggtttttcc gtatgcgaac ttcgcccagt cgagcttggt ttcctcgaag
     5401 gaaaacagcg ccatgatctc tttcacctgc gccgtgctca tgcagttcgt attcagcacc
     5461 tgcttcgcga tcttctcgcg gctctcatcg aatccctgtg cggagatcga gtttttcgct
     5521 ttttcgaaat cagcggaaga catcgggaaa gagcaggatg ttgacgtgga ggtcgtgctt
     5581 gggggaacgg cggtggtggt ggtggttcct ccgttgtcga ctgtgccgct ggttgtggtg
     5641 gtgctggaat aggtggtcgt cgtcgtcgtg gttgtcgtcg ttccggttcc gccggttccg
     5701 tcgttcacgt tgatgttgat gccgagaccg acaccgccca tgttcattcc cacgttcgca
     5761 ttgtcggttg tgcccgtggt cgtggtggac gtggtcgtcg tcgtggtcga tgcctgcccg
     5821 cttccgtcgt tcaccgtcgt gctggtgccc atgttgcccg cgttcggatc tccgcttgtg
     5881 ctgacgttca tcgtaaccga tccacccgtg ccctgtgtgc cggaagtggt cgtcgtcgtc
     5941 gtggaataag gcgtcgcatc gccggaagga ttgaacacgt atacgacctg gcccgctgca
     6001 ggagcgccct gcggcgtgat gtcggcaacg ctctgtcc
//
LOCUS       DASTSF010000263         2062 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1909532,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000263 DASTSF010000000
VERSION     DASTSF010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1909532"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1359
                     /locus_tag="VFU15_07605"
     CDS             <1..1359
                     /locus_tag="VFU15_07605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU4717682.1"
                     /translation="EEQADFFTTKDREVLGKGVLVDIPEEKIQAAAGDERWLHTKKIP
                     LRGSDGKPAFLLGISEDITEKRAREEELRRSNDRFDKILKMSPTAIFVSEVETGFFLY
                     VNETFCRLFQVSGKEVAGKTSVDLGISTPERRAEITQNMMRNNGRVRGLEFRYEPAHG
                     EAADLLMSSEIVEFEGKKCFLGSISDITERKRSEEKIARLNDELEESLRQLGTVNKEL
                     EAFTYSVSHDLRAPLRAVSGYANMLEEEYNQLLDEEGRRLLGVIRYNAKKMGQLIDDL
                     LAFSRLGRKEIETRPENMQTLVEGVMIELRKTIDYKAKITIGELHTANVDYGLMHQVF
                     LNLISNAIKYSSKKKKPEISISSEVREGEIIYSIRDNGVGFDMRYYDKLFGVFQRLHK
                     MEEFEGTGVGLAIVQRIISKHRGRVWAEAAVNKGANFSFSLPAEKPASLTQTAYGRNQ
                     EH"
     gene            1337..1780
                     /locus_tag="VFU15_07610"
     CDS             1337..1780
                     /locus_tag="VFU15_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013295660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU4717683.1"
                     /translation="MAETRNIEILLVEDNPNDAEMTIRALKKNNLANNLYHVSDGEEA
                     LDFIFGRGQFSDRARYNSPKLVILDLKLPKVDGLEVLKEIKENAETKTIPVVIMTSSR
                     EERDLVDGYKLGVNSYVVKPVEFGDFMKAVTQLGLYWMLQNVTAG"
     gene            1830..>2062
                     /locus_tag="VFU15_07615"
     CDS             1830..>2062
                     /locus_tag="VFU15_07615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU4717684.1"
                     /translation="MKILILEDNPADIEFIRRELKKAGKNFVVSITDTREEFVRAVKE
                     FDPEVIISDHSLPQFNSLEALNIAKEQVPWAAFL"
BASE COUNT          545 a          537 c          579 g          401 t
ORIGIN      
        1 gaggagcagg cggatttttt caccaccaaa gacagggaag tgctgggcaa gggtgttctc
       61 gttgacattc ccgaagaaaa aatacaggcg gccgccggcg atgagcgatg gcttcacacg
      121 aaaaaaattc ccctgcgggg aagcgacggg aaaccggctt tcctgctcgg catatcggaa
      181 gacatcacgg agaaaagagc gagagaagaa gagctgcgcc ggtcgaacga ccgcttcgat
      241 aagattctca agatgagccc gacggccatt ttcgtttccg aagtggaaac cggttttttc
      301 ctttatgtca acgaaacgtt ctgccgcctt ttccaggtca gcggcaagga agtggccggc
      361 aaaacctcgg tcgacctcgg catatcgaca cccgaacgcc gcgcggagat cacgcagaac
      421 atgatgcgca ataacggccg cgtgcgcggg ctggaattca ggtatgaacc cgcgcatggc
      481 gaagcggccg acctgctgat gtcgtcggag atcgtggagt tcgagggaaa gaaatgtttc
      541 ctgggaagca tttcggacat caccgaacgc aaacgttccg aagaaaagat cgcacggctc
      601 aacgatgagc tggaagaaag cctgcggcag ctcggaacgg tgaacaaaga gctcgaggcg
      661 ttcacgtatt ccgtttcaca cgacctgcgt gcgcccctgc gcgccgtgag cgggtatgcg
      721 aacatgctcg aggaagaata caaccagttg ctcgacgaag aaggccggcg gctgctcggc
      781 gtgatccgct acaacgcaaa aaaaatgggg cagctgatcg acgaccttct tgcattttcg
      841 cggctgggaa gaaaagagat cgaaacgcgc ccggaaaata tgcagaccct ggtggaaggc
      901 gtgatgatcg agctgcgcaa aaccatcgat tacaaggcga agatcacgat cggcgaactg
      961 cacacggcga atgtcgatta cggtcttatg caccaggttt tcctgaacct tatttcgaac
     1021 gcgatcaaat actcttccaa aaagaaaaaa ccggaaatat cgatcagctc cgaagtgcgt
     1081 gaaggagaga tcatttattc catacgcgac aacggcgtcg gtttcgacat gcgctattac
     1141 gacaagctgt tcggcgtgtt ccagcgtctt cacaaaatgg aagagttcga aggcacgggc
     1201 gtgggcctcg cgatcgtgca gcgcatcatc agcaagcacc gcggccgcgt atgggcggaa
     1261 gcggcggtga acaaaggcgc gaatttttct ttctcactcc cggcagaaaa accggcttct
     1321 ttaactcaaa ccgcttatgg cagaaaccag gaacattgag atccttcttg tcgaagacaa
     1381 tcccaacgac gcggaaatga cgatccgcgc gctgaaaaaa aacaacctcg cgaacaacct
     1441 ctaccacgtt tccgacggcg aagaggcgct tgatttcatt ttcggacgcg ggcagttttc
     1501 cgaccgcgcc cggtacaatt ctcccaagct cgtcattctc gatctcaagc tcccgaaggt
     1561 ggacggcctc gaggtgctga aggagatcaa ggaaaatgca gagacgaaaa ccattcccgt
     1621 ggtgatcatg acctcctcgc gggaagagcg cgaccttgtc gacgggtaca agctgggtgt
     1681 gaacagctac gttgtaaaac cggtcgagtt cggggatttt atgaaagcag tcacgcagct
     1741 gggtctgtac tggatgctgc agaatgtcac agcgggctga agcccgcact ttagttgccc
     1801 aaaccaagaa cagcatgaat actgagccgt tgaagatcct catcctcgag gacaatcctg
     1861 ccgatattga gttcatccgg cgtgagctga aaaaggcggg gaagaatttc gtcgtttcca
     1921 tcacagatac gcgcgaagaa tttgtccgtg ccgtgaaaga attcgatccg gaagtgatca
     1981 tctccgatca ttcccttccg cagttcaatt cgctcgaagc gctgaacatt gcgaaagagc
     2041 aagttccgtg ggccgcgttc ct
//
LOCUS       DASTSF010000264        13085 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_524320,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000264 DASTSF010000000
VERSION     DASTSF010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 13085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13085
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_524320"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..743)
                     /locus_tag="VFU15_07620"
     CDS             complement(<1..743)
                     /locus_tag="VFU15_07620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717685.1"
                     /translation="MKFRFSIGRKILLGFAALILLVLIAFSLTLRTIRQSREINDTIA
                     KLYTPSVDSLQEMRLLITNSKALINNWISVPGPSDDKPKLKILLDQEYPQLKKRITHL
                     SLGWSDTDRVLVDSIFVDCEALWVMHRNVMNNLSDVKSYNDPGIMFDVQSSVLDNDGV
                     IVVQTKHILARLDKLKGLVNIAAAQKRDEMLASFQRLQFVVVLLLIVLPVGGLVIALL
                     TARTITRPVHYIKNILLSMAKGILPNQPIT"
     gene            complement(765..1688)
                     /locus_tag="VFU15_07625"
     CDS             complement(765..1688)
                     /locus_tag="VFU15_07625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717686.1"
                     /translation="MKRIVRIHVILLLGVILLGGRTLHAQTAMYYNATLHFLQVGQVD
                     SAKADVEKLMAAPGAKSDPDNWYLYGVVLKEMYKTYERNNFDSPYRKQSVDAFKTSLK
                     LDTVADRKKVTRDNLRFIAGTFYNDAVTTLDTSRYKTSIQCYEQYREVTLIVDSTFDI
                     KKKDIEFYLALASTYNTIYNLDKKKNAAFFDLTRDTYLKVLTWDPNNYMANYSMSLLY
                     WNKGVDLMYNIDYDDSLTDVFGVQDHSVELFKQSLPFAEKAYTMEPNREETLIVLSGI
                     YYSLNEFEKSKQYQQMLDDLRKRNPGDGQQR"
     gene            complement(1695..2228)
                     /locus_tag="VFU15_07630"
     CDS             complement(1695..2228)
                     /locus_tag="VFU15_07630"
                     /inference="COORDINATES: protein motif:HMM:NF025069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HEU4717687.1"
                     /translation="MKRFLALLALISLPVLFSFQAPATPAGDTNARIKSVFIYNFTRY
                     IEWPADYKSGNFIINMFGTNAAMLSELNNMAKTKTVGTQKIEIHNTTTLDGIGKCNIL
                     YVTPDEATPLTDIIARLKGKSTLLVTEKPGLAKQGSAINFVIVDNRQKFELNQSNAEK
                     YNLTVSSALVALAIPVQ"
     gene            2424..2927
                     /locus_tag="VFU15_07635"
     CDS             2424..2927
                     /locus_tag="VFU15_07635"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HEU4717688.1"
                     /translation="MLKRITPVLFLLLVACHGQKHVASSDSDNALRKKYAGILQVDEK
                     EITNLRLYRFIDEWYGVPYKYGGKTKAGIDCSGLTCTLYSEVYGKTISGSAASLNDQC
                     EEVKEKNLAEGDLVFFKIGSDKVSHVGVYLQNRHFVHASTHKGVMISSLDEAYYQKYF
                     FRGGKLK"
     gene            complement(2943..8462)
                     /locus_tag="VFU15_07640"
     CDS             complement(2943..8462)
                     /locus_tag="VFU15_07640"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HEU4717689.1"
                     /translation="MKRFNVFITILFTLSLSVARAQQGINGSLTLSSGTTIVNEYTAI
                     TGYVPAGSTSITVANSNMNANGRFGWNLRPGDLVMIYQPQGVWLRDGWSVANQSDTSW
                     GAIVNPWDYNSAGRYELHQVVSVPNLTTINIDCGTTYTYYHNGDLGGEACNPEVIRVP
                     RYINLTINAGATLSCDDWNGTIGGILAVETSGDITVNGTIDATGKGFRGGALNSDNAT
                     SYGSAVVFNSDSTLGAARGEGVAGYMRQYNTYGGAFGGSYCRGAAANAGGGGDGHNAG
                     GGGGANAPNGSNPGALWSGNGIPDQSGAGWTTAWNLEPPVNTMSLRTAANSAGGGRGG
                     YSFSSNNRNATTTAPGNALWGGDSRNPQATGLGGRPLDYSGGRLFMGGGGGAGDQNNG
                     AGGNGGDGGGLIYLMSFGNINGTGTIVANGNDGGTANGIDAAGGGGAGGTIVLNAVST
                     VANTLTVNANGGNGGNQNVSLTTEAEGPGGGGGGGYIAVSSGAPVRNANGGNNGTTNS
                     LGLTEFLPNGATKGCPGTSNGSITNFTISATNVTICAGGSTTLTATLSGTVPGGTTII
                     WYDAATAGTQLGTGSTYTTPALGTTTTYYVTTCPGFWRIPVTVTVGPPPTVTASASSP
                     GICTGGSTTLTASGATTYTWMPGNLSGSSVTVTPGATTTYTVTGSNGPGCSNTASVTV
                     TVTPPPTLTVSATNPTICNGSSTTLNASGATSYTWMPGNLSGSSVTVSPTSNTTYTVT
                     GTTNGCSSNAQVTVTVNPTPTVTATAASPTICAGSSTSLTGSGATSYTWMPGSLSGSP
                     VTVTPASTTTYTVTGTDGNGCSSTGQVTVTVNPLPVVSVSPASTTICSGNNTTLTASG
                     GTTYTWMPGSLSGSSVSVSPSITTTYTVTGTTSGCTNTASATVTVNPTPTVTATASAS
                     PICIGSPSTLTASGATTYTWMPGNLSGSSVTVTPATTTTYTVTGTSAGCTSTAQVTVT
                     VNPSLVVTATASSSTLCDGTPDTLTSSGATNYTWNPGNLTGASVIVTPSSSVTYTVTG
                     DDGNGCTSTAQVSVTVNPSPTVTVTPSGATICSGSSTTLTASGGTTYTWMPGNLSGAS
                     VSVSPSSTTTFTVTGTSSGCSDTAQSVITVNATPAANAGNDTAFCSGGSVTITATGGG
                     TYVWNGGTLSNASGASQVVSPSSVTTYTVDVTAANGCSASDSVVVSVNALPAANAGTD
                     QVVCSGTVVNLSATGGGSYSWNGGSLVNAGGQNQSDAPSSTTNYVVTVTDPNGCSAND
                     TVLVSVNALPNISAGPDLSVCPNSTAQLSVSGGTLYTWMPGSTLSDSTISNPVASPTV
                     STTYVVTGTDANGCSASDTVTVNVSSVLSVNAGPDVSICQGDTIQLSTSGGTTYVWSP
                     ASSLTSPNSDTTGAFPASTTTYVVNVTDANNCSGSDSVTVFVNPPVTLTTAGATTICI
                     GQSATISATPSGGNAPYTFTWSNSLTGPGPQTVSPVTTTTYSVSVTDSLGCSSTQQTV
                     TVNVNPALSASSASPPAACSGTPVTLNASATGGDGNYSYTWLPSSQSGSSLTFTPSST
                     TTVSVVVTDGCGTPADTTPVAVTVNALPVVTAQASFYEGCGYLCVDFTGQSTGNCASS
                     SWTFGDSDSSNVQNPSHCYSVGTYDVSYTCTDNNGCTGTATMPAMITVYPPPTAAFTP
                     SITSSVLLLPQGSSQQICFADNSTGATSWSWAFGTDTSTQQSPCFTIADTGMYCARLA
                     VSSQFSCPDTARYCFDVVTDANFSIPNVFTPNGDGVNDMFMITGTDIKGFRCEIYDRW
                     GVKIYEWNSVGGGWDGKTTSGADAVDGVYYYVMSITDYADNTKNRQGFVQLIRGK"
     gene            complement(8551..9756)
                     /locus_tag="VFU15_07645"
     CDS             complement(8551..9756)
                     /locus_tag="VFU15_07645"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU4717690.1"
                     /translation="MPRGLSAEFFNSVSGFSSREWNSVAAGSSLFLSAEFLSAFEQAA
                     DPNVSFWYAVIREKQQAVGVVCFQVITLAADEIVKILKPAVSAAGFLGSGTTFSGIVG
                     KLREEKGMRILICGNNFISGEYCVAVKKEYDAEKIYRAIPPVVKYITGHDRSRAKISA
                     ILVKDFFEKKKEHPSSSLKKKRYHRFMVEPEMVVDIRSEWKTFGDYLESMSKKYRNRA
                     KKVMKDTETVVCEEWGIEKIAAHEEKIYSLYMNVHARAKFRLAPLGKNYFSAMKNAFP
                     GKFRLHVYLVGGEPVAFRSSFLLKSHLEAHFIGLDYDANERNPLYQRILYDFVNEGIT
                     SPVPSVYLGRTASEIKSTVGAVAHDLVCYIRHRNSLSNQLIRPFIEYLKPSEWIPRNP
                     FKAETVGTD"
     gene            complement(9918..10886)
                     /gene="pfkA"
                     /locus_tag="VFU15_07650"
     CDS             complement(9918..10886)
                     /gene="pfkA"
                     /locus_tag="VFU15_07650"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEU4717691.1"
                     /translation="MKKIAVFTSGGDAPGMNACLRAVVRTAVAEGLEVLGIRYGYEGM
                     IDGDFFGMDASSVANIIQRGGTILKTARSKRFLAPEGRAQAKAQVEKHGIEGMIAIGG
                     DGTFRGAVEFSKICSVPVIGCPGTIDNDLNGTDYTIGFDTAINTVIDAVDKIRDTAES
                     HNRVFIVEVMGRDAGLIALYSGIGAGAEGILIPESRNDMRNVMERLGSGREDKRSKII
                     IVAEGDEAGGAFRVSEEVKKRFPSLDVRVTILGHIQRGGSPSCMERVNASRIGYEAVK
                     ALIAGKRQLMTGIVSGKITFTPLELAVKQMQEPNPELLNMIDILAR"
     gene            complement(10938..12119)
                     /locus_tag="VFU15_07655"
     CDS             complement(10938..12119)
                     /locus_tag="VFU15_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006990256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HEU4717692.1"
                     /translation="MKPGEFRMVAKTMQGLENILAKELLRIGAREIEEHKRAVSFIGD
                     KGTMYKANYLLRTALRILKPVSSFHAKNEKDFYDGVYAIDWTQWMTPDDTLAVDCTLN
                     TEIFNHSYFIAQKTKDAIVDQFRDKFGRRPSVDTEFPALRINVHVSREEVNISLDSSG
                     YSLHKRGYREDTGRAPMNEVLAAGIVQLTEWNPRFRLIDPMCGSGTIAIEAALYANHI
                     PPGYFREKFGFEKWNDFDKELWEKITDAALDRISSEQQDITAFDISQNVLRKARENIL
                     RAKVEDVVKTFTADFFEWTPPEGKGVLIMNPPYGERMDKDDSDVFYKQIGDTFKKKYS
                     GYEAWLITSNMEAIKVIGLRPSRKITLFNGPLECKLLKFEMYAGTKKIHKLRKEDGPE
                     D"
     gene            12244..12705
                     /locus_tag="VFU15_07660"
     CDS             12244..12705
                     /locus_tag="VFU15_07660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717693.1"
                     /translation="MSNGLTAVIMDVICLAGSGLAETEHEKDLMIWFAQKDQDIFGIG
                     IVGFDVSDMGWKKEIFEAQKSFILEVIDSAAQKTGWEKLNYSPKEDRVLPVLKELREM
                     IMAFSETHAENAVPLKIYPFEEKIMKYDRCEKHRVYLHTLGCIVCNNAALG"
     gene            complement(12721..>13085)
                     /locus_tag="VFU15_07665"
     CDS             complement(12721..>13085)
                     /locus_tag="VFU15_07665"
                     /inference="COORDINATES: protein motif:HMM:NF014582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HEU4717694.1"
                     /translation="SSDPKIFPPFLTGIVLHNIPIAIAFMGLLLHNGNSKMKSFLFLS
                     LFALMTPAGMIGSYVIGENLQGDLSPYFSAITGIVVGIFLHISTTILFESTENHRFNL
                     IKFVTILFGVAVAWAVIG"
BASE COUNT         2948 a         3476 c         3527 g         3134 t
ORIGIN      
        1 gtgatcggct ggttcggtaa aatgcctttc gccatcgaca gcaggatgtt cttgatgtaa
       61 tgcacgggcc gcgtgatcgt ccgcgcggtc agcaatgcga taacgagtcc gcccaccggc
      121 agcacgatca ggagcaggac gaccacgaac tgcagtcgct ggaacgaggc gagcatctcg
      181 tcgcgtttct gcgcggccgc tatgttgacg agccccttca gcttatcgag acgcgcaaga
      241 atgtgcttcg tctgcacgac gatcacaccg tcattatcaa gaacgctcga ctgaacgtcg
      301 aacatgatac ccgggtcatt gtagcttttg acgtccgaaa gattgttcat cacattccgg
      361 tgcatcaccc agagcgcttc gcaatcgacg aaaatggaat caacgagcac gcggtcggta
      421 tccgaccacc cgagagaaag atgcgtaatg cgttttttca gctgcgggta ttcctggtcg
      481 agaaggatct tcagcttggg tttgtcgtcg ctgggaccgg gaacgctgat ccagttgttg
      541 atgagcgctt tcgaatttgt gatgagcagg cgcatttcct gcagtgaatc gacggaaggt
      601 gtgtacagct tcgcgatggt atcgttgatc tcacggctct ggcggatcgt gcgaagcgtg
      661 agtgaaaacg cgatcaggac gagcagaata agcgcggcga aaccaagcag aattttacgc
      721 cctatcgaaa aacgaaattt cataaatctg tctggggtcg ctgtttatcg ctgttgtccg
      781 tctccgggat tacgttttct caggtcatcc agcatctgct ggtattgttt ggatttctca
      841 aactcgttga ggctgtaata aatcccggaa agaacgatga gcgtttcttc acggttgggc
      901 tccatcgtgt acgctttttc tgcgaacggg agcgactgtt tgaacaattc caccgaatgg
      961 tcctgtacgc cgaaaacgtc cgtgagactg tcgtcgtaat cgatgttgta catcaggtcg
     1021 acgcctttgt tccagtagag caggctcatg ctgtaattcg ccatgtaatt gttcggatcc
     1081 cacgtcagca ctttcaggta agtatcgcgt gtaaggtcga agaaagcggc gttctttttc
     1141 ttgtcgagat tgtagatcgt gttgtacgtg gatgcgagcg cgagataaaa ctcgatgtct
     1201 ttcttcttga tgtcgaacgt gctgtcgacg atgagcgtca cttcgcggta ttgctcatag
     1261 cattgaatgg acgttttgta acgcgacgta tcaagcgtag tgacggcgtc attgtagaaa
     1321 gttcctgcaa tgaaacgaag attgtcgcgt gtcactttct tgcggtcggc gaccgtatcc
     1381 agcttcaggg aagtcttgaa cgcatccacc gactgtttcc ggtaaggtga atcgaaattg
     1441 ttgcgctcat aggtcttgta catttccttg agaacgacac cgtagaggta ccagttgtcc
     1501 gggtccgatt tcgcgccggg agcagccata agtttttcca catctgcttt tgcgctgtcc
     1561 acctggccca cctgcaggaa atgaagcgtg gcattgtagt acatggccgt ctgcgcatgc
     1621 agcgtccttc cgccgaggag gatcaccccg aggagaagaa ttacgtgtat gcggactatt
     1681 cttttcatgt tcgtttattg tacaggtatg gccagcgcga cgagcgcgga gcttacggtc
     1741 aggttgtatt tttcagcatt tgactggttg agctcgaatt tctgccggtt atccacaatg
     1801 acaaaattga tcgcggatcc ctgcttggcc aggcccggtt tttcagtaac gagcagcgtg
     1861 ctcttccctt tcagccttgc gattatgtct gttaacggag ttgcttcgtc cggggttacg
     1921 taaaggatgt tacattttcc tattccgtcc agcgtggtgg tgttatggat ctcgatcttc
     1981 tgcgttccca ccgtcttggt tttggccatg ttgttcagct cggaaagcat ggccgcattg
     2041 gtgccgaaca tattgatgat gaaattcccg ctcttgtaat cggccggcca ctcgatataa
     2101 cgcgtgaagt tgtaaatgaa tacggattta atgcgcgcgt tcgtgtcgcc ggcaggagta
     2161 gcaggtgcct ggaagctgaa aagcaccggc agtgatatca aagccaataa cgcgaggaag
     2221 cgtttcataa atcggttaaa gcaaaggtca gaccaaatcc gtccattccc ggatatgatc
     2281 gtgctattct ttcaaaggta ggaattccct ctgaattctc aagacagctt tggttatcaa
     2341 catattccat gtgaaaagtg tgggcgcatc catccgcccg aagccatcgc cggcgctaaa
     2401 tttgaaagaa tgtgcggtga aagatgctga aaagaattac ccctgtcctg ttcctgctcc
     2461 ttgttgcctg ccacggacaa aagcatgttg cgtcctctga cagcgacaat gcgctgcgaa
     2521 aaaaatatgc aggcatcctc caggtcgatg aaaaggagat caccaacctc aggctttacc
     2581 gctttattga cgaatggtac ggcgttcctt acaaatacgg gggaaaaacc aaagcgggca
     2641 tcgattgttc cgggcttacc tgcactcttt acagcgaagt ttacggcaaa acgatctcgg
     2701 gttccgcagc ttcgctgaac gaccagtgtg aggaagtgaa ggagaaaaat cttgccgaag
     2761 gagatctcgt ttttttcaag atcggctcgg acaaagtttc acatgtgggc gtatatcttc
     2821 agaaccgcca tttcgtacat gcatcgacac acaagggtgt catgatcagc agcctggatg
     2881 aagcgtatta ccagaaatac tttttccgcg gcgggaagct gaagtaatta cagagagctc
     2941 cgctattttc cacggatcag ctgaacgaat ccctgccggt tcttcgtatt atcggcgtaa
     3001 tcggtgatgc tcatcacgta ataataaacg ccgtcgacgg catctgcccc ggaagttgtt
     3061 tttccgtccc atcctccgcc aactgaattc cattcgtaga tcttcacgcc ccagcggtca
     3121 tagatctcgc aacggaatcc tttgatgtcc gtcccggtga tcatgaacat atcgttgacg
     3181 ccgtcgccgt taggcgtaaa gacgttcgga attgaaaagt tggcatcggt gaccacatcg
     3241 aagcagtaac gcgccgtatc aggacaggag aactgcgagg aaacagcgag acgcgcgcag
     3301 tacattcccg tatcggcaat cgtaaagcac gggctctgct gcgtcgatgt atcagttcca
     3361 aatgcccagc tccaggaagt cgcaccggtg gaattatccg caaagcagat ctgctgtgaa
     3421 gatccctgcg gcagcagcag cacgctcgat gtgatcgaag gcgtgaaggc cgcagtcgga
     3481 ggcggataga ccgtgatcat cgccggcatc gtcgccgttc cggtacaacc gttgttatcg
     3541 gtgcaggtat acgaaacatc atacgttcct acggaatagc aatgcgaagg attctgcacg
     3601 ttgctcgaat cggaatcacc gaatgtccac gaggaagatg cgcagttgcc tgttgattgt
     3661 cctgtaaaat caacgcacag gtaaccgcat ccttcgtaga agcttgcctg cgctgtcacc
     3721 acgggcaatg cattaaccgt aacagccacg ggcgttgtgt ctgccggggt tccgcatccg
     3781 tccgtcacta caacgctgac ggttgttgtc gaagaaggcg tgaaggtgag cgaagaaccg
     3841 ctttgtgacg aaggcagcca cgtgtagctg taatttccgt caccgccggt ggcgcttgca
     3901 ttcagcgtga ccggtgtgcc gctgcatgca gccggcggcg aagcggatga agcgctcagc
     3961 gccgggttca cgttcaccgt tacagtctgc tgcgtggaag agcagccgag cgaatcagtg
     4021 acagaaacgg aatacgtcgt ggttgtaacc ggtgaaacgg tttgcggtcc gggtccggta
     4081 aggctgttgc tccacgtgaa cgtatatggc gcatttccac ccgacggcgt tgccgagatc
     4141 gtcgcggatt gcccgatgca gattgtcgtt gcacctgcgg tggttaacgt taccggcggg
     4201 ttgacaaaaa cagtgacgga atcgctgccc gaacagttat tggcgtccgt tacgttcacg
     4261 acgtacgtcg ttgtcgatgc gggaaacgcg cccgtggtat ccgaattcgg cgatgtcagt
     4321 gatgatgccg gcgaccacac ataagttgtt cctccggaag tggaaagctg aatggtgtcg
     4381 ccctggcaaa tggaaacatc cggaccggca ttaacagaaa gcacgctgga gacattcaca
     4441 gtcaccgtat cgctggccga gcagccgttc gcatcggttc ccgtaaccac gtacgttgtc
     4501 gaaaccgtcg gtgacgctac cggatttgaa atcgtggaat cgctgagcgt ggaaccgggc
     4561 atccaggtgt acaatgttcc gccggatacc gacagctggg ccgtgctgtt gggacaaacg
     4621 gaaagatcag gtccggcaga aatattcggc agcgcgttga cggaaaccag taccgtatca
     4681 tttgcggaac acccattggg atcagtgacg gtcacaacat aattggtcgt agatgacgga
     4741 gcatcggact gattctgccc gcctgcattc accagcgaac ctccgttcca ggaataagat
     4801 ccgccgcctg tcgcggaaag attcaccacc gttcccgagc agacgacctg gtcggttcct
     4861 gcattcgctg cgggtaatgc attcaccgaa acaacaacag aatcagaagc ggaacacccg
     4921 ttcgccgcag ttacatcaac cgtatatgtt gtgacggaag aaggcgaaac cacctggctg
     4981 gcgccggaag cattcgaaag cgttccgccg ttccacacat aagtgccgcc gccggttgcc
     5041 gttatggtta cagatccccc gctgcagaaa gccgtgtcat ttccggcatt ggcagcaggc
     5101 gtcgcattga cggttattac tgattgtgca gtgtcggaac agccggatga agttcccgta
     5161 actgtaaacg ttgtagtgga agaaggactt acacttaccg atgcgccgga taaattgccc
     5221 ggcatccacg tgtacgttgt tcccccggat gccgtaagcg tggtgctgct tccgctgcag
     5281 atcgttgcac cggaaggtgt aacggtaaca gtgggcgaag gatttacagt tacgctcacc
     5341 tgcgctgtgc tcgtgcagcc gttcccgtca tcgccggtaa cggtgtacgt aacggaagac
     5401 gaaggcgtga cgatcacaga agcgcccgtc aggttaccgg gattccaggt gtagttcgtc
     5461 gcaccggatg atgtcagcgt atcgggtgtg ccatcgcaga gcgtggatga agaagccgtt
     5521 gccgttacaa caagcgatgg attcacggtt acagtcacct gcgcggtgga tgtgcatccg
     5581 gcagaagttc ccgtcactgt ataagttgtc gtagtggcag gcgttaccgt taccgatgaa
     5641 cctgaaaggt ttcccggcat ccacgtatac gtagttgcac cgctcgctgt gagtgttgaa
     5701 ggagaaccta tgcagatggg actcgcggaa gccgttgcgg ttacagttgg cgtcgggttg
     5761 accgttacag tggcggaagc cgtattcgtg cagcccgatg tggttcccgt cacagtgtac
     5821 gtcgtcgtta ttgaaggcga aaccgaaacg gaagatcccg aaagacttcc cggcatccac
     5881 gtgtatgttg ttccgccgga cgccgtgagt gtcgtattgt tcccgctgca aatcgttgtg
     5941 ctcgccggtg aaacggagac aaccggaagc ggatttaccg tcacggttac ctgcccggta
     6001 gatgagcatc cgttaccatc tgttcccgtc actgtatacg ttgttgtcga agccggtgta
     6061 accgtaaccg gggaaccaga taaacttccg ggcatccacg tgtacgatgt cgcacccgaa
     6121 ccggtgagcg aagtggaaga gccggcgcag atggtcggtg aagctgcggt tgctgtaaca
     6181 gtcggcgtcg gattgacggt aaccgttacc tgcgcattgg aagaacatcc attggtggtt
     6241 cctgtaactg tgtacgtggt gttggaagtc ggagagactg taacggaaga tcccgacagg
     6301 tttcccggca tccaggtgta ggatgtagcg ccggaagcat tgagcgtcgt agagctcccg
     6361 ttacagatcg tgggatttgt agcagaaacc gtaagcgtgg gcggaggtgt caccgttacg
     6421 gtaactgaag ctgtgttcga gcagccggga ccgttggaac cggtgaccgt atacgttgtc
     6481 gtcgcacccg gcgtaaccgt aacggaagaa ccggagagat ttcccggcat ccaggtgtaa
     6541 gtagttgcac cggatgcagt cagcgtggtg gaaccgccag tgcagatccc gggacttgat
     6601 gcagaagccg tgacagtcgg aggcggaccg acagttacgg taaccggtat tctccagaaa
     6661 cccgggcagg tggtaacgta atatgtcgtt gtagtgccga gcgcgggcgt tgtgtatgtg
     6721 cttcctgttc ccagctgcgt tcctgcagtt gccgcatcgt accagataat ggttgtgcca
     6781 cccggaacgg ttccggaaag cgtagccgta agcgtcgtgc ttccgccggc gcagatcgtc
     6841 acgttcgttg cggatatcgt gaaattggtg atcgatccgt tgctggtacc cgggcatcct
     6901 ttcgtcgcgc cgttcggcaa aaattcggta agaccgagag agtttgttgt tccgttatta
     6961 ccgccattcg cattccttac cggtgctccg gaagaaacag cgatgtaacc gccaccgccg
     7021 ccgccgcccg gaccttcagc ttccgtggta agcgaaacgt tctggttgcc gccgttcccg
     7081 ccattggcat ttaccgtcag cgtatttgcg actgtgctta ctgcattcag gacgatcgtg
     7141 ccgcccgctc ctcctccacc ggctgcatca atcccgtttg ccgtacctcc gtcatttcca
     7201 ttggcgacga ttgttcccgt accgttaata tttccgaatg acatgaggta gatcaaaccg
     7261 cctccgtcac ctccgttacc tcccgcgccg ttgttctggt caccggcacc gccaccaccg
     7321 cccataaaaa gacggccacc ggaataatca agcggtcgcc cgccaagccc ggttgcctgc
     7381 gggttccgtg aatcgccacc ccagagtgca ttgccgggag ccgttgttgt cgcattcctg
     7441 ttattggaag aaaaagaata tcctcctctt ccgccgcccg ccgaattagc ggctgtccgc
     7501 agcgacatcg tattgacagg cggttcgagg ttccaggctg tagtccagcc cgctcccgac
     7561 tgatccggga ttccgttacc gctccagagc gcaccgggat tggaaccatt cggtgcattg
     7621 gcaccacctc cgcctccggc attgtggcca tcgccaccgc ccccggcatt tgccgctgcg
     7681 ccacgacagt aacttccgcc aaacgcaccg ccgtatgtat tgtattgccg catataaccg
     7741 gcaacccctt ctcccctggc cgcaccgagc gtgctgtcgc tgttgaagac cacggcgctg
     7801 ccgtaggacg tggcattatc gctgttaagg gcaccgccgc ggaaaccttt cccggtggca
     7861 tcgatcgttc cgtttaccgt aatatcacca gaggtttcaa ccgcaagaat tccaccgatc
     7921 gttccgttcc agtcatcgca ggaaagcgta gcgcccgcgt tgatcgtgag attgatataa
     7981 cggggaacgc ggatcacttc agggttgcag gcttcgccac caagatctcc attgtggtaa
     8041 tatgtgtacg ttgttccgca atcgatgttg atggtggtaa gattgggaac cgaaaccacc
     8101 tggtgaagct catagcgtcc cgcactgtta taatcccagg gattcactat ggcgccccag
     8161 gaagtatccg actggtttgc aacggaccag ccatcacgga gccagacgcc ctgcggctgg
     8221 taaatcatga ccaggtcacc cgggcgaaga ttccatccga agcggccgtt cgcattcatg
     8281 ttgctgttgg cgacagtgat cgaagtgctt ccggcgggca cgtacccggt gatggcggtg
     8341 tactcattga cgatcgtcgt cccgcttgaa agcgtgagtg atccgttgat accctgctga
     8401 gcgcgggcaa ctgaaagaga cagggtaaat aaaatggtaa tgaatacgtt gaaacgtttc
     8461 atgcagaaaa atcttaggac aggtgaagtt gagcggatac taatttagga gaataagacg
     8521 ggaatcccaa atgtcaccgc acgctgaaaa tcagtcagtc ccaaccgttt ccgccttgaa
     8581 cggattacgg ggaatccatt cggatggctt gaggtattcg atgaacggac ggatgagctg
     8641 gttggaaaga ctgttgcggt gacggatata gcagacgagg tcgtgggcca ctgcgccgac
     8701 agttgatttt atttccgaag ccgttcttcc gagataaacc gaaggaaccg gagatgtgat
     8761 cccttcattc acaaaatcgt aaaggatgcg ctggtaaagc gggttccttt cattcgcgtc
     8821 gtaatccagg ccgatgaaat gcgcttcgag atgcgattta agaaggaaag aagacctgaa
     8881 cgcaaccggt tcgccaccta ccaggtatac gtgcaggcgg aatttgccgg ggaatgcgtt
     8941 cttcatcgcc gaaaaataat tctttcccaa aggcgccaac cggaatttcg cccttgcatg
     9001 aacgttcatg tacagcgaat agatcttttc ttcatgggca gcgatctttt caattcccca
     9061 ttcctcacaa acgacagtct ccgtatcctt catcactttt ttcgcgcggt tgcggtattt
     9121 cttgctcatc gattccaggt aatctccgaa agttttccat tcgcttcgta tgtccaccac
     9181 catctcgggt tcgaccatga accggtggta tctttttttc ttcagggaag aagacggatg
     9241 ctctttcttt ttttcgaaga aatccttgac caggatcgcc gagatttttg cacgtgaacg
     9301 gtcgtggccg gtgatgtatt ttacgacggg cgggatagcc ctgtagatct tctccgcatc
     9361 gtattccttt ttcaccgcga cgcaatattc cccgctgatg aaattattcc cgcagatgag
     9421 aatgcgcatt cctttttctt cgcgcaactt tcccacaatg ccggagaatg tcgttcccga
     9481 accgagaaac ccggcagcgg aaaccgccgg cttcagaatt ttgacgatct catcagcggc
     9541 gagtgttatc acctggaagc acaccacgcc tacggcctgc tgcttttccc ggatgaccgc
     9601 gtaccagaac gaaacgttcg gatctgctgc ctgctcaaac gcggagagaa attccgccga
     9661 aagaaaaagc gaagaacccg ctgccacgga attccactcg cgggaagaaa acccggaaac
     9721 ggaattgaaa aattctgcgc tcagcccgcg gggcatgtcg tcagtaatca catgtttggc
     9781 acgggtcttt cccactttcg gttcaggtgc aaggactgta agaaacagtt ccgggataat
     9841 atgtttggcg acaagacagt taagcatccc ctttaagtcg cccccgcaag gggaaatatt
     9901 ttaccaaaga taaaatttca tcgtgcgagg atgtcgatca tgttcagcaa ctcggggttg
     9961 ggttcctgca tctgcttgac agccagctcc agcggcgtaa aagtgatctt tccgctcacg
    10021 attccggtca tcaactgtct tttccctgcg atgagcgctt tcaccgcctc atagccgatg
    10081 cggctggcat tgacgcgctc catgcaggac ggcgatccgc cccgctgtat atgcccgaga
    10141 atagtcacgc gcacatcaag cgaaggaaaa cgtttcttca cttcttcgct tacacgaaaa
    10201 gcgccgccgg cttcatcgcc ttcggccacg atgatgatct ttgaacgttt gtcttcccgc
    10261 ccggaaccga gccgctccat cacgttccgc atgtcgttcc tgctttcagg aatgagaatt
    10321 ccttcggcgc ccgcaccgat gccgctgtac agcgcgataa gtcctgcatc acgtcccatc
    10381 acttccacga tgaaaacacg gttgtgcgat tcggccgtgt cgcggatctt gtcgaccgca
    10441 tcgatgaccg tgttgatggc ggtatcgaaa ccgatcgtgt aatccgttcc gttgagatca
    10501 ttgtcgatcg ttccgggaca accgatcacg gggacggagc atatttttga aaattccact
    10561 gcgccgcgga atgttccgtc tccgccgata gcgatcattc cttcaatgcc gtgcttttcc
    10621 acctgcgctt tcgcctgcgc tcttccttcc ggcgccagga aacgtttgct gcgcgcagtt
    10681 ttgagaatgg ttcccccgcg ctggatgata ttcgcaacgg aagatgcatc cattccgaaa
    10741 aaatccccgt cgatcatgcc ttcataaccg taacggatac cgagaacttc aagtccttcc
    10801 gccaccgccg tgcgcaccac cgcgcgaagg caggcattca ttcccggcgc gtcgccaccg
    10861 gaggtaaaga ccgctatttt tttcattcgt ggtcaggctt taactgagtt atttcttctt
    10921 tcgcggccgg taatcgttca atcctccggt ccgtcctctt tccttagctt atgaatcttt
    10981 tttgtacctg cgtacatttc aaatttgagc agtttgcatt cgagcggccc gttgaacaat
    11041 gttatttttc gtgaaggcct cagcccgatg acttttatcg cctccatgtt cgaggtaata
    11101 agccatgctt catagccgga atattttttc ttgaacgtgt cgccgatctg cttgtagaaa
    11161 acatctgaat catctttgtc catgcgctca ccgtaaggcg gattcatgat gagcacgcct
    11221 ttcccttcgg gcggcgtcca ttcgaaaaaa tcggctgtga atgtcttcac cacgtcctcc
    11281 acttttgcgc ggagaatatt ttcccgcgct ttacgcagca cgttctgcga aatatcgaag
    11341 gccgttatat cctgctgctc gctgctgatg cggtcgagcg cggcgtccgt gattttttcc
    11401 cacagctctt tatcgaagtc attccatttt tcgaagccga acttttcgcg gaaatagccg
    11461 ggcggaatgt gattggcgta cagcgccgct tcgatcgcga tggtgcccga accgcacatc
    11521 gggtcgatga gacggaaacg cgggttccat tctgtgagct gcacgatgcc ggctgcgagc
    11581 acttcgttca tgggcgcacg gcccgtgtcc tcccggtaac cgcgcttgtg aagcgaatag
    11641 cccgagctgt cgagtgaaat attcacctcc tcccgcgaca cgtgcacgtt gatgcgcagc
    11701 gccgggaatt ccgtatcgac cgaagggcgc cgtccgaatt tatcacggaa ctggtcgacg
    11761 atcgcatctt ttgttttctg cgcgatgaaa tacgagtggt tgaaaatttc cgtattgagc
    11821 gtgcagtcga ccgcgagcgt atcgtcgggc gtcatccact gcgtccagtc gatcgcatag
    11881 acgccgtcat aaaaatcctt ttcgttcttc gcatggaacg acgaaacggg cttcagtatc
    11941 cgcagcgccg tgcgcaggag gtagttcgcc ttgtacatcg tgcctttgtc gccaatgaaa
    12001 ctgaccgcgc gcttatgctc ttcgatctcc cgcgcaccga tacgcagcaa ttccttcgca
    12061 aggatatttt cgagtccctg catggttttc gccaccatcc ggaattcgcc gggcttcaaa
    12121 actggttgtt gattttccac gtcccgaaga tagcgcctat ttggccgggc cgtgaccatt
    12181 aattaatttt cctcaatggg aaaccggatc tcaaacaaac gcgtaaacga ccaaagcatc
    12241 aaaatgagca acggtctgac ggctgtaatt atggacgtca tctgccttgc aggttccggt
    12301 ctcgctgaaa cggaacatga aaaagatctc atgatctggt tcgctcagaa ggaccaggat
    12361 attttcggga tcgggatagt tggttttgac gtttcggata tgggatggaa aaaagaaatt
    12421 ttcgaagcac agaaatcatt tatcctggaa gtcattgaca gcgccgcgca gaaaacaggc
    12481 tgggagaagc tgaattattc tcctaaagaa gaccgggtgc ttcccgtgct gaaggaatta
    12541 cgtgaaatga tcatggcctt ttccgaaacg catgcggaga atgccgtccc gctgaagatt
    12601 taccccttcg aagaaaagat catgaaatac gacaggtgcg aaaaacaccg cgtttacctt
    12661 cataccctgg gctgtatcgt ctgcaataac gccgcgctcg gttaacccgc catcactccg
    12721 tcacccgatc accgcccacg ccacggcaac accgaacagg atcgtcacga acttgatgag
    12781 gttgaaccgg tgattttccg tgctctcgaa caggatcgtc gtggaaatgt ggaggaaaat
    12841 accgacgact attcccgtga tcgccgaaaa gtaaggtgag agatcaccct gcaggttctc
    12901 tccgatgaca tagctcccga tcattcctgc cggcgtcatc agcgcgaaaa gcgaaagaaa
    12961 aaggaacgat ttcatcttcg aattcccgtt gtgcagaagc agtcccatga atgcgatggc
    13021 gatgggaatg ttgtgcagca cgattcctgt caggaaaggc gggaatattt tcggatcgga
    13081 gctgg
//
LOCUS       DASTSF010000265         2475 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1576567,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000265 DASTSF010000000
VERSION     DASTSF010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2475
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1576567"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..403
                     /locus_tag="VFU15_07670"
     CDS             <1..403
                     /locus_tag="VFU15_07670"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEU4717695.1"
                     /translation="NAWTNNAQYFYTYNANNSRLSDLHQSWQNSNWVNSDSTHYYYHT
                     VTGIAEENTAMRISAFPDPSGGIFTLSLPGNTGAYDLFITDVTGKTIFSSAGNTGSSR
                     AIDLSGQPAGIYFCTVSFRNGPVSHAKIMIR"
     gene            407..865
                     /locus_tag="VFU15_07675"
     CDS             407..865
                     /locus_tag="VFU15_07675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717696.1"
                     /translation="MWYLFSEWLVLAIFVALPVLLVIGRFTLRKKKLFDSARLVSFVL
                     FLGSISVSLYFLFFQPYRLDDVTDRCGRVISNPNMINNPEILLFWCAACYSWINLLTT
                     LTGKTAHVILCLLVPVILLIVSFVAMQRTSVENLSPEKRESIYDGMGGRG"
     gene            883..1329
                     /locus_tag="VFU15_07680"
     CDS             883..1329
                     /locus_tag="VFU15_07680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717697.1"
                     /translation="MKKHIFSLLFLVTGILISLAWLLLYVASANLDVHDTYFVLSPYV
                     PVGGALLLYLFFAGIYFALTVKKRAILKIPAYIHYLLTTLLFLTLTVSPLLFTGGSPP
                     NITRGTAGILYVLNLLTGISALLFICGQFLFLFIVIYALRRKDPSS"
     gene            1352..1804
                     /locus_tag="VFU15_07685"
     CDS             1352..1804
                     /locus_tag="VFU15_07685"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEU4717698.1"
                     /translation="MKKILLSLFAGLLCAAANAQFVARMEVKEPLPGACDSTNVYALF
                     TGFTGQIPPVAPWDEKQLGAKLQNIQFLKDNPKFKGKMMINCIINCKGEMIKCTVDNK
                     SGNDELDKQVLAIFQDMKKWTPGTLDGNAVDTTVLYSIEIKKGKITVS"
     gene            2069..>2475
                     /locus_tag="VFU15_07690"
     CDS             2069..>2475
                     /locus_tag="VFU15_07690"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU4717699.1"
                     /translation="MAAKKTRIAKSKTVFTALQVYFYVRSMDRSVDFYTKTLGFPLRV
                     RFGNFWAEVDAGPVTIGLHPGKKGKAKPEEGGILSLGVKDIENVYTSLKEKKVKVGKM
                     HTPDRGKFFMITDPDGYELHVVEFDPKWKKANR"
BASE COUNT          635 a          668 c          586 g          586 t
ORIGIN      
        1 caatgcgtgg accaacaacg cgcaatattt ttacacgtat aacgcgaaca actcccggct
       61 cagcgatctt caccagtcgt ggcagaattc caattgggtg aacagcgatt ccacgcatta
      121 ttattaccac acggtcaccg gcatcgcgga agaaaacacg gcgatgcgca tctctgcgtt
      181 cccggatcct tccggcggaa ttttcaccct cagcctgccg gggaataccg gtgcgtatga
      241 tctcttcatc actgatgtca caggaaaaac aattttcagt tccgccggca acacaggttc
      301 ttcccgcgcg atcgatctgt ccgggcaacc ggcaggaatt tatttctgca ccgtcagctt
      361 caggaacggg cccgtttccc acgcgaaaat catgatccgc tgatcgatgt ggtacctgtt
      421 ttccgaatgg ctggtactgg cgatcttcgt tgccctgccg gtcctgctgg tcattggtcg
      481 tttcacgctc cggaaaaaaa agttattcga ttccgcacgt cttgtgagct tcgttctttt
      541 tctcgggagc atttccgttt ccctttattt tctttttttt caaccgtaca ggctcgacga
      601 tgtaaccgat cgctgcggtc gcgtcattag caatcccaac atgataaata acccggagat
      661 cttgctcttc tggtgcgcag cctgctattc ctggatcaac cttctcacca cccttaccgg
      721 caagacggcg catgttattt tgtgcctgct tgtgcccgtt atcctgctga tcgtttcttt
      781 cgtggcgatg cagaggacgt cagtggaaaa cctttcgccg gaaaagcggg aaagcattta
      841 cgatggcatg ggaggaagag gataaactac tcatccatgc caatgaaaaa acatattttc
      901 tccctgctgt tcctcgttac aggaattctt atttcgctgg cctggctcct gctgtacgtc
      961 gcttccgcca acctggacgt gcatgacacg tatttcgtgc tgagtccgta tgtaccggtc
     1021 ggcggtgcgt tgctgcttta cctgtttttc gccgggatct atttcgcgct gacggtgaaa
     1081 aaacgggcta tcctgaaaat accggcctat attcattacc tgctgaccac gctgcttttt
     1141 cttacgctca cggtctctcc tcttcttttc accggcggca gtccgccgaa catcacccgc
     1201 gggacagccg gaattcttta cgtgctgaac ctgctgaccg gcatttccgc gctgctgttt
     1261 atctgcggac aattcctatt tttattcatc gtgatctacg ctttgcggcg aaaagatccc
     1321 tcttcctgaa tcaaccctta aaaccccgaa catgaaaaag atcctcctct cactttttgc
     1381 cggcctgctt tgtgcggcgg cgaatgcgca gtttgttgcc cgcatggaag tcaaggaacc
     1441 gctgcccggc gcctgcgatt cgaccaatgt ttatgcttta tttaccggtt tcaccgggca
     1501 gatacccccg gtagctccct gggacgaaaa acagctcggg gcgaagctgc agaatatcca
     1561 gttcctgaaa gacaacccga aattcaaagg caaaatgatg atcaactgca tcatcaattg
     1621 caaaggcgaa atgatcaaat gcacggtcga caataaatcc ggcaatgacg aactcgacaa
     1681 gcaggtgctg gccattttcc aggacatgaa aaaatggacg cccggcacgc tggacgggaa
     1741 cgcggtcgac acaaccgtgc tgtacagcat cgagatcaaa aagggcaaga tcactgtttc
     1801 ctgattaaat ccaggataca gaaacggcaa actgtttttt caaacacaga gaacacgaag
     1861 ttttgcacaa agctcacaaa gaagatccgg acaggatgaa cccggaacta caaaccgaaa
     1921 acattcaggt tccacatccc aaacggcaaa ctgttttttc aaacacagag aacacgaagt
     1981 cctgcacaaa gctcacaaag aaaatccgga caggatgaac ccggaactga aaactgaaaa
     2041 ccgttcaacc tgatcacaaa aaacacacat ggccgcaaaa aaaacacgca ttgcaaaaag
     2101 caaaaccgtt ttcaccgcgc tgcaggtgta tttctatgtg cgcagcatgg atcgctccgt
     2161 cgatttctac acgaaaacgc tgggattccc gcttcgggtc cgtttcggaa atttctgggc
     2221 ggaggtagat gccggcccgg tgacgatcgg gctgcatccc ggtaaaaaag ggaaagcgaa
     2281 gccggaagaa ggcggcatac tttcgctcgg cgtgaaagac atcgagaacg tgtacacttc
     2341 cctgaaagag aaaaaagtga aggtgggaaa gatgcacacg cccgaccgcg gaaaattttt
     2401 catgatcacc gatcccgacg ggtacgagct gcatgtggtg gaattcgatc cgaaatggaa
     2461 gaaagcgaac aggta
//
LOCUS       DASTSF010000266         5731 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_818012,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000266 DASTSF010000000
VERSION     DASTSF010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5731
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_818012"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="VFU15_07695"
     CDS             <1..220
                     /locus_tag="VFU15_07695"
                     /inference="COORDINATES: protein motif:HMM:NF014165.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HEU4717700.1"
                     /translation="AQFFAHAKKAIPSLEKEIEAGNFSSLLKWLRENIHRHGKRFEAK
                     ELCTRVTGEPLKFSYFMDYAKEKYGKLY"
     gene            complement(389..1723)
                     /locus_tag="VFU15_07700"
     CDS             complement(389..1723)
                     /locus_tag="VFU15_07700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717701.1"
                     /translation="MTRRLRFILLFFFFAGTLFSQESRLLVMGRVISAKDSVSGIDGV
                     VITCNAPQRQTHSNKDGYYSLLLDYKTGQVITYRIIGSKTEYRTITKKMLDRAGGDTL
                     RLDVYLSPDLITLPLAEVKSGPDTVLGNNKFFIEDYMFYDEHCFILLTFEKSLKSAKV
                     MLTDDHQAILSSFDVPGEAIELYKDYQGNINVMCRDSAFRVKVVQPAEVLLLALPYKD
                     FCERMLPCVDTVGSRILFTNYMRDYPAFSYFSYNPADTTVEPVHYIVDKELLAQYNWE
                     FDYLLPKDRLYARKMEMYTGIDKRIIAATMTGFPQSIYYTPLYAPLFVVHDTVCVFDH
                     YSDTLFLFDKFNRSIGASHIDYHHPKNWKEWDRQMLQDDVTGDIYAQFEKGGFYYLKR
                     IDIKTGKVTGTYKLQNQYVKHIRVHDGEVYYIYRPFESVQTKFLYREKMELS"
     gene            1946..3277
                     /locus_tag="VFU15_07705"
     CDS             1946..3277
                     /locus_tag="VFU15_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010601715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEU4717702.1"
                     /translation="MKKQVLGYFDVSMIVVSLVIGMGIFKTPGNVAREALTPDVFYLA
                     WIFGGITALCGALTFAEIGSRLPVSGGYYRIFSHSYHPAFAFMLNGTILLSNAASVAG
                     VALIGAEYISALVFHGTYSPAETETHRLVISAIEIILFFGLNMLGLKTSATAQNILTV
                     FKLVVVAVLCCSVFVPAATTVALPPAGNLSSLDYLKSFGLCLIPISFTYGGYQQTINF
                     GGEVENAPRTVPRAIITGMAIVIVLYLLINFVYVQVIGFDQLKSADSIASLLAERLFG
                     ASGGIALRFLLFFSVLAYVNVGLMSNPRVIMAMSEEKTLPALFSRHSRKFNVPVPALI
                     AFTVATIAILFFAKTFDQLVKYVIFLDSLGFIGAAFTIFILRRKKTGEEKTIYRLKLY
                     PFVPALFILAYGIITAAVISDNWRYAFIGLCIFFAFLPLYFIFTVTRKKEQ"
     gene            3279..3740
                     /locus_tag="VFU15_07710"
     CDS             3279..3740
                     /locus_tag="VFU15_07710"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEU4717703.1"
                     /translation="MNVDCYRLLGIGPGADEKEIRKAWRLRSKDVHPDVNASADAAKK
                     FRELTDAMNLLLDPAERIKHDRQFGYFSKPKNQDANAKESFSEFQEEKAEHLVNAWNE
                     DYEKAMQMREQQRREVIEKHRGRLKRILISTLLFIVFLSALLIAMWYKNML"
     gene            complement(3800..4546)
                     /locus_tag="VFU15_07715"
     CDS             complement(3800..4546)
                     /locus_tag="VFU15_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002759945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HEU4717704.1"
                     /translation="MTVTPFSSVSSSAPGLPGKVVPAAPGLKGFDTVATISASLAAQF
                     VKSGYSFCARYLSLGQGQNAGDLTAAEATDILNGGLALIAVQRVRKEGWVPSASLGSS
                     TGTNAANNGKSVGLPAGMNVFLDLEGVASGTSAQAVIDYCQAWYAAVSAAGYVPGIYV
                     GAGSVLNSSQLYSNLSFQHYWKSQSKVPDVAVRGYQLVQAYPSVQVNGIYIDEDTTQN
                     DNKGGTVLWLAKQPTLAQNPANISGAGKNA"
     gene            4777..>5731
                     /locus_tag="VFU15_07720"
     CDS             4777..>5731
                     /locus_tag="VFU15_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014164312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU4717705.1"
                     /translation="MNYDVIVIGSGPGGYVTAIRASQLGLKTAIVEKAELGGICLNWG
                     CIPTKALLKSAQVFEYLKHASDYGITVNGGSPDFGAVVKRSRDVANGMSKGVQFLMKK
                     NKIDVIAGYAKVKPGKKVEVQGADNSISTLEAKHIIIATGARSRELPNLKQDGKKIIG
                     YREAMNLPSQPKSMIVVGSGAIGVEFAYFYAALGTKVTVVEFLPNIVPVEDEEVSKQL
                     ERSFKKQGIEVMTNASVEKVDASGAGCKVTIKAKDGEKVMDCDIVLSAVGISSNIENI
                     GLEEVGIATDKGKILVGKYYETNIPGYYAIGDVIGTQALAHV"
BASE COUNT         1349 a         1499 c         1550 g         1333 t
ORIGIN      
        1 ggcgcagttc ttcgcgcatg cgaaaaaagc gatcccttcc ctcgaaaaag agatcgaagc
       61 gggaaatttt tcttcgttgc tgaaatggct gcgtgagaat attcaccgtc acggaaaacg
      121 gttcgaagcg aaagagcttt gcacgcgcgt gacgggcgag ccgctgaagt tttcctactt
      181 catggattac gcgaaagaga aatacggaaa attatattga cctggcgggc agattgcaat
      241 tccgttgccg gtcgcgaagg ttgtttcgtt cttgttaaaa aaggaacttc tgcacaaact
      301 gcctgaagta caggtttcgg gtgacgaccg aaaaacggaa aaacggaaac ttttttcgcg
      361 gtgtcgaatg ggaagttcgc aggaaaaatc agctcagctc cattttttcc ctgtacagga
      421 attttgtctg caccgattcg aacgggcggt agatgtaata cacttccccg tcgtgaacgc
      481 ggatgtgctt cacgtactgg ttctgtaatt tgtacgtgcc cgtaactttt cctgttttga
      541 tgtcgatgcg cttgaggtaa taaaaaccgc ctttttcaaa ttgcgcataa atatctcccg
      601 tgacatcatc ctgcagcatc tgccggtccc actctttcca gttcttcggg tgatgataat
      661 cgatgtgcga agcgcctatg gaccggttga atttatcgaa caggaaaagc gtatcggaat
      721 aatgatcgaa cacgcagacg gtatcgtgca cgacgaagag cggcgcgtac agcggcgtgt
      781 aatagatcga ctgcggaaat cccgtcatcg tcgccgcgat gatgcgcttg tcgatgcccg
      841 tgtacatttc cattttgcgc gcatacagcc tgtccttcgg caggagataa tcgaattccc
      901 agttgtactg ggcgagcagc tctttatcga cgatgtaatg gaccggttcc accgtcgtgt
      961 cagcgggatt gtacgagaaa tacgagaacg ccgggtaatc gcgcatgtaa ttcgtgaaaa
     1021 gtatccggct tcccaccgta tcgacgcagg gcagcatgcg ttcgcagaaa tctttataag
     1081 gcagtgcaag gagcagcacc tcggcgggtt gcacgacttt gacccggaag gcgctgtccc
     1141 tgcacatgac attgatgttt ccctggtaat ctttgtaaag ctcaatggct tcaccgggaa
     1201 catcgaatga gctcagtatg gcctggtgat cgtcggtgag catcactttc gccgacttca
     1261 gcgatttttc gaacgtgagc aggatgaaac agtgctcatc gtaaaacatg taatcttcga
     1321 tgaagaattt attgtttccc agcaccgtgt ccggaccgct tttcacttct gcaagcggga
     1381 gcgtgataag atcaggtgac aggtagacat caagacgcag cgtatctccg ccggcacggt
     1441 cgagcatttt cttcgtgatg gtgcggtatt ccgttttgct tcctatgatg cggtaggtga
     1501 tcacctgccc tgttttataa tcaagcagca gggaataata gccgtctttg ttcgaatgcg
     1561 tctggcgctg cggtgcattg cacgtgatca cgacaccgtc gatacccgaa acagaatctt
     1621 tcgcagagat cacgcggccc atgacgagca ggcggctttc ctgggaaaaa agcgttccgg
     1681 caaaaaagaa gaacagcagg atgaaacgta agcggcgggt cataagcttc tttaaagata
     1741 gacgcgatgg tcctgtttct ccatacgttt tacgcgaagc aaatacagtt tgtaaaagaa
     1801 ttccggtaag tggatggttc cggcaaacag gtggcaaccc ggttccgggt ttcaaggtag
     1861 agcgtctcaa atctcctatc ttacgtctca cgcctaatgc ggtatatttg accttcgcgc
     1921 agaacaaata aacccggcag cgaaaatgaa aaaacaggtc ctcggctatt tcgacgtaag
     1981 catgatcgtg gtgagccttg tcatcggcat gggcattttc aaaacacccg gaaacgtggc
     2041 acgcgaagcg ctgacgccgg acgtgtttta cctcgcgtgg atcttcggcg gaataaccgc
     2101 gctgtgcggc gcgctcacgt ttgccgagat cggttcgcgc ctgccggtga gcggcggcta
     2161 ttaccgcata ttttcccatt cgtatcaccc tgcctttgcg ttcatgctca acggaacgat
     2221 cttgctttcc aacgcggctt ccgtggcggg cgtggcgctt atcggggcgg aatatatttc
     2281 cgcgcttgtt tttcacggaa cgtattcccc ggcggaaaca gaaacgcacc gcctcgtcat
     2341 ttcagcgatc gagatcattc tctttttcgg gctcaacatg ctcggcctga agacaagcgc
     2401 cacagcgcag aatatcctta ccgttttcaa gctggttgtt gtcgccgttc tctgctgctc
     2461 ggtttttgtt ccggccgcaa cgaccgtcgc gctgcctccc gcaggaaacc tttcgtcgct
     2521 cgattacctg aaatcgttcg ggctttgcct gatcccgatc tctttcacct acggcggcta
     2581 ccagcagacg atcaacttcg gcggagaagt ggagaacgct ccgcgcacgg ttccgcgtgc
     2641 gatcatcacg ggcatggcaa ttgtcattgt gctgtatctc ctgatcaatt tcgtgtacgt
     2701 gcaggtgatc ggtttcgacc agctcaaatc ggccgactcg atcgcgtcgc tgctggcgga
     2761 acggctcttc ggtgcttcgg gcgggatcgc gctgcgcttc ctgcttttct tttccgtgct
     2821 ggcgtatgtg aatgtggggc tgatgtcgaa cccgcgcgtg atcatggcga tgagcgaaga
     2881 aaaaacgctg ccggccctgt tcagccgcca cagcagaaaa ttcaatgtgc cggtgcctgc
     2941 gctgatcgcg tttaccgtgg ccaccattgc gatccttttc tttgccaaaa cattcgacca
     3001 gctggtgaaa tacgtcatct tcctcgattc gctcggcttc atcggcgccg cgttcacgat
     3061 tttcatcctg cgcagaaaaa aaaccggcga agagaaaacc atctaccggc tgaaattata
     3121 cccgttcgtt cccgcgctct tcattctcgc ctacggcatc atcactgcag ccgtgatctc
     3181 cgacaactgg cggtacgcct tcatcggcct ctgcattttc ttcgctttcc ttccgttgta
     3241 ttttattttt acggtgaccc ggaaaaagga acagtaacat gaacgtcgat tgctaccggc
     3301 tgctgggtat cggtcccggc gcagatgaga aagagatccg caaagcgtgg cgcctgcgtt
     3361 cgaaagacgt tcatcccgat gtgaacgcat cggctgacgc ggcgaaaaaa ttccgcgagc
     3421 tgaccgatgc gatgaacctt ctcctcgatc cggccgaacg cataaagcac gaccggcagt
     3481 tcggctattt ttcaaaaccg aaaaaccagg atgcgaatgc gaaggaaagc ttttctgaat
     3541 tccaggaaga aaaagcggag catcttgtca acgcctggaa tgaagattat gaaaaagcca
     3601 tgcagatgcg cgaacaacaa cgccgtgaag tgatcgaaaa gcaccgcgga agactgaaac
     3661 gcattttgat ctccacgctg ctgttcatcg ttttcctgtc ggcgctgctg atcgcgatgt
     3721 ggtacaaaaa tatgctgtga aaattcccgc tgctgccgca ttttgcagga acagccggac
     3781 gtaaacgcgg aaaaattcct caggcattct tccccgcgcc ggaaatgttt gccgggttct
     3841 gcgccagcgt gggttgcttg gccagccaca gcaccgttcc acctttgttg tcgttctgcg
     3901 tcgtgtcttc gtcaatgtaa attccgttca cctggacaga cgggtacgcc tgcacaagct
     3961 ggtagccgcg cacggcgaca tcaggaactt tgctctgcga tttccagtaa tgctggaagg
     4021 aaagattgga ataaagctgg ctggaattca gcacggatcc cgcaccgaca tatatgccgg
     4081 gaacatatcc cgccgccgaa acagcagcat accacgcctg gcaataatcg atgacggcct
     4141 gtgcgctggt gccggaagcc acgccctcga ggtcgagaaa aacattcatg ccggccggca
     4201 gcccgacgga tttcccgttg ttcgccgcgt ttgttcccgt gctgctgccc agcgaagcgc
     4261 tcggcaccca gccttctttc cgcacacgct gcacagcgat cagtgcaagt ccgccgttga
     4321 gaatgtccgt tgcttccgcc gcggtgagat cgccggcgtt ctgtccctgg ccgagcgaga
     4381 gataacgcgc acagaacgag tagccgcttt tcacgaattg cgcagcaagc gatgcgctga
     4441 tggtggcgac ggtgtcgaaa cctttgaggc cgggtgcggc cggaacgact ttgccgggaa
     4501 gtcccggtgc ggaagaagaa acggatgaga acggggtaac ggtcatggtt tgttttttta
     4561 tgaaatgtaa caaaaatgta tttgaagcga cacgaaaaat ttcaaaccaa tcaagttccg
     4621 gggaacaaaa cggaccgccg ggcagtgcgg ttatttctcc gctgcgcggc ttttgctgtc
     4681 aaacatcacg taagcaacgt ttaccgtctc ctgaccggcg tctagtacct agcgtctatt
     4741 ttcctatttt cgcagtccaa tccaatttca ttttttatga attacgacgt catcgttatc
     4801 ggaagcggcc ccggcggata cgtaaccgct atccgtgctt cgcagctcgg actgaaaacc
     4861 gccatcgtgg aaaaagccga gctcggcggg atctgcctca actggggctg cattcctacg
     4921 aaagcgctgc tgaaaagcgc gcaggtattc gaatacctga aacacgcttc ggattacggc
     4981 atcacggtga acggcgggtc tcccgatttc ggcgctgtgg tgaaacgcag ccgcgatgtg
     5041 gccaatggca tgagcaaagg cgtgcagttc ctgatgaaga agaacaagat cgacgtgatt
     5101 gccggctatg cgaaagtgaa gcccggtaaa aaagtggaag tgcagggcgc cgacaattca
     5161 atttcaacgc ttgaagccaa acacatcatc attgccacgg gcgcacgttc acgcgagctg
     5221 cccaacctga agcaggacgg caaaaagatc atcggctacc gcgaagcgat gaaccttccc
     5281 tcccagccca aatcgatgat cgtcgtgggt tccggtgcga tcggcgtgga gtttgcgtat
     5341 ttctatgcgg cgctcggcac gaaagtgacg gtggtggaat tcctcccgaa cattgttccg
     5401 gtggaagacg aagaagtttc gaaacagctc gagcgttcgt tcaaaaaaca aggtatcgag
     5461 gtgatgacga atgcaagcgt ggaaaaagtg gacgcttccg gcgcaggctg caaagtgacg
     5521 atcaaagcaa aggacgggga gaaagtgatg gactgcgaca tcgtgctttc ggccgtgggc
     5581 atttcttcga acatcgagaa catcgggctc gaagaagtgg gcattgcaac ggataaagga
     5641 aagatcctcg tcggaaaata ttacgaaaca aatatcccgg gctactacgc gatcggcgac
     5701 gtgatcggca cacaggcgct ggcgcacgtg g
//
LOCUS       DASTSF010000267         4693 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_36810,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000267 DASTSF010000000
VERSION     DASTSF010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4693
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36810"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..962
                     /gene="hemE"
                     /locus_tag="VFU15_07725"
     CDS             <1..962
                     /gene="hemE"
                     /locus_tag="VFU15_07725"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009187031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HEU4717706.1"
                     /translation="MRQAGRVLPEYREVRAKMGGFIELVKTPEFAAEVTVQPVDILGV
                     DAAIIFSDILVIPEAMGLPYKMVEARGPWFENTVQSQSDIEKLRVANGEEDLSYVCDA
                     IRLAKAALAGSVPLLGFAGAPWTILAYMVEGSGSKTFSKAKKFLYTQPRAAHMLLEKI
                     AESTIHYLKAQVKAGADMVQLFDSWAGILSPEQYREFSLRYISLICDEIHPLVPITVF
                     AKDAHFIRRALGQLNCDVVSLDWTMDIKESRAMIGENKTLQGNADPCLLYADIETIKS
                     ETEKMLRAFGPHRHIANLGHGLYPDLEKEKVKFFVDTVKNFRF"
     gene            1225..3618
                     /locus_tag="VFU15_07730"
     CDS             1225..3618
                     /locus_tag="VFU15_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019986873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU4717707.1"
                     /translation="MKNIFSFFILVLACACLPAQSTQTIRGTVVDLESKSPLPGAVVS
                     VMKDSVTIGGAAADINGKYTVSNIPVGRYTVVVRFLGYKPVSLPNVLVISGKETILDF
                     EMEQSTVTNQEVVIKATNKQGTVNDMGSGSGRMFSVDETNRYAGSRGDPARMASNFAG
                     VQGADDSRNDIVVRGNSPMGVLWRLEGVDIPNPNHFAIEGTTGGPVSILNNKTLSNSD
                     FFTGAFPAEYGNSVAGVFDLRMRNGNNMKHEFTGQFGFLGTELAAEGPISKKSGSSYL
                     VTYRYSTLQLFESLNIPIGTGAVPNYQDAAFKLNFPMKKGGTVSMFGIGGMSKIDIIV
                     SKYTQPTDELYGMDSRDQYFRTGMGVAGIVYMKPVNENTFFRVVVAGSHRYSAAHHNL
                     VWRDSSFAVDSITPKMGYRNTEDKYSVNFYVNRKFGSHHTLKAGMMNDLFVDFLTDSV
                     HSEVMNRFYNRQDYTGTSALIQPYVQWKYKPNDKVTVNLGVHAQYFTLNGSSSVEPRA
                     GMKYDFTEKQSLNVGVGLHSQMLPAYVYYTHLTSDTAAPYYLQNKDVGFMHSAHAILG
                     YDLMISENMRIKVETYYQYLYGVPVEKRSSSFSVLNQGSGFSRFFPDTLVNEGTGYNA
                     GVELTVEKFFSKKYFFMFTASLFDSKYRGSDGILRNTDFNTHYAVNVLGSREFHLNDR
                     QTLELGAKLTLAGKRYYTPVDTVASRLDNDVYYVDSLRNTLQFSKAYFRLDARVVWRY
                     NAKNITHEIGVDFINVTNNKNILSLTYSPDQKDPTKYDVVEQYQLGFLPLFYYKIDF"
     gene            3622..4212
                     /locus_tag="VFU15_07735"
     CDS             3622..4212
                     /locus_tag="VFU15_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019947821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEU4717708.1"
                     /translation="MRELKSLLVYCGASAGEDPVYRRVASELGKAMAEKKIRLVYGGG
                     SIGLMGVVADAVMQNGGEVHGVIPGFLDVKEVRHKGITQMHVVSSMHERKAMMEKLCD
                     AAIALPGGFGTMDEFFEMLTWAQLGLHSKPLGLLNVKGFYDHFLAQLDVMVKEKFLTP
                     SNRALVIGAVSVGELLGKISSANPVAEKKWMNEGQE"
     gene            complement(4254..>4693)
                     /locus_tag="VFU15_07740"
     CDS             complement(4254..>4693)
                     /locus_tag="VFU15_07740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717709.1"
                     /translation="EEENLHVRFINMNNIYCCFLLLGSRDDIRRATEVMEGLLFNYQQ
                     VAFHRIYDQIFGTLIMGYFMLEDYVKVQECYKRYEKLTANSSKFPENDITIKGFYYVS
                     QWIVSGRKQYQEKLNSLLTKTEGVRNLDSTKEMLLDLKEYYNC"
BASE COUNT         1111 a         1205 c         1302 g         1075 t
ORIGIN      
        1 tcatgcgcca ggccgggcgc gtgctgccgg aataccgcga ggtgcgcgcg aagatgggcg
       61 gctttatcga gctcgtgaaa acaccggaat tcgctgcgga agtcaccgtg cagcccgtcg
      121 atattctcgg cgtggacgcg gcgatcattt tttcggatat cctcgtcatt cccgaagcga
      181 tgggtcttcc ctacaaaatg gtggaagcgc gcggaccctg gtttgaaaat accgttcagt
      241 cgcaaagcga tatcgaaaag ctgcgcgtgg cgaacgggga agaggatctt tcctacgtgt
      301 gcgatgccat ccgcctggcg aaagctgcgc tggccggaag cgtcccgttg ctcggtttcg
      361 ccggtgcgcc gtggacgatt ctcgcctaca tggtggaagg aagcggctcg aaaacatttt
      421 cgaaagcgaa gaaattcctg tacacacagc ccagggcggc gcacatgctt ctcgaaaaga
      481 tcgcggaatc caccattcat tacctcaaag cacaggtgaa agcgggcgcc gacatggtgc
      541 agctgttcga ctcgtgggcg ggaattcttt cgccggagca gtacagggaa ttttcgctcc
      601 gttacatttc gctgatctgc gatgaaattc acccgctcgt tcccataacc gttttcgcca
      661 aggacgcgca ttttatccgc cgcgcgctcg ggcagctgaa ttgtgatgtc gtgagcctcg
      721 actggacgat ggacatcaaa gaatcgcgcg cgatgatcgg tgaaaacaaa acgctgcagg
      781 gaaatgcgga tccgtgcctt ttgtacgcag atattgaaac gataaagagc gaaaccgaaa
      841 aaatgctgcg tgcattcgga cctcaccgcc acatcgccaa tctcggccac ggcctttatc
      901 ctgatctcga aaaggagaaa gtgaaattct tcgtcgatac cgttaagaat ttcaggttct
      961 gatcgcagga aatttgaaga gaaggtgaat tttccttagc cccgggcgca gtggttatgc
     1021 ggcagcattt gaacggagca cgggaagaag cgtacgtgaa atgcgcaaca gatgcgcttc
     1081 aaaacaggcc ggtttgcaaa aaatgatttt cagggcatac gttttctgca aatgttgctc
     1141 cctcattaat ttcttttact cattttgcca atacggttga cacgcttccg gttctgatta
     1201 tttttggcca gaaatcaccc gtttatgaaa aacattttct cctttttcat tcttgttctc
     1261 gcctgtgcgt gcctgcctgc gcaatccacg cagacgatcc gcggtacggt tgtcgatctc
     1321 gaatcgaaat cgccgctgcc cggcgccgtt gtttcggtga tgaaggattc ggtgacgatc
     1381 ggcggcgcgg ccgcagatat caacgggaaa tacacggtga gcaacattcc cgtgggaaga
     1441 tataccgtcg tggtgcgttt cctcggctac aaaccggttt cgcttccgaa tgtgctggtt
     1501 atttccggca aggaaacgat cctcgatttc gagatggagc aatcgacggt gaccaaccag
     1561 gaagtggtga tcaaagcgac gaacaagcag ggaacggtga atgacatggg atcgggaagc
     1621 ggaagaatgt tttcagtgga tgaaacgaac cgttacgcgg gaagccgcgg cgacccggcg
     1681 cgcatggctt cgaattttgc gggcgtgcag ggagcggacg attcacgcaa cgatattgtc
     1741 gtgcgcggaa attccccgat gggcgtgctg tggcgactcg aaggcgtgga cattcccaac
     1801 ccgaatcatt tcgcgatcga aggaaccacg ggcggtcccg tgagcatcct caataataaa
     1861 acgctctcca attccgattt tttcaccggc gcgttcccgg cagaatacgg caacagcgtg
     1921 gccggtgttt tcgacctgcg catgcgcaac gggaataaca tgaagcatga gttcaccgga
     1981 cagttcggtt ttctcggaac ggagctggcc gccgaaggac cgatcagcaa aaaaagcggt
     2041 tcgtcttacc tggtcacgta ccgttattcc acgctgcagc tgttcgagtc gctgaacatt
     2101 cccatcggca cgggcgctgt tccgaattac caggacgctg cgttcaagct gaatttcccg
     2161 atgaagaagg gcggaacggt ttccatgttc ggcatcggtg gcatgagtaa gatcgacatc
     2221 atcgtcagca aatacacgca gcctacggat gaactgtacg ggatggacag ccgcgaccag
     2281 tatttccgca cgggaatggg cgtcgccggc attgtttaca tgaaaccggt caatgagaat
     2341 acgtttttcc gcgtcgtcgt tgcgggaagt caccgctatt ctgccgcgca tcacaacctc
     2401 gtctggcgcg attcatcgtt cgcggtagat tccatcacgc cgaaaatggg ttacaggaac
     2461 acggaagaca aatactcggt caatttttat gtcaaccgga aattcggaag ccaccacacg
     2521 ctgaaagcag ggatgatgaa tgatcttttc gtggatttcc tcaccgacag cgtgcacagc
     2581 gaagtgatga accgttttta caaccgccag gattatacgg gaacgtcggc gctcattcag
     2641 ccttacgtgc agtggaaata caagcccaat gataaggtga cggtgaacct gggcgtgcat
     2701 gcgcagtatt tcacgctgaa cggaagcagt tcggtggaac cgcgcgcggg aatgaaatat
     2761 gattttaccg aaaagcaatc gctcaacgtc ggcgtcggtc tgcacagcca gatgcttccc
     2821 gcgtacgtgt attataccca cctgacatcg gatacggctg cgccttacta tctccagaac
     2881 aaagacgtcg gcttcatgca cagcgcgcac gcgatcctcg gctacgatct catgatcagc
     2941 gagaacatgc gcatcaaagt ggaaacctat taccagtatc tctacggtgt gccggtcgaa
     3001 aagcgatcgt catcgttctc cgtgctcaac cagggttccg gcttcagccg ctttttcccc
     3061 gatacgctcg tgaacgaagg cacgggctac aacgccggcg tcgagctgac cgtggaaaaa
     3121 ttcttcagca aaaaatattt cttcatgttc accgcttcgc tcttcgattc gaaatacagg
     3181 ggaagtgacg gcatcctgcg caacaccgat ttcaatacgc attacgccgt gaacgtgctc
     3241 ggctcgcgcg agtttcatct taatgacagg cagacgctgg agctcggggc gaagcttacg
     3301 ctcgccggga aacgctatta cacgccggtg gacacggttg cgtcgcgcct cgataacgat
     3361 gtgtattacg tcgattcgct caggaacacg ctgcagttca gcaaagcgta tttccgcctc
     3421 gatgcgcgcg tggtgtggcg ttacaacgcg aagaacatca cgcacgagat cggcgtcgat
     3481 ttcatcaacg tgacgaacaa caagaacatt ctcagcctca cgtattcgcc cgaccagaaa
     3541 gacccgacga aatacgacgt ggtggagcag taccagctcg gattcctgcc tttgttttat
     3601 tacaagatcg atttctgaaa aatgcgtgag ctaaaatcgt tgctggtcta ttgcggcgct
     3661 tccgccggtg aagatcctgt gtaccgccgt gtggcgtcgg aacttggaaa agcgatggcg
     3721 gaaaaaaaga tccggcttgt ttacggtggc ggcagcatcg ggctgatggg cgtggtggcc
     3781 gatgccgtga tgcagaacgg gggagaagtg cacggcgtta tccccgggtt tctcgacgtg
     3841 aaggaagtgc ggcacaaagg cattacgcag atgcacgtcg tatcctcgat gcatgagcgc
     3901 aaggcgatga tggaaaaatt atgcgacgcg gcgattgcgc tgccgggcgg gttcggaacg
     3961 atggatgaat ttttcgaaat gcttacctgg gcgcagctcg gcctgcacag caagccgctc
     4021 ggtctgctga atgtgaaagg tttttacgat cattttctcg cgcagctcga cgtgatggtg
     4081 aaggagaaat ttctcacacc ttccaatcgc gcgcttgtga tcggcgctgt ttccgtcggg
     4141 gaattgctgg gaaaaatttc ttcggcgaat cccgttgcag aaaaaaaatg gatgaacgaa
     4201 ggacaggaat agaacggggc gccggctgtc ggtcagcgat cgtcaatcag ccgtcagcaa
     4261 ttgtaatact ctttcaggtc aagcagcatt tccttcgttg aatcaagatt gcgcacgcct
     4321 tctgttttgg tgagcagcga gttcagtttt tcctggtact gttttcgtcc tgaaacgatc
     4381 cactgcgaaa cataatagaa tcccttgatc gtaatgtcgt tctcgggaaa cttcgagctg
     4441 ttggctgtga gcttttcgta acgtttgtag cattcctgca ccttcacgta atcttcgagc
     4501 atgaaataac ccatgatgag cgttccgaag atctggtcgt agatgcggtg aaaagccacc
     4561 tgctggtaat tgaagagaag tccttccatg acttccgtcg cgcgccgtat gtcatccctg
     4621 ctcccgagca gcaggaagca gcagtagatg ttgttcatgt tgatgaaacg cacgtgcagg
     4681 ttctcttctt ccc
//
LOCUS       DASTSF010000268         3370 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1358554,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000268 DASTSF010000000
VERSION     DASTSF010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3370
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1358554"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..925
                     /locus_tag="VFU15_07745"
     CDS             <1..925
                     /locus_tag="VFU15_07745"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="potassium channel protein"
                     /protein_id="HEU4717710.1"
                     /translation="IDPQFTFLDALYMTVNTIGTVGFREVHPLSAGGQVFTIFLIITG
                     FGTFAFALTAFTSYIINGEWHQYYRITRQMTTIEKLHGHVIICGYGRNGRQAAHNLKK
                     HNRRFVVIENKKEVVDSLTHRYSDLVINGDATQDEVLENAGVKRAAALITTLPIDADN
                     LFIVLSARALNPKLTIISRASDDNSDRKLKTAGANNVIMPDRIGGAHMASLVIKPDVI
                     EFIDHITGQGGPDINLEEITFDLLPEHLRNKTIRDLEIRNKSGANIVGFKTGNGDFII
                     NPDPETKFTPAGKLFVLGTPEQIGKLKEMLS"
     gene            1100..2035
                     /locus_tag="VFU15_07750"
     CDS             1100..2035
                     /locus_tag="VFU15_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015809217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU4717711.1"
                     /translation="MKTILITGTNGLLGQKLVYALINRNRKHADWKIIATSRGANRLN
                     RKDGYTYVPLDITQRNEVETVFAEYRPDVVINTAAMTNVDACESKKEECVALNISAVE
                     YQLRALEKMKSGKYDPQFIHLSTDFVFDGENGPYKEEDQPDPLSYYAWSKLESEVLTK
                     NSSLRWAIVRTIIVYGIVDNMSRSNIVLWAKGALEKGQKINVVDDQFRSPTLAEDLAE
                     GCILCAEKNAQGIFHISGKDTMSVLELVYRVADFWKLDRSLITPVKSETIAQPAKRPP
                     RTGFIIDKAKKVLGYNPHSFEEGLSVLDEQLIKNS"
     gene            complement(2053..>3370)
                     /locus_tag="VFU15_07755"
     CDS             complement(2053..>3370)
                     /locus_tag="VFU15_07755"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEU4717712.1"
                     /translation="EESLRLRKEIGETLGYFATLLRYTDLHLEEKKFDDARRIAQEGL
                     SIATKEKVNFGIIRFLQMLGKVEMTTGNLAAAKKNYEEALAFAEQFSFKSIVYELHLA
                     IAQVDKLGGDYESAFDHFEKSVKIKEEVVNYQSNTRLKSIQMFTRIESAQRETELERK
                     KNEELKAAYDIIEERNKDITDSIRYAKRIQDGFLPNDEELEELLGEHFVLFLPRDIVS
                     GDFYWATGVTTTRSNDANARMKVVAAVDCTGHGVPGAFMSILGNMLINQTIRNPDVNS
                     PAEALDYLNRELPKNLKAQNREETIRDGMDLSMIAIDPANMKMHFAAANNPVYFVRDG
                     ILTELKGDKQAISGSEDIPKKPFTGRSVSLKKGDCVYLLTDGFADQFGGPRGKKFKYR
                     QLQEQLVSLHDRPMKEQKQLLETIFREWKGDLGQVDDVLIIGIRVN"
BASE COUNT          786 a          950 c          880 g          754 t
ORIGIN      
        1 catcgatccg cagttcacgt ttctcgatgc gctgtacatg acggtcaaca ccatcggcac
       61 tgtcggtttc cgtgaagtgc acccgctctc ggccggcggg caggtattca ccatttttct
      121 catcattacc ggttttggta cttttgcatt cgcgctgacc gccttcacga gctacatcat
      181 caatggcgaa tggcaccagt attaccgcat cacgcgacaa atgacaacca tcgaaaaact
      241 ccacgggcat gtgatcatct gcggttacgg acgtaacgga aggcaggccg cgcacaacct
      301 caaaaagcac aaccgccgct tcgtcgtgat cgaaaataaa aaagaggtgg tggattcgct
      361 cacgcacagg tactccgacc tcgttattaa tggcgacgcc acgcaggatg aagtcctgga
      421 gaacgccggc gtgaaacgcg cagcggcgct catcaccacc ctgcccatcg acgccgacaa
      481 tcttttcatc gtgctttcag cgcgtgcgct caacccaaag ctcaccatca tcagccgcgc
      541 gtccgatgac aattccgacc ggaagctgaa aacagccggc gccaacaacg tcatcatgcc
      601 cgacaggatc ggcggcgcgc acatggcttc gctcgtgatc aagcccgacg tcatcgagtt
      661 catcgatcac atcaccgggc agggcggacc cgacatcaat ctcgaagaga tcacgttcga
      721 tctccttccc gagcatctcc gcaacaaaac catccgcgat ctcgagatcc ggaataaatc
      781 gggcgcgaac atcgtcgggt tcaaaaccgg caacggcgat ttcatcatca accccgatcc
      841 cgaaacgaaa ttcacacccg cgggaaagct gttcgtgctc ggtacgcccg agcagatcgg
      901 gaagctgaag gagatgctga gctgaaaaac gccggagatt caattcgacg gcggacggca
      961 atgaggagga gcgcaacaac gtgccgttta tcagctgctt tcctgctgtc gctattgcac
     1021 ggcaaaacgc tccatcaggg ctatgcgatc agcacagctg ctcattaaaa ccacagaacc
     1081 aacaaccggc aatcggaaaa tgaaaaccat cctcatcacc ggcaccaacg gcctcctcgg
     1141 gcagaaatta gtatacgcgc ttattaatcg caacaggaag cacgcggact ggaagattat
     1201 cgccacgtcg cgcggtgcga accggctgaa tagaaaagac ggctatacct acgttccgct
     1261 cgacatcacg cagaggaacg aggtggaaac tgttttcgcg gaatatcggc ccgacgtcgt
     1321 gatcaacacc gcggcgatga cgaacgtcga tgcgtgcgaa tcgaaaaaag aggaatgcgt
     1381 ggcgctcaat atttcagcgg tggaatacca gctgcgcgcg ctggaaaaaa tgaagtcggg
     1441 gaagtacgat ccgcagttca tccatctctc caccgatttc gtcttcgacg gggagaacgg
     1501 cccttacaaa gaagaagacc agcccgaccc gctcagctat tacgcgtggt cgaagctcga
     1561 atcggaagtg ctgacaaaaa attcttccct gcgctgggcg atcgtccgca ccatcatcgt
     1621 gtacggcatc gtcgacaaca tgagccgctc gaacatcgtc ctctgggcga aaggtgcgct
     1681 cgaaaaaggg cagaaaataa atgtcgtcga cgaccagttc cgttcgccca cgctggcgga
     1741 agacctggcg gaaggctgca tcttatgcgc ggaaaaaaat gcgcagggaa tttttcatat
     1801 ttccgggaaa gacacgatga gcgtgctcga gctggtttac cgcgtggcgg atttctggaa
     1861 gctcgacagg tcgctgatca cgcccgtaaa atcggaaacc atcgcgcagc ctgcgaaacg
     1921 cccgccgcgt accggcttca ttatcgacaa agcgaaaaaa gtgctcggct acaacccgca
     1981 ttcattcgaa gaaggacttt ccgtccttga tgaacagcta ataaaaaaca gttaagaata
     2041 aagccccgat cattaattaa ccctgatccc gataatgagc acgtcgtcca cctgcccgag
     2101 atcgcctttc cattcgcgga agatggtttc cagcaactgc ttctgttctt tcatcggccg
     2161 gtcgtgaaga gaaaccagtt gttcctgcag ctggcggtat ttgaattttt ttccgcgggg
     2221 gccgccgaac tggtccgcga aaccgtccgt gagtaaataa acgcagtcgc ctttctttaa
     2281 gcttacgctc cggccggtga atggtttttt aggaatgtcc tcgcttccgc tgatcgcctg
     2341 cttgtcgcct ttcagctccg tgaggatccc gtcccgcacg aaataaacgg ggttgttcgc
     2401 tgctgcaaaa tgcattttca tattggccgg atcgatcgcg atcatggaca ggtccatgcc
     2461 gtcgcggatc gtttcctcgc ggttctgtgc tttcaggttt ttcggcagct cgcggttgag
     2521 gtaatcgagc gcttcggccg gagagttgac atccgggttg cgtatcgtct ggttgataag
     2581 catgttcccg aggatgctca tgaatgcgcc gggaacgccg tgtcctgtgc agtcgacagc
     2641 agccaccact ttcatccgtg cgttcgcgtc gttgctgcgc gtggtggtaa ccccggtcgc
     2701 ccagtaaaaa tctccgctga cgatatcgcg cggcaggaac aaaacgaaat gctcgccgag
     2761 cagttcttcc agctcttcgt cattcgggag aaaaccgtcc tggatgcgtt ttgcataacg
     2821 gatgctgtcg gtaatatcct tgttgcgttc ttcgatgatg tcgtacgcgg ctttcagctc
     2881 ttcattcttc ttgcgttcga gctccgtttc gcgttgtgcg gattcgatcc gggtgaacat
     2941 ctggatactt ttgagacgcg tgttcgactg gtagttgaca acttcctcct tgatctttac
     3001 ggatttttcg aaatgatcga aagcggattc gtaatcaccg ccgagcttgt cgacctgtgc
     3061 gatggcgagg tgaagctcat acacgatgga tttgaacgag aactgctctg cgaacgcaag
     3121 cgcttcttcg taattctttt ttgcggccgc aagattcccg gttgtcattt ccaccttgcc
     3181 gagcatctgc aggaagcgga tgatgccgaa gttcactttt tccttggtgg cgatcgacag
     3241 tccttcctgc gcaatgcgcc gcgcatcgtc gaattttttt tcttcaaggt gcaggtcggt
     3301 gtaccgcagc agcgtcgcaa aatagccgag cgtttcgccg atctcttttc gcaggcggag
     3361 actttcttcg
//
LOCUS       DASTSF010000269        11489 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_688288,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000269 DASTSF010000000
VERSION     DASTSF010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 11489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11489
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_688288"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="VFU15_07760"
     CDS             <1..250
                     /locus_tag="VFU15_07760"
                     /inference="COORDINATES: protein motif:HMM:NF019262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="c-type cytochrome domain-containing protein"
                     /protein_id="HEU4717713.1"
                     /translation="NCNTAGCHSGTSPAGGLNLEPANAYAQLTSHQGGYIDTLHPEFS
                     LLYSQLISTTTPMPPTGKLDDCTIKLVLKWIQQKAKNN"
     gene            295..2361
                     /locus_tag="VFU15_07765"
     CDS             295..2361
                     /locus_tag="VFU15_07765"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEU4717714.1"
                     /translation="MKFFSIKTLTITAIFLLSVAHLEAHWTSKGPYGGSVTCFAVADT
                     NVFLGTAEGGVFRSVNQQFLSWKYYNYTGLTSPKITAITAIGTKVIAGTMDSGAYVSS
                     NMGVSWAMHNSGLSNLYITSLITSGPAVFAGTMGGGVFMSADSGTTWMAMNTGLTNMM
                     PVTFALNGTDIYAGTMGGGIFVSTNNGVSWSAVNTGLTDMNIHGLTVSGNSIFAATGS
                     GVFASPTSSISWTLSNTGLGNTYVNGLNSSGGYVFAATNGGVYATPDASVSWVSANTG
                     LVNDTIQAVGIYGTTMFAATKNSGAYKASVLTMSWSKMNTGLNNLETYAIYNSNQLVI
                     GATNKGVYVSRDLAASWTASNSGLTDSLFVTGFTFAGSVLFASTQHGGVFMSNDTGTT
                     WMPMNAGLTTMDITQIIATNTDLVAAAANGNIFHTPISTINWVLCTGVTPGMTFTSIA
                     TDGNTTFIGTNGNGVYMAMTSSSSSWMADNSGLGNMNVTSLAVHGSNVFAGTNGGGVF
                     KSDMMMSPWTAVNSGLPTMNIESLCAAGQYVVAGYRGGVHATFDDGNSWLPPNVELYI
                     PAYADVKAISFTSASTRVFLSTPANSYYSNNITELPSGIGDVQPQADAFSVYPNPNNG
                     TFRVDLADENSHIMLISIYDGLGNLLQQIDHPAQNVIEMGYPAGIYFIEVRTDRGGSV
                     QKMIIR"
     gene            2495..2935
                     /locus_tag="VFU15_07770"
     CDS             2495..2935
                     /locus_tag="VFU15_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017731030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEU4717715.1"
                     /translation="MSSTKETYCSRVKQSWHSIARMYNTEGIRHDLTTTVGFILLQIN
                     TKEGIPSTKIGPAIGMEATSLVRTLNQMEQKGLIVRERDKKDARKVMIKLTEKGKQKR
                     DVSRRVVKGFNEEIERRIGSSKLKVFNEVIEKINAIAGERKLTD"
     gene            2946..5351
                     /locus_tag="VFU15_07775"
     CDS             2946..5351
                     /locus_tag="VFU15_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014681381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEU4717716.1"
                     /translation="MKRIIRKVAVLGSGVMGSRIACHFANIGLEVLLLDIVPRELTPA
                     ETTKGKTLEDKTVRNRIVNDALQSVLKSNPSPIYRKAFVSRITTGNFDDNLAEISSCD
                     WVIEAVVERLDIKQQVFEKVEKFRRKGTLITTNTSGIPIHLLTQGRSDDFNENFCGTH
                     FFNPPRYLRLLEIIPGPHTKQDVLSFLLHYGELYLGKTTVLCKDTPAFIANRIGVFGI
                     MALFHLVEKMGLTVEEVDKLTGPVLGRPKSATFRTCDVVGLDTLIHVANGLDQNCPDD
                     EAHPLFTLPAYVSKLAENKWYGDKTGQGFYKKVKNASGKSEILALDLKTMQYNPSQKV
                     KFATLEQTKPIENLRERMSVLIAGKDKAGEFYRASFFALFAYVSNRIPEITDDLYKID
                     QAMNAGFGWELGPFEAWDALGVAETVKAMEAAGNKPAQWIYDMLSSGASSFYRFDNGT
                     KLCYDIPSKKYKTIPGTEDFILLEPLRGTKTVWKNSGATVTDIGDGILNLEFHTKMNT
                     IGGEVLQGLNTAIDMAEKDFRGLVISNEGGNFSAGANLGMVFMFAVEQEWDELDFAVR
                     AFQAATGRVRYSSIPVVVAPHQLTLGGGTEICLHADAVVAHAETYMGLVEFGVGVIPG
                     GGGTKEFATRLADELHEGDVELNSFRNRFLTIGQAKVSTSAYEAFDLGYLRTHKDRVV
                     MSRNRLLAEAKMEAVRLAEEGYTKPVMRHDIRVLGRQALGLAYVGANSMQSGKYISEH
                     DSLISRKLAWVMCGGDLSQPALVSEQYLLDLEREAFLSLCGERKTLERMQSILTGGKV
                     LRN"
     gene            5465..6640
                     /locus_tag="VFU15_07780"
     CDS             5465..6640
                     /locus_tag="VFU15_07780"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002999742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEU4717717.1"
                     /translation="MNAYIIAGLRSAVGKAPRGLFRFLRPDDLATQVIRQVVAQVPNL
                     DKEQIDDVIVGNAMPEAEQGLNVGRLISLLALDTDKVPGMTVNRYCSSGLETIAIAAS
                     KIHAGLADCIIAGGVESMSLIPMGGWRVIPNTDIAANHPDWYWGMGLTAEAVANEYNV
                     SREDQDAFALSSHLKAISAIKEGKFRSGIAPVTVKEIFLDEKNKRQSREYIVDTDEGP
                     RADTNAEALAKLKPVFAEGGSVTAGNSSQTSDGAAFVIIVSEKMLKALNVQPVARLVS
                     YAAAGVPPRIMGIGPVHAIPKALAKAGMKLGDIDLIELNEAFASQSIAVQRELKINPD
                     ILNVNGGAIALGHPLGCTGAKLTVQVLNELKARKKKYGMVTMCVGTGQGAAGIFEMM"
     gene            6872..8251
                     /gene="creD"
                     /locus_tag="VFU15_07785"
     CDS             6872..8251
                     /gene="creD"
                     /locus_tag="VFU15_07785"
                     /inference="COORDINATES: protein motif:HMM:NF008712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HEU4717718.1"
                     /translation="MTPPQQPPVPQVPFQLPQKPKKENPSITVKLLVIALLVLILVVP
                     WGLLQWLIYDRQNMNEEAVNEVSQKWSNPQNVSGPVLYVPFIATSSGGSDYTAYLKVL
                     PEELSISGKVVPEKRYRGIYEVVVYSSALSVTGKMKIPDYEEMKIDAKKIMWDRAFVG
                     IGVDDMRGIRDGVNFKWRDASYSCNPGISTGTDGYSSSTGVSCPVAFAHTDTVPGAEI
                     PFSFDLDLDGSKNLNFEPLGKTTKVHLTSTWASPSFSGAFLPDTHNINTNGFVADWKV
                     LHLNRNYPQAWVDNAYNTADSRFGVELMVPVDHYTKSSRASKYAIMLIGLTFLIYFFV
                     EVMQKLRIHPFQYILVGLSICVFYLLLLSFSEHMVFNFAYWTAASLTLLAITSYSGAI
                     FRKKLPTAVMGTSLLLMYGFMFVIIQLEDYALLVGSVALFLILIVLMYLSRKIKWYGD
                     DENNPTSTT"
     gene            8264..10042
                     /locus_tag="VFU15_07790"
     CDS             8264..10042
                     /locus_tag="VFU15_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003012118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEU4717719.1"
                     /translation="MSNAIKGGEFLIRETNAADIFIPEEWDEESKMIAQSCNDFLQQE
                     VFPNLDRIDQQEEGLMVSLLDKAAALGLLGISVPEEYGGFGKDFKTSMLTTEMLGAGH
                     SFAVAIAAHTGIGTLPILYYGNDAQKKKYIPKLANGEWKACYCLTEPGAGSDANSGKT
                     KAVLSPDGKHYVINGQKMWITNSGFAEIFIVFAKIDDDENLSAFIVEKSFGGVTLNPE
                     EHKMGIKGSSTRQVFFNDCKVPVENLLSERGNGFRIAVNILNVGRMKLAGAALGASKR
                     VCSVATQYANERQQFGRPIAKYGAIRHKLAEMAIRIYALESTLYRATQSIDDAIKALQ
                     AGGMEFEKAKLKGTEQFAAEAAILKVNGSEVLNYAVDEGVQIYGGMGYSAEAPMERAY
                     RDARINRIFEGTNEINRMLIVDMLLKRAMKGELDLMGPAQKVAGELMAIPDFGAPDTT
                     LFAAEKKTLQQFKKAILLVAGAAVQKLMMQLAKEQEVLMNISDMLIDLYTAESLQLRV
                     EKLVSRNGEENCAVQLDILRTFIYDAADRIHKAGKDAINSFAEGDEQRMMLMGLRRFT
                     KVEVFNSKESRRRIAAKMLEENKYCW"
     gene            complement(10080..>11489)
                     /locus_tag="VFU15_07795"
     CDS             complement(10080..>11489)
                     /locus_tag="VFU15_07795"
                     /inference="COORDINATES: protein motif:HMM:NF026253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HEU4717720.1"
                     /translation="EIADLGTDSLRRIVVYDRFIVCVKDERFGCVDYSGRTVVPFLYD
                     ALAYDEQYSEFIFWENGLTGLMDSTGKKLTEARFDFIRPFCGNFAEVNDNSNNKRNTN
                     NLLSREGDLLFPPGQYDSLYMIWHNYPGWDPGRDGYLVVNPRHGHGVVSGSGKLIVPD
                     TFLAVCASGDGYAAYTRFYWFLFDSAGRQVGNERVKPPVIGWREMERPVYKIGNVFAD
                     SKGQVLITGDYDGTLDEYDYIYPFYYDNYIRYRRPSSGDSEVFMIRHKDKLIDVYTMS
                     GKKLDGISGRAAYLTRFDRRMDNFIFGDAGAYYGKDQRLYGLMDSTGRVILPKVYRRI
                     STDVTDSTRLIAIDSTGSMLYFDFHGNPVDPPLYSQVYYPELKSGLLLRAVNGKYGLT
                     DKSGKVIVPFVYDELVELDPGYFCYKRDGKFGLLRADGTVITPPRYSAPLQATTHSRL
                     FFIYNEGFIDIYGREYWKN"
BASE COUNT         2858 a         3135 c         3084 g         2412 t
ORIGIN      
        1 gaattgcaac acagccggat gccattcggg aacatccccg gcaggaggac tgaaccttga
       61 accggcgaat gcgtatgcgc agctcaccag ccaccagggc ggatatatcg atacgctcca
      121 cccggaattc agcctgctgt acagccagct gatctccacc accacgccga tgcctcctac
      181 gggaaagctc gacgactgca cgatcaaact cgtgctcaaa tggatccagc agaaagcgaa
      241 aaataattga cgcggaaacc ggatccctgt cattcactca aaaaacaaac caacatgaaa
      301 ttcttttcta tcaaaacact taccataact gcaattttcc tcctgtccgt cgcgcacctt
      361 gaagcgcact ggacaagcaa aggcccgtac ggcggctccg tgacctgctt cgctgtggcg
      421 gacaccaacg ttttcctcgg aacagcggaa ggcggcgttt tccgcagcgt caaccagcag
      481 ttcctgtcgt ggaaatatta caactacacg ggactcacga gtccgaaaat aacagctatc
      541 accgcgatcg gaacaaaggt gatcgccgga acgatggaca gcggcgcata cgtttcttcc
      601 aacatgggtg tcagctgggc gatgcacaac agcgggctca gcaaccttta cattacttcc
      661 cttatcacga gcggccctgc ggtttttgcg ggaacgatgg gtggcggtgt tttcatgagc
      721 gcagattccg gtacgacatg gatggcgatg aacacaggtc tcacgaacat gatgcctgtg
      781 acgttcgcgc tgaacggtac agatatttat gccggtacaa tgggcggcgg aatcttcgtg
      841 agcacgaaca acggcgtatc atggagcgca gttaatacgg gactgacgga tatgaacatt
      901 cacggtctta ccgtgtccgg aaactcgatc ttcgctgcaa cgggcagcgg cgttttcgcc
      961 agcccgactt cctccatcag ctggacactt tccaatacgg gactcggcaa tacatatgtg
     1021 aacggcctga attcttcagg aggttatgtc ttcgctgcaa ccaacggcgg cgtttatgcg
     1081 acgcccgatg catcggtttc ctgggtttcc gccaatacag gtctcgtgaa cgacacgatc
     1141 caggccgtgg ggatttacgg aacgacgatg ttcgcagcga cgaagaacag cggcgcttat
     1201 aaagcaagcg tactcacgat gtcatggagc aaaatgaaca ccgggctgaa caatcttgaa
     1261 acgtacgcga tctacaattc aaaccagctg gtgatcggcg cgacgaataa aggcgtttac
     1321 gtcagccgcg accttgccgc atcatggaca gcttccaaca gcggtcttac cgacagtctt
     1381 ttcgtcaccg gtttcacttt tgccggaagc gtgcttttcg caagcacaca acatggcggt
     1441 gtgttcatga gcaatgacac gggtacaacc tggatgccga tgaatgcagg gctcaccact
     1501 atggatatta cgcagatcat cgcaacgaac accgacctcg tggctgcagc ggcaaacggg
     1561 aacatttttc atacgccgat cagcacgatc aattgggtgc tttgtacagg agttacgccc
     1621 ggtatgactt ttacttccat tgcgacagac ggcaatacca cgttcatcgg tacaaatgga
     1681 aacggcgtct acatggccat gacaagctcc agcagcagct ggatggcgga taacagcggt
     1741 ctcgggaaca tgaatgtaac ttcgcttgct gtgcacggtt ccaatgtttt tgccggtaca
     1801 aatggcggcg gagtctttaa atccgatatg atgatgtccc cctggacggc agtcaacagc
     1861 gggttgccaa ccatgaacat tgaatcgctc tgcgcggccg ggcaatatgt tgtcgccggc
     1921 taccggggag gcgtgcatgc caccttcgac gacggtaact catggctgcc gccgaacgtg
     1981 gagctctaca ttcccgccta tgccgacgtc aaggccatct ctttcacttc ggccagcaca
     2041 cgcgtgttcc tttccacgcc ggcaaattcg tattactcca acaacatcac ggagcttccg
     2101 agcggtatcg gggatgtaca gccacaggct gacgcattct ccgtttaccc gaacccgaac
     2161 aacggaacgt tccgggtcga tcttgccgat gagaattcgc acattatgtt gatcagcatt
     2221 tacgacgggc ttggcaacct ccttcaacag atcgatcatc ccgcgcagaa tgtcattgaa
     2281 atgggatatc ctgccggcat ttatttcatt gaagtgcgca cagaccgcgg cggttcggtg
     2341 cagaaaatga tcatccggta atatttattt ttttagtgaa agaaaaagtc cgggctcccg
     2401 gactttttct tttttccatt gaaagcgcga agaagaaacg tgattttcgt cattttatat
     2461 gcatgcatag gataattttc ctaatttgca tcccatgtct tcaaccaaag aaacctattg
     2521 ttcccgtgtc aagcaatcct ggcattccat tgcaaggatg tacaacaccg agggcatccg
     2581 ccacgatctc acgacgaccg tcggcttcat tcttttacag atcaacacca aagaaggaat
     2641 tccttccacg aagatcggtc ccgccatcgg catggaagcg acgagcctcg tgcgcacgct
     2701 gaaccagatg gagcagaaag ggctgatcgt ccgtgagcgg gataaaaaag atgcgcgcaa
     2761 agtgatgatc aagctgacgg aaaaagggaa gcagaaacgg gatgtctcgc gcagagtggt
     2821 gaaaggcttt aacgaggaga tcgaacgccg catcggttct tcgaagctca aagtgttcaa
     2881 cgaagtcatt gaaaaaataa atgccattgc cggtgaacgc aaactcaccg attaatcgtt
     2941 cagtcatgaa aagaatcatc cgtaaagttg ccgtcctcgg ctccggcgtc atgggatcgc
     3001 ggatcgcgtg ccatttcgcg aacatcggcc tcgaagtgct gctgctcgac atcgttccgc
     3061 gcgagcttac accggcggaa accacgaaag gaaaaacgct tgaagacaaa accgtgcgca
     3121 accgcatcgt caacgacgcg ctgcagtcgg tgctgaaaag caacccctcg cctatttacc
     3181 gcaaagcgtt cgtgtcgcgc atcacgaccg ggaatttcga cgacaacctc gcggagatct
     3241 cttcctgcga ctgggtgatc gaagcggtgg tggaacgtct cgacatcaag cagcaggtgt
     3301 ttgaaaaagt ggagaaattc cgcaggaaag gaacgctcat cacgacgaac acatcgggca
     3361 ttcccattca cctgcttacg cagggccgtt cagacgattt caacgaaaat ttctgcggaa
     3421 cgcatttctt caatcccccg cgttacctgc gtttgctcga gatcattccc ggtccgcaca
     3481 cgaaacagga cgtactcagt ttcctgctgc attacggcga gctttacctc ggtaaaacga
     3541 ccgtgctctg caaggacacg cccgctttca tcgcaaaccg tatcggcgtg ttcggcatca
     3601 tggcgctttt tcaccttgtc gaaaaaatgg gcctgacggt ggaagaagtg gacaaactga
     3661 cgggtcccgt gctcggccgc ccgaaatcag ccacgttccg cacgtgcgat gtcgtcggtc
     3721 tcgacacact tatccatgtg gcgaacggtc tcgaccagaa ttgtcccgac gacgaagcgc
     3781 atccgctgtt cacgctcccg gcttacgttt cgaagctcgc ggaaaacaaa tggtacggtg
     3841 ataaaaccgg gcagggtttt tataaaaaag tgaagaacgc ttccggcaag agcgaaattc
     3901 ttgcgctcga tctgaagacg atgcaataca acccgtcgca gaaagtgaaa ttcgcgacgc
     3961 tcgaacagac gaaacccatt gagaacctgc gtgagcgcat gagcgtgctg atcgccggaa
     4021 aagacaaagc gggggaattc taccgcgcgt ctttctttgc cttgttcgct tacgtttcca
     4081 accgcatccc ggagatcacg gatgatcttt acaagatcga ccaggcgatg aacgccggct
     4141 tcggatggga gctgggtccg ttcgaagcgt gggacgcgct tggcgtggcg gaaacagtga
     4201 aagcgatgga agccgcgggg aacaagcctg cgcagtggat ctacgacatg ctttcctcgg
     4261 gcgcatcctc tttttacaga ttcgataatg gcacgaaact gtgctatgac attccttcga
     4321 aaaaatacaa aaccattccc ggcacggaag atttcatcct gctggaaccg ctgcgcggaa
     4381 cgaaaacagt ctggaaaaat tccggcgcta ccgtgaccga tatcggcgac ggcattctca
     4441 acctggaatt ccatacgaag atgaacacga tcggcggcga agtgctgcag ggattgaaca
     4501 ccgcgatcga tatggcggaa aaagatttcc gcggtctcgt catctccaat gaaggcggga
     4561 atttttccgc gggcgccaac ctcggcatgg tgttcatgtt cgccgtggaa caggaatggg
     4621 acgagctcga cttcgctgtg cgtgcgttcc aggctgcgac gggaagagtg cgttattctt
     4681 cgatcccggt tgtggtcgcc ccgcaccaac ttacacttgg cggcggaacg gaaatctgtc
     4741 ttcatgcgga cgccgtcgtc gcacatgcag aaacgtacat gggccttgtg gaattcggcg
     4801 taggcgttat cccgggcgga ggaggaacga aagaatttgc cacgcgtctc gcggatgaat
     4861 tacacgaagg cgacgtcgag ctgaattcct tccgcaaccg tttcctcacg atcgggcagg
     4921 cgaaagtttc cacatcggct tatgaagcat tcgacctcgg ttacctgcgt acccataaag
     4981 accgcgtggt gatgtcgcgc aaccgtttgc tcgctgaagc gaaaatggaa gcggttcgtc
     5041 ttgcggaaga aggttacacc aaacccgtca tgcggcacga catccgcgtg ctcggccggc
     5101 aggcgctcgg acttgcgtac gtcggtgcga attccatgca gagcgggaaa tatatttccg
     5161 aacacgattc gctgatctcg cgcaagctcg cgtgggtgat gtgcggcggc gatttgtcgc
     5221 aacccgcact cgtaagcgag caatacctgc tcgacctcga acgcgaagcg tttttgtcgc
     5281 tgtgcggaga acggaaaaca ctggaaagaa tgcaaagtat tctgacgggg ggaaaagtgc
     5341 tgaggaatta aacagtcagc cctcggtaca tttttcattg cgcttgtcag gctgagcagt
     5401 gaaacatgtc gaagacggca agcgcaatga aaaacactca gtctgactgt aaaatattga
     5461 taccatgaac gcatacatca tagcaggtct tcgctccgca gtaggaaaag ctccgcgcgg
     5521 cttgttccgt tttctccgtc ctgacgatct cgccacacag gtgatccgac aggtcgtggc
     5581 gcaggtgccg aacctcgaca aagaacagat cgacgacgtc attgtcggga atgcgatgcc
     5641 ggaagccgag cagggactca acgtaggccg gctgatctcg ctgctggcgc tcgacacgga
     5701 taaagttccc ggcatgacgg tgaaccgtta ctgttcatcg ggtctcgaga ccatcgcgat
     5761 cgccgcgtcg aaaattcacg ccgggcttgc cgactgcatc atcgccggcg gcgtggaatc
     5821 gatgagcctt attcccatgg gcggctggcg cgtgatcccg aatacggata tcgccgccaa
     5881 ccatcccgac tggtattggg gaatgggcct taccgcggaa gctgtcgcga acgaatacaa
     5941 cgtttcacgc gaggaccagg atgcgtttgc gctttcatcg cacctgaaag cgatcagcgc
     6001 gatcaaagaa ggaaaattca gaagcggcat cgcaccggtg accgtgaagg aaattttcct
     6061 cgacgaaaag aacaagcggc agtcgcgtga atacattgtc gacaccgatg aaggtccgcg
     6121 cgcggatacg aacgcggaag cgctcgcgaa gctgaaaccg gtttttgccg aaggcggcag
     6181 cgtgaccgca gggaattctt cgcaaacatc cgacggtgca gcgtttgtca ttatcgtcag
     6241 cgaaaaaatg ctgaaagcgc tgaacgtgca gccggtcgcg cggctcgtga gctatgctgc
     6301 agccggcgtt cctccgcgca tcatgggcat cggtcctgtg cacgcgattc ctaaagcgct
     6361 ggcgaaagca ggcatgaagc tcggcgacat tgatcttatc gagctgaacg aagcgttcgc
     6421 atcacaatcg atcgcggtgc agcgtgagct gaagatcaac ccggacatcc tcaacgtgaa
     6481 cggaggcgcg atcgcgctgg gacacccgct cggctgcacc ggtgcaaaac ttaccgtgca
     6541 ggtgctgaac gagctcaaag caagaaaaaa gaaatacgga atggtgacga tgtgcgtggg
     6601 aacaggacag ggagcggcgg ggatatttga gatgatgtga aacccgtgat gtgtttacag
     6661 atattcagga aacagcatac acagaaaaag aaaaacaacc tttatcacga gcggcaacat
     6721 cctgcggagc gcagcgggta tttttgaacc ggtcagccaa tgacaaaaag atgaaaacct
     6781 gttttacaaa ttgtgttaac acaaccaata acggtttcca tcgtgacgtt tgtcagcggt
     6841 ttgatctatt cacttcaaaa acaaaaacgc catgactcct ccgcaacaac ctcccgttcc
     6901 acaggtccct ttccagcttc cccagaagcc gaaaaaggaa aatccttcca ttaccgtaaa
     6961 gctgctcgtc attgcattgc tcgtcctcat cctcgttgtt ccctggggac ttctgcagtg
     7021 gctgatctat gaccgccaga acatgaacga ggaggcggtg aacgaagttt cacagaaatg
     7081 gagcaacccg cagaacgtga gcgggcccgt gctgtatgtt ccgttcatcg cgacgagttc
     7141 cggcggctct gattacactg cctacctgaa agtgcttccg gaagaactca gcatttccgg
     7201 gaaagtcgtt ccggaaaaac gttaccgcgg catttatgaa gtcgtggtat acagctccgc
     7261 cctttccgta accgggaaaa tgaagatccc cgattacgaa gagatgaaga tcgatgcgaa
     7321 gaagatcatg tgggaccggg ccttcgtagg gatcggcgtg gatgacatgc gcggcatacg
     7381 cgatggcgtg aatttcaagt ggcgtgacgc gtcttattcg tgcaatcccg gcatctcgac
     7441 aggaacggac ggttacagct cttccacggg cgtaagctgt cccgttgctt tcgcgcacac
     7501 cgacacggtt cccggcgctg aaatcccgtt ctcgttcgac ctcgacctcg acggaagcaa
     7561 aaacctcaac ttcgagccgc tgggaaaaac aacgaaggtc catcttactt ctacgtgggc
     7621 ttcgccgagt ttttccggcg cattccttcc cgatacgcac aacatcaaca cgaacggttt
     7681 cgtcgccgac tggaaagtgc tgcacctcaa ccgcaattac ccgcaggcgt gggtcgacaa
     7741 tgcgtacaac acggcggatt cgcgtttcgg cgtggagctg atggtgccgg tagatcatta
     7801 cacgaaatca tcacgtgcgt cgaaatacgc catcatgctc atcggtctta cgttcctcat
     7861 ttatttcttc gtggaagtca tgcagaaact gcgcatccac ccgttccagt acattctcgt
     7921 cgggctcagc atctgcgtgt tctacctgct gctgctgtcg ttctccgagc acatggtgtt
     7981 caacttcgcc tactggaccg ccgcttcgct tacgctgctc gcgatcactt cgtattcggg
     8041 cgccattttc cgtaaaaaat taccgaccgc cgtcatggga acttccctgc tgctgatgta
     8101 cgggtttatg ttcgtcatca tccagctgga agattacgcc ctgctcgtcg gttccgtcgc
     8161 actgttcctg atcctgatcg tactgatgta tctttcgaga aagatcaaat ggtacggtga
     8221 cgacgaaaac aatccaacgt ctaccactta aaaaacgctt tttatgagca atgccatcaa
     8281 aggaggagaa ttcctcatcc gcgaaacgaa cgccgcagac attttcattc ccgaagaatg
     8341 ggatgaagaa tcgaagatga tcgcacagtc gtgcaacgat ttcctccagc aggaagtctt
     8401 cccgaacctc gaccgcatcg atcagcagga agaaggactg atggtttcac tgctcgacaa
     8461 agccgctgcg ctcggactcc tcggcatttc ggtgcccgag gagtacgggg gcttcggaaa
     8521 agatttcaaa acgtcgatgc tgacaacgga aatgctcggc gcgggacatt ccttcgcagt
     8581 cgccattgcg gcgcacacgg ggatcgggac gctgcccatc ctgtattacg gcaacgatgc
     8641 gcagaagaaa aaatacattc cgaagctggc gaacggggaa tggaaagcgt gctactgcct
     8701 caccgagccg ggcgcaggtt ccgatgcgaa ttccgggaag acgaaagcgg tgctttcacc
     8761 cgacggaaaa cattatgtga tcaacgggca gaaaatgtgg atcacgaatt caggcttcgc
     8821 agaaattttc atcgtgttcg cgaagattga cgacgacgaa aacctcagcg cgttcatcgt
     8881 ggaaaaaagc ttcggcggtg ttacgctgaa cccggaagag cacaagatgg gcatcaaagg
     8941 cagctccacg cgccaggtgt ttttcaacga ctgcaaagtt cccgtggaaa atcttttgtc
     9001 ggaacgcggc aacgggttca ggatcgcggt gaacatcctc aacgtgggcc gcatgaagct
     9061 cgcgggcgcg gcactgggcg catcgaaacg cgtttgttcc gtcgcaacac agtacgcgaa
     9121 tgagcggcag cagttcgggc ggcccatcgc aaaatacggc gcgatccgcc acaagcttgc
     9181 agaaatggcc atccgcattt acgcgctcga gtcgacgctc taccgcgcga cgcaaagcat
     9241 cgacgacgcg atcaaagccc tgcaggccgg cggaatggag ttcgaaaaag cgaagctgaa
     9301 aggcaccgag cagttcgccg cggaagccgc gatactgaaa gtgaacggca gcgaagtgct
     9361 gaactacgcc gtcgatgagg gcgtgcagat ctacggcggc atgggctatt cagccgaagc
     9421 gccgatggaa cgcgcgtacc gcgatgcgcg catcaaccgc attttcgaag gcacgaacga
     9481 gatcaaccgc atgctcattg tcgacatgct gctgaaacgc gcgatgaaag gtgagctcga
     9541 cctcatgggt cctgcgcaga aagtcgcagg cgagctgatg gcgatccccg atttcggcgc
     9601 gccggacacg acgctcttcg ccgccgagaa aaaaacgttg cagcagttca aaaaagcgat
     9661 cctgcttgtc gcgggcgcgg ccgtgcaaaa gctgatgatg cagctcgcca aggagcagga
     9721 agtgctgatg aacatttccg acatgctcat cgacctgtac acagcggaat cactgcagct
     9781 gcgcgtcgaa aaactcgtca gcaggaacgg cgaagagaat tgcgccgtgc agctcgacat
     9841 cctgcgcaca ttcatctacg acgccgcgga ccgcatccac aaagcgggga aagacgcgat
     9901 caacagcttc gccgaaggcg acgagcagcg catgatgctg atgggattgc gacgcttcac
     9961 aaaagtcgag gtattcaatt cgaaagaatc gcgcaggcgg atcgccgcga agatgctgga
    10021 ggaaaacaag tattgctggt aagcgctttc gcaggttgcg cgggcaggca gaagagcgat
    10081 cagtttttcc agtactctct tccgtaaatg tcgatgaagc cttcgttgta aataaaaaac
    10141 agccgggaat gagttgttgc ctgcagcggc gctgaataac gcggcggcgt gatgaccgtt
    10201 ccgtctgcgc ggagcagtcc gaatttcccg tcgcgtttgt agcaaaaata tcccggatcc
    10261 agttccacca gctcatcata cacgaacgga acgatcactt tcccgctctt atcggtcaac
    10321 ccgtattttc cgttcactgc acgaaggagc aaaccggatt tcagttccgg gtaatacacc
    10381 tggctgtaca gcggcgggtc caccgggttt ccatggaaat cgaaatacag catactgccg
    10441 gttgaatcga tcgcaatgag ccttgttgaa tccgttacgt cggtggagat acgccggtaa
    10501 accttcggaa gtatcacgcg gcccgtgctg tccatcaatc catacagtcg ctggtctttc
    10561 ccgtaatacg cgccggcatc gccgaaaata aaattatcca tgcgcctgtc gaaccgggtg
    10621 agatacgccg ccctgccgga aatcccgtcg agtttttttc ccgacatcgt gtacacgtcg
    10681 atgagcttgt ccttgtgccg gatcatgaac acctcgctgt cgccggaaga aggacggcgg
    10741 tagcggatgt aattatcata atagaaggga tagatgtaat cgtactcgtc aagtgtcccg
    10801 tcgtaatcgc cggtgatcag cacctggcct ttgctgtctg caaatacgtt accgattttg
    10861 tacacgggac gctccatctc gcgccagccg atgaccggtg gtttgactct ttcatttccc
    10921 acctgtcgcc ccgcagaatc aaacaggaac cagtagaacc gcgtataggc agcgtaaccg
    10981 tctccgcttg cacagacagc gaggaacgta tccggaacaa tgagcttacc gctgcctgaa
    11041 acaacaccgt gcccgtgcct tggattgacg acgagataac cgtcgcggcc gggatcccag
    11101 cccgggtaat tatgccagat catgtacagc gaatcatatt gtcccggcgg aaacaggaga
    11161 tcaccttcgc gtgaaagcag gttgtttgtg ttccgcttat tattggagtt gtcattcact
    11221 tccgcaaaat ttccgcagaa aggacgaatg aaatcaaacc tcgcttccgt gagttttttt
    11281 cccgtggaat ccatcagccc ggtcaggccg ttttcccaaa atatgaattc gctatactgc
    11341 tcgtcgtaag caagcgcgtc atacaggaaa ggaacgaccg tgcggccgga ataatcaacg
    11401 cagccgaacc gttcatcctt cacgcagacg atgaaccggt cgtacaccac tatccttctc
    11461 agcgaatccg tgcccaggtc cgcaatctc
//
LOCUS       DASTSF010000270        12541 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1813974,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000270 DASTSF010000000
VERSION     DASTSF010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 12541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12541
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1813974"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1084)
                     /gene="nusA"
                     /locus_tag="VFU15_07800"
     CDS             complement(<1..1084)
                     /gene="nusA"
                     /locus_tag="VFU15_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016777358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HEU4717721.1"
                     /translation="MENLNLIESFSEFKETKNIDRAMLMSILEDVFRSLIRKKYGSDE
                     NFDFIVNPERGDLEIWRNREIVDDEFAEDSFDYDPNKHISLSDARKIEPDFEIGEQVT
                     DRIMLDDFGRRSVLAIRQNLVSRVLDLEKDAIYRKYKERVGDVITGEVYQVWKKETMV
                     LDDEGNELILPKSEQIPGDFFKKGDTVRAVVSKVDMRNNTPVIILSRTSPSFLEKLFE
                     MEVPEIYDGLITIKKIVREPGERAKVAVESYDDRIDPVGACVGMKGSRIHGIVRELRN
                     ENIDVINYTANSQLLIQRALSPAKITSIKLDEESKRAEVYLKPDQVSLAIGKGGHNIR
                     LAGRLTGYEIDVYRDTDEDVEDVKLDE"
     gene            complement(1109..1588)
                     /gene="rimP"
                     /locus_tag="VFU15_07805"
     CDS             complement(1109..1588)
                     /gene="rimP"
                     /locus_tag="VFU15_07805"
                     /inference="COORDINATES: protein motif:HMM:NF002531.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly cofactor RimP"
                     /protein_id="HEU4717722.1"
                     /translation="MIARDTILQLANDWLANPDSNRNGSYLVDVGVSAGNKITVVLDN
                     LEGTSISECVDMSRWIEKHLDRDAEDFSLEVTSPGLDQPFRVFRQYEKNLGREVEVKL
                     MNGQKVNGKLVRAAAEHIEVEQSTHERIEGKKGRHLVTKSISLPFSEIKETRIVIKF"
     gene            1830..2141
                     /locus_tag="VFU15_07810"
     CDS             1830..2141
                     /locus_tag="VFU15_07810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717723.1"
                     /translation="MKPEKGKRVGNLWKGFLFAVLLLQGNVLLADESSQQTSDSSTGT
                     TAGEILLCIAGIVVIILIAWFFGGGQSKNADIHHAPHGQRRHFDHMNDPHFRKLRKKT
                     S"
     gene            complement(2203..4071)
                     /locus_tag="VFU15_07815"
     CDS             complement(2203..4071)
                     /locus_tag="VFU15_07815"
                     /inference="COORDINATES: protein motif:HMM:NF019284.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717724.1"
                     /translation="MKRILLFVFLFSYFFAHAQVVPVYQDDDPKSKKTYKPKSPKPAK
                     VITYYDDKTKKQKKSEEYWLNGKKQGKCYYYYPDGKMEKNGAYKMDKEDSLWTFYYAN
                     GNKKAEEHYFEGTRSGPAQYWYNSGQVALTCKYAENLPDSVWTTYYENGQVKSIETYS
                     VAMVGFNLVSYKNGHFKYFNDNGKPNSEGYYVNDTLNGTFTTWFPNGTKKAEGNFSKG
                     VKSGKWMEWNDKGTLLLESDYRDSTAYIMNMWSEDGKQVVKDGYGKITLVYDNGATRC
                     IGLYRGGLKDSAWTFWKDNGKLDFRDEYKGGKRNGKSVHWYDDGKVSIDGEYADDKKT
                     GTWTWYKDSGKLYMQGEFSDDMRNGHWQYWFDDKDTLEEEGWYKNDKEDSLWNFWYPG
                     GQKYKTGTFKDGKKNGFWTFWFENGQKLQEGNYVDNLNDGDWISWYDNGQVKDQGSFA
                     KALMIGKWEGWYYNGKQNYSGNYKDGVKEGQWTYWWDNGKLHEKGKYRDGRKEGQWTT
                     WFVTGFVDQTGGYHLDKKDGRWKFFYETGSVKTVTVYKMGNLCGKTVNYYVEGNKESI
                     TRNKIYKQKDYVLTKANGKKVYKKRDVSKPNGTWIYYDTDGKVLYRVKYKKGVKVG"
     gene            complement(4224..5375)
                     /locus_tag="VFU15_07820"
     CDS             complement(4224..5375)
                     /locus_tag="VFU15_07820"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEU4717725.1"
                     /translation="MSALPVYLDYNSTAPVDERVLDAMLPFFRKQFGNAASRTHAYGW
                     AAAKAVDIARAQVAHLLGAEENEIIFTSGSTEAINLAIKGACAAYATKGKHILVSATE
                     HKAVLDTCSFLERAGAEITRIPVDREGIIDLDFLKKNIRRDTILVCVMTANNETGTIQ
                     PVDEIAAIVHEAGSVFFSDTTQALGKMNVHVNEAGIDLCCISAHKICGPKGAGALFVR
                     RKDPRVSLVAQMHGGGHERGLRSGTLNVPGIVGLGKAAEIAAAEGWDDAQRLSVLRTE
                     LEQKIISCGTVFVNGSVKDRLPNTTNLRITGVKAAELIAKLPLLAFSAGSACTSAIPE
                     PSHVLKAMGLSGEESHASIRLSIGKFTTDEEIAKAGELLCNQINALRYS"
     gene            complement(5509..6579)
                     /locus_tag="VFU15_07825"
     CDS             complement(5509..6579)
                     /locus_tag="VFU15_07825"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HEU4717726.1"
                     /translation="MKNLKTILILAALLPLQVKSQTCSGDYQWLVNGLGVSFYGNTTP
                     DITNVIWDFGDGNWDNTNSLNTSHTYAAPGIYHACIQYYNTAQGCGDSTCHTIVIDSC
                     YGSMNYSVNGLTVQFTGSASAGGPNTVYAWDFGDSNSSYAQNPSHTYASAGTYTVCFA
                     FYDSSSGCADSVCEPVSVGGGCYAQFTSVDSMGYAFFVNQSSPGSAGMYVWYFGDGGY
                     STAYNPSYQYSYPGTYQVCLEAFDSLQNFCDSTCHYITVNNVSSVNEETIVRNSLLLS
                     PNPADEDLRISWNQAADADVQIAVLDLAGRTVITPVMVRGGRGVNTKEISTRGLAAGM
                     YMLRIDAGNQTGNSPLVVTHKE"
     gene            6713..7603
                     /locus_tag="VFU15_07830"
     CDS             6713..7603
                     /locus_tag="VFU15_07830"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEU4717727.1"
                     /translation="MTPGRRNYFLLHFIVFIWGWTAILGKQITLPAIKLVWLRIPIAL
                     GGILAYLLIARKPVAEKPSNLVKYFGVGLIVALHWICFYASIKESNVSITLACFSTGS
                     LFTSFIEPVFFKRKISLYEIIFGLMVVIALLIIFGVETHFSIGIVLGVLAALTSSFFG
                     VVNGYMVKKGHDGTLISLYEMAGGFAGMTVYVLLFHPFPGPLLAMSGEDLFYMLLLGL
                     VCTSLPFLISLYILKTISPYTVSLTLNLETVYGIIFAYFIFHEDKQLTPWFYLGAAII
                     LSTVFLNGWIKMRKPGNAAS"
     gene            complement(7625..8920)
                     /locus_tag="VFU15_07835"
     CDS             complement(7625..8920)
                     /locus_tag="VFU15_07835"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HEU4717728.1"
                     /translation="MKKLFLFLAVCFTYARAQAACQAAFTYTQSAGTFNFTDASTTTS
                     GSVSQWLWTFGDNSLPSSQQNPSHTYAACGYYIVTLNIFTSSFCTSSISDTVLVTSGF
                     TGSFTATVDTTTGNVQFNAQPASSHAIYNWDFGDASTGTGVAPTHLYAPGTYTVCVAM
                     ADTDGICSDTVCNVITVYVAPTNCSATFTNTPIGTGNESFTATPFNLGWTYSWDYGDS
                     NTGNGFFSTHQYLASGTYTVCLTVTDSANNCTSNFCDTVNIAFPTSCPPSFTANGLNG
                     TYIFTASPLSIQNTYSWDFGDGSPAGSGVVANHTYTTAGTYTVCLTMTTQGGCVGVTC
                     DTITVVTGVDESSLLPGSALFPNPADQDIMVSYTLSSSANVQLQVFDLSGRVLWSADK
                     GSQAGAQRSDVDVSSLAPGVYFFGITANGQRTILKFVKS"
     gene            9392..11347
                     /gene="ispG"
                     /locus_tag="VFU15_07840"
     CDS             9392..11347
                     /gene="ispG"
                     /locus_tag="VFU15_07840"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007658562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(E)-4-hydroxy-3-methylbut-2-enyl-diphosphate
                     synthase"
                     /protein_id="HEU4717729.1"
                     /translation="MQSLDPYCASMTEYVRWKTRVVNIGDVPLGGDLPIRVQSMTTTD
                     TLDTKATVEQSIRMIEAGCEYVRITAPSLKEAQNLESIKKELRNRGYKTPLIADIHFT
                     PNAAELAARIVEKVRVNPGNYADKKKFEHIDYSDAEYNSELERIRTRFLPLVKVCKEY
                     GTAMRIGTNHGSLSDRILSRYGDTPLGMVESAMEFLRICEAENYRDIVLSMKASNPQV
                     MIQAYRLLVQHMMQAGMNYPLHLGVTEAGDGEDGRIKSAAGIGTLLEDGLGDTVRVSL
                     TEEPEAEIPVAKMLVDRYAKRSGHHAITAIETREGKIVLPYSPYDYSRRKTESVANVG
                     GHNVPRVVADLAGKEKLTRASLFPAGYHYSVPLDKWNITDQACDLIYTGDNDIDFEIP
                     GTLGVILNSEKWKTLENTSQRYPVFCGHELFEAEKRSAVMNFVAADIKTLTEEFIQKI
                     STDKTAVIVLHTTNEHAMPELRRAFMLLEESNCETPVIIKRKYVSEGEDAFRLFSATD
                     TGGLLLDGMGDGTWLSSKSGLAMNVVNSTAFGILQATRTRISKTEYISCPSCGRTLFD
                     LQETTNKIRKRTSHLKGVKIGIMGCIVNGPGEMADADYGYVGTGPGKITLYKGKEVVK
                     RNIPENDAVEALVNLIREHGDWVETAS"
     gene            complement(11407..>12541)
                     /locus_tag="VFU15_07845"
     CDS             complement(11407..>12541)
                     /locus_tag="VFU15_07845"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEU4717730.1"
                     /translation="LPTISASASMATVCMGMPDTLTSTGGTTYSWSSGGTSAMEIVTP
                     SSAATYTVTGTDMNGCSGSAMVSVAVNALPVVDLGPDVTQCGGSVTLDAQNAGSTYSW
                     SDASASQMLSVSASGTYYVDVTDANGCMSSDTAMVTINSNPVVNLGPDVTQCGGSVTL
                     DAQNAGSAYSWSDASTTQTISVSVSGTYYVDVTDTNGCTGSDTAMVTINQNPTVSGSA
                     TATSVCVDDASVALTGTPAGGTWSGTGVTGSTFSPAAAGTGTQTATYSYTDANGCSGS
                     GTVTITVNACTGVAENVAANGVNVFPNPNNGTFTVAVNANVNELVIVITDLQGRVVYS
                     SADAGVQAGFSKQVSLGAQASGLYFMQISADGRQVTDKIAVEK"
BASE COUNT         2773 a         3435 c         3241 g         3092 t
ORIGIN      
        1 actcgtcgag cttcacgtct tccacgtctt catctgtatc gcggtaaacg tcgatctcgt
       61 aaccggtcag ccgcccggcg agacggatgt tgtgtccgcc cttgccgatg gccagcgaaa
      121 cctggtcggg cttgaggtaa acttccgcgc gtttgctttc ttcatccagc ttgatggaag
      181 tgatcttcgc gggactgagc gcacgctgga taagcagctg cgaattggcc gtgtaattga
      241 tcacgtcgat attctcgttg cgcagctcgc ggacgatgcc gtggatgcgg gaacctttca
      301 tccccacgca ggcgccaacc gggtcgatgc ggtcgtcgta agattccacc gccacctttg
      361 cgcgttcgcc gggttcgcgt acgattttct tgatcgtgat aagcccgtcg tagatctccg
      421 gaacttccat ttcgaaaagc ttttcgagga aagaaggaga cgtgcgggaa agaatgatga
      481 ccggcgtgtt gttgcgcata tcgactttgg aaacgacagc gcggaccgtg tcgccttttt
      541 tgaagaagtc gcccgggatc tgctcgctct tgggaagaat aagttcgttc ccttcatcgt
      601 cgagcaccat ggtttctttt ttccacacct ggtaaacttc gccggtgatg acatcgccaa
      661 cgcgctcctt gtatttgcgg tagatggcgt ccttctcgag atcgagcacg cgggaaacga
      721 ggttctggcg gatcgcgagc accgagcggc gcccgaaatc atcgagcatg atgcggtcgg
      781 tgacctgctc gcctatttcg aaatcgggtt cgatcttgcg cgcatcggaa aggctgatgt
      841 gcttgttggg atcgtaatca aagctgtctt cggcgaattc gtcatccacg atctcgcggt
      901 tgcgccagat ctcgaggtcg ccccgttcag ggttgacgat gaaatcgaag ttttcatcac
      961 ttccgtattt tttacggatc aggcttctga atacatcttc gagaatgctc atgagcatgg
     1021 ccctgtcgat gttcttggtc tctttgaatt ccgagaagga ttcgatgagg ttcaggtttt
     1081 ccatttttac agttggtaga tcggattttc agaatttgat tacgattcgt gtttctttta
     1141 tttcggaaaa aggaagcgag atgcttttgg tgacaaggtg tcttcctttt tttccttcaa
     1201 tgcgttcatg cgtgctttgt tcgacctcga tatgttcagc tgccgcacgc accagctttc
     1261 cgttcacttt ctgcccgttc atcagtttca cttccacttc acggccgaga tttttttcat
     1321 actgccggaa tacgcggaac ggctgatcga gaccggggga agtgacttcc agcgagaagt
     1381 cttccgcatc cctgtcgaga tgcttctcta tccaacggct catgtccacg cattcgctga
     1441 tagaagtgcc ttcgaggttg tcgaggacga ccgttatctt attgcctgcg cttacgccga
     1501 catcgaccag gtagcttccg tttcggttgc tatccgggtt tgcgagccaa tcgttcgcga
     1561 gctgcagaat ggtatccctg gcaatcattg aaaaaaacag gggactctcg tcccctgatc
     1621 tatacgtact tactaacagc gtgcaaatat aaacaggttt ttgttgaaaa acaagtgcgt
     1681 ttaacgaaac attaacagga caaatgcgta aatatctcag gcaaacgtct tatttttgtc
     1741 gacgaattcg ttctttaaga gtatatgctt ttgttctccc caaagggcat gcatagatag
     1801 tttgtttaaa acccataaaa ctacttatca tgaaaccgga aaaaggaaag agggtgggga
     1861 acctctggaa agggttcctt tttgcagttc ttttactgca gggaaatgtt ctactcgctg
     1921 atgaatcgtc ccagcaaacc agcgatagca gcaccggcac cactgccggc gaaattctgc
     1981 tgtgcattgc ggggattgtg gtcataatac tgatcgcatg gttcttcgga ggaggacaat
     2041 ccaagaatgc cgacatacat catgcaccgc atggtcaaag gcgccacttc gatcacatga
     2101 acgacccgca tttcagaaag cttagaaaga aaacctcatg agtccgacgt gaacaaaccc
     2161 atgaaaagtc ccgcagcgat gcgggatttt tttttgcccg ggtcagccga ctttcacgcc
     2221 cttcttatac ttaacacggt aaagcacctt cccatccgta tcgtaataga tccacgtacc
     2281 gttcggcttg ctgacatcgc gtttcttgta caccttcttc ccgttcgctt ttgtaagcac
     2341 ataatccttc tgcttgtata tcttattccg cgtaatgctt tccttgttgc cttccacgta
     2401 ataattgacc gtcttgccgc agaggtttcc cattttataa acggtgaccg ttttcacgct
     2461 cccggtttcg tagaaaaact tccagcgccc gtctttttta tcgagatgat agccgccggt
     2521 ctggtcgaca aagcctgtta caaaccaagt ggtccactgg ccttccttcc gcccgtccct
     2581 gtatttgcct ttttcgtgca atttgccgtt atcccaccag tacgtccact gaccttcctt
     2641 cacaccgtcc ttgtaattcc cggaataatt ctgcttgccg ttgtaatacc agccttccca
     2701 tttcccgatc atcagtgctt ttgcaaatga gccctggtcc ttgacctgcc cgttgtcgta
     2761 ccacgagatc cagtcgccgt cgttcaggtt gtcgacgtaa ttcccttcct ggagcttctg
     2821 cccgttctcg aaccagaaag tccagaagcc gttcttcttg ccgtctttga acgttcccgt
     2881 tttgtatttc tgcccgccgg gataccagaa attccagagg ctgtcttcct tgtcgttttt
     2941 gtaccagcct tcttcctcga gcgtgtcctt gtcatcgaac cagtattgcc agtggccgtt
     3001 acgcatgtcg tcgctgaact cgccctgcat atataatttt ccgctgtcct tgtaccatgt
     3061 ccacgtgccg gtttttttgt cgtcggcgta ttcgccgtcg atgctcactt tcccgtcatc
     3121 gtaccagtga acggatttcc cgttgcgctt accgcctttg tattcgtccc tgaagtcgag
     3181 cttcccgttg tctttccaga acgtccatgc gctgtctttc agtccgccgc ggtaaagccc
     3241 gatacagcgc gtagcgccgt tgtcgtacac cagcgttatt tttccgtagc catctttcac
     3301 cacctgcttt ccgtcctcgc tccacatgtt catgatgtag gccgtgctgt cgcggtaatc
     3361 gctttcgagg agaagcgttc ccttgtcgtt ccactccatc cacttgccgc tcttcacgcc
     3421 tttcgaaaaa ttcccttcgg cttttttggt gccgttcgga aaccacgtgg tgaacgttcc
     3481 gttcagcgta tcgttgacat aataaccttc gctgttcggt ttcccgttat cgttgaaata
     3541 tttgaaatgc ccgttcttgt atgatacgag gttgaagccg accattgcaa cggaataggt
     3601 ttcgatgctt ttcacctggc cgttctcgta gtaggtcgtc cacacgctgt cgggaagatt
     3661 ttccgcgtac ttgcacgtaa gagcgacctg gccgctgttg taccaatact gcgccgggcc
     3721 gcttcgcgtt ccttcgaaat aatgttcttc cgcttttttg ttgccgttgg cgtaatagaa
     3781 tgtccagaga ctgtcctcct tgtccatctt gtacgccccg ttcttttcca ttttcccgtc
     3841 gggataatag taatagcatt tgccctgctt tttcccgttg agccagtact cctcgctttt
     3901 cttctgcttt tttgttttat cgtcgtaata ggtaatgacc ttcgccggct tgggtgattt
     3961 gggcttgtag gttttcttcg atttcggatc atcatcctgg taaaccggga cgacctgcgc
     4021 atgcgcaaaa aagtaagaaa acaggaaaac gaacaggaga atgcgcttca tgtagtacta
     4081 acggatcaat gcggtgattt ctttttcggg atttacaaaa ctatctgaaa tgtttccgcc
     4141 gggtcagcgt tttatcagga gaaatgaaca acaggccgtt gatgcgggcc ggtatccgcc
     4201 ggtaaaacag ggtagttcct ccgtcagcta taacgcagcg cgttgatttg gttacagagc
     4261 agctctcccg ctttcgcgat ctcttcatcc gtcgtgaatt ttccaatgct gagacggatg
     4321 gaggcatgcg attcctcccc ggagaggccc atcgctttca gcacatgtga aggttccgga
     4381 atggctgatg tgcacgcaga gcctgccgaa aaggcgagca acggcagctt cgcgatcagc
     4441 tcagcagctt ttaccccggt tatgcgaaga ttcgtcgtat tgggcaagcg gtctttcacg
     4501 cttccgttca cgaaaacagt tccgcagctt attattttct gctcgagctc cgttcgaaga
     4561 accgaaagcc gctgcgcatc gtcccatcct tccgctgccg cgatctccgc cgctttgccg
     4621 agtcccacga taccgggaac attgagcgtt cccgaacgca accctctttc atgaccgccg
     4681 ccgtgcatct gcgccaccag cgaaacgcgc ggatcttttc tccgcacgaa caatgcgcct
     4741 gcacctttcg gtccgcagat cttgtgcgcg gaaatgcagc acaggtcgat cccggcttca
     4801 ttcacgtgaa cattcatttt tcccagggcc tgcgttgtat cgctgaaaaa aacagaaccc
     4861 gcctcgtgca cgatcgcggc gatctcatcc accggctgta tcgtccccgt ttcattgttc
     4921 gccgtcatga cgcagaccag tatcgtatcg cggcggatat tttttttcag aaaatcaaga
     4981 tcgatgatgc cttcgcggtc gaccgggata cgggtgatct ccgcacctgc gcgctcgagg
     5041 aagctgcacg tatcgagcac cgctttgtgt tccgtggccg acaccaggat gtgctttcct
     5101 ttcgttgcat aagcggcgca ggctcctttg atcgcgaggt tgatcgcttc ggtggagccg
     5161 gaagtgaaaa tgatctcgtt ttcttccgca ccgagaagat gcgccacctg cgcgcgtgcg
     5221 atatccacgg ctttcgctgc cgcccacccg tatgcatgcg tgcggctggc cgcgttgccg
     5281 aactgtttcc tgaaaaaagg cagcatcgca tccagcacac gttcatccac cggcgcggtg
     5341 gaattgtaat cgagataaac cgggagcgcg gacatgcctg caaagatacg cttcaccgga
     5401 ggcgtaaagg gtaaaaaaag aaggacgccg tctcccgtcg gcgtcctccc ggaatttccc
     5461 ccagaaacac acgaaaattg ggggaggagg caagatgaat tgtcggtgtt attccttgtg
     5521 cgtcacgacg agcgggctgt tcccggtctg gtttcccgca tcgatgcgaa gcatatacat
     5581 tcccgctgca agtccgcggg ttgaaatctc tttcgtgttg acgcctcttc cgccgcggac
     5641 catcaccggt gtgatcaccg ttcttccggc aaggtcgagg accgctatct gtacatctgc
     5701 atcggctgcc tggttccacg atatacggag atcttcatcc gccggattgg gcgacagcag
     5761 caatgaattc cgcacgatag tttcttcgtt cacggaagac acattattga cagtgatgta
     5821 atgacacgtg gaatcgcaga agttctgcag cgaatcaaaa gcctcgaggc aaacctggta
     5881 ggtaccggga tatgaatact ggtaagaagg attgtacgcg gtggaatatc cgccatcgcc
     5941 aaaataccag acatacattc cggcactgcc cggcgaagac tggttgacga agaacgcata
     6001 gcccatggaa tcaaccgagg tgaactgcgc gtaacatcct cctccgacgg agaccggctc
     6061 gcagacgctg tcggcacagc ctgacgagct atcgtaaaac gcgaaacaaa ccgtgtacgt
     6121 tcccgcggat gcatacgtat gcgacgggtt ctgcgcatac gaagaattgg agtcgccgaa
     6181 atcccatgcg taaaccgtgt tcggtccgcc cgcgctcgcg gatcctgtaa actgcaccgt
     6241 gagcccgttg acggaataat tcatcgagcc gtagcaggaa tcgatcacga tcgtatggca
     6301 tgtgctgtcg ccgcatccct gtgcagtatt gtaatactgg atgcaggcgt ggtagatccc
     6361 gggagcggca taggtgtgag acgtgttgag tgaattcgta ttgtcccagt tcccgtcgcc
     6421 gaaatcccag atcacattgg tgatatcggg tgtcgtgttg ccataaaacg aaaccccgag
     6481 gccgttgacc agccactggt agtctccgct gcatgtctgc gacttcacct gcaaaggcag
     6541 gagggccgcg agaatgagta tggttttcag gtttttcatg aggcagatac cggtacaaaa
     6601 atgaaacagg tttttttttt caccaaatca actttaaaaa atcttacctg tccagcgtta
     6661 catgcggccg gggatgaatt agcgtaactt tggcccgctc aatccgcttt ttgtgacgcc
     6721 aggccgcagg aactattttc tacttcattt tattgtattt atctggggat ggacggccat
     6781 actcggcaag cagatcacgc tgccggcgat caagctggtc tggctgcgga taccgatcgc
     6841 cctcggaggc attcttgctt acctgctcat tgcccggaaa cccgtcgccg aaaagccgtc
     6901 gaacctcgtg aaatatttcg gtgtgggcct tatcgtggcg ctgcactgga tctgctttta
     6961 cgcctccatc aaggaaagca acgtttccat cacgctggcg tgcttttcca ccgggtcgct
     7021 gttcacatcg ttcatcgagc ccgttttttt caaacggaaa ataagcctct acgaaatcat
     7081 tttcggcttg atggtcgtga tcgcgctgct gatcattttc ggcgtggaaa cgcatttttc
     7141 catcggcatt gtgctcggcg tgctggctgc gctcacttct tcttttttcg gcgtggtaaa
     7201 tggctacatg gtaaaaaaag gccacgacgg aacgctgatc tcgctctatg aaatggccgg
     7261 gggctttgcg gggatgaccg tttacgtcct gctttttcat ccttttccag gacccctgct
     7321 ggcgatgtcg ggggaagatc ttttttacat gctgctgctc ggcctcgtat gcacatcgct
     7381 gccgttcctt atcagccttt acatccttaa gaccatttcg ccctataccg tttcacttac
     7441 gctgaacctg gaaacggtgt acgggatcat tttcgcttac ttcattttcc acgaagacaa
     7501 gcagctcacg ccctggtttt acctcggcgc ggccatcatc ctttccaccg ttttcctcaa
     7561 cggctggata aaaatgcgca agccgggaaa tgccgcttcg tgaaaaaagc cacgggtgat
     7621 cgtctcagct cttcacgaat ttcaggatgg tccgctgccc gttcgcggtg atgccgaaga
     7681 aataaacgcc cggcgccagc gaagaaacat ctacatccga acgttgcgcg ccggcctgcg
     7741 aacctttatc cgccgaccac aggacgcgtc ccgaaagatc gaatacctgc agctgcacat
     7801 tcgcagaaga agacaacgtg tagctgacca ttatgtcctg gtccgcggga ttggggaaaa
     7861 gcgcggaacc cggcaaaagg ctgctttcgt ccacgccagt taccaccgtg atcgtatcgc
     7921 acgttactcc cacgcaaccg ccctgtgtcg tcatcgtgag gcagaccgta tatgttcctg
     7981 ccgtcgtgta cgtatgattg gccacaacac cgcttcctgc aggtgaaccg tcaccgaaat
     8041 cccaggaata ggtgttctgt atgctcagcg gcgatgcagt gaaaatgtac gttccgttca
     8101 gtccgttcgc agtgaacgat ggcggacatg aagtaggaaa agcgatgttc accgtatcgc
     8161 agaaattgct cgtgcagttg ttggcggaat cggtgacagt gaggcaaacc gtgtacgttc
     8221 ccgaagcaag gtactggtgc gtggagaaaa acccattccc ggtgttgctg tcgccgtaat
     8281 cccaggaata cgtccacccg agattgaaag gtgttgcggt gaacgattca tttcccgttc
     8341 cgatcggtgt gttggtgaac gtagcgctgc agttcgtcgg cgcaacgtaa acagtgatca
     8401 cgttgcagac ggtgtcggaa cagattccgt cggtatcggc catcgccacg catacggtgt
     8461 acgtgccggg cgcatacagg tgcgtgggcg ctacgcctgt tcccgtcgat gcgtcgccga
     8521 aatcccagtt atagatggcg tgcgaagaag ccggctgcgc gttgaactgc acattgcccg
     8581 tggtcgtgtc gaccgttgcg gtgaacgaac cggtgaaacc cgaagtgacg agaaccgtgt
     8641 cgctgatgct cgaggtgcag aacgagctgg tgaaaatatt cagcgtaacg atgtagtagc
     8701 cgcaggcagc gtacgtgtgt gaaggattct gctgcgagga cgggagcgaa ttgtcgccga
     8761 atgtccacag ccattgcgaa acgctgccgg atgttgtagt cgacgcatcg gtaaaattga
     8821 atgtgcctgc actctgcgta taggtgaatg cagcctggca ggctgcctgc gcacgggcgt
     8881 acgtaaagca cacggcgagg aagaggaaaa gttttttcat cgggttttgg gttgaagggc
     8941 aaatttacat tcaattcata ttacgggaca attagatcgt tgccgccgga gtttaatgat
     9001 gaaaagagac ccggaaaaac tgtttcccca tgcaatacgc gctgttttga ttcctaccgc
     9061 aggaattcat ctcacatgac ttcttctttg cccttgacgt tggccatttc catcctgtcg
     9121 tcctcgactc gcgctcggac agacagggta ttcagactga tgcggggcct catttctcgt
     9181 ttggagtggg cagactttaa gtcgtaaatc atacctgtcg tcctcgactc gcgctcggac
     9241 agacagggta ttcatactga tgcggggcct catttctcgt ttggagtggg cagactttaa
     9301 attgtacctg tcatgacctg tcgtcctcga ctcgcgcccg ggcaggcagg ggcggttgat
     9361 ttggaagagg actcagtatc tttgtacctg catgcaatca ctcgatccct attgcgccag
     9421 catgacggaa tacgtccgct ggaaaacccg cgtggtgaac atcggcgatg tgccgctcgg
     9481 tggtgacctt cccatccgcg tgcagtcgat gacaaccacc gacacgctcg acacgaaagc
     9541 gacggtggag caatccatcc gcatgatcga ggccggctgc gaatacgtgc gcatcacagc
     9601 gccaagcctg aaggaagcgc agaacctgga gagcatcaaa aaagagcttc gtaaccgcgg
     9661 ttacaaaacg ccgctcatcg ccgatatcca tttcaccccg aacgccgccg aactggctgc
     9721 gcgcatcgtg gagaaagtgc gcgtgaatcc cggcaattac gccgacaaga aaaaattcga
     9781 gcacatcgat tattccgatg cggaatataa ttccgagctc gagcgcattc gaacacgttt
     9841 cctcccgctg gtaaaagttt gcaaagaata cggaacagcg atgcgcatcg gcacgaacca
     9901 cggttcactg agcgaccgca ttctctcccg ttacggcgat acaccgctgg gcatggtgga
     9961 gagcgctatg gaattcctgc gcatctgcga agctgaaaat taccgcgaca tcgtgctttc
    10021 catgaaagcg agcaacccgc aggtgatgat ccaggcgtac cgcctgctcg tgcagcacat
    10081 gatgcaggcc ggaatgaatt acccgctgca tctcggtgta acggaagccg gcgacgggga
    10141 agacggaaga ataaaatccg ccgccgggat cggcacgctg ctcgaagacg gactcggcga
    10201 cactgtgcgg gtttcgctga ccgaagaacc ggaagcggaa attccggtag cgaaaatgct
    10261 cgttgaccgt tatgcgaaac gaagcggtca tcacgcgatc acagcaattg aaacgcgcga
    10321 aggaaagatc gtgctgccgt attctcctta cgattacagc aggcggaaaa cggaaagcgt
    10381 agcgaatgtc ggcggacaca acgttccgcg cgtcgttgca gatcttgccg ggaaggaaaa
    10441 gcttacacgg gcgtcccttt ttcccgccgg ctaccactac tccgttccgc tcgacaaatg
    10501 gaacatcacc gaccaggcct gcgatcttat ttacacgggc gataacgaca tcgatttcga
    10561 gatccccggc acgctcggcg ttatcctcaa cagcgaaaaa tggaaaacgc ttgaaaatac
    10621 atcgcagcgt tatcctgttt tctgcgggca cgagcttttc gaagccgaaa aaagatcggc
    10681 ggtgatgaat ttcgttgcgg cggatataaa aacgcttacg gaagaattca tacaaaaaat
    10741 aagcacggac aaaacagccg tgatcgtgct gcacaccacg aatgagcacg ccatgccgga
    10801 gctgcggcgg gcattcatgc tgctcgaaga aagcaattgc gaaaccccgg taatcatcaa
    10861 gcgcaaatac gtttccgaag gagaagatgc gttccgcctg ttcagcgcca ccgacaccgg
    10921 cggactcctg ctggacggca tgggcgacgg aacctggctt tcctcgaaga gcgggctcgc
    10981 gatgaacgtc gtgaattcaa ccgcgttcgg aatactgcag gctacgcgaa cacgcatttc
    11041 aaaaaccgaa tacatctcct gcccttcctg cgggcgcact cttttcgacc tgcaggaaac
    11101 caccaataag atccgcaaac gcacgtcgca cctgaaaggc gtgaagatcg gcatcatggg
    11161 ctgcatcgtg aacgggccgg gtgaaatggc cgatgccgat tacggttacg tcggaacagg
    11221 tccgggaaaa attacgctgt acaaaggcaa agaagtggtg aaacgaaaca ttcccgagaa
    11281 cgatgccgtg gaagcgctgg tgaatctcat ccgcgagcac ggggattggg tggaaacagc
    11341 atcctgaaaa acgggcacaa aaaaacggga gactttgtct cccgttttca taccacacag
    11401 gtagtattat ttctcaacag cgatcttatc cgtgacctgc cgtccgtctg cgctgatctg
    11461 catgaaatac aaaccggaag cctgcgcgcc gaggcttacc tgtttgctga aacccgcctg
    11521 cacgccggca tccgccgaag aataaaccac gcggccctgc aggtccgtga tcacgatgac
    11581 aagctcattc acattggcat tcaccgcaac ggtaaatgtt ccgttgttcg ggttcgggaa
    11641 tacgttaacg ccatttgccg cgacattttc agcgacgccg gtgcatgcgt tgaccgtgat
    11701 cgtcaccgtt ccgcttccgg aacatccgtt cgcatcggtg tagctgtaag ttgcggtttg
    11761 cgttcctgtt ccggcagctg ccggtgagaa cgtcgacccg gttacacccg tgccggacca
    11821 tgttccgcct gcaggtgttc ctgtcaatgc aacagatgca tcgtccacgc aaacggatgt
    11881 tgccgttgcg cttcccgaca ctgtcgggtt ctggttgatc gtgaccatcg ccgtatcgct
    11941 gcccgtgcag ccgtttgtat ccgtcacatc cacataatac gttccggaaa cagaaacgga
    12001 gattgtctgc gtcgtggaag cgtcgctcca ggaataagcg gaacctgcat tctgcgcatc
    12061 aagcgtaact gagccgccac attgcgtaac gtccggaccg agatttacaa ccgggttgga
    12121 attgatcgtc accatggccg tgtcgctgct catgcaaccg ttcgcatccg ttacatcgac
    12181 gtaatacgtt ccggaggcag aaacagaaag catctgtgaa gccgacgcat cgctccagga
    12241 ataagtggag cctgcgttct gcgcatccag cgttaccgaa ccgccgcatt gcgtaacatc
    12301 cggaccgaga tcaacaacgg gcagcgcatt tactgctacg cttaccatcg ccgaaccgga
    12361 acatccgttc atatccgttc cggtaaccgt atatgttgct gccgaagaag gcgtgacaat
    12421 ttccatggcg gaagttcccc ccgagctcca cgaataagtc gttcctcctg tcgatgtcaa
    12481 tgtatccggc attcccatac acacagttgc catcgaggcg gaagcggaaa tggtcggcag
    12541 c
//
LOCUS       DASTSF010000271         7088 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1621995,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000271 DASTSF010000000
VERSION     DASTSF010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 7088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7088
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1621995"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..919)
                     /locus_tag="VFU15_07850"
     CDS             complement(<1..919)
                     /locus_tag="VFU15_07850"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HEU4717731.1"
                     /translation="MNQAGNSTLPDSTETGRIKILHLKRFWSKCISKKNRLIPGGAFQ
                     EEWLLDHGVLNALGVSLEDSLSFVYNSDAGFDAFENWIIEKNKGSLAEEKINSFNSFY
                     RGEKDGIPASEKILSDADLDFFEKNGYVIIRQAVTPAQCRAAEMKVWEHLGADPDDPS
                     TWYDPHPDRKGIMIQLFHDEALEATRRSEKIRQAYVHLYGHGRLLVTTDRVSFNPPET
                     GSWTFPWPHLHWDVRLDKPVEFGLQGLLYLTDTKAEQGAFRLVPGFQHRINEWLASLP
                     PGRDPQKENLEALGPKPIAANAGDFIIWHH"
     gene            complement(935..3328)
                     /locus_tag="VFU15_07855"
     CDS             complement(935..3328)
                     /locus_tag="VFU15_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014203354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEU4717732.1"
                     /translation="MSEKYLEELNEAQRAAVECTEGPVMIVAGAGSGKTRVLTYRIAH
                     LIRKGIDPFNILSLTFTNKAAREMKERIGKIVSKSEARNLWMGTFHSVFARILRNEAE
                     RLGYPSNFTIYDSDDSKSLIRTILKEQGLDEKVYKPGLVLSRISSAKNNLISSQAYAQ
                     NSNITEEDRMSGKPKLGLIYDLYHRRCFKAGAMDFDDLLFNTNVLLRDYPDLLNKYQD
                     KFRYIMVDEYQDTNFSQYVIVKQLAAKFQNICVVGDDAQSIYAFRGANIQNILNFERD
                     YPEMHTFKLEQNYRSTKNIVNAANAIIKNNRDQLEKNVWTDNHEGDRLQLMKAHTDNE
                     EGQIVARNIFETKMNNQARNRDFAILYRTNAQSRSMEEALRKMNIPYRIYGGLSFYQR
                     KEVKDLLAYYRLAINPHDEEAFRRVINYPARGIGDTSVDKLTIAARDNDVSLWTVCEH
                     IADFGIGINSGTTQRIQDFVTMIQSFRVMLPIENAYDLGNHIANTSGLMRELYTDKTP
                     EGVSRYENVQELLNGLKEFSDGDEAPAIELIENDPELARIEIEQQAKLMAEAGEAEET
                     PRTLDQFMQDIALLTDADKKESEEDMDRVSLMTIHAAKGLEFPYVFIVGLEENLFPSP
                     LSLNSRSDLEEERRLFYVAVTRAEKRATLSYAATRYRWGNLTTCEPSRFIEEIDPKFI
                     DYANSAARNEYSEFNEPSFGLKKTPGGEKEATQALPKKKNLVKASTANSAPALIDNTH
                     LKNLQVGMSVRHERFGTGKVTNLEGNFPNTKATVVFSEAGQKQLLLKFAKLEIINEQ"
     gene            complement(3889..4494)
                     /locus_tag="VFU15_07860"
     CDS             complement(3889..4494)
                     /locus_tag="VFU15_07860"
                     /inference="COORDINATES: protein motif:HMM:NF018446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022841 - potassium ion leak channel
                     activity [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HEU4717733.1"
                     /translation="MSKARLEAFSDGVFAIVITLLILDVHIPRGVSYEELPHALAGMI
                     PNILTYILSFIIVGLYWISHHQILGMMEKINRPIMWMNILGLMTVSFIPFPASLIGEF
                     PMGRLPVVFYGCSLLTANFTGFIAWYYMSRCRQLLRHDVTPQMIRAMNLTFLSVNVAY
                     LVAIGVAFLSVIASYSIFLLVILLLIVRKPWEKHHHSHKSG"
     gene            complement(4511..5737)
                     /locus_tag="VFU15_07865"
     CDS             complement(4511..5737)
                     /locus_tag="VFU15_07865"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU4717734.1"
                     /translation="MKKILVLFCALAVTVAMAAQPAYVFHDKGKALFDQGKYVEAIAQ
                     FDLAIKADASYFEAYLDRGRANMQLGKNDLAMADFNKTVSLNPKYAPGFFWRAKLNAQ
                     EGNDQQAISDYTQAIALNPDFTESYVNRGMLYVKTKQNDPALADFNKAISMDGKNADV
                     FFQRGLIYKSMGKNNEALADFTKVTTLDPKRGDAFFEMGKLNASLGKNDAALANYSKA
                     IGLGITGEEIYKCRAAANVALGKTNDALADYSMLIDQLHTKDGDIYRLRGEVFLSQKN
                     YIAAIKDFNKGLIFKKDDVALLLDRADANYQQGKTKYVQAETDYKKALTLDAANVHAA
                     RGLAQIYFEQEKWQQAVDNLDIVIKNGATGNDYDMRGKCEFRLNNKKSACEDFTKAGQ
                     LGCPDAENDKLNAGCK"
     gene            5810..6148
                     /gene="rbfA"
                     /locus_tag="VFU15_07870"
     CDS             5810..6148
                     /gene="rbfA"
                     /locus_tag="VFU15_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018710163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEU4717735.1"
                     /translation="MESTRQLKFARLIQKEMAEIFRSDSRGLFGNVMITVTQVRMTPD
                     MGIARVYVSLFPVNDKEKLLENIRSHTGELRRSLGNRIRHQVRIIPELVFFIDDSLDY
                     AERIEELLKK"
     gene            6167..6973
                     /locus_tag="VFU15_07875"
     CDS             6167..6973
                     /locus_tag="VFU15_07875"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018476311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU4717736.1"
                     /translation="MEYDPIKRSLGNVFNKSPFLRKVFYRLLDLLLLRAWHVHRELRA
                     WRKGKGNDISILDAGSGYGQHSYYLSGMNPDWKILAVDVKEEQVEDCNRFFAQAGRKN
                     VHFEVADLTVFKKENTYDLAISVDVMEHILEDELVFRNICASLKPGGMLLVSTPSDQG
                     GSDVHGDEESSFIGEHVRDGYNIKDIEDKMKRAGFSKVEALYSYGTPGKISWRLSMKW
                     PIMLLGVSKIFFIIIPFYFLITYPFAYILNWIDTCTKHATGTGLIVKAWK"
BASE COUNT         1542 a         1995 c         1753 g         1798 t
ORIGIN      
        1 catgatgcca gataataaaa tcaccggcat tggcggcgat gggttttggc cccagcgctt
       61 cgaggttttc cttctgcgga tctctaccgg ggggaagcga ggccagccat tcatttatcc
      121 tgtgctgaaa tccgggcacg agcctgaatg cgccttgttc tgccttcgta tccgtgaggt
      181 acagcagtcc ctggagaccg aattccacgg gtttgtcgag gcggacatcc cagtgcaggt
      241 ggggccatgg aaacgtccag ctccctgttt caggcggatt aaagcttaca cgatccgtgg
      301 taaccaaaag tctgccgtgt ccatacagat ggacgtacgc ctgcctgatc ttttccgaac
      361 gtcttgtagc ttccagcgct tcatcgtgaa aaagctggat catgatccct ttgcgatcgg
      421 gatgcggatc ataccaggtg gaaggatcat ccggatcggc cccgagatgc tcccacacct
      481 tcatttctgc cgcgcggcac tgcgccggcg ttacagcctg cctgataatc acgtacccgt
      541 tcttttcaaa aaaatccagg tctgcatcac taagtatttt ttcggaagcg gggatcccgt
      601 ctttttcgcc gcgatagaac gaattgaagc tgtttatctt ttcttccgcc aggctgccct
      661 tattcttttc aatgatccag ttttcgaacg catcaaaacc cgcatcgctg ttgtagacaa
      721 aactgagact gtcttcaagg cttacgccaa gtgcgttgag cacgccatga tcgagcagcc
      781 attcttcctg gaaagcgccg cccgggataa gcctgttctt tttcgaaata cacttgctcc
      841 agaagcgctt caggtggaga atctttatcc gccccgtttc tgtactatca ggaagtgtac
      901 tatttccagc ctggttcatg atcagcagga atcgttattg ttcatttata atttccagct
      961 tggcgaattt cagcaggagc tgtttctgcc cggcttcgct gaaaacgacg gtcgctttgg
     1021 tattcgggaa attgccttcg aggttcgtca ctttacctgt accgaacctt tcgtggcgaa
     1081 cgctcattcc cacctgcagg tttttcaggt gcgtgttgtc gatgagcgcg ggagcggaat
     1141 tggcggtgct cgccttgacg aggtttttct ttttgggaag agcctgcgtc gcttcttttt
     1201 ctccgcccgg cgtttttttc agtccgaacg aaggctcgtt gaactcgctg tattcattcc
     1261 gtgcggcgct gttcgcataa tcgataaact taggatcgat ctcttcgatg aaacggctcg
     1321 gctcgcatgt tgtcagattt ccccagcggt aacgcgtcgc cgcatacgaa agcgtagctc
     1381 gtttttcagc acgtgtgacg gcaacgtaaa acaggcggcg ttcttcttcc aggtctgagc
     1441 gggagttcag cgaaagcggt gaaggaaaca gattctcttc cagcccgaca ataaaaacgt
     1501 agggaaattc gaggcctttc gctgcgtgga tagtcatcag cgaaacacgg tccatgtctt
     1561 cctcgctttc ctttttatcc gcgtcggtca ataacgcgat gtcctgcatg aactggtcga
     1621 gcgtgcgcgg tgtttcttcc gcctcaccgg cttccgccat cagctttgcc tgctgctcga
     1681 tctcgatacg cgccagctcg ggatcattct cgatcagctc gatcgccggc gcttcgtccc
     1741 catcggaaaa ttccttcagt ccgttgagca gttcctgcac gttctcatag cggctcacgc
     1801 cttccggcgt tttgtccgtg tacagctcgc gcatcagccc cgatgtgttc gcaatatgat
     1861 tgccgagatc gtacgcattt tcaatcggca gcatcacgcg gaagctttgg atcatcgtca
     1921 caaaatcctg gatgcgctgc gtggttccgc tgttgatgcc aataccaaaa tcagcaatat
     1981 gctcgcaaac ggtccagagg cttacatcat tgtcccgcgc ggcgatcgtc agcttgtcga
     2041 cgctcgtatc gccgatcccc cgcgccgggt aattgatcac gcgccggaac gcttcttcat
     2101 cgtgcgggtt gatcgcgagg cggtaatacg ccaggaggtc tttcacttcc ttgcgctggt
     2161 aaaaagaaag tccgccgtag atccggtacg gaatattcat tttgcgcagc gcttcttcca
     2221 tcgaacggct ctgcgcattc gtgcggtata gaatggcgaa atcacggttc cgcgcctggt
     2281 tgttcatctt cgtttcgaag atgttccgcg cgacgatctg cccttcctcg ttatcggtgt
     2341 gcgctttcat cagctggagc ctgtcgcctt cgtgattgtc ggtccacaca ttcttttcca
     2401 gctggtcacg gttgttcttg atgatcgcat tcgccgcatt cacgatattt ttcgtgctgc
     2461 ggtaattctg ctccagcttg aacgtgtgca tttcgggata atcacgctcg aagttcagga
     2521 tgttctggat gttcgccccg cggaacgcat agatcgattg cgcatcatcg cccaccacgc
     2581 agatgttctg gaatttcgcc gccagctgct tcacgatcac gtactgcgaa aagttcgtgt
     2641 cctggtactc gtccaccatg atgtagcgga acttatcctg gtatttgttc agcaggtcgg
     2701 gataatcgcg cagcagcacg ttcgtattga aaagcaggtc gtcgaaatcc atggcgcccg
     2761 ccttgaagca gcggcggtgg tagagatcat agatcagtcc cagcttcggt ttcccgctca
     2821 tccggtcctc ttccgtaatg ttcgaattct gcgcgtatgc ctgcgacgag atcaggttgt
     2881 tcttcgccga cgagatccgg ctcagcacga gtcccggctt gtacactttt tcgtccagcc
     2941 cctgctcttt caggatcgtt ctgatcaggc tcttcgaatc gtcgctgtcg taaatggtga
     3001 agttcgacgg gtagcccagc cgttccgcct cattccgcag gatgcgcgca aacacggaat
     3061 ggaacgttcc catccacaaa ttccgcgctt cgcttttcga aacgatcttc ccgatgcgtt
     3121 ccttcatctc ccgcgccgcc ttgttcgtaa atgtcagtga aagaatattg aacggatcga
     3181 tccctttccg gatcaggtgc gcaatgcggt acgtcagcac gcgcgttttt ccggagcccg
     3241 ctcccgccac gatcatcacc ggaccttccg tgcattccac cgctgcccgc tgcgcttcgt
     3301 tcagttcttc aaggtatttc tcgctcactt cgttgaaatg ataaatgatg aattataact
     3361 ggtaaatcgc ttgtataccg cgaatttacg ggtttcggca gcgccaacat tcgctgtgaa
     3421 taatttgctc atttcttgta gcgcatcccc ggctcgtctc atttcctgct ttcccgcttt
     3481 ccgctcgcgg agctcgcttc aatcggggct ggtttggtct ctccgtctcc tttcataacc
     3541 atcctggttt caagttaaaa gttggaaagt tgaaaagtca aaaccacgaa gaacacaaag
     3601 tgttttcaca aaggacacga agaaaaacaa agacaacccg aaacccgaaa cttgaaaccg
     3661 ttttaaccac gaagtacaca aagtgttttc acaaaggaca cgaagaaaaa caaagacaac
     3721 ccgaaacttg aaacttgaaa ccgttttaac cacgaagtac acgaagaaaa acaaagacaa
     3781 cccgaaactt gaaacttgaa accgttttaa ccacgaagta cacaaagatt ttcacaaagt
     3841 gcaggaagga aaacgaaaac aacccgaaac ccggaaaagg aaaaaccctc acccgctctt
     3901 atgcgaatga tgatgcttct cccacggttt ccgcacgatc agcaggagga tcaccagcag
     3961 gaaaatgctg tagctcgcga tcacgctcag gaacgcaacg ccgatcgcga cgaggtaagc
     4021 cacgttcacc gaaagaaagg tcaggttcat ggcgcggatc atttgcggtg taacgtcatg
     4081 acgcagcagt tgccggcagc gcgacatgta ataccatgcg atgaagccgg tgaagttcgc
     4141 cgtcagcagc gagcagccgt aaaacacgac gggcagcctt cccatcggga attctccgat
     4201 cagcgacgcg ggaaacggga tgaagctcac cgtcatcagc ccgaggatgt tcatccacat
     4261 gatcggccgg ttgatctttt ccatcatccc gaggatctgg tgatgcgaga tccagtacaa
     4321 cccgacaatg atgaagctga gaatgtacgt caggatgttc gggatcattc ccgccagcgc
     4381 gtgcggcagc tcttcgtagg atacgcccct gggaatgtgc acgtcgagga tcagcagcgt
     4441 gatcacgatc gcgaaaacgc cgtcgctgaa tgcttccagt cttgctttgc tcatcatcct
     4501 gcgcggaatt ttatttacat cctgcgttca gcttgtcgtt ctctgcgtcc gggcaaccca
     4561 gctggccggc tttggtaaaa tcctcgcacg ccgatttttt attgttgagc ctgaactcgc
     4621 attttccccg catatcgtaa tcattcccgg tcgccccgtt cttgatcacg atatcgagat
     4681 tgtccaccgc ctgctgccat ttttcctgct caaaatagat ctgtgcaagt ccgcgcgcag
     4741 catgcacgtt cgccgcatcc agcgtcagcg cttttttgta atccgtttcc gcctgcacgt
     4801 atttcgtttt tccctgctgg taattcgcgt ctgcacggtc gagcagcagc gccacgtcgt
     4861 cttttttgaa gatcagtcct ttgttgaaat cctttatcgc ggcgatgtaa tttttctgcg
     4921 acaggaaaac ttctccccgc aaacggtaaa tgtccccgtc cttcgtgtgc agctggtcga
     4981 tgagcatgct gtaatccgcc agcgcgtcgt tcgtttttcc cagcgccaca ttcgcggctg
     5041 cgcggcattt gtagatctcc tcgcctgtaa ttcccagccc gatcgctttg gagtaattcg
     5101 ccagcgccgc atcatttttc ccgagcgatg cgttcagctt tcccatttcg aaaaaagcat
     5161 cgccccgctt cgggtcgagc gtcgtgacct tcgtgaaatc ggccagcgct tcattgtttt
     5221 ttcccatgct tttgtaaatg agcccgcgct ggaaaaaaac gtccgcattt ttcccgtcca
     5281 tggaaatcgc cttgttgaaa tccgccagcg cgggatcgtt ctgcttcgtc ttcacgtaca
     5341 gcattccgcg gttcacgtac gattccgtaa aatcgggatt gagcgcaatg gcctgcgtgt
     5401 aatcgctgat cgcctgctgg tcgtttcctt cctgcgcatt cagctttgcc cgccagaaaa
     5461 acccgggcgc gtatttcggg ttcagcgaaa cggtcttgtt gaaatccgcc atggccaggt
     5521 catttttccc gagctgcatg ttcgcacggc cgcggtcgag gtacgcctcg aaatacgacg
     5581 catccgcctt gatcgcgaga tcgaactggg cgatcgcctc cacgtatttt ccctggtcga
     5641 aaagcgcttt ccccttgtcg tggaatacgt acgccggctg cgcggccata gccaccgtca
     5701 cggcaagcgc acagaagaga acgagtatct ttttcattgc cgtgaaggta aacattcacg
     5761 cgaagcctgc cttccgttcc ggcggtgaaa ttccgtacct ttgcccgcta tggaatcgac
     5821 gcgccagctg aaattcgcac gactcatcca gaaagagatg gcggaaattt tccgttcgga
     5881 cagccgcggg cttttcggga acgtaatgat cactgtcaca caggtacgca tgacgcccga
     5941 catgggcatt gcgcgcgtgt acgtgagcct gtttcccgtg aacgacaagg aaaagctgct
     6001 ggaaaatatc cgcagccaca ccggcgagct tcgccgttcg ctcggcaatc gtatccgcca
     6061 ccaggtgcgc atcattcccg aactcgtttt tttcatcgac gattcgctcg attacgcaga
     6121 acgcatcgaa gaacttctca aaaaataatc atcccgcatc ttccgtatgg aatacgatcc
     6181 gatcaaacga agcttaggca acgtattcaa taaatctccg ttcctccgca aggtttttta
     6241 ccggctgctc gatctcctgc tcctgcgtgc atggcacgtg caccgcgagc tgcgtgcgtg
     6301 gcgcaaaggg aaaggcaacg acatttccat cctcgacgcc ggttccggct acgggcagca
     6361 ttcgtattat ttgtcgggaa tgaacccgga ctggaaaata ctcgccgtcg acgtgaagga
     6421 agaacaggtg gaagactgca accgcttctt cgcacaggcg ggacggaaaa acgtgcattt
     6481 cgaagtggcc gatctgactg ttttcaaaaa agaaaacacg tacgaccttg ccatttccgt
     6541 cgacgtgatg gagcacatcc tcgaggacga gctcgttttc cggaacattt gcgcttcgct
     6601 gaagccgggc ggaatgctgc tggtctccac tccttccgac cagggcggat ccgatgttca
     6661 cggtgacgag gaaagctcgt tcatcggcga gcatgtgcgc gacgggtaca acatcaaaga
     6721 catcgaagac aagatgaaac gcgccggctt ctcgaaggtc gaagcgctgt attcctacgg
     6781 aacgccggga aaaatttcat ggcgcctctc gatgaaatgg ccgatcatgc tgctcggcgt
     6841 ttccaaaata tttttcatca ttatcccgtt ttactttctc atcacgtatc cttttgccta
     6901 tatcctgaac tggatcgaca cgtgcacaaa acacgccacc ggcaccggac ttatcgtgaa
     6961 agcgtggaaa tgacccggag ataaatcgtg gatgatgaaa ggaaaccggg tgaagcgcaa
     7021 tcactccgtg caggccgatt cgattctctt tcttactttt cattcatcat ttgtctttcc
     7081 taattcca
//
LOCUS       DASTSF010000272         5256 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_9253,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000272 DASTSF010000000
VERSION     DASTSF010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5256
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9253"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..501
                     /locus_tag="VFU15_07880"
     CDS             <1..501
                     /locus_tag="VFU15_07880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717737.1"
                     /translation="KLREVPGIKIFIIAIVWAIICGFLPATATSTPGIHFFSFRVIAW
                     SLCLSLLIFSLTIPFDIRDHHYDGEKLKTIPSITGIRRSVVIANGSLLFSALIVAVLV
                     FFFHTGSAAMFAAYAAWCIPASVLIAKSSPKRSEYYFSFWVDGLMILLFLLLWAADFI
                     QRFAES"
     gene            complement(454..1755)
                     /gene="hemL"
                     /locus_tag="VFU15_07885"
     CDS             complement(454..1755)
                     /gene="hemL"
                     /locus_tag="VFU15_07885"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008242540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HEU4717738.1"
                     /translation="MNTSNSEKLFSNAKKYFPGGVNSPVRAFRSVGGTPLFIAKGKGS
                     RIWDADGNEFIDYCCSWGPLIHGHSHEDVVNAVKISVENGSSFGAPTESENKLAELIL
                     ENNPFIEKIRFVSSGTEAVMSAIRLARGYTKKNKILKFEGCYHGHSDSLLVKAGSGLV
                     TFGNTSSAGVPEAFVNETIVVPLNNKKAVEDAFAAFGKDIACVIIEPVPANNGLLLQE
                     KSFLAFLRGITQEHRSLLFFDEVISGFRLGFTGAAGHYGIRPDIITYGKIIGGGLPVG
                     AYGASREIMSHISPEGDVYQAGTLSGNPVAMAAGIAQLALCLQKDFYETLKAKCDTLV
                     NAVNKHVKEKKYAVKIFSIGSVFWIAFTGRDLIRSAEEIDPQSMDYFRKFYHLLLEKG
                     IYLGPSGYEVGFVSSAHSDADIRRTVSAFCEALDEVCGPQQ"
     gene            1938..3206
                     /gene="kynU"
                     /locus_tag="VFU15_07890"
     CDS             1938..3206
                     /gene="kynU"
                     /locus_tag="VFU15_07890"
                     /EC_number="3.7.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018476518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030429 - kynureninase activity [Evidence
                     IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kynureninase"
                     /protein_id="HEU4717739.1"
                     /translation="MQQENSIQYARQMDAADPLKKFRERFHFPQFNGEDVLYFTGNSL
                     GLQPKTAREYILQELDDWAKLGVEGHFHGKRPWFSYHEFLTEKTARLVGAKDIEVVVM
                     NQLTVNVHLLMVSFYRPRGLRYKIITEQKAFPSDQYAVASQVKFHGYDPEEAIIEVAP
                     REGEHTIRTEDVLAAIGAHRDELALVFIGGVNYYSGQVFDMETISAAAHKAGAYAGFD
                     LAHAAGNLKLELHKWNVDFACWCSYKYLNSGPGSVAGAFVHEKHADENLVRFAGWWGH
                     DKNTRFRMEKEFIPIPGAEGWQLSNAPVFSMAAHLASLEIFDEAGMDALVEKSKKLSG
                     FLEFVIDEISADNENCLEIITPRDWKQRGCQLSVIAHGYGRDLFNSLMHNGVIPDWRE
                     PNVIRLAPVPLYNSFEDVFKFGERLREAMK"
     gene            complement(3207..4211)
                     /locus_tag="VFU15_07895"
     CDS             complement(3207..4211)
                     /locus_tag="VFU15_07895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717740.1"
                     /translation="MKRIASVIFFTAALLFAFSCKYQPRKITGPGTMIVLKGPDGSDV
                     AQLKNFIPVLTKRLISCGIGQDHFFINDTNGEIDVRFDNTVKLSDTDERYRIMKLFTT
                     SGQLRFHETYTLSELWKNLLNADSIVRSWHANGNRQYPDMPYSLVDLLHPPAPGAYSG
                     TSYDPEHSPVIGFCTVNDTSAVSFVLGMKEVRGQFPENVRFHWTYSHLPENQRLFGLV
                     AIRLKDGDENYFDPGPMKDVVIDENRNGGNRVLNMTFDDVAAASWANMTRSNIGRCVT
                     IEIDNRVLMYPVVQAEIPGGASSISGNFTNAELDFFRGILRSGGLPWPLHVTEEKMVQ
                     "
     gene            complement(4219..>5256)
                     /locus_tag="VFU15_07900"
     CDS             complement(4219..>5256)
                     /locus_tag="VFU15_07900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717741.1"
                     /translation="GCLAAEESLIFDCKEIPVKKISLLIFIFFCVAGFSSCKGKKTGK
                     NDLKIIVHLAGGNSDKTISAIEHRLDDAGISSGDYDVRASGEGIDVSFAEKLLPDLPL
                     LKMTVSGVPELTIRDNYSIFDQLKSLQHLDSMITGDLKDPAATGNTCQCLGAVYLPTG
                     FSRDIVVDNFREQVAIGYAQAKDTAFVDSVLCLPLFRGMLPDDVILLWTAYPSKQVTS
                     FGDFYGLVPAHRSKNLCYTPMKFIKADAQKEKPIPGTPSPGTYHLVLQTDPPGAYEWR
                     KFTQLNNGRSISMEIEGRSYSCPIIRGEISGGIMAMAGIPKEKIDLLKAAFNPEPLPE
                     NLHVTGVTLRK"
BASE COUNT         1216 a         1369 c         1403 g         1268 t
ORIGIN      
        1 aagctgcgcg aggtcccggg aataaaaatt ttcatcatcg cgatcgtgtg ggcgatcatc
       61 tgcggattcc ttccggccac ggccacgtcg acgccgggga tccatttctt ttcgttccgc
      121 gtcatcgcgt ggtcgctgtg cctcagcctg ctcatttttt cactgacgat cccgttcgac
      181 atccgcgatc atcattatga tggggaaaag ctgaagacca taccttccat cacggggatc
      241 cggcgatcgg tcgtcattgc caacggctcg ctgttgtttt cagcgctgat cgtggcggtg
      301 cttgtctttt tctttcatac cggaagcgcc gcgatgttcg ccgcctatgc tgcgtggtgc
      361 attcccgcat ccgtgctcat agcgaaaagc tcgccgaagc ggtcggaata ttatttttcg
      421 ttctgggtgg acggactgat gatcctgctt ttcctattgc tgtgggccgc agacttcatc
      481 cagcgcttcg cagaaagctg ataccgtacg gcggatatct gcatccgaat gcgccgagga
      541 aacaaaaccc acttcatagc cggaaggccc gaggtaaatt cctttttcaa gcaggaggtg
      601 atagaatttc cggaaataat ccatgctctg cggatcgatc tcttccgctg aacggattag
      661 gtccctgccg gtgaacgcga tccagaaaac agagccgatg gagaaaatct ttacggcgta
      721 cttcttttct ttcacgtgct tgttgacggc gttcaccagc gtgtcgcatt tcgctttcaa
      781 tgtttcataa aaatcttttt gaaggcagag tgcaagctgc gcgatgccgg ccgccatggc
      841 gacaggattt cccgaaagcg ttcctgcctg gtaaacgtcg ccttccggtg aaatgtgcga
      901 catgatctca cgcgaagcgc cgtaagcgcc aacgggaagt ccgccgccga ttatttttcc
      961 gtaagtgatg atgtcgggac gaatgccgta atgcccggca gcacccgtga agccgagacg
     1021 gaaaccggaa atgacctcgt caaagaacaa aagcgaccgg tgctcctgtg tgatcccgcg
     1081 cagaaacgcg agaaatgatt tttcctggag gagaagcccg ttgttcgcag gaaccggttc
     1141 gatgatcacg caggcgatgt ctttaccgaa cgcagcgaat gcgtcttcca ccgctttttt
     1201 attgttgagc ggaacgacga tcgtttcgtt cacgaatgct tccggcacgc ctgcggagga
     1261 agtgttgccg aacgtcacga ggcccgaccc ggctttcacg agcagcgaat cgctgtggcc
     1321 gtggtagcaa ccttcgaact tgaggatctt gttttttttc gtgtacccgc gcgcaaggcg
     1381 tatagcgctc atcacggctt cggtcccgga gctgacgaaa cggatctttt caatgaacgg
     1441 gttgttctcc aggatgagct ccgccagttt gttttccgat tcggtgggtg caccgaaaga
     1501 gctcccgttc tccacggaga ttttcaccgc gttcaccaca tcttcatgcg agtgcccgtg
     1561 aatgagcgga ccccaagagc agcaatagtc gatgaattca ttcccgtccg catcccatat
     1621 ccgcgaacct tttcctttgg caatgaacag gggcgttccg ccgacggaac ggaaagcccg
     1681 cacgggagaa ttcacgccgc cggggaaata tttttttgcg tttgaaaaaa gtttttcgga
     1741 attgctggtg ttcataaaaa taaattgaga agcaaaggta agtgatggcg gcggtgaaag
     1801 taaacgaggg aacgacagat tgtgcggatt ggcgcgggtg aagttaacag gagacgatcc
     1861 attcgcgtga acctgcgtca ctcccggccg ttctcccgcg caaagcacga aaactctttc
     1921 tctatctttg gagccttatg caacaggaaa attctattca gtacgcgcgg caaatggatg
     1981 cggccgatcc gctgaaaaaa ttccgggaac gttttcattt tccgcaattc aatggtgagg
     2041 atgtgcttta ttttacgggc aattcgctcg ggcttcagcc gaaaacggcg cgtgaataca
     2101 ttctgcagga gctggacgac tgggcgaagc tcggcgtgga aggacatttt cacggcaagc
     2161 ggccatggtt ttcgtatcat gaattcctga cggaaaaaac agcgcggctg gtcggtgcga
     2221 aagacattga agtggtggtg atgaaccagc tcacggtgaa cgtccacctg ctcatggttt
     2281 cgttttaccg cccgcgcgga ttgcgttaca aaattatcac cgagcagaaa gcgtttccgt
     2341 ccgaccagta cgccgtcgct tcacaggtga aattccacgg ctacgatccg gaagaagcga
     2401 tcattgaagt ggcgccgcgt gaaggcgagc acaccatacg tacggaagat gttctcgcag
     2461 cgatcggggc gcaccgcgac gagctcgcgc tcgtttttat cggcggtgtg aattattatt
     2521 caggccaggt gttcgatatg gaaaccattt ccgcggctgc gcacaaagcc ggcgcatacg
     2581 caggattcga tctcgcacat gcggccggga acctgaagct cgagctgcac aagtggaacg
     2641 tggatttcgc atgctggtgc tcttacaaat acctcaacag cggtccgggc agcgtggccg
     2701 gcgcattcgt gcatgaaaaa cacgcggatg aaaacctcgt acgctttgcc ggatggtggg
     2761 gccacgacaa gaacacgcgc ttcaggatgg aaaaggaatt cattcccatt cccggagcgg
     2821 aaggatggca gctgagcaac gccccggttt tttccatggc ggcgcatctt gcatcgctgg
     2881 aaattttcga cgaggctggt atggatgcgt tggttgaaaa atcgaaaaag ctttccgggt
     2941 ttctcgaatt cgtcattgac gaaatttcgg ccgacaacga aaactgtctc gagatcatca
     3001 ccccgcgcga ctggaaacag cgcggctgcc agctcagcgt cattgcgcac ggctacggcc
     3061 gcgatttgtt caattcactc atgcacaacg gcgtcattcc cgactggcgt gaacccaacg
     3121 tgatccggct cgcgcctgtt ccgctgtaca attccttcga agatgttttc aaattcgggg
     3181 aacgtttgcg tgaagcgatg aagtgatcac tgcaccatct tctcttctgt tacgtgcagc
     3241 ggccagggca atccgccgga acggagtatt ccgcggaaaa aatccagctc tgcattcgtg
     3301 aaattcccgc tgatggacga tgctcctccc ggaatttcgg cctggactac cgggtacatt
     3361 aaaaccctgt tgtcaatttc gatcgtgacg caacggccga tattgctgcg cgtcatgttc
     3421 gcccatgagg cagcggcgac gtcatcaaat gtcatattta atactctgtt tccgccgttc
     3481 ctgttctcat cgatcacgac atctttcatc gggcccggat caaaataatt ctcatcgccg
     3541 tctttcagcc ggatcgcaac cagtccgaac aatctctgat tttccgggag atggctatag
     3601 gtccagtgga aacgcacatt ttccgggaat tgcccgcgca cctctttcat accgaggaca
     3661 aaacttacag ccgacgtatc gttgacggtg caaaaaccaa ttaccgggga atgttcagga
     3721 tcatacgatg tgccggaata agcacccggc gccggtggat gcaggagatc aaccagggaa
     3781 taaggcatat ccgggtactg cctgttcccg ttcgcgtgcc agcttctgac aatgctgtcg
     3841 gcgttcagca gatttttcca gagctcgctt aaggtatatg tctcatggaa acgcagctgt
     3901 ccgctcgtgg tgaacagctt catgatccgg taacgttcat ccgtgtcgct cagcttcact
     3961 gtattatcga agcggacatc tatttccccg ttcgtgtcat tgatgaagaa atggtcctgg
     4021 ccgatgccgc agcttatgag gcgtttcgtc aatacaggaa tgaaattttt aagctgcgcg
     4081 acatcggaac cgtccgggcc tttcagcacg atcattgttc ctggaccggt gatcttacga
     4141 ggctggtatt tgcaggaaaa agcaaaaagc aatgcagcgg taaagaaaat aacggaagcg
     4201 attcttttca tgtgcagatc atttacgaag tgtgacgccc gttacatgca ggttttcggg
     4261 aagcggctcc gggttgaaag ccgctttcag caggtcgatt ttttctttcg gaatcccggc
     4321 catagccata atgcccccgg aaatttctcc gcggatgatg ggacatgaat aggagcggcc
     4381 ctcgatctcc atcgagatgc tgcgcccatt gttgagctgt gtgaattttc gccattcata
     4441 agcgcccggg ggatccgtct gaagtacgag atggtaagtt cccggagacg gtgttcccgg
     4501 aattggtttt tccttctgtg catcagcttt aatgaatttc atcggtgtgt aacatagatt
     4561 tttcgacctg tgcgcaggaa caagtccgta gaaatcaccg aatgaggtca cctgcttcga
     4621 aggataagct gtccacagga gaatcacatc gtccggaagc attccgcgga acaacggaag
     4681 gcacaggacg ctgtcgacaa acgcagtgtc tttcgcctgt gcataaccga tcgcaacctg
     4741 ttcccggaaa ttgtccacga cgatgtccct ggaaaatcct gtcggaagat aaacggcgcc
     4801 gaggcactgg caggtattac ccgtcgcggc cggatctttc agatcgccgg tgatcatact
     4861 gtcgaggtgc tgcagagatt taagctggtc gaagatggaa taattgtcac ggatcgtcaa
     4921 ttcaggaacg ccggagaccg tcattttcag caagggcaga tccggaagaa gtttttctgc
     4981 gaatgaaaca tcgatccctt cgccgctggc ccgcacatcg taatcgcccg aagaaattcc
     5041 cgcgtcatcc aggcggtgtt ctattgctga aattgtcttg tcgctgtttc ccccggcaag
     5101 atgcacgatg attttcaggt cattctttcc cgtctttttc cctttacagg aactgaaacc
     5161 ggcaacacag aaaaaaatga agatgagcag ggaaattttt ttcacgggta tttctttgca
     5221 atcaaaaata agcgattctt ccgcggcaag acaacc
//
LOCUS       DASTSF010000273         6283 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_980343,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000273 DASTSF010000000
VERSION     DASTSF010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 6283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6283
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_980343"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..412)
                     /locus_tag="VFU15_07905"
     CDS             complement(<1..412)
                     /locus_tag="VFU15_07905"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU4717742.1"
                     /translation="MKRVDIMFPVYYGNLHEIQPCVDAVIPAFTRSLAEYDWHIIFAV
                     NGKNPGEVITLVKKIHETHPNVWYDYAERPGKGSGIIHSWSASPADIIAYMDVDLSTD
                     IGNFPDLVKGVENGYDLCIGSRYHPGSDVQRSLKR"
     gene            complement(409..1278)
                     /locus_tag="VFU15_07910"
     CDS             complement(409..1278)
                     /locus_tag="VFU15_07910"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEU4717743.1"
                     /translation="MKILVTGANGFLGTHVLRALEKKHEVTGLVRRETRPGESRMVVF
                     RDTDDPVIENEISRAEVVLHIASQLHGPYREMYAANVSFTKKLVDLALKHGCARFIYI
                     SSENVTQGNSDIYSTTKMLAEKEAERFPGALILRPTVIYGPGDEKYVGRLAGIIAKYP
                     FVPVLGDGKAKFQFIYVEDIVHILENAIEKHITGTWTIAGPESISYDQFMQVLMEAMK
                     KKKRLLHVPLWLLRPLSHAFNLIFRDPPLTPSQIDNLSKDRAYDISGILKQFSYSPTY
                     LRTGLQKMLENIA"
     gene            complement(1357..2118)
                     /locus_tag="VFU15_07915"
     CDS             complement(1357..2118)
                     /locus_tag="VFU15_07915"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU4717744.1"
                     /translation="MEKSYYEEYYRYERSHWWFTVRSRILENTLRRHLPKGKKPRILN
                     IGVATGASTEMLMKLGEVTSVEYDKDCCEFVSGILGTKVYNESITALPFGDSSFDIVC
                     AFDVIEHVADHVTAVAEMERVCSPGGLVFVTVPAFMSLWSKHDVINHHERRYRLKGLR
                     ELFRPRKGHEVRSSYFNTILFPPIFIFRQAGRLLFRRRSTEEATSDFETVSTTSFTGK
                     IINRMLGALFSMEIFLLRFMRFPAGVSILFLWQKD"
     gene            2204..3490
                     /locus_tag="VFU15_07920"
     CDS             2204..3490
                     /locus_tag="VFU15_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004462125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4910 domain-containing protein"
                     /protein_id="HEU4717745.1"
                     /translation="MNEKDIRHLMESYFDRLWPICRSITGNGLRESLKILSEIVPLQL
                     TEVPTGTEVFDWTIPKEWNITDAYIITPDGKKICDFKVNNLHVVNYSIPVEKEMTFDE
                     LDRHLFSIPQMPSAIPYVTSYYKETWGFCLAHSEREKLPANGLYKVKIASSLENGSLT
                     YGEHVLEGKTKEEILFSTYVCHPSMANNELSGPLVAAFLYTKLAALPERRFTYRFVFA
                     PETIGVIAFLQKNGAALKKNLRAGYVITCAGDAGAFTYKRSKDPLSAADHVAEHVLKH
                     SGKKFTAIDFAIGGSDERQYCSPGFNFPVGSLMRTMYQRYPEYHTSLDNKSFISFAAM
                     EETVEMYLRFAKLHEMNRKYTNTVPFCEPQLGKRGLYPDVGAQKNRAEELSRRLHLLS
                     WADGNNDLVAIAEKNGTCALDYENTIRELAAAGLLV"
     gene            3459..4925
                     /locus_tag="VFU15_07925"
     CDS             3459..4925
                     /locus_tag="VFU15_07925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717746.1"
                     /translation="MSLLRQDSWSERTTMAHDERSFTEFKKDFYGKFPDLSVFECGDV
                     NLLKVVLDGLKVNYAERFPARTPVFSSLLFHRAFLFLKRKRLRGTGEAVKELRAAGGK
                     NAWCVDFEGKSSPGENGQLVSFYFSRLMRILGSSAALLNGSSRKTYSGGIAFGDVLAE
                     GMVESLSADDKVFRDHLRRTFTRIRETGKLNATELLSVKNAFQRFFDSARGWNRIFQT
                     AKPECVFFVMHYHREGFVYAMKKNGVRSVELQHGLIAKEDIFYVFPPQVRSIRDRALF
                     ADRIFVYGEYWKEVLAEGAEYGEEHVRVLGYYADEGAPADTKLPDAVLKFIDGKKTAL
                     VCTQTFMHDEYDLLVRRLSALALEKGWNCVFLVKPHPHEQLADYAPLDNLPNVKTVQV
                     PLPLLMQSADVHFSVYSTTHFDAARFGVKSIALPSEKYGDYVRGITASGVAELYDFES
                     DPFSGGEKSRMSEKQKTFFYAPFDVSSFPAERLIAMTK"
     gene            4974..5900
                     /locus_tag="VFU15_07930"
     CDS             4974..5900
                     /locus_tag="VFU15_07930"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HEU4717747.1"
                     /translation="MRVSVIIPCFNAGEFIGRCLASVQAQTYPDLEIICVDDGSSDDT
                     AEKIKAIAGNSEKKIKLILRANAGASSARNTGLAAASGEYIQFLDADDTIGPGKIAAQ
                     VEEAVKNDYPDIIVSPYVRISAGGTQDINCEKDPWIGVMRSRLGCTCSNLWKKSAVLA
                     AGKWNTQLKSSQEYDLIFRMLKNRGTVFHVMNNETEVHVREGSISTSNPGENWKRFLA
                     LRSDVITFLKENQPREFSAHREVYLRLLFDAIRMAYPYQQDAAEKVFSAQIPRDFVPS
                     PGAVNTRSYIFLFRLFGFRTAERIRKIFRRSA"
     gene            5897..>6283
                     /locus_tag="VFU15_07935"
     CDS             5897..>6283
                     /locus_tag="VFU15_07935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU4717748.1"
                     /translation="MKILYYSPHPALNLSAQTGYGTHMREMIRAFREAGHEVKTCIRG
                     GEQMPVSSGGATAGNGKRLLKKIIPRFLWRTLKERRLMREDKISEAVLAKAMEEFQPD
                     VIYERAAYLCISGRNVAVRKKIPHIVE"
BASE COUNT         1501 a         1624 c         1732 g         1426 t
ORIGIN      
        1 tgcgtttgag cgagcgctga acatccgagc ccgggtgata acgggagccg atgcagagat
       61 cataaccatt ttcgacgccc ttcaccaggt cggggaaatt accgatatcg gttgaaaggt
      121 cgacatccat gtacgcgatg atgtccgccg ggctcgccga ccaggagtga atgatcccgc
      181 tgccttttcc cgggcgttcg gcgtaatcgt accacacgtt gggatgcgtt tcgtggatct
      241 tcttcacgag cgtgatcact tccccgggat tctttccgtt caccgcaaaa ataatgtgcc
      301 agtcgtattc cgccagcgaa cgggtgaacg cgggaatgac ggcatccacg cagggctgta
      361 tttcgtggag atttccgtaa tacacgggaa acatgatatc aacccttttc atgcgatgtt
      421 ctccagcatt ttttgcagac ctgtgcgtaa ataagtgggg gagtagctga actgctttaa
      481 aatcccgctt atgtcatacg ctctgtcttt ggaaaggttg tcgatctgcg aaggtgtaag
      541 cgggggatcc cggaatatga ggttaaatgc atgagaaagc gggcgaagca gccacagcgg
      601 aacatgaagg agccgtttct ttttcttcat cgcttccatc agcacctgca tgaactgatc
      661 atagctgatg ctttcgggac ctgcgatcgt ccaggtgccg gtgatgtgtt tttctatcgc
      721 attttcgagt atgtgcacga tatcttccac gtaaatgaac tggaatttcg ctttcccgtc
      781 gccgagaacg ggtacgaacg ggtatttcgc gatgatcccg gcgagacgcc cgacatattt
      841 ttcatccccc ggtccgtaaa tgaccgtggg gcgcaggatg agcgcgccgg gaaacctttc
      901 cgcttctttt tcggccagca ttttcgtcgt ggaataaatg tcactgtttc cctgcgtgac
      961 attttcggag ctgatgtaaa tgaaccgggc gcatccgtgc ttcagcgcca ggtcgacgag
     1021 ctttttggtg aaagatacat tcgccgcata catttccctg tacggaccgt gaagctgcga
     1081 tgcgatgtgg agaacgacct ccgcgcgtga aatctcgttt tcgatcacag ggtcgtctgt
     1141 atcacggaaa acgaccatgc ggctttcgcc cggacgggtt tcgcggcgga caaggccggt
     1201 cacctcgtgt tttttttcca gcgcccgcag cacatgcgta cccaggaatc cgttcgcgcc
     1261 ggtgacgagt attttcataa catcgcgtcg attttctgaa accggcctgc gtgtactacg
     1321 gctgagaatg aatggcagga gacgatcggg gtaaaattaa tctttttgcc agagaaagag
     1381 aatcgataca ccggccggga aacgcatgaa gcggagaaga aatatttcca tggaaaaaag
     1441 cgcgccgagc atccggttga tgatcttccc ggtgaatgaa gtagtgctta cggtttcaaa
     1501 atccgacgtg gcttcttcgg tgctgcgcct gcggaaaagg aggcggcctg cctggcggaa
     1561 aatgaaaatg ggcggaaaaa ggatcgtgtt gaaatagctc gacctgacct catgtccttt
     1621 tcgcgggcgg aaaagttcgc gtaatccttt cagccggtac ctgcgctcgt ggtggttgat
     1681 cacatcgtgc ttgctccaga ggctcatgaa cgcaggaacg gtgacgaaaa ccagtccgcc
     1741 cggcgagcat acgcgttcca tttccgcaac agccgtcacg tggtcagcta catgctcgat
     1801 cacgtcgaat gcgcagacga tgtcgaagga agaatccccg aaaggcagcg cggttatgga
     1861 ttcgttgtaa acttttgtgc cgagaatgcc ggaaacgaat tcgcagcagt ccttgtcata
     1921 ttcaaccgaa gtcacctcgc cgagcttcat cagcatttca gtgctggcgc ccgtggcgac
     1981 gccgatattc agtatgcgcg gttttttccc tttgggcagg tgcctgcgaa gcgtgttttc
     2041 aagaatgcgg ctcctgacgg taaaccacca gtgagacctt tcgtaccggt aatattcttc
     2101 gtaataggat ttttccatga cggagatcca aaggtaaatc ttatccggct cgcaggccgt
     2161 cgctgcgcat ccccgttttg tccttatgtg tatctttaaa aaaatgaacg aaaaggatat
     2221 ccggcatctc atggaaagct atttcgaccg gctctggccc atctgccgga gcatcacggg
     2281 aaacgggctg agggaatcgc tgaaaatatt gtctgagatc gttccgctgc agctgacgga
     2341 agttccgacc ggcacggaag tgttcgactg gacgatcccg aaggaatgga acataaccga
     2401 cgcgtacatc attacaccgg acgggaaaaa aatatgcgat ttcaaagtga acaacctgca
     2461 cgttgtgaac tacagcatcc cggtcgaaaa agagatgaca ttcgatgagc ttgaccggca
     2521 cctgttctcc atcccgcaaa tgccgtccgc catcccgtac gtgacgagct actataagga
     2581 aacttggggc ttctgtctgg cgcacagcga acgggagaag ctgccggcaa acggtcttta
     2641 caaagtgaag atcgcctctt cgctggaaaa tggctcgctg acgtatggcg aacatgtcct
     2701 cgaaggaaaa acaaaagagg aaattctttt ctccacctat gtgtgccatc cgtccatggc
     2761 gaacaacgag ctttccggtc cgctcgtggc ggcttttctg tacacgaagc tggctgcgct
     2821 cccggaacgg agatttacct accggttcgt gttcgccccg gaaaccatcg gcgtgatcgc
     2881 gttcctgcaa aagaacggcg cggcgctgaa aaaaaatctc cgcgcgggtt atgtgatcac
     2941 gtgcgcaggt gatgccggtg cgttcacgta taaaagatca aaggatccgc tttcggcggc
     3001 agatcatgtc gcagagcatg tgctgaaaca ttccgggaaa aaattcaccg cgatcgattt
     3061 cgcgatcggc ggaagcgacg agcgacagta ttgttcaccc gggttcaact tccccgtagg
     3121 ttcgctcatg cggacgatgt accagcgtta cccggaatac cacacctcgc tcgacaataa
     3181 gtcgttcatt tcgttcgccg cgatggagga aacggtggaa atgtacctgc gtttcgcgaa
     3241 gctgcacgag atgaaccgga aatacacgaa tacggttcct ttctgcgaac cgcaacttgg
     3301 aaagcgcgga ctttatcccg acgtaggcgc gcagaagaac agggcggaag agctttcccg
     3361 ccgtcttcat ctcctgtcat gggccgacgg gaacaatgat ctcgtcgcga tcgcagagaa
     3421 gaacggcacc tgcgccctcg attatgaaaa tacgatccgt gagcttgctg cggcaggact
     3481 cctggtctga acggacgact atggcgcacg acgagcgatc gtttaccgaa ttcaaaaaag
     3541 atttttacgg gaagttcccg gacctttccg ttttcgaatg cggcgacgtc aacctgctga
     3601 aagtcgttct cgacggactc aaagtaaatt atgcggagcg cttccccgca cggacaccgg
     3661 tcttttcctc gttgcttttt catcgtgcat ttcttttcct caaaaggaaa agattacgcg
     3721 ggaccggcga ggcggtaaaa gagctccggg ccgccggcgg caaaaacgcc tggtgcgtgg
     3781 atttcgaagg aaaaagttct cccggtgaga acgggcagct tgtttctttc tatttcagcc
     3841 ggctgatgcg catcctcggc agcagcgccg ctttgctcaa cggatcgtcg cggaaaacat
     3901 acagcggcgg cattgctttc ggcgacgtgc tcgctgaagg gatggtggaa tcgttgtctg
     3961 cggatgacaa ggtcttccgg gaccatttgc ggcgcacgtt cacacgcata cgcgagaccg
     4021 ggaagctgaa tgcaacggaa ttactcagcg tgaaaaatgc gttccagcgt tttttcgaca
     4081 gcgcgcgcgg gtggaaccgc atcttccaaa cggcgaaacc ggaatgcgtt ttttttgtca
     4141 tgcactacca ccgcgaagga tttgtttacg ccatgaaaaa aaacggggta cgcagcgtcg
     4201 aactgcagca cgggctgatc gcgaaagaag atattttcta cgtattccct ccgcaggtac
     4261 gcagcatacg cgaccgggcg cttttcgccg accgtatttt cgtttacggg gaatactgga
     4321 aggaagtgct ggcggaagga gccgaatacg gcgaagaaca tgttcgcgtg ctcggctatt
     4381 acgccgatga gggcgcgccc gcggacacaa agctgcccga tgccgttttg aaatttatcg
     4441 atggaaaaaa aacagcgctg gtgtgcacgc agacattcat gcacgacgag tacgacctgc
     4501 ttgtgcggcg gctttccgcg ctcgcgctgg aaaaaggatg gaactgtgtt ttcctcgtga
     4561 agccgcaccc gcatgagcag cttgccgatt atgcaccgct ggacaacctg cccaatgtaa
     4621 aaaccgtgca ggtgccgctt cccctcctca tgcagtcggc tgacgttcat ttttccgttt
     4681 attcgaccac tcatttcgat gctgcccgtt ttggcgtaaa aagtatcgcg ctgccttcgg
     4741 agaaatacgg agattatgtt cgcgggatca cggcatcggg tgtggcggag ctgtacgatt
     4801 ttgaaagcga cccgttcagc ggcggagaaa aaagccggat gagcgaaaag caaaaaacgt
     4861 ttttttacgc gccgtttgac gtctcgtctt tcccggcgga acgactgatc gcaatgacga
     4921 aataaaatcg tgcccatgtt cctgtatatt cacatgaaag tctgagatca gctatgcggg
     4981 tgtccgtcat tattccctgt ttcaatgccg gtgaattcat cgggcgttgc cttgcatccg
     5041 tgcaggcgca aacgtatccc gacctggaga tcatctgcgt cgatgatggt tcgtcggatg
     5101 atacggcgga aaagatcaaa gcgatcgccg gcaacagcga aaaaaagata aagctgatcc
     5161 tccgggccaa tgcaggtgca tcgtcggcac gcaataccgg gcttgccgct gcttccggcg
     5221 aatacatcca gttcctcgac gcagacgata cgatcggtcc gggcaagatc gcagcgcagg
     5281 tggaagaggc cgtgaagaac gattatcccg atatcatcgt gtcgccatac gtcaggatct
     5341 cagccggcgg aacgcaggat atcaactgcg aaaaagatcc gtggatcggt gttatgcgtt
     5401 cgaggctggg ctgtacttgt tccaatctct ggaaaaaaag cgcggtgctt gctgcaggaa
     5461 aatggaacac gcagctgaaa agcagccagg aatacgatct tattttccgc atgctgaaaa
     5521 acaggggaac cgtatttcac gtgatgaaca atgaaacgga agtgcatgtg cgcgaaggat
     5581 cgatctccac atcaaatccc ggtgagaact ggaagcgttt cctggcattg cggagcgatg
     5641 tcatcacttt cctgaaagaa aaccagccac gtgaattttc cgcccaccgc gaagtttacc
     5701 tccggcttct tttcgatgcg atacgcatgg catatcctta ccagcaggat gcggcggaaa
     5761 aagtattttc agcgcagatc ccccgcgatt ttgttccgtc gcccggagcg gtaaacacaa
     5821 ggagctatat ttttctgttc cggcttttcg gattccggac ggccgaacgc atccggaaaa
     5881 ttttcaggag aagcgcatga agatcctgta ttattccccg catcccgccc tgaacctgtc
     5941 ggcacagacc ggttacggaa cgcacatgcg tgaaatgatc cgtgctttcc gggaagccgg
     6001 ccatgaagtg aaaacctgca tccgcggggg cgagcagatg cctgtttcat ccggcggagc
     6061 aacggccgga aacgggaaac gcctgctgaa aaagatcatt ccccggtttt tgtggaggac
     6121 actcaaggaa cgcaggctga tgcgggagga taaaatttcc gaagctgttc tcgcaaaagc
     6181 catggaggaa tttcaacccg acgtgatcta cgaacgtgcg gcgtacctct gcatttcggg
     6241 aaggaacgtt gctgtccgca aaaaaatacc gcacatcgtg gaa
//
LOCUS       DASTSF010000274         4623 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1664468,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000274 DASTSF010000000
VERSION     DASTSF010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4623
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1664468"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..472
                     /locus_tag="VFU15_07940"
     CDS             <1..472
                     /locus_tag="VFU15_07940"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HEU4717749.1"
                     /translation="LWEEKKYNWWVYLVSVFAVYGTLIVALIFVALILVGFGFSRDSS
                     KMQEVTEILRANKGLMVFTALTAGVMEELTFRGYILSRFELLFRNSGLAIFLSSVLFG
                     LMHFGYGNMFQLAGPFVIGFIFALYYWKFRNIKVVIICHFLWDMMSLAVSTMKH"
     gene            complement(518..1678)
                     /gene="purT"
                     /locus_tag="VFU15_07945"
     CDS             complement(518..1678)
                     /gene="purT"
                     /locus_tag="VFU15_07945"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010932738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HEU4717750.1"
                     /translation="MKKKILLLGSGELGKEFTIAAKRLGQYVIAVDSYNDAPAQQVAD
                     EREVISMLDGAALDALVAKHRPDIIVPEIEAIRTERFYEYEKQNIQVVPSARAANYTM
                     NRKAIRDLAAKELGLRTAKYAYAKTPEEFRAAVDAIGIPCVVKPLMSSSGKGQSVVKT
                     ESDIEKSWNYAMTGTRGDLMEVIVEEFIRFESEITLLTVTQKNAPTVFCPPIGHRQER
                     GDYQESWMPHPMSEAHIKEAQDMAEKVTRALSGYGIWGVEFFLAAEGVYFSELSPRPH
                     DTGMVTLANTLNLNEFELHLRAVLGLPIPETALQRAGASAVILASQEGVPVVSGLNEA
                     LNEPDVDVKIFGKPVMRPYRRMGVALAWDKPGTDVMRLVEKAKAAAAKVKMQ"
     gene            1804..3732
                     /locus_tag="VFU15_07950"
     CDS             1804..3732
                     /locus_tag="VFU15_07950"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU4717751.1"
                     /translation="MQEDIAGKTAELEEALSIVVSEEERLPLLNEISWLLRQANITKS
                     RAYAQEAFTIAEKTGNGRQRALALRALGSAEDYFSNPQQALIYLDEAASFFRAVNDDA
                     NVIACNNLIGKTFLYLSDFPRALEVFQENQRLAQKEGKPQLIADALLTQGVVLLRSGD
                     FRKAKTLLEKSKAISHESDYIAGEGRALLNLSALYVDLKQYEEGLRCALEGVSIMEKR
                     RDMVDVASCISNAGSCCIGLGRYEEAETYFLRSREMRKANNEVTHLVNNSISMGELYT
                     QMKRYGEAEKNLEEALSLCIDSGNNFQIMHTYEAFAGLCEAQGRYEDALKYYKLFHAT
                     ERLLTNEESQKKMRNLEVRAEVERLENEKELYRKHASVLEEANRQISEQKDLIEEKNK
                     DITDSIHYARRIQEALLPSEEEMKRLLPESFVFYRPKDIVSGDFYWVTEKDGRIFFAV
                     ADCTGHGVPGALMSMIGHNLLHRSINENGLSGPGEILSRLDHDLREMLKQNSADSGGN
                     DGMDVVLIVKERGKNLIRFSSSRRPLWIFREGEMTEFKGERIYVGGKGEGFRSFRTNE
                     FSCAAGDRLYLFSDGFPDQFGGPEMKKYQTARFRKFLQEIAVLPAHEQKELIEKEFFQ
                     WKGSNEQQDDILVAGIVV"
     gene            3810..4157
                     /locus_tag="VFU15_07955"
     CDS             3810..4157
                     /locus_tag="VFU15_07955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717752.1"
                     /translation="MKRFLFLFSILLLVSCDKKTQGGQAGKNNFSHTVTAGSVPGKND
                     TVAGAAEEAAASPDSLVEDDYRDFVVIDNGPGAEPDVLFFGWDGNESLLRNTADGWKI
                     VASFRVPGWRCPC"
     gene            complement(4159..>4623)
                     /locus_tag="VFU15_07960"
     CDS             complement(4159..>4623)
                     /locus_tag="VFU15_07960"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU4717753.1"
                     /translation="NRISVGLSDNMRDLVWALHPEHATLDQLVSRLREFSGDLSEEID
                     IRFRRDFPASVPQQKIPKEVLRNTYLVFKEALHNAVKYSGAEEIVIAVTINRAGISIE
                     ISDNGKGFDAQAEEGKGNGLANMKHRVHSVNGKISIVSSPGKGARIAFSVPV"
BASE COUNT         1018 a         1259 c         1296 g         1050 t
ORIGIN      
        1 gctgtgggaa gaaaagaaat acaactggtg ggtgtacctc gtttccgtct tcgccgttta
       61 cggcacgctg atcgtcgcgc tgatctttgt tgcactgatt ctcgtcgggt tcggcttcag
      121 ccgcgacagt tcgaagatgc aggaggtgac ggaaatcctc agggcgaaca aaggactgat
      181 ggtgttcacc gcgcttaccg ccggcgtgat ggaagagctg acgttccgcg ggtatattct
      241 ttcgcgtttc gagctgctgt tccggaattc cggtcttgcg atcttccttt cgtccgtgct
      301 gttcggactg atgcatttcg gttacgggaa tatgttccag ctggccggac cgttcgtgat
      361 cgggttcatt ttcgcgttgt attactggaa attccgcaac ataaaagttg tgatcatctg
      421 tcactttctc tgggacatga tgtcgctcgc ggtttcaacg atgaaacact gaagctgcat
      481 tttgcgcacc cggtattccc gggcggatac cgaacggtca ctgcattttc actttcgcgg
      541 cggcggcttt cgctttttcc acgagccgca tcacgtccgt gccgggcttg tcccacgcga
      601 gcgcgacgcc catcctgcgg tacgggcgca tcacgggttt cccgaaaatt ttcacgtcca
      661 cgtccggctc gttcagcgct tcgttcagtc cggaaacaac aggcacgcct tcctgcgaag
      721 cgagaatgac cgcgctggcc cccgcacgct gcaaagccgt ttcaggaatg ggaagtccga
      781 gcacggcgcg caggtgcagc tcgaattcat tcaggtttaa cgtattcgcg agcgtcacca
      841 tgccggtatc atgcggtcgc ggggaaagct ccgaaaaata aacgccttca gccgcgagga
      901 aaaattccac gccccagatg ccgtagccgg aaagcgcgcg cgtcactttt tccgccatat
      961 cctgcgcttc tttgatgtgc gcttcgctca tcgggtgcgg catccagctt tcctggtaat
     1021 cgccgcgctc ctgccggtga ccgatcggcg gacaaaaaac agtcggcgca tttttctgcg
     1081 taacagtgag cagcgtgatc tccgattcga accggatgaa ttcttccacg atcacttcca
     1141 tcaggtcgcc gcgcgttccc gtcatcgcat agttccatga tttttcaatg tcgctttccg
     1201 tcttcacgac gctctgtcct ttcccggacg aagacatcag cggcttcacc acgcacggaa
     1261 tcccgatcgc gtcgacggcg gcgcggaatt cttccggtgt ttttgcgtac gcatattttg
     1321 ccgtgcggag acccagctct ttcgccgcga gatcacggat cgccttgcgg ttcatcgtgt
     1381 aattcgccgc acgtgcgctc ggcaccacct ggatattctg tttttcgtat tcgtaaaaac
     1441 gttccgtgcg tatcgcttcg atctcgggaa cgataatatc gggtctgtgc ttcgccacca
     1501 gtgcatcgag cgcagcgcca tcgagcatgc tgatcacttc gcgctcgtcg gccacctgct
     1561 gggcaggcgc atcgttgtac gaatcgacgg caatcacgta ttgcccgaga cgtttggccg
     1621 caatcgtgaa ttctttgccg agctcgcccg aaccgagaag caggattttc tttttcatac
     1681 ggataatttg gaagcgcaag ataggaagac gacgtgaacc cgggcgggta accagtccgg
     1741 ggttctgaaa ccaaaaccca aaatccggaa cccggaattc gttgtttagt atcttagcga
     1801 tctatgcagg aggatatcgc cggaaagacc gcggagctgg aagaagcact gagcattgtt
     1861 gtttcggaag aagagcgtct gcccctgctc aatgaaattt cgtggctgct gcgccaggcg
     1921 aacatcacca aaagccgcgc gtatgcacag gaagcgttca ccattgcgga gaaaaccggc
     1981 aacggacgtc agcgtgcact ggcgcttcgt gcgctcggca gcgccgaaga ttatttcagc
     2041 aacccgcagc aggccctgat ctatctcgac gaagcagctt catttttccg cgcggtgaat
     2101 gatgatgcca acgtgatcgc gtgcaacaat ctcatcggga aaacatttct ttacctcagt
     2161 gattttccgc gggcgctcga ggttttccag gagaaccagc ggcttgcgca aaaggaagga
     2221 aaaccgcagc tcatcgcgga tgcattgctg acgcagggcg tcgtgctttt gcgaagcggc
     2281 gatttccgga aagcgaaaac attgctggaa aagagcaagg cgatcagcca tgaaagcgat
     2341 tacatcgccg gggaaggacg ggcgctgctg aatctcagcg cgctttatgt cgacctcaaa
     2401 caatatgagg aagggctgcg ctgtgcgctg gaaggcgttt ccattatgga aaaacgcagg
     2461 gacatggtcg acgtcgccag ctgcatcagc aacgcaggtt cttgctgcat cgggctcggg
     2521 cgttacgagg aggcggaaac atattttctg cgcagccggg agatgcgcaa agccaataac
     2581 gaggttacgc acctggtgaa caattccatt tccatgggcg agctgtacac gcagatgaaa
     2641 cggtacggcg aggcggaaaa aaacctggag gaggcgcttt ccctgtgcat cgattcgggg
     2701 aacaatttcc agatcatgca tacatacgaa gcgttcgccg gcctctgcga agcgcaggga
     2761 cgctatgagg atgcgctgaa gtattacaag ctcttccatg caacggaacg gctcctgacc
     2821 aacgaggaaa gccagaagaa aatgcgcaac ctggaggtga gggcggaagt ggaaaggctc
     2881 gagaatgaaa aagagctcta ccggaagcac gcctccgtgc tcgaagaagc caaccggcag
     2941 atcagcgagc agaaagacct tatcgaagaa aagaacaagg atatcaccga cagcattcac
     3001 tatgcccgcc gcatacagga agcgctgctt ccttcggaag aagagatgaa acgcctcctg
     3061 ccggaaagct tcgtgttcta ccggccaaag gatattgtca gcggcgattt ctactgggtg
     3121 acggaaaagg acggcaggat atttttcgct gtcgccgatt gcacgggcca cggtgttccc
     3181 ggtgcgctca tgagcatgat cggccacaac cttctccacc gcagcatcaa cgagaacggc
     3241 ctctccggtc ccggggagat cctttcccgc ctcgatcacg acctgcgcga aatgctcaag
     3301 cagaacagtg ccgattccgg cggcaacgac ggcatggatg tcgtgctcat cgtgaaagaa
     3361 cgcggaaaga atctcatccg cttcagcagc tcccggcgcc ctttatggat cttccgcgag
     3421 ggagaaatga cggaattcaa aggcgagcgc atttatgttg gcggaaaggg agaaggtttc
     3481 cggagcttcc gtacgaatga attttcctgc gccgccgggg atcgtcttta tcttttttcc
     3541 gatggttttc ccgaccagtt cggcggaccc gaaatgaaaa aataccagac ggcacgcttc
     3601 cggaaattcc tccaggaaat tgccgtcctg cctgcgcacg aacagaagga actgatcgaa
     3661 aaggaatttt tccagtggaa agggagcaat gagcagcagg atgatattct cgttgcgggc
     3721 atagtggtgt gaacggacat ttgctcaatt cctgccacgc ttgtccgttt tctttcccgg
     3781 gtttgctctt cctgttttag tttcgtttca tgaagcgttt cctgtttctt ttcagcattc
     3841 ttcttcttgt ttcctgcgat aaaaaaacgc agggcggaca ggccgggaaa aataatttca
     3901 gccatactgt cactgccggt tcggtgcccg gaaaaaacga tacggttgcc ggtgctgcag
     3961 aagaagcggc tgcttccccg gattcacttg tcgaagatga ctaccgcgac tttgtcgtga
     4021 tcgataacgg tcctggtgcg gagcccgacg tgctcttttt cggctgggat ggaaatgaat
     4081 ctcttctgcg caacacagcg gacggatgga agatcgtcgc ttcgttccgg gtgcccggct
     4141 ggcgctgccc ctgctgattc acacaggaac ggaaaacgcg atccgcgcgc ctttcccggg
     4201 cgatgagaca atgctgatct tcccgttcac cgaatgaacg cgatgtttca tgttggcaag
     4261 cccgttccct tttccttctt cagcttgcgc gtcgaaccct ttcccgttgt ccgagatttc
     4321 gatggaaatg cccgcacggt tgatcgtaac cgcgattact atttcttccg cgccggaata
     4381 tttgacggcg ttgtgcagcg cttccttgaa aacgaggtac gtgttgcgca gcacttcctt
     4441 cgggattttc tgctgtggaa cagacgccgg gaaatcccgt cggaaacgga tgtcgatctc
     4501 ttcggaaaga tcgccggaaa attcccgcag gcgcgacacg agctggtcga gcgtggcgtg
     4561 ctccgggtga agggcccaga cgagatcacg catgttgtcg gaaagcccga cggaaatgcg
     4621 gtt
//
LOCUS       DASTSF010000275        14542 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1116190,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000275 DASTSF010000000
VERSION     DASTSF010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 14542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14542
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1116190"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(48..1478)
                     /locus_tag="VFU15_07965"
     CDS             complement(48..1478)
                     /locus_tag="VFU15_07965"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF013079.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HEU4717754.1"
                     /translation="MTVLKRAKSLPAETMLVIIAGKKSSMNRFRLSESAIAEINKAWE
                     SDRKQVQVSNGKQQVIVCLKDNSAEAHTRKEMLRRSAAQAVTAINEQKIQHITLADAD
                     DNTGEILAFAEGMLLSNYQFLRYKVKNPAKDANSLTQVDVFSARLKDQSLDRLKIVMQ
                     ATFMARDLVNEPVSTLDAVKLAKEFQEMGKDAGFRVEVFGKSKIESLKMGGLLAVNRG
                     SDIPPTFTVMEYKPRNAKNKKPVVLVGKGVVFDTGGYNIKVGAGMETMKCDMGGAAAV
                     GASMWAIAKAKLPVHVIALVPATDNRINGSAYVAGDVLTMYSGLTVEVLNTDAEGRLI
                     LADALAYGEQFDPQLVLEFSTLTGAAMAAIGSYGIVAMGTASERVKQKLKTSGENVYE
                     RLAEFPFWPEYDELIKSDVAEIKNLGGPYGGAITAGKFLAQFVKSPFMHFDIAGPAFL
                     SAKDHYRPKGGTGVGVRLLFDYFASI"
     gene            1556..2680
                     /gene="mqnC"
                     /locus_tag="VFU15_07970"
     CDS             1556..2680
                     /gene="mqnC"
                     /locus_tag="VFU15_07970"
                     /EC_number="1.21.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008241420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic dehypoxanthinyl futalosine synthase"
                     /protein_id="HEU4717755.1"
                     /translation="MKTDELLKRATELDFLSAEEGVFLFENAPTAELMFAANQVRQKK
                     KPGKKVTWIIDRNLNTTNVCIANCKFCNFYRIPGHAESYITDMETYRRKITETIRYGG
                     EQLLLQGGHHPDLGLSYYTDLFRGLKKMFPNIKLHALGPPEIAHITKLEKSTHAEVLS
                     ALIEAGLDSLPGAGAEILNDRVRRLISKGKCSGSEWLDVMRAAHKLGLTTSATMMFGH
                     IETIAERFEHLVWIRQVQSERPSGAKGFIAFIPWPFQDDGTLLRRVKGVKNNVSGDEY
                     IRMLALSRLMLPNIDNIQASWLTVGKEVAQMCLHGGANDFGSIMIEENVVSAAGAPHR
                     FTYKSIQNAIREAGFEPQLRNQQYEDRAIPENIEEQVINY"
     gene            2790..3266
                     /locus_tag="VFU15_07975"
     CDS             2790..3266
                     /locus_tag="VFU15_07975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717756.1"
                     /translation="MNFTRGFLALGFIGVLYFGCKPQPVFPVEPALTFKEYQQKEGGD
                     TLKVVFSFTDGDGDIGVLPTGSDTNMVLTAYYKTPAGNYHVWLNPQQTDSIYYSYRIP
                     KLTASQKGLEGDIYLTISTREVIAAYHDTLEFNAFLLDQSGHKSNLVRTPELILLP"
     gene            3266..5995
                     /locus_tag="VFU15_07980"
     CDS             3266..5995
                     /locus_tag="VFU15_07980"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HEU4717757.1"
                     /translation="MSRRTTLALSFLLGVFGSLKATHNIAGQITAEVVHDTVTGNCSN
                     YLYYKVTVTTYTNSLSPADRCDLTIDWGDNTSSTVHRSNGITGSCPNATMGVDLSSQG
                     FANTKENVYVGFHAFPGAGTYHISVKDPNRVAGILNIPNSVNVPFYLETTITIDPLFG
                     CNSTPYLTTIPLDKACTGHCYYHNPGAVDPDGDSLSYSIGPCLDTTGLPIAGYSLPNV
                     IGGGSLAIDPVTGDLSWCSPGVAGKYNLVIYITEWKRLPNGNHVKIGTVLRDMSIDVQ
                     NNCNNNNPDISNLQDLCVDAGTLVNFNLTVDDPDGDSVKLQGYGSPFSSSPAASVVPS
                     ATYTAVPYNANFNWQTTCNNVRVQPWTATFKATDNHSPVALTDIESVNITVVSPGPAF
                     LTTTPQGSQMNLSWGQNPCDPLVNPCIGYKVYRRQGPTSWMHAQCETGVPAYTGFVQI
                     GTVTGVNNTTFVDDNNGSGLVPGVDYCYRVCAFFLDGAESYSSPESCNELRRDVPVIV
                     NVDIMSTGLSDSVYVRWVNALANGIDFDTTQHVGPYILELERSAGYSLSSPATIATFT
                     VNSFYQLPTVFIDTLRQTSQTPYTYRLKFYGSGGSDYIGNSQPASSTYAGAMPSDNTV
                     TLTWQDSVPWNNFEYAIYRLNNVTGQWDSLTTVASHTYADDSLENGIDYCYYVKAHGS
                     YFNPTLPPIQYNRSQRVCATPVDLTPPCPPQLAVNSDCSTAVNQLTWTNPINMNCGTD
                     DVVLYHIYYAAVEGDPFELISTVTVPADTTITYSNLASVAGCYAVTAVDTFGNESAYS
                     DIVCVDNCPVYMLPNVFTPNGDNVNDFFIPFPYQYVKDIDLKIYDRWGNLIFETTDPD
                     INWDGRDMKSHKLCTDGVYYYTCTVNEIRLQGIVPRQLTGFVHLFGKDVGQFH"
     gene            6012..6593
                     /locus_tag="VFU15_07985"
     CDS             6012..6593
                     /locus_tag="VFU15_07985"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016198613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEU4717758.1"
                     /translation="MFTGIVEATGRIEKIRNEKGNVHFSLSCPFLAELKIDQSVAHNG
                     VCLTVAGIDETSYIVTAVTETLRKTNLGVLKEGDEVNLERCMRVGDRLDGHIVQGHVD
                     TTGVCEEVRDDNGSTIFTFSYEKEKGITVEKGSVCVNGVSLTVIDSAPGKFSVTIIPY
                     TFSHTTFRNLGKGDKVNLEFDIVGKYIAALASR"
     gene            6649..8298
                     /locus_tag="VFU15_07990"
     CDS             6649..8298
                     /locus_tag="VFU15_07990"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEU4717759.1"
                     /translation="MKNFGGRRRNFSGDSKSGKRSGGKKPSGKRTFGKTAEGGSKKYS
                     GERGSNPFGDRKKSFGDRDDSRPRRKFDDSKPSRPFGDRKRSFGDRDDSRPRRKFDDS
                     KPSRPFGDRKKSFGDRDDSRPRRKFDDSKPSRPFGDRKKSFGDRDDSRPRRKFDDSKS
                     SRPFGDRKKSFGDRDDSRPRRKFDDSKPSRPFGDRKKSFGDREDSRPRRKFDDSKPSR
                     PFGDRKKSFGDRDDSRPRRKFNDEKPHKEFIERKKPSEEKGNDEWQVFDNKDEANDFR
                     EESQGRPKKSRQRPPRKFAPTPAGENEVIRLNRFIANTGLCSRREADELIEAGVIKVN
                     GKIVTTLGTKIGPADTVHYGDQLLKKEMLVYILLNKPKDYITTTEDPQERKTVMHLVE
                     GATPERVYPVGRLDRNTTGLLLLTNDGDLAERLMHPKYGIRKIYHVTLDKNLRKEDFE
                     AFENGVELEDGFIKADELNFTEDGSNKKEVGIAIHSGRNRIVRRLFEHLGYSVISLDR
                     VVYAGLNKKNLQRGKWRSLTPQEVGFLKMQVGGEKKKSFRK"
     gene            8422..8775
                     /gene="panD"
                     /locus_tag="VFU15_07995"
     CDS             8422..8775
                     /gene="panD"
                     /locus_tag="VFU15_07995"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HEU4717760.1"
                     /translation="MMIQVMKSKIHRARVTEANLDYIGSITIDENLMDAANLVENEQV
                     HVLNYRNGERIITYVIKGGRGSGVICLNGPAALKFSPGDDVIVISYAGMSTEEAKTFT
                     PVVIFPDTATNQPKS"
     gene            8779..9786
                     /locus_tag="VFU15_08000"
     CDS             8779..9786
                     /locus_tag="VFU15_08000"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEU4717761.1"
                     /translation="MKSNLRQAFQFALFFGLGIGLTYWQFHSLSPEQQDQFFIALRKA
                     NYGWMVVAILIGALAHLSRAMRWQLLLSPLGRKAGLGSRWYAVMIGYLANYGIPRSGE
                     VIRCTTLATNEDVPFSESFGTVIVERLVDTLCLALVFLIVLAIEFTQLSDLWTHFIWG
                     PASLKFGKMMQNKTLVIALGVFILLFIAAFIFLRRRIAEKIKGKLGNFIKGFVGGLVA
                     IKKVPKPGLFIFHSLFIWAGYLFSLYACFFCFPETAGLSVNTALVLLLFGTFGVIFTP
                     GGIGAYQLIVTAILIHLFASAEPAAAPFSWLSWGAQVMTVVLFTGITFAAKNSLNRLS
                     Q"
     gene            9783..10301
                     /locus_tag="VFU15_08005"
     CDS             9783..10301
                     /locus_tag="VFU15_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HEU4717762.1"
                     /translation="MTTQEFLRKKIHTKESIHHLLERLRYFGKKIVFTNGCFDILHLG
                     HVDYLAKAKDFGDFLVVGLNSDASVKILDKGSNRPLQDEASRSNVIASLHFTDAVILF
                     DEETPYELIRFVKPGVLVKGADYDADETDPKSKKYIVGSDLVRANGGEIKTIELVQGY
                     STSAIEKKIRNS"
     gene            complement(10702..11433)
                     /locus_tag="VFU15_08010"
     CDS             complement(10702..11433)
                     /locus_tag="VFU15_08010"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013750554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEU4717763.1"
                     /translation="MYNTLQPENILFLDIETVPAYASYDLLPEQWKKLWDLKAALLKK
                     SEDDTPETLYNRAGIYAEFGKIICISVGFLKHAGGAEQFRIKSFAGDDEKKLLGEFAE
                     LLRRHFNRPDHRLCAHNGKEFDFPWIARRMVVQGIQLPALLDTAGKKPWETTHLDTME
                     MWKFGDFKSHTSLNLLANLFGIPTPKDDIDGSMVHVVYYRDKDLARIVKYCQKDVLTV
                     VQLLRKYRGEELLADETVIMSDWEK"
     gene            complement(11603..12004)
                     /locus_tag="VFU15_08015"
     CDS             complement(11603..12004)
                     /locus_tag="VFU15_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015808007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717764.1"
                     /translation="MKKDIEIPVVEDVAIAVILEDDNGENVWNVYIVNMKDVPIRNVL
                     VTSKGYGEMDGAKRSTSTLRHFFTEIPANDFAKIEPIMDFTFGLSNEYWVSFYIGEQI
                     YDKRYIFLPESISEKNFTKVPLINIEGVMIR"
     gene            complement(12066..13247)
                     /locus_tag="VFU15_08020"
     CDS             complement(12066..13247)
                     /locus_tag="VFU15_08020"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEU4717765.1"
                     /translation="MKKLRKFLKRLGLFILFLLVAVNLFIVFTGRFYLYKGVWNTYLR
                     GRKGPSATEYKIFDNREVKNGTPQPWALGKDYNQKEIPGKILDSLMQIETGAFLVIKD
                     DSIRCEKYWGEFSNASYTNSFSMAKTITSILVGCAIKDGAIKSVDDPVCNYLPEFKQP
                     QFEKITLKNLLEMSSGIAFDEDYVNPLAYPAEAYYGSDLRTLTFGYKKMNGTPGDNFL
                     YLSGNTQLLGFVVEKATGKHLADYASEKLWIPMGCEHSAFWSLDHKDGDEKAFCCFNS
                     NARDFARLGKLYLDSGRWNGNQLVPESYALASVQPTGMHDFGGRPCLTYGYNWWMIPE
                     YAGHHIFYARGILGQYIIVVPDLKMIIVRLGQKRLPNDSNDLPLDVHYYLNAALQMYG
                     S"
     gene            13441..13971
                     /locus_tag="VFU15_08025"
     CDS             13441..13971
                     /locus_tag="VFU15_08025"
                     /inference="COORDINATES: protein motif:HMM:NF040205.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5819 family protein"
                     /protein_id="HEU4717766.1"
                     /translation="MNRKRILFLGGALIFLLHFFFTAAYSFPGLAGNRFLQRVTGHYM
                     FPLFNQDWKIFAPDPPVFSKKLFYRAAFSDGTSSGWTDPGAELLEKHYRNRFGNCRDR
                     FAIYEAMGREMEAGEDSLPLKYVLNDCASRFGAKKISIIECCRVYVSVPEFKTGEGGG
                     MLTLDRYFKKDLRENH"
     gene            13958..>14542
                     /locus_tag="VFU15_08030"
     CDS             13958..>14542
                     /locus_tag="VFU15_08030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717767.1"
                     /translation="MKTIDRLSERFTSSVVQAGTIALFRKCLYAFLFFRTITLLPAAN
                     EFWGPGSYLPFYYRQSSFLLKLLNPLQSEAFHAYYLFFAIAQLALIAISFFVKKRQRL
                     LCAGIYFFTAALHHKMAAIENGGEQLAMILLFFLLLMNENGTGMLNSTVTNFAFLAAR
                     VQLVFVYFVAGITKLKGVHWIDGTALHYVFATDEY"
BASE COUNT         3476 a         3893 c         3810 g         3363 t
ORIGIN      
        1 tccgtgcaac aggcaccggc ggtcatttcc ctgcgcgcag ttcattttca gatcgaagcg
       61 aagtaatcga aaagcaggcg aacgccgacg ccggttccgc ctttgggacg gtaatgatct
      121 ttcgcggaaa gaaacgccgg acctgcgatg tcgaaatgca tgaacggaga tttcacaaac
      181 tgtgccagga actttccggc ggtaattgcg ccgccgtaag gtccgccgag attttttatt
      241 tcagcgacat ccgatttgat gagctcatcg tattccggcc agaacggaaa ttccgccagg
      301 cgttcataga cgttttcacc tgatgttttg agcttttgct tcacgcgttc ggaagccgtt
      361 cccatcgcca caattccgta ggaaccaatg gcagccatgg ctgcgcccgt caacgttgaa
      421 aattcgagaa ccagctgcgg atcgaattgc tcaccgtaag cgagcgcatc ggcgagaata
      481 agcctgcctt ccgcgtcagt gttgagcacc tcgacggtca gcccgctgta catcgtgagc
      541 acatcgccgg ccacgtacgc gcttccgttg atgcggttgt ccgtagcggg aacgagcgcg
      601 atcacgtgca cgggaagttt agctttggcg atggcccaca tggaagcccc gactgcggcg
      661 gccccgccca tgtcgcattt cattgtttcc atgccggcgc cgaccttgat gttatagccg
      721 cccgtatcga acaccacgcc tttcccgaca agcacgacag gttttttgtt tttcgcattg
      781 cgcggcttgt attccatcac ggtgaacgtg ggcggaatgt cactgccgcg attgaccgcc
      841 agcagtccgc ccattttcag ggattcgatt ttcgatttgc cgaaaacctc cacacggaaa
      901 ccggcgtctt ttcccatttc ctggaattcc ttcgcaagct ttacggcatc gagcgtgctg
      961 acgggctcat tgacaagatc gcgtgccatg aaagtggcct gcatcacgat cttcagcctg
     1021 tcgagcgact ggtctttaag tcttgcagaa aaaacatcga cctgtgtgag cgagttggcg
     1081 tccttcgccg gatttttcac cttatagcga aggaactggt aattggaaag cagcattcct
     1141 tcggcaaaag cgaggatctc gcctgtattg tcatctgcat cggccagtgt aatgtgctga
     1201 attttctgtt cgttgatcgc tgtaaccgcc tgtgcggccg accggcgtaa catttccttc
     1261 ctggtgtgcg cttccgcgga attatccttg aggcagacga tcacctgctg ttttccattc
     1321 gacacctgca cctgcttacg gtcggattcc caggctttgt ttatctctgc gatcgcgctt
     1381 tccgaaaggc gaaaccggtt catggaagat ttttttccgg caatgatcac cagcatggtt
     1441 tcagcaggaa gcgatttagc acgtttgaga acggtcatgt tttgtaattt tgagggcact
     1501 aaaagtaaca aaacgggttg aagtgccaac cgccttttcc atgagcgagc agggaatgaa
     1561 aacagacgaa ttactgaaga gagcgacgga gctcgatttt ctttcagccg aagaaggcgt
     1621 ttttcttttt gaaaatgcgc cgacggccga gctcatgttt gccgcgaacc aggtgagaca
     1681 aaagaagaag ccagggaaaa aagtcacgtg gatcatcgac cgcaacctca atacaacgaa
     1741 cgtttgcatt gccaactgca agttctgcaa tttctaccgc atcccgggac atgccgaatc
     1801 gtacatcacc gatatggaaa cataccggcg caagatcacg gaaacgatcc gttacggcgg
     1861 cgaacaattg ctgctgcagg gcggccatca tcccgacctg ggactgagct attatacaga
     1921 tctgttccgc ggcctgaaaa aaatgttccc gaacatcaag ctgcatgcat tgggtccgcc
     1981 ggagatcgca cacatcacaa agctggagaa aagcacacat gctgaagtcc tttccgcgct
     2041 gatcgaagcc gggctcgatt cgcttcccgg cgcaggtgcg gaaatcctca acgaccgcgt
     2101 ccggcggctg atctcgaaag ggaaatgttc aggcagcgaa tggctggacg taatgcgcgc
     2161 tgcgcataag cttggtctta ccacctccgc aaccatgatg ttcggccaca tcgaaacgat
     2221 cgccgaacga ttcgagcacc tggtatggat acggcaggtg cagagcgagc ggccgtctgg
     2281 tgcgaaaggt ttcatcgctt tcattccgtg gcctttccag gacgacggga cgcttttgcg
     2341 acgcgtgaaa ggcgtgaaaa acaacgtttc gggcgatgaa tacatccgga tgctggcgct
     2401 gagccggctg atgctgccga acatcgataa catacaggct tcctggctta cagttggaaa
     2461 agaggtggcg caaatgtgcc tgcacggggg cgcgaacgat ttcggttcga tcatgatcga
     2521 ggagaatgtc gtttctgcag ccggggcgcc gcaccgtttc acctacaaaa gcatccagaa
     2581 tgcaatacgc gaagcgggtt tcgagcccca gctgaggaac cagcaatatg aagaccgcgc
     2641 gatcccggaa aacattgagg agcaggtgat caattactga ggctgcacca attaccggtc
     2701 aatatccgcc acctgtaaaa ccttcggttt atccttgcct gaaagggcat attgaagtat
     2761 ctttgaagtt accctccatt atacaggaaa tgaatttcac gcgtggattt ctcgcacttg
     2821 gcttcatcgg cgtcctttat ttcggatgca agccccagcc tgtattcccg gttgagcctg
     2881 cgctgacgtt caaagagtac cagcagaagg aaggcggaga cacgctgaaa gttgtgttca
     2941 gcttcacgga cggcgatggc gacatcggcg ttttacctac aggttcggat acaaacatgg
     3001 tgctcacggc gtattacaaa actcccgccg gaaattatca cgtctggctt aatccgcagc
     3061 aaaccgattc gatctactat tcttaccgca tacccaagct gacggcttcg cagaaagggc
     3121 tggaaggaga tatttacctg acgatctcca cccgcgaggt cattgccgct tatcatgaca
     3181 cgctcgagtt caatgctttc ctgctcgacc agtcgggaca caaaagcaac ctcgtgcgca
     3241 cacctgaatt aattctttta ccgtgatgtc aagacgcact actctcgctc tgtctttcct
     3301 gctcggcgtt ttcggttcgc tgaaagcgac gcacaacatt gcaggacaga tcacggcgga
     3361 agttgtgcat gatacggtaa ccggtaactg cagcaattat ctctattaca aagtaacggt
     3421 cactacctac acgaattccc tgagcccggc cgaccggtgc gatctgacga tcgactgggg
     3481 cgacaatacg agcagcactg tccatcgctc gaacgggatt accggcagct gtcccaatgc
     3541 gacaatgggc gttgatcttt cctcccaggg ctttgcgaac accaaggaaa atgtttatgt
     3601 cgggttccat gcctttccgg gggcgggaac gtaccacatc agcgtgaaag acccgaaccg
     3661 cgtagcaggc atcctgaaca ttccgaactc ggtgaacgtg ccgttctatc ttgaaacgac
     3721 aatcacgatc gacccgcttt tcgggtgcaa ttccacgccg tatctcacaa ccatcccgct
     3781 tgacaaagcc tgcacgggac attgctatta ccacaatccc ggcgcggtcg acccggacgg
     3841 tgacagtctt tcctacagca tcggtccgtg cctcgacacg accggtcttc ccattgcggg
     3901 atattcattg cccaacgtga tcggcggcgg aagcctggcg atcgatccgg ttacgggtga
     3961 tctttcctgg tgctcgccgg gcgttgcggg aaaatacaat ctcgttattt acatcacaga
     4021 atggaagcgc ctgccgaacg gaaatcacgt caagatcggg acggttctcc gcgatatgtc
     4081 catcgacgtg cagaacaatt gcaacaacaa caacccggat atttccaacc tgcaggatct
     4141 ctgcgtcgat gcaggtacgc tcgtgaattt caacctcacg gtagatgacc cggacggaga
     4201 cagcgtgaaa ttgcagggct acggaagccc gttcagctca tcacctgccg ccagcgtcgt
     4261 tccttccgcc acctatacgg ctgttcccta caacgcaaat ttcaactggc agaccacctg
     4321 caacaatgtg cgcgtacagc cgtggacagc gactttcaag gcaacggaca accattcgcc
     4381 ggttgcactt accgacattg aatcggtgaa catcacggtg gtttctcccg gtcctgcttt
     4441 tcttaccact acgccgcagg gctcacagat gaatctttcc tggggacaaa atccctgcga
     4501 tccgctggtg aacccgtgca tcggttataa agtttaccgc cggcagggac cgacatcgtg
     4561 gatgcacgct caatgtgaaa cgggcgttcc ggcgtatacc gggttcgttc agatcggaac
     4621 cgtaaccggg gttaataata caacgttcgt ggacgataac aacggcagcg gactcgtgcc
     4681 gggcgtggat tactgctacc gtgtttgcgc atttttcctc gacggcgcgg aaagctattc
     4741 ttcaccggaa tcatgcaacg agctgagaag agatgtgccg gtgatcgtga acgtcgacat
     4801 aatgtctacc ggcctgagcg attctgtata cgtacgctgg gtgaatgcgc tggcgaacgg
     4861 aatcgatttc gatacgacgc agcacgtcgg tccctacatt ctcgaactcg aacgttcggc
     4921 gggatattct ctttcttccc cggctacgat cgctacgttc acagtgaatt ccttctacca
     4981 gctgccgacg gtattcatcg atacgctgcg ccagaccagc cagacaccgt acacctaccg
     5041 cctgaaattt tatggttccg gcggcagtga ttacatcggg aattcccagc ctgcatcttc
     5101 cacttacgcc ggggcgatgc cttccgacaa tacggttaca ctgacctggc aggacagcgt
     5161 cccgtggaac aatttcgagt atgcgatcta ccgcctgaac aatgttaccg gtcagtggga
     5221 cagcctcacc acggttgcct cacacaccta tgccgacgac agtctcgaga acggcattga
     5281 ttactgctat tatgtaaaag cgcacggttc ctatttcaac ccgacactgc cgcctatcca
     5341 gtacaaccgt tcccagcgcg tatgcgcgac acccgtcgac cttactcctc catgtccgcc
     5401 gcagcttgcc gtgaattcag actgcagcac cgccgtgaac caactgacgt ggaccaaccc
     5461 catcaacatg aattgcggaa cggatgatgt cgtgctttac catatttatt acgccgccgt
     5521 ggaaggcgat ccattcgaat taataagcac ggttactgtt ccggcggata caacgatcac
     5581 gtattcgaat ctcgcatcag tggcaggctg ctatgctgtc accgcggtcg acacattcgg
     5641 gaatgaaagc gcctacagcg atatcgtgtg cgtcgacaac tgccccgtct acatgcttcc
     5701 gaacgtattc acccctaacg gtgacaacgt aaatgatttc ttcattccgt tcccgtacca
     5761 gtacgtaaaa gacatcgacc tgaagatcta cgaccgctgg ggaaatctca ttttcgaaac
     5821 aacagatccg gatatcaact gggacggacg cgatatgaaa tcgcacaagc tctgcacgga
     5881 cggtgtgtat tattacacct gcacggtcaa cgagatccgc ctgcagggaa ttgttccgcg
     5941 acagcttacg ggctttgtcc atctcttcgg aaaagacgta ggccagttcc attaagatct
     6001 tcttcaccgc tatgtttaca ggaatcgtgg aagcgacagg ccgcatcgaa aagatccgga
     6061 acgagaaagg caatgtgcat ttttcgcttt cctgtccttt tctcgcagaa ctgaaaatcg
     6121 accagagcgt ggcgcacaac ggcgtctgcc tcaccgtagc aggcattgac gaaaccagct
     6181 atatagtaac cgcggttacg gaaacgctgc gcaagaccaa tctaggtgtg ctgaaggaag
     6241 gagatgaagt gaacctcgag cgctgcatgc gcgtgggcga ccgcctggac gggcatatcg
     6301 tgcagggaca cgtggacacg accggcgtct gcgaggaagt gcgcgacgac aacggcagca
     6361 cgatcttcac tttcagctac gaaaaagaaa aaggcattac cgtcgaaaaa ggttccgtct
     6421 gcgtcaatgg cgtgagcctg acagtgatcg attccgcacc cgggaaattt tccgttacca
     6481 tcattccgta tacgttttca cacacgacat tccggaacct gggcaaaggc gacaaggtca
     6541 acctcgagtt cgacatcgtc gggaaataca tcgctgcact cgcttcccgc tgaaaccgcg
     6601 ggattgacgt cgtttattca cctacctttg cagctcaata caaccatcat gaaaaacttt
     6661 ggcggccggc gcaggaactt ttccggtgac agcaaatccg ggaaaagatc cggcggaaaa
     6721 aaaccatccg gcaaaaggac gttcggcaaa accgctgaag gcggaagcaa aaaatacagc
     6781 ggggaaagag gaagcaaccc gttcggcgac cgtaaaaaat cattcggcga ccgtgatgat
     6841 tcacgcccgc gaaggaaatt tgacgacagc aaaccttcgc gccctttcgg cgaccgtaaa
     6901 agatcattcg gcgaccgtga tgattcacgt ccgcgccgga aattcgacga cagcaaacct
     6961 tcgcgccctt tcggcgaccg caaaaaatca ttcggcgatc gtgatgattc acgcccacgc
     7021 cggaaattcg acgacagcaa accttcgcgc ccttttggcg accgcaaaaa atcattcggc
     7081 gaccgcgacg attcacgccc gcgaagaaaa ttcgacgaca gcaaatcttc gcgccctttc
     7141 ggcgaccgca aaaaatcatt cggcgaccgt gatgattcac gcccgcgaag aaaattcgac
     7201 gacagcaaac cttcgcgccc tttcggcgat cgtaaaaaat cattcggcga ccgcgaggat
     7261 tcacgcccgc gaagaaaatt cgacgacagc aaaccttcac ggccgttcgg cgaccgtaaa
     7321 aaatcattcg gcgatcgcga cgattcacgt ccgcgacgga aattcaacga cgagaagccg
     7381 cataaagagt tcatcgaacg caaaaaacca tcagaagaaa aaggaaacga cgagtggcag
     7441 gtattcgaca ataaagatga agcgaacgat ttccgtgaag agagccaggg ccgcccgaaa
     7501 aaatcacggc agcgcccgcc gcgaaaattt gcaccgaccc cggcgggaga gaacgaagtg
     7561 atccgtctca accgtttcat agcgaataca ggactttgct cgcgcaggga agcggacgaa
     7621 ctcatcgaag cgggtgtgat caaagtgaat ggaaagatcg ttacgacgct gggcacgaag
     7681 atcggtccgg ccgacacggt gcattacggc gaccagctgc tgaaaaaaga aatgctcgtg
     7741 tatatcctgc tgaacaagcc gaaagattac atcacgacga cagaagatcc gcaggaacgc
     7801 aaaaccgtga tgcacctggt ggagggcgcc acgcccgagc gcgtttaccc ggtgggccgc
     7861 ctcgaccgca ataccacggg actgctgctg ctgacgaacg acggcgatct tgccgaacgc
     7921 ctgatgcacc cgaagtacgg catccggaaa atttatcacg tgacgctcga caaaaacctg
     7981 cgtaaagaag atttcgaagc gttcgaaaac ggcgtggagc tggaagacgg cttcatcaaa
     8041 gcggacgagc tgaattttac cgaagacggt tccaacaaaa aagaagtcgg catcgcgatc
     8101 cattccggca ggaaccgcat cgtgcggagg ctgttcgagc atctcggcta ttccgttatt
     8161 tcgctcgacc gcgtcgtgta tgccggactg aacaagaaaa atctccagcg ggggaaatgg
     8221 agaagtctta caccgcagga agtaggattc ctgaagatgc aggtgggcgg agaaaagaaa
     8281 aagagtttcc ggaaataaat tttcaccttt ccgggcacag accacgttac tttcatcacg
     8341 gttccgctgt ggaaatgcag cggcgcaggg tttccggggg ccacttccgt atcgggaatc
     8401 cttgctaact ttgacgccgc tatgatgatc caagtgatga aatcgaaaat tcaccgcgcc
     8461 cgggtgacgg aagcaaacct cgattatatc ggaagcatta cgatcgacga gaacctgatg
     8521 gacgcggcga acctggtcga aaacgagcag gtgcatgtac tgaattaccg caacggggaa
     8581 cgcattatca cttatgtgat aaaaggcgga agaggatcgg gcgtgatctg cctgaacggt
     8641 cctgcggcgc tgaaattttc acccggcgat gacgtgatcg tcatttcata cgccggcatg
     8701 agcacagaag aagcgaaaac gttcactccc gtcgttattt ttcccgacac cgccacgaac
     8761 caaccaaaat cctgacgttt gaagtcaaac ctccggcagg cattccagtt tgcgctgttt
     8821 ttcgggctcg gcatcgggct aacgtactgg cagtttcaca gcctttcacc ggagcagcag
     8881 gaccagtttt tcattgcgtt gcgcaaggcg aattacggct ggatggttgt cgccatcctt
     8941 atcggcgcac tggcgcacct gagccgggcg atgcgctggc agctgctgct ttcgccgctc
     9001 ggccgtaaag cgggactggg cagccgctgg tacgcggtga tgatcggcta cctcgccaat
     9061 tatggtattc cgcgttcggg tgaagtgatc cgctgcacca cgcttgcgac gaatgaagac
     9121 gttccgtttt ccgaatcgtt cgggacggtg atcgtggagc gtctcgtcga cacactttgc
     9181 ctcgcgctgg tgttccttat tgtgctggcg atcgagttca cgcagctgag cgacctgtgg
     9241 acacatttca tctggggacc ggcatcgctg aaattcggaa agatgatgca gaacaaaacg
     9301 ctggtcatcg cgctgggcgt ttttatactg ctgttcattg ccgctttcat cttcctgaga
     9361 cggagaattg cggagaagat caaagggaag ctgggaaatt tcatcaaagg attcgtcggc
     9421 ggactggttg cgataaaaaa agttccgaag ccgggtcttt ttattttcca ctcgctgttc
     9481 atctgggcgg gttatctttt ttcgctgtat gcgtgctttt tctgttttcc tgaaacggcc
     9541 ggactttccg tgaacacggc gctcgtgctc ctgctgttcg ggacgttcgg ggtgatcttc
     9601 acacccggcg gcatcggcgc ttaccagctg atcgtaaccg cgatcctcat ccatcttttc
     9661 gcttccgcag aaccggcggc ggcgcctttc tcctggctga gctggggcgc gcaggtaatg
     9721 acggtggtgc ttttcacagg aatcacgttc gcggcaaaaa attcacttaa ccggctttcc
     9781 caatgacgac gcaggaattt ctccggaaaa aaatccacac gaaggaatcc attcatcacc
     9841 tgctcgaacg cctgcgttat ttcgggaaga agatcgtgtt cacgaacggc tgcttcgata
     9901 ttcttcacct cggtcacgtg gattacctgg cgaaagcaaa agacttcggc gattttctcg
     9961 tggtcggact gaacagcgac gcctcggtaa aaatactgga caagggaagc aaccgcccgc
    10021 tgcaggatga agcttcgcgc agcaacgtga tcgcctcgct tcattttacg gatgcggtta
    10081 tccttttcga tgaagaaacg ccgtatgagc tgatccgttt cgtgaaaccg ggcgtgctgg
    10141 taaaaggcgc ggattacgat gcggacgaaa ccgatccgaa aagcaaaaaa tatatcgtgg
    10201 ggtctgatct tgtgcgggcc aatggcgggg aaataaaaac gatcgagctc gtgcagggtt
    10261 attcgacgag cgcgatcgag aagaagatca ggaattcgta agagtttggg gaagtttcgg
    10321 gttttgaaat actcttcaat tgaaaattat ttcctgtttc acttcaggtg acgcaagttt
    10381 ttcttcgcgc ttccgggaga attctttttt taacacggag atcacaaagg ttgttgcaca
    10441 gaggaccacg gagaacagca aaccgaaaat cattgcctgt ttcacttcaa atgacgcaag
    10501 ttttccttcg cgtttccgga agaagttttt ttaacacgga gtttcacaga ggttgttgca
    10561 cagaggacca cagagaacag caaaccgaaa atcattgcct cctgtttcaa ttcaggtgac
    10621 gcaaattttt cttcgcgccc ccatgagaat tcctttctga caggtgcacc aggtttgcga
    10681 aatattgaac cggtaaccgg ctcacttttc ccagtcgctc atgatcacgg tttcatccgc
    10741 cagcaattcc tcgccgcggt atttgcgcag cagctgcacg accgtaagca catccttctg
    10801 gcaatacttc acaatacgcg caagatcctt gtcgcggtaa tacacgacgt gcaccatgct
    10861 tccgtcaatg tcatccttgg gtgtgggaat gccgaaaagg ttcgcgagca ggttgagcga
    10921 agtgtggctt ttgaaatcgc cgaacttcca catttccatc gtgtcgaggt gcgtggtttc
    10981 ccatggtttc ttccccgccg tatcgagcag cgcggggagc tggatgccct gcacgaccat
    11041 gcggcgcgcg atccagggga aatcgaactc tttgccattg tgcgcacaga ggcggtgatc
    11101 gggccggttg aaatgcctgc gcagcagctc ggcgaattct ccgagcagct ttttctcgtc
    11161 gtcgcctgca aacgatttga tgcggaactg ttccgccccg ccggcgtgct tgaggaaccc
    11221 tacggaaatg cagatgatct ttccgaactc ggcgtaaatg ccggcgcggt tgtaaagcgt
    11281 ttcgggcgta tcgtcttcgc ttttcttcag aagggccgct ttgaggtccc ataatttttt
    11341 ccattgttcc ggcagaagat cataagaagc atacgcagga acggtttcga tgtcgagaaa
    11401 taaaatgttc tcgggctgca aggtgttgta catactggta aaattaaaag ttacaaaagc
    11461 tgaaaattgc aaagtttttt ccggaaggcc gcaaaggaat gaaatgaccg ggaaataaag
    11521 ccgtttgcaa acgaatataa gcggatataa acgaaaaata tccgtcgcat ggttcctgtt
    11581 caaattgctc cgggcggaaa cctcaccgga tcatcacgcc ttcgatattg atgagcggga
    11641 ctttggtgaa attcttttcg ctgatggatt cgggaagaaa aatgtaacgt ttgtcgtaga
    11701 tctgctcccc gatgtaaaag ctcacccagt attcattgct gagcccgaag gtaaaatcca
    11761 tgatcggttc gattttcgca aaatcgttcg cggggatttc cgtgaagaaa tggcgcaacg
    11821 tgctggtgct gcgtttcgct ccgtccatct caccatagcc tttcgacgta acgagcacgt
    11881 tgcggatggg aacgtctttc atgttcacga tgtaaacgtt ccacacattt tcgccgttgt
    11941 cgtcctcgag gatcactgca atggccacat cttcgacgac gggaatctcg atgtcttttt
    12001 tcatacgctg ctgcaaagat acgatgtgca gtccgctgtt cccgcaccgg gatttcccgc
    12061 gcagctcagc tgccgtacat ctgcagcgcg gcattgaggt aataatgcac gtccagcgga
    12121 agatcgttgg aatcgttggg caggcgtttc tgcccgagac gcacgatgat catcttcagg
    12181 tcgggaacaa caatgatgta ctgcccgagg atcccgcgcg cgtaaaaaat gtggtgcccg
    12241 gcgtattccg ggatcatcca ccagttgtag ccgtaggtga ggcagggtct tccaccgaaa
    12301 tcgtgcatgc cggtaggctg aacggacgcc agcgcgtagc tttcgggaac aagctggttg
    12361 ccgttccatc ttccgctgtc gagatacaat ttgccgagac gggcgaaatc acgcgcattc
    12421 gaattgaagc agcagaatgc tttttcatcg ccgtccttgt gatcgaggct ccagaaagcg
    12481 ctgtgctcgc aacccatggg aatccacaat ttttcagagg cgtaatccgc gaggtgcttt
    12541 cccgtcgctt tttcaacgac aaaaccgagc agctgcgtgt ttccgctgag gtataaaaaa
    12601 ttatcgccgg gcgttccgtt cattttcttg tagccgaacg taagcgtacg caggtcgctg
    12661 ccgtaatacg cttcagccgg gtaggcaagc gggttcacgt aatcttcgtc gaacgcgatg
    12721 cccgagctca tctcgagcag gttcttcagc gtgatctttt cgaactgcgg ctgtttgaat
    12781 tccgggagat aattgcaaac gggatcgtcg acgcttttga tcgcaccgtc ttttatcgcg
    12841 cagccgacaa gaatgctggt gatcgttttc gccatgctga atgaattcgt gtacgaagca
    12901 ttgctgaatt cgccccagta tttttcgcag cggatggaat cgtccttgat caccaggaat
    12961 gcgcctgttt caatttgcat caggctatcg agtattttcc cgggaatttc cttctggttg
    13021 tagtctttcc ccagcgccca gggctgtggc gtcccattct tcacctcgcg gttgtcgaaa
    13081 attttgtact cagtggcgga aggtccttta cgcccgcgga ggtaggtatt ccacacgcct
    13141 ttgtagaggt aaaaccggcc cgtgaaaacg atgaagagat tcaccgcgac gagcaggaag
    13201 aggataaaga ggccaaggcg tttgaggaat tttctgagtt ttttcataca gcggatgtag
    13261 catagcaaat tatgtttttt tacgaagcgc gggaaaagaa gttctggttt gaccgggcgc
    13321 atggaagttt agtcttgcct gaacgaacta aaagccggga aaacctggtc gaagtgttgc
    13381 ctgtcacgct attcttttcc gttctttccg gcacttgatt tatcgcctaa ttttacctcg
    13441 atgaaccgga aacgcatcct tttcctcggc ggagcgctca tcttcctgct gcattttttc
    13501 ttcaccgcgg cttattcgtt cccgggactg gccgggaacc gtttcctgca gcgcgtgacg
    13561 ggtcattaca tgtttcccct gttcaaccag gactggaaaa tattcgcacc cgatccgccg
    13621 gtcttttcga aaaaactgtt ttaccgtgct gcgttttccg acggaacatc ctccggctgg
    13681 accgatcccg gtgcggagct gctggaaaaa cattaccgca accgcttcgg gaactgccgg
    13741 gaccgtttcg ccatttatga agcgatgggc cgcgaaatgg aagccggtga agattcgctg
    13801 cccttgaaat acgtgctgaa cgattgcgca tcgcgtttcg gggcaaagaa aatttccatc
    13861 atcgaatgct gccgtgttta cgtttcggtt ccggaattca aaactggaga gggaggcgga
    13921 atgctcaccc tggaccgcta tttcaaaaaa gacctgcgtg aaaaccattg accggctttc
    13981 ggaacgcttc acctcttccg tcgtgcaggc gggaacgatc gctcttttcc ggaaatgcct
    14041 gtacgcgttc ctgttcttcc gcacgatcac gcttttgccg gcagcgaatg aattctgggg
    14101 gccgggttct tatctgccgt tctattaccg gcagtcttcc ttcctgctga agctgctgaa
    14161 cccgctgcag tcggaagcat ttcatgctta ttatctcttt ttcgcgatcg cacagctggc
    14221 gctgatcgca atttcattct tcgtaaaaaa acgccagcgt cttctctgcg cgggaattta
    14281 ttttttcacg gcagcgcttc accacaaaat ggcggctatt gaaaacggcg gggaacaact
    14341 ggcgatgatc ctgctttttt ttcttttgct gatgaacgaa aacggaaccg gaatgctgaa
    14401 cagcacggtc accaatttcg ctttcctggc ggcgcgcgtg cagttggtgt tcgtttattt
    14461 tgtggcgggg atcaccaagc tgaaaggtgt tcactggata gacggcacgg cgttgcatta
    14521 cgtattcgca acggacgaat at
//
LOCUS       DASTSF010000276         2938 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1986937,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000276 DASTSF010000000
VERSION     DASTSF010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2938
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1986937"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1406)
                     /locus_tag="VFU15_08035"
     CDS             complement(<1..1406)
                     /locus_tag="VFU15_08035"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEU4717768.1"
                     /translation="MANKPWFPFAVFCAGLLLFVPFLGHVHLFDWDEINFAECAREMI
                     VTHDYSTVKIGFLPFWEKPPLFIWMQALCMNIFGIGEFAARLPDALCGAFTLAAVFYL
                     GRKIYDARFGLVWMLAFAGSVLPAFYFHSGIIDPWFNLFIFLGVYQFIVYANDFPSPS
                     TSKLLNGRIILSALFIALAVLTKGPVALLVFGLCFVIARFRARKPIMSWKHLGLYAAV
                     SLLIPGVWFAMLALNGKGNLIVEFLLYQVRLFSTQDAGHGGSFFYHWIVLLIGCFPAS
                     VFAITGLTRKADDTPFELFFLKWMRTLFWVVLILFSIVQTKIIHYSSLCYFPLTFLAA
                     YALRKFESGSLRMKKWTLPALFLSGMIYAAAFFVLPYIDSFKHALLDSGMVKDRFAAG
                     NLAATGHWNGSEWITGVILLAGLVMAVLLFRRNNFQKAVTVLFVSSAVCLFTAAAFFA
                     PRVEEYSQDAAIRYWERHA"
     gene            complement(1396..2046)
                     /locus_tag="VFU15_08040"
     CDS             complement(1396..2046)
                     /locus_tag="VFU15_08040"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEU4717769.1"
                     /translation="MRTAKSLFRESGFFLIPYSAFLLAAGTVLSLYSRREIHLFVNRH
                     YSAAGDLLMPMFTWLGDGYSITVLVLLLFALNRRFAFFTGIACLGASGVTQLLKHTLF
                     YGDPRPMLVFKPGELRLVPGIENDFFNTFPSGHSTVAFAFFFSLSLAFRNKWLKLLMF
                     LFAATIAYSRIYLSQHFLGDVFAGSLIGTSVSFLLFTFASGKKHVEIPIIPKKNGE"
     gene            complement(2077..>2938)
                     /locus_tag="VFU15_08045"
     CDS             complement(2077..>2938)
                     /locus_tag="VFU15_08045"
                     /inference="COORDINATES: protein motif:HMM:NF039344.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="START-like domain-containing protein"
                     /protein_id="HEU4717770.1"
                     /translation="KTKKAEKKPAKKIGKKQVKVSAKPNVKVPAKNIKSSKPEVKAKK
                     GKEKTVAVASAKAPKAVKEKTVKPVVPVAVTVPPVAKKEAAPRPERHRKSQKDQTIVP
                     LDPSIDPTKLPKLPKGIVYKRGKGKLAPGEKRLVKTEVITHHIITDKPLETEKKSKPE
                     PKGKFSMEFVIHTPVALLYDFLTTPNGLAEWFADGVDLKNDVYTFDWDGAKQNAAIVS
                     AKLDSYIRLHWVDKPEGTYFEFRLEQDELTNEVSLIVTDFGENPDDIVTSRQLWEAQI
                     HRLVKALGTY"
BASE COUNT          772 a          763 c          791 g          612 t
ORIGIN      
        1 gcatgtcttt cccaatagcg gatggccgca tcctgcgaat attcttccac gcgcggggcg
       61 aaaaatgccg cagcggtgaa aaggcaaaca gccgaagaga cgaacagcac ggtaaccgct
      121 ttctgaaaat tgttcctgcg aaaaagaaga acagccataa ccaggccggc aagaagtata
      181 acgccggtga tccattcgga cccgttccag tggcccgtgg cggcaaggtt tcccgcggcg
      241 aaacggtctt tcaccatccc ggaatccagc agcgcatgtt tgaacgaatc gatgtacggc
      301 aggacaaaga aagcggcagc gtagatcatc cccgaaagaa aaagcgcggg cagcgtccat
      361 tttttcatgc gcagcgagcc gctttcgaat ttccgcaggg cgtaggcagc gaggaatgtc
      421 agcgggaaat agcacagcga agaataatga atgatcttcg tctgcacgat ggaaaaaaga
      481 ataagcacga cccagaaaag cgtgcgcatc catttgagga aaaacaattc gaacggcgta
      541 tcgtctgctt ttctcgtcag cccggtgatc gcgaaaacgg aagccgggaa gcagccgatg
      601 agcagcacga tccagtgata gaaaaaactt ccgccgtgtc ccgcatcctg cgtgctgaac
      661 agccgcacct ggtaaagcag gaattcaacg atcaggtttc ccttcccgtt cagcgcgagc
      721 attgcgaacc agacgcccgg gatgagcagc gatacagcgg cgtaaagtcc taaatgcttc
      781 cagctcatga tgggtttccg cgcacggaat cgcgcaatga cgaagcaaag cccgaaaacg
      841 agcagggcca ccggtccttt cgtcagcaca gcaagcgcaa tgaacaatgc ggaaagaatg
      901 atccttccgt tcagcagctt tgaagtggaa ggagacggaa aatcattcgc gtaaacgatg
      961 aactggtaaa cgccgagaaa aatgaacagg ttgaaccacg gatcgataat tcccgaatga
     1021 aaatagaaag cggggagaac actcccggca aaagcaagca tccacacgag cccgaaacgt
     1081 gcatcatata ttttccggcc gaggtaaaaa acagccgcga gcgtaaacgc gccgcacagt
     1141 gcgtcgggca ggcgcgcggc aaattccccg atgccgaaaa tattcatgca cagcgcctgc
     1201 atccagataa agaggggagg cttttcccag aacggaagga acccgatctt caccgtgctg
     1261 taatcgtgcg tgacgatcat ttcgcgcgcg cattcggcaa agttgatctc gtcccagtcg
     1321 aagagatgca catgaccgag gaacggaacg aaaagcagca gtcctgcgca gaaaacagcg
     1381 aacggaaacc agggtttatt cgccattctt tttcgggatt atcgggatct cgacgtgttt
     1441 tttcccggaa gcgaacgtga aaagaagaaa cgaaaccgac gtgccgatca gcgaacctgc
     1501 gaaaacgtcg ccgaggaaat gctgggaaag atagatccgt gaataggcaa tggtcgctgc
     1561 aaacaggaac atcagcagct tcagccattt gttccggaac gccaatgaaa gcgaaaagaa
     1621 gaacgcgaac gccaccgtgg aatgtcccga cgggaacgta ttgaaaaaat cgttctcaat
     1681 ccccggaaca aggcgcagct cgccgggttt gaagaccagc atcggccgcg gatcgccgta
     1741 aaacagcgta tgcttgagca actgcgttac cccggaagcg ccaagacagg cgatgcccgt
     1801 gaaaaaagcg aaccttcggt tgagtgcgaa aagcagcagc acgagcacgg taatcgaata
     1861 cccgtcgccc agccaggtga acatcggcat caggagatcg ccggcagcgc tgtaatgccg
     1921 gttgacgaac aggtggatct cgcggcggct gtacaggctg agcactgttc cagcggcaag
     1981 aaggaatgca gagtagggaa tgagaaaaaa tccagactca cggaaaagag atttcgctgt
     2041 gcgcatcggc actgcaaaaa taaggcttga atcggatcag taagtaccca gcgcctttac
     2101 gaggcgatga atctgcgctt cccagagctg gcgggaagtg acgatgtcgt cgggattttc
     2161 accgaaatcc gttacgatca gtgacacttc attggtaagt tcgtcctgct cgagacggaa
     2221 ttcgaaatag gttccttcgg gcttatccac ccagtggaga cgtatatagg aatccagctt
     2281 cgccgaaacg atggcagcat tctgtttggc accgtcccag tcgaacgtat aaacgtcgtt
     2341 cttgagatcg acgccgtccg cgaaccattc cgccaggccg ttcggcgtag tgagaaaatc
     2401 atacagcagc gcaacaggcg tgtgaatgac aaattccatg gagaatttcc ctttcggctc
     2461 cggtttgctt ttcttctcgg tttcgagcgg cttgtcagtg atgatatgat gggtgatgac
     2521 ttccgtttta acgaggcgtt tttctcccgg cgccagcttt cctttcccgc gcttgtaaac
     2581 aattcctttg ggcagcttgg gcagcttcgt cgggtcgatc gaaggatcga gcggaacgat
     2641 cgtctggtct ttctggcttt tgcgatgcct ttcgggcctg ggcgccgctt ctttcttcgc
     2701 aacaggagga acagtcacag caaccggaac gaccggcttc actgtttttt ccttgaccgc
     2761 tttgggcgct ttagctgaag cgacagccac cgttttttcc ttgcccttct tcgcctttac
     2821 ttctggtttt gaagacttta tatttttagc cggcactttc acgttcggct ttgcagatac
     2881 cttcacttgc tttttcccta tcttttttgc aggcttcttt tcagcctttt ttgtcttt
//
LOCUS       DASTSF010000277         2947 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1117388,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000277 DASTSF010000000
VERSION     DASTSF010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2947)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2947)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2947
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1117388"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            155..2530
                     /locus_tag="VFU15_08050"
     CDS             155..2530
                     /locus_tag="VFU15_08050"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018476275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HEU4717771.1"
                     /translation="MYVIKRDGSKESVKFDKITARIQKLCYALDPVHVNPVEVAMKVI
                     QGIYPGVTTSELDNLAAETAASMTTKHPDYALLASRIAVSNLHKNTEKSFSKTIELLY
                     NYVDPKTNQRASLIADDVFEIIRNNAQLLDSSIIYDRDFGYDYFGFKTLERSYLLKID
                     GKIAERPQHMLMRVSVGIHKEDIESAIETYNLMSERWFTHATPTLFNAGTPKPQMSSC
                     FLLQVKEDSIDGIYDTLKNCAKISQSAGGIGLSIHNVRATGSYIKGTNGTSNGIIPML
                     RVYNDTARYVDQGGGKRKGSFAIYLEPWHADIFDFLDLRKNNGKEEMRARDLFTSLWI
                     PDLFMKRVKENGQWSLFCPNEAPGLHECWGEKFETLYTKYETEGRQRRTINAQDLWFA
                     ILESQIETGNPYMLFKDAANRKSNQQNLGTIKSSNLCTEIIEYTSADEVAVCNLASLA
                     LPRFVDNGQFDHQRLFEVTYSVTKNLNKIIDGNYYPIPEARRSNMRHRPIGLGIQGLA
                     DTFILMRYPFDSPEARQLNSEIHETIYYAAMTASKDLAKKDGPYETFAGSPVSKGIFQ
                     FDMWNVTPSSRWEWDILREEVKTHGVRNSLLLAPMPTASTSQILGNNECFEPYTSNIY
                     TRRVLSGEFVVVNKHLLKDLVKLGLWNDGLKNKIIAANGSIQEIPEIPQNIKDLYKTV
                     WEISQRSLIDMAADRGAFICQSQSLNLFVSNPNFAKMTSMHFYAWEKGLKTGMYYLRT
                     KAAADAIKFTVDQNALQNKPEESEAGKSVEEGMSEIACSLDNPDACEACGS"
     gene            complement(2613..>2947)
                     /locus_tag="VFU15_08055"
     CDS             complement(2613..>2947)
                     /locus_tag="VFU15_08055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU4717772.1"
                     /translation="RCIPMTPEREEMEHILQECGFSDVQTENYFVRDDLQDHFLYSFR
                     NFPERYLSEEVRRNTSAFSVFSDPEEVERGVSLLEEEIRSGKIRDVMRAYENGNGDYL
                     FYIARKKQ"
BASE COUNT          771 a          862 c          734 g          580 t
ORIGIN      
        1 gaaggccggc gtcacttccg accgcagcca gaacatattc agcctcgacg aagattttta
       61 attactaacc gcggttatta attgataagc gcggttaaaa ccatcaaccg aattctcctt
      121 ttcccaataa aacccaattt aaattcgctc gtttatgtac gtaatcaagc gcgacggcag
      181 caaagagtcc gtgaagttcg acaagatcac ggcacgcatc cagaaactct gttatgcact
      241 cgacccggtg cacgtgaacc cggtggaagt agccatgaag gtgatccagg gcatttaccc
      301 cggcgttacc accagcgagc tcgataacct cgcggcggaa acggcggcgt cgatgacgac
      361 caagcacccg gactatgcgc tgctcgcctc acgcatcgcg gtctcgaacc tgcacaagaa
      421 cactgaaaaa tccttctcga agacgatcga gctcctgtat aactacgtcg atccgaaaac
      481 gaaccagcgc gcatcgctga tcgccgacga cgtgttcgag atcatccgga acaatgcgca
      541 gctgctggat tcgtccatca tctacgaccg cgatttcggg tatgattatt tcgggttcaa
      601 gacgctcgag cgctcctacc tgctcaagat cgacgggaag atcgcggaac gcccgcagca
      661 catgctcatg cgcgtgtccg tgggcatcca caaggaagac atcgaatcgg cgatcgaaac
      721 ctacaacctc atgagcgagc gctggttcac gcacgccacg cccacgctct tcaacgcggg
      781 cacaccgaag ccgcagatgt cgtcctgctt cctgctgcag gtgaaagaag acagcatcga
      841 cggcatttac gatacgctga agaactgcgc gaaaatttca caaagcgcgg gcggcatcgg
      901 cctgagcatc cacaacgtgc gtgcgacagg atcgtacatc aagggaacga acggaacatc
      961 caacggcatc attcccatgc tgcgcgtgta caacgacacc gcgcgttatg tcgaccaggg
     1021 cggcggaaaa cgcaaaggct ctttcgccat ctacctcgaa ccgtggcatg ccgacatttt
     1081 cgatttcctc gacctgcgca agaacaacgg gaaagaggaa atgcgcgcac gcgatctttt
     1141 cacctcgctc tggatcccgg accttttcat gaagcgcgtg aaagagaacg gacagtggtc
     1201 gctgttctgc ccgaacgaag cgccgggtct gcatgaatgc tggggtgaaa agttcgaaac
     1261 gctttacacg aaatacgaaa ccgaaggacg gcagcgcaga acgatcaatg cgcaggacct
     1321 ctggttcgcc atcctcgaat cgcagatcga aacgggcaac ccgtacatgc tgttcaagga
     1381 tgccgccaac cgcaaaagca accagcagaa cctcggcacg atcaaatcat ccaacctctg
     1441 caccgagatc atcgaataca cttccgccga tgaagtggcc gtgtgcaacc tcgcatcgct
     1501 cgcccttccg cgtttcgtcg acaacgggca gttcgatcac cagcgcctgt tcgaggtgac
     1561 gtattccgtg acgaaaaacc tgaacaaaat catcgacggg aattattatc ccatcccgga
     1621 agcgcgccgt tccaacatgc gccaccgccc catcgggctg ggcattcagg gacttgccga
     1681 tacgttcatc ctcatgcgct acccgttcga ttctccggaa gcgcgccagc tgaattccga
     1741 gatccatgaa acgatctatt acgcggcgat gacggcatcg aaagacctgg caaaaaagga
     1801 cgggccgtac gaaacattcg ccggttcccc ggtgtccaaa ggaattttcc agttcgatat
     1861 gtggaacgtc acgccgagct cacgctggga atgggacatt ctccgcgaag aagtaaaaac
     1921 acacggcgta cgcaattcgc tcctgcttgc accgatgccg acggcatcca cctcgcagat
     1981 cctcggcaac aacgaatgct tcgagccgta cacgtccaac atttacacgc gccgtgttct
     2041 ttccggtgag ttcgttgtcg tgaacaagca cctgctgaaa gacctcgtga agctggggct
     2101 gtggaacgac gggctgaaga acaagatcat cgcagccaac ggttcgatcc aggagatccc
     2161 ggaaattccg cagaacatca aggacctgta caaaaccgtc tgggaaattt cccagcgctc
     2221 gctgatcgac atggccgccg accgcggcgc attcatctgc cagtcacagt cgctgaacct
     2281 gttcgtgtcc aatcccaatt tcgcaaaaat gacatccatg catttttacg cgtgggaaaa
     2341 aggactcaag accggcatgt attacctgcg cacaaaagca gccgcagacg cgatcaaatt
     2401 caccgtcgac cagaacgcgc tgcagaacaa accggaggaa agtgaagccg gaaaatcggt
     2461 ggaagaagga atgtcagaga tcgcctgttc gctcgataac ccggatgcgt gcgaagcctg
     2521 cggaagctga tgtgaagatt cagattcaag gttaatggta aaaaaatcct cctgcacgcg
     2581 caggaggatt ttttatgcag atcgcttcca ctttattgtt ttttccgcgc gatataaaac
     2641 aaataatcgc cgttgccatt ttcataagcg cgcataacat cccggatctt tcctgaacgg
     2701 atttcctctt ccagcaggct cacaccacgt tccacttctt ccggatcgga aaacacgctg
     2761 aaggccgacg tattgcgccg cacctcttcc gacaaatagc gctcgggaaa attcctgaag
     2821 gaatacagga agtgatcctg cagatcatcg cggacaaaat agttctcggt ctgcacgtcc
     2881 gaaaaaccgc attcctgcag gatatgctcc atctcttcgc gttccggcgt catcgggata
     2941 caacgtt
//
LOCUS       DASTSF010000278         3962 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_58677,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000278 DASTSF010000000
VERSION     DASTSF010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3962)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3962)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3962
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58677"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..758)
                     /locus_tag="VFU15_08060"
     CDS             complement(<1..758)
                     /locus_tag="VFU15_08060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717773.1"
                     /translation="MRHLFVFLLLCGQLPAQVLSKEETLNNHIRSRTQTIAYPSLLKS
                     SVVKDIYDTAARLIEEDRISLSDERKTDETRTLFSFCDDGSLGYTLTYRNDTLTDSTV
                     IAHAFPAYETRSYLHGRLYACEQWSGDSLHRKIVRMTLSSNDTFRDVREIFNTYGPGG
                     KRMERNVIEVYSWYGRTDTISDEKTRYFGDLHTRITDRFDNSGYGNYEWSRDSIRKTE
                     TFVFFSAKGKITGKNVICFSDSGKVLRDTWLDKKG"
     gene            925..2157
                     /locus_tag="VFU15_08065"
     CDS             925..2157
                     /locus_tag="VFU15_08065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717774.1"
                     /translation="MNTGRFLFIFFGFFFACSAFAQSPPPTEHYWTFGFNLGTFGWSP
                     TTSSQLDGNDHFTRVFGGGVGLLTDVSGRQHFSIDANTTGGISAGFLFRDGHTKQFSA
                     VQAEFQLNRAIYEFDPPFHFTVQSDSFARWVMTDRYMKYAVAFQQCIYRGPSSGMGGE
                     SFFYVRESFGQTFYHRNFTDRVQQGHFEDWTDHGTGMTSTTVKANATSLMLGSEIGIR
                     SFSQDHSRTLDFGIVFYAPFTASYTERYEFFRNGASSGKSDVTFNGSTIMLNLRYSFD
                     VKGKIKAIDTTSYHPKDMLVHVDTSGRKMEVQSTFTAHGRTVKVKVWDNDEVDGDSIT
                     LFLNGQIVKEHVSLKRRKKTFRVHLDPGDNYLMMYAENLGTTPPNTAAVEIKEGMHKR
                     RVVLVSDKGKSGAVKIVR"
     gene            complement(2664..3527)
                     /locus_tag="VFU15_08070"
     CDS             complement(2664..3527)
                     /locus_tag="VFU15_08070"
                     /inference="COORDINATES: protein motif:HMM:NF025420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4249 domain-containing protein"
                     /protein_id="HEU4717775.1"
                     /translation="MKKLFFILPVAVLLVSCEKDINISLPEPEKKIVVDGGISTGEVA
                     EINLTWSTGYFDPVDSSSLASYLITSATVTVTDGSLVDTLQLSYDANRPIPIVWKGNT
                     ITGQQGHTYTLNVTAEGKTVSAVTSIHAPIPLDSTWFMIEGGKDSLGFAWAHLTDPPE
                     TGNAYRWFAKREGVDPGFIAPFGSTFDDKFVNGKSFDFAYNRPSPPNSTAPEDNNAEA
                     GYYKVGDTIVVKFCTIGQAESDFFRTYEVEVSNNGNPFASPGVIKTNVTNGLGIWCGY
                     APTYDTIVCHP"
     gene            complement(3533..>3962)
                     /locus_tag="VFU15_08075"
     CDS             complement(3533..>3962)
                     /locus_tag="VFU15_08075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU4717776.1"
                     /translation="FYARYDRRHDLSVVLVYTLNDHWTFGSVFVYGTGNAITLPVARY
                     IIEGQIVSDYGPRNSFRMAPYHRLDISVTYTCKPKKLFKRLPYQSNWNFSVYNVYNRH
                     NPYFIYFDNEGSIADGTLKVTAKQVSLFPILPSVTWNFSF"
BASE COUNT          948 a          977 c         1072 g          965 t
ORIGIN      
        1 ccttttttgt cgagccacgt gtcgcggagc actttgccgc tgtcgctgaa acagatcacg
       61 ttcttcccgg tgatctttcc ttttgcagaa aagaaaacaa aagtttcggt tttccggatg
      121 ctgtcccggc tccattcata attgccgtac ccggagttgt cgaagcggtc ggtgatgcgc
      181 gtgtgaaggt cgccgaagta gcgtgttttt tcatcgctga tcgtatccgt ccgtccgtac
      241 cagctgtaaa cttcgatcac gttgcgttcc atgcgtttgc cgccgggtcc gtaggtgttg
      301 aaaatttcgc ggacgtcccg gaaggtgtca ttcgaagaca gcgtcatgcg cacgatcttc
      361 cggtgaagcg aatcgccgct ccattgttcg catgcgtaca ggcgaccgtg caggtaagac
      421 cttgtttcgt aagcgggaaa cgcatgggcg atcacggtgc tgtccgtgag cgtatcgttc
      481 cggtaggtga gcgtgtagcc gagcgacccg tcatcgcaaa aggaaaaaag agttctcgtt
      541 tcatccgttt tccgttcgtc ggacagagag atgcggtctt cctcgataag ccgggcagcg
      601 gtatcgtaaa tgtctttcac aacgctgctt ttcaggagcg aaggataggc gattgtctgc
      661 gtacgcgagc ggatgtgatt gttcagggtt tcctcttttg aaaggacctg tgcaggaagc
      721 tggccgcaga ggaggaggaa aacgaataag tgccgcataa aacgtttctg taaaaacgcg
      781 ggatctgcaa aaagttacag gccaccgaat ggaaatgaag acgcaccgcc tgcaaaacgg
      841 cattgatttt gcaaaaaata aagcagtaat ttcagcacgg ctcccacgca acgaatgatg
      901 gacaaacgcg tcttcacaaa aagcatgaat accgggagat ttcttttcat cttcttcggt
      961 tttttcttcg cctgcagcgc cttcgcacag tcgccgccgc caacggagca ttactggaca
     1021 ttcgggttca acctaggaac attcggctgg tcgccaacga cgagcagcca gctggacggt
     1081 aatgatcatt tcacgcgcgt gttcggcgga ggtgtcggac tgctcacgga cgtcagcggg
     1141 cgccagcatt tttccatcga tgccaatacg acgggtggca tttctgccgg cttccttttc
     1201 cgcgacggtc atacaaaaca attttcagca gtgcaggccg aattccagct caaccgcgcc
     1261 atctatgaat tcgatccgcc gtttcatttc accgtgcagt cggattcctt cgcacgatgg
     1321 gtgatgaccg accggtatat gaaatacgcg gttgcgttcc agcaatgcat ttaccgcgga
     1381 ccttcttccg gcatgggcgg cgaatcgttt ttttatgtgc gtgaatcgtt cgggcagaca
     1441 ttttaccacc gcaacttcac ggaccgcgtt cagcagggac atttcgagga ctggaccgat
     1501 cacgggacag gcatgacgtc gaccacggtg aaagcgaatg ccacgagcct catgctcggg
     1561 tcggagatcg ggatacgttc tttttcccag gatcattcgc gtacgctcga tttcggaatt
     1621 gttttttacg caccgttcac cgcatcgtac acggaacgct atgagttttt caggaacgga
     1681 gcttcgtccg gcaaaagcga cgttacattc aacggctcga cgatcatgct caatctccgg
     1741 tactcgttcg acgtgaaagg aaaaataaag gcgatcgaca cgacgtctta ccatccgaaa
     1801 gatatgcttg tacacgtcga tacatcagga cggaaaatgg aagtgcagag cacgttcacg
     1861 gcgcacggcc gcacggtaaa agtaaaagtg tgggacaacg acgaagtgga cggcgacagc
     1921 atcacgcttt tcctcaacgg gcagatcgtg aaagaacacg tgagcctgaa acgccggaag
     1981 aaaaccttcc gcgttcatct cgatcccggt gataattacc tcatgatgta cgctgagaat
     2041 ctcggcacga caccgcccaa cacagcggcc gtggagatca aagaaggcat gcacaagcga
     2101 agagtggtgc ttgtttccga caaaggaaaa agcggggcgg tgaagattgt caggtgagga
     2161 gatttatgat ttaagaattg cgatgtacag gcgagaaacg gttatgcccg tttgtaagcg
     2221 tttaatgtgt tggaccacag gatgttttcg gggttccgga gccgctgatt tcaagggaat
     2281 tagttcggta agttgtaata atatacaacc ttgcaggttc taaccttgca ggtggtgcta
     2341 agtgttcggt tctaatccaa cgcgtcatgg caaaatagca cttctcggat gtttcaaata
     2401 attgatttca aatattttat gcttataagt tatattaaca tacaaccttg caggtcctaa
     2461 ccttgcaggt tgccgaacga cctttttctt gttttgacca tataaattat ggtaaatcac
     2521 tgattttgca cgaattagtt tatgtagttg taatactata caaccttgca ggttctaacc
     2581 ttgcaggttg taattctaaa caccccacac aatcccctca ttgccttcca aacatatcct
     2641 gtaacaaaaa agaaaccaca tctctacgga tggcacacga tcgtatcata cgtgggtgcg
     2701 tagccgcacc agatacccag tccgttcgtg acgttcgtct tgatcacgcc gggagaagcg
     2761 aacgggtttc cgttgttgct tacttccacc tcgtaggtgc ggaaaaaatc ggattcggcc
     2821 tggccgatgg tgcagaattt cacgacgatc gtatcgccga ccttatagta gccggcttcc
     2881 gcgttgttat cttcgggtgc ggtggagttc ggcggactcg gccggttgta ggcgaaatca
     2941 aagctcttgc cgttgacgaa cttgtcatcg aacgtggagc cgaaaggcgc tatgaaacca
     3001 ggatccacgc cttcgcgttt ggcgaaccag cggtaggcat tccctgtttc cggcggatca
     3061 gtcagatgcg cccaggcgaa ccccagcgaa tcctttccgc cctcgatcat gaaccacgtg
     3121 ctgtcgagcg ggatgggcgc atgaatgctc gtgacggcag aaacggtttt tccttccgcg
     3181 gttacattga gcgtgtacgt atgtccctgc tgcccggtga tcgtatttcc tttccacacg
     3241 atcgggatcg gcctgttcgc atcgtaggaa agctgaagcg tgtcgacaag tgagccgtcg
     3301 gtcactgtca cggttgcgga agtgatgagg tagcttgcga gcgaagagga atcgacagga
     3361 tcgaaatacc cggtgctcca ggtgagattt atttccgcca cttccccggt ggaaattccc
     3421 ccgtcgacga cgatcttctt ctccggttcc ggcaaagaaa tgttgatgtc cttttcgcag
     3481 gagacgagaa gaacggcgac cggcaggatg aagaataatt ttttcatgcg catcagaagc
     3541 tgaagttcca ggtgacggaa ggaaggatag ggaacaggga aacctgcttg gccgtgactt
     3601 tcagtgtgcc gtccgcgatg gagccttcgt tgtcgaaata aatgaaatac gggttgtggc
     3661 ggttgtacac gttgtacacc gaaaaattcc agttgctctg gtagggaaga cgcttgaaca
     3721 gttttttcgg tttgcacgtg taggttacgg aaatgtcgag acggtgatac ggcgccatgc
     3781 ggaagctgtt gcgcggaccg taatcggaaa cgatctgtcc ttcgatgatg taacgcgcca
     3841 ccggcagcgt gatcgcattt cccgttccgt aaacgaatac gcttccgaat gtccagtgat
     3901 cattcagcgt gtaaacgagc acgacggaaa gatcgtgccg gcgatcgtag cgcgcgtaaa
     3961 at
//
LOCUS       DASTSF010000279         4032 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1505391,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000279 DASTSF010000000
VERSION     DASTSF010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4032
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1505391"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(209..1342)
                     /locus_tag="VFU15_08080"
     CDS             complement(209..1342)
                     /locus_tag="VFU15_08080"
                     /inference="COORDINATES: protein motif:HMM:TIGR01053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717777.1"
                     /translation="MSNDFNEKTHDVAEQGKVKCKECGGLLMYNPGVQSLKCEYCGTV
                     NDISEEMKPVQAEEIDFLAFLANAENTAEKMEITTVKCDSCGSSTTLSPNVTADTCPF
                     CGTALVVKGGTTSSLIRPKYMLPFKIDKKKGTEEFKKWLSGLWFAPGDLKNAAQNDKL
                     KGLYIPYWTYDTNTVSDYTGLRGMYYYETETYTVTENGKTETRTREVRKVRWEPANGT
                     VYDSFDDVLVIASSSLPEKYANELEPWDLDQLAAFNEQFLSGFIAEKYQLDVKGGFEK
                     AKVKMDPVIRTTVRKNIGGDEQQITTLNVIYNDITFKHILLPVWISAFKYNSKVYRFM
                     VNGRTGEVQGERPYSFWKIFGAVAGGLAIIAGAVYLYMKYKHG"
     gene            complement(1350..1733)
                     /locus_tag="VFU15_08085"
     CDS             complement(1350..1733)
                     /locus_tag="VFU15_08085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717778.1"
                     /translation="MARTVRSLMDMLTSLLSEQEDDLSGAEEFCRFYAETKNYLVRYA
                     EQTMSTFIAQKTASLPAIQPAEFRKGNDFGIFGLLLSTRLRAFRVNEKLEMKLRQIRN
                     DLTAILAVIESPELENMYNKSILTK"
     gene            complement(1830..2966)
                     /locus_tag="VFU15_08090"
     CDS             complement(1830..2966)
                     /locus_tag="VFU15_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007281030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEU4717779.1"
                     /translation="MGLLDKLKGEFIDIIEWTDSSNDTIVYRFPRYNNEIKNGAKLTV
                     RESQVAVFVNEGQMADAFLPGMYELKTQNLPILSTLKGWKYGFDSPFRAEVYFVNTKQ
                     FTNLKWGTKNPIMIRDAEFGPVRIRAFGSYAMKVKDAKTFLKEVAGTDNEFTTDEINE
                     QLRNIVVTRFTDGVASSKIPILDMASNYDEYSKLIMDKIAPEFNEYGIELTKLLVENI
                     SLPPEVEAMLDKRSSMGILGNLGAYSQFQSANAMEAAANNPNAGGIMGAGVGMGMGFG
                     MGNQMSNMYQNNQFNPQTGMQNNQQGPPPPPPVIQYFVAVNGQQQGPYDENAFRTMIQ
                     SGAVKRETMVWKNGMAAWTAAGQVPELAALFSMTPPPPPPPPVG"
     gene            3167..>4032
                     /locus_tag="VFU15_08095"
     CDS             3167..>4032
                     /locus_tag="VFU15_08095"
                     /inference="COORDINATES: protein motif:HMM:NF015451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacetone oxidase family FAD-binding enzyme"
                     /protein_id="HEU4717780.1"
                     /translation="MRNTLDLLVIGGGAAGFFAAANVAEQDPSLRVAILEKTGKLLSK
                     VRISGGGRCNVTHACFDNRLLSEFYPRGNRELLQAFSRFSPAGTIEWFSSHGVELKTE
                     EDGRMFPVTDNSLSVVSCLTEVIHRNNVEVHLHCGVEKISKKENFFEVTAGGSVFHSE
                     NVLIAAGGNPSSGFYDFIRETGHVIVPPVPSLFTFNIRSAELASLMGISVPGASVEIK
                     EANIFTEGPVLITHWGLSGPAILKASSLGAKWMADRQYNFEAAVNWLNPAEAETVFEE
                     LKWIKMQHPRQKV"
BASE COUNT          847 a         1052 c         1079 g         1054 t
ORIGIN      
        1 cctgcttgcc ctgctgctgc agccattcct gcgcacggtc gtttcctttg atcgcatcag
       61 caagatgctg tatgtgctgg tcgttaatga aagacatttc tgttctgaat ttaaaattcc
      121 ggggtgaacg ttccgttgcg aaccttcatc gctgaaaagg tgttttcatg caaagatgca
      181 gaggaacttc caacccggag gtcaaagatc acccgtgctt gtatttcatg tacaggtata
      241 ccgcaccggc aataatggcg aggccgccgg caaccgctcc gaatattttc cagaagctgt
      301 acggacgttc gccctgcact tcgccggtcc ttccgttcac catgaagcgg taaactttgc
      361 tgttgtactt gaacgcgctg atccagacag gcagaagaat gtgcttgaac gtgatgtcat
      421 tgtaaatgac attcagcgtg gtgatctgct gctcgtcgcc gccgatgttt ttccgtacgg
      481 tggtccggat gacgggatcc attttcactt tcgccttttc gaagccgcct tttacatcga
      541 gctggtattt ttcggcaatg aagccgctga ggaattgctc attgaaagcg gcgagctggt
      601 caagatccca gggctcgagc tcgttcgcat atttttccgg gagtgaactg gaagcgatca
      661 cgagcacatc atcgaatgaa tcgtataccg taccgttcgc aggttcccag cgaactttcc
      721 gcacttcacg tgtgcgggtt tccgttttgc cgttctcggt aacagtgtat gtttccgttt
      781 cataataata catcccgcgc agaccggtgt aatcactcac ggtattcgtg tcgtacgtcc
      841 agtagggaat gtaaagtcct ttcagcttgt cgttctgcgc cgcatttttc aggtcgccgg
      901 gagcgaacca gagtccgctg agccactttt tgaattcttc tgttcctttc tttttgtcga
      961 tcttgaacgg gagcatgtat ttcggccgga tgaggcttga agtggttccg cctttcacaa
     1021 cgagcgccgt gccgcagaac gggcacgtgt cggccgtgac attcggggaa agcgtagtgg
     1081 acgatccgca gctgtcgcat ttcacggtgg tgatctccat cttttcggcc gtattctccg
     1141 cattggcgag gaacgcaaga aaatcgatct cttcagcttg taccggtttc atctcttccg
     1201 aaatgtcatt caccgttccg cagtattcac acttcaggct ctgcacaccg ggattgtaca
     1261 tcagcagtcc gccgcattcc ttgcatttca ctttgccctg ttcggcgaca tcgtgcgttt
     1321 tttcattgaa atcgttgctc atgggtttgt tatttggtca ggatggattt gttgtacata
     1381 ttttccagct cgggactttc gatcacggcg agtatggcgg tgagatcatt ccttatctgc
     1441 ctgagcttca tttcgagttt ttcattcacg cggaacgcac gcaggcgggt ggaaagcagc
     1501 aacccgaaaa ttccgaaatc gtttcctttc cggaactcgg ccggctggat agccgggagg
     1561 gatgccgttt tttgtgcgat gaacgtgctc atggtttgct ccgcataacg gaccaggtaa
     1621 tttttcgtct cggcgtaaaa ccggcagaat tcttccgcgc ctgaaagatc atcttcctgc
     1681 tccgaaagaa gagacgtcag catgtccatg agcgaacgca cggttcgcgc catggattcc
     1741 ggttttatgg tacggggtat cattcggcta agaaagaaaa aatgccggtg cagtgaaaaa
     1801 cctgcaaccg gcaattgaat tttcccggat tatcccacgg gcggcggcgg gggaggagga
     1861 ggcgtcatgc tgaacagcgc cgcaagctcc ggaacctgcc cggcagcggt ccatgcagcc
     1921 atcccgtttt tccagaccat cgtttcgcgt ttcaccgcgc cgctctggat catcgtgcgg
     1981 aatgcatttt catcgtaagg tccctgctgc tgcccgttca ctgcaacgaa atactggatg
     2041 accggcggcg gaggaggagg accctgctgg ttgttctgca tgcccgtctg cgggttgaac
     2101 tggttgttct ggtacatgtt cgacatctgg ttgcccatgc cgaatcccat gcccatgcct
     2161 acgcctgcgc ccatgatgcc gcctgcgttc gggttgttcg ctgccgcttc catggcgttg
     2221 gccgactgga actggctgta tgcaccgagg ttgccgagaa tgcccatgct ggaacgcttg
     2281 tcgagcatgg cttccacttc gggcggcagg gaaatatttt ccacgaggag cttcgtcagc
     2341 tcgatgccat attcgttgaa ttcaggcgcg atcttgtcca tgatcagctt cgaatactca
     2401 tcgtaattcg aggccatgtc gaggatcgga attttgctgg atgcgacgcc gtcggtaaag
     2461 cgcgtgacga cgatattgcg cagctgctcg ttgatctcgt cggtggtgaa ttcgttatcg
     2521 gttccggcta cttctttcag gaatgttttc gcgtccttca ctttcatcgc gtacgatccg
     2581 aaagcacgga tacgcacggg cccgaattcc gcatcgcgga tcatgatcgg gtttttcgtt
     2641 ccccatttga ggttcgtgaa ctgtttcgta ttgacgaaat aaacttccgc acggaacggg
     2701 ctgtcgaaac cgtatttcca tcctttcagc gtggaaagaa tgggaaggtt ctgcgttttc
     2761 agctcgtaca tgccgggcag gaacgcgtcg gccatctggc cttcgttgac gaaaacagcg
     2821 acctgcgatt cgcggaccgt cagcttcgcg ccgttcttga tctcgttatt gtaccgcgga
     2881 aaacgataaa cgatcgtgtc gttcgagctg tccgtccact cgataatgtc aatgaactcg
     2941 cctttgagtt tgtcgagaag tcccatgata aattgtgtta caggtttgtg tgttgtttct
     3001 gaaaaatccg cttcgcgggt ctatgatcaa atatagcttt ttttgaaaag tgccggtttt
     3061 aaaaaacgtg aaataatccg gtgatcccgg taaacgacaa cgcgaaaaaa gattccgccg
     3121 cagatcatga acgcagatgc ggatttgcta aacttgcggc ttcctcatga gaaacacgct
     3181 tgatctcctg gttatcggcg gcggcgctgc cggctttttt gcggcagcca atgtggctga
     3241 gcaggaccct tctctccgcg tcgccattct tgaaaagacc gggaagctcc tctcgaaagt
     3301 gcgcatttcc gggggaggaa gatgcaacgt cacacatgcc tgcttcgaca accggctgct
     3361 gtcggaattc tatccccgcg gcaaccggga gctgctgcag gcgttcagcc gtttttctcc
     3421 tgccggcacc atcgaatggt tttcatcgca cggcgttgag ctgaagacgg aagaggacgg
     3481 acgcatgttc ccggtcacgg ataattcgct ttcggtggtc agctgcctga cagaggtcat
     3541 tcaccggaat aacgtggaag tccatcttca ctgcggcgtg gaaaagatca gcaaaaaaga
     3601 aaatttcttc gaagtgaccg ccggaggaag tgtttttcat tcggaaaacg tgctcatcgc
     3661 cgcgggcggg aacccgtctt ccggctttta cgatttcatc cgcgaaaccg ggcacgtgat
     3721 cgttcctcca gttccttcac tgttcacatt caatatccgc tcggcggagc tggcttcgct
     3781 catgggaatt tccgttccgg gcgcttccgt tgaaataaaa gaagcaaaca tttttacaga
     3841 aggcccggtg ctcatcacgc attggggact gagcggacct gcgatcctga aagcgtcatc
     3901 gctcggggca aaatggatgg cggaccggca gtataatttc gaagcagcgg tcaactggct
     3961 caatcccgcc gaagcggaaa ccgttttcga agaactgaaa tggataaaaa tgcagcaccc
     4021 gcggcagaaa gt
//
LOCUS       DASTSF010000280         3888 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_581385,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000280 DASTSF010000000
VERSION     DASTSF010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3888
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_581385"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1419)
                     /locus_tag="VFU15_08100"
     CDS             complement(<1..1419)
                     /locus_tag="VFU15_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013750768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEU4717781.1"
                     /translation="MAQVMTDQFGIAEALKALGISENNYGASTGTKHWSTKGAKVDSY
                     SPADGQFIGSVNQATAEDYENVIRTAGEAFKTWRTIPAPKRGEVVRQIGEQLRKYKEP
                     LGKLVSYEMGKSLQEGLGEVQEMIDICDFAVGLSRQLYGLTMHSERPKHRMYEQWHPL
                     GIVGIISAFNFPVAVWSWNAAIAWVCGDVCVWKPSSKTPLCAVACQNIISSVLKKNNV
                     PEGVSCVLIGNPVGDMMNSDKRVPLVSFTGSTRIGRRVSSMVAERFGKSILELGGNNA
                     LIVSQYADLNMVLVGAVFGAVGTAGQRCTSTRRLIIHESVYEKTISVLTNAYAQLQIG
                     NPLDAKNHVGPLIDKKAVEDYLNAIERAKKEGGKMIVEGGVVSGDGFESGCYVKPCII
                     EAKNDYHIVQEETFAPILYVMKYKTIDEAIAMQNGVPQGLSSSIFTTNMREMEQFLSQ
                     EGSDCGIANVNIGTSGAEIGGAF"
     gene            complement(1504..2118)
                     /locus_tag="VFU15_08105"
     CDS             complement(1504..2118)
                     /locus_tag="VFU15_08105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717782.1"
                     /translation="MKYASPFPAIRNFFAYCLFSWMLLVVAVYPFLDLVHATAKCTSL
                     PLMLSRDANRNFTRQEPAEALQKQVGATVDVSVITGRGVLRNDFPPLFGDAQRLEWLR
                     TAARGFYSLYIAALVFLALGKFSRASVAFAAFSSGVFLLSYVLLDLQAVYFGAATRHF
                     SQYNLEIGFGWETWFSVITIVNTILAVANVALLLFRPGTKRSGD"
     gene            complement(2132..3586)
                     /locus_tag="VFU15_08110"
     CDS             complement(2132..3586)
                     /locus_tag="VFU15_08110"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HEU4717783.1"
                     /translation="MDRNARELSLLRQKIKTLEKENAALREQHKAFTTSLPTVKSPDS
                     FRPLFNDAEKTVREYFSDFHPDPSRGTIDISGERYLLVRASALSVEFLNSIKSLYRDR
                     GEEEAYAIGRNFLFDIAHVIGKEDAKSFHSKMKLTDPIAKLSAGPVHFAYTGWAFVDI
                     LPESRPSPDENYFLSYRHPYSFEADSWINAKKKAKFPVCIMNAGYSSGWCEESFGVEL
                     TAVEVTCRAMGHESCTFIMAPPSRIQHYLKTHKAFVHSKKQLRVPHFFERKKIEEDLE
                     ASVKEKELLLKEIHHRVKNNLQIISSLLSLQSMSITDKKLKQKFRESQDRIRSMALLH
                     EALYSTHEFDNVSSRHYFRALTRSLYGSYGKSEKEISLSVDIGKSSDRMTIDAAIPCG
                     LIINELISNSLKYAFPGGKQGSIYVKLNAKGHHPAKYTLIIGDTGRGLPKRVLTGEPE
                     SLGLQLVFALVEQLSGSVIINNGNGAEFIITFEA"
BASE COUNT          846 a         1052 c         1047 g          943 t
ORIGIN      
        1 gaacgcaccg ccgatctccg caccgcttgt tccgatgttc acgttcgcga tgccgcagtc
       61 gcttccttcc tgtgaaagga actgctccat ctcgcgcatg ttcgtcgtga agatggaaga
      121 agaaagtccc tgcggaacgc cgttctgcat cgcgatcgct tcgtcgatgg ttttgtattt
      181 catcacgtac aggatgggcg cgaacgtctc ttcctgtacg atgtggtaat cgttcttcgc
      241 ctcgatgatg cacggcttca cgtagcatcc gctttcaaat ccgtccccgc ttaccacgcc
      301 gccttcgacg atcatcttcc cgccttcttt cttcgcacgt tcgatcgcgt tgaggtaatc
      361 ttccaccgct tttttgtcga tcagcggacc gacgtggttt ttcgcatcga gcgggttgcc
      421 gatctgcagc tgcgcgtacg cattcgtaag cacgcttatg gttttttcgt aaacgctttc
      481 atggatgatg aggcgccgcg ttgaagtgca gcgctgtcct gctgttccca ccgcgccgaa
      541 caccgcgccc acgagcacca tgttcaggtc ggcgtattgc gaaacgatga gcgcgttgtt
      601 tccgccgagc tcgagaatgg atttcccgaa acgttcggcc accatcgagg aaacccggcg
      661 cccgatacgc gtcgagccgg tgaaggaaac aagcggaaca cgcttgtcgc tgttcatcat
      721 gtcgccgaca gggttgccga tgagcacgca gctcacgcct tcgggaacgt tgttcttttt
      781 cagaacggag gaaataatat tctggcaggc cacggcgcag agcggtgttt tggaagaagg
      841 cttccacacg catacgtcgc cgcataccca cgcgatggct gcattccagc tccataccgc
      901 aacagggaaa ttgaacgcgc tgattatgcc gacaatgccg agcggatgcc attgctcgta
      961 catgcggtgc ttcgggcgct cgctgtgcat cgtgagaccg tagagctggc gggaaagtcc
     1021 caccgcgaaa tcgcagatgt cgatcatttc ctgcacttct cccagtcctt cctgcagcga
     1081 ttttcccatt tcataggaaa ccagcttgcc cagcggctct ttgtatttgc gcagctgctc
     1141 accgatctgg cggaccactt cgccgcgctt cggtgcgggg atcgtgcgcc acgttttgaa
     1201 ggcttccccg gccgtgcgga tcacgttttc gtaatcttcc gccgtcgcct ggttcacgct
     1261 gccgatgaat tgtccgtcgg cgggagaata ggaatccact ttcgcgcctt tcgtgctcca
     1321 gtgtttcgtt ccggtggatg cgccgtaatt gttttcggag atccccaatg ctttgagggc
     1381 ttccgctatg ccgaattgat ctgtcattac ctgggccatg gctgattttt ttaaggaacg
     1441 taaaattacg aaaaaagagg gtgcgggaaa gtgacaggac tcacggatta tacagcgaaa
     1501 tttttagtca ccggatcttt ttgtccccgg gcggaacagc agcaatgcga cattagcaac
     1561 cgcaagaatt gtgttcacta tggtgatcac ggaaaaccag gtttcccacc cgaaaccgat
     1621 ctcgagattg tattgggaaa aatgccttgt cgccgccccg aaatacacgg cctgaagatc
     1681 gagcagcacg taagacaata aaaatacgcc ggaagaaaat gcggcgaacg caacggaagc
     1741 tctggaaaat tttcccagcg cgagaaaaac aagcgccgcg atgtaaagcg aataaaatcc
     1801 gcgtgctgca gttcgcagcc attccaaccg ttgtgcgtcc ccgaaaagcg gtggaaaatc
     1861 gttccgcaaa acaccgcgcc cggtgataac ggaaacatcc accgtcgcgc cgacctgttt
     1921 ctgcaatgcc tcagcgggtt cctgccgcgt gaaatttcta ttggcgtccc tggatagcat
     1981 cagcgggagc gaagtacatt ttgcagttgc atggacgaga tcgagaaacg gatacaccgc
     2041 aacaacaagc agcatccagg aaaacaggca gtacgcaaaa aagttccgga tcgcagggaa
     2101 gggagacgcg tatttcattt tccggaagag gttacgcttc gaatgtaatg ataaactccg
     2161 ctccgttccc gttgttgatg atcacgctgc ccgaaagctg ctccaccagc gcaaacacca
     2221 gctgcagtcc cagcgattcc ggctcgccgg tgagcacgcg tttcggcaac ccgcggcccg
     2281 tgtcgccgat gatcagcgtg tattttgccg ggtgatgtcc ttttgcattc agcttcacgt
     2341 aaatgcttcc ctgttttcca cctgggaatg cgtacttgag tgaattgctg atgagctcgt
     2401 tgatgatcag tccgcaggga atggcggcgt cgatcgtcat gcggtcgctg ctttttccga
     2461 tgtcgacgga aagagaaatt tctttttcgc ttttgccgta cgagccgtaa agcgaacgcg
     2521 tcagcgccct gaaatagtga cgcgagctta cgttgtcgaa ttcgtgcgtg gaatacagcg
     2581 cttcgtgcag cagcgccatc gaccggatcc ggtcctggct ctcgcggaat ttctgcttca
     2641 gctttttatc ggtgatcgac atgctctgca ggctgagcag gctcgagatg atctgcaggt
     2701 tgttcttcac gcggtggtgg atctctttca gcaaaagctc tttttccttc acggaagctt
     2761 ccaggtcttc ctcgatcttt ttccgttcga aaaaatgcgg aacgcgcagc tgttttttcg
     2821 aatgaacgaa ggctttatgc gtcttcaggt aatgctgtat ccgcgaaggc ggcgccatga
     2881 tgaacgtgca gctctcgtgt cccatcgcgc ggcacgtcac ttccactgct gtcaactcca
     2941 cgccgaagct ttcttcgcac cacccggaag aatagccggc attcatgatg cagacgggga
     3001 acttcgcttt tttcttcgcg ttgatccatg aatctgcttc gaacgaatac gggtggcgat
     3061 aggaaagaaa atagttttca tccggcgacg gcctgctttc cgggagtatg tccacgaacg
     3121 cccagccggt atacgcgaaa tgcaccgggc ccgcagaaag tttggcgatc ggatcggtga
     3181 gcttcatctt cgaatgaaag cttttcgcgt cttccttccc gatcacgtgc gcgatgtcga
     3241 acaggaaatt ccggccaatg gcgtacgctt cttcttcgcc gcggtcgcgg taaaggcttt
     3301 tgatgctgtt gaggaattcc accgaaagcg cgcttgcgcg aacgagcagg taacgttccc
     3361 cgctgatgtc gatcgttccg cgcgaaggat caggatggaa atcgctgaaa tattcgcgta
     3421 ctgttttttc ggcgtcgttg aacagcggcc ggaacgaatc cgggcttttg acagtgggaa
     3481 gcgaagtggt gaatgctttg tgctgttcac gcaatgcagc attttccttc tccagcgttt
     3541 tgattttttg cctgagcagg gaaagttcgc gagcgttgcg atccatgaaa taaagtgcag
     3601 gtgatgatca aatgtagaga caatgacgat gcgagtcaag actgctcgta cggccgggga
     3661 ataaaaaacc ggcatgattg aaggttttca gccgggctga ttttttccgc tcagcttcgg
     3721 ttgcagccga agctattcaa tatggatcac gccaaattta ccggcctcac ctttcgatcc
     3781 ataataagaa tgcgtgaatc atacgcctgg agagcgatac ggggccggat aatgacatcc
     3841 gcatccatgg catcaaaaag ggcgttcctg ggcatttcac ggcgctga
//
LOCUS       DASTSF010000281         7495 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1841992,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000281 DASTSF010000000
VERSION     DASTSF010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 7495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7495
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1841992"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1522
                     /locus_tag="VFU15_08115"
     CDS             <1..1522
                     /locus_tag="VFU15_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009282074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HEU4717784.1"
                     /translation="IIVLAIIVTLFLIQRKGTSFIGNLFGPVMLIWFTMIGVLGLGAL
                     MKDFSVLKALNPWWAWRMLSEHKGGFWLLGGVFLCTTGAEALYSDLGHCGRKNIQISW
                     LYVKICLVLCYFGQAAWLMNAHDATLTPFFGIVPHWFFFPAVSIATLATIIASQALIS
                     GSFTLVAEAIRLNVWPKMRIVFPSDIKGQLYIPAINWLLMAGCIGVVLFFKESSEMEA
                     AFGLSVTLTMLMTTILLSFYLYTKKVNRVAVFSLLLIYLLIEGSFLVANLVKFQQGGW
                     ITVLIGSLIFVIMYVWFRAGEIRRSLRETTDLEKFIPVLKELSSDMTVPKYATNLVYL
                     TSSSDENKVEERIIHSILYKQPKRADIYWFVHIEVLDEPYHMSYHVKVYEPDDIIRID
                     FKLGFRVQPRINLLFRKVVEEMVERKEVNINSRYCSLDKHNITGDFRFVIMQRFLSFE
                     NELSFRNEFILKLYFFLKKISLADEKEYGLDYSNVTIEKSPLILSSPKTIPLIRDN"
     gene            1522..2652
                     /locus_tag="VFU15_08120"
     CDS             1522..2652
                     /locus_tag="VFU15_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor protein KdpD"
                     /protein_id="HEU4717785.1"
                     /translation="MEQEEENKEEQSFIDLLKKSKRGKLKIYIGMSAGVGKTYRMLQD
                     AHALVKNGVDVKIGYIETHNRAETHALTEGLPVIPRKKIFYKGKELEEMDLQEILNER
                     PEVVIVDELAHTNIPGSRHEKRWQDVLDLLEADISVITAVNIQHIETISDEVKKIVGI
                     EVKERVPDSFLQMADEVVNIDLTADELITRLKEGKIYDRSKVEQAMKNFFRPENILQL
                     RELALKEVAAQVERKVETEVLTGEKLRHERFLACISSNEEKASRIIRKTARLAAYYNS
                     SWFLLYVQTPHESYDKIPLATQRHLINNFKLATELGAEVIRIKGEDVGQAIMEVAEEK
                     KITTVCIGVPEFRLWQIVLKGGLFNRLLGKLKISDIDLIILS"
     gene            2654..4408
                     /locus_tag="VFU15_08125"
     CDS             2654..4408
                     /locus_tag="VFU15_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013928719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU4717786.1"
                     /translation="MKVTIKAKLRFGLLFLFAVILTIGVLGGLFLYRLSDESGDIIKD
                     NYESVVYTKSMLEACDVLAASPGGEAERRAKEKFSASLRSQMNNITENGEDNLTSQLG
                     AAYSAFEKDTGRAKLNDLFTLRGILYSIQDLNLHAITQKNLRTNETSHTVILWMTIIG
                     TIAVLVSFSFVVNFPGFIANPVRELTSGIKEIAARNYSKRLHFSSNDEFGELAAAFNS
                     MTEKLEEYESSNLAKLLFEKKRLDTVISSMHDAIIGLDENGNILFLNPVAAQLLGVKS
                     ENVTGKQASSVAATNDLLRELTRNIGKESNEKDLSPLKIFADGRESFFNREVLRVTAV
                     PTGESAPQVIGYVIVLKNITQFRELDLAKTNFIATVSHELKTPISSIKMSLNLLEDQR
                     VGMLNEEQKKLIGYIRDESNRLLRLTGELLDISQVETGKIVLDIRSARPAEIIGYAVK
                     AIEPQARQKNVTIKSVLPESVPDVLADTEKAAWVLINLLSNAIRYAPENSSVEINATR
                     NDGTVRFSVRDQGRGIPEEYRERIFEKFFRAPGIKTEGTGLGLAIAKEFILSQKGNIG
                     FDSFPGKGTEFWFTLPTQ"
     gene            4464..6764
                     /locus_tag="VFU15_08130"
     CDS             4464..6764
                     /locus_tag="VFU15_08130"
                     /inference="COORDINATES: protein motif:HMM:NF019284.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717787.1"
                     /translation="MTRFLSALFCLSFLPLCAQEDSCNCSHVMQDSWNMFLSRYDVYE
                     TGKPYTGICTGYSKDGLLLKRMELKDGYVVSMQFNYVSGKKFYVYDGTKHDSICGHLL
                     HYQDVTGFLSDEHWIYYSHGKKKEKVIEYFMFGAQDQVGTKHSEYYEIYPAKDSIALY
                     TSYLSCKSGYLQGTAVTPDGFYDKAVIDGYYREFSGGNPSVTVLEGNYFRGCRTGKWI
                     TRYDNGQLKAEGNYVNFNYDGEWKTWYANGQLSSHYSYVNGNLDGHGMEWYENGQLKL
                     DAYYDGYYGAAVDSVYTEYYGNGQLKIKYEIKKFPAGKSVAFRYVGTYTTWFENGQKE
                     SERNYDTSGYNNGPETTWYPNGVVKSILTDSIGVQVGPEKEYYDTGVLKRVTSPPAPI
                     FPRLVIDYWPNGNIKSNVCYGCSGMKDSLCSYYYPNGKIRREESWKAGSKNGKWTYTD
                     SLGNVTRVVNYHDMEVNGHCYDYFPGGKLKRDFTYKDNLRDGKCREWNADGKLVYDKK
                     FSRGKMKTGRGKKILPVASDSLRNAYARDAKIIAYWEVNMQDSLYGDSITASRKFQAQ
                     IENELLLLNNYFSKDYPFLDTLHIDPATGRTWMEITFPYTGRRIPGDTMGQCRDLGWP
                     VALDSVIALYHLVPVPGECYSAGNYGNGNLMIRQFTSDLPLNQNRLDTLLHHLDAHCA
                     CSGVYPQSQDFYEPPKPVFRYGDRNFSVYTFCSLANGVPQDVKNYYSGYTGMLLQFTF
                     YVYPDGSIDLFNHDDDPQPWSPDYPE"
     gene            6784..>7495
                     /locus_tag="VFU15_08135"
     CDS             6784..>7495
                     /locus_tag="VFU15_08135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717788.1"
                     /translation="MRLALLLFFLLPASVVSAQKPDSCDCKSINSYAENHLGPGQYAK
                     DYSKTPYTGICGQLGYQGVPYLWHKYENGYLVKRIDYDYSSRTVTYFDDIRKDTLISV
                     QKTFTMDTKILVDEDIIYESGGNRYERDTKFWTNGDSLEVTRYRYLKRGEPGATDDWR
                     LAFNRDDYYWEPVKDGPYISFHEWGSAPTKFRHVVGNYTNGQRSGYWEVRFSNGNLKS
                     QGNFVNDRENGHWKYFQYY"
BASE COUNT         2006 a         1919 c         1905 g         1665 t
ORIGIN      
        1 gatcattgta ctggcgatca tcgttacact ttttctcatt cagcgcaaag gaacttcttt
       61 catcgggaat cttttcgggc cggtgatgct catctggttc acaatgatcg gtgtactcgg
      121 cttaggcgca ctgatgaaag atttttctgt tctgaaagcg ttgaatccct ggtgggcatg
      181 gagaatgttg tctgaacaca aaggaggatt ctggctgctc ggcggcgttt ttctctgcac
      241 aaccggggca gaagcattgt attccgatct tggccactgc ggcagaaaaa atatccagat
      301 cagctggctg tatgtgaaaa tctgtctcgt actctgttat ttcggtcagg ccgcgtggct
      361 gatgaacgca catgatgcaa cactcacacc ttttttcgga attgtaccgc actggttttt
      421 ctttccggca gtctccattg caacactcgc aacgatcatc gccagccagg cgctcatcag
      481 cgggtcgttc acgcttgttg ctgaagcgat acgactgaac gtgtggccga aaatgcgaat
      541 agtttttcct tccgatatca aagggcagct ttacattccg gcaatcaact ggttgctgat
      601 ggcgggttgc atcggtgtag tacttttttt caaagaatcg tcggaaatgg aagccgcttt
      661 cggattgtca gttacgttaa cgatgctgat gacaaccatt cttctcagtt tttatctcta
      721 cacgaaaaaa gtgaatcgcg ttgcggtgtt ttcactgctg ctcatttatc ttctcatcga
      781 aggatctttc cttgtcgcca acctggtgaa attccagcaa ggcggatgga tcaccgtgct
      841 gatcggttcg ctcatcttcg tgatcatgta cgtgtggttc cgtgcgggag aaatccgccg
      901 ctcattgcgg gaaacaacag atcttgaaaa attcatcccg gtattgaaag aattgagcag
      961 tgatatgact gttccgaaat acgcaacgaa tcttgtttat ctcacttctt cgtccgacga
     1021 aaataaagtg gaggaacgga tcattcattc tattctctac aaacaaccga agcgcgccga
     1081 tatttactgg ttcgttcata ttgaggtgct ggatgaaccg tatcacatgt cgtaccatgt
     1141 gaaagtttac gaacccgacg atattatccg cattgatttc aaactcggtt tcagggttca
     1201 gccacgcatc aacctgctct tccggaaagt ggtggaagaa atggttgaac ggaaagaagt
     1261 gaatatcaac agcaggtact gttcgcttga caagcacaac ataaccggcg atttccgttt
     1321 tgtcatcatg cagcgttttc tttcttttga gaatgaactt tccttccgga atgaattcat
     1381 tctcaagttg tatttcttcc tgaaaaagat cagtcttgcc gatgaaaaag aatacgggct
     1441 cgattatagc aacgtgacga tcgaaaaatc accgctcatt ctcagttcac ctaaaacaat
     1501 tccactcata cgcgataact gatggaacag gaagaagaaa ataaagagga gcagagtttc
     1561 atcgatctgc taaagaaatc gaaacgcggc aagctgaaaa tttacattgg catgagcgcc
     1621 ggtgtgggaa agacttaccg catgctgcag gatgcgcacg cgctcgtgaa gaatggtgtg
     1681 gatgtgaaga tcggctacat tgaaacccac aaccgcgcag agacgcatgc gctcacggaa
     1741 ggattgcccg tcattccccg gaagaaaata ttttacaaag ggaaagagct ggaggaaatg
     1801 gacctccagg agattctcaa cgagcggccg gaagttgtca ttgtcgacga gctcgcacac
     1861 accaatattc ccggcagccg gcatgaaaaa agatggcagg acgtgcttga tcttttagag
     1921 gccgacatca gcgtgatcac cgcggtcaat atccagcaca tcgaaacgat cagcgacgaa
     1981 gtaaaaaaga tcgtcggcat cgaggtgaag gaacgcgtgc ccgacagctt cctgcagatg
     2041 gccgacgaag tggtgaacat cgatcttacc gccgacgaac tgatcacgcg cctgaaagaa
     2101 ggaaagatct acgaccgttc gaaagtggaa caggccatga agaatttctt caggcctgaa
     2161 aatattcttc agctccgcga gctggcgctg aaagaagtgg ccgcacaggt agaacgcaaa
     2221 gtggaaacgg aagtgctcac cggtgaaaag ctgcggcatg aacgttttct cgcctgcatc
     2281 agttccaatg aagaaaaagc cagccgcatc atacgcaaga ccgccaggct cgccgcgtac
     2341 tacaattctt cgtggttttt gctttacgtg cagacgccgc acgaatcgta cgacaaaatc
     2401 ccgctggcga cgcaacgtca cctcatcaat aatttcaagc tcgcgacaga gctcggcgcg
     2461 gaggttatcc gcataaaagg cgaggatgtc gggcaggcca tcatggaagt ggccgaagaa
     2521 aaaaagatca ccaccgtctg catcggcgtt ccggaattca ggctgtggca gatcgttctt
     2581 aaaggcggat tattcaaccg ccttctcggg aaactgaaaa tttcggatat agatctgata
     2641 attttgtcgt aagatgaaag tgaccataaa agccaagctt cgtttcggac tgctgttcct
     2701 tttcgccgtg atactgacga tcggtgttct gggcgggctg ttcctttacc gtctatcgga
     2761 cgaatcggga gatatcataa aggataacta cgaaagcgtg gtttacacca aatccatgct
     2821 ggaagcctgc gacgttcttg ccgcttcccc cggcggcgaa gcggaacgca gggcgaaaga
     2881 gaaattctct gccagtctcc gcagccagat gaacaacatc acggaaaacg gcgaagataa
     2941 tcttacctcc cagctgggcg cagcttacag cgcatttgaa aaagatacgg gacgggcgaa
     3001 gctgaacgac ctctttacgc tccgcgggat actgtattcc atacaggacc tcaacctcca
     3061 tgcgatcacg cagaagaacc tgaggacgaa tgaaacttcc cacacggtca ttctctggat
     3121 gacgatcatc ggcaccatcg cagtgcttgt ttcgttttcc ttcgtcgtga attttcccgg
     3181 ctttattgcc aaccccgtac gcgaacttac cagcggtatc aaggagatcg ccgcccggaa
     3241 ttattcgaaa cggcttcact tcagctcgaa tgacgaattc ggcgagctcg cggccgcgtt
     3301 caacagcatg accgaaaaac tggaagagta cgaaagcagc aatctcgcaa aacttctctt
     3361 cgagaaaaaa aggctcgaca cggttatcag cagcatgcac gatgcgatca tcggcctcga
     3421 cgaaaacggg aatatacttt tcctcaatcc tgtagcggcg caactgctcg gcgtgaaaag
     3481 cgaaaacgtt accggaaaac aggcttcttc cgtcgcggct acgaacgacc tgctccggga
     3541 actgacccgg aacatcggaa aggaaagcaa tgaaaaagac ctgtctccgc tgaagatttt
     3601 cgccgacgga cgggaatctt tcttcaaccg cgaagtgctg cgggtaactg cggttcccac
     3661 cggggagagc gctccgcagg tgatcggtta cgtgatcgtg ctgaaaaaca tcacgcagtt
     3721 ccgtgagctc gacctggcga aaacgaattt catcgccacc gtttcgcacg aattgaaaac
     3781 gccgatttcc tcgatcaaaa tgagtctcaa cctcctcgaa gaccagcgcg tagggatgct
     3841 gaacgaggaa cagaaaaaac tgatcggcta tattcgcgac gaaagcaaca ggctgctgcg
     3901 tctcaccggt gaactcctcg acatatcgca ggtggaaacc gggaagatcg tcctcgacat
     3961 ccgctctgcg agacctgcag agatcatcgg ctacgcggtg aaagccatag aaccgcaggc
     4021 ccggcagaaa aacgtaacga taaaatccgt cctcccggaa tccgttcccg atgttctcgc
     4081 cgacacggaa aaagcggcct gggtacttat caacctgctc tccaacgcaa tccgctacgc
     4141 accggaaaat tcctccgttg aaataaacgc cacgcgcaat gacggcacgg tccgtttcag
     4201 cgtgcgcgac caggggcgcg gaatcccgga agaataccgc gaacgcattt ttgaaaaatt
     4261 cttccgcgcc ccgggcatca aaacggaagg cacggggctg ggtcttgcca ttgcgaagga
     4321 attcatcctc tcgcagaaag gaaacatcgg gttcgattca ttccccggga aaggaaccga
     4381 gttctggttc accctgccga cacaatagcg cggaactgct gtttcgcttc tgaacttttt
     4441 tcacattaac ttgtacagga aatatgaccc gtttcctgtc cgcacttttc tgcctttcct
     4501 ttctcccgct ctgcgcgcag gaagacagct gcaattgcag ccacgtcatg caggacagct
     4561 ggaacatgtt cctctcgcgt tatgacgttt acgaaacagg aaaaccgtac acgggcatct
     4621 gcaccggcta ttcgaaggac gggctcctgc tcaaacgcat ggagctgaaa gacggctacg
     4681 tcgtttccat gcagttcaat tacgtcagcg ggaaaaagtt ctacgtgtac gacggaacga
     4741 aacacgacag catctgcggg cacctgctgc attaccagga cgtgaccggt ttcctgtcgg
     4801 acgagcactg gatctattat tcacacggaa agaaaaaaga aaaagtgatc gaatatttta
     4861 tgttcggcgc gcaggaccag gtcggcacga aacattccga gtattacgag atctacccgg
     4921 cgaaagacag catcgcgctt tacacatcct atctgtcgtg caaaagcggc tacctgcagg
     4981 gaacggctgt aacgcccgac gggttttacg acaaggcggt gatcgacggt tattaccgtg
     5041 aattctccgg tggaaatcct tccgtgaccg tgctcgaagg aaattatttc cgcggctgca
     5101 ggacggggaa atggatcacg cgctatgata acgggcagtt gaaggccgaa gggaattacg
     5161 tcaactttaa ctacgacggg gaatggaaaa cctggtatgc gaacgggcag ctttcatccc
     5221 attattcgta tgtaaacggg aacctggacg ggcacgggat ggaatggtac gagaacgggc
     5281 aattgaagct ggacgcgtat tacgacgggt attacggagc ggcggtcgac agcgtttaca
     5341 ccgaatacta cgggaacggt caactgaaga taaaatacga gatcaaaaaa tttcccgccg
     5401 ggaaatccgt tgccttcagg tatgtcggaa cgtacaccac ctggtttgaa aacgggcaga
     5461 aagaatcgga gcggaactac gacacgagcg gttacaataa cggaccggaa acaacgtggt
     5521 acccgaacgg cgtggtgaaa tccattctca ccgattcgat aggagtgcag gttggcccgg
     5581 aaaaagaata ttacgacacc ggtgtgctga aaagagtgac gagtccgccg gctcccattt
     5641 ttccacgact ggtcattgat tactggccca acggaaacat aaaaagcaat gtctgctacg
     5701 gatgttcggg aatgaaggac agcctctgct cctactatta tcccaacggg aagatccgcc
     5761 gagaggaaag ctggaaggcg ggaagcaaaa acgggaaatg gacatacacc gattcgctcg
     5821 ggaatgttac gcgcgtcgtg aattaccacg atatggaagt gaacggacat tgctacgact
     5881 attttccggg cggaaagctg aaacgcgatt tcacgtataa ggacaatctg cgcgacggga
     5941 aatgcaggga atggaacgcg gacgggaaac ttgtttacga taaaaaattc agccgcggaa
     6001 aaatgaagac cgggagggga aaaaaaattc ttcccgttgc ttccgattca ttgcgcaacg
     6061 cgtacgcacg tgatgcaaag atcatcgctt actgggaagt caacatgcag gactcgcttt
     6121 acggcgacag catcaccgcg agccggaagt tccaggcaca gatagaaaat gaattgctcc
     6181 tgctgaacaa ttatttttca aaagactacc cgttcctcga cacgctgcat atcgatccgg
     6241 cgaccgggag aacatggatg gagatcacgt tcccctatac cgggcgccgc attcccggcg
     6301 acacgatggg gcaatgccgc gatctcggct ggccggttgc gctcgacagc gtcatcgcac
     6361 tttatcacct cgttcctgtt ccgggcgaat gctattccgc aggcaactac ggcaacggaa
     6421 acctgatgat ccgccagttc acgtccgatc ttccgctcaa ccagaaccgg ctcgacacgc
     6481 tcctgcatca cctcgatgct cattgcgcct gctccggcgt ttacccgcag tcccaggact
     6541 tttacgagcc acccaaaccg gtcttccgtt acggcgaccg gaatttcagc gtgtatacat
     6601 tctgcagcct ggcgaatggc gttccgcagg atgtgaagaa ttattattca ggatacaccg
     6661 gcatgctcct gcaattcact ttttacgttt accccgacgg ttcgatcgac ctgttcaacc
     6721 acgatgatga tccgcagccg tggagccctg attacccgga ataatttttg atgtaaaaaa
     6781 atcatgcgcc tggccctgct cttatttttt cttctgcccg cttccgtcgt ttctgcacaa
     6841 aagcccgaca gctgcgattg caaatcgatc aacagctacg cggaaaacca tctcggtccc
     6901 ggccagtatg cgaaagatta ttccaaaaca ccttacaccg gcatctgcgg tcagctcggt
     6961 taccagggcg tgccctacct ctggcacaaa tacgagaacg gctatctcgt caaacggatc
     7021 gattacgatt acagcagcag aaccgtaacg tatttcgatg atatccggaa agacacgctc
     7081 atttccgtcc agaaaacgtt cacgatggat acaaaaattc tcgtcgacga ggacatcatt
     7141 tacgaatcgg gcgggaaccg ttatgagcgc gacacgaaat tctggacgaa cggcgattcg
     7201 ctggaagtga cgcgttaccg ctacctgaaa cggggcgagc ccggcgccac cgacgactgg
     7261 agactggctt tcaaccgcga tgattattac tgggaacccg tgaaagatgg tccgtacatc
     7321 agcttccacg aatggggaag cgctcccacg aaattccgtc acgtcgtggg aaattatacg
     7381 aacgggcaaa gatcgggcta ctgggaagtg cggttttcca atggcaacct caaatcgcag
     7441 ggaaatttcg tcaatgaccg cgagaatgga cattggaaat attttcaata ctacg
//
LOCUS       DASTSF010000282         9499 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_622133,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000282 DASTSF010000000
VERSION     DASTSF010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 9499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9499
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_622133"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..806)
                     /locus_tag="VFU15_08140"
     CDS             complement(<1..806)
                     /locus_tag="VFU15_08140"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEU4717789.1"
                     /translation="MRSFFFTAIAFLFAAQLHAGNGNTYPQNYFRNPLDIPIQLAGNF
                     GELRPNHFHTGVDITTHGQEGLPVHAAADGYISRIKITPWGYGKAIYVTHPNGYTTVY
                     GHLSAYNGNIAAYVEKNQYINESFEIELFPSPGELPVKKGDVIAFSGNTGSSGGPHVH
                     FEIRDTKTEDAINPLLFGLPVKDDVAPVAVSLVVCPAEPGAVVNGQNGTYRIPLKASG
                     GGYVFANPKDSIVAYGRIGFAIEGYDKESVPKGKNGVYAVRLQMENKTICS"
     gene            901..2658
                     /locus_tag="VFU15_08145"
     CDS             901..2658
                     /locus_tag="VFU15_08145"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEU4717790.1"
                     /translation="MKFNIRAVSLCAAVLLLLSFSASSQDVSAKKRGKFTTHYSDGKK
                     ESSGKVRNYKKNGTWKYWDSEGRLEKIITWKDDRKNGAYMEYFHDGTVSVSGKYADEK
                     KDGIWKSFYEGGKPASVLVYTAGEYDGVQHWWYENGNNREISLYAGGTPVCKWTWYYN
                     GRPKSFEYYSNGLADGTWRVYPDPSQATDTFPQSIDNYSAGKKNGKHLGYAHGLKTEE
                     WNYTDDLLDGDYLKWDYTGNLGVKEHYVNGKKDGLCSYYNYGKLLHAENYKAGMLDGE
                     SVQYNSAGVVIYREWYREGEKDSAFSYFGNGKMATKRVYSYHIVQRMNDEWSVYDEWD
                     SAGAHLLHGTYHSETKTGEWTTFYPDGKIKSVTPYDNGTIKGTYKKWYSNGKPLIEME
                     CDGSNVVRPPKVWDSKGKPLKPGTKDYQELVDSSKPGEVYNDPTHYIPDRTKIPTDGL
                     IFVTPVVTDQGDVPPPDDVDVEGNFLQDTTGMIFTFAEQPASFPGGQDSLDAFLAKNL
                     RHPASCLGKSGKVFVQFVVEKDGTLSGITVARGIPGAPELSGEAVRVMMLSPKWNPAK
                     QNGREVRYKVTLPVTFRPE"
     gene            2669..3124
                     /locus_tag="VFU15_08150"
     CDS             2669..3124
                     /locus_tag="VFU15_08150"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEU4717791.1"
                     /translation="MKTSARIIVLFFASFLCAHAGAQVAPPPADTSAQAGVHSANAPD
                     TARNAYAEVMPQFPGGQSAFMLYLQQNIRYPADERKHHREGVVYVSFVVEKDGSITNV
                     RCVKGVNGAPGLSDEAVRVISAMPAWSPGSTNGKPVRVEVTQPVRFALH"
     gene            3321..3611
                     /locus_tag="VFU15_08155"
     CDS             3321..3611
                     /locus_tag="VFU15_08155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717792.1"
                     /translation="MKPLPEVIRSLKAKTEKMLALSEALKKENIRLASDIGRLKNRVE
                     ELEAANRLLEEKYKDLRIAGKVSGDTDRNLALKLRINELVREIDKVIAQLNR"
     gene            3608..3976
                     /locus_tag="VFU15_08160"
     CDS             3608..3976
                     /locus_tag="VFU15_08160"
                     /inference="COORDINATES: protein motif:HMM:NF017016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEU4717793.1"
                     /translation="MKDLDRNQKEPAAKAVNPKDKMGEKSVKITIAGRAYPLKVNASE
                     EAFVNDAARRINERLIQLESKYSVKDKQDLLAMTALQFATQYLEAQQKISDENTALLS
                     GLAAIETMLDAHISKPGAVS"
     gene            4099..5634
                     /gene="rny"
                     /locus_tag="VFU15_08165"
     CDS             4099..5634
                     /gene="rny"
                     /locus_tag="VFU15_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005790424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEU4717794.1"
                     /translation="MLIPILAGAGGLVLGIVITATILRRGVLRKAEDKLKKAEADGEA
                     LKKEKMLQAKEKFLQLKQEHEKAVAERNNQVQQSENRARQKEQTLNQKIEENRRKENE
                     LEKRRKDIEHQMELVHARQEEIDKLHRKQVEQLEAIAGISGEEAKAQLVESLKAEART
                     ESMAFVKEVMDEAKMTANKEAKRIVIQTIQRVATEHAVENSVSVFHIENDEMKGRIIG
                     REGRNIRTLEAATGIEIIVDDTPEAIILSGFDPVRREIARLALHQLVTDGRIHPARIE
                     EIVEKVKKQVEEEIVETGKRTCIDLGVHGLHPELIRMVGRMKYRSSYGQNLLQHSREV
                     ANLCSIMASELGLNAKMAKRAGLLHDIGKVPDDEPELPHAILGMKLAEKFKEKPEVCN
                     AIGAHHDEVEMTSLLSPIIQVCDAISGARPGARREIVEQYIKRLKDLESLALSYPGVD
                     KTYAIQAGRELRVIVASEKVTDKDAEKLAIDISQKIQNEMTYPGQVKVTVIRETRAVG
                     IAK"
     gene            5729..6997
                     /locus_tag="VFU15_08170"
     CDS             5729..6997
                     /locus_tag="VFU15_08170"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEU4717795.1"
                     /translation="MIRRYLSNDYTRNLATLVSATVVAQVVNVAFNWVLARYFYHPDQ
                     FGALSVFTSLVSFITIISCAKFDVALVAAKTEEDASRLFSLGFLVLFIVTALGFVAYG
                     LNLLPALHLYGGTAVFTWLWAAPVSAFLLTATQLMWMINVRDKKFRGISWVRIIETSA
                     NGFFSLVFFSLGTIGLLYGSLLSQLSSVLFLAWVVLKHNRLKSFFHPPAVLRDVARRF
                     ADFPRINILQGFFDIFQVSSVVIYIGKYFGEAAAGYYGQCMRILQIPMRLIILPVSHV
                     FFAEASEIFRNNGDLFSLVKRTAYRTALFAAPVPLVLLAVGPWLFAFVFGAQWKEAGV
                     YAQLLAPWIFFDLIRAPLVQVSSLLGKQRQVLLVTMAGNIILALLYVAGRHYSLSARW
                     MLAGISVSQSIIAVILVFLIFRMSKVFSTR"
     gene            6994..7869
                     /locus_tag="VFU15_08175"
     CDS             6994..7869
                     /locus_tag="VFU15_08175"
                     /inference="COORDINATES: protein motif:HMM:NF016602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717796.1"
                     /translation="MIYDCFTFFNELEVLEIRLNILDPVVDKFVLVEATKNHQGKDKP
                     LYFEENKERFAKFLPKIIHVVVRDYPPNPQNNTWVFEHHQRNCIADGLKQAKADDIIL
                     ISDLDEIPKPETVRAHKDEPGVKILRQKMFYYFINCVNESESAEGNYLWNGTVMIHFS
                     DIRNGKIQEFRELSMRLLAWFLKDWKRRAYWRFRLKRELKNAQRDVHFIDDGGWHFSY
                     LGGVDRIIKKLESFAHDEYNRDEFKDPKKIEEAINNGRDILGRDFRYRFVKIDDSYPE
                     WLLQNLERFPHLVKK"
     gene            complement(7871..9022)
                     /locus_tag="VFU15_08180"
     CDS             complement(7871..9022)
                     /locus_tag="VFU15_08180"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEU4717797.1"
                     /translation="MKEPGTIAFLTAADPSDKRAWSGIHWYVSRALEKHIGKVEFLGP
                     YQPAFVYNSGRLASKVVRMLTGKNYDYSHSLRLAKAYGDHFNRKLREKNFSLIFSSAA
                     SSESAFIEKGLPMYYLADATAAALTGYYPYFSKLTRSSAKEILEVERRGISRAGHLIY
                     ASQWAADSAMNDFQAPPAKISVIPFGANIDEIPPADAVLSRKRGDTCRLLFLGAQWER
                     KGGPIAFETLKELNRMGIKTELTVCGCVPPAKVSHPALRIIPFLDKRDPVQMQQFSAM
                     MMATDFLLLPTRAECFGIVFCEASAYGVPSVSTLTGGVSGAIRENVNGILLPYEAAGK
                     EYAMKIAESWSRENSYSELCVSSRKLFETELNWDAWALKVKSLMQRSGD"
     gene            9159..>9499
                     /locus_tag="VFU15_08185"
     CDS             9159..>9499
                     /locus_tag="VFU15_08185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717798.1"
                     /translation="MIDRFFTPRRLGAFFSGTFLFLWLFPSLRLLLVGTHEMRSLFDI
                     AVTVFPDMLVVLLLAVTLFQLRKRIAEKKVTLTRLDLVFGAFVLANVVYGFVLAMMPK
                     YSLMSFRITYLP"
BASE COUNT         2363 a         2444 c         2577 g         2115 t
ORIGIN      
        1 gagcagatcg ttttgttttc catctggagc cggaccgcgt aaacgccgtt ctttcccttt
       61 ggaacagatt ccttgtcata tccttcgatc gcgaaaccga tcctgccgta ggcgacgatg
      121 ctgtctttcg ggtttgcaaa aacatagccg ccgcccgaag ctttcagcgg aatacggtac
      181 gttccgttct gcccgttcac cacggcgccc ggttcggccg ggcagaccac gaggctcacg
      241 gcgaccggtg ccacgtcgtc cttcacgggc agaccgaaca gcaacgggtt gatcgcatct
      301 tccgttttcg tatcgcgtat ctcgaaatga acgtgaggtc cgccggagct tcccgtattt
      361 cccgagaatg cgatgacgtc accttttttc accggcagct cgccagggga aggaaacagc
      421 tcgatctcga acgattcatt gatatactgg ttcttttcga cgtacgcagc aatatttccg
      481 ttgtatgcgc tcaggtgccc gtacacggtc gtatacccgt tcgggtgtgt gacgtagatc
      541 gcttttccgt agccccacgg cgtgatcttg atgcgggaaa tgtaaccgtc ggcggcagcg
      601 tgcaccggca ggccttcctg tccgtgcgtg gtgatgtcga cgccggtatg aaaatggttc
      661 gggcgcagct ctccgaaatt tcccgccagc tgtatcggga tgtcgagcgg gttgcggaaa
      721 taattctgcg ggtaggtgtt cccgttgccg gcatgcagct gtgcggcgaa aagaaaagca
      781 atcgcggtga agaaaaatga tctcatgaca agctaaattt acgctagtca aaaacggtaa
      841 aaccgggtaa atttgataag tgttaaccct ttgctgttaa ttgactgatc cgctgaaaaa
      901 atgaagttca atatccgtgc cgtttcgctg tgtgcagctg tcctgctgct gctttctttt
      961 tcagcctctt cacaggatgt gagtgcgaaa aaaagaggga agttcacgac gcattattcc
     1021 gacgggaaaa aggaatcgtc cggaaaggtg aggaattaca aaaagaacgg cacgtggaaa
     1081 tactgggaca gcgaaggccg ccttgaaaaa ataatcacgt ggaaagacga ccggaagaac
     1141 ggcgcgtaca tggaatattt ccatgacggg acagtttccg tttccggaaa atatgcggat
     1201 gaaaaaaaag atggtatatg gaaatcattt tacgaaggag ggaagccggc ttctgttctt
     1261 gtgtacacgg ccggtgaata cgacggcgtt cagcactggt ggtatgagaa cggaaacaac
     1321 cgtgaaattt ccttgtatgc cggcggtacg ccggtctgca aatggacgtg gtattacaac
     1381 ggaaggccga aatccttcga atattacagc aacgggcttg cagacggaac gtggagagtt
     1441 tacccggatc cttcgcaggc aacggatacg ttcccgcaga gcattgacaa ttattcagcg
     1501 gggaaaaaga acgggaagca tctgggctat gcgcacggac tgaaaacaga agaatggaat
     1561 tataccgatg atttgctcga cggcgattac ctgaaatggg attacaccgg gaacctcggc
     1621 gtgaaagaac attatgtcaa cgggaaaaaa gacgggttgt gctcatatta taattacggc
     1681 aagctccttc atgctgaaaa ttacaaagca ggcatgcttg acggagaatc cgtgcagtac
     1741 aacagcgccg gcgtggtcat ttaccgcgaa tggtaccggg aaggagaaaa agattccgca
     1801 ttctcgtatt tcggtaacgg gaaaatggcc acgaagcgcg tttattccta tcacattgtt
     1861 cagcgcatga acgatgaatg gtctgtttac gatgaatggg attcggcagg cgcgcacctg
     1921 ctccacggga cctatcattc ggaaaccaag acaggggagt ggacgacatt ctacccggac
     1981 ggtaaaataa aatccgtcac accttacgac aatggaacga taaaaggcac atacaaaaaa
     2041 tggtacagca acgggaagcc gcttattgaa atggaatgcg acgggagcaa tgtcgttcgt
     2101 ccgccgaaag tgtgggacag caaaggaaaa ccgttgaagc cgggaacgaa agattaccag
     2161 gagctcgtcg acagcagcaa gcccggtgaa gtgtacaacg atcccacgca ttacattcct
     2221 gaccgtacaa aaattcccac ggacgggctc attttcgtga cgccggtcgt aactgaccag
     2281 ggcgacgttc cgcctccgga tgatgtggac gtggaaggga attttctgca ggacacgacc
     2341 gggatgatct ttacgttcgc agagcagccg gcttcatttc ccggcggaca ggattcgctc
     2401 gatgcatttc tggccaaaaa cctcaggcac cctgcatcct gcctgggaaa aagcggaaaa
     2461 gtttttgttc agtttgttgt ggagaaagat ggaacgcttt ccggaattac cgttgcccgg
     2521 ggaattccgg gagctccgga actttccggt gaggccgtgc gtgtcatgat gctttccccg
     2581 aaatggaatc ccgcgaaaca gaacgggcgc gaggttcgtt ataaagtaac gcttccggtc
     2641 acgtttcgtc ccgaataaaa acaacaaaat gaaaacttct gccaggatca ttgttttatt
     2701 cttcgcatct tttctttgcg cacacgcagg cgcacaggtt gctccgccgc ctgcggatac
     2761 gtcagcgcag gctggtgttc attccgcgaa tgccccggat accgcccgga atgcgtatgc
     2821 ggaagtgatg ccgcaatttc ccggcggaca gagtgcattc atgctatatc tgcagcagaa
     2881 catacgttac ccggctgatg aacggaagca ccacagggag ggcgtcgtgt atgtttcgtt
     2941 cgtcgtggag aaggatggct cgatcacgaa cgtacgttgc gtcaagggag tgaacggggc
     3001 tcccgggctt tccgatgaag cagtccgtgt gatcagcgcc atgccggcat ggtcgcccgg
     3061 aagtacaaac ggcaaaccgg tgcgggtgga ggtaacacag cccgtgcgtt ttgcattgca
     3121 ctgagtgtgc tcccggatca gaaaaggcga taattcaacc ggacagtatt tgcaactgat
     3181 ttctttggcc gcgaaggcat gaaagaacaa aaaataccgg ctttgttccg gaagtgcgca
     3241 cagttaaatt ctttcataac gttaataatc agcaaaaaac gttatcttcg tgaaagctac
     3301 cctttaatcg gaaaagcttc atgaaacctc ttccggaagt catacggtcg ctgaaggcaa
     3361 agactgaaaa aatgttagct ttgtcggaag ccctcaaaaa ggaaaatatc cgtttagcat
     3421 cggatattgg tcgtttaaag aacagggtgg aagaactgga agctgcgaac cgcttgctcg
     3481 aagagaagta caaagatctt cgcattgcgg gcaaagtttc cggggacacg gacaggaatc
     3541 ttgctttaaa actcaggata aacgagctgg tgcgggaaat cgacaaagtg atcgcacagc
     3601 tcaacaggtg aaagacctgg atcgaaatca aaaggaacca gccgcgaaag cggtcaatcc
     3661 aaaagataaa atgggcgaaa agtccgtcaa aataacaatc gcaggccgtg cttacccgct
     3721 gaaggtaaat gccagtgaag aagcgttcgt gaatgatgcc gcaagacgca tcaacgaacg
     3781 gctgatacag ctggagtcga agtactcggt gaaggacaag caggacctgc tggcgatgac
     3841 ggcacttcaa ttcgcaaccc aatatctgga agcgcagcag aagatcagtg acgaaaatac
     3901 agccctgtta tcgggactgg cagccattga aaccatgctg gacgcgcaca tttcgaagcc
     3961 aggggccgtt tcctaacggt ttatttacat ttcactgttg acgatttccc gccacccgac
     4021 ggtttatact gttgcggacg cgggattttt gttttcaatt attaaccgaa taaaacgcca
     4081 atacgaacta tggatcaact gttaatcccg atcctcgccg gtgcaggcgg cctcgtcctc
     4141 ggcattgtca tcaccgccac gatcttgcga cgcggcgtgc tgcgcaaggc tgaagacaag
     4201 ctgaaaaagg ccgaagccga tggagaagca ctgaaaaagg agaagatgct ccaggcgaag
     4261 gagaaattcc tgcaactgaa gcaggaacat gaaaaagccg tggcggaacg gaacaaccag
     4321 gtgcagcaat ctgaaaaccg cgcccgccag aaagaacaaa cgctcaacca gaagatcgaa
     4381 gagaaccgca ggaaggaaaa cgagctggaa aaacgcagga aggacatcga gcaccagatg
     4441 gagctcgttc acgcccgcca ggaagagatc gacaagctgc accgcaagca ggtggagcag
     4501 ctcgaagcca ttgccggaat ttccggcgaa gaagccaaag cccagctcgt cgaatcgctg
     4561 aaagcggaag cacgcacgga atccatggct ttcgtcaagg aagtgatgga cgaggcgaaa
     4621 atgacggcga acaaagaagc gaagcgcatc gtcatccaga ccatccagcg cgtcgccacc
     4681 gaacatgccg tcgagaattc cgtttccgtt ttccatatcg agaacgacga aatgaaggga
     4741 cgcatcatcg gccgtgaagg acgaaacatc cgcacgctcg aagcggccac cggcatcgag
     4801 atcatcgtcg acgatacgcc ggaggccatc atcctttccg ggttcgatcc cgtgagaagg
     4861 gaaatcgcac gcctcgcgct gcaccagctc gtcaccgacg ggcgcattca cccggcgcgc
     4921 atcgaggaga tcgtggaaaa agtgaagaaa caggtggaag aggagatcgt cgaaaccggc
     4981 aagcgcacct gcatcgacct cggtgttcac ggacttcacc cggagctgat ccgcatggtc
     5041 ggccggatga aataccgttc ctcgtacgga caaaaccttc tgcagcactc gcgcgaagtg
     5101 gcgaaccttt gctccatcat ggcttccgag ctgggactga acgcgaagat ggccaaacgt
     5161 gcaggactgc ttcacgacat cggcaaagta cccgatgacg aacccgaatt gccgcacgca
     5221 attctcggca tgaagctcgc cgaaaaattc aaggagaagc ccgaagtgtg caacgccatc
     5281 ggcgctcacc acgacgaagt ggaaatgacc tcgctgcttt cgcccatcat ccaggtgtgc
     5341 gatgcgatat ccggtgcacg tcccggcgca cgccgcgaga tcgtcgagca gtacatcaag
     5401 cgtttgaaag acctcgaatc gctcgcgctt tcgtatcccg gcgtcgacaa aacgtacgcc
     5461 atccaggccg gccgtgagct gcgcgtgatc gtcgcttctg aaaaagtgac ggacaaggat
     5521 gcggaaaaac ttgcgatcga tatttcgcag aagatccaga acgaaatgac gtatcccggg
     5581 caggtgaaag tcaccgtgat ccgcgaaacg agagccgtcg gaattgcgaa ataagaaaac
     5641 ctgttcatag caggaagtgc cggttcagcc ggcatttctt gtttcaggat aaaatgaaaa
     5701 tgtaattttg aacccacttc cctaagcggt gatcaggaga tacctcagca acgattacac
     5761 ccggaacctc gccacgctcg tttccgccac cgtcgttgcg caagttgtga atgtggcgtt
     5821 caactgggtg ctggcgcggt atttttatca ccccgaccag ttcggtgcgc tttccgtttt
     5881 cacttcgctt gtcagcttca tcacgatcat ctcctgcgcc aagttcgatg ttgcgcttgt
     5941 agcggcgaaa acagaagagg atgcttcgcg tcttttttcg cttggttttc tcgtgctgtt
     6001 catcgtcaca gctctgggtt tcgtggcgta cgggctcaat ttattgcccg cgcttcacct
     6061 gtacggcggc accgcggtct tcacgtggct ctgggcagcg cctgtttccg ctttcctgct
     6121 caccgcgacg cagctgatgt ggatgatcaa cgtccgtgat aaaaaattca ggggaatttc
     6181 atgggtgcgt attatcgaaa catctgcgaa cgggttcttt tcactcgtgt tcttttcgct
     6241 cgggacgatc gggcttttgt acggatcgct gctgagccag ctcagctcgg tgctcttcct
     6301 cgcctgggtg gtgctgaagc acaaccgcct gaaaagcttt tttcatccgc ccgcggtcct
     6361 gcgtgatgtg gcaaggcgtt ttgccgattt tccgcgcata aatatcctcc agggcttttt
     6421 tgatattttc caggtaagca gcgtcgtgat ctacattggg aaatatttcg gcgaagcagc
     6481 tgcaggttat tacgggcaat gcatgcggat actgcagatc ccgatgcgcc tgatcatcct
     6541 tcccgtttcg cacgtgtttt tcgctgaggc ttcggaaatt ttccgcaaca acggcgatct
     6601 tttttcgctt gtgaaacgta cggcataccg cacggccctg ttcgctgcgc cggttcccct
     6661 tgtgctgctt gccgtgggtc cgtggctgtt cgcatttgtt ttcggcgcgc aatggaagga
     6721 agccggcgtt tatgcgcaat tgctggctcc gtggatattt ttcgatctca tccgcgcccc
     6781 gctggtgcag gtgtcgtcat tgctgggaaa acagcggcag gtgctcctcg tgaccatggc
     6841 cgggaacatc attctcgccc ttttgtatgt tgcaggaagg cactactcgc tttccgcgcg
     6901 atggatgctg gccggcattt ccgtttcaca aagcatcatc gccgttatcc tcgtattttt
     6961 gatcttccgc atgtcgaaag ttttttccac cagatgatct acgactgttt tacattcttc
     7021 aatgaactgg aagtgctcga gatccggctg aacattctcg acccggtggt ggataaattc
     7081 gtcctcgtgg aagcgacgaa aaatcaccag ggaaaagaca agccgctgta tttcgaagag
     7141 aacaaggaac gtttcgcgaa attcctgccg aagatcatac acgtcgtcgt gcgcgattac
     7201 ccgcccaacc cgcagaacaa tacgtgggtc ttcgagcatc accagcggaa ctgcatcgcg
     7261 gacggactta aacaggcgaa agcggacgac atcattctca tttcggacct cgacgagatc
     7321 ccgaaaccgg aaaccgttcg ggcgcacaaa gacgagcccg gcgtgaaaat tctccgccag
     7381 aaaatgttct attacttcat caactgcgtg aacgaatcgg aatcggcgga aggaaattat
     7441 ttatggaacg gaacggtgat gatccatttc agcgacatac gcaacgggaa gatccaggag
     7501 ttccgtgagc tgagcatgcg cctgctcgcg tggttcctga aagactggaa gcgccgcgca
     7561 tactggcgtt tccgcctgaa gcgcgaactg aagaatgcgc agcgggacgt tcattttatc
     7621 gacgacggcg gatggcattt cagctacctc ggcggtgtgg atcgtattat aaaaaagctg
     7681 gaatcgttcg cgcacgatga atacaacagg gacgagttca aggatccgaa aaaaatcgag
     7741 gaagcgatca ataacggtcg tgacattctc ggccgcgatt tccgttaccg tttcgtgaag
     7801 atcgatgatt cttacccgga atggctgctg cagaacctcg aacggtttcc gcacctggtg
     7861 aagaaataga tcagtcgccg gaacgctgca tgagcgattt cactttcagc gcccatgcgt
     7921 cccagttgag ttcagtttca aaaagttttc gcgacgacac gcacaattcc gaataggaat
     7981 tttcccggct ccagctctct gcaattttca tcgcgtattc tttcccggcc gcttcatagg
     8041 gaagcaggat cccattcacg ttttcgcgta tcgccccgct tacgccgccg gtaagagttg
     8101 aaacggatgg tacgccatag gccgaagctt cgcagaaaac gatcccgaaa cattccgccc
     8161 gcgtgggaag caggaggaaa tcagtcgcca tcatcatcgc ggaaaactgc tgcatctgca
     8221 cagggtcgcg cttgtcgaga aaaggaatga tgcgcagcgc aggatgcgac accttcgccg
     8281 gcggaacgca tccgcagact gtcagctccg tttttattcc catccggttc agttctttca
     8341 gcgtttcgaa cgcgatggga ccgcctttgc gttcccactg cgcgccgagg aaaagaaggc
     8401 ggcacgtgtc accgcgcttc cgggaaagta cggcatctgc aggaggaatt tcatcgatgt
     8461 tcgcaccgaa gggaatgaca gaaattttag ccggcggcgc ctggaaatca ttcatcgcgg
     8521 aatccgccgc ccactgcgaa gcatagatga gatgaccggc gcgtgaaata cctcttcgtt
     8581 ccacctcgag aatctcttta gcggatgacc gcgtcagctt cgagaaatac gggtaataac
     8641 cggtcagcgc agctgcagtg gcgtcagcga ggtaatacat cggcaatcct ttttcaatga
     8701 acgccgattc ggaagatgct gcggaagaaa aaatcaggct gaaatttttt tcacggagct
     8761 tgcggttgaa atgatcgccg tatgctttgg ccaggcggag cgagtgcgaa taatcgtagt
     8821 ttttcccggt gagcatgcgg accaccttcg atgcgagccg cccggaattg taaacgaaag
     8881 caggctggta cggcccgaga aattccactt tcccgatgtg cttttccagc gcccgcgaaa
     8941 cgtaccagtg tattcccgac caggcgcgct tgtcggaagg atcggcagcc gtaagaaatg
     9001 cgatggtacc cggctctttc atggtgagtg atccaaacct acttattcgg catgaaactg
     9061 gttttcccgc ggaggtaaat tatcaacacc gcccggctta aactttcggt tcccgggatt
     9121 gggggaacac cgctaatccg gtaatttcgt ttcacgcaat gatcgacagg tttttcactc
     9181 cccgccgtct tggtgctttt ttcagcggga cgtttctgtt cctctggtta tttccctcgc
     9241 tccgtctcct gctcgtcggc acgcatgaaa tgcgctccct gttcgatata gcggtaaccg
     9301 tttttcccga tatgctcgtc gtgctgctgc tggccgttac gttgtttcag ctgagaaaac
     9361 gcatcgcgga aaaaaaagtg acgcttaccc gccttgatct cgttttcggc gcctttgttc
     9421 ttgcgaatgt cgtgtatggt ttcgtgctgg ccatgatgcc gaagtattcg ctcatgagct
     9481 ttcgcatcac gtatcttcc
//
LOCUS       DASTSF010000283         3563 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1848573,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000283 DASTSF010000000
VERSION     DASTSF010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3563
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1848573"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..487
                     /locus_tag="VFU15_08190"
     CDS             <1..487
                     /locus_tag="VFU15_08190"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEU4717799.1"
                     /translation="LSHLVKPRVILEIGTFTGYSALCLSEGLSPDGKIISIDINEEHL
                     LIARKYRGLAGLDDRIEFVTGNAAELIPAMKQEFDLVFIDADKENYPLYYDLVVPKVR
                     SGGLIIADNVLWSGKVADENENDGETESLRKFARLALADERTEQVMLTVRDGLLLLRK
                     K"
     gene            complement(494..1093)
                     /locus_tag="VFU15_08195"
     CDS             complement(494..1093)
                     /locus_tag="VFU15_08195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717800.1"
                     /translation="MSREKYTAYFFVLAMLAVSCSGFSHMHYRKMRKVPAAGISSSPV
                     KQDVRAVPVSSFLIPEGKKTKTPMTVSEVLPRKKNLPSAFPQKHFREKTSHPVKTVQQ
                     KIPVHVLKAGTVKRRGGGEGMGLLIALLLLWLGIVLLSLGILLVWFAIAAIAWLALIG
                     GILLVVAGLLLAYGSVLTIFTGDHYHPDPYSQKPKSKKG"
     gene            complement(1090..1785)
                     /gene="mtgA"
                     /locus_tag="VFU15_08200"
     CDS             complement(1090..1785)
                     /gene="mtgA"
                     /locus_tag="VFU15_08200"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008236513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HEU4717801.1"
                     /translation="MKKLLLFSRRAVIFFCTFSFLLVLVYRWLPVSFTPLMFFREATC
                     SAGVKHEWVPLEKISNQLQLAVVSTEDQNFLHHHGFDFGAIRTAMDEAEDGGRRRGGS
                     TISQQTAKNVFLWPSSSFVRKGFEAWFTVLIELTWSKRRIMEVYLNSVEFGKGIYGCE
                     AASEFFFRKHASALGQDEAARLAVVLPNPIRYNVAKPGAYMQKRKSWAISQMNNLGGK
                     LLYGDEQDVKSRK"
     gene            1874..3127
                     /locus_tag="VFU15_08205"
     CDS             1874..3127
                     /locus_tag="VFU15_08205"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU4717802.1"
                     /translation="MNTLKEKLLRILRMGFRADMSEQDKRRVWIANLFYFTGTFMLTA
                     CTLQTFIVDGKDLGLYVLCSALCFQLTLPALLLRKFRLAESVMVIVAVATIFFFDNLM
                     GADAGVFIYYLPFLLMIGFIADFRHIGYILFYVAVTISAIGTSEIYNHHVFYHYHDPV
                     KNLESFHFNIIAASVFTSVITFLILWQNYLQYKLVSDQVEERKKNEEAMKISLREKEI
                     LLAEVHHRVKNNLAVISSLLNLQLNLSDNEDTRRVLLESRNRVSSMALIHQKLYRSSN
                     VEKIEFRSYCEDLIMQIKYSYPEAERAAIAVKMDVERTDLSLTVSIPLGLVLNELLSN
                     CYKHAFRGRTRGEIMIRFYRDEPARKFVLWVSDNGNGLPPGFEAGTANSLGMTIISSL
                     CVQLDGQFSITARPGGGTAAVLKFS"
     gene            3205..>3563
                     /locus_tag="VFU15_08210"
     CDS             3205..>3563
                     /locus_tag="VFU15_08210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717803.1"
                     /translation="MKKLLFQVFLVLFSIALPGLQQTFAQASACPSVDAGLDQNVCSG
                     QCTNLTATVQGSVGTSAYAVTSIPYNPYPYSGGNSVLINIDDTWSPAINMPFCFEFFG
                     NTYNQIVIGSNAIVTFDL"
BASE COUNT          828 a          995 c          890 g          850 t
ORIGIN      
        1 gctgagccat ctcgtgaaac cccgggttat tctcgagatc ggcacgttca ccgggtacag
       61 cgcgctctgc ttatcggaag gactttcacc ggacggaaag atcatttcca tcgacatcaa
      121 cgaggaacac ctgctcatcg cacggaaata ccgcggcctt gccgggctcg acgacaggat
      181 tgaattcgtt acggggaacg cggcggagct cattccggca atgaaacagg aatttgatct
      241 cgttttcatc gacgcagaca aagagaatta cccgctttac tacgacctcg tcgtgccgaa
      301 agtgcggagc ggcgggctca tcatcgccga caacgtgctg tggagcggaa aagtagcgga
      361 tgaaaacgag aatgacgggg aaacggaatc gctgaggaaa ttcgcacgtc ttgcactggc
      421 cgacgaacgc acggagcagg tgatgcttac cgtccgcgac gggctcctgc tgctgcgaaa
      481 aaaataaccg gcgtcatccc tttttgcttt tcggcttctg cgaataagga tcggggtgat
      541 aatgatcgcc tgtgaaaata gtcagcacgc ttccgtatgc gagaagaagg ccggcgacta
      601 ccagcagtat cccgccgatc agcgcaagcc acgctattgc cgcgatggcg aaccagacca
      661 gaagtattcc cagcgagaga agaacgatgc caagccacag cagcagcagc gcgatcagca
      721 ggcccatccc ttctccgccg ccacggcgct ttactgttcc tgctttaaga acatgcaccg
      781 ggattttctg ctgaacagtt ttcacaggat gggatgtctt ctcgcgaaaa tgcttttgcg
      841 gaaatgccga cgggagattt tttttccggg gcaacacttc gctcaccgtc atcggcgttt
      901 tcgttttttt cccttcagga atcaaaaaag aggaaacggg aacagcccgg acgtcctgct
      961 tcaccggcga agatgaaatt cctgccgcgg gaactttcct cattttccgg taatgcatgt
     1021 gcgaaaaacc ggaacacgat accgcaagca tagccagcac aaaaaaatac gcggtgtatt
     1081 tttcccggct catttcctgg attttacatc ctgttcatcg ccgtaaagca attttcctcc
     1141 gagattgttc atctgcgaga tggcccagct ttttcgtttc tgcatataag cgcccggttt
     1201 cgccacgttg taacggatgg gattcggaag tacgacagcg aggcgggccg cttcgtcctg
     1261 cccgagtgca gacgcatgct ttctgaaaaa aaattccgac gccgcttcac atccgtaaat
     1321 gcctttgccg aactcgacgc tgttgaggta aacttccatg atgcgtcttt tgctccacgt
     1381 cagttcgatc agcacggtga accaggcttc aaaacctttt cgcacaaaag agctgctcgg
     1441 ccagaggaaa acattcttcg ccgtctgctg gctgatggtg ctcccgcctc ttcttcttcc
     1501 gccgtcctcc gcttcgtcca tagcggttct tatcgcgccg aagtcgaacc cgtgatgatg
     1561 caggaagttc tggtcttcgg tgctcacgac ggccagctgc aactggttgg agattttttc
     1621 cagcggaacc cattcgtgct tcacgccggc ggagcaggtc gcttcgcgga agaacatcag
     1681 cggcgtgaac gaaaccggca gccaccggta gacaagcaca aggaggaaac tgaacgtgca
     1741 gaaaaagatc actgccctgc gtgaaaaaag caacagcttt ttcatataat ttctcatgat
     1801 cagatcaaag ttagaacgga taaaaactac ttttgaatga tttcccccgt ttttccaaca
     1861 tttccatttt gatatgaaca ccctgaagga aaagctgctc cgtatcctcc gaatggggtt
     1921 tcgcgcggac atgagcgagc aggacaagcg ccgggtgtgg atcgcgaacc tcttttattt
     1981 cacgggaacg ttcatgctga cggcctgtac gctgcagaca ttcatcgtcg acggaaaaga
     2041 ccttggcctg tatgttctct gctcggccct ttgcttccag cttacgcttc ccgcgctgct
     2101 tctccgcaaa ttccggcttg cggaatccgt gatggtgatc gttgccgtgg cgacgatctt
     2161 tttttttgac aacctcatgg gcgccgacgc aggcgttttc atctattacc ttccgttcct
     2221 gctcatgatc ggcttcatcg ccgacttcag gcatatcggc tacattcttt tttatgtggc
     2281 ggtcacgatc tccgcgatcg gcacgagcga gatctataat caccatgttt tttaccacta
     2341 tcacgacccg gtgaaaaacc tcgagtcgtt tcacttcaac atcatcgcgg cgagcgtgtt
     2401 cacttcggtg atcacgttcc ttattctctg gcagaactac ctgcagtaca agctggtttc
     2461 cgaccaggtt gaggaacgaa agaaaaacga ggaagccatg aaaatttccc tgcgcgaaaa
     2521 agagatcctt ctcgccgaag tgcatcaccg cgtcaagaat aacctggcgg tgatctcgag
     2581 cctgctgaat cttcagctca atctttccga caacgaagac acgcgccgcg tgctgctcga
     2641 aagcaggaac cgcgtctctt ccatggcgct cattcaccag aagctctacc gctcatcgaa
     2701 cgtggaaaag atcgagttcc gttcctactg cgaagacctg atcatgcaga tcaaatattc
     2761 ataccctgaa gcggagcggg cagcgattgc cgtgaagatg gacgtggaac gcaccgatct
     2821 ttcgctgacg gtttccattc cgctcggcct cgttctgaat gagctgctga gcaattgcta
     2881 caagcacgct ttccgcggaa gaacccgcgg agagatcatg atccgttttt acagggacga
     2941 gccggcccgg aaattcgtgc tttgggtcag cgacaatggc aacggccttc ctcccggctt
     3001 cgaggccgga acggccaatt cactcgggat gacgatcatc tcatcgctgt gcgtccagct
     3061 ggacgggcag ttcagcatta cagcacggcc cggaggcgga acggcggctg tattgaaatt
     3121 cagctgaata ggccccgggt aagtgttttt ttctatattt aaggttggca aaccaatggc
     3181 tcacctttat tatcccgtat gatgatgaaa aaattactct tccaggtctt cctggtgctt
     3241 ttcagcattg cccttcccgg acttcagcaa accttcgcgc aggcatcagc ctgtccgtcg
     3301 gtcgatgcgg gccttgacca gaatgtatgt tccggtcagt gtacaaatct caccgccacg
     3361 gtccagggat ccgtcggaac cagcgcatac gccgtcacat ccattcccta caatccgtat
     3421 ccctactccg gcggaaattc cgttctgatc aacatcgatg atacatggtc gccggccatc
     3481 aacatgccat tctgctttga atttttcggc aacacttata accagatcgt catcggttcc
     3541 aatgccatcg ttacgttcga tct
//
LOCUS       DASTSF010000284         7426 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_672142,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000284 DASTSF010000000
VERSION     DASTSF010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 7426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7426
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_672142"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..641)
                     /locus_tag="VFU15_08215"
     CDS             complement(<1..641)
                     /locus_tag="VFU15_08215"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018477971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HEU4717804.1"
                     /translation="MNLPASHTETLRQFLGDEFPQWEKANAQPAPVSLRVNPLKWKKE
                     FPAEKVSWCATGFYLPERPSFTHDPLFHAGHYYVQEASSMSIETVFEKYFPERDNLTV
                     LDLCAAPGGKSTHIVSLLGENSLLVSNEVIRHRANILSENLAKWGKDNVVVTNADPSE
                     FSKLEGFFDVILVDAPCSGEGLFRRDPEAAKEWSPENMELCAARQKRILLDVWP"
     gene            complement(736..1233)
                     /gene="infC"
                     /locus_tag="VFU15_08220"
     CDS             complement(736..1233)
                     /gene="infC"
                     /locus_tag="VFU15_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018943135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEU4717805.1"
                     /translation="MNEKIRDVTQVRVVGEGIEPGVYAIQKALDIAREAGLDLVEIVP
                     NADPPVCRVVDYKKFLYEQKKKQKEIKAKAQKIVVKEIRFGPQTDDHDFNFKKNHAIK
                     FLEGGAKVKAYVMFRGRSIVFKEQGEILLLRFAQELEEFGKVEQLPQLEGKRMQMLIA
                     PKKKK"
     gene            complement(1366..3312)
                     /gene="thrS"
                     /locus_tag="VFU15_08225"
     CDS             complement(1366..3312)
                     /gene="thrS"
                     /locus_tag="VFU15_08225"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017730806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEU4717806.1"
                     /translation="MINITLPDGAVRQYNEGTTALEIAKSISEGLARNVLAAKVNGEV
                     WDATRPINADSSLQLLTWNDRDGKEALWHSSAHLMAEALEALYPGIKFGIGPPIENGF
                     YYDVDPGDRVISADDFPKIEKKMMELAHQKNAYLRREISKADAVAYFREKGDQYKLDL
                     LEDLKDGSITFYSQGNFTDLCRGPHIPDTGFIKAAKLLNIAGAYWRGDETKKQLTRIY
                     GITFPKQKELDEYLVMLEEAKKRDHRKLGRELELFTFSEKVGMGLPLWLPNGAALRER
                     LQSFLQKAQLKAGYLPVVTPHIGHKQLYVTSGHYEKYGKDSFQPIRTPQEGEEFFLKP
                     MNCPHHCEIYKSKPRSYKDLPIRFAEFGTVYRYEQHGELHGLTRVRGFTQDDAHLFCR
                     PDQVKEEFCKVIDLVLYVFRALDFTDYTAQISLRDKVDRSKYIGSEENWNKAEQAIIE
                     AAKEKGLNTVIEYGEAAFYGPKLDFMVRDAIGRKWQLGTIQVDYNLPERFELEYTGSD
                     NQKHRPVMIHRAPFGSMERFVAVLIEHCAGKFPLWLTPEQVAILPISDKYIDYANRVS
                     EQLKNADIRGYVDDRSEKIGKKIRDNELKKVPYMLIIGEKEEADGTVSVRKQGDGDKG
                     AMPVSDFADLLNMEVRQRLESINS"
     gene            3839..5653
                     /locus_tag="VFU15_08230"
     CDS             3839..5653
                     /locus_tag="VFU15_08230"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HEU4717807.1"
                     /translation="MKKGLLSIAALSISFFCASQTILQQEDFTTYDGTSATIPAGWTF
                     SYNGNYTTVASSGTSGPNSYKFGANNATISTPQFSGADSVQFWVKGLSTDAVSMLVCL
                     ESPDNVTWDTVAKVKPVPATGTTLHYHVSSSAQYTRFTYVKSAGNVAFDDYRLIRNNA
                     QANSPGNITVYFNHPVNTSVSNGVNAVYLNQSIDDTLIAYINRAKYSLDIAVYNYIQT
                     SAISNIATAINNAYARGVKIRWIYNGSSSNSGLSQLNANIHTLGSPTTSTYGIMHNKF
                     MIVDAHSSDPSDPLVWTGSCNWDEQQINSDANNVIIIQDNNLAAAYTTEFNEMWGDTG
                     LVPNVANSKFGPDKTDNTPHTFTIGSRTVELYFSPSDGTNNHILSAINSADNTLYFGV
                     YTFTDATDANAIKTKDLAGVYTAGIIDQYSTSYTPYSTLSPVMGNRLRVYTQSNSIYH
                     NKFLIADDCDSTSDPVVLTGSHNWTVSADTKNDENTLIIHSAVIANIYFQAFDQNFID
                     LGGTLSPPCVFTTGMQTPEANDRFNVYPDPARDQLNIEASAGAKILVTDETGRAVMQF
                     IAPGENAFAIDISSLVPGLYFVREETATGISTRKIIVQ"
     gene            5656..6876
                     /locus_tag="VFU15_08235"
     CDS             5656..6876
                     /locus_tag="VFU15_08235"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEU4717808.1"
                     /translation="MKLVFTLLFPACLCAQSPVKILFDATKAETAGNADWVIDADQHN
                     LGYSNGPAVAGQGNESNAQHIPTPAQSGITANTPETFWNGSLSNWGIDCVKQGYEVET
                     LPYNGTISYGNSSNAQDLSNYKVFIICEPNIVFSAAEKTALLNFVQNGGGLFIVSDHD
                     QSDRNGDNWDSPGIWNDLFTNNSVQNDPFGFSVDLQNFSQTTTNIANLPGDSILHGPM
                     GNVAEAQWANGTTFTINHTDNPTTKGVIYKTSSSTTGTTNVMCLYGRYGNGKFAALGD
                     SSPCDDGTGDPNDALYNGYTSDAAGNHQLLIMNMTIWLAETNAVTTSLNDPGPANTLS
                     VFPVPSSGIFYVDGAKDFPSVITVFNSAGQEVSTQVADHFPFAIDLRDENDGMYFISV
                     ASGGKRSVLKAVKE"
     gene            complement(6878..>7426)
                     /locus_tag="VFU15_08240"
     CDS             complement(6878..>7426)
                     /locus_tag="VFU15_08240"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HEU4717809.1"
                     /translation="AAAAGLDYTNYPLGYQKLTDRHNFPVSNTLEYSNLLGYHFTPAQ
                     SEMQREWGLNNVEVPVMPVLTGKENSYGLFNFLGNVSEIIDGNCFKGLNYETALDGST
                     FLLKRGDYQVVDSTAYGYDYKFTFRYKKPQAWLGFRCACKVLNIPDDRKMLKHAGKMS
                     HAARKIEHRQWKSCMKYETKNH"
BASE COUNT         1651 a         1964 c         1987 g         1824 t
ORIGIN      
        1 ggccaaacgt cgaggaggat ccttttctgg cgggctgcgc acagctccat gttttccggc
       61 gaccattcct tcgcggcttc cgggtcgcgg cggaacaatc cttcacccga gcacggcgca
      121 tcgacgagaa tgacgtcgaa aaaaccttcg agcttcgaaa attccgaagg atcggcgttc
      181 gtcaccacga cgttgtcttt tccccatttc gcgagatttt ccgaaaggat gttcgcacga
      241 tgacggatca cttcattgga aacgagcagg ctgttttcgc ccagcagcga aacgatgtgc
      301 gtcgattttc cgcccggcgc cgcgcagaga tcgagtacgg tgagattatc ccgttccggg
      361 aaatattttt cgaaaacggt ttcaatgctc atcgagctgg cttcctgcac gtaataatgt
      421 cccgcatgaa aaagcgggtc gtgcgtgaag gacgggcgtt ctggcaggta aaatcccgtt
      481 gcgcaccagg aaactttttc cgccgggaat tcttttttcc atttcagcgg gttcacgcgc
      541 aggcttaccg gcgcgggttg agcattcgcc ttttcccatt ggggaaattc gtcgccgagg
      601 aattgccgca atgtttccgt atgtgacgcg ggaaggttca aaaaggaggg attgaaaaat
      661 cccaaactcc attttgtttt ctggaatccg ggatttggaa ttttgtcttt gaaatttgcg
      721 gccgaagatc gcctgttatt ttttcttctt cggagcgatg agcatctgca tccgttttcc
      781 ttcgagctgt ggaagctgtt ccactttccc gaattcttcg agctcctgcg cgaaacggag
      841 aaggaggatc tcaccctgtt ctttgaagac gatcgaacgt ccgcggaaca tcacatacgc
      901 ttttaccttg gcgccgccct cgaggaactt gatcgcgtgg ttcttcttga aattgaaatc
      961 gtggtcgtcc gtctgcggac cgaaccggat ctctttcacg acgatcttct gcgctttcgc
     1021 cttgatctct ttctgcttct ttttctgttc gtacaggaac tttttgtaat cgacgacgcg
     1081 gcagaccggc ggatcggcat tggggacgat ctcgacgagg tcgagtcctg cttcacgcgc
     1141 gatgtcgagc gctttctgga tggcgtaaac gccgggttca atgccttcgc cgacaacgcg
     1201 gacctgcgtt acatccctga tcttttcatt gatgcggtga agttcttctt ttctcggggg
     1261 acgtccgccg cgcggaaaaa acgggcgtgg acgttggtaa aatggagctg cgatggtaac
     1321 ctccgttttt ttagtttgac ttatgtgaat tatcgttgaa caaaattaac tgttaatgct
     1381 ttcgagcctc tggcggacct ccatattcag caggtcggcg aaatcggaaa ccggcatggc
     1441 gcctttgtcg ccgtcgccct gcttccgcac ggaaaccgtt ccgtccgctt cttctttttc
     1501 cccgatgatg agcatgtagg gaactttctt cagctcattg tcgcggatct tcttgccgat
     1561 cttctcgctg cggtcgtcca cgtatcccct aatatcggca tttttcagct gttcgctgac
     1621 gcggttggcg tagtcgatgt acttgtcgct gatcgggagg atcgccacct gttccggcgt
     1681 aagccagagc gggaatttcc cggcgcaatg ttcgataagc acggccacga aacgttccat
     1741 ggaaccgaaa ggggcgcggt ggatcatcac cgggcggtgc ttctggttgt cggaaccggt
     1801 atactcgagc tcgaaacgtt cgggcaggtt gtaatccacc tgtatcgtgc cgagctgcca
     1861 tttcctgccg atcgcgtcgc gcaccatgaa gtcgagcttc ggtccgtaga aagcggcttc
     1921 gccgtattcg atcaccgtgt tcagcccttt ttctttcgcc gcttcgatga tcgcctgctc
     1981 ggctttgttc cagttctctt ccgagccgat gtatttgctg cggtcgactt tatcgcgcag
     2041 cgagatctgc gcggtgtaat cggtgaaatc gagcgcacgg aaaacgtaca gcacgagatc
     2101 gatcaccttg cagaattctt ccttcacctg gtcgggacgg cagaaaagat gcgcgtcgtc
     2161 ctgcgtgaac ccgcgcacgc gcgtgagacc gtgaagctcg ccgtgctgct cgtagcggta
     2221 caccgttccg aattccgcga agcggatggg aaggtctttg tacgaacgcg gtttgctttt
     2281 gtagatctcg cagtggtgcg ggcaattcat cggcttgagg aaaaactctt cgccttcctg
     2341 cggtgtgcgg atgggctgga aagaatcctt cccgtatttt tcatagtgac ccgaggtcac
     2401 gtaaagttgc ttgtgcccga tgtgcggcgt cacgacagga aggtaacccg ccttgagctg
     2461 cgctttctgc aggaaagact ggaggcgttc acgcagtgca gcgccgttcg gcagccagag
     2521 cggaagtccc attcccactt tttcggagaa cgtgaaaagt tcgagctcgc gtcccagctt
     2581 gcggtgatcg cgctttttcg cttcctcgag catgacgagg tattcatcca gctctttctg
     2641 tttcgggaaa gtgatgccgt aaatgcgtgt cagctgtttt ttcgtttcat cgccgcgcca
     2701 gtaggcgccc gcaatgttga gcagcttcgc cgccttgatg aagccggtat ccgggatatg
     2761 cggaccgcgg cagaggtcgg tgaaatttcc ctgggaataa aaagtgatgg aaccgtcttt
     2821 caggtcttcg agcaggtcga gtttgtactg gtcgcctttt tcgcggaaat acgccacggc
     2881 atccgctttg gaaatttccc tgcggaggta agcgtttttc tggtgcgcca gctccatcat
     2941 tttcttctcg atcttcggga aatcgtcagc ggagatcacg cggtcgccgg gatcgacatc
     3001 atagtaaaaa ccgttctcga tcggaggacc gatgccgaac ttgatgccgg gataaagcgc
     3061 ttcgagcgct tcggccataa gatgcgcgga cgaatgccag agagcttctt tgccgtcacg
     3121 gtcgttccac gtcagtaatt gcagggaaga atcggcgttg atcgggcgcg tcgcgtccca
     3181 cacttcaccg tttacttttg ccgcgagaac atttcttgca agtccttcgc tgatcgattt
     3241 cgctatttca agcgccgtgg tgccttcgtt gtactgtcgt accgcaccat cagggagagt
     3301 tatgtttatc atccggtttt ttaaacctgc gtacaaagca gggtttttgt tgtggatgcc
     3361 aaaggtagta aaatcgcagc acctgcgagg tgacgggcgc aaagcccaaa acaggttgag
     3421 accgtcgatg cggaacgaaa atgcctcgct gacctccgat acggccggtt attcaaaaat
     3481 gtgcgcgtac agagattccc tcgaaaaaat gtgggcgtac cgcgattcgc tgaagccggt
     3541 catttttgcg aaaacaggtg attttacgag gatgattttc attgggcgac gcatgtacgc
     3601 gacacgacct gctacttccc cggtcgcgaa gcctggtacg ttccggcaaa aggagaaaca
     3661 ggcaccggct gggccgccag cctcgacctg ttcgggattc cctgcgactg atcgcaaaaa
     3721 aacgctgcgg caggataaaa tcagatgacg gccttagcag cgatttggca ggagtgttct
     3781 tgttgctaac ttaggaggtg tttacgcccc ggtaatgctg cactgataat taattcacat
     3841 gaaaaagggg ctcctttcca ttgctgcgct gagcatttct tttttctgcg cttcgcagac
     3901 cattctccag caggaagatt tcaccacgta tgacggaaca tccgcgacta ttcccgcggg
     3961 atggacattt tcctacaacg gaaattacac gacggttgca agctccggca cgagcggacc
     4021 gaactcgtac aagttcgggg cgaacaacgc aacgatcagc acaccgcagt tttcgggcgc
     4081 tgattccgtg cagttctggg tgaaaggttt gtcaacggat gcggtcagca tgttggtttg
     4141 tcttgaaagc ccggacaacg ttacatggga taccgtcgcg aaagtgaaac cggttccggc
     4201 aacaggaacc acgcttcatt atcacgtcag cagcagcgcg caatacaccc ggttcaccta
     4261 tgtaaaatcg gcgggcaatg tggcgttcga cgattaccgt ttgatccgga acaatgcgca
     4321 ggcgaattct ccggggaata tcacagtgta tttcaatcat cccgtgaaca cgtcggtttc
     4381 caatggcgtg aacgcggttt atctcaacca gtcgatcgat gacacgctca tcgcgtacat
     4441 caaccgtgcg aagtattcac tcgatatcgc cgtttacaat tacatccaga cgtccgcgat
     4501 ctcgaacatt gccacggcga tcaataacgc gtacgcccgc ggtgtgaaga tccgctggat
     4561 ctacaatggc agctcgtcga attctggcct gtcgcagcta aatgcgaaca ttcacacgct
     4621 gggaagtccg acaaccagca cgtacgggat catgcacaac aagttcatga tcgttgatgc
     4681 gcattcttcc gatccgtccg atccgctcgt ctggaccggt tcctgcaact gggatgagca
     4741 gcagatcaat tccgacgcga acaatgtgat catcatccag gacaacaatc tcgctgccgc
     4801 atacacgaca gagttcaatg aaatgtgggg cgataccggt cttgttccga atgtggcgaa
     4861 ctcgaaattc ggtcctgaca aaaccgacaa cacgccgcac acgttcacga tcggcagcag
     4921 gaccgtcgag ctgtatttca gcccttccga cgggacgaac aaccatattc tttccgcgat
     4981 caattcggca gacaacacat tgtatttcgg cgtgtacaca tttaccgatg cgaccgacgc
     5041 gaacgcgatc aaaacgaaag atctcgccgg tgtttacacg gccgggatca tcgaccagta
     5101 cagcacttcc tatacgccct attccacgct gagccccgtg atgggaaaca ggctgcgcgt
     5161 gtacacgcag agcaattcca tttaccacaa caagtttctt atcgccgacg actgcgattc
     5221 cacgtcggac ccggtcgtgc tgaccggctc gcacaactgg acggtttctg cagatacgaa
     5281 gaacgacgag aacacattga tcattcacag cgcggtgatc gcgaatattt atttccaggc
     5341 gttcgaccag aatttcatcg atctcggcgg aacgctttcc ccgccctgtg ttttcacgac
     5401 aggaatgcaa acgcctgaag cgaatgatcg tttcaatgtt tatcccgatc cggcgcgcga
     5461 ccagttgaac atagaagcca gcgcaggtgc gaaaatcctg gtcacggatg aaacgggccg
     5521 ggctgtgatg cagttcatcg ctccgggtga aaatgctttt gcaatagata tttcttcgct
     5581 cgtgcccgga ctttattttg tccgtgaaga aacagcaacc gggatttcaa cccggaaaat
     5641 aatcgtgcaa tgaaaatgaa attagttttt acgctgctct ttcccgcgtg cctgtgcgcg
     5701 caatcgccgg tgaaaatcct tttcgacgcg acgaaagcgg aaaccgccgg caacgccgac
     5761 tgggtgatcg acgccgacca gcacaacctg ggttacagca acggtcctgc ggtggcgggg
     5821 cagggcaacg aatcgaacgc acagcacatt cccacgccgg cgcaatccgg gatcactgcg
     5881 aatacgccgg aaacattctg gaacggatcg ctttcgaact ggggcatcga ttgtgtgaag
     5941 cagggctacg aggtggaaac gcttccgtac aacggcacga tcagctacgg gaattcttcc
     6001 aatgcgcagg acctttcgaa ttacaaggtg ttcattatct gcgagccgaa tattgttttt
     6061 tctgctgctg aaaaaacggc gctgctgaat tttgtgcaga acggcggcgg acttttcatt
     6121 gtttccgatc acgaccagtc ggaccgtaac ggcgacaact gggattcacc gggaatatgg
     6181 aacgatctgt tcacgaataa ttcagtgcag aacgatccgt tcgggttcag cgtcgatctc
     6241 cagaattttt cgcagacaac aacgaacatc gcgaaccttc ccggcgattc catcctccac
     6301 ggtccgatgg gaaacgtggc ggaagcgcaa tgggcgaacg gaacgacgtt cacgatcaac
     6361 cacacggata atcccacgac gaaaggcgtg atttacaaaa catcttcatc gaccacggga
     6421 acgacgaacg tgatgtgtct gtacggccgc tacggtaacg ggaaattcgc tgcgcttggc
     6481 gacagctctc cctgcgacga cggcacggga gatccgaacg acgcattgta caacggttac
     6541 acgtcggatg ccgcgggcaa tcaccagctg ctgatcatga atatgacgat ctggcttgcg
     6601 gaaacgaatg cggtgacgac ttcgctgaac gatccgggcc cggcgaacac actttctgtt
     6661 ttcccggttc cttcttccgg aattttttac gtggacgggg caaaggattt tccttcggtg
     6721 ataacggttt tcaattcggc aggacaggaa gtgagcacgc aggtcgcgga tcattttcct
     6781 ttcgctatcg atcttcgcga tgagaacgac ggcatgtatt ttatttccgt cgcctccggc
     6841 gggaaaaggt cggtgctgaa agcggtgaag gaatagctca gtggtttttt gtttcgtatt
     6901 tcatgcagct tttccattga cggtgttcta ttttccgcgc agcgtgtgac atcttgcctg
     6961 cgtgcttcag cattttccga tcatcaggaa tattcagcac tttgcatgcg cagcggaaac
     7021 cgagccaggc ctggggtttt ttataacgga aagtgaattt gtaatcgtac ccgtaagccg
     7081 tgctgtccac gacctggtaa tcgccgcgtt tcagcaggaa agtgcttccg tcgagcgctg
     7141 tttcatagtt gagtcccttg aagcaatttc catcgattat ttccgaaaca ttaccgagaa
     7201 aattaaaaag accgtagctg ttttccttcc cggtcaaaac aggcatgaca ggaacttcca
     7261 cattgttcag tccccattcc cgctgcattt ccgattgtgc gggtgtgaaa tgataaccga
     7321 gaagatttga atattcaagc gtgttggaaa caggaaaatt gtggcggtcg gtaagtttct
     7381 ggtaaccgag cgggtaattt gtataatcga gccctgccgc tgctgc
//
LOCUS       DASTSF010000285        20100 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1864033,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000285 DASTSF010000000
VERSION     DASTSF010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 20100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20100
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1864033"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1391
                     /locus_tag="VFU15_08245"
     CDS             <1..1391
                     /locus_tag="VFU15_08245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEU4717810.1"
                     /translation="VGESSFVYANPRMAEIFGYEQDELIGMEASDLVKSDYRHILERN
                     KQRRDAGENFSAHYEARGIKKDGTVIWVEVFSNSTQYKGKRGIIGTVSEITERKKAQE
                     ELEKSEANLRSIFNNTEIGYILVDQDFRVVAFNEVMRKGYAVASGINLDMGANLIDMV
                     VPARKARAQEIFGYVKKEGKPYEYEISFGTGKEMLFYNVILVPVKNGDEVIGVCLSAY
                     DITKRKMMEAERTEITNDLLGRNKDLEQFAHIVSHNLRAPVANILGLMNIMKLPSLTE
                     KDRERVDAGLVESVTRLDTVVKDLNRILQVRHGADETYEEIGLSELVKDVKISVGNLI
                     TAGDVNIRTDFSEAPQLKARKSYMHSIFYNLVSNSIKYRRNGVPCEIAISSVRGNDNV
                     ALHFSDNGRGFDKEKAGDKIFGLYKRFHDDVEGKGVGLFMVKTQVEAMGGKISFDSKV
                     NEGTSFVIRFGV"
     gene            complement(1489..2895)
                     /locus_tag="VFU15_08250"
     CDS             complement(1489..2895)
                     /locus_tag="VFU15_08250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717811.1"
                     /translation="MKKIFALLLTAAVIVSCNNNPPVSASASAPDTFVFTVTQSTKLT
                     RLPALQSFVFARDSVTGCWLMFSGRTNGFHNFPAAADSSFPVAHANHFAYVYNPALDT
                     LYSMNVSNLPAPLNVQYTFTNLESFQQGNNLYVCGGYGSNNFTNDSNNFVTANIISRI
                     NVNAMVTSIIAQNTSGFAASVVFDPKPHPVVCSTGGELYRLPGGKFYLCVGHRYYHFY
                     NPNGLNFPLQQYLDSVHVFTLTETPTSISLDSSSISYISDNQPDATTQFRRRDLVVSP
                     QVLGNGDSIGIAIYAGVFTQAGNPFTNPIYIPGYNGAAYAIDTFNQQSNYYSAPHFQL
                     YDSTADRMYTTVFGGIGSNPSFAGDNAAFTMQIVTLNRDFKAHTTSAIYNPSEMPNFV
                     GAEGIFIRANTAPAYHGNGYDIADIRAIPANQGVLVGYIFGGIVSNAPESTTNSCNPT
                     APSNVVYEVRMYKSGATK"
     gene            3220..4806
                     /locus_tag="VFU15_08255"
     CDS             3220..4806
                     /locus_tag="VFU15_08255"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HEU4717812.1"
                     /translation="MKIILPALFIFCSVGAATLRAQTSFVRSYGIWNESFRNNFQQTS
                     DGGYILSIDAVPEMDGNHQVDQGYLVKMDPQGNTQWIKNYDKSNIFIKSSDGTSVCQA
                     PDGGYAIGTTSYYSYNSSMIYLIKTDVAGNPLWAKHYPGLGSSACFCIRPTADGGYVL
                     CGNTKDTMNVWSNTFIYLLKTDGAGVPQWGMTYTETISGSGGTAYTVSETADGGYVLG
                     GYSSSGGFILKTDGNGHVQWGLNSMQAGNDAINSIRQTSDGGYIGCGTLDGFSLSAAL
                     LIKLDPAGSVSWTKAMKVPSDMLDFGLSAREVTGGYALLCSTDNGVMLAQTDANGQLL
                     WQHGTRGYPNGQMQVSYLETTTDGGFAFDYLFDQGAHGGVAVIKTDNSGMISCNANPD
                     TLTEMSCSAPLPIAFTTVLADPFLNTPVSFTTKNLSESDCPLIHTETVTGDFSIPNVF
                     TPNNDGINDYFLVNYDGGKDYRIRIYDRWGVLMFTSTDSALPWDGKARDGKEATDGTY
                     YYIIELGEQEHYTGFLTLLR"
     gene            complement(4812..5717)
                     /locus_tag="VFU15_08260"
     CDS             complement(4812..5717)
                     /locus_tag="VFU15_08260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HEU4717813.1"
                     /translation="MQQNRLKKSRLLKIFATLLLVPAAFLCVSWGFWAHKHINWWAIQ
                     TLPEPLQSFYKLHGDSIRERAVAPDLRRRTDKSEGMRHYLDADHYGVSPFDSIPQSWD
                     AAVKKYSADTLYKYGIVPWQITNVLKRLTQAFKDGNTDSIVFLSADLGHYVADAHVPL
                     HTTKNYDGQLTGQKGIHALWEARIVEMDSMSYNFKPTKARYLKDPQAEAWQILRDSYT
                     CLDSVLTLDQQVKKEIPQDQWYTNANVDGRDQKTFSTQYAAAYNKKLNGMIARRMQAS
                     VEDVGSYWYTAWVDAGKPDLAKKKK"
     gene            6186..10427
                     /locus_tag="VFU15_08265"
     CDS             6186..10427
                     /locus_tag="VFU15_08265"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HEU4717814.1"
                     /translation="MTCEKKYVAAIFAAQLFCIPAFSLQECGTNASYKTNWTVNPFGN
                     CVFVQNAGQFDESIREETNDAVLFMADFGKIKAYFTSHSIIYRCDDFPAEKKQEEGED
                     EKEGPGKPVSSYFTAGWKNSSSSVRVDADNEVPSYYSYAGTDRNAIIAHAFSRITYHD
                     LYPGIDVEYVLPEKGGLKYSLVVHPGADLSVVDLHYSADGKISTDENGNAIIPCKMGT
                     FTDHAPSCYYDDGSEASGSFSIDKRDLTFSIPGRDASRTLIIDPWTTNPNIVNVGWGP
                     NTNSAYDVDYDNYGNVYAYGSYYPFKLVKLNSAGVIQWVFTDPAFFSSNYGDFAVNRT
                     SGTCYLVEGVGTGVGGGAKVIKVNSNGTQVSIFPGDPGLLEMWRTAYDPCDNQVVIAG
                     GGNTVLNQACMLDTNMTAITPVNVLGATNAFHDMSLLCTDPASNNCFMATNRSNVNSA
                     SFDNRLLKLPMPSLSPATFNTWDGFTLHEAYINGYVLGPVSIGNPNSFTNGLNGMAAG
                     TNWLYTYDGTQLNKINKNTGAIVTMIWPYTWTWFHQDTVYWSGLDADLCDNVYVGVKS
                     SICVYNSSLVQTQTIPLSDTVYDVKVGQNNLLYACGQGFVSCITVTGGNTTTFTMTHQ
                     DPPACSCTGTATAHFTMTCGDTSNLTYTWSTNPVQTGQTAVNLCPGTYTVTVSLASGC
                     SSLNFSDTVTIAAPAGTLSIASSQNNVSCHGGTNGSAAVNVTGGSTPYTYAWSPAGGN
                     NATASNLAAGTYSCLITDANGCSQVQAVTITEPAALSVTTNISNISCMGGNGSATVNV
                     SGGSPGYTYAWAPSGGNNSTATNLAQGNYTCTITDTNGCVLTQSVSITQPAALAASFT
                     PVNVTCNGGANGSASVSVNGGTPAYTYAWAPSGGTGASASNLPAGTYTCTVTDANGCT
                     LTVAAVITEPPAITTTVTTNNSLCGSGTGSASIACAGGTSPYSYSWSPSGGTNATATG
                     LSAGNYTCTITDNNGCSVNATALITQQSNIVLATSITGEACFGDSNGSISVTPSGGTG
                     PYVFSWSSGGASATENNLPPGNYTVTVTDQNGCVQSDTFFVTEPPPLIVNASSAQSTV
                     CTGQNTTVSASISGGSPSYTYSWSPGNLPFLSYTFAPTASGTYTMNVTDANGCSASDS
                     VSVNVSPAPVPQFAASDSSACEMLCVDFTNNTSGATAYTWYFGDGDSSNVFSPTHCYT
                     SPGNYNVTLYAANGGGCSASVVYSNLIHVYPSPHADFLATPATTTILQPVISFTDTSS
                     GAITWWWNFGDGIGSSFSQDPVYTYTNTGEYYVTLTVTNSYGCTDTAGAFVYINEEES
                     FYAPNTFTPNGDGINDVFTPKGTGIDEGNYHLMIFDRWGNLIWETREWGEGWDGRANG
                     GSGIAQIDTYVWKADVSFRVSGTKKQYIGHVNLVK"
     gene            complement(10443..11795)
                     /locus_tag="VFU15_08270"
     CDS             complement(10443..11795)
                     /locus_tag="VFU15_08270"
                     /inference="COORDINATES: protein motif:HMM:NF016013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF389 domain-containing protein"
                     /protein_id="HEU4717815.1"
                     /translation="MEPLKQTWRFLKWRFGLSHDKNEAEVIIDSISKGIEFRGVNVWV
                     LVFAVFLASIGLNVNSTAVIIGAMLISPLMGPIMGIGLGLGIFDFDLVKSAAKNLAIM
                     VVVSLTASSLYFLLSPLNQAQSELLSRTAPTIWDVLIALFGGLAGIVASASKEKGNVI
                     PGVAIATALMPPLCAAGFGLARGDLLFFSGAFYLFCINAVFISVSTFLVVRLLKLPAR
                     SYPDPRIGRRIRNGITVIVLVTILPSMYIAYLTVRKVVFEQRVAEFVRDEFRFDGALV
                     LAQRTDYENNSLDITLIGKRIPQKTLDELKGKRSHYSLADLDIHVHQGLGDTTVPLDV
                     DRIKSQITEDLLSRNEAMLSDKDKTIAALQDELSTYRKNIVPVKDIAEEMKTLNENLL
                     ELSIYNTVSFNVAKNESDTVMTALGKFKSRPSRKETERLTTWLKTRTHADTLRFIITN
                     "
     gene            complement(11795..13036)
                     /locus_tag="VFU15_08275"
     CDS             complement(11795..13036)
                     /locus_tag="VFU15_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020527610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HEU4717816.1"
                     /translation="MNLFILFSVLISLAAVFSYINVRSLQLPSGIALMLLGSALSVVL
                     LVAGRVSPAFTGYIRAQLSVIDFSEFLLGILLSFLLFAGSLHVNMQLLKESAKSVIAF
                     STLGVVVSTLLTGTVLYFLLKISGLETPFVLCLLFGSLISPTDPIAVMGILKKSGVPK
                     PVEIMITGESLFNDGIGVVIFATIMQSASGGAGVSFGSVAWLFAQEAAGGILTGLLIG
                     YAGYRLMKSIDHFQTEILISLAMVMGGYSLCHFIHVSGPLAMVVAGLVTGNKGVTEAM
                     SDITRDYLEKFWEVMDEILNAILFTLIGLELIVVSFRTEYMIIGTVIAVVLVFIRYIS
                     IWIPASLFFFREKLGRKTMKIMTWGGLRGGISVALALSVPENEHKDALVAFTFVVVLF
                     SIAVQGMTIERLVKKLNPEKI"
     gene            13193..14719
                     /locus_tag="VFU15_08280"
     CDS             13193..14719
                     /locus_tag="VFU15_08280"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU4717817.1"
                     /translation="MKKIRVRRLKISDYERVREMQLHCFPNMKPWSFEQFKSLITVFR
                     EGQIGVEYDGKLVASSSSHILNFDQYSETDPWSVLTQNGFITNHDPDGDTLYGIEIMV
                     DPEYRGMKLSRRLYEARQKLVRDRNLRKITVGGRIPNYYKYADKLSAAQYVEKVIDKK
                     IYDPVLTAQIANGFVLKQLLPDYLPNDKESRGYATFLEWINYGYVPDGQKRKRQQYVR
                     ACAVQYQMRMIKSFKEFAKHCEYFVDVASDYRCDIVLFPEMFTMQLLTFLPEKSPAAA
                     MRSLADFTVRYEELFTRLAIKYNINIIAGSHFVIENDNLHNVSYLFRRNGTIGKQYKL
                     HITPHERKWWGVKPGNSVEVFDTDIGKIAILICYDVEFPELARIAVARGAEIIFVPFN
                     TDEKRGYLRVRYCAQARAIENQVYMVIAGCVGNLPEVENMDIQYAQSAIFTPSDTEFH
                     REGIANEASPNNETLIYQDLDMNLLNRSREFGSVLTWNDRRSDLYKIVYFENGKRKEI
                     "
     gene            complement(14729..15271)
                     /locus_tag="VFU15_08285"
     CDS             complement(14729..15271)
                     /locus_tag="VFU15_08285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEU4717818.1"
                     /translation="MKTRRSVVWLFFLAATLAACHNMQEYESYGQKFLSGNNDRKKAG
                     TGSLIFRSHRHNVLRIKRTETNDEYGTVEGNVVPAGKGIMVTLSDSVSVFSAHANPVS
                     GHFQFRYVPEGKYTLKVYPADGDEPLVSLPGIDVVAGMVTDAGNILVYHPKGRSGWLP
                     GSWSGYLADGGAEISALPQP"
     gene            complement(15326..15856)
                     /locus_tag="VFU15_08290"
     CDS             complement(15326..15856)
                     /locus_tag="VFU15_08290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717819.1"
                     /translation="MKTGKLTTRKAVICFFFMIIFAGMNVLSAQSAGYNSGLGLRFGS
                     DVGITGKTTVGKGYLEGILGSGYRAFMITGLYERYIPAFHRDDFHWYFGGGGHVGFFD
                     RWYAYGYYDRWGYYHGPYLYQYGDPTIGIDGIFGLEYKFQQIPFAASFDLKPFINLYR
                     YDYGFMEGALSFRYVW"
     gene            16025..16717
                     /locus_tag="VFU15_08295"
     CDS             16025..16717
                     /locus_tag="VFU15_08295"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEU4717820.1"
                     /translation="MHCLIVDDDEMIRTDLEQRIRRMPGLRLIASCGDATSAAEVMMK
                     NVIHLILLDVQLPGMSGIQFLKMLDRKKPAVVLITSRKEYAAEAFDYDVTDFLVKPFS
                     EERFLKAVMRASKYVQSNPGTAQADHIFVRVNSVLEKISLSEILYVEALADYVQIMSA
                     KQRYVVHATMKSMESALPSSLFFRAHNSFIVRLDRISRIEDNTILIGDKVIPVSRAKI
                     KPLLERLNLLRD"
     gene            16805..17188
                     /locus_tag="VFU15_08300"
     CDS             16805..17188
                     /locus_tag="VFU15_08300"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HEU4717821.1"
                     /translation="MRRSTRPYFMDTKHINLAELRTLGHNDETFIRRMLQTCAEQSPG
                     LLDGLKKATEENDTGAAAACAHRLRPTFHYLGRSDLSELLRAIEDGTFRDIDEMKTIS
                     QRFLDGAGRALGEVSEVLRMMNASS"
     gene            17185..19287
                     /locus_tag="VFU15_08305"
     CDS             17185..19287
                     /locus_tag="VFU15_08305"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU4717822.1"
                     /translation="MKSSLTYKVVLGFLLVAGTLSFASFFMFRELRRISSSINDIGPP
                     DRRIMLWKSVLDQLDRAGTCAESWKISSDPAVLDELDSCRTFIGDSLAQLRRLNAKFP
                     EAHALADSLTLLSDAWDEYLGDRIALSDTNLPGQLVADNVLREMAMHEMQASRSDMNF
                     DTTTVQDTLHPGKKQNFWQRVFGKRQTVQAQDVPAKDTAAHVSLIETSRVRNAIVKGQ
                     NQENLQENTRLRREMALLKTGISLQERLRHVTSRYEKIIGREEKEKFDAATRTSVEGT
                     MSIIFWIAGVALCLMLLFVYLIRRDIIRNRKLQAELDTARRNAEMLARTKEEFMANMS
                     HEIRTPLNIISGFSEQLLKSPLNDLQRIQAESIQRSSDHLLGIVNDLLDYSKMQSGKF
                     EFEEIGFSTSDLTGDLRSAFSGNATQKGLSFSVSCSSDVPEVLIGDPVRIRQVLYNLI
                     SNSVKFTERGKIDVALAKQKDLVDNTEWLRATVSDTGVGISNEKIEAIFEAFTQADNS
                     ITRRFGGTGLGLSITRFLVEKMNGTIHVESTEGKGTVFTVLIPMRRGSPKDIPGNTQK
                     AMPGNFLGGKTILVCDDEPVNRLLAGHILSAHGAQVLEAGGGKEALSILDQTKTDVVL
                     MDLQMPQMNGNEATQLIRSSENASIASVRIIAVTGRANPGEKEHCMENGMDGYVNKPY
                     KEEQLLSEIARVLGKIPA"
     gene            complement(19293..>20100)
                     /locus_tag="VFU15_08310"
     CDS             complement(19293..>20100)
                     /locus_tag="VFU15_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEU4717823.1"
                     /translation="LKVKFGGNAIEFHDNNFFVSEKRTVEFARLVHGEKMTWWGEGRI
                     DTIDKYKDESLEIMREAGCRMIFFGAETGNDERLRAMDKGGTQTGEQILRFAERMKKF
                     DIVPEYSFVLGTPAESEEEALRQVEEDIAFIRQVKEVNPQTEIIIYVYSPVPVEGSAL
                     YEKVKSNGFRFPEKLEDWISPQWEKFDLRKNPLTPWLTPKIVDRIRNFETVINARYPT
                     ISDTRLSSFRRNVMKISGTWRYSAKFYSLPLELKLLQKLWKYRQPEKEGF"
BASE COUNT         4874 a         5461 c         5379 g         4386 t
ORIGIN      
        1 tcgtgggtga aagcagcttc gtttacgcca atccgcggat ggcagagatt ttcggctacg
       61 aacaggacga attgatcggc atggaagcga gcgacctcgt gaagagcgac taccggcaca
      121 ttcttgagcg gaacaagcag agaagggacg ccggtgagaa tttcagcgca cattacgaag
      181 cgcgcgggat aaaaaaagac ggaaccgtca tctgggtgga agtattttca aacagcacgc
      241 agtataaagg gaaacgcggc atcatcggaa cggtcagtga aataacggaa agaaaaaagg
      301 cgcaggaaga actggaaaaa tcggaagcaa acctccgctc gatattcaac aatacggaga
      361 tcgggtatat tctcgtggac caggatttca gggtggttgc tttcaacgag gttatgcgaa
      421 aagggtatgc ggttgcttcc gggataaacc tggacatggg tgccaatctc atagacatgg
      481 tggttcccgc gcggaaggcg cgtgcgcagg agatcttcgg gtacgtaaaa aaagaaggaa
      541 aaccgtacga atacgagatc agcttcggca ccgggaaaga aatgcttttc tacaacgtca
      601 ttctcgttcc cgtgaagaac ggcgacgaag tgatcggcgt ctgcctttcc gcttacgaca
      661 tcacgaaacg aaaaatgatg gaagcggagc ggaccgaaat cacgaacgat ctcctggggc
      721 gcaacaagga tctcgagcag ttcgcccaca tcgtttcgca caacctgcgc gccccggtgg
      781 cgaatattct cgggctgatg aacattatga agctcccctc gctgacggaa aaagacaggg
      841 agcgcgtcga tgccggcctc gtggaatcgg taacgcgtct cgataccgtg gtgaaagacc
      901 tgaacaggat actccaggtg aggcacggcg cagacgaaac ctacgaggag atcgggcttt
      961 cggagctggt gaaggacgtg aagatcagtg tgggcaacct catcaccgca ggcgatgtga
     1021 acatccgcac ggatttctcc gaagctccgc aactgaaggc gaggaaaagc tacatgcaca
     1081 gcattttcta caacctcgtc agcaacagca tcaagtaccg ccgcaacggc gtgccctgcg
     1141 agatcgccat cagcagcgtg cgcgggaatg ataacgtggc gcttcatttt tccgacaacg
     1201 ggcgggggtt cgacaaggaa aaagccggcg acaagatatt cgggctttac aaacgtttcc
     1261 acgacgatgt cgaaggaaaa ggagtaggac tcttcatggt gaaaacacag gtggaagcga
     1321 tgggcggaaa aataagtttc gacagcaagg tgaacgaagg aaccagcttc gtcatccgtt
     1381 tcggggtatg atcaccagcg atacgcaggc acaaaaaaag aaggcgtctc cggtggaaga
     1441 cgcctttttc ttttcattcg ggtgaaacgc cggctgtttc ctgcatcctt atttggtcgc
     1501 gccggatttg tacatccgca cttcatacac gacgttggaa ggtgccgtgg gattgcagga
     1561 attcgtcgtg gattccggtg cattggaaac gatgccgccg aaaatgtagc cgacgagcac
     1621 gccctggttc gcgggtatcg ccctgatgtc cgcgatgtcg taaccattac cgtggtaggc
     1681 cggcgctgtg ttcgcacgga tgaaaattcc ttccgcaccg acgaagttgg gcatctctga
     1741 cgggttgtag atggcggaag tggtatgcgc tttgaaatcg cggttgagcg taacgatctg
     1801 catcgtgaac gcggcgttgt cgcccgcgaa ggaaggattg ctgccgatgc cgccgaagac
     1861 cgtcgtgtac atgcggtccg cagtggaatc gtacaactgg aaatgcggcg ctgaataata
     1921 attcgactgc tggttgaaag tatcgatcgc atacgcggcg ccgttatatc ccgggatgta
     1981 gatcgggttg gtgaacgggt tcccggcctg cgtgaacaca cctgcgtaaa tcgcaatacc
     2041 gatggaatcg ccgttcccga gcacctgggg agaaacgaca agatcacggc ggcggaactg
     2101 cgttgtcgca tcgggctggt tgtcggaaat gtagctgatg gaagatgaat cgaggctgat
     2161 ggaggtcggt gtttccgtga gcgtgaatac gtgaacggaa tcaaggtact gctgcaaagg
     2221 gaagttcagc ccgttcgggt tgtaaaaatg ataataacgg tgcccgacgc agaggtaaaa
     2281 ttttccgccg ggaagacggt acagctctcc tcccgtcgaa cagacgaccg ggtgcggctt
     2341 cgggtcgaag acgacagacg ctgcaaaacc ggacgtgttc tgcgcgatga tgctcgtcac
     2401 catggcgttc acgttgatgc gcgatatgat attcgccgtt acgaaattgt tggaatcgtt
     2461 cgtgaaatta tttgatccat agccgccgca cacgtagagg ttatttccct gctggaaact
     2521 ttcgaggttg gtgaatgtgt actgcacgtt cagcggagcc ggaagattgc tgacgttcat
     2581 actgtagagc gtgtccagcg caggattgta gacgtaggcg aaatgattcg catgagcaac
     2641 cgggaaagag ctgtcggccg cagcggggaa attatggaag ccgttcgttc ttccgctgaa
     2701 catgagccag caacctgtta cggaatcgcg cgcaaacacg aaagattgca gtgcaggaag
     2761 cctggtcagc tttgtggact gcgtcacggt gaacacgaac gtgtcgggcg cggaagcgct
     2821 ggcggaaacc gggggattgt tgttacagga aacgatgacc gcggcggtca gcaggagagc
     2881 aaagattttt ttcatgcggg aagattgatt tggttaaaat aaaagaacgc gggaacgcct
     2941 gacggaagtc ctcacgaacc tgtgaatgaa actgagaatt atgattaagg tgtaatgagc
     3001 ggttatggat aaaacaaaca tggcaaaaat cgggcgaaaa cacaagctga ttatttattc
     3061 acaaatagtt taagcggctg cccggatttt attcttccgg tcgacgggaa taaaaacgcg
     3121 cctcccttcc tgttttccgt ctatgccttt cgccgggcaa ctttttccgc ggaacattca
     3181 tcttcctgtt acgggttaat tggattaact ttaagtgaaa tgaagatcat ccttccggcg
     3241 ctttttattt tttgttccgt aggcgcagcg actctccggg cccagaccag cttcgtgcgt
     3301 tcctacggga tctggaatga atcgttccgg aataatttcc agcagaccag cgacggaggt
     3361 tacatcctca gcattgatgc cgtgccggaa atggacggga accaccaggt ggaccagggc
     3421 tatctcgtga aaatggatcc gcagggaaat acgcaatgga taaagaatta cgacaaatcg
     3481 aacattttca tcaaatcgtc tgacggaact tccgtgtgcc aggcgccgga cggcggttat
     3541 gcgataggta cgacaagcta ttattcgtac aacagctcga tgatttacct gatcaaaaca
     3601 gatgttgccg ggaatccgct ttgggcgaaa cattatcccg ggcttggcag cagcgcgtgt
     3661 ttctgcatca ggccgactgc tgatggcggc tatgtccttt gtggcaatac caaagacacg
     3721 atgaacgtgt ggtcgaatac attcatatac ctcctgaaaa ccgacggggc aggcgttccc
     3781 cagtggggaa tgacatacac ggaaaccatt tcaggatcgg gcggaacagc ttacacggtc
     3841 agtgaaacag cggacggagg atatgttctt ggtggatatt cctcatcggg cggcttcatc
     3901 ctgaagacag acggtaacgg ccatgtccag tggggactga attccatgca ggcaggaaac
     3961 gatgcgatca actccatccg gcagacgtcg gacgggggat atatcggctg cggcacactg
     4021 gacgggttca gtctttcagc agcattattg ataaagctgg atcccgccgg aagtgtttcc
     4081 tggacaaaag cgatgaaggt tccttccgac atgctggatt tcggtttgtc ggcgagagaa
     4141 gttaccggcg gttatgcttt gttgtgcagc acggacaacg gggtgatgct cgcacaaacg
     4201 gatgcgaacg ggcagctact ctggcaacac ggcacacggg gatacccgaa cggccagatg
     4261 caggtcagtt acctggagac gacaaccgac gggggctttg cgttcgacta cctttttgac
     4321 cagggagccc atggcggtgt cgcggtaatt aaaacggata attccggcat gatctcctgc
     4381 aatgcgaatc ccgatacgct caccgaaatg tcatgctctg cgcctcttcc catcgcgttc
     4441 accactgtac ttgctgatcc gtttctcaat acgccggttt ccttcactac caaaaacctt
     4501 tcggaatcgg attgtccgct tatacatacc gaaactgtaa ccggtgattt ttccatcccg
     4561 aatgtcttca ccccgaataa cgacggcatc aacgattatt tcctggttaa ctatgacgga
     4621 ggcaaagact acaggatccg catttacgat cgctggggcg tgctgatgtt cacgagcacc
     4681 gacagcgcat tgccatggga cggaaaagcg cgcgacggaa aagaagcgac cgacgggacg
     4741 tattattaca ttattgaact gggagagcag gaacactaca cgggtttcct gacgctgctg
     4801 cgctgaccgc tctacttttt ctttttcgcg aggtccggct ttcctgcatc cacccacgcc
     4861 gtgtaccagt agcttcccac gtcttccacc gaagcctgca tcctgcgcgc gatcatgccg
     4921 ttcagtttct tgttatatgc cgctgcatac tgcgttgaaa acgttttctg gtcgcggccg
     4981 tccacgttcg cattcgtgta ccactggtcc tgcggtattt cttttttcac ctgctgatcg
     5041 agcgtgagca cggaatcgag gcacgtgtag ctgtcgcgga gaatttgcca cgcttctgcc
     5101 tgcggatctt tcaggtaacg cgcttttgtc ggtttgaaat tatagctcat cgaatccatc
     5161 tccacgatgc gcgcttccca caacgcgtga attcctttct gtcccgtcag ctgcccgtcg
     5221 taattttttg tcgtgtgcag cggaacatgc gcgtcggcca cgtagtgacc gagatcggcg
     5281 gaaagaaaaa cgatgctgtc ggtgttgccg tctttgaacg cctgcgtgag gcgcttcagc
     5341 acattggtga tctgccaggg aacgatgccg tatttgtaca gcgtgtcggc agaatatttt
     5401 ttcacggccg catcccagct ctgcggaatg ctgtcgaagg gcgacacacc gtaatgatca
     5461 gcgtcgagat aatggcgcat gccttcggat ttgtcggtcc ggcggcgcag gtcgggagct
     5521 acggcgcgct cgcggatgga atcgccgtgc agcttgtaaa acgactgcag cggttcgggc
     5581 agcgtctgta ttgcccacca gttgatgtgc ttgtgcgccc agaatcccca gctcacgcag
     5641 aggaaagcgg cgggaacgag aaggagcgtt gcgaaaattt tcaggaggcg tgattttttc
     5701 agtctgttct gctgcatgtt tttccaggtg atggtgtgaa aaccggggcg cgtcatcagc
     5761 gtttattcaa aaaacgcgcc gttgttccgg gaccctaaaa ttagccggta aataaatgtt
     5821 tacataaggc ttttcgttat gccggtgtta ttttatgagg gtgtgaaacg gtggcatttc
     5881 gtctctgcac cagccccgga acagcgattt tttacatgaa aaacaagctt cagaactacc
     5941 gtttatttta tcatatacta tttactatca atatgataaa taaaatatgc aagactgaaa
     6001 ttaccacatt accaaaaaaa tggattgagc ctcctgccct gccgtgttaa gtttgatcgc
     6061 ctccaaaaac aacacgccat gaaacacgct tcggtttacc atcgcaccct ctcctggtcg
     6121 tcctgcctgc ggatttctct tttcatttcc atcctcgcca ccgtcacggt ttttatttcc
     6181 gctgcatgac gtgcgaaaaa aaatacgtcg ccgcgatctt tgctgcgcag ctgttttgca
     6241 ttcccgcttt ttccctgcag gaatgcggaa caaatgcatc gtacaaaacc aactggacgg
     6301 tgaacccgtt cggcaactgc gttttcgtgc agaacgcggg acaattcgac gaaagtattc
     6361 gtgaagaaac gaacgatgcg gttctcttca tggcggattt cggaaagatc aaagcgtact
     6421 tcacttcgca ttccatcatt taccgctgcg atgattttcc cgctgaaaaa aaacaggaag
     6481 aaggtgaaga tgaaaaagaa ggtccgggaa aaccggtctc ctcctacttc accgccggat
     6541 ggaaaaacag ctcttcttcc gttcgcgtgg atgcggataa cgaagttccc agttattatt
     6601 cctatgcagg aacagaccgc aatgcgatca tcgcgcatgc gttcagccgg atcacgtacc
     6661 acgatcttta tcccggcatc gacgtcgaat atgttctccc ggaaaaaggc ggactgaaat
     6721 attcgctcgt cgttcatccc ggcgccgatc tttctgttgt ggatcttcat tacagcgcag
     6781 acggaaaaat ttccacggat gagaacggta atgcgattat tccctgtaaa atgggaacgt
     6841 tcaccgacca cgctccttcc tgttattacg atgacggcag cgaagcgagc ggttcttttt
     6901 ccattgacaa acgcgacctc acgttcagca ttcccggccg tgatgcgtca cgcacgctca
     6961 tcatcgatcc gtggacgacg aacccgaaca tcgtaaacgt cggctgggga ccgaacacga
     7021 attccgctta cgacgtcgac tacgacaact acggaaatgt gtatgcgtac ggttcgtatt
     7081 acccgttcaa gctcgtgaag ctgaacagcg cgggagtgat ccagtgggtg ttcaccgatc
     7141 ccgctttttt ttcttccaac tacggcgact tcgccgtaaa cagaacttcc ggcacctgtt
     7201 acctcgtcga aggcgtggga accggcgtgg gcggcggcgc gaaagtgatc aaggtgaaca
     7261 gcaacggaac gcaggtgagc atttttcccg gcgatccggg tctgctggaa atgtggcgca
     7321 ccgcatacga tccgtgcgac aaccaggtgg tgatcgccgg cggcgggaac accgtgctca
     7381 accaggcctg catgctcgac acgaacatga ccgccatcac gcccgtgaac gtgctcggcg
     7441 cgacgaatgc atttcacgac atgagcctgc tctgcaccga tccggcgagc aacaattgct
     7501 tcatggccac caatcgttcc aatgtcaatt ccgcttcatt cgacaaccgc ctgctgaaac
     7561 tgcccatgcc ttcgctctca ccggcgacat ttaacacgtg ggacggattc acattgcatg
     7621 aagcttacat caacggttac gtgctcggac ctgtttccat cggcaacccg aacagtttca
     7681 cgaacgggct caacgggatg gcggccggaa cgaactggct gtacacgtac gacggaacgc
     7741 agctcaacaa aatcaacaag aacaccggcg ccatcgtcac gatgatctgg ccttacacgt
     7801 ggacgtggtt ccaccaggac acggtttact ggagcgggct cgacgcagat ctctgcgata
     7861 atgtttacgt cggtgtgaaa agttcgatct gcgtttataa ttcttcactc gtgcagacgc
     7921 agaccattcc gctcagcgat accgtttacg acgtgaaagt gggacagaac aatctgctct
     7981 acgcctgcgg gcagggattc gtttcctgca tcacggtgac cggcggaaac accacgacgt
     8041 tcacgatgac gcaccaggat ccgcccgcgt gcagctgcac cgggacagcc accgcgcatt
     8101 tcacgatgac ctgcggcgac acgtcgaacc tcacgtacac gtggagcacg aatcccgtgc
     8161 agacgggaca gacagccgtg aatctttgtc ccggaacgta cacggtaacc gtctcgcttg
     8221 cttccggctg cagcagcctg aatttttcag atacagttac gatcgcagcg ccggcgggaa
     8281 cgctgagcat cgcttcttcg cagaataacg tttcctgtca cggcggaacg aatggttctg
     8341 ctgccgtcaa cgtcaccggc ggaagcacgc cttacacata cgcgtggtcg cctgccggcg
     8401 gaaacaatgc gacggcgtcg aatctcgcag caggaaccta ctcgtgtctc atcaccgatg
     8461 cgaacggctg cagccaggtg caggccgtga cgatcaccga acccgccgcg ctttccgtca
     8521 cgacgaacat ctcgaacatt tcctgcatgg gcggcaacgg atccgcaacg gtgaacgtaa
     8581 gcggtggaag tcccggctac acgtacgcat gggcgccttc gggcgggaac aattccactg
     8641 caacgaatct cgcgcaggga aattacacct gcacgatcac cgataccaat ggttgtgttc
     8701 tgacacaatc tgtttccatc acgcaaccgg ctgcgctggc cgcttcattt acaccggtga
     8761 acgtgacgtg caatggcgga gcgaacggtt ctgcttccgt cagcgtgaac gggggcacac
     8821 ccgcctacac ctatgcgtgg gcgccttcgg gcggaacagg tgcgagcgcc tcgaatctcc
     8881 cggcgggaac atacacctgc acggtcacgg atgcgaacgg ctgcacgctc acggttgctg
     8941 cagtgatcac cgaaccgccg gcgatcacca cgaccgtgac gacgaacaat tccctgtgcg
     9001 gaagcggaac aggatccgca agtatcgcgt gcgcaggagg gacttcacct tattcttatt
     9061 catggtcgcc ctcgggcgga acgaacgcaa cagcaacggg gctttccgca ggaaattaca
     9121 cctgcaccat caccgacaac aacggctgct ctgtcaatgc gaccgccctc atcacgcagc
     9181 aatcgaacat cgtgctggca acgagcatca ccggtgaagc ctgtttcggc gacagcaacg
     9241 gatccatcag tgtcacgcca tccggcggaa ctggtccgta tgttttctcg tggtcgtcgg
     9301 gtggagcatc cgcaacggaa aacaatcttc cgccgggaaa ttacaccgtc actgtcaccg
     9361 accagaacgg ctgcgtgcag tcggatacat ttttcgtcac agagccgccg ccgcttatcg
     9421 tgaatgcatc ttccgcgcag tcaacagtgt gtaccggtca gaacaccacc gtttccgcga
     9481 gcatttccgg cggttctcct tcgtacacgt acagctggtc gccgggaaac ctgcccttcc
     9541 tttcttacac gttcgcaccg actgcatcag gaacatacac catgaacgtc acggatgcga
     9601 acggatgcag cgcgagcgac agcgtttccg ttaatgtaag tcctgcgccc gttccgcaat
     9661 tcgccgccag cgattcgtca gcatgcgaaa tgctctgcgt ggatttcacc aacaacacaa
     9721 gcggcgccac agcgtacacg tggtatttcg gcgacggaga ttcctcgaat gtattttcac
     9781 ccacgcattg ctacacatcg ccgggaaatt acaacgtcac actttatgcg gcgaatggcg
     9841 gaggctgcag cgcttcggtc gtttattcca atcttatcca cgtttacccg tcaccgcacg
     9901 cggattttct cgccacaccg gccacgacaa ccattctcca gccggtcatt tcattcacag
     9961 atacttcttc cggcgcaatc acttggtggt ggaatttcgg cgacgggatc ggcagcagct
    10021 tttcgcagga ccctgtttac acctacacga acacgggtga atattacgtc acgcttaccg
    10081 tgacgaattc ttatggctgc acggatacgg caggcgcttt cgtttacatc aacgaagaag
    10141 aatcgtttta cgcgcccaat acattcacgc cgaacgggga cggcatcaac gacgtcttca
    10201 ccccgaaagg aaccggcatc gacgaaggaa attatcacct gatgatcttc gaccgctggg
    10261 gaaacctcat ctgggaaacg cgcgaatggg gagaaggctg ggacgggcgt gccaacgggg
    10321 gaagcgggat cgcgcagatc gatacgtatg tgtggaaagc ggatgtgagc ttccgcgttt
    10381 caggaacgaa gaagcaatat atcggtcatg taaacctggt caaatgatcg caggagcagc
    10441 ggtcaattcg taatgatgaa ccgaagcgta tcggcgtgcg tgcgcgtttt cagccacgtg
    10501 gtgagacgtt cggtctcttt tcgtgaaggt ctgcttttga attttcccag cgcagtcatc
    10561 acggtgtcgc tttcgttctt cgccacgttg aacgaaaccg tgttgtaaat gctcagctcg
    10621 agcaggtttt cattcagcgt tttcatttct tccgcgatat ctttcacggg cacgatattt
    10681 ttccggtacg tgctgagctc atcctgcagc gcggcgatcg ttttgtcttt atcactgagc
    10741 atcgcttcat tgcgcgataa caggtcttcg gtgatctgcg atttgatcct gtcgacgtca
    10801 agcggaacgg tcgtatcacc gagtccctgg tgcacatgga tatccagatc cgcgaggctg
    10861 tagtgtgaac gttttccttt gagttcgtcc agcgttttct gcggaatacg tttcccgatg
    10921 agcgtgatgt caagcgaatt gttttcataa tccgttctct gcgccagcac gagcgcaccg
    10981 tcgaaacgga actcatcgcg cacgaattcc gcaacgcgct gctcgaaaac cactttccgc
    11041 acggtgaggt aagcgatgta catgctgggc aggatcgtga caagaacaat aacggtgatc
    11101 ccgttcctga tgcggcgccc gatgcgcgga tccgggtagg aacgtgcggg aagcttcagc
    11161 aggcgaacga cgaggaacgt cgacacgctg atgaagaccg cgttgatgca gaaaagatag
    11221 aacgcacccg aaaagaaaag aagatcaccg cgggcgagac cgaaacccgc cgcgcagagc
    11281 ggaggcatga gcgccgtcgc gatcgcaacg ccggggatca cgttcccttt ttccttcgaa
    11341 gctgaagcga cgatccccgc cagtccgccg aagagcgcga tgagcacgtc ccagatcgtg
    11401 ggcgccgtgc gcgacagcag ttccgactgc gcctggttga gcgggctcag gagaaaataa
    11461 agcgaggaag cggtgaggct caccaccacc atgatggcaa gatttttcgc tgcggatttt
    11521 acgagatcga aatcgaagat gccgagaccg agaccgatcc ccatgatcgg gcccatgagc
    11581 ggcgagatca gcatcgcacc gatgatgacc gcagttgaat taacgttcag ccctatggac
    11641 gcgaggaaaa cggcgaacac gaggacccac acattgacac cgcggaattc gatccctttg
    11701 cttatggaat cgatgatcac ttcggcttcg ttcttgtcgt gcgacaggcc gaaacgccat
    11761 ttcagaaaac gccatgtctg cttcagcggt tccatcagat cttttccggg ttaagtttct
    11821 tcaccagcct ttcgatcgtc attccctgta ctgcaatgga gaaaagcacc acgacgaacg
    11881 tgaatgcaac aagcgcatcc ttgtgctcgt tctcaggaac agaaagcgcg agcgcaaccg
    11941 aaatacctcc gcgcaatccg ccccaggtca tgatcttcat tgttttcctg ccgagctttt
    12001 cccggaaaaa gaaaaggctg gccgggatcc agatcgaaat ataacgtatg aaaaccagga
    12061 cgacagcgat cacggtcccg atgatcatgt attcggtgcg gaaactcacc acgatcagct
    12121 cgaggccgat aagcgtgaaa aggatcgcgt tcagtatctc atccatcact tcccagaatt
    12181 tttcgaggta atcgcgcgtg atgtcggaca tcgcttccgt cacacctttg ttcccggtga
    12241 cgagtccggc caccaccatg gccagcggac ccgaaacgtg aatgaaatga cagaggctgt
    12301 agccgcccat caccatggcg agcgaaatga gtatttccgt ctggaaatga tcgatcgatt
    12361 tcatcagccg gtaaccggca tagccgatca gcagcccggt gagaattccg ccggccgctt
    12421 cctgcgcgaa cagccaggcg acggaaccga agctcacacc tgcgccgccg gaagccgact
    12481 gcatgatcgt agcgaagatc acgacgccga taccgtcgtt gaacagcgat tcacccgtga
    12541 tcatgatctc caccggtttc ggcacgccgc tttttttcag gatgcccatc accgcgatag
    12601 gatcggtggg cgagatcagc gagccgaaaa gcaggcaaag cacgaagggt gtttccaggc
    12661 cggaaatttt cagcaggaaa taaagaaccg ttccggtgag cagcgtggaa acaacaacgc
    12721 cgagcgtgga aaacgcgatg acgctcttcg ccgattcttt cagcagctgc atgttcacgt
    12781 gcaatgaccc ggcgaagagg agaaagctca gcaatatgcc gaggaggaat tcggaaaaat
    12841 cgatcacgga aagctgcgcg cggatgtacc cggtgaatgc cggggaaacg cggccggcga
    12901 cgagcagcac gacgctcagc gcggaaccca gcagcatcag cgcgataccg gaaggaagct
    12961 gcagggaacg gacgttgatg taggagaaca ccgccgccag cgaaatgagc acggaaaata
    13021 aaatgaaaag attcacgccg gaaatattat cgcttcaaaa ttacgaattc atcaatgagg
    13081 aaaacctgat catgtcagcg cagcggcagg caggttattg attgcgcggg gagatttgat
    13141 taccttcagg aaaatccgct gtacattttt aaccaaccat gatttaccgc ttatgaaaaa
    13201 gatccgcgtg agaaggctga agatctccga ttacgaacgt gtgcgtgaaa tgcagcttca
    13261 ctgctttccg aacatgaaac cgtggagctt cgagcaattc aaaagtctca tcactgtttt
    13321 ccgcgaaggc cagatcggtg tggaatacga cgggaagctg gtcgcatctt cctcgagcca
    13381 catcctgaat ttcgaccagt attcggaaac cgatccgtgg agcgtgctta cgcagaacgg
    13441 gttcataacc aaccacgatc ctgacggcga cacactttac gggatcgaga tcatggtgga
    13501 cccggaatac cggggcatga aactttcgag gcgactttac gaagcgcggc agaaactggt
    13561 gcgtgaccgg aacctgcgga agattaccgt cggcgggcgc atacccaatt attacaaata
    13621 cgctgataag ctttccgcgg cgcaatacgt ggagaaggtc atcgacaaaa agatctacga
    13681 cccggtgctt accgcgcaga tcgcgaacgg gttcgtgctc aagcagctcc tgcccgacta
    13741 ccttcccaat gacaaggaat cgcgcggtta cgcgacgttc ctcgaatgga tcaactacgg
    13801 gtatgttccc gacgggcaga aaagaaaacg ccagcagtat gtacgcgcct gcgcggtgca
    13861 gtaccagatg cggatgatca aaagcttcaa ggaatttgcg aagcactgcg aatatttcgt
    13921 cgacgtcgct tcggattacc gttgcgacat cgtccttttc ccggagatgt tcacgatgca
    13981 gttgctgacg ttcctccccg aaaaatcacc cgcggccgcc atgcgttccc tggcagattt
    14041 cacggtccgc tatgaagaac tcttcacccg gctggcgatc aaatacaaca tcaacattat
    14101 cgccggatcg catttcgtga tcgagaacga caacctccac aacgtttctt acctgttccg
    14161 ccgcaacgga acgatcggga agcagtacaa gttgcacatt acgccgcacg aacgtaaatg
    14221 gtggggcgtg aaacccggca acagcgtcga ggtgttcgac acggatatcg ggaagatcgc
    14281 catcctcatc tgctacgatg tggaattccc ggagctcgcg cgcatcgcgg tggcgcgagg
    14341 cgcagaaatt attttcgtac cgttcaacac ggatgaaaag cgcggctacc tgcgcgtgcg
    14401 ttactgcgca caggcgcgtg cgatcgagaa ccaggtgtac atggtcattg cgggttgcgt
    14461 gggaaatctt ccggaagtgg aaaacatgga catacagtat gcacagtccg ccatcttcac
    14521 accttccgat acggaattcc accgggaagg aattgccaat gaagcttcgc cgaataatga
    14581 aacgcttatt taccaggatc tcgacatgaa cctgctcaac cgcagccgtg aattcggaag
    14641 cgtgctcaca tggaacgacc ggcgttcgga tctttacaag atcgtttatt ttgaaaacgg
    14701 gaaacggaag gagatctgag gaatttattt acggttgcgg gagcgcggaa atttccgcgc
    14761 ccccatcggc aaggtaacca gaccaagaac caggcaacca acctgagcgg cctttcgggt
    14821 gatagacgag gatgtttcct gcatccgtca ccatgcccgc gacgacatcg ataccgggta
    14881 acgaaaccag cggctcatct ccgtcagccg ggtagacctt cagggtatat tttccttcgg
    14941 gaacataacg gaactggaaa tgcccgctca cgggattggc gtgcgctgaa aaaaccgaaa
    15001 cactgtcgct cagcgtcacc atgatccctt ttcccgcggg aacaacattt ccttccaccg
    15061 ttccgtactc atcgtttgtt tccgtccgct taatgcgcag cacattgtgc ctgtgactgc
    15121 ggaagatcaa agaaccggtc cccgctttct tcctgtcatt gttcccggac aaaaactttt
    15181 gtccgtacga ttcatattcc tgcatgttgt ggcatgctgc cagtgttgct gccagaaaga
    15241 acagccacac gactgatctc cgggttttca tggcttttcg ttttacgttt catcctgtcc
    15301 atgtactctt tttcaaacgc gctcttcacc agacatagcg gaagctcaat gcgccttcca
    15361 tgaaaccgta atcgtagcgg tacaggttga tgaacggttt caggtcgaag ctcgcagcga
    15421 acgggatctg ctggaattta tattcaagtc cgaatattcc gtcgatgccg atcgtcgggt
    15481 cgccgtactg gtacaaataa ggtccgtggt aatagcccca gcggtcgtaa taaccataag
    15541 cgtaccagcg atcgaaaaat cctacgtgtc cgccgccgcc gaagtaccag tgaaaatcat
    15601 cacggtgaaa ggccggaata tatctttcgt acaatcccgt gatcatgaat gcgcggtatc
    15661 cgctgccgag aattccttcg aggtatcctt tgccgactgt cgtctttccg gtgataccga
    15721 catccgaacc gaaacgaagt cccaggccgc tgttgtatcc tgcggattgc gcagacaaaa
    15781 cattcattcc tgcgaagatg atcatgaaga aaaaacagat caccgctttc ctggttgtca
    15841 actttcctgt tttcatggct ctgctgtttt aattatgttt cattcactca aacaatttta
    15901 cttattcctc ttcccggccg ccgttttttt tcgacccttt gcccgacttg ctcgacagtt
    15961 tgaggccata ataaaggatg catttgcgtt atatttacaa aaccacccgc taattcaatt
    16021 tcctatgcat tgtcttattg ttgacgacga cgaaatgatc cgcaccgatc tcgagcagcg
    16081 catcaggcgc atgcccggtc tcaggctgat cgcttcctgc ggagatgcaa cttcggccgc
    16141 agaagtaatg atgaaaaatg tgatccacct catcctgctc gatgtgcagc tgcccggcat
    16201 gagcgggatc cagttcctga aaatgctgga ccggaaaaaa cccgccgtcg tgctgatcac
    16261 ttcgcgcaaa gaatatgctg ccgaagcgtt cgattatgac gtgactgatt ttcttgtcaa
    16321 gccgttttcg gaagaacgtt tcctgaaagc cgtgatgcgc gcttcgaaat acgtgcagtc
    16381 gaatcccggc accgcgcagg ccgatcacat tttcgtgcgc gtcaattccg tgctggaaaa
    16441 gatcagcctt tccgagatcc tctatgtaga ggcgcttgcc gattacgtgc agatcatgag
    16501 cgcgaaacaa cgctacgtcg ttcacgccac gatgaaatcg atggagagcg cgcttccctc
    16561 ttcgcttttt ttcagggcgc acaattcgtt catcgtccgc ctcgaccgca tttcacgcat
    16621 cgaggacaac accatactca tcggcgacaa agtgatcccg gtaagccgcg ccaaaataaa
    16681 accgctgctc gagcggctca acctgctccg cgactgaaac agggaatcga cgaacattgc
    16741 tcatcggtcg aaagtctcct gcgcagcaac gcaacccgct ttatttttgg atcgtgcgga
    16801 acgcatgcgc agatcaaccc gcccgtattt tatggacacg aagcacatta atctcgcaga
    16861 gctgaggacg ctggggcata atgacgaaac gttcatccgg cggatgctgc agacctgcgc
    16921 cgagcaatcg cccggtctcc ttgacgggct gaaaaaagca accgaagaaa acgacaccgg
    16981 cgcggcggcg gcgtgcgcac atcgcctccg tcccactttt cattacctcg gcaggagcga
    17041 tctttcagaa cttctgcggg cgatcgaaga cggaacattc cgcgacatcg atgaaatgaa
    17101 aacgatctca cagcgttttc ttgatggggc tggtcgcgcg ctcggcgaag tgtccgaagt
    17161 tctgcggatg atgaatgcct cctcatgaag agctcgctca cctataaagt cgttctcggt
    17221 ttcctgctgg tggcgggaac gctgagcttt gcctcttttt tcatgttccg cgagctccgc
    17281 cggatcagca gctcgatcaa cgacatcggt ccgcccgacc gcaggatcat gctctggaaa
    17341 tccgtgctcg accagctcga ccgcgccggc acgtgtgcgg aatcgtggaa gatcagcagc
    17401 gatcccgccg tgctggatga gctcgacagc tgccgcacct tcatcggcga ttcgcttgcg
    17461 cagctgcgca ggctcaatgc aaaattcccg gaagcgcatg cgctcgcgga cagcctcact
    17521 cttctttccg acgcgtggga tgaatacctc ggcgaccgca tcgctttgtc ggacaccaac
    17581 ctcccggggc agcttgttgc ggacaacgtg ctgcgcgaaa tggccatgca cgagatgcag
    17641 gcctcgcgat cagacatgaa ttttgatacg acgacagtgc aggacacgct gcatcccggg
    17701 aaaaaacaga atttctggca gcgtgtcttc ggcaagcggc aaacggtcca ggcccaggac
    17761 gtcccggcaa aagatacagc cgctcatgtt tcgctgatcg aaacgtcccg cgtgcgcaat
    17821 gcgatcgtca agggacaaaa ccaggaaaac ctgcaggaga atacccggct ccggcgggaa
    17881 atggcgctgc tgaaaacagg catctcgctc caggaacgcc tgcggcatgt cacgagccgt
    17941 tatgaaaaga tcatcggcag agaggaaaaa gaaaaattcg atgcggcaac ccggacctcc
    18001 gtcgaaggaa cgatgtcgat cattttctgg atcgccggcg tcgcgctttg ccttatgctt
    18061 cttttcgtat acctgatccg ccgcgacatc atccgcaaca ggaagctgca ggcggagctg
    18121 gatacggcgc gacggaacgc agaaatgctt gcgcgaacga aagaagaatt catggccaac
    18181 atgagccacg agatccgcac accgctcaac atcatttccg gtttttcaga acagctgctc
    18241 aagtcgccgc tgaacgatct gcagcgcatc caggccgaaa gcatacagcg ttcttcggat
    18301 cacctgctcg gcatcgtgaa cgacctgctc gactattcca agatgcaatc cgggaaattc
    18361 gagttcgagg agatcggttt ttcaacatcg gaccttaccg gcgatcttcg ctctgccttc
    18421 agcggcaacg ccacccagaa aggactttca ttttccgtca gctgcagcag cgatgtaccg
    18481 gaagtgctga tcggcgatcc cgtgcggatc cggcaggtgc tgtacaatct tatttccaat
    18541 tcggtcaaat tcaccgaacg gggaaagatc gatgttgcac tggcgaaaca gaaagatctc
    18601 gtggacaaca cggaatggct gcgcgccacc gtttccgata caggcgtcgg tatttccaat
    18661 gaaaagatcg aagcgatctt cgaagcattc acacaggccg acaacagcat cacgcgccgc
    18721 ttcggcggaa caggactcgg gctgagcatc acgcgtttcc ttgtcgaaaa aatgaacgga
    18781 acgattcacg tggaaagcac ggaaggaaaa ggaaccgttt tcactgtgct gatcccgatg
    18841 cgccgcggaa gcccgaaaga cattcccggc aacacccaaa aagcgatgcc cggaaatttc
    18901 ctcggcggaa aaaccattct cgtgtgcgac gatgaacccg tgaaccgcct tctcgccggt
    18961 cacatacttt ccgcccacgg tgcacaggtg ctcgaagccg gcggcgggaa agaagcgctg
    19021 tcgatcctgg accagacaaa aacagatgtc gtgctcatgg acctgcagat gccgcagatg
    19081 aacggcaacg aagcaacaca gcttatccgc tcttcggaaa atgcttcgat cgcttccgtg
    19141 cgcatcatcg ctgtaacagg acgcgcgaac ccgggtgaaa aagaacattg catggagaac
    19201 ggcatggacg gctacgtgaa caaaccgtat aaggaagagc aactgctcag cgagatcgcg
    19261 cgcgtgctcg ggaaaatccc cgcctgaaac attcagaaac cttctttctc cggctggcgg
    19321 tatttccaga gcttctggag taatttcagc tcgagcggaa gcgaatagaa tttcgcgctg
    19381 tatctccagg tcccggaaat tttcatcacg ttcctccgga aagaggaaag ccgcgtatcg
    19441 gagatcgtcg ggtagcgcgc gttgatcacg gtttcgaaat tacggatgcg gtccacgatc
    19501 ttcggcgtca gccagggcgt gagcggattt ttccgcaggt cgaatttctc ccattgagga
    19561 ctgatccagt cttccagctt ttccgggaaa cggaacccgt tcgattttac tttttcatac
    19621 agcgcggaac cttccacggg aacaggactg tatacataaa tgatgatctc cgtctgcgga
    19681 ttcacttcct tcacctgccg gataaaagcg atgtcctctt ccacctgcct gagcgcttcc
    19741 tcttccgatt ccgcaggcgt tcccagcacg aacgaatatt cgggaacgat atcgaacttc
    19801 ttcatccgtt ccgcgaaacg gagtatctgc tctcccgtct gcgttccgcc tttgtccatc
    19861 gcacgcagac gttcgtcatt cccggtttcc gcaccgaaaa agatcatcct gcagccagct
    19921 tcacgcataa tttccagcga ctcatctttg tatttgtcga tcgtgtcgat gcggccttct
    19981 ccccaccacg tcattttttc accgtgcacc agccgggcga attcgaccgt gcgtttttcc
    20041 gaaacgaaaa aattattgtc gtggaattcg atcgcgttgc ctccgaattt cactttcagg
//
LOCUS       DASTSF010000286        13799 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1175973,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000286 DASTSF010000000
VERSION     DASTSF010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 13799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13799
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1175973"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(124..1416)
                     /locus_tag="VFU15_08315"
     CDS             complement(124..1416)
                     /locus_tag="VFU15_08315"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEU4717824.1"
                     /translation="MTRKTSFSAHFKAKILLPFLLLLPFFVEAQGDMRFFGTATKDNK
                     PLPGATVTVLMDGKQIHQVTTGKNGKFKFTLDINHDYRINFSAPGCVDMYMTLDLHTP
                     PDKAWIYPDYVSDIPFFSAGDPKVKTELFAQKPFIKVIFDGGQGFKDDPTYRFIDEIF
                     KDPMEEQRKKEELARKQAADKARQDSIDLANKMANDALLAQQEADRRRKEEEDKSKNH
                     PANNPPADGNTNVPTMESDEIRLEKEKQEQLAREKQNKTIRAQYENNLLKLVAESEKK
                     TNISKFNKMKDEAMTNSVIEAMRREAEAKAQGTYLIEQQKEKQRQTLANRQIRAEEVS
                     RLIVTVAQIEKTLRVSGMKPVAASEMQNYAPLPNVVVTYEDGFFSDRTSTVFTWPGGK
                     RIIYNVIEFWWGGRYYYREGAEIDQKTYDAEIFRNKRS"
     gene            1546..2124
                     /locus_tag="VFU15_08320"
     CDS             1546..2124
                     /locus_tag="VFU15_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008611700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HEU4717825.1"
                     /translation="MNFNIKEIATATMVLFAVIDVVGSIPVIIGLKQKVGSLNSEKAS
                     LVSLAIMVGFLFVGEPILGLIGIDVSDFAIAGSFVLFFLALEMILGIRLYKDEVPSTA
                     SVMPLAFPLIAGTGTLTTLLSLRAEYSIISILIAIVLNVIIVYVVLRNISRLEKLLGV
                     GGVAVLRKVFGIVLLAIAVKLFRTHTHVWPGN"
     gene            2162..2695
                     /locus_tag="VFU15_08325"
     CDS             2162..2695
                     /locus_tag="VFU15_08325"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU4717826.1"
                     /translation="MLETKRLRLRKITPEDAPAIFGLRKNVRVHDFIYRPLQTKIEEA
                     EDIVKRVENGWLTRQSIGWAAEHKETGTLLAACGFNRIEAENLRAEIGGEMHPDFWGK
                     NFAQEGLGAIIRFGFETMNLHTVEARVISGNRSAIALLENNGFVQEGHLHEYGFFHGK
                     SFDLLIFALRNPGHRIV"
     gene            2826..4394
                     /locus_tag="VFU15_08330"
     CDS             2826..4394
                     /locus_tag="VFU15_08330"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEU4717827.1"
                     /translation="MPAYLWRLASRVNIYSRKQLWKWLLLVAAAIIVAASLIYTFHLE
                     HKISQSEKRNVELWAEAVRRKAIMMEETSQLYSKISKEERKKMEILANASRLLVNFDP
                     DRLDSKDTSFFEFIFRVIKSNETVPAIVTDGKGHIEQWRNLDTAKSRDPAWRYEQLKI
                     MRAQHDSIFYINDTLTKKTYYMFYRNSQMFDDIRESFESNERSFIKEVASNPASAPAI
                     YTDSTSSTIIAYGNLDTLKLHRDSLAYLHEALNYLKENGDTIMVSISEGRKNVIYYME
                     SPQLQQLRYFPIVQVIVVGMFLLVAYLLFSTSRRAEQNLVWVGMAKETAHQLGTPLSS
                     LIAWMEYLKMKGVDESILKEIGQDIRRLEMITERFSKIGSQPRLGNEDVVDALSHAVD
                     YMRSRTSKTVTFNYSPPASIFAPLNVPLFAWVIENLCRNAVDAMDGTGSITIEVFDEQ
                     QFVYIDITDTGKGIPRSKQKTIFEPGFTTKQRGWGLGLSLCKRIIEQYHKGEIFVKRS
                     LPGKGTTFRIVLKK"
     gene            4520..6847
                     /locus_tag="VFU15_08335"
     CDS             4520..6847
                     /locus_tag="VFU15_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013931317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU4717828.1"
                     /translation="MNFFLRRFFLFLLLLPAAAGAQNKYTVSGNVKNKKSGESIIGAI
                     IRIPEVKGAGAASNEYGFFSITVPEGDYTLTVSMTGYKPQSIPLSLHQNVHQDFMLED
                     TSFTQKEVVITGDRKDQNVTDPQMGAQKLDMKEVNKIPVLFGEKDVLKTIQLLPGIKS
                     GGDGGSGFFVRGGAADQNLILLDEAPVYNASHLLGFFSTFNSDAIKDVTVYKGNAPAQ
                     YGGRLASVLDVKMNDGNNQHYHVSGGLGLISSKLNVEGPIVKNKGSFLITGRRTYADL
                     FLKLSNNETTKNSQLYFYDLNLKANYELNDRNRIFLSGYFGRDKIGLANTFGIDWGNA
                     TGTFRWNHVTNERLFSNTSLIFSNYSYKISISGSTATFDITSKIRDYNLKHEYQFFPN
                     PKNSIRFGFNSIYHTVTPGQITAEAGSSINPVKLQDRYGWENAVFISNEWKATERISI
                     TYGLRGSSFSILGKGDFYSFDTEGNVSDTAHYSSGQIVKTYFNLEPRISGAVLINEKS
                     SVKFSYSRNTQYLHLLSNSTSTSPTDLWIMSSPIIRPELCDQYALGYYRNFRDNTIEC
                     SAEIYYKTMQHQIDYKNGANTQGNDLVEGELLFGIGRAYGLELFAKKNYGKFTGWVSY
                     TLSKSERQIAGINDGNWYPVKQDRTHDVSIVLMYDLTPKWSIAATWVYNTGDAVTFPS
                     GKYYVDGQVQFYYTERNGYRMPAYHRMDIGATWQRKKTDKFESSWNFSVYNVYARENA
                     YVITFRQDPNDASKTQAVQTSLFRLVPSVTYNFRF"
     gene            6867..7646
                     /locus_tag="VFU15_08340"
     CDS             6867..7646
                     /locus_tag="VFU15_08340"
                     /inference="COORDINATES: protein motif:HMM:NF025420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4249 domain-containing protein"
                     /protein_id="HEU4717829.1"
                     /translation="MKKIFFPLFAVFVLLASSCQKVIDVDLNSKDPQIVIEGNITDQP
                     GPYTVSITQTVNFSESNTFPPVTGATVNIADNVGNSETLTENSPGVYQTNSFQGVPGR
                     TYTLTVITNGKTYTAQSTMPSAIPLDSLLLEPNQSIGSPYYIIPLFQDPAGKGNYYRC
                     VETINHVRNNGIFLFDDQFSDGLINGEPILDFTTELAHNDSVDVELQCLDKATYTYFF
                     SLEQTLTGQTTAPANPVSNISNHALGYFSAHTVSRKSTVIP"
     gene            complement(7715..8140)
                     /locus_tag="VFU15_08345"
     CDS             complement(7715..8140)
                     /locus_tag="VFU15_08345"
                     /inference="COORDINATES: protein motif:HMM:NF020157.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1761 domain-containing protein"
                     /protein_id="HEU4717830.1"
                     /translation="MHFFSHANWLAIGVSVLVYYGLGALWFSVLFGKPWMAGHNIQMP
                     TDEAAKKEMRKKMPVMMLITLVMNIAMAMLIGFFVMALGAVNCMAGIKVGLALSAVAC
                     IPFMMSHLYLMKPFKVWVIDAGYHIVAITVMSIIISVWH"
     gene            8348..8875
                     /locus_tag="VFU15_08350"
     CDS             8348..8875
                     /locus_tag="VFU15_08350"
                     /inference="COORDINATES: protein motif:HMM:NF019614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HEU4717831.1"
                     /translation="MNKYIAFLRAVNVGGRVVKMDELKKIFSLPGFKNISTYIQSGNV
                     LFKTKETEVTVLKKKTENALKKALSYEVLVFLKTIPDIEKIIKRNPFKKEMEGMHLYV
                     SMLSEEPARENIPLLEKYRAPEERITIDGINAYILCPPKTYGTSKLSNSMLEKKLKVS
                     ATTRNWATMNKIIEL"
     gene            complement(9038..12010)
                     /gene="dnaE"
                     /locus_tag="VFU15_08355"
     CDS             complement(9038..12010)
                     /gene="dnaE"
                     /locus_tag="VFU15_08355"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014201709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEU4717832.1"
                     /translation="MLLNCHTFYSFCYGTLSVEEMLDEISSKGYRQFVVSDINNTSAV
                     LDTVRLSAARGLKPVAGIDFRNGVKQCYVGIARDNEGFRELNGHLSKHLHADEKFGPA
                     APEFRHAYVVYPANVYEGWPLRENEFIGVSLKDLQRIAFLPARRHPEKLVVLQPLTFI
                     HKRHHSAHRLLRAIDQNTLLSKLPAEEQATADEIIPHKNELLASYALYPSVVENTERL
                     LDDCRIEFEYGKLANKNLKHYTDSVTEDAELLRMECMHGLQYRYGEPSPEVLQRVEKE
                     LQVITQMNFASYFLINWDIVRYAQRKNYYYVGRGSGANSMVAYLLRITDVDPIELDLY
                     FERFINQFRSNAPDFDIDFSWTDRDDMTRYIFERFGHERTALLATYATFQHDAVIREL
                     GKVFGLPAGEIDRLQATHTHGDADELGRLVLRYSSFIKGFPSHLSIHSSGILISEKPM
                     TCYTATFMPPKGYPTTQFSMLEAEDIGLYKFDILSQRGLGKIKDALTIIEENRGAQID
                     IHDTRKFMQDEKVKQLLSVGKCIGCFYIESPAMRMLLAKLQADDYLRLVAASSIIRPG
                     VSKSGMMREYILRYRDKAKREKARAEIPELYDLLEETYGVMVYQEDVIKVAHFFAGLS
                     LAEADYLRRGMSWKFKQRNEFWRVKENFFRNCLAKGYAEKTITDTWNQIESFANYAFS
                     KGHSASYAVESFQALYLKAYFPLEYMVATLNNGGGFYRTEFYVHEARMHGAEIVQPCV
                     NNSGALCAIKGSVIYLGLGMISELEQNVINAIVNERERSGEFRSLYDFVKRVPVSVEQ
                     LRILVRANTFRFTGEHKRALMWEAHTLINPLKKNPHHTELFDVRPKEYRLPGLTSTWL
                     DDAFDDIGLFGFSLCSPFKLLRDKLPSPLTAKDLRHHIGKTVEIAGYLVTVKNTGTSK
                     GERMHFGTFLDTEGMWIDTVHFPPSAKAFPFRGPGCYLLKGKVVEEFDFTSIEVSEMY
                     RLPMLNVDDVREEELAGR"
     gene            complement(12041..13225)
                     /gene="dinB"
                     /locus_tag="VFU15_08360"
     CDS             complement(12041..13225)
                     /gene="dinB"
                     /locus_tag="VFU15_08360"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008990290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEU4717833.1"
                     /translation="MNRTVVHMDLDTFFVSVERLQDSSLIGKPVLIGGTSDRGVVASC
                     SYEAREFGIHSAMPMRTARMLCPHAIIKHGDHEQYSYYSNVVTDIIRESVPVYEKTSV
                     DEFYIDLTGTERFFGSYKLATDLRKTITRETGLPISFAMSANKTVSKVGTGEAKPNGQ
                     KEIPFGTEKHFLAPLSIRKIPMVGDKTYQLLRGMGVEKIKTVQEMPMELMTQVLGQNG
                     TALWKKANGIDNTPVEPYSERKSISTECTFEKDTIDVSYLRSVLISMTEKLAFQLRSE
                     QKLTSCVTVKIRYSDFNTYTMQMRIPYTSLDHMLIEKVKELFGKLYQKRMLIRLIGVR
                     FSHLVQGNYQFDLFEDTIEQLQLYQAMDRMRQRFGKNAVQRASGMGITHRDFNPFNGR
                     QT"
     gene            13303..>13799
                     /locus_tag="VFU15_08365"
     CDS             13303..>13799
                     /locus_tag="VFU15_08365"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU4717834.1"
                     /translation="MSRLATNLRILRKIKSSSQETVAEDLGIPRSRLGSYEEGRAEPP
                     CDMLLRISDYFHVSVDALLRGDFSKTDPESLMKIGKNRFLFPVLVDSKGNDYIEVVPV
                     KASAGYLNGYSDPEYVEKLPVMSLPFHVTGKHRSFPVKGDSMPPLKTGDFVIGKFVES
                     LNGIV"
BASE COUNT         3202 a         3844 c         3608 g         3145 t
ORIGIN      
        1 gtctgcacct gcgactgcac aagaccctgc tcgacgccgt aattgataaa gctcgaagtc
       61 tgtgcatgca gaaaaatgac tgacagcagg gccggcagta agaaaatgaa ttttgttttt
      121 ttcttaactc cgtttgttcc tgaaaatttc tgcgtcatac gttttttggt ctatttccgc
      181 tccttcgcga taataatatc ttccgcccca ccagaattca attacgttgt agataatccg
      241 ttttccgccg ggccaggtga aaacagtgct cgtgcggtcg gagaaaaatc cgtcctcgta
      301 cgtcaccacg acgttcggaa gcggtgcgta attctgcatt tccgaagcgg ccacaggctt
      361 catgcccgaa acgcgcagcg tcttttcgat ctgcgccacg gtgacgataa ggcggctcac
      421 ttcctccgcc cggatctgcc ggttggcgag cgtctgccgt tgtttttctt tctgctgctc
      481 gatcaggtag gttccctgcg ccttcgcttc agcctccctg cgcatggctt cgatgacgga
      541 gttcgtcatc gcctcgtctt tcatcttgtt gaatttcgag atgttcgttt ttttctccga
      601 ttcggcgacg agcttgagca ggttgttctc gtactgcgca cggatcgttt tattctgctt
      661 ttcacgcgca agctgttcct gtttttcttt ttcaagacgg atctcgtccg actccatcgt
      721 cggcacattc gtattaccgt ctgccggggg attattggca ggatggttct tgcttttatc
      781 ttcttcttcc ttccgcctgc ggtcggcttc ctgctgcgcg agcagcgcgt cattcgccat
      841 tttgttcgca agatcgatgg aatcctggcg cgctttgtct gctgcctgct tcctggccag
      901 ctcttccttc ttgcgctgct cttccatcgg gtccttgaaa atctcgtcga tgaagcggta
      961 cgtgggatca tctttgaatc cctgcccgcc gtcgaagatc actttgatga atggtttttg
     1021 cgcaaaaagc tccgtcttca ctttgggatc gccggcagaa aaaaacggaa tatcggaaac
     1081 gtaatccggg tagatccacg ctttgtcggg cggcgtatgc agatcgagcg tcatgtacat
     1141 gtcgacgcag cccggtgcgg aaaaattaat ccggtaatcg tggttgatgt cgagcgtgaa
     1201 cttgaacttc ccgttcttcc cggttgtcac ctggtggatc tgcttgccgt ccataagcac
     1261 ggtgaccgtc gctcctggca gcggcttgtt gtcttttgtc gctgtcccga aaaaacgcat
     1321 atcgccctgc gcttccacga aaaaaggcag caaaagcaaa aacggaagaa ggattttcgc
     1381 tttgaaatgc gcagaaaaag aagttttccg ggtcatgcgt tccagggcaa ttactaaggt
     1441 acgcaaaatg aaggagccgg gaacggtcgc ccggaaggcc gccggggacc gaaaacagcg
     1501 ttccgaacac gcaaaccttc tacttttgaa aaaaaaaatc ttccgatgaa tttcaacatc
     1561 aaagagatcg ccacggctac catggtgctt ttcgccgtga tcgacgtcgt gggatcgatc
     1621 ccggtcatca tcggtctgaa gcagaaagtg ggcagcctca actcggaaaa agcctcgctc
     1681 gtttcgctcg cgatcatggt cggttttctc ttcgtgggcg agcccattct cggcctgatc
     1741 ggcatcgacg tttccgattt cgccattgcc gggtcgttcg tgctgttttt cctggcgctg
     1801 gaaatgatcc tcggtatccg gctgtacaaa gacgaagttc cgtccaccgc atccgtcatg
     1861 ccgctggcgt ttccgctcat cgccggaacg gggacgctca ccacgctcct gtcgctgcgc
     1921 gcggaataca gcattatcag catcctcatc gccatcgtgc tgaacgtgat catcgtttac
     1981 gtcgtgctgc gcaatatttc ccggctggaa aagctgctcg gcgtgggcgg tgttgctgtt
     2041 ttgcggaaag tgttcggcat cgtgctgctc gccatcgcgg tgaaattatt ccgcacgcat
     2101 acgcacgtct ggcccggcaa ctgaaatgga acttgatctt tcctgcttcg aaaaatttcc
     2161 cgtgctggag acaaagcgcc tccgcctccg gaagataacg ccggaagatg cgccggctat
     2221 tttcgggttg cggaagaacg tccgcgtgca tgatttcatt taccgcccgt tgcagacgaa
     2281 gatcgaagaa gccgaagaca tcgtgaagcg cgtcgagaac ggctggctga cgcggcagag
     2341 cataggctgg gcggcggagc ataaggaaac ggggacgctg cttgccgcct gcggattcaa
     2401 ccgcatcgaa gcggaaaatc tccgtgcgga gatcggaggg gaaatgcatc ctgatttctg
     2461 ggggaagaat ttcgcgcagg aaggtctcgg cgcgatcatc cgtttcggat ttgaaacgat
     2521 gaaccttcac acggtggaag cccgcgtgat cagcggcaac cgcagcgcca tcgcattgct
     2581 cgaaaacaac ggcttcgtgc aggaaggaca cctgcacgag tacggatttt tccacgggaa
     2641 aagtttcgac ctgctgatct tcgccttgcg gaacccggga catcgcatcg tgtgatcaac
     2701 cgggataacc gcaggtaacg ccggcccggg ttttaccgtg aagtcataaa aacacaaaag
     2761 gcacagcgtc ttcgcgctga aagtgtattg ctgtgggatg atcccgcggg tacaataaat
     2821 ccgccttgcc tgcgtatctt tggcgactgg cttctcgcgt gaatatttat tccaggaaac
     2881 agctgtggaa atggctcctg ctggtagcag ccgcaattat tgttgcggcc tcgctgatct
     2941 acacctttca tctcgaacac aagatctccc aaagtgaaaa acgcaacgtc gagctgtggg
     3001 cggaagccgt gcgccgcaag gccatcatga tggaggagac gagccagctc tacagcaaga
     3061 tcagcaagga agagcggaaa aaaatggaga tcctggccaa cgccagccgc ctgctcgtga
     3121 atttcgatcc cgaccggctg gactcgaaag acacgtcgtt cttcgaattc attttccgcg
     3181 tgatcaagag caacgaaacc gttcccgcca tcgtcaccga cggaaaaggg cacatcgagc
     3241 aatggcgcaa cctcgacacg gcgaaaagca gggatcctgc atggcgctac gagcagctga
     3301 agatcatgcg cgcccagcac gattccattt tctacatcaa cgacacgctg acgaagaaaa
     3361 cctattacat gttttaccgg aattcgcaga tgttcgacga catacgcgaa tccttcgaga
     3421 gcaacgagcg ttccttcatc aaggaagtgg ccagcaatcc cgcgtctgcg ccggcgatct
     3481 acaccgactc cacttcctcc acgatcattg cgtacggcaa cctcgacacg ctgaagctgc
     3541 accgcgattc gctcgcgtac ctgcacgaag cgctgaatta cctgaaagag aacggcgaca
     3601 cgatcatggt ctccatcagc gaaggaagaa agaacgtgat ctactacatg gaatcgccgc
     3661 agctccagca gctgcgctat ttccccatcg tgcaggtgat cgtcgtcggc atgttcctgc
     3721 tcgtggcgta cctgctcttc agcacgtcac gcagggcgga gcagaacctc gtctgggtgg
     3781 gcatggcgaa agagaccgcg caccagctcg gcacgccgct ttcctcgctc atcgcgtgga
     3841 tggaatacct gaagatgaaa ggcgtcgatg agtcgatcct gaaagagatc ggccaggaca
     3901 tccgccgcct cgagatgatc accgaacgtt tttcgaagat cggctcacaa ccgcggctcg
     3961 ggaacgaaga cgtcgtggat gcgctctcgc acgctgtcga ctacatgcgt tcgcgcacat
     4021 cgaaaaccgt cacgttcaat tattctccgc ccgcttccat tttcgcgccg ctgaacgttc
     4081 cgctgttcgc gtgggtgatc gagaacctct gccggaacgc cgtcgatgcg atggacggga
     4141 ccggttcgat cacgatcgaa gtgttcgacg agcagcagtt cgtttacatc gacatcaccg
     4201 acacggggaa aggaattccg cgctcgaagc agaaaactat tttcgagccc ggcttcacca
     4261 cgaagcagcg cggctgggga ctcggacttt cgctctgcaa gcggatcatc gagcaatatc
     4321 acaaagggga gatctttgtg aaacgctcac tgcccgggaa gggaacgacg ttcaggatcg
     4381 tcctgaaaaa gtgagtttcg tcgggggagc acgccactta tcatcttttc accgtcattt
     4441 aacgaaaagc gggtttttta ttcatccgct gcaatatttt tgtgcccgct gcgacttcac
     4501 attagctaaa tcccgttgta tgaatttttt cctgagacga ttttttcttt tcctgctcct
     4561 tcttcctgct gcagccggag cgcaaaacaa gtatacggtc agcgggaatg tgaaaaacaa
     4621 aaagtccggt gaatccatta tcggtgcgat catccgcatt cccgaagtga agggcgcggg
     4681 cgccgccagc aatgaatacg gatttttctc catcaccgtt ccggaaggcg attatacgct
     4741 gaccgtgagc atgaccggct acaagccgca gagcatcccg ctttccctgc accagaatgt
     4801 tcaccaggat ttcatgctcg aggacacttc cttcacgcag aaagaagtcg tgatcaccgg
     4861 cgaccgcaaa gaccagaacg tcactgatcc gcagatgggc gcgcagaagc tcgacatgaa
     4921 agaagtgaac aagatcccgg ttctcttcgg tgaaaaagac gtgctgaaga ccatccagct
     4981 cctgccgggc atcaagtcgg gcggagacgg cggaagcgga tttttcgtgc gcggcggagc
     5041 agccgaccag aatctcatcc tgctggatga agcgccggtg tacaatgcgt cgcacctgct
     5101 cggatttttt tccacgttca attccgacgc gataaaagac gtgacggtgt acaagggaaa
     5161 tgcgcccgca caatacggcg gccggctggc ctccgtgctc gatgtgaaaa tgaacgacgg
     5221 caacaaccag cattaccacg tcagcggcgg actcggactg atctcttcga agctgaacgt
     5281 cgaaggtccg atcgtgaaga acaaaggctc ctttctcatc accgggcgcc gcacgtacgc
     5341 cgatcttttt ctcaagctgt cgaacaacga aacgacgaag aacagccagc tttattttta
     5401 cgacctcaac ctgaaagcga attacgagct gaacgaccgc aaccgcattt ttctttccgg
     5461 ttatttcggg cgcgacaaga tcggcctggc caatacgttc ggcatcgact ggggaaatgc
     5521 aactggaaca ttccgctgga accacgtgac gaacgaacgc ctgttctcga acacctcgct
     5581 catcttcagc aattacagct acaaaatctc gatcagcgga agcacagcca ccttcgacat
     5641 cacctcgaag atccgcgatt acaacctgaa gcatgaatac cagtttttcc cgaacccgaa
     5701 aaacagcatc cgtttcggct tcaacagcat ttaccacacg gtaacgccgg ggcagatcac
     5761 cgccgaagcg ggttcctcca tcaatcccgt gaagctgcag gaccgttacg gctgggaaaa
     5821 cgccgttttc atcagcaatg aatggaaagc gacggaacgc atttccatta cgtacggact
     5881 gcgcggaagc tcgttcagca ttctcggcaa gggcgatttt tattccttcg acaccgaagg
     5941 gaacgtttcc gacacagcgc attattcttc cgggcagatc gtgaagacgt atttcaatct
     6001 cgagccgcga atttcgggcg ccgttctcat caatgaaaaa agctcggtga agttttccta
     6061 ttcgcgcaac acacaatacc tgcacctgct ttccaattcg acatcgacga gcccgacgga
     6121 tctctggatc atgagcagcc cgatcatccg gccggagctg tgcgaccagt acgcgctcgg
     6181 ctattaccgg aatttccgcg acaacacgat cgaatgcagc gcggagatct attacaaaac
     6241 catgcagcac cagatcgatt acaaaaacgg cgcgaacacg cagggcaatg atctcgtgga
     6301 aggagaactg ctgttcggca tcggccgcgc gtacggtctc gagctcttcg cgaaaaagaa
     6361 ctacggaaaa ttcacgggct gggtcagcta cacactttcg aaaagcgagc ggcagatcgc
     6421 gggcatcaat gacggcaact ggtacccggt gaaacaggac cgcacgcatg acgtctcgat
     6481 cgtgctgatg tacgacctca cgccgaaatg gtcgatcgcc gcaacgtggg tctacaacac
     6541 cggcgacgcc gttacgttcc cgagcggaaa atattacgtc gacggtcagg tgcagttcta
     6601 ctacacggaa cgcaacggct accgcatgcc tgcgtatcac cgcatggaca tcggcgcaac
     6661 gtggcagcgg aagaaaaccg acaagttcga atcgagctgg aatttttccg tgtacaatgt
     6721 gtatgcgcgt gagaacgcat acgtgatcac cttccgtcag gatccgaacg atgcgtcaaa
     6781 aacacaggcg gtgcagacat cgcttttccg tctcgttccc tctgtcactt acaacttccg
     6841 tttctgaacc ggcatttttt cacgctatga aaaaaatatt cttccctttg ttcgccgttt
     6901 tcgtcctgct ggcttcatcc tgccagaaag tgatcgacgt cgacctcaat tccaaagatc
     6961 cgcagatcgt gatcgaaggc aacatcaccg atcagccggg tccgtatacc gtgagcatta
     7021 cgcaaacggt caacttcagc gaatcgaata cattcccgcc ggtgacgggc gcaacggtta
     7081 acattgccga caacgttgga aattctgaaa cacttacgga aaactcaccc ggcgtttacc
     7141 agacgaattc cttccagggc gttccgggcc gcacgtacac actgacggta atcaccaacg
     7201 ggaaaacgta tacggcgcag agcacgatgc cgtccgccat cccgctcgac agtcttctgc
     7261 tcgaaccgaa ccagtcgatc ggctcgccgt actatatcat cccgcttttc caggatcccg
     7321 cggggaaagg aaattattac cgctgcgtgg aaacgatcaa ccatgtgcgg aacaacggca
     7381 tctttctttt cgacgaccag ttcagcgacg gactgataaa cggcgaacct attcttgatt
     7441 ttacaacgga actggcgcac aacgattcgg tcgatgtcga actgcagtgc ctcgacaaag
     7501 cgacatacac ttatttcttc agtctcgagc agacgctgac cggccagacc accgcaccgg
     7561 ccaatccggt ttcgaatatt tccaaccatg cgctcggtta tttcagcgcg cacacggttt
     7621 cgcggaaatc aacggtgatc ccttaagccg ggatgactga ggcaatgaag caaccgggaa
     7681 aaatttcacc cggccacttc attctatgat cattttaatg ccacacggaa ataatgatgc
     7741 tcatcaccgt gatggcgacg atgtgataac cggcatcgat gacccatacc ttgaacggct
     7801 tcatcaggta aagatggctc atcatgaacg ggatgcaggc gaccgcgctg agcgcaagcc
     7861 cgaccttgat cccggccata cagttcacag caccgagggc catgacgaag aacccgatga
     7921 gcatggccat ggcgatattc atcaccaggg tgatcagcat catcaccggc atttttttcc
     7981 tcatctcctt cttcgctgct tcatccgtag gcatctggat attgtgccct gccatccacg
     8041 gtttcccgaa aagaacggaa aaccagagag caccgagtcc gtaatagaca agaaccgaca
     8101 cgccgatagc gagccagttg gcatgggaga aaaagtgcat taacatggtt cagcggtttt
     8161 ttggtttaag aaaagataca tcatttttcc ctgcggatga aatcgaatga tgcggggccg
     8221 ctgttccgtc cgcccggaac ctgttcctgc gtccggtttt tcagcccgtt atacaacatg
     8281 aaaacccgga aggaaccgca aggggaacag gagttgccta tttttgaacc agctatttcc
     8341 gaagcacatg aacaaataca tcgcatttct ccgcgccgtg aatgtcggcg gacgcgtcgt
     8401 aaaaatggac gagctgaaaa aaatattttc gctgcccggg tttaaaaaca tttccacgta
     8461 catacaaagc ggcaacgtgc tgttcaaaac aaaagaaacg gaggttacgg ttttaaaaaa
     8521 gaaaacggag aacgcgctga agaaagcctt gagctacgag gtgcttgttt tcctgaaaac
     8581 cattcccgac atcgaaaaga tcatcaaacg gaacccgttc aaaaaagaga tggagggaat
     8641 gcatctttat gtttccatgc tttcggaaga acccgccagg gaaaatattc cgcttcttga
     8701 aaagtaccgc gcgccggaag agcggatcac catcgacggc atcaacgcat atattctttg
     8761 tccaccgaaa acgtacggca catcgaaatt atccaacagc atgctcgaga aaaagctgaa
     8821 agtgagcgcc accacgcgca actgggcaac gatgaacaag atcattgaac tgtaatgaag
     8881 tgaacagccg aaatgcacct tccgggcaac aagcgaaaaa aggttttacc cgcagcgcac
     8941 gaaaaacaag aataagctca atgtttaaac gttccgggtt tgaacgtttc gggcctgaac
     9001 gacaaactga aaacaggtaa ccggcaacaa tttcccccta tcgcccggca agttcctcct
     9061 cgcgcacatc atccacgttc agcatcggca ggcggtacat ctcgctcact tcgatcgacg
     9121 tgaaatcgaa ttcttccacc acttttcctt tcagcaaata gcagcccggg ccgcggaacg
     9181 ggaacgcttt cgccgatggc ggaaaatgca ccgtgtcgat ccacatgcct tccgtgtcga
     9241 ggaacgttcc gaaatgcatg cgttctcctt tcgatgttcc cgtgttcttc acggtgacga
     9301 gatagcccgc gatctccact gttttcccga tgtgatgacg aagatctttc gccgtcagcg
     9361 gcgaaggcag tttatcgcgc agcagcttaa acggggaaca taacgaaaaa ccgaacagcc
     9421 cgatatcgtc gaacgcatcg tcgagccagg tggacgtaag gccgggaagc cggtattcct
     9481 tcgggcgcac gtcgaaaagc tccgtgtggt gcggattttt tttcaacggg ttgatgagcg
     9541 tatgcgcttc ccacatcagc gcacgcttat gctctcccgt gaagcggaac gtatttgcgc
     9601 gcacaaggat gcgcagctgc tccacggaaa cgggaacgcg cttcacgaaa tcgtacaggc
     9661 tgcggaattc cccgctgcgt tcgcgttcgt tcacgatcgc gttgatcaca ttctgctcca
     9721 gctcgctgat catgccgaga ccgagataaa ttacggaacc tttgatcgcg cacagggcgc
     9781 cgctgttgtt cacgcacggc tgaacgatct ccgcgccatg catgcgcgct tcatgcacgt
     9841 aaaattccgt acggtaaaat ccgccgccgt tgttaagcgt ggcgaccatg tattccagcg
     9901 gaaaatacgc cttgaggtac agcgcctgga agctttccac cgcgtagctc gcggaatgtc
     9961 ctttcgaaaa cgcgtagttc gcgaagcttt caatctggtt ccaggtatcg gtgatggttt
    10021 tttccgcgta acctttcgcc aggcaattcc tgaaaaaatt ttccttcaca cgccagaatt
    10081 cgttccgctg cttgaacttc cacgacatgc cgcggcgtaa ataatccgct tccgccagcg
    10141 aaaggccggc gaaaaaatgc gccaccttga tcacgtcttc ctggtacacc atcacgccgt
    10201 acgtttcttc gagcagatcg tacagctccg ggatttccgc gcgggctttt tcccgtttcg
    10261 ccttgtcgcg gtagcgcagg atgtattcgc gcatcatccc gcttttcgaa acgccgggac
    10321 ggatgatgga gctcgccgca acgagacgga gataatcatc cgcctgcagc tttgcgagca
    10381 gcatgcgcat ggccggcgat tcgatgtaaa aacagcctat gcatttcccg acactcagca
    10441 gctgtttcac tttttcatcc tgcatgaact tccgcgtgtc gtgaatatcg atctgcgcac
    10501 cccggttctc ttcgatgatc gtgagcgcat cttttatctt cccgagtccg cgctggctga
    10561 ggatgtcgaa cttgtacagc ccgatgtctt ccgcttcgag catgctgaac tgcgtggtag
    10621 gataaccttt cggcggcatg aatgtcgcgg tgtagcaggt catcggtttt tccgagatca
    10681 ggatcccgct cgaatgaatg ctcaggtgac tcgggaaccc tttgatgaat gagctgtagc
    10741 gcagcacaag acgcccgagc tcgtccgcat caccatgcgt gtgcgtagcc tgcaggcgat
    10801 cgatctcgcc cgcgggaaga ccgaacactt ttcccagctc gcggatcacg gcgtcgtgct
    10861 ggaacgtggc gtacgtagcg agcagggcgg tgcgttcatg gccaaaacgt tcgaaaatgt
    10921 agcgcgtcat gtcgtcgcgg tcggtccagg aaaaatcgat gtcgaaatcc ggcgcgttgc
    10981 tgcggaactg gttgatgaaa cgctcgaagt aaagatcgag ctcgatggga tcgacatcgg
    11041 tgatgcgcag gaggtacgcc accatgctgt ttgcgccgct tcctctcccg acgtaataat
    11101 aatttttccg ctgcgcatag cgcacgatgt cccagttgat gagaaaatag gaagcgaaat
    11161 tcatctgcgt gatcacctgc agctcttttt ccacgcgctg cagaacttcc ggcgaaggtt
    11221 ctccgtaacg gtactgcagc ccgtgcatgc attccatgcg cagcagctcc gcatcttccg
    11281 tcacgctgtc ggtgtaatgc ttcaggttct tattcgcgag cttgccgtat tcgaattcga
    11341 tgcggcaatc atccagcagc cgttctgtgt tctccacgac ggaaggatac agcgcatacg
    11401 aggcaagcag ctcgtttttg tgcggaatga tctcgtcagc cgtcgcctgc tcttccgcgg
    11461 gcagtttgct cagcagcgtg ttctgatcga tggcgcgcag caggcgatgc gcgctgtggt
    11521 ggcgtttgtg aatgaacgtg agcggctgaa gcacgacgag cttttccggg tgccggcgcg
    11581 ccggcaaaaa cgcgatgcgc tgcaggtctt tcagcgaaac accgatgaat tcattttccc
    11641 gcagcggcca tccttcataa acgttcgcgg gataaacgac atacgcgtgc cggaattccg
    11701 gtgcggcagg accgaatttt tcatctgcgt gcaaatgttt ggagagatgc ccgttcagct
    11761 cgcggaaacc ttcgttgtcg cgcgcgatac cgacatagca ttgctttacg ccattcctga
    11821 aatcgatgcc ggcgaccggc ttgagtccgc gcgcagcgga aagtcgcacc gtgtccagca
    11881 ccgccgacgt gttgttgata tcggacacga cgaactgcct gtatcccttc gaggaaattt
    11941 catccagcat ttcttccacg gacaatgttc cgtagcagaa gctgtagaag gtatggcagt
    12001 tgagaagcat tgctgtgtgt tttcatcacg ggagcacagg ttacgtctgc cgcccgttga
    12061 acgggttgaa atccctgtgc gtgatgccca ttcccgacgc gcgctgcacg gcatttttcc
    12121 cgaatcgctg ccgcatcctg tccatcgcct ggtagagctg cagctgctcg atcgtgtctt
    12181 cgaaaagatc gaactggtaa ttgccctgca cgaggtgact gaaacgcacg ccgatgaggc
    12241 ggatgagcat gcgtttctgg tataattttc cgaaaagctc tttcactttt tcgatgagca
    12301 tgtgatcgag cgaagtgtac gggatgcgca tctgcatcgt gtacgtgttg aaatcggaat
    12361 agcggatctt caccgtcaca cacgacgtga gtttctgctc gctgcgcagc tggaacgcga
    12421 gcttctccgt catgctgatg agcacggaac gcaggtacga aacgtcgatc gtgtctttct
    12481 cgaacgtgca ttcggtggaa atggatttcc gttccgaata cggttccacc ggcgtattgt
    12541 cgatgccgtt cgcttttttc cagagcgcgg tcccgttctg cccgagcacc tgcgtcatca
    12601 gctccatggg catttcctgc acggttttta ttttttccac gcccattccg cgcaggagct
    12661 ggtaggtttt atcgccgacc atggggattt tccggatgga cagcggcgca agaaaatgtt
    12721 tttccgttcc gaacggaatt tccttctgcc cgttcggttt tgcttccccg gttcccactt
    12781 tggaaaccgt tttattcgcc gacatggcga acgagatcgg caggcccgtt tcccgcgtga
    12841 tcgttttgcg cagatcggtc gccagcttgt agcttccgaa aaaacgttcc gttcccgtca
    12901 ggtcgatgta gaattcgtcg acggatgttt tttcatacac cgggacgctt tcgcggatga
    12961 tatcggtgac gacgttcgaa taatagctgt actgctcatg gtcgccgtgc ttgatgatcg
    13021 cgtgcgggca gagcatacgc gccgtacgca tcggcatggc ggaatgaatg ccgaactcac
    13081 gggcttcata gctgcacgac gccaccacgc cgcggtcgct cgtaccaccg atcagtacgg
    13141 gctttccgat gagcgagctg tcctgcaaac gttcgacgga gacgaagaac gtgtcgaggt
    13201 ccatgtgaac gacggtgcgg ttcatgcggg tcaggataat gcggggaaac gcccttgcaa
    13261 gttgcagatt tatttagtaa attggcgcta aaattattag caatgtcgcg actggccacc
    13321 aacctgcgga tactgaggaa aataaagagc tcgtcacagg aaaccgttgc cgaagatctc
    13381 ggcattccac gttcccgcct gggctcgtac gaggagggcc gcgccgaacc gccgtgcgac
    13441 atgctcctcc gcatttcgga ttatttccac gtttcggtcg atgcgctgct gcgcggtgat
    13501 ttttcaaaaa cagatcccga aagcctgatg aagatcggga agaaccgttt tctttttccc
    13561 gtgctggtcg acagcaaagg caacgattac atcgaggtcg ttcccgtgaa agcttccgcc
    13621 gggtatctca acggttattc cgacccggaa tacgtggaaa agcttcccgt gatgagcctt
    13681 cctttccacg tcaccggcaa gcaccgctcg tttcccgtga aaggcgattc catgcccccg
    13741 ctgaaaaccg gcgatttcgt gatcgggaaa ttcgtggagt cgctgaacgg gatcgtgaa
//
LOCUS       DASTSF010000287         6010 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1371622,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000287 DASTSF010000000
VERSION     DASTSF010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 6010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6010
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1371622"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..360
                     /locus_tag="VFU15_08370"
     CDS             <1..360
                     /locus_tag="VFU15_08370"
                     /inference="COORDINATES: protein motif:HMM:NF014232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HEU4717835.1"
                     /translation="SMKKTMDETDRRRKKQIQYNFEHNITPQQIVKSKDAMSAVLKMT
                     RGSGRKENYYVESENVNDLLAAEPVLHYMTKEQLEKAIRNTRKAMEAAAKEMDFMEAA
                     RLRDEMQAMERMMNEKK"
     gene            complement(443..619)
                     /locus_tag="VFU15_08375"
     CDS             complement(443..619)
                     /locus_tag="VFU15_08375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717836.1"
                     /translation="MRKTLLKFFTTKALMIVLAFAAVGFVAESCGPKHACGTKHQKRQ
                     RNKRIKHNTNFMTY"
     gene            complement(697..2787)
                     /locus_tag="VFU15_08380"
     CDS             complement(697..2787)
                     /locus_tag="VFU15_08380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717837.1"
                     /translation="MKLSIVTEAPAWYVLFCVAAGLLYSGILYYRENRNTAVPRWLKN
                     VMAVTRFTVIFILTFLLLGPMLKTIFREVQKPVIVLAQDASGSILMNKDSAFYRSQYP
                     GKINALVEALSDKYDVQTYSFGDHFREKTDFSFRDKQTDFTSLFDEIESRYSDRNLGA
                     VIVASDGIYNEGQSPVYAAEKIHAPVFAIALGDTTVRKDLILTKVVHNRIAFLGNTFP
                     IEVVADAHRCMGEKSILTVTKGGQTLASKPVDINADPFDITIPVELTASSPGLQRYTV
                     TLSVINGETNVQNNTQDFFIDVLDSREKILIVCANPHPDVGALKEAIETNDNYQVETY
                     TLAQFDKPVDKYNLAILHGIPSDQAPGQKLIADLAAANIPVWYITGASGRFSGFNSLG
                     AGVNVQVSGSSANDCEAYPADGFPLFNLSSQATDYIPKFPALTCPFANYQVSPAVNPL
                     MKQKIGVLKTDYPLWVFSQEGTRKVGIIFGEGIWKWRLHDYADHGNHDIFNELVGKTV
                     QYLSVRDDKSFFRVSSKTNYLENESVQFDAEVYNQSYELINTPVVTIDIISEDGKRSP
                     RTFTATANAYHLDAGQFPVGEYSYEATTKVGDKVYTQKGKFSVSPLFIEIANTTANHQ
                     VLFNLCHRHNGEMVAPSSIDKLVQILNSREDIKPVIYNPKKLVDLINLWWFFLVLMGF
                     LTLEWFMRKRNGLY"
     gene            complement(2834..3580)
                     /gene="fabG"
                     /locus_tag="VFU15_08385"
     CDS             complement(2834..3580)
                     /gene="fabG"
                     /locus_tag="VFU15_08385"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015782609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEU4717838.1"
                     /translation="MKLLEGKTAIITGASRGIGHGIALAFAKHGAHVAFTYLSSVEKG
                     KALENELGAHGIKAKGYQSDAGDFKAAEELVNAVVSEFGTVDVLVNNAGITRDTLLMR
                     MSEQQWDEVIHANLKSVFNLTKAVQRPMLKQRKGSIINMSSVVGVRGNAGQANYAASK
                     AGIIGFSKSVALELGSRNIRCNAVAPGFIETEMTAALDPKVVEQWREGIPLKRGGTPE
                     DVANVCLFLASDMSAYVTGQTLNVCGGMLT"
     gene            complement(3800..5389)
                     /locus_tag="VFU15_08390"
     CDS             complement(3800..5389)
                     /locus_tag="VFU15_08390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717839.1"
                     /translation="MEKLHQLLHSLQPNQVRVLKNYLTSFSSRNDPDSKSWELAEYVL
                     SKTKKAPTLVQASHYLYGKKAINSINRLKNRLFDKVLNSLLIDISTDAMTREDDTHPV
                     QIRIRKKMLLFDLLKYTATRGTVAVDMIQDVIKDAREYEFFPILIDALTIYKPMISHL
                     KSKREADKIDSEITKYEKAKYYNIRAKDIVTELGQYSTFRTKLEKEKILNFLKDSIEE
                     LRGYYSEVGGRLLLDHLRTCEMALLQVAGQLSEGISLAKEHVRFLESNKIIDRKNRIG
                     VWNDYIAHLEIDNENYDEAMKYLEKAASCFTSSPTNLAVNRSIQMEVYFHKGNYDKAL
                     EISADLIENQAKVIGTFRRDTFIFFQASCYFMKKEYTRCNDLLNERFELTRDKLGWDL
                     NIRFLRIMTMIEMGKPDLAMLQVDALRKNFERFQHRKDITERDKMIIRLFFQMFLEGF
                     AFQYPRNKTNELLLKLKEKGKLYSWEALKPEKIPIHVWALSRYQYKGVLPREEEEDDE
                     EDGEGKFGGKRRPAQKAKSNA"
     gene            5512..5805
                     /locus_tag="VFU15_08395"
     CDS             5512..5805
                     /locus_tag="VFU15_08395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717840.1"
                     /translation="MKTRTFLLAAALTLASTFAVSGNLKSNTSNEKINSAASYCGVTA
                     QDICKYMSSFDITITNIIPIQGTCNVLTPDTMGKTWIVYIDNGIIIGHETWEG"
BASE COUNT         1418 a         1497 c         1530 g         1565 t
ORIGIN      
        1 tccatgaaga agacgatgga tgaaacggac cgccgccgta aaaaacagat acaatacaat
       61 ttcgagcaca acatcacccc gcagcagatc gtgaaatcga aagacgcgat gagcgccgtg
      121 ctgaagatga cgcgcggttc cggcaggaaa gaaaattatt acgtcgaatc cgaaaacgtg
      181 aacgacctgc tggctgcgga acctgtcctg cactacatga cgaaggagca gctcgaaaaa
      241 gcgatccgga atacacgtaa ggcgatggaa gccgctgcga aagaaatgga tttcatggaa
      301 gccgcgcgcc tccgggatga aatgcaggcg atggaacgga tgatgaatga gaagaagtga
      361 gaggtcctgt gctgaacagc ttcatatttg ctcaaaaaaa aagcacagcg atcgccgtgc
      421 tttacgtttg ctgagatcag gctcagtacg tcatgaagtt cgtattgtgc ttgatgcgct
      481 tgttacgctg tcttttctgg tgcttggtgc cgcacgcatg cttgggtccg caggattctg
      541 caacaaaacc gacggccgca aaagcgagaa cgatcataag cgctttggtg gtgaaaaatt
      601 tcaggagcgt cttcctcatg gtgtgctgtt ttatgtgttt ggtaaataca attatacgaa
      661 aagaagaccc gaaaagcaat ggctgtgaat gaattgtcag taaagtccgt tgcgtttgcg
      721 catgaaccat tccagcgtaa gaaatcccat cagcacgagg aagaaccacc agaggttgat
      781 caggtcgacg agctttttag ggttgtaaat aacgggcttg atgtcttccc tgctgttcag
      841 gatctgcacg agcttgtcga tggatgaagg cgcgaccatt tccccgttgt gccggtgaca
      901 gagattgaaa agcacctggt gattcgcggt cgtgttcgcg atctcgatga acaacgggct
      961 cacagaaaat tttcctttct gcgtgtacac cttgtccccc actttcgttg tcgcttcgta
     1021 gctgtattcc ccgacaggga actggcccgc gtcgagatga taggcattgg cggttgcggt
     1081 gaatgtacgc ggcgagcgct tgccgtcttc gctgatgatg tcgatcgtaa cgaccggtgt
     1141 gttgatcagc tcatagcttt ggttgtaaac ttccgcatcg aactgaacgg attcgttttc
     1201 gaggtaattt gttttggaag aaacgcggaa aaagcttttg tcatcgcgaa cggaaagata
     1261 ctgcaccgtt ttcccgacga gctcgttgaa aatatcgtgg ttgccgtgat cggcgtaatc
     1321 atgcagccgc catttccata ttccttcccc gaaaatgatg ccgacttttc gcgttccttc
     1381 ctggctgaac acccaaagcg ggtaatccgt tttcagcaca ccgatcttct gcttcatcag
     1441 cgggttgaca gccggtgaaa cctggtaatt cgcaaagggg caggtgagcg cgggaaattt
     1501 cgggatgtaa tcggttgcct ggctgctcag gttgaacagc ggaaatccgt ccgccgggta
     1561 agcttcgcaa tcgttggccg atgatccgct tacctgtacg ttgacgcccg cgccgagcga
     1621 gttgaacccg ctgaaacggc ccgaagcccc ggtgatgtac cacacgggaa tgttggcagc
     1681 cgcaagatca gcgatcagct tttgccccgg cgcctggtcg gaaggaatgc cgtgcaggat
     1741 ggcgaggttg tatttgtcga ccggcttgtc gaactgcgcc agcgtatatg tttccacctg
     1801 gtaattgtcg ttcgtctcga tcgcttcctt cagtgcgcct acgtccggat gcgggtttgc
     1861 gcagacgatc aggatctttt cgcggctgtc gagcacatcg atgaaaaaat cctgcgtgtt
     1921 gttctgcaca ttcgtttcgc cgttaatgac agagagcgta acggtatagc gctgcaggcc
     1981 cggtgaggaa gctgtcagtt cgacggggat cgtaatgtcg aacggatcgg cgtttatgtc
     2041 aactggtttt gatgccagcg tctgtccgcc tttcgtgacg gtaagaatgc ttttttcacc
     2101 catgcagcga tgcgcgtccg caacaacttc aatgggaaat gtgttgccga ggaaagcgat
     2161 gcggttatgg accactttcg tcaggataag atctttccgt actgtcgtat cgccaagggc
     2221 gatggcgaaa accggcgcat gtattttttc cgcggcgtaa acaggacttt gtccctcgtt
     2281 gtatatgccg tcgctggcaa cgatcactgc gccgaggttg cgatcagagt aacgcgactc
     2341 aatttcatcg aaaagcgaag tgaaatccgt ttgcttgtcg cggaagctga agtcggtctt
     2401 ttcacggaaa tgatcgccga atgaatacgt ctgcacgtcg tacttgtcgg aaagcgcttc
     2461 gacaagcgca tttattttgc ccgggtactg gctcctgtaa aatgcggaat ccttgttcat
     2521 caggatcgaa cccgacgcat cctgcgcaag gacgatgacc ggtttctgca cctcgcggaa
     2581 aatggttttc agcatcggcc cgagcagcag gaaggtgaga ataaaaatga ccgtaaagcg
     2641 tgtcacggcc atgacgtttt tcagccatcg cggaacagcc gtgttcctgt tttccctgta
     2701 atacagaatg ccggaataca gaagtcccgc ggctacgcag aaaagcacgt accacgccgg
     2761 ggcctccgta acgatggata gtttcatttt attccggggt tggttccgta ttcatggcgg
     2821 aacaacggaa agatcacgtg agcattccgc cgcacacgtt cagcgtctgg cccgtcacat
     2881 acgccgacat atccgacgcc aggaacaggc aaacgttggc aacatcttcg ggtgtgccgc
     2941 cccgtttcag gggaattcct tccctccact gctccacgac tttcgggtcg agcgccgcag
     3001 tcatttcagt ttcgataaac ccgggggcaa ccgcgttgca gcgtatattt cgcgaaccca
     3061 gttcgagcgc cacggacttg ctgaacccga tgatgcctgc tttggatgcc gcgtaattgg
     3121 cctgtcctgc gtttccgcgc acgcccacca cggagctcat gttgatgatg gagcctttcc
     3181 gctgcttgag catggggcgc tgaacggctt tggtcaggtt gaacacggac ttgaggttgg
     3241 catgaatgac ttcgtcccat tgctgttcgc tcatgcgcat gagcaatgtg tcgcgcgtga
     3301 tgcctgcatt attgacaagg acgtccaccg tgccgaattc ggagaccacg gcgttgacca
     3361 gttcttccgc cgctttgaaa tcgccggcat ccgactggta acctttggct tttattccgt
     3421 gtgcgccaag ttcgttctcc agcgcttttc ctttttcgac agaagaaaga taggtgaacg
     3481 ccacatgggc tccgtgcttc gcaaaagcaa gtgcaattcc gtgaccgatc ccgcgggaag
     3541 cgcctgtaat gatcgccgtt tttccttcaa gtaatttcat ccgttttgtg atttatgaat
     3601 gcagccaaag ataacagaat tgatccggag aaaccggcaa atcaggcagg cggaagaaag
     3661 agcattacca tccaaagacc ttaatataca ttctatcgaa aaaaagtgaa cgggcggttg
     3721 cattgcatta agtaagcatt atatttgtgc agttctgccg cccggcagaa caatactaag
     3781 aagagcagcg cttttggggt taggcgttgc tcttcgcttt ttgtgccggc ctgcgtttcc
     3841 cgccgaactt tccttcaccg tcttcttcgt catcttcctc ttcctccctc ggaaggacgc
     3901 ccttgtattg atagcgggaa agcgcccata cgtggatcgg gatcttttcc ggcttgagag
     3961 cttcccagga atacagcttt cccttttcct tgagtttgag cagcagttcg ttggttttat
     4021 tccgcgggta ctggaacgcg aacccttcga ggaacatctg gaagaacagg cggatgatca
     4081 tcttgtcgcg ctccgtaatg tccttgcggt gctggaagcg ttcgaagttt ttccgcaaag
     4141 catccacctg cagcatggcc aggtccggct tgcccatttc gatcatcgtc atgatgcgca
     4201 ggaagcggat gttgaggtcc catccgagct tgtcgcgcgt cagctcgaac cgttcgttga
     4261 gcaggtcgtt gcagcgggtg tattcctttt tcatgaaata gcagctcgcc tggaaaaaga
     4321 tgaacgtatc tcttcggaac gtgccgatca ctttcgcctg gttctcgatg aggtcggcgg
     4381 aaatttcgag ggccttgtcg taattgccct tgtggaaata cacttccatc tggatggaac
     4441 gattcacggc aaggtttgtc ggactgctgg taaagcagct ggccgctttt tcgaggtact
     4501 tcatcgcctc gtcgtaattc tcgttgtcga tctcgaggtg agcgatgtag tcgttccaga
     4561 caccaattct gttctttcgg tcaatgatct tattggattc gagaaacctg acatgttctt
     4621 ttgccaggga aattccctcg cttaattgac ctgctacctg aagcaaagcc atttcacatg
     4681 ttctgagatg atccaacagc agacgtccgc caacttccga ataataacct ctcaactctt
     4741 caatggagtc tttcaaaaaa ttcagaattt tctccttttc gagttttgtc ctaaatgttg
     4801 aatactgtcc cagctcagtc acaatatctt tcgctcttat attgtaatat tttgcttttt
     4861 cgtatttcgt tatctcggaa tctattttat ctgcttcacg cttggatttc aaatgagaaa
     4921 tcattggttt gtatatagtc agcgcatcga tcagaatagg aaagaattca tattcccttg
     4981 cgtccttaat aacatcctgg atcatatcga cagcgacagt tcctcgtgtg gccgtatact
     5041 tcagcagatc gaacaacagc attttctttc ggatccggat ctgcacagga tgggtatcgt
     5101 cttcccttgt catggcatcg gtgctgatat cgatgagcag ggaattcagc actttgtcga
     5161 acaggcggtt cttcaggcgg tttatgctgt tgatcgcctt tttgccgtac aggtaatggc
     5221 tggcctgaac gagtgtcggg gcctttttgg ttttcgacaa tacgtattcc gccagctccc
     5281 agcttttgct gtccgggtcg ttccgggagg aaaatgaagt gagataattc ttcagcaccc
     5341 ttacctggtt cggctgcaag gaatggagca actggtgaag cttttccata ttatgcttac
     5401 aaaataacac aaattcggct gtcttatccg ggcttatttt tccataaaaa gaaacagtcc
     5461 gtggcccacc tttgttccat cattcactga caaccataaa aactacagga catgaaaaca
     5521 aggaccttct tactcgctgc ggcgcttacc ctcgcgtcga cgtttgccgt ttcaggcaac
     5581 ctgaaaagca acacctccaa tgaaaaaatc aacagcgccg cttcctattg cggggtaacc
     5641 gcgcaggaca tctgcaagta catgtcgtca ttcgacatca ccatcaccaa cattattcca
     5701 atccagggaa cctgcaacgt gctgaccccc gacacgatgg gcaagacctg gatcgtctac
     5761 atcgacaatg gaataataat aggacatgaa acatgggaag gataatcatc cgtcaataaa
     5821 atgataagtg cgcttacttt cctttaaaca ttagggacag agaagcgaca ctttcgaaaa
     5881 aacagatcaa ataaatctta ttcgaatgtc ttttggccgc cggaaacttc cggcggcttt
     5941 agtgttatta cagccaattt tgtatggtaa ggttaatctt ttaccttcat ttccccaaat
     6001 aaaaacctat
//
LOCUS       DASTSF010000288        10356 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1490755,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000288 DASTSF010000000
VERSION     DASTSF010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 10356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10356
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1490755"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..829)
                     /locus_tag="VFU15_08400"
     CDS             complement(<1..829)
                     /locus_tag="VFU15_08400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717841.1"
                     /translation="MKLLILSPDAYSVFHTDTSYIFGGIEIETGYHARGLAKMGVDVF
                     VVTRDQGREAHLCEGVHLLPHPRLKGEGYWEKRRSFGGRIQYRLFGDRGKFSTADELY
                     DHIAPDACYVMGMAPETLHLARYCRRKNCVFIFRIAHDLDLGDETFDAEKMKAWAGVS
                     PAEVREVIETAAEVLVQTPFQREMLEKRFGRSGEMMFPPIPTDVPQDGVAKEYDVLWV
                     GKSNTFKRPEKLAGMARRMPGAKFCMVFNKVNALQWEEVRSQLGPNVTLLESVPADRI
                     "
     gene            complement(881..1810)
                     /locus_tag="VFU15_08405"
     CDS             complement(881..1810)
                     /locus_tag="VFU15_08405"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU4717842.1"
                     /translation="MNPLVSVIVPNYNHAAFLDRRMESIFAQTYTDFEIILLDDHSSD
                     ISVSILERFRGHAKVTHIVYNEKNSGSTFLQWEKGISLASGKYIWIAESDDFCETDFL
                     AIAVNRLEGGADLFMGLPLVVDADGTLAGETNPWLDERFPERWKQELSVDAAREVREM
                     LFVKNTIPNASAVVFRKTEKLPGYFRTISGMRFCGDWLFWMLYLLDAKTFSFSPSART
                     FFRRHPSVTRLQNAYGERNLEVARILKFILAHPLSAGERKFIERYFFRHHFHVYERKN
                     IRANFRLALVQVRISARFLFSWIAYYSAFSDRG"
     gene            complement(1914..2981)
                     /locus_tag="VFU15_08410"
     CDS             complement(1914..2981)
                     /locus_tag="VFU15_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004307431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 99-like
                     domain-containing protein"
                     /protein_id="HEU4717843.1"
                     /translation="MSKLRPIAIYLPQFHPFAENDEWWGKGFTEWTNVTKTVPRFKGH
                     YQPHLPADMGFYDLRVHQALISQAELAKQYGIYGFCFYHYWFNGKRLLERPLEDMLAK
                     GTPDFPFCLCWANENWTRRWDGLEQEVLMKQDYSEEDDRRHINFLARYFRDPRYIRVD
                     GKPVFIVYRPKLFPDIRRTAETWREECRKNGVGEIYLMYMQSFSDRRDPAELGFDAAV
                     EFQPDFSFLPQPVRPQRSLWSRLAGAKATGDNIFLYKDVVREMLRKPAVAFKRYPGIF
                     PSWDNSPRRSTGATIIHDSTPGEYGKWLRHIIAGFKPYSVDENFIFINAWNEWAEGNH
                     LEPCRKWGRAYLEETQKALTA"
     gene            complement(2974..4044)
                     /locus_tag="VFU15_08415"
     CDS             complement(2974..4044)
                     /locus_tag="VFU15_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007842527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 99-like
                     domain-containing protein"
                     /protein_id="HEU4717844.1"
                     /translation="MIRPIAIYLPQFHPTPENDKWWGKGFTEWTNVTKAKPLFRDHYQ
                     PRLPSDLGFYDLRAIETIEEQAALAREYAIHGFSFYHYWFSGKRLLHDPLDLLLETKR
                     PDFPFLLFWANESWSRRWLGEERDVLIRQEYSREDDLHHAEWLCGRVFPDKRYIRVDG
                     KPVFVIYRPNDFPDIGETLKIFRETAVKKGFPGLYLIGNNSHTLKKPEVFDTVLNFEP
                     QLGLLQQAFHDKWSWRRLLHNVSRFGLYSGTLKLYSYAETKNLMKKRRLPEKFVPCPF
                     VGWDNTARRGEKGIILVGQDTQPFEDSLRWAKEIAMKLPGEEQFVFINAWNEWAEGNH
                     LEPCRRYGRAFLETVKKVFAGE"
     gene            complement(4046..4807)
                     /locus_tag="VFU15_08420"
     CDS             complement(4046..4807)
                     /locus_tag="VFU15_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU4717845.1"
                     /translation="MLDLPRNIRAAVKESRLERNSGYCCICRQETEFIIYNEWLRDNY
                     RCKRCQSIPRNRALVNVLDLYCKDWPNLDIHESSPGGCVSELLKKNCRNYTSSHYYDD
                     VPRGEYRNGHRSEDLSRMTFPDASFDLLITSDVFEHVFEPDKAFREVARVLRPGGMHI
                     FTMPWVPAHKKSVQRARLRSDGSIEYFREEIYHGNPIGKGKGALVTFDWGLDFTDFIF
                     EHGGMTTTIYLETDRKKGLDGQFLEVFISRKPQEK"
     gene            complement(4894..5703)
                     /locus_tag="VFU15_08425"
     CDS             complement(4894..5703)
                     /locus_tag="VFU15_08425"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU4717846.1"
                     /translation="MSEPLISVIVPAYNSGPAIRPCLDSVFGQTLQNVEVIVVDGQSS
                     DETVDILRAYEKEKKLRWKSEKDDGIYDAINKGIRMAKGRWMYVLGSDDRLYENTTLE
                     KVAPFLEKAGSGVVYGNVKIKGDAGWAKDGEVHDGEFTTEKLLRANICQQSVFYHRDV
                     FEKFGYFNPEYKVCADWDFILRCAAGTSLVYMDVIVAEFSGGGTSAVRKEEKFYEELP
                     ANLYRYFGLRVFRKEFRPAGWRFRKLSSEYAAKGKKWKSFFFGRVARRLGS"
     gene            complement(5706..6608)
                     /locus_tag="VFU15_08430"
     CDS             complement(5706..6608)
                     /locus_tag="VFU15_08430"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU4717847.1"
                     /translation="MQYPKISIVTVSYNQAGFLESTIRSLLDQAYPALEYIIIDGGST
                     DGSVDIIRKYEKHLAFWISEKDNGMYDGLQKGLSRATGEVMAWINSDDLFHPGSLWVA
                     GEIFSAMPQVEWIQGMASVIDETGRTVMVKNFRKWSKYNFFLGDPEHIQQESTFWRRS
                     LWEKAGARLDTSLRYAGDYELWLRFFDHAQLHSVGTTLGAFRFRTKNQFTLEKMDDYN
                     REANAALAARLGRLSEEEKKTLKKIRRYHERQSPPAFLRKKRLQEYKSLFGFPLPISY
                     DRKNAAFRLGDFAITPPPGNETCL"
     gene            complement(6676..7392)
                     /locus_tag="VFU15_08435"
     CDS             complement(6676..7392)
                     /locus_tag="VFU15_08435"
                     /inference="COORDINATES: protein motif:HMM:NF021850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative capsular polysaccharide synthesis
                     family protein"
                     /protein_id="HEU4717848.1"
                     /translation="MGKVGTTTVYSHLKTQHPDLPVYHLHFMSDFWVNQKLPSLDPAF
                     HVNIEVARQVQQALKEQPGKRIQIITMVREPMSREISDIFQNWKGLFGVSSVNDLTFS
                     RLKEYLDAHDHEYVLNWFDTEFKAWTGFDIYSVPFNKEKGYSIYHTGKADILCMKTEA
                     MSTCFRKAMREFTHLDIVPVRSSNTSESKEGKQLYSELMSQYEPSPGKLDALYNSKYV
                     RHFYTESEIDAFRRKWSRAK"
     gene            complement(7555..8313)
                     /locus_tag="VFU15_08440"
     CDS             complement(7555..8313)
                     /locus_tag="VFU15_08440"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HEU4717849.1"
                     /translation="MKNVLKALFRMLGLEVRRSGTPAVQPQVARTKRDEDSMEAGMSR
                     AKKMHGVEPSAIIDLGAAAGSWTEKACTFWPDAYFLLMEPLEERKEELSALAAAHPKF
                     RTVFAAAGDRVGKVKFVVAGDLDGSGVYDAESTGGDREVELTTIDHEVERLGLAGPFV
                     IKFDTHGFEIPILEGARETLKKTDLIIMECYGFRIAANCLLFPEMCIHMEKLGFRLSD
                     VIHPVRRPGDGMFWQCDAFFLRKDHPAFAKNTFA"
     gene            complement(8310..9497)
                     /locus_tag="VFU15_08445"
     CDS             complement(8310..9497)
                     /locus_tag="VFU15_08445"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEU4717850.1"
                     /translation="MGRTGITVFYHRIEAETWLGGANYFRNLEEVTARFLTDVELRGI
                     SYTDVKVFYPAFRDKFRVAMNRLLRRKEEPSFVSRGRRQYLESLSPGREICAFVLFSG
                     HFPMLADLPVIFWVPDFQVFHLPGYFKPEDIERRKKNYAAGAAKADIILLSSDAARKD
                     LNRFYPGHESKVKVLRFVVDIPGAVYEKDPRFLLEKYHLPEKFIYVPNQFWVHKNHAL
                     IIEALAVLKRRNHELHVVLSGSTLDPRNPGHYDSLLGLVKANGLEKNISVLGIIEKED
                     VAQLIRQSCFVINASRFEGWSTTVEEVKSIGKKILLSSLDVHREQDPPGASYFDPDNA
                     GELAELMLSAWNSSAAGPDAEMEKHARETLPLRLKGFAETFGDIVHSLMDADSRRKKE
                     QEK"
     gene            complement(9550..>10356)
                     /locus_tag="VFU15_08450"
     CDS             complement(9550..>10356)
                     /locus_tag="VFU15_08450"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEU4717851.1"
                     /translation="YGCDVTGADDERFFRYSGPGTYERIFSSVPFDCCVNASGSPGVG
                     YSLASPEKDYELNTQNVRLMLGAIRAHQPACRFINMSSAAVYGNPSRLPVSEDAMPAP
                     LSPYGKHKLEAEQVLETFHREHDLRTVSLRVFSVYGPRLHKQLFWDIFQKTKQSKKLE
                     LFGTGEESRDFIYIRDLVRALETVMENGAFAGECINVASGVETTIREAASVFLDSLGG
                     GFQLSFNGEVKAGDPKNWQADISRISGLGFKPAVTIAEGLALTVKDYRSV"
BASE COUNT         2346 a         2910 c         2615 g         2485 t
ORIGIN      
        1 cgatccggtc ggcaggaacg gattccagca gcgtaacgtt aggtcccagc tgcgaccgca
       61 cctcttccca ctgcagggca ttgaccttat tgaacaccat gcagaatttt gcgccgggca
      121 tgcgccgcgc catcccggcc agcttttcgg gccgcttgaa cgtgttgctt tttcccaccc
      181 acagcacatc gtattctttc gcaacgccat cctgcggaac atccgtcggg atcggcggga
      241 acatcatttc cccgcttcgc ccgaaacgtt tttcaagcat ttcccgctgg aatggtgtct
      301 gcacaagcac ttcggcggcc gtttcgatca cttcgcgaac ctctgcggga gaaacgccgg
      361 cccatgcttt cattttttcc gcatcgaatg tttcatctcc gagatccagg tcgtgcgcga
      421 tgcgaaaaat aaaaacgcag tttttccgcc ggcagtaccg tgccagatgc agcgtttccg
      481 gcgccattcc catgacatag caggcgtccg gggcgatgtg gtcgtacagt tcatctgctg
      541 tagaaaattt cccgcggtca ccgaaaagac ggtactgtat tcttcccccg aatgaacggc
      601 gcttttccca gtaaccctct cctttaagcc ggggatgagg caaaagatgc actccttcgc
      661 acaggtgcgc ttcccttccc tggtcgcgtg tcaccacaaa aacatccacg cccattttcg
      721 caagaccgcg cgcatggtat cccgtctcaa tttcaatgcc cccgaaaatg taggaggtat
      781 cggtatgaaa aacagaataa gcgtccggcg aaaggatcag cagcttcatc aggcagggta
      841 aaaatacgaa aacaatacag ccttggtttc cgcaagtcct tcagccgcga tcgctgaaag
      901 cggaataata ggcgatccac gaaaaaagaa aacgcgcact gatccggacc tgcaccaggg
      961 ccaggcggaa attcgcgcgg atgttctttc tttcataaac atgaaaatgg tgccggaaga
     1021 aataacgttc gataaatttc cgttcaccgg cgctgagcgg gtgagcgagt atgaatttca
     1081 gaatgcgcgc cacctccagg tttcgttcgc cgtaggcgtt ttgcaaccgt gtgactgacg
     1141 gatgcctgcg gaaaaatgtt cttgccgaag gagaaaaaga aaatgtcttc gcatccagca
     1201 ggtacagcat ccagaacaac cagtcgccgc agaaacgcat cccgctgatc gtccggaaat
     1261 accccgggag cttttccgtt ttcctgaata cgaccgcgct cgcgttgggg atcgtgtttt
     1321 tcacgaacag catctcacgc acttcacggg ccgcatcgac ggaaagttcc tgcttccatc
     1381 gttcgggaaa acgttcgtcg agccacgggt ttgtttcacc ggccagcgtc ccgtcagcat
     1441 ccacgacaag gggaagcccc atgaaaagat cagcgcctcc ttccagccgg ttcacggcga
     1501 tcgccaggaa atccgtttcg cagaaatcgt cgctctcggc tatccagata tattttcccg
     1561 aagcgagcga aattcctttt tcccattgga gaaatgtgct cccgctgttc ttttcgttat
     1621 aaacgatatg cgtgaccttc gcgtgaccgc ggaaacgttc gaggatcgat acgcttatat
     1681 cggacgaatg atcatcgagc aggataattt cgaaatcggt gtacgtctgc gcgaagatgc
     1741 tttccatccg cctgtccaga aatgcggcgt ggttgtaatt gggaacgata acggaaacaa
     1801 gcggattcat gcaggcgtat ttcagcggtg aggcggaaca atggtggcga agcccggtgc
     1861 aaatgcattc gcccgttccc gtgaacgaac cccggtatat tcagaatgct gcatcaggcg
     1921 gtcaacgctt tttgtgtttc ttccaggtaa gccctgcccc atttcctgca tggctcaaga
     1981 tggtttcctt cggcccattc attccatgcg ttgataaaaa taaaattttc atccacgctg
     2041 taaggcttga aaccggcgat gatgtgccgc agccatttgc catattcccc cggggtggaa
     2101 tcatgtatga tcgtggcgcc cgtgcttcgt ctcggtgaat tatcccacga ggggaaaatg
     2161 ccggggtagc gtttaaaggc aacggccggt tttctgagca tttcgcggac cacatctttg
     2221 tagaggaaga tattgtcgcc ggttgctttt gcgcccgcca ggcgcgacca gagcgatctc
     2281 tgcggacgca ccggttgcgg caggaaagaa aaatcaggct gaaattccac ggcagcgtcg
     2341 aatcccagct ctgcaggatc acggcggtcg ctgaacgact gcatgtacat gaggtagatc
     2401 tcgcccactc cgttcttccg gcattcctcc cgccaggttt ccgccgttct ccgtatgtcc
     2461 gggaaaagct tcggccggta cacaatgaaa acaggttttc cgtcgacacg gatgtaacgc
     2521 ggatcacgaa aatagcgggc gaggaaattg atgtgcctgc ggtcatcctc ctccgaatag
     2581 tcctgcttca tcagcacttc ctgctcgagg ccgtcccacc gccgcgtcca gttctcgttc
     2641 gcccagcaaa gacaaaaagg gaaatccggc gttccttttg caagcatgtc ttccagcgga
     2701 cgttcgagca ggcgttttcc attgaaccag taatggtaaa agcagaaacc gtaaatgccg
     2761 tattgcttcg caagctccgc ctggctgatg agcgcctggt gcacgcgcag gtcgtaaaat
     2821 cccatatcgg cgggcaggtg gggctggtaa tgccctttga aacggggaac ggtcttcgtt
     2881 acgttcgtcc attcggtaaa tccttttccc caccattcgt cgttttcagc gaaaggatga
     2941 aactgcggca ggtagatcgc tatgggcctg agcttactca ccggcaaaaa cttttttcac
     3001 tgtttcaagg aaagctcttc cgtacctgcg gcagggctcg aggtgattcc cttccgccca
     3061 ctcgttccac gcattgataa aaacgaattg ctcttccccg ggcagcttca tagcaatttc
     3121 tttcgcccag cggagagaat cttcaaaggg ctgcgtgtcc tgtcccacaa gtatgatccc
     3181 cttctctcct ctccgcgcag tattatccca cccgacgaaa ggacagggaa caaatttttc
     3241 cggcaggcgg cgttttttca tcaggttttt tgtttctgcg tagctgtata atttcagcgt
     3301 tccgctgtac aatccgaaac gcgaaacatt gtggagcagc cgtctccacg accatttgtc
     3361 gtgaaaggcc tgctgcagca atcccagctg cggttcgaaa ttgagcacgg tatcgaacac
     3421 ttccggcttt ttcagcgtgt gtgaattgtt gccgatgagg taaagtcccg ggaagccttt
     3481 tttaacggcc gtctcccgga agattttcag cgtttcgccg atgtccggaa aatcattggg
     3541 gcggtagatc acgaacactg gttttccatc cacacggatg taacgcttgt cgggaaaaac
     3601 ccgcccgcaa agccattcgg cgtgatgcag gtcgtcttcc ctgctgtatt cctggcggat
     3661 gagcacatcg cgttcttcgc caagccatct cctcgaccag ctctcgttcg cccagaaaag
     3721 aaggaaagga aaatcgggac gtttggtttc gagcaaaaga tcgagcggat catgcagcaa
     3781 acgttttccg ctgaaccagt aatgataaaa gctgaacccg tgaatagcat attcccgcgc
     3841 cagcgccgcc tgctcctcga tcgtttcgat ggcgcgcaga tcgtaaaaac caagatccga
     3901 cggaaggcga ggctggtaat gatccctgaa cagcggcttc gctttggtca cgttcgtcca
     3961 ttccgtaaaa cctttgcccc accatttgtc attctccggt gtaggatgga actgtggaag
     4021 atagatcgcg atgggacgaa tcatattatt tctcctgcgg ctttctgctg atgaacactt
     4081 ccaggaattg tccgtccagc ccttttttcc ggtcggtctc caggtagatg gttgtcgtca
     4141 ttccgccgtg ctcaaaaata aaatcggtaa aatcgagccc ccagtcgaaa gtaaccagcg
     4201 caccttttcc tttgccaatg ggatttccgt gatagatctc ctccctgaag tattcaatgc
     4261 ttccgtcgct cctgagtcgt gcgcgctgca ccgatttttt atgggcgggc acccacggca
     4321 tggtgaatat gtgcatgccg cccggccgga gaacacgcgc cacttcgcgg aacgctttgt
     4381 caggctcgaa tacgtgctcg aaaacatcgg aggtgatcag caggtcgaag gaagcgtccg
     4441 ggaacgtcat ccgtgaaaga tcttcggagc ggtgaccgtt ccggtattcc ccgcgcggga
     4501 catcgtcgta ataatgcgag ctggtataat tgcggcagtt ctttttcagt agttccgaaa
     4561 cgcagccccc gggcgatgat tcgtggatat cgaggttcgg ccagtcctta cagtaaagat
     4621 ccagcacatt gacaagcgca cggttcctcg ggatggactg acagcgtttg cacctgtaat
     4681 tatcgcggag ccattcgttg taaataataa actcggtttc ctgccggcag atgcagcaat
     4741 acccgctatt cctctccagc cgggattcct ttacggccgc acggatgttc cgcggaagat
     4801 ccagcatctt tcttgcaatc cttttcagtg gcctgatcat ctcctgcctg ttttgaacaa
     4861 aatacaaaag tttatcacat gcattgtcgt ggttcagctg ccgagtctcc gtgcaaccct
     4921 gccgaaaaaa aaggatttcc atttctttcc cttcgcagca tattccgaag aaagtttccg
     4981 gaacctccag cctgccggcc tgaattcctt cctgaaaaca cgcagtccga aataccggta
     5041 caggttcgca ggcagctctt cgtaaaactt ttcctctttg cgcaccgcgc tcgttccccc
     5101 gccactgaat tcagccacga tcacatccat gtacaccagt gatgttccgg cggcgcagcg
     5161 gagaataaaa tcccagtcgg cgcacacttt atactccggg ttgaaatacc cgaacttttc
     5221 aaaaacatcc cggtgataaa acaccgactg ctggcaaatg tttgcgcgga gcagcttctc
     5281 cgttgtaaac tccccgtcat gaacttcgcc gtcttttgcc cagcctgcat ctccttttat
     5341 tttcacgttt ccgtaaacga cgcctgaacc ggctttttcg agaaaagggg cgactttttc
     5401 cagcgtggtg ttttcgtaca acctgtcgtc gctgcccagc acatacatcc atcggccctt
     5461 cgccatccgg atgcctttgt tgatggcgtc atagatcccg tcgtcctttt ctgatttcca
     5521 ccggagcttt ttttcctttt cgtacgcgcg aaggatatcc actgtttcgt cggaagactg
     5581 gccgtcgacg acgatgactt ccacgttctg cagcgtttgc ccgaacacac tgtccaggca
     5641 cggacgtatg gcaggaccgg aattgtacgc aggtacgatg acagaaatga gcggttcact
     5701 catgctcaca ggcaggtttc gtttccggga ggcggtgtga tcgcaaaatc gccgaggcgg
     5761 aaagcggcat tcttccggtc gtaggaaatc gggagcggga acccgaaaag cgatttatat
     5821 tcctgcagac gttttttgcg caggaacgcc gggggcgatt gcctctcatg gtaccgccgg
     5881 atctttttca gcgttttctt ttcttcttcg ctcagtcgtc ccaggcgggc cgcaagcgcc
     5941 gcgttcgctt cgcggttgta atcatccatc ttttcgagcg tgaactggtt ctttgtccgg
     6001 aaacgaaaag cgccgagcgt cgtgcccacg ctgtgcagct gcgcgtgatc gaaaaaacgc
     6061 agccataatt cgtaatcgcc ggcataacgc agcgacgtgt cgagtctcgc ccctgctttt
     6121 tcccaaaggc ttctcctcca gaaagtggat tcctgctgga tgtgctccgg gtcgccgagg
     6181 aaaaaattat acttcgacca tttgcggaaa tttttgacca tcaccgtgcg cccggtttca
     6241 tcgataacgg aagccattcc ctggatccat tccacctgcg gcatcgcgga aaagatctca
     6301 cccgccaccc aaagcgagcc cgggtgaaaa aggtcgtcgg aattgatcca tgccatcact
     6361 tcccccgttg cacgcgacaa tcctttctgg aggccgtcgt acatcccgtt gtctttttcg
     6421 ctgatccaga aggcgaggtg cttctcgtat ttgcgaatga tgtcaacgga accgtctgtg
     6481 cttcccccgt cgatgatgat atattcgagc gcaggatacg cctggtcaag aagggaacgt
     6541 atcgtgcttt ccaggaatcc cgcctggtta taggaaacag ttacgatcga aattttcggg
     6601 tactgcataa gataaagata agcctaagtc ttcacgtcgc ttcccgtggc ggccgcggga
     6661 aagccgggaa tcctctcatt ttgcccggct ccatttccgg cggaacgcat cgatctcgct
     6721 ttcggtgtaa aaatgacgaa cgtatttcga attatacaaa gcgtcaagct ttccgggaga
     6781 gggctcgtac tgcgacatca gttcggagta cagctgcttt ccttccttcg attccgatgt
     6841 attcgacgag cgcacaggca cgatgtcgag gtgcgtgaat tcgcgcatcg ctttccggaa
     6901 acaggtgctc atggcctccg ttttcatgca gagaatatcc gctttgcctg tgtggtaaat
     6961 actgtagcct ttctccttgt tgaacggcac cgaataaata tcgaaaccgg tccaggcttt
     7021 gaactccgta tcaaaccagt taagaacata ttcgtgatcg tgggcatcga ggtactcttt
     7081 cagccgggaa aacgtaagat cattcacgga agaaacaccg aacagtcctt tccagttctg
     7141 aaaaatatcc gatatctcgc gcgacatcgg ctcgcgcacc atcgtgatga tctggatgcg
     7201 tttgccgggt tgttccttca gcgcctgctg cacctgccgg gcgacttcaa tattcacatg
     7261 aaaagcggga tcaagcgacg gcagcttctg gttgacccag aaatccgaca tgaaatgaag
     7321 atggtaaacg ggaagatccg ggtgctgcgt cttcaggtgc gaatagacgg ttgtcgttcc
     7381 cacttttccc atggaataga ccaggaaaat tttttttccg tgccgccgcc ggtacgcaag
     7441 cctgcgcgca ggtgccgtaa atgtacgtgc acgcctgacc agggaagacc gcactctgcg
     7501 caaaaaatca ctgatcatgg ttgcggaaaa agattcggaa acaaatatac agtatcaggc
     7561 aaaggtgttt ttggcgaagg cgggatgatc tttcctgagg aagaaagcat cgcactgcca
     7621 gaacatcccg tcacccggcc ttcgcacggg atggatcacg tcagaaagac ggaagccgag
     7681 cttttccatg tgaatgcaca tttcaggaaa aagaaggcaa ttcgccgcaa tacggaaacc
     7741 gtagcattcc atgatgataa ggtcagtctt cttcagcgtt tcgcgcgctc cttcgagaat
     7801 cggtatttcg aaaccatgcg tgtcgaactt gatcacgaat ggtcctgcaa gacccaaacg
     7861 ttccacttca tgatcgatcg tggtcagctc cacctcgcgg tcgccgcctg tcgattccgc
     7921 atcgtaaacg ccgcttccgt caaggtcgcc ggcgaccacg aatttcactt ttcccacccg
     7981 gtcgcctgct gctgcgaaaa cagttctgaa tttcgggtgc gccgcggcga gcgcggacag
     8041 ctcttctttg cgctcttcca gcggttccat gagcaggaaa tacgcatccg gccagaaggt
     8101 gcaggctttc tccgtccagc ttccggcggc agcgccaagg tcgatgatgg cagacggttc
     8161 gacgccgtgc atctttttcg cacgcgacat gcccgcctcc atgctgtcct cgtccctttt
     8221 tgtcctcgcg acctgcggtt gcaccgccgg cgttccgctg cgacgtactt cgagtccgag
     8281 catcctgaac aaagctttca gtacattctt catttttcct gttctttttt cctgcgcgaa
     8341 tccgcatcca ttaatgaatg aacaatatcg ccgaatgttt ccgcaaaacc tttcagccgg
     8401 agcggcaaag tttcccgtgc gtgtttttcc atttccgcgt cagggccggc ggccgagctg
     8461 ttccacgcgg agagcatgag ctccgccagt tcaccggcgt tatccggatc gaaatacgat
     8521 gcgccgggag gatcctgttc cctgtgcacg tccagcgatg aaagcaatat cttcttcccg
     8581 atcgatttca cctcttccac ggtcgtgctc catccttcga agcgggaagc gttgatcacg
     8641 aaacagctct gccggatcag ctgcgccacg tcttcttttt cgatgatccc aaggacggaa
     8701 atattttttt cgagcccgtt cgcctttacc aggccaagca gcgaatcata atgtccgggg
     8761 ttgcgcggat cgagcgtgct gcccgaaagc accacatgca gctcatggtt ccgccgcttc
     8821 aggacagcga gcgcttcaat gatcagcgca tgatttttat gcacccagaa ctggttgggg
     8881 acatagatga atttttcagg cagatggtat ttctccagca ggaaacgcgg atccttttca
     8941 tacaccgcgc cgggaatatc aacgacaaaa cgaagcactt tcactttgct ttcatgcccg
     9001 gggtaaaaac ggttgagatc tttccgggcc gcatcgctgc tgagcaggat aatgtcggcc
     9061 tttgctgcac ccgcggcgta attttttttc ctgcgttcga tgtcttccgg tttgaaatac
     9121 ccgggaagat ggaacacctg gaaatcgggc acccagaaaa tcacgggaag atccgccagc
     9181 atcgggaaat gacctgaaaa aagtacgaat gcgcatattt cccttccggg tgaaagcgat
     9241 tcgaggtatt gccgccgccc acgcgaaacg aaagacggtt cttccttccg gcgcagcaac
     9301 ctgttcatcg ccacccggaa tttatcccgg aacgcagggt aaaaaacttt tacatccgtg
     9361 tagctgatcc cgcgaagttc aacgtccgta agaaagcggg cggtcacctc ttcgagattc
     9421 ctgaaataat tggcgccgcc gagccacgtt tccgcttcaa tccggtgata aaaaacagtg
     9481 attcccgttc gccccatagg tttcaaaaat acaaatctgg ttcaacagcc ggcgacgcgg
     9541 ttttccggat cacacagagc ggtaatcctt cacggtcagc gcaagtcctt ccgcaatggt
     9601 taccgccggt ttaaagccga gtcccgaaat gcgggaaata tccgcctgcc agtttttcgg
     9661 atcgccggcc ttgacctcac cgttgaacga aagctggaat cctcctccga gggaatcaag
     9721 gaaaacggaa gcggcttcgc ggatcgttgt ttccactccc gaagcgacgt tgatgcattc
     9781 acccgcgaaa gctccattct ccatcaccgt ttccagcgca cgcacgagat cgcggatgta
     9841 aataaaatcg cggctttcct cgcctgtccc gaaaagctcc agttttttcg actgctttgt
     9901 cttctggaaa atatcccaga aaagctgctt gtgaagtcgc ggaccataaa cggaaaaaac
     9961 gcgcaggctt accgtgcgaa ggtcgtgctc ccggtgaaag gtttccagca cctgctctgc
    10021 ttccagcttg tgttttccgt aaggagaaag cggcgccggc attgcatctt cggagaccgg
    10081 gagacgggat ggatttccgt ataccgcagc gctcgacata ttgatgaacc ggcatgcggg
    10141 ctgatgcgcg cggatcgccc cgagcatgag gcgcacattc tgtgtattca gttcataatc
    10201 tttttccggt gacgcgagtg aataaccgac gccgggtgat ccgcttgcat tgacacagca
    10261 atcgaacggc acggaagaaa aaatcctttc atacgttccc ggcccggaat agcggaaaaa
    10321 gcgttcatca tccgcacctg taacatcaca accata
//
LOCUS       DASTSF010000289         2538 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_148060,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000289 DASTSF010000000
VERSION     DASTSF010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2538
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148060"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            306..>2538
                     /locus_tag="VFU15_08455"
     CDS             306..>2538
                     /locus_tag="VFU15_08455"
                     /inference="COORDINATES: protein motif:HMM:NF024962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprB repeat-containing protein"
                     /protein_id="HEU4717852.1"
                     /translation="MKKLVLFPIVLLLGFLLVLSRQAHAQASACPAVTTGTVAPVCSG
                     QCVNLTATVQGTLQTNTYTVSGIPYSPYPYTGGNSVLINIDDTWSPVINMPFCFQFFG
                     NTYNQIVIGSNAIVTFDVTQANGYCQWPINNAIPSNANPMNSIMAPWHDIDPSVGTPT
                     SATDVNWQVFGSAPCREFVISWNDVAMFDCNNLIATSQLVLHESTNIIDIYIQNKPLC
                     STWNGGAAIEGIQDATGSTAFVVPGRNYPTQWTASNDGQRFMPAGTPNYTLQWTGPSG
                     PVGTTATVNVCPTATTTYTCTVTNTSCSGPIVVSATETVTVGGGITTSGTQSNSSSCT
                     ACNGSATVSITSGTGPFSYSWSPTGGSAATASGLCPGTYTCTISNTAGCNTTQTFSIT
                     GPSNPTSIQSSTNVSCNGGCNGTGTITPSPAGAYTYAWSPTGGSAATASGLCAGTYTV
                     TATNAGGCTTTQTITITQPPALTTTSSQTNVTCNGGNNGSATVNPSGGTGPYSYSWAP
                     NGGTGATANGLTAQGYTCTITDAAGCTITQAFNITQPSPVTASVSTTPSTCSGNNGTA
                     TVNPSGGTGPYTYSWAPAGGTGVTASSLGAGSYTCTVTDAAGCTVTATGNVASTGGIT
                     ATVTATTNVSCNGGSNGSATAAPTGGVGPYTYSWSPSGGNGTTASGLGAGSYTCTVTD
                     ANGCIATTVATITEPTALSLTSSQVDELCNGGNTGSATVNISGGTSPYSYSWSPSGGN
                     GATA"
BASE COUNT          597 a          723 c          686 g          532 t
ORIGIN      
        1 cagcctcgtc aggtgatctt tttttgtttt tgttttaaaa ccggctccga gggaatgccg
       61 gttttttttg tgtgaatgaa aggcgatgca ggaatttctt agccctgatt gaaaagcagg
      121 aagagagaga atgaaacgca caggtgatgc gctccgtgtt ttttccggaa aagacagacg
      181 agttgcttta tgaaattatg caataagaat tgataagaaa agcattagat tttttcatta
      241 tttccctgtt ttttctaatt tagttaacgg caaacagaat tggttctctc accaaacgat
      301 ttcgcatgaa aaaattagta ctctttccca ttgttctgct attgggcttt ctgctggttt
      361 tgtcccggca agcgcatgcg caggcatcgg cctgcccggc ggtgacgacc gggaccgtgg
      421 ccccggtctg ctcaggtcag tgcgtcaacc ttacggcgac ggtgcaggga acattgcaga
      481 cgaacacgta cacggtttca ggcatcccgt acagtccgta cccgtacacg gggggaaatt
      541 cggtcctcat caatatcgat gatacgtggt cgcctgtcat caacatgccg ttctgcttcc
      601 agtttttcgg aaacacgtac aaccagatcg tcattggttc caatgcaatt gtgacgttcg
      661 atgtgaccca ggccaacggc tattgccagt ggccgatcaa caacgcgatc ccttcgaacg
      721 ccaacccgat gaattccatc atggcgccct ggcatgatat cgatccctcg gtgggaacgc
      781 ctacttcggc cacggatgtg aactggcagg ttttcggaag cgccccgtgc cgtgagttcg
      841 tgatcagctg gaatgatgtg gcgatgttcg actgcaacaa cctcatcgcg acatcgcagc
      901 ttgtcctcca cgaatcaacg aacatcatcg atatttatat ccagaacaag ccgctgtgtt
      961 caacctggaa cggaggagcc gccatcgaag ggatacagga tgcaacgggt tccaccgcgt
     1021 ttgtcgtgcc ggggagaaat taccctacgc aatggaccgc ctcgaacgac gggcaacgat
     1081 ttatgccggc tggtacgcca aactatacat tgcaatggac cgggccaagc gggcccgtgg
     1141 ggacgacggc cacggtgaat gtctgtccga cagccaccac aacatacaca tgcaccgtta
     1201 ccaacacttc gtgttccgga ccgatcgtgg tttccgccac tgaaacggta acggtgggag
     1261 gcggcatcac cacgagcgga acacaaagca actcctcatc gtgcacggcc tgtaacggtt
     1321 cggcgacagt ttccatcacc agtggcacgg ggccattttc atattcgtgg tctccgacgg
     1381 gaggctcggc cgcaacggcg tccggtttat gtccgggaac atacacctgc accatcagca
     1441 acacggcggg gtgcaacact acgcaaacat ttagtataac cggtccgtcc aacccgactt
     1501 ccatccagtc gtccacgaac gtttcgtgca acggcggttg caacgggacc ggaaccatca
     1561 cgccatcacc ggcaggggct tacacatacg cgtggtcacc gaccggcgga agcgcggcaa
     1621 cggccagcgg tttgtgtgca ggaacttata cagtcactgc tacgaatgca ggcggatgta
     1681 cgaccacgca gaccattacc atcacgcaac ctccggcgct cactacaacc tcttcacaga
     1741 caaacgttac ctgcaacggg ggcaataacg gttctgccac ggtgaatcct tcgggcggaa
     1801 cgggaccgta cagttattcc tgggcgccga acggtggaac aggagccacg gcaaacgggt
     1861 tgacggcaca gggttacacc tgcaccatca ccgatgctgc cggttgtact ataacacaag
     1921 cgttcaacat tacgcagccc agcccggtaa cggcttccgt ttccacgaca ccctccacct
     1981 gcagcggcaa taacggaacc gcaacggtga atccttccgg cggaaccggg ccttacacgt
     2041 attcctgggc accggccggc ggaacaggtg ttactgcttc ttcgcttggc gcaggcagct
     2101 atacctgtac ggtaactgat gccgcgggct gtacggtaac tgcaaccggc aacgttgcga
     2161 gcacaggcgg aattacagca acggtgacag caaccaccaa cgtctcctgt aacggcggca
     2221 gcaacggatc tgcaacggct gcaccgaccg gcggcgttgg gccctatacc tattcatggt
     2281 cgccgagcgg cggaaacggt acaacagcga gcggacttgg cgcaggttcc tacacctgca
     2341 cggtaacgga tgcgaacggg tgcattgcga caaccgtggc gacgatcacc gaacccactg
     2401 cgctatcgct tacttcgagc caggtggatg aattgtgcaa cggaggcaac acgggatctg
     2461 caacggtgaa tatttccggc ggcacatcgc cttattcata ttcgtggtcg ccgagcggcg
     2521 gaaacggtgc gaccgctt
//
LOCUS       DASTSF010000290         2376 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1210522,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000290 DASTSF010000000
VERSION     DASTSF010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2376
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1210522"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            305..1471
                     /locus_tag="VFU15_08460"
     CDS             305..1471
                     /locus_tag="VFU15_08460"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU4717853.1"
                     /translation="MRYLSADHVFSAHTGFIPNGILVLNDDGTVSDLLDPRHAADIPD
                     AERLDGTICPGFINAHCHLELSHLKGKIRKNTGFVGFAKELIPMRGKFSAEEIARAVA
                     EAEEEMYTNGIVAVGDISNTADTVAQKKKGTLRYHTFVELLGLKAMFAEPVCETGREL
                     LKKFPEPASLAPHAPYSVSESLLKLIAADCEAAKRITTIHNQESGDENEFFISGKGKM
                     KELYAFLKMDISYFNPPGVNSLRRTLPQLPGKQNLVLVHNTFTSAEDFQWANSTRENI
                     FWCLCPNANLYIENTLPDFSVFTANGAKIVVGTDSYASNEKLSVFSELQVIHEKAPGI
                     GVETLLTWATQNGAEALRMNELGSFEADKKPGVLLLKGVSPEKISAEAAVKRLV"
     gene            complement(1566..2282)
                     /locus_tag="VFU15_08465"
     CDS             complement(1566..2282)
                     /locus_tag="VFU15_08465"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014680376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEU4717854.1"
                     /translation="MLKRYVIIVAAGKGSRMQAPVPKQFLRLAGKPVLLHSILAFHQF
                     DPMAKIILVLPGDQLQQWEKLVKEFDVLIPCEITGGGDTRFESVKNGLALIPENENAI
                     VAIHDAVRPLVAQKTISASFKAAERYGNAVPAIPLNDSIRQIESSRSVAVDRSKYCLI
                     QTPQCFEVGLLKKAYAQEYKFTFTDDAGVIEALGEQIHLVDGNPDNFKLTTPTDLVLA
                     EAILKMRSQFVPDVSGGTHP"
BASE COUNT          562 a          586 c          668 g          560 t
ORIGIN      
        1 ctgctcagcg tgtccagtgt acagccccag gatttcaggc tgatcgatac agccagtgaa
       61 gcgaacgcca ctgtgaagca cagtgcaagc gctacggttt tcgtacggat tgtttttttc
      121 atgttttgtg tagttggttt aaatattaat aaatgaatta tttattagtg aatttataaa
      181 atattatata tatttataat aaataaataa tttatttttt acttgtttat ataaaatgtg
      241 aaaggagata ttgcccggat cccctcccgc cgtgtattgt gggaatgcgg taatttcgtc
      301 tttgatgcgt tacctctccg ccgatcacgt tttctccgcc catactggtt tcattccgaa
      361 tggtatcctc gtcctcaatg acgacggtac tgtcagcgat ctgctcgatc cgcgccatgc
      421 ggcggatatc ccggatgcgg aaaggctgga cggaacgatt tgccccggct tcatcaatgc
      481 gcattgccac ctcgagcttt cacacctgaa aggaaagatc aggaagaaca cgggcttcgt
      541 cggttttgcg aaagagctta ttcccatgcg cgggaagttc agcgcggaag agatcgcacg
      601 cgcggtcgcg gaagcggagg aagaaatgta tacgaacggg atcgtcgcgg tgggagatat
      661 ttcgaatacc gccgacacgg tggcgcagaa gaaaaaggga acgctgcgtt atcacacgtt
      721 cgtcgaattg ctcggactga aagcgatgtt cgcggaaccc gtttgcgaaa ccggccgcga
      781 gctgctgaaa aaattcccgg agccggcgtc tcttgcgccg catgcaccgt attcggtttc
      841 ggaatcattg ctgaagctca tcgctgcaga ttgcgaagcg gcaaaacgca tcaccacgat
      901 ccacaaccag gaaagcgggg acgagaatga attttttatc agcgggaaag ggaaaatgaa
      961 agagctgtac gctttcctca agatggacat ttcgtatttc aatccgccgg gcgtgaattc
     1021 gcttcgccgg acgttgcccc agcttcccgg gaagcagaac ctggttcttg ttcacaatac
     1081 gttcacttcc gccgaagatt ttcaatgggc gaattccacc cgcgaaaata ttttctggtg
     1141 cctgtgcccg aacgcgaacc tctacatcga aaatacgctg cccgattttt ccgtgttcac
     1201 ggcgaacggc gcgaagatcg ttgtgggcac cgacagctac gcgtccaatg aaaaactctc
     1261 cgttttttcc gagcttcagg tgatccacga aaaagcgccg ggcatcggcg tcgaaacatt
     1321 gctcacgtgg gcgacgcaga acggggcgga agcgctgcgg atgaatgagc tcggctcgtt
     1381 cgaagcggat aagaaaccgg gcgtgctgct gctcaaaggc gtttcgccgg aaaaaatttc
     1441 tgcggaggca gcggtgaaaa gactggtgtg aaccccggtg cccgtgatgc gggtgttttg
     1501 cctggccggc aggcagaatt tctcaggcgc agaagcggcg ctttattccg gaagcaaagg
     1561 aaagatcagg gatgcgttcc cccgctgacg tcgggaacga actgcgagcg cattttcagg
     1621 atggcttcgg ccagcacaag atcggtcggc gtggtcagct tgaaattatc cgggttgccg
     1681 tcgacgagat ggatctgttc gcccagcgct tcgataacgc cggcgtcgtc ggtgaacgtg
     1741 aatttgtatt cctgcgcgta cgcttttttc agcagcccga cttcgaaaca ctgcggcgtc
     1801 tggatcaggc aatatttcga gcggtcgacg gctacgcttc tcgacgattc gatctggcgg
     1861 atggaatcat tcagcgggat ggcgggaaca gcgttgccgt aacgttccgc cgctttgaaa
     1921 gaagcgctga tggttttctg cgccacgagc gggcgaacgg cgtcgtgtat ggcaacgatc
     1981 gcgttttcat tttcggggat cagcgcaaga ccgttcttca cggattcaaa acgcgtgtca
     2041 ccgcccccgg tgatctcgca gggaataaga acgtcgaact ctttcacgag cttttcccat
     2101 tgctgcagct ggtcgccggg aagaacaaga atgatcttcg ccatggggtc gaactggtgg
     2161 aacgcaagaa tgctgtgaag aagaaccggt ttccctgcga gacggagaaa ctgtttcggg
     2221 accggggcct gcatccggga accttttccg gcagcgacga tgatgacgta ccttttcagc
     2281 atgaaagcgc gcgaaagtta agcaccctgc gcgtaggttg aatttcagct gcgaggtttt
     2341 ttttcacagc aaaaagataa aacgggatga aattct
//
LOCUS       DASTSF010000291         3752 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1471464,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000291 DASTSF010000000
VERSION     DASTSF010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3752
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1471464"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..615)
                     /locus_tag="VFU15_08470"
     CDS             complement(<1..615)
                     /locus_tag="VFU15_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014682542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase
                     N-terminal domain-containing protein"
                     /protein_id="HEU4717855.1"
                     /translation="MSHSNGHNEFKAGKLLSRIHTPQDLRQLAENQLPEVSKELRQYI
                     IDLVSKKGGHFGASLGVVELTVALHYVFNTPYDQLVWDVGHQAYGHKILTGRREVFHT
                     NRIYKGISGFPKRSESEYDAFGVGHSSTSISAALGMAVAANRKQEKDRNVIAVIGDGA
                     MTAGLAFEGLNHAGIEKSNLLVVLNDNCMSIDPNVGALKDYLNDI"
     gene            complement(735..2033)
                     /locus_tag="VFU15_08475"
     CDS             complement(735..2033)
                     /locus_tag="VFU15_08475"
                     /inference="COORDINATES: protein motif:HMM:NF025092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEU4717856.1"
                     /translation="MKFFITCMLLFLAAGAVSAQEKTFFVCGHITNTDKQAVPFAGVQ
                     VAGRSSGTNADRNGFYILRTALPAIVKVSALGYKTQQRTVNAIAGADTIRADFILISE
                     PVQLQPVDISSVHAAEKITEAVNLTDFELRDHLLWLLYSTANNNSLIAVTDTSGKNIH
                     RFPLAFCAEGISRTQHGYVYAISGDTVYLFDHNATGFTDFTMPVKTFSEQVLPLVAWK
                     DSVYYYEKKDTSQAMFSFWYFDKKHHITGTLYHFLDYEKYEANQETNYEINRINGTLS
                     DNEKNGMMSENGAPHGDFSRMIALTTMIQPVYCPLRLIRDSVYIFNFDDDSIYVYGKD
                     NRFVRRTNLAFDHYGLQYKNKDILVDEEGRSAYFKYTIKSVTYLEKINLDNGTKISAQ
                     SLDKFPFIEKIRISNGSAYFCLFDHSGPGKSRTLYKEKIN"
     gene            complement(2133..>3752)
                     /locus_tag="VFU15_08480"
     CDS             complement(2133..>3752)
                     /locus_tag="VFU15_08480"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEU4717857.1"
                     /translation="MPIGTDQSKTNEVTKQVEARINRLLGNDTDIVTSVIANVAFGAG
                     DPRANDLSIQPHKAKVTVAFKQFAGRHGKSTRAILERVQNSFHDNPIPGAQIVVEQEQ
                     GGPPVGKPISIEITGDNLAALPVVADSLRSFLDTLSIAGVQKLKSDVQTSKPELLFDI
                     DRERANREGIYSSQVANEIASGVLGPEVSKFRDENDEYPIVVRYKKDQRSDVNTIQNL
                     KLTYRDMTMGGQIRNVPLSSFARVVDTVSFGGIKRKGQKPIITLESDVISGYNPNEVA
                     ANVMTQVEYWKEHHKGMLAGISVSMGGQQEEQAETASFLGMALLASIGLIIIILVTQF
                     NSVGKPIIILSEILFSVIGVFLGLAIFGMNVSIVMTGVGIVALAGIVVRNGILIVEFI
                     DILKERGLPTREAIIEAGKTRMTPVLLTATATILGLIPLAVGLNIDFGSLFTELNPHI
                     FFGGDSVAFWGPLSWTMIYGLSFATVLTLIVLPVMYLLSENAGKKLKKLSGSAADEAA
                     HHAHMYGTMEPVLAGDKVSHDILPDDDHRSN"
BASE COUNT          812 a          956 c         1046 g          938 t
ORIGIN      
        1 gatgtcgttg aggtaatctt tcagcgcccc gacattcgga tcgatgctca tgcagttatc
       61 attgagcacg acgagcaggt tgcttttttc aatgccggca tgattgagtc cttcgaatgc
      121 aagtcctgcg gtcatcgcac cgtcgccgat cacggcaatg acgttgcggt ctttttcctg
      181 cttgcggttg gcggcaacgg ccattcccag cgcagcagag atggaagtgg atgaatgtcc
      241 cacgccgaat gcatcgtatt cgctttcgga ccgtttcggg aatcccgaaa tgcctttgta
      301 aatgcggttc gtgtgaaaaa cttcacgccg gccggtgaga attttgtgac cgtatgcctg
      361 gtgacccaca tcccacacga gttgatcgta cggcgtattg aaaacatagt gcagcgccac
      421 cgtcagttcc acgacgccga ggcttgcacc gaagtggccg ccttttttgg aaacgaggtc
      481 gatgatgtat tgacgcagtt ccttgcttac ttcaggcagc tggttttcag caagttgtcg
      541 taagtcctgg ggcgtgtgta tccgcgaaag aagttttccg gctttgaatt cgttatggcc
      601 gttggagtgg ctcatttcag gtcaatgcaa aaatacgaag aattggatgc gttccgacag
      661 gcagaaaacg tggctttcaa cggtttcgtg tggatatacg aaagtgaagc ggttaaggat
      721 tgttaaggcg aagctcagtt gattttctct ttgtacagcg tcctgctttt gcccgggccg
      781 ctgtgatcga acaggcagaa atacgcgctc ccgttgctga tgcggatctt ttcgatgaaa
      841 ggaaatttgt cgagcgactg cgcactgatc ttcgtgccgt tgtcgaggtt tattttttca
      901 aggtaagtaa cagactttat cgtgtatttg aaataggccg agcgtccttc ttcatcgaca
      961 aggatgtctt tgtttttgta ctgcagcccg taatgatcga aggcaaggtt cgttcttcgc
     1021 acgaaacgat tgtctttccc gtaaacgtaa atggaatcgt catcgaaatt gaaaatgtaa
     1081 acgctgtcgc ggataagacg cagcgggcag tacaccggct gtatcatggt cgtcagtgcg
     1141 atcatgcgtg aaaaatcccc gtgcggggcg ccgttttcgc tcatcatccc gttcttttca
     1201 ttgtccgata acgtgccgtt gatgcggttg atctcgtaat tcgtttcctg gttggcttcg
     1261 tatttttcat aatcgagaaa atgataaagc gttccggtga tgtggtgctt tttatcgaaa
     1321 taccagaacg agaacatggc ctgagatgtg tctttcttct cgtaatagta aacggaatct
     1381 ttccacgcca cgagcgggag aacctgctcc gaaaaagtct tcacgggcat cgtaaaatcc
     1441 gtgaacccgg ttgcgttatg atcgaaaaga taaaccgtgt cgccgctgat cgcataaaca
     1501 tacccgtgct gcgtgcgcga aatgccttcg gcgcaaaagg caagcgggaa acgatgaatg
     1561 ttcttccctg acgtgtccgt tacagcgatg agactgttgt tgtttgccgt cgaatacaac
     1621 agccacagca ggtggtcgcg gagctcgaag tcggtgagat tgacggcttc ggtgattttt
     1681 tccgcagcgt gaacggaaga aatatccacc ggctgcagct gcacaggttc agaaataaga
     1741 atgaagtcgg cgcggatcgt atctgcgccc gcgatggcat tcaccgtgcg ctgctgcgtt
     1801 ttgtaaccga gcgcggaaac tttcacgatc gccggtaatg ccgtgcgcag gatgtaaaat
     1861 ccgttccggt ctgcattcgt gccggaagat ctcccggcga cctgcacgcc tgcgaacgga
     1921 accgcctgct tatcggtgtt cgtaatgtgc ccgcaaacga agaatgtttt ttcctgcgcg
     1981 gaaacagctc ccgccgccag gaacagcagc atgcacgtga tgaaaaattt catgcggtca
     2041 ggagcgaagc tacttcaaac ttaccgttat tgaaaagacg aacgttccgc gcaattccat
     2101 aaaaaaaccc gggcggaccc gggtttctgt ggttaattcg agcgatgatc atcgtccggc
     2161 aggatgtcgt gcgatacttt atccccggcc agcacaggtt ccattgttcc atacatgtgt
     2221 gcatgatgcg cggcttcgtc cgctgcagac ccggaaagct tcttcagctt tttgcctgca
     2281 ttctcggaaa gaaggtacat caccggcagc acgatcagcg tcagcacggt ggcgaagctg
     2341 agcccgtaga tcattgtcca ggaaagcggt ccccagaacg caacgctgtc gccgccgaag
     2401 aaaatgtgcg ggttcagttc cgtgaacagc gaaccgaaat cgatgttgag cccgaccgca
     2461 agcggaataa gtccgaggat cgttgccgtt gcggtaagga gcactggtgt catccttgtt
     2521 tttcccgctt cgatgatcgc ttcacgcgtg ggaagtccgc gttccttcag gatgtcgatg
     2581 aattcgacaa tgaggatccc gttccgtacg acgatgccgg cgagcgcgac aattcccacg
     2641 cccgtcatga cgatggaaac gttcatgccg aagatggcga ggccgaggaa cacgccgatg
     2701 acgctgaaca ggatttcgct gagaatgatg atcggcttgc cgacggaatt gaactgcgtc
     2761 accaggatga tgatgatgag cccgatcgaa gcgaggagcg ccatgccgag gaaagatgcg
     2821 gtttccgcct gttcctcctg ctggccaccc atgctgacgg aaattcccgc gagcattcct
     2881 ttgtggtgct ctttccagta ttccacctgc gtcatcacgt ttgccgcaac ttcattggga
     2941 ttgtacccgc tgatgacgtc cgactcgagc gtgatgatcg gcttttgtcc tttgcgtttg
     3001 atgccgccga agcttacggt atccaccacg cgtgcgaagg aagacagcgg cacattgcgg
     3061 atctgcccgc ccatcgtcat gtcgcggtac gtgagcttca ggttctgtat cgtgttcacg
     3121 tcggagcgct ggtctttttt gtagcgcacg acgatcgggt attcatcgtt ctcgtcgcgg
     3181 aacttggaga cttcgggtcc gagcacgccg ctcgcgatct cgttcgccac ctgagaggaa
     3241 taaattcctt cgcggttggc gcgttcgcgg tcgatgtcga agagaagttc cggtttgctc
     3301 gtctgcacat cgcttttcag tttctgcaca ccggcaatgg aaagcgtatc gaggaagctg
     3361 cgcaaactgt cggcgacaac aggaagcgcg gcaaggttgt cgcccgtgat ctcgatgctg
     3421 atcggtttcc cgacgggcgg tccgccctgt tcctgttcaa cgacgatctg cgcacccggg
     3481 atcgggttgt cgtggaaaga attctgcaca cgttcgagaa tggcgcgtgt tgattttccg
     3541 tgacggccgg cgaactgttt gaatgcaact gtcaccttcg ccttgtgcgg ctggatggaa
     3601 agatcgttcg cgcgcggatc acctgcaccg aaagcgacgt tggcgatcac cgacgtaacg
     3661 atatccgtat cgttcccgag aagacggttg atacgggctt ccacctgctt ggtcacttca
     3721 tttgttttcg actggtcggt accaatgggc at
//
LOCUS       DASTSF010000292         6926 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_134084,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000292 DASTSF010000000
VERSION     DASTSF010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 6926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6926
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134084"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1440)
                     /locus_tag="VFU15_08485"
     CDS             complement(<1..1440)
                     /locus_tag="VFU15_08485"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEU4717858.1"
                     /translation="MELQEQHKKNGYRELILPAAFALLFAVSLSPAFPSLRNSLLLFT
                     ELAAVAYMLIFRKGWLNGLLLFLVPLSVPVGADNGFVIDLPGEALTGLLAVSAFCAVL
                     FYPARFREIIRHPLVLIALAEIAWMVACSSFSADPLVSFKRSIVRMAYVSVFMILFSS
                     WLSEERNRSRFILLYAIGCIFPVINTEINHSTLGFLPLTAYWASRPFFNDHTLYGACL
                     AFLLPSLVIFSVRRKIFGLSGWKYAAVVLLTFFMCVAEWLSFSRAAWVSLVIAAVFAV
                     LVRARVRLWMVVSFLLVFVSLIFFNRDYLSGLAQRDDSVSSKGTITEHILSVANIQTD
                     ASNVERINRWNCAIRMAEERPLTGFGPGMYQFEYGRFQLKTDMTHISTYTGDRGHAHS
                     EFFNALSETGFPGLIIVVVIMFTAIGYGLRVIYHSKTEKERLVVLGVLLGLVSFYVHG
                     FFNAFLDTDKMAILVFGSMAVILATDISQR"
     gene            complement(1446..1808)
                     /locus_tag="VFU15_08490"
     CDS             complement(1446..1808)
                     /locus_tag="VFU15_08490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717859.1"
                     /translation="MADLRAKQPINLVVMPNSQYVIQAKTEAGTPAGTTQLERVMNEY
                     IFEQFRLNDISGRYEKAKTQYNRPVTLVFVVDRAKPSYRKAFPSLRVNLAIAGIVSLA
                     FSVFFFMFRERVTSLRKH"
     gene            complement(1766..2383)
                     /locus_tag="VFU15_08495"
     CDS             complement(1766..2383)
                     /locus_tag="VFU15_08495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717860.1"
                     /translation="MNENELKNEEGNEFLEFFSRKGKRIGIVVLVALIAATALTFILP
                     KEYRSQGIVFPANNNSVESIVDNPTVGYDLEADRLMQLLQSDAMFDSVQRHFHLADYF
                     ELDTAEPDWRDVLHLDYLKIVKFERTQYMSIVISAQTKKPELSAAIVNFIIDISDGMR
                     NNLYKRNQLAAYRSVEKNTSRKKTSSIPSKSWWRTSGQSSRSTLW"
     gene            complement(2545..3387)
                     /locus_tag="VFU15_08500"
     CDS             complement(2545..3387)
                     /locus_tag="VFU15_08500"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEU4717861.1"
                     /translation="MKKSTSSKLKSYTALAGSMTAVAITAHGQIIYTDINPDTIVHDS
                     ITYDLDMDNNATPELQFKVGVSTTPPNYPFVYASVVLNSPSTNAIISSVQGNYAYPMR
                     FNSGDTIQPSSPSWVDYATHPSQYLGSVYGSALYGNFRGQGDKFMGVRFDIGGQNHYG
                     WVRLYVSAGADTIIIRDYAYEAIADSAIIAGQTSSPLGISAGGNDGNIRVHSFENSIF
                     VHTGAQNPDGKVTVYNSTGQVVREEEVTAADMHISMEGQATGVYFVRVVDGTTAVTRK
                     VYIR"
     gene            complement(3581..6136)
                     /locus_tag="VFU15_08505"
     CDS             complement(3581..6136)
                     /locus_tag="VFU15_08505"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEU4717862.1"
                     /translation="MAERIAKRVLLIGWDAADWKIINPLLESGQMPALQKLISRGVMG
                     NIATLDPPLSPMLWTSISTGKTADKHGILGFVEPDPVTGKVRPSSVTSRKVKALWNIL
                     QQNGLRSHAVGWWPSHPAEPLDGVYVSNFFAKPNDAFDQPWPVAENSVHPGELAETLA
                     ECRVHPGEISFAHILPFVPDAHKVDQDKDKRLSGIATLLASGSTYHAVATWIMQNREW
                     DFLAVYLNEIDLFSHTFMKFHPPQMAGMPDELFALYKDVLNGAYRFHDMMLERLMELA
                     GDDTVIILTSDHGFHSENLRPKYLPTDPAAPAMEHAPYGVLCISGPGITPDERVYGAT
                     LLDIAPTVLTLFGLPVGKDMDGKALVQVLEKNVLPEFIESWENVSGNSGQHSSEVRED
                     PWAAQQAMQQLVELGYVEALGEDDAESADKIRRESRFYLARVFISTNRLDEAIPVLEE
                     LHREVPDVIRFGLRLAFVYHQAGRTEDARKIIDELRAKQERENDLPPLDYLEGSLLFS
                     EGRPLKALALLRRAEQGKSHMPGLHLLIGQIYTSTRNWADAERAFIRALSIDPDNARA
                     HHGLAFAALRQDKIELAIDEALNAVGLKYQLPAAHYHLGEALYKYGDHEKAAQAFEVC
                     VRMAPGNRRARMWLAKIYSEHLGENEKADAHIRFVKEFIRGTITVVSGLPRSGTSMMM
                     QMLRAGGLDVISDEKREADESNPKGYFEDERVKKLHLDNSWLADAEGKVIKVVAPLLQ
                     FLPAGYNYRIIFMQREIGEVLKSQQKMLGKKDDKNYPTTLADAFSKQLEKAQAWMKAQ
                     PYVSFLEVNYRDVIADPLAKAEEIAAFTGENPDPLAMAEAVSGELYRNRKSNV"
     gene            complement(6129..6305)
                     /locus_tag="VFU15_08510"
     CDS             complement(6129..6305)
                     /locus_tag="VFU15_08510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717863.1"
                     /translation="MEKKKDPVNEPAGKTYNIPADGSLGLLALGIDGLKAWRKKRREQ
                     REKSEGKTGRENNG"
     gene            6413..6607
                     /locus_tag="VFU15_08515"
     CDS             6413..6607
                     /locus_tag="VFU15_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007646460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HEU4717864.1"
                     /translation="MKTGTIKFFNATKGYGFIKEDGSGNEIFVHVTGLVDQPVNTGDK
                     VSFEVSEGRKGLNAINVKKI"
BASE COUNT         1568 a         1897 c         1877 g         1584 t
ORIGIN      
        1 ccgctgcgaa atatccgtcg caaggatcac cgccatgctc ccgaacacga ggatcgccat
       61 cttatccgta tcgaggaaag cgttgaagaa gccgtgcacg taaaacgaaa ccagcccgag
      121 cagcacgccg agcacgacca ggcgttcctt ttccgttttc gaatgataaa tgacacgcag
      181 gccgtagccg atcgcggtga acataatcac gacgacgatg atcagcccgg gaaaaccggt
      241 ttccgacaat gcgttgaaaa actccgaatg cgcatggccg cggtcacctg tgtatgtgct
      301 gatgtgcgtc atgtcggttt tcaattggaa acgcccgtac tcgaactggt acatgcccgg
      361 accaaaacct gtgagcggac gttcttccgc catccgtatc gcgcagttcc accggttgat
      421 gcgttccacg ttcgatgcgt cggtctggat gttcgccacc gaaagaatgt gctcggtgat
      481 cgttcccttg ctgctgacgg aatcgtcgcg ctgtgcaaga ccggacaaat aatcacggtt
      541 gaaaaaaatc agcgaaacaa aaacgagaag aaacgaaacc accatccaca ggcgcacacg
      601 cgcacggacc agcactgcga aaaccgcggc aatgaccagg ctcacccagg ccgcacgcga
      661 gaaggaaagc cattccgcga cgcacatgaa aaacgtgagc aggacaacgg cggcatattt
      721 ccagccgctc agtccgaaaa ttttccggcg cacgctgaag atcacgagtg aaggaagcag
      781 gaaagcaaga cacgcgccgt acagcgtgtg atcgttgaag aacgggcggc tcgcccagta
      841 cgccgtaagc gggagaaaac ccagcgtcga atggttgatc tccgtattga tcacgggaaa
      901 aatgcagccg atcgcgtaaa gcaggatgaa ccggctgcgg ttgcgttctt cgctgagcca
      961 ggaagagaac aggatcatga aaacagaaac gtacgccatg cgcacaatgg agcgcttgaa
     1021 gctcacgagc ggatctgcgc tgaacgacga gcaggcgacc atccacgcaa tttccgccag
     1081 cgcaatgagg acgagcgggt gccggataat ttcccggaag cgcgcaggat aaaacaacac
     1141 tgcgcagaac gcggacaccg cgagcaggcc cgtcagcgct tcgccgggca gatcgatcac
     1201 gaaaccattg tccgcgccta ccggcacaga tagcgggacc aggaacagca gcagcccgtt
     1261 cagccatcct ttgcggaaga tgagcatgta cgccacggcg gccagctcgg taaaaagcag
     1321 gagcgaattg cgcagcgaag gaaacgcggg agagagggaa accgcgaaca gcaacgcgaa
     1381 cgcggcaggc aggatgagtt cccggtatcc gttcttctta tgttgctcct gcagctccat
     1441 ctgcatcaat gtttgcgcag cgaggtcacg cgttcacgga acatgaaaaa gaaaacggag
     1501 aaggccagcg aaacgatccc ggcgatcgcc aggttgacgc gcaatgacgg aaatgctttc
     1561 cggtaagaag gcttcgcccg gtcgacgacg aacacgagcg tcaccggccg gttgtattgc
     1621 gtcttcgctt tttcgtaacg gccggaaata tcattgaggc ggaactgttc gaaaatatat
     1681 tcgttcatca cacgctcgag ctgcgtggtg cctgcaggcg tgcccgcttc cgttttcgcc
     1741 tggatcacgt actggctgtt cggcatcacc acaaggttga tcggctgctt tgcccggagg
     1801 tccgccacca gcttttcgag ggaatcgacg acgttttttt ccgcgaggta ttcttttcca
     1861 ccgaacggta agccgccagc tggttgcgct tgtagagatt gttgcgcatg ccgtcggaaa
     1921 tgtcaatgat aaaattcacg atcgcggcgc tgagctccgg ttttttcgtc tgtgcggaaa
     1981 taacgatcga catgtactgc gtgcgttcga acttcactat tttcaggtaa tcgaggtgca
     2041 gcacatcgcg ccagtccggc tccgccgtat ccagctcgaa atagtccgcg aggtggaaat
     2101 gccgctgcac gctgtcgaac atcgcatccg attgcagcaa ttgcatgagg cggtccgctt
     2161 cgagatcata acccacggtg ggattatcca cgatcgattc gacggagtta ttgtttgcag
     2221 gaaaaacaat tccctgcgaa cggtattcct tcggcaggat aaacgtgagc gccgttgcgg
     2281 cgatgagcgc gaccagcacc acgatgccga tgcgctttcc tttgcgggaa aagaattcaa
     2341 ggaattcatt cccttcttcg ttcttcagtt cattttcatt cataaggcaa gtgcctaaag
     2401 tacgacaaag cgcgggggct gacaacgcat cttccttaaa cgttcacggg acatgcgccg
     2461 ctgaatgaat ttgctttcgc gatgcaataa aaaaaaccgc cggaggaatt tcctgcggcg
     2521 gccgaaatga atgcggtaaa aagatcagcg gatgtatact ttgcgtgtaa cggccgtagt
     2581 accgtcgacc acgcggacga aataaacgcc cgtggcctgt ccttccatgc tgatgtgcat
     2641 gtcggcagcg gtgacctctt cttcacggac aacctgcccg gtgctgttgt acacggtcac
     2701 ttttccgtca ggattctgcg caccggtatg aacgaagatg ctgttctcga aggaatgaac
     2761 gcggatattc ccgtcgtttc cgcctgctga aattccaagc ggggaagaag tttgcccggc
     2821 gatgatcgct gaatcagcga tggcttcata ggcataatca cggatgatga tcgtgtccgc
     2881 ccctgcagaa acgtacaggc gtacccagcc gtaatggttc tgcccgccaa tgtcgaaacg
     2941 cacgcccatg aatttatcgc cctgtccgcg gaaatttccg taaagcgcag agccgtaaac
     3001 gctgccgagg tattgggaag gatgcgtggc ataatctacc cagctcgggc tggacggctg
     3061 gatggtatcg ccgctgttga agcgcatcgg gtaagcgtaa tttccctgca cgctgctgat
     3121 gatcgcgttc gtggaaggag aattgaggac gacgctggca taaacgaacg gatagttcgg
     3181 gggtgttgtg ctcacaccga ccttgaattg caattcgggc gtggcgttgt tgtccatatc
     3241 gagatcgtac gtgatggaat catgaacgat cgtgtccggg ttgatgtccg tgtagatgat
     3301 ctgtccgtgg gcggtaatgg caaccgcagt catggatccc gcgagggcgg tgtacgactt
     3361 cagtttgctg ctggtagatt ttttcatgag cgaatgaatt ttttgaacgg gctaaaatag
     3421 gataatattt ttacacggca ggattcccgt cgcacagtgg taatcataca gtgaagttcg
     3481 tatgcatctc tggtcggttt gcataggaac gatagtcttc cgtacgcgtt gccgcccagc
     3541 cttacgcttt ggacccaaaa ctttccaact tttcaactct tcaaacgttg ctcttcctgt
     3601 tcctgtacag ctccccggaa accgcttcag ccatcgccag cgggtccggg ttttctccgg
     3661 tgaatgcggc gatctcctct gcttttgcaa gcgggtcggc aatgacgtcg cgataattta
     3721 cttcgaggaa gctcacgtac ggttgcgctt tcatccaggc ctgcgccttt tcgagctgct
     3781 ttgaaaatgc atcggccagt gtcgtgggat aatttttgtc gtcttttttg ccgagcattt
     3841 tctgctgcga cttcagcacc tcgcctattt cgcgttgcat gaaaatgatg cggtagttgt
     3901 atcccgcagg taaaaactgg agcagcggcg ccaccacttt gatcactttt ccctctgcgt
     3961 ccgccagcca gctgttatcg aggtggagct tcttcacacg ttcatcctcg aaataacctt
     4021 tcgggttgct ttcatcggct tctctttttt catcggaaat aacgtcgagt ccgccggcac
     4081 gcagcatctg catcatcatc gaggtcccgg aacgcggcag cccggaaacg acagtgatcg
     4141 ttccgcggat gaattccttc acgaagcgga tgtgcgcatc cgctttttca ttttcgccga
     4201 gatgctcgct gtaaattttc gcaagccaca tgcgcgcacg ccggttaccg ggcgccatgc
     4261 gcacacacac ctcgaacgcc tgcgcggctt tttcatgatc gccgtatttg tacaaggctt
     4321 cgccgaggtg ataatgcgca gccggcagct ggtatttcag tccgaccgca ttcagcgctt
     4381 catcgatcgc gagctcgatc ttatcctggc gaagcgcggc gaacgcgaga ccgtgatgtg
     4441 cacgcgcatt gtccggatcg atggaaagcg cgcggatgaa cgcacgttcg gcgtccgccc
     4501 agttgcgcgt cgaagtgtag atctgcccga tgagcaggtg aagtccgggc atgtgtgatt
     4561 tgccctgctc cgcacgacgc aaaagcgcga gcgctttcag cgggcgtcct tcggaaaaca
     4621 gcagcgagcc ttcgaggtaa tcgagcggcg gaagatcgtt ttcccgttcc tgctttgcac
     4681 gcagctcgtc aataattttc cgcgcatctt ccgtacgccc ggcctggtga taaacgaacg
     4741 caagacggag cccgaaacga atgacatccg gcacttcgcg gtgcaattct tcgaggaccg
     4801 ggatcgcttc gtcgagccgg ttggtggaga tgaaaacacg cgccaggtaa aaacggcttt
     4861 cgcgcctgat cttgtctgcc gattctgcgt cgtcttctcc caatgcttcc acgtaaccca
     4921 gctcgacaag ctgctgcatc gcctgctgcg ccgcccaggg atcttcacgc acttcgctgg
     4981 aatgctgtcc gctgttcccg cttacgttct cccagctttc gatgaattcc ggcagcacgt
     5041 ttttttctaa aacctgcacc agcgctttcc cgtccatgtc ctttcccacc ggcaaaccga
     5101 aaagtgtcag caccgtcggc gcgatgtcga gcaaagtggc gccatacacg cgttcatcag
     5161 gcgtgatccc gggaccgctg atgcagagca caccgtaagg cgcatgttcc atggcaggag
     5221 cagcaggatc ggtgggcagg tatttcggac ggaggttttc cgaatgaaaa ccgtgatcgg
     5281 acgtaaggat gatgaccgtg tcgtcacccg ccagctccat cagccgttcg agcatcatgt
     5341 cgtggaaacg gtacgcaccg ttgagcacgt ctttgtacaa tgcaaaaagc tcgtcgggca
     5401 tgccggccat ctgcggaggg tggaatttca tgaacgtatg gctgaacagg tcgatctcgt
     5461 tgaggtaaac ggcgagaaaa tcccattcac ggttctgcat gatccacgtg gcgaccgcgt
     5521 ggtacgtcga accggacgcg agcagcgtcg cgatgccgct caggcgcttg tccttgtcct
     5581 ggtcgacctt gtgcgcatcg ggcacgaaag gaagaatgtg cgcgaatgaa atttcacccg
     5641 ggtgcacacg gcattccgca agcgtttccg ccagttctcc cgggtgaacg gaattttccg
     5701 cgacgggcca gggctgatcg aacgcatcat tgggctttgc gaaaaagttg gaaacgtaca
     5761 cgccgtcgag cggttcggca gggtgactcg gccaccagcc gaccgcgtgc gatctcagcc
     5821 cgttctgctg gaggatattc cagagcgctt tcactttccg tgaagtgacg gacgaagggc
     5881 gcacttttcc cgtcaccggg tcgggctcga cgaacccgag aatgccgtgc ttgtcggccg
     5941 ttttccctgt ggaaatggac gtccacagca taggcgacag gggcggatcg agcgtggcga
     6001 tgtttcccat gacgccgcgg gaaatgagct tctgcaatgc aggcatttgc ccgctttcga
     6061 gcagcgggtt gatgatcttc cagtcggccg catcccaccc gatgagcagg acgcgcttcg
     6121 caatgcgttc agccattgtt ctcccttccc gtttttccct ctgatttttc ccgttgctct
     6181 ctccgttttt tccgccatgc tttcagtccg tcaatgccca gggccagcag cccgagcgac
     6241 ccgtcggcgg ggatattata cgtcttcccg gcaggttcat tcacggggtc ttttttcttt
     6301 tccatgtcca aaaataggtg catcttttat tagaaacgct ctaatatatt ttttgaaaaa
     6361 ttgacttatc tttcgggcaa ttcaataaat tattcacaat taagttcttt gcatgaaaac
     6421 tggaacaata aaattcttta atgcaaccaa aggatacggg ttcattaaag aggacggctc
     6481 tggaaacgaa atctttgtgc acgttacggg gctggtcgac caaccggtga acacaggcga
     6541 taaagtatcg tttgaagtat cagaaggccg gaaagggttg aatgccatta acgtcaaaaa
     6601 gatctaaatg catttgcacc tttagaaaaa gctgctccct cccgggcagc tttttttatt
     6661 taccggtttt tcaaaaaaag caggtgacgc atcttaaaca aaacgatgtt aatttttccc
     6721 tgccgcaaaa catccgcttt cctccttcgt tctttctccg ttattttgat ttcccgctac
     6781 gctctatgga ttttatccgc aaacatttcc ggctgatcag cattatcggg ctcgtcctgg
     6841 ttgccgtgat caccgctgca tctgtttatg agctgaaaaa catccgcttc gattacaact
     6901 tcgaaagctt ctttcccgcc ggcgac
//
LOCUS       DASTSF010000293         9660 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1117139,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000293 DASTSF010000000
VERSION     DASTSF010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 9660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9660
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1117139"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..85
                     /locus_tag="VFU15_08520"
     CDS             <1..85
                     /locus_tag="VFU15_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030527 - structural constituent of
                     chromatin [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEU4717865.1"
                     /translation="TGKTIQIKAKKVAKFKAGSDLAGSVNK"
     gene            complement(208..1866)
                     /gene="recN"
                     /locus_tag="VFU15_08525"
     CDS             complement(208..1866)
                     /gene="recN"
                     /locus_tag="VFU15_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016197146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEU4717866.1"
                     /translation="MLSKLTVRNYALIDELSIEFDKGLTIITGETGAGKSILLGALGL
                     LLGDRADSSALMDKTQKCIVEGTFGIKNYSLGNFFSESDLDYSDETTIRREVNADGKS
                     RSFVNDTPVNVTTLRALSEKLVDVHSQHETLTLNDSSFQLSVIDAVAENEELLRKYRD
                     AYSLYRKTLVKLSEVTALEAKSKTDLDYFRFQFDELEKAHLEDGEQEKLETELSTLEN
                     AETIKNGLSSSAFALNGSEENILLSIGTIKQALSQLSKFNPQIAALNERVNSSLIELK
                     DIAAELESLNDDIAVDPKRMEIVQEKLDAIYRLEKKHNVATVAELIALRDSLSEKIAS
                     IGSLEEQIAKLAAETQMQLKKVKELAEKLSGKRSEAAPLLSKSVAGTLKQLGMPNAML
                     DVQLEILPEPRESGTDKATFLFSANKGSAPQPLNKVASGGELSRLMLSLKSLLAERTA
                     LPTIIFDEIDTGISGDVAAKTGSILEKLSRNLQVITITHLPQVASKGKSHLFVYKEES
                     GKRTYTRIRRLNGEERVEEIAKMLSAGKPGDAAMRNAKELLDAK"
     gene            complement(2011..2895)
                     /gene="sucD"
                     /locus_tag="VFU15_08530"
     CDS             complement(2011..2895)
                     /gene="sucD"
                     /locus_tag="VFU15_08530"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HEU4717867.1"
                     /translation="MGVLVGKNSKVIVQGFTGTEGTFHATQMIEFGTNVVGGVTPGKG
                     GTKHLDRPVFNTVSDAVKNTGADVSIIFVPPAFAGDAIIEAAEAGIKVIVCITEGIPV
                     KDMVTAKEYLKGKDCRLVGPNCPGVITAGEAKVGIMPGFVFKKGTIGIVSKSGTLTYE
                     AADQVAKAGLGITTAIGIGGDPIIGTTTKEAVELLMNDPGTEGIVMIGEIGGGLEAEA
                     ARWIKANGNKKPVVGFIAGRTAPKGRTMGHAGAIVGGKDDTAEAKMAILRENGIHVVE
                     SPAQIGKTMAEVLKKVTA"
     gene            complement(3104..4777)
                     /locus_tag="VFU15_08535"
     CDS             complement(3104..4777)
                     /locus_tag="VFU15_08535"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU4717868.1"
                     /translation="MQKRALKNAARALFPVAFTWTIAMMCGCGGGDKVRNDSRQVFRF
                     NELGDVTSLDPAMAGSFENNWALNQLYNGLVEMDSTMNVQPVIAKSWNISDDGMTYTF
                     HLRTDISFQDDPQFPGGKGRKVTAGDFVFSFDRLFDKKVSSASTLVDIIDHDPGKAKD
                     GIEARDDSTLEIHLRQPFKPFLGILTMKYFSVVPKEVVNFYGDGFGTHPVGTGPFMLK
                     FWKDKMNLVLRKNPNYFRFDANGTRLPYLDIVSVTFIKDPEAAFLQFMSGQIDMLSGI
                     DAINKEKVLEKNGELKSEFKDKFTLQQGPFLKTDYLGILVDENIPMVHDNPLRIKYLR
                     QAINYAIDRDKLVRYRRYNLGTPATSGFIPPEMHAYDTKKLRGFSYDPDKARELLDMA
                     GFPGGKGLQPVVLTTTSSYLDIVDEVAHQLNDIGIPAKVEILPPITFKSAVADSKVMV
                     FRKSWICDYPDPENFMSLFYSRHFSPEGTNYTHLHDSEFDGLYERCLTEQNDSIRSDL
                     FLRMDQILVEQSPVIPLYYDRIVRLLQKNVSGLGMDPTNTYNLERVKKI"
     gene            4956..6302
                     /gene="mtaB"
                     /locus_tag="VFU15_08540"
     CDS             4956..6302
                     /gene="mtaB"
                     /locus_tag="VFU15_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008237932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HEU4717869.1"
                     /translation="MNSARTVAFHTLGCKLNFSETSAIGRQLKEAGFAKREFNEGADV
                     FVINTCSVTENADRECRKIVRDALSVSPEAFIVIIGCYAQLKPEEISAIEGVDLVLGA
                     TEKFNLPSYLSEIRKREFADVHSCEIAEADSFVSAWSHGDRTRTFLKVQDGCDYTCSF
                     CTIPLARGKSRSDSIENVIRNAEEIAASGVKEIVLTGINLGDFGKGFHGGKKREENFL
                     ELITALDGINGIERFRISSIEPNLLTDEIITFVAQSEKFVPHFHIPLQSGSDRILKLM
                     RRRYLRDVYTQRAEKIKSLMPHCCIGADVITGFPGETEEDFLETYNFLNALDVSYLHV
                     FTYSERANTDAAEMKDVVPVAERKKRTKMLRILSEKKLRRFYLQNAGTVRPVLFESGN
                     RGEVMYGYSDNYVKVKTDYDPSLANKVCNVRIGEPGHDGIAGVSFSTQPDYSFFNT"
     gene            6311..7534
                     /locus_tag="VFU15_08545"
     CDS             6311..7534
                     /locus_tag="VFU15_08545"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase family
                     protein"
                     /protein_id="HEU4717870.1"
                     /translation="MYPTIYDAVKDLFGLNIPFLKLLQSFGFFVAISFLLCAWVFAKE
                     LHRKEVLGLLKATTRKIIVGARASRQEMAGQFIFGFLVGWKLLSIPFSSGFSANPREY
                     ILSGDGNIFIGVACGTLFSWWRWRQAEKKRLPEPKEEIEKVSAADHVGNMTLLAALFG
                     FLGAKIFHILENIPSFAGPNATQSFSDILISFSGLTMYGGLICGGAAVLIYARRQGFG
                     LFHVMDACAPGLLLAYGVGRVGCQVAGDGDWGIVNTAPKPGWMSFLPNWMWSYDYPNN
                     VNHMCNPDENNPMYQILAQINCDPDHPRLVAPVFPTPFYETVMCLVLFALLWTFRKKF
                     KTPGLLFSVYLICNGIERFLIELIRVDTTMFYLGSFRVVQAEFIAFILFLLGVTGIFL
                     TRKLAKKNAPTIASS"
     gene            7531..8343
                     /locus_tag="VFU15_08550"
     CDS             7531..8343
                     /locus_tag="VFU15_08550"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014680010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEU4717871.1"
                     /translation="MSHLAAGPADLRLLCEEVQELSMRTGKFIREERKKVKRSDIEEK
                     SFNNLVSYVDKTAEKMFVEGLKALLPEAGFISEEDASLPQSETLNWVIDPLDGTTNFL
                     HGVPCFATSVGLVSGNEPVLGVIYEINLDECFYAWKNGGAWMNGERISVSETKQLSHS
                     LVGTGFPYYDHAKLDNYIALFTELTKTTRGIRRPGSAATDIAYVACGRFDAFYEYGLH
                     AWDVAAGAIIVREAGGIVSDFRGGNDFIHGKEFLCGTKDVHAQLLGMIRKYF"
     gene            8423..9190
                     /locus_tag="VFU15_08555"
     CDS             8423..9190
                     /locus_tag="VFU15_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEU4717872.1"
                     /translation="MTKLKLNRPVAFIDLETTGVNVASDRIVEIAILKIFPNGNREMK
                     SMRINPTIPIPKESSDIHHIYDDDVKDEPTFAQAAKQLYAFLEGCDLGGYNSNKFDIP
                     LLAEEFLRAEVEFDVSTRKLIDVQNIFHRMEQRTLAAAYQFYCKKTIENAHSAEADIV
                     ATYEVLEAQLDRYEELKGDVDFLSDFSSRTNNVDLMGRIIYNEKGIPVFNFGKHNGKP
                     VVDVFEKEPSYYNWMMSGDFPLYTKKVITKLRLSMKK"
     gene            9275..>9660
                     /locus_tag="VFU15_08560"
     CDS             9275..>9660
                     /locus_tag="VFU15_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013929313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEU4717873.1"
                     /translation="MKFICIGRNYAEHAKEMNSPVPAEPVIFLKPETALLKGNQPFYY
                     PEFTNDLHHEVEILLKITKMGKHIAPEFAPRYYDEIGIGIDFTARDVQAKCKEKGLPW
                     EKAKGFDGSAVIGKFLPKASFASLSD"
BASE COUNT         2206 a         2587 c         2533 g         2334 t
ORIGIN      
        1 gacgggaaaa accatccaga tcaaagcgaa aaaagttgcg aaattcaaag caggttctga
       61 cctcgcaggt tcagtaaaca agtaatcgcg gcttaccgca taaaaaaacc ctttcacgag
      121 aaagggtttt tttatgtctt aatgtctgcc ctgatcaaaa cgatcgcgaa gaaaaaacaa
      181 cagcaagatg taacgcggcc ggaagaatta tttcgcgtcc agcagttcct tcgcattccg
      241 catcgcagca tcgccgggtt ttcctgcgct cagcattttc gcgatctctt ccacgcgttc
      301 ttccccgttc agcctgcgta tgcgcgtata ggtgcgtttc ccgctttcct ctttgtaaac
      361 gaacaggtga gatttccctt tgctcgccac ctgcggcaaa tgggtgatgg taatgacctg
      421 caggttgcgc gataattttt cgagaatgga gcctgttttg gcggcgacgt cgccggaaat
      481 tccggtgtcg atctcgtcga agatgatcgt cgggagcgca gtgcgttccg cgagcaggga
      541 tttgaggctg agcatgagac gtgaaagctc tccgccggaa gccactttat tcagcggctg
      601 cggcgcgctt cctttattcg cggaaaacag gaacgtcgct ttgtcggttc cgctctcgcg
      661 cggttcgggc aaaatttcca gctgaacgtc gagcattgca ttcggcatgc cgagctgctt
      721 cagtgttccc gccaccgatt tcgaaagcaa tggcgcggct tcgcttcttt tccccgacag
      781 tttctccgcg agctccttca cctttttcaa ttgcatttgc gtttctgcag ccagcttcgc
      841 gatctgctct tccagcgaac cgatggatgc gatcttttca ctgagcgaat cacgcagcgc
      901 gataagttcc gctaccgtgg caacgttgtg ttttttttca agccggtaga tcgcgtccag
      961 cttttcctgc acgatctcca tgcgcttcgg atcgaccgcg atgtcgtcgt taagcgattc
     1021 cagctcggcg gcgatatctt tcagctcgat gagcgaagaa ttcacgcgtt cattcagcgc
     1081 tgcgatctgc gggttgaatt tcgacagctg cgaaagcgct tgttttatcg tcccgatgga
     1141 aagcaggata ttttcttcgc tgccgttcag cgcaaaggcg gatgacgaca aaccgttttt
     1201 gatcgtctcc gcattctcca gcgtgctcag ttcggtttcc agtttttcct gctcaccgtc
     1261 ttcgaggtgc gctttttcca gctcgtcgaa ctggaaacgg aaataatcca ggtccgtttt
     1321 cgatttcgct tcgagcgctg tcacttcact gagcttcacc agcgttttgc ggtaaagcga
     1381 ataggcatcg cggtatttcc ggagcagctc ttcgttttcc gccactgcgt cgatgacgga
     1441 cagctggaaa gaagaatcgt tcagcgtaag cgtttcatgc tgggaatgca cgtcgacgag
     1501 cttttccgag agcgcgcgca aagtggtaac gttcaccggc gtatcattga cgaacgaacg
     1561 tgatttcccg tcggcgttca cttcacggcg tatcgttgtt tcatcggaat aatcgaggtc
     1621 gctttcgctg aagaaatttc cgagcgaata attctttatc ccgaacgttc cttcgacaat
     1681 gcatttctgc gttttatcca tgagcgcgga agaatcagcc cggtcgccga gcaaaagtcc
     1741 caatgcgccg agcaggatgg attttccggc gccggtttcc ccggtgataa tggtgagccc
     1801 tttgtcaaat tcaatgctga gctcgtcaat caaagcgtaa ttgcgtacag tgagtttgct
     1861 taacatatta ccaagttgcc gtcaaaattg ccacaaagtc gtgaaggagc aaagaaaagc
     1921 ggagatgccg ggtgattttt tatgaaccgt gattgtcaaa aaagaaggcc gcccgtggga
     1981 gcagccttcg tcaatatgga taccgtggta ttacgcggtg actttcttca gcacttccgc
     2041 catcgtcttg ccgatctgtg cgggagattc cacgacgtga atgccgttct cgcgcaggat
     2101 cgccatcttg gcttccgcag tgtcgtcttt tccgccgacg atcgctccgg catgtcccat
     2161 cgttcttcct ttgggtgcag ttcttccggc gatgaagccg acaaccggtt ttttatttcc
     2221 gttcgctttg atccagcgcg ccgcttctgc ttcgagtccg ccgccgatct cgccgatcat
     2281 cacgatgcct tcggttccgg gatcgttcat cagcagctcg acagcttctt tcgtcgtcgt
     2341 tccgatgatc ggatcgccgc cgatgccgat cgccgttgta attccgagac cggctttcgc
     2401 cacctggtcg gcggcttcgt atgtgagcgt tcctgatttg gaaacaatac cgatcgtgcc
     2461 ttttttgaaa acgaacccgg gcatgatgcc gaccttcgcc tcgcccgcag tgatgacgcc
     2521 ggggcagttc ggtccgacga gcctgcaatc ttttcctttg aggtattctt tggcggtcac
     2581 catgtctttc acagggatcc cttcggtgat gcagacgatc acttttattc ctgcttccgc
     2641 cgcttcgatg atcgcgtcac cggcgaatgc aggcggaacg aaaatgatgg aaacgtcggc
     2701 gcctgtattt ttcaccgcgt cagaaacggt gttgaatacg ggacggtcga ggtgcttcgt
     2761 gccgcctttg ccgggcgtaa cgccgccaac gacattggtt ccgaactcga tcatctgcgt
     2821 tgcgtgaaac gtgccttcgg tcccggtgaa accctggaca atgacttttg aattttttcc
     2881 gacgagaact cccatataaa gattgaatga ttgaaagact ggctgatttt aataagggca
     2941 aaaataacgt tttgaacgga ttgccgtgtt cccgcaaagg gcagttttca agatcatttc
     3001 aaccgttccg gggcggaatg tttttcccat cttcatgcaa gtcatcgctc cggtccagga
     3061 caaatgcagg ccttatggac cggtcaacgc ctacaacccg ggattatatc ttcttcaccc
     3121 gctccaggtt gtaggtgttg gtcggatcca ttccaagtcc ggaaacattc ttctgcaaaa
     3181 gacggacgat gcgatcgtaa tacagcggga taacaggcga ctgctcgacg aggatctggt
     3241 ccatgcgcag gaaaagatcg gaacggatgg aatcgttctg ctcggtgagg cagcgctcgt
     3301 acaggccgtc gaattcggaa tcgtgcaggt gcgtgtagtt cgttccttcc gggctgaaat
     3361 gccggctgta gaagagcgac atgaaattct ccggatcggg ataatcgcag atccacgatt
     3421 tgcggaacac catcactttc gaatccgcta ctgctgactt gaacgtgatg ggcggaagta
     3481 tttccacttt cgcaggaatg ccgatgtcgt tgagctggtg cgccacctcg tcgacgatat
     3541 cgagatacga tgaggtggtc gtcagcacga ccggctgcaa tccttttcca ccgggaaagc
     3601 cggccatgtc gaggagctcg cgcgccttgt cgggatcata ggaaaacccg cgcagcttct
     3661 tcgtatcgta cgcgtgcatc tcgggcggaa tgaacccgga agtagcgggc gtgccgaggt
     3721 tgtaacgccg gtaacgcacg agcttgtcgc ggtcaatggc gtagtttatt gcctgtctta
     3781 aatatttgat gcgtaatgga ttatcgtgaa ccatcggaat gttttcatca accagtatcc
     3841 cgaggtaatc tgttttgaga aaaggtccct gctgcagcgt gaatttgtcc ttgaattcgc
     3901 ttttcagctc cccgtttttc tccagcactt tttccttgtt gatggcgtct atgcccgaaa
     3961 gcatatcgat ctgtccgctc atgaactgca ggaaagccgc ttcgggatct ttgatgaagg
     4021 taaccgaaac gatgtcgaga taaggaaggc gcgtgccgtt tgcatcgaaa cggaaatagt
     4081 tcggattttt ccggagcacg aggttcatct tgtctttcca gaatttcagc atgaacgggc
     4141 cggtaccgac aggatgcgtt ccgaaaccgt cgccgtaaaa attcacgact tctttgggga
     4201 cgacggaaaa atatttcatc gtcagaatgc cgagaaaagg cttgaacggc tggcgaagat
     4261 gaatctcgag cgtggaatca tccctggctt caataccgtc tttggctttg cccggatcgt
     4321 gatcgatgat atcgacgagt gtcgatgcgc tggaaacttt cttgtcgaaa aggcggtcga
     4381 agctgaacac gaaatccccg gcggtcactt tcctgccttt tccgccggga aactgcggat
     4441 cgtcctggaa gcttatgtcc gtgcggagat ggaacgtata cgtcatcccg tcgtcgctga
     4501 tgttccagct tttcgcgatc acgggctgca cgttcatcgt cgaatccatt tccacgagac
     4561 cgttgtacag ctggttcagg gcccagttgt tctcgaaaga gccggccatc gccggatcga
     4621 gagaagtgac atctcccagc tcgttaaagc gaaagacctg gcgcgaatcg ttgcgcactt
     4681 tatcaccgcc gccacaaccg cacatcatcg cgatcgtcca cgtaaaagca accgggaaaa
     4741 gcgcccgtgc cgcatttttc aaagcccttt tttgcatgta acaagtttag cactttgcag
     4801 cccgcaggaa gttcaggcgt gacgttacat tttaaacagg tttttcaaca ttggccgcaa
     4861 tcgaaaaaga aaatcttccc gcagaatccg gcaggtcgat tttcatttcc ccgaacggtg
     4921 agaaagggac gcagttttag tacctttacc gcccaatgaa ttccgccagg acagttgctt
     4981 ttcacacgct cggctgcaag ctcaattttt cggaaacgtc cgctatcggc aggcaactga
     5041 aagaagccgg ttttgcaaag cgcgaattca atgagggcgc agatgttttc gtgatcaaca
     5101 cctgttcggt caccgaaaac gccgacaggg aatgccgcaa gatcgtccgc gatgcgcttt
     5161 ccgtttcacc cgaagcattt attgtcatca tcggctgcta tgcgcagctg aagccggagg
     5221 aaatttcagc gatcgaaggc gtggacctcg tgctcggcgc gacggagaaa ttcaaccttc
     5281 cctcctacct gtccgaaata cgcaaacgcg aattcgccga tgtccattcc tgcgaaatag
     5341 cggaagccga ttcgttcgtc agcgcatggt cgcatggcga ccgcacgcgc acgttcctga
     5401 aagtgcagga cggatgcgat tacacctgtt cgttctgcac gatcccgctc gcacgtggaa
     5461 aaagcaggag cgacagcatt gaaaatgtca tacgcaatgc ggaagagatc gcagcatcag
     5521 gagtgaaaga gatcgttctt accgggatca acctcggtga tttcgggaaa gggtttcacg
     5581 gcgggaaaaa gcgcgaagag aatttcctcg agctcatcac tgcgcttgac ggcataaacg
     5641 gcatcgaacg tttccgcatt tccagcatcg aacccaacct gctcacggac gagatcatca
     5701 ccttcgtggc gcagtcggaa aaattcgttc cgcatttcca tattccgttg cagtccggtt
     5761 ccgaccggat cctgaagctc atgcgccggc gctacctgcg tgatgtttac acgcagcgcg
     5821 ctgaaaaaat aaaatcgctc atgccgcatt gctgcatcgg cgccgacgtc atcaccggtt
     5881 ttcccggcga aacggaagag gatttcctcg aaacctataa tttcctgaac gcgctggatg
     5941 tttcctacct gcacgttttt acgtattcag aacgtgcgaa caccgacgcg gcggaaatga
     6001 aagacgttgt tcccgtcgca gaacggaaaa aacgaacgaa aatgcttcgc atcctttccg
     6061 aaaaaaagct gcgccgtttt tacctgcaga acgcagggac cgtgcggccc gtgcttttcg
     6121 agagcgggaa ccgcggcgaa gtgatgtacg gttattccga taattatgtc aaagtaaaaa
     6181 cggactacga tccttcgctg gcgaataaag tgtgcaacgt ccgtatcggg gagcccggcc
     6241 acgacggaat cgccggcgtt tcattcagca cgcaaccgga ttattcattt ttcaacactt
     6301 aaactcccgt atgtatccca ccatttacga tgcagtaaaa gatcttttcg ggctgaacat
     6361 cccgttcctg aaactgctgc agagcttcgg atttttcgtc gcgatctctt ttcttctctg
     6421 cgcatgggtc ttcgccaaag agctgcaccg caaggaagtg ctcggcctgc tgaaagcgac
     6481 cacgcggaag atcattgtcg gtgcgcgcgc ttcccgccag gaaatggccg ggcagttcat
     6541 cttcggattt ctcgtcggct ggaagctgct ttccattcct ttttcgtccg gcttcagcgc
     6601 gaatccgcgt gaatacattc tctccggcga cggaaatatt ttcatcggcg ttgcctgcgg
     6661 aacattgttt tcctggtggc gctggagaca ggcagaaaag aaacgtcttc cggaaccgaa
     6721 agaagaaatt gaaaaagtga gcgcggcaga tcacgtgggg aacatgacgc tgctcgctgc
     6781 gctgttcggc tttctcggtg cgaagatctt ccacatcctc gagaacattc cgagtttcgc
     6841 cggtccgaac gcgacgcagt ctttctccga cattctcatt tccttcagcg ggctcacgat
     6901 gtacggcgga ctcatctgcg gcggcgcggc cgttctcatt tacgcacgca ggcagggctt
     6961 cggacttttt cacgtgatgg acgcatgcgc gcccggactt cttctcgcct atggcgtggg
     7021 acgtgtcggt tgccaggtgg ccggcgacgg cgactgggga atcgtgaaca ccgcgccgaa
     7081 accggggtgg atgagctttc ttccgaactg gatgtggtcg tacgattacc cgaataacgt
     7141 caaccacatg tgcaacccgg acgagaacaa cccgatgtac cagatcctcg cacagatcaa
     7201 ttgcgacccg gaccatcccc gtctcgtggc accggtcttc cccaccccgt tctacgaaac
     7261 cgtcatgtgc cttgtacttt ttgcgttgct ctggacgttc cggaaaaaat tcaagacgcc
     7321 cgggttgctg ttcagcgttt atctgatctg taacggcatc gagcgtttcc tcatcgaact
     7381 gatccgcgtc gacacgacga tgttctatct cggttcgttc cgtgtcgtgc aggcggaatt
     7441 catcgcgttc attctcttct tgctcggtgt gacgggaatt ttccttaccc ggaagctcgc
     7501 gaagaagaac gcaccaacca tcgcttcttc atgagccacc tggctgcggg accggctgat
     7561 ctcaggctgc tctgcgagga agtgcaggaa ctgagcatgc ggaccgggaa attcatccgc
     7621 gaggagcgga agaaagtgaa gcgttcggac atcgaagaaa aaagcttcaa caatcttgtt
     7681 tcctacgtcg acaaaacggc ggagaaaatg ttcgtggaag gactgaaagc gcttcttccg
     7741 gaagcgggtt tcatttcaga agaagatgct tcgctgccgc aaagcgaaac gctgaactgg
     7801 gtgatcgacc cgctcgacgg cactaccaat ttcctgcatg gtgttccctg cttcgccact
     7861 tcggtcggac ttgtttcagg taacgaaccg gttctcggcg tgatctacga gatcaacctc
     7921 gacgaatgtt tttacgcgtg gaaaaacggc ggcgcgtgga tgaacgggga acgcatttct
     7981 gtttccgaaa cgaagcagct ttcccattcg ctcgtgggaa ccggctttcc gtattacgat
     8041 cacgcgaagc tcgacaacta tatcgcgctg ttcaccgagc tgacgaaaac cacgaggggg
     8101 atacgccgtc cgggttcggc ggcaacagat attgcatacg tggcctgcgg tcgcttcgat
     8161 gcattttacg aatacggttt gcacgcgtgg gatgtggcgg caggtgcgat catcgtacgc
     8221 gaagccggcg ggatcgtttc ggatttcagg ggcggtaacg atttcattca cgggaaggaa
     8281 ttcctgtgcg ggacgaaaga cgttcacgcg cagctgctcg gaatgatccg gaagtatttc
     8341 tgagtctcat catgaccatt gctgaaaaat ccttcgtgaa acaatccctg cgtataaaca
     8401 acagcctgaa ttttcaatca aaatgaccaa actgaaactc aaccgccccg tcgctttcat
     8461 cgatctcgag accaccggcg tcaatgtcgc ttccgaccgc atcgtggaaa ttgccatcct
     8521 gaaaattttc ccgaacggga acagggagat gaaaagcatg cgcatcaacc cgacgatccc
     8581 cattccgaaa gaatcatccg acatccatca catttacgat gacgatgtga aagacgagcc
     8641 cacgttcgca caggcggcga agcagctgta cgcttttctc gaaggctgcg atctcggcgg
     8701 ctacaattcc aacaaattcg atatcccgct gctggctgaa gagttcctcc gtgcagaagt
     8761 ggagttcgac gtttccaccc gcaagctgat cgacgtgcag aatattttcc accgcatgga
     8821 acagcgaacg ctcgctgcgg cttaccagtt ctactgcaag aaaacgatcg agaatgcaca
     8881 ttcggcggaa gcggacattg tcgccacgta cgaagtgctg gaagcgcagc tcgatcgtta
     8941 cgaagagctc aaaggcgacg tggatttcct ttccgatttt tcatcgcgca cgaacaacgt
     9001 cgatctcatg ggacgcatca tttacaatga gaaaggcatt ccggtcttca atttcggaaa
     9061 acataacggc aagccggtag tggatgtttt tgaaaaagag ccgagctatt acaactggat
     9121 gatgtcgggc gatttcccgc tgtacacgaa gaaagtcatc acgaaactga ggttatcgat
     9181 gaagaagtaa ttggttatcg caaacgggat ctgcacccgg cagacataaa acgtttaaac
     9241 acccgacacg gttcaccact caatacgcac tgccatgaaa ttcatctgca tcggccgcaa
     9301 ctacgcggag cacgcgaaag aaatgaacag ccccgttccg gcggagcccg tgatctttct
     9361 caagccggaa accgcgctgc tgaagggcaa ccagccgttc tattatcccg aattcacgaa
     9421 cgatcttcac cacgaagtgg aaattctgct gaagatcacg aagatgggca aacacattgc
     9481 cccggaattt gccccgcgtt attatgacga gatcggtatc ggcatcgact tcacggcgcg
     9541 tgatgtgcag gcgaaatgca aggagaaagg attgccctgg gaaaaagcga aaggcttcga
     9601 cggttctgcc gttatcggga aattcctgcc gaaggcttcg tttgcctcgc tcagtgacat
//
LOCUS       DASTSF010000294         2302 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1889509,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000294 DASTSF010000000
VERSION     DASTSF010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2302
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1889509"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(184..1251)
                     /gene="pdxA"
                     /locus_tag="VFU15_08565"
     CDS             complement(184..1251)
                     /gene="pdxA"
                     /locus_tag="VFU15_08565"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HEU4717874.1"
                     /translation="MSEANKIVVGITQGDFNGIGPEVIIRTLSDPMLCELCTPVVFSS
                     QKVMNFWRKTTGLEEFNFQVVRDLDQLNPKRANLFSFFEEDVEVEPGKPLPLAGKYAI
                     RSVDVACEALLNGKIDVLVTAPLDKNSVAAEIADFNGHTEYLRKKLGAESSIMLLVNE
                     DLRVGLVTDHVPLREVAAYINKDRIVSRAKLMAKSLLEDFGVRKPKIAVLGLNPHAGD
                     GGTLGKEELEIIAPAVAQLRSENVLAFGPYSADGFFGSGNYRNFDGILAMYHDQGLGP
                     FKTLSFSGGVNFTGGMKYVRTSPDHGTGYDIAGRNEANESSFRQAVFTAIDVFRKRNN
                     WEEISADPLKPQFRQKESRER"
     gene            complement(1304..>2302)
                     /locus_tag="VFU15_08570"
     CDS             complement(1304..>2302)
                     /locus_tag="VFU15_08570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717875.1"
                     /translation="LVKYSFANGEAGPQEKIMDVVMQNPDDKTPRIRFDLGPNQVYRN
                     RYVITAYGNVVDLDQKKILVDQHDRFVRASGDSLIFYTNDIFKGKYYSVLNLVTGKYE
                     QVKDPLFRAITGKDVEPDCSQRIFRIYYYPPSAPKIELVRDAGYGEDISLIPGARPRL
                     PLFWIDDDNFLYPNYSSAHDYVAIMKVSVSAKSEEKIGDVDQLPENRALSEFFRDADG
                     QIEYRCSRGLYRVDVEKKKVTEMQFIPEGNGFSISSNDVDSKGRNIRSGETVIGNYYC
                     DPSLASTGTGVIAFPYEMVLGGDHYLQGAAVWCASSSKWKTVGDSDLSAVVGWIEE"
BASE COUNT          558 a          612 c          576 g          556 t
ORIGIN      
        1 ctgaacggga tcacaaattc cttccttttt tccatccggc aacctttttt ggaaaggtgg
       61 caaaactaca taaattaaca ggatatcaac aaaaaaacaa tcgtcaaaaa cctgtttttc
      121 gctgaaaacg ggctaaaaac cgatgaaaaa aggggaaatg tttcgaaaaa gttaaaatca
      181 gccctatctc tcccgtgatt ccttttgccg gaactggggt ttcagcggat ctgcggagat
      241 ctcttcccag ttgtttcttt tccggaaaac atcaatggcc gtaaaaaccg cctggcggaa
      301 ggaggattcg ttcgcttcgt tcctgccggc gatatcgtag cccgtgccgt gatcaggtga
      361 tgtgcggaca tatttcattc caccggtaaa attgacacca ccggaaaatg aaagtgtttt
      421 gaaaggcccg aggccctggt cgtggtacat ggccaggatg ccgtcgaaat tgcggtaatt
      481 gcctgagccg aaaaacccgt cggcggaata aggtccgaaa gcaagcacat tttcactccg
      541 cagctgtgca accgccggag caatgatctc cagttcttct ttccccagcg taccgccgtc
      601 accggcatgc ggattgaggc cgagcaccgc gattttcggt ttccgtacgc cgaaatcctc
      661 cagcagcgat ttcgccatga gctttgcgcg ggaaacaatg cggtctttat ttatatatgc
      721 tgccacttcg cggagcggga cgtgatccgt cacgagcccg acacgaagat cttcgttgac
      781 aagaagcata atggacgatt ccgcaccgag ctttttgcgc aggtattccg tatggccgtt
      841 gaaatcagct atttccgcag caacagaatt cttatccagc ggggcagtga ccagcacatc
      901 gatcttcccg ttcagcaaag cttcgcaggc cacatcgacc gatcggatgg catattttcc
      961 tgccagcggc aaaggtttcc cgggttccac ctcgacatcc tcctcaaaga agctgaacag
     1021 gtttgccctt ttcgggttaa gctgatcgag atcgcggaca acctggaaat tgaattcctc
     1081 aagaccggtt gttttgcgcc agaaattcat cactttctgg ctggaaaaaa ccacgggcgt
     1141 gcaaagctcg cacagcattg gatcggacag cgtgcggatg atcacttcag gtccgattcc
     1201 gttgaaatca ccttgcgtga taccaacaac gatcttattc gcttcgctca ttccgttaca
     1261 atcagtttcg ttgaatcagg gcaaatgtac gcaacacgca ggatcattct tcgatccacc
     1321 cgacgacggc ggaaagatca ctgtcgccga cggttttcca tttggaagaa gatgcgcacc
     1381 agaccgcagc gccctgcagg taatgatcgc cgcccagcac catctcgtaa ggaaaagcga
     1441 tgacgcccgt ccccgtggat gcgagcgagg gatcacagta atagttcccg atcactgttt
     1501 ccccgctgcg gatattcctt cctttgctgt cgacatcatt cgaggaaatg gaaaagccgt
     1561 tcccttccgg gatgaactgc atttccgtca cttttttctt ttcgacatcg acacggtaaa
     1621 gtccgcggct gcaccggtat tcgatctgcc cgtccgcatc gcggaaaaac tcggagagcg
     1681 ccctgttctc ggggagctga tcgacatcgc cgatcttctc ttccgatttt gcgctgacgg
     1741 aaactttcat gatcgccacg taatcgtgcg cggaggaata gttcgggtaa aggaaattgt
     1801 cgtcgtctat ccagaacagc ggaaggcgcg gacgtgctcc cggaatgagg gaaatatctt
     1861 ctccgtaacc cgcatcgcgc accagctcga tcttcggcgc ggaaggcgga tagtaataga
     1921 tcctgaaaat acgctgggaa caatcaggct ccacgtcttt ccctgtgatc gcacggaaca
     1981 gcgggtcttt cacctgttcg tatttcccgg taaccaggtt gagcacggaa taatatttcc
     2041 ctttgaaaat atcgttggtg taaaaaataa ggctgtcgcc ggatgcgcgc acaaaacggt
     2101 cgtgctgatc gacgaggatc tttttctgat cgaggtcgac gacatttccg taagcggtga
     2161 tgacgtaacg gttgcgatac acctggttgg gaccaagatc aaagcggatg cgcggtgttt
     2221 tgtcgtccgg gttctgcatg accacatcca tgatcttttc ctgcggacct gcttctccgt
     2281 tcgcaaaaga atatttgacg ag
//
LOCUS       DASTSF010000295         8130 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1131194,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000295 DASTSF010000000
VERSION     DASTSF010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 8130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8130
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1131194"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1765
                     /locus_tag="VFU15_08575"
     CDS             <1..1765
                     /locus_tag="VFU15_08575"
                     /inference="COORDINATES: protein motif:HMM:NF036482.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 2 protein"
                     /protein_id="HEU4717876.1"
                     /translation="LPPGGTLSDVRFVFPVVHPRLWWCTGYGKQELYDFTLTLKCGKD
                     ISAQTTRTGLRTIELTTNTVEYDGQMAFILNGKKIYMRGANWIPCDNFLPRVTTEKYY
                     SLLKNAKDCGMNMLRVWGGGVYEDDRFYNICDSLGIMVWQDFMFAGGVYPASVHFMDI
                     AGKECMDQTERLAGHPCVSLFCGNNEIAEGWMNWGWQKEMNYSKADSLALVAEMLQLD
                     NGIRLVMKTNAANIPYWPSSPSTGWGHKEAFEQGDVHYWGVWWGMEPFSAYDDHIGRF
                     MSEFGFQGMPDMRTFFDFTGRKALSLNDADVISHEKHPKGFETINTYMARDYPVPSSF
                     DDYVYVSQVMQADGVTRAIEAQRHTRPYCMGSLFWQYNDCWPVTSWSSVDYYGRPKLL
                     QYRLKEAYAPVMMDVLQKDDTAYVYIVNDDTLLHTGVLGFRWMNMAGNNERYGINPFR
                     VPAEHSRLALILPFKLLEDSVDRTKGFLELVFNYENGKVLKKTVCLTSDRNMQFVKDP
                     GISFDVSQDPDRKNEFTVSLRAVSYAKDVFLSLDDPSATFSDNGFDLIPSEETDVKIT
                     TSLNEDELKRSLVIKSMNQVK"
     gene            complement(1809..2639)
                     /locus_tag="VFU15_08580"
     CDS             complement(1809..2639)
                     /locus_tag="VFU15_08580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717877.1"
                     /translation="MKKFLLLLIVVLATGLGLCAQDTIVKVNGDRVVAKVTEVGQYTI
                     RYKRYNAPDGPVYDVKKTDVRMIVYSDGTIEHYSAADNGYSNPSSENNIRRRWLIGLN
                     SFDMLFGVLTASVQYNIVGDELALRVPLSAGVSHLAGNSPTSSPGISYYYYSRDKIFS
                     SGLDLLYYPAKQHRVASYYTGVSLEYGEIYTYDRYYYVYPYTPHYSTYWFSGTGIING
                     FLFQPSDRVNISLDATIGMAISNGETNYYSSSNIGPLIRMGMTLGYRFGNNILADKTK
                     "
     gene            complement(2704..5109)
                     /locus_tag="VFU15_08585"
     CDS             complement(2704..5109)
                     /locus_tag="VFU15_08585"
                     /inference="COORDINATES: protein motif:HMM:NF039587.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HEU4717878.1"
                     /translation="MRKLLLPVFFFFVTTAAAQDPVRVMEAVRAAIAPAIDGDLDDSC
                     WSKAPHTERFIQNAPVPGNPSSFDEDVRVVYDDNAIYIGARLYDPSPDSILHQLSARD
                     DYQGNSDAFGVMLDTYHDRRNAFFFAVTAAGVQADSRYTFDTEDLSLNSVWYSKTKVD
                     AKGWTVEMKIPYQAIRFRNGDDQLWGLNFTRIIRRYREQSWWNFVDPNVTGVVNQCGD
                     LAGINNIVAPVRLALLPYVSAYAENYAGRNAYSLNGGMDIKYGINESFTLDMTLIPDF
                     GQTQSDNVVLNLSPFEVKFDERRYFFTEGTDLFNKNDLFYSRRIGAAPPGYSTVSNQL
                     DTSEIVYDNPEVTRLYNAVKISGRTKNKTGIGFLNAVSAPAYATLRDTVTGDLRRVET
                     AVLTNYNVLVIDQVLPHNSYIGFLNTSVMRNGNARDADVSSVQFRAGDRRNVFAVDGY
                     TDVSNVWNAGSAKPSTGYRYNFTAGKVSGKYTAIARYKVISQNFDSNDLGYLDLRNNM
                     DFGLQQNYNIHTPVGRILWQLHRLDLDLGVFYNPRHYTFFTITGRHIFTFRNWMSVGV
                     NWLANPIYSYDYYETRTPGRYLLYPKNYQLSGFLSSDYRKKLALDLTAAYRIFLQRNR
                     TIASYSVAPRWRVNNKLLIVYSWAQELKNDNVGFVANRNDSLFFGLRNLNTVTNTLTL
                     DYIFTNTMGLSLRVRHYWSQADYKGYYLLNANGQTQDAYYNGDADVRFNAFNVDMVFT
                     WQFFPGSELSVVWKNAILTTDNLLKANYAEDARDVFDSPQSNSLSAKLIWYIDAGKWF
                     RRK"
     gene            5642..>8130
                     /locus_tag="VFU15_08590"
     CDS             5642..>8130
                     /locus_tag="VFU15_08590"
                     /inference="COORDINATES: protein motif:HMM:NF024962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprB repeat-containing protein"
                     /protein_id="HEU4717879.1"
                     /translation="MKKILLASVLIFSGFFSGRLHAQMTTFRFSYDVGLFDISGGMVQ
                     NPAGDFVIAGLNNSFGPYYGNAIKVDAQGNVVWAKAYTGGFATSFNDIKNVSTGGYIM
                     TGQSTSGGGGALLVRIDANGNVLWAYRYQLPDIPSGNASNEYGNAVIETSDGGFLVGG
                     GVDYFWDGVSANTVDTTSAMGFKVNSSGTLQWSKVWTIATANPDEHYINDVAESADGY
                     FFVGQSSEGSGTLSSNGDYPSNALIIKTTTAGALTYIRRWGAGNTSSQGINCAITLST
                     GNILLGGFDDVNAFIVSISGTGSSTPTVIFNKRLNGSIFGNTFLLEDIMENSDGNYSM
                     IGTQLAFLSVSLNTMIGKINSSTGAWMFGRTYAPIGLSAILPEGGLCSDQGYFVSMTD
                     QQAAGFNYNVIRTNTSGQTNDPASGCPGTSITPTLGSESVTFSTPTSSNYDLMTSSTW
                     TPVVQNLTPTRTQHCYNAPTPLSTTSTSTNVTCFGGCNGTATANPSGGTAPYTYSWNS
                     TPVQTTQTATGLCPGSYTCTVTDNASATTTVTVNITQPVSALSASIPSPTNVTCNGSC
                     NGSATVNASGGTSGYTYSWNSAPVQTTATASNLCAGSYTVTVTDANSCTTTQSVTITQ
                     PSAITASATSTPSSCSSNTGTATVSAGGGAGGYTYSWAPSGGTGATANNLAPGNYTVT
                     VHDANNCTQTATVNVTTTTGPSASLQSQSNVSCNGGTNGSATVNASGGSPGYSYSWTS
                     GGSSATENNLPAGNYTCTITDAAGCVTTQSVAITEPSVLSPSSTTSPTGCTSSGSATE
                     TPSGGTSPYTYAWSSGGTAATESNLGVGTYT"
BASE COUNT         1919 a         2291 c         2155 g         1765 t
ORIGIN      
        1 ccttcctccc ggcggaacat tgtccgatgt gcgcttcgtg ttcccggtcg tccacccgcg
       61 cctgtggtgg tgcaccggtt acggaaaaca ggagctctac gattttacgc tgacgctgaa
      121 atgcggaaaa gatatttccg cgcagacgac acgcacgggc ctgcgcacga tcgaactcac
      181 gaccaacacc gtcgaatacg acgggcagat ggccttcatc ctgaacggaa aaaaaatata
      241 catgcgcggc gccaactgga taccgtgcga taattttctt ccacgcgtaa cgactgaaaa
      301 atattattcc ctgctgaaga acgcgaaaga ctgcggcatg aacatgctgc gcgtttgggg
      361 cggcggcgtt tatgaagacg atcgttttta caacatctgc gattcgctcg gcatcatggt
      421 gtggcaggat ttcatgttcg ccggcggcgt ttatcctgct tcggttcatt tcatggacat
      481 tgccgggaaa gaatgcatgg accagaccga gcgtcttgca ggtcatccct gcgtttcact
      541 tttctgcggc aacaacgaga tcgcggaagg atggatgaac tggggctggc agaaagaaat
      601 gaattattcg aaagcggatt cgcttgcgct ggtggcggaa atgctgcagc tcgataacgg
      661 catccgcctc gtcatgaaaa cgaacgcagc gaatattccg tactggccat cttccccgtc
      721 gacaggatgg ggacataaag aagcgtttga gcagggcgac gtgcattact ggggcgtgtg
      781 gtggggcatg gaaccgttca gcgcgtatga cgatcacatc ggccggttca tgagcgaatt
      841 cggtttccag ggaatgcccg acatgcgcac gttcttcgat ttcaccggca ggaaagcgct
      901 ttcgctgaat gacgccgacg tgataagcca cgaaaagcat ccgaaaggtt ttgaaacgat
      961 caacacctac atggcacgcg attatcccgt accgtcttcg ttcgacgatt acgtgtacgt
     1021 ttcgcaggtg atgcaggccg acggcgtgac acgggcgatc gaggcgcagc ggcatacgcg
     1081 cccctattgc atgggttcgc ttttctggca atacaacgac tgctggcccg tcacttcctg
     1141 gtcttcggtc gattattacg gacgaccgaa actcctgcag taccggttga aagaagctta
     1201 cgcgccggtg atgatggacg tgctgcagaa agacgacacc gcgtacgttt acatcgtcaa
     1261 cgacgacacg ctgctgcata ccggcgtgct cggtttccgc tggatgaaca tggcgggcaa
     1321 caacgaacgt tacggcatca atcctttccg cgttcctgcg gaacattcac gtctcgcgct
     1381 gatccttccg ttcaaattac tcgaggacag cgtcgatcgc acgaaaggat ttctcgagct
     1441 cgtgttcaat tatgaaaacg ggaaagtgct gaagaaaacg gtttgcctca cgagcgacag
     1501 gaacatgcag ttcgtgaaag atcccgggat atcgttcgac gtttcgcagg atcccgacag
     1561 gaaaaacgaa ttcaccgttt cgctgcgcgc cgtatcgtat gcaaaggatg tttttctttc
     1621 gctcgacgat ccgtccgcca cgttcagcga taacggtttc gacctcattc cttccgaaga
     1681 aaccgatgtg aagatcacga catcgctcaa tgaagacgag ctgaagcggt cgctggtgat
     1741 aaaatcgatg aaccaggtaa aatgaacgcg cttcggcatg atttgccgga acgcagcgcc
     1801 ttccgtttct atttcgtttt atccgcgagg atattattcc cgaaacgata gcccagcgtc
     1861 attcccatcc ggatgagagg accgatgttg ctgctgctgt aataattcgt ttccccgttc
     1921 gagatcgcca ttccgatcgt tgcatccagc gagatattca cccggtcgga aggctggaaa
     1981 agaaacccgt taatgattcc tgttccggaa aaccagtacg tgctgtaatg cggcgtataa
     2041 ggataaacgt agtagtaacg atcatacgta taaatttctc cgtattccag cgacacacct
     2101 gtatagtagc tcgccaccct gtgctgcttc gcaggataat aaagaagatc gaggccggag
     2161 ctgaatatct tgtcgcggct gtaataataa tacgagatgc ccggcgaact ggtcgggctg
     2221 ttgcctgcca gatgtgaaac gcctgcactg agcggaacac gcagcgccag ctcatcgccg
     2281 acaatattgt attgcacgct ggcggtcagc accccgaaca gcatatcgaa agaattgaga
     2341 ccaatcagcc agcgtctcct gatgttattt tcagaagaag gatttgaata gccgttatca
     2401 gcagcagaat aatgttcgat cgttccgtca gaatagacga tcattctcac atctgttttt
     2461 ttgacatcgt aaacgggacc gtcgggcgca ttgtagcgct tgtacctgat cgtatattgt
     2521 cccacctccg tcactttcgc aacgacgcgg tcgccgttca cctttacgat cgtgtcctga
     2581 gcacacagtc cgagacctgt ggccaatacg acaataagaa gcagcaggaa ttttttcatg
     2641 gtcaggagtt ttttgaaaag acgtgcggaa tggggttgag ttgcttaacc ctaaagataa
     2701 aaatcatttc cgtcggaacc acttcccggc atcgatgtac cagatcagct ttgcggaaag
     2761 gctgttgctc tggggcgaat cgaaaacgtc gcgcgcatct tcggcgtaat ttgccttcag
     2821 caggttatcc gtggtgagga tcgcattctt ccagacaacg ctcagctcgc tgcccggaaa
     2881 gaactgccac gtgaacacca tgtcgacatt gaacgcgttg aaacgtacgt ccgcgtcgcc
     2941 attgtaatac gcatcctgtg tctgtccatt cgcgttaagc aggtaatatc ctttatagtc
     3001 cgcctgcgac cagtaatgac ggacgcgcag cgaaagcccc atcgtattgg tgaaaatgta
     3061 gtccagcgtc aacgtgttcg tcaccgtgtt caggttgcgc aaaccgaaaa agagcgaatc
     3121 gttgcggttg gccacaaatc ccacattgtc gtttttcaat tcctgcgccc agctgtagac
     3181 gataagcagc ttgttgttca ccctccaccg cggcgcgacg ctgtacgagg cgatggtgcg
     3241 gttgcgctgg aggaaaatac ggtatgccgc ggtaagatcg agcgcgagct ttttccggta
     3301 atcgctggat agaaacccgc tgagctggta attcttcggg taaagcaggt aacgtccggg
     3361 cgtgcgcgtt tcataatagt cgtagctgta aatgggattc gccagccagt tcacaccgac
     3421 ggacatccag ttgcggaacg taaaaatgtg acgccccgtt atcgtgaaaa acgtataatg
     3481 ccgcgggttg taaaaaacgc cgaggtcaag atcgagacgg tgcagctgcc agagtattct
     3541 tcccaccggc gtatggatgt tgtaattctg ctgtagaccg aaatccatgt tgttgcgcag
     3601 gtcgagatag cccaggtcgt tcgaatcgaa attctgcgag atcactttgt accgtgcgat
     3661 cgccgtgtat ttaccgctca ctttccccgc ggtgaaattg tagcggtaac cggtcgacgg
     3721 tttcgcgctt cctgcattcc acacgttgct gacatccgtg taaccgtcca ctgcgaacac
     3781 attccgccgg tcgcccgcgc ggaactgcac gctgctcacg tctgcatcac gcgcgttccc
     3841 gttgcgcatc acgctcgtgt tcaggaaacc gatgtacgaa ttatgcggaa gcacctggtc
     3901 gatcaccagc acattgtagt tcgtcagcac cgctgtttcc acgcgccgca ggtcgccggt
     3961 gaccgtatcg cgcagggtgg cgtacgcagg cgcgctgacc gcattgagaa aaccgattcc
     4021 cgtcttgttt ttcgtacggc cggaaatttt caccgcgttg tacaggcgcg tcacttccgg
     4081 gttgtcgtaa acaatttcac tcgtgtcgag ctggttcgaa accgtgctgt aaccgggcgg
     4141 ggctgcaccg atccgccggg aatagaaaag atcattcttg ttgaagaggt ccgttccctc
     4201 cgtaaaaaaa taccgccgct cgtcgaactt cacctcgaac ggcgaaagat tcagcacaac
     4261 gttgtcgctt tgggtttgcc cgaaatcggg gatcagcgtc atgtcgagcg tgaaactttc
     4321 gttgatgccg tatttgatgt ccatcccgcc gttgagggaa taggcgtttc ttcccgcgta
     4381 attttccgca tacgcgctca catacggaag cagcgcaagg cgtaccggcg cgacaatgtt
     4441 gttgatgccg gcgaggtcgc cgcactggtt cacgacgcct gtcacgttcg ggtcgacgaa
     4501 attccaccac gactgttccc ggtaacggcg gatgatgcgg gtaaagttga gtccccacag
     4561 ctggtcgtcc ccgttcctga aacggatcgc ctggtacggg atcttcattt ccaccgtcca
     4621 gcctttcgcg tcgaccttcg ttttcgaata ccagacggaa ttcaggctga ggtcttccgt
     4681 gtcgaatgta taccgcgaat ccgcctgcac gccggccgcg gtgaccgcga agaaaaatgc
     4741 attgcggcgg tcgtggtatg tgtcgagcat caccccgaac gcatcgctgt tgccctggta
     4801 atcgtcgcgg gcgctgagct ggtgaagaat gctgtcggga gacggatcgt acagccgcgc
     4861 accgatgtag atcgcattgt catcgtaaac gacacgtaca tcctcgtcga atgaggaagg
     4921 atttccggga accggtgcgt tctggatgaa ccgttcagta tgcggggctt tgctccagca
     4981 gctgtcatcg aggtcgccgt caatcgccgg ggcaatggct gcgcgcacgg cttccatcac
     5041 gcgcacagga tcctgcgcag cggccgttgt gacaaaaaag aaaaaaacgg gaaggagcag
     5101 ctttctcacg cggtgcaaat ttagcaacca aaagctccgg ctcgaaagcg cccgatgatg
     5161 aagccctgcg cgtgatcaaa gccatgcccc cgggtacgat cccaacgcag ttgccgaaaa
     5221 aggcaattac aaagacggca ggaaagaagg ctggtggttt tccttccgtc ccggcggaac
     5281 gctgaaagat cctgccggtt acattgacgg aatgccgcag aaacaacctt tgcttttatc
     5341 agaatttcag gctggccggc cggcctttca ttaatcagac gtccaatatt atccctgttt
     5401 tattaattat aaaaagacac taaaccgaat aacagacatc caataatcac cccgactttg
     5461 gtcggctgca gctggcgctg ccttcccaaa taagatactt ttgcgccacc aaaacccact
     5521 tttccacgat gaaccgctcc ttttcattga cggcgaaacc tgttaaccag gatcagtcat
     5581 ggcctgcgaa ggagctttgc tttccgcttt tcaatcaaca cacattttaa ccgatcacag
     5641 gatgaaaaaa attcttctcg cctccgttct gatcttttcc ggcttcttta gcggacgact
     5701 tcacgcacag atgacgacgt tccggttcag ctacgacgta ggactgttcg acatttcagg
     5761 cggcatggtg cagaaccctg ccggtgattt cgtcatcgcc gggctgaaca actctttcgg
     5821 tccgtattac ggaaacgcga tcaaagtcga cgcgcaggga aacgtcgtgt gggcaaaagc
     5881 gtacacgggc ggtttcgcga cgagcttcaa cgacatcaag aacgtcagca cgggcggtta
     5941 catcatgacc ggccagtcga ccagcggcgg cggcggcgct cttcttgtcc gcatcgacgc
     6001 gaacggcaac gtgctctggg cgtatcgcta ccagcttcct gatattcctt cgggaaatgc
     6061 gtccaacgaa tacggtaacg ccgttatcga aacttccgac ggaggatttc tcgtcggcgg
     6121 cggcgtggat tatttctggg acggcgtgag cgcaaacacc gtcgacacga cgagcgcgat
     6181 gggattcaaa gtgaattcaa gcggaacgtt gcaatggagc aaggtgtgga cgatcgccac
     6241 agccaacccg gatgagcatt acatcaacga cgtcgcggaa tcagccgatg gttatttttt
     6301 cgtcgggcaa tcttccgaag gaagcggcac gctgagtagc aacggcgatt acccgagcaa
     6361 cgcgctgatc atcaaaacaa cgacggccgg cgcgctcacg tacatcagaa gatggggagc
     6421 gggcaacacg tcatcacagg ggatcaactg cgccatcacg ctttccacgg gcaatattct
     6481 cctcggcgga ttcgacgacg tgaacgcatt tatcgtcagc atcagcggta ccggttcttc
     6541 cacacctacc gtcattttca ataaacgcct taacggaagt attttcggga atacgttcct
     6601 gctcgaggac atcatggaaa attccgacgg aaattattcc atgatcggaa cacagctggc
     6661 atttttatcc gtgtctctga acacgatgat cggtaagatc aacagctcca ccggtgcgtg
     6721 gatgttcgga cgaacgtatg cgccgatcgg gctttccgcc attctcccgg aaggcggtct
     6781 ttgctccgac cagggttact tcgtttcgat gaccgaccag caggcggccg gcttcaacta
     6841 caacgtcatc cgcaccaata cgagcgggca gacaaacgat cccgccagcg gttgcccggg
     6901 cacaagcatt acgcccactc tcggttcgga atccgtcacg ttctccacac ccaccagctc
     6961 caactatgac ctcatgacgt cgtcgacgtg gacgccggtt gtgcagaacc tcacgcccac
     7021 gcgcacgcag cattgctaca acgcaccgac tccgctgagc acgacatcca cttcaacgaa
     7081 cgtcacctgt ttcggtggat gtaacggaac ggcgactgcg aacccttccg gcggaactgc
     7141 gccgtacacg tattcgtgga actccactcc cgtgcaaacc acgcagacgg caaccggcct
     7201 ctgtccgggc agctacacat gtaccgtaac cgacaatgcc agcgcaacga cgactgtcac
     7261 cgtgaacatc acgcaacctg tttccgcgct gagtgcaagc attccctccc cgacgaacgt
     7321 tacgtgcaac ggctcctgca acggaagcgc cacggtgaat gcaagcgggg gaacttccgg
     7381 ctacacctac tcgtggaact ccgcgcccgt gcaaaccact gcgacagcct ccaatctttg
     7441 cgcaggaagc tataccgtca ccgtcacgga tgcgaacagc tgcacgacca cgcagtccgt
     7501 cacgatcaca caaccatcag cgatcaccgc atctgcgaca tcaacacctt cgagctgctc
     7561 ttcgaataca ggaaccgcca ccgtcagtgc gggcggcggc gcaggcgggt atacgtacag
     7621 ctgggcgcca tcgggcggaa ccggcgcgac ggcgaacaat cttgcaccgg gcaattacac
     7681 ggtaactgtt catgatgcga acaactgcac gcagacagca acggtcaacg taacgacaac
     7741 cacgggtccg agcgcatcgc tgcagtcgca gagcaacgtt tcctgcaacg gcggaaccaa
     7801 cggaagcgca accgtgaatg ccagcggcgg ttcgccggga tacagctact cctggacgag
     7861 cggcggatca tcagcaacgg aaaacaatct tcccgccggg aattacacct gcaccatcac
     7921 cgacgccgca ggttgtgtaa caacacaatc cgtagcgatc accgaaccgt ccgttctttc
     7981 cccgtcttcc acaacatcac ccaccggctg cacgagcagc ggaagcgcaa cggaaacacc
     8041 ttcaggcgga acttcaccct atacgtacgc atggtcttcc ggcggaaccg cagcgacaga
     8101 atcgaatctc ggcgtgggaa cttacacgtg
//
LOCUS       DASTSF010000296         4512 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_43393,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000296 DASTSF010000000
VERSION     DASTSF010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4512
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43393"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            172..831
                     /locus_tag="VFU15_08595"
     CDS             172..831
                     /locus_tag="VFU15_08595"
                     /inference="COORDINATES: protein motif:HMM:NF027718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin-like domain-containing protein"
                     /protein_id="HEU4717880.1"
                     /translation="MKKNLFVIASVAILGGVLTFSSCKKEDTTAPTLSITGGNDQTFS
                     LNSSFTNPTATATDDQDGDVSTKISVTGTVDANTKGNYTLTYSVSDAAGNTASQDVTI
                     HIVNDAEFLAGTYAADDTCQVSWVSPYTMSMSTSNTVNDAITVNNLSNFGTGINLNAT
                     VNISGSSITFSAPQTIGSLGSLNSGSGTYTHNGTAVNANLVWQWTDGTNTEVCTTTAS
                     K"
     gene            895..1233
                     /locus_tag="VFU15_08600"
     CDS             895..1233
                     /locus_tag="VFU15_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015030054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor"
                     /protein_id="HEU4717881.1"
                     /translation="MKNRKSAGGLVYSTNPDFKAESGGEDEAETPAPQQQELRIHLDR
                     LGGGKMVSRVTGFTGRTADLEALGKQLKTKCGVGGSVKDGEILLQGDHRDKIMTVLAA
                     AGYKSKKSGG"
     gene            1259..2332
                     /locus_tag="VFU15_08605"
     CDS             1259..2332
                     /locus_tag="VFU15_08605"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEU4717882.1"
                     /translation="MSAQLPFKGLKVIELANVLAGPSVGMFFAELGANVVKIENPRAG
                     GDPTRKWKLPSEDPSSPVSAYYLSVNYGKQVVFLDLTAPPERERFFALLADADVLIVN
                     FRPGDEVKLGLQYETVSRLFPQLIYAHITGYGPNDNRPAFDLILQAESGLMNINGTPE
                     SGPLKLPVALIDILAGHQLKEGILTALLQRKQGGKGCHVEVSLADAAFTSLINQGTNY
                     SVAGVDPERKGSLHPNIAPYGETFRLKDGNHIVLAVGTEKQFRKLCEALSLETLLTDE
                     MFSTNTARVQNRRKLAEILSASLAVLDFNTFSGKTNGEVPFARIHSVGEAIESEAGKR
                     NLIGMDAEGHHYRVLRSCVFKIS"
     gene            2411..3313
                     /locus_tag="VFU15_08610"
     CDS             2411..3313
                     /locus_tag="VFU15_08610"
                     /inference="COORDINATES: protein motif:HMM:NF039345.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6089 family protein"
                     /protein_id="HEU4717883.1"
                     /translation="MLLLLIPFFCSAQKNKKYRWEVGLNVGASNFLGDLGGANQIGTH
                     FVRDLEPVMTRPAVMATVRYKKDRWLGYRANLGYGKVMGDDKLTQERFRNNRNLNFRS
                     NIFEFSAQVEFYISKERPGHVYHYKKLKGWRHLDIQEYAFVGVGGFYFNPYGKYIDGR
                     WYALRQFRTEGEGIKPGTKMYSRYSVCFPMGMGFKYALNRQWSIGLEFGLRMTLTDYI
                     DDVSTVYFDKTQIAAAQNEPFKQAQAAYFADPSLGLIPTVDGISVTGMNQERGHSDHK
                     DSYIFLQVTVNYKIGKIRKTHSKF"
     gene            3319..3753
                     /locus_tag="VFU15_08615"
     CDS             3319..3753
                     /locus_tag="VFU15_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007657364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU4717884.1"
                     /translation="MRVHEPVTPEDFQNYYRLRWEILRRPWNQAEGSEKDNAEETSFH
                     AMITDDETGECLAVGRLQKNSGSEGQVRFFAVKTEKQGKGLGKMLMSFLEQKAKQLGI
                     ETVILQARENAVPFYLASGYTIREKTFLLFDEIQHYLMEKKI"
     gene            complement(3754..4320)
                     /locus_tag="VFU15_08620"
     CDS             complement(3754..4320)
                     /locus_tag="VFU15_08620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717885.1"
                     /translation="MSEIKNISGRASFVKTEAETSIVITSGAENGKKRITGIILLLWI
                     IGGLYVVINLFTIKQNNTMLVVVIWVAFWIYFAYVMGKSWMWLRKGREVMKIRDGKLF
                     YKRDVGGRGWVHEFAVDRIRNLRPAVSKNPAWLKQFGGDYWSTDCDSMVFNYDDREIA
                     FGFRLDEKEMKKISQLILGTPKKRPEKD"
     gene            complement(4317..>4512)
                     /locus_tag="VFU15_08625"
     CDS             complement(4317..>4512)
                     /locus_tag="VFU15_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014680567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEU4717886.1"
                     /translation="AVVEVAWIRTQGFFAWLIWMFVHLLSLVGFRNRVIVLFNWIVSY
                     INYDRGIRLIIRPFKRQNDS"
BASE COUNT         1160 a         1215 c         1126 g         1011 t
ORIGIN      
        1 ggttacgaat gagagaactt ttttcatctt ggttttttga gtttggttaa ccgggcgcaa
       61 atgtattaat ctttatttat ttaccaaaaa attttgatcc aatgaccatt acgtttactt
      121 ttgcacgaca tttgtataga acccaacaaa acatcttaaa catcaaccaa catgaaaaag
      181 aatctctttg tcatcgcctc tgtggcaatc ctcgggggag ttctcacgtt ctcttcctgt
      241 aagaaagaag atacgacagc tcctacgctc tccatcacgg gcggaaacga tcagacgttt
      301 tctctgaact cttcctttac caacccgact gccactgcta cggatgacca ggacggcgac
      361 gtttcaacca aaatctctgt aaccggaacc gtggatgcca acaccaaagg aaattacacc
      421 cttacctaca gcgtttctga cgcagccggc aatacagcca gccaggatgt gaccatccac
      481 atcgtgaatg acgcggaatt cctcgcagga acctacgctg ctgatgatac ctgccaggtg
      541 tcctgggtca gcccgtacac gatgtcaatg tctaccagca acacggtcaa tgatgccatc
      601 acggtgaaca acctttccaa cttcggaaca ggcatcaacc tgaatgcaac cgtaaacatc
      661 tccggcagct cgatcacctt ctctgctccg cagaccatcg gatcactcgg ttcgctgaac
      721 agcggaagcg gaacgtacac gcacaacggt acggctgtta atgccaacct cgtatggcag
      781 tggacagacg gaacaaatac ggaagtctgt acaacgactg cgtcgaagta atctcttctg
      841 catccttacc aaaaacccgt cagcgatggc gggtttttct tttacctttc gtcaatgaaa
      901 aacaggaaaa gcgcgggcgg gctggtttac tccaccaacc cggattttaa agcggaaagc
      961 ggcggtgaag acgaggcgga aacgccggct ccccagcagc aggaactgcg cattcatctc
     1021 gatcgccttg ggggcggaaa aatggtgagc cgcgtaacgg gattcaccgg caggacagcc
     1081 gacctggagg cgcttggaaa acagctgaaa acgaaatgcg gcgtgggcgg aagcgtgaag
     1141 gacggcgaaa tactcctcca gggcgaccac cgtgataaga tcatgacggt ccttgccgcg
     1201 gcaggataca aatcgaaaaa atcaggtgga taagccgcag gctcggcaac cgggaaacat
     1261 gagcgcacaa cttcctttca agggactgaa agtgatcgag ctggccaacg tgctggcggg
     1321 cccgtccgtg ggaatgttct tcgcagaact gggcgcaaac gttgtaaaaa ttgaaaaccc
     1381 gcgcgcaggc ggcgacccta cccggaaatg gaagctgcct tcggaagatc cctcctcccc
     1441 cgtttcggct tattacctgt ccgtcaatta cgggaaacag gttgttttcc ttgacctgac
     1501 cgcaccaccg gaaagggaac gttttttcgc tcttctcgct gatgccgatg tgctcatcgt
     1561 caatttcagg cccggtgatg aagtaaagct cggattgcaa tatgaaaccg tttctcgttt
     1621 atttccgcaa cttatctatg ctcacataac aggatatggt ccaaacgata acaggccagc
     1681 atttgacctg attctacagg cagaatccgg gctcatgaac atcaacggaa ctcccgagtc
     1741 aggcccgctc aagctgcccg tggcccttat tgacatactg gccgggcacc agctgaagga
     1801 aggcatcctt accgcattgc tgcagcgaaa acagggcggg aaaggctgcc acgttgaagt
     1861 ctcgcttgcc gatgcggctt tcacttcgct catcaaccag gggacgaatt actccgtagc
     1921 gggtgtcgac ccggaacgaa agggctcatt gcatccgaac attgcccctt acggcgaaac
     1981 attccggctg aaagacggca atcacattgt tctggctgtc ggaacggaaa agcaattcag
     2041 gaagctgtgc gaagcacttt ctctcgaaac actgctgacc gatgaaatgt tcagcacgaa
     2101 taccgcgcgc gtgcagaacc ggcgaaagct cgctgaaatc ctttccgctt ctctcgcggt
     2161 tttggatttc aatacatttt ccggcaaaac gaacggagaa gtcccttttg cccgcattca
     2221 ttcggtcgga gaggcgatcg agagcgaggc cgggaaacga aatctcatcg ggatggacgc
     2281 ggaaggacat cattaccgtg ttctccggtc ctgtgtcttt aagatttcct aataatcacg
     2341 actctccgat gaggaaaaac ttttatattt ttgtctttaa gacccttgga tggtgaggaa
     2401 acattttctt ttgctgctat tgctgatccc gtttttctgc agcgcccaga aaaacaaaaa
     2461 gtaccggtgg gaagtcggtc tgaatgtggg cgcttccaat tttctcggtg atcttggcgg
     2521 ggctaaccag atcggtacgc atttcgtgcg tgaccttgaa ccggtaatga cacgcccggc
     2581 ggtgatggcc actgttcgtt acaagaaaga ccgctggctg ggttacaggg cgaacctggg
     2641 ttacggaaaa gtaatgggag atgacaagct tacgcaggaa cgtttccgta acaaccgcaa
     2701 cctgaatttc agatccaaca ttttcgaatt ttcggcacag gtcgaatttt acatcagcaa
     2761 ggaaaggccc ggtcacgttt atcattacaa aaagctgaaa ggctggcggc acctcgatat
     2821 ccaggaatac gcattcgtgg gtgtcggcgg attttatttc aatccttacg gaaaatacat
     2881 cgacggcagg tggtatgccc tgcgccagtt ccgcacggaa ggcgaaggaa taaagcccgg
     2941 aaccaaaatg tattcccgct acagcgtgtg cttcccgatg ggaatgggct tcaagtacgc
     3001 cctgaaccgc cagtggagca tcgggcttga attcggtctg cgcatgacgc tcaccgatta
     3061 catcgacgac gtcagcacgg tgtattttga caaaacgcag atcgctgcgg cgcagaatga
     3121 acctttcaag caggcgcagg ccgcctattt cgccgatccg agtctcgggc ttatcccgac
     3181 ggtcgacggc atcagcgtaa cgggcatgaa ccaggagcgc gggcattccg accacaagga
     3241 ttcctacata ttcctgcagg tgacggtcaa ttacaagatc ggtaagatcc gcaaaacaca
     3301 ctccaagttc tgacgcgtat gcgcgtgcac gaacctgtta ccccggaaga ttttcagaac
     3361 tactacaggc tgcgctggga aatattgcgc aggccgtgga accaggccga aggttcggaa
     3421 aaagacaacg cggaagaaac ttcttttcac gcgatgataa cggacgacga aaccggggaa
     3481 tgccttgcgg tgggccgcct gcagaaaaat tccggcagcg aaggacaggt gcgtttcttc
     3541 gccgtgaaaa ccgaaaagca gggaaaaggt ctcggaaaaa tgctgatgag cttcctggaa
     3601 cagaaagcaa agcagctcgg aattgaaacc gtcatcctgc aggcgaggga aaatgcagtt
     3661 cccttctatc tcgcttccgg ttacacgatc cgcgaaaaaa catttcttct cttcgacgag
     3721 atccagcatt acctgatgga gaaaaagatc tgatcaatcc ttttccggcc tcttcttcgg
     3781 cgtgccgagg atcagctgcg atatcttctt catttccttt tcatccagcc ggaaaccgaa
     3841 ggcaatttcc cggtcatcgt aattgaacac catcgaatcg cagtcggtcg accagtagtc
     3901 gccgccgaac tgcttcagcc atgctggatt ttttgaaacc gccgggcgca ggttacggat
     3961 acggtcgacc gcaaattcat gcacccatcc tcttccgccc acgtcgcgct tgtagaataa
     4021 tttcccgtcg cggatcttca tcacttccct gcccttgcgc agccacatcc atgattttcc
     4081 catgacgtac gcaaaataga tccagaacgc cacccagatc acgacaacga gcatcgtatt
     4141 gttttgtttg atcgtgaaca ggttgatcac gacgtaaagt ccgccgatga tccagagcag
     4201 cagtatgatc ccggtgatcc gcttttttcc gttctccgct cccgaagtga tgacaatact
     4261 cgtttccgcc tccgtcttca cgaaagaagc ccgtccgctg atgttcttta tttcactcat
     4321 gaatcatttt gtcttttgaa cggacggatg atcagccgta tcccgcggtc gtagttgatg
     4381 tagctcacga tccagttgaa cagcacgatc acgcggttcc tgaaaccgac aagggaaagg
     4441 aggtgcacga acatccagat cagccaggca aaaaagccct gcgtgcggat ccacgcgact
     4501 tccaccaccg cc
//
LOCUS       DASTSF010000297         8239 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_4769,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000297 DASTSF010000000
VERSION     DASTSF010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 8239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8239
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4769"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1024)
                     /locus_tag="VFU15_08630"
     CDS             complement(<1..1024)
                     /locus_tag="VFU15_08630"
                     /inference="COORDINATES: protein motif:HMM:NF039534.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717887.1"
                     /translation="MKRSLLNSCLAVLAPVFMAGSLSAQCSAVTPTTTNVSVSCGGVA
                     TLTASGSAGYQWWDQSSGGNMVGTGSSYSTGPLTADTNLYVEGTATSPTTLMGLPTYN
                     ATFTGNVRGYWFTAPVDFTITGLDIPTNVSGTQNIAVVKLTAAPPLYSSTTNAFTTLF
                     LTQNDPTVGTIPVNIPIAAGDIIGILGQAGSNNAYAPANYNTTIDGQPVTLARLGMQY
                     PLNTTAPQELWTETTGSISLVEMYYTTSCTSPRVQVGVTVTPIPVGASAVNPALCAGD
                     STVLNATGATNYTWMPGNLTGASITVTPSATTTYTVTGDDGGMCSNTATVTVTVNPLP
                     TISASAS"
     gene            1355..2743
                     /locus_tag="VFU15_08635"
     CDS             1355..2743
                     /locus_tag="VFU15_08635"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEU4717888.1"
                     /translation="MKKVFLFLILAIPFAAKAGGPVITGGIFPQVIEGSSPTNNNRVE
                     FWCWVNIGGLNANATYHYFTAMDTVGTPLTGNGAGLPVLINPTTGSIRRILNPSMTAN
                     TGYDSITASATGTYSGWFGVEPTGNFRFTPGTMLDFILMLNNGSGGTTVSYRLAASAF
                     PITVMNFGIMSASPTQGSAVYDSLDAAPRNFICLYDNTTATGRPVNIAIVEDDSLYLR
                     PIASIAAFYRNRVDSLPMHWGALIPNNLPNGIRALEERDFLTGMPIDTSMDADGIWCS
                     GLNTVNMSSGNTGKFLNSTFMLSDTASIPDTTYTNFPTGFTASSNSPVATYMWNFGDT
                     GTASTASASHTYLSPGVYLVQVVISTGGCSDTITHTVVVLLSTFIPKAPTLWFGVTPN
                     PATDYISISTRDNDNKTLRMYDILGETILTRSYSGNNLMLDLAGQQPGIYFLEIKDEV
                     TGRTGLKKIIIQ"
     gene            2873..4255
                     /locus_tag="VFU15_08640"
     CDS             2873..4255
                     /locus_tag="VFU15_08640"
                     /inference="COORDINATES: protein motif:HMM:NF026253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HEU4717889.1"
                     /translation="MKPFFLSCFAVFFFISVHAQTGAHPAAAPKSPASDTWKPFLSNG
                     KWGYKDTTGKVVIQPKLNFLWCYHFSEGFAVVQNNDYTYSYINTTGVNSFNKSFEFAQ
                     DFSDKMAAVMENETFGYINTTGQYAFPDKFYEVYPYHEGFASVKKGGKYFYINKQGKN
                     QFGQTYSDLNAFSEGFAAVNKTGKKWSYIDKTGKDAFNGQEFDDAGDFSSGYAQVLTG
                     GRYHFIDHNGKNAFDQEFDIAYHYAFGYAVVKKDDESGYYFIDNTGKNRFGGTYDFVD
                     GYQDGAAVAERNDKYYYIDTSGHRIRGGKAFDDCYAYHYGIIPVKEGGSWHYIDENGK
                     DPFDRDFDRAYQFGNGIALIMNNDKFYFIDETGQNPFGQTFDDASEFTHGFARVTRGD
                     SVFFIDRRGKIAFGNFEEVNDDGLPFSNYYVKAKQNGKWGLINLQGKPVIEFRYSDPD
                     NCPAKLPDNE"
     gene            complement(4364..6151)
                     /gene="lepA"
                     /locus_tag="VFU15_08645"
     CDS             complement(4364..6151)
                     /gene="lepA"
                     /locus_tag="VFU15_08645"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HEU4717890.1"
                     /translation="MKNIRNFCIIAHIDHGKSTLADRLLEFTKTVSARDMEAQVLDDM
                     DLEKERGITIKSHAIQMTYTLNGEQYALNLIDTPGHVDFSYEVSRSIASCEGALLIVD
                     AAQGIQAQTISNLYLALGNDLEIIPVLNKIDLPSAEPEVVKDQIVDLIGCKREDIIAA
                     SGKTGFGVTDILAAIIERIPSPKGDPSAPLQALIFDSVFNSFRGIIAYFKVVNGEIRK
                     GDKVKFFNTGSEYIADEVGILRLSLEPRDAVKTGDVGYIISGIKDAREVKVGDTITQV
                     ENPCREGIRGFEDVKPMVFAGIYPVDTDDFEELRYSMEKLQLNDASLTWEPESSAALG
                     FGFRCGFLGMLHMEIVQERLEREFNMTVITTVPNVSYIAYMTSGEVINMHNPSDMPDV
                     VKIDYIEEPFIKANIITKAEYVGAIIKLCMEKRGTFVNQQYLTTDRVEMVFEMPLGEI
                     VFDFYDKLKSISKGYASFDYHQIGYRQSDLIKLDILINGDQVDALSALIHRSRAHEFG
                     KKICEKLKELIPRQQFQIAIQAAIGAKIVARENISAMRKDVTAKCYGGDISRKRKLLE
                     KQKEGKKRMRQIGSVEVPQSAFMAVLKLD"
     gene            6415..7164
                     /locus_tag="VFU15_08650"
     CDS             6415..7164
                     /locus_tag="VFU15_08650"
                     /inference="COORDINATES: protein motif:HMM:NF014626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HEU4717891.1"
                     /translation="MCYHYSLSISSEELIKRYSAFDEDYALLQELYGEIHYYLNGFSH
                     PLVPVVVHEKQDRLKLSRWGLIPSWTRSREDALEIANKTLNARSESVFEKASFRESIL
                     KRRCLVPATGFFEWQAVKGKKYPHFISLKSEKIFSFAGLYDEWVDRSSGEIVHSFSLL
                     TTEANPLMAKIHNTKKRMPLILPSEKEKLWLDETLSRETLTQLFAAYDENDMSAYTIS
                     RLVSSGEEKDTPAVLAAHTYPELQTQQGELF"
     gene            complement(7229..8173)
                     /locus_tag="VFU15_08655"
     CDS             complement(7229..8173)
                     /locus_tag="VFU15_08655"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEU4717892.1"
                     /translation="MKKLLLLLAATPAFLAGQSIPNPGFETWVSNSESPHTYQTPQGW
                     ITGDIIQSSFSNLFGDPAYVSNTVSQVSSAHGGSSAVQMTVAQSNEGDTVAGIIYSVA
                     SANTVISSAFNGTPAMGFPYTTRSANLTGYYKLTRMGGDTAGAAICFTKWNTATQMRD
                     TVALVNLFPVLTSASSWTSFTIPVNYLTSEYPDTAFIAFGISSATPHMGTTFTIDDIA
                     FSGTVPAGIDEQHADNSLVSLFPNPFSDQATLDISKLNIDHARLEIYDMLGNKVRVMD
                     DITGDKVVIDREGLPSGVYFYYLMNGNALAAEGKISVQ"
BASE COUNT         1956 a         2200 c         2228 g         1855 t
ORIGIN      
        1 tcgaggcgga agcggaaatg gtgggcagcg ggtttacagt taccgtcact gttgctgtat
       61 ttgaacacat cccgccatcg tcgcccgtaa cggtgtacgt tgtcgttgcg gaaggagtca
      121 ccgtgatgga agcgccggta agattgccgg gcatccatgt gtagttggtt gcgcctgttg
      181 cgttcagcac agtggaatca ccggcgcaga gcgcgggatt aaccgcagaa gctccgacgg
      241 gaatgggagt gactgtgaca cccacctgca cgcgcggact ggtgcaggaa gtggtgtaat
      301 acatttccac gagcgagatc gaaccggttg tttccgtcca cagttcctgc ggagcagtgg
      361 tgttgagcgg gtactgcatg cccaggcgcg caagagtgac gggttgcccg tcgatcgttg
      421 tgttgtaatt ggccggagcg taggcattgt tgctgcccgc ctgtcccagg atgccaatga
      481 tatcgccggc cgcaatggga atattgacgg gaattgttcc caccgtggga tcgttctgcg
      541 tcaggaaaag cgtggtgaat gcgttcgtgg tactggaata cagcggaggt gctgccgtga
      601 gcttcacgac ggcaatgttt tgcgtgccgc tgacattcgt cggaatatcc agcccggtga
      661 tggtgaaatc gaccggggcg gtgaaccagt atccgcgtac gttccctgtg aatgtggcgt
      721 tgtaggtcgg caatcccatg agggtcgtcg gtgaggtggc cgttccttcc acgtaaagat
      781 tggtgtctgc agtgagcggg cctgttgaat agctggaacc ggttcccacc atgtttccgc
      841 cgctcgactg gtcccaccac tggtaaccgg cagagccgct cgcagtaaga gttgcaacgc
      901 ctccgcagct cacggaaacg ttcgttgttg tcggcgttac ggccgaacat tgtgcgctca
      961 gcgagccggc cataaagacc ggcgcaagaa cagcaagaca ggaattgagt aaacttctct
     1021 tcatttctga tttgatttaa atgaaaaaca tccgcccggg gaaaaccatg gactacatga
     1081 gaatattaac gggaagattt ttatttgcct ttgctaaaat atcgaaaact atctctttcg
     1141 gagaccattc accggctttt gtccggaacg gcgactgttg gatactgtat atcaggcgct
     1201 aaatgtgcga aacggaagaa cacgacggcc ctggaaaagg ggaaatcaac aatttgctat
     1261 taacaactcc gatttgcttc accctgggcg ggaaagggct tttctaattt tgctgaacaa
     1321 aagcaaaatt tttgctcaat aaaccgaaag ctccatgaaa aaagtattcc tgttcctgat
     1381 ccttgcgatt ccttttgcgg cgaaagccgg agggccggtc atcacggggg gaatatttcc
     1441 ccaggtcatc gaagggtctt ccccgacgaa caataaccgt gtagaattct ggtgctgggt
     1501 gaatatcggc gggctcaacg cgaacgccac ctatcattat ttcacggcaa tggataccgt
     1561 tggcaccccg ctcacgggca acggcgccgg gcttcctgtt cttatcaacc cgaccacggg
     1621 ctcgatccgc aggatcctga acccgtcgat gacagcgaat acaggttacg actcgatcac
     1681 cgcgagcgcc acaggaacat attcgggctg gtttggcgtt gagccgacgg gaaatttcag
     1741 gtttacaccc ggaacgatgc tggatttcat tctcatgctg aacaacggct ccggcggaac
     1801 aaccgtttct taccgcctgg ccgcttctgc cttccccatt accgtaatga atttcggaat
     1861 tatgtcggca agcccgacgc agggttccgc ggtttacgat tcactggacg ccgccccgcg
     1921 aaatttcatc tgcctgtacg acaacaccac ggcaaccggc cgcccggtga acatcgcgat
     1981 cgtcgaagat gattcacttt acctccggcc catcgccagc attgccgctt tctaccgcaa
     2041 ccgcgtcgac tctcttccca tgcactgggg cgcattgatc ccgaacaacc ttcccaatgg
     2101 tatccgcgcg ctcgaagaac gcgatttcct caccggcatg cccatcgaca cgtcgatgga
     2161 tgcggacggc atctggtgct ccggcctcaa tacggtgaac atgtcttccg gcaatacggg
     2221 aaaattcctg aactcgacgt tcatgctgtc ggatacggct tcgatacccg acacgaccta
     2281 taccaatttc ccgacgggct tcaccgcgtc ttcgaacagc ccggttgcta cgtacatgtg
     2341 gaacttcggt gacaccggga ccgcgtccac ggcttctgct tcgcacacgt atctttcccc
     2401 gggcgtttac ctcgtacagg tagtgatttc caccggcggc tgcagcgata cgatcacgca
     2461 taccgtggtt gttctcctca gcacattcat cccgaaagcg cccacactgt ggttcggtgt
     2521 aacaccgaat cccgccaccg actacatcag catctccacg cgcgacaacg acaacaaaac
     2581 actgcgcatg tatgacattc tcggcgaaac gatcctcacg cgcagctaca gcgggaacaa
     2641 cctgatgctc gaccttgcag gacagcagcc cggaatttac tttcttgaaa taaaagacga
     2701 ggtgacggga agaacggggc tgaaaaagat cattatccag taaatccatg gtttcaaaaa
     2761 gatggaaggc tgttccgcaa gggacggcct ttctgttttt gcaaacagca gcagcccgca
     2821 gcgtgaacca aaaaaaaatg taaaatagct gaacaaaaaa gagccctgtc ctatgaaacc
     2881 cttcttcctt tcctgctttg cagtattttt tttcatttcc gttcacgcgc agaccggcgc
     2941 acacccggca gccgcgccga aatcacccgc atcagatacg tggaaaccat ttctctccaa
     3001 tggaaaatgg ggatacaaag acaccaccgg gaaagtggtc atccagccga agctgaattt
     3061 cctctggtgc tatcattttt cagaaggctt cgccgtggtg cagaataatg attacacgta
     3121 ttcctacatc aacaccaccg gcgtgaacag cttcaacaag tcgttcgaat tcgcgcagga
     3181 tttttccgac aagatggcgg cggtgatgga aaacgaaacg ttcggctaca tcaacacgac
     3241 cggccagtac gcattccctg ataaattcta tgaagtttac ccctatcacg aaggatttgc
     3301 gtcggtgaaa aaaggcggaa aatattttta catcaacaag cagggaaaga accagttcgg
     3361 gcaaacgtac agcgacctca atgcattttc cgaaggattt gcggcggtga ataagaccgg
     3421 gaaaaaatgg agctacatcg acaagacggg gaaagacgct ttcaacggcc aggagtttga
     3481 cgatgcgggc gatttttcaa gcggctacgc acaggtgctg acaggcggcc gctatcactt
     3541 catcgatcac aacgggaaaa atgcgttcga ccaggaattc gacatcgcgt atcattacgc
     3601 tttcggttat gccgtggtga aaaaagacga cgaaagcggc tattacttca tcgacaacac
     3661 cgggaaaaac cgtttcggcg gtacgtatga tttcgtagac ggctaccagg acggcgctgc
     3721 agtagcagaa cgtaacgaca agtattacta catcgacacg agcgggcacc gcataagagg
     3781 cggcaaggcg ttcgatgact gctacgccta tcattacgga attattcccg tgaaagaagg
     3841 cggaagctgg cattacatcg acgagaacgg gaaggatccg ttcgaccgcg atttcgacag
     3901 ggcttaccag ttcgggaacg gcattgccct catcatgaat aacgacaaat tttatttcat
     3961 tgacgaaacc gggcagaatc cgttcggcca gacgttcgac gatgccagcg aattcacgca
     4021 cggctttgcc cgcgtgacgc gcggcgacag cgtattcttc atcgaccgcc ggggaaaaat
     4081 tgcgttcggc aacttcgaag aagtgaatga cgacgggctt ccgttcagca attactacgt
     4141 aaaagcgaaa cagaacggca aatggggact catcaacctg cagggaaaac ccgtgatcga
     4201 attccgctat tcggatccgg ataattgtcc tgcgaagctg ccggataatg aatgaccggc
     4261 tttgtctttt tcttccggac aaaagcgcaa cgtgtctgca aaaaattgcc ggaaattcct
     4321 ttcgcgtctt tgcgtgaatt cctgaattaa ccccggacgg ggatcaatcc agcttgagca
     4381 cggccatgaa cgcgctctgg ggaacttcca cggaaccgat ctggcgcatg cgcttcttgc
     4441 cctccttctg cttttcgagc agctttcgtt tccgtgaaat atcaccgccg tagcattttg
     4501 cagtaacgtc cttgcgcatg gcggaaatat tttcacgcgc cacgatcttc gcgccgatgg
     4561 cggcctgtat cgcgatctgg aactgctggc gcgggataag ctctttcagt ttttcgcaga
     4621 tcttctttcc gaactcgtgc gcgcgcgagc ggtgaatgag cgcggaaagc gcatccacct
     4681 ggtcgccgtt gatgaggata tcgagcttga tcaggtccga ctggcggtaa ccgatctggt
     4741 ggtaatcgaa tgacgcgtag cctttcgaga tggatttcag cttgtcgtaa aaatcgaaga
     4801 cgatctcgcc gagcggcatt tcgaaaacca tttcgacgcg gtcggtggtg aggtactgct
     4861 ggttcacgaa cgttccgcgt ttttccatgc acagcttgat gatggcgccc acgtattcgg
     4921 ctttcgtgat gatgttcgcc ttgatgaacg gttcttcgat gtaatcgatc ttcaccacgt
     4981 ccggcatgtc gctcgggttg tgcatgttga tcacctcgcc cgacgtcatg tacgcgatgt
     5041 acgacacgtt gggcacggtc gtgatcaccg tcatgttgaa ctcgcgctcg agacgttcct
     5101 gcacgatctc catgtgcagc atgccgagga atccgcagcg gaacccgaaa ccgagcgcag
     5161 cggaagattc cggctcccag gtaagcgacg catcattgag ctgcagcttc tccatggaat
     5221 agcgcagctc ctcgaaatcg tccgtatcga cgggataaat tccggcgaag accatgggct
     5281 ttacatcttc gaatcccctg atgccttcgc ggcacgggtt ttccacctgc gtgatcgtgt
     5341 cgcccacttt cacttcgcgc gcatccttta tcccggaaat gatgtagccc acgtcaccgg
     5401 ttttcacggc gtcgcgcggc tcgaggctga ggcggagaat tcccacttcg tcggcgatgt
     5461 actcgctgcc ggtattgaaa aatttcactt tgtcaccttt gcggatctca ccgttcacca
     5521 ctttgaaata cgcgatgatg ccgcggaagg aattgaacac ggaatcgaaa atgagcgcct
     5581 gcagaggtgc agaaggatca cctttgggcg aaggaatgcg ttcaatgatc gccgccagaa
     5641 tatcggtgac gccgaacccg gttttcccgg atgcagcaat gatgtcttcc cgtttgcagc
     5701 cgatgaggtc cacgatctgg tctttcacca cttcaggttc cgcactcggc aggtcgatct
     5761 tgttgagcac gggaataatt tcgagatcgt ttcccagggc gagatacaaa ttggagatcg
     5821 tctgcgcctg tatgccctgc gccgcatcga caatgaggag cgcgccttcg caggaggcga
     5881 tggaacgaga aacttcgtag gaaaaatcga cgtgacccgg cgtatcgatg aggttcagcg
     5941 catactgttc gccattgagc gtgtacgtca tctggatggc gtggctcttg atggtgatgc
     6001 cgcgttcttt ctcgagatcc atgtcgtcga gcacctgcgc ttccatatca cgggccgaaa
     6061 ccgttttggt gaattcgagc aggcgatcgg ccagcgtgct cttgccgtgg tcgatgtgcg
     6121 cgatgatgca gaaattgcga atgttcttca tggaaaaaaa ccgggacgca aaaatagtga
     6181 aaaaggcatg gcggcggtaa taaggacgaa cccgcctgtt aagtgactat tggttttgcg
     6241 gggagaagaa cttttaccgc agagcaattt aaacgccggc ggcggacaag aaggttcgca
     6301 aagaaggact tattgcaacc ggggttatgt gtctttgcgt gaaaagactt ttccggggga
     6361 aaccacgatt tcctcccggg ttgaataact tgcagccggt tatcttcacc tgccatgtgc
     6421 taccattaca gcctgagcat aagctccgaa gagctcatca aacgttattc tgcgttcgat
     6481 gaagattacg cgctgctgca ggaactctat ggtgaaatcc actattacct gaacgggttc
     6541 agtcacccgc tcgttcccgt cgtggtgcat gaaaagcagg accggctgaa gctttcccgc
     6601 tggggactca ttccttcctg gacaagatcg cgggaagatg ccctggagat cgcgaacaaa
     6661 accctgaacg cacgcagcga atcggtattt gaaaaagcat cattccggga aagcatcctg
     6721 aagcggagat gcctcgtacc cgcgaccggt tttttcgaat ggcaggcggt gaaaggaaaa
     6781 aaatacccgc atttcatctc gctgaaaagt gaaaagatct tttcattcgc cggcctttac
     6841 gatgaatggg tggaccgctc ttcgggcgag atcgtgcaca gcttcagcct cctcaccacg
     6901 gaagcgaacc cgctcatggc aaagatccac aacacgaaaa aacgcatgcc gctcatcctc
     6961 ccgtcggaaa aagaaaagtt gtggctcgac gaaacgcttt cccgcgaaac gctcacacag
     7021 ctgttcgccg catacgacga gaacgacatg agcgcgtata cgatcagccg gctcgtctcg
     7081 tcgggggaag aaaaagacac acccgctgtg cttgcggcgc atacttatcc ggagctgcag
     7141 acacagcagg gcgaactgtt ctgaatgctt ccgcaaacaa aaacgtcctc cctttcggag
     7201 aggacgtccg gggttttgca aaaaagattt actgcaccga tatttttcct tcagctgcca
     7261 aagcgttccc gttcatgagg taatagaaat acacaccgga aggaagtcct tcgcggtcga
     7321 tcaccacttt gtcgccggtg atgtcatcca tcacgcgcac tttgttgccg agcatatcgt
     7381 agatctcgag acgtgcgtga tcgatgttca gcttgctgat gtcgagcgtt gcctggtcgc
     7441 tgaacgggtt cgggaagagc gacacgaggg aattgtccgc atgctgctcg tcgatgccgg
     7501 ccggaaccgt tcctgaaaaa gcaatgtcgt cgatcgtgaa cgtggtgccc atgtgcggcg
     7561 tcgccgagct gatgccgaat gcgatgaagg cggtatcggg atattccgaa gtaaggtagt
     7621 tgaccggaat ggtgaacgac gtccagctgg aagcgctggt taagaccggg aacaggttta
     7681 cgagtgccac cgtatcgcgc atttgcgtgg cagtgttcca tttcgtgaag cagatggctg
     7741 caccggccgt gtcgccgccc atccgcgtca gcttataata tcccgtcagg ttcgccgatc
     7801 gcgtcgtata cgggaatccc atcgccgggg tcccgttaaa cgcagagctg atcaccgtat
     7861 tcgctgacgc gacggaataa atgatgccgg ccaccgtgtc gccctcgttg ctttgcgcaa
     7921 cggtcatctg gaccgcagac gatcccccgt gggcgctgct cacctgggaa acagtgttcg
     7981 aaacatacgc cgggtcgccg aacaggttgc tgaacgacga ctgaataata tcgcccgtga
     8041 tccatccctg cggcgtctgg tacgtgtgcg gactttccga attgcttacc cacgtttcga
     8101 atcccgggtt ggggatggac tgccccgcaa gaaaagccgg ggtggctgcc aaaagaagta
     8161 gaagtttttt catggttaat tttttacaaa tatccggcaa gcaggcggaa ttgcgcaaca
     8221 acatgaaaat gcggtaatg
//
LOCUS       DASTSF010000298         3616 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1798357,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000298 DASTSF010000000
VERSION     DASTSF010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3616
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1798357"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..877
                     /locus_tag="VFU15_08660"
     CDS             <1..877
                     /locus_tag="VFU15_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016776863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HEU4717893.1"
                     /translation="AMDRTEVNALISLLDDPDEHVFSQIREKLLSLGTDVIPVLESAW
                     ENSFDSVLQQRIENIIHKIQFDEIRRSLREWSFPENQDLLSGAILIAKYQYPDLDESH
                     IRRHIEQIKQDIWLELNNNLTALEKIRVINHILFDVHGFSANTTNYHAPQNSYINNVL
                     ETKKGNPVSLSIIYAVIAQDLRIPVYGVNLPGHFILAYVDNGPGFSLDADNTDRVLFY
                     INPFSRGAPVNKKEIDAFIRQQRLDPKPEYYTPCSNLQIIRMLLNNLIASYEKLGYPN
                     KVDELKLLWKALEDF"
     gene            974..1873
                     /locus_tag="VFU15_08665"
     CDS             974..1873
                     /locus_tag="VFU15_08665"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018478121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HEU4717894.1"
                     /translation="MKNITVIGSGTMGNGIAHVFAQFGYNVTLVDISKEAIDKGLATI
                     TKNLDRQVAKGAVTEEQKKETLARISPATDLASAVKNADLVVEAATENPEIKFRLFKD
                     IDANAPAGCILASNTSSISITKIGAATKRPAQVIGMHFMNPVPVMKLVEVIRGYNTSD
                     EVTSVVMDLSRKLGKVPVEVNDYPGFVANKILMPMINEAIITLHEGTAGVEEIDTVMK
                     LGMAHPMGPLRLADFIGLDVCLYILRVLHDGYGNPKYAPCPLLVNMVTAGHLGDKSKK
                     GFYDYADPKSPVVAEQFRKKELV"
     gene            complement(1927..3405)
                     /gene="guaB"
                     /locus_tag="VFU15_08670"
     CDS             complement(1927..3405)
                     /gene="guaB"
                     /locus_tag="VFU15_08670"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEU4717895.1"
                     /translation="MSADSSKIFTEGLTYDDVLLLPSYSEVLPREVDLSTQFTRNIRI
                     NAPIVSAAMDTVTEFQLAIAIAREGGIGVIHKNMTIAQQADHVRRVKRSESGMIMDPV
                     TLSVNSTLADALNLMKEHKIGGIPVTSSDGKLVGIVTNRDLRFEKNLKKPVREVMTAE
                     KIITTKQGTDLKKAEQILQQYKIEKLPVVDKEGKLVGLITYKDIIKIKERPTASKDPL
                     GRLRVAAAVGVTSDTLERADALVKAGADAIIIDTAHGHSKGVIEMLKLVKKTYPSTDI
                     VVGNIATAEAGLALVKAGADAVKVGIGPGSICTTRVIAGVGVPQLSAVMMVAEALKKT
                     GVPIIADGGIRFTGDIPKAIAAGAHTIMAGSLFAGTEESPGETIIYEGRKFKSYRGMG
                     SLEAMQKGSKDRYFQDVEDDIKKLVPEGISGRVPYKGSLAEVIYQLVGGLRAGMGYCG
                     AKNISELHKAKFIRITAAGIRESHPHDIAITREAPNYSRDSY"
BASE COUNT          825 a         1042 c          891 g          858 t
ORIGIN      
        1 tgccatggac cggacggaag taaacgccct tatcagtctg ctggatgatc cggacgagca
       61 tgttttttcc cagatccggg aaaagctgct ctcgctggga acggatgtca ttcccgttct
      121 tgaaagcgca tgggaaaatt ccttcgactc tgttctccag cagcggatcg agaacattat
      181 tcacaagata cagttcgatg agatccgccg cagcctgcgg gaatggtcgt tccccgaaaa
      241 ccaggacctg ctttccggtg ccattctcat tgcgaaatac cagtatcccg acctcgacga
      301 atcgcacata cgccgccaca tcgagcagat caagcaggat atatggctcg agctgaacaa
      361 caacctcacg gcgctggaaa agatccgcgt gatcaatcat attcttttcg acgtgcatgg
      421 attcagtgcg aatacgacga attaccacgc gccgcagaat tcctacatca ataatgtgct
      481 ggagacgaaa aaagggaatc ctgtttcgct ttcgatcatt tacgccgtga tcgcgcagga
      541 tctgcgcatc ccggtttacg gcgtcaacct tccgggacat ttcattcttg catacgtcga
      601 caacgggccc ggtttctcgc tcgatgccga caataccgac cgggtgctgt tttacatcaa
      661 tcctttttcg cgcggtgcgc cggtaaacaa aaaagagatc gacgcattta tccgccagca
      721 gcgtcttgat ccgaaaccgg aatattatac cccctgctcc aacctgcaga tcatacgcat
      781 gctcctgaat aatcttatcg cttcatacga aaagctcggc tatcccaata aagtcgacga
      841 actgaagctg ctgtggaaag cgctggagga tttttgaccg tatgcagttt tttttccggg
      901 ctccaaatat tttccccggc cgctgccata attcatccat cctgttacct ttaccgctcc
      961 aatcatttta ccaatgaaaa acatcacagt cataggctcg ggaaccatgg gcaacggcat
     1021 cgcgcacgtg ttcgcccagt tcggatacaa cgttacgctt gtcgacattt caaaagaagc
     1081 gatcgataag ggactcgcca ccattaccaa aaacctcgac cggcaggtgg ccaaaggcgc
     1141 cgtcacggaa gagcagaaaa aagaaacgct cgcacgtatt tctcccgcaa cggatcttgc
     1201 gtctgccgta aaaaatgcgg atctcgtcgt ggaagccgcc accgaaaacc cggaaataaa
     1261 attcaggctg ttcaaagaca tcgacgcgaa tgctcccgcg ggctgcatcc tcgcttcgaa
     1321 cacatcctcc atttccatca cgaagatcgg tgccgccaca aaacgtcccg cgcaggtgat
     1381 cggcatgcat ttcatgaacc cggttccggt gatgaagctg gtggaagtga tccgcggcta
     1441 caacacgagc gatgaagtga catccgtcgt catggatctt tcccggaagc tcggaaaagt
     1501 tcccgtcgaa gtgaacgatt atcccggttt cgtggcgaac aaaatcctta tgccgatgat
     1561 caatgaagcc atcatcacgc tgcatgaagg aacggcaggc gtggaagaga tcgacacggt
     1621 gatgaagctc ggcatggcgc acccgatggg accgctccgc ctcgctgatt tcatcgggct
     1681 cgatgtttgc ctgtatattc tgcgtgtgct gcacgacggc tacggaaatc cgaaatacgc
     1741 gccctgcccg ctgctggtga acatggtcac cgcgggtcat ctcggcgaca aatcgaagaa
     1801 aggattctac gactacgccg atcccaaatc gccggtagtg gcggagcagt tccggaaaaa
     1861 agaattagtg taaaagaaga aatgagtaaa aaaacaaaga gcgggaaaat tcccgctctt
     1921 tgtttttcag taagaatccc tgctgtaatt cggtgcttcc cgcgtgatgg cgatgtcgtg
     1981 cggatggctt tcacgtatcc ccgctgccgt gatgcggatg aattttgctt tgtgcagttc
     2041 cgaaatattc tttgctccgc aataacccat tcccgcacga agtccgccga ccagctggta
     2101 aatcacctcc gccaggctgc ctttgtaagg cacgcggccg gatatacctt ccggcacgag
     2161 cttcttgata tcgtcctcca catcctggaa ataacggtcc ttcgaacctt tctgcatcgc
     2221 ctcgagcgaa cccatcccgc ggtatgattt gaattttctt ccttcgtaaa tgatcgtttc
     2281 gcccggcgat tcttcggttc cggcaaataa ggaaccggcc atgatcgtat gcgcacccgc
     2341 agcgatcgct ttcgggatgt cgccggtaaa acggatgccg ccgtcggcaa tgatcggaac
     2401 gccggttttc ttcagcgctt cagccaccat catcaccgcg ctcagctgcg gaacaccgac
     2461 accggcgatc acgcgcgtgg tgcagatcga gcccggtcct attcccactt ttaccgcatc
     2521 cgctcccgct ttcacgagcg caagcccggc ttccgcggtc gcaatgtttc caaccacgat
     2581 gtccgtcgaa ggataggttt tcttcaccag cttcagcatt tcgatcaccc ctttcgaatg
     2641 accgtgcgct gtatcgatga tgatcgcgtc ggcgccggct ttcaccagcg cgtccgccct
     2701 ttccagtgta tcggatgtta cgcccaccgc cgctgcaacg cgcaggcgcc cgagcggatc
     2761 cttgcttgcc gtcgggcgtt ccttgatctt gatgatgtct ttgtaagtga tcagtcccac
     2821 cagcttccct tccttgtcca ccaccggcag cttttcgatc ttgtactgct ggagtatctg
     2881 ttccgccttc ttgaggtcgg ttccctgctt cgtggtaatt attttttccg cggtcatcac
     2941 ttcccttacc ggcttcttca ggttcttctc gaaacgcagg tcgcggttgg tcacaattcc
     3001 caccagcttc ccgtcagaag acgtaaccgg gataccgccg atcttgtgtt ctttcatcag
     3061 gttcagcgca tccgccagcg tggaatttac gctcagcgtc accgggtcca tgatcatccc
     3121 gctctccgat cgtttcacgc gacgcacatg atccgcctgc tgcgcaatcg tcatgttctt
     3181 gtgaataacg ccaatcccgc cttcgcgcgc aatagctatt gcaagctgga attccgtcac
     3241 cgtgtccata gcggccgaca cgatcggcgc attgatgcgg atgttgcgcg tgaattgggt
     3301 ggaaagatct acttcgcggg gaagaacttc agaataggag ggcaaaagaa gaacgtcgtc
     3361 gtaagtgagt ccttcggtga aaattttgga actgtcggct gacatagaac gcggtttgaa
     3421 ttttatcggt gaataaaatt cgtgcaaaga taacaaatgc cgccgtaagc ccgatcggtt
     3481 gtcccctact ctgtctgttt gttcataatt tttgatggag cgcggttccc ggcttcgctg
     3541 tccgccgccg gcggaaccgg tgctcgctca tacgcggtaa tccgctcgcc ccgggctaac
     3601 cggagaaaaa aaatca
//
LOCUS       DASTSF010000299         2168 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_38535,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000299 DASTSF010000000
VERSION     DASTSF010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2168
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38535"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..700)
                     /locus_tag="VFU15_08675"
     CDS             complement(<1..700)
                     /locus_tag="VFU15_08675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717896.1"
                     /translation="MIKRLPLCMLLLAGMVSGRCSGQFSMHWYATQKQNNEKYFLSVG
                     YGVGTAYWYSQMSRTSIYDKQGAQVESGTFKFRANNQTTFYDLNVLCPVQNFRLGLGM
                     DFEKFYLTKLELSGSVFSNKVILYDETFRFDKIFAQVEFPFWPEARSKYSLSANARFG
                     FFSFNNVDRINLFGDDALANSMFTTISPVADMQIYPGIFIFVQPSVEYKYFKNPSVDP
                     GGVVVHNIFTYSAMI"
     gene            complement(709..1719)
                     /gene="bioB"
                     /locus_tag="VFU15_08680"
     CDS             complement(709..1719)
                     /gene="bioB"
                     /locus_tag="VFU15_08680"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013453234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HEU4717897.1"
                     /translation="MSNIRHNWTSEELLELYETPLLELVYRAASVHRQFHNPSEVQVS
                     SLVSIKTGGCSEDCGYCPQSAKYNTNVKVHKILPVEEVVENARNAKSAGVSRMCLGAA
                     WREVRDNRDFDKVLDMVKAINALDMEVCCTLGMLTEEQAQKLSDAGVYAYNHNLDTSS
                     EFYGEIISTRTYEDRLNTLKNVRKAGMTICSGGIIGMGESVNDRLGMLKTLSRLDPHP
                     ESVPINALVPVEGTPLEDQPPVPVWDMVRMVAVARIVMPKSVVRLSAGRLSMSPEGQA
                     LCFMAGANSIFAGDKLLTTPNPAFNEDIELFSALGIKPRAPFAYGKPGTATQEAAALT
                     TQ"
     gene            1846..>2168
                     /locus_tag="VFU15_08685"
     CDS             1846..>2168
                     /locus_tag="VFU15_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002997555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEU4717898.1"
                     /translation="MRIDIITVLPELLKSPFGHSILKRAQDKGLAEVNVVNLRDYSTD
                     KHKSVDDYAFGGGAGMVMKIEPIAACIEDLRSKRSYDEVIYMSPDGEMLDQRIANRLS
                     TVKNI"
BASE COUNT          516 a          562 c          607 g          483 t
ORIGIN      
        1 cgatcatcgc gctgtaggtg aaaatattgt gaacgaccac gccgcccgga tcgaccgaag
       61 gatttttgaa atacttgtat tccaccgaag gctgcacaaa aatgaagatg ccgggataga
      121 tctgcatatc ggccaccggg ctgatcgtgg tgaacatgct gttggcgagt gcatcatcgc
      181 cgaacaggtt gatgcggtcg acattgttga agctgaaaaa accgaagcgc gcattggcgc
      241 tcagcgaata ttttgagcga gcttccggcc agaacgggaa ttccacctgc gcgaatattt
      301 tatcgaagcg gaacgtctca tcgtaaagga tgactttgtt actgaaaacg ctaccggaga
      361 gctcgagctt cgtgaggtag aatttttcaa agtccatccc gagaccgagg cggaaattct
      421 gcaccgggca aagaacgttg aggtcgtaaa atgtagtctg gttgttggcg cggaacttga
      481 atgtccccga ttccacctgc gctccctgct tatcgtagat cgatgtgcgc gacatctgcg
      541 aataccagta cgccgtccct actccataac ccaccgaaag aaaatatttc tcgttgttct
      601 gtttttgcgt ggcgtaccag tgcatggaaa actggccgga gcaacgtccg gagaccattc
      661 cggcgagaag aagcatgcaa agcggtagcc tttttatcat caggggggtt attgagttgt
      721 aagcgctgca gcttcctgcg tggcggttcc gggcttcccg tatgcgaaag gcgcacgggg
      781 cttgatgccg agcgcgctga acaattcaat gtcttcgttg aacgcagggt tcggtgtcgt
      841 cagcagcttg tcgccggcga agatggaatt cgccccggcc ataaagcaga gcgcctgccc
      901 ttcggggctc atggaaagac gtcccgcaga taaacgcacg acagacttcg gcatcacgat
      961 gcgggccacg gctaccatgc gcaccatatc ccagacggga acaggaggct gatcttccag
     1021 cggtgtccct tcaacaggca ccagcgcatt gatgggaacc gattccgggt gcggatcgag
     1081 acggctcagc gttttcagca tgccgagacg atcgttcacc gattcgccca tcccgataat
     1141 tccaccggaa cagatcgtca ttccggcttt gcgcacgttt ttcagcgtat tcaaacggtc
     1201 ctcgtaagtg cgcgtggaaa tgatctcgcc gtaaaattcc gacgatgtat cgaggttgtg
     1261 gttgtaagca taaacaccgg cgtcggaaag tttctgcgcc tgttcttccg tgagcatgcc
     1321 gagcgtgcag cagacttcca tgtccagcgc attgatggct ttcaccatgt cgagcacctt
     1381 atcgaagtcg cggttatcgc gcacttcccg ccacgcagcg cccaggcaca tgcgggaaac
     1441 gccggctgat ttggcgttcc gtgcattttc caccacttcc tctacgggaa ggatcttgtg
     1501 gaccttcaca ttggtattgt atttcgccga ctgcgggcaa tagccgcaat cttcggaaca
     1561 gccgcctgtt ttgatggaaa cgagggagct gacctgcact tcggacggat tatgaaactg
     1621 gcggtgaacg gacgcggcgc ggtataccaa ttccagcagc ggcgtttcgt acagctcaag
     1681 aagttcttcg gatgtccagt tatgccggat gttgctcata atgagaatta tcaaagttaa
     1741 caaaatgggc ttcccttcga cgggagtgtg taaaaatagg cataattggc agggctgcat
     1801 tgaaccggga tgagaaagcg gacttcaaac cgtaaatttg ccgccatgcg catcgacatc
     1861 atcaccgttt tacccgagct gctgaaaagt cctttcggtc attccatcct gaaacgcgca
     1921 caggacaaag ggctggcgga agtgaacgtg gtgaacctgc gggactattc cacggacaag
     1981 cacaaatcgg tggatgatta cgctttcgga ggcggcgcgg gaatggtgat gaagatcgaa
     2041 ccgatcgcgg cctgcatcga agaccttcgg tcgaaacgca gctatgacga agtcatctac
     2101 atgtcgccgg acggagaaat gctcgaccag cgcatcgcga accggttgtc gacggtgaag
     2161 aacatcat
//
LOCUS       DASTSF010000300         3831 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_898590,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000300 DASTSF010000000
VERSION     DASTSF010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3831
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_898590"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1499
                     /locus_tag="VFU15_08690"
     CDS             <1..1499
                     /locus_tag="VFU15_08690"
                     /inference="COORDINATES: protein motif:HMM:NF019284.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HEU4717899.1"
                     /translation="EYDAYGKEGKWLHLDDKGRLLREENYSKGKLDGDFVLYYPETQV
                     VKMTTHYAAGIQSGAHTSFYADAKKDTVSFYNAGGMRDGKMTVYLADGTVCYEATFVN
                     GIPTGTSKRWYDNHQLMEVDNFDSNGQPDDTCLVYYENGQTKSVSVYSHGTREGKWTA
                     WYANGKKQSEANYRKGRKTGTFSDWNSSGILVSQASYKNDTLNGSCKTFYGSGTAKTD
                     YRYTMGIRDGKCTRYDTSGNVTFTRTYAKGKPLNYSPPRKIPGASRCTPDPASPFSLC
                     DSLQEAFHNEAVFIAMRTMWTDSAWKDSVVVRESMIRSIERSLVAVWNFLPPGSFGFT
                     WEYGLPRENGLHELSITYTDKDSFNQAVYNNWKNGKGETGISGIDRILKKYSLTLVPN
                     TFYAYQNEFSVSYTTPLVLNFRGLTAELQKADGHFAAYTSWGTAGDGDHVYQQRKGNI
                     TLLTFSHGYGDCPSGCMGRENFVFIVYDDGEVETGDRIRAMSPYLRRN"
     gene            1569..2336
                     /locus_tag="VFU15_08695"
     CDS             1569..2336
                     /locus_tag="VFU15_08695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717900.1"
                     /translation="MIRLLSIASFVFLFSTIRAGDENGTGCAPRKGSCDVCDSLHALY
                     HLEAICFALDEVSDSTSPWHDSLFVPQALIDKFEHALPVLYNNLPADSVPRVQPVYLS
                     SFYRFVFIVDAHKYKKWVSDTVASHDPAIDALLEKAHLHRQGSETYTTKNSSQMEYTG
                     DRLINPGALQRKFIEAGRENILVTTNAGSLYMPWHSGGYTRMRTRGNDVVADIVLPTG
                     PGLPPELPFPVGKWTYDIDPAACTLKLVSVENPYRGK"
     gene            2412..3356
                     /locus_tag="VFU15_08700"
     CDS             2412..3356
                     /locus_tag="VFU15_08700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717901.1"
                     /translation="MNRFLLPVFFFACVFCISFTPQYEPEIFPDYTSGRTNPEDVLHG
                     NVKSVVTREFSASGTAANIIKGEPQDIFRVSGRTDYNGNNLPLLSAPADPKEAKCTRT
                     VFTYDAKNRPARRVMYSGCRKGETSDYHYNASGRLEKITCFEGDDTLKPVYTISFSYT
                     SSGQLAEEKKNDVVTASYTYGQKNNVVSKSIFKNGKLSGSETYAYNDSGQVTTRTAYD
                     SWKTVTGTWTYTRDAKGDMIRDTFVAGGAKNDTTVNTRTFTYNSSGQLVEEEDRSTAD
                     KSVFTMKFTYDAQGNCTRKVFFSDGKPQTIIERKIEYN"
BASE COUNT          995 a         1032 c          987 g          817 t
ORIGIN      
        1 gagaatatga cgcttacgga aaagaaggaa aatggctgca cctcgatgac aagggacgtc
       61 tcctgcgcga agaaaattat tcgaagggaa agctggacgg cgatttcgtg ctctattacc
      121 cggaaacgca ggtggtgaaa atgaccacgc attatgcagc cggaattcag tccggtgcgc
      181 acacaagttt ttatgcggac gcgaaaaaag acaccgtttc tttttacaac gcaggcggga
      241 tgcgcgacgg gaagatgacc gtttacctcg ccgacggcac ggtctgctac gaagcgacat
      301 tcgtgaacgg cattcccacc ggcacatcga aacggtggta cgacaatcac cagctcatgg
      361 aggtcgacaa cttcgacagc aacgggcaac ccgatgacac gtgccttgtt tattatgaga
      421 acgggcagac gaaatcagtg tccgtttaca gtcacggaac acgggaaggc aaatggacgg
      481 cgtggtacgc aaacggtaaa aaacagtccg aggcgaatta cagaaaaggc cggaagaccg
      541 gaactttctc tgactggaac agcagcggca tcctcgtttc gcaggccagt tacaaaaacg
      601 atacgctgaa cggaagctgc aaaacatttt acggcagcgg aacagcgaaa acagattacc
      661 ggtacaccat gggcatacgc gacgggaaat gcacgcgtta cgacacgagc gggaacgtca
      721 cgttcacgcg cacgtatgca aagggaaagc cgttgaatta ttccccgccg cgcaaaattc
      781 ccggcgcttc acgttgcaca cccgatccgg cttcgccctt ttcactctgc gattcattgc
      841 aggaagcgtt tcacaatgaa gccgtattta tcgcgatgcg aacgatgtgg accgacagcg
      901 cgtggaaaga cagcgtcgtc gtacgtgaaa gcatgatccg gtcaatagag cgcagccttg
      961 tcgcagtatg gaattttctc cctcccggct ctttcggttt tacatgggaa tacggtcttc
     1021 cccgggaaaa cggcctccac gagctgtcca ttacctacac ggacaaagac agcttcaacc
     1081 aggcggttta caacaactgg aaaaacggga agggagaaac agggatcagc gggattgacc
     1141 gcatcctgaa aaaatattcg ctcaccctcg tgccgaatac attttacgcc taccagaatg
     1201 aattctccgt gagctacacc actccgctcg tgctcaactt caggggactt accgcggagc
     1261 tgcagaaagc cgatggacat ttcgccgctt acacctcgtg gggaactgcc ggcgacgggg
     1321 atcatgttta ccagcagcgg aaaggcaaca tcacgctgct tacgttctca cacggctatg
     1381 gcgattgtcc ttccggatgc atgggacgcg agaatttcgt gttcatcgtt tacgacgacg
     1441 gtgaagtgga aaccggtgac aggatacggg ccatgagccc gtatttacga cgtaactgac
     1501 agggagcggg agtttcagtt ccgggtaaag tcagctatct ttcgcgctta ctttcaactc
     1561 tgccaataat gatccgcctg ctgtcaatcg catccttcgt gtttctcttc agtacgattc
     1621 gtgccggcga tgaaaacgga acaggctgtg ctccgcgcaa aggttcctgc gacgtgtgcg
     1681 attcgctgca cgcactttac cacctcgagg cgatctgctt tgcgctggat gaggtgagcg
     1741 acagcacttc accctggcac gacagccttt tcgtaccgca ggcgctcatc gataaattcg
     1801 agcacgcgct tcccgtgctt tacaataatc ttcccgccga ttcggtcccg cgcgtacaac
     1861 cggtttacct ttcttctttt taccggttcg tcttcatcgt cgatgcgcac aaatacaaaa
     1921 agtgggtatc ggatacggtc gcttcgcacg atccggcgat cgacgcattg ctggaaaaag
     1981 cgcacctgca ccggcaagga tcggaaacct acacgacgaa gaacagctcg cagatggaat
     2041 acaccggcga caggctgatc aaccccggcg cgctgcagcg gaaattcatc gaagcaggcc
     2101 gcgaaaacat tctcgtcacc accaatgccg gctctcttta catgccgtgg cacagcggcg
     2161 ggtacacgcg aatgcgcacg aggggaaacg atgtcgtcgc cgatattgtc ctgccaaccg
     2221 gtccaggcct gccgcccgaa ttgccgtttc ccgtgggaaa atggacgtat gacatcgatc
     2281 ctgccgcctg cacgctgaag cttgtttccg tggaaaatcc ttacagggga aaatgatctt
     2341 tgcgcgccta ccccttttat gcgataaaga aaaacaggtg gaatgttatt tttactgcaa
     2401 ataatcctca catgaaccgt tttcttcttc ctgtcttttt cttcgcgtgc gttttctgca
     2461 tttccttcac accccagtac gaaccggaga tttttcccga ttacacgtcc ggccgcacca
     2521 acccggaaga tgtgctgcat ggtaatgtga agtcggttgt cacccgggaa ttttccgcct
     2581 ccggcaccgc agcgaatatc atcaaaggag agccgcagga tattttcagg gtcagcgggc
     2641 ggaccgacta caacgggaac aaccttcccc tgcttagcgc tcccgcagat ccgaaagaag
     2701 cgaaatgcac aaggaccgtt ttcacttacg acgcgaaaaa ccgtcccgca cgcagggtta
     2761 tgtacagcgg ctgcaggaaa ggcgaaacaa gcgattatca ttacaacgct tccggccggc
     2821 tggaaaagat cacgtgcttc gaaggagatg atacgctcaa gcccgtgtac acgatctctt
     2881 tcagctatac ttcttccggc cagctggcgg aagaaaagaa gaacgatgtg gtcaccgcca
     2941 gttatactta cggacaaaaa aacaacgttg tgtcgaaaag cattttcaaa aacgggaagc
     3001 tttccggcag cgaaacatac gcgtacaacg attcgggaca ggtgacaacg agaaccgcgt
     3061 acgattcctg gaagaccgtg acaggaacgt ggacctacac gcgcgatgca aaaggcgaca
     3121 tgatacgcga tacgttcgtc gcaggcggag cgaagaacga caccaccgtg aatacgcgca
     3181 cgttcacgta caattcttcc gggcagctgg tggaagaaga agaccgttcg acggcagaca
     3241 aatccgtttt cacgatgaaa ttcacttatg atgcgcaggg caactgcacc cggaaagttt
     3301 ttttcagcga cggaaaaccg caaaccatca tcgagcggaa gatcgaatac aactgaaggg
     3361 cgcgcagcat ctccggttga attttcattg gtaaacttcg ctgcgaattg ccggactgtg
     3421 agctgcgtgg gcggataaac ttttccgccg gaatgaaatg caggcggata tcttcaataa
     3481 tatccgcaca acgtaaaaag ccaaatcagc gccccggcga ctgttgtgaa ttgctttcag
     3541 attgtatctt cgccgaagtc acacacaacg tcaacttctc gacccaacaa atgcaagcgg
     3601 ttgtgaattg ctttcagatt gtatcttcgc cgaagtcaca cacaaccgta cgcttcgccc
     3661 gcttcctgtg gaatgtgttg tgaattgctt tcagattgta tcttcgccga agtcacacac
     3721 aacctttctt cgccggtgga gttgcccgct gaagttgtga attgctttca gattgtatct
     3781 tcgccgaagt cacacacaac cgggcgagga ttatgaagct gcaatcaagt g
//
LOCUS       DASTSF010000301        11108 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_182256,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000301 DASTSF010000000
VERSION     DASTSF010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 11108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11108
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182256"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..6855)
                     /locus_tag="VFU15_08705"
     CDS             complement(<1..6855)
                     /locus_tag="VFU15_08705"
                     /inference="COORDINATES: protein motif:HMM:NF039534.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717902.1"
                     /translation="MAAGLNAQVSSYIFSQSAGTYTPITGGTVLGTPTNDDNIFAGNP
                     IGFSFCFNGAQYTQFGVNANGWIYMGPSTGTSSYTSISSGSTNNVISAFNFDLQGEPT
                     TGELRYQTIGSAPNRTLVVQFSNYDAYSSSTNTDTYNFQIRLSESTGQIDIVYGNFVC
                     NSAIRTAQVGLRGASAADYNNLSVANGINTWATPAPGASNAATCEINNTPLVPSSGLT
                     YTFSEPAAPVSPINLVFSGVTTTGMTLTWDDNSTNESTFFVYQSIDNINFTLVNTQAS
                     TSTATTGTQYNYTASGLYTNQIYYWRVLAGNANCGGTPLTGNQTTLSGTMCGTYTIGP
                     TGSYPSLTAAIAAVQTNGVSCPLVFEFQAAYVSTVETFPIVVPFLGCGPTTSITARPE
                     LGATNLSITSNATQTIDFNNATYFSFDGRPGGVGSVSQLTIDNSSTTGNAVRFINDAQ
                     YCGLNYMKVRGVTTSATTGIVAFGNALTSGNSNDFITNSDLTSGATSAAVLVYGSNAT
                     ANTTNANENLSNNVFYDWFGAAAADAAINVTGNSAGWSISNNSFYQTASRTYTSAVTH
                     FAININTTTGTGTGNFVVSGNYIGGTAANCGGSAWTGTGAVAHRFIAVNVTTGSLGTN
                     SVQGNTITNFNFTTTSGATTTNGIWCAINLTGTSANMNVGNVAPNMIGSTTTNGQIVT
                     STSTTGGMTVAINSSASGSINISGNMIGGITANSSSGTTSSSIYGILNSSGIPVISNN
                     TIGSPSQANSLINAASTSATGGHITAISNSSGTGAVAINNNIIANLTNQYAGTSTTGQ
                     TRGIVNTSGVATISGNSITTLFNFAPQTGTGTSASVIGIVQSSTSTGEQSVTGNVIAN
                     LANGAATANVTVTGIIMSGPTTIFTNKVNRNNIQSLVSISSGTAIINGIVISGGIIRV
                     YNNMVTLGLDPIGNAVVSPYEINGIYNTTTNRTSILFNSVLITGSNVASGTGNTYGYR
                     CSVTTAAASDSIYNNVFANTRSNSTTGGIHYSISLNAASNTVLNGNVYFGNGTGYQTG
                     HVATTDYASINTWMTAVPGQDLNSYQVNPNFQSNTNLHINNASPTALESRAIPVGNIV
                     NDIDLNQRPGPVGSVNGGGTAPDIGADEFDGFPLSIDMSAILLVRPMTSGCHGSADSV
                     VVRVKNISSQTIDFATNPCTVNAYTQGVNPQVFPPVSLTGTLAGLATMDVVVSTTYNM
                     SATGTHTFRAYTSTTGDLVNSNDSMTAVNILISGGTAVASRPVICMGDSSMLSVSGYT
                     NGGTIQWQSSPDNMTWSNVPGATTTSLNTGALSATTYYRAMICGTDSSVADTVTVNIV
                     SAPTVNGDTRCGAGVVNLTATGSGTIFWYTQPTGGSSVATGTTYSPTVSTTTTFYAEN
                     FSGTPPSSQLTTLAAGNGSTGNMFSITALNTVTITNFDGHMPAGTANWEVWYRPNDYL
                     LSAPASLNSNAGWTMLGAANNVVSNGTGVATPLPIFFNVTIPAGQTYSFYVTTTASTV
                     NYTNGTTTGAVYAPGSNADFQFREGYGGSYFALANNPRIFNGIIHYYSGCGSGRVPVV
                     ATVNPAPAISAMAGSSTLCYGDSTQLVVSSSNTGYAYTWSPASGLSATTGDSVMASPS
                     SPGYFTYYVDALDAGSGCAIRDSVLIHKGAQIIVTANVNSQTADTVCAGTSVNLDGTL
                     PPSTIQVTNGNVLNTSTTYPSPYGNWFGGSRHQMLILASELTAAGMSAGYINGLQFQV
                     TNTNASDPLANFEIDMAQTNVTSITTFQTLPFVTVYTNASYAPVVGFNTHTFSTPFYW
                     DGVSNIIVQTCFVNLTSPTTTSYTYNCTMRQTATAFSSTVLNYADNTPTICTDASTTS
                     YNQRPNIAFMMSYPYAYSWTPSVNVASPTSLSTTANPMVNTTYVLTVTDTTTGCMAMD
                     SVMVRVNPNPAPNFGPDTAICSNTTLVLDGTAGNYSYLWQDNSTSQTYSVNAFGTYGV
                     VVTDTITGCAGGDTILVGINSAPVFTLGSDATVCSGTQVTFSGPGGQYSYGWNTSDTT
                     VSITTGTAGNYILVVTDTVNNCFSSDTVALAVNPAPAVFLGNDTSVCDGNGPLVLNAP
                     SGNYSYSWSDASTNQTLPVTVTGNYYVIVTDNVTSCFSGDTILVNVNTSPAFTLGNDT
                     TFCSGNGPIMLSGPAGPYNYMWSDASTGMTLSENTTGNYYLTVTDSVNGCMTSDTIAL
                     TVPMSPPAVLSDTMVCGSSVTLSGPSGSYNYSWSPTGDMTQTETINAPGGMYYLTTTD
                     PSSGCATTDSSMVAINTPPTVTFAFGQASICTTDAPL"
     gene            complement(7303..7842)
                     /locus_tag="VFU15_08710"
     CDS             complement(7303..7842)
                     /locus_tag="VFU15_08710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717903.1"
                     /translation="MNNDDDNKEKNISPFYHHTDNFSSAYFGKIPEHVNPSASESGEN
                     ALMASVISLLAGPQKNEHRPELLALLRSNKADGLLLKMLGRKDYLKYRKELLAACWES
                     GLDFSKHLSFFAGMTLNADYESCIEITTIITECMQGPFEKETTEAAISEMKRAGANDS
                     VKMQLLETAITFLNIRSVT"
     gene            7938..9188
                     /locus_tag="VFU15_08715"
     CDS             7938..9188
                     /locus_tag="VFU15_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015030896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEU4717904.1"
                     /translation="MEAEIITIGDELLIGQVTDTNSGWMGQQLNRNGVNVRYRSAVSD
                     RAEDISSVLDIALKRSTLVLITGGLGPTKDDITKKTLCDYFGMGWRTDQLVLADVRGF
                     FKRFGREVTEVNLQQANVPDGCVALRNKLGTAPGMWFERDGRIIVSLPGVPHEMKGLM
                     TDEVLPRIMQKFALPGIVHKTILTQGIGESWLAEKIAAWEDSLAAEHIRLAYLPSPGI
                     VRLRLSIYEGKKEDAEKCIARKMEEVLPLIHDYVFGFDDDTLENVVGSLLRERKKTIS
                     TAESCTGGNIAHMITSVPGSSDYYIGSVIAYADRIKENQLDVLPETLAKDGAVSEYVV
                     KEMAVNARKKLVTDYAIAVSGIAGPGGATEAKPLGTTWIAVSGPEGTFAKKFIFGDSR
                     ERNILRASVAGLAFLRKLILGELK"
     gene            9198..>11108
                     /gene="ligA"
                     /locus_tag="VFU15_08720"
     CDS             9198..>11108
                     /gene="ligA"
                     /locus_tag="VFU15_08720"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017843078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEU4717905.1"
                     /translation="MTKEQAHKRIEELRKELTEHNYRYYVLAEPVISDYDFDQMLDEL
                     IALEKQYPEFYSPDSPTVRVGGEITKEFVTVKHRYPMLSLGNTYSEEELRDFDERVRK
                     GISSDEVEYVCELKYDGVAIGLRYEKGKLVQAVTRGDGEKGDDITANERTIRSIPLSL
                     KKSSWPDDFEIRGEVFMSRKVFNAINREKEDIGEAPLANPRNSTAGTLKMQDSAIVAS
                     RKLDSFMYFVLGEDLPFRTHYESLEAAREWGFKVASYSKKVKGLDAVMKFIAHWDKER
                     FNLDFDIDGVVIKVNDLDQQRSLGFTAKSPRWAIAYKFKAERVATKLLSISYQVGRTG
                     AITPVANLQPVLLAGTTVKRASLYNEDSISKLDVRAGDTVYVEKGGEIIPKIVGVDLS
                     KREAHSHPNKYITHCPECGALLVRREGEALHYCPNENGCPPQLKGKIIHFVSRRAMNI
                     ESLGEETIGQLFDAKLIHGIGDLYSLKNKKEQLLALDRMAEKSVNNLLEGIDESKNVP
                     FERVLFALGIRHVGETTAKKIATHFRNIDAVMHASREKLVDVPEVGEILADSIHDYFS
                     NEKNRELITLLKRAGIRFELSEEQLAGRSEKLAGKTFVVSGVFSIPRDDLKALIEKNG
                     GKNTGSVSGKTSY"
BASE COUNT         2585 a         2663 c         3290 g         2570 t
ORIGIN      
        1 aagcggcgcg tccgtggtgc agatcgaagc ctgtccgaaa gcaaacgtta ccgtcggcgg
       61 agtgttgatg gctaccatcg aggaatcggt ggtcgcacat cccgaagacg ggtctgtcgt
      121 ggtaaggtaa tacatgccgc cgggagcgtt gatggtttcg gtttgtgtca tatcgccggt
      181 cgggctccag ctgtaattgt aggaaccgga aggaccggaa agtgtaacgg aagaaccgca
      241 gaccatcgtg tcagaaagta cggcaggcgg tgacatcggt acagtaagtg cgatcgtgtc
      301 gctcgtcata catccgttga cagaatccgt aactgtgagg taatagttcc cggtggtgtt
      361 ttcactgagc gtcatacccg tggaagcatc gctccacatg taattgtacg gaccggccgg
      421 accactaagc atgatcggac cgtttccgct gcagaacgtc gtatcgttgc ccagcgtgaa
      481 cgcaggcgac gtattcacgt tcaccaggat cgtatcgccg ctgaagcagg aagtaacgtt
      541 atccgtgacg atcacatagt agttgcccgt tacggtgacc gggagcgtct ggttggtgga
      601 agcgtcgctc caggaatagc tgtaattacc tgaaggagca ttcagcacca gcggtccgtt
      661 tccgtcgcac acagaagtgt cgtttccgag gaagacggcc ggagccgggt tgacggcaag
      721 cgcaacggta tcgctgctga agcagttgtt gaccgtgtcc gttaccacaa gaatgtaatt
      781 gcctgctgtt cctgtcgtga tggaaacggt agtatccgaa gtgttccatc cgtaggaata
      841 ctgtccgccc ggaccactga acgtaacctg cgtaccggag caaacagttg cgtcagaacc
      901 gagcgtgaat acaggtgcgg aatttattcc gacgaggatc gtgtctccgc ctgcgcatcc
      961 cgtgatagta tccgttacaa caacaccgta cgttccgaac gcgttgacag agtatgtctg
     1021 gctggtggaa ttatcctgcc agaggtagct atagtttcct gccgttccgt caagcaccag
     1081 cgtggtgttg gagcagatcg ctgtgtcggg tccgaagttc ggcgccgggt tcggatttac
     1141 gcgtaccata acagaatcca ttgccatgca gccggtggtc gtatcggtaa ccgtaagaac
     1201 gtacgtggta ttcaccatcg ggttcgcggt cgtagaaagc gacgtcgggg atgcaacgtt
     1261 tacagaaggc gtccaggagt aagcgtaagg atatgacatc atgaacgcga tgttcggacg
     1321 ctggttgtaa gaagtcgtgg aagcgtcagt gcagatcgtc ggtgtattgt cggcgtaatt
     1381 cagaacggta gaactgaagg ccgttgctgt ctggcgcatc gtacagttgt aggtgtagga
     1441 cgtcgtcgtc gggctggtca ggttcacgaa gcatgtctgc acgatgatgt tggaaactcc
     1501 gtcccagtag aacggtgtgc tgaacgtatg cgtgttgaag ccgaccactg gcgcgtaaga
     1561 cgcgttagtg tatacggtta cgaagggcag cgtctggaat gtagtgatag atgttacgtt
     1621 cgtctgcgcc atatcgatct cgaaattggc gagtgggtcg gacgcattcg tgttcgtcac
     1681 ctggaactgc agaccattga tataacctgc gctcattccg gcagccgtaa gttcgctggc
     1741 caggatcagc atctggtgcc gcgaaccgcc gaaccagttg ccataaggag aaggatacgt
     1801 ggtagaggtg ttgagaacgt taccgttcgt cacctggatt gttgaaggag gaagcgttcc
     1861 gtcaaggttc accgaagttc ctgcacacac ggtatcagcg gtctggctat tcacatttgc
     1921 cgtaacgatg atctgcgcgc ctttgtggat cagcacggaa tcgcggatcg cgcaacctga
     1981 tcctgcatcg agtgcatcaa cataataggt gaaataaccc ggtgatgaag gagaagccat
     2041 taccgaatca cctgttgttg ctgaaagtcc tgatgcagga gaccatgtat acgcataacc
     2101 cgtatttgaa ctggaaacaa ccagctgcgt cgaatcgccg tagcagagtg ttgaagagcc
     2161 tgccatcgcg gaaatcgcgg gagccgggtt gaccgttgca accacgggaa cgcgaccgct
     2221 tccgcacccg gaataatagt ggatgatgcc gttgaaaata cgcgggttgt tcgcaagagc
     2281 gaagtagctt ccgccatatc cttcacggaa ctggaaatct gcatttgaac cgggggcata
     2341 aacagcgccg gttgtcgttc cgttcgtata attcacagta gaagccgtcg tcgtaacata
     2401 gaacgaatac gtctggcctg cggggatcgt tacgttgaag aagatcggaa gaggcgtagc
     2461 aacaccggtt ccattcgata cgacgttgtt tgccgcgccg agcatcgtcc atcctgcgtt
     2521 actgttcagg gaagcgggag cagaaaggag gtaatcgttc gggcgatacc agacttccca
     2581 gttggcagtg cctgcaggca tgtgtccgtc gaaattggtg atggtgaccg tattcagcgc
     2641 ggtaatggag aacatgttgc ccgtcgatcc gtttcctgct gcaagtgtag tcagctggct
     2701 gctcggcggc gttccgctga agttttctgc gtagaaagtt gtggtcgtgc ttaccgtcgg
     2761 tgaataggta gtgcctgttg caacagagct tccgccggtc ggctgcgtat accagaaaat
     2821 tgttcccgat ccggttgccg tcaggttgac tacacccgcg ccgcaacgcg tatcgccatt
     2881 gaccgttggt gcactgacaa tatttacagt taccgtatcc gctacagacg aatccgttcc
     2941 gcagatcatg gcacggtaat acgtggttgc tgaaagcgca cccgtattca acgaagttgt
     3001 agtcgcgccc ggaacgtttg accaggtcat gttatccgga gatgactgcc actggatcgt
     3061 tccgccgttg gtgtaaccgc ttacggaaag catgctggag tcgcccatgc agatcaccgg
     3121 gcgagaggca acagcagtac ctccggagat caggatgttc accgctgtca tggagtcgtt
     3181 gctgttcacg aggtcgcccg ttgtggacgt atacgcacgg aacgtgtgcg ttccggttgc
     3241 gctcatgttg tacgtcgtgc tgacaaccac gtccatcgtg gcgagtccgg caagcgttcc
     3301 cgtcagggaa accggcggaa atacctgcgg gttgacgccc tgtgtatagg catttaccgt
     3361 gcacgggttc gttgcaaagt cgatggtttg cgagctgatg ttttttacac gcacgacaac
     3421 ggaatcagcg ctgccatgac atccgctcgt catcgggcgg acgaggagaa ttgcgctcat
     3481 gtcgatcgag agcggaaagc cgtcaaattc atcggcgccg atatcgggag ctgtgccgcc
     3541 gccgtttaca gagccgaccg gacccggacg ctgattgaga tcgatgtcat ttacaatatt
     3601 tccaaccgga attgcacgcg attcaagcgc ggtaggggaa gcgttgttga tgtgcaggtt
     3661 ggtgttcgac tggaaattcg ggttgacctg gtaagagtta agatcttgtc ccggtacagc
     3721 cgtcatccag gtgttgatgg acgcgtaatc cgtcgtcgca acatgccccg tctggtagcc
     3781 tgttccgtta ccgaagtaaa cgtttccgtt cagcacggtg ttcgaagcgg cgttcagcga
     3841 aatggaatag tgaattccac cggtcgttga attggaacgc gtgttcgcaa acacgttgtt
     3901 gtagatagaa tccgaagctg ctgcagttgt taccgaacaa cgataaccgt atgtatttcc
     3961 ggtcccgctg gcgacgttcg atcctgtaat gagaaccgag ttgaaaagaa tgctggtcct
     4021 gttggtcgtt gtattgtaaa tgccgttgat ctcgtacgga gaaacaaccg cgttaccgat
     4081 ggggtcaagt ccaagagtaa ccatgttgtt atacacccgg atgatcccgc cggagataac
     4141 gataccgttg atgatcgccg tgccgctgct gatggatacg agcgactgga tattgttgcg
     4201 gttcactttg ttggtaaaga tcgttgtcgg gcctgacatg atgataccgg tcaccgtgac
     4261 gttggcggta gcagcgccgt tcgctaagtt ggcgatcaca ttgccggtaa ctgattgctc
     4321 cccggtcgat gtagatgact gaacaatgcc gatcacggaa gcggaggttc ctgtgccggt
     4381 ttgcggagca aagttgaaca atgtcgtgat cgagttgccg gaaatagttg cgacgccgga
     4441 ggtgttcaca attccacgcg tctggccggt tgtggaagtt ccggcgtact ggttggtcag
     4501 gttggcaatg atgttgttgt tgatagcgac cgcgcccgtt ccggagctat tgctgatcgc
     4561 ggtaatgtga ccgccggtag cgcttgtgct cgctgcattg atcaggctgt ttgcctgcga
     4621 aggacttccg atcgtgttat tgctgatgac cgggatacct gacgagttca ggattccata
     4681 aatgctggaa gaggtcgtgc cggaagacga gttggctgta ataccgccga tcatgtttcc
     4741 gctgatgttg atgctgccgg atgcagatga gttgatcgcg acggtcattc caccggttgt
     4801 gcttgtactg gtaacgatct gcccattcgt ggtggtgctg ccgatcatgt tcggagctac
     4861 gttacccacg ttcatgttgg cagacgtacc ggtcaggtta attgcacacc agataccgtt
     4921 agttgtggtt gcgccgctcg ttgtcgtgaa attgaaattg gtgatcgtgt ttccctggac
     4981 gctgttggtt ccgagcgagc ccgtggtgac gttcacggca atgaagcgat gcgcaaccgc
     5041 gccggtgccg gtccatgccg atccgccgca gtttgccgcg gttcccccga tgtagttgcc
     5101 ggagacaacg aagttgccgg ttccggttcc tgtagtcgtg ttgatgttga tcgcgaaatg
     5161 agttacagcg ctcgtatacg tgcggcttgc cgtctggtaa aagctgttat tgctgatgga
     5221 ccatcccgct gaatttccgg tcacgttgat ggcagcatca gcggcggcag caccgaacca
     5281 gtcatagaaa acgttattgc tcaggttctc gttggcgtta gtcgtattgg ctgttgcatt
     5341 ggatccgtaa acaagaacag ctgcgctggt tgccccgctg gtaagatcgc tgttggtgat
     5401 gaaatcgttg ctgtttccgc tggtaagcgc attgccgaac gcaacgatac ccgtggtcgc
     5461 ggaagtcgtg acgccgcgca ccttcatgta attaaggccg cagtactggg cgtcattgat
     5521 aaagcgcacc gcattgcccg tggtggagct gttatcgatc gtaagctggg aaacagaccc
     5581 tacaccaccc ggacggccgt cgaagctgaa ataggtggca ttgttgaagt cgattgtttg
     5641 tgtggcgttg ctcgtaatgg aaaggttggt tgcgccgagt tcagggcgtg cagtgattga
     5701 cgttgtggga ccgcatccga ggaagggaac gacgataggg aacgtttcca ctgtgctgac
     5761 ataagccgcc tggaattcaa aaacgagcgg gcagctcacg ccattcgttt gcactgcagc
     5821 gatggctgcc gtcagtgaag gataagaacc tgtgggcccg atcgtgtagg tgccgcacat
     5881 tgttcccgac agcgtggtct ggttgccggt aagcggtgtt ccgccacagt tggcattgcc
     5941 ggctaaaaca cgccagtaat aaatctggtt ggtataaagc ccgctggccg tatagttgta
     6001 ttgtgttccc gtggttgcgg ttgaggtcga ggcctgggtg ttaaccagcg tgaaattgat
     6061 gttgtcgatc gactggtaaa caaaaaatgt agattcattg gtggagttat cgtcccacgt
     6121 aagcgtcatt cctgttgtgg tgacgccgga gaaaacgagg ttgatcgggg aaacgggagc
     6181 tgccggctcg gaaaaggtat atgtaagtcc ggaagacgga accagcggag tattgttgat
     6241 ctcgcaggtg gcagcgttgc tggctcccgg tgccggggtc gcccatgtat tgatgccgtt
     6301 cgcaacggac aaattgttgt aatcggctgc agatgcaccg cggagaccga cctgtgccgt
     6361 acgtatcgca gagttgcaca cgaagttgcc gtaaacaatg tcgatctggc ccgtcgattc
     6421 gctcaggcgg atctggaaat tgtacgtatc cgtgtttgtc gaactgctgt acgcatcata
     6481 atttgagaac tgcaccacga gcgtacggtt cggagccgag ccgatcgtct ggtagcgaag
     6541 ttcaccggtt gtcggttcgc cctgcaggtc aaagttgaat gcagaaatga cgttgttcgt
     6601 tgaacccgag ctgatggacg tataagagct ggtaccggtg gagggaccca tgtatatcca
     6661 tccgttggcg ttgacaccga actgggtgta ttgagcgccg ttgaaacaga acgagaagcc
     6721 gatcgggttg cccgcgaaga tgttatcatc gttggtcgga gttcccagta cggttccgcc
     6781 ggttatcggc gtatatgtgc ctgccgactg gctgaaaatg tagctggaca cctgtgcgtt
     6841 caggccggcg gccataaaca gggcaaagag aaaagacaga aaggctgcat gggaaaacgt
     6901 gcgcttgccc cggttagctt tcggtctgtt ttgcgtatac atttttttca tgtttaggat
     6961 ttatgtttat tgtgtgtggg tgattgcgga ttttaaaggg cgataaaatt agggaagtca
     7021 gctaagagcg ttgtttacaa tcatcgaaaa aagtgattat tgttaataat taataacggc
     7081 tttttattca tttgggatgt tttgtgttat taatcagttg tttgtgtggt ttgggggccc
     7141 aatgttatca acatgattac cggcctgcaa ttgacctttt cggggaaaaa tattccgggc
     7201 caaaactaaa aatcagccgt attgaatgat aagatcattc gttcaatgaa accgatcatt
     7261 ccatgaaatg cagaaaacag gggataagag ggaaaaggag aatcaggtga cgctgcgaat
     7321 attcaggaag gtgatcgccg tttcgagcag ctgcattttt acactatcgt tcgcaccggc
     7381 ccgcttcatt tccgagatgg cggcttcggt cgtttctttt tcgaaaggcc cctgcatgca
     7441 ttcagtgatg atggtggtta tttcaatgca ggattcatag tccgcattca gggtcatccc
     7501 ggcaaagaac gaaaggtgct tcgaaaaatc gagcccgctt tcccagcagg cagccagcag
     7561 ctctttcctg tatttaaggt aatccttcct gccgagcatt ttcagcagca gcccgtccgc
     7621 tttgttcgaa cgcagcagtg caagcagttc cgggcggtgc tcgtttttct gcggacccgc
     7681 cagtagcgat atgaccgaag ccatgagggc gttctcgccg gattccgaag cagaaggatt
     7741 tacatgttcc ggaatttttc cgaaataggc gctgctgaaa ttatcggtgt ggtgatagaa
     7801 cggggagatg ttcttctctt tgttgtcgtc gtcgttgttc atcgcataaa agatgaggca
     7861 aagttacgat aacaacacgt aagaatttaa ttcccggaat gtgtatccgc agggaacgaa
     7921 acttaatttt gcgtgatatg gaagcagaaa taattaccat cggcgacgag ctcctcatcg
     7981 ggcaggtgac ggataccaat tcgggatgga tgggacagca gctcaaccgc aatggcgtca
     8041 acgtacgcta ccgttcggct gtttccgacc gcgcggaaga tatttcctcc gtgctggaca
     8101 tcgcgctgaa gcgaagcacg cttgtcctga tcaccggcgg gctgggtccg acgaaagacg
     8161 acatcacgaa aaaaacattg tgcgattatt tcggaatggg ctggcgcacg gatcagcttg
     8221 ttctcgcgga tgtccgcggt tttttcaaac gtttcggaag ggaagtcacg gaggtcaacc
     8281 tgcagcaggc gaacgtgccc gacggatgcg tcgccctgcg caacaagctg ggaaccgcgc
     8341 ccggaatgtg gttcgagcgg gacggcagaa taatcgtttc tcttcccggc gtgccgcacg
     8401 agatgaaagg actgatgacg gacgaagtgc tgccgaggat catgcagaaa ttcgcgttac
     8461 ccggcattgt tcacaaaacg atattgacac agggcatcgg cgaatcatgg ctggcggaaa
     8521 agatcgcggc gtgggaagat tcgctggcag cggagcatat ccgcctggct taccttcctt
     8581 cgccgggcat tgtgcgcttg cgtctttcca tttacgaggg aaaaaaagag gatgcggaaa
     8641 aatgcattgc gcgcaaaatg gaagaagttc tgccgctgat ccacgattat gttttcgggt
     8701 ttgatgacga tacgctggag aatgtcgtcg gcagcctgct ccgtgaaagg aaaaaaacga
     8761 tttccacagc agagagctgc accggcggaa acattgccca catgatcact tcggttcccg
     8821 ggagttccga ttattacatc ggcagtgtga tcgcgtacgc cgaccgcatc aaggaaaacc
     8881 agctggatgt gttgccggaa accctcgcaa aggatggcgc ggtaagcgaa tacgtggtaa
     8941 aagaaatggc cgtcaacgcc aggaaaaagc tggtaaccga ttatgcgatc gccgtttcgg
     9001 gaattgcagg tccgggcggg gcaaccgaag caaagccatt gggcacgacg tggatcgcgg
     9061 taagcggacc ggaaggcacc ttcgcgaaaa aatttatttt cggcgatagc cgtgagcgca
     9121 atatcctgcg ggcttccgtg gccggacttg ccttcctgcg aaaacttatt ctcggcgagc
     9181 tcaaataacc aatgcacatg acgaaagaac aggcgcacaa gcgcatcgaa gagttgcgga
     9241 aagagctgac ggagcacaac taccgttatt atgttctcgc cgaacccgtg atcagcgatt
     9301 atgatttcga ccagatgctc gacgaactga tcgcgctgga aaagcagtat cccgaattct
     9361 attcccccga ttcaccgacc gttcgcgttg gcggcgagat cacgaaagag ttcgtgaccg
     9421 tgaagcaccg ttatcccatg ctttcgctgg gaaatacgta cagtgaagaa gagctgcggg
     9481 atttcgacga acgtgtgcgc aagggaattt cgtccgacga agtcgaatat gtgtgcgagc
     9541 tgaaatacga cggtgtggcg atcgggctgc gttatgaaaa aggaaagctc gtgcaggcgg
     9601 tgacgcgtgg cgacggcgaa aaaggcgacg acatcacggc caatgaacgt acgatacgaa
     9661 gcatcccgct gtcgctgaaa aaatcgtcct ggccggatga tttcgagata cgcggagaag
     9721 tgttcatgtc gcggaaagtg ttcaacgcga tcaacaggga gaaagaagac atcggcgaag
     9781 cgccccttgc caatccgcga aattccacgg caggaacgct gaagatgcag gattcggcta
     9841 tcgtcgcttc ccggaagctc gacagcttca tgtattttgt cctcggggaa gatcttccgt
     9901 tcagaacgca ttacgaaagc ctggaagccg cccgtgaatg gggattcaaa gtagcctcgt
     9961 attcgaaaaa agtgaaaggg ctggatgcgg tgatgaaatt catcgcgcat tgggacaagg
    10021 aacgattcaa tctcgatttc gatatcgacg gcgtggtgat caaggtgaac gacctcgacc
    10081 agcagcgttc gcttgggttt acggcgaaat cgccgcgctg ggcgatcgcc tataaattca
    10141 aggcagaacg cgtggccacg aagctgcttt cgatcagcta ccaggtggga agaaccggcg
    10201 cgatcacgcc cgtcgcgaac ctgcagcccg ttcttcttgc aggaacgacg gtgaaacgcg
    10261 catcgctgta caatgaagac agcatcagca agctcgacgt tcgtgccggc gacactgttt
    10321 acgtggaaaa aggcggcgag atcatcccga agatcgtcgg tgtggatctt tcgaaacgcg
    10381 aagcgcattc acatcccaac aaatacatca cgcattgccc ggaatgcggc gcgttactcg
    10441 tacgccggga aggtgaagcg cttcattact gcccgaatga gaacgggtgc ccgccccagc
    10501 tgaaagggaa gatcattcat ttcgtatcac gccgcgcgat gaacatcgaa tcgctgggcg
    10561 aagaaacgat cgggcaactc ttcgacgcga agctcattca cggaataggc gatctctatt
    10621 cgctgaaaaa taaaaaagag cagctgcttg cgctcgaccg catggcggaa aaatcggtca
    10681 acaacctgct ggaggggatc gacgaatcga agaacgttcc gttcgagcgg gtgctgttcg
    10741 cgctgggcat acggcacgtg ggcgagacga ccgcaaaaaa aatcgcgaca catttcagga
    10801 acatcgatgc cgtgatgcac gcttcccgcg aaaagctcgt ggatgttccg gaagtgggcg
    10861 agatcctggc ggacagcatc catgattatt tcagcaacga aaagaaccgc gagctgatca
    10921 cgttgctgaa aagagcaggg atccgtttcg agctttccga agagcagctg gccggaagaa
    10981 gcgaaaagct ggccgggaag acgttcgtcg tttcaggtgt tttctccatt ccgcgcgatg
    11041 atctcaaagc gcttatcgag aagaacggcg ggaagaatac gggttccgtc tcgggcaaaa
    11101 cgtcgtac
//
LOCUS       DASTSF010000302         6986 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_940508,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000302 DASTSF010000000
VERSION     DASTSF010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 6986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6986
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_940508"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..623)
                     /locus_tag="VFU15_08725"
     CDS             complement(<1..623)
                     /locus_tag="VFU15_08725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717906.1"
                     /translation="MKPLPIAVFVLCVCSAGAQVSITNSDMVGAGDSVRVSYAASTGT
                     ADYTVFGPNYLWDFSQLAPYAQQVMRYDQPSAFPFTSMATVAHVNLSPDSIPGLGIIP
                     SDFTDYYKGSSSSFRQVGSSFLYPQVINFPIPVIYSSSDHIYNFPLDYPDHDTSDAAW
                     SFNAQSFGLFYIGETIHRESAVDGWGTLITPFGTFQCLRQVSYVTKV"
     gene            795..1265
                     /locus_tag="VFU15_08730"
     CDS             795..1265
                     /locus_tag="VFU15_08730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717907.1"
                     /translation="MLKRLSLLLLFFIPLLSQAQNDSAIDVPAQLAGKNYSERTFFKD
                     GHLTHQVCYNDPGPADEEFCYTLDLDFASFDSISDRHGYDLVKDTAFVRCSFGVISPQ
                     NRDMDPKAVITGNVFVLGKSKDSIAVLEDIRVTDSAGQVYIYHTQRSFERKKEK"
     gene            1396..5001
                     /gene="dnaE"
                     /locus_tag="VFU15_08735"
     CDS             1396..5001
                     /gene="dnaE"
                     /locus_tag="VFU15_08735"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015030872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEU4717908.1"
                     /translation="MQFNHLHVHTQFSLLDGAAGISSLYKKAVADNMRGIAITDHGNM
                     FGVFKFVAEAGKYNTPENPDKIKPIVGCEFYVVENREKKQFTKEDRDVRYHQLFLAKN
                     AEGYKNLVKLCSLGYVEGLYGKYPRIDKELILKYHKGLIATTCCLAAHIPRMILRKGE
                     EEAEKEFKWWLDIFGEDYYIELQRHDIPDQNKVNEVLLKWAVKYNVKVIASNDSHYVN
                     VEDANAHDILLCINTGEKKSTPSAKEFSDDDSSMKGRRFAFWNDQFYFKTTQEMSDLF
                     ADVPQAIDNTNEIVDKIEPLKLKRDILLPHYKIPEGFTDQDEYLKHLTFEGARRKYKE
                     LTPEIEERINFELFTIRTMGFAGYFLIVQDFINAGKNIGVLIGPGRGSAAGSVVAYCT
                     GITNIDPIKYNLLFERFLNPDRKSMPDIDTDFDDEGRQKVIDYVVDKYGRNQVAQIVT
                     YGTMAAKMSIKDVARVLDLPLDQSNAMAKLVPERPGIALGRVLTAPMDGEKSLREKEQ
                     LGGDELDNVRKLRAIYGGNDLQAEVLKEALVLEGSVRGTGIHAAGIIIAPQDLTDIIP
                     VATSKDTNLFITQYDGKVIEDAGVIKMDFLGLKTLTIIRDALKLIRQNHNIDIEIDEI
                     PLDDPKTFSLYQRGETNGTFQFESNGMQKYLKDLKPDKFEDLIAMNALYRPGPLEYIP
                     KFILRKHGREAITYDLPEMEEFLKDTYGITVYQEQVMLLSQKLAGFTKGDADILRKAM
                     GKKDRATLDKMKGKFMEGTKAKNLDAATCEKVWTDWEAFAQYAFNKSHSTCYAFVAYQ
                     TAYLKAHYPAEYMASVLTHNLGNIDKITFFMEESRRMGIPVLGPDVNESEIHFSVNKK
                     GEIRFGMGAIKGVGEGAAQAIIEERKANGPYKNIFDLVRRVNLRAANKKTLENLVYAG
                     GMDSFTDLHRATYFKNENGENSPTFLEKIIRYGQAQQESRNSSQVSLFDMMGDDGGIE
                     MPEPAVPKAEEWGTLEKLKAEKDVIGIYLSGHPLDDFRTEMVSFCRHKLSDLADLPKW
                     KNHEMHLGGMITSVQHKMTKTGKPFGTFVLEDYDSSYEFALFGEDYVRFKQFILTPGL
                     FILMRGKVQERFGNSENLEVKIHSMELLSELLEKKVKQFTIQLPLPSLSDDLIARVQD
                     IIAKYPGNSALRFTVYDQAENIQLELPSRKVRVKPGNELIRELEAVVPEAGWKLN"
     gene            complement(5416..5721)
                     /locus_tag="VFU15_08740"
     CDS             complement(5416..5721)
                     /locus_tag="VFU15_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEU4717909.1"
                     /translation="MRKYSVYILKCADDSYYTGVTNNLRRRLREHVNASNEGSYTSER
                     LPVELVWSENFRDVRVAIRREKQIKRWSRVKKEALIEGRVDDLKMLAKKKFVKKKVV"
     gene            complement(6127..>6986)
                     /locus_tag="VFU15_08745"
     CDS             complement(6127..>6986)
                     /locus_tag="VFU15_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014680180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoglucomutase/phosphomannomutase family
                     protein"
                     /protein_id="HEU4717910.1"
                     /translation="LAYDAMYGAGQNVIRRLFPDTTMLHCEYNPSFDGQAPEPILRNL
                     QEFADVIRTAGDIDCGLATDGDADRIGLFDNEGNFVDSHHIILLLIHYLVKYKGMKGK
                     VCTAFSTTVKIKNMCRHYGLELETVKIGFKYICGIMVTEDVLLGGEESGGIAIKGHIP
                     ERDGIWMGLVIWEFMAKSGKTLKQLIEEVYAITGPFSFERVDLHLDESVKQRVIADCK
                     AGRYKTFGKYKVDHVEDLDGWKFFLNDETWVMIRASGTEPVLRTYAEAATREEAFDIL
                     AKTKETLLG"
BASE COUNT         1758 a         1901 c         1780 g         1547 t
ORIGIN      
        1 tccactttcg ttacatagga aacctgccgc aggcactgga acgtaccgaa aggcgtgatg
       61 agcgttcccc atccgtcaac ggcgctttcc cggtggatgg tttcgccgat gtagaaaaga
      121 ccgaagctct gcgcattgaa cgaccatgca gcatccgagg tgtcgtggtc cgggtaatcc
      181 agcggaaaat tgtaaatgtg atccgatgag ctgtaaatga ccgggatggg gaaattgatg
      241 acctgcgggt aaagaaagct gcttccgacc tgccggaaag aagaggacga gcctttgtag
      301 taatcggtga aatcggaagg aataatgccc agcccgggga tcgagtcggg cgaaaggttc
      361 acgtgagcca ccgtagccat cgacgtgaac ggaaaggccg agggctgatc gtagcgcatc
      421 acctgctgtg cgtacggcgc cagctgggaa aaatcccata agtaattcgg accgaacacc
      481 gtgtagtcgg ccgttcccgt ggaagctgca tagctcactc gcacggaatc gcctgcaccc
      541 accatgtcac tgttcgtgat cgaaacctgg gcaccggcac tgcacacaca caaaacaaaa
      601 acggcgatcg gcaggggttt cataccaaaa ctttcgccta aagtacactt atccttccag
      661 caggcaaatt agagcattac tttaactcct ttcgtgcatc tctttcagtc tatttatcct
      721 ggacgtcgaa aaacatccgg taacttaccg gcattccccg caactatttt ctttgttttc
      781 agtaaaatta ccccgtgctg aaacgtcttt ccctgctgct gctttttttc atcccgcttc
      841 tttcgcaggc acagaacgat tctgcgatcg atgttcccgc gcagttggcc gggaaaaatt
      901 acagcgagcg tacttttttc aaagacggtc atctgaccca ccaggtttgc tacaatgatc
      961 ccggcccggc agatgaagaa ttctgctata cgcttgatct tgattttgcc agcttcgaca
     1021 gcatcagcga ccgtcatggc tatgatcttg tgaaagacac cgctttcgtg cgctgcagtt
     1081 tcggtgtgat cagtccgcag aaccgggaca tggatcccaa agctgtgata acgggaaatg
     1141 tttttgtgct gggtaaaagc aaagattcca tagctgtgct ggaagacata cgtgtaaccg
     1201 attccgccgg gcaggtttat atttaccata cacagcgttc gttcgagcgg aaaaaagaga
     1261 agtaggcatt accttctcaa caaagtccct cttttatcaa caaaactttt tccccgctcc
     1321 cctgctgctt aattttaaag caccggaatc tcctgatttc cgccattcac tttccaactt
     1381 ttcaactcat tcgctttgca attcaaccac cttcacgttc atacgcagtt ttcccttctc
     1441 gacggggcgg caggcatttc ctccctgtat aaaaaagccg tcgccgacaa tatgcgcggt
     1501 attgccatca ccgatcacgg aaatatgttc ggcgtgttca agttcgtcgc cgaagccggc
     1561 aagtacaata cgccggaaaa cccggacaaa atcaaaccga tcgtcgggtg cgaattttac
     1621 gtggtggaga accgcgagaa aaaacaattc acgaaggaag accgcgacgt gcgttatcac
     1681 cagcttttcc ttgcgaaaaa tgcggaaggc tataaaaatc tcgtcaaact ctgctcgctc
     1741 ggttacgtgg aaggacttta cggaaaatac ccgcgcatcg acaaagagct catccttaaa
     1801 taccacaaag gactgatcgc gacgacgtgc tgcctcgccg cacacattcc ccgcatgatc
     1861 ctgcgcaaag gcgaagaaga ggcggaaaaa gaattcaaat ggtggctcga tattttcggg
     1921 gaggattatt acatcgaact gcagcgccac gatattcccg accagaacaa ggtgaatgaa
     1981 gtgctgctga aatgggcagt gaaatacaac gtgaaagtca tcgcctccaa cgattcgcat
     2041 tacgtgaacg tggaggatgc gaacgcgcac gacatcctcc tttgcatcaa cacgggcgag
     2101 aagaaaagca cgccttcggc aaaggaattt tcggatgatg attcgtcgat gaaaggacgg
     2161 cgctttgcgt tctggaacga ccagttctat ttcaaaacga cgcaggaaat gtcggatctt
     2221 tttgcggacg ttccgcaggc gatcgacaac acgaacgaga tcgtcgacaa gatcgaaccg
     2281 ctgaagctga aacgcgacat ccttctcccc cactacaaaa tcccggaagg cttcaccgac
     2341 caggacgaat acctgaagca cctcacgttc gagggtgcgc gcagaaaata caaagagctc
     2401 acgcccgaga tcgaagagcg catcaacttc gagctgttca cgatccgcac catgggattc
     2461 gccggctact tcctcatcgt gcaggatttc atcaacgccg gcaagaacat cggcgtgctg
     2521 atcggtccgg gccgcggttc cgccgcgggc tccgttgtcg cgtactgcac cggcatcacg
     2581 aacatcgacc cgatcaaata caatttgctg ttcgagcgat tcctgaatcc cgaccggaaa
     2641 tcgatgcctg atatcgatac ggatttcgac gatgaagggc gacagaaagt gatcgattac
     2701 gtcgtcgaca aatacggccg caaccaggtg gcgcagatcg tcacctatgg aacgatggcg
     2761 gcgaaaatgt ccatcaaaga tgtggcgcgc gtgctcgatc ttccgctcga ccagtcgaac
     2821 gcgatggcga agcttgttcc cgaacgtccc ggcattgcgc tgggccgcgt gctcaccgca
     2881 ccgatggacg gagaaaaatc gctgcgcgag aaagagcagc tcggcggcga tgagctcgac
     2941 aacgtcagga aactgcgcgc tatatacggc ggcaacgatc tccaggccga agtgctgaaa
     3001 gaagcgctgg tgctcgaagg ttctgtacgc ggaacaggaa tccacgccgc gggcatcatc
     3061 atcgcaccgc aggatctcac ggacatcatt cccgtcgcga catcgaagga tacgaacctt
     3121 ttcatcacgc agtacgacgg gaaagtgatc gaagatgcag gcgttatcaa gatggacttt
     3181 ctcgggctga agacgctcac cattatccgc gacgcgctga agctgatcag gcaaaaccac
     3241 aacatcgaca tcgagatcga tgaaattccg ctcgacgacc cgaagacatt ttcactttac
     3301 cagcgggggg aaacgaacgg cacattccag ttcgagtcga acgggatgca gaaatacctg
     3361 aaagacctga agcccgacaa gttcgaggac ctcatcgcga tgaacgcgtt gtaccgcccg
     3421 ggtccgctgg aatacattcc gaaattcatc ctccgcaagc acggccgcga agcgatcacg
     3481 tacgatcttc ccgaaatgga agagttcctg aaagacacgt acggtattac ggtttaccag
     3541 gagcaggtga tgctgctttc gcagaagctc gccggtttca ccaaaggcga tgcggatatc
     3601 ctgcgcaaag cgatgggaaa aaaagaccgc gcgacgctgg acaagatgaa gggaaaattc
     3661 atggaaggca cgaaagcgaa gaacctcgat gccgccacct gcgaaaaagt gtggaccgac
     3721 tgggaagcgt tcgcgcaata cgcattcaac aaatcacact ccacgtgcta tgcattcgtc
     3781 gcgtaccaga cggcctacct caaagcgcat tatcccgcgg aatacatggc atcggtgctc
     3841 acgcacaacc tcggcaacat cgacaagatc acgttcttca tggaagaatc gcgcagaatg
     3901 ggaattcccg ttctcggacc ggacgtgaac gaatcggaga tccatttttc cgtcaacaaa
     3961 aaaggagaga tccgtttcgg catgggcgcg atcaaaggtg tgggcgaagg cgcagcacag
     4021 gcgatcatcg aagagcgcaa agcgaacggt ccgtataaaa acatttttga tcttgtccgc
     4081 cgcgtgaacc tgcgtgcagc gaacaagaaa acgctggaaa atctcgttta cgcgggagga
     4141 atggattcct tcacggatct gcaccgcgcg acgtatttca aaaatgagaa cggggaaaat
     4201 tcaccgacgt tcctcgaaaa aataatccgt tacgggcagg cgcagcagga aagcaggaat
     4261 tcctcgcagg tttcgctgtt cgacatgatg ggcgatgacg gcggaattga aatgccggaa
     4321 cccgctgttc ccaaagcgga agaatggggc acgcttgaaa agctgaaagc ggaaaaagac
     4381 gttatcggta tttacctttc cggtcacccg ctggatgatt tccgtacaga aatggtttct
     4441 ttctgccggc acaagctgtc cgatcttgcc gacctgccga aatggaagaa ccacgaaatg
     4501 catctcggcg gaatgatcac ctccgttcag cacaaaatga cgaaaacagg caagccgttc
     4561 ggcacgttcg tgctcgaaga ttacgattcc tcgtacgagt tcgcgttgtt cggcgaggat
     4621 tatgtccgct tcaagcagtt tatcctcacg ccgggccttt tcatcctgat gcgcggaaaa
     4681 gtgcaggaac gtttcggcaa ttccgaaaat ctcgaagtga aaattcattc gatggaattg
     4741 ctgagcgagc tgctggagaa aaaagtgaag caattcacga tccagcttcc cctgccctct
     4801 ctcagcgacg atctcattgc gcgcgtgcag gacatcatcg cgaaatatcc cggcaattct
     4861 gcgctgcgct tcaccgtgta cgaccaggcg gaaaacattc agctggaact tccctcgcgc
     4921 aaagtgcgtg tgaaacccgg gaatgagctg atcagggaac tcgaagcggt ggtgccggaa
     4981 gcggggtgga aattgaatta gacagtcaga cttcggtacg tccaccggag gcggactact
     5041 cagtctgact gtaaagaggg ttgcttccgg gctcatcgtc aaaaagaatg gactgctgtc
     5101 cttcgatacg tttcgcacct ggttgtcatc cctcggtacg tccgccggtg gcggacacgc
     5161 ggtctgacaa tgctttggtc agactgagta gtcggagcac acacttgaaa ttcttcgtcg
     5221 tcctccctcg atacgtccgc cgctggcgga cactcggtct gacaatgttt tggtcaggat
     5281 gagtagttgc aatacgcact tgaatttctt tgctgtcatc ccccggtacg tccaccgaag
     5341 gcggacactc ggtctgacaa tgctctggtc agactgagta gtcacgcaca gcgtgacgta
     5401 ccgaagtctg accactcaca ccaccttctt cttcacaaac ttctttttcg caagcatctt
     5461 caaatcatca actcttcctt ctatcaatgc ctcctttttg acgcgtgacc atcttttgat
     5521 ttgcttttcc cggcgaatgg caacacgaac atccctgaaa ttttcagacc acacaagctc
     5581 gaccggcaat cgctcagagg tataagagcc ttcattactt gcattcacat gttcacgcaa
     5641 ccttcttcgg agattatttg tcacgcccgt gtaataggaa tcatctgcgc atttcaaaat
     5701 gtaaaccgaa tattttctca tatctcaaaa aacaaaaaat caaaaacggg agcgaaaata
     5761 aaaattattg gaatgcagtt tcccctgaga tgtgggaata cgcggcgcca tcccttgata
     5821 cgctctacaa atggttgtca tccctcggta cgcccgccac tggcggacac tcggtctgac
     5881 aatgttttgg tcagactgag tagtcggagc acgcgcttga aattctttgc tgtcatccct
     5941 cggtacgtcc gccgctggcg gacactcggt ctgacaacgc tttggtcagg atgagtagtc
     6001 gcagcacaca cttgaaattc ttcgtcgtca tccctcggta cgtccgccgc tggcggacac
     6061 tcggtctgac aatgctttgg tcagactgag tagcctgcca aaggcagacg taccgaagtc
     6121 tgacctctac cccaacagcg tctccttcgt tttcgcaaga atatcaaacg cttcctcgcg
     6181 tgtcgccgct tccgcgtagg tacgcagcac gggctcggtt ccgctggcgc ggatcatcac
     6241 ccaggtctcg tcattgagga aaaatttcca gccgtcgaga tcttcgacgt gatcgacttt
     6301 atactttccg aatgttttgt atctccccgc tttgcaatcg gcgatgacgc gctgtttcac
     6361 gctttcgtcg agatgcagat cgacgcgctc gaaggaaaac ggaccggtga tcgcatacac
     6421 ttcctcgatc agctgcttca gcgttttgcc gctcttcgcc atgaattccc agatgacgag
     6481 ccccatccag atgccgtcgc gttcgggaat gtgccctttg atcgcgatgc cgcctgactc
     6541 ttctccgccg agcagcacgt cttccgtcac catgatgccg cagatgtatt tgaaaccgat
     6601 cttcaccgtt tccagctcga gaccataatg gcggcacatg tttttgatct tcaccgtcgt
     6661 gctgaatgcc gtgcacacct tgcctttcat gcctttgtat ttcacgaggt agtgtatgag
     6721 cagcaggatg atgtggtgcg agtcgacgaa atttccttcg ttgtcgaaaa gaccgatacg
     6781 gtcggcgtca ccgtcggtgg cgagcccgca gtcgatgtct cccgccgtcc tgatcacgtc
     6841 ggcgaattcc tgcaggttac ggagaatagg ttccggcgcc tgcccgtcga aagaaggatt
     6901 gtattcgcaa tgcagcatcg tcgtgtcggg gaacaggcgg cggatcacgt tctgccccgc
     6961 gccgtacatc gcgtcgtaag cgaggt
//
LOCUS       DASTSF010000303         3786 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1615907,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000303 DASTSF010000000
VERSION     DASTSF010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3786)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3786)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3786
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1615907"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1650
                     /locus_tag="VFU15_08750"
     CDS             <1..1650
                     /locus_tag="VFU15_08750"
                     /inference="COORDINATES: protein motif:HMM:NF018000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkA"
                     /protein_id="HEU4717911.1"
                     /translation="VRGVMLLVTIGGIFILSTLIGTLTAGLEAKLEELRKGRSKVLEN
                     GHTLILGWSPKIFTIISELLIANENKHKPRIVILADKDKVEMEDEIKAKFPSTKNTKV
                     ICRTGSPLDLDDLEVVSPHDAKSIIVLTPEDTGSPDTFVVKSILALTNNPKRKSEPYH
                     IVAELRDEKNMEAAELVGGKEAAVMLSEDIIARVTAQTCRQSGLSVVYTELMDFDGAE
                     IYFSAEKALNGKTYREIISAYEDSAVMGLMRKSGELLINPPMETVYEDGDQVIAITED
                     DDSLVIGKTAPPAPDHSAISIGEREKPGVERTLILGWNKKGPAIIRELDNYVAPGSEV
                     MVVSEDGDSEEETKDLASTLSRQKLTYRHANITEKAVIHSLDVPSFHHVIVLCYSHLD
                     VQEADAQVLITLLHLRNIAESSGKRYGIVSEMRDIRNRALAEVARADDFIVSDKLISL
                     MLSQVSENKHLEAVFKDLFRSEGSEIYLKPAKEYVKTGTAVNFYTVLEAAAKRNETAL
                     GYRITSLANDPEKAYGVVVNPNKSKSITFGDEDKLIVLAED"
     gene            complement(1734..3311)
                     /locus_tag="VFU15_08755"
     CDS             complement(1734..3311)
                     /locus_tag="VFU15_08755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717912.1"
                     /translation="MKKRIFFLLIASFFAGKHLHAQVIAESDPKTAADKFKAHNYDEA
                     LVDYLDLLEDDPKNMDYNYRIGVCYLNTNVNKAKAIPYLEVVTRQDKYEPDAMYLLGR
                     AYHFAYRFDDAIRCYNTFKTNGKGTAENLADVDREIQNCYNAKELMKYPLNVTFENLG
                     SSLNSPFPDYYPFVPADESFIVYNTKRSDNGNFRQPDGSYYSTVYISRESDGGFLKGK
                     NIGAPIATDDGNVEVCGLSANGNYLLLYYDNDQETGDIYISEWDKTKNMYKKPVRLDD
                     NINSTKGYEIAATITNDGNTIYFASDRPGGYGGMDIYVCNKLPNGKWGPAKNLGPEIN
                     TAYDEDFPSLSPDGKTLYFSSKGHSSMGGYDIFKTDYDDATANFTGVKNLGYPINTPE
                     DNSNFRISENGRYGYVAMRRDDGLGDLDIYRVTFNDVEPNYSVVAGQIRFSDETKKIP
                     YNEVFITVTNHKTNEIVGNYVPNGNTGRYIMILGPGDYDVSIDAPGFAAYAETINIFD
                     KSSFKPEIDKDFQLKAQ"
     gene            3414..>3786
                     /locus_tag="VFU15_08760"
     CDS             3414..>3786
                     /locus_tag="VFU15_08760"
                     /inference="COORDINATES: protein motif:HMM:NF019236.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HEU4717913.1"
                     /translation="MKKWITVLFLSVPGFLSIEAQQLQSLYVSMDGDNSNPGTLTHPF
                     RTIQHALDQAQAGTAIFVMAGTYAETLTASVTGTAAQPVSLKNYSGQNAIIDGAYATG
                     APLLSINNASYFTVSGLQFSGY"
BASE COUNT          895 a          984 c         1023 g          884 t
ORIGIN      
        1 gtgcgcgggg tgatgctgct ggtaacgatc ggtggaattt tcatcctcag caccctcatc
       61 ggtacgctga ccgccgggct ggaagcaaag ctggaagaac tgcgcaaggg acggtcgaaa
      121 gtgctcgaaa acgggcatac gctcatcctg ggctggtcgc cgaagatatt caccatcatt
      181 tccgagctgc tgatcgccaa cgagaacaag cataaaccgc gcatcgtcat tctcgccgac
      241 aaagacaagg tggaaatgga agacgagatc aaggccaagt tcccttcaac gaagaacacg
      301 aaggtgatct gccgcacggg aagcccgctc gacctggacg atctcgaagt ggtcagtccg
      361 catgacgcca aatcgatcat cgtgcttaca ccggaggaca cgggcagccc ggacactttc
      421 gtcgtcaaat ccattcttgc cctcaccaac aacccgaaac ggaaaagcga gccctatcac
      481 atcgtggccg aactgaggga tgaaaagaac atggaagcgg cggagctggt gggtggaaaa
      541 gaagcggctg tcatgctttc cgaagacatt attgcccggg tcaccgcaca aacctgccgg
      601 cagtcgggac tgagcgtcgt ctatacggag ctcatggatt tcgacggtgc ggaaatttat
      661 ttcagcgcgg aaaaagcgct gaacgggaaa acctaccgtg agattatttc ggcttacgaa
      721 gattcggcgg ttatgggact catgcggaaa agcggtgagc tgctgatcaa tccacccatg
      781 gaaaccgttt acgaggacgg agaccaggtt attgccataa ccgaggacga tgattcgctt
      841 gtcattggaa aaacggcccc gccggcgccc gatcattctg ctatttccat tggtgaacgt
      901 gaaaaaccgg gtgtagagcg tacgcttatt ctcggctgga ataaaaaagg accggccatt
      961 atccgggaac tcgacaatta cgtcgcgccc ggatcagaag tgatggtggt gagcgaagac
     1021 ggcgattccg aggaggaaac aaaagactta gcctccactc tttcccggca gaagctgacc
     1081 taccggcacg ccaacattac cgagaaagcc gtgatccatt cgctggacgt tccctccttt
     1141 catcatgtca tcgtgctttg ctacagtcac ctcgacgtgc aggaagcgga tgcgcaggtg
     1201 ctcatcacgc tgctccacct gcggaacatt gcggaatcgt caggtaaacg ttacggcatt
     1261 gtaagcgaga tgcgcgacat acgcaaccgt gcgctggccg aagtggcgcg cgcagatgat
     1321 ttcattgtca gcgacaagct catcagtctc atgctttcgc aggtgtcgga gaacaaacac
     1381 ctcgaggccg tgttcaagga ccttttccgt tcggaaggtt cggaaattta ccttaagccc
     1441 gcaaaggaat acgtaaagac aggcacagcg gtgaattttt ataccgtact cgaagcggca
     1501 gcgaaacgaa acgaaaccgc gctcggctac cgcatcacct cgctcgcgaa cgatcccgaa
     1561 aaagcgtatg gcgttgtcgt gaacccgaac aaatcaaaaa gcataacatt cggggatgag
     1621 gacaagctga tcgtgctcgc ggaggattga tccctgtaaa tccccaatga aattccggat
     1681 ccggacgtac ggtaagcgac cgttcactcc cgcttggaat ccgggtccgt taattactgc
     1741 gccttcagct ggaaatcctt gtcgatctcg ggtttgaaag agcttttgtc gaaaatgttg
     1801 atcgtttccg cgtaagctgc gaacccgggc gcatcaatgg aaacatcgta gtcgccggga
     1861 ccgaggatca tgatgtagcg tcccgtgttt ccgttgggaa catagttgcc gacgatttcg
     1921 ttcgttttgt gattggtgac ggtgatgaac acttcgttgt acgggatctt cttcgtttcg
     1981 tctgaaaacc ggatctgccc ggccacgacc gaataattcg gctcgacgtc gttgaacgtc
     2041 acgcggtaaa tatcgagatc accgagcccg tcgtcgcggc gcatggcgac atagccgtaa
     2101 cggccgtttt ccgagatgcg gaaattgctg ttgtcttccg gcgtgttgat cgggtagccg
     2161 agatttttca caccggtgaa attcgctgta gcgtcatcgt aatcggtttt gaaaatatcg
     2221 tacccaccca tgctcgagtg acccttggag ctgaagtaaa gcgtttttcc gtcgggagaa
     2281 aggctgggga aatcttcatc gtaagccgtg ttgatctccg gcccgagatt tttcgcaggt
     2341 ccccatttcc cgttcggaag cttgttgcac acataaatat ccattccgcc gtaaccgccg
     2401 ggccggtcgc tggcgaaata aatagtattg ccgtcattcg tgatcgtggc cgcaatctcg
     2461 tatcctttcg tggaattgat attgtcgtcg agacgaacgg gctttttgta catgttcttc
     2521 gttttgtccc attcggaaat gtagatgtcg ccggtctcct ggtcgttatc gtaatacagc
     2581 agcaggtaat tcccgttggc ggaaagtccg cacacttcca cgttgccgtc gtcggtggcg
     2641 ataggagcgc cgatgttctt tcctttcagg aagccgccat cgctttcgcg ggagatgtaa
     2701 acggtggaat aatagcttcc gtcgggctgc cggaaattcc cgttatcgga acgtttggtg
     2761 ttataaacga tgaacgattc atcggccgga acgaagggat aataatccgg aaaaggagaa
     2821 ttcaggctgc tgccgagatt ttcgaacgtc acgttcagcg ggtacttcat caattccttt
     2881 gcgttgtagc agttctggat ctcacggtcg acatccgcca ggttttccgc cgttcccttc
     2941 ccgttcgtct tgaacgtatt gtagcaccgg atggcatcgt cgaaacggta ggcaaaatga
     3001 tatgcgcgcc cgagcaggta catggcgtcg ggttcgtatt tgtcctgtcg cgtaaccacc
     3061 tcgaggtagg gaatcgcctt cgccttgttg acattcgtgt tcaggtagca cacgccgatg
     3121 cgataattgt aatccatgtt tttcggatca tcttcgagga gatcgaggta atcgacgagc
     3181 gcctcgtcgt agttgtgcgc cttaaacttg tcggcggccg ttttggggtc cgattcggcg
     3241 atgacctgtg cgtgcaggtg cttcccggcg aaaaaggacg caataagcag gaagaaaatc
     3301 cgttttttca tgggtaaaaa atcaagggcg caaagttatg aaaatgccca ctcctcttct
     3361 gcatgttcgc ttatttcgac taattttacc tgtccccaaa ccctgttttg ccaatgaaaa
     3421 aatggattac cgttcttttc ctctccgtac ccggcttcct ttcgattgaa gcgcagcagt
     3481 tgcagagcct ttacgtaagc atggacggag acaattcgaa tcccggaacg ctgactcatc
     3541 ctttccgtac gatccagcat gcgctcgacc aggcgcaggc gggaactgcg atcttcgtga
     3601 tggcaggaac gtatgctgaa acgctcacgg catctgttac gggtacggca gcacaaccag
     3661 tttcgctgaa aaactacagc gggcagaacg ccatcatcga cggagcgtat gccacaggtg
     3721 ccccgcttct ttccatcaat aacgccagtt actttacggt aagcggcctg cagttttccg
     3781 gctacc
//
LOCUS       DASTSF010000304         2157 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1345725,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000304 DASTSF010000000
VERSION     DASTSF010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2157
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1345725"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..361)
                     /locus_tag="VFU15_08765"
     CDS             complement(<1..361)
                     /locus_tag="VFU15_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007376733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717914.1"
                     /translation="MLQFFWKLLVRMKNPTCTIQAETLTRNVSFEDKVTIEKGCFIGA
                     DKIGRYTFIGMNSYVDKSTASIGRFCSIAMGAKIGMRNHPMEWVSTHPFTYNRKYGFV
                     PQNVQIEGVNDRKTVIGN"
     gene            complement(368..1669)
                     /locus_tag="VFU15_08770"
     CDS             complement(368..1669)
                     /locus_tag="VFU15_08770"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEU4717915.1"
                     /translation="MKKILVVTYYWPPSGGPGVQRWLKFVKYLRRSGFGAVVVTVDPE
                     KASYPVFDHSLEKEIPEGTEVHRTNSFEPLRIFSSFFKKEKVPYAGIPDRDKMSLAGK
                     ISLYIRANFFIPDARKGWNRYAFRECCRIIEQEKITAVITSSPPHSTQLTGLRLKQKY
                     GIRWIADLRDPWTDIYYYHKLHHSERAKRKDLAYELSVLENADHILVTSNRTAKQYSA
                     KSAAVAGKIHVIPNGFDEDDFQTEAPASPGKFTVTFIGTINEQFGIGKFVAAVKNAQR
                     SLPSVPFEVKFVGQTDRAVENLLRSELGEMLVMPGYVSHKESVEEMRKAQLLLLVIPQ
                     GPNQGTVPGKTFEYLAAQRSIICLAPEGSSAADIIRSCEAGRSFVHDDQKGMEEYIVS
                     LAKEWQAKGDLKINNGNFRQYSRRSQAEILAGLISGATPKT"
     gene            complement(1666..>2157)
                     /locus_tag="VFU15_08775"
     CDS             complement(1666..>2157)
                     /locus_tag="VFU15_08775"
                     /inference="COORDINATES: protein motif:HMM:NF021126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HEU4717916.1"
                     /translation="DAWFVSNVKMVANPDSEITAVKTFNPASTAVVDKVFQDKLGDVK
                     TAPDPNSSIRLTSYEPNDLKYESNSSSEQFAVFSEVYYDKGWNAYIDGKEAPYVRADF
                     VLRAMKVPAGKHEIEFKFEPSFYYTGEKIALAGSILLFLMVGGGIYAQSKKMKKEAEN
                     EAA"
BASE COUNT          455 a          584 c          561 g          557 t
ORIGIN      
        1 cgttcccgat caccgtttta cgatcattca caccttcgat ctgcacgttc tgcgggacaa
       61 aaccgtattt ccggttgtag gtaaacgggt gtgtgctcac ccactccatc gggtgattgc
      121 gcatcccgat cttcgcgccc attgcaatgg aacagaaacg tccgatcgac gcggtgcttt
      181 tgtccacgta gctgttcatg ccgatgaacg tgtagcgccc gatcttgtcc gcgccgatga
      241 agcatccttt ttcgatcgtc actttgtctt cgaacgaaac gttgcgcgtg agcgtttccg
      301 cctgtatcgt gcacgtggga tttttcatcc gcaccaggag tttccagaaa aactgcagca
      361 tgacgtttca ggttttcggt gttgcgccgg aaataagacc ggcaagaatt tcggcctggc
      421 tcctccggga atactgccgg aagttaccgt tatttatttt caggtcgcct tttgcctgcc
      481 attccttcgc cagcgaaacg atgtactctt ccatcccttt ctgatcatcg tgcacgaaag
      541 accggcccgc ttcgcatgaa cggataatgt ccgctgcgct gcttccttcg ggcgcgagac
      601 aaataatgct gcgctgcgcc gcaaggtatt cgaacgtctt tcccggaacg gttccctggt
      661 tcgggccttg cggaataacg agcagcagca gttgtgcctt ccgcatttcc tccacggatt
      721 ctttgtgcga aacataaccc ggcatgacga gcatttcgcc gagctccgat cgcaggagat
      781 tttccacggc acggtcggtc tgtcccacga atttcacttc gaaaggaacg gacggcagcg
      841 aacgctgcgc attcttcacg gcagcaacaa atttcccgat gccgaactgt tcgttgatcg
      901 taccgatgaa cgtgaccgtg aattttcccg gggacgcagg agcttccgtc tggaaatcgt
      961 cttcgtcgaa accattggga atgacgtgta tcttcccggc gacagccgcc gatttggcgg
     1021 aatactgttt cgccgtccgg ttgctggtaa caagaatgtg atccgcattt tccagcacgg
     1081 acagctcgta cgcgaggtct tttctcttcg cgcgttcgga atgatgcagc ttgtggtaat
     1141 agtaaatatc cgtccaggga tcgcgcaggt cggcgatcca ccggatgccg tatttctgtt
     1201 tcaggcgcag cccggtcagc tgcgtggaat gcggcgggct cgaagtaata accgcggtta
     1261 ttttttcctg ttcgataatg cggcagcatt cacggaatgc atacctgttc catcctttcc
     1321 gcgcgtcggg aataaaaaaa ttcgcacgta tgtaaagtga tatttttccc gccaggctca
     1381 tcttgtcccg gtcgggaatg cctgcgtacg gtactttttc ctttttgaag aacgaagaaa
     1441 atatacgcag gggctcgaaa gaattcgtcc ggtgaacttc cgttccttcg ggaatttctt
     1501 tttcaaggga atgatcgaaa acgggataag aagctttttc cgggtcgacg gtaaccacga
     1561 cggcaccaaa accggaacgg cgcaggtact tcacgaattt cagccagcgt tgcaccccgg
     1621 gtccgccgct cggtggccag taataggtta caacgaggat tttcttcatg cagcttcgtt
     1681 ctctgcctct ttcttcatct ttttgctctg cgcgtaaatg ccgccgccca ccatcaggaa
     1741 gaggagaatg gacccggcca gcgcaatttt ctcaccggtg taatagaaag aaggttcgaa
     1801 tttgaattcg atctcgtgct tgcccgcagg gactttcatc gcgcggagaa cgaaatcggc
     1861 gcgcacataa ggagcttctt ttccgtcgat gtaggcgttc catcctttgt cgtaatagac
     1921 ttcggaaaaa acagcgaact gttcggaaga agaattcgat tcgtacttca ggtcgttcgg
     1981 ttcgtacgat gtaaggcgga tggacgaatt cggatcggga gcggttttca catcacccag
     2041 cttgtcctgg aaaaccttgt cgacgacagc ggttgacgcc gggttgaatg ttttcaccgc
     2101 ggtgatctcg ctgtccgggt tcgccaccat cttcacgttg ctgacaaacc acgcgtc
//
LOCUS       DASTSF010000305         3688 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_669066,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000305 DASTSF010000000
VERSION     DASTSF010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3688
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_669066"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..350)
                     /locus_tag="VFU15_08780"
     CDS             complement(<1..350)
                     /locus_tag="VFU15_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013632908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpX C4-type zinc finger protein"
                     /protein_id="HEU4717917.1"
                     /translation="MATKETNIHCSFCGRDKQDTLVLIAGIQGHICDQCIQQAYRIVK
                     EESDAKKTQALNSALTLLKPVEIKRHLDEYVIGQEQAKKVLSVAVYNHYKRIAQKPSK
                     KATEEIEIEKSNIVL"
     gene            complement(422..1099)
                     /gene="clpP"
                     /locus_tag="VFU15_08785"
     CDS             complement(422..1099)
                     /gene="clpP"
                     /locus_tag="VFU15_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HEU4717918.1"
                     /translation="MSDHNEFRKYAVKHMGINSLTLDHYASVTPRGMTPMIIEDRPVT
                     GTSMSVFDRLMIDRIIFMGTAINDQVANIIQAQLLFLESSDPKKDIQIYVNSPGGSVY
                     AGLGIYDTMQLIQPQVATICTGMAASMGAVLQCAGAKGKRSALPHARVMIHQPLGGAE
                     GQASDIEITAREIMKLKKELYDIIAHHSGQTYDKVWADSDRDYWMTAQEAKDYGMIDE
                     ILVRRNK"
     gene            complement(1172..2518)
                     /gene="tig"
                     /locus_tag="VFU15_08790"
     CDS             complement(1172..2518)
                     /gene="tig"
                     /locus_tag="VFU15_08790"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEU4717919.1"
                     /translation="MDITKEQIDDLNAVVKVKVGPADYNEKVEKALRSYQKKVSVPGF
                     RPGKVPSSLVKKMYGKAVLAEEINRILSDSLYNYIRENNIEVLGNPLPKEEVNDKVDF
                     DKQTDFEFHFDMALAPQFSLALGADMNFTEYVVRPDDKLIDNYVNDMARRYGRISAVE
                     AAEAGDLLHGDFVELDENGEIVPGGIFKSSTVFLDNPVKEIHQELIGAKTGDKFDLTP
                     AQISDNPKDLAGKLGVDENKVQELKNKFRFTVRNVSRLVPAELNEELFDKIYGPGVVT
                     SPEQFREKIAEELRGMFTRDTEARLKNDIAAELLNRANLSLPDAFLKRWLLAANEKPL
                     TQEQIDAEYPLYSRQLRWQLIENKLIRDNGISVTAEEAEEHVKQILRANMAKYGQQPD
                     SLKDEELTATAKRVLQKEEEAKKVFEDLYAAKLMMLYRTKCTITPREVTYEQFLAA"
     gene            2809..3192
                     /locus_tag="VFU15_08795"
     CDS             2809..3192
                     /locus_tag="VFU15_08795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717920.1"
                     /translation="MAKEAMLASVRMVYADDGILYLTILPNARIDVKHVQAQNDCIRN
                     LCGDTRVPVLLDARVDYTVTKEAQEYAAMNSNRLATAVLVSSPVVKALTNTFVALFRP
                     DYPVRQFTSEEPAVAWLKEIMRGKK"
     gene            complement(3269..3655)
                     /gene="apaG"
                     /locus_tag="VFU15_08800"
     CDS             complement(3269..3655)
                     /gene="apaG"
                     /locus_tag="VFU15_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003009688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HEU4717921.1"
                     /translation="MQTLVTHGIKICVESTYQDLYSRPEQGHYYFSYRIRIENNSSAT
                     VQLMRRHWYIFDSCGEHSEVEGEGVVGQQPVLRPGESYEYESACHLTTEIGSMHGTYE
                     FVHVDDKRSFEVIIPRFLLIAGHRLN"
BASE COUNT          774 a          950 c         1002 g          962 t
ORIGIN      
        1 agcacgatgt tcgacttttc gatctcgatc tcttcggtcg ctttcttcga aggcttctgc
       61 gcgatgcgtt tgtaatgatt gtaaacggcg acggaaagaa cttttttcgc ctgttcctgc
      121 ccgatcacgt actcgtcgag gtgacgtttg atctcgaccg gcttgaggag cgtaagcgcg
      181 gaattcagcg cctgcgtttt cttcgcatcc gattcttcct tcacaatgcg gtacgcctgc
      241 tggatgcact ggtcgcagat atgaccctgt atgcccgcaa tgagcacgag cgtatcctgc
      301 ttgtcgcgac cgcagaaaga acagtggata ttggtttctt ttgttgccat tccgggaaaa
      361 agtttcgaat tccggattct aagttctgaa tttagaaccc ggaattcaaa gctgccaact
      421 attatttgtt ccggcggacg aggatctcgt cgatcatgcc gtaatctttc gcttcctgcg
      481 ctgtcatcca gtaatcacgg tcgctgtcgg cccagacctt gtcgtaggtc tgcccgctgt
      541 ggtgcgcgat gatatcatac agctcttttt tcagcttcat gatctcgcgt gcggtgatct
      601 caatgtcgga cgcctgtcct tcagctccgc caagcggctg gtggatcata acacgggcgt
      661 gcggaagcgc ggaacgcttt ccttttgcac ctgcgcactg gagcacggcg cccatcgaag
      721 cggccatgcc cgtgcagatc gtcgcgacct gcggctggat cagctgcatc gtgtcgtaaa
      781 tgccgagccc ggcgtaaacc gaacctccgg gagaattcac gtagatctgg atgtcttttt
      841 tcggatcgga tgattcgagg aaaagcagct gggcctggat aatgttcgcg acctggtcgt
      901 tgatggccgt tcccatgaaa atgatacgat cgatcatgag acggtcaaac accgacatgg
      961 aggtgccggt gacggggcgg tcttcgatga tcatcggggt catgccccgc ggtgtcacgg
     1021 aagcatagtg gtcaagcgta aggctgttga tgcccatgtg cttgacggca tatttgcgga
     1081 attcattgtg atctgacata aaaatgaaat atggttatgt ggtgaaatta atcaagatcg
     1141 gtgccacccg caatttactg ccacaatggc gtcacgccgc caggaattgc tcgtacgtca
     1201 cttccctggg tgtaatggtg catttggtgc ggtagagcat catgagcttc gctgcataaa
     1261 gatcctcgaa cactttcttc gcttcttcct ctttctgcag cacgcgtttg gcggtagcgg
     1321 tcagctcttc atccttgagc gaatcgggtt gctggccata ttttgccatg ttggcgcgga
     1381 ggatctgctt cacatgctct tcggcttctt cggcggtaac ggaaatgccg ttatccctga
     1441 tgagcttgtt ctcgatcagc tgccagcgga gctgccggga ataaagcggg tattccgcgt
     1501 cgatctgttc ctgcgtaagc ggtttttcat tggccgccag cagccagcgc ttcaggaaag
     1561 catccggaag cgaaaggttg gcgcggttca gcagctctgc cgcaatgtcg tttttcagcc
     1621 ttgcttccgt atcgcgggtg aacatgccgc gcagctcttc cgcaattttt tcgcggaact
     1681 gttcgggcga tgtcacaacg ccgggaccat agatcttgtc gaacagctct tcgttcagtt
     1741 ctgcgggaac gagacggctg acgttgcgca cggtgaaacg gaatttgttc ttcagctcct
     1801 gtaccttgtt ttcatccacg ccgagcttgc cggcaagatc tttcggatta tccgagatct
     1861 gggcaggcgt gagatcgaac ttgtcgccgg tctttgcacc gatgagctcc tggtggatct
     1921 ctttcaccgg gttatcgagg aatacggtcg atgatttgaa aatgccgccg ggaacaattt
     1981 ccccgttctc atccagctcg acgaaatcgc cgtgcaggag atcgcccgct tcagcagctt
     2041 ccactgcgga aatgcggcca taacggcgcg ccatgtcatt gacgtaatta tcgatcagct
     2101 tatcatcggg gcgaacgacg tattccgtga aattcatgtc tgcgccgagc gcgaggctga
     2161 actgcggcgc aagcgccata tcgaaatgga attcgaaatc cgtctgcttg tcgaaatcga
     2221 ccttgtcgtt cacttcctcc ttcggaagcg ggttgccgag cacctcgatg ttgttttcac
     2281 ggatgtagtt gtagagggaa tcggaaagaa tgcggttgat ctcttcggca agaacggctt
     2341 tgccgtacat ttttttcacg agcgaggaag gcacttttcc gggacggaac ccgggcacag
     2401 aaaccttttt ctggtagctg cgcagcgctt tttccacttt ttcgttgtaa tcggccgggc
     2461 cgacctttac tttgacaacg gcattgagat cgtcgatctg ttctttggtg atgtccatct
     2521 taactaactg gtaatgagtt tgtttaaaag tataccccat taaatgggga cggcaaagat
     2581 acggaaaatc atgcgtaaag gggtgaatgt acgccgtatt caggatgcag gaagaccggg
     2641 cgtttgattc ggaatttagg cgcctgcgcg cccgcaattc cattttcccg gctgcgtccg
     2701 tgagataacc atttacaaaa gtaaagtgcc ctgttaggaa acaaccggcg cctctttctt
     2761 ttttcagcac ttactttgcc ctctttttac atcccaataa ttgaaataat ggcgaaagag
     2821 gcgatgcttg catccgtgcg gatggtttac gccgacgatg gaattcttta tctcacgatc
     2881 ctgcccaatg cgcgcatcga tgtgaagcat gtacaggcgc agaatgactg catccgtaac
     2941 ctgtgcggcg atacccgcgt gcctgttctt ctcgatgcac gtgtggacta cacggttacg
     3001 aaagaagcgc aggaatacgc ggcgatgaat tccaatcgtc ttgcgacggc tgttctcgta
     3061 tcgagtcccg tggtaaaagc gctcacaaat acattcgttg cgttgttccg tcccgattat
     3121 cccgtacggc agtttaccag cgaagagccg gcggtggcgt ggctgaagga aattatgcgc
     3181 ggaaagaaat aagctttgcc gtgccgcatt ccccggcgcg ggcaaaatcc attacagcga
     3241 ttccggaacc tggattgtaa acagccgctc agttgaggcg gtgaccggcg atcagcagga
     3301 agcgggggat gatcacttcg aaggaacgtt tgtcatcgac gtgaacgaac tcgtaggtgc
     3361 cgtgcatgct gccgatctcg gtggtgagat ggcacgcgct ttcgtactcg taggattctc
     3421 cggggcgaag cacaggctgc tgcccgacaa cgccttctcc ttccacttca ctgtgctctc
     3481 cgcaggaatc gaaaatgtac cagtgccgtc gcatcagctg cacggtagcg ctgctgttat
     3541 tttctatccg gatgcggtag gaaaaataat agtgcccctg ttcgggccgt gaataaagat
     3601 cctggtatgt actttccaca cagattttga ttccgtgcgt gaccaacgtt tgcatcttat
     3661 ccggagtttg cctacacaag tttaggac
//
LOCUS       DASTSF010000306         2409 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1534345,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000306 DASTSF010000000
VERSION     DASTSF010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2409
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1534345"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..452
                     /locus_tag="VFU15_08805"
     CDS             <1..452
                     /locus_tag="VFU15_08805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717922.1"
                     /translation="EYMDKHWDLLGWSLLPDQYNLVVQVRDVGDDTRSWLEINREAVA
                     DFSHFTNGYAKYINRRYDRRGSLFARNFCRSLIPSRSWLVHTIVATHARPLQASLCAD
                     LREWPHSSFIKMHLYSDDPLRAKLVRLFNGESEFFRQHSHFIQRIAA"
     gene            complement(480..2321)
                     /locus_tag="VFU15_08810"
     CDS             complement(480..2321)
                     /locus_tag="VFU15_08810"
                     /inference="COORDINATES: protein motif:HMM:NF013560.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M2 family metallopeptidase"
                     /protein_id="HEU4717923.1"
                     /translation="MKNKRILFLLPLAAVFFLPLSCKNNPAEDDATFVKRVDSFLVSY
                     NNKYRELTITSNEAVWKSLTHMQEGDTMADYLSEKAQNDLARFQGDSTIIHMTQQFLA
                     KKDKLNELQLEQLEHILYFAGGSPQTAGDLVGKKIAAETEATKMLYNFPFRIDGKEVT
                     PNDIDDILITERDTVKRLKAWEASKEVGDSLHKPLDNLRNLRNQVVQALNYPDYFSYQ
                     VADYGMSADTMMSMLRQINDEIYPLYRELHTWARYELAKKYGSPVVPDYIPAHWLPNR
                     WGQDWSDLVTVKGVDLDSIITARKFDAKWEVQEAERYYVSMGFPQLPQTFWDKSDLYP
                     PPPGADYKKNNHASAWHMDLDQDVRSLMSLEPNAHWYETTHHELGHIYYYLSYSRPEV
                     PFILREGANRAFHEALGTMFGMAAMQQPFLQGLGFVDSTVKIDTMQALLKEALRYVVA
                     LDWQAGTMSEFEYELYAKNLPADHFNQRWWEIVKQEQGIVPPYFRGENFCDPATKTHI
                     IDDPAQYYDYAFASVLMFQFHDYIAKNILHQNPRATNYYGNKQIGQFIGSMMSVGGVG
                     DWRTLLKDKTGEDLSAKPMLDYFAPLMDYLKKVNAGRKYTLPEKRVR"
BASE COUNT          525 a          617 c          641 g          626 t
ORIGIN      
        1 gtgagtacat ggataagcac tgggacctgc tggggtggag tttactgccg gatcagtaca
       61 accttgttgt tcaggtacgt gatgttgggg acgacacgcg gtcctggctg gaaatcaatc
      121 gggaagctgt ggccgacttc agccatttta cgaatggata cgcgaagtac atcaacagga
      181 gatacgacag gaggggcagt ttgtttgcga ggaacttttg ccgctcgctg attccttcgc
      241 gtagctggct ggtacatacc attgtcgcca cgcacgccag gccgctgcag gcttcgctgt
      301 gcgccgacct tcgcgaatgg cctcactcgt cgttcattaa aatgcatttg tattcggatg
      361 atccgctgag agcaaagctg gtccgtttgt tcaacggcga atccgaattc ttcaggcagc
      421 attcccactt cattcagcgg atcgctgcat gagcgcataa aagtaaaagc ggcggagggt
      481 cagcgcaccc gtttttccgg cagtgtatat ttccttccgg cattgacctt tttcaggtaa
      541 tccatcagcg gagcgaaata atcgagcatc ggtttggcgc tgagatcctc gccggttttg
      601 tccttcagca gcgtcctcca gtcgccgact ccgccaacgc tcatcatgct gccgataaat
      661 tgcccgatct gcttgttgcc ataataattc gtggcacgcg gattctgatg caggatgttc
      721 ttcgcgatat aatcatggaa ctggaacatc agcaccgacg caaacgcgta atcataatat
      781 tgcgcaggat cgtcgataat gtgcgttttc gtcgcgggat cgcagaaatt ctctccgcga
      841 aaatacggcg gaacgattcc ctgttcctgc ttgacgatct cccaccagcg ctggttgaaa
      901 tgatcggccg gaagattttt tgcgtacagc tcgtattcga attcgctcat cgttccggcc
      961 tgccagtcga gcgcgacgac atagcgcaat gcttccttga gcagcgcctg catcgtatcg
     1021 atcttcaccg ttgaatcgac aaagccgagt ccctgcagaa aaggttgctg catcgccgcc
     1081 atgccgaaca tcgttcccag cgcttcatga aaagcgcggt tggcgccttc gcggagaatg
     1141 aacggaactt ccggccggct gtaagaaaga taataataga tatgcccgag ttcatggtgc
     1201 gtcgtttcat accagtgcgc attcggttcg aggctcatga gtgaacgcac gtcctggtcg
     1261 aggtccatgt gccacgcgga agcatggttg ttttttttat agtcggcgcc tggcggcggc
     1321 ggatagagat ccgacttgtc ccagaatgtc tgcggcagtt gcggaaagcc catgctcacg
     1381 taatagcgtt ctgcttcctg cacttcccat ttggcatcga atttccgcgc tgtgatgatg
     1441 ctgtcgagat cgacgccttt cacggtaaca agatcgctcc agtcctggcc ccagcggttc
     1501 ggcagccagt gtgccggaat gtaatcgggc acgaccggtg atccgtattt tttggccagt
     1561 tcgtagcgcg cccacgtatg aagttcgcgg taaagcgggt agatctcgtc gttgatctgg
     1621 cgcagcatcg acatcatcgt gtcggcgctc atcccgtaat cagcgacctg gtaggaaaaa
     1681 taatcaggat agttcagcgc ctgcacgacc tggttgcgca ggttgcggag attatcgagc
     1741 ggtttgtgca gcgaatcgcc cacttccttg ctcgcttccc atgctttcag tctttttacc
     1801 gtgtcgcgtt cagtgataag tatgtcgtcg atgtcattcg gcgttacttc tttcccgtca
     1861 attctgaacg ggaaattgta cagcattttt gttgcctccg tttccgcagc gatctttttt
     1921 ccgacgaggt cgccggctgt ctgcggactt ccgcccgcga aataaagaat gtgctcgagc
     1981 tgttcgagct gcaattcatt cagcttgtct tttttggcga ggaactgctg cgtcatgtgg
     2041 atgatcgtgg aatcaccctg gaaccgggcg agatcattct gcgctttttc cgaaaggtaa
     2101 tcggccatcg tatcaccttc ctgcatgtgt gtaagcgatt tccatactgc ttcattcgac
     2161 gtgatcgtca gctcgcggta tttgttgtta taggaaacga ggaatgaatc gacgcgcttt
     2221 acaaatgtgg cgtcgtcttc cgccgggttg ttcttacagc tcaacggaag aaaaaaaaca
     2281 gcagcgagcg gcagaaggaa aagaatgcgt ttgtttttca tgaaaaaagt aaatcaggtt
     2341 ggaatgccaa atttagaaga caatcccgaa tgaaagacat cacctgtaca tctatctttc
     2401 agcctgtaa
//
LOCUS       DASTSF010000307         3231 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_943716,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000307 DASTSF010000000
VERSION     DASTSF010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3231
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_943716"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            295..564
                     /gene="rpsO"
                     /locus_tag="VFU15_08815"
     CDS             295..564
                     /gene="rpsO"
                     /locus_tag="VFU15_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009414989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEU4717924.1"
                     /translation="MYLTSDIKKDIFKKFGKSEKDTGSPEGQIALFTHRINHLTGHLK
                     QKPKDKSSERSLVLLVGKRKSLLDYLRKNDIERYRAILKKLDIRK"
     gene            673..2910
                     /gene="pnp"
                     /locus_tag="VFU15_08820"
     CDS             673..2910
                     /gene="pnp"
                     /locus_tag="VFU15_08820"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009217747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEU4717925.1"
                     /translation="MKLGIQKTFDIGDGRTITIETGKLAKQADGSVVVRFGNTMLLAT
                     VVSAPEAKAGVDFMPLTVDYREMFAAAGRIPGSFFKREAKPSDLEILVSRLVDRALRP
                     LFPDDYHAETQVLIYLISSDKTILPDCMAGLAASAALAVSDVPFNGPISEVRVARIDG
                     KFMINPTIEQMKLSDIDMIVAANENDIVMVEGEMKEISEADMLGAIKFAHEAIKLQIA
                     AIRELAAMVEKSKTKREYSHETHKEELREKVKASTYDKALAVAKKGNPNKHERSDAFK
                     AVFEEFWNSLPEEEQTDENKSLAKTYYHDVEKEAVRRMILDDRTRLDGRGLTNIRPIW
                     CEVDYLPMAHGSAVFTRGETQSLTTVTLGTKLDQQMIDTTVIQGDVNFLLHYNFPPFS
                     TGEAKPMRGTGRREIGHGNLALRALKNVLPDDNPYTIRIVSDILESNGSSSMATVCAG
                     TLALLDCGVKIKKPVSGIAMGLITDDSGKYAVLSDILGDEDHLGDMDFKVCGTRDGIT
                     AVQMDLKVHGLRYEVMAEALEQARQGREHILGEMMKTISAPREDFKNHAPRLVQLTIP
                     REYIGAVIGPGGKIIQEIQRETGTTIVIEEKGEQGIVDIVSENKDSIDRAVARIKGIT
                     AVPEVGEVYEGKVKSIMPFGAFVEFLPGKDGLLHISEIEWRRLETMEGVLKEGDVIQV
                     KLVEVDPKTGKFRLSRKVLLPKPEGFQERPPRQQGEKRHHDKPKQHHDKPHHDAPHHD
                     NNGNQ"
     gene            2982..>3231
                     /locus_tag="VFU15_08825"
     CDS             2982..>3231
                     /locus_tag="VFU15_08825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717926.1"
                     /translation="MKPAFRFSVAILLIFSSSCHHEKPQPRNGAYYCADQEWEIMWRD
                     SLELFMLDASGQEISSLDNMKDAVAHMEAGMDPVPPALY"
BASE COUNT          840 a          918 c          838 g          635 t
ORIGIN      
        1 gagaacattt cgctcaaatt cccgatcccg ctggctttcg aatcaacagt gggtatccgc
       61 tattatttca ccgataacat aggcgcctac ctggaagccg ggtggggaag ttcgctgttg
      121 cagggcggac tggccgtaaa attctgatgc aggaaagccc tccgcggagg gcttttttct
      181 tatttcctgg tctggcgatg ccgcctggtt ttgcagatcg ggcagaattc gtatctttgc
      241 cgtcccatta ggggaaacaa ctcactgatt cttaatccac aaacagcaaa aatcatgtac
      301 ctgacgtcgg acattaagaa ggacatcttc aaaaaattcg gaaaatcgga aaaagacacc
      361 ggttcacccg aaggacagat cgcccttttc acgcatcgca tcaatcacct caccgggcac
      421 ctcaagcaga agccgaaaga taaaagttcg gagcgctcgc tggtcctcct tgtcggcaaa
      481 cgcaaaagcc tgctggatta cctcaggaaa aacgatatcg aacggtaccg ggctatcctg
      541 aaaaagctcg acatccgtaa ataaatgcaa gaccatttca atacaggaag gcatcccttt
      601 aagtcagcag ggatgctttc tcatttttag cgacccgctg acaagctgca acaaacaaag
      661 agaaaaacaa ttatgaaact aggaattcaa aaaactttcg acatcggcga tggacgtacc
      721 atcactattg aaaccgggaa gcttgccaag caggcagacg gttccgtcgt tgtccgtttc
      781 ggaaacacca tgctgctggc caccgttgta tcggcgccgg aagcaaaagc gggagtcgac
      841 ttcatgccgc tcaccgttga ttaccgcgaa atgttcgctg ccgccgggcg catcccgggc
      901 agctttttca aacgcgaagc aaaaccgtcc gatcttgaaa ttctcgtgag ccgcctcgtc
      961 gaccgggcgc ttcgcccgct tttcccggat gactaccacg ccgagacaca ggtgctcatt
     1021 taccttatct cttccgacaa gaccatcctt cccgactgca tggcaggact cgcggcatct
     1081 gctgcgctcg cggtttccga tgtgccgttc aacggcccca tcagcgaagt gcgcgtggca
     1141 aggatcgacg ggaagttcat gatcaacccg accatcgagc agatgaagct ttccgacatc
     1201 gacatgatcg tcgctgccaa tgaaaacgac atcgtgatgg tggaaggtga aatgaaggaa
     1261 atttccgaag ccgacatgct cggcgccatc aagttcgcgc acgaggcgat caagctccag
     1321 atcgctgcga tcagggaact ggccgcgatg gtggaaaaat ccaaaaccaa acgcgaatac
     1381 agccacgaaa cgcacaaaga agagctgcgt gaaaaagtga aagcttccac gtacgacaaa
     1441 gctctcgccg ttgcgaaaaa aggaaacccg aacaagcacg agcgctccga tgcgttcaaa
     1501 gcggtgttcg aagaattctg gaactcgctg ccggaagaag agcagaccga tgagaacaag
     1561 tcgctggcga aaacctatta ccacgacgtc gaaaaagaag cggtccgccg catgatcctc
     1621 gacgaccgta cccgcctcga cgggcgcggg ctgacgaaca tccgtcccat ctggtgcgag
     1681 gtcgattatc ttcccatggc gcacggttcc gccgtcttca cacgcggtga aacgcaatcg
     1741 cttaccactg ttacgctcgg cacgaagctc gatcagcaga tgatcgatac gaccgtgatc
     1801 cagggcgacg tgaatttcct tctccattac aactttcccc cgttctccac cggcgaagca
     1861 aagcctatgc gcggaacagg acgccgcgag atcggccacg gaaacctcgc gctgcgtgcg
     1921 ctgaagaacg tgctcccgga tgataatccg tacacgatcc gtatcgtttc cgacatcctc
     1981 gaatcgaacg gttcttcttc catggccacg gtctgcgcag gaacgcttgc gctgctcgac
     2041 tgcggcgtga agatcaaaaa accggtgtcc ggcatcgcaa tgggactgat cacggatgat
     2101 tccggcaaat acgccgtgct ttccgacatt ctcggcgatg aagaccacct cggtgatatg
     2161 gatttcaaag tgtgcggaac acgcgacggg atcaccgcag ttcagatgga cctgaaagtg
     2221 cacggacttc gctatgaagt gatggccgaa gcgctcgaac aggcccgcca gggacgcgag
     2281 cacatcctcg gagaaatgat gaaaaccatt tccgcgccgc gtgaagattt caaaaaccac
     2341 gcaccgcgtc tcgtgcagct caccatcccg cgcgaataca tcggcgccgt tatcgggccc
     2401 ggcggaaaga ttatccagga gatccagcgc gaaaccggca cgaccatcgt catcgaagaa
     2461 aaaggcgaac agggcatcgt cgacatcgtt tccgagaaca aggattccat cgatcgtgca
     2521 gtggcgcgca tcaagggaat caccgcggtc ccggaagtgg gcgaagtgta cgaaggcaaa
     2581 gtgaaaagca tcatgccttt cggcgcgttc gtggaattcc ttcccggcaa agacggcctg
     2641 ctgcacattt cggagatcga atggcgcagg cttgaaacca tggaaggcgt gctcaaagaa
     2701 ggcgatgtca tccaggtgaa gctggtggaa gttgatccca aaacgggcaa attccgcctc
     2761 tcgcgcaaag tgcttcttcc gaaaccggaa ggcttccagg aacgtcctcc ccgccagcag
     2821 ggcgagaaac gtcaccacga caagccgaag cagcaccacg acaaaccgca tcatgatgcg
     2881 ccgcatcacg ataacaacgg aaaccagtaa taatttccgg tgatcatgaa aatcccgttc
     2941 agcaatgagc gggattttta gttttgcata accccgttcc gatgaaacct gctttccgat
     3001 tctccgttgc gatcctgctg atcttttctt cttcctgcca tcacgaaaag ccgcaaccgc
     3061 gcaatggcgc ttattactgc gccgaccagg aatgggaaat tatgtggcgg gacagcctcg
     3121 agcttttcat gctggatgct tccgggcagg aaatctcctc ccttgataac atgaaagatg
     3181 ccgtcgccca catggaggca ggcatggacc cggttccgcc ggccctgtat g
//
LOCUS       DASTSF010000308         5229 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1211073,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000308 DASTSF010000000
VERSION     DASTSF010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5229
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1211073"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1059
                     /locus_tag="VFU15_08830"
     CDS             <1..1059
                     /locus_tag="VFU15_08830"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEU4717927.1"
                     /translation="GIVSDNSFTSVQFTDIVVDNNGYVHVSFFGSKDSVNYAVWHSVS
                     TDGGMTFSTPVKISDAHVPGFSGDQPNANIVGIDPTRFYPCQHLACDNSSGPNANNLY
                     ATWTANGTTSAATAGLDIYFSRSTDGGATWSSPVIINNDTATAADNFYSAITMNGNGV
                     VLLSWYDRRTDPVNNKTTHYYFAYSSDGGQTFQGNQQITNTPTDFSTIGAQNNGFGIG
                     EYTQIVATGAYAIPVWTDGRTNDGDCNIYCATIQWATFSGVAEPVAVNNHHISSVYPN
                     PSSGSLSITLDFGTAQNANVTITDLSGRTVMNVCDGKTFEGKSRMDIDVSALAAGNYL
                     LVLRTENGKETSKFTVAR"
     gene            complement(1072..1581)
                     /locus_tag="VFU15_08835"
     CDS             complement(1072..1581)
                     /locus_tag="VFU15_08835"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF025034.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="retropepsin-like aspartic protease"
                     /protein_id="HEU4717928.1"
                     /translation="MKVHIPIRLLRIEGDGFHLLAKIRINGKNADVILDTGASKTVFD
                     KTQVLAFVKEEKLNENERLSTGLGTASMQSHTLVIRKIEMGKIILENYNSVILDLSHV
                     NQTYSALGLKQIVGVIGSDLFAEFKALIDYGKKELLLTVTPQKKAKKKAAPKRPVKKK
                     KKPAPKKKK"
     gene            complement(1647..2585)
                     /gene="mdh"
                     /locus_tag="VFU15_08840"
     CDS             complement(1647..2585)
                     /gene="mdh"
                     /locus_tag="VFU15_08840"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HEU4717929.1"
                     /translation="MKVTVVGAGNVGATCADVIAHKELANEVILLDIKPNFAEGKALD
                     IWETSPINLYDTRVKGSTEDYSKTANSDVVVITSGLPRKPGMSRDDLISTNAAIVKTV
                     TENVIRHSPNAIIIIVSNPLDVMTYCAYLTAKVDSSRVFGMAGILDTARYRAFLAEAL
                     NCSPKDIQAVLMGGHGDTMVPLPRYTTVGGIPVTELIGKAELDAIVERTKKGGGEIVN
                     LLGTSAWYAPGAAAAQMVEAVLRDQKRIFPVCAWLNGEYGLKNVYLGVPVKLGRKGIE
                     QIIELKLNADEMALVNDSAKAVKEVMSVLDGMNIMA"
     gene            2738..5101
                     /locus_tag="VFU15_08845"
     CDS             2738..5101
                     /locus_tag="VFU15_08845"
                     /inference="COORDINATES: protein motif:HMM:NF040138.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HEU4717930.1"
                     /translation="MKLPHPFASLLITLLLAAGSLHAQTPNNQDCLDAIPICQNVYST
                     TNSYSGEGNIQNEINNSSSCLGSGELNDVWYTFTVQQSGNLNFLITPNNSADDYDWAV
                     YNLTNNNCSDIFSNPALEVSCNFSGTSGSTGPNGGSSFSSQNAGGTPYNAVVPVVAGQ
                     TYVVNVSNYSSTQNGYTIDFGASTATIFDQTPPQILSVTNPGCGGNTLTVTFSENILC
                     NTVQASDFSFTGPGGPFTVTAVNSPACAQGATYDNTYTLTISPAISGSGSFTANLVGP
                     VTDLCGNIAIYPASLPFTVGSFSYTNASTPTSCGGNNGTATVTPNGTGPFTYVWSPNV
                     SSTNSANNLAAGVYTVTITDQSTGCSAQDTIHVNSNNSLSATTSGDDTICPGQQATMT
                     ANVIGGNPPVTYTWSNGLPNQASVTVSPAATTTYTLNITDGTGCTAGPLLFTITVAGP
                     LSVTAAGTSPVCSGGGTVLNANASGGNGPYSYVWTPGNINGSSANVSPVATTDYTVTA
                     TDACGQTATTDFNVVVQPVPVISFTADTTYGCSPLSVHFTIDTTGFGNATFLWDFDDN
                     GATSTAAEPSYTFTSAGCHNVSLTVTVPPGCSVTQTYPCMIKTFAQPVALITATPSFT
                     DILNPVVTFDNQSTGATSWYWSYGDSTSSTVFEENHTYDFPGVYPVYLIAQNDSGCSD
                     TAYVTITVNDFHTFYVPNAFTPNGNGNNEVFRPEFTNILSDGYSMMIFDRWGNLVFET
                     SDPNVGWNGCWKNTGKMCQLDEYVYKIRYTDNMHQQYLLIGGVSLIR"
BASE COUNT         1202 a         1469 c         1295 g         1263 t
ORIGIN      
        1 ggaattgttt ccgacaatag cttcacaagc gttcagttca ccgatatcgt tgttgacaac
       61 aacggttacg tgcacgtttc atttttcgga tcgaaggaca gcgtgaatta cgctgtatgg
      121 cattctgttt ccacggatgg aggaatgaca ttcagcacac cggtgaaaat ttccgatgcg
      181 catgtcccgg gcttcagcgg cgaccagccg aatgcgaata tcgtcggtat agatcccacg
      241 cgtttttatc cctgccagca tcttgcctgc gacaactctt ccggtccgaa tgcaaataat
      301 ctttacgcaa catggactgc gaacgggacc acttccgcag caacggcggg acttgatatt
      361 tatttttcac gctccacgga tggaggcgcg acctggtcct caccggtgat catcaataac
      421 gacacggcaa ccgctgcgga taatttttat tcggcgataa cgatgaacgg gaacggcgtc
      481 gtcctgcttt cgtggtatga ccgccgcacg gatcctgtca acaacaaaac gacgcactat
      541 tattttgcgt attcgtccga cggcggacaa acgttccagg gcaaccagca gatcacgaat
      601 acacccactg atttttcaac gatcggtgcg cagaacaacg gattcggcat cggcgaatac
      661 acacagatcg tcgcaacggg cgcatacgcg attcccgtgt ggacggacgg ccgcaccaac
      721 gacggcgact gcaacattta ctgcgcaacc atccagtggg cgacgttcag cggagtggcg
      781 gaacctgtgg cggtgaacaa ccaccacatt tcttccgttt atcctaaccc gtcgtccggc
      841 tcgctcagca taacactcga tttcggaaca gcgcaaaacg cgaacgtgac gatcaccgat
      901 ctcagcggaa gaaccgtgat gaacgtgtgc gatggaaaaa cattcgaagg aaaaagcagg
      961 atggacatcg acgtgagcgc gctcgctgcc ggtaattacc tgctcgtgct gagaacggaa
     1021 aacgggaaag agacttcgaa atttactgtg gcgcggtaac gctgcacagg atcatttttt
     1081 ctttttcggc gccggctttt tctttttctt caccgggcgt ttcggcgcgg ctttcttctt
     1141 cgcttttttc tgcggagtca ccgtgagcag aagctctttt ttcccgtaat cgatcagcgc
     1201 tttgaattct gcaaaaagat cgctgccgat caccccgacg atctgtttca gcccgagcgc
     1261 ggaatacgtc tggttgacgt gactgagatc gaggatcacg ctgttgtaat tttccaggat
     1321 gatctttccc atttcgatct tccggatcac cagcgtgtgg ctttgcatgg aagcagttcc
     1381 cagcccggtg gaaagacgtt cgttctcgtt cagcttttcc tctttcacga aagcgagcac
     1441 ctgtgtttta tcgaaaacgg ttttcgacgc gccggtatcc aggatgacat ctgcgttttt
     1501 tccgttgatc ctgattttcg cgaggagatg gaatccgtcg ccttcaatgc gcagcaacct
     1561 gatcgggatg tgtactttca tcttttcctt tttaaaaaag aaggttggaa atgatccaac
     1621 cttcttccgt ttttttcgtg aactaattac gccatgatgt tcattccgtc gaggacgctc
     1681 atcacttcct ttaccgcttt tgcagagtcg ttgacaagcg ccatctcatc tgcgttcagc
     1741 ttgagctcga tgatctgctc gatgcctttt cttccgagct tcacgggaac gccgaggtaa
     1801 acattcttca gcccgtattc accgttgagc catgcgcaga cggggaaaat gcgtttctgg
     1861 tcgcgcagca cggcttcgac catttgcgct gccgcagcac cgggcgcata ccatgcggaa
     1921 gtgccgagca ggttgacgat ctcgccgccg ccttttttcg tgcgttcgac gatcgcgtcg
     1981 agctccgcct tgccgatgag ctccgttacg ggaattccgc cgacagtagt ataacgcggc
     2041 agcggcacca tcgtgtcgcc atgcccgccc atgagcaccg cctggatatc tttcggtgag
     2101 cagttgagcg cttccgcgag gaaagcgcgg taacgtgcgg tgtcgagaat tccggccatg
     2161 ccgaacacgc ggctggaatc gaccttcgcg gtgagatacg cgcagtacgt catcacgtcg
     2221 agcggatttg acacgatgat gatgatggcg ttgggcgaat gcctgatcac attttcagtt
     2281 accgttttta cgatcgcagc gttcgtggaa ataagatcat cacggctcat gccgggtttg
     2341 cggggcagac cggaagtgat caccaccacg tcggagttcg ctgttttgct gtaatcttcc
     2401 gtcgagcctt tcacgcgtgt gtcgtaaaga ttgatcggcg aagtttccca gatatcgagc
     2461 gctttccctt ctgcgaaatt cggcttgatg tccagcagaa tgacctcgtt ggcgagttct
     2521 ttgtgtgcaa tcacatctgc acaggtggcg cccacgtttc cggcacctac tacggtaact
     2581 ttcatttgcg ttgtttttat gcggttgtaa attgagttga cgttcagaac ggtaaaatta
     2641 gaaatagtat gacttcacac ttccctgtgg catgattttt tcggccacct gtcaaactaa
     2701 taccgtgtca tcgctattta gtctaatttt gcgcagcatg aaattacccc atcccttcgc
     2761 ctccttgctc atcaccctgt tgcttgcagc gggatcgctt catgcgcaga cacccaacaa
     2821 ccaggattgc ctcgatgcga tcccgatctg ccagaacgtt tattcgacaa ccaattccta
     2881 cagcggcgaa gggaatatcc agaacgagat caacaattct tcctcctgtc ttggcagcgg
     2941 cgagctgaat gacgtctggt atacgttcac cgtgcagcaa agcggcaacc tgaacttcct
     3001 gatcacaccg aacaacagcg cggacgacta cgactgggcc gtgtacaacc tcacgaacaa
     3061 caactgctcg gatatttttt ccaatcctgc gctcgaggtg agctgcaatt tttcgggaac
     3121 atcgggatcg accgggccga acggcggttc ttctttctcc tcgcagaatg ccggcggaac
     3181 accttacaat gccgtcgtgc cggttgtcgc cgggcaaacg tacgtggtga acgtgagcaa
     3241 ttattcctcc acacagaatg ggtacacgat cgatttcggc gcatccactg cgaccatctt
     3301 cgaccagacg ccgccgcaga tactcagcgt tacaaacccg ggctgcggag ggaacacgct
     3361 gaccgtgaca ttctcggaaa atatcctgtg caatacagtg caggcaagcg atttttcctt
     3421 caccgggccc gggggaccgt tcactgtcac ggccgtcaac tctcccgcgt gcgcacaggg
     3481 cgcgacgtac gacaacacct acacgctaac gatctcgccg gcgatctccg gttcgggatc
     3541 gttcacggca aatcttgtcg gaccggtaac agacctttgc ggaaacatcg cgatttaccc
     3601 ggcgtcgctt cctttcaccg tcggatcatt ttcatatacc aacgcatcca caccgacaag
     3661 ctgtggagga aacaacggaa cggcaaccgt tacacctaac gggaccggac ctttcacgta
     3721 tgtgtggagt ccgaacgtaa gcagcacgaa ttccgcgaac aatctcgctg ccggagttta
     3781 caccgtgacg atcacggacc agtcgaccgg ctgcagcgcg caggatacga tacacgtaaa
     3841 ttccaacaat tcactttccg ccaccacttc aggtgatgac acgatctgcc cgggacaaca
     3901 ggctacaatg acggcgaatg tcatcggtgg aaatcctccc gtgacctaca catggagcaa
     3961 cgggttaccg aaccaggcaa gcgttactgt ttcgcctgct gcaacaacca cgtatacgtt
     4021 gaacataaca gacggaacgg gctgcacggc cggaccgctg ctattcacaa ttacagttgc
     4081 cggaccgctt tctgtgactg cagcaggaac aagtcccgtc tgctcgggcg gaggaaccgt
     4141 gctgaatgca aatgcgtccg gcgggaacgg tccttattcc tatgtgtgga cacccggaaa
     4201 tatcaacgga tcttccgcga acgtttctcc ggtcgccacg accgactaca ccgtaaccgc
     4261 aacggacgcc tgcggacaaa cagcgaccac cgatttcaat gtcgttgttc agcctgtgcc
     4321 ggttatttca ttcacggctg acaccacata cggatgttcc ccgctgtccg ttcatttcac
     4381 gatcgacacc accggtttcg gaaacgcaac attcctctgg gattttgacg acaacggcgc
     4441 cacgtcgact gccgcagaac catcgtacac atttacttca gccggctgcc acaacgtgag
     4501 cctgaccgtt actgttccgc ccggatgttc cgttacgcag acatatccgt gcatgatcaa
     4561 aacatttgcg cagcccgttg cgctgatcac tgcaacgcct tccttcacgg atatcctcaa
     4621 cccggtggtg acattcgaca accagtcgac cggcgcaact tcctggtact ggtcgtacgg
     4681 cgacagcaca tcatccactg tgttcgaaga aaaccacacg tacgatttcc ccggcgttta
     4741 cccggtttat ctcattgcgc aaaacgattc cggctgttcg gacaccgctt acgtaaccat
     4801 caccgtaaat gattttcata cgttctacgt gcccaatgcg ttcacaccga acggcaacgg
     4861 caacaatgaa gtcttcaggc cggagttcac caatatcctg agcgatggtt attccatgat
     4921 gatcttcgac cgctggggaa atcttgtttt tgaaacgtca gacccgaacg tgggctggaa
     4981 cggctgctgg aaaaataccg gcaagatgtg ccagctggat gaatacgttt acaagatcag
     5041 gtacacggat aacatgcatc agcagtacct tctcatcggt ggcgtaagcc ttatccggta
     5101 attgttcctc ctgttttcat ttcagtttca acaaaagacg cccggaatac cgtggtttat
     5161 ctataataaa caggccttaa gaatggaata tttatttttt gtcgaaggaa tagtattgtt
     5221 ggtctaatt
//
LOCUS       DASTSF010000309         4510 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_4842,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000309 DASTSF010000000
VERSION     DASTSF010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4510
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4842"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            129..1238
                     /locus_tag="VFU15_08850"
     CDS             129..1238
                     /locus_tag="VFU15_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019944462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU4717931.1"
                     /translation="MKQFLAFVRKEFLHIIRDGRTLLILFGIPVAQVTLFGYVLSNDI
                     KDVPVAVLDNSRDDASTTLLSRISSSGYFHIDDYLKTENEIDAAFRQGKIKMAFVIPG
                     NFSGKLYHGQKAEVEIVADATDINMASTLTSYAQSIIRSYETEINPGVQPDMQIVPQV
                     RMTFNPELKSVYMFIPGVISIVMILISAMLTSITIAREKEMGTMEILMISPLKPLQII
                     IGKVLPYLVLSIINCTIILLMGFFIFGMPMNGNFLTLGLECILYITLALSLGILISSV
                     ANTQQTAMFASMLALMMPTMLLSGFVFPVENMPVVLQGLSYIMPAKYFIIILKGVMIK
                     GSGIKMLWQPTLIMLGFTLFFLFVSLRRIKNRLTA"
     gene            1235..2353
                     /locus_tag="VFU15_08855"
     CDS             1235..2353
                     /locus_tag="VFU15_08855"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU4717932.1"
                     /translation="MKNILHIIRKEIIQVRRNKQMLPMLTILPVVQVIVLSFAANFEI
                     RHLQLAVVDNDHSVESRQLVQSFTSSGFFELAGYATNAKDAFEMMQRDQADIVLEFPA
                     HFGRDLTRLNYAPVSVRLNAVNSQKAGVSAAYALTIIGDYNERIRAETGSTLQPQSYY
                     TLAAAVSNWYNPLLNYKTLMVPGILAVMVSLLIMIVSAMNIVREKEIGTIEQLNVTPV
                     KKYQFILGKLIPFWVMGHIIFWVGITAGILIFHVPFVGNPLVLELFLAVYLPVMLGIG
                     LFISTLVDTQQQAMFVAFFFVLVFILMSGFFTPVENMPAWAQKVDVINPMKYLVAVNR
                     LVLLKGAGLKEISPFLFFILGYGITVNVLAVFRYRKTV"
     gene            2455..3033
                     /locus_tag="VFU15_08860"
     CDS             2455..3033
                     /locus_tag="VFU15_08860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717933.1"
                     /translation="MFARNSRLFVASMAFIGIVAFSCKGKVEYQTVTVKDYSVDIPKH
                     LVVATDLNDDASLQYQDPIREFYVIVIDESKDDFINTFKEIGEYDSTKTAEKNYREVQ
                     LKSMKDHITMKSDPVLKAAKINGLDAEIADFTGNVEGIQSDIWYKVAFIEGPDNMYML
                     MEWTKDSDKDKNSDEFDGVINSFKVNKTAAAK"
     gene            3237..4016
                     /locus_tag="VFU15_08865"
     CDS             3237..4016
                     /locus_tag="VFU15_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEU4717934.1"
                     /translation="MEFQNLLVNLEEGILTITINRPDKLNALNKTTLSELRSAMGEAK
                     KSADVKGIIITGSGPKAFVAGADISEFAGLGPKEGADFAENGQEIFRSIELFPKPVIA
                     AVNGFALGGGCELAMACHMRIAAENAKFGQPEVSLGLIPGYGGTQRLTMHIGRGRATE
                     LLMTGDIVDAKDAYRVGLVNYLVPNDQLIPKCREILLKIFTKAPFAVSEIVKCVDAHY
                     KDGVDGYAFEVESFGRCCGTEDFKEGSSAFMEKRKAAFKGK"
     gene            complement(4089..>4510)
                     /locus_tag="VFU15_08870"
     CDS             complement(4089..>4510)
                     /locus_tag="VFU15_08870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717935.1"
                     /translation="DQSNSKGDKSLTGSWEEKGSDDYEWKVTLDDNMYRIEKTNTKDG
                     GDPTVYKGYLSDVGGSTWMNVYELDDYSSSSDRKYYIYKVEKKGDDADRVKMRAMTDN
                     VTEEFETSADLKAFIKKYMDLSFFYNKDDEKTFYKND"
BASE COUNT         1060 a         1231 c         1185 g         1034 t
ORIGIN      
        1 acgaggcgga aacaatggat gaagtgttcc ggcacctggc gcgcggaaca tcgaccaaat
       61 aaaaatgaca ggcgccaaat gaaaaaagac cggacaataa acaccgctta tactttttca
      121 tcccggaaat gaaacagttc ctcgcattcg tacgcaaaga gttcctgcac atcatccgtg
      181 acgggcggac gctgctgatc ctgttcggca ttcccgtcgc gcaggtgacg ctcttcgggt
      241 acgtactcag caatgacatc aaagacgttc ccgtggccgt gctcgacaat tcccgcgatg
      301 acgcgagcac gacgctgctc agccgcattt cctcttcggg ttatttccac atcgacgact
      361 acctgaaaac cgagaacgag atcgatgccg cattccggca ggggaaaata aaaatggcgt
      421 tcgtcatccc cgggaatttc agcgggaagc tgtatcacgg acaaaaagca gaagtggaga
      481 tcgtcgccga cgccaccgac atcaacatgg ccagcacgct cacgagctat gcgcagtcga
      541 tcatccgttc gtacgaaaca gagatcaacc ctggcgtgca gccggacatg cagatcgttc
      601 cgcaggtgcg catgacgttc aatcccgagc tgaaatccgt ttacatgttc attcccggcg
      661 tgatctcgat cgtgatgatc ctgatctcgg cgatgcttac gtccatcacc atcgcacgcg
      721 aaaaagaaat gggcacgatg gagatcctga tgatctcccc gctgaagccg ctgcagatca
      781 tcatcgggaa agtgcttccg tatctcgtgc tttccatcat caactgcacg atcatcctgc
      841 tgatgggatt tttcattttc gggatgccga tgaacgggaa tttcctcacg ctgggcctcg
      901 agtgcattct ctacatcacg ctggcgcttt cgctgggcat tctcatttcg tcggtcgcca
      961 atacgcagca gacggcgatg ttcgcttcga tgctcgcgct gatgatgccg acgatgctgc
     1021 tttccgggtt tgttttcccg gtggagaaca tgccggtggt gctgcaggga ttgtcgtaca
     1081 tcatgcccgc aaaatatttc atcatcattc tcaagggcgt gatgatcaaa ggcagcggaa
     1141 taaagatgct ctggcagccc acgctgatca tgctgggctt cacgctcttt ttcctgttcg
     1201 tcagcctgcg ccgcatcaaa aaccgcctga ccgcatgaag aacatcctgc acatcatacg
     1261 caaggagatc atccaggtgc ggcgcaacaa gcagatgctg cccatgctga cgatcctccc
     1321 cgtcgtgcag gtgatcgtgc tttcgttcgc ggcgaatttc gagatccgcc acctgcagct
     1381 ggcggtggtg gacaacgacc acagcgtgga atcgcggcag ctggtgcagt cgttcacctc
     1441 ctcgggattt ttcgagctgg ccggctacgc aaccaatgcg aaagatgcgt tcgaaatgat
     1501 gcagcgcgac caggccgaca tcgtgctgga atttccggcg cacttcggac gcgacctcac
     1561 gcggctgaac tacgcgcctg tctccgtgcg cctcaatgcc gtgaacagcc agaaggccgg
     1621 cgtgagcgcc gcgtacgcac tgacgatcat cggcgattac aacgagcgca tccgcgcgga
     1681 aacgggaagc acgctgcagc cgcagagcta ttacacgctc gcggccgccg tttccaactg
     1741 gtacaacccg ctgctgaatt acaaaacgct catggtgccg ggcattctcg ccgtgatggt
     1801 ttccctgctg atcatgatcg tttcggcaat gaacatcgtg cgtgaaaaag agatcggcac
     1861 gatcgagcag ctgaacgtaa cgccggtgaa aaaataccag ttcattctcg gcaagctcat
     1921 cccgttctgg gtgatgggac acatcatttt ctgggtcggc ataacggcgg gcattctcat
     1981 tttccatgtt ccgttcgtcg ggaacccgct ggtgctggaa ctgttcctgg ccgtgtacct
     2041 tcccgtgatg ctcggcatcg ggctgttcat ttccacgctg gtcgatacgc agcagcaggc
     2101 gatgttcgtg gcgttctttt tcgtgcttgt tttcattctc atgagcgggt ttttcacgcc
     2161 agtcgagaac atgcccgcct gggcgcagaa agtggatgtg atcaacccga tgaaatacct
     2221 cgttgccgtg aaccgcctcg tgctgctgaa aggcgcgggg ctgaaagaga tcagtccttt
     2281 cctctttttt atccttggtt acggcattac cgtcaacgtg ctcgcggttt tccggtaccg
     2341 taaaacggta taataggcgg tttccgcgct gaatttcagt ggcggctttg gcaaacgtcc
     2401 tatttgcgct accttgcgcc cgaaacaaat ctgtacacac caaaaccatc cactatgttc
     2461 gcccggaact cccgactttt cgttgcttcg atggcattca tcggcatcgt tgcattttcc
     2521 tgcaagggga aagtagaata ccagactgtc accgtgaaag attattccgt tgacattccg
     2581 aaacacctcg ttgtggccac cgatctgaat gacgacgcgt cgctgcagta ccaggatccc
     2641 atccgtgaat tctacgtgat cgtgatcgat gaatcgaaag atgattttat caatacgttc
     2701 aaggagatcg gcgaatacga ctccacgaaa acggctgaaa aaaattaccg tgaggtgcag
     2761 ctgaaatcca tgaaagatca catcacgatg aaatcggacc ccgtcctgaa agccgcgaag
     2821 atcaacggcc tcgatgccga gatcgctgat ttcaccggca acgtggaagg catccagtcc
     2881 gacatctggt acaaagtcgc cttcatcgaa ggacccgaca acatgtacat gctgatggaa
     2941 tggacgaaag attccgacaa agacaagaac agcgacgagt tcgatggcgt gatcaattca
     3001 ttcaaagtga acaaaaccgc agcggcgaaa taaccgctct cgtttgcaca agacagggcc
     3061 ccggttattc cggggctttt cttttgcgtt tttttgggca aaacttttta aaactttgac
     3121 acgtacgtct gcatccgcac aaagaattcc tcctgcggat gattaatttt accggtcaca
     3181 aaattcaagc aatgaatcaa tcacaaaccg acgataccgt cgcaacagtt tctgaaatgg
     3241 aatttcaaaa cctcctggtc aatctcgaag agggcatact gacgatcacc atcaaccgcc
     3301 ccgacaagct caatgcgctc aacaaaacca cgttgtctga gctgcgctcg gcaatgggcg
     3361 aagcgaaaaa atcggcggac gtaaaaggca tcatcatcac cggctcggga ccgaaagcgt
     3421 tcgtggccgg tgcagacatt tccgagttcg caggactcgg tccgaaagaa ggcgccgact
     3481 ttgcagagaa cgggcaggag atcttccgca gcatcgagct gttcccgaaa ccggtgatcg
     3541 cggcggtcaa cgggtttgcg ctcggcggcg gatgcgagct ggccatggcg tgccacatgc
     3601 gcattgcggc agagaacgcg aagttcggtc agccggaagt gagcctcggt cttatcccgg
     3661 gctacggcgg cacgcagcgt ctcaccatgc acatcgggcg cggaagagcg acggagctgc
     3721 tcatgaccgg cgacatcgtg gacgccaaag acgcgtaccg tgtcgggctt gtcaactatc
     3781 tcgttccgaa cgatcagctt attcccaaat gccgcgagat cctgctcaag atattcacca
     3841 aggcgccgtt cgccgtttcg gagatcgtga aatgcgtgga tgcgcattac aaggacggtg
     3901 tggacggcta cgcattcgaa gtggaatcct tcgggcgctg ctgcgggacg gaagatttca
     3961 aggaaggttc ttccgcgttc atggaaaaac ggaaagcggc tttcaaagga aaataaagac
     4021 gcggatcagt tcaataaaaa aacccgggcg ggaagtcgtc cgggtttttt gttttccgaa
     4081 aagaatgatc agtcgttctt gtagaacgtc ttctcgtcgt ccttgttgta gaagaaggaa
     4141 agatccatgt acttcttgat gaacgctttc aggtcggcag acgtttcgaa ttcctccgtt
     4201 acgttgtcgg tcattgcgcg catcttcaca cggtcggcgt catcgccttt cttctccact
     4261 ttgtagatgt aatacttgcg gtcggaagag ctgctgtaat catccagctc atagacgttc
     4321 atccaggtag agccgcctac atcggaaagg tatcctttgt aaacggtggg atcgcctccg
     4381 tccttcgtgt tggttttctc gatacggtac atgttgtcat cgagggtgac tttccactcg
     4441 taatcatcgg aacctttctc ttcccagctt ccggtaagag acttgtcgcc ttttgaatta
     4501 gactggtcgt
//
LOCUS       DASTSF010000310         5298 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_18813,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000310 DASTSF010000000
VERSION     DASTSF010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5298
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18813"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..449
                     /locus_tag="VFU15_08875"
     CDS             <1..449
                     /locus_tag="VFU15_08875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717936.1"
                     /translation="VPPALYGIRPEKETRTCTIMGVPVSVNETIRPGRILFNAITLHP
                     GDTSETRWFVHRELAEAFCQDHDLDTLLSWKHCRLGRGTDRFDGRTFYSVSVQSPQED
                     FPRITDYFGCARNIFYHIHVEAPSKKTWDAFAALLHTLRFVEFERN"
     gene            616..1485
                     /locus_tag="VFU15_08880"
     CDS             616..1485
                     /locus_tag="VFU15_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015482077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU4717937.1"
                     /translation="MRQLKISKQVTNRETASLDKYLQEIGRVDLITAEEEVDLARKIK
                     QGDQYALAKMVNANLRFVVSVSKQYQNQGLSLPDLINEGNLGLIKAAQRFDETRGFKF
                     ISYAVWWIRQSILQALAEQSRIVRLPLNKIGSINKINKTFAKLEQEFEREPNADEIAA
                     ALELSPEDIREALRNTGRHVSMDAPLASGDENTSNMYDVMQSDDTPSPENTLINESLR
                     KEIERALSTLTTREADVVRLYFGLNGEHAMTLEEIGERFDLTRERVRQIKEKAIRRLK
                     HTSRSKLLKTYLG"
     gene            complement(1562..3619)
                     /locus_tag="VFU15_08885"
     CDS             complement(1562..3619)
                     /locus_tag="VFU15_08885"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEU4717938.1"
                     /translation="MLLLVFVPFRGVSQEPDTNKINEVFAKIWPMHYRNMDSAMMLSG
                     YCYQQALQTKNKLFILRTQHMVGVLDQEAGNYNGSILFLDSAITLAQELKDEKRLGHL
                     YNSIGMSYYLAGNYSKGMEYVIMSAKLKEKIGDVPGTITTYLNISALYEEQNDTANAR
                     KYAQQAYDVCGRSGTADLLPEVNDAMGKALLSCGDTVRGKAYLLKALDEAKKTNTPLK
                     SVTTLENLSTLALSRGDTAEARKYILAFEDACGRTQDKMHLTGLYAAYAKYFWLKKNY
                     HMSEKYSRIAIGLAGKEKPEVQAAMYDVYAKALVAQNKTVEAISALERLSALKDSASE
                     ISMKMAARRISEEYESQKNANEIRHLNETQKQNEAELERKNELIAVVVPGLVLISVFA
                     FLLFRVYRRDKRNINLLQLKNKEIEIKNNIISSRNKDISDSISYARRIQDALLGDLKT
                     KDVLFGEASSFVLYKPKDILSGDFYWFGEKDGRKIVAAADCTGHGIPGALLSIACSSF
                     LDDIISHKGVTDPAEILSELRYTVIKALKQTGAQGETKDGMDIALISIDEKNRMVNFA
                     GANNSLCIVRKNNGEKTIEKIDGDRRTVGYHLGRGLPFTGHSFSYGTGDFLYLFTDGY
                     CDQFGGTENKKFRKKSLMDILLSIADLPMDRQEEALLKAHLEWKGEEEQTDDMLVIGV
                     RMN"
     gene            3839..4447
                     /locus_tag="VFU15_08890"
     CDS             3839..4447
                     /locus_tag="VFU15_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEU4717939.1"
                     /translation="MTYIKTNIPQAGIVELLFYKGPTGNALSHLAHTILKGPSPLTSG
                     ERELIAAYVSNLNNCEFCHKSHAASANSHLGDEGKAVACVVGDIDTAPVSDKMKKLLR
                     IAGKVQISGREVTPALIEDAKKAGATDEEIHDTVLVAAAFCMYNRYVDGLNTNLPAKD
                     EDYADMGKRLSAKGYKYPPRFLLWLVRRILRKKPAELKERVR"
     gene            4524..>5298
                     /locus_tag="VFU15_08895"
     CDS             4524..>5298
                     /locus_tag="VFU15_08895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717940.1"
                     /translation="MRKFLPAGFLLLSLSCAANSGGPDAFGYTWKDNNEPGGPAYQWY
                     EISTTGTQVNGLGDDNFVGPIQIGFQFRYYWYQESKVWIGSNGYVEFGPGNLAANFPS
                     IPDPTGVNNYIGGIVSDLTFLDPTNPGKVFYKSLPDSFVVEYQNVPYWDANPPYYNQA
                     SSNTFEIILNRVDSSITVNFQSYSGTSFSNYTSGIENNIGNMGLQPLAYQPPQQAYTL
                     KYYYPINPPAVTDAGVDWNYMDGDGGIFLASQGAPYTLVT"
BASE COUNT         1243 a         1476 c         1337 g         1242 t
ORIGIN      
        1 cggttccgcc ggccctgtat ggcatccggc ccgaaaaaga gacgagaacc tgcaccatca
       61 tgggcgtccc ggtttctgtg aatgaaacga tccgtccggg ccgcattctt ttcaacgcga
      121 tcacgctgca tcccggcgat acatccgaaa cgaggtggtt cgttcaccgc gagctcgcgg
      181 aagcattctg ccaggatcat gacctcgaca cgcttttgtc gtggaaacac tgccgcctgg
      241 gccggggaac ggaccgtttt gacggaagaa cattttattc cgtttccgtc cagtcgccgc
      301 aggaagattt tccacgcatt accgattatt tcggctgtgc ccggaatatc ttttaccata
      361 tccacgtgga ggcgccgtca aaaaaaacat gggatgcctt tgccgcactg cttcacaccc
      421 tccgtttcgt ggaatttgaa cggaactgaa cacgatcgtt tccgtataac ccaacaaggg
      481 cgaaaaaagg gaaactttcg actttttaac agtttcctga caacgtgtaa ccttttaaca
      541 gcgtatacgt taaggcttaa gactcacaca caacaaaccg ttaatgaaac actaaatacc
      601 cgtcaaaccc atatgatgcg tcaattaaaa atttcgaagc aggtcaccaa ccgggaaacc
      661 gcgtctcttg acaaatacct gcaggaaata gggcgtgtgg atcttatcac cgccgaagaa
      721 gaagtagatc ttgcgcgaaa gatcaaacag ggcgaccagt acgcgctggc caaaatggtc
      781 aacgccaacc tgcgtttcgt ggtttctgtt tccaaacagt accagaacca gggactgagc
      841 ctgccggatc ttatcaacga aggcaacctc ggcctgatca aggctgcgca gcgtttcgat
      901 gaaacacgcg gattcaaatt catttcctat gccgtgtggt ggatccgcca gtcgatcctg
      961 caggccctgg ccgaacagtc gcgcattgtg cgccttccgc tgaacaagat cggttcgatc
     1021 aacaagatca acaagacgtt cgcgaagctg gagcaggaat tcgaacgcga gccgaatgcg
     1081 gacgagatcg ccgctgcgct ggaactttcg ccggaagata tccgcgaagc gctgcgcaat
     1141 acaggacgcc acgtttccat ggatgcgcct ctcgcctccg gtgacgagaa cacgagcaac
     1201 atgtacgacg tgatgcaaag cgacgatacg ccgagcccgg agaacacgct catcaacgaa
     1261 tcgctgcgga aagagatcga acgcgcgttg tccacgctta ccacgcgtga agcggatgtc
     1321 gtgcggcttt atttcggcct caacggcgaa cacgcgatga cactggaaga gatcggcgaa
     1381 cgtttcgacc tcacccgcga gcgcgtgcgc cagatcaagg aaaaagcgat ccgccgtctc
     1441 aagcatacgt cacgcagcaa actgctgaag acatatctcg gataaatcct gtccgctgaa
     1501 aaaaaatccc ggtcatgccg ggattttttt tgctcacttc atttcggtcg catccaaaaa
     1561 ttcaattcat ccgcacgccg atcacgagca tatcgtcggt ctgttcctct tcccctttcc
     1621 attcgagatg cgctttcagc aaagcttctt cctgcctgtc catgggaagg tccgcgatcg
     1681 aaagcaggat atccatcaat gatttttttc ggaacttctt gttttccgtt cccccgaact
     1741 ggtcgcaata accatcggtg aagaggtaca aaaaatcacc tgttccgtaa gaaaagctgt
     1801 gaccggtgaa gggaagtccg cggccgagat gatatcccac tgtgcggcgg tcgccgtcga
     1861 tcttttcgat cgtcttttcg ccgttgtttt tcctgacgat gcaaagtgaa ttgttagcgc
     1921 ctgcgaaatt caccatcctg tttttttcat cgatgctgat gagcgcaatg tccatcccgt
     1981 ctttcgtttc gccctgcgca cccgtttgct tcaacgcttt gatgaccgtg taacgcagct
     2041 cgctgagaat ttccgcagga tcggtaacgc ccttgtggct gatgatatca tcgaggaaag
     2101 agctgcaggc gatgctgagc agcgcaccgg gaattccgtg tcccgtgcaa tccgctgccg
     2161 ccacgatctt cctgccgtct ttttcaccga accagtaaaa atcgccggag agaatatctt
     2221 tcggtttgta gagcacaaac gaagaagctt ccccgaaaag aacgtctttc gttttcaggt
     2281 cgccgagcag ggcatcctgg atgcggcggg cgtagctgat gctgtcggat atgtctttgt
     2341 tacggctgga gatgatgttg ttcttgatct cgatctcctt gtttttcagc tggagcaggt
     2401 tgatgttcct tttgtcgcgg cggtaaacgc ggaacaggag aaaagcgaag accgaaatca
     2461 gcacaaggcc cggcaccacg acggcgatga gctcattctt acgttccagc tccgcctcgt
     2521 tctgtttctg tgtttcgttg aggtggcgga tctcgtttgc attcttctgg ctttcgtatt
     2581 cctcgctgat gcggcgcgcc gccattttca tgctgatctc gctggcggaa tccttcagtg
     2641 cggaaagtcg ttcgagcgca gaaatggctt ccacggtttt attctgcgcg acgagcgcct
     2701 ttgcgtaaac atcgtacatc gccgcctgca cttccggctt ttctttcccg gcaagaccga
     2761 tggcgatgcg gctgtatttt tccgacatgt ggtaattctt tttcagccag aaatattttg
     2821 catacgccgc gtaaagcccg gtaaggtgca tcttgtcctg cgtgcggccg caggcatcct
     2881 cgaacgcgag aatgtatttg cgcgcttcag ccgtatcccc gcgggaaagc gcaagcgtgg
     2941 aaagattttc gagcgtggtg accgatttca gcggcgtgtt cgtttttttc gcttcgtcga
     3001 gcgctttgag gagataagct tttccgcgca ccgtgtcgcc gcatgacagc aatgcttttc
     3061 ccatggcgtc gttcacttcc gggagaagat cagctgttcc cgatctcccg catacatcgt
     3121 acgcctgctg cgcatatttt ctcgcgtttg ccgtatcgtt ctgctcttcg tacaacgcgg
     3181 aaatattcag gtaagtggtg atcgttcccg gcacatcccc gatcttttct ttcagcttcg
     3241 ccgacatgat cacatattcc attcccttcg aataattccc cgcgaggtaa tagctcatcc
     3301 cgatggaatt gtagagatgt cccagccgtt tttcatcttt cagttcctgc gccagcgtga
     3361 tcgccgaatc aagaaaaagg atcgacccgt tgtaattacc cgcttcctgg tcgagcacac
     3421 ccaccatgtg ctgcgtgcgg agaatgaaca gtttgttttt ggtctggagc gcctgctggt
     3481 aacaataccc ggaaagcatc atcgccgaat ccatgttgcg gtaatgcatc ggccatatct
     3541 tcgcaaacac ttcattgatc ttgttcgtat ccggttcctg cgaaacgcca cggaaaggga
     3601 cgaacaccag caagagcaag caggcaaaaa acattcgcgc tccgcgcagc agggcatccg
     3661 gacttttttt cacgatcata aacaactgcg ttcggggtga gccatgcaaa tatcgaaaaa
     3721 tatacaggga aatggtcaga gatgtaaata cgggaacgcg ggagaagaga cgccagaccg
     3781 tgaaagcggc gggaagattt ttcttttgga tacatttgtt tacaaacaac ccgttcccat
     3841 gacgtacatc aagaccaata tcccgcaggc cggaattgta gagctgctgt tttacaaagg
     3901 ccctaccggc aacgcattgt cgcatcttgc acatacgatc ctgaaaggac cttctcccct
     3961 cacctccggt gagcgcgagc tgatcgctgc ctacgtttcg aacctgaaca actgtgagtt
     4021 ctgccacaag tcgcacgcgg catcggccaa ttcacacctc ggcgacgaag gaaaagccgt
     4081 ggcctgcgtg gtcggtgata tcgacacggc ccccgtttcc gacaaaatga aaaagctcct
     4141 gcgcattgcc gggaaagtgc agatcagcgg aagggaagtc acacccgccc tgatcgaaga
     4201 cgcgaaaaaa gcaggggcga ccgacgaaga gatccatgac accgtgctcg tcgcggccgc
     4261 gttctgcatg tacaaccgtt atgtcgacgg gctgaacacc aatctgcctg cgaaagacga
     4321 agactatgcg gacatgggca agcgcctttc tgccaaaggc tataaatatc ccccgcgctt
     4381 cctgctctgg ctcgtgcgac gcatcctgcg caaaaagccg gccgagctga aagaacgcgt
     4441 tcgctgaacg cctcagtacg catgaagatg cgatctttcc gccccttttt tttgtaatat
     4501 agtagtcggt taagtcgacc ttcatgagaa aatttttacc ggcgggattt ctcctgcttt
     4561 cattgtcatg tgctgcgaat tcaggcggac ccgacgcatt cggttatacg tggaaagaca
     4621 acaacgaacc gggcggtccc gcttaccagt ggtatgaaat ttcaacgaca ggaacgcagg
     4681 tgaacgggct cggcgacgac aacttcgtcg gtcccatcca gatcggtttc cagttcaggt
     4741 attactggta ccaggaaagc aaagtgtgga tcggttcgaa cggctatgtc gaattcggcc
     4801 cgggaaatct tgcggcgaat ttcccttcca ttcccgatcc gacaggcgtg aacaattaca
     4861 tcggcggcat tgtttccgac ctcacgtttc tcgacccgac gaatcccggg aaagtttttt
     4921 acaaatcgct gcccgacagc tttgtcgtgg aataccagaa cgttccgtac tgggacgcga
     4981 acccgcctta ttacaaccag gccagctcga acacgtttga gatcattctc aaccgcgtcg
     5041 actcttccat caccgtcaat tttcaatctt actccggaac ttcattcagc aattacacga
     5101 gcgggatcga gaacaacatc ggcaacatgg gactccagcc gctggcctac cagcctccgc
     5161 agcaggcgta cacgctgaaa tattattacc cgatcaaccc gccggcggtg accgatgcag
     5221 gcgtcgactg gaattacatg gacggggacg gcggaatttt tctcgccagc cagggcgcgc
     5281 cgtacacgct cgtcacgc
//
LOCUS       DASTSF010000311         2493 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_16958,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000311 DASTSF010000000
VERSION     DASTSF010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2493
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16958"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            296..868
                     /locus_tag="VFU15_08900"
     CDS             296..868
                     /locus_tag="VFU15_08900"
                     /inference="COORDINATES: protein motif:HMM:NF013814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HEU4717941.1"
                     /translation="MKKILLFGSAFLALVFAFSSCSMEKRHYMKGYHIEWFDQAKKTD
                     ANTTQAVVARQETVTPVSIQPKQASSAVAAANTVPVPVATVKPARSFTPAGSGNAKTV
                     TARNTAAKVQHAAASHANTLVNKAAKSSKNGGPDKALLIVLCFFIPWLAVGLATDWDV
                     KRVIICLLLGFLLCIPGIVYAIIQVNKEVG"
     gene            927..1187
                     /locus_tag="VFU15_08905"
     CDS             927..1187
                     /locus_tag="VFU15_08905"
                     /inference="COORDINATES: protein motif:HMM:NF022281.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2752 domain-containing protein"
                     /protein_id="HEU4717942.1"
                     /translation="MLIILPADYFDHGESICPSVIFFNRECFGCGTTRATMHMLHLQF
                     REAWHFNRLSFALVPFLLYMYVSLLIKFTRRARYPDGKPKQL"
     gene            complement(1306..2331)
                     /locus_tag="VFU15_08910"
     CDS             complement(1306..2331)
                     /locus_tag="VFU15_08910"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010602140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HEU4717943.1"
                     /translation="MNIDSLLEKNLLPDSVIRPGIRRLLKERLRDEKKSSPGEQQEHF
                     NSLLEVCRNSPLAIETQAANEQHYEVPTEFYLHCLGPNLKYSSAFYRPGVTSLEQAEK
                     DMLALTCQRAELENGMEILELGCGWGSLTLWMGKHFPGAKITAVSNSRTQKEFIDGEA
                     KKRGISNITIITADMNVFSTEKKFDRVVSVEMFEHMRNHGLLLKRISGWMKDDAKLFV
                     HIFVHRDYLYFFEVKNERDWMSKYFFTGGMMPSDHYLYYWNDDLRVKKHWRVSGIHYS
                     KTSEAWLANMDAEKKKIIPLFEKTYGKKEARKWWVYWRVFFMACAELWGYADGGEWFV
                     GHYLLEK"
BASE COUNT          560 a          672 c          596 g          665 t
ORIGIN      
        1 gcatggactg tatttcccat acgccggtat tgcgccagag gaatttctcg tcctttttgc
       61 aggaatgaaa aacgagcgca agaaggaaaa gtaagggtaa gtattttttc gtcatacgga
      121 tcgcgcaaat gtgggaagaa aacaggtaca cggacatcgc aaaaaagtag taggcgccct
      181 tttttttaat ttgattctaa tagttgtgca aacgctttgt taaaagaccg ctcttttccc
      241 tgaaactgca tatattgtcc tgaccaaaaa aattaaccca ctaatctaac tttctatgaa
      301 aaaaattctc ctattcggct ctgcgttcct cgcgcttgtt tttgccttca gctcctgctc
      361 gatggaaaag cgccattaca tgaaaggcta ccatatcgaa tggtttgacc aggcaaagaa
      421 aacggatgcg aacaccacgc aggctgtggt cgccaggcag gaaaccgtca ctcccgtttc
      481 cattcagccg aaacaggctt cctctgccgt tgctgctgca aacacggtac cggtgccggt
      541 tgcaactgta aaacctgcgc ggtcgttcac accggccggc agcggaaatg cgaaaacagt
      601 tacagcccgt aacactgccg cgaaagtgca gcatgctgcg gcttcgcacg cgaacacgct
      661 ggtgaataag gcggcaaaaa gctccaagaa cggcggaccc gacaaagctc ttctcatcgt
      721 tctctgcttt ttcattccct ggcttgcggt aggtcttgcc accgactggg acgtgaagcg
      781 cgtgatcatc tgcctgcttc tcggcttcct cctctgcatc ccgggaatcg tttacgcaat
      841 tatccaggtc aacaaggagg tcgggtaaaa caatgcgccg ggttttcttc atcgtattag
      901 tggcgggaat gattataaca ccgattgtgc ttataattct tcccgccgac tattttgacc
      961 acggagaatc catttgtcct tccgtcattt ttttcaacag ggaatgtttc ggctgcggga
     1021 ctacacgggc aacgatgcac atgctccacc tgcaattcag ggaagcgtgg catttcaaca
     1081 ggctcagctt cgcactcgtt cctttcctgc tttacatgta tgtgagcctg ctgatcaaat
     1141 tcacacggcg ggccaggtat ccggacggaa aaccgaagca gctttgaacc tgttttgagc
     1201 gtatccgaaa ctaatcgtgt tcatcccaat tcccaatctc ccgaaacacg gaccgtttct
     1261 tcccccggga tttcagcgat gtttttttga gatgattttc ctgcgctatt tttccagcag
     1321 gtaatgcccc acgaaccatt ccccgccgtc cgcataaccc catagttccg cgcaggccat
     1381 aaagaacaca cgccagtaca cccaccattt ccgcgcttcc tttttcccgt aggttttttc
     1441 gaaaagcggg ataattttct ttttctctgc gtccatgttc gcgagccagg cttccgatgt
     1501 tttcgaataa tggataccgg aaacgcgcca gtgctttttc acgcgcaggt catcgttcca
     1561 gtagtagaga taatgatcgg aaggcatcat tcctcccgtg aaaaaatatt tcgacatcca
     1621 gtctctctcg ttcttcactt cgaaaaaata gagataatcg cggtggacga aaatgtgcac
     1681 gaacagcttc gcatcgtcct tcatccatcc cgagatccgt ttcagcagca gtccgtgatt
     1741 gcgcatgtgc tcgaacattt ccaccgagac gacacggtcg aatttttttt cggtcgagaa
     1801 gacgttcatg tcggccgtga tgatggtgat gttggaaatg cctcttttct tcgcttcacc
     1861 gtcgatgaat tctttctgcg ttcgcgagtt ggaaacagcc gtgatcttcg cgcccggaaa
     1921 atgttttccc atccacagcg tgagcgaacc ccagccgcag ccgagctcga gaatttccat
     1981 tccgttttcc agttccgcgc gttggcaggt gagcgcgagc atgtccttct ccgcctgttc
     2041 cagcgacgtg acgccaggtc tgtaaaacgc gctgctgtat ttgaggttcg ggcccaggca
     2101 atgcaggtaa aattccgtcg gcacttcata gtgctgctcg ttcgcggctt gcgtttctat
     2161 ggcgagcggg gaattccggc agacctccag caatgaattg aaatgttcct gctgctcgcc
     2221 gggagaagat tttttttcat cgcgcaggcg ttccttcagc aggcggcgaa tccccgggcg
     2281 tatgacactg tcgggaagga ggtttttttc aaggagagaa tctatgttca tcaatgacgg
     2341 gcttccctgt ttcggttttt cggcgttgat ccgttatgcc tggtgtttct tcctccacgg
     2401 cacaaacacg cttgtcgtgc gctggtattc tttgtacgca tcgcccttcg aacgcacggc
     2461 ctgctcttcg gtcatgggaa tgcccgtgac ctt
//
LOCUS       DASTSF010000312        16976 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_490289,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000312 DASTSF010000000
VERSION     DASTSF010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 16976)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16976)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16976
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_490289"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..542)
                     /locus_tag="VFU15_08915"
     CDS             complement(<1..542)
                     /locus_tag="VFU15_08915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="HEU4717944.1"
                     /translation="MKNPFGNSFSDQWLRRVFFACAAVMLLIMLSVSGRYGVCWDEKT
                     QKDYGDSVLNFYTSFGKDTGYINRQNHIHTYGGFVEVVCSVATKISSADPYDTRHFII
                     AWFGFFAMLFAGLVAKEIAGWKAGIVSLLFVFLTPVFFGHSMFNSKDIPFAACYTAGI
                     YFLIRFLKELPSPSRKSVIYL"
     gene            complement(539..1264)
                     /locus_tag="VFU15_08920"
     CDS             complement(539..1264)
                     /locus_tag="VFU15_08920"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU4717945.1"
                     /translation="MPETGQSWSVLVFCYNEAGTVKQVAESVLDFFHKIHCDDHEIII
                     VDDGSTDGSAAIIDTFAKEHPSVRVIRHEKNRGIGQALLSGYFAATKENITAVPADAQ
                     FDVNELSAFPEVEKGTFVSFFRKENTVYSLTRNILSWFNKMVNRVFLGIKLRDVNWVK
                     IFKREELVQLDLKMKSSLVESEICSKLLIRNNKVIETHSVYHRRRSGVSKGAGGKVLR
                     QAMAETLKLIFIISLYRFKKFPA"
     gene            complement(1264..2385)
                     /locus_tag="VFU15_08925"
     CDS             complement(1264..2385)
                     /locus_tag="VFU15_08925"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006988939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEU4717946.1"
                     /translation="MKRKSEMEKRIDFLDLKKQYADIREEVLRAIENVCENTAFSSGV
                     FVTQFENNFASFLGGNVNVAGVNNGTSALHLCMLALGIGPGDEVIVPANTFIASAWGP
                     CHAGATPVFTDCDPLTWNIDVKQVRERITKKTRAIIGVHLYGQPCELDELRSIARENN
                     LFLVEDCAQSHGAKYKGKSTGTTGDISAFSFYPGKNLGAYGEAGAVVSHEKTYIDKVR
                     MLINQGSGKKYYHETIGYNMRMDGIQAAVLDVKLRHLAAWTKRRQAIAKIYRENIRNP
                     KIRMQETPPYAEHVYHLFVITSDDRDGLMSYLNAHHIFPGIHYPVPIHLQKAFAHLGY
                     RKGDFPNAEFLAEHCLSLPLYPELSDDSVQYVIEKLNSW"
     gene            complement(2369..2860)
                     /locus_tag="VFU15_08930"
     CDS             complement(2369..2860)
                     /locus_tag="VFU15_08930"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002178532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEU4717947.1"
                     /translation="MKILNTDSDRQALKDVRVGENVKIFNFVNAYGCEIGDNTKVGSF
                     VEIQKGASIGKNCKISSHTFICEGVKIGDDVFVGHNVSFINDKYPQAVNESGELQTEA
                     DWKVVETFVGNGASIGTSATILCGVRIGEKAIVGAGAVVTRDVPANAVVYGNPARIHE
                     AKK"
     gene            complement(2857..3861)
                     /locus_tag="VFU15_08935"
     CDS             complement(2857..3861)
                     /locus_tag="VFU15_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004428848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU4717948.1"
                     /translation="MIRIGVIGYGYWGPNLVRNFSQLNDCVVKTVADTRPGRLEALHK
                     IYPSIAVTADYKTVLSDAEIDAVVIAVPVFLHFPVAKEALEKGKHVLIEKPMTTSVAQ
                     AEELIALAEKKRRTLMVDHTFVYTGVVQKIKSVIASGEIGDLNYFDSTRINLGLFQHD
                     INVLWDLAPHDISILNFLTGEKPVSVSATGISHTGNGIENIAYLTLHYSNNFIAHFHC
                     SWTSPVKIRKILIGGSRKMIVYDDVEPTEKIKIYDTGYSMRTDEDKHKLLVDYRTGDI
                     YIPKTEQREALLGVAGDFVSAIRENRQTLAGGTAGLEVVRVLEAAGRSLREKGKEITL
                     "
     gene            4038..4172
                     /locus_tag="VFU15_08940"
     CDS             4038..4172
                     /locus_tag="VFU15_08940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717949.1"
                     /translation="MAKISVVEQAESVQEKTGGAAVQFLTPFSVFVLSGRPDSNFIED
                     "
     gene            4304..5233
                     /locus_tag="VFU15_08945"
     CDS             4304..5233
                     /locus_tag="VFU15_08945"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEU4717950.1"
                     /translation="MKHLHFLRIAAVALPLALPALVNGQDFLNGSFEKNGNLCLINTS
                     PTVFNANVKNTKSFGSFRQPDIASSNCGFGSAKDGNWFVGLATNVQGDVRSEAITMEL
                     SSPVAKGGQYTLDFWARLRSFAPNLQLGVSNADSSCGTVFYTVSSKSIGNDWTEISVR
                     FTAPVSGKYISVRAVNANENSGVWVDAFRLSNVFIADNVIMTVKPADASKAPDNVKTA
                     SVAAAKKTDDNQVGLYPNPSDGIIKVSADSAQLVSLTVYNMLGSQVEQHIATPDQPIP
                     DKIDLTDQQPGLYFVEMATIDGSKFTKRIIISR"
     gene            complement(5311..6723)
                     /locus_tag="VFU15_08950"
     CDS             complement(5311..6723)
                     /locus_tag="VFU15_08950"
                     /inference="COORDINATES: protein motif:HMM:NF019284.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HEU4717951.1"
                     /translation="MKYAPAFFTAAAFLLFSPVGYCQSGGDVKCSDSPLYSTSGTLTV
                     TNDKFPEMDADDAHHIHAGDRGEMSKYFETEIFNSSMTGWISLGEVEVISVKDDHVKF
                     RVISKTLQVTQDGKDKETFVKGKTVKFVSYDYGVPRIDSAVYAGSGGKLKYKGTLVCG
                     KETGTWNYYYENGQVSRSYTKDSDGKMEDLFEEFYESGQKQTSGTYKTGKKEGVWTTW
                     YENGKKQSEGNFTDGVQTGKYTTWYKDGTIKEQQEYDANGNKTSMWTSYYSDGKKKEE
                     TDYSGDGKNYVTKTYYQNGQLKISASYKGNDLNGPYKSYFDDGQLKEDFIYQDGQKNG
                     ACTTYYRNGRKESQATYSGGDLSGDYQTWYDNGNVKEKGTYVNGKQNGPWEAYFDNGK
                     TKEKSTYAVGRYQGDFIEYYENGQLKSKGAYDHGNETGAWVGYYDDGKKKFEGKYKDG
                     SKTGKWTTWDSYGKKQVQKY"
     gene            6903..8414
                     /locus_tag="VFU15_08955"
     CDS             6903..8414
                     /locus_tag="VFU15_08955"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEU4717952.1"
                     /translation="MKTGLLFLFLVIAVGVGAQNYHAYVPGRTAFFSQHLYNQDYSVD
                     AISPDTSFVSGNYTVYHDYRREVTSSFSFSCLTVNDTGWAGKYFLQDANGVTCFLNKA
                     NDSIWFFPSAAVGQDGFIFPFANGDTLHGTVVSLAIQNVLGAPDSVKTMQLQRKDGSG
                     NSVADDLNGKFIEVAKDHGCILAYSWRDFPDDTTARTLAGMSSPQEGVRVVPAADVFN
                     FSIGDQFDIVDANNSNAMQLPSMMSYYTWVVTAKTVSANGDTIRYTMDENYCRKIVNS
                     IQAQHMNTPVIYTFILSEYERNFELSLMPGQYANGPDTANSLFYMDGASFFPLNATQY
                     NGRMQKGYSSRQAGYYYDQNSQCFLWDGDSPDICDGINEIYADGIGKVYAHDGSATCF
                     DNYQLVYFSKGAETWGTPNNWPVILGNNELSAPVVRCWPDPAQDVLNVDAGADFNGSI
                     HYQILDYSGRVVSEANLSASGSVIQIPVNELAHGFYIFKAETGGQTIYSRFSH"
     gene            8466..9278
                     /locus_tag="VFU15_08960"
     CDS             8466..9278
                     /locus_tag="VFU15_08960"
                     /inference="COORDINATES: protein motif:HMM:NF022750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HEU4717953.1"
                     /translation="MKRILSYFLLLGLVPQGGYAQSTGTGSTSTGTNDYRKVTNKAFK
                     RGEELHYRVHYGAINAGVADMTVESDNKLINNRPTFHMVGVGSSKGAFDWVFKVRDRY
                     ETYMDEEALMPWLFIRRVDEGGFKINQNQSFDHNGGKVVSNGKSLTVPKYIQDMLSSF
                     YYARTMDFSKAKVNDIFSVPTFVDDEIWDLKIKFLGREAIDTDVGKVNCLKFCPVVQK
                     GRVFKKEEDLTVYISDDDNHIPVRAQGEILVGSVKLDLMSYSGLAANLNMAK"
     gene            9384..10316
                     /locus_tag="VFU15_08965"
     CDS             9384..10316
                     /locus_tag="VFU15_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014781429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase family protein"
                     /protein_id="HEU4717954.1"
                     /translation="MSEAGNKHKDILEKIENKYNNLGQDAGLFLKGFSYQRPVTYWDY
                     VQLETLLSLQRPRTDFPDEPIFIMYHQVTELLLKLIMHELNQAVHNESVTPQFFTTKM
                     QRVNRYADILTQSFSVMREGMDYEQYNEFRLALAPASGFQSAQFRQIEITCTDVLQLL
                     NHRVKGTITDDSDLDKIFSELYWKDAGLDRKTGKKTQTLQLFEEKYENDLKHLATRMK
                     GKNIYRRFLAMEKEGANVHELKNALRNFDHTYNVKWPLSHLKTAEYYLNSKGEGKAAT
                     GGSEWQKYLHPSYQRRIFFPALWSEDELAKWGNF"
     gene            10407..11918
                     /locus_tag="VFU15_08970"
     CDS             10407..11918
                     /locus_tag="VFU15_08970"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEU4717955.1"
                     /translation="MKRSLLFLFLLPAVVTAQNWSPLNLTDKYNYRLGNDPVITQTVW
                     QNGFSAVAGDTVFTLNDIMCDTCLTWTSGPLACDSCYGLLHVAQFEGKQVKQFASGWC
                     NFRDPGSKTIDLFAALNDTWLFDTLNNVTAQVIFAGNSVVLGNADSVKTLLLSTGDTL
                     CFSKDYGIVQWPHGYGQNSYYRLAGIQGRNIGVLIPRMMNYFDFAVGDIFQYQGRINS
                     GASPQVRDFIRKYIITGKTVNGDTVRYTVQGHMASQCEDVISMNFWMEYSLINETYTF
                     IDSANHTGNQFNNCAVDGFLRVIPNSGGMGQPQNSFNPAPGWYRCSLYMDPFGNKGIR
                     FGTATSYTDFHIDPSCSVPVFAGSDTLQPVSWGDAFTVQLTEGLGQTFALWTSNFEGS
                     WSEQLTAFRKNGDTTGVLIPDNQLDALAQHPGSGGGMTVYPNPADEALIISLPGNVQA
                     DLVITDAGGRVVFRQQNAAGNIQLATADLAEGMYFLRVITAQGAAAEKIVIRH"
     gene            12107..14554
                     /locus_tag="VFU15_08975"
     CDS             12107..14554
                     /locus_tag="VFU15_08975"
                     /inference="COORDINATES: protein motif:HMM:NF039501.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5686 and carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEU4717956.1"
                     /translation="MLCPALHAVTLSGKVTSPAGEALGYAAVYVKGTSKGTTTNTEGD
                     YTLDLTPGNYTIIFQMMGYYQKQFDVDLTQGNKTLDVVLTPTTLQLKEAVITDGEDPA
                     YAVIRNAIRKRKYYRDQVDAYTCDVYIKGLQRVTQHPQKIMGMDVDPDGDIDSASGIV
                     YLSESVSTFNFRQPDKIKEEMVSSKVSGDNKAFSYNRASDMLFNFYDNVIEIEDLSER
                     GFVSPIANNALFFYKFRMAGTIMDNDSMVNKIEVIPKRKTDPCFHGYIYIMENSWRIY
                     ATDLYLTKDAQIDFVDTLTISQTYIPVEKDVWMVFMNKFTFVFGFMGLQGNGYYVGVN
                     KDYVINPDFPKKFFDNEEMHVADDANLKDSAYWQTTRPVPLTDEEERDYKRRDSLMQI
                     RNSKPYLDSIDKRTNRPGFTDIFLTGYSYSRRYNHFSVSVSPVVRAFSYNTVQGWRTG
                     LDVDAFKSWDNNRWLSVGPSASYGIGDKKFYVDGEATYYYKPVRFARVTAAGGTTTAQ
                     FSPRDPVPVFINTIYTLGEKQNYMKLYAKKYGSLNWFGEVTNGVMMNINLEYADRTPL
                     YNTSFYYWNWRFGPSSLPAPGYLETRRFTSNNPLDPSDNSSAAFGAHRSLDLNAQAII
                     RFKQKYYTRPNEKIILGSKYPAIGIQYRRGIPGIFGSKTDYDFLKLSVRDEINLKLMG
                     KFRYYFAVGKFLSQKYLPFMDYYHFQGNRTFWSTFAQQSFQLLDYYTYSTNTWFMEAH
                     AEHHFNGFLFDKIPLLKKMHLTEIVGVNWFMTDRVPSYYEIYFGADKLGLIRVDFALG
                     YMQNAKVSAGLRLGIRI"
     gene            14567..15910
                     /locus_tag="VFU15_08980"
     CDS             14567..15910
                     /locus_tag="VFU15_08980"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEU4717957.1"
                     /translation="MNTMNNRVIPGSRKLVLFPLLLLISIGAHAFVYQTFDKTLSNGL
                     KVITCEKPGSGYAEVEVWYRVGSKDEHAGIRGMAHMFEHMMFRGTKNVSGEQLLEKLD
                     SSGAQWNAYTTFDRTVYHEYLPVNAVSMALYLEADRMSNLVVTQEILNTEREVVGEEY
                     RNGMSNWYEKMVEDRYEYLYPDGHPYQYDVIGHLDEITNFTAPECMDFYDKYYSPNNA
                     FVVVVGDVKHDDIFAQCEKYFGPITKQLPAVVPGPSPDIMGTHVKTTDMELNFPLQIY
                     CYSLSQPAAGSKDFFAFNMLADIFFTGDNSILNERLVKKEHSVYFINQSNEAWSLYPS
                     RTQFDVFMPPSPGNVKVKKAIRAEMDSVIANGLPQEKVNDFISNMEAQQTLGAYSSEN
                     IAGELGMAEYYFHDYNRAKTIVNDYKAVTQDDIKRVAATYFSPDHVDVINIKPSF"
     gene            15912..>16976
                     /locus_tag="VFU15_08985"
     CDS             15912..>16976
                     /locus_tag="VFU15_08985"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEU4717958.1"
                     /translation="MKKRSLFRLTLVPVALLLLASAAQDKGPFPDIERYKLKNGLEVI
                     FADYGDLPVTSLTFFVNVGKKSETPGQQGLAGLTANSLLLGNDKFSRTDQDRMLYRLG
                     TGISADANENFTTVSGEFLNTDIGKGFEIVSAVLLHPTFPQQDIDEEKNLDISQNKPS
                     KMDIGDLASMYGNYFCYGIGHPLGRHFYEAQYKKITTAQIRDFYNFNYTPGNTKLVVC
                     GKPDREKVKALIEQYFGGWNATYGEVNGSSYDIPPIKTREFAFVPKDSAKQACLLWMK
                     KGPAAGSKDMTAFIIANAVFSDHLTKEIREKRGYTYGIYSTFSQAQDDGIFRARTQVR
                     NDVMFATMTAFDNVLTEFYAK"
BASE COUNT         3975 a         4520 c         4474 g         4007 t
ORIGIN      
        1 aggtaaatga ccgatttccg ggaaggcgac ggcagttctt tcaggaagcg gatcaggaaa
       61 taaatgcccg ccgtgtagca cgcagcaaag ggaatgtcct tcgagttgaa catcgaatgt
      121 ccgaagaaga cgggcgtgag aaagacgaag agcagcgaca ctattcccgc tttccaccct
      181 gcgatctctt tcgccacgag cccggcgaac agcatggcga aaaaaccgaa ccacgcgatg
      241 atgaagtggc gcgtgtcgta cggatcggct gaagaaattt ttgtggcgac ggaacacacc
      301 acttccacga aaccgccgta ggtgtgaatg tggttctggc ggttgatgta ccccgtgtcc
      361 tttccgaagc tcgtgtagaa attcaggacg ctgtcgccgt aatccttctg cgttttttcg
      421 tcccagcaaa cgccgtaacg gccgctcacg gaaagcatga tgagcagcat caccgcggcg
      481 cacgcgaaaa atacccggcg aagccactga tccgaaaaag aatttccgaa tggatttttc
      541 acgcggggaa ttttttaaag cggtacaaag aaatgataaa aatgagcttc agcgtttccg
      601 ccatcgcctg gcgcagcact ttgcccccgg cgcctttgct cacgcccgat ctgcgccggt
      661 gataaacgga atgggtctcg atcactttgt tgttccggat caggagcttc gagcagatct
      721 cgctttccac gagcgagctt ttcatcttca ggtcgagctg cacgagttct tcccgcttga
      781 atattttcac ccagttcaca tcgcggagct ttatgccgag aaaaacccgg ttcaccattt
      841 tgttgaacca ggaaaggatg ttccgcgtga gggaatacac cgtattctcc ttgcggaaaa
      901 acgaaacgaa agttcctttt tccacttccg ggaaagcgga cagctcgttc acgtcgaatt
      961 gcgcgtctgc cggaacagca gtgatgttct cttttgtcgc ggcgaaataa cccgacagca
     1021 gggcctgccc gatgccgcgg ttcttctcgt gacggatgac gcgcacggaa ggatgctctt
     1081 ttgcaaacgt gtcgatgatc gcagccgatc cgtccgtgct tccgtcgtcg acgatgatga
     1141 tctcgtgatc atcgcagtgg atcttatgga aaaaatccag cacggattcc gctacctgct
     1201 tcaccgttcc ggcttcgttg tagcagaaga caagcacgct ccaggattga ccggtttccg
     1261 gcatcaccag ctgttgagtt tttcgatcac gtactgaacg gaatcgtcac tgagttcggg
     1321 ataaagagga agggaaagac agtgctccgc gagaaattcc gcattgggaa aatcgccttt
     1381 gcggtaaccg agatgcgcga acgctttttg taaatgaatg ggaacagggt aatgaattcc
     1441 cgggaaaatg tgatgggcgt tcaggtagct catcagcccg tcgcgatcgt ccgacgtgat
     1501 cacgaaaaga tgataaacgt gctctgcgta cggcggtgtt tcctgcatgc ggatcttcgg
     1561 gttgcggatg ttttccctgt atatttttgc gatcgcctgc cggcgtttcg tccacgcggc
     1621 aagatggcgc agcttcacgt cgagcaccgc cgcctgtatg ccgtccatgc gcatgttgta
     1681 accgatggtt tcgtggtaat atttttttcc ggaaccctgg ttgatgagca tgcgcacctt
     1741 gtcgatgtat gttttttcgt gcgagaccac agccccggct tccccgtagg cgccgagatt
     1801 tttcccggga taaaagctga atgcggaaat atcgccggtc gtgcccgtgc tttttccctt
     1861 gtatttcgcg ccgtgcgatt gagcgcagtc ttccacgaga aaaagattgt tctcccgcgc
     1921 gatgctgcgc aattcatcga gttcgcaggg ctggccgtac aaatggacac cgatgatggc
     1981 gcgcgttttt ttcgtgatgc gctcacgcac ctgcttcacg tcgatgttcc aggtgagcgg
     2041 atcgcagtcg gtgaaaacgg gcgttgcgcc tgcatggcac ggaccccatg cgctggcgat
     2101 gaatgtgttc gccggaacga taacttcatc gccaggaccg atgccgagtg cgagcatgca
     2161 gagatgcagc gccgacgtac cgttgttcac gccggccacg tttacgtttc cgccgaggaa
     2221 tgaagcgaaa ttattttcaa actgcgtcac aaaaacgccg gaagagaaag ccgtgttctc
     2281 gcagacgttc tcaatggcgc gcagcacttc ttcgcggata tccgcatact gttttttcag
     2341 atcgagaaaa tcgatgcgct tttccatttc actttttcgc ttcatgaatg cgtgcgggat
     2401 ttccgtaaac gacggcattg gccggaacat cacgcgtgac gacggcgccc gcgcccacga
     2461 tcgctttttc gccgatgcgc acgccgcaga ggatcgttgc gctcgtgccg atcgatgcgc
     2521 cgttccccac gaaagtttca acgactttcc agtcggcttc cgtctgcagc tcgccggatt
     2581 cattcacggc ctgcgggtat ttgtcgttga taaaagaaac gttgtggccg acaaaaacat
     2641 cgtcgccgat cttcacgcct tcgcagatga acgtgtggct cgagatcttg cagttcttcc
     2701 cgatcgaagc gcctttctgt atctcgacga acgagcccac tttcgtgttg tcgccgatct
     2761 cgcagccgta agcgttcacg aagttgaaga tcttcacgtt ctccccgacg cgcacgtctt
     2821 tcagcgcctg ccggtccgaa tcggtattga ggattttcat agtgtgatct cttttccttt
     2881 ttcacgcagc gatcgcccgg ccgcttcgag aacacggacg acctccagtc ccgctgttcc
     2941 gccggccagc gtctgcctgt tttcccggat ggctgaaacg aaatcgcctg cgacgccgag
     3001 cagggcttcc cgctgctccg ttttcgggat gtaaatatcg ccggtgcggt aatcgacaag
     3061 gagtttgtgc ttgtcttcgt cggtgcgcat ggaatagccg gtatcgtaaa tcttgatctt
     3121 ttccgtgggc tcgacgtcat cgtaaacgat catttttctg ctcccgccga tgaggatctt
     3181 gcggatcttc acgggcgatg tccacgagca gtgaaaatgc gcaatgaaat tattgctgta
     3241 atgcaacgtc aggtaagcga tgttctcgat gccgttgccg gtgtggctta ttcccgttgc
     3301 cgaaacggaa accggttttt cgccggtgag gaagttcagg atggagatat cgtggggcgc
     3361 aaggtcccag agcacgttga tgtcgtgctg gaaaagcccg aggttgatgc gcgtggaatc
     3421 gaaatagttg aggtcgccga tctcgcctga agcgatgacg gattttattt tctgcaccac
     3481 gcccgtataa acgaacgtgt gatccaccat cagcgtcctg cgtttttttt ccgccagcgc
     3541 gatcagctct tcggcctgcg caacggaagt cgtcatcggc ttttcgatca gcacgtgctt
     3601 gcctttttcc agcgcttctt ttgccaccgg aaaatgaagg aacaccggta ccgcgatgac
     3661 taccgcgtca atttccgcgt cgctgagaac ggttttgtaa tcagcggtaa cggcaatgga
     3721 gggatagatc ttatgcaggg cttccagtct cccgggacgc gtgtccgcca ccgtttttac
     3781 gacgcagtcg ttcagctggc tgaagttccg cacgagattc gggccccagt aaccataacc
     3841 aataacgccg atccggatca tgtgagggtt tgcaggaaat taacggtagc caaaatagga
     3901 atttttgagg gaggacttac gggctgcgcc atcactttga tgacggcagg gtgacgcaat
     3961 gagaatcccc accgatcccg ccaaccattg cggatggaat gcgtggcgcg gaaataaccg
     4021 gcggaaattc atccgccgtg gcgaagatct ccgtggtgga acaagctgaa tccgttcagg
     4081 aaaaaaccgg cggtgcggct gtacaatttt taacaccttt ttccgttttt gttttatccg
     4141 ggcgaccgga cagtaatttc attgaggatt agcgacttaa tcacataatg ccggtgtggc
     4201 atgcggattg atcggttggg gaagtagccg cccggtgtaa ctttttttaa cgggatgagt
     4261 tatactaaaa caaggtattc accaacaaga agtctcccac accatgaaac acctccactt
     4321 cttgcgtatt gcagccgtag cattgccact cgcgcttccc gcgctggtga acggccagga
     4381 ttttctcaac ggaagttttg agaagaacgg caatctctgt ctcatcaaca cgtcgccgac
     4441 ggttttcaac gcgaacgtga agaacacgaa atcattcggc agcttccgcc agcctgatat
     4501 cgcgagcagc aactgcggct tcggttcggc gaaagacggc aactggtttg tcgggctcgc
     4561 taccaatgtc cagggcgacg tgcgttcgga agcgataacg atggaacttt catcgcctgt
     4621 cgcgaaaggc gggcagtata cgctcgattt ctgggcgcgc ctgcgttcgt tcgcacccaa
     4681 cctgcagctc ggcgtcagca acgcggattc ttcctgcgga accgttttct atacggtttc
     4741 ttcaaaatcc atcggtaacg actggacgga gatcagcgtg cgtttcacgg ccccggtcag
     4801 cggtaaatac atttcggtaa gggccgtcaa tgccaatgaa aattccggtg tctgggtcga
     4861 cgcgttccgc ctgtcgaatg ttttcatcgc ggataacgtg atcatgaccg tgaagcccgc
     4921 ggatgcttca aaagcgccgg ataacgtgaa gacggcttcg gttgccgcag cgaaaaaaac
     4981 agacgacaac caggttggac tttatcccaa tccttccgac ggcattatca aggtgagcgc
     5041 cgacagcgca cagctggtga gcctgacggt ttacaacatg ctcggctcgc aggttgagca
     5101 gcatattgcg acaccggacc agccgatccc cgacaagatc gatctcaccg accagcagcc
     5161 cggactttat ttcgtggaga tggcaaccat cgacggaagc aagttcacga agcggatcat
     5221 tatctcacgc tgagaaaaaa ataaaaagag aaaaaaacag gccgggaaat tttgtcccgg
     5281 cctgttcttt tttgttcggc gcatgcggca tcagtatttc tgcacctgtt tctttccgta
     5341 ggaatcccac gttgtccatt tgccggtctt gctcccgtct ttgtatttgc cttcgaattt
     5401 ctttttcccg tcgtcgtaat aacctaccca tgcgccggtt tcgttcccgt gatcgtacgc
     5461 gcctttgctt ttcagctggc cgttttcgta atattcgatg aagtcgccct ggtaacgtcc
     5521 gacagcgtag gttgattttt cttttgtttt cccgttgtcg aaatacgctt cccacggtcc
     5581 gttctgcttt ccgttgacgt acgttccttt ttccttcacg ttgccgttgt cgtaccacgt
     5641 ctggtaatcg ccgctgagat ctcctccgct gtaggttgcc tgcgattcct tcctgccgtt
     5701 cctgtaatac gtcgtgcagg cgccgttctt ctgcccgtcc tggtaaatga aatcttcctt
     5761 cagctgcccg tcatcgaaat aggatttgta cggaccgttg aggtcattgc ctttgtacga
     5821 cgcagaaatt ttcagttgcc cgttctggta ataggttttg gtgacgtaat ttttcccgtc
     5881 gccggaataa tccgtttcct cttttttctt tccgtcggaa taataggatg tccacatgct
     5941 ggtcttattt ccgttcgcat cgtattcctg ctgctctttg atcgtgccgt ccttgtacca
     6001 ggtcgtgtat ttccccgtct gcacaccatc cgtgaaattt ccttccgatt gtttttttcc
     6061 gttctcgtac cacgtcgtcc acacgccttc ctttttcccc gtcttgtaag taccggaagt
     6121 ctgtttctgc ccgctctcgt aaaattcctc gaagagatct tccatttttc catcgctgtc
     6181 tttcgtgtag ctcctcgaaa cctgcccgtt ctcgtaatag taattccacg ttcccgtttc
     6241 ctttccgcaa accagtgtgc ctttgtattt cagctttccg ccggaaccgg cgtaaacggc
     6301 actgtcaata cgcggcacgc cgtaatcgta actgacgaat ttcaccgtct ttcccttcac
     6361 gaatgtttct ttgtctttcc cgtcctgcgt cacctgcagc gttttgctga tcaccctgaa
     6421 tttcacatga tcatccttga cagagataac ctcaacctcg ccgagcgaga tccagccggt
     6481 catggagctg ttgaatattt ccgtttcaaa atatttcgac atttcgccgc ggtcgccggc
     6541 gtgaatgtgg tgcgcatcgt ccgcatccat ttccggaaat ttgtcgttcg tgaccgtaag
     6601 cgttccgctg gtggaataaa gcggtgaatc ggagcatttc acgtctccgc cggactggca
     6661 ataaccaacc ggggaaaaga gaagaaatgc ggctgcggta aaaaatgcgg gagcgtattt
     6721 cattggagtt aagttttttt aaaggtagat attcattgag gaagagcatt gactttaatt
     6781 tatttctaag ctgcgccaca atgtaaaaag gcattcctgc ttccggctga acgtgtaaga
     6841 agcgcctgaa ttcaatggaa cacgaaggcc gaaacagcct atttttatac aaaaccattt
     6901 ccatgaaaac aggattgctg ttcttgttcc ttgtcatcgc agtcggtgtc ggtgcgcaga
     6961 attatcacgc gtacgttccc gggaggaccg cctttttttc acagcacctc tacaaccagg
     7021 attattccgt ggatgcgata agtccggaca cgtcatttgt ttcgggcaat tacacggtct
     7081 atcacgatta ccggcgggaa gtaacatcct cgttttcgtt ttcctgcctc accgtcaacg
     7141 acacgggctg ggcgggaaag tatttcctcc aggatgcgaa cggtgtgacg tgcttcctga
     7201 ataaagcgaa cgacagcatc tggttttttc cttcggcagc ggtggggcag gacggattta
     7261 ttttcccgtt tgccaatggc gatacgctgc acggaacggt ggtctcgctc gccattcaga
     7321 atgtgctcgg cgcacccgat tcggtgaaaa cgatgcagct gcagcggaaa gacggaagcg
     7381 gcaatagtgt ggcggatgac ctcaacggga aattcatcga ggtggcgaag gaccacggct
     7441 gcattctcgc atacagctgg agggactttc cggatgacac gacagcgcgg acgctggcgg
     7501 gaatgagcag cccgcaggaa ggcgtgcgtg tagtcccggc ggcggatgtt ttcaatttca
     7561 gcatcggcga ccagttcgac attgtagatg cgaacaacag caacgccatg caattaccga
     7621 gcatgatgtc ttactacacc tgggtggtta cggcgaaaac cgtttccgcc aacggcgaca
     7681 cgatacgcta cacaatggac gaaaactatt gccggaaaat agtcaatagc attcaggcgc
     7741 agcacatgaa cacgcccgtt atctacacgt tcattctttc ggaatacgag cgcaatttcg
     7801 aattgtcgct gatgccggga cagtacgcca atggcccgga cacggcgaac agcctttttt
     7861 acatggacgg cgcttcgttc tttccgctca atgcgacgca gtacaacgga cgcatgcaga
     7921 aaggatattc aagcaggcag gccggttatt attacgacca gaacagccag tgtttcctgt
     7981 gggacggcga ttcgcccgac atttgcgacg gcataaacga aatttatgcg gacggtatcg
     8041 gtaaagtgta cgcgcatgac ggttcggcca cttgcttcga caactaccag ctggtttatt
     8101 tttcaaaagg cgccgaaacc tggggcacgc cgaacaactg gccggtgatc ctcgggaaca
     8161 atgagctcag cgcccccgtg gtacgctgct ggcccgaccc ggcgcaggac gtgctcaacg
     8221 tggatgcggg tgcggatttc aacggcagca ttcattacca gatattggat tattccggcc
     8281 gcgtggtaag cgaggcgaac ctttctgcca gcggctccgt aatacaaatc cctgtaaatg
     8341 agctggctca cggtttttat atctttaaag ctgaaaccgg cgggcaaacg atctattccc
     8401 ggttttccca ttgagggaac agatggcaca gcgtttgccg ggataatgca aaacgcactc
     8461 agatcatgaa acgcatcctg tcatatttcc ttctgctcgg gctcgttccg cagggcggat
     8521 atgcccaatc gaccgggacg ggttccactt ccaccggcac taacgattac cgcaaggtga
     8581 cgaacaaagc gttcaagcgc ggtgaagagc tgcactaccg cgtgcattac ggcgccatca
     8641 acgccggcgt ggcggacatg acggtagaat cagacaacaa gctcatcaac aaccgcccga
     8701 cgttccacat ggtcggcgtc ggttcctcca aaggtgcatt cgactgggtt ttcaaggttc
     8761 gcgaccgcta tgaaacctac atggacgaag aagcgctgat gccctggctg ttcatccgcc
     8821 gggtggacga aggcggtttc aagatcaacc agaaccagtc gttcgatcac aacggcggca
     8881 aagtggtttc caacgggaaa tcgctgaccg ttccgaagta catacaggac atgctgtcgt
     8941 ctttctacta cgcgcgcacg atggatttca gcaaagcgaa agtgaacgat attttcagtg
     9001 tgccaacgtt cgtcgacgac gagatctggg acctgaaaat aaaattcctc ggaagagaag
     9061 cgatcgacac ggacgtcgga aaagtgaact gcctcaagtt ttgtcccgtt gtgcagaaag
     9121 gccgcgtgtt caagaaagag gaagacctta ccgtgtacat cagcgacgac gacaaccata
     9181 ttcccgtgcg tgcgcaaggc gagatcctcg tcggttccgt caagctcgac ctcatgtcgt
     9241 attcaggact ggcggcgaac ctgaatatgg cgaagtaaaa cccgggtccc ggctttgcgg
     9301 ttcgtctctt ctgcattccc tgacaatcct cattttttta cccgcgattg atttccgtaa
     9361 tttggaaaca aatcaatttt gcaatgagcg aagccggcaa caagcacaaa gacatcctcg
     9421 aaaaaataga aaacaagtat aataatctcg ggcaggatgc aggattgttc ctcaaaggat
     9481 tcagctacca gcgaccggtg acgtactggg attatgtgca gctcgaaaca ttgctctcgc
     9541 tccagcgtcc gcgcacggat tttcccgacg agccgatctt catcatgtat caccaggtga
     9601 cggaattgct gctgaagctc atcatgcacg agctgaacca ggccgtccac aacgagagcg
     9661 tgacgccgca gttcttcacg acgaaaatgc agcgcgtgaa ccggtacgcg gatatcctca
     9721 cgcagtcgtt cagcgtcatg cgcgaaggaa tggactacga gcagtacaac gaattccgcc
     9781 tcgcgcttgc accggcgagc ggtttccagt cggcccagtt ccgccagatc gagatcacct
     9841 gcaccgacgt tctccagctg ctcaatcacc gcgtgaaagg aacgatcacc gacgacagcg
     9901 atctcgataa gatattcagc gagctgtact ggaaggacgc gggccttgac cgtaagacgg
     9961 gaaagaaaac acagacgctg caattattcg aagagaaata cgagaacgac ctgaagcacc
    10021 tggccacgcg catgaaaggc aaaaatattt accggcgttt tctcgctatg gaaaaagaag
    10081 gggcgaacgt gcacgaactg aaaaacgcat tgcggaattt cgatcacacc tacaatgtga
    10141 aatggccgct ttcccatttg aaaacagcag aatattacct caactccaaa ggcgaaggta
    10201 aagctgcgac cggtggaagc gaatggcaga aatacctgca tccttcctac cagcgccgta
    10261 ttttcttccc cgcgttgtgg tcggaagacg agctggcgaa gtggggaaat ttctgatcat
    10321 ttccgcatgg gcgggtaaga cgtgcgcata aaattatttt cctgctcttt cattcttcgt
    10381 aattttcttc atcaaccgct accccgatga agagatcatt gctcttttta ttcctgcttc
    10441 ctgctgttgt tacggcgcag aactggtcgc cgctcaacct gacggacaaa tacaactacc
    10501 gtctcggcaa tgatcccgtg atcacgcaaa ccgtgtggca gaatggtttt tccgccgtcg
    10561 cgggtgatac cgttttcacg ctcaacgaca tcatgtgcga tacgtgtctc acatggacca
    10621 gcggacctct tgcgtgcgat tcctgttacg ggttgctcca cgtcgcgcag ttcgaaggaa
    10681 aacaggtgaa gcagttcgct tccggctggt gcaatttccg ggacccggga agcaaaacga
    10741 tcgatctttt cgccgcactg aacgatacgt ggctgttcga tacgctgaac aacgttaccg
    10801 cgcaggtgat cttcgcggga aattccgtgg tgttaggcaa tgcggattcc gtgaaaacac
    10861 ttttgctttc caccggcgac accttgtgtt tttccaaaga ttacggcatt gttcaatggc
    10921 cgcacgggta cgggcagaac agttattacc gcctcgcggg aatacaggga agaaatatcg
    10981 gcgtgcttat tccgcgcatg atgaattatt ttgatttcgc ggtgggcgat attttccagt
    11041 accagggaag aataaacagc ggcgcaagtc cgcaggtgcg ggatttcatc cggaaataca
    11101 tcataaccgg caaaaccgtg aacggggaca cggtgcgtta taccgtgcag gggcacatgg
    11161 cgtcgcaatg cgaggacgtt atcagcatga acttctggat ggagtattca ctgattaacg
    11221 aaacgtatac gtttatcgat tcggccaatc ataccggaaa ccagttcaac aattgcgctg
    11281 tcgatggttt cctccgcgtg atcccgaatt caggaggaat ggggcagccc cagaattcgt
    11341 tcaacccggc gcccgggtgg taccgctgca gcctgtacat ggatcctttc ggcaataaag
    11401 gaatacgttt cggaacggcg acttcataca ctgatttcca tattgatcct tcgtgttccg
    11461 ttcctgtttt tgcaggttca gatacgctcc aaccggtttc ctggggggat gcgttcaccg
    11521 ttcagctcac ggaaggactg gggcagacgt tcgctttgtg gacgtccaat ttcgaaggat
    11581 catggtcgga gcagctcacg gctttccgga agaacggcga tacgaccggt gtgctcatac
    11641 ccgacaacca gctcgatgct cttgcacaac atcccggtag cggaggcgga atgacggttt
    11701 atcccaatcc ggcggatgaa gcgctgatca tttcactgcc cggaaatgtg caggcagatc
    11761 ttgtgatcac cgatgcaggg ggccgcgttg ttttccggca gcagaacgca gcagggaata
    11821 ttcagctggc gacggcggat ctcgcggagg gcatgtattt tctccgggtg ataacggcgc
    11881 agggcgcagc ggcggagaag atcgttatcc ggcattagca ccttttgaag tctgcagcgc
    11941 gcaactgatt tccgggaacg accgttcgct ttttttaatc gttccaccct gtgattttct
    12001 aaatttgtga cgaacgtctt acagcaaggc cgccccggct tgttctttgc gtcatatccg
    12061 cagcaacgta atgtttcgca taactcccgt agcgattttg attttcatgc tttgcccggc
    12121 tttgcatgcc gttacgctga gcgggaaagt cacgtctccg gccggtgaag cgctgggata
    12181 tgcggctgtt tacgtgaagg gaacgtcgaa aggaacgacg acgaacacgg aaggcgatta
    12241 cacgctcgat cttacgccgg gaaattacac gatcatcttc cagatgatgg gttattacca
    12301 gaagcagttc gacgttgatc ttacacaggg aaacaaaacg cttgatgttg ttctcacacc
    12361 caccacgctg cagctgaagg aagccgtgat cacggacggg gaagatcctg cgtacgctgt
    12421 gatccggaac gcgatcagga aacggaaata ttaccgcgac caggtggatg cgtatacgtg
    12481 cgacgtctac atcaaaggat tgcagcgcgt cacgcagcat ccgcagaaga ttatgggcat
    12541 ggacgtcgac ccggatggcg acatcgattc cgcctcgggg atcgtttatc tctcggaatc
    12601 cgtttccaca ttcaatttca ggcagcccga caagatcaag gaggaaatgg tttcctcgaa
    12661 agtaagcggc gacaacaaag ccttcagcta caaccgcgcg tcggacatgc tgttcaattt
    12721 ttacgacaac gtgatcgaga tcgaagatct cagcgaacgc ggtttcgtca gtcccatcgc
    12781 gaacaatgcg cttttcttct acaagttccg gatggcggga acgatcatgg ataacgattc
    12841 gatggtgaac aagatcgagg tgatcccgaa acggaaaacg gatccgtgct tccacggcta
    12901 catctacatc atggaaaatt cctggcgcat ttacgcaacg gacctttacc tgacgaaaga
    12961 cgcgcagatc gatttcgtgg atacgctgac gatcagccag acgtatattc ccgtggaaaa
    13021 ggacgtgtgg atggtgttca tgaacaagtt cacgttcgtc ttcggcttca tgggcctgca
    13081 gggaaacggt tattacgtgg gtgtgaacaa ggattacgtc atcaatcccg atttcccgaa
    13141 aaagtttttc gataacgaag aaatgcacgt cgccgatgat gcgaacctga aagattccgc
    13201 ttactggcag acgacgcggc ccgttccgct gacagacgag gaagagcgcg attacaaacg
    13261 gcgggacagc ctgatgcaga tacggaattc gaaaccgtat ctcgattcca ttgacaaaag
    13321 aaccaaccgg cccgggttca ccgatatttt tctgaccggc tattcgtatt cacggcggta
    13381 caatcatttc agcgtttccg tttcaccggt cgtgcgtgcg ttcagctaca ataccgtgca
    13441 gggctggcgc acgggcctcg acgtggacgc gttcaagagc tgggacaaca accggtggct
    13501 ttccgtcggg ccttcggctt cgtacggcat cggcgataaa aaattctatg tcgacggtga
    13561 agcgacctat tactacaaac ctgtccggtt tgcgcgtgtc accgctgccg gcggaacgac
    13621 aacggcgcag ttcagtccgc gcgatccggt tcctgttttc atcaacacga tctacacgct
    13681 cggtgaaaaa cagaattaca tgaaactgta cgcgaaaaaa tacggcagcc tcaactggtt
    13741 cggtgaagtg acgaacggtg tgatgatgaa catcaatctc gaatacgccg accgcacacc
    13801 gctttacaac acgtcgttct attactggaa ctggcgtttc ggtccgtcca gcctgccggc
    13861 gcccggctac ctggaaacgc gccgtttcac gtcgaacaac ccgctcgatc cttcggataa
    13921 cagcagtgcc gctttcggcg cgcaccgttc gctcgacctc aacgcgcagg cgatcatccg
    13981 gttcaaacag aaatactaca cgcgcccgaa tgagaagatc attctcgggt cgaaatatcc
    14041 tgccatcggc atccagtacc gccgcggcat cccgggtatt ttcgggagca aaacggatta
    14101 tgattttctc aagctgagcg ttcgcgacga gatcaacctc aagctcatgg gaaaattccg
    14161 ctattatttc gccgttggaa aattcctttc gcagaaatac ctgccgttca tggattatta
    14221 tcatttccag ggcaaccgca cattctggtc gacattcgcg cagcagagtt tccagctgct
    14281 ggactattac acgtattcca cgaacacctg gttcatggaa gcgcatgccg aacatcattt
    14341 caacggcttc ctcttcgata aaatccccct gctgaaaaaa atgcacctca cggagatcgt
    14401 cggcgtgaac tggttcatga cggaccgcgt gccttcgtat tatgagattt atttcggcgc
    14461 cgacaagctc ggcctcatcc gcgtcgattt tgcgctgggc tatatgcaga atgcgaaagt
    14521 gagcgcagga ttacgattag gaatacgaat ttgataaact gcaactatga acaccatgaa
    14581 taaccgcgtc attcccggaa gccggaagct ggtcctgttt ccattgctgc tgctgatttc
    14641 catcggcgcg cacgccttcg tttaccagac gttcgacaaa acgctttcga acgggctgaa
    14701 agtgatcacc tgcgaaaagc cgggaagcgg ttacgcggaa gtggaggtgt ggtaccgcgt
    14761 cggcagcaag gacgaacacg cggggatccg cggcatggcg cacatgttcg agcacatgat
    14821 gttccgcggg acgaagaatg tttccggcga gcagctgctc gaaaagctcg acagctcggg
    14881 cgcgcagtgg aacgcgtaca cgacgttcga ccgcacggtt taccacgagt atcttcccgt
    14941 gaacgccgta agcatggcgc tttaccttga ggcggaccgc atgtcgaacc tcgtagtgac
    15001 gcaggaaatt ctcaacaccg aacgcgaggt ggtgggcgaa gaataccgca acggcatgag
    15061 caactggtat gaaaaaatgg tggaggaccg ttacgaatat ctttaccccg acggtcatcc
    15121 gtaccagtac gacgtcatcg gtcacctcga cgagatcacg aatttcaccg cgccggaatg
    15181 catggacttt tacgacaaat attacagtcc caacaacgcg ttcgtcgtcg tggtgggcga
    15241 cgtgaagcac gacgatattt ttgcgcaatg cgaaaaatat ttcgggccca tcaccaagca
    15301 attgcccgcg gtcgttccgg gcccttctcc ggatatcatg ggcacgcacg tgaaaacgac
    15361 ggacatggag ctcaacttcc cgctgcagat ctattgctat tcattgtcgc agccggcagc
    15421 cggctcgaag gattttttcg cgttcaacat gctggcggat atttttttca cgggcgataa
    15481 ttccatcctt aacgaacgcc tggtgaaaaa agagcacagc gtttatttca tcaaccagtc
    15541 taacgaagcg tggagcctgt acccgtcgcg gacgcagttc gacgtgttca tgccgccctc
    15601 gccgggaaat gtgaaagtga agaaggcgat cagggccgag atggacagcg tcattgcgaa
    15661 cgggctgccg caggaaaagg tgaacgattt tatttcgaac atggaagcgc agcagacgct
    15721 gggtgcgtat tcgtctgaaa acatcgcggg cgagctcggc atggcggaat attatttcca
    15781 cgattacaac cgcgcgaaaa ccattgtcaa tgattacaag gccgtcacgc aggatgacat
    15841 caaacgcgtc gccgccactt atttttcacc cgatcatgtc gacgtgatca acatcaaacc
    15901 ttcgttctga tatgaaaaag agatcgcttt tccgtctcac gctggttccc gttgcattgc
    15961 tgctgctcgc ttccgctgcg caggataaag gaccgttccc cgatatcgag cggtacaagc
    16021 tgaagaacgg gctcgaagtt attttcgccg attacggcga tcttcccgtg acgtcgctca
    16081 cgtttttcgt caacgtcggg aagaagagcg aaacgcccgg ccagcaggga cttgccggcc
    16141 tcaccgcgaa ttcgctgttg ctcggcaacg acaaattttc acgcacggac caggaccgga
    16201 tgctgtaccg cctcgggacc gggatcagcg ctgatgcgaa tgaaaatttc actaccgtca
    16261 gcggggaatt cctcaatacg gacatcggca agggattcga aattgtttcc gccgtgctgc
    16321 ttcatcccac cttcccgcag caggacatcg acgaagaaaa aaatctcgac atctcgcaga
    16381 acaagccttc gaaaatggac ataggcgatc tcgcttcgat gtacggcaat tatttctgct
    16441 acgggatcgg tcacccgctc gggcggcatt tttacgaagc acagtacaag aagatcacga
    16501 cggcgcagat cagggatttt tacaatttca actatacgcc gggcaacacg aagctcgtcg
    16561 tatgcggaaa acccgaccgc gaaaaagtga aagcgctcat tgagcagtat ttcggcggat
    16621 ggaacgccac gtacggagaa gtgaacggct cgtcgtatga cataccgccg atcaaaacaa
    16681 gggaattcgc attcgttccg aaagacagcg cgaagcaggc ctgcctgctg tggatgaaaa
    16741 aaggaccggc cgcgggaagc aaggacatga cggcgttcat catcgccaat gccgtttttt
    16801 cggatcacct cacgaaagag atacgcgaaa agcgcggcta tacgtacggg atctacagca
    16861 cgttcagcca ggcgcaggac gacgggattt tccgtgcacg cacgcaggta aggaatgacg
    16921 tgatgttcgc aacgatgacg gcattcgaca atgtgctgac ggaattttat gcgaaa
//
LOCUS       DASTSF010000313         6183 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_548094,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000313 DASTSF010000000
VERSION     DASTSF010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 6183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6183
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_548094"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..810
                     /locus_tag="VFU15_08990"
     CDS             <1..810
                     /locus_tag="VFU15_08990"
                     /inference="COORDINATES: protein motif:HMM:NF020387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES domain-containing protein"
                     /protein_id="HEU4717959.1"
                     /translation="EAICLAEYLDKAIDFSLLARNDMSFDRIYRITMADDSFREKGKI
                     RDVKYLKYPPVELVKKQKRYNRANTFNKTVFYAASNWHIALLETKPHPGKRIIMSVWE
                     PLTDELFNSYPISNVQVDNQSVRDATAYLKEYGKSVHPLCAEAMDLILEFLASEFVKD
                     VQAVNPNRLEYFYSAYFSDIILGPIIADDPTATCDLILYPSVAWNHKLDNIAMSPYCL
                     DEKMRITHVVEFEVEETYYEQKLPLEQCPAKLRFIREATWFDGDLIVWEDE"
     gene            complement(1092..1457)
                     /gene="ssrA"
                     /locus_tag="VFU15_08995"
     tmRNA           complement(1092..1457)
                     /gene="ssrA"
                     /locus_tag="VFU15_08995"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            1777..2973
                     /locus_tag="VFU15_09000"
     CDS             1777..2973
                     /locus_tag="VFU15_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002692992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU4717960.1"
                     /translation="MKIGILREGKMPRDKRVPFTPAQCVLIQREFNVDVLVQPSEWRS
                     FSDEEYRAAGITLSEDLSSCDVLLGIKEVPVDDLIPGKKYLFFSHTIKKQPYNQKLMH
                     ALIAKKVRMIDYECLTDPQFNRIIGFGHFAGIVGAYNGLRGYGLRFGFFDLKPAWKCH
                     DMTELKEELKKVKLPNLKMIVTGNGRVANGAIELLGLLHIRRVTPYEFTHYSFREAVY
                     TQLHSYDYNEPKNGLQWDRDRFYHHPQEFRSTFAKFTPHCDLLIHCSFWDPRAPKLFE
                     KQDMRSPEFRISVIADVTCDIDGSIPSTTKPSSIAEPFYGYNPNTEQAVDKPFGNDVI
                     TVMAVDNLPCELPRDASENFGKELIEKVVPALLGNDPDGLIERGSICSDGKLTERFGY
                     LADYAL"
     gene            complement(3099..4448)
                     /locus_tag="VFU15_09005"
     CDS             complement(3099..4448)
                     /locus_tag="VFU15_09005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717961.1"
                     /translation="MRSLLISLALLLPFAAANADEDSLTVEKAFVHQAGRNNLYVLQL
                     KKGGQYDYTRYTDKKLYHDFGVFRIRHGKISFESKSQKRTFASVGGKTYFMNKKGLYK
                     KRMDEIRDKKSAMKFTDDPSYKKSWSFNPVAGRVMEPDGKPVVKPAAPKTNTDDVATK
                     NKKITDYTEAFYVSMAGRFASPYKALLETDYTGPDTFFKLQNNQPVTWNYDTTKSALQ
                     DDFETIIHESTHTFIHTNAGGGNFSYLVQPGLSVNVTPTPAFHSSEIKALAPADATKR
                     IFRYGTYVSDSSVVTANLDGIYGLMDEFTAYENGTRSSVIAAQNAMQQGDTALASSLV
                     SQAGGTYFAYYEFNLFMAWYLHEAKLKHPDDYKAIMANTNLRVAYTLVDAEFAQTITD
                     LKRTSAIVQKKTGTDYLAYNEKTYAAYPKELLVKEKTYLDAFRVKGVTNSNYFTFVN"
     gene            complement(4677..>6183)
                     /locus_tag="VFU15_09010"
     CDS             complement(4677..>6183)
                     /locus_tag="VFU15_09010"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEU4717962.1"
                     /translation="ALWFLGNPSEVFHTASWKQLIFGILVTGILSALIYVFYKKFISP
                     RKPPEPRPFYFTAIYSLALPVLLAIGVRGGLQPIPIQVSDAYYSRFNVLNLASVNSTF
                     NLFSSCIENIHADKAYTFMPSPEAAKVFSEMNAVTKDTTLHVLTTDKPNVVLVVLESW
                     SADLVKSCGGYDSITPEFEKLASQGILFTNCYASGSLSDQGMAAVFSAFPAQPKTSVI
                     TQPTKYVHLPCINNSFRQAGYKTSFMFGGQLSYGNIRAYMYYNNFDRIIEGKDFDPSV
                     PQGRLGAADQYLFSRQLQELSKEQQPFFAAMFTLSTHGPFDFPMEEKLHWGDKEKPYI
                     NSVLYADGCIGNFISEAKKYPWYSNTLFIFVSDHSHNSPKNWAFNDPRYRHIPLLFYG
                     DVIKPEYRGMKYDSVASQTDLASTLLHQLDMDASAFRYSKNLFNPYTPHYAYYAFDEG
                     FGLVQGGSHLTWSVKDNRYEFEQYASPADKNKLATDGKAFLQELTEDYFRF"
BASE COUNT         1439 a         1547 c         1590 g         1607 t
ORIGIN      
        1 gaggcaatat gtctggctga gtatctcgat aaagcaattg actttagtct gctggctagg
       61 aatgatatgt cttttgacag gatatatcgt attacaatgg ccgatgattc atttagggag
      121 aaaggaaaga ttcgggatgt aaaatatctt aagtacccgc ctgtagaatt agtgaagaaa
      181 cagaagagat acaatcgagc caataccttt aacaaaactg ttttctacgc agcctcaaat
      241 tggcatattg ctttgcttga gacaaaaccc catcctggga aaaggattat tatgtcggta
      301 tgggaacctt tgactgatga gctatttaat tcctatccga tatcaaatgt tcaggtagat
      361 aaccaaagcg ttcgtgatgc aacagcatat ttgaaggaat atggtaagtc tgttcatccg
      421 ctttgtgctg aagcaatgga tttaattctt gagttcctgg catcggaatt tgtcaaagat
      481 gttcaagctg tcaaccccaa caggttagaa tatttctata gtgcatattt ttccgatatt
      541 attttgggac ctattatagc tgatgaccct actgcgacgt gtgatcttat tctttatcca
      601 agtgtggcct ggaatcataa actcgataac attgcaatga gcccttattg tctggatgaa
      661 aagatgcgaa ttacgcatgt tgtcgaattc gaggtggagg aaacatatta tgaacaaaaa
      721 ttacccttag aacagtgtcc ggctaaactg cgattcattc gtgaagctac ttggtttgat
      781 ggcgatctga ttgtttggga ggatgagtag cctggatgtc atgctttaat cagtaactgc
      841 ctatatttct tttcatcttt ccaaagtgca agttttatca tgcaactgat cgttcaataa
      901 actctttccg tacgatctaa acacgcgagt tcgacttttg agttcgatcc ttcccgctaa
      961 aatttcgttt tctgaagaaa aatgtcgaac tccacgactg aacgaggtgc tccgccgctg
     1021 tctcaatatt gacaagggtt tcagccaaaa tgaaaataga caacccctga aaaatctgga
     1081 gttgtctact ttggtggacc cggagagaat cgaactctcg tccaggcaag ccgtgaatgc
     1141 gctttctaca tgcttagccg gttattgttt gtcgggaaaa accaggttaa cctgcacacc
     1201 gaatttttcc gtagttgctg atagtgcttc ggcgtcgcaa cgccaccggg cactcctttt
     1261 gtttgtggtg ttccggatcg ggacgtcaga aggaaaaact tcccggggaa cattgctgaa
     1321 taattcttgg aattaagcag caagagcgaa gttagattcg ccgtttacaa gtttgagagt
     1381 tgatattaac gtgccatatt cccaacgcac ggcatgctta cacacccgcg cacatgctgt
     1441 caaaaccggt cgggcccgtg taatcttgca caaagatact gcgaattaca cccgcttgtc
     1501 aaatgaattt cctcccgcac cggttgacgt tccccgcttt tcattcttgt ttgcagggcg
     1561 tgcccccatg caggaacacg atgtgtttcc tccatggggt ccggctgtcc gctctatctt
     1621 ttgcttcgca aaaggatgcc gcttccatcc gtcacgcaga aaaaaaatga atgttgaacg
     1681 atgcatgatc ggcagggaat gacgggcagc aaaccgctgc cggcgatcgc cagtcaatga
     1741 acaactgcct atctttgcct ctcaaatttt ttgcttatga aaatcggtat cctgcgcgaa
     1801 ggaaaaatgc cccgcgacaa acgtgttccc ttcacgcccg cccagtgcgt gctcatccag
     1861 cgcgagttca atgtcgatgt gcttgtgcag cccagcgagt ggcgcagctt ttccgatgaa
     1921 gaataccgcg ccgcgggcat cacgctctcc gaagatcttt cttcctgcga cgtgcttctc
     1981 ggcatcaagg aagtgcctgt cgacgatctt attcccggga aaaaatatct tttcttctcg
     2041 cacaccatca agaagcagcc gtacaaccaa aagctcatgc atgcgctcat cgcgaagaag
     2101 gtccggatga tcgattacga atgcctcacc gatccgcagt tcaaccgcat catcggtttc
     2161 ggtcatttcg cgggcatcgt cggtgcgtac aacgggctgc gcggctacgg actgcgtttc
     2221 ggctttttcg atctcaagcc ggcctggaaa tgccacgaca tgaccgagct gaaagaagag
     2281 ctgaagaaag tgaagctgcc gaacctgaag atgatcgtca ccggcaacgg gcgcgtcgcc
     2341 aacggcgcca tcgaactgct cggcctgctg cacatccgcc gcgtcacacc gtacgagttt
     2401 acgcattatt ccttccgcga agctgtttac acgcagctgc attcgtatga ttacaacgag
     2461 ccgaagaacg gcctgcagtg ggaccgcgac cgcttctatc atcacccgca ggaattccgc
     2521 agcacgttcg ccaaattcac gccgcactgc gacctgctta ttcactgttc gttctgggat
     2581 ccgcgcgcgc cgaagctgtt cgagaagcaa gacatgcgtt cgccggaatt ccgcatcagc
     2641 gttatcgccg atgtcacctg cgatatcgac ggttccattc cttccaccac caaaccttca
     2701 tccatcgcgg agccgttcta cggttacaac ccgaacacgg agcaggcggt cgacaagcct
     2761 ttcggcaacg acgtcatcac cgtcatggcc gtcgataatc ttccctgcga gctgccccgc
     2821 gacgcatctg aaaatttcgg aaaagagctc atcgaaaaag tggtccccgc tctcctcggg
     2881 aacgatcccg acggactcat cgaacgcggc agcatctgca gcgacggaaa gctcacggag
     2941 cggttcgggt atttggcgga ttacgcgctg taaaacgggg aatggagaag gagggaggaa
     3001 ctctttcttt atccacacac actttcattt ttcctccctc cgttcttttc ttcacttgtc
     3061 caactcaaaa cgcgcaaaac ccgtagcccg taattttttc aattgacaaa cgtgaagtag
     3121 tttgagttcg tcacaccttt cacgcggaag gcatcgaggt acgttttctc tttgacgagc
     3181 aattctttcg ggtaagcggc atacgttttt tcattgtacg ccaggtaatc ggttcctgtt
     3241 ttcttctgca cgatcgccga tgtgcgcttc aggtcggtga tggtctgcgc aaactctgca
     3301 tccacgagcg tgtacgccac gcgcaggttg gtgttcgcca tgatcgcctt gtagtcatcc
     3361 gggtgcttca gcttcgcttc gtgcaggtac cacgccatga acaggttgaa ttcgtaatac
     3421 gcgaaatacg ttccgcccgc ctgggaaacg agagaggagg cgagtgcggt atcaccctgc
     3481 tgcatggcgt tctgcgccgc gatcacgctg ctcctcgtcc cgttctcata agcggtaaat
     3541 tcatccatca gtccgtagat gccgtcgaga ttcgccgtca ccacgctgct gtcgctgacg
     3601 tatgttccat agcggaaaat gcgtttcgtc gcatctgccg gcgccagcgc ttttatttcc
     3661 gaagaatgga atgcgggggt cggcgttacg ttcaccgata ggcccggctg cacgaggtag
     3721 ctgaaatttc cgccgcccgc attcgtgtga atgaacgtgt gcgtcgattc gtggatgatc
     3781 gtttcgaaat catcctgcag cgcgctcttc gtcgtgtcgt aattccaggt caccggctgg
     3841 ttgttctgca gcttgaaaaa tgtgtccggg cctgtgtaat ccgtttcgag cagcgccttg
     3901 tacggcgaag cgaaccgccc ggccatgctc acgtaaaaag cttcggtata gtcggtgatc
     3961 tttttgtttt tcgtcgctac gtcgtctgtg tttgttttgg gcgccgcagg ttttacaact
     4021 ggttttccgt cgggttccat cacgcgtcct gcaacggggt tgaagctcca gcttttttta
     4081 tacgacggat catcggtgaa cttcatcgcg gattttttgt cgcggatctc gtccatcctt
     4141 tttttgtaaa gacctttttt gttcatgaaa tatgtctttc cgcccaccga tgcgaacgtg
     4201 cgtttctggc tcttcgattc gaagctgatc tttccgtggc ggatccggaa aacgccgaaa
     4261 tcgtggtaaa gctttttgtc cgtatagcgc gtgtaatcgt actgcccgcc ttttttcagc
     4321 tgcagcacat acaggttgtt ccttccggcc tggtgaacga aggccttttc tacagtcagg
     4381 gaatcttcat cggcattcgc cgcggcgaag ggaagaagca gcgcaagaga aatgagcagg
     4441 gatcgcatac acagccgttt tttggtttga gtaacgtgat gtcatttccg ttggttcttg
     4501 tttaattaaa acgtggatcg cttcccggtt attgtgcaac acgcgtgcca tgtttgattt
     4561 tgactttccg cggagttgca ggtgaacggc tgaaaacggt cccgaaagtt ccggaagaga
     4621 aaagaaatga tcagcggatg aattggaaaa cggaagtggg tactaatgaa gaaaattcag
     4681 aacctgaaat aatcttccgt cagctcctgc aggaatgctt tcccgtccgt agcgagcttg
     4741 ttcttatcgg ccggcgatgc atactgctcg aattcatagc ggttgtcttt cacgctccac
     4801 gtgagatgtg atccaccctg cacgagcccg aatccttcgt cgaacgcata gtaagcgtaa
     4861 tgcggcgtat acgggttgaa aaggtttttg ctgtagcgga aggccgacgc gtccatgtcc
     4921 agctggtgca gcagggtgga agcgaggtcg gtttgcgatg ctacgctgtc gtatttcatg
     4981 ccgcggtatt ctggtttgat cacgtcgccg taaaacagca ggggaatgtg acggtagcgc
     5041 ggatcgttga aggcccagtt cttcggtgag ttatgcgaat ggtcggaaac gaaaatgaaa
     5101 agcgtgttgc tgtaccaggg atattttttc gcttccgaaa tgaaattccc gatgcagccg
     5161 tcggcgtaaa ggaccgaatt gatgtacggt ttttccttgt cgccccagtg cagcttttct
     5221 tccatcggga aatcgaacgg gccgtgcgtg ctcagcgtga acatggcggc aaagaacggc
     5281 tgctgttctt tcgacaattc ctgcaattgc cgtgaaaaaa gatattgatc agctgcgccg
     5341 aggcgtccct gcggaacgga aggatcgaaa tcttttcctt cgatgatgcg gtcgaagtta
     5401 ttgtaataca tgtacgcccg gatattgccg tagctcagct gcccgccgaa catgaacgag
     5461 gttttgtaac cggcctgccg gaatgaatta ttgatgcacg ggaggtgaac gtattttgtc
     5521 ggctgcgtga tgacggatgt tttgggctgc gccgggaaag cggagaacac cgccgccatt
     5581 ccctggtcgg aaagacttcc gcttgcgtag cagttggtga aaagaattcc ctgcgacgcc
     5641 agcttttcga attcgggcgt aatgctgtcg tacccgccgc agcttttcac gaggtcagcc
     5701 gaccagcttt ccagcaccac gagcacgacg ttcggtttgt ccgtggtcag cacatgaagt
     5761 gtcgtgtctt tggtcacggc gttcatttcg ctgaaaactt tcgcagcttc cggcgacggc
     5821 atgaacgtgt acgctttgtc ggcatgaatg ttttcaatgc agctgctgaa caggttgaac
     5881 gtggaattca cggaagcgag gttcagcacg ttgaagcgcg agtaatacgc atcgctcacc
     5941 tgtatcggga tcggctgcag tccgccgcgt acaccgatcg cgagaagaac gggaagcgcc
     6001 agcgaataga tggctgtaaa ataaaacggt cgcggctccg gcggtttccg gggtgaaatg
     6061 aattttttgt agaaaacata aatgagcgcg gaaagaattc ccgttacaag gatcccgaaa
     6121 atgagctgct tccacgaagc ggtatgaaag acttccgacg ggttgccgag gaaccagagc
     6181 gct
//
LOCUS       DASTSF010000314         7332 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1420026,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000314 DASTSF010000000
VERSION     DASTSF010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 7332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7332
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1420026"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            9..1031
                     /gene="fbp"
                     /locus_tag="VFU15_09015"
     CDS             9..1031
                     /gene="fbp"
                     /locus_tag="VFU15_09015"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015808778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HEU4717963.1"
                     /translation="MSKVKTLGQFIIEKQSDFPFAKGELSRLLRDIGIAAKIVNREVN
                     KAGLADILGEAGETNVQGETVKKLDVFANEQFIAALSVGGECCAIASEENEEIIPINT
                     PQSKNAKYVVAIDPLDGSSNIDVNVSVGTIFSIYRRISLSGPGTIEDFLQRGTEQVAA
                     GYIIYGSSTMLVYTTGKGVNGFTLDPSIGEFCLSHPNMKTPVEGKTYSINEGNYVHFP
                     DGVKKYIKYCQEEDKPTKRPYSSRYVGSAVADIHRNLITGGVYIYPTTASSPKGKLRL
                     LYECNPFAFIIEQAGGKATDGFSRILELEATSLHQRTPFFAGSANMVDKSGEFMLKHS
                     SVASEK"
     gene            1153..1497
                     /locus_tag="VFU15_09020"
     CDS             1153..1497
                     /locus_tag="VFU15_09020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717964.1"
                     /translation="MKTLRIFTALFLLAGFLPLQAQDKNSNDSTDLIYKKIVRSVSVP
                     DQLRNENVDLERVRVVFTIDEKGRAHVIDVNTKRPDIIASVTKQFEAIDFSGSTGTDG
                     KTYSIWLNFKVV"
     gene            1559..2995
                     /locus_tag="VFU15_09025"
     CDS             1559..2995
                     /locus_tag="VFU15_09025"
                     /inference="COORDINATES: protein motif:HMM:NF022441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HEU4717965.1"
                     /translation="MEKIIMKKMALVIAGMAGMIFLHAQTPSETRTVDDFTGIKITAP
                     VSLQLTQGDNNAVVVETNQEALKDITTEVKDGILVIGATTMKNVSDEAVVKVTVKNLT
                     SLDAGSTSNVTTTNQLVADNLAITASDASVADLDVKAGNVKIVLSGAAKTKLSGTATS
                     LDATLSGASQLKAYSMQAEKATITASGASVAHVSVTSAIMATASGAAEVHYQGNPVEK
                     NIDASGAASVGVKGGDSDTASFHISGKDVYVVDNDDDDERSPREKKADDKDFEFWSGL
                     DFGVNGYLTADNKLDLPQASSFMNLNYAKSYMFGWNIWQKNIHIYRNNVNLGTGIGLN
                     WYHYNFRESYSLQPDLPFTTAVADSLNFTKNRLNMCYVGIPLFLEFNTNNSDARNSFH
                     IGAGVNAGYNVFRNKLKQRYDYAGRVYKRKVVNDFNVDPFRIDAMLRIGYGNYTIFGA
                     YSLTTLFEKSKGPTVYPFSAGIHVDFGH"
     gene            3097..4401
                     /locus_tag="VFU15_09030"
     CDS             3097..4401
                     /locus_tag="VFU15_09030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717966.1"
                     /translation="MKRSRCLFLFFIAFIAALPAQQLVKKKIVYDSPGILKKGMNAHE
                     FFFLPFNVSDTTSDETLLSAMDSVPPLITQVVYGTDAKDTTKYNVIIAGSRLSGTYSV
                     TRVYPDGCFSGTHTYGRNHRLTGGYATYYPGNHIKSAGQYMDGKKQGRWRLYDENGRL
                     LWKGKYRNDSLVKAKDYGHSRFTIYSLYRHRDLRPYNYVIVYGKDTAADYFPEDTANV
                     SRHLPFGMNCSADMYTMDFHNARNSMPQLGGYPNDYCIAMGDEIYAGYREKFFVTAGL
                     HFNLPKEFTGNYPGYGKVAVEAAQFRAHLDGNLPVIRNPKRTLFVYGGIMFARTYLTS
                     LQRYDNGNSVSYFETSHIKQSLADAGLLFECTARPFTPRYTGYFSIKAGVNYPIGNAQ
                     WYYFFSPQVTYPESGKPGVKLGVFYFSVGFTIWKSDHRRTSP"
     gene            4409..4948
                     /locus_tag="VFU15_09035"
     CDS             4409..4948
                     /locus_tag="VFU15_09035"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU4717967.1"
                     /translation="MNTILPVPGGIYLSPPGEKDIPAFVKCLNDREIFDRTLMIPFPY
                     TETDALNFLAFAHDARSRYGHELNFVIRDANGEMMGGAGYHGKNFHSTNAHRDELGYW
                     VAEPFRNKRVMSRVLPVLVQYGIEVRRLKRIEACIYAFNDASERLLLKNGFVMEGILR
                     KAYYKNAQFFDAKLFSLIV"
     gene            5206..>7332
                     /locus_tag="VFU15_09040"
     CDS             5206..>7332
                     /locus_tag="VFU15_09040"
                     /inference="COORDINATES: protein motif:HMM:NF026255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEU4717968.1"
                     /translation="MPVSWKVVFFFVLVQTAAHAQGSVSGKIVDSTQAPVPFVVVALL
                     HPGDSAIRAGTLTDESGHFVFRNVRTGKYFLKISGGAYEEQFSDVITFDSIHDLTVPD
                     IVLRRSKVFDAVTVTAVKQTVVFRNGNTIVNVGNSALAKGNSVFDLLSKLPGVTVDNN
                     IISLQGKSGVIVMIDNRVQQVSNDQLINLLKSMSAESVERIEILKNPPVKYDASGTSG
                     MISIITKKNTQKGFSGNVYATTSQGFYNNSDAGLSLDYRNDRFSFFSGIDADDGWYRS
                     HEKFYRNFVTGSGVTQMVNENFTRERGSDLNFRIGADWSVSPRTTLGAKIDGGPGAYT
                     SYETGSDQVFDYNSTGFDHLVSGNYIPNVWNTVNYNVNGERLFDTLGTALNFSSDFTD
                     LRETDEGHGWNYFYDANGNNALPPDINQSHNRSATQIFSARLDFAKKRDTASSFEAGL
                     KASAVTTGNDYLFERQDNFTGNFYSDTAFTSNYIYSEQTYAGYANYSRAWKSFSMELG
                     MRAEETALSGHNPHSGFAMADHYFNVFPDISLDFSKSEDHDFQLNLNRRIDRPDFDEL
                     NPFIYYRDPFSYFRGNQFLRPQYSDRAELTYSYQGAIVNSVSYERLDKFVLDYTVQND
                     STKIFLETEKNVSSATECTYSLFVQEDVFPWWSVTATGNFLLLDFRGDVQGVPFHTRG
                     SSYYANVMNTLIFPHKTNLEVMFMYRG"
BASE COUNT         1758 a         2003 c         1915 g         1656 t
ORIGIN      
        1 cattcctcat gagcaaggtc aaaacactcg ggcagttcat catcgagaaa caatcggatt
       61 tcccttttgc caagggagag ctttcccgcc tgctgcgcga tatcggcatc gccgcgaaga
      121 tcgtcaaccg cgaagtgaac aaggccgggc tcgccgacat tctcggcgaa gccggtgaaa
      181 cgaacgtgca gggcgaaacg gtgaaaaagc tcgacgtgtt cgcgaatgaa cagttcattg
      241 ccgcgctcag cgtaggcggt gaatgctgcg cgatcgcttc cgaagagaat gaagagatca
      301 ttcccatcaa tacgccgcag tcgaagaacg cgaaatatgt cgtcgccatc gatccgctcg
      361 acggctcatc gaacatcgac gtgaacgttt ccgtcgggac gatcttttcc atttaccgcc
      421 gcatttccct ctcgggcccg ggaaccatcg aagattttct ccagcgcggg accgagcagg
      481 tcgctgccgg ctacattatt tacggatcat ccacgatgct cgtgtacacg acgggcaaag
      541 gcgtcaacgg ttttacgctg gatccttcca tcggtgaatt ctgcctttcg cacccgaaca
      601 tgaaaacgcc ggtcgaaggg aaaacctatt cgatcaacga aggcaactac gttcattttc
      661 ccgacggcgt gaagaaatac atcaaatact gccaggaaga ggacaagccg acgaagcgtc
      721 cttattcgtc gcgttacgtc ggttcggccg tggcggacat tcaccgcaac ctcatcaccg
      781 gcggcgttta tatttatccc actaccgctt catcgccgaa agggaagctg cgccttctct
      841 acgaatgcaa tccctttgcg ttcatcatcg agcaggccgg cggaaaagcg acggacggtt
      901 tttcacgcat cctcgagctg gaggcgacct cgcttcacca gcgtacgcct ttcttcgcag
      961 gctcggcgaa catggtggac aagtcgggcg aattcatgct gaagcattcg tccgtggcga
     1021 gcgaaaaata agcgcgggtc ccttttttgt tttcgttaaa aaaagaaaat gatgcagctg
     1081 ttttttttca gctgtaacat ctgcgcgcct gtgtgcgtca catgcatgaa gaatcaaaaa
     1141 cacatcctca tcatgaaaac gctcagaatt tttaccgcct tatttttgct cgctggtttt
     1201 cttccgcttc aggcgcagga caaaaacagc aatgacagca ccgacctgat ctacaaaaag
     1261 atcgtccgct cggtgagtgt tcccgaccag ctccggaacg agaacgtgga tctcgaacgc
     1321 gtgcgcgtcg tcttcaccat cgatgagaaa ggccgtgcgc atgtcatcga cgtgaatacg
     1381 aaacgcccgg atatcatcgc ttccgtgacg aagcagttcg aggccatcga tttttcgggc
     1441 tccaccggga cagatggtaa aacatactct atctggctga atttcaaggt agtataatct
     1501 cttcagcaac agcctgtaac tatccttgcg acattcgcgt ctcacaacaa acattaacgt
     1561 ggaaaagatc attatgaaaa agatggccct ggttatcgcg ggaatggcgg gaatgatttt
     1621 cctgcacgcg cagacacctt ccgaaacaag aaccgtagat gattttacgg gaataaagat
     1681 cacggcgccc gtgtcgctcc agctcacgca gggagacaac aacgcggtgg tggtggaaac
     1741 caaccaggaa gcgctgaaag acatcacgac cgaagtgaaa gacgggatcc tcgtcatcgg
     1801 tgcaacgacg atgaaaaatg tcagcgatga agccgtggtg aaagtgacgg tgaaaaatct
     1861 cacctcgctc gatgccggtt ccacttccaa tgtcacaacg acgaaccagc tggtggcgga
     1921 caaccttgcc attactgctt ccgatgcatc ggtcgctgac ctcgacgtga aagcaggcaa
     1981 tgtgaaaatt gtgctttccg gcgcagccaa aacgaaattg tccggcacgg ccacctcgct
     2041 cgacgcaacg ctgagcggcg catcgcagct gaaagcctac agcatgcagg cggaaaaagc
     2101 gaccattacg gcaagcggtg cttccgtagc gcatgtttcc gtcacttccg ccatcatggc
     2161 gacggcgagc ggtgctgctg aagtgcacta ccagggaaac ccggtggaaa agaacatcga
     2221 cgcttcgggc gcggcttccg tcggtgttaa gggcggcgac agcgatacgg ccagctttca
     2281 catcagcggg aaggacgttt acgtggtgga caacgacgac gatgacgaac gttcgccgcg
     2341 cgagaaaaaa gcggacgaca aggatttcga attctggtcg ggacttgatt tcggcgtgaa
     2401 cggttacctc actgccgaca acaagctcga tcttccgcag gccagcagct tcatgaacct
     2461 caattacgcg aaatcataca tgttcggctg gaacatctgg cagaaaaata tccacatcta
     2521 ccgcaacaac gtgaatctcg gtaccggcat cggtcttaac tggtaccact ataacttccg
     2581 cgaatcgtat tcgctgcagc ccgatcttcc gttcaccacc gctgttgcgg attcgctgaa
     2641 cttcacgaag aaccggctca acatgtgcta tgtcggcatc ccgctcttcc tcgagttcaa
     2701 taccaacaac agcgatgcgc gcaactcgtt tcacatcggc gcaggtgtca acgcaggcta
     2761 caacgttttt cgcaataagc tcaagcagcg ttacgactac gccggccgtg tgtacaagcg
     2821 taaagtcgtc aatgatttca acgtcgatcc gttccgtatc gacgccatgc tccgcatcgg
     2881 ttatggcaat tacaccatct tcggcgctta ctcgctgacc acgctcttcg aaaaaagcaa
     2941 gggccccacg gtttacccgt tcagcgcagg catccacgta gatttcggtc actgaacgcc
     3001 ggttatcatt taacatcccc cgcttatgag aaatcccgtg caatacctgc gcgggatttt
     3061 cgtttttgct aaattgtagg gaataaaaga aatcccatga aacggtcccg gtgtcttttt
     3121 ctctttttta tcgcgttcat tgccgcgctg ccggcgcagc agctggtgaa aaaaaagatc
     3181 gtctacgatt cgccgggcat cctgaaaaaa ggcatgaatg cgcacgaatt cttttttctc
     3241 ccgttcaacg taagcgatac gacttccgat gaaaccctgc tcagcgcgat ggattcggtc
     3301 ccgccgctga tcacgcaggt cgtttacgga acagatgcga aggacacgac gaaatacaat
     3361 gtcattatcg ccggctcgcg cctgtccgga acctattcgg ttacgcgcgt ttatcccgac
     3421 ggctgctttt ccggcacgca tacgtacggc aggaatcacc ggcttacggg aggttacgcc
     3481 acgtattatc ccggcaacca catcaaatcg gccggccagt acatggacgg aaagaagcag
     3541 gggcgatgga ggctctacga tgagaacggg cgcctcctgt ggaaagggaa atacaggaac
     3601 gacagcctcg tgaaagcgaa agattacggg cacagccgtt ttacgatcta tagtctttac
     3661 cgccaccgcg acctgcgtcc gtacaattac gtcatcgttt acgggaagga taccgcagcg
     3721 gattatttcc cggaggatac cgcgaacgtt tcccgccacc tgcctttcgg tatgaattgc
     3781 tccgcggaca tgtacacgat ggattttcac aacgcgcgca acagcatgcc gcaactgggc
     3841 ggctacccga acgattactg catcgccatg ggcgacgaga tttacgcagg ctaccgcgag
     3901 aaatttttcg tcaccgccgg gctccatttc aacctgccga aagaatttac cggcaattat
     3961 cccgggtacg gaaaagttgc ggtggaggcc gcgcagttcc gcgctcatct cgacgggaac
     4021 cttcccgtga tcaggaaccc gaagcgcacc ctgtttgttt acgggggcat catgttcgcg
     4081 cggacgtacc tcacgtcttt gcagcggtac gataacggca attcggtgag ctattttgaa
     4141 acgagccaca taaagcagag cctcgccgac gcgggtcttc ttttcgaatg cacggcccgc
     4201 ccgttcacac cgcgttacac cggttatttt tccattaagg cgggcgtcaa ttacccgatc
     4261 ggaaatgcgc agtggtacta ttttttcagc ccgcaggtca cgtaccccga aagcgggaaa
     4321 ccgggtgtga agctcggcgt gttttatttt tccgtaggtt tcacgatatg gaaatcagat
     4381 catcgccgga ccagcccgta acgtttttat gaataccatc cttcccgttc ccggcggtat
     4441 ttacctttcg ccaccgggtg aaaaagatat tcccgcattc gtgaaatgcc tcaacgaccg
     4501 ggaaattttc gatcgcacgc tgatgatccc gttcccgtac acggaaacgg atgcgctgaa
     4561 cttcctcgcc ttcgcgcacg atgcacgcag ccgttacggg catgagctga atttcgtcat
     4621 ccgtgatgcg aacggggaaa tgatgggcgg cgccggttac cacggtaaaa atttccattc
     4681 caccaatgcg caccgcgacg agctcggcta ctgggtggcg gaaccgtttc gcaataaacg
     4741 cgtgatgagc cgcgtgcttc ctgtcctcgt gcagtacggg atcgaggtgc gcaggctgaa
     4801 acgcatcgag gcgtgcatct atgcattcaa cgacgcgtcg gaacggctgc tgctgaagaa
     4861 cggtttcgtc atggaaggga ttttgcgcaa agcgtattac aagaacgcgc agttcttcga
     4921 tgcaaaatta ttttcactga tcgtatgaag gagcgcagct gcagcgcttt tcagtttcta
     4981 acttcctgcc ttccgctcgc ggagctcgct gcagtcaggg ccaggcaggt ttctttcgcc
     5041 cgtttcattt cctgttgttc gttccggcca tcgtccgtgt tacctgttcg tgttcttaca
     5101 ggcaggagag gaccgggaac ggcagtgctt tcgttcggat ttttactgcg gaacttcgga
     5161 tcttcctgtc ttcgtcgctt ttttcattta acggatcaaa attttatgcc ggtatcctgg
     5221 aaggttgttt tcttcttcgt tcttgttcaa acggcggcgc atgcgcaggg ctcggtctcc
     5281 ggaaagatcg tcgattccac gcaggccccg gttccgtttg tcgtcgtggc cctgcttcat
     5341 ccgggcgaca gtgcgatcag ggcaggaacg ctcacggatg aaagcggtca tttcgttttc
     5401 aggaatgtgc ggaccgggaa gtatttcctg aaaatttccg gcggtgcgta cgaagaacag
     5461 ttcagcgatg tgatcacatt cgacagcatt catgacctca ccgtgccgga cattgttctg
     5521 cgccggtcga aagtattcga tgcggttacg gtcacggcag tgaagcagac cgtcgtgttc
     5581 aggaacggga acacgattgt caatgtcggg aattccgcgc tggcgaaagg gaattcggtt
     5641 ttcgatctgc tgtcgaagct gccgggcgtg acggtcgaca acaacatcat ttccctacag
     5701 ggaaaaagcg gcgtcatcgt gatgatcgac aaccgcgtgc agcaggtttc gaacgatcag
     5761 ctcatcaacc tgctcaaaag catgagcgcg gaatcggtgg agcgtatcga gatactgaaa
     5821 aatccacccg tgaaatacga cgcttcggga acgtcgggca tgatcagcat catcaccaaa
     5881 aaaaatacgc agaaaggctt cagcgggaat gtgtacgcca ccacgtcgca gggattttac
     5941 aacaacagcg atgccggtct ttccctggat tacaggaacg acaggttctc tttcttttcc
     6001 ggcattgacg ctgacgacgg ctggtaccgg tcgcacgaaa agttttaccg gaatttcgtc
     6061 accggttccg gcgttacgca aatggtcaat gaaaatttca cgcgcgaacg cggatcggat
     6121 ctgaatttca ggatcggcgc ggactggtcg gtcagtcccc gtaccacgct gggcgcaaag
     6181 atcgacggag gtccgggcgc atacacatcc tatgaaaccg gcagcgacca ggttttcgat
     6241 tacaacagca ccgggttcga tcacctcgtt tccggtaatt acattccgaa cgtgtggaac
     6301 acggtcaatt ataacgtcaa cggcgaacgg cttttcgata cgctgggaac cgcgctgaat
     6361 ttttcttccg actttaccga tctccgcgaa acggatgaag gacacggctg gaattatttt
     6421 tacgatgcga acgggaacaa tgcgctgcct cccgacatca atcaaagcca taacaggtcc
     6481 gcaacgcaga tattttccgc ccggcttgat ttcgcgaaaa aaagagacac tgcgtcgtct
     6541 ttcgaagcgg gactgaaagc aagcgccgtt actaccggca acgattacct gttcgaacgg
     6601 caggataatt tcaccgggaa tttttacagc gatacggctt tcaccagcaa ttacatatac
     6661 agcgagcaga cgtatgccgg ttatgcgaat tattcccggg cgtggaaatc gttcagcatg
     6721 gagctgggaa tgcgggcgga agaaacggcg ctcagcgggc ataatcccca ttcagggttt
     6781 gcgatggcgg atcattactt caatgttttc ccggacatct cgcttgattt ttcaaaatca
     6841 gaagaccacg attttcagct gaacctgaac cgcaggatcg accgccccga tttcgacgag
     6901 ctgaatccgt tcatctatta ccgcgatccg ttttcctatt tccggggcaa ccagtttctc
     6961 cgcccgcaat acagcgaccg tgcggagctc acctacagct accagggcgc tatcgtcaat
     7021 tcggtttcgt acgagcggct ggacaaattc gtcctcgact acacggtaca gaacgacagc
     7081 acgaagatct tcctggaaac ggaaaagaac gtgagttccg ccaccgagtg cacgtacagc
     7141 cttttcgtgc aggaagatgt tttcccttgg tggtcggtaa cggcgacagg gaatttcctg
     7201 ttgctcgatt tcaggggaga tgtgcagggc gttcctttcc atacgcgcgg cagcagctat
     7261 tatgcgaatg tgatgaacac cctcattttt ccgcataaga caaacctgga agtgatgttc
     7321 atgtaccgcg ga
//
LOCUS       DASTSF010000315         4713 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_509037,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000315 DASTSF010000000
VERSION     DASTSF010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4713
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_509037"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1188
                     /gene="pyk"
                     /locus_tag="VFU15_09045"
     CDS             <1..1188
                     /gene="pyk"
                     /locus_tag="VFU15_09045"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEU4717969.1"
                     /translation="GVELKDDAEIIFTTEKMIGRADRVYITYPQFPKDVKPGENILVD
                     DGKLVLNVVSTNGKDEVVAKVLHGGILSSKKGVNLPNTKISLPCLTEKDMRDLVFALE
                     NNFDWIGLSFVRSGADIIELKHLIKNINPESHVRVIAKIEKPEAILDIDNIVHETDAL
                     MVARGDLGVEVPMQRVPVLQKMLVRKCREASKPVIIATQMMESMITNISPTRAEVNDV
                     ANSVMDGADAVMLSGETSVGKHPAKVVQAMSKIIEQVEEEHDIYYSDRVPNFHSDERY
                     ISDNICYAATQLAKQVDASAIITMTFSGYTAFRVSSYRPKANIFVFTGNYSILTQLSL
                     VWGVRGFYYDKSISTDHTIADIMFSLKKDEMVMKNDFIINIASIPLNEKGRTNMIKLS
                     VVD"
     gene            complement(1242..1550)
                     /locus_tag="VFU15_09050"
     CDS             complement(1242..1550)
                     /locus_tag="VFU15_09050"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU4717970.1"
                     /translation="MNRLEQLLQMQREDPQDPFLAYAIALEHAKEGKRAQAISEVEKL
                     LEEKPGYLGAYYQLGQWYEQEDKNEEAIRVYEKGISVAQQQHNHKALGELRTALDMIT
                     "
     gene            complement(1593..2606)
                     /locus_tag="VFU15_09055"
     CDS             complement(1593..2606)
                     /locus_tag="VFU15_09055"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016194607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEU4717971.1"
                     /translation="MKERIESLMKEVETFSASSSEQAEQFRLKYLSRKGEISVLFEEF
                     KTVPAEMKKEVGQLLNRLKISAQEKLDAFKNSGGAKNETKEKETDLTLPSVYFRGGRH
                     PLNIVRKEILGIFGRIGFTVADGPEIEDDWHNFSALNFPEEHPARDMQDTFFIGDDIA
                     LRTHTSSVQVRVLENTQPPVRIVMPGRVYRNEAISARAHCFFHQVEGLYVDKNVSFAD
                     MKQALLYFSREMFGEKTEIRLRPSYFPFTEPSAEMDISCTICHGKGCAVCKHSGWVEI
                     LGCGMVDPAVLKNCNIDPDVYSGFAFGMGIERITMLKYGVNDLRLFSENDVRFLSQFG
                     SEI"
     gene            complement(2632..3288)
                     /locus_tag="VFU15_09060"
     CDS             complement(2632..3288)
                     /locus_tag="VFU15_09060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717972.1"
                     /translation="MKFFFPLFFSVFYFASCGPHHPGSSPGFDETDFFRGKLRADVKT
                     SVATGGTDSFTTIKALHHWLPGDEYMHTETDANKQDAPRTAEENRNVVVTDLFIYGVK
                     REDDNDFHLILASYGKTGKDQPYMTAEISGLPDSSDPAFPALAAVRKKFEDHFGDDTK
                     KEIVFVADRNHPPVHLVSISGSLFFDNHHYGEHSSVQKYKVCTAWEIHPVTGIRFDDQ
                     "
     gene            complement(3285..4070)
                     /locus_tag="VFU15_09065"
     CDS             complement(3285..4070)
                     /locus_tag="VFU15_09065"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEU4717973.1"
                     /translation="MVKRILLPALMVVMIALLTSYKSADTRSKVLIHTSFGDIKIVLY
                     DETPKHRDNFLKLVQNHTLDSTLFHRVIPQFMIQGGDVDSKHAKPGQFLGNGTLGYTI
                     PAEFNPNKESSACQFYIVQGKTYTDSMLNLTQAGLDQQVKQALFTKMINAPENAGLKS
                     AFIRAQMRYQQTRNADSLNYYSGIINPQIDAEFAKTPHRVITDDQRKVYETIGGTPQL
                     DGGYTVFGEVVSGLNVVDSIANTKRDQYDRPLTDIRMTMKIVK"
     gene            4168..4521
                     /locus_tag="VFU15_09070"
     CDS             4168..4521
                     /locus_tag="VFU15_09070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717974.1"
                     /translation="MNKLLKRLSLAGVAVVMVFGMTTISGCGKNKPCKAVITVVSAST
                     GAPIAGAQLLLQPPSTATSGTPIQPMTGSTDASGVANFETPLPKILDIIVNNWASGKV
                     VRFEEGKTDNVTVQY"
BASE COUNT         1056 a         1289 c         1259 g         1109 t
ORIGIN      
        1 ggcgtggagc tgaaggacga tgcggaaatt attttcacca ctgaaaaaat gatcgggcgc
       61 gcggaccggg tttacatcac ctacccgcag ttcccgaaag acgtgaagcc cggcgagaac
      121 attctcgtcg acgacggcaa gctggtgctg aacgtcgttt ccacgaacgg gaaggacgag
      181 gtcgtggcga aagtgctgca cggcgggatc ctttcttcga aaaaaggcgt caatcttccc
      241 aacacgaaga tctcgctgcc ctgccttacc gaaaaagaca tgcgcgatct cgtgttcgcg
      301 ctggaaaata atttcgactg gatcggcctt tccttcgttc gttctggcgc cgacatcatc
      361 gagctgaaac acctcatcaa gaacatcaac ccggaaagcc acgtgcgcgt catcgcgaag
      421 atcgaaaagc cggaagcgat actcgacatc gacaacatcg tgcacgaaac cgatgcgctc
      481 atggtggccc gcggcgatct cggcgtggaa gttcccatgc agcgcgttcc cgttctgcag
      541 aaaatgctcg tgcgcaaatg ccgcgaagct tcgaagcccg tcatcatcgc cacgcagatg
      601 atggaaagca tgatcacgaa catttctccc acgcgcgccg aagtgaacga cgtcgcgaac
      661 tccgtcatgg acggcgccga tgcggtgatg ctcagcgggg aaacgtcagt gggaaaacat
      721 cccgcgaaag tcgtgcaggc gatgtcgaag atcatcgaac aggtggaaga agaacacgac
      781 atttattact ccgaccgcgt gccgaatttc cacagcgacg aacgttacat ttccgacaac
      841 atctgctatg ccgcgacgca gctcgccaag caggtcgacg ccagcgccat catcacgatg
      901 acgttctcgg gctacactgc gttccgcgtt tccagctacc gcccgaaggc gaacattttt
      961 gtcttcacgg gaaactattc catcctcacg cagctcagcc tcgtgtgggg cgtgcgcggt
     1021 ttctattacg acaaaagcat cagcaccgat cacaccatcg ccgacatcat gttctcgctc
     1081 aagaaagacg agatggtgat gaagaacgat ttcatcatca acatcgccag cattcccctg
     1141 aatgaaaaag gcagaacgaa catgatcaaa ctgagcgtgg tggattgatt cacgttccgg
     1201 ttttccgtct gtaaaaaaac cagacgcggg ccccggaatg tttatgtgat catgtccagc
     1261 gccgtgcgca attcgcccag cgctttgtga ttgtgctgct gctgcgcaac agaaattcct
     1321 ttttcgtaaa cacgtatcgc ctcttcgttt ttgtcttcct gctcgtacca ttgcccgagc
     1381 tggtaatacg caccgaggta acccggtttt tcttccagca gcttttcgac ttccgagatt
     1441 gcctgcgcac gttttccttc cttcgcatgc tcgagcgcga tcgcgtacgc gaggaacgga
     1501 tcctgcggat cttcccgctg catctgtaaa agctgttcga gacggttcat gtaaaatgcg
     1561 ctgcgctttt tcgcacccgc gcgtctttgc ggttatatct ccgaaccaaa ctgcgaaagg
     1621 aaacgcacat cgttctcgga aaaaagacga aggtcgttca cgccgtattt cagcatcgtg
     1681 atgcgttcaa tgcccatgcc gaaagcgaaa ccgctgtata catccggatc gatgttgcag
     1741 tttttcagca ccgccgggtc aaccattccg cagccgagga tttccaccca gccggaatgc
     1801 ttgcacactg cgcagccttt gccgtggcag atcgtgcagg aaatatccat ctctgcgctc
     1861 ggctcggtga acgggaaata ggaagggcgc aaacgtattt ccgttttctc accgaacatc
     1921 tcgcgcgaga aatacagcag cgcctgtttc atatcggcga acgaaacgtt tttgtccacg
     1981 taaagtcctt ccacctggtg gaagaaacag tgcgcacgcg ccgagatcgc ttcgttgcgg
     2041 tacacacgcc cgggcatgac gatgcgcaca ggcggctgcg tgttttcgag cacgcgcacc
     2101 tgcacgctcg aggtgtgcgt ccgcagcgca atgtcgtcgc cgatgaaaaa tgtatcctgc
     2161 atatcgcggg cgggatgctc ttcggggaaa ttcagcgccg agaaattgtg ccagtcgtct
     2221 tcgatctcgg gaccgtcagc aacggtaaaa ccgatgcgac cgaagatgcc gagtatttct
     2281 ttccgcacga tgttcagcgg gtgacgtccg ccacggaaat acacggaagg aagcgtaagg
     2341 tccgtttcct tttctttcgt ttcgtttttc gcccctcctg aatttttgaa cgcgtcgagc
     2401 ttttcctgcg ccgaaatttt cagccggttc aggagctgcc cgacctcttt tttcatttcg
     2461 gcgggaaccg ttttgaattc ttcgaagagc acggagatct cgcccttgcg gctcaggtat
     2521 ttcagcctga actgctcggc ctgttcggaa gaagaggcgg aaaatgtttc cacctccttc
     2581 atcagcgact cgattctttc tttcatcggg agacaaagtt agatgaattc gtcactggtc
     2641 gtcaaagcgg ataccagtca cgggatgaat ttcccatgcc gtgcacactt tgtatttctg
     2701 tacggaagag tgttcgccat aatgatgatt gtcgaaaaaa agcgaaccgc tgatggagac
     2761 gagatgcacg ggcgggtggt tcctgtcggc gacgaacacg atctcctttt ttgtgtcatc
     2821 gccgaaatga tcctcgaact ttttccgcac ggccgccagc gccggaaatg caggatcgga
     2881 agaatcgggc agcccggaaa tttccgccgt catgtacggc tggtctttgc cggtttttcc
     2941 gtacgaagca agaatgaggt ggaaatcgtt atcgtcctcg cgcttgacgc cgtaaatgaa
     3001 aagatccgtc acgacgacat tccggttctc ttccgctgtt cgcggagcgt cctgcttgtt
     3061 cgcatcggtt tccgtgtgca tgtattcatc gccgggaagc cagtgatgca gcgccttgat
     3121 cgtggtgaat gaatcggtcc cgcccgtcgc gaccgacgtc tttacatccg cgcggagctt
     3181 tccccggaaa aaatccgttt cgtcaaagcc cggcgaagag cccggatggt gcgggccgca
     3241 ggaagcaaaa taaaaaacag aaaagaacag cgggaagaaa aacttcattt cacgatcttc
     3301 atcgtcatgc ggatgtccgt cagcggacga tcatactggt cacgtttggt gttggcaatg
     3361 ctgtcgacca cgttgaggcc ggaaaccact tcgccgaaaa ccgtatagcc cccgtcgagc
     3421 tgcggcgttc ccccaatggt ctcatacact ttgcgctgat catcggtgat cacgcggtgc
     3481 ggtgttttcg caaactccgc atcgatctgc gggttgatga tgccggaata ataattcagg
     3541 ctgtccgcgt tgcgcgtctg ctggtaacgc atctgcgcgc ggatgaaggc gcttttcagc
     3601 ccggcgtttt ccggcgcgtt gatcattttc gtgaacagcg cctgcttcac ctgctggtcg
     3661 aggccggcct gcgtgaggtt gagcatgctg tcggtgtacg tttttccctg cacgatgtag
     3721 aactggcagg cggacgattc cttgttcggg ttgaattccg caggtatggt gtaacccagc
     3781 gttccgttcc cgaggaattg cccgggcttc gcatgcttcg aatcgacatc gccgccctgg
     3841 atcataaact ggggaatgac gcggtgaaaa agcgtgctgt cgagcgtatg attctgcacg
     3901 agcttgagga agttgtcgcg gtgcttcggc gtctcgtcgt agaggacgat tttgatgtct
     3961 ccgaaacttg tgtggatcaa taccttagaa cgcgtgtctg ccgacttgta agacgtcagc
     4021 aaagcgatca ttacgaccat caatgcagga agcagaatcc tctttaccat gttgaaaact
     4081 tttttatatt tgtaagttgt aaaggtagct attcggctac tttgcacaaa aaccaggccc
     4141 tgttcctgtt ttttaaccag gcaaacgatg aacaagcttc tgaaacgtct ctcattggca
     4201 ggtgttgctg tagtaatggt cttcggcatg acgaccattt ccggttgtgg aaagaacaaa
     4261 ccctgcaagg ctgtgatcac ggttgtgagc gcgtccacgg gggcgccgat cgcaggagcc
     4321 cagctgttgc ttcagccgcc ttccacagcc acttccggaa cgcccatcca gccgatgaca
     4381 ggatcgacgg atgcatccgg cgtagccaat tttgaaacgc cgcttcccaa gatcctcgac
     4441 atcatcgtca acaactgggc ttcgggaaaa gtggtgcgtt tcgaggaagg aaaaacagac
     4501 aacgttaccg tacagtactg aaaaatattt tttgcgataa accgaagccg ccggattccc
     4561 ggcggttttt ttattgcatt tccacctgtt tccggaaata cacgatgatc gcctcgcgga
     4621 tgagccggtg ctgctgggca ggtttcaggt gcggcagctt tgccgtcgct ttccagtgcg
     4681 gccatttgtc ggcgtcgcgg ccttcgaatt cgt
//
LOCUS       DASTSF010000316         5085 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1796550,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000316 DASTSF010000000
VERSION     DASTSF010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5085
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1796550"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            82..1482
                     /gene="glmM"
                     /locus_tag="VFU15_09075"
     CDS             82..1482
                     /gene="glmM"
                     /locus_tag="VFU15_09075"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008625072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEU4717975.1"
                     /translation="MALIKSISGIRGTMGGKPGEGLTPTDIIRFTSAFGSWIMNRTKS
                     KNVRIVVGRDARISGDMVNRLVTGTLIGLGIDVTDVGLSTTPTVEMAVPAEKAAGGII
                     LTASHNPKQWNALKLLNEKGEFISAADGEEVLRIAESDNVVFADVNKLGKITENSSYI
                     KYHIEKVLALPYVDVKAIRERKFRIVVDAVNSTGGIAVPELLRALGVDDIVELYCEPN
                     GDFPHNPEPLPENLRDISKAVVKNKAHLGIVVDPDVDRLALVCEDGEMFGEEYTLVSV
                     ADYVLKHKKGNTVSNLSSTRALRDVTEKAGGKYFASAVGEVNVVEMMKANDAVIGGEG
                     NGGVILPALHYGRDALAGIALFLSHLAKFGKSASMLRAAYPDYHISKNKIELTPEINV
                     DDILDGIRVKYKKQPVNTVDGVKIEFDKEWVHLRRSNTEPIIRIYSESESETTAENLA
                     KKIITDIRELIKLQQV"
     gene            1602..2741
                     /locus_tag="VFU15_09080"
     CDS             1602..2741
                     /locus_tag="VFU15_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014680597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HEU4717976.1"
                     /translation="MKAYFDNAATTQLDKEVLEAMLPLMTEHYGNPSSIHAWGRQTRA
                     AIEQARKTVARLLNCSPSEIFFTSGGTEADNMAIRSSIEGLGLKHAITSPIEHHAVLH
                     TLEALADKGQVKLSLVKLTDNGHIDLNDLEQLLKNNERSFVSLMHANNEIGDLLPIKK
                     VGEMCAKYNAVFHSDTVQTVAHYSIDLKEIPVHIINCAAHKFHGPKGVGFIYINGDIH
                     VPPLIFGGAQERNMRGGTENVYGIIGLAKAMEIAYRDLKKHQEHIQGLKTYMIGQLEK
                     NLPGVLFNGDAKGSSLYTVLNVCLPPTENAEMLVFNLDINGIAASAGSACSSGSNVGS
                     HVLRAIGTDMSRPSVRFSFSKYNTKEEIDYTVGKLKELFPVAVKG"
     gene            2796..2867
                     /locus_tag="VFU15_09085"
     tRNA            2796..2867
                     /locus_tag="VFU15_09085"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2828..2830,aa:Thr,seq:cgt)
     gene            3007..3690
                     /locus_tag="VFU15_09090"
     CDS             3007..3690
                     /locus_tag="VFU15_09090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717977.1"
                     /translation="MFFLIAALFILHGCGDTSASGDKQLAGKIDSLSRKLDSVTAMLA
                     RKDSLKETVAKKDTVLAISPLPKTAKQDTGNRTTSPAKKTAVVQKSPGAGDTTFYYYA
                     SSPKRISVIITPWDPHQKRKVMFFDRSGTKTYEIEDERMSYSSTTEINSFYPDGGASK
                     ATTHMNPGASMYWYETYITFGPDNEPQWKESQKFPQQNLSLPDKYYWDAGSKSWKKQE
                     IVHEQDVPH"
     gene            complement(3703..4350)
                     /locus_tag="VFU15_09095"
     CDS             complement(3703..4350)
                     /locus_tag="VFU15_09095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717978.1"
                     /translation="MNWLPRIFFFFLCSVSVPALNAAAGSDTLNRYNIHHKKNGFWVQ
                     YLDSVLNPVDSVNAFYYGYEKYDNGDQVFRFFAPWPNKLQGYRFSHEGQSPVKGVPVL
                     LDGTFTWYYASGVNSNLVAEIQVFRNGRPVYFNTYHRTASDTVSFFNEILYFDRRYNG
                     VDGTFYYEETSNEPGVTPGPIVTYSGYFRKGKHKWSMHGKKTPGLKKKYGISIIS"
     gene            complement(4474..5064)
                     /locus_tag="VFU15_09100"
     CDS             complement(4474..5064)
                     /locus_tag="VFU15_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012925138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEU4717979.1"
                     /translation="MNEENDSYVIYKPYGVLSQFTPEGNRPALGSLFRFPKDCYPVGR
                     LDAESEGLLIISNDASLNRKLLDPVNEHARTYFVQVDGEISEEAVAQLRSGVEIKVEG
                     KSYRTKKSVAEKIAEPFLPASVPPVRFRKSIPTSWISLTLAEGKNRQVRHMTAAVGFP
                     TLRLVRVKIENLELGNMQPGEVRKIKGSELKRLLKL"
BASE COUNT         1277 a         1304 c         1312 g         1192 t
ORIGIN      
        1 aaatcctgtg ttagtatatt ttcccgccga tgaaaaacaa ctgcaaaacg tggaactatc
       61 tttgcaatcc caatttaata catggctctg ataaaatcta tttccggcat tcgcggaaca
      121 atgggcggca aaccgggcga aggattgacc cccacggata tcatacgatt tacttctgca
      181 ttcggctcct ggataatgaa caggacgaag tccaaaaacg tacgcattgt ggtcggacgg
      241 gacgcccgca tttccggcga catggtgaac cggctcgtga caggaacgct catcggtctt
      301 ggcattgatg ttaccgatgt gggactttcc accacgccta cggttgaaat ggcggttccc
      361 gccgaaaaag cggcaggcgg catcattctt acagcgagtc ataacccgaa gcaatggaat
      421 gcgctcaagc tgctcaacga aaaaggagaa ttcatttccg cggctgacgg ggaagaggtg
      481 ctgcgcattg cggaatccga taatgttgtt ttcgccgatg tgaacaagct tgggaaaata
      541 acagagaaca gcagctacat aaaataccac atcgaaaaag tgctggcgct tccatatgtc
      601 gacgtgaaag cgatacgcga aaggaaattc aggatcgtgg tcgatgctgt caactcgacg
      661 ggcgggatcg ctgtgccgga attgctccgt gctttgggtg tggacgatat cgtggagctg
      721 tattgcgagc ccaacgggga tttcccgcac aatcccgaac cattgccgga aaacctccgt
      781 gatatttcca aagccgttgt caagaacaaa gcgcatctcg gcattgtcgt cgatccggat
      841 gtggaccgcc tcgcgctcgt gtgcgaggac ggggagatgt tcggcgagga atacacgctg
      901 gtttctgttg ctgattatgt gctgaagcat aaaaaaggca atacggtttc gaatctttct
      961 tccacgcgcg cgctgcgcga tgtgacggaa aaagcgggtg gaaaatattt tgcttccgca
     1021 gtgggagagg tgaacgtcgt ggaaatgatg aaggcgaatg acgcggtgat cgggggcgaa
     1081 ggcaacggcg gtgttatcct gcctgcgctt cattacggcc gcgatgcgct ggcgggtatt
     1141 gcgctcttcc tttcacacct cgcgaaattc ggcaagagcg catccatgct ccgggccgct
     1201 tatcccgatt atcacatttc caagaacaag atcgaactca cgccggagat caacgtggac
     1261 gatatcctcg acggcatccg cgtgaaatac aagaagcagc ccgtgaatac ggtcgacggc
     1321 gtgaagatcg agttcgacaa agaatgggtg catctccgcc gctcgaacac ggagccgatc
     1381 atccgcatct attcggaaag cgaatcggaa accaccgcgg aaaatcttgc aaagaagatc
     1441 attaccgata tccgcgagct gatcaagctg cagcaggtgt aacagggttt gcctttctta
     1501 gccaatcctg attcagaatt gactttttat gttctcaaaa ccgggagata ttccgatctt
     1561 ccatcggtga atattacctt tgcaccacca aaaccaatcc gatgaaagcc tatttcgata
     1621 acgccgcaac gacgcagctc gacaaagaag tgctcgaggc catgcttccg ctgatgaccg
     1681 agcattacgg caacccgtcg tccattcatg cctggggccg gcagacacgt gccgcgatcg
     1741 aacaggcgag gaaaacggtg gcgcgcctgc tgaactgttc gccttccgaa attttcttta
     1801 cgtcgggtgg aacggaagcc gacaacatgg ccatccgcag cagcatcgaa ggacttggac
     1861 tgaagcacgc gatcacgtcc ccgatcgagc accacgctgt gctgcatacg ctcgaggcgc
     1921 tggcagacaa aggccaggtc aagctcagcc tcgtgaagct caccgacaac gggcacatcg
     1981 atctcaatga tctcgaacaa ctgctgaaaa acaacgagcg ttctttcgtt tcactcatgc
     2041 atgcgaacaa cgagatcggc gacctgctcc cgatcaagaa agtgggcgag atgtgcgcga
     2101 aatacaacgc ggtttttcat tcggatacgg tgcagaccgt tgcgcattac agcatagacc
     2161 tcaaggaaat tcccgttcac atcatcaact gcgcagcgca taagttccac ggaccgaaag
     2221 gcgtcgggtt catttacatt aacggagata ttcacgttcc gccgctgatc ttcggcggtg
     2281 cacaggaacg caacatgcgc gggggaacgg aaaatgtgta cggcattatc ggcctggcga
     2341 aagcgatgga gatcgcttac cgcgacctga agaagcacca ggagcacatc cagggattga
     2401 aaacttacat gatcggccag ctcgagaaga acctcccggg cgtgctgttc aacggcgatg
     2461 ccaaaggaag ctcgctctac acggtgctca acgtttgtct tcctcccacg gaaaacgcgg
     2521 aaatgctggt cttcaacctc gacatcaacg ggattgcggc ttccgcggga agcgcctgct
     2581 cttcgggcag caatgtaggc tcgcacgtgc tgcgcgccat cggcacggat atgtcgcgtc
     2641 cttccgtccg tttttcattc agcaaataca acacgaagga agagatcgat tacacagtcg
     2701 gcaagctgaa ggagctgttc cccgtggcgg tgaaaggata aattttccta ttgtttttga
     2761 aatagcgcgg aaatcttaat tttgcgcctc cattcgccac cttagctcag gggtagagca
     2821 gctgattcgt aatcagcagg tcgtgggttc aaatcccatg ggtggctctc caaagttctt
     2881 cgcctgaaga acttttttta tgcggttacg ctacaagcct gctttctgat cgccggaaaa
     2941 aattattttg ctgaaaatgg cccgcgcttt gcgtggcatc acattacgat gaaaaggata
     3001 tcccgcttgt tttttttaat cgcagcttta tttatcctgc atggatgtgg tgatacatcc
     3061 gcttccggcg acaagcagct ggccgggaag atcgattcgc tttcccggaa gctggacagc
     3121 gtcaccgcta tgcttgcgcg gaaggattcg ctgaaggaaa ctgttgcgaa gaaagatact
     3181 gttttagcaa tttcaccttt gcccaaaacg gcaaagcagg acacgggaaa caggaccaca
     3241 tcgccggcga agaaaacagc ggtcgtacag aaatctcccg gcgcgggcga tacgacgttt
     3301 tactattatg cttcttctcc gaaaaggatt tccgtcatca ttacgccctg ggacccgcat
     3361 cagaaacgta aagtgatgtt cttcgaccgt tccggaacca aaacgtacga gatcgaagac
     3421 gagcgcatga gctattcatc cactaccgaa atcaacagct tttacccgga cggcggcgct
     3481 tcgaaagcaa ccacgcacat gaatccgggc gcgagcatgt attggtatga aacctacatc
     3541 acctttggtc cggacaatga accgcaatgg aaggaatcgc agaaattccc gcagcagaac
     3601 ctttcgcttc ccgataaata ttactgggat gccggctcga agtcgtggaa gaagcaggag
     3661 atcgtgcatg agcaggacgt tccgcattga taaaccggaa aatcagctga tgatggaaat
     3721 gccgtacttt tttttcagtc ccggcgtttt tttaccgtgc atgctccatt tgtgttttcc
     3781 tttccggaaa taacctgaat aggttacgat cggtccgggc gttaccccgg gttcattgct
     3841 tgtttcttca taatagaagg tgccgtcaac gccattgtac ctgcgatcga aatagagaat
     3901 ttcattgaag aaagaaacgg tgtcactcgc cgtgcggtga tacgtgttga aataaacagg
     3961 ccgtccgtta cggaacacct ggatctccgc aacaaggttg gaattcacgc ctgaagcgta
     4021 ataccacgtg aatgttccgt cgagcaaaac cggaactcct ttcaccggtg actgtccttc
     4081 atgcgagaac cggtagccct gtaatttgtt cggccacggc gcaaaaaaac ggaacacctg
     4141 gtcaccgttg tcgtatttct cgtacccgta ataaaatgcg ttcacgctgt ccaccgggtt
     4201 cagcacgctg tcgaggtatt gcacccagaa accgtttttc ttatgatgta tgttgtaccg
     4261 gttgagcgta tcgcttcctg ccgccgcatt caacgcaggt acggaaacag aacaaaggaa
     4321 aaagaagaaa atccttggaa gccagttcat gcacatgaaa tttaatgttc tgatcggggt
     4381 attccttcgc tgtgctgtgc tttgcaaaag tattttcttc gtgcgcattg ctggccaggt
     4441 tgttgtcgga tgataatcat taccggatgc ggatcacagc ttcagcagtc ttttcaattc
     4501 gcttcctttt attttccgca cttcacccgg ctgcatattt ccgagctcga ggttttcgat
     4561 tttcacgcgc acgagacgta atgtagggaa acccacagcc gccgtcatgt ggcgcacctg
     4621 ccggtttttc ccttccgcca gtgtcagcga gatccaggag gtaggaatgg atttgcgaaa
     4681 acgcacaggc ggaacgctgg caggtaaaaa aggttctgca attttttccg cgactgattt
     4741 tttcgtgcgg tacgattttc cttccacttt tatttcaaca cccgaacgca attgcgcaac
     4801 cgcttcctcg ctgatttccc cgtctacctg aacgaaatac gttcttgcgt gttcgttcac
     4861 gggatcgagc agtttcctgt tcagcgaagc gtcgttggaa atgatgagca gaccttcgct
     4921 ttccgcatcg agacggccga cggggtagca gtctttcgga aagcggaaaa gacttccgag
     4981 cgccggcctg ttcccttcag gggtgaattg tgacaatacg ccatagggtt tgtaaataac
     5041 ataactgtcg ttttcctcgt tcatgctgtc aaattaacat atcct
//
LOCUS       DASTSF010000317         2442 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_734206,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000317 DASTSF010000000
VERSION     DASTSF010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2442
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_734206"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..260
                     /locus_tag="VFU15_09105"
     CDS             <1..260
                     /locus_tag="VFU15_09105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717980.1"
                     /translation="WALPEAPFCAAAGEKNELLILSERHLFEFDPSDDSTHTLLKNGF
                     WDLLYPNSMVLLDHQLYIGMRSGVLRVDYEHPEKQDWFTNE"
     gene            461..>2442
                     /locus_tag="VFU15_09110"
     CDS             461..>2442
                     /locus_tag="VFU15_09110"
                     /inference="COORDINATES: protein motif:HMM:NF039587.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HEU4717981.1"
                     /translation="MKLLRPAILLCFACSGVMAGNQQDTSIVRKKFSCVRITQPVKID
                     GKLDDAVWQDAPIATNFIQNSPKEGAAASYQTEVKMLYDNNALYIGAMMYDDHPDSIM
                     KQMGLRDDNVTADNFRMVFDTYNTQQDAFDFTVTASNVQVDSRFSDFNYNAVWHSHVQ
                     MLPNGWSAEVEIPWSALRFPTTEKQNWGIQFTRWIQRKFEFDQWSLTPKAAQNSLKYW
                     GILEGLENIDVPVRLSVTPYLSTIWQSDNRYGPDSPSTSFGGGMDLKYGINESFTLDM
                     TLLPDFSQVQSDNIVKNLSPFEIQYDEYRPFFTEGTDLFSLGDLFYSRRIGKTPTQFY
                     NTGDLLDSNERIIKNPTQSRLLNATKLSGRTSNGMGIGILNAYVDNTYAVAQDTLTGA
                     TRKILTEPRSNYNIFVFDKQVQNGSHVYFVNTNVMRTHGYRSADVSGAGFSLNNKKST
                     WTFSGFGAVSNIMEPVDSLKGQFNSSLGYKYVASIDKNSGKLKYGISRRELGKNFDPN
                     DLGINYETNIADNSAYVAYNVYNPWWIFNLINSSLNFDYTQNARLHTMNNFTIDFFTY
                     AEFRNFWSFYIGSESTPFDSKDYYEPREDGRFYLRTGYNAVYGGINTNSNKKISFNLG
                     FHGGQTHYISQEIPVNPWGGADLTVNVRAGYRFSFSA"
BASE COUNT          645 a          671 c          583 g          543 t
ORIGIN      
        1 cctgggcgtt acccgaagcg cctttttgtg ctgcggccgg agaaaagaat gagctgctga
       61 tcctctcgga acgtcatctt tttgaattcg atccgtcgga cgactcaaca catactttgt
      121 taaaaaacgg gttctgggac ctactctatc ccaattcgat ggtgttgctg gatcatcaat
      181 tatacattgg tatgcgtagc ggggtattgc gggtcgatta tgaacacccg gaaaagcagg
      241 attggtttac gaatgaataa cggtacaatt aagatcgtac tcggaacatc aaatcccgga
      301 cacaatttga aagctggaaa cccggctccc aaccggaagc tgaaaacttt ctgaaaccga
      361 aatccattcc tgcctttcac aatttttagc tccctttcat cacctttctc tttcccggcc
      421 gttttattta tcccacctta ggcgcgacaa acgctccttc atgaaacttc tcagaccggc
      481 gatattgctg tgtttcgcat gcagcggagt catggccggg aaccagcagg acacctccat
      541 tgtgcggaag aaattctcct gcgtgaggat tacgcaacct gtgaagatcg atggaaaact
      601 cgacgacgcc gtgtggcagg atgcaccgat cgcgacgaat tttattcaga acagcccgaa
      661 agaaggcgcg gcggccagct accagacaga agtaaagatg ctgtacgaca acaacgcgct
      721 ttacatcggt gcgatgatgt acgacgatca tcccgacagc atcatgaagc agatgggact
      781 gcgcgacgat aatgtaaccg cggataattt ccgcatggtg ttcgatacgt acaacacgca
      841 gcaggatgcg ttcgatttta cggtgacggc ttccaacgtg caggtcgatt cgcgcttttc
      901 ggatttcaat tacaacgccg tctggcattc ccacgtgcag atgcttccca acggctggag
      961 cgcggaagtg gaaattccct ggtcggcgct gcgttttccg acaacggaaa agcagaactg
     1021 gggaattcag ttcacccggt ggatccagcg caaatttgaa ttcgaccagt ggtcgctgac
     1081 accgaaagca gcgcagaatt cgctcaaata ctggggcatt ctcgaagggc ttgaaaacat
     1141 cgacgtcccc gtgcggctga gcgtcacacc ttatctttcc accatctggc aaagcgacaa
     1201 ccgctacggc cccgattcac cgtccacgag cttcggcggc ggaatggacc tcaaatacgg
     1261 catcaacgaa agcttcacgc tcgacatgac gctcctcccc gatttcagcc aggtgcagag
     1321 cgacaacatc gtgaagaacc tgagcccgtt cgagatccag tacgacgaat accggccgtt
     1381 ctttaccgag ggaaccgatc ttttctcgct cggtgatctt ttttattcaa gacgcatcgg
     1441 gaaaacacca acgcaattct acaacaccgg cgacctgctc gactccaacg aacgcatcat
     1501 taaaaatccc acacaatcac gattgctgaa tgccacgaag ctttccggga gaaccagcaa
     1561 cggaatggga attgggatac tcaatgcgta cgtcgacaac acgtatgcgg tggcgcagga
     1621 tacgctaacc ggcgccacgc ggaaaatact caccgagcca cggtcgaact acaacatttt
     1681 cgttttcgac aagcaggtgc agaacggctc gcacgtgtat ttcgtgaaca cgaacgtcat
     1741 gcgcacacac ggttaccgca gcgcagatgt ttccggcgca ggattcagcc tcaacaacaa
     1801 aaaaagcacg tggaccttca gcggtttcgg tgccgtgagc aacatcatgg aaccggtcga
     1861 ttcgctgaaa ggtcaattca attcatcgct gggctataaa tacgtcgcat ccatcgacaa
     1921 gaacagcggg aaactgaaat acggcatcag ccgaagggaa ctcggcaaaa atttcgatcc
     1981 gaacgatctc ggcatcaact acgaaacgaa catcgccgac aactccgctt acgttgccta
     2041 caatgtttac aatccctggt ggatcttcaa cttaatcaac tcgagcctga atttcgatta
     2101 cacccagaat gcgcgcctgc atacgatgaa taatttcacg atcgattttt tcacgtacgc
     2161 cgaattccgc aacttctggt cgttctacat cggcagtgaa tccaccccgt tcgactcgaa
     2221 agattactac gagccgcgcg aggacgggcg cttctatctc cgcacggggt acaatgctgt
     2281 ttatggagga atcaacacaa actccaataa aaaaatatca ttcaacctcg gctttcacgg
     2341 cggacaaacg cattatatct cgcaggaaat tccggtcaat ccctggggcg gcgctgatct
     2401 tacggtcaat gtgcgcgccg ggtaccgttt ttccttctct gc
//
LOCUS       DASTSF010000318         8004 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_394381,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000318 DASTSF010000000
VERSION     DASTSF010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 8004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8004
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_394381"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..299)
                     /locus_tag="VFU15_09115"
     CDS             complement(<1..299)
                     /locus_tag="VFU15_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017259572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEU4717982.1"
                     /translation="MKIAVVGATGLVGTKMLEVLAERNFPVTELIPVASERSAGKEIS
                     FGGKKWKVVTPEKAIAMKPQIALFSAGGSTSLEWAPKFAEAGTIVIDNSSAWRMDP"
     gene            780..4208
                     /locus_tag="VFU15_09120"
     CDS             780..4208
                     /locus_tag="VFU15_09120"
                     /inference="COORDINATES: protein motif:HMM:NF013125.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HEU4717983.1"
                     /translation="MKTGAFTRRAALLITGLFAGIISHGQISDNFSDGDFTNNPVWSG
                     DSPAYIVNSSFQLQLNGTVADTSCLSFASPFVANAEWDFWVKMSFAPSDNNNCRVYLV
                     SDVANLKGPVNGYYIRLGENGSFDSVDLWEQSGTTSTKIIDGVNSHCAASTNTLRIKV
                     TRDATGTWNVFSDTLGGNNFQPEGTVFDNTHTTSNYCGVFSKYTVSNINKFYYDDFYV
                     GPVIVDTTPPNVISATATSSTTLDVLFDENVDPVTSQTPGNYSVDNSIGTPATAVRDA
                     ANHSLVHLAFGSSFVSATNYTLTVTNVQDLSANAVTTATASFLYLPLGNPSFHDVVIN
                     EIMADPSPVVGLPNTEFIELYNASSQNFDLNGWAFTDGSTTGSVNSHILPAGGYIILC
                     ANADTSLFSSFGPVTGVSSWPSLNNSGDNLKILDPSSNVIDSVNYDISWYHDASKQDG
                     GWTLELINPLASSFCSSSANWNASLNPAGGTPGAQNSVFNSSPDVTPPHPVSAYAQDS
                     LHVELCFSEPVNPALLTDVNNYAGLGTILSTHADSSTFSCVTLTLTTPLVNNTAYTVH
                     FSTLTDCAGNVVTPDTAGFSYHIIQPFDVLVDEIMADPDPPVQLPNEEYFELKNTTPY
                     LLKLDNWTITVGTTTKVLPAVTLPADSFVVLTTTAAAAQYIAINVVGVTSFPSLTNTG
                     SSITLRTPQGMVVHTVTYSDSWYQNSAKKNGGWSLEMIDPSNPCGGMSNWKASVSPWG
                     GTPGFPNSVNGPNADNTQPHLVRASIITADSIRLWFSEPLDSLTMSDITRYTIDHSLG
                     NPLSVKPLGPDFMKCDLKLSAPIAAGTTYYVSVNTSVSDCAGNTVIAPNGAPFALPQP
                     VAPNDIIINEILFDPNDGGTDFVEIYNRSQKVIDLKNMVLCSQDTINNVLTDLNVIAP
                     EGYLFFPGQYLVLSESGATVAMQYPVPTDPDRCLDMASIPAMNVSGDIVVLADTGFHI
                     IDRLVYSSSWHFPLLQNTKGVSLERIDFDRATQDPTNWHSASETVGFATPTQRNSEYN
                     PGENADDGAVVISTQVFSPDGDGYNDVVNINYNFSTPGYVANITIYDSRGRLVRTLVH
                     NEMLGTEEGSFSWDGTMDDRTKARVGAYIIYFEVFNSDGTVKQYKRTCVLAAKL"
     gene            4270..4800
                     /locus_tag="VFU15_09125"
     CDS             4270..4800
                     /locus_tag="VFU15_09125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717984.1"
                     /translation="MKKHFLFFFALFLALSAHSQLVTNNPDKDSDWGPPVKGKVRVYH
                     NSFNTKTDGSGFGFGYRRFTVDSVVNTETPFGLHYCNLKPYVSKDDKALAFLNKGIRW
                     EKNQHIAIGITIGASAALLTALLVHSPDNVNNAFLIGDAVCFGGVLTCNLFKRYYVYR
                     SISRWDDDLDKGLYRY"
     gene            4880..5503
                     /locus_tag="VFU15_09130"
     CDS             4880..5503
                     /locus_tag="VFU15_09130"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEU4717985.1"
                     /translation="MVKPKKEEASTEQQILEAARKIFREKGFDGARMQEIADEAKINK
                     AMLHYYFRSKDQLFRRIFVETMQSFLGTIVLLLNQPSSTWEEKIYAIAERYTDFLKSN
                     PGMPLFVMSELARSGHSVIEDLPVSAVVESSVFIRQLREEQRKKRVREIEPLQVIITI
                     ISGLVFPFVARPIIKTIGQFDDKKFNRFLEDRKIIVAEMLITYLRKK"
     gene            5510..6403
                     /locus_tag="VFU15_09135"
     CDS             5510..6403
                     /locus_tag="VFU15_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014680805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEU4717986.1"
                     /translation="MKTNLILSIAAAAFLFSCNSAEKKADAYGNFEAVETIVSAQGQG
                     QLLSFDVEEGQQLKVNENVGLIDTFALYLKKKEIDATIRSLNKKVPDSEAQLAVVRQQ
                     IENAMREKTRIENLVKADAAPSKGLDDINEQIAVLQQQEKALQSQLQTQSSTILSGGD
                     PLAVEKMQVDDLLAKCSIINPVSGTVTTKFAERGEVVAPGKPLYRIADLQNMILRAYV
                     SGDQLSKVKIGQHVKVSIDDGSGKMKTYDGTVEWISDKAEFTPKLIQTKEERVNLVYA
                     VKIRVVNDGAIKSGMPGEVNF"
     gene            6409..7320
                     /locus_tag="VFU15_09140"
     CDS             6409..7320
                     /locus_tag="VFU15_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020607366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU4717987.1"
                     /translation="MPAIDIDTICKSYGGVPAIENISFSVDKGELFGLIGPDGAGKTT
                     LFKILVTLLLPSAGKARVQGYDVVDDFLSIRQIIGYMPGKFSLYQDLSIEENLEFFAT
                     VFKTTVKENYDMIRDIYVQIEPFKKRRAGQLSGGMKQKLALCCALIHKPQVLFLDEPT
                     TGVDAVSRKEFWDMLQTLKAQGITILVSTAYMDEAALCDRIALIQHGKILSVNTNEGI
                     IAGYKKPLYAVKSGDTYRLLNDLRSLAATESCYPFGEYVHWTAKNEETGKPGLETYLA
                     QCSHRDVEVKRISPGIEDCFMDLMRKP"
     gene            7323..>8004
                     /locus_tag="VFU15_09145"
     CDS             7323..>8004
                     /locus_tag="VFU15_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU4717988.1"
                     /translation="MEKAVTVSKLTKKFGAFVAVNEISFEVDKGEIFGFLGANGAGKT
                     TAMRMLCGLLSPSSGEATVAGFDIYHEADEVKKNIGYMSQKFSLYEDLTVAENIRFFG
                     GIYGLNRKTVAERTDEILEKHALKDSRSTLVKSLPLGWKQKLSFSLALVHRPAIVFLD
                     EPTGGVDPVTRRQFWELIYEAAAGGTTLFVTTHYMDEAEYCNRVSIMVDGKIVALDTP
                     GGLRKSYEA"
BASE COUNT         1907 a         2310 c         2076 g         1711 t
ORIGIN      
        1 gggtccatcc gccatgcgga agaattgtcg atgacgatcg ttcccgcttc agcgaatttc
       61 ggcgcccact cgagcgacgt gcttccgccg gcggagaaca gcgcgatctg cggcttcatc
      121 gcgatcgctt tttccggcgt caccactttc catttttttc cgccgaatga aatttccttt
      181 cctgccgaac gctcggacgc gacaggaata agttcggtaa ccgggaaatt ccgctccgcg
      241 agaacttcca gcatttttgt gcccaccagg ccggtggcgc cgacaactgc gattttcata
      301 acgtggtttt ggttttgcaa atatccgtca gcggatgagt ttttgtttat ggtaaaggat
      361 aattgtctgg tttttttatc cgggcattgc agggacgcac gatcagcacg gacgtatgtg
      421 ttgtcatcct tctgaaaatc aaaaagcctt cccgtttccg gaaaggctct cgtttatttc
      481 gtttgcacaa acgcagatca gcctttccag cgaaggaagg gtttcttcat gcattttgcg
      541 gcaattgaat tcatggcggc aaagatgcaa tatttcagat gccggtgcaa cgggcttcac
      601 tttttctttt gctttcggac aggaaaaccg tcgcgattca tacagcccgg ctccggagca
      661 tcgatcgccg ggacctggct gccagccaaa ttctgacggc gctgccagcc cgttaacccc
      721 cgtgttttct ttctgctgaa aatttctccc gctgtgcgaa tcgtttatct ttacgtctca
      781 tgaaaacggg ggctttcaca cgacgcgcgg cattgctgat caccgggctt ttcgcgggaa
      841 ttatttccca cgggcagatc agcgataatt tttccgacgg cgatttcacc aataacccgg
      901 tatggtcggg cgattcaccg gcttacatcg tgaacagttc cttccagctg cagctcaacg
      961 gaaccgtcgc cgacacgtcc tgtctttctt tcgccagccc gttcgtcgcc aatgcggaat
     1021 gggacttctg ggtgaagatg agcttcgctc cttccgacaa caacaactgc cgcgtttacc
     1081 ttgtttccga cgtcgcaaac ctgaaaggac ccgtgaacgg ctattacatc cgcctcggcg
     1141 agaatggttc gttcgacagc gttgacctgt gggaacaaag cggaacgaca agcacgaaga
     1201 tcatcgacgg tgtgaacagc cattgcgcgg ccagcacgaa cacactgcgg ataaaagtca
     1261 cacgcgacgc caccggtacg tggaatgttt tttccgatac gctcggcggg aacaatttcc
     1321 aaccggaggg aaccgtgttc gacaacacgc atacgacgtc gaactattgc ggcgtgttca
     1381 gcaaatacac ggtgtcgaac atcaataaat tctattacga cgatttttac gtcggaccgg
     1441 tcatcgtcga cacgacacca ccgaacgtca tttccgcaac cgccacttct tccaccacgc
     1501 tcgacgtgct ctttgacgag aacgtcgatc cggtcacttc gcagacaccc ggcaactaca
     1561 gcgtggacaa cagcatcgga acgccggcca ctgccgtacg cgatgccgcc aaccattcgc
     1621 tggttcacct ggcattcggc agcagcttcg tgagcgcaac gaactacacg ctcaccgtga
     1681 cgaatgtgca ggacctgagc gcgaacgctg tcaccacggc caccgcgagc ttcctttacc
     1741 tgccgctggg aaatccttct ttccatgatg tcgtgatcaa cgagatcatg gcggatcctt
     1801 ccccggttgt cgggcttccg aatacggagt tcatcgagct gtacaatgcg agcagccaga
     1861 acttcgatct caacggctgg gcgttcaccg acggatcgac cacgggttcc gtcaattcac
     1921 acattcttcc ggcaggcggc tacatcattc tctgcgccaa tgcggacact tcgcttttct
     1981 cgtcattcgg acccgtcacc ggtgtttcgt cctggccttc gctgaataat tccggtgata
     2041 atctcaagat cctggatccg tcgagcaacg tgatcgattc cgtcaattat gatatttcct
     2101 ggtaccatga tgccagcaag caggacggcg gatggacgct cgagctgatc aacccgctcg
     2161 ccagcagctt ctgctcttcc tctgcgaact ggaacgcatc gctcaatccc gcaggcggaa
     2221 cgccgggcgc acagaacagc gtattcaaca gctcgccgga cgtgacaccg ccgcatccgg
     2281 tttccgctta tgcgcaggac agtcttcatg tcgagctttg cttcagcgaa ccggtcaacc
     2341 cggcccttct caccgacgtg aacaattacg ccgggctggg aaccattctt tccacgcatg
     2401 ccgacagttc cacgttttcc tgcgtgacgc ttacgcttac aacaccgctc gtgaacaata
     2461 ccgcgtacac cgttcatttt tccacgctca cggattgtgc aggcaacgtg gtcacgccgg
     2521 ataccgccgg cttctcctat cacatcatcc aaccgttcga tgtgctggtg gacgagatca
     2581 tggccgatcc cgatccgccg gtgcaactgc cgaacgaaga atatttcgag ctgaagaaca
     2641 caacgcctta cctgctgaag ctcgacaact ggacgatcac cgtcgggacg acaacaaaag
     2701 tgcttccggc cgtcacatta cctgccgaca gtttcgtcgt actgaccacc acggcagcag
     2761 cagcgcaata catcgccatc aatgtcgtcg gtgtgacgag cttcccttcg ctgaccaata
     2821 ccggctcctc gatcacgctg cgcacaccgc agggaatggt ggtgcacacc gtaacgtatt
     2881 ccgattcctg gtaccagaat tccgcgaaga agaacggggg ctggtcgctg gagatgatcg
     2941 atccttccaa tccctgcggc ggaatgtcga actggaaagc ttccgtttct ccctggggcg
     3001 gcacgcccgg ttttcccaac agcgtcaatg gcccgaacgc cgacaatacg cagccccatc
     3061 tcgtccgcgc atcgatcata accgcggata gtatccgtct ctggttttcc gaaccgctcg
     3121 acagcctcac gatgtcagac atcacccgtt acacgatcga tcacagcctg ggcaacccgc
     3181 tttcggtaaa accgctcgga cccgatttca tgaaatgcga tcttaagctt tccgcaccga
     3241 tcgccgcagg caccacgtat tacgtttccg tcaacacttc cgtttccgat tgcgcgggaa
     3301 atacggtgat cgctcccaac ggtgcgccgt tcgcattgcc gcagccggtt gcgccgaacg
     3361 acatcatcat caacgaaatt cttttcgatc cgaatgacgg aggaacggat ttcgtggaga
     3421 tctacaaccg ttcgcagaaa gtcatcgacc tgaaaaacat ggtgctctgt tcgcaggaca
     3481 cgatcaacaa cgtgctgact gatctcaacg tcattgcacc ggaaggatat cttttcttcc
     3541 ccgggcaata cctcgtgctg agcgaaagcg gtgcgacagt ggcgatgcaa tatcccgttc
     3601 ctacagaccc tgaccgctgc ctcgacatgg caagcattcc tgcgatgaac gtgagcggtg
     3661 acatcgtcgt tcttgcagat accggctttc acatcatcga ccgcctcgtg tattccagct
     3721 cctggcattt cccgctgctg cagaacacga aaggcgtttc gctcgagcgc atcgacttcg
     3781 accgtgccac gcaggatccc acgaactggc acagcgcttc tgaaactgtt ggcttcgcca
     3841 caccgacgca gcgcaactcc gaatacaacc cgggtgaaaa tgcagacgac ggggcggttg
     3901 tgatctccac gcaggtattt tctcccgacg gcgacggcta caacgatgtc gtgaacatca
     3961 attacaattt cagcaccccc ggttacgtgg cgaacatcac gatctacgat tcgcgcggaa
     4021 ggctcgtgcg tacgcttgtg cacaatgaaa tgcttggaac ggaagaagga tcattttcgt
     4081 gggacggcac gatggatgac cgcacgaaag cccgggtcgg cgcgtacatc atttatttcg
     4141 aggtcttcaa ttccgacgga acagtgaaac aatacaagcg cacgtgcgta cttgccgcga
     4201 aattgtaacc acttacaaaa ttttcagccc gtttgccggt cgtggcaaag ctcttgcagc
     4261 aatgcggtta tgaaaaaaca ctttctcttt tttttcgccc ttttccttgc actgtccgct
     4321 cactcgcagc tggtgacaaa caatcccgat aaagattccg actggggtcc gccggtaaaa
     4381 gggaaagtgc gcgtgtacca taattcgttc aacacgaaaa cggacggaag cgggttcggc
     4441 ttcggttacc gccgttttac ggtcgattcc gtggtcaata ccgagacgcc gttcggtctt
     4501 cattactgca acctgaagcc gtatgtaagc aaagacgaca aagcgctcgc cttcctcaac
     4561 aagggaatac gctgggaaaa gaaccagcac atcgcgatcg gcatcacgat cggcgcatct
     4621 gcggcactgc tcaccgcgct gctcgtccat tcgccggaca atgtgaacaa cgcttttctg
     4681 atcggcgacg cggtgtgctt cggcggcgtg cttacctgta atctcttcaa gcgctattac
     4741 gtgtaccgtt ccatttcgcg ctgggacgac gacctcgata agggtcttta ccgctattga
     4801 gctttccccg cttcctgaaa aaccatttgg aattccggga tctgttttgt tacctttgtt
     4861 aaccaaatgg ttaaatcgaa tggttaagcc aaaaaaagag gaggcttcca cggaacagca
     4921 gatactggaa gcggcgcgga aaattttccg tgaaaaggga ttcgacgggg cgcgcatgca
     4981 ggagatcgcc gatgaagcga agatcaacaa ggcgatgctg cattattatt tccgttcgaa
     5041 agaccagctg ttccggcgca ttttcgtgga aacgatgcag agctttctcg gcacgatcgt
     5101 gctgctgctc aaccagccgt catctacgtg ggaggaaaaa atatacgcca tcgcggaacg
     5161 ctacaccgat ttcctgaagt cgaacccggg gatgccgctc ttcgtcatga gcgagctggc
     5221 gcgcagcggg cattccgtga tcgaagatct cccggtttcc gcggtcgtcg agagctccgt
     5281 cttcatccgc cagctccgcg aagagcaaag aaaaaaacgc gtgcgggaga tcgagccgct
     5341 gcaggtgatc atcaccatca tttccggtct tgttttcccg ttcgtcgcgc gacccatcat
     5401 caaaaccatc gggcagttcg acgataaaaa attcaaccgc tttctcgagg accggaaaat
     5461 catcgtcgcc gaaatgctca tcacctatct ccgaaaaaaa taaatacgca tgaaaacgaa
     5521 cctcattctt tccattgccg cagccgcgtt ccttttctcc tgcaacagcg ctgaaaaaaa
     5581 ggcggacgca tacggaaact tcgaagccgt ggaaacaatc gtttccgcgc aggggcaggg
     5641 acaactgctt tcgttcgacg tcgaagaagg gcagcagctg aaggtgaacg agaacgtcgg
     5701 ccttatcgac acattcgcgc tttacctgaa gaaaaaagag atcgacgcga cgatccgttc
     5761 actgaataaa aaagtgccgg attccgaagc gcagctcgcc gtggtccgcc agcagatcga
     5821 aaatgcgatg cgggaaaaaa cgcgcatcga aaacctcgtg aaggcggacg ccgcgccgtc
     5881 gaaaggactc gacgacatca acgagcagat cgccgttctt cagcagcagg aaaaagcgct
     5941 gcaaagccag ctgcagacac agagcagcac gatcctttcc ggcggcgacc cgctggcggt
     6001 ggaaaaaatg caggtggacg acctgctcgc gaaatgcagc atcatcaacc cggtgagcgg
     6061 aaccgttacc acgaaattcg cggagcgcgg ggaagtcgtc gctccgggca aaccgctgta
     6121 caggatcgcc gacctgcaga acatgatcct gcgcgcttac gtttccggcg accagctttc
     6181 gaaagtgaag atcggccagc acgtaaaagt ttccatcgac gacggcagcg ggaaaatgaa
     6241 aacatacgac ggcaccgtcg agtggatcag cgacaaagcc gagttcacgc cgaagctgat
     6301 ccagaccaaa gaagaacgcg tcaacctcgt atacgcggtg aaaatacggg tggtcaacga
     6361 cggcgccatc aaatcgggca tgccgggtga agtgaatttc tgatcagcat gccggccatc
     6421 gacatcgata ctatctgcaa aagctacggc ggcgttcccg cgatcgaaaa tatttcgttc
     6481 agcgtggata aaggcgagct gttcggtctt atcggtcctg acggtgcggg aaaaacaacg
     6541 ctgttcaaga tcctcgttac gttgctgctt ccttccgccg gcaaagcgcg cgtgcagggt
     6601 tacgacgtgg tggacgattt cctttccatc cgccagatca tcggctacat gcccggaaaa
     6661 ttttcgcttt accaggacct cagcatcgaa gagaacctgg aatttttcgc caccgttttc
     6721 aaaacaacgg tgaaggaaaa ctacgacatg atccgcgaca tctatgtgca gatcgagccg
     6781 ttcaaaaaaa gacgcgcggg acagctgagc gggggaatga aacaaaagct cgcactgtgc
     6841 tgcgcgctca ttcacaaacc gcaggtctta ttcctcgacg aaccgacaac cggtgtggac
     6901 gccgtttcac gaaaggaatt ctgggacatg ctccaaacgc tgaaagcgca gggcatcacc
     6961 atcctcgttt ccaccgcgta catggacgaa gcggcattgt gcgaccgcat cgcgctgatc
     7021 cagcacggaa aaattctttc cgtgaacacg aacgaaggca ttatcgccgg gtataaaaaa
     7081 ccgctgtacg ccgtgaaatc gggcgacacg tacaggctgc tgaacgacct gcgcagtctc
     7141 gccgcaacgg aatcgtgtta cccgttcggc gaatatgtgc actggaccgc gaagaacgaa
     7201 gaaaccggca agcccgggct ggaaacttac ctcgcccaat gttcgcacag ggacgtggag
     7261 gtaaagcgga tttcaccggg catcgaagat tgcttcatgg accttatgcg gaaaccgtga
     7321 tcatggaaaa ggccgtcacc gtcagcaagc tcacaaaaaa attcggcgcc ttcgtcgcgg
     7381 tgaatgaaat ttcattcgaa gtggacaaag gtgaaatttt cgggttcctc ggtgcgaacg
     7441 gcgcaggaaa aacaacagcc atgcgcatgc tctgcgggct gctttccccc tcttccgggg
     7501 aagccaccgt ggcgggtttc gacatttacc acgaagccga tgaagtgaaa aagaacatcg
     7561 gctacatgag ccagaaattt tcgctgtacg aagacctgac tgtcgcggag aacatccgtt
     7621 tcttcggcgg catctacggg ctgaacagaa agaccgtcgc ggaacgcacg gatgaaattc
     7681 tcgaaaagca cgcgctgaaa gattcgcgga gcacgctggt gaaatcgctg ccgctcgggt
     7741 ggaaacaaaa actttcattt tccctggcgc tggtgcaccg tcccgcgatc gttttcctcg
     7801 acgagcccac gggcggcgtc gacccggtga cgcggcggca gttctgggag ctcatttacg
     7861 aagcggccgc gggcggaacg acgcttttcg tcaccacgca ttacatggat gaagcggaat
     7921 actgcaaccg cgtttcgatc atggtcgacg ggaaaatagt tgcgctcgat acgcccggcg
     7981 gattgcgcaa aagctacgag gcgg
//
LOCUS       DASTSF010000319         5462 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_551908,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000319 DASTSF010000000
VERSION     DASTSF010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5462
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_551908"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..664)
                     /locus_tag="VFU15_09150"
     CDS             complement(<1..664)
                     /locus_tag="VFU15_09150"
                     /inference="COORDINATES: protein motif:HMM:NF014851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 protein"
                     /protein_id="HEU4717989.1"
                     /translation="MVTLHAGAQPVSQNLDSGWQFSEQDKNEWMPATVPGTVHTDLMA
                     NKKIDDPFFSGNESKVQWVETKTWEYKTTFDCSEELWKQTHRELDFEGLDTYADVYLN
                     DSLLFTAEDMFLSYSFDVTKLLKKKSNVLRVVFHPASELIEKNRERSDFKNLPGGDRV
                     FIRKAQYQFGWDWGPRLVTCGIWKPVQLTGWSSTRIVSVNYRMISLGNDTAYVECDAN
                     LYA"
     gene            complement(772..1476)
                     /locus_tag="VFU15_09155"
     CDS             complement(772..1476)
                     /locus_tag="VFU15_09155"
                     /inference="COORDINATES: protein motif:HMM:NF015866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HEU4717990.1"
                     /translation="MKNILEIAAFSPEAAIIAANAGADRIELCSAYAEGGLTPSLSAM
                     EFVKQNVRIPVFIMIRPRGGDFVYSEIEKAVMKQDILHAKNTGADGIVLGVLTKENHV
                     DELLLKELVHLCSPLPVTFHRAFDLCPDPFEAMETLIRCGVKRILTSGQKATAEKGGG
                     TIAALHEKANGKIILMPGAGISPANIFALMKKTGCAEFHASAKSPGTPAGVFGFGENI
                     LPDAAIIAELKKKISL"
     gene            complement(1473..3869)
                     /locus_tag="VFU15_09160"
     CDS             complement(1473..3869)
                     /locus_tag="VFU15_09160"
                     /inference="COORDINATES: protein motif:HMM:NF012931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEU4717991.1"
                     /translation="MNSRPSSFHPAFLFRGKLRGEFSPARVLSFFIFTFLFLSSFAQY
                     DEQRYPVIPRPYKLLPFSGDFTLDSRTVIVTDMKSTQAETDFFVQQLKDIYNINLKHA
                     KKASKGNFIYVALDSSGVPPEGYQLFVNRNEIDLYGTKAGVFYGLQTLFQLIHVPAAV
                     DRLVLPTIPSCSVIDQPQFRWRGLHLDVSRHFFTKLQVKEYLRWMAMYKLNTFHWHLT
                     DDQGWRIEIKKYPRLTSIGAWRNGTLAGHEGAANSFIDTTEYGGFYTQNDIREIVRYA
                     DSLHITIVPEIEMPGHACAMLAAYPELGCTNGPFIVQPTWGVFNDVLCPSEKTFSFLD
                     NVLTEVAALFPGKFIHIGGDECPKAEWKTNDTCQVIIRNYHLKDEDELQSWFTKRIVA
                     MLQKKGKQAIGWDEILDGGLADGAAVMSWRGEEGGIAAAKAGHNVVMSPGGYCYFDHY
                     QSDAPGEPLAIGGYLPLEKVYSYRPVPPQLSADESKYILGVQANMWTEYIGDEKKLQY
                     MLFPRILALSEVAWSYTKENNYAFFLNRIDFHLNLFTRMNINCAKSIFDIKAKITSSM
                     VNGVALSLEPGLMTGHIRYYIGDSDTGTFDHDYDFPVIIDTTVTIHAALFEENEAVTR
                     TYVYHFDVSLATGKPVSLRKPPDKSYSTGGPFALVDGIRGEMPWKGSQWLGWWGDTLD
                     AVIDLGQDTLINSVTFNFLVDSSSWIYPAQNYTIGLSEDGKTYVPLDKNAWIVTGSAG
                     EFRNGGNVAGYARASSGSAKNQRARYIHLVVYPLGKIPPGNPGEGNPAWLFVSEINVN
                     "
     gene            complement(3871..4338)
                     /locus_tag="VFU15_09165"
     CDS             complement(3871..4338)
                     /locus_tag="VFU15_09165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717992.1"
                     /translation="MGSYFSYDDLTLLKGSENKQLKSVLYHFWQNKTNPSDFFEFTDK
                     VELHFSDGTKLVLSSTEDDEPAISVLKNFDAETSKLELLHRFGGKIDQRTEIMTSDPL
                     WKNVSGKTLAGVQLVDDGENTYRNDAVLLDFRSEKIEIRPGVEGLIIEPFEDV"
     gene            complement(4367..4711)
                     /locus_tag="VFU15_09170"
     CDS             complement(4367..4711)
                     /locus_tag="VFU15_09170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717993.1"
                     /translation="MLEGFDEEEDDVDEVEEVGVAVVVAGLAVAGDLAGFAVCVVFFG
                     ALGSCWWTWLTAADAAVTERANNAETKKVFIVLVLGEFFFLSHRKIIPGILLQMGNFG
                     DHGRKIAGNGKT"
     gene            complement(5227..>5462)
                     /locus_tag="VFU15_09175"
     CDS             complement(5227..>5462)
                     /locus_tag="VFU15_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070005 - cysteine-type aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HEU4717994.1"
                     /translation="AFDNLSTQDDHGMQITGQAKDQAGKKYFIVKNSWGTENNECGGY
                     FYCSEAYFQYKTTSILLNKSAIPKDIAKKMGIQ"
BASE COUNT         1199 a         1423 c         1494 g         1346 t
ORIGIN      
        1 ctgcgtaaag attggcgtcg cattccacgt atgccgtatc gttgcccagg ctgatcatgc
       61 ggtagttcac gctcacgatg cgcgtgctgc tccagccggt gagctgcacc ggtttccaga
      121 tgccgcaggt gacgaggcgc gggccccagt cccagccgaa ctggtattgc gccttgcgga
      181 tgaaaacgcg gtcgcctccg ggaagatttt tgaagtcgga acgttcgcgg tttttttcaa
      241 tgagctcgga tgcgggatgg aaaacaacgc gcaatacgtt gcttttcttt ttcaaaagct
      301 tcgtcacgtc gaagctgtac gaaaggaaca tgtcctcggc ggtgaagagg agtgaatcgt
      361 tgaggtaaac atccgcgtag gtgtcgagcc cttcgaagtc cagctccctg tgcgtttgct
      421 tccagagttc ttcgctgcaa tcgaatgtcg ttttgtattc ccatgttttc gtttcgaccc
      481 attgcacttt cgattcgttc ccgctgaaaa aaggatcgtc gatctttttg ttcgccatca
      541 ggtcggtgtg gaccgttccc ggaacagtgg cgggcatcca ttcgttctta tcctgctcgg
      601 aaaattgcca gccggaatcc aggttttgcg aaaccggttg cgccccggcg tgcagcgtga
      661 ccaaaacaaa aaacgccaga ccgcagatac gcgggagatg cagggttttc cctgaaggct
      721 tcgtgcattt ccctgtgcgt cttgatgccg ctgcggttga taggggtaat gtcataaact
      781 gatcttcttt tttagttctg caatgatcgc ggcatcggga agaatatttt caccgaagcc
      841 gaacacgcct gctggtgttc cgggactttt cgcggaagcg tggaattccg cgcagcccgt
      901 ttttttcatg agcgcaaaaa tattcgccgg ggagattccc gccccgggca tgaggattat
      961 ttttccgtta gctttttcat gaagcgcggc gatcgttccg ccgccttttt ccgcagtggc
     1021 tttttgtcct gaagtgagga tgcgcttcac gccgcagcgg atgagtgttt ccatcgcttc
     1081 aaacggatcg gggcacaggt cgaacgcacg gtgaaaggtg accggaagcg ggctgcagag
     1141 atgaacgagc tccttcagca gcagttcgtc aacgtggttc tctttcgtca gcacgccgag
     1201 cacgatgccg tccgcgcctg tattttttgc gtgaagaata tcctgcttca tgaccgcctt
     1261 ttcaatttcg ctgtaaacaa aatcaccgcc acgcgggcgt atcatgatga aaacgggaat
     1321 gcgcacattt tgttttacga attccatggc gctgagcgaa ggcgtgagtc ctccttcggc
     1381 atacgcgctg cacagctcga tgcggtccgc acctgcattc gcggcgatga tcgcagcttc
     1441 cgggctgaac gcggctattt caagaatatt tttcaattca cgttgatctc tgatacgaaa
     1501 agccaggccg ggtttccttc acccggattt cccgggggga tttttcccag cggataaacc
     1561 acaagatgaa tataccgcgc ccgctgattt ttagctgaac cggaagaagc tctcgcatag
     1621 ccggccacgt tcccgccgtt ccggaattca ccggcgctgc cggtcacgat ccatgcgttt
     1681 ttgtccaacg gaacgtacgt tttcccgtct tcggaaagcc cgatcgtata attctgcgca
     1741 ggatagatcc agctcgatga atcgacgaga aaattgaacg tcacggaatt tatcagcgtg
     1801 tcctgcccga gatcgatcac tgcgtcgagc gtatcgcccc accagccgag ccattgcgaa
     1861 cctttccagg gcatttctcc cctgatgccg tcgacgagcg cgaaaggccc gccggtgctg
     1921 tagcttttgt ccggcggttt gcgcaatgag accggttttc ctgtcgccag cgaaacatcg
     1981 aaatgataaa cgtaggtccg cgtgaccgcc tcattttctt caaagagagc cgcatggatc
     2041 gtcactgttg tgtctatgat gacggggaaa tcgtaatcat ggtcgaacgt gcccgtgtct
     2101 gaatcgccga tgtaatagcg gatgtgcccc gtcataagcc cgggctcaag tgaaagcgcc
     2161 acgccgttca ccatcgaaga agtgatcttc gctttgatgt cgaaaatgct tttcgcgcag
     2221 ttgatattca tgcgcgtaaa aagattgagg tgaaaatcga tgcggttgag gaagaatgca
     2281 tagttgtttt ccttcgtgta cgaccacgca acttccgaca gcgcaaggat gcgcgggaaa
     2341 agcatgtact gcaatttttt ttcatcgccg atgtattccg tccacatgtt cgcctgcacg
     2401 ccgagaatgt atttcgattc gtctgcggaa agctgcggag gaaccggccg gtagctgtat
     2461 actttttcca gcggaaggta accgcctatc gcgagcggtt ctcccggcgc gtcggactgg
     2521 tagtgatcga aataacaata cccgcccggc gacatcacga cgttgtgtcc cgcttttgct
     2581 gcggcgatcc cgccttcttc accgcgccag gacatgacgg ctgcaccgtc ggcaagtccg
     2641 ccgtcgagga tctcatccca tccgatcgcc tgtttccctt ttttctgcag catggcgacg
     2701 atgcgtttcg tgaaccagct ctgcagctca tcttcatctt tcaggtgata attgcggatg
     2761 atcacctggc acgtgtcgtt cgttttccat tccgctttcg ggcattcatc accaccgatg
     2821 tgaatgaatt ttccggggaa cagggcggca acttccgtga gcacgttgtc gagaaaagaa
     2881 aatgtttttt ctgaaggaca cagcacgtcg ttgaaaacgc cccacgtggg ctgaacgatg
     2941 aagggaccgt tcgtacagcc gagttcggga taagcggcga gcatagcgca cgcatgtccc
     3001 ggcatttcaa tttcgggaac gatggtaatg tgcagggaat cggcataacg cacgatctcg
     3061 cggatgtcgt tttgcgtata gaaaccgccg tattcagttg tgtcgatgaa agagtttgca
     3121 gcaccttcat gcccggcaag tgttccgttc cgccacgcgc cgatggaagt aagccgggga
     3181 tattttttta tttcaatgcg ccagccctgg tcgtcggtga ggtgccagtg gaaggtgttc
     3241 agcttgtaca tcgccatcca gcgcaggtat tctttcacct gcagcttggt aaaaaaatga
     3301 cgggaaacat cgaggtgaag tccgcgccac cggaactgcg gctggtcgat gacggagcag
     3361 gatggaatag tgggaagcac gagcctgtcg accgcggcag gaacgtggat gagctggaaa
     3421 agcgtctgca ggccgtagaa gacaccggct ttggtcccgt aaagatcgat ctcgttgcgg
     3481 ttgacgaaaa gctggtagcc ttccggcggt acacccgacg agtcgagcgc gacgtaaatg
     3541 aaatttcctt tcgacgcttt cttcgcgtgt ttaagattga tgttgtaaat gtctttcagc
     3601 tgctgaacga aaaaatccgt ttccgcctgc gtgcttttca tgtcagtgac gatgacggtc
     3661 cgggaatcga gcgtgaaatc gccggaaaaa ggaagcagct tgtaaggccg gggaataaca
     3721 gggtagcgtt gctcgtcgta ttgtgcgaaa gaagaaagga aaagaaaagt gaaaatgaaa
     3781 aaggagagaa cgcgtgcagg agagaattcc ccgcggagtt ttccgcggaa aagaaatgca
     3841 ggatggaaag acgacggacg ggaattcatc tcatacatct tcgaacggtt cgatgatgag
     3901 tccttccacg ccgggacgga tctcgatctt ttcactgcgg aaatcgagaa ggacggcatc
     3961 gttacggtat gtgttttccc cgtcgtcgac gagctgcaca ccggcgagcg ttttcccgct
     4021 gacgttcttc cagagcggat cgcttgtcat gatctccgtc cgctgatcga tcttcccgcc
     4081 gaaacggtgg agcaattcca gcttgctggt ttccgcgtcg aaattcttca gcacgctgat
     4141 cgcgggctcg tcgtcttcgg tggaagaaag cacgagcttc gttccgtcgg aaaaatgcaa
     4201 ttccacttta tcggtgaatt cgaagaaatc ggacgggttg gttttgttct gccagaaatg
     4261 atacagcacc gatttcagct gcttgttttc gctgcctttc agaagcgtga gatcgtcgta
     4321 cgagaagtag ctgcccatgc tgtaaagtta aaaagtgtga agcgatctac gttttgccgt
     4381 tcccggcgat cttcctgccg tggtcgccga aattccccat ttgcagcaag ataccgggaa
     4441 taattttacg gtgtgataaa aagaaaaatt cacccaaaac aagaactatg aaaacctttt
     4501 ttgtttcagc attatttgct ctttccgtga cagctgcatc ggccgctgtc aaccatgtcc
     4561 accagcagga tcccaaggcg ccgaagaaaa cgacgcagac ggcaaagccg gccaaatcac
     4621 cggctactgc caaaccggct accaccacag ccacgccgac ctcttcaact tcgtcaacgt
     4681 catcctcttc ttcgtcgaag ccttccagca tgcgcacgca caagcctgca ggccatgccg
     4741 gggcaaaacc ggccgcaacg ccgaagacgg attcgcattc ggggcagtga ggggtgtcag
     4801 ccttcggtac gtcacgcgca gcgtgactac tcagtctgac gatggagtgt gtcagccttc
     4861 ggtacgtcac gcggagcgtg actactcagt ctgacgatgg agtgtgtcag ccttcgatac
     4921 gtcacgcgca gcgtgactac tcagtctgac gatggagtgt gtcagccttc gatacgtcac
     4981 gctacgcgtg actactcagt ctgacgatgg agtgtgtcag ccttcggtac gtcacgcgga
     5041 gcgtgactac tcagtctgac tgtcggtgtg cgatacgcag cgtgactact cagtctgact
     5101 gttggtgtgc gatacgcagc gtgactgctc agtccaaaca gcaataaaaa aaccgcagaa
     5161 agttctgcgg tttttttgat gagctgtttt ttcgccgggc tgagtgcgaa gcccgtaggg
     5221 aaatgattac tgtatcccca ttttcttcgc gatgtctttg ggaatagcgc tcttgttgag
     5281 aaggatgctc gtggtcttgt actggaaata cgcttcggag cagtagaaat agccgccgca
     5341 ttcgttgttt tccgtgcccc aggaattttt cacgatgaaa tattttttgc ccgcctggtc
     5401 tttcgcctgc ccggtaatct gcatgccgtg atcatcctgc gtgctcaggt tgtcgaatgc
     5461 tt
//
LOCUS       DASTSF010000320         2357 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_901620,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000320 DASTSF010000000
VERSION     DASTSF010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2357
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_901620"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2357
                     /locus_tag="VFU15_09180"
     CDS             <1..>2357
                     /locus_tag="VFU15_09180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4717995.1"
                     /translation="ANRNLGEDFFRYQAVKVDEIALCADIDIANDAVVEEVEAEILHA
                     VEKFLDPAVTFYSLSEMYAKGYTTDQIFNGPLLQHGFIDDKELALAEKRKVIHVSDLI
                     SIIMDIPGVIAVRKIQIAGIPLNNVDNIPVETVKWCLEIPIEKNYIARLDIERSKLLF
                     YKGELPYRANDVTVQQLLDQLEASDRPQKIQNPDYDIHLTPGEYKEIENYCSIQEELP
                     LVYGTGSPGLPATATVQRQAQAKQLKGYLLFYDQLLADYLSQLFHVKDLFSMNDALDT
                     DGNPVIDKTYFSQSLVNIVPDALPLYNDPVNDATIVQAFTEDRATYLQRRNRFLDHLM
                     ARFAESFNDYAMLVYKIDGPKAPDELIADKLAFLNNYPLISSARDTGFNYKAPCNLWS
                     IDNISGLERRASYLCGVDRPLPSALSFSVNFDIRVSGDPDEFYFAVLDNSVSRDLVYS
                     PGATATGFPSTDDARVALESFIDIALTPENFVMYNGNNDVITDPLVPGPDPYRFEVVC
                     DGKLAGINAQSYGAGDFPTMQADITTALQIIYDEFFNNPESNRYNLECFMDKYLDVTG
                     PTFPAPPKPFCPPQYDYAFTLKDGDLVTSTSLLTGTVKGLYQEFDPPVPVGDQAAENK
                     ERLLMDMLRDASDIRNYRYAFDPSNPMKEVFNVVDRCSDIIGTSFEDDFNQNIQDILI
                     AIQGLAPTLNQFTVVDSTGNDGTYTANTISIDLTNAQRLVFTPNEPINSATGDGFLRF
                     TINDNFLTLVPTVIDANTGDNFFEVNKSLSRVIFPGETIHIASGP"
BASE COUNT          570 a          692 c          565 g          530 t
ORIGIN      
        1 tggcgaaccg caatctcggc gaggatttct tccggtacca ggccgtgaaa gtggatgaga
       61 tcgccctttg cgcagacatc gatattgcca atgatgcagt ggtggaagaa gtggaagcgg
      121 aaattctcca cgccgtggaa aaattcctcg atccggccgt gacgttctac agcctttctg
      181 aaatgtacgc gaaaggctac accaccgacc agatcttcaa cggtccgctc ctgcagcacg
      241 gtttcattga cgacaaagaa ttagcgctcg ccgagaaacg aaaagtaatt cacgtttccg
      301 atctcatcag catcataatg gatatccccg gtgtgatcgc tgtgcgcaag atccagatcg
      361 ccggcattcc cctcaataac gtcgataaca tcccggtgga aaccgtcaaa tggtgtttgg
      421 aaattcctat tgaaaaaaat tacatcgcgc gcctcgacat cgaacggtca aagctgctct
      481 tttacaaagg cgagcttccc taccgtgcga acgacgtaac agtgcagcag ctgctcgacc
      541 agcttgaagc aagcgaccgt ccgcagaaga tccagaaccc ggattacgac attcacctta
      601 caccgggcga atacaaggaa attgaaaatt attgcagcat acaggaagaa cttccgctgg
      661 tttacggcac aggctctccc gggcttcctg ccacggcaac tgtgcagcgg caggcgcagg
      721 cgaaacagct gaagggatat cttcttttct acgatcagct gctggctgat tatctctcgc
      781 aattgttcca tgtgaaagat ctcttttcca tgaacgacgc gctggatacc gacggcaatc
      841 ctgtgatcga caaaacgtat ttctcacagt cgctcgtcaa catcgttcct gacgcgctcc
      901 cgctctataa cgatccggta aatgacgcga cgatcgtgca ggcctttacc gaagaccgcg
      961 ccacgtatct ccagcgccgc aaccgtttcc tcgatcacct gatggcgcgc ttcgccgaat
     1021 cgttcaatga ttatgcgatg ctcgtgtaca agatcgacgg accgaaagcg ccggatgagc
     1081 tcattgccga caagctggca ttcctgaata attatccgct catcagctcc gcacgcgata
     1141 cgggtttcaa ctacaaggcg ccgtgcaatc tctggagcat cgacaatatt tccggtctcg
     1201 aacgccgcgc ttcttatctc tgcggggtcg atcgcccgct tccttccgcg ctttccttca
     1261 gcgtcaactt cgatatccgc gtttcaggag atccggacga attttatttt gccgtcctcg
     1321 acaactccgt ttcgcgcgat ctcgtttaca gcccgggagc aacggccacc ggcttccctt
     1381 ccaccgacga tgcacgcgta gcgcttgaat cctttatcga tatcgcgctt acgcccgaaa
     1441 atttcgtgat gtacaacggc aacaacgatg tcatcacgga tcctcttgtc cctggtcctg
     1501 atccataccg cttcgaagtg gtatgcgatg gaaaacttgc cgggatcaat gcgcagtctt
     1561 acggtgcagg agattttccg acgatgcagg cggatatcac cactgcactg cagatcattt
     1621 acgacgagtt cttcaataac ccggaatcga accgttacaa cctcgagtgc ttcatggaca
     1681 agtacctcga tgtaaccggt ccgacgttcc ctgcgccgcc gaaacctttc tgtccgccgc
     1741 aatatgatta tgccttcacg ctgaaagacg gggacctcgt tacatcgact tccctgctga
     1801 ccggcacggt gaaaggactt taccaggaat tcgatccgcc tgtaccggtc ggcgaccagg
     1861 ccgcagaaaa taaagagcgc ctgctgatgg atatgctgcg cgatgcgtcc gacatccgca
     1921 attaccgtta cgcattcgat ccgtcgaacc cgatgaaaga agtgttcaac gtcgtggatc
     1981 gctgcagcga tataatcggc acgagcttcg aagacgattt caaccagaac atccaggaca
     2041 tcctgatcgc catccaggga cttgcgccga cactgaacca gtttaccgtg gtggattcca
     2101 caggaaacga cggcacatac acggcgaaca cgatctccat tgatctcacc aacgcgcagc
     2161 gtctcgtttt cacgccgaat gaacccatca attcggcaac cggcgacggc ttcctgcgtt
     2221 tcacgatcaa cgacaatttc ctgacgctgg tgcccactgt tatcgatgcg aatacaggcg
     2281 acaacttttt cgaagtgaac aagagcctgt cgcgcgttat tttccccggc gaaacgatcc
     2341 acattgcgag tggcccc
//
LOCUS       DASTSF010000321         2554 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1157478,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000321 DASTSF010000000
VERSION     DASTSF010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2554
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1157478"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1168)
                     /locus_tag="VFU15_09185"
     CDS             complement(<1..1168)
                     /locus_tag="VFU15_09185"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019598955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEU4717996.1"
                     /translation="MSKLKYERPAIKKLNAGLMNKFGTRSEFEPVKHIDGVPVKSIIE
                     QYGSPVFVLSERQIRRNYRNAVRAFKTRYPKVQFAWSYKTNYMNAVCKVFHQEGSWGE
                     VVSGFEYQKALRNGVPGNRIIFNGPDKSEADLRSAIANDSLIHIDHFDELFFLVELAD
                     ELKKKPRVAIRVNMDTGVYPMWDRFGFNYENGQAWNAIQKIAGSGKLNLVGLHAHIGT
                     FMLTPSAYAVAASKLCDLAWHCSTQLNNPITYIDLGGGFPSQNTLKGSFLPGTDTVPP
                     IDEFAEAITTAILAFGFRREDLPLLILETGRALIDDAGYLLGSVLATKRLSDGRRATI
                     MDFGVNVLFTSFWYEHKISPAQDFSHHNEDQVLFGPLCMNIDVVRESVNLPLLNK"
     gene            complement(1241..2317)
                     /locus_tag="VFU15_09190"
     CDS             complement(1241..2317)
                     /locus_tag="VFU15_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012500435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEU4717997.1"
                     /translation="MAKRTKSARPRYTVAVTGLNAIDSPGPGVAVIRAIREANDYDVK
                     IIGLSYEALEPGIYMDNVTDRTYQVPYPAAGSEALMKRLEYIHDIEKIDVLIPNFDAE
                     LHNFIRLAPRLKSMGISTFLPTMEQFEARDKLNLFAFGKKHGLFVPEDRIIFRAEELA
                     DAAKAFGYPLVVKGKFYDAVVSQTAEQAQKAFHKLHAKWGLPIIVQQFISGSEVNIAA
                     LGDGKGNAVSIIPMRKLYITDKGKAWAGITLDDPSLIALAKKFMKATNWRGGLELEIM
                     RTVDGKPYIMEINPRFPAWIYLSAGAGQNQPAALVKLALGQEVEPYTDYHVGKMFIRY
                     SWDQIVDIESFQQISAFGEFANSK"
     gene            complement(2339..>2554)
                     /locus_tag="VFU15_09195"
     CDS             complement(2339..>2554)
                     /locus_tag="VFU15_09195"
                     /inference="COORDINATES: protein motif:HMM:NF017239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HEU4717998.1"
                     /translation="SETGFIFNPSTGDSFSTNPVAAEIILLMKEGKSISQVKESILSK
                     YEVDKISLEKDVDDLLTQLKDHNLLEA"
BASE COUNT          536 a          675 c          723 g          620 t
ORIGIN      
        1 ccttgttgag cagcggaaga ttcacgctct cgcgcaccac atcaatgttc atgcacagcg
       61 gaccgaacag cacctggtct tcgttgtgat gcgaaaaatc ctgcgcggga ctgatcttgt
      121 gctcgtacca gaaggaagtg aacagcacgt tcacaccaaa atccatgatc gttgcacgcc
      181 gtccgtccga caagcgcttc gtcgcgagca cggagccgag caggtagcct gcgtcgtcga
      241 tgagcgcgcg cccggtttcg agaatgagca acgggagatc ttcacggcgg aaaccgaatg
      301 caagaatggc ggtggtgatc gcttctgcaa attcgtcaat ggggggaacc gtgtccgttc
      361 cgggaaggaa agaacctttg agcgtgttct gcgaaggaaa tccgccgccg agatcgatgt
      421 acgtgatcgg gttgttcagc tgcgtggagc aatgccaagc gagatcgcag agcttcgaag
      481 cggccaccgc gtaagcggaa ggcgtgagca tgaacgtgcc gatgtgcgca tgcagcccca
      541 cgagattcag cttgcccgaa ccggcgatct tctggatcgc gttccacgcc tgcccgttct
      601 cgtaattgaa gccgaagcgg tcccacatcg ggtaaacgcc ggtatccatg ttgacgcgga
      661 tggcgacgcg cggtttcttc ttcagctcat cagccagctc gacgaggaaa aacaattcat
      721 caaaatgatc gatatgaatg agcgaatcgt tggcgatcgc gctgcggaga tccgcttccg
      781 atttatcagg tccgttgaaa ataatgcggt tgcccggaac accgttgcgc aatgctttct
      841 ggtattcgaa accggaaacc acttcgcccc acgagccttc ctggtggaac actttgcaca
      901 cggcgttcat gtaattcgtt ttgtacgacc acgcgaactg cactttcggg taacgcgttt
      961 tgaatgcgcg caccgcgttg cggtaattgc gccggatctg gcgttccgaa aggacaaaca
     1021 ccgggcttcc gtattgttca atgatggatt ttacgggaac gccatcgatg tgcttcaccg
     1081 gttcgaattc cgaacgcgtt ccgaatttgt tcatgagacc ggcgttcagc ttcttgatcg
     1141 cgggccgttc gtatttcagt tttgacatgg ccatggtgtg ttattaagtt ggaaaggttg
     1201 aaaagttggg aagttggaag tgataccggg gaatgatcgg ttatttacta tttgcgaatt
     1261 ccccgaacgc cgagatctgc tggaacgatt cgatgtccac gatctggtcc cacgaatagc
     1321 ggatgaacat tttcccgacg tggtaatccg tgtacggttc cacttcctgg ccgagcgcga
     1381 gctttacgag cgcggccggc tggttttgcc cggcgccagc cgacaggtag atccaggccg
     1441 gaaaacgcgg attgatctcc atgatgtacg gttttccgtc gacggtgcgc atgatctcca
     1501 gttcgagccc gccgcgccag ttcgtcgcct tcatgaattt tttcgcgagc gcgatgagcg
     1561 aaggatcgtc gagcgtgatg ccggcccacg ctttcccttt atccgtgatg tagagcttgc
     1621 gcatgggaat gatgcttacc gcgtttcctt tcccgtcgcc cagtgcggcg atgttcactt
     1681 ccgatccgga gatgaactgc tgcacgatga tcggcaatcc ccatttcgcg tgcagcttgt
     1741 ggaacgcttt ctgcgcctgt tccgccgtct gcgaaaccac ggcatcgtaa aattttcctt
     1801 tcaccacgag cgggtagccg aacgctttcg ccgcatcggc cagctcttcc gcccggaaaa
     1861 tgatgcggtc ttcggggacg aagagcccgt gcttttttcc aaatgcaaaa aggttcagct
     1921 tgtcgcgcgc ttcgaattgc tccatcgtgg gaaggaacgt cgagatgccc atcgatttca
     1981 ggcgcggcgc aagccggatg aaattgtgca gctccgcatc gaaattggga ataagcacgt
     2041 cgatcttctc gatgtcgtga atgtattcga gacgtttcat cagcgcttcg cttccggccg
     2101 ccgggtacgg gacctggtaa gtgcggtcgg tgacgttgtc catgtaaatg ccgggttcca
     2161 gcgcttcata ggaaagaccg atgatcttca cgtcgtaatc gttcgcttca cggatggcgc
     2221 gtatcaccgc cacacccgga ccgggactgt cgatcgcgtt gagtcccgtt acggccacgg
     2281 tatagcgcgg ccgtgcgctt tttgttcgct ttgccataac aatacgtttt tgtgtggatt
     2341 acgcttcgag aagattgtga tctttcagct gcgtgagcag atcgtcgaca tctttttcga
     2401 gagagatttt gtcgacttca tatttcgaaa gaatggattc tttcacctgc gaaatggatt
     2461 ttccttcttt cataaggagg ataatctctg cggcgaccgg gttagtggaa aaggaatccc
     2521 ctgtcgaggg attgaagatg aacccggtct cgct
//
LOCUS       DASTSF010000322        15197 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1798317,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000322 DASTSF010000000
VERSION     DASTSF010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 15197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15197
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1798317"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(72..1976)
                     /locus_tag="VFU15_09200"
     CDS             complement(72..1976)
                     /locus_tag="VFU15_09200"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007652428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HEU4717999.1"
                     /translation="MQEPVSILKQHWGFDSFRPGQAEIIQSVLDGNDTLALLPTGGGK
                     SICFQVPALCKEGLCIVVSPLIALMQDQVENLKRRNIPAMAITSAMGKREQDAAFDTC
                     VYGKIKFLYLSPERLQTELARVRIAKMKVNLIAVDEAHCISQWGYDFRPPYLLIGELR
                     ELLPGVPVIALTATATEKVVEDIQEKLLFKKKSVFRNSFARTNLAYIVKKTEDKNSYL
                     LKLSKQVDGSGIIYVRNRRKTQEIASFLYKNGIPVTFYHAGLAAQERQARQQQWLNNQ
                     AKVMVSTNAFGMGIDKPDVRFVAHVDLPDSPEAYFQEAGRAGRDGKDSGAILLWNQHD
                     LSELDRNFESSFPPQEEIRRTYQAIANLYQVPVGAGEGATFVLDQKKICDTYKLDPLN
                     VFNSLKILEKEGYLAVNDAVYTPSRLKFISDNETLYRFQVANAYYETIIKTILRMYPG
                     LFDEYIRIREKEIANKTGVSEEKVIRMLHELEQKGLLNYVPQSDKPLLTFITRRADAK
                     TLQISPEHLAQRKKTAGERIAAMKRFISDERTCRQRTLLAYFGETGAPDCGKCDVCKK
                     RSGKNKRTDRDRLALAIIDALREQPRSIRELPDLFPDESQEELNFIVRQLLDAERVRF
                     TRDHTLEVAG"
     gene            complement(2023..2772)
                     /locus_tag="VFU15_09205"
     CDS             complement(2023..2772)
                     /locus_tag="VFU15_09205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718000.1"
                     /translation="MIISRAGSAQPTVPRNDFSDGGDEGEAAAWDYDELRKPEKALQF
                     MFYESSDLGMNFEGMDSVAANVLIAEDHFTSLKNTEMLIEDDAYRGGSSGNCACFYVL
                     VRKDSTGNAAIILSGTSGSFGRSAVKDITGDGIPEIVTECGITWMGDDNGYFTIFNFK
                     NGKKNELFSDVSYARVNSGKSDHYDNLKKGDTISVEATDSILGFSGPGHGAVMSIRTI
                     KTYNGGTSVDEIFRNAVLMRDTETTLLGDGK"
     gene            complement(2869..3492)
                     /locus_tag="VFU15_09210"
     CDS             complement(2869..3492)
                     /locus_tag="VFU15_09210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718001.1"
                     /translation="MKSAPALLAFFVLLQFSLRAQDTTHVTTHYHNGKVNEQFTQNKK
                     GDKEGNYLRYSTHGKTLVTGQYKDGVPVGVWNYYSSDTSGTLVDRLDFDTHKELFVDS
                     LRVPQLICGPRYFGGNLAKNEYIAHRIRTDFTPNEIAPYHGQSFTVTFAIDTTTMKPV
                     MPVLDESGLPSSVVEKMKQIVLDMPAWLPPVCKDKNEVWQFSVVFTF"
     gene            complement(3550..6825)
                     /locus_tag="VFU15_09215"
     CDS             complement(3550..6825)
                     /locus_tag="VFU15_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020404031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU4718002.1"
                     /translation="MKYSIFFLSAVAAAAVFFTAADPAKPKKWDVNNPPGDYKDVTFT
                     VNEGTWMNLDVSPDGKTVAFDLLGDIYSIPITGGTAVPLQTGHAWQCQPRYSPDGKKI
                     CFTSDEGGGDNVWVMNADGSGAKEVTNENFRLLNNGVWSADGQYIIARKHFTSTRSLG
                     AGELWMYHFTGGDGVQLTQRKNQQQDLNEPSVSADGKYVYYSEDMYPGGYFQYNKDPN
                     NQIYAIRRYNFTTGEIEDVTGGPGGACRPQVSHNGKLLAFVRRVREKSVLFLRDLATG
                     EEWPVYDDLSKDQQEAWCIFGVYTGFSWTPDDKHVVIWAKGKLKNIDVATQKVADIPF
                     SVTCHHHIYNALRFQQDVAPDQFTAKMIRNAVTSPDEKTLVFNAAGFLWKKTLPDGKP
                     ERLTDETDFEFEPAFSRDGSKLAFVSWNDEQTGAVMTLDMKSPKAKPVKITNEKGIFR
                     TPAFSPDGNTITFWKETGNDHQGYTFCVNPGIYTVPAAGGNATYLFNDNAQKPTFSAD
                     GKEIFYYVDNGDSKELKAYDPDKKTARSVATSNYAWEIVPSPDNKWIAFRELFKVYVC
                     AFPPSGKTLELANNMSQVPMQCITRDAGQSIDWSADSKKIHWTMGERYFTREIKNTFD
                     FVPGAPDSLPGMDTTGIKINLVLQSDKPKGAVAFTNARIITMDANNSVIENGCVLVRE
                     NRIEEVGPMGNVKIPGDAKVIDCTGKTIMPGLVDVHSHMGTFRQGLSPQKQWSYYANL
                     AFGVTTTHDPSSNTEMVFSQSEAVKAGNMVGPRIFSTGWILYGAEGDFKAVINNYNDA
                     RSAVYRTQACGAFSVKSYNQPRREQRQQIITAARNLKMMVVPEGGSFFYHNMTMILDG
                     HTGIEHNIPVAPVYDDVVKLWSNSETGYTPTLIVTYGAMNGECYWYQHSNVWENSRLL
                     NFYPRGMIDSRSRHRVMAPEEEYNNGHILVSQSVKKLADAGVRVNLGAHGQLQGLGAH
                     WELWMLAQGGMSPMQALRCATMNGAWYLGLDKSIGSLEKGKLADLIVLDNNPLEDIYN
                     SQYVKYTMVNGRLYDSQTMNEIGNYDRKHTKFWWENPKYFPNYTWHEDTNSFMEDACE
                     DDN"
     gene            complement(6914..7387)
                     /locus_tag="VFU15_09220"
     CDS             complement(6914..7387)
                     /locus_tag="VFU15_09220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718003.1"
                     /translation="MKKLPVYIMLAAMSGLALPLPSQAPCVTYHRQAGCSQASEDGFL
                     YNSQSKSGLFARGTTSKLKAVFYSGFDYSISMCADKLLGTGIGMVLSDATTGEVLYDN
                     ATDNKATHFEFSNETTRNMFITITIPGSGPGKGKSADAACLGILIEQKVTPKVGF"
     gene            complement(7419..8327)
                     /locus_tag="VFU15_09225"
     CDS             complement(7419..8327)
                     /locus_tag="VFU15_09225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718004.1"
                     /translation="MKKRFWPALLSVPVAVLALTLNSCNNDNSDKNGVDTMGDTTSAV
                     EKKDTIGNQEFAQVPSPSEMFAFMKAAAGGNASADVLNPVENEKKYESQKAQSLNLGV
                     YSADLLYCSTFSVSNKVVPYFGTCMRMGDKLKVATDISDKDKDRISKNAGNADSLVAI
                     SNDLYLTTFENLDQNQRGDDLGLMLAGGWVESLYLMTNMVKDFDKDKATVDGVVQQKG
                     SLDNLVDYLSKHESNPDVKAVSDQLKELKALFDAVPVSTTASGMTMKNGKHVLGGGSK
                     PTPTKDQFEKIKSKVADIRNGFISAQ"
     gene            complement(8682..8755)
                     /locus_tag="VFU15_09230"
     tRNA            complement(8682..8755)
                     /locus_tag="VFU15_09230"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8719..8721),aa:Pro,seq:cgg)
     gene            8958..9689
                     /locus_tag="VFU15_09235"
     CDS             8958..9689
                     /locus_tag="VFU15_09235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718005.1"
                     /translation="MRKAILGLSCFMMIISSLRSQVVVQGIVPGNNPKPAHFHPCVNF
                     GLYIPYAVINNRLEVRGSYKPGISLMTGFQRHRWFMWTAEYSRYFTHTASPSFDNIKA
                     WNAEMNGIFLMNVGESYVIFRAIFGASLLDWNGRFIGPTLNDNNKWSIGMNIHQSWIA
                     ANLGCGFEYNICHGLIASADYRARFASKNGDLISISDTAYFIGLRYRFGNNENDADSG
                     SGARRKSSTSKPGRIYKWLRHRPSA"
     gene            9662..10447
                     /locus_tag="VFU15_09240"
     CDS             9662..10447
                     /locus_tag="VFU15_09240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718006.1"
                     /translation="MAQAPSVSMMFRRIAGSNRGKIFFFLAAFVLLGSFSPAQTATVT
                     FSGKVFDKRDSSATPAPIVVNKRTYTGSTGSMDGSFSITGLKTDTFIVTASGYELQRF
                     CFRDSTAKDTFRLRIGLTIKSTTLQAVAIYPVKDLDAIKKERESLGVQQTYMTSGITD
                     AVQSPITYMYERFSKEGKSKAMVAQYENQDRQEEVLKELFRTYVKAGVIDLDESEFDS
                     FIAYLNMPESYLRTASDYDLAVTIRQKYLQYRAAQQFHDQIQR"
     gene            10488..11216
                     /locus_tag="VFU15_09245"
     CDS             10488..11216
                     /locus_tag="VFU15_09245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718007.1"
                     /translation="MSRTKLFFPGIWIVLLVFYSCGSPSAPRPHKKNKTQDSAGKHRA
                     SVSQKPGLRYAVRLEKGDRTQFDDFFARDGSRIYYSTYKGRISYSDTLKSMQHFLFDL
                     KAEFMVDRIFIYQTDADHYFVVWQETDHEGVKSYMAGFREGAAKPDWKKTARFPNPAK
                     PVIDSTFAYYSMLGIVGKISVVSGKTEWEHDSLFNPYKHMYQEFSPAQVYPDKILFID
                     RPIAGRRPRPDTLILEPRTGKSIR"
     gene            11270..11806
                     /gene="pyrR"
                     /locus_tag="VFU15_09250"
     CDS             11270..11806
                     /gene="pyrR"
                     /locus_tag="VFU15_09250"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEU4718008.1"
                     /translation="MAPRLILTPQQFSITINRLCHQLIEVHGDFSESVIIGLQPRGIF
                     LARRIRKTLESILAGKKVPLGELDVTFYRDDFRKKDLVPNRTSIDFIIEDRNVILVDD
                     VLYTGRTIRAGLDAMMAFGRPRDVELLVLIDRRFSRNVPIQAKYVGRTVDTIASEKVM
                     VEWKEAGGEDKVWLQTQE"
     gene            11811..12743
                     /locus_tag="VFU15_09255"
     CDS             11811..12743
                     /locus_tag="VFU15_09255"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008242837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEU4718009.1"
                     /translation="MAKKLSVKHLLGIKQLQREDIELILSTAARFKDVINRPIKKVPS
                     LRDITVANLFFENSTRTRVSFELAERRLSADTVNFSASTSSVKKGETLIDTVNNILAM
                     KVDLVVMRHPNPGACVFLSKHIDASIINAGDGAHEHPTQALLDAFSIQEKLGAVEGKK
                     VAIIGDILHSRVALSNIFCLRKLGAEVMVCGPRTLMPKYISSLGVKVEYNIRKALEWC
                     DVVNMLRIQLERQDIKYFPTLREYSMQYGLDMEMLKSIGKEITIMHPGPINRGVEITS
                     EVADSGQSIILGQVENGVAVRMAVLYLLAGRTDD"
     gene            12787..13158
                     /locus_tag="VFU15_09260"
     CDS             12787..13158
                     /locus_tag="VFU15_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017259031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEU4718010.1"
                     /translation="MNQPFVVERSDRYSLIRLNAEKLDTMVAPTLKSELVVLNADGVR
                     NLIIDLSETRYCDSSGLSAILVANRLCKNANGVLVISGLQDPVKKLISISQLDSILKI
                     AGDLPGAIDLLVAEDVENSGD"
     gene            13175..13945
                     /locus_tag="VFU15_09265"
     CDS             13175..13945
                     /locus_tag="VFU15_09265"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEU4718011.1"
                     /translation="MKKIYLAASLVMVSLAGFAQCSINQGVFNGPTDYSIHPDTVTNI
                     PVAYVGVGYTTDLQFHVQPDTVTQLGTFNITHITIDSVSGIPANFSYMPNPSNGVFPG
                     GSYGCIGVTGNAVSGQETGGPNSDGVYPIIVYYTATVDVFSVPTDFPATKTGYKLRIV
                     DPNAVPSVNPLKFSVGQNSPNPADNHTEFQIVSPNGGQVQLTIFDILGSKVSDRSINV
                     AKGTSRFDLGTSDLAPGVYMCTFRMGSAIVTKRISVAR"
     gene            13945..14865
                     /locus_tag="VFU15_09270"
     CDS             13945..14865
                     /locus_tag="VFU15_09270"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003008218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HEU4718012.1"
                     /translation="MSFAFEVTILGCSSATPTSKRFPTAQLLNMAERFFLIDCGEGTQ
                     IQLRRYHIRFQRINHIFISHLHGDHYLGLMGLLSSFHLLGRKTKLNIYHPEGLKEIID
                     IQLNYSGTVFNYEIEWHVHPETDGIIFEDTLLTVETIKLDHRIPCRGFIFREKQRPFT
                     LDREKTDKHRVPVALFAEIKKGNDITLEDGTVIPNKELTKGRPRPRSYAFCSDTRFSA
                     SVTRAVKGVDLLYHEATFLHEMIDRAEKTFHSTAKEAGITAKEAGVKQLLIGHYSVRY
                     LDLRPLLEEAKSEFPETLLAEEGKKFSIAP"
     gene            14955..>15197
                     /locus_tag="VFU15_09275"
     CDS             14955..>15197
                     /locus_tag="VFU15_09275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718013.1"
                     /translation="MAINILLAQSNFLVREGLKSLINNENELVLSGEAVNPAELDMLL
                     ESTNPVVVIIDHLDPCFGIAAIRRIKTANEKNTILSI"
BASE COUNT         3397 a         4023 c         4053 g         3724 t
ORIGIN      
        1 ccgtttcccg atcaggctgc gcgcgatcgc aacgacatct ttgccggtgt agaatgatct
       61 tccgagcttt ttcatccggc gacttcgagc gtgtggtccc tggtaaatcg tacgcgttcc
      121 gcatcgagca actggcggac aatgaaattc agctcttcct ggctttcgtc cgggaacagg
      181 tccgggagct cgcggatact cctcggctgc tcacgcagcg catcgatgat cgccagcgcg
      241 aggcggtcac ggtctgtgcg cttgtttttc cccgatcttt ttttgcagac atcgcatttg
      301 ccgcaatccg gtgccccggt ctccccgaag taggcgagga gcgtgcgctg ccggcaggtg
      361 cgttcgtcgc tgatgaagcg tttcatcgcc gcaatgcgtt ctcccgccgt tttttttcgc
      421 tgtgcaagat gctcgggcga gatctgaagc gttttggcgt cggcccgccg cgtgatgaac
      481 gtgagcagcg gcttgtcgct ttgcggaacg taattcagca gacctttctg ctcgagctcg
      541 tgcagcatgc ggatcacttt ctcttcgctg acgccggttt tattggcgat ctctttttca
      601 cggatgcgga tgtattcgtc gaacaggccg ggatacatcc gcaggatagt tttgatgatc
      661 gtttcgtagt aagcgtttgc aacctggaaa cggtaaagcg tttcgttgtc ggaaatgaat
      721 ttcagccgcg aaggtgtgta aacggcatcg ttcaccgcca ggtaaccttc tttctcgaga
      781 attttcaggc tgttgaatac gttcagcgga tcgagcttgt acgtgtcgca gatctttttc
      841 tggtcgagca cgaacgtggc gccttcgccc gcaccgaccg gcacctggta aagattcgcg
      901 atcgcctggt aggtacgccg tatttcttcc tgtggcggaa acgacgattc gaagttgcgg
      961 tccagctcgc tgagatcatg ctggttccac agcaggatcg cgcctgagtc tttcccgtcg
     1021 cgtcctgctc ttccggcttc ctggaaatac gcttccgggc tgtcgggcag atcgacgtgc
     1081 gccacgaaac ggacatccgg tttgtcgatg cccatcccga acgcattggt ggaaaccatc
     1141 accttcgcct ggttgttcag ccactgctgc tgccgcgcct gcctttcctg cgcggcaagt
     1201 cccgcgtggt agaacgtcac agggattcca ttcttgtaca ggaaggaagc gatctcctgt
     1261 gttttccgcc ggttgcggac gtaaatgatc ccgctgccgt ccacctgttt cgacagcttc
     1321 agcaaatagg aattcttgtc ttctgttttt ttgacgatat aggcgaggtt cgtccgtgcg
     1381 aagctgttgc ggaaaacgct cttctttttg aaaagcagtt tttcctgtat gtcctccacg
     1441 actttctcgg ttgctgttgc ggtaagggca atgacgggaa cgccgggcag cagctcacgc
     1501 agctcgccga tgagaagata gggcggacgg aaatcatagc cccattgtga aatgcagtgc
     1561 gcctcgtcga cggcgatgag gttcactttc atcttcgcga tccgaacgcg tgcaagttcg
     1621 gtctgcagtc gttccggcga gaggtaaagg aatttaattt ttccgtagac gcacgtgtcg
     1681 aatgcagcat cctgctcgcg tttccccatg gcggaagtga tcgccatcgc gggaatgtta
     1741 cgtcgtttca ggttttccac ctggtcctgc atgagcgcga tgagcgggga aacaacgatg
     1801 cataatcctt ctttgcacag cgccgggacc tggaaacaga tggattttcc tccgcctgtg
     1861 gggagcagcg caagcgtgtc atttccgtcg agaacgcttt ggataatttc cgcctgcccc
     1921 ggccggaagg agtcgaatcc ccagtgttgt ttcagtatgg aaacaggttc ctgcacgcag
     1981 cgaagttaat gaattgaaaa gcggggaacg gtttgttccg cgtcactttc cgtcgccaag
     2041 aagcgtggtt tccgtatccc gcataagaac ggcgttccgg aatatttcat ccacggatgt
     2101 tccgccgttg tatgttttga tagtgcgtat cgacatgacc gcgccatgtc ccgggccgga
     2161 aaagccgaga atgctgtcgg ttgcttctac ggaaatagta tcgccttttt tcaggttatc
     2221 gtaatgatcg ctcttgccgg aattgaccct tgcataagag acatcgctga acagctcgtt
     2281 ctttttcccg tttttgaaat tgaagatggt gaaatacccg ttgtcgtcgc ccatccacgt
     2341 gatgccgcac tcggtgacaa tttccgggat gccgtcgccg gtgatgtctt ttacggcaga
     2401 tcttccaaac gaaccgctcg ttccgctgag gatgatcgcc gcgttcccgg tggagtcttt
     2461 ccgcacgaga acataaaagc acgcgcagtt accggaagat cctccgcggt aagcatcgtc
     2521 ttcgatgagc atttccgtgt ttttcagcga cgtgaaatga tcttccgcga tcagcacgtt
     2581 ggccgcaaca gaatccattc cctcgaaatt catgcccagg tccgacgatt cataaaacat
     2641 gaattgcagc gctttctccg gtttgcgcag ctcgtcataa tcccatgcgg cggcctctcc
     2701 ttcatctccg ccatccgaaa agtcgttacg cgggaccgtg ggttgtgcgc ttccagccct
     2761 tgagatgatc aaagaatcgc cgggcaggct gccggatttt ttttcctgcg gggaccggca
     2821 gcaggtgaat aaaactgcgg aaagtaaaac gagcttcctc aaatttcttt agaaggtgaa
     2881 aaccacgctg aactgccaca cttcgttctt gtctttgcac acgggaggaa gccaggccgg
     2941 catgtcgagc acgatctgct tcattttttc gacgacggaa gacggaagtc cgctttcgtc
     3001 aagcacgggc attaccggtt tcatggtcgt tgtgtcgatg gcgaacgtca cggtaaatga
     3061 ttgtccgtgg taaggcgcaa tttcattggg cgtgaaatcc gttcttatgc ggtgtgcgat
     3121 gtattcgttc ttcgccaggt tgccgccgaa ataacgcggc ccgcagatca gctgaggcac
     3181 gcgcagcgaa tccacgaaaa gctctttgtg cgtatcgaaa tccagcctgt cgaccagtgt
     3241 gccgctcgtg tccgatgaat aataattcca gacaccgacc ggcacgccgt ctttgtattg
     3301 gcccgtgacg agcgtttttc cgtgcgtgct gtagcgcagg taatttcctt ccttgtctcc
     3361 ttttttattt tgcgtgaact gctcgttcac tttcccgttg tggtaatgcg tggtgacgtg
     3421 cgtggtgtcc tgtgcgcgga gggaaaactg gagcaggaca aaaaatgcga gaagtgcggg
     3481 tgctgatttc atttttccaa agtacaacta ttccctgcaa aaaaaaggcc ggaattcccg
     3541 gccctctttt tagttatcat cttcgcacgc atcttccatg aaggaattcg tgtcttcatg
     3601 ccacgtgtaa ttcggaaaat acttcgggtt ctcccaccag aatttcgtgt gcttccggtc
     3661 gtagttgccg atctcgttca tcgtctgcga atcgtacagg cgcccgttca ccatcgtgta
     3721 tttcacatac tggctgttgt agatatcttc cagcgggtta ttatccagaa cgataagatc
     3781 cgcgagcttt cctttttcca gcgagccgat cgatttgtcg agaccgaggt accacgcgcc
     3841 gttcatggtg gcgcagcgca gcgcctgcat cggtgacatt cctccctgcg cgagcatcca
     3901 cagctcccag tgcgcgccga gtccctgcag ctggccgtgc gcgcccaggt tcacgcgtac
     3961 gcccgcatcc gcaagcttct tcacggattg cgaaacgaga atgtgcccgt tgttgtattc
     4021 ttcttccggg gccatcacgc ggtgacgcga gcgtgaatcg atcatgccgc gcgggtagaa
     4081 attcagcagg cggctgtttt cccagacgtt cgaatgctgg taccagtagc attccccgtt
     4141 catcgcgccg tacgttacga tcaatgtggg cgtatagccg gtctcgctgt tgctccacaa
     4201 tttcaccacg tcgtcataaa ccggtgcgac gggaatattg tgctcgatgc cggtgtgacc
     4261 gtcgaggatc atcgtcatgt tgtggtaaaa gaaagagcct ccttcgggca caaccatcat
     4321 tttcaggttg cgcgcggcgg taatgatctg ctggcgctgt tcgcgccgcg gctggttgta
     4381 gcttttcacg gaaaacgcgc cgcaggcctg tgtgcggtat acggcggaac gcgcgtcgtt
     4441 gtaattgttg atgacggcct tgaaatcgcc ttctgcgcca tagaggatcc agccggttga
     4501 aaagatccgc gggcccacca tgttgccggc tttcacggct tcactctgcg agaagaccat
     4561 ttccgtattc gaagaaggat catgcgtcgt cgttactccg aaagcaagat tcgcgtaata
     4621 gctccattgc ttttgcggtg aaagtccctg gcggaacgtc cccatgtgcg aatgcacatc
     4681 gacaaggccc ggcatgatgg tttttcccgt gcagtcgatc actttcgcat caccggggat
     4741 cttcacgttg cccatcggcc cgacttcttc aatgcggttt tcacgtacga gcacgcagcc
     4801 gttctcgatc acggagttgt tcgcatccat cgtgatgatg cgcgcattgg tgaacgccac
     4861 agcgcctttc ggcttgtcgc tctgcagcac gaggttgatc tttattcccg tcgtgtccat
     4921 gcccggcaat gaatccggcg cacccgggac gaagtcgaac gtgtttttga tttcacgcgt
     4981 gaaataacgt tcgcccattg tccagtggat ctttttgctg tccgcgctcc agtcaatgct
     5041 ctggccggcg tcgcgtgtaa tgcactgcat cggcacctgg ctcatgttgt tcgcgagctc
     5101 gagcgttttc cccgaaggcg ggaaggcgca tacatagact ttgaacagct cgcggaacgc
     5161 gatccattta ttgtccgggc tcggtacgat ctcccacgca taattcgacg tggcgacgga
     5221 acgcgccgtc ttcttgtcag gatcgtaggc tttcagctct ttcgaatcgc cgttgtcgac
     5281 ataatagaaa atctcttttc cgtcggcgga gaatgtcggt ttctgcgcgt tgtcgttgaa
     5341 caggtacgtg gcatttccac ccgctgcagg cactgtgtag attccggggt ttacgcaaaa
     5401 tgtgtaaccc tggtggtcgt tgcctgtttc tttccagaag gtgatcgtgt tcccgtcggg
     5461 tgaaaaagcc ggcgtgcgga aaatcccttt ttcattggtg atcttcacgg gcttcgcttt
     5521 cggtgatttc atgtcgagcg tcatcacggc gccggtctgt tcatcgttcc aggaaacgaa
     5581 cgcaagcttc gatccgtcgc ggctgaacgc gggctcgaat tcaaaatccg tttcgtcggt
     5641 gagacgttcg ggctttccgt cgggcaatgt ttttttccag agaaaacccg ccgcattgaa
     5701 cacgagcgtt ttttcgtcgg gagatgtaac ggcattgcgg atcattttcg ccgtgaactg
     5761 gtcgggcgca acatcctgct ggaaacgcag cgcattgtaa atgtggtgat gacaggtaac
     5821 ggaaaaagga atgtccgcca ctttctgcgt tgccacgtcg atatttttca gctttccttt
     5881 cgcccagatg acgacgtgct tgtcgtcggg tgtccagctg aacccggtat acacgccgaa
     5941 aatgcaccac gcttcctgct ggtccttgct caggtcgtca taaacaggcc attcttctcc
     6001 cgtcgcgaga tcacgcagga acagcacgga tttttcacgt acgcggcgca cgaaggcgag
     6061 aagcttgccg ttgtgcgaaa cctgcggacg gcaggcgccg ccgggaccac ccgttacatc
     6121 ttcaatttct cctgtcgtga aattgtaacg gcggatagcg tagatctggt tattgggatc
     6181 tttgttgtac tggaaataac cgccgggata catgtcttcg ctgtagtaaa cgtatttccc
     6241 gtcagcggaa acggaaggtt cgttcagatc ctgctgctgg tttttccgct gcgtgagctg
     6301 tacaccatcg ccgccggtga aatgatacat ccataattct cctgcgccga gcgagcgggt
     6361 ggaggtgaag tgcttgcggg cgatgatgta ttgtccgtca gccgaccaca cgccattgtt
     6421 cagcaggcgg aaattctcat tggtgacttc ctttgcgccg gaaccgtcgg cgttcatcac
     6481 ccagacgttg tcgccaccgc cttcatcgct ggtgaaacag atcttttttc cgtccggact
     6541 gtaacgcggc tggcattgcc aggcatggcc ggtttgcagc gggaccgcgg tgccgcccgt
     6601 tatcgggatg gaataaatat ccccgagcag gtcgaaggcg acggttttcc cgtcgggact
     6661 cacgtcgagg ttcatccacg tgccttcgtt gacggtgaac gtgacatcct tgtaatcgcc
     6721 gggaggatta ttgacatccc attttttcgg tttggcagga tcagctgcgg tgaaaaatac
     6781 ggccgcggct gcaacggcgg aaaggaagaa aatgctgtat ttcatagtgg aaaaggtgag
     6841 aggcaaagaa aacgaaaaag ccccgtcttt gagaagaaag acagggcttt taacgtgaaa
     6901 attatatggg tggtcagaaa cctactttcg gtgtgacctt ctgctcgata agaatgccga
     6961 ggcacgctgc gtctgcggat ttcccttttc cgggaccgct tccggggatc gtgatcgtga
     7021 tgaacatatt gcgcgtcgtc tcgttcgaga attcgaaatg cgtggctttg ttgtcggtcg
     7081 cattgtcgta cagaacttca ccggtggttg cgtcggaaag aaccatcccg atgccggttc
     7141 cgaggagctt gtccgcgcac atggagatcg aataatcgaa gccggaataa aaaacggctt
     7201 tcagcttcga cgtcgttccg cgggcgaaga gtccgctttt tgattgcgag ttgtagagga
     7261 atccgtcttc ggatgcctgg ctgcacccgg cctgcctgtg gtacgtcaca cagggagcct
     7321 gcgaaggaag cggcagcgca aggccggaca tcgcggcgag catgatataa acgggcaact
     7381 ttttcatggt cttgtttttt aaaaacaggg taatacaatt actgggccga aataaatcca
     7441 ttacggatat cagcgacctt ggatttgatc ttttcgaact ggtctttggt aggcgtgggt
     7501 ttcgatccgc cgccgagcac gtgcttgccg ttcttcatcg tcatgccgct ggccgtggtg
     7561 gaaacgggaa ccgcatcgaa cagcgctttg agctctttca gctggtcgga aacggctttc
     7621 acgtcgggat tcgattcgtg cttcgagagg taatccacga ggttgtcgag cgaacctttc
     7681 tgctgcacaa cgccgtcaac cgttgctttg tccttatcga aatccttcac catgttcgtc
     7741 atcaggtaaa gcgattccac ccagccgcct gcgagcatga gaccgaggtc atcgccacgc
     7801 tggttctggt cgaggttttc aaatgtggtg aggtaaagat cgttcgagat cgctaccagc
     7861 gaatcggcat tgccggcatt cttgctgatg cggtctttgt ctttatcgga aatatcggtc
     7921 gcgactttca gcttatcgcc catgcgcatg cacgttccga aataaggcac aaccttgttg
     7981 ctcacgctga acgtggagca gtacagcagg tcggctgaat aaacaccgag gttcagcgac
     8041 tgcgctttct ggctttcgta tttcttttcg ttctccacgg gattgagcac atccgcagac
     8101 gcgtttccgc ccgcagcggc tttcatgaag gcaaacattt cagagggaga agggacctgc
     8161 gcaaattcct ggttgccgat cgtgtccttc ttttcaacgg cggaagtggt atctcccatc
     8221 gtatccacgc cgtttttatc ggagttgtcg ttgttacagg aattcagggt cagtgcgagc
     8281 accgctaccg ggacggaaag cagagccggc caaaaacgtt ttttcataga gtgtcctgct
     8341 agggatggaa tggtttcagc aaaaataatc tttttatcgg atttgaaagg gcgcacgcaa
     8401 gccccggctt cgcggtaaaa acagaaaact ggttacaggt acaaccggga tctgaaatgg
     8461 gttaacctgc tgcagcagat cgtaacggtt tctccctgtt attgtgagac agaaatcagt
     8521 accggaatta aaagtgtatg cattctgtgt gacgaacgaa taaaaaaata aaccaggaaa
     8581 atcacttctt tcaatagtga acgagccatc aaatatgggg caggacagaa cggataaaaa
     8641 aaaagaaaca cccccgccga aagcgggggt gtcctgttca gtcggggtga gaggactcga
     8701 acctccggcc tccacgtccc gaacgtggcg cgctagccaa ctgcgctaca ccccgataat
     8761 tgcaaataaa gaaccctgtg acggggtgca aaaatagtat tttaaaaaca cctgccaagc
     8821 gaatagttaa gttgtgaaat caatcttccg gcagaaaccc gggccgggcc ggtttgcctt
     8881 ctgccacttg ccgtggggca acgttcactt ataagaaacg tcttcagggt aagccctttt
     8941 ctcagtagtt ttgtataatg agaaaagcaa tcctcgggct gagctgtttc atgatgatca
     9001 tttcgtcgct tcgttcgcag gttgtggtgc agggaatcgt tccgggcaat aacccgaaac
     9061 cggctcattt tcatccctgc gtgaatttcg gtctttacat tccctatgcc gttatcaata
     9121 accgtctcga ggtccgtggc agctacaagc cgggcatttc gctcatgacg ggttttcagc
     9181 gtcaccgctg gttcatgtgg acggcggaat attcgcgcta tttcacgcat acggcaagtc
     9241 cttcgttcga caatatcaaa gcgtggaatg cggaaatgaa cgggatattc ctgatgaatg
     9301 tcggcgaatc atacgtgatc tttcgcgcta ttttcggtgc gagtctgctc gactggaacg
     9361 gcaggttcat cggccccacg ctgaacgaca acaacaagtg gtcgatcggg atgaacattc
     9421 accagagctg gatcgccgcc aatctcggct gcggattcga atacaacatc tgccacggat
     9481 tgatcgcttc cgccgattac cgggcccggt tcgcttcgaa gaacggcgat ctcatcagca
     9541 tttccgatac tgcgtatttt atcggccttc gttatcgttt cgggaataat gagaacgatg
     9601 cggattccgg ttccggcgcc agacggaaat catcaacttc aaaacccggt cgcatataca
     9661 aatggctcag gcaccgtccg tcagcatgat gttccggcgc atcgccggca gcaaccgggg
     9721 aaagatattt ttcttcctcg cggccttcgt gctgctgggt tcgttttccc ccgcgcagac
     9781 ggcaacggtt acattttccg gtaaagtatt cgataagcgc gacagctccg ccacgcctgc
     9841 acccatcgtt gtgaacaagc gtacgtacac gggaagcacg ggaagcatgg acgggagctt
     9901 ttccattacc ggattaaaga ccgacacctt catcgtaacg gccagcggat atgagcttca
     9961 gcgtttctgt ttccgcgatt cgacggcgaa agacacgttc cgtctccgca tcgggctgac
    10021 gatcaaaagc actacgctcc aggctgtggc gatttacccg gtgaaagatc ttgacgcgat
    10081 caaaaaagaa agagaaagcc tcggcgtgca gcagacatac atgacaagcg gtatcaccga
    10141 tgccgttcaa agcccgatca cgtatatgta tgaacgtttc agcaaggaag gaaaatcgaa
    10201 agcgatggtg gcgcagtacg agaaccagga ccgccaggaa gaagtgctga aggaattgtt
    10261 ccggacgtat gtgaaagcgg gcgtgatcga cctggatgaa agcgaattcg attcctttat
    10321 cgcttacctg aacatgccgg aatcctatct ccgcacagca agcgattacg acctggcggt
    10381 taccatccgc cagaaatacc tgcagtaccg ggcggcgcag cagttccacg accagataca
    10441 gcgctgatga ttttcgccgc aaccttccgg gagctacctt tgcagctatg agccggacaa
    10501 aattgttttt tcccgggata tggatagtgc tgctggtttt ttattcctgc ggatcgcctt
    10561 cggctccccg gccgcataag aaaaacaaaa cgcaggattc ggcggggaag catcgcgcat
    10621 ccgtatcgca gaaaccgggg ctgcggtatg ccgtccgtct cgaaaaagga gacaggacgc
    10681 agttcgatga tttttttgcc cgcgacggga gcaggatcta ttattccact tacaagggga
    10741 ggatcagcta ttcggatacg ctgaaaagca tgcagcattt cctgttcgac cttaaagccg
    10801 aattcatggt cgacaggatt ttcatttacc agacggacgc ggatcattat tttgtcgtgt
    10861 ggcaggaaac ggatcatgaa ggcgtgaaga gctacatggc tggtttccgg gagggcgccg
    10921 ccaaaccgga ctggaaaaaa acagcccggt ttcccaatcc cgcgaaaccg gttatcgaca
    10981 gtacgttcgc ttattattcc atgctcggca tcgtggggaa aatatctgtt gtttcaggta
    11041 aaacagagtg ggaacacgat tcgcttttca atccgtacaa acacatgtac caggaatttt
    11101 ctcccgccca ggtgtatccc gataagattc ttttcattga ccgtcccatt gcgggaaggc
    11161 gtcccaggcc ggacacgctt atcctggagc cgcggacggg aaaaagcata cggtgatgcg
    11221 gatggaaaat cattatcccg gctgatttcc tatctttgcc ggtgccggta tggctccacg
    11281 attaatactt actccgcagc agttttccat tacgatcaat cgtctctgtc atcagttgat
    11341 cgaagttcac ggcgattttt ctgaatcggt catcattggc ctccagcccc gcggaatttt
    11401 tctcgcacgg cggatccgca aaacgctcga gtctattctc gccggaaaaa aagttccgct
    11461 cggtgaactg gacgttacct tttaccgcga tgatttccgg aaaaaagacc ttgttcccaa
    11521 ccgcaccagc atcgacttca tcatcgaaga caggaacgtg atcctcgtgg atgatgtgct
    11581 gtacacggga cgtacgatcc gtgccggact cgatgcgatg atggccttcg gacgcccgcg
    11641 cgatgtggaa ctgctcgtgc tcatcgatcg caggttcagc cggaacgttc cgatccaggc
    11701 aaaatatgtg ggacgcacag ttgacacgat cgcttccgaa aaagtcatgg tggagtggaa
    11761 ggaagcgggc ggagaagata aagtatggct gcaaacacag gaatgaacgc atggcgaaaa
    11821 aattaagcgt aaagcacctg cttggcatca agcagctcca gcgggaagac atcgaactga
    11881 tcctttccac agccgcccgt ttcaaagacg tcatcaaccg ccccattaaa aaagtccctt
    11941 ctctccgcga tatcaccgta gccaatcttt tcttcgaaaa ctcaacacgc acaagggttt
    12001 ccttcgagct cgccgaacgc aggctttccg cggataccgt aaatttttcc gcctccacat
    12061 cttccgtcaa gaaaggcgaa acgcttatcg acacggtgaa caatatcctc gcgatgaagg
    12121 tggatctcgt cgttatgcgc cacccgaatc ccggggcgtg tgttttcctt tcgaagcaca
    12181 tcgacgcatc gatcatcaat gcaggcgacg gggcgcacga gcacccgacg caggcgctgc
    12241 tggacgcttt ttcgatccag gaaaagctcg gagccgttga aggaaaaaaa gtggccatca
    12301 tcggcgacat acttcattcc cgcgtggcgc tttcgaatat cttctgcctg cggaagctgg
    12361 gagcggaagt gatggtctgc gggcctcgca cgctcatgcc gaaatacatt tcgtcacttg
    12421 gagtaaaggt tgaatataat attcgtaaag cattggaatg gtgcgatgtg gtgaatatgt
    12481 taaggattca gcttgaacgc caggacatca agtatttccc aaccctgagg gaatattcca
    12541 tgcagtacgg gctcgacatg gaaatgctga aaagcatcgg gaaagaaata accatcatgc
    12601 atcccgggcc gatcaaccgc ggcgtggaga tcacgagcga agtggcggat tcgggccagt
    12661 cgatcattct cgggcaggtg gagaacggcg tcgcggtgcg gatggcggtt ctttatcttc
    12721 tggccggcag gaccgacgat tgaccggcgt tgcggttcag gtttaaaaag tatatttttg
    12781 caggatatga atcagccatt tgtagttgaa agaagcgacc gctattccct gatccgcctg
    12841 aacgcggaaa agctggatac gatggtagcg cccacgctga agtcggagct tgttgtgctg
    12901 aatgccgacg gagtgagaaa cctgatcatt gatctgtccg aaacacgtta ctgcgattcc
    12961 tcgggactca gcgcgattct tgtcgccaac cgtctctgca agaatgccaa tggagtgctc
    13021 gtcatttcgg gattgcagga tcccgtcaaa aaactgatct ccatttcgca gctcgacagc
    13081 atacttaaaa ttgccggcga tcttcccggc gcgatcgatc ttctcgttgc cgaagacgtt
    13141 gaaaattccg gcgactgacc ctaaatcttt tcagatgaaa aaaatctacc tcgctgcttc
    13201 gcttgttatg gtttcgcttg ccggtttcgc gcaatgctcc atcaaccaag gtgtttttaa
    13261 cggtcctacc gattacagca ttcacccgga tacagtaacg aacatcccgg tggcgtatgt
    13321 cggtgtcggc tatacgacag acctgcagtt ccatgtccag ccggacacgg tgacacagct
    13381 cggcacgttc aacatcacgc atattacgat cgacagcgta agcggaattc ccgcgaactt
    13441 ctcgtacatg cccaatcctt ccaacggcgt tttccccggc ggaagctacg gctgcatcgg
    13501 tgtgaccgga aacgcagtga gcgggcagga aacaggcggt ccgaacagcg acggtgttta
    13561 cccgatcatt gtttattaca cagcgaccgt tgacgtattc agcgtgccga ccgattttcc
    13621 cgcgacgaaa accggctaca agctgcgcat tgtcgatccc aatgccgtgc cttccgtaaa
    13681 cccgcttaaa ttttccgtgg gacagaactc acccaatcct gccgataacc acaccgaatt
    13741 ccagatcgta agtcctaacg gcggccaggt gcagctgacg attttcgata ttctcggatc
    13801 caaagtgagc gatcgcagca ttaacgttgc aaaaggaaca agccggttcg acctcggtac
    13861 gtccgacctt gctccgggtg tatacatgtg cacgttccgc atgggaagcg ccatcgtgac
    13921 gaaaagaatt tccgtggccc gctgatgtcc ttcgcatttg aagtaacgat actcggctgc
    13981 agttccgcca ctccaacctc gaaaagattt ccgaccgctc agctgctgaa tatggctgag
    14041 cgtttttttt tgatcgattg cggggaagga acgcagatac agctgcgccg ctatcacatc
    14101 cgtttccagc ggatcaacca catttttatc agccacctgc acggcgatca ctacctgggc
    14161 ctgatgggac ttctttcgtc cttccacttg ctgggaagaa agacgaagct gaacatctat
    14221 cacccggaag ggctcaaaga gatcatcgat atacagctga attattccgg cactgttttc
    14281 aattacgaga tcgaatggca cgtgcacccg gaaacggacg ggatcatttt tgaagatacg
    14341 ctcctgaccg ttgaaacgat aaagctcgat caccgcattc cctgccgcgg ctttattttc
    14401 cgtgaaaaac aacggccctt tacgctcgac cgtgaaaaaa cggataagca ccgcgtaccc
    14461 gtggccctgt tcgcagaaat aaagaaaggc aacgacatca cgctggaaga cggaaccgtc
    14521 attcccaata aagagctgac aaagggacgc ccgcgcccgc gctcctatgc attctgcagc
    14581 gatacacgtt tcagcgcatc cgtgaccagg gcggtgaagg gcgtcgatct cctgtaccat
    14641 gaggcgacgt tccttcatga aatgatcgac cgcgccgaga agacgttcca ttctaccgcg
    14701 aaagaagcgg gcataaccgc gaaagaagca ggcgtgaagc agctgctcat cggccattat
    14761 tccgtacgct atctcgatct gcgtcctttg cttgaagagg cgaaatcgga atttcccgaa
    14821 acgctcctgg ctgaagaggg aaaaaagttt tccatcgctc cctgagtttg tcaacatttc
    14881 ccgcccgatc atttgccggt ttgaactgaa aagttacctt tgacccatct tattttgaat
    14941 tagtctaaat aaaaatggcg ataaatatcc tgcttgccca gtccaatttc cttgttcgcg
    15001 aaggacttaa atcgttgata aacaatgaaa atgagctcgt tctttcggga gaggccgtaa
    15061 atcccgcaga gctggacatg ctcctggaaa gcacgaatcc tgtggttgtg atcattgacc
    15121 atctggatcc ctgtttcggg atagccgcga tccggaggat aaaaacagcg aacgaaaaga
    15181 atacgatcct ttcgatc
//
LOCUS       DASTSF010000323         2455 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_130728,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000323 DASTSF010000000
VERSION     DASTSF010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2455
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130728"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(39..1538)
                     /locus_tag="VFU15_09280"
     CDS             complement(39..1538)
                     /locus_tag="VFU15_09280"
                     /inference="COORDINATES: protein motif:HMM:NF040439.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HEU4718014.1"
                     /translation="MKKLLILSALLISGSLLHAREITANLTWCAFNTADNHPYVETYM
                     SVVGNSVVYQKNTNGKYQASVDVFLTFTQDDSIKSVKRYILNSPELEDSSKAVTFLDL
                     QRIPLPLGLYTLQITLTDLGRQPEKPVSNWKKVIVDMDPDSVCVSHLEMLESCTPTVT
                     QNTLSKSGYDMVPYVSSFFPENMNELKFYGEVYNTTKVIHEDKFLVLYYIESAATMTK
                     MNDYSSFLKLAPEKINSFIYSFNLTKLPSGSYNLVVEVRNAFNRLLSQRKSPFERFNP
                     GITYQLTDLGAVDISNTFAGRITNIDTLTDYIRSLRPISSQPEIEFAENRIKAGDLKL
                     MQQYFYNFWLARNETNPEGAWLKYNIEVQKVNHDFSTPILRGYETDRGRVYLQYGPPD
                     QRVVETNEPSSYPYEIWQYYTIRSAQTTDGVNQPDNTVQTNKKFVFADFDLVTNNYQL
                     IHSDARGEVRDDNWQLRLVKRDNAPGNVDDQQGTPQYGGNSNDWYKNPH"
     gene            2041..>2455
                     /locus_tag="VFU15_09285"
     CDS             2041..>2455
                     /locus_tag="VFU15_09285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718015.1"
                     /translation="MNTYSLFTVIHASLASLAMLAGIIAIGAVPKGSKLHKAAGRYYL
                     YIYGAAILTGLAMLALKFNAFTLAFTVLNIYFLYMGYSFSKGNQRSNRVLNWVFLSVL
                     FLVFIQMVLSFILILGNLFTPDYGWPVVRLFYVVFT"
BASE COUNT          550 a          578 c          660 g          667 t
ORIGIN      
        1 gaagaggatg tttttggggt tcatgcgaat gaaaattatc agtgtggatt tttgtaccag
       61 tcgttcgaat ttccgccgta ctgcggtgtt ccctgctggt catcgacgtt gccgggagca
      121 ttgtcgcgct tgacaaggcg aagctgccag ttgtcgtcgc gcacttcacc gcgcgcgtcc
      181 gaatgaatga gctggtaatt gttcgtcacc aggtcgaaat ccgcgaagac gaatttcttg
      241 ttcgtctgca ccgtgttgtc cggctggttg acgccgtctg tcgtctgggc gctgcgaatg
      301 gtgtaatact gccagatctc gtacgggtag gagctgggtt cgttggtttc caccacgcgc
      361 tggtcgggcg gaccgtactg caggtaaacc cggccgcggt cggtttcata gccgcgcagg
      421 atgggcgtgc tgaaatcgtg attcactttc tgcacctcga tgttgtattt cagccacgcg
      481 ccctcgggat tcgtttcgtt gcgcgcgagc cagaaattat aaaaatactg ctgcatcagc
      541 ttcaggtcgc cggccttgat ccggttctcc gcaaattcga tctccggctg tgagctgatc
      601 gggcgcagcg agcggatgta atccgtcagc gtatcgatgt tcgtgatcct acctgcgaat
      661 gtgttgctga tgtccaccgc tccgagatcc gtcagctggt aggtgatccc gggattgaag
      721 cgttcgaagg gcgattttcg ctgggaaagc agccggttga acgcattccg cacttcaacg
      781 acgaggttgt aggaacccga tggaagcttc gtcaggttaa acgagtagat gaacgaattt
      841 atcttttccg gggccagctt caggaacgag gagtaatcgt tcattttggt catcgtagca
      901 gcagattcga tgtaatagag cacaaggaat ttgtcttcat gaatgacttt tgtggtgttg
      961 tacacttccc cgtaaaattt cagctcgttc atgttttccg ggaagaacga ggaaacgtaa
     1021 ggcaccatgt cgtagccgct tttggaaagc gtgttctgtg tgacagtggg ggtgcacgat
     1081 tccagcattt cgagatgcga aacgcacacg gaatccgggt ccatgtcgac gatcactttt
     1141 ttccagttcg acaccggttt ttccggttgc cgcccgagat cggtgagcgt gatctgcagc
     1201 gtatacaaac cgagcggaag cggaatgcgt tgcaggtcga ggaacgttac cgccttgctg
     1261 gagtcttcaa gctctgggct gttgaggata taacgtttca ccgacttgat ggagtcgtcc
     1321 tgcgtgaagg tgaggaaaac atcgaccgaa gcctggtatt ttccattggt gtttttctgg
     1381 taaaccaccg aattccccac gaccgacatg taggtttcca cgtacggatg attgtcagcc
     1441 gtgttgaatg cgcaccacgt gaggttggcg gtaatttccc gggcgtgcag caaagagccc
     1501 gatatcagaa gcgcggaaag aatcagaagc tttttcatga tgaacctgtt tgacaggccg
     1561 gagtacgagg gggatggaca cattgtcccg gcaaaattat acactcaaag ttacgaaaaa
     1621 ccgcagcata acggtaaggc cggaaccgga acctgacagg cggttgcctg caatgcgtaa
     1681 gtggaaggcg ggaagcgcgt gatgcggggg aggaggaaaa ataatgccct ctatttccgg
     1741 gacaggaatg gaggcaaacg gtttttgttt tttgcctcgc gaatacaaac aagtttgatt
     1801 cgctcaccct aaaaacaaaa ccccgcagtg cggggccttt gtttcattgg cagagagaga
     1861 gggattccct gcatgatccc atttctccca ttgtcagaat cagatgaagc gctgcgctat
     1921 ttaaaatttt tccgcgaatc tgcgacagcc gtgttctaag gccattacgt ttttttaatg
     1981 agtatctttc cccatacata gaaatttata tttttaaact ctctctaatc ccgatcataa
     2041 atgaatacat actccttgtt cacagttata cacgcctcac tggcgtccct ggctatgctt
     2101 gccgggatca ttgcaattgg agcagttccc aaagggagca aactacataa ggctgccggc
     2161 aggtattatc tctatatcta tggggctgcc atcctaaccg gcctggcgat gctggctttg
     2221 aaatttaacg cgttcaccct ggcatttaca gttctcaaca tctattttct atacatgggg
     2281 tattcttttt ccaaagggaa tcaaagaagc aaccgcgtat tgaattgggt tttcctttcg
     2341 gtcttgttcc tggtttttat tcaaatggtt ttgtccttca tactcatatt gggcaatctt
     2401 ttcactcccg attatggctg gccggtggtc cggttatttt atgtcgtatt cacgc
//
LOCUS       DASTSF010000324         2557 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1146216,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000324 DASTSF010000000
VERSION     DASTSF010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2557
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1146216"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..851
                     /locus_tag="VFU15_09290"
     CDS             <1..851
                     /locus_tag="VFU15_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014682517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEU4718016.1"
                     /translation="CNAISYIGADHLFIDVDRDTMGLSPAKMEEYLSANAEIRDDGAY
                     LKKTNRRIAACVPMHSFGQALRIKELTQLCERYKIPLVEDAAESLGTTVDGKHTGTFG
                     LLGVYSFNGNKTVTCGGGGAIVTDNEALAKKAKHLTTQAKVPHRWEFVHDAIGYNYRL
                     PNINAALACAQMEQLEVFIADKRETTAAYREFFTKMGVPFVSELPGVRSNCWLFPLLL
                     NDRKERDEFLTYTNDNGVMTRPAWKLMNQLEMFKDAMKGDLGNAEWLDDRLVNIPSSA
                     RMKKTK"
     gene            848..1852
                     /gene="neuB"
                     /locus_tag="VFU15_09295"
     CDS             848..1852
                     /gene="neuB"
                     /locus_tag="VFU15_09295"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004490142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HEU4718017.1"
                     /translation="MKHVIIIAEAGVNHNGDMELAKKLVDAAAEAGADYVKFQTFKTE
                     LLVSPGAKKAEYQVKNTGENDSQFAMLKKLELGEEAHEMLAASCAKKKIRFLSTAFDH
                     ESVELLKKFDPDFFKIPSGEITNLPYLEMIAAAGKKVILSTGMSTMDEIGEALDVLRK
                     GGVRIDDITVLHCNTEYPTPMEDVNLNAMISIREKFGVAAGYSDHTEGIEVPVAAVAL
                     GATIIEKHLTLDKTLPGPDHKASLDPAEFSAMVKAIRNIEKAMGDGVKKPSPSEMKNI
                     SVARKSIVAARDIEAGEIFTSENLAVRRPGDGLSPMKWKSVIGKKAGRKFLKNEKIAL
                     "
     gene            1849..>2557
                     /locus_tag="VFU15_09300"
     CDS             1849..>2557
                     /locus_tag="VFU15_09300"
                     /inference="COORDINATES: protein motif:HMM:NF014686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEU4718018.1"
                     /translation="MKKQVTIVRSAVSSLPSAGIINLLRENGFRIAGMDLTDKSAGRH
                     LVDSFHLVSRSSESTRDKVIREIIAIAGMEKADWIISGPENEIALLASAEDQFTAMGC
                     FLFHPVAETLRIVTDKAKLNIFLSENNFPVMPFAYKGSVIPGFGPGELVFKPSKGRGS
                     SGVKFIRNTKEEISAVLSEAGEEEFILQPRLKGKEYTVDVLCDMSGRVTDIVPRERIA
                     VESGISVASKTVKNESLT"
BASE COUNT          673 a          621 c          748 g          515 t
ORIGIN      
        1 cgtgcaacgc catttcctat atcggcgcgg atcatctttt cattgatgtc gaccgcgaca
       61 cgatgggatt gtcgccggcg aagatggaag aatatctttc cgcgaatgcg gagatccgcg
      121 acgacggcgc ttacctgaaa aaaacgaacc ggcgcatcgc ggcctgcgtt cccatgcaca
      181 gcttcgggca ggcgctgcgg ataaaagagc tcacgcagct gtgcgaacgg tataaaattc
      241 cgctcgtcga agacgctgcg gaatcgctcg gcacgacggt ggacggaaaa cacaccggta
      301 catttggcct gctcggcgta tacagcttca acgggaacaa gacggtcacg tgcggcggcg
      361 gcggcgccat cgttacggat aatgaagcgc tcgcgaaaaa agcgaaacac ctcaccacgc
      421 aggcgaaagt tccgcaccgc tgggaattcg tgcatgacgc aataggttac aattaccgtt
      481 tgccgaatat caacgcggct cttgcctgcg cgcagatgga acagcttgaa gtattcatcg
      541 ccgacaagcg tgaaacgacg gctgcttacc gggaattttt tacgaaaatg ggcgtgccgt
      601 tcgtaagcga gctgcccggc gtccgttcca attgctggct gttcccgctt ttgctgaatg
      661 accggaaaga gcgcgatgag tttctcacct atacgaacga taatggcgtg atgacgcgtc
      721 ccgcctggaa gctgatgaac cagctggaaa tgttcaaaga cgcgatgaag ggagatctcg
      781 gtaacgcgga atggctcgac gacaggctgg tgaacattcc cagctcggcg cgaatgaaaa
      841 aaacgaaatg aagcacgtga tcatcatagc ggaggcgggc gtcaatcaca acggtgatat
      901 ggagctggcg aaaaagctcg tcgatgcggc ggcggaggca ggtgcggatt acgtgaagtt
      961 ccagacgttc aaaacggaac tgctggtaag tcccggtgca aaaaaagcgg aataccaggt
     1021 gaagaatacg ggtgaaaatg attcccagtt cgcgatgctg aaaaagctcg agctcggcga
     1081 agaagcgcat gaaatgcttg cggcttcttg cgcgaaaaag aagatcaggt tcctgtccac
     1141 tgcgttcgac catgaaagcg tggagctgct gaaaaagttc gaccccgatt ttttcaaaat
     1201 accatccggc gaaataacga accttccgta cctcgaaatg atcgccgcgg cagggaaaaa
     1261 agtcatcctt tccacgggca tgagcacgat ggacgaaatt ggtgaagcgc ttgacgtgct
     1321 gcggaaaggc ggcgtgcgca ttgacgacat aaccgtgctg cattgcaaca cggaatatcc
     1381 cacgccgatg gaagacgtga acctgaacgc gatgatcagc atccgggaaa agttcggtgt
     1441 ggcagcgggc tattctgatc acacggaagg gatcgaggtc ccggtggctg cggtggcgct
     1501 cggtgcgacg atcatcgaga agcaccttac gctcgataaa acgcttcccg gcccggatca
     1561 caaggcgtcg ctcgatccgg cggaattttc cgcaatggtg aaggcgatcc gcaacatcga
     1621 aaaagcgatg ggcgacggtg tgaagaaacc ttcgccttcc gaaatgaaaa atatttccgt
     1681 tgcgcgcaaa agcattgtcg ccgcgcgcga cattgaagcc ggtgaaattt tcacaagtga
     1741 aaatcttgcg gtcaggcgac ccggcgacgg gctgagcccg atgaaatgga aaagcgtgat
     1801 cgggaagaag gcggggagga aattcctgaa aaacgagaag atcgccctat gaaaaaacag
     1861 gtgaccattg tccgttccgc ggtttcttca ttgccttccg ccggtatcat caacctgctc
     1921 agggagaatg gattcaggat agccggcatg gatcttacgg ataaaagtgc cgggcgtcac
     1981 ctcgtggatt cgttccacct ggtttcgcga tcatcagaaa gtacgcgcga caaagtgatc
     2041 cgggaaatta ttgccattgc cggtatggaa aaagcggact ggatcatttc cggtcctgaa
     2101 aatgagatag cgcttctcgc ctccgcggaa gatcaattca cggcgatggg atgctttctc
     2161 tttcacccgg tcgctgaaac gctcaggata gtcacagata aagcgaagct gaatatattt
     2221 ctttctgaaa ataatttccc ggtgatgcct ttcgcataca agggttctgt cattcctgga
     2281 ttcgggcctg gagaattggt tttcaaaccg tcgaaaggca ggggaagcag cggggtgaaa
     2341 tttatccgga atacgaaaga agaaatttcg gctgttcttt cggaagccgg tgaagaggag
     2401 ttcattcttc agcccaggct gaaaggaaaa gaatacacgg ttgacgtgct ttgcgacatg
     2461 agcggccggg tgacggacat cgtgccgcgg gaaaggattg cggtcgagtc gggaatttcc
     2521 gtggcgtcaa agacagtgaa aaacgaatcg cttacag
//
LOCUS       DASTSF010000325         7338 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1598532,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000325 DASTSF010000000
VERSION     DASTSF010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 7338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7338
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1598532"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="VFU15_09305"
     CDS             complement(<1..242)
                     /locus_tag="VFU15_09305"
                     /inference="COORDINATES: protein motif:HMM:NF018770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1365 family protein"
                     /protein_id="HEU4718019.1"
                     /translation="MHNRLGPKKHRFHYSVFMFCLDLDALDEDLREISLVKRNRFSLF
                     SFYDKDHVVNEQGTQNAGNGVRSELDFFLRKNGMEKP"
     gene            complement(315..1574)
                     /locus_tag="VFU15_09310"
     CDS             complement(315..1574)
                     /locus_tag="VFU15_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010568254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU4718020.1"
                     /translation="MQKLAIIGTGIAGMSCGYFLRNDFELAIYEKNDYAGGHTNTIAA
                     DEDEGKVFFDTGFMVFNHVTYPLLTKLFGELKVETKKTSMSFSVQHVPSGLEYCGSGL
                     NGLFAQRKNLFNFRFIRMLKEISRFNSASVKWMNGAPDENLTIAQFCAELGLGNDFLH
                     RYLVPMSGAVWSTPPEKMEEFPALTLVRFFYNHGFLGLNTQHQWYTVEGGSRMYRDKL
                     IAPYKEKIMTGNAAVSVKREGGKVKVVSADGAERMFDKVIFASHGDETLRMLADATGK
                     ERALLSCFRYEANKATVHTDGSVMPRTKRAWSSWNYRITQEDGKEIPSTIYWMNSLQG
                     VSGKKDYFVSINGAGEIDRSKVIAEIDYTHPLFDVPAMRAQKELPLLNESGPVYFCGS
                     YFRYGFHEDALLSSVMLCEKLTGRKVL"
     gene            complement(1784..2236)
                     /gene="dtd"
                     /locus_tag="VFU15_09315"
     CDS             complement(1784..2236)
                     /gene="dtd"
                     /locus_tag="VFU15_09315"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016650218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HEU4718021.1"
                     /translation="MRVLIQRAAKASVTIDGEVRSSIGNGLLVLLGIEDADTAEDIRW
                     LTQKIVNLRIFGDAEGVMNLAVKEVNGDILVVSQFTLHASTKKGNRPSYIRAAKPPVA
                     IPLYENFVKQLETDLGKKVMTGEFGADMKVELVNDGPVTIWMDSKNRE"
     gene            complement(2287..3234)
                     /gene="rsgA"
                     /locus_tag="VFU15_09320"
     CDS             complement(2287..3234)
                     /gene="rsgA"
                     /locus_tag="VFU15_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015812656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEU4718022.1"
                     /translation="MKNEKGLVIRSTGSWYTVRMKDGTNTECRMMGQFRVKGVNTKST
                     NPITVGDHVEVEMDGAEGTGLIVGLDDRKNYIIRRSTKMSKQTHIIAANIDHAYVMAT
                     LIRPRTSTGFIDRFLVTAEAYGIPASVIFNKRDLLDEELEDYVLALMELYTGIGYPCY
                     FLSATHDADVEELRKVLHGKVNLFAGHSGVGKSTLINALEPSLDLRTGEISKAHNKGM
                     HTTTFAEMHELQGGGWIIDTPGIKELGIVNMEKEELSHYFPEMRALMGKCKYNGCLHD
                     KEPGCAVKLAVENGEIAFTRYECYLQILNGEELEKEYDD"
     gene            3378..4286
                     /gene="gldA"
                     /locus_tag="VFU15_09325"
     CDS             3378..4286
                     /gene="gldA"
                     /locus_tag="VFU15_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014203150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     ATP-binding subunit GldA"
                     /protein_id="HEU4718023.1"
                     /translation="MSIQVEYISKVYGEQRALDNVSFEVKSGEIVGFLGPNGAGKSTM
                     MKILTCFIPPTAGTAKVNGYDIMNNSIEVRRSVGYLPEHNPLYHDMYVREYLEFVAGL
                     HQLRNVRERVKEMISITGLEVEQHKQIGALSKGYRQRVGLAQALIHDPKVLILDEPTT
                     GLDPNQLAEIRELIRKIGKEKTVMLSTHIMQEVEILCDRVIIINNGKIKTDKNTATLM
                     QTDENRIVVIVEFDKAPDRHALKRVNGVEDLKAVSETTWHIIAKPDTDIRPDLFRFAV
                     DNKIMVLKMVQEEKKLEDIFKELTKK"
     gene            complement(4954..5763)
                     /locus_tag="VFU15_09330"
     CDS             complement(4954..5763)
                     /locus_tag="VFU15_09330"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEU4718024.1"
                     /translation="MKKYLFVLFLFLVPLCLLAGKDVPWKEGKFRTASSGLQYRIVKA
                     GKGDTICGTDRVTVDFVWYRRSDKSVIYNSKKDYPGGQEVTMNDPNIVKGFAIAIKML
                     KKGGKGYFKVPPSLGFGKKMKAGEDTLLYYITVKKIVHVPGVSPIINTSSDTIVFHIP
                     DPADQYRGDSLFTLAKLVDVPSIVPCGMKLVMVAFRFQLTWFDNGVQRKDILVYVQCP
                     ENYGKDYFVKGQNYVLTAIPLSENLKEGKTVYNAYSGEKLDAYYGLRVVKK"
     gene            5902..7164
                     /gene="metK"
                     /locus_tag="VFU15_09335"
     CDS             5902..7164
                     /gene="metK"
                     /locus_tag="VFU15_09335"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEU4718025.1"
                     /translation="MSYLFTSESVSEGHPDKVADQISDALIDHFLAYDANSKVACETL
                     VTTGQVVLAGEVKSKAYLDVQDIARDVIRKIGYTKAEYMFEANSCGVLSAIHEQSPDI
                     NQGVERKKPEEQGAGDQGMMFGYANRDTDNFMPLPLELSHRLLEELAAIRREGRQMKY
                     LRPDSKSQVTIEYSDHHKPIRIDTIVISTQHDDFASEKQMQQQIRKDVIGVLVPRVMK
                     KLPKRVRDLFNDKITYHVNPTGKFVIGGPHGDTGLTGRKIIVDTYGGKGAHGGGAFSG
                     KDPSKVDRSAAYATRHIAKHLVASGVCDEALVQVAYAIGVAQPVGLYVNTYGTSKVDL
                     SDGEIAEKIAGIKAFDMRPYFIEKRFNLRTPIYSETASYGHMGRESKTVTKIFNKGKE
                     NEKHVKVKLFPWEELNAIADMKKAFGLK"
BASE COUNT         1666 a         2198 c         1731 g         1743 t
ORIGIN      
        1 ggtttttcca tcccgttctt ccgcaggaaa aaatccagct cgcttctcac gccgttaccg
       61 gcattttgcg tcccctgctc attcaccacg tggtctttgt cgtaaaagga aaaaagcgag
      121 aaccggttgc gcttcaccag cgaaatttcc cgcagatcct catccagcgc atccaggtcc
      181 aggcagaaca tgaacacgct gtaatggaaa cggtgcttct tcggccccag ccggttatgc
      241 attacgttcg ccctgtacaa aaatgacctc atagccaatc cttcaatccg ttcatctttt
      301 catttcaaaa tctttcaaag caccttcctt cccgtcagct tttcgcacaa catcaccgac
      361 gacaacagcg catcttcatg gaagccgtaa cggaaataac ttccgcagaa atacaccggc
      421 ccgctttcat tcagcaacgg caactccttc tgcgcacgca tcgccggcac atcgaacagc
      481 gggtgcgtgt aatctatttc cgcgatcact ttcgagcgat cgatctcacc ggccccgttg
      541 atggaaacga aataatcctt tttccccgaa acgccctgca ggctgttcat ccagtaaatg
      601 gtcgatggaa tttctttccc gtcctcctgc gtaatgcggt agttccacga cgaccacgca
      661 cgtttcgtgc gcggcatcac cgatccgtcc gtgtgcaccg tagctttgtt cgcttcgtac
      721 cggaaacacg acagcagcgc cctctccttc ccggtcgcgt ccgccagcat gcgcagggtt
      781 tcatcgccgt ggctggcaaa gatcacttta tcgaacatgc gttctgcgcc gtccgccgaa
      841 accactttca cttttccgcc ttcgcgtttt accgacaccg cggcatttcc tgtcatgatc
      901 ttttctttgt acggcgcgat cagcttatcg cggtacatgc ggctgccgcc ttccaccgtg
      961 taccactggt gctgcgtgtt cagcccgaga aagccgtggt tgtaaaaaaa acgcacgagc
     1021 gtcagcgcgg gaaactcttc catcttttcc ggcggcgtcg accacacggc cccgctcatc
     1081 ggcacaaggt aacggtgcag gaaatcgttc cccaaaccca gctccgcgca aaactgcgcg
     1141 atcgtcaggt tttcatccgg cgcaccgttc atccatttca ccgatgccga attgaagcga
     1201 gaaatttcct tcagcatgcg gatgaagcgg aaattgaaaa ggttcttccg ctgcgcaaaa
     1261 agtccgttca atcccgaccc gcagtattcc agtcccgaag gcacatgctg cacgctgaac
     1321 gacatgctcg tctttttcgt ctccaccttc agctccccga aaagtttcgt cagcagcggg
     1381 tacgtcacgt ggttgaacac catgaaaccc gtatcgaaaa aaactttccc ctcgtcttcg
     1441 tccgctgcaa tggtattcgt gtggccacct gcataatcgt tcttctcgta aatcgccagc
     1501 tcgaaatcat tccgcaggaa atacccgcag ctcatgcccg ctatgcccgt tcctatgatg
     1561 gccagtttct gcaaactaat ttctgttcgg gcgtgcccgc ctggaagaag tccttccggc
     1621 ggtcgtgcta tccgcttgta ctcctcgccc gccgcggaac ttcgtccgcc cgggctgcgg
     1681 ggtaccgctt ctatcactca cgcgtaaatg gtttcccgca atatacggca gattgagcag
     1741 gccggttttc caaaagtatt tcctgaagtg ctgagccgga agatcactcc ctgtttttcg
     1801 aatccatcca tatcgtcacc ggtccgtcgt tcaccagctc cactttcatg tccgcgccga
     1861 attcacctgt catcactttt ttgccgagat ccgtttccag ctgtttcaca aaattttcat
     1921 acagcgggat cgctaccggc ggcttcgccg ccctgatgta cgaaggccgg ttcccctttt
     1981 tcgtcgacgc atgcagcgtg aactggctca ccacgagaat atcgccgttc acttccttca
     2041 ccgccaggtt catcacacct tccgcatcac cgaatatgcg caggttcaca atcttctgcg
     2101 tcagccaccg gatgtcttcc gccgtgtccg catcttctat tcccagcagc accagcagcc
     2161 cgttcccgat ggacgatctc acttctccgt caatggtcac ggaagctttc gctgcgcgtt
     2221 gaatgagcac tctcataact cagtttttca atggcaattc cgggttcccg ctttcacgct
     2281 tcatcgttaa tcatcatatt ccttctccag ctcttccccg ttcaggatct gcaggtagca
     2341 ttcataccgc gtaaacgcga tctcgccgtt ttccactgcc agcttcaccg cgcagccggg
     2401 ctctttgtcg tgcaggcagc cgttgtattt gcatttcccc atgagcgcgc gcatctcggg
     2461 aaaataatgc gacagctctt ccttttccat gttcacgata cccagctcct tgatgcccgg
     2521 tgtgtcgatg atccagccgc cgccctgcag ctcgtgcatt tcagcgaacg tcgtggtgtg
     2581 catgcctttg ttgtgcgcct ttgaaatttc gcccgtgcgc aaatccagtg aaggctccag
     2641 cgcattgatc agcgtgcttt ttcccacgcc ggaatgtccc gcaaaaaggt tcactttccc
     2701 gtgcagcacc ttgcgcaatt cttccacgtc tgcgtcgtgc gtggccgaaa gaaaatagca
     2761 cgggtagccg atgccggtgt acagttccat cagcgccagc acgtaatctt ccagctcttc
     2821 gtcgagcagg tcgcgcttgt tgaagatcac cgaagcggga attccgtatg cttccgccgt
     2881 aaccagaaag cggtcgataa agccggtgga tgtgcgcggg cggataagcg tagccatcac
     2941 gtaggcgtga tcgatgttcg ccgcgatgat gtgcgtctgc ttcgacattt tcgtcgacct
     3001 gcggatgatg taattcttcc ggtcgtccag tcccacgatc agccccgtgc cttccgcccc
     3061 gtccatttcc acttccacat ggtcgcccac ggtgatcggg ttcgtgcttt tcgtattcac
     3121 tcccttcacg cggaactgcc ccatcatgcg gcattcggta ttcgttccgt ccttcatccg
     3181 cacggtgtac cagcttccgg tggaccgtat cacaagccct ttttcgtttt tcacgttcca
     3241 aatgtacaaa cgccgcggaa agatgctccc gtctccccgt tttcattgat cattcctcct
     3301 ttccccgata acacacgtta acaaacaact ccgtcccttt tgcgtacttt cgtgagtatc
     3361 acaaacgttt tctatacgtg tccatccagg tcgaatacat ctcgaaagtc tatggcgagc
     3421 agcgcgccct cgacaacgtt tccttcgaag taaaatcagg cgagatcgtc ggtttcctcg
     3481 gtcccaacgg agccggcaaa tccacgatga tgaagatcct cacgtgcttc attccgccca
     3541 cggcgggaac ggcgaaggtg aacggttacg acatcatgaa caattccatc gaggtgcggc
     3601 gcagcgtggg ctacctgccg gagcacaacc cgctgtacca cgacatgtac gtgcgcgaat
     3661 acctcgagtt cgtggccggt cttcaccagc tgcgcaacgt gcgggaacgc gtgaaggaaa
     3721 tgatctccat caccgggctg gaagtggagc agcacaagca gatcggcgcg ctttcgaaag
     3781 gttaccgcca gcgtgtgggg cttgcgcagg cgctcatcca cgatccgaag gtgctcatcc
     3841 tcgacgagcc gaccaccggt ctcgatccga accagctggc cgagatccgc gaactcatcc
     3901 gcaagatcgg gaaggaaaag accgtcatgc tttccacgca catcatgcag gaagtggaaa
     3961 ttctctgcga ccgcgtgatc atcatcaaca acggaaaaat aaaaaccgac aagaatacgg
     4021 ccacgctcat gcagaccgac gagaacagga tcgtcgtgat cgtggagttc gacaaggcgc
     4081 ccgaccgtca cgcgctgaaa cgcgtaaacg gcgtggaaga cctcaaagcc gtttcagaaa
     4141 ccacgtggca catcattgcc aaacccgata cggatatccg ccccgacctt ttccgcttcg
     4201 ccgtcgacaa taagatcatg gtgctgaaaa tggtgcagga agaaaagaag ctcgaggata
     4261 ttttcaagga gctcacgaaa aaataaccgg gcggttttct gtttcggatt ttacgtttag
     4321 cgttactggc tcacttcgtc cggatcttct ttgtgctcct ttgcgtttga aaaaaaccgc
     4381 cccgcgtttg ggatccggga tttgaatttt ccggtttatt attctctgtg gcgctccgtg
     4441 aagccacctc ggtggttctc tgtgctgaaa aagaattttc ctcaaagccg ttaaaatcaa
     4501 aagaaaaaat cagtccgcgc gaagtgggaa cgtcttggca ttccggatct atccctgccc
     4561 ggatcttttt gtgaccttgg tgaaacagct tcgtgttctt cgtggttgaa aaaaaccgcc
     4621 ccgcgtttgg gatctgggat ttgaatttta cgttttgtta ttctctgtgg cgctccgtga
     4681 agccacctcg gtgattctct gtgctgaaaa agaatttccc tcaaagccgt taaagtcaaa
     4741 agaaaaaatc agcccggaca aagcgcggaa cggtttggag tttcgaattt atccctgccc
     4801 ggattttctt tgtgaccttg gtgaaacagc ttcgtgttct tcgtggttga aaaaaaatgc
     4861 cacgcgtttg ggattttgca gtttccccac atccgttttc ctctcacaac ttgttctggc
     4921 gaaactttga accgttgaat tctttccccg cagttatttc ttcaccaccc tcaaaccata
     4981 atacgcatcc agcttctcac cggaatacgc attataaacc gtcttccctt ccttcaggtt
     5041 ttccgaaagc gggatcgccg tcagcacata gttctgccct ttcacaaaat aatctttccc
     5101 gtaattttcc gggcactgca catacacaag aatatctttc cgctgcacac cgttatcgaa
     5161 ccacgtcagt tggaacctga acgccaccat caccagcttc atcccgcagg gcacgatcga
     5221 aggcacatcc accagcttcg ccagcgtgaa cagcgaatcg ccgcggtact ggtcggccgg
     5281 atccggtata tgaaaaacga tcgtatcgct cgacgtattg atgatcggcg aaacacccgg
     5341 cacgtgcacg atctttttca cagtgatgta atagagcagc gtatcctccc cggccttcat
     5401 ctttttcccg aagcccagcg aaggcggcac cttgaaatat cccttcccgc ctttcttcag
     5461 catctttatc gcgatcgcaa atcctttcac gatgttcggg tcgttcatcg tcacctcctg
     5521 cccgcccggg taatcttttt tactgttgta aatgacggat ttatcggagc gcctgtacca
     5581 gacgaaatcc accgttacgc ggtccgttcc gcagatcgta tcgccttttc ccgctttcac
     5641 gatccggtat tgcagacccg atgaagccgt ccggaatttc ccctctttcc acggtacgtc
     5701 ctttcccgcc aaaaggcaca acgggacgag gaacaaaaac aggacgaaga gatatttttt
     5761 catcgcgcta atttcaggaa atacttcaaa aacggtgccc cgtttcgtca atgaaatccg
     5821 gttaaatata cgtttttagc gacctgttcc gaatcgcaaa aaaccgtact ttagcgcacc
     5881 ataaaaaaca acagcgaaac aatgtcttac cttttcactt ccgaatccgt ttccgaaggt
     5941 cacccggaca aagttgcgga ccagatctct gacgcgctca tcgatcattt ccttgcttat
     6001 gatgcgaatt ccaaagtggc ctgcgaaacg ctcgtcacca cggggcaggt cgttcttgcc
     6061 ggcgaagtaa aatcgaaagc gtacctcgac gtgcaggaca tcgcgcgcga cgtgatccgg
     6121 aagatcggct atacgaaagc ggaatacatg ttcgaagcga attcctgcgg cgtgctttct
     6181 gctatccacg aacaatcgcc cgacatcaac cagggcgttg aacgtaaaaa accggaagag
     6241 cagggcgccg gcgaccaggg catgatgttc ggctacgcca accgcgacac cgacaatttc
     6301 atgccgcttc cgctcgagct ttcccatcgc ctcctcgaag agctggccgc catccgccgc
     6361 gaaggaagac agatgaaata ccttcgcccc gattccaaaa gccaggtaac gatcgaatac
     6421 agcgatcacc acaagcccat ccgcatcgac accatcgtca tctccacgca gcacgatgat
     6481 ttcgcttctg aaaaacagat gcagcagcag atccgcaaag atgtgatcgg cgttctcgtt
     6541 ccgcgtgtca tgaaaaaact tccgaaacgc gtgcgcgacc tgttcaatga caagatcacc
     6601 taccacgtga acccgacggg caagttcgtc atcggcggac cgcacggcga cacgggcctt
     6661 acgggacgca agatcatcgt cgacacgtac ggcggcaaag gcgcgcacgg cggcggcgca
     6721 ttctcgggaa aagatccttc gaaagtcgac cgttcggcag cgtatgcgac gcgtcacatt
     6781 gcgaagcacc tcgtggcttc cggcgtatgc gatgaagcgc tcgtgcaggt ggcctacgcg
     6841 atcggcgtgg cgcaaccggt aggactctat gtgaacacgt acgggacttc caaagtcgac
     6901 ctgagcgacg gggagatcgc ggaaaaaatt gccggcataa aagcgttcga catgcgcccg
     6961 tacttcatcg aaaaacgctt caacctccgc acacccattt acagcgaaac cgcgtcctac
     7021 gggcacatgg gccgcgaaag caaaacggtg acgaaaattt tcaataaggg aaaagagaat
     7081 gaaaagcacg tgaaggtgaa acttttcccc tgggaagagc tcaatgcgat cgcggatatg
     7141 aagaaggcgt ttgggttgaa gtaggtcccg gacattagcc ggaaggcttg acaggttcag
     7201 aggcacccgg gaacgggtgc ttttttttgg ccgcgtaaga ctttccacgg aaccggttgc
     7261 gggcacggcc ttttcacctg aattttgatc aaaactccgc gcatgaaaag acgttacatt
     7321 ctcagcctcg gcttactg
//
LOCUS       DASTSF010000326         4518 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_339274,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000326 DASTSF010000000
VERSION     DASTSF010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4518
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_339274"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(124..453)
                     /locus_tag="VFU15_09340"
     CDS             complement(124..453)
                     /locus_tag="VFU15_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008867396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system Fe-S cluster assembly protein"
                     /protein_id="HEU4718026.1"
                     /translation="MPATIIAKNAELAQKIIDAIKTCYDPEIPVDIWELGLIYGIDVS
                     DDNEVKILMTLTSPNCPAAESLPPDVENKVRDVEGVRAATVEVTFDPPWEKDMMSEVA
                     QLELGFM"
     gene            complement(467..892)
                     /locus_tag="VFU15_09345"
     CDS             complement(467..892)
                     /locus_tag="VFU15_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HEU4718027.1"
                     /translation="MSIIETENEIIDEFSMFDQWDDKYQYIIDLGKSLAPMDEPLKTD
                     DRLIKGCQSRVWLHTELKDGKVIYHADSDAIITKGLVALMVRVLSSHTPKEIVDAPMD
                     FVDRIGLKDHLSPTRSNGLLSMIKQMKLDALALSVKNKS"
     gene            complement(889..1272)
                     /locus_tag="VFU15_09350"
     CDS             complement(889..1272)
                     /locus_tag="VFU15_09350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718028.1"
                     /translation="MKTVLHIFLLLASAVSCRQQVAPAGTLTGKWSHEHEEKLFDGPW
                     GNAREHADLILNADSTFSLLIEWAGSDFPGEYNGKWSLHEDRIILHDTTANFHGENDL
                     TFHVRRNGTLLWQNERDRVFEKAKK"
     gene            complement(1269..2522)
                     /locus_tag="VFU15_09355"
     CDS             complement(1269..2522)
                     /locus_tag="VFU15_09355"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011551276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEU4718029.1"
                     /translation="MTKTVSHIIPGSAYDILQLRSEFPILSRQVNGKPLVYFDNGATT
                     QKPQAVLSCITDYYSNYNANIHRGVHHLSQEASQAYENARATVQAHLRANSPDEIIFT
                     RGTTESINLVAQSYLRNRLQPGDEVLVTEMEHHSNILPWQLLCEERGAALRVLPVNDD
                     GELEIALLQKLFSPKVKFFAFAHVSNTLGTVNPAKELTELAHAHNIPVLIDGAQAVPH
                     MAVNVNETGCEFYCFSGHKVYGPTGIGALYVRDGILDEMMPYQVGGGTIKTVTFEKTE
                     YISGPLRFEAGTPNIEGAIALAAALDYVDAIGMANIAAHEHELLVYATEKLNAIPGMR
                     IIGTAKEKAGVISFVVGNHHPFDVGTILDQQGIAVRTGHHCTQPLMQRFGIPGTVRVS
                     FALYNTKEEIDSLITALMKAVKMLS"
     gene            complement(2525..3826)
                     /gene="sufD"
                     /locus_tag="VFU15_09360"
     CDS             complement(2525..3826)
                     /gene="sufD"
                     /locus_tag="VFU15_09360"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEU4718030.1"
                     /translation="MSTVAEKNPVAEKLMEGFPGKLPSLSGDAKVRRHAADVFLAAGI
                     PTRKWEDYKYLHPEALFKKEFHGTPSVSRMVTEHDVKEMLIAPSAIRVVLVNGVFSAE
                     LSPKKNQPDGIVIMDLADAIVSSETAKKHYAKYATAEKDPFIAWNTALNEGGVFIHFP
                     AGAKMQEPVHIIHIASNEVSSVCHPRILIVAENGCEALIIESYESIGPVKTFTNAVTE
                     VFAAEHTKVHHYRVQAEGENAHQLNSLHALTQKGSNYSNYTFSFGGAWVRNNTNVVFS
                     QPFSEAHLFGLYLPGGKSVVDNHTVVDHAVPDCMSNELFKGIVNGKSTAVFNGKIFVR
                     PDAQRTNAFQTNRNILLSDDATVNTKPQLEIYADDVKCSHGTSTGRLNDEALFYLRAR
                     GIGEDNARKLLVRAFAEEVVEKVELKELRGYIDSRIDDLLA"
     gene            complement(3823..>4518)
                     /gene="sufC"
                     /locus_tag="VFU15_09365"
     CDS             complement(3823..>4518)
                     /gene="sufC"
                     /locus_tag="VFU15_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEU4718031.1"
                     /translation="NLEVKDGEVHAIMGPNGSGKSTLASVLAGREDYEVTEGEVLFNG
                     KNLLELSPEDRAREGLFLAFQYPVEIPGVSNINFLKTAISDIREYNGLPPVEAKDFLA
                     LVKEKQKLVEMDGKLAGRSVNEGFSGGEKKRNEIFQMAMLEPKLAILDETDSGLDIDA
                     LRIVANGVNKLRDGKRSFIVVTHYQRLLDYIVPDFVHVLYKGKIVRSGPKELALELEA
                     KGYDWIKEENIEA"
BASE COUNT          885 a         1238 c         1215 g         1180 t
ORIGIN      
        1 aggtcgatcg tgatcaaccc gctttcggct actttgtttt ttatttcttc catgttcttt
       61 tagtgagacg ggaatgttga gatgtgcttt acttcggtag taatctcatt gctcacagct
      121 ttgtcacata aaccccagct ccagctgcgc cacttcgctc atcatgtctt tttcccaggg
      181 cggatcgaag gtcacttcca cggttgccgc tcttacgcct tccacgtcac gcactttgtt
      241 ttccacatca ggcggcagtg attctgcagc cgggcagttc ggcgaagtca gcgtcatgag
      301 gatcttcacc tcgttgtcgt cgctcacgtc gatcccgtaa atgagaccca gttcccagat
      361 gtccacgggg atctccgggt cgtagcacgt tttgatcgcg tcgatgatct tctgcgcgag
      421 ctccgcgttt ttagctatga tggtggcagg cattttcagt tacggtttac gatttgtttt
      481 ttacgctcag cgcaagcgcg tccagcttca tctgtttgat catcgaaagc agcccgttcg
      541 agcgtgtggg cgaaagatga tctttcagtc ctatccggtc gacgaaatcc atcggcgcat
      601 ccacgatctc tttcggcgtg tgactggaaa gaacacgcac catcagcgcc acgagacctt
      661 tcgtaatgat cgcgtcgctg tcggcgtggt aaattacttt tccatccttc agctcggtgt
      721 gcagccacac gcggctctgg catcctttga tgaggcgatc gtccgttttc agtggttcat
      781 ccatgggcgc gagcgatttc ccgaggtcga taatgtactg gtacttatcg tcccactgat
      841 cgaacatcga aaactcgtcg atgatctcgt tctcggtttc aatgatgctc atttctttgc
      901 cttttcaaat acccggtccc gttcattctg ccacaataac gttccgttcc tgcgcacatg
      961 gaatgtcagg tcgttctccc cgtggaagtt tgctgtcgtg tcgtgcagga tgatcctgtc
     1021 ctcgtgcagc gaccattttc cgttgtattc gccgggaaaa tcggaccccg cccattctat
     1081 gagaagggaa aatgtgctgt cggcgttcag gataaggtct gcatgttccc tggcgttgcc
     1141 ccatggacca tcgaacagtt tttcctcgtg ctcatgcgac catttcccgg taagcgtacc
     1201 ggccggtgcg acctgctgcc tgcaggaaac ggctgaagca agcagcaaaa aaatatgcag
     1261 cacggttttc atgacagcat cttcaccgct ttcataagcg cggttattaa tgaatcgatc
     1321 tcttctttcg tgttgtacag tgcgaatgaa acgcgtaccg ttcccgggat gccgaaacgc
     1381 tgcatgagcg gctgcgtgca gtggtgtccc gtgcgtacgg caattccctg ctggtcgagg
     1441 atcgtgccca cgtcgaacgg gtgatgattt ccaactacga acgagatcac gcccgctttt
     1501 tcttttgccg tgccgatgat gcgcatgccg ggtatcgcgt tcagtttttc cgttgcgtaa
     1561 acaagcagct cgtgttcgtg cgcggcaatg ttcgccatcc cgatcgcatc cacgtaatca
     1621 agcgctgccg cgagtgcgat cgcaccttcg atgttcggtg tgcccgcttc gaaacgcagc
     1681 ggcccgctga tgtattccgt tttttcgaac gtgacggttt ttatcgttcc tccgccgacc
     1741 tggtaaggca tcatttcatc gaggatcccg tcgcgtacat acagcgcacc gatgccggta
     1801 ggtccgtaaa ctttatgacc ggaaaaacag tagaactcgc agccggtctc atttacattt
     1861 accgccatgt gcggcacggc ctgcgcgcca tcgataagaa cgggaatgtt gtgcgcgtgg
     1921 gcgagctccg tcagctcttt cgccgggttt acggtgccga gcgtattcga tacgtgcgcg
     1981 aaagcgaaga acttcacctt cggtgaaaag agtttctgca gcagcgcgat ctccagctcc
     2041 ccgtcgtcat tcaccgggag cacgcgcaac gccgcaccgc gttcttcgca cagcaattgc
     2101 cagggcagga tgttggagtg atgctccatt tccgtaacga gcacttcgtc gccgggctgc
     2161 aggcggttcc gcaggtagga ttgcgcaacg agattgatgc tttccgttgt tccccgcgtg
     2221 aagatgatct cgtccgggga attcgcacgg agatgcgcct gtacggtcgc acgtgcattt
     2281 tcatatgcct gcgaagcttc ctggctcagg tgatgaacgc cgcggtggat gttcgcatta
     2341 taattcgaat agtaatccgt gatgcagctg agaacggcct gcggtttttg agtggtcgcg
     2401 ccattgtcaa aatacacgag cggctttccg ttcacctggc gggagagaat gggaaattcg
     2461 ctgcgaagct gaagaatatc gtaagcggaa ccgggaatta tatgggagac ggttttggtc
     2521 atgatcaggc aagtaaatca tcgatgcggc tgtcgatgta gccgcgcaat tctttcaatt
     2581 caactttttc caccacttcc tctgcgaatg cgcgcacgag cagcttccgc gcgttgtctt
     2641 cgcctatgcc gcgcgcgcgc aggtagaaca gcgcttcgtc attcagccgg cccgtggacg
     2701 tcccgtgtga gcatttcaca tcgtcggcat aaatttcaag ctgcggtttc gtgttcaccg
     2761 tcgcgtcgtc gctgagaagg atattgcggt tcgtctggaa cgcgttcgtc cgctgcgcgt
     2821 cggggcgaac gaaaattttc ccgttgaata ccgcggtgga ttttccgttc acgatgcctt
     2881 tgaacagctc gttgctcatg cagtcgggaa ccgcatggtc gacgaccgtg tgattgtcca
     2941 cgacgctttt tccgccgggc aggtacaggc cgaagaggtg cgcttccgaa aacggctggg
     3001 agaaaacgac attcgtgttg ttgcggaccc aggcgccgcc gaaagaaaag gtataattcg
     3061 agtagttgct tcctttttgc gtcagtgcat ggagcgaatt cagctggtgt gcattctctc
     3121 cttcggcctg cacgcggtaa tggtgcacct tcgtatgctc agcggcaaaa acttccgtca
     3181 ccgcgttcgt gaatgttttc accgggccga tggattcata cgattcgatg atcagcgctt
     3241 cgcagccgtt ttccgcgacg atgagaattc tcgggtggca cacggaagaa acttcgttcg
     3301 atgcgatgtg aataatgtgc accggctcct gcatttttgc accggcgggg aaatgaatga
     3361 acacacctcc ttcattcagg gcggtattcc aggcgatgaa cggatccttt tccgccgtgg
     3421 catatttcgc gtaatgcttt ttcgctgttt cgctggaaac gatcgcgtcg gccagatcca
     3481 tgatgacgat cccgtccggc tgatttttct tcggggaaag ttccgcggaa aaaaccccgt
     3541 tcacgagaac gacgcggatt gcggaaggcg cgataagcat ttccttcaca tcgtgctccg
     3601 tgaccatgcg tgaaacggac ggcgtcccgt ggaattcctt tttgaaaagc gcttcggggt
     3661 gaaggtattt gtaatcttcc cacttgcgtg taggaatgcc ggcggcgaga aaaacgtcgg
     3721 cagcatgccg gcggactttc gcatcgccgc tgagggaagg aagcttcccg ggaaaacctt
     3781 ccatgagctt ttccgcaacc ggattttttt cagccactgt gctcatgctt caatgttttc
     3841 ttcctttatc cagtcgtatc cttttgcttc cagctcgagc gcgagctctt tcgggcccga
     3901 acgtacgatc tttcctttgt aaaggacatg aacaaaatca ggaacgatgt aatcgagcag
     3961 gcgctggtaa tgcgtgacga cgatgaacga acgtttgccg tcgcgcagct tgttcacgcc
     4021 gttggccacg atgcgcagcg cgtcgatgtc gagcccggaa tcggtttcat cgaggatagc
     4081 cagcttcggc tccagcatcg ccatctggaa aatctcgttg cgtttttttt ccccgccgga
     4141 aaaaccttcg ttcacggagc gcccggccag cttgccgtcc atctccacga gcttctgctt
     4201 ttcctttacg agggcgagaa aatctttcgc ttccaccggc ggaagtccgt tgtattcgcg
     4261 gatatcgctg atcgccgttt tcaggaagtt gatgttgctg acgccgggaa tttccaccgg
     4321 gtactggaac gcgaggaaaa ggccttcgcg tgcacggtct tcgggtgaaa gctcaagaag
     4381 atttttcccg ttgaaaagca cttcgccttc cgtcacttcg taatcttctc tcccggccag
     4441 caccgaggcg agcgtgcttt ttcccgatcc gttcggaccc atgatggcgt gcacttcgcc
     4501 gtctttcact tcaaggtt
//
LOCUS       DASTSF010000327         2133 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1120766,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000327 DASTSF010000000
VERSION     DASTSF010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1120766"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1650
                     /locus_tag="VFU15_09370"
     CDS             <1..1650
                     /locus_tag="VFU15_09370"
                     /inference="COORDINATES: protein motif:HMM:NF013636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HEU4718032.1"
                     /translation="SVTVSLHHTYDSDLDIWLIDPSNDSVNLSNNEGGGGDDFTNTVF
                     SMSATTSIQLGTAPFTGSYLPEISLNNLNNASDPNGTWRVCVADEAPQDTGHIIQVMI
                     SFCANPPHDPANFSGPCSMSNPAGCQCPDGSQDCDLLPDMLASGDIITQQHTETPGMI
                     TLSNATPNVGWGPMEIHGSNSCWCDSVNVPCTTTMCPNGNPPTQKLVQRIYHKNGNTI
                     TYHDTLTPGTMSYHPSHGHIHVNNWAEFTLRVRDTTIASPLNWPIVAHGSKVSFCLIN
                     LGDCTNDYGFCRDSAGNVITMADVPNAPFGLVSGCGVDQGIYTGNLDIYDQNLPDMFM
                     DLSGVCNGDYYIVSITDPDNNFIEQNDNNNWVAVPITLTMQHMPITGSFNITSVSGNS
                     ASFSSNNTDVTSFMWDFGDGSVDSTSNPTMHTYTYGGTYTVTLIQSNPCGTDTTSQVI
                     TITGLEERGDFSSQLLKATPNPAAGSTVISYNMPESGNMSLDVYDVLGNKVANLASGT
                     QAAGWHNETLDFNALGLADGSYFVRLVTPAHTSTMRVIRTQ"
     gene            complement(1701..>2133)
                     /locus_tag="VFU15_09375"
     CDS             complement(1701..>2133)
                     /locus_tag="VFU15_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018944889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2480 family protein"
                     /protein_id="HEU4718033.1"
                     /translation="VVLDIKAQLFQELILREKDFREFIKTNDWSQYKDKYVALTCSAD
                     AVIPDWAWMLLASALEPFAKKIVFGSTETMETVLFDEIISSLDISAYKDARVVIKGCG
                     DKPVPKTAYVDLTRRLRPVVKSIMYGEPCSTVPIYKQPKKQ"
BASE COUNT          502 a          611 c          551 g          469 t
ORIGIN      
        1 tccgtgacgg tcagtcttca tcacacgtat gacagcgatc tcgacatctg gctgatcgat
       61 ccttcgaatg actcggtgaa cctgagcaat aacgaaggcg gcggcggcga tgatttcacc
      121 aacaccgttt tctcgatgag cgcgacaacg tccatacaac tgggcacagc gccgttcaca
      181 ggaagctatc tcccggaaat ttcgctgaac aacctcaaca acgcttctga cccgaacgga
      241 acgtggcgtg tgtgcgtggc ggatgaagcc ccgcaggata ccggtcacat catccaggta
      301 atgatcagtt tctgcgcaaa tcctccgcat gatcccgcta acttttccgg accgtgctcc
      361 atgagcaatc ctgccggctg ccagtgcccg gatggttcac aggactgcga ccttcttccc
      421 gacatgctcg cttcaggcga catcatcacg cagcagcata cggaaacacc tggcatgatc
      481 acgctgagca acgctacgcc gaacgtgggc tgggggccga tggaaattca tggttccaac
      541 agctgctggt gcgattctgt gaacgtgccg tgcacgacaa cgatgtgccc gaacgggaat
      601 cccccgacgc agaaactcgt tcagcgcatt tatcacaaga atggaaatac gatcacgtac
      661 cacgatacgc tcacgccggg cacgatgagc taccatcctt cgcacggaca catccacgtg
      721 aacaactggg cggaattcac gctgcgcgtc cgcgacacga cgatcgcaag cccgctcaac
      781 tggcccattg ttgcgcacgg atcgaaagtt tcattctgcc tcatcaacct cggcgactgc
      841 acgaacgatt acggcttctg ccgcgattcg gcaggcaacg ttatcacgat ggcagatgtt
      901 ccgaatgcgc cgttcggtct cgtcagcgga tgtggtgttg accagggaat ttacaccggc
      961 aacctcgaca tctacgatca gaatctcccc gacatgttca tggatctttc cggtgtgtgc
     1021 aacggcgatt actacatcgt ttccatcacc gatccggata ataatttcat cgagcagaac
     1081 gacaacaaca actgggttgc agtgcccatc acgctcacga tgcagcacat gccgatcacc
     1141 ggaagcttca acatcacgtc cgtttccggg aattccgctt cattcagcag caacaacacc
     1201 gacgttactt cattcatgtg ggatttcggc gatggaagcg tggattccac gagcaacccg
     1261 accatgcaca cgtacacgta tggcggtacg tacacggtga cgctcatcca gagcaatccc
     1321 tgcggaacgg atacaacatc gcaggtgatc acgatcacgg gacttgaaga gcgcggcgat
     1381 ttcagctcgc agctgctgaa agccacaccc aatcccgcag cgggaagtac ggtgatctcc
     1441 tacaacatgc ccgagagcgg gaacatgtcg ctcgatgttt atgatgtgct cggaaataaa
     1501 gtcgcgaatc ttgcttcggg aacccaggcc gccggctggc acaatgaaac gctcgatttc
     1561 aacgcgcttg gtcttgcgga cggttcgtat ttcgttcgcc tggtcacgcc cgcgcacacc
     1621 agcacgatgc gcgtgatacg gacgcaatag aattgttcct cctaaaaaaa gaaacgctcc
     1681 gtttttgcgg ggcgtttttt ttattgtttt ttcggctgct tgtaaatggg aacggtggag
     1741 cagggctctc cgtacatgat gcttttcacg acgggacgaa gcctgcgcgt gagatcgaca
     1801 taggcggttt tcggaacggg cttgtcgccg cagcctttga tcaccacgcg cgcatctttg
     1861 taggctgaaa tatcgagcga tgaaattatt tcatcgaaca aaaccgtttc catcgtttct
     1921 gtcgatccga aaacgatttt ttttgcgaac ggttccagcg cggaagccag cagcatccac
     1981 gcccagtcgg gaatgaccgc atccgcggag caggtgagcg caacgtattt gtctttgtac
     2041 tgcgaccagt cgtttgtttt gatgaactcg cggaaatctt tttcgcgcag gatgagctcc
     2101 tggaaaagct gcgctttaat gtcaaggaca acc
//
LOCUS       DASTSF010000328         4399 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1814686,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000328 DASTSF010000000
VERSION     DASTSF010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4399
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1814686"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..531)
                     /locus_tag="VFU15_09380"
     CDS             complement(<1..531)
                     /locus_tag="VFU15_09380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718034.1"
                     /translation="MFYRTLFLLFIAYGFLWYDTNPFPGWKKQRVPTEKEADDRNSSG
                     NDWHVYIAGGNIRVARDSSGWLEGPRPPFDPDTIGEAAQKDPFRIPPMGCFRPVCSMR
                     VNDGWLFGTDHGEWGGCLRFTDSLGRSTYEIASGNFKSIISVSGRFFVVEGLMHMSMS
                     VGKIIELTEENGKWGVA"
     gene            647..1858
                     /gene="sucC"
                     /locus_tag="VFU15_09385"
     CDS             647..1858
                     /gene="sucC"
                     /locus_tag="VFU15_09385"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003007924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HEU4718035.1"
                     /translation="MNLHEYQGKSILKSFGVPIQEGIVADNPEKAVDAAKQLALETGT
                     KWWVVKSQIHAGGRGKGKIKGTEQRGVALAKSLDEVKSISANILGNVLVTLQTGEKGK
                     KVSKVLIAQDVYYPGAEEPKEYYMSVLLDRGKGRNIIMYSTEGGMNIEEVAEKTPHLI
                     FKEEIDPRVGLRDFQCRRIAFNLGVTGDAFKNMVKFVRALYTAYEAIDASLFEINPLF
                     KTSDGKIIAVDSKVTIDNNGLFRHPDIAEMRDTSEEDPTEVEAGSHGLNYVKLDGNVG
                     CMVNGAGLAMATMDIIKLAGGEPANFLDVGGTANAARVEQAFRIILRDPKVKAILVNI
                     FGGIVRCDRVAQGIVDAYKNIGNIQVPIIVRLQGTNAEEAKKIITDSGLKVYSAILLQ
                     EAADLVKQVVK"
     gene            2077..2736
                     /locus_tag="VFU15_09390"
     CDS             2077..2736
                     /locus_tag="VFU15_09390"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEU4718036.1"
                     /translation="MIDFSSILSESEFSEFGRSRQSRKVKKGEAIFLEGKSPSSVFYL
                     HAGKVKVFTTDAQGREQIIHLAKPGDLMGYRAMLGGDTYSCTGAAMEDTEVWVIPKET
                     FFDLLDRNAAFSHAVIKLLTAELRHAEHHLAGLARKPVKSRLAEVMLLLADTYGFRDD
                     GQTIDVSLTREEIAGLVGTATETTIRLLLSLRDEGFLATEGKKIRLLDVKGLRLLSEN
                     A"
     gene            2772..3602
                     /gene="bamD"
                     /locus_tag="VFU15_09395"
     CDS             2772..3602
                     /gene="bamD"
                     /locus_tag="VFU15_09395"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HEU4718037.1"
                     /translation="MVKRFAIFLVLAAAMLAGCNDFQKLQKSVDLNMKYDKAVEYYNK
                     GDFTKAQILFDELYSVFRSTDKAESVAYYLAYCDFKTGDYIMASYLFRTYFRTYPTSP
                     RASECLYMSAYCHYLVSPPYSLDQEDTYSAIEEFQYFIQMFPTDTARIGECNKMVDNL
                     HKKLEKKAFLNAKLYYDIEDYKAAITSLGLFNHDYPDSHYKEESDYLIAMSKYYLALN
                     SIDAKKAERVQDCIDACNDFLTKYKESEYTGDVKMTLEKAKKLQMKLSNDNKINSNQK
                     "
     gene            3621..3950
                     /locus_tag="VFU15_09400"
     CDS             3621..3950
                     /locus_tag="VFU15_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013665049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEU4718038.1"
                     /translation="MVDFKKNNAEPTTVTRDVRAIDGVTGNIYESVAIISKRANQVST
                     ELKEELTGKLNEFATHTDNLEEVFENREQIEISKFYERLPKPTSIATQEFLEGKVYFR
                     NPSKEQE"
     gene            4017..>4399
                     /locus_tag="VFU15_09405"
     CDS             4017..>4399
                     /locus_tag="VFU15_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012781305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HEU4718039.1"
                     /translation="MLNGKKIILGVTGGIAAYKAAFLVRLLVKAGADVRVIMTPAAHS
                     FITPLTLATLSKNPVLTNFTEGEKGEWNNHVEAGLWADLLLIAPCSASTLSKLAGGRS
                     DNLLTAVFLSARCPVAVAPAMDLDM"
BASE COUNT         1131 a         1154 c         1139 g          975 t
ORIGIN      
        1 cgccacgccc cattttccat tctcttctgt aagctcgatt atttttccca cggacatcga
       61 catatgcatt agtccttcaa cgacaaaaaa acgccccgaa acgctgatga tgcttttgaa
      121 atttccggaa gcgatttcgt atgtgctcct tcccagtgaa tcggtaaacc gcaggcagcc
      181 gccccattcc ccatgatctg ttccgaaaag ccatccgtca tttacgcgca tgctgcacac
      241 cggccggaaa caacccatgg gcggaatacg gaacgggtct ttttgcgccg cttcgcctat
      301 tgtgtccggg tcgaaaggcg gccgcggacc ttccagccac ccgctgctgt ccctggcaac
      361 acgaatattc ccgccggcga tataaacatg ccagtcgttg ccggacgaat tcctgtcatc
      421 cgcttccttt tccgttggga cgcgttgttt tttccagccc ggaaacgggt tcgtgtcgta
      481 ccataaaaag ccgtacgcga taaaaagcag gaacagggtt ctgtaaaaca tcttcacctt
      541 taaagatagc aaagcgattg ttagcttcct gtgaaaacaa aatcgggatt cgcaattccc
      601 aattagtaat tcccgatata tttgccttca tccaaaaaac agccgtatga atcttcacga
      661 ataccagggc aaatcgatcc tcaaaagttt cggtgtgcct atccaggaag gaattgtagc
      721 cgataatcct gaaaaggcag ttgatgctgc gaaacagctc gcactggaaa ccggcacgaa
      781 atggtgggtg gtgaaatcgc agatacacgc gggcggacgc ggaaaaggaa agatcaaagg
      841 gaccgagcag cgcggcgttg ccctcgcgaa atcactggat gaggtgaagt ccatctctgc
      901 gaatattctc ggaaatgtgc tcgtgacact ccagacaggc gaaaaaggaa aaaaagtaag
      961 caaggtgctc attgcgcagg atgtttatta tcccggcgcg gaagaaccga aagaatacta
     1021 catgagcgtc ctgctcgatc gcgggaaagg acggaacatc atcatgtatt ccaccgaagg
     1081 cggaatgaac atcgaggaag tggcggaaaa aacgccgcac ctcattttca aggaagagat
     1141 cgatccccgt gtcggccttc gtgatttcca gtgcaggcgc atcgcgttca acctcggtgt
     1201 tacaggtgat gcgttcaaga atatggtgaa attcgtccgc gcattgtata cggcgtatga
     1261 agccattgat gcaagccttt tcgagatcaa cccgctcttc aaaacatccg acgggaagat
     1321 catcgccgtc gattcgaaag ttaccattga caacaacggc cttttccgcc acccggatat
     1381 cgccgaaatg cgcgacacct ccgaagaaga tcccaccgag gtggaagcgg gcagccacgg
     1441 cctcaactac gtgaagctcg acggaaacgt gggctgcatg gtgaacggcg ccggacttgc
     1501 catggcaacg atggatatta tcaagctggc cggcggagag cctgcgaatt tcctcgacgt
     1561 aggcggaaca gccaatgcgg cacgggtcga gcaggcgttc aggatcattc tccgcgatcc
     1621 gaaagtgaaa gcgatcctcg tgaatatttt cggcggcatc gtgcgctgcg accgtgtggc
     1681 gcagggaatc gtggatgcgt acaagaatat cgggaacatc caggtgccca ttattgttcg
     1741 cctgcaggga acgaacgccg aagaagcgaa aaagatcatc acggattccg ggttgaaagt
     1801 gtattcggcc attctcctgc aggaagctgc tgatcttgtg aaacaggtgg tgaaataatt
     1861 ttttgaatga tctgctgaaa cggtctgcac ctgcaggccg tttttgtttt tctgtgctgg
     1921 cttggtttta cgtatgcgac tgcagatctt tcccgccggc tgaatcccgt ccatctgtcc
     1981 tgtgccggga aagtcgttcc tgcggcacgt gccgaaggcg tatgacttgc gtcatttacg
     2041 aaagaagacc gggtgaaggt tgtaaatttg ctgcgcatga tcgatttttc ctccattctc
     2101 agcgagagcg aattttccga gttcggacgt tcgcgccagt cacgtaaagt gaaaaaaggc
     2161 gaagccattt ttctcgaggg aaaatccccg tcttccgttt tctacctcca tgccgggaaa
     2221 gtgaaagtct ttacaacaga tgcgcaggga cgagagcaga tcatccacct cgcgaagccc
     2281 ggcgacctga tgggataccg cgcgatgctc ggcggcgata cgtattcgtg cacgggggcg
     2341 gcgatggaag ataccgaggt ctgggtcatc ccgaaagaaa catttttcga cctgctcgac
     2401 cggaacgcgg cattttcaca tgccgtgata aagctgctga ctgcggagct gcgtcatgcc
     2461 gaacaccacc tggccgggct tgcccgcaag cctgtgaaat cgcggctggc ggaagtcatg
     2521 ctgctcctgg ccgacactta cggtttccgc gatgacggcc agaccatcga tgtttcgctc
     2581 acgagggaag agatcgcggg acttgtcggt acggccaccg aaaccacgat ccgcctgctg
     2641 ctcagcctgc gcgacgaagg tttcctggca acggaaggga agaagatacg tctcctcgat
     2701 gtaaaaggac tcaggctgct ttccgaaaac gcctgattca gccgaaaatc gtatctttgc
     2761 gccttgtttt catggtaaaa cgtttcgcca tattccttgt tcttgcagct gcgatgctcg
     2821 caggatgcaa tgatttccag aagctccaga agagcgtcga ccttaacatg aagtatgata
     2881 aggccgttga atattacaat aaaggtgatt ttaccaaggc gcagatcctg ttcgacgagc
     2941 tgtactccgt gttccgcagc accgataaag ccgaaagcgt ggcgtattac ctcgcttact
     3001 gcgacttcaa gaccggcgat tacatcatgg cgagctacct tttccgtacg tatttccgca
     3061 cgtacccgac cagcccgcgc gcttccgaat gtctctacat gagcgcttat tgccactacc
     3121 tcgtttcgcc cccgtattcg ctcgaccagg aagacacgta ttcggcgatc gaagagttcc
     3181 agtattttat ccagatgttc cccacggata cggcccggat cggtgagtgc aacaagatgg
     3241 tggacaacct gcacaagaag ctggagaaaa aagctttcct gaacgcgaag ctgtattacg
     3301 atatcgaaga ttacaaagcg gcgatcacgt cgctcggact ttttaaccac gattatcccg
     3361 attcgcatta caaggaagaa tcggattacc tgatcgcgat gtccaaatac tatcttgcgc
     3421 tcaacagcat cgatgccaag aaagcggaac gtgttcagga ttgtatcgac gcatgcaatg
     3481 attttctaac caaatacaag gaaagcgaat atacgggcga tgtaaaaatg acgcttgaaa
     3541 aagcgaagaa gctgcagatg aagctttcca atgacaataa aataaactct aaccagaaat
     3601 aacaatcaat ccggataaac atggttgact tcaaaaaaaa caacgcagaa cccaccactg
     3661 ttacacgcga tgtacgcgct attgacggtg tgaccggcaa catctacgaa agtgttgcga
     3721 tcatttccaa gcgcgccaac caggtgagca cggaactgaa agaagagctc acgggaaagc
     3781 tgaacgaatt tgccacgcac accgataacc tcgaagaggt gttcgagaac cgcgagcaga
     3841 tcgagatctc caaattctat gagcgtcttc ccaaaccgac gagcattgcg acacaggaat
     3901 tcctcgaggg taaagtctat ttccgcaatc cttcaaagga gcaggaataa ttttaccggg
     3961 cacgttttta cggacatggc ggttacggct gccatgtctt tcctttttat agcggcatgc
     4021 tgaacggaaa gaaaataatc ctcggtgtta caggcggaat cgcagcgtac aaagctgcct
     4081 ttctcgtgcg cctgctggtg aaggccggtg cggacgtacg cgtgatcatg acgcccgcgg
     4141 cgcacagctt catcacaccg cttacgctcg cgacactttc gaaaaatccc gttctcacga
     4201 attttacgga aggcgaaaag ggcgaatgga acaaccacgt ggaagcaggc ctgtgggccg
     4261 atcttctgct gatcgcgccc tgcagcgcat cgacgctttc aaagctggcc ggaggccgca
     4321 gcgataatct cctgacggcg gtttttcttt ccgcacggtg cccggttgcg gttgctcccg
     4381 cgatggacct cgacatgta
//
LOCUS       DASTSF010000329         4009 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1579323,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000329 DASTSF010000000
VERSION     DASTSF010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4009
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1579323"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..362
                     /locus_tag="VFU15_09410"
     CDS             <1..362
                     /locus_tag="VFU15_09410"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEU4718040.1"
                     /translation="NPVPHVVDEDGPEEVFAYVQEMPGFPGGPYALQKFLAKNIRYPE
                     MEKENGVQGTVFVSFTVMKDGTVGDVKVVKGVPGGPGLGKEAVRVISQMPAWAPGKQN
                     GRAVNVRMMQPVKFVLQ"
     gene            1178..1708
                     /locus_tag="VFU15_09415"
     CDS             1178..1708
                     /locus_tag="VFU15_09415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718041.1"
                     /translation="MDIYYSTEDKYIRAVQKLDYGYAAKAKRLLSEILEEEPDNGRAH
                     YQLARIAWWKLRDAEMTRRHLYYAKRFAPGFTPAWYFLVNVLLETRAHEQLLAVAAEA
                     ITVPGISHSWVYFLCALSHEQQLQLGEATRFAEKALQHSICEDESGEVKLALARIRRK
                     REAAKPFLYDTGTATD"
     gene            1728..1880
                     /locus_tag="VFU15_09420"
     CDS             1728..1880
                     /locus_tag="VFU15_09420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718042.1"
                     /translation="MKKNKYSRFVLLSSAPFCAYGNRIAFPRKKNPLGAIMGFVRSCL
                     AKGNGR"
     gene            2075..3136
                     /locus_tag="VFU15_09425"
     CDS             2075..3136
                     /locus_tag="VFU15_09425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718043.1"
                     /translation="MSKSATIFFAIFFPLAGVMLYHFFHPKGYTMRTGNVNIDNPTAQ
                     SLVMYIGKDTVQLPPFTFITDYPIASDSTNRIVVKTKDGKVLCDSIIPKDTYGMFLNP
                     THSKYVKWVIRYSSEDEELPDDSLFTDFHFIDSSLFIGNITVMNQLCIKGDDILDKLA
                     NEREVKSTTTMLPGQKHMDETHILRMEDFKTLYNKVSNVSDLDDAELKRHNAMVEYYN
                     NGLDHLPDSDPVLRWFGYELNSYPFVSGSEIDQAKREWEENDKYKRLDYYDKVVEAMK
                     EYDKVKAEGSDDDMPQDSTGVLSPMTCLQYNFDIHNGFFSSDAHVRVYEKKVYDPVSN
                     ESKTTYYHYYGSTPDSTTY"
     gene            3338..>4009
                     /locus_tag="VFU15_09430"
     CDS             3338..>4009
                     /locus_tag="VFU15_09430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718044.1"
                     /translation="MEFHGTVGTAHVRLLAEQHRNRFCGIYYYEKIRRTIYLSGITTP
                     GGVHFDELTLRDTFDEKVFYSVTDETDTTGAFDGAFTADSSQFTGQWVSHDGKKKFPF
                     SLSRDTIAQMDLPEIVHALRTCRSEGELHFLLCTQLVKTLDNFSFFTEGGGDWDTTQV
                     IGPQNMQVKLFTKNLFGNDSAEKLLYVNFCGSETFLFAFYKCAGRWKKIPGCVFEKQG
                     DESNRP"
BASE COUNT         1035 a         1019 c         1077 g          878 t
ORIGIN      
        1 ataaccctgt tccgcacgta gtggatgaag atgggccgga ggaagttttt gcgtacgtcc
       61 aggaaatgcc tgggtttccc ggcggacctt acgcgctgca gaagttcctc gcgaaaaata
      121 tccgttaccc ggaaatggag aaggaaaacg gggtacaggg caccgtgttc gtttcattca
      181 ccgtgatgaa agacggaacg gtcggcgatg tgaaagtggt gaaaggtgtt cccggcggac
      241 ccggtctcgg caaagaggcc gtgcgcgtga tctcgcaaat gccggcatgg gcgcccggga
      301 agcagaacgg tcgcgccgtg aatgtgcgca tgatgcagcc tgtgaagttt gtgctgcagt
      361 gaaagcagct ttgcatggcc cgggtaattt ttttcagaag gattcaaaaa aagaaatgaa
      421 atcgcttgac ttgtattgtt tcatgcggtt atatttgccg ctcaataaaa aaatcaaatg
      481 aaaaccattc tcacatatcg taaagcagga acagccggag ctgcagtgca gcttccctgt
      541 cttgtccgcc gaatgtgcgc atggtagaat catacacttc cagcactgca cccggcgaca
      601 aaacgccggg tgttttgctt tatacggaac catgcaaaga gaattgcata cgcacataag
      661 aaacagtgcc ggtgcacgga cgaatatcgc cgtgctcagc gcaacgggtc tgcctggggc
      721 ccgggtggga ctgctatgtc ccggcggata ggaaactatc cttccgacag cacccggtcc
      781 tgcaggcagc acgaccgggt ttttttatgg cagaaaaacg aatgcataaa agcaaaacgg
      841 aaaacaaaat gatcatcaac atcctcttca tcgcgtcgct cgttgttttc gtgcggaaca
      901 ctttgaagaa caaaggctga aatgccatgc acattttcat attgatcctg cacatcatca
      961 tcgcgcccgg gcgcatggcg gacgcaggag cgaaaaacaa aacacgcaaa cactgaaaaa
     1021 agaaaggagg ttatcatgaa cgctgaaaga gctgtaacaa ttctgatcac accctgccag
     1081 ggagttgcgg gagcggtaac cgaaacgccg ccccgtgcgg gcctgccgcc ggatgaaaac
     1141 ggcggcaggc tgttgtcaca cagaaaacaa aactgaaatg gatatttatt attcgaccga
     1201 agacaaatac atccgcgcgg tgcagaagct ggactacggt tatgcggcca aagcgaaacg
     1261 gctgctcagc gagatactcg aggaagaacc ggacaatggc cgcgcacatt accagctcgc
     1321 gcgcattgcg tggtggaagc tgcgcgatgc ggaaatgacc cggcggcacc tgtactatgc
     1381 gaaacgcttt gcgccgggat tcacaccggc ctggtatttc ctggtgaacg tgctgctcga
     1441 aacacgtgcg catgaacagt tgctcgccgt cgccgccgag gcgataaccg ttcccgggat
     1501 ctcgcattcg tgggtgtatt tcctgtgcgc gctttcgcat gagcagcagc tgcagcttgg
     1561 tgaagccacg cggttcgcgg agaaggccct gcagcattcg atctgcgagg acgaatcagg
     1621 cgaggtgaag ctcgcgcttg cgcgcatacg gcggaaacgg gaagccgcaa aaccgttcct
     1681 gtacgatacg ggaacagcaa ccgattaaaa aagaaagaag gcaggtcatg aaaaaaaaca
     1741 agtacagcag gttcgtcctg ctcagcagcg cacctttctg cgcatacgga aaccgtattg
     1801 catttccgcg gaagaaaaat ccgctcggcg cgatcatggg atttgtcagg agctgcctgg
     1861 caaagggaaa cgggcggtga gtgttcctgt gggttctctg cccgcttccg gaatgcatac
     1921 gccctgtttt gcgactccgg ggttttgcgg taaaagaaat gagccacaaa ccccggagtt
     1981 ttagggtaaa aaattcaccc ggacggttgc atggattcac tttttcgcga tcataatatt
     2041 tgaattatct taacgcctct aaacccttgc cctcatgagt aaatccgcta ctattttctt
     2101 cgcgatcttt ttcccgctcg cgggagtcat gctgtaccac ttttttcacc cgaaaggata
     2161 caccatgcga accggcaacg tgaacatcga caatcccacg gcgcaatccc tggtcatgta
     2221 tatcgggaaa gacaccgtgc agcttccgcc gttcactttt attacggatt acccgatcgc
     2281 atccgattcc acgaaccgca ttgtcgtgaa aacaaaggat ggaaaagtgc tctgcgattc
     2341 cattatcccg aaagacacgt atggtatgtt cctcaatccc acgcattcga aatatgtgaa
     2401 gtgggtgatc cgctattctt cggaggacga ggagctgccg gatgattcgc tgttcacgga
     2461 ttttcatttc atcgacagct cgcttttcat cgggaacatc acagtgatga accagctttg
     2521 catcaaggga gatgacattc tcgacaagct cgccaatgag cgcgaggtga aatccaccac
     2581 cacgatgctg cccggccaga agcacatgga tgaaacgcat attctccgca tggaggattt
     2641 caaaacgctt tacaacaaag tttccaacgt ctcggatctc gatgatgcgg agctgaaacg
     2701 ccataatgcg atggtggaat attacaacaa cgggcttgat cacctgccgg acagcgatcc
     2761 cgtgctccgc tggttcgggt acgagctgaa cagctatccg ttcgtttccg gcagcgagat
     2821 cgaccaggcc aaacgggaat gggaagaaaa cgacaagtac aaacgcctcg attattacga
     2881 caaggtggtt gaagccatga aagaatacga taaggtgaaa gcggaaggca gcgacgacga
     2941 tatgccgcag gattcaacag gcgttctctc cccgatgacc tgcctgcagt acaacttcga
     3001 cattcacaac ggctttttct ccagcgacgc gcacgtgcgt gtgtacgaga agaaagtata
     3061 cgaccccgtt tcgaacgagt cgaaaaccac gtactaccat tactacggct caacccccga
     3121 ttccacgacg tactgatcgg ggagatttta cacctgctca aaaaagaccg gcacctgccc
     3181 gttcagtaac agggcatagt gcggaagtct gcatgtttgc attcggatga acacgaagca
     3241 cattcggttt tttctggagt actctatcgc ggcggcagtc gctttcctgt tctctcttca
     3301 gctgtccgca gcgggttaca ccagcgaagg aaacctggtg gaatttcacg gtaccgtcgg
     3361 aacggcgcac gtccgcctgc tggcggaaca gcaccggaac aggttttgcg ggatctatta
     3421 ttatgaaaaa atccgcagga ctatttacct gtccgggata acaactcccg gcggagttca
     3481 tttcgacgag cttacgctgc gcgatacgtt cgacgaaaaa gttttttatt ccgttaccga
     3541 cgagacggac acgaccggcg ctttcgacgg ggcgttcacg gccgattcat cgcagtttac
     3601 gggtcaatgg gtttcgcacg acgggaagaa aaaatttcct ttcagtcttt cccgtgatac
     3661 gatcgcgcaa atggacctgc ctgaaattgt gcatgcattg cgcacctgcc ggtcggaagg
     3721 cgagctgcat ttcctgctgt gcacgcagct ggtaaaaacg ctcgataatt tttcgttctt
     3781 caccgagggc ggcggcgact gggatacgac gcaggtgata ggtccgcaga acatgcaggt
     3841 gaaattgttc acgaagaacc tgttcgggaa tgattctgcg gaaaagctgc tgtacgtgaa
     3901 tttttgtggc agtgaaacct ttctttttgc attttacaaa tgtgcgggca gatggaaaaa
     3961 aattcccggc tgtgtttttg aaaagcaggg agatgaatcg aaccgtccg
//
LOCUS       DASTSF010000330         2692 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1405926,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000330 DASTSF010000000
VERSION     DASTSF010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2692
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1405926"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..187)
                     /locus_tag="VFU15_09435"
     CDS             complement(<1..187)
                     /locus_tag="VFU15_09435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718045.1"
                     /translation="MNILFRKAGPDDAALLRRLAAEAFYNAYAKFNMETDMKMYMETY
                     FTEERVQAEISDPHLYFL"
     gene            complement(573..>2692)
                     /locus_tag="VFU15_09440"
     CDS             complement(573..>2692)
                     /locus_tag="VFU15_09440"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HEU4718046.1"
                     /translation="NEGLSELTYYTVPGSPEKRFSIKNYTTADGLGSNVILSLYEGDD
                     GVLWVGTGFGGVSKLAPGSAHFETYSTKDGLAGDVVYAINSDTHGNIWIGTKEGASRF
                     DPATKVFRNFTTEDGLGGNNVYRIFRDSQGKMWFGALGGYLSVYDGKGFTKYDETSGI
                     VHRFILSINEDKEHNIWFGAYGGGLYKFDGRTFHNYTTRNGMTTDSPYAIIADDENNI
                     WIGNGRGLDRFNPADSSFSHYGRSEGFPGVEVNPNAVAKGPDGCLWFGTIMGVVKFDP
                     QENRKNEIEPLTSLIGLKIFMRDADFPENARFSYDQNHLTFTFVGLSLSNPEKVRYQY
                     KLDGFDKDWSPVPTEHNFAVYSNLSPGEYTFEVRASNNDGVWNKEPVKYKFFIRPPFW
                     QTATFYVLVVLFILFCIFGFDRWRTTNLKKQKKILETKVEQRTLELALRNEELAQKNK
                     EITDSIRYAKRLQDAMLLPSREFRKAVPDSFILYKPKDIVSGDFYFSRIVKSGGKSRL
                     VLAAVDCTGHGVPGAFMSIITNDIILQVIEGHESESPAQLLDRLSKSMSEKMRHTIDD
                     IVVRDGVDIALAIIDLDTLVLRYAGAFNPLYHFRGKQFTEFKADKISIGVLADDIQKN
                     YTDQEIQLQSGDTVYLFSDGYADQFGGPQGKKFKLTQLKALFLSMQDQDMPDQLRLLD
                     QTIETWKGSHEQVDDMLVIGVRF"
BASE COUNT          610 a          723 c          700 g          659 t
ORIGIN      
        1 gaagaaaata caaatgcggg tcggaaattt ctgcctgcac ccgttcttcc gtgaaatacg
       61 tttccatgta cattttcatg tccgtttcca tgttgaattt tgcgtaagca ttgtaaaatg
      121 cttctgcagc caaacgccga agaagcgctg cgtcatccgg acccgccttg cgaaacagga
      181 tattcatact gttgtttttt tttaccacga agaacacaaa ggtccgcaaa gaaatttatt
      241 aaagaaccat cggaatatct tcgctatttt tttcaccacg aagaacacga cgctttttca
      301 caaagcacac gaagaaaaac gaacacaatc agaacaccgg acccggaact aaaaaccatt
      361 ttaaccacga agaacacgaa agacctgtgg cggaacgcaa aagtccatga aaaaatttca
      421 aaaacgggca gggatattcc tgttactcac attccccgtc ggcaccggaa ttccggtcgt
      481 acactttttc gcaaagatga ataccagacc gcggcttgcg acaggcagcg atgatcgcaa
      541 aaaatgggct tgcaatagtt cacgtccatt tcctaaaacc gcacgccgat cacgagcatg
      601 tcgtctacct gctcatgcga tcctttccag gtttcgatcg tctgatcgag caggcgcaac
      661 tggtcgggca tgtcctggtc ctgcatggaa aggaacaacg ccttgagctg ggtgagtttg
      721 aatttttttc cttgcggtcc gccgaactgg tcggcataac catccgagaa aagataaacc
      781 gtgtcgccgc tttgcagctg tatctcctgg tcggtgtaat ttttctgaat gtcatcggcc
      841 agcacaccga tggagatctt gtcggctttg aattcggtga actgctttcc gcggaaatgg
      901 tacagcgggt tgaatgcgcc ggcatacctg agcacgagcg tgtcgaggtc gatgatcgcc
      961 agcgcaatgt ccacgccgtc gcggacgacg atatcatcga tcgtgtggcg cattttttcg
     1021 ctcatgcttt tgctgagacg gtcgagcagc tgcgcgggcg attcgctttc gtggccttcg
     1081 atcacctgga ggatgatgtc gttcgtgatg atgctcatga atgcgcccgg aacgccgtgg
     1141 cccgtgcaat cgaccgcggc aagaacgaga cgcgattttc ccccgctctt cacgatgcgc
     1201 gagaaataaa agtcgccgct gacgatgtcc ttcggcttgt acaggatgaa ggaatccggt
     1261 acggctttgc ggaattcacg gctcggcagc agcatggcat cctgcaggcg tttcgcgtag
     1321 cggatgctgt cggtgatctc cttgttcttc tgcgcgagct cttcgttgcg cagggcaagc
     1381 tcgagcgtac gctgctccac ttttgtttcg agtatctttt tctgcttctt caggttcgtc
     1441 gttctccagc ggtcgaaacc gaaaatgcag aacaggatga acagcaccac gagcacgtag
     1501 aacgtcgccg tctgccagaa cggtggacgg atgaagaatt tgtatttcac gggctccttg
     1561 ttccacacgc cgtcgttgtt cgacgcgcgc acctcgaacg tgtattcgcc cggggaaaga
     1621 ttggaataca cggcgaaatt gtgctcggta ggaacgggcg accagtcttt gtcgaagccg
     1681 tcgagcttgt actggtagcg cactttttca ggattcgaaa gactgagccc gacgaacgtg
     1741 aacgtaaggt gattctggtc gtagctgaat cgcgcattct cggggaaatc cgcgtcgcgc
     1801 atgaaaattt tcagcccgat gagcgacgtg agcggttcga tctcgttctt cctgttttcc
     1861 tgcggatcga atttcacgac gcccatgatc gtgccgaacc agagacatcc gtcgggtcct
     1921 ttcgccaccg cgttcgggtt cacttcaacg ccgggaaatc cttcggaacg gccataatgg
     1981 gaaaacgagc tgtcggcggg attgaaacga tcgagacctc ttccgttccc gatccagatg
     2041 ttgttctcgt catccgcaat gatcgcgtac ggggaatcgg tcgtcatgcc attcctcgtc
     2101 gtgtaattgt ggaacgtccg cccgtcgaac ttgtacaatc ctccgccata ggcgccgaac
     2161 cagatgttgt gttccttgtc ttcgttgatg ctgagaatga accggtggac gatgccgctc
     2221 gtttcgtcgt atttcgtaaa tcctttcccg tcgtagacgg acaaatatcc gccgagcgcg
     2281 ccgaaccaca tttttccctg gctgtcacgg aaaatgcggt agacgttgtt cccgccgaga
     2341 ccgtcttccg tggtaaaatt gcggaacact ttggtcgccg ggtcgaaccg cgatgcgcct
     2401 tccttcgtcc cgatccagat atttccgtgt gtgtcggaat tgatcgcgta aacgacatcg
     2461 ccggcaagac cgtccttcgt actgtacgtt tcaaaatgcg ccgagcccgg tgccagctta
     2521 ctcacgccgc cgaagccggt tcccacccaa agcacgccgt catcaccttc gtaaagtgaa
     2581 agaatgacgt tgctgccgag accatctgcc gtcgtgtaat ttttaatgga aaatcttttc
     2641 tcgggcgaac cgggaacggt gtaataggtc agctcggaaa gaccttcgtt cg
//
LOCUS       DASTSF010000331         7468 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_976956,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000331 DASTSF010000000
VERSION     DASTSF010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 7468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7468
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_976956"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1060
                     /locus_tag="VFU15_09445"
     CDS             <1..1060
                     /locus_tag="VFU15_09445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718047.1"
                     /translation="HLRLDSLVGPRPSHGNGVSPDLKNDPAKKAGEQEEKRAALLQDS
                     LLRADSVAMAVKETRKDSVMNDSTMCAELTDAAVRPDSSGAVVLPVFFIAFDGISDNA
                     KAGTPKYQKTDPQSGDVNVYDEQTLKSDRQQTWTFGARAGYFISRHFALLAGGNASRY
                     VAPQGTGNLKFNHNHEYLLNFHTATSSFQLPSGSFTADDSNTQQGDTFHISVQSYERY
                     RYFNLRLGGAFYPVRNRYFSIYIDAFCNGAYLTKQDLTLTVPKSGKTFSFSGDELSGM
                     NRFTIGWQAGAGFEWMPVKNLGIWAEPSWFSFGTLNKKNAVMIDPSSFRVQFGLAWHF
                     GYPYHMAASGSKVYRDGN"
     gene            complement(1449..2078)
                     /locus_tag="VFU15_09450"
     CDS             complement(1449..2078)
                     /locus_tag="VFU15_09450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718048.1"
                     /translation="MIRLLFLFALLITAACGQPSAHETASTGTKFGVRYTFKKYVNDT
                     PPLSYFVFDITLENNSDSARWFVLPGECGPGDFLSNAPSSVRIGRYCNDSCVYIGQIW
                     HDEESQNCNLILLGPSSKITLLNFDIGDKRGDNDNKPFPLTTLVCRNVSIDSIRFEKF
                     WPVSLLCHGGYDVDFAGSNSLKHLDSRKVSPFAALRFDTLVSLNTTIRP"
     gene            complement(2075..3988)
                     /locus_tag="VFU15_09455"
     CDS             complement(2075..3988)
                     /locus_tag="VFU15_09455"
                     /inference="COORDINATES: protein motif:HMM:NF039793.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HEU4718049.1"
                     /translation="MLDEEEAQRKKNADAKGRPGSSPLTVSNPENASEKEADAVAQKV
                     ASGQRLDFSTPVTSTFAIQAKGQEDLLPSADPIRSSLSTSKGSGRPLDDSAKMEMETT
                     MGTDFSNVRIHTGANAHTMSESINARAFTHGQDIYFRDGHYNTQSKEGKELLAHELVH
                     TKQEAGSDNAIHRKPDDKSPDRGASAGANGSFNTKTGGYSVADENDNLNAIAKRFNTT
                     VYELRKLNNLIPTDSDDFAKNNANYKLKSGQVLKIPSSATYAGNVSKTLNAPADLVGK
                     LKDLNYDWGATTGVHYAAPYKENVEDYKNDPVKNANLKPYADNWQSQFDYGYANENYW
                     QLTTDKIYYFRIKPGVKASEALKAWFNGLTIADCASAVIAIYYDNLRQALGDERFDEV
                     FSDMPPYNASPDSKAPLVISQYTSETYFKQFVFDANKERKLGAENDRPVKIGDWCYFN
                     NHPAYKLKHPGGNFKGENAVYEGRKTYWDPDLKKDVSMQLWSGFGVSHATEKELRADM
                     AEAYNFERNEDDYRRIIMDILPALPKKVQNEINTSKSSYKDIYNDHLDYIPKFLQEGQ
                     HFFPSSILPEDILNAEPITSDKYPISILATLKVIDERARAIYSASTKNKGGFAPEQVR
                     RLDFDAAVTNLKK"
     gene            complement(4218..5972)
                     /locus_tag="VFU15_09460"
     CDS             complement(4218..5972)
                     /locus_tag="VFU15_09460"
                     /inference="COORDINATES: protein motif:HMM:NF014589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase N-terminal
                     domain-containing protein"
                     /protein_id="HEU4718050.1"
                     /translation="MNILGFNCYGHDSAAALMVDDEVVFAVEEERLNRKKHYGGIPEA
                     SIRAALEHANLTLADIDHVAFFWKPSISYSKVPVFLLKFWNKVPQLLREQRSFSVEEN
                     LGMLNYLKDMKRLPGTLKKLFPSEREAKFRFHLLEHHFCHAASAFFPTPYDDAAILTI
                     DGAGEWTTSMFAHGKGNDIRRIGGVNTPYSLGAFYQAISRHLGFRLIEGPGKLMGLAS
                     YGKADTDVYRKMRSLVKLTGDGGFRLDLSYFCYHYTRKTGVTKKFTDLFGASKSEGKD
                     WSGHELDVAAAAQHIVEDVILHMVRTLKKNTGSENLCMAGGVALNSVTNGLIAKEGLF
                     KNIFIQPAAGDSGTALGAALMLNHGVLKRERKWIMETAFLGPGYATEDYEAALKKSGL
                     PYVRTDDYCAFAARQLAKNKILGWFQGRMEFGPRALGNRSIITSPLGENTKAVVNARV
                     KFREPFRPFAAIVLEEDCGKYFDSSFPNPYMLFVYNVRPEYLGKMPAITHVDNSVRIQ
                     TVNATENPEMRKLLEAFKKETGYSVLLNTSFNIKGEPIVASPEDAVHSFERADMDYLI
                     IGDFIVGKKGDEGSLREV"
     gene            complement(5978..>7468)
                     /locus_tag="VFU15_09465"
     CDS             complement(5978..>7468)
                     /locus_tag="VFU15_09465"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU4718051.1"
                     /translation="PPHWTSGGGAPLVYLQTQAWVIAHYLYTFFIPNQLSADTDWTLI
                     TNMFDLRILAGAGIIIALFITAVQLSGKNETKGISFGIAWFFIALAPTSSIFSFAEVL
                     NDHRVFFPFIGLVFAVASIAMLILEKTEQQGKNIPVRAGLSVFAVVLLLAHAAGTHER
                     NHVWSSGETLWKDVTEKSPDNGRGWMNYGLSLMAKSDYDGAIDCFNRGLKTYPYYSYL
                     YINMGIAENGRKNTEAAEKNFRYALSLDSLNPESYYYYGDFLVQHERMDEAKAILEKG
                     KTVSPQHEGINNLLSLFANGTVHTRLDMAIDAAQKNPTADNYVLLSLAYYNDGQYAQS
                     AGAALKAAAINPAYANAWNNACAAYNKMGEFDEAIAAGQKAVQLAPTDALSKNNLAFA
                     LSQQAHFTQLEDSLRKNPSYTGYVSLSMQWYAAGNYGRALAAGQQAVKINPGDATGWN
                     NICAYANALHEWDIAIDAGKKALKIKPDFQLAKNNLDIALKGKGTR"
BASE COUNT         1635 a         1905 c         1965 g         1963 t
ORIGIN      
        1 tcacctgcgg ctcgattcgc tggtggggcc acggccttcc catggaaacg gcgtatcgcc
       61 cgatctgaaa aatgatccag cgaaaaaagc aggagaacag gaagaaaaga gagccgccct
      121 gctgcaggat tcacttttac gtgccgattc agtggcaatg gcagtgaagg aaacccggaa
      181 ggattccgtg atgaacgaca gcacaatgtg cgcggaactg actgatgccg ctgtacgtcc
      241 cgattcttcc ggtgcagtcg ttctcccggt atttttcatt gcctttgacg gcatcagcga
      301 caatgcaaaa gccggtacac cgaaatacca gaagacagat ccgcaaagcg gcgatgtgaa
      361 tgtgtatgac gagcaaaccc tgaaatcgga caggcagcag acgtggacgt tcggtgcgcg
      421 cgcggggtat tttatttccc gtcatttcgc gctgctcgcc gggggaaacg cttcgcgtta
      481 tgtggcgccg cagggaacag gcaatttaaa attcaaccac aaccacgaat accttttgaa
      541 tttccatacc gccacttcct cgtttcagct cccgtccggc tcgttcacgg cagatgattc
      601 gaacacgcag cagggcgaca cgttccacat cagcgttcag tcgtacgagc gttaccgtta
      661 tttcaacctg cgtctcggcg gcgcattcta tcccgtacgg aaccggtatt tcagcattta
      721 catcgatgcg ttctgcaacg gcgcttatct cacaaagcag gatcttacgc tcaccgttcc
      781 gaaatccgga aaaacgtttt ccttcagcgg tgacgagctg agcgggatga atcgcttcac
      841 gatcggctgg caggccggtg caggtttcga atggatgccg gtgaaaaatc tcgggatctg
      901 ggctgaacct tcctggttct ctttcggaac gctcaacaaa aagaacgcgg tcatgatcga
      961 tccttcttcg ttccgcgtcc agttcggact tgcctggcat ttcgggtacc cgtatcacat
     1021 ggccgcttcg ggttcaaaag tgtaccgcga cggaaactga gttgttgctc actgtgcaaa
     1081 agcaggcgag gctggaattt tatgcagtaa cgtgatgggg ggccggccaa aagaaaaaag
     1141 ccggtccgtt ctcccaaccg gaccagcttt tggaaacccg gtgaaattcc tttcggaatt
     1201 tccaggcaaa ataaagaagg ctaccctttt tgcctgttcc gggttagatc aaattgtgca
     1261 ggtataccta accttacaag tgcctgctga tgataagatg cggcaggtgg acgggaggtt
     1321 gcccgggaaa taaaaaaagt ggaaaagatg ccctgttgcg attttcgctt cagatcagca
     1381 tcgggcattc cgtcagggcc tttttaagtt ctggcggaag cccgaaatgt tccccggggt
     1441 gaaaattatc agggcctgat ggttgtattc agcgaaacca gcgtgtcaaa ccggagcgca
     1501 gcgaaggggg aaactttcct gctatccagg tgcttaaggg aattcgatcc ggcaaaatcc
     1561 acgtcatagc ctccatgaca gagaagggat acgggccaga atttttcaaa gcgaatggaa
     1621 tcgatgctca catttctgca cacgagcgtg gtgagtggaa aaggcttgtt gtcgttgtct
     1681 ccgcgcttat caccaatatc aaaattcagc agcgtaattt tcgatgacgg tccgagaagg
     1741 ataaggttgc agttttgaga ttcttcgtca tgccagatct gcccgatata tacacagctg
     1801 tcattgcagt accgcccgat gcggacggat gaaggagcgt tactgagaaa atctccgggg
     1861 ccgcattcgc cgggcagcac aaaccatctt gccgaatcac tgttattctc aagggtaatg
     1921 tcaaacacga aatagctgag cggcggagta tcatttacgt attttttgaa agtgtaccgg
     1981 acgccgaact tcgtacccgt tgaagccgtt tcgtgtgcgg aaggctgacc gcaggcggca
     2041 gttatcaata gtgcaaataa aaacagtaag cgaatcattt tttcaggttt gtaacagccg
     2101 cgtcaaagtc caatctcctt acctgttccg gcgcgaagcc acctttgttt ttcgttgatg
     2161 cggaataaat ggcccgggcc cgttcatcta tgacttttaa tgtagcaaga attgatatgg
     2221 gatatttgtc acttgtgatc ggttcagcgt taagaatgtc ttccggtaaa atagaggatg
     2281 gaaagaaatg ctggccttcc tgcaggaatt tagggatata atcaagatga tcgttgtata
     2341 tgtccttata gctgctttta ctggtattga tttcgttctg gactttttta ggcagtgcag
     2401 gaaggatatc cataattatc cgcctgtaat catcctcatt cctttcaaag ttgtaagcct
     2461 ctgccatatc tgcgcgtaat tctttttccg tagcatggct cactccaaat ccagaccaaa
     2521 gttgcattga aacatccttt ttcagatcag gatcccaata agttttcctt ccttcataaa
     2581 ccgcattttc ccctttgaag tttcctcccg gatgtttcag cttgtaagct ggatgattgt
     2641 tgaaatagca ccaatcaccg atcttaaccg ggcgatcgtt ttccgctcca agttttcttt
     2701 ccttatttgc atcgaaaacg aattgtttaa aataggtctc cgatgtgtat tgcgaaatta
     2761 ccaggggggc tttgctgtca gggctggcat tgtacggggg catgtctgaa aacacttcat
     2821 cgaaacgttc gtcccccagt gcctgcctga gattgtcata ataaatagca atgactgccg
     2881 aagcacagtc ggcaattgta agcccgttaa accaggcttt cagcgcttcg gaagccttga
     2941 ccccgggttt gatgcggaag taatagatct tatcggtagt cagctgccag taattctcat
     3001 tggcataacc atagtcaaat tgtgactgcc agttatcggc atagggttta aggtttgcat
     3061 tcttaacggg atcgttttta taatcttcaa cattttcttt ataaggagct gcataatgca
     3121 cccctgtcgt tgctccccaa tcgtaattca ggtctttcag ctttccgaca aggtctgcgg
     3181 gagcattaag tgttttgctt acgttccccg cgtaggttgc tgaagaaggt atcttcagga
     3241 cctgcccgct ttttaattta taattggcgt tgttttttgc aaagtcgtca gagtccgtgg
     3301 gaatgagatt atttaatttt cgaagttcat agacggttgt gttgaagcgt ttcgcgatag
     3361 cattgagatt gtcattttcg tcggcgacac tgtatcctcc ggtcttagta ttgaagctcc
     3421 cgtttgcacc cgccgacgct cccctgtcgg gtgatttgtc atccggtttg cgatgaatag
     3481 cattatcgct gcctgcctct tgcttcgtgt gtaccagctc atgcgcgagc aattcctttc
     3541 cttccttcga ctgcgtgttg taatgcccat ccctgaaata aatgtcctgc ccatgtgtga
     3601 aagctcttgc gttaatgctt tcgctcatag tatgtgcgtt tgcaccggtg tgaatcctga
     3661 cgttgctaaa gtcagttccc atagttgttt ccatttccat tttcgcggaa tcatcaaggg
     3721 gccgcccgct gcctttcgac gtgctcagcg atgaccggat cggatctgct gaagggagaa
     3781 ggtcttcctg tccttttgcc tggattgcga aagtggatgt taccggggtg ctgaaatcga
     3841 gtcgctgacc actggctact ttttgggcaa ctgcatccgc ttctttttcg gaagcgtttt
     3901 cgggattgct gacggtcaaa ggagatgatc ccggcctgcc ctttgcgtct gcattctttt
     3961 tccgttgagc ttcctcttcg tcaagcatcc ttcggtgaag atgaaaccgc tcgttgctgt
     4021 aatgatcgta gatcataatg cgcctttgca agggaatttc cgacaaatcg cacacatttc
     4081 caagaatgaa tttgagaaga catggtgctg aaaacgatta gggaaggatt gatcgtgtct
     4141 tcgctcagtc atggaatctc cacgcggatt cacactaatg caaaggagac ggaaaaaatt
     4201 tgcaaccggc ttaacgctca aacctcccgg agacttccct catctccctt tttccccacg
     4261 atgaaatcac cgatgataag ataatccatg tcggcgcgtt cgaagctgtg caccgcatct
     4321 tcgggtgaag cgacgatcgg ttcgcctttg atgttgaacg aagtgttcag caggacggaa
     4381 taaccggttt cttttttgaa cgcttcgagc agcttccgca tttcgggatt ttccgtcgca
     4441 ttcacggtct ggatgcgcac gctgttatcg acgtgcgtga tggccggcat tttcccgagg
     4501 tactccgggc gcacgttgta cacgaaaagc atatatggat tcgggaagct gctgtcgaaa
     4561 tattttccgc aatcttcttc gaggacgatc gctgcaaatg gccggaacgg ctcgcggaac
     4621 ttcacgcgcg cattcaccac ggctttcgtg ttttccccga gcggcgaggt gatgatgctg
     4681 cggttgccca gggcccgcgg accgaattcc atcctgccct ggaaccagcc gaggatcttg
     4741 tttttcgcca gctgcctggc ggcgaatgcg cagtagtcat ccgtgcgcac gtacggaagt
     4801 cctgattttt tcagcgctgc ttcatagtct tccgtcgcgt agcccggacc caggaacgcc
     4861 gtttccatga tccatttccg ttcccgtttc agcacgccgt ggttgagcat gagcgctgcg
     4921 cccagcgcgg tgccggaatc accggctgca ggttgtatga aaatattttt gaaaagtcct
     4981 tccttcgcga tgagcccgtt cgtcacgctg ttcagcgcca cgccgccggc catgcagaga
     5041 ttttccgagc cggtattttt tttcagcgtg cgcaccatgt gcaggatcac gtcctcgacg
     5101 atatgctgcg ccgccgccgc aacatccagc tcgtgcccgc tccagtcttt tccttcgctc
     5161 ttcgatgcgc cgaacaggtc cgtgaatttt ttcgtcacgc ctgttttccg cgtgtagtga
     5221 tagcagaaat acgacaggtc gagcctgaac ccgccgtcgc cggtaagttt tacaaggctg
     5281 cgcattttcc ggtaaacgtc cgtatccgct tttccgtacg aagcaagtcc catcagcttc
     5341 ccgggtcctt cgatcagcct gaaaccgagg tgacgcgaaa tggcctggta aaacgcaccg
     5401 agcgaatacg gggtattcac accgccgata cggcgtatgt cgttcccttt tccgtgtgcg
     5461 aacatgctcg ttgtccattc gcccgcaccg tcgatcgtga ggatggcggc atcgtcgtac
     5521 ggggtgggga agaaggcgct cgcggcgtgg cagaaatgat gttcgagcag gtggaaacgg
     5581 aatttcgcct cgcgttccga aggaaaaagt tttttcagcg tgcccggaag acgtttcatg
     5641 tctttcaggt aattgagcat gccgaggttt tcctccacag aaaagctgcg ctgctcacgc
     5701 agcagctgcg gaaccttgtt ccagaatttc aggaggaaaa cgggaacttt cgaatacgag
     5761 atcgacggtt tccagaagaa cgcgacgtga tcgatgtcgg cgagcgtgag gttcgcgtgt
     5821 tccagcgcag cacggatcga cgcttcgggg attccgccgt aatgtttttt ccggttgagc
     5881 cgctcttctt ccacggcgaa aacgacttca tcatccacca tcagcgctgc tgcggaatca
     5941 tgaccataac aattgaatcc gagaatgttc ataacggtta gcgggttcct tttcctttga
     6001 gcgcaatatc gagattgttt ttggccagct ggaaatcggg tttgatcttc agcgcttttt
     6061 tccctgcgtc gatggcgata tcccattcgt gcagcgcgtt cgcgtaagcg cagatgttgt
     6121 tccatcccgt ggcgtcgccc gggtttattt ttacggcctg ctgtcctgcc gcgagggcgc
     6181 gcccgtaatt tcctgccgcg taccactgca tgctgaggct gacatagccg gtgtaggaag
     6241 gatttttccg gaggctgtct tcgagctgcg tgaaatgcgc ctgctgcgat aaggcaaaag
     6301 caaggttgtt tttggaaagc gcatccgttg gggcaagctg cacggctttc tgtcccgcgg
     6361 caatggcttc gtcgaattcg cccattttgt tgtaggcggc gcacgcattg ttccatgcat
     6421 tggcataggc aggattgatc gcagccgctt tcagcgcggc accggcgctc tgcgcgtatt
     6481 gtccgtcgtt gtaataagcg aggctgagca ggacgtaatt atcggcggtg ggatttttct
     6541 gcgctgcatc gatcgccatg tcgagccggg tgtgcacagt gccgttcgcg aataaactga
     6601 gcaggttgtt gatgccttcg tgctgcggac tgaccgtttt tcctttttcg aggatcgctt
     6661 tcgcttcgtc catccgttca tgctgcacga ggaaatctcc atagtagtaa taggattccg
     6721 ggttgagcga atcgagcgag agcgcgtaac ggaaattttt ctctgcggct tccgtgtttt
     6781 tacgcccgtt ttccgcgatg cccatgttga tgtacaggta ggaataatac gggtaggtct
     6841 ttagcccgcg gttaaagcaa tcgatcgcgc cgtcgtaatc gcttttcgcc atcagcgaaa
     6901 gtccgtaatt catccatccg cgcccgttgt ccggactttt ttccgtcaca tccttccaca
     6961 atgtttcacc gctgctccac acgtggttgc gttcatgcgt gcctgcggcg tgcgcgagca
     7021 ggagcacgac tgcgaagacg gagaggcctg cgcgcacggg aatgtttttt ccctgctgtt
     7081 ccgttttttc caggatgagc atggcgatgc tcgccacggc aaacacaagc ccgatgaacg
     7141 ggaagaaaac gcggtgatca ttcagcactt cggcgaaaga aaaaatgctc gacgtcggtg
     7201 cgagcgcaat gaaaaaccag gcgatgccga atgaaatccc ttttgtttcg ttcttcccgg
     7261 aaagctgcac cgccgtgatg aataacgcga tgatgatgcc ggccccggcc aggatgcgca
     7321 ggtcgaacat gttcgtgatc agcgtccagt cggtatccgc gctcagctgg ttgggaataa
     7381 agaacgtgta gaggtaatgc gcgatcaccc acgcctgtgt ctgcaggtaa accagcgggg
     7441 cgcctccgcc tgatgtccag tgcggcgg
//
LOCUS       DASTSF010000332         5077 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_423884,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000332 DASTSF010000000
VERSION     DASTSF010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5077
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_423884"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2681
                     /locus_tag="VFU15_09470"
     CDS             <1..2681
                     /locus_tag="VFU15_09470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718052.1"
                     /translation="VTNSDVWFDQVSQPDSVTVFAKVTNLGRAVNDSFLVKMQRTFPN
                     GDTVSYIRLVRGPKFRDTLSFTIPVDQQRGVGLNKISISLDYMSWITEMREDNNSTQP
                     DVDLLIHGSAIIPVYPYDFAVIPSDTITLKACTVNPLEPAKTYRFELDTTDLFNSPFK
                     QSYTVNSVGGVVQWKPNLTLTDSLVIFWRVSPDSLTANDVFRWRQSSFQYINGQTGWG
                     QDHIFQFTNDNYQYVRLNRQNRDFEFVNDIINLNVQDGIFPYALQWNDIFYKLNGATE
                     HIFSCVLGFGGNGVTIAVIDPVSGIPWNYASNTVGYMPQGYLDCVGGMQQLNAFDFFD
                     NDTTNRGYIRDFLDSIPNGYRVLIYSQNYHSDTQYEPGLVSALQSIGSSTVAAGTIQD
                     TIPFLIWGTKGGAPGTATELVGTDLHSVLHLQDTLITNWYKGYVQSPLIGPSMQWGSF
                     HWKEHPSETPDNDSAYVDLYGYDAAGNATFIHRFPKDTTDYYNLNALVPAAQYPYIRL
                     VARERDDTTHTPPQIDRWHVLYTPFPDAAVNPPLAFSLHSDTLQEGDNVKLVVGIQNV
                     TPWPFNDSLLLTYWVVDRNNMKHPLPQKLKAPPFNGYHWFPDTLTFSSTGYAGVNQLW
                     VEVNPVGDPNSQLEQYHFNNVMMVPFTVSTDKTNPLLDVTFDGVHIMDNDIVSAKPNI
                     LITLKDENQFLALNDTSDFNVFLKTPSQTVAQLIHWGPDMIFTPAVLPNNSCKILFDP
                     QCTEDGTYELIVQAKDRSNNESGVYDYRITFEVINKPSVTNVLNYPNPFSTSTRFVFT
                     LTGSEVPEVFTIQIMTITGKVVKEINRDELGDIHIGRNVTDYYWDGTDMYGDPLANGV
                     YLYRVITRLNGEAVDQRDSGADQYINHGYGKMYLMR"
     gene            complement(2721..>5077)
                     /locus_tag="VFU15_09475"
     CDS             complement(2721..>5077)
                     /locus_tag="VFU15_09475"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEU4718053.1"
                     /translation="PISFYTTQSNPCASSCNGSISVTVASSPGTYEYSDDGGTTWQSG
                     SAFTGLCAGTYSIAVRNASIPGCTASHVVTLNSQAAITATPAVTNPTCFGSCNASIHV
                     TASPAGSYQYSKNGGVNWQSSGTFSALCAGTYTVVVKNSSGCLSASVPVTVTDPPQLA
                     AAVSSSAGPLCFGDTTGHASISASGGTGTLSYNWSPGNPHGDGTASATGLGAGTWSCQ
                     VTDANGCTTTNSVTLTQPPQVSVSPASHSDVQCNGDTDGVVVINASGGTGTFTYSWSN
                     GGSTNSLSNLGAGSYSCTITDANACTSTFTVSITQPPLLVSSVVSFSNVSCNGNTDGA
                     ASVAASGGTGILLYDWTPGNPPGDGTGNVNALSPGTWSCMITDGNGCTSSQTVLITEP
                     PAISVVVESTAGVTCNGGDDAFAGVTVFNGQGMLSFDWSPGNPAGDGTSNVTGLSAGT
                     YTCSVTDTAGCTGSVSLTITQPAAVIVSANAFPALCHGSCDGAIQGSASPAGTYLFSI
                     DNGQSWLPGGSFSGICAGTYSVIAEDAAGCTDTVTGISVQEPSIVTAVTNVVPTSCGL
                     CNGAASVTASGGVAPYSYDWINGGQGPDVCAGTYTVVISDSHACMDTVVLFVDASVPL
                     LVDSTSAAGTPPLQSLGTATVYPSGNGPFVYAWDNAAGNQSTQTATGLAAGSYCVVVT
                     DSSGCSDTACVNVPVITGIADHSPGGFSVYPNPFSDHISVEGKGQVHLSVVNMTGETV
                     ADLGVVVLNGEKEISLPAGIPPGMYFIRIDDGKNNFMIRLAHAD"
BASE COUNT         1182 a         1484 c         1336 g         1075 t
ORIGIN      
        1 aggtcacgaa ttccgatgta tggttcgacc aggtttcaca gccggacagc gtcaccgtct
       61 tcgccaaagt gacgaacctg ggtcgcgcgg tgaatgattc tttcctcgtg aaaatgcagc
      121 gcacgttccc caacggcgac acggtgagct atatccgcct ggtgcgcgga cctaaattcc
      181 gcgatacgct ttcgttcacc attcccgtcg accagcagcg cggcgtggga ctgaacaaga
      241 tcagcatctc gctcgattac atgagctgga tcacggaaat gcgcgaggac aataattcca
      301 cgcagcccga cgtcgacctg ctcattcacg gaagcgcgat catcccggtg tacccgtatg
      361 atttcgccgt tatcccgtcg gacacgatca cgctgaaagc ctgcacagtg aacccgctgg
      421 aaccggcgaa aacctatcgc ttcgagctcg acacgaccga cctgttcaac agcccgttca
      481 agcaatcgta cacggtcaat tccgttggcg gcgtcgtgca gtggaaaccg aatctcacat
      541 tgaccgacag cctggtgatc ttctggcgcg tgagtcctga ttcgctgacg gcaaacgatg
      601 ttttccgctg gagacaaagc tcgttccagt acatcaacgg gcaaaccggc tggggacagg
      661 atcatatttt ccagttcacg aacgacaact accagtacgt tcgcctcaac cggcagaacc
      721 gcgatttcga attcgtcaac gacatcatca acctgaacgt gcaggatgga atttttccgt
      781 acgcgctgca gtggaacgat attttctaca agctgaacgg ggcgacggaa catatttttt
      841 cctgcgtgct cggcttcggc ggcaacggcg tgacgatcgc ggtaatcgat cctgtgagcg
      901 ggatcccgtg gaactatgcc agcaacaccg tcggttacat gccgcagggt tacctcgact
      961 gcgtgggcgg catgcagcag ctgaatgcat tcgacttttt cgacaatgac acgacgaacc
     1021 gcggttacat ccgcgatttt ctcgacagca tcccgaacgg ttaccgtgtg ctcatctaca
     1081 gccagaatta tcattccgat acacagtatg aacccgggct tgtttctgcg ctgcaatcca
     1141 tcggaagctc caccgttgct gcgggaacga tacaggacac gatccctttc ctcatctggg
     1201 gaacgaaagg cggcgctccg ggaactgcaa cggaacttgt tggtacagat cttcattcgg
     1261 tgctgcacct gcaggacacg ctcatcacga actggtacaa aggttatgta cagtcgccgc
     1321 tcattggtcc gtcgatgcaa tggggttcct tccactggaa agagcatccg tcggaaacac
     1381 cggacaacga cagcgcgtat gtcgatcttt acggttatga tgcagcgggc aatgcgacat
     1441 tcattcaccg ttttccgaaa gacacgaccg attattacaa cctgaatgcg cttgttcccg
     1501 ctgcgcaata tccttatatc cgcctcgtgg cgcgcgaacg tgatgacacc acgcacacgc
     1561 ctccgcagat cgatcgctgg cacgtgcttt atacgccgtt ccccgatgct gccgtaaatc
     1621 cgccgctcgc attcagcctg cacagcgata cgctgcagga aggcgataac gtgaagctcg
     1681 tggtcggaat tcagaacgta acgccgtggc cgttcaacga cagcctgttg ctcacttact
     1741 gggtggtcga ccgcaacaac atgaagcatc cgctgccgca gaagctgaaa gcgccgccgt
     1801 tcaacggtta tcactggttc cccgatacgc ttacgttcag ctcgacaggc tacgcaggcg
     1861 tcaaccagct ctgggtggaa gtgaatcctg tcggtgatcc caattcgcag ctggagcaat
     1921 atcatttcaa caacgtgatg atggtgccgt tcaccgtgag cacggataaa accaacccgc
     1981 tgctcgacgt aacgttcgac ggcgtgcaca tcatggacaa cgacatcgtt tccgccaagc
     2041 cgaacatcct catcacgctg aaagacgaga accagttcct tgcgctgaac gacacttccg
     2101 atttcaacgt gttcctcaaa acgccatcgc aaaccgtggc gcagctcatt cactggggac
     2161 ccgatatgat cttcacgccc gccgttcttc cgaacaacag ctgcaagatc cttttcgatc
     2221 cgcaatgcac agaggacgga acgtacgagc ttatcgtgca ggcgaaagac cgttcgaaca
     2281 atgaaagcgg cgtttacgat taccgcatca cgttcgaagt gatcaacaaa ccttccgtga
     2341 cgaacgtgct taattacccg aatcccttca gcacgtccac gcgttttgtg ttcacactga
     2401 cgggctccga agttcccgaa gtgttcacga tccagatcat gaccatcacc gggaaagtgg
     2461 tgaaagagat caaccgcgac gagctcggcg acattcacat cgggcgcaac gtaacggatt
     2521 attactggga cggaaccgac atgtacggcg acccgctcgc gaacggcgtt tacctctacc
     2581 gtgtcatcac gcgtctgaac ggtgaagccg tcgaccagcg cgacagcggc gccgaccagt
     2641 acatcaatca cgggtacggg aaaatgtacc tgatgcggtg aaaagatttc acttcgtttt
     2701 atcctgagaa aatttgcgca tcagtctgcg tgcgccagcc ggatcatgaa attatttttt
     2761 ccgtcgtcga tacggatgaa gtacatgccc ggtggaattc cggccggcaa tgaaatttct
     2821 ttttcgccat tgaggacaac cacgccgaga tccgcaaccg tttcacccgt catgttgacg
     2881 accgaaagat gaacctggcc ttttccttca acggagatgt gatcggagaa cgggttcgga
     2941 taaaccgaaa aaccacccgg agaatgatct gcgattcccg tgatcaccgg cacattcacg
     3001 caggctgtgt cggaacaacc gctgctatcc gtaacaacga cgcaataact tcccgctgcg
     3061 agaccggttg ctgtctgtgt tgattgattt cccgcagcat tatcccaggc ataaacgaag
     3121 ggtccgttcc ccgaaggata aaccgtcgcc gttcccagcg actgcagggg aggcgttccc
     3181 gcagcagatg tggaatcaac caggagcgga acggaagcat caacaaaaag gacaacagtg
     3241 tccatgcacg catgcgaatc gcttatgacc actgtatatg ttccggcaca aacgtccgga
     3301 ccttgcccgc cgttgatcca gtcgtacgaa taaggcgcaa cgccgcctga cgcagtaacg
     3361 gaagcggccc cgttgcacaa tccgcagctc gtgggaacca cattcgtaac cgcggttact
     3421 attgaaggct cctgcacgct tattcccgtc acagtgtccg tacatccggc cgcgtcttcg
     3481 gcgatcaccg aataagttcc ggcgcaaatt ccactgaacg aaccgccggg cagccagctt
     3541 tggccgttat cgatcgagaa gaggtatgtt cctgcaggac ttgccgagcc ctggatcgcg
     3601 ccatcgcacg aaccgtggca caaagcagga aatgcgttcg cggaaacgat caccgctgca
     3661 ggttgcgtta ttgtcagcga aacagaaccg gtgcaacctg ccgtatcggt aaccgagcag
     3721 gtgtacgttc ctgcggaaag tcccgtgacg ttgctggtcc cgtctcccgc ggggtttccc
     3781 ggcgaccagt cgaaagacag cattccctgc ccgttgaaaa ccgtaacgcc ggcaaatgcg
     3841 tcatcaccgc cgttgcaggt cacgcctgcg gtcgattcca ccaccacgct gatcgcgggc
     3901 ggttcggtta taagtacagt ttgcgaagaa gtacaaccgt tcccgtcagt gatcatgcac
     3961 gaccatgtac cgggactgag cgcatttaca tttcctgttc cgtcgcctgg aggatttccc
     4021 ggtgtccagt cgtacagtaa aattcctgtc cccccgctcg ccgcaacgga tgcagcgccg
     4081 tccgtatttc cattacagga aacattgctg aaggaaacca ccgaagaaac gagcagcggg
     4141 ggttgcgtga tgctgacggt aaacgtcgat gtgcatgcgt tcgcgtctgt aattgtacag
     4201 gaataggaac ctgcgccgag attgctgaga gaattcgtgc ttcctccgtt gctccaggaa
     4261 taggtgaacg ttcccgttcc gccactcgca ttgatcacaa caacaccatc agtgtctccg
     4321 ttgcattgca cgtcagaatg cgacgccggc gaaacactga cctgcggcgg ttgcgtcaag
     4381 gtcactgagt tcgtggtcgt acaaccgttc gcatccgtca cctggcagct ccacgttccg
     4441 gcgccaagac ccgttgctga agccgttcca tctccgtgcg gatttcccgg cgaccaattg
     4501 tacgacaacg ttcccgttcc accgctggcg cttatcgaag catggcctgt cgtgtcgccg
     4561 aagcaaagtg gtccggcgga tgaagagaca gccgcggcca gttgcggcgg atccgtgacc
     4621 gtcaccggga cagaagcact gagacacccg gaagaatttt tcaccaccac tgtgtatgtt
     4681 ccggcgcata acgcgctgaa tgtgcccgaa gattgccagt tcaccccgcc gttcttgctg
     4741 tattgatacg acccggcagg agatgctgta acatgaatcg aggcgttgca gcttccgaag
     4801 caggtcgggt ttgtaacggc cggtgttgcg gtgatcgcag cctgcgaatt caatgtcact
     4861 acgtgcgacg cagtacaccc cggtattgaa gcattgcgca cggcgatcga atacgttccg
     4921 gcacaaagcc cggtgaatgc agaaccgctt tgccacgtcg tgcctccatc atcactgtat
     4981 tcgtaggttc ccggcgacga tgccacagtt actgaaatgg aaccgttgca cgatgatgcg
     5041 cagggattgc tctgcgttgt gtagaaagag atgggag
//
LOCUS       DASTSF010000333         6765 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1083249,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000333 DASTSF010000000
VERSION     DASTSF010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 6765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6765
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1083249"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..953)
                     /locus_tag="VFU15_09480"
     CDS             complement(<1..953)
                     /locus_tag="VFU15_09480"
                     /inference="COORDINATES: protein motif:HMM:NF024991.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU4718054.1"
                     /translation="MNFADEIVKQLPFSATEKQRLAADEMAKFLTDPDPHRMFVLRGY
                     AGTGKTTLLSALVRALPSLKMKSVLLAPTGRAAKVLAGYSGKQAWTIHKKIYRPSGSG
                     EGAYFSLQGNPYRNTLFIVDEASMIGAGNPEANLFPSSVLEDLFHFVYSGENCRLLFT
                     GDTAQLPPVGMNASPALDPGFLKNTFRVPVTMTEMNEVVRQEMESGVLLNATRLRIDI
                     AEGKKEFPQFITEGYNDVVRVQGSELVELIEECYRKYGPDETIVICRSNKRANIYNQQ
                     IRVRIRWQEDEIAAGDHVMIVKNNYTWLPEESKAGFIANGDT"
     gene            1122..1994
                     /locus_tag="VFU15_09485"
     CDS             1122..1994
                     /locus_tag="VFU15_09485"
                     /inference="COORDINATES: protein motif:HMM:NF024271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3822 family protein"
                     /protein_id="HEU4718055.1"
                     /translation="MTELKRTISPTVEFTDAAFDPGQSEKFCLVAEVSPLSMELAVLD
                     NITNDFLAFAQYVFRKPAPGKMAAELGNIRAGHPWLGNGFKRTDVMVATEKFTLVPSA
                     FFDSSAIHEMLAFNHPVNENEIVLNDILRNADARIVYAVENSVEKELRKISGAVRIRH
                     HFTPLIESFISANKNRMERKALAHVHGQSLDIVIAEKGKLLLANTFTFRAPEDFIYYL
                     LFCCEQLKMNPETMELEIAGELETDSAVSGLARKYVRNVHFSARPPEMRFTKGFEQFP
                     PHYFRNLFSLHYFS"
     gene            1991..2533
                     /locus_tag="VFU15_09490"
     CDS             1991..2533
                     /locus_tag="VFU15_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010519724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HEU4718056.1"
                     /translation="MIRIIGGELKGRRISAPASLPVRPTTDFAKEGLFNVLNNRFGFE
                     EISALDLFSGTGNISFELASRGCRDITSVDGDIRCVNYISKTARELHFSQIHTVKADS
                     YVFLARARRTWDLIFADPPYAQKQAAEIPGLVFSHALLNTNGILIVEHEERFVFPDTS
                     KLLERRRYGKVNFSIFGETN"
     gene            2535..2999
                     /gene="coaD"
                     /locus_tag="VFU15_09495"
     CDS             2535..2999
                     /gene="coaD"
                     /locus_tag="VFU15_09495"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020571904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEU4718057.1"
                     /translation="MPHIAVFPGSFDPFTKGHESIVHRCLPLFDRIIVAIGVNSHKTY
                     FFPLEKRKEWIRKTFEGEANVSVETFTGLTIDFCRKMNAKYILRGLRTTTDLEFEKAI
                     AQMNKSLAEEIETLFILPTPELSAINSTIIRDIVRNGGDASPFVPAAVDLTR"
     gene            3030..4460
                     /locus_tag="VFU15_09500"
     CDS             3030..4460
                     /locus_tag="VFU15_09500"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-like domain-containing protein"
                     /protein_id="HEU4718058.1"
                     /translation="MKFTLPVFCVFLTLSSFAAVKDSTCTIYGTAPGREGNVIQVLEY
                     DDYITYTEEKLAETVVGDSGKFSLSFDIGEVTNVFLRCKNVHGFLFAEPGRNIEVMLP
                     ERDSLLQLNPNVDYKVPVNVTIDLDDSTDMNFLASDYNWRFNTWWDTMFVYFVTKQSM
                     KPMNDFHAHMDSLYARVKNPYFKPWMDYSMASIEDATFNNQVTLAKKYLDGKPVYYHN
                     SEYMEFFNSFFKDYLYRWSMRKEGEGISFAINMRGSYDSLMGVMKHLPWMENDSIREL
                     VMLKGLFELYNNPAFDPGNILSIAQQASVRSKISESRRIARNILRYYTKLKNGTAAPH
                     FLVITRKDELVDPLDQYKGKFIYLFFYDTKNPASMAEYRYMSELQKRYRKVVFVSVSV
                     DDDTAAWKKFLKTNPKYNWPNYHYDFRQKTRDDYNLYSTPSGFLIDPDGNLYASPADN
                     PSGDLEFMLYKICYPKKPALQRPGDR"
     gene            complement(4457..5077)
                     /locus_tag="VFU15_09505"
     CDS             complement(4457..5077)
                     /locus_tag="VFU15_09505"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEU4718059.1"
                     /translation="MYKVFINNHPVVFTDDSFAAEVAAGSLFVRDNSEKVLDEIIQLA
                     NRYKDFFNVIYFVTDDPEKMFHTLVSRCRVIEAAGGVVKDPQGETLMIFRRDKWDLPK
                     GKIDPGETPEAAALREVEEECGIRGHRIVKKLSPTYHAYMQNETFVLKKTYWYEMRVD
                     EKLLLTPQAEEGITEVKWMDRAGERDALKNTFHSVEDVMAEVNKKG"
     gene            5087..5728
                     /gene="pyrE"
                     /locus_tag="VFU15_09510"
     CDS             5087..5728
                     /gene="pyrE"
                     /locus_tag="VFU15_09510"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEU4718060.1"
                     /translation="MITNAEIAAKVAQFLLQIKAVKLQPKQPFTWASGWKSPIYCDNR
                     KTLSYPKIRTFIRQQFVQVIGQNFSQPDVIAGVATGAIAQGVLVAEEMGLPFVYVRPE
                     PKKHGLGNLIEGEIKPGQSAVVIEDLISTGGSSLRAVDALREAGCEVKGMAAIFTYGF
                     STAADAFKKANVNLVTLSDYSSLIRQALKDDFVSEDELETLEAWRNNPASWGN"
     gene            5833..6234
                     /locus_tag="VFU15_09515"
     CDS             5833..6234
                     /locus_tag="VFU15_09515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718061.1"
                     /translation="MSRIESDVVETNKSQEHIFNFLTDFNNFQKLMPPQVTDWQSTAD
                     TCSFTINGMAKVGMRIESKTPHEKIHIVSEGGKLPFPFTLDVLVTSTGPSSCKGQLIF
                     EGDIPIFIRPMVEGPLRNFFNLLAQKMKDIA"
     gene            6416..>6765
                     /locus_tag="VFU15_09520"
     CDS             6416..>6765
                     /locus_tag="VFU15_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009185445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEU4718062.1"
                     /translation="MPKGFFNVPAPHNEPVKSYAPGSPERAELKKMIAQLRSSEIDIP
                     MHIGGKEVRSDVKVRLAPPHDHKHTLGHFHKSSKEHVKLAIDAALAARRQWQDLSWEH
                     RASIFLKAAELIAGP"
BASE COUNT         1611 a         2012 c         1626 g         1516 t
ORIGIN      
        1 gtgtcgccgt tcgcgatgaa accggctttc gattcttccg gcagccacgt gtaattattc
       61 ttcacgatca tcacgtggtc gccggcggca atttcgtctt cctgccagcg tatgcgcacg
      121 cggatctgct ggttgtaaat attcgcacgt ttattcgaac ggcagatcac gatcgtttcg
      181 tcgggcccgt atttccggta gcattcttcg atcagttcca cgagctcgct gccctgcacg
      241 cgcaccacgt cgttgtatcc ttccgtgatg aactgcggaa attctttttt cccttccgca
      301 atgtcgatcc ggagacgcgt tgcgttcagc agcacgcctg attccatttc ctggcggacc
      361 acctcgttca tttcggtcat cgtcaccggt acgcgaaatg tatttttgag aaagcccgga
      421 tcgagcgcag ggctcgcgtt cattcccacc gggggaagct gcgcggtgtc gcctgtaaaa
      481 agcaggcggc aattttctcc gctgtagacg aaatggaaca ggtcttccag cacgctgctc
      541 ggaaaaaggt tcgcttccgg atttcctgca ccgatcatag acgcttcgtc gacaatgaaa
      601 agcgtgttcc ggtagggatt tccctgcaat gaaaaatagg cgccttcccc ggagccggac
      661 gggcggtaaa tttttttgtg aatggtccac gcctgcttcc cggaatagcc cgccagcact
      721 ttcgccgcac ggccggtcgg tgcgagcagg acgcttttca ttttcagtga aggaagcgca
      781 cggaccagcg cgctgagcaa cgtggttttt cccgtgcccg catacccgcg cagcacgaac
      841 atgcggtgcg gatcaggatc cgtaaggaat ttcgccatct catccgctgc gagccgttgc
      901 ttttctgttg cactgaacgg aagctgcttc actatttcat ctgcgaaatt cacggcgcta
      961 aagtagtgaa ctgcaaactg caaaaaacga acagcgaacg atagcccctt ttcaactttc
     1021 caacctttca actttttgac ctttcaactt accaacctcc cgacctttcc aactataaac
     1081 ccctcccgcc acttaatcac ttaagtagta ccttcgcagc aatgaccgaa ttgaagcgca
     1141 ccatttctcc aaccgttgaa ttcaccgacg ccgctttcga tccggggcag tccgaaaaat
     1201 tctgcctcgt ggccgaagtg agcccgcttt ccatggagct ggccgtgctc gacaacatca
     1261 cgaatgattt tctcgcgttc gcacaatacg ttttccgtaa acccgctccg ggaaaaatgg
     1321 cagcggagct gggcaatatc cgcgccgggc atccgtggct gggcaacggc ttcaagcgaa
     1381 cagacgtgat ggtcgccacg gaaaaattca cgctcgtgcc ttcggcgttc ttcgacagca
     1441 gcgccattca tgagatgctt gcgttcaacc acccggtgaa tgagaacgag atcgtgctga
     1501 acgacattct ccgcaatgca gacgcacgca tcgtctacgc ggttgaaaat tcagtggaga
     1561 aagagctgcg aaaaatttca ggggccgtac gcatccgtca tcatttcacg ccgctgatcg
     1621 aatctttcat ctccgcgaac aagaaccgca tggaacgcaa agcgcttgcg catgttcacg
     1681 gccagtcgct tgacatcgtt atcgctgaaa aaggaaagct cctgctggcg aacacgttca
     1741 cgttccgcgc cccggaagat tttatctatt accttctttt ctgctgcgaa cagctgaaga
     1801 tgaacccgga aaccatggag ctggagatcg ccggggagct ggaaacagat tccgcggtat
     1861 ccgggctcgc caggaagtac gtgcgcaacg ttcatttttc cgcacgtccg cccgagatgc
     1921 gtttcacgaa gggattcgaa cagttcccgc cgcattattt ccggaacctt ttttcactac
     1981 actatttttc atgatccgga tcatcggcgg tgagctgaaa ggaagacgca tcagcgcgcc
     2041 tgcgagcctg cccgtgcgcc cgacaaccga tttcgcgaaa gaaggtctct tcaacgtgct
     2101 caacaaccgt ttcggttttg aagaaatttc cgcgctcgat cttttcagcg gaaccgggaa
     2161 catcagcttc gaactggcgt cgagaggctg ccgcgacatc acttccgtcg acggcgatat
     2221 ccgctgcgtg aattacattt cgaaaaccgc acgcgagctt catttttcac agatccatac
     2281 cgtgaaggcc gacagctacg tgttccttgc gcgcgcgcgg cgcacgtggg acctcatttt
     2341 cgccgatccg ccctatgcac agaaacaggc ggcagaaatc ccgggactcg tgttttccca
     2401 cgcactgctg aacacgaacg ggatcctcat cgtggagcac gaagaacgtt tcgtgtttcc
     2461 tgacacgtcg aagctgctgg aaagaaggcg ctacggaaaa gtgaacttca gtatcttcgg
     2521 ggaaacaaac tgacatgccg cacatcgctg ttttccccgg ttccttcgat ccgttcacca
     2581 aaggacacga atcgatcgtt cacaggtgtc tcccgctttt cgacaggatc atcgtggcca
     2641 tcggcgtgaa cagccacaaa acctatttct tcccgctcga aaaaagaaag gagtggatcc
     2701 ggaaaacgtt cgagggagaa gcgaacgtat ccgtggaaac tttcaccggc cttactatcg
     2761 atttctgccg gaaaatgaat gcgaaataca tcctgcgcgg actacgcacc accaccgacc
     2821 ttgaatttga aaaagcgatc gcgcagatga acaagtcact ggccgaggag atcgaaacgc
     2881 tttttatcct gcccacaccc gagctgtccg caataaattc gacgattatt cgtgatatag
     2941 tacggaacgg cggagatgca tcccccttcg ttcccgccgc agtggacctc acccgctgac
     3001 tccggcacag gatttgaaaa aaccagcgca tgaagttcac ccttcccgtc ttttgtgttt
     3061 ttttaacgtt gtcgtccttc gctgcggtga aagattccac ctgcacgatc tatggtacag
     3121 cccccggccg tgaaggcaac gtcatccagg tgctggaata cgacgattac atcacgtaca
     3181 ccgaagagaa gctggcagag accgttgtcg gcgacagcgg aaaattctcg ctttcgttcg
     3241 atatcggcga agtgacgaac gttttcctgc gctgcaaaaa cgtgcacgga ttcctgtttg
     3301 cagagccggg ccggaacatc gaggtgatgc tgcccgaacg cgattcgctc cttcagctga
     3361 acccgaacgt cgattacaaa gtgccggtaa acgtcaccat cgacctggac gattcgaccg
     3421 acatgaattt tctcgccagc gattacaact ggaggttcaa cacctggtgg gacacgatgt
     3481 tcgtttattt cgtcacgaag caatcgatga aacccatgaa cgatttccat gcgcatatgg
     3541 acagcctgta tgcgcgggtg aaaaatccct atttcaagcc gtggatggat tacagcatgg
     3601 cctcgataga ggacgcgacg ttcaacaacc aggtcacgct cgccaaaaaa tacctggacg
     3661 ggaagccggt gtattaccac aattccgaat acatggaatt cttcaacagc ttcttcaagg
     3721 attacctcta caggtggtcg atgcgaaagg aaggcgaggg aatttcattc gccatcaaca
     3781 tgcgcggcag ctacgacagc ctgatgggcg tcatgaagca cctgccatgg atggaaaacg
     3841 attccatccg cgagctcgta atgctgaaag gcctgttcga gctgtacaac aaccccgcgt
     3901 tcgatcccgg caacattctc tccattgcgc agcaggcgtc ggtgcgcagc aaaatatcgg
     3961 aaagccggcg catcgcacgc aacatcctgc gttactatac gaagctgaag aacggaacgg
     4021 ctgcgccgca tttcctcgtc atcacgcgca aggatgagct cgtcgatccg ctcgaccagt
     4081 acaaggggaa attcatttac ctgtttttct acgacacgaa aaatccggcg tccatggcgg
     4141 aatacaggta tatgtcggag ctgcagaaac ggtacaggaa agtggttttc gtcagcgttt
     4201 ccgtggatga tgatacagcc gcctggaaaa aattcctgaa aacgaacccg aaatacaact
     4261 ggcccaatta ccattacgat ttccgccaga aaacaagaga cgattacaac ctctactcca
     4321 cgccgagcgg ttttctcatc gatcccgacg ggaacctgta cgcttcaccc gccgataatc
     4381 cttccggcga cctggagttc atgctgtaca agatctgtta cccgaaaaag cccgcgctgc
     4441 agcggcccgg cgaccgttaa ccttttttat ttacctccgc catcacgtct tccaccgaat
     4501 ggaacgtgtt cttcagcgcg tccctctcgc ctgccctgtc catccacttc acctccgtaa
     4561 tcccttcctc cgcctgcggc gtcagcagga gtttttcatc gacgcgcatt tcgtaccagt
     4621 atgttttttt cagcacgaac gtttcgttct gcatgtaggc gtgatacgtc ggtgaaagtt
     4681 ttttcacgat gcggtgcccg cggatgccgc actcttcctc cacctcgcgc agcgcagccg
     4741 cttccggcgt ttcacccggg tcgatctttc ccttcggaag atcccatttg tcgcggcgga
     4801 agatcatcag cgtttccccc tgcggatctt tcactacgcc gcccgccgct tcgatcacac
     4861 ggcagcgcga caccagcgtg tgaaacattt tttccggatc gtccgtcacg aaataaataa
     4921 cattgaaaaa atccttgtac cggttggcga gctgtatgat ctcgtcgagc actttctcgg
     4981 aattatcgcg cacgaaaagg gagccggcag caacctccgc ggcaaaagaa tcgtccgtaa
     5041 aaaccacggg atggttgttg atgaaaactt tatacatttg cgccatatga ttacgaacgc
     5101 cgagattgcc gcgaaagttg cgcaatttct tcttcagatc aaagcggtaa aactccaacc
     5161 caaacaaccg tttacctggg cgtccggatg gaaatcaccg atctactgcg acaaccgcaa
     5221 aacactttcc tacccgaaga tcaggacctt tatccgccag caattcgtgc aggtgatcgg
     5281 ccagaatttt tcccagcccg atgtcatcgc cggcgtcgcc accggcgcca tcgcacaggg
     5341 cgtgctcgtg gcggaagaaa tgggacttcc cttcgtgtac gttcgtcccg aaccgaaaaa
     5401 gcacggcctc ggaaatctca ttgaaggcga gatcaaaccg gggcagtccg ccgttgtcat
     5461 cgaggatctt atttccacgg gcggcagcag cctcagggcg gtggatgccc tgcgcgaagc
     5521 aggctgcgaa gtgaaaggaa tggcggcgat cttcacctac gggttttcca ccgcagcgga
     5581 tgcattcaaa aaagcaaatg tcaacctcgt cacgctttcc gattattcat ccctcatccg
     5641 ccaggcgctg aaagatgatt tcgtcagcga agacgagctc gaaacactcg aagcatggcg
     5701 gaataaccca gcctcctggg gaaactgaat ttcccggtaa tcaaatctga agtctcacta
     5761 ctaactactc accattcact acttcctact aactactcac tacttcctac taactacttc
     5821 ccactcacca ccatgtccag gatcgaaagc gacgtcgtcg aaaccaataa atcacaggaa
     5881 cacattttca atttcctcac cgacttcaac aatttccaga agctcatgcc gccgcaggtt
     5941 accgactggc agtcgaccgc cgacacgtgc agcttcacga tcaacgggat ggcgaaagtg
     6001 ggcatgcgca tcgaatcgaa aacaccgcac gaaaagatcc acatcgtctc cgaaggcgga
     6061 aagctcccct tccctttcac gctcgatgtg ctcgtcacct ccacaggtcc ctcttcctgc
     6121 aaaggccagc tcatcttcga aggcgacatt cccatcttca tccgccccat ggtggaaggc
     6181 ccgctccgca atttcttcaa cctgctcgcg cagaaaatga aggatatcgc gtaattcttt
     6241 tgcagcgcac ctgctgctct tttcatttcc tctcttcccg ctgtcggctc gcaggagctc
     6301 gcctccatcg cggctatgca ttcatcccgt tcaccgcagc attaactcaa aatcatcttt
     6361 acctttgcat ctcgattcca tccagtaaac agtattcaac actcacttcc gagagatgcc
     6421 caaaggattc ttcaacgtac ccgccccgca caacgaaccc gttaaaagct acgctccggg
     6481 ttcacccgaa cgcgccgaac tgaaaaaaat gatcgcgcag ctccgttcct ccgagatcga
     6541 cattcccatg cacatcggcg gaaaagaagt gcgcagcgac gtcaaagtgc gcctggcgcc
     6601 cccgcatgac cacaagcata cgctcggtca ttttcacaaa agctcgaagg agcacgtgaa
     6661 gctcgccatc gacgcggcgc tcgctgcacg caggcagtgg caggaccttt cctgggaaca
     6721 ccgcgcgtcc attttcctga aagccgccga gctcatcgcc ggacc
//
LOCUS       DASTSF010000334         5295 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1512175,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000334 DASTSF010000000
VERSION     DASTSF010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5295
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1512175"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..735)
                     /locus_tag="VFU15_09525"
     CDS             complement(<1..735)
                     /locus_tag="VFU15_09525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718063.1"
                     /translation="MRSNFKFQATYPVKIILFLSITLSCFSEKAVGQSSFRYPSCSLS
                     DSTLRPGSRIRTYSIRFAFYKDSLLAGDSTYLDSVVTFFRQHPQFFVEIGVFGSSTGS
                     SHPDTERALAIGDYLHDKGVSLDQFICGGFGIASPLYSDADISKAASKHAQDSLRALN
                     RRVEFIILDIRPEYRKTFTLSDSTVSDGSVFRTWDISFDTVTHCHLTENSRPVLDSVA
                     DFMKKHPQVSLSVVVHDDVWSWNMLGT"
     gene            complement(831..1796)
                     /locus_tag="VFU15_09530"
     CDS             complement(831..1796)
                     /locus_tag="VFU15_09530"
                     /inference="COORDINATES: protein motif:HMM:TIGR01840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HEU4718064.1"
                     /translation="MKKVPAFFFLFTTCVFSLSAADGLTELRSFGDNPGNLSCFIHVP
                     DSLASPAALVVSLHGCGENAMDCAKYTGWNDLADRYGFIVLYPQQKLVNNPNGCFDWF
                     MPDDQQRGKGEPGSIAEMIRTTEKTYRIDTTKIFVSGLSAGAAMANILLSVYPDIFSK
                     GAIFAGGPYGAAGNAPDAFSAMKGDVHKTPQEWGDDVRNADPAFHGTYPCVLIFQGDK
                     DKIVSPSNADELVKQWTNVHHTDTIADKTDTAFNGNPRVKNFIYLDSSGHAVVSFFLL
                     TDFGHAIPVDPGKGKEQGGKTGLFAKDVDFYSIYYAAEFFGLTRG"
     gene            complement(1850..3478)
                     /locus_tag="VFU15_09535"
     CDS             complement(1850..3478)
                     /locus_tag="VFU15_09535"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019039300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HEU4718065.1"
                     /translation="MDITFNKNEDTVKLLISAMEKRFGKVSEGGGKSRIEKQHEQGKL
                     TARERIDYLLDKDSPRIEIGAFAGEDMYKEYGGCPSGGVVVMIGYVKGRQCIVVANDA
                     TVKAGAWFPITGKKNLRAQEIAMENRLPIIYLVDSAGVFLPMQDEIFPDKENFGRIFR
                     NNAIMSSEGILQIAAIMGSCVAGGAYLPIMSDEAMIVDKTGTIFLAGSYLVKAAIGET
                     IDNETLGGATTHCEISGVTDYKCKDDKDCLDRIRNIMDKTGSYEKAGFDRIAPAPPKL
                     DENEILGILPDTRDKAYDMHEIIARLVDDSSFEEYKELYGQSILCGLARIDGWAVGIV
                     ANQRKVVKSKKGEMQFGGVIYSDSADKAARFIMNCNQKKIPLVFLQDVTGFMVGSRSE
                     QGGIIKDGAKLVNAMSNSVVPKFTVIIGNSYGAGNYAMCGKAYDPRLIVGWPTAQIAV
                     MGGSQAAKTLLQIQVSTLKAQGKEITPEDEKALLDKISKRYDEQTSPYYAAARLWIDA
                     IIHPLDTRKWISMGIEAADHAPVTKRYNVGVIQT"
     gene            complement(3609..>5295)
                     /locus_tag="VFU15_09540"
     CDS             complement(3609..>5295)
                     /locus_tag="VFU15_09540"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEU4718066.1"
                     /translation="YRGNGVFIGSNISSRFQLWNTSGNVYATYGNSAPDDIRGTVFIT
                     RSRAIAGTLNICSGDSTVLTSGASTSVSFQWYKDGVAIPGANSISYTTAQAGHYNVWV
                     SDECGADSLGTGVDVTINPVPVVSLGNDTAICSGGMITFDAMNAGDTYLWSDASTAQT
                     LSTGIAGTYYVNVTDPIGCSSSDTVVLNINPLPVISLGNDTAVCSGNMITFDAMNAGS
                     SYLWNDGSTTQTLMTGTAGTYFVDVTDVNGCSNSDTIMLGVNPLPVVNLGNDTAICSG
                     SMITFDAMNAGSSYLWSDSSTAQTLMTGMAGTYYVDVTDMNGCSSSDTVMLSVNAIPV
                     VNLGNDTAFCAGNMLTLDALNAGSTYMWSDASTNQTLDVTTSGNYFVDVTDTSGCSAS
                     DTIMVTVNALPTVTSSATSTNVCFDDANVSLTGTPAGGTFSGNGVTGSSFDPSAAGNG
                     NQVVTYSYTDVNGCSATSTVTITVNACTGIEEHTAASGVTLYPNPANDKLVITAAERT
                     SVMIFNATGDLVMNLDITPGTQEISVAGLPDGIYFVRSTDSKGASTQRLVITR"
BASE COUNT         1176 a         1354 c         1484 g         1281 t
ORIGIN      
        1 tgttccaagc atgttccatg accaaacgtc atcgtgcacg acaaccgaaa gtgaaacctg
       61 cgggtgcttc ttcatgaaat ccgccacgga atcgagaaca ggccggctgt tttccgtaag
      121 atgacaatgc gtgacggtgt caaatgaaat gtcccaggtt ctgaaaacgc tgccatcgct
      181 gacggtggaa tcggaaagcg taaacgtttt cctgtattca gggcgtatgt ccaggatgat
      241 gaattctacc cgccggttca gcgcacgcag cgagtcctgc gcatgtttcg aagcagcttt
      301 gctgatatcc gcgtcggaat aaagtggact tgctattccg aatcctccgc aaataaactg
      361 gtcgagagaa acgcctttgt catgcaggta atcgccgatc gccaatgcgc gttccgtgtc
      421 gggatggctg ctcccggttg aagaaccgaa tacaccgatc tcaacgaaaa attgagggtg
      481 ctgccggaaa aacgtaacga ctgaatccag gtacgtgctg tcgccggcca gcagggaatc
      541 tttgtagaat gcgaagcgaa tgctgtaagt gcggatcctg gaacccgggc gcagggtgga
      601 atcggagaga gagcacgagg gataacggaa agaagattgt ccaactgctt tctcagaaaa
      661 acaggaaagc gtgatggaga ggaagagaat tatcttcacc gggtaagttg cctggaattt
      721 gaaatttgac cgcacgcccg tttataacga ttgcacattg aaaagatacg gttgcccgga
      781 agcgggtttg tgtatttttt tatgccggcc atcgaaggac cggcaggaaa ctaaccgcgt
      841 gtcagcccga aaaattccgc tgcataatag atcgaataaa aatccacgtc cttcgcaaaa
      901 agtcctgttt tcccgccctg ctcttttcct tttccgggat cgaccgggat cgcgtgaccg
      961 aaatctgtca gcaggaaaaa gctgacgacg gcatggccgg acgaatcgag gtaaatgaaa
     1021 tttttcacgc gcggatttcc gttgaaggcg gtgtcggttt tatcggcgat cgtgtcggtg
     1081 tgatgaacgt tcgtccattg ttttacaagc tcatctgcat tcgacgggga aacgatcttg
     1141 tctttgtcgc cctggaaaat aagaacgcac gggtacgtac cgtgaaatgc aggatcagca
     1201 ttgcgcacgt cgtcgcccca ttcctgcggc gttttgtgca cgtcgccttt catcgcggag
     1261 aatgcatcgg gtgcgttgcc tgcagctccg taaggtcctc cggcaaaaat ggcgcctttg
     1321 gaaaaaatat caggataaac cgagagcagg atattcgcca tcgccgctcc cgcagaaagg
     1381 ccggagacaa aaattttcgt cgtgtcgatg cggtacgttt tttccgtggt gcgtatcatc
     1441 tccgcgatcg atcccggttc gcctttcccg cgctgctggt cgtcgggcat gaaccagtcg
     1501 aagcagccgt tgggattatt gaccagtttc tgctgcgggt agagcacgat gaaaccatag
     1561 cggtcggcga gatcgttcca gccggtatat ttcgcgcaat ccattgcatt ttcaccgcag
     1621 ccgtgcaggg aaacgacaag cgcggcggga gacgcaagac tgtcgggaac gtggatgaag
     1681 caggaaagat tgccgggatt atcgccgaaa gacctgagtt cggtaagtcc atccgctgct
     1741 gaaagcgaaa agacgcaggt cgtgaaaaga aaaaagaatg cgggtacttt tttcatctga
     1801 atgaagagtg aattttcaca ctcttctctc ttcaaacgct gttccgtttt caggtttgaa
     1861 ttacaccgac attataccgt ttcgtcacag gcgcatgatc cgcggcttca attcccatcg
     1921 agatccattt tcttgtgtcg agcgggtgga tgatggcgtc gatccacaaa cgtgccgctg
     1981 cataatacgg cgaggtctgc tcgtcgtagc gtttggaaat tttatcgagc agcgcttttt
     2041 cgtcttcggg cgtgatctct tttccctgcg ccttgagtgt gctgacctgg atctgcagca
     2101 atgttttcgc tgcctgcgat ccgcccatca cggcgatctg cgcagtaggc cagccgacga
     2161 tgaggcgcgg atcgtacgct ttcccgcaca tcgcataatt tcctgctccg taagaattgc
     2221 cgatgatgac ggtgaatttc ggaacgacgg aattgctcat cgcgttcacc agcttcgcgc
     2281 cgtctttgat gatcccgcct tgttccgaac gtgaaccgac catgaagccg gtgacgtcct
     2341 gcaggaatac gagcgggatc tttttctggt tgcagttcat gatgaagcgc gcggctttgt
     2401 cggccgaatc ggaatagatg acgccgccga actgcatttc gccttttttg cttttcacca
     2461 ctttgcgctg gttggcgaca attccaacgg cccatccgtc gatgcgcgca agtccgcaca
     2521 ggatcgattg cccgtacagc tctttgtatt cttcgaagga agaatcgtcc acgagtcgtg
     2581 caatgatctc gtgcatgtcg taggctttgt cgcgcgtgtc gggaaggatg ccgagaatct
     2641 cgttttcatc cagcttcggc ggggcgggag cgatgcggtc aaacccggct ttttcatacg
     2701 agccggtctt gtccatgatg ttgcggatgc ggtcgagaca gtctttgtcg tccttgcatt
     2761 tgtaatcggt gacgccggaa atttcacaat gcgtggtggc gccgccgagc gtttcgttgt
     2821 cgatcgtttc gccgatggcg gcttttacga gataggatcc ggcgagaaaa atggttcctg
     2881 ttttgtcgac gatcatcgct tcgtcgctca tgatcgggag gtatgcgccg cccgccacgc
     2941 aggagcccat gatcgcggcg atctgcagga ttccttccga agacatgatc gcgttattgc
     3001 ggaagatgcg tccgaaattt tccttgtcgg gaaaaatctc atcctgcatc ggcaggaaaa
     3061 ctcccgcact gtcgacgagg taaatgatcg gcaaacggtt ttccatcgcg atctcctgcg
     3121 cgcgcagatt tttcttcccg gtgatcggga accatgcgcc cgctttcacg gtggcatcat
     3181 tggcaaccac gatgcattgt cttcctttca cataaccgat catcaccacg acgccgcccg
     3241 agggacagcc gccgtattct ttgtacatgt cttcacctgc aaacgcgcct atttcgatgc
     3301 gcggcgaatc cttgtcgagc aggtagtcga tgcgttcgcg tgccgtaagc tttccctgtt
     3361 cgtgctgctt ttcgatgcgg gattttcctc cgccttcgga aacttttccg aaacgctttt
     3421 ccatcgcgga gatgagcagc ttcacggtgt cctcgttctt gttgaaggta atgtccactg
     3481 ttgatttttc aggaggcata cgtcaggatg taaatgatga gagccaaagt taacatttta
     3541 gcagggaggg aaaagacata aaaaagcccg gaaaaaactt ccgggctcaa ctgtaatatc
     3601 ctgttgtgtt atcgtgtgat cacaagccgt tgtgtgctcg cgcctttcga atctgtggaa
     3661 cggacaaaat agatcccgtc cggaagtccc gcaacgctga tctcctgtgt tccgggtgtg
     3721 atgtcgaggt tcatgacgag atcgccggtg gcgttgaaga tcatcacaga agttctttct
     3781 gctgcggtga tcacgagctt atcgttggcg ggattgggat aaagcgtaac accggaagcg
     3841 gccgtgtgct cttcgattcc cgtgcaggca ttcaccgtga tcgttacagt cgatgtggcc
     3901 gaacatccgt tcacatccgt gtaagaatag gtgacaacct ggttgccgtt accggcagcg
     3961 gaaggatcga agctgctgcc cgtgacgccg tttccggaga aggttccgcc tgcaggcgtt
     4021 cccgtcagtg aaacgttcgc gtcgtcaaag cagacattgg ttgaagttgc gctgcttgtt
     4081 accgtgggaa gcgcattgac cgtaaccatg atcgtgtcgc ttgcagagca gccgctggtg
     4141 tccgtcacgt cgacaaaata attgccgctg gttgttacgt cgagcgtctg gttcgtggag
     4201 gcatcgctcc acatataggt cgagccggca ttcagggcat cgagcgtaag catattgccc
     4261 gcgcagaatg cggtgtcatt gccaaggttc acaaccggta ttgcattgac agaaagcatc
     4321 acggtatcgg aactgctgca tccgttcatg tcggtgacat cgacgtaata agttccagcc
     4381 ataccggtca taagcgtctg cgcggtggaa gagtcgctcc acaggtaaga agatcccgcg
     4441 ttcatcgcgt cgaacgtgat catgctgccg gaacagatcg cggtgtcatt gccgaggttg
     4501 accaccggga gcgggtttac gccaagcata atggtgtctg aattactgca tccgttcacg
     4561 tcggtaacgt cgacaaaata cgttcccgcc gttcctgtca taagcgtctg cgtggtggaa
     4621 ccgtcattcc acaggtaaga ggaaccggcg ttcatcgcgt cgaacgtgat catgttgccg
     4681 gaacatacag ctgtgtcgtt gccgagcgaa atgaccggca gcgggttgat gttcagcacc
     4741 accgtatcag aggaggagca gccgatcgga tcggttacgt tcacataata cgttccggca
     4801 atgcccgtgc tcagtgtctg cgcggtcgaa gcatcgctcc acaggtatgt atcaccggca
     4861 ttcatcgcgt cgaacgtgat catgccgccg gaacagatcg ctgtgtcgtt gccgagcgat
     4921 acgacaggta ccgggttgat cgtaacgtca acgccggtgc ccagggaatc agctccgcat
     4981 tcgtcggaaa cccagacatt gtaatgcccc gcttgcgcag tggtgtagga aatggagttt
     5041 gcgccgggaa ttgccacgcc atctttatac cattggaatg aaacagaagt gctggcgccg
     5101 gaggtaagca ctgttgaatc gccggaacag atgttcagcg ttcctgcgat cgcgcgcgag
     5161 cgggtgatga aaacagttcc gcgtatatcg tcgggagcgc tgtttccgta tgtagcgtaa
     5221 acgttcccgg aggtgttcca gagctggaaa cgcgagctga tattgctgcc gatgaaaacg
     5281 ccattgccgc ggtac
//
LOCUS       DASTSF010000335         3309 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_687885,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000335 DASTSF010000000
VERSION     DASTSF010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3309
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_687885"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..565)
                     /locus_tag="VFU15_09545"
     CDS             complement(<1..565)
                     /locus_tag="VFU15_09545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718067.1"
                     /translation="MSTENKTIGYIFPEADKFLKEKLLGMRKTAIKLFGTLGRQNKPL
                     PDDTDIRSTVATIKAGHEHLFAEEMHHIKPEVHHAAFRSREKETDQKRAALDDEAEKL
                     THNIITAEHKIEGKTRPKTRKRSPWAVAFLILLYFGETVLNSLALEVGGGMPIWAAIL
                     AAAITGIEAVIAYRIARNIERLEAEGKK"
     gene            complement(607..1077)
                     /locus_tag="VFU15_09550"
     CDS             complement(607..1077)
                     /locus_tag="VFU15_09550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718068.1"
                     /translation="METTTITMTDEEAINLYFRDTYKRWQDGEPCMDAMAEIKGEKAD
                     LKIGEVIYHHKDGRTTYSGKDCDGNEIFPETPSLPYAKLQFFRRRLKFVNLAKARQEE
                     RSLDPLQSKNSEIIVTRTEPIPSPYQDGKEAKAEKRTHTIKRTVSRSQKKSRGR"
     gene            complement(1108..1440)
                     /locus_tag="VFU15_09555"
     CDS             complement(1108..1440)
                     /locus_tag="VFU15_09555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718069.1"
                     /translation="MRFSDFDAEDSSIASVALIDGQKVAAYIYREYNETTRRMMYRVY
                     DTHGKEILPMRASLHEQKRKLKDMEYDLSLGIGAHMLNEHTRPKPRRRRIERKLRTLG
                     RGTRSYGR"
     gene            complement(1569..2051)
                     /locus_tag="VFU15_09560"
     CDS             complement(1569..2051)
                     /locus_tag="VFU15_09560"
                     /inference="COORDINATES: protein motif:HMM:NF018552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 domain-containing protein"
                     /protein_id="HEU4718070.1"
                     /translation="MEYDPLNRTIYRKLCTVIPNLPEHLKAGTEYGKSALGKSGLMDL
                     SYDYILEEKPGEHLIALGHRYEQNGDLVPDPDMEIRVIPIKEIAEALSFNNIFLYQHV
                     ESNEKKNEKLQRDLNEFLDQWLDACIAHGHRIELSKVKSREDDLDDLRSEEREPGIER
                     "
     gene            complement(2691..>3309)
                     /locus_tag="VFU15_09565"
     CDS             complement(2691..>3309)
                     /locus_tag="VFU15_09565"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEU4718071.1"
                     /translation="GANNDLGYSLSADRTGCVYVAGEFELDTITFDGYELIPDSSYTD
                     PMFMVKYDPAGNVLCASLLDCGGGGASVSSDLFGNAYASGAFNCHSFTVDTTVLAANS
                     VWDIFLAKYRCENGVAVPDLSSPGEFSAFPNPAGDFITFRLPDLSTSATLIITDNLGR
                     EVKRQQLNGEKQVQISVADLADGIYFYRFTGENGIVSTGKFIIQR"
BASE COUNT          696 a          793 c          898 g          922 t
ORIGIN      
        1 gctttttgcc ttccgcttcc agccgttcga tgttgcgggc gatacggtat gcgatcacgg
       61 cttcgatgcc ggtgatggca gcagcgagaa tagcagccca gatcggcatt cccccgccaa
      121 cctcaagcgc caacgagttc aaaacagttt cgccgaagta aagcagaata aggaacgcca
      181 ccgcccacgg gcttcgcttg cgtgttttcg gccgcgtctt gccttcgatt ttgtgctcgg
      241 ctgtaatgat gttgtgcgtg agtttttccg cttcgtcatc gagcgcggcg cgtttttggt
      301 ctgtttcctt ttcccgcgag cggaacgcgg cgtgatgcac ttcgggcttg atgtggtgca
      361 tttcttctgc aaaaagatgt tcgtgtcccg ctttgatcgt tgcaacagtt gaacggatgt
      421 ccgtgtcgtc gggcaggggc ttgttctgcc tgccgagtgt cccgaacagt ttgatggctg
      481 tctttcgcat cccaagcagt ttttccttga ggaacttgtc agcttcggga aaaatgtagc
      541 cgatggtttt gttttctgta ctcatgtgta ctgtgtgtta tatgggttga atggataaat
      601 gtttgttcac cgtccgcgtg attttttctg agagcgggaa acagtgcgct tgatcgtgtg
      661 cgtgcgcttt tcggcttttg cctctttgcc gtcctggtac gggcttggga tcggctcggt
      721 gcgcgtgacg atgatttccg aatttttcga ttgcagcgga tcgagtgacc gctcctcctg
      781 ccgcgctttc gccaggttca cgaacttgag gcggcggcgg aagaattgca gcttcgcgta
      841 tggcagcgat ggcgtttcgg gaaaaatctc gttaccatcg caatcttttc cgctgtaagt
      901 cgtccggcca tctttgtggt ggtagatgac ttcgccgatc ttgaggtctg ccttttcgcc
      961 ttttatttcg gccatggcgt ccatgcacgg ctcaccgtct tgccagcgct tgtaggtgtc
     1021 gcggaaataa aggttgatag cttcctcgtc ggtcattgta atcgttgtcg tttccatgtg
     1081 ttgcgagttt tggtttgcat tgttgagtta tctgccgtag ctgcgcgtac ctctgccaag
     1141 cgttcgaagc tttcgctcga ttcgtctgcg gcgcggtttc ggtcgcgtat gttcattgag
     1201 catgtgtgcg ccgatgccaa gcgagaggtc gtattccatg tctttcaatt tgcgcttctg
     1261 ttcgtgcagg cttgcgcgca tcggcaagat ttccttgccg tgcgtgtcgt acacgcggta
     1321 catcatgcgc ctggtcgttt cgttgtattc gcggtagata tacgcagcga ctttctgccc
     1381 atcgatgagc gccaccgatg cgatgctgct gtcttcggca tcgaagtcgg aaaagcgcat
     1441 gcggctttct tgtaatggtt gtgggagctg tgtgctcatg gtgatttgtg cgtgaactga
     1501 taatgcctgt gcttttgctt acatacccgc aatccctctt gaaatgacac cgcggtgcaa
     1561 aaaatttctt accgctctat tcctggttcg cgttcctcgc tccgcaaatc gtcaaggtca
     1621 tcttcgcgtg atttcacttt cgacaactcg atgcggtgtc cgtgtgcgat acaggcgtca
     1681 agccattggt caaggaattc gttgaggtcg cgttgcagct tctcgttttt cttttcgttg
     1741 ctctcgacat gctgatagag aaaaatattg ttgaatgaaa gggcctctgc gatttccttt
     1801 atgggaatca cacggatttc catgtcggga tcgggaacca agtcgccgtt ttgctcgtaa
     1861 cggtggccaa gtgcgatcaa atgttcgccg ggcttttcct ccaggatata gtcgtatgac
     1921 aagtccatga gaccggactt accaagcgca gacttgccgt attccgtgcc tgccttcaag
     1981 tgttccggca ggttcggaat gaccgtgcag agtttgcggt atattgtccg attgagtggg
     2041 tcgtattcca ttgaatgtgg tgcactaagt agtaatgccg cgtgtaccga tacataccgc
     2101 ggaagagcgc agtgatgaca gcgtgcgtcg taaaagtttt aacttggaat tcgtttggga
     2161 aatcctttcg aagactgcgg aatcagcgcg gaatttcagc gcggaagtac cctgaaaagc
     2221 atcttttgtt cgccgctgtc acaaacgtaa aacgctaacc gacaggcttt caatttcatc
     2281 ccctatccct gttaatcatg gggtccctgg ttcgagccca ggagggggag cgatgaaaac
     2341 catcttgtaa aaacgggatg gtttttttgt ttttgagaag cgacggttag agcatccgcc
     2401 gcggcggatt aatcatgggg tccctggttc gagcccagga gggggagctt cttaagacaa
     2461 acacccgata gatgaatttc tgccgggtgt ttttgttttg ttccgcttcg atggaggtag
     2521 caatggaggt cacaggttcg gaaatagggg acatcttctg aatgcaactt tcacgagcaa
     2581 cgttcttccg ccggtagtcc gggaagcaac gaacggcacc catatagaat gccgtttagg
     2641 tttgatagaa gaagtatagc gtgtcaggga ataaagccag gtagtggcca ttaacgctga
     2701 atgatgaatt tcccggttga aacaatacca ttttctccgg tgaagcgata aaaatatatt
     2761 ccatccgcca aatccgcgac ggaaatctgc acctgctttt ctccgttcaa ttgttgtcgc
     2821 ttcacttccc tgccgaggtt gtcggtaatg atgagggtgg cggaagttga aaggtctgga
     2881 agtcggaaag tgataaaatc gcccgctgga ttagggaaag ccgaaaactc accgggagaa
     2941 gaaagatcag gaacagctac accgttttca cagcgatatt ttgcgagaaa tatatcccat
     3001 acagagtttg ctgccaagac agtcgtgtca acagtaaaag aatgacagtt aaaagctcca
     3061 ctggcgtatg cattaccaaa aagatccgaa gaaacgctgg ctcccccacc tccgcaatcc
     3121 agcagggagg cgcataatac attgccggcc ggatcatact tcaccatgaa catggggtct
     3181 gtataagagg agtccgggat caattcataa ccatcgaatg taattgtgtc cagttcaaat
     3241 tcaccagcga cgtatacaca gcccgttcgg tctgcactga gcgaatagcc gagatcatta
     3301 ttggcccca
//
LOCUS       DASTSF010000336         2739 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_495527,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000336 DASTSF010000000
VERSION     DASTSF010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2739
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_495527"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2715)
                     /locus_tag="VFU15_09570"
     CDS             complement(<1..2715)
                     /locus_tag="VFU15_09570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718072.1"
                     /translation="MIIKQSSKEAGETSLGLSIGTAFNSRAGVRQLSINTELSNSYRK
                     DLNKKRGNDELYAGSSLGLFGTSSSFSFGTYTYSPKVDMPMKNFSVTGNFKFGGEFFG
                     IFGNYTLGGYFSTQRLETTSVSSPAYGYMNYDAGTYNSSALMDFNRENDGAFSQNTPA
                     LPLTSLTYDIYSVSGQGVGGSYRPFRSDVGFVYDNYNYTTSDGLSIGAELGTANLVHW
                     GGDISVNSTNTYSGWWTVNNSAASYLHFKPASSASDYEPFYFKEANEKSVDSDPTFVS
                     GFGGTNAERFNLDHVSKFNTRADAQLVDKYGNVTSLPSSNTRRKRDRRNQVIYQLTRD
                     EVDAGYGLCDPEAGAYSAPGHHIAEITTYGTDGMRYVYGLPAYNTEQDEVTFATGEKL
                     DGSGGHTGNCTTGLVGYSANTDNSVDNTLGIDNYYSRTSTPAYAHSYLLTAVLSPDYI
                     DADNVKGPSINDLGYYTRFYYSKVDDYHWRVPVQADTATWNQGLYSDPNDDKGSYIYG
                     KKELWYLDSIVSKNYIAIFTKENRKDGFGVVDENGGIQTSSSKAMKLLRKISLYSLTD
                     YHNNGSGAVPIKEVHFEYDYSLCPGVPNNSGASETVNSVNLNANKGKLTLKKVYFTYR
                     NSNKARLSPYQFSYNSINPSYDLKGYDRWGNYKPNVTSCGSTADLNNAEYPYVSQDQA
                     QADSCSSAWSLTDINLPSGGKISITYESDDYAYVQNKNAMEMFRIVGVEDDYGHMDMS
                     SSPADKILSNSVRKNRRIYFKMQSGYNDINSYFQGISRLYFRCLMSFDPNTDRHDFVP
                     GYCNIDSYGDTTVSGTHYGWIRLQPVKLKDSGSSDYNPIVKSAIQFGRMNLSRFIWDQ
                     TAVPVENGLSEDVLNLMVNSSFVKNIFDAIEGPNKAIWDKDRGRSITVN"
BASE COUNT          611 a          686 c          697 g          745 t
ORIGIN      
        1 attgaccgtg atcgatctgc cgcggtcttt atcccatatc gctttgttcg gaccttctat
       61 cgcgtcgaaa atgtttttca caaaagagga gttcaccatc aggttcaaca catcttcgct
      121 gagcccgttc tcaaccggca ccgctgtctg gtcccagatg aaacgggaaa gattcatccg
      181 tccgaactgg atggcggact ttacgatggg gttgtagtcg ctgctgccgc tgtccttcag
      241 cttgacaggc tgcaacctga tccatccgta atgtgtcccg gaaactgtgg tgtcgccata
      301 cgaatcgata ttgcaataac cgggaacgaa atcgtgcctg tccgtgttcg gatcgaagct
      361 catgagacag cggaagtaca gcctggaaat tccctggaaa taagaattga tgtcgttgta
      421 tcccgattgc attttgaaat aaatgcgacg gtttttgcgc acgctgttgc ttaatatttt
      481 atccgccggt gacgaagaca tatccatgtg cccgtaatca tcttccacac cgactatcct
      541 gaacatctcc atcgcatttt tattctgcac atacgcgtaa tcatccgact cgtacgtaat
      601 gctgatcttt cccccggacg gaaggtttat atccgtcagg ctccatgccg atgaacacga
      661 gtcggcctgc gcctggtcct ggctgacgta gggatattcc gcattgttca ggtcggcggt
      721 gctcccgcag gaagtcacgt tcggcttgta attgccccac cggtcataac ctttgaggtc
      781 atacgacggg ttgatcgagt tgtagctgaa ctggtatgga cttagccgcg ccttattcga
      841 attgcggtaa gtgaaataaa ctttcttcag tgtaagtttt cctttattcg cgttaagatt
      901 aacgctgttc accgtttcgc tggcgccgct gttgttggga acgccggggc aaagcgaata
      961 atcatattcg aaatgcactt cttttatcgg aactgcgccc gatccgttgt tatgataatc
     1021 ggtcagtgaa tagaggctga tcttgcgcag cagcttcatc gctttcgaag agctcgtctg
     1081 aattcctcca ttctcatcaa cgacaccgaa accgtccttg cggttttctt tcgtgaagat
     1141 ggcgatgtag tttttcgaaa cgatggaatc gaggtaccac aattctttct tgccgtagat
     1201 gtagctgccc ttgtcatcgt tagggtcgga atacaagccc tggttccagg tagcggtatc
     1261 tgcctgcaca ggaacgcgcc agtggtaatc gtccactttc gaataataga atcgcgtgta
     1321 atacccgaga tcatttatgc tgggaccttt cacattgtcc gcatcgatgt aatccgggct
     1381 gagcaccgcg gtcagtaagt acgaatgggc gtaagcaggc gttgaagtcc ggctgtaata
     1441 attatcgata ccaagcgtgt tatcaaccga attatccgtg tttgccgaat agcccacgag
     1501 accggttgtg caatttcctg tgtggccgcc gctgccgtcc agcttttctc cggtggcgaa
     1561 tgtgacttca tcctgttcgg tattatacgc gggcagacca tatacataac gcattccgtc
     1621 tgtgccgtat gttgttattt ccgctatgtg atgtcccggt gcgctgtatg cgcctgcttc
     1681 cggatcgcaa agaccatacc cggcgtccac ttcatcacgg gtcagctggt agatgacctg
     1741 gttgcgccgg tctcttttcc tgcgtgtgtt cgacgatggg agggaagtaa cattgccgta
     1801 tttatccacc agctgcgcat cggcgcgtgt gttgaatttt gaaacgtggt cgaggttgaa
     1861 tctttctgca ttcgttccac cgaatccgct tacaaaagtg ggatccgaat cgaccgattt
     1921 ttcattcgct tccttgaaat agaatggctc ataatccgac gccgaagaag ccggcttgaa
     1981 atggagatag gatgcggcgg aattatttac cgtccaccag cccgaatacg tattcgtgct
     2041 gttcacgctg atgtcgcctc cccagtgaac gaggttcgcc gttcccagtt ctgcaccgat
     2101 ggaaagaccg tcactggtgg tgtaattgta attgtcatag acaaaaccga catcggaacg
     2161 gaacgggcgg tagcttcccc ccactccctg gccggacaca gaatagatgt catacgtcag
     2221 gctggtaagc ggaagcgcgg gtgtattctg cgaaaaggcg ccgtcgtttt cccggttgaa
     2281 atccatgagc gcggagctgt tatacgtacc cgcatcgtaa ttcatatacc cgtaagccgg
     2341 tgaggaaacg gaagttgttt cgagtcgctg tgtgctgaaa tagccgccca gcgtatagtt
     2401 gccgaaaatg ccgaagaact ctccgccgaa tttgaaattc ccggttacgg aaaagttttt
     2461 catcggcatg tcaactttgg gcgaataagt ataggtgccg aatgaaaacg aagaggatgt
     2521 gccaaagagg ccgagcgaac ttccggcata gagctcatcg tttccccttt ttttattaag
     2581 atctttccgg tagctgttgc tcaactccgt gttgatcgaa agctggcgga cacctgcgcg
     2641 ggagttgaat gctgttccga tcgataatcc gagacttgtt tcgcccgcct ctttgctcga
     2701 ctgtttaatt atcatgctgt agctaaggcc gggttgaat
//
LOCUS       DASTSF010000337         6399 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_2564,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000337 DASTSF010000000
VERSION     DASTSF010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 6399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6399
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2564"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..511
                     /locus_tag="VFU15_09575"
     CDS             <1..511
                     /locus_tag="VFU15_09575"
                     /inference="COORDINATES: protein motif:HMM:NF027798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-1,3-glucanase family protein"
                     /protein_id="HEU4718073.1"
                     /translation="TAPTQTAPTLSAGKSTLTPGTKYYYIVTATNANGETYASLEFEA
                     TPTAENGCVNVNWQPMSAATTAINIYRGTESFGENVLVQQVANTGGVSSMIDSGQSQN
                     APQSPPMYFPSGVPSSAYDQFFHLTNISVNGAAYAGPYDDQGQQSSTISTGNPASATI
                     TLGWERPAR"
     gene            complement(610..2589)
                     /locus_tag="VFU15_09580"
     CDS             complement(610..2589)
                     /locus_tag="VFU15_09580"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEU4718074.1"
                     /translation="MKNRVLLSFALCLAGISANAQTIFTENFESAPNSMPAGWHQQLP
                     QFDPTNQGWQFGTTIGGTLAPYVPPHTHYAFVNDLDNNSSYSFNRDTLYTPVLDFSSY
                     SSVFISFDYWYYGGFYFNPGDYEIASIAASGDGGHTWTTIDTMGCWETSAWRTGLYDL
                     SAFAGNANVMLAFTYDDLANDANGFVVDNINVYAPIGYDAGGRSTDMFYLCKTNNTYP
                     VTGLLHNFGGNTISSMHLKYTVNNGPVQSDYMSALSIAPLTDYYFTHSIPWTPSVAGD
                     YILRIWADTLNGPHDDQLHTNDTLYAYFVVVDTLQAKEPLLEEFSQASCDPCMHATPN
                     LDSVLFNNQSTCNALRYHVSYPGLDYMNNVTQASSVGPRVSYYGIQGVPDARIDGVTD
                     VYPGLVSSADLQSARAQGSPFTITVSAAWDPANHQYTATAYITAHGDLPAGLTARAVL
                     TVDTMKYHNDQSQEDPPSVFAPPIGTGSIPDFYYPYTLNFPEVVEDIMPNAAGTSLGA
                     FTSGQTQVVNFSWTKDHPWASNGNTWLYDSTDVHITVFIQNDGDLQAGWGALPGYIYQ
                     SASDAIITTGIAENDPQEKISVYPNPSGGIFTLHNSFAGTYETAVFNSLGAEVYHSVT
                     DENDLHIDLSGQPAGIYFVRTVANGNVYSVKIVKQ"
     gene            complement(3067..3849)
                     /locus_tag="VFU15_09585"
     CDS             complement(3067..3849)
                     /locus_tag="VFU15_09585"
                     /inference="COORDINATES: protein motif:HMM:NF013357.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH25 family lysozyme"
                     /protein_id="HEU4718075.1"
                     /translation="MTLRSNGINRLAECTAFAAVLLFTACTGGQTENKTDTAAKKPDT
                     THVVQEVKNTPPKNYYGIDISQWNGNIAEDLPKVDSLTFMICKATEGIDFVDPDFYNN
                     WKMVKEKGFIRGAYHFYHTDDDPLKQAEFFLSKVKDIDSTDMPLIIDIEQESLPQGAQ
                     VDMQKLQSDLLTFMQQLEAKSKRIPILYTDYAFANMYLVNDVFAKYPLWLAEYSGGST
                     PLIPQTWKNAGYMIWQKTDHYDVNMNAVDFDEFHGEKTSLTD"
     gene            complement(3985..4461)
                     /locus_tag="VFU15_09590"
     CDS             complement(3985..4461)
                     /locus_tag="VFU15_09590"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEU4718076.1"
                     /translation="MKTVKEIMVKSPMYCEKDATLQAVAEQMSKENIGSLPVLDENKK
                     LIGMITDRDICLAAGATKKNLASLKVQEVLKQQPVHTITGEDDLRSALRLMRVKQVGR
                     LPVVDKDRRLQGVVSLNHIVRRTHGTADAAEIEHGGDENVLNTLHALSNRKLEMAL"
     gene            4775..5188
                     /locus_tag="VFU15_09595"
     CDS             4775..5188
                     /locus_tag="VFU15_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015324936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEU4718077.1"
                     /translation="MKPFKTTDAYLAALPEEPRATLEKIRAAVRAAAPDAEEVISYGM
                     PAFRLNGRLLVSFSAFKNHCSFFPMSKSVIRILGDALKKYPTNPGTIRFPIGKPLPAT
                     LVKKMVKMRIRENEERELLRALKKTTKKKTASGKK"
     gene            5207..5632
                     /locus_tag="VFU15_09600"
     CDS             5207..5632
                     /locus_tag="VFU15_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011556185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU4718078.1"
                     /translation="MQKIVPFLWFDNNAEDAIRFYTSVFKDSKIIHLSPMVSIFELNG
                     QQLMAINGGPQYKLTEAFSLMVNCETQEEVDYYWEKLCEGGSASRCGWLKDKYGLSWQ
                     IVPTVLGKLMSGNDPERAKRVMNAVLQMSKLDIATLESA"
     gene            5734..>6399
                     /locus_tag="VFU15_09605"
     CDS             5734..>6399
                     /locus_tag="VFU15_09605"
                     /inference="COORDINATES: protein motif:HMM:NF033679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNRLRE domain-containing protein"
                     /protein_id="HEU4718079.1"
                     /translation="MRSFLLSAALLAAFSVTAQQNITVYQPGPGMNDSTDQGGLNGGK
                     DTWDYDGMPGSNYAQDIYFYATPISNCNQTHVRCYIQFDLSTLPFNPDSVFFGVTHYD
                     HTSYCYSNCDADFYFHAITSPWYEQTLTYYNTPSIDTAFYGPINITFPNSFGQREYNI
                     TAMYNLWKDGVVPNYGFEIESNTVGCNNAAVMFYAHSSDDTSAAARPYLKVYSSATGV
                     VEKN"
BASE COUNT         1520 a         1683 c         1660 g         1536 t
ORIGIN      
        1 aaccgcgccc acacaaacgg cgcccacatt atccgcaggt aaaagcacgc ttacgcccgg
       61 aacgaaatat tactacatag tgacggcgac caatgccaac ggggaaacat acgccagtct
      121 cgaattcgaa gcgacgccca ccgcggagaa cgggtgcgtc aatgtcaact ggcaaccgat
      181 gagcgcagcc acgaccgcta tcaacattta ccgcggcacg gaatcgttcg gcgaaaatgt
      241 ccttgtgcag caagtggcca acacgggagg cgtatcaagc atgatcgact ccggccagtc
      301 gcagaatgct ccgcaatctc cgccgatgta ttttccttcg ggcgtcccgt caagcgccta
      361 tgatcagttt tttcacctca cgaacatcag cgtgaacggc gcagcttatg caggtccgta
      421 tgacgaccag ggacagcaat cttccaccat ctccaccggc aatcctgctt ccgccacgat
      481 cacgctcggg tgggaacggc cggcacgata aattccgcga ttgtcccggt atgaatagct
      541 ctccgtcagc ggagagcttt ttattttttt catggaaatt ttttcctgca agtgcggcgc
      601 cgtattcttt tactgcttca cgatcttcac ggaatacacg ttgccattcg ccacagtgcg
      661 cacgaaataa attcccgccg gctggccgct gagatcgatg tgcaggtcgt tttcatccgt
      721 cacgctgtga tacacttctg cgccaagcga attgaagaca gctgtttcgt acgttccggc
      781 aaatgaatta tgcagcgtaa aaattccgcc cgatggattc gggtagacgg aaattttttc
      841 ctgaggatca ttttccgcga taccggtggt gatgatcgca tcggaagccg attggtaaat
      901 gtaacccggc agcgctcccc acccggcctg caggtcgccg tcgttctgaa tgaaaacagt
      961 aatgtgcacg tcggtggaat cgtaaagcca ggtattcccg ttgctcgccc agggatgatc
     1021 tttcgtccag ctgaaattca cgacctgcgt ttgcccgctg gtgaatgcac cgagacttgt
     1081 tcctgctgca ttgggcatga tgtcctccac cacttcggga aaattcagcg tatacggata
     1141 gtaaaaatcc gggatcgacc cggtcccgat cggtggtgca aaaacagaag gcggatcttc
     1201 ctggctctgg tcgttgtgat atttcatcgt gtcgaccgtg agcaccgcgc gtgcggtaag
     1261 tcctgcggga agatcgccgt gcgcagtgat gtaggcagtg gccgtgtact ggtgattcgc
     1321 aggatcccac gcagcgctga cggtgatcgt gaacggggaa ccctgcgcgc gcgccgactg
     1381 caggtcggca gaagaaacaa gcccgggata aacatccgta acgccgtcga tccgcgcatc
     1441 cggcacaccc tgtattccat aatacgaaac acgcggacca acagaagagg cctgcgtgac
     1501 gttattcatg taatcgaggc cgggataact gacatggtag cggagcgcgt tgcaggtcga
     1561 ctgattgttg aacagcaccg aatcgaggtt cggtgttgcg tgcatgcacg gatcgcacga
     1621 cgcctgtgaa aattcttcca gcagcggttc tttcgcctgc agcgtatcca cgactacgaa
     1681 atacgcatac aaggtgtcat tggtgtgcaa ctgatcatca tgcggaccgt tgagcgtgtc
     1741 ggcccagatg cgcagaatat aatcaccggc aacggaaggc gtccacggaa tgctgtgggt
     1801 gaaataataa tctgtcagcg gtgcaatgga aagcgcgctc atgtaatcac tttgcacggg
     1861 accgttgttc accgtgtatt tgagatgcat cgagctgatc gtattgcctc cgaaattgtg
     1921 cagcagcccg gtaaccgggt aggtgttatt cgttttgcag agataaaaca tatccgtgct
     1981 ccgtcccccg gcatcgtaac cgatcggtgc gtaaacattg atgttgtcca cgacgaaacc
     2041 atttgcatca ttggcaagat catcatacgt gaaagcaagc atcacattcg cgttaccggc
     2101 gaaggcagag agatcgtaaa gacccgttcg ccatgcgctt gtttcccagc aacccatcgt
     2161 atcaatcgtt gtccatgtat gaccgccatc gcccgaagcg gcaatagacg cgatctcgta
     2221 atcgccggga ttgaaataga aaccgccgta ataccaatag tcgaatgaaa tgaaaacgct
     2281 tgaataactg ctgaaatcaa gtacgggcgt atagagcgtg tcgcggttga atgagtagga
     2341 agaattatta tcgagatcat tcacgaacgc gtagtgcgta tgcggaggca catagggcgc
     2401 gagcgttccg ccgatcgtgg tgccgaactg ccatccctgg ttggtcggat cgaattgcgg
     2461 cagctgctgg tgccagcccg cgggcatgga attgggcgcc gattcgaaat tctctgtaaa
     2521 gatcgtctgc gcgttcgcgg aaatgccggc aaggcacaaa gcgaaagaaa gaagtacccg
     2581 gtttttcatt attgcaggaa tagaaggtaa gacaggcggg agaatgttgc tctatcaagt
     2641 atacaacttg cgccccggaa aagcaaaagc gggatcgctt aacatgcacg cggtgcattt
     2701 aagaatgtca tcttttgaaa gatcgattct gtttctaaat gaccggaaaa attccccgac
     2761 acacatccac gacgcccacc cggttgctgt gttttggaaa tctcacttta tataacgaat
     2821 gaaaaacagg acagcgccat agctggtttt ccgcagaatt cgagttgcag aaaagcagcg
     2881 gaggaatgtg tgtaagcaac cccggaagtg aagcgaagcg gaacggcttg ggttgcgggc
     2941 gcttttcgaa acgtagaatt caggaaaatc agctccgggc gcgctttttt gcgcaacttt
     3001 tttctaaaaa aagttgcagg gaaaaacaaa aacgacagcc tttcaataat gaaaaattac
     3061 gcccgctcag tcggtaagcg aagtcttttc cccgtggaac tcatcgaaat cgacggcgtt
     3121 catgttcacg tcgtaatgat cggttttctg ccagatcatg taacctgcat ttttccatgt
     3181 ctggggaatg agcggcgtgc ttccgccgga atattccgcc agccacagcg ggtatttcgc
     3241 gaacacgtcg ttgacgaggt acatgttggc gaatgcataa tcggtgtaga gtatgggaat
     3301 gcgtttactt ttcgcttcga gctgctgcat gaacgtgagg agatccgact gcagcttctg
     3361 catatccact tgggcgccct gcggaagact ttcctgttcg atgtcgatga tgagtggcat
     3421 gtcggtcgag tcgatgtctt tcactttgga aagaaagaac tccgcctgct tcagcggatc
     3481 atcgtccgtg tggtaaaaat gataggcgcc gcggatgaac cctttttcct tcaccatttt
     3541 ccagttgttg taaaaatcgg gatcgacgaa gtcaatccct tccgtggctt tgcagatcat
     3601 gaacgtgagg ctgtccactt tcggaagatc ttccgcgatg ttcccgttcc actgggaaat
     3661 atcgatgcca taataatttt tcggaggggt gtttttcact tcctgcacca cgtgcgtggt
     3721 gtccggtttt tttgccgccg tgtcggtttt gttttccgtc tgaccgccgg tgcaggcggt
     3781 gaaaagaaga acggctgcga aagcggtgca ttccgccagc cggtttattc cgtttgatct
     3841 gagtgtcata aagcagggat tgaatttccg gtgtgaaaaa taggaaatcc ttttcgaacc
     3901 ccgcaacgca gaaaagcgcc tgctgctgca cagacgcttt tccgttcaac gttccatccc
     3961 tgctgcggca aggtgttttc ctgctcacaa cgccatttcg agcttccggt tcgaaagcgc
     4021 gtgcagcgtg ttgagcacat tttcatctcc gccgtgctcg atctccgcag catccgcggt
     4081 tccgtgcgtg cggcgcacga tgtggttcag ggaaacaaca ccctgcaggc gccggtcttt
     4141 gtcgacgaca ggcaggcgtc cgacctgctt tacgcgcatc aggcgcagcg cagaacgcag
     4201 gtcatcctct cccgtgatcg tgtgaacagg ctgctgcttc agcacttcct gcactttcag
     4261 cgacgcaagg tttttcttcg tcgcacctgc ggcaagacag atgtcgcggt cggtgatcat
     4321 gccgataagt tttttattct catccagcac gggcagtgag ccgatgttct ctttcgacat
     4381 ctgctccgct actgcctgga gcgttgcatc cttttcgcag tacatgggtg attttaccat
     4441 gatctctttt accgttttca ttttcgtgtg tatttgtaaa tgatcccggt tatgtgcgga
     4501 accgggatta ccgcgaggtc atttgtgttc ttccgtttct tttccgttgc agcggagtac
     4561 aaatctactt ttcatgctgc cgctccgttc gttttttcga tcccctgcgg taatgaaacg
     4621 atgcatggaa atgccccgct aaaacatgta gcggcatcgt tcgccggaaa atcgcttcac
     4681 gaaaggaaca cggcggatca tgtaatttta tcttaacggt tatcctgttt tcctgttaat
     4741 cctaacttgg gcgtgttttt cagcttgccg gatcatgaaa cccttcaaaa ccaccgatgc
     4801 ctatctcgcc gcgctgcccg aggagccgcg cgccacgctc gaaaagatcc gcgctgccgt
     4861 gcgcgccgcc gcgcccgatg cggaagaagt gatcagctac ggcatgcctg cgttccgcct
     4921 caatggaaga ttgctcgtca gcttttctgc atttaagaat cactgctcgt tcttcccgat
     4981 gagcaaaagc gtgatccgta ttctcggcga tgcgctgaaa aaatacccga ccaacccggg
     5041 cacgatccgt tttcccattg ggaaaccgct tccggccacg ctcgtgaaaa agatggtgaa
     5101 gatgcgcatc agggaaaatg aagaacgtga gctgctccgc gcgctgaaaa aaaccacgaa
     5161 gaaaaaaaca gcatccggaa agaaataacc attcataaaa acagtcatgc aaaaaatcgt
     5221 tccctttctc tggttcgaca acaatgcgga agatgccatc cgtttctata cgtccgtttt
     5281 caaagattcg aaaatcatcc acctctcccc gatggtatcc atcttcgagc tgaacgggca
     5341 gcagctcatg gcgatcaacg gcggaccgca atacaagctc accgaagcgt tttcgctgat
     5401 ggtgaattgc gaaacgcagg aagaagtgga ttactactgg gaaaaacttt gtgaaggcgg
     5461 atcagcgtcg cgctgcggat ggctgaaaga caaatacggt ttgtcctggc agatcgttcc
     5521 gaccgtgctc ggtaaactga tgtcgggcaa cgacccggaa cgtgcgaagc gcgtgatgaa
     5581 tgccgtcctg cagatgagca agctcgacat cgccacgctg gaaagcgcat agctgcaacc
     5641 tggccatttc ccgttaacgc agatcaaccc ttcccttgat cattgtcatg gggctgcggt
     5701 cgcatgtgaa agtagttttg aaaaaaattt cacatgagat catttttact ttccgctgcg
     5761 ttgctcgcag cgttttctgt aacagcgcag cagaatatca ccgtttacca gcccggtccc
     5821 gggatgaacg attccaccga ccagggcggt ctcaacgggg gaaaagacac gtgggattac
     5881 gacgggatgc ccgggagcaa ttatgcgcag gatatttatt tctatgcgac gcctatcagc
     5941 aactgcaacc agacgcatgt gcgctgctac atccagttcg acctgtccac gcttccgttc
     6001 aatcccgatt cggttttttt cggtgtgacg cattatgatc atacttccta ttgttattcc
     6061 aattgcgacg cggactttta tttccatgcg atcacatcgc catggtatga gcagacgctg
     6121 acgtattaca atacgccgtc catcgatact gcattttacg gtcccatcaa catcactttt
     6181 ccgaacagct tcgggcagcg cgaatacaac atcacggcca tgtataacct gtggaaagac
     6241 ggcgttgttc cgaactacgg cttcgagatc gaaagcaata ccgtcggctg caacaatgcc
     6301 gctgtcatgt tctacgcgca ttcctccgac gacacatcgg cggcggcacg tccttacctg
     6361 aaggtttatt cttccgcgac cggcgtcgtg gaaaaaaat
//
LOCUS       DASTSF010000338         3246 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_2270,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000338 DASTSF010000000
VERSION     DASTSF010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3246
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2270"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(223..885)
                     /locus_tag="VFU15_09610"
     CDS             complement(223..885)
                     /locus_tag="VFU15_09610"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU4718080.1"
                     /translation="MRITELTLSAPDPSDLRKFYTRLGFKEIEAPGGTDGFSFNIGYT
                     RLNFAPGDRDARYHFAFNVQPDRIEDALAWLEQNGVEVLENRGGKGKLVDFPNWKARS
                     VYFYDTAGNIVEIIARAALPRAGNAPKFSSASLLGVSEIGIVCDNVSSMKQWISSTHG
                     IGDFARQENTEDFTAMGDDEGLLLLVPAGRRWFMGNFDSARFPIALTGQNGGKEIRLI
                     LP"
     gene            complement(1101..2513)
                     /locus_tag="VFU15_09615"
     CDS             complement(1101..2513)
                     /locus_tag="VFU15_09615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014203317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEU4718081.1"
                     /translation="MEYGKITADIISELRRIAGNDFVHTDEERLERYGQDETEDLLFK
                     PSVVVRPGTAEEISALLKLCNAEKIPVTPRGAGTGLSGGALPVHGGVCLSMERFNRIL
                     EIDERNLQATVQPGVINEAFQEAVKEKGLFYPPDPASKGSCFLGGNLAHSSGGPKAVK
                     YGTTRDYVLNMEVVLPTGEIIMTGANVLKYSTGYNLTQLMIGSEGTLGVITKIVFRLL
                     PLPKHNLVMLVPFRSAEKACEAVSAVFREGITPSGMEFMERDAIDWVRKFVDDINIGV
                     PDDVQAHLLVEVDGNDIDVLFKDAEKISEVMQRFGCGEILFADTADQKNMLWKMRRKV
                     AEAVKSNSIYKEEDTVVPRAELPKLLKGVKDIGAKYGFRSVCYGHAGDGNLHVNIIKG
                     DLSDDFWKNELPKGITEIFRLCVDLGGTISGEHGIGLVQQPYIRIAIDEKRIGLMRGI
                     KKLFDPNMILNPGKIFYGKE"
     gene            complement(2539..3129)
                     /locus_tag="VFU15_09620"
     CDS             complement(2539..3129)
                     /locus_tag="VFU15_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HEU4718082.1"
                     /translation="MPGPSKIVIEEYSPQWAEIFLQLKTVFEIKAGKFLSGIHHVGST
                     SVPGLPAKPIIDIDLVIDEKEHFGPVKSILDDLGYDHAGDLGIDEREVFKRRDEFVPW
                     GETQREWPLHNLYVCRNSITALKNHLAFRDYLRENPVKRDEYGALKKQIAAAHPHDID
                     AYVREKTDFVIKALRAKGFSEEELSGIIAQNKAIGR"
BASE COUNT          687 a          902 c          831 g          826 t
ORIGIN      
        1 ttttttttct tcttctcctt tttctttttc ttcttctttt tatgctttcc ggaagatgag
       61 gacgtttccg taccggaatg cattgaatag cgtgtacccg cgttcatcca ctggagcgag
      121 gaaagaagca tgaccgtaac aagaaaaggg aaaatgaacc gggacctcat gatttgcaaa
      181 attccgcatt cctgtaacgc cgttccgtgc gattggttac agctacggca ggattaatct
      241 tatttctttt ccaccatttt gcccggtcag cgctattgga aaacgcgcgg aatcgaaatt
      301 tcccatgaac cagcgtcttc cggcgggaac cagcagcagc aatccttcat catcgcccat
      361 cgcggtaaaa tcttccgtat tctcctgccg ggcaaaatcg ccgatgccat gcgtggaaga
      421 tatccattgc ttcatggatg acacgttgtc gcacacgatc ccgatctcgc ttacacccag
      481 caatgaagct gaagagaatt tcggggcgtt gccggcgcgc ggaagcgcag cgcgtgcaat
      541 gatctcgacg atgttcccgg ccgtgtcata gaaataaacc gaacgcgctt tccagttcgg
      601 gaagtcgacc agctttcctt ttccgccgcg gttttccagc acttccaccc cgttctgctc
      661 cagccaggcc agtgcatcct cgatgcggtc gggctgaacg ttgaatgcaa aatggtagcg
      721 cgcgtcacga tcgccgggag cgaagttcag ccgcgtgtaa ccgatgttga acgaaaaccc
      781 gtccgttccg cccggcgctt cgatctcttt aaaaccgaga cgcgtgtaga atttccggag
      841 atcggaagga tcgggagcgg aaagagtgag ttcggtgatg cgcatacggg tttgcctgtg
      901 cgaatgtaca aattagcttt acagtcctta taagcacaga gttaaagatg aggatggctc
      961 ggcggcaacg aggatatccg ttatttcacg ggatggatcg aatggatggt gaagaccggt
     1021 ggccgcatac gggaagccgg gcagagaaaa agaaaaacgg atgatctccc ggacctgcgt
     1081 ttcacctccc tgtgaccggc ttattctttc ccgtaaaata ttttccccgg gttcaagatc
     1141 atgttcggat cgaagagctt tttgatgccg cgcatcagcc cgattctttt ttcatcgatg
     1201 gcaatgcgga tgtacggctg ctgtacaagc ccgatgccgt gctcgcctga aatcgttccg
     1261 ccaagatcga cgcacaggcg gaatatttcc gttatccctt ttggcaattc attcttccag
     1321 aaatcatcgg aaagatcgcc tttgatgatg ttcacgtgca ggttgccgtc gccggcatga
     1381 ccgtagcata cggaacggaa accatatttc gcgccgatgt ctttcacgcc tttcagcagc
     1441 ttcggcaatt ccgcacgggg aacgaccgta tcttcttcct tgtaaatgct gttcgatttc
     1501 accgcttccg ccactttccg ccgcattttc cagagcatgt ttttctggtc tgccgtatcg
     1561 gcgaaaagga tttccccgca tccgaaacgc tgcatcactt ccgaaatttt ttccgcatcc
     1621 ttaaaaagca catcaatatc gttgccgtcc acttccacga gcaggtgcgc ctgcacatcg
     1681 tccggcacgc cgatgttgat gtcgtcaacg aatttccgca cccagtcgat cgcgtcacgc
     1741 tccatgaatt ccattccgga aggcgttatt ccttcacgga aaaccgcgct caccgcttcg
     1801 cacgcttttt ccgcggagcg gaacgggacc agcatcacga ggttgtgctt cggtaacggg
     1861 aggaggcgga aaacgatctt cgtgatcacg cccagtgttc cctcgctgcc gatcatcagc
     1921 tgagtgaggt tgtaaccggt ggaatacttc agcacgttcg caccggtcat gatgatctcg
     1981 cccgtgggca gcacgacttc catgttcagc acgtaatcgc gcgtggtgcc gtacttcacc
     2041 gctttgggtc cgccggagct gtgcgcgaga ttgccgccga ggaagcagct tcctttcgag
     2101 gccggatcgg gcgggtagaa caatcctttt tctttgacgg cttcctggaa cgcttcgttg
     2161 atgacgccgg gctgcacagt cgcctgcagg ttgcgctcgt cgatctccag gatgcggttg
     2221 aaccgttcca tcgaaaggca gacgccgccg tggacgggaa gtgcgccgcc gctcaggccg
     2281 gttcccgcgc cgcgcggtgt gaccgggatt ttctccgcgt tgcacaattt cagcagggcg
     2341 gaaatttcct ccgctgtccc gggacgaaca acaacggatg gtttgaacaa aaggtcttct
     2401 gtttcatcct gcccgtaacg ctcgagccgt tcttcgtccg tgtgaacgaa atcgtttcct
     2461 gcaatcctgc gcagttctga aataatatcc gcggttatct tcccgtattc catgttgatt
     2521 ggtaaatatg ccggctgatc atctcccgat ggctttattc tgtgcgatga tgccggaaag
     2581 ttcttcttcg ctgaaacctt ttgcacgcag cgctttgatg acaaaatccg ttttttcccg
     2641 cacataagcg tcgatgtcgt gcggatgcgc ggcggcaatt tgttttttca gcgcaccgta
     2701 ttcgtctctc ttcaccgggt tttccctgag gtaatcgcgg aaggcaagat gattcttcag
     2761 cgctgttatg ctgttccggc agacgtacag gttgtgcagc ggccattcac gctgtgtttc
     2821 gccccagggg acaaattcat cccggcgttt aaaaacttca cgttcatcga tgccgagatc
     2881 gccggcgtgg tcgtaaccca ggtcgtcaag aatcgatttt accggcccga aatgctcttt
     2941 ttcatcaatg accaggtcga tatcgatgat cggctttgca ggaagcccgg gcaccgaagt
     3001 gctgccgaca tggtgaatgc cgctgagaaa ttttcccgcc tttatttcaa acaccgtttt
     3061 cagctgcaga aaaatttccg cccactgcgg ggaatattct tcgatcacga tttttgaagg
     3121 tcctggcatg gaaatcaaat gtaacctgcg cctgccggaa acccaagttt catcaccata
     3181 aattcccgcc ggtcagggct caccgccgct tgcggcggaa ccggattcat tcacaggtct
     3241 tttcct
//
LOCUS       DASTSF010000339         2906 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1581965,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000339 DASTSF010000000
VERSION     DASTSF010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2906
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1581965"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..524)
                     /locus_tag="VFU15_09625"
     CDS             complement(<1..524)
                     /locus_tag="VFU15_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016093067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718083.1"
                     /translation="MAPINRLPVRATLNGGKKNPAFFRVPVFVVFFLSFFLPPAIAQN
                     NVGIGTTTPDPTSILDMQSTTQGVLVPRLTTAQRLAIVSPANGLLVYDTNFDCFFYYI
                     AATTSWQNMCTSSAATGPTGPTGPAGTNGVAGATGATGVAGTTGPTGPAGVAGTTGAQ
                     GPSGIDGVTGPMGVT"
     gene            660..2180
                     /locus_tag="VFU15_09630"
     CDS             660..2180
                     /locus_tag="VFU15_09630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718084.1"
                     /translation="MNASDDLFRLIHSLSKNEKGYFKKSAGRHVKGNGNNYIRLFDAI
                     EKQNVYDEEKVKRKFSGETFIRHLPSEKNYLYHLILRSLREYHSGSSADARMHQDLHD
                     IELLFDKGLFTQCARLLARVKKHAVKFSKPLLSLHIMEWESRILAAQGKLDELEALLR
                     RSGSERKKHIAEFENTSAFRELEDTMFLVSRKYGFPRTKKDRQRYEKVMKHPLMKNEK
                     NASSYPARLHYYNIHNFYFEATGDAKETYRYRKMLVDFAETDEEQMKDNQYRYVVALN
                     NLMNSQDELGLKKDCDRTIAKLQHFPARSMNIRSRIFGYTFNILFTQCILSGQFEKVI
                     AHEAEVEEGLHLYGKLLHPEFHLAFKYQFFYGSFGAGKFRKSLTWMNRLLEDETLRVR
                     PEVYFFARIMNLIVHFELKNTDFLPYLIRSTYRMLIRQKRLFRFENVVLDFIRKSGNY
                     TTAAEMKSAFLRLKTQLEELNKDPYEKQVLSFFDIIAWLESKITRRSFGEIVRSKN"
     gene            complement(2191..2616)
                     /locus_tag="VFU15_09635"
     CDS             complement(2191..2616)
                     /locus_tag="VFU15_09635"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEU4718085.1"
                     /translation="MEKRYPTVFATVDVAILKDGKLLLGRKPHQDKFRFIGGFSDPAF
                     DNSYEDAAIREAKEETQLTVRSVRYLGSARVDDPRYRGTPDCIITHLFVTEEWEGNPQ
                     ASDDIAELRWFDVSSLSENDFVKEHDVLWKMFRARTDVK"
     gene            complement(2625..>2906)
                     /locus_tag="VFU15_09640"
     CDS             complement(2625..>2906)
                     /locus_tag="VFU15_09640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718086.1"
                     /translation="DKTTDYESLYAGYRLDIAKDNTYKLYYKLFNAIDHNEEGAWVFS
                     GDKKDVIFQETGSNTTSDWRILKLKQKELWGKYTDTNGNELEIHLVPLL"
BASE COUNT          734 a          782 c          741 g          649 t
ORIGIN      
        1 gtaacaccca tcggtccggt tacgccatca atgccggaag gaccttgcgc acccgttgtt
       61 ccggccacac cggccggacc tgtgggacct gtagtgccgg caacgccagt ggctcctgtg
      121 gcgccggcaa ctccgttggt acccgcaggt ccggtcgggc ccgttgggcc ggtggctgcg
      181 gaggaagtgc acatgttctg ccacgaagtc gtcgcagcga tgtaataaaa aaagcaatcg
      241 aagttggtat cgtaaacgag aaggccgttc gcaggagaca cgatagccag gcgctgcgcc
      301 gtcgtaagtc gcggcacgag cacaccctgc gttgtcgact gcatatcgag gatggacgta
      361 ggatcaggtg ttgtcgttcc gatgccgacg ttattctggg cgatggccgg cggaagaaaa
      421 aacgagagga agaagaccac gaaaaccggc acgcgaaaaa aagcaggatt cttttttccg
      481 ccgttgagcg ttgcgcgcac cgggagtctg tttatcggag ccatcaggtt gaaaaggatg
      541 gccaaaacta ttcagcactt tattaaagag cgatccagaa ttaaaatatt cttggatttt
      601 gatcatagtt attacaagta catttattta gtattttgtt aacaaaacct tggctttcga
      661 tgaatgcttc cgatgatctt ttccgcctga tccattccct ttcgaagaat gaaaaaggct
      721 atttcaaaaa atccgcgggc cgccacgtga aagggaacgg caacaattac atccgcctgt
      781 tcgacgcgat cgaaaaacaa aacgtttacg acgaagaaaa agtgaaacgg aaattctccg
      841 gcgaaacgtt catccgtcat cttccgtcgg aaaaaaatta tctctaccac ctcattctcc
      901 gttccctgcg tgaataccat tccggctcat cggcggatgc gcgcatgcac caggaccttc
      961 acgacatcga gctgctgttc gacaagggac ttttcacgca atgcgcgcgc ctgctcgcgc
     1021 gggtgaaaaa acacgccgtg aaattttcga agcccctgct ctcgcttcac atcatggaat
     1081 gggaatcgcg gatactggcg gcgcagggaa agctggacga gctcgaagcg ctgctgcgcc
     1141 gcagcggatc cgaacgtaaa aagcacatcg cggaattcga aaacacctct gcgttccgcg
     1201 agctggaaga cacgatgttc ctcgtctcca ggaaatacgg atttccccgc acgaaaaagg
     1261 accggcagcg gtatgaaaaa gtgatgaagc atccgctgat gaaaaatgaa aagaacgctt
     1321 cgagctaccc ggcacgcctg cattattaca acatccacaa cttttacttc gaagccaccg
     1381 gcgatgcgaa ggaaacctac cgctaccgca aaatgctcgt cgatttcgcg gaaacggacg
     1441 aggagcagat gaaggacaac cagtaccgct atgtcgttgc gctgaacaac ctgatgaact
     1501 cgcaggacga actgggcctg aaaaaagatt gcgaccgcac catcgcgaag ctgcagcact
     1561 ttccggcaag atcgatgaat atccgctcgc gcattttcgg gtataccttc aacatccttt
     1621 tcacacagtg cattctttcg ggccagttcg aaaaagtgat cgcacacgaa gcggaagtgg
     1681 aagaaggact tcacctttac ggcaagctgc ttcacccgga attccacctg gcgttcaaat
     1741 accagttctt ttacggaagc ttcggcgcgg ggaaattccg caaatcgctg acgtggatga
     1801 accgcctgct cgaagatgaa acgctccgcg tgcggccgga agtttatttt ttcgcgcgca
     1861 tcatgaacct catcgtccac ttcgagctga agaacaccga ctttctcccg tacctcatcc
     1921 gctcgaccta ccgcatgctc atccgacaga aacgcctctt ccggttcgag aacgtcgtgc
     1981 tcgatttcat ccgtaaaagc gggaattaca cgacagcggc ggaaatgaaa agcgccttcc
     2041 tccgcctgaa aacgcagctc gaggagctca acaaagaccc gtatgaaaag caggtgctct
     2101 cgttcttcga catcatcgcg tggctggaaa gcaagatcac gcggagaagt ttcggcgaga
     2161 tcgtgcgaag caaaaactga tcgcggaaat ttatttcaca tccgtccgtg cgcggaacat
     2221 cttccacagc acgtcgtgct ctttcacgaa atcattttcc gaaagcgatg aaacatcgaa
     2281 ccagcgcagc tcggcgatat cgtcagacgc ctgcgggttt ccttcccatt cttccgtaac
     2341 gaataaatgc gtgatgatgc agtcgggtgt accgcggtaa cgcggatcgt ccacgcgcgc
     2401 ggaaccgaga tagcggacag atcttaccgt gagctgcgtt tcttccttcg cttcgcgtat
     2461 cgcggcatct tcgtatgaat tatcgaatgc gggatctgaa aagccgccga tgaaccggaa
     2521 tttatcctgg tgcggcttgc ggccgagcag gagcttcccg tctttcaaaa tcgccacatc
     2581 gacggtagca aaaacagtag ggtatctttt ttccatcaga tttttcagag aagcggaacg
     2641 agatgaattt ccagctcatt cccgttcgtg tccgtgtatt ttccccaaag ctctttttgc
     2701 ttcagcttaa ggatgcgcca gtcggacgta gtgttgctcc cggtttcctg gaaaatcacg
     2761 tctttcttat cacccgaaaa aacccaggcg ccttcttcgt tgtggtcgat cgcgttgaac
     2821 agcttgtagt aaagcttgta ggtattgtct ttcgcgatat ccagccggta acctgcgtag
     2881 agcgattcgt aatccgtggt cttgtc
//
LOCUS       DASTSF010000340         4596 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_711394,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000340 DASTSF010000000
VERSION     DASTSF010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4596
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_711394"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1456)
                     /gene="ccsA"
                     /locus_tag="VFU15_09645"
     CDS             complement(<1..1456)
                     /gene="ccsA"
                     /locus_tag="VFU15_09645"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HEU4718087.1"
                     /translation="MKYNGEQLIWGQTGHMFVVIAFVTALFSVFANVMRIYRKDQEEL
                     WKRPARLFYIIHAVSVFGIFITILSMLFAHRFEYQYVWQHSRRDMSMNYVLACLWEGQ
                     EGSTLLWLLWHSVIGLILLRRAKDWEAPVISVVALVQVFLSMMLLGLYAGKVHIGSNL
                     FELMRQKEDNIGLPWTKMPDYLTKIPLFHDGRGLNPLLQNYWMTIHPPTLFLGFALTT
                     VPFAFAIAGLLTGRLTEWQKPALPWAFTGIMVLGTGILMGGAWAYESLSFGGFWAWDP
                     VENASLVPWLVLVGAAHVMLLYRFKGRSLFTTFFLCITAFLLVVYSTFLTKSGILSDT
                     SVHAFTEDGLNEELTLFMGFFLWLAVMLLSMNNIIRILYTGAAAIVLISFQFGYHGAS
                     MLILVLSSVISLITGYKLSFPKEKQEEELWSREFWMFVGSLVLLVSSFQIIFFTSSPV
                     MNKFLKIGPVHSLCLGLYHTLGGDWLKKLSEANMA"
     gene            complement(1485..1880)
                     /locus_tag="VFU15_09650"
     CDS             complement(1485..1880)
                     /locus_tag="VFU15_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014679237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017003 - protein-heme linkage [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HEU4718088.1"
                     /translation="MKKTHIVGIVLIAIAIASMFALLGNSSTYADFKEAAGKTGEEVH
                     VAGTLDKTRPMDYNPEKDPNRFSFYMIDRNGNEHHVTLMKSKPQDFERTQQIVVVGTM
                     DKDEFVAKDILMKCPSKYNDGRMQAEASN"
     gene            complement(1877..2125)
                     /locus_tag="VFU15_09655"
     CDS             complement(1877..2125)
                     /locus_tag="VFU15_09655"
                     /inference="COORDINATES: protein motif:HMM:NF040113.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcmD family protein"
                     /protein_id="HEU4718089.1"
                     /translation="MKNAVQKKNILSAFAVLLSLAAAAQPNKPGIDEELFRSLHGNGS
                     MNVVLTVIGIILAGLFLTLFRMDRKLSKLEKEIKEKKS"
     gene            complement(2174..3271)
                     /gene="ccsA"
                     /locus_tag="VFU15_09660"
     CDS             complement(2174..3271)
                     /gene="ccsA"
                     /locus_tag="VFU15_09660"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HEU4718090.1"
                     /translation="MVIVSWKALANVFKRSYKFFCIVFLLIGFAGGILVPLAPGIISA
                     DSSSLSTFSGPFTVKVFGYNTHFRSAKSSLQGWITNEGKQPVNTCATSVNVIDDTHLE
                     LSFPPVAPLNDPLFGLTVYNDADGRLYMNSAIATTDTVRGGIVPACNPVVKNEKQDHF
                     AFPFRYILYESMRNLFFHVPMWFTMIALLSWAVVNNLRFLRGFDMKYDRMANNAITTG
                     IIFGLLGLITGSLWARVTWRAWWVDDVKLNGTAITMLSYLAYLVLRNSMEDEQKRARI
                     SAIYSIFAYVMMIVLIGVLPRLQGTDSLHPGNGGNPAFSSYDLDNSLRVFFYPAVAGW
                     VLLGVWIYSVRYRMSNLKENLAAAEVKSRIA"
     gene            complement(3287..3949)
                     /locus_tag="VFU15_09665"
     CDS             complement(3287..3949)
                     /locus_tag="VFU15_09665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008510575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HEU4718091.1"
                     /translation="MNLGRQTAALFKREILNEQRQKYALYGMLLYVVSTVFVAKFSFR
                     VIKDVPAWNALFWIILLFAAATGIARSFAQETRGRMLYLYSIADPRAVLLAKLLYNCL
                     LMCFLGFTGLAFYTFLVGFPVGNTGFFLAALFLGCITLASAFTMISAIASKAGNNFTL
                     MAILGFPVVLPVMLAAIRLSKHAADGFGWSGGAAYFGVLLAMNIVVTGLAYLLFPYLW
                     RD"
     gene            4010..4576
                     /locus_tag="VFU15_09670"
     CDS             4010..4576
                     /locus_tag="VFU15_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEU4718092.1"
                     /translation="MKKLLAPAAILFALVSISMQSASTTKTLYDFQARTIDGKDYDFS
                     QLKGKKVLIVNTASLCGNTPQYADLEKLYEKYSPSGKFEILGFPCNQFAGQEPGSNDS
                     IHAFCTRNYGVTFQMMEKIDVKGDNQHPLYKWLTNKSENGVEDAPVKWNFQKFMIDEQ
                     GHYVGHVAPGDKPFCDKIVDWIEGKAGN"
BASE COUNT         1128 a         1209 c         1293 g          966 t
ORIGIN      
        1 gggccatgtt cgcttccgaa agttttttca gccagtcgcc gcccagcgta tgataaagtc
       61 cgaggcacag ggaatgaacc ggcccgatct tcaggaattt attcatgacg ggcgaggacg
      121 tgaagaaaat gatctggaat gaagaaacaa gcagcaccag cgatcccacg aacatccaga
      181 actcgcgcga ccataattcc tcttcctgtt tttctttcgg gaacgatagt ttgtaacccg
      241 tgatgagcga aatgacggac gacaggacaa gaatgagcat cgaggcgccg tggtagccga
      301 actggaagct gatgagcacg atcgcagctg cgccggtata caggatgcgg atgatgttgt
      361 tcatcgacag gagcatcacg gccagccaga ggaagaaccc catgaaaagg gtcagctctt
      421 cgttcagccc gtcttcggtg aacgcatgaa cggaagtgtc gctgaggatc ccgcttttcg
      481 tgaggaaagt ggaatacacg acaagcagga atgcggtgat gcagagaaaa aatgtcgtga
      541 aaagcgatcg tcctttgaaa cggtagagca gcatcacgtg agctgcgccc acgagcacga
      601 gccacggcac cagcgatgcg ttttcaacag ggtcccacgc ccagaatccg ccgaagctca
      661 gtgattcgta cgcccacgca ccgcccataa ggataccggt gccgagcacc atgatgcctg
      721 tgaaggccca gggcagcgca ggtttctgcc attcggtgag tcgccctgtc agtaacccgg
      781 caatggcgaa ggcgaacgga acggtggtca aagcaaaacc caggaaaagt gtcggcggat
      841 ggatcgtcat ccagtaattc tgcagcagcg ggttgagtcc gcgcccgtcg tggaagagcg
      901 ggatcttcgt gaggtaatcg ggcatctttg tccacggcag cccgatatta tcttctttct
      961 gccgcatcag ttcgaacagg ttgctgccga tgtgcacctt gccggcgtaa agtccgagca
     1021 gcatcatcga aagaaaaacc tgcacaagcg caacgaccga gatcacgggc gcttcccagt
     1081 ctttcgcacg gcggagaagg ataagcccga taaccgaatg ccagagcagc cagagcagcg
     1141 tgctgccttc ctgtccttcc cacaggcacg ccagcacgta gttcatcgac atgtcgcggc
     1201 gggaatgctg ccatacgtac tggtattcga agcgatgcgc gaagagcatc gagaggatgg
     1261 tgatgaaaat gccgaaaacg gaaacggcgt gaatgatgta gaaaagacgg gcgggtcttt
     1321 tccacagctc ttcctggtct ttccggtaaa tgcgcatgac gttcgcgaaa acggaaaaca
     1381 gcgccgtcac gaaagcgatc acgacgaaca tgtggccggt ttgtccccag atcagttgct
     1441 cgccgttgta tttcatcagt aggttattgg taaaaaaatg cggatcagtt cgacgcttcc
     1501 gcctgcatgc ggccgtcgtt gtatttcgac gggcatttca tgagaatgtc tttcgccacg
     1561 aactcgtctt tgtccatcgt gcccacgacg acgatctgct gcgtgcgctc gaaatcctgc
     1621 ggcttgcttt tcatgagcgt gacatgatgc tcattcccgt tccggtcgat catgtaaaag
     1681 gaaaaacgat tgggatcttt ttccgggttg taatccatcg gcctggtctt gtcaagcgtt
     1741 cccgcgacgt gaacttcttc gcccgttttg ccggctgctt ctttgaaatc agcgtacgtg
     1801 ctgctgttgc ccagcaatgc gaacatgctg gcgatggcga tggcgatgag cacgatcccg
     1861 acgatgtgtg tctttttcat gattttttct ccttgatttc tttttccagc ttcgacagct
     1921 tgcggtccat gcggaacagc gtgaggaaca atcccgcgag aataataccg atcaccgtca
     1981 gcacgacgtt catcgatccg ttaccgtgca ggctgcggaa aagctcttcg tctatgcccg
     2041 gcttgttcgg ctgtgccgcg gctgccagtg acagcaggac cgcgaaggcg gaaagaatat
     2101 tttttttctg aactgcgttt ttcattcgta tcggccgtga aatgaatttc attgtgttcc
     2161 tggtgcgatc ctgttacgcg atccgtgatt taacttctgc agctgcaaga ttttctttca
     2221 ggttgctcat ccggtagcgg acggaataga tccacacgcc gagcagcacc catcccgcga
     2281 cggcaggata gaaaaatacg cgcagcgaat tgtcgaggtc gtagctgctg aatgcgggat
     2341 ttccgccgtt gccgggatgc agcgaatcgg ttccctgcaa acgcggcagc acgccgatca
     2401 gcacgatcat cattacgtaa gcgaaaatgc tgtagatggc tgaaatgcgc gcccgtttct
     2461 gctcgtcttc catggaatta cgcaggacga gatacgcgag atacgacagc atcgtgatcg
     2521 cggtcccgtt gagcttcacg tcgtccaccc accacgcgcg ccacgtgacg cgcgcccaca
     2581 gcgatcccgt gatcagcccg agcagcccga aaataatccc ggtggtgatg gcgttgttcg
     2641 ccatgcggtc gtatttcatg tcgaacccgc gcaggaaacg gaggttgttc accacggccc
     2701 agctcagcag cgcgatcatc gtgaaccaca tcggtacgtg aaagaaaagg ttgcgcatgg
     2761 attcgtaaag gatgtagcgg aaagggaatg cgaaatgatc ctgcttctca tttttcacca
     2821 cgggattgca ggccgggacg atgccgccgc gtaccgtatc ggtggtggcg atggcgctgt
     2881 tcatgtaaag ccgtccgtcc gcgtcgttgt aaacggtaag accgaacagc ggatcgttca
     2941 gcggggcgac gggcgggaaa gaaagttcga gatgcgtgtc gtcgatcacg ttgaccgaag
     3001 tggcgcatgt attcaccggc tgctttcctt cgttggtgat ccagccctgc agcgaagatt
     3061 tcgcggaacg gaaatgcgtg ttatagccga aaacttttac ggtaaatggg ccggagaagg
     3121 tggaaaggct cgaggaatca gcgctgataa tgcccggcgc caggggaacg aggatgccgc
     3181 ctgcgaagcc gataagaaga aaaacgatgc agaaaaattt gtaggatctt ttaaacacgt
     3241 tcgccagcgc tttccacgaa acaatgacca tttggcaagg cagattttaa tcgcgccaaa
     3301 gatacggaaa tagaaggtag gcaagtcccg ttacgactat attcatcgcc agcagaacgc
     3361 cgaaataggc cgcgcctccc gaccagccga agccgtccgc cgcatgtttg gaaagccgta
     3421 tggcggcgag catgacggga agcaccaccg ggaagccgag gatggccatg agcgtgaaat
     3481 tgttccccgc cttcgatgcg atggcggaaa tcatcgtgaa agcggaagcg agcgtgatgc
     3541 agccgagaaa aagtgcagcc aggaaaaagc ccgtgttccc gaccggaaag cccacgagga
     3601 acgtgtagaa cgcaagcccg gtgaagccga gaaagcacat cagcaggcag ttgtagagca
     3661 gcttcgccag cagcaccgcc cgcggatcgg cgatggaata gaggtagagc atgcgcccgc
     3721 gcgtttcctg cgcgaagctc cgcgcgatgc ccgtcgcggc agcgaaaagc aggatgatcc
     3781 agaacaaggc attccatgcc ggcacatctt tgatcacgcg gaagctgaat ttggccacga
     3841 acacggtgga aacgacataa agcagcatcc cgtacaacgc atatttctgc cgctgctcgt
     3901 tcaggatttc ccgcttgaat aatgcggctg tctggcgccc gagattcatg tgccgcgaag
     3961 ataccaccga atgcgctttt tgctatattt caaaaaatat ttacccgcta tgaaaaaact
     4021 gcttgcacct gccgccatac ttttcgctct tgtttccatt agcatgcaaa gcgcttccac
     4081 cacgaaaacg ctttacgatt tccaggccag gaccatcgac gggaaggatt acgatttttc
     4141 acagctgaaa gggaagaaag tgctgatcgt caatacggct tcgctgtgcg gcaatacgcc
     4201 ccagtatgcc gatctcgaaa agctgtatga aaaatattct ccttccggga agttcgagat
     4261 actcggtttt ccctgcaacc agttcgccgg gcaggagccg ggctccaatg attccatcca
     4321 tgctttctgc acgcgcaatt acggcgtgac gttccagatg atggaaaaga tcgacgtgaa
     4381 aggcgacaac cagcatccgc tttacaaatg gctgacgaat aaatcggaga acggggtgga
     4441 agacgcgccc gtgaaatgga atttccagaa gttcatgatc gacgagcagg gacattacgt
     4501 gggtcacgtc gctcccggcg acaagccttt ctgtgacaag atcgttgact ggatagaagg
     4561 aaaagcgggg aattaattac tccgcttcga gcccgt
//
LOCUS       DASTSF010000341         3191 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1615189,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000341 DASTSF010000000
VERSION     DASTSF010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3191
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1615189"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(63..446)
                     /locus_tag="VFU15_09675"
     CDS             complement(63..446)
                     /locus_tag="VFU15_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU4718093.1"
                     /translation="MDAKSNTLNWFELPVADFERAKKFYETIFGVTLHVMDFGNFRMG
                     LFPSEPGNGKLTGAICFGEWYKPSQDGALIYFNANPDMQSVLDRIEKAGGKIIHPKKQ
                     ISPEYGFMAMFIDTEGNRVALHSQN"
     gene            624..1775
                     /gene="bshA"
                     /locus_tag="VFU15_09680"
     CDS             624..1775
                     /gene="bshA"
                     /locus_tag="VFU15_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014772208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl L-malate synthase
                     BshA"
                     /protein_id="HEU4718094.1"
                     /translation="MKIGIVCYPTFGGSGVVATELGKALAHKGHQVHFITYSQPVRLD
                     TFAENIFYHEVVVSDYPLFEYQPYELVLSSRMVDVARNEKLDLLHVHYAIPHASAAFM
                     AKAILADKGISLPFITTLHGTDITLLGRDASFEPVITFAINHSDAVTAVSKSLQQDTY
                     KYFKVEREVEVIPNFICLDEYGPSLKQCQKHTFAPKGEKILVHVSNFRKVKRVEDVLR
                     VFDKVRKKVPSKLILVGDGPERVNIERLCRELKTCDDIISLGKVLNPAEILAVGDLFI
                     LPSETESFGLAALEAMASGVPVISTNTGGLPEVNVNGVTGYTSDVGDVENMAENAVKI
                     LSDENTLKKFKTAALEHAKEFDMAKILPMYEQLYERVLEEAKAGKVKMV"
     gene            complement(1798..2988)
                     /locus_tag="VFU15_09685"
     CDS             complement(1798..2988)
                     /locus_tag="VFU15_09685"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019986552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HEU4718095.1"
                     /translation="MHSERTQIILSSGKDQSLKRFHPWVFSGAIKKIKGPVNEGDTVD
                     IFSNKDEFLGTGHYGSGSIAVRIFSFEQNVNETGFWNEKVRRAVEYRRNAGLFEKNDT
                     NVFRLIYAEGDGLPGLIVDFYNGVAVLQAHSAGMYRERNNIAAALKNAMGEQLVAVYD
                     KSAESLHRNTEETQITNGYILGDFQGEGEVTENGNRFYIDWIGGQKTGFFIDQRDNRA
                     LLAQYSKGKKVANTFCYTGGFSVYAANAGASEVHSVDSSKKAIALTEKNVELNGIKNH
                     TSFAMDTFDFLKEKDGVYDVIVLDPPAFAKSVSAKHHAVMGYKRLNAEAISKIKPGGI
                     LFTFSCSQVIDRKLFSDTIMSASIVAGRNVRVMHRLSQPADHPVNIFHPEGEYLKGLV
                     LYVE"
BASE COUNT          730 a          816 c          861 g          784 t
ORIGIN      
        1 aaaaacggca gtcttcaatt tgaattttca gaaaacaagt tgccggacaa ccctgctccg
       61 gactaattct gcgaatgcag cgccacacgg tttccttccg tgtcgatgaa catggccatg
      121 aagccatatt cgggcgagat ctgctttttc gggtggatga tctttcctcc cgctttttcg
      181 atgcggtcga gcacggactg catgtcggga tttgcgttga aataaatgag cgccccgtcc
      241 tgcgaaggct tgtaccattc accgaagcag atcgccccgg tgagtttgcc gttgccgggt
      301 tcggaaggga aaagtcccat ccggaaattg ccgaaatcca tcacgtgcag tgtcacgccg
      361 aagatcgttt cgtagaattt tttcgcgcgc tcgaaatccg caacaggaag ttcgaaccag
      421 ttgagtgtat ttgattttgc gtccatggta tgataagtat ttgtatacgc ctgtggttat
      481 tcccccggct tcagaagtgg ttaaattaca aatgatccgt tatctccgca atccccagca
      541 cacgccatta tccgcccgat aaacctctac ctttgcataa ttgtaaatcg taatccgtac
      601 atcgtcattt taaatcgttt accatgaaaa taggcatcgt ttgctatcct acattcggcg
      661 gatccggagt tgtggccacg gagctgggca aggcgcttgc gcacaaaggc caccaggtgc
      721 atttcatcac ctacagccag ccggtgcgtc ttgatacatt cgctgaaaat attttttacc
      781 acgaagttgt cgtttccgat tatcctttgt tcgaatacca gccgtacgag ctcgtgctca
      841 gcagccgcat ggtggatgtg gcgcgcaacg aaaagctcga cctgctgcat gtgcattatg
      901 cgatcccgca cgcgagcgcg gcatttatgg cgaaagcgat cctcgccgac aaaggcattt
      961 cgcttccgtt catcaccacg ctgcacggaa cggacatcac gctgctcggg cgcgacgctt
     1021 ccttcgagcc ggtgatcacc ttcgcgatca accattccga tgcggtgacg gcggtttcca
     1081 aaagcctgca gcaggatacg tacaaatatt tcaaggtgga acgcgaagtg gaagtgatcc
     1141 cgaactttat ctgccttgac gaatatggtc cttcgctgaa gcaatgccag aagcacacgt
     1201 ttgcgccgaa gggagagaag atccttgtgc acgtttcgaa tttcaggaaa gtgaaaaggg
     1261 tggaggatgt gctgcgcgtg ttcgacaagg tccggaaaaa agttccgtcg aagcttattc
     1321 tcgtcggcga cggccctgaa cgtgtcaaca tcgagcgcct gtgccgcgag ctgaaaacct
     1381 gcgacgacat catttcgctg gggaaagtgc tcaaccctgc ggaaattctc gcggtcggcg
     1441 acctgttcat tcttccttcg gaaacggaaa gtttcggtct cgctgcgctt gaagcgatgg
     1501 catccggtgt gccggtcatt tccacgaata ccggcgggtt gcccgaagtg aacgtgaacg
     1561 gcgtgacggg ttatacgagc gatgtcggcg acgtggagaa catggcggag aacgcggtga
     1621 agattctttc agatgagaat acgctgaaga agttcaaaac cgctgcactg gagcatgcga
     1681 aggaattcga catggcgaaa atattgccga tgtacgagca gctctacgaa cgtgtgctcg
     1741 aggaggcgaa ggccgggaaa gtgaagatgg tctgacgcgc ggaaaatcag gaggccatta
     1801 ctcgacataa agcacaagcc ctttcaggta ttctccttcg ggatggaaaa tgttcaccgg
     1861 atggtcggcg ggctgtgaaa gacgatgcat caccctcacg ttcctcccgg ccacgatgga
     1921 cgcggacatg atcgtgtcgg aaaataattt ccggtcgatc acctgcgagc aggagaacgt
     1981 gaagagaatt ccacccggtt ttattttaga gatggcttcc gcgttcagcc gtttgtagcc
     2041 catcacggca tggtgttttg cgctgaccga tttcgcaaat gccggcgggt cgagcacgat
     2101 cacgtcgtaa acaccgtcct tctccttgag gaaatcgaat gtgtccatcg cgaacgacgt
     2161 gtggtttttt attccgttca gctccacgtt cttttccgtg agtgcgatcg ctttcttcga
     2221 ggaatcgaca ctgtgtactt ccgaggcgcc cgcattggcg gcgtaaacgg aaaatcctcc
     2281 tgtgtagcag aaggtgttgg ccactttttt tcctttgctg tactgcgcca gcagcgcacg
     2341 gttgtcgcgc tggtcgatga aaaagccggt tttctgtccg ccgatccaat cgatgtaaaa
     2401 gcgattcccg ttctccgtca cttcgccttc accctggaaa tcgccgagga tgtagccgtt
     2461 ggtgatctgc gtttcttccg tattgcggtg aagcgattcg gcacttttgt cgtacactgc
     2521 gacgagctgt tcacccatcg catttttcag cgctgcggca atgttgttcc gttcccggta
     2581 catgcctgcc gaatgcgcct gcagcacggc cacgccgttg tagaagtcga cgatgagccc
     2641 gggcaatccg tctccttctg cataaatgag gcggaaaacg ttggtgtcgt ttttttcgaa
     2701 cagcccggca ttgcgccggt attcgaccgc gcggcgcact ttttcgttcc agaaacccgt
     2761 ttcgtttacg ttctgctcaa aggaaaaaat gcgcacggcg atagatccgc ttccgtaatg
     2821 acccgtgccc aggaattcgt ccttattgga aaaaatgtca accgtgtcgc cttcgttcac
     2881 gggacctttt atttttttga tcgcgccgga aaaaacccag ggatggaaac gtttcagcga
     2941 ctggtctttg cccgagctga ggatgatctg cgtacgttcg gaatgcatgg gcgcaaaggt
     3001 aacgtgataa ttaatattcg gcttgttgtg tttcggaatc tcacattata aaacgaatag
     3061 aaaagaagac agcgccatag ctggttttcc gcagaattcg agttgcagaa aagcagcgga
     3121 ggaatgtgcg taagcaaccc cggaagtgaa gcgcagcgga acggcttggg ttgcgggcgc
     3181 ttttcgaaac g
//
LOCUS       DASTSF010000342         5353 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1307453,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000342 DASTSF010000000
VERSION     DASTSF010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5353)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5353
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1307453"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(131..754)
                     /locus_tag="VFU15_09690"
     CDS             complement(131..754)
                     /locus_tag="VFU15_09690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718096.1"
                     /translation="MKTKYVFHSLLTGFTFVLFSLPLAAGPRTFIHIHDISMNTSLSG
                     NGFGAVNHPSAGIGFGRRLVVSAGPVFRAGDNSNNGMRADLHYALMREEESWYGHSSI
                     YSLISFTHFSDQGLGRAAVRQEENTLCPAAREQVPHFADLRYSGEEYAAGLGFSYRFD
                     FGLFIRSEAGIAYYQTKQKSPLPVPTYRCDNALVLKLGCGIGWAFSR"
     gene            complement(789..929)
                     /locus_tag="VFU15_09695"
     CDS             complement(789..929)
                     /locus_tag="VFU15_09695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718097.1"
                     /translation="MKAPLPFHRRFARVLVKATTPELLIFAFSMMTIFALVVAIFFGL
                     RS"
     gene            1213..2544
                     /locus_tag="VFU15_09700"
     CDS             1213..2544
                     /locus_tag="VFU15_09700"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEU4718098.1"
                     /translation="MKKHITGFFCALSFSAFTFLPATLPAQVWSAMNSGLDSTVNHLA
                     FYHGQLVAGGVFENSGSTPAAHVAWWDGAAWQSFPQSPDGVVNTLLEYNGELYAGGSF
                     SNAGGRTCLNIARWNGTAWDSVGGGITGTAVFSLGVYNGHLIAGGHFTMAGNTPASDI
                     AEWDGSSWTALGSGVSGGTTDGVDALLWYGTDLIAGGHFTQAGTVAAHNIALWDGNAW
                     SAMNAGFNTDVQCLCNFNGQLLAGGTFTSSGLTPVKRLGKWNGNAWVAAAGSQGADNA
                     VTSMTTYGGKLFLCGNFSIIDMVAASRIVMYDGSNFMPMGLGLNAQGFSVAADDGIVY
                     AGGNFTQANTLTVAHVAKWSAVGVGIGEFPGAVFTLFPNPSDGICFVSAGENETPFDI
                     TVYNMSGEIVESISDISREGTIDLTAQPAGIYFYRVTVRGKESGTGRIIRQ"
     gene            2867..4099
                     /locus_tag="VFU15_09705"
     CDS             2867..4099
                     /locus_tag="VFU15_09705"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEU4718099.1"
                     /translation="MKKLYIIVAAILNGAAAYSQCSVVVTQHNNVSCFGSCDGSAQVT
                     TVGVPNFTYVWSPGGQTVQNPTTLCPGTHTVTMTDGNSCQATATVTITEPADISLSAS
                     FNNVTCNGSCNGQATITPSGGTTPYSYSWNDPQSSTTDTANGLCPGSYTCTVTDFNGC
                     SKFTAVTITEPTAMVVTVSTTNATCHGACDGTTMASASGGSPGYTYAWSSGCNTASCN
                     NLCAGSYTLTTTDANSCTHMDTVMISEPTQVMPNATSTNASCPSCSDGTASTAASGGT
                     APYTYMWSPGGQTTSSVTGLAPGTYVVCVTDANLCSDCDTVTVSALTGINEAGEDKSV
                     TLFPNPASSYVWLSLNTTAFVQVNIRDLSGRTIYSEAYSAKAGTPEKIDLTGIPAGPY
                     FITITSGDQTVTRKIIRY"
     gene            complement(4272..4670)
                     /locus_tag="VFU15_09710"
     CDS             complement(4272..4670)
                     /locus_tag="VFU15_09710"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU4718100.1"
                     /translation="MNRFSVFLIDDSAEDTELTLRTLRGSPHISNIFSFRDAEEALDT
                     LLVHPGNGNFPGLIFLDMNMPRMNGLEFVRRIKAQERTKDIPVAILTSSTELPDIRET
                     MKLGVKHFIAKPIDEEDIENIVTEIGNREN"
     gene            5014..>5353
                     /locus_tag="VFU15_09715"
     CDS             5014..>5353
                     /locus_tag="VFU15_09715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718101.1"
                     /translation="MKNLAGFVSFIRDHRLEAVARESMRVARGMDIPLLRFFEHIPDD
                     VLLVQSMKSISDFATSLEDGTYISRQMENLQKWEDDKLDGISKDSIQPTDLVMIYAAQ
                     KKAYYRFLPQY"
BASE COUNT         1283 a         1474 c         1476 g         1120 t
ORIGIN      
        1 cacggcaaag tgacgtacct caagctcgac gatgtgttcg ggtattaaaa aaggaagtcc
       61 gcaggaaaaa tggcgctatc accggcgcca ttttttttgc tgaaagagga accgtctctg
      121 ccacggagcc tcaccgcgaa aacgcccaac ctattccaca acccagtttc agcacgagcg
      181 cattatcaca acggtaagtg ggaacaggca aaggcgattt ttgtttcgtc tggtaatacg
      241 cgatccccgc ttccgagcgg atgaacagcc cgaaatcaaa acgatagcta aacccgaggc
      301 ccgcagcgta ttcttccccg ctgtagcgca ggtctgcaaa atgcggcacc tgctctcttg
      361 ccgccgggca caatgtgttc tcttcctggc gcacagctgc gcggcccagg ccctggtcgg
      421 aaaaatgcgt gaacgaaatg agcgaataaa tgcttgaatg accgtaccag ctttcctctt
      481 cgcgcatcag cgcgtaatgc aggtcagcgc gcatgccgtt gttgctgttg tcgcccgcgc
      541 ggaaaacagg tccggcggaa acaacgagcc ggcggccgaa accgatcccg gcggaaggat
      601 gattcaccgc gccgaagcca ttcccgctga gcgaagtgtt catggaaata tcgtggatat
      661 gaatgaacgt gcgcggacct gccgcaagcg gcagcgaaaa gagcacgaag gtgaaacccg
      721 taagaagcga gtggaagaca tattttgttt tcatggtcgt ggcgttgaat gtgaatgatc
      781 cgctgatctc agctgcgcag tccgaagaaa atggcaacga ccagcgcgaa gatcgtcatc
      841 atggaaaaag cgaaaatgag cagctccggc gtggtcgctt tgacaagaac acgggcgaaa
      901 cggcggtgaa aaggaagggg tgctttcatg gagatacgtt ttatggaaca aaagaagagc
      961 gcccccgtcc cggaatgaaa ccgttatggg aacttccctt tcggatgtaa gattgaagtg
     1021 aaaagtgtgg ttgaggcccg gccagtatat tcaccaggga acgtcgtgcc tgcgcacttc
     1081 gttttccgat cgcaaagaca tgaacgcaca aaaaattccg gcgttaagcg ggggtaacga
     1141 cttgttccta attttacgtt ccgcgaaaac gaagcgtgtg cgccccgttc tctccttcag
     1201 cggaaaacag ctatgaaaaa acacatcacc ggattttttt gcgccctttc tttttccgcg
     1261 ttcacgtttc tcccggcaac cctgcctgcg caggtctggt cggcgatgaa cagcggcctc
     1321 gattccacgg tgaatcacct ggcgttctac cacgggcagc tggtggccgg cggtgttttt
     1381 gaaaattcgg gaagcacccc ggcagcgcac gttgcgtggt gggacggcgc cgcgtggcag
     1441 tcgttcccgc aatcgcccga cggcgtagtg aacacattgc tggaatacaa cggcgagctg
     1501 tatgcgggcg gttcgttcag caatgcgggc ggaaggacat gcctcaacat cgcgcggtgg
     1561 aacggaacgg cgtgggattc ggtcggcggc ggaattaccg ggacagccgt tttttcattg
     1621 ggcgtttaca acgggcatct tatcgccggc ggacatttca ccatggcggg aaatacgccc
     1681 gcttccgata tcgccgaatg ggacggcagc agctggacag cattgggcag cggtgtcagc
     1741 ggaggaacca cggacggcgt ggacgcgctg ctctggtacg gaacggatct tatcgcgggc
     1801 ggacatttca cgcaggccgg aacagtcgcc gcgcacaaca ttgccctgtg ggatggaaac
     1861 gcatggtcgg ccatgaacgc aggcttcaac accgatgtgc agtgcctgtg caatttcaac
     1921 gggcagctcc tcgcgggcgg gaccttcacc tcttcgggac ttactcccgt gaagcgtctc
     1981 ggtaaatgga acggcaacgc gtgggtggcg gccgcaggaa gccagggcgc cgacaacgcc
     2041 gttacgtcga tgaccacgta cggcggaaaa ctttttctct gcggaaattt ttccatcatc
     2101 gacatggtgg cggcatcgcg catcgtgatg tacgacggaa gcaatttcat gccgatggga
     2161 ctcgggctga acgcacaggg attttctgtc gcagccgatg acggcatcgt gtacgcggga
     2221 ggaaatttca cgcaggcgaa cacgctcact gttgcgcacg tcgcgaaatg gtcggcggtt
     2281 ggcgtcggga tcggggaatt tcctggcgcg gtctttacat tgttcccgaa tccttcggat
     2341 gggatctgtt tcgtttccgc cggagaaaat gaaacgccgt tcgatatcac cgtttacaac
     2401 atgagcggag agatcgtgga aagcatcagc gatatttcgc gtgaaggaac cattgatctt
     2461 accgcacaac ctgcgggaat ttatttttac cgtgtaacgg ttcgcggaaa agaaagcgga
     2521 acagggcgca tcatccggca atgaccattt acagtttccc gggttcttcc cctgcagaat
     2581 ttcagctgca gccgggccgg aaagccggca actctttttt tgatccccgc tttttttctt
     2641 ttcattttcc gtttccgggg aaacccgcgg gtgccgcaaa agatcgaaag attaaaacca
     2701 accgtcgaat tcgggcaacc ggagccttct gcgtgcgtta accttgtcca agaggcaagc
     2761 ataccatctt ccacaaaaca aaacgggaat attcctgaat gcacaagcgc gctgtgcaga
     2821 aagaagattg ccgtaaacgg aagtttcatt ctaaaaactc agttttatga aaaagttgta
     2881 catcatcgtc gcagcgatcc tcaacggcgc cgccgcctac agccagtgtt ccgtggttgt
     2941 gacacaacac aacaatgttt cctgcttcgg cagctgtgac ggaagcgcac aggtgacgac
     3001 cgtaggcgtt ccgaacttca cgtacgtgtg gtcgccgggc ggacaaacgg tgcagaaccc
     3061 gacgactctt tgtcccggca cacatacggt gaccatgaca gacggtaatt cctgccaggc
     3121 gacggcaacg gtcacgatca ccgaaccggc cgatatttcg ctcagcgcct ccttcaataa
     3181 cgtcacgtgc aatggttcct gcaacggcca ggcaacgatc acgccttccg gtggaacgac
     3241 gccgtattcc tattcgtgga acgacccgca aagctcgacg acggataccg cgaacggtct
     3301 ctgtcccggc tcgtacacct gcacggtcac cgatttcaac ggctgctcga agttcaccgc
     3361 ggtgacgatc accgaaccca ccgcgatggt agtcactgtt tccacaacga acgcaacctg
     3421 ccacggcgca tgcgacggaa caacgatggc ctccgcatcc ggcgggagcc cggggtatac
     3481 gtatgcgtgg agttccggct gcaacacggc gagctgcaac aatctctgcg ccggaagcta
     3541 tacgctgacg accaccgatg cgaacagctg cacgcacatg gacacggtga tgatcagcga
     3601 accgacgcag gtgatgccga acgccacatc gacgaacgcc agctgtccct cctgctccga
     3661 cggaacagcg tcaaccgcag cttcgggagg cacggcgcct tacacataca tgtggtcacc
     3721 cggcggacaa acaacttcgt cggtcacagg acttgcgccg ggaacgtatg tcgtgtgcgt
     3781 cacagacgcc aatctctgca gcgactgcga cacggtaacc gtttccgcac tgaccgggat
     3841 aaatgaagca ggagaagaca agagcgtaac gcttttcccg aacccggcct cttcgtacgt
     3901 gtggttgtcg ttaaacacca ccgcgttcgt ccaggtgaac atccgggatc tcagcgggcg
     3961 cacgatatac agcgaagcgt attccgcgaa agccggcacg ccggagaaga tcgacctcac
     4021 cggaattccc gcaggaccgt atttcatcac catcaccagc ggcgaccaaa ccgtaacgcg
     4081 gaagatcatc cgttactgaa taaaataaca cttcattccc ggctgccgca ccggggaacg
     4141 gcaggcagaa tgagcgatca ttctcctgtc gtttttttat tgaccggatg gtaaagcaga
     4201 aggcacgcgc ttccggcggc tctttcaaaa cggaaaagaa tgtggtccga aaatttgccg
     4261 tgaagaacgg atcagttctc ccggttcccg atctccgtta cgatgttttc aatgtcctct
     4321 tcatcgatcg gcttcgcgat aaaatgtttc acgccgagtt tcatcgtttc gcggatgtcc
     4381 ggcagttcgg tggaggaagt gaggatcgcc acgggaatat ccttcgtgcg ttcctgcgct
     4441 tttatccggc gcacgaattc gagcccgttc atgcgcggca tattcatgtc gagaaaaata
     4501 agcccgggaa aattcccgtt gcccgggtgc accagcagcg tatcgagcgc ttcttcggcg
     4561 tcacggaaag aaaaaatatt gctgatgtgg ggactcccgc gaagcgtacg cagggtaagc
     4621 tcggtatcct ccgcagaatc gtcgatcagg aaaacggaaa aacggttcat tgcactattc
     4681 ggggataatt cctgagggtc tttctcagca tttgtatgga tcgctttccc gggacgagaa
     4741 aggaaagcga tggccgaatg taaaaatttg acagtaaata cgggcttttg cagtgcaaaa
     4801 atcagcccgc ttttttcaat tacttatcaa acgtttgtaa ttgactgatg gtttccgccg
     4861 gcgcttccgg cgctgccccg caaagcatcg ggccgatacg accgactatc gccgtattcc
     4921 gcccggaaat agaatgacct tgaagaaacc cggcgaacct gcgtaaaatc tggtcaaacc
     4981 atctacttga ttgctggtct ccaatttgtc cgtatgaaaa acctggccgg atttgtctca
     5041 ttcatcaggg atcacaggct tgaagcggtt gcacgtgaaa gtatgcgtgt cgcccgcggg
     5101 atggatattc cgctcctccg ttttttcgag cacatacccg atgatgtgct gcttgttcaa
     5161 tccatgaaat ccatttccga tttcgccaca tcgcttgaag acggaacata catcagccgg
     5221 cagatggaga acctgcagaa atgggaagac gacaagctgg acggcatttc caaggacagc
     5281 atacagccca ccgatctcgt catgatttat gccgcgcaga aaaaagcata ttaccgcttc
     5341 ctgccccagt aca
//
LOCUS       DASTSF010000343         4417 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1358090,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000343 DASTSF010000000
VERSION     DASTSF010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4417
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1358090"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(333..896)
                     /gene="efp"
                     /locus_tag="VFU15_09720"
     CDS             complement(333..896)
                     /gene="efp"
                     /locus_tag="VFU15_09720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014682289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEU4718102.1"
                     /translation="MADTGDVSIGTFLKYNGELVQVVEWQHRTPGNLRAFYQGKMRNV
                     RNGKLVENRFRSGEGVEIVFVEYKNLQFIYAEDSNIVLMDKDTFEQMYLPKEMISSGL
                     EFLKEGMDVKVWFESDQPISAEAPNTVELKVTYTEPGLKGDTATNTLKPATVETGASV
                     NVPLFINDGDLIKIDTRTGAYMERVKQ"
     gene            complement(999..1472)
                     /locus_tag="VFU15_09725"
     CDS             complement(999..1472)
                     /locus_tag="VFU15_09725"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEU4718103.1"
                     /translation="MPKQTAKQSLADVFLVNNRIDLFVLGNIPDKDLGVAHSARSRTI
                     ADQFAHLHNVRIMWLEIHKPALAKTLKKIEKGKATKKLLEESLKKSGDAMADYFRELG
                     SPEKMAKAKKSPYNFFAYVTAHEAHHRGQILLHLKYAGVPFDRDKSYAIWEWEKM"
     gene            complement(1547..3706)
                     /locus_tag="VFU15_09730"
     CDS             complement(1547..3706)
                     /locus_tag="VFU15_09730"
                     /inference="COORDINATES: protein motif:HMM:NF017393.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M43 family zinc metalloprotease"
                     /protein_id="HEU4718104.1"
                     /translation="MNQFLPRIVLSVFLATVFSATVSAQAPALPQQRAAENSYTGNFF
                     TAHPEALKQLRKMQDAARINPHYQPQTTVVIPVVFHVVYKTAAQTIDDSVLLNQLQVL
                     NEDYGKMNADTTIAYNVGYGSLVANTGIQFCLATIDPNSAPTTGIERRNTTTISFTTN
                     DNVKHYSSGGLDIWDPTQYLNVWVCNLSAGLLGYAEFPTGNVSPTDGVVVHYACVGRN
                     NPNGQTVAPFNRGRTLTHELGHWFNLVHMNPDPNCVDNDGIADTPPVSDQLTAWPCCG
                     VLTDNCNPTAPGIMYMNFMFYSDADSSQCMFTPGQVAVMWSTLTNLRSSFLNATTSLC
                     TLPPPDDAGIPSITAPVGNLCNSAISPMVTLRNFGSAALTSCTISYDVDNGPATNYSW
                     SGSLAPGQTAQVTLAAYNATNAQHVFHAWTSSPNGNTDGNTANDADSSTYYFFAGGLS
                     LPFSEGFENMTFPPFGWTVMNPDNSYTWERDTLASSSGMASARVTNFYNTAGQADDLI
                     TPPLDLTSVPNPQFLFEVAYQLYTDPASNPNYSDTLRIFVSTDCGLTWNVAYNNYGTS
                     LVTATPQFSPNEFIPAQPQWRLDSVSLAPWAGCTNALLKIENISGYENDLYIDDLIAN
                     QRIASGVMQMESGIYVNVFPNPSDGNFYADVRLDAPQPMTVTVTDMLGNEVSAQQYGA
                     TSGGRYSIDLRAQPAGVYFVKVSAGDFTEIRRITVAK"
     gene            complement(3758..>4417)
                     /locus_tag="VFU15_09735"
     CDS             complement(3758..>4417)
                     /locus_tag="VFU15_09735"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU4718105.1"
                     /translation="TFQSLFYSYLCPMRVSLQNIGKKFGREWIFRGVDHVFEAGKPTV
                     ILGANGSGKSTLLQVIAGSLIPGEGKTEYVSEGTTVPQEEIYRHVSIASPYLDLVDDF
                     TLHESISFHRQFKNFRNGMKEKDLLEISGLTASKDKQLKNFSSGMKMRVRLLLAILSD
                     TSLLLLDEPCSNLDRQGCEWYAQLVTGNLADRCVIVCSNQVKEEHFFCTEEIRMEAFH
                     Q"
BASE COUNT          999 a         1107 c         1259 g         1052 t
ORIGIN      
        1 cgaagaacac caagcgtttc acgaagggta cgaaggaaaa taaagaaatt tcagttcaaa
       61 gttgggaagt tgaaaggctg ggaggttgga aggtcgggcg aagaatgaca cgcgataagc
      121 taaagtcgtt tttaccacga agaacaccaa gcgtttcacg aagggtacga aggaaaataa
      181 agataattca gttcaaagtt gggaagttgg aaggttggaa agtcggggcg acgaatgacc
      241 cgcgaaatgc gttgacgatt tcaagcccct gaaaaacaaa tcccaaactc gctggtggaa
      301 tttgggatcc gtgtttcatt ccggtaaaac gcttattgct tcacgcgctc catataggcg
      361 ccggtgcgtg tatcgatctt gatcaggtcg ccgtcgttga tgaaaagcgg gacgttgacg
      421 ctcgcgccgg tttcaacggt ggcgggcttc agcgtgttcg tggcggtgtc gcctttgaga
      481 ccgggctcgg tgtacgtcac tttcagctcg accgtattcg gcgcttcggc cgagatcggc
      541 tgatcgcttt cgaaccacac tttcacgtcc atgccttcct tgaggaattc gagtccgctg
      601 ctgatcattt ccttcggcag gtacatctgc tcgaacgtat ccttgtccat gagcacgatg
      661 ttggaatctt ccgcgtaaat gaactggagg ttcttgtatt cgacgaaaac gatctctacg
      721 ccttcgccgg aacggaaccg gttctccacg agctttccgt tgcgcacgtt cctcattttg
      781 ccctggtaaa aggcgcgcag gtttccggga gtgcggtgct gccattcgac aacctgcacg
      841 agttctccgt tgtacttcag aaaagttccg atgcttacgt ctccggtatc agccatggga
      901 attgaatttt aggtgatgaa aatgagttta ttgagggcgc aaatataggg attttcggga
      961 gccggcgaca ggggacctgc agggcaaaat cccgggcctc acatcttttc ccattcccag
     1021 atcgcgtagc ttttgtcgcg gtcaaacgga acaccggcgt atttgagatg gagaagtatc
     1081 tgcccgcggt ggtgcgcttc atgcgcggtg acatacgcga aaaaattgta cggacttttc
     1141 ttcgccttcg ccattttctc cggcgatccg agctcccgga aatagtccgc catcgcatcg
     1201 cctgattttt tcagcgattc ttcgaggagc ttcttcgtcg cttttccttt ttcgatcttc
     1261 ttcagcgttt tcgccagcgc gggtttgtgg atttcgagcc acatgatccg aacgttgtgc
     1321 agatgcgcga actggtcggc gatggtgcgt gaacgtgcgc tgtgtgcaac tccaaggtct
     1381 ttgtccggga tatttccgag gacaaaaaga tcgatgcggt tattgacgag aaatacatcc
     1441 gccagcgatt gttttgctgt ttgtttaggc atcctgttcg tgaaaaagga ttgtcccggt
     1501 ttacggaaca atccctggtt tatacttcag gcaatactac tattcatcat tttgccaccg
     1561 tgatgcgtcg tatttctgta aagtctccgg cggaaacctt cacgaaataa acaccggcag
     1621 gctgtgcacg gagatcgatg gaataacgtc cgccggaagt tgcgccgtat tgctgcgccg
     1681 aaacttcgtt gccgagcatg tcggtaaccg ttaccgtcat cggctgcgga gcgtccagcc
     1741 ggacatcggc gtagaaattt ccgtccgacg ggttcgggaa aacattcacg taaatcccgc
     1801 tttccatctg catgacgccc gaagcaatgc gctggttggc gatgagatcg tcgatgtaga
     1861 gatcgttctc gtaacctgag atgttctcga tcttcagcaa cgcgttcgtg catcctgccc
     1921 acggcgccag cgaaacggaa tccagccgcc actgcggctg cgcaggaatg aattcattcg
     1981 ggctgaattg cggtgtggcc gtcacgaggc tcgttccgta attgttgtac gccacattcc
     2041 acgtcaaacc gcaatcggtg gaaacgaaaa tgcgaagcgt atcggagtaa ttcgggttcg
     2101 atgccggatc cgtatacagc tggtaggcca cttcgaagag gaactgcgga ttcggaacgg
     2161 aagtaagatc gagcggcggc gtgatgagat catccgcctg gcctgccgta ttgtagaaat
     2221 tggtgacgcg cgccgatgcc atgccggagc tggaagcaag cgtatcgcgt tcccacgtgt
     2281 acgaattatc cggattcatc acggtccatc cgaacggcgg gaacgtcata ttctcgaacc
     2341 cttcgctgaa cggaagcgaa agtccgcccg cgaagaaata atacgtggaa gaatcggcgt
     2401 cgttcgccgt gttgccgtcc gtatttccgt tcggactgct cgtccacgca tggaaaacgt
     2461 gttgcgcatt tgtcgcgttg taggccgcga gcgtcacctg cgcggtctgt cccggcgcga
     2521 gcgatcccga ccaggaataa ttcgtagccg ggccattgtc cacatcgtag ctgatcgtgc
     2581 aggacgtgag cgccgccgag ccgaaattgc gcagcgtcac catcggtgaa atggcgctgt
     2641 tgcagagatt tcccacggga gcggtgatgg aaggaatgcc ggcatcgtcg ggcggaggaa
     2701 gcgtgcacag tgatgtcgtt gcgttcagga aagaggaacg gaggttcgtc agcgtggacc
     2761 acatcacggc cacctgcccg ggcgtgaaca tgcactgcga agaatccgca tcggaataga
     2821 acatgaagtt catgtacatg atgccgggcg ctgttggatt gcaattgtcc gtaagcacgc
     2881 cgcagcaggg ccatgccgtc agctgatcgc tcacgggagg cgtatcggcg ataccgtcat
     2941 tgtctacgca gttcggatcg ggattcatgt ggacaagatt gaaccagtga ccgagctcat
     3001 gcgtaagcgt cctgccgcgg ttgaacgggg cgaccgtctg gccgttggga ttgttccttc
     3061 cgacgcaggc ataatgcacc actactccgt ccgtcgggct gacgttgcct gtagggaatt
     3121 ctgcatagcc gaggagcccg gcgctgagat tgcacaccca cacattgagg tattgcgtcg
     3181 ggtcccagat atcgagtccg ccgcttgaat aatgttttac attgtcattc gtggtgaacg
     3241 aaatcgtcgt ggtattcctg cgttcgatgc ccgtggtggg cgcactgttc gggtcgatcg
     3301 tagccaggca gaactggatg ccggtattcg cgacgaggga accgtaaccc acattgtacg
     3361 cgatcgtggt gtcggcgttc attttgccgt aatcttcatt cagcacctga agctggttga
     3421 gcaggacgga atcatcgatg gtctgcgcag cggttttata taccacgtga aaaacgacag
     3481 gaataaccac cgtggtctgc ggctggtaat gcggattgat gcgtgcggca tcctgcattt
     3541 tgcggagctg tttcagcgct tccgggtgtg cggtaaaaaa attgccggta taggaatttt
     3601 cagcagcgcg ctgctgggga agcgcaggtg cctgtgcgct aacggttgcg gagaaaacag
     3661 tggcgagaaa aacggacaaa acaatccggg gtaaaaactg gttcatgctg tattttttaa
     3721 ggaaactaaa agtaggaaag acctgtttga aaagcaatca ttgatggaaa gcctccatgc
     3781 ggatctcttc cgtacagaag aaatgttctt ccttcacctg gttcgaacag acgatgacgc
     3841 agcggtctgc aagatttccg gtgaccagct gcgcatacca ctcacatccc tgccgatcga
     3901 ggttggaaca gggttcgtcc agcaacaaca atgatgtgtc gctcaggatc gcaagcagca
     3961 accgcacgcg cattttcata ccggaggaaa aattcttcag ctgcttgtct ttcgatgcgg
     4021 tgagtcccga gatttcaagc aggtcttttt ccttcatccc gttccggaaa ttcttgaact
     4081 gccggtgaaa cgagatggat tcgtgcagcg tgaaatcatc cacgagatcg agatagggag
     4141 atgcaatgct gacgtggcgg taaatttctt cctgcggcac cgtcgtccct tccgaaacgt
     4201 attctgtttt tccttcgccg gggataaggc ttcccgcgat gacctgcagc agcgtggatt
     4261 tcccggaacc gttcgcaccg agaatgacgg ttggcttacc ggcttcaaag acatgatcga
     4321 ccccgcggaa gatccattca cgcccgaatt ttttaccgat gttctgaagc gaaacgcgca
     4381 tcgggcaaag ataggagtag aatagtgatt ggaaagt
//
LOCUS       DASTSF010000344         6533 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_813565,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000344 DASTSF010000000
VERSION     DASTSF010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 6533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6533
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_813565"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1127
                     /locus_tag="VFU15_09740"
     CDS             <1..1127
                     /locus_tag="VFU15_09740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718106.1"
                     /translation="LLRGGDSMYVFVDVTINPNNSNNPLMVEDSILFETNGNRQHVVL
                     NAVGQDAYFHYYETLSCGEVWMNDKPHVIYGYAIIPPGCDLTVMPGTRVHLHKNAFLA
                     ADSAATLRVLGNSTSPVTFQGDRLEPDYAEEPGQWLFIWLSGGSVNNVIDWAVIKNAS
                     AGIRCDTTGASPNPVLTISNTKIRNMSFAGILGVECTSIDGYNLSISNCAQACVYAAY
                     GGKYRFRHCSFGNYWSIDTRTTPAVVLNNWYKTADEVIIHRDLTQADFYNCILYGPLD
                     TELGLDSAVTPGGQFHYFFSHCVIRTNIDTSDPLHFQQNYINADPAFTDAANSDLHIN
                     GNSSAIDRGDPNVSSSIPYDLDDHLRFVNGIPDIGAYEKQ"
     gene            1199..1666
                     /locus_tag="VFU15_09745"
     CDS             1199..1666
                     /locus_tag="VFU15_09745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718107.1"
                     /translation="MRSIFPFAATAFLLLTVSLFSCTGSNATAALPDRNTRIFDAYLS
                     INFSDSIPSSGRNYLCVTRKTPAREITEQLRTVKKLLRKGKLENWHVIVSSDALVPDS
                     LLPAIPITTDWDGAIDRLDIFQCGLSVVKTSGGKIDQIIPLFPQEEITEKDLE"
     gene            complement(1678..2715)
                     /locus_tag="VFU15_09750"
     CDS             complement(1678..2715)
                     /locus_tag="VFU15_09750"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HEU4718108.1"
                     /translation="MKKLLIFFSLAFPFLAGAQDFHYSMYTMAPLTLNPSLAGSFTGD
                     LRIVNNYRMQWMTVSKPFTTYTFGGDMPLARHDREKPSPDYFAIGANVNVDKAGSTNF
                     KTNQFNGLFSYHKSLDGVGRTFFSFGIQAGVSQRSISLGGQSWDMQFNGLQYDAALPT
                     GENASVGQSFTFFDFGAGASFSSVANDRFKTMGGLAVSHLNRPRVDFMGSDEKQYMKF
                     TLHWSAQVALGQNSNAWLLPQLLFVQEGPARMINAGAGVKYRLSERSHYTNYQSEKSF
                     SIGGMYRVGDAMCGYIRFDVGPVGFAFDYDFNTSQLAVASNGMGALEFMLIYTGIYND
                     KNTRLATPSFY"
     gene            complement(2725..5178)
                     /locus_tag="VFU15_09755"
     CDS             complement(2725..5178)
                     /locus_tag="VFU15_09755"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HEU4718109.1"
                     /translation="MKRELLFRARSVPAFLFFLLLVFFSSHSHAQVFWTEDFGVGCSQ
                     NQQAGSYTGPNGTWTVTQTGTNGTSANDWFISATEAGMGAGNCGSGCLTNGTLTNRTL
                     HVASLSVPLICPTGDCGAAYNAGPSGGNAVTDKRAESPVINCSGQTTITLSFVYMEAA
                     SPPNDQCTVWYFDGTTWSLLSTPGPSNNTGCSGQGKWTAYSTPLPASADNNPNVKIGF
                     HWVNNDDGAGNDPSFAVDDVTLSASASATPPTAAFTASATNVCVGTPVNFTDNSTGGP
                     TGWSWTFPGGTPPSSTSQNVTGVVWNTAGSYTVTLTATNANGSNSTTQVITVNPPPTV
                     TASANPSTVCPGNPSTLTGAGASTYVWNPGNLSGTSVSVSPGATTTYTVIGTSAAGCV
                     DSSTVTVTVQPCNVPQVNFIASDTSICVGDCINFTDLSTNGPTSWSWSFSGASVPSST
                     QQNPTNICYNAAGTYAVTLVATNPNGNGSLTKTGYIIVNPPPTANAGSDVSICTGQQT
                     TLIGSGGSTYLWNPGNHSGSTFTVNPASTTTYTLTVTDANGCTGTDQVVVNVAVCTVP
                     VAAFSANDHQVCEGTCIDFTDNSTGTPTGWTWSFPGAVPSSSTQQNPTNICYNTPGNY
                     NVTLIVTNAFGSDTTVMSNYVSVGAAPNVDAGNYTTIAIGNSTQLNATGGTGTWSWVP
                     SAGLSSPNIPNPLASPTVTTTYTVTLTDAFGCSVSDTVTVDVVEAYSLYLPSAFSPNG
                     DGANDVYYVRGAGIKTLDFIVFDRFGEKVFESTSVNDGWDGTFRGKDCETGIYVYYVK
                     AEFYNATSQEQKGDISLVR"
     gene            complement(5225..6253)
                     /locus_tag="VFU15_09760"
     CDS             complement(5225..6253)
                     /locus_tag="VFU15_09760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA reductase"
                     /protein_id="HEU4718110.1"
                     /translation="MTNEERISAFAALGSEIKRVLSGNPATESGKALLTELSSLYLSN
                     GWFTEENIRHRLSVIAGNLGKNALEKWMSEYAIGEEKRKKIVVIMAGNIPLAGFDDFR
                     CVLLSGNDFTGRLASDDKRLPVLLAGMLVEMEPRFAERIVFHEGILKEADAVIATGSN
                     NSSRYFEHYFSKFPNIIRRNRNSVAVLTGNETEAELKELGEDVFRYFGLGCRNVTKLF
                     VPEKYDFGAFFRAIVDWGEVMCNNKKYMNNYEYHRTLFLLNSEKLLDNNFLLLKQDVG
                     ISSPPGVLYYEFYADKNEVENRLRADRELIQCVVDGNGTAFGCAQLTGLSDFADNIDV
                     MDFLIPLS"
     gene            6316..>6533
                     /locus_tag="VFU15_09765"
     CDS             6316..>6533
                     /locus_tag="VFU15_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018674503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEU4718111.1"
                     /translation="MAIKITDECINCGACEPECPNNAIYEGGAEWRFSDGTAVKGMFN
                     SKSGMTADADAPQEPKSMDVYYIVTDKCT"
BASE COUNT         1539 a         1804 c         1730 g         1460 t
ORIGIN      
        1 tcctgctgcg cggcggtgac agcatgtatg tgtttgtgga tgttacgatc aacccgaaca
       61 actccaacaa cccgctcatg gttgaggatt ccatcctctt cgaaacgaac ggcaaccggc
      121 agcacgtggt gctcaacgcc gtgggacagg acgcttattt ccattattac gaaacactca
      181 gctgcgggga agtgtggatg aacgacaagc cgcacgtgat ctacggttac gcgatcattc
      241 cgcccggctg cgatcttacc gtcatgcccg gaacgcgcgt tcacctgcac aagaacgcgt
      301 tccttgccgc cgattccgcc gctacgctgc gcgtgctggg taattccacg agtccggtca
      361 ccttccaggg ggaccgcctc gaaccggatt acgccgaaga gcccgggcaa tggcttttca
      421 tctggctttc cggcggcagc gtgaacaatg tgatcgactg ggccgtgatc aaaaatgcga
      481 gcgctggcat tcgctgcgat acgacaggag caagtccgaa tcctgtgctc accatcagca
      541 acacgaagat ccgcaacatg tcgtttgcgg gtatcctcgg tgtggaatgc acatcgatcg
      601 acgggtacaa cctttccatt tcgaattgcg cacaggcctg cgtgtatgcc gcgtacggcg
      661 gaaagtaccg tttccgtcac tgctcgttcg gaaattactg gagcatcgat acgcgcacaa
      721 ctccggccgt ggtgctgaac aactggtaca aaactgccga tgaagtgatc attcaccgcg
      781 acctgacgca ggctgatttc tacaactgca ttctgtacgg accgctggat acggagctcg
      841 gtcttgattc agccgttaca ccgggcggac agttccatta tttcttttcg cactgcgtga
      901 tcaggacgaa catcgacacg tctgacccgc tgcatttcca gcagaactac atcaatgcag
      961 acccggcttt cactgacgca gcgaacagcg acctgcacat caacgggaat tcttccgcca
     1021 tcgatcgcgg cgatccgaac gtttcctctt ctattcctta cgatctcgat gatcatctcc
     1081 gtttcgtgaa cggcatcccg gatatcggcg cttatgaaaa gcagtgattt tccgttttca
     1141 aacaattaaa accgatttct tttttcagcg gtgaatatgt tcgcgcaaaa tttaaatcat
     1201 gcgcagcatt tttccatttg ccgccacggc attcctcctg cttaccgtat ccctattttc
     1261 ctgtaccgga agcaatgcga ccgcagcgtt gcccgacagg aatacacgca tcttcgatgc
     1321 ttatctctcg atcaatttca gcgattcgat cccttcgtca ggaagaaatt acctgtgcgt
     1381 tacgcgaaaa acaccggcac gggaaataac agaacagctg cgaacggtaa aaaaactgct
     1441 ccgcaaggga aagcttgaaa actggcatgt cattgtcagc tcggatgcgc tggtgcctga
     1501 ttcgcttctt cccgcaattc ccatcaccac cgactgggac ggcgcgatcg acaggctgga
     1561 tatttttcag tgcgggcttt ccgtcgtgaa aacatccgga ggaaaaatag accagatcat
     1621 cccgcttttc ccgcaagaag agatcaccga aaaagatctt gaatagcgaa gagcggctca
     1681 gtagaagctc ggcgtagcga ggcgcgtgtt cttgtcgttg taaatgccgg tgtaaataag
     1741 catgaactcg agcgcgccca tgccgttgct cgcgaccgca agctgcgacg tgttgaagtc
     1801 gtagtcgaaa gcaaatccca ccgggccgac atcaaagcgt atatagccgc acattgcatc
     1861 gcctacacgg tacattccgc cgatggaaaa agatttttcc gactggtaat tcgtgtaatg
     1921 cgaacgttcg ctcaggcggt atttcactcc tgcgccggcg ttgatcatgc gcgcaggacc
     1981 ttcctggaca aacagcaatt gcggcagcag ccaggcattc gagttctgcc cgagcgcaac
     2041 ctgcgccgac cagtgcaggg tgaatttcat gtattgcttt tcatccgagc ccatgaagtc
     2101 aacgcgcggg cgattaagat gcgaaaccgc cagcccgccc atcgttttga aacggtcgtt
     2161 cgccacgctc gaaaaagaag cgcctgcgcc gaaatcaaaa aacgtgaacg actgcccgac
     2221 agaagcattc tctcccgtgg gaagcgccgc atcgtactgg agcccgttga actgcatgtc
     2281 ccagctttgc ccgccgagcg aaatgctccg ctgcgaaaca ccggcctgga taccgaacga
     2341 gaaaaaagtc cgtcccacac cgtcgagcga tttgtggtag gaaaaaagtc cgttgaactg
     2401 gttcgttttg aaattcgtgc tgccggcttt gtccacgttc acattcgcac cgatcgcgaa
     2461 ataatcgggg ctcggttttt cacggtcatg acgggcaagc ggcatatcgc cgccgaaagt
     2521 atacgtcgta aagggttttg aaaccgtcat ccattgcatg cggtaattgt tcacgatacg
     2581 aaggtctccc gtaaaagaac ccgccagcga tgggttcaat gtcaggggcg ccatcgtgta
     2641 catggaataa tgaaaatcct gcgcaccggc gaggaacggg aatgcaagcg aaaaaaagat
     2701 cagcagcttt ttcatttttc ctccttatct gaccagtgaa atatcacctt tctgttcctg
     2761 gctggtggcg ttatagaatt ccgcttttac gtagtaaacg tagatgccgg tttcgcaatc
     2821 tttgccgcgg aacgttccgt cccagccgtc attcacgctc gtgctttcaa aaactttttc
     2881 cccgaagcgg tcaaagacaa tgaaatcaag cgtttttatt ccggcccccc gaacataata
     2941 gacatcattc gctccatctc cgttcggtga aaatgcagaa ggcaggtaaa gcgaatacgc
     3001 ttccacaaca tccaccgtga cggtgtcgga aacagaacag ccgaatgcgt ccgtcagcgt
     3061 aaccgtgtac gtggtagtaa ccgtaggact tgccagcggg ttcggaatat tcggagaaga
     3121 aagcccggcc gacggcaccc acgaccatgt cccggtcccg cccgttgcat tcagctgcgt
     3181 ggagtttcca atggcaatgg tggtatagtt tccggcatca acattcggag ccgcaccgac
     3241 gctgacgtaa ttgctcatca ctgtcgtatc ggaaccgaac gcattggtca cgatcagcgt
     3301 gacgttgtaa ttcccgggag tgttgtaaca gatgttcgtc gggttttgct gcgttgacga
     3361 agaaggcacc gcaccgggaa agctccaggt ccacccggtc ggcgtgcccg tgctgttgtc
     3421 ggtgaaatca atacaggttc cttcacacac ctggtggtca ttcgcagaaa atgctgcaac
     3481 aggaacggta caaaccgcga cgttcaccac cacctggtcg gtgcccgtgc acccgttcgc
     3541 atccgtaacg gtgagcgtgt atgtggtggt ggacgcagga ttcaccgtga acgtgctgcc
     3601 ggagtgattg cccgggttcc agagataagt ggaacctccg gaaccgatca gcgttgtctg
     3661 ctgcccggtg cagatggaaa cgtcagagcc ggcgttcgcc gtgggcggag gattgacgat
     3721 gatataaccg gttttcgtca gcgatccgtt cccgttcggg ttggtggcga cgagcgtcac
     3781 cgcatacgta ccggcggcgt tgtagcagat attggttgga ttctgctgtg tggacgacgg
     3841 aacgcttgcc ccgctgaacg accagctcca gctcgtcggt ccgttcgtgc tgagatccgt
     3901 aaaattgatg cagtcgccga cgcagatgct ggtgtcgctg gcgatgaaat tcacctgcgg
     3961 aacattgcac ggctgcacgg taactgtaac tgttgacgaa tccacgcaac ccgctgcgct
     4021 cgtaccgata accgtgtacg tggtggtcgc gcccggagag acggaaacag aagttcccga
     4081 aaggttcccc ggattccaga cgtaggtcga ggcgccggca cctgtcagcg tgctcgggtt
     4141 cccggggcac acggtggacg ggttggcgga tgccgtaact gtcggcggcg gattcaccgt
     4201 gatgacctgc gttgtgctgt tgcttccgtt cgcattggtg gctgtcagcg taacggtata
     4261 agaaccggcg gtattccaca cgacgccggt aacgttctgc gaggtggaag acggcggagt
     4321 tccgcctggg aatgtccagc tccagccggt aggaccgccc gtggagttat ccgtaaaatt
     4381 aaccggtgtg ccgacacaga cgtttgtcgc gctcgccgtg aatgccgcag tgggtggcgt
     4441 agcggaagct gaggccgata atgtgacgtc gtccaccgcg aaagatggat cgtttccggc
     4501 gccgtcatcg ttgttcaccc agtgaaaacc gatctttaca ttggggttgt tatccgcaga
     4561 agcaggcaac ggtgtcgagt aagccgtcca ctttccctgg ccactgcatc cggtgttgtt
     4621 cgaaggaccg ggagtagaaa gcagcgacca tgtcgttccg tcgaaatacc agaccgtgca
     4681 ctggtcattc gggggactgg ccgcttccat gtaaacgaaa gaaagtgtga tggtcgtctg
     4741 gcccgagcaa ttgatcaccg gggattccgc acgtttatcg gtgacagcgt ttccgccgga
     4801 aggacctgca ttgtacgcag cgccgcaatc accggtcggg caaatgagcg gaacactcag
     4861 gctggccaca tgcagcgtac ggttggtgag ggtcccattc gtaagacaac cgctcccgca
     4921 atttcccgct cccattcctg cttccgttgc gctgatgaac caatcgtttg ccgaagtccc
     4981 gttcgttccc gtttgggtaa ccgtccaggt tccgttcggc ccggtgtacg aacccgcctg
     5041 ctggttctgg ctgcagccca cgccgaaatc ttccgtccag aaaacctggg catgcgaatg
     5101 cgacgaaaaa aaaacgagaa gcagaaaaaa aagaaatgcg ggaactgatc gcgcgcgaaa
     5161 gagtaattct cttttcatat tcctggattt gccagtccta aatatactga aaatcctttt
     5221 tcgttcacga cagcgggatc agaaaatcca tcacgtcgat attgtcggcg aaatcagata
     5281 agccggtgag ctgggcgcat ccgaaagcag ttccattgcc atccaccacg cactggatca
     5341 attcacggtc ggcccgcagg cgattctcca cctcgttttt atccgcatag aattcgtaat
     5401 acagaacacc gggcggggaa gaaatgccca catcctgctt cagcaaaagg aaattattat
     5461 cgagcagctt ttcactgttg agcaggaaca gcgtgcggtg gtactcgtaa ttgttcatgt
     5521 actttttatt gttgcacatg acttctcccc agtcaacgat cgcgcggaaa aaagcaccga
     5581 aatcatattt ctccggaaca aaaagtttcg tcacgttcct gcagccgagg ccgaaatagc
     5641 ggaacacatc ctcgcccagc tctttcagct ccgcttccgt ttcatttccc gtaagcaccg
     5701 ccacgctgtt ccggttgcgc cggatgatat tgggaaattt cgaaaaataa tgctcgaaat
     5761 agcgcgatga attgttgctc ccggttgcaa tgacggcatc ggcttctttc aggatccctt
     5821 cgtggaaaac gatacgttcc gcaaagcgcg gttccatctc caccagcatg ccagcaagaa
     5881 gaaccgggag acgtttatca tccgaagcca gcctgcctgt gaaatcatta ccggaaagca
     5941 gcacgcagcg gaaatcgtcg aacccggcca gcggaatgtt ccctgccatg atgacgacga
     6001 tcttttttct tttctcctcc ccgatcgcgt attccgacat ccatttttca agcgcatttt
     6061 ttccgagatt cccggcaata acagaaagcc ggtggcggat attctcttcc gtgaaccagc
     6121 cattcgagag ataaagcgag gaaagctccg tgagcaatgc cttaccggac tccgtggcag
     6181 ggtttcccga aagcacacgt ttgatctcgc tccccaatgc ggcaaaggct gaaatccgtt
     6241 cttcgtttgt catttattcg cggacaggaa tgcagatgaa aatgtgtaat tttgcgatgt
     6301 aaaattacga acaagatggc catcaagatc accgacgaat gcattaactg cggagcgtgc
     6361 gaaccggaat gcccgaacaa cgccatttac gaaggcggag ccgaatggag attctccgac
     6421 ggaacggctg taaaaggaat gttcaactcg aaaagcggca tgaccgccga cgctgacgcc
     6481 ccgcaggaac cgaaaagcat ggacgtgtat tacatcgtca ccgacaaatg cac
//
LOCUS       DASTSF010000345         2605 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_735599,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000345 DASTSF010000000
VERSION     DASTSF010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2605
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_735599"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            136..972
                     /locus_tag="VFU15_09770"
     CDS             136..972
                     /locus_tag="VFU15_09770"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012780162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEU4718112.1"
                     /translation="MEEEYNYRRIEKAIQFLSSDFRNQPSLEEIASHCAMSPFHFQRV
                     FTEWAGVSPKKFLQYLTVESLKRELHEKENLFTAAESVGLSSPSRVYDLFVNTESVTP
                     QEFRTGGEGISIAYGFEETPFGKCFIALTGRGVCAMSFTDGNEAETLEEFRGKWPGAQ
                     VREDRAHTKPVAESIFRRGGGKPAEVKLLLKGTPFQLKVWEALLNIPFGKVTVYGRLA
                     SSIGSPSAARAVGTAVGSNPVAFLIPCHRVIRNEGIIGQYRWGSARKTAMLGWERSRM
                     EK"
     gene            1155..1565
                     /locus_tag="VFU15_09775"
     CDS             1155..1565
                     /locus_tag="VFU15_09775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718113.1"
                     /translation="MIKKRICADCGEELNGRADKKFCSDQCRNNFNNRLNSDNNSLIR
                     NINNILKRNRRILSELAPSGKATLHRDRLSDKGFRFGYSTHSYVTRKGATYYFCYDFG
                     YLRLPGDFITIVQRESASKTDTAVAAEAETSAVA"
     gene            1702..>2605
                     /locus_tag="VFU15_09780"
     CDS             1702..>2605
                     /locus_tag="VFU15_09780"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEU4718114.1"
                     /translation="MSSALAAQEAACVSPDVIRVMRVSDSIAHYMVQTTQIYDAGLDT
                     LPQMKFWRKIMNMSPDSGFVSNGLDRTIYAVQPSSWWEKLGKDGQEHYRDSLRKAQGL
                     CDSIPVYFTSGKNDFYNAKSVIPDIDRAIPIFVEEGVDPFYAQAILLIESPGQYRKSP
                     AGAAGSFQLMPGVARQMGLVVNKKTDERKDFDKSAWAAAKLLKSICIPCTNAMLDKRG
                     ICYDENDLWYRLLVLHVYHAGAGNVEKALQVINPTEGGMWLIEKLWQTKVGAFGKSSQ
                     SYSQLVVSAFLELDQALGRNSGDAE"
BASE COUNT          666 a          655 c          696 g          588 t
ORIGIN      
        1 gtccggaccg cactcatcaa cgggtgcaca gaaaaccctt cagaaatgga gggtttttct
       61 ttttccctaa ttttcatttc agcaagttcc gggttgggca ggtcgcagga tgtgctgaat
      121 tttcgaaaaa aaaacatgga agaagaatac aattaccggc gcatcgaaaa agcgatacaa
      181 ttcctgtcgt ctgatttcag gaaccagcct tcgctggaag agattgcatc gcactgcgcc
      241 atgagcccgt tccatttcca gcgtgttttc accgaatggg ccggcgtcag cccgaagaaa
      301 ttcctgcaat acctgaccgt cgagtccctg aaacgtgaac tgcatgaaaa agaaaacctg
      361 ttcaccgctg ccgaaagcgt cggcctttct tcgccttcac gcgtttacga cctgttcgtg
      421 aatacggaat ccgtcacccc gcaggaattc aggacgggcg gggaaggaat ttccattgcc
      481 tatggttttg aagaaacgcc cttcggcaaa tgttttattg ccctgaccgg aagaggcgtc
      541 tgcgccatga gttttaccga cgggaacgaa gcggaaaccc tggaggaatt tcgcggcaaa
      601 tggccgggtg cgcaggtgag ggaagatcgc gcgcacacga aacccgtagc ggaaagcatc
      661 ttccgccgtg gcggcgggaa accggcagaa gtgaaattgc tgttgaaggg aacgccgttc
      721 cagcttaaag tatgggaagc cctgctgaat attcctttcg gtaaagtgac ggtttacgga
      781 agactggcgt cctccatcgg ttcaccttcg gccgcacgtg ccgtgggaac tgcggtgggc
      841 agcaacccgg tcgcatttct tattccctgc caccgcgtga tccgcaacga agggatcatc
      901 gggcagtacc gctggggaag cgcgcggaaa acagcaatgc tcggctggga aagatcaagg
      961 atggagaaat aaaatgtgcg gaaaagcaga aaaacaccgg attcaatccc atcaaatagt
     1021 ataaaaaatg tgagacgatc gtctattgat catgtttgtt tgcccgtaac ggtttgaatt
     1081 ttttgtaaaa aataatgtat aatgttttac atattttaat aaaaaattaa tttcgcggca
     1141 ttaaacccat acacatgatc aaaaaaagaa tttgtgccga ctgtggcgaa gagctcaatg
     1201 gtcgcgctga caaaaagttc tgttccgatc aatgcaggaa caacttcaac aaccggctga
     1261 acagcgacaa caacagcctg atccgcaaca tcaacaacat tctcaaaagg aaccggcgca
     1321 ttctttcgga gctggcgcct tcaggaaaag caacgctcca cagggaccgt ttgagcgaca
     1381 aaggttttcg cttcgggtat tccacgcatt cctacgtgac gcggaaaggc gccacttact
     1441 atttctgcta tgatttcggg tacctccggc tacccggcga ttttattact atcgtgcagc
     1501 gggaatcggc ctcgaagacc gataccgcgg tggcggctga agcagagacc agcgctgtcg
     1561 cctgagaaaa taaacgttaa agcgttgaaa aaaattaccg ggagtgaaac cttttatggg
     1621 tagtctttcg tttctttgaa agaaaggcga cccttgatga tggtgaactc ccggaaattt
     1681 ttcactttct tatttgctgt tgtgtcttcc gcgctcgctg cgcaggaagc ggcatgcgtc
     1741 agtcccgacg tcatccgcgt tatgcgggtg agcgattcga tcgcgcacta catggtgcag
     1801 accacccaga tctatgatgc gggcctggat acacttcccc agatgaaatt ctggcggaag
     1861 atcatgaaca tgtcgccgga ttccgggttt gtgagcaacg gcctcgatcg cacgatctat
     1921 gccgtgcagc cttcctcctg gtgggaaaag ctggggaagg acgggcagga acattaccgc
     1981 gacagtctcc gtaaagcaca gggcctctgc gacagcattc ctgtgtattt tacctccggt
     2041 aaaaatgatt tctacaacgc aaaatcagtt attcccgaca tcgacagggc catcccgatt
     2101 tttgtggaag aaggagtgga cccgttctac gcgcaggcca tcctgctgat cgaaagtccg
     2161 gggcaatacc gtaaatcacc tgcgggggct gccggctctt tccagctcat gccgggcgtg
     2221 gcaagacaga tgggactcgt ggtgaacaaa aagaccgatg agcgcaagga ttttgacaaa
     2281 tcggcctggg cggctgccaa gctgctgaaa agtatctgca tcccctgtac gaacgccatg
     2341 cttgacaagc gcggaatatg ttacgatgaa aatgacctct ggtaccggtt gctcgtgctg
     2401 catgtttatc atgcaggagc cggcaatgtc gaaaaggcgc tgcaggtaat taatcccacc
     2461 gaaggcggca tgtggctgat cgaaaagctc tggcagacca aggtcggtgc gttcgggaaa
     2521 tcgtctcaat cctattccca gctggtcgtt tccgctttcc tcgagctcga ccaggcgctg
     2581 ggccgcaatt ccggcgatgc ggagc
//
LOCUS       DASTSF010000346         2425 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_948249,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000346 DASTSF010000000
VERSION     DASTSF010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2425
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_948249"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..324)
                     /locus_tag="VFU15_09785"
     CDS             complement(<1..324)
                     /locus_tag="VFU15_09785"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU4718115.1"
                     /translation="MNQLLQGKVIVVTGGAGLLGKAFCSAICESGGTPIVADKNEKDG
                     RAFVEELNKKYGRRAHYFPLDITSESSIETMIETVHNEFGYIDALVNNAYPRNKNYGR
                     VFEEVT"
     gene            complement(321..1055)
                     /locus_tag="VFU15_09790"
     CDS             complement(321..1055)
                     /locus_tag="VFU15_09790"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU4718116.1"
                     /translation="MPEEKGSDYIKALQLGKEGAGEYYRKSVVKTEQQKFLEQLIKEK
                     KLSCTKIADVACGGGSLTFHLRTIFPGASFSLCDFNPDALEIAKELNGKECSYSVENI
                     YSLSFPDNTFDLVCCWQTLSWLDDPQTALNELVRIAAPGGTIYVSSLFNIGHDVDIYA
                     KLTDHTRPEGQAGMSYSYNTYSQRSVEKWLDGKVNAWKLHEFTPSVDFSYEGRGLGTF
                     TVNSEHKRLQVSGGYLMNWAILEIEK"
     gene            complement(1058..2359)
                     /locus_tag="VFU15_09795"
     CDS             complement(1058..2359)
                     /locus_tag="VFU15_09795"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005466255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HEU4718117.1"
                     /translation="MSKNDYSKRLHHVIPGGAHTYSRGDDQYPLNAPPILSRGKGAYV
                     WDPDGNKFLDYGMALRAVTLGYDYEPVSEAAIAEIRKGNNLTRATVTELEAAELLTSL
                     FKGADMVKFAKNGSTVTTAAVKLARAYTGRKFVAVCAEHPFFTYDDWFIGVTPMDKGI
                     PEEHRSLTLTFHYNDPASLQQLFDQHPGQIAAVMLEPASTVQPKDNFLHKVKDICHRN
                     GAVFILDEMITGFRWDLHGAQKHFGIQPDLCTFGKGMANGFSLAALAGKREIMSLGGI
                     LEEGQERVFLISTTHGAEMCALGAFIKTVEVYRSENVTDHLWKYGSALMDGMNGIAKA
                     LGIEKEFSAEGFPCSPYYMTRDRSGNASLEMRTLFSQEMIRNGVLMPWIALSLAHGTL
                     ELEMTLNAVERALKVYRDALEKGAGTYLEGRPIRPVFRKHN"
BASE COUNT          502 a          685 c          639 g          599 t
ORIGIN      
        1 cgtcacctct tcgaaaacgc gcccgtaatt tttattgcgc gggtaggcgt tgttcaccag
       61 cgcatcgatg tagccgaact cgttgtgcac ggtttcgatc atcgtctcaa tgctggattc
      121 cgaagtgatg tcgagcggaa aataatgcgc ccgtcttccg tattttttat tgagctcttc
      181 gacgaatgcg cgcccgtcct tctcgttctt atccgcgacg atcggtgtgc cgcccgattc
      241 gcagatcgcg gagcagaaag cttttcccag caggcccgcg ccgccggtga caacaataac
      301 ttttccctgg agaagctgat tcatttttca atttcgagta tggcccagtt catcaggtat
      361 ccgcccgaga cctggaggcg tttgtgttcc gaatttacag taaatgtgcc caatccccgt
      421 ccttcatagc tgaagtcgac agaaggagtg aattcgtgga gcttccatgc gttcactttt
      481 ccgtccagcc atttttccac ggacctctgc gaataggtat tgtacgaata cgacatcccc
      541 gcctgtcctt cgggccgcgt atgatcggta agctttgcat aaatatcgac gtcatggccg
      601 atgttgaaaa gcgagctcac atagatcgtt ccgccgggag cggcaatgcg caccagctca
      661 ttcaacgcgg tttgcggatc atcgagccag gaaagcgtct gccagcagca gacaagatcg
      721 aatgtgttgt ccgggaacga gagcgaataa atattctcca ccgagtagct gcattccttt
      781 ccgttcagct ctttcgcgat ctccagcgca tcgggattga aatcgcagag cgaaaaggat
      841 gcgccgggga aaatagtacg gagatggaac gtcagcgaac cgccgccgca ggcgacatcg
      901 gcaattttcg tgcacgacag ttttttttcc ttgatcagct gctcgagaaa tttctgctgc
      961 tcggttttca ccaccgactt gcggtaatat tctcccgcgc cttcttttcc cagctgcagt
     1021 gcctttatat aatcgctgcc tttttcttcc ggcatgttca gttgtgtttg cggaataccg
     1081 gcctgatcgg gcggccttcg agatacgtgc cggctccttt ttcaagcgcg tcgcggtaaa
     1141 ctttaagcgc gcgttccacc gcgttcagcg tcatttccag ttccagcgtt ccgtgcgcaa
     1201 gcgagagcgc gatccacggc atcagcacac cgttgcggat catttcctgt gaaaacagcg
     1261 tacgcatttc cagcgatgca ttgccggaac ggtcacgggt catataatac ggtgaacagg
     1321 gaaaaccttc cgcggaaaat tctttttcaa tgccgagcgc tttcgcaatg ccgttcatcc
     1381 cgtccatcag cgcgcttccg tatttccaca aatggtccgt cacgttctca ctgcgataaa
     1441 cttccaccgt tttgatgaac gcaccgagcg cgcacatttc cgcaccgtgt gtcgtggaaa
     1501 tgaggaacac gcgttcctgt ccttcttcga gaattccccc gagcgacatg atctcgcgtt
     1561 tccccgcaag cgccgcgagg gaaaaaccgt tcgccatccc tttaccgaac gtgcagagat
     1621 ccggctgaat tccgaagtgc ttctgcgcgc cgtgcaggtc ccagcggaaa cctgtgatca
     1681 tttcatcaag aatgaaaacc gcgccgttgc ggtgacagat gtccttcacc ttgtgcagga
     1741 aattatcttt cggctgaacc gtcgatgccg gctcgagcat cacggcggct atttgtcccg
     1801 ggtgctgatc gaataactgc tgcagggaag ccgggtcgtt gtaatggaat gtgagcgtaa
     1861 gcgagcggtg ctcctccgga atacccttat ccatcggtgt gacgccgatg aaccagtcgt
     1921 cgtacgtgaa gaacggatgt tccgcacaca cggccacgaa ttttcttcct gtgtacgcac
     1981 gggcaagctt cacagcggcg gtagtaaccg tcgaaccgtt cttcgcgaac ttcaccatgt
     2041 cggcgccttt gaacagcgag gtcagcagtt ctgccgcctc cagctccgtc actgttgcgc
     2101 gcgtgaggtt gttccctttg cggatctcag cgatcgccgc ctcggaaaca ggttcataat
     2161 cgtagccgag cgtgacggcg cgcagcgcca tgccgtaatc gagaaattta ttgccgtccg
     2221 gatcccaaac gtacgcgcct ttcccgcggc tgagaatggg cggtgcgttg agcgggtact
     2281 ggtcgtcgcc gcggctgtac gtgtgtgcgc caccgggaat aacatggtgg agccgtttcg
     2341 aataatcgtt cttgctcatt ggagaatgcc ctcttcgcgg gcttttagta aacgttcgat
     2401 gatgaggaaa tcgacgatat cgtcg
//
LOCUS       DASTSF010000347         4430 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1316476,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000347 DASTSF010000000
VERSION     DASTSF010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4430
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1316476"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /gene="rpsN"
                     /locus_tag="VFU15_09800"
     CDS             <1..219
                     /gene="rpsN"
                     /locus_tag="VFU15_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006743603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEU4718118.1"
                     /translation="ARYAEKRAKLKKEGKWTELSLLPRNSSPVRMRNRCKLTGRPRGY
                     NRQFGLCRNMLRELALNGKIPGVTKASW"
     gene            293..691
                     /gene="rpsH"
                     /locus_tag="VFU15_09805"
     CDS             293..691
                     /gene="rpsH"
                     /locus_tag="VFU15_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015813802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEU4718119.1"
                     /translation="MTDTISDYLTRVRNAIRANHRVVEVPSSRMKKEITKILFEKGYI
                     LNYKFDEAGNAQGVIKIALKYHPVTKQPAIRGLVRISKPGLRNYAGVTKMPRVLNGLG
                     IAIISTSKGIMTDKEAKKENVGGEVLCFVY"
     gene            712..1266
                     /gene="rplF"
                     /locus_tag="VFU15_09810"
     CDS             712..1266
                     /gene="rplF"
                     /locus_tag="VFU15_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003008516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEU4718120.1"
                     /translation="MSRIGKLPITLPKGVEISVSDKNVVAVKGPKGELKRTIDPAIKI
                     KVDGGVVSLERPTEQKRHKALHGLYRALISNMVKGVSAGYKTEQELVGVGYRAQAKGQ
                     QLELTLGYSHNVVFELPKEIKVTTTAEKGQNPKIIMECADNELLGLVAAKIRSLRKPE
                     PYKGKGIKFSNEILRRKAGKSAGK"
     gene            1313..1669
                     /gene="rplR"
                     /locus_tag="VFU15_09815"
     CDS             1313..1669
                     /gene="rplR"
                     /locus_tag="VFU15_09815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013996572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEU4718121.1"
                     /translation="MAITKSERRVRIKMRIRKRLSGTAGTPRLCVFRSNKDIYAQVID
                     DVKGTTLVSASSREKELAGKKVNKTEQAKKVGEAIARKAKDAGVSTVVFDRGGYLYHG
                     RVKALAEAAREGGLKF"
     gene            1702..2220
                     /gene="rpsE"
                     /locus_tag="VFU15_09820"
     CDS             1702..2220
                     /gene="rpsE"
                     /locus_tag="VFU15_09820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015547063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEU4718122.1"
                     /translation="MANVNVRRVKSSDIELKDRLVAVQRVTKVTKGGRTFSFTAIVVV
                     GNEKGVVGYGLGKAKEVTDAITKAIEDAKKNLMKVCIVNGTVPHPQVGKYGGSLVFLK
                     PASHGTGVIAGGAMRAVLESVGVTDVLAKSKGSSNPHNVVKATMDALMQMRDAFSVAQ
                     QRGITMEKVFNG"
     gene            2235..2414
                     /gene="rpmD"
                     /locus_tag="VFU15_09825"
     CDS             2235..2414
                     /gene="rpmD"
                     /locus_tag="VFU15_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEU4718123.1"
                     /translation="MSKIRITQIRSCIDYPERQKKTLQALGIRKMNVPVEKEVTPQVK
                     GMVERVKHLLKVESI"
     gene            2426..2875
                     /gene="rplO"
                     /locus_tag="VFU15_09830"
     CDS             2426..2875
                     /gene="rplO"
                     /locus_tag="VFU15_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002666677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEU4718124.1"
                     /translation="MNLSSLKPAKGSNKKEGKRLGRGQGSGKGGTSARGHKGAQSRSG
                     YSSKRGFEGGQMPLQRRLPKFGFRNISRVEYKGINLDSLQELIDKRKLSSIDPAILLE
                     NGLIGKNDLVKILGRGELKSKLDVKAHAFSASAKAAIEAKGGTATTL"
     gene            2877..4229
                     /gene="secY"
                     /locus_tag="VFU15_09835"
     CDS             2877..4229
                     /gene="secY"
                     /locus_tag="VFU15_09835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEU4718125.1"
                     /translation="MKKFIGTIKNIFAIEELRQRILFTLGFLLIYRLGSYVVIPGVDP
                     SALARAKQSDLGILQLINIFAGGAFNRASIFALGIMPYISASIILQLLTMALPYFQRL
                     QREGESGRKKINQFTRYLTVVITAMQAVGYLASQVVDKPGVVHDQSTFWWFQSVCVLV
                     TGTVFVMWLGEKITDKGIGNGISLLIMIGIVARLPFAISSEFGSRLESSGGGLIMFLV
                     ELAVLLAVILGCILLVQGTRRIPIHFAKRIVGNRQYGGQRNYIPLKVNAAGVMPIIFA
                     QAIVMIPIQLAGFAMKGSDGETSWFLQQFGNHYGIWYNLVTALLIIVFTYFYTAITVN
                     PKMMAEEMKRNEGYIPGIKPGNKTSEYIDQIMSRITLPGAIMLAIVAALPAIVIHFGV
                     SNEFAQFYGGTSILILVGVVLDTLQQIESYLLMRHYDGLMKSGRIKGRTSVGMATSAA
                     "
BASE COUNT         1218 a         1011 c         1153 g         1048 t
ORIGIN      
        1 gctcgttacg ccgaaaaacg cgcaaaactt aaaaaggaag gtaaatggac ggaattgtct
       61 cttcttccgc gtaactcgtc acctgttcgc atgcgtaacc gctgcaaact taccgggcgt
      121 ccgcgcggat ataacaggca attcggcctc tgccgtaata tgttgagaga actcgcattg
      181 aacggcaaaa ttccaggagt tacaaaagcc agctggtaag cacgacagcg gtccgcaaga
      241 aattgcgttt tgtataatta tcctgcgtgc gggataacat ataagacaaa aaatgacaga
      301 tacaatatca gactacctga cccgtgtcag gaacgccatc agggcgaatc atcgtgtcgt
      361 ggaagttccg tcttccagga tgaagaaaga gatcacgaag attctcttcg aaaagggata
      421 tattctcaat tataaattcg atgaggcagg aaatgcacag ggtgtgatca agatcgcgct
      481 caagtatcat cctgttacca agcagccggc tattcgcggg ctcgttcgta tttccaagcc
      541 cggcttgaga aattatgcag gcgtcaccaa gatgccgcgg gttctcaacg gtctcggaat
      601 tgcgatcatc tcgacttcca aaggcattat gaccgataag gaggcgaaaa aagaaaatgt
      661 gggcggagaa gtgctctgct ttgtgtatta atcaccggag gaggaacgaa catgtcacgt
      721 ataggaaaac ttccgatcac actgccgaaa ggagtggaga tttcagtgag cgacaaaaat
      781 gtcgtagctg taaaaggacc caaaggggaa ctgaagcgca caattgatcc ggcgatcaaa
      841 atcaaggttg acggaggcgt ggtgtctctc gagcgtccta ccgagcagaa aaggcataaa
      901 gcgcttcacg ggctttaccg tgccctgatc agcaatatgg taaaaggggt gtccgccggt
      961 tataaaacgg aacaggagct cgtaggtgtt ggatacaggg cccaggcaaa aggacaacag
     1021 ctggagctta ctttgggtta ttcgcacaac gtcgtgtttg agcttcccaa ggaaatcaag
     1081 gtcaccacta ccgccgaaaa ggggcaaaac cccaagatta tcatggagtg cgcggataat
     1141 gagctgctcg gacttgtagc ggccaaaata cgttcgctgc gcaaacctga gccttacaaa
     1201 ggaaaaggca tcaagttctc caatgaaatt cttcgccgta aagcaggtaa atctgctggt
     1261 aaataattaa aatatcatca cggcaaaaac cgtgagcaaa gacagataaa aaatggctat
     1321 caccaaatca gaacgcagag tccgtatcaa gatgcgcatc cggaagagac tctccggtac
     1381 cgcgggtact ccgcgtttgt gcgtattccg cagcaacaag gacatatacg cccaggttat
     1441 tgacgacgta aaagggacca cgcttgtttc agcttcctcg agggaaaaag agcttgcggg
     1501 taagaaggtc aataagaccg agcaggcgaa aaaagtaggg gaggccattg cccgcaaggc
     1561 aaaagatgcc ggcgtgagca cagttgtttt tgatcgtggc ggatacctct atcacggtcg
     1621 tgtcaaggcg cttgcggaag cggcccgaga aggaggtctt aagttctaac gatttaccca
     1681 gtcggggata aataaaaaaa gatggcaaac gtaaatgtac gtcgcgtaaa atcaagcgat
     1741 atcgaattaa aagaccggct tgttgctgtt cagcgggtaa ctaaagttac aaaaggcgga
     1801 cgcaccttca gctttaccgc aattgttgtg gtaggaaatg aaaagggcgt tgtcggttac
     1861 ggccttggaa aagcgaaaga agttacagac gcaattacca aagcaatcga ggacgcgaag
     1921 aagaacctga tgaaggtttg catcgtgaac ggaaccgttc ctcatccgca ggttggtaag
     1981 tacggcggat cgcttgtgtt cctcaagcct gcttcgcacg gtaccggtgt aattgccgga
     2041 ggtgctatgc gcgccgttct tgaaagcgtc ggtgtaacgg acgttcttgc caagagcaaa
     2101 gggtcttcaa acccgcacaa cgttgttaaa gcgacgatgg atgcgctcat gcagatgcgc
     2161 gatgctttct cggttgccca gcagcgcgga attaccatgg aaaaagtgtt taacggataa
     2221 taaaaaaata tcccatgtcg aaaatccgaa tcacgcagat cagaagttgc atcgactacc
     2281 cggagcgtca gaaaaaaacg ctgcaggctc tcggcattcg caaaatgaat gtgcctgtcg
     2341 aaaaggaagt gactccccag gtgaagggaa tggttgagcg tgtgaagcat cttctcaaag
     2401 tcgaatctat ttaactgaac aagcaatgaa cttaagcagt cttaaaccgg caaaaggttc
     2461 caataagaag gaaggaaaac gacttggccg cggccaggga tccggaaaag gtggaacttc
     2521 tgcacgtggt cataaaggag cgcagtcccg ttccggttat tccagcaagc gaggatttga
     2581 aggcggccag atgccgctcc agcgtcgtct gccgaagttc ggattcagga atatcagccg
     2641 cgtggaatac aaaggcatta accttgattc tctgcaggag ctgatcgaca aaagaaagct
     2701 ttcttccatt gatcctgcta tcctcctgga aaacgggctc atcgggaaaa atgatcttgt
     2761 aaaaattctg ggtcgcggag aattgaagtc aaaactggat gttaaagcgc atgctttctc
     2821 ggcttccgcg aaagctgcca tcgaagcaaa aggcggaaca gccacaacgc tctgatatga
     2881 agaagttcat aggaaccata aagaatattt tcgcaattga ggagttgcgc cagcggatac
     2941 ttttcacgct cgggtttctt ctcatttacc gccttggcag ctacgtagtt attcctggcg
     3001 tggatccttc tgcgcttgcc cgtgcgaagc agagtgacct gggaattctt cagctcatca
     3061 atattttcgc aggcggagct ttcaaccgtg cttccatctt tgcactgggt attatgccct
     3121 atatctctgc ttcgatcatt ctccagctgc tcacgatggc gcttccgtat tttcagcgtt
     3181 tgcagcgtga aggtgaaagc ggacgaaaaa agatcaacca gtttacccgt tacctgactg
     3241 tcgttattac ggccatgcag gctgtaggct acctggcatc tcaggttgtc gataagccgg
     3301 gtgttgttca tgatcagagc actttctggt ggttccagtc ggtatgcgtg cttgttacgg
     3361 gaactgtgtt tgttatgtgg ctgggtgaga aaatcaccga caaaggaatc ggtaacggta
     3421 tttcattgct catcatgata ggtatcgttg cgcgccttcc gttcgcgatc tcgtccgaat
     3481 ttggttcccg cctcgaatca tccggcggcg gtcttatcat gttccttgta gagcttgctg
     3541 tattgctcgc tgttattctc ggatgtattc ttctcgtcca gggaacacga cgcattccca
     3601 ttcattttgc aaaacgtatt gtcggaaaca ggcagtacgg cggacagcgc aattatattc
     3661 ccctcaaggt gaatgctgcc ggtgtaatgc cgatcatctt tgcacaggct atcgtaatga
     3721 tccctatcca gcttgctggc ttcgcaatga aaggatcgga cggagagacc agctggttcc
     3781 tgcagcagtt cggcaaccat tatgggatct ggtataacct tgtcacggcg cttctcatta
     3841 tcgtgtttac gtacttctat acggccatta ctgtaaatcc caaaatgatg gcagaggaaa
     3901 tgaaacgtaa cgagggttat attcccggga tcaagcctgg caacaaaaca tctgaataca
     3961 ttgaccagat catgtcacgg attacattgc cgggcgcaat tatgcttgcc atcgtggcgg
     4021 cgcttccggc gattgtgatt cacttcggcg tttccaatga attcgcgcaa ttctacggcg
     4081 gaacgagcat tctcattctt gtgggtgtgg tgcttgatac gctgcagcaa atagaaagct
     4141 atctcttaat gcgtcattac gacggcctga tgaaatccgg ccgtatcaaa ggtcgtacat
     4201 ctgttggcat ggcgacttct gcagcctgat gccggatatg atctattata agtcgcacga
     4261 agagatagag ttaataagac aaagttcttt acttgttggc aaagcccttg cagcagccgg
     4321 aagagaagtt aaaccgggtg ttaccacggc gtacattgac aaaattgccg aggattttat
     4381 tctcagccat ggaggcaaac cggctttcaa aggttatcgc ggttttccgg
//
LOCUS       DASTSF010000348        10894 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_911505,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000348 DASTSF010000000
VERSION     DASTSF010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 10894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10894
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_911505"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..552
                     /locus_tag="VFU15_09840"
     CDS             <1..552
                     /locus_tag="VFU15_09840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718126.1"
                     /translation="LILRCLAVQSDEDYRDVEELCRKVTRQTRTSRKPFAQVDFHFTD
                     QDGNAVSDYLFKLGYLQNEKKFPADFVACTHKNEVAPENFNAFLAMEDFESHRDYFFE
                     LDATSGSDLFSLQPDILTANAGGDSVSQLIVEDHTTQFEIVLYREPVRNLFVFHAGND
                     PDLHLRYDRLGVPKKGETGIKPE"
     gene            589..3054
                     /gene="priA"
                     /locus_tag="VFU15_09845"
     CDS             589..3054
                     /gene="priA"
                     /locus_tag="VFU15_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013752082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEU4718127.1"
                     /translation="MSSRVDYFADVILPLALPNAYTYRIPQSLNGEVKEGMRVVVQFG
                     KNKLYSAIVRKVHTVAPSYTAKYIDSVLDPVPVVTETQLKFWDWMADYYLCTRGEVML
                     AALPSGLRLSSETRVVLNPGWPGDRKLTDDEAFTVCEALDVRKTLSLEDISAILDRKT
                     VYPKIRQMLDEGIILVYEELQERFSPKFETFLALAPEYEEEEKLREVFDKLEKKAFKQ
                     LQVMMSFVHLSGRFEKKTVPVRQSDLVKASGADSSAIASLVKKGILLREELEVSRIIK
                     DISGEKEVVLSEHQRKAMEDISAPFREKDVALLHGVTSSGKTEVYMHLIRGLFASGKQ
                     VLYLLPEIALTTQLITRLQRHFGNKVLVYHSRFNENERVEVWREVLKGEPVVVIGARS
                     ALFLPFTALGFVIIDEEHDPSFKQHDPAPRYNARESGILLATLHGAKVLLGSATPSLE
                     SFYNAKHGRYGYASMTERYGGSTLPEITVVDIREASRKRLMKSHFSPLLLEKTEQALK
                     ANEQIIFFQNRRGFAPSIECQSCGHIPHCVRCDVSLTYHKNSNQLRCHYCGWTTNPPQ
                     QCEACGGTELKMKGFGTEKIEEELSLIFPGVKVARMDLDTTRGKHSLQKLVNDFETHA
                     IDILVGTQMVTKGLDFGNVALVGILQADQLMNFPDFRAYERSFQLMAQVSGRAGRREK
                     KGEVIIQTFNPQHQVIQCVISHDYETLYHAELAERMEFHYPPFYRLVRLAVKGKDATL
                     VDNGAFQLADNLRKHLGANILGPEYPPVSRVRDEFIKEILVKIERGTNPSGVKKIISA
                     EMIAMKAHSDFKKLRVVADVDPN"
     gene            complement(3215..4189)
                     /locus_tag="VFU15_09850"
     CDS             complement(3215..4189)
                     /locus_tag="VFU15_09850"
                     /inference="COORDINATES: protein motif:HMM:NF044334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718128.1"
                     /translation="MNKPFIVKIISAPLNFLVCALIAQLFILIQDGRGQGDIYAFLFW
                     TIPLAAALAFSGGTIVQLTEVKNFSVRLMLLFAVTVFVSSGWIFFVSIFLGGWINAFS
                     FPLLYPWIAGSFVQLGFLLWRLPEPATPVKRSRLLVKLLLFPAAFIIATIACIAVRSV
                     YLNLNGPEKELYLIPDNFNGDFRVVYGEKCGVLPESENGRRVMRIPADGVLIVQEEFR
                     AGTIDNVYYLVDAAGKRTKVNEIYDVSGRFSKPGASVMLSSTGSIAGKMPDESFSSES
                     PLAITYEDFTLFNKDTANADTEKNRDRLDSLTEAKVGECRATVKGNPK"
     gene            complement(4586..5347)
                     /locus_tag="VFU15_09855"
     CDS             complement(4586..5347)
                     /locus_tag="VFU15_09855"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEU4718129.1"
                     /translation="MSTEPEIGLVLSGGGARGFAHLGVLQALDELGIRIAAISGTSAG
                     AICGAFYFAGHRPQDTLRYIRSYKIYEWARILWRKPGILNMQKISEMFAKYLPDTFEG
                     LDRPLTVCATDILKGCPEYFSSGPLTPAICASAAIPLVFEPVSMNGSSYVDGGILDNF
                     PLGQLQKKFKTIIGVHVNPINHHLDHVHFHEVMDRSMHLALSPAVTDKKDQCSVFIEP
                     KGCENFGMFDLSGGEKIFAIGYEAAMSVKKELLKL"
     gene            complement(5365..6204)
                     /locus_tag="VFU15_09860"
     CDS             complement(5365..6204)
                     /locus_tag="VFU15_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015267126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU4718130.1"
                     /translation="MKLHTIETGFFKLDGGAMHGVVPKSLWKKENPPDDNNLCTWAMR
                     CLLVEEGNRLVLIDNGIGDKQDAKFFSHYYLHGDASLEKSLNAAGYSMNDVTDMFLTH
                     LHFDHCGGSIRYNGDRSKLVTAFPNATYWSNKEHWEWATKPNAREKASFLKENILPIE
                     ESGQLKFIEKGEELFPGFGVLFTYGHTGAMMHPKISYKGKTIVYMADLMPSVAHIPLP
                     WIMAYDVRPLETLKEKETFLPQAAKEEYVLFFEHDAVNECCTVTETERGVRVNEKFRL
                     SAL"
     gene            complement(6217..6648)
                     /locus_tag="VFU15_09865"
     CDS             complement(6217..6648)
                     /locus_tag="VFU15_09865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718131.1"
                     /translation="MRALSVIGIVLSMAGIIAAIYVMSASVCECNTNDNLFFDMKDQA
                     TPAAIRGGTIMLMFFVFFLVFSIIGTAMSFSPKKEAQPLPAYPQAPANTYPPFHPNQY
                     TVSNQWPQQQETQQAPPQQQNTWPQQEQNYDRWKPPGNSGS"
     gene            6921..8018
                     /gene="lpxK"
                     /locus_tag="VFU15_09870"
     CDS             6921..8018
                     /gene="lpxK"
                     /locus_tag="VFU15_09870"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HEU4718132.1"
                     /translation="MNLLRILAWPLSLLYGLIVTLRNKLFDIGILPSTNFGDVALIGV
                     GNLSAGGTGKTPHVEYLVRLLSSRYAVATLSRGYGRETSGFVLAESDATTSQIGDEPK
                     QFRHRFDPSVPVAVDSGRVHGVKKLLNQFPKLQVIILDDVFQHRSIKPGLQILLTDYS
                     RLFYKDQMLPTGYLREPRSGARRADVIVVTKTPQMFSPLERKRIMKEIRPEPYQKIYF
                     STIVYGDFIPFGNRKDLVTMEKEFCFSLNFSVVLLTGIANSHSLEYFLKDKVKELVPF
                     RFRDHHEFSPADLLRLKEIYEDVKNDNKIVITTEKDAMRLMKPGLEEYLGDLPVYYVP
                     IEIAFQDQDAQNFNQQILDYVRSHPVNSKLH"
     gene            7981..8796
                     /locus_tag="VFU15_09875"
     CDS             7981..8796
                     /locus_tag="VFU15_09875"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005845051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEU4718133.1"
                     /translation="MFDRTQSTANYIKEKTNDFSPETGIILGTGLGGLVHEIQAEYIL
                     PYETIPDFPVSTVEGHSGKLIFGKLSGKNVIAMQGRFHFYEGYSMQQVVFPVRVMKLL
                     GIKRLFVSNASGGVNPDFSIGDLMIITDHINLFPSNPLIGANDKRFGVRFPDMSEPYD
                     HGMIALAKKIAGELGHNVQEGIYVGMSGPTFETPAEYKYVRNIGGDTVGMSTVPEVIA
                     ARHMLVPVFAMSVITDLGVPGKIVEVTHEDVQKVAEIAEKKMTQIMKEMVGRM"
     gene            9502..>10894
                     /locus_tag="VFU15_09880"
     CDS             9502..>10894
                     /locus_tag="VFU15_09880"
                     /inference="COORDINATES: protein motif:HMM:TIGR04312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor B family protein"
                     /protein_id="HEU4718134.1"
                     /translation="MKRLLFLLAFAPVIAHSQTYDAQNVSMLGQWNNPSQPAEPTYGI
                     KYQGVWGWKDTTNNHEYGIIGSSTGVNFIDVTNPASPVMCDYVPSRHGNLIWHEIKTY
                     RNYCYVVSDDVSPNSFQIIDMSYLPDSVHVLYDDTTVFERCHTIFVDGDKLWCGSVTR
                     RNNNYYSMAAYDLSADPVHPQLIRVLHQDYGSPATVHDMFVRNDTVYASGGYDGLFIY
                     VLDDTLHHFHEIAALQNYPEQGYNHSSFLSQDGRTLIFTDEVPAGLGMKSLDVSDMSN
                     ITMNQVFRSNQGDTPHNPYIIGNNVLVSANYCDGIQVFNIGNPSNVVRTGYFDTDTLI
                     NYPNYTQAYHGAWGAYTDLPSGHILGADMQNGLYILDISQAILASVPENVSTVMLNAY
                     PNPFNTNFFINLGLDKAQDVTYTIFDNEGRMIREEKQSMPAGNSILEVDGEGLVPGMY
                     SVRVTGETVNGTSR"
BASE COUNT         2493 a         2821 c         2954 g         2626 t
ORIGIN      
        1 cttatcctgc ggtgtcttgc cgtgcaaagc gacgaagatt accgtgatgt ggaagaactt
       61 tgccggaaag tgacgaggca gacgcgcacc agccggaaac cctttgcgca ggtggatttt
      121 cattttacgg accaggatgg gaatgctgtc agcgattatc ttttcaagct gggttatttg
      181 cagaatgaga aaaaattccc ggcggatttt gtcgcctgca cgcacaaaaa tgaagttgcg
      241 ccggaaaatt tcaatgcgtt ccttgcgatg gaggatttcg aatcccaccg cgattatttt
      301 ttcgagctcg acgccacttc cggttccgac cttttttcat tgcagcccga tatcctcact
      361 gcaaatgcag gcggggattc cgtttcacag ctcatcgttg aagaccatac cacgcaattc
      421 gagatcgtgc tttaccggga gccggtgcgt aatctctttg ttttccatgc aggcaatgat
      481 ccggatcttc acctgcgtta cgaccggctg ggtgttccca aaaaaggaga aacaggaata
      541 aaaccggaat agcacatttt ccaccggcgc agcgtttaaa tttgtttcat gtcttcccgc
      601 gttgattatt tcgccgatgt cattttgccg ctggccttgc cgaatgcgta cacgtaccgc
      661 attccgcaaa gcctgaacgg ggaggtgaag gaaggcatgc gcgttgtcgt ccagttcggg
      721 aagaataaat tgtattccgc gatcgtccgg aaagttcata cagtggcgcc gtcctacacc
      781 gcgaagtaca tcgattccgt tctcgatcct gtaccggttg ttacggaaac gcagctgaaa
      841 ttctgggact ggatggcgga ctattacctc tgcacgcggg gagaagtgat gctggccgcg
      901 cttccttccg ggctgcgcct gtcgagtgaa acgcgcgtgg tgctgaaccc gggctggccg
      961 ggcgaccgca agctgaccga cgacgaagcg ttcaccgtgt gcgaagcgct cgacgtgcgt
     1021 aaaacacttt cgctggaaga catcagcgcg atcctcgacc ggaaaacggt ttacccgaag
     1081 atccgccaga tgctcgacga aggaattatt ctcgtttacg aggaactgca ggaacggttt
     1141 tcaccgaaat tcgaaacgtt ccttgccctg gcgccggaat acgaagagga agaaaagctc
     1201 cgcgaagtgt tcgataagct cgagaagaaa gcgttcaaac agctgcaggt gatgatgagc
     1261 ttcgtgcatt tgtcggggcg ctttgaaaaa aagacggtcc cggtgcggca gtcggatctc
     1321 gtgaaagctt cgggtgcgga ttcttcagcg atcgcatcgc tggtgaaaaa aggaattctc
     1381 ctgcgcgaag agctggaagt gtcgcgcatc atcaaagata tttcaggtga aaaagaagtt
     1441 gtgctgagcg aacaccagcg gaaagcgatg gaggatattt ctgccccgtt ccgcgaaaaa
     1501 gacgtggccc tgctgcacgg cgttacttct tccggtaaaa cggaagtgta catgcacctc
     1561 atccgcgggc tgttcgcatc ggggaagcag gtgttgtacc tgttgccgga aattgcgctg
     1621 acgacacagc tcatcacgcg cctgcagagg catttcggga acaaggtgct cgtgtatcat
     1681 tcgcgcttca atgaaaatga gcgcgtggaa gtgtggcgcg aagtgctgaa aggcgaacct
     1741 gttgttgtca tcggcgcacg atccgctctt ttccttccgt tcactgcgct cgggttcgtc
     1801 atcatcgatg aagagcatga tccttccttc aagcagcacg atcctgcgcc gcgctacaat
     1861 gcgcgggaaa gcgggatact gctcgccacg ctgcacggtg cgaaagttct tctcggttcc
     1921 gccacgcctt cgctcgaatc gttttacaat gcgaagcacg gacgatacgg ctacgcgtcg
     1981 atgaccgaac gttacggcgg atcgacatta ccggagatca cggtggtgga catcagggaa
     2041 gcttcgcgga aacgactgat gaaatcgcat ttttcaccgc tgctgctgga aaaaacggag
     2101 caggcgctga aagcgaacga gcagatcatc tttttccaga accgcagggg atttgcgccc
     2161 tccatcgaat gccagtcgtg cgggcacatt ccgcattgcg tgcgctgtga cgtcagcctc
     2221 acgtaccaca aaaattcgaa ccagcttcgc tgtcattatt gtggctggac gacaaatccg
     2281 ccgcagcagt gcgaggcctg cggcggaaca gagttgaaga tgaaaggctt cggcacggaa
     2341 aaaatagaag aggagctgtc gctgatcttt ccgggcgtga aagtggcgcg catggacctc
     2401 gacacaacgc ggggcaagca cagcctgcag aagctcgtga atgatttcga aacacacgcg
     2461 atcgatattc ttgtcggaac gcagatggtg acgaagggac tggatttcgg caatgtcgcg
     2521 ctggtgggaa ttttacaggc cgaccagctg atgaatttcc ccgatttccg cgcgtacgaa
     2581 cggagcttcc agctcatggc gcaggtttcg ggtcgcgcgg gacgacgtga aaaaaaagga
     2641 gaagtgatca tccagacatt caacccgcag caccaggtga tccaatgcgt gatcagccac
     2701 gattacgaaa cgctttacca cgccgagctg gcggaacgta tggaattcca ttacccgcca
     2761 ttttaccgcc tcgtgcgtct cgcggtaaaa ggaaaagatg caacgctcgt ggacaacggc
     2821 gctttccagt tggcggacaa tctccgcaag catctcggag cgaatattct cggcccggaa
     2881 tacccgccgg tttcccgtgt gcgcgatgaa ttcatcaagg aaattctcgt gaagatcgaa
     2941 cgcggaacga acccttccgg tgtgaagaaa atcatctcag cggagatgat cgcgatgaag
     3001 gcgcacagcg atttcaaaaa gctgcgcgtg gtggcggatg tggacccgaa ctgattcact
     3061 ccgccggctt ttcctgcaat tctcttttcc cgttttcatt gccggccgga gaacgcacgg
     3121 cggatcgttt caaagtaaat gcggacaggt tttttcatcg cgccgggaag aaacaagata
     3181 cttcataagc cggtgcaggt gaaaaatgct gttgtcattt gggattccct ttcaccgtgg
     3241 cgcggcattc gccgactttc gcttcagtga gcgagtcgag acggtcccgg tttttttccg
     3301 tgtccgcatt cgcggtgtct ttgttgaaga gtgtgaaatc ttcatacgtg atcgcgagcg
     3361 gtgattcgct ggagaagctt tcgtcgggca tttttcccgc aatgcttccc gtgctgctca
     3421 gcatgaccga cgctcccggt tttgaaaaac gcccgctgac gtcgtagatc tcattgacct
     3481 tcgttctttt tcccgccgca tccacgaggt aataaacatt gtcgatggtt cccgcacgga
     3541 attcttcctg cacgatgagg acgccgtccg ccggaatgcg catcactctc cgcccgttct
     3601 cggattccgg cagcacgccg catttttctc cgtacacgac acgaaagtct ccgttgaaat
     3661 tatcgggaat gaggtacaat tctttttcag gtccgttcag gttgaggtac accgaacgaa
     3721 ccgcgatgca ggcaatggtc gcgataatga atgcggcagg gaagagcagc agtttcacca
     3781 ggagacggct tcttttcacc ggcgtcgcag gttcgggaag gcgccacaaa agaaaaccga
     3841 gctggacaaa acttcctgcg atccacgggt aaagcagcgg gaagctgaat gcgttaatcc
     3901 atccgccgag aaaaatggaa acgaaaaaga tccatccgga agaaacaaaa accgtgacgg
     3961 caaagaggag catgagccgc acggaaaaat ttttcacctc tgtcagctgc acgattgttc
     4021 ctccgctgaa cgccagcgcc gcggcaagcg ggatcgtcca gaaaaggaaa gcgtaaatgt
     4081 cgccctgccc gcgcccgtcc tgtatgagaa taaacagttg ggcgatcaat gcgcagacga
     4141 gaaaattcag gggcgcgctg atgatcttta caatgaaagg cttgttcatg ggaaattttt
     4201 agcgccccga aagaaaggca tccccaaaag taggatagtc gcgcggaaag tcaagaggtg
     4261 tggccgcaca cgttcgaaat gaagctgccg ctttgtgtct tcccggtaaa agaaataagt
     4321 cacgaagatg tgatgatgac ggagcgcacg ggttgtgcga tgaatttcct gtgttcctgc
     4381 tttcgctatt gcacggcaaa gcgctcaatc agggctaagg aatgatcagc aggcaaacag
     4441 gaaccattga attctgaatt catgcattgc ccgcagatgc cagtcgcccc tgtcaggtcg
     4501 agcgcaagtc gagacctggc agggagagaa acataccggg cggcaaaaaa atcccgcaac
     4561 aacaaaaaac acgcaaaact ttcccttaca gcttcagcag ctcctttttc acgctcatcg
     4621 ccgcttcgta tccgatcgcg aaaatttttt ctccgccgga aagatcgaac atgccgaaat
     4681 tctcgcagcc tttcggttcg atgaaaaccg agcactgatc ttttttgtcc gtcacggccg
     4741 ggctcagcgc aagatgcatg ctccggtcca tcacctcgtg gaaatgcaca tgatcaaggt
     4801 gatgattgat gggattgaca tgaacgccga tgatcgtttt gaattttttc tgcagctgcc
     4861 cgagaggaaa attatcgagg atgccgccgt cgacgtagct ggaaccgttc attgaaaccg
     4921 gttcgaacac gagcgggatc gcagccgatg cacatatggc gggggtcagc ggacccgagc
     4981 tgaaatattc cgggcatcct ttgagaatgt cggtggcgca aacggtgagc ggccggtcga
     5041 gtccttcgaa cgtatcgggc aggtatttcg cgaacatttc cgagatcttc tgcatgttca
     5101 ggatgcccgg ttttcgccag agaatgcgcg cccactcgta aattttatag gagcggatgt
     5161 agcgcagcgt gtcctgcggg cgatgcccgg caaaatagaa tgcgccgcag atcgcgcctg
     5221 cgcttgttcc ggagatagca gcgatgcgga tgccgagctc gtccagcgcc tgcaatacgc
     5281 cgagatgcgc gaagccgcgc gcgccgcctc cgctcaatac caggccgatc tcaggttcag
     5341 tgctcaatgt ccgggattca atgttcagag tgcggacagg cggaactttt cattcacacg
     5401 cacgccgcgt tcggtttcgg tcacggtgca gcattcgttc acggcgtcat gctcgaagaa
     5461 gagaacgtat tcttctttcg ccgcctgcgg aaggaacgtt tctttttctt tcagcgtttc
     5521 gagcggacga acatcgtagg ccatgatcca gggaagagga atatgcgcga cggacggcat
     5581 gaggtcggcc atgtacacga tcgtttttcc cttgtacgaa attttcgggt gcatcatcgc
     5641 gcctgtgtga ccgtacgtga atagcacgcc gaagccggga aaaagctcct ctcctttttc
     5701 aatgaatttc agctgcccgc tttcttcgat gggaaggata ttttctttca ggaagctggc
     5761 tttttcgcgt gcattcggtt tcgtcgccca ttcccagtgc tctttgttgc tccagtaagt
     5821 cgcgttcgga aaagcggtga cgagctttga ccggtcgccg ttgtagcgaa tgcttccgcc
     5881 gcaatgatcg aaatgcaggt gcgtaaggaa catgtccgtc acgtcattca tgctgtatcc
     5941 cgcggcgttc agcgattttt ccagcgacgc gtcgccgtgc aggtaataat ggctgaagaa
     6001 tttcgcatcc tgcttgtcgc cgatgccgtt atcgatgagc acgaggcggt tgccttcttc
     6061 cacgagcagg cagcgcatgg cccaggtgca gaggttattg tcgtcgggag gattttcttt
     6121 tttccagagg cttttcggaa cgacaccgtg catggcgcct ccgtcgagct tgaaaaatcc
     6181 ggtttcaatg gtgtgaagtt tcataggcgg ttcagatcag ctgccggaat ttcccggcgg
     6241 tttccatcgg tcgtaattct gttcctgctg cggccaggtg ttctgctgct gcggaggtgc
     6301 ctgctgcgtt tcctgctgct gcggccactg gttgctgaca gtgtactggt tcggatggaa
     6361 aggaggatac gtgttcgccg gtgcctgcgg gtaggcggga agcggctgcg cttctttttt
     6421 cggcgaaaag ctcatggctg ttccgatgat ggaaaacacg aggaagaaaa cgaaaaacat
     6481 gagcatgatg gtgccgccac gtatggccgc cggcgtcgcc tggtctttca tgtcgaagaa
     6541 caggttgtcg ttcgtgttgc attcgcagac ggaagccgac atgacgtaga tggcagcgat
     6601 gatacccgcc atgctgagaa caataccgat aacagaaagt gcgcgcatga ttttttgcag
     6661 agtttaaagg tacaggttgc gcgcgtaatc tgccagcagg gaacggcacg gttttattgc
     6721 cgcagacgca aagacacgaa aagcaggttt cattcccggg gaaggcggca accgttaacc
     6781 ggcagaaaaa cgcgaagttg cggatgggaa tacatcttcc gtaaggtaaa acaaggttgc
     6841 tttccaccgg gaattcagta aacagaatgg tcacataaaa tctgccgttc gccttgctga
     6901 tttatgtatt ttcgctgcca atgaacctgc tccgcatcct cgcatggccg ttatcgctcc
     6961 tgtacgggct gatcgttacg ttgcggaaca agcttttcga tatcggcatc ctgccttcga
     7021 cgaatttcgg tgatgtggcg ctgatcggtg tgggaaatct ttccgccggg ggaacgggca
     7081 aaacaccgca cgtcgaatac ctcgtgcgcc tgctgtcgtc gcggtacgcg gtggccacgc
     7141 tgagccgcgg ttacggaagg gaaacatccg gattcgttct ggcggaaagc gatgcaacca
     7201 cttcgcagat cggcgatgag cccaagcaat tccgtcatcg tttcgatcct tccgttcccg
     7261 ttgccgtcga ttcgggccgc gtgcacggcg tgaaaaagct gctgaaccag ttccccaagc
     7321 tgcaggtcat cattctcgac gacgttttcc agcaccgcag catcaagccc gggctgcaga
     7381 tattgctcac cgattacagc cgcctttttt acaaggacca gatgctgccc acgggttatc
     7441 tccgtgaacc gcgttccggc gcaaggcgcg ccgacgttat cgtggtgacg aaaacgccgc
     7501 agatgttttc gccgctcgag cgcaagcgca tcatgaaaga aatccgcccg gagccgtacc
     7561 agaaaatcta tttctccacg atcgtttacg gcgatttcat tcctttcggc aaccggaaag
     7621 acctggtgac gatggaaaaa gaattctgct tctcgctgaa tttttccgtc gtgctgctca
     7681 ccggcatcgc caattcgcat tcgcttgaat atttcctgaa agacaaagtg aaggagctgg
     7741 tgcctttccg tttccgggat caccatgaat tttctcccgc cgatcttttg cgcctgaaag
     7801 aaatttacga ggatgtgaag aacgacaaca agatcgtcat caccaccgaa aaggacgcca
     7861 tgcgcctgat gaaacccggc ctggaagaat acctcggcga tctgcccgtt tattacgtgc
     7921 cgatcgagat cgctttccag gaccaggacg cccagaattt caaccagcaa atcctcgatt
     7981 atgttcgatc gcacccagtc aacagcaaat tacattaagg aaaaaacgaa tgatttttct
     8041 cccgaaacgg ggatcattct cggtaccggt ctcgggggac ttgtgcacga gatccaggcg
     8101 gaatacattc ttccctatga aacgatcccc gatttcccgg tgtctaccgt agaaggacat
     8161 tcgggcaagc tgatctttgg caagctttcc gggaagaacg tgatagccat gcagggacgt
     8221 tttcattttt acgaaggcta ctcgatgcag caggtggtgt tcccggttcg cgtgatgaag
     8281 ctgctcggga tcaaacgcct ttttgtttcg aacgcaagcg gcggcgtgaa cccggatttc
     8341 agtatcggcg atctcatgat catcaccgat catatcaatc tttttccttc gaacccgctg
     8401 atcggtgcga acgacaaacg tttcggcgtg cgttttcccg atatgagcga gccgtatgat
     8461 cacggcatga tcgcgctggc gaaaaagatc gcaggcgagc tcggtcataa cgtgcaggaa
     8521 ggaatttacg tcggcatgag cggcccgacc ttcgaaacgc ctgccgaata caagtacgtg
     8581 cgcaacatcg gcggtgatac cgttggcatg agcactgttc cggaagtgat cgctgcgcgt
     8641 cacatgctcg ttcctgtttt cgcgatgtcg gtgatcacgg atctcggcgt ccccggcaag
     8701 atcgtggaag tgacgcatga agatgtgcag aaagtggctg agatcgcaga gaaaaagatg
     8761 acgcagataa tgaaagagat ggtggggagg atgtgagtga tcattaaaat gctttctggt
     8821 ttcggtttca gtgtctgaat ggtcttcgtt ttctttcgtg cacttcgtga aaacctttgc
     8881 gtacttcgtg gttgaaacgg tttcaagttt cgggtttgtt ttcctttgtg cgctttgtga
     8941 aatactttgt gttcttcgtg gttgaaacgg tttcaagttt cgggtttgtt ttcctttgtg
     9001 cactttgtga aaacctttgt gtacttcgtg gttaaaacgg tttcaagttt caagtttcgg
     9061 gtttgttttc ctttgtgcgc tttgtgaaat actttgtgtt cttcgtggtt gaaacggttt
     9121 caagtttcaa gtttcgggtt gtttttcttt gtgcactttg taaaatgctt tgtgttcttc
     9181 gtagttaaaa cggtttcaag tttcgggttt gttttccttt gtgtactttg tgaaatgctt
     9241 tgtgttcttc gtagttaaaa cggtttcaag tttcgggttt gttttccttt gtgcactttg
     9301 tgaaaacctt tgtgtacttc gtggttaaaa cggtttcaag tttcgggttt gttttccttt
     9361 gtgcactttg tgaaaacctt tgtgtacttc gtggttgaaa cgattcccgt ttgttgttcc
     9421 aactttccga cttttcagct tgccaacttt gaacctggaa cgcccggaat tcgtatcttt
     9481 aatacagatc aatccttccc gatgaaacga cttttattcc tcctcgcgtt cgcacccgtg
     9541 atcgcgcatt cgcaaacgta cgacgcacag aacgtctcga tgctcggaca atggaacaac
     9601 ccttcccagc cggcggaacc gacgtacggt atcaagtacc agggcgtttg gggatggaag
     9661 gacacgacca acaaccacga atacggcatc atcggctcgt cgacgggcgt caacttcatc
     9721 gatgtcacga acccggcttc cccggtgatg tgcgattatg ttccttcgcg ccacggcaat
     9781 ctcatctggc acgagatcaa aacctaccgc aactattgct acgttgtcag cgatgacgta
     9841 tcgccgaaca gcttccagat catcgacatg tcttacctgc ccgattccgt gcacgtattg
     9901 tacgacgata cgactgtgtt cgagcgctgc cacacgatct tcgtggacgg cgacaagctc
     9961 tggtgcggca gcgttacgcg caggaacaat aattattatt ccatggccgc gtacgatctt
    10021 tccgctgatc ccgttcatcc gcagctcatc cgtgtgctgc accaggatta cggttccccg
    10081 gccacggtgc atgacatgtt cgtccgcaac gatactgttt atgcttcggg cggatacgac
    10141 gggcttttca tttacgtgct cgatgacacg ctgcatcatt tccacgagat cgccgcgctg
    10201 cagaattatc ccgaacaggg atacaaccac agcagctttc tttcacagga cggacgtacg
    10261 cttattttca cagatgaagt tcccgccggt ctcggcatga aatcgctcga tgtttcggac
    10321 atgagcaaca tcacgatgaa ccaggtgttc cgttccaacc agggcgacac gccgcacaac
    10381 ccgtatatca tcgggaacaa cgtacttgtt tccgccaatt actgcgacgg catacaggtc
    10441 ttcaacatcg ggaatccttc gaacgtggtg cgcacgggtt atttcgatac ggacacgctc
    10501 atcaattacc cgaattacac gcaggcgtac cacggcgcgt ggggtgcgta caccgatctt
    10561 cccagcggac acattctcgg cgctgatatg cagaacgggc tgtacatcct tgatatttcc
    10621 caggccattc tcgcttccgt tcccgaaaat gtgagcaccg tgatgctgaa tgcctacccg
    10681 aacccgttca acacgaactt tttcatcaac ctcggtctcg acaaagcgca ggatgtcacg
    10741 tacacgatct tcgacaatga aggccggatg atccgcgagg aaaaacaatc catgcccgcc
    10801 ggtaattcca ttctcgaagt ggacggcgag ggactcgtcc ccggcatgta ttccgtgcgt
    10861 gtgaccggcg aaacggtgaa cggaacgtcg cggc
//
LOCUS       DASTSF010000349        21453 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_241859,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000349 DASTSF010000000
VERSION     DASTSF010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 21453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21453
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241859"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(102..1748)
                     /locus_tag="VFU15_09885"
     CDS             complement(102..1748)
                     /locus_tag="VFU15_09885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718135.1"
                     /translation="MKKFLVIPFMLLCASLVAQTGGFSITAVGDVSPVLIDNVTKISD
                     PAVKKDTVLPAPSFDYTLQPKKFMTSVQIDTIKAAKLGNESVPKLYNTYARLGVGNYA
                     TITGEFYANSTRSKTSAWGFHARHFSAGKGPGDVQGDFSGFGQQQVDAFGKYFFRHHT
                     LTGGLDYDRHAVYNYGSDVAGNSFTKDFTKQTFNYWGANVDLLSHLPDSSDINHHGNL
                     RYYHLSDRYKVKEDNLLFSANGGTYLRGQMLNVDLGVDYNHVTGAQDTVNSTILHVTP
                     MIRSRGNKFDASIGIAVYGDLGAESFTYFYPQASFSYDIVNHIIVPYVTLGGYLERNS
                     MRTMTDINPFLMTTEAYALKNTQHKYELSGGLKGSISSEIVYDLRATRVELDNMPFFV
                     NTIESQDIFRNKFAAVYDNVDLVQAHAQLGWQHFEKVRLDLSGDYYHYTMTNELHPWH
                     TPTLRLGLLAEYNLQDKLIGRMDVCYLDGMYARMQNGSGYSVVNLKGLVDVNLGFEYR
                     YTKFFSVFLNLNNLANQRYERWYAYPTQRFNFLAGATYTF"
     gene            complement(1745..4891)
                     /locus_tag="VFU15_09890"
     CDS             complement(1745..4891)
                     /locus_tag="VFU15_09890"
                     /inference="COORDINATES: protein motif:HMM:NF024299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU4718136.1"
                     /translation="MRDIRSGLIFVFFFSLLLANASFAQQSQVYVNPDAKFKSALDLF
                     EKQKYAEAEEEFDQIVSAAKDKNDLLVIDAQYYAAICAYELFHKDAEERLKNFLSDHP
                     ESSRCQRVRFYLGRYNYRKKKYDDALDWFRLVEISDLQEDEIPEYYFKRGYSHFQLGH
                     LDSAKTNFYEIKDVDSKYAAPATYYYSHILYTEGSYETALQGFQKLNGDPTFGPVVPY
                     YIAQIYFLQGKYSDVISYAPPLLDSAKRAPEIAHLIGASYYRMNHFKEAIPFLEKYHA
                     GASSFSRDDAYELGYAYYRSDSAASAIVYFQDAIGDSSDALAQDAWYHLADCFLKTGN
                     RLGARNAFGKASGMKFDAVIREDALFKYAELSYELSFSPFNEAITALNEYLFEYPNTP
                     RHDEAYTLLTNVYLSTQHYKEALESIEKIKVLSPAMQGTYQMIAYNRGIELLNAKDYD
                     GAITHFDKTLTYPVSRELNSQSHYMKGETYYAKAMDKKDTASFTKAINEYKQFMVTPG
                     ATILPDYNTANYNIGYCYYQQKQWATSMIWFRKYIANKTTADANDKVFDAYLRLGDGY
                     FRMKDFLNSADFYAKAVAAPTTDDNMKDYAMFQEAMALGFQGKKQEKADMLKQLRTTY
                     PKSIYLADSRYQEARTLHDLRLYDQALESYRTLYNSNPGGPYGVSCLKNMGLIYRAKN
                     MPDSALVQYKKAVTAIHSSGPDFIDIMHEIKSIYVEKGQLEDWENYSASVGYVEAQAI
                     ADSTTYIVAKKFYSDGNCEGVQRECDKYVLKYPAGIYISEIYFMRAECAFKSNDLAKA
                     LDSYSAVLAKGQSGHTQRCLLQLAFIYYKQKNYASAAVMYHRIENESSDGNAQNDAKI
                     NLMRCYDFMGNADSAGIYANQVVTMKHLPNEVTGQAWYLKGKYDLSKNDKVQADKDFK
                     EVEKYVPNTDYAAEARYQQCWIKYSNGEFKTAEKALLKEINDYAGFSQWSGKGWLLLA
                     DDYLALKDTFQAKFVLQNYIDNGDVPELQQQAKDKLQAIETAQHKDVQKKEDDIIVPI
                     GDPGGGKPDDINSQGGGQ"
     gene            5188..5874
                     /locus_tag="VFU15_09895"
     CDS             5188..5874
                     /locus_tag="VFU15_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015809011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU4718137.1"
                     /translation="MSAEPIIELNNVSVFQKHNMVLTKVSATVCKGEFVYLVGKTGSG
                     KSSLMKTLYADVPLKEGSAKIAGFDLTSIRSSDVPFLRRKIGIVFQDFQLLYDRSVNE
                     NLFFVMKATGWKDKKAMLDRAQSVLDMVGLATKSHKMPHELSGGEQQRVSIARALLNN
                     PEVILADEPTGNLDPDTSSEIISLLMQISKNGTAVVVATHDMLVVDKYPARKLRCENG
                     VLIDSAAVSA"
     gene            complement(5871..6986)
                     /locus_tag="VFU15_09900"
     CDS             complement(5871..6986)
                     /locus_tag="VFU15_09900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEU4718138.1"
                     /translation="MSAKHLHIVSFDVPFPPSYGGVIDVFYKARALKELGVNIRLHCF
                     KYGRDESDALLSVADHVKYYPRRTETRLLFSAVPYIIASRTSEELLENISADNAPVLF
                     EGLHCCAYLGHPLLAGKKKIVRTHNIEHDYYRSLADVEKHLFRRMYFRREAAKLQKFE
                     KKLSIADAVLAISPADAMSLSMRYKNVHHVMAFHPYEEVAVGQTTADFALYHGNLEVG
                     ENNLAALFLVKEVFNDLGVPLVIAGNNPSDELVAAVKRSKNIRLEANIPTEKIDALIS
                     AARINVLPTFQATGIKLKLLAALFRGKHCIVNSPMIANTGLESLCTVADSAFAMKSAV
                     KRMYDAPFGISEVEKRKQVLGERFSNRKNAEKILALI"
     gene            complement(7034..8152)
                     /locus_tag="VFU15_09905"
     CDS             complement(7034..8152)
                     /locus_tag="VFU15_09905"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU4718139.1"
                     /translation="MKKAVVSVINDLVTDQRVHRTCVTLQGLGYDVLLVGRHKRDSLT
                     MPQRPYRTHRMNLHFEKGIWFYFFFNLRLYFFLRKQKADLFVANDLDTLWPNYRAARR
                     KKKPLVYDAHEIFTEVPELVNRKFKQRIWKRLERKLVPALKHMITVNDSIAGWYQDRY
                     GIRPEVVRNIPFYRPEAVHTLTRRQAHLPENKKIILLQGAGINIHRGAEEAVEAMKYV
                     DNAVLLIIGGGDVIPVLKEMTEKMHLTDKVIFTGKLPPDELRSWTRLADIGITLDKDT
                     NINYRFSLPNKIFDYIHAGIPVLASDLVEVRRVVEKYNVGKISPDHEPQHIAALLREM
                     LQSEEYPRWKENAAAAACELTGQREQEKQAAVFRQFLA"
     gene            8273..9280
                     /gene="murB"
                     /locus_tag="VFU15_09910"
     CDS             8273..9280
                     /gene="murB"
                     /locus_tag="VFU15_09910"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014681333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEU4718140.1"
                     /translation="MENDVSLRPYNTFGMNVSARRFSVVKATTELEELRKYSVKKQTP
                     LFILGGGSNILFTKDVDALVIKNELRGITVEKEDDDFVFVKAGSGEAWHSFVLDCIAH
                     NRAGVENLSLIPGSTGAGPMQNIGAYGVELKDVFHGLEAFDLRDGTMRKFTAEECRFG
                     YRESVFKHELRNRYFITSVTLRLNKKPVFNTSYGAIEKELEKMKVEKLSIAAISQAVI
                     NIRSSKLPDPKQTGNAGSFFKNPVVSAEKHAQLKKDFPEIVSYANGNDFKLAAGWLIE
                     QCGWKGKRAGDAGVHKDQALVLVNYGHATGKEIFDLSSEILASVKSKFGVELEREVNI
                     Y"
     gene            9449..10222
                     /locus_tag="VFU15_09915"
     CDS             9449..10222
                     /locus_tag="VFU15_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HEU4718141.1"
                     /translation="MKKQSNRQRLGWVPDLPDHRDHLYAAPPHIISRLPSSVDLRKQC
                     PPVYDQGDLGSCTANAIGAAIQFEQMKQKKKPFIPSRLFIYYNERAMEHTISSDSGAQ
                     IRDGIKSVNKLGVCPETLWPYSDDKTHFLKKPVAGCYKTALDNQVVSYQRVTQTLMQL
                     KGCLANGYPFVFGFTVYDNMYSAAVTKTGHLSLPGPNDKMEGGHAVMAVGYDDRNERF
                     IIRNSWGSKWAMKGYFTLPYSYITDSNLADDFWTIRMVE"
     gene            complement(10423..11679)
                     /locus_tag="VFU15_09920"
     CDS             complement(10423..11679)
                     /locus_tag="VFU15_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005678694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEU4718142.1"
                     /translation="MERIALSIPKVLKGEVKLPLSKSECNRLLVIRAITGTKVPVPQI
                     SDAEDTVSLRRILEQLETHTDGDTYDVGPAGTAMRFLSAYLSSIPGVFHLTGTARMKQ
                     RPIGILVKALQQLGADIEFPEKEGFPPLKIFGGPLTGGEIEMDGSVSSQFVSALLMIA
                     PTMHKGIVIRFKGPIVSRPYIDMTIRIMERFGVSPVWDGDTLSVSSQQYLVDDKEEQF
                     RLEPDWSAASYWYSMVALADEADIFLPGLHKNSMQADSVCALLFPFLGVTSTFEANGV
                     RLTKNGYRPNAFAFDFEDSPDIVQTVAVTAAALKIPTLMCGLNTLRVKETDRIAALKN
                     ELAKIGVKCEEPAAGLLDIRSFDGKTVSPLTISTYDDHRMAMAFAPLAIKHPGIVIEN
                     PGVVKKSYPGFWEEVKRVGGKMQPGT"
     gene            complement(11686..12780)
                     /gene="aroB"
                     /locus_tag="VFU15_09925"
     CDS             complement(11686..12780)
                     /gene="aroB"
                     /locus_tag="VFU15_09925"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013622536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEU4718143.1"
                     /translation="MIRQISTIASPRYPVHIGEYVPDSLNSFLKKNKFSSVFILVDEH
                     TLEHCYPVLVSRVKMLAEAEIIELESGEKNKTVDVCMNVWRALSELGADRKSLLINLG
                     GGVITDLGGFVASAYKRGITFINIPTTLLAQVDASVGGKTGVDLDGLKNEIGLFSEPA
                     ELFIYPEFLRTLPGREILSGFAEMLKHGLVADADYWNELKNVHPADSSSWEELIVRSV
                     RIKLSVVEADPHEAGLRRVLNFGHTVGHALETFFLERAESTLLHGEAVAAGMVCEAFL
                     SHTFASLSGEAMEEITATILAKYPPVIFDAMADHRLIELMRHDKKNEGASIVFTLIDE
                     IGKPVINKNVDAAGILRSLAYYRTSLNQSH"
     gene            12880..14046
                     /locus_tag="VFU15_09930"
     CDS             12880..14046
                     /locus_tag="VFU15_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015807345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HEU4718144.1"
                     /translation="MISFDNTEIAFSGKTDKDLRRSYWLFKMISSPAFVNFGKAMTNF
                     ALAIHLPIRWIIKPTIFRQFVGGEDIAECSRTVKELGKFRIGTILDYSVEGKETEADF
                     DRGAKEIIATIERAKNDVHIPFCVFKVTGLARFALLEKVTSGKTLTAEEEQEWKRVHG
                     RVENICGTAASFGKPIFIDAEESWIQQAIDDLADAMMQLFNKEKCIVYNTFQLYRKDR
                     LDFLRKSFEKAQQGNYFLGAKLVRGAYMEKERARASQMSYPSPIQETKADTDRDYDEA
                     VKFCIAHVDRVGLCAGTHNEKSSQQLAQLLDEKKITHDHPHVFFSQLLGMSDHISFNL
                     SHEGYNVAKYVPYGPVRDVLPYLIRRAQENTSVKGQTGRELNLIIREKERRKGR"
     gene            14263..15513
                     /locus_tag="VFU15_09935"
     CDS             14263..15513
                     /locus_tag="VFU15_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018476135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEU4718145.1"
                     /translation="MAESLIGSEIIKLAADINEKIKNGETVYNFTIGDFNPKLFPIPQ
                     ELKKFIIDAYNNDETNYPAANGVAELRSAVSNLIEKRQGLKYSPDEFLIAGGARPIIY
                     AIYRTIVENGDKVLFPVPSWNNNHYTHLCTGDYANQVFVETKPENNFMPTAEELRPHL
                     SKVSLVAVCSPLNPTGTTFSKKDLSEICDLILAENARRGPGEKPLYLLYDQIYWALTY
                     GDTVHYDPVSLRPEMRPYTIFVDGISKSLAATGVRVGWAMGPKAIIDRMKSILSHVGA
                     WAPKAEQVATAKFLSDLSAYDKFLGEIKQKISTRLTAFHKGFQDLKKKGYAVDSIAPQ
                     AAIYLTIRFSLHGKKTPDGKVLATTKDITGYILNEAKMAVVPFYAFGASPDSSWYRLS
                     VGTCRPEDIEAIMGSLEKALSKLS"
     gene            15534..16625
                     /locus_tag="VFU15_09940"
     CDS             15534..16625
                     /locus_tag="VFU15_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018476983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HEU4718146.1"
                     /translation="MKKNRFCVIMAGGVGTRFWPMSRSGHPKQFIDILGTGQTLLQST
                     FSRSLRICPASNIFVVTNENYFDLVREQLPKLPPENILCEPARKNTAPCVAYASWKIH
                     SIDPDAVMVVAPSDHLIMKEDTYVKAIKSCMRIAENEDQLLTIGIRPTRPDTGYGYIQ
                     FVNTEKDRKDKRIKKVKTFTEKPDLDMAKFFLQSGDFLWNSGIFVWSVKSVMTAFHRY
                     STDLFQIFEEGKGLWNTKKEKNFLKDAYSRCKNISIDYAILEKADNVLVRASIIGWSD
                     LGTWGSLYSHIRKDDHGNAVVGKQVMLYNSGNCVVHVPKDKLVVIDGLDDYIVVESDN
                     ILLICKKQDEQQIRTFVNDVKIAKGERFV"
     gene            16622..17065
                     /locus_tag="VFU15_09945"
     CDS             16622..17065
                     /locus_tag="VFU15_09945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718147.1"
                     /translation="MKKIILLPAIVFFFSCHSSPEKVSPDPVGTWKPHVMWDNNDITL
                     QVLPDSMIKFKAVKGFCPGIKTFVSVGKWTIVHDSILQMTQYTDGRRYTMKQLFPELV
                     LPNDSVMPDVIPLDMNARFIIAKDHLYDVELDGKRSVKRYYDRKK"
     gene            complement(17076..17669)
                     /locus_tag="VFU15_09950"
     CDS             complement(17076..17669)
                     /locus_tag="VFU15_09950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718148.1"
                     /translation="MSRLNYIFLFLFFPLFLAAQKDSVDVKKEIVINNKRFRVYNSWV
                     SGGVGLAYHSVDPRTQTMLGVNFNFHIKQYYFRLGTMFSGDVFGLWNNFQAHACWIPY
                     RTDQVKYHLAMMAGASYSTGYPYLYAGTFDNIHPYKETGLYLEVQYIRKLFFDVGGGG
                     ALFANINKQNTIIGVRADIYLSGAYKGYVKGKEPHHM"
     gene            complement(17785..19182)
                     /gene="fumC"
                     /locus_tag="VFU15_09955"
     CDS             complement(17785..19182)
                     /gene="fumC"
                     /locus_tag="VFU15_09955"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008240073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HEU4718149.1"
                     /translation="MNYRIEKDTMGEVKVPADKYWGAQTERSRNNFKIGPEASMPKEI
                     IYAFAYLKKAAAHANMDLGVLPKEKCDLISQVCDEILAHKLDDQFPLVIWQTGSGTQS
                     NMNANEVIAYRAHVISGGQLTDEKKVLNPNDDVNKSQSSNDTYPTAMHIAAYKLVSEV
                     TIPGMEKLRDALDKKAKEFSSIVKTGRTHFMDATPLTLGQEFSGYVQQITNSIRAVKN
                     ALEMVRELALGGTAVGTGLNTPKGYDVLVAKKIAEFTGFPFVTAPNKFEALAAHDAMV
                     ELSGALKRSAVALMKVANDIRMLSSGPRSGIGEIIIPDNEPGSSIMPGKVNPTQPEAL
                     TMVCAQVMGNDVAVSIGGATGHFELNVFKPLIAYNVLQSARLLGDACVSFTEKCAGGI
                     QPNLPVIRQHLENSLMLVTALNTHIGYYKAAEIAKKAHKENKTLRATAIELGYVTPEQ
                     FDQWVRPEDMVGSMK"
     gene            complement(19260..20291)
                     /locus_tag="VFU15_09960"
     CDS             complement(19260..20291)
                     /locus_tag="VFU15_09960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718150.1"
                     /translation="MRLFLLFLFPFFLGACHQPHATVATRPPAVPAETIATVIHAAEH
                     NLDWTDIAVAAATVNSPHSGSNPDYTKYRYEWRGKMYEGVEHEDIYLAEGDKFIVLIP
                     DTLRPYESKLLLENPVFGEQELITLRAGYVRGASEKCIVYDYEYNGRRYRKQLYKRDA
                     PFSINGGMTPKQLILVLVDPANPARSIAVPDEILSREDSVRYSLPHSLPQLNYGMWLS
                     RGVVTGTPDTLVDAVGAYCYVPDTTELHAMKNLVGEWQFPYVRSQRLAVQQQQERLLR
                     ENGVPLYYTHKKKIGLRMPDSSVMILDLAAYQNSCSIALFSGYAPPQVRDGYIDSRAY
                     VYKNYFGGR"
     gene            complement(20312..20671)
                     /gene="arsC"
                     /locus_tag="VFU15_09965"
     CDS             complement(20312..20671)
                     /gene="arsC"
                     /locus_tag="VFU15_09965"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020527538.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HEU4718151.1"
                     /translation="MGNSKITIWHKNTCAKSCEVMKVLKDEGVKPSVFEYLVTPPTEE
                     QLRDILKKLGIPAEELIRKKEPIYKEKFAGKKMTEEKWIKAMVKYPILIERPIIIKGN
                     KAIIGRPTEKVIPFVES"
     gene            complement(20750..>21453)
                     /gene="prfB"
                     /locus_tag="VFU15_09970"
     CDS             complement(20750..>21453)
                     /gene="prfB"
                     /locus_tag="VFU15_09970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008508731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEU4718152.1"
                     /translation="DWASMLMRMYIMWGEKNGFKVKEADLQQGETAGIKSCTLEIEGD
                     YAFGYLKGENGVHRLVRISPFDSNARRHTSFASVYVYPLIDDTIEIEVNPSECEWETF
                     RSGGAGGQNVNKVETAVRLYHKPTGIIIKNQESRSQMTNRENAMRLLKSQLYEIEMRK
                     RMETVQAIRGNMKKIEWGSQIRNYVLHPYKLVKDLRTDYETSDAQGVLDGDLNEFVKK
                     YLMEYGASWNMELKT"
BASE COUNT         4959 a         5725 c         5680 g         5089 t
ORIGIN      
        1 aatggcagaa gggccatttt tatcaacaaa aatgttgaaa aaacgaggcc ctggaagttg
       61 ctcctgaaaa gcttttttat accccttttt ttcttacccg ttcaaaaagt atacgtagcc
      121 cctgcgagga agttgaaacg ctgcgtggga taagcgtacc atctttcata acgctggtta
      181 gcgaggttgt tgagattgag gaagacggag aaaaatttcg tgtagcggta ttcgaagccg
      241 agattcacgt caacgagtcc tttcaggtta acgacagaat agccgcttcc gttctgcatg
      301 cgcgcataca tgccgtcgag gtagcaaaca tccatgcggc cgatgagctt gtcctgcagg
      361 ttatattctg cgagcaggcc gagacgaagc gtaggcgtat gccacggatg cagctcgttc
      421 gtcatcgtgt agtgataata atcgccggaa agatcgagac gcactttttc aaaatgctgc
      481 cagccgagct gcgcatgcgc ctggacgaga tcgacgttat cgtacacggc agcaaacttg
      541 ttccggaaga tatcctgcga ttcaattgta ttcacgaaga agggcatatt atcgagctct
      601 acgcgcgtgg cgcggaggtc gtagacgatc tcggagctga tgctcccttt gagtcccccg
      661 ctgagctcat acttgtgctg cgtgtttttc agcgcatacg cttctgtggt catcaggaac
      721 gggttgatat ccgtcatcgt gcgcatgctg ttgcgttcga ggtaaccgcc gagcgtaaca
      781 tagggcacga taatatgatt gacgatgtcg tagctgaaag aagcctgcgg atagaaataa
      841 gtgaacgatt ccgcaccgag atcgccgtaa accgcaatgc cgatcgacgc gtcgaattta
      901 tttccgcgcg aacggatcat cggtgtgacg tgaaggatcg tgctgttcac tgtatcctgc
      961 gctcccgtca catggttgta atcaacgccg agatccacgt tcagcatttg cccgcgcagg
     1021 taagttccgc cgtttgcgct gaagagcagg ttgtcttcct tcaccttgta ccggtccgac
     1081 aaatgataat agcgaagatt gccgtggtga ttgatatcgg atgaatccgg gagatgactg
     1141 agcaggtcga cgtttgcgcc ccagtaattg aacgtctgct ttgtaaaatc cttcgtgaag
     1201 ctgttacctg cgacgtcgct gccataattg taaacggcgt gacggtcgta atcgagcccg
     1261 cccgtcagcg tatgatggcg gaaaaaatat ttcccgaacg cgtccacctg ctgttgcccg
     1321 aaaccggaaa aatcgccctg cacatcgccg ggacctttgc ccgcggaaaa gtggcgtgcg
     1381 tggaagcccc atgcgctggt cttcgagcgc gtgctgttcg catagaattc accggttatc
     1441 gtcgcgtaat tgccgacacc gaggcgcgcg taggtgttgt acagcttcgg cacggattca
     1501 ttcccgagct tcgccgcttt gatcgtatcg atctgcacgg aggtcatgaa ttttttcggc
     1561 tgcagcgtgt aatcgaacga cggcgcgggc agcacggtgt cttttttcac tgcggggtcg
     1621 gagatcttcg tgacgttgtc gatcagcacc ggggaaacat cgccgacggc cgtgattgaa
     1681 aatccgccgg tctgcgcaac gagcgaagcg cagagcagca tgaatggtat gaccaggaat
     1741 ttcttcattg tccacctccc tgtgaattga tgtcgtcggg ttttccgcca ccgggatcgc
     1801 cgatcggaac gatgatgtcg tcttctttct tctgcacgtc cttgtgctgc gccgtttcga
     1861 tcgcctgcag cttatctttt gcctgctgct gcaattcggg aacatcgcca ttgtcgatgt
     1921 aattctgcag cacgaacttc gcctggaacg tatctttcag cgcgaggtaa tcgtcggcca
     1981 gcagcagcca gcccttcccg ctccactgtg aaaacccggc ataatcgttg atctctttca
     2041 gcaacgcttt ttcagcggtt ttgaattccc cgttgctgta tttgatccag cactgctggt
     2101 aacgcgcttc agcggcataa tccgtattcg gaacatattt ctccacctct ttgaaatctt
     2161 tgtccgcctg caccttgtca ttcttcgaca ggtcgtattt gcctttcagg taccaggcct
     2221 gtcccgttac ttcattcggc aggtgtttca tggtcaccac ctggttggca taaattccgg
     2281 cagaatccgc gttgcccatg aaatcatagc accgcatgag atttattttc gcatcgttct
     2341 gcgcattacc gtctgaagat tcgttttcga tgcggtgata catcacagcc gcggaagcgt
     2401 aatttttctg cttgtaataa ataaaggcaa gctggagaag gcagcgctgc gtatgacccg
     2461 attgtccttt tgcaagcacg gcgctgtagc tgtcgagcgc tttcgcaaga tcattggact
     2521 tgaacgcgca ttccgcacgc atgaaataaa tttccgagat gtaaatccca gcaggatatt
     2581 tcagcacgta cttgtcgcat tcccgctgaa cgccttcgca attcccgtcc gagtagaatt
     2641 ttttcgcgac gatatacgtc gtgctgtcgg cgatcgcctg cgcttcgaca taaccgacgg
     2701 aagcggaata attctcccag tcttccagct ggcctttttc gacgtagatg gattttatct
     2761 cgtgcatgat gtcgatgaaa tccgggccgg aagaatggat cgccgtcacc gcttttttgt
     2821 attgcacgag cgcgctgtcg ggcatatttt tcgcacggta aataagtccc atgttcttga
     2881 ggcaggaaac gccgtaagga ccgccgggat tggaattgta caaggtgcga taagactcca
     2941 gcgcctggtc gtacaaacgc aggtcgtgca gcgtgcgcgc ttcctggtaa cgcgagtccg
     3001 cgaggtagat cgatttcggg tatgtcgtac gcagctgttt gagcatgtcg gctttttcct
     3061 gcttctttcc ctggaagccg agcgccatcg cttcctggaa catggcgtaa tccttcatgt
     3121 tgtcgtccgt cgtcggtgca gcgaccgctt tcgcgtagaa atcagccgag ttgagaaaat
     3181 ccttcatccg gaaatagccg tcgccgagac gcaggtacgc atcgaaaact ttatcgttcg
     3241 catcggctgt tgttttgttc gcgatgtatt tccggaacca gatcatcgac gtggcccact
     3301 gtttctgctg gtagtagcaa tagccgatgt tgtaattcgc cgtgttgtaa tcgggaagga
     3361 tcgttgcgcc cggcgtcacc atgaactgct tgtattcatt gatcgctttc gtgaacgatg
     3421 ccgtatcctt tttatccatc gctttcgcgt aatacgtttc tcctttcatg taatgcgact
     3481 gcgaattcag ctcgcggctc acgggatagg tcagcgtttt atcgaaatgc gtaattgcgc
     3541 cgtcataatc ctttgcattc agcagctcga tgccgcggtt ataggcgatc atctggtacg
     3601 ttccctgcat ggcaggactg agcaccttta ttttttcgat ggattcaagc gcctccttgt
     3661 aatgctgcgt gctgagatac acattcgtca gcagcgtata cgcttcatcg tgccgcggcg
     3721 tattcgggta ttcgaagagg tattcattga gcgcggtgat ggcttcgttg aacgggctga
     3781 aagaaagttc gtagctgagc tcggcatatt tgaaaagcgc gtcttcgcgg atgaccgcat
     3841 cgaacttcat gccggacgct ttcccgaacg cattgcgcgc gcccagcctg ttgccggttt
     3901 tgagaaagca atccgcgagg tgataccacg catcctgcgc cagcgcatcg gaagaatcgc
     3961 cgatcgcatc ctggaaataa acgatcgcgc ttgccgcact gtcggatcgg taataggcat
     4021 agccgagctc atacgcgtcg tcgcgcgaga atgacgaagc gcctgcgtgg tatttttcga
     4081 ggaaagggat cgcctccttg aaatgattca tgcggtaata ggaagcgccg atgaggtgcg
     4141 cgatctccgg ggcgcgtttg gcggaatcga gcagcggcgg tgcgtatgaa atgacatcgc
     4201 tgtattttcc ctggaggaaa tagatctgcg caatgtaata cgggacgaca ggaccgaagg
     4261 tcggatcgcc gttcagcttc tggaacccct gcagcgcggt ttcgtagctt ccttccgtat
     4321 acaggatgtg cgaataataa tacgtcgcgg gcgcagcgta tttcgaatcg acatccttga
     4381 tctcgtagaa attcgttttt gccgaatcga gatgaccgag ctggaaatgc gaatacccgc
     4441 gtttgaaata atattccggg atctcgtcct cctgcaggtc gctgatctcg acgagccgga
     4501 accagtcgag cgcatcatcg tatttctttt tgcggtaatt gtaacggccg aggtaaaaac
     4561 ggacgcgctg gcagcgggaa gattccgggt gatcggaaag aaaattcttc aggcgctctt
     4621 ccgcgtcctt gtgaaaaagc tcatacgcac atatcgcggc gtaatactgc gcatcaatga
     4681 ccagcaggtc gtttttgtct tttgcagctg aaacgatctg gtcgaattct tcttccgctt
     4741 cggcgtattt ctgcttttca aaaagatcga gcgcgctttt gaacttcgca tcgggattaa
     4801 catacacctg cgactgctgc gcgaatgatg cattggcgag aaggagcgag aaaaagaaga
     4861 cgaagatgag acctgaccgg atgtcacgca ttactgcggg ttttagcgac taaaattagt
     4921 acgggtcatt ccataggtcg gtcaaacagg ttccaagtta tcaacgcgaa cgtgttgaaa
     4981 ttattgccgc gtttcttctt tcgactggta ttaaagccat tgccgcaaac ctgcttttcc
     5041 cgctctaacg tgattctaat tcagtgcgca ttccgcttat gaatttccaa taggaaaatg
     5101 ttttccggga aaaccattcc ggcattttca ttccgccgca tttttcctgc gttcatgaaa
     5161 tctcattacg tttgcaaaag aaagttaatg agcgcagaac caatcatcga actgaacaat
     5221 gtttctgttt tccagaagca caacatggtg ctgacgaaag tttcagccac cgtgtgcaaa
     5281 ggagaattcg tttacctcgt cgggaaaaca ggaagcggga aaagctcgct gatgaaaacg
     5341 ctgtacgcag acgttccgct gaaagaaggt tccgcgaaga tcgccgggtt cgatcttacg
     5401 tccatccgca gcagcgacgt cccttttctt cgccggaaga tcggcatcgt attccaggat
     5461 ttccagcttc tttacgaccg ttcggtgaat gaaaatcttt ttttcgtgat gaaggcgacg
     5521 ggctggaaag acaaaaaagc catgctcgac cgcgcgcagt cggtgctgga catggtcggg
     5581 ctcgccacca aatcgcacaa gatgccgcat gagctttccg gcggcgagca gcagcgcgtt
     5641 tccattgcgc gtgcattgct gaataatccc gaggtcattc tcgccgacga gcccaccggg
     5701 aacctcgatc ccgatacgtc gagcgagatc atttccctgc ttatgcagat cagcaaaaac
     5761 ggaacggccg tcgtcgttgc cacgcacgac atgctggtcg tggacaaata cccggcacgc
     5821 aagcttcgct gtgaaaatgg tgtgctgatc gattctgccg cagtctccgc ttagatcagc
     5881 gcgaggatct tttccgcatt tttcctgttc gaaaaacgtt cgccgagcac ctgctttctc
     5941 ttttccacct cgcttattcc gaacggcgca tcgtacattc tcttcaccgc cgatttcatc
     6001 gcaaatgcgg aatccgccac cgtacacagg gattccagcc cggtgttggc gatcataggg
     6061 gaattcacga tgcaatgctt cccgcggaaa agagccgcaa gcagcttcag ttttattccc
     6121 gtcgcctgga acgtaggcag gacatttatc cgcgccgcgg agataagcgc gtcgatcttt
     6181 tccgtcggga tgttcgcttc aagacggatg ttcttgctgc gctttacggc agcgacgagc
     6241 tcatccgacg ggttgtttcc cgcgatcacc agcggaacgc cgaggtcatt gaaaacttcc
     6301 ttcacgagga aaagcgcggc gaggttgttc tctcccactt ccaggttacc gtgatacagc
     6361 gcgaaatcag cggtcgtttg cccgacagca acttcttcat acgggtgaaa cgccatgacg
     6421 tgatgcacat tcttataccg catggaaagc gacatcgcat cggcaggact gatagcgagc
     6481 acggcatcgg cgatcgataa tttcttttcg aatttctgca gcttcgctgc ttcacggcgg
     6541 aaatacatcc ggcggaaaag gtgcttttcc acatcggcga gcgaacggta ataatcgtgc
     6601 tcgatgttgt gcgtgcgcac gatctttttc tttccggcaa gcagcggatg accgaggtat
     6661 gcgcaacagt gcagcccttc gaaaagaaca ggtgcattat ccgcggaaat attctccagg
     6721 agctcttcgg aggtccgtga agcaatgata tacggaacag cggagaacag caggcgcgtt
     6781 tcggtcctgc gggggtaata cttcacatga tccgcaacgg aaagcagcgc atcgctttca
     6841 tcgcgcccgt atttaaaaca atgcaggcgg atgttcacgc cgagctcttt cagcgcgcgg
     6901 gctttgtaga aaacgtcaat gacaccgccg tagctcgggg gaaaagggac gtcgaatgaa
     6961 acgatatgaa ggtgttttgc ggacaagaat tctggtctgc gaatttcgca tttcctttgg
     7021 aaccggcggt aagttacgcg agaaattgac gaaaaacggc ggcctgcttt tcctgctcgc
     7081 gctgtcctgt gagctcacac gctgctgccg cagcattctc tttccagcgc gggtattctt
     7141 cactctgcag catttcacgc aggagcgcgg cgatgtgctg cggttcgtga tcgggagaaa
     7201 ttttcccgac gttgtatttc tccacgacgc ggcggacttc cacgagatcg gatgccagca
     7261 ccgggatgcc ggcgtggatg taatcgaaga ttttgttcgg cagcgaaaaa cggtaattga
     7321 tattcgtgtc cttgtcgagt gtgatgccta tatccgcgag gcgcgtccag gaacgcagct
     7381 catcgggagg aagcttcccg gtgaagatca ctttgtcggt aaggtgcatt ttttccgtca
     7441 tctctttgag cacaggaatg acatcaccgc cgccgatgat gagcagaacg gcattgtcaa
     7501 cgtacttcat cgcctccacg gcttcctctg cgccgcggtg aatgttgatc ccggcgccct
     7561 gcaggagaat gatctttttg ttctcgggca gatgcgcctg cctgcgtgtc agcgtgtgta
     7621 cggcctccgg gcggtagaac gggatattgc gcaccacttc cgggcggatg ccatacctgt
     7681 cctggtacca ccccgcgatg gaatcattca ccgtgatcat gtgcttcagc gcgggcacga
     7741 gtttccgttc gagccgcttc catattctct gtttgaattt ccggttgacc agctcaggca
     7801 cttccgtaaa aatttcgtgc gcatcgtaaa caagcggttt ctttttccgg cgggctgcac
     7861 gatagttcgg ccagagcgtg tccaggtcgt tggcgacgaa caggtcggcc ttttgctttc
     7921 ggaggaaaaa ataaagccgg aggttgaaga aaaaatagaa ccagatccct ttttcaaaat
     7981 gaaggttcat gcggtgggta cggtaagggc gctgcggcat ggtaaggctg tcgcgcttgt
     8041 gccgccctac gagcagaaca tcgtaaccga gtccctgcag cgttacgcac gtgcggtgaa
     8101 cacgctggtc ggtgaccagg tcattgatga cagaaacgac cgcctttttc atccgccgcg
     8161 aagatacgac gggggaatga tgtaccattt acgatgaacc atttacgacg gacgatttgc
     8221 gatttgcggc tgtcttaatt cccggatcgt acctactttt acgcctgtga acatggagaa
     8281 cgacgtttcg ctccggccct acaatacatt cggcatgaat gtttccgcgc gccggttctc
     8341 ggtggtgaaa gcaaccacag agctggaaga gctgaggaaa tattccgtga aaaaacaaac
     8401 gccgcttttc attctcgggg gcggcagcaa tattcttttt acaaaagacg tcgatgcgct
     8461 ggtgatcaaa aacgaattgc gcggcatcac cgtcgaaaaa gaagatgacg atttcgtttt
     8521 cgtgaaagca ggttccggcg aagcatggca ttcgttcgtg ctcgactgca tcgcgcacaa
     8581 ccgtgcagga gtggaaaatc tttcgctcat tcccgggagc accggcgcgg gtccgatgca
     8641 gaacatcggc gcgtatggcg tggagctgaa agacgttttt cacggactgg aagcgttcga
     8701 cctgcgcgac ggaacgatgc ggaaattcac agccgaagaa tgccgtttcg gttaccgcga
     8761 aagcgttttc aagcatgagc tgcgcaaccg ctatttcatc acttcggtta cgttgcgcct
     8821 gaataaaaaa ccggtcttca acaccagcta cggcgcgatc gaaaaagagc tggagaaaat
     8881 gaaggtggag aaattatcca ttgccgcgat ctcgcaggcc gttatcaata tccgcagcag
     8941 caagcttccc gacccgaagc agaccgggaa cgccggaagc tttttcaaga accccgtcgt
     9001 gagcgcggaa aagcatgcac agctgaaaaa agatttcccg gaaattgttt cctacgcgaa
     9061 cgggaatgat ttcaagctcg ccgccggatg gctcatcgaa caatgcgggt ggaaaggaaa
     9121 acgtgccggc gatgcaggcg ttcataaaga ccaggcgctg gtgctggtga attacggtca
     9181 tgcgaccggg aaagaaattt tcgatctctc ttctgaaatt ctcgcttccg tgaaatcgaa
     9241 attcggcgtg gagctggaac gggaagtgaa tatttattaa caaagccggt gcgttaacag
     9301 aataatgtaa cgcgcttcac acgaaatttt atattttaat cgaatattgt acgcccgctg
     9361 cgggttttgc ccttccccgc agaatgcatt tcatttactt cctgcgcccg cattgtacaa
     9421 ttaacccatc gataaacaac caccttttat gaaaaaacaa tccaaccgcc agaggctggg
     9481 ctgggtgccc gaccttcccg atcaccgtga ccatctttac gccgcgccgc cgcacatcat
     9541 ctccaggctt ccctcgtcag tcgacctgcg gaaacaatgt ccgcccgtct acgaccaggg
     9601 cgatctcggc agctgcacgg cgaacgcgat cggcgcagcc atccagttcg agcagatgaa
     9661 gcagaaaaag aaacccttca tcccgtcccg tcttttcatt tattacaacg agcgtgcgat
     9721 ggagcacacg atcagctccg acagcggcgc gcagatccgc gacgggatca agagcgtgaa
     9781 caagcttggc gtatgccccg aaaccctgtg gccatacagc gacgataaaa cgcacttcct
     9841 gaaaaagccg gtcgccggtt gctacaagac tgcgctcgac aaccaggtgg tgtcgtacca
     9901 gcgcgtgacg cagacgctca tgcagctgaa aggatgtctc gccaacgggt acccgttcgt
     9961 tttcggcttc acggtttacg acaacatgta cagcgcagcg gtgacgaaaa cgggacatct
    10021 ctcgctgccc ggaccgaacg acaagatgga aggcggtcat gctgtgatgg ccgtcgggta
    10081 tgacgacagg aacgaacggt tcatcatccg caactcgtgg ggaagcaaat gggcgatgaa
    10141 aggatatttc acgctgccgt attcctacat caccgacagc aacctcgccg acgatttctg
    10201 gacgatccgc atggtggaat aaataccggg aaaagattcc ttcctgtttt ttatttggag
    10261 cgcaccacct cctcttctta ttcactttgc tcccgtgctc tcacgcagaa agcgtgatcg
    10321 cccggggcta aggaatattt ttcattgctc tgcatcacct gtttcaacta ccggcgtgca
    10381 gaagaattag gcgcatgcaa acgggatttt tttaggaacc ggttatgttc cgggctgcat
    10441 ttttccgccc accctcttca cttcttccca gaaacccggg taggattttt tcacgacacc
    10501 cgggttttca atgacgatgc cgggatgttt tatcgcgagc ggcgcgaacg ccatggccat
    10561 gcggtgatcg tcgtaagtag agatcgtcag cggagagact gtttttccat cgaaggaacg
    10621 tatatccagg agtcctgcag ccggttcctc gcatttcacg ccgatcttcg ccagctcatt
    10681 tttcagcgca gcaatacggt cagtctcttt cacgcgcaac gtgttgagcc cgcacatcag
    10741 cgtcgggatc ttcagcgcgg cggcggtgac agccaccgtc tgcacaatgt caggactgtc
    10801 ttcgaagtca aaagcgaaag cgttcgggcg gtaaccgttt ttcgttaacc gcacgccgtt
    10861 cgcttcgaac gtgcttgtca cgccgaggaa aggaaaaagc aatgcgcata ccgaatcggc
    10921 ctgcatgctg ttcttgtgca gcccgggaag aaaaatatcc gcttcatcgg cgagcgcaac
    10981 catgctgtac caataagaag cggcgctcca gtcgggttcg aggcggaact gctcttcctt
    11041 atcatcgacg aggtattgct ggctgctgac ggaaagcgtg tcgccgtccc ataccgggga
    11101 aacgccgaaa cgttccatga tgcgtatcgt catgtcgata tacggtcgcg acacaatggg
    11161 gcctttgaaa cggatgacaa tgcctttgtg catcgtcggt gcgatcatga gcagcgcgga
    11221 aacgaactgg ctgctcacgc ttccgtccat ttcaatctca ccgccggtaa gtggtccgcc
    11281 gaaaattttc agcgggggaa aaccttcttt ctccgggaat tcaatgtcgg cgccaagctg
    11341 ctgcaatgct ttcacaagta tcccgatcgg gcgctgcttc atgcgtgcag tgcccgtaag
    11401 atggaagacg ccgggtatcg aagaaagata tgcagagaga aaacgcattg ccgttccggc
    11461 aggacccaca tcgtacgtgt cgccgtcggt gtgcgtttcc agttgttcga gtatccggcg
    11521 tagcgagacc gtatcttccg cgtcggagat ctgcgggacc ggaactttcg tgccggtgat
    11581 ggcgcgtatc acgaggaggc ggttgcattc gcttttggaa agcggcagct tcacttcacc
    11641 tttaagcact ttcggaatgg acagggcgat acgttccata tattttcaat gtgactggtt
    11701 caggctggtg cggtaatacg caagcgaccg gagaatgccg gctgcgtcaa cgtttttatt
    11761 gatcaccggc tttcctatct catcgatcaa ggtaaaaaca attgaagcac cttcgttctt
    11821 cttgtcgtgc cgcatcagct cgatcagccg gtggtcagcc atcgcgtcga aaataacggg
    11881 aggatatttc gccaggatgg ttgccgtaat ttcctccatc gcttccccgg aaagcgaagc
    11941 gaacgtatgg gaaagaaaag cttcgcacac cattccggcg gcaactgctt caccgtgcag
    12001 cagcgtcgat tcggcgcgtt cgagaaaaaa agtttccagc gcatgaccga cggtgtgacc
    12061 gaaattcagc accctgcgca ggcccgcttc gtgcggatca gcttccacca cggaaagctt
    12121 gatgcgaacg gaacgaacga tcagctcttc ccaggaggaa gaatctgcag ggtgaacgtt
    12181 cttcagctcg ttccagtaat ccgcatcggc gacgaggccg tgcttcagca tttccgcaaa
    12241 gcccgaaaga atttcacgtc ccgggagcgt gcggagaaat tcaggataaa taaaaagttc
    12301 cgccggttcg gaaaacaaac cgatctcgtt cttgagcccg tcgagatcca cacccgtctt
    12361 accgccgacc gaagcatcga cctgggcaag aagcgttgtg ggaatgttga tgaacgtgat
    12421 cccgcgttta taagcagaag caacgaatcc gccgagatcc gtgatcacac cgccgccgag
    12481 attgatcagc agcgatttcc ggtcggcgcc cagctccgaa agcgcacgcc acacattcat
    12541 gcagacgtcc accgttttgt ttttttcacc gctttccagc tcgatgatct cggcttccgc
    12601 cagcattttc acacgggaaa caagcacagg gtagcaatgt tcaagcgtat gctcatcgac
    12661 aagaatgaaa acagacgaga acttgttttt tttcagaaag gaattcaggc tgtccgggac
    12721 gtactcgccg atgtgaacag gataacgggg tgacgcgatc gtgctgatct gccggatcat
    12781 tcacggcaaa gaaacgaaaa cggccgtgcc gggacaagtt tgtggcccgg taaattgtat
    12841 ttttgctcac cccgcgaaaa gcggaagaaa ccgattttta tgatctcgtt tgacaatacc
    12901 gaaatcgctt tttcaggaaa aacagacaaa gatctccggc gctcttactg gcttttcaaa
    12961 atgatcagca gtccggcgtt cgtgaatttc ggcaaggcga tgaccaattt tgcacttgct
    13021 attcacctgc cgatacgctg gatcatcaaa ccgacgatct tccgtcagtt cgtaggcggg
    13081 gaagacatcg cagaatgcag ccgcaccgtg aaggagcttg gcaagttccg catcggcacc
    13141 atcctcgatt attcggtgga agggaaagaa acggaagccg atttcgaccg cggcgcaaaa
    13201 gagatcatcg ccacgatcga gcgagcgaag aacgacgttc atattccttt ctgtgttttc
    13261 aaagtgacgg gacttgcccg tttcgcattg ctggaaaaag tcacctccgg gaaaacactt
    13321 accgcggaag aagaacagga atggaaacgc gttcacgggc gcgtcgaaaa tatctgcgga
    13381 acggccgcgt ctttcggaaa accaatcttc atcgatgcgg aagaaagctg gatccagcag
    13441 gcgatcgacg atcttgccga cgccatgatg cagctgttca acaaggaaaa atgcatcgtc
    13501 tacaatacgt tccagcttta ccggaaagac cgtctggatt tcctgcggaa gtcgtttgaa
    13561 aaagcgcagc agggaaatta tttcctcggg gcgaagctcg tgcgcggcgc gtacatggaa
    13621 aaagaaaggg cgcgtgcttc gcaaatgagc tatccctcgc cgatccagga aacgaaagcg
    13681 gacaccgacc gcgattacga cgaggcggtg aaattctgca tcgcacacgt cgaccgggtc
    13741 gggctttgtg ccggaacgca caatgaaaaa agttcacagc agctcgcgca actgctcgac
    13801 gaaaaaaaga tcacgcacga tcatccgcat gtttttttct cgcagctcct cggcatgagc
    13861 gatcatattt ccttcaatct ttcccatgaa gggtataatg tggcgaagta cgttccttac
    13921 gggcccgtgc gtgatgtgct tccctacctc atccgccgtg cgcaggaaaa tacgtcggtg
    13981 aaaggacaaa cgggacggga gctgaacctg atcatccgcg aaaaagagag aaggaaaggc
    14041 aggtagctgg tctcgactcg cgcccgacct gacaatagga tcgaccagtg tccgaccgcg
    14101 ggtgccgcgt gctgaaataa atgtgccggc gaaggcattt tttcatttcc gggcgcccga
    14161 ttccgggaat tcattttgta cctttagaac ttctcaaaaa acaccttttc ccttatgagc
    14221 aataccgccg caactgcagc gcgtgaactg aaagtcagtc acatggctga gtccctgatc
    14281 ggctctgaaa tcatcaagct ggccgccgac atcaatgaga aaatcaagaa cggcgaaacg
    14341 gtgtacaatt ttaccatcgg cgatttcaac ccgaagcttt tcccgatccc acaggagctg
    14401 aaaaaattca tcattgacgc gtataacaac gacgaaacca attaccccgc agcgaacggt
    14461 gtggccgagc tgcgaagcgc cgtctcgaac ctcattgaaa aacgccaggg actcaaatac
    14521 agtcctgacg aattccttat cgcgggcggc gcacgcccga tcatttacgc gatctaccgg
    14581 acgatcgtcg agaacgggga caaagtgctg ttcccggttc cctcgtggaa caacaaccat
    14641 tacacgcatc tctgcaccgg cgattatgcg aaccaggttt tcgttgaaac aaaaccggag
    14701 aacaatttca tgccgacggc agaagagctt cgcccgcacc tgtcgaaagt ttcgctcgtt
    14761 gccgtatgct ctccgcttaa cccgacggga accacgttca gtaaaaaaga cctcagcgag
    14821 atctgcgatc ttattctcgc cgagaatgca cgccgcggtc cgggtgaaaa accgctgtac
    14881 ctgctgtacg accagatcta ctgggcgctg acgtacggcg acacggttca ttacgacccg
    14941 gtttcgctcc gcccggaaat gcgcccgtac acgatcttcg tcgacggcat ttcgaaatcg
    15001 cttgcagcga ccggcgtgcg cgtgggctgg gcgatgggac cgaaagcgat catcgaccgg
    15061 atgaaatcca tcctttccca tgttggtgcc tgggcgccga aagcggaaca ggttgcgacc
    15121 gcaaaatttc tctccgatct ttccgcctat gataaattcc tcggcgaaat aaaacagaag
    15181 atcagcacga ggctgaccgc tttccataag ggattccagg acctgaaaaa gaaaggttac
    15241 gccgttgatt ccatcgcacc gcaggcagcc atttacctta cgatccgttt ttcgcttcac
    15301 ggcaaaaaaa caccggacgg aaaagtgctt gccaccacga aagacatcac cggctatatt
    15361 ctcaatgaag ctaaaatggc ggtcgttcct ttttacgcgt tcggcgcttc acctgattcg
    15421 agctggtaca ggctctcggt cggcacgtgc cggcccgaag acatcgaagc catcatgggc
    15481 agccttgaaa aagcgctcag caaactttcg tgaacggtcc gaaccatctc cttatgaaaa
    15541 aaaaccgttt ctgtgtgatc atggccggcg gcgtcggaac acgcttctgg ccgatgagcc
    15601 gttcgggaca tccgaaacag ttcatcgaca tactgggaac cggccagacg ctcctgcagt
    15661 ccaccttctc acgctcgctg cgcatctgcc cggcttcgaa tattttcgtc gtcacgaatg
    15721 aaaattattt cgatctcgtc cgcgaacagc tcccgaagct tccgccggaa aatatcctgt
    15781 gcgagccggc gcgaaaaaat accgcgccct gtgtcgccta cgcttcgtgg aagatccatt
    15841 ccatcgaccc ggacgcggtg atggtggttg ctccctctga tcacctgatc atgaaggaag
    15901 acacgtacgt gaaagcgatc aagtcgtgca tgcggattgc ggaaaacgaa gatcagctgc
    15961 tgaccatcgg catccgcccc acccgtcccg acaccggcta cgggtacata caattcgtga
    16021 acacagaaaa agaccggaag gacaaacgca tcaagaaggt gaaaacgttc acggaaaagc
    16081 ccgacctgga catggcgaaa ttcttcctgc agagcgggga tttcctctgg aactccggca
    16141 tcttcgtctg gagcgtcaaa agcgtgatga ccgcattcca ccgttactcg acggatcttt
    16201 tccagatctt cgaagaagga aaaggcctgt ggaacacgaa gaaggaaaaa aatttcctca
    16261 aagacgctta ttcccgctgc aagaatattt ccatcgatta cgccattctc gaaaaagccg
    16321 acaacgtgct cgtgcgcgca tccatcatcg gctggtcgga tctcggcaca tggggctcac
    16381 tctattccca cattcgcaaa gacgaccacg gcaatgcggt ggtgggaaaa caggtgatgc
    16441 tgtacaattc cggcaactgc gtcgtgcatg ttccgaaaga caagctcgtc gtgatcgatg
    16501 gactcgatga ttacatcgtc gtggaatcgg acaacatact tctcatctgc aaaaaacagg
    16561 acgagcagca gatccgcact ttcgtgaatg atgtcaagat cgccaagggc gaacggttcg
    16621 tatgaaaaaa ataattctgc ttcccgccat cgtttttttc ttctcctgcc acagcagccc
    16681 ggaaaaagtt tccccggacc cggtcggaac atggaaaccg cacgtgatgt gggacaacaa
    16741 cgacatcacg ctgcaggtgc ttcccgattc catgatcaaa ttcaaagcgg tgaaaggttt
    16801 ctgccccgga atcaaaacgt tcgtttccgt cggaaaatgg acgatcgtcc atgattcgat
    16861 cctgcagatg acgcagtaca ccgacgggcg caggtatacg atgaagcagc tgttcccgga
    16921 gctcgtgctc ccgaatgatt ccgtgatgcc cgacgtcatt ccgctggata tgaacgcgcg
    16981 gttcatcatc gcgaaagatc acctgtatga cgttgaactg gacgggaaga gatcggtgaa
    17041 acggtattac gaccggaaaa aataaaagtc cgcgatcaca tgtgatgcgg ctcttttcct
    17101 ttcacataac ctttgtaagc gccggaaaga taaatatcgg cacgcacgcc gatgatcgta
    17161 ttctgcttgt tgatattcgc aaaaagcgcg ccgccgccac cgacatcgaa gaataatttg
    17221 cggatgtact gcacctcgag ataaagcccc gtttccttgt acggatgaat attgtcgaac
    17281 gttcccgcat acaggtaagg atatcccgtt gaataggatg cgcccgccat catcgccagg
    17341 tggtacttga cctggtccgt acggtaaggt atccagcacg catgcgcctg gaaattattc
    17401 cacaacccga aaacgtcgcc gctgaacatc gtgccgagac ggaaataata ctgtttgatg
    17461 tgaaagttga aattcacacc gagcatcgtc tgcgtccgcg gatcgacgga atgataagcg
    17521 agtccgacac caccgctcac ccagctgttg taaacgcgga agcgtttgtt gttgatgacg
    17581 atctcttttt tgacgtcgac tgaatccttc tgcgcagcaa ggaaaagcgg aaaaaaaaga
    17641 aacaggaaaa tatagttcag tcgggacatt cggttacagg gtctgttgtc cgtaaagata
    17701 caaaggcgcg cattccgcat cccgcctcct gtcaaagagg aaaagccggt tgtgaaattt
    17761 ttcgccgccg gtttcagcag gagatcattt catcgatccg accatatctt ccggcctcac
    17821 ccattgatcg aactgttcgg gagttacata cccgagctcg atggccgttg cgcggagcgt
    17881 cttgttttcc ttgtgcgctt tcttcgcgat ctcggccgct ttgtaataac cgatatgcgt
    17941 gttgagcgca gtgaccagca tgagcgaatt ttcaagatgc tgccggatca cgggcaggtt
    18001 cggctgaatt ccgccggcgc atttttccgt aaacgaaacg cacgcatcac cgagcaggcg
    18061 cgcactctgg agaacgttgt acgcgatgag cggcttgaac acgttcagct cgaaatgacc
    18121 ggtagcgccg ccgatcgaaa ccgcgacatc atttcccatc acctgcgcgc acaccatcgt
    18181 cagcgcttcc ggctgcgtcg gattcacttt tcccggcatg atggatgagc ccggctcgtt
    18241 gtcgggaata ataatttcgc cgatgccgga acgcggacct gagctgagca tgcggatgtc
    18301 gttcgccact ttcatcagcg caactgccga gcgtttgagt gcgccggaaa gttccaccat
    18361 cgcatcatgc gcggcaagcg cttcgaattt gttcggtgcg gtgacgaacg ggaagccggt
    18421 gaactccgcg atctttttcg cgacgagcac gtcgtatcct ttcggcgtgt tgagtcccgt
    18481 tccgacggcc gttccgccga gggcgagctc acgcaccatt tcgagcgcat tcttcaccgc
    18541 gcggatgctg ttcgtgatct gctgcacata gccggaaaat tcctgcccga gcgtgagcgg
    18601 tgtcgcatcc atgaaatgcg tgcggcctgt tttcacgatc gaagaaaatt ccttcgcttt
    18661 tttatcgagc gcgtcacgca gcttttccat accgggaatt gtcacttcgc ttacgagctt
    18721 gtatgcggcg atgtgcatcg cggtcgggta ggtgtcgttc gacgactgcg atttgttgac
    18781 gtcgtcgttc gggttgagca ctttcttttc atccgtgagc tgtccgccgg aaatgacgtg
    18841 cgcgcggtac gcgatgacct cgttcgcgtt catgttgctc tgcgtgccgg aacctgtctg
    18901 ccagatgacg agcgggaact ggtcgtcgag tttgtgcgca agaatttcat cgcacacctg
    18961 ggagatgaga tcgcattttt ctttgggaag gacgccgagg tccatgttcg catgcgccgc
    19021 cgcttttttc aggtaagcga acgcgtagat gatctctttc ggcatactgg cttcgggccc
    19081 gatcttaaaa ttgttgcgtg aacgttcggt ctgtgcgccc cagtatttgt cggcgggaac
    19141 tttcacctcg cccatggtgt ctttttcgat gcggtagttc atggtggttg ttgtcatgcg
    19201 tattattgag ggatttgaaa tcggaagctc aatgcaaaaa tgccgaatta tcccgctcgt
    19261 tatcgcccgc cgaagtagtt tttatagaca taggcccggc tgtcgatgta accgtcgcgg
    19321 acctgtggag gcgcataccc gctgaaaagc gcaatgctgc aggaattttg atacgccgca
    19381 agatcgagga tcatgacgga gctgtcgggc atgcggagac cgatcttttt cttgtgcgtg
    19441 taatagagcg gcacgccgtt ttcccgcaac agtctttcct gctgctgttg aaccgccagc
    19501 cgctgcgatc tgacataagg aaactgccat tcaccgacga ggtttttcat cgcatggagc
    19561 tccgtcgtat cgggaacgta gcagtaggcg ccgacagcat ccaccagcgt atccggagtg
    19621 ccggtaacaa cgccgcgcga caaccacatg ccatagttca gttgcggaag gctgtgcgga
    19681 aggctgtagc ggacgctgtc ttcgcgcgaa aggatttcgt cgggcacggc gatgcttctt
    19741 gcgggattcg cgggatcaac cagcacaagg atgagctgct tcggcgtcat tccgccgttg
    19801 atgctgaacg gcgcatcgcg cttgtacagc tgcttgcggt aacggcggcc gttgtattcg
    19861 taatcgtaaa caatgcattt ctccgaagcg ccgcgaacgt aaccggctcg cagcgtgatc
    19921 agctcctgtt cgccgaagac cggattttcg agcagcagct tgctttcgta aggccgcagc
    19981 gtatcgggaa tcaatacaat gaacttatcg ccttccgcga gataaatatc ctcgtgctcg
    20041 acgccttcgt acatcttgcc gcgccattcg taacggtatt tcgtgtaatc gggattgctg
    20101 cccgaatgcg gactgttcac cgtagcagcc gccacagcga tatccgtcca gtcgaggttg
    20161 tgttcggcag catgaataac cgtagcaatg gtttccgctg gaacagcagg cggacgtgtc
    20221 gcaaccgtcg cgtgaggctg gtgacaggcg ccaagaaaaa aaggaaaaag aaacagcagg
    20281 aacagccgca tctccgaaag ttacggaacg ctcaggactc cacgaacgga atcacttttt
    20341 ctgtcggccg gccgatgatg gctttattgc ctttgatgat gatcgggcgt tcgatgagaa
    20401 tgggatattt caccatcgcc ttgatccatt tttcttccgt catctttttg cccgcgaatt
    20461 tctccttgta gatcggctct ttcttgcgga tgagttcttc ggcgggaatg ccgagtttct
    20521 tcaggatgtc gcggagctgc tcttccgtcg gcggcgttac gagatattcg aaaaccgacg
    20581 gttttacgcc ttcgtctttc agcaccttca tcacctcgca gctcttggcg caggtgtttt
    20641 tatgccagat ggtgatctta ctgtttccca ttttattcac cgtgccgcag ccggaaaatt
    20701 acataccgta aacttttcaa cttaccaacc tctcaacctc ccaacctttc tacgtcttca
    20761 attccatatt ccagctcgcg ccgtattcca tcaggtattt tttcacgaat tcattaagat
    20821 cgccgtcgag cacaccctgc gcgtccgacg tttcgtaatc cgtgcgcagg tctttcacga
    20881 gcttgtacgg gtgcagcaca tagttcctga tctgcgagcc ccattcgatc ttcttcatgt
    20941 tgccgcgaat ggcttgcacg gtttccatgc gtttccgcat ttcgatctcg tacagctgcg
    21001 atttcagcag gcgcatggcg ttctcgcggt tggtcatctg cgaacgcgat tcctggttct
    21061 tgatgatgat gccggtaggt ttgtgataaa gccgcacagc cgtttcgacc ttgttcacgt
    21121 tctgtccgcc ggcgccgccg gagcggaatg tttcccattc gcattcggaa ggattcactt
    21181 cgatctcgat cgtgtcgtcg atgagcgggt aaacgtaaac ggaagcgaac gaggtatgac
    21241 gtcttgcatt cgaatcgaac ggtgaaatgc gcacgaggcg gtgaacgccg ttctcgcctt
    21301 tgaggtagcc gaatgcgtaa tcgccttcaa tttccagcgt gcatgatttg atgccggccg
    21361 tttccccctg ctgcagatcg gcttccttga ctttgaaccc gttcttctcg ccccacatga
    21421 tgtacatgcg catgagcatg ctggcccagt cgc
//
LOCUS       DASTSF010000350         3494 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1136376,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000350 DASTSF010000000
VERSION     DASTSF010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3494
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1136376"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..247
                     /locus_tag="VFU15_09975"
     CDS             <1..247
                     /locus_tag="VFU15_09975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718153.1"
                     /translation="GSTPLPWETPYSANNALDPRNDPNSPFYDPSYETRNGANNFAFG
                     GAASTMGNGIRYGLVAYDNLINDIAHQIVDETKGLVK"
     gene            complement(275..784)
                     /locus_tag="VFU15_09980"
     CDS             complement(275..784)
                     /locus_tag="VFU15_09980"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU4718154.1"
                     /translation="MLRFESARLRFRDMEMRDTETMIRLNSDPEVIRYTGDPPFVSVE
                     SAHAFFAERIAAYKKHGYGRWITELKSSGEIIGWCGLKLHEDTGETDLGYRFFRAHWG
                     KGYATEGAKATIAFGCRTLGLTRIVAHARKENSASLRVLQKCGMKIIGEGVDCDGEIY
                     TWEIQKSRE"
     gene            complement(787..2028)
                     /gene="cysN"
                     /locus_tag="VFU15_09985"
     CDS             complement(787..2028)
                     /gene="cysN"
                     /locus_tag="VFU15_09985"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020529647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HEU4718155.1"
                     /translation="MDLLRFTTAGSVDDGKSTLIGRLLYDSKSIFEDQYEAVKASSEK
                     RGEEYVNLALLTDGLRAEREQGITIDVAYRYFATPKRKFIIADTPGHIQYTRNMVTGA
                     STANLAIVLIDARKGVIEQTMRHSFIASLLKIPHITVCVNKMDLVDYSRDVFEKICKD
                     FEAFASRLESIDINFIPISALNGDNVVDRSAKMPWYDGPTLLYHLEHMHIASDLNHID
                     CRFPVQYVIRPMSHEYHDYRGFAGRVAGGVFRKGDKVLVLPSGFSTKIKSIDLFGKEI
                     AEAFPPMSVTITLEDEIDISRGDMIVRENNQPEMSQDIDMMICWFNAKPLQPNGKYVI
                     RHTTREARCVVKEIQYKMDINTLHRIEDDKNIAMNDIGRIRMRTTVPLLFDKYSRNRI
                     TGSVILIDEATNETVGAGMIV"
     gene            complement(2085..2444)
                     /locus_tag="VFU15_09990"
     CDS             complement(2085..2444)
                     /locus_tag="VFU15_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006195273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HEU4718156.1"
                     /translation="MTKEQLKNRTKQFSIDVIRFVRDLPQGREADVIGKQLLKSATSV
                     AANYHAACRGKSKADFINKINIVEEEADESLFWLEIITETKMLLNDQNPMLLKEAGEL
                     TAIFTATGKTSKINNNK"
     gene            complement(2504..3409)
                     /gene="cysD"
                     /locus_tag="VFU15_09995"
     CDS             complement(2504..3409)
                     /gene="cysD"
                     /locus_tag="VFU15_09995"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013033368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HEU4718157.1"
                     /translation="MPNYTLKHLAELESESIFVLREVAAQFERPALLFSGGKDSIVVS
                     HLARKAFWPAKIPFPLLHIDTGHNFVETLEYRDWWANEIGAKVIVKYVQDSIDKGRAT
                     EEKGYNASRNALQTVTLLDAIEELKLDCAIGGARRDEEKARAKERFFSHRDEFGQWDP
                     KNQRPELWNLFNGRKNMGEHFRVFPISNWTEMDVWQYILQENIPLPKIYFTHKRKVIS
                     RDGQWLADSDFLVKKPTEVAEEKIVRFRTIGDMTCTGAVESTASTVEEIIQEVAASRT
                     TERGTRADDKRSEAAMEDRKKQGYF"
BASE COUNT          677 a          896 c          987 g          934 t
ORIGIN      
        1 gggctcgacg cccctcccct gggagacgcc gtacagcgcg aacaatgcct tggatccccg
       61 taatgatcct aacagcccgt tctacgaccc ttcttatgaa acgaggaacg gggcgaataa
      121 cttcgcattc gggggcgcgg caagcacaat gggaaatgga atcaggtacg gcctcgtcgc
      181 gtacgataac ctgatcaacg atatcgcgca ccagatcgtg gatgagacga aagggttggt
      241 caaataaaag tttttgctga atggagaaag ccggctactc ccggcttttt tgaatttccc
      301 aggtataaat ttccccgtca cagtcaactc cttcaccgat gatcttcatg ccgcattttt
      361 gcagcacgcg tagcgatgcg ctgttttctt tccgcgcgtg cgccacgatg cgtgtaagtc
      421 ctaacgtgcg gcacccgaat gcgatggttg ctttggcgcc ttccgtggca tagccttttc
      481 cccaatgcgc gcggaaaaaa cggtagccga gatcggtttc gcccgtgtct tcgtgcaatt
      541 tcagtccgca ccagccgatg atctctcccg aggatttcag ctccgtgatc cagcgaccat
      601 acccgtgttt tttatatgcg gcgattcttt ccgcaaaaaa agcatgagcc gattccaccg
      661 aaacgaaagg aggatcgccg gtgtagcgga tcacttccgg gtcgctgttg aggcggatca
      721 tggtttcggt atcgcgcatt tccatgtcgc ggaagcggag gcgtgcggat tcaaagcgca
      781 gcatgcttac acgatcattc ctgcgccgac ggtttcgttt gtcgcctcat cgatgaggat
      841 gaccgaaccc gtaatgcggt tgcggctgta tttgtcgaac agcagcggca cggtggtgcg
      901 catgcgtatg cggccgatgt cgttcatggc gatattcttg tcgtcctcga tgcggtgcag
      961 cgtgttgatg tccattttgt actggatctc tttcacgacg cagcgcgctt cgcgcgtggt
     1021 gtggcggata acgtatttcc cgttcggctg cagcggtttt gcattgaacc agcagatcat
     1081 catgtcgatg tcctgcgaca tttcgggctg gttattttcg cgcacgatca tgtcgccgcg
     1141 ggaaatgtcg atctcgtctt cgagcgtgat ggtgacgctc atcggcggaa acgcttcggc
     1201 gatctctttc ccgaaaaggt cgatggattt tattttcgtc gagaagccgc tcggcagcac
     1261 gagcactttg tcgcctttgc ggaatacgcc gccagccacg cggcctgcaa acccgcggta
     1321 gtcgtggtat tcgtggctca tcgggcggat cacatactgc acgggaaaac ggcagtcgat
     1381 gtggttgagg tccgacgcga tgtgcatgtg ctcgaggtga tagagcagtg tgggaccgtc
     1441 gtaccagggc attttcgcgg agcggtccac gacgttgtcg ccgttgagcg cggagatggg
     1501 aatgaaattg atgtcgatgg attcgaggcg cgaagcgaac gcttcgaaat ccttgcagat
     1561 cttctcgaac acgtcgcggc tgtagtcgac gagatccatt ttgttcacgc aaacggtgat
     1621 gtgcggaatt ttcagcagcg atgcgatgaa cgaatggcgc atcgtctgtt cgatgacgcc
     1681 tttgcgcgca tcgatgagca cgatggcgag gttggccgta gacgcaccgg tgaccatgtt
     1741 gcgcgtatac tggatgtgcc cgggcgtgtc ggcgatgatg aatttccgtt tcggcgtggc
     1801 gaaatagcgg tacgcgacgt cgatggtgat cccctgctcg cgttccgcgc ggagcccgtc
     1861 ggtgagcagc gcgaggttca cgtattcttc gccgcgtttt tcgctggatg ctttcaccgc
     1921 ctcgtactgg tcttcgaaga tcgatttgct gtcatacagc aatctcccga tgagcgtgga
     1981 ttttccgtca tcgacgctgc ccgcggtggt gaaacggagg aggtccattt ccaggtaaga
     2041 ttccgaagac atttcgggtt tcggatttcg gatttcggat ttgctcattt gttattgttg
     2101 attttagacg tttttcccgt ggccgtaaaa attgcggtaa gctcaccggc ttctttcaaa
     2161 agcatggggt tttgatcatt cagtaacatc ttagtttcgg taattatttc aagccaaaac
     2221 agtgattcgt cagcttcttc ttccacaata ttgattttgt tgataaaatc agcctttgat
     2281 tttccgcggc atgcggcatg gtagtttgcg gctacagaag tggcggattt taagagttgt
     2341 ttcccgatta cgtcagcttc tctgccttgc ggcagatcgc ggacaaaccg gatcacgtca
     2401 attgaaaatt gttttgtcct gttttttaat tgctcttttg tcataatcaa attagttgca
     2461 ttccaggttg gaaaatccga aatccgaaat ccgaaatccg aaatcagaaa tacccctgct
     2521 tcttcctgtc ttccatcgcg gcttcgcttc gtttgtcgtc ggcgcgggtt cctctttcag
     2581 tggtgcgtga ggcggctact tcctgtatga tctcttcgac ggtggaggcg gtcgattcta
     2641 cggcgcccgt gcacgtcatg tcgccgatgg tgcggaagcg gacgatcttt tcttccgcaa
     2701 cttccgtcgg ttttttcacg aggaaatccg aatcggcgag ccattgtccg tcgcggctga
     2761 tgactttgcg tttgtgcgtg aaataaattt tcggcagcgg gatgttttcc tgcaggatgt
     2821 attgccacac gtccatctcg gtccagttgg agatgggaaa cacgcggaaa tgctcgccca
     2881 tgtttttccg gccgttgaat aaattccaca attccggccg ctggttcttg ggatcccact
     2941 ggccgaactc atcacggtgc gagaagaagc gttctttcgc gcgcgctttt tcctcgtcac
     3001 gccgcgcgcc gccgatcgcg cagtcgagct tcagctcttc gatcgcgtca agcagcgtta
     3061 cggtctgcag cgcattgcgg ctggcgttgt agcctttctc ttcggtggcg cgtcctttgt
     3121 caatggaatc ctgcacgtat ttcacgatga cttttgcgcc gatctcgttc gcccaccagt
     3181 cgcggtactc gagtgtttcc acgaagttgt gaccggtgtc gatgtgcagc agcgggaacg
     3241 ggatctttgc cggccagaac gctttgcgcg caaggtgact gacgacgatg gaatcttttc
     3301 cgccggaaaa caggagtgcg ggacgctcga actgcgcagc gacttcgcgc agaacgaaga
     3361 tcgattccga ttccagctcg gcgaggtgtt tcagtgtgta attgggcatg ggatgggaag
     3421 gtctttttag ttttcggttt tcagttttat aaaacgggtc agcttatcca ggctttcttc
     3481 gaatgattct tcac
//
LOCUS       DASTSF010000351         3296 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1137217,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000351 DASTSF010000000
VERSION     DASTSF010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3296
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1137217"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2844
                     /locus_tag="VFU15_10000"
     CDS             <1..2844
                     /locus_tag="VFU15_10000"
                     /inference="COORDINATES: protein motif:HMM:NF025264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEU4718158.1"
                     /translation="VVPGRNNTNFTVVTPDAYQWTPSGTPVNYSWSPSTFLSSTTIAN
                     PMANNITATTTYTVTVDNNGCTASGTVTITAGSPLSATGSVSPAASVCTGTTVTLNST
                     PSGGGAPYTYSWTGPNSFTSTTQNPVITNATVADAGVYTCVITDNCTSTSTVNVTLTV
                     NPLPSVTVSPSTANFCNPGGTPVSLAAGGASTYVWTPSTGLSATTGANVTSSPAVNVV
                     YTVTGTDANGCVSTATASVTSSPAVTNAMATATPTAVCAGSPVSLNASSDPSPVTLVY
                     ENFNTGAPSWTRTNASTGGTPANAAWTDRPDGYVYAAGTPYHSNDNTQFVQTNSDAQG
                     SGGTTNATLRSPSFNTTGMTNIVVDFYQYYRDINDNGDSAVVEASPDGVNWTIVTAFT
                     ATTGSENSFTHPSVTLPVAFENQPAVYVRFRYIATWDWYWSIDNVTITGHSSNFTYSW
                     TSAPSGFTSSIQAPGDSPVIATTYSVQITNSYGCSATASVPVSINPLPVVTASASPSN
                     VVCQNDMVTFNGGGATSYSWSGGVTDNVPFSASSTGSYTVTGTDANGCSGTATLSLTV
                     NSLPVVTVSANPAAICAGDSSMLTGSAGGTSQWYMNGVAIPGATSSSYYTTMAGEYNM
                     TKTNINGCVDSASAGVMVSVNPLPVVSLGSDITQCGGTATLDAQNAGSAYLWNDNSTN
                     QVLTVSSTGNYYVDVTDANGCSASDSVMVTINALPVVSLGADTMVCGTSLTLDAQNAG
                     STYLWNDNSTNQVLAVSSNGTYYVDVTDANGCSGSDTIAVTINTPPAVMLSIPTDSTC
                     TNLAAFALSGESPSGGTWSGPGVTGSNFDPSAANLGFNMITYMFTDVNGCSGSASDSI
                     YNDICNGIPDAGVTHADIYPNPNSGQFTIRLSSVPVDPVKVEVMNSLGQRIDAFEMTS
                     TTRQVDLGIYSDGIYFVRITDGSTISTWRVVKQ"
     gene            complement(2898..>3296)
                     /gene="nadE"
                     /locus_tag="VFU15_10005"
     CDS             complement(2898..>3296)
                     /gene="nadE"
                     /locus_tag="VFU15_10005"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002656775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HEU4718159.1"
                     /translation="LYGDMCGGISVIGDVYKTQVYALSRFINREKEIIPASIIHKAPS
                     AELRPGQKDSDSLPDYAVLDKLLFEYIEKRKGPQELIDLGFDSALVNRVLKMVNTNEW
                     KRAQAPPILRVSPKAFGSGRRMPIVGKYLS"
BASE COUNT          744 a          945 c          854 g          753 t
ORIGIN      
        1 gtcgtacccg ggcgcaacaa taccaatttc actgttgtga cgccggatgc ataccaatgg
       61 actccttccg gaacaccggt caactattca tggtcaccgt cgactttcct gagcagcacg
      121 accattgcca acccgatggc caacaacatc acggctacga ccacttatac ggtaacagtt
      181 gacaacaacg gctgtacggc aagcggaacg gtaacgatca cagccggttc accgctttct
      241 gccacgggct ctgtatcacc tgcagcttct gtatgtacgg gcactacggt gacactgaat
      301 tcaacgccat caggcggagg tgcgccttac acgtattcat ggacaggccc caacagcttt
      361 acgtcaacaa cccagaatcc tgtcatcaca aacgcgacag ttgccgatgc aggtgtgtat
      421 acttgcgtaa ttacggacaa ctgcacgtct acatccacgg tcaatgtcac gcttacggtc
      481 aacccgctgc cttcggtcac cgtcagtcct tccacggcta atttctgtaa cccgggcgga
      541 actccggttt ctcttgcagc aggcggagca agcacgtacg tatggacacc atcaacaggt
      601 ctttctgcga ctacgggcgc gaacgtaact tcatcgccgg ctgtgaacgt ggtttacacg
      661 gttacgggaa ctgatgccaa tggatgcgtc agcactgcga cagcttctgt tacatcatcg
      721 cccgcagtta ccaatgcaat ggctaccgca acaccgacgg cagtctgtgc aggatccccg
      781 gtcagcctca acgcgtccag tgatccgtct cctgtaacgc tcgtgtacga gaacttcaat
      841 accggcgcac cgtcgtggac aagaacaaat gcatcgaccg gcggtacgcc tgcgaatgca
      901 gcgtggaccg atcgccctga cggatatgtt tatgctgcgg gaacacctta ccacagcaac
      961 gacaacacgc agttcgtaca gacgaacagc gacgcgcagg gaagcggcgg cacaacgaat
     1021 gcaacgctcc ggtcgccttc gttcaacacg accggcatga cgaatatcgt tgtcgacttc
     1081 taccagtatt atcgtgacat caacgacaac ggcgactcag ctgttgttga agcttctccc
     1141 gatggcgtta actggaccat tgtcacggca ttcaccgcta cgaccggttc ggaaaacagc
     1201 ttcacgcatc cttcggtgac gctgcctgtc gcttttgaaa atcaaccggc cgtctatgtg
     1261 cgtttccgtt atatcgcaac ctgggactgg tactggtcga tcgacaacgt aacgatcacc
     1321 gggcattctt cgaacttcac gtacagctgg acgtcggcgc cttccgggtt cacttcttcg
     1381 atccaggctc ccggcgattc acctgtaatc gcaaccacgt acagtgtgca gatcacgaac
     1441 tcctacggct gttcggcaac tgcaagcgtt cctgtcagca tcaaccccct tccggtcgtt
     1501 acggcttctg cttctccgtc gaacgtggtt tgccagaatg atatggtgac atttaacggt
     1561 ggcggcgcaa cgtcatactc gtggtcaggc ggtgtaacgg ataacgttcc gttctccgct
     1621 tcgtcaaccg gcagctatac cgtaaccgga acggatgcga acggttgctc gggtaccgcg
     1681 acgctttcgc tcaccgtgaa cagtcttcct gttgttaccg ttagcgccaa cccggcagcg
     1741 atctgcgccg gcgacagctc gatgcttacc ggctctgccg gcggaacgag ccagtggtac
     1801 atgaacggtg ttgccattcc cggcgcgacc agcagttctt attacacaac gatggccgga
     1861 gaatacaata tgacaaaaac aaatatcaac ggttgtgtgg attctgcttc cgccggggta
     1921 atggtttctg tcaacccgct gccggttgtt tcgctcggtt cggatatcac gcagtgcgga
     1981 ggaacagcaa cgctcgatgc acagaatgca ggatcggcct atctctggaa cgataacagc
     2041 acgaaccagg tgctgacggt ttcttcgacc ggtaattatt atgtcgatgt gacggacgcc
     2101 aacggttgtt cggcaagcga ttccgtaatg gtaacgatca acgcgctgcc ggtcgtttca
     2161 ctcggcgcag atacgatggt gtgcggaacc tcgctcacgc tggatgcgca gaatgcagga
     2221 tcgacctatc tctggaatga caacagcacg aaccaggtgc tcgccgtttc ttcgaacgga
     2281 acatactatg tcgacgtaac cgatgcgaac ggatgttccg gaagcgatac gatcgcggtc
     2341 acgatcaaca cgcctccggc cgttatgctt tctatcccga cggattcgac ctgcactaac
     2401 ctggctgcgt ttgcgctcag cggcgaatcc ccgtccggcg gaacatggag cggccccggt
     2461 gtgacgggat ccaacttcga tccttcggcg gcgaacctcg ggttcaacat gatcacgtac
     2521 atgtttaccg acgtgaacgg atgttccggt tctgcttccg acagcattta taacgatatc
     2581 tgcaacggta tcccggacgc aggcgttacg catgcggaca tctatccgaa cccgaacagc
     2641 gggcagttca cgatccggct ctcttccgtt ccggttgatc ccgtgaaagt ggaggttatg
     2701 aattcgctcg gccagcgtat cgatgcgttt gaaatgactt caacgacccg gcaggtggat
     2761 ctcggtattt attcggacgg aatttatttt gtccgcatca ccgatggatc cactatcagc
     2821 acgtggcgcg tcgtgaaaca gtaagtgtaa gaaagatgct caatgaaaaa tcccggaaat
     2881 ttttttccgg gattttttta tgaaagatat tttcccacga tcggcatccg tcttccgctg
     2941 ccgaatgctt tgggtgaaac gcgtagtatc ggcggagcct gcgcacgttt ccattcgttc
     3001 gtgttcacca ttttgagcac gcggttcacc agggcggaat cgaagccgag gtcgatgagc
     3061 tcctgcggac ctttccgttt ttcaatgtat tcgaaaagca gtttgtcgag cacggcgtaa
     3121 tcgggaagcg aatcggaatc tttctgcccg ggacgaagct ctgcagaagg cgctttgtga
     3181 attatcgatg cagggatgat ctccttttcc cggttgatga aacgggaaag cgcatacacc
     3241 tgcgttttat acacatcgcc gataacggaa attcccccgc acatgtcgcc gtacag
//
LOCUS       DASTSF010000352        15159 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_264926,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000352 DASTSF010000000
VERSION     DASTSF010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 15159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15159
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264926"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..4067
                     /locus_tag="VFU15_10010"
     CDS             <1..4067
                     /locus_tag="VFU15_10010"
                     /inference="COORDINATES: protein motif:HMM:NF019323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HEU4718160.1"
                     /translation="YYGKTVNRNILASDLGLIAKRGNDGSMLFAVNNLLTTDPVEGAQ
                     LEVYDFQHQLLTTTKSDEKGMARISLPKKKPFLLVAKNGDQRGYLKLDEGSSLSVSAF
                     DVGGQQVQKGLKGFIYGERGVWRPGDTLFLGFILEDKQNQLPLNHPVTFELNNSRGQL
                     VSRVVKAESVNGFYTFPTPTDPDAPTGYWSARVKVGGAVFTKDIRVEAIMPNRLKINL
                     DFGKQKFLTEEGDQTVKLDSRWLMGATAKNLKTQVDVSFYKTTTTFKGYDNYVFDDPA
                     TNFATENKTVFDGHLDENGTVSFDPKLSADNAPGKLRASFFTRVFEEGGAFSSDRFSI
                     DFSPYKYYVGLLVPKGSGWGGMLETGKDQGMRVVTVDENGNPVSREHLQVKVYKVDWR
                     WWWSDGYDDLAQYVTSDYYQPYYEQEISTKNGVGSFKLNVANEDYGRYLIRVTDDAGH
                     STGSLAWFDWPYWDGSGTGNNDIASLLSFSSDKKQYKVGDNINLTIPSPGQGRALISI
                     ENGSKIIDAFWAQTEKKGNITWTIPVTSEMAPNVYINVTLIQPHAQVKNDAPIRLYGV
                     IPIIVDDPKTHLTPMVSVPAVWKPEEQASVSVSEKDGKAMTYTIAVVDEGLLDLTRFQ
                     TPDPWGNFYAREALGVKSWDMYDMVMGAYGAEMGRVLGIGGDGDAGPKGGQKANRFKP
                     MVKFMGPFVLTPGERKTTTFMMPQYVGSVRVMVIAGQDNAYGNAEKTVAVRKPLMILG
                     TLPRVVGPGETVDLPVDVFAMEKKVKNVTVAVTKNSMFSVSGESTKTTTFSDIGDNIV
                     TFRLLVANSIGLGKVKITASGGGERAEYDIELDVRSPNPPMTDVTEAIIEPNQSWSSD
                     YTPNGIAGTNEGMLEVSTIPALNLGQRLNYLITYPYGCVEQTTSSVFPQLYLSDLMDI
                     DATKQSKIDNNIRAGIDRLRKFQTSSGGLSYWPGQNEPSEWGSNYAGHFLLEAQAKGY
                     TLPAGLIDGWKKFQKDQAVSWSPRYDTYYYGNDQLIQAYRLYTLALANAPELGAMNRL
                     REVPNLNVAAKWRLAAAYKLAGQQEIASQLVAGLSTTVAPYTELSWTYGSDQRDEAMI
                     LETLSLLGGANRIKGAPIAKSISEDLSNHSYWMSTQTTAYCLIALSKFSGTDKGNKGV
                     MCDYTIDGKAGKVNQSKPLAQVGMNIRGTQGGKITVKNNGSAVLFARIILTGVPAAGA
                     EKEEENNMGLTVQFSNLNGTPVDVSRIPQGDDFIATVTIYNPGVRGEYREMALTQIFP
                     SGWEIRNLRMEDSPDPVTSDPYDYQDIRDDRIYTYFWIGPRKTRTYRVLLNAAYSGHF
                     YMPATSCEAMYDHSINARRAGQWVDVVKSAGDIQ"
     gene            complement(4166..5299)
                     /locus_tag="VFU15_10015"
     CDS             complement(4166..5299)
                     /locus_tag="VFU15_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU4718161.1"
                     /translation="MSETQNPFPVNGYFGAELFCDREEETKRLVSNARNGVNTTLMAV
                     RRMGKTGLILHALASLERSRSTVCIYADVYATSSLKDFVNVLAAAILKTFPEKKSIGK
                     KFMTLLKSLRPVLSFDALTGEPEVSFDFADPKQYHHSLQSLFSFLEKQKVKIILAIDE
                     FQQVSVYPEQNTEARLRTIIQSLRNVQFIFSGSSPHLLHEMFSNSKRPFFSSTQPLYL
                     GSIRREKYAAFIVQQFARHRRKIRPDALEFILSWSRLHTYYTQALCNRVFASGERDVN
                     RETVNRACAELLGEQEMYFFQYRNLLTQAQWNLLTAIAKEERVYQPSAQNFIHRHRLG
                     TPSNVQRSLEALLAKEMIFRDKDAQGAYYTVYDCFLARWLEKN"
     gene            complement(5462..6781)
                     /locus_tag="VFU15_10020"
     CDS             complement(5462..6781)
                     /locus_tag="VFU15_10020"
                     /inference="COORDINATES: protein motif:HMM:NF040138.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HEU4718162.1"
                     /translation="MKTLNKIQAAAALLLTVNAFATAPPKTITLSVTENSNMTTQQVK
                     SMTFAFSTNGTDELDAYDSGNSTAISTDSNTVSPFSVTPDNDIISVTDTRFSLNGHKA
                     IPVGFATKIPATIKILATVGAVNPGDTIGLPAYVWIERISTGEVYSILGDTLKLDIPA
                     NDNFSSDFVLHTGTPVMNVASDESCFGALDGTIEVYNPGCNQWTLSLYDSNNALVQSF
                     PVYNPDTTIENLGSGTYNVVSKVGAVPVDSSTIVVNGPAEIIADFWIDNYNPFTSDVV
                     NFYNVSSGAVSYSWDFGDGNTDTSENASHQYLLPGNYGVVLTAMNASGCVQTVYDSVY
                     VTAPSSLMNGPGFDRSHTAADASNSQRDRNDMHVTVTTGEQKITVAQQDDAAPISVQV
                     LSMNGQLVASGVSSGDPVAFSVPQAGVYIVKIVSGGQMKSESVLVSQ"
     gene            7010..7786
                     /locus_tag="VFU15_10025"
     CDS             7010..7786
                     /locus_tag="VFU15_10025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718163.1"
                     /translation="MKHTYLRFLTALIFMTLAANLPGQGFGLPPGFDLKAFQKNEDQA
                     MWLVQYDSAWIHTASYDHLPQSRDYVCLPDKKGWKVVTGTLDSSGFRDAVYYSVDAKN
                     AVTRAQKQTDTALVNAMARALYNSNAALGKLNIKASGWRKFVKINADMTIAVWALCDA
                     DGSGNIWYGPECTWYYSSDGRKLTTSKIVNKAPLMAGKAGQTLNLSCPTDKMPTVGTM
                     FIAHKYRMDYTEITISYRTGSSTFRYDQSNKSYSWEHNAR"
     gene            complement(7792..8202)
                     /locus_tag="VFU15_10030"
     CDS             complement(7792..8202)
                     /locus_tag="VFU15_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020532290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU4718164.1"
                     /translation="MKKQKVQHNVVMLIDDNDIDNFINERMIRGCNFAETCYVNTSTM
                     SAIEFLKNLSVKKDLDANYLPSVIFLDINMPMLDGFQFIEEFEKLDKKITEPVSIVML
                     TSSINPADMERSKKYPSVKGFFHKPLTEEQLSSI"
     gene            8465..9871
                     /locus_tag="VFU15_10035"
     CDS             8465..9871
                     /locus_tag="VFU15_10035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718165.1"
                     /translation="MLKKVITQISDEDYSAFHAEISGNKAEKFTQLLELYRETDKDED
                     DIRKMVSGTPASFYTLKSRLFDKLRAYLFRTTADERGDLMKNISSLPKIANGLPRETA
                     ISLLEQLEAELKTFDMPRELISVYSALQRLHLNDDSYYQYQQLYNKTVAYALARDKSD
                     EVASLLNREICHFLLSADATQKEIIKLRLKELRNLSALYDSHRLKMNVWISSLTYALF
                     VDDHFEIPGTTETAEDLLGLAAETLKTHADVPYYHYVSMIWHFLNAEYYRRLGLKKNA
                     AESFGKVNAAVSSFLLLAHTCPASAFLLSRAERLGKDDEHISGFHPAPEDPFTMINVA
                     LHHASVDFHKGKYADAASMLNQLCNEVSFKNFPFAECQVKLFLGLSFALAEKSDQAEL
                     VVRSITRKLATEEYAGRFESAKQLGKFLKIAANDTSNSKKEKLEQAWQSFSLVNKGPE
                     AVLTFLKTGSEHIALLSK"
     gene            9918..11165
                     /locus_tag="VFU15_10040"
     CDS             9918..11165
                     /locus_tag="VFU15_10040"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEU4718166.1"
                     /translation="MNETSDHLQADMAQRDAAVVYNILVIAEHSNGIFPGDSFDGPQL
                     NITHARPSADLSGIAAAKSFDCIIADVFPSGKVTNADIATLKNGLPPGVPVIFLFDTL
                     PEKNIQASIYRSGCADIMAGPFDPAILAIKAETFARMHRQKKELHANLSEISRMNAML
                     REYRDEMDASIRYAKNIQDAILPKDQIIKAIFPQSFVFYRSKDIVGGDFYWLTVIRKK
                     AVVACVDCTGHGIPGALMSMIGNNLLKQIVEGKQLTDPSAILEEMNRGIRTTFSHNNI
                     SGHIVDGMEAAICTFDFTNDVLECAGARRHVLLFRRDGFEIIRPDPHGLGHGTTINPH
                     FTTHTFSVSPGDSVYMFTDGYTDQFGGPDEKRFMKKNLISLIMDNLAEDMTAQSGIVA
                     AAFDKWKGRQAQVDDVLVMGIRF"
     gene            11172..13880
                     /locus_tag="VFU15_10045"
     CDS             11172..13880
                     /locus_tag="VFU15_10045"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEU4718167.1"
                     /translation="MADYKKHKILLADDHSRDRSALKIVLQQNGFSVGEASSAADALH
                     MLAQESFGLILINADMPGADPAGFTEQLGARTKEQVPVVMISSTAPDLFFRSVPARGI
                     NDFLPKPVDKDLLLLKAHNLLSFFDTAEELRRSQEELNRMNLYLELKAIRSEISYKSL
                     YESSPDEIFILDKNGVVSKMNRDYVTSAGFDTKEYIGKPFAEAPCRFSLMNGESIVPL
                     LQLALAGKTGEDRTEIRMTGRDGNALFGEMTFGTFVTGSGEDQLQVIVRDITQRKLAE
                     IRLAEREQQLHEVTASIPGVVYQYKVFPGGKMEFTFVSEGSKKTWGLAPEEVYADSTA
                     PFRSIHPEDYERVMNAIDESNHSVRFLSVIFRIVMNGGKIKWLMAESVPHMTGDGILV
                     RNGSVTDITREKEARLLLEESERNFRTIVETTDQMINTTSTDGKILWANRAFLDATGY
                     SSDEITGMHIKDLLDESIRHFYPERARKVLSGTTIHGISGIMIRKDGERIFTDGTITP
                     LVENGVITGIRGFFLNVTEQKKAEELLRKNRERYKRVVDNISDGLVIDDPSGRIVFAN
                     DQFLHLFALTRNDLLQITLEDIVAPEYRQKIRDRHERRIAGENVPSVFEFRGLRKDGT
                     SGWFEVRVSPILENGQITGTQSAVRDITDDKNNLTELIRKEAELEKLVQDLTNKKNEL
                     TQFNYIVSHNLRSPIANIIGLTRLLEMTSLSEADRAKAIVHIRDSSHKLDEIVKDLSN
                     ILAARSGISSQKERLDIREIVRKASDILETQILESNCIINVNIAKNAGEIFSVKSYLE
                     SIIYNLLNNAIKYRAAGRRPEITVSSKKENGNIILAVADNGNGIDLERHGKDLFGLYK
                     RFNSEKEGKGLGLHMTKLQVETLGGKISVASTVGTGTTFTLTLPTG"
     gene            complement(13937..14899)
                     /locus_tag="VFU15_10050"
     CDS             complement(13937..14899)
                     /locus_tag="VFU15_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU4718168.1"
                     /translation="MEFKSDVEAVDFFRDRHGKLKKEIRKVIVGQEEVVEDVLISIFS
                     NGHCLLVGVPGLAKTLLVNTIAQALGLSYNRIQFTPDLMPSDIIGTEILDEGRQFSFV
                     KGPLFANIILADEINRTPPKTQSALLEAMQERGVTAAGKRYTLDKPFFVLATQNPIEQ
                     EGTYPLPEAQLDRFMFNVTLDYPSYEEEVEVVRSTTSDRTVQLGKVLGAEEILYLQDL
                     VRRVPVADNVLEYAVKLAGKTRPGTSMATADVNKFLSWGAGPRASQFLVLGAKCHAVV
                     TGKYSPDIEDVRAVAPPILRHRIVRNYKAEAEGVSVEAIIKGLF"
     gene            complement(14951..>15159)
                     /locus_tag="VFU15_10055"
     CDS             complement(14951..>15159)
                     /locus_tag="VFU15_10055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEU4718169.1"
                     /translation="ILYLKSRSQPHRANLKDDYQLIEDLALQKKQDDATDAWVRKKLA
                     TTYIRLDADYKKYKYRYPWLEFVN"
BASE COUNT         3784 a         4009 c         4054 g         3312 t
ORIGIN      
        1 attactacgg gaaaacggtc aaccggaata ttcttgcttc cgatctcggt ctcattgcga
       61 aaagagggaa cgacggaagc atgctctttg ccgtcaataa cctgctcacc accgatcccg
      121 tggaaggcgc acagctcgaa gtgtacgatt tccagcacca gctgctcact accacaaaat
      181 cggatgaaaa aggaatggcg cgcatttcac tgccgaagaa aaaaccgttc cttcttgttg
      241 caaagaatgg cgaccagcgc ggctacctga agctcgatga aggttcttcc ctttccgtca
      301 gcgcgtttga tgtcggcggg cagcaggtgc agaagggact caaaggtttt atttacggcg
      361 aacgcggcgt ctggcgtccc ggcgacacac tcttcctcgg cttcattctc gaggacaagc
      421 agaaccagct tccgctcaat cacccggtaa cgttcgagct gaacaattcg agagggcagc
      481 tggtttcgcg cgtggtaaaa gcggaatccg tcaatgggtt ttacacgttc cccacgccta
      541 ccgatccgga tgcgcccacg ggttactgga gcgcacgtgt gaaagtcggc ggcgcggtat
      601 tcacgaaaga tatccgcgtt gaagcgatca tgccgaaccg gctcaagatc aatctcgatt
      661 tcggaaagca gaaattcctt actgaagaag gtgaccagac cgtgaagctc gactcgcgat
      721 ggctgatggg cgcgaccgcg aaaaacctga aaacgcaggt cgatgtaagt ttttacaaaa
      781 ccaccacgac gttcaaagga tacgacaatt atgttttcga tgaccctgcg acaaatttcg
      841 caacggaaaa caaaacggtt ttcgacgggc accttgacga gaacggaacg gtatcgttcg
      901 atccgaaact ttccgccgac aatgcgccgg gcaaattgcg cgcatctttc ttcacccgcg
      961 ttttcgaaga aggcggtgcg ttcagcagcg accgtttctc catcgatttt tctccttaca
     1021 aatattatgt cgggctgctg gtgccgaaag gttccggctg gggcggaatg ctcgagaccg
     1081 ggaaagacca gggcatgcgc gtggtcacgg tggatgaaaa cgggaatccc gtttcacggg
     1141 agcatctcca ggtaaaagtg tacaaagtcg attggcgatg gtggtggagc gatgggtatg
     1201 atgatcttgc gcaatacgtc acgagcgatt actaccagcc gtattacgaa caggaaattt
     1261 ccacgaagaa cggcgtgggc tcgttcaagc tgaatgtcgc caatgaagat tacggacggt
     1321 atcttatccg tgttaccgat gatgccggcc actccacagg ttcgctggcc tggttcgact
     1381 ggccgtactg ggacggaagc gggaccggca acaacgatat cgccagcctg ctttcgttca
     1441 gctccgacaa aaagcagtac aaggtcggcg acaatatcaa tcttaccatt ccgtcaccgg
     1501 ggcagggacg cgcactgatc agcatcgaga acggatcgaa gatcatcgat gccttctggg
     1561 cgcagacgga aaagaaagga aacatcacgt ggaccatccc tgtcacttct gaaatggcgc
     1621 cgaacgttta tataaatgtc acgctcatac agccgcatgc ccaggtgaag aatgatgcgc
     1681 cgatacgatt atacggcgtg atacccatta tcgtcgacga tccgaaaaca cacctcactc
     1741 caatggtgag tgtgcccgcc gtgtggaaac cagaagagca ggcgtccgtt tcggtttctg
     1801 aaaaagacgg caaagcgatg acgtacacga tcgccgtcgt cgacgaaggt ttgctcgatc
     1861 tcacccgttt ccagacaccg gatccgtggg gaaatttcta cgcacgcgaa gcgctcggtg
     1921 taaaatcgtg ggacatgtac gacatggtga tgggcgccta cggtgcggaa atgggacgcg
     1981 tgctcggtat cggcggtgac ggtgatgcgg gaccgaaagg aggacagaaa gcgaaccgtt
     2041 tcaaaccgat ggtgaagttc atgggcccct tcgtgctcac tcccggcgaa agaaaaacga
     2101 cgacgttcat gatgccgcag tatgtcggca gcgtgcgtgt gatggtgatc gccgggcagg
     2161 ataacgcgta tggcaatgcg gaaaaaactg tcgctgtgcg caagccgctg atgatcctcg
     2221 gcacattgcc gcgcgtggtc ggaccgggag aaacagtgga tcttcccgtt gacgtttttg
     2281 cgatggagaa aaaagtgaag aacgttacgg tggcagtcac gaagaacagc atgttctctg
     2341 tttccggtga aagcacgaaa acgaccacct tcagcgatat cggcgacaac atcgttacgt
     2401 tccgtctcct cgttgccaat tccatcgggc tcggcaaagt gaaaatcaca gcatcgggcg
     2461 gaggcgagcg tgcagaatac gacatcgagc tcgatgtgcg aagtccgaac ccgcccatga
     2521 cagatgtcac ggaagcgatc attgaaccca atcaaagctg gtcatcggat tatacgccga
     2581 acggaatcgc gggaacgaat gaaggaatgc ttgaggtcag tacgatacct gcactcaatc
     2641 tcgggcagcg gctgaattac cttatcacct atccgtacgg ttgcgtggag caaactacgt
     2701 cttccgtttt tccgcagctc tacctttccg atcttatgga tatcgacgcg acaaaacagt
     2761 cgaagatcga caacaacata cgcgcgggca tcgaccgcct gcgcaaattc cagacctctt
     2821 ccggcggact ttcttactgg cccgggcaga atgaaccgag cgaatgggga agcaactatg
     2881 cagggcattt cctcctcgaa gcgcaggcaa aaggatacac acttcccgcc gggcttatcg
     2941 atggctggaa aaaattccag aaagaccagg ccgtgagctg gtcgccgcga tatgatactt
     3001 actattacgg aaatgatcag ctcatccagg cgtacaggtt gtatacgctg gcgctggcga
     3061 atgccccgga gcttggcgca atgaacaggc tgcgcgaggt tcccaatctc aacgtggcag
     3121 caaaatggcg gctcgccgcg gcgtataagc ttgccgggca gcaggagatc gcgagccagc
     3181 tcgtggcagg actttccacg actgtggcgc cgtatacgga actttcatgg acgtacggaa
     3241 gcgatcagcg cgatgaagcg atgatccttg aaacgctcag tcttctcggc ggcgccaatc
     3301 gcatcaaggg ggctcccatt gcaaaatcga tctctgaaga tctcagcaat cattcgtact
     3361 ggatgagcac gcagacgacc gcctattgcc tgatcgcatt gagcaagttc agcggaacgg
     3421 ataaagggaa caaaggagta atgtgcgatt atacgatcga cgggaaagcc gggaaagtga
     3481 accagtcaaa accgctggcg caggtgggca tgaatatccg cggcacacag ggcggaaaaa
     3541 taacggtgaa gaataacgga agcgcggtgc tcttcgcacg catcattctc accggtgtgc
     3601 cggcagccgg tgcggaaaaa gaggaagaga acaacatggg actcaccgtg cagttctcca
     3661 acctaaacgg gacaccggtt gatgtttcgc gaattcccca gggcgacgat ttcatcgcaa
     3721 ccgtcacgat ctacaacccg ggcgtgcgcg gcgaataccg cgagatggca ctcacgcaga
     3781 tattcccttc cggatgggaa atccgcaacc tgcgcatgga agacagcccc gatccggtca
     3841 ccagcgatcc gtacgattac caggacatac gcgacgaccg catttacacc tacttctgga
     3901 taggtccgcg gaaaacgcgc acataccgcg tgctgctcaa tgccgcttac tcggggcatt
     3961 tctatatgcc ggccaccagc tgcgaggcga tgtacgatca cagcatcaat gcgcggcgcg
     4021 ccggtcaatg ggtggacgta gtgaaatcgg caggcgatat ccagtaaccc cacgcctgcg
     4081 cctcatcccg gtctgcagta tgcattccgg gattttttca tttgtcgttt tcaattacgc
     4141 attttgcgtt acgcaaaatg cgtaatcagt ttttctccag ccagcgggcg aggaaacaat
     4201 cataaactgt atagtaagcg ccctgcgcat ccttgtcgcg gaagatcatt tccttcgcga
     4261 gaagcgcttc cagcgaacgc tgcacattgg aaggtgtgcc gagccggtgg cggtggatga
     4321 agttctgggc ggaaggctgg taaacgcgtt cttcctttgc aatcgccgtc agcaggttcc
     4381 actgggcctg cgtgagcagg ttgcggtact ggaaaaaata catttcctgc tcgccgagca
     4441 gttccgcgca ggcacggttc accgtttcgc ggttcacgtc acgttccccg gaagcgaaca
     4501 cgcggttgca gagcgcctgc gtgtaatacg tatgaagccg gctccaggaa agaatgaact
     4561 ccagcgcatc aggacggatt ttgcgccggt gccgggcgaa ctgctgcaca atgaatgcgg
     4621 cgtatttttc ccgccggatg ctgccgaggt acagcggctg cgtgctggaa aaaaaaggcc
     4681 gtttgctgtt gctgaacatt tcgtgcagca ggtgcgggct gcttccgctg aagatgaact
     4741 gcacgttgcg cagcgactgg atgatcgtcc ggagccgcgc ttccgtgttc tgttcgggat
     4801 aaacgctcac ctgctggaat tcatcgatgg cgagaatgat cttcaccttc tgcttttcca
     4861 ggaacgaaaa caggctctgc agcgaatgat ggtactgctt cggatctgca aaatcaaaag
     4921 agacttccgg ttcgccggta agcgcgtcaa agctgagcac cgggcgaagg ctcttcagga
     4981 gcgtcatgaa ttttttcccg atgcttttct tttccgggaa tgttttcagg atggctgccg
     5041 cgagcacgtt cacgaaatct ttcaggcttg aggtggcata tacatcggca tatatgcaga
     5101 ccgtgctccg tgatctttcc agcgacgcca gcgcgtgcag gatcagcccg gtctttccca
     5161 ttctccgcac cgccataagt gttgtattga cgccgttcct cgcgttgctg accagtctct
     5221 tcgtttcttc ttcccggtcg cagaaaagtt ccgccccgaa atacccgttt accgggaaag
     5281 ggttttgcgt ttcgctcata tttcaaatat acaaaatgcg ttacacattt tgcgtaacgc
     5341 aaaatgcgta atcattcctg tcgcgggagc cgggcacaaa aaacgtgtcg cggcattcct
     5401 gccgcgacac gcaaacacac acaaaacaca gagagagtcc ggggaaaacc cggaaagttt
     5461 tttactggct gaccagcacg gattcggatt tcatctgtcc gccggaaacg atcttcacga
     5521 tgtaaacgcc ggcctgcggt acggaaaagg caaccgggtc gccggaagaa acgccggaag
     5581 caacaagctg gccgttcatg ctgagcacct gcacgctgat cggggctgcg tcgtcctgct
     5641 gtgcaacggt gatcttctgc tcgcccgtcg ttactgttac atgcatatca ttcctgtcac
     5701 gctggctgtt ggacgcatcc gcagctgtat ggctgcggtc aaagccggga ccattcatca
     5761 gcgaagaagg cgcggtcaca taaactgaat catacaccgt ctgcacgcaa ccggatgcat
     5821 tcatcgcggt aagcacaacc ccgtaattgc cgggaagcag gtactggtgc gaagcatttt
     5881 cagaagtgtc ggtattaccg tcgccgaaat cccagctgta ggatacagcg ccggaagaaa
     5941 cgttatagaa attgaccaca tcgctggtga aaggattata attatcgatc cagaaatccg
     6001 cgatgatctc ggcaggcccg ttcactacga ttgtcgaaga atcaacggga acggcaccga
     6061 ctttcgatac tacattgtaa gtaccggaac cgaggttttc gatcgtcgtg tcaggattgt
     6121 agaccgggaa tgactgaacg agcgcgttgt tgctgtcata aagagagagg gtccactggt
     6181 tgcagccggg gttatatacc tcgatcgttc cgtcgagcgc accgaaacag gattcatcgc
     6241 tggccacgtt cattaccggc gttcctgtgt gcagaacgaa atcggaagaa aaattatcgt
     6301 tggccggaat atccagcttc agcgtatcgc cgaggatgga gtaaacttcc cccgtagaga
     6361 tgcgctcgat ccatacataa gcaggaagac cgatcgtgtc gccgggattt acggcgccta
     6421 cggtggcgag tattttaatg gtcgccggga tctttgttgc aaagcccacc gggatcgcct
     6481 tgtgaccgtt caggctgaag cgggtatcgg ttaccgagat aatatcgtta tcgggcgtaa
     6541 ccgaaaacgg tgaaaccgtg ttggaatccg tgctgatagc ggttgaattt cctgaatcat
     6601 aagcgtccag ctcgtctgtt ccgttcgttg aaaaggcgaa ggtcatcgat tttacctgtt
     6661 gcgtggtcat gttcgaattc tccgtaacgg aaagggtgat ggtcttcggc ggtgcggttg
     6721 caaatgcgtt caccgtgaga agaagtgctg cggctgcctg gattttgtta agggttttca
     6781 tggttctgtg ttttgtgttt tggtgttttg tttgttttac aggacaaacg tattgcagcc
     6841 agaacccggg agtaagccgt tcagatgcgc cttctttcgg atgtaagatt tccgtttttt
     6901 ttgtacagga agggtggagc cgggaaaacg ggaaataagc ccgcaaaaaa actatatttg
     6961 agaatggaac ggcacgttat ttgcctcgca taaggtccat ccttctgaaa tgaaacacac
     7021 ctacctacgc ttccttacag cactcatttt catgacatta gccgccaacc tgcccggcca
     7081 gggtttcggg ttgccgccgg gtttcgacct gaaagctttc cagaagaacg aagaccaggc
     7141 catgtggctc gtccagtacg acagcgcgtg gatccatacg gcgagctatg accacctgcc
     7201 gcagtcgcgc gattatgtat gcctgccgga taaaaaaggc tggaaagtgg ttaccggcac
     7261 gctggattcc tcgggcttcc gcgatgcggt ctattattca gtggatgcga aaaacgccgt
     7321 gacccgggcg cagaagcaaa cggatacggc gctggtgaat gccatggcga gagcgcttta
     7381 taattcgaat gcggcccttg gcaagctgaa tataaaagcg agcgggtgga gaaaattcgt
     7441 gaagatcaat gccgacatga ctattgccgt gtgggcgctg tgcgatgcgg atggcagcgg
     7501 gaacatctgg tacggccccg aatgcacatg gtattattcg tccgacggaa gaaagctcac
     7561 gacttcaaag attgtcaaca aggctccgct catggccggg aaagccgggc agacgctcaa
     7621 cctgtcctgt cccaccgata aaatgcccac cgtgggaacc atgttcatcg cccataaata
     7681 caggatggat tacaccgaga tcacgatcag ctacaggacg ggctccagca cgttccgcta
     7741 cgaccagtcc aataaatcgt attcctggga acacaacgcc cggtaagttc ttcagatcga
     7801 agacagctgc tcttccgtca gcggcttgtg gaaaaagcct ttcaccgaag gatatttttt
     7861 tgaccgctcc atatcggcgg gattgatgga gctcgtaagc atgacgatgc tcaccggttc
     7921 ggtgatcttt ttgtccagct tttcgaattc ctcgatgaac tggaacccgt cgagcatggg
     7981 catattgatg tcgagaaaaa tgacggatgg caggtagttc gcatccaggt cctttttcac
     8041 cgaaaggttt ttcaggaact cgattgcgct catcgtgctc gtattcacat agcacgtttc
     8101 cgcaaaattg cacccgcgta tcatccgctc attgatgaaa ttatcgatat cgttatcatc
     8161 gataagcatg actacattgt gctgcacctt ctgcttcttc attttccgtc ttgtcctttt
     8221 ttttcgaaaa tataaacatt tgatgaatta ataaaatccc cagttgattt atgcgaaaat
     8281 caggtttcat taagaatgtg gtaacgcgta tattagcccg gacataaccg atcccggccc
     8341 ggattgcctg ccttccatcc ccctcgaacg gaagagtgct gccggcccgg atcaaatgga
     8401 aataaaaaac atccgggtac cgttggcggg acccattccc ggcaaaaaat acagaagtgt
     8461 atctatgctc aaaaaggtaa tcacccagat cagcgatgaa gattattccg cttttcacgc
     8521 tgaaatttca gggaacaagg ctgaaaaatt cacgcagctg ctcgagctct accgggaaac
     8581 cgacaaggac gaggacgata tccggaaaat ggtgagtggc acgcctgcct ctttctatac
     8641 gctcaagtcg agactgtttg acaagctgcg cgcgtatctt ttccgcacca ccgctgacga
     8701 acgcggcgac ctgatgaaaa atatcagctc gctgccgaag atcgccaacg ggttgccgcg
     8761 cgagaccgcc atttccctgc tggagcagtt ggaagcggaa ctgaagacat tcgacatgcc
     8821 ccgcgagctc atcagcgtgt acagcgcgct gcagcggctt cacctgaacg acgattctta
     8881 ttaccagtac cagcagctgt acaacaagac tgttgcctac gcgctcgccc gcgacaaaag
     8941 cgatgaagtg gcgtccctgc tcaaccgcga gatctgtcat ttcctgcttt cggcggacgc
     9001 tacgcaaaaa gaaataatca agctgcggct gaaagagctt cgcaacctca gtgcgcttta
     9061 tgattcgcac cgcctgaaaa tgaacgtgtg gatctcttca ctcacctatg ccctgtttgt
     9121 cgatgatcat ttcgaaatac cgggaacaac ggaaacggca gaagaccttc tcggcctcgc
     9181 tgcggaaacg ctgaaaacgc acgcggacgt tccctactac cattacgttt ccatgatctg
     9241 gcattttctg aatgcggaat actaccggcg tctcggcctg aaaaaaaacg ccgccgaatc
     9301 attcgggaaa gtgaacgcgg ccgtcagctc attccttctt ctcgcgcaca cgtgtcctgc
     9361 ttccgcgttc ctgctttcac gcgcggaacg tctcggcaag gacgatgagc acatctccgg
     9421 ttttcacccg gccccggaag atccgttcac gatgatcaat gttgcgctgc accatgcctc
     9481 cgtcgatttt cacaaaggga aatacgccga cgccgcctcc atgctcaacc agctgtgcaa
     9541 cgaagtaagc ttcaagaatt ttccgttcgc ggaatgccag gtcaagctct ttctcggcct
     9601 ttcgttcgcg ctcgctgaaa aaagcgacca ggcggaactc gtcgtgcgga gcattacgcg
     9661 aaagctggcc acggaagaat acgcgggcag gttcgaaagc gcgaagcagc tcgggaagtt
     9721 cctcaagatc gctgcaaatg atacgagcaa ttcaaaaaaa gaaaagctgg aacaggcgtg
     9781 gcagagcttt tcgcttgtca ataaaggccc ggaagcggtg cttaccttcc tgaaaaccgg
     9841 cagtgagcat attgcgctcc tgtcaaaatg agccgggaca aaaccaggtc agcagaacga
     9901 ataaaaccac gaaacacgtg aatgaaactt cagatcatct gcaggcggat atggcgcaac
     9961 gcgatgctgc cgttgtgtac aatatcctgg tgatcgcgga acacagcaac ggtatcttcc
    10021 cgggagattc cttcgatggt ccgcagctga acattacaca tgcccggcct tcggcggacc
    10081 tcagcggcat cgccgcagca aagagcttcg actgcatcat cgcggatgta tttccctccg
    10141 gcaaggttac gaatgcggac atcgctacgt tgaaaaacgg gcttccgccc ggcgtcccgg
    10201 ttatttttct tttcgacacc ctacctgaaa aaaatataca ggcctccatt taccgttccg
    10261 gctgcgccga tatcatggca ggtcctttcg atcctgctat tcttgccatc aaagccgaaa
    10321 cattcgcacg catgcacagg caaaagaaag agctgcacgc gaatctttcc gagatcagcc
    10381 gcatgaacgc catgctccgc gaataccgcg acgagatgga cgccagcatt cgctacgcca
    10441 aaaatatcca ggacgcgatc cttccgaaag accagatcat caaggccatc tttccgcagt
    10501 cgttcgtctt ttacaggtcc aaggatatcg tgggcggaga tttttactgg ctcaccgtca
    10561 tccggaaaaa agccgtcgtc gcctgcgtcg attgcaccgg gcacggaatt cccggcgcgc
    10621 tcatgagcat gatcggcaac aatctgctca agcagatcgt cgagggaaaa cagctcaccg
    10681 atccatccgc gatcctcgaa gaaatgaacc gcggtatacg cacgaccttc agtcataaca
    10741 atatcagcgg gcacatcgtc gacggaatgg aagcggcgat ctgcacgttt gattttacaa
    10801 atgatgtgct cgaatgcgcg ggagccagga gacatgtgct tctcttccgc agggatggtt
    10861 tcgagatcat acgtcctgat ccgcacgggc tcggtcacgg cacaacgatc aacccgcatt
    10921 tcaccacgca cacattcagc gtttcaccgg gcgattccgt ttatatgttc acggatggat
    10981 acacggacca gttcggcggt ccggatgaaa aacgtttcat gaagaagaac ctgatcagcc
    11041 tcatcatgga taacctcgcc gaggacatga cggcgcaatc cgggatagta gcggctgctt
    11101 tcgataaatg gaaaggccgg caggcccagg tagatgacgt gctcgtgatg ggaatacgct
    11161 tctgattatt tatggctgat tacaaaaaac ataaaatact tctcgcagac gatcatagtc
    11221 gcgaccgttc cgccctgaaa attgtacttc agcaaaatgg gttttctgtc ggggaagcgt
    11281 cttccgcggc ggatgcattg cacatgcttg cgcaggaatc cttcgggctg atactgatca
    11341 acgcggacat gcctggtgca gatccggcag gctttacgga acagctgggc gcacgtacga
    11401 aggagcaggt tcccgttgtc atgatttctt ccacggcgcc ggatcttttt ttccggtcag
    11461 ttcccgcccg gggcatcaac gattttcttc cgaaaccggt cgacaaggac ctgcttttgc
    11521 tgaaagccca taatctcctt tccttttttg atacggccga agagctccgc aggtcgcagg
    11581 aagaactgaa ccggatgaat ctctatctcg agctgaaagc tatccgctcg gagatctcct
    11641 acaaatcgct gtatgaaagt tccccggatg agatatttat tctcgacaag aacggcgtgg
    11701 tcagcaaaat gaaccgcgac tacgttactt ccgccgggtt cgacacgaag gaatatatcg
    11761 gaaaaccatt tgcggaagcg ccgtgccgct tttcgctgat gaacggcgaa agcattgtcc
    11821 cgctgcttca gctggcgctg gccgggaaaa ccggtgaaga ccgcaccgag atcaggatga
    11881 ccggccggga tggcaacgcc ctcttcgggg aaatgacatt cgggacattt gtaaccggca
    11941 gcggcgagga tcagctgcag gtgatcgtcc gcgatataac gcagcgaaag ctcgccgaga
    12001 tccgtctcgc ggaacgtgaa cagcagctgc acgaggtaac ggcttccatc ccgggtgtcg
    12061 tgtaccagta taaagttttt ccgggcggga agatggaatt cacattcgtc agcgaaggtt
    12121 cgaaaaaaac gtggggcctc gcacccgaag aagtgtacgc ggacagcact gccccgttca
    12181 ggagcattca cccggaagat tacgaacgcg tgatgaacgc cattgacgaa tcgaaccatt
    12241 ccgtcaggtt tctttcggtg atattccgta tcgtaatgaa cggcgggaag atcaaatggc
    12301 tgatggcgga aagtgttccg cacatgaccg gcgacggcat ccttgtgcgc aacggaagtg
    12361 tcaccgatat tacccgcgaa aaagaagccc ggcttctgct ggaggaaagc gaacggaatt
    12421 tccgcaccat agtggaaacc acggaccaga tgatcaatac tacatcaacg gatggaaaga
    12481 tactctgggc taaccgggcg tttctcgacg caaccgggta cagcagcgat gagatcacgg
    12541 gaatgcacat aaaagacctg ctcgacgaaa gcatccggca tttctatcct gaacgtgcgc
    12601 gcaaggtttt gtcgggaaca acgatccacg gaatttccgg catcatgatc cgcaaggacg
    12661 gcgaacgcat attcaccgac ggaacgatca caccgctcgt ggagaacggc gtgatcaccg
    12721 gtattcgcgg cttttttctc aacgtcaccg aacagaagaa agcggaagaa ctcctccgca
    12781 aaaaccggga acgctataaa cgcgtagtgg ataacatcag cgacgggctc gtcatcgatg
    12841 atccctccgg caggatcgtt ttcgccaacg accagttcct gcacctgttc gcgcttacaa
    12901 gaaatgatct tctgcaaatc acactcgaag atattgtcgc accggaatac cggcagaaaa
    12961 tcagggaccg gcatgaacgg aggatcgccg gggaaaatgt tccttccgtt tttgaattcc
    13021 gcggattgcg caaagacgga acatccgggt ggttcgaagt gcgcgtttcc cctatcctgg
    13081 aaaacgggca gatcaccgga acacagtcgg ccgtgcgcga tataacggac gacaagaaca
    13141 acctcacgga gcttatccga aaagaagcgg aactggaaaa gctcgtgcag gatctcacca
    13201 ataaaaagaa cgagctcaca cagttcaatt acatcgtctc ccacaacctg cgttccccca
    13261 tcgcgaacat catcggtctt acacgcctgc tcgaaatgac ttcactgagc gaagccgacc
    13321 gtgcaaaagc catcgttcac atccgcgact catcacacaa actcgatgaa attgtaaaag
    13381 atctcagcaa tattctcgcc gcccgctccg ggatcagttc gcagaaagag cgtcttgata
    13441 tccgggagat cgtacggaaa gcatcggaca tactggaaac acaaatactg gaaagtaact
    13501 gcatcattaa cgtaaacatt gcgaaaaatg cgggcgaaat attttccgtg aagtcatacc
    13561 tcgaaagcat catctataac ctgctcaaca atgctatcaa atacagggcc gcggggcgaa
    13621 ggccggaaat aactgtttct tcaaaaaagg aaaacgggaa tatcattctt gctgttgccg
    13681 acaatggcaa cgggatcgac ctggaacggc acgggaaaga tcttttcggc ttatacaaac
    13741 gcttcaattc cgaaaaagag ggaaaaggac tcgggctgca catgacgaag ctgcaggtgg
    13801 aaacgctcgg gggaaaaatt tcagtcgcga gtaccgtggg aacgggaaca acgttcacgc
    13861 ttacactgcc caccggttaa agctccgtgc tttgcacggc tttcccaaaa acacttcccg
    13921 gagtgcggcg gaaaaatcag aaaagtcctt tgatgatcgc ttcaacgctg acaccttccg
    13981 cttctgcttt atagttgcgt acgatgcggt gacggaggat cggcggtgcc actgcgcgca
    14041 catcttcgat atccggtgaa tatttcccgg taacgacggc gtggcatttt gcaccgagca
    14101 cgagaaattg cgacgcgcgc ggacctgcgc cccacgaaag aaatttattt acatcggcgg
    14161 tggccatgga agttccgggt cttgttttcc cggccagctt cacagcatat tcgagcacgt
    14221 tatctgcaac ggggacacgg cgcacaaggt cctgcaggta aaggatctct tcggcgccga
    14281 gcactttccc cagctgaacg gtgcggtcgg atgtcgtgct gcgcaccact tccacttctt
    14341 cctcgtagct gggataatcg agcgtgacgt tgaacatgaa acggtcgagc tgcgcttccg
    14401 gcagcgggta cgttccttcc tgctcgatcg ggttttgcgt ggcaagaacg aagaaaggct
    14461 tgtcgagcgt gtagcgtttt cctgccgcgg ttacgccgcg ttcctgcatc gcttcgagaa
    14521 gagcggactg cgttttcggc ggtgtacggt tgatctcgtc ggcgagaata atattggcga
    14581 acaagggtcc tttcacgaaa ctgaactgcc ttccttcatc gagaatttcc gttccgatga
    14641 tatccgaagg catgagatcg ggcgtgaact ggatccggtt gtaggaaagt ccgagcgcct
    14701 gcgcaatggt attcacaaga agcgttttgg caaggcccgg cacaccgacg agcaggcaat
    14761 gcccgttgga gaaaattgag atcagaacat cctccaccac ttcttcctgg ccaacgatca
    14821 ctttccgtat ttcttttttc agctttccgt gacggtcgcg gaaaaaatct actgcttcaa
    14881 cgtcggattt gaattccatg tggttctgat tttctgaatg aggttttaca aaaggagctt
    14941 catcccgaaa ttaattaaca aactccagcc aggggtaacg gtacttgtat tttttgtaat
    15001 cggcatcgag acggatatag gttgtggcga gcttcttccg cacccacgcg tcagtcgcat
    15061 catcctgctt tttctgcagc gcaagatcct cgatgagctg gtaatcgtct ttcaggttgg
    15121 cccggtgcgg ctgcgaacgc gatttgagat aaagtatgc
//
LOCUS       DASTSF010000353         2546 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1440242,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000353 DASTSF010000000
VERSION     DASTSF010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2546
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1440242"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="VFU15_10060"
     CDS             <1..315
                     /locus_tag="VFU15_10060"
                     /inference="COORDINATES: protein motif:HMM:NF025734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding domain-containing protein"
                     /protein_id="HEU4718170.1"
                     /translation="HYPWYAEVQPVGWWLANHVSDGREELNFDEFGTYSPRRRFWKLN
                     GIIKQLDEDEMPLSSYTLIHRDAILTPETKTLIVNWAKAMQDTMKNHYPADSLSKPQR
                     KK"
     gene            686..>2546
                     /locus_tag="VFU15_10065"
     CDS             686..>2546
                     /locus_tag="VFU15_10065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718171.1"
                     /translation="MKKTLRSILLAVPASLLLLPSTVNAQCSGGTLAGNIVPTTTIQT
                     VTVPSGGSYFTFTGVAGATYMFSFCASEGGSASFDTQLTILDDATGNYAGGYNDDWCG
                     LQSYLTWACPSNAVYRVLASQYYCTTNATSATMAYKIMPPPNDDCVNATYIGVPSTVV
                     GNTIYANPDVAPTCTTSDAAGGGVWYEVMGDGNQMTASLCGSSFDTRLRIFSGSCGAL
                     VCEGGNDDFCATQSQVTWCSVNGTLYYILVHGNGAATGDFTLAMSEVYVQPPFVNPSV
                     TAFCGSGTVTINASGSTVYNWSPAIGLDTTGGPTVVATPPSTTTYTVAITDAVTGCPA
                     YNTAQVIINPIPVVTASASSTLICGGGSSTLTGGGASSYTWMPGNLTGTSVTVTPTVT
                     TTYTVTGVDGNGCSDTASVTVNVYPIPPVASTSAPASICIGDFATLSASGANTYSWMP
                     GNLTGQNVNVAPTTTTTYTVTGTDGNGCTATSNYTLVVHPLPAITATAAQYTICEGSP
                     SSLMGSGGVSYVWSDNSTANPDTTYPNLTTTYTVTGTDTNGCSNTDTVMVNVLWAPPV
                     SATASVPTICSSGGSVLTASGSQSYTWMPGSMTGSSITVTPSSTTTYTVTGMNS"
BASE COUNT          601 a          736 c          645 g          564 t
ORIGIN      
        1 cattatccgt ggtatgcaga agtgcagccc gtcgggtggt ggctggcgaa tcacgtcagc
       61 gacggcaggg aagagctcaa tttcgatgag ttcgggacgt acagcccgcg gcgccggttc
      121 tggaagctga acgggatcat caaacaactc gatgaagatg aaatgccgct gagctcgtac
      181 accctcattc accgtgacgc cattctcacg cccgaaacca aaacgctgat cgttaactgg
      241 gcgaaagcga tgcaggatac gatgaagaac cattacccgg ccgacagtct ttcaaaaccg
      301 cagcggaaaa aataatcgcg gcagcccggc tctatattat ttttctcgcc gaacaacggt
      361 ctgcgcagga acgcccgtta aaaaacacgc tggaaaaagg acggcacact gcatcgcgat
      421 cattaaaaaa ataaagagcg gattagaatc ccggggactg tttttcttcc gccgtttact
      481 atcgggggga ggttccgatg cggccggtcc gttttttaac gcatgtggaa ttgtttggac
      541 agcaatacga tacgaaacga aaaacatcgg gcagaaagca ttttatgcgt ggatcgttaa
      601 tgaaaacagg aaaattacag cgccgggcat tctcgccgct ccgaatatat tttctaattt
      661 taatgaaaat ctaattgtgg caaaaatgaa gaaaacacta cgctcaattt tactggccgt
      721 tccggcatcc ctgctccttc ttccctcgac cgtcaatgcg caatgttcgg gaggaacgct
      781 ggcaggaaat attgtaccga ccacaaccat acaaaccgtg acggtaccgt cgggcggatc
      841 ctatttcact tttaccggtg ttgcaggcgc cacctatatg ttttccttct gcgcgtcaga
      901 gggcggctcc gcatcctttg atacgcagct cacgatcctc gacgacgcta ccggtaacta
      961 tgccggcggc tacaatgacg actggtgcgg cctgcagtcc tacctcacct gggcctgccc
     1021 ttcgaacgcg gtttaccgcg tgctggcttc ccagtattat tgcacgacaa acgccacctc
     1081 ggcgaccatg gcttacaaga tcatgccgcc gccgaacgac gattgcgtga atgcaaccta
     1141 catcggcgtt ccttcaacgg ttgtcggcaa tacgatttat gccaacccgg atgttgcgcc
     1201 cacgtgtaca acttccgacg ctgcaggcgg cggtgtgtgg tacgaagtga tgggcgacgg
     1261 gaaccagatg actgcttcac tttgcggttc cagcttcgat acacgtctgc gcattttcag
     1321 cggaagctgc ggcgcacttg tctgcgaagg cgggaatgac gatttctgcg ccacgcaatc
     1381 acaggtaacc tggtgttccg tcaacggcac attgtattat atccttgttc atggcaacgg
     1441 cgctgccacc ggtgacttca cgctggccat gtctgaagta tatgtgcagc ctccgttcgt
     1501 taacccgtca gtaacggcgt tctgcggatc aggcacggtc acgatcaatg cttcgggttc
     1561 taccgtttat aactggtcgc cggcgatcgg gctcgataca acgggcggac cgacagtagt
     1621 tgcgacaccg ccgtcaacca ccacgtacac tgttgcaatc actgacgcag tcaccggttg
     1681 ccctgcttat aataccgcac aggtgatcat caaccccatc ccggttgtga cggcaagcgc
     1741 ttccagcacg ctcatctgcg gtggcggcag cagcacgctt acgggaggcg gcgcttcaag
     1801 ctatacgtgg atgcccggca accttaccgg aacaagcgtc acagttacac ctacggtgac
     1861 gacgacctat accgtaaccg gggtcgacgg caacgggtgt tcagatactg catcggttac
     1921 cgtaaacgtt tacccgatcc cgccggtagc cagcacttcg gctcccgcat ccatctgcat
     1981 cggagatttt gccacgcttt ccgcttccgg ggccaataca tattcctgga tgcccggtaa
     2041 ccttacagga cagaacgtga acgttgctcc gacaacaact acaacttata cagtaaccgg
     2101 aaccgacggc aatggctgta cggcgacatc caattataca ctcgttgtac atccgctgcc
     2161 ggcaatcact gcgaccgctg cacaatacac gatctgtgaa ggaagtccgt cctcgctcat
     2221 ggggagtggc ggcgtttcct atgtgtggag cgataattca accgccaatc ctgacacaac
     2281 ttacccgaac cttacgacaa cctatacagt taccggaacg gacaccaacg gctgttcgaa
     2341 tacggatacc gtaatggtga atgtgctctg ggctcccccg gtttctgcaa cggcttccgt
     2401 gcctacgatc tgctcttcgg gcggctctgt tcttaccgca tccggatcac aatcctatac
     2461 atggatgccc ggtagcatga ccggatcatc cattaccgtt accccttcct cgacgacgac
     2521 gtacacggtc accggaatga attcca
//
LOCUS       DASTSF010000354         3662 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_506330,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000354 DASTSF010000000
VERSION     DASTSF010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3662
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_506330"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..428)
                     /locus_tag="VFU15_10070"
     CDS             complement(<1..428)
                     /locus_tag="VFU15_10070"
                     /inference="COORDINATES: protein motif:HMM:NF018696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HEU4718172.1"
                     /translation="MPEIASPLSGKACRPVKTIGAGTIVDLYRKEYDIDVSAFFGNDP
                     LITIYECPDSGYRFYHPFTVFGDPEFYAQLENRSGYYPDWKWENEIALRFIPENSGLL
                     DIGCGNGSFIRQLSVKRKINATGIDTNSSAVREGKNLGSSL"
     gene            complement(428..1087)
                     /locus_tag="VFU15_10075"
     CDS             complement(428..1087)
                     /locus_tag="VFU15_10075"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TylF/MycF/NovP-related O-methyltransferase"
                     /protein_id="HEU4718173.1"
                     /translation="MNLLSLSSVPSRYRKIYKLVKDYTMIPEPVFYDNLELAEKYDTG
                     KGCIVECGVWRGGMSAAMGMLLGNEREIFLFDSFEGLPPAKELDGEKAISWQKDTSSP
                     FYFDNCKASEKEAETILDRAGIKNRKLVKGFFSETLPSFTPPSPISVLRLDGDWYDST
                     MDCLENLYKFVAPKGLILIDDYYLWDGCARAVHDFLSRHSLPDRIRQFNNGDLFYIIK
                     G"
     gene            complement(1084..2283)
                     /locus_tag="VFU15_10080"
     CDS             complement(1084..2283)
                     /locus_tag="VFU15_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU4718174.1"
                     /translation="MKPAIEIHNLSKIFRLRHDRRPYLSMRESLSGMFRFRKIETETF
                     YALNDVSFDVNPGDSIGIIGKNGAGKSTLLKVLSRITPPTSGRIKLRGRVASLLEVGT
                     GFHPELSGRENIFLNGSILGMKRNEILQNFDAIVDFAGVEKFIDTPLKHYSSGMQLRL
                     AFAVAAFLQPEILIIDEVLAVGDAEFQKKCMGKMEDISKSGRTILFVSHNLGAVNQLC
                     RSTVLLRDGKVSMTGKTSEVSRMYLDMDQKQARYSFDGTKENGMFFRSVHTENSRREE
                     KREFMHNESMHVVSVIDTAKYTNGMWLCIALLDKLKSRVFSAITPVAPFYSGKKETTI
                     TLTVPPDIIAPNHYSFLAAVYFPGGGHTDWLDDICPVFIADNGTEFASYNDYGNVILH
                     NCKWSSE"
     gene            complement(2286..3122)
                     /locus_tag="VFU15_10085"
     CDS             complement(2286..3122)
                     /locus_tag="VFU15_10085"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU4718175.1"
                     /translation="MEQLKTAPEETDILPPSRFSIGFAELWHYRELFYFFTWRDIKVR
                     YKQTFLGVLWALMQPLLMTPILVFSFGRNLQQFTPVPYPVFVFTGFVLWNIFSTGLSG
                     AGNGMVNNANMIKKIYFPRLIIPFSSVLVAMFDFLMTLPVIIGIFAWYHCWPGPEAIF
                     FFPAAIILTFMATVGAGSLFSALTVKYRDFRYVLQFLIQLLLFLSNVIYPLASVPNPW
                     VRYLLALNPMSGAITLMRGMYPNHVINWELFGISFCSGLVLFVTGLWYFRKTETYFAD
                     LA"
     gene            complement(3110..>3662)
                     /locus_tag="VFU15_10090"
     CDS             complement(3110..>3662)
                     /locus_tag="VFU15_10090"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HEU4718176.1"
                     /translation="ERTHGSLNFPFSEIRSLIEDHHSGKSYGWEYKMLVDLSIYHRHL
                     ETIFRYFPKERVHIFLFEELVRQPADVCGKLFRELGVDEDFRPDTSVQYNVTKEIHSH
                     KYARFVKRILRRENPLKKAVGVLLPGKTNYRIGEFLRRLNKKDIKPEMIDAESRKFLL
                     DYFRPHNDILAEMIGRDLSSWNS"
BASE COUNT          834 a          962 c         1018 g          848 t
ORIGIN      
        1 agagacgaac cgagattttt tccttcccgg accgcggagc tgttcgtgtc gatgcccgtt
       61 gcatttattt tccgcttcac cgaaagctgc cggatgaatg aaccgttccc gcaaccgatg
      121 tcgagcaggc cggagttttc cggaatgaaa cggagcgcaa tctcattttc ccatttccag
      181 tcgggataat agccgctcct gttctccagc tgcgcataaa attccgggtc gccgaaaaca
      241 gtgaagggat ggtagaaccg gtaaccggaa tcaggacatt cgtaaatcgt gatgagggga
      301 tcgttcccga aaaaagcgga aacatcgatg tcgtattctt tccggtaaag atcgacgatc
      361 gttccggccc cgatggtctt taccggccgg cacgcttttc cgctgagtgg tgacgcgatt
      421 tccggcatta gcctttgatg atgtaaaaaa gatccccgtt gttgaactgg cggatgcgat
      481 cgggaaggga atgccgcgaa agaaagtcgt ggaccgctct tgcgcacccg tcccaaaggt
      541 aataatcgtc aataagaatg agtcctttcg gcgccacgaa tttgtacagg ttttccaggc
      601 aatccatcgt cgagtcgtac cagtcgccgt cgagccggag aacgctgatc ggtgaaggtg
      661 gtgtgaacga cgggagcgtt tcgctgaaaa aacctttgac cagcttgcgg ttttttatgc
      721 ctgcacggtc gaggatcgtt tccgcttctt tttccgaagc tttgcagttg tcgaaataaa
      781 aaggagagga tgtgtctttc tgccagctga tcgctttttc accgtccagc tcttttgccg
      841 gtggtaatcc ttcgaaactg tcaaaaagaa aaatttctct ttcgttgccg agcagcattc
      901 ccattgccgc cgacatgccg cctctccaca caccgcattc cacgatgcac ccttttcccg
      961 tatcgtattt ttccgccagc tccaggttgt cgtagaaaac aggttccggg atcatcgtat
     1021 aatcctttac cagcttgtat attttccggt agcgtgatgg aacggatgac aggctgagca
     1081 ggttcattca gacgaccatt tacaattgtg aaggatgaca ttcccgtaat cgttgtacga
     1141 agcgaattcc gttccgttgt cggcaatgaa cacgggacag atgtcgtcga gccagtcggt
     1201 atggccaccg ccgggaaaat aaaccgcggc aaggaaggag taatggttcg gtgcgatgat
     1261 gtccggcgga acggtcagcg taatggtggt ttcttttttc ccgctgtaga aaggagcgac
     1321 gggtgtgatg gcggaaaaga cgcgcgattt cagcttgtcg agcagcgcta tgcagagcca
     1381 catgccgttc gtgtactttg ccgtatcgat aacggaaacc acgtgcatgg attcattgtg
     1441 catgaactcg cgtttttctt ccctgcggct attctccgtg tggacggaac ggaagaacat
     1501 cccgttctct ttcgtaccgt cgaatgaata acgcgcctgc ttctggtcca tgtcgaggta
     1561 catgcggctg acctccgatg tttttccggt catgctcact ttgccgtccc tgagcagcac
     1621 ggtggaacgg cagagctggt tgacggcgcc gaggttgtgg ctgacgaaga ggatggtgcg
     1681 cccgctcttg ctgatgtcct ccatcttgcc catgcatttt ttctggaatt cggcgtcacc
     1741 caccgccagc acttcatcga tgatcaggat ctccggctga aggaacgccg ccacggcgaa
     1801 tgcaagacgg agctgcatgc cgctgctgta atgtttcagc ggcgtgtcga tgaatttctc
     1861 cacgccggca aaatccacga tcgcgtcgaa gttctgcagg atctcgttcc gcttcatccc
     1921 gaggatggag ccgttgagaa aaatattttc ccgcccgctt agctccgggt gaaaaccggt
     1981 tcccacttcg aggaggctcg ccacgcggcc ccgcagcttt attctccctg atgtcggggg
     2041 cgtgatgcgc gagagcactt tcagcagcgt tgattttccc gcgccgttct tcccgatgat
     2101 gccgatggaa tcgccggggt tcacgtcaaa gctgacgtcg ttcagcgcat agaatgtttc
     2161 cgtctcgatc ttccggaaac ggaacatgcc cgagagcgat tcgcgcatgc tcaggtaggg
     2221 ccgccggtcg tgccggagcc ggaagatctt tgacaggtta tgtatctcta tggccggttt
     2281 catgatcagg cgaggtcggc gaaatacgtt tcagttttac ggaaatacca gagaccggtg
     2341 acaaagagca ccaggcccga acaaaagctg atcccgaaaa gctcccagtt gatcacgtgg
     2401 ttgggataca taccgcgcat gagcgtgatc gcaccgctca tcgggttgag cgcaagcagg
     2461 tagcgcaccc acgggttcgg cacggacgcc agcgggtaga tcacgttcga caggaacagc
     2521 agcagctgga tcaggaactg gagcacgtaa cggaaatcgc ggtatttcac cgtgagcgca
     2581 gaaaaaaggc ttcccgcacc tactgttgcc atgaaggtga ggatgatcgc ggcgggaaaa
     2641 aagaagatcg cttccggccc gggccagcaa tgataccacg cgaatatccc gatgatcacc
     2701 gggagcgtca tcaggaagtc gaacatcgcg acaagcacgg acgagaaggg aatgatgagc
     2761 cgcgggaaat agatcttctt gatcatgttc gcgttgttca ccatcccgtt gcctgcgccg
     2821 gaaagcccgg tggaaaaaat attccagagc acgaagccgg tgaaaacaaa aacagggtag
     2881 ggaacaggtg tgaactgctg gaggttccgc ccgaaggaaa aaacgaggat cggcgtcatc
     2941 agcagcggtt gcatgagcgc ccacagcaca ccgaggaacg tctgcttata acgcaccttg
     3001 atgtcacgcc aggtgaaaaa atagaacagc tcgcggtaat gccacagttc ggcaaaaccg
     3061 atgctgaacc gcgaaggcgg caggatgtcc gtttcttccg gtgctgtttt cagctgttcc
     3121 atgacgatag gtcgcgtccg atcatttcag caagaatatc attgtgcgga cgaaaatagt
     3181 ccagcaggaa tttacggctt tccgcatcga tcatttccgg tttgatgtct ttcttgttca
     3241 gccggcgcag gaattccccg atgcggtaat tggtcttccc gggcagcaaa acaccgacgg
     3301 ctttcttcag cgggttttcc ctgcggagaa tgcgcttgac gaaacgtgcg tatttgtgcg
     3361 aatggatttc cttcgtcacg ttgtactgca cgctcgtgtc gggcctgaaa tcctcatcca
     3421 cgccgagctc acggaacaat tttccgcaga cgtccgccgg ctgccgcacg agctcctcga
     3481 acaggaaaat gtgcacgcgc tccttcggga aataacggaa gatcgtttcc aggtggcggt
     3541 gataaatgct gaggtcaacg agcatcttgt attcccagcc gtacgatttc ccgctgtgat
     3601 gatcttcgat caggctgcgt atttccgaaa aaggaaagtt cagcgagccg tgcgtgcgct
     3661 cc
//
LOCUS       DASTSF010000355         4555 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1137470,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000355 DASTSF010000000
VERSION     DASTSF010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4555
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1137470"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="VFU15_10095"
     CDS             complement(<1..184)
                     /locus_tag="VFU15_10095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718177.1"
                     /translation="MYRPALLFLFLLFSCFAFAQNDTAKIPARTDSLSAAPVHPDTLA
                     VYPPPDTGIASWYGNKW"
     gene            309..1040
                     /locus_tag="VFU15_10100"
     CDS             309..1040
                     /locus_tag="VFU15_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013684959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HEU4718178.1"
                     /translation="MPEKVVIIPTYNEIENIEKMVRRVMSLPGDFHLLIVDDGSPDGT
                     ATKVKELMLEFPGRVFIEERKGKLGLGTAYIHGFRWALKNNYEYIFEMDCDFSHNPDD
                     LPRLYEACAKGGADVAVGSRYVKGGKVQNWPMNRLLMSYFASLYVRMVLWFGVKDTTA
                     GFKCYKRRVLEKIDLDRIKFVGYAFQIEMKYTAWKLGFKIAEVPITFIDRLEGVSKMS
                     TKIFKEAFWGVLQMRFRKIVPAKAV"
     gene            1130..1528
                     /locus_tag="VFU15_10105"
     CDS             1130..1528
                     /locus_tag="VFU15_10105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718179.1"
                     /translation="MKFLTATLFILLFSFALRAQDPWGGICCTPAPHSYFRSVHFHMP
                     GSAYLPSFPLTGKPHFRPDPGCDAYGQARPCDFSTFRQFTLTRSSFLDFTFPPTAYFC
                     RMENFTSDRYGIMFSVHAGGFSEEDEQAGW"
     gene            1596..2672
                     /locus_tag="VFU15_10110"
     CDS             1596..2672
                     /locus_tag="VFU15_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020402786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718180.1"
                     /translation="MRFLFLTPLLFAATCLLISGKTPTKNIDNPQEKHLKNLRQLTFG
                     GDNAEAYWSPDGKMLTFQSNYKKWGNSCDQIYLMKIADAKDSTYTPPRISNGEGRTTC
                     SYFMPDGKHILFATTFKGGKECPPTGDLHKDGKYLWPVFDSYDIFVADLKGNITQRLT
                     DSPGYDAEAVVSPKGDKILFTSTRGGDLDLWTMNIDGSNPVQITHDLGYDGGGFFSPD
                     GTKIVFRASRPTTDEEVKDYKDLLAQGLVAPTNMEIYTCNVDGTDQRQITNLGKANWA
                     PYFTPDGKKIIFSSNHQSAKGFDFQLFMISAEGGEAPEQITFESYFNAFPMFSPDGKH
                     IAFSSNRLNGGTHDTNVFVADWVE"
     gene            2902..4128
                     /locus_tag="VFU15_10115"
     CDS             2902..4128
                     /locus_tag="VFU15_10115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015029772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEU4718181.1"
                     /translation="MKIRYAFLLAFLFPLFLSAQDIDTGRLKKDISFLASDKLQGRGT
                     GSDGEKMAADYISGQFKKCGLQPKGTNGYFQPFTFKHKVDPKDSTSKEEVRHGKNVIG
                     YLDNGAKLTVVIGAHYDHLGLGYDHNSLDANPEGKIHNGADDNASGAAGVMELARYFT
                     SNKEKEPFNFLFICFSGEELGLIGSKKFCDSATIDLSTVDYMINMDMIGRMNDSTKTL
                     IIYGVGTAPDWVPMIDGIQTGMHIKKDSAGIGPSDQTSFYLKNIPVLHFFTGQHEDYH
                     KPSDDENKINYPGEKMVLQYIITIIDKTEKLDKLRFLQTKNPDVGRHKYSVTMGVMPD
                     YAFEGKGMRVDGVTDGKPAAIAGVKQGDVIIQLGDYPVGSTMDYMKALGNFKKGDTTK
                     VKVKRGEQELSLDVTF"
BASE COUNT         1179 a         1174 c         1165 g         1037 t
ORIGIN      
        1 tccatttgtt gccgtaccac gatgcgatgc cggtatcggg tggcgggtaa acggcaagtg
       61 tatcgggatg cacaggcgca gcagacagcg aatcggtccg ggcgggaatc ttcgccgtat
      121 cgttctgcgc aaaagcgaaa caggaaaaaa gaagaaacag gaaaagcagg gccgggcgat
      181 acatgctgaa atttaggatt tcctttcaag ccggggcgtt tccgctcgtc ggcaaacccg
      241 gctaaaataa gttaacgcat tgcaggataa aaacagcacg actatcttcg ttgattcaaa
      301 acgcgactgt gccggaaaaa gtagttatta tccccacgta caacgagatc gagaacatcg
      361 aaaagatggt gcgcagggtc atgtcgctcc ccggcgattt tcacctgctt atcgtcgatg
      421 acggttcgcc cgatggcacg gccacgaaag tgaaagagtt gatgctggaa tttcccggcc
      481 gtgttttcat cgaggaacgg aaaggaaagc tcgggctcgg caccgcatac atccacggtt
      541 tccgctgggc gctgaaaaac aattacgaat acattttcga aatggattgc gacttttcgc
      601 acaaccccga tgatcttccg cggctttacg aagcctgtgc gaaaggcgga gcggatgtgg
      661 cggtaggttc gcgctacgtg aaaggcggca aagtgcagaa ctggccgatg aaccgtttgc
      721 tgatgtcgta cttcgcttcg ctgtacgtgc gcatggtgct ctggttcggc gtgaaagata
      781 cgacggcagg cttcaaatgc tacaaacgcc gcgtgctgga aaagatcgat ctcgacagga
      841 tcaaattcgt cggctacgct ttccagatcg aaatgaaata caccgcgtgg aagctcggtt
      901 ttaaaatcgc ggaagttccc atcacgttca tcgaccgtct cgaaggtgtt tccaagatga
      961 gcacgaaaat tttcaaggaa gctttctggg gcgtgctgca gatgcgcttc aggaagatcg
     1021 ttccggcgaa agcggtctga ggtttttctt ttctgtttgc cgtcactgtc ctttatcgcc
     1081 gcctcgcgtg aaccggttgg ctattcggaa tttccgtaaa tttaattgta tgaaattcct
     1141 cacggcgaca ttgttcatcc tgctcttttc ctttgcactt cgcgcgcagg atccctgggg
     1201 aggaatttgt tgtaccccgg ctccgcacag ctatttccgt tcggtacatt ttcacatgcc
     1261 cggttccgct tatctcccgt ctttcccgtt aacgggtaaa ccgcattttc gtccggaccc
     1321 cgggtgcgac gcttatgggc aggcgcgtcc atgtgatttc agcacgttcc ggcaattcac
     1381 gctgacgcgc tcctcctttt tggatttcac ttttccgccg actgcgtatt tctgccgcat
     1441 ggagaatttc accagcgacc gttacgggat catgttcagt gtgcatgcag gcggattttc
     1501 agaggaagat gagcaggccg gatggtaaga attcctgaca ttgctcagac aaaatctcct
     1561 gcttctttcg gtatttttgc gctctaccga aaactatgcg cttcctcttt ctgacgcccc
     1621 tcttgtttgc tgcgacgtgc cttctcattt ccggcaaaac accaacgaaa aacatcgaca
     1681 atccccagga aaagcacctg aaaaatctcc gccagctgac gttcggcggc gataacgccg
     1741 aagcttactg gagtcccgac ggaaagatgc tcaccttcca gtcgaactac aaaaaatggg
     1801 gaaattcctg cgaccagatc tacctgatga agatcgccga cgcgaaagat tccacgtaca
     1861 cgccaccgcg catcagcaat ggggaaggac gcaccacgtg ctcgtacttc atgcccgacg
     1921 gtaagcacat cctgttcgcc acgacgttca agggtggaaa agaatgtccg ccaaccggtg
     1981 atcttcacaa ggacggaaaa tacctctggc cggttttcga ttcatacgac attttcgtgg
     2041 ccgacctcaa aggaaatatc acgcagcgtc tcaccgattc acccggctac gatgcggaag
     2101 ccgtcgtttc cccgaaaggc gataaaattc ttttcacgtc cacgcgcggg ggcgacctcg
     2161 atctctggac catgaacatc gacggcagca accccgtgca gatcacgcac gatctcggtt
     2221 acgacggcgg cggatttttt tcacccgacg ggacgaaaat tgttttccgc gcgtcgcgtc
     2281 cgactacaga cgaggaagtg aaagattaca aagacctgct tgcgcaggga cttgtcgcgc
     2341 ccacgaacat ggaaatttat acctgcaatg ttgacggaac cgatcagcgg cagatcacca
     2401 acctcggcaa agcgaactgg gcgccgtact tcacacctga cggaaagaaa attatttttt
     2461 catcgaacca ccagtcggca aaaggattcg atttccagct cttcatgatc agcgccgaag
     2521 gcggagaagc gcccgagcag atcacgttcg aaagctattt caacgcgttc cctatgttct
     2581 cgcccgacgg gaagcacatc gcgttttcct ccaaccgtct caacggcggc acgcatgaca
     2641 cgaatgtatt cgtggcggac tgggtggagt aagcggcgat ctgcgatttc agctgcggac
     2701 gctttcctgc agggctttca cggcctttcg gtggcgggcc gtcatgcgca gttcgcatgg
     2761 gacgggcagg aagatgcgat cgtttcaggt ttccgcgctg cggagaaaat ttcagcttcc
     2821 acctgatcct tcaaaaaaag cgattctgcc gaaggatttg cccgcccggg agttattttt
     2881 gtgtgaatca aaaaaagctc tatgaaaatc cgttacgcat ttttgctggc gttccttttt
     2941 cctctctttc tttctgcgca ggatatcgac accggtcgtt tgaagaaaga tatttccttc
     3001 ctcgcttccg ataagctgca gggccgcggc actggttccg acggcgaaaa aatggccgcc
     3061 gattatattt cagggcaatt caaaaaatgc ggacttcagc cgaaaggcac gaacggttat
     3121 ttccagccgt tcacttttaa gcacaaggtc gatccgaaag acagcacgtc gaaagaagaa
     3181 gtgcgtcacg ggaaaaacgt gatcggctat cttgacaatg gcgcgaagct gacggtcgtg
     3241 atcggcgcgc attacgatca cctcggtctc ggctacgatc acaattccct cgatgcgaat
     3301 cccgaaggta aaattcacaa cggcgccgat gacaacgctt ccggtgcggc cggtgtgatg
     3361 gagctcgcgc ggtactttac ttcgaacaag gaaaaagaac cgttcaattt tctcttcatc
     3421 tgcttttccg gcgaagagct cgggctcatc ggttcgaaaa aattctgcga ttccgcaacg
     3481 atagatctct cgacagtcga ttacatgatc aacatggaca tgatcgggcg catgaacgac
     3541 tccacgaaaa cgctgatcat ttacggtgtc ggcaccgcgc ccgactgggt tcccatgatc
     3601 gacgggatac agaccggcat gcacatcaaa aaagacagcg ccggcatcgg gccttccgac
     3661 cagacatcgt tctacctgaa aaatattcct gtgctgcact ttttcaccgg acagcatgaa
     3721 gattaccaca agccttccga cgacgagaac aagatcaatt atcccggcga gaaaatggtg
     3781 ctgcagtaca tcatcacgat cattgacaaa acggagaagc tggacaagct ccggttcctg
     3841 caaacgaaaa atcctgatgt cggacggcac aagtacagcg tgacgatggg cgtcatgccg
     3901 gattacgcgt tcgaaggaaa aggcatgcgc gtggacggcg ttaccgatgg aaaaccggcc
     3961 gcgatcgcag gcgtcaagca aggtgatgtg atcatccagc tcggcgatta ccctgtaggc
     4021 agtacgatgg attacatgaa ggcgctcggt aattttaaaa agggcgacac gacgaaagtg
     4081 aaagtgaagc ggggagagca ggaattgtcc ctcgacgtca cgttctgagg taacatttag
     4141 cagagcttaa gttcttagcg tcgccggtga aaaagacttt gtgcgcattg tggcaaaaaa
     4201 aacaggcatg accaaaacac tcatccgtaa cgcaacgatt taaataaaca actttgtgtc
     4261 gctggtgaaa aagagtttgt gcgctttgtg gtaaaaaaaa caggcatgaa caaaacactc
     4321 atccgtaacg caacgattta aataaacaat tttgtgtcgc cggtgaaaaa gagtttgtgc
     4381 gcttcgtggt aaaaaaacag gcatgaacaa aacgctcatc cgtaacgcaa cgatttaaat
     4441 aaagaacttt gtaacctcag tgaaaaagac tttgtgcgct tcgtggtaaa aaaaaacagg
     4501 catgaacaaa acactcattc gtaacgcaac gatttaaata aacaactttg tgtgc
//
LOCUS       DASTSF010000356         6129 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_486949,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000356 DASTSF010000000
VERSION     DASTSF010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 6129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6129
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_486949"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1317
                     /locus_tag="VFU15_10120"
     CDS             <1..1317
                     /locus_tag="VFU15_10120"
                     /inference="COORDINATES: protein motif:HMM:NF012936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase C-terminal domain-containing
                     protein"
                     /protein_id="HEU4718182.1"
                     /translation="GSGNGMSGTMRELQNSHFIQFTRPGNHTSSSYYQLPSRTITIDY
                     ETAKKELAQKLEEAVSSRLIADVPLGSFLSGGLDSSIITALASRHKKDLETFSIGFAD
                     EPFFDETEYAKLVSKHLGTQHHVFSLTNNDLLESLHEFLENCDEPFADSSALALNVLA
                     RETRKHVTVALSGDGADELFAGYNKHEAELRIRMKSGLTRIAKHGSFLWDILPASRNS
                     SLGNRMRQLRKFAEGIKMTAAERYWRWAGFTKEGDLGKLIQNPQITSSIPKRFCRDDI
                     SARIHDDGDFNEILREDFKMVLEGDMLVKVDRMSMLHGLEVRPPFLDHEVVDFVMQLP
                     AEYKIQPGNRKRILRDAFAGLLPKEIYTRKKQGFEVPLLKWFRNELSGMIEELLNEKF
                     LEEQNIFNAEEVTRIRKQLHSNNPGDSAARTWGLIVFQKWWLNYFG"
     gene            1453..2646
                     /locus_tag="VFU15_10125"
     CDS             1453..2646
                     /locus_tag="VFU15_10125"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU4718183.1"
                     /translation="MPRILRIINRFNLGGPTYNVAYLTKFLSPEFETLLVGGSKDETE
                     DSSEFILENLGLKPVIIPEMKREISIRQDRIAYKKIKALIREFKPDIVHTHASKAGAL
                     GRRAAYACHVPVIVHTFHGHVFHSYFGKLKTALYLNIERRLARKTDAIIAISEKQKQE
                     LSETFRIAPAEKFHVIPLGFDLERFRTGTEAKRKAFREKYQVADDEIAIGIIGRLVPI
                     KNHRLFLDAFLKAKGMTSKKIKGFIIGDGELKTELMEYCKTAGLSFTSRTGLHPSADI
                     FFTSWIKDADVAIAGLDIVALSSLNEGTPVSLIEAQASGRPVVSTRVGGVENIVLPDR
                     SALLSDPGDAEEFARHLLRLVEDEALRGSFGNCGSEQIGERFSYTRLTSEMGALYRRL
                     LQSKA"
     gene            2697..3497
                     /locus_tag="VFU15_10130"
     CDS             2697..3497
                     /locus_tag="VFU15_10130"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HEU4718184.1"
                     /translation="MKKTVFRFAGFIVVVAFLIFSDSCNVLNPNIMLHAGRKYKYDTL
                     ANDSTVAKEFTLGPNDIIEFRLFANDGFKMIDIISSGNTGASNNTLMRTGFEYTLDDS
                     GTVRLPVVGDQNLTGLTVRQAEDSLQKKYSEYYVDPFVIIKIINKRVIIYNGEPGQAK
                     VVGLSNNNTTIIEALALAGGISGDGKAYNIKLIRHTTDPKKPIKVYKINLSKIDGIKD
                     GSTIVQSGDIIYVEPRKQIAGKALREVTPVLSLTTSLLTFYYLITKLL"
     gene            3516..5963
                     /locus_tag="VFU15_10135"
     CDS             3516..5963
                     /locus_tag="VFU15_10135"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HEU4718185.1"
                     /translation="MLQAQITDRNETLERYKERLTNFSGEFELGLFLFIARKSLYWIA
                     FFFLSAFFFSWLYLRYTQTEYESTALIQINTDNNAKVLNVSNIYEVEDQNELPGAVEM
                     IRSKVFLRRVLSKLPLQVSYFAEGTFKTNEHYKSSPYTVDINVKSESVLGTRFDISFS
                     KNLEGGEISCMSNGVKVTRTFIVGQWVEFPDISLLVTTNREKLNQMLSGDEHANNYYF
                     TVNNLEDLADAYSQRLTVALANEAAKTIFISFTDNNPRKAYDIVNTITSEYIQYDVER
                     RSESAKKALDFIDEQLRNVSDSLRSSEDSIKSFRQRKHIEDNEALNTFNMTRYGSIED
                     QYTKIELEEQVMNEVEQKMTQPDVDVKSLLSLVSGTEHEQALAPAMNNLYTLISERDQ
                     ALFEVKPKSDIIKSYDYRIGVQKNIIIESVKSILAQLAIRKKAIGEKLDQYGAKLPGD
                     QSEEVEFTSLQRLYDINQKFYSLLIEKKTEYAISEAGFTPMHVVLEKPRIPAGPKSPS
                     RRNTFITATLLAFLAGLILVVIRYLMHDKISSLNEIMKHTQASISALGIIPKYKENIP
                     VSQLLVDRNPKSLIAEAFRSLRTNLQFISNEAGPKVMAVTSTISGEGKTFVAINLAGI
                     ISYSGKKVIILDLDMRKPKIHIGFNVSNEKGMSTLLIGRDKLEDCIRRSSQENLHFIT
                     AGPIPPNPSELIIGDAMMEVIEELKKTYELIIIDNPPVGLVTDGLHMIQKADYPIYIF
                     RADFSRRSFIQIVDRLYNELGIKKLSVVLNGVDVDRRGYGYNYGYGYGYGYGYGQGYY
                     DEGKQRKKRFSFFRRSS"
     gene            5973..>6129
                     /locus_tag="VFU15_10140"
     CDS             5973..>6129
                     /locus_tag="VFU15_10140"
                     /inference="COORDINATES: protein motif:HMM:NF013103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HEU4718186.1"
                     /translation="MKIETTPIDGLLVIRPDVFADSRGYFFESFNKKKFSDAGIACEF
                     VQDNQSLS"
BASE COUNT         1679 a         1616 c         1522 g         1312 t
ORIGIN      
        1 ggatcgggca acggcatgag cggaacaatg cgtgagcttc aaaattctca cttcatccaa
       61 ttcacaagac ccggtaatca caccagctcc tcttattacc agctgccttc acgtaccatc
      121 acgattgatt atgaaacggc aaaaaaggaa ttagcgcaaa aactggagga ggctgtcagt
      181 tctcgattga ttgctgatgt tccgctcggt tctttcttaa gcggcgggct cgactcttcc
      241 atcataaccg cattggcatc acgacataaa aaagatcttg aaacattttc catcgggttt
      301 gcagatgagc cgttcttcga cgaaacggaa tatgcaaaac ttgtttcgaa acatctcgga
      361 acgcagcacc atgttttctc attgaccaat aacgacctgc ttgaatcgct gcatgaattt
      421 cttgagaatt gcgacgagcc gtttgccgat tcttctgcac ttgcgcttaa tgttctcgcg
      481 agagaaacac gaaaacacgt aaccgttgca ctttccggtg atggagcgga tgaacttttt
      541 gcaggataca acaagcatga agcggaattg agaataagaa tgaaaagcgg tttgacacga
      601 attgcaaaac acggatcatt tctttgggat attcttcctg catcaagaaa ttcttcgctt
      661 ggaaaccgta tgcgccaatt aaggaaattt gcggaaggca ttaaaatgac cgctgcagaa
      721 cgttactggc gatgggccgg atttacaaaa gaaggagatc tcggtaaatt gattcaaaac
      781 ccacagataa cctcatccat ccccaaaaga ttctgccggg acgatatttc ggcacgcatc
      841 catgatgacg gtgatttcaa cgaaattctc agggaagatt tcaaaatggt cctcgaaggc
      901 gacatgctcg tgaaagtcga ccgcatgagc atgctgcacg ggctcgaagt acgtccgcct
      961 tttctcgatc acgaagtggt ggatttcgtg atgcagcttc cggcggaata caaaatccag
     1021 ccggggaaca ggaaacggat actcagggat gcgttcgccg gtcttcttcc gaaagaaatt
     1081 tacacgagaa agaaacaggg attcgaagtt ccgctgctga aatggttccg gaatgaactg
     1141 agcggaatga ttgaagaact gctcaatgaa aaattcctgg aggaacagaa cattttcaac
     1201 gcggaagaag tgacgcgcat ccggaaacag ctgcactcga acaatcccgg cgacagcgca
     1261 gcccgtacct ggggactcat cgtgttccag aaatggtggc tgaactattt cgggtaaatt
     1321 tcaaagccca aaacggaaat aaccaagttc aaagttgaaa aattgcagcg tttgaaagtt
     1381 cccggatcca aatgacaaac ccgaaatccg aaaccggaaa cccgaaaccc gaaactccaa
     1441 actgaaaacc gaatgcccag gattctccgc atcatcaatc gtttcaatct cggcggaccg
     1501 acgtataacg tcgcgtacct cacgaaattc ctttcgccgg aattcgagac gctgctcgtg
     1561 ggcggatcga aagacgaaac ggaggacagc tcggaattca tcctcgaaaa cctcgggctg
     1621 aaaccggtga tcattcccga aatgaaacgc gagatcagca tccgccagga ccgcatcgcg
     1681 tacaaaaaaa taaaagcgct catccgggaa ttcaaacccg acatcgtgca cacgcacgcc
     1741 tcgaaagcag gcgcgctcgg gcgccgcgca gcgtacgcat gccatgttcc cgtgatcgtc
     1801 cacacgtttc acggtcatgt ttttcactct tatttcggga agttgaaaac ggcgctctac
     1861 ctgaacattg aacgcaggct cgcccggaaa acggacgcca ttatcgccat cagcgaaaaa
     1921 cagaaacagg aactttccga aacattccgc attgcgcccg cggaaaaatt ccacgtcatc
     1981 ccgcttggct ttgaccttga acgtttccgg acgggaacgg aagcgaaacg gaaagcgttc
     2041 cgtgaaaaat accaggtcgc cgatgacgag atcgcgatcg gaattatcgg ccggcttgtt
     2101 cccataaaaa atcaccgcct gttcctcgat gcgttcctga aagcgaaggg aatgacctca
     2161 aaaaagatca aaggattcat catcggcgac ggggaactga agacggaact gatggaatac
     2221 tgcaaaacgg ccgggctttc cttcacgtca cgcacgggat tgcatccttc cgccgatatt
     2281 tttttcacgt cgtggatcaa ggacgccgat gtggccattg ccggtctcga tatcgtcgca
     2341 ttgtcttcgc tcaatgaagg aaccccggtg agcctcatcg aagcgcaggc atccggccgg
     2401 ccggtcgtta gcacgagggt gggcggcgta gagaatatcg tgctgcctga ccgctccgct
     2461 cttctcagcg atccgggcga tgcggaagag tttgcgaggc atcttctccg cctggtggaa
     2521 gacgaagcgt tgcgcggcag cttcgggaac tgcggcagcg aacagatcgg ggaacgtttc
     2581 agctataccc ggttgacctc ggaaatgggt gcactttacc gcaggctgtt gcagtcaaag
     2641 gcctgaaacc gcgttttttg atacctttac gggctgattc ccaaccgaaa atcaatatga
     2701 agaaaacggt tttccgcttt gctggattta tcgtggtggt ggctttcctg attttctcgg
     2761 acagctgcaa cgttttgaac ccgaatatca tgctccacgc cggccggaag tacaaatacg
     2821 atacgctggc caacgatagc acggttgcca aagaatttac cctcgggccg aatgacatca
     2881 tcgaattcag gctcttcgcc aacgatggtt ttaaaatgat cgatattatt tccagcggga
     2941 acaccggggc atcgaacaat acgcttatgc gtacgggttt cgaatatacg ctcgatgaca
     3001 gcggcacggt ccgtcttccc gtcgtcggtg accagaacct taccggcctt accgtccggc
     3061 aggcggaaga ttcgctgcag aaaaaatatt ccgaatatta tgtcgatcct tttgtcatca
     3121 tcaagatcat caataagcgg gtgatcatct ataatggcga acccgggcag gcgaaggtgg
     3181 tcggcctcag caacaacaat acaacgatca ttgaagcgct cgcgcttgcc ggcggaattt
     3241 ccggcgacgg caaagcctac aacataaagc tgatcaggca cacaacggat ccgaaaaaac
     3301 cgatcaaagt gtacaagatc aacctctcga agatcgacgg cataaaggac ggaagcacca
     3361 tcgtgcagtc cggagatatc atttacgtgg aaccgagaaa gcagatcgcc ggcaaagccc
     3421 tcagggaagt aacgcctgtg ctttcgctga cgacttcgct gctcacgttc tactatctca
     3481 ttaccaaact cctctgaccc ggggactgaa cgactatgct tcaggcgcag attacggacc
     3541 ggaatgaaac gctcgaacgt tacaaagagc gcctgaccaa tttcagcggg gaattcgagc
     3601 tcggactttt cctttttatt gcgcgtaaat ccctttactg gatcgctttc ttttttcttt
     3661 ccgccttctt tttttcgtgg ctttacctcc gctacaccca gaccgagtat gagtcgacgg
     3721 cattgataca gatcaatacc gacaacaacg ccaaggtgct gaacgtctcg aatatttacg
     3781 aggtggaaga tcagaacgag ctgcccggcg cggtggaaat gatccgttcc aaagtgtttc
     3841 tccggcgcgt actgtcgaag cttcccctgc aggtgagcta tttcgcggaa ggaacgttca
     3901 agaccaacga gcattacaaa tcgtcgccgt atacggtaga catcaacgtc aagtcggaaa
     3961 gcgtcctggg cacacgcttc gatatttcgt tcagcaaaaa cctggaaggc ggcgagatca
     4021 gctgcatgag caacggggta aaagtcacac gcacattcat tgtcgggcaa tgggtggaat
     4081 ttcccgacat ctcgctgctg gtgacaacga accgggaaaa gctcaaccag atgctgagcg
     4141 gtgacgagca cgcgaacaat tattatttca cggtgaacaa cctcgaggat cttgccgatg
     4201 cctattccca gcggctgacc gttgcgctgg cgaacgaagc ggcaaaaacc atcttcatct
     4261 cgttcacgga taacaatccc cgcaaagctt acgacatcgt caatactatc acatccgaat
     4321 acatccagta cgacgtcgaa cgcaggagcg aaagcgccaa gaaagcgctt gattttatcg
     4381 atgaacagct gcgcaatgtt tccgactctc tccgctcttc cgaagattcg atcaaaagct
     4441 tccggcagcg gaaacacatc gaggataacg aagcgctgaa cacattcaac atgacacgtt
     4501 acggctcgat cgaagatcag tacacgaaga tcgagctcga agagcaggtg atgaacgagg
     4561 tggaacagaa aatgacgcag ccggacgtgg acgtaaagtc gctgctgtcg ctggtttccg
     4621 gcacagagca tgaacaggcg ctcgccccgg cgatgaacaa cctgtatacg ctcatttcag
     4681 aaagggacca ggcgttgttc gaggtaaagc cgaaaagcga catcatcaaa tcgtatgatt
     4741 atcgcatcgg ggtgcaaaag aacatcatca ttgaaagcgt caaatctatt ctcgcgcagc
     4801 tcgccatccg caaaaaagcg atcggggaaa agctcgacca gtacggcgcg aaacttccgg
     4861 gcgaccagag cgaagaggtg gaattcacca gcctgcagcg gctctacgac atcaaccaga
     4921 aattctacag cctgctgatc gagaaaaaaa cggaatatgc catctcggaa gccggcttca
     4981 ccccgatgca cgtggtgctc gaaaaaccgc gtatcccggc cggaccgaaa agccccagcc
     5041 ggaggaacac gttcatcacc gccacgctcc tcgcctttct cgccggcctc atcctcgtcg
     5101 tgatccgtta cctgatgcac gacaagatca gctcgctgaa cgagatcatg aagcacacgc
     5161 aggccagcat cagcgcactc ggcattattc cgaaatacaa ggaaaacata ccggtttccc
     5221 agctgcttgt cgaccgcaac ccgaagtcgc ttatcgccga agcgttccgt tccctgcgca
     5281 ccaacctcca gttcatttcc aatgaagcgg gtcccaaggt aatggccgtg acgtccacca
     5341 tctcgggtga aggaaaaaca ttcgttgcca tcaacctcgc cggcattatt tcctactcgg
     5401 gaaaaaaggt gatcatcctt gacctcgaca tgcgcaagcc gaagatccat atcggcttca
     5461 atgtctcgaa tgaaaaagga atgagcacgc tgctcatcgg ccgcgacaag ctggaagact
     5521 gcatccgcag gagctcccag gagaacctgc attttatcac ggccggaccc atccccccga
     5581 atccgtcgga gctgatcatc ggtgatgcga tgatggaagt tatcgaggag ctgaaaaaaa
     5641 catacgagct catcatcatc gacaacccgc ctgttggtct tgttacagac gggctccaca
     5701 tgatccagaa agcggattat cccatctata ttttccgtgc cgatttttca cggcggagct
     5761 ttatccagat cgtcgacagg ctgtacaatg agctgggtat taaaaaactt tccgtggtgc
     5821 tcaatggagt cgacgtagac cgcaggggct acggatacaa ctacgggtac ggatacgggt
     5881 acgggtatgg ctacggacaa ggatattacg atgaaggaaa gcagcgcaaa aaacgattct
     5941 ctttctttcg ccggagcagc taatttattg ttatgaaaat tgaaacaacg cccatcgacg
     6001 ggctgctggt gatcaggccg gatgtttttg cagactcccg cgggtatttc ttcgagtcct
     6061 tcaacaaaaa aaaattctcc gacgcaggca tcgcgtgtga attcgtgcag gacaaccagt
     6121 cgctgtcgg
//
LOCUS       DASTSF010000357         7271 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_454445,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000357 DASTSF010000000
VERSION     DASTSF010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 7271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7271
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_454445"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1356)
                     /locus_tag="VFU15_10145"
     CDS             complement(<1..1356)
                     /locus_tag="VFU15_10145"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEU4718187.1"
                     /translation="MKRIYTLLSLLALGPALFAINGSPVPVDKNAVNVASLKPDDYMH
                     NTLILKVLPEYRSLCSVERINIPAVNDFFLTMGEDQCGKKYPRHEAPKEKFNKYGMPM
                     IDLSLIYEIHYTGAMPMDMAIAKLMNLGFFEYVEPHIIPKISFVPNDPQATQAQSYYL
                     YKIAAAGSGTTGWDIETGNSNVVIGIVDTGTEYTHSDLSNQIKISSTEISGNGIDDDG
                     DGYIDNYRGWDVAMNDNDATWEGNAHGVAVCGDACAQTNNNNKVAGPGYNCKILPVKI
                     ADASGTLTAAYDGIVYAADHGCQVINCSWGGPSAGSYGQNIIDYAMNNKDALVVVAAA
                     NDGSDSAVYPAAYDNSMSVAATNSSDGKAYFSNYNYTVDVCSPGQGILNTWINNGTIS
                     NDGTSMASPVCAGVCAIVRAHYPSYNCYQTKQRVKTTADNIYSVSGNSAYQDKLGTGR
                     IN"
     gene            complement(1495..1947)
                     /locus_tag="VFU15_10150"
     CDS             complement(1495..1947)
                     /locus_tag="VFU15_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014680667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HEU4718188.1"
                     /translation="MELPVLNFPSYDFRLREEGGRAEIFDEWRKIWVRLTPEEWVRQH
                     AARWLKEEKKYPAGLTSLERTIRVNGMTKRCDVVAFGKSGEPVLIVECKAPDVRITQD
                     VFDQAARYNLSLGADLFFLTNGLNHYCCRADHEGKKYHFLKELPPYQY"
     gene            2019..2837
                     /locus_tag="VFU15_10155"
     CDS             2019..2837
                     /locus_tag="VFU15_10155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718189.1"
                     /translation="MTLEKLLCPQCGGNDLEPFGTDAYKCRFCGAVLKEEHEEKKEPE
                     THYVAPPSTDFSNRRTSYADDDDSDDEYNNKAVLKFVGIIVLAVAGIIAVFIGRSGNP
                     VPDPSPNITIPEMPLPSPDSFINALKKETEEINKKYETYYTAQGITADTAALLVSGKF
                     AGNETVPPGKKCVIRISNPSGFAVTNGKVSVQWSLVVHDENGNVTFEKDDYNSAASAE
                     GEDAKRYAAKADIPFAVSKAYGFAGPGYYFIDYKLLDHTNGNSFSGHIPFDVAK"
     gene            2897..3667
                     /locus_tag="VFU15_10160"
     CDS             2897..3667
                     /locus_tag="VFU15_10160"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HEU4718190.1"
                     /translation="MKTKEEIVSNWLPRYTGTPLKDWGEYILLTNFNNYVHMFAEQNK
                     VKVRGEDRPMPNAHANGISIINFGMGSATAATVMDLLSAIHPKAALFLGKCGGLKKKN
                     HIGDLILPIAAIRGEGTSNDYFPPEVPALPAFSLQKAISTTIRNHRRDYWTGTVYTTN
                     RRVWEHDNDFKEYLRATRAMAIDMETATIFTVGFHNEIPTGALLLVSDQPMIPDGVKT
                     EASDKKVTESFVNHHLQIGIDSLMELINNGLTVKHLRF"
     gene            complement(3671..4189)
                     /locus_tag="VFU15_10165"
     CDS             complement(3671..4189)
                     /locus_tag="VFU15_10165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718191.1"
                     /translation="MKQLFLCLLFFTAIRMNAQGDTLHAQCRIFMIRTQQVPQKNDSG
                     KADTFREKPYAVCRLSDSVFVIGNDTFDRVPPAKPLPVYFTMRDSAFLLMSVSRKTVP
                     FTIAAVSVDVMVNGVFVENMLDGDSVKPATKQLIDGVAPGGTIFLDAFRITDPSGKTV
                     KVQHDPLALKKR"
     gene            4358..5356
                     /gene="holA"
                     /locus_tag="VFU15_10170"
     CDS             4358..5356
                     /gene="holA"
                     /locus_tag="VFU15_10170"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014680666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEU4718192.1"
                     /translation="MEFNAILSDLKKKNYKPVYFLTGEEPYFIDVISDYVEKNVLDEN
                     EKEFNQTVLYGRDTDVQTIIAEAKRYPMMSERQVLIVKEAQNVRNIEDLEAYVEKPLD
                     TTLLLVCYKYKTLDKRKSFGKTVAKHGVLFESEKLRDYKIPEWVSTYLKEKNYSIGPK
                     ASQLLAEFLGSDLSKISNELDKLMINLPPGTEITAQHIQDNIGISKDYNTFELSDALS
                     KRDVVRANRIVNYFASNSKDHPMPVTIAALYNYFVKVLRYHGLENKAKDSVARALGVN
                     PFFVNDYAAAARNYPVGKLKAIFSFLREYDLRSKGVDNASAEDGDLMKEMVWKILH"
     gene            complement(5372..5761)
                     /locus_tag="VFU15_10175"
     CDS             complement(5372..5761)
                     /locus_tag="VFU15_10175"
                     /inference="COORDINATES: protein motif:HMM:NF020903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1987 domain-containing protein"
                     /protein_id="HEU4718193.1"
                     /translation="MRIEATRHTPLVEVDEKTSAIRIIGVSIPENAYVFFRPLEDYIE
                     ALDPENVTGVSFTFRLEYMNTLSSKAFLDYMRVVKDQKRIPLSVTWEYYSDDEEMKDH
                     GETFSEIVDIPFSYSSVNPKDRIPGRR"
     gene            5857..6261
                     /locus_tag="VFU15_10180"
     CDS             5857..6261
                     /locus_tag="VFU15_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013666797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HEU4718194.1"
                     /translation="MNYWLVKSEPAKYSWEKFLADGKTFWDGVRNYAARNHLRAMKKG
                     DLVFFYHSNEGLEIVGIAKVVKEAYGDPTTNEAAWVVVDLAPFKTLKKPVTLAEMKKE
                     KVLKNIPLIRIPRLSVMPVSKEEYERIMEMAS"
     gene            6381..>7271
                     /locus_tag="VFU15_10185"
     CDS             6381..>7271
                     /locus_tag="VFU15_10185"
                     /inference="COORDINATES: protein motif:HMM:NF025092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU4718195.1"
                     /translation="MRKVITRALLALLILPFTAAAQTATIRGTVSSSSNGEAVLFALV
                     YLEGTPYGVQTDLNGLYSLTKIPPGTYTLVVHESSFNPYKMTITVKAGDIITQNVSLE
                     PRVMKVVVISAHHQDVQNNPDVGKTSMDPKDINRVVAIGGVPDVAQSVQVLPGVVSSG
                     DQGGQLYIRGGTPVQNKVLLDGMVIYNPFHSIGLFSVFDTDIIKNLDVYTGGFNADYG
                     GRISSVMDITTRDGNKKHFAGKATVSPFGAKLLLEGPIRRMDDDPKSGYGAITYVVSA
                     KNSYLAQTSKLLYSYVDPNGI"
BASE COUNT         1823 a         1988 c         1825 g         1635 t
ORIGIN      
        1 gttgatgcgg ccggttccga gcttgtcctg gtacgctgaa ttaccggaaa cgctgtaaat
       61 gttgtcggcg gttgttttta cacgctgctt cgtctggtaa cagttgtacg aaggataatg
      121 cgcacgcacg atcgcacaga cacccgcgca aacaggcgaa gccatcgacg ttccgtcatt
      181 ggaaatggta ccgttgttga tccaggtatt caggattccc tgcccggggg agcaaacgtc
      241 taccgtgtaa ttgtaattcg agaaatacgc ttttccgtcg gagctgttcg ttgcagccac
      301 gctcattgaa ttgtcgtacg cagccgggta aacagcggaa tcggaaccgt cgttcgcagc
      361 ggcaaccacc acgagcgcgt ctttgttatt catcgcataa tcgatgatgt tctgtccgta
      421 agaaccggcg ctgggtccgc cccaggagca gttgatgacc tggcaaccgt ggtcggcggc
      481 ataaacgatt ccgtcgtaag cggcagtaag tgttccggaa gcatcggcga ttttcacggg
      541 aaggatttta cagttgtatc ccggtccggc cactttgttg ttattgtttg tctgtgcgca
      601 tgcatcaccg caaacagcga caccatgggc atttccctcc caggtggcat cgttgtcatt
      661 catcgcaacg tcccatccgc ggtaattgtc gatatatccg tctccgtcgt cgtcaatacc
      721 gttgccggag atctcagtag aagaaatctt gatctggttg gaaagatccg aatgcgtgta
      781 ttccgtcccg gtatcgacga tgccgatgac cacgttcgaa tttcccgttt caatatccca
      841 accggttgtg ccggaacctg ccgcagcgat tttgtagaga taatacgact gtgcctgcgt
      901 tgcctgcggg tcattcggaa cgaatgaaat tttcggaatg atgtgcggct ccacgtattc
      961 gaagaagccg aggttcatca gttttgcgat cgccatgtcc atcggcatcg caccggtgta
     1021 atggatttca tagatcagcg aaagatcaat catcggcatg ccgtacttgt tgaacttttc
     1081 tttcggcgct tcgtggcggg gatatttttt tccgcactgg tcttctccca ttgtcaggaa
     1141 aaaatcattg acggccggaa tgttgatccg ttccaccgag cagagcgaac ggtattccgg
     1201 aagaactttc agaatcaatg tattgtgcat gtaatcgtcc ggtttcagcg aagcgacgtt
     1261 cacagcattc ttgtcgacag gaacggggct accgttaatg gcgaagagcg cagggcccag
     1321 ggcgagcagc gataacagag tatagatcct tttcatggat tggttggtgt gagtataacg
     1381 tgaagcacga atgtagatat aaagcgctgt gaatcaaaac gtattaaaaa agaaaaatca
     1441 gaccgtctta cgcgcccggg acccgtttcc ggccgggaaa accggggagt aggatcagta
     1501 ttgatacggg ggaagctctt tcagaaaatg gtattttttc ccttcatgat ccgcgcggca
     1561 gcagtaatga ttcaacccat tggtgagaaa aaaaagatcc gcaccgagcg aaagattgta
     1621 ccgcgccgcc tggtcgaaaa catcctgcgt gatgcgcaca tccggcgctt tgcattcgac
     1681 gatgagaacg ggttccccgc ttttgccgaa agccaccacg tcgcaccttt tcgtcatccc
     1741 gttcactcgt atcgtccttt cgagcgaggt gagaccggca ggatattttt tttcttcctt
     1801 cagccaccgg gccgcatgct gcctcaccca ttcttccggc gtaagacgga cccaaatttt
     1861 tctccactca tcaaaaattt cagcgcggcc cccttcttcc cggagacgga aatcatacga
     1921 aggaaaattc aggacgggca gctccacgcg atgacttttc ccaaaattac gccgaaatga
     1981 ccgtgccgga aattctatct ttgatcaacc tcattcgtat gacactcgaa aagctgctgt
     2041 gcccgcaatg cgggggaaac gacctggaac cattcggcac ggacgcctat aaatgcaggt
     2101 tctgcggcgc cgtgctgaag gaagagcacg aagaaaagaa agagcctgaa acgcattacg
     2161 tcgcgccgcc ttccaccgat ttcagtaacc gcagaacgag ctatgcggat gacgacgaca
     2221 gcgatgacga gtataacaac aaagccgtat taaagtttgt gggaatcatc gtgctggctg
     2281 tcgccgggat catcgcggtg ttcatcgggc gttccggcaa tcccgttccc gacccttcgc
     2341 cgaacatcac catcccggaa atgcccctgc cttcccccga ttcgtttatc aatgcgctga
     2401 aaaaagaaac cgaggagatc aataaaaagt acgaaacgta ttataccgcc caagggatca
     2461 ctgcagatac cgccgctctt cttgtttccg ggaaattcgc cggcaatgag accgtgccgc
     2521 ccgggaaaaa atgtgtgatc cgcatttcaa atccttccgg cttcgccgtg acgaacggaa
     2581 aagtcagcgt gcagtggagc ctcgtcgtac acgacgaaaa cggcaacgtg acttttgaaa
     2641 aagacgatta caacagcgcc gcgtctgccg aaggcgagga tgcgaaacgt tacgccgcaa
     2701 aagcggatat tccttttgcc gtttcaaaag catacggatt cgccggacca ggttattatt
     2761 tcatcgatta caaattgctg gaccacacga acggaaattc tttttccggc cacattccct
     2821 tcgacgtggc gaaatgaatt aaccttccgt ttgcttttcc tgcttgctga aaaactacct
     2881 tcgcagggaa atcaccatga aaacgaaaga agaaattgtt tccaactggc ttccgcgcta
     2941 tacgggaact ccgctcaaag actggggaga atacattctc ctgacgaatt tcaacaatta
     3001 cgtgcacatg ttcgccgaac agaacaaggt gaaagtgcgc ggcgaagacc gccccatgcc
     3061 caacgcgcat gccaacggga tctccatcat caacttcggc atgggaagcg ccacggcggc
     3121 aaccgtgatg gatctcctca gcgccattca cccgaaagcg gcgcttttcc tcggcaaatg
     3181 cggcggactc aaaaagaaaa atcacatcgg cgatctcatt cttcccattg ccgccatacg
     3241 cggcgaagga acgagcaacg attatttccc gcctgaagtt cccgcacttc ccgcgttcag
     3301 cctgcagaaa gccatttcca ccacgatacg gaatcaccgg cgcgattact ggacaggcac
     3361 cgtttacacg acgaaccgcc gcgtgtggga gcacgacaac gatttcaagg aatacctccg
     3421 cgccacgcgc gccatggcta tcgacatgga aacggcgacg atcttcaccg tcggctttca
     3481 caatgaaatt cccaccggcg cgctgctgct tgtctccgac cagcccatga tccccgacgg
     3541 tgtgaaaacg gaagccagcg acaaaaaagt gacggaaagc ttcgtcaacc atcacctgca
     3601 gatcggcatc gattcgctca tggagctcat caacaacggg cttaccgtga agcacctccg
     3661 cttctgatct tcatcttttt ttcagcgcca gcgggtcgtg ctgcaccttc actgtttttc
     3721 ccgaaggatc cgtgatccgg aaggcgtcga ggaaaattgt tccgccaggc gcaactccgt
     3781 cgatcagctg cttcgtcgcc ggtttcactg aatcgccgtc cagcatgttt tcaacaaaca
     3841 caccgttcac cattacatcc acgctgactg ctgcgatcgt aaacggtact gttttccgtg
     3901 aaacgctcat gagaaggaac gccgaatccc gcatcgtgaa ataaacgggc agcggctttg
     3961 ccggcggaac ccggtcgaac gtatcgttcc cgatcacaaa aacgctgtcg gaaagccggc
     4021 ataccgcgta cggtttctcc cggaaagtat ccgctttccc gctatcgttt ttctgcggaa
     4081 cctgctgcgt gcggatcatg aaaatcctgc attgggcgtg caacgtgtcg ccctgcgcgt
     4141 tcatacgtat cgccgtaaaa aacaaaaggc agagaaaaag ctgtttcata ttgtcataat
     4201 gccgttgcaa cgcaaaagat acggctgaac gggagcgaac gcgcagcact tcttttcacc
     4261 ttccgccccg gtgctttgct cacatgcagc cggccggatt ggataacttt tcataaccgg
     4321 cgcccgcaga tcggctattg aaaattattt ttacgccgtg gagttcaacg ctatcctttc
     4381 cgatctcaaa aagaagaatt acaaacccgt gtatttcctc acgggtgaag agccgtattt
     4441 catcgacgtc atttccgatt acgtggaaaa aaacgtgctc gacgagaacg aaaaggaatt
     4501 caaccagacc gtactctacg gccgcgacac cgacgtgcag acgatcatcg ccgaagcgaa
     4561 acgctatccc atgatgtcgg agcgccaggt gctcatcgtg aaggaagcgc agaacgtacg
     4621 caacatcgaa gacctcgaag cgtacgtgga gaaaccgctc gacaccacgc tgctgcttgt
     4681 ctgctacaaa tacaaaacgc tggataagcg taaatcgttc ggaaaaacgg tcgcgaagca
     4741 cggcgtgctg ttcgaatcgg aaaagctgcg cgattacaag atccccgaat gggtgagcac
     4801 gtacctgaag gagaaaaatt attcgatcgg tccgaaagcc tcgcagctgc tcgccgaatt
     4861 tctcggcagt gacctcagca aaatctccaa cgagctcgac aagctcatga tcaacctgcc
     4921 gcccggaact gagatcaccg cgcagcacat ccaggacaac atcggcatca gcaaggacta
     4981 caacactttc gagctgagcg acgcgctctc caagcgcgat gtcgtccgcg ccaaccgcat
     5041 cgtaaattat ttcgcttcca attcaaagga tcatcccatg cccgtcacca tcgcggcgct
     5101 gtacaattac ttcgtaaaag tgctgcggta ccacgggctg gaaaacaaag ccaaagattc
     5161 cgtggcgcgt gcgctcggcg tgaatccttt ttttgtcaac gactatgcgg cagccgcgcg
     5221 gaattacccg gtcggaaagc tgaaagccat tttttcattt ctccgggaat acgacctgcg
     5281 ctcgaaaggc gtggacaacg ccagcgccga agacggggac ctgatgaagg aaatggtgtg
     5341 gaagatcctg cactgagaac aggcaggtgc gttaacgtct tccgggaata cggtctttgg
     5401 gattgaccga gctgtatgaa aacgggatat cgacgatctc cgaaaatgtt tccccgtggt
     5461 ccttcatctc ttcgtcatcg gaataatact cccacgtaac ggaaagcggt atccttttct
     5521 ggtctttcac cacgcgcatg taatcgagaa atgccttgga ggaaagcgtg ttcatgtatt
     5581 ccaggcggaa tgtaaacgaa acgcccgtca cgttttcggg gtccagcgcc tcgatgtaat
     5641 cttccagcgg ccggaaaaaa acgtaagcgt tctcagggat cgacacaccg atgatccgta
     5701 ttgcgctcgt tttctcgtca acctctacca gtggcgtgtg tcttgtcgct tcgatccgca
     5761 tatttttttc tgtgtttcgg gggtagccag accaaaaata taatttagtt tgtaaaatga
     5821 tccattccgg atgaattgtt ttaaaaaaat cagaaaatga actactggct tgtaaaatcc
     5881 gaacccgcaa aatattcctg ggaaaaattt ctcgctgacg gtaaaacgtt ctgggacggc
     5941 gtgcggaatt atgccgcaag aaatcacctg cgcgcaatga aaaaaggcga cctcgtattt
     6001 ttttaccaca gcaacgaagg actcgagatc gtagggatcg ccaaagtggt caaggaagct
     6061 tacggagatc ctaccacgaa cgaagcggca tgggtggtgg tggaccttgc gccgttcaaa
     6121 acgctgaaaa aaccggtgac gctcgcggaa atgaaaaaag aaaaagtcct caagaacata
     6181 cctctcatac gcatcccgcg cctgtccgta atgcccgtaa gtaaagagga atatgaacgc
     6241 atcatggaaa tggcttcctg agccggatat cctgtatttg aaacctcctg tttccggtgg
     6301 taaaccgcct gtcaatcgga tgtcgggcga aagggcttaa atcttacttt tgcccgaccc
     6361 cgccttaatg cacaccttac atgcgtaaag taattacccg ggcattactt gctcttctca
     6421 ttcttccgtt taccgccgcc gcacagacgg ctaccatccg cggcactgtt tccagcagct
     6481 cgaacggtga agccgtgctg tttgcgctcg tttacctgga aggaacgcct tatggtgttc
     6541 agacggacct caacggactt tattcgctga cgaaaattcc gcccggaacc tatacgctcg
     6601 tggtgcatga gtcgagcttc aatccctaca aaatgaccat caccgtaaaa gcgggcgaca
     6661 tcattacgca gaacgtttcc ctcgagccgc gcgtcatgaa ggtggttgtc atttccgcgc
     6721 atcatcagga tgtacagaat aacccggacg tcggcaaaac gtcgatggac ccgaaagaca
     6781 ttaaccgcgt tgttgcgatc ggcggtgtgc ccgacgtggc gcagtcggtg caggtgctcc
     6841 cgggtgttgt cagctcgggc gaccagggcg gacagcttta tatccgtggc ggaacacccg
     6901 tgcagaacaa agtccttctc gacgggatgg tgatctacaa tcccttccac tccatcgggc
     6961 ttttctccgt tttcgatacc gacatcatca agaacctcga cgtttacacc ggcggtttca
     7021 atgcggacta tggcggacgg atctcctcgg taatggacat caccacgcgc gacggcaaca
     7081 aaaaacattt cgcggggaaa gcgacggtta gccctttcgg tgccaagctg ctcctcgaag
     7141 gtcccatccg caggatggac gacgatccga agagcgggta cggcgccatc acctacgtcg
     7201 tttcggccaa aaactcgtat ctcgcgcaaa cgtccaagct cctgtacagc tacgtcgatc
     7261 cgaacgggat c
//
LOCUS       DASTSF010000358        14016 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1084851,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000358 DASTSF010000000
VERSION     DASTSF010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 14016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14016
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1084851"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..934
                     /locus_tag="VFU15_10190"
     CDS             <1..934
                     /locus_tag="VFU15_10190"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEU4718196.1"
                     /translation="PFKTSVGDFIMCFIVDITQRKKNEIELNLAHERLKQTSDALSSL
                     NAELENKVRERTEELADAINRLADSKVEVMRALEREKELNELKSRFVTTASHEFRTPL
                     GTILSSASLIARYEEPGDAEKRKKHTERIKAAVGNLTEILNDFLSLEKLEEGIVRSRP
                     EKTDFSQLVREVTDDMKGISKKGQDIILEYKGDREISIDRQLIRNAMINLIANAIKYS
                     PEKKPVHVHITVGKARLQMSVADQGIGIPDEDQENIFERFFRAKNTINIQGTGLGLNI
                     VKKYVELMDGKISFTSKLNEGTTFNVEIPLNENP"
     gene            931..1989
                     /locus_tag="VFU15_10195"
     CDS             931..1989
                     /locus_tag="VFU15_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013930826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU4718197.1"
                     /translation="MKKILLIEDNEDMRENTAEILGLANYKVLTAPNGKEGVRLAQAE
                     NPDLIICDIMMPELDGYGVLHMLSKNPDTAAIPFIFLTAKAEKADHRKGMSMGADDYL
                     TKPFDDMELLSAVEVRLKKNEQMQSRFSRNVEGLNKFLDEAQTISDLVKLSGNRKTKT
                     FRKKDVIYQEEAFPAGVYFLNKGKVKTSKINADGKEFITALINEGEFFGYAALLEEQP
                     YSDSAVALEESELFIIPKEDFYSLLYGNREIASGFIRLLSNDVKDKEERLLRLAYNSV
                     RKRVAEALLLLRQRYSEGKKDPFSMAISREDLANLAGTAPETVIRTLSDFKEEKLVDV
                     KGSTITITNVEKLERMKN"
     gene            2088..2843
                     /locus_tag="VFU15_10200"
     CDS             2088..2843
                     /locus_tag="VFU15_10200"
                     /inference="COORDINATES: protein motif:HMM:NF023580.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HEU4718198.1"
                     /translation="MELPRSIPPGLRFRILFLHPFMLAAVLPVFMGVVFTVVFSSMTS
                     FDVFKFKGGEPRVKAVITQVRRTNDKENSRYLYAYDYAYQLQDSVMRTGTSYDFHRDL
                     PSDTVDVEYIPGSPDISRIAGMRISPFHPALALSGLLPALIGIVFFFIAFAKGKKNLY
                     LVRNGILTEGKVISKERTSTRINNRYVYRITFEFMAGGTPVQAVTRTHLVDRLLDEAH
                     EKVVYDPNDPSKANFVDAMPKSVRKFFDGESVQ"
     gene            complement(2844..3767)
                     /locus_tag="VFU15_10205"
     CDS             complement(2844..3767)
                     /locus_tag="VFU15_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEU4718199.1"
                     /translation="MSLLVVGTVAFDAIETPFGKTDKIIGGAATYISLAASYFTKNIN
                     LVSVVGDDFPADSLEMLKKHGVALDGLQIKKGQKSFFWSGRYHTDMNSRDTLITELNV
                     LADFDPIVPENCREADFLMLGNLMPSVQQKVISQLKKRPKLIVLDTMNFWMDTAIDEL
                     KKTIAQVDVLTINDGEARQLSGEYSLVKAAQKILAMGPKVLIIKKGEHGALLFNKEQV
                     FFAPALPLEDVFDPTGAGDSFAGGFIGYLADTKDISFDNMKRAIIFGSAMASFTVEKF
                     GVERLIGLTQEEVDERVQEFIDLVQFDISLV"
     gene            3885..3958
                     /locus_tag="VFU15_10210"
     tRNA            3885..3958
                     /locus_tag="VFU15_10210"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3919..3921,aa:Asn,seq:gtt)
     gene            complement(4105..5520)
                     /locus_tag="VFU15_10215"
     CDS             complement(4105..5520)
                     /locus_tag="VFU15_10215"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEU4718200.1"
                     /translation="MKPLYLKQSQKFKNLHVQCRACNALIDTGICKKTGRSVLAKDGC
                     PSPEKHVFKAVLHVAKTKNGRKTKILQSRDYDEAIKELLAFKASLKEAEAVETTAAIA
                     ALNEAHAPPKSEAKQQPMKLFSAMSRYVGYLSGDSTTPAFKRKQRSPSHIADVERSFL
                     RLVTAAKENGREVEALTVEDMNDDELLGEVYEYLVDELELGNSAYNRAITNFVSFYNW
                     LKAEGHTTRNPWEGIVRRQTRKNVETIEPEQFDALCEIMQKPELGVRTLANGVRKNYW
                     RSFLPDAMRLLLFTGKRRTECVLMKWSDAVYKNDILQYIQVPDYKVNRQKNLKEEDWI
                     YHYAPGTEELRDLLKALGEEKYRDSDHYILAPEERMSRTTMASLLSKAFDHYAEQLGL
                     DLTLGSLRRTNFSSLAAAIGLENAQTVSGHAGMEVMKRHYISKKVLIQAATHNVFDRT
                     KARTSELNAHRGEKGEQSIER"
     gene            complement(5523..5915)
                     /locus_tag="VFU15_10220"
     CDS             complement(5523..5915)
                     /locus_tag="VFU15_10220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718201.1"
                     /translation="MPLNVEIASHITLWEIARILGYKSLRSVRRWCKTNGVQILTLGG
                     REKFVLLTEFRMKYLGTTITYLRSQYGEKWREALEAHLKDDVVGLMTVLKSSKKHHTS
                     QEAVEKRVQGENEKRFAEKLSQLAAKAS"
     gene            6150..6836
                     /locus_tag="VFU15_10225"
     CDS             6150..6836
                     /locus_tag="VFU15_10225"
                     /inference="COORDINATES: protein motif:HMM:NF016430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU4718202.1"
                     /translation="MINQMEDQIYSLLLVLKNKGDEQLIDEIFNLANRIYLKRLVAYV
                     HYMGLLEDECHDVVQNVLTEFLLRVRSRTYDFHSSGKFEAYLFTVTKNQALKYLRDNK
                     KHRDTFILSAPAKGDGENKDDVGSRIIDSEDSFAKHLNGLEIRNLFEICLQKLSRKLS
                     VVLDFLMKTDGKPTKEEIEAFIAQEKITEKAYRKRVERVKDDLVERMPSLTKMRKRGR
                     KRPDSKRRKK"
     gene            6833..7138
                     /locus_tag="VFU15_10230"
     CDS             6833..7138
                     /locus_tag="VFU15_10230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718203.1"
                     /translation="MKKKSNFDALPDEEVLRCIAEEFGCSVDTVKRILANPDLDAKTY
                     LELLLTDAGFKNDQIGRLLLSDPQFAEEYITKHKIDTSIYNDVTLEKIKARIESSKR"
     gene            complement(7379..8332)
                     /gene="dcm"
                     /locus_tag="VFU15_10235"
     CDS             complement(7379..8332)
                     /gene="dcm"
                     /locus_tag="VFU15_10235"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF012372.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HEU4718204.1"
                     /translation="MKYLSLFSGIGGFELGIEHAFAKQKQKAVCVGFSEIDKYAAHVY
                     AHHFPTHKNFGDITKIKANSLPDFDLLVGGFPCQSFSIAGKRKGFEDPRGQMFFHIAR
                     ILRAKRPRRFLLENVAGILSHNEGATFRKIICEIEKLGYSVEWHVLNSADYLPQHRTR
                     VFIVGHLGKKRAGKVFPLERTVAKAHGTHQPKTRSGIVTLYDGGFVDRKIISPQGISP
                     AIVATPKSIPNVAVSNSSNREFGWRTNYCPALTASDSKSMKIVRIGCRIRKLLPVECA
                     RLQGFPDSWCDGLSDSQAYRCYGNAVTTNVVSAIVSKMYAR"
     gene            complement(8365..8583)
                     /locus_tag="VFU15_10240"
     CDS             complement(8365..8583)
                     /locus_tag="VFU15_10240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718205.1"
                     /translation="MKERNTQTRKGEKKQDPEHLKEKHLRIITRNFLYALHKRQNCCE
                     VCRQCRQAIIDESMRFAELYALITTDTE"
     gene            complement(8592..9014)
                     /locus_tag="VFU15_10245"
     CDS             complement(8592..9014)
                     /locus_tag="VFU15_10245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718206.1"
                     /translation="MLYYTTPVPRAVFDLIPALNESELKVLLVVIHKTLGWKSEKTHT
                     GRKECDWISGKQFQKGTGLSDRAISKATASLVAQKWLIVMDDCGKVLETPQERKGKTR
                     LYFRLAQALMPATENYSQVIRTKIGETANIFRITKHYS"
     gene            complement(9039..9263)
                     /locus_tag="VFU15_10250"
     CDS             complement(9039..9263)
                     /locus_tag="VFU15_10250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718207.1"
                     /translation="MPVRIFSVCTKIVFNTKEDGKERVKWYRAGTLKMLDSGQVYLKM
                     FHQPQVDFVCFEQKQEQDERELPVIQVDKQ"
     gene            complement(9519..10460)
                     /locus_tag="VFU15_10255"
     CDS             complement(9519..10460)
                     /locus_tag="VFU15_10255"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEU4718208.1"
                     /translation="MYEKIEKYLAWKATHHRPASVAYRRSLLRFVEMCGDKEIEQYTI
                     QDVVLFTNLLAERFANETVRFALAIIKNFFHFYNEQNYKCLSPWLIEIPRQLPPNSFR
                     AIEKREVQEMVSIISADEFTSLRALVIILMLWDTGVRVSELRDLNVSDIDQEKRRAII
                     STKKAFQARIILWSEATNNYLRTYLHERGKIIGITSIDSPVFIGRKGKEKWKPRRLTT
                     KSIQRIVKAYGNEANVTRKVTPHRFRHGWAHTRRDAGASLPFIQRGLGHKNAISSMRY
                     QQYSDPEFEMTARKHLKTPVIASPYTTLDFQQITVKK"
     gene            complement(10714..11031)
                     /locus_tag="VFU15_10260"
     CDS             complement(10714..11031)
                     /locus_tag="VFU15_10260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718209.1"
                     /translation="MLSQKDIELLSSVFATKDDMQKLASNEDVKFLRSNFQDVLEGMD
                     KMGEKTEKNFDEVRKDINELREMVQETITVGDKEKEVLKTTTAKHEKWIKQLAGNADL
                     ALQ"
     gene            complement(11147..12751)
                     /locus_tag="VFU15_10265"
     CDS             complement(11147..12751)
                     /locus_tag="VFU15_10265"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEU4718210.1"
                     /translation="MQPFKHIKANRLFCLIILVTIGMRSPLLAQPVSFHTQYGTASQR
                     EYAVQMIQCRDSGFLALAWRGGFGYEALALRIGNTGNIIWQRSLKDTSSQGLYPSRCI
                     ETQDGGFCILASPRYGSLPTLTFSALIKIDNTGGLAWTTVVDDSLDYALVGKDLAEAA
                     DGSIYVFGNRIPPCPGPCPEYVYLMKFNSAGQCLWYESSQQTGYQASRILLRSDSLPA
                     LGFSDQQRSFITNTNSSGMPLGTHSYTASSGTSTALSDFIMGPDGKIRCIQFSDISGG
                     AILSAFDSLETLTNSRRVTKANRTISGIQVLMTDTGYVSLLKINDTAYSLITDWSDGM
                     NYPNGFFVSALDPFYPFSSTRKSDGSIFIYGSLYSPSASFPDVHFVKTDIATTAQMPL
                     SGCSLDSTSLNISAVTMLDSITSVNFVPTAFVLKGNYVTDSVANLVETSDCFATGINA
                     LAMDKDFVLVYPNPASSSITFQMPNASGTIVILDQLGREVKRQQLNGEKQVAISVADL
                     ADGIYFYRITDSKSSSEQGKFVVQHQ"
     gene            complement(12800..13051)
                     /locus_tag="VFU15_10270"
     CDS             complement(12800..13051)
                     /locus_tag="VFU15_10270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718211.1"
                     /translation="MHTLITVTKIASAYVDLLHNTPTQINADHISKLVPLSHVQGIGS
                     TVIFFRDGSTMNVLETVGEVKSRVKEEQERYFEFGFGKN"
     gene            complement(13350..>14016)
                     /locus_tag="VFU15_10275"
     CDS             complement(13350..>14016)
                     /locus_tag="VFU15_10275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718212.1"
                     /translation="ENLRNTDQRRLSYNLESIMDINFSGKGNWANAPIHNVLLSLIRK
                     RPRDMVKLCTLAAKKARKENSQKIKTKHWEASFEEYSQGVIHDTIVEYKSELRDIERL
                     IFGMKPDKKGRLASEGFNYTTPTLKLKLNKIIERGKFMFANGNPATAQELSQFLFKIN
                     FLTAWKRLDDGKILRKYFEENNYLSTSFVDFGFNWEVHLAYRWALQPDSLEDIFAKFA
                     KEE"
BASE COUNT         3478 a         3371 c         3422 g         3745 t
ORIGIN      
        1 cccgttcaag acatccgttg gcgatttcat catgtgcttc attgtcgaca ttacccagcg
       61 caagaagaac gagatcgagc ttaacctggc gcacgaacgg ctcaagcaga catcggatgc
      121 gctcagctcg ctcaatgccg agctggaaaa taaagtcagg gaaagaacag aagagctcgc
      181 ggacgccatc aaccgcctgg ccgattcgaa ggtggaagtg atgcgcgcgc tcgaacgcga
      241 aaaagagctg aacgagctga aatcccgttt cgtgaccacg gcttcgcatg aattccgcac
      301 accgctggga acgatccttt cctcggcgtc gctcatcgcg cgttatgaag aaccgggcga
      361 tgcggaaaaa aggaaaaaac atacagagcg catcaaggcc gccgtgggaa accttacgga
      421 gatcctcaac gattttcttt cgctggaaaa gctggaggag ggcattgtcc gcagcaggcc
      481 ggagaaaacc gacttcagcc agctggtgag ggaagtgacc gacgacatga agggcatttc
      541 caaaaaaggg caggatatta ttctcgaata caaaggcgat cgcgaaattt ccatcgacag
      601 gcagcttatc cgcaacgcga tgatcaatct tatcgccaac gcgatcaaat attccccgga
      661 aaaaaagccc gtgcatgttc acatcacagt gggaaaggcc cggctgcaga tgtcggtggc
      721 cgaccagggc atcggtatac cggatgagga ccaggaaaac atattcgagc gtttcttccg
      781 ggccaagaac acgatcaata tccagggaac gggtctcgga cttaacattg taaaaaaata
      841 cgtggagctg atggacggga agatctcttt tacaagtaaa ttaaatgaag gcactacatt
      901 taacgtcgaa ataccgctaa acgaaaaccc atgaaaaaaa tcctgctcat cgaagacaat
      961 gaagatatgc gcgagaatac cgccgagatc ctcgggctcg ccaactacaa agtgctgacg
     1021 gcgcccaatg gaaaagaagg cgtccgtctt gcacaggcag aaaatcccga tcttattatc
     1081 tgcgatatca tgatgcccga gctggacggt tacggcgtgc tgcacatgct gtcgaaaaac
     1141 ccggatacgg cagctattcc gtttatcttt cttacggcga aagcggaaaa ggccgatcac
     1201 cgcaaaggaa tgtccatggg cgcagatgat tacctcacaa agccgttcga tgacatggaa
     1261 ttgctcagcg ccgtcgaggt gcggctgaaa aagaacgaac agatgcagtc gcgtttctcg
     1321 aggaacgtcg aaggactcaa caaatttctc gatgaggcgc aaaccatcag cgaccttgtc
     1381 aagctgtcgg ggaaccgcaa aacgaaaaca ttccggaaga aagacgtcat ttaccaggaa
     1441 gaagcattcc ccgcgggcgt ttatttcctc aacaagggaa aggtcaagac cagcaagatc
     1501 aacgcggacg ggaaggaatt cataaccgcg cttatcaatg aaggcgaatt cttcggctat
     1561 gccgcgcttc tcgaggagca gccgtacagc gattccgccg tggcgctcga agaatcggag
     1621 ctgttcatca tcccgaagga ggatttctat tcactgcttt acggcaaccg cgagatcgct
     1681 tccgggttca tccgcctttt gtcgaacgac gtaaaggaca aggaagaacg cctgctgcgc
     1741 ctggcgtaca attccgtgcg caagcgcgtg gctgaagcgc tgctgctgct gcgccagcgt
     1801 tattccgaag gaaaaaaaga tccgttcagc atggctattt cgcgggaaga tcttgccaac
     1861 ctcgcgggaa cagcgcccga aacggtcatc cgcacattat ccgatttcaa ggaagaaaag
     1921 ctcgtcgacg tgaaaggcag caccatcacc atcacgaatg tcgagaagct ggaacggatg
     1981 aagaactgaa aaaccggggt tgcctgcgcg gaaccgatcc ggcgcaaaaa aggcatcttt
     2041 cataaattac cgactgcatt cggccgatgt tcacttcctc tgcaaacatg gaattgccgc
     2101 ggagcatacc gccagggctt cgcttcagga tactcttcct gcatcctttt atgctggccg
     2161 cggtgctgcc cgttttcatg ggcgtggtct tcaccgtggt tttttcatcc atgaccagct
     2221 tcgatgtttt caaattcaaa ggcggggaac cgagggtgaa agcagtgatc acgcaggtcc
     2281 gccggacgaa cgacaaggaa aattcgcggt atctctatgc gtatgattat gcctaccagc
     2341 ttcaggatag cgtaatgcgt acgggcacgt cgtatgattt tcacagggat cttccgtcgg
     2401 atacggttga cgtggaatat attcccggca gcccggatat ttcgcgcatt gcaggcatgc
     2461 gtatttctcc ttttcacccg gccctggcgc tttcaggact tctgccggct ctcatcggca
     2521 tcgttttttt ctttatcgca tttgcaaagg gaaagaagaa cctttacctc gtgcgcaacg
     2581 gcattctcac ggagggcaaa gtgatttcga aagagcgcac cagcaccagg atcaacaacc
     2641 gctatgtcta ccggatcacg ttcgaattca tggccggcgg aacgcccgtg caggcggtaa
     2701 cgcgaacgca cctggtggac cggttgctgg atgaggccca tgaaaaagtg gtgtacgacc
     2761 cgaatgatcc gtcgaaagcg aacttcgtcg acgccatgcc gaaaagcgtg cggaaatttt
     2821 ttgacgggga gagcgtgcag taattacacg agcgaaatat cgaactgcac gaggtcgatg
     2881 aactcctgca cgcgttcgtc cacttcttcc tgcgtaagcc cgataagtct ttccacgccg
     2941 aatttctcga cggtgaacga cgccatcgcc gatccgaaaa tgatcgcacg tttcatgttg
     3001 tcgaaggaaa tgtctttcgt gtccgcgaga tagccgatga agccgccggc aaagctgtcg
     3061 cctgcgcccg taggatcgaa cacatcctcg agcgggagcg cgggcgcaaa gaacacctgt
     3121 tccttgttga agagcagcgc gccatgctcg ccttttttga tgatgagcac tttgggtccc
     3181 atggcgagga tcttctgcgc cgctttcacg agcgaatatt cgcccgaaag ctggcgcgct
     3241 tcgccgtcgt tgatcgtcag cacatcaacc tgtgcaatgg ttttcttcag ctcatcgatg
     3301 gcggtgtcca tccagaaatt catcgtgtcg agcacgatga gcttcgggcg ttttttcagc
     3361 tggctgatga ccttctgctg cacggaaggc atgaggttgc cgagcatgag gaaatcagct
     3421 tcgcggcaat tttccggcac gatgggatca aaatccgcga ggacgttgag ctcggtgatg
     3481 agggtatcgc gcgaattcat gtcggtatgg tagcgccccg accagaaaaa ggacttctgt
     3541 ccctttttga tctgcagtcc gtcgagcgca acgccgtgtt ttttcagcat ttcgagcgaa
     3601 tccgccggga aatcgtctcc cacaacagaa acgaggttga tgtttttcgt gaaataggag
     3661 gctgccagcg aaatataggt ggccgccccg ccgatgatct tgtccgtttt gccgaaaggc
     3721 gtctcaatgg cgtcaaaagc cacggtaccg accactaaaa gactcatagg gaatggttaa
     3781 agttttgggc aaagttattg catatttcat tcgtacgtgc taatattgca acccgttttt
     3841 agaaacggcc gttcgttctt caaccgacta aaaccagaat atattcctcc ttagctcagc
     3901 tggttagagc acatgactgt taatcatggg gtccctggtt cgagcccagg agggggagca
     3961 agagataaaa gcctgactca gtttgagttg ggcttttctc gttttgggac ttccatgatc
     4021 gagcgcggag aaaattggaa tcagcgcgga atttcagcgc ggaacttttg aaaatcgctt
     4081 gtaaaagatt gttttgtgtc gaagctatcg ttctatcgac tgctcgcctt tctcgcctct
     4141 atgcgcgttt aattcgcttg tacgggcttt tgtcctgtcg aacacattat gcgttgcggc
     4201 ctgaatcaaa actttcttgc tgatgtagtg ccgtttcatc acctccattc ccgcgtggcc
     4261 gctcacggtc tgtgcatttt caagcccgat ggcggcggca aggcttgaga agttggtgcg
     4321 acgcagcgat ccgagcgtga ggtcgaggcc gagctgttcc gcgtagtgat cgaatgcttt
     4381 ggaaagcagg ctcgccatag tggtgcgaga catgcgttct tccggtgcga gaatgtagtg
     4441 atcgctgtcg cggtattttt cctcgccaag cgctttgagc agatcgcgca attcctccgt
     4501 tcccggtgcg taatggtaaa tccaatcttc ctctttcaaa ttcttttgcc ggttcacctt
     4561 gtaatccggc acctggatat attggagaat atcattcttg tacaccgcgt cgctccattt
     4621 cataaggacg cattcggttc tgcgcttgcc tgtaaagaga agtagtcgca tcgcatccgg
     4681 caggaagctt ctccaataat tttttcgtac tccatttgca agcgtgcgca cgccgagctc
     4741 cggtttttgc atgatctcac aaagggcatc gaattgttcc ggctctatcg tctcaacatt
     4801 tttcctggtt tgccttctca ctattccctc ccacggattg cgcgtggtat gtccttctgc
     4861 cttaagccag ttatagaacg agacgaaatt ggtaattgcg cgattgtacg cactgttgcc
     4921 gagttccaat tcgtcaacca ggtattcgta cacttcgccg agtagttcgt cgtcattcat
     4981 gtcttcgacg gtaagtgctt cgacttcgcg gccgttttct tttgcggcgg tgacaagccg
     5041 gagaaagcta cgctcaacat cagctatatg agagggtgac cgttgcttgc gcttgaaggc
     5101 aggagttgtt gagtccccag agaggtagcc gacatagcgg ctcatggccg aaaacaattt
     5161 catcggttgc tgcttcgctt ctgattttgg cggtgcgtgc gcttcgttga gtgctgcaat
     5221 ggcagcggtc gtctcgacag cttccgcctc cttgagcgat gccttgaatg caagcaattc
     5281 tttgatcgct tcatcgtagt ccctgctttg gagaatcttt gtcttgcggc cattcttggt
     5341 ttttgcgacg tgcaaaacgg ctttgaaaac gtgtttttct ggcgaagggc aaccgtcttt
     5401 ggcaagcacc gaacggccag ttttcttgca aatgccggtg tcaataagcg cattgcatgc
     5461 gcgacactgg acatgcaggt ttttgaactt ttgagattgt ttgaggtaga gtggcttcat
     5521 ggttatgatg cctttgctgc taattggctt aacttctctg caaaccgttt ttcgttctcg
     5581 ccttgtacgc gtttttcaac agcctcttga ctcgtatggt gttttttact acttttcagt
     5641 accgtcatta gacccactac atcatccttg agatgagctt ccagcgcctc gcgccatttc
     5701 tccccatatt ggctgcgtag gtatgtaatt gtcgttccca ggtacttcat tcggaactct
     5761 gttagaagta caaacttttc cctaccacca agcgttagta tttgcacgcc gttggttttg
     5821 caccaacggc ggacgcttcg tagacttttg tagccgagga tgcgggcaat ttcccataat
     5881 gtaatatgac tggctatttc aacgtttagt ggcataaaag gcatttctga gaaatacctt
     5941 atggcaattt tctatgacag gaaaaaaaac tttatagaaa acgtcacata tgaacacgat
     6001 aatggtattt actactgata ccccatcaaa gtcaaaccta aaaaagcagc ttatggaaca
     6061 aacgaatgca tcgaatcact ctccaaacac cgaatggttc atgcggttag gaacgagcac
     6121 cgcattgaag aagctccccc aacatctaaa tgatcaatca aatggaagat caaatttact
     6181 ctctgctgct cgttctgaaa aacaagggcg acgaacagct aatcgacgaa atttttaatc
     6241 ttgcaaaccg aatctacctc aaacggctcg tagcttatgt gcattatatg gggctactcg
     6301 aagatgaatg tcatgatgtt gtgcaaaatg tactaacaga atttcttttg cgtgttcgaa
     6361 gcagaactta tgattttcat tccagcggaa agttcgaagc ttaccttttt acagtcacaa
     6421 agaatcaagc tttgaaatat ttgcgcgaca ataagaagca ccgggacaca tttattttgt
     6481 ctgctccagc aaaaggagat ggagaaaata aagatgatgt gggaagccga attatcgaca
     6541 gtgaagattc ttttgcgaag catttgaacg gccttgaaat ccgaaacctg tttgaaatat
     6601 gcttgcagaa actttcccgg aagctctccg ttgtgcttga tttcttaatg aaaacagatg
     6661 gcaaacccac taaagaagaa atcgaagcat ttatagcaca agagaaaatt acggagaaag
     6721 cgtatcgtaa aagagttgag cgggtaaaag atgacttggt agaacggatg ccgtcattga
     6781 cgaaaatgcg aaaaagaggt agaaagaggc cggactcaaa acggcgaaaa aaatgaaaaa
     6841 gaaatcgaat ttcgacgcac tacctgatga agaagtactg cggtgcatag cagaagaatt
     6901 tggctgctcc gtagatacgg taaaaagaat tcttgctaat cctgatcttg atgcaaagac
     6961 atatttggaa ttgctcttaa ctgatgcggg tttcaaaaat gatcaaatcg gacgacttct
     7021 gttaagtgat cctcagtttg ctgaggaata catcacgaaa cacaaaatcg acacgagcat
     7081 ctacaatgac gtaaccttag aaaagatcaa ggcaagaatc gaatcttcta aacggtaagt
     7141 tgtttaacta cttatgttct accatgaggt tatgagcttc attggttata gcacctgttt
     7201 caggtgcttt ttttatgtgg tcatgccgtc tcacatcggc caccacacct ccttttccct
     7261 cgatattcga ccgcatgcgt ttatgcagag ccgcaagagc atttcaattt ctcggtcgct
     7321 actcctcctg gctgtcgcct ggagcgtgct cggtcgtaat ctcatgcact tgcggaaatc
     7381 agcgcgcgta catcttactc acgattgccg acaccacatt tgttgtcacg gcgttgccat
     7441 aacaacggta tgcttgggag tcagaaagtc cgtcacacca cgaatcagga aaaccttgca
     7501 ggcgcgcaca ttcaaccggt agcagctttc taatccggca gccgatgcgc acaatcttca
     7561 tactcttgct gtcggatgcg gtgagcgccg ggcagtagtt ggttcgccaa ccgaactcgc
     7621 ggttactgct gtttgatacc gcaacgttcg ggatcgactt cggtgttgcg actattgccg
     7681 gactgatccc ttgcgggctg attatttttc ggtcaacgaa tccgccgtcg tagagggtaa
     7741 caatgccaga ccgcgttttc ggctgatgcg tgccgtgagc tttcgcaact gttcgttcga
     7801 gaggaaatac ttttccggcg cgtttttttc caagatgtcc gacaatgaat acgcgggtgc
     7861 gatgttgcgg cagataatcg gcgctgttaa gcacgtgcca ttcgacactg taccccagct
     7921 tttcaatttc acagatgatt tttcggaacg tagctccttc attgtgtgac aagatgcctg
     7981 cgacattctc aagcaggaat cgtcgaggtc gttttgctcg cagtattctc gcaatgtgaa
     8041 agaacatttg cccgcgggga tcctcgaagc ctttgcgctt ccccgcaatg ctaaagctct
     8101 ggcatgggaa cccgccgacg agcaggtcga agtcggggag cgaatttgcc ttgatcttcg
     8161 tgatgtcccc gaagtttttg tgtgttggga aatggtgtgc atatacgtgt gctgcgtatt
     8221 tgtctatttc ggaaaaaccg acgcagacgg ctttttgttt ttgctttgcg aacgcgtgct
     8281 cgatacccaa ctcaaaccct ccgatacctg aaaagagtga gagatatttc aagctgttgc
     8341 atcgtgatgc tgataatgcc cgatctactc cgtgtcggtt gtgatcagtg cgtagagttc
     8401 tgcaaaccgc atgctctcgt cgatgatcgc ctgcctgcac tgcctgcaca cctcgcagca
     8461 gttctgccgc ttatgcaatg catacaggaa attcctggta atgatccgca aatgcttttc
     8521 cttaaggtgt tccgggtcct gcttcttttc cccttttctt gtttgtgtat ttctttcttt
     8581 catgggtttc tttatgagta atgctttgtt atccgaaaga tgttcgctgt ctcaccgatc
     8641 tttgttcgga tgacctgcga ataattttcg gtggcaggca tgagcgcctg ggcgagccgg
     8701 aagtacagcc gcgtctttcc cttgcgttcc tgcggcgttt caagtacctt gccgcaatcg
     8761 tccataacga tgagccactt ttgcgcaaca agcgaagcgg tcgccttgct gatcgcgcgg
     8821 tctgataatc ctgttccctt ttgaaactgc ttgccagaaa tccagtcaca ctctttcctg
     8881 ccggtgtgtg tcttttcgct tttccagccg agcgttttgt ggatgacgac gagcaacact
     8941 tttagttccg attcattcag cgccggaatg aggtcaaaaa ccgcacgcgg cactggcgtc
     9001 gtgtagtaca acatctttag aaaaacttta gaaatatttt attgcttgtc cacttggatc
     9061 accggcagct ctcgttcgtc ctgttcctgc ttctgttcaa aacaaacaaa atcgacctgc
     9121 ggctgatgga acatttttag atacacctgc ccgctatcta acattttgag tgttccggct
     9181 ctataccact ttacccgctc cttgccgtct tcctttgtgt tgaagacgat ttttgtgcac
     9241 acagagaaaa ttctgactgg catttttctt ttgtttaaaa tagtaatgtc tcgtctatga
     9301 ccgcgtgtgc aatctgcttt caaaatctca aatgtcccat aagtctaatt gtgcgacatt
     9361 gagatggcgg agaaaatcgg gcgttagccc gtgttccctt acagatgctc acgataggtt
     9421 ataaatgagg ttctgcttac tatatcccgt ctctttgttt tgtgcaacct tgttttccac
     9481 agttgcataa aaccgccatt aacgggcggt tttatcggct atttcttaac tgtgatctgc
     9541 tgaaaatcta acgtagtata tgggcttgca attacaggtg ttttcaggtg ctttcgtgct
     9601 gtcatttcaa actctggatc ggagtattgc tggtaacgca tggaagagat ggcgttttta
     9661 tgacctaaac ctctttggat aaacggcaat gatgctcccg cgtcacggcg cgtatgcgcc
     9721 cacccgtgac gaaatcggtg cggcgtcact tttctcgtaa cgttcgcctc gtttccgtac
     9781 gctttgacaa ttcgttgaat acttttggtc gtcaatcgtc gtggtttcca tttctctttc
     9841 cctttccgcc ctatgaatac cggactgtcg atgcttgtaa ttcctatgat ctttccgcgc
     9901 tcatgcaagt aagtacgcag gtaattgttt gtcgcttccg accaaagtat gatgcgtgcc
     9961 tgaaatgctt ttttggtact gatgatcgca cggcgttttt cctggtcgat gtcgctgacg
    10021 ttcaagtcgc gcaattctga aacacggact cccgtgtccc acagcatgag aataataact
    10081 aaagcgcgca gcgaggtaaa ctcgtcggca gaaataatgg aaaccatttc ctgcacttcc
    10141 cttttctcga ttgccctgaa tgaattgggc ggcagttgtc gtggtatctc aatcagccat
    10201 ggcgaaaggc atttgtaatt ctgctcgttg tagaagtgaa aaaagttttt gatgatcgcc
    10261 aatgcaaaac gcactgtttc atttgcaaac cgctctgcaa gcaggttcgt gaaaaggact
    10321 acgtcctgga tcgtgtactg ttctatttcc ttgtcaccgc acatttccac gaaccgcagt
    10381 agcgaacgcc ggtatgcaac gcttgccggc ctgtggtgcg tggctttcca tgctaaatac
    10441 ttctctattt tctcgtacat aacgcataaa gcccgcctga gactaatcgg gcgggctgtt
    10501 atgctgcacg tgcgttgccg cacgtacgga aatcaacata acacataata aataaaaagt
    10561 aaagaggagg gtgttatgct acaagtccac cgttgttagt ctcaattccg gttttccgga
    10621 atgccccttt cgggcaacgg tcctgtatta atttgtaatt caacctgtat aaagtataga
    10681 gcaatcacta tccgctgtca atgatccgtt ttcctattgc aatgctaaat cggcatttcc
    10741 ggcaagttgc ttgatccatt tttcgtgttt cgcggtggtg gtcttcaaaa cttccttttc
    10801 cttgtcgccg acagtgatgg tttcctgcac catctctctt aattcattga tgtctttgcg
    10861 gacttcgtca aaattctttt ctgtcttttc tcccatcttg tccatccctt ccagcacgtc
    10921 ctgaaaattt gaacgcaaaa atttgacgtc ctcatttgag gcaagttttt gcatgtcgtc
    10981 ttttgtggca aaaaccgacg acaaaagttc gatgtccttt tgagaaagca tgttctgatt
    11041 ataccccatc gcaatatatc cgcaactccc cgtaattcac acgaaaaacc acaacaatga
    11101 gaactaaaaa accttcaaat gtcatagaag gaaatgcaaa acggcattat tgatgctgaa
    11161 caacgaactt tccctgttca gaactgcttt tgctgtccgt tattcgataa aaatatatcc
    11221 catccgccag gtctgcgacg gaaatcgcca cctgtttttc tccgttcaat tgttgccgct
    11281 tcacttccct gccgagctga tcaaggatca caatcgtacc tgaagcattt ggcatttgaa
    11341 atgtgatcga agaggaagcg ggattgggat acaccaaaac aaaatcctta tccattgcca
    11401 gcgcatttat tcccgtggca aagcagtcgg aagtttcaac gagattcgcc accgagtcgg
    11461 ttacgtaatt tcctttgagc acaaatgccg tcggtacgaa gttcacgctg gttatggaat
    11521 caagcatggt tacggcggaa atattcagcg acgtcgaatc caacgaacag ccggaaagcg
    11581 gcatctgtgc cgttgtggct atgtcggttt taacaaaatg gacatcgggg aacgaagcgc
    11641 tcggcgaata gagggagccg tatatgaaaa tgctgccgtc ggactttcgt gtgctgctga
    11701 aggggtagaa cgggtctaag gcgcttacga aaaatccgtt cgggtaattc atcccgtcgc
    11761 tccagtcggt aatcagcgaa tatgcggtgt cgttgatctt gagcagagag acatagcctg
    11821 tatctgtcat gagtacctgg ataccggaaa tggtacggtt agccttcgtc actcgtcggg
    11881 aattggtaag agtttcgaga gaatcgaatg ctgacaggat cgctccacct gaaatgtcgg
    11941 aaaattgaat gcaccggatt tttccgtccg gacccatgat gaaatcactg agcgccgtgc
    12001 tcgttccgga tgaagcggta taggaatggg tgccgagtgg catccccgag gaattggtgt
    12061 tggtaatgaa tgatcgctgt tgatcggaga agccgagtgc gggcaggctg tccgaacgaa
    12121 gcaggatgcg ggaagcctgg tagccggttt gctgcgaaga ttcataccac aggcattgcc
    12181 ctgcagagtt gaacttcatg agatatacgt attcgggaca gggcccggga caagggggaa
    12241 tgcggtttcc gaaaacataa atacttccat cggctgcttc agccaggtct ttccctacca
    12301 gtgcgtagtc cagagagtcg tcgacaacgg ttgtccaggc taagcctccg gtattatcaa
    12361 tcttgatgag cgctgagaaa gtgagcgttg gaagtgagcc gtatcgcggc gaggcaagaa
    12421 tgcagaaccc tccgtcttgt gtttcgatgc atcgcgatgg gtatagccct tgtgaggaag
    12481 tatccttaag cgagcgctgc cagatgatat tccccgtatt ccctatccgt agtgccaatg
    12541 cctcataccc aaatccgccg cgccatgcga gcgcaagaaa gccgctgtcg cgacattgga
    12601 tcatttgcac cgcgtattcg cgctgcgaag cggttccgta ttgcgtgtga aaggagactg
    12661 gttgagctaa aagaggagac ctcattccga tcgttaccag aataatcagg caaaaaagcc
    12721 tatttgcctt tatatgtttg aaaggctgca tatcccccat gctttatgaa agatacgaaa
    12781 tcgggacaca agaagatcgt tagtttttgc cgaagccgaa ctcaaagtag cgctcttgct
    12841 cttccttaac ccgcgatttg acttcaccga cggtttcaag aacgttcatc gtgcttccgt
    12901 cacgaaagaa gatgaccgtc gaacctattc cctgcacatg ggaaagagga acgagtttgg
    12961 aaatatgatc ggcattgatt tgcgttggcg tgttgtgcag aagatcgacg tacgcggatg
    13021 caatttttgt gacggtgata agcgtatgca tggtttatga tttcgctttt cttgttcaga
    13081 cgttcggtaa aagtgggtaa aaatagaaat ctcgtctgaa tacagataag tgcctttttg
    13141 gtcttttgtg ccaattttga tgctactaat gaaatgttca agggagtatt cgccgcccga
    13201 tcaatatcca tctacgagat tagcatatcc gcattcataa tttgtatctt actggggacg
    13261 ctaaccgctg tagataaatt aagagattcc tggccaacca acggttccga aaaaagatgg
    13321 ggatcaaaat attatggcat atatgatacc tattcttcct tggcgaactt tgcgaaaata
    13381 tcttctaatg aatcgggttg caaggcccaa cgataagcaa gatgaacctc ccaattgaat
    13441 ccaaaatcaa cgaacgaagt ggacaaatag ttgttctctt caaaatattt gcgcagaatt
    13501 ttgccgtcat ccaacctctt ccaggccgtg agaaaattta ttttaaacaa aaactgagaa
    13561 agctcttgag ctgttgccgg gttgccattt gcgaacataa actttccgcg ttcaataatt
    13621 ttgttaagct tgagcttgag tgtaggagtt gtgtaattaa agccttccga agccagcctg
    13681 ccttttttat cgggtttcat gccgaaaatt agccgttcaa tatctcttag ctcggattta
    13741 tattctacga tggtatcgtg gatcacacct tgtgaatact cctcaaagct tgcttcccag
    13801 tgttttgttt ttattttttg tgaattctct ttccgtgctt ttttggcggc caacgtgcaa
    13861 agtttcacca tatctcgcgg tcgcttgcga attagtgata aaagcacatt atgaatgggg
    13921 gcattcgccc aatttccttt tccggaaaag ttaatgtcca taatggattc gagattgtag
    13981 gataatcttc tttggtcggt attgcgaaga ttttct
//
LOCUS       DASTSF010000359         2306 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1139095,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000359 DASTSF010000000
VERSION     DASTSF010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2306
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1139095"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..793)
                     /locus_tag="VFU15_10280"
     CDS             complement(<1..793)
                     /locus_tag="VFU15_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009084666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU4718213.1"
                     /translation="MSKKILANDGIDTAGKALLEKAGYEVITEKVPQDKLITEINSHK
                     YVGITVRSATKVRKDIIDACSSLKFIARGGVGMDNIDVEYAKEKGLHVINTPAASSHS
                     VAELVFAHLFGAVRFLYDANRRMPVKGETEFEALKKKYSKGIELKGKTIGIVGFGRIG
                     QEVAKIALGLGMKVIAHDPFIAEAMVILDIHGHSPIKLKIETTTLDKVLEEAHFISLH
                     VPGGKMIGADQIAIMKKGVILVNASRGGVIDEHDLIEALNSGQVAH"
     gene            complement(836..1930)
                     /gene="serC"
                     /locus_tag="VFU15_10285"
     CDS             complement(836..1930)
                     /gene="serC"
                     /locus_tag="VFU15_10285"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020082010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HEU4718214.1"
                     /translation="MSSTVLTKKAHNFSAGPGILPVDVLKQAAEACINFDNLNLSLLE
                     ISHRSKNFEKVMDEARAIVKELLGLGDGWQVIYLGGGASTQFAMVPYNLLREGGSAAY
                     VNTGVWANKAIKEAKLVGNTKVIASSEDKKFAYIPKGYEIPADADYLHITSNNTIYGT
                     EMKEFPKSPVPVVCDMSSDIFSRPIDGNQFSLIYAGAQKNMGPAGATMVAVREDILGK
                     AGRKLFSMLDYQVHIKGGSMYNTPPVFPVYVCLLTLQWLKKNGGTEWIGNINKKKAEL
                     MYGEIDRNSLFTGTVIDKADRSMMNACFVMTKPELEPEFDKLWKEAGISGIRGHRDVG
                     GYRASMYNALPLDSVQVLVDVMQEFEKKFA"
     gene            complement(2033..>2306)
                     /locus_tag="VFU15_10290"
     CDS             complement(2033..>2306)
                     /locus_tag="VFU15_10290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HEU4718215.1"
                     /translation="TWKKVMEYDPVASHASWMENFPWTRISDVEQPGTPKKMIDTAHL
                     RSLSPAQIRDYLGDGNFGGAYFKPDEIMEDIWRTAVSELRTLIGKM"
BASE COUNT          475 a          613 c          616 g          602 t
ORIGIN      
        1 cgtgcgccac ctgtccgctg ttgagcgctt cgatgagatc gtgctcgtcg atgacgccgc
       61 cacgtgacgc attcacgagg atcacgcctt ttttcatgat cgcgatctgg tcggcgccga
      121 tcatctttcc gccgggcaca tgcagggaaa tgaagtgcgc ctcttccagc actttgtcga
      181 gcgtcgtggt ttcgatcttc agcttgatcg gggagtgacc gtggatatcg agaataacca
      241 tcgcttctgc aatgaacggg tcgtgcgcga tcactttcat gccgagaccg agagcgatct
      301 tcgccacttc ctgcccgatg cgcccgaagc ccacgatgcc gatcgttttt cctttcagct
      361 cgatgccttt cgaatatttt ttcttcagcg cttcgaattc ggtctcgcct tttaccggca
      421 tccggcggtt ggcgtcataa agaaaacgca ccgcgccgaa aagatgggcg aacacaagct
      481 cagccacgga atgagaagaa gctgcgggtg tgttgatcac gtgcaggcct ttttctttcg
      541 catactcgac atcgatattg tccatgccca cgccgccgcg tgcaatgaac ttcagcgatg
      601 agcaggcatc gataatgtct ttccggactt tcgtcgcgga acggacggtg atgccgacgt
      661 atttgtggga attgatttcc gtgatgagct tgtcctgcgg gactttttca gtgatgactt
      721 cgtagccggc tttttccagc agggcttttc ctgctgtatc gatgccgtca ttggcaagaa
      781 tttttttgga cataattgat tagtcgttat tcagtgggag aaaacaaaaa ccggctcacg
      841 caaacttctt ctcgaattcc tgcatcacat ccacgagcac ctgcacgctg tcgagcggga
      901 gcgcgttgta catggatgca cggtagccac caacgtcgcg gtgaccgcgg atcccgctga
      961 tgccggcttc tttccagagc ttgtcgaact caggttcgag ctccggtttc gtcatcacga
     1021 agcaggcatt catcatcgaa cggtcggctt tgtcgatcac cgttcccgtg aacaggctgt
     1081 tgcggtcgat ctcgccgtac atcagctctg cttttttctt attgatgttc ccgatccatt
     1141 cggttccgcc gttctttttc agccattgca gcgtgagcag gcagacgtaa accgggaaaa
     1201 cgggaggcgt gttgtacatc gatccgcctt tgatgtgcac ctggtaatcg agcatggaga
     1261 agagtttgcg cccggctttg ccgaggatat cttcgcgcac ggccaccatc gttgcgccgg
     1321 caggtcccat gtttttctgc gcgcccgcgt agatgagcga gaactggttc ccgtcgatcg
     1381 ggcggctgaa aatatcggat gacatgtcgc atacgacggg gacggggctc ttcggaaatt
     1441 ccttcatttc cgttccgtag atcgtattgt tggaagtgat gtggagataa tccgcgtcag
     1501 cgggaatttc gtagcccttc gggatataag cgaatttttt gtcttccgag gaagcgatca
     1561 ctttcgtatt gccgacgagc ttcgcttctt ttatcgcctt gttcgcccaa acgccggtgt
     1621 tcacgtaagc agcgctgccg ccttcgcgca gcaggttgta gggaaccatt gcaaactgcg
     1681 tgcttgcgcc tccgccaagg taaatgacct gccatccgtc gccaagaccg agcagttctt
     1741 ttacgatcgc gcgtgcttca tccatcacct tttcgaaatt cttgctgcgg tgcgagatct
     1801 cgagaagcga aagattaagg ttgtcgaaat taatgcaggc ttctgcggcc tgtttcagca
     1861 cgtcaacggg aaggatgccc ggccctgcgc tgaaattgtg ggcttttttc gtaaggacag
     1921 tggaagacat agcttggaaa attggtactc aaaggtagaa attagttggg tggaaagacc
     1981 ggaaaataaa cccggaaaaa taaatttggg ttaagcgaaa gcagcgccgt tcttacattt
     2041 tcccgataag cgttcgcagt tcggaaaccg cggttcgcca gatgtcttcc ataatttcat
     2101 ccggcttaaa atacgctccg ccgaaattcc cgtcaccgag gtagtcacgg atctgtgccg
     2161 gtgaaagcga acgcaggtgc gctgtgtcga tcattttctt cggcgttccc ggttgctcca
     2221 cgtcgctgat ccgcgtccac ggaaaatttt ccatccacga ggcatgcgaa gcgacgggat
     2281 cgtattccat tacttttttc cacgtt
//
LOCUS       DASTSF010000360         7827 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1293244,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000360 DASTSF010000000
VERSION     DASTSF010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 7827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7827
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1293244"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="VFU15_10295"
     CDS             <1..307
                     /locus_tag="VFU15_10295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718216.1"
                     /translation="YSEQQNKVLQSMTKPEVDALAKKWLDTSHMVIMVVGDSAKIAPG
                     LAKLGYEVVNVDDNGNAVKPAVKDVKSAGQVNQVPANSGDKKKKKKKSKKDLKLQNQ"
     gene            340..750
                     /locus_tag="VFU15_10300"
     CDS             340..750
                     /locus_tag="VFU15_10300"
                     /inference="COORDINATES: protein motif:HMM:NF039496.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5655 domain-containing protein"
                     /protein_id="HEU4718217.1"
                     /translation="MTGWKCPECGRTFAVKTKEHSCVKIPVGSHFEGKPASLRKAFDK
                     MVAISSSFGNVSVQPVKGGIFLKKAGTFAMIAVRKDCLKVEFFLSHLYEGFPVEKTFR
                     YTQKKIVHVVSISGPGDVNKQFAGWLKESYSLAK"
     gene            856..1455
                     /locus_tag="VFU15_10305"
     CDS             856..1455
                     /locus_tag="VFU15_10305"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU4718218.1"
                     /translation="MSLTAKGNAMQIESKYHVSREQIAAEEAEIEAARTDPQKFAPLY
                     DRYYERILGFVYQRVESRDDACDVTAQVFIAALENIGKYKAQGVPFSAWLFRIAVNEL
                     SRMYRRAKVRQAISIDDTQVEDILHELGEENTSVTDARLMKALQQLGPEEVMLIEMRF
                     FEKRPFKEVCDILAISETAAKARVYRLLERLKTIYTSLA"
     gene            1462..3018
                     /locus_tag="VFU15_10310"
     CDS             1462..3018
                     /locus_tag="VFU15_10310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718219.1"
                     /translation="MDKYTIKRNREPLTPEDVRKGKDFGSFMNAYAVRKKPFYTKNWF
                     LSSLSVAALAAGIITMNIKNAEQPVFPLQSAFISPPLNGIDIADTVFTVKPGQGGNFF
                     SDNGSFITVPANAFLDSAGKTVDGNVELHYRELPDPASAFLAGIPMTYDSAGARYHFR
                     WNGMMEIYAFCNGKPLKADPQHPVLVSLPAEKEQMQYGTYYLDTIQKAWTYKGRNDWN
                     FAALVSDTSGAAGLPVKQDAGNDQLLPPRKANRSRHSFAIKFDPQEFPELAIYKGVRF
                     EVDETKTPYRGSDAKVKWEDAVVTKNRNADTYTITFTRGVLEKSYVAYPVVGETDYAA
                     AKKLYEEKYSEYLQGTRNGAVPHFALSPGARAVFTSDSCFAQALALHNAHSGTNEHAM
                     YMRAFTVSDFGIWMAGVPQNLPQGATTSLSLKDGRTKKELVTNRVYLVEKGTNALFTY
                     DAAGLADFTYDPAQENSIWAVTADGKPAVFYPGDFKTLGTSEKKTGITMRVPAQKISN
                     AAQAMTVLGS"
     gene            3129..3665
                     /locus_tag="VFU15_10315"
     CDS             3129..3665
                     /locus_tag="VFU15_10315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004330020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HEU4718220.1"
                     /translation="MKTGTEEQLPDDLLQKRIYFIRGQKVMIDRDLAALYGVPTKALK
                     QAVKRNLARFPPDFMFVMTAKELENWRSQIVTSNSEKMGLRHKPFCFTEHGVTMLSCV
                     LSSERAIAVNIRIIRVFIRMREAVTDSREMRLKLELLEKEVGKNSADIKSVFAALRQL
                     FDPDRPPRKQIGYKNGER"
     gene            3719..5497
                     /gene="recJ"
                     /locus_tag="VFU15_10320"
     CDS             3719..5497
                     /gene="recJ"
                     /locus_tag="VFU15_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEU4718221.1"
                     /translation="MEMKVSLKMENGTGKYNENGTRNRWSLKKQPAKEELKALARQLN
                     GNEIVASLLLQRGIRTQEEAKRFFRPDLSDLHDPFLMKDMDAAVARVERALLRKEKIL
                     VYGDYDVDGTTAVALVYTHLVSLGADCGFYIPDRYAEGYGISFAGIDFAKENGFTLII
                     ALDCGIKSNDKIAYAKGKNIDFIICDHHRPGAELPGAVAVLDPKRNDCHYPYDELCGC
                     GIGFKLMQALTQKKGGSVEMIEPLLDLVAVAIAADIVPLTGENRVLATFGMKRINSAP
                     RPGFRAMTELVNVKRKLSISDVVFLFAPRINAAGRVEHGKLAVELLASDDEASARRAA
                     EIINRNNTERRELDRQITIHALDMISSDAAFAKRRSTVVFDSTWHKGVVGIVASRLIE
                     KHYRPTIVLCESNGKVSGSARSVKDFDIYEAIEACSDLLIQFGGHKYAAGLTLEKQNV
                     DAFIEKFEAEVSARITEAQLVPEIEIDAEISFSQIDNQLLNTIRLFAPFGPGNMNPVF
                     LTRHVCDRGWARVVKDSHLKLDLIDPRFPTRIFPAIAFGLAEKLPLLANKNEIDAVYT
                     LEENEWNGKVSLQLNVKDVKRFVNGN"
     gene            complement(5562..7553)
                     /gene="dnaG"
                     /locus_tag="VFU15_10325"
     CDS             complement(5562..7553)
                     /gene="dnaG"
                     /locus_tag="VFU15_10325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016194066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEU4718222.1"
                     /translation="MIPKETVDRIFDTARIDEVVGEFVSLKKRGANMIGLCPFHNEKT
                     PSFNVSPSRGIYKCFGCGKGGNSVNFVMEHEHLSYPEALRWLAKKYNIEIEEEEQSAE
                     QIAKDNERESLFIVSAFAQKHFTKNLHETEEGKAIGLSYFLERGFRPEIIEKFQLGYS
                     PDTWRDLTDAAIAAGYKLEYMVKAGLTIQNEEDKEKYFDRFKGRVIFPVHNASGRVIA
                     FGGRTLKTDKKLAKYINSPETDIYHKSNVLYGLFFAKKTIIELDVCYLVEGYTDVISM
                     HQAGIENVVASSGTSLTVEQIRLIRRYTNNITILYDGDPAGIKASFRGIDLILEEGMN
                     VRVLLFPDNDDPDSYSKKVTSEELKEFIAKNTKDFIRFKTELLSQDVAGDPIRKAQLI
                     RDIVESISLIPDAITRSVFVKDCSRILDIEEQTLLNELNKQRRKNFDKKRSGTSHETH
                     TGPSQETGFVPDIAAKPEERTDTDAQTGTEYQEREIVRLLLNYGQQAITADSEDEEGK
                     PVTIEVSLAEFILSEIDNDQITFEHETYGKIVDEFRKHIAAGNVPDLNYFVNYPDQNV
                     SMLAVDLVTIPYQLSDWDRHFIYVQTENLVLRRTATHGVHAIKLRKIELMINERRKQI
                     RELEDRHEFENMMIVLSEIKSLEEAKKHFSALLGRVVIR"
BASE COUNT         1838 a         2011 c         2227 g         1751 t
ORIGIN      
        1 ctattccgag cagcagaaca aagtgctcca gtcgatgacg aaaccggaag tggatgcgct
       61 ggcgaaaaaa tggctcgaca ccagccacat ggtgatcatg gtggtgggcg attccgcaaa
      121 gatcgcaccc ggtcttgcga agctcgggta tgaggtcgtg aacgtggatg ataacgggaa
      181 tgcggttaag cctgccgtga aagacgtgaa aagcgccggc caggtgaacc aggtacctgc
      241 gaattccggg gacaaaaaga aaaagaagaa aaaaagcaaa aaggatctca agctgcagaa
      301 ccagtaaagg cgtttgccgg cagcggaatt tttttcacaa tgaccggatg gaaatgcccg
      361 gaatgcgggc gcacatttgc cgttaaaaca aaggagcatt cctgcgtgaa gatcccggtc
      421 ggcagtcatt tcgaaggaaa gccggcttcg ctgcgcaaag cgttcgacaa gatggtggct
      481 atttcctcgt cgttcgggaa cgtgagcgtg cagccggtca aaggcgggat cttcctgaag
      541 aaggcgggaa cgtttgccat gatcgccgtg cggaaggact gcctgaaggt ggaattcttt
      601 ctcagccatt tgtacgaagg attcccggtc gagaaaacgt tccggtatac gcagaagaag
      661 atcgttcacg tggtgagcat ttccgggccc ggcgatgtga acaaacaatt cgccggctgg
      721 ctgaaagaat cctattcgct ggcgaaatag agcgtccccg gaccggcatc ttcattcttg
      781 tccgattatt tccaacttta cggtcacttt ccggtacgca gggccttatg ctgatgaaaa
      841 cgaaaccagg tggccatgtc ccttaccgca aaaggcaacg ccatgcagat tgaatcgaag
      901 tatcacgtca gccgggagca gatagcggcc gaggaagccg agatcgaggc tgcgcgtacc
      961 gatccgcaga aatttgcacc gctgtatgac cgttactacg agcgcatcct gggatttgtg
     1021 taccagcgcg tggagagcag ggatgatgcc tgcgacgtga ctgcacaggt gttcattgcg
     1081 gcgctggaaa atatcgggaa atacaaagcg cagggcgtgc cgttttccgc ctggctgttc
     1141 cggatcgcgg tgaacgagct cagccgcatg taccgccgtg cgaaagtgcg gcaggcgatc
     1201 agcatcgacg atacgcaggt ggaagatatc ctgcacgagc tgggcgaaga gaacacatca
     1261 gtcaccgatg cacggctcat gaaagcgctg cagcagctcg gacccgaaga agtcatgctg
     1321 atcgaaatgc ggttttttga aaaacgtccg ttcaaagaag tgtgcgacat actcgccatt
     1381 tccgaaacgg cagcgaaggc gcgtgtatac cgtcttctcg aacgcctcaa aacgatttac
     1441 acttcattag cttaaaacgt catggacaag tatacgatca aacgaaaccg tgaaccgctg
     1501 acgccggagg atgtgaggaa aggaaaggat ttcggctctt tcatgaatgc gtatgcggtg
     1561 cggaaaaaac cgttctacac gaagaactgg ttcctctcct cgctttccgt cgcggcgctc
     1621 gccgcaggga tcatcaccat gaacatcaaa aacgcggagc agcctgtttt ccctttacag
     1681 tcagcattca tttcgccccc gctgaacggc atcgatatcg ccgacacggt ttttacggtg
     1741 aaaccggggc agggtggaaa ttttttcagc gataacggtt ctttcatcac ggtacccgcc
     1801 aatgcatttc tcgacagcgc cgggaaaacg gtggacggaa acgtggagct gcattaccgc
     1861 gagcttcctg atcccgcttc cgcctttctc gccggaattc ctatgacgta cgattctgcc
     1921 ggagcacgtt atcatttcag gtggaacgga atgatggaaa tctacgcgtt ctgcaacggg
     1981 aaaccgctga aggcagatcc gcagcacccg gttctcgttt cgcttccggc ggaaaaagag
     2041 caaatgcaat atggaacgta ttacctcgac acgatacaga aagcgtggac gtacaaaggc
     2101 aggaacgact ggaatttcgc ggcgctggtc agtgacacgt caggtgctgc gggtctgccg
     2161 gtgaagcagg atgctggaaa tgaccagttg ctgccgccgc gtaaagcgaa caggagccgt
     2221 cacagcttcg caattaaatt cgatccgcag gaattcccgg agcttgcgat ctacaaaggc
     2281 gtccgcttcg aagtggatga aacgaaaaca ccgtaccggg gcagtgatgc aaaagtgaaa
     2341 tgggaggatg cagtcgtaac aaagaacagg aatgcggata catacaccat tacattcacc
     2401 cgcggtgtac tggaaaaaag ctacgtggct tatcctgttg tcggcgaaac ggattatgcc
     2461 gccgcaaaaa agctgtacga agaaaagtac agcgaatatc tgcaaggtac gaggaacgga
     2521 gcggttccgc atttcgcatt gtcgcccggg gcacgtgcgg ttttcacttc ggattcctgt
     2581 tttgcgcagg cgctcgcgtt gcataacgcg catagcggaa cgaatgaaca tgcgatgtac
     2641 atgcgtgcgt tcaccgtcag cgatttcgga atatggatgg cgggcgttcc gcagaacctg
     2701 ccgcaggggg caacgacttc actttcgctg aaagacggcc ggacaaaaaa ggagctggtg
     2761 acgaaccgtg tttacctcgt ggaaaaagga acgaacgcat tgttcacgta tgatgcggcc
     2821 ggcctcgcgg atttcaccta tgacccggca caggaaaatt ctatctgggc cgttacagcc
     2881 gacggaaagc ccgctgtttt ttatcccggc gatttcaaaa cgctcggcac ctctgaaaaa
     2941 aagacgggga tcacgatgcg cgttccggcg cagaagatct ccaatgccgc gcaggcaatg
     3001 acggttctcg gtagctgagg tccgtttcca cccgcttata tccgggtata aacgtttaaa
     3061 acacgggaaa tgattttccg gtgcacactt ttgcggccgt tttttcaata aaagcgttac
     3121 gacaaaagat gaaaacagga acagaagagc agctgccgga cgacctgctg cagaaaagaa
     3181 tttatttcat ccgcggacaa aaagtgatga tcgatcgcga tctcgcggcg ttgtacggtg
     3241 tgcccaccaa agcgctcaag caggcggtga agaggaacct cgcccgtttc ccgcccgact
     3301 tcatgttcgt gatgacggca aaggagctgg aaaattggag gtcacaaatt gtgacctcca
     3361 attcagagaa aatgggattg cggcataagc cgttctgctt tacggagcac ggggtaacaa
     3421 tgctttcctg cgtcctgagc agcgaacggg cgatcgcggt gaacataagg atcatccgcg
     3481 tgttcatccg catgagggaa gcggtcacag acagcaggga aatgcgcctt aagcttgagt
     3541 tgctggaaaa ggaagtggga aaaaacagcg ccgatataaa atcggttttt gcagccttgc
     3601 gccagctgtt cgatccggac cgcccgccgc ggaaacagat cgggtacaag aacggcgaac
     3661 gctaaggggc gcatgcgttt aaagaaaata gtttgttaat tagtaatacg tttgatctat
     3721 ggaaatgaaa gtgagcctga agatggaaaa tggaacggga aagtataatg agaatgggac
     3781 gcggaaccgg tggtcgctga aaaagcagcc ggcaaaggaa gagctgaaag ctctcgccag
     3841 gcagctgaac ggaaatgaaa tcgtcgcatc gctcctgctg cagcgcggga tacgcacgca
     3901 ggaagaagca aaacggtttt ttcgtcccga cctcagcgac ctgcatgatc cgttcctgat
     3961 gaaggacatg gatgctgctg ttgcccgtgt cgagcgggcg cttctgcgga aagagaaaat
     4021 tctcgtgtac ggtgattacg acgtcgacgg aacgacggct gttgcgctgg tgtacacgca
     4081 tcttgtttcg ctcggcgcgg attgcggttt ttacattccc gaccggtacg cggaaggtta
     4141 cggcatctcg ttcgccggga tcgattttgc aaaggagaat ggctttacgc tcatcatcgc
     4201 gctggactgc ggcatcaagt cgaatgataa aattgcttac gcgaaaggaa aaaacatcga
     4261 cttcatcatt tgcgatcacc accggccggg cgctgaattg ccgggcgcag ttgctgttct
     4321 cgatccgaaa aggaacgatt gccattaccc gtatgatgag ctttgcggat gcgggatagg
     4381 gttcaagctc atgcaggcgc tcacgcagaa aaaaggaggc agcgtggaga tgatcgagcc
     4441 gctgctcgac ctggttgcag tcgccatcgc cgccgatatt gttccgctca cgggcgagaa
     4501 ccgcgtgctg gcgacgttcg ggatgaaacg catcaactcg gctccgcggc cgggtttcag
     4561 ggcgatgacg gagctcgtga acgtgaaacg aaagctttcc atcagcgacg tcgtttttct
     4621 ttttgcgccg cgcatcaatg cggccgggag ggtggagcac ggaaagctgg ccgtggagtt
     4681 gctcgcttcg gacgatgaag cttccgcacg ccgcgccgcg gagatcatca accgcaacaa
     4741 tacggaacgc cgcgagctcg accggcagat caccattcac gcgctcgata tgatctcttc
     4801 cgacgctgca tttgcaaaac ggagatcaac ggtcgtcttc gacagcacct ggcacaaagg
     4861 cgtcgtgggg atcgtcgcgt cgcgcctcat tgaaaagcat taccgcccca cgatcgtgct
     4921 gtgcgagtcg aacggaaaag tttccggctc cgcgcgttcg gtgaaggact tcgatatcta
     4981 cgaagcgatc gaagcgtgca gcgacctgct catccagttc ggcgggcata aatatgcggc
     5041 gggacttacg ctcgagaagc aaaatgtgga tgcgttcatc gaaaagttcg aagcggaagt
     5101 ttcggcgcgc atcacggagg cgcagctcgt gccggaaatt gagatcgatg cggaaatttc
     5161 cttttcgcag atcgacaacc agctgctgaa tacgatacgc ctgtttgcgc cattcggccc
     5221 gggaaacatg aacccggttt tcctcacgcg gcatgtctgc gaccgcgggt gggcgcgcgt
     5281 ggtgaaggat tcacacctca agctcgacct catcgacccg cgttttccca cgcgcatctt
     5341 cccggccatt gcgttcggtc tcgcggaaaa gcttcccctg ctggcgaaca aaaacgagat
     5401 cgacgccgtt tacacattgg aggaaaacga atggaacggg aaagtttcgc tgcagctgaa
     5461 tgtgaaggat gtgaaaaggt ttgtcaatgg aaactgactt cgcgtcttcg tgccggtgcg
     5521 gttaaagacg gcaaaattcc gtgtacccgt cgtgaaaaac ttcaccgtat caccactctt
     5581 cccagcaggg ctgagaaatg tttcttggct tcttcgagcg atttgatctc tgaaagcacg
     5641 atcatcatgt tctcaaactc gtgcctgtcc tccagctcac ggatctgttt cctgcgttca
     5701 ttgatcatca gctcgatctt gcgcagcttt atcgcatgca cgccgtgcgt ggctgtcctg
     5761 cgaaggacca ggttttccgt ctgcacgtaa atgaaatgcc ggtcccagtc ggaaagctgg
     5821 taagggatgg tgacgaggtc caccgcgagc atgctcacgt tctggtcggg gtaattgacg
     5881 aaataattca gatcaggaac gtttcccgct gcgatgtgtt tccggaattc atccacgatc
     5941 tttccatacg tttcatgctc gaacgtgatc tggtcgttgt cgatctcgct gagaataaat
     6001 tccgcgagcg aaacttcgat cgtcaccggt ttcccttcct catcttccga atcagcggtg
     6061 atggcctgct ggccgtaatt gagcagcagg cggacgatct cacgctcctg gtattcggtg
     6121 cccgtctgcg cgtcggtgtc tgtccgctct tcgggctttg cagcgatgtc cggaacaaac
     6181 ccggtttcct gcgatggccc ggtgtgcgtt tcgtgagatg tgccgcttct tttcttatcg
     6241 aaatttttcc ggcgctgctt gttgagctcg ttcagcagcg tctgttcctc gatgtcgaga
     6301 atgcggctgc agtcttttac aaaaacgctt ctcgtgatcg cgtcggggat cagcgagatg
     6361 ctctcgacga tgtcgcggat gagctgtgct ttccggatcg ggtcgccggc gacgtcctgt
     6421 gaaagcagct ccgtcttgaa ccggataaaa tctttcgtgt tcttcgcgat gaactctttc
     6481 agctcttcgc ttgtcacttt tttcgaatac gaatccgggt cgtcgttgtc ggggaacagc
     6541 agcacgcgca cattcattcc ttcctcgagg atgaggtcga tgccgcggaa gctcgctttg
     6601 atgcctgccg gatcgccgtc gtacaggatg gtgatgttgt tcgtgtaacg gcggatcaaa
     6661 cggatctgct ccaccgtcag cgaagttccc gaggaggcga cgacattttc gatgcccgcc
     6721 tggtgcatgg agatcacgtc ggtgtaacct tccacgaggt agcagacgtc cagctcgatg
     6781 atggttttct tcgcgaagaa aagtccgtaa aggacattgc tcttgtggta gatgtccgtt
     6841 tccggcgagt tgatgtattt cgccagcttc ttgtcggtct tcagcgttct tccgccgaag
     6901 gcgatcacgc gcccgctcgc gttgtgcacg ggaaagatca cgcgtccctt gaagcggtcg
     6961 aaatattttt ccttgtcttc ctcgttctgg atcgtgagcc cggctttcac catgtattcg
     7021 agcttgtagc ccgcggcgat agcggcatcc gtgaggtcgc gccacgtatc gggcgaatag
     7081 ccgagctgga atttttcgat gatctcggga cggaaaccgc gctcgaggaa ataggaaagg
     7141 ccgatcgctt ttccttcttc ggtctcgtgc aggtttttcg tgaaatgctt ttgcgcgaat
     7201 gcggaaacga tgaaaagact ttcccgctca ttgtcttttg cgatctgctc ggcgctctgt
     7261 tcctcttcct cgatctcgat gttgtatttt ttcgcgagcc agcgcagcgc ttcgggatag
     7321 gagaggtgct cgtgctccat gacaaaattc acggagttgc cgcctttccc gcagccgaag
     7381 catttgtaga tcccgcgcga aggagaaacg ttgaacgaag gcgtcttctc gttgtggaac
     7441 gggcacaggc cgatcatgtt cgcgccgcgc tttttcagcg aaacgaactc gcccaccact
     7501 tcgtcgatgc gggcggtgtc gaatatgcgg tcgaccgttt ctttggggat cattgcaaaa
     7561 gggcaatgaa attacgaaag gaagcggaaa agaccgaggg aaaaaagttc atgcccgggg
     7621 gctgctttct caacgcgcta acggacaacc gaataatctc cagcgagcag gcggttactt
     7681 atccccgtaa tctttttcct tcgtattgcc gtattcatcc cagaactgcc atttgccgac
     7741 cttgctcccg tgtttgtaat tgccttcctc tgctttttta ccgtcggcgt aataggccac
     7801 gcagtaacct tcacggtaac cctggtg
//
LOCUS       DASTSF010000361         2380 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_87667,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000361 DASTSF010000000
VERSION     DASTSF010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2380
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87667"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..565)
                     /locus_tag="VFU15_10330"
     CDS             complement(<1..565)
                     /locus_tag="VFU15_10330"
                     /inference="COORDINATES: protein motif:HMM:NF015081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEU4718223.1"
                     /translation="MNVIEVKDRNRTLSIAVEDTGDVHSDTEASKPVLFFVHGLALDH
                     VLWKYAIEYLRADFRCIAIDLPGHGASAGQRPEQAGNFTMSFYAGIVRSCIEKLGLKD
                     VTLAGHSMGAQISIITALQIPSLVQRLVLVSPAGIEKFIPEEAAKIEVGTEMLYRAPM
                     DVKHILGMYAPQMNVHAERARELANDHI"
     gene            complement(701..1747)
                     /gene="phaC"
                     /locus_tag="VFU15_10335"
     CDS             complement(701..1747)
                     /gene="phaC"
                     /locus_tag="VFU15_10335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008902711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class III poly(R)-hydroxyalkanoic acid synthase
                     subunit PhaC"
                     /protein_id="HEU4718224.1"
                     /translation="MAGENIAKQLSETGMKLVNGYDNLVRAGEVEVATTPKDLVFKVD
                     KIKLYRYRRDTPAKFTTPLVISYALVNRHDMLDIQPDRSFVKNLLALGLDIYIIDWGY
                     PTKADKDVTLEDYVNWFLDDIVDFVRRNNGVEKVNLMGICQGGTLSLIYSALHPGKIR
                     NLVTLVTPVDFSTNEGLLFRWSKNMNFDRIVDSYGTVPGEFLNNGFAMLKPMMRATKY
                     MGMVDIMQDKDKLMNFLRMEKWINDSPDQAGECFRQFMKDLYQGNKLIKGELEIGGEK
                     VDLKNITMPVLNIYAAEDHLVPPSATIPLNEHLGTKDNELYKFPGGHIGVFVGSRSQK
                     ELAPAVIKFLGDRD"
     gene            complement(1786..>2380)
                     /locus_tag="VFU15_10340"
     CDS             complement(1786..>2380)
                     /locus_tag="VFU15_10340"
                     /inference="COORDINATES: protein motif:HMM:NF021246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="poly(R)-hydroxyalkanoic acid synthase subunit
                     PhaE"
                     /protein_id="HEU4718225.1"
                     /translation="LYPFLAFGNNPDNPYNNIFSVYQRALSPLVKLFNPGKEAEMNEH
                     IVGMGEKMAVYGQKLAELQQHIYATAAQSWETFVLENYEQVKKGADLSNTQEAFQKWI
                     NRNEEVFIGLFRSEEYSKLQGELLDLGLEIRQHAEKAGEIVLQPFPVVLRSEADDLYT
                     TVYELRKRISALEKQQQESETETKEPKSSAKKKTATV"
BASE COUNT          499 a          637 c          645 g          599 t
ORIGIN      
        1 agatatgatc attcgccagc tcacgcgcgc gttctgcatg aacgttcatc tgcggtgcgt
       61 acatgccgag aatgtgcttc acgtccatcg gtgcgcggta cagcatttcc gtccccacct
      121 cgatcttcgc cgcttcttcg ggaatgaatt tttcgatgcc ggccggcgat acgagcacga
      181 gacgctgcac aagcgaaggt atctggagcg cagtgatgat gctgatctgc gcgcccatgg
      241 aatgtcccgc aagggtgacg tctttcagcc cgagcttctc gatgcacgaa cggacgatgc
      301 ccgcgtaaaa tgacatcgtg aaatttccgg cttgctccgg ccgctggccc gccgaggcgc
      361 cgtgaccggg aagatcgatg gcaatgcagc ggaaatcggc gcgaaggtat tctatggcgt
      421 atttccagag aacgtggtcg agcgcaagcc cgtgaacgaa aaacaggacg ggtttcgaag
      481 cttccgtgtc cgaatgaacg tcaccggtat cttcaacggc aatggaaagt gtcctgttcc
      541 tgtcctttac ttcaataaca ttcatgccgg ggaaagttta gcatgtgatc tcaataaaaa
      601 tagaattttt tggctgaaaa acaccacacc ccggtaaaaa ccggggtgca gtgaacaagc
      661 aaacaagatt gcagctggcg gtgctgcaac atcacactct tcagtccctg tcgccgagga
      721 atttgatgac ggcaggcgca agctctttct gcgagcggga accgacgaaa acgccgatgt
      781 gcccgcccgg gaatttatac agttcgttgt ctttcgtgcc gagatgctca ttcagcggga
      841 ttgtcgctga aggcggaacg agatgatctt ccgcggcata aatgttcagc acgggcatgg
      901 taatgttttt caggtccacc ttctcgcctc cgatctccag ttcgcccttg atgagcttgt
      961 tgccctggta aaggtccttc atgaactggc ggaaacattc gcctgcctgg tcgggtgaat
     1021 cgttgatcca cttttccatg cgcaggaaat tcatcagctt gtctttatcc tgcatgatgt
     1081 ccaccatgcc catgtatttc gtggcgcgca tcatcggctt cagcatcgcg aaaccgttgt
     1141 tcaggaattc cccgggaact gtcccgtacg aatccacgat gcgatcgaaa ttcatgttct
     1201 tcgaccagcg gaaaagcagt ccttcgttcg tgctgaaatc gaccggcgta acgagcgtca
     1261 ccaggttcct gatcttcccg ggatgaagtg cagaatagat caggctcagc gttcctccct
     1321 ggcagatgcc catgaggttc actttttcca ccccgttgtt gcggcgcaca aaatcgacga
     1381 tgtcatccag gaaccagttc acataatcct cgagcgtgac gtctttatcc gctttcgtgg
     1441 gatagcccca gtcgatgatg tagatgtcga gtcccagggc cagcaggttc ttcacaaagc
     1501 tgcggtcggg ctggatgtcg agcatgtcat gacggttgac cagcgcgtac gagatcacga
     1561 ggggtgtcgt gaatttcgcg ggtgtatcgc ggcggtaacg gtaaagcttg atcttgtcca
     1621 ctttgaaaac aagatctttc ggcgtggtcg ccacttccac ttcacccgca cgtacgaggt
     1681 tgtcgtaccc gttcaccagc ttcatgccgg tctctgaaag ctgctttgcg atgttttcgc
     1741 ctgccatgtc ggttcggttt tcgttcgttg aaaacaatga ggcggttaaa cggttgccgt
     1801 tttcttcttc gcggaagatt tcggctcttt cgtttccgtt tcagattcct gctgctgctt
     1861 ctcgagcgcg ctgatgcgct tgcggagctc gtaaacggtg gtgtaaagat cgtcagcttc
     1921 gctgcggagc actaccggga aaggctggag aacaatttct ccagctttct cagcatgctg
     1981 gcgtatctcg aggccgagat cgagcagctc gccctggagc ttcgaatatt cctcgctgcg
     2041 gaaaagcccg atgaaaactt cttcgttgcg gttgatccac ttctggaacg cttcctgcgt
     2101 attggacagg tctgcgcctt ttttcacctg ctcgtagttc tcgagcacga atgtttccca
     2161 cgactgtgca gccgttgcgt agatgtgctg ctggagctcg gcgagcttct gaccgtatac
     2221 cgccatcttt tcacccatgc caacgatgtg ctcgttcatt tccgcttctt tgccgggatt
     2281 gaacagcttc acgagcgggg aaagcgcacg ctggtacacg ctgaaaatat tgttgtacgg
     2341 gttatcggga ttgttgccga acgcgaggaa aggataaagg
//
LOCUS       DASTSF010000362         2499 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_680057,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000362 DASTSF010000000
VERSION     DASTSF010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2499
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_680057"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1369)
                     /locus_tag="VFU15_10345"
     CDS             complement(<1..1369)
                     /locus_tag="VFU15_10345"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEU4718226.1"
                     /translation="MPPIREFWSLAPSQLMENLGTGENGLSVEAAEERKAPQKGKNRS
                     QFVSDLLLFLSQFKSPLVLLLVFSLILSSVLGEISNSVIIFVILFLTGILGFFQERNA
                     GRAVEKLRNMVSANATVIRGGDPVLVNAGDVVPGDILLLDAGDIVPGDGLILSSKDLY
                     TNETVLTGESFPAEKTAATVPADAPVSARKNSVFRGTSVVSGTAKVIVAATGKDTELG
                     GIEKEMGVVTAETAFEKGVRNFGYLLMRVSLVLAGTILIVNLVLGRPVAESILFALAI
                     SIGLTPELLPSILTITLSAGARRLAKKKVIVRKLASIQNLGAINVLCSDKTGTLTEGE
                     VKIHSMTDADGKNNPKIGLYALLNAKFESGFTNPMDEAIRVNIKEDVSAYEKLDEVPY
                     DFIRKRLSVVVKHDHERVMITKGALSAVLNVCTQAEREDGTVVPIDEAITAKINGMYR
                     EFSAQG"
     gene            complement(1498..2442)
                     /locus_tag="VFU15_10350"
     CDS             complement(1498..2442)
                     /locus_tag="VFU15_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010665368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HEU4718227.1"
                     /translation="MRSETRFPFVVLLLFSSATMCFAQPSERKNTPQDYIDKYKDDAI
                     REMQLWKVPASITLAQGMLESDNGNSALAVYANNHFGIKCHNDWTGDTYTANDDTANE
                     CFRKYASVYDSYVDHSKFLKGRDRYAFLFDLKITDYKGWANGLKQAGYATDPKYASRL
                     IHIIELYKLYEFDKQNGLPPAGVTVKNPAGANVSTTVTRREIYTNNNVKYVIVREGDS
                     FVKIARELDMGVWQLHKYNELGKDATLKPGMMLYIQPKRRHSIDTDTHTVKPGETMYD
                     IAQQYGMKVSALYKINGMAPGTQPGTGDVLRLKKSRRK"
BASE COUNT          538 a          696 c          661 g          604 t
ORIGIN      
        1 ggccctgcgc gctgaactca cggtacatac cgtttatttt tgcggtgatc gcttcatcaa
       61 tggggacaac cgttccgtcc tccctttccg cctgcgtgca cacattgagc acagccgaca
      121 gcgcgccctt cgtgatcatc acgcgttcat ggtcgtgctt cacgacaacg ctcagccgct
      181 tccggatgaa atcgtacggc acttcatcga gtttttcata cgcggaaaca tcttccttaa
      241 tgttgacacg tatcgcttcg tccatcgggt tggtaaaacc ggattcgaat ttcgcattca
      301 gcagcgcgta cagcccgatc ttcggattgt tttttccatc cgcatccgtc atcgaatgta
      361 tcttcacctc accttcggtg agcgtgccgg ttttatcgga gcagagcaca ttgatcgcgc
      421 cgaggttctg tatcgaagcc agcttccgca cgatgacttt ctttttcgcc aggcgtcttg
      481 caccggcaga gagtgtgatc gtgaggatgg acggaagaag ctccggcgtc agtccgatgg
      541 aaatggcgag cgcgaagaga atggattccg ccaccggccg gccgagcacg aggttcacga
      601 tgaggatcgt tccggccagc acgagtgaaa cgcgcatgag caggtagccg aaattccgca
      661 cgcctttttc aaacgccgtt tcagccgtga caacgcccat ttctttttcg atgccgccga
      721 gctccgtgtc tttcccggtt gcagcgacga tcaccttcgc tgtcccgctg acaacgctcg
      781 ttccgcggaa cactgaattc ttccgggcag aaacaggagc gtcagcagga acggtcgcag
      841 ccgttttttc cgccgggaat gattcgcccg tcagcaccgt ttcgttcgtg tagagatctt
      901 tggaagagag aataagcccg tcacccggaa cgatatcacc cgcatcgaga agaagaatat
      961 cgccgggaac aacgtccccc gcattcacca gcaccgggtc tccgccgcgg atcaccgttg
     1021 cgttcgcact taccatgttg cgcagcttct ctactgcacg tcccgcattt cgttcctgga
     1081 aaaacccgag aatgccggtg agaaaaagga tcacgaaaat gatcaccgag ttgctgatct
     1141 cgcccagcac ggaagaaaga atgagcgaga atacgagcag cagcacaagc gggcttttga
     1201 actgcgaaag aaaaagcagg agatcgctta cgaactgaga acggtttttt cccttttgcg
     1261 gggctttcct ttcttcagct gcttccacgg aaagaccgtt ctctcccgta cccaggtttt
     1321 ccatgagctg cgaaggagca agagaccaga attcacgaat gggcggcatg atttccccgg
     1381 caattgagca ggaagaacga agctaatgtg aacatacagc ccggaggctg acgaacgaca
     1441 ggcaagaaga aaaaactgat cagtttccgg ttgatatttt ccggcgggcg gtgcaggcta
     1501 tttcctgcgg cttttcttca gcctgagaac atcaccggtc ccgggctgcg tacccggcgc
     1561 catgccgtta atcttgtaga gcgcgctcac tttcatgcca tactgctgcg cgatatcgta
     1621 catcgtctcg ccgggcttga ccgtgtgcgt atccgtatcg atggaatgcc tgcgtttggg
     1681 ctggatgtaa agcatcatgc cgggtttcag cgtcgcatcc ttcccgagct cgttgtattt
     1741 atgcagctgc catacgccca tgtcgagctc ccgcgcgatc ttcacgaacg aatctccttc
     1801 gcggacgatc acgtacttca cattattgtt ggtgtaaatt tcccggcgtg tcaccgtagt
     1861 gctgacattc gcgccggccg ggttcttcac cgtcacgccg gcaggcggca aaccgttctg
     1921 cttgtcaaat tcgtacagtt tgtacagctc gataatgtga atgaggcgcg aagcatattt
     1981 gggatcggtt gcatatccgg cctgcttcag cccgttcgcc cagcctttgt aatcggttat
     2041 tttcagatcg aacaggaagg cgtaccggtc gcggcctttc aggaatttcg aatgatcgac
     2101 gtaggaatca tacactgaag catatttccg gaagcattcg ttcgccgtgt cgtcgttcgc
     2161 cgtgtaggtg tcgcctgtcc agtcgttgtg gcacttgatg ccgaaatgat tgttcgcata
     2221 aacagcaagc gcgctgttgc cgttatccga ttcgagcatg ccctgcgcaa gcgtgatgga
     2281 tgcaggaact ttccacagct gcatttcgcg tatcgcgtca tccttgtatt tgtcgatgta
     2341 atcctgcggc gtgttctttc gctcggaagg ttgcgcaaag cacatcgtgg cactgctgaa
     2401 aagcagcagg acgacgaacg ggaaacgggt ttcgctgcgc atgacggaaa atggcttttt
     2461 acaaaattac ccggcgtaaa aagccttccc tggttgctt
//
LOCUS       DASTSF010000363         3635 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1273838,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000363 DASTSF010000000
VERSION     DASTSF010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3635
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1273838"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1772)
                     /locus_tag="VFU15_10355"
     CDS             complement(<1..1772)
                     /locus_tag="VFU15_10355"
                     /inference="COORDINATES: protein motif:HMM:NF013125.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HEU4718228.1"
                     /translation="MKKNSHTNGSGALFRRMLLIFAFGSGSLAAQTFTESMGSVASTT
                     SIVSYEAANGFDNDGFTMTGNADIRNTSASNGYAGASGLANVFFTNNTTNPAGNRSFQ
                     IEGINTSNISDPVLSFGILKSTVASDGSDFIVEVSTDGINYTPVSFPALPTGTGTAVW
                     RYETVTGAIPQVSDLRIRWTNTSATTQYRIDDVNLNCSASITPSQSTTLCAGDNVTLT
                     ASNALGGSYLWSNSAATQSISAGSSATYTVQVTDGSGCVSVSSPVRVLAYPAAMVSAS
                     ASDSTICTGDSATLHARTLTQDLIFSEYVEGSGNEKYIEIYNGTGAAVNLSDYEYLAF
                     HNGAASPSFTTALSGTLADGATLVLKNGSATLYSGGQAEFGVQHNGNDALALYKISTS
                     SYVDIFGVIGQDPGTAWTGPGGYSTMDHTLRRKPNVYSGITVNPGLAGANGFTTLTTE
                     WDLYATDDVSGLGMHSVDGAYVWNPGSYSGNMVSVSPSSTTTYTVTGTFINGCTASDD
                     VTINVSNPSVSVSASDTLIYLCYGPQSSTLTANASGGTAPYSISWSTGETTSSVTVSP
                     NTTTTYSVTVSDNNGCTSSSSSIAV"
     gene            complement(2127..>3635)
                     /locus_tag="VFU15_10360"
     CDS             complement(2127..>3635)
                     /locus_tag="VFU15_10360"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HEU4718229.1"
                     /translation="STNVTQPPAITISTSSVDAHCNQIDGSVSASASGGTGTLNYQWN
                     GGPASQNYNNIPAGNYSVVITDQNGCSDSSSVVVNNLNGVTASAGTVTNVTCSGQSDG
                     SIVINANGGNPAYTYSWSPNVSATDTANGIPAGNYQVTVSDAAGCTSLVSVTVTEPPQ
                     LSVTAAASPSAVCEGSPLQLSSSANGGTPGYSFTWMPGNMSGANQNTVPASTTTYSVS
                     VTDQNGCTATGSVNVIVSAMPVAAFAADTLSGCAPLCVNFSDLSTVTAPSAITSWNWD
                     FGDGNISTQQNPSHCYDTPGIYTVVLKVTTAAGCETTITMTNYINAFVTPVAGFTYSP
                     QPVSILDPEVFFTDTSWNAVTWSWSFGDLNNSSSASQNPSFVYPDATCYQVLLSVTSA
                     NGCTDTATQDICINPDVTLYIPNTFTPDGNGMNDVFLPAGIGLTNDNYDLMIFDRWGN
                     LIFESHSLDQGWDGRANGGSEIAQIDTYVWKLHVTDILGKEHSLIGHVNLIK"
BASE COUNT          854 a          875 c         1091 g          815 t
ORIGIN      
        1 actgcaatcg acgaagaaga cgaagtgcat ccgttgttat cgctaacggt tacggaataa
       61 gtggtcgtcg tattcggtga aacagttacg gaagaagttg tttcgcccgt gctccacgag
      121 atagaatacg gggctgttcc gccggaagcg ttcgcagtga gcgtggatga ctgcgggccg
      181 tagcagaggt aaataagcgt atcgcttgcc gaaacggaaa cggacgggtt gctgacgttg
      241 atcgttacgt cgtcactggc cgtgcaaccg ttgatgaacg taccggtgac ggtgtacgtt
      301 gtggtggaag acggagaaac agagaccatg tttccgctgt aacttccggg attccataca
      361 tacgctccgt cgacgctgtg cattccgagt ccggagacgt cgtccgttgc ataaagatcc
      421 cattcggtgg taagcgtggt gaaaccgttt gcgccggcca gaccgggatt taccgtgatg
      481 ccggaataca cattcggttt gcggcgcagc gtgtgatcca ttgtggaata accgccggga
      541 cctgtccatg ctgttcccgg atcctggccg atgacaccga aaatatcaac gtaagaagaa
      601 gtggagattt tgtacaacgc cagtgcatca ttgccgttat gctgaacacc gaattcagcc
      661 tgcccgccgg aataaagcgt ggccgacccg ttcttcagaa cgagcgtagc tccgtcagcg
      721 agtgtgcccg aaagagcggt cgtgaatgaa ggtgaagctg caccgttgtg gaacgcgagg
      781 tattcgtaat ccgaaaggtt taccgctgcg cccgtaccgt tgtatatctc gatgtatttt
      841 tcattgccgg aaccttccac gtattcggaa aaaataaggt cctgcgtaag cgtgcgcgca
      901 tgcagtgttg cagaatcacc ggtgcagatc gtgctgtcgg aagcagaggc ggaaaccatt
      961 gccgcgggat acgcgagcac acgtacgggt gaagaaaccg aaacgcatcc gctgccgtcc
     1021 gttacctgta cggtgtaggt tgcggaggaa cctgcagaga tcgactgcgt ggcagcgctg
     1081 ttcgaccaga gatagcttcc acccagcgca ttgctggcgg tgagcgtaac gttgtcgccg
     1141 gcgcaaagtg ttgtcgactg cgagggtgtg atggaagccg agcagttgag gttgacgtcg
     1201 tcgatgcgat actgcgtggt cgcgctggtg ttggtccagc ggatacgcag gtcggaaacc
     1261 tgggggatcg cgcccgtcac cgtttcataa cgccagaccg cggtcccggt tccggtggga
     1321 agcgcaggga acgaaaccgg cgtgtaattg atgccgtcgg tgcttacttc aacgatgaaa
     1381 tcggaaccat cggaagcaac ggtgcttttc agtattccga aagaaagcac gggatcggaa
     1441 atgttgctgg tgttgatgcc ttcgatctgg aaggaacggt ttcctgccgg gttcgtcgtg
     1501 ttgttcgtga agaaaacgtt ggccagcccg gatgcaccgg cgtaaccgtt cgacgcggat
     1561 gtattgcgga tatcggcgtt acccgtcatg gtgaacccgt cattgtcgaa accgttggca
     1621 gcttcatagg atacaatgct ggtagtggag gcgactgagc ccatggactc agtgaaagtc
     1681 tgggctgcaa gcgagcccga cccgaaagcg aaaatcaaaa gcatgcggcg gaaaagagct
     1741 ccgctgccat tcgtgtgcga attttttttc attttcatct ggttttagtg aaataaatgt
     1801 gtgtttaatt acaaggctaa agtagaccag tgaagaaaag agagtggtgt ttgataatta
     1861 tacttaagta aaatgcagcc gtgttaaaaa gtaagtggat tttacaggga atgcttacta
     1921 tggaagtact taaatagcat gataagtata atcatgttta cttcggacgg aaatgtcaat
     1981 tcgctattaa gcgccggtgg gcgccggtgt taccaatgtt tagttaacag ggagaaatgt
     2041 tttacagaag aggcggatgg aatttacacc cggctgccgg gccgaagaac gggaggtaaa
     2101 acaaatctga aaagcgggaa cgcgtgttac ttgatcaggt tcacgtgacc gatgaggctg
     2161 tgctctttgc cgaggatgtc ggtcacatgc agcttccaca cgtaggtgtc gatctgcgcg
     2221 atctcgctgc cgccgttcgc tcttccgtcc cagccctggt caagcgagtg ggattcgaag
     2281 atgagatttc cccagcgatc gaagatcatc aggtcgtaat tgtcattcgt gagaccgata
     2341 cctgcgggaa ggaacacatc gttcatgccg ttcccgtccg gtgtgaatgt gttcggaatg
     2401 tagagcgtga cgtcgggatt gatgcagata tcctgcgtcg ccgtatcggt gcagccgttc
     2461 gcgctggtta cggaaagcag tacctggtag caggtggcat cgggataaac gaaagaagga
     2521 ttttgcgagg ccgatgaaga attgttcagg tcaccgaagc tccagctcca ggtcaccgca
     2581 ttccaggacg tatccgtgaa gaaaacttcc ggatcgagaa tggaaacagg ctggggagaa
     2641 taggtaaacc cggcgaccgg tgtaacaaaa gcgttgatgt aattcgtcat cgtgatcgtc
     2701 gtttcgcatc cggcagcggt ggtcactttc agcacgacgg tgtagatccc gggcgtgtca
     2761 tagcaatgcg aaggattttg ctgcgtggaa atatttccgt cgccgaaatc ccagttccac
     2821 gaagtgatcg cggacggggc ggtaacggtg gaaagatcgg agaagttcac gcacaagggc
     2881 gcgcaaccgg aaagcgtatc ggcagcaaac gcagcaacag gcatcgcgct cacaatgacg
     2941 ttcaccgaac ccgtcgccgt gcatccattc tggtcggtta cgctaacgct gtatgtcgtc
     3001 gttgaagccg gaaccgtatt ctggttagcg ccgctcatgt tcccgggcat ccacgtgaac
     3061 gaatagcccg gcgttcctcc gttcgcagag gaagaaagct gcagcggaga tccttcacac
     3121 accgcggaag gtgaagctgc ggccgtaacg gaaagctgcg gtggttcagt aaccgtaacc
     3181 gaaacgagag aggtgcagcc cgccgcatcg gaaactgtca cctggtaatt ccctgcggga
     3241 attccgttcg ctgtatccgt tgcgctcaca ttcggcgacc agctgtaggt gtaagcggga
     3301 ttgcccccgt tcgcattgat gacaatggaa ccgtccgatt gaccggagca cgtcacgttc
     3361 gtcactgttc ctgcggaagc cgtcacaccg ttgaggttgt tcacgaccac ggaagaagaa
     3421 tcgctgcacc cgttctggtc ggtgatgacg accgaataat tcccggcggg gatgttgtta
     3481 tagttctgcg aagcaggacc gccgttccac tggtaattca gagtgcccgt tcctccggat
     3541 gcgctggcag aaacagatcc atcaatttga ttgcaatgcg catcgacgga agatgtgctg
     3601 atcgttatcg ccggcggttg cgtcacgttc gttga
//
LOCUS       DASTSF010000364         2382 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1200075,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000364 DASTSF010000000
VERSION     DASTSF010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2382
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1200075"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1182
                     /locus_tag="VFU15_10365"
     CDS             <1..1182
                     /locus_tag="VFU15_10365"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HEU4718230.1"
                     /translation="SATYAVTVNPAPTASVTGTNSICAGGSTTLVATGGGTYAWSSGG
                     TSANETVSPSSTTTYSVIVTNASACSDTATITVTVNPLPNATITGGGNICSGQSITLS
                     TPGTGNYSWSTGSTSSSISVSPSTTTTYSLTVTDPVSGCSSGSSATVTVLAPPVASIT
                     GISSVCAGGIVSLTASGGSSYAWSTGANSASMSDAPVAGTTYTVVVSNGSCTDTATFA
                     VTVNPLPVISTIPDTTINYGQTITIGATSSPGSTLSWSPSSGLSCTTCASPDATPSAT
                     TTYCVLATDGNGCADTACVTIFVDINCGEVFVPNAFSPNNDSKNDVLLVMGNCVTGIN
                     FQVFDRWGEKVFESNDPSMGWDGTYNGKPLDAGVYVYQLEATLVTGDVVQKKGNISLI
                     R"
     gene            1188..2228
                     /locus_tag="VFU15_10370"
     CDS             1188..2228
                     /locus_tag="VFU15_10370"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HEU4718231.1"
                     /translation="MKKFFPFAVLLLAISAKAQDIHFSQFYMSPLTLNPALTGAQHDM
                     QVNLNYKDQWQSVTNPFRTSAVSFDMRTGKNKKSKGFLAFGANIFNDNAGDGKIATTQ
                     GNLSLAYHARIDDNNTFGGGLQAGFAQRSMNGGSLQWGTQFNGFNYDSSLPAQEPSGA
                     SSSFSYADFGAGLDWNHDNISGARNVTNNHDEKFNFGVSVFHLNRPAYSFYGTGEKLY
                     MRFVAHGSAILSLPNSNVAFVPGFMYARQGPQQEIYAGTLIRYVLGMDSKYTGFKQGA
                     ALSIGGFLRARDAIAVVTMLEFSHYSVGFSYDVNVSPLRTASSSRGGFEIALRFVSPN
                     PFQPSGSSRMID"
     gene            complement(2232..>2382)
                     /locus_tag="VFU15_10375"
     CDS             complement(2232..>2382)
                     /locus_tag="VFU15_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018343662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEU4718232.1"
                     /translation="PGYHSNKKHWNTITADGSVPDKLLREWIDHSYELVVNALPKKLR
                     AELDE"
BASE COUNT          525 a          723 c          634 g          500 t
ORIGIN      
        1 tcggcaacct acgcggtaac agtgaatccc gcgcccactg cttcggtaac aggaacaaat
       61 tccatctgcg caggaggatc gacaacactt gtcgcaaccg gcggcggaac ttatgcgtgg
      121 agctcaggcg gaacgtccgc caatgaaacc gtttcaccgt catccaccac cacgtacagc
      181 gtgatcgtga ccaacgcatc cgcctgttcc gacacggcga cgatcaccgt tactgtcaat
      241 ccgctgccga acgcgacaat caccggcgga ggaaatatct gcagcggcca gagcattaca
      301 ctgagcactc cgggaacagg caattattcc tggagcaccg ggagcacttc gtcatcgatc
      361 tccgtttcgc cgtcaacaac gacgacgtat tcactcaccg tcaccgatcc tgtttccgga
      421 tgttcttccg gctcatccgc aacagtgact gtgctcgcac cacccgtggc atcaataacc
      481 ggaatttctt ccgtatgcgc gggagggatc gtctcgctca ccgcttccgg cggatcgtcg
      541 tacgcgtgga gcacaggcgc gaattccgct tcaatgagcg atgcgcctgt tgccggcaca
      601 acgtacacgg ttgtcgtttc caacggctcc tgcacggaca cagcaacatt cgccgttacg
      661 gtgaacccgc ttccggtgat cagcacaatc cccgacacga ccatcaatta cggacagacg
      721 atcacgatcg gcgcgacctc ttccccgggc tcgacgctgt cgtggtcacc gtcttccggg
      781 ctgagctgca ccacgtgcgc ctcaccggac gcaacgcctt ccgcaacgac gacgtattgc
      841 gtgctggcga ctgacggaaa cgggtgcgca gacacagcgt gcgtgacgat cttcgttgac
      901 atcaactgcg gggaagtctt tgtgccgaac gcgttttcac cgaacaatga ttcgaagaac
      961 gacgtcctgc tcgtcatggg caattgcgtg acgggcatca acttccaggt attcgaccgc
     1021 tggggcgaaa aagtattcga gtcgaatgat ccgtccatgg gctgggacgg aacgtacaac
     1081 ggtaaaccgc ttgatgcagg cgtttacgtg taccagctcg aagcgacgct cgtcactggc
     1141 gatgtcgttc agaaaaaagg aaacatttca cttatccggt aagagacatg aaaaagtttt
     1201 ttccgtttgc cgttcttctt ctcgccattt ccgcgaaagc acaggacatc catttctcgc
     1261 aattctacat gtcgccgctc acgctgaacc cggcactgac cggcgcgcag cacgacatgc
     1321 aggtcaacct gaattacaaa gaccagtggc agagcgtgac gaatccattc cgcacatcgg
     1381 cggtctcttt cgacatgcgc accgggaaga ataaaaagtc gaaaggattt ctcgctttcg
     1441 gcgcgaacat cttcaacgat aacgccggcg acggaaagat cgccaccacg cagggaaacc
     1501 tttcgcttgc gtaccacgcc cgcatcgacg acaacaatac gttcggcggc ggactgcagg
     1561 cgggattcgc gcagcgcagc atgaacggcg gaagcctgca gtggggcaca cagttcaacg
     1621 ggttcaatta cgattcttcg ctgccggcgc aggaaccatc gggcgcatcc tccagctttt
     1681 cctatgcgga tttcggcgcg ggcctcgact ggaaccatga taatatttcc ggcgcacgca
     1741 acgtgacgaa caaccacgat gaaaaattca atttcggcgt gagcgtattt cacctcaacc
     1801 gcccggcgta ttcattttac ggaaccggtg aaaagctcta catgcgtttt gtggcgcacg
     1861 gctccgccat cctcagtctt ccgaattcga acgtcgcgtt cgtccccggc ttcatgtatg
     1921 cgcgccaggg tccgcagcag gagatctatg caggcacgct tatccgctac gtgctgggaa
     1981 tggattccaa gtacacgggg ttcaagcagg gagctgcgct ttccatcggc ggtttcctgc
     2041 gcgcacgcga tgcgatcgcc gtggtgacga tgctcgaatt ctcgcattat tccgtgggat
     2101 tcagctacga cgtgaacgtt tccccgctgc gcacggccag cagctcacgc ggaggattcg
     2161 agatcgcgct gcgtttcgtt tcaccgaacc cgttccagcc ttccggctct tcgcggatga
     2221 tcgactgaat tttattcgtc cagctcggca cgcagctttt tcggaagcgc gttcacaacg
     2281 agctcgtacg aatgatcgat ccactcgcgc agcaatttgt cggggacaga accgtcggcg
     2341 gtaatggtat tccagtgctt cttattggaa tgataacccg gc
//
LOCUS       DASTSF010000365        16081 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1791110,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000365 DASTSF010000000
VERSION     DASTSF010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 16081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16081
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1791110"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..310)
                     /locus_tag="VFU15_10380"
     CDS             complement(<1..310)
                     /locus_tag="VFU15_10380"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009055020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEU4718233.1"
                     /translation="MVLILSSSFSANFKPENKKVKVICIDAGHGGKDPGCHGAVAKEK
                     DIALAIALKFGKYIEDNYPDVKVVYTRKTDAFVELNERAAIANRNDADLFVCIHCNSA
                     C"
     gene            555..2984
                     /locus_tag="VFU15_10385"
     CDS             555..2984
                     /locus_tag="VFU15_10385"
                     /inference="COORDINATES: protein motif:HMM:NF040057.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative LPS assembly protein LptD"
                     /protein_id="HEU4718234.1"
                     /translation="MKAGPPGKKESTSQLLGQVTYHAKDSMLFDLPAQKVYLWDSATV
                     DYQDTHLSAYFVTIDFKKNIVIAEGKKDSTGNYITKANFSDNGEAFEANKLSYNYVTG
                     KGKIIDVITEQGDGFLHAQVAKKDTSGTIYVYKGYYTTCDLEHPHFAIRARKLKVIQD
                     DKIITGPAYLEVSDVPTPIAVPFGFFPNKKGRASGILIPTYGESPTLGFFLKDGGYYW
                     GKSDLFDLALRGDIYSKGSWGAKLYTNYKVRYKYSGNFSLKYSRILQGDRELPNRRIA
                     NDFFITWSHTQDPKSNPSIRFSANVRGGTSTYNSYNSTIPTDYLSNTFNSNISWSKSW
                     KFGALSANLRHDQNSLTHNVSMTLPQIAFTVNRFYPFRNPKRVKQKWYDKVGMSYTAD
                     FQNLLSIGDSSLSLRNADYITQHFRNGIHQSLPISTSFNVLRHFTLTYSLTLNSITQF
                     NYVNKRWDGEQVVTDTINGAKVSLDGSTSLTLSTRLFGMYKLKQGRYTVIRHTLTPNL
                     SFSYMPDFTDPRFGFYKTVQTNSVGASSKYSIFENGIYGATPAGRIGSIGLNLINSLE
                     AKLRPDESDSSKTEKRTTLIDAFNVAGSYNLIADHFNWSMLSAGFRTKLFHKVDVNTS
                     FLADPYRVSSEGVRIERFEWREGKRLVRLTGATVSLNTSLRKGGFTSSTTHTSNRGTQ
                     QELDMINANPNAYVDFNIPWSLNFGYSLNWSKPLLTQTVTQTLHVSGDVNVTPKWKVG
                     FDSNYDIKKQEFGFTSLNIYRDLHCWEMQFNWIPFGPRQSYNITINVKSSVLQDLRLT
                     RKRDWYDFTQQ"
     gene            3097..4509
                     /locus_tag="VFU15_10390"
     CDS             3097..4509
                     /locus_tag="VFU15_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012780029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEU4718235.1"
                     /translation="MRKFFFSAAAVTCVAFIFSFTDNAPVKVNPGDNDSTVVAKIYNE
                     ALANGQAYDVLSGLCQKAPKRLSGSPGAAVAVNYMKGVMDSYGFDKVWLQPCTVPHWV
                     RGNPESGKLIFMTGTKKTQHTVNVCALGGSQPTPDKGLQAEVVEVTTFAQLDSLGEAG
                     VKGKFVFYNHKFNPTTISTFSAYGEDVQYRWQGATSAEKYGAVGVIVRSVTASIDTCP
                     HTGAERSDSLAGKIPACAISTVDADYLDDQIRHGVRPEFFLKMNCQWLPDEHSFNVIG
                     ELLGSEHPEQVIVVGGHLDAWDLGTGAQDDGAGCVQSIEAVRIFKTLGIRPKHTIRCV
                     CFMNEENGGKGGKAYADSAAIKKEKHIAAIESDAGGFSPRGFGLSMSQDQRREIEGWK
                     PLLLPYGIYDFSGEGGGSDIDPLQKQGVPMIGLWPDNARYFDYHHTEIDIMKNVNKRE
                     LLMGAAAMSSLIYLIDKYGL"
     gene            4614..4931
                     /gene="trxA"
                     /locus_tag="VFU15_10395"
     CDS             4614..4931
                     /gene="trxA"
                     /locus_tag="VFU15_10395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008243096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEU4718236.1"
                     /translation="MALQITDANFEELVLKSDKPVIVDFWAEWCGPCRMVGPIVEEIG
                     NDYQGRAVVGKMDVDANANIPMQFGIRNIPTILFFKGGKLVDKQVGAVPKAALVQKLE
                     AQL"
     gene            5098..5415
                     /gene="trxA"
                     /locus_tag="VFU15_10400"
     CDS             5098..5415
                     /gene="trxA"
                     /locus_tag="VFU15_10400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012781033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEU4718237.1"
                     /translation="MTAEITDQNFDELVIRPGKTVVLDLWAEWCPPCLKLGPIMEEIA
                     KDFEGKALIGKVNVDRNPEISVQFGIRNIPAILYFRNGKLVDKQIGFVPKETLVQKLA
                     AQL"
     gene            5492..6421
                     /locus_tag="VFU15_10405"
     CDS             5492..6421
                     /locus_tag="VFU15_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014678885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEU4718238.1"
                     /translation="MELNHQELQQFSHLELLARQVVEGFITGLHKSPFHGFSVEFAEH
                     RLYNSGEPTKHIDWKLYGRTDKMFVKRYEEETNLRCQLIIDNSSSMHFPLPEKGKETD
                     NKITFSVKCAAALIELLRMQRDAVGLTVFDEKVSVHTPARSSTMHHKMLFSELEKLLT
                     PEKPEEHRRRTFAIEALHEVAESIHRRSLVIIFSDMFDSRESYDDLFSALQHLKHNRH
                     EVILFHVTDKKHELDFDYDNRPHTFVDMETGEEVKVHPAEVKSVYLKSINDFRHELKL
                     RCGQYRIDFVEADVNEGFRQVLLPYLLKRTRMM"
     gene            6540..8519
                     /locus_tag="VFU15_10410"
     CDS             6540..8519
                     /locus_tag="VFU15_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HEU4718239.1"
                     /translation="MSRDDHQHLHSKVTFAGLLVTLGIIFGDIGTSPLYVLKSIIETP
                     DVHGRVLPINPEIVLGGLSCIVWTLTLQTTIKYVLITLRADNNGEGGIFALYTLVRRT
                     KKKWLLFPAIVGGAMLLADGIITPPISVSSAIEGLSVFNQNINTVPIVIGIIAFLFVI
                     QQFGTKFVGRSFGPVMFVWFSMLAVIGTWMILKCPSVLWALSPHYAVMLIAKHPGSLS
                     LLGAVFLCTTGAEALYSDLGHCGRRNVRVSWTFVKTALLLNYFGQGAWLIMNQGHMLS
                     EFKGDNPFYSMMPPWFYGTGIVIATAAAIIASQALISGSFTLINEAIRLNFWPKVRIK
                     YPTELKGQLYIPSVNWLLMIGCIAVVLHFKRAGEMEAAYGLAIIITMLMTTLLLNYYL
                     VLRRTPRILIFLLLFVYLSIESLFLVANSVKFIHGGYITLMIASLLILVMWIWYDARK
                     IRNRYVEFVHLDQYLPLISELGHDQSIPKYATHLVYLTSADNKDEIESKIIYSILQKR
                     PKRADVYWFVHVHVTDQPYTTEYKVTAMHKKDIFRIDFYLGFRVPTRINLMFRNVVQE
                     MVHNHEVDITSRYESLNRKNVIGDFRFVVLEKYLSNENDLPAYEKVILDIYYLMKPIT
                     LSEESAFGLDTSSVSVEKVPIVIAPQKELHLKRLD"
     gene            8550..9095
                     /locus_tag="VFU15_10415"
     CDS             8550..9095
                     /locus_tag="VFU15_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012927609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU4718240.1"
                     /translation="MTRKTILPPENELVAMLRSKNEQAIRVLYDHYSAALFGVVLRIV
                     NDRETAEDLLQEVFVRIWNNAASYDVSRGRLYTWMLNIARNVSIDKLRSRDFRNHGQV
                     RSLEDYVNTMENHQKEMNVDVIGLKKLVGKLKPEQQQLVDLLYFNGYTQTEAAEKLGI
                     PLGTVKTRIKSALAALREIMQ"
     gene            9092..9925
                     /locus_tag="VFU15_10420"
     CDS             9092..9925
                     /locus_tag="VFU15_10420"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HEU4718241.1"
                     /translation="MTPQELIGSGILEAYATGCATREEITLAEEMLASSPEVRAELAA
                     IRETLEKYASLHAAEPPAGTEEKILAAVGVSRPAPKGKVITLEARLFRRLRNFAAAAS
                     LFVIFSAVFSLLMYFEKSNKAEENDALVAQLNRLQSDYDSTSNMLNTAIADRDQVKHE
                     MSMLRDPMTETITLKSMMKEKPSIKAMVHWNKNDMMVAVDPMTLPPTPSGMQYELWAI
                     VDNKPQSVDVFSVKENPGISMLIPVQKADAFAITLEKEGGTTVPTGQMYVMGHAGSTN
                     P"
     gene            9958..10959
                     /locus_tag="VFU15_10425"
     CDS             9958..10959
                     /locus_tag="VFU15_10425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718242.1"
                     /translation="MKRTIFILLLFPLLFGLGRCKNDVNLLTDYKNYLVCYGLLDPND
                     TVHYIRLSKVFLGEGSAYDMASNPDTANFRPGTADVFVERWLNGVMITSWRLTPDTTI
                     ARDTGIFSNPYQVLYRGNFPVLKDGSIYKLYAKDVARGDIAQSETKIVQDPVITYPAG
                     NFTPLNLYDTTTIRFGIKTGTNGVRYNLFVRFHYKEQFVFDTTEVSDKYIDWQIGTKD
                     ASDDLGGEAIDFTIRRNNFLSAVAALIPVNPDVRRIAGTLDFYYTGASSDLKTYMDVG
                     FANSNSTSPIATFTNITGGLGLFASRTTSIFPNYSIDANTKSALRNNTETVQLGFIR"
     gene            complement(10964..13342)
                     /locus_tag="VFU15_10430"
     CDS             complement(10964..13342)
                     /locus_tag="VFU15_10430"
                     /inference="COORDINATES: protein motif:HMM:NF039201.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEU4718243.1"
                     /translation="MVIFKRLLVAVAVCAGCTQLFSQNANPSLWIPNGPVSAVALKDS
                     TLFVGGDFSMVSPVTGCLVPFDTANASAVQPYPLVYGRVNCICRDSAGYVYVGGKFSR
                     VGNVSASNLFRFTPSGQVDLSFMPNPNGEVFCIENFLKCIFIGGDFTNIFGQTRGRGA
                     RIDSLGELTDFDPRADNTIYAFWPENSNVIAGGQFTAIGGYNIPFLGMLDTATGSSAF
                     ANNLPWQCSQLPNGPVRTIRTFASNIIIGGDFTALGSLNLSGLAIVKSNGVVNTSLIP
                     LVSGKVRTMEFVNGKIFIGGTFTSVRGNPRQNLACIDTSMSLYAWNPSANGTVNCVRT
                     ADGVNFCIGGNFSMLGTDSVYHAGFIDSTGTGTIKQWNAKIDGPVNAMLPGFSGNVLV
                     GGEYSGAGGVDRENLCAIPLSTLRPDSWHPLVASAISAMTVDHSDLYIAGNFAVVDQQ
                     ARNGIASYDLATRTVTTFNPGVSGNVRTMDSDDTTLFIGGNFTQAGGQQRNNIAAIKI
                     ASQSATPWNPNCQGTVNRLIANGNYVYVAGFYSSIGAYPRKNLSRLSKSTGVADGSWS
                     ADTDDGIYDMEIAGGNVYFGGWFSDVAGQPRNHVAAVDTLDGTVQAFNPGSDAYVHTM
                     THYGSDIFFSGMFTVVNNSTFPHLCDYDYNNQQFDTWNPAPDVMPVTMAANQDWLFTG
                     GGFETVSGIYHPNLSMIAITYVTGVPYFRDENSSIRLWPDPSDDIVNFDLPSPNGTST
                     VSIFNATGQLVYREKQSGKMATIPVSGFAPGFYSVVIEGENGEVSRTKFIRE"
     gene            13433..14464
                     /locus_tag="VFU15_10435"
     CDS             13433..14464
                     /locus_tag="VFU15_10435"
                     /inference="COORDINATES: protein motif:HMM:NF025420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4249 family protein"
                     /protein_id="HEU4718244.1"
                     /translation="MKKFFLFASLALFLSTCTTDVDLLDNWKETTVIYGLLNQADTMQ
                     YIRIQKAFLGPDNAYTMAQEFDSINYINQLDVRLEELYNGNVINSWPLYPDTISNKAP
                     GIFSYPMQVVYALHTGAPSFFNTAHQYHLVVKNNQTGNTADATTSLMSDFNMIAPQGP
                     SISIVKINSNTMNSISWGSCPKARIFQVGLTFHYSETDVNNNTVSKATPEWMLGTVYT
                     DSLSALDPQSTTFDPNDFYRFIANNIPQDPNVVSRKDEFITITVYAGGEALYTYMETN
                     GASNSVVQDRPFYTNINNGLGIFSARYGKSKTNLTLTPLTIDTLAMGQFTCGLRFKDR
                     NGVISAGCQ"
     gene            14646..>16081
                     /gene="dnaK"
                     /locus_tag="VFU15_10440"
     CDS             14646..>16081
                     /gene="dnaK"
                     /locus_tag="VFU15_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013633053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEU4718245.1"
                     /translation="MGKIIGIDLGTTNSCVAVMEGNEPVVIPNSEGRRTTPSIVAFVE
                     NGERKVGDPAKRQAITNPRKTIYSIKRFMGHLYDEVTEEIKRVPYSVVKGDNNTPRVQ
                     IDDRKYTPQEISAMILQKMKKTAEDFLGTEVTEAVITVPAYFNDAQRQATKEAGEIAG
                     LKVRRIINEPTAAALAYGLDKKHSDMKIVVFDCGGGTHDVSVLELGDGVFEVKSTDGD
                     THLGGDDFDQKIIEWLVAEFKAENGNLDLTKDPMALQRLKEAAEKAKIELSSSTSTEI
                     NLPYIMPVDGIPKHLVRTLSRAKFEQLVDDLIRRTIAPCKSALQNAGLTVNDINEIIL
                     VGGSTRIPAIVDAVQKFFGKAPSKGVNPDEVVAVGAAIQGGVLTGEVKDVLLLDVTPL
                     SLGIETYGGVCTKLIESNTTIPTKKSETFSTASDNQPSVEIHILQGERPMAKDNRTLG
                     RFILDGIPSAPRGVPQIEVTFDIDANGIL"
BASE COUNT         3973 a         4519 c         4094 g         3495 t
ORIGIN      
        1 cgcaggcgga attgcagtga atgcagacga aaagatccgc gtcgttccgg ttggcgatag
       61 ccgcgcgttc attgagctcc acgaaggcat cggtcttgcg ggtatacacc actttcacat
      121 ctggataatt gtcctcgatg tatttaccaa actttaacgc gatcgcaagt gcaatatctt
      181 tttcctttgc aacagcacca tggcagccgg gatccttgcc cccgtgtcct gcatcgatgc
      241 agatcacttt caccttcttg ttttccggct tgaaatttgc ggagaacgac gaagaaagaa
      301 tcaaaaccat tcctgaaaga aaaaccgcaa tggccagtgt aagcgggaat cgtcgcatca
      361 gtaccataac gcacaaaggg gctttttatt tagttttggt gccgttcagc cgttacaagc
      421 aaaaataccg ccaaacattg cccggctttt ttcgcccttg cagacttatt taccgcggac
      481 tgttaataat cagcctctgc tggtttctcg gcgataccct ccgcgcacag gccgacacgc
      541 tcaacgggga ttctttgaag gcgggtcctc ccggaaaaaa ggaaagcaca tcgcaacttc
      601 tcggccaggt tacctaccac gcgaaagatt ccatgctctt cgacctgccc gcccagaaag
      661 tatatctctg ggacagcgcg acagtggatt accaggacac gcatctttcg gcgtatttcg
      721 taacgatcga tttcaaaaaa aacatcgtca tcgcggaagg gaaaaaagat tccaccggca
      781 attacatcac gaaagcgaat ttttcggata acggcgaagc gttcgaggcg aacaagcttt
      841 cctacaatta cgtaaccgga aaaggaaaga tcatcgatgt catcaccgaa cagggcgacg
      901 ggttcctgca tgcgcaggtg gcgaaaaagg atacttccgg aacgatctat gtgtataaag
      961 gctattacac gacgtgcgat ctcgagcatc cgcattttgc aattcgtgcg cggaaactca
     1021 aggtcatcca ggacgataag atcattaccg ggcctgctta cctcgaggtc agcgacgttc
     1081 ccacacccat cgctgttccg ttcggcttct tccccaacaa aaaaggaagg gcgtccggga
     1141 tccttattcc cacctacggt gaatcgccta cgctcggttt tttcctgaaa gacggcggtt
     1201 attactgggg aaaaagcgac ctgttcgatc ttgcgctccg cggcgatatt tactcgaaag
     1261 gcagctgggg cgcgaagctg tacacgaatt acaaagtgcg ctacaaatac agcgggaatt
     1321 tttcactgaa atattcgcgc attctccagg gcgaccgcga gcttccgaac cgccgtatcg
     1381 ccaatgactt tttcataacg tggtcgcata cgcaggatcc gaaatcgaat ccgtccatcc
     1441 gtttttccgc gaacgtccgg ggcggcacgt cgacgtacaa ctcgtacaac tccaccattc
     1501 ccacggatta tctttcgaac accttcaact cgaatatttc ctggtcgaag tcgtggaaat
     1561 tcggcgcgct ttccgccaac ctgcgccacg accagaattc gctcacgcat aatgtgtcca
     1621 tgaccctgcc gcagatcgcg tttaccgtga accgttttta tcctttccgc aacccgaaac
     1681 gcgtcaaaca gaaatggtac gacaaggtcg gcatgagcta caccgccgat ttccagaacc
     1741 tgctcagcat cggcgacagc tcgctcagcc tgcggaacgc cgattacatc acgcagcatt
     1801 tccgcaatgg catccaccag tcgctgccca tttccacgtc ctttaacgtg ctacggcatt
     1861 tcacgctcac ctactcgctt acgctgaatt ctatcacgca gttcaattac gtgaacaagc
     1921 gctgggacgg cgagcaggtg gtgacagata cgatcaacgg cgcgaaagtc agtcttgacg
     1981 gaagcacgag cctcacgctg agcacacgcc ttttcggcat gtacaagctg aagcagggcc
     2041 gttacacggt catccggcac acgcttacac cgaacctttc gttcagctac atgcccgact
     2101 tcaccgatcc gcgcttcggc ttttacaaaa ccgtgcagac gaattcggtt ggcgcgtcgt
     2161 cgaaatattc cattttcgag aacggcattt acggcgcaac gccggccggc cgcatcggct
     2221 cgatcgggct gaacctcatc aattcgctgg aagcgaagct tcgtcccgac gaaagcgaca
     2281 gcagcaaaac ggaaaaacgc acgacgctca tcgatgcctt caacgtggcg ggatcgtaca
     2341 atctgatcgc ggatcatttc aactggtcga tgctttctgc cggtttccgc acgaagctgt
     2401 tccacaaggt tgatgtgaac acttctttcc tcgccgatcc ttaccgcgtg agcagcgaag
     2461 gcgtgcgcat cgaacgtttc gaatggcggg aaggaaaacg tctcgtgcgt ctcaccggcg
     2521 caaccgtttc tctgaatacg agcctgcgca aaggcggttt cacttcttcg accacgcaca
     2581 catccaatcg cggaacacag caggagttgg acatgatcaa cgccaacccg aacgcgtatg
     2641 tcgatttcaa catcccgtgg tcgctcaatt tcggttacag cctcaactgg tcgaaaccgc
     2701 tgctgacaca aacggtgacg cagacactgc acgtttccgg cgacgtgaac gtaacaccta
     2761 aatggaaggt cggcttcgat tcgaactacg acatcaaaaa gcaggaattc ggcttcacct
     2821 cgctcaatat ttaccgtgat ctccattgct gggaaatgca gttcaactgg atcccgttcg
     2881 gtccgcggca gagctacaac attaccatca acgtgaaatc atcggtgctg caggacctgc
     2941 gcctgacgcg gaaaagagac tggtacgact tcacgcagca gtaattttca tccatccttt
     3001 ttttacctcg ccggtaatcg ccggcggtca ttcataccgg taaatgcgca tgttaaccgc
     3061 aatccggtat ttttgttcac accaactgct cacaccatgc gtaaattttt cttttccgct
     3121 gcggccgtca cctgtgtcgc tttcattttt tcattcacgg ataatgcgcc tgtcaaagta
     3181 aatcccggcg acaacgattc cactgtcgtg gccaagatct ataacgaagc gctggcgaac
     3241 ggccaggctt acgacgtgct gtccggtctt tgccagaagg cgccgaaacg gctcagcggt
     3301 tcgcccggcg cggcggtcgc tgtgaattac atgaagggcg tcatggatag ttacggtttt
     3361 gataaagtgt ggctgcagcc ctgcaccgtg ccgcactggg tgcgcggaaa cccggaatcg
     3421 ggaaagctca ttttcatgac cggcacgaaa aaaacgcagc acaccgtgaa cgtgtgcgcg
     3481 ctcggcggaa gccagcccac gccggacaaa ggattgcagg cggaagtggt ggaagtaact
     3541 acgttcgcgc agctcgattc gctcggggaa gcaggcgtga aagggaaatt cgttttttac
     3601 aatcacaagt tcaatccgac gacgatttcc actttcagcg catacgggga agatgtgcaa
     3661 taccgctggc agggtgcaac ctccgctgaa aaatacggag ctgtcggtgt gatcgtgcga
     3721 tcggtcactg cgtccatcga cacgtgcccg catacaggcg cagaacgcag cgattcgctc
     3781 gccggtaaaa ttccggcatg cgccatttcc accgttgatg cggattacct cgacgaccag
     3841 atccgccacg gcgtccggcc cgaatttttc ctgaagatga actgccagtg gcttccggat
     3901 gaacattcgt tcaacgtcat cggcgagctg ctcggaagcg agcacccgga acaggtgatc
     3961 gtcgtgggcg gccatctcga cgcgtgggac ctcggcacag gcgcacagga cgacggggcc
     4021 ggctgcgtgc agtcgatcga agcggtgcgc attttcaaaa cgctcggcat tcgcccgaaa
     4081 catacgatcc gctgcgtgtg cttcatgaat gaagagaacg ggggaaaagg cggaaaagcg
     4141 tatgcggatt cggctgcaat caaaaaagaa aagcacatcg ccgccatcga atcggatgcg
     4201 ggcggattca gtccgcgcgg tttcgggctc agcatgtcgc aggaccagcg acgcgagatc
     4261 gaaggatgga agcctttgct gcttccttac ggcatttatg atttttcagg cgaaggcggc
     4321 ggatccgaca ttgacccgct gcagaaacag ggcgtgccga tgatcggcct gtggcccgat
     4381 aacgcccgct attttgatta tcatcacacg gagatcgaca tcatgaagaa cgtgaacaaa
     4441 cgcgagctgc tcatgggcgc ggcagccatg tcctcgctca tctatctcat cgacaaatac
     4501 gggctgtgac ggcgctttat agcattatcg aaaaaagcga tagcgctttc gcgcagttgt
     4561 gactaaattt gtgctgcgct aattcattca ctactcacat aaaaaacaag aacatggcac
     4621 ttcagatcac agatgcaaac ttcgaggaac tcgtcctcaa atccgataag cccgttatcg
     4681 tcgacttctg ggcagaatgg tgcggcccct gccgcatggt aggtccgatc gtggaagaga
     4741 tcggcaacga ctaccaggga cgtgcggttg tcggcaagat ggacgtagac gcgaacgcga
     4801 atattccgat gcagttcggc atccgcaaca ttcccacgat ccttttcttc aaaggaggaa
     4861 agctcgtgga caagcaggtg ggcgcagttc cgaaagcagc gctcgtgcag aagctcgaag
     4921 cacagctgta aacaaagctt atccaacagc aaagccctgg tggtcgcacg actgccgggg
     4981 tttcttttta tttctctttt cgttattcgt caatggcgtc attgaaaaaa acgatgaaag
     5041 cggaacaatg catccttcta ccttgcggac gtatccacat tttagcaacg caaaaaaatg
     5101 acagcagaaa tcacagacca gaattttgat gagctcgtga tccgtccggg aaaaaccgtt
     5161 gtgctcgatc tttgggcgga atggtgtcca ccctgcctga agctgggacc gatcatggaa
     5221 gagatcgcga aagacttcga aggaaaggcc ctcatcggca aggtgaacgt cgatcgcaac
     5281 ccggagattt ccgttcagtt cggcatcagg aacatcccgg ccatccttta tttcaggaac
     5341 gggaaactgg tggacaaaca gatcggtttt gtaccgaaag aaacgctcgt gcagaagctc
     5401 gcagcgcagc tttgaaatac ggccggggaa gacaagacat tttgcagagc gctgtttttt
     5461 atttcatacg gttgaatgta cctttacaaa aatggaactc aaccaccagg aactgcagca
     5521 attctcgcac ctcgagcttc tcgcacgcca ggtggtggaa ggtttcatca ccggcctcca
     5581 caaaagtcct ttccatggat tctcggtgga attcgccgag cacaggctgt acaacagcgg
     5641 ggaacccacc aagcacatcg actggaagct ctacgggcgt acggacaaaa tgttcgtgaa
     5701 gcggtatgag gaagagacga acctgcgctg ccagctcatc atcgacaatt cctcttccat
     5761 gcatttcccg ctgccggaaa aagggaaaga gacggacaac aagatcacgt tctctgtcaa
     5821 atgcgcggcg gcgctcatcg agctgctgcg catgcagcgt gacgcagtag gcctgaccgt
     5881 gttcgacgaa aaagtttccg tgcacacgcc ggcgcggagc agcacgatgc accacaagat
     5941 gctgttcagc gagctcgaaa aacttctcac gcccgaaaaa ccggaagagc accggcgcag
     6001 gacattcgcg atcgaagcgc tgcacgaagt ggcggaaagc atccaccgcc gttcgctggt
     6061 catcatcttc agcgacatgt tcgattcacg ggaaagctac gacgaccttt tttccgcgct
     6121 gcagcacctg aagcacaacc ggcacgaagt gatccttttt catgtcacgg ataaaaagca
     6181 cgagctggac ttcgactacg ataaccgccc gcatacgttc gtcgacatgg aaaccggcga
     6241 agaggtgaaa gttcatcctg cggaagtgaa gtccgtttac ctgaaatcca tcaacgattt
     6301 ccgccacgag ctgaagctgc gctgcgggca ataccgcatc gatttcgtcg aagccgatgt
     6361 gaacgaaggt ttccgccagg tgcttctccc ctacctgctc aagcggacaa gaatgatgta
     6421 agctgcacgg aacgcccggc gcatttcttt ttcattttcc gcaaaaatta cttttgtctt
     6481 cctaaagaat aacgacgcta agaaacacat acctttgcat cgtttttttg tgcacggaaa
     6541 tgtccagaga cgaccatcag catctgcaca gcaaggttac tttcgcaggt ttacttgtca
     6601 cgttaggaat catattcgga gatatcggaa cgtctccgct gtatgttctt aaatccatta
     6661 ttgaaacccc tgacgttcac ggcagggtcc ttcccatcaa cccggaaatc gttctcggcg
     6721 gattgtcctg catcgtatgg acgctgacgc tgcagacgac gatcaaatat gtcctcatca
     6781 cgctccgcgc ggataacaac ggtgaaggcg ggatcttcgc gctgtacacg ctcgtccgcc
     6841 gcacgaagaa aaaatggctg ctgttcccgg ccatcgtcgg aggcgccatg cttctcgccg
     6901 acggcatcat cacaccgccc atttccgttt catcggcgat agaaggtttg agcgtattca
     6961 accagaacat caacaccgtt cccatcgtca tcggcatcat cgccttcctg ttcgtcatcc
     7021 agcagttcgg aacaaagttc gtgggacgtt ccttcggccc cgtgatgttc gtctggtttt
     7081 ccatgctggc ggtgatcggc acgtggatga tcctgaaatg tccttccgtt ttgtgggcgc
     7141 tgagtccgca ttacgccgta atgctgatcg cgaagcatcc cggatcgctg tcgctgctcg
     7201 gtgcggtttt cctgtgtacc accggtgcgg aagcgctgta ttccgatctc ggtcactgcg
     7261 gaagaagaaa tgtacgcgtg agctggacgt tcgtaaagac cgcgctgctg ctgaattatt
     7321 tcgggcaggg tgcatggctg atcatgaacc agggccacat gctttccgaa ttcaagggag
     7381 acaatccttt ctattccatg atgccgccgt ggttctacgg aacggggatc gtcatcgcca
     7441 cggctgccgc cattatcgcc agccaggccc tcatcagcgg atcatttacg ctgatcaacg
     7501 aagccatccg cctcaatttc tggccgaaag tgcgcatcaa gtacccgacg gaactgaaag
     7561 gacagctcta tattccttct gtgaactggc tgctgatgat cggctgcatc gctgtcgttc
     7621 ttcatttcaa aagagccggg gaaatggaag ccgcttacgg tcttgccatc atcatcacca
     7681 tgctgatgac gacgctgctg ctgaactact atcttgtcct gcgccgcacg ccgcgaatac
     7741 tcatcttcct gctgcttttc gtttacctct ccattgaaag cctgttcctc gttgccaatt
     7801 ccgtgaagtt catccacggc ggctacataa cgctcatgat cgccagcctg cttattctcg
     7861 tgatgtggat ctggtacgac gcgcgaaaga tccgcaaccg ttacgtggaa ttcgttcatc
     7921 tcgaccagta cctcccgctt atttcagagc tcgggcacga ccagtccatt ccgaaatacg
     7981 ccacgcacct cgtttacctc acgagcgccg acaacaagga cgagatcgaa tcgaagatca
     8041 tctattccat cctgcagaaa aggccgaaac gggcggacgt gtactggttc gtgcatgtgc
     8101 atgtcaccga ccagccgtac accacggaat acaaagtgac ggcgatgcac aagaaggata
     8161 ttttccggat cgatttctac ctcggcttcc gcgttcccac ccggatcaac ctgatgttcc
     8221 gcaacgtcgt ccaggaaatg gtgcacaacc acgaagtgga catcaccagc cgttacgaat
     8281 cactgaaccg gaaaaatgtc atcggcgatt tccgtttcgt cgtgctcgaa aaatacctgt
     8341 cgaacgagaa tgacctcccc gcttatgaaa aggtcatcct cgatatttat tacctgatga
     8401 agcccatcac gctgtcggag gaaagcgcgt tcgggctcga caccagctct gtttcggtgg
     8461 aaaaagtccc catcgtcatc gccccgcaga aagagctgca ccttaagcgc ctcgactgat
     8521 cattcggttt atcttacctt cgcagggcat tgaccaggaa aaccatactt ccgccggaaa
     8581 acgagctcgt ggccatgctc aggtcgaaga acgagcaggc gatccgcgtg ctgtacgatc
     8641 attactccgc agcgcttttc ggtgttgttc tccggatcgt gaatgaccgc gaaacggcgg
     8701 aggatcttct ccaggaagtt tttgtccgca tctggaacaa tgccgcatct tacgacgttt
     8761 cgagagggcg attgtatacc tggatgctga acattgcacg caacgtaagc atcgacaagc
     8821 tgcgatcgag ggattttcgc aaccacgggc aagtccgcag cctggaagat tacgtaaata
     8881 caatggaaaa ccatcagaag gaaatgaacg tggacgtaat cgggctgaaa aagcttgtcg
     8941 ggaagctcaa acccgagcag cagcagctgg tggacctgct ttatttcaac gggtatacgc
     9001 aaaccgaagc cgccgaaaaa ctcggcattc cattgggaac agttaaaaca aggatcaaat
     9061 ccgccctcgc cgcgctgagg gaaataatgc aatgacgccg caggaactca tcggatcagg
     9121 aatcctggaa gcttacgcaa caggatgtgc cacccgcgaa gaaataacgc tcgcggagga
     9181 aatgcttgcg tcttctcccg aagtacgggc ggagctcgca gcaatccgtg agacgctgga
     9241 aaaatatgca tcgctgcatg ccgccgaacc gcctgccggg acagaggaaa agatccttgc
     9301 cgcggttggc gtttccaggc cggccccgaa gggaaaagtc attacgctcg aagcgcggct
     9361 gttccggcgc ctgcgcaatt tcgctgcggc cgcttcgctg ttcgtcattt tcagcgctgt
     9421 cttcagcctg ctcatgtatt tcgaaaaaag caacaaggca gaagagaatg acgcacttgt
     9481 cgcacagctg aacaggctgc agtcggatta tgattccact tcgaacatgc tgaacaccgc
     9541 cattgccgac cgcgaccagg tgaaacatga gatgtccatg ctgcgcgacc cgatgacgga
     9601 aaccatcacg ttaaaaagca tgatgaaaga aaaaccttcc ataaaagcga tggtgcactg
     9661 gaacaagaac gacatgatgg tggccgtcga cccgatgacg ctgccgccga cgccttccgg
     9721 catgcagtac gagctctggg cgatcgtgga caacaagccg cagagcgtgg atgttttctc
     9781 tgtaaaggaa aacccgggca tctccatgct gatccccgtc cagaaagccg atgcgttcgc
     9841 cattacgctt gaaaaggagg gcggaacgac tgttcctacg ggccagatgt atgttatggg
     9901 ccatgccggc agcaccaatc cctgaatttt cgatatttgt gcaactcctt ccctgacgtg
     9961 aaaagaacaa ttttcattct tctgctgttc ccgctgctct tcgggctggg ccggtgcaaa
    10021 aacgatgtca acctgctcac cgactacaag aattacctgg tgtgctacgg tcttcttgac
    10081 ccgaatgaca cggttcacta catccgtctt tccaaagttt ttctcggaga aggaagcgcg
    10141 tacgacatgg cgtcgaaccc ggatacggcc aatttccgcc ccggcactgc tgacgttttt
    10201 gtggaacgct ggctgaacgg tgtgatgatc acctcgtgga gactcacgcc cgacacaacg
    10261 atcgcacggg atacgggaat tttttccaat ccttaccagg tgctttaccg cggaaatttt
    10321 cccgtgctga aggacggaag catttacaaa ctgtatgcga aggatgttgc gcgcggggac
    10381 attgcgcaat cggaaacgaa gatcgtgcag gatcctgtga tcacttatcc cgccggaaat
    10441 ttcacaccgc tcaatcttta cgatacgacc acgatccgtt ttggaataaa aacgggaacg
    10501 aacggtgtgc gttataatct tttcgtccgc ttccattaca aagaacaatt cgtgttcgac
    10561 acaacggaag tgtccgacaa atacatcgac tggcagatcg ggacgaaaga cgcttcggat
    10621 gaccttggcg gagaggcgat cgattttacg atccgcagga ataatttcct ttcggccgta
    10681 gctgcgctga tcccggtgaa ccccgatgtg cggcgcattg ccggaacgct cgatttttat
    10741 tacacgggcg catcgtccga cctgaaaaca tacatggatg tcggcttcgc caacagcaac
    10801 tccacttcac cgatcgcgac cttcaccaat attaccgggg gacttggact tttcgcttca
    10861 cgcaccacga gcatcttccc caactattcc atcgacgcca atacaaaatc cgcgttgcgc
    10921 aacaacacgg aaacagtgca gctcggtttt atccggtaaa attttattcc cggatgaatt
    10981 tcgtccggct gacttccccg ttttcacctt cgatcaccac cgaataaaat cccggtgcga
    11041 aaccggagac agggatcgtc gccattttcc cgctttgctt ttcacggtaa acaagctggc
    11101 cggtggcgtt gaagatgctc accgtgctgg tcccgttcgg ggaaggaaga tcgaagttga
    11161 caatgtcatc cgaaggatcg ggccacaggc gaatgctgct gttctcatcc cggaaatacg
    11221 gaacacccgt gacgtacgtg atggcgatca tcgaaagatt cggatgataa atgccggaaa
    11281 ccgtttcaaa tcctccgccg gtgaagagcc agtcctggtt cgctgccatt gtcacgggca
    11341 tcacatcggg cgcggggttc catgtatcga actgctggtt gttgtaatcg taatcacaga
    11401 gatgcgggaa agtggaatta ttcacgacgg tgaacatgcc gctgaagaaa atgtcactgc
    11461 cgtaatgcgt catggtgtga acgtacgcat ccgatccggg gttgaacgcc tgcactgttc
    11521 cgtcgagggt gtccaccgcc gccacgtgat tcctcggctg gcctgccaca tcggaaaacc
    11581 atccgccgaa gtagacgttc ccgcccgcga tctccatatc gtaaatacca tcatccgtat
    11641 ctgcgctcca gctgccgtcg gccacgcccg ttgatttgct gaggcgcgaa agattcttcc
    11701 gcgggtaagc gccgatggaa gaatagaacc cggcgacata aacataattg ccattagcga
    11761 tgagcctgtt cactgttccc tggcaattgg gattccaggg cgtcgcggac tggctcgcga
    11821 tctttatcgc tgcaatgttg ttcctctgct gtccgccggc ctgcgtaaaa ttcccgccga
    11881 tgaataacgt cgtgtcgtcg ctgtccatcg tgcgcacatt cccgcttacg ccgggattga
    11941 atgtcgtgac cgtccgcgtc gcgagatcgt acgaagcgat cccgttcctc gcctgctggt
    12001 cgacgaccgc aaaatttccg gcaatgtaga gatccgaatg atcaacggtc atcgcagaaa
    12061 ttgccgaagc caccagcgga tgccagctgt ccggacgaag tgtgctcagc gggatggcgc
    12121 acaggttttc ccggtcgacg ccgcctgctc ctgaatattc tccacccaca agaacattcc
    12181 cggaaaagcc gggaagcatg gcattcaccg ggccgtcgat cttcgcgttc cattgtttga
    12241 tcgttcccgt tccggtggaa tcaatgaacc cggcatggta aacagaatcg gtgccgagca
    12301 tcgagaaatt tccgccgatg cagaaattaa cgccatcggc cgtgcgcacg cagttcacag
    12361 tgccattcgc agaaggattc catgcgtaaa ggctcatcga cgtatcaatg cacgcgaggt
    12421 tctggcgcgg gtttccccga acggatgtaa atgtcccgcc gataaaaatt tttccgttga
    12481 cgaattccat cgtacgcacc ttgccggaaa caagcgggat cagggaagtg ttcaccaccc
    12541 cgttgctttt gacgatcgcg agtcctgaaa ggttgaggct gccgagcgca gtgaaatcgc
    12601 cgccgatgat gatgttggag gcaaaggtgc ggatggtgcg gacaggtccg ttcgggagct
    12661 gggaacattg ccagggaaga ttattggcga aagcggaaga accggttgcc gtatccagca
    12721 ttccgaggaa aggaatgttg tacccgccga tcgcggtgaa ctggccaccg gcaatgacgt
    12781 tcgagttttc cggccagaaa gcatagatcg tattatcggc gcgcggatcg aaatcggtca
    12841 actcgccgag cgaatcgatg cgtgcaccgc gtccgcgcgt ctgcccgaaa atattggtga
    12901 aatcaccgcc gataaaaatg catttcagga aattttcaat gcagaacact tcaccgttcg
    12961 ggttcggcat gaacgacagg tcgacctggc ccgaaggagt gaaacgaaaa agattgctgg
    13021 cgcttacatt tccgaccctg ctgaattttc cgcccacgta aacgtagcct gcgctgtcgc
    13081 ggcagatgca attcacacgt ccgtatacga gcgggtacgg ctgcacagcg gaagcattgg
    13141 ccgtgtcgaa cggaacgaga cacccggtca ccgggctcac catggaaaaa tctccgccga
    13201 cgaacagcgt ggaatctttc agcgcgaccg cactcacggg accgttcggg atccagagcg
    13261 acggatttgc attctgtgaa aagagctgcg tacatcccgc gcagaccgcg accgcaacca
    13321 gtaatctttt gaaaatgacc attgatacca ccgggttaac cgcccaaaaa taagcaatta
    13381 gtgaacggct ttatatgtta cttttgtggc ttaccggaat gttcatcacc caatgaaaaa
    13441 gtttttttta ttcgcttcgc ttgccctttt cctgtcgacc tgcaccactg atgtcgacct
    13501 gctggacaac tggaaggaaa ccaccgtgat ttacggattg ctcaaccagg ccgatacgat
    13561 gcagtacatc cgtatccaga aagcttttct tggtcctgac aatgcctaca ccatggcgca
    13621 ggaattcgat tcaatcaatt acatcaacca gctcgacgtt cgtctcgaag aactttacaa
    13681 cggcaacgtg atcaattcct ggccgcttta tcccgatacg atcagtaaca aggctcccgg
    13741 tatcttcagc tatcccatgc aggtggtgta tgcgcttcat acaggcgcgc cttcattctt
    13801 caataccgct caccagtatc atctcgttgt gaagaacaac cagaccggca atacggcgga
    13861 tgcgacaacg agcctcatga gcgacttcaa catgatcgcg ccgcagggcc cttccatcag
    13921 catcgtgaag atcaattcca acaccatgaa ttccatttca tggggctcct gcccgaaagc
    13981 gcgcattttc caggtcggcc ttacgttcca ttattccgaa acagacgtga acaacaacac
    14041 tgtatcgaaa gcaacgcctg aatggatgct cggaacagtt tacaccgatt cgctttctgc
    14101 gctcgatccg caatcgacca ccttcgaccc gaacgacttt taccgtttca tcgcgaacaa
    14161 catcccgcag gacccgaacg tcgtttcccg caaggacgaa ttcatcacga tcaccgtata
    14221 tgcaggtggc gaagcacttt acacctatat ggaaaccaac ggagcgtcca actctgtggt
    14281 acaggaccgg cctttttaca ccaacatcaa taacggtctc ggcatcttct ccgcacgtta
    14341 cggaaaatcc aaaacgaacc ttacgctgac gccgctcacc atcgatacac ttgccatggg
    14401 ccagttcacc tgtggtctcc ggttcaagga ccggaacggc gtgatctctg ccggctgcca
    14461 gtaatcctta ccgcttcgca ttacacaaaa ggcgctcccc ggagtgcctt tttgtcatat
    14521 aatatggcag aaaaatgctg ttgtgtcccc gattcgcgca gaaaactgcc aaagaaccgg
    14581 gtggcatagt gattgactat caggagcaga ctaaaaaacc aacgaaactt taatacttat
    14641 cagttatggg aaaaatcatt ggaatcgacc tgggaactac gaactcctgc gtagctgtga
    14701 tggaagggaa cgaacctgtt gttattccga acagcgaggg acgccgtacc accccgtcga
    14761 tcgtcgcctt tgtagaaaac ggcgagcgca aagtgggcga cccggccaag cggcaggcca
    14821 ttaccaaccc acgcaaaact atttattcga tcaaacgttt catgggtcat ctctatgacg
    14881 aggttaccga agaaataaaa cgcgtgccgt attctgtcgt gaaaggcgat aacaacacgc
    14941 cccgcgtgca gatcgacgac cgcaaataca caccgcagga aatttccgcc atgatccttc
    15001 agaaaatgaa gaagactgcg gaagattttc tcggaacgga agtgaccgaa gccgttatca
    15061 cggtcccggc gtacttcaac gatgcgcagc gccaggctac gaaagaagcc ggcgaaattg
    15121 ccgggctgaa agtccgccgc atcatcaacg agccgacagc agccgcgctg gcttacggcc
    15181 tcgacaaaaa acacagcgac atgaagatcg tcgtgttcga ctgcggcggc ggaacgcatg
    15241 acgtttccgt cctcgagctc ggagacggcg tgttcgaagt gaaatccacc gacggtgata
    15301 cgcacctcgg cggcgacgac ttcgaccaga agatcatcga atggctcgta gccgaattca
    15361 aagcggaaaa cggcaacctc gatcttacga aagacccgat ggcgctccag cgcctgaagg
    15421 aagctgctga aaaagcgaag atcgaactgt cgagctccac atccaccgag atcaaccttc
    15481 cgtacatcat gccggtggac ggcatcccga aacacctcgt acgcacgctc agccgcgcga
    15541 aattcgagca gctcgtggac gaccttatcc gccgcaccat cgctccgtgc aaatctgcgc
    15601 tgcagaatgc aggactgacg gtgaacgata tcaacgagat cattctcgtc ggcggatcga
    15661 cacgtatccc tgccattgtt gacgccgtgc agaaattctt cggcaaagcg ccttcgaaag
    15721 gcgtgaaccc ggatgaagtt gttgccgtag gcgcagctat ccagggcggc gtgctcaccg
    15781 gcgaagtgaa agacgttctc ctgctcgacg taacaccgct ttctctcggt atcgaaacgt
    15841 atggcggcgt ctgcacgaaa ctgatcgaat ccaacaccac gatcccgacg aaaaaatcgg
    15901 aaacgttctc gacggcgtcc gacaaccagc cttcggttga aattcatatc ctccagggcg
    15961 aacgcccgat ggcgaaagac aaccgcacgc tcggacgttt catcctcgac ggcattcctt
    16021 ccgcaccgcg cggcgttccg cagatcgaag tcacattcga catcgacgcg aacgggatcc
    16081 t
//
LOCUS       DASTSF010000366        12476 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_480999,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000366 DASTSF010000000
VERSION     DASTSF010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 12476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12476
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_480999"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..317)
                     /locus_tag="VFU15_10445"
     CDS             complement(<1..317)
                     /locus_tag="VFU15_10445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718246.1"
                     /translation="MKLPEYGIIAFASVMLSGLTVLAVKIPKNPMRLLLAFSGAFLFA
                     LSLLHLIPELYRTEHFNAGIWILAGFLVQLLLEYVSQGIEHGHVHLHEHAHGHGHGKT
                     KIPL"
     gene            complement(319..1074)
                     /locus_tag="VFU15_10450"
     CDS             complement(319..1074)
                     /locus_tag="VFU15_10450"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU4718247.1"
                     /translation="MQAETAMNDESWYLEWFDSPYYHLLYDNRDETEARDFVHRLFSF
                     LRPSLPARVLDAGCGSGRHARVMNELGFEVTGIDLSPANITRARRSENDTLSFFVHDM
                     RRTFRVNYFDYAFNLFTSFGYFRSNRDNVRVIDSLHSALKKDGILVLDFFNSSCVEKM
                     IASCNSGSKISGGIEFTWKKKIVSGFIEKEIRFSDKGKDFHFTEHVRLLKPDDFRALF
                     ENKFSVLHTFGDYRLAEFNEDESPRLIFIAKKN"
     gene            1194..4529
                     /locus_tag="VFU15_10455"
     CDS             1194..4529
                     /locus_tag="VFU15_10455"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HEU4718248.1"
                     /translation="MIFFSRPKHFTAILLFLPLLVFSQDYKFSHIGVENGLSQSVVNC
                     TLQDRLGFMWFGTQEGLNRYDGNNFTIYKRNPEDSNSLSNNFIYSMCEDRNGILWIGT
                     NGGGLNSFNPATGKFIHYMNDPKDNSSISNDIVRAVLADDDGTIWLGTDGGLNRLDPA
                     TGKCTRFVNNPNDLYSLSSDNITAMTKDASGNLWIGTYGKGICMFDKASRKFFNYQIT
                     DKELNDLYSAAVVTDEKRKSQCSIVRCLLFGDATHLWIGTNGGGVEIFNTEKHIFESI
                     LFPGVGKESISNTLVLSLCDDGENNIWIASFESRIDIYNRATGHIRHYVPVEHDFYAL
                     NSSGIRTIFRDANGNMWIGTNGDGINVYFTSTSTIEHIRKSEDPSAGNNTLLSNKIMC
                     VMEDKDDVIWMGTSGGGLTTYDRKTNKYTQHPELSTSINNSVISLLQSSDGKIWVGTY
                     GEGLNCYDPVTKKITSYSPDNRLDDGNILCIAEEPSTHYIWFGTFGAGMYRLDPKTDS
                     LKQFTKDSSGLSSDYIYTIFFDKSGTLWIGTRTGGVMMRDPLTGKFISYVHDDQDKNS
                     ISNNIVYCVAEDNAGDLWMSTANGLNRFDRKTKKFTTWYERDGLPSDNIYSVVPDEKG
                     NLWLSHNKGLTRFSPSAAEGFRFKNFGPGAGVQSSEFNQGAFFSRNGELFFGGQKGLN
                     IIDSRNVEPKQPPPPVYIISYRRFGKVIVKDTNIIYSHDLTVSWRDNNFLFELAALDY
                     VDPSKNLFQYKLDGLDDDWSAPTTNRFVSYTNLPGGNYTLWVKAANCNGNWSEPKMLL
                     HITVNPPFWKTNWFYSVCVIVLIGGVFGFIRYRTSAIKKENRILEAKVAERTHELAQK
                     NADITASIEYARRIQSAILPDLSYIYRHLPESFVLYKPKDIVSGDFYWFNYNEKTGRI
                     MLAVADCTGHGVPGALMSIIGHNLLNEIVAEKGIDEPGQVLEKLNEGVRAALKQNLHE
                     KDTTDGMDIALLSIDRAKNEIAFAGALRPLIFIRKGLLSKIDSDRFPIGGSQDTRERK
                     FNVHRLKVEKGDTLYMFSDGIADQFGGEKGKKFMMKRLLETLTSLQEHPVKEQAQVLD
                     NVFETWKEGFEQVDDVLIVGIRI"
     gene            4684..5490
                     /locus_tag="VFU15_10460"
     CDS             4684..5490
                     /locus_tag="VFU15_10460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718249.1"
                     /translation="MKRIFLPVAVIVIAALAINSCKKDTTDTDTTAETDNSICENEFM
                     RTLPTVNSIAIDDAGVHRTGPGNTMSSSCPSHYIIGTQWPKTLVIDYGTGCTDPVDGK
                     VRKGRIRCVLSNSWDSIGTTCTMSLDTFFVGAIQFEGTCTITRTGTNTFHKVVTNGKC
                     TKSGTSPWTVLWDCDRTIEYQSGLTDSTQVQVIKITGTNHGTDRNGKTWSSDITTPII
                     RDMDCSWLTSGVVDITPEGKPTRTVNFGDGTCDNKGTITVDGSTFEFTMQ"
     gene            5617..6186
                     /locus_tag="VFU15_10465"
     CDS             5617..6186
                     /locus_tag="VFU15_10465"
                     /inference="COORDINATES: protein motif:HMM:NF038262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SiaB family protein kinase"
                     /protein_id="HEU4718250.1"
                     /translation="MMVDIYDFYDKMERNNIMLSFKGDITSELLTSILQIMESKLDTM
                     QEEPKIKKKVYNVLVECLQNLYHHMDEIPRSLNGNSVAGDGESLRSAIFMIGKIDNQY
                     NIITGNYILTGNVDKLRTRLDEINKLSKEELKDYYKAILNNGEMSEKGGGGLGMIDIA
                     RKTGQKLNYHFMPVDDIYSFFSLNIKIAQ"
     gene            6210..6590
                     /locus_tag="VFU15_10470"
     CDS             6210..6590
                     /locus_tag="VFU15_10470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010420925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1987 domain-containing protein"
                     /protein_id="HEU4718251.1"
                     /translation="MERIVIEGTPKTPTIHFDMSTGQLEIRGRSIPENSIEFYKPLVE
                     QLERYAQKPQANTNVNIQLEYFNTSSSKCILDVFKKLEAIHKGGSAIIINWYYEQDDE
                     DMLEAGEDYQAIISVPFKMIEVAE"
     gene            complement(6657..8042)
                     /locus_tag="VFU15_10475"
     CDS             complement(6657..8042)
                     /locus_tag="VFU15_10475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HEU4718252.1"
                     /translation="MFAIVDIETTGGSPERDRITEICILIHDGLTVVDKFSTLINPER
                     RIPEYITRMTRISDEMVRDAPKFWEVARRIIEMTEGKVFVAHNVNFDYGFIQAEFRSL
                     GYQWKREKLCTVKMSRKLLPRRISYSLGRLCESLGIYIEDRHRAAGDAEATAKLFDIL
                     LAKKSEHPTYKRHNLEDLNTTKVDKIKLYVLRKLPEECGVYYFLNREKEIIYIGKSRN
                     MRTRAIAHFANKKTHYKMLDELMDVDFVKTGSELVALLHESSEIKKHKPKFNKARRRD
                     VFTHSIEWKKEKNEALALEIVPYDEAEHPLLSYTNYTTAREKLNEWIDEQSLCLNYCG
                     LNPEGGECFQRHVKKCFGLCTGDESVEDYNLRVKKIIAEFETEASVYFIVDKGRHDEE
                     RSLILVENNRYAGYGYFDSSDSFSSPEEMKSVIRRTEYFPDADELLRGWMKQNPRVKK
                     VLVKEGNRIVD"
     gene            8222..9235
                     /locus_tag="VFU15_10480"
     CDS             8222..9235
                     /locus_tag="VFU15_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020530536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-heptose synthase"
                     /protein_id="HEU4718253.1"
                     /translation="MAKHSRKELKRIFDSFEKLRVLIIGDVMIDNYVWGKVSRISPEA
                     PVPVVSVARKEQRLGGAANVALNVQSLGATPILCSVIGVDRNGQLFLELMQQEKLAPK
                     GILKSRDRITTEKTRIIGNNHHLLRVDEEIDHDITPHETGLFLERIAYLLDTQKIDVI
                     IFEDYDKGLITEKLIREVVSRARKLKVPVVVDPKKKHFMNYQGATLFKPNLKELKEGL
                     KVDFDQSSAKELSKAVDLLKTKLKIDMALITLSEHGIYVHGRHVKKLLPAHVRNIADV
                     SGAGDTVVSVAALCLAAGLKPELLAEISNIAGGLVCESVGVSPIDKEQLLEESVQLIA
                     GKN"
     gene            9263..9817
                     /locus_tag="VFU15_10485"
     CDS             9263..9817
                     /locus_tag="VFU15_10485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718254.1"
                     /translation="MRILPFFVPVFAIALLASSFCGKKKKKKQEFTVNGTVLLTHSYC
                     GGIVPPAEFLEAMKTPQPAPGFHLFVKKGNTNVSGSAILDTIVTDAQGHFSVSLPAGT
                     YCIVKDWKAPKYSVPQDNQYAEYDTACYRKEYAQCDYLLNLSANVDSLKLVFHEGCSW
                     EQPCMNYHGPLPPAAHPGGHPHNE"
     gene            complement(9954..10973)
                     /gene="obgE"
                     /locus_tag="VFU15_10490"
     CDS             complement(9954..10973)
                     /gene="obgE"
                     /locus_tag="VFU15_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009196496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEU4718255.1"
                     /translation="MSESNFVDYVKICCRSGKGGGGSVHFLRTAQTAKGGPDGGDGGR
                     GGHVIVRGNKQLWTLLHLKYRKHVIAEDGESGGRANMTGADGKDEVIEVPLGTVAKDA
                     ETGETIFEITEDGEERIIVPGGRGGKGNDFFKTATRQTPRFAQPGEPGREEWKVLELK
                     LLADVGLVGFPNAGKSTLLSVVSAARPEIADYPFTTLVPNLGIVKYRDDRSFIMADIP
                     GIIEGAHEGRGLGLRFLRHIERNSSLLFLVPADSTDIVKDYQVLMHELQEYNPELLHK
                     KRLLGVSKADLLDEELFTATKKDLDKRFLKKFKTPYVIFSSATGKGIQELKDELWKLM
                     NSNEI"
     gene            complement(11024..11599)
                     /locus_tag="VFU15_10495"
     CDS             complement(11024..11599)
                     /locus_tag="VFU15_10495"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007653167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEU4718256.1"
                     /translation="MINIVLSGPPGAGKGTQATKLVDKYGFKQLSTGDIFRAIKKSDS
                     PLAKEVISYMDKGMLVPDELTIRVLEAEVNMYMNQVGFIFDGFPRTIAQAEALDKFLA
                     GKGMIVSAMLALEVNDEELKKRILLRGKDSGRADDQDPQIVEKRITEYNTKTAPLRDY
                     YAAQGKYRSVYGIGDIEEIFAKLCKEVDDLR"
     gene            complement(11620..12159)
                     /gene="hpt"
                     /locus_tag="VFU15_10500"
     CDS             complement(11620..12159)
                     /gene="hpt"
                     /locus_tag="VFU15_10500"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007478471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEU4718257.1"
                     /translation="MDPIQLGDKHFVPYISEEKISAAVKNVANAINMDYDGKHPLFLV
                     VLNGSFLFAADLVRSVKLNCEVSFVKLASYEGTSSSGTVKELIGLNESIAGRDVIIVE
                     DIVDTGNTIAQLVEQLLKREPASLRIASLLLKPEVYKKQIPVDYVGLRIPNDFIVGYG
                     LDYNGLGRNLRDIYVIRNQ"
BASE COUNT         3122 a         3377 c         3141 g         2836 t
ORIGIN      
        1 agcggaattt tcgtttttcc gtgaccgtga ccatgtgcat gctcatgcag gtggacatgc
       61 ccgtgctcga tgccctgcga aacatattcg agaagaagct gaacgaggaa accggccagg
      121 atccagatgc ccgcattgaa atgttccgtg cggtaaagtt ccgggatgag gtgcagaagg
      181 ctgagcgcaa aaagaaatgc tccgctgaaa gccagcaaca gccgcatcgg gttcttcggg
      241 atcttcaccg caagaacggt gagtccgctg agcatgacgg aggcaaatgc gataataccg
      301 tattccggca atttcatatc agttcttttt cgcgataaag atcagccgcg gcgattcgtc
      361 ttcattgaat tccgcaagcc tgtaatcgcc gaatgtgtgg agcacgctga acttattttc
      421 aaacagcgcc cggaaatcgt ccggtttcag caggcggacg tgctcggtaa aatggaagtc
      481 cttcccttta tcggaaaacc ggatttcctt ttcgataaaa ccgctgacga tctttttctt
      541 ccatgtaaac tctatgccgc ccgagatctt gctgccggaa ttgcagctgg cgatcatttt
      601 ttccacgcag gaagagttga aaaaatcaag cacgagtatc ccgtcctttt tcagcgcgct
      661 gtgcagggaa tcgatgacgc gcacgttatc gcggttgctg cggaaatagc cgaaggaagt
      721 gaacaggttg aatgcgtaat cgaaataatt cacgcggaaa gtgcggcgca tgtcatgcac
      781 aaaaaaggaa agcgtatcat tttccgatcg cctggcccgg gtgatattcg ccggggaaag
      841 atcgatgccg gtcacttcaa atccaagctc gttcatcacg cgcgcatgcc gcccgctccc
      901 gcaacctgcg tcgagcaccc gcgccggaag cgaaggccgg aggaaagaaa aaagacggtg
      961 cacgaaatcg cgcgcttctg tttcgtcccg gttgtcgtac aaaaggtgat aatacgggct
     1021 gtcgaaccac tcgaggtacc agctttcatc gttcatggcc gtttccgcct gcatggcgta
     1081 aaattagaac ggagcgcaat acaaattacc cctgtcatca aaatcaccgg ataaaaaagc
     1141 cgtatacgcg aaggtttgct aattttatca ccttcacaac gcagccttcc tgcatgattt
     1201 tcttcagccg accgaaacat tttaccgcga tcctgctttt cctcccgctg cttgtttttt
     1261 cgcaggatta caagttcagc catatcggcg tggagaacgg cctttcccag tcggtggtga
     1321 actgtacgct gcaggacagg ctcggcttca tgtggttcgg cacgcaggaa ggactgaacc
     1381 gttacgacgg gaataatttc acgatctaca agcgcaatcc cgaagattcg aattcgcttt
     1441 cgaacaattt catttattcg atgtgcgagg accgcaacgg cattctctgg atcggcacga
     1501 acgggggcgg actgaattcc ttcaacccgg cgacaggtaa gttcatccat tacatgaacg
     1561 atccgaaaga caattcatcg atcagcaacg acatcgtacg cgctgtgctt gccgacgacg
     1621 acggaacgat ctggctggga accgacggag ggctgaaccg tctcgaccct gccacgggca
     1681 aatgcacacg tttcgtgaac aacccgaacg acctgtacag cctcagctcc gacaacatca
     1741 cggccatgac gaaggacgct tccgggaatc tctggatcgg cacgtacgga aaaggcatct
     1801 gcatgttcga caaagcgtcg cggaaatttt tcaattacca gatcaccgac aaggagctga
     1861 acgaccttta ttccgcggct gttgtcaccg atgaaaaacg gaaaagccag tgctcgatcg
     1921 tacgctgtct cctgttcggc gatgccacgc atctatggat cgggacgaac ggtggcggcg
     1981 tggagatctt caacacggag aagcacattt tcgaaagcat tcttttcccg ggcgtcggga
     2041 aagaatccat ttccaacacg ctcgttcttt ccctctgcga cgacggggag aacaacatct
     2101 ggatcgcctc gttcgagagc cgcatcgaca tctacaaccg cgccacgggc cacatccgcc
     2161 attacgttcc cgtcgagcat gatttttacg cgctgaactc ttccggcata cgcaccatat
     2221 tccgagacgc gaacgggaac atgtggatcg gcacgaacgg cgacggcatc aacgtgtatt
     2281 tcacctccac ctccaccatc gagcacatcc ggaaatccga agatccgtcc gccgggaaca
     2341 acacgctgct cagcaataag atcatgtgcg tgatggaaga taaggacgac gtgatctgga
     2401 tgggaacatc cggcggagga ttgaccacgt acgaccgcaa gacgaacaaa tacacgcagc
     2461 atcccgaact ctccacgtcg atcaacaatt cggtgatcag cctcctgcaa agctccgatg
     2521 ggaaaatatg ggtcggcacg tacggcgaag gactgaactg ctacgacccg gtaacgaaaa
     2581 agatcacgtc gtattcaccg gacaaccgcc tcgacgacgg gaacattctc tgcattgccg
     2641 aagaaccttc cacgcattac atctggttcg ggactttcgg ggcgggaatg taccggctcg
     2701 atccgaaaac agattcgctg aaacagttca cgaaagacag ctccggcctc agcagcgatt
     2761 acatctacac gatattcttc gacaaatccg ggacgctctg gatcggcaca cgtaccggcg
     2821 gcgtgatgat gcgtgacccg ctcacgggga aattcatttc gtacgtgcac gacgaccagg
     2881 acaagaattc catttcgaac aacattgttt actgtgtcgc ggaagacaac gcaggcgacc
     2941 tgtggatgag cacggcgaac gggctgaacc gtttcgacag gaagacgaaa aaattcacga
     3001 cgtggtacga acgcgacggg cttcccagcg acaatattta ttccgtcgtc ccggatgaaa
     3061 aagggaatct ctggctgagc cacaacaaag gtctgacgcg gttctcccct tccgcagccg
     3121 aaggtttccg cttcaagaat ttcggacccg gtgccggtgt gcagtcatcg gaattcaacc
     3181 agggcgcatt cttcagccgc aacggggaat tgttcttcgg cggacagaaa ggactgaaca
     3241 tcatcgattc gcggaatgtg gagcctaagc agccgccccc gcccgtctat attatttctt
     3301 accgccgttt cggaaaagtg atcgtcaagg acacgaacat catttattcg cacgatctta
     3361 ccgtcagctg gcgcgacaac aatttccttt tcgagcttgc tgcgctcgat tacgtcgatc
     3421 cttccaaaaa tcttttccag tacaagctcg acggcctcga cgacgactgg tcggcgccca
     3481 cgacgaaccg cttcgtcagc tacacgaatc ttcccggcgg aaattacacc ttgtgggtaa
     3541 aagcggcgaa ctgcaacggg aactggagcg agccgaaaat gctcctgcac atcaccgtca
     3601 accctccgtt ctggaaaacg aactggttct attccgtatg cgtcatcgtg cttatcgggg
     3661 gcgtgttcgg attcatccgc taccgcacaa gcgcgatcaa aaaggaaaac cgcattctcg
     3721 aagcgaaggt tgccgagcgc acgcacgagc tcgcgcagaa aaacgcagac atcacggcca
     3781 gcatcgaata cgcaagaagg atacagtcgg ccatcctgcc cgacctttcc tacatttacc
     3841 gccaccttcc cgaaagcttc gtgctgtaca agccgaagga tattgtttcc ggcgatttct
     3901 actggttcaa ttacaacgaa aagaccggca ggatcatgct cgctgtcgcc gattgcacgg
     3961 gacacggcgt tcccggcgca ctcatgagca tcatcggcca taacctgctg aacgagatcg
     4021 ttgcggaaaa aggaattgac gagcccgggc aggtgcttga aaagctgaat gaaggcgtaa
     4081 gagctgcgct caaacagaac ctgcacgaaa aagacacaac ggacgggatg gacattgccc
     4141 tgctttccat cgaccgcgcg aaaaacgaga tcgcatttgc aggtgcgctg cgcccgctga
     4201 tctttatccg gaaaggattg ctgagcaaga tcgacagcga ccgcttcccc atcggcggtt
     4261 cgcaggatac acgtgaacgg aaattcaacg tgcaccgtct gaaagtggaa aaaggcgata
     4321 cgctttatat gttctcggac ggaatagccg accagttcgg cggtgaaaaa ggaaaaaagt
     4381 tcatgatgaa gcggcttctc gaaacactca cctcgctgca ggaacatccc gtgaaggagc
     4441 aggcgcaggt gcttgacaat gttttcgaaa catggaaaga aggattcgag caggtggacg
     4501 atgtgctcat cgtcggcata aggatatagg tgttcgtgca aaaaactgcg gtgaatttct
     4561 ctctgcccac gtaagaattg ccaaaaccat aattggcgaa ctcccgtaaa aaggtttagt
     4621 tttgcattaa accattggat gccggcttca tccaatccga ggaatcaacc aaaacctgtc
     4681 tcaatgaaac gcattttcct tcctgtcgcc gttattgtta ttgcggccct cgccatcaac
     4741 agttgcaaga aagacacaac tgacaccgat accacggcag aaaccgataa cagcatctgc
     4801 gagaatgaat tcatgcgcac actgcccacg gtcaacagca ttgccatcga tgatgcaggc
     4861 gttcaccgca cgggtccggg caacaccatg agctccagtt gtccctcgca ttatatcatc
     4921 ggaacgcagt ggccgaaaac gcttgtgatc gattacggta cgggctgcac cgacccggta
     4981 gacggtaaag tgcgtaaagg ccgcatccgc tgcgtgcttt cgaattcctg ggattccatc
     5041 ggaacgacct gcaccatgtc gctcgatacg ttctttgtag gtgctatcca gttcgaagga
     5101 acctgcacga tcacgcgcac cggaaccaac accttccata aagtggtgac gaacgggaaa
     5161 tgcacgaaaa gcggcacatc gccctggacc gttctctggg actgcgaccg tacgatcgaa
     5221 taccagagcg ggctgaccga cagcacgcag gtgcaggtga tcaagatcac gggcacgaac
     5281 catggcaccg atcgcaacgg aaaaacatgg tcgtccgata tcacaacacc cattatccgc
     5341 gatatggatt gctcctggct gacatcgggc gtagtcgaca tcacgccgga aggcaagccc
     5401 acccggacgg tcaacttcgg tgatggtacc tgcgacaaca aaggcacgat caccgtcgac
     5461 ggcagcacat ttgaattcac aatgcaataa gccgtgtgaa cgcgttgaaa agccttcccc
     5521 cgggaaggct ttttgccatg tatgcggtga ttttgtttta tggaaataac atttatattt
     5581 gcttaattgt aatccgaaga aagccctggc cattttatga tggtggacat ctatgatttt
     5641 tacgacaaga tggagcgcaa taatattatg ctctccttca agggtgatat aacttcggag
     5701 ttactcactt ctattctgca gataatggag tcgaaactcg acaccatgca ggaggaaccc
     5761 aagatcaaga agaaagttta caacgttctg gtggaatgtt tacagaacct ttaccatcac
     5821 atggatgaaa ttccccgttc tttgaacgga aattcagtgg ccggtgatgg tgaaagcctt
     5881 cgttccgcga tcttcatgat cggcaagatc gacaaccagt ataacatcat cacaggcaac
     5941 tacatcctca ccggcaacgt ggacaagctc cgcacgcggc tggatgagat caacaagctt
     6001 tcgaaagaag agctgaaaga ttattataag gcaatactga ataacggaga aatgtcggaa
     6061 aaaggcggcg gcggactggg catgatcgat attgcgcgga aaacgggtca gaagctcaac
     6121 taccacttca tgcctgtaga cgacatctat tctttcttca gcctgaacat caaaatagca
     6181 caataacaca caaccatatc ctttcaacta tggaacgaat tgtaatagaa ggcacaccca
     6241 aaaccccgac catccatttc gatatgtcca ccgggcagct cgagatccgc ggacgctcca
     6301 tcccggaaaa ttccattgaa ttctacaagc cgctcgtcga gcagctcgaa cgctatgcgc
     6361 agaagccgca ggcgaatacg aacgtcaaca tccagctcga atatttcaac acgagctcat
     6421 ccaagtgcat cctcgacgtg ttcaagaagc tcgaggctat tcacaaaggc ggatcggcga
     6481 tcatcatcaa ctggtattac gagcaggacg acgaagacat gctcgaagcc ggcgaagatt
     6541 accaggccat catctccgtg ccgttcaaaa tgatcgaagt ggcggaataa cagtttcgct
     6601 tcagcatacg aaaacgcctc tcagcaatga ggggcgtttt ttttgtccgc gatgctttaa
     6661 tcaacaatgc gattgccctc tttcacaagg accttcttca cccgcggatt ctgtttcatc
     6721 catcccctga gcaattcatc cgcatcggga aaatattccg tgcgcctgat caccgatttc
     6781 atttcttccg gcgaagagaa cgaatccgat gagtcgaaat agccgtaacc ggcataacgg
     6841 ttgttctcga cgagaatgag cgagcgctcc tcatcgtggc ggcctttgtc gacgataaaa
     6901 taaacggaag cttccgtttc gaattcagcg atgatctttt tcacccgcag gttgtaatct
     6961 tccacggatt catcgccggt gcaaaggccg aaacattttt tgacatgacg ctggaagcat
     7021 tccccgcctt cgggattgag tccgcagtaa ttcaggcaaa gcgactgctc atcgatccac
     7081 tcgttcagct tttcacgcgc ggtggtgtag ttggtatacg aaagcaaagg atgttccgct
     7141 tcatcgtacg gaacgatctc cagcgcaagc gcctcgtttt tttccttttt ccattcgatg
     7201 gaatgcgtga atacgtcgcg gcggcgggct ttgttgaatt tcggtttgtg cttttttatt
     7261 tcggacgatt cgtgcaggag cgccacgagc tcgcttcctg ttttcacgaa gtcgacatcc
     7321 atcagctcgt cgagcatttt gtaatgcgtc ttcttgttcg cgaaatgtgc gatcgcacgc
     7381 gtgcgcatgt tgcggctttt accgatgtaa atgatctcct tctccctgtt caggaaataa
     7441 tacacgccgc attcctcggg aagcttgcgc agcacataaa gtttgatctt atcgactttc
     7501 gtggtgttga ggtcttcgag gttgtgccgt ttgtacgtgg gatgttcaga cttttttgcg
     7561 agaagaatat cgaacagttt cgccgtggct tcggcatcgc cggctgcgcg gtgacggtct
     7621 tcaatgtaaa tgcccagcga ttcgcacagg cggccgaggc tgtaagaaat tctccgcggg
     7681 agcagcttgc ggctcatctt cacggtgcag agcttttccc gtttccactg gtaaccgagc
     7741 gagcggaatt ccgcctggat aaaaccgtaa tcgaaattca cattatgcgc gacgaaaacc
     7801 tttccttccg tcatctcgat gatcctccgc gccacttccc agaatttcgg agcatcacgg
     7861 accatctcat ccgaaatgcg cgtcatgcgc gtgatgtatt cgggaattct ccgttcggga
     7921 ttgataagcg tggagaattt gtccacgact gtgagcccgt catgaatgag aatgcagatc
     7981 tccgtgatac ggtcgcgttc cgggcttccg ccggtggttt ctatgtcgac gatggcgaac
     8041 aacttgttcc tgctgggaac tgcaaatata cagccggctg cggcgcattt cttccctgtg
     8101 cggctgtgtt aatttaccgc cacaacccgt ggagtcaaag gttccgggac cacggcggcc
     8161 ggggctgtcc gcacagcatt ccggactatt ttctctactt ttggccatca aattcttcac
     8221 tgtggcgaaa cattccagaa aagagctcaa acgtattttc gacagttttg aaaaactccg
     8281 cgtgctgatc atcggcgatg tgatgatcga caattatgta tggggaaaag tatcgcgcat
     8341 ctctcccgaa gcgccggtcc cggtggtttc cgtggcgcgg aaagagcagc gtctcggcgg
     8401 tgcggccaac gtggcgctga acgtgcagtc gctgggagcg acgcccatcc tctgctccgt
     8461 catcggcgtc gaccgcaacg ggcagctttt cctcgagctg atgcagcagg aaaaactcgc
     8521 gccgaagggc atcctgaaaa gccgcgaccg catcactacg gaaaaaacgc gcatcatcgg
     8581 gaacaaccac cacctgcttc gcgtggatga agagatcgat cacgacatta caccgcacga
     8641 aacaggactt ttcctcgagc gtatcgcata cctgctcgac acgcagaaga tagacgtgat
     8701 cattttcgaa gattacgaca aagggctgat cacggaaaag ctgatccgcg aagtcgtttc
     8761 acgcgcgcgc aagctgaaag ttcccgtggt cgtcgatccg aagaaaaaac atttcatgaa
     8821 ttaccagggc gcgacgctgt tcaagcccaa cctgaaagag ctgaaagaag gcctgaaagt
     8881 ggatttcgac cagagcagcg caaaagaatt gtcgaaagcc gtcgacctgc tcaaaacaaa
     8941 gctgaagatc gatatggcgc tgatcaccct ttcagaacat ggcatttatg ttcacgggcg
     9001 ccacgtgaaa aagcttcttc ccgcacacgt gcgcaacatc gcagatgttt caggcgcagg
     9061 cgataccgtg gtcagcgtgg ctgcgctgtg tcttgccgcc ggactgaaac ccgagctgct
     9121 cgcggagatc tccaacattg caggcgggct tgtctgtgaa tcggtgggcg tttccccgat
     9181 cgataaagaa cagctgctgg aagaaagcgt gcagcttatc gccggaaaaa attaacggca
     9241 cggtttctgc cgcaggaaaa acatgcgcat cctccctttt ttcgttccgg ttttcgcgat
     9301 cgctcttctc gccagctcat tctgcgggaa gaagaagaaa aagaaacagg aattcaccgt
     9361 gaacggaacc gtgctgctga cgcacagcta ttgcggagga attgttccgc ccgctgaatt
     9421 tctcgaagcg atgaaaaccc cgcaaccggc tcccggcttt cacctctttg taaaaaaagg
     9481 gaacacgaac gtttcgggca gcgccatcct cgatacgatc gtcaccgatg cgcagggaca
     9541 tttctccgtt tcacttccgg cgggaacgta ttgcatcgtc aaagactgga aggccccgaa
     9601 gtattccgtt ccgcaggaca accagtacgc agaatacgac acagcctgtt atcgcaaaga
     9661 atacgcgcag tgtgattacc tgctcaacct ttccgcgaac gtggacagcc tcaagctcgt
     9721 tttccacgag ggctgcagct gggaacagcc ctgcatgaat taccacggac ctttaccgcc
     9781 ggcagcgcac ccgggaggac atccgcataa tgagtagtga gatggagaac agtgaggtat
     9841 tattttccgg actcaggacg cctgccatta tggaactggt gtccgccaaa agaaaaaaca
     9901 ccgggtttcg aaacgctttc aacttcactg gaacagcaca cggtattccc gcttcatatc
     9961 tcattactgt tcatcagctt ccacagctca tctttcagct cctgtattcc tttccctgtt
    10021 gcggaggaaa aaataacgta aggtgttttg aattttttca ggaacctttt atcgagatct
    10081 tttttcgtcg cggtgaataa ttcctcatcg agcagatcgg ctttggatac gccaagcaat
    10141 ctttttttgt gaagcaattc aggattatat tcctgcagct cgtgcataag cacctggtaa
    10201 tcttttacaa tatccgtgct gtcggcagga acaagaaaca gcaggctgga attccgttcg
    10261 atgtgacgca ggaagcgcag accaagtccg cggccttcat gcgcaccttc gatgatgccg
    10321 ggaatatccg ccatgatgaa cgagcggtcg tcgcggtact tcacgatgcc gaggttcgga
    10381 acgagcgtcg tgaacgggta atcggcgatc tcgggcctgg ctgcggaaac aaccgacagc
    10441 agcgttgatt tcccggcgtt gggaaaaccg accagcccga catccgcgag cagtttcagc
    10501 tcgaggactt tccattcttc tcttccgggt tcaccgggtt gcgcaaagcg cggcgtctgc
    10561 cgcgtggcgg ttttgaaaaa atcatttccc tttccacctc ttccgccggg cacgataata
    10621 cgttcttcgc cgtcttcggt aatttcaaaa atagtctcgc cggtctccgc atctttcgcc
    10681 acagtaccga gcggaacttc gatcacttca tctttcccgt cggcgccggt catgttcgct
    10741 cttccgcccg attcaccgtc ttcggcgatc acgtgcttgc ggtatttgag gtgaagcagc
    10801 gtccacagct gcttgttccc gcgtacgatg acgtgtcccc cgcgtccgcc gtcgccgccg
    10861 tcgggtccgc ctttcgctgt ttgcgccgtg cggagaaaat gcacggagcc tcctccgccc
    10921 ttcccggagc ggcagcagat cttgacgtaa tcgacaaagt tggattcgga cacttcagtt
    10981 cggagttcgg agttcggaag cttccgaacc gtgattagca atattatcgc aggtcatcga
    11041 cttccttgca aagctttgca aagatctctt cgatatcacc gatgccgtat acggaacggt
    11101 atttaccctg cgccgcgtaa tagtcgcgca gcggagcagt tttcgtattg tattccgtga
    11161 tccttttttc aacgatctgc ggatcctggt catcggcgcg gccggaatct tttccgcgga
    11221 gaaggatcct ttttttcagc tcctcatcat tcacttccag cgcgagcatg gctgagacga
    11281 tcattccctt gccggcaaga aatttatcga gcgcttccgc ctgtgcgatc gtgcgcggga
    11341 agccgtcgaa aataaacccg acctggttca tgtacatatt cacttccgcc tcgagcacac
    11401 ggatcgtcag ctcatccgga acaagcattc ctttgtccat atagctgatc acttctttcg
    11461 cgaggggaga atcacttttc ttgatcgcgc ggaaaatatc gccggtggaa agctgcttga
    11521 acccgtattt gtcgacgagc ttcgtggcct gggttccttt tcctgcgccc ggaggacctg
    11581 agagaacaat gtttatcata ctgatcgtgc tgaagttgtt tattgattcc tgatcacgta
    11641 aatgtcgcgg aggttccggc cgaggccatt gtagtcgaga ccatagccca cgataaaatc
    11701 gttcgggatg cggaggccga cataatcgac cgggatctgt tttttataca cttccggctt
    11761 cagcagcagc gacgcgatgc ggagcgacgc cggttcccgc ttcagcaact gctccaccag
    11821 ctgcgcgatc gtattgccgg tatcgacaat atcctccacg atgatcacat cgcggccggc
    11881 gatcgattca ttaaggccga tgagctcctt cacggttcct gatgaagacg ttccttcata
    11941 cgaagccagc ttgacgaaag aaacttcgca gttcagcttc acggaacgca cgagatcagc
    12001 agcaaaaagg aaagaaccgt tcagcacgac gaggaacaac ggatgctttc cgtcgtaatc
    12061 catgttgatc gcgttcgcta cgttcttcac cgcggcgctg atcttttcct cactgatata
    12121 gggaacaaaa tgcttatcgc ctaactgaat cgggtccatg atttaaggtg tgcaaaatta
    12181 acgtttattt cagcgcctgc gcttcaagac tatacaaaac aaaatccgtt gccgcacctg
    12241 gtttgcgcag aaaaagtcct gcctgttgat aattagatat gacgaaacgc taacatttcc
    12301 tgttgaaaac gcgtttattt atatgtagaa aaaaaagcgt cggaggggaa tcatattttt
    12361 ctacttttgg aaccctttcc ccggaaaatt acaacaggcc cgatccagca atgagtactg
    12421 aagaaaaaaa gaacacgacg cgccgacctg cccatctttc gcaggtaact cagcag
//
LOCUS       DASTSF010000367         4355 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1046677,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000367 DASTSF010000000
VERSION     DASTSF010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4355
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1046677"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..627)
                     /locus_tag="VFU15_10505"
     CDS             complement(<1..627)
                     /locus_tag="VFU15_10505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012780580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718258.1"
                     /translation="MAKRRKEKTYSLTAFAWQRLRRNKLALSGIGIIAFSILISILGY
                     LITPDKSPDADTHIDEIGIKRPGFSCNLIRLRKNTDINNQSFIRTMLFGRVNDYEYHA
                     YKNYWFSGNDILWEEYNDGPGIPKIWRYNLADAIYPINPAFQIKNDTINGVMDFYAYG
                     ETRKMHKTVQELRQEILGNIYHEKFLLGTDHFGRDLLSRLMIGTRISLF"
     gene            complement(632..1753)
                     /locus_tag="VFU15_10510"
     CDS             complement(632..1753)
                     /locus_tag="VFU15_10510"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU4718259.1"
                     /translation="MTLLIYIVVGIALSYGIFLLYTGFYFFVPTVKPEHPAKPGKISV
                     VIAFRNEKVHFSECLYALSNIDFNSENAELILVDDHSDDGSRAEAADFCRRHAYARLL
                     LLEAEEQGKKAALHKGIREADGEIIAVTDADCIAGKNWLKVIRERFTDPEKNFLTGPV
                     KFVSHDSFVEKLQASELCVLQALTGGSVKAGFPLFANGANMAFRKKFYEESDGMRDSA
                     SASGDDMFLLIEAVKHDPRSVGYLDNVSATVLTWPEKTWKGMLEQRTRWASKTGKYRR
                     PLLTITALLVFLMNASPLLLTLLSLVERDLLVAAVVTWFWKASVDLLLLSLAVPFFKE
                     RSLAASAVAGTFIYPFIGIMIVPGIMRRSFTWKGRKWKA"
     gene            complement(1750..2697)
                     /locus_tag="VFU15_10515"
     CDS             complement(1750..2697)
                     /locus_tag="VFU15_10515"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HEU4718260.1"
                     /translation="MPLNSSLAKRISLLIKILIVLVAAAAIFLKFSKASMAETIGQAF
                     RTGISRNLSLFIFAFLLIPVNWALEALKWQKLVSVSEKITFMQAFGSVLSGLTVGTLT
                     PNRFGEFAGRIFLLSKTGKLEAVWLSFIASAAQVIVTLLAGLPGFIILCTDGNFLPGG
                     FRKNISYEAFAFALLLAGLVLVYFFLPDAARNRLRKLRTVPRHLFASALFLSAARYAV
                     YAFQFFLLLRMMGVEGHSGSIFAGITVNYFLVTLTPALPVMEIFVRAYLAGTVLGSVS
                     GQAELCGITAALIWIMNIAVPSVAGMGWVFAWRIFRKKT"
     gene            2725..3303
                     /gene="ruvC"
                     /locus_tag="VFU15_10520"
     CDS             2725..3303
                     /gene="ruvC"
                     /locus_tag="VFU15_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008237467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEU4718261.1"
                     /translation="MHSPKTQQEKIILGIDPGTNVMGYGVISVSGQKMKLIDLGVVRM
                     EKNADHTLKLKKVFESTVELLEKHLPDEFAVEAPFFGKNVQSMLKLGRAQGVAIAAAL
                     TRDIPIFEYSPKKIKLSITGNGNASKEQVGMMLQRLLVFRELPEYLDATDGLAAAVCH
                     FFQGKKESSPGKKHASWNSFISENPGRVQVKK"
     gene            complement(3307..3708)
                     /locus_tag="VFU15_10525"
     CDS             complement(3307..3708)
                     /locus_tag="VFU15_10525"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015029322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEU4718262.1"
                     /translation="MASIFSRIVAGEIPCHKVAETESCLAFLDVFPLVTGHVLVIPKK
                     EIDYIFDLDDELFSDLHLFSGKVAKALKKAIPCRRVGVAVIGLEVPHAHIHLVPMNVM
                     SDINFSREKMKPSKEELESTAKKIRDAYEGL"
     gene            complement(3708..4181)
                     /gene="greA"
                     /locus_tag="VFU15_10530"
     CDS             complement(3708..4181)
                     /gene="greA"
                     /locus_tag="VFU15_10530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014681465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEU4718263.1"
                     /translation="MSQINYVTEEGLKKLQDELNHLKTKERPSISRQIAEARDKGDLS
                     ENAEYDAAKDAQGLLELKISKLEELISNSRIVDESQLDTSKVLILCKVTIRNVKTNQK
                     MTYMLVPENESDLKSGKISVNSPIAKGLLGRKKGEIADVVVPSGAMKFEILDIAR"
BASE COUNT         1096 a         1095 c         1120 g         1044 t
ORIGIN      
        1 gaaaagcgat attctcgttc cgatcatcag gcggctcagc aggtcgcggc cgaaatggtc
       61 ggtgccgagc aggaattttt catggtaaat gttgcccagg atttcctgcc gcagctcctg
      121 cacggtcttg tgcatcttgc gtgtttcgcc gtaagcgtaa aaatccatca cgccgttgat
      181 cgtgtcgttc ttgatctgga atgccgggtt gatcgggtag atcgcatcgg caagattgta
      241 cctccagatc ttgggaatgc cgggaccgtc gttgtactct tcccacagga tgtcgtttcc
      301 gctgaaccag tagttcttgt acgcgtggta ttcataatcg ttcacacgcc cgaacagcat
      361 tgtccggata aagctctggt tgttgatgtc cgtgtttttc cgcaggcgga tgagattgca
      421 gctgaatccc ggccgtttga tgccgatctc gtcgatgtgt gtgtctgcgt ccggactttt
      481 atcaggtgtg ataagatagc cgagaatgga aatgagaatg gaaaaagcga tgatgccgat
      541 gcccgaaagc gccagcttgt tcctgcgcag gcgttgccac gcaaatgcgg tcagtgagta
      601 ggtcttttct ttcctgcgct tggccattca atcaggcttt ccattttctg cccttccacg
      661 tgaagctcct gcgcatgatt ccgggcacga tcattattcc aatgaaggga tatataaatg
      721 tgcctgcaac cgcggaagct gccaacgaac gctccttaaa aaatgggacg gctaaagata
      781 acaacaaaag gtcaactgaa gctttccaga accacgtgac gaccgcggcc acgagcaggt
      841 ctctttccac gagtgaaagc agggtgagca gcaggggaga agcgttcatc aggaaaacga
      901 gcagggcggt tatcgtcagc aggggcctgc ggtattttcc cgtttttgaa gcccaccggg
      961 ttcgctgctc cagcattcct ttccatgttt tttccggcca cgtgagtacg gttgccgaaa
     1021 cgttatcgag gtaacctacg ctgcgcggat cgtgctttac ggcttcgatc agcaggaaca
     1081 tgtcgtcacc gctcgccgat gcagaatcac gcatgccgtc cgattcttcg tagaattttt
     1141 tccggaaagc catgttcgcg ccgttggcga acaatggaaa cccggctttc accgagccgc
     1201 ccgtcaatgc ctgcagcacg cacagttccg atgcctgcag cttttccacg aagctgtcgt
     1261 gcgaaacgaa tttgaccgga cccgtcagga aatttttttc aggatcggtg aagcgttcgc
     1321 gaattacctt cagccagttt tttccggcta tgcaatcggc gtccgtcacc gcaatgatct
     1381 ctccgtccgc ctcccggatt cctttatgca gcgccgcctt ttttccctgt tcttccgctt
     1441 ccagcaaaag tagcctggcg tacgcatgcc tgcggcagaa atccgctgct tcggcccggc
     1501 tgccgtcatc tgaatgatca tcgacgagga tcagctctgc attttcagaa ttgaaatcga
     1561 tattggacag cgcgtaaaga cattcggaaa aatgtacttt ttcattgcgg aatgcgatca
     1621 caaccgaaat ttttcctggc tttgcaggat gttcgggttt taccgttgga acgaaaaaat
     1681 aaaaaccggt gtacagcaaa aaaataccgt acgaaagcgc gatgcccaca actatgtaga
     1741 taagcagggt catgtttttt ttctgaagat cctccacgca aacacccaac ccattccggc
     1801 gacggaagga acggcgatgt tcattatcca gataagcgcc gccgtaattc cgcataattc
     1861 agcctgcccg gaaacggatc cgagaactgt tccggcgaga taggcccgga cgaaaatttc
     1921 cattaccgga agtgcgggtg taagcgtaac gaggaaatag ttcacggtga tgccggcaaa
     1981 gatagacccg gaatgtcctt cgacgcccat catcctgagc agcaaaaaaa actggaatgc
     2041 ataaacggcg tagcgtgccg cagaaaggaa aagtgcggaa gcaaaaagat ggcgcggaac
     2101 agtgcggagc ttccggagcc tgttccgcgc tgcatccgga aggaaaaaat aaaccaggac
     2161 caggccggcc agcagcaacg cgaatgcgaa cgcctcgtaa gaaatatttt tccggaaccc
     2221 gcccggaagg aaatttccgt ctgtgcaaag aataataaaa cccggcagac ctgccagcag
     2281 tgtgacgatc acctgtgcag cgctggcaat gaaagaaagc catacagctt ccagtttccc
     2341 ggttttggaa aggaggaaga tacgtcctgc aaattccccg aaccggttcg gcgtaagcgt
     2401 acccacggta agtccgctga gcacgctgcc gaatgcctgc atgaaagtta tcttttccga
     2461 aacagaaacc agcttttgcc acttcagcgc ttcgagcgcc cagttgacgg gtatcagcag
     2521 gaaagcgaaa atgaaaagtg ataaattccg gctgatcccg gtgcggaagg cctgaccgat
     2581 ggtctccgcc atggaggctt ttgaaaactt aaggaagatc gccgccgccg ccacaagcac
     2641 aataagtatc tttatgagta ggctgatccg tttggcaaga gaagaattaa gcggcatcag
     2701 ggtaaattta cgataagctc cgaagtgcat tctccgaaaa cacagcagga aaaaattatt
     2761 ctcggcatcg acccgggaac gaacgtgatg ggatacggcg ttatctctgt gtcgggtcag
     2821 aaaatgaagc tcattgatct cggtgtcgtt cgcatggaaa aaaatgcaga tcacaccctc
     2881 aagctcaaaa aggttttcga aagcaccgta gaactgctgg aaaaacatct tcccgacgag
     2941 ttcgcggtgg aagcgccttt tttcggaaag aatgtgcaat ctatgctgaa gcttggccgc
     3001 gcccagggtg ttgcgattgc agccgcgctt acgcgtgata tcccgatttt cgaatacagt
     3061 ccgaagaaga tcaagctttc cattacgggc aatggcaacg cttcgaaaga gcaggtcggg
     3121 atgatgctgc agcgcctgct tgtgttccgc gagttacccg aatacctcga tgcaacggat
     3181 ggacttgccg ccgcggtatg ccattttttc cagggaaaaa aggaaagttc cccggggaaa
     3241 aagcatgcat cctggaattc gttcatttcc gaaaacccgg gcagggtaca ggtcaagaaa
     3301 taggcattac agcccttcgt aagcgtctcg tattttcttc gccgtcgatt ccagctcttc
     3361 tttcgaaggt ttcatctttt ccctgctgaa attgatatcg gacatgacgt tcatcgggac
     3421 aagatgaatg tgcgcatgcg gcacttcaag cccgataacg gcgacaccta cgcgacggca
     3481 gggaatggct tttttcagcg ctttcgctac tttcccggag aagagatgaa ggtcggaaaa
     3541 cagctcatcg tcaagatcga aaatgtaatc gatctccttt ttaggaatca caagcacatg
     3601 gccggtaacc agcgggaaaa cgtccaggaa ggcaagacag ctttctgttt ctgccacttt
     3661 atggcaggga atttcgccgg cgactatacg tgaaaagata ctggccatta ccgggcaata
     3721 tcgagtattt cgaatttcat cgcgcccgaa ggaaccacca cgtccgcgat ctcgcccttc
     3781 ttccggccga gaagaccttt tgctattggc gaattgacgg atattttccc ggatttcagg
     3841 tcggattcat tctccgggac aagcatatag gtcattttct ggttggtctt cacatttcgt
     3901 attgtgacct tacagagaat gagcactttg gatgtatcga gctgggactc atccacgatt
     3961 cttgaattgc tgatcagttc ctcgagcttg gagatcttca gctcaagaag gccctgggcg
     4021 tctttggcag cgtcatattc cgcgttttcg gataaatcac ctttgtcccg ggcttctgca
     4081 atctggcggg atatgctcgg tctctccttg gttttaaggt ggttaagctc atcctgaagc
     4141 ttctttaagc cttcttcagt tacataattt atctgagaca tggttagaaa attaaaattt
     4201 aacagcggtt gaaaacagaa ggagaatccc gggggtttcc cgggactctc ctgtaaatca
     4261 aagttaagaa atttagccga aacggccaat aatcagagat taagccttgc cccgaagctg
     4321 tgaacaccct ggaaaggatc agtcaggcga tagga
//
LOCUS       DASTSF010000368         2594 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1586470,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000368 DASTSF010000000
VERSION     DASTSF010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2594
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1586470"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..881)
                     /locus_tag="VFU15_10535"
     CDS             complement(<1..881)
                     /locus_tag="VFU15_10535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718264.1"
                     /translation="MSSLSAIPRFIPAFPAPAQLSRKKGGVFLTDNRAAAAQRKISEP
                     RGNAAVAQLHATAAADKIFPAEQPVQLARNKRIRGTVGGALGLGVTGALIGSVVPGIG
                     TTLGGLVGLGVGALLGYGLSGPTIYPNLHRNEYQNELRDYRRYQRERQGARRAYEQGT
                     IIRYSNSNSRAFTRLLHQGQVLYTYDVSNQLAIGSNAGPIKHAIVAGDQDVKAAGMAN
                     VILPTQGQQVTSEYEQAAARVEHLNLLREEFEERAKEIIKRRNVQRHTVDNIPNEKEK
                     DIVREYFQTMSQLEHYE"
     gene            complement(986..2365)
                     /locus_tag="VFU15_10540"
     CDS             complement(986..2365)
                     /locus_tag="VFU15_10540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718265.1"
                     /translation="MKNTHRKFSFLLLASALFAAGCGTETPPKNMMTSQAPPNSTTWA
                     ISGYTVTVNGKAFFGNGVSYSPIPWGTCSAFNPYGDFTINTWSSVWQRDLTLMRANNV
                     NLIKTYNTLDSVQLVNAGDPYTWDHDHSHFLDSCWNNGNNPVYVLMGYAPPKNQSSIF
                     FSDTWSDPANVAARAVIKSSLVKLAKKWGSYPAVMGFVMANEINASNIINSSQFFTYW
                     NNVALAMDSAAPGKLVTLANVDDGMNTVNAGNPYMTSPNFFWGYNSYRGNWTNSNGFD
                     NMFSAFATATASNKHPLMLTEWGAPACAQDTGGAIINMSASQMQDLVTYVTGHYGNMV
                     ANRSDDSTGVCCGGAYFEWTDEWWKADPAGMQCNQPNAAPACHTGIWDASGTPWLPNF
                     PGHYWDEEGFGLFSITPVSPSTRVPVVSGGCIGPWNPKTNQPYPADSLGMRPHATALF
                     SAMKNSMAH"
     gene            complement(2413..>2594)
                     /locus_tag="VFU15_10545"
     CDS             complement(2413..>2594)
                     /locus_tag="VFU15_10545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718266.1"
                     /translation="QFLIPELKKKFPEYTHEVHYSFNDSTGSVSKWKGGEKSQTEQYD
                     TNDELNRYNESGQNH"
BASE COUNT          482 a          679 c          766 g          667 t
ORIGIN      
        1 tcttcgtaat gctccagctg gctcatggtc tggaaatatt ctctcacgat atctttttct
       61 ttttcattcg gaatattgtc caccgtgtgg cgctgcacat ttcttctctt gataatctcc
      121 tttgcccgct cttcgaattc ctcgcgcagc aagttcaggt gctccacccg tgcagcggcc
      181 tgttcgtatt ctgatgtcac ctgttgtcct tgtgtcggaa ggatgacatt cgccattcct
      241 gctgccttca cgtcctggtc gccggcgacg atcgcatgct tgatgggacc ggcgttgctg
      301 ccgatcgcga gctggttgct tacgtcatag gtatacagca cttgtccctg gtgaagcagg
      361 cgtgtaaatg ccctgctgtt ggaattcgaa tagcggatga tcgtgccctg ctcgtaagct
      421 cttcgggcgc cttgtctttc acgctggtat ctcctgtaat cgcgcagctc gttttggtat
      481 tcattcctgt gcaggttggg ataaatggtg gggccggaga ggccgtaacc gagaagtgcg
      541 ccgacgccca ggccgaccag cccgccgagc gtggtgccga ttccaggaac aacgctcccg
      601 atgagggcgc cggtaacacc gaggcccaat gcgccaccga cggttccccg tatcctttta
      661 tttcgggcaa gctgcacagg ttgttccgcc ggaaagattt tatccgcagc tgcggttgcg
      721 tgcaattgtg caaccgccgc attcccgcgg ggctcactga tcttccgctg cgcagcggct
      781 gcgcggttgt cggtaagaaa aacgcctcct ttttttcgcg acagctgggc cggtgccggg
      841 aaagccggaa tgaatcttgg tatggcggat agcgaagaca tgtatggcct tgtttttttg
      901 agtaatgatc gccgggagtc ggccgggctg catggtctaa agcgggccgg gcagttccat
      961 gaatgatttc cggccaggca taagcctaat gcgccatgct gtttttcatc gcactgaaaa
     1021 gagcggtggc atggggacgc atcccgagac tgtctgcagg atacggctgg ttcgttttcg
     1081 gattccacgg gccgatgcag ccgccgctga ccacagggac gcgcgtggaa ggacttaccg
     1141 gtgtaatcga aaacaatccg aatccttctt cgtcccagta atggcccggg aagttgggca
     1201 gccacggtgt tcccgatgcg tcccagatgc cggtgtgaca ggctggcgcg gcattgggct
     1261 ggttgcactg catgcctgcc ggatcggcct tccaccattc gtcggtccat tcgaaatacg
     1321 caccgccgca gcacacgccg gtagaatcat ccgaccggtt cgcgaccatg ttgccgtaat
     1381 gaccggtaac ataagtgacg agatcctgca tttgcgatgc actcatgttg atgatcgctc
     1441 cgcccgtgtc ctgtgcacac gcaggtgcgc cccattccgt gagcatgagc gggtgcttgt
     1501 tcgacgccgt ggccgttgcg aacgccgaga acatattgtc gaagccgttc gagtttgtcc
     1561 agttgccgcg gtaggaattg tatccccaga agaagttggg cgatgtcatg tacgggttgc
     1621 ccgcgttcac ggtgttcatc ccgtcgtcga cgttggcgag cgttaccagc ttcccgggcg
     1681 ctgcactgtc catggcgagc gcgacattgt tccagtatgt gaagaactgc gaactgttga
     1741 tgatgttgct cgcattgatc tcgttcgcca tcacgaatcc catcacggcc gggtagcttc
     1801 cccatttttt cgccagcttg acgaggctcg atttgatcac ggcgcgcgcg gcaacgtttg
     1861 ccggatcgct ccacgtgtcc gaaaagaaga tggacgattg gtttttcgga ggagcataac
     1921 ccatgagcac gtacaccgga ttgttcccgt tgttccagca tgaatcgagg aaatggctgt
     1981 ggtcatgatc ccatgtgtat ggatcacctg cattgacgag ctggacggaa tcgagcgtgt
     2041 tgtaggtttt gatgaggttg acgttgttgg cccgcatcag cgtgaggtcg cgctgccaga
     2101 cggagctcca tgtattgatc gtgaaatcgc cgtatgggtt gaaggccgag caggttcccc
     2161 acgggatcgg ggaataactg acgccgttgc cgaaaaatgc tttgccattc acggtgaccg
     2221 tgtacccgct gatcgcccag gtggtggaat tcggcggagc ctgtgaagtc atcatgtttt
     2281 tcggaggcgt ttcggtgccg catcctgcag cgaaaagcgc ggaggcgagc aggaggaaag
     2341 aaaatttccg gtgagtgttt ttcatggtga tggtgattaa ggtgtagttt atcctggtga
     2401 ttgatgtttg gttcaatggt tctgcccgga ttcgttgtaa cggttgagct cgtcatttgt
     2461 atcatattgt tccgtctgcg atttttcacc gcctttccat ttcgataccg agccggtgct
     2521 gtcgttgaaa gaataatgga cctcatgggt gtattcgggg aatttttttt tcagctcggg
     2581 gatgagaaac tgct
//
LOCUS       DASTSF010000369         3468 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1721700,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000369 DASTSF010000000
VERSION     DASTSF010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3468
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1721700"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(18..1040)
                     /locus_tag="VFU15_10550"
     CDS             complement(18..1040)
                     /locus_tag="VFU15_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEU4718267.1"
                     /translation="MGVFDLFTQEIAIDLGTANTIIIHNDKVVVDEPSIVAVDRATGK
                     VIAVGKQAMQMHGKTHENIKTIRPLKDGVIADFHAAEHMIRGMIKMINPGRRVFQPSL
                     RMVICIPSGITEVEKRAVRDSAEHAHAKEVYLIHEPMAAAIGIGIDVEEPMGNMIIDI
                     GGGTSEIAVIALGGIVCDKSIRIAGDEFTGSIEEYMRRQHNILIGERTAERIKIEVGA
                     AMAEIENPPPDFPVHGRDLMTGIPKEIYVSYVEIAHALDKSISKIEEAILNALEMTPP
                     ELSADIYRTGIYLAGGGSLLRGLDKRISLKTKLPVHVAEDPLRAVARGTGIALKNIDK
                     FPFLIK"
     gene            complement(1147..2673)
                     /gene="purH"
                     /locus_tag="VFU15_10555"
     CDS             complement(1147..2673)
                     /gene="purH"
                     /locus_tag="VFU15_10555"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEU4718268.1"
                     /translation="MSRPVKIKNALISVFYKDNLEPVIRRLHELGVGLFSTGGTQTFI
                     EQLGIPVTPVEQLTSYPSILGGRVKTLHPKVFGGILGRRGLDTDVAQLEEYEIPEIDL
                     VIVDLYPFGETVASGASEQDIIEKIDIGGISLIRAAAKNFSDVLIVASRQDYPGLLDI
                     LNSSNGSTEVEDRRRFAARAFAVSSEYDTTIFNWFNSDGAELAFRSAWNGGRTLRYGE
                     NPHQSAKFFGDLSQMFDQLCGKELSYNNLLDIDAAVNLMADFTDTTFAILKHNNACGL
                     ASRKTVLQAWTDALAGDPVSAFGGILICNRIIDAKAAEEITKIFFEVIIAPGYEPAAL
                     ETLRQKQNRIVLVQKPVTLPSGQFRSLLNGVIAQEKDLRSETAADMKPVTHSVPTEDE
                     YADLVFANKLVKHTKSNTIVLVKNRQLLASGTGQTSRVDALKQAIAKTQQFGFSLDGA
                     VMASDAFFPFADCVEIANGVGIHAVVQPGGSIRDKDSVEFCDRNGMAMVFTGTRHFKH
                     "
     gene            2717..2950
                     /locus_tag="VFU15_10560"
     CDS             2717..2950
                     /locus_tag="VFU15_10560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718269.1"
                     /translation="MKIPGKTLFFRFVEKMGTLLKTAIASVKKSPAKMSAGREENCLS
                     GQLSAFLFLPSHPAVIWNSAPVEVAFVVYNPRT"
     gene            2947..>3468
                     /locus_tag="VFU15_10565"
     CDS             2947..>3468
                     /locus_tag="VFU15_10565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718270.1"
                     /translation="MKKLLLHTLLLSVSVLPAQVVTFTNEYAIWGESHCAHNLRQTID
                     GGYIFTADAVPEMDTAVNPLAYAYLVKIGAGGATQWIKSFPKSDFFIKPFDANCVCAA
                     SDGGYLMATCFYSSQYPASSNNPARILLVKTDAAGNQLWSYIYPGLGLSACYCVNETT
                     DHGFIVCGETADSV"
BASE COUNT          708 a          932 c          996 g          832 t
ORIGIN      
        1 ggtattccgg atttccccta cttgatgagg aacggaaatt tgtcaatgtt cttgagcgcg
       61 atgcccgttc cgcgggctac tgcgcggagc ggatcttccg ccacgtgaac aggcagcttc
      121 gttttgagcg aaatgcgctt gtcgaggccg cggaggagag aacctccgcc ggcaaggtag
      181 atgccggtac ggtaaatatc ggcggacaat tccggcggcg tcatctcgag cgcattgaga
      241 atggcttcct cgatcttcga gatcgatttg tccagcgcat gcgcgatctc gacataggaa
      301 acgtagatct ccttcggaat acccgtcatg agatcgcgcc cgtgcacggg aaaatccggc
      361 gggggatttt cgatctccgc catggcggcg ccgacttcga tcttgatgcg ttccgctgta
      421 cgttcaccga tgaggatgtt gtgctggcgg cgcatgtatt cttcgatgct gcctgtaaac
      481 tcgtcacccg cgatgcggat ggatttgtcg cagacgatac cgccgagcgc gatgaccgca
      541 atttccgacg taccgccgcc gatatcgatg atcatgttgc ccatcggctc ctcgacgtcg
      601 atgccgatac cgatcgcggc cgccatcggc tcatggatga ggtaaacttc tttcgcgtgg
      661 gcgtgctccg ccgaatcgcg caccgcgcgc ttttcaactt cagtgatgcc ggaaggaatg
      721 cagatcacca tgcgcagcga aggctggaac acgcggcgcc cggggttgat catcttgatc
      781 attccgcgga tcatgtgctc ggccgcgtgg aaatcggcga tcacgccatc cttcagcggg
      841 cggatcgttt tgatgttctc gtgcgttttg ccgtgcatct gcatggcctg cttgccgacg
      901 gcgatcactt ttcccgtggc cctgtcgacg gcaacgatgg aaggctcgtc cacgaccact
      961 ttatcgttgt gaatgatgat cgtattggca gtgccaagat cgatggcaat ttcctgagta
     1021 aagaggtcga atactcccat aacggttgaa attccaaagt ttcaggttcc aaattccagg
     1081 ttttccgggt tggcgattgc cgttcaggat gggatttgga atttcatttt tgggtttttg
     1141 ttagtattaa tgcttgaaat gtcttgttcc ggtgaacacc atcgccatcc cgttcctgtc
     1201 gcagaattcg acagaatcct tgtcgcgtat cgatccgccg ggctgcacaa ccgcatgaat
     1261 tcccacgccg ttcgcaattt ccacgcaatc cgcgaaaggg aagaacgcat cggaagccat
     1321 caccgcgccg tccagtgaaa aaccgaactg ctgcgttttc gcgatcgcct gcttgagcgc
     1381 atccacgcgt gatgtctgtc ccgttccgct ggccagcagc tggcggtttt ttacaagaac
     1441 gatggtgttc gacttggtat gcttgaccag cttgttcgca aaaacaagat cggcatattc
     1501 atcttccgtg ggaaccgaat gtgtgacggg tttcatgtcc gctgcagttt ccgagcgcag
     1561 gtctttctcc tgtgcgatga ccccgttgag cagggaacgg aactggccgg acggaagcgt
     1621 caccggtttt tgtacgagta cgatgcggtt ttgtttctgc cggagcgttt cgagcgctgc
     1681 gggttcgtag ccgggagcga tgatcacctc gaagaagatc ttcgtgatct cttccgccgc
     1741 cttcgcgtcg atgatgcggt tgcagatgag aattccgccg aatgcggaaa caggatcgcc
     1801 ggcgagcgcg tcggtccagg cctgcagcac ggttttgcgc gaggcgagtc cgcaggcgtt
     1861 gttgtgcttg agaatggcga acgtggtatc ggtgaaatcc gccatgagat tcacagcggc
     1921 gtcgatgtcg agcaggttgt tgtacgaaag ctctttcccg cagagctggt cgaacatctg
     1981 cgaaagatcc ccgaaaaatt tcgccgactg gtgcgggttc tccccgtagc gcagggtgcg
     2041 gccgccgttc catgcgctgc ggaacgcgag ctcggctccg tcgctgttga accagttgaa
     2101 gatcgtggtg tcgtattccg aagaaacggc gaatgcgcgc gccgcgaagc gacggcggtc
     2161 ttccacttct gtcgaaccgt tactgctgtt aaggatatcg agcaggccgg gataatcctg
     2221 gcgggaagca acgatgagca cgtcgctgaa attttttgca gcggcgcgga tgagcgagat
     2281 gccgccgatg tcgatcttct cgatgatgtc ctgctccgaa gcgccggatg cgaccgtttc
     2341 cccgaaggga taaaggtcga cgataacgag atcgatctcg ggaatttcat attcttccag
     2401 ctgcgcgacg tccgtgtcga gtccgcggcg gccgagaatt ccgccgaaca ctttcgggtg
     2461 aagcgttttc acgcgcccgc caagaatgga aggataggaa gtcagctgct cgacgggagt
     2521 aacgggaatg ccgagctgtt cgatgaaggt ctgcgtgccg ccggtgctga aaagtccgac
     2581 accgagctca tgcaggcgac ggatgacggg ttcgagattg tctttgtaaa atacggaaat
     2641 cagtgcgttt ttgatcttga ccggacggct cataatgttt cgggaaaaag tgcttcgaag
     2701 gtacatcatt tagcgcgtga agattccggg caaaaccctg ttttttcgct ttgttgaaaa
     2761 gatggggacg ttgttgaaaa cagcaattgc atccgtcaaa aaatcacctg caaaaatgag
     2821 cgctggccgg gaagaaaatt gtctttccgg acaactgtcc gcatttcttt tcctgccgtc
     2881 gcatccggct gttatttgga attcagcgcc tgttgaagta gctttcgttg tatacaaccc
     2941 tcgcacatga aaaaactgct gcttcatacc ctgcttcttt ccgtgtccgt tcttccggcg
     3001 caggtcgtca cgttcaccaa cgaatacgcg atctggggcg agtcgcattg tgcgcataat
     3061 ctccggcaga cgatcgacgg aggttatatt ttcactgcgg acgccgtccc ggaaatggat
     3121 accgcagtca acccgcttgc gtatgcttac ctggtaaaaa tcggcgccgg cggcgccacg
     3181 cagtggatca aaagctttcc caaatccgat tttttcatca agcctttcga tgccaactgc
     3241 gtctgcgccg cgagcgacgg cggttacctg atggccacct gtttttattc ctcccagtac
     3301 ccggcttcat cgaataaccc tgcccgcatc ctgcttgtaa aaaccgatgc cgccggcaac
     3361 cagctctggt cgtacattta cccgggactg gggctgagcg cgtgctattg tgtaaatgaa
     3421 acgaccgacc acggattcat cgtttgcgga gagacggcgg attcggtc
//
LOCUS       DASTSF010000370         4883 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1147123,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000370 DASTSF010000000
VERSION     DASTSF010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4883)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4883)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4883
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1147123"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..563)
                     /locus_tag="VFU15_10570"
     CDS             complement(<1..563)
                     /locus_tag="VFU15_10570"
                     /inference="COORDINATES: protein motif:HMM:NF013933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HEU4718271.1"
                     /translation="MALAFFYGMLTGAVMSVMLGTVFFALVQNSIDHGVRAGIFIASG
                     VILSDCILITASWFNAEIFPEGGKTEMIVRICGATFLLVYGIMNIVRRKTLDFPETER
                     KKVPMYMLMGFALNILNPGNYIGWLAVTTNIKTVVGYTSGTAAFYYAGALCAIFGMEM
                     LISFGASFLKKFITRRFLIWVDRTVGA"
     gene            644..1522
                     /locus_tag="VFU15_10575"
     CDS             644..1522
                     /locus_tag="VFU15_10575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HEU4718272.1"
                     /translation="MQAFSESLLFLITTPMYVIFIGLEIIVSNLHRNERYSKLGFFEN
                     MYLMFINIFVDLGMRVIALFVLSNVFAHHVTQITNPWIYWPVVIVAVDFAFWVIHYVD
                     HNCRLFWAVHVTHHSSQEFNLSVGLRSSLFEPVYRFIYFIPLAFLGFKAQDIFFGYSI
                     TQLYGVFIHTQYVPKLGFFEKFLSTPSHHRVHHGANVKYLDRNMGMFLIVWDKLFGTF
                     QAEEEKVVYGLTKNIESHHPANVIFHEFKSIYHDVRKAPGFRNKLMYIFGPPGWSHDG
                     SKKTSKQLREEAGLKR"
     gene            1534..2454
                     /locus_tag="VFU15_10580"
     CDS             1534..2454
                     /locus_tag="VFU15_10580"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEU4718273.1"
                     /translation="MKKRIAVLGIGGVGGFYGGKLAQHYSGDPQAEIIFIAHGTHAEA
                     ISAHGLKISEGEKEIVVHPDAVTDELSSAGIFDVLLVCVKTYSLEGAIASVRKNVNEK
                     TAVIPLMNGIEPYELLLKEFPQAKIFRGCCYFNAYIEAPGAIRFRGGFNQVLIGFEDD
                     VLLSSVAGIFQRAGINTFSRKDISKEVWEKFIFASAIASVGSLENDSFGQVLEKPEKK
                     RLLTEMLEEVISIARAKNVFFGPGFIPGLLEKMSGYPYEARTSMQLDFSLGRKTELET
                     FTGYVVRTGRKAGVPVPSYESVYAALKQRA"
     gene            2508..3728
                     /locus_tag="VFU15_10585"
     CDS             2508..3728
                     /locus_tag="VFU15_10585"
                     /inference="COORDINATES: protein motif:HMM:NF015316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HEU4718274.1"
                     /translation="MRLSAVLLFFLAFCRFSFAGGFQVNAQSPRQMGMGHTGTGLLTD
                     ASCLFFNPGGAAFLDHRFMVNAGTNLLFHHTEYLEPAPGIYTAYMQPHVGTPFNAYAL
                     WHGTTLSAGVGVYTPFGSLAEWPDDWKGQFLIREINLKTIFIQPTVTWKVNDYIGLGA
                     GFVYATGGFEMRQGIPVQDANGNYGEATLNGSAGGYGFNAGLFIHISDNWSAGLSYRS
                     SVNVKVENGDAKFTVPSSLADYFPNTTFSSAIKLPSVLNAGIGYTNDKWKFALDLNYI
                     GWHTYDTLRIDFAEHTDKLSDIRSPRMYHDTYILRGGAQYSLNDKIQLRAGIYYDHTP
                     VKAGYLTPETPDSDRIGVTAGASWTFLQNLSADVSFLYIEAMKRTDTNIETGFSGTYK
                     TRVLSPGIGLNYQF"
     gene            3754..>4883
                     /locus_tag="VFU15_10590"
     CDS             3754..>4883
                     /locus_tag="VFU15_10590"
                     /inference="COORDINATES: protein motif:HMM:NF042769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU4718275.1"
                     /translation="MKKIFTILLASSAIFSSGLIAQTTPCDGLRYRDFIFQDSTVSNI
                     VYGSNVNYTNANTTLKLDLHFPQGDTGSMRPLIIIMHGGNFLGGSKTGTDVIQFCHDF
                     AKMGYVVASIDYRVGMTNFPLGSPDSVDAAGSVIRAVHDGKAAVRFFKKDVVTNGNSY
                     RIDTSNIYFMGISAGAITALHMAYMDNMSEFPVYADTANHYGLNGGIEGLSGNPGYSS
                     SFRAVIELCGAIGDTSWINPGDMPAMMFHGNQDGTVPYYTDTISLLGQYPLLVVDGSH
                     SVDIRLNNLGIEHCFETWEGQDHIPEVGASSTALAYYDSALVITRNFLVHYICGDPLQ
                     CGYTTNVAVNNLAPLPSLIGVFPNPATDAFTIQSDRLAGTGY"
BASE COUNT         1137 a         1332 c         1227 g         1187 t
ORIGIN      
        1 aacgcgccta cggtacggtc cacccagatg aggaacctgc gcgtgatgaa ttttttgagg
       61 aaggacgcgc cgaaagagat gagcatttcc atcccgaaaa tggcgcagag cgcaccggcg
      121 taataaaatg ccgccgttcc cgaggtatag ccgaccacgg ttttgatgtt cgtcgtcact
      181 gccagccacc cgatgtaatt gcccggattg agaatgttga gcgcaaaacc catcagcata
      241 tacatgggaa cttttttccg ctccgtctcc gggaaatcga gcgtcttcct gcgaacgata
      301 ttcatgatgc cgtaaacgag caggaacgtc gccccgcaga tgcgcacgat catttcggtc
      361 tttccgccct caggaaaaat ttccgcgttg aaccagcttg cagtgatcag gatgcagtct
      421 gacaaaataa cacccgacgc gataaagatc cctgcgcgca caccgtggtc gatgctgttc
      481 tgcaccaggg cgaaaaaaac ggtgccgagc atcacgctca tcactgcgcc ggtcagcatt
      541 ccataaaaaa aagcgagcgc caagcgaaaa ttttccggca aagctaacgg gaattccggt
      601 aactgtcatt ttccgttcct catccttgct taattttgca tccatgcagg ctttcagcga
      661 atcgctgctt tttctgatca ccacgccgat gtacgtcatc ttcatcgggc tggagatcat
      721 cgtttccaac cttcaccgca acgaacggta cagcaagctc ggcttcttcg agaacatgta
      781 cctgatgttc atcaacatct tcgtcgacct cggcatgcgc gtcatcgcgc tctttgtcct
      841 ttcgaatgtt ttcgcgcatc acgtcacgca gataaccaac ccgtggatct actggccggt
      901 ggtgatcgtc gccgtggatt ttgcgttctg ggtgattcat tatgtcgacc acaactgccg
      961 gcttttctgg gcggtgcatg tcacgcatca ttcctcgcag gaattcaacc tcagcgtagg
     1021 actgcgttct tcgctgttcg aacctgttta ccgttttatt tatttcattc cgctcgcctt
     1081 cctcggcttt aaagcgcagg atattttctt cggctactcc atcacccagc tttacggcgt
     1141 tttcattcac acgcaatatg ttcccaaact tggtttcttt gaaaaattcc tgtccacgcc
     1201 ttcgcaccac cgcgtgcatc acggggcgaa cgtgaaatac ctcgaccgca acatgggcat
     1261 gttcctgatc gtatgggaca agcttttcgg gacgttccag gcggaagagg aaaaagtggt
     1321 ttacggtctc acgaaaaaca tcgaatcgca tcacccggcc aatgttattt tccacgagtt
     1381 caaaagcatt taccatgatg tccgcaaagc gccgggattc cgcaacaagc tgatgtatat
     1441 tttcggtccg cccggctgga gccacgacgg cagcaagaag acctcgaaac aattgcggga
     1501 agaggcgggg ctgaaacgct gatttttttc gcgatgaaaa aacgtatcgc cgttctcggt
     1561 attggcggcg tcggcgggtt ctacggggga aagctggcgc agcattattc cggcgatccg
     1621 caggctgaga tcattttcat tgcgcacggc acacatgcag aggcgatatc tgcacatgga
     1681 ttgaaaattt ccgaaggaga aaaagaaata gtcgttcacc ccgatgcagt gaccgatgaa
     1741 ctttcgtccg caggaatttt cgatgtgctg ctggtttgcg tgaaaacgta cagtctcgaa
     1801 ggagcgatcg cttctgtacg gaaaaacgta aatgaaaaaa ccgcggtcat ccccttgatg
     1861 aacggcatcg agccgtatga attgttgctg aaagaatttc cgcaggcaaa aattttccgg
     1921 ggctgctgct atttcaatgc gtacatcgaa gcgccgggag cgatccgttt ccgcggcgga
     1981 ttcaaccagg tgctcatcgg tttcgaagac gatgtcttgt tgtcttctgt cgctggcatt
     2041 tttcagcgcg ccggaataaa tacattttcc cgaaaagata tttccaaaga agtgtgggag
     2101 aaattcatct tcgcttccgc gatcgccagc gtgggctcac tggaaaatga ttcgttcggc
     2161 caggtcctcg aaaagccgga aaagaagcgc ctgctcaccg aaatgctgga agaggttatt
     2221 tcgatcgcgc gtgcgaaaaa tgtttttttc gggcctggtt tcattcccgg cctgcttgag
     2281 aaaatgtccg gctacccgta cgaagcgcga acgtccatgc agctcgattt ttcacttgga
     2341 cgcaaaacag agctggaaac atttacggga tacgtggtgc ggaccggccg gaaagcggga
     2401 gttcccgtgc cttcctacga atcagtttac gctgcattga agcagcgcgc ctgatttctg
     2461 cattgcaccg gggtgaacac attctgaatg ttttgttaaa tttgagaatg aggctttccg
     2521 ccgtactcct tttctttctc gctttttgcc gtttttcatt cgccggggga ttccaggtga
     2581 atgcgcaaag cccgaggcaa atgggaatgg gacacaccgg aaccggtttg ctcaccgatg
     2641 catcctgtct tttcttcaat cccggcggcg ccgcctttct cgaccaccgc ttcatggtga
     2701 atgcgggcac gaacctgctc ttccaccaca cggaatacct cgaaccggct ccgggaattt
     2761 acaccgccta tatgcagccg catgtcggca caccgttcaa tgcgtatgcg ctctggcacg
     2821 gaacaacact ttccgcaggc gtcggtgttt acacgccgtt cggcagtctt gcagaatggc
     2881 cggacgactg gaaaggacaa ttcctgatcc gggaaataaa cctgaaaacg atcttcatcc
     2941 agcccacggt aacgtggaag gtcaacgatt acatcgggct gggcgcaggt ttcgtgtacg
     3001 ccaccggcgg gtttgaaatg cggcagggaa ttcccgtgca ggatgcgaac ggcaattacg
     3061 gagaagcgac gctcaacggt tccgcgggcg gttacggttt caacgccgga cttttcattc
     3121 acatcagcga caactggagc gccggtcttt cctatcgatc gtcggtgaat gtgaaagtgg
     3181 agaacggcga tgcgaaattt accgttccct cttcgctggc ggattatttc ccgaacacca
     3241 cgttctcttc cgcgataaag ctgccttctg ttctcaatgc cggcatcggt tatacaaacg
     3301 acaaatggaa gttcgcgctc gatctgaatt acatcggctg gcacacatat gatacgctgc
     3361 gcatcgattt cgcggaacac accgacaaac tgagcgacat ccgttccccg cgcatgtacc
     3421 atgacacgta catccttcgc gggggcgcgc aatattcgct taatgataag atccagctgc
     3481 gtgccgggat ttattacgac cacacgccgg tgaaagcagg ttatctcacg ccggaaactc
     3541 ccgacagcga ccgcatcggt gtaacagccg gagccagctg gacgttcctt caaaatcttt
     3601 cggccgacgt ttcattcctt tacatcgaag cgatgaaacg taccgacacc aatatcgaaa
     3661 ccggtttttc cggaacgtac aaaacacgcg ttctgtcgcc gggtatcggg ctcaattacc
     3721 aattctgaaa tgaattcatc aattccgttc accatgaaaa aaatctttac catccttctc
     3781 gcctcttcag ccattttctc ttcggggctt attgcgcaaa caacgccatg cgacggcctg
     3841 cgttaccgtg atttcatatt ccaggacagc accgtgtcga acatcgtgta tggctcgaat
     3901 gtgaattata ccaatgcaaa taccacattg aaactcgacc tgcattttcc ccagggcgat
     3961 accggcagta tgcggccgct catcattatc atgcacggcg ggaattttct cggcggatcg
     4021 aaaaccggca cggacgtgat ccagttctgt cacgactttg caaaaatggg gtacgtggtc
     4081 gcgtccatcg attatcgcgt gggcatgacg aatttcccgc tgggctcgcc tgattctgtg
     4141 gatgcggccg gttccgtgat ccgcgctgtg catgacggaa aagcggctgt gcggttcttc
     4201 aagaaagatg tggtcaccaa tggaaattcc tatcgcatcg atacgtcgaa tatttatttt
     4261 atgggtattt cggccggcgc catcaccgcg cttcacatgg cttacatgga caacatgagc
     4321 gaattcccgg tgtatgcgga tacagccaat cattacggac tgaacggagg aatcgaagga
     4381 cttagcggaa acccgggata ttcgtcttcg ttcagggccg tgatcgaatt gtgcggcgcg
     4441 atcggtgata cttcctggat caatcccgga gatatgccgg cgatgatgtt ccatggcaac
     4501 caggacggaa cagttcctta ttacacggac acgatctccc tcctcgggca gtatccgctg
     4561 cttgttgtgg atggctcaca ttccgtcgac atccgcctga acaacctggg aatcgaacac
     4621 tgctttgaaa cctgggaagg ccaggatcat atccctgaag tcggagcaag cagcactgcg
     4681 ctcgcctatt atgattctgc actcgtgatc acacggaatt tcctcgtgca ttatatctgc
     4741 ggcgatccgc ttcagtgcgg ttacaccacg aatgtggccg tgaacaatct cgccccgctt
     4801 ccttcgctca tcggtgtttt cccgaatccc gcgaccgacg cattcaccat ccagtcagat
     4861 cgtctcgcag gaaccggtta cat
//
LOCUS       DASTSF010000371         5660 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_709066,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000371 DASTSF010000000
VERSION     DASTSF010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5660
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_709066"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1250
                     /locus_tag="VFU15_10595"
     CDS             <1..1250
                     /locus_tag="VFU15_10595"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011586981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEU4718276.1"
                     /translation="IASKVSDALKTALLKQSNLFESLQFRYFGPIDGHDVDRLVKVMQ
                     DLKDIPGPKLLHVLTTKGKGFKFSEEGNPTLWHSPGLFDKHTGKLVKPVSNEPQPPKY
                     QDVFGNTIVELAEKNPKIMGITPAMPTGCSLNIMMKAMPDRAFDVGIAEQHAVTFSAG
                     LATQGMIPFCNIYSSFMQRAYDQVIHDVALQNLHVVFCLDRGGLAGSDGPTHHGAFDL
                     AYFRCIPNMVVSAPMNEPELRNLMYTAQLEKNNFPFSIRYPRGAGVTPEWKTPFEEIK
                     VGTGRKIRAGKDIAILTIGHPGNFAVDACARLEKAGVDAAHYDMRFVKPLDEKMLHEI
                     FTKFSKVITVEDGSVQGGFGSAVLEFMSDHQYSAEVKRLGIPDRFIEHGEQAELWNEC
                     HFDADAITETAKKMVLKTGKVLR"
     gene            complement(1608..2561)
                     /gene="metF"
                     /locus_tag="VFU15_10600"
     CDS             complement(1608..2561)
                     /gene="metF"
                     /locus_tag="VFU15_10600"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008623612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HEU4718277.1"
                     /translation="MKVIDHLHAAKSTLFSIEILPPLKGTSIQSIYNGIDPLMEFKPS
                     FVDVTYHREEYIYKKRDGGYLEKVSIRKRPGTVGICAAIMNRYHVDAVPHIICGGFTK
                     EETESALIDLHFLGIDNVLLLRGDAIKTESAFVPEPNGHNYAIDLVKQVVQMNNGKYM
                     HEDVVDMAPTNFCIGVAGYPEKHFEAPNLIADMKHLKAKVDAGAEYIVTQMFFDNSKY
                     FEFVKKCREQGISVPILPGLKILTGKKQILSLSKIFHIDIPQALYEEIEKCKDDKEVR
                     EVGIRWCIQQAKELVKAKAPCLHFYTMGSSDATRRVANEVF"
     gene            complement(2589..5393)
                     /gene="infB"
                     /locus_tag="VFU15_10605"
     CDS             complement(2589..5393)
                     /gene="infB"
                     /locus_tag="VFU15_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017259736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEU4718278.1"
                     /translation="MSDADKTIRLSKVKGELNVSLDRIFEFLDEKGFSLERNPNQKIS
                     EDIYRLLMQEFAQDREEKEESKLVGLSTRTKKESIILDEETSRSEKREREREQDEVLI
                     KDMGSGGKTVDTAKEKKAEPEPEEVIRAKAEKTVGVKVVSKIDLGPAKEKGKKKKEEA
                     EEKPAKAKEETKEKKPTRKKKEEEPAEEEPVAETKKPEEEIPAVVPPPQEEPVPEKQE
                     EVLFRRTVEKLEGPKIMGKIELPVEPDKKKPVASSSAKFEAEKKKKRKRIRKEGGPVN
                     LQEESKQGGRGRNQRGGNQREAKAELTEEEVQQQIKETLARLSGQGKSKASKYRREKR
                     DQVSARIEKEQAEIEAQKGVLKVTEFVTANQLASMMSIPVTQIISTCMTLGLFVSINQ
                     RLDAEIITMLAEEYGYKVEFVSVEVQESIQEEEDTPEQLADRPPIVTVMGHVDHGKTS
                     LLDYIRKTNVIAGESGGITQHIGAYSVQLENGKHITFLDTPGHEAFTAMRARGAQVTD
                     VAIIVVAADDSVMPQTIEAINHAQAAGVPIVFAINKIDKPGANPDKIREALANMNILV
                     EEWGGKYQCQEISAKKGLNIETLLEKVLLEAELLQLKANPTRRATGTIIESALDKGRG
                     YTATILVENGTMRIGDVVLAGCYSGRVKAMHNERGLDVEEAGPATPVQILGLSGAPQA
                     GDKFNVMADEREAREIATRRLQLQREQQIRTTKHITLDEIGRRLAIGDFKELNIIVKG
                     DVDGSIEALADSLLKLSTEKIQVRIVHKGVGAISESDVLLASASNAIIIGFQVRPSSN
                     AKKLAETEQIDIRLYSVIYKAIDEVKAAMEGMLAPEYEERIVCNVEVREVFKISKVGT
                     IAGCYVLDGKISRNTKVRLIRNGIVLHDGALGSLKRFKDDVKEVASGYECGLNIDKFD
                     DVQVGDLIEGYEMVEVKGKL"
     gene            complement(5457..>5660)
                     /locus_tag="VFU15_10610"
     CDS             complement(5457..>5660)
                     /locus_tag="VFU15_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017843143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HEU4718279.1"
                     /translation="TDEDVEDVKLDEFNDEIEQWIIDEFKSIGCDTAKAVLELSDEEL
                     VKRTDLEEETVKEVKRILRSEFE"
BASE COUNT         1112 a         1562 c         1458 g         1528 t
ORIGIN      
        1 agatcgcttc gaaagtgagc gatgcgctga aaacagcgct gctcaagcaa agcaatcttt
       61 tcgaatcgct gcagttccgt tatttcggcc cgatcgacgg gcatgatgtc gaccgcctcg
      121 tgaaagtgat gcaggacctg aaagatattc cgggtcccaa gctgctgcac gtgctgacga
      181 caaaaggaaa aggattcaaa ttttccgaag aaggaaatcc aacgctttgg cattcgcccg
      241 ggctgttcga caagcacacg ggcaagctcg tgaagccggt ttcgaatgaa ccgcagccgc
      301 cgaaatacca ggatgttttc ggcaacacga tcgtcgagct cgcggaaaaa aatccgaaga
      361 tcatgggcat cacgcccgca atgccgaccg gctgctcgct caacatcatg atgaaagcca
      421 tgccggaccg cgcgttcgat gtgggcattg ccgaacagca tgcagtgaca ttttccgcgg
      481 gactcgccac gcagggcatg atcccgttct gcaatattta ttcgtcgttc atgcagcgcg
      541 cttacgacca ggttatccac gacgtggcgc tgcagaacct ccacgtggtt ttctgcctcg
      601 accgcggagg acttgccggt tccgacggcc ccacgcatca cggcgcattc gatcttgcat
      661 atttccgttg catcccgaac atggtggtca gcgcgccgat gaacgagccc gagctgcgca
      721 acctcatgta cacggcgcag ctggaaaaaa ataatttccc tttttccatc cgctacccgc
      781 gcggcgcagg cgttacaccg gaatggaaaa caccgttcga agagatcaaa gtcggaacgg
      841 gcagaaagat ccgcgccggg aaagacattg cgatactcac gatcggtcac ccgggaaatt
      901 ttgcagtgga tgcatgcgca cggctggaga aagccggggt ggatgcggcg cattacgata
      961 tgcgcttcgt gaagccgctc gacgaaaaaa tgctgcacga aatttttacg aagttcagca
     1021 aagtgatcac ggtggaagac ggcagcgtgc agggcgggtt cggaagcgcg gtgctcgagt
     1081 tcatgagcga tcatcagtat tccgccgagg taaaacgtct cggcattccc gaccgtttca
     1141 tcgagcacgg tgaacaggcc gagctctgga acgaatgtca tttcgatgcg gatgcgatta
     1201 ccgaaaccgc gaagaaaatg gtgctgaaga cggggaaagt gctgcgttga aaaattccgg
     1261 gataccttct tgaatcggcc ttgtgtgatc acaatcacgc gtcattgtgc gtttggtcaa
     1321 agacttttct tcttccttac cgcatctttg taacatcttc tttgaaatac acgggctgag
     1381 aaaagaaagc agacataacc gtttaaaaaa cgactaaact gctttcaaac aggtcggcgg
     1441 gttcactccc ggctgagact taaccgtgca ttttcaaaag aggaaagttt ttcgtggaat
     1501 agctgctgca aaaggcaggt aaaaccaccc caaacgattc gaaggcgctc tatacggacg
     1561 ccttcttttt tttatgcact gagccgggaa ttattatgaa atacgtctta aaaaacttcg
     1621 ttcgccactc ttcgcgtagc gtccgagctt cccatcgtgt agaagtgcag gcagggcgct
     1681 ttcgctttca ccagctcctt cgcctgctgt atgcaccagc gtatccccac ttcacgcact
     1741 tctttgtcgt ctttgcattt ttcaatctcc tcgtacagcg cctgtggaat atcgatgtgg
     1801 aaaatttttg acagcgaaag gatctgtttt ttcccggtaa ggattttcag tccgggcagg
     1861 atgggaacgc tgatgccctg ttcgcggcat tttttcacga actcgaaata tttcgaattg
     1921 tcgaagaaca tctgcgtgac aatgtattcg gcacctgcat cgactttcgc cttgaggtgc
     1981 ttcatgtcgg cgatgaggtt cggcgcttca aaatgttttt caggataacc ggccacgccg
     2041 atgcagaaat tcgtgggcgc catgtcgacg acatcttcgt gcatgtattt cccgttattc
     2101 atctgcacga cctgcttcac caggtcaatg gcataattat ggccgttggg ttccggaacg
     2161 aatgccgatt cggttttgat ggcgtcgccg cgcagcagca gcacattgtc aatgccgagg
     2221 aagtgcaggt cgatgagcgc gctttccgtt tcttctttcg tgaaacctcc gcagatgatg
     2281 tgcggaacag catccacgtg ataccggttc atgatcgccg cacagatgcc gaccgttccg
     2341 ggacgcttgc ggatggaaac tttttcaaga taacctccgt cgcgtttttt gtaaatgtat
     2401 tcttcgcggt gataggtgac gtcgacgaac gaaggtttga attccatcag cgggtcgatg
     2461 ccgttgtaga tcgactggat gcttgttccc ttgagcgggg gaaggatttc tatcgaaaac
     2521 aatgtcgact tcgccgcgtg aagatgatcg atgactttca taaaccgtga tctgttcgct
     2581 gcgcagtttt agagtttacc tttcacttcc accatctcgt atccttcgat gagatcgccc
     2641 acctgcacat cgtcgaactt gtcgatgttg agtccgcatt cgtagcccga cgccacttct
     2701 ttcacgtcgt ccttgaaacg cttgagcgaa cccagcgcac cgtcatgcag cacgatgccg
     2761 ttgcggatca ggcgcacttt ggtgttgcgt gaaatttttc catcgagcac atagcacccg
     2821 gcgatcgttc ccactttcga gatcttgaaa acctcgcgca cttccacgtt gcagacgatc
     2881 cgttcttcgt attccggcgc aagcattcct tccatcgcgg ctttgacttc gtcgatcgcc
     2941 ttgtagatga cagaatacag gcggatgtcg atctgttccg tttccgcgag ctttttcgca
     3001 ttggaagaag gacgcacctg gaagccgatg atgatcgcgt tcgaagctga ggcaagaaga
     3061 acatccgatt cggagatcgc accgacgccc ttgtgcacga tacgcacctg tatcttctct
     3121 gttgaaagtt tcagcagtga atccgcaagc gcttcgatgg aaccgtccac gtcgcctttg
     3181 acaatgatgt tgagctcttt gaaatcgccg atcgcaaggc ggcggccgat ctcgtcgagc
     3241 gtaatgtgct ttgtcgtgcg gatctgctgc tcgcgctgga gctgcaggcg gcgtgtggcg
     3301 atctctctcg cttcacgctc atccgccatc acgttgaact tgtcgccggc ctgcggcgca
     3361 ccgctgagac cgaggatctg cacaggtgta gccggacccg cttcttccac atcgagtccg
     3421 cgctcgttgt gcatcgcttt gacacggccg ctgtagcagc cggcaagaac gacgtcgccg
     3481 atgcgcatgg tcccgttctc gacgaggatc gttgcggtgt atcctcttcc tttgtcgagc
     3541 gccgattcga tgatcgttcc ggtcgcgcgg cgcgtaggat tcgctttgag ctggagcaat
     3601 tccgcttcga gcagcacttt ttccagcagc gtttcgatgt tgagtccttt cttcgccgaa
     3661 atttcctggc actggtattt accgccccat tcttcgacga ggatattcat gttcgccagc
     3721 gcttcgcgga tcttgtccgg gtttgcgccg ggcttgtcga tcttgttgat ggcaaatacg
     3781 atcggcacac ctgcggcctg cgcatggttg atcgcttcta tcgtctgcgg catgacgctg
     3841 tcgtcagcgg caacaacgat gatcgccacg tccgtcacct gcgcaccgcg ggcgcgcatg
     3901 gcggtaaacg cttcgtgacc cggcgtatcg aggaaggtga tgtgctttcc gttctcgagc
     3961 tgaacgctgt aagcaccgat gtgctgcgta atgccgcctg attcgcctgc gataacgttc
     4021 gttttccgga tataatcgag cagtgatgtt ttcccgtgat cgacgtgtcc catcaccgtc
     4081 acgatcggcg ggcgatcggc gagctgttcc ggggtatctt cttcttcctg gatggattcc
     4141 tgcacttcga cgctgacaaa ctccactttg tatccgtact cttccgccag catcgtgatg
     4201 atctctgcat ccaggcgctg gttgatcgat acaaaaagtc cgagcgtcat gcaggtagag
     4261 atgatctgcg tgacaggaat gctcatcatc gacgccaact ggttggcggt aacgaattcg
     4321 gttactttca ggacgccttt ctgcgcttcg atctctgcct gctctttctc gatgcgggcg
     4381 ctgacctggt cgcgtttttc acggcgatac ttcgacgctt tcgattttcc ctggccgctg
     4441 agacgcgcaa gcgtctcctt gatctgctgc tgcacttcct cttcggtaag ctcagctttg
     4501 gcttcccgct ggttgccgcc gcgctggttg cgtccgcgtc cgccctgttt ggattcttcc
     4561 tgcaggttga cagggccgcc ttctttgcgg atgcgtttac gtttcttttt cttttccgct
     4621 tcgaattttg cagacgagga agcgaccggt tttttcttgt cgggctcaac aggcagctcg
     4681 atcttgccca taatcttggg cccttccagt ttttccacgg tgcggcggaa gagcacttct
     4741 tcctgctttt ccggaacagg ttcttcctgt ggcggaggaa ccaccgcagg gatctcttct
     4801 tccggtttct tcgtttccgc taccggctct tcttcggccg gctcttcttc ttttttctta
     4861 cgggtgggct ttttctcttt tgtttcttct tttgctttcg ccggtttttc ctctgcttcc
     4921 tctttcttct tttttccttt ttccttcgca ggaccgagat cgatcttcga aacgactttc
     4981 acgccaacgg ttttctccgc tttggcgcgg atcacttcct ccggttcggg ttccgctttt
     5041 ttctccttcg ccgtatcgac agtctttccg ccggatccca tgtctttgat gagaacctcg
     5101 tcctgttcgc gttcccgctc acgtttttcc gagcgcgacg tttcctcgtc gaggataata
     5161 ctctcctttt tggttctcgt actgagacca accagcttgg actcttcctt ttcctcccgg
     5221 tcctgggcga attcctgcat gagaagacgg tagatgtctt ccgaaatttt ctggttcgga
     5281 ttccgctcca ggctgaatcc cttttcatcg aggaattcaa atatccggtc gagggaaacg
     5341 ttgagctccc ctttcacctt gctgagtcgt atcgttttat cggcgtctga catatttgtg
     5401 cccgctgcgg gcttaggtat tacttgatta caggaaacgg ggtatccgct tcacgtttat
     5461 tcaaattcgg aacgaagaat gcgtttcact tctttcacgg tttcttcttc gaggtccgta
     5521 cgtttcacca gctcttcatc gctgagctcg agaacggctt tcgctgtatc gcagccgatg
     5581 gatttgaact catcgatgat ccactgttcg atctcgtcgt tgaactcgtc gagcttcacg
     5641 tcttccacat cttcatctgt
//
LOCUS       DASTSF010000372         6031 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1593959,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000372 DASTSF010000000
VERSION     DASTSF010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 6031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6031
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1593959"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..247)
                     /locus_tag="VFU15_10615"
     CDS             complement(<1..247)
                     /locus_tag="VFU15_10615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014937895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEU4718280.1"
                     /translation="MPATDNSRKISYNRTKIVATLGPASSSPEMLEEMVRAGTDVFRI
                     NFSHGDYAAVESLLKNIRDINRKLSTHVSILGDLQGPK"
     gene            complement(250..669)
                     /locus_tag="VFU15_10620"
     CDS             complement(250..669)
                     /locus_tag="VFU15_10620"
                     /inference="COORDINATES: protein motif:HMM:NF033205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPExxxVDY family protein"
                     /protein_id="HEU4718281.1"
                     /translation="MTKFRLDVEYDYDFFLAGISCHEKPHRLCWAINKSLECSLARTE
                     SLSLSLKKNEGPASFILFSSEDKAHDAAFFLVSNKCETGTLVPEHHQADFFYIVKGPY
                     SGEDQERMLRELRTIPFVLMAYRIDPASLKSKQNLLF"
     gene            775..1197
                     /locus_tag="VFU15_10625"
     CDS             775..1197
                     /locus_tag="VFU15_10625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718282.1"
                     /translation="MKTIPLTLFFLSFVLLLGAQTPKYALLDAKDPVVGTWEWIGNDT
                     GSMAAPVPDLNWQFIQLAAGSDQSMGALSYSDEKGYECASYFLAFSNGQMITGTLSQT
                     CLEADKGKKFTMGYRYDAMNDQLVLTIRGETFYYRRKS"
     gene            complement(1284..1934)
                     /locus_tag="VFU15_10630"
     CDS             complement(1284..1934)
                     /locus_tag="VFU15_10630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016549648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEU4718283.1"
                     /translation="MKIGVLGTGMVGNTIAGKMIQLGHEVKMGSRSATNEKAAEWVKS
                     AGKNASAGTFADAAAFGEIIFNCTKGDATMEVLKLAGEKNLGAKLFIDISNPLDFSKG
                     FPPTLTICNDDSLGETIQRTYPSLKVVKTLNTMNCLLMVNPSLVPGEHNVFLSGNDAD
                     AKAAVKKLLGEIGWTEKSMIDVGDITTCRGVEQLLPIWVRLYGAFGSPNFNFHIAR"
     gene            complement(2000..2374)
                     /locus_tag="VFU15_10635"
     CDS             complement(2000..2374)
                     /locus_tag="VFU15_10635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718284.1"
                     /translation="MNAIAASLHWICLATGGWLTLNGVLHDIFVLASDHGKKYDRDLL
                     RLLMDGHILITCGIVLMLCFPGLRSGQQFFFYIAALCLVSIIGYCFMIWPFLKSIGTL
                     VICTACLIAVVFTFFTTPTSHV"
     gene            complement(2386..3060)
                     /locus_tag="VFU15_10640"
     CDS             complement(2386..3060)
                     /locus_tag="VFU15_10640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012625846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU4718285.1"
                     /translation="MKTAVVTGGNKGIGREIVRQLKQEGFHVVLAARDEKRGSTAAAE
                     EGAEFIRMDVADEKSILEFAKQFGKKFNHLDVLVNNAGILKKEDRSVLASSQELMNET
                     FAVNAYGPVRVTKALLPFLGNGSRVIMVSSGGGSMSDDIAGWAPVYCVSKTLLNAFTR
                     HLAHELAPKGIFVNAICPGWVRTDMGGKGAPRTVQQGADTVTWLATTDKVHSGGIFRD
                     RKKIDW"
     gene            3199..3687
                     /locus_tag="VFU15_10645"
     CDS             3199..3687
                     /locus_tag="VFU15_10645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718286.1"
                     /translation="MKKTYTPELIERLCSALTRDEDSFRWLMDNHERELAALCDVLVY
                     GKEDAAEWLKNNGFNVIYSFIAVLDENEQAQKYLMDHDAKEWAATADAVNGSDSAYEW
                     LKQYYPHFAHLSDVLSSLSNGRGGMGGFSGGSGGGGGIGGGGFGGYGGGGFGGGGAGG
                     KW"
     gene            3704..4120
                     /locus_tag="VFU15_10650"
     CDS             3704..4120
                     /locus_tag="VFU15_10650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017584582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU4718287.1"
                     /translation="MIIDGKEVEIRIGENRFTCAMDLTMSVIGGKWKTVVLWYLKSKP
                     KRFSELKRHVPGISEKMLSLQLREMERDGIVKRTVFPEVPPRVEYELTKEGKTLIPAL
                     NAIAAWGREKAKRDGVITEVKKRGKKTGKKIQRTGP"
     gene            complement(4102..4674)
                     /locus_tag="VFU15_10655"
     CDS             complement(4102..4674)
                     /locus_tag="VFU15_10655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718288.1"
                     /translation="MRIPRSLFFLSLVLFSVSLRAQLYVGIKFMGLSFHPGKNTNAQF
                     YDVTIGKKKKLVLNFGVALTAEYMVYRNFSVKYDQALFRDCAGKFAGMSMFNVRYTID
                     MRDLGLGSAGIGPFFFYRKTWTDFDGYDDEGFFRLGKNRKWQTKFVWYGGELEHDYPL
                     KNSWWISTNLLPGIPVVYALAPGMRYGPVR"
     gene            4753..4826
                     /locus_tag="VFU15_10660"
     tRNA            4753..4826
                     /locus_tag="VFU15_10660"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4787..4789,aa:Ala,seq:ggc)
     gene            complement(5044..5892)
                     /gene="dinD"
                     /locus_tag="VFU15_10665"
     CDS             complement(5044..5892)
                     /gene="dinD"
                     /locus_tag="VFU15_10665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008630984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HEU4718289.1"
                     /translation="MKRELIIELFQKLESIKLLQDGIEYWSARDMQEILGYSQWKNFQ
                     KVLSKAKEACINSGVESADHFADIGKMIELGKGAVREVEDVALTRYGCYLVAQNGDAS
                     KSEVAFAQTYFAVQTRKQEIIEQRLLDVARVTAREKLTKSEKKLSGILYERGVDDRGF
                     AIIRSKGDQVLFGGHSTQMMKKKLGVPEGKPLADFLPTLTIKAKDFANELTSHNVEVK
                     DLKGQDAISNEHIENNKAVRKMLHERGVKPENLPPAEDVGKLKRKLESEDKKLLKEVK
                     KKSKGK"
BASE COUNT         1452 a         1500 c         1613 g         1466 t
ORIGIN      
        1 gcttcggacc ctgcaggtcg ccgagaatgg aaacgtgcgt ggaaagcttg cggttgatgt
       61 cgcgtatgtt cttcagcagc gattctaccg cggcgtaatc cccgtgcgaa aaattgatgc
      121 ggaaaacgtc cgtgccggcg cgcaccattt cctcgagcat ttcgggagag gaggaagcgg
      181 gtccgagggt ggcgacgatc tttgtgcggt tgtaggaaat tttcctggaa ttgtcggtag
      241 ctggcatgat cagaataaga ggttttgttt ggatttcaga gaagcgggat cgatgcggta
      301 cgccatgagc acgaacggga tcgtgcgcag ctccctgagc atgcgttcct ggtcttcacc
      361 ggaatacggt cctttgacga tgtaaaaaaa atcggcctgg tggtgttccg ggacaagcgt
      421 gcctgtttca catttgttcg agacgagaaa aaaagcggcg tcgtgggcct tgtcttccga
      481 ggaaaaaagg atgaacgaag cgggaccttc gttctttttc agggaaagcg aaagcgattc
      541 ggtccgcgcc agcgagcatt ccagcgattt gttgatcgcc cagcaaagcc ggtggggttt
      601 ttcatgacag gaaatccctg ccaggaaaaa atcgtaatca tactcgacat cgagacggaa
      661 ttttgtcacc gcgctgcatt tcaatctgca cgaagatacg aatcgcggtt ttcaaaatgg
      721 ttatgaattg gtcatccttc cgccaattcg taaattcgtt ttctccggga aaaaatgaaa
      781 acgataccgc tgaccctctt ctttctttcg ttcgtcctgc tgctgggtgc gcagacgccg
      841 aaatacgcat tgctcgatgc caaagatccc gtcgtcggca catgggaatg gatcgggaac
      901 gacaccggct ctatggcggc cccggttccc gacctgaact ggcagttcat ccaattggcg
      961 gccggcagcg atcagtcgat gggtgcgctt tcctacagtg atgaaaaagg atacgaatgc
     1021 gcttcctact tcctcgcttt ctccaacggg cagatgatca ccggaacgct ttcgcagacg
     1081 tgcctggaag ccgacaaggg aaagaagttc accatgggat accgctacga tgcgatgaac
     1141 gaccagctcg tgctaacgat acgcggcgag acgttttatt acaggcgaaa aagctgatac
     1201 ggtattccgc aggaaccgtg ctgaaaacac atgaccggag gtaatcgaaa acagaaaacc
     1261 tccggctgcg tcgggttatg ctgtcagcgc gcgatatgaa aattgaaatt gggtgaaccg
     1321 aatgcgccgt aaagacgcac ccagatcggg agcagctgtt cgacgccacg gcaggtggtg
     1381 atatcgccga catcgatcat gcttttttcc gtccagccga tctcgccgag gagttttttc
     1441 acggccgcct tcgcatccgc atcgttgccg ctgaggaaaa cgttgtgctc tcccggaacg
     1501 agggaaggat tgaccatgag gagacagttc atcgtgttga gcgtcttcac cactttgagc
     1561 gaaggataag tgcgctggat cgtttcgccg agcgaatcat cgttgcagat ggtgagcgtg
     1621 ggcgggaaac ctttcgaaaa atcgagcggg ttggaaatgt cgatgaagag ctttgcaccg
     1681 aggttttttt cacccgccag cttcagcact tccatcgttg catcgccttt cgtgcagttg
     1741 aagatgattt cgccgaatgc ggctgcatcc gcgaacgtgc ctgccgaagc atttttcccg
     1801 gcgcttttca cccattccgc agctttttca ttcgtggcgc tgcgcgaacc catttttact
     1861 tcatgaccca gctggatcat ttttccggcg atcgtatttc cgaccattcc ggtaccgagt
     1921 actcctattt tcatttttat gcggttttag acattagcta tgagacgtga gattgaagtt
     1981 ggaggaagtg acaatgaaac tatacatgag aagtgggtgt ggtgaaaaat gtgaatacaa
     2041 cggcgatgag acaggccgtg cagatgacga gcgtgccgat cgatttcagg aaaggccaga
     2101 tcatgaagca atagccgatg atgctgacga gacacaaagc ggcgatgtaa aagaagaact
     2161 gctggcccga acgtagtccg ggaaaacaaa gcatgagcac gatcccgcac gtgatgagaa
     2221 tgtgtccgtc cataagcagg cgcagcaggt cgcggtcgta ttttttcccg tgatcggacg
     2281 cgagcacgaa aatgtcgtgg agcacgccgt tcagcgtaag ccatccgccg gtggcgaggc
     2341 aaatccagtg aagcgaagcg gctattgcgt tcatcggttc ccgttttacc agtcgatctt
     2401 tttccggtcg cgaaagatcc ctccggaatg aactttgtcc gtcgtggcca gccacgttac
     2461 ggtgtcggct ccctgctgca cagtacgcgg cgcgcctttt ccgcccatgt cggtgcgcac
     2521 ccatcccggg caaatggcat ttacgaaaat tcctttcggc gcgagctcgt gcgcgagatg
     2581 acgtgtgaac gcattgagca gtgttttcga aacgcagtaa accggtgccc agccggcgat
     2641 gtcgtcgctc atgcttccgc cgccgctgga aaccatgatc acgcgcgaac cgttcccgag
     2701 aaaaggcagc agcgcttttg tgacacggac cggtccgtat gcattgaccg cgaacgtttc
     2761 gttcatcagc tcctgtgatg aggccaggac ggaacggtct tcttttttga gaatgcccgc
     2821 gttgttgacg agcacgtcga ggtgattgaa ttttttaccg aactgtttcg caaattccag
     2881 gatgcttttt tcatcggcaa cgtccatgcg gataaattcc gcaccttctt ccgcagcagc
     2941 cgtgcttccc cgtttttcat cgcgtgccgc cagcaccacg tgaaatcctt cctgtttcag
     3001 ctggcggacg atctcgcgtc ctatgccttt attcccaccg gttacgactg ctgttttcat
     3061 gctgttggtt aaagcaaatg tacgccgggc taactttttg gcaagtactg taagaaatgt
     3121 tagtattgca taaaaatgac ggaatagcgt cgcagtgcag gaatcggagc aggataaata
     3181 aattctattg cagcataaat gaagaaaacg tacacaccgg aattgatcga gcggctttgc
     3241 tctgcactaa cgcgggatga agattctttc cgctggctga tggataacca tgagcgggag
     3301 ctggccgccc tgtgtgatgt gctggtttac ggaaaggaag atgccgcaga gtggctgaag
     3361 aataacggtt tcaacgtgat ctattccttc atcgccgttc ttgacgagaa tgagcaagct
     3421 cagaaatacc tgatggatca tgacgcgaaa gagtgggctg caacggctga cgctgtgaac
     3481 ggaagcgaca gtgcatacga atggctgaaa cagtattacc cgcatttcgc gcatttgtcg
     3541 gatgtgcttt cgtcgctcag caacggcagg ggaggcatgg gcggattcag tggcggaagc
     3601 ggcggtggcg gcggcattgg tggcggggga ttcggaggat acggtggtgg cggattcggt
     3661 ggtggcggtg ccgggggtaa atggtaacaa aaaaaacgcg ggcatgatca tagacggaaa
     3721 agaagtggaa atacgcattg gcgaaaacag gtttacctgc gccatggacc tgacgatgtc
     3781 ggtcatcggc gggaaatgga aaacggtggt gctctggtac ctgaaaagca agccgaaacg
     3841 tttcagcgag ctgaagcgtc atgtgccggg catttcggaa aaaatgcttt cgctgcagct
     3901 gcgtgagatg gaaagggacg gcatcgtgaa gcgcaccgtg ttcccggaag ttccgccgcg
     3961 cgtagagtac gagctgacga aggaaggcaa aacgcttatt cccgcgctga acgcgatcgc
     4021 ggcatgggga agggaaaaag caaagcggga cggcgtgatc accgaggtga aaaaaagagg
     4081 aaaaaaaacc gggaagaaaa ttcagcgcac aggtccgtaa cgcatgccgg gtgcgagcgc
     4141 gtacacgacg ggaatgccgg gaaggagatt ggtcgagatc caccagctgt ttttcagcgg
     4201 gtaatcatgc tcgagttccc cgccgtacca gacgaatttc gtctgccatt tccggttttt
     4261 gcccaggcgg aaaaaacctt cgtcgtcgta cccgtcgaaa tcagtccacg ttttcctgta
     4321 aaagaagaac ggtcctatcc cggcggagcc aagacccagg tcgcgcatgt cgatcgtgta
     4381 gcggacgttg aacatggaca tgccggcgaa ttttccggcg cagtcgcgga acagcgcctg
     4441 gtcgtatttc acggaaaaat tccggtacac catgtattca gccgtcagcg caacaccgaa
     4501 gttcagcacc agcttttttt tcttcccgat cgtcacgtcg tagaactgtg cattcgtgtt
     4561 tttcccggga tgaaacgaaa ggcccatgaa ctttatgccg acataaagct gcgcccggag
     4621 cgaaacagaa aaaagcacga gcgaaagaaa aaacagggag cggggaatgc gcatgaggag
     4681 gatcaaaact acggcgaaaa tggcgcgtcc tcccgaccga aattgacttt aatttctatt
     4741 tttgtcaccc ttgggggttt agctcagctg gctagagcgt ttgcatggca tgcaaaaggt
     4801 caccggttcg actccggtaa tctccacagg aaaggttcat cggaaaagat gaaccttttt
     4861 tattggaacc gacacaactg atcgcgggtt cccgggacgt ttgcatggca tgcaaaaggc
     4921 caccggttcc ctgcccgact gaagtcgttc aggcggggac tccggtaatc tccactccca
     4981 tcaaaaacgc ctcattatga ggcgttttta tttttaaaca gttgcaatat cgagccggtt
     5041 cttctattta ccctttgact ttttctttac ttccttgagg agctttttgt cttcgctttc
     5101 aagcttacgc tttagcttgc caacatcttc tgcgggagga aggttttcgg gttttactcc
     5161 tcgttcgtgg agcattttcc gaacggcctt attattctcg atatgctcat tgctgattgc
     5221 gtcctgaccc ttcagatctt ttacttctac attatggctg gttagctcat tcgcaaaatc
     5281 ttttgctttt atagtcagcg tcggaagaaa gtccgccaag ggttttcctt cgggaacgcc
     5341 gagctttttc ttcatcattt gggtagaatg ccctccgaaa aggacctggt ctccttttga
     5401 acgaatgatg gcaaaccctc tatcatccac tccccgctcg taaagaattc cggaaagctt
     5461 tttttcggat tttgtcagct tctcacgcgc agtgacacgc gccacatcca aaagtctttg
     5521 ttcaataatt tcctgtttcc gggtctggac agcgaaatac gtttgagcaa atgcaacttc
     5581 cgatttggaa gcatcaccgt tttgggcaac caggtagcac ccataacgtg taagcgcaac
     5641 atcttccact tcccgcacag cgccttttcc taattcgatc attttgccga tgtcggcaaa
     5701 atgatcagcg ctttctacgc ctgaattgat acaagcctct tttgctttgg aaagcacttt
     5761 ttgaaagttc ttccactgcg agtatcccaa tatctcctgc atatccctgg cactccaata
     5821 ttcaatccca tcttgtagca gctttatgct ttccagcttc tggaataatt caataataag
     5881 ctcccttttc ataaataaac cctttgaaca aatttaaaaa ttaaacaggc cgaattgttt
     5941 tttaaacgcc tctacatcct cccaaaaccg gttcgacaaa tcccctccca tctccacaga
     6001 tgaaatgaaa ccctcgtgaa cattgcgttt g
//
LOCUS       DASTSF010000373         2556 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_173853,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000373 DASTSF010000000
VERSION     DASTSF010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2556
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173853"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1947)
                     /locus_tag="VFU15_10670"
     CDS             complement(<1..1947)
                     /locus_tag="VFU15_10670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718290.1"
                     /translation="MQRIIPHILFSVLIAAVSILEGCTAIRVGKGQVMLKKVIIKCEN
                     PDISKDEIYGYLKQKPNRKLFGANYPHLLKRGKIGKKGLLVSGSGFPFYLCIYNLVNP
                     VREKKREDRRDARYEAKKKRYENAPIKRNGHRRKEPKKRRTIGEFLRDIGEAPVVIDS
                     SLTARSHHQLTLYLDNKGYFNSTVKDTLIYPWLQRKKQKKGILCYIIVPSQPYKIRNV
                     TWDVKDPNVDYDLLTVKDSCLIRTGNNYDVDVFEAERDRITKSLRNNGYYLFTKDFIR
                     FSIDSTLGTHQMDVTILITRPQIPTSDSTWKEVAHRRFYIDHIIVKSLISLGQLKSDT
                     AVYDTLSYNDITILRNTGSIDGIPIEEKFRYKPDVLASRISFRQNIVYRQSDYEATYR
                     QLTDLRVFRQVVINPETHDSDKINIVIELFPYARQSFTAQTEVTNTGGNLGFGGSFGY
                     QNNNLFHGAEVFNFLVKGGTEAQQTITAGSSANTPFTFNTIEFGAQATLNIPRDFFPF
                     NVVTPRNRPEDQRKAQDRRTVFTSTADYQHRIDYDRSLGNLSYGYTFRYKKYVHFGIY
                     PVEINVVKVNPRPGLAALLENRDPLLLYRFTDHLIQDSRLTYVLNTKDPRFNTIYFLR
                     FDMEASGNIMRYAFSRSKADTDATG"
     gene            2008..>2556
                     /locus_tag="VFU15_10675"
     CDS             2008..>2556
                     /locus_tag="VFU15_10675"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEU4718291.1"
                     /translation="MISRQRLSFIRSLQQKKFRQENRLFIAEGAKLVHEFLASDFAVE
                     EIYSLDPSFTADAVAVGEKEMKSMSALTTPPGVLAVIRIPDVKFDPAAVAGDWSLFLD
                     GIRDPGNMGTIIRAADWFGVKNIICSGDCAETWNPKVVQASMGSLTRVSVHSRDAHDF
                     FPLMKSALPGDVLPVYGTFMDGK"
BASE COUNT          530 a          620 c          758 g          648 t
ORIGIN      
        1 gcccgtagcg tccgtatctg ctttcgaccg ggaaaaagcg tagcgcatga tattgcccga
       61 ggcttccatg tcgaaacgga gaaagtaaat ggtgttgaag cgcggatctt ttgtattgag
      121 gacgtacgtc agccgggagt cctggatgag gtggtcggtg aaacggtaca gcaggagcgg
      181 atcgcggttt tcgagcaggg cggcaagtcc cgggcgcgga ttcactttta ccacgtttat
      241 ttcaacgggg tagatgccga agtgaacgta ttttttgtag cggaacgtat aaccgtagga
      301 caggttcccc agcgaacggt cataatcgat gcggtgctgg taatcggcgg ttgaagtgaa
      361 aacggttctg cggtcctgcg ccttgcgctg gtcttccggc cggttgcgcg gcgtcacaac
      421 gttgaacgga aagaaatccc gcggaatatt gagcgtggcc tgcgcaccga attcgatcgt
      481 gttgaaagtg aacggcgtat tcgcagatga tcccgcggta atggtctgct gtgcttccgt
      541 tccgcctttc acgaggaaat tgaagacctc ggccccgtgg aaaaggttgt tgttctggta
      601 accgaacgat ccgccgaatc caaggttgcc gcccgtgttc gtcacttccg tctgcgcggt
      661 aaagctctgc ctggcgtaag ggaacagctc gatgacgatg tttattttat ccgaatcgtg
      721 ggtttccggg ttaatgacca cctggcggaa aacacgcagg tcggtgagct ggcggtaggt
      781 cgcttcgtaa tccgactggc ggtaaacgat attctgccgg aagctgatgc gcgaagcgag
      841 cacgtcaggc ttgtagcgga atttttcctc gatcggaatt ccgtcgatcg acccggtgtt
      901 gcgcaggatc gtgatgtcat tgtaggaaag cgtatcgtaa acagcggtgt cgctcttcag
      961 ctgcccgagc gaaatcagcg atttcacgat aatgtggtcg atgtaaaaac ggcggtgggc
     1021 aacttctttc cacgtgctgt cggaagtagg tatctgcggg cgggtgatga ggatcgttac
     1081 gtccatctgg tgcgtgccca gcgtggaatc gatggaaaag cggatgaaat ctttcgtgaa
     1141 aagatagtag ccgttattcc gcaatgattt cgtgatgcga tcgcgttccg cttcgaaaac
     1201 gtcgacgtcg taattgttcc ccgtgcgtat gaggcagctg tcttttacgg tcagcaggtc
     1261 gtaatccacg ttcggatctt tcacatccca cgtcacgttg cggatcttgt atggctgcga
     1321 gggaacgatg atgtagcaga gaatgccttt cttctgcttt ttgcgctgga gccacgggta
     1381 aataagcgtg tctttcaccg tgctgttgaa atagcctttg ttgtcgaggt aaagcgtaag
     1441 ctggtgatgc gagcgggcgg tgagcgagct gtcgataacc accggcgctt cgccgatgtc
     1501 gcggagaaat tctccgatcg tgcgccgctt tttcggttct tttctccggt gcccgttccg
     1561 ttttatcggc gcattttcat agcgtttctt tttcgcctcg tagcgcgcat cgcggcggtc
     1621 ctcgcgtttt ttttcacgca cggggttgac gaggttataa atgcacaggt agaacgggaa
     1681 tcccgagccg gaaacgagca gtcctttctt gccgatcttt ccccgtttca ggagatgcgg
     1741 gtaattcgcg ccgaaaagtt tccggttcgg cttctgcttc aggtaaccgt aaatttcgtc
     1801 tttgctgatg tcaggatttt cgcatttgat gatgactttt ttcagcatca cctgcccctt
     1861 cccgacacgg atggcagtgc atccttcgag aatggaaacg gccgcgatga ggacgctgaa
     1921 taaaatatga gggattatgc gttgcacgtt tggagagcgg ccttataaaa ctaatttcgc
     1981 aaagataatg ccttgattta cctgactatg atttcccggc aacgactttc cttcatccgc
     2041 agccttcagc agaaaaaatt ccggcaggaa aaccggcttt tcattgcgga aggagcgaag
     2101 ctcgtgcatg aattcctggc ctcggatttt gcggtggaag aaatttattc gctggacccg
     2161 tcctttaccg cagatgcggt tgctgtcggt gaaaaggaaa tgaaatcgat gtcggcgctc
     2221 acgacgccgc ccggcgtgct ggcggtgatc aggatacccg acgtgaaatt cgatccggcg
     2281 gcggtcgcgg gcgactggtc gctgttcctc gacggcatcc gcgatcccgg caacatggga
     2341 acgatcatcc gcgcggccga ctggttcggc gtgaagaaca tcatttgttc cggcgattgc
     2401 gccgaaacct ggaacccgaa ggtggtgcag gcttccatgg gaagtctcac gcgcgtaagc
     2461 gtgcattccc gtgatgcgca tgattttttc ccgctgatga aaagtgcttt gccgggtgac
     2521 gtgctcccgg tttacggaac attcatggat ggaaag
//
LOCUS       DASTSF010000374         3254 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_583881,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000374 DASTSF010000000
VERSION     DASTSF010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3254
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_583881"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(95..787)
                     /locus_tag="VFU15_10680"
     CDS             complement(95..787)
                     /locus_tag="VFU15_10680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEU4718292.1"
                     /translation="MITAIAIDDEPPALKVIEEFSAESGIIELQKTFTRPSEGLKHLR
                     KFPVDLLFLDIQMPSMTGIDLVREVPKEVMVIFTTAHSEFAVEGFNLSAIDYLLKPFT
                     YERFMRAVNKAEEFRHAGMRKDPEGTQHLLIRANYSLVKVPHQEILYVEGLDDYIKIH
                     LPDRSPLVARMTMKNILEKLPSREFIRVHRSFIVPLSKIESVRNKTISIGDVKIPIGL
                     SYEENFFKNFRE"
     gene            complement(784..1812)
                     /locus_tag="VFU15_10685"
     CDS             complement(784..1812)
                     /locus_tag="VFU15_10685"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU4718293.1"
                     /translation="MDSKAGKIALHVIACAGFLSLPVIFSPDFDFSFRVLSTRSFLKD
                     LVSYTLAIGYFYLNFYVLIPRLYFTKSYAKFAVVSLLCFIVIILIPFFLFPNEWDVIN
                     TQPLAPVPGHSHSDFFFKAQPGFFRFLLVFAVSLLIKTNNRLKQAEQEKMSAELSFLR
                     AQINPHFLFNTLNSIYSLAITRSDKTAGSVVRLSEMMRYVTTEAHSEFVPLEKEIKYV
                     TNYIELQQVRLGDTVKIDFQVNGTSRTKKIIPLVLMTFVENAFKYGVNPEEDSYIVIR
                     IDDNDRDIVLDIRNRKVQTDYSKEYHSGQGIENTRQRLLMSYPGSHILEIRDKDEEYS
                     VHLQIQLS"
     gene            complement(1964..2386)
                     /locus_tag="VFU15_10690"
     CDS             complement(1964..2386)
                     /locus_tag="VFU15_10690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012796888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU4718294.1"
                     /translation="MRQKLNLITLGVSDFERAVDFYEKGLGWKRSKASVDGLALFPLG
                     GMTLALHPREELAKDATVPDEKCGFAGLSISYNAKSEKEVDEVMKQVESLGAVIVKPA
                     QKVYWGGYSGYFRDPDGHLFEVAFNPFWELDEQDNLRL"
     gene            2472..>3254
                     /locus_tag="VFU15_10695"
     CDS             2472..>3254
                     /locus_tag="VFU15_10695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718295.1"
                     /translation="MSATKLLPELSEDHPRALPWVMLLCALGLFAYVAIRSATIDITI
                     DEAYSWKFFSYGPHLYPQKYDVTTANDHWLNSWLVQLTGGVFGPRNWALRLPNVLAFA
                     VYLFFTARFVLSFRARWYTLAAFLLLNVHPYLLDFFSAERGYGLAFGAMSACIFFLFR
                     FSGSLARKDLCFFLLAGCLAVLANFAVISFFMVALGMAVLLTLLSKNADRKKLLLLTV
                     CVSVPFLAIVVPHLFHMQHSGALYYGENSLWEGTAKSVFTSLL"
BASE COUNT          702 a          908 c          832 g          812 t
ORIGIN      
        1 acacggtaat taatacgcca acttacacca aatgttatgc gggttcatga gaacaggcgc
       61 gctttgaggc caacccccgc gatcctgcgg ccaatcattc ccggaagttt ttgaagaaat
      121 tctcttcata gctgagaccg atgggaatct tcacgtcccc gatggaaatg gtcttgttac
      181 gcacgctttc gatcttcgag agcgggacaa tgaaggaacg gtgcacacgt atgaattcgc
      241 gcgaaggaag cttttccagg atatttttca tcgtcatccg cgccaccagc ggcgaacggt
      301 ccggcaggtg gatcttgatg taatcatcga gtccttccac gtacaggatc tcctggtgcg
      361 gaactttcac gagactgtaa ttcgcgcgga tgagcaggtg ctgtgttcct tcgggatcct
      421 tgcgcatgcc ggcgtgacgg aattcctccg ctttgttcac cgcccgcatg aagcgttcat
      481 acgtgaacgg tttcagcagg taatcgatcg cgctgaggtt gaatccttcc acggcaaatt
      541 cgctgtgcgc ggtggtgaag atcaccatca cttctttcgg aacttcccgg acaagatcga
      601 tgccggtcat cgagggcatc tgtatgtcga ggaaaagaag atcgaccggg aatttccgca
      661 ggtgcttcag tccttcgctg ggccgtgtga atgttttctg cagttcgatg atcccggact
      721 ctgccgagaa ttcctcgatc actttgagcg caggcggctc gtcgtcgatg gcaatggctg
      781 ttatcatgag agctgtatct gtaagtgaac actgtattct tcgtctttgt ccctgatctc
      841 gagaatatgc gaaccgggat acgacatcag caggcgctgc cgcgtatttt ctatcccctg
      901 cccgctgtgg tattccttgc tgtaatccgt ctgcactttc cggttccgga tatcgagaac
      961 aatatcgcgg tcgttgtcgt cgatccggat cacgatatac gaatcctcct ccgggttgac
     1021 gccgtacttg aacgcattct ccacgaaggt catcagcacg agcgggatta ttttcttcgt
     1081 ccgtgaagta ccgttcacct ggaaatcgat cttcaccgta tcaccgagcc ggacctgctg
     1141 cagctcgatg taattcgtca cgtacttgat ctctttctcc agcggaacga attcgctgtg
     1201 cgcttccgtg gttacataac gcatcatctc ggaaagccgc accacggatc ctgccgtctt
     1261 gtcggaacgc gtgatggcga gcgaataaat gctgttgagc gtattgaaca ggaaatgcgg
     1321 gttgatctgc gcgcgcagga aagaaagttc tgcgctcatc ttttcctgct ccgcctgctt
     1381 gagccggttg ttggtcttga tcagcagcga aaccgcaaag acaagcagga agcggaaaaa
     1441 acccggctgc gccttgaaga aaaaatcgct gtggctgtgg cccggaacgg gcgcaagcgg
     1501 ctgcgtgttg atcacgtccc attcattcgg aaaaagaaaa aacgggatca ggatgatcac
     1561 gatgaaacag agcaggctca ccaccgcgaa cttcgcgtac gacttcgtaa aatacaaccg
     1621 cggaataagc acatagaaat tgaggtagaa ataaccgatc gccagcgtat agctgaccag
     1681 gtctttgaga aacgaccgtg tggacaacac gcggaatgaa aaatcgaaat ccggggaaaa
     1741 gatgaccggc agcgacagga agcccgcaca ggctatcaca tgcagcgcga tctttccggc
     1801 tttgctgtcc attgttgtgt caagataacc aagttaatgc gctcgtcaaa acaaggtaaa
     1861 acaaagtgcg gcggaataga atgtttcgcc ggggaacctc gccgcaaatc ccgggccgcc
     1921 gggtttcaaa cggcaaacga tatattacca atcaaaaact gtttcagagc ctgagattat
     1981 cctgctcatc cagctcccag aaaggattga acgccacctc gaaaagatga ccgtccggat
     2041 cgcggaaata accgctgtat ccgccccagt acactttctg cgcaggtttt acgatgaccg
     2101 ccccgaggct ttccacctgc ttcatcactt cgtccacttc tttttccgat ttcgcgttgt
     2161 acgagatgct cagcccggcg aacccgcatt tttcatccgg caccgtcgcg tccttcgcca
     2221 gctcttcgcg cggatgaagt gcgagcgtca ttccgccaag cggaaaaagc gcaagcccgt
     2281 cgacgctggc tttcgaacgt ttccatccca ggcctttttc gtaaaaatca accgcccttt
     2341 cgaagtcact tacaccgagc gtaatgagat tgagtttctg tcgcatgaaa cgaatgtagg
     2401 aagaatgaga cttgcccgcc ctgcactttt cgtcaatacg gtttatcttg cggtccttcc
     2461 ccgttgaaag aatgtccgcc acgaaactcc tacccgaact cagcgaagat catccacgcg
     2521 ctcttccgtg ggtaatgctg ctgtgcgcgc tcggactttt cgcgtacgtc gccatccgtt
     2581 ccgccacgat cgacattacg atcgacgaag cgtattcctg gaaattcttt tcgtacggcc
     2641 cccatctcta ccctcagaaa tacgacgtca ccaccgcgaa cgatcactgg ctcaacagct
     2701 ggctcgtgca gctgacgggg ggtgttttcg gtccgcgcaa ctgggcgctg cgccttccca
     2761 acgtgctggc gttcgccgtt tatctttttt tcacggcgcg cttcgtgctt tcgttccggg
     2821 cccggtggta cacgctcgcc gctttcctcc tgctgaacgt tcatccttac ctgctcgatt
     2881 ttttctcggc ggaacgcggg tacggacttg ccttcggggc catgtcggcc tgcatttttt
     2941 ttcttttccg cttttccggt tcgctcgccc ggaaggacct gtgttttttt cttcttgccg
     3001 gttgtctcgc ggtgctggca aattttgcgg tgatcagctt ttttatggtc gcgctgggaa
     3061 tggccgtgct gctgacgctg ctttcgaaaa atgcggacag gaagaaactg cttcttctta
     3121 cggtttgcgt ttccgttccg tttctcgcga tcgtcgtccc tcacctgttc cacatgcagc
     3181 attccggcgc gctctattac ggagaaaact cgctgtggga agggacggcc aaatccgttt
     3241 tcaccagcct gctt
//
LOCUS       DASTSF010000375         7205 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1141539,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000375 DASTSF010000000
VERSION     DASTSF010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 7205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7205
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1141539"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..829
                     /locus_tag="VFU15_10700"
     CDS             <1..829
                     /locus_tag="VFU15_10700"
                     /inference="COORDINATES: protein motif:HMM:NF040240.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718296.1"
                     /translation="VNTFEKMVDTAGYYAASLPGHPDHVIIGMNSIFFSAKDSSRCLP
                     TDTSSGHAELNWIRRQLNSCREKKMKVWFTCHIPPGVNIKSTADGMQKTGKDCPKKVK
                     MMWKEDYNTEFLALENEYRDVITGGLAGHTHMDDFRVITDAAGKPVSFFHINPSISPI
                     DGNNPGFQLFTYDPGTMQMLDFVTYAFGGPGDAPGPDWKKEYDFDTAYGVSALDANGF
                     YDAAKTICSHGKKQKDYEAYYEMGSKQIKLEPWDAYACGFTHLDRAGFAGCACAKNK"
     gene            complement(842..1537)
                     /locus_tag="VFU15_10705"
     CDS             complement(842..1537)
                     /locus_tag="VFU15_10705"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEU4718297.1"
                     /translation="MKRITFALAACFALSSCHAEKMKDNPGDSGAVRFVALGDSYTIG
                     EGATWETSWPYLLAKSMNEKGKKVKLVANPSRTGWTTQDLIDNELPVFDTCRADVVTL
                     LIGVNDWVQGVSADDFKKNLVYILDHVQAKLEDKTKILLVTIPDFGVTPTGKFYSGGR
                     DISAGITQFNDIIRDEAKKRNLPLVDIFPVSKDMGKDSSLVAKDGLHPSAKEYAIWET
                     LILPEFEEMINDK"
     gene            complement(1750..2583)
                     /locus_tag="VFU15_10710"
     CDS             complement(1750..2583)
                     /locus_tag="VFU15_10710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718298.1"
                     /translation="MQTISWKNFTIRTDFHPAMPAGSLPGIPPAYNQWNPANVQVVNN
                     NLQLSLQRNSPTIWQGQHVWAASEAVVQNSLGYGTYCCTFRIVDASGKNAWAQFSTLN
                     ASPNTSTIFGVFLYDAVGTGGSNPNCEIDIIEVGFQNQPNNGQGWIGQQPGGPALNNA
                     QFVIQPWDAGTPNQPDWNMVKRINIDTASIPDPGDVTVICQWTAAGAPVTYYLAYGAY
                     TSANFPFATAMKYTTPVAANSYIPALNSNMRLHLNLWPYGGPTTNAPVFVQVSNLEIP
                     S"
     gene            complement(2896..3669)
                     /locus_tag="VFU15_10715"
     CDS             complement(2896..3669)
                     /locus_tag="VFU15_10715"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU4718299.1"
                     /translation="MNYIFSFLRKTSVITELEMRKIFHDPTEIVTRAVQPALWLLIFG
                     QVFARVHAFSAQTNYIDFMVPGILAQSVLFISIFSGIAIIWERDLGLIHKLLATPTPR
                     ASIVLGKAVSAGLRTLPVVIIIYILAVLLHVRIEWNPFSLLLVFFMIILGGIFFSTFS
                     LVIACLVKTRERFMGIGQLLTMPLFFASNAIYPIDIMPAWLKTLSHVNPLTYMIDALR
                     ALMITVPSAFGIGFDFAVLLCASVVMITAGSLLYRRVIT"
     gene            complement(3676..4473)
                     /locus_tag="VFU15_10720"
     CDS             complement(3676..4473)
                     /locus_tag="VFU15_10720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008595744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU4718300.1"
                     /translation="MSILETNNLTRSFGSFTAVDRVTMKVEKGEAFALLGPNGAGKSV
                     TLKMLTTLLPPSSGDAVINGFSIKSQPQKIRPIIGYVPQMLSADGSLTGYENLLLFAK
                     LYDIPYKERKTRVYEMLEFMGFSEHADKLVREYSGGMIRRLEIAQAILHNPPLLFLDE
                     PTTGLDPLGRNMVWEHIKALQKKYDTTIIMTTHMMEEAEHLCSHAGLMSRGKLAASGS
                     VRELKSSIGKNEATLEEVFIHYTADNLEPGDNYRQVQRERRTAKRLG"
     gene            complement(4508..4711)
                     /locus_tag="VFU15_10725"
     CDS             complement(4508..4711)
                     /locus_tag="VFU15_10725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718301.1"
                     /translation="MKKKKTTKEQPEKLPEEVPAEPKPEIPQPQPEIWPLPEKEPLPV
                     SPPEIEPAEEPEPEEPIEPGTEE"
     gene            complement(4723..4947)
                     /locus_tag="VFU15_10730"
     CDS             complement(4723..4947)
                     /locus_tag="VFU15_10730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718302.1"
                     /translation="MKRLSQPTGVLFFCNNRNINAMTFNPNLISNWPVISGMLKNITR
                     NYPMLLKMKLNAGKGELTPTEVRPDLHPAR"
     gene            5287..5922
                     /locus_tag="VFU15_10735"
     CDS             5287..5922
                     /locus_tag="VFU15_10735"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010480307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU4718303.1"
                     /translation="MIKTETFDRHPAEYDAWFDKHKAVFASEVEALRNMLPPGDSHGI
                     EVGLGTGRFAVALGIKEGVEPAADMRELALARGIEVMDATAEHLPYKDLHFDFVLMVS
                     CINYLDQPEDAFREAYRVLKHGGRLVVGTIEKESVIGKSYEARRRQSLFYHQAIFYPT
                     GKLVERLQNARFGHFEFSQTLFHPLEEIQSFELSRPGYGEGSFVVIKAMKK"
     gene            5919..6383
                     /locus_tag="VFU15_10740"
     CDS             5919..6383
                     /locus_tag="VFU15_10740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018477567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HEU4718304.1"
                     /translation="MKAAEKIKTLEVKSPAFSDRTIIPPVYTHDGRNINPPLTIGNIP
                     HETKSLALIIEDPDAPRKTSFVHWLMWNIPPAPEIVEDSVPGFQGKNDFGNLRYDGPA
                     PPSGTHRYFFKVYALDSLLKLKAGASRSELEKTMATHIIGYGDLTGLYGRAL"
     gene            complement(6940..>7205)
                     /locus_tag="VFU15_10745"
     CDS             complement(6940..>7205)
                     /locus_tag="VFU15_10745"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEU4718305.1"
                     /translation="VHVYPNPSLDGNVTLGFDVKTDENIGVKIFSADGRLVYTDEIFC
                     PADSHTALPVRLDEATAAAGYYILQVTSSTAILRQRIVVMRNH"
BASE COUNT         1834 a         1764 c         1808 g         1799 t
ORIGIN      
        1 ggtgaacact tttgaaaaaa tggtcgatac tgccggctat tacgcggcaa gccttcccgg
       61 tcatccagat catgtgatca tcggcatgaa ctcgatcttt ttttcagcga aagattcgag
      121 ccgttgcctt ccgacggata cgagctcagg gcatgcggag ctgaactgga tccgccggca
      181 gctgaacagt tgccgcgaaa agaaaatgaa ggtgtggttc acctgccaca tccccccggg
      241 cgtcaatata aaatcgactg cggacggcat gcagaaaaca gggaaggact gcccgaaaaa
      301 agtgaagatg atgtggaagg aagattacaa caccgaattt cttgcgctgg aaaacgaata
      361 ccgcgacgtc attaccggcg gactcgcagg ccacacgcac atggatgatt tccgcgtgat
      421 cacagatgcg gcgggaaaac ccgtctcgtt ctttcacatc aatccgtcca tcagtcccat
      481 cgacgggaac aatcccggct tccagctttt cacgtacgac cccggaacga tgcagatgct
      541 cgatttcgtg acctacgcgt tcggcggtcc cggcgacgca cccggcccgg actggaaaaa
      601 agaatatgat tttgacacgg catacggtgt aagcgcgctc gatgcaaatg gtttttacga
      661 tgcggcgaaa acgatctgct cccatggaaa aaagcaaaaa gattacgagg cttattatga
      721 aatggggagc aaacagataa aactggaacc gtgggatgcg tatgcgtgcg gattcacgca
      781 cctggataga gcgggctttg caggctgcgc atgcgccaaa aacaaatgac ccgccggttc
      841 atcatttatc atttatcatt tcttcgaact ccggcaggat cagcgtttcc catatcgcgt
      901 attctttcgc ggaaggatga agtccgtctt tcgcaacaag cgagctgtct tttcccatgt
      961 ctttcgaaac gggaaaaata tccacgagcg gaaggtttct ctttttcgct tcgtcgcgga
     1021 tgatgtcgtt gaactgcgtg atgcccgcgg aaatatctct tccgcccgag tagaatttcc
     1081 cggtgggcgt gacgccgaaa tcagggatcg tgacgagcag gatctttgtt ttatcctcca
     1141 gcttcgcctg cacgtgatcg agaatgtaaa cgagattctt tttgaaatca tccgcggaaa
     1201 cgccctgcac ccagtcgttc acacctatga gcagcgtcac cacatccgcg cggcacgtat
     1261 cgaaaaccgg cagctcgttg tcgatcaggt cctgcgtggt ccagcccgtg cgcgaaggat
     1321 tggcgacgag cttcaccttc tttccttttt cgttcatgct tttcgccagc aggtacggcc
     1381 atgaagtttc ccacgtggcg ccttcgccta tcgtgtagga atcgccgagc gctacgaaac
     1441 ggacagcgcc tgagtcgccg ggattgtctt tcattttttc agcgtggcag gatgataatg
     1501 caaaacaggc cgccagcgca aacgtaattc ttttcacaga ccgatatacg gaaaccggct
     1561 tcttccggat ttgctggcac gggcgaatat cctctttcgc atttttgatc tttctgcggc
     1621 cgggaaacat agacctggag aaaaaacatt gaaccgcaat tacacagcag cggaccgtta
     1681 tcattttacc ggccacaaaa aaaacaggcg cccgtgagcg cccgttccat tttccaaaat
     1741 cgttgaatgc tacgaaggaa tttccaggtt cgatacctgc acgaagaccg gcgcattggt
     1801 cgtgggaccg ccatacggcc acaggttcag gtgcaggcgc atgttgctgt tgagcgcagg
     1861 gatgtatgaa ttggccgcta ccggcgtggt atatttcatt gcagtggcga aaggaaaatt
     1921 cgcagacgta taagcgccat acgcgaggta atacgtcacc ggcgcgccgg ctgctgtcca
     1981 ctggcagatc acggtcacat caccgggatc aggaatgctg gcggtatcga tattgatgcg
     2041 cttcaccatg ttccagtcgg gttgattggg cgttcccgca tcccagggct ggatcacgaa
     2101 ctgtgcgttg ttcagcgcgg gtccgccggg ctgctgcccg atccatccct gcccgttgtt
     2161 cggctggttc tggaaaccca cctcgatgat atcgatctcg caattcgggt tggagccgcc
     2221 cgtaccaacc gcgtcgtaca ggaaaactcc gaaaatggtg ctggtattgg gagacgcgtt
     2281 cagcgtgctg aactgcgccc aggcattttt cccgcttgcg tccactatcc tgaacgtgca
     2341 gcagtatgtt ccatagccca ggctgttctg taccaccgct tcagacgcag cccacacatg
     2401 ctggccctgc catatcgtgg gtgaattccg ctggagtgaa agctgcaggt tattgttcac
     2461 cacctgtacg ttcgcaggat tccattggtt gtaggccggc ggtatcccgg gaagacttcc
     2521 cgcaggcatc gcggggtgaa aatcggttcg gatcgtaaaa tttttccagc tgatggtttg
     2581 cataacaatt gatttttgag tgaataaccg ggaagcgttt gtcgtgtaac gacaacagaa
     2641 atacgatttt ttttgaaaat accatcaata aaaattttat cgctgcttcg tttcttcggt
     2701 aggagaaggc aaaaatggtg gcggggtcac atgaggtgcc cgaccgtaag gagcagcatg
     2761 gcgttggatt atttgaacag tccttatcgt atgcgttacc gttcgaccga aaagtttaac
     2821 caaaaggttt ttaatttccc gggcgaaaaa ccatgctcac gccattgcgg atttggaaat
     2881 acccgcctgc gccacttagg taatcacccg ccggtagagc aggctgcctg cagtgatcat
     2941 taccacggag gcgcataaca gcacagcaaa gtcaaaacca ataccaaatg ctgacggcac
     3001 ggtaatcatc aatgcgcgca gggcgtcaat catatacgtc agcggattaa catgtgataa
     3061 tgttttgagc catgccggca tgatatcgat cgggtaaatg gcgttgcttg caaagaaaag
     3121 gggcatggtc agcagctgcc cgattcccat gaatcgttcg cgtgttttga caagacaggc
     3181 gataaccagg gagaaagtcg aaaagaaaat tccgccgaga atgatcatga aaaaaacaag
     3241 aaggaggctg aacgggttcc attcaattct tacgtgaagc aatacggcaa gaatgtaaat
     3301 aatgatcacc accggaagcg tgcgaagccc ggccgaaaca gcctttccca atacaatgga
     3361 agcacgtggc gtgggtgtgg caagcagctt gtgaattaat ccgagatcgc gttcccagat
     3421 aattgcaatt cccgagaaaa tggagatgaa gagcacactt tgagcaagaa ttccgggcac
     3481 catgaaatca atataattgg tttgtgcgga gaacgcatgc acgcgggcga atacctgtcc
     3541 gaagatcagc agccataatg cgggttgcac agcgcgcgtc acgatttccg ttggatcgtg
     3601 aaatattttg cgcatttcca gttcggtaat aaccgatgtc tttcgcagga aagaaaaaat
     3661 gtaattcata attttttatc ccaggcgttt tgcggttctt ctttctcttt gcacctgccg
     3721 gtaattatca cctggttcaa gattatctgc cgtataatga ataaaaactt cttccagcgt
     3781 tgcctcattt ttcccgatcg atgatttcag ttcacgtaca gaaccgcttg ccgccagttt
     3841 cccgcgcgac ataaggcccg catgcgagca caggtgttcg gcttcctcca tcatgtgcgt
     3901 ggtcatgata atggtcgtgt catatttctt ctgcaatgct ttgatgtgct cccaaaccat
     3961 gtttcgtccg agaggatcca ggccggtggt aggttcatcg agaaataaca acggtggatt
     4021 atgtaatatc gcctgcgcaa tttcaagcct gcggatcatg ccgccggaat attcgcggac
     4081 aagtttatcg gcatgttcgg aaaaccccat gaactcaagc atctcatata ccctggtctt
     4141 gcgctctttg taaggaatgt cataaagttt tgcaaacagg agcaggtttt catatcccgt
     4201 cagcgagcca tcggccgaaa gcatctgcgg aacatacccg attattggcc ggattttttg
     4261 aggctgcgat ttgatgctga acccattgat gaccgcatcg ccggatgaag gcggaagcag
     4321 cgtagtcagc atttttaggg taacactctt tcctgctccg ttcgggccaa gcagggcgaa
     4381 agcttctcct ttttccactt tcatcgtcac ccggtctacc gcagtaaatg acccgaatga
     4441 acgggtcagg ttatttgtct cgagaatact catgtgcaga tttgaaaagc tctttattca
     4501 ggtgatttta ttcttctgta cccggttcaa tgggctcttc cggctccggt tcctccgccg
     4561 gttcaatttc cggaggggaa acaggcaacg gctccttttc gggtaaaggc catatctccg
     4621 gctgcggctg aggaatttcc ggcttcggct cagcgggaac ttcttccggc aatttttctg
     4681 gttgctcttt tgtcgttttt ttctttttca tcctggaaac tatcatctgg ccggatgtaa
     4741 atcaggacgc acttctgtcg gcgttaattc tcctttacct gcatttaatt tcattttgag
     4801 cagcatcgga taattccggg taatgttttt aagcatgccc gagataaccg gccaattact
     4861 gatcagattc ggattaaatg tcatggcatt tatatttcgg ttattacaaa aaaataaaac
     4921 tccggtgggt tgcgaaaggc gtttcatcag ttccggcaat gatttttttc atcactggta
     4981 aatgaacaaa agatcacgga tagcattgac caattccata gataacggaa actgccggat
     5041 ttacttttgc ttatatcttc ttttgggatt ggttggcgga gtaagcttgc aggtgctgac
     5101 gctttaacag gcgtgaatcc tgcgggtacg gaacacagca gaaaagctgt tatctcccgg
     5161 ccagcagtcc acactgagga aggaaaaagg cagaataacc gcagtaaaat gcgccagact
     5221 gccgactatc cggggatggt tcgttagcgg accatccttg tttctaaact tcgaaaatct
     5281 aatcacatga tcaagacaga aacatttgac aggcatccgg cagaatatga cgcatggttt
     5341 gataagcata aagcggtatt tgcttcggaa gtagaagcgt tgcggaatat gttgccaccc
     5401 ggagacagtc acggtatcga agttggcctg ggaacgggcc ggttcgccgt cgcattagga
     5461 atcaaagaag gagtagaacc ggcagcggac atgcgcgaac tggcgctggc gcgtgggatt
     5521 gaagtgatgg atgccacagc cgaacatttg ccctataaag atctgcactt cgattttgtg
     5581 ctgatggttt cctgcatcaa ttatctcgat cagcctgaag atgcgttccg ggaagcatac
     5641 cgtgtgttaa agcacggagg ccggctggta gtgggaacaa tagagaaaga aagcgtgatc
     5701 gggaaatcat acgaggcccg caggcggcaa agcctttttt accaccaggc aatattttat
     5761 ccgaccggca agctggtgga acgtttacaa aacgcacggt tcggtcattt tgaattttcg
     5821 caaacacttt ttcatccgct tgaggaaatt cagtcgttcg agctttccag gcccggttat
     5881 ggggaaggtt cttttgttgt aattaaagcg atgaagaaat gaaagcagcg gaaaaaataa
     5941 aaacgctcga ggtgaaaagc ccggcgtttt ctgaccgtac aattattccc ccggtctaca
     6001 cgcatgacgg gcggaacatt aacccgccgc tgaccattgg gaatatcccg catgagacga
     6061 aaagccttgc actgatcatc gaagatccgg atgcgccccg caaaaccagt tttgtgcatt
     6121 ggctcatgtg gaatattcct cctgctcctg aaattgttga ggacagcgta cccggattcc
     6181 aggggaagaa tgatttcggg aacttgcgtt acgacggtcc tgcgcctcct tccggcacgc
     6241 atcgttattt tttcaaggtt tacgcgcttg attccctgtt gaaactgaaa gcaggcgcaa
     6301 gccggtccga actggaaaaa acgatggcta cccacatcat tggatatggc gacctgacag
     6361 gtctttacgg aagagcattg taaaaaataa ttatgaagcc gattaacatc cctgttccgc
     6421 tcccggaggt ggcactgagc gccggcccgg ctatttcatt cggggcaaat gtacccgttg
     6481 tgttctccca cggcagcgga agcagctggt taagtccggg aaatcgtttt attgcagaaa
     6541 gagcattcaa tacgatcacc aacagattgc ctgctgaaaa caggatgaag taaaatctta
     6601 tcttgatata gctctttcaa aaacgggcgt tctgcccgtc agctgaagca cccgcaagta
     6661 ttatctatat tcccggcatg ttacgtgtgt ccggcagcaa accttactgc cttttgaggc
     6721 ttagcttctt acccccattt gtcagtattc aaagattctt aaaacccggc caggatttaa
     6781 atcctgaccg ggaattattg caatgctttt aatcagtgcg atggatcatc aatttattaa
     6841 attgactttc accgtttcca ataaatttaa acaaataaag aggaccttgc gcaatcaata
     6901 aagcatgttt atacctgtcc taaaccggac tgatccgcct caatggttcc tcataacaac
     6961 aatcctctgt cttagaatag ccgttgagga agtcacctgc aaaatataat acccagctgc
     7021 agcagtcgcc tcatcgagtc ggacgggtag tgcagtatga gaatcagccg gacagaaaat
     7081 ttcatcggta tacacgagcc tgccgtcagc ggaaaaaatt ttcaccccga tattctcatc
     7141 tgtttttaca tcaaagccca gggttacatt tccgtcgaga gacgggttcg gataaacatg
     7201 tacac
//
LOCUS       DASTSF010000376        12525 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1145541,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000376 DASTSF010000000
VERSION     DASTSF010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 12525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12525
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1145541"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..641)
                     /locus_tag="VFU15_10750"
     CDS             complement(<1..641)
                     /locus_tag="VFU15_10750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718306.1"
                     /translation="MKQRLPAFLLIFLFPVLLSAGNKYWTAGSGNWNDPAHWSESPNG
                     KGGAGIPSVNDDIFIGTDVSSEDADTVTIDGKANCRNFNYGDGVNANKIVLRGNGEIS
                     VHGSLLFTHAFNDLFTGTWNFSDPAAGVRDNSLPTITTNNHVFSGKLVFISGPGPQRK
                     NIFLANDNLFVNNSIGINSNSELILSDHTELVANEIILNGTGSNVTSRPGSKV"
     gene            797..1003
                     /locus_tag="VFU15_10755"
     CDS             797..1003
                     /locus_tag="VFU15_10755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718307.1"
                     /translation="MKSKQEESPGADPDIPVYLKIGPHFILPSLIRSVSRFGKGTKII
                     FASGNEVVVNVKYEKVAEVMKGSG"
     gene            complement(1019..1636)
                     /locus_tag="VFU15_10760"
     CDS             complement(1019..1636)
                     /locus_tag="VFU15_10760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEU4718308.1"
                     /translation="MKSFSKYLSVQFHPLLMVCYAAAIYLYLLPLDGEIIDSRIRLIL
                     MGFLLCGTVFIPLFLTMVILRFSPAKSMQMTEPKERNWPLLFTAVVYFFCYFSLTAKQ
                     VPVFVKLFLLGSIAGILLSLLINLRWKISLHMTGIGGLCGAICAILLLAGIHMPWLAA
                     GSFLLAGMLGTARLYLDAHSPQQVAAGFFLGFALEFSTVFFLLEH"
     gene            complement(1640..3124)
                     /gene="rpoN"
                     /locus_tag="VFU15_10765"
     CDS             complement(1640..3124)
                     /gene="rpoN"
                     /locus_tag="VFU15_10765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010601731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HEU4718309.1"
                     /translation="MLKQSLQQKLLQKLSPQQIQLMKLLQLPTVALEQRIKEELEINP
                     ALEEGPETDEDERKDDLSDDDDGVFEDSASEDGESDERKKDDDFALGDYMDEDEGYSY
                     RERTNNSGPDDERREMPLTQGPGFLEQLLSQLGLQPLDDHHYQVGAYLIGNIDEDGYM
                     RRELSAVVDDLAFSQNITTNEAELNEMLTLIQTFDPPGVGARDLQECLMLQLQRKNPK
                     TAEVVLAMRVVSEHMDEFSKKHYDKIARKLGVDDDSLKRVIREITHLNPRPGNAAADS
                     ARVVQDVVPDFTLINDDGRLDIILNERNMPDLRVSAMYSQMLAEYARSKEKSSREAVS
                     FIKQKIDSAKWFMDALRQRHQTLYVTMKAILDYQYDYFLEGDETQLKPMILKDIADRV
                     GLDISTVSRVANSKYIQTPFGTFLLKSFFSESLSTDSGEEVSTREVKKILSDCISAED
                     KRKPLTDDRLAKILKEKGYNIARRTVAKYREQLDIPVARLRKEL"
     gene            complement(3241..4134)
                     /locus_tag="VFU15_10770"
     CDS             complement(3241..4134)
                     /locus_tag="VFU15_10770"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEU4718310.1"
                     /translation="MAYNYTNLVFKGGGVLGIAYAGAIKVLEDNGILQQVRRAAGTSA
                     GAITAALVCLKYSAAEILHEVSATNFKSFEDGLDPLRVATKYGLFKGDAFLSWMQQRL
                     IDKGFSATATFTDLKNKGMSDLHVFASDLNTQNLREFSFATTPGVPVAYAVRASMSIP
                     LFFNAWQFPGNNPDNHLYVDGGMVYNYPVRTFDQNGVNPATLGFFLSNLNAPAIADTL
                     DYNHLVQYVRNTFDTIMNAQNINFRNDPSDEKRTVMIDNLGISATNFGLTQQQEQDLY
                     NSGVKYTTAFLQPDQFTTPVA"
     gene            complement(4228..4758)
                     /locus_tag="VFU15_10775"
     CDS             complement(4228..4758)
                     /locus_tag="VFU15_10775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718311.1"
                     /translation="MRLLPYVLPAVLLLCSCDRKIGVYKMENNADEISVRIPGDTTAS
                     VDVRASDDEGKLQIMIARYGRQRFLASLEAAGAELVAGEKTVAADKAITLIDLDKGEV
                     KSSDYSEIRTAVQKLPEDTLNTAFGITTFLDYDHPGDLPATFSVKLHIKSPAGTKDTT
                     LVFTRSSATIRAGRPY"
     gene            complement(4851..5594)
                     /locus_tag="VFU15_10780"
     CDS             complement(4851..5594)
                     /locus_tag="VFU15_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HEU4718312.1"
                     /translation="MNLTLKVWRQKNANDKGGFVTYNVTDISPDMSFLEMFDVLNERL
                     ISEGKEPIAFDHDCREGICGMCSMYINGRAHGPWEGTTTCQLHMRAFKDGDSITVEPW
                     RAKAFPVIRDLVVDRSAFDKIQAAGGFVSVNTGNAQDANSIPVPKDDADDAFAAAACI
                     GCGACVAACKNSSAMLFVSAKVSQFALLPQGQVERVDRVKNMVKEMDEMGFGNCTNTG
                     SCEAECPKEISLENIARLNREYLMANLKK"
     gene            complement(5673..7652)
                     /locus_tag="VFU15_10785"
     CDS             complement(5673..7652)
                     /locus_tag="VFU15_10785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010599640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HEU4718313.1"
                     /translation="MAKLESKIPEGALDQKWTTYRSTVPLVNPANKRSLEIIVVGSGL
                     AGASAAASLAELGYKVKVFCFQDSARRAHSIAAQGGINAAKNYQNDGDSTYRLFYDTI
                     KGGDYRSREANVHRLAEVSANIIDQCVAQGVPFAREYGGMLSNRSFGGVQVQRTFYAA
                     GQTGQQLLLGAYSALQRQVGLGTVKMYARHEMLDVVLVDGKARGIIARDLISGKLERH
                     SGHAVLLCTGGYGNVFYLSTNAMGSNVTAAWKAHKRGAFFGNPCFTQIHPTCIPVTGD
                     HQSKLTLMSESLRNDGRIWVPKKAGDTRKGNEIPEDERDYYLERRYPAFGNLVPRDVA
                     SRAAKERCDAGFGVGASKMAVFLDFASAIERYGKQQANKLNEENPSAARIRELGKEVV
                     KEKYGNLFEMYQKITDENPYEVPMRIYPAVHYTMGGLWVDYELMTTVPGMYALGEANF
                     SDHGANRLGASALMQGLADGYFVIPYTIGSYLSGMIRDKGVPTDHPAFAETEKAVQDT
                     LDKLMNIKGTKSVDHFHKKLGKIMWDKCGMARNAKGLQEAITEIRALREEFWKDVRVP
                     GGQNEFNPELEKAGRVADFLELGELMCMDALQRNESCGGHFREEYQTEDGEAKRDDAN
                     FMYVAAWENKGDNKFEMHKEVLKYDVVHPTQRSYK"
     gene            complement(7737..8432)
                     /locus_tag="VFU15_10790"
     CDS             complement(7737..8432)
                     /locus_tag="VFU15_10790"
                     /inference="COORDINATES: protein motif:HMM:TIGR02046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b subunit"
                     /protein_id="HEU4718314.1"
                     /translation="MNKFLTSSIGKKTVMALSGLFLILFLLEHIYGNVLLYFGDGGAA
                     FNEYSHDAVRNILIRIIEVVLFLAIAAHVAQALILTGENRRARPVKYAVNGVSQTSPW
                     VSRNMLLTGGVVFFFIVVHLYNFFVPYRIMGTVGHGDEQLTIAEKVAEALGNPLYAGL
                     YLAGVIFLALHLSHALASAFHTLGLDNKKYASTWKMVSTCFAVLMGIAFASFPILFYG
                     AKLMHKDLLHWNM"
     gene            complement(8598..9497)
                     /locus_tag="VFU15_10795"
     CDS             complement(8598..9497)
                     /locus_tag="VFU15_10795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718315.1"
                     /translation="MSAIRNYFTVAPPDIGVKNYRFYVVNHIAGTVGGLTHFSWLVLF
                     GITHTYPLFYFNFFSVALFVFFIWLNKRGHHLTALLMGISEVIAHQVLGVYVLGWDAG
                     FQYILIIASMLLFLMPKGNTLVKYIFALLCVAGFLFIELVLRKSAPLYTIDKTVLLVL
                     DISNITLSFFFLFIWAGFYNYAIFETEKALEERTEEVARAEKKAAVGTLTAKIAHEIQ
                     NPLNFVNNFSEISAELVNEIVHAGSEEERARLLAQLKDNLEKIGAHGKRADQIVKSMF
                     LHLRERKGESPFGEKDADLKEEA"
     gene            complement(9779..10528)
                     /locus_tag="VFU15_10800"
     CDS             complement(9779..10528)
                     /locus_tag="VFU15_10800"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEU4718316.1"
                     /translation="MIKTIIIDDEKGNVGSLKELLNAYCRQVAVVATAENVADAIRLI
                     DAHKPELIFLDVELKKELGFDIFRHYPDPFFQVIFTTAHEKYALRAIKSSALEYLLKP
                     VDYRELVRAVEKFEKQQHVTMSQKKISVLLENLGNDSQSFSKIAIPSSDGYSFVNTGE
                     IIYCEADMNYTNVVTNKGEKILSTRNLKEFEEMLNPQVFFRCHKSWLINLNFIKKYSR
                     TDGTRVLMANDAWIDISVRKREEFLKLFGKF"
     gene            10690..11490
                     /locus_tag="VFU15_10805"
     CDS             10690..11490
                     /locus_tag="VFU15_10805"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEU4718317.1"
                     /translation="MKKFLLALVAALPVVLGAQTVSYPDTVLDFSTEYFPSDSGCTAN
                     WSACRALGAPDVYPAWGDNSNAWTATSTTGTSSPVPGREWIVLGFTAPVTADSVIVYE
                     TCSRGGTDTVYVRDASSGNWTVIWDTTAYDGNIPDSCNAIHIGLSSSMNIDAVRIAID
                     NAHYAFWNEIDAVGVISSGTTGVAAGTGISRPVIFPNPGNGTFTLTLPAGWKSCSMEI
                     FDMTGRLCFAQYGIASSTLQIETKLQPGTYPVKLASGEEQQVQMIVVQ"
     gene            11515..>12525
                     /locus_tag="VFU15_10810"
     CDS             11515..>12525
                     /locus_tag="VFU15_10810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718318.1"
                     /translation="MKKVIFTSAILLASLAAGAQGLANVWNGFYNQVSPVITTDVQCS
                     WVDAAGNLYTAGMQQSDVDADIATANVTTLGNSGSNYFYFCKYNSSGVLQLAKQLNSS
                     SVNTTDMAGGIVSDASGNIYVTGHFTGTVDFDPGAGTSAKTSTSGGSGFLAKYSSTGA
                     LLWVQNFGNIYTYPQDIALNPVNGNVWIGGYLIANSSIGYADMDPGAGTASNFGNSNG
                     SVGFYCAYSAAAGSYIANTNYAICSTTLNYDCSVNAICFDGTTSNIYLTGSFRGTIYP
                     DNSTSGGPPITTSATKAMFTIRQDIVNTNVNASYDYFDGSPVNEGTDIAVDASGNIFT
                     AGY"
BASE COUNT         2802 a         3269 c         3475 g         2979 t
ORIGIN      
        1 atcactttcg agcccggccg gctcgttaca ttgctgcccg tgccattgag aataatctcg
       61 ttcgcgacaa gctcagtatg atcagagagg ataagttcgg aattggaatt tatcccgatg
      121 ctgttgttta caaaaagatt gtcgttcgcc agaaaaatat ttttacgctg ggggcctggt
      181 cctgaaataa aaacaagctt tccgctgaat acatgattgt tcgtggtgat cgtaggcagc
      241 gaattatctc tcacgcccgc tgcgggatcg gagaaattcc atgttcctgt gaaaagatca
      301 ttgaacgcat gtgtgaaaag aagagagccg tgcacggaaa tttccccgtt gccccgcagc
      361 acgatcttgt tcgcgttaac gccgtcgccg taattgaaat tccggcaatt cgctttcccg
      421 tcgatggtaa cggtgtccgc gtcttctgac gaaacatccg ttccgataaa aatatcgtca
      481 ttcacggaag ggatcccggc gccgcctttt ccgtttggcg attcggacca gtgcgcagga
      541 tcgttccaat tgcctgatcc cgcggtccag tatttattgc cggcggaaag cagaacagga
      601 aataaaaaaa tcagcagaaa agcgggtaag cgctgtttca aaacaattgg tgtttaatgt
      661 gtttgccggt aaagttccgc gaatccagtt tgcccgaggt ttcgcgaaaa ctattaattc
      721 aacaaggttg ttaacagttt acgaatccgg ggtaggaatc gttaccttta catagcgaaa
      781 aataataaac ttcccagtga aatccaaaca ggaagaaagc cccggtgccg acccggatat
      841 tccggtttac ctgaagatag gtccgcattt tattctgccc tcattgatcc gttccgtttc
      901 ccgtttcggc aaggggacga aaataatttt tgccagcgga aatgaggttg tcgtgaatgt
      961 gaagtatgaa aaagtggccg aggtgatgaa aggttccgga taggctccct gcgcgtgatc
     1021 agtgttccag caggaaaaac acggttgaaa attccagcgc gaatcccagg aaaaatcctg
     1081 cggctacctg ctgcggcgaa tgcgcatcga ggtaaagccg cgcggtgccg agcattccgg
     1141 ccagcaggaa acttcccgcc gccagccagg gcatgtggat tccggccagc aataaaatcg
     1201 cgcagatcgc accgcataat cccccgatgc ccgtcatgtg aaggctgatc ttccagcgca
     1261 ggttgatcag cagcgaaagg agaattcccg cgatcgatcc gagaagaaag agcttgacga
     1321 aaaccgggac ctgctttgcc gtcagcgaga agtagcagaa aaagtaaacg actgcagtga
     1381 acagcagcgg ccagttccgt tctttgggtt cggtcatctg catcgacttt gccggactga
     1441 aacgcaggat caccatggtc aggaagagcg ggatgaaaac cgttccgcac agcaggaatc
     1501 ccatcaggat aagccggatg cggctgtcga taatttcgcc gtcgaggggc agcaggtaga
     1561 ggtaaatggc cgcggcatag cagaccatca gcaacgggtg gaactggacg gaaaggtatt
     1621 tcgagaaaga tttcacggat cagagctcct tgcgaagacg cgcaacgggg atgtccagct
     1681 gttcgcggta tttggctacg gtgcggcgtg cgatgttgta gcctttttct ttgaggatct
     1741 ttgccagccg gtcgtcggtg agcggtttgc gtttgtcctc ggcggaaatg cagtcggaga
     1801 ggattttttt cacctcgcgc gtggaaactt cttccccgct gtcggtggaa agtgattcgg
     1861 aaaaaaaact tttgagcagg aacgtgccga agggcgtctg gatgtatttg ctgttcgcga
     1921 cgcgcgaaac cgtcgagatg tcgagcccga cacggtcggc gatgtctttc aggatcatcg
     1981 gcttcagctg tgtttcgtct ccttcgagga aataatcgta ctggtaatcg aggatcgctt
     2041 tcatcgtcac gtagagcgtc tggtgccgct ggcgcagcgc atccatgaac catttcgcgg
     2101 aatcgatctt ctgcttgatg aacgaaacgg cttcgcggct gctcttctct ttgctgcgcg
     2161 catattcggc gagcatctgg ctgtacatcg cgctcacgcg aagatcaggc atgttgcgtt
     2221 cgttgaggat aatgtcgaga cgcccgtcgt cgttgatgag cgtaaaatcg ggaacaacat
     2281 cctgcacaac gcgtgcagaa tcggcggcgg cattccccgg gcgcgggttc aggtgcgtga
     2341 tctcgcgtat cacgcgcttg agtgaatcgt cgtcgacgcc gagcttgcgt gcgatcttgt
     2401 cgtaatgctt tttcgaaaat tcatccatgt gctcgctcac cacgcgcatg gcgagcacga
     2461 cttccgccgt cttcggattt ttccgctgca attgcagcat gaggcattcc tgcaggtcgc
     2521 gcgcgcccac gccgggcgga tcgaacgtct ggatgagcgt gagcatttcg ttcagctcgg
     2581 cttcattcgt ggtaatgttc tgcgaaaatg caagatcatc caccactgcg gaaagctcgc
     2641 gccgcatgta accgtcttcg tcaatattcc cgatgaggta agcgccgacc tggtaatgat
     2701 gatcgtcgag cggctgaagc ccgagctggg aaagcagctg ttcgagaaaa ccgggcccct
     2761 gtgtcagcgg catttccctc cgttcgtcgt cgggacctga attgttcgtg cgttcgcggt
     2821 aagaatagcc ttcgtcctcg tccatgtaat cgcccagcgc gaaatcatcg tccttcttgc
     2881 gctcatcgct ttcgccgtct tcggaagcgc tgtcttcgaa cacgccgtcg tcgtcgtcgg
     2941 aaagatcatc cttgcgttcg tcctcgtcgg tttccggtcc ttcttccagc gcgggattta
     3001 tctcgagctc ttccttgatg cgctgctcga gcgcaacagt gggaagctgc agcagcttca
     3061 tgagctgtat ctgctgcggg gaaagcttct gtaataattt ctgctggagc gactgcttga
     3121 gcataattca gttttgctcc cccaaaatac acattatctg aaaaagggaa tgatttttga
     3181 tgagcaaaag aaagccgttg tcttttccag gtaagacaac ggctttttca ttctgaaaaa
     3241 tcaggcaacc ggcgtcgtga actgatcggg ctgcaggaaa gcggtggtgt atttcacgcc
     3301 ggagttgtaa aggtcctgtt cctgctgctg cgtaagaccg aaattggtgg cggaaatgcc
     3361 gaggttgtcg atcatcacgg tgcgcttttc atcggaagga tcattccgga aattgatgtt
     3421 ctgcgcattc atgatcgtgt cgaacgtatt cctgacatac tggaccaggt gattgtaatc
     3481 gagcgtgtcg gcaatggcgg gagcgttcag gttgctcagg aaaaaaccga gtgtggccgg
     3541 attcacgccg ttctgatcga atgtgcgcac ggggtaatta tacaccattc cgccgtcgac
     3601 gtaaagatgg ttatcggggt tgttgcccgg gaattgccac gcgttgaaga aaagcgggat
     3661 agacatggaa gcgcgtacgg cgtaggcaac gggaacaccg ggcgtggtgg cgaagctgaa
     3721 ttcgcgcagg ttctgcgtgt tcaggtcgga ggcgaacacg tgcaggtcgc tcatgccttt
     3781 gtttttcaaa tcggtgaacg tggccgtggc ggaaaatcct ttgtcgatca gccgctgctg
     3841 catccacgaa agaaatgcgt cgcccttgaa gagcccgtat ttcgtcgcta cgcgcaacgg
     3901 gtcgagaccg tcttcaaacg atttgaaatt cgtggcgctg acctcgtgca ggatttcagc
     3961 cgcgctgtac ttcaggcaca caagcgccgc agtgatcgcg ccagcggaag ttccggcagc
     4021 acgccggacc tgctgcagga tgccgttatc ctcgagcact tttatcgcac cggcataggc
     4081 gatgccaagc acgccgccgc ctttgaaaac aagattggtg tagttgtaag ccatacagtt
     4141 tatttgtttc gcacaatata ccttttttta aaggcaggat tgataattgc aaaaggataa
     4201 cggagaaatg atgtgcgatg caggatatca gtaaggtctt cccgcgcgga tggttgctga
     4261 agaacgcgtg aacacgagcg tggtgtcttt ggtgccggcc ggagatttta tatgcagctt
     4321 cactgaaaat gtcgcgggca gatcgccggg atgatcataa tcgaggaatg tcgtgatgcc
     4381 gaatgcggta ttcagcgtat cctccgggag cttctgcacc gctgttctta tttcggaata
     4441 atcggacgac ttcacttctc ctttgtccag gtcgatcagg gtgatcgctt tatccgcggc
     4501 cacggttttc tctccggcca caagctccgc accggctgct tcgagcgaag caaggaaacg
     4561 ctgtcttccg taacgcgcga tcatgatctg cagcttccct tcgtcatcgg aagcgcggac
     4621 atccaccgaa gcggtcgtat cacccggtat gcggacggag atctcgtcgg cattattttc
     4681 cattttgtaa accccgatct tcctgtcgca ggagcagagc agaagaacag cgggaagaac
     4741 gtacggcagg aggcgcattt ttttcttttg aaaaagataa atcattattc ccataaaaaa
     4801 acccggcaac cgttgtccgg cggccgggta tcgttctcgg aacggaaaaa ttattttttc
     4861 aggttggcca tgaggtactc acggttgagg cgcgcgatgt tctcgagcga aatttccttc
     4921 gggcattccg cttcgcagct tcccgtgttg gtgcagttgc cgaaccccat ctcgtccatt
     4981 tccttcacca tgttcttcac gcggtcgacg cgttccacct gtccctgcgg aagaagcgcg
     5041 aactgggaaa ccttggcaga gacgaacagc atcgcggatg aatttttgca ggcggccaca
     5101 catgcaccgc agccgatgca cgcagctgcc gcgaacgcat cgtcggcgtc atctttggga
     5161 acgggaatgc tgttcgcatc ctgcgcattg cccgtgttga cggaaacgaa accgccggcg
     5221 gcctggatct tgtcgaacgc agagcggtcg acgacgagat ccctgatcac gggaaaagct
     5281 ttcgcacgcc agggctcgac ggtgatcgag tcgccatcct tgaaggcgcg catgtgcagc
     5341 tggcacgtgg tggttccttc ccagggtccg tgcgcgcggc cgttgatgta catgctgcac
     5401 attccgcaga tgccttcacg gcaatcgtga tcgaacgcga tcggttcttt tccttcgctg
     5461 atgaggcgct cgttgagcac gtcgaacatt tcgaggaaag acatgtcggg tgaaatgtcc
     5521 gttacgttgt acgtgacaaa cccgcctttg tcattggcgt ttttctgtct ccagactttg
     5581 agtgtgagat tcatacactt ttgaattttc cctgcgtccc tgcgtttctg cggcaattct
     5641 tccgccacgg aaacacggag gcacggagag atttacttat agctccgttg cgtcgggtga
     5701 acgacgtcgt atttcagtac ttccttatgc atttcgaatt tgttgtcgcc tttgttttcc
     5761 caggcagcga catacatgaa attcgcatcg tcgcgtttcg cttcgccgtc ttccgtctgg
     5821 tattcttcac ggaaatgacc tccgcacgat tcgttgcgct gcagcgcgtc catgcacatg
     5881 agctcgccga gctcgaggaa atccgccacg cggcccgctt tttccagctc aggattgaat
     5941 tcattttgtc cgcctggaac acgcacgtcc ttccagaatt cttcgcggag cgcgcgtatt
     6001 tccgtgatcg cttcctgcag tcctttcgcg ttgcgcgcca tgccgcattt gtcccacatg
     6061 atcttgccga gctttttgtg gaagtgatcc accgacttcg tgcctttgat gttcatcagc
     6121 ttgtcgagcg tgtcctgcac ggctttttcc gtttcggcga acgccgggtg atcggtggga
     6181 acacctttat cgcggatcat tccactgagg tacgaaccga tggtgtacgg gatcacgaaa
     6241 tacccgtcgg caagtccctg catcaatgcg gaagcgccaa ggcggttcgc gccgtgatcg
     6301 gaaaaattcg cttcgccgag tgcgtacatg ccgggaaccg tcgtcatcaa ttcataatcg
     6361 acccagagtc cgcccatcgt gtaatgcacg gcgggataaa tgcgcatcgg aacttcgtac
     6421 ggattttcgt ccgtgatctt ctggtacatc tcgaaaaggt tgccgtattt ttctttcacg
     6481 acttctttgc cgagctcgcg gatgcgcgcc gcagaaggat tttcttcgtt gagcttgttc
     6541 gcctgctgtt ttccgtagcg ttcgatggcc gaagcgaaat cgaggaacac cgccattttg
     6601 gaagcgccca cgccgaagcc ggcatcacag cgttctttgg ctgcgcgtga agcgacgtcg
     6661 cgcggaacaa ggttcccgaa agccgggtaa cggcgttcga ggtaataatc gcgctcgtct
     6721 tccggtatct catttccttt tctcgtgtcg cctgcttttt tcgggaccca gatgcgtccg
     6781 tcgttgcgca gcgattccga catgagcgta agcttcgact ggtgatcacc ggtaaccgga
     6841 atgcaggtcg ggtggatctg cgtgaagcag ggatttccga aaaatgcgcc gcgtttgtgc
     6901 gctttccatg cggcggtgac attcgatccc atcgcattcg tggaaagata aaatacgttt
     6961 ccgtagccgc ccgtgcagag caggacagca tgacccgaat gacgctcgag cttcccggaa
     7021 ataagatcgc gtgcgatgat gccgcgggct ttcccgtcga cgagaacgac gtcgagcatt
     7081 tcgtggcgcg cgtacatttt tactgtgccg agcccgacct gccgctgaag cgccgaatac
     7141 gcgccgagca gcagctgctg ccccgtctgt cctgctgcat agaacgtccg ctgcacctgt
     7201 acaccgccga aagagcggtt gctgagcatc ccgccatatt cgcgtgcgaa gggaacgccc
     7261 tgcgcaacgc actggtcgat gatgttcgcg ctcacttccg caagacggtg aacgttggct
     7321 tcgcgggaac gataatcgcc gcccttgatc gtatcgtaga aaagacggta cgtgctgtcg
     7381 ccgtcgttct ggtaattttt cgctgcgttg atgccgccct gcgcggcgat agagtgtgcg
     7441 cggcgggcgc tgtcctggaa gcagaacact ttcaccttgt agccgagctc agcaaggctg
     7501 gccgcggccg aggcgccggc gagaccggag ccgacgacga taatttccag gctgcgcttg
     7561 tttgccgggt ttaccagcgg aacagtggag cggtacgtcg tccacttctg gtccagcgca
     7621 ccttcaggga tttttgattc gagtttggcc ataactttta tcgtattccg gagagcaaaa
     7681 acgcggcgaa aaaaaacagt ctgcggcgtt cctgtgttcc gtgattttat tttttgttac
     7741 atgttccagt gaagaagatc cttgtgcatg agcttcgcgc cgtagaaaag aatagggaaa
     7801 gacgcgaaag cgattcccat cagcacggca aagcaggtgg agaccatttt ccacgtggat
     7861 gcgtattttt tattgtcgag accgagcgta tgaaaagcgg aggcgagtgc gtggctgagg
     7921 tgaagcgcga gaaagatcac accggcgagg taaagcccgg cgtagagcgg gttgcccagc
     7981 gcttcggcca ctttttcggc aatcgtgagc tgctcatcgc cgtgccccac ggttcccatg
     8041 atgcgatacg gaacgaagaa attatacagg tgaacaacga tgaaaaagaa aaccacgccg
     8101 ccggtgagga gcatgttgcg cgacacccag gggcttgtct gtgaaacgcc gttcaccgca
     8161 tattttaccg ggcgtgcgcg gcggttctcg ccggtgagaa tgagcgcctg cgcgacgtgc
     8221 gcggcgatgg cgaggaagag cacgacctcg atgatgcgga tcaggatatt gcggacggcg
     8281 tcgtgcgaat attcattgaa ggctgcgccg ccgtctccga aataaagcag aacgtttccg
     8341 taaatatgtt ccaggagaaa caggatgagg aacagccccg agagcgccat cactgttttt
     8401 ttgccgatgg aagaggtgag gaatttgttc atatggaagg tgaaattatg cgttttgact
     8461 ggcgggacaa atgtaggtac agcggcggtt tgtatcaagt ttatttgcca tgaaaaatca
     8521 cccgggaacg cgcggaactg ttgaaaagtg cagcttattc gaactgattc taaataaaac
     8581 cgggaaacgc agccccgtca ggcttcttcc ttcaggtcag cgtctttttc tccgaaggga
     8641 ctttctcctt ttcgttcgcg cagatgcagg aacatgcttt tgacgatctg gtctgcgcgt
     8701 tttccgtgcg cacctatttt ttcgaggttg tctttcaatt gtgccaatag ccgtgcgcgt
     8761 tcttcttccg atcccgcgtg cacgatctcg ttcaccagct cggcggatat ttcggaaaaa
     8821 ttattgacga agttgagcgg gttctggatc tcgtgcgcga ttttcgccgt gagcgtgcct
     8881 acggccgctt ttttctctgc gcgcgcgact tcttcggtcc gttcttccag cgctttttcc
     8941 gtttcgaaaa tagcgtagtt gtaaaacccg gcccagatga acaggaaaaa gaacgatagc
     9001 gtgatgttgc tgatgtcgag cacgagcagc acggttttgt cgatcgtata cagcggcgcc
     9061 gatttgcgca gcaccagctc gatgaaaaga aaaccggcca cgcagagcag ggcgaaaata
     9121 tatttgacca acgtgttccc tttcggcatc aggaaaagca gcatggacgc gatgatgaga
     9181 atgtactgga aaccggcgtc ccacccgagc acgtaaacac cgagtacctg gtgcgcgatc
     9241 acttcagaga tgcccatcag cagcgccgtg agatgatgcc cgcgtttatt cagccagatg
     9301 aaaaagacga acagcgcaac gctgaaaaaa ttgaaataga aaagcgggta ggtgtgcgtg
     9361 atgccgaaaa gcacgagcca gctgaaatgc gtgagtccgc ccaccgtgcc ggcaatgtgg
     9421 ttgacgacat agaacctgta gttctttacc ccgatgtccg gcggtgcgac ggtgaagtaa
     9481 ttgcggatgg cgctcataag cggaaaacgg aacgctacaa gttagcagaa cattttaaac
     9541 cgggaatgag gaatggatga accggccaat ttcattggcg gaccgaagaa atgaaaagac
     9601 ggggttaacg gatgaggaag gatgaatgat caaaaattac attcgggatt ttttcgaatg
     9661 ggtttatctt ccttcctgtc attgcgtccc ggcggaaaag gaaaagagat gaagccggaa
     9721 gaattttttt gcggatttgt gagaacagct ttaacgatgt ccggcgaccg gaaaaaattc
     9781 agaatttccc gaacagcttt aagaattcct cccgtttcct gacggaaata tcgatccacg
     9841 catcgttcgc catgagcacg cgcgttccgt cggtgcggga atattttttg atgaagttga
     9901 ggttgatgag ccatgatttg tggcagcgga agaacacctg cgggttgagc atctcctcga
     9961 actctttcag gttgcgcgtg gaaagtattt tttcgccttt gttcgtgacg acgttcgtgt
    10021 agttcatgtc ggcttcgcag tagatgatct cgcccgtgtt tacgaacgaa tagccgtccg
    10081 aactcgggat ggcgatcttg ctgaacgact gcgaatcgtt gccgagattt tccagcagca
    10141 cggaaatttt cttctggctc atcgtcacat gctgctgctt ctcgaatttc tccacggcgc
    10201 ggacgagctc gcggtaatcg accggcttga gcaggtattc cagcgccgac gacttgatgg
    10261 cccgcagcgc gtatttctcg tgcgccgtcg tgaaaatgac ctggaagaac ggatcgggat
    10321 aatgcctgaa aatgtcgaag cccagctctt ttttcagctc tacatcgagg aagatcagct
    10381 cgggcttgtg cgcgtcgatg agccgtatcg catccgccac attctctgcc gtggcaacga
    10441 ccgcaacctg ccggcaatac gcgttcagca gctctttcag cgacccgacg ttcccttttt
    10501 cgtcgtctat gattatcgtt ttgatcaccg ggtaaaaata caaaacgcac aaagcggaaa
    10561 gccgtacgcg gcagtgaaaa acgataatcc tccgaacgct gacgttcatc cgtccatccc
    10621 ggacgttccg taaaaacgca gaacgcgccc ggtcattacc cgcaatttgg cagcataaaa
    10681 atcaacgcca tgaaaaaatt cctgcttgct cttgttgccg cgcttcctgt tgttctcggt
    10741 gcgcagaccg tttcctatcc cgacaccgtg cttgacttca gcacggaata ttttccatcc
    10801 gattccggct gcacggccaa ctggtcggcc tgccgcgcgc tcggtgcgcc ggatgtttat
    10861 cccgcctggg gtgataacag caacgcgtgg acagcgacat ccaccaccgg gaccagttcc
    10921 cccgttccgg gacgcgaatg gatcgtgctc ggctttactg caccggtcac tgcagattcc
    10981 gtcatcgtgt acgaaacctg cagccgcggc ggaaccgaca ccgtttacgt gcgcgatgca
    11041 tccagcggca actggacggt gatctgggac acgaccgcgt acgacggaaa tattccggat
    11101 tcctgcaacg cgatccacat cggcctgtca tcttcgatga acatcgacgc agtgcgcatc
    11161 gccatcgata acgcgcatta tgctttctgg aacgagatcg acgcggtggg cgtcatcagt
    11221 tccggcacga ccggcgttgc agcgggaacg ggcatttcgc ggccggtcat ttttcccaat
    11281 cccggtaacg gcacattcac gctcacgctc ccggcaggat ggaaaagctg ctccatggaa
    11341 attttcgaca tgacaggcag gctgtgcttt gcgcagtacg gcatcgcgtc gtccacattg
    11401 cagatcgaaa caaaactcca gcccggcacc tacccggtaa aacttgcttc gggcgaagaa
    11461 cagcaggtgc agatgatcgt cgtgcaatag tttatcacag gtaaaaccag cgaaatgaaa
    11521 aaagtaattt ttacctcagc aattcttctc gcttcactgg cggcaggcgc ccagggtctc
    11581 gcgaatgtgt ggaacggttt ttacaaccag gtttcaccgg tcatcaccac cgacgtgcaa
    11641 tgctcctggg tggatgctgc gggaaacctg tacaccgcgg ggatgcagca atcggatgtg
    11701 gacgcggaca tcgccaccgc gaatgtcacc acgctgggga attccggctc caattatttc
    11761 tatttctgca aatacaattc ttcaggcgtg ctgcagttgg cgaaacagct gaattcgtct
    11821 tcggtgaata caaccgatat ggccggcgga attgtttcgg atgcgtcggg caatatttac
    11881 gtgacgggac attttacggg aacagtcgat ttcgatcccg gcgcaggaac gtcagctaaa
    11941 acaagcacgt cgggaggttc aggcttcctt gcgaaataca gctcaaccgg tgcgctgctg
    12001 tgggtgcaga atttcggaaa tatttacacg tacccgcagg acattgcgct caatcccgtc
    12061 aatggaaatg tgtggatcgg cggctatctt attgccaaca gctcgatcgg ttatgccgac
    12121 atggatccgg gcgcaggcac ggcgagcaat ttcggaaact cgaatggttc cgtaggtttt
    12181 tactgcgcct acagcgcggc ggcaggaagc tacatcgcga ataccaatta cgcgatctgt
    12241 tccacgacgc tgaactacga ttgcagcgtc aatgcgatct gtttcgacgg aacgacgtcc
    12301 aacatttacc tcaccggttc tttccgcgga accatttatc ctgataacag cacttccggc
    12361 ggaccaccaa ttaccacgag cgccaccaaa gcgatgttca cgatcaggca ggacatcgtc
    12421 aatacgaatg tgaatgcctc gtacgattat ttcgacggat cgccggtgaa cgaaggaacg
    12481 gatatcgcag tggatgcttc cgggaatatt ttcacggcag gctat
//
LOCUS       DASTSF010000377         3431 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_248984,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000377 DASTSF010000000
VERSION     DASTSF010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3431
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_248984"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            19..2247
                     /gene="purL"
                     /locus_tag="VFU15_10815"
     CDS             19..2247
                     /gene="purL"
                     /locus_tag="VFU15_10815"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012926839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HEU4718319.1"
                     /translation="MTTSSTETLATVETAKELGLLPEEFEKIKSILGRTPNFTELSIY
                     SVMWSEHCSYKNSIVWLKTLPKDGPHMLAKAGEENAGLIDIGDGLGCAFKIESHNHPS
                     ALEPYQGAATGVGGINRDIFTMGARPVAQLNSLRFGDPKLEKTKWLVRGVVKGIGDYG
                     NAFGIPTVGGEVFFDDCYNVNPLVNAMSVGIVKAGETVSATSYGAGNPVFIVGSATGK
                     DGIHGAAFASKDITEDSAKDLPAVQVGDPFQEKLLLEASLEVITTGAVIGMQDMGAAG
                     ITCSTSEMSAKGKSGMNVWLDKVPTRQDNMKAWEILLSESQERMLVVVKKGMEDKVQK
                     VFEKWDLNCVQIGEVTDGDRLRYYMNGELVADVPGESLVLGGGAPVYKREWKEPAYFS
                     EAKKFSIDSVKMPYDLKAVAKHLAGHPNIASKQWVYNQYDSMVGTVNMSTNVPSDAAI
                     VNIKGSDKALAMKVDCNSRYVNADPENGCAIAVSEAARNIVCSGGEPSAITNCLNFGN
                     PYNPEVYWQFVGAIKGMGKACTKFATPVTGGNVSFYNQSSYEGPVFPTPTIGMIGVVT
                     HKKNILSLDFKKEGDGIFLIGKSVNDIASSEYLYSFLKVKSSPAPYFDLDEEFNMQAA
                     VKAANYAGLLSSAHDCADGGLFITLLESAMPRNLGCEIASDKNIRKDAFLFGEAQGRV
                     VATVSAANEKAFIAQMEKSGVPFSKLGTVKGKNIVIDGEDFGSVASFRELYANSIGAL
                     MN"
     gene            complement(2441..>3431)
                     /locus_tag="VFU15_10820"
     CDS             complement(2441..>3431)
                     /locus_tag="VFU15_10820"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HEU4718320.1"
                     /translation="SAAVPVSCNGGSDGSAVMNATGGSPPFTFSWSPSGGTGQTANGL
                     TAGIYTVTATDTSGCTTTQTVAITQPTAIVVTTTGDTACAGVNGNISVNSSGGIPPYT
                     YLWSDGSTTPSISVSPVTSTTYTITVTDVNGCAVSGTASVFVGPNPVASFTAPAVVNG
                     VFNLSGDPSQLCFTDGSSNAASWYWNFNSQDSAYTQDPCVNVGTSDKGTYCVTLVVHR
                     AIGCVDTTQECIEINDVNYSIPNVFTPNGDGSNDAFIITNSGMASLHCEIYDRWGALI
                     YEWNDPAGYWDGKTNNGKEAVDGVYYYVLYMADYTGKNYNETGFVHLIRGTQK"
BASE COUNT          790 a          914 c          955 g          772 t
ORIGIN      
        1 tctcaaaaac acttcctgat gaccacgtcc tccacggaaa cccttgccac cgttgaaact
       61 gcgaaagagc tcgggctcct tcccgaagaa tttgaaaaaa taaaaagcat cctcggccgc
      121 acgccgaatt tcaccgagct cagcatttat tccgtaatgt ggtcggagca ctgctcgtat
      181 aaaaattcca tcgtgtggct gaaaacgctt cccaaggatg gtccgcacat gctcgcgaaa
      241 gcaggcgaag aaaacgccgg cctcatcgac atcggtgacg gtctcggctg cgcgttcaag
      301 atcgaatcgc acaaccatcc ttctgcgctc gaaccgtacc agggcgctgc cacgggtgtc
      361 ggcggcatca accgcgatat tttcaccatg ggcgcgcgtc ctgtggcaca gctgaattca
      421 ctgcgtttcg gcgacccgaa gctggaaaaa acaaaatggc tcgtgcgcgg tgttgtgaaa
      481 ggcatcggtg attacggcaa cgcgttcggc attcccactg tcggcggtga agtttttttc
      541 gacgactgtt acaatgtcaa cccgctcgtg aatgcgatgt cggtcggtat cgtgaaagca
      601 ggtgaaacgg tttccgctac gtcttacggt gcgggaaatc ctgttttcat cgttggttcc
      661 gcaaccggaa aggacggcat tcacggcgct gcgttcgcat cgaaagatat tacggaagat
      721 tccgcgaaag atcttcccgc tgtgcaggtg ggcgatccgt tccaggaaaa attattgctc
      781 gaagcttccc tcgaagtgat cacgacaggc gcggtgatcg gtatgcagga catgggcgct
      841 gccgggatca catgttccac gtcggaaatg tccgccaaag gaaaaagcgg catgaacgtg
      901 tggctcgata aagttcccac gcggcaggac aatatgaaag cctgggaaat ccttctttcc
      961 gaatcgcagg aacgcatgct tgtggtcgtg aagaaaggaa tggaagataa agtgcagaaa
     1021 gtgtttgaga aatgggacct caactgcgtg cagatcggtg aagtgaccga tggtgaccgt
     1081 ctcaggtatt acatgaacgg cgaacttgtt gcagacgtcc ccggtgaatc gctcgtgctc
     1141 ggcggcggcg ctcctgttta caaacgtgaa tggaaagagc ccgcgtattt ttccgaagcg
     1201 aagaaatttt ccatcgacag cgtgaagatg ccttacgatc tgaaagccgt cgcgaaacat
     1261 cttgcaggac atcccaacat cgcgtcgaag caatgggtgt acaatcaata cgattccatg
     1321 gtgggaacgg tgaacatgag cacgaatgtt ccttccgatg cggccatcgt caacatcaaa
     1381 ggttccgaca aagcgcttgc gatgaaagtg gattgcaatt cgcgttacgt gaatgcggat
     1441 cccgagaatg gctgcgcgat cgcggtttcc gaagcggcgc gcaacatcgt ctgttccggc
     1501 ggcgagcctt ccgcgatcac gaactgcctc aatttcggga acccttacaa cccggaagtg
     1561 tactggcaat tcgtcggagc gataaaaggg atgggaaaag catgcacgaa attcgcgacg
     1621 ccggtcaccg gtggaaacgt cagcttctac aaccagtctt cgtatgaagg ccccgtgttt
     1681 cccacgccga ccatcggcat gatcggtgtc gtcacgcaca agaaaaacat cctttcgctg
     1741 gatttcaaaa aagaaggcga cggcattttc ctcatcggga aatcggtgaa tgatattgct
     1801 tcctccgaat acctgtattc gttcctgaaa gtgaaaagtt ctcccgcacc gtatttcgat
     1861 ctcgacgaag agttcaacat gcaggcagcg gtgaaagcgg caaattacgc cgggctgctc
     1921 agttccgcgc acgactgcgc cgacggcgga cttttcatta cgctcctgga aagcgcgatg
     1981 ccgcgtaatc tcggctgtga aattgcttcg gacaaaaaca tccgcaaaga tgcgttcctg
     2041 ttcggcgaag cgcagggccg ggtagtggcg actgtttccg cagcgaacga aaaagcgttc
     2101 atcgcccaaa tggaaaaatc tggcgttcct ttttcgaagc tgggaacggt gaaaggaaaa
     2161 aatatcgtga tcgacggcga ggatttcgga agcgttgctt cgttcaggga gctctacgcg
     2221 aattcgatcg gcgcgctgat gaattgatcc tgctcccggg aatcttcagc cataaataac
     2281 acgaagtttt ttcacgaagt acacgctgtg atcttaacac ggagaaccgt taagatcgtt
     2341 tcacgcccag gttttaaaag ttccgcgttc cgggtttcga actcagaaca gcaaaccggg
     2401 aactaaaaac cttcaaagct ccaaagctgt ttcccggatt ttatttctgt gtcccgcgga
     2461 tgaggtgcac gaaccctgtc tcgttgtaat tcttcccggt gtaatcggcc atgtacagga
     2521 cgtaataata aacgccgtcc accgcttctt tcccgttgtt cgtttttccg tcccagtaac
     2581 ctgcgggatc gttccattcg taaatgagcg cgccccagcg gtcgtagatc tcgcagtgca
     2641 gcgacgccat tcctgaattc gtgatgatga acgcatcgtt cgatccgtcg ccgttcggtg
     2701 tgaacacatt ggggatggaa taattcacgt cgttgatctc gatgcattcc tgcgtcgtat
     2761 ccacgcagcc gatcgcacgg tgcacgacga gcgttacgca gtaggttcct ttatcggatg
     2821 tgccgacgtt cacacagggg tcctgcgtat aggcagaatc ctggctgttg aagttccagt
     2881 accaggaagc cgcgttcgac gatccgtcgg tgaagcagag ctggctcgga tcgccgctga
     2941 ggttgaacac gccgttgacc acggccggag cggtgaatga cgcgacggga ttcggtccga
     3001 caaagaccga cgctgttccg ctgacggcgc acccgttcac atccgtcacc gtgatcgtgt
     3061 acgttgtgct ggttaccggt gaaacactga tggacggtgt tgtcgatccg tccgaccaca
     3121 ggtacgtgta cggcgggatt cccccgctgc tgtttacgct gatgttgccg ttcactcctg
     3181 cgcaggcggt atcgccggtg gtggtgacga cgatcgcagt gggttgtgtg atcgccacgg
     3241 tctgtgtcgt tgtgcagccg ctggtgtcgg ttgccgttac ggtgtaaatg ccggctgtca
     3301 gcccgttcgc ggtttgcccc gttccacccg atggcgacca gctgaaagta aacggcggtg
     3361 aacctccggt tgcgttcatc acggcggacc cgtcagatcc gccattgcag ctcaccggta
     3421 cggccgcgct c
//
LOCUS       DASTSF010000378         6593 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_423144,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000378 DASTSF010000000
VERSION     DASTSF010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 6593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6593
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_423144"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            390..1619
                     /locus_tag="VFU15_10825"
     CDS             390..1619
                     /locus_tag="VFU15_10825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718321.1"
                     /translation="MKKIIPVFLLFACLEGYAQDQSQHIADSLRKADSIANAVAQQNV
                     KPPVLPAPVINTMGVEKFEMPPNGTPGATSGLTANKIFPGDFIGIKMDTNGCRLAEQN
                     LDTFRLWINGLCYTKIKPAFLDRNKSTLIFRLDYDTSASSPWKLFYALPNYWTFRHQV
                     VMNAGTLTKEFRSAGKGPQPPNADLFTSAPWMLITGYSIFGALLFVVFFFGKGIIKDV
                     GLYAQNGVKVTYKKNEPTDIATGVINVRDLPYSLSRFQFLFWLIIIFFGITHIWAITD
                     TLTTPTGTVLLLLGISGGTFYISRLIDANPSDAAPAPGANTDPATAEAKLSSDLVQKF
                     VDNKQKSKGFLFDMLNDGKGISLHRLQLVVFTIFLGTFFVWQVLYNLGLPEFSATMMT
                     LMGISSGTYAGMKTTEK"
     gene            1648..2070
                     /locus_tag="VFU15_10830"
     CDS             1648..2070
                     /locus_tag="VFU15_10830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718322.1"
                     /translation="MTDLEINQSAPAVVPAKDPWYATGIFLRREGIYTLEVTPPGQTW
                     KDGDGLGDISADGKRALQLIGLYPFIRMPFEKWFALLGCIGRKRSTYFRIGSQRKDYS
                     PPEDGELTCFANDALGFNDYWYSHNNEGEMHITVTRTA"
     gene            complement(2143..3045)
                     /locus_tag="VFU15_10835"
     CDS             complement(2143..3045)
                     /locus_tag="VFU15_10835"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEU4718323.1"
                     /translation="MKKLYTLALLACTSVAVSSQNLVPNPGFETQDTCPSVSEIWKAP
                     PWVSATLGTPDLYNSTCSSQNFPGRTGIGSSGVYAMNTFSNNREYMIAPLTSPLAGGT
                     TYYVSFWVKRANYRYAVDQFGAYFSTSNINQTTTGVLNYTPQVHNLTGNFLSSSTSWM
                     QISGSFVAAGGESYIVIGNFSNDANTDTLVANSSSSSKVAYYMVDDISVNASGVGIDE
                     NLVAGGIIVYPTPASDRINVQADGAGSVTLLDATGRLVQNMQVENPQPGKFVLNVAEC
                     SEGVYFLNIYSDNGVTTKKVVITR"
     gene            complement(3217..3669)
                     /locus_tag="VFU15_10840"
     CDS             complement(3217..3669)
                     /locus_tag="VFU15_10840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEU4718324.1"
                     /translation="MKTTINMKKKDFHTTITVTASPEEALEKISRVNGWWAKNFQGKA
                     GKLNDRFSVHFGKTFVDFEVSELVPGKKVVWKVTDCNLHWINNRKEWKGTEVVFEVSG
                     KKNATQIDFTHIGLVPGVECYTDCEAGWTGHVTGSLVKFINEGKGMPE"
     gene            complement(3681..4127)
                     /locus_tag="VFU15_10845"
     CDS             complement(3681..4127)
                     /locus_tag="VFU15_10845"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEU4718325.1"
                     /translation="MEKKNYHRTISVSASPEATMKKISQVNRWWKKDFSGSAGKVNDR
                     FTVPFGELSGESSFVDFMVTEMTADKKTVWKVTDCNLPWFSNKKEWKATEVVFELSEE
                     NGKTRIDFTHVGLVPEVECYEACEKGWDSHVTRDLLAFIDEEKTIS"
     gene            complement(4151..4477)
                     /locus_tag="VFU15_10850"
     CDS             complement(4151..4477)
                     /locus_tag="VFU15_10850"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEU4718326.1"
                     /translation="MKAQTLDAFQVIADPSRRRMLQLLSKNTMTINSLAENFDMSRPA
                     VSKHIKIMYNAGFISIKDIGRERYCTLKQDGFNELQDFIDYFDKFWTTKLGKLQALLN
                     NKSKKQ"
     gene            complement(4477..4902)
                     /locus_tag="VFU15_10855"
     CDS             complement(4477..4902)
                     /locus_tag="VFU15_10855"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEU4718327.1"
                     /translation="MEKEIRQQWHFDQAPEEVWEYLTKPELIEQWLMKTDFKPVVGQK
                     FQFTFTAKPGSKYDGVVNCEVLEIIPYSRLSYSWDGHLQDKSRYFNSVVVWTLIPKNN
                     GTELQLEHNGFEMLEDILNHTNGWKSCLQKMADHINTVK"
     gene            complement(5244..5315)
                     /locus_tag="VFU15_10860"
     tRNA            complement(5244..5315)
                     /locus_tag="VFU15_10860"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5281..5283),aa:Met,seq:cat)
     gene            complement(5625..>6593)
                     /locus_tag="VFU15_10865"
     CDS             complement(5625..>6593)
                     /locus_tag="VFU15_10865"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEU4718328.1"
                     /translation="TVTVNVYTPPAVIATASVDSFCSGTSTTLNAGGAASYSWSPGNM
                     TGANITVSPSSSTNYIVTGTDTNGCTNADTVMLTVYPSPTLTVTSTNSVICTGGSTSL
                     MASGATTYTWQPGNLTGATVTVTPATSTNYTVTGVDTNGCTGISVYGMSVAAPPNVYG
                     YSSLSNACTNDPPILLTGIPAGGTWSGPGVAGNNFSPSVAGAGTHTLTYSYTDTYGCS
                     NTATVTIVVDPCAGVHEQSALNGVSFYPNPNAGSFTLNVQSGDIHTLTMEIFDLQGRI
                     VYSQQLDGISSGYSKDVDVSFLANGAYYLRFTSDSSSQTTRLIIEK"
BASE COUNT         1511 a         1691 c         1668 g         1723 t
ORIGIN      
        1 tcgaccggaa gattgtcccg gggtctgctt tttcgttttg tgccacgggt gcaggaggat
       61 agttttagga gcacttcaaa agtatgaata atcgatgcgc gcatcttttc tgatcgacag
      121 gtggtcagca atctccgatg aaaggaaaca tgcatcccgg aaccggatcc cataagcata
      181 ccggttgttc tttggagata ataaatccgg aaatccattg catgccccgt atgtaaaaag
      241 tcccggttgt ttttaaattg gcgtatataa ttgtcatttt taatttgcaa accggataat
      301 tagcctacat tagaaatgtg ccggaatttt tcacccccgg cttttagggt cagttcccgt
      361 tccgaaaaaa caacgcaatc tccgttcaga tgaaaaagat cattcccgta ttcctgctgt
      421 tcgcgtgcct ggaaggttat gcgcaggacc agtcgcagca catcgccgac agcctgcgaa
      481 aagcagattc catcgccaat gcggtcgcgc agcaaaatgt gaaaccgccc gttctcccgg
      541 cgcctgtgat caatacgatg ggcgtcgaaa aattcgaaat gcccccgaat ggtacacccg
      601 gcgcgacgtc cggtctcacc gcgaacaaaa tctttcccgg cgattttatc gggattaaaa
      661 tggacacgaa cgggtgcagg ctggccgagc agaacctcga cacattccgg ttgtggataa
      721 atggtctctg ctacaccaaa ataaaaccgg catttctcga ccggaacaaa tccacgctta
      781 ttttccggct cgattacgac acctccgcgt catcgccgtg gaagttgttc tacgcgcttc
      841 cgaattactg gacgttccgc caccaggttg tcatgaacgc cggcacgctc acgaaggaat
      901 tcaggagcgc aggcaaaggt ccgcaaccgc ccaatgccga ccttttcacg agcgcgccgt
      961 ggatgctgat caccggctat tccattttcg gtgcgttgct tttcgtggtt ttctttttcg
     1021 gaaaaggcat catcaaggac gtaggactgt atgcgcagaa cggcgtgaag gtcacgtaca
     1081 aaaaaaatga gccgacggat atcgccacgg gcgtgatcaa tgtaagggat ctgccgtaca
     1141 gtctttcgcg cttccagttc ctgttctggc tgatcatcat ctttttcggc atcacgcaca
     1201 tctgggcgat caccgatacg ctgaccacgc cgacgggaac tgttctgctg cttctcggca
     1261 tcagcggcgg gacattttac atcagccgtc tcatcgatgc gaatccttcg gacgcggcac
     1321 ccgcacccgg tgcgaataca gatcccgcca ctgcggaagc gaagctttcc agcgacctcg
     1381 tgcagaaatt cgtggacaac aagcaaaagt cgaaaggctt tttgttcgat atgctcaacg
     1441 acggcaaagg aataagcctg caccgcctgc agctggtcgt cttcaccatt ttcctcggta
     1501 cgtttttcgt ttggcaggtg ctgtacaacc tcggtctgcc ggagttcagc gcgacgatga
     1561 tgacgctgat gggcatcagc agcggaacct acgccggaat gaaaacaact gaaaaataaa
     1621 aactgaaaag ttcattccac ctttgtaatg accgacctcg aaataaatca atccgcaccg
     1681 gccgtcgttc cggcgaaaga cccctggtac gcaaccggaa tattcctgcg cagggaagga
     1741 atttatacgc tggaagtaac gcctcccggg caaacgtgga aagacggaga tggtctcggt
     1801 gatatttccg ccgacggaaa acgggccctg cagcttatcg ggctttaccc gttcatccgg
     1861 atgccatttg aaaaatggtt cgcgcttctc ggctgcatcg gcaggaaacg ttcgacgtat
     1921 ttccggatcg ggtcgcagcg gaaagattat tcgcccccgg aagacggtga gctgacgtgc
     1981 ttcgcaaacg acgcgctcgg cttcaatgat tactggtaca gccacaataa cgaaggcgaa
     2041 atgcatatta ccgttacgcg gacagcgtaa gagggccttc agaaaacagg tatgaaaaaa
     2101 gccggctgaa tgccggcttt tcgtttatca cagaaacgag tgttatcgtg tgatcacgac
     2161 ttttttcgtc gtcactccgt tgtccgaata aatgttgagg aagtaaacgc cctcgctgca
     2221 ttctgcgaca ttgagcacga attttccggg ctgcggattt tcaacctgca tgttctgcac
     2281 aagacgcccg gtggcgtcga gcagcgtgac cgatcctgcg ccgtccgcct gcacgttgat
     2341 gcgatcggaa gccggtgtag gataaacgat gatgccgccg gcgacaagat tttcatcgat
     2401 gcctacgccg gatgcattta cagaaatgtc gtcgaccatg taatacgcca ctttgctcga
     2461 gctggaactg ttggcgacca gcgtgtccgt gttcgcatcg ttgctgaagt tgccgatcac
     2521 gatgtagctt tctccgcccg ccgcaacaaa cgatcctgat atctgcatcc acgatgtgct
     2581 cgagctgaga aaattcccgg tcaaattatg cacctgcggc gtataattca gcacgccggt
     2641 tgtcgtctgg ttgatgttgc tcgttgaaaa ataagcgccg aactggtcga ccgcgtagcg
     2701 gtaattggcg cgcttcaccc agaaagaaac ataatacgtg gtgccaccgg cgagcgggga
     2761 agtaagcggc gcgatcatgt attcgcggtt attcgaaaac gtgttcatgg cgtacacacc
     2821 cgagctgccg ataccggttc ttccggggaa attctgcgag ctgcaggtgc tgttgtaaag
     2881 atcgggtgtg ccgagcgtgg ccgacaccca cgggggcgct ttccatatct cgctgacgga
     2941 agggcacgtg tcctgggttt cgaaacccgg gttcggcaca aggttctggc tgctgacagc
     3001 aacagaagtg catgccagca acgcaagcgt gtaaagcttt ttcattggag ttgttttttg
     3061 gggtttataa atgaggttaa caggaatgga aagttacgca aataaaaaac ggcattcctt
     3121 attcaccgca gcaacggttt ccccgtgatg aaaaaaaccg cgttgtatga acgcggtctt
     3181 tgccgacatg aagcgctcat gccgctcaac tgttatttat tccggcatcc cttttccttc
     3241 attgatgaat ttcaccaggc tgccggtaac gtgtcccgtc catcctgctt cacaatccgt
     3301 gtaacattca acgccgggca ccaggccgat gtgggtaaaa tcgatttgtg tggcgttttt
     3361 ctttcccgaa acttcgaaga caacttcagt gcccttccat tctttccggt tattgatcca
     3421 gtgcaggttg cagtcggtta ctttccacac cactttttta cccggcacta attcactgac
     3481 ctcgaaatcg acgaacgttt taccgaagtg aacgctgaac ctgtcattca gctttcccgc
     3541 tttaccctgg aagttctttg cccaccagcc gttcacccgg ctgatctttt ccagcgcttc
     3601 ttcgggcgaa gcagtaacgg ttattgtcgt gtgaaaatct ttctttttca tgttgatcgt
     3661 tgttttcatc tgaacgtgtt ttagctgatt gtcttttctt catcaatgaa tgccagcagg
     3721 tcccttgtta catgactgtc ccatcctttt tcacaggcct cgtagcattc gacttccggc
     3781 accaggccga catgcgtaaa atcaatcctg gtttttccgt tttcttccga aagctcaaaa
     3841 acaacttcgg tggctttcca ttcttttttg ttgctgaacc agggcaggtt gcagtccgta
     3901 actttccaga ccgttttttt gtctgcggtc atctcggtga ccatgaaatc gacaaaagaa
     3961 gattcgccgg aaagctctcc gaagggaacg gtgaatctgt cattcacttt tcccgcgctt
     4021 cccgaaaagt cctttttcca ccagcgattt acctggctga tttttttcat cgttgcttcg
     4081 ggggaagcgc tgacagaaat tgtcctgtga taatttttct tttccatgtg gttgagtttt
     4141 agattgtgtg ttattgcttt tttgatttgt tgttcaggag cgcctgtaat tttccgagtt
     4201 tcgtcgtcca gaatttgtcg aaatagtcga tgaaatcctg cagctcgttg aagccgtcct
     4261 gcttcagtgt gcagtagcgt tccctgccga tgtctttgat ggaaatgaac cccgcgttgt
     4321 acatgatctt gatgtgtttc gaaacggcgg gacggctcat gtcgaaattt tccgccaggg
     4381 aatttatcgt catagtgttt ttggaaagca gttgcagcat tcgcctcctg ctcgggtcgg
     4441 cgatcacctg gaaggcgtct aacgtttggg ctttcattat ttgacagtat taatatggtc
     4501 ggccattttt tgcaggcagc ttttccaccc gttcgtgtga ttgagaatat cttccagcat
     4561 ctcaaatcca ttatgctcca attgcagttc cgtaccgttg tttttgggaa tgagcgtcca
     4621 tacaacaacc gagttgaagt acctgctttt atcctggaga tgtccgtccc atgaatagga
     4681 taatctggaa taaggtatta tttccaatac ttcgcagttg accactccat cgtatttact
     4741 gccgggttta gcggtaaacg tgaactggaa tttttgccca actaccggct taaaatccgt
     4801 tttcatgagc cattgctcaa tgagctccgg cttcgtcagg tattcccaca cttcttccgg
     4861 tgcctggtcg aagtgccatt gttgtctgat ctctttttcc atgataagta acttttgagt
     4921 tacccaaaaa taggtaactt ttgggttacc ttacaaattc cggcaaaatt tttttcaacc
     4981 ggcaggcaaa cggggcctgg aaagcgcggc agcggcgtgg gcaggatgct gatctcgtcg
     5041 caaattcttc cgtataaatc aaaatccagg ggcgatgctt ggaagtaaaa gtcctcagcc
     5101 cggtttcatt gtgaaagact aaacgttctg tgagatcacg tgtttgaccc acatacgttt
     5161 tctctgcgga aacggaataa agaatgtaaa ccgtgaatgc cataaaaaag aaagagtctc
     5221 ccataattga gagactctct ttgtagcggg aacaggactc gaacctgtga ccttcgggtt
     5281 atgagcccga cgagctacca ctgctccatc ccgcatcata ttttcactgc acacgctccg
     5341 ggttattgtc ccggtgagct atccgccttc ggcggactcc atcccgcatc atattttcac
     5401 tgcacaccct ccgggttatt gtcccgacga gctatccgcc ttcggcggac tccatcctgc
     5461 atcattccgg tgataaccgg tattgctttc ttaaaagcgg tgcaaatata gggttatttt
     5521 cggtcgtcgc ccagattgtt atcaacagcc tgctttttcg gctttccgta aaaagaaaat
     5581 gcccatgaat ctcatgagca ttttccggtg caaaacggtg tgctttattt ctcgatgatc
     5641 agtcgcgtgg tttgcgatga gctgtcggag gtgaagcgca ggtagtaggc gccatttgca
     5701 aggaacgaca cgtcgacgtc tttcgaataa ccggatgaaa ttccatccag ctgctgcgag
     5761 taaacaatgc gaccctgcag atcgaagatc tccatcgtca gcgtgtggat gtcgcccgac
     5821 tgaacgttca gcgtgaatga accggcgttc gggttcgggt agaacgacac gccgttcagc
     5881 gcgctctgct catgaacacc ggcgcaggga tccaccacga tggtgaccgt tgctgtattc
     5941 gagcagccgt aggtgtccgt gtaagaatac gtcagcgtgt gtgtgcccgc accggcgacc
     6001 gacggagaga aattatttcc ggcaacaccc ggacctgacc aggttccacc ggcaggaatt
     6061 cccgtcagta agatcggcgg atcattcgtg caggcattcg agagtgaaga atagccgtac
     6121 acgttcggcg gggcagccac cgacatgccg taaacagata ttcccgtaca gccgttcgtg
     6181 tcaacaccgg tcaccgtgta gttggtcgat gtagccggag taaccgtcac ggtagcgcct
     6241 gtgaggttgc ccggctgcca ggtataggtc gttgctccgg aagccatgag tgatgtgctt
     6301 ccgccggtgc atattaccga gttggtcgat gttacggtga gcgtcgggga aggataaacc
     6361 gtgagcatga ctgtgtcggc attggtgcat ccgttcgtgt ctgttccggt tacgatgtaa
     6421 ttcgtgctgg atgatggtga cacggtgatg ttcgccccgg tcatatttcc aggcgaccac
     6481 gaatagcttg ccgcgccgcc cgcattcagg gtggtgcttg ttccgctgca gaatgaatcg
     6541 acagatgctg tagcaattac cgcaggcggt gtatagacat tgaccgtgac cgt
//
LOCUS       DASTSF010000379         7328 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_484229,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000379 DASTSF010000000
VERSION     DASTSF010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 7328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7328
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_484229"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1472
                     /locus_tag="VFU15_10870"
     CDS             <1..1472
                     /locus_tag="VFU15_10870"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEU4718329.1"
                     /translation="DRIKGTLEEIPNKMVCDAANNTYIIGRFSGMVDFDPDPVATHTL
                     TASGSGHDIFILKLDINGHFVWVSRLGGSSDDDGIGISIDASGNLLVCGYFQGSMSVL
                     IGGGKYTISSSGLEDAFFAKYNTSGSCLGIWRIGGSGTDEATSVKPGAGGNIIVGGEY
                     GSLNCDFDPGTGTVHPASFGGMTDEFVASYSGTASFGWVKCGGDSDNDETSEVVTDLS
                     GNIYACGVAGVGSIDLGGGSMTTAGAPGSGTSDIFLACYNSGGTHQWSELIGSDSDDV
                     PANMVRDDLGYLYITGYFSHIIDFDPGPGILTKTSAGRLDIFIARFGTDGSLRNVDAF
                     GDVHDDGGHGIAVSAELKDIYTTGDFDGTVNFDPFMMAPPLTSVGLTGFGDAFLTRHN
                     WDSLPARLASPGIVSQAPAFSVSAYPNPCSGSLVIRNAAAGSVIELYSADGKKTGSWP
                     VENEGTFSIGLNAYENGIYFLRIVRPGGAVQNEKIVLQH"
     gene            1551..2291
                     /locus_tag="VFU15_10875"
     CDS             1551..2291
                     /locus_tag="VFU15_10875"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HEU4718330.1"
                     /translation="MSAHLILDFGNTTAKAYVFSEGENVSSTVLPFISPAEILSFLSG
                     KKIRAAIIASVTDHPATLEKELADFFPVLTLDHSTKLPVNNLYKTSGTLGYDRLAAAI
                     GAKQLFPASPVLAVVAGTCITYNIIDAENNFTGGAISPGLHMRLRAMHEFTAHLPLAE
                     ITGEIPLTGTDTNSSLLSGAVHGATEEIRGMMHAYTAAFPGLKTVIAGGDGAFLAEGL
                     KNGIFARPDLVAEGLNCILEYHVANHLI"
     gene            2296..3831
                     /locus_tag="VFU15_10880"
     CDS             2296..3831
                     /locus_tag="VFU15_10880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718331.1"
                     /translation="MRKLILTAAIFFSVFATSRAQNPTILTNVSPYSRYGIGDIQFNQ
                     GMFNAGMGGGGIGYRNDSLIPQYINLQNPASYTGHQIVAYEVSLLSNTVQLQNSTTTG
                     TFNRTTLGNFSMAFPVTKWWGAGFGLTPYSNVGYNLSLSDNPANIGPVTYKYEGSGGV
                     DQVFITNAFRPFAGAPRKFLLSDSYDKLKLANDTSAMQRKLKMRNALANISVGVTSSY
                     LFGTLNYVRRDEFPDSLYNFNTKISKRTQFRDFYLNYGVQYTFRLPKSLNPLYHSLPD
                     SVITGKKIFSNYFSYTLHGKSDTAKLFIRKPGIRVSVGFVFSLPTDINVSSDILAQTY
                     KLYGTIEQFRDTVFNNTNIPSRIVIPAMYGAGFALKKDFRWVFQADYMTQLWSQSTIM
                     GQDPGLKDSRRVTAGFQFQPKQAGRGNFLGTGQYRVGVRYYQTYLDLMDTRLTEMSAN
                     LSLAFQVPHNTHIGEPVSRLQVTFEYGMRGTTSNNLVQENFYRVTLGLTINDRWFNHY
                     KYD"
     gene            3844..4407
                     /gene="lptC"
                     /locus_tag="VFU15_10885"
     CDS             3844..4407
                     /gene="lptC"
                     /locus_tag="VFU15_10885"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HEU4718332.1"
                     /translation="MSLSKTYLLFAFAGCFLLHACANDPAKVKAFTRDDRQPVQTAHD
                     VDMLYTDSAKLKIHITSPQVKDFAGSKPYTEMPEGVKVEFFDDAQTVNSYLTSNYAIR
                     KQREQQTEARNDVVVVNIKGEKLNTEKLIWDEAKHRIYTDAFVKITTKDQVLMGNGLD
                     SDESFTNYEIKDITGSFTLKDPDKKQP"
     gene            4404..4625
                     /locus_tag="VFU15_10890"
     CDS             4404..4625
                     /locus_tag="VFU15_10890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718333.1"
                     /translation="MKKYLRLTEYSWLFLAIICIGITTYFFIIHDTDSGSIGLMLTLF
                     AGIMYNMRRMFNRRMERMAKKQEEEQQKQ"
     gene            4774..6945
                     /locus_tag="VFU15_10895"
     CDS             4774..6945
                     /locus_tag="VFU15_10895"
                     /inference="COORDINATES: protein motif:HMM:NF025009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HEU4718334.1"
                     /translation="MSRIRSRIGLLVGIIFLALLAFVLTDLFNSQRGIFGGQSSGAVG
                     EINGHSISLGEFRTRLDEASNGQQLNDQQQQQLSEAVWQELLDQYIYKPQYDDLGIAI
                     SDDELTEQMTSDHPSAYMQQFFQDRQSGQIMQQFQGPDGQLSGKAIRDFVAKMPAETE
                     QQWVQIEKDMRKTLLREKYNNLLRKGFYVTTAEAKHEYADENTKYDFNFIVKKYSEIP
                     DSTVKVTDDEMMDYYNQHQYKFKQTDNMRSMEYLAFDVYPSADDIADQRKDMENMVTE
                     FQKQKTAADDSMFAVSMNESGTFDKKYLRPGQFPVGSDSAFLKAKNGDVLGPFNMGGN
                     LDIYKVVGQDNSPDSAKVRHILIAYKGADRAGPEITRTKEQAKMRADSILKVVKSGRT
                     KLEDLVWKLTDDPGSKDQNAKPDAPGYKGDYGWFTHESGFVQEFKDAGFNNPVGSTIV
                     VETSFGYHVIQVLDRTKPTTKVQVIAIEKKVEPSEKTIRIVYNKASEFAGKNNTGDLF
                     TAAVQKNSMQVLKADQIQENSRYISGIENARDIVRWMYDDKTEVGSVSQPFQSGDRYV
                     VCHLTKIINKGVKPFDEVKDICQLEARKIKKGQMFTDQLNKAKGATLDAWAKNVGTTV
                     MPAVNISFAAPYIQAAGYEGTVVGVASTLKPNTLSAPIKGSMGVYVVSLTKVTKADPL
                     LDIAGEKAKVLQNNYSGRADGVANEVLKDDANVVDNRAKHF"
     gene            complement(7152..>7328)
                     /locus_tag="VFU15_10900"
     CDS             complement(7152..>7328)
                     /locus_tag="VFU15_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010663404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEU4718335.1"
                     /translation="AAIFFIHLIVNTGMTIGLIPVIGIPLPFFSYGGSSLIGFTILLF
                     IFIKLDSQRLLVLR"
BASE COUNT         1822 a         2042 c         1885 g         1579 t
ORIGIN      
        1 ccgacaggat caagggaacg ctggaagaaa ttcccaacaa gatggtatgc gacgccgcga
       61 acaacacgta catcatcggg cgtttttcag gcatggtgga tttcgatccg gatcctgtcg
      121 ctacgcatac gctgacggca agcggaagcg ggcatgacat attcatactc aagctcgata
      181 tcaatggaca ttttgtgtgg gtcagccgcc tgggaggatc ttccgatgac gacggtatcg
      241 gtatatcgat cgacgcatcc ggcaacctgc tggtgtgcgg ttatttccag ggttcaatgt
      301 ccgttctgat cggcggcgga aagtacacga tctccagctc gggacttgag gacgcttttt
      361 ttgcaaaata caatacgtcc ggaagctgtc tcggcatctg gcggatcggc ggctccggaa
      421 cagatgaagc cacttccgtc aagccgggtg cgggcggtaa tatcattgtc ggcggtgaat
      481 acggatcgct gaattgtgat ttcgacccgg gaaccggcac tgttcatccc gcatctttcg
      541 ggggcatgac ggatgaattc gtagcgtcct attcgggaac agcgtcgttc ggatgggtga
      601 aatgcggagg tgacagcgac aacgatgaaa caagcgaagt tgtcacagac ctgtccggga
      661 acatttatgc ctgcggtgtg gccggtgtcg gatccatcga tctcggcggc gggtccatga
      721 ccacagccgg ggcgcccgga tcgggtacgt cggatatttt cctcgcctgc tataattcgg
      781 gaggaacgca tcaatggtcg gagctcatcg gttccgacag cgatgacgtt ccggcaaaca
      841 tggtccgcga cgatctcggt tacctgtaca tcaccggcta cttttcgcac atcatcgatt
      901 tcgacccggg gccgggcatt ctcacgaaaa caagcgccgg caggctcgac atttttatcg
      961 ccaggttcgg gacggacggc tctctccgca atgtggatgc tttcggagat gtccacgacg
     1021 atggcggtca tggaattgca gtcagcgccg aactgaaaga catttacaca acgggcgatt
     1081 tcgacggaac tgtcaacttt gatcccttta tgatggctcc accgctgacg agcgtaggtc
     1141 tcacgggatt cggtgatgcc ttccttacgc gccacaactg ggattctctt ccagcccgac
     1201 ttgccagccc cggtattgtt tcgcaagctc ctgctttctc cgtttccgct tatccgaatc
     1261 cctgcagcgg ttctcttgtc atccgcaacg ccgccgcggg aagcgtcatc gagctgtatt
     1321 cggcagacgg taaaaaaacg ggaagctggc cggtcgaaaa tgaaggtacg ttctccatcg
     1381 ggctcaacgc ttatgaaaac gggatctact tccttcgcat tgtccggccc ggtggtgcag
     1441 tgcaaaacga aaagatcgtt ctgcagcatt aaaaccgcga cagcgcaacc cctgcattca
     1501 tgcggcgatc cgaaagggcc gccgcttttt tgtttgctac ctttgcggcg atgtctgcgc
     1561 atctcattct cgatttcggc aacaccaccg ccaaagcgta tgttttcagc gaaggagaaa
     1621 acgtttcttc gacggtgctt ccgttcatct cgccggcgga aatactttcc tttctctccg
     1681 gtaaaaagat ccgcgcggcg atcatcgctt ccgtcaccga tcacccggcc acgctcgaaa
     1741 aagagctggc tgattttttt cccgtgctga cgctcgatca ttccacgaaa cttcccgtta
     1801 acaatcttta caaaacttcc ggaacgctcg gctatgatcg cctggcggcc gccatcggcg
     1861 caaagcaact ttttcctgct tcccccgtct tggctgtggt ggccggaacc tgcatcacat
     1921 acaacatcat cgacgccgaa aataatttca ccggtggcgc catttcaccc ggcctgcaca
     1981 tgcgcctgcg tgcaatgcat gaattcaccg cgcacctgcc cctggcggaa atcaccggag
     2041 aaattccgct cacgggaacg gacacgaaca gctcgcttct atccggcgcc gtccatggcg
     2101 caacggaaga gatacgcggg atgatgcacg cttacacggc ggcatttccc ggactgaaaa
     2161 cggttatcgc cggtggcgac ggagcgtttt tggcggaagg acttaaaaat ggcatctttg
     2221 cacgcccgga tctcgtcgct gaagggctga actgcattct tgaatatcat gtcgcaaacc
     2281 accttatctg aacgcatgcg gaaattaatt ctcaccgcag ctattttctt ctctgtgttt
     2341 gcgacgtcac gtgcacagaa tcccaccatc ctcacgaacg tttcgcctta ttcacgctac
     2401 ggcatcggcg acatccagtt caaccagggg atgttcaacg ccggcatggg cggcggcggc
     2461 atcggttacc gcaacgattc gctcattccg caatacatca acctgcagaa cccggcctcg
     2521 tacacgggtc accagatcgt cgcttacgaa gtttcgcttc tcagcaacac ggttcagctg
     2581 cagaattcta ccacaacggg cacattcaac cgcacaacgc tcggaaattt cagcatggcg
     2641 tttcccgtta cgaaatggtg gggagccggg ttcggactta caccctattc gaacgtcggc
     2701 tacaaccttt cgctttccga caatcctgcg aatatcggac cggtcaccta caaatacgaa
     2761 ggctccggcg gcgtcgacca ggtgttcatc acgaatgcgt tccgcccgtt cgcaggtgcg
     2821 ccgagaaaat tcctgctgtc ggacagctac gacaagctga agctggcgaa cgacacttca
     2881 gccatgcagc gaaagctgaa gatgcgcaat gcgctcgcca atatttccgt cggtgtgaca
     2941 tcgtcctacc tgttcggaac acttaattac gtgcgccgcg acgaattccc ggattcactt
     3001 tacaacttca acacgaagat cagcaagcgt acgcagttcc gcgatttcta cctgaattac
     3061 ggcgtgcagt atacgttccg tctcccgaaa tcgcttaacc cgctttacca ctcgctgcct
     3121 gattcggtga tcacggggaa gaaaattttc agcaactatt tttcctacac gctgcacggt
     3181 aaatccgaca cggcaaagct tttcatccgc aaaccgggca ttcgcgtttc ggtcggtttt
     3241 gttttttcgc ttccgacaga catcaatgtt tcttccgaca tccttgcgca gacttacaag
     3301 ctgtacggaa ccatcgagca attccgcgat acggtgttca acaacacgaa cattccttcc
     3361 cgcatcgtga tccccgcgat gtatggcgcc ggcttcgcgc tgaaaaaaga tttccgctgg
     3421 gtgttccagg ccgattatat gacgcagctc tggagccagt ccactatcat gggacaggat
     3481 cccggcctga aagattcgcg gcgcgtcacc gcgggcttcc agttccagcc gaagcaggca
     3541 ggacgcggaa atttcctggg cacaggacag taccgtgtag gcgtgaggta ttaccagacc
     3601 tacctcgatc tcatggacac gcgtctgacg gaaatgtcgg ccaatctttc gctcgcattc
     3661 caggttccgc acaacacgca catcggagaa cccgtttccc gcctgcaggt gacgttcgaa
     3721 tacggtatgc gcggcactac gtcgaacaat ctcgtgcagg aaaatttcta tcgcgtgacg
     3781 ctgggactca ccatcaacga ccgctggttc aatcattata aatacgacta gcgcaggagc
     3841 gccatgtctc tttcaaaaac gtatttgctt tttgctttcg ccggatgttt cctgctgcat
     3901 gcgtgtgcca atgatcctgc gaaagtgaaa gcgttcacac gcgacgacag gcagcccgtg
     3961 cagacggcgc acgatgtgga catgctttac acggattccg cgaagctcaa gatccacatc
     4021 acttcaccgc aggtgaagga ttttgcggga agcaaaccgt acacggaaat gccggaaggc
     4081 gtaaaggttg aattcttcga tgatgcgcaa accgtgaaca gttacctcac gtcgaactac
     4141 gccatacgga aacagcgcga gcagcagacc gaagcgagga acgatgttgt cgtggtgaac
     4201 atcaaaggcg aaaagctgaa tacggaaaaa cttatctggg acgaggcaaa gcacaggatc
     4261 tacacggacg cattcgtgaa gatcacgacg aaagaccagg tgctgatggg aaacgggctc
     4321 gattcggatg agtcattcac caattacgag atcaaggaca tcacgggaag cttcacactc
     4381 aaagatcccg acaaaaaaca accatgaaaa agtatttgcg cctgaccgaa tattcctggc
     4441 ttttcctggc gatcatctgc atcgggatca ccacttattt tttcatcatc cacgacaccg
     4501 acagcggatc gatcgggctg atgctgacgc ttttcgccgg catcatgtac aatatgcggc
     4561 ggatgttcaa ccgcaggatg gaacgcatgg cgaagaaaca ggaagaggag cagcaaaagc
     4621 aataacctcc tgcaattttt cggcgaaagc caaacacttg cacctcagcg tattaattcg
     4681 ttattttttc tgaattagca gggcatattc cctgctaaat cgtatattcg tgcccctatt
     4741 caaaatcagt aaagtcatta cagtatgacc atcttgtcca gaatccgcag ccgtatcggc
     4801 ctgcttgtgg gaattatctt cctcgctctc cttgctttcg tattaaccga ccttttcaat
     4861 tcccagcgtg gaattttcgg cggacagtcc agcggcgccg tcggcgagat caacgggcat
     4921 tccatttcgc tcggtgaatt ccgtacgcgt cttgacgaag cgtccaatgg ccagcagctg
     4981 aacgatcagc agcagcagca gctttccgaa gccgtatggc aggagctgct cgaccagtac
     5041 atttacaaac cgcagtacga cgatctcggc atcgcgattt ccgatgatga gctcaccgag
     5101 cagatgacaa gcgaccatcc ttcggcttac atgcagcagt tcttccagga tcgccagtcg
     5161 ggacagatca tgcagcagtt ccagggacct gacgggcagc tcagcggaaa agccatccgc
     5221 gatttcgtcg cgaagatgcc ggccgaaacc gagcagcagt gggtgcagat cgaaaaagac
     5281 atgcgcaaaa cgctgctccg cgaaaaatac aacaacctcc tccgcaaagg attttacgtt
     5341 accaccgcag aagcgaagca cgagtacgcg gatgaaaaca cgaaatacga tttcaatttc
     5401 atcgtaaaaa aatacagcga gatccccgac tccaccgtca aggtcaccga cgacgagatg
     5461 atggattact acaaccagca ccagtacaag ttcaagcaga cggacaacat gcgcagcatg
     5521 gaatacctcg cgttcgatgt ttatccttcc gccgatgaca tcgcggatca gcgcaaggac
     5581 atggaaaaca tggtgaccga attccagaag cagaaaaccg ctgccgatga ttccatgttc
     5641 gcagtgagca tgaacgaaag cggaacgttc gataaaaaat acctccgccc cgggcaattc
     5701 ccggtcggtt ccgacagcgc tttcctgaaa gcaaaaaatg gcgatgttct cggaccgttc
     5761 aacatgggcg gcaacctcga catttacaaa gtggtcgggc aggataattc acccgactcc
     5821 gccaaagtgc gccacattct tattgcgtac aaaggcgccg accgtgcggg gccggagatc
     5881 accagaacga aagaacaggc gaaaatgcgc gccgacagta ttctcaaagt cgtgaagagc
     5941 ggccgcacca agctggaaga tctcgtatgg aaactgacgg atgacccggg ctccaaagat
     6001 cagaatgcaa aacccgacgc gcccggttac aaaggcgact acggctggtt tacccatgaa
     6061 agcggtttcg tgcaggagtt caaagacgcg ggcttcaaca atcctgttgg ttcgaccatc
     6121 gtcgttgaaa cgtcgttcgg ttaccacgtc atccaggtgc tcgaccgcac aaaaccgacc
     6181 acgaaagtgc aggtgatcgc gatcgagaaa aaagtggagc catccgaaaa gacgatccgg
     6241 atcgtttaca acaaagcctc cgaattcgcc ggcaagaaca acaccggtga tctcttcacc
     6301 gcagccgttc agaagaacag catgcaggtg ctgaaagccg accagatcca ggaaaattca
     6361 cggtacatca gcggcattga aaacgcacgc gatatcgttc gctggatgta tgacgataaa
     6421 acagaagtgg gttccgtttc gcagcctttc cagtcgggcg accgctacgt ggtgtgccac
     6481 ctcacgaaga tcatcaacaa aggcgtcaag cccttcgatg aagtgaaaga catctgccag
     6541 ctcgaagcgc gcaagatcaa aaaaggacag atgttcactg accagctcaa caaagcgaaa
     6601 ggcgctacgc tcgacgcatg ggcgaaaaac gtgggcacga cggtaatgcc cgccgtgaac
     6661 atcagcttcg ctgcacctta catccaggct gcaggttacg aaggcactgt ggtcggtgtt
     6721 gcatcaacgc ttaagcccaa cacgctttcc gcaccgatca aaggttctat gggcgtctat
     6781 gttgtgtcgc tgacaaaagt gaccaaagca gatcccctgc tcgacatcgc cggtgaaaaa
     6841 gcgaaagttc tccagaacaa ttattcaggt cgcgccgacg gcgtggcgaa tgaagtgctg
     6901 aaagacgacg cgaatgttgt cgacaaccgc gcgaaacatt tctgagctta actcttttgt
     6961 gcgaaaagcc ttcccgttcc cggggaggct tttttgcttc cgggcggaga gatcccgctg
     7021 ctaccttttt cacgacgatc ctggtatttc attttccggt cgcgacgttc cacgtacacg
     7081 ttttcttttt ccaggtccgc tgtttcaagt cgcgtgttta aagtgaaaga cagctttcct
     7141 gcccggaaag atcagcgcag cacgagcagc cgctgcgaat cgagcttgat gaagatgaag
     7201 agcaggatcg tgaaaccgat gaggctcgac ccgccatagc tgaagaacgg aagcgggatg
     7261 ccgatcaccg ggataagccc gatggtcatc ccggtgttga caataaggtg aatgaaaaat
     7321 atggctgc
//
LOCUS       DASTSF010000380         3867 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_730524,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000380 DASTSF010000000
VERSION     DASTSF010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3867
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_730524"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..585)
                     /locus_tag="VFU15_10905"
     CDS             complement(<1..585)
                     /locus_tag="VFU15_10905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718336.1"
                     /translation="MRSASSRAFLKTTGRVFLSLLLLCAAQKSFAQHLYIAHDFENCG
                     VGLKDSTTGQWVVQPTYVNIYPLGKFFKSFDGNKVGLISPSGKQIVIPKYDNIFPKRD
                     SATPYFTFYDHRREGLIDDSGRVVLPARYSITVTYRDSAFAFRRHGRTRLYMPGKTPI
                     KIHSHGVPEYIGNGLFIVNKSYRKYGLIDSTGKTI"
     gene            complement(895..2160)
                     /locus_tag="VFU15_10910"
     CDS             complement(895..2160)
                     /locus_tag="VFU15_10910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017731446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthetase B"
                     /protein_id="HEU4718337.1"
                     /translation="MKRISLFLFLSGFALRMHAAYILIPMDDAQKDHLKAYGVTYYVL
                     QQGGEAQWLLNYRYGSFLFKYDKDFETRCLTRGVTYETIPDAKANSILSDISSPDVNE
                     DAVKLEKAPKIAVYSPPNKQPWDDAVTLVLTYAEIPYRVIYDEQVMQDSLLLYDWLHL
                     HHEDFTGQYGKFYRGFSQADWYKQQVATDEAMAAKLGFNKVSQLKLGVCKKIRDFVQN
                     GGFMFAMCSATDTYDIALAAEGVDICDKIYDHDPADPDMNSKLDYSKCFAFQNFKLKT
                     DPLEYEFSDIDTYETRMFKGVTENNDYFSLFEFSAKWDPVPTMLCQDHANLIKGFWGQ
                     TTGFDKAKLKPNVLIMGENKAINEARYIHGDYGKGMWTFYSGHDPEDYQHQVGEPPTD
                     LSLYPNSAGYRLILNNVLFPAARKKHLKT"
     gene            complement(2365..>3867)
                     /gene="dnaB"
                     /locus_tag="VFU15_10915"
     CDS             complement(2365..>3867)
                     /gene="dnaB"
                     /locus_tag="VFU15_10915"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016196345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEU4718338.1"
                     /translation="EIGKLPPQAVELEEAVLGALMLEKDALTAVIDVLHPKIFYKEAH
                     GRIFNAIHNLFQRSEPIDILTVTSELRRTGELEIVGGAYFISQLTSRVASSANVEFHA
                     RIIAQKYIQRELINISSETIKEAYEDTADVFDLLDSAERKLFSVVEGNIRKNYDKMSS
                     LISQALKNIEAARDQKSGVTGVPCGFTDLDRMTSGWQNSDLIIVAARPAMGKTAFVLS
                     MARNAAVEFTKPVAVFSLEMASVQLVQRLISSESELPAEKLKKGQLEDHEFQQLHVKI
                     GKLSEAPLFIDDTPALSIFEMRAKCRRLKAQHDIQLIIVDYLQLMTTGSEHNRNGNRE
                     QEISTISRSLKSIAKELNVPVIALSQLSRAVETRGGDKRPQLSDLRESGAIEQDADMV
                     LFIHRPEYYGFTQDPEGNNTAGLAEIIIAKHRNGAVGSVNLRFIDRLAKFMDWGGDHL
                     GDSGAGYYTNSGDGNYNPAAGITPAGDFIPPSTGNIIRGSKMNDIEEDPF"
BASE COUNT          837 a         1003 c         1086 g          941 t
ORIGIN      
        1 gatcgttttg cccgtgctgt cgatgagccc gtacttccgg taggatttgt tgacgatgaa
       61 caggccgttg ccgatgtact cgggtactcc atgactatgg atctttattg gcgttttgcc
      121 gggcatgtag aggcgtgtgc gaccatgacg gcggaaggcg aaggcggagt cccggtaggt
      181 aacggtaatg ctgtacctcg ccggcagcac gacccgcccg ctgtcgtcga tcagtccctc
      241 gcgccggtga tcgtaaaaag taaaataggg agtggcggaa tcccgcttgg gaaagatgtt
      301 gtcatacttc gggatcacga tctgctttcc gctgggagag atcagcccca ctttatttcc
      361 gtcgaacgat ttgaagaatt ttcccagcgg gtagatgttc acgtaggtag gctgaaccac
      421 ccattgtccc gtcgtgctgt ctttcaatcc cacgccgcag ttttcaaaat catgcgcgat
      481 atacaggtgc tgggcaaaac ttttttgcgc cgcacaaagc agcagcaggg aaaggaaaac
      541 tcttcccgtt gttttcagga atgctctgga actcgcggac cgcatgagga cgatttaggg
      601 tataacgggt tgtttaatta aagatacagg attcgatatt gcgtttaagg ttgccaatgt
      661 tttcaaccgg gttctttttt gccccggaga ttatgaaaac agaattaaga cgcagggagg
      721 agatccggag gcttttgcgt ttttcaatga cgggttttaa tacagggaag catgggatcg
      781 tttcacgcgg atgcacgaaa aataaaaggc tgcgaaggaa accggtaacg gaaaacaatt
      841 caaatcccca acgacagcag aaaatccgac atccgaaatc cgacatccgg gacgtcaggt
      901 tttgagatgc tttttcctcg ctgccgggaa taaaacgttg ttgaggataa ggcggtagcc
      961 cgccgaattg ggataaagcg aaagatctgt aggcggctct cccacctggt gctggtagtc
     1021 ttccggatcg tggccgctgt agaacgtcca catgcctttt ccgtaatcac cgtgaatgta
     1081 acgcgcttcg ttgatcgctt tgttctcgcc catgatgagc acgttcggct tcagtttcgc
     1141 cttgtcgaaa cctgtcgtct gtccccagaa tcctttgatg aggttcgcat ggtcctggca
     1201 cagcatcgtg ggaacaggat cccatttcgc ggaaaattcg aagagcgaaa aataatcgtt
     1261 gttctccgtc acgcctttga acatgcgcgt ttcgtaggtg tcgatatcgg aaaattcata
     1321 ttcgagcgga tcggttttca gcttgaaatt ctggaacgcg aagcacttcg aatagtcgag
     1381 cttgctgttc atgtccgggt cggccgggtc gtgatcgtag atcttgtcgc agatgtcgac
     1441 accttctgca gcgagcgcaa tgtcgtacgt gtcggtggcg gagcacatcg cgaacatgaa
     1501 gccgccgttc tgcacgaagt cgcggatctt tttgcagacg cccagcttca gctgggaaac
     1561 tttgttgaag ccgagcttcg ccgccatcgc ttcgtccgtc gctacctgtt gcttgtacca
     1621 gtcggcctgc gaaaacccgc ggtagaattt tccgtactgc ccggtgaaat cttcgtggtg
     1681 caggtgcagc cagtcgtaca gcagcagtga atcctgcatc acctgctcat cataaataac
     1741 acggtacgga atttccgcgt acgtgagcac gagcgtgacg gcgtcgtccc agggttgttt
     1801 gttcggcgga gaatacacgg cgatcttcgg cgctttttcc agtttcaccg catcttcatt
     1861 cacgtccggg ctcgaaatgt cgctgaggat ggaattcgct ttggcgtcgg gtatggtttc
     1921 gtaggtcacg ccgcgtgtga ggcagcgcgt ttcaaaatct ttatcgtatt tgaaaaggaa
     1981 gctgccgtaa cggtaattca gcagccattg cgcctcgcca ccctgctgca aaacataata
     2041 cgttacgccg tacgctttca ggtgatcttt ctgtgcgtcg tccatcggga tgaggatgta
     2101 cgccgcatgc atccgcaatg cgaagccgga caggaaaaga aaaagtgaaa tgcgtttcat
     2161 gccaaatgat ctttaaagtt acaaagaccc ggcgaaacaa tggtcattgg agagctgtta
     2221 aaaaatgcaa ggtgcgtaaa cgggaaaatt gcgctgcgaa atcatgcgcg gtcaaaacgc
     2281 gttgcctgga aaaatagccc ggcccggatt ctcataagta cctgcgttat ttacgatcgc
     2341 aaatcgtgcg ccgtagatcg tcgatcaaaa aggatcttcc tcgatatcgt tcatcttcga
     2401 tccgcggatg atgttccccg tggaaggcgg aatgaaatca ccggcgggtg tgatgccggc
     2461 tgccgggttg taatttccgt cgccgctatt ggtgtaataa cctgcgccgc tgtcgccgag
     2521 atgatcgccg ccccagtcca tgaatttcgc cagccggtcg atgaagcgga ggttcacgct
     2581 gcccacggcg ccgttcctgt gcttggcaat gatgatctcg gcaagacctg ccgtattgtt
     2641 cccttcggga tcctgggtaa agccgtagta ttccgggcgg tgaatgaaca gcaccatgtc
     2701 ggcgtcctgc tcgatcgcac ccgattcacg aaggtcggaa agctgcgggc gcttgtcgcc
     2761 gccgcgcgtt tcaacggcac ggctcagctg ggaaagagcg atcaccggga cattcagctc
     2821 cttcgcgatg cttttcagcg aacgcgagat cgtgctgatc tcctgctcgc ggttgccgtt
     2881 gcggttgtgc tcgctgcccg tggtcatcag ctggagataa tcgacgatga tgagctgtat
     2941 gtcgtgctgc gctttgagac ggcggcattt tgcacgcatc tcgaaaatgg aaagcgcggg
     3001 cgtgtcgtcg atgaaaagcg gcgcttcgga aagcttgccg atcttcacgt gcagctgctg
     3061 gaactcgtgg tcctcgagct gtcccttctt cagtttttct gccggcagtt ccgattcact
     3121 ggaaataaga cgctgcacca gctgaacgga ggccatctcg agcgagaaca ccgccacagg
     3181 tttcgtgaat tccaccgccg cgttgcgcgc catcgaaagc acgaacgctg ttttacccat
     3241 cgcgggacgt gcagccacga taataagatc cgaattctgc cagcccgacg tcatgcggtc
     3301 gagatcggtg aacccgcagg gaacgcccgt cacacccgat ttctggtcgc gcgccgcttc
     3361 gatattttta agcgcctggc tgatgagcga gctcatcttg tcgtagttct tgcggatgtt
     3421 gccttcgacg acagagaaaa gtttccgctc cgcgctgtcg agaaggtcga acacatcggc
     3481 cgtgtcttcg tacgcttcct tgatcgtctc gctgctgatg ttgatcagct cgcgctggat
     3541 gtatttctgc gcgatgatgc gcgcatggaa ttccacgttg gcggaagacg ccacacgcga
     3601 cgtaagctgt gaaatgaaat aggcgccgcc gactatctcg agctcgcctg tgcggcgcag
     3661 ttcactcgtg accgtaagga tatcgatcgg ctctgaacgc tggaaaagat tatggatggc
     3721 gttgaaaatt cttccgtgcg cttccttgta aaaaatcttg ggatgcagca cgtcgatgac
     3781 ggcggtgagc gcgtctttct ccagcataag cgccccgagc acggcttcct ccagttccac
     3841 ggcttgcggc ggtagcttgc cgatctc
//
LOCUS       DASTSF010000381         3190 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1401695,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000381 DASTSF010000000
VERSION     DASTSF010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3190
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1401695"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1214)
                     /locus_tag="VFU15_10920"
     CDS             complement(<1..1214)
                     /locus_tag="VFU15_10920"
                     /inference="COORDINATES: protein motif:HMM:NF039901.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="contractile injection system tape measure
                     protein"
                     /protein_id="HEU4718339.1"
                     /translation="MGKQNHIIRKLVIEIGLPTQEEAFRIQNRLTDEYRRIILAIIEE
                     ILDRLVREEEIITVDRLEIDLGHVTAAHLSEEISRKVKQEMEEAVTNLLYELRSGSAG
                     AAGIRMKTSRGDSITVQARRSHTAESSLETLAYFLEYGIFPWSEDRKKKPSLREMIGE
                     ALEKRPEELLRMLAQSVTKKHVFRRLALQLPEDQLCYLCAVLGCSFSAQLSQVFSGLE
                     KIIRLLLREISRTGIQLPFPPEGSELHFRIREEALRYFASGEKWQGDLFLPGKVSDPV
                     VKYFAVMFFSIIGKSALAVSKLPVPRSGNRPGGKSRKRNPVDGNFAKEISEALALFAP
                     RGEKQTASKSRKKKKNAGRKAAPGAEEEKREVAAEEKSTPLQYAEEETPPAEVPAEDG
                     IYVANSGLIILHP"
     gene            complement(1266..>3190)
                     /locus_tag="VFU15_10925"
     CDS             complement(1266..>3190)
                     /locus_tag="VFU15_10925"
                     /inference="COORDINATES: protein motif:HMM:NF037016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEU4718340.1"
                     /translation="FWNFENIRRNKDVSGQYPVVAYGNQSNNQTAAIDPIQTPNFYDL
                     DAFSFLRIEGHLRKNKTDVITDLNTQKANFDLPFNVVAVRLQGTSTPDDILGRCNFND
                     LRSQYVAYRGEMYCFIQRLFDHFFEVVQTDSTAGIPGSTSVKPRQAPKWIRSLLQDTN
                     SPTSQGNSSALFLPAYYALPGQQLPHIAQPFVYSPTPLSQLISIDLNNYLTSLASRLT
                     QLGLLLPDQLDDFDFGADNQLDVDNSFIGSYIDAVSLCNILKALFNEISDQVTHSSKN
                     RFTQEQYFMFGQWLSEQMYFLNEFITDCKYNKLAAVFYEMQYRINYLQTNDPTVFSNF
                     ITRNPGIIHGAGTTPGGTFVLVYPGATLNFSIKVKEQLAAVLYDIQVKEIRRNELRAI
                     RGRSIEQNTELIKLDSQLCDLYAVQVQQPVRPVSAGIAISAIPVQQISIDQDEVIADF
                     WLPYLSNCECECDDIPAPTTAQLGIPAIAMPSFYEFFLGDYAFAKDIITNTFGCTTPA
                     QLTIDIRPSVNYTQIDLKEPNFKLKFIVNGDVQNRDINVLSRSSSTTTTSAKGGTVLI
                     NPGQSNYDVFVYTPPANFLGTDSFEYVFELYDRSNNILLHSNKAKVTIVVTTRCKPAT
                     AVTNSAAVFNEVPATT"
BASE COUNT          666 a          815 c          903 g          806 t
ORIGIN      
        1 ggatgaagga tgataagtcc tgaattagct acgtatatgc catcttctgc cggaacctct
       61 gccgggggag tttcttcttc tgcgtattgc agcggcgtgc ttttttcttc agccgcaacc
      121 tcacgttttt cttcctctgc acctggtgct gctttccggc cggcgttctt ctttttcttc
      181 ctgcttttcg acgcggtctg cttttccccg cgaggagcaa agagagcaag cgcttcgctt
      241 atttctttcg caaagttgcc gtcaacaggg tttcttttcc gggattttcc tccgggcctg
      301 ttcccggaac gaggcaccgg aagcttactc actgcaagcg cggatttgcc gatgatgctg
      361 aagaacatca cagcgaaata cttcacaact gggtcgctta cttttccggg aagaaaaaga
      421 tctccttgcc atttttctcc tgaagcgaaa taccgcaacg cctcttcccg gatacggaaa
      481 tgcaattcac ttccttccgg cggaaaagga agctgtattc cggtgcgcga aatttcccgg
      541 aggagcaaac ggataatttt ctccagcccg gaaaaaacct gggaaagctg cgccgagaaa
      601 ctgcacccga gaactgcgca gagatagcac agctggtctt ccggcaactg cagggcgagg
      661 cgccggaaaa catgtttttt agttacgctc tgcgccagca tgcgcagcaa ttcctccggg
      721 cgtttttcaa gcgcctcacc gatcatttcg cggagcgaag gctttttctt ccggtcttcc
      781 gaccagggaa agatcccgta ttcaagaaaa taagcgagcg tttccagtga agattctgca
      841 gtgtgactcc gtcttgcctg cacagtgatg gaatcgccgc gggaagtctt catgcgtata
      901 ccggcggctc cggctgaacc ggaccgcaat tcatagagaa ggttcgttac cgcttcttcc
      961 atttcctgct tcaccttgcg ggaaatttct tccgaaagat gcgcagccgt gacgtggccg
     1021 aggtcgatct cgagcctgtc aacggttatg atctcctctt cacgcaccag cctgtcgagg
     1081 atttcctcga tgatggcaag aataattctt ctgtattcgt ccgtcagccg gttctgtatc
     1141 cggaacgctt cttcctgcgt gggcaaaccg atctcgataa cgagcttcct gatgatatgg
     1201 ttttgtttac ccatgcttta ctgaatgact gctgacgcat ccggtgacgt gattatgttt
     1261 ttttctcagg tggtagccgg aacttcattg aatacggcgg cggagttggt gaccgccgtg
     1321 gccggcttgc agcgcgtggt caccacgatg gtcactttcg ctttgttcga atgcagcagg
     1381 atgttgttgc tgcggtcgta cagctcgaac acgtattcga acgaatccgt tccgaggaag
     1441 ttggccggcg gtgtgtacac gaacacgtcg taattcgact ggcccgggtt gatgagcacg
     1501 gtgccgcctt tcgccgatgt cgtggtggtg gaagaggaac ggctcagcac gttgatgtcg
     1561 cggttctgca cgtccccgtt cacgatgaac ttcagcttga agttcggttc cttgagatcg
     1621 atctgcgtat agttgacgct cggccggata tcgatcgtga gctgcgccgg tgtcgtgcag
     1681 ccgaaggtgt tcgtgatgat gtctttcgcg aacgcgtaat cgccgaggaa gaactcgtag
     1741 aacgacggca ttgcgatggc cggaatcccg agctgtgctg tcgtcggtgc gggaatgtcg
     1801 tcgcattcgc attcgcagtt ggaaagatat ggcagccaga aatcggcgat gacttcgtcc
     1861 tggtcaatgg agatctgctg caccggtatg gcggaaatag cgatgcctgc tgaaaccggg
     1921 cgcacaggtt gctgcacctg cacagcataa agatcgcaga gctgtgaatc gagctttata
     1981 agctctgtgt tctgctcgat gcttcttccg cgtatcgcgc gcaattcgtt ccggcgtatt
     2041 tccttcacct gtatatcgta gagaacggct gccagctgtt ctttcacctt gatggagaaa
     2101 ttcagcgtcg cacccgggta aacgagaacg aatgttccgc ccggcgtggt tccggctccg
     2161 tgaatgatgc cgggattgcg tgtgatgaaa ttcgagaaga ccgtcggatc gttcgtctgc
     2221 aggtaattga tgcggtactg catttcgtag aataccgcag cgagcttgtt gtacttacag
     2281 tcggtgatga attcattgag gaaatacatc tgttcgctca gccattgccc gaacatgaaa
     2341 tactgttcct gcgtaaaacg attcttcgag ctgtgcgtaa cctggtcgct gatctcgttg
     2401 aacagcgctt tcaggatatt gcaaagcgaa accgcgtcga tataggaacc gatgaagctg
     2461 ttgtcgacgt ccagctggtt gtcggcgccg aaatcgaaat cgtcgagctg gtcaggcagc
     2521 agcagaccca actgcgtcag gcgtgaagcc agcgaagtga ggtaattatt caggtcgatc
     2581 gagatcagct gtgagagcgg cgtaggggaa tacacaaacg gctgcgcgat gtgcggcaac
     2641 tgctggccgg gcaatgcata gtacgcggga aggaagagcg ccgaagaatt tccctggctc
     2701 gtcggcgagt tggtatcctg cagcaatgaa cggatccatt tgggcgcctg ccgcggcttc
     2761 acgcttgtcg aaccgggtat gcctgccgtg ctgtcggtct gcaccacttc gaagaaatga
     2821 tcgaagaggc gctggatgaa gcaatacatt tctccgcggt aagcaacgta ctgcgagcgc
     2881 aggtcgttga aattgcagcg accgagaatg tcatcgggtg tcgaggttcc ctgcaggcgc
     2941 acggcgacta cgttgaacgg aagatcgaaa ttcgctttct gcgtattaag atcggtgatg
     3001 acgtcggtct tgtttttgcg caggtgacct tcgatgcgca ggaagctgaa cgcatcgaga
     3061 tcgtagaagt tcggcgtctg gatgggatcg atcgctgccg tctggttgtt cgactggttg
     3121 ccatacgcca ctaccgggta ctgcccgctc acatctttgt tgcggcggat attttcgaag
     3181 ttccagaatt
//
LOCUS       DASTSF010000382         4327 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1068284,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000382 DASTSF010000000
VERSION     DASTSF010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4327
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1068284"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1826
                     /locus_tag="VFU15_10930"
     CDS             <1..1826
                     /locus_tag="VFU15_10930"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEU4718341.1"
                     /translation="TPLGGLQFQLVTSLALTDRSPNSSHFTGNLYVSQVDVPAIRDMD
                     GDGDLDVLTFQNAGTEIEYHRNMGMELYHTCDSIHFQVESNCWGGIAEDALNAAISMG
                     VTCPNPPIVEDFTQYSAQHSAAHSGSCLECINTDGDDDQDVLIGDISYAHSTFVHNGG
                     SAQSALIDYVDSTFPSYNVPVNQQIMPCGFHLDVNNDGKKDILFSPNTGYSSDNYASI
                     AYYMNVGDNDSVVASYVQNNFLQDNEIEVGEGCYPVFFDYDNDGDKDLFIGDLGYYNI
                     AGPLISKIALYKNTGTSTHPVFSKVTNDFANIYANGSGIVGLAPCFADLDGDGDKDML
                     VGAADGRLYYFEKMPGPDDNFVLSQAFYQNIDVGSNAVPQLIDVDHDNKVDLLVGNQA
                     GRVYYYHNTGTSAAPAFTQASNFFGGIDVRQPAYYTGYAVPRLYMNNGQYEMLVGSER
                     GYIYKYDDIDNNLSGTFNLVDSMYISTREGGQLSPYIDDINGDGLYDVIIGNFAGGAS
                     IFMGDNNVSTGELAGHEKGSFNLYPDPANDFVTIEFPNGLFSSGNRIDIFNIAGQCVY
                     TTVSGSARTTVPVAGLSPGVYICSVTDPAGARKNKKLIITH"
     gene            1828..4038
                     /locus_tag="VFU15_10935"
     CDS             1828..4038
                     /locus_tag="VFU15_10935"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEU4718342.1"
                     /translation="MKLRILSAFLFFTASLFSQQQYLRQDTVSVFAGNHRQPYAWAGG
                     MNSCQYSDIDLNQDGIMDLFVYDRSLYKITTFLNLGTANQPSYVYAPQYIPAFPGMKD
                     WCLLRDFDGDGKSDIFTGNANMVKVYRNVSTTQTGLAFQVYTNEVRADITPNSPPTYD
                     AIFVSWIDVPAIRDVDGDGDLDILNYGTGGIQVEYYKNMSEEMYGNADSLNYVLTTLC
                     WGEYSESQLDATITLHTPCPQPPLVKNPDVTHNPMLHNGSCLECINTDGDNDQDLLVG
                     DLSNYYINYIRNGGTSSYAIGDSVDDHYPAYDTVANIIAFGCGYHVDVDNDGNKDVLF
                     SANSMSNSQNWNSSWYYHNTGTNNSVHLSFVQRDFLQGGMIDVGEGAYPVFFDYDHDG
                     DPDLFLGNRGYSDASGVMRSSIALYKNIGSSTAPMFSLVSSDFGGFYGTDSLIIAGFA
                     PTFGDIDGDGDADMITGDVNGKLQYFRKDPGSDTNFVYDTPNYMGIDVGSNATPQLID
                     VDHDGLLDLLIGAQSGNVNYYRNTGTASSPNFTLVTNMFGNIMVTETGFITGYSAPCL
                     FMHNGAYEMYVGSERGYVFHYTNIDGNLSGNFTLSDSLSVSHYEGGRCVPFVADVNGD
                     STMDIIIGNYAGGISLFFGDQNFSVQEHLPSFMVYSLYPNPANGSFTIGTTVTPGDPK
                     TLSLYSVSGSLLLEEKIVAGKTIIDTKDLPPGIYFCTVRDASGFANTQKLVINR"
     gene            4043..>4327
                     /locus_tag="VFU15_10940"
     CDS             4043..>4327
                     /locus_tag="VFU15_10940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEU4718343.1"
                     /translation="MKKLIPLLLLFSATEISGQQQFFRYDSIPVSQNSQSCMFGWAGG
                     VNFAQFSEIDLNQDGVMDLFVFDRTGNKISTYINNGTAGQVDYHYAPEYIP"
BASE COUNT         1019 a         1225 c         1094 g          989 t
ORIGIN      
        1 ccaccccgct gggcggactc cagttccagc tcgtcacttc gctggcgctt accgaccgtt
       61 cgccgaacag cagccatttt acggggaatt tatatgtaag ccaggtggac gtgccggcca
      121 tccgcgacat ggacggggac ggggacctgg atgtgctgac tttccagaac gccggaacgg
      181 agatcgaata ccaccggaac atgggcatgg aactttatca tacgtgcgac agcattcact
      241 tccaggtgga atcgaattgc tggggaggaa ttgccgagga cgcgctgaat gcagcaataa
      301 gcatgggcgt gacctgcccc aatccgccga tcgtggaaga tttcacgcaa tattccgcgc
      361 agcactcggc cgcgcattcc ggttcatgcc tcgaatgcat caatacggac ggtgatgacg
      421 accaggacgt tcttatcggc gacatttcct acgcacacag cactttcgtc cataacgggg
      481 gcagtgcgca aagcgcgctg atcgattacg tggattccac cttcccttcg tacaacgtcc
      541 cggtcaacca gcagatcatg ccctgcgggt ttcacctcga cgtgaacaac gacggcaaaa
      601 aagacattct cttttcaccg aacaccggtt attcatcgga caattacgcc agcatagctt
      661 attatatgaa tgtcggcgac aacgattccg tcgtggcttc gtatgtacag aataattttc
      721 tccaggataa tgagatagag gtcggtgaag gatgttaccc tgtatttttt gattatgaca
      781 atgacgggga caaagatctt tttatcggcg acctcggtta ctacaacatt gcaggtccgc
      841 tgatctcgaa gatcgcgctg tataaaaaca ccggcacttc cactcatccc gtattctcaa
      901 aggtcaccaa tgatttcgcc aacatttatg cgaacggatc cggaattgta gggcttgcac
      961 cctgcttcgc cgatctcgac ggagacgggg acaaggatat gctggttggt gcagcggacg
     1021 ggcgattgta ctatttcgaa aaaatgcccg gtcctgatga caacttcgtt ctgtcgcagg
     1081 ccttttacca gaacatcgac gtgggcagca atgccgttcc gcagctgatc gacgtcgacc
     1141 atgacaacaa ggtcgacctg ctcgtcggca accaggcggg acgcgtttat tattaccaca
     1201 acacgggaac gtccgccgcg ccggcattca cgcaggcgag caatttcttc ggcggaattg
     1261 acgtgcggca acccgcgtat tatacgggct atgctgtccc ccgtctttac atgaacaacg
     1321 gccagtatga aatgctcgtc ggttccgagc gcgggtacat ttacaaatac gacgacatcg
     1381 acaacaacct ttccggcacg ttcaacctcg tcgattccat gtacatctcg acgcgcgagg
     1441 gaggacagct ttcgccttac atcgacgaca tcaacgggga cgggctgtac gacgtgatca
     1501 tcgggaattt cgcaggcggc gcttccatat ttatgggcga caacaatgtt tcgacgggcg
     1561 agctggcggg ccatgaaaaa ggcagcttca atctttaccc cgatcccgcg aacgatttcg
     1621 tcaccatcga atttccgaac gggcttttct cttccgggaa ccggatagac atcttcaaca
     1681 tcgcggggca atgcgtgtac acgactgtca gcggatctgc gcggacaacc gtgccggtag
     1741 ccggtctttc acccggagtt tacatttgtt ccgtcaccga tcctgccgga gcgcgcaaaa
     1801 acaaaaaact catcattacc cattgaaatg aaattacgca tcctttccgc tttccttttt
     1861 ttcaccgcct cacttttttc acaacaacaa tacctgcgcc aggatacggt ttctgttttc
     1921 gcaggcaatc accggcagcc atacgcatgg gccggcggaa tgaattcctg ccagtattcc
     1981 gatatcgatc tcaaccagga cgggatcatg gatcttttcg tttacgaccg ctcgttgtac
     2041 aagatcacca cttttcttaa cctgggcacg gccaaccagc cctcgtacgt ttacgcaccg
     2101 caatacatcc ctgcgtttcc gggcatgaaa gactggtgcc tgctgcgtga tttcgacggg
     2161 gacggcaagt cggatatttt taccggcaac gccaatatgg taaaagtgta caggaatgtt
     2221 tccaccacgc agaccgggct tgccttccag gtttacacca acgaggtccg ggcggacatt
     2281 acgccgaaca gtccgcccac gtatgatgca attttcgtga gctggatcga tgttcccgcg
     2341 atccgtgacg tggacggcga cggcgacctc gacattctca attacggtac cggcggcatc
     2401 caggtggaat attacaagaa tatgtcggag gaaatgtacg ggaacgcgga cagcctgaac
     2461 tatgttctca ccacgctctg ctggggtgaa tattccgaaa gccagctcga cgcaacgatc
     2521 acgcttcaca cgccctgtcc ccaaccgcct ctcgtaaaaa atccggacgt aacgcacaac
     2581 ccgatgctgc acaacggttc ctgcctcgag tgcatcaata cggacggcga caacgaccag
     2641 gacctgctcg tgggcgatct ttccaactat tatatcaact acatccgcaa cggcggaacg
     2701 agctcctatg cgatcggcga ttccgttgac gaccattatc ccgcttatga cacggtggca
     2761 aacatcattg cattcggatg cggctatcac gtcgacgtcg acaatgacgg gaacaaggac
     2821 gtgctctttt ccgcgaacag catgtcgaat tcgcagaact ggaacagctc gtggtattac
     2881 cacaatacag gaacgaacaa ctccgtgcat ctttctttcg tgcagcgcga ttttctccag
     2941 ggcggaatga tcgacgtggg cgaaggcgcc tatcccgttt ttttcgatta cgatcacgac
     3001 ggagaccccg atcttttcct cggtaaccgc ggttattccg acgccagcgg cgtcatgcgc
     3061 tcatcgatcg cgctgtataa aaatatcggc tcttccacgg cgccgatgtt ctcgctcgtc
     3121 tcttccgatt tcggcggatt ttacggaacc gacagcctca tcattgccgg ttttgcgccg
     3181 acgttcggtg acattgacgg ggacggcgac gcggacatga tcaccgggga cgtgaacggg
     3241 aagctgcagt atttccggaa agacccgggc agcgacacga acttcgttta cgacacgccg
     3301 aactacatgg gcatcgatgt cggctcgaac gccacgccgc agctcatcga cgtggaccac
     3361 gacggcctgc tcgacctgct gatcggcgcg cagagcggca acgtgaatta ttaccggaat
     3421 acgggcaccg ccagttcacc gaatttcaca ctggtgacga acatgttcgg caacatcatg
     3481 gtaacggaaa ccggtttcat caccggctac agcgcaccgt gccttttcat gcacaacggg
     3541 gcgtatgaaa tgtacgtggg cagcgaacgc ggctacgttt ttcactacac gaacatcgac
     3601 ggcaaccttt cgggaaactt cacgctctcc gattcgctaa gcgtgagtca ttacgaagga
     3661 ggacgctgcg ttcctttcgt agccgatgtg aacggcgaca gcacgatgga tattatcatc
     3721 gggaattacg caggaggcat ttctcttttc ttcggcgacc agaatttttc cgtgcaggag
     3781 catcttccat cattcatggt gtattcgctg tatccgaatc ccgcgaacgg gagctttacg
     3841 atcggaacga ccgtaacgcc gggtgatccg aaaacacttt ctctttattc cgtttccggg
     3901 agccttcttc tcgaagaaaa gatcgtcgcc ggtaaaacga tcatagacac aaaagatctt
     3961 ccgcccggca tttatttctg cacagtacgc gacgcttccg gctttgccaa tacgcagaag
     4021 cttgtgatca accgctgata cgatgaagaa actcattcca ctcctgcttc ttttttcagc
     4081 tacggaaatt tccgggcagc agcaattttt ccgctacgac agcattcctg tttcgcagaa
     4141 ttcccaatcg tgcatgttcg gatgggcggg cggcgtcaac ttcgcgcaat tttccgagat
     4201 cgacctgaac caggacggtg tgatggacct ttttgtcttc gaccggacgg gcaacaaaat
     4261 ttccacgtac atcaataacg gaacggcggg acaggtcgac tatcattatg cgccggaata
     4321 cattccg
//
LOCUS       DASTSF010000383         7880 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1811704,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000383 DASTSF010000000
VERSION     DASTSF010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 7880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7880
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1811704"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2283)
                     /locus_tag="VFU15_10945"
     CDS             complement(<1..2283)
                     /locus_tag="VFU15_10945"
                     /inference="COORDINATES: protein motif:HMM:NF018449.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HEU4718344.1"
                     /translation="MNKILLSFLFASFTVSAFATDIGFTENKGQFRTAGTKDYPANVL
                     FRSYGTSAAMYVTDHGIDYFFTKKEKTSDDNSVSVTGWSRIGMTLTGADIRKENIATE
                     DPLPGVTNYYLADCPQGITGVRSWHKVTIRNIYPGIDWVLTADNEKGVSHDFIVHPGA
                     DPALIRISYTGENLGDMKASDADLVFPTAYGSLNEGKLHVYEQGSGRVVPASFDILLD
                     LPGCMRKSSAAHAEVSYAISSYSVNETLVIDPPLQWSSTQASTSADYGYATIAAGDGS
                     GDVIVTGASDGTDFPVQNAYQGQLYGFEDMVVFRLDQNGNRLWSTYYGGTGDEQGKGI
                     ASDAGGNCYVTGSTGSNDFPTLNPVQPGYGGGVHDVAMLKLNGAGVRQWATWLGGLQS
                     DNGTAITCDVNGNCFVTGYTNSSDFPVTPGAAQTVKNIGYDAFVFKMNSTGVVPWATF
                     YGGDDDDKGRAIALDPGMTDLYFTGSTLAGGFPVTAGVFQNSSMSAYNAEDAFIVKMD
                     TSQSVQFASYCGGSDADFGQGIAVDLSGNIFITGYTLSANFPVVNPGGGAYVDSTIGS
                     LGTHDAFVVECNSSGTQRLWSTYFGGSSPDMSFAIAYEAYAGIYICGNTASTDFPVQM
                     PSDNLFYQPTQGDGGNFNDKFIAWFGQNDSLEWATYYGGANDDEAFGVCTDANDNIFM
                     AGLSNNDIDVQKFAPGFPLAVFPGNETQNDLSVYPNPAQQYISVTVNSQANENPEFQL
                     MDLSGKIIRQAEAGPAGPGNV"
     gene            2356..2841
                     /locus_tag="VFU15_10950"
     CDS             2356..2841
                     /locus_tag="VFU15_10950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010662030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HEU4718345.1"
                     /translation="MDLNEFRIDASSLQKANEKFFAKLKRTKPKDLDANVHRFHEEAF
                     EHIDCLQCANCCKTTSPIFYQKDIGRVAQHLRMKPGAFIEKYLHIDEDGDYVLNSAPC
                     PFLDAENYCLVYESRPNACREYPHTDRKKMHQVLDLTLKNTMVCPAVLEIAEKLRKIY
                     L"
     gene            complement(2865..3713)
                     /locus_tag="VFU15_10955"
     CDS             complement(2865..3713)
                     /locus_tag="VFU15_10955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718346.1"
                     /translation="MRFLIAALIFFALSCGNNNVPEQEHVFVLSGKKSLVPDSAKQRL
                     LRILEGGWVNEDYISALDDLHSPVKAASAGLPERQMAFDISHVNGDTLVNGRGRMDYD
                     ATERFDIVFFMKRDSTVGFRIGENPGYNDEQYALNYSFENNDTILLLTVSGEKTVTYR
                     FRRVFRAFPESDNVTITAIEYFINETLFNGKWKSSDGKETEFLPDGRVKNFKGYKRYS
                     VYCEKENAVSRPDEISFYNDTSGVTYAYTLIDNVLKFYELKESDDGMEISRGKLVTSM
                     EMVKAE"
     gene            3812..6601
                     /locus_tag="VFU15_10960"
     CDS             3812..6601
                     /locus_tag="VFU15_10960"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEU4718347.1"
                     /translation="MKKICFPAIFICLALISACSHPEKKKIIPPGDPMEDDAEARNAW
                     EFKRLAGPDGTIPDHMREKELAYSATLPNDRSVTYGSSNERTAQQATWSERGPWNVGG
                     RTRALGIDKLNENHLIAGTTCGGIWNSNDGGSTWTEVTPPGVYHGITCLEQDKRPGHE
                     STWYAGSGEAYGQSASGGSAYYLGNGMLKSTDNGATWTPITVTVSNTPQSFDTFWDMN
                     WDLALDGTDTANDIVYTACLGAVYKSVNGGTSWTPVRGGNLSNYSYFTDVAVTKDSGI
                     VYATMSSDGPDHGIWRSADKGATWSKISTAGFGDTACNRVVMGFNPMNEHEIYFLGNT
                     NGIGMPDTNFQGDIEWNVLWRYTYLGGDGTGSGGMWEDLSANLPSAEHFGQFGDFNAQ
                     GSYDLVVRVKPDDPNTVFIGGTNIFRNTNRFNDTASTSYIGGYLPYSALPVIASYPDH
                     HPDQHMMTFLPSDPNVMFSTNDGGIFKTTDCTQSTVSWTSLDNGYITSMFYTVAVDHA
                     TPGNDIVIGGAQDNGSWWTNTTNLTTPWVSPRSGDGSYCAIADNQSAYYFSIQNGKMM
                     RALLDGSGNVTSFARIDPIGGKGYQFINPFVIDPNNNNIMYLAGGKYLWRNDNLAGIP
                     MAGNWDSISTNWVKWNDSVPVANVNITALAVSKTPANRVYYGTELKRVYRVDNANTGT
                     PTPVDITPTSFPSSGFVSCVAVNPNDADQVMVVFSNYQVYSLFYSADGGTTWTKVAGN
                     LEQVVSGGGNGPSLRWASFLPVSDGMIYLVSSSTGIYATDTLNGTATVWTRQGDQTIG
                     TIVCDMTDVRISDGLVVLATHAHGIFSTHLTSVNDIVSVHENATISSAGINVYPNPAG
                     NELTIAYTTDKDGEVQIQLLDELGRVIRNLRDEKQSPGNYSVHADVSGLAAGMYYVRM
                     TTGTSVNVKPLVVQH"
     gene            6774..7208
                     /locus_tag="VFU15_10965"
     CDS             6774..7208
                     /locus_tag="VFU15_10965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718348.1"
                     /translation="MKTKHLPERTIALWFDHHIAHFVIPSHDQWMIESVESDYERKER
                     EPGQKDDVADFGRNRISNNEYGKHNRLRNELKDYYKSIEDRLVKAENILLFGPGTAKD
                     ELFNLLMENKSFDGKRISVESADKMTENQLIAFADNYFTAKA"
     gene            complement(7233..>7880)
                     /locus_tag="VFU15_10970"
     CDS             complement(7233..>7880)
                     /locus_tag="VFU15_10970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718349.1"
                     /translation="KTTEMMKRSGFVLLALLLLQVEMHSQAAVKSFRGLSRPEKCWVA
                     AHPFIAKKAFRCSVEARAVSDSIGKTGTLKDADGGQLDAFRHTYWMALLVQEISPRKA
                     RKLGEAHEKGNYLGFKKGKQEDNSRADSMACVMDLRNNETGIQLGTAFGNDTSATKKS
                     LVDSVVASVKKGNAVILSKDANGNYLDCEGNRIDLTLYEKKWYVPKCLVKSDQLK"
BASE COUNT         1889 a         2195 c         2016 g         1780 t
ORIGIN      
        1 aacatttccc ggtccggccg gtccagcttc cgcctggcgg attattttcc ctgacaaatc
       61 catcagctga aactccgggt tttcattcgc ctgtgaattt accgttacgc tgatgtattg
      121 ctgcgccggg ttgggataaa cagaaagatc gttctgcgtt tcattgccgg gaaaaacggc
      181 cagcggaaat ccgggtgcga acttctgaac gtcgatgtcg ttgttcgaca ggccggccat
      241 gaaaatattg tcgttcgcat ccgtgcatac gccgaacgct tcgtcatcat tcgcgccgcc
      301 gtaatacgtc gcccactcga gcgaatcgtt ctgcccgaac caggcgatga atttatcatt
      361 gaagtttccg ccgtcgccct gcgtgggctg gtagaaaaga ttgtcggacg gcatctgcac
      421 gggaaaatcc gtcgatgccg tatttccgca gatataaatt cctgcgtaag cttcgtacgc
      481 gatcgcgaac gacatgtccg gcgagcttcc cccgaaatac gtcgaccata aacgctgcgt
      541 gcccgagctg ttgcattcca cgacaaacgc gtcgtgcgtg ccgagactgc cgatggtgct
      601 gtcgacataa gcgccgccgc cgggattcac caccgggaaa ttcgccgaaa gcgtgtagcc
      661 cgtgatgaaa atatttccgc tgagatcgac cgcgatccct tgtccgaaat cggcatccga
      721 accgccgcaa tacgaagcga actgcacgga ctgcgacgta tccattttca cgatgaatgc
      781 gtcctccgca ttgtaggcgc tcatgctgga gttctggaaa acgcccgccg taacgggaaa
      841 tccgccggca agcgtggagc cggtaaaata aagatccgtc atccccggat cgagcgcgat
      901 cgcacgtcct ttatcatcgt catcgccgcc gtaaaatgtt gcccagggaa caacaccggt
      961 gctgttcatt ttgaaaacga acgcgtcgta accgatattt tttacggtct gcgcggctcc
     1021 cggcgtcacc gggaaatcgc ttgaattcgt atagcccgtg acgaagcaat tcccgttcac
     1081 atcgcaggtg atcgctgttc cgttatcgct ctgcagtccg ccgagccacg tggcccactg
     1141 gcgtacgccc gcgccattca gcttcagcat cgccacgtca tgcacaccac cgccgtagcc
     1201 gggctgcacc gggttcagcg tgggaaaatc gttcgagcct gtacttccgg tcacgtaaca
     1261 attccccccc gcgtcagacg caattccttt tccctgctca tcgccggttc ctccgtagta
     1321 ggtcgaccac aagcggttcc cgttctggtc gaggcgaaaa acgaccatgt cttcaaaacc
     1381 gtacagctgt ccctggtaag cattctgcac agggaaatcc gttccgtctg aagcgcccgt
     1441 cacgatcaca tcaccacttc cgtcaccggc tgcgatagtt gcataaccgt aatctgcgct
     1501 ggtgctcgcc tgcgtggacg accactgcaa gggcggatcg ataacgagtg tttcattcac
     1561 ggaataggat gagattgcgt acgaaacttc cgcatgggcg gcagacgatt tccgcatgca
     1621 cccgggcaga tcgaggagaa tatcaaaaga tgccgggaca acacggccgc tgccctgttc
     1681 ataaacatgc agctttcctt cattgagcga accataagcc gtcggaaaaa caagatcggc
     1741 atccgaagct ttcatgtcgc cgaggttttc accggtgtaa ctgatccgga tcaacgccgg
     1801 gtcagcaccg ggatgaacga tgaaatcatg tgaaactccc ttttcattat cggcagtaag
     1861 cacccagtcg atgcccgggt aaatatttct gatcgtcacc ttgtgccacg atcttacgcc
     1921 ggtaattccc tgcggacaat ccgcgaggta ataattcgtg acgccgggaa gcgggtcttc
     1981 cgttgcgata ttttctttcc ggatgtccgc cccggtgagc gtcatcccga tccgcgacca
     2041 accggtcact gaaacggaat tatcgtccga cgttttttcc tttttcgtaa aaaaataatc
     2101 gatcccgtgg tccgtcacgt acatcgcggc ggaggtcccg tacgagcgga aaagcacgtt
     2161 tgccggataa tctttggttc cggccgtccg gaactggcct ttgttttccg tgaaaccaat
     2221 atcagtggca aaagcggaaa ccgtgaaaga ggcgaacagg aaagagagta gaattttatt
     2281 catagcggca ggatttaaga ttgtaattta cgaaaagaac agctcctttt catctccgcc
     2341 gggtaacttt gcagaatgga tctgaacgaa ttccgtattg acgcttcatc attgcaaaag
     2401 gccaacgaaa aattcttcgc gaagctgaag cgcacgaagc cgaaagacct cgacgcgaat
     2461 gtgcaccgtt tccacgaaga ggcgtttgag cacatcgact gcctgcaatg cgcgaactgc
     2521 tgcaaaacga catctcccat tttttaccag aaagacatcg ggcgcgtggc gcagcacctg
     2581 cgcatgaaac cgggtgcgtt cattgagaaa tacctgcata tcgacgagga cggcgattat
     2641 gttctgaatt ccgcaccctg cccgttcctc gatgcggaaa attattgcct cgtttatgaa
     2701 tcgcgtccca acgcctgccg cgagtacccg cacacagacc ggaaaaaaat gcaccaggtg
     2761 ctcgatctta cgttgaaaaa cacaatggtc tgcccggccg tgctggaaat agcggagaag
     2821 ctgcgcaaaa tatacctgta gtgcagttcc acattcaaca tttatcattc agccttcacc
     2881 atttccatcg aagtcaccag cttccctctc gagatctcca tgccgtcatc cgattctttc
     2941 agctcataaa atttcagcac gttatcgatc agggtgtagg catacgtcac gcctgaagtg
     3001 tcgttgtaaa aagagatctc atccgggcgc gaaactgcat tttccttttc gcagtaaacc
     3061 gaataacgtt tatatccttt gaaatttttc acacgcccgt cgggcagaaa ttcggtttcc
     3121 ttcccatcgc tgcttttcca tttcccgttg aaaagcgttt cattgatgaa atattcgata
     3181 gcagtaatgg taacattatc gctttccggg aaagcccgga acacgcgccg gaagcggtag
     3241 gttactgttt tttcaccgga aacggtcagc agcaggatag tatcgttgtt ctcgaacgaa
     3301 taattcagcg catactgctc atcgttgtaa ccgggatttt ccccgatgcg aaagcccacc
     3361 gtgctgtccc gcttcataaa aaatacgatg tcgaaacgct cggtcgcatc gtaatccatt
     3421 cgtccgcgcc cgttgacgag cgtgtcgccg ttcacatgcg aaatatcgaa cgccatctgc
     3481 cgctcgggaa gtccggccga agccgctttc acgggcgagt gaagatcatc cagcgcggaa
     3541 atgtaatctt cattcaccca tccgccttcg agtatgcgca ggagacgctg cttcgcagaa
     3601 tcgggaacca ggcttttctt tccggaaaga acaaacacat gctcctgttc cgggacattg
     3661 ttatttccac atgaaagcgc aaagaagatc aacgcagcaa ttaaaaaccg catggcgcta
     3721 aattaaggaa acacactccc ctgcccttcg cctgtcggct gcttttccta aatttgattt
     3781 ccacacaaac cattgcccaa aatttgctgc catgaaaaaa atctgctttc ccgcgatctt
     3841 tatttgcctc gcactgatct ccgcctgttc ccacccggaa aagaaaaaaa ttattcctcc
     3901 cggcgatcct atggaggatg acgcggaagc aaggaacgcc tgggaattca aacgccttgc
     3961 gggtcctgac ggaaccattc ccgatcacat gcgcgaaaaa gagctcgcct attccgcgac
     4021 gcttcccaat gaccgttccg ttacctacgg cagcagcaac gaacgtactg cgcagcaggc
     4081 cacctggtcg gagcgcggtc cgtggaacgt gggcggaaga acacgcgcgc tcggcatcga
     4141 caagctgaat gaaaatcacc tcatcgcagg aaccacctgc ggcggaatat ggaacagcaa
     4201 cgacggtggc agcacatgga cggaagtcac gccgcccggt gtttaccacg gcatcacctg
     4261 cctcgagcag gacaagcgcc cgggccacga aagcacatgg tacgcaggtt cgggagaagc
     4321 gtacggccaa tctgccagcg gaggcagcgc ctattacctc ggcaacggca tgctgaaaag
     4381 cacggacaac ggcgcaacat ggacgcccat cacggtaact gtttccaaca cgccgcaatc
     4441 gttcgacaca ttctgggaca tgaactggga tctcgcgctt gacggaacgg acacggcgaa
     4501 cgatatcgtt tatacggcgt gtctcggtgc ggtgtacaaa agcgtgaacg gcggcacaag
     4561 ctggacgccg gtgcgcggcg gcaacctcag caattattcc tacttcaccg atgtcgcagt
     4621 gacaaaagat tccggcatcg tttacgcgac gatgagttcc gacggacctg atcacggcat
     4681 ctggcgcagc gccgacaagg gtgcgacatg gtcgaaaatt tccaccgcag gttttggcga
     4741 tacggcgtgc aaccgtgtgg tgatgggttt caacccgatg aacgagcatg aaatttattt
     4801 tctcggcaac acgaacggga tcggcatgcc cgacacgaat ttccagggcg atatcgaatg
     4861 gaacgtgttg tggcgctaca cttatctcgg cggcgacgga acgggcagcg ggggaatgtg
     4921 ggaagatctt tccgcgaatc ttccttcggc ggagcatttc ggtcagttcg gcgatttcaa
     4981 cgcgcagggt tcctacgatc ttgtcgtgcg tgtgaagcct gatgatccga acactgtttt
     5041 catcggcgga acgaatattt tccgcaacac gaaccgcttc aacgatactg cgagcacgag
     5101 ctacatcggc ggttatcttc cttactccgc actgcccgtt atcgccagct atcccgatca
     5161 ccatcctgat cagcacatga tgacgttcct gccttcggac ccgaacgtga tgttcagcac
     5221 gaacgacggc ggaattttca aaacgaccga ctgtacgcag agcaccgtct catggacttc
     5281 gctcgacaac ggctacatca cgtcgatgtt ttacacggtc gcggtcgacc acgccacacc
     5341 cgggaacgac atcgtcatcg gcggggcgca ggacaacgga agctggtgga cgaacacaac
     5401 caatcttacg acgccatggg tttctccgcg cagcggcgac ggttcgtatt gcgcgatcgc
     5461 cgataaccag agcgcgtatt atttctcgat acagaacgga aaaatgatgc gggcgcttct
     5521 cgacggaagc ggcaacgtca cttctttcgc acgcatagat cccatcggcg gaaaaggtta
     5581 ccagttcatc aacccgttcg tgatcgatcc gaacaacaac aacatcatgt atctcgccgg
     5641 tggaaaatat ttgtggcgaa atgataacct cgcgggaatt cccatggccg gcaactggga
     5701 ttccatcagc acgaactggg tgaagtggaa cgattccgtt cctgtggcga acgtgaacat
     5761 caccgctctc gcagtcagca aaactcccgc gaaccgcgtg tattacggaa cagagctgaa
     5821 gcgcgtgtac cgcgtggata acgcgaacac cggcacgcct acaccggtcg acataacgcc
     5881 gacgtccttc ccctcatccg gttttgtgag ctgcgtggct gtcaatccca atgatgccga
     5941 ccaggtaatg gtggtgtttt caaactacca ggtgtacagc ttgttttatt cagcagacgg
     6001 tggaaccacg tggacgaaag ttgcgggtaa cctcgaacag gtggtctcgg gcggcggtaa
     6061 cgggccatcg ctgcggtggg cgtcgttcct gccggtgagc gacggcatga tctatctcgt
     6121 ttcatcgagc acgggcattt acgccaccga cacgctgaac ggaaccgcaa ccgtatggac
     6181 acgccagggc gatcaaacta tcggcacgat cgtgtgcgac atgaccgatg tgcgtatttc
     6241 cgacgggctt gttgtacttg ccacgcacgc gcatggaatt ttttccacgc acctcacgag
     6301 cgtgaatgat atcgtcagcg tgcatgaaaa tgcgacgatc tcttccgccg gcataaacgt
     6361 ttacccgaac ccggcaggca atgagctgac gatcgcttac actacggaca aagatggcga
     6421 agtgcagatc cagttgctcg atgagctggg ccgcgtgatc cgcaacctgc gcgacgagaa
     6481 gcaatcgccg ggaaattatt ccgtgcatgc ggatgtttcc ggcctggcgg caggaatgta
     6541 ttatgtgcgt atgaccacgg gaacgtccgt gaatgtgaaa ccactggtgg tacagcatta
     6601 atcggtgcat tttacgtagc ggtactgcct gcacttgtgc tgtgggcttt gggggcttcg
     6661 cggttccttc attttgtgta atgctatagc gttactttcc gggacaacaa agatcacggc
     6721 ccattctgat tttcctcatg cacccgccgg gcggcacgga atatttttac ttcatgaaaa
     6781 caaaacacct gcccgaaaga acaattgcgt tgtggttcga ccatcacatt gcgcattttg
     6841 tcattccttc tcatgatcag tggatgatcg aatccgttga atcggattat gaacggaaag
     6901 aacgcgagcc cggccagaaa gacgatgtcg ccgatttcgg acgaaatcgt atttcgaaca
     6961 atgaatacgg gaagcataac cgtctccgga atgagctgaa ggattactac aagtcgatcg
     7021 aagaccgcct ggtgaaagca gaaaacattc ttcttttcgg tccgggaacg gcgaaagacg
     7081 aactgttcaa cctgctgatg gaaaataaat ccttcgacgg aaaacgtatc agtgtggaaa
     7141 gcgcagataa aatgacggag aaccagctga tcgctttcgc agataattat ttcacagcga
     7201 aagcttgacg aggaattgcg gacttatccg ggttatttta gctggtccga ctttacgagg
     7261 catttgggaa cgtaccattt cttctcgtaa agcgtgagat caatgcggtt cccttcacag
     7321 tcgaggtaat ttccgttcgc gtctttcgaa aggataaccg cattcccttt ttttaccgag
     7381 gcaacaaccg aatccaccag tgatttcttc gtcgctgatg tgtcatttcc gaatgctgtg
     7441 cccagctgaa ttcccgtttc gttgttgcgg agatccatca cacacgccat cgagtcggcg
     7501 cgggaattat cttcctgttt tcctttttta aagccgaggt aattcccttt ttcatgcgct
     7561 tcaccgagct tcctcgcttt ccgcggggaa atttcctgca cgagcaacgc catccagtag
     7621 gtgtgccgga acgcgtcgag ctgtccgcca tccgcatctt tcagcgtgcc ggttttgcca
     7681 atggaatccg aaacggccct tgcttcaacg gaacagcgga aggccttttt cgcaatgaac
     7741 ggatgcgcag ccacccagca tttttcggga cgggaaagtc cgcggaacga tttcaccgca
     7801 gcctgcgaat gcatttccac ctgtaagagt aaaagcgcca gcaggacgaa gccgcttctt
     7861 ttcatcatct cagtagtttt
//
LOCUS       DASTSF010000384         2847 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1591997,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000384 DASTSF010000000
VERSION     DASTSF010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2847
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1591997"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            352..1482
                     /locus_tag="VFU15_10975"
     CDS             352..1482
                     /locus_tag="VFU15_10975"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEU4718350.1"
                     /translation="MVIVVNTRLLIHGKTDGIGRFAYESLIRITRSHPGDQFVFLFDR
                     NIHEDFIFSENITPVKLLPPARRPWLWNLWFNVSVKGVLRDLQPDLFLSPDGFLPLNT
                     KTKCLPVIHDLNFEHYPQDMPASYSKWYRKKFPLFAKQAARIATVSEFSKNDIATRYG
                     ISPGKIDVVYNGASAGFVPLGEERKKEVRKKYTNEAPYFLYIGALHPRKNLERLVRAF
                     GAFRKNSETKTKLVITGGNYWKYDSLQKAVDESGAKEEIVFTGRLGENELHAVTGAAR
                     ALAYVPYFEGFGIPIVEAMACDVPVLAANVSCMPEVGGDAVLYADPLSENSIADALAR
                     LDKDEALRNSLVEKAKVQRQKFSWDRTASLLWDSIEKTVNAR"
     gene            1479..2081
                     /locus_tag="VFU15_10980"
     CDS             1479..2081
                     /locus_tag="VFU15_10980"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007560069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEU4718351.1"
                     /translation="MSLRGFLHEKLAEAGCDEAGRGCLAGPVFAAAVILPRDFRHPLL
                     NDSKQLSDKQRKELRPLIEEKAIAWAVACVSVEEIDEINILNASFEAMHRAVKKLSAV
                     PQHLLIDGNRFTPFEGIKHTCIIGGDAKFKSIAAASILAKTYRDDLMDALHEKFPQYG
                     WIKNKGYGTKAHRDAIQKFGATEHHRKSFRLLPDQLKIEF"
     gene            2137..>2847
                     /locus_tag="VFU15_10985"
     CDS             2137..>2847
                     /locus_tag="VFU15_10985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718352.1"
                     /translation="MKKNYLAALCGAAAFLFPVLAGAQCTLCGTGADSIYEATSNVQL
                     PGAVYNFTSFTIDTGVTVTVTGPQPLVIYCSGAVNIQGTITVKGDDGSPGITSSMAGA
                     GGLGVAGGQQGGDGVYSASVGPLDGVAGSGAGSGGAGMQWSGGGGGGYSTYGDTAGTN
                     GGFGGSPYGNAQINPYDAGSGGGGGSGGYACGGGGGGAGGGYISISSCNGIIIGASGM
                     IDARGGNGGSDGTGNCGGG"
BASE COUNT          632 a          806 c          789 g          620 t
ORIGIN      
        1 gaacagcgtt gaccgtttcc tgttttcctc cgtgcacttt gtgacaagct tcgtgtactt
       61 cgtggttttg atttacaacc tttcaacttt ccaactttga aactgaattg tcttcgtttc
      121 ccttcgtgcg ctttgtgaaa atgctttgtg ctcttcgtgg ttaaaacggt tattagttcc
      181 aggtttcgga ctgtccgggc ctgaacagcg ttgaccgttg ttctctgtgt ttcctctgtg
      241 aaacggcctc cgtggttctc tgtgttaaaa agaacttttc agtttcgggc cctgcatttg
      301 ccgtctggca tttgaatcgt ttcctcccat ccaaactcct accttcgcgg catggtcatc
      361 gtcgtcaaca cgcgcctcct cattcacgga aaaacagacg gcatcgggcg gtttgcctac
      421 gaatcgctga tccgcattac gcgatcgcat cccggagacc agttcgtttt ccttttcgac
      481 cggaacatcc acgaagactt cattttttcg gaaaacatca cacccgtgaa gcttcttccg
      541 cccgcaagac gtccgtggct ctggaacctc tggttcaatg tttccgtgaa aggcgtcctg
      601 cgcgatctgc agcccgatct ttttctttcc cctgacggtt ttcttccgct gaacacgaaa
      661 acgaaatgcc tgcccgtcat ccacgacctg aattttgagc attacccgca ggacatgccc
      721 gcgtcgtatt cgaaatggta ccgcaaaaaa ttccccctgt tcgcgaagca ggcggcgcgc
      781 atcgccacgg tttccgaatt ttcgaagaac gatatcgcaa cccgctacgg aatttccccg
      841 ggaaaaatcg acgtcgttta caacggcgct tcagccggtt ttgttccgct cggtgaagag
      901 cgaaaaaaag aagtgcggaa aaagtacacg aatgaggcac cgtattttct ttacatcggt
      961 gcgctgcacc cgcggaaaaa tctcgaacgc ctcgtgcgcg cgttcggggc gttccggaaa
     1021 aacagtgaaa caaaaacaaa gctcgtcatc accggcggga attactggaa atacgattcg
     1081 ctgcagaaag ccgtggacga aagcggcgca aaagaagaga tcgtcttcac cggccgcctc
     1141 ggtgaaaatg aattgcatgc agtgaccggc gctgcgcgtg cgctggcgta tgtcccgtat
     1201 ttcgaaggtt tcggcatacc gatcgtggaa gcgatggcgt gcgatgttcc cgtgctggcg
     1261 gcgaatgttt cctgcatgcc ggaagtgggc ggtgatgccg tgttatacgc cgacccgctc
     1321 tcggaaaaca gcatcgcgga tgcgctggct cgtctcgaca aagacgaagc gctgcggaat
     1381 tccctcgttg aaaaagcgaa agtgcagcgg cagaaatttt cctgggacag gaccgcgtcg
     1441 ctcctctggg attccatcga aaaaacagtg aacgccagat gagcctgcgc ggctttctcc
     1501 acgaaaaact cgctgaagcg ggctgcgatg aagccgggcg cggctgcctc gccggacctg
     1561 tcttcgctgc ggccgtcatt cttccccgcg atttccgtca cccgctgctc aacgattcga
     1621 aacagctttc cgacaagcag cggaaagagc tgcgcccgct gatcgaagaa aaagccatcg
     1681 cctgggcggt ggcgtgtgta agcgtggagg agatcgacga gatcaacatc ctcaacgcat
     1741 cattcgaagc gatgcaccgc gccgtgaaaa aactttccgc cgttccgcag cacctgctca
     1801 tcgacgggaa ccgtttcacg ccgttcgaag gaataaagca cacctgcatc atcggcggcg
     1861 atgcgaaatt caaatccatt gcggccgcat ctattcttgc gaaaacctac cgcgacgatc
     1921 tcatggacgc gctgcatgaa aaatttccgc agtacggctg gataaaaaac aaaggctacg
     1981 gcaccaaagc acaccgcgac gccatacaaa agttcggtgc gaccgagcat caccggaaaa
     2041 gtttccgcct gcttccggac cagctgaaga tcgaattctg aaattcctgt tcacgggaaa
     2101 tttttctaaa ttgtaacgat aaccaaaatc cgctctatga aaaaaaacta cctggcagcg
     2161 ctttgcggcg cagcagcctt cttattcccg gttctcgcag gcgcgcaatg cacgctgtgc
     2221 ggaacgggcg ccgacagtat ttatgaagcc acgtcgaacg tgcagcttcc cggcgccgtt
     2281 tacaatttca catcattcac catcgacacg ggcgtaacgg tgactgttac cggtccgcag
     2341 ccgctcgtta tttactgctc gggtgctgtg aatatccagg gcacgatcac cgtaaaaggc
     2401 gatgacggca gccccggaat aacaagcagc atggcgggtg ccggcggact cggcgtcgca
     2461 ggcggacagc agggcggcga tggggtgtat tctgccagcg ttggcccgct tgacggcgtt
     2521 gcaggaagcg gcgcaggttc gggcggcgca ggcatgcagt ggagcggcgg tggcggcggc
     2581 ggatacagca cctatggtga caccgccggc acgaatggcg gattcggcgg atctccttac
     2641 ggaaacgcgc agatcaaccc gtatgacgcg ggctcaggcg gcggcggcgg ttccggcgga
     2701 tatgcatgtg gcggcggcgg cggcggagca ggcggcgggt acatttccat ttcttcctgc
     2761 aatggcataa tcatcggcgc aagcggcatg atcgatgcgc gcggcggcaa cggcggttcc
     2821 gacggaacag gtaactgcgg cggcggc
//
LOCUS       DASTSF010000385         2919 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_198176,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000385 DASTSF010000000
VERSION     DASTSF010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2919
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198176"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1288)
                     /locus_tag="VFU15_10990"
     CDS             complement(<1..1288)
                     /locus_tag="VFU15_10990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718353.1"
                     /translation="MNKKYLIALFALSFFAVRCFAQTNVDWDEKNFPDRKAYKEAKKH
                     FDNGNDYFKQGQDIQSSELSRFIDKNHYLPNGINELGGAGSEAFHLALDEYLQAKTVN
                     PNNALMNFRIGVCYYNNSAQKLMGLTYLELAEKLNPSVDPMLQYYLGREYHLSAKWDD
                     AITAYRSYLATLNGDPKNNRDKIADMNRKIQECINGKEFQKNPQRVFIDNIGPTVNTS
                     DPEYTPIITADESTMLFTSRRSTSTGGQKDPDNQYYEDLYRTDYVNGQWSPPVNMGKP
                     VNTDDHDATAGISPDGQKLYIYRPTHGGDIYESILKGDKWTDPERMNKNINSDGHESS
                     VSLSYDGKKLYFISDKDGGLGNRDIWVSTMDAKGKWGESANIGAPINTMYGEEGVFVH
                     PDGKTIYFSSQGHNSMGGYDIFKSEFKDGKWGEPENL"
     gene            complement(1311..2357)
                     /locus_tag="VFU15_10995"
     CDS             complement(1311..2357)
                     /locus_tag="VFU15_10995"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU4718354.1"
                     /translation="MKKLLLALGLFMAFSRISFAQDTVKASTLPGAPEAETYYNSGVT
                     KFNARQYTDALSDFNKAIELKADFEKAYFQRGQTRVMLVMNTGAIEDFTKCIDLNPSA
                     DYAYFARGMVKESDGDLAGAADDYAKTISLNPNHDKAFYHSGDIKFQNKDYKNAITDF
                     DKAIQINAGDAYYWNDRGSARRESGDLKGAIADYQQAIKVNPGLPFVYNNLGSAKRES
                     GDLKGAIESYTTAVNMKTDYYIAYNNRGYAKYLDGDYDGAMVDFNQAIKLKNDYAYAY
                     VNRAAVRYKQRDYAGAVKDCDEAIRLNANYGEAYLNRGIAKEMLRDNSACDDFKKAAN
                     LGIAAAKQYSNDCN"
     gene            2505..>2919
                     /locus_tag="VFU15_11000"
     CDS             2505..>2919
                     /locus_tag="VFU15_11000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008244534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family peptidase"
                     /protein_id="HEU4718355.1"
                     /translation="MAKTKSAAKKSSLLGKDEIAFLERYLNNPSPTGFESPGQKLWLD
                     YIRPYIDDYFVDTYGTAVGIVNPGAPYKVVIEAHADEISWFVHYITKDGFIYLRRNGG
                     SDHLIAPSKRVNIHTENGIVKAVFGWPAIHVREPGK"
BASE COUNT          619 a          740 c          754 g          806 t
ORIGIN      
        1 cgaggttctc gggctcaccc cattttccat ccttgaattc ggatttgaaa atatcgtagc
       61 cgcccatgga attgtgtccc tgcgagctga aatagatcgt cttcccgtcg ggatggacga
      121 acacaccttc ttcaccgtac atcgtgttga tcggtgcgcc gatgttggct gattcgcccc
      181 attttccttt ggcatccatc gtcgacaccc agatgtcccg gttgccaaga ccgccgtcct
      241 tgtcactgat gaagtacagt tttttcccgt catacgaaag cgatacggat gattcgtgcc
      301 cgtcggaatt gatgttcttg ttcatgcgtt cggggtccgt ccacttgtcg cctttcagaa
      361 tgctttcata aatatctccg ccgtgcgtcg ggcggtagat gtacagcttc tgtccgtcgg
      421 gagaaattcc ggccgttgca tcgtggtcat ccgtattgac cggcttgccc atgttcaccg
      481 ggggcgacca ctgcccgttg acgtaatccg tgcgataaag atcttcgtaa tactggttat
      541 ccgggtcttt ctgtccgccc gttgaagtgg aacggcgcga cgtgaaaagc atggttgatt
      601 catcggcggt gataatgggc gtgtactccg gatcgctggt gttgaccgtg ggcccgatgt
      661 tgtcgatgaa aacgcgctgc ggatttttct ggaattcctt tccattgatg cactcctgta
      721 tcttacggtt catatccgcg atcttgtcac ggttgttttt cggatcgccg ttcagcgtgg
      781 cgaggtaaga acggtacgca gtgatcgcat catcccactt cgcgctgaga tgatattccc
      841 ggcccaggta atattgcagc atcgggtcga cagaaggatt cagcttctcg gccagctcga
      901 ggtacgtgag ccccatcagc ttctgcgcgg aattattgta atagcagacg ccgatcctga
      961 aattcatcag cgcattgttc gggttcactg ttttcgcctg caggtattca tccagcgcga
     1021 ggtgaaatgc ttccgacccg gctccgccca gctcgttgat gccattcggc aggtaatgat
     1081 tcttatcgat aaaccggctc agctcggacg actggatatc ctgtccctgc ttgaaataat
     1141 cattgccgtt atcgaaatgc ttctttgctt ccttgtaggc tttacggtcc ggaaaattct
     1201 tctcgtccca atcaacgttg gtctgggcaa aacagcgcac tgcgaaaaaa gaaagggcga
     1261 aaagcgcgat aagatatttt ttattcatgg gagtgtaggc gattgaaaga tcagttgcag
     1321 tcgttggaat attgtttggc ggccgcaata ccgaggttgg cggctttctt aaaatcgtcg
     1381 caggcggaat tatcgcgcag catttctttc gcgatgccgc ggttgaggta ggcttccccg
     1441 taattcgcat taaggcggat cgcttcgtcg caatctttca cggcgccggc ataatcgcgc
     1501 tgcttgtaac gcacagccgc gcggttgaca tacgcatacg cgtaatcatt cttgagcttt
     1561 atcgcctggt tgaaatcgac catggccccg tcgtaatccc cgtcgaggta tttggcataa
     1621 ccgcggttat tatatgcgat gtaataatcc gttttcatgt tcactgccgt cgtatacgac
     1681 tcgatcgcac ctttgagatc accgctctcc cgttttgcgc tgccgagatt attatagacg
     1741 aacggaagac cgggatttac cttgatcgcc tgctggtaat cggcaatagc gcctttgagg
     1801 tcgccggatt cccgcctggc gcttccgcgg tcgttccagt aatacgcatc gcctgcgtta
     1861 atctggatgg ctttgtcaaa atcggtgatc gcatttttat aatccttatt ctggaacttg
     1921 atatcgccgg aatgatagaa cgctttatcg tggttcggat tgagcgaaat ggttttggca
     1981 taatcatctg cagcgccggc caggtccccg tcgctttcct tcaccattcc gcgggcgaag
     2041 taagcgtaat cggccgaggg attaaggtcg atgcatttcg taaaatcctc gatagccccg
     2101 gtattcatca ccagcataac gcgcgtttgt ccgcgctgga aatatgcttt ttcaaaatcc
     2161 gctttgagct cgatggcttt gttgaaatcc gaaagtgcat cggtgtattg cctggcgtta
     2221 aacttggtaa cgccgctgtt gtaatacgtt tctgcttccg gggcgccggg cagcgtggac
     2281 gctttgacgg tatcctgggc gaatgaaata cggctgaaag ccatgaaaag gcccagggcg
     2341 agtaacagtt ttttcattgg tgtacggtgt tgtccgtcta aaataacaaa attcaggccg
     2401 atgttccgat ctttttacgg gctcctccgg atattttatt ctaatttttt ccgtttgggt
     2461 gagacgcggc ttaataagta tctttaaggt ccaattgagc atttatggca aaaaccaaaa
     2521 gcgctgcgaa aaagtcttct ctattgggta aagacgaaat cgctttcctc gaacgttacc
     2581 tcaacaatcc ttcacccacc gggtttgaat cgcccgggca gaaactgtgg ctcgattaca
     2641 tccggcccta tatcgatgat tatttcgtcg atacctatgg aacggcggtc ggcattgtga
     2701 atcccggtgc cccgtacaaa gtcgtcatcg aagcgcacgc ggatgaaatt tcgtggttcg
     2761 ttcattacat caccaaagac gggtttattt acctgcgaag gaacggcggt tccgatcatc
     2821 tcatcgctcc ttccaagcgg gtgaacattc acacggaaaa cggcatcgtg aaagccgtgt
     2881 tcgggtggcc ggcgattcat gtgcgcgaac ccgggaagg
//
LOCUS       DASTSF010000386         6647 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_752069,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000386 DASTSF010000000
VERSION     DASTSF010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 6647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6647
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_752069"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1596)
                     /locus_tag="VFU15_11005"
     CDS             complement(<1..1596)
                     /locus_tag="VFU15_11005"
                     /inference="COORDINATES: protein motif:HMM:NF025627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004062 - aryl sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aryl-sulfate sulfotransferase"
                     /protein_id="HEU4718356.1"
                     /translation="MKRTAALLFFFVALNAFAQVTPFSLSKVVIREPFIPSSPILPVP
                     GSKLNYTQVMFEHPVTEKAVYYVVNVFSVNGKDTVPFVSVTDSTNATMISGFGFGKEY
                     AWNYVSTGAKKKKIATSPWYGFSILPLPEGSRIRVVKNDSAKTQGGLVSFDYAEVITG
                     RDGKPVWFLPSSGKGDFVRDDEVRDLRISKAGTFTFITEKNAFEVSVDGRTMWKAPQN
                     PRDTSQHYHHGFERLPNGNFMVMGNHAVMRKVPGDSTYVRIVFGTVEEFDRSGKMVWK
                     WEAENYFTDADIFSRKTPDGKPDVSTHMNAFSVDDSMHFVYAGFRDMSRIVKVDYRTG
                     KAVQAYGKRMPSGEAKYANGFYWHQHDASIQPDGTIAVFDNDSIADPGISSRVLIFSQ
                     PASPGDSCRIVWSFPCRFDSLSDGKSEKGGNIDLLPNGNYLINMGSLNRCIEVTKDGK
                     VVWDVFEEGLAPDKVTWGPFRQYRSHCYSSLYPCYFTSNISGKISSTIPLVINNEGTE
                     SDSYIVECKQKDGTWMKVAGTQQV"
     gene            complement(1598..2617)
                     /locus_tag="VFU15_11010"
     CDS             complement(1598..2617)
                     /locus_tag="VFU15_11010"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEU4718357.1"
                     /translation="MRSIQFIGTQRSGSNLLRVMLHQHPLICAPHPPHFLHILLPLVS
                     MYGDVKDDANFYSMIDDACTLAETNPVSWNLVFDRDEVRSRVRVRTLADVARVLYEIK
                     AEAKGSGYWCCKSMANVHYIDKLGEAGIRPFFIYIFRDGRDVALSFRGTVIGEKHVYH
                     LAQQWKKEQELSLDICAAAGPGNSAIVRYEEFISGPEAALKNICARIDVAYDPRMLAF
                     NESDESKETAASGGMWSNVAKPVIRSNAGKFRSGLSAEDIRIFESVAGDMLGRLGYTP
                     EFPAEERRSFTENEMFFFTEENRRMKREAKQNASPSDIAHREKQETFIASLKEKYTNY
                     VNTLA"
     gene            complement(2664..3842)
                     /gene="chrA"
                     /locus_tag="VFU15_11015"
     CDS             complement(2664..3842)
                     /gene="chrA"
                     /locus_tag="VFU15_11015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HEU4718358.1"
                     /translation="MISLGQLFFVFLKIGATAFGGNVALVAAVRKELCEKKHYLKDAQ
                     VLDFMTLGNLMPGPLAVNVVAAIGYTLRGMLGAIVATIAVLIPSFFLVSFLADMYFRY
                     GSSPVVNEIFSGLLPGVCAIIAATAWSLAKKNISSVLQVGIVVLAGAAILFFHGFLTT
                     LVIIAVSGLLGLFLIRKKEGPEFMGPPKEKYSPWPLVAMTVLVLAGIILWLIHPEGEH
                     AQKTRLLGLSFGGMSVTLFGGGYVFIPAIEKVVVGMHHWVTSKEFADGIAMGQVTPGP
                     ISISAAFIGWRVAGFLGALVSTVAIFFPPAFLMVVAQQFVDRVKKHPVAEAVFSGVRP
                     AVIGMIVASVYVLSKSSPLDWQAFFIFAFVLTLSVWKNVDSALLILASGFFGWALHLF
                     "
     gene            4025..6454
                     /locus_tag="VFU15_11020"
     CDS             4025..6454
                     /locus_tag="VFU15_11020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019943851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEU4718359.1"
                     /translation="MWEKLSHFILRNRIAILVVMLCVTVFMGVVAYKRLELSYVYARV
                     LPVDDPKYIAYENFKQRYGEDGTVLVMGFQDSAMFRLDKFQGWYDLTESIRQIDGVKN
                     VMSITKIYNLVRNDSLEKFDTKPVVSRRPQTQEEVDSLKRQILRLPIYEGLIINRKDN
                     VTVMAVTFKEKDLNSSRRTHIVDLIREQSQQFAQKYHEELHYSGMPWIRAEYMRKIGG
                     ESAFFLVLSVLVTALILFFFFRYFNAVFFSVIIVLVGLTWSVGTIGLFGYKITILTGL
                     IPSLIVIIGIPNCIFLINKYQEELLKHGNKTKALARMVQKVGLSNFLANITTSIGFAV
                     FYFTNSSMLVEFGVVAAINVMTTYMIALVLTPVIFSFLPPPKPKQMKHLSGKNINKLL
                     DWINKLVHHHRPAIFITITILTIISALGFLRVKLNGYLVDDLPKNDPIYTDLRFFEHH
                     FKGVLPFEIAIEVKDSKDSLAPKNLFDHDGEVLYNIDRLQDSISSRPEFSRPVSIVEG
                     LKFSYQALKNGNPKAYRLPNNHLDLKELADYSGTVSNKQQMLDAFIDSTHKFTRISFQ
                     MADIGSDSSEKVFAAIKPVVDSLFPASQFKVDITGYSVMFLRSFHYMLHHLFVSLLIA
                     IVLILLLGMVLFRSVWIIVLSKLPCLIPLVMTAGIMGYLGIPFKSSTILIFSIAFGIA
                     SDGTIYILTEYRHQLRKLSASKASKAVSRAIYETGLSMVYTNIILFAGFLIFLLSSFG
                     GTVALGILLSITLMVSLLTNLIMLPSILLSLEKRTATRAFMKEPLIDIYDEEEDIDSE
                     KLQIKNHHSHE"
     gene            6544..>6647
                     /locus_tag="VFU15_11025"
     CDS             6544..>6647
                     /locus_tag="VFU15_11025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEU4718360.1"
                     /translation="MKGIILAGGSGTRLHPLTLAVSKQLMPVYDKPMI"
BASE COUNT         1534 a         1926 c         1699 g         1488 t
ORIGIN      
        1 gacctgctgc gtcccggcga ccttcatcca tgtcccgtct ttctgtttgc attccacgat
       61 gtagctgtcg ctttccgttc cttcgttgtt gatgacgagc gggatcgtgg aagaaatttt
      121 cccgctgatg ttcgatgtga aatagcaggg atacagcgaa gaataacaat gcgaacggta
      181 ctggcggaaa ggcccccacg ttactttgtc cggcgcaagt ccttcctcga acacatccca
      241 caccactttc ccgtctttcg tcacctcgat gcagcggttg agcgagccca tgttgatgag
      301 gtaatttcca ttgggaagca ggtcgatgtt cccgcctttt tcacttttgc cgtcgctgag
      361 cgaatcgaac ctgcacggga acgaccagac gatgcggcac gaatcgccgg gagatgccgg
      421 ctgtgaaaaa atgagcacgc gcgaagatat tcccggatcg gcgatggaat cattgtcgaa
      481 aacagcgatc gttccgtcgg gctggatgga agcatcgtgc tggtgccagt aaaacccgtt
      541 ggcgtatttc gcttcgccgg acggcatccg tttcccgtac gcctgcacgg cttttcctgt
      601 gcggtaatcc actttcacga tgcggctcat gtcacggaag cccgcgtata cgaaatgcat
      661 gctgtcgtcg acgctgaatg cgttcatgtg cgtggagaca tccggcttcc cgtcgggcgt
      721 tttgcgtgaa aagatatctg catcggtaaa ataattttcc gcttcccatt tccacaccat
      781 ttttcccgag cggtcgaatt cttccaccgt tccgaaaaca atacgcacgt acgtcgaatc
      841 accgggaact ttccgcatca ccgcgtgatt gcccatcacc atgaaatttc cattgggaag
      901 ccgttcgaac ccgtgatggt aatgctgcga cgtgtcgcgc gggttctgcg gcgctttcca
      961 catcgtgcgc ccgtcgacgg aaacctcgaa agcatttttt tccgttatga acgtaaacgt
     1021 cccggctttg gaaatgcgga gatcacgcac ttcatcgtca cgcacgaaat cgcctttccc
     1081 ggaagacggc aggaaccaca caggcttccc gtcccggccg gtgatgacct cggcataatc
     1141 gaaggaaacg agcccgccct gcgtttttgc ggaatcattc ttcacgacgc ggatgcggct
     1201 gccttccggc agcggaagta tgctgaagcc ataccaaggc gacgttgcga tctttttctt
     1261 cttcgcgccg gtcgaaacat aattccacgc gtattctttc ccgaaaccga aaccgctgat
     1321 catcgtcgca ttcgtgctgt ccgttacgct gacgaacggt acggtgtctt ttccattcac
     1381 cgaaaaaaca ttcaccacgt aatacactgc tttttccgta acgggatgct cgaacatgac
     1441 ctgcgtgtaa ttcagcttgc tgccgggaac gggaaggatg ggcgaggaag gaataaacgg
     1501 ctcgcggatc accactttgc tcagcgagaa cggtgtcacc tgggcgaacg cgtttagtgc
     1561 tacaaaaaag aaaagcaatg cggctgttcg cttcatttca ggcaagggtg ttgacgtagt
     1621 tcgtgtattt ttctttcagc gaggcaataa aggtctcctg cttttcccgg tgcgcgatat
     1681 cggacggcga cgcgttttgt ttcgcctctc ttttcatcct ccggttttct tccgtgaaaa
     1741 aaaacatctc gttttcggta aaggaccttc gctcttctgc gggaaattcc ggcgtgtagc
     1801 cgagacgccc gagcatgtcg cccgccacgg attcaaaaat gcggatatcc tcagcggaaa
     1861 gcccgctcct gaattttccc gcattgctgc ggatcaccgg tttggccacg ttgctccaca
     1921 tcccgcccga cgcagccgtc tctttcgact catccgattc gttgaatgcc agcatgcgcg
     1981 gatcgtacgc aacatcaatg cgcgcacaga tgtttttcaa tgccgcttcc gggccgctga
     2041 tgaattcctc gtaacgcact atcgctgaat ttccgggacc ggccgctgcg cagatgtcca
     2101 gcgaaagctc ctgctctttt ttccattgct gcgcaagatg gtaaacatgt ttttccccga
     2161 tcaccgttcc gcggaacgaa agcgccacgt cgcgcccgtc gcggaaaata taaatgaaga
     2221 acgggcgaat accggcctcc ccgagcttat cgatgtaatg cacgttcgcc atgcttttgc
     2281 agcaccagta accggaacct ttcgcctccg cctttatttc atacagcaca cgcgcaacat
     2341 ccgccagcgt gcgcacgcgc acgcgcgacc tgacttcgtc gcggtcgaac acgaggttcc
     2401 agctcaccgg gttggtttcc gccagcgtgc aggcatcatc gatcatgctg taaaagttag
     2461 catcgtcctt cacgtctccg tacatggaaa cgagcggcag caggatgtgc aggaagtgcg
     2521 gaggatgcgg tgcgcagata agcgggtgct ggtgcagcat tacacggaga agattcgaac
     2581 cggaacgctg cgtgcctatg aactggatgg atctcaaaat gaaattttta tttctgacgg
     2641 cgcagtgcgc aaagatacgc ttctcagaag agatgaagcg cccatccgaa aaatcccgaa
     2701 gcgaggatga gcagggcgga atcgacattt ttccagacgg aaagcgtaag cacgaacgcg
     2761 aaaatgaaga acgcctgcca gtcgagcggg gaagatttcg acagcacata caccgatgcc
     2821 acgatcatcc cgatcactgc gggacgcaca ccggaaaaga ccgcttcggc cacgggatgt
     2881 tttttcacgc ggtcgacaaa ctgctgcgca accaccatca ggaatgccgg cggaaaaaat
     2941 atcgccacag tggaaacaag cgcgcccagg aatcccgcca cgcgccagcc gatgaatgca
     3001 gcgctgatgg agatcggtcc gggcgtcacc tgccccatcg cgatcccgtc cgcaaattcc
     3061 ttcgatgtca cccagtggtg catgcccacc acgacttttt caatcgcggg aatgaaaacg
     3121 tagccgccgc cgaaaagcgt cacgctcatc ccgccgaagc tgagcccgag caggcgtgtt
     3181 ttctgggcat gttctccttc gggatgaata agccagagaa tgatgccggc cagcacgagc
     3241 acggtcatag cgacgagcgg ccagggtgaa tatttttctt tcggcggacc catgaattcc
     3301 gggccctctt ttttgcggat caggaaaagt ccgaggagac ccgaaacagc aataataacg
     3361 agcgtggtga ggaaaccgtg gaagaaaaga atggccgcac cggcgagaac gacaatgccc
     3421 acctgcagca cggaagaaat attttttttc gccagcgacc aggccgttgc cgcgatgatc
     3481 gcgcagacgc cgggaagaag gccggagaat atctcgttca ccaccggcga ggagccgtaa
     3541 cggaaataca tatccgcgag aaagctgaca aggaaaaaag aagggatcag cacggcgatc
     3601 gtcgcgacga tcgcacccag cattccgcgc aacgtatacc ctatggcggc cacgacattt
     3661 accgcaagcg gtccgggcat gaggttaccg agcgtcatga aatcgagcac ctgcgcatcc
     3721 ttcaggtaat gctttttttc gcagagctct tttcgcaccg ccgccaccag cgccacgttt
     3781 cccccgaagg cggttgcacc tattttcagg aagacaaaaa aaagctgccc gagagaaatc
     3841 attttgcaaa tatgtacata atgctccgcg ccttatgtaa cccgggtcat gtatttttaa
     3901 attaccgtgc cgggaacggg tttgcacgcg gttttaaaga aatccgctcc ggcaaatcat
     3961 tccgaaatat gtacttttac cctcgtctgt cattgacgga aaacgggacc tgtccccgaa
     4021 gcttatgtgg gaaaaacttt ctcatttcat tctgcggaac cgtatagcca ttctcgtggt
     4081 gatgctctgc gtcaccgtgt tcatgggggt cgtggcgtac aagaggctgg agctttcgta
     4141 tgtgtatgcg cgcgtcctcc cggtggacga tccgaaatac atcgcgtatg agaatttcaa
     4201 gcagcgttac ggggaagacg gaaccgtgct ggtcatgggc ttccaggatt cggcgatgtt
     4261 ccggctcgac aagttccagg gatggtacga cctgacggaa agcatacgcc agatcgacgg
     4321 cgtaaagaac gtgatgtcga tcacgaagat ctacaacctt gtccgcaacg acagcctgga
     4381 aaaattcgac acgaaacctg tcgtgagccg gcggccgcag acgcaggagg aagtggacag
     4441 cctgaagagg cagatcctgc gccttcccat ttacgaaggg cttatcatca acaggaagga
     4501 caacgtaacg gtgatggcgg ttacgttcaa ggaaaaagac ctgaattcga gcaggcgcac
     4561 gcacatcgtc gacctgatac gagagcagtc ccagcagttc gcgcagaaat accatgaaga
     4621 gcttcactat tcgggcatgc cctggattcg tgcggaatac atgcgcaaga tcggcggtga
     4681 atcggcgttc ttcctcgtgc tctccgtgct cgtcacggcg ctcatccttt tctttttctt
     4741 ccgttacttc aacgccgttt tcttttcagt gatcatcgtg ctcgtcggcc tcacgtggtc
     4801 ggtgggaacg atcggcctct tcggctacaa gatcacgatc ctcacgggac ttattccttc
     4861 gctcatcgtc atcatcggca taccgaactg cattttcctg atcaacaaat accaggaaga
     4921 gctgctgaag catggcaaca aaaccaaggc gctcgcgcgc atggtgcaga aggtcggcct
     4981 gtcgaatttc ctcgccaaca tcaccacttc gatcggcttc gccgtattct atttcacgaa
     5041 cagctccatg ctcgtcgaat tcggcgtggt ggcggccatc aacgtgatga caacgtacat
     5101 gatcgcgctg gtgctcacac cggtcatctt cagcttcctg ccgccgccga aaccgaaaca
     5161 gatgaagcac ctcagcggga agaacatcaa caagctgctc gactggatca acaagctggt
     5221 gcatcatcac cgccccgcga tcttcattac gatcaccatc ctcaccatca tcagcgcgct
     5281 cggtttcctg cgtgtgaagc tcaacggcta tctcgtcgac gatcttccga agaacgaccc
     5341 gatctacacc gacctgcgtt tcttcgagca tcatttcaaa ggcgttcttc ctttcgagat
     5401 cgcgatcgag gtgaaagact ccaaagattc gcttgcgccg aaaaatcttt tcgaccatga
     5461 cggggaggtg ctgtacaaca tcgaccgcct gcaggattcc atttcgtcgc ggcccgaatt
     5521 ttcacgtcct gtttccattg tcgagggact caaattttcc taccaggcgc tgaagaacgg
     5581 gaacccgaaa gcgtaccgcc ttccgaacaa ccacctcgac ctgaaagagc tggccgacta
     5641 cagcggaacc gtgagcaaca agcagcagat gctcgatgcg ttcatcgaca gcacgcataa
     5701 attcacgcgc atcagcttcc agatggcgga catcggctcg gacagctcgg aaaaagtttt
     5761 tgcggcgatc aaacctgtgg tcgattcgct cttcccggct tcccagttca aggtcgacat
     5821 caccggctat tccgtgatgt tcctgcgcag cttccattac atgctgcacc atcttttcgt
     5881 gagcctgctc atcgccattg tgctcatcct gctgctcggc atggtgctgt tccgttcggt
     5941 gtggatcatc gtgctttcga aacttccctg ccttatcccg ctcgtgatga cggccggcat
     6001 catgggctat ctcggcattc cgttcaagtc gtccaccatt ctcatttttt ccatcgcctt
     6061 cggcatcgca tccgacggaa cgatctacat cctcaccgaa taccgtcacc agctgcgcaa
     6121 gctttccgca tcaaaagcct cgaaagcggt ttcacgcgcg atctacgaaa cgggactgag
     6181 catggtgtac acgaacatca tcctcttcgc cggcttcctc attttcctgc tgtcgagctt
     6241 cggcggaacg gtggcgctgg gcatcctgct ttcgataacg ctcatggttt ccctgctgac
     6301 gaacctcatc atgcttccga gcattcttct ttcgctcgaa aaacgtacgg ccacgcgcgc
     6361 tttcatgaaa gagccgctga tcgatattta cgacgaagag gaagacattg actcggaaaa
     6421 gctgcagatc aaaaatcatc actcccatga atagctattc ccggctcacg gttttcagcc
     6481 tggcctgaac gccgcgttcc agaacccgcg ccgggaaacg caaaaccgaa aacagcaccc
     6541 tccatgaaag gaatcatcct cgctggcgga tccggcacac gacttcaccc gctgacgctc
     6601 gccgtcagca agcagctgat gcccgtctac gacaagccga tgatcta
//
LOCUS       DASTSF010000387         4116 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1001862,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000387 DASTSF010000000
VERSION     DASTSF010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4116
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1001862"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1926)
                     /locus_tag="VFU15_11030"
     CDS             complement(<1..1926)
                     /locus_tag="VFU15_11030"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU4718361.1"
                     /translation="MKKIFFCLLLLFPLCIFSQNSIRGKITDEKTGEALVGATVFIPD
                     LHTGASTDTSGRFIIHNVPGGSFLAEIHMTGYTAKAIVLSSNDSANYIVITLSQKPTE
                     MRAVVITSPSGSTDRQTNPVPTVIAGRDHLLAGSSTNLVAGISNIPGISQVSTGSAVS
                     KPVIRGLGYNRVVVLRDGIRQEGQQWGDEHGIEIDEFEVDRIEIIKGPGSIMYGSDAM
                     AGVINFMTPKPVDDGKWSGEFMTNYQTNANLAGLSFSEAGNFHGVSWMTRYSQKKAGN
                     FSTPVDGHVLNSGFDEYDASGFLGLNRKWGYSRISFSTFNQRIGMPEGDRDSLGIFTK
                     EIRVDDSTTTLVSVPESARKGFDNSILTPFQRINHNRVVWDNAFYFAHSKLTLTGGWQ
                     QNRRREFGDVFSPDEAGLHFLLTTANLDVKYFFPAVRQWSITSGASGQFQQSSNLGTE
                     FLIPAYSQYDAGAFVFASRHFGKLFFSGGARADMRLLNASALYLDANGNAVDATNSVM
                     TKFDAFSKNFYAFSGSLGLSYHISGKSVLRVNLSKGFRAPNLAELGANGVHEGTFRYE
                     KGNHALNPENSYQLDAGFSCDADHVSFELDGFYNRITQYIFLQKVNSVSGGDSIIDPA
                     DPVPLFTFVQGNAALYGG"
     gene            2169..2489
                     /locus_tag="VFU15_11035"
     CDS             2169..2489
                     /locus_tag="VFU15_11035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718362.1"
                     /translation="MRSPLRQLVSLFLLAVFLFALEGKVMHDFAHRDDFHCWANGEKH
                     FHGEMHSCAICDFIFAPADGISSADVCSKNNLYFKITSASPLWDCYSLPVTSLFLLRA
                     PPVS"
     gene            2595..>4116
                     /locus_tag="VFU15_11040"
     CDS             2595..>4116
                     /locus_tag="VFU15_11040"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU4718363.1"
                     /translation="MKKQVDYFLLFILLVQCLSGFDARAQQNCDAVFSGKIMCSLHHE
                     PVGFAVVYLSDINRGATASDSGYFRIEGICPGDHHVRISFVGFITIDTSLYIPQQKQV
                     VFDMLPSGDLKVFDVRDHKIKNQDVVSLQRNDLAGKDIEQTRGLTLAESLKSITGMNS
                     IQTGPSISKPMIHGMYGNRVLIMNNGIRMEGQNWGADHAPEIDPFVAAKLSVIKGAAS
                     IRYGPDAIAGVVLVEPKAMPAARCLRGDINLVGASNGRSGATSGMMEGAFGKKFTGLS
                     WRVQGTMRQAGSFSTPHYYLTNTALKENDYSGSLVYSHANFGAKVFYSSFNSTIGIYS
                     GSHVGNLSDLLFLFNSPQPVVPSVFSYDIKRGYQVVHHNTIKAEGFIDLKKAGKLTYT
                     FARQENKRSEFSEDVSYNQSIVDQNIPDSYFQLITHTSDLIWEHKPVHHITGSIGLSY
                     ITQGNVYKGLDFRALLPNYRNYGGGAFALEKWNSEKLTLEAGIRFDYLWMRTYTEDF"
BASE COUNT         1006 a         1006 c         1073 g         1031 t
ORIGIN      
        1 gcctccgtaa agcgccgcat ttccctgcac gaatgtgaac agcggaacag ggtccgcagg
       61 atcgatgatg gaatcgccgc cggaaacgga attcactttc tgaagaaaaa tgtactgcgt
      121 gatgcggttg taaaacccgt ccagctcgaa ggaaacatga tccgcgtcgc aggaaaagcc
      181 ggcgtccagc tggtagctgt tctccgggtt cagcgcgtgg ttcccttttt cataacggaa
      241 tgtcccttcg tgtacgccgt tcgcaccgag ctcggcaaga ttcggcgcac ggaacccttt
      301 cgaaagatta acgcgcagca ccgatttacc ggaaatgtga tacgataaac ccaggctgcc
      361 ggagaaggca tagaaattct tcgagaatgc atcgaacttc gtcatcacgg aattcgttgc
      421 gtcaacggca ttcccgttcg cgtcgaggta aagtgccgag gcgttcagca ggcgcatatc
      481 tgcgcgcgca cctccgctga aaaataattt tccgaaatgc cggctggcga aaacgaaagc
      541 gcctgcgtcg tattgcgaat acgcgggaat aagaaattcc gttccgaggt tgctgctctg
      601 ctggaattgc ccgctcgcgc cggacgtaat gctccattgc ctgacggccg ggaaaaaata
      661 tttcacgtcg aggttggccg tagtcagcag gaagtgaagc ccggcttcat caggagaaaa
      721 tacatcgccg aattcacgcc tgcggttctg ctgccatccg cctgtcagtg tcagtttcga
      781 atgcgcaaaa taaaacgcgt tgtcccacac cacgcggttg tgattgatgc gttgaaatgg
      841 tgtgaggatg ctgttgtcaa atcctttccg cgcactttcc ggaacggaaa ctaaagtcgt
      901 ggtggaatcg tccacgcgta tttcctttgt gaaaatcccc agcgaatcgc ggtcgccttc
      961 cggcatgccg atccgctggt tgaacgtgga aaagctgata cgtgaataac cccatttccg
     1021 gttgagcccg aggaaaccgc tggcgtcgta ttcgtcgaaa ccggaattca gcacatgccc
     1081 gtcgaccggc gtggaaaaat tccccgcttt cttctggctg tatctcgtca tccagctcac
     1141 accgtgaaaa ttcccggctt cggaaaaaga aagcccggca aggttcgcat tcgtctggta
     1201 attcgtcatg aattcacccg accattttcc gtcgtccacc ggtttcggtg tcatgaaatt
     1261 gatgacgccc gccatcgcat ccgaaccgta catgatgctg cccggacctt tgatgatctc
     1321 gatgcggtcg acttcgaatt cgtcaatttc aattccgtgc tcgtcgcccc attgctgtcc
     1381 ttcctgccgg atgccgtcgc gcagaacaac aacgcggttg tagccgagac cgcggatcac
     1441 cggtttggaa accgcgcttc ccgtgctcac ctggctgatg ccgggaatgt tcgagattcc
     1501 ggcgacaaga ttcgtggatg aaccggcgag aagatggtcg cgtccggcga tcaccgtggg
     1561 aacggggttt gtctgccggt cggtggaacc ggatgggctc gtgatcacca cggcgcgcat
     1621 ttccgtcggt ttctgcgaaa gtgtgatcac gatgtaattg gcggaatcat tcgaggaaag
     1681 cacgatggct ttcgcggtgt aaccggtcat gtggatctcg gccaggaaac ttcctccggg
     1741 aacattatga ataatgaagc gcccgctcgt gtcggtgctg gcaccggtat gcagatcggg
     1801 aataaaaacg gtggcgccaa cgagcgcttc accggttttt tcatcggtga tctttcccct
     1861 tatgctgttc tgtgagaaaa tgcataacgg gaataacagg agaagacaga aaaatatttt
     1921 tttcatggaa acatcattga acagacccgg caggcttccc ggagagataa taaaagggcg
     1981 cagagaatta cgcagcagga agttttcggc acgaagttat ccaataatca aaaaggttta
     2041 acgcgatttt ttaaagtaaa cgtcatagga gaaagtttct gctccctgcc gggcgccatt
     2101 ggtcacggtt taagtccgtt taaagcgata cgggaacggg tgcaggataa ttcgtaactt
     2161 tacatgaaat gagatcgcct ctgcgacaat tggtttcttt attccttctg gctgtttttt
     2221 tatttgcgct ggaggggaaa gtgatgcatg atttcgccca tagggatgat ttccattgct
     2281 gggcgaatgg agaaaagcat ttccacggtg aaatgcattc ctgtgccatc tgcgatttta
     2341 tttttgctcc ggcagacgga atttcttcgg ccgatgtttg ttctaaaaac aacctgtact
     2401 tcaaaataac ttccgcaagt cctttgtggg attgttattc tctgccagtc acctctttat
     2461 ttctgctgag ggctccccct gtttcctgat ctcgcatatt caggttctct ttaatttttc
     2521 tgtatggtca tcgtccgcac acgatcgtgt tcgttcggtg ttcggggtgc aagtactcca
     2581 aattactttt tgcgatgaaa aagcaggtgg attatttcct tttgttcata cttctggttc
     2641 agtgcctttc cggtttcgat gccagggcgc agcaaaactg cgatgccgtc ttctccggaa
     2701 agatcatgtg ttcattgcac catgaaccgg tagggttcgc cgttgtttat ttaagcgaca
     2761 taaacagggg cgctacagcc agcgattcag gctatttcag gattgaaggt atctgcccgg
     2821 gcgaccatca cgttcgtatt tctttcgtcg ggtttatcac gatagatact tcattatata
     2881 tcccgcagca gaagcaggtt gttttcgata tgcttccttc cggtgacctg aaggttttcg
     2941 atgtcaggga ccataagatt aaaaaccagg acgtcgtcag ccttcagcgg aacgatctgg
     3001 ccgggaaaga tatcgaacag acaagaggat tgacactcgc tgaatcgctg aaatcaataa
     3061 cagggatgaa cagcattcaa acgggtccgt caatttccaa gccgatgatt catgggatgt
     3121 acggcaaccg cgtactgatc atgaacaatg gaatccgtat ggaaggccag aactggggag
     3181 cggatcatgc cccggaaatc gatccgtttg ttgccgcgaa gctgagcgtc atcaaaggtg
     3241 cggccagcat acgctatggt ccggatgcaa tcgcgggagt cgttcttgtc gaaccgaaag
     3301 ccatgccggc agcaaggtgc ctgcgcggag atattaatct tgtcggggcc agcaacggcc
     3361 gctcaggagc cacgtccgga atgatggaag gagcgttcgg aaagaaattt acgggattga
     3421 gctggcgcgt tcagggaacg atgcggcagg cgggaagttt cagtacgcct cattattatc
     3481 ttacgaatac cgcgctaaaa gaaaacgatt attcaggatc gctcgtttat tcgcacgcaa
     3541 atttcggagc aaaggttttt tacagcagct tcaattcaac tatcggtatc tattcaggtt
     3601 ctcatgtggg aaatctcagt gatctgcttt tcctgttcaa cagtccgcag ccggtagtgc
     3661 cgtctgtatt ctcttacgat atcaaacggg gataccaggt cgttcaccac aatacgatca
     3721 aagccgaagg ttttattgat ttaaaaaagg cgggaaagct gacctacaca ttcgcccggc
     3781 aggaaaacaa acgttcggag ttcagcgaag atgtttccta taaccagtcg atagtcgatc
     3841 agaatattcc cgattcgtat ttccaactca tcacccatac gtccgatctc atctgggagc
     3901 ataagccggt tcatcacatt acaggaagca tagggttgag ttatatcacc caggggaacg
     3961 tttataaagg attggatttt cgtgcattgc ttccgaatta caggaattac ggaggcggag
     4021 cgttcgcact cgaaaaatgg aacagcgaaa aactcaccct cgaagccgga atacggttcg
     4081 attacctgtg gatgagaaca tatacggaag atttca
//
LOCUS       DASTSF010000388         8376 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1458003,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000388 DASTSF010000000
VERSION     DASTSF010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 8376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8376
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1458003"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            417..2195
                     /locus_tag="VFU15_11045"
     CDS             417..2195
                     /locus_tag="VFU15_11045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007139628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEU4718364.1"
                     /translation="MSRFLRVPERLIELLHDKLDKKQFLILSSILVGLTAGLSAVALK
                     VCVHFLHGWITGYARQGHNIYYAAAPLIGIFLSVLFVRIHLKGKLGRGNANILYSIAK
                     KSSNLPRDQMYSHLVTSALTVGLGGSAGLESPIVTTGSAIGSNFGKTYKVTYKDRTLL
                     LASGAAAGIAAAFNSPVAGVLFALEVLLVDVTISAFIPLMIAAAAGALCSRIILNEKI
                     LFVFNLRQPFDYHNVPWYMILGLLCGVGAVMYSRIFPRIEGLFKKKRENPFPNAIIGG
                     LILSVLILFLPSLFGEGYDSIKYLADRRTSLLTADSILGTAIHSPIWILVFITVVMLF
                     KVVATAVTIGSGGNGGNFAPSLFVGGYLGFIFSYGITLTGITTLPVANFTLVAMAGML
                     TGIFHAPLTGIFLIAEITGGYELMIPLMIVSALSYSLVRYFEPFSMDTKKLAKKGHIF
                     THDKDRNILSTLKVRKIIETDFQPVQPDMNLGQLVDIVAKSHRNIFPVVTKYGELEGI
                     IMLDSIREIMFQREKYDAVTVRQLMRPPAAIVEPDENMVRVMKKFDETGAWNLPVVEN
                     NVYIGFVSKSRIFTNYRSRLITASGD"
     gene            complement(2198..3064)
                     /locus_tag="VFU15_11050"
     CDS             complement(2198..3064)
                     /locus_tag="VFU15_11050"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HEU4718365.1"
                     /translation="MSSSPAPVLLFNGEQKSSSTPLFTSHNRAFRYGDGVFESMRVAG
                     GKLAFSARHLKRLMAGVNLLQLRLPDNFVSKSLDDWCMELCRLNELTGGARARLSVFR
                     NDGGYYNPLTNEASWLLELWPYETEAYSLNDKGLAIELYQDIRKPVNKLSGIKSSNSE
                     LYVLAALYAKSMNVDDSVLINQSGNVIEATGSNLFAVKNGVLYTSPVSEGCVAGVMRS
                     VIMDIAQENRIAVYEVPLPLSVLLNSDEIFLCNAVRGIQWVGTYRAKRYSNAMAKKLM
                     ELINKKAMPGTV"
     gene            complement(3089..3739)
                     /locus_tag="VFU15_11055"
     CDS             complement(3089..3739)
                     /locus_tag="VFU15_11055"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEU4718366.1"
                     /translation="MMQPLLTYALIAGTCLISLMNMNNREFKMRFMFSAYAIDKNREW
                     WRFFTGGFLHADFGHLLFNMFSFYFFGTLVEQTFKMEFGMGIGTLMYLALYVTSLPAS
                     NIYTYFRQRDNPNYFALGASGAVSAVIYASILVYPLQHIFIYFISVPAWIFGIVYLGI
                     SWVMARNKSDNIGHDAHFFGAVYGFLFPAIFNPSLLSRFVEQIEMFFRFGGNFFGH"
     gene            complement(3747..4292)
                     /locus_tag="VFU15_11060"
     CDS             complement(3747..4292)
                     /locus_tag="VFU15_11060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017186353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1572 family protein"
                     /protein_id="HEU4718367.1"
                     /translation="MVSSGAEKQFLENALFEFSRMKKSGDAVLSRLNEKEIDFSFSAN
                     DNSVRVIVQHMAGNLLSRWTDFLTSDGEKSWRNRDAEFEAQALAKAELVEKWEEGWKT
                     LFSALENLTEKDVLREVTIRNEKLTVMQAIFRQISHYAYHTGQLVLIAKHIRGEEWQS
                     LTIPKGKSAEAAGKGTYLSKP"
     gene            complement(4286..4702)
                     /locus_tag="VFU15_11065"
     CDS             complement(4286..4702)
                     /locus_tag="VFU15_11065"
                     /inference="COORDINATES: protein motif:HMM:NF044571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718368.1"
                     /translation="MKKIFSIALLMLIAVTGFSQELRNSSGSYWGQVESDGTVRNSSG
                     SYRGKFESGGDIRNASGSYVGHIDSDGTIRNASGSYMGKVESDGTVRSDSGSYLGKIE
                     DDGDVRNDSGSYVGSAKGVDKAQAACLFFFFNMETW"
     gene            complement(4748..7093)
                     /locus_tag="VFU15_11070"
     CDS             complement(4748..7093)
                     /locus_tag="VFU15_11070"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU4718369.1"
                     /translation="MSCAKKSGSSHAAVSWSSGVAGLLRDTAHVGPVYDSLRRDVLSA
                     KNDTLAIRALYQLAEHSSADRLLRFASEAGLQSERIGYEAGRINAVFFRGVYHYKKSA
                     YDSALACYDSALVMAQRAHRLDIQAASLARKGEVYYMEDEDAKAYDCLDQGLIIAQLS
                     GDHHTEAFCLGHIGEVFRMQDKNEQALEYYGKAMKIATARNDREVTSYCLGSMGDVFR
                     SQGNYSKSLDCYQQSLAIARQLGAKYDVAYALSSIGDVNRLLQNFREAIDYYLQALDS
                     FRELNDNTNTGYCLSSIGDVYRMENDFPNALKYSREAEQYMLKTGDKLEIANCLSSIG
                     AVFSMHNDYDSALKYLQMSIDTAKISNDKSTMAYATRNIADIYFRMGKQPDALRLGEQ
                     GMQFAQETRKAMEIKGAAELLFRIYEKNGDYKKALDMHKLFVQLKDSINSNDQVKKFA
                     AVKYKAKEEEMKAGEAKKDAEYQAERIKRQEALNRQKTIAYAVSGGLLLVVVLAIVIL
                     RSLRQSRRDKAAIEQQKHLIEEKQKEILDSITYAKRLQEAILPPGKMVKDLFPESFVL
                     YLPKDIVAGDFYFLETSGNSIILAAADCTGHGVPGAMVSVVCSNALTRAVREFGIASP
                     EKILDKVRELVIETFEKSEDEVKDGMDISLVSVDLQQKELKWSGAHNPLWYLRDGAMH
                     EIKANKQPVGRQENSSPFTAHTIDIRKGDIFYLFTDGFADQFGGPKGKKFRYRQMEEL
                     LISVSDKKMEEQRQLLENAFREWKGGLEQVDDVCIIGVKFT"
     gene            7242..>8376
                     /gene="mnmE"
                     /locus_tag="VFU15_11075"
     CDS             7242..>8376
                     /gene="mnmE"
                     /locus_tag="VFU15_11075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011585413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEU4718370.1"
                     /translation="MNTNREDTIAALATAHGTAAIAVLRLSGPKAISITEELFHDKNG
                     KKKNLRDKASHTVHFGLIMEGEIVLDEVLVTLFRGPHSFTGEDVVEISCHGSVYIQQQ
                     LLNLLIRHGARLAQPGEYTLRAFLNRRFDLSQAEAVADLIASQSAASHQLAMSQMRGG
                     FSDKIRVLRENLVNFASLIELELDFSEEDVEFANRDDLKKLLFTIQNLIQRLVDSFEL
                     GNVIKHGIPVAIVGKPNAGKSTLLNTLLDEDRAIVSEIPGTTRDTIEDEIVIDGVLFR
                     FIDTAGLRDTTDVIESIGVSRSVEKMKASSIVIYLFDVREMTSGELKLALEELKKHIH
                     GAQLVIVGNKIDKKNIAEIQQEFAGFDVLFISAKEKLFIDDLER"
BASE COUNT         1836 a         2378 c         2162 g         2000 t
ORIGIN      
        1 ttcttgacct cggaaggcac cgactggaga atactttcat ttttaagatt gaagcgggaa
       61 gcgattgaag ccatgcccaa aaataaaaca tagcgaagcg accaaattga ctttagtcaa
      121 taataatttt tatgcctgta agtaaaattg cacccgaata gtttggttaa cctgacagcg
      181 cccccctcga aatcaagttt ttccggaggg cgcttttttg ccgggaagag ataagcgggg
      241 attgaatcat tcgcatcaac cggtactcct tttttcgcat cagccaggtg ctgctgatgc
      301 ttttcgaagt gtaagtcggg gattttttcc agttttcccc ggcttattac ttcacctccc
      361 gatccccttt ccctgcccgt gtcaatgtca atgcgtttac ttgctatttt tgcggcgtgt
      421 ccaggttttt aagagttccc gaacgtttga tcgagctgct gcacgataag ctggacaaaa
      481 aacagtttct tattctttcc agcatactcg tcgggctgac agccggcctt tccgccgtgg
      541 cgctgaaagt ctgcgtgcat ttcctgcacg gctggatcac cggctatgcg aggcagggcc
      601 acaatattta ttacgccgca gcgccgctga tcggcatctt tctctccgtc ctgttcgtgc
      661 gcattcacct caaagggaag ctgggccgcg ggaacgccaa catcctttat tcgatcgcga
      721 aaaaatcgag caacctgccg cgcgaccaga tgtattcgca tctcgtgacg agcgcgctca
      781 ccgttggcct gggcggatcc gccggtctcg agtcgccgat cgtgacaacc ggttcggcga
      841 tcggttccaa tttcgggaag acctataagg tgacgtacaa agaccgcacg ctgctcctgg
      901 ccagcggtgc ggcagccggg atcgcggcgg catttaattc tcccgtcgcc ggcgtgctgt
      961 tcgcgcttga agtgctgctg gtcgacgtca ccatttccgc tttcattccg ctcatgatcg
     1021 ccgcggcggc cggcgcgctc tgctcgcgca tcatcctcaa cgaaaaaata cttttcgtgt
     1081 tcaacctccg gcagccgttc gattaccaca acgttccgtg gtacatgatc ctcggcctgc
     1141 tctgcggtgt gggcgccgtg atgtactcgc gcatttttcc acgcatcgaa ggattgttca
     1201 aaaagaaacg cgagaaccct ttccccaatg cgatcatcgg cggactcatc ctttcggtgc
     1261 tgatcctttt tcttccttcg cttttcggtg aaggctacga ttccatcaaa tacctcgccg
     1321 accggagaac gtcgctgctg acggcagatt ccattctcgg cacggcgata cattctccca
     1381 tctggatact tgttttcatt acggtggtga tgctgttcaa ggtcgtcgca accgcggtga
     1441 cgatcggcag cggcgggaac gggggcaact tcgctccttc tcttttcgtg ggtggttacc
     1501 tcggttttat tttttcatac gggatcacgc tgacgggcat cacgacattg ccggtggcga
     1561 attttacact cgtggccatg gccggcatgc tcacggggat ctttcacgcg ccgctgacgg
     1621 gaatatttct catcgccgag atcaccggcg gatacgagct gatgatcccg ctgatgatcg
     1681 tctccgcgct gagctattcg ctcgtacgtt atttcgagcc cttctcgatg gacacgaaaa
     1741 agctggcgaa gaagggacat attttcacgc acgacaaaga ccggaacatc ctcagcacgc
     1801 tgaaagtgcg caagatcata gaaacggatt tccagcccgt gcagcccgac atgaacctcg
     1861 ggcagctcgt cgacatcgtg gcgaagtcgc accgcaatat ttttccggtc gtcacgaaat
     1921 acggcgagct ggaaggcatc atcatgctcg attccatccg cgagatcatg ttccagaggg
     1981 aaaagtacga cgccgtcacc gttcgccagc tgatgcgtcc ccccgcggcc atcgtggagc
     2041 ccgatgagaa catggtgcgc gttatgaaaa agttcgatga aactggcgcg tggaacctgc
     2101 cggtggtgga gaataacgtt tacatcggct ttgtttcgaa atcgaggata ttcacgaatt
     2161 accgctcgcg gctgatcacg gcttcgggag attgagccta taccgttccg ggcatcgctt
     2221 ttttgttgat cagctccatc agcttcttcg ccatcgcatt ggaatatctt tttgcgcggt
     2281 atgttcccac ccattggatt ccgcggacgg cgttgcagag aaatatttcg tccgaattca
     2341 gcagcacgct cagcgggagc ggaacttcat acaccgcaat acggttttcc tgcgcgatat
     2401 ccatgatcac gctgcgcatg acaccggcca cacagccttc cgaaacgggg ctcgtgtaca
     2461 gcacgccgtt cttcacggca aaaaggttcg agccggtcgc ttcaatgaca tttcccgact
     2521 ggttaatgag cacgctgtcg tcgacgttca tcgatttcgc gtacaacgca gcgagcacgt
     2581 ataattccga attggagctt ttgatgccgg agagcttgtt caccggcttg cggatgtcct
     2641 ggtacagctc gatggcgagt cctttatcgt tcagcgaata ggcttccgtt tcatacggcc
     2701 acagctcgag cagccaggac gcttcattgg tgagcgggtt gtaatagccg ccgtcgttgc
     2761 ggaacacgga aagacgcgca cgcgcaccgc ccgtgagttc gttcagcctg cacaattcca
     2821 tgcaccagtc gtcgagcgat tttgagacga aattatccgg cagccgcagc tggaggagat
     2881 tcactcccgc catcaggcgt ttcaggtgac gcgcagagaa agccagcttc ccgcctgcca
     2941 cgcgcatgct ctcgaacaca ccgtcaccat aacggaacgc gcggttatgc gacgtgaaca
     3001 aaggcgtgga agaacttttc tgctctccgt tgaaaagaag taccggcgcg ggagatgaag
     3061 acatattttt aaagataatg attcaggttc agtgtccaaa gaaatttcct ccgaaacgga
     3121 agaacatttc gatctgttca acgaacctgc tgagcagcga cggattgaag atggcgggaa
     3181 aaagaaagcc gtacaccgct ccgaagaaat gcgcatcgtg gccgatgtta tcggatttgt
     3241 tgcgcgccat cacccatgaa atgccgagat aaacgatccc gaatatccac gcgggcacgg
     3301 aaatgaagta aataaaaata tgctgcagcg ggtacaccag gatcgatgcg tagataacgg
     3361 cagaaaccgc gccggaagcg ccgagcgcaa aataattagg gttgtcgcgc tggcggaaat
     3421 aagtatagat attggaagcc ggcagcgaag tcacgtacag cgcaaggtac atcagcgtgc
     3481 cgatccccat tccgaattcc attttgaatg tctgctccac gagcgttccg aaaaaataaa
     3541 acgagaacat gttgaagagc aggtgcccga aatcggcgtg caggaaaccg cccgtgaaaa
     3601 agcgccacca ttcgcggttc ttgtcaatgg catacgcgct gaacatgaag cgcatcttga
     3661 actcgcggtt gttcatgttc ataagcgaga tcaggcacgt accggcgatc agcgcatagg
     3721 tcagcagagg ctgcatcata aatcgttcag ggctttgaaa gataggttcc tttcccggcg
     3781 gcttcagcag actttccttt cgggatggta agcgactgcc attcctcccc gcggatgtgt
     3841 ttcgcgatca gcacgagctg cccggtgtga taagcgtaat gcgagatctg ccggaagatc
     3901 gcctgcatca cggtcagctt ttcattgcgg attgtgactt cgcgcagcac gtctttttcc
     3961 gtgagatttt ccaacgcgct gaaaagcgtt ttccatcctt cctcccattt ttcaaccagc
     4021 tccgcttttg ccagcgcctg cgcttcgaat tccgcgtcgc ggttacgcca gcttttttcg
     4081 ccgtcggagg tgagaaaatc ggtccagcgc gacaacaaat tccccgccat gtgctggacg
     4141 atcaccctca cgctgttatc atttgcagaa aaagaaaaat caatttcttt ttcattcagt
     4201 ctcgacaaaa cggcatcgcc ggattttttc atccgtgaaa attcaaacaa cgcgttttcg
     4261 agaaattgtt tttccgctcc gctgctcacc acgtttccat attaaaaaag aaaaacaggc
     4321 aggccgcctg cgctttgtcc actccttttg cggaaccgac atagctgccg gaatcgttgc
     4381 ggacgtcgcc atcgtcttcg atcttcccga ggtagctgcc ggaatcgctg cgcacggtgc
     4441 cgtcgctttc cacttttccc atgtagcttc ctgacgcatt gcggatcgtc ccgtcgctgt
     4501 cgatgtggcc gacataagac ccggatgcgt tgcgtatgtc accgccggat tcgaattttc
     4561 cgcggtagct tccgctgctg ttgcgcacgg tgccgtcgct ttccacctgg ccccagtagg
     4621 agccggaact gttcctcagc tcctgtgaaa agccggttac ggcgatcagc atgagcaatg
     4681 caatggagaa gatctttttc atgtttttcg gtttgagaag taaagttaag cgaaagaccc
     4741 cagattatca ggtgaatttc acgccgatga tgcagacatc atcgacctgt tcgagacctc
     4801 ccttccattc ccggaaagcg ttttcgagga gctgtcgctg ctcttccatc tttttatccg
     4861 aaacagagat cagcagctct tccatttgcc ggtaacggaa tttcttccct ttgggtccgc
     4921 cgaactggtc cgcgaagcca tccgtgaaaa ggtaaaagat atctcctttc cggatatcga
     4981 tcgtatgcgc agtgaaagga gaagaatttt cctgtctccc tacaggctgc ttgttcgctt
     5041 ttatttcatg catcgcgcca tcgcgcaggt accagagcgg gttgtgcgcg cccgaccatt
     5101 tcagctcttt ttgctgcagg tcgacagaga caagggaaat gtccatgccg tctttcactt
     5161 cgtcctcgct tttctcgaac gtttcaatga ccagctccct gaccttatca aggatctttt
     5221 ccgggctggc aataccgaat tcccgcacgg cccttgtcag cgcgttgctg cacaccacgc
     5281 tcaccatcgc tcccggaacg ccgtgtcccg tgcagtcggc cgcggcaaga ataatgctgt
     5341 tgccggatgt ttcgagaaaa taaaaatcgc cggcgacgat atccttcgga aggtaaagga
     5401 caaagctttc gggaaaaagg tccttcacca ttttacccgg aggcaggata gcttcctgca
     5461 ggcgtttggc gtacgtgatg gaatcgagga tctctttctg cttttcctcg atgaggtgtt
     5521 tctgctgttc gatcgcggct ttgtctctcc tgctctgccg caggctccgc aaaataacga
     5581 tcgcaagtac gacaacaagc agcagtccgc cgcttacggc ataggcgatc gtcttctgcc
     5641 ggttgagcgc ttcctggcgc tttatccttt cggcctggta ttcggcatcc ttcttcgctt
     5701 cgccggcttt catttcttct tctttggcct tgtacttcac cgcagcgaat tttttcacct
     5761 ggtcgttgct gttgatgctg tccttcaact gcacgaacag cttgtgcatg tcgagcgcct
     5821 ttttgtaatc gccgttcttt tcataaatgc ggaacagcag ctcggccgcg ccttttattt
     5881 ccatggcttt ccgcgtttcc tgcgcgaatt gcatgccctg ttctccgaga cggagcgcat
     5941 ccggctgctt tcccatccgg aaataaatat ccgcgatgtt ccgcgtcgcg taggccatcg
     6001 tgcttttgtc gttgcttatt ttcgccgtat cgatcgacat ctgcaggtac ttcagcgcgg
     6061 aatcgtaatc gttgtgcatg gaaaaaacgg cgccgatcga gctcaggcaa ttcgcgatct
     6121 cgagcttgtc gcctgtcttc agcatgtact gttcggcttc ccgtgaatac ttcagcgcat
     6181 tggggaaatc attctccatc cggtacacat cgcctatgct cgacaggcag taaccggtat
     6241 tcgtgttgtc gttcagctcg cgaaaactgt ccaacgcctg caggtagtaa tcgatcgctt
     6301 ccctgaaatt ctgcagcagc cggttcacgt cgccgatgct gctgagcgcg tacgcgacgt
     6361 cgtatttcgc accgagctga cgggcaatgg caagcgactg ctggtaacag tccagcgatt
     6421 tcgaataatt tccctgcgag cggaaaacat cgcccatcga gccgagacaa tagctggtga
     6481 cctcccggtc gttccgggcg gtggcgatct tcatggcttt cccgtagtat tccagcgcct
     6541 gttcgttctt gtcctgcata cggaacactt ccccgatgtg acccaggcaa aacgcttccg
     6601 tgtgatgatc gccggatagc tgcgcgatga tcagaccctg gtccaggcaa tcatacgctt
     6661 tcgcgtcctc atcttccatg taatacactt ctcccttgcg cgccagcgaa gccgcctgta
     6721 tgtcgagacg gtgtgcgcgc tgcgccatta cgagcgcgga atcgtaacag gccagcgcgg
     6781 agtcataagc tgattttttg taatggtata cgccgcgaaa gaaaacggcg ttgatccggc
     6841 cggcttcgta acctatgcgt tcgctctgca gccctgcttc cgaagcaaac cggagcagcc
     6901 ggtcggcgga agaatgttcc gccagctggt aaagcgcgcg gatcgccagc gtatcgttct
     6961 tcgccgacaa aacatcccgg cgcaatgaat catagacagg accgacatgc gccgtatccc
     7021 gcagaagacc cgcgacaccg ctgctccagc tgacagcggc gtgcgatgac ccgctttttt
     7081 ttgcacacga caaaaaaaac aggcaacaca gcagaacgca gaagaccggc cgcatggcgc
     7141 caaaggtagt cagaacgcgg tacggcataa aaatcccgcg gttgaaccgg ttcagctctc
     7201 ccgctgcgga aaacctcttt ttcctacctt agttcgcacc tatgaatacc aaccgcgagg
     7261 acaccatcgc agccctggcg accgcacacg gcactgccgc cattgcggtc ctccgtcttt
     7321 ccggcccaaa agcgatctcc attaccgaag aacttttcca cgacaagaac gggaagaaaa
     7381 aaaatcttcg tgacaaagca tcgcacaccg ttcatttcgg actgatcatg gaaggagaaa
     7441 tcgtgctgga tgaagtgctc gtcactcttt tcaggggccc gcattcgttc accggcgagg
     7501 acgtggttga aatttcctgc cacggatcgg tttacatcca gcagcagctg ctcaaccttc
     7561 tcatccgtca cggtgcgcgc ctggcgcagc ccggagaata tacgctgcgc gctttcctca
     7621 accgcaggtt cgacctgtcg caggcggaag ccgtggccga tctcatcgcg tcgcagtctg
     7681 ccgcttcgca ccagctcgcg atgtcgcaga tgcgcggcgg cttcagcgac aagatcagag
     7741 tgctgcgcga aaacctcgtg aacttcgcat cgctcatcga gctcgagctg gatttttcag
     7801 aggaagacgt ggagttcgcc aaccgtgacg acctgaaaaa gctgctgttc acgatccaga
     7861 acctcatcca gcgcctggtc gattccttcg agctcggcaa cgtgatcaag cacggcattc
     7921 ccgtggcgat cgtcggcaag ccgaacgccg ggaaaagcac attgctgaac acgctgctcg
     7981 acgaagaccg cgcgatcgtt tccgaaattc ccggcaccac gcgcgacacg atcgaggacg
     8041 agatcgtcat cgacggcgtg ctgttccgtt tcatcgacac agcaggattg cgcgacacga
     8101 cagacgtgat cgaatccatc ggcgtttccc gttcggtgga gaagatgaaa gcctcttcca
     8161 tcgtcatcta tcttttcgac gtgcgcgaaa tgaccagcgg ggaactgaag cttgcgctgg
     8221 aggaattgaa aaagcacatc catggcgcgc agctcgtgat cgtcgggaac aagatcgata
     8281 aaaagaacat cgcagagatc cagcaggaat tcgccggttt cgacgtgctg ttcatctccg
     8341 cgaaagaaaa gcttttcatc gacgacctgg agcgga
//
LOCUS       DASTSF010000389         3428 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_488605,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000389 DASTSF010000000
VERSION     DASTSF010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3428
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_488605"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            392..646
                     /locus_tag="VFU15_11080"
     CDS             392..646
                     /locus_tag="VFU15_11080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006990409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HEU4718371.1"
                     /translation="MKKGIHPENYRFVVFKDMSNDYMFMTRSTAETKDTITWEDGKEY
                     PLVKLEISNTSHPFYTGKMKLVDTAGRIDKFRSRYEKNKK"
     gene            722..1936
                     /locus_tag="VFU15_11085"
     CDS             722..1936
                     /locus_tag="VFU15_11085"
                     /inference="COORDINATES: protein motif:HMM:TIGR03991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sugar nucleotidyl transferase"
                     /protein_id="HEU4718372.1"
                     /translation="MSPFRLILFDDDFAADFYPLTHTRPVADLRIGIFTIREKWEKRL
                     RSSSTTSTSPWLEKYRWPFEPDAAKDNLWINGRVVPDGELAFEVMNLGEGEAITMGET
                     LIACNCGKSTARIPLNEKDHISGNFAVRDVKAGVVILQRIHDIFMHNGNAIAADEELL
                     KSEDFANELSATNTVIGGNRIYVGKDVTAEACTFNTANGSIILCDGAEVMEGSHLRGP
                     LVIGEHAQVKMGARIYGDTTIGPGCKVGGEITNSVFHSNSNKTHDGYLGNSVIGEWCN
                     IGADSNCSNLKNNYGDIQVYNYSRGGYENTGQQFHGVIMGDHAKCGINTMFNTGTVVG
                     TGANVFGGGFPDKFIPDFSWGGSAGLSDYRFEEFTETVRRVYRRRSLELSAKEAEMLK
                     TVFERTKKFRKS"
     gene            2089..>3428
                     /locus_tag="VFU15_11090"
     CDS             2089..>3428
                     /locus_tag="VFU15_11090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013632947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HEU4718373.1"
                     /translation="MNKHPLFEDIQFNQAEDEETEFIPLLSPEDEEQMNAESVPEVLP
                     VLPLRNTVLFPGVVIPITVGRDKSIRLIKEAYKGNKTIAVVSQKNDAIEDPKAEDLNR
                     IGTVAFIIKMLRMPDGNTTVILQGKRRIVIGEMMQEEPYMLAKVSPLAETFPKKNDSE
                     FAAIIGSVKDTSLQIIRNNPQVPQEAQVAIRNIESNSFLINFISSNMNAEVKEKQSIL
                     EVPDLKERATLVLKLLAKELQMLELKNQIQSKVKTDLDKQQREYFLHQQMKTIQEELG
                     GNPMEQEMNELKEKAKLKKWSKEVAATFSKELSKLERMNPNAAEYSVQMNYLELLLDL
                     PWSEYTEDHFDLAKTEEILDRDHFGLEKVKERILEHLAVLKLKGSNKAPILCLYGPPG
                     VGKTSLGKSIAESLGRKYVRMSLGGLKDESEIRGHRKTYIGAMPGRILQNIRKAG"
BASE COUNT          962 a          862 c          920 g          684 t
ORIGIN      
        1 ccaggaaatg ccgtgtggcg gaaccgatcg tggtgaagac agtgtcaaag acaatggtgt
       61 cgctggaaaa atacagcgtg gcgttgggat cgttgttgag aacgtctttc cggcaaccct
      121 gcccgacgga aacggaaaac agcagaaaga gaatggatgc aaaaaccgca aaacgcattg
      181 ccgcaaaggt agatattcgt ggctaagacg gggaaagcgc cggttttatt gcctttaccg
      241 gtatttttgc cgcgttatca gcctccggga agaaagaata gcgctggaat acagcttttt
      301 gaatgaaaaa acgggggatg aaaccttgca gattacgagg ctcttattat ctttgcgacc
      361 ccgtacggga tacgggcaga aaaataaagc catgaaaaaa ggaatccacc ctgaaaacta
      421 tcgcttcgtt gtgttcaaag acatgtcgaa cgattatatg tttatgacgc gttccaccgc
      481 tgaaacgaaa gacaccatca cgtgggaaga cggaaaggaa tacccgctgg tgaagctgga
      541 aatttccaac acgtcgcatc cgttctatac cggaaaaatg aagctggtgg ataccgccgg
      601 ccgtatcgat aaattccgca gccgttacga gaaaaacaaa aagtaaccgg ccgttaacgt
      661 tcctgatccc tccgaaactt ccggggggat ttgttttttc ggaaatttga aaaaagatcg
      721 catgtctcca ttccggctca ttcttttcga cgacgatttt gcagcggatt tttacccgct
      781 tacgcatacg cggcccgtcg ctgatctgcg catcggcatt ttcacgatcc gggaaaaatg
      841 ggagaaacgc ctgcggtctt cgtccacgac atccacttcg ccctggctgg aaaagtaccg
      901 ctggcctttc gaacctgacg cggcgaaaga caacctctgg atcaacgggc gcgtggttcc
      961 cgacggcgag cttgcgttcg aggtgatgaa cctcggggag ggtgaggcca ttacgatggg
     1021 tgaaacgctc attgcctgca actgcggcaa aagcaccgca cgcattccgc tcaacgaaaa
     1081 agatcacata agcgggaatt ttgccgtgcg tgatgtaaag gccggcgtcg ttattctcca
     1141 gcgcatccac gatatcttca tgcacaacgg gaatgccatt gctgcggatg aagaactgct
     1201 gaagagtgaa gattttgcga acgaactttc agctacgaac acagttatcg gcgggaacag
     1261 gatttacgtg ggaaaggatg taaccgcgga ggcctgcacg ttcaatacgg caaacggttc
     1321 aatcattctc tgcgatggcg ctgaagtgat ggaaggaagc cacctccgtg ggccgctggt
     1381 gatcggcgaa catgcacagg tgaaaatggg cgcccggatt tacggcgata cgacgatcgg
     1441 tcccggctgc aaggtcggcg gtgagatcac caacagtgtc tttcattcga actcgaacaa
     1501 aacgcacgac ggttatctcg gcaacagcgt catcggtgaa tggtgcaaca tcggggccga
     1561 ctcgaattgt tccaatctca agaataacta cggggatatc caggtgtata attattcccg
     1621 cggtggatac gagaataccg gccagcagtt tcatggcgtc atcatgggcg atcatgcgaa
     1681 atgcggcatc aataccatgt tcaatacggg aacggttgtc ggaacgggcg ccaatgtgtt
     1741 cggcggcggt tttcccgata aattcattcc tgatttttcc tggggcggtt ccgccggcct
     1801 ttccgattac cgtttcgagg agttcacgga aaccgtacgg cgcgtttaca ggcgccgttc
     1861 gctcgaactt tccgcgaaag aagccgaaat gctgaaaacc gttttcgagc gcacaaaaaa
     1921 attcaggaaa agctgattta cgacattacg gcaggcattc ctgcatggca tattcattgc
     1981 cacttgtatc ctgcaaacaa tgagatcccg cccgtaatgc ggccccggag agaaagtaaa
     2041 gcacagaaag ccggccggcc cacgggatgt ctgacaaatt gacccactat gaacaagcac
     2101 ccattattcg aagacataca attcaaccag gcggaagacg aggaaacgga atttattccc
     2161 cttctcagcc cggaagatga ggagcagatg aacgcggaat ccgttccgga agttcttcct
     2221 gttctaccgc tgcgcaatac ggtacttttc cccggcgtag ttattcccat cacggtcgga
     2281 agggacaaat ccatccgcct gatcaaggaa gcatataagg gaaacaagac gatcgcggtg
     2341 gtttcgcaga agaatgacgc gatcgaagac ccgaaagcgg aagatctgaa ccgcatcggc
     2401 acggttgcgt tcatcatcaa aatgctgcgc atgccggacg gtaacacgac cgttattctc
     2461 cagggaaaac ggcgcatcgt catcggcgag atgatgcagg aagagccgta catgctggcg
     2521 aaagtttcgc cgctggcgga aacgttcccg aaaaagaacg acagcgagtt cgccgccatc
     2581 atcggttccg tgaaagacac atcgctgcag atcatccgca acaacccgca ggttccgcag
     2641 gaagcacagg tcgcgatacg caatatcgaa agcaacagct tcctcatcaa tttcatttcc
     2701 tcgaacatga acgcggaggt gaaggagaaa cagtccatcc tcgaagttcc cgacctgaaa
     2761 gaaagggcca cgcttgtgct gaagctgctc gcgaaagagc tgcagatgct cgagctgaaa
     2821 aaccagatcc agtcgaaggt gaaaaccgac cttgacaagc agcagcgcga atatttcctg
     2881 caccagcaga tgaaaaccat ccaggaagag ctgggcggaa acccgatgga gcaggagatg
     2941 aatgagctga aggaaaaagc gaagctgaaa aaatggagca aggaggtcgc cgcgacattc
     3001 agcaaggaat tatccaagct cgagcggatg aacccgaatg ctgcggaata ttccgtgcag
     3061 atgaattacc tcgaactgct gctcgatctc ccctggagcg aatacacgga agatcatttc
     3121 gacctcgcaa aaacagagga gatcctcgac cgcgatcatt tcggtcttga aaaagtgaaa
     3181 gaacgtatac tggaacacct ggcggtgctc aagctgaaag gaagcaacaa agcgccgatc
     3241 ctctgcctgt atggtcctcc cggcgtcgga aaaacttctc tcggaaaatc cattgcagaa
     3301 tcgctcggaa gaaaatacgt gcgcatgtcg ctcggcggac tcaaagacga atccgaaata
     3361 cgcggccacc gcaaaacata catcggcgcg atgccgggac ggatattgca gaacatccgg
     3421 aaagcagg
//
LOCUS       DASTSF010000390         2201 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_576704,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000390 DASTSF010000000
VERSION     DASTSF010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2201
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_576704"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2078
                     /locus_tag="VFU15_11095"
     CDS             <1..2078
                     /locus_tag="VFU15_11095"
                     /inference="COORDINATES: protein motif:HMM:NF040138.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HEU4718374.1"
                     /translation="PSNQYGNMSGTSMACPLVAGLCGLMLSLNPSLTPADVRNCLTST
                     AANINSMNPSYIGQLGAGRIDAAAAMNCISGTLNWPPNALFTANVTTVTAGGSVLFTD
                     QSVYNPTTWSWSFPGGTPSSYNGQNPPPIVYNTPGTYNVSLTVTNSHGNDTQTNNNYI
                     TVTAASGCTKVNLPAPAGWTPVNYYTGTAVGQDGWINGVNLYADKEKAMYFDESTQPY
                     TQMVDCWVAFGLAYTTTPNKIVNVKVYDGTSGTPGAQIGTATGVSMGQIMSDVAGNYY
                     TEFSFVNNPITLPASKKIFVSVDLTNLNWSTSHDTLSIVSNTNGQTTPSPIWERQSDN
                     NWYQYTTSGSWNLSASLYIHPFLTGANSVATITASPLTLCAGNQVSFDATGSTYEDTL
                     LWYFPGTTPTISNNVQQDVIYNTAGSYQAILYVVGGGCHLFDSAFVNITVNANPVLSV
                     SLNPSSTTVCSGTPVTMTASGASSYAWSPSTFLNTTTGATVISTPTASISYDLQGTGA
                     NGCTSTTTINLDVDNAPVGMATSLDTVVCVNQPVFFDGSGSSSATGFAWTFPGGSPAT
                     SNASSATVTYATGGNYTATLVVTNACGSDTVTSPVIGVGCMGIDPVAVTDPSAFYNSE
                     MQQLEVQLPVPNDGYTLIIFNSLGQQVKSMRTSDGKVTISTADLAAGMYTLHIVDGMV
                     LHNVKFVKN"
BASE COUNT          523 a          656 c          564 g          458 t
ORIGIN      
        1 ttccttccaa ccaatacggc aacatgtccg gaacttccat ggcctgcccg cttgtggccg
       61 gtctctgcgg acttatgctt tcgctgaatc cttcgctcac gccggcagac gtgcgcaact
      121 gcctgacgtc aactgccgcg aacatcaact cgatgaatcc ttcctacatc ggtcagctcg
      181 gcgccgggcg cattgatgcg gccgctgcaa tgaactgcat cagcggtacg ctcaactggc
      241 cgccgaacgc attgttcacg gctaacgtaa cgacagtgac cgcaggaggt tctgtacttt
      301 tcacggatca gtccgtttac aacccgacaa catggtcctg gtcgttcccg ggcggaacgc
      361 cttccagcta caacgggcag aatcctcctc ccatcgttta caacacgccg ggaacctaca
      421 acgtttcact gaccgttacg aactcacacg gcaacgatac gcaaacgaac aataattaca
      481 tcacagtgac tgcagcaagc ggctgtacga aagtgaatct tcccgctccg gcaggatgga
      541 cacctgtgaa ttattatacg ggaactgctg tagggcagga cggatggatc aacggcgtca
      601 acctttatgc cgataaggaa aaagcgatgt acttcgatga gtcgacgcag ccgtatacac
      661 agatggtgga ttgctgggtc gctttcggac ttgcgtacac cacgactccg aataagatcg
      721 tcaatgtaaa agtgtacgac ggaacttcgg gaacaccggg tgcgcagatc ggcacagcta
      781 ccggcgtttc gatgggacaa atcatgagcg atgtcgcggg taattattac acggaattct
      841 catttgtcaa taacccgatc acgcttccgg cctcgaagaa aattttcgtg agcgtcgatc
      901 ttaccaacct gaactggtcc accagccacg atacgctgag catcgtgagc aacacgaacg
      961 ggcagaccac gccttccccg atatgggaaa gacagagcga caacaactgg taccagtaca
     1021 cgacatcagg atcatggaac ctcagcgctt cgctttacat ccacccgttc ctcaccggcg
     1081 ccaattccgt cgcgacgatc accgcatcgc cgctcacgct gtgtgcagga aaccaggtca
     1141 gcttcgatgc tacgggttcc acctacgaag acacgctgct ctggtatttc ccgggaacaa
     1201 caccgaccat ttccaacaac gtgcagcagg atgtgatcta caacacggca ggaagctacc
     1261 aggcgatcct ctacgtcgtc ggtggcggct gtcacctgtt cgattctgcg ttcgtcaaca
     1321 tcactgtaaa tgcgaatcct gtgctcagcg tgtcgctgaa cccgagcagc accacggtct
     1381 gttccggaac acccgttacc atgacggcct ccggcgcatc atcgtatgca tggagtccgt
     1441 cgacgttcct gaacacaact accggcgcaa ccgtcatatc gacgccgacc gccagcattt
     1501 cgtatgacct gcagggcacc ggcgcaaacg gctgcaccag cacgacaacg atcaacctcg
     1561 acgtggacaa tgcgcccgtc gggatggcga catcgcttga cacggttgtg tgcgtgaacc
     1621 agcccgtctt cttcgacgga tcgggcagct cgagcgccac aggctttgcc tggacgttcc
     1681 ccggaggatc gcccgcaacg tcgaacgcat cttccgcaac ggtgacgtat gcaaccggag
     1741 gtaactacac cgcaacgctt gtcgtgacga acgcctgcgg ctccgacacc gtcacttcac
     1801 cggtgatcgg cgtgggctgc atgggaatcg atcctgttgc agtaacagat ccgtctgcgt
     1861 tctacaacag cgaaatgcag cagctcgaag tgcagctgcc cgttcccaat gacggttata
     1921 cgctgatcat cttcaacagc ctcggccagc aggtgaaatc gatgcgcact tccgacggaa
     1981 aagtgacgat cagcacggcc gacctcgcgg cgggaatgta cacgctgcac attgtggacg
     2041 gaatggtgct ccacaacgtg aagtttgtga agaactgaaa caacactttc tgaaatgaaa
     2101 aaagtcctgc agaaatgcgg gactttttct atgtggtgaa aaatccggaa ccgggctttg
     2161 tgttttcgtg tttctgcggt ttcttttatt cgccgattcc a
//
LOCUS       DASTSF010000391         4517 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1448143,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000391 DASTSF010000000
VERSION     DASTSF010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4517
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1448143"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..117)
                     /locus_tag="VFU15_11100"
     CDS             complement(<1..117)
                     /locus_tag="VFU15_11100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018965455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP synthase"
                     /protein_id="HEU4718375.1"
                     /translation="MNFPRAAITGVECWLPEDRLTNADIEKLVDTSNEWILER"
     gene            259..1824
                     /locus_tag="VFU15_11105"
     CDS             259..1824
                     /locus_tag="VFU15_11105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718376.1"
                     /translation="MPKEVSDHLFRLIGRMSKSEKRFFKLMAARQKGGGEKFVVLFDR
                     LEKMKTYDEELLLKREPSLSRKQLPNLKSFLYSYLLRSLRLCNSSGSADVRLSDYCCE
                     ARILYDKCLYRDSLRMIERARKFAHTHGRTGPLMDLLDLEKEVIRHTITADHAQKVDE
                     SVSDTGEVLEQLNRTTQFSNLLIRLNAFYVQSGFIRNRKDLYSVQRFFRKNLPAYEES
                     RLGFPEKLYLYSAFTGYYFFIQDFRNGYRYSKRSVDLFEKFPGQIYNYPEFYIRALNS
                     QLVVLNKFRRLEEFEAVHRQLIALKRNGAFDKTENINLNLFRAIYVHEINRHFMMGEF
                     RSGVRVVAKLEKELNRFIPLLDHHSLLLFYYKIACLYFGADQYRSALHWLSRIIGEKD
                     ISVREDLHAFARILALICHFELGNDRLAEAHVKSLYRYLLRKGDLSAYHREILAFIRK
                     LHRNLSPRELNLLFHRLKDKMSSLSRQRFERRAFFYFDIITWLESKISNRTVEQIIKE
                     KVKAADRKLLAKK"
     gene            1888..2451
                     /locus_tag="VFU15_11110"
     CDS             1888..2451
                     /locus_tag="VFU15_11110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718377.1"
                     /translation="MKRIIPVFFFLCACLKAEATGMDDSTKVHPPFFVYAEGLGPMMY
                     YSLDGEFRFFTSHDSNRAVYARIGFENIPDRAVRWGSGLSAGIQVDAGQKKLTRNVGA
                     GYVCWFDSHNYRASGEFTTYCDKPHCEPSLRGGVYLSIGVRYHIDDLFFAGFAFTPEL
                     LGNSYAWEVRPSMALSGGVMLDGFRKK"
     gene            complement(2460..2831)
                     /locus_tag="VFU15_11115"
     CDS             complement(2460..2831)
                     /locus_tag="VFU15_11115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718378.1"
                     /translation="MADGSLPEKPPSSLDSSDLVQLLLPSAVSILENDFRTCGIDPGM
                     EGKLFPSVFGLRDFISTLMEKQAAPGSEAFFRLLYRNDLPEKKAMDALAAENGQRDSE
                     KIAELIIIRALQRAFYRMKYG"
     gene            complement(2838..3164)
                     /locus_tag="VFU15_11120"
     CDS             complement(2838..3164)
                     /locus_tag="VFU15_11120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013928646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HEU4718379.1"
                     /translation="MSDTPVYHKHIFICINQRAPGSPRRSCGEAHGMEIVDAFKKKLK
                     EKNLPFPVRAQKAGCLDICDFGQTVVVYPEGVFYVGVEISDVDEIIEEHIRNDRPVER
                     LRLKRK"
     gene            3261..4172
                     /locus_tag="VFU15_11125"
     CDS             3261..4172
                     /locus_tag="VFU15_11125"
                     /inference="COORDINATES: protein motif:HMM:TIGR01777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HEU4718380.1"
                     /translation="MKILISGASGLIGRHLTETLVQQGHEVRHLVRNGKTGEKVKQFP
                     WDPAKNMISEEAIPGTDIIINLSGAPVAGKRWTTARKEEIVSSRVNALNTLREGVKKS
                     GNRKVRLISVSGVNYYGIHPGNHVFYEKENPGDDFLGRCCVEWENAALKFSQEGIPVT
                     ILRVGIVLAKNGGALPKIAAPVKWFAGAPFGSGKQWMPWIHIGDLCRMFLFLAEHPEH
                     TGIFNAVAPEAVNNSQFTLQLARTLHRPLVLPAVPGFLLRLVLGEMADVLLNGNLVSG
                     EKIRDAGFTFGFPFLENALKDLYSDEK"
     gene            complement(4178..>4517)
                     /locus_tag="VFU15_11130"
     CDS             complement(4178..>4517)
                     /locus_tag="VFU15_11130"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type B sorting domain-containing protein"
                     /protein_id="HEU4718381.1"
                     /translation="IVYNQYGCSDTAVIPIEVRPEFRFFIPNTFTPNGDGLNDIFMPA
                     IMGVDNYHFMIFDRWGNLIFETSDTFQGWDGRYKGNKCQEDVYVWKIDFTNVVDESNQ
                     ELIGHVNLIR"
BASE COUNT         1059 a         1172 c         1228 g         1058 t
ORIGIN      
        1 tcgctcaagg atccattcgt tgctcgtatc cacgagcttc tcgatatctg cattcgtcag
       61 ccggtcttcg ggaagccagc attccacgcc tgttatagca gctctgggaa aattcatctg
      121 gtttgattta gggaatacga atatacgcgg aaaactcccc cgggcggctt ccaaaattac
      181 gatgtcaacc gcagctcaaa attcaaaagt agccctgaaa gccctgtaaa ttgggtattt
      241 ttacacaaaa ataatacgat gcccaaagag gtttccgacc accttttccg gctcatcggc
      301 aggatgagca aaagcgaaaa aaggttcttc aagctgatgg cggcgcgaca gaaaggcggt
      361 ggcgaaaagt tcgtcgtgct gttcgatcgc ctggaaaaaa tgaaaacgta cgacgaagaa
      421 ctgctgctga aacgcgagcc ttccctttcc aggaagcagc tgcccaacct gaaatcgttc
      481 ctctattcct acctgctccg ctcgctccgg ctctgcaatt cgtccggttc cgccgatgtg
      541 cgtctttcgg attactgctg cgaagcccgc attctctacg acaagtgcct ctaccgcgat
      601 tccctgcgca tgatcgagcg cgcgcggaaa tttgcgcaca cgcatggccg cacgggtccg
      661 ctcatggacc tgctcgatct cgaaaaagaa gtgatccgcc acaccattac cgccgatcac
      721 gcacagaaag tggatgaatc ggtgagcgat acaggcgaag tgctcgaaca gctgaacagg
      781 acgacgcagt tcagcaacct gctcatccgc ctcaacgcgt tttatgtgca atcgggattc
      841 atacgcaacc ggaaagatct ttacagcgtg caacggtttt tccggaaaaa tctcccggcc
      901 tacgaagaaa gcaggctcgg tttcccggaa aagctttacc tgtacagcgc atttacggga
      961 tattattttt tcatccagga ttttcgcaac ggttaccgtt attcgaaacg atcggtcgac
     1021 ctgttcgaaa aattcccggg gcagatctac aattacccgg agttctacat ccgcgcgctc
     1081 aacagccagc tcgtcgtgct gaacaagttc cggcggctgg aagaattcga agcggtccac
     1141 cggcagctta tcgcgctgaa gaggaacgga gcgttcgaca aaacggaaaa catcaacctc
     1201 aatcttttcc gcgcgattta cgtgcacgag atcaacaggc attttatgat gggagaattt
     1261 cgttcgggcg tgcgcgtggt ggcgaagctg gagaaagagc tcaaccggtt catcccgctg
     1321 ctcgatcacc attcgctgct gctgttctac tacaagatcg cctgcctgta tttcggtgca
     1381 gaccagtacc ggagcgcgct gcactggctg tcgcgcatca tcggggaaaa agatatttcc
     1441 gtgcgcgagg acctgcacgc atttgcgcgc atacttgcgc tcatctgcca tttcgagctc
     1501 ggcaacgacc ggctggcgga agcgcacgtg aaatcgcttt accgttacct gctgcgcaag
     1561 ggagatcttt ccgcgtacca ccgcgagatc ctcgcattca tccgcaagct ccaccggaac
     1621 ctttccccgc gcgaactgaa cctgcttttc caccggctga aagacaagat gagcagcctc
     1681 tcgcggcagc gtttcgaacg tcgcgcattc ttttatttcg atatcatcac gtggctggaa
     1741 agcaagatca gcaaccggac ggtggagcag atcatcaagg aaaaagtgaa agcggcagac
     1801 cggaagctgt tagcgaaaaa atgaaaacta ttccggtgac gtaatgacgc gttcaccgcg
     1861 cagagtaatt ttgaacgaac gaaaaaaatg aaaaggatca ttcccgtttt ctttttcctt
     1921 tgcgcatgcc tgaaggcaga ggcgacgggc atggacgatt ccacgaaagt tcatccgccg
     1981 ttttttgtat acgcggaagg gttaggtccc atgatgtatt attcgctcga cggcgaattc
     2041 cgttttttta cttcgcatga tagtaatcgc gctgtttatg cacggatcgg ttttgaaaat
     2101 atcccggatc gtgccgtcag gtggggaagc ggcctgtcgg ccggtatcca ggtggatgcc
     2161 gggcagaaaa agctgacgag gaacgtgggc gccgggtatg tctgctggtt cgattcgcac
     2221 aattaccggg ccagcggaga attcacgacg tactgcgaca aaccgcattg cgagccttcg
     2281 cttcgcgggg gcgtgtatct ttcgatcggt gtgcgttacc acatcgatga tctttttttt
     2341 gccgggtttg cctttacgcc cgagctgctg ggaaacagct atgcatggga agtgcgtcca
     2401 tccatggctt taagcggtgg tgtgatgctg gacggcttcc gcaaaaaata attttctcct
     2461 cagccgtatt tcatccggta gaatgcgcgc tgaagcgcac ggatgataat gagctccgcg
     2521 atcttttcgc tgtcgcgctg cccgttttcc gccgcaagcg cgtccatcgc ttttttttcg
     2581 ggaagatcgt tcctgtacag caggcggaag aaggcttccg atccgggagc tgcctgtttt
     2641 tccatcagcg tcgaaataaa atcacgcagc ccgaaaacag aaggaaataa ttttccttcc
     2701 attcccggat cgatgccgca cgtacggaaa tcattttcaa gaatgctgac ggcggaaggc
     2761 agcaggagct gaacgaggtc cgagctgtcg agagacgaag gcggtttttc cgggagagat
     2821 ccgtcagcca tggaaattca ttttcttttc aggcgcaggc gttcgacggg acggtcgttc
     2881 cggatgtgtt cttcgatgat ctcgtcgacg tcgctgatct ccacgccgac ataaaaaacg
     2941 ccttcgggat aaacgacaac cgtctggccg aaatcacaga tgtcgaggca gccggctttc
     3001 tgcgcgcgca cggggaacgg aagatttttt tctttcagct ttttcttgaa cgcgtccacg
     3061 atctccatac catgcgcttc tccgcagctt ctccgcggcg agccgggtgc gcgctggttg
     3121 atgcagatga aaatatgctt gtggtaaaca ggtgtgtcgc tcatggatcc gttatgaact
     3181 gcaatgataa gaaacggtga caaaaattct caatagggta aaacaagaat agtaatttgc
     3241 aggctcaatt tatccgggtt gtgaaaattc ttatttcagg agcgtcagga ttgatcggcc
     3301 gccatcttac ggaaacgctg gtgcagcagg gccatgaagt gcggcatctt gtgcggaacg
     3361 gaaaaacagg agaaaaggtg aagcagtttc cctgggaccc ggcaaagaac atgatcagtg
     3421 aagaagcgat tcccgggacg gacattatca tcaatctttc gggggcgcct gttgccggca
     3481 aacggtggac cacagcgcgg aaggaagaga ttgtttccag tcgtgtgaat gcgctgaaca
     3541 cattgcggga aggcgtgaag aaaagcggga acaggaaggt ccgcctcatc tccgtttccg
     3601 gtgtcaacta ttacggaatt catccgggga atcacgtgtt ttacgaaaag gaaaatccgg
     3661 gtgatgattt cctcgggcgt tgctgcgtgg aatgggaaaa tgcggcgctg aaattttcgc
     3721 aggaaggaat tcccgtgacg atcctgcgtg tgggcattgt gctggcgaag aacgggggag
     3781 cgcttccaaa gatcgcggcg ccggtgaaat ggtttgccgg tgcgccgttc ggcagcggga
     3841 agcaatggat gccgtggatc cacatcggcg atctctgccg catgttttta tttctcgccg
     3901 aacatccgga gcacacgggc atcttcaacg cggtggcgcc ggaagctgtg aacaacagcc
     3961 agtttaccct gcagctcgcc aggacgctgc accgcccgct ggtccttccc gccgtgccgg
     4021 gtttcctgct tcgtcttgtg ctcggcgaaa tggcggatgt gctgctgaac gggaacctcg
     4081 tttcgggaga aaagatccgc gacgccgggt ttacgttcgg gtttccgttt cttgaaaatg
     4141 cgttgaagga cctgtattcc gatgaaaaat aatccgctca gcggatgagg ttcacgtgcc
     4201 cgatcagctc ctggtttgat tcgtccacga cattcgtgaa atcaattttc cacacgtaaa
     4261 catcttcctg gcatttgttg cccttgtatc ttccgtccca gccctggaac gtgtcgctgg
     4321 tttcgaagat gagatttccc cagcggtcga agatcatgaa atgataattg tccacgccca
     4381 tgatcgccgg cataaaaatg tcgttcagcc cgtcgccgtt tggcgtgaac gtattcggaa
     4441 tgaagaagcg gaattccggc cgtacttcga tcgggatcac cgcggtgtcc gaacatccgt
     4501 attgattgta cacgatc
//
LOCUS       DASTSF010000392         3044 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_28212,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000392 DASTSF010000000
VERSION     DASTSF010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3044
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28212"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(99..581)
                     /gene="ribH"
                     /locus_tag="VFU15_11135"
     CDS             complement(99..581)
                     /gene="ribH"
                     /locus_tag="VFU15_11135"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEU4718382.1"
                     /translation="MATALKNLSQHDPEKVPSGKGKKIAVVVSEWNSEVTFGLLEGAV
                     ATLIKYGVKKKDIAVSYVPGAFELTLGCLRAGSPGKINAVIAIGCVVRGDTPHFEFIC
                     QGVTTGITQLNISLAKPVIFGVLTTNTLQQALERSGGKHGNKGVEAAVTALKMCGKQN
                     "
     gene            complement(638..1324)
                     /locus_tag="VFU15_11140"
     CDS             complement(638..1324)
                     /locus_tag="VFU15_11140"
                     /inference="COORDINATES: protein motif:HMM:NF024574.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU4718383.1"
                     /translation="MAKAEPKTKEQVIDIEGAFDRAEHYIEENRKSLLFIVGGIVLLV
                     GLYFGWKYFYIQPRTEEANSKMYRAEMLFAKDSFNLAINGHGDTTGFADIVEDYGGTS
                     AGNISRYYLGICYMRTNQYDKAIDVLKDFDSNDEFVGSMALGLIGDAYMEKQQTDDAI
                     EYYLKAAKRKPNKFTSPVFLKKAAFAYEDKGNKDEALKLFEQIKVEFPESQEATQVDK
                     YIARNGGEVK"
     gene            1427..2533
                     /gene="recF"
                     /locus_tag="VFU15_11145"
     CDS             1427..2533
                     /gene="recF"
                     /locus_tag="VFU15_11145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013547040.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HEU4718384.1"
                     /translation="MILRRLSLLNFRNYEEVSVDCSERINCFTGLNGSGKTNLLDAIH
                     YLSLCKSFLNPIDSQNIRFDSPFFMIQGMFESAEGREENILCSVKRGQRKVFRRNGKD
                     YERLSDHIGLCPLVAVSPGDGTLVTGGSEERRRFMDTVISQYNHSYLDHLIAYNRVLS
                     QRNALLKQMSEGKSADDGTREILDLQLEQNGQPVFIARKAFIRELLPFFSRFYESLSG
                     GNERVELVSESKMNEKPLSQLLNESLQKDTILGHTTCGIHKDDLDFRINGHSLKRSGS
                     QGQQKTFIIALKLAEYIFLDRAGGKKPMLLLDDVHDKLDEDRVVRLMEIVTSKDFGQL
                     FITDTGAGRMNELFRERGLPYKMFRVEAGKITGQ"
     gene            2668..>3044
                     /locus_tag="VFU15_11150"
     CDS             2668..>3044
                     /locus_tag="VFU15_11150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718385.1"
                     /translation="MLKKYILIASVAFTCALNAQTPTVYPGMPQKTTDPKQQKPVYSG
                     PSAAGDMKRDNGDFAGAIVEYNKEITKIDADAQRIAKLKTDYEKMSEFDRMNQNQDEA
                     TKNYPDWAKLYYGRAMANIGLGK"
BASE COUNT          721 a          824 c          757 g          742 t
ORIGIN      
        1 accccctggt cccaaaccag gtgcgctacc aactgcgcta caccccgaaa acaatatgta
       61 aaaagaacag gttccctttt ttcaggccgc taaagtagtc aattctgctt accgcacatt
      121 ttcagcgcgg tcacggccgc ctccacgcct ttgtttccat gttttccgcc cgagcgttca
      181 agggcctgtt gcagtgtatt ggtggtcagc acgccgaaaa taacaggctt cgcaagcgaa
      241 atgttcagct gcgtgatgcc ggtcgttaca ccctggcaga tgaattcgaa atgcggcgta
      301 tctccgcgca caacacaacc gatcgcgata acggcgttta tttttcctgg cgaaccggcc
      361 cggagacagc ccagcgtcag ctcgaatgcg cccggcacgt agctcaccgc gatgtctttc
      421 tttttgacgc cgtatttgat gagcgtggcg acagcgcctt ccagcagccc gaacgtcact
      481 tcgctgttcc attccgaaac gacaacggct atttttttgc ctttgcccga cggaactttt
      541 tccgggtcgt gctgcgaaag atttttaagt gctgtggcca tgtcaaaaaa aatcccgccc
      601 ggtgctccgg acgggaaaaa tgagttttcc ggagaattta ttttacctcg ccgccgtttc
      661 tggcaatgta tttatccacc tgcgtggctt cctgcgattc cgggaattcc accttgatct
      721 gctcgaaaag cttcagcgct tcgtccttgt tccctttgtc ttcgtaagca aatgccgctt
      781 tcttgaggaa aaccggtgaa gtgaatttat tcggtttcct tttcgccgcc ttgaggtaat
      841 actcgatcgc gtcgtcggtt tgctgttttt ccatgtaagc atcgcctatc agtccgagcg
      901 ccatgctgcc gacgaattcg tcgttcgaat caaaatcctt cagcacatcg atcgccttgt
      961 catactggtt cgtgcgcatg tagcagatgc cgaggtaata acgggaaatg ttcccggcag
     1021 acgttccgcc gtaatcttcc acgatgtctg cgaagcctgt cgtatcgcca tgaccgttga
     1081 tggccaggtt gaacgaatct tttgcgaaaa gcatttcagc gcggtacatt ttcgagttcg
     1141 cctcttccgt acgcggctgg atgtaaaaat atttccaccc gaaataaagg cccaccagca
     1201 gcacgatccc tcccacgatg aataaaaggc tcttccggtt ttcctcgata taatgctcgg
     1261 cgcgatcgaa cgcaccttcg atatcaataa cttgttcttt cgttttgggt tctgcttttg
     1321 ccatgacaaa aaagccggta tctttttacc ggtcagggcg caaaaataat ctttttttca
     1381 actggctcgg aaatcgcccc gctttttggc aattttgcct tctcagatga tcctccggcg
     1441 tctttccctg ctgaatttca gaaattatga agaagtctct gtcgactgct cggaaaggat
     1501 caactgtttc acggggctga acgggtcggg aaaaacgaat ctcctggacg ctatccacta
     1561 cctctcgctc tgcaaaagct tcctgaatcc catcgacagc cagaacattc ggttcgactc
     1621 gccttttttc atgatccagg gaatgttcga atccgccgaa ggccgcgagg aaaatatcct
     1681 ctgctcggtg aaacggggac agcggaaagt attccggcgc aatggaaaag actacgaacg
     1741 gctttcggat catatcgggc tttgcccgct ggtggcggtt tctcccggcg acgggacgct
     1801 tgtaacgggc ggaagcgaag agcgccgtcg cttcatggat acggtcattt cccagtataa
     1861 tcattcctac ctcgatcacc tgatcgcata caaccgcgtg ctttcccagc ggaatgcgct
     1921 gctgaagcag atgtcggaag ggaaatcggc cgacgacggg acacgcgaga tcctcgacct
     1981 gcagctggag cagaacgggc agccggtttt cattgcacgc aaagcgttta tccgcgagct
     2041 gctcccgttc ttcagccggt tctacgaatc gctcagcggc gggaacgaac gcgtggagct
     2101 ggtgtcggaa tcgaaaatga atgaaaaacc tttgtcgcag ctgctgaacg aaagccttca
     2161 gaaagacaca atccttggtc ataccacgtg cggcattcat aaagatgatc tcgatttccg
     2221 catcaacggg cattcgctga aaaggagcgg atcgcaggga cagcagaaaa cgttcatcat
     2281 cgcgctcaag ctggcggaat atatttttct cgaccgtgcc ggtggcaaaa agcccatgct
     2341 gctgctggat gatgtgcacg acaagctcga tgaagaccgt gttgtccgcc tgatggagat
     2401 cgttacgtcg aaggatttcg ggcagctgtt cattaccgac accggggccg gaagaatgaa
     2461 cgagctcttc cgtgaacgcg gtcttcccta caaaatgttc cgcgtggaag cgggaaaaat
     2521 aaccgggcag tgaatgcgcc gtgtgttgtt ccgggtcttt tttggcatga ttgtttcata
     2581 atcttcctgc agtaagccag ataacaccct gatttttcag cagaaaggct tttttgaata
     2641 cttttgtcca accaaatacc caatggcatg ttaaaaaagt acattctcat cgcatcagtt
     2701 gcctttacct gcgcccttaa tgcccagaca ccgaccgttt acccggggat gccccagaaa
     2761 acgacggatc cgaagcagca gaagcccgtc tattccgggc cttccgccgc gggcgacatg
     2821 aagcgcgaca atggtgattt tgcaggtgcg attgttgaat acaataagga gatcaccaag
     2881 atcgatgccg atgcacagcg catcgcgaag ctcaaaaccg attacgaaaa aatgtccgag
     2941 ttcgacagga tgaaccagaa ccaggacgag gcgaccaaaa actacccgga ctgggcaaag
     3001 ctgtattacg gacgtgccat ggccaacatc ggtctcggca aaaa
//
LOCUS       DASTSF010000393         5098 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1549892,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000393 DASTSF010000000
VERSION     DASTSF010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5098
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1549892"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..450)
                     /locus_tag="VFU15_11155"
     CDS             complement(<1..450)
                     /locus_tag="VFU15_11155"
                     /EC_number="1.13.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol ring-cleavage dioxygenase"
                     /protein_id="HEU4718386.1"
                     /translation="MELKSFSTISESFSPTGRMPVIFTSHGNPMEALSDLNATPFFSS
                     LGEVSRRIRAEHTINAVLIVSAHWLTRGTYVNVSEHPKTVHDYYGFPPEFYEIQYPAP
                     GSPSFARETAKLIPGVKETTEWGLDHGSWPVLRHYFPGADVPVFEMSI"
     gene            complement(527..1003)
                     /locus_tag="VFU15_11160"
     CDS             complement(527..1003)
                     /locus_tag="VFU15_11160"
                     /inference="COORDINATES: protein motif:HMM:NF014168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEU4718387.1"
                     /translation="MYRYTKIYHLQKYTKYPDICTVIKTAPIMKAIVLTGRILFSLIF
                     LLSVMGHFSQPSIAYAASAGVPAASFLVPLSGVIAAAGALSIILGYKAKWGAWLIVLF
                     LIPVTFMMHAFWKATDPVAQQMQMVMFMKNIALLGAALLITYFGSGPLSLDSRLQK"
     gene            1061..1432
                     /locus_tag="VFU15_11165"
     CDS             1061..1432
                     /locus_tag="VFU15_11165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015812158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU4718388.1"
                     /translation="MTPVRKTGLKAEDAGECKKSLLPVRDALYVLSGKWKLPIIVALA
                     HGHKRFKELQREIKGITARMLSKELRELEMNELVSRTVYDTTPVTVEYELTKYGETLD
                     TVIVALRDWGLRHRKKIKARK"
     gene            1440..2009
                     /locus_tag="VFU15_11170"
     CDS             1440..2009
                     /locus_tag="VFU15_11170"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008405622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEU4718389.1"
                     /translation="MLKLKMIITSTRPGRKGPAVAQPFFDIAAVHPGFETELVDLAEM
                     DLPLLDEPHHPRLQQYTKEHTKRWSALIDGADAFIFVLPEYNFSFPATVKNALDYLAK
                     EWSYKPVGFISYGGMSGGLRAVDVLLPVIRAFKMVALVEGVHIAGFPKHFNAEGKFIA
                     DETMQKAMHTMLAELQRWAEALKPLREKK"
     gene            2068..2838
                     /locus_tag="VFU15_11175"
     CDS             2068..2838
                     /locus_tag="VFU15_11175"
                     /EC_number="1.1.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002695705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003858 - 3-hydroxybutyrate dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HEU4718390.1"
                     /translation="MTKNVLITGSTSGIGLGIAREFAKTGCNISFNGLEKDGPEIAEA
                     VGKEFGVKTVFSGANLLDADAIQKFMGESEKKLGGIDVLVNNAGMQFVSEIENFPVEK
                     WNMIIGLNLSAAFHTTRLAIPMMKKKKWGRVINIASAHGLVASEFKSAYVAAKHGLVG
                     LTKVTALEGAPFGITANAVCPGYVDTPLVRGQIADQAKTHHMSEEEVIAKVMLVKHAV
                     KKFVKTESLGALCVFLASDHAELITGTAIPVDGGWNAQ"
     gene            3066..3350
                     /locus_tag="VFU15_11180"
     CDS             3066..3350
                     /locus_tag="VFU15_11180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718391.1"
                     /translation="MNELFSKVVILYNLAGSNLAGSNLAGSNLAGSNLAGWYPGRQPC
                     RLPDDLFLVFDQISYSKSLILHELVRVVVILYNLAGSNLAGWYPADNLAG"
     gene            3607..4644
                     /locus_tag="VFU15_11185"
     CDS             3607..4644
                     /locus_tag="VFU15_11185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002693891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEU4718392.1"
                     /translation="MNPRIRFDKKLTAMALQGGGAHGAFTWGVLDRLLEVDELVAEGI
                     CGTSAGAVNAVVLAYGLHMGGPGKAKELLETLWKKIGQYGSYLFKPSSWDRMMNFGDI
                     YNSPGYLFFNSISQSLSPYQLNPFNYNPLRDILLDLIDFKELRQYSTKKLFVCATNVK
                     TNRARIFHNKEITVDAVLASACLPFLFQAVEIDGEYYWDGGYMGNPPLTPLISETKTR
                     DIVLVKINSINISKLPTSARDIAERVNEISFNSSLINEMQMVHYRNELIRRGVSLPEQ
                     NREIFVHCISGYNSLDHLSYSSKLNTSWEFLQHLKEEGRKTADAWLENEYDLVGVRST
                     FDVEEHFLKRY"
     gene            complement(4669..>5098)
                     /locus_tag="VFU15_11190"
     CDS             complement(4669..>5098)
                     /locus_tag="VFU15_11190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718393.1"
                     /translation="LAAAGFHYLSRSFFSLRMMKQNVFEASVTSRNGEERSFPGITSI
                     EFYYIILPGYRGSRKVLCYVNFCDSSGNSRLTLKEEYGISSLSNETNPRPPGNFQLYV
                     PGKKMADAVYYGPVREIEQAFFHFRRNERNEKSSEQVTAN"
BASE COUNT         1309 a         1240 c         1379 g         1170 t
ORIGIN      
        1 gatgctcatt tcgaaaacag gtacgtccgc accgggaaaa taatgacgca gcaccggcca
       61 tgagccgtga tcgagtcccc attccgttgt ttccttcacg ccgggaataa gcttcgcggt
      121 ttccctcgca aacgagggag aacccggagc aggatattgg atctcgtaaa attcaggcgg
      181 gaaaccgtaa tagtcgtgga ccgttttcgg gtgctcgctc acgttgacgt atgtgccgcg
      241 ggtgagccag tgcgcggaaa cgatcagcac ggcattgatc gtatgctccg ccctgatcct
      301 gcggcttact tctcccaggc tggagaaaaa aggtgttgcg ttcagatccg aaagcgcttc
      361 catcgggttg ccgtgcgagg tgaagatgac gggcattctc ccggtcgggg aaaacgactc
      421 gctgatcgtt gagaatgatt tcagttccat gttgaatgaa caaagttcgg gttttaggcg
      481 aaccgcttta ccacaagatg agtatcactg tttacagatg aacggatcat ttttgcaagc
      541 gggaatcgag gctgagcggc ccggatccga aatacgtgat aagcagcgca gcgccaagaa
      601 gagcgatgtt tttcatgaac atgaccattt gcatttgctg tgccaccggg tcggtcgctt
      661 tccagaacgc gtgcatcatg aatgtaaccg ggatgaggaa caggacgatg agccaggcgc
      721 cccattttgc cttgtaaccc agaatgatgc tcagtgcgcc ggccgctgcg atcaccccgg
      781 acaacggcac gaggaaggaa gccgcgggaa cgccggcaga agcagcatac gcgatggaag
      841 gctgcgaaaa atggcccatg acggacagta gaaaaatgag cgagaaaagg atacgccctg
      901 tcaatacgat tgctttcatg attggtgcag ttttgattac agtgcaaatg tccgggtatt
      961 tggtatactt ttgtaagtga tatatttttg tataccggta tataaaagta taccgggttg
     1021 aatccggttg aaaagcatcg tagttttaaa tcctgaaaaa atgacaccag tacggaagac
     1081 cggtctgaaa gcggaggacg ccggggaatg caaaaaaagc ctcctccctg tgcgtgacgc
     1141 gctttatgtg ctcagcggaa agtggaagct gcccattatc gtggcgctgg cgcacgggca
     1201 caaacgattc aaagagctgc agcgcgagat caaaggcatc acggcgcgca tgctttcgaa
     1261 agagctgcgc gagctggaaa tgaacgagct cgtttcccgc acggtatacg ataccacacc
     1321 tgtcaccgtg gaatacgagc tgacgaaata cggggaaacg ctggatacgg tgatcgtcgc
     1381 gctccgcgac tggggactgc gccaccggaa aaaaataaaa gcaagaaaat aaaacgttta
     1441 tgctaaaact gaaaatgatc atcaccagca cgcggcccgg aagaaaaggc ccggcggtgg
     1501 cgcaaccttt ttttgacata gccgcagtac acccgggatt tgaaacggag ctggtcgatc
     1561 ttgcggagat ggatcttccg ttgctggatg aaccgcatca cccgcgcctg cagcaataca
     1621 cgaaagaaca taccaaacgg tggagtgcgc tcatcgacgg tgcagacgcg ttcatatttg
     1681 tccttcctga atataatttc agctttcccg ccacggtcaa gaacgcactc gattacctcg
     1741 cgaaggaatg gagctacaaa ccggtcggtt tcatcagcta cggcggcatg tcgggcggac
     1801 tgcgtgccgt agatgtgctg cttcctgtga tccgcgcttt taaaatggtc gcgctcgtgg
     1861 aaggggtgca cattgcgggt ttcccgaaac atttcaatgc ggaaggaaag ttcatcgccg
     1921 acgaaacgat gcagaaagcg atgcacacga tgcttgcgga actgcagcga tgggccgaag
     1981 cgctgaagcc gctgcgcgaa aagaaataaa attctttctt attctgccgg taaaatccgg
     2041 ctcgcggcag ctgagtatat ttgccgcatg acaaaaaatg ttcttatcac aggaagcacc
     2101 agcgggatcg ggctgggcat tgcacgtgaa ttcgcaaaaa cgggctgcaa tatttcattc
     2161 aacggacttg aaaaggacgg acctgaaatt gcagaagcgg tagggaaaga attcggcgtg
     2221 aaaactgttt tttccggggc caaccttctc gatgcggatg caatacaaaa attcatgggg
     2281 gagtcggaga aaaagctcgg cggcatcgat gtgcttgtga acaacgcggg catgcagttc
     2341 gtttctgaaa tcgaaaattt cccggtggaa aaatggaaca tgatcatcgg gctgaacctt
     2401 tctgcagcct tccatacaac acggctcgcc attcccatga tgaaaaagaa aaaatgggga
     2461 cgcgtgatca acatcgcatc tgcgcacggg ctcgtcgctt ccgaattcaa gtcggcctac
     2521 gtggcagcca agcacggtct ggtcggtctc acgaaagtca ccgcgctcga aggcgctccg
     2581 ttcggcatca cggcaaatgc tgtttgtccg ggttacgtcg atacgccgct ggtgcgcggg
     2641 cagatcgcag accaggcgaa aacgcatcac atgagcgagg aggaagtgat cgccaaggtg
     2701 atgcttgtga aacacgcggt gaaaaaattc gtgaagacgg aatcgctggg cgcgctgtgt
     2761 gtttttcttg cgtcggacca cgcagagctg atcaccggaa cggcgatacc ggtcgacggc
     2821 ggctggaacg cgcagtaacg gatcgggttc aggaaaggtc gaaaatattt tctgagacgg
     2881 atcccgatac cggatcgaaa cggcctggat tcatttgcaa acggtaaaaa cgtttttacc
     2941 tgtttgtaag cggttgaaat cgtttactgg tattttaaac ggacaagctt acaagttgtg
     3001 accttgaagg ttgagggaac gactttttta ttttcgacgt gataagacgt aataagtcct
     3061 tcgttttgaa tgaattattt tcgaaagttg taatactata caaccttgca ggttctaacc
     3121 ttgcaggttc taaccttgca ggttctaacc ttgcaggttc taaccttgca ggttggtacc
     3181 ccggcagaca accttgcagg ttgccggacg acctctttct tgtttttgac cagataagtt
     3241 atagtaaatc cctgattttg catgaattag ttcgtgtagt tgtaatatta tacaaccttg
     3301 caggttctaa ccttgcaggt tggtacccgg cagacaacct tgcaggttga cacccggaag
     3361 caagcccatc atccggaaac gcggtcggtt ttttacaagc ccattcgaaa tcgcatgaat
     3421 atcattgata gtcatgatcc acggacggag ccctttactt cgatttataa acaaagcttc
     3481 cggccaggcg ggatgaacgc tcgttgctgg cggatgaagc gaaggattat accgttgagt
     3541 ttccccgctt gtcaacgctg cattttaccg gcacggtgaa aaaactattt agctaaattc
     3601 acgccagtga atcccaggat acgttttgac aagaagctga cggcgatggc attgcagggc
     3661 ggaggagcgc acggcgcgtt cacgtggggc gtgctcgacc ggctgctgga agtggacgag
     3721 ctggtggccg aagggatatg cggaacgagc gctggagcgg tgaacgccgt ggtgctggcc
     3781 tacgggctgc acatgggcgg gcccgggaaa gcgaaagagc tgctggaaac gctctggaaa
     3841 aaaatcgggc agtacggcag ctatcttttt aaaccttctt cctgggaccg catgatgaac
     3901 ttcggcgata tctacaattc tcccggatat ctttttttca attcgatctc ccagtcactt
     3961 tctccttacc agctgaaccc gttcaattac aacccgctcc gggacatcct gctcgacctc
     4021 atcgatttca aagaacttcg ccagtacagc acgaaaaagc tcttcgtctg tgcgacgaac
     4081 gtaaagacga accgcgcgcg gatctttcac aataaagaaa ttactgtcga cgcggtgctt
     4141 gcttccgcct gtcttccttt tcttttccag gctgtggaga tagacggtga gtattactgg
     4201 gacgggggct atatgggcaa tccaccgctg acgccattga tctccgagac aaaaacgcgc
     4261 gacatcgtgc tggtgaagat aaattccatc aacatcagca agctgcctac ttccgcacgc
     4321 gacatcgcgg aacgcgtgaa cgagatcagc ttcaacagct cgctcatcaa tgaaatgcag
     4381 atggtgcatt accgcaacga gctcatccgg agaggtgttt cacttcccga acagaaccgt
     4441 gaaattttcg ttcactgtat ttccggctac aactcgctcg atcatctttc ctattcgagc
     4501 aagctcaata cctcgtggga atttttgcag cacctgaagg aagaaggaag aaaaaccgcc
     4561 gatgcatggc tcgagaatga atatgatctc gtcggtgtgc gctccacgtt cgatgtggag
     4621 gagcatttcc tcaaacgata ttaagatcaa gcatcacgac gttacatctc agttcgccgt
     4681 tacctgttcg ctgctttttt cattccgttc atttctccgg aaatgaaaaa acgcctgttc
     4741 gatctcgcga acgggaccgt aatagaccgc atccgccatt ttctttccgg gaacgtatag
     4801 ctggaaattt ccgggaggac gaggattcgt ttcgttgctc agtgaactga tgccgtattc
     4861 ttctttcagc gtgagccggc tgttcccgga gctgtcgcag aaattgacgt agcacagtac
     4921 ttttctggat ccgcggtaac cgggcagaat gatgtaatag aactcaatgg aggttatgcc
     4981 ggggaaagag cgttcctccc cgttgcgtga cgttaccgaa gcctcaaaaa cgttctgctt
     5041 catcatgcgc agtgaaaaaa aagagcgcga caggtagtga aatccagcgg cggcaagc
//
LOCUS       DASTSF010000394        14211 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_49746,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000394 DASTSF010000000
VERSION     DASTSF010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 14211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14211
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49746"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..400)
                     /locus_tag="VFU15_11195"
     CDS             complement(<1..400)
                     /locus_tag="VFU15_11195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HEU4718394.1"
                     /translation="MKNCAFFSFVACFLFLPLFTRAQTAAGNTAVMLPNGWRLSPVGK
                     SLPLGDLPLNIAVSPDRKMLAVTNNGVGSQTVQLIDAVSHIQLDAVTVGKSWLGLAFS
                     DDSKNLYVSGGNDNFILRFDIKNKRLLAKDT"
     gene            complement(473..1960)
                     /locus_tag="VFU15_11200"
     CDS             complement(473..1960)
                     /locus_tag="VFU15_11200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718395.1"
                     /translation="MKKSIGTILFLSLVFTSGTFAAQNYAWTRSVVNTGLEFSMQAIC
                     AGEQELCVAGHSAELNYRLGDPGLFDGSGTSLMSEYEISRAGDLLFVYGFDGELRWHY
                     YFDTDEAELEGITYDVNNQLVALVYQHGLDAGDDEEDDGENADENDIYHPAKRRPEDL
                     AGYCLYYFNTTGSIVKNRVLTGLHGIDVTDFKSHPSGGFVIAGHAQNDTVSVHPLLVP
                     GKGGGDLVVFVSAKDSVAWGDMISYDKETCCTFGADPKIAVDEKGNVFLGGSYMHGAR
                     FGGKFMRLAPIPYNPGAYYEAEETYVAAYSPEGKFLWVNTAGTQSWFTGIAAAGGYVY
                     IGTSASRTETLYDHSVDTADHEHVFITVFDAKGKFRRNLSTGAGSKILLSTDAKGNLV
                     ATGTIGIETGYLKRPASDDKTKRRLEDCFVAFYSPGGTFQKCLLTDLVKIPSSDPPVC
                     LAAGPGEIFMIANITGGMSLTLHMLDNAFPNLEFAMGASFVARWK"
     gene            complement(1957..3957)
                     /locus_tag="VFU15_11205"
     CDS             complement(1957..3957)
                     /locus_tag="VFU15_11205"
                     /inference="COORDINATES: protein motif:HMM:NF026253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HEU4718396.1"
                     /translation="MRRTVGLTLFLFFLQLFTLCAQVVPFRKGNEWRLYDKTTQTVLP
                     DTFREIVPASCGLLFINLQGKWGAIDGKKRTVIPARYLELRQLNADVLFARDSRGIHL
                     FDTLGKAVTKTLFKEAQLYRDDSSLIVVRNKKDKYGIIDSHGKTKLKFIYDFAPEHIG
                     GGFLSVGKKAADGSRRVGLLDSLFHAVLPASYTKIALLDNRYFVCSETSGQYALLGYD
                     TKKLYEGIAQPYRVYTNFILTGTSGRSGVYVRKTGKLFLFGLGSYAIWDENGLSLFNE
                     AETNSVFFVTAAGDTAAVHGYRVLTGQDYSPYFPVTPAGDNEQGKWGMIDAKGRLVIP
                     LEYDAINSWNVTCCAAGTYRLNEKGWRVTDYSLIDIASGKPRTEKTYEDIRVLPAGGI
                     ALKENGAYHLYDENLELQDTTEYSSVMTNNVLFYGRPGYPFCAGCALYEVKKRHAKNQ
                     FFTGYVDGHCHVLIPPVFREIKMISDDYNNATPFCRMYGTVYDSTLIYPAYGRAVLLS
                     CDGKQLGGAHVRGISSYSVNNFIPVYNDVRYEDEVRTFAGLMDTLGNVVLPTAYDAVE
                     GIRNTHYFMLRLNGKTGVAGPDGKLILPLVYKSVGAFGTSRLLVLTDAGWGIADAKGN
                     VLLRPAYEKVLAGPLYNGTLFKVSRDKKEFYVDINGTEYTSP"
     gene            4120..5496
                     /locus_tag="VFU15_11210"
     CDS             4120..5496
                     /locus_tag="VFU15_11210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718397.1"
                     /translation="MRTPLTVIFMLLSLLLSAQRCDWMAFTDCGAQMSTTQVCADDRG
                     NVFTGGQYQGEALFHSTAPVDSAIDKYTSTNWRKTFIARYDRRGKLCMVVQFRGDGSN
                     SNSTYLEGMRALPNGNCLAFVMATGRVDFIDPKGNHIETGNNGRGSNLFCIDTLGRLV
                     WSVRAPIEHCRFMETVSGRTIFLFGNSQNTWSNDNLLLKYSAAGVPLDTMYTGGPDLM
                     GMCLKGDRLYAIASNSGFSSRAYSKSRLFEMPGGSMGLYSIDTANLVPSQLFTCPVPM
                     VQDYPKDRQVMFRASVNYTSDVLYADILVTDGGTVKFLGKTYPQSNGNALIIRLDGKG
                     EIVASTTFREHDYGCRITPAPGGAVYILLTAWGNAFHMGSDSLVLPARGEYVYELVMM
                     KLDAHFNRVWWCTAGGTDGIYESYIMATGKHDELLVFAELSAPCNVFGRQATIQWSRT
                     SYVAKLEE"
     gene            complement(5511..6650)
                     /locus_tag="VFU15_11215"
     CDS             complement(5511..6650)
                     /locus_tag="VFU15_11215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006989285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU4718398.1"
                     /translation="MDLFSDDIALEDLETATRLLRESRTGFLKLFNSSPVCMSMTTTN
                     LGKRVYVRVNRMFVEKFGFDESEIIGRTSPEIGILDAEESLRVGAMIKEKGRLHNDYV
                     KCIAKSGKIVHTISSIEMMEMNGETYLVSFFVDITKVMEQQVIIEQHAQELETVNRDL
                     EAFSYSVSHDLRAPLRAINGYAKMLEEDFDSVLDDEGKRILAAVQSNAKKMGNLIDDL
                     LAFAKLGRMQVRKTPVDLDALAGEILSELKKTTGHKAEIRTGKLPPVMGDLSLIRQVL
                     VNLISNAVKYSSIKEKPVVEITAETKDGMVTCTVKDNGAGFDMKYADRLFGVFQRLHS
                     EEEFEGTGVGLAIVQRIVSRHGGTVHAEGEPGKGATFRFSLPAAV"
     gene            complement(7030..12549)
                     /locus_tag="VFU15_11220"
     CDS             complement(7030..12549)
                     /locus_tag="VFU15_11220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718399.1"
                     /translation="MDYKEIYTRLRTCWSDRTLRIPAPTFGEGAMQKVISCFFSDQTL
                     VLASDEGPVKDDAAEKVLVKNATLQSTLLGVSQRRADAEFFADSKGDAHFILRIGANS
                     TPVALENMFPSSAQTALGALRVQDIQIEVASQETHFTGELLRNRGFQQLISIPGDEQF
                     LAPVTTAGARIKAGLPLPAGWEWIAEYLPQGMQQVSGVVEHVGGIHRFVLDTEKGKGY
                     SFGKLNARFNFSLASVAVYVPDDVKEDSFLPVTVFRMGMTVSFVPEGGKEISVPCFTQ
                     WTESLPSEFRFEAHTSEIAEYAVEQFASLLGFDKFPELVPAGANFPPLTAVLLNRITA
                     GVQWRKLQINTLAAEFGLNKDKSFDLIPGLIKVEKLSIVVTVMKPFTSSFSWNADANG
                     IFSIAGTVVSGTMYGAISMPDFSFSFGLTPGSGIDPYSWFEKFMPLPRSFPRFGITAL
                     DVSGSWREKKFDFDSALAFDEGFDLLSVRLRETRLGFSYDGNAGGVAEKSISLSAVLE
                     IGDKAEVFVGGSYYRNGSGSGFELQGEIVFNTPLRVLDFLPEEIKPDPELIAFDVKSV
                     RAKIDTGKPMVEFELNLEKAMVAFPGFGRLSTSRLFIHIEKREGSKEYDWTISISGGL
                     ELLEYEDDDGVKSPLLQLTGDLVLKKDKNKTELYFEKKGDKPVLSNMPLLLPAEFDLE
                     HNKIVWSAMNVDFKKIALAKKTKWEFRVEDVEIHFTRLPHILKDRIPGNKLSFNLFLG
                     YDETTKSTGLRLELNKGLLDWEIPSLRFPKAFGLDGIELGKARLKITKATIVFSNRFG
                     ISVDVQYFLPTNLNVPLEKMGFKEMLQTYDPAQPDKSKHLGITIFGMIEGKSIRLGAK
                     LSEVPISILEKVDEKWWAFHIGDKEEDGMGKFGCIHFEKPELSFDFAKFAFEASGAVK
                     IVKDPAIPLVLIKMLFTEMKRPEIAALLPDKITLPLTKAPPLKDPQTGKYDVNLILKY
                     LGLSPGQFPVVDDIIRRFIEVVNKLPKDFVDYLEPRLPEELAFRFKMTPDGSMEGSFY
                     AKPGFRLLFIGPPGPQILGITLREISFGQMFGGQLLKLRVDAHIDSFDLLSLVLAASV
                     TNTDWYTWMGTPFDFRRRFNLEKMLIFVIWQTQVPIPIPVFFKNFGVEYNGLFNVKAA
                     LGASFPEPEFNLLKLGELLKDLVDFARKPGDSLDENKTLEPFDLKVSLGPAYLQLGKK
                     GSLLNIGSENPLDLPSAYQVLAKGLNTIKHFDLEKAIRSVPEKYRYARLPVTEILPGM
                     KVEGMWMIATPSEFAGGNYALIEINRDRMLELMALLPKREDGSTVTEKDKGVVAFING
                     KWKTEYASVEALFSMVMIKAKHLAVQMRFKGHVSDAIYIFAGGLLKIAPPETPFQLEG
                     KMESRLKLAGLDIYKGSLSIAISANRFNLLGNLGVMQENNLFYLKAENFGGAMGGDYF
                     GLTGKISGKILFLEGGGEATLSLNGIDYWFMVADLKIGLHILKEGDALKIGGDIPIAG
                     INFNSDSWIYPGQLRFRLKLTTADIGGLFSMEFEKETALRSGSIDVDFKLFILKTINN
                     EPVLATSTSIQPHGVRVNGIFKLFHSSSPINLRGDISGEIGLTGFSFGGRVKVVPDLG
                     VLPELIIVITSNSFFLSGKIGPVEFAGKIFAWRDDVVTCITWPGITSPWWIYFSAKHP
                     IPVWEKHPGYDSVFAADGKRARAAKATSPLAALTTLALGFAAEKKFPKAKLSLAMDET
                     HAWAAKRYDWTLKHESQAKGNSRITFTAKRITKGNLKDFFSEVLKSLPSKSPRPSLEN
                     ASLELVFHTMNKKAGIKLTLRFPGAKLERHRKIVVKFTGDEKDDLKKVVKAIAGR"
     gene            complement(12561..14036)
                     /locus_tag="VFU15_11225"
     CDS             complement(12561..14036)
                     /locus_tag="VFU15_11225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718400.1"
                     /translation="MALKIMMWNIEQFGTTRCKYPNRVDRISDIVAHEKPDVFVTLEV
                     RTKDPADAIDVADKIKNGLYNNHNLDYQYILTHYSFFEYYAYFYLDTVVPYTVATGGS
                     VLYDYSNSLQNLTLVRAGNRLSTTNATVNNGTPLLKNHFPLLDYPGRAPQKTNEKNRR
                     PPGAGFFKTTNSNEYFAVITWHNETATQIKRKRGSEKGPMMGKGVVSMQRIAKAGFVQ
                     NSNLIVQVKENGTTSQRTFDQVIFTGDFNNDPTTNKNDFTGLGTFSHMVTNPTHIHNY
                     VDDEGEEFNELIHCLDNSLQSQSLAQNNNIKTKADVFSLNLWLKDKKPGNACLKKMLQ
                     ESWLGDKGALHRLAKEDALRGLDHYCDKRKYGTVYKEYKDEIIRKILNQNLKAWVNKS
                     QTKQTQQTFINDLNNNILANLQVKNRSSEEMTEIRKQLAPVAGEVATIVKDYAKNFCH
                     IVKRYELIKKNPDIIKSSEALFLIRMILSDHLPVITTISNW"
BASE COUNT         3113 a         3731 c         3733 g         3634 t
ORIGIN      
        1 aggtgtcttt cgcgagcagg cgtttgtttt ttatatcgaa gcgcagaatg aaattatcat
       61 tgccgccgga aacgtaaaga tttttggaat catcgctgaa tgccaggccg agccatgatt
      121 ttccaacggt aacagcatcc agctgaatat gggaaacagc atcgatcagc tgcacggtct
      181 ggctgcccac gccgttgttc gttacggcga gcatcttcct gtcgggcgaa accgcaatgt
      241 tcagcggcag gtcgccgagc ggcaggcttt ttcccacggg actcaggcgc cagccgttgg
      301 gcagcatcac cgccgtatta cccgcggctg tttgtgcgcg ggtgaaaagc ggtaaaaaca
      361 aaaagcaggc gacgaagctg aagaaagcgc agtttttcat tggcaaaatg aatataagcc
      421 aaaaatagga tgcgcatgtt acggaaagag atccgggaga ataattccgg cgtcatttcc
      481 agcgcgcgac aaacgaagct cccatggcga actcgaggtt gggaaatgcg ttgtccagca
      541 tgtgaagcgt aagcgacatt ccgccggtga tattggcgat catgaagatc tccccggggc
      601 ctgcggcaag gcaaacgggc ggatcggagc tgggaatttt gacgagatcc gtgagcaggc
      661 atttctggaa tgttccgccg gggctgtaaa atgcgacgaa acaatcttcc agccggcgct
      721 tcgttttgtc atcggaagcg ggccgcttca ggtaacctgt ttcaatgccg atggtgcccg
      781 tggcgacgag gtttcctttc gcatcggtcg acagcaggat cttgctcccc gcaccggtgc
      841 tgaggttgcg cctgaacttt cctttcgcat cgaacacggt gatgaaaacg tgctcatggt
      901 ctgccgtatc gacggaatga tcgtacagcg tttccgtccg cgaagcgctc gtgccgatgt
      961 acacataacc tcctgcagct gctatgccgg tgaaccacga ttgtgttccg gctgtgttca
     1021 cccaaaggaa ttttccttcc ggggaatacg cggccacgta tgtttcttct gcttcgtaat
     1081 acgcgccggg attatacggg atgggcgcga gcctcatgaa ctttccgccg aagcgtgcac
     1141 cgtgcatgta gcttccgccg aggaacacgt tccctttttc gtccacggcg attttcggat
     1201 cggcgccgaa ggtgcagcac gtttctttgt cgtagctgat catgtcgccc cacgccaccg
     1261 aatctttcgc gctgacgaaa accacgagat ctccgcctcc tttgccggga accagcagcg
     1321 ggtgaacgga aaccgtatcg ttctgtgcgt ggcccgcaat gacgaatcct cccgacgggt
     1381 gtgacttgaa atccgtcacg tctattccat gcaggccggt gagcacgcgg tttttcacga
     1441 tgctgccggt ggtgttaaaa taatacaggc aataacctgc caggtcttcg ggccgtcttt
     1501 tcgcaggatg gtaaatgtca ttttcatccg cgttttcccc gtcgtcttct tcgtcgtcac
     1561 cggcgtcgag tccgtgctgg taaacgagcg cgaccagctg gttgttcacg tcatacgtga
     1621 ttccttcgag ctccgcttca tccgtatcga agtaataatg ccagcggagt tctccgtcga
     1681 acccgtaaac gaacaggaga tcgcccgccc gggatatttc atattcgctc atcagcgacg
     1741 tcccgctccc gtcgaagaga ccgggatcgc ccagcctgta gttcagctcg gcggaatgtc
     1801 cggcaacgca cagctcctgc tctccggcgc agatcgcctg catgctgaat tcgagtcccg
     1861 tattcacgac ggaacgcgtc catgcataat tctgcgcggc aaaagtgccg gacgtaaaaa
     1921 ccagcgataa aaaaagaatt gtccctatgc tctttttcat ggcgaagtgt attccgttcc
     1981 gttgatgtcg acgtaaaatt cttttttgtc gcgcgacact ttgaacaatg tgccgttgta
     2041 cagcggtccc gcgagaactt tttcgtaggc gggtcgtagc agcacgtttc cttttgcatc
     2101 ggcaataccc cagcctgcat ccgtgagcac gagcagccgg ctggttccga aagcgcccac
     2161 cgatttgtaa acgagcggga gaataagttt tccgtccggg cccgcaacgc ccgttttccc
     2221 gttgagacgc agcatgaaat aatgtgtgtt gcggatacct tccaccgcgt cgtaagcggt
     2281 aggaagaacc acgttgccga gcgtgtccat cagcccggcg aatgtccgta cttcatcttc
     2341 ataacgtaca tcgttgtaca cgggaataaa gttgttcacg gaatagctgc tgatgcctct
     2401 cacgtgcgca ccgcccagct gttttccgtc gcagctgagc agcacggcgc gcccgtatgc
     2461 ggggtaaatg agcgttgaat cgtaaaccgt tccgtacatg cggcagaacg gggtcgcgtt
     2521 gttgtaatcg tcgctgatca tttttatttc ccggaaaacc ggcgggatca atacatggca
     2581 atgcccgtcg acgtagcccg tgaaaaactg gtttttggca tgcctctttt tcacttcgta
     2641 aagcgcacag cctgcgcaga agggatagcc gggcctgccg taaaaaagaa cgttgttcgt
     2701 catgacggaa gaatactccg tcgtgtcctg tagctcgaga ttttcatcgt acaaatgata
     2761 agcgccgttc tctttcagcg cgatgccccc ggcaggaagc acgcggatat cttcgtacgt
     2821 tttttccgtt ctcggttttc cgctggcgat gtcaatgagc gaataatcgg tcacgcgcca
     2881 tcctttttca ttcaggcggt aagttcccgc cgcgcagcag gtgacgttcc agctgttgat
     2941 cgcatcgtat tccagcggga tcaccagcct gcctttcgca tcgatcatgc cccatttccc
     3001 ctgttcgttg tcgccggccg gagtaaccgg gaagtacggc gaataatcct gcccggtcag
     3061 cacccggtag ccatgcacgg cggcagtgtc gcctgcggcg gtaacgaaga aaacagaatt
     3121 tgtttccgct tcgttgaaca ggctcagccc gttttcgtcc cagatcgcgt agctgcccag
     3181 cccgaagagg aacagttttc ctgttttgcg cacgtatacg cccgacctcc cgcttgtacc
     3241 ggtgagaatg aaattcgtgt aaacacgata aggttgcgct atgccttcgt acagtttttt
     3301 ggtgtcgtaa ccgagcagcg cgtattgtcc gctggtttcg gaacaaacga aataacggtt
     3361 gtcgagcagc gcgatctttg tataagaagc gggcagcaca gcgtgaaaga ggctgtccag
     3421 caatcccacg cgccggctgc cgtcggctgc ttttttaccg acggaaagaa atccgccgcc
     3481 gatatgctcc ggtgcgaaat cgtaaatgaa cttaagcttc gttttcccgt gcgaatcgat
     3541 gatgccgtat ttgtcttttt tgttgcggac cacgatcagc gaagaatcat cgcggtagag
     3601 ctgagcttcc ttaaaaagtg ttttggtgac ggctttgccc agcgtgtcaa acaggtgaat
     3661 gccccggctg tcgcgcgcaa atagaacatc cgcattcagc tgtcgcagct cgaggtatcg
     3721 cgccgggatc acggttctct ttttcccgtc gatcgcgccc cactttccct ggaggttgat
     3781 gaacagcaac ccgcaggaag caggaacaat ttcccggaac gtatcgggca gaacggtttg
     3841 ggttgtctta tcgtacaacc gccattcgtt gcctttacgg aacggcacca cctgcgcgca
     3901 gagcgtgaag agttgcagga agaacaggaa gagggttaat ccgactgtac gtcgcatgaa
     3961 agtaaagcta cgaaaatagg ttcgcgcgtt ttctcccggg cggaagtgtc tttgtggcct
     4021 atttcctttg ccgcggaggc gggaaccggg gatccatatt aaaaatgagc agcggacttt
     4081 cccgcgaaca atgaaaccgt attccgtatt tttgaattca tgcgaacccc gctcactgtt
     4141 attttcatgc tgctttcatt gctgctttcc gcgcagcgct gcgactggat ggcgttcacc
     4201 gattgcggcg cacagatgag cacaacgcag gtctgcgccg atgaccgcgg gaatgtattc
     4261 acgggaggcc agtaccaggg agaagccttg tttcattcta ccgccccggt cgacagcgcg
     4321 atcgacaaat acacatcgac caactggcgc aaaacattta ttgcccgtta tgatcgccgg
     4381 ggaaagctgt gcatggtggt gcagttcagg ggcgacggat cgaattccaa ctcgacttac
     4441 ctcgaaggaa tgcgggcgct tcccaacggg aattgcctcg cgttcgtcat ggccaccggg
     4501 cgcgtggatt tcatcgatcc gaaagggaat catattgaaa cgggaaataa cgggcgcggg
     4561 agcaacctct tctgcatcga tacgctgggc cgcctggtgt ggtcggtgag agcgcccatt
     4621 gaacactgcc gctttatgga aaccgtttcg ggccggacaa ttttcctctt tggcaattcg
     4681 cagaatacct ggtcgaacga caatctcctg ctgaaatatt ctgctgcagg tgttccgctc
     4741 gacacgatgt acacgggcgg acccgacctg atgggcatgt gcctgaaagg cgaccggctg
     4801 tatgcgatcg catcgaacag cggattctcc agccgcgctt attcgaaaag caggctgttc
     4861 gaaatgccgg gaggaagcat gggcctttat tcgatcgata ctgcaaatct tgttccttca
     4921 caactcttca cctgccccgt tccgatggtg caggactatc cgaaagacag gcaggtcatg
     4981 ttccgcgcca gcgtaaatta cacgtcggat gtgctctatg cggatatcct ggtcacggat
     5041 ggcgggacgg tgaaattcct cgggaaaaca tatccgcagt ccaacggaaa tgcattgatc
     5101 attcgccttg acggcaaagg agagatcgtt gcctccacga cgttcaggga gcatgattac
     5161 ggttgccgca ttacacctgc gcccggcggt gccgtgtata tcctgctcac cgcctgggga
     5221 aatgcatttc acatgggttc cgacagtctc gtgctcccgg cgcgcggaga atatgtttac
     5281 gagctggtga tgatgaagct cgatgcgcat ttcaatcgtg tgtggtggtg taccgcgggc
     5341 ggaacggacg gcatttacga atcgtacatc atggcgacgg ggaagcacga tgaattatta
     5401 gtgttcgcgg agctcagcgc accctgcaac gttttcggcc gccaggcgac gatacaatgg
     5461 tcgcgcacat cgtacgttgc gaagctggaa gagtaacatt gtgcgtggat tcagacggcc
     5521 gccggcagtg aaaaacggaa cgtggcgccc tttcccggtt ctccttctgc atgaaccgtt
     5581 cccccgtgcc tgctgacgat gcgctgcaca atagcgagac caacgcctgt tccttcaaac
     5641 tcttcttcgg aatgcaggcg ctggaaaacg ccgaacaggc ggtccgcata tttcatgtcg
     5701 aatcctgcgc cgttgtcttt cactgtgcag gtcaccatgc cgtctttcgt ttccgcggtt
     5761 atttcaacca ccggcttttc ttttatcgaa gaatatttca cggcgttgga aataagattg
     5821 accagcacct gcctgatcag cgaaagatct cccatcaccg ggggcagctt tcccgttctt
     5881 atttccgctt tgtggccggt tgtttttttc agttcggaaa gtatttcacc ggccagggcg
     5941 tcgaggtcga caggtgtttt ccgtacctgc atccggccga gctttgcgaa cgcgaggaga
     6001 tcatcgatca ggttgcccat cttcttcgca ttggactgga cggcggcgag gatacgtttt
     6061 ccttcatcgt cgaggacgga gtcgaaatcc tcctcgagca tttttgcata accgtttatg
     6121 gcgcgcagcg gcgccctcag gtcgtgcgaa acggaatagg aaaaagcttc gaggtcccgg
     6181 ttcaccgttt ccagctcctg cgcatgctgc tcgatgatga cctgctgttc catcactttc
     6241 gtgatatcca cgaaaaagga gacgaggtat gtttcaccgt tcatctccat catctcgatc
     6301 gaggagatcg tgtgcacgat tttcccgctt ttggcgatgc acttcacata gtcgttgtgc
     6361 aaacggcctt tttcttttat catcgcaccg accctcagcg attcttccgc atcgagaata
     6421 cctatttcgg gagaagtgcg cccgatgatc tcggattcat cgaagccgaa tttttccacg
     6481 aacatgcggt tgactctgac gtaaactctt ttcccgaggt tggtcgttgt catgctcatg
     6541 cagaccgggc tgctgttgaa cagctttagg aatcccgtcc tgctttcccg gagcagccgg
     6601 gttgctgttt caaggtcttc gagggcaatg tcgtcagaaa acagatccat gttcggtttt
     6661 tttggttgcc tggttattgc ggtaattcac gatataaaaa gcgcggtgaa gatccctcgc
     6721 aggaattttc gcatgcagga tcaataaaag tatttatgct ttgaaaataa tgaattcaat
     6781 atattatgga ttgtatacaa aaacaaaaaa cggcatgaaa aatcagcagg gataaacgga
     6841 ccgctcccgc cggtcaccgg gcagagaacg gcaaaagtat ttccaactat cctgaaggat
     6901 ttttccgcgg gatttttttg tgaatgaaaa acgggcgacc tgttgcgcgt caacagaaaa
     6961 agaaaggaac cggtaatccc gttaattgtt tttgaattga ttgcatgtct aaaatgcgca
     7021 cgctgttctt cagcgtcccg ctatcgcttt caccaccttt ttaagatcgt ccttttcatc
     7081 ccctgtaaat ttcacgacga ttttcctgtg gcgttccagt tttgcaccag gaaagcgcag
     7141 tgtgagctta atgcccgctt ttttattcat cgtatggaat acgagctcca gcgatgcatt
     7201 ttccagcgaa gggcgcggcg attttgaagg cagcgatttc agcacctcgc tgaagaaatc
     7261 tttcaggttg cctttggtga tgcgtttagc ggtgaaagtg atcctgctgt ttcctttggc
     7321 ctgcgattcg tgcttcagcg tccagtcgta ccgctttgca gcccatgcat gtgtttcgtc
     7381 catcgccagg gatagcttcg ccttcgggaa ctttttttca gctgcaaagc cgagcgccag
     7441 cgtggtgagt gcggccaggg gagaagttgc tttggccgcc cgggcacgtt ttccatcggc
     7501 agcgaaaacg ctgtcataac ccggatgttt ttcccagacg gggatgggat gttttgcgga
     7561 gaagtatatc caccacgggg aggtaattcc cggccatgtg atgcaggtta ccacatcgtc
     7621 cctccaggcg aatatcttgc ctgcaaattc tacgggccca atttttcccg aaaggaagaa
     7681 tgaattcgat gtgataacga taataagttc agggagcacg ccgaggtccg gcacaacttt
     7741 cactctgcct ccgaaactga atcccgtcag ccctatctcg ccgctaatgt ctccccgtaa
     7801 attgatgggg ctgctggaat ggaagagctt gaaaatgcca ttcaccctca ctccgtgtgg
     7861 ctgaatagaa gtgctggtcg ctagtacagg ttcgttgttg atggttttca ggatgaataa
     7921 tttaaaatcc acgtcaatgg aaccggacct gagcgctgtc tctttttcaa actccatgga
     7981 gaatagtccc ccgatgtcgg ccgttgttaa tttcagccgg aaccttaatt gcccgggata
     8041 gatccaggaa tcggagttga agttgatccc tgcgatggga atatcaccgc cgatcttcag
     8101 cgcgtcgcct tccttcagaa tgtgcagtcc gatcttcagg tctgccacca tgaaccagta
     8161 atcgatcccg ttcagtgaaa gcgtggcttc tccgccgcct tcgagaaaca atattttccc
     8221 gcttattttt cccgtgaggc cgaaatagtc tcctcccatg gcaccgccaa aattttcagc
     8281 tttgaggtaa aacaaattat tctcctgcat tacgcccagg ttccccaaca ggttgaacct
     8341 gttcgccgaa atggcgatgc tgaggctgcc tttatagatg tccagcccgg ccagcttcag
     8401 gcgcgattcc attttacctt cgagctggaa cggtgtttcc ggcggtgcta ttttgaggag
     8461 tcccccggca aaaatgtaga tcgcgtccga gacatgacct ttgaaccgca tctgcacggc
     8521 gaggtgtttg gctttgatca tcaccatgga aaaaagagct tccacgctcg cgtattcagt
     8581 tttccatttt ccgttgatga acgccaccac tcctttgtcc ttttcggtga cggtggaccc
     8641 gtcttccctt ttgggcagga gggccatgag ctccagcatc ctgtccctgt tgatttctat
     8701 cagcgcataa tttcctccgg cgaattcgga tggcgtggcg atcatccaca tgccttcgac
     8761 tttcatcccg ggaagaattt ccgtgacggg cagtcttgcg taacggtatt tttcgggcac
     8821 cgagcgtatc gctttctcca ggtcaaagtg cttgatggtg ttgagcccct ttgcaagtac
     8881 ctggtaagcc gagggaagat caagcgggtt ttccgaaccg atgttgagca ggcttccttt
     8941 tttgccaagc tggagatagg ctggtcccag cgaaaccttg aggtcgaagg gctcgagagt
     9001 tttgttttcg tccagcgagt caccgggctt gcgggcgaag tccacgagat ctttcagcag
     9061 ttcgcccagt ttcaacaggt tgaactcggg ttccgggaat gatgcaccga gggctgcctt
     9121 cacgttgaac aggccgttgt attccacgcc gaaatttttg aagaacacag gaatcggaat
     9181 agggacctgt gtttgccaga tgacgaagat gagcatcttc tccaggttga accttctgcg
     9241 gaaatcgaaa ggtgtgccca tccaggtata ccagtcggta ttggtaacag aggcggcaag
     9301 cacgaggctc agcaggtcga aggaatcgat atgtgcgtcc accctgagct tgagcagctg
     9361 cccgccgaac atttgtccga atgaaatttc gcgcagtgtg atgccgagta tctgcgggcc
     9421 gggagggccg atgaacaaca gcctgaatcc gggctttgca tagaacgatc cttccatcga
     9481 tccgtcgggc gtcatcttga aacggaaagc cagttcttcc gggagccgtg gctcgaggta
     9541 atcgacgaaa tctttcggca gcttgttgac cacttcaatg aatctgcgga taatgtcatc
     9601 tactaccggg aactgaccgg ggctgagccc gagatatttc aggatcaggt ttacgtcgta
     9661 tttgccggtt tgcggatcct tgagcggcgg cgctttggtc agtggaagcg tgatcttgtc
     9721 aggaagcagg gcagcgatct ccggccgttt catttccgtg aacagcatct tgatcagcac
     9781 gagcgggatg gcggggtctt tgacaatttt taccgctccc gatgcttcga aagcgaactt
     9841 cgcgaaatcg aatgacagct ccggtttttc aaaatgaatg cagccgaatt ttcccattcc
     9901 gtcctcctct ttgtccccga tatggaacgc ccaccatttc tcatccactt tttccaggat
     9961 ggagatggga acttcggaaa gttttgctcc gagccggatg gatttgccct cgatcattcc
    10021 gaagatcgtg atgccgagat gcttgctttt atccggttgt gcgggatcat aggtttgcag
    10081 catttccttg aatcccattt tctccagggg aacgttcagg ttggtgggca ggaagtactg
    10141 cacatccacc gaaatgccga aacgattgct gaaaacgatc gtggctttgg tgatcttcag
    10201 gcgcgccttg cccagctcga tgccgtcgag tccgaatgcc ttcgggaatc tgagcgaggg
    10261 aatttcccaa tcgagcaaac ccttgttcag ctcgagacga aggcccgtgc ttttggttgt
    10321 ttcgtcatag ccgaggaata aattgaacga aagcttgttg ccgggaatgc ggtctttgag
    10381 gatgtgagga aggcgggtaa aatggatctc gacgtcttcg acgcggaact cccatttcgt
    10441 ttttttcgcg agcgctattt ttttgaagtc tacattcatt gccgaccaca caattttgtt
    10501 gtgctccagg tcgaattcgg cgggaagcag cagcggcata ttgctgagca cgggcttgtc
    10561 tcccttcttc tcaaaataca gttcggtttt gttcttgtct tttttcagca caaggtcacc
    10621 ggtgagctgc aacagcggac tcttcacgcc atcgtcatct tcatattcga gcagctccag
    10681 tccgccggag atggaaattg tccagtcgta ttctttgctg ccttcgcgct tttcgatgtg
    10741 gatgaacagg cggctggtgc tgagcctgcc gaacccggga aaagcgacca tcgctttttc
    10801 gaggttcagc tcgaattcaa ccatcggttt tcccgtgtct atttttgccc gcacgctctt
    10861 gacgtcaaaa gcgatgagct cgggatcggg tttgatttct tccggcagaa aatccagcac
    10921 cctgagcggc gtattgaata cgatttcccc ctgcagctca aaaccggatc ccgatccgtt
    10981 cctgtaataa cttcccccga caaacacttc cgctttgtcg cctatttcaa ggacggctga
    11041 cagcgagatg gatttttcgg caacgccgcc cgcgttcccg tcatagctga agccgaggcg
    11101 ggtttcccgc aacctcaccg aaaggagatc gaatccttcg tcaaacgcaa gtgccgagtc
    11161 aaaatcgaat ttcttttctc tccatgatcc cgaaacatcc agcgccgtga tcccgaaccg
    11221 cgggaacgac cggggcaatg gcataaattt ttcaaaccag gaatacggat ctatgcccga
    11281 gccgggtgtg agcccgaagc tgaaagagaa atcgggcatc gatatggctc cgtacatagt
    11341 tccgctcacc actgtgccgg caatgctgaa gatgccattg gcgtctgcgt tccaggaaaa
    11401 agaagaagtg aaaggcttca ttaccgtaac cacaatggaa agtttctcca ctttgatcag
    11461 tcccgggatc aggtcgaacg atttgtcttt gttcagcccg aattccgcag cgagcgtatt
    11521 gatctgcaat tttctccatt gtactcctgc tgttatcctg ttcagcagca ccgcggtgag
    11581 cgggggaaaa ttggcaccgg cgggcacaag ctccggaaat ttgtcgaagc ccagcaacga
    11641 agcaaactgc tccacggcat actctgcaat ttcactggta tgcgcctcga agcggaactc
    11701 cgagggaagg ctttccgtcc attgggtgaa gcagggcacg ctgatctcct ttccaccttc
    11761 gggaacgaaa gagacggtca ttcccattct gaaaaccgta accgggagga atgaatcttc
    11821 cttcacgtca tcaggcacgt ataccgccac cgacgccagc gaaaaattaa aacgggcgtt
    11881 aagcttgccg aacgaatatc cttttccttt ttccgtgtcg agcacaaaac ggtgaatgcc
    11941 gccgacatgc tccaccacgc cgctcacctg ctgcattccc tgcggaagat attccgcgat
    12001 ccattcccat ccggcgggca ggggaagccc ggctttaatg cgcgcacctg ctgtagtgac
    12061 cggcgcaaga aactgctcat caccgggaat gctgatcagc tgctggaacc cgcgattgcg
    12121 caacagctct ccggtgaaat gtgtttcctg cgaagcaact tcgatctgta tatcctggac
    12181 ccgcaaggca ccgagcgctg tctgtgcaga agaagggaac atgttttcaa gcgccaccgg
    12241 cgtgctgttt gccccgatcc gcagaatgaa atgcgcatca cctttgctgt cggcaaaaaa
    12301 ttccgcatcc gccctgcgtt gcgaaacacc cagcagcgtg gattgcagtg tggcgttttt
    12361 tacaagcact ttttcagccg catcatcttt taccggacct tcatcggaag ccagcaccag
    12421 cgtttgatcg gaaaaaaaac agctgatcac tttttgcatc gctccttcgc cgaaagtagg
    12481 agcaggaatg cgcagcgtcc ggtcagacca gcaggtccgg agcctggtgt aaatttcttt
    12541 gtagtccatt gcagaaaagc ttaccagttg gaaatcgtag tgatgacggg aaggtgatcg
    12601 ctcaggatca tccggatgag aaagagggct tcagaggatt tgatgatatc gggattcttt
    12661 tttatcagtt cataacgctt aacaatatgg cagaaattct ttgcatagtc tttcacgatc
    12721 gtggctacct ctcccgccac aggcgccagc tgcttcctga tctcggtcat ttcctcactt
    12781 gatctgttct tcacctggag gttggccaat atgttattat taaggtcgtt gatgaaggtc
    12841 tgctgcgtct gtttcgtctg ggatttattc acccaggcct tcaaattctg attcaggatt
    12901 ttcctgatga tctcatcctt gtattcctta tagacggtgc cgtattttct cttatcacaa
    12961 taatggtcca ggcccctgag cgcgtcttcc ttcgccaggc gatggagcgc gcccttgtcc
    13021 cccagccagc tctcctgcag catttttttc aggcaggcat tgcccggctt tttatctttc
    13081 agccagaggt tgagagaaaa aacatccgct ttcgttttta tattgttgtt ctgcgcaaga
    13141 ctttgcgatt gcagcgaatt atccaggcaa tggatcagtt cgttgaattc ttcaccctcg
    13201 tcgtcaacat aattgtgaat atgcgtcggg ttggtgacca tgtgtgaaaa tgttccgagc
    13261 cctgtgaaat cgtttttatt cgtggtggga tcgttgttga aatcgccggt gaagatgacc
    13321 tggtcaaaag ttcgttgact ggtcgtcccg ttttctttga cctggacaat gaggttgctg
    13381 ttctgaacga atcccgcttt ggcgatgcgc tgcatgctca ccactccctt gcccatcatc
    13441 gggccttttt ctgaaccgcg ctttcttttg atctgggtcg ccgtttcgtt gtgccaggtg
    13501 atcacggcga aatattcatt cgagttcgtc gtcttgaaga aacctgcgcc cggcggcctc
    13561 ctgttttttt catttgtttt ttgcggcgcc cttcccgggt aatcgaggag cgggaaatga
    13621 tttttgagca ggggtgttcc gttgtttaca gtggcattcg tcgtgctcag ccggttaccg
    13681 gctcgtacca gcgtaaggtt ctgaagggag ttggaataat catagaggac cgatccgccc
    13741 gtggcgacgg tatacggtac aactgtatcc aggtagaagt aggcgtagta ttcaaaaaaa
    13801 ctgtaatgcg tgaggatgta ttgataatca agattatgat tattgtacag cccattttta
    13861 attttgtccg caacatcgat cgcgtctgcc ggatctttgg tgcgcacttc cagggtgaca
    13921 aagacgtcgg gcttttcatg cgccacaatg tctgaaattc ggtccacgcg attcgggtat
    13981 ttgcagcgcg tagttccaaa ctgctcgata ttccacatca tgatcttgag ggccataatc
    14041 cgacaatttt tcgtttaatt ttcagaaatg taattggttc ccggaagata gtcgtttaca
    14101 gtttcgaaaa cgaactgtca gcaggtaaag tacattttta taacctgaat ttgattttac
    14161 agcgctccat aaaaagtgga gcgtgccatt gctgttgccg cctttttttc a
//
LOCUS       DASTSF010000395         9503 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1368773,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000395 DASTSF010000000
VERSION     DASTSF010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 9503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9503
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1368773"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..309)
                     /locus_tag="VFU15_11230"
     CDS             complement(<1..309)
                     /locus_tag="VFU15_11230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718401.1"
                     /translation="MRIILYFLVFSCVSLRVYSQEQSMLYEKDWGVNDGCGLYYADEL
                     PNGDYILIGGRRSALYPPGTGYFQNYMCRINFRGDLLWEKEIGFPYAQDYGGGVIKTS
                     S"
     gene            471..2954
                     /gene="hrpB"
                     /locus_tag="VFU15_11235"
     CDS             471..2954
                     /gene="hrpB"
                     /locus_tag="VFU15_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013159220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HEU4718402.1"
                     /translation="MSLSLPIEEVLEEVKSKLASHNTVILQAPPGAGKSTWLPVQLLN
                     EPWLNGKKIVMLEPRRLAARTVAARLAFQVEEETGETVGYRIRFEKKVSARTRVEILT
                     EGILTRMLHEDGALEEAGLVIFDEFHERSLHADLALAFCLEVQKILRPDLRILIMSAT
                     LDGEKLSRLLGDAPVITSAGRQHPVSFHYTGYDDSLPLPLEISKIIRRALQEEEGDIL
                     AFLPGTGEIMRCAEILGNENVPAKVVPLYGDLSMQKQQEAIQPDPYGARKVVLATSLA
                     ETSLTIEGIRVVVDSGFSRVPRFDPSSGLTKLTTVRVTRDSAEQRAGRAGRLGPGVCY
                     RMWTEGFHHHLVPHRSPEILEADLAPLTLDLAGWGTKDIASLTWLSPPPAGAIAQAKE
                     LLEELGATAAGKITERGKEMLRLPAHPRIAHMLLEGKKENLAALATDLAALLEERDPF
                     TRESSADIVLRIEALRKFRSGAPVPADKKRIERIERSAAVWRNYFRIGPENNFPAATD
                     AGKLIALAYPERIAKKEHELRYRLSGGRMLRLKEHDPLASEEYIAVASLDAGTKGEGK
                     IFLAAALDPDDLLHLAYEKKTIAWDAQKGELIARTEKRIGGLILETKPLKDISAEEKT
                     GVLIAAIREGGTGMLNFNEEFLSLQARTLSLRKWREEETWPDLSTEHLFATLEEWLAP
                     YLENVKKKDDLFRLNLHDILLASVSYDQQQRMEKLLPQKIGVPSGSLIKIEYHADGSP
                     PVLAVRLQELFGMSETPKINEGRTKLMIHLLSPAYRPVQVTQDLASFWKNTYAEVRKE
                     LRMRYPKHSWPEDPLTAEAVRGAKRKNRS"
     gene            complement(2977..4341)
                     /locus_tag="VFU15_11240"
     CDS             complement(2977..4341)
                     /locus_tag="VFU15_11240"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEU4718403.1"
                     /translation="MQIRTRLTLQFTGLVAVIFLISYFIIYYSQAQFRISQFNDRLRD
                     KARTTAELLISVDAVTPELLKIIDRNRKDVLYKENISVYNRLNEEIYTSNDTTDFNVT
                     PVMMASVRAEGEQHWRQGDFELIGIPYSDKHNSFVVVAGAIDVYGINKLRNLRNTLLI
                     SSLLFLSIVAVAGWFYAGRSLRPINRIIDQVKTISGTNLVTRLDEGNKRDEIARLSAT
                     FNEMLGRIETTFQLQKAFVANASHELINPLTIITSQVEVTLLRERTPEEYRNTLLSVL
                     EDMRTLNQVSLRLLDLARLNSEELKVNFSHVRIDELIWQCKDEFSEKRPQCKVFFSPE
                     LPEEERQLLVMANEYLLKTAIINLMDNACKFSSDKEVFVTMKASGTEVKVQFRDNGIG
                     ISESDLPFIFQPFYRSHNAVSIRGHGIGLSLVEKILKLHKATVSVESTPGKGSVFTIV
                     IPTT"
     gene            complement(4346..5026)
                     /locus_tag="VFU15_11245"
     CDS             complement(4346..5026)
                     /locus_tag="VFU15_11245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012929274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU4718404.1"
                     /translation="MQRILIIEDEVKTAQSIRQGLEEQQYKVDIAYDGEMGKRLATRH
                     KFDLIISDIILPGINGIELCRQLRAEKIQTPVLMLTALSTVDDKVEGFESGADDYLPK
                     PFEFKELLARIRAMLKRNPQTNTADREITVADLSLNLDNRKATRGGKEIELTAREFAL
                     LEFFMRNVGRVLSKVEIAERVWGIDFDTGTNVVEVYINYLRKKIDREFPLKLIHTQFG
                     MGYIMKEK"
     gene            complement(5354..7987)
                     /locus_tag="VFU15_11250"
     CDS             complement(5354..7987)
                     /locus_tag="VFU15_11250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU4718405.1"
                     /translation="MKNYKFKELKTFSSDEWMLNSSKKYRKVFDKAETTYIRAEFSFY
                     NKLFDEEDWSCTILMKCFSLDETGNKKELCSLDTKRDVKMDENIVYLRDGWGNATEGA
                     YWKEGNYQWEAYIDNELVGTAKFIVNDVGKVTSASNPYFTVKEMKLFVGDYNAWEKKE
                     RTYLKKIDRSKTQYVWAECIIESKTNLAWNYELFFNFLDDAYQPKGQVLREGKVEKGK
                     AGFTYTYEVGWGNDTPGSWKDDKYALEMIFMDTMVGMIEFDCGTEEEQGVPVLVTSSQ
                     HMVPVQAEGTQGGNAQQQPVVEETMDQLLEKMDALTGLTEIKKNIRDHISYLNFIKLR
                     KEKGFEESGKISLHSVFTGNPGTGKTTVVNMLGKIYRKMGLLSKGHVQEVDRADLVGE
                     YIGQTAPKTKKAIEQARGGILFIDEAYSLSRAGEDSRDFGKEVIEILLKEMSDGPGDI
                     AIMVAGYPDEMDTFIDSNPGLKSRFSHYYHFDDYTPEELTAIAAGAAAKKGVKLSGDA
                     EKFLAEQLVELYRTRDKTFGNARLANGIVDEAKMNMGLRLMKVPDLSTLSGDALSTIE
                     IDDIRNAFGSKQKKRADLGINEKLLRSAMDELNSLIGMENIKTEVNELIKLVRFYREI
                     GKDVLNRFSLHAVFTGNPGTGKTTLARIISTIYKGLGLLEKGHIVEVDRQALVASYVG
                     QTANKTQARVEEAIGGVLFIDEAYALGEGGENDFGKEAVEVVLKRMEDMRGQFAVIVA
                     GYPDNMHRFLETNPGLKSRFDRYYVFNDYTSDELFLIAKALLEKEGLAAVPEAEDHLK
                     KYLAFLYDNRDPYFGNARTVRNVVGEAVKNQNLRMASMPSTERTPQILGQLIMADVEE
                     FRLEEGKGRRVQLGFRYGQGN"
     gene            complement(8045..8425)
                     /locus_tag="VFU15_11255"
     CDS             complement(8045..8425)
                     /locus_tag="VFU15_11255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718406.1"
                     /translation="MKFLKREKHRLTTLQRQIMKWLKMEGGGCLPADRLFTTMVKNHY
                     SPDANEELLSQRMCEAVENLVRMEYAEVRLENRTKDRKLPSLFDFAPLGGHMQSDGEK
                     FSWIKGECPVIALSDAGSRYADTL"
     gene            complement(8439..>9503)
                     /locus_tag="VFU15_11260"
     CDS             complement(8439..>9503)
                     /locus_tag="VFU15_11260"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEU4718407.1"
                     /translation="YMTALPEWVNTFNARKLPQSYLSKDWETLLPIDQYTESIADDNK
                     YEATYAGEDKPVFPHHLPLLMDKNGKLGMIRATPFGNSLTREFAQATILGENLGKGSA
                     TDFLCVSFSSTDYIGHQFGPNSVEVEDCYLRLDKELAEFFSFLDNYLGKNNTLLFLTA
                     DHGAVENPQYLKDNNIPAGYFSEGKMTDSLKKVMRKIYGDSLVLCYENDQVFLDRAGI
                     AKKGLNKSEIEKFTASFIMGFKGVEMCMTASDLQSNEYTQKPQQLIRNGFNFQRSGDV
                     CVVLQPSWFSEFGKKGTTHGTSWSYDTHVPMYWWGWKIKNGSSDALMNITDVAPTVCN
                     FLNIQFPDGCTGQPITGMVK"
BASE COUNT         2072 a         2667 c         2461 g         2303 t
ORIGIN      
        1 cgaactcgtt ttaataaccc ctccaccgta atcctgcgcg tacggaaaac caatttcctt
       61 ttcccaaaga agatctcccc ggaaattaat gcggcacatg taattctgga agtaaccggt
      121 tcccggagga taaagtgctg accttcttcc tccaattaat atataatctc cgtttggaag
      181 ctcgtctgca taatacaagc cacaaccgtc attgactccc cagtcttttt cataaagcat
      241 gctttgctcc tgggaataaa ccctcaggga tacacaacta aaaacaagaa aataaagaat
      301 gatccgcatt tcaaagattc ttaaaacccg gccaggattt aaaaccaggc agggctttat
      361 tacaatataa cgtttatttt tttcacactt gagttttttt tcatcagacc aaagacccgc
      421 tgattttagg aacccgcccg gcaaatgcca tcctctatct ttgcggcgag atgtcgctgt
      481 ccttacccat cgaagaggtt cttgaagaag taaagtcaaa gctggcttcg cacaacaccg
      541 tcatcctgca ggccccgccg ggcgcaggaa aaagcacgtg gctgcctgtg cagctgctga
      601 acgagccgtg gctgaacggg aaaaaaattg tgatgctgga accgagaagg cttgcggcgc
      661 ggaccgtggc ggcgcggctt gccttccagg tggaagaaga aaccggggaa accgtcggtt
      721 accgcatccg tttcgaaaaa aaagtttcag cacggacacg cgttgaaatt ctcaccgaag
      781 gaattctcac gcgcatgctg cacgaagacg gcgcgctgga agaagcgggg ctcgtcatct
      841 tcgatgaatt ccacgagcga agccttcacg cggatcttgc actcgcgttt tgtctcgaag
      901 tacagaaaat cctccggccc gacctgcgca ttctcatcat gtcggcaaca ctcgacgggg
      961 aaaaactttc ccgcctgctc ggcgatgcac ccgtgatcac aagtgcgggg aggcagcacc
     1021 cggtgtcttt tcattatacc gggtacgacg attcgctgcc gcttccgctg gaaatttcga
     1081 aaatcatcag gcgcgcactg caggaagaag aaggcgacat cctcgccttc cttcccggca
     1141 ccggcgagat catgcgctgc gcggaaattc tcggaaatga aaatgtgccc gcgaaagtcg
     1201 ttccgcttta cggcgatctt tccatgcaga aacagcagga agcgatacag cccgatccgt
     1261 acggcgcgcg gaaagtcgtt cttgcaacaa gcctcgccga aacttcgctc acgatcgaag
     1321 gcatacgcgt cgtcgtcgac agcgggtttt cccgcgtgcc gcgtttcgat ccttcgagcg
     1381 gactgacgaa gctcacgacg gtgcgcgtca cccgcgattc cgccgagcag cgcgccggtc
     1441 gtgcgggaag gctcggtccc ggcgtttgct accgcatgtg gacggaagga ttccatcacc
     1501 atctcgtccc gcaccgttca ccggaaatcc tcgaagcgga ccttgcgccg ctcacgctcg
     1561 atcttgcagg atggggaaca aaagacatcg catcgctcac ctggctctcg cctcctcctg
     1621 ctggcgcgat cgcgcaggcg aaggaattgc ttgaagagct cggtgcgaca gcagcaggaa
     1681 agatcacgga gcgcggaaaa gaaatgctgc gtcttcccgc gcacccgcgc attgcgcaca
     1741 tgctgctcga aggaaaaaaa gaaaatctcg ccgcgctggc gacagatctt gccgccctgc
     1801 tcgaagagcg cgatccgttc acacgggaaa gcagcgcgga cattgtcctc cgcatcgaag
     1861 cgctgcgtaa attccgcagc ggcgctcctg ttccggcgga taaaaaaagg atcgagcgca
     1921 tcgagcgttc ggctgccgtg tggcggaatt atttccgcat cgggccggag aataattttc
     1981 ctgctgcgac ggacgcagga aagcttatcg cgctcgccta cccggaacgc atcgcgaaaa
     2041 aagaacatga gctgcgctac cgcctttccg gcggacggat gctgcggctg aaagagcatg
     2101 atccgctcgc atcggaagaa tacatagcgg tggcttcgct cgacgccgga acaaaaggcg
     2161 aaggaaaaat ttttctcgcg gcggcgctcg acccggatga tcttcttcat ctcgcttacg
     2221 aaaaaaagac gatcgcctgg gacgcgcaga aaggagagct gatcgcacga accgaaaaaa
     2281 ggatcggcgg actgatcctc gagaccaaac cgctgaaaga catcagcgct gaagaaaaaa
     2341 cgggtgtgct gatcgccgcc atccgtgaag gcgggaccgg catgctcaac ttcaacgaag
     2401 aattcctttc gctccaggcc cggacccttt cgctgagaaa atggcgggaa gaagaaacct
     2461 ggccggacct ttcgacggaa catcttttcg ccacgctcga agaatggctg gctccttacc
     2521 tcgaaaacgt aaagaaaaaa gacgacctgt tccggctgaa cctgcacgac atcctgctcg
     2581 cgtcagtcag ctacgatcag cagcagcgca tggagaaact ccttccgcaa aagatcggag
     2641 tgcccagcgg ttcgctgatc aaaatagaat atcacgccga tggaagtccg cccgtccttg
     2701 ccgtccggct gcaggaattg ttcgggatgt cggaaacgcc gaagatcaac gaaggcagaa
     2761 caaagcttat gatccacctg ctttcacccg cgtaccgtcc cgtgcaggta acgcaggacc
     2821 tcgcgagctt ctggaaaaac acgtatgccg aagtgcggaa agaattgcgc atgcgttacc
     2881 cgaaacattc ctggccggaa gatccgctga cagcagaagc agtgcgcggc gcaaaaagaa
     2941 aaaacaggag ctgaccggga cgttttttcc gtgcgcttac gttgtaggaa tgacgatcgt
     3001 gaacacggag cctttccccg gcgtgctttc cacgctgacc gtcgctttgt gcagcttcag
     3061 gatcttctcg acgagcgaaa gcccgatgcc gtgcccgcga atggaaaccg cgttgtggct
     3121 ccggtaaaaa ggctgaaaaa taaacggcag gtcgctttcg ctgatgccga tgccgttgtc
     3181 cctgaactga actttcactt ccgtgccgga cgctttcatc gtaacaaaaa cttccttgtc
     3241 ggacgaaaat ttgcaggcgt tgtccatcag gttgatgatc gctgttttca gcaggtattc
     3301 attcgccatg acaaggagct gcctctcctc ttcgggaagc tccggggaaa aaaacacttt
     3361 gcactgcggg cgtttctcgg agaattcatc cttgcattgc cagataagct catcgatgcg
     3421 cacatgcgaa aaattcactt tcagctcttc cgaattgagt cgcgcgagat cgagcaggcg
     3481 gagtgaaacc tggttgagcg tgcgcatgtc ttccagcacg gaaaggagcg tgttgcggta
     3541 ttcttccggc gtccgttcgc ggagcagcgt cacttccacc tgcgacgtga tgatcgtaag
     3601 cgggttgata agctcgtgcg aagcgtttgc cacgaaggct ttctggagct ggaacgtcgt
     3661 ttcgatgcgc ccgagcattt cgttgaaagt ggctgaaaga cgcgcgatct cgtctctctt
     3721 gttcccttca tcgaggcgtg tgacgaggtt cgttcccgag atcgttttca cctggtcgat
     3781 gatacggttg atcgggcgca gcgagcgccc tgcatagaac cagcctgcaa ccgccacaat
     3841 ggaaaggaag agcagcgagg agatgagcag cgtattccgc aggttgcgca gcttgttgat
     3901 cccgtaaaca tctattgcac cggcaacgac aacgaagctg ttgtgtttgt cgctgtacgg
     3961 aataccgatc agttcgaaat cgccctgcct ccagtgctgc tcgccttccg cgcggacact
     4021 tgccatcata accggcgtca cattgaaatc cgtagtgtcg ttgctggtgt aaatttcttc
     4081 gttcagccgg ttatagacgg atatattttc cttgtacaaa acgtctttcc ggttccggtc
     4141 gatgatcttc agcagctcgg gcgttaccgc atccacgctg atgagcagct cggcggtcgt
     4201 tctcgcttta tcgcggagac ggtcgttgaa ctggctgatg cggaactgcg cctgcgaata
     4261 gtaaataatg aagtaggaga tcaggaaaat gacggctacc aggccagtga actgcaaagt
     4321 aagccgtgtg cggatctgca tcttgctatt tttctttcat gatgtagccc atcccgaatt
     4381 gcgtatggat aagcttcagc ggaaattccc tgtcgatttt ctttctaaga taattgatat
     4441 acacttccac cacgttggtg ccggtatcga aatcgatgcc ccacacccgt tccgcgattt
     4501 ccactttgga cagcacacgt cccacattgc gcatgaaaaa ttccagcaat gcgaattccc
     4561 gcgccgtcag ctcgatctct tttcctccgc gcgtcgcttt gcggttatcg aggttcagtg
     4621 aaagatccgc aacggtgatt tcacggtccg cggtattcgt ctgcgggttg cgcttgagca
     4681 tggcgcggat gcgcgcaaga agttccttga actcgaacgg tttcgggagg taatcgtccg
     4741 cgcccgactc gaagccttcc actttatcgt caacggtgct gagcgcggtc agcatcagga
     4801 cgggcgtctg tattttttca gcgcgcagct ggcggcaaag ctcgatgcca ttgatgccag
     4861 gcaggatgat gtcgctgatg ataagatcga acttgtgccg cgtggcaagc cgttttccca
     4921 tttccccgtc gtacgcgata tccactttgt actgctgttc ttcgagtccc tgcctgatcg
     4981 actgggccgt cttcacttca tcttcgataa tgagaattct ttgcatggcg ggtaaaaatg
     5041 attctttttt atgaatactg ccttcaaatt tccgtggaag cttcagttca gcaggtaaga
     5101 aaaatcaatt tcataaaaaa acaggagacg aaaacaggaa tttcttccca cctggtgttt
     5161 cgccttcctg ccgggagatt gatgcaactt tttcctgatc cgaaacgatt ccatctcaaa
     5221 aatagaggtg atgccgctcc tgtcaattag aaactgatta aaaggaaagt ggcgccgccg
     5281 gtgctttctc cccgcggttt tgtcttccgg aaaacagaac ccggatcgcc cgaaacatcc
     5341 gcggacctgc cgctcagttt ccctgcccgt aacggaagcc cagctgaaca cgccggcctt
     5401 ttccttcctc gagcctgaac tcttccacgt ccgccattat cagctggccc aatatctgcg
     5461 gtgtgcgttc cgtcgaaggc atggatgcca tgcggagatt ctggttcttc accgcttccc
     5521 cgacgacatt ccgcacagtg cgcgcattgc cgaaatacgg atcgcggttg tcgtaaagga
     5581 acgcaaggta ttttttcagg tgatcttccg cttccgggac ggctgccagg ccttctttct
     5641 ccagcagcgc tttcgcaatc aggaacagct cgtccgacgt gtaatcattg aaaacataat
     5701 agcggtcgaa acgcgatttg agaccgggat tggtttcgag gaaccggtgc atgttgtcgg
     5761 gatagccggc cacgatcacg gcgaactgcc cgcgcatgtc ttccattctt ttcagcacga
     5821 cttccaccgc ttccttcccg aaatcatttt caccgccttc gccgagcgca tacgcttcgt
     5881 caatgaaaag tacgccgccg atggcttcct ccacgcgcgc ctgcgttttg ttcgccgtct
     5941 gcccgacgta cgatgcgacg agcgcctggc gatcgacttc cacgatgtgt cctttctcca
     6001 gcaggccgag tcctttgtag atcgtggaga tgatgcgcgc gagcgtcgtc ttgcccgttc
     6061 ccgggttgcc cgtgaacacg gcgtgcagcg agaaacggtt gaggacatcc ttgccgatct
     6121 cgcggtaaaa acgcacgagc ttgatgagct cgttcacttc ggtcttgatg ttctccatgc
     6181 cgatgagcga gttcagctcg tccatggcgc tgcgcaacag tttttcattg atgccgaggt
     6241 cggctctttt cttctgcttg ctgccgaatg cgttccggat gtcgtcgatc tcgatggtgg
     6301 acagcgcgtc cccggaaagc gtggaaagat ccggcacttt catgagacga agccccatgt
     6361 tcattttcgc ttcatccacg atgccgttcg caaggcgtgc attcccgaat gttttgtcgc
     6421 gcgtgcggta caattccacc aactgctcgg cgagaaattt ctcggcgtcg ccggaaagct
     6481 tcacgccttt tttcgcagct gcaccggccg cgatcgcggt gagctcttcg ggcgtgtaat
     6541 cgtcgaaatg atagtaatgc gaaaacctcg atttcagccc ggggttcgaa tcgatgaacg
     6601 tatccatttc atcgggatag ccggccacca tgatcgcgat gtcgccggga ccgtcggaca
     6661 tttccttgag cagtatctcg atgacctctt ttccgaagtc gcgtgaatcc tcacccgcac
     6721 gtgaaagcga atacgcctcg tcgatgaaaa gtatcccgcc cctcgcctgc tcgatcgctt
     6781 tcttcgtctt cggcgccgtc tgcccgatgt attcacccac aagatccgcg cggtcgacct
     6841 cctgcacatg gcccttgctg agcaatccca ttttcctgta aatcttcccg agcatgttca
     6901 ccaccgtcgt tttccccgtg ccgggattac cggtgaacac gctgtgcagc gagatcttcc
     6961 cgctttcctc gaatcccttt tctttccgca gcttgatgaa gttgaggtag ctgatgtggt
     7021 cgcggatgtt cttcttgatc tcggtgagcc cggtgagcgc gtccatcttt tcgaggagct
     7081 ggtccatcgt ttcctccacc accggctgct gctgcgcatt tccgccctgc gttccttccg
     7141 cctgcacggg aaccatgtgc tgcgatgaag tgacaagcac gggaacgccc tgctcttctt
     7201 ccgtcccgca gtcgaattcg atcatgccca ccatcgtgtc catgaagatc atctcgagcg
     7261 cgtatttgtc gtctttccag gagcccggcg tatcgttgcc ccagcctact tcataggtat
     7321 acgtgaatcc cgcttttcct ttttcaacct ttccttcccg cagcacctgt cctttcggct
     7381 ggtacgcgtc atcgagaaaa ttgaagaaca attcgtagtt ccaggccagg ttcgttttgc
     7441 tttcgataat gcattccgcc cagacgtact gtgttttcga gcggtcgatc tttttcaggt
     7501 acgtgcgttc cttcttttcc catgcgttgt agtcgccgac aaaaagtttc atctccttca
     7561 cggtgaaata aggattcgag gcggaggtca ctttgcccac atcgttgacg atgaatttcg
     7621 cggtgccgac cagctcgttg tcgatgtacg cttcccattg gtaatttcct tctttccagt
     7681 acgcgccttc cgtcgcattt ccccagccgt cgcgcaggta aacgatattc tcgtccatct
     7741 tcacatcgcg tttcgtatcg agcgagcaca gctctttttt attcccggtc tcgtcgagtg
     7801 aaaaacattt catcaggatc gtgcacgacc agtcttcttc atcgaaaagt ttattgtaaa
     7861 acgaaaattc cgcacgtatg taagtcgttt cggctttatc gaatactttc ctgtatttct
     7921 tggaggaatt gagcatccat tcgtccgatg agaacgtttt cagctccttg aatttgtagt
     7981 ttttcacggg taatgattta ggcgtgatga atttaagaaa aggctttcat ccgcaaaaca
     8041 gaggtcagag cgtgtctgca taccgcgaac ctgcatcgct gagcgcgatg acgggacatt
     8101 cgccttttat ccaggaaaat ttctccccgt cgctctgcat gtgtccgcca agcggcgcga
     8161 aatcaaaaag tgaaggcagc ttccggtcct tcgtgcggtt ttcgagacgc acttccgcgt
     8221 actccatgcg cacgaggttt tccacggcct cgcacatgcg ctggctgagc agctcttcgt
     8281 tcgcgtcggg ggaataatga ttcttcacca ttgtcgtgaa aagacggtcg gccggcaggc
     8341 atcctccgcc ctccatcttc agccacttca tgatctgccg ctgcagtgtc gtcagccggt
     8401 gcttttcacg tttcaggaac ttcataaaaa attacgattc attttaccat gccggtgatg
     8461 ggctggcccg tgcaaccgtc gggaaactgg atattgagaa aattgcagac cgtgggcgca
     8521 acatccgtta tgttcatcag cgcatcgctg ctcccgttct ttattttcca accccaccag
     8581 tacatcggta cgtgcgtgtc gtagctccat gaagttccgt gcgtggttcc tttttttccg
     8641 aactcgctga accacgaagg ctgcagcacg acgcacacat cgccggaacg ctggaaattg
     8701 aacccgttcc ggataagctg ctgaggcttc tgtgtgtatt cgttcgattg caggtcggaa
     8761 gcggtcatgc acatttccac gcctttgaac cccatgatga acgaggcggt gaacttttcg
     8821 atctcgctct tgttcagtcc ttttttcgcg atgcccgcac ggtcgaggaa aacctggtcg
     8881 ttctcgtagc agagcacgag gctgtcgccg tagattttac gcatcacctt cttcagtgaa
     8941 tcggtcattt tcccttcgct gaaataacct gcgggaatat tattgtcttt gaggtactgc
     9001 ggattttcca cggcgccatg atcggcggtg aggaaaagca gcgtgttgtt cttccccaga
     9061 taattatcga ggaacgaaaa gaattccgcg agctctttgt cgagacgcag gtagcaatct
     9121 tccacttcca cgctgttggg tccgaactgg tggccgatat aatccgtgga gctgaagctg
     9181 acgcagagaa aatccgtcgc gcttcctttg ccgagatttt cgccgaggat cgtcgcctgc
     9241 gcgaattccc gcgtgagcga atttccgaac ggcgttgcgc ggatcatgcc cagcttcccg
     9301 ttcttgtcca tcagcagcgg aagatgatgc gggaaaacgg gtttgtcctc gcccgcatac
     9361 gtcgcttcgt atttgttgtc gtctgcaatg ctttcggtgt actgatcgat gggcagcagc
     9421 gtttcccagt ctttggaaag ataggattgc ggaagcttgc gtgcattgaa cgtgttcacc
     9481 cattcgggga gcgccgtcat gta
//
LOCUS       DASTSF010000396         2184 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1429040,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000396 DASTSF010000000
VERSION     DASTSF010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2184
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1429040"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..706
                     /locus_tag="VFU15_11265"
     CDS             <1..706
                     /locus_tag="VFU15_11265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718408.1"
                     /translation="GNNRKAAEYSSSWEKTGDMPAALLPYAFNVLQSVPQDAVLLTNG
                     EFDTFPLCQQQYANNVRPDVKLLCISFAQKSSNRAMLFRNAGLTLPGNDSTSAFDAAF
                     IMRVVSANPGKKIYLASTVSGKILAALQGDLYCTGLAFRYGDSPVNNLDFLKDNVGTK
                     MDLACLSKPPESKGVFDSNVSAQLNMNYVLPLTLAAKQYSLAGNTAKAEELNALARSI
                     GKAAGKEKTVNELIGG"
     gene            709..1311
                     /locus_tag="VFU15_11270"
     CDS             709..1311
                     /locus_tag="VFU15_11270"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU4718409.1"
                     /translation="MSLFRPSYEKESDELLMSRIVRGDSKAFAELYDRHSPAMFRYFH
                     RMLWKDRERAEDFTQDFFMKIVKTPDAFDVKRKFTTWMYSVAHNMCKNEYRRMEIRQG
                     PDFEKAAYAVEGDHGEHFGKRIDRKAFMERLSGELDKLDENQRTTFLLRYEENFSIRE
                     ISEILDCSEGTVKSRLFYTLKRLSTKLVEFAPEKNTVTES"
     gene            1308..>2184
                     /locus_tag="VFU15_11275"
     CDS             1308..>2184
                     /locus_tag="VFU15_11275"
                     /inference="COORDINATES: protein motif:HMM:NF024962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprB repeat-containing protein"
                     /protein_id="HEU4718410.1"
                     /translation="MKTGKQYQPEDMEALLAEKAFRELYDEEKKFVLEHLSSENEYEH
                     LRGMLLAIKGGGGTSGRGSDDLLPSPRVRHELLEEFEKERKRRALIWWNSAGLWLRNG
                     LRFDLPVIRIAFASLVLLIGVWMYFRFTSSSAPEKTIAGKDTSAKIVPLPSPQPVPLA
                     KNDSTAVKFATDKNENRSKEVPQEKVRRPVVNGNGGAPQNAVDRAQGNSADLANANHA
                     PGNNALRDSSGMKKDTASTPVIADAPAQQNFSADTTVNSMVLTSGSQVTCNGSSNGTA
                     NVTATGGTPSYNYAWN"
BASE COUNT          547 a          559 c          621 g          457 t
ORIGIN      
        1 cggcaacaac cggaaggcgg cggaatattc ttcttcctgg gaaaaaacag gcgacatgcc
       61 ggccgcgctt ctgccgtacg cgttcaacgt gctgcaatca gttccgcagg acgctgttct
      121 gctgacgaac ggggaattcg atacgttccc gctgtgccag cagcaatacg caaacaatgt
      181 ccgccccgat gtgaagctgc tctgcatttc gttcgcgcag aaatcatcca accgcgccat
      241 gctgttcagg aatgcaggac ttacactccc gggaaatgat tcgacgtccg ctttcgatgc
      301 cgcgttcatc atgcgggtcg tttccgctaa tcccgggaaa aagatctatc tcgcttcaac
      361 ggtgagcggg aaaatacttg ccgcgctgca gggcgatctg tactgcacgg gtctcgcttt
      421 ccgttacggt gattcgccgg tgaacaacct cgatttcctg aaagacaacg tgggtacgaa
      481 aatggatctt gcctgtcttt cgaaaccgcc ggaaagcaaa ggagttttcg acagcaacgt
      541 ttcagcgcag ctgaacatga attacgttct gccgctcacg ctggcggcga agcaatactc
      601 gctggcgggc aataccgcga aagcggaaga gctgaacgcg ttggcacgca gcatcggcaa
      661 agccgcggga aaagagaaaa cggtgaacga acttatcggc gggtgaaaat gtcgctgttc
      721 cggccttcat acgaaaagga aagtgatgag ctgctcatgt cgcgcattgt gcgtggcgac
      781 agcaaagcgt tcgcggagct gtacgaccgg cattccccgg cgatgttccg ttattttcac
      841 cgcatgctgt ggaaagaccg ggaacgcgca gaagatttta cccaggattt tttcatgaag
      901 atcgtgaaga cgccggacgc gtttgatgtg aagcggaaat tcacgacgtg gatgtacagc
      961 gtcgcgcaca acatgtgcaa gaacgaatac cggagaatgg agatccggca gggacctgat
     1021 ttcgagaaag cggcgtacgc ggtcgaagga gaccacggcg agcatttcgg aaaacgcatc
     1081 gatcgcaaag cattcatgga gcgtctctcg ggtgagctcg acaagctgga tgaaaaccag
     1141 cggaccacct tcctgctgcg ttatgaagag aatttttcca tccgcgagat cagcgagata
     1201 ctcgactgtt cggaaggaac ggtgaagtcg cgtttgtttt acacattgaa gcgcctttcc
     1261 acgaagctcg tggaatttgc gcccgaaaaa aatacggtta cggaatcatg aaaacaggaa
     1321 aacaatacca gcccgaggat atggaagcgc tcctggccga gaaagctttc cgcgagttgt
     1381 acgatgaaga gaaaaaattc gtcctcgagc atctttcatc tgaaaatgaa tacgagcacc
     1441 tgcgcggtat gctgctcgcg atcaaaggcg gcgggggaac gagcggccgc ggcagcgatg
     1501 atcttttgcc gtcgccgcgt gtgcgccatg agctgctgga agaatttgaa aaggaaagaa
     1561 agcggcgtgc gctcatctgg tggaacagcg ccggtctctg gctgaggaac ggcctgcgct
     1621 tcgacctgcc cgtcatccgc atcgcatttg cttcgcttgt ccttctcatc ggtgtgtgga
     1681 tgtatttccg gttcacgtct tcttctgcgc cggaaaaaac gatcgccgga aaagatactt
     1741 ctgcgaagat cgttcctctt ccttctccgc aacctgttcc tcttgcgaaa aatgattcaa
     1801 cggccgtgaa atttgctacg gataaaaatg agaacaggag caaggaggtt ccgcaggaga
     1861 aagtccgccg tcctgtcgtg aacggtaacg ggggtgctcc gcaaaatgca gtggaccggg
     1921 cgcagggcaa cagcgctgac cttgccaatg caaatcatgc gccgggaaat aacgcgctgc
     1981 gcgattcttc cgggatgaaa aaagatacag cttccactcc tgttattgcg gacgctcctg
     2041 cacagcagaa tttttccgcg gatacgacgg tgaattccat ggtattgacc agcgggagcc
     2101 aggtgacctg caacggaagc agcaatggaa cggcaaatgt tacggcaaca ggaggcactc
     2161 catcgtataa ttatgcgtgg aatt
//
LOCUS       DASTSF010000397        14771 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_946255,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000397 DASTSF010000000
VERSION     DASTSF010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 14771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14771
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_946255"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(467..655)
                     /locus_tag="VFU15_11280"
     CDS             complement(467..655)
                     /locus_tag="VFU15_11280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718411.1"
                     /translation="MADNNYREELSVQNREMQIMMIREKHPVYKDFNFEPYNDRKVAE
                     MFHVLVENYTKTEKETGA"
     gene            complement(798..1490)
                     /locus_tag="VFU15_11285"
     CDS             complement(798..1490)
                     /locus_tag="VFU15_11285"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEU4718412.1"
                     /translation="MNCLIIDDDEIARRSVQQCIDRTDFLSLAGSCASVTEAMKIIGS
                     QKIDLIFLDVEMPEMSGIDFLKTFREVPQIIFITGKKEYAAEAFDYDVTDFLLKPIDY
                     PRFLKAALKAKNMQEETQAGKDGEESAVYIKKEGNRLVRLPAAEILYVEALADYVNIF
                     TKDGRHTVLSTMKSIESKLPQKEFLRIHRSFIVRLDKVSEIEENAVVVNGKVIPVSRS
                     HKDDLFRRLKFL"
     gene            complement(1624..2544)
                     /gene="meaB"
                     /locus_tag="VFU15_11290"
     CDS             complement(1624..2544)
                     /gene="meaB"
                     /locus_tag="VFU15_11290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HEU4718413.1"
                     /translation="MTDVKDLVSRLLSGDFRALSRAITVVENDLEGTDEILSALPAGS
                     DVPLVGITGPPGAGKSTLINAIIHAYVSEGKKIAVIAVDPTSPFNFGSILGDRLRMSD
                     HFNNENVFIRSLATRGSLGGLSAKAIEVSDLVRAAGFDLVIVETVGVGQSEVEIAGLA
                     DTTVLVLVPESGDEVQVIKSGVMEIADVFAVNKADRPGANIFISNLHHLVHARAETGW
                     KPVIVRTVASRGEGIAELISAIKEHEKSADNPVKRKAVLLAQRAWKLMQEKSMKHIDR
                     EKMVREIEERLSSGDFHLFRFVQEKSAGTE"
     gene            complement(2585..3241)
                     /locus_tag="VFU15_11295"
     CDS             complement(2585..3241)
                     /locus_tag="VFU15_11295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718414.1"
                     /translation="MKKLLVLLLLMPFVLPAQETELQDIPRNEIGLNLFSLGTRTEPF
                     RYNMPYFSSAVASGISYKRSWDRNVLRTSATYSKELINYTTTGWEMTSQVHGTNATIG
                     LSCGYERRYGHWKVTPYSFADITYSYVNSLGHQNGLGGDIIWQFDNDYHYVTQGIGLQ
                     AGSGVRWQINEHLFLGYELSCGFLYNTMREKKPGVDRYYPEWDFVANPVRAFSFGVTF
                     "
     gene            3580..4869
                     /locus_tag="VFU15_11300"
     CDS             3580..4869
                     /locus_tag="VFU15_11300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014782271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P N-terminal domain-containing
                     protein"
                     /protein_id="HEU4718415.1"
                     /translation="MRYPQINNKLFIENRKRFTAKMKPRSLAVFNANDIQPTNADGTM
                     KFRQNSDLFYLSGVDQEETILVLFPDSPDPDHREVLFVRETNEHIAIWEGEKLTKSQA
                     TEVSGIKKVYWTHQFNSIFPGLAVEAENIYVNTNEHVRAVVEVETRDARFISWCRQKF
                     PAHQLMRSAPILHELRAIKSQIEIDLIGKACKLTEKGFRRLLGFVKPGVWEYEIEAEL
                     AHEFIRAGAGFAGYEPIIAAGKNSCVLHYNINNKQCKDGDILLMDFAAGYANYQSDLT
                     RTIPVNGKFTKRQKNVYNAVLRVMRYAMSRLVVGNTIQEYHKEVGAAMEEELLKLRLI
                     TKTDIKKQDPAWPAYKKYFMHGTSHYLGLDVHDVGSRYRKFTEGMVYTCEPGIYIPEE
                     SIGVRIENDILLTKKGPVDLMASIPIEADEIEALMKK"
     gene            4890..5747
                     /locus_tag="VFU15_11305"
     CDS             4890..5747
                     /locus_tag="VFU15_11305"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU4718416.1"
                     /translation="MQTIPAFSPKVPPVKFLISRGAKIRYSDSGKGRVIVLVHGFLES
                     LEVWYGNGFAQELAKKFRVVAMDLPGHGKSECLGYVQKMERMADVVKDVMDHLGLRRY
                     ILVGHSMGGYVSLAFAEKYPDNLRGLCLFHSTSLPDSEEKKLDRERAIRIVKRSPAKY
                     TNQLVVNLFALANVRYFRAEINWLRRMAAKTKKQAIVACLEGMKTRPGREMILRFAHF
                     PVMIIGGERDNVIPFSSLHDQVAHTEDGRFLALERTGHMGFIEAKKITLKKLSAFCRH
                     SYRKKISGS"
     gene            complement(5734..5973)
                     /locus_tag="VFU15_11310"
     CDS             complement(5734..5973)
                     /locus_tag="VFU15_11310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718417.1"
                     /translation="MNTVMLDFRVDGNELIVEYDQRTEKCLWSIFNLGGIELRNGLLK
                     GDAPHHISLERLSPDIYHLCIIDGDQLVNSKFRIR"
     gene            complement(6170..7873)
                     /locus_tag="VFU15_11315"
     CDS             complement(6170..7873)
                     /locus_tag="VFU15_11315"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEU4718418.1"
                     /translation="MKKILPALFFLSALPLAAQVSPTWGNDVLDINSVRAGVNSNGDL
                     FWNYINPQFQVPADSMTTTIIAGALWIGGLDTMNQLHLAAQTYRQTGNDFFNGPVMNS
                     ANYSTANDIAWAHVWKINKSTIDTFLMHFVNPALFPGYTIPASIMNWPGNGDVNQGQA
                     PQLAPFVDMNSDGYYDPNAGDYPCIKGDQSIFFIFNDDRSNHTETGGAKFGIELHGML
                     YGYKAPGTWLDSVVFVNYRLFNRTANDYNNLDLGSWADFDIGFAGDDYIGCDVARNIY
                     YGYNGDADDGSGPAPTTYTYGANPPAQGVVFLRGPEPSLNDGIDNDHDGQTDEANELC
                     AFNRFIYYNNDFSATGNPATSWDYYKYMSGRWKDSSLVTYGGNGYGGTTPAAFMFPGN
                     TDPAGIGTNYTPQPAWDEYTAGNSPGDRRGLGVSGPFPLPAHKEMCIDFAYVFGRGLN
                     GPLSSVAVMQNNSDSAVAFYNTTTPCLCVPNPLAVHEYHSITPQVYPNPSDGNVTVFW
                     QPQTPGAKLELYDMTGNVVLAMTINHAQTEISMEDLATGIYLLRITDGNKSGTAKIIK
                     E"
     gene            complement(8639..9388)
                     /locus_tag="VFU15_11320"
     CDS             complement(8639..9388)
                     /locus_tag="VFU15_11320"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEU4718419.1"
                     /translation="MINAVIVEDEKKSREVLEALVKANCPDVNIVGNADSVAAGVEVI
                     GKSKPQLVFLDIEMADGSGFDMLEKLGSGNFDVIFTTASDAHALKAIKYSAIDYLLKP
                     IDGEELKAAVEKIKAKHAETASMENLKFLLQNLRKPSEQYTKITLPTGNAYEIVNVKD
                     IIRCEADGSYTTFFLDGKKKLLVSASLKHYEDLLPPEDFIRVHHAYLVNMHHVVRYLK
                     ADGGYAVMSDGTQIEISRRKKEAFLQRLNKV"
     gene            complement(9422..12382)
                     /locus_tag="VFU15_11325"
     CDS             complement(9422..12382)
                     /locus_tag="VFU15_11325"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HEU4718420.1"
                     /translation="MLVNSARQKLYFLTPFFFALLFFSESFSQTLNFRQYSVDDGLPF
                     VQVFTIYQDSKGNLWSGGYGGLSSFDGVNFTNYAPRNGPPNHWVTSIAEDQRGQLWIG
                     TIEGASIFSNGTFQNFTVKDGLPDNYVNCLYRDDRNVFWIGTAKGICRYDGEKFIYDP
                     SEGPGKSSVLCIYGEPNTSRVWMGTSDGVYMYDNGKYTHYPMQTFSDDNVTAITRSAD
                     GKILAGTSDGVYRLEGDHFSILITQAGLEMPEVNALITDRSGITWIGADNGLFSYDGK
                     KFARYDISPDLNSEKVISLFIDYENSLWLGTHAGLFRYRGAGFVSFGPRDGLIGSFTY
                     GITQDGEGNIWVCSESKGVYKYDGKTFTLFGKHNGLSSEKANDCMYDSTLGILVGTNR
                     GLDVISGSQVKVFRRSDGLNCDTVNCIRRDRHGRIWLGGGKGITLFDNGKFTAYTIPS
                     SPENSFDVWSIFEDRTGRIWVGTYVGGLYIFDGKNFSDGKKLLGVKTDSYFDICEDKT
                     GKLYFATLDGIYIYNGKTTERISEQNGMSSELAYGMILDNESKYLWIGTNQGINRLDV
                     AAYDKDGSIVLTTFGKEEGFSGVESNTNGFYIDRDGKIWFGTVNGLICYSPKDFRTNI
                     APAKTSITGFRLFYQDTIIPQGAELEWDENNIAFEYAGICLTNPAKVRYRFMLAGFDQ
                     DWSPPTADRSAKYSNLPPGQYTFKVISCNNEGMWNEDPATYTFTILTPFWKKTWFWFL
                     LTLAAMGVLGSAILFRIRQIKYRERLESETKISLARNELKALRAQMNPHFVFNSLNSI
                     QHFILTNKSADAGKYLNKFARLMRVILNNSEKSLITVKEEIEYLTLYLGLEEMRFEGK
                     FSWSIDVPDDIDTDFIEIPAMLLQPYVENAILHGLTPKKEKGHLLIELRLKGNQLVCS
                     IIDDGIGRERAREMRRLSQQKDHKSLGMKITHDRLELINRLHGSHLSLTIIDLYHPGG
                     SAAGTRVDIFIPVS"
     gene            12957..14516
                     /locus_tag="VFU15_11330"
     CDS             12957..14516
                     /locus_tag="VFU15_11330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718421.1"
                     /translation="MSGKSSDQLHRLIHSLTKQEKRHFKIFAGRHTIGEQNNYVLIFD
                     IIDRMKEYDEELLIKKFKGETLLNNLSITKGRLYETILRSLDVFHHNSSVDAQLWKEL
                     HYAEILYKKTLYDQCSKRLKSARRLAEKFEKEAVLVQIHALEKSLAEKDNYSGMTDDD
                     ILRILEEDQKVADDIRTYNQLWNVKSRLFLVLNTKGRARGKEQLEDFRRLIDAPLQNK
                     SLDGLSVSARFMYHHTYSAYYFGIADAEKCHEHLAANVSLIESHTDIFRDEPNVYFGV
                     LTNLIYVCSRLKRYAEVLLNLKKLRALPEKLDSARNEDLDLKLFSSSYSLELSLYNSI
                     GHFENAMKLVPRIEEGLLRFEGRLSRLREAHFSISCAVACYGAGKIQQSLRWTTRLLN
                     DNAVGQNEWIYAVAQLFSLVLHLELDHRDLLPYVSRNVQRYLKSRNRSYQFENFFMAF
                     AEKSAKTTSEKEMIPLYTELWHNLESLRDDPFEQTAFEYFDFPAWAESKATGKSFAEM
                     VQEKSGATEEI"
     gene            14576..>14771
                     /locus_tag="VFU15_11335"
     CDS             14576..>14771
                     /locus_tag="VFU15_11335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718422.1"
                     /translation="MKKIFLLLFLFAIAPLLHAQPPTNAGEFTYLGKKYTANYDTGKH
                     QLTFSEEVSFEIYDDTGNCVK"
BASE COUNT         3475 a         3970 c         3820 g         3506 t
ORIGIN      
        1 gtcttcacat tcttccatcg taacaggctt catttaaatg atgtgatccc gatccgctgc
       61 gcttgcggga ttaattccgc cagcgcttcg aacttcgcgt tgctcgttct cagcgggcgg
      121 cgttttcagt gatgtgatcc cagttcgctg cgcttgcggg attaattccg ccagcgcttc
      181 gaacttcgcg ttgctcgttc tcagcgggcg acgttttcag agatgtgatc ccgatccgct
      241 gcgcttgcgg gattaattcc gccatcgctt cgaacttcgc gctgctcgtt ctcagggggc
      301 gacgttttca gtgatgtgat cccggttcgc tgcgctttcg ggattaattc cgccatcgct
      361 tcgaacttcg cgttgctcgt tctcagcggg cgtcattaaa aaggtctgtc cttacggaca
      421 gacccatacc gggatgcgca aaacagcata acccctacac ttttgtttac gcccccgttt
      481 ccttttcagt ctttgtgtaa ttttcaacga gcacatggaa catctccgcc actttcctgt
      541 cgttgtatgg ttcgaaattg aagtctttgt ataccgggtg cttttccctt atcatcatga
      601 tctgcatctc ccggttctgc acagaaagct cctccctgta attattgtct gccattagtt
      661 tcgccctgcc atatttttcg gctacaaaaa tggatcaaaa aaaacgcagt aaaaaagaat
      721 tacgatactt ggccttattc acccgacgaa cagttttcag agggagatga acaagaggaa
      781 agaaatccct aaaccgatta cagaaatttc aggcgacgga aaagatcgtc tttgtgcgag
      841 cggctgacgg gaataacttt cccattgacg acaactgcat tctcttcgat ctcggaaact
      901 ttatcgagcc gcacaatgaa cgagcgatgg atgcggagaa attctttctg gggcagcttg
      961 gattcgatcg acttcatcgt tgaaagaacg gtgtgacggc cgtctttcgt gaaaatattc
     1021 acgtaatcgg cgagcgcttc cacatagagt atctcggctg cagggagacg gacaagacgg
     1081 ttgccttctt tcttgatata taccgcgctt tcctcgccgt cctttccggc ctgcgtttct
     1141 tcctgcatgt tcttcgcttt gagcgccgct ttcaggaaac gcgggtaatc gatcggcttg
     1201 agaaggaagt ccgtcacgtc gtaatcgaac gcttcagcgg cgtattcctt tttgcccgtt
     1261 atgaaaatga tctgcgggac ctcgcggaat gttttcagga aatctatgcc gctcatttcc
     1321 ggcatctcca cgtcgagaaa gatcaggtcg atcttctggc tgccgatgat cttcatcgct
     1381 tccgtcacgc tcgcgcacga tccggcaagc gaaaggaaat ccgtcctgtc gatgcattgc
     1441 tgcacgcttc tccgtgcaat ttcgtcgtca tcaataatga ggcaattcat gggttgttac
     1501 ggccaaaata gaccataagg cattaaaagt caaatttata ttatggtgat ttacgttaaa
     1561 atcactcgtt cagatcggcc gtttttttaa acttttccgc tgcctccccg tgccggaaca
     1621 tatttactct gttccggcgc ttttttcctg cacgaaacgg aaaaggtgga agtccccgga
     1681 agaaagacgt tcctctattt cccgcaccat tttttcccgg tcgatgtgct tcatgctttt
     1741 ttcctgcatc agcttccagg cgcgctgggc aaggagaacg gctttccgct ttaccgggtt
     1801 gtccgccgat ttttcatgct cttttatcgc gctgatcagc tcggcaattc cttcgccccg
     1861 ggaggcaacg gtccgcacga tgaccggctt ccacccggtt tccgcacgcg cgtgcacgag
     1921 atgatgaaga ttgctgatga aaatattcgc gccgggacgg tccgctttgt tcacggcaaa
     1981 aacatccgcg atctccatca cgcccgattt gatcacctgc acttcgtcgc ccgattctgg
     2041 aacgagaacg agaacggtcg tgtccgcaag tcccgcgatc tccacttccg actggccgac
     2101 gccgacggtt tcaacgatga caagatcgaa cccggcggcc cgcacgagat cgctcacctc
     2161 gatcgctttt gcagaaagtc cgcccagcga accgcgcgtg gcgagcgagc ggataaaaac
     2221 attttcattg ttgaaatgat ccgacatgcg cagcctgtcg ccgagaatgg agccgaaatt
     2281 gaacggcgac gtcgggtcga ccgcgatcac cgcgatcttt tttccttcgg aaacatacgc
     2341 gtgaatgatc gcattgatga gcgtgctttt ccctgctccc ggcggacccg taatgcccac
     2401 cagtggaacg tcgctgcccg ctggaagcgc cgaaagaatt tcatccgtcc cttcaagatc
     2461 gttttccacg accgtgatcg cacgcgaaag cgcacggaag tcgccggaaa gcagccggga
     2521 aacgagatcc ttgacgtcgg tcatcaggga tcaaaggtaa aataaagcga atggaaagct
     2581 ttcgtcagaa cgtcacgccg aacgagaaag cacgcacagg gttggccaca aaatcccatt
     2641 cgggataata cctgtcaacg ccgggtttct tttcccgcat cgtattgtac aggaaaccac
     2701 agctcagctc gtaaccgaga aaaagatgct cattgatctg ccaccgaact ccgctgccgg
     2761 cctgcagtcc gattccctgc gtcacataat ggtaatcatt atcgaattgc cagatgatat
     2821 cgccacccag gccattttgg tggccgagcg agttcacata actgtacgtg atgtcggcga
     2881 aagaatacgg tgtcactttc cagtggccat aacggcgctc gtaaccgcag ctcagcccga
     2941 tcgtcgcatt ggtgccatga acctgtgaag tcatttccca acccgtggtc gtgtaattga
     3001 tgagctcctt cgaatacgta gcggacgtcc ggagaacgtt cctgtcccac gaccgcttgt
     3061 acgaaatacc cgacgcgacc gcggatgaaa aataaggcat attgtacctg aagggttccg
     3121 tacgtgtgcc aagggaaaaa aggttcaggc cgatctcatt gcggggaatg tcctgcagct
     3181 ccgtttcctg tgcaggaaga acgaacggca tcagaagaag caggacaaga agttttttca
     3241 tcgggacagg ctttaagggc gttttgcgga gacaggaact caaagttaag tcattgcacg
     3301 ggttgaacgg atgcaaaaat ccggcgaaca gttcggcgtt tataagcaga cgtgccggcc
     3361 gctgaaacga tgccggcaac cggacgaaaa aattgaaaat atcctccacg gttgctgcgg
     3421 ttatcttccg gaacgcaggg atgaacggcg ataatgctac ggcagctgcc ggttttttca
     3481 ttcgggcgcg gggttttgtc aaatgcagga aaaggatgcc tgttttcatt tcgtgtccac
     3541 acggtttatc tatctttatt cgttccaaat ttcattctta tgcgctatcc tcagatcaac
     3601 aacaagctct tcattgaaaa ccgcaaacgc ttcaccgcga agatgaagcc gagatcactc
     3661 gctgttttca acgccaacga tattcagccc acgaacgcgg acggaacgat gaaattccgg
     3721 cagaacagcg acctgttcta cctcagcggc gtcgaccagg aagaaacgat cctcgtgctc
     3781 ttccccgatt ctcccgatcc ggatcaccgc gaagttctct ttgtgcgcga aacgaacgag
     3841 cacatcgcga tctgggaagg agaaaagctg acgaaatcgc aggcgacgga agtttcggga
     3901 attaaaaaag tgtactggac gcaccagttc aactcaatat tccccggtct cgccgttgaa
     3961 gcggaaaaca tttatgtaaa tacgaatgag cacgtgcgtg cggtggtgga agtggaaacg
     4021 cgtgacgccc gtttcatcag ctggtgcagg caaaaatttc ctgcgcacca gcttatgcgc
     4081 agcgcgccca tcctgcatga gctgcgcgcg atcaaatcac agatcgagat cgatctcatc
     4141 ggcaaggcct gcaagctcac ggaaaaaggt ttccgccgcc tgctcggttt cgtgaagccc
     4201 ggcgtatggg aatacgagat cgaagcggag cttgcgcacg agttcatccg cgcaggcgca
     4261 ggcttcgccg gttatgaacc gatcatcgcg gcgggaaaaa attcctgcgt gctgcattac
     4321 aacatcaaca acaagcaatg caaagacgga gatatcctgc tgatggattt cgccgcaggc
     4381 tatgccaatt accagagcga cctgacacgc accattcccg tgaacggaaa attcacgaag
     4441 cgacagaaga acgtgtacaa tgccgttctc cgcgtgatgc gttacgcgat gtcgcggctc
     4501 gtcgtcggca atacgataca ggaataccac aaggaggtcg gtgcggcgat ggaagaagaa
     4561 ttgctgaagc tgcgcctcat cacgaaaacc gacattaaaa aacaggatcc agcctggccg
     4621 gcctacaaaa aatatttcat gcacggcact tcgcactacc tcggtctcga cgtgcatgac
     4681 gtgggcagcc gctaccgcaa gttcaccgaa ggaatggttt acacctgcga gccgggcatt
     4741 tacatccccg aagaaagcat cggcgtgcgt atcgagaatg atattctcct tacgaaaaaa
     4801 ggccccgtcg atctcatggc gtcgattccc atcgaagcgg acgagatcga agcgctgatg
     4861 aaaaaataag tccgcacgtt ttcgctttca tgcagacgat ccctgcattc agcccgaaag
     4921 ttccgccggt gaaatttctc atttcgcgcg gcgcgaagat ccgttacagc gattcgggga
     4981 aaggacgcgt gatcgttctc gtgcacggct ttctcgaatc gctggaagtg tggtacggca
     5041 acgggtttgc gcaggagctc gcgaaaaaat tccgcgtcgt cgccatggac cttccgggtc
     5101 acgggaaatc ggaatgcctg ggttatgtgc agaaaatgga acgcatggcg gacgtggtga
     5161 aggacgtgat ggatcatctc gggctgcgcc gttatattct cgtgggtcat tcgatgggcg
     5221 gatacgtttc gctcgcgttc gctgaaaaat atcccgacaa cctgcgcgga ctttgtcttt
     5281 ttcattccac gtcgctgccc gacagcgaag aaaaaaaact cgaccgcgag cgcgcgatcc
     5341 gcatcgtgaa acgcagtccg gcgaaataca ccaaccagct cgtcgtgaat ttattcgccc
     5401 tggcgaacgt acgttatttc cgcgcggaaa taaactggct gcggcgcatg gcggcgaaaa
     5461 cgaaaaagca ggccatcgtc gcctgcctcg aaggaatgaa gacacgtccc ggccgggaaa
     5521 tgatcctgcg cttcgcacat ttccccgtca tgatcattgg cggcgagcgc gacaacgtca
     5581 ttcctttttc atcgctgcac gaccaggtcg cccatacgga agacgggcgg ttcctcgccc
     5641 tcgaacgcac cgggcacatg ggatttatcg aagccaaaaa aataacgctg aaaaaacttt
     5701 ccgcgttctg ccgtcacagc taccggaaaa agatcagcgg atcctgaact tcgaattcac
     5761 gagctggtcg ccgtcgatga tgcagaggtg gtagatgtcg ggggaaagac gttcgaggga
     5821 aatatgatgc ggcgcatccc ctttcagcag gccgttgcgc agctcgatcc cgcccaggtt
     5881 gaaaatgctc cacaggcatt tttcggttct ctggtcgtat tcaacaatca gctcgtttcc
     5941 gtcaacgcgg aagtccagca tcactgtatt catagcaaat aagattattc ctttccaggg
     6001 ggattacttc tttaacgctg aggttgcacg caggtttaat cttttcgatc aatcagggta
     6061 atccggggac gaagttccgt ctcttttcag gccggcacct tttccctgtt tttatggaat
     6121 aaaaaaggct ctgtgaaagt tcacagagcc gaaaggagga attaacgatt tattctttta
     6181 tgatctttgc cgttcccgac ttgtttccgt ccgtgatccg cagcaggtaa attcccgtcg
     6241 ccagatcttc catgctgatc tccgtctgcg cgtggttgat cgtcatcgcc agcacgacgt
     6301 tgccggtcat gtcgtacagc tccagcttcg caccgggtgt ttgcggctgc cagaagacag
     6361 taacgttccc gtccgaagga ttcggataca cctgcggcgt gatcgaatga tattcgtgga
     6421 cagcgagcgg gttgggcacg cacaggcatg gtgttgtcgt gttgtaaaac gcaaccgcgc
     6481 tgtcggaatt attctgcatc acggcaacgg aagacagcgg accatttagt cctctcccga
     6541 aaacatacgc gaagtcgatg cacatttcct tgtgcgccgg aagagggaac ggcccggaca
     6601 ctccaagacc cctgcgatcg ccgggtgagt tacccgcagt atattcatcc cacgccggct
     6661 gcggcgtgta attggttccg attcccgccg gatccgtatt gccgggaaac atgaatgccg
     6721 caggcgtcgt tccaccgtaa ccatttcctc cgtaggttac aagcgagctg tccttccagc
     6781 gtcccgacat gtacttgtaa taatcccagg aagtagcggg attcccggtc gcggaaaaat
     6841 cattgttgta ataaatgaac ctgttgaatg cgcagagctc gttcgcttca tccgtctgcc
     6901 cgtcgtgatc attatcgata ccatcattga gtgacggttc gggcccgcgg agaaaaacaa
     6961 caccctgtgc cggcggattt gcaccgtatg tgtacgtggt cggagcagga ccggatccgt
     7021 cgtccgcatc tccgttatag ccataataaa tattacgcgc gacgtcgcac ccgatgtaat
     7081 cgtccccggc aaacccgatg tcgaaatcgg cccacgaacc gaggtcaaga ttgttatagt
     7141 cgttcgccgt acggttgaac agccggtaat tcacaaacac gacgctgtcg agccacgtgc
     7201 cgggcgcttt gtacccgtac agcatgccat gcagctcgat gccgaatttc gctccgccgg
     7261 tttcggtgtg attcgagcga tcgtcattga aaataaaaaa gatcgactgg tcacctttga
     7321 tgcagggata atcgccggca ttcggatcgt aatacccgtc ggaattcata tccacgaacg
     7381 gggccagctg tggcgcctgt ccctggttta cgtcgccgtt gccgggccag ttcatgatcg
     7441 aagccgggat ggtgtaaccg ggaaaaagtg cagggttgac aaaatgcatc aggaacgtat
     7501 cgatggtcga cttgttgatc ttccacacat gcgcccaggc gatgtcattg gccgtggaat
     7561 agttcgcgga attcattaca ggtccgttga agaaatcatt ccccgtctgg cgatacgttt
     7621 gcgcagcgag gtgaagctgg ttcatcgtat caagtccacc gatccataac gcgcctgcga
     7681 tgatcgtggt ggtcatgctg tcggccggaa cctggaactg gggattgatg taattccaga
     7741 aaagatcccc gttggaattc acgcctgcgc gcacgctgtt gatgtcgagc acgtcgttgc
     7801 cccacgtagg agaaacctgc gcagcgagcg gaagagcgga aagaaaaaag agtgctggta
     7861 aaattttttt catggcgttt tttttaatgg gtgaggtggg gatgctcatg cggaataaac
     7921 gtaggtcttc gctatgaaag ggccaatctt tttttccgca tgattataca tgtccaaaaa
     7981 aaacgtcccg gcaaaaaccg ggacgtgtac tctgtatagt gaaggccttt cggcctggag
     8041 gactttgaaa aaaccccggt tcacgctaag ccttattcgg cccggcagaa ccggggtccc
     8101 acacacactc aggaaaatat aacgtgtaat acgttgtaga gaatgaatcc gatgcaggct
     8161 actccgaaaa gtatgcccat ggtgccgaga ccgtcaatat gataagcgtg tcccttgcga
     8221 tgctctgtct ttttcatggc acattgattt taattgttca acttatgttt tgtccaccgc
     8281 gatgatggat cgacttctgc ttttacctgt ataacccgcg cccgctgaat agatacgttt
     8341 gcctggtaaa agagccgggt gaaccagatg gtcggtgcaa catccccgaa tacacctgcc
     8401 aaccggtatc accctttggc tcagggccag tggctctttt tcgttgagta tctttatttc
     8461 aatgagcgtt taccgggtgg aaccagcgaa aagtcctgtg ctgatttcca ctcaatatta
     8521 cgcatgaaac aaaaggaagg atagccacat ttaacaggtg gcgaacaatg caaagcaatc
     8581 gggaaagaga ggaaggtaac tggctgcgcg gaaaccggcg gctccgtgca cggttctgct
     8641 aaaccttgtt caggcgctgc aggaacgctt ctttcttgcg tctcgagatc tcgatctgcg
     8701 taccgtcgga cattaccgcg tagccgccgt cggctttcag gtagcgcacg acgtgatgca
     8761 tgttgacgag atacgcgtgg tgcacgcgga tgaaatcttc cgggggaaga aggtcttcgt
     8821 aatgcttgag gctcgccgaa acgagcagct tctttttacc gtcgaggaaa aacgtggtgt
     8881 agctgccgtc cgcttcgcag cggataatgt ccttcacgtt cacaatctcg tatgcgttcc
     8941 ccgtgggcag ggtgatcttc gtatactgtt cgctgggctt gcggaggttc tgcaggagaa
     9001 atttcaggtt ctccatgctc gcggtttccg catgctttgc tttgatcttt tccaccgccg
     9061 ctttcagctc ttccccatcg atcggtttca gcaggtagtc gatggcgctg tacttgatcg
     9121 ctttcagcgc atgcgcatcg gacgcggtcg tgaagatcac atcgaaattt cccgagccga
     9181 gcttttccag catgtcgaag ccgcttccat ccgccatttc gatgtcgaga aaaacgagct
     9241 gcggtttcga tttcccgatc acctcaacgc ccgcggcaac cgaatcggca ttgcccacga
     9301 tgttcacatc gggacaattc gcttttacaa gcgcttcgag cacttccctg cttttctttt
     9361 cgtcttctac gattaccgcg ttgatcataa ggattgtttt tgggattgat atcctgccaa
     9421 attaggaaac cggtatgaat atatcaactc tggttcccgc tgcgctgccg ccgggatgat
     9481 aaaggtcgat gatggtaaga ctgaggtgcg atccgtgcag gcggttgatc agctcgagcc
     9541 tgtcgtgcgt gatcttcatg ccgagcgatt tatggtcttt ctgctgcgaa agccggcgca
     9601 tctcccgcgc gcgctcgcgc ccgatgccgt cgtcgatgat gctgcacacg agctggtttc
     9661 ccttcagccg cagctcgatc agcaggtgac ctttttcttt tttcggcgtc agcccgtgga
     9721 ggatggcatt ctccacataa ggctgcagca gcatggccgg tatctcgatg aaatcggtgt
     9781 cgatgtcatc cggcacgtcg atgctccagg aaaattttcc ttcgaaacgc atttcttcaa
     9841 gaccgagata aagcgtcagg tattcgatct cctccttcac ggtgatcagc gatttttcgg
     9901 aattgttcag gatcacgcgc atcagccgcg caaacttgtt caggtatttt cctgcgtcgg
     9961 ctgatttgtt cgtaagaata aaatgctgga tggaattgag cgaattgaaa acgaaatgcg
    10021 ggttcatctg cgcgcgcaag gctttcagct cattccgggc gagagaaatt tttgtttccg
    10081 attcgaggcg ttcgcggtat ttgatctggc ggatgcggaa caggatggcc gaaccgagaa
    10141 cgcccatcgc cgcaagcgta agcaggaacc agaaccaggt ctttttccag aacggcgtga
    10201 ggatcgtgaa cgtatacgtc gccgggtctt cgttccacat gccttcgttg ttgcagctga
    10261 tgaccttgaa cgtgtattgc cccggcggca ggttggaata ttttgcgctg cggtcggcgg
    10321 tcggcggcga ccagtcctgg tcgaaacccg cgagcatgaa acggtaacgc actttcgccg
    10381 ggttcgtcag gcaaatgccg gcatattcga atgcgatgtt gttctcgtcc cattcgagct
    10441 ccgctccctg cgggatgatc gtgtcctggt aaaaaagccg gaagccggtg atactggttt
    10501 tcgccggcgc aatattcgtg cggaaatctt tcggcgaata gcagatgagc ccgttcaccg
    10561 taccgaacca gatcttcccg tcccggtcga tgtaaaaccc gttcgtgttc gattccacgc
    10621 ctgaaaaacc ttcttctttc ccgaacgtgg tgagcacgat gctcccgtct ttatcatacg
    10681 cagcgacatc cagccggttg atcccctggt tcgtaccgat ccacaggtat ttgctttcgt
    10741 tgtcgaggat cattccatac gcaagctccg agctcattcc gttctgctcc gagatacgtt
    10801 ccgttgtttt tccgttgtaa atgtatatgc cgtcgagcgt ggcaaaatac agcttgccgg
    10861 tcttgtcctc gcagatgtcg aaatacgagt ccgtcttcac gccgaggagc tttttcccgt
    10921 cggaaaaatt cttcccgtcg aaaatgtaga gcccgcccac gtatgtcccc acccagatgc
    10981 ggcccgtacg gtcttcaaaa atgctccaca cgtcgaatga attttcaggt gaggacggga
    11041 tcgtgtacgc cgtgaatttc ccgttgtcga aaagcgtgat cccttttccg ccgccgagcc
    11101 agatcctccc gtgccggtcg cggcgtatgc aattcaccgt gtcgcagttc agcccgtcgc
    11161 ttcgccggaa tacttttacc tggctgccgg aaataacgtc gagtccgcgg ttcgttccca
    11221 caaggatgcc gagcgtggaa tcatacatgc agtcgttcgc tttttcggat gaaagtccgt
    11281 tgtgtttccc gaacaaagtg aatgttttgc cgtcgtattt gtaaacacct ttcgattccg
    11341 agcacaccca gatatttcct tcgccatcct gtgtaatgcc gtacgtgaac gaaccgataa
    11401 gcccgtcgcg cggcccgaaa ctgacgaacc cggctccgcg ataacggaac agcccggcgt
    11461 gcgtaccgag ccagaggctg ttctcataat caatgaaaag gctgatcact ttttccgaat
    11521 tcaggtcggg actgatgtcg tagcgcgcga atttttttcc gtcgtaggag aacagcccgt
    11581 tgtccgcacc gatccaggtg atgccgctgc ggtcggtgat cagcgcattc acctccggca
    11641 tttccagccc ggcctgcgtg atgagaatgg aaaaatgatc tccttccagg cggtaaacgc
    11701 cgtcggaagt gccggcaagg atcttcccgt cggcggagcg ggtgatcgct gttacgttgt
    11761 cgtcggagaa cgtctgcatc gggtaatgcg tgtatttccc gttgtcgtac atgtacacgc
    11821 cgtccgacgt gcccatccac acgcgcgaag tattcggctc gccgtaaatg cagagcacag
    11881 agctttttcc gggtccttcg ctcggatcgt aaatgaattt ttcgccgtca taacggcaga
    11941 tcccttttgc cgttccgatc cagaaaacgt tccggtcgtc acggtacagg cagttcacgt
    12001 aattatcggg cagtccatcc ttcacggtaa aattctggaa cgtgccgttg ctgaaaatgg
    12061 aagcgccttc gatcgtcccg atccataatt gtccgcgctg gtcttccgcg atcgacgtca
    12121 cccagtggtt gggggggccg ttgcgcggcg cataattcgt gaagttcacg ccatcgaagc
    12181 tgctgagtcc gccgtagccg cccgaccata aattcccttt gctgtcctgg taaatggtga
    12241 agacctgtac gaacggaagt ccgtcatcga ccgaatactg gcggaagttg agcgtctgcg
    12301 aaaatgattc ggagaaaaaa aggagcgcga aaaaaaacgg tgtgagaaaa tataatttct
    12361 gcctggcgct gttcacgagc ataagtaaag ttaacggttc ctcccggaaa gaaatccggt
    12421 gcgttggcaa acccggaaca gggcgggagc tttcgtaccg ggtgaagaga aaatttattt
    12481 catttcggaa atgacaaccc ggctttcgta aaaaaaccgg ggtccggcca acatcccctt
    12541 tgaaaccgga cccacggcgg atcgcatgaa atttaccaat acgtgacaaa gctatacgcc
    12601 gaaggaaggt tcctcaaatc acagttccgg gttgttttac atctcaagat ccatgcagtt
    12661 tgttctacct ggctattttc caggatgttg aacctcaaaa aatatgctga tcaagcccgg
    12721 tttccacttt ttttcatctt tatacatgtc cagatgtgtt tacgggcgaa tccaaaccat
    12781 cctgtgcagt tgcaaaagca gcttcgatta attaacttgt tggaaatgga aatggctgca
    12841 ccacctgcga ataaagagag acaaaagctt ctgctttttt tgaatgaaag gattcccggg
    12901 ataaaacgag tgcaggtcat tgctacgcta acccccaaaa acacgcatct ttaaacatgt
    12961 ccggcaaatc ttccgaccag cttcaccgcc tcatccactc gctcaccaaa caggaaaaaa
    13021 ggcatttcaa aattttcgcc gggcgtcata ccatcggtga gcagaacaac tacgtcctga
    13081 tcttcgacat catcgacagg atgaaggaat acgatgaaga gctgctcatt aagaaattca
    13141 aaggcgaaac gctcctcaac aacctcagca tcacgaaagg acgtttatat gaaacgatcc
    13201 tccgcagcct cgatgttttc caccacaaca gctccgtcga tgcgcagctg tggaaagagc
    13261 tgcattacgc cgagatcctg tacaagaaaa cgctgtacga tcaatgctcg aagcggctga
    13321 aaagcgcgcg gaggctcgct gaaaaattcg agaaggaggc cgtgctcgtc cagatacacg
    13381 cgctggaaaa atcgctggcg gaaaaagaca attattccgg catgacggac gatgatattc
    13441 tccgcatcct ggaagaagac cagaaagtgg cggacgacat acgcacgtac aaccagctgt
    13501 ggaatgtcaa aagccgcctg ttcctcgtgc tgaacacgaa aggacgcgca cgcggaaaag
    13561 aacagctcga agatttccgc cgcctcatcg atgcgccgct gcagaacaaa tcgctcgacg
    13621 gcctgagcgt aagcgcacgt ttcatgtacc atcacacgta cagcgcgtat tatttcggca
    13681 tcgccgatgc ggaaaaatgc cacgagcatc ttgccgccaa cgtcagcctc atcgaatcgc
    13741 acacggatat tttccgcgac gagccgaacg tgtatttcgg cgtgctgacg aaccttattt
    13801 atgtctgcag ccggctcaag cgctacgcgg aagtgttgct caacctgaaa aagcttcgcg
    13861 cattgccgga aaagctcgac agcgcacgca acgaggacct cgacctgaaa ttattttcca
    13921 gctcctacag cctcgagctt tcgctctaca attcgatcgg gcattttgag aatgcgatga
    13981 agctggtccc gcgcatcgag gaaggattgc tccgtttcga gggaaggctg agccgcctgc
    14041 gtgaagcgca tttttccatc agctgcgccg tggcctgcta cggtgcgggg aagatacagc
    14101 agtcgctgcg ctggaccacg cgcctgctga acgacaacgc cgtgggacag aacgaatgga
    14161 tctacgcggt ggcacaactg ttctcgctgg tgcttcacct cgagctcgat caccgcgacc
    14221 tgcttccgta cgtcagccgg aatgtgcagc gttacctgaa atcacggaac cgctcgtacc
    14281 agttcgagaa tttcttcatg gcgttcgcgg aaaaatcagc gaagaccacc agtgaaaaag
    14341 agatgatccc gctgtacacg gagctgtggc ataatctcga atcgctcagg gacgatccgt
    14401 tcgaacagac cgcgttcgag tatttcgatt ttcccgcgtg ggcggaaagc aaagccaccg
    14461 ggaaatcatt tgccgaaatg gtgcaggaaa aatcgggagc gacggaagaa atctgagctc
    14521 atcttcacga aagattaacg cggcctccct acttttgcac aacaacctgc cgtccatgaa
    14581 aaaaatcttc ctgcttcttt ttcttttcgc gatcgcgccg ctgcttcatg cgcagcctcc
    14641 cacgaatgca ggtgaattca cttacctcgg gaaaaaatac accgcgaatt acgacacggg
    14701 caagcaccag ctgacgttca gcgaagaagt ttcattcgag atctacgacg acacgggcaa
    14761 ctgcgtcaag c
//
LOCUS       DASTSF010000398        17102 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1644159,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000398 DASTSF010000000
VERSION     DASTSF010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 17102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17102
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1644159"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..789
                     /locus_tag="VFU15_11340"
     CDS             <1..789
                     /locus_tag="VFU15_11340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015809876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HEU4718423.1"
                     /translation="PRIKPPFPAVQGVWARPTVVNNVETIAATVPIVNMGGEEYAKIG
                     IGKSTGTKLISACGHINKPGVYEIDLGLPVEEFLYSENYCGGIRNGKKLKAVVAGGSS
                     VPILPAELFLKTAKGEPRLMSYESLADGGFQTGTMLGSGGFIVMDETTSVVKNLWNFT
                     RFYHHESCGQCSPCREGTGWMEKILHRILDGHGHPGDVDLLFDIQSKIDGKTICPLGD
                     AAAWPVASAIRHFRAEFEEYIKNPSSIKDQKHYYNLRNQAAPVA"
     gene            825..1814
                     /locus_tag="VFU15_11345"
     CDS             825..1814
                     /locus_tag="VFU15_11345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013664882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEU4718424.1"
                     /translation="MPKVTIDGHEIEVPKGTTILQAARMIGKHVAPPAMCYYSKLKTS
                     GGYCRTCIVRVTKMNSQNPNPMPKPVASCRTEVMDGMEVVNFTSPEVLEARRGVVEFL
                     LLNHPLDCPICDQAGECHLQDLGYENGAAKTRYDFERRTFEKIDIGPYVQLHMTRCIL
                     CYRCVKVAEQLTDHRVHGVLNRGDVAEISTFVKNSIDNEFSGNVIDVCPVGALTDKTF
                     RFKSRVWFVKPVDAHRNCTKCSGKVRLWFKGADVLRVTARKDQWGEVEDWICNDCRFN
                     HKKVSDWNIEGPSHIDHHSVISANHYELVKLKLDTGKQLGKLKGKKHKESSSH"
     gene            1818..2864
                     /gene="nuoH"
                     /locus_tag="VFU15_11350"
     CDS             1818..2864
                     /gene="nuoH"
                     /locus_tag="VFU15_11350"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008246355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HEU4718425.1"
                     /translation="MTTAFIIYKIILVLLVFAVSLGIAAYSTYAERKIAAFLQDRLGP
                     NRAGPFGILQPIADGIKMFMKEEIIPNVSNKFLFVLGPAIGMLTALMTGAVIPWGPNI
                     MWHGESLPLQVTDVNIGVLYLLGVVSIGVYGIMIGGWASNNKYSLLGALRASSQMISY
                     EVAMGIAIVTLIMMTGTLQTSAIAAEQHSFWNGHYFSWNFFKNPLTFLIFFICALAET
                     NRTPFDLPECETELVGGYHTEYSSMKLGFYLFAEYVNMFISSAVMSLFFFGGYNFPFM
                     DKISDPNIVALIGTLVMFAKTLFFVFVFMWIRWTIPRFRYDQLMNLGWKILIPLSLLN
                     LIGTGLGMWGMGKL"
     gene            2907..3518
                     /locus_tag="VFU15_11355"
     CDS             2907..3518
                     /locus_tag="VFU15_11355"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013633976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HEU4718426.1"
                     /translation="MQSLTNRSKVVVKKEMSFTEKLYLPAVLNGMRITLSHLFKKKAT
                     ISYPEKERPRSSVYRGEHVLKRDENGAERCTACGLCAVACPAEAITMVAAERKKGEEN
                     LYREEKYAAVYEINMLRCIFCGLCEEACPKEAIFLTDRIVPTEFERRDFVYGKDKLVE
                     PVDKRVDVTKRQTAAVLEFKKHPEFAHNKLWDPSRSLKTKKAH"
     gene            3534..4043
                     /locus_tag="VFU15_11360"
     CDS             3534..4043
                     /locus_tag="VFU15_11360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEU4718427.1"
                     /translation="MNLTITEVLFFVLSAIALISGACVIFSRNPVYSVLYLIVTFFAI
                     AGHYILLNAQFLAVVHIIVYAGAIMVLFLFVIMMLNLNKETEPHKPAWIKIAAVVAGG
                     TLMLTLVGALKGADTIALKQPNDTQIGLVTRLGHVLFTDFIVPFEISSVLFLAAMVGA
                     VMLGKKNIT"
     gene            4045..4389
                     /gene="nuoK"
                     /locus_tag="VFU15_11365"
     CDS             4045..4389
                     /gene="nuoK"
                     /locus_tag="VFU15_11365"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013664886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEU4718428.1"
                     /translation="MSTMLLDAGHSAIQVIPISWYVTFSAVLFGMGVLGVLYRRNAII
                     ILMCIEMMLNAVNLLLVAFSAQHSDPRAQVFVFFVMAVAAAEVAVGLAILMIIYRNTK
                     TTDIDALNKLKG"
     gene            4397..6292
                     /gene="nuoL"
                     /locus_tag="VFU15_11370"
     CDS             4397..6292
                     /gene="nuoL"
                     /locus_tag="VFU15_11370"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020597412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEU4718429.1"
                     /translation="MIQLAWLIPVLPLLGFAIIGLGGKKLSKGQISLVGCGSVLLAFL
                     LSLGIFFQLHGQSEVVKVASWFATGRLSVDFSFLIDPLSVLFMLIITGVGFLIHLYST
                     GYMHDDDGYHRFFSYMNLFVFFMLLLVMGSNYILMFVGWEGVGLCSYLLIGFWFKNTE
                     YNNAAKKAFIVNRIGDLGFLLGMILIYVTFKSLDFSVVFKGVHDNAYNQSIYNTIGIL
                     LFIGACGKSAQIPLYTWLPDAMAGPTPVSALIHAATMVTSGIYMITRSSVIFINAPVA
                     SEVVALIGVLTAVFAATIGLFQNDIKKVLAYSTVSQLGYMFLGLGVGAFTGGVFHVMT
                     HAFFKALLFLCAGSVIHAMSGEQDIRRMGGLKKALPITHLTFLIGTLAISGLPPFSGF
                     FSKDEILGHVYEHEGSGKLYWVLGVIGSMLTAFYMFRLYFLTFHGKFRGTHEQEHHLH
                     ESPKSMTVPLMILAGLAAVAGFLGIPNAHQHLLYNFLEPGYGYAKTWIEGPSHFGGEE
                     LKLMLIAIASAGAAIYLAFMIYRKNNVLPKEKEEDMPKIQRVIFHKYYVDEFYDAVIR
                     KPLDWLSEMFYKFFELKFIDGIVNGIGSGVKWMGSMARYVQTGSIGFYLFAMSLAVML
                     IFMIKFF"
     gene            6309..7826
                     /locus_tag="VFU15_11375"
     CDS             6309..7826
                     /locus_tag="VFU15_11375"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007655251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEU4718430.1"
                     /translation="MTTALIGPPLVAYLVGLPLVFALLMLFAKGEGVKRVAFIASLVE
                     LGLALFAYYTLHKNSLSPQLSMDVPWITSLGVDFNVAIDGISMVLVLLTTILVPIIIL
                     SSFRNNYENPSTFYALIMFMQMALIGVFVARDGFLFYIFWELALIPIYFICLMWGGEN
                     RGKITFKFFVYTLTGSLFMLAALIYLYLNTSIGDTNIHTFDLKALYLAGQKLLPTEQT
                     WVFWGLFLGFAIKMPIFPFHTWQPDTYVTAPTQGTMLLSGIMLKMGTYGIIRWLLPMT
                     PAAWASWGTTALLLCVISIIYASCMALVQKDFKRLIAYSSIAHVGLIAAGVFAFSPDK
                     NNVEGITGAVVQMFSHGINVVGLFFIADILLSRTGTRELDKLGGIRNVSPQFTTLFMI
                     IMLGSVALPLTNGFVGEFLLINGVYQFSATMALFAGLTIILGAVYMLRSFQAIMLGNT
                     KTITASFGELSGNEKTVLLVVCAAVIILGVYPAPLISIIQNDVHQLVSGIMPMHS"
     gene            7862..9289
                     /locus_tag="VFU15_11380"
     CDS             7862..9289
                     /locus_tag="VFU15_11380"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HEU4718431.1"
                     /translation="MKVILALTGLGVLAMLAELFRMRKIVYPLMLAGLAACIGIAVSY
                     WWLDDGPWSRLFSSMILFEGSSVVFSVIILTTAFLWFIMSGDFFKGENSRTDHAVLVI
                     FSLVGAIILTSFSNMTMLFLGVEILSIPVYVLAGANKSSLASNEASMKYFLMGSFASA
                     FLLFGIALVYGGTGSFDLRDIGLAINSAPGGPAAFVYGGIVMMIVGLLFKLSAVPFHF
                     WTPDVYEGAPTQVTAYMSTVVKTAAIVTILRLFLTIVFAGVDTFWIPILAVCTGLTFF
                     IGNITAAVQENVKRMLAYSSISHAGFLLLAVVAFSSFSAKAIIYYTAAYSIASIAAFT
                     VLINVASQSGKDGVDAFNGLGKKNPLLAMAMTIALLSLGGIPPTAGFFGKYYVLNAAL
                     NSGHVWLTVIGILASLVGVYYYFRIIIAMYFKEAEGDHVIAANNVHQALLFITAVITL
                     VLGLLPDLILNIGTIAASGPVAVTP"
     gene            9298..10581
                     /locus_tag="VFU15_11385"
     CDS             9298..10581
                     /locus_tag="VFU15_11385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HEU4718432.1"
                     /translation="MLRKKPGLSTWIFIALVLGIIAGAIAHAHIWGNLDPERLPRFEQ
                     EALEKFATRQVTGFSLLSSIFLNLVKVIVAPLVFSLLLVGLVKTGNFSAMGRIGGKTI
                     LYFTCATLIALTMGLVIVNLFHPGTSLNIHSATEKIVGAQPFNVEEFILHIFPKNIID
                     SLSKNDILPIIVFVLFFAGAISAVGEKGKIVVDFFDAVAHIMLKVTSYVMFFAPLAVF
                     GAVAAVIAVHGLGILFGYAKLIACFFGGLAVFLFAVLPAVCAAFRINYFHLLRLIREP
                     MLIAFGTSSSEAAMPKTINALERFGCPDRIIGFVLPLGYSFNLDGSIMYMTFATVSIA
                     QAYHMDLSIGQQITMMLLLLVTSKGLAGVPRASLVVIAGMLDAFHIPAEGLSIIIAVD
                     WLLDMGRSATNVAGNAVATAVVSKWEKSLGKPETD"
     gene            10645..11280
                     /locus_tag="VFU15_11390"
     CDS             10645..11280
                     /locus_tag="VFU15_11390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008507581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEU4718433.1"
                     /translation="MDFWHFLKQLTDPASILHYGGVALLLAVIFAETGLLIGFFLPGD
                     SLVFLSGMFCSTQPELMGVNISVLLPMMIIAAFLGNLAAYWFGKKTGTALFSREDSFF
                     FKKRYLETTRDFYSRHGGKTLVIGRFLPVIRTFAPILAGVIGVDLKKFLLYNISGAAA
                     WITSVGLGGYFLGRIKWVEENVGYIVIALIIVTLIPLFWASVRRARARRKT"
     gene            11572..12372
                     /locus_tag="VFU15_11395"
     CDS             11572..12372
                     /locus_tag="VFU15_11395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEU4718434.1"
                     /translation="MSNKKTSNVQAYFTVAIIIIEYIISYLVWSKVMGNPKNFENLDP
                     IKGHPINVFGTIYKGGFIVPILMTINLLVITFTIERLFTIMRARGTGNVSNFVRNIRQ
                     LVESEAIDEAMAACDRQKGSIGNVVRSGLEKYRLVANDGSMDKEQKIAAIQKDLEEST
                     SLELPMLSKNLVILSTCASIATLVGLIGTVLGMIRSFAALANSGAPDANALATGISEA
                     LINTAFGICGSAIAIIVYNIFNSQIDRITYGIDEANFSILQTFAAKKH"
     gene            12409..13089
                     /locus_tag="VFU15_11400"
     CDS             12409..13089
                     /locus_tag="VFU15_11400"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEU4718435.1"
                     /translation="MPKIKPPSHSPSLDMTPMVDLAFLLVTFFMLTATMRSPEPVIVD
                     TPSSISDIILPKNTLLITIDTAGRVFFNIEGKQVRVEMLRRMMTKFPDVKFTDEQVAK
                     FGSMQSFGVPIQDLPAYIDADEPTKAKMNAVTKGIPIDTTDNKDQLSYWINFGRVSEY
                     RFRDSLKNAGVKTEELRVAIKADGKAGYKQVDKVISLFKKQDVYRFNLITALEGDPNT
                     PAAGDAKK"
     gene            13096..13677
                     /locus_tag="VFU15_11405"
     CDS             13096..13677
                     /locus_tag="VFU15_11405"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEU4718436.1"
                     /translation="MAEIIRQSTSRGKRRRALPGVRVDMTPLVDLGFLLLTFFVLTSE
                     LAKENAITASFPASEKTQPVNGMTVFVGKNPEKIFWYSGKCDPSVHLNVTGTKNNGLL
                     NVLKNANAVVFRQVEVIDRQHNLGLLNDESWKTKRAALLSDDAVPFVVVKWSEDASYQ
                     DVVNVLDDLNRVHNGKYAVVAETAEDNALINKQ"
     gene            13715..14404
                     /locus_tag="VFU15_11410"
     CDS             13715..14404
                     /locus_tag="VFU15_11410"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEU4718437.1"
                     /translation="MAEVNTDSGGDHGKHGKKGRKKAGNPRVDMTPMVDLAFLLLTFF
                     VLTSNLNKSKTMEINMPKDTKDTAQTTKINYKRAVTILLDGNKEGTIYYYEGKLTENT
                     SLFETTLDPKKGLRSYVMGRNSQIVGDMKDLRNEFKKGNMNDSTFKARKSAIQTKYSD
                     SAPFFIIKWGGDATYGDVISTIDELKISDVEMYALTPISRTELEGLSKKTGRHYKELD
                     TPDPTTAPSPQ"
     gene            14431..15228
                     /locus_tag="VFU15_11415"
     CDS             14431..15228
                     /locus_tag="VFU15_11415"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEU4718438.1"
                     /translation="MANWNNEIVSERNEMVFESRNRSYGAYQIRRQYGRVVLISGGLA
                     TVLLMFFVFLFIINRDAPEKKQVKLKQTEVVLKDPPPIDKNAPPPPPPPPPPPPKIQM
                     QKFTTPEVTEEEVKNPPRQDSLISKIGNTDEQGNDDDGPPADLTPRKDSSIDNNKPEE
                     FFTFAEVMPTYPGPEGAFQSYLQKNIHYPEMEKEQNKQGTVYVSFVVEKDGSITNVAT
                     VKGVDGAPGLSREAERVIKLMPKWTPGQMNGRSVRVQVTQPVRFTLQ"
     gene            15272..15472
                     /locus_tag="VFU15_11420"
     CDS             15272..15472
                     /locus_tag="VFU15_11420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718439.1"
                     /translation="MLLLSMTMVLVFLGLGFLFLFTRVADDRFPAPQRYGIGGVLVAW
                     AAFRTFTVWRKFKDSQKEEENE"
     gene            15465..16403
                     /locus_tag="VFU15_11425"
     CDS             15465..16403
                     /locus_tag="VFU15_11425"
                     /inference="COORDINATES: protein motif:HMM:NF024257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEU4718440.1"
                     /translation="MNSLRRTLVFIPVLFLLVQCETSSRPPEQELDTPTSGKVRFSID
                     ENVAPLADELIDAFEYSYPDAFLVQSYGNEESVVNELFNDSSRLAFMTRKLNKEELSF
                     FESKKFGIEQIPIGKDAVVFIVNRENPDSVFAVDEIRKMLTGEDSLWNEVDSRSSREK
                     ITVVFDNSASSNLRYLTDTLGTGKNLGKNCFAVSTNDSVIAYVQRTPGAVGVIGLNWL
                     GDKDAGSDRERRSKICIAAIGPDRSSAVHPTQSDLVTGAYPFARGIWLVKIGKRADLG
                     TGFASFAYSDRGQLIVQTAGLAPAAPAERKVEINLR"
     gene            16429..>17102
                     /locus_tag="VFU15_11430"
     CDS             16429..>17102
                     /locus_tag="VFU15_11430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718441.1"
                     /translation="MKTMIKNASVAICMLCATGAFAQTLQDAIRYTESEQYEKAKAVL
                     KKLVAADPNNGDNYFYFGDLMLKEESPDSAFILFKKGTDINPTNPLTHVGLGRYYMYQ
                     GKNTEGQQELTNAKSLVQATAGKKDMGMTPKRQALIDLEIAETYIWAPTPDIQQALDL
                     IATAEKLDKDNPEVYLQRGDALYKKDPVNGSPANAAYIIAAQKDPKSCKAYLRMGDII
                     ANGQNMV"
BASE COUNT         3970 a         4750 c         4267 g         4115 t
ORIGIN      
        1 ccgcgcatca agcccccgtt ccctgccgtg cagggcgtgt gggcgcgacc gaccgttgtc
       61 aacaacgtgg aaacgattgc ggccactgtt cccatcgtga acatgggcgg cgaagaatac
      121 gcgaagatcg gcatcggcaa atcaaccgga acgaagctca tttccgcctg cggccacatc
      181 aacaaacccg gtgtttacga gatcgatctc ggccttcccg tcgaagagtt tctttattct
      241 gaaaattact gcggcgggat ccgcaacggt aaaaagctca aggcggttgt cgccggcggc
      301 tcttccgttc ccattcttcc tgcagagctt ttcctcaaaa ccgccaaagg cgaacctcgt
      361 ctcatgtcgt atgaatcgct cgccgacggc gggttccaga caggaacaat gctcggctcc
      421 ggcggattca tcgtgatgga tgaaacaaca agcgtggtga agaacctctg gaacttcacc
      481 cgtttttacc accatgaaag ctgcggacaa tgttcgccct gccgcgaagg aacgggatgg
      541 atggaaaaaa tcctccaccg cattctcgac ggtcacgggc atcccggcga cgtagatctg
      601 ctcttcgaca ttcaatcgaa gatcgacgga aaaacgatct gcccgctcgg tgacgctgct
      661 gcatggcccg tggccagcgc gatccgtcac ttccgcgcgg aatttgagga atacatcaaa
      721 aacccgtctt ccatcaaaga ccagaaacac tattacaacc tgcgcaacca agccgcgccg
      781 gtagcttaac caattagtaa tcgcgattac taaactgaaa acgaatgccg aaagtaacca
      841 tagacggaca cgagatcgaa gtaccgaaag gaacgacgat actgcaggcc gcacgcatga
      901 tcggtaagca tgtggcgcct cctgccatgt gctattactc caagctgaaa acttcgggcg
      961 gctattgccg cacctgcatc gtgcgcgtga cgaaaatgaa ttcgcagaac ccgaacccga
     1021 tgccgaaacc ggtggcttcg tgccggacgg aagtgatgga cggaatggaa gtggtgaact
     1081 tcacctctcc cgaagtgctc gaagcgcggc gcggtgtggt ggaattcctg ctgctgaatc
     1141 acccgctcga ttgccccatc tgcgaccagg ccggcgaatg ccacctgcag gatctcggct
     1201 acgagaacgg tgcggcaaaa acgcgttacg atttcgagcg ccgtacgttc gaaaagatcg
     1261 acatcggtcc ttatgtgcag ctgcacatga cgcgctgcat cctctgctac cgctgcgtga
     1321 aagtggccga acagctcacg gatcaccgcg ttcacggcgt gctcaaccgc ggcgacgtgg
     1381 cggaaatttc gacgttcgtt aaaaattcca tcgacaatga attttccggc aacgtgatcg
     1441 acgtttgtcc cgtcggcgcg ctcaccgaca aaacattccg cttcaaaagc cgcgtatggt
     1501 tcgtgaagcc ggttgacgcc caccgcaatt gcacaaaatg ttccggcaag gtccgtctct
     1561 ggttcaaggg agcggatgtc ctccgcgtca ccgcacgcaa agaccagtgg ggcgaagtgg
     1621 aagactggat ctgcaacgat tgccgcttca accacaaaaa agtttccgac tggaacatcg
     1681 aagggccgag ccacattgat caccattccg tcatttccgc caaccattat gagctcgtga
     1741 agctcaagct cgatacgggc aagcagcttg gaaagctcaa ggggaaaaaa cacaaagaat
     1801 cttccagcca ctaacctatg accacagcgt tcatcattta taagatcatt ctcgttctgc
     1861 tcgtcttcgc ggtttcgctg ggcattgcag cctattcgac atacgcggaa cgaaagatcg
     1921 ccgcattcct gcaggaccgc ctcgggccga accgtgcggg accattcggg atcctccagc
     1981 cgatcgccga cggcatcaag atgttcatga aagaagagat cattccgaac gtttcgaaca
     2041 aatttctttt tgtgctcgga cctgccatcg gaatgttaac cgcgcttatg accggcgccg
     2101 ttattccctg gggcccgaac attatgtggc acggagaatc gcttccgctg caggtaacgg
     2161 acgtgaacat cggcgtgctg tacctgctgg gcgttgtttc gatcggcgtg tacggcatca
     2221 tgatcggcgg atgggcgtcg aacaacaagt attccctgct cggcgcgctg cgcgcttctt
     2281 cgcagatgat cagctatgaa gtggcgatgg gaattgccat cgttacgctc atcatgatga
     2341 cgggaacgct gcagacatcg gcgatcgctg cggagcagca cagcttctgg aacggccatt
     2401 atttttcctg gaactttttc aagaacccgc tcacgttcct catctttttc atctgcgcac
     2461 tggccgaaac gaaccgtaca ccgttcgatc tcccggaatg cgaaacagag cttgtcggcg
     2521 gttaccacac ggaatacagc tcgatgaagc tcggttttta tcttttcgcg gaatacgtga
     2581 acatgtttat ctcctccgcg gtgatgtcgc ttttcttttt cggcggttac aattttccgt
     2641 tcatggacaa gatttccgat ccgaatattg ttgcgctcat cggcacgctg gtcatgtttg
     2701 cgaagacgct gtttttcgtc tttgttttca tgtggatccg ctggacaatc ccgcgtttcc
     2761 gctacgacca gctcatgaat ttaggctgga aaattctgat acctttgtcc ctcctcaacc
     2821 tcatcggcac aggcctggga atgtggggaa tgggcaaact ctgatgcagc tgttttacga
     2881 aagagtttaa ccaaaaaagt gacatcatgc agtcgctgac taacagatca aaagttgtgg
     2941 tgaagaaaga aatgtctttc accgaaaaac tttacctgcc cgctgtgctg aacggaatgc
     3001 gtatcacgct atcccatctt ttcaaaaaga aagcgacgat cagctatccc gaaaaggaac
     3061 gtccgcgcag cagcgtttac cgcggcgaac atgtgctgaa gcgtgatgag aacggggcgg
     3121 aacgctgcac ggcctgcggg ctctgcgccg tcgcatgtcc ggcggaagcg atcacgatgg
     3181 tggccgccga acgcaaaaag ggagaagaga atctttaccg cgaagaaaaa tacgctgctg
     3241 tttacgagat caatatgctg cgctgcattt tctgcgggct ctgtgaagaa gcctgtccga
     3301 aagaagcgat cttcctcacc gaccgcatcg ttcccacgga attcgaacgc cgtgatttcg
     3361 tttacgggaa agacaagctc gtggaacccg tggacaagcg cgtggatgtg acaaagcgcc
     3421 agaccgccgc cgttctcgaa ttcaaaaaac acccggagtt cgctcacaac aaattgtggg
     3481 atccttcccg ttcactcaaa accaaaaagg cccactaacc gaattcagcc attatgaacc
     3541 tgacaattac cgaggtcctt ttcttcgttc tttccgccat agcgctcatc agcggcgcgt
     3601 gcgtcatctt cagccggaat ccggtttaca gtgtcctcta cctcatcgtc acttttttcg
     3661 cgatcgcagg acattacatc ctgctcaatg cacagttcct cgcggttgtg cacatcatcg
     3721 tgtatgccgg cgccatcatg gtgcttttcc ttttcgtgat catgatgctg aacctgaaca
     3781 aggaaacgga gccgcataaa ccggcctgga taaagatcgc cgcagtggtc gcgggcggaa
     3841 cgctgatgct gacgctcgtg ggtgcgctga aaggtgcgga tacgatcgcg ctcaaacaac
     3901 ccaatgatac gcagatcggt cttgtcacac ggctcggtca cgtgctcttc accgatttca
     3961 ttgttccctt tgaaatttct tccgtgcttt tcctcgctgc aatggtcggc gcggtgatgc
     4021 tcggcaaaaa aaatattacg tgatatgtca acaatgctgc tcgatgccgg tcattcggcc
     4081 atccaggtga ttcccatcag ctggtacgtt acgttcagcg ccgttctttt cggaatgggc
     4141 gtgctcggtg tattgtaccg ccgcaatgcg atcatcattc tcatgtgcat cgaaatgatg
     4201 ctgaacgcgg tcaacctgct gctcgttgct ttttctgcac agcacagcga tccgcgcgca
     4261 caggtcttcg tgttcttcgt catggctgtg gccgccgcgg aagttgccgt gggtcttgcc
     4321 attctcatga tcatttaccg caacaccaag actaccgaca tcgacgcatt aaacaagcta
     4381 aaaggataat ttcattatga ttcagctcgc ctggttgatt cctgttctgc ctctccttgg
     4441 ttttgcgatc atcggattgg gcgggaaaaa actttcgaaa ggacagataa gcctcgtcgg
     4501 ctgcggctct gtcctgcttg cattcctgct ttcgctcgga atatttttcc agctgcatgg
     4561 ccaatcggaa gtcgttaaag tggcaagctg gtttgccacg ggccgccttt ctgtcgattt
     4621 ctctttcctg atcgatccgc tttcggtgct tttcatgctc atcattacgg gcgtcgggtt
     4681 tttgatccac ctgtattcca cgggctacat gcacgacgac gacggctacc accgcttctt
     4741 ctcgtacatg aacctgttcg ttttcttcat gctgctcctc gttatgggaa gcaactacat
     4801 cctcatgttc gtgggatggg aaggcgtcgg tctctgttcc tacctgctca tcgggttctg
     4861 gttcaaaaac acggaataca acaacgcggc gaaaaaagca ttcatcgtca accgcatcgg
     4921 cgatctcggg tttttgctgg gaatgatcct catttacgtg acgttcaaaa gcctcgactt
     4981 cagcgtcgtt ttcaaaggcg tgcacgataa cgcgtacaac cagagcatct acaacaccat
     5041 cggcatcctg ctcttcatcg gcgcctgcgg aaaaagcgcg cagataccgc tgtacacatg
     5101 gctgcccgat gcaatggccg gaccgacacc ggtttccgcg ctcatccacg ccgcaacgat
     5161 ggtcacgtcc ggcatttaca tgatcacgcg ctcttccgtc attttcatca atgctcctgt
     5221 agccagcgaa gtggttgcgc ttatcggcgt gctcacggcg gtcttcgcgg caacgatcgg
     5281 cctgttccag aacgacatca agaaagtgct cgcctattcc acggtcagcc agctgggcta
     5341 catgttcctc ggtctcggcg tcggtgcatt caccggcggt gttttccacg tgatgacgca
     5401 cgcgttcttc aaagcgctgc tgttcctgtg cgcggggagc gttatccatg ccatgagcgg
     5461 cgaacaggat atccgcagga tgggcggact gaaaaaagca ttacctataa ctcacctcac
     5521 atttctcatc ggaacgcttg ccatttcggg gctgccgcca ttctccggct ttttctcgaa
     5581 agatgaaatt ctcgggcatg tatacgaaca tgaaggttcc gggaaacttt actgggtgct
     5641 cggcgtgatc ggttcgatgc tgaccgcatt ctacatgttc cggctctatt ttctcacttt
     5701 ccacggaaaa ttccgcggta cgcacgaaca ggaacatcac ctgcacgagt cgccgaaaag
     5761 catgaccgtt ccgctgatga ttcttgccgg gctcgcagct gttgcgggat tccttggcat
     5821 tccgaatgcc caccagcacc tgctgtacaa ttttctcgaa ccgggttacg gttacgccaa
     5881 aacctggatc gagggccctt cacattttgg cggcgaagaa ttgaagctca tgctcattgc
     5941 catcgcttct gcaggcgccg cgatctatct cgccttcatg atctacagga agaacaacgt
     6001 tctcccgaaa gaaaaagaag aggacatgcc gaaaatccag cgcgttattt tccacaaata
     6061 ttacgtggat gaattctacg atgccgtgat ccgcaagccg ctcgactggc tgtccgaaat
     6121 gttttacaaa ttcttcgagc tcaaattcat tgatggcatt gtgaacggca taggctcggg
     6181 cgtgaagtgg atgggctcaa tggcgcgtta cgtgcagaca ggcagcatcg ggttctacct
     6241 gttcgcgatg tcccttgctg tgatgctcat ctttatgatc aaattctttt aataccggct
     6301 gaataaacat gacaaccgca cttattggcc ctccgcttgt agcatacctg gtaggtcttc
     6361 cgctcgtatt cgcgctgctg atgcttttcg ccaaaggcga gggcgtgaaa cgtgtcgcgt
     6421 tcatcgcatc gctcgtagaa ctcgggctgg cgcttttcgc ttactacacg ctgcataaga
     6481 attccctcag cccgcagctg tccatggacg ttccgtggat cacttctctc ggcgtggatt
     6541 tcaacgtggc cattgacggc atcagcatgg tgctcgtgct gctgacaacg attcttgttc
     6601 ccatcattat tctcagcagc ttccgcaaca attacgaaaa cccttccaca ttttatgcgc
     6661 tgatcatgtt catgcagatg gcgctcatcg gcgtcttcgt ggcgcgtgac ggatttctct
     6721 tctacatttt ctgggagctc gcgctgatcc cgatctattt catctgcctg atgtggggcg
     6781 gggaaaaccg cggaaaaatc acgttcaagt ttttcgtgta cacacttacc gggagccttt
     6841 tcatgctcgc ggcgctcatc tatttgtacc tcaatacgtc gatcggcgac accaacatcc
     6901 acacgttcga cctgaaggct ctttatctcg ccggacaaaa gctgctgccg accgaacaga
     6961 cctgggtttt ctggggactc ttcctcggct tcgcgatcaa gatgccgatc ttccccttcc
     7021 acacctggca gcctgacacg tatgtcaccg cacccacgca gggcacgatg cttctttccg
     7081 gcatcatgct gaaaatgggc acgtacggga tcatccgctg gctgctgccc atgacacccg
     7141 ctgcgtgggc gagctgggga accacggccc tgctgctttg cgtcatcagc atcatctacg
     7201 cgtcctgcat ggcgctggta cagaaagatt tcaagcggct catcgcctat tcttccattg
     7261 cgcacgtcgg gctgatcgct gcgggtgttt tcgcattttc tcctgataaa aacaacgtcg
     7321 aagggatcac gggtgctgtg gtgcagatgt tcagccacgg cataaacgtc gtcgggctgt
     7381 tcttcatcgc cgacattctt ctttcacgca caggcacacg tgagctcgac aagctcggcg
     7441 gtatccgcaa cgtttccccg caattcacca cgctcttcat gatcatcatg ctcggctcgg
     7501 tggcgcttcc gcttacaaat ggcttcgtag gcgaattcct gctgatcaac ggcgtttacc
     7561 agttcagcgc gacgatggcg ctgttcgccg ggctcacgat cattctcggc gcggtgtaca
     7621 tgctgaggag cttccaggca atcatgctcg gtaatacgaa aacaataaca gcttccttcg
     7681 gcgagctgag cggcaatgaa aaaaccgttc tcctcgtcgt atgtgcggcg gtgatcattc
     7741 tcggtgttta tcccgcaccg ctgatcagca taatacagaa cgatgttcac cagctggtga
     7801 gcgggatcat gcccatgcat tcctgattaa taatcacggt tattattcca gaaaataaaa
     7861 tatgaaagtc attttagcac ttaccggtct tggcgtcctt gcaatgctcg ctgaactttt
     7921 caggatgcgg aagatcgttt acccgctgat gctggccggt ctcgccgcct gcatcggcat
     7981 cgctgtttct tactggtggt tagacgacgg cccctggagc cgcctgttca gcagcatgat
     8041 cctcttcgaa ggttcttccg ttgttttttc cgtgatcatc ctgacaacgg ctttcctgtg
     8101 gttcatcatg tccggcgatt ttttcaaggg cgagaacagc cggacggacc atgcggtgct
     8161 tgtcattttc tccctcgtcg gcgcgatcat cctcacctct ttcagcaaca tgacgatgct
     8221 ttttctcggc gttgaaattc tttccattcc cgtttatgtg ctcgccggtg caaacaaaag
     8281 cagtctcgct tcgaatgaag cgtccatgaa atatttcctg atgggctctt tcgcttccgc
     8341 attcctgctt ttcggaattg cgctcgtata cggaggaacg ggttcatttg acctgcgcga
     8401 tatcggtctt gcgatcaaca gtgcgcccgg cggacccgct gcatttgtat acggcggcat
     8461 cgtgatgatg atcgttggac tgctcttcaa gctttcggca gtgcctttcc atttctggac
     8521 gcccgacgtt tacgagggtg cgccaacaca ggtaacagct tacatgtcga ccgtggtgaa
     8581 aacagccgcg atcgtgacga tcctgcgtct cttcctgacg atcgttttcg ccggcgtgga
     8641 tacgttctgg atcccgatcc tcgctgtttg cacgggactt acgttcttca tcgggaacat
     8701 cacggcggcc gtgcaggaaa atgtaaaacg catgcttgcc tattcgagca tttcacacgc
     8761 cggcttcctg ctgctggccg tggtggcgtt cagctctttc tccgccaagg ccatcattta
     8821 ttacaccgcg gcgtattcca tcgcatctat cgccgcgttc acggtattga tcaacgttgc
     8881 ctcgcaatcg ggaaaagacg gcgtcgatgc gtttaacggg ctcgggaaga aaaatccgct
     8941 gttggccatg gcgatgacga tcgcgcttct ttcgcttggc gggattcctc ccacagccgg
     9001 gtttttcgga aaatactacg tgctgaatgc tgccctgaac tccggccacg tctggctcac
     9061 ggtgatcggc atcctcgctt cccttgtcgg cgtgtactat tatttccgca tcatcatcgc
     9121 gatgtatttc aaagaagcgg aaggcgatca cgtcattgcc gccaataacg ttcaccaggc
     9181 gctgcttttt ataaccgcgg ttattacttt ggtgctgggc ctgcttcccg acctcattct
     9241 caatatcggc acgatcgccg cttcgggccc ggtcgccgta acgccctgaa cggataaatg
     9301 ctgcgcaaaa aaccgggtct ctccacgtgg attttcatcg ccctggtcct gggaataatt
     9361 gccggcgcaa tcgctcacgc gcacatctgg ggaaatctcg atcctgaacg gcttccccgt
     9421 ttcgaacagg aggcgctcga aaaatttgca acgcggcagg tgaccggctt cagcctgctc
     9481 agcagcattt tcctgaacct tgtcaaagtc atcgttgcgc cgctggtgtt ttcgctgctg
     9541 ctcgtcgggc tcgtgaaaac cggcaacttt tccgcaatgg ggcgcatcgg cgggaaaacg
     9601 attctctatt tcacgtgcgc cacgctgatc gcccttacga tgggactcgt catcgtgaat
     9661 ctttttcatc ccggcacttc gctgaatatc cactccgcaa cggagaagat cgtcggggcg
     9721 caaccgttca atgtggagga gttcatcctt cacatcttcc cgaaaaacat catcgattcg
     9781 ttatcgaaaa acgacatcct tcccatcatc gttttcgtcc tgttctttgc cggcgcgatt
     9841 tccgctgtcg gtgaaaaagg aaagatcgtg gtcgatttct ttgacgccgt ggcgcacatc
     9901 atgctgaagg ttaccagcta tgtaatgttt ttcgctcccc tggccgtttt cggagccgtt
     9961 gctgcagtga tcgccgttca cgggctcggc attcttttcg gctatgcaaa gctgatcgcc
    10021 tgctttttcg ggggacttgc cgtttttctt tttgccgttc ttcctgcggt ctgcgcggcg
    10081 ttccgcataa attatttcca cctgctgcgt cttatccgcg agccgatgct catcgctttc
    10141 ggcacctcca gctcggaagc cgccatgccg aaaacgataa atgcgctcga acgcttcggc
    10201 tgtcccgacc gcatcatcgg cttcgttctt ccgctcggtt actcgttcaa cctcgacggg
    10261 tccatcatgt acatgacgtt cgccacggtt tccattgcgc aggcctacca catggatctt
    10321 tccatcggcc agcagataac catgatgctg ctgttgctgg tgacgagcaa aggcctggcc
    10381 ggcgttccgc gcgcctcgct tgtggtcatt gcgggaatgc tggatgcatt tcatattcct
    10441 gcggaaggac tttccatcat cattgctgtc gactggctgc tcgacatggg ccgctccgcc
    10501 acgaatgtcg ccggaaacgc ggtggccaca gcggtagtga gcaaatggga aaaatcgctg
    10561 ggcaaaccgg aaaccgactg acatttattt tccttccgcg gaatattccg cctgtaaaaa
    10621 cgggaacttt acatttttcc cggtatggat ttctggcatt tccttaaaca gctcaccgat
    10681 cccgcgtcga tcctgcatta tggcggagtt gcgctgctgc ttgctgttat ttttgccgaa
    10741 accgggctgc tcatcgggtt ttttcttccc ggcgattcgc tcgttttcct ctcgggaatg
    10801 ttctgctcca cgcagccgga gctgatgggc gtgaatattt ccgtgctgct gccgatgatg
    10861 atcatcgccg cttttctcgg caaccttgcg gcttactggt tcgggaaaaa aacaggtacg
    10921 gcacttttct cgagagaaga ttcctttttc ttcaaaaaac gctacctcga gaccacccgc
    10981 gatttttatt cccgtcacgg cggaaaaacg ctggtgatcg gccggtttct tcccgtcatc
    11041 cgcacctttg cgcccattct tgcaggcgtc atcggcgtgg atctcaaaaa attcctgctc
    11101 tacaatattt cgggcgcagc agcatggatc acctcggtgg gactaggcgg ctatttcctg
    11161 gggcgcataa aatgggtgga agaaaatgtc ggctacatcg tcattgcgct tatcattgtc
    11221 acgctgatac cccttttctg ggcctcggtc cgccgcgcac gggcccgcag gaagacctga
    11281 aagcctcctt ttttaccgat tatttctgcc cggcgtccac ttatgaaacg gtttccccac
    11341 gggtataatt aatgcgttat tttgcattag ttatcatgaa aaacaggaat gaaaaaccgt
    11401 gtttttgcaa tttcgggaat aatccgcttt tttgtatgtt tgaacccttt cgaaggccgg
    11461 agattgccga aattaacagg gagttaaaaa gcctgcgaaa tgatttcgct atcattgcgt
    11521 ccccggaaca gtaattaatc taaccaatac aaaaccaaac cttttcttac tatgagcaac
    11581 aagaagacct ctaacgtgca ggcctatttt acggttgcaa tcatcattat cgagtacatc
    11641 atttcgtatc tcgtatggtc aaaagtgatg ggcaacccta agaacttcga aaatctcgac
    11701 ccgatcaaag gtcacccgat taacgttttc ggcaccatct ataaaggcgg attcatcgtg
    11761 cctatcctga tgacgatcaa cctccttgtt atcacattca ccattgagcg tcttttcacc
    11821 atcatgcgcg cccgcggaac cggcaacgtt tccaacttcg tccgcaacat ccgccagctc
    11881 gtggaaagcg aagcgatcga cgaagcaatg gcagcatgcg accgccagaa aggttctatc
    11941 ggaaatgtgg tccgctcggg ccttgaaaaa taccgcctcg tggcaaacga cggctccatg
    12001 gataaagagc agaagatcgc cgcgatccag aaagatctcg aagagtccac ctcgcttgag
    12061 cttcccatgc tctccaaaaa ccttgttatt ctttccacct gcgcgtccat cgcaacgctg
    12121 gtcggtctga tcggaaccgt actcgggatg atccgttcct tcgccgctct tgcgaattcc
    12181 ggcgcgcctg atgcgaacgc acttgcaacc ggtatttccg aagcgcttat caataccgcc
    12241 ttcggtatct gcggttctgc catcgccatc atcgtgtaca acattttcaa ttcgcagatc
    12301 gaccgtatca cctacggcat tgacgaagcg aacttcagca tcctgcaaac cttcgctgct
    12361 aaaaaacact aagtattttc tcagccaagg attttaaaaa cacagcaaat gccaaaaatc
    12421 aagccaccga gccacagccc ttccctggac atgacgccca tggtggatct tgcgttcctg
    12481 ctggtgactt tcttcatgct tacggcaacc atgcgctctc cggagcccgt gatcgttgat
    12541 acgccaagct ccatttcgga tatcatcctt ccgaaaaaca cgctgctgat cacgatcgat
    12601 acggccggac gcgttttctt caacatcgaa ggaaaacagg tccgtgttga aatgctgcgc
    12661 cgcatgatga ccaagttccc tgacgtaaaa ttcactgacg agcaggttgc caagttcggc
    12721 tccatgcaat ctttcggcgt gccgatccag gatcttcccg cctacatcga tgcggatgaa
    12781 cccacaaagg ccaaaatgaa tgcggtcacc aaggggattc ccatcgacac aacggacaac
    12841 aaagaccagc tttcctactg gatcaacttc ggccgtgttt ccgaataccg tttccgcgat
    12901 tcgctcaaaa atgccggcgt gaaaaccgaa gagcttcgcg ttgccatcaa ggctgacggt
    12961 aaggcgggct acaagcaggt ggacaaagtg atctccctgt tcaaaaagca ggacgtgtat
    13021 cgcttcaacc tgatcaccgc gcttgaaggc gatcccaata cccctgcagc aggagacgca
    13081 aaaaaataac gcaaaatggc agagatcatc cggcaaagca catcgagggg aaaacgccgc
    13141 agggcgctcc cgggcgtgcg cgtcgacatg acgccgctgg tggacctcgg cttcctgctg
    13201 cttacttttt ttgtgcttac ctctgagctg gccaaagaaa atgcgatcac ggcgtcattt
    13261 cccgcaagcg aaaaaacgca gccggtgaac ggcatgacgg ttttcgttgg aaaaaacccg
    13321 gaaaaaatct tctggtatag tgggaaatgt gacccgtccg tgcatcttaa tgttacaggc
    13381 acaaagaaca acggcctgct caacgttctt aaaaatgcga atgctgttgt tttccgccag
    13441 gttgaggtga tcgaccggca gcacaacctc ggtcttctga atgacgagtc ctggaaaacc
    13501 aaaagggctg cgcttctttc ggatgacgct gttcccttcg tagttgtaaa atggagcgaa
    13561 gacgcgtcgt accaggatgt ggtgaatgtg ctggatgacc tcaaccgcgt gcacaacgga
    13621 aaatatgcgg ttgttgcaga gaccgcggaa gacaatgcac tgatcaacaa acaataagag
    13681 aaggattttt tccttcatca aaacgaaata aaaaatggca gaagtaaata cagacagtgg
    13741 cggtgaccat ggcaaacacg ggaaaaaagg ccgcaaaaag gccggcaatc cgcgcgtgga
    13801 catgacgccc atggtcgacc tcgcgtttct tcttctcacg ttcttcgtcc tcacgtccaa
    13861 cctgaacaag tcgaagacga tggagatcaa catgccgaaa gacaccaagg acacggccca
    13921 gaccaccaag atcaactaca agcgggctgt taccatactt ctcgacggca acaaggaagg
    13981 aacgatctat tactacgaag gaaaacttac cgaaaacact tcgcttttcg aaacaaccct
    14041 ggacccgaag aaaggtctcc gttcttatgt aatggggcgc aacagccaga tcgtcggcga
    14101 tatgaaggat ctgcgaaatg agttcaagaa aggaaacatg aacgacagca ctttcaaggc
    14161 aagaaaatca gcgatacaaa ccaagtattc cgactctgcc ccgttcttca tcataaaatg
    14221 gggaggagat gctacgtatg gcgatgttat cagcacgatt gatgagctga aaatatcaga
    14281 cgttgaaatg tatgcgctta cgccgatctc gcgtacagag ctcgaaggat tatcaaaaaa
    14341 gaccgggcgt cattacaaag aactggatac gcccgatccc acaaccgcac catcccctca
    14401 atgaagttaa tctcataaat tcctatccaa atggctaact ggaataatga aattgtaagc
    14461 gaacgcaatg agatggtttt cgaaagccgg aaccgttcct atggcgctta ccagatacga
    14521 agacagtacg gtcgggttgt cctgatctcc ggggggcttg ctactgttct gctcatgttc
    14581 ttcgtgttcc tgtttatcat caacagggac gctcccgaaa aaaaacaggt gaagctcaag
    14641 cagacggaag tcgtgctcaa agatccgccg ccgatcgaca agaacgcgcc gccaccacca
    14701 ccgccgccgc cgccgccgcc gccgaaaatc cagatgcaga aattcacgac cccggaggtt
    14761 accgaagagg aagtgaaaaa tccgccgcgc caggacagcc tgatttccaa gatcggcaat
    14821 acggatgagc agggtaatga tgacgacggc ccgccggccg atcttacgcc gcgcaaagat
    14881 tcctccatcg acaacaataa acctgaagag ttcttcacgt tcgccgaagt aatgccgacc
    14941 tatcccggac ctgaaggcgc attccagagc tatctccaga agaatattca ttatcctgaa
    15001 atggagaagg aacagaacaa gcagggaact gtttatgttt cgttcgtcgt ggaaaaagac
    15061 ggttcgatca cgaacgtcgc aacggtaaaa ggtgttgatg gcgctccggg gctcagccgc
    15121 gaagcggaac gcgtgatcaa gctcatgccg aaatggacac cgggccagat gaacggccgc
    15181 tccgttcgtg tgcaggttac gcagccggtg cgttttacgc tgcaataatt ttttcgcgtt
    15241 gcgactggaa aacccgggtc ccggaaaaat aatgctcctt ctgtcgatga caatggtgct
    15301 tgttttcctt ggtctgggtt ttttatttct cttcacccgc gtcgcagacg accggtttcc
    15361 cgctccgcaa cgttacggca taggaggcgt tctcgttgca tgggctgcct tccggacgtt
    15421 tacggtctgg aggaaattca aggacagtca aaaagaagaa gaaaatgaat agtctccgcc
    15481 gcacacttgt tttcattccg gtcctgttcc tcctggttca gtgcgaaacg tcttcccgcc
    15541 ccccggaaca ggagctggat actcccacaa gcggaaaagt ccgtttttcc attgacgaaa
    15601 atgtggcgcc ccttgcggac gagcttattg atgcgtttga atattcctac cctgacgcat
    15661 ttctcgttca gtcgtacggt aatgaagaat ctgtcgtgaa cgagcttttc aatgattctt
    15721 cccgccttgc gtttatgacg cgaaagctga acaaagaaga gttgtccttt ttcgaatcga
    15781 aaaaattcgg catcgaacag atcccgatcg ggaaagacgc tgttgtattt attgttaacc
    15841 gagaaaatcc cgacagtgtt tttgccgttg atgaaatacg gaaaatgctg accggcgaag
    15901 attctttatg gaacgaggtc gattcccgct cctcccggga gaagatcact gttgttttcg
    15961 ataacagcgc atcttccaac ctgcgttacc tcaccgatac gctcgggaca ggaaaaaatc
    16021 ttggcaaaaa ttgctttgcc gtcagcacga acgattccgt cattgcctat gtgcagcgaa
    16081 ccccgggggc cgtgggcgtg atcggcctta actggcttgg cgacaaagat gcgggaagcg
    16141 accgcgagcg ccgttcgaag atctgcattg ccgccatcgg gcccgaccgt tcttccgctg
    16201 ttcatcccac gcaaagcgat cttgttacgg gcgcatatcc ttttgccagg gggatctggc
    16261 tggtcaaaat cgggaaaagg gccgaccttg gcacaggctt tgcctccttt gcatacagcg
    16321 accggggaca gctgatcgtt cagacagcgg ggcttgctcc ggcagcgccg gcggaaagaa
    16381 aagtagaaat taacctccgc tgaaaaacag atagttattt ttgcaattat gaaaacaatg
    16441 atcaaaaacg cttcggttgc aatttgcatg ctttgtgcaa caggcgcgtt cgcacaaacg
    16501 ctccaggatg ctatccgcta cacggaaagc gaacaatacg aaaaggctaa agcagtactt
    16561 aaaaaactcg tggccgccga tccgaacaac ggggacaatt atttttactt cggcgacctt
    16621 atgctgaaag aggaatcccc cgattccgct tttattcttt tcaaaaaagg caccgacatc
    16681 aatcccacga acccgcttac gcatgtcggg cttggccgct attacatgta ccagggaaaa
    16741 aacacggaag ggcagcagga gcttaccaat gcgaaatcgc tggtgcaggc caccgcagga
    16801 aaaaaggata tgggcatgac gcccaagcgc caggcactta tcgatctcga gatcgcggaa
    16861 acctatatct gggcgcccac gcccgatatt cagcaggcgc tcgatcttat tgccaccgcg
    16921 gaaaagctgg acaaggataa cccggaagtt tacctgcagc gcggggatgc gttgtacaaa
    16981 aaagacccgg tgaacggctc tcccgcaaac gccgcataca tcatcgccgc gcaaaaagat
    17041 ccgaaatcct gcaaggcata cctgcgcatg ggtgatatca tcgccaatgg ccagaacatg
    17101 gt
//
LOCUS       DASTSF010000399         6399 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_28427,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000399 DASTSF010000000
VERSION     DASTSF010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 6399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6399
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28427"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(109..1464)
                     /locus_tag="VFU15_11435"
     CDS             complement(109..1464)
                     /locus_tag="VFU15_11435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015903205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU4718442.1"
                     /translation="MVDNDPILLNSETLEKEIAWISEVIDTRLKLYFGHECTYGDIYE
                     ITPPEPAHRDSMYASLVKHYNLSFHERAVLMLGLIPHIRPQLLDVFFVKNGNVDRGFT
                     EFGGVKGNGHGGFLPTGETALFLLAGENLEQRFALQYLFDGDHFFSMHAILRLEPSQG
                     FDPFLSGVINVSREIIDFVTLGHARRPNFDRDFPARRITTPMDWEDLVLEEQTFVHLD
                     EIKAWIEFEHTIMNEWNMRKKLKPGYRALFYGPPGTGKTLTASLLGKATDREVYRIDL
                     SMVVSKYIGETEKNLSKIFEQAEHKKWLLFFDEADALFGKRTKVEDAHDRYANQEVSY
                     LLQRIEDFEGIVILASNFKSNLDEAFTRRFQSVIHFPMPRPSERLLLWQKAFSDRTVL
                     EPAIRLDEISMQYEISGGAMMNVVRYCSLMALRRGGNTILLSDLEEGIRREYRKEGRT
                     V"
     gene            complement(1458..3179)
                     /locus_tag="VFU15_11440"
     CDS             complement(1458..3179)
                     /locus_tag="VFU15_11440"
                     /inference="COORDINATES: protein motif:HMM:NF039901.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="contractile injection system tape measure
                     protein"
                     /protein_id="HEU4718443.1"
                     /translation="MNKNVINRQIIDLKVSSQKNAFRLQQQVNEIYHAEILPMLDDVM
                     SGLIPENEVLRIDRLEIDLGALRLDGNSTQLTERLRSQISDQLKTQLLKASVHNGVTA
                     STEQDEAPALVKETRSALDLLDYFFSSGSLPWWAGDELQEPDINRMIILLLEKEPVSL
                     LQLLQKTAHHPVSARRLVLQTETRTRSQLLSFFSSSIRILQQQLQVLRKITRNDTAVL
                     VAAGHESEVLLLAVLLSGESAATGMLSKETAATALIRANAFVAGKKAEAIESRLYMHI
                     VNAAAKDEKTEMRETGLLPYFLEWEKKHRGTVIHSSGDPVLPLAKWVKEFTGAPATKT
                     AQEAESISKKKHTRGRKKDSETAPAENKANAVKKNEHTGTEDAVKEKRPMHHSDENVQ
                     VFSSSEEENEFPGGDSPAGMTRYGGLVLIAPFLPAFFSELKLLKENSFTDDAAKYKAV
                     HLLHYIATGKTKAQEYSLMLHKLLCGMKISDPVPKSVRLSRAEKKEAMVFLDDIAEQW
                     TALRTTSGEMIRNTFMRRNGIIEKKDGAWLVRIERGAVDIILNTLPWNISIFKAPWTQ
                     QLLHIEW"
     gene            complement(3194..>6399)
                     /locus_tag="VFU15_11445"
     CDS             complement(3194..>6399)
                     /locus_tag="VFU15_11445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718444.1"
                     /translation="RFLQSCGKSLSPFPKHLTIGRLKNGVTQPYDIYRTVFFNVPPFD
                     AQDHHLNNARMLFRKMLLLLCDFTDPSLRTWKTDKTLITPDGSAMQLLSDRSIPYYYS
                     ESKQKTELLPVWNPEKTLRGRQADRPGYFSGTYSSVPYIISPLNYSILDKPLLRVEGI
                     AGKRVENVRNDLIALKKERNLNFDVLLVKLEADAADPDTSILSLQPDPRESDHCMFDD
                     LQADYLLVRNEIICCVRALQDILCKEMSAPVKYDKIIFEKFSKLFSGKLTLRSFSGLT
                     AAAKKTIDAFGKAAVPSYVTANYSAATFLQLSELVTKMCSLSQPVSKSIVKRLPNLLA
                     DFDYEIFLKEYKQLQHFVIQYRSYFEIVLEINEQFFYDTEMTVSQEIQYSGYRDIINR
                     LRWLDCSCIYPKLGILRYLRDQRIAAKTIAPVFSQYAKEHCGLEHYAGVPQNGTLLLV
                     YSKTNFYDGTINPVVADFALPGICCSPCLTDTKKELQQPLIALDSEITILQDDLLALG
                     TDGLVIDVLSNDYYLARPLLPVADVVLEETDSPDDFKNTGSISIDTSAKRAVVVYKNE
                     KADPTVDFFLLSVRNPETKEMDYCTLRVIVETPAFRLINTHPDKAATLVGRKVNIDLL
                     SNDNSWLDWQENITVTLVKSDGSIPEGDYLSEKGNLVTLDTDGVTAIFDSSNTPGTIS
                     DTVTDRFYYLVTDKSNNRSVVTYVEVDILACCGSIIFQLPRLQYCKNSPPDNFTIDAD
                     GADPSTLYVDGKGASLKNGQWQFDPSSTEVKTGIVPFKLFESNKMKKQLATFQVTVNQ
                     AVDAAFTYTTSFPNDTAFGVANCTVSDRSADLYEWFIEGSLVGEGPTYAAFYYIFASY
                     NLTLVTRKIFGTDNICTSQYTSVIGRQQVDLPQGTFNTLTLFDANNSTVTDLASNKTF
                     SSAAGKSDKTLITDLKKIGTTMQSVLTDTSGTTSLVAGTLDGSIAQSGQVLTRLKDAM
                     ATVTDTKVLTSAQDTSKALAMNTLLAMSMRNPSVAENAPMTDTVGQIGDALTVLADTN
                     AASFTADDRNMLAHAYTLASGQPELQKMIAGVQLKLQ"
BASE COUNT         1336 a         1623 c         1761 g         1679 t
ORIGIN      
        1 cgcttcgtgc gcagcgcccc ggtgggaagg tggcgttaca gtggccgctt ttcccgccga
       61 ttttgttttc gtggatgtgc ctgctgctgt cgacaagctg taatcgtttt aaaccgttcg
      121 gccttctttc ctgtattcac ggcgaatacc ttcttcgaga tcggaaagca ggatcgtatt
      181 tccgccgcgg cgcaacgcca tcagcgagca ataacgtact acgttcatca tcgccccgcc
      241 ggagatctcg tactgcatgg atatttcatc aagacgtatt gccggctcga gcaccgtgcg
      301 gtcggagaac gctttctgcc agagcaacag tcgctctgac ggacgcggca tgggaaaatg
      361 aatgacgctc tggaaacgcc gcgtgaacgc ttcgtcgagg ttgcttttga aattggaagc
      421 gagaatgacg atgccttcga aatcttcgat gcgctgcagg agatagctta cttcctggtt
      481 ggcgtaacgg tcgtgcgcgt cttccacctt cgtccgtttt ccgaaaagcg catcggcttc
      541 atcgaagaag agcagccatt ttttatgttc cgcctgctcg aagatcttcg aaaggttctt
      601 ttccgtttcg ccgatgtatt tcgaaaccac catggacagg tcgatgcggt acacctcgcg
      661 gtccgtggct tttcccagca ggctcgcggt gagcgttttc cctgtgccgg gaggaccata
      721 gaacaatgcg cggtaaccgg gcttcagctt tttccgcatg ttccattcgt tcatgatcgt
      781 atgctcgaat tcgatccatg ctttgatctc atcgaggtga acgaacgtct gctcttcgag
      841 aacgagatct tcccagtcca tcggtgtcgt gatgcgtctt gcgggaaagt cgcggtcgaa
      901 attgggacgg cgtgcgtgac cgagcgtgac aaaatcgatg atctcgcgcg atacgttgat
      961 gacgccgctg aggaaaggat cgaatccctg cgaaggttcg aggcggagaa tcgcatgcat
     1021 ggaaaagaaa tgatcaccgt cgaacaaata ctggagcgca aaacgctgct cgaggttttc
     1081 tcccgccagc aggaaaagcg cagtttcccc ggtcggcagg aaaccgccgt ggccgtttcc
     1141 ttttacgcct ccgaattccg tgaacccgcg atcgacattg ccgttcttca cgaagaaaac
     1201 atccagcagt tgcggccgta tgtgcggaat aagtccgagc atgagcaccg cccgttcgtg
     1261 gaagctcagg ttgtaatgct tcaccagcga agcgtacatg gaatcgcggt gcgcgggttc
     1321 gggcggcgtg atctcgtaaa tgtcgccgta cgtgcattcg tgaccgaaat acagcttcag
     1381 gcgtgtgtcg atcacttccg agatccatgc aatttctttt tccagcgttt ccgaattgag
     1441 aaggatcgga tcgttgtcta ccattcgatg tgcagaagtt gttgcgtcca tggtgctttg
     1501 aagattgata tgttccaggg taacgtgttg agaataatgt ccaccgctcc gcgttcgatg
     1561 cgtacgagcc aggcgccgtc ttttttttca atgatgccgt tccgccgcat gaaggtattc
     1621 cggatcattt cgccggacgt tgtgcgcagc gcggtccact gctccgcgat atcgtcgagg
     1681 aaaaccatgg cttctttttt ttccgcccgg ctcagcctga ccgatttggg aacgggatcg
     1741 cttattttca ttccgcacaa taatttgtgc agcatgagcg aatattcctg cgcttttgtt
     1801 ttaccggtgg cgatgtaatg cagcaggtga acggctttgt attttgccgc atcgtccgta
     1861 aatgaatttt ctttcagaag cttcagctcg gaaaagaaag cgggaaggaa aggcgcgatc
     1921 agcacaagcc cgccgtaacg cgtcattcct gcaggagaat cgccgcccgg aaattcattc
     1981 tcttcttccg aagaagaaaa tacctggacg ttctcatcgc tgtggtgcat cggccgtttt
     2041 tctttcacag catcttccgt tccggtgtgc tcatttttct ttaccgcatt cgctttgttt
     2101 tctgcgggag ctgtttcact atcttttttt cttccgcggg tatgtttttt tttcgaaatg
     2161 ctttcagctt cctgcgcggt ttttgttgcc ggagctccgg taaattcttt cacccatttc
     2221 gccaacggca acaccggatc accgctgcta tgaatgacgg ttccccggtg ctttttttcc
     2281 cattccagga aataggggag aagccccgtt tcccgcatct ccgttttttc gtctttggct
     2341 gcggcgttga cgatgtgcat gtacagccgt gattcaatgg cttctgcttt ctttcccgcg
     2401 acgaacgcgt ttgcacggat caatgccgtt gcggcggttt ctttgctcag catgccggtt
     2461 gctgctgact cgccggaaag caatacggca agcagcagca cttcactttc gtgaccggca
     2521 gcgacaagaa cggcggtatc gttcctggtt attttgcgca ggacctgcag ctgttgctgc
     2581 agtatgcgga tcgaagaaga aaaaaaggaa aggagctgtg agcgtgtgcg cgtttccgtt
     2641 tgcaggacga ggcgccgtgc gctgacagga tgatgcgcgg ttttctgcag cagttgaagc
     2701 aggctgaccg gctctttctc caacaggaga atgatcatcc tgttgatgtc cggctcctgt
     2761 agctcatccc cggcccacca gggcaggctg ccgctggaga aaaaataatc caggaggtcg
     2821 agcgctgaac gtgtttcttt caccagcgca ggagcttcgt cctgttccgt ggatgccgtt
     2881 acaccattgt gaaccgatgc tttcagcaat tgtgttttca gctggtcgct gatctgcgaa
     2941 cgaagccgtt ccgtcagctg tgtgctgttg ccgtcgagac gcagcgcacc gagatcgatc
     3001 tcgagtctgt caatgcggag cacttcgttt tccggaataa gtcccgacat cacatcatca
     3061 agcatcggca gtatctccgc atggtaaatt tcatttacct gctgctgcaa ccggaaagcg
     3121 ttcttctgcg aagaaacttt cagatcgatg atttgcctgt tgatgacatt tttgttcatt
     3181 cccggttatc ctgttattga agtttcagct gcacgccggc gatcattttc tgaagttccg
     3241 gctgacctga agcgagcgta taagcgtgcg ccagcatgtt cctgtcgtcg gcggtgaagg
     3301 aagcagcgtt cgtatcagcg agaacagtta gcgcatcgcc gatctgcccg acggtatccg
     3361 tcatcggggc gttttcagcc acggacgggt ttcgcatcga catcgcaagc agcgtattca
     3421 tggcgagcgc cttgctcgtg tcctgcgctg aagtcagcac tttcgtgtca gtcacggtcg
     3481 ccatcgcatc tttgagtcgc gtgagcacct gcccgctttg cgcgatcgag ccgtcaagtg
     3541 ttccggcaac gaggcttgtt gtgccggacg tatcggtcaa tacgctctgc atggtcgtac
     3601 caatcttctt cagatcggtg atgagcgttt tatccgattt gcccgctgcg ctgctgaatg
     3661 ttttgttcga cgcgagatca gttacggttg aattgttcgc atcgaacagc gtcaatgtgt
     3721 tgaatgttcc ctggggaaga tccacctgct gccgcccgat gaccgacgta tattgcgaag
     3781 tgcatatatt gtctgtaccg aatattttcc gcgtgaccag cgtaagatta taagaagcga
     3841 atatgtagta gaaagctgca taggtaggac cttctccgac aagcgaacct tcgataaacc
     3901 attcgtatag atcggcgctg cgatctgaca cggtgcaatt ggcaacaccg aacgcagtgt
     3961 cattcgggaa agaagtggta taggtgaacg ctgcatccac cgcctggttg accgttacct
     4021 ggaacgttgc gagctgtttt ttcattttgt tgctttcaaa cagcttgaac ggaacaattc
     4081 ccgttttgac ttccgttgag gaaggatcga actgccattg cccgtttttc aggctggctc
     4141 cttttccgtc gacgtaaagt gtggaaggat ctgctccgtc tgcgtctatt gtgaaattat
     4201 ccggcggact gtttttgcaa tactgcagcc tcggcaactg gaagatgatc gatccgcagc
     4261 aggccaggat gtccacctcg acgtacgtga cgacggaacg gttgttgctc ttgtccgtta
     4321 caagatagta gaagcggtcg gtgacggtgt cgctgatcgt tcccggcgta ttcgaagaat
     4381 caaatattgc agtaacgcca tccgtatcga gcgtgacgag gttgcctttt tcgctgaggt
     4441 aatctccttc cgggattgat ccgtcggatt tcaccagcgt tacggtaatg ttttcctgcc
     4501 agtcaagcca gctgttatcg ttggaaagaa gatcgatgtt cacttttctt ccgacaaggg
     4561 tcgctgcttt gtcaggatgt gtattgatca ggcggaaagc aggtgtttct acgatcacgc
     4621 gcagcgtgca gtaatccatt tccttcgttt ccgggttccg tacgctgagc aggaagaagt
     4681 cgacggtcgg atctgctttt tcatttttgt aaacgacaac ggcgcgtttg gcgctggtgt
     4741 cgatcgaaat gctgcctgtg ttcttgaagt catcaggcga atcggtttct tccagcacga
     4801 cgtccgccac aggaagaaga ggccgggcga gatagtaatc attgctcagc acgtcgatca
     4861 cgagtccgtc tgtcccgagg gcgagcaggt cgtcctgcag tatcgtgatc tcgctgtcca
     4921 gcgcaataag cggttgctgc agttcttttt tcgtgtcggt caggcacggc gagcagcaga
     4981 tgcccggcaa tgcaaaatcc gcgacgacgg gattgatggt tccgtcgtaa aaatttgttt
     5041 tggaataaac gagcagcaat gtgccgttct gcggaacgcc cgcgtagtgt tcgagcccgc
     5101 aatgttcttt cgcatactgg ctgaaaacgg gcgcgatcgt ttttgcggca atgcgctgat
     5161 cacgcaggta acggagaatt ccgagcttcg ggtagatgca gctgcagtcg agccagcgaa
     5221 gccggttgat gatgtcgcgg taaccgctgt attgtatctc ctgcgaaacg gtcatttccg
     5281 tgtcgtagaa gaactgctcg tttatttcca gcacgatctc gaaatagctc ctgtactgga
     5341 taacgaagtg ctgaagctgc ttgtattcct tcaggaaaat ctcatagtcg aaatcggcaa
     5401 gcaggttcgg gagccgtttt acaatgcttt tggaaacggg ctgagaaagc gagcacattt
     5461 tggtgaccag ctcggaaagc tgcaggaatg tggccgccga atagttcgct gtcacatacg
     5521 agggaacggc cgctttcccg aacgcatcga tcgttttctt tgccgctgcg gtcagaccgg
     5581 aaaacgacct gagcgtcagt tttcctgaaa acagtttcga gaacttctcg aaaataattt
     5641 tgtcatactt cacgggagcg ctcatttcct tgcaaagaat atcctgcaat gcacggacgc
     5701 agcagatgat ctcgttgcgc acgagcaggt aatcggcctg caggtcgtcg aacatgcagt
     5761 ggtccgattc acgcggatcg ggttgcaggc tgaggatcga agtatccgga tcggctgcat
     5821 cggcttccag cttgacgagc aggacgtcga aattcaggtt gcgttctttt ttcagcgcga
     5881 tgagatcgtt gcggacgttt tcgacccttt ttccggcgat tccctccacg cgcagcagcg
     5941 gtttgtcgag gatggaataa ttaagcgggc taatgatata cggaacggat gaataggtgc
     6001 ccgaaaaata accgggacga tcggcctgcc tgccgcgcaa tgttttttcc gggttccata
     6061 ccggcagcaa ctcggttttc tgtttgcttt cgctgtaata atacgggatg ctgcggtcgg
     6121 acagcaattg cattgcagaa ccatcgggtg tgatcagtgt tttgtcggtc ttccacgtgc
     6181 gcagcgaagg atcggtgaaa tcacacagca gcagcagcat tttccggaag agcatgcgcg
     6241 cattgttcag gtgatgatcc tgcgcatcga acggcggaac gttgaagaac acggtgcggt
     6301 aaatgtcgta gggttgcgtg accccgttct tcagccgccc gatggtgaga tgtttcggga
     6361 acggggaaag tgattttccg cacgactgga gaaaccgcc
//
LOCUS       DASTSF010000400         5576 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_613285,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000400 DASTSF010000000
VERSION     DASTSF010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5576
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_613285"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..476
                     /locus_tag="VFU15_11450"
     CDS             <1..476
                     /locus_tag="VFU15_11450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718445.1"
                     /translation="GRVFEGGKNLGPNMIPLRTLKSGIQYMLSRPTKLTALKDPDAEY
                     RVIKNYFEAVKLWQPDAWKNPSEYILLRGAGLWGICFIGANVIDKGLSLGKFDTDNLL
                     KILKSGRKWDWSKKGSFEGFSGRGGAVKISDMVTAELVDENNISVSELYKQIMKD"
     gene            complement(698..1534)
                     /locus_tag="VFU15_11455"
     CDS             complement(698..1534)
                     /locus_tag="VFU15_11455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718446.1"
                     /translation="MKKLGLCLGLLFSLNVSGQQQKYVADTLSPVQIVQLYVSPNGFP
                     GKMKFFCCELYQEWYADKTLGQQLPANVQRDVKVIYEDTIHAAVSVWLHDSVTSKDIY
                     FYLVHNQTWAVYAARSLAYTDVAVNQLKKMDSIPLADRGKTYTDAHGRTFAFEHDNLT
                     LWGGSDTNLVQFFRQNEKTFNAVQKRMTKKGYYGRNDTLVNGAMKDKKIKSLSQKILI
                     RGFQSDKLYPGAVFYMLGGVSDNSVGYMYQPDATKVPNMTEKHFILVRPLGNGWYLFK
                     TT"
     gene            1686..2747
                     /locus_tag="VFU15_11460"
     CDS             1686..2747
                     /locus_tag="VFU15_11460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009186406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase family protein"
                     /protein_id="HEU4718447.1"
                     /translation="MSKADKIRNFDPNSPGDPNSNIYGLPFSCEEAEVVIVPVPWDVT
                     VSYSDGTSKGPAAIFEASFQVDLYEPQINDAWKLGLAMEEISDEVSSRTEMLRAEAKT
                     VIDAITNGVDPSKDYSIGKILAQVNEGSAWLNSWVKKRCADWMEKGKLVCLLGGDHST
                     PFGFMQALAGKYESFGILQLDAHADLRKAFEGFTYSHASIMYNALTLPQVTKLVQVGI
                     RDFCQEEAELIRSSNGRVKTFFDRDLKHQGYTGVTWAEQVDEIIAHLPAHVYVSFDID
                     GLDPKLCPHTGTPVAGGMEFEQAAYLIQKLASSGKKIIGMDLNEVAPGPEGDEWDANV
                     GARMLFRMCNLMALSNGRF"
     gene            2750..3436
                     /locus_tag="VFU15_11465"
     CDS             2750..3436
                     /locus_tag="VFU15_11465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016776048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEU4718448.1"
                     /translation="MENYTAALGEFLSGFISETRKQKFHDILALRTRYLTVAVEDIYQ
                     PHNISAVLRSAECFGVQDVHIIGNRNKYNPNPDIVMGASKWLTLHHYTKTEDNTLSCI
                     RSLKEKGYRIVATTPHEEQASPETFDLEKGKAVLFFGTELEGISETVKREADEFLTIP
                     MYGFTESFNISVSAAICLYALTSRLRKSDKHWQLDETEKAELFLEWTRNTLNRPDLLE
                     EEFRKKAGLV"
     gene            3540..3746
                     /locus_tag="VFU15_11470"
     CDS             3540..3746
                     /locus_tag="VFU15_11470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein THX"
                     /protein_id="HEU4718449.1"
                     /translation="MQTTIQKNNNITTMGKGDRKSRKGKIWRGSYGNTRPRKPGKGTG
                     TAAKAAPKKKAAPKKSAKKASAEK"
     gene            complement(3783..4466)
                     /gene="pdxH"
                     /locus_tag="VFU15_11475"
     CDS             complement(3783..4466)
                     /gene="pdxH"
                     /locus_tag="VFU15_11475"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014680032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HEU4718450.1"
                     /translation="MAPRSNFIAMEKDINEYLKKMRHDFSVMKLDETMVDKDPFDQFG
                     SWFVAAVEAKVNEPNAMVLSTVSESGRPSSRVMLLRHFSGKGFSFFTNYNSRKGKEIE
                     KNPAAAILFFWPELERQVRIEGKLSKVSAAESDDYFLSRPRESRIGAWASPQSEMISG
                     RKALEDFLGNMKDKFADAEVIRPEWWGGFNLVPDRIEFWQGRPSRLHDRICYVRQDDG
                     TWRISRLAP"
     gene            4509..5078
                     /locus_tag="VFU15_11480"
     CDS             4509..5078
                     /locus_tag="VFU15_11480"
                     /inference="COORDINATES: protein motif:HMM:NF014659.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HEU4718451.1"
                     /translation="MLFPANKIKPAQGRLLVSEPFLHDDYFRRSVLLIAEHNEKGTVG
                     FILNKPLDVTLQEAVPGFPEFSGTAFFGGPVQRDQLYYVHTLGNVIPDSVQISPGLWW
                     LGDFDKVKEMMRRKEIGEKEIRFFIGYAGWEEGQLSRELGERSWYVSRGDREVVFSDA
                     HETLWTETVKSMGSEYSLIVNYPEDPSMN"
     gene            complement(5079..>5576)
                     /locus_tag="VFU15_11485"
     CDS             complement(5079..>5576)
                     /locus_tag="VFU15_11485"
                     /inference="COORDINATES: protein motif:HMM:NF024934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEU4718452.1"
                     /translation="FQVNRDGREQIVYIYSRGDIMGYRPLICGEPNPVSAMSLDETSY
                     YFIPKEDFLRILFSSPEFTRALLISLSYEFSVWANNITVFARQPVKSRVALGLLILEK
                     KFRREGAGEINLSREDFASYVGTVKETLVRVLQEFKKKKIIVSSGRRIKVLKPAALEE
                     IADFF"
BASE COUNT         1440 a         1464 c         1440 g         1232 t
ORIGIN      
        1 ccggtagagt ttttgaaggc gggaaaaacc tcggaccaaa tatgattcct ttgcgaactt
       61 taaaaagtgg cattcagtac atgttgtctc gtccaactaa actaactgca ttaaaagatc
      121 ctgacgctga atatcgagtt atcaagaatt actttgaagc ggtaaaactt tggcaacctg
      181 atgcatggaa aaatccatct gaatatatac tacttcgcgg agcaggtcta tggggaatct
      241 gtttcatagg tgcaaacgta attgacaaag gattgtcttt aggcaaattt gacacggata
      301 atctattgaa gatattaaaa tcaggtagaa aatgggattg gtcaaaaaaa ggtagtttcg
      361 aaggatttag cggaagagga ggtgccgtaa aaattagcga tatggtaacg gccgaattag
      421 tagatgaaaa caatatttct gtgagtgaat tatataagca aatcatgaag gattagaaaa
      481 acagcacata acagcaccag cccaaatttg gcggttcagt ggttaaatca gctttgtggt
      541 cggctgaaaa cgaatcgctc cgaaatcgcc accttcgggt agccgccaaa ccgttagcgc
      601 cagcacgaaa attatctgcc cctgatcatc tcccccattc caactttttg acctcccaac
      661 cttttaactt tccaacttcc caactgtata accgaattca cgtcgttttg aaaagatacc
      721 acccgttacc caggggcctg acgagaatga aatgcttttc cgtcatgttc ggaactttcg
      781 tcgcatcggg ctggtacatg tatccgaccg aattatccga aacaccaccg agcatgtaaa
      841 aaaccgcgcc gggatacagc ttgtccgact ggaaaccgcg tatgagaatt ttctgcgaca
      901 ggcttttgat cttcttgtct ttcatcgcac cgttcaccag cgtatcgttc ctgccgtaat
      961 accctttctt cgtcatgcgt ttctgcacgg cgttgaacgt cttttcgttc tggcggaaga
     1021 actgcacgag gttcgtgtcg gagccgcccc agagcgtgag gttatcgtgc tcgaatgcga
     1081 aggtgcgccc gtgcgcgtcc gtgtaggttt tcccgcggtc agcgagtgga atggaatcca
     1141 tcttcttcag ctggttgacc gcgacatcgg tatacgccag cgagcgcgcc gcatagacgg
     1201 cccaggtctg gttgtgcacg aggtaaaaat aaatgtcttt cgaagtgacg gaatcatgca
     1261 gccagacgga aacggcggcg tgtatcgtgt cttcgtagat cactttcacg tcgcgttgca
     1321 cattggcggg aagctgctgg ccgagggtct tgtcagcata ccattcctgg tacagctcgc
     1381 agcagaaaaa tttcatcttg ccgggaaaac cgttcgggga aacgtacagc tgtacgatct
     1441 gcaccggcga aagtgtatcc gccacgtact tctgctgctg gccggaaacg ttcagcgaaa
     1501 aaagcaggcc aaggcataaa ccgagtttct tcacgcgtta aagataaagg ttaagcgatt
     1561 cgtgcaaatc aacagtcaga cgggaaaatg ggcaagggtt gttttacccg gcggattatt
     1621 cattcctgcc ccccgtcctc aattattgat gattcaccat tacctttgcc gcgaaaaatt
     1681 tcaccatgag caaagcagac aagatcagga atttcgaccc caattctccc ggcgatccga
     1741 actcgaatat ttacgggctt cccttttcct gcgaggaagc ggaagtggtc atcgttcccg
     1801 ttccctggga cgtgacggtt tcctacagcg acgggacctc aaaaggaccg gcagccattt
     1861 ttgaagcctc tttccaggtc gacctgtatg aaccgcagat aaacgacgcg tggaagctcg
     1921 ggctggcgat ggaagaaatt tcagatgaag tttcctcccg tacggaaatg ctgcgggccg
     1981 aagcgaaaac agtcatcgat gcgatcacga acggcgtgga tccgtcgaaa gattattcga
     2041 tcgggaagat acttgcgcag gtgaatgaag gttcggcatg gctcaacagc tgggtgaaaa
     2101 agcgctgcgc ggactggatg gagaaaggca agctcgtctg cctgctgggc ggcgaccaca
     2161 gcacgccgtt cggctttatg caggcgctgg cggggaaata cgaatcgttc ggcattctcc
     2221 agctcgatgc acatgccgat ctccggaaag cgttcgaagg tttcacgtac tcgcacgcca
     2281 gcatcatgta caatgcgctc acccttccgc aggtcacgaa gctcgtgcag gtgggcatac
     2341 gcgatttctg ccaggaggaa gcggagctca ttcgatcttc aaacgggcgt gtgaaaacgt
     2401 tcttcgaccg cgacctgaag caccagggtt ataccggcgt cacctgggcg gaacaggtgg
     2461 acgagatcat cgcgcatctt ccggcccacg tatacgtcag cttcgacatc gacgggctcg
     2521 atccgaaatt gtgcccgcac accgggacgc ccgtggccgg cggaatggaa ttcgaacagg
     2581 ccgcgtacct gatccagaag ctggcttctt ccgggaaaaa gatcatcggt atggacctga
     2641 acgaagtggc gcccggaccc gaaggcgacg aatgggacgc gaacgtcggt gcgcgcatgc
     2701 tcttccgtat gtgcaacctg atggcgcttt cgaacggacg tttctgaaaa tggagaatta
     2761 caccgcagcg ctgggagaat tcctttccgg tttcatcagc gaaacccgga aacagaaatt
     2821 ccatgacatc ctcgcgttgc gcacccgtta cctcaccgtt gcagtggaag acatttacca
     2881 gccgcacaac atcagtgccg tgctgcgcag cgcggaatgc ttcggcgtgc aggacgtcca
     2941 catcatcggg aacaggaata aatacaaccc gaatcccgac atcgtcatgg gcgccagcaa
     3001 atggctgacg cttcatcatt acacgaaaac ggaggacaat acgctcagct gcatccgttc
     3061 gctgaaagaa aaaggctatc gcatcgtcgc gacaacgccg catgaagaac aggcttcacc
     3121 ggaaacgttc gatctcgaaa aagggaaagc ggttcttttt ttcggaacgg agctcgaagg
     3181 gatttccgaa accgtgaagc gggaagccga tgaattcctg acgatcccga tgtacggctt
     3241 tacggaaagc ttcaacatct ccgtttccgc cgccatctgc ctctatgcgc tgacgtcccg
     3301 tttacgcaaa agcgataagc actggcagct ggacgaaact gaaaaagcgg aactgttcct
     3361 cgaatggacg aggaacacgt taaaccgtcc cgacctgctc gaagaggaat tccggaagaa
     3421 agcagggctc gtgtaacaca gccgcattat tggaaagtca tcccgggcag tgaacggaaa
     3481 atgtacgggg aatgcggggc cgcaaaatcg ctgttcactt atcactgaat tatatacatt
     3541 tgcagacaac catccaaaaa aacaacaaca tcacaaccat gggaaaaggc gacagaaaat
     3601 cacgcaaagg caaaatctgg agagggtcat acggcaacac ccgcccgaga aagccgggca
     3661 aaggaacagg cacggcggca aaagccgcac cgaagaaaaa agcagctcct aaaaaatctg
     3721 ccaagaaagc aagcgctgaa aaataatcag caaaaaaccc ggaagcgatt ccgggttttg
     3781 ttttagggag caagccttga aatacgccac gttccgtcgt cctgccgcac atagcagatg
     3841 cggtcgtgca ggcggctggg acgtccctgc cagaattcga tgcggtcggg aacgaggttg
     3901 aaaccgcccc accattccgg ccgtatcact tcggcatcgg caaatttatc tttcatgttt
     3961 ccgagaaaat cttccagcgc cttcctcccg gagatcattt cactctgcgg cgacgcccac
     4021 gctcctatcc ggctttcccg cgggcggctc agaaaataat catccgattc agcagcggaa
     4081 actttcgaca gctttccttc gatgcgcacc tggcgttcca gctccggcca gaaaaaaagg
     4141 atcgccgcgg ccgggttctt ttcgatctct tttcccttgc ggctgttgta attcgtgaag
     4201 aacgaaaaac ctttcccgct gaaatgacgc agcagcatca cgcgcgagga aggccttccc
     4261 gattcgctca cggtcgagag caccatcgcg ttcggttcgt tcaccttcgc ttccactgct
     4321 gcaacgaacc acgacccgaa ctggtcgaac ggatctttat cgaccatcgt ttcgtccagc
     4381 ttcatcacgg aaaaatcgtg acgcatcttt ttcaggtatt cattgatatc cttttccatc
     4441 gcgataaaat tagaacgggg ggccataccg ccaaatgatc aggcgcatat tttttctaca
     4501 tttagcaaat gctgtttccg gccaacaaga tcaagcctgc acagggaaga ctgctcgtct
     4561 ccgagccttt cctgcacgac gattatttcc gccgctccgt gctgctgatc gccgagcaca
     4621 atgaaaaagg aaccgtcggg ttcatcctga acaagccact cgacgttacg ctgcaggaag
     4681 cggttcccgg tttcccggaa ttcagcggca ccgcattttt cggcggaccc gtgcagcgcg
     4741 accagctgta ttacgtgcat acgctgggca acgtcattcc cgattccgtg cagatctcgc
     4801 ccgggctctg gtggctgggc gatttcgaca aggtcaagga aatgatgcgc aggaaagaga
     4861 tcggtgaaaa agagatccgc ttcttcatcg gttatgcagg atgggaagaa ggacagcttt
     4921 cccgcgagct gggcgaacgc tcgtggtacg tttcgcgcgg cgaccgcgaa gttgtttttt
     4981 ccgatgcgca tgaaacgctg tggacggaaa ccgtgaagag catgggaagc gaatattcgc
     5041 ttatcgtcaa ttaccccgaa gacccttcga tgaactgatc agaagaaatc cgcgatttcc
     5101 tcgagcgcag ccggcttcag cacttttatc cttctcccgc tcgatacaat tatttttttc
     5161 tttttgaatt cctgcagcac gcggacgagg gtttccttca ccgtcccgac atacgacgcg
     5221 aaatcttcac gcgaaagatt tatttcgccc gctccttccc gcctgaattt cttttcgagg
     5281 atcagcagtc cgagcgcgac gcggcttttc acgggctggc gggcaaaaac ggtgatatta
     5341 ttggcccaga ccgaaaattc atagctgagc gaaataagca gcgcgcgggt gaattcggga
     5401 gaagaaaaaa ggatgcgcag aaaatcttcc ttgggaatga aataatagga tgtttcatcg
     5461 agggacattg cagaaacagg gttcggctca ccgcagatca gcgggcggta tcccataata
     5521 tcgccacggg aatagatgta cacgatctgt tcgcgcccgt cgcggttcac ctggaa
//
LOCUS       DASTSF010000401         3387 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1396184,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000401 DASTSF010000000
VERSION     DASTSF010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3387
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1396184"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(438..1541)
                     /locus_tag="VFU15_11490"
     CDS             complement(438..1541)
                     /locus_tag="VFU15_11490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718453.1"
                     /translation="MIRFILPLLGLCSSLFAGTTLHRDAAGDTVKFSIATALLKDGTR
                     IWGMLETESSDTVVINDFNAGPLSLARSSIREIEITPVDGKVMIETVNGTIYYGEIVG
                     MTGGVLMIRSDLIGVFPIQANTISKISVSAAYVSRKGGTWFTNPNATRYFFAPSAIPL
                     KKKEGYFQNAYLLANSVNVGITDNVTVGGGIVIPLLFYVTPKISFRASKNFYLGGGVL
                     FTQSFIKDFDLSAGIVYGLATYGNYEHNVTLGSGYGFASFNTEYKTTPMPIVTINGMT
                     RVAKKVSLVTENWLIPRGGYNKEVSTITPAGEVMMETVYEKKNFYSGAASFGLRFMPG
                     TRTSVDFSVVGILTDPGQGNLVLPYLDFVYKFN"
     gene            complement(1647..2744)
                     /locus_tag="VFU15_11495"
     CDS             complement(1647..2744)
                     /locus_tag="VFU15_11495"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEU4718454.1"
                     /translation="MVITTDSFFRKLLILVILIAGLYFAKAFLIPISIGTLLAMLFLP
                     FCKWLEKKHVSRGLASAICLLALMLAVAGVTGLLGWQFFALTKDLDQIKQKLLEIIAN
                     IQGYIFSHAGISPEQQSKFIGNQQPFVTRMIQRAANSLTTISVQAVLVMVYVLFLLYY
                     RSHFRNFLLQLVPPAKKEETDKIIYRVAQVSQQYLVGLSKMIVCLWIMYGVGFSLLGV
                     KNALFFAFLCGLLEIVPFVGNITGTSITVLVSAAQGASPALLAGIVATYSSIQFIQGW
                     VLEPLIVGPQVKINPLFTIIALVVGELVWGIPGIFLAIPVLAMFKIVCDHVESLKPLG
                     FLIGETKAGRSEPWFIRQAKKWYSKKKWNKE"
     gene            complement(2950..>3387)
                     /locus_tag="VFU15_11500"
     CDS             complement(2950..>3387)
                     /locus_tag="VFU15_11500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014021510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HEU4718455.1"
                     /translation="SLFIAGPFALGAAMFSLSISRGKEAKLEQIFQGFNRYTTSLAAY
                     LLIVAYVLLWLLLLIVPGIIAALSYSMTFYILADDPTIKAEDALKKSKAMMDGHKLKL
                     FYLYLWFLLLGLLCILTLGIGFLWLIPFANVTTAKFYDDIKEG"
BASE COUNT          872 a          860 c          827 g          828 t
ORIGIN      
        1 gattgacggc gctggtgctg tcttgcccga aaaatgcgat ctgcccgcgc ccctttccga
       61 aagaaaagca gagcagcaaa ctcatgaatg aacggacaat tatccggcgc cgactcatgg
      121 tgaggcgttt ttttaactcg taaaaccact tgctgtccct gcagtataaa aagccagtca
      181 gggtcccggg gctagctttt taattctcat caaaaaaata tccggaggaa tggatatgat
      241 taattcagta attattgatg agcatcaaat attaaaacta atgtatgtcc atttttatga
      301 taaagtcaat actcgcaggt tgtttttctg ttatatcaca agtatcttgt tgttaatcaa
      361 catgaaatat taataaaaaa attactcttt tataatttca tatgagtgtt cttagtgatg
      421 gaagattgcc gaaccactca attgaatttg taaacaaaat cgagataagg taaaacgaga
      481 ttgccctgcc cgggatcggt aagtatgccc accacggaaa aatcgacgct cgtgcgcgtg
      541 ccgggcataa atctcaatcc gaacgacgcc gcgcctgaat agaaattttt cttttcgtaa
      601 actgtttcca tcatcacttc gccggccggt gtgatcgtcg aaacttcttt attatacccg
      661 cctcttggta tcagccagtt ttcggtgaca agcgaaactt ttttcgccac gcgcgtcatc
      721 ccgtttatcg taacgatggg catcggggtg gttttgtatt ccgtgttgaa actggcgaag
      781 ccataaccgc tccccagcgt cacgttgtgc tcgtagttgc cgtaagtggc cagcccgtac
      841 acgatgccgg cgctcagatc aaaatctttt ataaaggatt gggtaaacaa aactccgccg
      901 cctaaataga aattcttcga agccctgaac gatattttcg gggtcacata gaacagcagg
      961 gggatcacta ttcctccgcc gactgtaaca ttgtccgtga tccccacgtt caccgaattg
     1021 gccagcaggt aagcgttctg gaaatagcct tctttctttt tcagcgggat ggcgctcggc
     1081 gcaaaaaagt aacgtgttgc gttgggattg gtaaaccacg tgccgccttt cctgcttaca
     1141 tacgccgcgg aaaccgagat tttcgaaatg gtattggcct ggatcgggaa aacaccgatg
     1201 agatcagacc tgatcatgag aactcctccc gtcatcccga cgatctcccc gtagtagata
     1261 gttccattca cggtttcgat catcactttc ccgtccaccg gggttatttc tatttccctg
     1321 atgctgcttc tcgcaaggga aagcggtcct gcgttgaaat cattaatgac caccgtatcg
     1381 ctgctttcgg tttcgagcat tccccatatc cgcgttccgt ctttcagcaa cgcagtggcg
     1441 atgctgaact ttacagtatc tcctgcggcg tcccggtgca acgtcgttcc ggcgaaaaga
     1501 gagctgcaga gtcccagcag cggcagtatg aatcggatca catatttcat ggcgataagg
     1561 ttgtttccgg cggatcgcac gagaaataac atgcgcgcat tacgcaggca cgcgcacgct
     1621 ttcccgcaac gatccccggt tgttttttat tctttgttcc acttcttctt gctgtaccat
     1681 ttttttgcct gccggataaa ccatggttcg cttcttcctg ctttcgtttc accgataagg
     1741 aatcccagcg gcttcagcga ttccacgtga tcgcagacga tcttgaacat cgcgaggacc
     1801 gggatagcga ggaagatgcc gggaatgccc cataccagtt cgcccaccac cagcgcgatg
     1861 atcgtgaaga gcgggtttat ttttacctgc ggacccacga taagcggttc gagcacccag
     1921 ccctgaatga actggatgct gctgtacgtc gcgacgatgc cggcaagcaa agccgggctt
     1981 gcgccctggg ccgccgaaac gaggacagtg atggaagtgc cggtgatgtt cccgacgaac
     2041 ggcacgatct ccagcaggcc gcagagaaaa gcgaagaaaa gcgcgttttt aactcccaat
     2101 aaagaaaacc ccacgccata catgatccag agacacacga tcatttttga aagcccgacc
     2161 aggtattgct gcgacacctg tgcaacgcgg taaatgatct tgtctgtttc ctcttttttg
     2221 gcaggcggta caagctgcag caggaaattc ctgaagtgac tgcggtaata cagcaggaac
     2281 aagacataca ccatgaccag cacggcctgc acggaaatcg tcgtcagcga attggccgcg
     2341 cgctggatca tccgcgtgac aaaaggctgc tggttgccga tgaatttcga ctgctgctcg
     2401 ggagaaatgc cggcgtgcga aaaaatgtat ccctggatgt tcgcaatgat ctcgagtaat
     2461 ttctgcttga tctggtccag gtctttggtc aatgcaaaaa actgccagcc cagcagcccg
     2521 gtcacaccgg caaccgcgag catcagcgcc aggagacaga tcgcggatgc aagtccgcgg
     2581 ctcacgtgtt ttttttcaag ccatttgcag aagggcaaaa acagcatcgc aagaagagtt
     2641 ccgatggaaa tgggaatgag gaacgctttt gcgaaataaa gtcctgcaat gaggataacc
     2701 aggatgagca gcttcctgaa aaatgaatcg gtggtaatga ccataatctt ccggtttccg
     2761 ggttgcggtg tccacacaaa aaccgcctgc aatttcgcag ggttgcgttt cgcgctgcat
     2821 gactgcaatc acgggagatc ttgaacaata tcatcgccgt gcaattgccg ggaagcattt
     2881 ttcatcctgc aaaaaatgaa acagcaaagg cgatcccgga atgcgccgcc tttcatacgt
     2941 cgtttttttt caaccttctt ttatgtcatc gtaaaatttc gccgtcgtca cattggcaaa
     3001 cgggatgagc cagagaaaac ctattcccag ggtcaggatg caaagcaatc ccagcagcag
     3061 gaaccataaa tacaggtaaa acaacttcag cttgtgcccg tccatcatcg ccttgctttt
     3121 tttcagcgcg tcttcagctt tgatcgtggg atcgtcggcg agaatataga atgtcatcga
     3181 ataggaaagt gctgcgatga tcccgggaac gatcagcagg agaagccaca gcaggacata
     3241 cgcgacaatc agcaggtatg ccgcgaggga agtcgtgtac cggttaaatc cctggaagat
     3301 ctgttccagc ttcgcttcct ttccccgcga aatggaaagc gagaacatcg cggcgccgag
     3361 cgcaaatggt ccggcgatga ataaaga
//
LOCUS       DASTSF010000402         4721 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1904362,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000402 DASTSF010000000
VERSION     DASTSF010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4721
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1904362"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..462)
                     /locus_tag="VFU15_11505"
     CDS             complement(<1..462)
                     /locus_tag="VFU15_11505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011585458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HEU4718456.1"
                     /translation="MTMGASSLYDMVVIGASAGGLYALSFLFEALPEDYPVPIAVVQH
                     RSKDQKELLEDVLRHKCLIAVQQAEEKESIRPGVVYIAPPDYHLLIESDRTFSLTSDE
                     LVNYSRPSIDVLFESASAVFGARLAGIILTGANADGAAGISALSKRGGMTIA"
     gene            complement(459..1274)
                     /locus_tag="VFU15_11510"
     CDS             complement(459..1274)
                     /locus_tag="VFU15_11510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009594100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HEU4718457.1"
                     /translation="MNFDSRELRDLLDSIRFVYGYDFTQYAEASVKRRISHFVTNRKF
                     EGLDAMGKAILRDEKLFEEFVRDMSVTVTEMFRDPPVYKRMRTNVMKRLATYPFIKIW
                     IAGCATGEEVYSVAIMLSEENLLGRSVIYATDINQHSLQVAKEGVYDVEKMKEYTMNY
                     QKAGGKRAFSEYYKAKYNSVMFDRELRTNIVFAPHNLATDKSFNEFQLIICRNVLIYF
                     NQQLQNRVIGLFYESLCPFGFLGLGNKESLLFSDKRKFFEEVDHKGKIFMKAE"
     gene            complement(1277..1657)
                     /locus_tag="VFU15_11515"
     CDS             complement(1277..1657)
                     /locus_tag="VFU15_11515"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU4718458.1"
                     /translation="MEKLQNKSILIVDDDDRNTFALVSYLETLGVKITVAGNGREALT
                     MLEEGLESDLILLDMMMPVMDGYETLDFMRQNSMLDRFPVIAVTARAMKGDREKCLEA
                     GASDYISKPVDMRLLIEKMNRWIH"
     gene            complement(1669..>4721)
                     /locus_tag="VFU15_11520"
     CDS             complement(1669..>4721)
                     /locus_tag="VFU15_11520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU4718459.1"
                     /translation="QVEMEKTALELGQQIDCLNNAAIVSITDAGGDIIYVNDTFCKIS
                     KYSREELIGKNHRLLKSGRQPDGLFVGMWKAVSIGRTWNGEILNKAKDGTFYWVDTTI
                     TPFRGLDGKIEKYVAIRFDITSMKNQKTEIEAVMSAIYRSNSAVEFDLDGNILKANDI
                     FLEMMGYTENEILGKHHSMFVEKEFAGTKEYRDFWNALRKGKYQEDEFMRITKSGEPI
                     WIKGNYNPILDSQGKPYKVLKIATDITLIRKQSMELAAQTEELEAQQEELRQSNEELM
                     EKTILLEKSETELKSQQEELQQTNEELEEKANLLEEQKDSLENAKMDLENKARELEVA
                     GKYKSEFLANMSHELRTPLNSILILAQLLSENKNNQLSEKEVDFARNIYNSGTDLLSL
                     INEILDLSKVESGKMELDIINVPFSDISADISAMFTEVAKNKSIGFSIETDRKLAKDA
                     LSTDKQRLEQILRNLLSNAFKFTGKGGAVILTTGRPAAGTHFRNKTLGAKKDIIAFSV
                     TDTGIGIPAEKQSIIFEAFQQADGSTKRKYGGTGLGLSISRELAATLGGEIHLQSEEG
                     KGSTFTLYLPQQFDATLIRLVERNVEVRESVSGLPAKKPVERLPLLSSESSDDRYDIK
                     ENDRSVLIMEDDPDFAQVLLTFARERKYKGIIASEGNTGLSYARYYKPDAILLDMNLP
                     VIDGADVLKQLKNDPSLRHVPVQIISGYDRRKEGLQLGAFDFLRKPISKDDLRNAFDK
                     IEKFLSKKLKKLLIVEDNEKQNNAIRELVGNGDVKSFAAYSGSEAYRMMNSENFDCVI
                     VDLGLPDMTGFDLLEKIKASDTLKKIPIIVYTGKDLTKEESVRLNKLANTVVLKTVDS
                     NERLLDETTLFLHRVESRLPKEKQNIIRRLHKTDEVLKNKTVLVVDDDIRNIYSLTNV
                     LEEEGINCLTAENGKAALKMLKEHPGIDIILMDVMMPEMDGYEATREIRNISRFSKLP
                     IIALTAKAMKGDREKCLNAGMSDYITKPVNIEQLLSLMRVWLYR"
BASE COUNT          899 a         1286 c         1202 g         1334 t
ORIGIN      
        1 tgcaatggtc atcccgcctc ttttcgataa cgcggagatc ccggccgcgc cgtcggcgtt
       61 tgcaccggta agaataatgc cggccagccg cgctccgaat accgcggaag ccgattcgaa
      121 cagcacatcg atcgagggac ggctgtaatt caccagctca tccgacgtca gcgagaacgt
      181 acggtcgctt tcgatcagca ggtgataatc cggcggcgcg atataaacga cgccgggacg
      241 aatgctttct ttttcttccg cctgctgcac ggctatcagg cacttgtgac gcaatacgtc
      301 ttcgagcaat tctttctggt ctttcgaacg gtgctgcacc acggcaatgg gcaccgggta
      361 atcctcgggc aacgcttcga acaggaatga aagcgcgtaa agtccgccgg cagaagcgcc
      421 gatcacgacc atgtcatata aagaggaggc gcccatcgtc attctgcttt catgaaaatt
      481 ttgcctttgt ggtctacctc ttcgaagaat ttccgtttgt cggaaaagag cagcgattcc
      541 ttgttgccga gcccgagaaa accgaaaggg cagagacttt cgtagaaaag accgatcacg
      601 cggttctgca gctgctggtt aaaatagatc agcacgttgc ggcagatgat aagctggaat
      661 tcgttgaacg atttatctgt tgcaaggtta tgcggtgcga aaacgatatt cgttctcagc
      721 tcgcggtcga acatcaccga gttgtatttc gctttgtaat attccgagaa agcgcgtttc
      781 ccgccggctt tctggtagtt catggtgtac tctttcattt tttccacatc gtaaacgcct
      841 tctttcgcca cctgcagcga atgctggttg atgtcggtgg cgtagatcac ggaacggccc
      901 aggaggtttt cctcgctgag catgatcgcc accgaataaa cctcttcccc cgtggcgcag
      961 ccggcgatcc atattttgat gaacgggtag gtggcgagcc gcttcatcac atttgtgcgc
     1021 attcttttgt agacgggcgg atcacggaac atttcggtca ccgtgaccga catatcacgc
     1081 acgaattcct cgaagagctt ttcgtcgcgc aggatcgctt ttcccatcgc gtcgagcccc
     1141 tcgaatttcc ggttggtcac gaagtggctg atccttcttt tcacggaagc ttcggcgtac
     1201 tgcgtgaaat cgtatccata cacaaaccgt atggagtcga gcaggtcacg gagttcgcgg
     1261 ctgtcgaagt tcattgtcag tggatccagc ggttcatttt ttcgatcagc aacctcatgt
     1321 cgaccggctt ggaaatataa tcgcttgcgc cggcctccag gcatttttcc ctatcgcctt
     1381 tcattgctct ggccgttacc gctatcacgg gaaaacggtc cagcatactg ttctgccgca
     1441 tgaagtccag cgtttcatat ccatccatca cgggcatcat catgtcgagc aggatgaggt
     1501 cgctttccag tccttcttcc agcatcgtca gcgcttcgcg gccgttgccg gcaacggtga
     1561 ttttcacccc gagcgtttca aggtaactga cgagcgcgaa cgtgttgcgg tcatcatcgt
     1621 ccacgatcag gattgatttg ttctgcagct tttccattgg gccagggttt aacggtaaag
     1681 ccatacgcgc atgagcgaaa gcagctgctc gatgttcacc ggtttggtaa tgtaatccga
     1741 catgcctgca ttgaggcatt tttcacggtc gcccttcatc gcttttgcgg taagcgcgat
     1801 aatgggcagc ttgctgaacc ggctgatgtt ccggatctcg cgcgttgcct cgtagccgtc
     1861 catttcgggc atcatcacgt ccatgagtat gatgtcgatg ccgggatgtt ccttcagcat
     1921 tttcaatgcc gctttcccgt tttccgcggt gaggcagttg atgccttctt cctcgagcac
     1981 gttcgtgagc gaataaatgt tccggatgtc gtcgtccacc acgagcacgg ttttgttctt
     2041 cagcacttca tccgtcttgt gcagcctgcg gatgatgttc tgcttttctt tcggcaggcg
     2101 cgattcgaca cgatgcagga acagcgtcgt ttcgtcgagc aggcgttcgt tcgagtcgac
     2161 ggttttcagc accaccgtat tggcgagctt gttcagccgg acgctttctt ccttcgtaag
     2221 atctttgccg gtgtaaacga tgatagggat ctttttcaac gtgtcgcttg cttttatctt
     2281 ttcgagcaga tcgaaaccgg tcatgtcggg cagaccgaga tcgacgatga cgcaatcaaa
     2341 attctcgctg ttcatcatcc tgtaggcttc gctgcccgag taggccgcga acgatttcac
     2401 gtcgccgttg ccgacaagtt cgcggatcgc gttattctgc ttctcgttgt cctccacgat
     2461 cagcagcttc ttcagctttt tgctgaggaa cttttcgatc ttatcgaacg cgttgcgcag
     2521 gtcatctttc gaaataggtt tgcgcaggaa atcgaaagcg ccaagctgca gtccttcttt
     2581 tctccggtcg tagccggaaa tgatctgcac cgggacgtga cggagcgaag gatcgttctt
     2641 cagctgcttc agcacatccg caccatcgat caccggcagg ttcatgtcga gcaggatggc
     2701 gtcgggtttg taataacggg cgtagctcag tcccgtattt ccttccgaag cgatgatccc
     2761 tttgtatttg cgctccctcg cgaacgtaag cagcacctgc gcaaaatcgg gatcgtcttc
     2821 catgatgagc acgctccggt cgttctcttt tatgtcatag cggtcgtcgg aactttcgct
     2881 gctcagcagc ggcaatcgtt caaccggttt tttggcaggc agtccggata cgctttccct
     2941 tacttcgacg tttcgctcca ccagccggat aagtgttgcg tcgaactgtt gtggcaggta
     3001 cagggtgaac gtgcttcctt ttccttcttc gctttgcagg tgaatttccc cgccgagcgt
     3061 agcggccagc tcgcggctga tggaaagacc gagtcccgtt ccgccgtatt tgcgcttcgt
     3121 ggaaccgtcg gcctgctgga acgcctcgaa gatgatggat tgcttttctg cgggaatgcc
     3181 gatgccggtg tctgtcacgg aaaaggcgat gatgtccttt tttgccccga gcgttttgtt
     3241 gcggaagtgc gttcccgcag ccggtcttcc ggtcgtcagt ataaccgctc ctcccttgcc
     3301 ggtgaacttg aatgcattcg acaggaggtt ccgcaggatc tgttcgagac gctgcttgtc
     3361 ggtcgaaagc gcatctttcg ccagcttcct gtcggtctct atgctgaagc cgatcgattt
     3421 gttttttgcg acttccgtga acatggcgct gatgtccgcg ctgatgtcgg agaacggaac
     3481 attgatgata tcgagctcca ttttcccgga ttccactttc gagaggtcga gaatttcatt
     3541 gatcagcgaa agcagatctg ttcccgagtt gtaaatattc ctggcgaaat caacttcttt
     3601 ttcgctcagc tggttgttct tgttttcgga aagcagctgc gccaggatga ggatgctgtt
     3661 gagcggcgtg cgcagctcat gcgacatgtt ggccaggaat tccgatttgt attttccggc
     3721 tacctccagc tcccgcgctt tgttttcgag atccattttc gcattttcaa gactatcctt
     3781 ctgctcttcg agcaggttgg ctttttcttc cagctcctcg ttggtctgct gcagttcttc
     3841 ctgttgcgat ttcagctcgg tttccgattt ttcgagcagt atggtttttt ccatcagctc
     3901 ttcattcgac tgcctcagct cttcctgctg cgcttccagc tcttctgtct gtgccgccag
     3961 ttccatggat tgctttcgga tcagcgtaat gtccgtcgcg atcttcagca ctttgtacgg
     4021 ttttccctgc gagtcgagaa tgggattgta atttcccttg atccagatcg gttccccgct
     4081 tttggtgatc cgcatgaatt catcctcctg gtatttccct tttcgcaggg cattccagaa
     4141 atccctgtat tctttcgtac cggcaaattc tttctcaacg aacatgctgt ggtgttttcc
     4201 caggatctca ttttccgtat agcccatcat ttccaggaaa atatcgttcg ctttcaggat
     4261 gttcccgtcg agatcgaatt ccactgccga attcgaccgg tagatcgccg acatgacggc
     4321 ttcgatctcg gtcttctggt tcttcatcga cgtgatgtcg aaacggatgg cgacatactt
     4381 ttcaattttt ccgtccagtc ccctgaaagg cgtgatcgtc gtatcgaccc aatagaatgt
     4441 gccgtccttt gccttgttca gtatttcccc gttccaggtc ctgccgatgc tgactgcctt
     4501 ccacatgcct acaaacaaac cgtccggctg ccgtccggat ttcaggaggc ggtggttttt
     4561 cccgatcagc tcttcgcgcg aatatttcga gatcttgcag aacgtatcat tgacatagat
     4621 aatatcgccg ccggcatccg taatggaaac gatcgctgcg ttgttcaggc agtcgatctg
     4681 ctgcccgagc tcaagcgcgg ttttttccat ttcaacctgc t
//
LOCUS       DASTSF010000403         2826 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_281897,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000403 DASTSF010000000
VERSION     DASTSF010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2826
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_281897"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..328)
                     /locus_tag="VFU15_11525"
     CDS             complement(<1..328)
                     /locus_tag="VFU15_11525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014085033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HEU4718460.1"
                     /translation="MPDLVAIVGRPNVGKSTLFNRLTRSREAIVDSVSGVTRDRHYGY
                     AEWNGRKFSVVDTGGYVEGSDDVFEEQIRSHVRIAVEEASILLFVVDALEGITPLDLE
                     VADIVRR"
     gene            complement(407..1285)
                     /gene="era"
                     /locus_tag="VFU15_11530"
     CDS             complement(407..1285)
                     /gene="era"
                     /locus_tag="VFU15_11530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006259644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEU4718461.1"
                     /translation="MAHKAGYVNIFGNPNAGKSTLMNALVGERISIVTSKAQTTRHRI
                     LGIVNGDDYQVIYSDTPGILKPRYKLQEGMMSFIGEALSDADVILLVVDLSDDTPMAE
                     ETFAKIRKTKAPLMILLNKCDLVSPETIAAKTEEWKKELPAAELFAVSALKGTNLEKI
                     FGRIIALLPEHPGYFPKDQLTDRSERFFISEIVREKILLNYHQEIPYSVEVVVEEFKE
                     NETLVKIRANIFVSRETQKAILIGNKGSAIKQLGIDSRADIEKFLGKKVFLELFVKVD
                     KDWRDTEKGLRRYGYL"
     gene            complement(1334..2407)
                     /locus_tag="VFU15_11535"
     CDS             complement(1334..2407)
                     /locus_tag="VFU15_11535"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009193987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HEU4718462.1"
                     /translation="MNVSFKSQGNAFFQALRSGVNAYFKTNGIRSTGNFQLYLKTVII
                     FLALAALYTWVVFFTPGVWLSVAICSFLGLCAALIGFNVMHDGSHGSYSRNKTVNRIM
                     AYSLNLLGGNAFFWTQKHNQNHHTYTNVEGLDDDIDLKPFLRVHHDQPKKKIHRYQHI
                     YFFVLYGVTYLFWVYYRDFLKYFTGKIAENTKLSKMSLREHIVFWVSKLVHIGVFLVI
                     PMLFAGVRETIIGYLIMAFVMGFTLAIVFQLAHLVEGAEFTAPEPGKDTNIETEWAIY
                     QVNTTVNFATRNRVLSWLLGGLNYQMEHHLFPKISHVHYPAVHRIVKQVCTEYNVSYI
                     EHPTMISAIRSHIRHLRALGKAD"
     gene            2611..>2826
                     /locus_tag="VFU15_11540"
     CDS             2611..>2826
                     /locus_tag="VFU15_11540"
                     /inference="COORDINATES: protein motif:HMM:NF025734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding domain-containing protein"
                     /protein_id="HEU4718463.1"
                     /translation="MKRFKKILWALPAVLVIIQFIRPAKNNSAAVPAQQVSAVFNVPA
                     GVDSALRVSCYDCHSNKTHYPWYAEVQP"
BASE COUNT          680 a          742 c          743 g          661 t
ORIGIN      
        1 tgcggcggac gatgtccgcc acttccaggt cgagcggcgt gatgccctcc agcgcgtcca
       61 ccacgaagag aagaatggac gcttcttcaa cggcgatgcg cacgtgcgaa cggatctgct
      121 cttcgaaaac gtcatcggaa ccttccacgt acccgcccgt atcgacgacg gaaaacttcc
      181 gcccgttcca ttcggcatag ccgtaatgac ggtcgcgcgt aacgccggac actgaatcga
      241 cgatcgcttc gcgcgagcgt gtgaggcggt tgaacagtgt tgattttcct acgttcggac
      301 gtccgacaat agcaacgaga tctggcatgg cgcaaaagta ggtatcaaat ttggaattgt
      361 tcccttcagc ggttgacggg aaaaacggtg tgatccggaa aaagatttac aggtaaccgt
      421 aacggcgaag acccttttcc gtgtcgcgcc agtccttgtc gactttcacg aacagttcga
      481 ggaagacttt cttgccgagg aacttttcaa tgtccgcacg tgaatcgatg ccgagctgct
      541 tgatggcgga acctttgttg ccgataagaa tggccttctg tgtttcccgc gaaacgaaaa
      601 tattcgcacg gatcttcacg agcgtttcgt tctctttgaa ttcttccacc accacttcca
      661 ccgaataggg aatttcctgg tggtaattca gcaggatctt ttcacgcacg atctcggaaa
      721 tgaaaaaccg ttcggagcgg tccgtgagct ggtccttcgg gaaatagccc gggtgctccg
      781 gcagcagtgc gatgatccgc ccgaagattt tttccaggtt cgttcctttc agcgcggaca
      841 ccgcgaacag ttcggccgcg ggaagctctt ttttccattc ctccgttttc gcagcgatcg
      901 tttcgggaga aacgaggtcg catttgttca gcaggatcat cagcggcgct ttcgtcttcc
      961 ggatcttcgc gaaggtttct tccgccatgg gcgtatcatc cgaaagatcg acaacgagga
     1021 gaatgacatc cgcatcggac aacgcttcgc cgatgaagct catcattcct tcctgtagtt
     1081 tgtaccgtgg ttttaagatc cccggggtat cggaatagat cacctggtaa tcgtcgccgt
     1141 tgacgatgcc gaggatacgg tgacgcgtgg tctgcgcttt cgaagtgacg atggaaatgc
     1201 gttccccgac gagcgcattc ataagcgtgc tcttccccgc attcgggttc ccgaaaatat
     1261 tgacgtatcc ggccttgtgt gccatggaag cagaaacaaa tatcggcgta aaagaagaaa
     1321 cgggaaaagc ggatcagtct gctttcccga gcgcacgcag gtgccgtatg tgcgaacgga
     1381 tggccgagat catcgtagga tgctcgatgt aggaaacgtt gtattccgtg cacacctgct
     1441 tcacgatgcg gtgaacagcg ggataatgca cgtggctgat cttcgggaaa aggtgatgct
     1501 ccatctggta gttgagtccg cccagcagcc agctcagcac gcggttgcgc gtggcgaaat
     1561 tgaccgtggt gttcacctgg tagatcgccc actcggtttc gatgttcgtg tctttcccgg
     1621 gttcgggcgc cgtgaattca gcaccttcca caagatgcgc cagctggaaa acaatggcca
     1681 gcgtgaaccc catgacgaac gccatgatca ggtagccgat gatcgtttcc cgcacgcctg
     1741 cgaacaacat gggaataacc aggaagacac ctatatgcac cagcttggaa acccagaaca
     1801 cgatgtgctc gcgcaggctc attttcgaaa gcttcgtatt ctcggcgatc ttgcccgtga
     1861 aatatttcag gaaatcgcgg taatacaccc agaagagata ggtgacgccg tacagcacga
     1921 aaaaataaat atgctggtag cggtgaattt ttttcttcgg ctgatcatga tgaacgcgaa
     1981 ggaacggttt aaggtcgatg tcatcgtcca gcccttcgac attcgtatac gtgtggtgat
     2041 tctggttgtg cttctgcgtc cagaaaaaag catttccgcc aagaagattc agcgaatacg
     2101 ccatgatcct gttcacggtc ttgttccgcg aatagcttcc gtgcgaaccg tcgtgcatga
     2161 cgttgaaccc gatgagcgca gcgcacagac cgagaaagga acagatggca accgacagcc
     2221 agaccccggg tgtgaaaaac accacccagg tatagagtgc ggcgagcgcc agaaaaatga
     2281 taacggtttt caggtaaagc tgaaaattac ccgtactgcg tataccgttg gttttgaaat
     2341 aagcgttaac gcccgaccgg agcgcctgga aaaacgcgtt gccctgggat ttgaaagaaa
     2401 cgttcaatgc tatccttttt gagaatgatg ccgaaagtac acattaatcg gtctggaaaa
     2461 actgcattga cgtttattgg ctgttaagaa tacgccggag ccgttccggt tttaccggac
     2521 aaaagccggg aaatacccga ccgatctgtt tttcatcccc atcaatcgct cagccgtaat
     2581 acttctactt ttgcggaaat aattgttcac atgaagcgtt tcaaaaaaat tctctgggcg
     2641 ctgccggccg tcctcgtcat catccagttc atacgtccgg cgaaaaataa ttctgccgcc
     2701 gttcctgcac aacaggtcag cgcagtgttc aatgttcccg ccggggtcga cagcgcactt
     2761 cgcgtttcct gctacgactg ccacagcaac aaaacgcatt atccgtggta tgcagaagta
     2821 cagccc
//
LOCUS       DASTSF010000404         6812 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1576478,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000404 DASTSF010000000
VERSION     DASTSF010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 6812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6812
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1576478"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..535
                     /gene="map"
                     /locus_tag="VFU15_11545"
     CDS             <1..535
                     /gene="map"
                     /locus_tag="VFU15_11545"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013453591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEU4718464.1"
                     /translation="KEGDVVSVDCGVVMNGFYGDSAFTFPVGEVNPEVMRLLRVTREC
                     LYKGIEKAVAGGRIGDISEAVQQHAEKNGFSVVRELVGHGVGKHLHEAPEVPNFGRRG
                     NGPKLAEGLVIAIEPMINLGKKEVRQERDGWTITTADGLPSAHYEHTIAIHKGKADIL
                     SSFDEIEQVLKEKQIVI"
     gene            544..762
                     /gene="infA"
                     /locus_tag="VFU15_11550"
     CDS             544..762
                     /gene="infA"
                     /locus_tag="VFU15_11550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEU4718465.1"
                     /translation="MAKTPSIEQDGTIIEALSNAMFRVELENGHVITAHISGKMRMHY
                     IKILPGDKVKVEISPYDLTKGRISYRYK"
     gene            840..959
                     /gene="rpmJ"
                     /locus_tag="VFU15_11555"
     CDS             840..959
                     /gene="rpmJ"
                     /locus_tag="VFU15_11555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018106937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEU4718466.1"
                     /translation="MKVRTSIKKRSADCIVVRRKGRLYVINKKNPKFKQRQKN"
     gene            1002..1379
                     /gene="rpsM"
                     /locus_tag="VFU15_11560"
     CDS             1002..1379
                     /gene="rpsM"
                     /locus_tag="VFU15_11560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011586488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEU4718467.1"
                     /translation="MARIAGIDLPKNKRGEVGLTYIFGIGRNTARRILTEAGVDFDTK
                     VQDWNDDQLNKIRSVISEKIKVEGALRSEVQLNIKRLIDVGSYRGMRHRNGLPVRGQT
                     TKNNARTRKGKRKTVANKKKATK"
     gene            1441..1869
                     /gene="rpsK"
                     /locus_tag="VFU15_11565"
     CDS             1441..1869
                     /gene="rpsK"
                     /locus_tag="VFU15_11565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013186928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEU4718468.1"
                     /translation="MAKQQQQTSGKTAATSGKAAARKRTVKVDAVGEAHINATFNNII
                     ITLTNTSGQTISWSSAGKMGFRGSKKNTPYAAQTAASDAAKTAFDAGLRKVKVYVKGP
                     GAGRESAIRTLNQAGIEIVEIIDITPIPHNGCRPPKRRRV"
     gene            1905..2507
                     /gene="rpsD"
                     /locus_tag="VFU15_11570"
     CDS             1905..2507
                     /gene="rpsD"
                     /locus_tag="VFU15_11570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018279609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEU4718469.1"
                     /translation="MARYIGPKSKIARKFREPIFGADKALEKKAYPPGQHGLASKRAK
                     KSEYATQLAEKQKVKYTYGLLERQFALVFDRASRAKGITGEVLLQLIEARLDNVVYRM
                     GIAPTRSAARQLVSHRHITVNGEIVNIPSYTLKPGDVVAVREKSKSMEAISNSVGSGS
                     GYAWIEFNPTALEGKFVNFPERTQIPENIKEQLIVELYSK"
     gene            2585..3577
                     /locus_tag="VFU15_11575"
     CDS             2585..3577
                     /locus_tag="VFU15_11575"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010251770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEU4718470.1"
                     /translation="MGILAFQKPDKVIMIQSSETDGLFEFRPLEPGYGITIGNALRRI
                     LLSSLEGYAITTLRIEGIEHEFSTIKGVMEDVTEIVLNLKQVRFKRQIDTTDSEKVVV
                     NITGKTQLTAGDIGKFTSGFQVLNPDFVICNMEANVKLKIELTIEKGRGYVPADENKP
                     VNAPIGLIPVDAIYTPIRNVKYSVENYRVEQKTDYEKLVIEIVTDGSVHPKEALKEAA
                     KILIHHFMLFSDDKITLDTVEKQSSEEFDEDSLHMRQLLKTKLVDMDLSVRALNCLKA
                     ADVETLGDLVSFNKNDLLKFRNFGKKSLTELEELVASKGLQFGMNVTKYKLDRD"
     gene            3619..4143
                     /gene="rplQ"
                     /locus_tag="VFU15_11580"
     CDS             3619..4143
                     /gene="rplQ"
                     /locus_tag="VFU15_11580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014203388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEU4718471.1"
                     /translation="MRHGNKDNHLGRKDGHRRALLANLAASLIMHKRINTTVAKAKAL
                     RMYVEPLITRSKDDTQHNRRTVFSELGNKAAVTELFKEVGPKVANRPGGYTRIIRYSL
                     SISPKPAARRRGDSADMCLIELVDFNELMLNAKSEGKKAKTTRRGRGKSKAKATDTTA
                     ENTSNESNAEEKSE"
     gene            4227..5378
                     /gene="carA"
                     /locus_tag="VFU15_11585"
     CDS             4227..5378
                     /gene="carA"
                     /locus_tag="VFU15_11585"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013927409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEU4718472.1"
                     /translation="MEEKETRSYRLKYNALPPAVLLLEDGTVFYGKAAGHIGITTGEI
                     CFNTGMTGYQEIFTDPSYYGQILVAANVHIGNYGIRKEEVESDSIKIRGLVCKKFSEF
                     YSRPGAEKSIRDYFLEEKIVGISEVDTRAIVRHIRDKGAMNAIISSSEMDIEVLKKKL
                     SEVPSMAGLELSSKVSTKEAYTSGDPGSKYKVALLDVGVKKNIVRCLVERGCHVKVFP
                     MKASAAEMMAWSPDGFMISNGPGDPSVMHDEVETVKQLIDSGTPVFGICLGHQLIAQA
                     FGLSTYKMHNGHRGINHPVKNLLTGRSEITSQNHGFAVNAEEVSKQEDIEVTHVNLND
                     NTIEGLRHKVKKVFCVQYHPEAAPGPHDSRYLFDDFVKLMASVKKGAAV"
     gene            5456..6745
                     /gene="eno"
                     /locus_tag="VFU15_11590"
     CDS             5456..6745
                     /gene="eno"
                     /locus_tag="VFU15_11590"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014203390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEU4718473.1"
                     /translation="MSFIGQIQARQILDSRGNPTIEVDVVTTTGTLGRAAVPSGASTG
                     SHEAVELRDGDKKVYMGKGVLKAVSNVNTIIKEELNGTYIFDQAAIDAKMIALDGTPN
                     KANLGANAILGVSLACAKAAAEEARQPLYRYVGGVNAMLLPIPMMNILNGGAHADNGI
                     DFQEFMIMPVGADTFGDALRMGTEVFHNLKKVLKDKGYATNVGDEGGFAPNIKNNVEA
                     IEYVLKAIEAAGYKPGDDIYVAMDAATSEFYDAATKTYTFKKSDGKKLSSEEMVGYWA
                     EWIKKYPILSIEDGLAEDDWDGWEKLTAEIGDKVQLVGDDLFVTNTDRLADGIGRGVA
                     NSILIKVNQIGTLTETIDAVTMAHTNSYTSVMSHRSGETEDTTIADLAVALGCGQIKT
                     GSASRSDRIAKYNQLLRIEEQLGGSARFLGKQFRYIP"
BASE COUNT         1928 a         1611 c         1871 g         1402 t
ORIGIN      
        1 gaaggaaggc gatgtggttt cggtggattg cggcgtggtg atgaacggct tctacggcga
       61 ttcggctttt acttttccgg ttggtgaagt aaatcccgag gtaatgcgcc tgcttcgcgt
      121 aacgcgtgaa tgcctttaca agggtattga aaaggcggtc gcgggaggaa gaattggtga
      181 catcagcgaa gctgtgcagc agcatgctga gaagaacggc ttttccgtgg tgcgtgagct
      241 ggtgggacat ggtgtcggaa agcaccttca tgaggcgcct gaagtgccga atttcggaag
      301 gcgcggaaac ggaccgaagc tcgcggaggg tttggtgatc gcgattgagc cgatgatcaa
      361 cctcggcaag aaggaggtca ggcaggagcg tgacggctgg acaataacca ccgcggatgg
      421 attgccttca gctcattatg agcatacaat tgcgatccac aaagggaaag cggacatttt
      481 gtcttcgttc gacgagatcg aacaggtact gaaagaaaaa cagatagtaa tctgaaaaaa
      541 taaatggcga aaacaccatc aatagagcag gacggaacca ttatcgaggc attatccaac
      601 gcgatgtttc gtgtggagct tgaaaatggt cacgtgatca cggctcatat ttccggtaag
      661 atgcggatgc attacatcaa gatccttccc ggggataaag tgaaagtgga aatttcgcct
      721 tatgatctta cgaaaggccg gatttcatat cgctataaat aagatcgctt tgctgttttg
      781 cctgtaaaag caaaacaaca gaacaggata agacagtaaa aacagaaaaa cagagaacaa
      841 tgaaagtcag aacatcaata aagaagcgca gcgcggactg catcgttgtt cgccgcaaag
      901 gacgtcttta tgtgatcaac aaaaagaatc ccaagttcaa gcagcgccag aaaaactgat
      961 cgaacggaat taaaacgaac caaattagaa ccaaatagtc aatggcaagg attgctggta
     1021 ttgatttacc gaaaaacaag cgtggcgaag tcggcctgac ctatatcttc ggaataggcc
     1081 gtaacacggc gcgcagaatt ctcaccgaag ccggtgttga ttttgacacg aaagtccagg
     1141 actggaatga cgaccagctg aacaagatcc gcagcgttat cagcgaaaaa atcaaagttg
     1201 agggcgccct gcgttcagag gtgcagctca atatcaagcg cctgatcgac gtcggttctt
     1261 accgcggcat gcgtcaccgt aatggtcttc ccgttcgcgg ccagaccacc aagaacaacg
     1321 ccaggacgcg taagggaaaa cgtaaaaccg ttgcaaacaa gaaaaaggcg actaaataat
     1381 aaatcatacg cttccggcgg attgtttaac gtcctgcagg cagcgtaaat gataaaagac
     1441 atggccaaac aacaacaaca gacaagcggt aagacagcag ccacttcagg aaaagcggca
     1501 gcgcgtaaac gcaccgtaaa agttgatgca gtaggagaag cgcatatcaa cgctacgttc
     1561 aacaacatca tcattacgct gacgaacact tccggacaaa ccatttcatg gtcgagcgcc
     1621 gggaagatgg gtttccgcgg atccaagaaa aatacgcctt atgcagccca gaccgctgct
     1681 tctgatgctg cgaaaacagc attcgacgcc ggactgcgta aagtgaaggt gtacgtcaaa
     1741 ggaccgggag cgggacgtga atcggctatc cgtacgctta accaggcagg tatcgagatc
     1801 gttgagatca tcgacattac gcctattccg cacaacggct gccgtcctcc gaaacgccgt
     1861 cgtgtataac ccagatctaa aaacaattca actgataaag aaagatggct agatacatcg
     1921 gaccaaagtc aaaaattgcc cgcaaattcc gggagccgat tttcggagca gataaagcgc
     1981 ttgaaaagaa agcgtatcct ccgggacagc acggacttgc ctccaagcgt gcgaagaaat
     2041 ctgaatatgc aacccagctt gctgaaaagc agaaggtgaa atatacctat ggtctcctcg
     2101 agcgccagtt cgcacttgta tttgacaggg cgtcacgtgc aaaaggcatt acgggtgaag
     2161 tgcttctcca gcttattgaa gcacggctgg acaatgttgt ttatcgcatg ggaattgcgc
     2221 ccacgcgttc tgcagcccgc cagcttgttt cccaccgtca tatcaccgtc aacggggaga
     2281 ttgtcaatat cccctcctac acgctgaagc cgggagacgt ggttgctgtg cgtgaaaaat
     2341 ccaagtcgat ggaagccatt tccaattccg tcggaagcgg gtccgggtat gcatggatcg
     2401 aattcaatcc tactgcgctc gaagggaaat tcgtgaactt ccccgaacgc acgcagatcc
     2461 ctgaaaacat caaggaacag ctcatcgtcg aattgtattc gaaataagga tgaagacggc
     2521 tatcggtggc ctgcaattca ccggcgaaaa cataaatgtt ttttaacgaa caagcagaat
     2581 caaaatgggt atcctcgcat tccagaaacc tgataaagta ataatgatcc agtcgagcga
     2641 aacggatggt cttttcgaat tccgtccgct tgagcctggt tacggcatca cgatcggcaa
     2701 cgcgctgcgc cgtattcttc tttcttcgct tgaaggctat gcgatcacga ctcttcgcat
     2761 tgaaggcatc gagcacgaat tttcgacgat caagggtgtg atggaagatg tgacggagat
     2821 cgtcctgaac ctgaagcagg ttcgtttcaa gcgccagatc gataccaccg acagcgaaaa
     2881 agttgtcgtc aacattacgg gcaaaacgca gctgactgca ggtgatatcg gaaaattcac
     2941 ctccggtttc caggtgctca atcccgattt cgtgatctgc aacatggaag cgaacgtgaa
     3001 gctcaagatc gagctgacga tagaaaaagg ccgcggttat gttccggctg acgagaacaa
     3061 gcccgtgaat gcaccgatcg gtcttatccc ggtggatgct atttacacac cgatccgtaa
     3121 cgtaaaatat tccgttgaaa actatcgtgt agaacaaaaa acggactatg agaaactcgt
     3181 gatcgagatc gttaccgacg gttctgttca cccgaaagaa gcgcttaaag aagcagcgaa
     3241 gatcctgatt caccacttca tgctcttctc tgacgacaag atcacactgg atacggttga
     3301 aaagcaatcc agcgaggagt ttgacgaaga ttcacttcac atgcgccagc tgctcaagac
     3361 caagctcgtt gacatggacc tttccgttcg cgcgctcaat tgcctcaaag cggctgacgt
     3421 tgaaacactt ggcgacctcg tttcttttaa caagaatgac cttctgaaat tccgcaactt
     3481 cggcaagaaa tcgctgacgg aacttgaaga gcttgttgca agcaaaggac tccagttcgg
     3541 aatgaacgtg accaaataca agcttgatcg cgactaatcg actatcaccg cgctgagcgg
     3601 taaatacgta agactaaaat gagacacgga aataaagaca atcatctcgg gcgtaaagac
     3661 ggccaccgcc gtgcgctgct ggccaacctg gcggcgtcgc tcatcatgca caagcgcatc
     3721 aatacgacgg tagcaaaagc gaaagcgctg cgcatgtacg ttgaaccgct gatcacgcgt
     3781 tcaaaagacg acacacagca caaccgcagg accgtttttt ccgagctggg aaataaagca
     3841 gcggtaacag agcttttcaa ggaggtcggc ccgaaagtgg cgaaccgtcc gggcggttat
     3901 acgcggatta tccgttacag ccttagcatc agtcccaaac cggcggcgcg acgcagaggc
     3961 gacagcgcag acatgtgcct gatcgagctg gtggacttca acgaactcat gctgaatgcg
     4021 aagagcgaag gcaagaaggc gaagaccacg cgccgcggcc gtggaaaatc caaagcaaaa
     4081 gcaacggata caacggcaga gaatacgtca aacgaatcca acgctgagga gaaaagtgaa
     4141 taatttgtca gcaagttgat ttcagtacca aaaagggata aggtaatttt gccgtatccc
     4201 tttttctttt ctaaataaat ccgattgtgg aagagaaaga aaccagatcc taccgactta
     4261 aatacaacgc gcttccgccc gcagttctct tactggagga cggaactgtt ttttacggga
     4321 aagcagcggg ccacattggc attactaccg gcgaaatatg tttcaatacc gggatgaccg
     4381 gttaccagga gatcttcacg gatccttcct attacggcca gatccttgtg gcggcgaacg
     4441 tgcacattgg aaattacggc atcaggaagg aagaggttga gtctgactcg atcaagatcc
     4501 gggggcttgt gtgcaaaaag ttcagcgaat tttactcccg gcccggggcg gaaaaatcca
     4561 tcagggacta ttttctggaa gagaaaatag tgggcatcag cgaagtggat acgcgggcga
     4621 tcgttcgtca cattcgtgat aagggcgcga tgaatgcgat catttcgagc tctgaaatgg
     4681 atattgaagt gctgaagaaa aagctttcgg aggtgccttc gatggccggc cttgagcttt
     4741 cctcgaaagt atccacgaaa gaagcgtaca cctccggcga tcccggatcg aaatacaaag
     4801 tggcgctgct tgatgtgggt gtgaagaaga acatcgtccg ctgcctggtc gagcgtggct
     4861 gtcacgtgaa agttttcccg atgaaggcat ctgctgcgga aatgatggcc tggagcccgg
     4921 atggctttat gatctcgaac ggccccggcg atccttcggt catgcacgat gaagtggaaa
     4981 ccgtgaagca gctcatcgac agcggaacac cggtattcgg tatttgtctc gggcaccagc
     5041 tgatcgcgca ggcgttcggg ttaagcacgt acaaaatgca taatggtcac cgcggcatca
     5101 atcacccggt gaaaaacctg ctgaccggca ggagcgagat cacttcccag aaccatggct
     5161 ttgcagtgaa cgccgaagaa gtttcaaagc aggaagatat cgaggttacg cacgtgaacc
     5221 tgaatgataa tacgatcgaa ggtctgcggc acaaagtgaa aaaagttttc tgcgtgcagt
     5281 atcacccgga agcagcgccc ggcccgcatg attcgcggta cctgttcgat gattttgtga
     5341 agctgatggc cagcgtcaaa aaaggcgctg cggtttagcc ttctgccatt tcctgatccg
     5401 ttcctgtttt catcggcagg aaagaaaaat aacctttaat caatcaccat agcttatgtc
     5461 atttatcggc cagatccagg cgagacagat tctcgattcc cgtggaaatc caactattga
     5521 agtggacgtc gttacgacga ccggaacgct tggccgtgcg gctgtgcctt cgggcgcttc
     5581 cacgggcagc cacgaagcgg ttgagctgcg tgacggcgac aaaaaagtgt acatgggaaa
     5641 aggcgtgctg aaagcggtga gcaatgtgaa caccatcatc aaggaagagc tgaacggaac
     5701 gtacattttc gaccaggcgg caattgatgc aaagatgatc gcgctggacg gaacaccgaa
     5761 caaagcgaac cttggtgcaa atgcgatcct cggtgtttcg ctggcgtgcg caaaagcggc
     5821 agcggaagaa gctcgccagc cgttgtatcg ttacgtgggc ggcgtgaatg cgatgctgct
     5881 gccgatcccg atgatgaaca tcctgaacgg aggagcgcat gcggataacg ggatcgactt
     5941 ccaggaattc atgatcatgc cggtgggagc ggacactttc ggcgatgcgc tgcgcatggg
     6001 gacggaagtt tttcataacc tgaagaaggt tttgaaagac aagggctatg cgacaaacgt
     6061 cggtgatgaa ggaggttttg cgccgaatat caaaaacaac gtggaagcga tcgaatacgt
     6121 gctgaaagcg atcgaagcgg cggggtacaa gccgggagat gatatctatg ttgcgatgga
     6181 cgcggccact tcggaattct acgatgcggc gacgaaaaca tatacgttca aaaagtcgga
     6241 cggaaaaaag ctgagcagcg aagagatggt gggttactgg gcggagtgga tcaaaaagta
     6301 cccgatcctt tcgatcgagg atgggcttgc ggaagacgac tgggacggct gggaaaaact
     6361 gaccgcggag atcggggata aagttcagct ggtgggcgat gatcttttcg ttacgaacac
     6421 ggaccgtctc gcggacggca tcggcagggg cgtggcgaat tcgattctta tcaaagtaaa
     6481 ccagatcggg acgctgacag aaacgatcga cgcggtgacg atggcgcata cgaattccta
     6541 cacgagcgtg atgagccacc gttccgggga aacggaggat acgacaattg ccgacctggc
     6601 ggtggcgctg ggttgcgggc agatcaaaac gggttcggct tcgcgttcgg acaggatcgc
     6661 gaaatacaac cagctgctgc ggatcgaaga gcagcttggc ggatcggcgc gtttcctcgg
     6721 gaagcaattc cgctacatcc cgtgaatgcc gccgggcttt gatgaggtgg agtcctgacc
     6781 gcatagccct gattgaagcg gaaccgcggc cg
//
LOCUS       DASTSF010000405        18423 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_456202,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000405 DASTSF010000000
VERSION     DASTSF010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 18423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18423
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_456202"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(262..1767)
                     /locus_tag="VFU15_11595"
     CDS             complement(262..1767)
                     /locus_tag="VFU15_11595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718474.1"
                     /translation="MKKYFPVMILLILSLCGGKEIAAQNELADIMKSDSLTLPAISIY
                     PDSIRTAMCIAGEYPEVLVKMENLQGASQSAFHAILAPYSQEEQQKIWDLSRYPDLLQ
                     RIGSGKENAEKIAADYPAEIHDRIIYVAANRKDVAQKIVKLQQSADSSFNGIIASYPA
                     EAQDAFRKLLLYPETVKLLTDNMRTTVMIGDLYRRNPKQLGHQLDSIQMIEAQQRTQE
                     AKDWQDGLAKDTAAQREMKEAVNEYAQQNPTGSTDLTSPTTTQVIIVQQYVYPYPYWY
                     GYPVWYDYPCWYPTPWWYDCGYYCGPQGCVWIGYGYPSPFFFHWYFWYYPHHYHYHHF
                     SDYCVHHYEHHRRSTSPSDGEIGGWMQAQHPYVSPDFFVDDGKRADRLKSLGKEEMDR
                     QQYNVAHPAQQVSRDAYIRQHSGNYPGVQVAPPPVPAKPGDANSGQKPYTPPPAKPQR
                     NPVPTNPNQNTRPTYPNQPRPQQNPPPRNNPPAPKPNPPQKSPPPSPKKPR"
     gene            2153..2329
                     /locus_tag="VFU15_11600"
     CDS             2153..2329
                     /locus_tag="VFU15_11600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718475.1"
                     /translation="MIVPITEEDRRRNPSATKGKDYKLVSVTSGRVLGYGSIEELKKR
                     ERQVQYFKHKKTNP"
     gene            2352..2819
                     /gene="bfr"
                     /locus_tag="VFU15_11605"
     CDS             2352..2819
                     /gene="bfr"
                     /locus_tag="VFU15_11605"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006365810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HEU4718476.1"
                     /translation="MKGNKKIISRLNELLADELTAIGQYVVHAEICENLGYTKLHDVI
                     KRRAIDEMGHAEKLIARILFLEGMPEIHGMKKISIGNDVAKQFKSDRDAELEAVKMYN
                     ESIGLVSEQDDNGTRDLLVSILKDEERHLDWLETQNSLVENLGLQNYLLSQAG"
     gene            3092..6757
                     /locus_tag="VFU15_11610"
     CDS             3092..6757
                     /locus_tag="VFU15_11610"
                     /inference="COORDINATES: protein motif:HMM:NF012999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEU4718477.1"
                     /translation="MFAFLLAMAGLKAQVTCTDLNNYVDSKNTGPTGYYTLQNGQEEM
                     AAQTYHYSVAGKVNSVRVYGNYPGVVGGVPLRVSIANVDANGRPTTTIQSVNVTWWWF
                     DNFSSYMTVNFPGGGVTVNNNYAITVELRNASPWGSSFQLQYTGDGEGLGQDLASLAG
                     TSTGHNWTSAMTNFSKDGDFYLVPRMTHYITPAFSVSSNCASVGTPVTFSNQSIMTTD
                     SMFNIIGLSNYSGSNYFYTWNFGDASPVSHAANPTHTYATAGSYTVTLTCMIDGWFTD
                     GSATKTDVISVGLGSNATATSVSCNGGSNGTITINATGGSSPYSYSINATGFQPGNMF
                     SGLGAGTYTTYVQDNVGCISTGSVTITQPSAIVISNVSSTNSSCGNADGALLISASGG
                     TGTLQYQLNSNPYQSSNSFTGLGSDFYTVTVKDANGCTTSTNAAVNDQGAPSLTLVSM
                     TNISCNSGSNGTIVLNGTGGSGTLQYSTNGGNTWQTNGSFLNLPAGDYLAMVKDASGC
                     SSAMEITLHEPPAISFQVSSTPVGCHGDNDGTVTATAATGGTGTYSYSLNNVNYQSGS
                     MFTGVTAGTYTVYVRDIAGCVATSSVTVTEPTALTASTSVINAGCNGSYDGGITATAT
                     GGTAPYIYSLNGEEWQSSGTFDELESGTYMITIRDANGCQVLVNATITQPTLVTASIT
                     TGASTCGNANGTLLAVGSGGTGSGYTYSIDGTNFNSSGSFSSLTSGTYNVIVRDGNGC
                     QNVLTTFINDANGPSITSVSSTNVACHDGTDGTITVNSVSGGTGTLQYSVGTTWQLSN
                     SFTGLDAGNYNVLVQDANGCVGQTSVTLTQPNAIVVTTSSTDVTCHGSNNGSVIVNAA
                     GGAGTLAYAIDNEAPFQSSNVFNNLYAGTYYTIVRDAAGCLGSAYFTVNEPAAITMNV
                     SQLNVACYGGNDAAIYIMAMGGTGTLTYSLNGINYQSASSFLNLTAGNYTVYVKDANG
                     CVETQMVIITQPSPLVMNYTVSDVVCSGGNDGVIDLSISGGVHPYYFDWSNNQTTEDI
                     FNLSAGTYSVTLTDGNGCVYSQAFTVSQPANALIVNGVITDATSQSTNDGGVSLTVTG
                     GTGPYTYLWSNGATTQDISNVAPGVYSVIVTDANGCTSSGIFLVSVVSGVVNHSIDGG
                     VKLYPNPANDNFTIDAGNSVIDRVEIYDMLGQVVFANEPKTPKTQISTDGLSQGSYFV
                     QMYIGGQKVTMRLQVSK"
     gene            7200..8261
                     /locus_tag="VFU15_11615"
     CDS             7200..8261
                     /locus_tag="VFU15_11615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU4718478.1"
                     /translation="MSNTILLIEDNPEMRENTKELLELAGYSVTEAINGKDGLEKAHN
                     QKPDLILCDVMMPELDGYGVLRAMNNVPHLSGVPFVFLTAKSEKSDFRLGMDLGADDY
                     LTKPFEGDELLRVVEARIRRSNLLKKKYAQNLEGLDEFMAEAQSIGELVNLSSGKTVK
                     KLRAKDMVYMEGDTANYLFFVVSGRIKTSKTNEWGKELLLDIYSAGDFFGYQAMLEEG
                     AYQQSAQAIENSEVALIPKKDFLALLYSSREVSLQFIRMMSNNMNTAETKLLKLAYDS
                     ARKRVAEALLLIAKKYEQKSSGEVSFPGLRENISGIAGISPESVSRNLTDFREEGLIE
                     TDNGEIRIPDIKKLEKLRF"
     gene            complement(8686..10029)
                     /locus_tag="VFU15_11620"
     CDS             complement(8686..10029)
                     /locus_tag="VFU15_11620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin esterase family protein"
                     /protein_id="HEU4718479.1"
                     /translation="MRTRGGNKWNEEMPGKQEAADFICECAHGLENASGLDALMEAIG
                     DAQYVLLGEASHGTHEYYTWRTHITRRLIAEKNFSFVAVEGDWPDCYRLNRYVKGYAD
                     AGNSAKEVLQEFNRWPTWMWANWETVALAEWLHKFNRNQAPGRKAGFYGLDVYSLWES
                     IEAIIKYLERTDRVAAEAARKAVRCFDSFGHDEGRSYAQAAALIPASCEEEVTDLLAT
                     VRRGMKHYNTDPEGALNAEQNAVVAVHAEHYYRTMMKGGISSWNVRDHHMTETLERLV
                     NFHGTGSKAIVWEHNTHIGDARATAMSAGGMVNVGQLLNEKYGSRKVFRVGFGSYSGS
                     VIAGKEWGEKMERMNVPRAMEGSWEELLHRTGMTVFYLLTESLKKKHFFREALGHRAI
                     GVVYHPEYEQHGNYVPSIIPLRYEAFVFIDQTKALHPLHVPASESQVPETYPWGI"
     gene            10173..10826
                     /locus_tag="VFU15_11625"
     CDS             10173..10826
                     /locus_tag="VFU15_11625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007653563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HEU4718480.1"
                     /translation="MRTLLFSNRAEAGLRLAGALEKFRGTDSVILAIPRGGVEVAKVV
                     AETLSLPLDIALSKKIGHPANSEYAIGSVSPDTVVLDEHADVSQEYILRETKKIRELL
                     QKRYDLYMGARKPVALKGRTAIVVDDGIATGNTLLATIRTLRKKEPRSIVVAVPVAPS
                     RGTERIKTEADEFICLYTPADFPGVSAFYADFAQVSDEEVVQLLSENAPHGSEKEIT"
     gene            10823..11455
                     /locus_tag="VFU15_11630"
     CDS             10823..11455
                     /locus_tag="VFU15_11630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009579913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU4718481.1"
                     /translation="MNAEEIFIPIGDITLPALLDVPGKAGGVVIFSHGSGSSRLSTRN
                     RFVAEILHRKNFATLLFDLLTEEEDRIYENRFDIDLLTQRLISVTRWIKKIPGTKDLR
                     TGYFGASTGSASALKAASHLGNEIGAVVSRGGRPDLAGDIRGVTAPTLFIVGGLDTSV
                     ILLNREASAHLTCVHEMRIIEGASHLFEEKGKLSEVAKEAAEWYEKYLTA"
     gene            complement(11477..12304)
                     /locus_tag="VFU15_11635"
     CDS             complement(11477..12304)
                     /locus_tag="VFU15_11635"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEU4718482.1"
                     /translation="MLRRIVCPIDFSPAARNAVQYAAKLAQLSGAGLELVYFQYHSSR
                     ALMAVAGRKKADAEAHVMKVARELENLAAELNKMFSISCGTGVETGEETLTEAIAGKA
                     GPGDLIVAGTNGADDLFQDFFGSNAYNLAVHAGCPVLVVPEQVEYRTLTEIVFAWDYD
                     PESRPLFVRLKELAGMLGAQITFLHVSRHISDVSKDIFNALKEKVGEVFSEGEAKFER
                     IIAPHPQDSIDEYMRGKESAVLALAVQKGKMLREILREKHGSEEELTAAYPVLVMHA"
     gene            complement(12316..12651)
                     /locus_tag="VFU15_11640"
     CDS             complement(12316..12651)
                     /locus_tag="VFU15_11640"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEU4718483.1"
                     /translation="MLDGSEQSFVVTENGNVTGILTQKELVHGLSQDGKHTRVADVMR
                     RDFLSLAPQMHLEDAMQKMLAAGQYLCPVMENGRLAGVIDLQNINEFILINGAIRKKE
                     ITGSGVSDE"
     gene            complement(12618..13436)
                     /locus_tag="VFU15_11645"
     CDS             complement(12618..13436)
                     /locus_tag="VFU15_11645"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEU4718484.1"
                     /translation="MNFLRGSLYIGAIRETKIFVHWTFFLLIAWVFISAYREHGDLGQ
                     GIISVLFVLVFFACITLHEFGHITVARRYGCYSKKITLLPIGGVASMDKIPEKPLQEM
                     LMALAGPWVNFVIAAFIWLFLWISKGLPSSAVITTLTRDNFLYVLMTANLVVAFFNLI
                     PAFPMDGGRVLRAILAMRFPRERATKIAANVAQVIAVVFVITGLFVNVWLTFIGVFVF
                     LGATSEATAEMTKSLLSHYHVRNVLMHRFTCLSPEEDLESAARKCSMAASKALS"
     gene            complement(13514..13960)
                     /locus_tag="VFU15_11650"
     CDS             complement(13514..13960)
                     /locus_tag="VFU15_11650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEU4718485.1"
                     /translation="MSLIKRNNGNAPSTRTFFSDFFDADRFFREPFLSVTDGEWVPAV
                     NVVENEKDFSIELAAPGFKKSDFKVNVENGILNISAEKKTEKEEKEKNYTRREFSYNS
                     FSRSFSLPENSNEENVAAKYDDGVLKLTLAKKVPGSPKAKKEVEVA"
     gene            complement(14152..17784)
                     /locus_tag="VFU15_11655"
     CDS             complement(14152..17784)
                     /locus_tag="VFU15_11655"
                     /inference="COORDINATES: protein motif:HMM:NF040138.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HEU4718486.1"
                     /translation="MSTARIFPFIIFTFAISPALFAGNNSAPVQKLDAPLLRFEENKN
                     QFDPRVVYEADLTRSGKVFFEKNAFTYLFWNADEIEQLHHPSPVNGVISPDQDVTVHY
                     HCFRAEFLGANSDPFISAGLPAPYYKNYFLGNDPAKWAPEVHLYDEMDYNELYPSVGV
                     HLYSRDGNLEYDFKVDEDGDPSVIRINYAGADKVSLNDGNLVIHTSLGDVTEQRPYAY
                     QVIDGRETAVRCRYVLHGTVASFEFPDGYDHAWPLVIDPTLVCSTFSGSTSDNWGYTA
                     TYDAQGDIYSGGIGTAIGYPTTAGAFQTTFGGGGSGGNAYPFDITLTKYNPSGTALLF
                     STYLGGSDNEQPQSIVVDSNDELCVIGRTYSSNFPVTAGAYDVSYNGNADIIVTKFNS
                     TGTALVGSTFVGGSGDDGVNITANFYTWMSLKYNYGDDGRSDIITDNAGNCYIASSTQ
                     SANFPVTGGAYQSSFGGGTQDGCVFKLNSNLTSMTWSTFLGGSGDDAVYSIQLDGTNS
                     VYVSGGTSSSNFPTTPGTLHTTFQGGTADGFISHLDPSGVAMLQSTFIGTNGYDQAYF
                     IQLDNSSNVYVYGQTAGAYPVSAGVYSNPNSGQFIHKMNASLSSTFYSTVFGSGTGTP
                     NISPSAFLVDTCENVYAAGWGGQCIPYGSTGTTVGLPVTSNAFQSTTDGCDFYFFVLK
                     KNAISLWYATYFGGNLGSDEHVDGGTSRFDKRGVIYQSVCAGCGGSQNFPTTPGAWST
                     TNNSFNCNNAVIKMDFQLVNLSAVAVASPSDSICLGTTMSFANNSIGAQDYEWNFGDG
                     SPADTSTAPSHQYTTPGSYTVTLIAIDSLGCNTSDTTQIYVTVLPVPVVDLGPDTTFC
                     GTPNLTLDAGNPGAQYTWNTGATSQTINVNSAGTYWVTAGNGVCSDKDTITVSVFTPP
                     DLGGDTTLCEGNPVTLDAGNPGSTYAWSTGAATQTISVNSSGTYSVTVTSGTCQLSDS
                     IIVNFVAVPQVDLGPDLSLCPGQTATLNAGNPGDTYLWSNNSIEDSITVSAGGTYYVT
                     VWNSGCSASDSVNVQAIAPFDLGPDVNLCDNAGIHLVSENLGGASYLWSTGDTTFSIE
                     VNEAGTYWVNISTGNCMLTDTIDVIGGFGPSTVYVPNCFTPNHDGKNDIFYAEGVDII
                     SFHMMIFDRWGEMIFESYDMGQGWDGTYKGDKVPNDVYVVVIDFTTQCSGENTVRKIS
                     HVSVYK"
     gene            complement(18186..>18423)
                     /locus_tag="VFU15_11660"
     CDS             complement(18186..>18423)
                     /locus_tag="VFU15_11660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HEU4718487.1"
                     /translation="VKRIAHKLISIKHKEVELLEPYDDNEFNTISVNFEQFLERIRNE
                     LCVPKNMLRLSMNVKEQDIKMIYIPWTLKLARQK"
BASE COUNT         4478 a         4956 c         4578 g         4411 t
ORIGIN      
        1 gcaggatgcg gtgataaaag ttttcgtgat gctccgcaaa tatgtttctg aaggcagcct
       61 tgaaaaaatt gccgacgtgc ttcccgcaga aatgaaatcg cttctctacc cggcgatcac
      121 gttcccggca ttgtaaaaaa catgtgcgaa aaacggatgc tcattaccgg gcatccgttt
      181 tttgccgggc actaacaggg ttgcaaaaaa agaaaccggg taattcaacc cggttttgtt
      241 ttttcttctg acgcttacac tttatctcgg ctttttcgga gacggcggag gagatttctg
      301 cggaggattc ggtttcggcg ccggaggatt attgcgcggc ggcggattct gctgcggcct
      361 cggctggttg ggataagtcg ggcgtgtatt ctggttcggg ttggtcggaa caggattccg
      421 ctgcggtttt gccgggggcg gtgtgtatgg cttttgtcca ctgttggcat cgccgggttt
      481 tgcaggtacg ggaggaggag caacctgtac acccggataa tttccgctgt gctgcctgat
      541 gtatgcatcg cgtgacacct gctgtgcggg atgcgctacg ttgtattgct ggcggtccat
      601 ttcctctttt ccaagcgatt tcaacctgtc cgctcttttt ccatcgtcca cgaaaaaatc
      661 cggtgaaacg tagggatgct gcgcctgcat ccatcctcct atttctccat cggaaggcga
      721 tgtggaacgg cggtgatgct cgtaatgatg cacgcaataa tctgaaaaat gatggtaatg
      781 ataatgatgc ggataatacc agaaatacca atgaaaaaag aaaggcgaag gatacccgta
      841 accgatccag acgcacccct gcggtccgca gtaatacccg caatcatacc accacggcgt
      901 cgggtaccag cacgggtaat cataccacac cgggtagccg taccaatacg gatacgggta
      961 aacatattgc tgtacgatga tcacctgtgt tgtcgttggc gatgtgaggt ccgtgcttcc
     1021 cgtggggttc tgctgcgcat attcattcac cgcttccttc atctcccgct gcgctgctgt
     1081 gtctttcgca agtccgtcct gccagtcttt cgcttcctgc gtgcgctgct gcgcttcgat
     1141 catctggatg gaatcgagct ggtgcccgag ctgcttcggg tttctccggt acaggtcgcc
     1201 gatcattacg gtcgtccgca tattgtcggt aagcagcttc actgtttcag ggtacagcag
     1261 gagtttccgg aatgcatcct gcgcttccgc aggataagaa gcgatgatcc cgttgaagga
     1321 agaatcagcg ctttgctgca gcttcacgat cttctgcgcg acatctttcc ggttcgcggc
     1381 aacataaata atgcggtcgt gtatctcagc cgggtaatcc gcagcgatct tttccgcatt
     1441 ttcctttccg ctgccgatgc gctgcaacag gtcaggatac cggctgaggt cccagatctt
     1501 ctgctgctct tcctggctgt acggcgcaag gatggcatga aacgcagatt gggaagcgcc
     1561 ctgtaagttt tccattttca ccagcacttc cgggtattca ccggcaatgc acatcgcggt
     1621 ccggatgctg tccgggtaaa tggaaatagc aggaagtgtg aggctgtcgc tcttcatgat
     1681 gtctgccagc tcattttgcg cagctatttc ttttcctccg cagagagaaa ggatcagcag
     1741 gatcattaca ggaaaatatt ttttcatctc ggttcttttt cgtggatttc ttttgaccag
     1801 gtttcaaaag tatccgctat tccttccatt caccctgaca gtggtcacaa gccggggtga
     1861 cactgaccat ccaggcatcg ccgcatctgc attaatgatc tatgtcttcc tgccggttga
     1921 tgtgaatcac atcgcatgat tctcccggcg gatatttttg cgacaatcaa aagaaacaaa
     1981 atgggaactg taccgatgtt tacgctgatc gcgctggcct gtgtccagct cctcatgaat
     2041 tctttaagga acgctcggct tctgaaatca cgggggacaa agaattcccg caggattcct
     2101 gcggagctga cggtccgcac cgaaaagatc cgttcctgaa aaaacctgtc gcatgatcgt
     2161 tcccattaca gaagaagacc gcaggcgcaa tccttccgcc acaaaaggaa aggattataa
     2221 gcttgtttca gtaaccagtg gtcgcgttct cggctacgga agcattgaag aactgaaaaa
     2281 gagggaacgg caggtacagt atttcaagca caaaaaaacc aacccgtaat tcatattcaa
     2341 aattcataga catgaaaggc aacaaaaaaa ttatttcccg cctgaacgag ctcctcgccg
     2401 acgagctgac agcgatcggt cagtatgtcg tgcatgcgga gatctgcgag aacctcggct
     2461 acacgaagct gcacgatgtg atcaaaagac gtgcgatcga tgaaatggga catgcggaaa
     2521 aactcatcgc gcgcatcctt ttcctcgaag gaatgcccga gatccacggc atgaagaaaa
     2581 tttccatcgg caacgatgtc gcgaagcaat tcaaaagcga tcgcgatgca gaactggaag
     2641 ccgttaaaat gtataacgaa agcatcgggc ttgtttcgga acaggacgac aacggaacac
     2701 gcgacctcct ggtttccata ctcaaagatg aagagcgtca cctcgactgg ctcgaaacac
     2761 agaacagcct ggtcgaaaat ctcggcctgc agaattatct tctttcacag gccggctgaa
     2821 cagcattcca tttttctcgg gaaagaacgg gcaggtaaca aaagttgcct gctttgtttt
     2881 tttcgtgccg gttccttgtt tcctgacatc cgtcatacaa ttccggtttc ctgctgacgc
     2941 ttcacaggga tggcaataat caccggggca tttgacgcgc atcaagaagt ccgcgcggta
     3001 ccggtaatat gtttgcagcg tatccggaaa acgggataca cagtaaaccg ataattaaaa
     3061 acaaacaact atgagaaacc ttacattaac aatgtttgct ttcctcctcg caatggcggg
     3121 attgaaggcg caggtaacgt gtacggatct gaacaattat gtggattcga aaaacacggg
     3181 ccctacgggt tattacaccc tgcaaaatgg ccaggaagaa atggccgcac aaacgtatca
     3241 ctattcggtt gccggaaaag tgaacagcgt acgcgtttac ggcaactatc ccggtgtagt
     3301 cggcggagtt ccgctgcgcg tgagcattgc caacgtagat gcgaacggaa gaccgacgac
     3361 cactattcaa tcagtaaatg taacctggtg gtggtttgac aacttctcca gctacatgac
     3421 ggtgaatttc cccggtggcg gagtaacggt aaataacaac tatgccatta ccgtcgagct
     3481 gcggaatgca tctccctggg gatcgtcttt ccagctccag tataccggcg acggtgaagg
     3541 actgggacag gatctcgcgt cacttgccgg aacatcgacg ggccataact ggaccagcgc
     3601 gatgaccaac ttcagcaagg acggagattt ctacctcgtt ccccgcatga cgcattacat
     3661 tacgcctgcg ttctcagtaa gcagcaactg cgcttctgtc ggaacgcccg taacgttctc
     3721 caaccagtcc atcatgacga cggacagcat gttcaacatc atcgggctga gcaactacag
     3781 cggcagcaat tacttttata cctggaactt cggtgatgcg tcaccggttt cacatgctgc
     3841 gaacccgaca cacacgtatg caactgcagg ttcttacacg gtcacgctta cctgcatgat
     3901 cgacggttgg tttacagacg gatcagctac gaaaacggat gtgatctctg taggtctcgg
     3961 ctcaaacgct acggctactt cggtcagctg caacggcgga tcgaacggaa cgattacgat
     4021 caatgcaacg ggaggttctt ctccttattc ctattcaatc aacgcaacag gtttccagcc
     4081 aggaaatatg ttcagcggtc tcggcgcagg aacgtataca acttacgtac aggataacgt
     4141 cggctgcatt tcaaccggct ctgttacgat cacacagcct tctgcaattg tgatcagcaa
     4201 cgtttcttca acgaattcca gctgcggcaa cgctgacgga gcgcttctca tttccgcaag
     4261 cggcggaacc ggaacacttc agtaccagct caacagcaac ccgtaccaat ccagcaattc
     4321 gtttacgggt ctcggctccg atttttacac agtaacagtg aaagacgcga acggctgcac
     4381 gaccagcacc aacgctgccg tgaacgacca gggcgctcct tcgcttacgc tcgtttccat
     4441 gacgaacatt tcctgcaact caggaagcaa cggaacgatc gttctgaacg gaacaggcgg
     4501 atccggtaca cttcagtaca gcaccaatgg cggtaacacc tggcagacaa acggttcatt
     4561 cctgaacctt cctgccggag attatcttgc tatggtaaaa gacgcaagcg gctgctcttc
     4621 cgcaatggag atcacgctgc acgaaccgcc ggcaatttct ttccaggtca gctctacacc
     4681 tgtaggctgc catggagata acgacggaac ggtcacagcg actgctgcaa caggcggaac
     4741 aggaacgtat tcttacagcc tcaataacgt caattaccag tcaggaagca tgttcacggg
     4801 agtcacagca ggaacctaca cggtttatgt ccgtgacatc gcaggatgtg ttgccacttc
     4861 ttccgtcacg gttaccgaac ccaccgcgct tacggccagc acctctgtta tcaatgcagg
     4921 atgcaacgga agctacgacg gcggaattac agctactgca acgggcggaa ctgctccgta
     4981 tatctacagc ctcaatggtg aagaatggca gtcttccggc acgttcgacg agctcgaaag
     5041 cggaacgtac atgatcacca ttcgtgatgc gaacggttgc caggtgctcg ttaacgcaac
     5101 gatcacccag cctacacttg ttactgcttc tatcacaacc ggtgcttcta cctgcggtaa
     5161 tgcaaacggt acacttctcg cagtcggcag cggcggaaca ggttcaggtt atacttacag
     5221 cattgacgga accaatttca acagcagcgg ttcattcagc agcctcacct ccggaacata
     5281 caacgtgatc gtacgcgacg gaaacggttg ccagaatgtg cttaccactt tcatcaatga
     5341 tgcaaacggt ccttccatca cttcagtcag cagcacgaac gtagcctgcc acgacggaac
     5401 agacggaacg atcactgtga attcagtaag cggcggaaca ggcacgctcc agtacagcgt
     5461 gggcacaacc tggcagcttt cgaattcctt cacaggactt gacgcaggaa actataacgt
     5521 tcttgtgcag gatgcaaacg gttgcgtagg ccagacttca gtcactctta cccagccgaa
     5581 cgctatcgtg gttacgactt catcaactga tgttacctgc cacggaagca ataacggaag
     5641 cgttattgtg aacgcagccg gcggcgcagg cacgctcgct tatgcgatcg acaacgaagc
     5701 tccgttccag tccagcaacg tgttcaacaa cctgtatgca ggtacgtatt acacgatcgt
     5761 gcgcgatgca gcgggctgcc ttggttcagc ttatttcacg gtgaacgaac cggccgcaat
     5821 tacgatgaac gtaagccagc tcaacgtagc gtgctacggt ggaaatgatg cagctatcta
     5881 catcatggca atgggcggaa caggtacgct tacctacagc cttaacggta tcaattacca
     5941 gtctgcaagc tcattcctca accttaccgc aggcaactac actgtttacg tgaaagatgc
     6001 caacggatgt gttgaaacgc agatggtgat catcacccag ccttccccgc ttgttatgaa
     6061 ttacacggtg agcgatgttg tatgctcagg cggtaatgac ggcgtgattg acctcagcat
     6121 cagcggtggt gttcacccgt actatttcga ctggtcgaac aaccagacga cggaagatat
     6181 cttcaatctt tctgccggta cgtattccgt aacgctgact gatggtaacg gttgtgtcta
     6241 ttcacaggcc ttcaccgtat cgcagcctgc aaatgcgctt attgtcaacg gtgttatcac
     6301 cgacgcaact tcacagtcaa ccaatgacgg cggagtaagc ctgacagtaa ccggcggaac
     6361 aggtccgtat acttacctgt ggagcaacgg cgctaccacg caggatattt ccaacgttgc
     6421 acccggcgtt tactctgtga tcgttacaga tgcaaacggc tgcaccagct caggaatctt
     6481 cctcgtgtct gtggtaagcg gtgttgtcaa ccactcgatt gacggcggcg tgaaacttta
     6541 cccgaacccg gcaaatgata acttcacgat cgacgcagga aattccgtga tcgaccgcgt
     6601 ggaaatctat gacatgctcg gccaagtagt gtttgcaaat gagccgaaga ctccgaaaac
     6661 acagatctct accgacggat tgagccaggg ctcctacttt gtacagatgt acatcggcgg
     6721 acaaaaggta acgatgcgtc tccaggtctc taaataaccg gagtttatcg ccccaggaag
     6781 agcaggcaga aatgcctgct ctttttttta ttccctgcag agccgcacct tcttgtttta
     6841 ctgtgcagag atcaatcgtt cgcatgacgt tcttcctttt atcatcacgg tccctgctgt
     6901 tcctttgttc taaaatcctg catgaaaacc gattggaaaa gtattctcat tgccgtattg
     6961 atcttcatca tcattgtttt ggtgtggttg ctcttcaaag cggatgccgg gcgttattgt
     7021 gtaccggcca accgttttca tccctgaaga gcagttccgg gagtcctaaa cgggtacaga
     7081 ttgataaaag tcatcctttc ctttgatgaa gtcgggtgcc ggtcattttg catcccacgg
     7141 tttggccata acgttgcgga acaaatttta ccaggaaaca caccaacatc cacacaccca
     7201 tgagcaatac aattctcctt attgaagaca accccgaaat gcgagaaaac acgaaagagc
     7261 ttctcgaact tgccggttat tcggtgaccg aagcgatcaa cggcaaggat ggtctcgaaa
     7321 aagcgcataa ccagaaacct gatcttatcc tgtgcgacgt gatgatgccc gagctggacg
     7381 gctatggcgt tctccgcgcg atgaacaacg ttccgcatct ttccggtgtt ccttttgttt
     7441 tcctgacggc gaaatccgag aaatccgatt tcaggctggg aatggacctc ggtgcggacg
     7501 attacctgac caaacctttc gagggagacg agctcctgcg cgtggtggaa gcccgcatac
     7561 ggcggagcaa cctgctgaag aaaaaatatg cgcagaacct cgaagggctg gatgaattta
     7621 tggcggaagc gcagtccatc ggggaactgg taaatctttc cagcgggaaa acggtgaaga
     7681 agctgcgcgc gaaagacatg gtttacatgg aaggcgatac ggcaaactat cttttcttcg
     7741 tggtgagcgg ccggatcaag accagcaaaa caaacgaatg gggaaaagag ctgctcctcg
     7801 atatttattc agccggtgat ttcttcggtt accaggcgat gcttgaagaa ggcgcttacc
     7861 agcaatcggc gcaggcgatc gagaacagcg aagtagcgct catcccgaaa aaagattttc
     7921 tcgctctgct gtattcaagc cgcgaagttt cactgcagtt tatccggatg atgtcgaaca
     7981 atatgaatac ggcggaaaca aagctcctca agctggcgta cgactccgcc cggaaacgcg
     8041 tggcagaagc gctcctgctc atcgcgaaaa aatacgagca gaaatcttcg ggagaagttt
     8101 cttttcccgg gctccgtgaa aatatttcag gtatcgccgg tatttcaccc gaatcggtga
     8161 gccgcaacct gaccgatttc cgcgaggaag gactgatcga aaccgataac ggggagatcc
     8221 gcataccgga cattaaaaaa ctggaaaagc tcaggttctg accagcactt tcgcggcgga
     8281 acaataggcc gcggatggtt tccgtgtttg cggtgtgtga tgaccggcgt catgcgctcc
     8341 tgtgatctct ttcctcgcaa aacttttgtg cacgaactta ttttgtaacc gaaaaaaatc
     8401 cctcatgcaa atgcataccg ccgtttcacc gcaatattgc cgcgtacgcc cgatggaagc
     8461 cggaaagaaa aaacccgaaa tagagcctaa accggtcgaa ccggttactc ccccggcccc
     8521 ggaaattact ccgctcccgg aaaaaacaga acctgtccgc aacccgcctg aaattacgcc
     8581 ggagccggag cctgaagttt ccccgcagcg ggaaaatgaa taagtgtttt ttttcctttc
     8641 tccggtttcg tgtcgtctgt gagttccccc atcaccgcga catgttcata ttccccacgg
     8701 gtatgtttcc gggacctggc tttcactcgc aggaacatga agcgggtgca gcgctttcgt
     8761 ctggtcaata aaaacaaacg cttcataacg taaaggaata atgctcggca cataattacc
     8821 gtgctgttca tattccgggt gataaacgac cccgatagcg cgatgcccca gcgcttcacg
     8881 gaaaaaatgt ttctttttca ggctttcggt aagcaggtaa aaaacagtca tacctgtccg
     8941 gtgcagcagc tcttcccagc ttccctccat ggcgcgcggg acattcatgc gttccatttt
     9001 ttcgccccac tctttccctg cgataacact cccgctgtat gaaccgaaac ctacacggaa
     9061 cactttccgg ctgccgtatt tctcattgag cagctgaccc acattcacca tgcctccggc
     9121 cgacatggcg gttgcgcgcg catcgccgat gtgcgtattg tgctcccaca cgatcgcttt
     9181 cgatcctgtc ccgtgaaaat tgacaagacg ttcaagcgtt tcggtcatgt gatgatcacg
     9241 cacattccat gaagaaatcc cgcccttcat cattgtccgg taataatgtt ctgcgtgaac
     9301 tgcaaccacc gcattctgct cggcgttcaa agcgccttcc ggatcggtat tgtaatgctt
     9361 cattcccctc ctgacggtcg caagcaggtc cgtcacttct tcctcgcagg aggcaggaat
     9421 gagcgcagcc gcctgcgcgt aggagcgacc ttcgtcatgc ccgaacgaat caaagcaccg
     9481 caccgctttc cgcgctgctt ccgcagcaac acgatccgtc cgttccaggt attttatgat
     9541 cgcttctatg gattcccaaa ggctatacac atcgagtccg taaaatcctg cttttcttcc
     9601 cggcgcctgg ttccggttga acttatgaag ccattccgca agcgcgacag tttcccagtt
     9661 cgcccacatc cacgtgggcc agcggttgaa ttcctgcagg acttcctttg cggaattccc
     9721 ggcatccgcg tatcctttca catacctgtt caggcggtaa caatccggcc agtcgccttc
     9781 taccgcaacg aaagaaaaat tcttttccgc gataaggcgc ctggtaatgt gcgtgcgcca
     9841 ggtataatat tcgtgcgtac cgtgcgaagc ctcgccgaga agcacatatt gcgcgtcccc
     9901 tatcgcctcc atcaacgcat cgagaccgga tgcattttcc agtccgtggg cgcattcaca
     9961 gatgaaatcc gcagcttcct gctttcccgg catttcttcg ttccatttat ttcctcctct
    10021 tgttctcatg attcctgttt tttcacaaat aacaactatc cgcactgcgg ggccaattca
    10081 tcaaatcatg atgcgccgtg atgtatatca aagcgaatgg aacgcaaccc tgatagctta
    10141 gcgctcgtat gtcaataaaa ctgtacagag aaatgcgaac cctccttttc agcaaccgtg
    10201 cagaagccgg cttgcgatta gctggagcgc tggagaaatt ccggggcacc gacagcgtta
    10261 ttctcgccat cccgcgcgga ggtgtggaag tagctaaagt ggtagctgaa acactttccc
    10321 ttccgctcga cattgcgctg tccaaaaaga tcgggcatcc tgccaacagc gaatacgcga
    10381 tcggttccgt atcgcccgac acggtcgtgc tcgatgaaca cgcggacgtt tcgcaggaat
    10441 acatcctgcg cgaaacgaaa aagatccgcg agctcctgca gaaaagatat gatctctaca
    10501 tgggcgcccg aaaacctgta gcgctgaagg gtcgcacggc gattgtagtg gacgacggga
    10561 tagccaccgg aaatacgttg cttgcaacga tacgcacgct gcgcaaaaaa gaaccgcgga
    10621 gcatagtcgt cgcagtgccg gtcgctccgt cacgtggaac agaacggata aaaacggaag
    10681 ccgatgaatt tatttgtctt tacactcccg ctgatttccc gggcgtaagt gcattttatg
    10741 cagattttgc ccaggtgagc gatgaagaag tcgtgcagct gctgagcgaa aacgctccac
    10801 acgggtcgga aaaagaaatt acatgaacgc tgaagagatc ttcattccca tcggggacat
    10861 aacgcttccc gctttgctcg acgtcccggg aaaggcagga ggtgtcgtga tcttttcaca
    10921 cggaagcggg agcagccggc tgagcacaag aaaccggttt gtggcggaaa tccttcaccg
    10981 taaaaatttc gcaacgctgc tgttcgacct cctgacggaa gaagaggatc gcatttatga
    11041 aaaccgtttt gacatcgatc ttctcacgca gcgccttatt tccgttacac gctggatcaa
    11101 aaaaattccg ggcacaaagg acctgcgtac cggttatttc ggcgccagca cgggttccgc
    11161 ttcggcgctg aaagctgctt cgcacctggg gaatgaaatt ggcgccgtcg tttcacgagg
    11221 cggacgtccc gatcttgccg gcgatatccg cggcgtcacc gctcccaccc tttttattgt
    11281 cgggggactt gatacttcag tcattcttct aaaccgcgaa gcgtccgcgc atcttacctg
    11341 cgtgcacgag atgaggatca tcgagggcgc ttcacatctt ttcgaagaaa agggaaagct
    11401 ttccgaagtt gcaaaagaag ctgcggaatg gtatgaaaaa taccttacgg cataaattat
    11461 tcttccacct tttttatcat gcatgcatca cgagcaccgg gtaagctgcc gtaagctctt
    11521 cttcgctgcc gtgtttttcg cgcaatattt cccgcagcat ttttcctttc tgaaccgcca
    11581 gcgcgagaac agcgctttcc tttccgcgca tgtattcatc aatgctgtcc tgcggatgcg
    11641 gagcgataat acgttcgaac ttcgcttcgc cttcactgaa cacttctccc actttttctt
    11701 tcagcgcatt gaaaatatcc ttgcttacgt cactgatgtg cctgctcacg tgcaggaagg
    11761 tgatctgcgc accgagcatt ccggccagct ctttcagccg gacaaataaa ggcctgcttt
    11821 ccggatcata gtcccacgca aaaacgattt ccgtgagtgt acggtattcc acctgttccg
    11881 gtacaaccag cacgggacat cccgcatgca cggcaagatt ataagcgttg gatccgaaaa
    11941 aatcctggaa gagatcgtct gcgccgttag tgccggcaac aataagatca cccggaccgg
    12001 cttttccagc tatcgcttcc gtgagggttt cctcaccggt ttccacgccc gttccgcagg
    12061 agatgctgaa cattttattc aattcggcag cgagattttc cagctcgcgc gccactttca
    12121 tcacatgcgc ctctgcatcc gctttttttc ttcccgcaac cgccatgagg gcccgcgaag
    12181 aatgatactg gaaatagacg agctccagcc cggccccgct caactgtgcg agctttgccg
    12241 catattgtac ggcgttgcgt gctgccggag aaaaatcaat gggacaaacg atccttcgta
    12301 acatgatttt gttttttatt catcgctgac gccggagccg gtgatttcct ttttccgtat
    12361 tgcgccgttg atgaggatga actcgttaat attctgcaga tcgatcacac cggccagcct
    12421 gccgttttcc atcacgggac acaggtactg acctgctgca agcatcttct gcatggcatc
    12481 ttccaggtgc atttgcggcg caagcgaaag aaaatctctc cgcatcacgt ccgcaacccg
    12541 ggtatgtttc ccgtcctggc tcagtccgtg cacaagctct ttctgcgtga gaataccggt
    12601 aacattcccg ttctccgtta cgacaaagct ttgctcgctg ccatcgagca ttttcgcgct
    12661 gcgctttcca ggtcttcttc cggcgaaaga caggtaaacc tgtgcatcaa tacattccgc
    12721 acatggtaat ggctcagcaa tgattttgtc atttctgccg tcgcctcgct tgttgcgccg
    12781 aggaatacga acacaccgat gaatgtcagc cagacattca caaaaagccc ggtgataaca
    12841 aatacaacag cgatcacctg cgccacattc gccgcgatct tcgtcgcgcg ttcgcgggga
    12901 aaccgcatgg caagaatggc acgcagcaca cgaccgccat ccatcgggaa ggcgggaata
    12961 agattgaaga aagccacgac aagattggcc gtcatcagca cgtaaagaaa attatcgcgc
    13021 gtcagcgtag tgatcaccgc ggacgatgga agtcctttcg atatccaaag gaaaagccag
    13081 atgaacgcgg cgatgacaaa gttcacccag ggtcctgcaa gcgccatcag catttcctgc
    13141 aacggcttct ccgggatctt gtccatcgac gcgacaccgc cgatggggag cagagtgatc
    13201 tttttcgaat agcagccgta cctccgggca accgtgatat gcccgaactc gtgcagtgta
    13261 atgcaggcga aaaagacaag tacgaataaa acagaaatga tcccctggcc gagatccccg
    13321 tgttccctgt acgctgaaat aaacacccac gcgatcagca ggaaaaacgt ccagtgaacg
    13381 aatatcttcg tttctcttat ggcgccgata tacaatgatc ctctcagaaa attcatgttc
    13441 atttttttta gcggggcggt gatccagaaa acaaaaagag gcttacgggc ctccttttat
    13501 ttcatcaccg ggcttatgcc acttcgactt cttttttcgc tttcggcgac ccgggaactt
    13561 ttttcgccag tgtcagcttc agcacgccgt cgtcgtattt tgccgcgacg ttttcttcat
    13621 tggaattttc cgggagggaa aatgaacggc tgaacgaatt atagctgaat tcgcggcgcg
    13681 tgtaattttt ttccttctct tccttttccg ttttcttttc tgcggaaata ttcaggatcc
    13741 cgttttcgac attcaccttg aagtccgatt ttttgaaacc gggagccgca agctcaatgc
    13801 tgaaatcctt ttcattttcc acgacattaa cagcgggaac ccattcccca tccgttaccg
    13861 aaagaaaagg ttcgcggaaa aagcggtccg catcgaaaaa gtctgaaaaa aaagtacggg
    13921 ttgaaggggc gttgccgttg ttgcgtttaa tgagtgacat ggtagttgtt ttttagttga
    13981 acaataggtt tgttttgctc accaaaatta ttctaccccc gccggcattt ccctgatgcc
    14041 gatcaaatga atacgtgatt ccccgcacat tcacgcatac gactgtaaac tatgcacgca
    14101 gcaccgggtt gtcgttacac caattgtatt tacaggaatg ccggagacgg atcatttata
    14161 caccgacacg tggctgatct tccgcacggt gttttccccg gagcattgcg tggtgaaatc
    14221 gatcacgacg acataaacat cattggggac tttgtcgcct ttgtacgttc cgtcccatcc
    14281 ctgtcccatg tcatagcttt cgaagatcat ctcgccccag cgatcgaaga tcatcatgtg
    14341 aaaagaaata atatcgacac cttccgcgta aaaaatatca ttcttcccgt cgtggttcgg
    14401 tgtaaaacaa ttcggaacgt aaaccgtgga aggaccgaac ccgccgatca cgtcgatcgt
    14461 atccgtgagc atgcaattgc cggtgctgat gttcacccaa tatgtgcccg cttcattcac
    14521 ctcgatcgaa aatgtagtgt cacctgtcga ccacaggtac gacgcaccgc ccaggttttc
    14581 agagacaaga tggatcccgg cgttgtcgca gagattgaca tccggcccga gatcgaacgg
    14641 agcgattgcc tggacgttga cagaatccga tgcgctgcac ccgctgttcc acaccgtgac
    14701 gtaatacgtt ccgccggcgg aaaccgtaat gctgtcttca atggaattgt tgctccacaa
    14761 ataggtgtcg ccgggattac cggcattgag cgtcgctgtt tgtcccgggc agagcgaaag
    14821 atcaggacca agatcaacct gcggcacagc gacaaaattg acgatgatcg aatccgaaag
    14881 ctggcacgtt cccgacgtca ctgtcacgga atacgttccc gaactgttca cactgatcgt
    14941 ctgcgtggct gcacccgtgc tccatgcgta ggtgcttccc gggtttcctg catcgagcgt
    15001 aaccgggttt ccttcgcata acgtcgtatc tcctccgaga tcaggcggag tgaatacgct
    15061 taccgtgatc gtatccttat cgctgcacac gccgttgccg gccgtcaccc agtaagtacc
    15121 tgcgctgttc acattgatcg tctgcgaagt cgcgcccgtg ttccatgtgt attgcgcgcc
    15181 cgggttaccc gcgtcaagcg tgaggttcgg cgttccgcaa aacgtcgtgt caggcccgag
    15241 atcaacaacc ggcacgggca aaacagtaac atagatctgc gtcgtgtcgg atgtgttgca
    15301 cccgagcgaa tcgatcgcga taagcgtaac cgtgtaagag ccgggcgttg tgtactggtg
    15361 cgaaggcgcc gtactcgtat cggccgggct tccgtccccg aaattccatt cgtaatcctg
    15421 tgcgccgatg gaattgttcg caaagctcat cgtcgtaccg aggcagatgc tgtccgacgg
    15481 agaagcaacg gcaaccgcgc tgaggttcac cagctggaaa tccattttga tgaccgcgtt
    15541 gttgcagttg aagctgttat tcgtcgtcga ccatgcgccg ggcgttgtcg gaaagttctg
    15601 cgagccgccg caacctgcgc agaccgactg gtagatcacg ccgcgtttat cgaaacggct
    15661 tgtgccgccg tccacatgct catccgaacc gaggtttccg ccgaagtaag ttgcgtacca
    15721 aagcgagatc gcgtttttct tcagcacgaa aaaataaaaa tcgcatccgt ccgtcgtcga
    15781 ctggaacgcg ttcgaagtga cgggcagacc aaccgttgtg cccgtgcttc cgtagggaat
    15841 gcattgtccg ccccaacccg ccgcgtacac attctcgcag gtgtcgacaa gaaaagcgct
    15901 gggcgaaata ttcggcgtgc ctgttcctga tccaaaaaca gtggagtaaa acgtggagct
    15961 cagcgatgca ttcatcttgt gaatgaactg cccgctgttc gggttggaat acacacctgc
    16021 gctcaccggg taggcgcccg ccgtctggcc gtacacatac acgttgctgc tgttgtcgag
    16081 ctgaatgaaa tacgcctggt cgtaaccgtt cgtgccgatg aacgtcgact gcaacatggc
    16141 tacgcccgaa ggatcgagat gggaaataaa tccgtctgcc gtgccgccct ggaaagtcgt
    16201 gtggagcgtt cccggtgtcg tcgggaaatt tgaactgctg gttccgccgg aaacataaac
    16261 gctgttcgtt ccatcgagct ggatggagta aaccgcatca tcgccgctgc cgccgaggaa
    16321 cgtgctccat gtcatcgacg tgaggttgct gttgagcttg aacacgcagc cgtcctgcgt
    16381 tccgccgccg aatgaagact ggtaagcgcc gccggtgacc gggaaattcg ccgactgcgt
    16441 ggaagatgcg atgtagcagt tgcccgcatt gtcggtgatg atatcgctgc ggccgtcatc
    16501 gccgtaatta tatttcagcg acatccacgt gtaaaaattc gcggtgatgt tcacgccgtc
    16561 gtcaccgctt ccgccgacga acgtcgaacc gacaagtgca gttcccgtgg aattgaattt
    16621 cgtcacgatg atatccgcgt tgccgttgta gctcacgtcg tacgcgccgg cagtaacggg
    16681 aaaattcgaa gaatacgttc tgccgatcac gcacagctcg tcgttgctgt ccacgacaat
    16741 actctgcggc tgttcgttat cggatccgcc gaggtaagtg gaaaagagaa gcgcggtacc
    16801 ggacggattg tattttgtga gcgtgatgtc gaacgggtaa gcatttccgc cggagccacc
    16861 gcctccgaat gtcgtctgga atgcgccggc ggttgtcggg taaccgatcg ccgtgccgat
    16921 gccgccggaa taaatatcgc cctgcgcgtc atacgttgcc gtgtaacccc agttatcgga
    16981 cgtggagccg ctgaacgtag aacagaccag cgtgggatcg atcacaagcg gccaggcatg
    17041 atcatatcca tccgggaact cgaaggaagc gaccgttccg tgcaggacat aacggcacct
    17101 cactgctgtt tcccttccgt cgataacctg gtatgcatac gggcgctgtt ccgtcacatc
    17161 gccgagcgaa gtgtgaatga cgaggttccc gtcgttcaat gaaactttgt ccgcacccgc
    17221 ataattgatg cggatcacgg acggatcgcc atcctcatcg actttaaaat catattcaag
    17281 atttccgtca cggctgtaga gatgaacgcc caccgaagga tagagctcat tgtaatccat
    17341 ctcatcatac aggtgaactt cgggcgccca tttcgccggg tcatttccca aaaaataatt
    17401 cttgtaatac ggagcaggaa gaccggccga aatgaacgga tcactgttcg ccccgagaaa
    17461 ttccgcacgg aagcaatggt aatgaaccgt gacatcctgg tccggggaaa taacgccgtt
    17521 caccggtgac gggtgatgaa gctgttcgat ctcatcggcg ttccagaaaa gatacgtgaa
    17581 cgcattcttt tcgaaaaaaa cctttccgct gcgtgtaaga tcagcttcgt aaacaacacg
    17641 cggatcgaac tggtttttat tctcctcgaa cctgagcagg ggcgcatcca gcttctgaac
    17701 gggagcagaa ttattcccgg caaaaagcgc aggactgatt gcaaacgtga agatgataaa
    17761 cggaaatatc cgtgccgtgg acatttgagg gacagcttaa cgctgtggaa ttgagaggaa
    17821 aaggtataaa aaaaacagat acctctttat cccaaaatcc gcaggtctat tttacagtaa
    17881 acacccgcgg atttttcacc agggcagagg ccttgcgttc agcaaattag gtcggtagaa
    17941 tttcggcgaa atgagtcctc atatcggtga aataacccct cataacggat tgcgtatata
    18001 acttgtggcg gttttcgaag cactttcttg tccgcttagc acaatgtttc ttagaggctc
    18061 aaacctttga tttaccactt caactgctat tgccacatac acgctgttac ctgctgcccg
    18121 actgctccct cgatgaagtg gcggtgcaag tttgcagtca tctcgtttta tttggttgct
    18181 aataatcatt tctgtcttgc aagttttagt gtccaaggaa tgtaaatcat tttgatgtcc
    18241 tgctctttta cgttcatact taaacgcaac atgtttttgg gaacacatag ttcgttgcga
    18301 attcgctcta aaaattgctc aaagtttact gatatggtat taaactcatt gtcatcatat
    18361 ggttcaagta attcaacttc tttgtgctta atagaaatta gtttgtgtgc aattcgtttt
    18421 acc
//
LOCUS       DASTSF010000406         2140 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1423145,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000406 DASTSF010000000
VERSION     DASTSF010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2140
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1423145"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..855
                     /locus_tag="VFU15_11665"
     CDS             <1..855
                     /locus_tag="VFU15_11665"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEU4718488.1"
                     /translation="TLDPEPNPLLKTINWPLPTLQYPVLNSIGVDWVHGAYANQAAYH
                     GWYGFRITNPSSPILAGSGLSYHSTLSCKSNEDDGALIAGFNSVSDPILDSVSLGFCR
                     IELIGYDYGESVYYPNSPQTGYCTFIALHKAPAAGNIINVASSNWCGENTGSSSGGFG
                     GPDSVKVQIITNNMLNLLLTGSNIFSDSSGCVLSSDSYADNARSSGNVFPNPSSGQLT
                     IRRNVPEDKTGTVDFYDQSGRLVLTRVLSGEKQQTVDISQLAEGTYFYTVRTEGENPE
                     TGKLVLIH"
     gene            complement(1022..1408)
                     /locus_tag="VFU15_11670"
     CDS             complement(1022..1408)
                     /locus_tag="VFU15_11670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU4718489.1"
                     /translation="MDKNVNSLNWFEIPATDITRAKKFYESIFKMEMTDMGEMMGMKM
                     AGFPAEMGNGKVSGALVQGPMHKPSADGSVIYLNAGSNVQGVLERIEQSGGKVVMPRT
                     QISPEIGYMAFFMDSEGNRMALHGQN"
     gene            complement(1528..>2140)
                     /locus_tag="VFU15_11675"
     CDS             complement(1528..>2140)
                     /locus_tag="VFU15_11675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718490.1"
                     /translation="IYERLNFPMGGDSVRYNAKGYVAQGTWEDYYKSGAVLHTGYYID
                     GQLRSYKNFYPDGKEEREFKQLDYFRYQMTLWYPDGKVRSDITYFNGQEEVTHEYYAN
                     GNPEFAEEYAKKLAYLMYRTFYYENGKIQSDMQLLDKKKQKYSYKEYYENGNLQDEGV
                     MQYYPEVDNYLKEGTWKNYDENGKLVSIQEYVRGQMTDEKKVD"
BASE COUNT          484 a          526 c          539 g          591 t
ORIGIN      
        1 acgctcgatc ccgaaccgaa cccgctgctg aaaacgatca actggccgct gccgactctt
       61 caataccctg tgcttaacag catcggtgtg gactgggtac atggcgccta tgcgaaccag
      121 gcagcttatc atggctggta cggattcaga attaccaacc cttcttctcc tatacttgcg
      181 ggcagcggac tgtcatacca cagcacgctc agctgcaagt cgaatgaaga tgatggcgcg
      241 ctgattgcgg gatttaattc tgtgagcgat cctattctag attccgtttc tctcggtttc
      301 tgcagaattg agctgatcgg ttatgattac ggggaaagcg tttattaccc caactccccg
      361 cagacaggtt attgtacatt catcgccctg cacaaagcgc cggctgctgg taatattata
      421 aatgttgcat cttcgaattg gtgtggagaa aatacgggat cttcttcagg aggattcggt
      481 ggaccagaca gtgtgaaggt gcagatcatt acgaacaaca tgctgaatct gctgcttacg
      541 gggtcaaata ttttttccga ttcatccgga tgtgtgcttt cgtctgattc gtatgcagac
      601 aatgcgcggt cctccggaaa cgtatttcca aatccttctt ccggtcaatt gacgatccgc
      661 cggaatgttc ctgaagacaa aaccggtacg gttgattttt atgaccagtc cgggcgcctt
      721 gtgctgacgc gtgttttatc aggtgaaaag caacagaccg tagatatttc acagctggcg
      781 gaggggactt atttctatac agtccggaca gaaggggaga acccggaaac gggaaagctt
      841 gttcttattc attaaaacct caaagcgcga agaaaagtgt ccgcctgatt tgatgaatgc
      901 attgcgtttt taacgcaggt atttagggac ggaccggaag aataggatgc ttatcaaata
      961 ggattttttc gaaaaaaaac cgggatcgcc ccggtttttt cattggtgaa aaggagatcg
     1021 ctcagttctg cccgtgcagc gccatgcggt tgccttcgct gtccatgaaa aaagccatgt
     1081 aaccgatctc gggcgagatc tgtgtacgcg gcattaccac ttttccaccc gactgttcga
     1141 tgcgttcgag gacgccctgt acgttcgagc cggcgttgag gtagatcacg gaaccgtccg
     1201 cagagggttt gtgcatggga ccctgcacga gcgcgccgga aacttttccg ttgcccattt
     1261 cagcagggaa gcccgccatc ttcatgccca tcatttcgcc catgtcggtc atttccattt
     1321 tgaaaatgga ttcatagaat ttttttgcgc gcgtgatgtc ggttgcagga atttcgaacc
     1381 agttcaggga atttacgttt ttgtccatgg tgttttcgtt ttatagtgga ccgtaaatgt
     1441 agcgaccggc gccatagcgc gtattaaaaa atgatgaatg aaaaatgatg aatgacgagt
     1501 ggcagcaggc cgggcgggaa cgggaactca atcgactttc ttctcgtcgg tcatctgccc
     1561 gcggacgtat tcctggatgg agaccagctt tccgttctcg tcgtaatttt tccaggtgcc
     1621 ttctttcagg taattgtcga cctcagggta atactgcatc acgccttcgt cctgcaggtt
     1681 cccgttctca taatattctt tgtacgaata cttctgcttt ttcttgtcca gcagctgcat
     1741 gtcgctctgg atcttgccgt tctcataata gaacgtgcgg tacatcaggt acgcgagctt
     1801 cttggcgtat tcttccgcaa attcaggatt tccgttggcg taatattcgt gcgtcacttc
     1861 ttcctggccg ttgaagtagg tgatgtcgga gcgcactttc ccgtcggggt accacaacgt
     1921 catctggtaa cggaaataat cgagctgctt gaattcgcgt tcttccttcc cgtcgggata
     1981 aaaattcttg tacgaccgca gctgcccgtc gatgtaatag cccgtatgca gcacggctcc
     2041 cgatttgtaa taatcttccc acgttccctg ggccacgtag ccttttgcat tgtaacggac
     2101 cgaatcgccg cccatgggga aattcagtcg ctcgtatatg
//
LOCUS       DASTSF010000407        12962 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1591071,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000407 DASTSF010000000
VERSION     DASTSF010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 12962)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12962)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12962
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1591071"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            39..2015
                     /locus_tag="VFU15_11680"
     CDS             39..2015
                     /locus_tag="VFU15_11680"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEU4718491.1"
                     /translation="MKRFLLHLAFLVTAVAGCKAQPPTQLSTRSKKAEKAYNKALAEL
                     NAYQYDLALADLAVAKKADPNFVEAYFLEANLYMEQQEWQKAIDDFKTGFSINPKYYP
                     SCYFDCANAELKLGQYEGAKTDYQKFLDLKRNASQDMIDKTNLGIKCCDFALDAMQHP
                     VPFKPVNMGPNINSDWCEYFPNVTADDQTFLVTRNQQKIDPNNGDKIYTQEDFYISYK
                     NDSGQWSFMRNMGAPINTPENEGAPSLSPDGRFLFFAGCMGENGYGYDRRGYGSCDIF
                     YTQKMNGSWTRPVNVGAPVNTKEWESQPSFSSDGKTLYFVSSRGGGYGDSDIWMSTLN
                     DDGTWSEPVNLGDVINTPGKEEAVFIHPDNQTLYFASDGHVGMGGLDLYVSHRDSVTG
                     KWGEPVNLGYPINTYGDESGLIVGGNGQIAYFSSDRKGGLGCDDIYSFELPKALQPFP
                     VTYMKGKTFNKKTGAPVGAKFDLIDLATGKTFITSKSDAANGEFLVCLPTNKDYALNV
                     NSPGFLFYSETFRLKESPDPDKPFIMNVPLQPIDTGAVVELKNVFFETGKFDLRPESK
                     AELDKAVAWLLANPDVKVEISGHTDNVGDKKSNMTLSTNRAKAVYDYLVAHGVPASRM
                     TYKGYGDTKPKVDNDTPEHRQMNRRVEMKITATK"
     gene            2202..2978
                     /locus_tag="VFU15_11685"
     CDS             2202..2978
                     /locus_tag="VFU15_11685"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU4718492.1"
                     /translation="MTKWSQINAWLHYRKTSVTKHGVHSPFVFTLATKVFPGPKNSDP
                     EEHPAEDYRTELIMSNRVIDVQDFGTGISGPRKVAAIAKHSAKSPKEGQLLHRIVKHF
                     GPKKMLELGTSLGITTIYEATATPFEKFITLEGCPNTASLAEEIFRKENLHVEVRTGE
                     FGTTLKNALDSLENVDYVFFDGNHREEATLDYFETCLPYANNDSVFVFDDIHWSPEME
                     NAWEKIKTDPRVRLSIDVFHFGLVFFREEQMEKEDFVLRY"
     gene            3239..3397
                     /locus_tag="VFU15_11690"
     CDS             3239..3397
                     /locus_tag="VFU15_11690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002979933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718493.1"
                     /translation="MKINAAWHEKNKMPRNPTIEERIRWHVAHAKHCGCRPIPEKLQE
                     EMKKRKRK"
     gene            3394..4131
                     /locus_tag="VFU15_11695"
     CDS             3394..4131
                     /locus_tag="VFU15_11695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017258671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU4718494.1"
                     /translation="MNGNNQEIISLSGISREYRVGTEIIYALRNISLKIYKNEYVALM
                     GPSGSGKSTLMNVLGCLDTPSHGEYILNGTSVEKMSDNQLAEVRNKEIGFVFQTFNLL
                     PRSSALENVMLPLIYAGLSKAERQQKGEYALEQVGLKDRMHHKPNELSGGQRQRVAIA
                     RALVNNPAIILADEPTGNLDSKTSVEIMGLFEAIHKSGNTIIVVTHEEDIAKYAHRIV
                     RIKDGLVENDQLNNDIRQVNAPAFSDH"
     gene            4183..4731
                     /locus_tag="VFU15_11700"
     CDS             4183..4731
                     /locus_tag="VFU15_11700"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013684916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HEU4718495.1"
                     /translation="MKIYTKTGDKGQTSLIGGTRVPKHHIRIEAYGTVDELNSWIGLL
                     RDMTQDAHVKEILLETQDRLFTVGSLLASDPEKSKMKLPEIQDSDITLLEKEIDAMDQ
                     LLPPMKSFILPGGHVTVSHCHVARCVCRRAERNITHLSETAPVDELIVRYLNRLSDYL
                     FMLSRKLSHDLGAEEIPWKARM"
     gene            4979..5200
                     /locus_tag="VFU15_11705"
     CDS             4979..5200
                     /locus_tag="VFU15_11705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002996718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2795 domain-containing protein"
                     /protein_id="HEU4718496.1"
                     /translation="MYWTLELASYLEDAPWPATKEELIDYAIRSGAPMEVIENLQEIE
                     DEGEAYESIEEIWPDYPTKEDFFFNEDEY"
     gene            5370..5717
                     /locus_tag="VFU15_11710"
     CDS             5370..5717
                     /locus_tag="VFU15_11710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718497.1"
                     /translation="MMKNRHRHALPLLATLAGCVFMLAWVDPAHATEVTRQLTQKGFV
                     ISQLRKSEVAIVRNSGAQLKVMFRGFDSPCAGPCTTVTELLPRNTHHYLFIFPRFVKL
                     SLQDGIRTWMKRE"
     gene            5835..9179
                     /gene="secA"
                     /locus_tag="VFU15_11715"
     CDS             5835..9179
                     /gene="secA"
                     /locus_tag="VFU15_11715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002998448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEU4718498.1"
                     /translation="MFDFISKGISRLVGGSKSERDVKQIMPIIGEINEHFESFKSLSH
                     DELRNKTKEFRSRIAEYISEEENEIRELRAKSESPDISIDEQEDIFNRIDEIEKTILK
                     KIEEVLAEIQAEAFAVVKETARRFMENTEIVVTATDHDRNLSVKKPNVVIDGDKAIWK
                     NTWIAAGNEVTWNMLHYDVQLIGGTVLHQGKISEMATGEGKTLVATLPVYLNALPGRG
                     VHLVTVNNYLARRDAEWNGPLFEFLGLTVDCIDLHEPNSQERRNAYLADITYGTNNEY
                     GFDYLRDNMARQPEELVQRKHHYAIVDEVDSVLIDDARTPLIIAGPTPRNNNHMFTEL
                     KPKVEKLVAAQKTFINTILADTKRLFGEAAKSKEKEKEAGLLLLRCFRGLPKNKALIK
                     FLSEPGVKALLQKTEGVYLQDNQREMHKVDEALYFVIDEKNNTIELTEKGIDLLSTSA
                     EDHSFFILPDLALDLSELEKTYPDEKERLTKKEELIRDYNVKSERVHSMQQLLKAYCL
                     FERDDEYIVADGKVKIVDEQTGRVLEGRRYSDGLHQAIEAKENVKIEAATQTYATITL
                     QNYFRMYHKLSGMTGTAETEAQEFWTIYKLDVVTIPTNRPITRKDLEDLVYKTKREKY
                     NAVIDEIVKCVEAGRPVLVGTTSVEISELLSRMLKLRNIKHNVLNAKLHQKEADIVAE
                     AGKAGTVTIATNMAGRGTDIKLGPGVKEAGGLAIIGTERHESRRVDRQLRGRAGRQGD
                     PGSSQFFVSLEDDLMRLFGSDRIARLMDRMGLEEGEVIQHSMITKSIERAQRKVEENH
                     FGTRKRLIEYDDVMNAQREVIYKRRRHALFGERLQIDISNMIHDVTENIVVNNHESEN
                     YPQFELDMLRVFGMEAPVKEEDFKAARPAELAPQVTDTVSAFYREKSANMAARSFPVI
                     KNVFETQSANYENIVTPVTDGIRTLQVIANLKRCYETQGRELVFTFEKAAILAMIDDA
                     WKEHLREMDDLKTSVQNAVYEQKDPLLIYKFESFDLFKRMVDETNRDVISFLFKGNLP
                     GNENEQRIQEARPRAPQPQPKLQTIHEEAGGGSQQQAQQQQGRPAMPQQAPQPVQQVI
                     RQDPKIGRNDPCPCGSGKKYKNCHGK"
     gene            9288..10634
                     /locus_tag="VFU15_11720"
     CDS             9288..10634
                     /locus_tag="VFU15_11720"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEU4718499.1"
                     /translation="MNRRHFLRQSALISAGALLLPPALLEACRKKSADEDIGFGGKVL
                     IIGAGAAGLYAGYILRSKGIDFEILEASSAYGGRVGKINGFADFPLDTGAQWLHGKNN
                     ILGDLIAKTQTEITLDDSAEFFWFNNQITGTLPDDIEAIFLREDNVPDVSFKDFAVQE
                     GFGNEYADIVEGIAGDSGAAASRISAYWKIQEEAAWNSGDDDFKFRGTYFDFIDTNIA
                     AQVKDRIQLNTIVSKIDYSQSSVVVTDSNNNTYTADKVIVTVPVTILKAGDIEFVPAL
                     TQEKTDAFAKIGMDAGMKVFLKFSSAFYHQNIIGGNTCGAYADEIIGKTGSDHVLLAF
                     IMGEQAERLTALGSDSAITSALLQELDTMYSGQATASFIGSQVINWTTHPFIRGAYSY
                     STVGMGNARETAAQPVDKKIFFAGEAMNYNGNHQTVFGAVETGYREVINILNSVKK"
     gene            10631..11308
                     /locus_tag="VFU15_11725"
     CDS             10631..11308
                     /locus_tag="VFU15_11725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718500.1"
                     /translation="MKKLIAAFVLLLFCRCSFALENKLELSYTYLYSGQWDKAIQAYN
                     FSRPFLANQQPLFMNGLNASASHIFKPHKVFSYGITVSYLYFRSSVKNENFLNTLNLH
                     FADIGWLVHFENTEKPDGFYSELAFSAVSSGLFRTVNRKSFRYDGARAKAFGIGGDIH
                     LKAGYEFSAKNNFFISPFAALGYTPFLFSPNTEGVINQTTGLTGRRWTGIVQVQAGIA
                     LHWKKET"
     gene            complement(11556..12788)
                     /locus_tag="VFU15_11730"
     CDS             complement(11556..12788)
                     /locus_tag="VFU15_11730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002703134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEU4718501.1"
                     /translation="MSTYSRKDFLKLAGLASASFLLPKFLRGNDAQSEEMMKQLFLSG
                     TNGKRIVVIQLSGGNDGLNTIIPFGDDLYHSNRPSIGLNENQVIRIDDHFSFNGALQG
                     IADLHSEGFLSILNNVGYPNPNRSHFRSMDIWQSASDENKYLQTGWIGRWLDASCNGN
                     DIRPHLALEVDDTLSLALKGDKVTGLAMKNPSRLELILKDPLLQKIAGEWKDEEDHHH
                     NVEYLHKTLAEVSKSAGYLHDHLGKNMAKSVYPQHAFGKQMKVVADLILAGCETTVYY
                     VSLPGFDTHAGQNGQQNRMLKIYADGLKAFADDLKAAGQWDNTLVMTFSEFGRRVKQN
                     ASKGTDHGTANVMIFAGGSLKKKGILNEAPDLSDLDNGDLRFKVDFRQVYATVLQNWL
                     GTDAEIILGQKFGLLDFV"
BASE COUNT         3279 a         3584 c         3408 g         2691 t
ORIGIN      
        1 gtaaacccat aagttgaaaa accgaaaaca gttttcgtat gaaacgattt ttgctccacc
       61 tcgcgtttct cgtcacagcc gttgccggct gcaaagccca gccgccgacg cagctttcca
      121 cccggtcgaa gaaagcggag aaggcctaca acaaagcgct ggccgagctg aacgcttacc
      181 agtatgatct tgcgctggcc gatcttgccg tggcgaaaaa agcggacccg aatttcgtgg
      241 aagcgtattt tctcgaagcg aatctttaca tggaacagca ggaatggcag aaagcgatcg
      301 acgatttcaa aacaggtttt tccatcaacc cgaaatatta tccttcctgc tatttcgatt
      361 gcgcgaatgc ggagctgaaa ctgggacaat acgagggagc caaaacagat taccagaaat
      421 ttctcgacct gaagcgcaat gcatcgcagg atatgatcga caagacgaat ctcggcatca
      481 agtgctgcga cttcgccctt gacgccatgc agcatcctgt tccgttcaaa cctgtcaaca
      541 tgggaccgaa catcaattcg gactggtgcg aatattttcc gaacgtcacg gccgacgacc
      601 agacattcct cgttacacgc aaccagcaga agatcgatcc gaacaacggc gacaaaattt
      661 acacgcagga agatttttac atcagctaca aaaacgacag cggccagtgg agcttcatgc
      721 gcaacatggg tgcaccgatc aacacaccgg agaatgaagg cgcgcccagc ctttctcccg
      781 acggacgttt tttgttcttc gccggctgca tgggcgagaa cggttacggt tacgaccgtc
      841 gcggttacgg gagctgcgac attttctaca cgcagaaaat gaacggaagc tggacgcggc
      901 cggtgaatgt cggcgcgccg gtgaacacga aggaatggga atcgcagccg tcattctctt
      961 ccgacggcaa aacattgtat ttcgtcagca gccggggcgg cggttacggc gacagcgaca
     1021 tctggatgtc tacgctgaat gacgacggaa cgtggagcga gcctgtcaat ctcggcgacg
     1081 tcatcaacac accgggaaaa gaagaagcgg ttttcattca cccggacaac cagacgctgt
     1141 atttcgcttc cgacggccac gtgggaatgg gcggactcga cctttacgtc agtcaccgcg
     1201 attccgtaac gggaaaatgg ggcgagccgg tgaacctcgg ttacccgatc aacacgtacg
     1261 gtgacgaaag cgggctcatc gtcggcggca acgggcagat cgcgtatttc tcttccgacc
     1321 gcaaaggcgg gttgggctgc gacgacattt attccttcga gctcccgaaa gcgctgcagc
     1381 cttttcccgt gacgtacatg aaaggaaaaa ccttcaataa aaaaaccggc gctcctgtcg
     1441 gtgcgaaatt cgatctcatc gatcttgcaa cgggaaaaac attcatcacg tcgaagtcgg
     1501 atgcggcgaa cggggaattt ctcgtttgtc ttcccacgaa taaagattac gcgctgaacg
     1561 tgaacagtcc cggttttctt ttttactccg aaacattccg gctgaaagaa tcgcccgacc
     1621 cggacaagcc gttcatcatg aatgttccgc tgcagccgat cgatacaggc gcggtggtcg
     1681 agctgaagaa cgttttcttc gaaacaggga aattcgatct gcgccccgaa tcgaaagccg
     1741 agctcgacaa agccgttgcg tggctgctcg ccaacccgga cgtgaaagtg gagatcagcg
     1801 ggcacacgga taatgtcggc gataaaaaat cgaacatgac gctctccacg aaccgcgcga
     1861 aagccgttta cgattacctc gtcgcgcacg gcgttcccgc ttcgcgcatg acgtacaaag
     1921 gctacggcga cacgaaaccg aaagtcgaca atgacacacc ggaacatcgc cagatgaacc
     1981 ggcgggtgga gatgaagatc accgcgacga aataagcagg aacgggaatt cggctcatgc
     2041 gtatttttct ccgcgtcttc gcgtgagcct tcaatacaag aacgcgagaa ttgatccatg
     2101 aaggatcacg gcgcacaact tcgggtcctt tgtgcgcagc aaagaaccca tgggcttcca
     2161 ttctgcgctg cacataagcg cgtaacttta ctattacgct catgacaaaa tggtcgcaga
     2221 taaacgcctg gctgcattac cggaaaactt cggtgacaaa gcacggcgtg cattctcctt
     2281 ttgtgtttac gctggcgacg aaggtttttc ccggaccaaa gaacagcgat ccggaagagc
     2341 atcccgcgga agattaccgc acggagctga tcatgagcaa ccgcgtgatc gacgtgcagg
     2401 atttcggaac gggaatttca ggtccgcgga aagttgccgc catcgcaaaa cactcggcca
     2461 aatcgccgaa agagggacag ctgctgcacc ggatcgtgaa acatttcggt ccgaaaaaaa
     2521 tgctggagct gggcacttca ctgggcatta cgaccattta cgaagcgacg gcgacaccgt
     2581 ttgaaaaatt cattacgctc gaaggctgcc cgaacactgc gtcgctcgct gaagagatct
     2641 tccggaaaga aaatttacac gtggaagtgc gcacgggtga gttcggcacg acattgaaaa
     2701 atgcgctcga ttcactggaa aatgtcgact atgttttctt cgacggaaat caccgtgaag
     2761 aagcgacgct ggactatttc gaaacctgcc ttccctatgc gaacaacgat tccgtttttg
     2821 tgttcgacga cattcactgg tcgccggaaa tggaaaacgc ctgggaaaag atcaaaaccg
     2881 atccgcgcgt acgtttatcg atcgacgtgt tccatttcgg gctggttttt ttccgtgagg
     2941 aacagatgga gaaggaagac tttgtgctgc ggtattgaca tgccaggtgt gcgtaccagg
     3001 acgtgagcaa agaaattcac gccccggaaa acgaagcttg cctgcggcaa acccgttccg
     3061 gttacttaag cgggaagaaa ttttctaacc atccgctcgc ttttaccggg gagctttcac
     3121 aaagccatat ttttgaattt catccttacg gatagaaaca ccgggcctgg cacgccgcat
     3181 tccgttcgct gctcacgtct catgcccaag tcccaaattc cctaccttta acttcccgat
     3241 gaaaatcaac gctgcctggc atgaaaagaa taaaatgccg cggaacccga ccatcgaaga
     3301 aaggatccgc tggcatgtgg cccacgcgaa acactgcggt tgccggccca tcccggaaaa
     3361 gctgcaggaa gaaatgaaaa agagaaaaag aaaatgaacg gaaacaacca ggagatcatc
     3421 tcgctgtccg gtatttcacg ggagtaccgg gtgggaacgg aaatcattta tgcgctccgt
     3481 aatatatcgc tgaaaattta caagaacgaa tacgtggcgc tgatgggtcc ttccggttcc
     3541 gggaaatcga cgctgatgaa tgtgctcggc tgcctcgaca cgccatctca cggcgaatac
     3601 atcctgaacg gaacgtccgt cgaaaaaatg agcgacaacc agctggccga agtgcgcaac
     3661 aaggagatcg gtttcgtttt ccagaccttc aatctcctgc cccgctcttc cgcgctcgag
     3721 aacgtcatgc tcccgctcat ttatgcgggt ctttcaaaag cggaacgcca gcagaaaggc
     3781 gaatatgcgc tggagcaggt gggactgaaa gaccgcatgc accacaagcc gaacgaactt
     3841 tcgggcggac agcgccagcg cgtggcgatc gcgcgtgcgc tcgtgaacaa tcccgccatc
     3901 attctcgccg acgagcccac gggaaatctc gactcgaaaa cgtccgtcga gatcatgggg
     3961 ttgttcgaag cgatccacaa atcaggcaac acgatcatcg tcgttacgca cgaagaagac
     4021 attgccaaat acgcgcaccg cattgtgcgg atcaaggacg ggctggtgga aaacgatcag
     4081 ctgaacaacg acatccgcca ggtgaacgct cccgcatttt ccgatcactg acgccgttct
     4141 ttcttcggga acaggcgcta cccaaatttc catcccatga cgataaagat ctacacgaaa
     4201 accggcgaca agggacaaac gtctctcatc ggcggcacgc gcgttcccaa gcaccacatc
     4261 cgcatcgaag cgtacggcac cgttgacgag ctgaattcct ggatcgggtt gctgcgcgac
     4321 atgacgcagg atgcgcacgt gaaggaaatt cttctcgaaa cgcaggaccg tcttttcacg
     4381 gtcggctcgc tgctcgcttc cgacccggaa aaaagcaaaa tgaaattgcc cgaaatacag
     4441 gacagcgata tcacgctgct tgaaaaagag atcgacgcga tggaccagtt gcttccgccc
     4501 atgaaaagtt ttattcttcc cggcgggcac gttaccgttt cgcattgcca cgtcgcacgc
     4561 tgcgtatgcc gccgcgcaga acgtaacatc acccaccttt ccgaaacagc gcccgtggac
     4621 gagctcatcg tccgctacct caaccgcctt tccgattacc tcttcatgct ttcccgcaag
     4681 ctgtcgcatg atctcggcgc agaagaaatt ccctggaagg cgagaatgtg aacaggacgt
     4741 tgcgaaggcc gcttcttttc gcactctgaa gaccgtgcca aaccttcatt ttgttgaaat
     4801 gttggcaaat cactgattgt cagaatattt aatcttcata aaaaacatcc cctctccttt
     4861 gatccgatac tgatttttat acttttgcac gaaaattgaa tcctgcgcag gttgaaaaac
     4921 cagcgtaaca ttttaaagga ttccgcgttt taaatcaata ataaataagg aggaatttat
     4981 gtactggacc ctcgaacttg cttcttacct cgaagacgcc ccctggcctg ccacaaaaga
     5041 agagcttatc gattacgcga tccgctccgg agcgccaatg gaagtgatcg aaaacctgca
     5101 ggagatcgaa gacgaaggcg aagcctatga atcaatcgaa gagatctggc cggactaccc
     5161 gaccaaagag gatttcttct tcaatgaaga cgagtattga tcgcagatag cttgaacagt
     5221 ttgaactcag ggggacttcg gtccccttgt ttattgggta acggcccggg aacgggcatc
     5281 cgcctgacaa tgcggcgcag atttatcaca tttggcagat ttctccttgg ggaatgaatt
     5341 ttgatctgta atttcacgat ccgttttcca tgatgaaaaa ccgtcaccgg cacgctctcc
     5401 cgcttcttgc aacacttgca ggatgcgtgt tcatgcttgc ctgggtcgat ccggcacatg
     5461 ccacggaggt tacacgccag cttacacaga aagggtttgt catttcccag ctgcgtaaat
     5521 cggaagtggc gatcgtcagg aattccggcg cacagctcaa agtcatgttc cggggattcg
     5581 attccccctg cgcaggtccc tgcacaaccg tgacggagct tcttccccgc aatacgcatc
     5641 attatctttt catcttcccc cgtttcgtga aattatcgct gcaggacggt atccgtacct
     5701 ggatgaaacg cgaatgagct ttcccgggat gagctttcat tccccggcgg ccacgcgccg
     5761 tattcccttt ttttcagtct acctcaatct gcagttccgg gataagctgc gccaatctct
     5821 ttaagcaacc aatcatgttt gatttcataa gcaaaggtat ttcccgtctt gtgggcggtt
     5881 ccaaatcgga acgcgatgta aagcagatca tgcccatcat cggggagatc aacgaacatt
     5941 tcgagagctt caaatcgctc tcccacgacg agctccgcaa caaaacgaaa gaattccgca
     6001 gccgcatcgc tgaatacatc agcgaagaag agaacgagat ccgcgagctg cgcgccaaat
     6061 ccgaatcgcc cgatatttcc atcgacgagc aggaagatat tttcaacagg atcgacgaga
     6121 tcgaaaaaac gatcctgaaa aagatcgaag aagtgctcgc ggaaatccag gctgaagctt
     6181 ttgccgtggt gaaggaaacc gcgcgtcgct tcatggaaaa taccgagatc gtcgtcaccg
     6241 ccaccgacca cgaccgcaac ctttcggtga aaaaaccgaa tgtcgtcatc gacggcgaca
     6301 aggcgatctg gaaaaacacg tggatcgctg ccgggaacga agtgacgtgg aacatgctgc
     6361 attacgacgt gcagctgatc ggcggaacgg tgctccacca gggaaaaatt tccgagatgg
     6421 ctaccggtga aggaaaaacc ctcgtcgcaa cgctcccggt ttacctcaat gcgctcccgg
     6481 gacgcggcgt ccacctcgtt acggtcaaca actacctcgc acgacgcgac gccgaatgga
     6541 acgggccgct gttcgaattc ctcggtctca cggtcgactg catcgacctg cacgagccga
     6601 actcgcagga acggcgcaac gcgtacctcg ccgacatcac gtacggcacc aacaacgaat
     6661 acggcttcga ttacctccgc gacaacatgg cgcggcagcc ggaagagctt gtgcagcgca
     6721 agcaccatta cgccatcgtg gatgaggtcg actccgtgct gatcgacgat gcacgtacac
     6781 cgctcatcat cgccggacca acgccgcgca acaacaacca catgttcacc gagctgaaac
     6841 cgaaagtgga aaagctcgtc gccgcgcaga agacgttcat caacaccatc cttgccgata
     6901 cgaaacgtct cttcggcgaa gccgcgaaaa gcaaagagaa agaaaaagaa gcgggactgc
     6961 tcctgctccg ctgcttccgc ggtctcccga aaaacaaagc gctcatcaaa ttcctgagcg
     7021 aacccggcgt taaagcgctg ctgcagaaaa cggaaggcgt ttacctgcag gacaaccagc
     7081 gcgagatgca caaagtggac gaagcgcttt atttcgtcat cgatgaaaag aacaatacga
     7141 tcgagctgac ggaaaaaggc atcgatctcc tttccacatc ggcggaagat cacagcttct
     7201 tcattctccc cgacctcgcg ctcgatcttt ccgagctgga aaaaacctac ccggatgaaa
     7261 aagaaaggct cacgaaaaaa gaagagctca tccgcgatta caacgtcaag tcggaacgcg
     7321 ttcactccat gcagcagctc ctgaaagcgt attgcctttt cgaacgcgac gacgaataca
     7381 tcgtcgccga cgggaaagtg aagatcgtgg acgagcagac gggacgcgtg ctggaaggac
     7441 gccgctattc cgacgggctt caccaggcga tcgaggcgaa agagaacgtg aagatcgaag
     7501 ccgccacgca gacgtacgcg acgatcacgc tgcagaatta tttccgcatg taccacaagc
     7561 tctcgggcat gacgggaaca gcggaaaccg aagcgcagga attctggacg atctacaaac
     7621 tcgacgtcgt tacgatcccg acgaaccgcc cgatcacgcg gaaagacctc gaagatcttg
     7681 tttacaaaac caagcgcgaa aaatacaacg cggtcatcga cgagatcgtg aaatgcgtgg
     7741 aagcgggacg accggtcctc gtgggcacaa cgtccgtcga aatttccgaa ttgctcagcc
     7801 gcatgctgaa gctgcgcaac atcaagcaca acgtactcaa cgcgaaactg caccagaaag
     7861 aagccgacat cgttgcggaa gccggtaaag ccggaaccgt gacgatcgcc acgaacatgg
     7921 ccggccgcgg aaccgacatc aagctcggac ccggcgtgaa ggaagccggc ggtctcgcca
     7981 tcatcggaac ggagcgccac gaatcgcggc gcgtcgaccg gcagctgcgc ggacgcgcgg
     8041 gacgccaggg cgatcccgga tcgtcgcagt tcttcgtttc gctcgaagac gatctcatgc
     8101 gcctgttcgg ctccgaccgc atcgcgcgtc tcatggaccg catggggctc gaagaaggcg
     8161 aagtgatcca gcacagcatg atcacgaaat ccatcgaacg tgcacagcgg aaagtcgaag
     8221 aaaaccattt cggcacgcgt aaacgcctga tcgaatacga cgacgtgatg aatgcgcagc
     8281 gcgaggtgat ctacaagcgc cgccgccacg cgctgttcgg tgaacgcctg cagatcgaca
     8341 tcagcaacat gatccacgac gtaacggaaa acatcgtcgt gaacaaccac gaatcggaaa
     8401 attacccgca gttcgagctc gacatgctgc gcgtcttcgg catggaagcg ccggtgaagg
     8461 aagaggattt caaagccgca cgtcccgcgg agctcgcccc gcaggtgacg gataccgttt
     8521 ccgcttttta cagggaaaaa tcggcgaaca tggccgcacg ttcgttcccg gtgatcaaaa
     8581 atgttttcga aacgcagagc gcgaattacg agaatatcgt tacaccggtc accgacggca
     8641 tacgcacgct gcaggtcatc gccaacctta agcgctgcta tgaaacgcag ggtcgggagc
     8701 ttgtcttcac gttcgagaaa gcggccatcc tcgcgatgat cgacgatgca tggaaagaac
     8761 acctgcgtga aatggacgac ctgaaaacga gcgtgcagaa cgccgtttac gaacagaaag
     8821 atccgctgct catttacaag ttcgaatcgt tcgatctttt caagcgcatg gtggacgaaa
     8881 cgaaccgcga cgtcatttcc ttcctgttca aaggaaatct tcccggcaac gaaaacgaac
     8941 agcgcataca ggaagcgcgt ccgcgcgcac cgcagccgca accgaagctg cagaccatcc
     9001 acgaagaagc cgggggaggt tcccagcagc aggcacagca gcagcaggga cgtccagcga
     9061 tgccgcagca ggcgccgcag cccgtgcagc aggtcatccg ccaggatccg aagatcggcc
     9121 gcaatgatcc gtgtccgtgc ggaagcggga agaaatacaa gaattgtcac gggaaataac
     9181 tgtgagccat tagaaaaaga tccgctgtaa caggcggatt tttttgtgcg ggttcgaatg
     9241 gcgtcgcctg aaagcctacc ttgaaccgta ttaccaaaac tccggcggtg aacaggcgac
     9301 acttcctcag gcagtctgca ttgatttcag cgggggcgct tttgcttccg ccggcgctgc
     9361 tcgaggcgtg ccggaaaaaa tcagcggatg aagacattgg cttcggcgga aaagtgctga
     9421 tcatcggtgc gggtgcggcg ggtctctatg cgggatacat cctccgctcg aaaggaatcg
     9481 attttgaaat cctggaagcg tcttccgctt acggcgggcg tgtcggaaaa ataaacggct
     9541 tcgcggattt cccgctcgac acgggcgcac aatggctgca cgggaagaac aacatcctcg
     9601 gtgatctcat cgcaaaaaca cagacggaga tcacgctcga cgattccgcc gaattcttct
     9661 ggttcaacaa ccagatcacc ggtacgctgc cggacgacat cgaagctatc ttcctgcgcg
     9721 aagacaacgt tcctgatgtt tcgttcaaag acttcgccgt gcaggaaggt ttcggaaatg
     9781 aatatgcgga tatcgtggag ggcatagcgg gcgactcggg tgcagctgca tcacggatct
     9841 ccgcttactg gaaaatacag gaagaagcag cgtggaattc cggcgacgat gatttcaaat
     9901 tccgcgggac gtatttcgat tttatcgata cgaacatcgc cgcgcaggtg aaagaccgga
     9961 tacagctgaa cacgatcgtt tcgaagatcg attattcgca aagctccgtt gtcgtgacgg
    10021 acagcaacaa caacacgtac accgccgaca aagtcatcgt taccgttccc gtcacgatcc
    10081 tgaaagccgg cgacatcgaa ttcgttcccg cattgacgca ggaaaaaacg gatgcattcg
    10141 cgaagatcgg gatggacgcc gggatgaaag tcttcctgaa attttcctct gcgttctatc
    10201 accagaacat catcggcgga aacacgtgcg gcgcttacgc ggatgagatc atcggcaaaa
    10261 ccggttccga tcacgtgctg cttgcgttca tcatggggga gcaggcggaa cgtttaacag
    10321 cgctcggaag cgacagcgcg atcacttcgg ccctgctgca ggaactcgat acgatgtaca
    10381 gcgggcaggc gaccgcttcc ttcatcggtt cgcaggtcat caactggaca acgcacccgt
    10441 tcatccgcgg cgcgtattcg tacagcaccg tcggcatggg aaatgcgcgg gaaaccgcgg
    10501 cgcaacccgt ggacaagaaa atctttttcg ccggcgaagc gatgaattac aacgggaacc
    10561 accagactgt tttcggcgcc gtggaaaccg gttatcgtga agtgatcaac attttaaaca
    10621 gcgtgaagaa atgaaaaagc tgatcgctgc tttcgtgctg ctgcttttct gccgctgttc
    10681 cttcgccctg gaaaacaagc tggaactatc gtacacgtac ctgtattccg ggcaatggga
    10741 caaagcgata caggcgtaca atttcagtcg cccgttcctc gcaaaccagc aaccactatt
    10801 catgaacggg ttgaatgcat cggcgtcaca cattttcaaa ccgcacaagg tattcagcta
    10861 tggaataacc gtttcgtacc tgtatttccg gagcagcgtg aaaaacgaga atttcctcaa
    10921 cacgctgaac ctccatttcg cggacatagg ctggcttgta cattttgaaa acacggaaaa
    10981 gccggatggc ttctattccg agcttgcgtt ttccgccgtt tcttccggac tgttccggac
    11041 tgtcaaccgg aaatcattcc ggtacgacgg cgcgcgcgcg aaagcgttcg gtattggcgg
    11101 tgatatccat cttaaagccg ggtatgaatt cagcgcgaag aacaactttt tcatttcacc
    11161 tttcgcagcc ctcggttaca cgccgttcct tttttcaccg aatacggaag gcgtgatcaa
    11221 ccagacgaca ggattgaccg gcaggcggtg gacaggaatt gtgcaggtac aggcgggaat
    11281 tgcgctgcac tggaagaagg agacgtaaga cgaccggtgc gtttttttgt gcgggttcac
    11341 cccgttttca gaagcgcaac aactgtgcgt ttcattcact ttgttcccgt gctcggcagc
    11401 tgcactgcac ttgcctcgcc cggggctgga gcggtttctt ccgtgcttct ctttcgctct
    11461 ctgttttttg aagaacacct gcgatcgccg gatgaaaaaa ctgccgggcg ataaaagctt
    11521 tacaacaact tcatcgttcg tttcccggaa tcctctcaca caaaatccag cagcccgaac
    11581 ttctgcccga ggatgatctc cgcatccgtg ccgagccagt tctgcaaaac cgtcgcgtac
    11641 acctgccgga aatcgacttt gaacctgaga tcgccgttat cgagatcgga aagatcgggc
    11701 gcttcgttca gtatcccttt tttcttcagg ctgccgccgg cgaagatcat cacgttcgca
    11761 gtaccgtgat cggttccctt gcttgcgttc tgcttcacgc gcctgccgaa ttccgagaac
    11821 gtcatcacga gcgtattgtc ccactgcccc gctgctttca ggtcgtcggc gaatgctttc
    11881 agtccgtcgg cgtaaatttt cagcatccgg ttctgctggc cgttctgccc cgcgtgcgtg
    11941 tcgaacccgg gcagcgaaac ataataaacg gtggtttcgc aaccggcgag aataagatcg
    12001 gcgaccacct tcatctgttt tccgaaggcg tgctgcgggt aaacggattt cgccatattt
    12061 ttcccgagat ggtcgtgcag gtaaccggcg ctcttcgaaa cttccgcgag cgttttgtgc
    12121 aggtactcga cgttgtgatg atggtcttcc tcgtccttcc attcgcccgc gatcttctgc
    12181 agcagcggat ctttgaggat gagctcgagg cgcgaaggat ttttcatcgc gagccccgtg
    12241 actttatctc ctttcagcgc gaggctcagc gtgtcgtcga cttccagcgc gaggtgcggg
    12301 cgaatgtcgt tcccgttgca gctcgcatcg agccagcggc cgatccatcc cgtctgcagg
    12361 tatttgtttt catccgacgc gctctgccag atgtccatcg aacggaaatg cgaacggttg
    12421 ggattcgggt aaccgacgtt gttgagaatg gacaaaaatc cctcgctgtg aaggtcggcg
    12481 attccctgca gcgcgccgtt gaacgagaaa tgatcgtcga tgcggatgac ctggttttca
    12541 ttgaggccga tggacgggcg gttcgaatga tagaggtcat cgccgaaagg aatgatcgtg
    12601 ttgagcccgt cgttgccgcc ggaaagctgg atcacgacaa tgcgttttcc attggtgccg
    12661 gagagaaaaa gctgcttcat catttcttcg ctctgcgcat cgttgccgcg caggaatttc
    12721 ggcagcagga acgaagccga ggccagtccg gccagcttca ggaaatcttt tctcgagtag
    12781 gtgctcatgt tctttgtttt tttgatccgg tgaatccgca gcagctgatc ccgttttttc
    12841 cgttcctggt ttttcagctt acacgatctg gaattcgggc gtctgcatca ccgcgacgat
    12901 cgtcgtaatg atcttccgct gcttcgtaga agtatccacc tgcgcttcga tgagcttgcg
    12961 gt
//
LOCUS       DASTSF010000408        10811 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1436059,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000408 DASTSF010000000
VERSION     DASTSF010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 10811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10811
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1436059"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..759
                     /locus_tag="VFU15_11735"
     CDS             <1..759
                     /locus_tag="VFU15_11735"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HEU4718502.1"
                     /translation="DTASVTVNIIPLPVASVSGTTMICQGDSAELNAGGGGSYAWNTG
                     ATSSTIYVSASGNYFVIVSNSCGSDTAQINVAVNSVNALFSADTLSGSPPLTVNFTDN
                     SSPGTVSWQWNFGDNTNGSGSSVTHVYTDPGSYTVTLTVTDANGCSDTYSVTILVNDV
                     PSWVSVPNIFTPNGDGTNDVFLVNSAGLKEMKLEVYDRWGVLVNTISTPAMGWDGRTT
                     SGVQATDGTYYYVLNATGYDNKNYSMTGFITLLR"
     gene            913..2214
                     /locus_tag="VFU15_11740"
     CDS             913..2214
                     /locus_tag="VFU15_11740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718503.1"
                     /translation="MVKQIFLAGNLVLALNAAGQATFNASAVYTQNFGTSTVATWTDN
                     STFPGWYALSWKVAAAGTDPFRGTTNITAAAPVNSGGWYVYQCNGGTDMKLGTRPSNS
                     SGGPDSPGMDGRNGIALGLCLQNNLPTINSLVISFDSYQLSLADEGGSANTVFFDYLV
                     SAGIPALSAAGYTNVATLNFTPPQSSGIAGSAQINGYPCPGNCQTVNKSACITVNIPS
                     GSYIMLRWWDPNDGSNDPHVAIDNVSVTAYSDNLCATVLPIELLGFNAKQEGSKVTLN
                     WSTATETNNDFFTVERAGSDEDFHPVATVSGNGTTTVQHSYSAEDLSPENGINYYRLR
                     QTDYNGQSTVSDIVTVNFSSDADDFSPGVSLCGQDLCYSAGAWNASIRMTVYDMTGRK
                     LAEQDIQPGENGSVPLPANYQGMLIVSFVSEDEVRVVKIIP"
     gene            2243..2374
                     /locus_tag="VFU15_11745"
     CDS             2243..2374
                     /locus_tag="VFU15_11745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718504.1"
                     /translation="MKRKLVHIFRRDDDKLVHVVTLYVTLIFWLVTVAVIFLVQNAG"
     gene            2430..2834
                     /locus_tag="VFU15_11750"
     CDS             2430..2834
                     /locus_tag="VFU15_11750"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU4718505.1"
                     /translation="MIAWFEIPASDFEKTVSFYSRILETPVEVHVFNNQRHGIFVHRP
                     GQIGGSIVESEPAEKRNGPVLFFRVRDDISSVLDRVEACGGKIIMPRTLIRNIGEDKR
                     SVIPRNLIDQKIGYYAVFEDPEGNRMALYTSH"
     gene            2984..5302
                     /locus_tag="VFU15_11755"
     CDS             2984..5302
                     /locus_tag="VFU15_11755"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEU4718506.1"
                     /translation="MLNLRKIILFSAFLVPLLLPAQQKPNMDNYQPPQSNGPLPPNFL
                     ISTVDKYEQDRQKIQQNQDKKMQKAEDDFYLQTNYSVDQMCFSGEVLVNDTMGQYVNR
                     VADTLLWNDKETRKNISFYILRSPIVNAFTTDQGIIFVTVGLLTRLDNEAELAFVLGH
                     EITHYKHHHVYIGYVEGVKMQQGMGQYEATTFENRYLKRHRYARSQESQADDEGFDMM
                     VAAGYDPHAAIKAFDILALADYPFSDTTFNKSFFETNYLIFPSKYQPDTVKGIRPDNE
                     DEDDDLATHPSTYKRRKAIIHKFNKLQDTTGALFKVSESMFWRVKAIARFEECSEHSQ
                     DQEFSEAMYSDYVMQSSYPDNYYLEKEMVRAMYASVIEKNKTLNLDDLMALFQSLFII
                     GGPDDRPVGEQGRLKGFVSKADATGWNIAALHYAWDVHKKYPDDPDISYWCTGLFREL
                     TLKNKVRIGEFEKNDTEFVHFGNRILKDTAIVNHSKIATPAERYQLALDHMSKDSIDT
                     YHYWQFAFIDDLKDSAFVAQFKEATVWADSVHLQDSLWYMQSTRAQDKSKGEIESDLS
                     GPQGITKLVAVNPVYIAYIDHGDDGDQEVDVRNSLDGHSLLVDEMKKDAKMFNLDLTL
                     LDESGLDTSSVDRFNDLSVASQWFAQRDNYTDHQVLPYSQEKMKALAQKYGTPYFMWS
                     AYITYKGKRRGNLWRALSFLVTPLAPHIAYRMLTPHEDVYYIAIIYDVNTGYPVFVQK
                     TQMSNQHENKSRLRLHVFDLFHQITEPKKKKK"
     gene            5304..5960
                     /locus_tag="VFU15_11760"
     CDS             5304..5960
                     /locus_tag="VFU15_11760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718507.1"
                     /translation="MKKAALIFASVLAFHFANAGPHGGYIGHRIILAGEGAYSPFFTS
                     VKDFYTKYNFQYGGNLNVIVARRTQVGVFYNMWSLGGNQAFQSNMGKNDRVNGTQYGL
                     VVRNFRAGRGGLAPIGKFWDVSMSYASNTYKAGSDNKDILGGTPENVPVSSNQILAHV
                     AFGTQMVFWNHFVGNTGVRFGGPIYTIDKNNGGYKNFFAQRIAYKEFFSVFFGIGFLI
                     "
     gene            complement(6051..8405)
                     /locus_tag="VFU15_11765"
     CDS             complement(6051..8405)
                     /locus_tag="VFU15_11765"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEU4718508.1"
                     /translation="MRRRLPGLFFIFFAFPAFAQHAPLEPKGPMPAYFQCPVYQQVDK
                     DIARRQAQGDVVSDQEKDFMYESSYFMQQRMFSGYVLYNDSISDYISSVGAEVLKDDT
                     ATLHKLHFYAYKDADPNAYTSSTGTVLITLGLLAQLENEAQLAFILCHEITHFRQQHM
                     LKGYLNREELKNQKGKTPMYLLQSSYYSYNQEQELEADRLGFELFMKSGYAKKEALRS
                     FDVLEYADLPFDDVPFDTMFFNKDYMKIPPGYFMKEVDPIYTDDNYDDLGSTHPNVRK
                     RRMALMTTLDTVQNKDGSLFMVSKDNFLASRENARYEICRLYLQDRNYPAAVYCSYMM
                     LQRHPGDSYLEKIVGESLYEIAAYYQSGKKTGFMIYDFWGFFGTYGGKYDALNRSGYY
                     HLPDFKDNPGQQEQIFHLFHEIEPDEMTVLALSYNWTLHKKNPSDSLLSTLCDSLFSM
                     LVNNQNLHLSYFSTVTPDEAKAQLQKDSLSRVQETGETGDSKFSRLDKFKLNSGKERF
                     TKFAFVEMLKDTEFVSRFKYYTDNRRSLVYTPDYSTYDFRTKKERKADEKEEKNYGFG
                     ITKIIVAGPDYEYYKQKNRNDDPDMDYRMSEDGQTRMTGVIKAQASAAGVNAIILNPM
                     TMDSTAGDSLSDLAVLNEWFYEKLQHGNHSWAWTVNDQSRADSIAKKYGTRYVMFTSV
                     EADYYRKIQHPVLFAMSCAFVVPAVRAFIPREKFFLDAAILDLKTGEVINVKHQLLRK
                     GKEGNRTTEFYRDLFSKMVKPKREKPANTHGRGGKPVDEDERGM"
     gene            complement(8449..10281)
                     /locus_tag="VFU15_11770"
     CDS             complement(8449..10281)
                     /locus_tag="VFU15_11770"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016195841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEU4718509.1"
                     /translation="MGYSSLRDCIGDLERNGHLVRVKEMIDPDLEMAAVHLRVHEAGG
                     PALLFENVKGCAFPAASNLFGTKERSRFIFRDAIENVKALIRLKSDPSEALRHPFRNW
                     KTGWAALKSFPKKTSALAVLENEIRLHDLPQIRHWPDDGGAFVTLPAVYTEDPEFPGV
                     MHSNLGMYRIQLNGNEYVRDREAGLHYQLHRGIGVHQSKWNAKGLPMKVSVFVGGPPS
                     LTFSAVMPLPEGISEMTFAGVLGNRKIRYGYRDGFCISGDADFVITGEVHPGENKPEG
                     PFGDHFGYYSLTHPFPVMRIHKVWHRKNAIWPFTVVGRPPQEDTSFGWLVHELAGKGI
                     QDEIPGLKQVHAVDAAGVHPLLLATGGERYTPYLKEKRPSELLTIASHILGTGQLSLA
                     KYLFIAEETGEKNPDAENIPVFFQHVLERIDLSRDVHFHTNTSIDTLDYSGDGLNTGS
                     KVVFAACGEKKRTLSSHLLTELPGDFHSARLVFPGVTAISGPRFSSAEKAKQEIAQLC
                     GFLAERKPQTEGLMIIVVCDDSQFTAHDTASWLWVTFTKSNPARDIYGVNDFYENKHW
                     GCRGPLIIDARTKTHHAPPLEKDPTIETRIDRFFVAGGSLHGIA"
     gene            10350..10640
                     /locus_tag="VFU15_11775"
     CDS             10350..10640
                     /locus_tag="VFU15_11775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718510.1"
                     /translation="MKKPICLRFASLILFLFSTLVLSALDEVDSLERLLKQEAPDSVR
                     IDRLMTLSKIFMYSGGEASLKNADAAMRLAHSSGDRSREMQAYATRGKTFLL"
BASE COUNT         2546 a         2824 c         2913 g         2528 t
ORIGIN      
        1 gatactgcgt cggttacggt gaatattatc ccgttacccg ttgccagcgt aagcggaacg
       61 acgatgatct gccagggtga ttccgctgag ctgaatgcag gcggtggcgg atcatacgcc
      121 tggaacacgg gagcgacttc ttccacgatc tacgtttctg cttccggaaa ttattttgtg
      181 atcgtctcga acagctgcgg atcggatact gcgcagatca acgttgcggt caattccgtc
      241 aatgcgcttt tttctgccga cacgcttagc ggaagtccgc cgctgaccgt gaattttacc
      301 gacaacagct cacccggaac cgtttcctgg caatggaatt tcggtgacaa cacgaatggt
      361 tccggatctt ccgtgacgca tgtctacacc gatccgggca gctacacggt gacgctgacg
      421 gtaacggatg cgaacggttg ttcggatacc tattcagtga cgatcctggt gaatgatgtt
      481 ccttcgtggg tttccgttcc gaatattttc acaccgaacg gagacggaac gaatgatgtt
      541 ttcctcgtaa attccgcagg attgaaagag atgaagctcg aagtttacga ccgctggggc
      601 gtactggtca atacaatttc gacaccggcg atgggatggg acggaagaac aacttccggc
      661 gtgcaggcaa ccgatggaac ttattattac gtgctgaatg caaccggtta cgataacaaa
      721 aactattcaa tgaccggttt catcacattg ctgcgctaat aaataaaaca cgcataacga
      781 tggtgtaacg gtcgcggaat aaattagcat ccgttcaccc gaacgatttt tcaggacgaa
      841 gtgaaaatcc ttgaggaata aatgcacaat gcatttatcg tccgaaaccg cttcccgcaa
      901 accagctccc ccatggtgaa gcaaattttt cttgccggga atttagtatt ggcgctgaac
      961 gccgccgggc aggccacatt caatgcatcg gcggtttaca cgcagaattt cggaacgagc
     1021 accgtagcga cgtggacgga caattccact tttcccggat ggtacgcgct ctcctggaaa
     1081 gtggcggcgg caggaactga tccgttccgg ggaacgacga atattacagc tgccgcaccc
     1141 gtgaacagcg gcgggtggta cgtgtatcaa tgcaatggcg gaaccgacat gaagctcggc
     1201 acgcgtccct cgaattccag cggcggaccc gacagccccg gcatggacgg aaggaacggc
     1261 attgcgctcg ggttgtgcct gcagaataat cttcccacga tcaacagtct cgtcatcagc
     1321 ttcgattctt accagctctc gcttgcagac gaaggcggtt cggcgaacac cgtgtttttc
     1381 gattacctcg tgagcgcagg gataccggcg ctgagcgcag caggttatac gaatgttgcc
     1441 acactcaatt ttactccgcc gcagtccagc ggaattgcag gcagcgcgca gatcaacggt
     1501 tacccgtgcc cgggaaactg ccagactgta aataaatcag cctgcatcac cgtgaatatt
     1561 cccagcggtt catacatcat gctgcgctgg tgggacccga acgatggcag caacgatccg
     1621 cacgtggcga tcgacaacgt ttccgttacc gcgtattcgg ataatctgtg cgccacggtt
     1681 cttcccatcg agcttctcgg ttttaatgcg aagcaggagg gaagcaaggt gacgcttaac
     1741 tggtcgacgg caaccgaaac gaataatgat ttctttacgg ttgagcgcgc aggcagtgac
     1801 gaagattttc atcccgttgc gacggtaagc gggaacggca cgaccactgt tcagcattcc
     1861 tattccgcgg aagatctttc cccggagaac ggcatcaatt attaccgcct gcgccagacg
     1921 gattacaacg ggcagtcgac agtgagcgat atcgtcaccg tgaacttcag cagtgacgcg
     1981 gatgatttca gtccgggtgt ttcgttgtgc gggcaggacc tttgctattc ggcgggcgca
     2041 tggaatgcat cgatacggat gacggtttat gacatgacag gcaggaaact cgcggagcag
     2101 gatattcagc cgggtgaaaa cggctcggtt ccattgccgg caaattacca gggaatgctg
     2161 atcgtttctt tcgtttcaga agacgaggtc cgcgttgtga aaattattcc atagaacaga
     2221 caacgtaaat agctgcataa tcatgaaaag aaagctggtg catattttca gaagggacga
     2281 cgacaagctc gtgcacgtcg tgacgctcta tgtgacgctg atcttctggc tggtgacggt
     2341 tgccgttatt ttcctcgtgc agaacgccgg ctgaaagctg caatggcaaa aacatttaat
     2401 actttcctgc aacgaacaac ttccattcta tgattgcctg gttcgaaatt cccgcctccg
     2461 attttgaaaa aacagtcagc ttttacagcc gcattctcga aacgcctgtt gaagtgcatg
     2521 tgttcaataa ccagcggcac ggaatttttg tacacaggcc gggacagatc ggcggttcca
     2581 tcgtagagtc ggaacccgcg gaaaaacgta acggcccggt gctttttttt cgtgtgcgcg
     2641 acgatatttc ttccgtgctc gacagggttg aagcctgcgg cggaaagatc atcatgcccc
     2701 ggacgctcat ccgcaatatc ggtgaagaca aacgttcggt cattccccgg aacctgatag
     2761 accagaaaat cggctattac gcggttttcg aagacccgga aggcaaccgc atggcgctgt
     2821 atacgagtca ctgaaaaagg ttctccgctg gcagagggag gacacggaag accggcggac
     2881 aggctttccg ccccggaaat gacaggaatt tcaccggtaa tttggatttt ttaggaggca
     2941 gcttattgtt aactgcggtt cccgccttaa ctttgataac actatgctta acctccggaa
     3001 aattattctt ttttcggctt ttctcgtgcc tttacttctt ccggcacagc agaagccgaa
     3061 catggacaac taccagcctc cgcaatcgaa cggaccgctg ccgcccaact ttctcatttc
     3121 tacggtcgac aaatacgagc aggaccgcca gaagatccag cagaaccagg acaagaaaat
     3181 gcagaaggcg gaagatgact tttatctcca gacgaattat tccgtcgacc agatgtgctt
     3241 cagcggcgag gtgctcgtca atgacacgat gggccagtac gtgaaccggg tcgcggatac
     3301 gctgctctgg aacgacaagg aaacacgcaa gaatatttcg ttctacattc ttcgttcacc
     3361 gatcgtgaat gcattcacca ccgaccaggg catcattttc gttacggtgg gactgctcac
     3421 gcgcctcgac aatgaagccg agctggcatt cgtgctgggt cacgagatca cgcactacaa
     3481 gcaccatcac gtttacatcg gttacgtgga aggcgtgaaa atgcagcagg ggatggggca
     3541 gtacgaagcg accacgttcg agaaccgcta tctgaagcgc caccgctatg cgcgcagcca
     3601 ggaatcacag gcggatgatg aaggattcga catgatggtg gccgccggct acgatccgca
     3661 cgcggcgatc aaggcgttcg atattcttgc gctcgccgat tacccgttca gcgataccac
     3721 gttcaacaag tcgtttttcg aaacgaatta cctcattttc ccttcgaaat accagccgga
     3781 tacggtgaaa ggcattcgtc ccgacaatga agacgaggac gatgatcttg ccacgcaccc
     3841 gagcacctac aagcgccgca aggccatcat ccacaagttc aacaagctcc aggatactac
     3901 aggcgcgctg ttcaaagttt cggaaagcat gttctggcgt gtgaaagcga tcgcgcgttt
     3961 cgaggaatgc tcggagcaca gccaggacca ggaattttcg gaagcgatgt attcggacta
     4021 cgtgatgcag agttcgtacc cggacaatta ttacctcgaa aaagaaatgg tgcgtgcgat
     4081 gtacgcgagc gtgatcgaaa agaacaaaac gctgaacctc gacgacctga tggcgctctt
     4141 ccagagcctg ttcatcatcg gcggtccgga tgaccggcct gtgggcgagc agggaaggct
     4201 caaaggattt gtttccaagg cggatgcgac aggatggaat atcgcagcgc ttcattacgc
     4261 gtgggacgtt cacaaaaaat atcccgatga tccggacatt tcatactggt gcacgggact
     4321 tttccgcgag ctgacactga aaaacaaagt gcgtatcggc gaattcgaaa agaacgacac
     4381 ggaattcgtc cacttcggca accgcatact gaaagacacg gccatcgtca accattcgaa
     4441 gatcgcgaca cccgcggaac gttaccagct ggcgctggat cacatgagca aagattccat
     4501 cgatacgtac cactactggc agtttgcctt catcgatgat ctgaaggatt ccgctttcgt
     4561 ggcgcagttc aaggaagcga cggtttgggc ggacagcgtg cacctgcagg attctctctg
     4621 gtacatgcag tcgacgcgcg cgcaggacaa gtcgaaaggc gaaattgaaa gcgacctgtc
     4681 ggggccgcag ggaatcacca agcttgtggc ggtgaatccc gtttacattg cgtacatcga
     4741 tcacggcgat gacggcgacc aggaagtgga tgtccgtaac tcgctcgacg gccattcgct
     4801 gctcgtcgat gaaatgaaaa aggacgcgaa gatgttcaac ctcgatctta cgctgctcga
     4861 tgaaagcggg cttgatacca gcagcgtgga ccgcttcaat gatctcagcg tcgccagcca
     4921 gtggttcgcg cagcgcgaca actacaccga tcaccaggtg cttccgtatt cacaggaaaa
     4981 aatgaaggcg ctcgcgcaga aatacggcac gccgtacttc atgtggtcgg cgtacatcac
     5041 ttacaaaggg aaacgccgcg gcaatctctg gcgcgcactg agcttcctgg taacaccgct
     5101 ggctccgcat atcgcgtacc gaatgcttac gccgcacgaa gatgtttatt acatcgcgat
     5161 catttacgac gtgaacacag gctatcctgt tttcgtgcag aagacgcaga tgtcgaacca
     5221 gcacgagaac aaatcgcgcc tgcgcctgca tgtgttcgat cttttccacc agatcaccga
     5281 accgaagaaa aagaagaaat aacatgaaga aggcagctct gatttttgca tcggttctcg
     5341 catttcattt tgcaaatgca ggccctcacg gcgggtatat cggtcaccgc atcatcctcg
     5401 cgggtgaagg cgcatattcg cctttcttta cgtcggtgaa agatttttat acgaaataca
     5461 acttccagta cggcgggaac ctgaacgtca tcgtcgcgcg ccggacgcag gtaggtgttt
     5521 tttacaacat gtggtcgctt ggcggaaacc aggcgttcca gtctaacatg ggaaagaacg
     5581 atcgcgtgaa cggaacgcag tacgggcttg tcgtcaggaa tttccgtgca ggccgcggcg
     5641 gacttgcgcc gatcgggaaa ttctgggacg tgagcatgag ctatgcgtca aacacgtaca
     5701 aagcgggaag cgacaacaaa gatattcttg gaggaactcc cgaaaatgtt cccgtctcat
     5761 ccaaccagat cctggcgcat gttgcattcg gaacgcagat ggtgttctgg aatcatttcg
     5821 tcggaaatac gggagtacgt ttcggcggac cgatctacac gatcgacaag aacaacggcg
     5881 gctacaaaaa ctttttcgcg cagcgcatcg cgtacaaaga gttcttctcg gtgttcttcg
     5941 gcatcgggtt cctgatctga tattttttcc ggtaaaaaag aaagggagaa cgaacgtctc
     6001 cctttttgtt ttttagttcc atcacagggt cttctgctca tcctctcaag tcacattccc
     6061 cgctcatcct catccacagg ttttccgccc cgtccatgcg tattcgcagg tttttcccgc
     6121 ttcggcttga ccatcttgct gaacaggtcg cggtagaatt ccgttgtgcg gttgccttct
     6181 ttccctttgc gcaggagctg gtgcttcacg ttgatgactt cgcccgtttt gaggtcgagt
     6241 atggcggcgt cgaggaaaaa tttctcgcgc ggaatgaacg cccgcacggc aggcacgacg
     6301 aacgcgcagg acatggcgaa taatacggga tgctggatct tcctgtaata atcggcttcc
     6361 acgctggtga acatcacgta acgcgtgccg tatttttttg caatggaatc cgcgcgggac
     6421 tggtcattta ccgtccacgc ccagctgtga tttccatgct gcagcttttc atagaaccat
     6481 tcattcagga cagcgagatc ggaaagcgaa tcccctgcag tgctgtccat cgtcatcggg
     6541 ttgaggatga tcgcattcac accggcggct gacgcctgcg cttttatcac gcccgtcatg
     6601 cgcgtctggc cgtcctcgct catccggtaa tccatgtcgg gatcgtcgtt gcggttcttc
     6661 tgcttgtaat attcataatc gggcccggcg acgatgatct tcgtgatgcc gaaaccatag
     6721 ttcttttctt ctttctcatc ggctttccgt tcttttttcg tgcggaaatc gtacgtggaa
     6781 taatccggtg tgtagacgag gcttctccgg ttgtcggtgt aatatttgaa gcgcgaaaca
     6841 aattcggtat ccttcagcat ttcgacaaaa gcgaacttgg tgaaacgctc cttgccggaa
     6901 ttcagcttga atttatcgag gcgcgagaac ttggaatcgc cggtctcacc ggtttcctgc
     6961 acccgtgaaa gtgaatcttt ctgcagctgt gccttcgctt catcgggcgt cacggttgag
     7021 aaatagctga ggtgaagatt ctggttgttc accagcatgg aaaaaagact gtcgcaaagc
     7081 gtgctcagca atgaatcgga cggatttttc ttgtgcagcg tccagttata cgaaagcgcg
     7141 agaacggtca tctcgtccgg ctcgatctcg tggaagagat ggaagatctg ttcctgctga
     7201 cccgggttgt ctttgaaatc gggaagatgg taataacccg aacggttcag cgcatcgtat
     7261 tttccgccgt acgttccgaa aaatccccag aaatcataga tcatgaaacc tgttttcttt
     7321 cccgactggt aataagcggc gatctcgtac agcgattcgc cgacgatctt ctcgaggtac
     7381 gaatcgccgg gatgacgctg cagcatcatg tacgagcagt aaaccgccgc cgggtaattc
     7441 ctgtcctgca ggtaaagacg gcagatctcg tagcgtgcat tctcgcggct ggcaaggaaa
     7501 ttatctttcg aaaccataaa aagacttccg tctttgttct gcaccgtatc gagcgtggtc
     7561 atcagcgcca tcctgcgctt gcgcacgttc gggtgcgtgc tcccgagatc gtcgtagttg
     7621 tcgtcggtgt agatcgggtc gacttccttc atgaaatatc ccggggggat cttcatgtaa
     7681 tctttgttga agaacatcgt gtcgaacggc acgtcgtcga acggaagatc ggcgtactcg
     7741 agcacgtcga atgagcgcag cgcttctttt ttggcgtagc cggatttcat gaaaagctcg
     7801 aacccgaggc ggtccgcttc cagctcctgc tcctggttgt aggaatagta cgacgactgc
     7861 agcaggtaca tcggcgtttt tcctttctgg ttcttgagct cttcgcggtt gaggtaacct
     7921 ttcagcatgt gctgctgccg gaagtgcgtg atctcgtgac aaagaatgaa cgcgagctgc
     7981 gcctcgttct cgagctgcgc caggagcccg agcgtgatga gcacggtgcc ggtggaagaa
     8041 gtgtacgcat tcggatcggc atctttgtaa gcgtaaaaat gaagcttgtg cagcgtcgcc
     8101 gtatcgtcct tcagcacttc cgcacctacg ctgctgatgt aatcgctgat cgaatcatta
     8161 tacagcacgt aaccggaaaa catgcgctgc tgcatgaagt acgacgattc gtacatgaaa
     8221 tctttttcct ggtcggaaac aacgtcgccc tgtgcctggc gccgggcgat atccttgtcg
     8281 acctgctggt aaacgggaca ttggaaataa gccggcatcg ggcctttcgg ctcgagcggc
     8341 gcatgctgcg cgaaagcggg aaaagcgaaa aaaataaaga ataagccggg aagacgtctg
     8401 cgcatgtaaa ggagcgtttt aggtataact acaaattacg gaaaagattc acgcgatgcc
     8461 gtgaagcgat ccgcctgcta caaagaaacg atcaatccgc gtctctattg tgggatcttt
     8521 ttcaagcggc ggcgcatgat gtgtttttgt gcgcgcatcg atgataagcg ggccgcggca
     8581 accccaatgc ttgttttcat aaaaatcgtt aacaccataa atgtcgcggg ccgggttgct
     8641 ctttgtgaac gtcacccaca gccagctggc ggtatcgtgt gccgtgaact ggctgtcgtc
     8701 gcacaccacg atgatcatca atccttccgt ctgcggtttt ctttctgcga ggaatccgca
     8761 cagctgcgcg atctcctgtt tggctttttc agcggaagaa aagcgaggac cgctgatcgc
     8821 cgtgacgccg ggaaaaacga ggcgtgcgga atggaaatca ccgggaagct ccgtaagcag
     8881 gtgtgacgaa agcgtgcgct ttttttctcc gcatgcggca aataccactt tcgacccggt
     8941 gttcagtccg tccccgctgt aatcgagcgt gtcgatgctc gtgttggtat gaaaatgcac
     9001 gtcgcgtgaa agatcgatgc gttcgagtac gtgctggaaa aaaacaggta tgttttccgc
     9061 gtccggattt ttttcgcctg tttcttccgc gatgaaaaga tattttgcca gcgaaagctg
     9121 ccctgttccg agaatgtgac tggcgatggt gagcagctcc gacggacgtt tttccttcag
     9181 gtaaggcgtg taacgttctc ctcccgtggc cagcagcagc gggtgaacgc cggccgcatc
     9241 cacggcatga acctgcttca gtccagggat ctcgtcctgt attcctttcc ccgcgagctc
     9301 atgcaccagc cagccgaagc tcgtgtcttc ctgcggcgga cgtccgacga ccgtgaaagg
     9361 ccatatcgca tttttgcggt gccacacttt gtgaatgcgc atcacgggga acggatgggt
     9421 gaggctgtag tacccgaagt gatcgccgaa aggtccttcc ggtttgtttt caccgggatg
     9481 aacttctccc gtgatcacga aatccgcatc gcctgaaatg caaaaaccgt cgcgataacc
     9541 ataacggatc ttccggttgc cgagcacacc ggcgaacgtc atttcagaaa taccttccgg
     9601 caacggcatc accgcgctga aagtaagcga aggtggtccg ccgacaaaaa cgctcacctt
     9661 catcggcaga cctttcgcgt tccatttcga ctggtgcaca cctattcccc ggtgcagctg
     9721 gtaatgaaga ccggcttccc ggtcgcgtac gtattcattt ccgttcagct ggatgcggta
     9781 catgccgaga ttggaatgca tcacgccggg aaattcggga tcttccgtat aaacagcggg
     9841 cagcgtgacg aacgcacctc cgtcatccgg ccagtgcctg atctgcggca ggtcgtgcag
     9901 ccggatctca ttttcaagaa cggcaagcgc gctggttttt tttgggaacg atttaagtgc
     9961 cgcccatcct gttttccagt tacggaacgg gtgacgcaag gcttccgaag gatcggattt
    10021 cagccggatg agcgctttta cattttcgat cgcatcgcgg aaaataaaac gcgaacgttc
    10081 tttcgtgccg aaaagattcg atgcggcggg aaatgcacag ccttttacat tttcgaagag
    10141 aagggcagga ccgccggctt cgtgcacgcg gagatgaacg gcggccatct ccaggtcggg
    10201 atcgatcatt tctttgacgc gcacaagatg accgttgcgt tccaggtcac cgatgcaatc
    10261 acgcaggctg ctgtatccca tggctcaaag gtattatttc accacgtgga tttaattcag
    10321 ctccagcggt aaatgttctg tttttggtcg tgaaaaaacc catttgcctg cgttttgctt
    10381 cgctgatcct gttcctgttt tccacgctcg tactttctgc actggacgaa gtggatagtc
    10441 ttgagcgcct gctgaaacag gaggctcccg attccgtgcg gatcgaccgc ctgatgacgt
    10501 tgtcgaagat tttcatgtat tccggcggcg aagccagcct gaagaacgcg gatgctgcga
    10561 tgcggcttgc acacagctcc ggcgaccgca gccgggaaat gcaggcgtac gccacgaggg
    10621 gaaagacatt cctgctctga tcaattgctc atctcggcgc gcaggagcag aaagaaaagc
    10681 tggaaaagga attcatgtcg tggaaaggtt ccgtggagca ggtcgatgac gtgctggtga
    10741 ccgggatcat cgtttccgtt taaacgcggc ggcagacggc attgtacggg cagctcctgc
    10801 agttctgttc a
//
LOCUS       DASTSF010000409         3032 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1368391,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000409 DASTSF010000000
VERSION     DASTSF010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3032
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1368391"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..307)
                     /locus_tag="VFU15_11780"
     CDS             complement(<1..307)
                     /locus_tag="VFU15_11780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718511.1"
                     /translation="MKEQLDDNGLDKLFRDKTGSGEVAPPAKAWDNIRRQLDRKPRRK
                     AGWWWTAAFLLFAGAFSAWFFGRNDISFDDRKMTAGDSDYHKPVLSKKDVQPVSSTND
                     "
     gene            complement(304..870)
                     /locus_tag="VFU15_11785"
     CDS             complement(304..870)
                     /locus_tag="VFU15_11785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018476794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU4718512.1"
                     /translation="MSTEEDILDGCISGKRESQELLYKKFSGKMFAVCLRYCRDHEEA
                     QDVLQDGFVRVFGNISAFRRDGSLEGWIRRIMVNTALMHLRAKKREPDFSDIDNIAMR
                     YHPESYFDTAAQVNAQELMRMLEALPDGYRTVFNLFAIEGYSHKEIGEMLGITEGTSK
                     SQLSKARGFLQEMLQKVMRVSIKENTQR"
     gene            complement(934..1626)
                     /locus_tag="VFU15_11790"
     CDS             complement(934..1626)
                     /locus_tag="VFU15_11790"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU4718513.1"
                     /translation="MERTLLMLHGAIGASDQLERLSGEFSGAYGEVLFFDFPGHGCKA
                     AEDAEFSIPYFANALLAWMEEKNVVQPDVFGYSMGGYVALYIASHFPGKLGKIVTLGT
                     KFSWSGETALKEVKMLDPEKIKEKVPAFAAALEKRHSPGDWKTVLSKTTGMMLELGRD
                     PALKKQDFLRIATPVLLTVGGKDTMVGREETEEVHRLVAGSQLHVFRDTPHPVEMIDA
                     ALVSREALNFFR"
     gene            1681..2472
                     /locus_tag="VFU15_11795"
     CDS             1681..2472
                     /locus_tag="VFU15_11795"
                     /inference="COORDINATES: protein motif:HMM:NF042462.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbnP family protein"
                     /protein_id="HEU4718514.1"
                     /translation="MFIPGIKKSFPALLAASLIFFAAGCRKDTDQTEPVTPATGIILS
                     FSNVADTVPLTLNSATYNYVNANGDSFNVTTYKYYISNVSFTDANNNTWSEPESYHLV
                     NAADPNSLNVSISGIPAGNYTSIHFMIGVDSTRNVSGAQTGALDPANDMFWTWNTGYI
                     MAKFEGKSPSSTAAAHSLTFHIGGYGGANATQQWVTSSFGSQTANVTTSVIPVVHISS
                     NMLEWFRSPSVIDFSTTNSVASPGNNALTIAHNYADMFTVTGITN"
     gene            2472..>3032
                     /locus_tag="VFU15_11800"
     CDS             2472..>3032
                     /locus_tag="VFU15_11800"
                     /inference="COORDINATES: protein motif:HMM:NF015131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c peroxidase"
                     /protein_id="HEU4718515.1"
                     /translation="MTNRTRKKLIFACMGGAILLAGTFFNGCRVDPKVLPETTSEQVK
                     FYIPDGFPQPVYTFAGNEVTLAGFTLGRKLFYDPRLSADNTISCGFCHQAPASFANAN
                     HALSHGIGGQFGRRNAPVLVNLAWNQSFMWDGGINHIENQPPAPIQNPVEMGETMSNV
                     ILKLSAVPSYHDDFKAAFGDDSITTQR"
BASE COUNT          680 a          903 c          736 g          713 t
ORIGIN      
        1 aatcgttcgt gctgctcacc ggctgaacat cctttttgga cagcaccggc ttgtggtaat
       61 ccgaatcgcc tgccgtcatt ttccggtcgt cgaacgaaat atcgttccgc ccgaagaacc
      121 atgcagaaaa agccccggca aaaagaagga atgccgccgt ccaccaccag cctgctttgc
      181 ggcgtggctt acggtcgagc tgccggcgga tattgtccca cgctttcgca ggaggagcga
      241 cttccccgct tcccgtcttg tcgcggaaca atttgtccag cccgttatcg tccagctgtt
      301 ctttcatcgt tgtgtgtttt cctttatcga gacccgcatg actttctgca gcatttcctg
      361 caggaacccg cgcgctttcg agagttgact ttttgatgtg ccttccgtga tgccgagcat
      421 ctcgccgatc tctttgtgcg aatagccttc gatcgcaaaa agattgaaaa cggtccggta
      481 accgtccggc agggcttcga gcatacgcat cagctcctgt gcattcacct gcgcagccgt
      541 atcgaaataa ctttccgggt ggtaacgcat ggcaatgttg tctatgtccg aaaaatccgg
      601 ttcacgcttt ttggcccgca ggtgcatgag cgccgtattc accatgatcc ggcggatcca
      661 tccttcgagc gatccgtcgc ggcggaaagc gcttatgttc ccgaacaccc gcacgaaacc
      721 gtcctgcagc acatcctgcg cctcttcgtg gtcgcggcaa tatcgcaggc aaacggcgaa
      781 catttttccc gagaactttt tgtaaaggag ttcctgggac tcgcgcttac cggaaataca
      841 tccgtcaaga atgtcttctt cggtactcaa gtcgggtagc tttctattgt ggtaaagatg
      901 cgaaaagatg caggaaagtt gcctgtggcc cgcttaccgg aaaaagttca gcgcctcgcg
      961 ggaaacaagg gccgcatcga tcatttccac ggggtgcggc gtatcccgga aaacatgcag
     1021 ctgcgaacct gcaacgaggc ggtgcacctc ttccgtttcc tcccggccga ccatcgtgtc
     1081 tttcccgccg acggtcagta aaaccggcgt ggcgatgcgc aggaaatcct gttttttcag
     1141 cgcagggtct cttccgagtt ccagcatcat gcccgtggtt ttgctcaata cggttttcca
     1201 gtcgcccggc gaatgacgtt tttcaagcgc agcggcgaat gccggcactt tctcctttat
     1261 tttttccggg tcgagcatct tcacttcttt cagcgccgtt tccccgctcc acgagaattt
     1321 cgttccgagt gtgactatct tccccagctt tcccgggaaa tgcgaagcga tgtacagcgc
     1381 cacgtagccg cccatgctgt acccgaaaac atccggctgc acaacattct tctcttccat
     1441 ccacgcaaga agcgcattcg caaaatacgg tatgctgaac tccgcatctt ccgcagcttt
     1501 acagccatga ccgggaaaat cgaaaaataa aacctcacca tacgcgccgg aaaattcacc
     1561 ggaaagtctt tccagctgat ccgacgcacc gatggcgccg tgcagcatca gcaaagtacg
     1621 ctccatctga aagtgatatt aatcatttct tttttttctt caaataacat aactttaaaa
     1681 atgtttattc ccggaataaa aaaatcattt cccgccctcc ttgccgcttc gctgattttc
     1741 tttgccgcgg gatgccggaa agatacggat cagacggaac ctgtgacacc ggccacaggc
     1801 atcatccttt cgttctcgaa tgtcgcggac acggttccgc tcacgctgaa cagcgcgacc
     1861 tacaattacg tgaatgcgaa cggtgattcg ttcaacgtca cgacctacaa atattacatc
     1921 agcaacgtct ctttcaccga cgcgaacaac aatacgtggt cggaaccgga aagctaccac
     1981 ctggtcaacg ccgccgatcc gaactcgctg aacgtttcca tcagcggcat tcctgccggc
     2041 aattacacat ccatccattt catgatcggc gtggacagca cgcggaacgt ttccggggcg
     2101 caaaccgggg cgctggatcc tgccaacgac atgttctgga cctggaacac cgggtatatc
     2161 atggccaagt tcgaagggaa atcgccttct tccacagctg cggcgcacag ccttacgttc
     2221 cacatcggcg ggtacggcgg tgcgaacgca acgcagcagt gggttacttc ttcatttggt
     2281 tcacagacag ccaacgtcac caccagcgtt attcctgtcg tgcatatcag cagcaatatg
     2341 ctggaatggt tccgttcgcc cagcgttatc gatttcagca ccaccaattc ggttgcctcg
     2401 cccgggaata atgcgctcac catcgcgcac aattacgccg atatgttcac cgtaaccgga
     2461 atcaccaact gatgaccaac cgcacccgga aaaaactgat tttcgcctgc atgggcggag
     2521 cgatcctgct cgccgggaca tttttcaatg gttgtcgtgt cgatccgaag gtgctgcccg
     2581 aaacgacatc cgaacaggtg aaattctaca ttcccgatgg ttttccgcaa cctgtttaca
     2641 cgttcgcggg caacgaggtt acgctcgccg gtttcacgct cggcagaaaa ctgttttacg
     2701 atccgcgcct ttccgccgac aataccattt cctgcggatt ctgtcaccag gcgcctgcat
     2761 cttttgccaa tgcgaaccat gcgctgagcc atggaatcgg cggacagttc ggaagacgga
     2821 atgcgcctgt gctcgtcaat cttgcgtgga atcaatcgtt catgtgggac ggcggcatca
     2881 atcacatcga aaaccagccg ccggcgccga tacagaatcc cgtggaaatg ggagagacga
     2941 tgagcaacgt gatcctcaaa ctgagcgccg ttccctccta tcacgatgat ttcaaagcgg
     3001 ctttcggcga cgactcgatt accacgcagc gc
//
LOCUS       DASTSF010000410         6997 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_901305,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000410 DASTSF010000000
VERSION     DASTSF010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 6997)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6997)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6997
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_901305"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..910)
                     /locus_tag="VFU15_11805"
     CDS             complement(<1..910)
                     /locus_tag="VFU15_11805"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HEU4718516.1"
                     /translation="MGKRIAVIDLGTNTFNLLVAEITGPHSFISVYNEKLPVKLGEGG
                     INRGFIAEKAFHRGVQAMENYAATVRDWKADEVMAFATSAVRSASNGKEFVQTVKEKT
                     GISITIISGEEEAELICTGVRHAVKLSEEPSLIIDIGGGSTEFIIASRDKVFWKQSFE
                     IGASRLLQKFEPSDPITEKETAIVSAYLVDVLAPLWAAVQQYNVHELVGASGSFESLA
                     EMVTLQFHKIPFDEKETEFEFDLDECAVVHRQIMTSTRAQRLKMEGLISMRVDLIVVS
                     ALLVELIIRRLGITRMRLSTYALKEGV"
     gene            complement(914..1483)
                     /locus_tag="VFU15_11810"
     CDS             complement(914..1483)
                     /locus_tag="VFU15_11810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718517.1"
                     /translation="MNGIKALFCVLAAVAISSCGSDTSSSSQQSPVAGTDAGNTKDTA
                     VIPDTVQVTEIPKFDSVVDVGFTFLFTRAYCGGAAPPKEVLDKLNTPQALSLSTLILE
                     NKKTGNKIEVTTDANGKTEMNIPAGRYILRFSDKINPALPTGFNPKCETWLKMKVTEM
                     NIVPSGHVDKTVSITFECDPCDASAKMRP"
     gene            complement(1497..2864)
                     /locus_tag="VFU15_11815"
     CDS             complement(1497..2864)
                     /locus_tag="VFU15_11815"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU4718518.1"
                     /translation="MGKIGIIIRREYFTRIRNRTFIVMCFLAPLFFAGLVIVPPLLAG
                     KPGAPRKIVVVDASGIPDSLSYAFIFKDTLNLRFDYSYVYKGLTNVESAFKDSDQVSI
                     LYIPENFMGGGDTTQNHSIGLSVILKSTREPGFNTLNLLQSVLSAEVQRDMMKRNGIS
                     QRVMDLTLKKVAVDNEVKGHIGISEIKAVTGLIFGMVIYVYILLFGVLVMKGVVEEKT
                     TRIVEVIVSSVKPFQLMLGKIIGVALVGLTQFTAWIVLSFAIIIPVINHINEYKTDIS
                     QHTQANMTTGVAIQSGPRLMNFDITEETQKTVETIMSIPWGNLIPAFLFYFIFGYLMY
                     AAIFAAIGSGADKNADTSQFSAPVTIPLIISIASSASVLNDPDGPIAKWLSMIPFTSP
                     VVMLMRIPFGGVMVWELLLSVAILIVSFLLMTWLAGKIYRVGILMYGKKITWKELGKW
                     LFYKP"
     gene            complement(2870..3844)
                     /locus_tag="VFU15_11820"
     CDS             complement(2870..3844)
                     /locus_tag="VFU15_11820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008638149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU4718519.1"
                     /translation="MGILLSAKNIVKKFAGHTALDDVSIDVPEGTIFGLLGPNGAGKT
                     TLIRIINQITGPDSGRVLFEERLLEDAHIAQIGYLPEERGLYPKMEVGEQAMYLARLR
                     GLSKREAKKRIDEWFDRFGMHSWWKKKVEELSKGMQQKVQFIVTVLHEPRLLILDEPF
                     SGFDPINAQLIKDEILRLKANGATILLSTHNMGSVEELCDEIALINLSKKILDGNVKE
                     IRRAYGSRTYDIQFRGNMMGFTNAMWTGAELIEKHEDKEGLHKARIKLNGNTSPNTIL
                     ENVLRTAEIHSFNEVLPTMNDIFIMKVKENSGESIGQTVLGTKSNYTE"
     gene            3979..4500
                     /locus_tag="VFU15_11825"
     CDS             3979..4500
                     /locus_tag="VFU15_11825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020532544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEU4718520.1"
                     /translation="MMYDTAKAIEVLERTPAVIAGMLDGISGEWISSGEGPGTWSPYD
                     VVGHLIHGETTDWVTRMEIILSDKEDKTFPRFDRFAQFEESKGKTMHMLLEEFKAVRA
                     KNISILRSKNLTEKDLAKTGTHPSFGTVTLRQLLATWVAHDLNHIAQIARVMAKQYRE
                     ETGPWVEYLRILK"
     gene            complement(4549..5694)
                     /gene="dnaJ"
                     /locus_tag="VFU15_11830"
     CDS             complement(4549..5694)
                     /gene="dnaJ"
                     /locus_tag="VFU15_11830"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013632779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEU4718521.1"
                     /translation="MSKRDYYEILGVGKSASPEEIKKAYRQMAIKFHPDKNPGNKEAE
                     EKFKEAAEAYEVLSDADKRAKYDRFGHAGVGSSAAGGSGGFSSMEDIFSQFGDIFGGA
                     FSGGGFGGFGGGGRRVNRGTNLRIKVKLTLEEIANGVEKKIKVPKYVSCDKCHGSGAA
                     GGGGASTCSTCKGQGQVMQVVNTPFGRMQSAATCPTCNGTGSVITDKCKHCHGDGVVR
                     GEENISVKIPAGVHDGIQLSVSGKGNAGPRGGVPGDLIVVIEEIPHEHFMRDGNNLFY
                     EHHISIPDAAMGTNIEIPTLEGKARVKVEHGTQSGKILRLKGKGLPDLNGYHRGDLLV
                     NLQVWTPTHLSAEEKKLLEKLKDSQNFKPNPGKKEKNFFDRMREYFS"
     gene            complement(6039..6587)
                     /locus_tag="VFU15_11835"
     CDS             complement(6039..6587)
                     /locus_tag="VFU15_11835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009283501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEU4718522.1"
                     /translation="MTENKDKKPVNTEQPETDEQQHELPGEPENESAKKIAELEEQVS
                     TLNDKYLRLYSEFDNFRKRTAKERVEILSMAGADMIKSLLPVIDDFERALRNNEKATD
                     VAAVNEGVNLIAQKFRSILASKGLEPLESLGKPFDVDMHEAITNIPAPSEELKGKVVD
                     EVEKGYMLNGKVIRFAKVVVGS"
     gene            complement(6722..>6997)
                     /locus_tag="VFU15_11840"
     CDS             complement(6722..>6997)
                     /locus_tag="VFU15_11840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018620270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718523.1"
                     /translation="IWPYHVSDLQWWQNAAAMQYQVFSIPSNFLIDGNGVIVAKNLRG
                     EQLEKALRTLEVNKKHSKKRKPKKGKSGDSPFGFIDTRRRNNVLFTA"
BASE COUNT         1536 a         1831 c         1846 g         1784 t
ORIGIN      
        1 tcacgccttc cttcagcgca taagtcgaca ggcgcatgcg cgtgatgccg agacggcgga
       61 taataagctc gacgagcaat gcggaaacga caataagatc gacacgcatg ctgatcaggc
      121 cttccatttt caaccgctgc gcgcgcgtgg aggtcatgat ctgccggtgg acgaccgcgc
      181 attcatcaag atcgaactcg aactccgttt ctttttcgtc gaacggaatt ttgtggaact
      241 gcagggtgac catttccgcc agcgattcaa acgaaccgga agcgccgacg agctcgtgca
      301 cgttgtattg ctgcacggcc gcccagagcg gcgcaaggac atccacgagg taagcggaaa
      361 cgatcgccgt ttctttttcg gtaatgggat cggacggctc gaatttctgc agaagacgtg
      421 aagcgccgat ctcgaagctt tgcttccaga agaccttatc gcgggaggcg atgatgaact
      481 ccgtgctgcc gccgccgata tcgatgataa gtgaaggttc ttccgaaagc ttcacggcat
      541 gacgaacgcc ggtgcagatg agttccgctt cctcttcgcc gctgatgatg gtgatgctga
      601 tcccggtctt ttctttcacc gtctgcacaa attcttttcc gttcgacgcg ctgcgtacgg
      661 ctgacgtcgc gaaagccatt acttcatctg ctttccagtc gcgcacggtg gccgcataat
      721 tttccatcgc ctgcacgccg cggtggaacg ctttttccgc aatgaagccg cggtttatgc
      781 cgccttcgcc gagcttcacg ggaagctttt cattgtaaac agaaatgaag ctgtgtggtc
      841 ccgtaatttc agctacgaga agattgaacg tgttcgttcc gagatcgatt acggcaatac
      901 gtttacccat agatcaggga cgcattttgg cggacgcatc acagggatcg cattcgaaag
      961 tgatcgacac ggttttgtcg acatgaccgg aaggcacgat attcatttcc gtcactttca
     1021 ttttcagcca tgtttcgcac ttggggttga agccggtcgg aagggcagga tttattttat
     1081 cgctgaaacg aaggatgtag cgtccggcgg gaatattcat ttctgttttc ccgttcgcat
     1141 cggtggtcac ttcgatctta ttgcctgtct ttttattttc caggataagc gtcgacagtg
     1201 aaagcgcctg cggcgtgttc agcttgtcga gaacttcttt cgggggagca gcgccaccgc
     1261 aataggcgcg tgtgaagagg aaggtaaacc cgacatcgac aacagaatcg aacttcggaa
     1321 tttccgttac ctgcacggtg tccggaataa cagcggtgtc tttcgtattc cccgcatccg
     1381 tgccggcaac ggggctttgc tgtgaagaag aagatgtgtc gcttccgcag gaagaaatgg
     1441 cgacagcagc gagcacacag aaaagcgctt tgattccgtt catcgttccg tatgtttcag
     1501 ggtttgtaga aaagccattt gccgagctct ttccacgtga tctttttccc gtacatgaga
     1561 atgcccacgc ggtagatttt cccggcgagc catgtcatga gcaggaacga gacaatgaga
     1621 atggcgacgg aaagcagcag ctcccacacc atcacgcctc caaaaggaat acgcataagc
     1681 atgacaacgg gggatgtaaa aggaatcatg gaaagccatt tcgcaatggg accgtcggga
     1741 tcattcagca cggaagcgga tgaagcgatg gagatgatga gcgggatcgt gacgggggcg
     1801 gagaactggc ttgtgtctgc gttcttgtcc gcgccggaac cgatggccgc aaaaatggcg
     1861 gcgtacatga ggtaaccgaa aataaaatag aagaggaaag cgggaataag atttccccag
     1921 ggtatcgaca tgatcgtttc gaccgtcttc tgcgtttctt cggtgatgtc gaaattcatc
     1981 agcctcggtc cggactgtat agccacaccc gtcgtcatgt ttgcctgcgt gtgctgcgag
     2041 atatccgttt tgtattcgtt gatgtgattg atgacgggaa tgatgatcgc gaacgaaagc
     2101 acgatccacg ccgtgaactg cgtgaggccc accagcgcca cgccgatgat ctttccgagc
     2161 atgagctgga acggtttcac ggaggaaacg atcacttcca cgatgcgcgt tgttttttct
     2221 tccaccacgc ctttcatcac cagtacgccg aaaagtaaaa tgtacacgta aatgaccatt
     2281 ccgaaaataa gccccgtgac cgctttgatc tccgaaatgc cgatgtgtcc tttcacttca
     2341 ttatcgacag cgactttttt cagcgtaagg tccatgacgc gctgtgaaat gccattgcgt
     2401 ttcatcatgt cgcgctgcac ttccgcggaa agaacgctct gcagaagatt gagcgtattg
     2461 aagcccggtt cgcgcgtgga tttgagaatg acggaaagcc cgatgctgtg gttctgcgtt
     2521 gtatcgccgc cgcccatgaa attttccggg atgtaaagga tggaaacctg gtcggaatcc
     2581 ttgaacgccg attccacgtt ggtcagtccc ttgtaaacgt agctgtaatc gaagcgcagg
     2641 ttcagcgtgt ctttgaaaat aaatgcatag ctcagcgaat caggaatgcc ggatgcgtcg
     2701 acgacgacta ttttccgcgg cgcgccgggt ttcccggcca gcaggggtgg cacgatgaca
     2761 agcccggcga aaaacagcgg cgcgagaaag cacatgacga tgaacgtcct gttccggatg
     2821 cgcgtaaaat attcgcgccg tatgatgatg ccgatctttc ccatggcggc tattcggtgt
     2881 aattgctttt cgttcccaaa acagtttgcc cgatgctttc accggaattc tccttcacct
     2941 tcatgatgaa aatatcgttc atcgtcggga gaacttcatt gaaagaatgg atctcggccg
     3001 tacgcagcac attttccagg atcgtgttcg gcgaggtgtt tccattgagc tttatccgtg
     3061 ccttgtgcag accttccttg tcttcgtgct tttcgatcag ctccgcgccg gtccacatgg
     3121 cgttggtgaa tcccatcatg ttgccgcgga actggatgtc atacgtgcgt gagccgtacg
     3181 cgcggcgtat ttccttcaca ttcccgtcca ggatcttttt cgagagattg atcagcgcga
     3241 tctcgtcgca cagctcttcc acggaaccca tgttgtgcgt ggaaagaagg atggtggcgc
     3301 cgttcgcttt caggcggagt atctcgtctt ttatcagctg cgcattgatc gggtcaaacc
     3361 cgctgaaggg ttcgtcgaga atgagcaggc ggggttcgtg aagaacggta acgatgaact
     3421 gcaccttctg ctgcattcct ttcgataatt cctccacttt ctttttccac caggaatgca
     3481 tcccgaaacg atcgaaccat tcgtcgatgc gttttttcgc ttcgcgtttg gaaagtccgc
     3541 gcaggcgtgc gaggtacatg gcctgttcgc ccacttccat tttcgggtaa agtccgcgct
     3601 cctcgggaag ataaccgatc tgtgcgatgt gcgcatcttc cagcaggcgc tcttcgaaaa
     3661 gcacgcgtcc gctgtcggga cccgtgatct ggttgatgat gcggatgagc gttgtttttc
     3721 ctgcgccgtt gggaccgagc agcccgaaaa tggttccttc cggcacgtcg atgctgacgt
     3781 catcgagggc agtatgacct gcgaattttt tgacgatgtt ctttgccgaa agaagaattc
     3841 ccatgagtgg cgaagttaaa tgttaaccgg caaatgaacg ccggcaaaag aagaaaataa
     3901 tcggcgcgga agggggcatg tgatgttaaa attccgcaac ttgatcgttc aatcattcaa
     3961 cgttgttaat ttcattttat gatgtacgat actgcgaaag ccattgaagt gctcgaacgc
     4021 acgcccgccg ttattgccgg gatgctcgac ggaatttccg gggaatggat ttcttccggc
     4081 gaaggacccg gaacatggag tccctacgac gtggtggggc atctcatcca cggggaaacc
     4141 accgactggg tgacgcggat ggagatcatc ctttcagata aagaagacaa gacattcccg
     4201 cgtttcgacc gttttgcgca attcgaagaa agcaaaggaa aaaccatgca catgctgctg
     4261 gaggaattca aagccgtccg tgcgaaaaac atttccatcc tccgttcgaa aaatctgacg
     4321 gaaaaagacc tcgcgaaaac gggtacgcat ccttctttcg gcaccgtgac gctgcgccag
     4381 ctgctcgcaa catgggtggc gcacgatctc aatcacatcg cgcagatcgc gcgcgtgatg
     4441 gcgaaacagt acagggaaga aacgggtccg tgggtggaat acctgcggat actgaagtaa
     4501 aactcccggc ttattccgtg aagtcccgga agcaggaacc gcggtgtttc acgagaaata
     4561 ttcccgcatg cggtcaaaga aattcttttc tttcttgccg ggattcggtt tgaagttctg
     4621 cgaatccttc agcttttcga gcagcttttt ttcttccgcg gaaagatgcg tgggcgtcca
     4681 cacctgcagg ttgacaagca ggtcgccgcg gtgatagccg ttgagatcgg gaagaccttt
     4741 tcccttgagg cggagaattt tcccggactg cgttccgtgc tccactttca cgcgtgcttt
     4801 tccttcgagc gtgggaattt caatgttcgt acccatggcc gcgtcgggaa tgctgatgtg
     4861 gtgctcgtag aataaattgt ttccgtcgcg catgaaatgc tcgtgcggaa tttcctcgat
     4921 caccacgata agatcgccgg gaacgccgcc tcgcggacct gcatttcctt tcccgcttac
     4981 gctgagctgg atgccgtcgt gcacaccggc gggaattttc actgaaatat tttcctcacc
     5041 gcgcacgacg ccgtcgccat gacaatgctt gcatttgtcg gtaatgacgc tgcccgtgcc
     5101 gttgcacgtg gggcacgttg cggccgactg catgcggccg aacggcgtat tcaccacctg
     5161 catcacctgg ccctggcctt tgcacgtgga gcaggtgctc gctccgccac cgccggctgc
     5221 gccggaaccg tggcatttgt cgcaggaaac gtatttggga actttgatct ttttctcgac
     5281 accgttggcg atctcttcca gcgtcagctt cactttgatg cgcaggttgg tgccgcggtt
     5341 gacacgtcgt ccgccgccgc cgaacccgcc aaaaccgccg ccactgaacg caccgccgaa
     5401 aatatcgccg aactgggaga aaatatcttc catgctgctg aagcccccgc tgccgccggc
     5461 cgcgctgctt cccacgccgg catgaccgaa gcgatcatac ttcgcgcgtt tgtcggcatc
     5521 actcagcact tcgtaggctt cggctgcttc cttgaatttt tcttccgctt ctttgttgcc
     5581 cggattttta tcaggatgga atttgatcgc catctgccgg tacgctttct tgatctcctc
     5641 gggagaagcg cttttgccga cgcctaatat ttcgtaataa tctcgcttgg acatgctttc
     5701 tctgaaatgt tcaacgttta atgttcaacg ttttgttcgt tatgtacggt ccctgaacct
     5761 ggcgccttta aggccgggcc gggaaaggta gctcattaag tctgacggtt tttttgaaat
     5821 tacttccgct ttagcgaaat gagattcaac ttcttcctgc ctggtgtatt cccggacctt
     5881 taatctgaag tccccggaaa aattcccttc ccgcgttttt ctcaaaacct ccctgtcaag
     5941 cccgggagcc aaccgccggc tttcaatttc cgcgaagcgt ttaatggaag tcactttaaa
     6001 tccttgttgg ttgtttgtca aacattgacc ccggaacttt agcttccgac cacaactttc
     6061 gcaaaacgga tcactttccc gttgagcatg tatccttttt ccacttcatc cacgactttt
     6121 cctttcagct cttccgaagg agcgggaata ttcgtaatgg cttcatgcat atcgacatcg
     6181 aagggtttcc cgagcgattc caagggttct agccctttcg aagcgaggat gctgcggaat
     6241 ttctgcgcga taagattcac gccctcgttc acagctgcga catccgtcgc tttttcgttg
     6301 ttgcgcagcg cacgttcgaa atcgtcgatg acaggaagga ggcttttgat catgtctgca
     6361 ccggccatcg aaagaatttc cacgcgttcc tttgccgtgc gtttccggaa attgtcgaac
     6421 tccgaataca ggcggaggta tttatcattc agcgtgctca cctgttcttc cagctccgca
     6481 atttttttcg cggattcatt ctccggttcc ccgggcagtt cgtgttgctg ttcgtcggtt
     6541 tccggctgct ccgtattgac gggcttcttg tctttatttt cagtcataaa aatccggttg
     6601 aataaattca tggaaaggca ttctgcctgg ttcaaaatcc aggccagcaa agataatgcg
     6661 ccagaatggc agcgaaacgc ttttcgcagg gaaatacgtc agaaaatacc gtaaaaaagg
     6721 ctcaggcggt aaagaggacg ttattgcgcc ggcgtgtatc gataaagccg aaaggagaat
     6781 cgccggattt gccttttttc ggctttcttt ttttggaatg ttttttattc acttccagcg
     6841 tgcggagcgc tttttcaagc tgttccccgc gaaggttctt ggcaacgata acgccgttcc
     6901 cgtcgatcag gaaattgctg ggaatggaaa atacctggta ctgcatggct gcggcattct
     6961 gccaccactg gagatcgctg acgtggtaag gccagat
//
LOCUS       DASTSF010000411         3020 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_751170,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000411 DASTSF010000000
VERSION     DASTSF010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3020
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_751170"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="VFU15_11845"
     CDS             <1..252
                     /locus_tag="VFU15_11845"
                     /inference="COORDINATES: protein motif:HMM:NF016811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HEU4718524.1"
                     /translation="QIIAGVLLMFLFSFRMITLPRIGGDNLSCLVCGMKVDKPETYEY
                     DKKTYYFDTYNCKETFRMNPEKFLHNVCTPAPDTTKPNH"
     gene            326..478
                     /locus_tag="VFU15_11850"
     CDS             326..478
                     /locus_tag="VFU15_11850"
                     /inference="COORDINATES: protein motif:HMM:NF021378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HEU4718525.1"
                     /translation="MAFIVALFLAFSGNHNRVFSHETPLQILKRRFASGEISKEQFEE
                     MKKMLS"
     gene            complement(684..1358)
                     /locus_tag="VFU15_11855"
     CDS             complement(684..1358)
                     /locus_tag="VFU15_11855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718526.1"
                     /translation="MNSIFFRRSFTILLFLFVSLAAHAEKWIPFVIDSYVSAQAPWFI
                     SVRPDSAGTESWSCRDGQNLMVIMLVKSGAESPDDMDGLSNEYKRMISDYVSRLHGQL
                     TDSSTLDYNGVIAKKIHVQFKPPGNDTGYVADCIFIIGNNRVYTLQYLQPAALASSNH
                     DADYFLNSIKTDKYFTHDHQFKREDRSSALERGFVVGQVIGYLLILAALLYFLRKLLQ
                     RQNRLR"
     gene            complement(1660..>3020)
                     /locus_tag="VFU15_11860"
     CDS             complement(1660..>3020)
                     /locus_tag="VFU15_11860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HEU4718527.1"
                     /translation="PQGIVHGTTITVMNAARKISKSEEDRRGKLFVSAGLGGMSGAQP
                     KAGKISGLVSVIAEINPKATETRHSQKWVDEVHTDLGELITRIKKAQSAKEAVSLAYQ
                     GNVVDLWERLVEENMRVDIGTDQTSLHNPWAGGYYPAGISFEDSKKMMAEQPEKFKDE
                     VRASLKRHAAAVNKLAANGMYFFDYGNAFLLEASRAGADIMNSDGTFRYPSYVQDIVG
                     PMCFDYGFGPFRWVCTSADPKDLAYTDQLALGVLEDMHKNSPPEIRQQLSDNITWIKD
                     AMKNNLVVGSQARILYADSEARIRIASAMNAAIRDKKISAPVVLGRDHHDVSGTDSPY
                     RETSNIYDGSQFTADMAVQNFVGDGFRGATWISLHNGGGVGWGEVINGGFGMVLDGSD
                     DSERRLKSMLFWDVNNGIARRSWARNKEAMFAIEREMKRTPGLVVTMPNVVDDKLFEG
                     LV"
BASE COUNT          677 a          784 c          814 g          745 t
ORIGIN      
        1 cagatcattg caggtgtcct gctgatgttc cttttttcat tccggatgat taccctgccg
       61 agaataggcg gggataatct tagctgcctc gtgtgcggga tgaaagtgga taaaccggaa
      121 acatacgaat acgataagaa gacatactat ttcgatactt ataactgcaa ggaaacattc
      181 cgtatgaacc cggaaaagtt tttgcataat gtatgtacgc ccgcgccgga tacgactaaa
      241 ccaaatcatt aataaggagg accaggtcat gggatattat ggatatggaa tgcacatgtt
      301 aggatggttt gccattattc tgagcgtggc attcattgtt gctctttttc ttgcattcag
      361 cgggaaccac aaccgggtgt tttcgcacga aacgcccctc cagatcctca aacgccgttt
      421 cgcttcggga gaaatttcga aggagcagtt cgaggagatg aaaaaaatgc tgagctgaaa
      481 aagcgggttt gaaaaactgt gcggactgtt gttgtgcgta agtgtgagtt tggtggtgag
      541 gcaaattcag cgaaaacaga aacgggttgt ggacatggat gcttttgcga attgattacg
      601 ccgtcaattc ctgtgatcgc cgcaagcgtt ttgtgctgaa aaaacaattc cgcgtgaatg
      661 gagcaggaga tgtgcccgta tcgctaccgc agcctgttct gccgttgaag caacttcctg
      721 agaaaataga gcagggcagc aagaataaga agatacccga tgacctgccc gacgacgaac
      781 ccccgttcga gggcagagga acgatcttct cttttgaact ggtggtcgtg cgtgaagtat
      841 ttgtctgttt tgatggaatt gaggaaatag tccgcatcgt gattggagga agccagggca
      901 gcgggttgca gatattgcag cgtgtatacc cggttgttgc caattatgaa aatgcagtcg
      961 gccacgtagc cggtgtcgtt gccgggaggt ttgaactgca cgtgtatttt cttcgcaata
     1021 actccgttat aatccagtgt gctgctgtcg gtgagctgcc cgtgaagacg actgacatag
     1081 tcgctgatca ttcgcttgta ctcgttgctc agcccgtcca tatcgtcggg tgattctgcg
     1141 ccggatttga cgagcataat taccatcagg ttttgcccgt cgcggcagct ccagctttcc
     1201 gttccggccg aatcaggacg aaccgaaata aaccagggcg cctgtgcact gacgtaagaa
     1261 tcgatgacga acgggatcca cttttctgca tgcgcagcca ggctgacgaa gaggaacaaa
     1321 aggatcgtga aggacctgcg gaaaaaaata gaattcatac ggtatatgga tcgtgcaagc
     1381 tacaaaaaga ccggcaggtg cagcaaatgg cgccaggtgt tcgttcacgc aaaaccgcgt
     1441 gtgaaatttt attctgatgt tgcaaggctg cggtcatcct tcggtacgtc gcacttcgcg
     1501 cgactactca gtctgacgtt gaaaagttgg ggtcatcctt cggtacgcca tgttgtacgt
     1561 gattttctgt tcctgtgcgt tacattgaat cttttacgct tgtcagactg agtatccgac
     1621 gcaggaggat gtaccgaagg ctgacaagcg ccccatccct cacaccagcc cctcgaacaa
     1681 tttatcatcc accacgttcg gcatcgtcac cacgagtccc ggcgtgcgtt tcatttcacg
     1741 ttcgatggcg aacatcgctt ccttgttgcg cgcccagctg cgccgcgcga tgccgttgtt
     1801 cacatcccag aacagcatcg acttcagcct tctttcggaa tcatcgctgc cgtcgagcac
     1861 catgccgaag ccgccgttga tcacttcgcc ccagcccacg ccgccgccgt tgtgcagcga
     1921 gatccacgtc gcgccgcgga acccgtcgcc gacgaaattc tgcacggcca tgtcggcggt
     1981 gaactgagag ccgtcgtaaa tattcgacgt ctcacgataa ggtgaatctg ttccggaaac
     2041 atcgtggtgg tcgcggccga gcacgacagg cgcggagatt tttttgtcgc ggatagctgc
     2101 gttcatggcg gaagcgatac gtatgcgcgc ttcggaatcg gcgtaaagta tcctcgcctg
     2161 cgagccgacg acaagattat ttttcatcgc gtcttttatc cacgtgatgt tgtcgctgag
     2221 ctgctggcgg atctccggcg gagaattttt gtgcatgtct tccagcacgc cgagcgccag
     2281 ctggtcggtg tatgcaagat ctttgggatc ggccgaagtg cacacccagc ggaacggacc
     2341 gaaaccataa tcgaagcaca tcggtccgac gatgtcctgc acgtaggaag gatacctgaa
     2401 cgtcccgtcg ctgttcatga tgtcggcgcc cgcgcgtgaa gcttcgagca aaaacgcgtt
     2461 gccgtaatcg aagaaataca ttccgtttgc ggcgagcttg ttcactgcag cggcatgacg
     2521 tttcagcgaa gcgcgcactt cgtccttgaa tttttccggc tgctccgcca tcattttctt
     2581 cgaatcctcg aaagaaattc ccgcgggata ataaccgccc gcccacggat tgtgcagcga
     2641 ggtctggtcg gtgccgatgt cgacgcgcat gttctcttcc accaggcgtt cccacagatc
     2701 gacgacattt ccctggtagg cgagtgaaac ggcctccttc gccgactgcg cttttttgat
     2761 acgcgtgatc agttcgccga gatccgtgtg cacttcatcc acccattttt gcgaatggcg
     2821 tgtttccgtc gctttcggat ttatttccgc gatgacggaa acgaggccgg aaatttttcc
     2881 tgctttcggc tgtgcgccgc tcatgccgcc gagcccggca gacacgaaaa gtttcccccg
     2941 gcggtcttct tcactcttcg aaattttccg cgctgcgttc atcacggtga tcgtcgttcc
     3001 gtgcacgatg ccctgcggac
//
LOCUS       DASTSF010000412        12240 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1010056,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000412 DASTSF010000000
VERSION     DASTSF010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 12240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12240
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1010056"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2395)
                     /locus_tag="VFU15_11865"
     CDS             complement(<1..2395)
                     /locus_tag="VFU15_11865"
                     /inference="COORDINATES: protein motif:HMM:NF026255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel family protein"
                     /protein_id="HEU4718528.1"
                     /translation="MSFLKFLFVPAFGLLSANAAAHSFSGADVHGKVMNAENQPMQYV
                     VVTLLKTDDSTLVKGAVTDADGAYSMNGIEAGNYFVTASFTGYEKKWIGPVSISADAD
                     VPVENIVLMQSAEMSAVTISSVQPLFVQKPGMLIMNVENSPVRISGTAYDVICKAPGV
                     TVDQDGNFSLQGRGGVQVYMDGKPTYLSGEQLRSYLQNIPAAGIIRVELMTTPPAKYD
                     AEGSAGIINLVTKKSAQQGFNGSVSGGYGYGRTWKSEAGVSLNYGKPKFSVYAKYDLS
                     TPRRKEAKFVTRNVPFGGDTTRYDQDVTLMMEPFSQHASVGVDFYPSVKTTWGVRADG
                     FLFAQETNIETRNTVTQIDSNDINVLHQYNHLKGNFNNGSAGIYFRQDFDTNGTSLSA
                     NADYVLYNNRSHENYDLYFSDAGGNPLSSQFERALKSTDIGIYVGQVDYTHPFAKKYR
                     IETGVKSSYVVTANDLAFGNQDPFSGEWTNDTARSNDFTYRELINAAYASCSADYSKW
                     QLQLGLRAEQTISDGYSPTTKEDHKHDYIQLFPTLFVTQKINGRHSLQYSFSRRINRP
                     AYDELNPFIFYVDQYTYHVGNPLLQPEIVNSLDITHNYADMLFTNVGVSRTTAGIAEV
                     AHQVDSTGILNQSALNMNTIDYIYGSMTYSQQFTKWWINECNVSLTYARFRSNLYGVN
                     VDKGNTVFNASLTETFLLPDKWKFEVSGWYQSKMLYTIFIIAPSGDVSVGLSKNFLKN
                     KLRFSVNASDLFYTQTQRVSVGYSDQNLDAWHKFDSRVVYIRLRYNFSEGSATKKSQF
                     "
     gene            complement(2662..3648)
                     /locus_tag="VFU15_11870"
     CDS             complement(2662..3648)
                     /locus_tag="VFU15_11870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronic acid decarboxylase family
                     protein"
                     /protein_id="HEU4718529.1"
                     /translation="MKDQKRVLITGAAGFLGSHLCDRFIKEGCSVVGMDNLITGDLKN
                     IEHLMKLENFEFYHHDVSKFVFVPGQLDYILHFASPASPIDYLKIPIQTLKVSSLGTH
                     NLLGLARVKKARILVASTSEVYGDPTVHPQTEDYWGNVNPIGPRGVYDEAKRFMEAMT
                     MAYHTYHGLETRIVRIFNTYGPRMRLNDGRVLPAFIGQALRGEDLTIFGDGSQTRSFC
                     YVDDLVEGIYRLLMSDYANPVNVGNPQEITIRDFAEEIIKLTGTKQKVVYRDLPKDDP
                     KQRQPDISLAKKLLNWEPKIDRAEGLKRTYAYFKGLSKEELYKSEHREFGKK"
     gene            complement(3651..4967)
                     /locus_tag="VFU15_11875"
     CDS             complement(3651..4967)
                     /locus_tag="VFU15_11875"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019987176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HEU4718530.1"
                     /translation="MNIAVVGTGYVGLVTGTCLAETGNHVTCVDIDAGKVEKMRNGII
                     PIYEPHLDALFERNIKQGRLKFTVNLDEAVKQAKIIFLALPTPPGEDGSADLSYVLGV
                     AEELGLLMTDYKIVVDKSTVPVGTAEKVRAAIAKNCKTEFDVVSNPEFLREGFAVDDF
                     MKPDRVVIGTESERAQKLMEELYKPYVRQGNPILFMDEKSAELTKYAANAFLATKITF
                     MNEIANLCEKVGADVDKVRIGIGSDDRIGKRFLFPGIGYGGSCFPKDVQALALSAKEA
                     GQEMKILSAVMEINESQKTVIVPKIKKYFSNKLEGKKIAVWGLAFKPDTDDIREAPAL
                     YIIRELLASGARVAAYDPEAMDNVSRIFGGKIEFGIDEYSVLQDADALVICTEWSLFR
                     TPEFDKMKSLMKNNIIFDGRNLYSLGQMKEHGFGYISIGRAAVKNG"
     gene            complement(4983..6125)
                     /locus_tag="VFU15_11880"
     CDS             complement(4983..6125)
                     /locus_tag="VFU15_11880"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007095159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEU4718531.1"
                     /translation="MEQVTRKIQMVDLLSQYEKIRTEVDEAVLNVIRSSAYINGPEVK
                     SFRSELENYLGVKHVIPCANGTDALQIAMMALGLQPGDEVITADFTYVATAEVIGLLG
                     LTPVLVDVVPDTFDIDVNAIEKAITPKTKAIVPVHLFGQCADMEKIMAVAKKHNLFVI
                     EDTAQAIGAEYTFTNGKKQKAGTIGDIGCTSFFPSKNLGGFGDGGAIFTNDDDLAQKM
                     QMIANHGQSVQYYHDVIGVNSRLDSIQAAILRIKLRHLDEYAAARNKAAAYYDKAFAG
                     NAKIKTPARMKNSTHVFHQYTLQLNGVDREALKKFLAEKGIPAMIYYPVPLHLQKAYT
                     DPRYKKGDFPVTEKLCASVISLPMHTELDEDTLKYITGSVLEFVNA"
     gene            6230..7003
                     /locus_tag="VFU15_11885"
     CDS             6230..7003
                     /locus_tag="VFU15_11885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718532.1"
                     /translation="MKLLRYLFIFAFLFILPAGKLHAQLDVKDSVVSCGGVAFSYALQ
                     DPAADMADRFGLSSAVGAAGWYKTKKNLLFDLRWSYIFGKDLRQGEVLDSILTSDGHL
                     IDREGKIADIREFERGFTLQCGIGKIFNNIHGLSPNPNCGIILSGDFGYMQHKIRIYD
                     NGARAPQLAGDYLKGYDRLTGGFAVTGFLGYWYMSKSRYVNCFFGVEATQAFTHSLRS
                     WDFETMAPDTKKRNDELFGFRVGWNILIYQRSARSYYLN"
     gene            7010..8275
                     /locus_tag="VFU15_11890"
     CDS             7010..8275
                     /locus_tag="VFU15_11890"
                     /inference="COORDINATES: protein motif:HMM:NF016311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HEU4718533.1"
                     /translation="MRFLYSAGIFFFGILIRFALLFNGKARKWVDGRKNILAEIEKKL
                     SANTSPVAWFHCASLGEFEQGRPLIEAFRKENPGYFILLTFFSPSGFDVRKNYTGADF
                     VCYLPLDTKKNARRFIASAKPSVAFFIKYEFWLNYLDELAQKNIPHFLVSAVFRNDQV
                     FFRPWGRIFFRALQDYTWIFTQNADSDALLKNNRLANSSVAGDTRFDRVIEIASAEKD
                     IPVAAAFSSNSNVIVAGSTWPQDEMLLLPCLAEDLRHGTWKLIIAPHELSEEHCAEIE
                     KKLSDNGIVPQKIVRFSMAGNDIAEKQVLLIDNIGMLSSLYRYGKIAYIGGGFGAGIH
                     NTLEAAVYGIPVLFGPNYEKFSEAKILLARGGGFSFSSAAEIKTRLDALMNDETARAA
                     AGKNAGIFVQENKGATEKILSSCRGKINR"
     gene            complement(8265..10181)
                     /locus_tag="VFU15_11895"
     CDS             complement(8265..10181)
                     /locus_tag="VFU15_11895"
                     /inference="COORDINATES: protein motif:HMM:NF025486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative porin"
                     /protein_id="HEU4718534.1"
                     /translation="MRKYFLFFLFVIARSSIGLTQNSIAKKDTTTFPVDSTLFFSFRA
                     CGFDYGNTAVRQKLLLKLDGFQDYRPVTQNLGNAGSPEKKFLLPGFSPDAFRRGTSYF
                     DDFGFRAGNRQFYVSDRPYTNVSLVLGMKQEVNVYVRHAHPLGKNANVAFGFERIRST
                     GYYKRQNTNNTSVNLNGWFRSPGRRYAFLFDFSWTKDAVAENGGIANDSSFFFAKQLD
                     RQLVAVNLSSASTTCREREFWMKQFWSFGTVTDTVKIPGDSSGTVKTRILPSAAIVHT
                     LSVSDDYFLYSDNALTDGFYNVILKDSSSTSDSTYLWKMKNGLWFQVFNTKSDGKRRV
                     VSGKIGARHELGEIRNDTIYQSFSNIYADGSLDFYFGNNAFSAASLSGWYVASGYNAS
                     DRLIHLLMESDTIGKTLNLALEATSALSHPDFLAEHYSGNNFRWSNSFHQSGHSEAKM
                     LVLYRGKKNDNHYSVSATFSEFTDAIYFGQDFRPAQMNGNITAVTVSAFAETGLKMVR
                     TRSELTWNRIQYGSPIRLPEFVARESLYGNFHIFHNGLQLQFGVDATWFSAFYADAYM
                     PALSQFYVQNDSLSGNYLYISPWLSFKIKPVRVFIRADHANAAFSGRNYMIIPHYPQN
                     DFSLKFGLSWVFND"
     gene            complement(10289..10400)
                     /gene="rrf"
                     /locus_tag="VFU15_11900"
     rRNA            complement(10289..10400)
                     /gene="rrf"
                     /locus_tag="VFU15_11900"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(10453..>12240)
                     /locus_tag="VFU15_11905"
     rRNA            complement(10453..>12240)
                     /locus_tag="VFU15_11905"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT         2775 a         3347 c         3024 g         3094 t
ORIGIN      
        1 tgaactgtga ttttttcgtc gcacttcctt cgctgaaatt ataccgcagg cggatgtaca
       61 ccacgcgtga atcgaacttg tgccatgcgt cgaggttctg gtccgaatag ccaacggaaa
      121 cgcgctgcgt ctgcgtgtag aaaaggtcgc ttgcattgac cgaaaaacgc agcttgttct
      181 tcaggaaatt tttcgaaagc cccacgctca cgtccccgct cggcgcgatg atgaagatcg
      241 tgtacagcat cttcgactgg taccagcccg aaacttcgaa tttccatttg tccggcagaa
      301 gaaacgtttc cgtcagcgac gcgttgaaaa ccgtgttgcc tttgtcgacg ttcacaccat
      361 acaggttgct ccggaaacgc gcgtaggtga gcgatacatt gcattcattg atccaccatt
      421 tcgtgaattg ctgcgaatac gtcatgctgc cgtagatata gtcgatggtg ttcatgttca
      481 gcgccgactg gttgagaatt cctgttgaat ccacctggtg tgccacttct gcaattcccg
      541 cggtcgtccg gctcacacct acgttggtaa aaagcatatc tgcataattg tgcgtgatgt
      601 cgagactgtt cacgatctcc ggctgcagca gcggattccc gacgtggtac gtgtattggt
      661 cgacgtaaaa aatgaacggg ttcaattcgt catacgcagg gcggttgatc ctccgcgaga
      721 acgaatactg cagcgaatgc ctgccgttga tcttctgcgt aacgaataac gtcgggaaga
      781 gctgaatgta atcgtgctta tgatcttcct tcgtggtcgg cgaatagccg tctgaaatgg
      841 tttgctcggc gcgcaggccg agctgcagct gccatttgct gtaatccgca gaacagctgg
      901 catacgctgc gttgatgagt tcccggtacg tgaaatcatt tgaacgcgct gtatcgttgg
      961 tccattcgcc gctgaacgga tcctggttgc cgaacgccag gtcattcgcc gtgacgacat
     1021 agctgctctt cacgcccgtt tcgatgcggt atttttttgc gaacgggtgc gtgtaatcca
     1081 cctgcccgac gtaaatgccg atgtccgttg atttcagcgc tcgttcgaac tgcgacgaca
     1141 gcgggtttcc gccggcatct gaaaaataca ggtcgtagtt ctcatgcgaa cggttattgt
     1201 agagcacata atctgcgttt gcgctcagcg aagtgccgtt cgtgtcgaaa tcctgccgga
     1261 aataaattcc tgcgctgccg ttattgaaat tccccttcag gtggttgtac tggtgcagca
     1321 cattgatgtc gttcgaatcg atctgcgtca cggtattccg tgtctcgatg ttcgtttcct
     1381 gcgcgaaaag gaatccgtcc gcacgcacgc cccacgttgt tttcaccgac ggataaaaat
     1441 caactccgac gcttgcatgc tgcgaaaacg gttccatcat cagcgtgaca tcctggtcgt
     1501 acctcgttgt atcgcccccg aacggaacgt tacgcgtaac gaatttcgct tcctttctcc
     1561 gcggcgtgga aagatcgtat ttcgcataaa cgctgaactt cggcttcccg taattcaggc
     1621 tgactcccgc ttccgatttc catgttcttc cgtagccgta tccaccggaa accgaaccgt
     1681 tgaaaccctg ctgcgcgctc tttttcgtta caagattgat aattcccgcg cttccttccg
     1741 catcgtactt cgcaggcggg gtcgtcatca gctccacgcg gatgatgccg gccgccggga
     1801 tattctgcag gtagctgcgc aactgttcac ctgacaaata cgtgggcttg ccgtccatgt
     1861 aaacctgtac gccgcctctt ccctgcaatg aaaaattccc gtcctggtcc accgtcacgc
     1921 ccggcgcttt gcagatcaca tcgtaagctg ttccgctgat gcgcaccggg ctgttctcca
     1981 cgttcatgat gagcatgccc ggcttctgca cgaacaaagg ctgaacggaa gagatcgtca
     2041 ctgcgctcat ttccgcagac tgcatgagca cgatattttc gacaggaacg tccgcatctg
     2101 cagaaatgct caccgggccg atccattttt tttcatagcc ggtgaagctc gccgtcacaa
     2161 aataatttcc ggcttcgatc ccgttcatgc tgtacgcgcc gtctgcgtct gtcaccgcac
     2221 ctttcaccag cgtggaatcg tccgttttca gcagcgtcac gacaacatat tgcatcggct
     2281 ggttttccgc gttcatcact ttcccgtgca catccgcccc gctgaaggaa tgcgcagccg
     2341 cattcgcgga aagaagaccg aatgcaggca cgaatagaaa tttcaggaag gacatagcgt
     2401 tcattttggt ccgtgtcttt tgacgaacaa aaatgaaatc ttgttacacg cgacttcttt
     2461 cttttacacg aacggcaacg cgcgacgtgc agagtttgct tttctgcgga cgacaggaac
     2521 cgggaaattc caaatgaaat gttaacgctt ttcgagcggt cactgcagtg gatgaacgga
     2581 aagtgatttc aaacaggcgt caaagtccca gttcccaaaa gagaaaccgc gaaccggtca
     2641 gcccggaccg gcagcctggt ctcacttctt cccgaattcc ctgtgctcgc ttttgtagag
     2701 ttcttctttg gaaagacctt tgaaatacgc atacgtacgc ttcaaccctt ccgcacgatc
     2761 gatcttcggc tcccagttca gcagtttttt cgccagcgaa atatccggct gccgctgttt
     2821 cggatcatct ttcggaagat cgcggtaaac cactttctgc tttgtacctg taagctttat
     2881 gatctcttcc gcgaaatccc tgatcgtgat ctcctgcgga ttcccgacgt tcaccgggtt
     2941 cgcataatcg ctcatgagca ggcggtaaat tccttcgacc aggtcgtcca cgtagcagaa
     3001 cgaacgcgtc tgcgatccgt cgccgaaaat ggtgaggtct tctccgcgaa gcgcctgccc
     3061 gatgaatgcc ggcagcacgc gcccgtcgtt cagccgcatc cgcggaccgt acgtattgaa
     3121 aattcgcacg atgcgcgtct cgagcccgtg ataagtgtga tacgccatcg tcatcgcttc
     3181 catgaaacgt ttcgcttcgt cataaacacc gcgcggaccg atgggattca cattgcccca
     3241 gtaatcttcc gtctgcggat gaaccgttgg atcgccgtac acttccgagg tggaagcgac
     3301 gagtatccgc gcttttttca cgcgcgcaag tccgagaaga ttatgcgtgc ccagcgatga
     3361 aaccttcagc gtctggatag ggattttcag gtaatcgatc ggcgacgccg gcgaggcgaa
     3421 atgcagaatg tagtcgagct gccccgggac gaaaacgaat tttgaaacgt catgatgata
     3481 gaactcgaaa ttttcgagct tcatcaggtg ctcaatattt ttcaggtcgc ccgtgatgag
     3541 gttatccatg cccaccacgg aacaaccttc tttgataaaa cggtcacaca gatgtgatcc
     3601 gagaaaaccg gccgcgccgg tgatgagaac gcgtttctgg tctttcataa tcagccgttc
     3661 tttactgccg cccttccgat gctgatgtag ccgaagccgt gctctttcat ctgcccgagc
     3721 gaataaaggt tccttccgtc gaagatgata ttgttcttca tcagcgattt catcttgtca
     3781 aactccggtg tgcggaacag gctccactcg gtgcagatca ccagtgcgtc ggcgtcctgc
     3841 agcacggaat attcatcgat gccgaactcg atcttcccgc cgaaaatacg gctgacgttg
     3901 tccatcgctt cgggatcgta agctgccacg cgcgcccccg atgcgagcag ctcgcggata
     3961 atgtacaatg ccggtgcctc gcgaatatcg tccgtgtcgg gcttgaacgc aagtccccac
     4021 acggcgatct tttttccttc cagcttgttg ctgaaatatt ttttgatctt cggaacgatc
     4081 accgtcttct ggctttcgtt gatctccatt accgcgctga gaattttcat ttcctgcccg
     4141 gcttcttttg ctgaaagcgc aagcgcctgc acgtctttcg gaaaacagct tccgccatac
     4201 ccgatgccgg gaaaaagaaa acgcttcccg atgcggtcgt ccgaaccgat gccgatgcgc
     4261 actttgtcca catccgctcc aactttttcg cagaggttcg cgatctcgtt catgaacgtg
     4321 atcttcgtgg cgaggaatgc gttcgccgca tatttcgtca gctccgctga tttttcatcc
     4381 atgaaaagga tcgggtttcc ctggcgcacg taaggcttgt acagctcttc catcagcttc
     4441 tgcgcccgct cgctttccgt tccgatcacg acgcggtccg gcttcatgaa atcatccacc
     4501 gcaaaacctt cgcgcaaaaa ctccgggttc gaaacaacat cgaattccgt tttgcagttc
     4561 ttcgcaatgg ccgctcttac tttttcggct gtgcccaccg gcaccgtgct tttgtcaacg
     4621 acgatcttgt aatccgtcat cagcaggccg agctcttcgg caacgccgag aacgtaggaa
     4681 agatccgccg agccgtcttc tcccgggggc gtgggaagcg cgaggaaaat gatcttcgcc
     4741 tgcttcaccg cttcgtcgag gttgacagtg aacttcaatc gtccctgctt gatgttgcgt
     4801 tcaaaaagcg catcgagatg cggctcgtag atcgggatga tgccgttgcg cattttttcc
     4861 actttgcccg catcgatgtc gacgcacgtc acgtggtttc ctgtttcagc gagacaggtt
     4921 ccggtcacaa gacctacata acctgttccg acgactgcaa tattcataga attgttttga
     4981 atttatgcat tcacaaattc gagtacgctg ccggtaatat acttcagcgt gtcctcgtcg
     5041 agctccgtat gcatgggaag tgaaatgacc gacgcgcaga gcttttccgt cacggggaaa
     5101 tctccttttt tgtagcgcgg atcggtgtac gctttctgga gatgaagcgg tacgggataa
     5161 tagatcatgg ccgggatgcc tttttccgcg aggaatttct tcagcgcttc tcggtcgacg
     5221 ccgttcagct gcaacgtgta ctggtgaaaa acatgcgtgc tgttcttcat ccgcgccggc
     5281 gtttttattt tggcgtttcc cgcgaatgct ttgtcataat aagcagcggc tttatttctt
     5341 gcagcagcat attcatcgag gtggcgcagc ttgatgcgca ggatagccgc ctgtatgctg
     5401 tcgagacgcg aattcacgcc gatcacatcg tggtaatact gcacgctttg cccgtggttg
     5461 gcgatcatct gcattttctg cgcgagatcg tcatcgttcg taaagatcgc tcccccgtcg
     5521 ccgaagccac cgagattttt cgaagggaag aacgaggtgc agccgatatc gccgatcgtt
     5581 ccggctttct gttttttacc gttcgtgaat gtatattccg cacctatcgc ctgcgccgta
     5641 tcctcgatca cgaaaaggtt gtgctttttc gctaccgcca tgatcttttc catgtcggcg
     5701 cactgcccga aaagatgaac gggaacgatc gcttttgttt tcggggtgat cgctttttca
     5761 atggcgttca cgtcgatgtc gaatgtatcc gggaccacgt cgacgagcac cggtgtaagc
     5821 ccgagaagcc cgatcacctc tgccgtagcg acgtaagtaa aatccgccgt gatcacttca
     5881 tcccccggct gcaggccgag cgccatcatc gcgatctgca gagcatccgt tccgtttgca
     5941 cagggaatga cgtgctttac gccgagataa ttttccagct ccgacctgaa cgattttact
     6001 tccggtccgt tgatgtaagc ggaagagcgg atcacattga gaaccgcttc gtctacttcg
     6061 gtcctgatct tttcgtattg cgaaagaaga tcgaccatct ggattttccg tgtcacctgc
     6121 tccattggga ataacctgtt ttttcgggtc gtgcgaagat agtcattttg acccatacca
     6181 cctgtgaaaa tcaagccctt cgccttctga aaatggctat attagcgcga tgaaattgtt
     6241 gcggtatctt ttcattttcg cctttctttt tattcttcct gccgggaagc ttcatgcgca
     6301 gctggacgtg aaagattccg tcgtttcctg cggcggcgtt gcgttttcgt acgcgctcca
     6361 ggatcctgcc gctgacatgg ccgaccgttt cggattaagc tctgccgttg gcgctgcagg
     6421 gtggtacaaa acgaaaaaga acctgctgtt cgacctccgg tggtcgtata ttttcggaaa
     6481 agatctgcgc cagggcgaag tgctcgacag cattctcacg tccgacggtc acctcatcga
     6541 ccgcgaagga aagatcgccg acatacgtga gttcgaacgc ggcttcacgc ttcagtgcgg
     6601 catcgggaag atcttcaaca acatccacgg actttccccg aatcccaatt gcggcatcat
     6661 tctctccggc gatttcggct acatgcagca caagatccgc atctacgaca atggagcaag
     6721 agcgccgcag ctcgccggcg attacctgaa agggtacgat cgcctcaccg gcggatttgc
     6781 cgtcaccgga tttctcggct actggtacat gagcaaaagt cgttacgtga attgtttttt
     6841 cggcgtggaa gcaacccagg cgttcacgca cagcctgcgt tcctgggatt ttgaaacgat
     6901 ggctcccgat acgaagaaac ggaacgacga gcttttcggg ttccgcgtcg gctggaacat
     6961 tctcatttac cagcgctcgg cgcgcagcta ttacctcaat tgatatcgga tgaggtttct
     7021 gtattccgca ggtatttttt tcttcggcat ccttattcgc tttgcgttac tctttaacgg
     7081 gaaagcccgg aaatgggtcg acggaagaaa aaacattctt gccgaaatcg aaaaaaaatt
     7141 atcggcaaat accagcccgg ttgcatggtt tcattgcgca tcgctcggag aattcgagca
     7201 gggacgcccg ctcatcgagg cattccgcaa agaaaatccg ggttatttca tactgctcac
     7261 ctttttttca cccagcggtt tcgatgtccg caaaaactat acgggtgctg atttcgtctg
     7321 ctacctgccg ctcgatacga aaaagaacgc ccgccggttt atcgcttcgg caaaaccttc
     7381 ggttgcattt tttatcaagt atgaattctg gctgaattac ctggatgaac tggcgcaaaa
     7441 aaacattccg cactttcttg tttcggcggt cttccgcaac gaccaggttt ttttcaggcc
     7501 gtggggacgt attttttttc gtgcgttgca ggattatacg tggattttca cgcagaacgc
     7561 tgattcggac gcattgctga aaaacaatcg cctggccaat tcatccgtcg ccggcgatac
     7621 acgcttcgac cgcgtgattg aaattgcctc cgctgaaaaa gatattcccg tggctgcagc
     7681 attcagcagc aattccaacg tgatcgtggc cggaagcacc tggccgcagg acgaaatgct
     7741 tttgcttccc tgccttgcgg aagatctgcg gcacgggaca tggaagctga tcattgcacc
     7801 gcatgagcta agtgaagaac attgtgctga gattgaaaaa aaactttcgg acaacggcat
     7861 agttcctcag aagatcgtcc gtttcagcat ggcaggaaac gacattgcgg aaaaacaggt
     7921 tcttctcatc gataatatcg gaatgctttc ttctctttac cggtatggga aaatagcata
     7981 catcggcggt ggttttggcg ccgggatcca caacacgctg gaagctgctg tgtacggaat
     8041 tcccgttctt ttcggaccga attatgaaaa gttcagcgag gcgaaaattc ttttagcacg
     8101 cgggggcgga ttttcttttt cttccgcagc agaaataaaa accaggctgg atgcattgat
     8161 gaatgatgaa accgcaagag ctgctgcagg aaaaaacgcc gggatatttg tgcaggaaaa
     8221 taaaggagca acagagaaga tcctttcttc ctgccgcggg aagatcaatc gttgaacacc
     8281 caggaaagac cgaacttcag ggaaaaatca ttctgcggat aatgcggaat gatcatgtaa
     8341 ttccttccgg aaaaagccgc attggcgtga tccgcgcgga taaacacgcg gacaggtttt
     8401 atcttgaagc tcagccacgg tgaaatatag agatagttcc cggaaagcga atcgttctgt
     8461 acatagaact gcgacagcgc aggcatgtag gcgtccgcat aaaaagcaga gaaccatgtt
     8521 gcatccaccc cgaactgcag ctgcaagccg ttatggaaaa tatgaaagtt cccgtaaaga
     8581 gattcgcgcg ccacgaattc cggaagccgt atcggcgatc cgtattgtat cctgttccag
     8641 gtcagctcgc tcctcgttcg taccattttc aatcccgttt ccgcaaaagc tgaaacagta
     8701 accgcggtta tatttccgtt catctgggcg ggccggaaat cctggccgaa ataaatcgcg
     8761 tccgtaaact ccgagaacgt tgcgctgaca gaatagtggt tgtcattttt ctttcccctg
     8821 taaagcacca gcattttcgc ttcggaatgt cccgactggt ggaatgaatt cgaccagcgg
     8881 aagttgtttc ccgaataatg ctcagcaaga aaatccgggt gcgacaatgc ggatgtggcc
     8941 tccagcgcca ggttcagcgt cttcccgatc gtatcgcttt ccatcagcag gtgaatgagg
     9001 cggtcagagg cattgtaacc tgaagcgacg taccatcccg aaagcgatgc cgcggaaaat
     9061 gcattgtttc caaaataaaa atccagcgat ccatccgcat aaatattcga aaaggactga
     9121 tagatcgtgt cgttcctgat ttcgccgagc tcgtggcgtg cgcctatttt cccggaaaca
     9181 actctgcgtt ttccatcgct tttcgtattg aatacctgga accagagacc gtttttcatt
     9241 ttccacaggt aagtcgaatc gctggtggat gaggaatctt taagaatcac gttgtaaaat
     9301 ccatcggtca acgcattatc ggaataaaga aaataatcat ctgacaccga aagggtatga
     9361 acgattgctg ctgatggcag aatgcgtgtt ttaactgtgc ccgaggaatc accgggaatt
     9421 tttacagtat ctgtgacagt gccgaaagac cagaattgct tcatccagaa ctcccgttcc
     9481 ctgcaggtgg tcgatgcgct gctcaggttg acggccacaa gttgccggtc caactgtttg
     9541 gcaaaaaaga aagaagaatc attggcaatt ccgccgttct cggccacggc atcctttgtc
     9601 catgaaaaat cgaacaggaa tgcgtaacgt cttccgggag accggaacca accattaagg
     9661 ttgacgcttg tattgtttgt gttttgtctt ttgtaatacc cggttgagcg gatccgctca
     9721 aatccgaagg cgacattcgc atttttccca agcggatgcg cgtgacgaac atagacattt
     9781 acctcctgct tcattccgag aacgagcgag acattggtat aaggccgatc ggaaacgtaa
     9841 aattgcctgt tgcctgcccg gaagccaaaa tcatcgaaat aggaagttcc ccgccggaac
     9901 gcatcgggag aaaaaccggg aagaaggaat ttcttttcag gcgagcctgc gtttccgagg
     9961 ttctgggtga caggtctgta atcctggaac ccatcgagct tcagaagaag tttctgccga
    10021 accgccgtat tgccataatc aaagccacag gcacggaagg agaaaaagag tgttgaatcg
    10081 acggggaatg ttgtggtgtc ttttttggcg atagaatttt gcgtcagacc gatggacgac
    10141 cttgcaatta caaaaagaaa aaacagaaaa tatttcctca attgaaatgg cacacgcgga
    10201 acatttcggt aaaaataact gaaagaaaaa aagggagggg ataaaaaaag tgaaaccctg
    10261 atttttacaa accagggttt cgatctaaaa ctggcagcga catactctcc caccttgtag
    10321 ggcagtacca tctgcgatga cgggcttaac ttctctgttc gaaatgggaa gaggtgaaca
    10381 ccgtctctat agccaccatg aatcttaaat atttttataa caaattggaa ggaaacgcac
    10441 cagaagcgta ttcaaagaaa gtcgatcgag caattagtac cgctcggctt tgacattgct
    10501 gcctttacac ctgcggccta tcgacgtagt agtcttctac ggctcttaag ggacatctca
    10561 tcttgaggcg agcttcgcac ttatatgctt tcagcgctta tctcatccag acatagctac
    10621 cctgcgctgc acctaggcgg cacaacaggt acactagcgg tctgtccgac tcggtcctct
    10681 cgtactagag tcaggacccc tcaaatgtcc aacgcccaca acagataggg accgaactgt
    10741 ctcacgacgt tctgaaccca gctcgcgtgc cactttaatc ggcgaacagc cgaacccttg
    10801 ggaccttctc cagccccagg atgtgacgag ccgacatcga ggtgccaaac cactccgtcg
    10861 atgtgaactc ttgggagtga taagcctgtt atccccggag taccttttat ccgttgagcg
    10921 atggcccttc cattcagaac caccggatca ctatgacctg ctttcgcacc tgctcgaccc
    10981 gtcggtctcg cagtcaagca cccttatgcc tttgcactcg acgcccggtt tccaatcggg
    11041 ctgagggtac cttcgtgcgc ctccgttact ctttgggagg cgaccgcccc agtcaaacta
    11101 cccaccagcc agtgtcctgc gcccggatca cgggcgacag ttagacacca gaatccatca
    11161 gggtggtatt tcaccgttgc ctccaccgaa cctagcggcc cggcttcact ggctcccacc
    11221 tatcctacac aaacgaatcc tagtgtcact ggcaagttgt agtaaaggtt cacggggtct
    11281 ttccgtcttg ctgcgggtaa actgcatcgg cacagctatt tcaatttcgc tgagtccctg
    11341 gtcgagacag cgcggcagtc gttacgcctt tcgtgcaggt cggaacttac ccgacaagga
    11401 atttcgctac cttaggaccg ttatagttac ggccgccgtt taccggggct tcggctcacc
    11461 gcttcgcttg cgctgacgga tccccttaac cttccggcac cgggcaggcg tcagacccta
    11521 tacatccgct tgtcgcgttt gcagagtcct gtgtttttgg taaacagtcg ctaccgccta
    11581 ttctctgcga cccagttccg ctacgggagc aagtcccttg acgtacgctg ggcccacctt
    11641 cttccgaagt tacggtggca atttgcctag ttccttgacc agagttctct caagcgcctt
    11701 aggattttct cctcgcccac ctgagtcggt ttacggtacg gaatccaaaa tggctccacg
    11761 cgcggctttt cctggaagca tgggattatc gacttccggc cttacggcct cgtcacctgc
    11821 ctcagcgtaa tgtctccacc ttttcatcgt atggagaccg cctacacagg caaaccggga
    11881 cgtccaacac ccggatcgac tacccttctc cgtccccgct cgcttcaacg ccatcaagga
    11941 tgcgcaggaa tattaacctg ctgtccatcg cctacgcctt tcggcctcga cttaggatcc
    12001 ggctgaccct gggcggattg accttcccca ggaaaccttg ggcttacggc ggacatggtt
    12061 ctcacatgtc ttatcgctac tcatttcggc atcagcactc ccagaacctc caccgcttct
    12121 tgcgatacgg cttcgacggc gctgggacgc tcccctaccc ctcatctttc gatgaagtcg
    12181 cagcttcggc tctgcacttg agccccgtta cattttcggc gcagcctgac ttgaccagtg
//
LOCUS       DASTSF010000413         5162 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1152090,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000413 DASTSF010000000
VERSION     DASTSF010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5162
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1152090"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..572
                     /locus_tag="VFU15_11910"
     CDS             <1..572
                     /locus_tag="VFU15_11910"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEU4718535.1"
                     /translation="SIISSIADTRGVIVYANKKFCEVTGYSKEELIGQDHSIINSGYH
                     PKEFFRNMWKTIASGGVWHDEIRNRARNGTYYWVDTVILPIRDENGKTTHYLSLRMLI
                     TEQKKLEEEKKEYTHSLEEMLFLTSHKVRQPLATCLGLLNLAETKTLSGEEMLDIFRL
                     IRVSAMQLDDFTRELTGFIFRIKERNKDA"
     gene            complement(584..2635)
                     /locus_tag="VFU15_11915"
     CDS             complement(584..2635)
                     /locus_tag="VFU15_11915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718536.1"
                     /translation="MKRILLCGVFFAVVSALNAQRDSLQYAPGYPAYRVSQYDQLCSF
                     PLPRFKPGNTLHRNFSWFDFMYMAYDYSQVTKGQTGSFVYPGMKQAAHAQAAIDMNVE
                     MYSHWNYYYNIPTPTYNYQDFSRPNTFSGKITAYADRHPGMAVCTYIFWGAVRPSLTG
                     DPSRTSYIKKFESIDPCAGDTTYPKIRIDGNAQRYCLQKLADALPHRDSLHKVDFVNE
                     NGEIFGPSWYPNGEGYNRDPRIACIQHDSATARVNRGHWQYTVFNAYKRRFTANDSFP
                     AFRNSEFSFYQVSAFLPRYYGEYSEMRRINQYGRGGFYATPDFYPGDANHSIFDRYAA
                     YHGLDCIAEGRKTEIALGDDYFSPFVCAGWFADSLNYRPAQWLAAMKALAMMGAEYYY
                     PAFFNTSNPSVKMPVDPRGYMYQVVMPIYAQAVTSRFEPVFFGTKDFTYANEKGLLSI
                     YRHQVGNGKNIYAIHASDFSGEKKYDAAHPATRKIVLDGERITVNFRPQGSTYIYDKS
                     DSANVIFYQLDAWHENAHPWYWSKNFDFEAELHDDSNAVLLHTDRPAGAARGDYTDFT
                     TYVYFTDRNCSVPLGYYFEPRNNDSSTYYVWVRARCATAGKKTSCSIFADGKKCGNVM
                     DIQPGEFRWYVMGTTKDPAKVEAATGQQHLLQLKAGDSNLQVDRIILSTTKVPAEIPA
                     K"
     gene            complement(2713..4179)
                     /locus_tag="VFU15_11920"
     CDS             complement(2713..4179)
                     /locus_tag="VFU15_11920"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008242130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEU4718537.1"
                     /translation="MEPQKGKAKAYESELNDWISNERAAIELIGHIGTLWFERSAELI
                     IFRNQMIDRSPSEIMNLHQYAKDIVKRPINVRDTANLACELTRLELAPSRIDIGRLCA
                     EWTEEKGNYSDMKAFVTDKLKDYIGKEKASIVPKDVVLFGFGRIGRLAARELISQAGK
                     GDQLRLRAIVTRGNSDEEIVKRADLLRMDSVHGPFPGTIIEDLENKALLINGHLVHMI
                     HAANPEDVDYTAYGINNALVIDNTGVFRDREGLGRHLKAKGVAKVLLTAPGKGDIPNV
                     VYGVNHETVKPDSDKVFSAASCTTNAIMPILYVVDKELGVEKGHIETVHSYTNDQNLL
                     DNYHKKYRRGRSAALNMVITETGAESALKKVLPQLAGKFTANSVRVPTPNVSLAILNI
                     TVKRETSKEEVNEIVRHYALKGVLVEQIQYAFSNELVSSDVIGNSCSSVFDSQATIVS
                     PDKKSIVLYVWYDNEYGYTRQVLRFSKYLAEVIRLRYY"
     gene            complement(4304..>5162)
                     /locus_tag="VFU15_11925"
     CDS             complement(4304..>5162)
                     /locus_tag="VFU15_11925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008625412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HEU4718538.1"
                     /translation="QLKITKSITNRESASLDKYLQEIGREELITAEEEVVLAKKIREG
                     DERALAKMVKANLRFVVSVSKQYQNQGLSLPDLINEGNLGLIKAARRFDETRGFKFIS
                     YAVWWIRQSILQALAEQSRIVRLPLNQVGSLNKINKAYSRLEQEFEREPTAEELSQVL
                     DLPEDKVADTMRVSGRHVSMDAPLLNGEDNSLLDVIVNHDSPRADRLLMNESLQKEIE
                     RSLATLTERERDVIKLFFGIGCQHGLTLEEIGAKFDLTRERVRQIKEKAIRRLRHSSR
                     SKLLKAYLG"
BASE COUNT         1174 a         1360 c         1370 g         1258 t
ORIGIN      
        1 aaagcatcat ttcctccatt gcggatacaa ggggcgtgat cgtttacgcg aacaaaaagt
       61 tctgcgaggt gacgggatat tcgaaggaag agctgatcgg gcaggatcat agcatcatca
      121 attccgggta tcacccgaaa gaatttttca ggaacatgtg gaagaccatc gcgtccggcg
      181 gcgtatggca tgacgagatc aggaaccggg ccaggaatgg aacctattat tgggtggaca
      241 cggtcatact tcccatccgc gatgaaaatg gaaaaacgac gcattacctt tcgctgcgca
      301 tgctgatcac cgagcagaaa aagctcgaag aggaaaaaaa ggaatacacc cattcgctgg
      361 aggaaatgct ttttctcact tcgcataaag tacgccagcc gctggccacc tgcctcggtc
      421 tactgaacct cgccgaaacg aaaacgctct ccggcgaaga gatgctcgat atattccggc
      481 tcatccgggt atccgccatg cagctcgatg attttacacg cgagctgacc ggtttcattt
      541 tccgtataaa ggaacggaac aaggatgcgt aacacccgcg ttcttatttc gcaggaattt
      601 ccgccggaac tttcgttgtg ctgagaatga tccggtccac ctgcaggttg ctgtcgccag
      661 ccttcagctg aagaagatgc tgctgcccgg tcgcggcttc caccttcgcc ggatcttttg
      721 ttgttcccat cacataccac cggaattccc cgggctgaat atccatcaca ttcccgcatt
      781 tctttccatc ggcaaaaatg gaacaggatg ttttcttccc ggccgttgcg caccgcgcac
      841 gaacccatac gtagtaggtc gatgaatcat tgttccgcgg ttcgaaataa taaccgagcg
      901 gtacagagca attcctgtcc gtgaaataaa cgtacgtggt aaaatcggtg taatcgcctc
      961 tcgctgcccc ggcgggacgg tccgtgtgca gcaaaacggc attggaatcg tcgtgcagct
     1021 ctgcctcaaa atcaaaattc ttcgaccagt accagggatg tgcattctcg tgccaggcat
     1081 cgagctggta gaagatgaca ttggccgaat ctgatttgtc ataaatgtac gtcgagccct
     1141 gcggccggaa attcacggtg atcctttccc cgtcgagcac gatcttccgc gttgcaggat
     1201 gcgcagcgtc gtattttttt tcgccggaaa agtcggatgc gtgtatggca taaatatttt
     1261 tcccgtttcc cacctggtga cgataaatgg acaacaatcc tttttcgttc gcgtatgtga
     1321 aatcctttgt tccgaaaaaa acaggttcaa aacgcgacgt cacggcctgc gcataaatag
     1381 gcatgaccac ctggtacata tagccccgcg gatcgacagg catttttacg gaaggattcg
     1441 acgtgttgaa gaatgcgggg taataatatt ccgctcccat cattgccagt gctttcatcg
     1501 cagccagcca ttgcgccggg cggtagttca gcgaatcggc gaaccagccg gcacaaacga
     1561 agggtgaaaa ataatcatcc ccgagagcga tctccgtttt tcttccttcc gcaatgcaat
     1621 cgagcccgtg ataggccgcg taacggtcaa aaatggaatg gttcgcgtcg ccgggataaa
     1681 aatcgggcgt ggcgtaaaat cccccgcgac cgtattgatt gatgcgacgc atttcggaat
     1741 attccccgta atagcgcgga agaaaagcgg acacctggta gaaggaaaat tcagaatttc
     1801 tgaaagccgg gaaggaatcg ttcgcagtga accttctttt gtacgcgttg aacaccgtat
     1861 actgccagtg accgcggttg acgcgggccg tagccgaatc atgctggatg caggcaatgc
     1921 gcggatcacg gttatagccc tccccgttcg ggtaccagct tggcccgaaa atctccccgt
     1981 tctcattcac gaaatccact ttgtgaagcg aatcgcggtg cggaagcgcg tcggccagct
     2041 tttgcaggca atagcgttgc gcatttccgt cgatgcggat cttcggatag gtggtgtcgc
     2101 ccgcacacgg atcgatgctt tcaaactttt tgatgtaaga ggtcctcgac ggatctccgg
     2161 tgagcgacgg ccttactgct ccccagaaaa tatacgtgca caccgccatg ccgggatgcc
     2221 tgtccgcgta ggcggtgatc ttccctgaaa atgtgttcgg tcgtgaaaag tcctggtaat
     2281 tataggtcgg cgtgggaatg ttgtaataat aattccagtg gctgtacatt tccacgttca
     2341 tgtcgatcgc ggcctgcgca tgcgccgcct gcttcatccc gggataaaca aaactcccgg
     2401 tttgcccttt cgtcacctgc gaataatcgt aggccatgta cataaaatcg aaccaggaaa
     2461 aattccggtg cagcgtattt cccggtttga aacggggaag cggaaacgag caaagctgat
     2521 cgtactgcga aacacggtac gccgggtaac cgggagcata ctgcagggaa tcccgctgcg
     2581 cgttgagcgc tgagacaact gcaaaaaaaa cgccgcacaa aagaatacgc ttcatggttc
     2641 ccgtgatcaa aacaaatgcc gcaaaaaaaa gcccccgtca cttgtgcggg ggcgaagctg
     2701 gctgaattct tatcaataat accggagacg gatcacttct gcaaggtatt tcgagaagcg
     2761 aagcacctgc cgggtgtaac cgtattcgtt atcgtaccac acatacagga caatgctttt
     2821 cttgtccggc gaaacgatcg tggcctggct gtcgaaaaca gaagagcagg aattcccgat
     2881 aacgtccgag ctgacgagct cattggaaaa tgcatactgg atctgctcca cgagcacgcc
     2941 cttcagtgca taatgccgca cgatctcgtt cacctcttct ttcgaggttt cgcgcttcac
     3001 cgtaatgttc aggatcgcca gcgaaacgtt cggggtaggc acacgcacgg aattcgccgt
     3061 gaattttccg gccagctgcg ggagcacttt tttcaatgcg ctttccgcac cggtttccgt
     3121 gatgaccatg ttcagcgcgg cagagcgtcc gcggcggtac ttcttgtggt aattgtccag
     3181 caggttctgg tcgttcgtgt acgagtgaac ggtttcgatg tgtcccttct cgacgccgag
     3241 ctccttgtcc acgacataca ggatcggcat gatcgcgttc gtggtgcagg aagccgcaga
     3301 gaaaaccttg tcggaatccg gctttacggt ttcatggttg acaccgtaaa cgacattcgg
     3361 gatgtcgcct tttcccggcg cagtgagcag cactttcgcc acgccctttg ccttgagatg
     3421 gcggccgagt ccttcgcggt cgcggaaaac acccgtgttg tcgatgacga gcgcgttgtt
     3481 tatcccgtaa gccgtgtagt ccacatcttc cgggttcgct gcgtggatca tgtgcacgag
     3541 atgcccgttg atgagcagcg ccttgttctc aaggtcttcg atgatcgttc ctgggaaagg
     3601 tccgtgtacg gaatccatgc gcagcaggtc ggcgcgtttt acgatctcct catcgctgtt
     3661 cccgcgcgtt acgatcgcgc gcaggcgaag ctggtcgcct tttcccgcct gggaaataag
     3721 ctcgcgggca gcaagacggc cgatgcggcc gaaaccgaac agcacgacat ctttggggac
     3781 aatggatgct ttttccttcc cgatataatc cttcagcttg tccgtaacga acgctttcat
     3841 gtcggaataa tttccttttt cttccgtcca ttccgcgcag aggcggccga tgtcgatacg
     3901 cgaaggagcg agttccagcc tggtcagttc gcaggccagg ttggccgtat cgcggacatt
     3961 gatcgggcgc ttcacaatat cttttgcgta ctggtgcagg ttcatgatct cgctcgggct
     4021 gcggtcgatc atctggttgc ggaaaatgat cagttcggcg gatcgctcga accacaaggt
     4081 cccgatgtgc ccgatcagtt cgatcgcagc acgttcattg gaaatccagt cgttgagttc
     4141 gctttcgtaa gccttcgctt ttcctttctg aggctccatc gtttcgagtg tcatattgat
     4201 tccggttgtg tgattggtgg gtgtgaaata aaaaagcagc cgcaaagttc tttgatttca
     4261 tcgaaatccg accttgcggc tgctcgtaat ttttcaggac attttaaccc aggtaagctt
     4321 tcagaagttt actgcgggag ctgtgacgga ggcggcggat cgctttctcc ttgatctggc
     4381 ggacacgttc gcgcgtgaga tcgaatttcg caccgatctc ttcgagcgta agcccgtgct
     4441 ggcagccgat tccgaagaac agcttgatca catcacgttc gcgttcggta agcgtggcca
     4501 gtgaacgttc gatctctttc tggagcgatt cgttcatcag caggcggtcg gcacgcggtg
     4561 aatcgtggtt gacgatcacg tcgagcaggc tgttatcctc gccgttcagc agcggtgcgt
     4621 ccatggaaac atggcgaccg gaaacgcgca tcgtatctgc cactttatct tcgggcagat
     4681 cgagcacttg cgaaagctct tcggcggtag gttcacgttc gaattcctgc tcgaggcggg
     4741 aatacgcttt attgatctta ttcagcgaac ccacctggtt gagcggaaga cgcacgatac
     4801 ggctttgctc ggccaatgcc tggaggatcg actggcggat ccaccataca gcgtaggaaa
     4861 tgaatttgaa tccgcgggtt tcgtcaaaac ggcgagccgc tttaatgaga ccaagatttc
     4921 cttcgttgat aaggtcaggg agacttaaac cctggttctg gtattgcttc gaaacagaaa
     4981 cgacgaaacg aaggttagcc ttcaccatct ttgccaaagc ccgctcatct ccttcacgaa
     5041 ttttcttggc aagcactact tcttcttcag ctgtgatcag ctcttcacga ccaatctcct
     5101 ggaggtactt gtcgagcgaa gcgctttccc tgttggtgat tgatttcgtg atctttaatt
     5161 gt
//
LOCUS       DASTSF010000414         6700 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_492466,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000414 DASTSF010000000
VERSION     DASTSF010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 6700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6700
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_492466"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..866
                     /locus_tag="VFU15_11930"
     CDS             <1..866
                     /locus_tag="VFU15_11930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HEU4718539.1"
                     /translation="RKSAELDLRRQADVEAFFEKEKPEYVFLAAAKVGGIQANNIYRA
                     DFLYENIVIESNVIHAAYRSGVKKLLFLGSTCIYPKLAPQPLKEEYLLTGPLEPTNEP
                     YAIAKIAGIKMCDAYRDQYGCNFVSVMPTNLYGPNDNYDLNNSHVLPALLRKFHNAKK
                     AGSSSVVIWGSGTPLREFLHVDDMADACVHVMCTWNEKGFLNVGTGEDISIRDLALLI
                     KKVVGFGGELTFDASKPDGTPRKLTDVTRLHATGWKHKISLGQGIQSVYDDVKGLFDA
                     ELSGKHSLREV"
     gene            856..2676
                     /locus_tag="VFU15_11935"
     CDS             856..2676
                     /locus_tag="VFU15_11935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718540.1"
                     /translation="MRSEKNSNRGYYLRARAVVLVCLFSCGKIFSQQANVPLNYDWLQ
                     ESEAGMVKADGFPLSPSLMIYEADSALRHTFDSDDSSKVKMIDYVMVAAFPMQTSMRP
                     WIEQGHPLRKNVTLQNNFGYHYNPGFYETGQAMGFGKKLRRYLVNYHDQNSLLLVNRT
                     AKNGEPFFRLYIDPLLNVQYMNVSNDTSQSSFYINTRGITAYGDIGTKVSFETSFWEN
                     QAFFPQYISDYANTTQVVPGQGRWKTFRKTGYDYAMSTGYISYSPCRFFNVQAGHGKF
                     FVGDGYRSLLLSDNGFAYPFARFTGWFGPFQYTAIYASLMNLNVNAPVPPGTEHLYQK
                     KAASFYQMAMKLGRKGEISLFQGLIWTAADSSNRQCIRLAYVDPVMYTALPKYGLSSR
                     DNFLIGATFRYDLFHSLRLYGQWMADDLGKKGTVHNKTGFQLGAKYFNAFTLKHLHLE
                     IEYNRVNPYSYAATDPAQSYSQYNEPLAHPLGANFSEVIFSLYYKLGDFFLQARYSTA
                     MLGADSVSSDNGGQDIFKTDYLAYYPVRNFTLGQGQKTTITYFDVHAGYMISYASNLN
                     ISAGYTARNVSYGATSLPTNYVYVAVRTSLTNTYYDFFRK"
     gene            2750..3877
                     /locus_tag="VFU15_11940"
     CDS             2750..3877
                     /locus_tag="VFU15_11940"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HEU4718541.1"
                     /translation="MPHLLLTLFTAFLVVLMSTPSLIKVAFLKRLFDEPGDARKLHKR
                     VVPTIGGVIIFAATLFAFSLWFPYDQPSHEVLSRWVREFQFIVASLLILFFVGIKDDI
                     IGTAAIKKLGAHVIVALIIVIMADERIKSLHGLFGVNEIPLWASYLLSLFTFIVVINA
                     FNLIDGVDGLAAGVGLIGALSFGTLFWLGNDMVTAMIAYALAGSLFAFLFFNFSPAKI
                     FMGDSGSLSIGLIMAFLAIKYIEFVPAENTLPFVKELFTPVVAMSLIVYALTDTFRIF
                     VWRTMRGISPFSADRNHLHHLLIDSGFSHAKTVICIYISSISVFGMAVLTRGMAVTLS
                     FVITGAYVAVLMMIPWLIKKVSRNRNAAAGKTRELNPMEAA"
     gene            3979..5529
                     /locus_tag="VFU15_11945"
     CDS             3979..5529
                     /locus_tag="VFU15_11945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718542.1"
                     /translation="MPLNDDAATRIDFYCNNTDGHSSVKPLLDADLVPRDKYTGDSLP
                     AFRHSDHISERLAWRKYYQRDKVFTADRKWAFLLRPVYDFEGGKDLKANRTLYNTTLG
                     AVLDADYKQKIGVELRFAGGMSVLPNYLDSMAHFSGTMPGWSDRAYPGGNAQYAFQHF
                     SGNLIWRPSKVFNLQVGRDKHFWGDGYRSLFLSDLSAAYPYLQQQTTIWKLQYTSMFA
                     WLQDWTNSNYAAKNFRSKFATFHYLSFNAAKWLNLGVFESVVWQGADENRYRAFDPNY
                     LDPVVFFRPVEYSLGSSDNALLGFAFKVRFNKNNILYSQLILDEFYLKEIKNWSAGWW
                     ANKQGVQIGYKCYNFCGVERFFMQAELNIVRPYTYTHGSPQQNYANGFLPLAHPLGAN
                     FSELVGILSYSVKGFTVTGELVGARYGLDPAGKDYGQDIFISYIDRTKIDGEHTADYG
                     HKLWDGIATNMFYAEFKASYRFNTAFPLRVELIAGARSESNVNVTKKSSYVMAGLSLP
                     LFRTYRDY"
     gene            5712..6578
                     /gene="cyoE"
                     /locus_tag="VFU15_11950"
     CDS             5712..6578
                     /gene="cyoE"
                     /locus_tag="VFU15_11950"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009580636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HEU4718543.1"
                     /translation="MNKLADYAQFAKLRLSSLVVFSAVIGYVIGVRELDHESSWVELT
                     ALILGGFLVTGSSNGFNQVIERETDKLMSRTMNRPLPTGRMSVREGVVLASVMGVAGI
                     LLLALFTNMLSAILGFLALILYTLVYTPSKKRTPFATFIGAFPGAIPPMLGWVAATNG
                     FGMIGTSAMLLFAIQFMWQFPHFWAIAWVSHDDYTRAGFRMLPSAGGRDKASAFQVVV
                     YTLGLIPVSLTPLLFRMCGTVAGVVILACGVAFLVQAMTLYRNCDVKSASRLMFGSFI
                     YLPVVQLALLFG"
BASE COUNT         1495 a         1828 c         1719 g         1658 t
ORIGIN      
        1 cgcggaaatc agccgagctc gatctgcgcc gccaggcgga cgttgaagcg ttttttgaga
       61 aggaaaaacc ggaatatgtt ttcctcgctg ctgccaaagt aggcggtatc caggccaaca
      121 acatttaccg cgcggatttt ctctatgaaa atatcgtcat cgaatcgaac gtcattcatg
      181 cggcgtacag gagcggcgta aaaaaattgc tgttcctcgg ctccacctgc atttacccga
      241 agctcgcgcc ccagccgctg aaggaagaat acctgctgac gggtccgctc gagccgacga
      301 acgaaccgta tgccatcgcc aagatcgccg ggataaaaat gtgcgacgct taccgcgacc
      361 agtacggctg caatttcgtt tccgtcatgc cgacaaacct ttacggaccg aacgacaatt
      421 acgatctgaa taattcgcac gtgcttccgg cgctgctgcg gaaatttcac aatgcgaaaa
      481 aggcaggttc ctcttccgtc gtgatttggg gaagcggaac gccgttgcgc gaatttctcc
      541 acgtcgacga catggccgat gcctgtgtgc atgttatgtg cacgtggaac gaaaaaggat
      601 ttctcaacgt cggcaccggc gaagacatca gcatccgcga tcttgcgctg ctgattaaaa
      661 aggtcgttgg cttcgggggc gagctgacgt tcgacgcttc gaaacccgac ggaacaccgc
      721 gcaagctcac ggacgttacg cggctgcacg ccacggggtg gaagcataag atttcgctcg
      781 ggcagggaat ccaaagcgtg tacgatgacg taaaaggcct gttcgatgct gaactttccg
      841 gaaaacattc cctgcgtgag gtctgaaaaa aacagtaacc gcggttatta cctgcgcgcg
      901 cgcgctgtcg tgcttgtttg tcttttttcc tgcggaaaaa tcttttccca gcaggcgaat
      961 gttccgctga attacgattg gctgcaggaa agcgaggccg ggatggtgaa agcagacggg
     1021 ttcccgcttt ctccttcgct gatgatctac gaagcggatt cggcgctgcg ccatacgttt
     1081 gacagcgacg attcttcgaa agtgaaaatg atcgattatg taatggtcgc tgcatttccc
     1141 atgcagacgt ccatgcgccc gtggatcgaa cagggacacc cgctgcggaa gaatgtgacg
     1201 ctgcaaaata atttcggtta tcattataac cccggttttt atgaaaccgg ccaggcaatg
     1261 ggctttggaa aaaagctccg gagatacctg gtcaattatc atgatcaaaa cagccttctc
     1321 ctggtaaatc gcactgcgaa gaacggcgaa ccgtttttcc gcctgtatat cgatccgttg
     1381 ctgaatgttc aatacatgaa tgtcagcaac gatacatcgc agtcttcttt ttatatcaat
     1441 acacgcggca tcactgcgta tggcgacatc ggtacgaaag tttcatttga aacgtcgttc
     1501 tgggaaaacc aggcgttctt cccgcaatac atttcggatt acgccaacac gacgcaggtc
     1561 gttcccggcc agggaagatg gaagacattc aggaaaacgg gttatgacta cgccatgtcc
     1621 acaggataca tttcgtattc accctgccgt tttttcaacg tacaggccgg ccacggaaaa
     1681 tttttcgtcg gcgacggcta tcgctccctg ctgctttcgg ataacggctt cgcatatcct
     1741 ttcgcacgtt tcaccggctg gttcggtccg ttccagtaca ccgcgatcta cgcttcgctg
     1801 atgaacctga acgtgaatgc gcccgttccg ccgggaaccg aacatctcta ccagaagaaa
     1861 gccgcgtcgt tttaccagat ggcgatgaag ctgggacgta aaggagaaat ttccctgttc
     1921 cagggactga tctggaccgc agccgacagc agcaaccggc agtgcatccg cctcgcttat
     1981 gttgatcccg ttatgtacac ggcgcttccg aaatacggcc tcagcagccg ggataatttc
     2041 ctcatcggcg ctacattccg ttatgatctt tttcattcgc tccgtttgta cggccagtgg
     2101 atggcggatg atctcggcaa aaaaggaacc gtgcacaaca aaacaggttt ccagctcggc
     2161 gcgaaatatt tcaacgcgtt tacgctgaag catcttcacc tggagatcga atacaaccgc
     2221 gtcaatccct attcctatgc tgcaacagat ccggcgcaat cgtattcaca gtacaacgaa
     2281 ccgctggcgc acccgctggg cgcaaatttt tccgaagtga tcttttcgct gtattataag
     2341 ctcggtgatt ttttcctgca ggcgcgttat tccaccgcga tgcttggtgc agattccgta
     2401 agcagcgaca acggcgggca ggatattttc aagaccgatt acctcgcgta ttatcccgtc
     2461 aggaatttca cccttggcca gggacagaag acgacgatca cgtacttcga cgtgcatgcc
     2521 ggctatatga tcagttatgc gagcaatctc aatatttctg cgggatatac agcgcgaaat
     2581 gtgagctatg gcgcaacctc cttacccacg aattacgtat acgtggcggt ccggacttcc
     2641 ttaacgaaca cttattacga tttcttccgg aaataattac ttttgttgaa aacctgttag
     2701 ttgcttgttt ataaccttgg cggccaaccg gttaaagtac accagatcaa tgccgcacct
     2761 gctacttacg ctttttactg ctttccttgt ggttctgatg tccacgcctt cactgatcaa
     2821 ggtggctttt ctcaagcgcc tgttcgatga gccgggtgat gccaggaagc tgcacaaacg
     2881 ggtggttccc accatcggcg gtgtgatcat tttcgcggcc acgcttttcg ctttttcact
     2941 ctggttcccg tacgatcagc ccagccatga agtgctgagc cggtgggtgc gtgaattcca
     3001 gttcatcgtg gccagtctcc tcatcctctt tttcgtcggg ataaaagatg acatcatcgg
     3061 cacggcggcg atcaagaaac tcggcgcgca cgtgatcgtt gcgctcatta tcgtcattat
     3121 ggcggatgaa cgcataaaaa gcctgcacgg gttgttcggt gtgaatgaaa ttccgctctg
     3181 ggccagctac ctgctttcgc ttttcacatt catcgtggtc atcaatgcgt tcaacctgat
     3241 cgatggcgtc gacgggcttg ccgccggcgt cgggctcatc ggggcgctgt cgttcggaac
     3301 gcttttctgg ctgggcaacg acatggtcac ggcgatgatc gcctatgcgc tggccgggtc
     3361 gcttttcgct ttcctgttct tcaacttttc acctgcgaag atcttcatgg gcgattccgg
     3421 ctcgctgagc atcggcctta tcatggcttt tctcgccatc aaatacatcg aattcgttcc
     3481 ggccgaaaat acactgccgt tcgtgaagga gctgttcacc ccggtcgtcg ccatgtcgct
     3541 catcgtttac gcgctcaccg atacgttccg catcttcgtg tggcgcacca tgcgcggcat
     3601 ctcgcctttt tccgccgacc gcaaccacct gcatcacctg ctcatcgaca gcggattttc
     3661 acatgcgaaa acagtgatct gcatttacat ctcgagcatt tccgttttcg gaatggccgt
     3721 cctcacccgc gggatggcag tgacgctttc cttcgtcatc accggcgctt atgtcgccgt
     3781 actgatgatg atcccctggc tcatcaaaaa agtgagccga aacaggaacg ccgctgccgg
     3841 gaaaacacgg gaactcaacc cgatggaagc ggcatgaggc ttacgattta cgatttgcga
     3901 tcaacactcc tccgccggcc ggtcgcatcg ctttttctct tttgtttttt cacaacacct
     3961 tttttcgccc agcatacaat gccgctcaat gacgatgcgg cgacgaggat cgatttctac
     4021 tgcaacaata cggacgggca cagttccgtg aaaccgctcc tcgatgcgga tcttgttccc
     4081 cgtgataaat acaccggcga ttcgctgccg gctttccggc acagcgatca catttcggaa
     4141 agactggcgt ggagaaaata ttaccagcgt gataaagttt ttacagctga ccggaaatgg
     4201 gcattcctcc ttcgcccggt ttatgatttc gaaggcggga aagacctgaa ggcgaaccgc
     4261 accctttata acacaacgct cggcgctgtt ctcgacgcag attacaaaca gaagatcggt
     4321 gtcgaattgc gttttgccgg cggcatgagc gttctgccaa actacctcga ttccatggca
     4381 catttttccg ggacgatgcc gggctggagc gaccgcgcgt atcccggcgg aaatgcgcag
     4441 tacgccttcc agcatttttc aggaaacctc atctggcggc cgtcgaaagt gttcaacctg
     4501 caggtcgggc gcgacaagca tttctggggc gacggttacc gctcgctttt tctttccgat
     4561 ctcagcgcag catatcccta tctacagcag cagaccacga tctggaaact gcagtacacg
     4621 tcgatgtttg cgtggctgca ggactggacg aattcaaatt acgcagcgaa aaatttccgg
     4681 agcaagttcg ccacgttcca ttacctcagc ttcaatgcag caaagtggtt gaatctcggc
     4741 gtgttcgagt ccgttgtctg gcagggcgcc gatgagaacc gctaccgcgc attcgatccg
     4801 aattacctcg atcccgtcgt tttttttcgc ccggtcgaat acagtctcgg ctcttcggat
     4861 aatgcgttgc tcgggtttgc gttcaaagtg cgtttcaata aaaacaacat tttgtattcg
     4921 cagctgatcc tcgacgagtt ttacctgaaa gagatcaaaa actggagcgc cggctggtgg
     4981 gccaacaagc agggcgtgca gatcgggtac aaatgctaca atttctgcgg agtggaacgc
     5041 ttcttcatgc aggccgagct gaacatcgtg cggccgtaca cgtacacgca tggaagtccg
     5101 cagcagaatt acgcgaacgg atttctgccg ctggcgcatc cgctcggtgc gaatttttcg
     5161 gagctcgtgg gcattctttc gtattccgta aaaggattca cggttaccgg cgagcttgtg
     5221 ggcgcgcgtt acgggctcga tcctgcggga aaagattacg gccaggatat tttcatttcc
     5281 tacatcgacc ggacgaagat cgacggggag cacacggccg attatggcca caagctgtgg
     5341 gacggcattg cgacaaacat gttctacgcg gaattcaaag catcgtaccg gttcaatacc
     5401 gcattcccgc tccgtgtcga actgatcgcg ggagcacgtt cagagagtaa cgtgaacgtg
     5461 acgaaaaaat cttcttacgt catggccgga ctcagtctcc cgcttttccg cacctatcgc
     5521 gattactgat agctgaacat ggcggttgga aatcgaaaaa cacctgttca tcagcaatta
     5581 tttagaagta atctaaattc tgtacgggcc ggaactttgt aagcggcgga atacctaaat
     5641 ttgcccgttt taaaacagcc caaaaattgc ttgcttcccg ggcacataca atcgacaaac
     5701 ctgccagtgc aatgaataag ttggctgact atgctcagtt cgctaaactg cgcttgtcct
     5761 cgctggtagt tttttccgca gtgatcggtt acgtgatcgg cgtacgtgag ctggaccacg
     5821 aatccagctg ggtcgagctg accgcgctta tcctcggtgg cttcctggtc acgggatctt
     5881 ccaacggttt caaccaggtc atagagcgcg aaaccgacaa actcatgtcg cgcacgatga
     5941 accgccctct tcctacaggt cgcatgagcg tgagggaagg cgtggtgctt gcttcggtaa
     6001 tgggcgtcgc gggcattctt cttctcgctt tgtttaccaa catgctcagc gcgatcctcg
     6061 gatttcttgc cttgatcctt tacacgctgg tctacacgcc ctccaaaaaa agaacacctt
     6121 tcgccacgtt catcggtgct ttccccggtg ccattcctcc catgctggga tgggtggcag
     6181 caacgaacgg cttcgggatg atcggaacat cggccatgct gctgtttgcg atacagttca
     6241 tgtggcagtt cccgcatttc tgggccattg cgtgggtttc gcacgatgat tacacccggg
     6301 cgggtttccg catgcttcct tccgcaggag gaagagataa ggccagcgcg ttccaggtgg
     6361 tggtttatac gctcgggctt atccctgtca gcctgacgcc gcttcttttc cgcatgtgcg
     6421 gaaccgttgc gggagtggtc attcttgcct gcggggttgc tttcctggtg caggccatga
     6481 cattataccg caactgcgac gtgaaatcgg catcgcgtct catgttcgga tcgttcatct
     6541 acctgccggt agttcagctc gcacttttat tcgggtaaac agatggaagc agccatggca
     6601 acaagcagtg aagaacgcgc aacccgtgaa aaaggccaga aaaacctttt gtatctcggg
     6661 ctcttcagta ttatcatgct gttcgccggt cttaccagcg
//
LOCUS       DASTSF010000415         9799 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1819262,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000415 DASTSF010000000
VERSION     DASTSF010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 9799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9799
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1819262"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..950
                     /locus_tag="VFU15_11955"
     CDS             <1..950
                     /locus_tag="VFU15_11955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010992214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HEU4718544.1"
                     /translation="WKGIAQDAIVMNTDDLLCVGATNNILLSSTIGRNKNRIPGEVIS
                     ALINGTEEVLAELREMGVGIRSTGGETADVGDLVRTVIVDSTVVARMKRSEVITNEKI
                     SPGDVIVGLASSGKATYEAEYNGGMGSNGLTSARHDVFAHELAAKYPESYDPEVPEDL
                     VYAGKIKLTDLVDIGDGKKIPAGKLVLSPTRTYAPVITRILDELRAEIHGIVHCSGGA
                     QTKVLHFVNDVHVIKDNLFPVPPLFRLIQEQSGTPWSEMYKVFNCGHRMEIYLPENFA
                     QRVIGISKQFNIPAQIIGRVEKASGRKLTIRSEYGEFGY"
     gene            1025..2098
                     /gene="prfA"
                     /locus_tag="VFU15_11960"
     CDS             1025..2098
                     /gene="prfA"
                     /locus_tag="VFU15_11960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010599269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEU4718545.1"
                     /translation="MLEKLQSIETRFHEVEQQLSSPDVMSDMKRFAQLNREYKELKNI
                     VEHYHEYRNVVGNISSAKNVLATEKDPDFLDMAKAELLELEAKKERMEEQIKNLLIPK
                     DPEDARNAVIEIRSGTGGDEAAIFAGDLWRMYTRYCEMRGWKTEVVDFSEGTMGGYKE
                     VVMNVIGDNAYGILKYESGVHRVQRVPETETQGRVHTSAATVVVLPEADDVDVVINPA
                     DIEITTARSGGAGGQNVNKVETKVILYHKPSGIQIACQVERSQHANRERAMQMLKTKL
                     FEIEYQKKMEAESKKRKTMVSTGDRSAKIRTYNYPQSRVTDHRIGLTQHNLPAFMNGD
                     IQSMIDALQVAENAERLKEGVGS"
     gene            2206..2862
                     /locus_tag="VFU15_11965"
     CDS             2206..2862
                     /locus_tag="VFU15_11965"
                     /inference="COORDINATES: protein motif:HMM:NF019958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEU4718546.1"
                     /translation="MEAHTDRKDHLLDIAEELFSENGFDATSVRALAERAGMNVAMVS
                     YYFGSKEKLFEAVIIRKTAYIREKLMLLAGDKSLSAWEKMRELIGNYTERILNDEGRY
                     HKMMMRELSLRKRTGLSSVIEEKIMTNMNAVYSIVKEGIKKGEFRKDVDFTLMMSTIF
                     GTLTHSTTSSNLLPRFFEVEKRKPKITKQDYVTLMKKHLEQLIGRLLLVHPEKFLKKK
                     "
     gene            2894..4222
                     /locus_tag="VFU15_11970"
     CDS             2894..4222
                     /locus_tag="VFU15_11970"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEU4718547.1"
                     /translation="MKFIAFTGILLIAAGGAFAQNQAGSQTLNIGQAVQMGVANSKQL
                     KLDTLKLAQVEAKQAQVSDAALPNVSLNAGYSRLSPIDPVTFQFPGSPEPVTLFPVIL
                     NNYTTRASVSEGIFTGWRLKYTEESYGFINKAAQLDVTKDEQEVRLNIMSAYISYVKL
                     TLSQHIVEENLKTAQQRVDEVTSMKERGLATDNDVLKAQLYQSNLELSKSDVDNSIAV
                     AQFNLCILLGLPEGTTINADTTGLFAAVNLLPETQYEQDAVTNRSELKSAGYRVQSSQ
                     SNVKLAQASYYPTVGVGADYYFARPNQRIIPYVDEFRATWDVGVNVSWNISSLYTGKH
                     NVQEAQVQLMQTQVQSDMLTDNIKMEVFQNYMACQGAITKMSMLQLAVTQAEENYRQT
                     KARYDQQLSLMSEVLDADAALLQARINLVLQQADVQLSYDKLLKSTGTLK"
     gene            4241..5320
                     /locus_tag="VFU15_11975"
     CDS             4241..5320
                     /locus_tag="VFU15_11975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017257957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEU4718548.1"
                     /translation="MEQNQQAEAPQPEKKKKRKIAPFILGALVLAGGTYAVRTFIFNQ
                     HHETTDDAQLEGSISPVLPRVSGYVTEVRFEDNQRVHKGDTLVVLDDRDMKIKELQAE
                     AALDNAKANVEVVKANISTAEASVATAKSNVDLAKVKVDKAAQDYDRYSALLNQKATT
                     QAQFDGVRSERDASLSQYDVAKKQYDAAAKQMLAAEGQVKVAESMVAQRQADVDYAKL
                     QLSYAVITAPMDGVVSKKNVEPGQFIQAGQALFSVVDDSGVWVVANFKETQLEKMLVG
                     QSVDVHVDAFGDTDIKGEVASFSGATGARFALLPPDNATGNFVKVIQRVPVKIKLNVD
                     KAMADKLRPGMSVSVVVNIDDPGKQ"
     gene            5455..7011
                     /locus_tag="VFU15_11980"
     CDS             5455..7011
                     /locus_tag="VFU15_11980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010599337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HEU4718549.1"
                     /translation="MAEKGFAKWIIVITVILAALLELIDSTVVNVALNQIMGNLGATL
                     EDVAWVVTAYAVANVIILPMNGWLSARFGRKNYFAFSIILFTASSFLCGNAHNIWELI
                     FFRFIQGIGGGALLSTSQSILFETFKPEERGMATAIFGIGVVLGPTFGPTLGGWITDH
                     YSWPWIFYINVPLGIIAAILTITYIQNSTLHTKPPKTDWIGIALLVAGIASLQIVLER
                     GASEDWFETRYITVLAAVAAFSLITFVWWELRTEHPVVNFQVLIGSRQLAFGMMFTFI
                     LGFGLYASLFIFPIFVQGLLGWTAEQTGLLLMPSGLVSLFVMPTVGVLLRKGIPPQYL
                     ATIGFIMFFIFTHVLSVSTLASGWSDFMWPQLLRGIGMGFMFVPITNFALGGLAQKDI
                     HQGTGLNNMMRQLGGSFGIAAVATFINNRTAFHRATLLQNINIYDTETQQRLKMFTGA
                     FMGHGYDHNTAQTMAAKALDGAVIRQSMLLSYMDNFWIVGIFFLCCIPLLIFQPKTKQ
                     VAKISAADAH"
     gene            7051..7863
                     /gene="pyrF"
                     /locus_tag="VFU15_11985"
     CDS             7051..7863
                     /gene="pyrF"
                     /locus_tag="VFU15_11985"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007900485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEU4718550.1"
                     /translation="MTKAELVKQIRGKKSFLCIGLDTDSKKIPKHLLKYEDPVFEFNK
                     QIIGATHDLCVAYKPNLAFYEADGVKGLQSLEKTMKVIPQECFTIADAKRGDIGNTSA
                     MYARAFFDHYHFDSVTVAPYMGSDSVKPFLGRENKWVILLALTSNEGAMDFETQKLAD
                     GKTLYEEVLRRSQEWGNAENMMYVVGATRAEYLAEIRKIVPDHFLLVPGVGAQGGSLS
                     DVAKYGMNKECGLLVNASRSVLYASDGEDFAEKAREEAMRMREEMKTLLMDN"
     gene            complement(8027..8632)
                     /locus_tag="VFU15_11990"
     CDS             complement(8027..8632)
                     /locus_tag="VFU15_11990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718551.1"
                     /translation="MQSLTSLPDWITALIGGFVTFAAKTAYDKFAKRKENKENELEKL
                     HQLQEILREGKSTLHSQNYLRARLNRSLEERFDRGGLGYNQFFAKHFSELTPEEKTLF
                     DLIRGISENSMYSANKRLLEWANAQAGMFAMKTGDAALNDFRKDLSEMKDHLVIWMDK
                     YSSIFKKDPAQCLVYLKDEEAHGKGFPQRIEVSLATLISKM"
     gene            complement(8959..9522)
                     /locus_tag="VFU15_11995"
     CDS             complement(8959..9522)
                     /locus_tag="VFU15_11995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020403616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HEU4718552.1"
                     /translation="MPEKENPKLKTLVPWLYHNDHHNTVRYILGTEGKRPLVCIGINP
                     STAEPDRLDNTLKSVARLAKSNGFDSWIMLNVYPQRATDPDHLHRKADAVIHRKNLAA
                     IEKMLSGIKGRPVIWAAWGTLIDKRDFLSQCLRDIYHVAKKHHCRWVTIGGVTKDGHP
                     RHPLRMSAATKTTPFAIDDYMKRVENW"
BASE COUNT         2399 a         2383 c         2836 g         2181 t
ORIGIN      
        1 tgtggaaagg aattgcccag gatgcaatcg tcatgaatac ggacgatctc ctgtgcgtgg
       61 gagcaacaaa caatatactg ctgtcctcca ctatcggccg gaataaaaac cgaattccgg
      121 gcgaggttat atccgcgctg atcaacggaa cggaagaggt gctcgctgaa ttacgggaga
      181 tgggagtcgg gattcgcagc acaggcggag aaacagccga tgtgggcgat cttgtgcgga
      241 cggtgatcgt cgattccacg gtggtggcgc gcatgaagcg cagcgaggtg atcacaaatg
      301 aaaaaatttc gccgggtgat gtgatcgtgg gacttgcttc cagtggtaag gcgacgtacg
      361 aagcggaata taacggggga atgggaagca acgggctcac gtccgcgcgg cacgatgtgt
      421 ttgcgcatga gctcgccgcc aaatacccgg agagctatga tccggaagtg ccggaagatc
      481 ttgtttacgc gggaaaaatc aagctgaccg acctggtcga catcggtgac ggaaagaaaa
      541 ttccggcagg caagctcgtg ctttccccga caaggaccta tgcgccggtg atcacgcgta
      601 ttctcgatga gcttcgtgcg gaaatacacg gcatcgttca ttgcagcgga ggtgcgcaga
      661 cgaaagtgct gcattttgtg aatgatgttc acgtgataaa ggacaacctg ttccctgttc
      721 ccccgttgtt ccggctcata caggaacaga gcggaacgcc gtggagcgaa atgtacaaag
      781 tgttcaattg cggtcatcgt atggagatct acctcccgga aaatttcgcg cagcgcgtga
      841 tcggaatctc caaacagttc aatatcccgg cgcagatcat cgggcgggtg gaaaaagcct
      901 ccggcaggaa gctcaccatc cgcagcgaat acggagagtt cggttactag cttcagtcag
      961 gccgttcctt cgtcatctct ttttctttat tcatttcatg taaaattcaa caacgggcat
     1021 ccagatgctg gaaaaactgc aatcgataga aacacgtttc cacgaagtgg aacaacagct
     1081 ttcttctccc gacgtcatgt cggacatgaa aagattcgcg cagctgaacc gggaatacaa
     1141 agagctgaaa aatatcgtgg agcattacca cgaataccgc aatgttgtcg gtaatatttc
     1201 ttcggcgaaa aacgtgctgg cgacggaaaa agatcccgat ttcctggata tggcgaaggc
     1261 ggaattgctg gagctcgagg cgaagaaaga acgcatggag gagcagatca aaaacctgct
     1321 tatcccgaaa gacccggaag atgcgcggaa tgcggttatc gagattcgtt caggtacggg
     1381 cggtgacgag gcagctattt tcgcgggcga tctctggaga atgtacacac gttactgcga
     1441 aatgcgggga tggaaaaccg aggtggtgga tttcagcgag ggaacgatgg gcggttacaa
     1501 ggaagtggtg atgaatgtga tcggggataa tgcgtacgga attctgaaat acgaatccgg
     1561 cgtgcaccgt gtgcagcgcg ttcccgaaac ggaaacgcag ggccgcgtgc atacgtcggc
     1621 ggcaacggtt gtcgtgcttc cggaggcgga tgatgtcgat gttgtcatca atcccgcgga
     1681 catcgagatc actaccgcgc gttcgggcgg agcgggcgga cagaacgtga ataaagtcga
     1741 aacgaaggtc attctctatc acaagccgtc ggggatacag atcgcctgcc aggtggaacg
     1801 ttcgcagcat gccaaccgcg agcgcgcgat gcagatgctg aagacgaaac tgttcgagat
     1861 cgaataccag aaaaagatgg aagccgaatc gaagaaaaga aaaacaatgg tttccaccgg
     1921 cgaccgttcg gctaaaatac gcacgtacaa ttatccgcag agccgtgtca ccgatcaccg
     1981 catcgggctg acgcagcaca accttcccgc tttcatgaac ggcgatatcc agagcatgat
     2041 cgatgcgctg caggtggccg agaatgcgga acgactgaaa gaaggcgtcg gcagctgagc
     2101 cggcaatcct attttatcgc gctgttcctg agtgacttaa caaaagaatt aaacaagcgt
     2161 ttgaatgtta aacaaacgtt taattagctt tgtcgcgttt ttcacatgga agcacataca
     2221 gacagaaaag atcacctgct ggacattgcc gaagagctgt tttccgaaaa cggtttcgac
     2281 gcaacatccg tgagggcgct ggcggaaaga gccgggatga acgtggcgat ggtctcctac
     2341 tatttcggct ccaaggaaaa actgttcgag gcggtgatca tccggaagac ggcttacatc
     2401 cgcgagaagc tgatgctgct ggccggcgac aaatcgcttt ccgcctggga aaaaatgcgc
     2461 gagctgatcg gtaattacac ggaacggata ctgaacgacg aaggccgtta ccacaagatg
     2521 atgatgcgcg agctttcact gcggaaacgt acgggattgt cgagcgtgat cgaggaaaag
     2581 ataatgacga acatgaacgc ggtttacagc atcgtgaaag agggaataaa aaaaggcgag
     2641 ttccggaaag acgtggattt caccctgatg atgagcacga tcttcggaac actgacgcat
     2701 tcgaccactt ccagcaacct gctcccgcga tttttcgaag tggaaaaacg gaaaccgaag
     2761 atcacgaagc aggattacgt gacgctgatg aaaaaacacc tggagcagct gatcggccgc
     2821 ctgctgctgg tgcatcccga aaaattcctg aagaaaaaat aaaaaaacag ttcaaccgac
     2881 tcaataagaa aaaatgaaat tcatcgcatt cacagggatc cttctcatcg cagccggcgg
     2941 agcttttgcg caaaaccagg cgggctcgca aaccctgaac atcgggcagg ccgtgcagat
     3001 gggcgtcgcc aacagcaaac agctgaagct ggacacgctg aaactcgcgc aggtggaagc
     3061 gaagcaggcg caggtgagcg atgcggcgct cccgaacgta agcctcaatg cgggctattc
     3121 gaggttgagc ccgatcgatc ccgtaacgtt ccagtttccc ggcagcccgg aacctgtgac
     3181 gctcttcccg gtgatcctga acaactatac gacgcgggct tcggtgagcg aaggaatttt
     3241 caccggctgg cgcctgaaat acacggagga atcgtacggc ttcatcaaca aggcggcgca
     3301 gctcgacgtg acgaaggacg agcaggaagt gcggctcaac atcatgtcgg cgtacatctc
     3361 gtacgtgaag ctcacgcttt cgcagcacat cgtggaggaa aacctgaaaa cggcgcagca
     3421 gcgcgtggat gaagtgacga gcatgaaaga gcgcggtctt gcgacggaca acgacgtgct
     3481 gaaagcgcag ctgtaccagt cgaacctcga gctgtcgaaa agcgacgtgg acaacagcat
     3541 cgccgtcgcg cagttcaatc tctgcattct gctcggtctt ccggaaggaa cgacgatcaa
     3601 tgccgacacg acaggactgt ttgccgctgt gaacctgctg ccggaaacac aatacgagca
     3661 ggatgcggtg acgaaccgga gcgagctgaa atcggcaggt taccgcgttc agtcttcgca
     3721 gagcaacgtg aagcttgcgc aggcttccta ttatcccacg gtcggtgtgg gcgcggatta
     3781 ttatttcgca cggccgaacc agcgcatcat tccgtacgtg gacgaattcc gtgcgacgtg
     3841 ggatgtgggc gtgaacgttt cgtggaatat ctcttcactt tatacgggca agcacaacgt
     3901 gcaggaagcg caggtgcagc tcatgcagac gcaggtgcag agtgacatgc tcacggacaa
     3961 tatcaaaatg gaagttttcc agaactacat ggcctgccag ggggcgatca cgaaaatgtc
     4021 gatgctgcag ctggctgtca cgcaggcgga ggaaaattac cggcagacaa aagcgcgtta
     4081 cgaccagcag ctttcgctga tgagcgaagt gctggatgcg gatgcggcgt tgctgcaggc
     4141 aaggatcaac ctcgtgctcc agcaggcgga cgttcagctt tcctacgaca agcttcttaa
     4201 atcaacggga acacttaaat aagcaaacag tatttataaa atggaacaga atcagcaggc
     4261 ggaagctccg caaccggaaa agaaaaagaa aagaaagatc gctcctttca ttcttggtgc
     4321 gctggtcctc gcgggaggaa cctacgctgt ccgcacattc attttcaacc agcatcatga
     4381 aacaacggac gatgcgcagc tcgagggaag catcagtccc gtgctgccgc gcgtttcggg
     4441 ctacgtgacg gaagtccgtt tcgaagacaa ccagcgcgtg cacaaaggcg atacgctggt
     4501 ggtgctcgac gaccgcgaca tgaaaataaa agagctgcag gcggaagccg cgctcgacaa
     4561 cgcaaaagcc aacgtcgaag tggtgaaagc gaatatttcc accgcggaag catctgtggc
     4621 gacggcgaag tcgaacgtgg acctggccaa agtgaaagtg gacaaggcgg cgcaggatta
     4681 cgatcgttac agcgcgctgc tgaaccagaa ggcgaccacg caggcgcagt tcgacggcgt
     4741 gcgttcggag cgtgacgctt ccttgtcgca atacgacgtg gcgaagaaac agtacgacgc
     4801 ggcggccaag caaatgctcg cagcggaagg acaggtgaaa gtagccgagt cgatggttgc
     4861 gcagcggcag gctgacgtcg attatgcgaa gctgcaattg tcttatgccg tgatcaccgc
     4921 accgatggat ggcgtggttt cgaagaagaa cgtggagccg ggacaattca tccaggcggg
     4981 acaggcgttg ttttccgtag ttgatgattc aggtgtgtgg gtagtggcga acttcaagga
     5041 aacgcagctg gaaaaaatgc tcgtcggtca atcggtggat gtgcacgtgg acgctttcgg
     5101 cgatacggac atcaaaggag aagttgcttc gttctcgggt gcgacgggtg cgcgcttcgc
     5161 gcttctgcct cctgacaacg caacggggaa tttcgtgaag gtgatccagc gtgtgccggt
     5221 gaaaataaag ctgaacgtgg acaaggcgat ggcagacaaa ctgcgcccgg gaatgagcgt
     5281 gagcgtcgtg gtgaacatcg acgacccggg gaaacaatga agaactgacg attgacaggt
     5341 gatgaatggc cccgggtgag gcggcatcct gccggaggca gataaagccg aacacgggaa
     5401 gtgcgggaat aaaatgaact gaaggtgcgc cccggaaaaa ggaaaaaata aaaaatggca
     5461 gaaaagggtt ttgcaaagtg gatcatcgtg atcaccgtta tcctggcggc gctgctcgaa
     5521 ctcatcgatt cgacggtggt gaacgtggcg ctgaaccaga tcatgggtaa tctcggtgcg
     5581 acgctcgaag acgtggcatg ggtggtgacg gcgtacgctg tggcgaacgt gatcatcctc
     5641 ccgatgaacg gttggctgtc cgcacgtttc ggacgaaaaa attatttcgc gttctcgatc
     5701 atcctgttta cggcgtcgtc gtttctctgc ggcaatgcgc acaacatctg ggagctgatc
     5761 tttttccgtt tcatccaggg catcggcggc ggcgcgctgc tgtcgacctc acaatcgata
     5821 cttttcgaaa cgttcaagcc ggaagagcgc ggcatggcca cggcgatctt cggcatcggc
     5881 gttgtgctcg gcccgacatt cggtccgacg ctcggcggct ggatcaccga tcactattcc
     5941 tggccgtgga tcttttacat caacgttccg ctcggcatca ttgcggcgat acttaccatt
     6001 acgtacatac agaattcgac gctgcatacg aagccaccga aaacggactg gataggaata
     6061 gcgctgctgg tggcgggcat tgcttcgctg cagatcgtgc tggagcgcgg cgcttcggaa
     6121 gactggttcg agacgcgcta catcaccgtg ctggctgcgg tcgccgcatt ttcgctcatc
     6181 actttcgtgt ggtgggagct gcggacggag cacccggtgg tgaatttcca ggtgctgatc
     6241 gggagccggc agctggcgtt cgggatgatg ttcacgttca ttctcggttt cggattatac
     6301 gcgtcgctgt tcatctttcc cattttcgtg cagggacttt taggctggac ggcggagcag
     6361 accggtttgc tgctgatgcc gtcgggactg gtttcgctct tcgtgatgcc gaccgtcggc
     6421 gtgctgctgc gcaaaggaat tccaccgcaa tacctggcga cgatcgggtt catcatgttc
     6481 ttcatcttca cgcacgtgct cagcgtctcg acgctcgcat cgggctggag cgatttcatg
     6541 tggccgcagc tgctgcgcgg gataggaatg ggattcatgt tcgtgccgat cacgaatttt
     6601 gcgctgggcg gactggcgca gaaagacatt caccagggga cagggctcaa taacatgatg
     6661 cgccagctcg gcggttcgtt cggcattgcg gccgtggcga cgttcatcaa caaccgcacg
     6721 gcgttccatc gtgctacgct gctgcagaac atcaacattt acgatacgga aacacagcag
     6781 cggctgaaaa tgttcaccgg tgcgttcatg ggccatggct acgatcacaa cacggcgcag
     6841 acgatggctg caaaggcgct cgacggagcg gtgatccgcc agtcgatgct gctttcgtac
     6901 atggataatt tctggatcgt gggcatcttt ttcctgtgct gcataccgct gcttattttc
     6961 cagccgaaaa caaagcaggt ggcgaagatc agcgcggcgg acgcacatta aaattccgaa
     7021 tgcttaattt tggatcctta accaggcagg atgacaaaag cagaattagt aaaacagatc
     7081 cgcgggaaga aaagttttct ctgcatcggg ctggataccg acagcaaaaa aattccgaag
     7141 catttactga agtacgaaga tcctgttttt gaattcaaca agcagatcat cggagcgacg
     7201 cacgatctct gcgtagcgta caagcctaat cttgcattct acgaagccga cggcgtgaaa
     7261 ggactgcagt cgctggagaa aacaatgaaa gtcatcccgc aggaatgttt cacgatcgcc
     7321 gacgcaaaac gcggggacat cggcaacaca tcggcgatgt atgcgcgtgc attctttgat
     7381 cattatcatt tcgattctgt caccgttgcg ccttacatgg gaagcgattc ggtgaagccg
     7441 ttcctcggtc gcgaaaataa atgggtcatt cttcttgcgc tgacaagcaa tgaaggcgcg
     7501 atggatttcg aaacgcagaa gcttgcggac gggaaaacgc tgtatgaaga agtgctgcgg
     7561 cgatcgcagg agtggggaaa cgcggaaaac atgatgtacg tcgtcggtgc gacgcgtgcg
     7621 gaatacctgg cggaaatacg gaagatcgtt ccggaccatt tcctgctcgt gccgggtgtc
     7681 ggcgcgcagg gaggaagtct ttccgatgtg gcgaaatacg gcatgaacaa agaatgcggg
     7741 ttgctggtga acgcttcgcg ctcggtactt tatgcttcgg acggtgaaga ttttgcagag
     7801 aaagcgagag aagaagcgat gcggatgagg gaggagatga agacgctgtt gatggataat
     7861 taaaaaatga aggtgaaatt attccgtacc cgcgcgtccc ggtggtgagt gaatatcatg
     7921 attgatcagc cacaaggttc acgaaattcc gcataaactc cgcaaagtaa aatttccgca
     7981 tctccgtggt gaaaaaacag tgcggcgcaa cagctttgcg ttttccttac attttcgaga
     8041 tcagcgttgc cagcgaaacc tcgatacgct gcggaaaacc tttcccgtgc gcttcttcgt
     8101 ccttcaggta gacgaggcac tgcgcaggat cttttttgaa gatcgagctg tatttgtcca
     8161 tccagatgac caggtgatct ttcatctcgc tgagatcttt ccggaaatca ttcaaagctg
     8221 catcgccggt tttcatcgca aacattcctg cctgcgcgtt cgcccattcg agcaggcgct
     8281 tgttcgccga gtacatggaa ttttccgaaa tgccgcggat gagatcgaac agcgtttttt
     8341 cttccggcgt cagctcgctg aaatgtttcg cgaagaactg gttgtagccg agtccgccgc
     8401 gatcgaaacg ctcttcaagc gaacggttga gccgggcacg caggtaattc tgcgaatgca
     8461 gcgtcgattt tccttcacgc agtatttcct gcagctggtg cagcttttcc agctcgttct
     8521 ctttgttttc ttttcttttg gcgaatttat cgtaggccgt tttcgctgcg aacgtaacga
     8581 acccgccgat cagcgcggtg atccagtcgg gaagagaagt gagactttgc atggttttgt
     8641 tgtttatcgt tccgggttga acagtttagg gtttgaatag tttcggattt cgggtttgga
     8701 ttgcgccgtt ctttgtgaat cgctgtgacg acttccgtgg tttttgtgtt aaaaaaaatt
     8761 tcagtttcgg tttggttgta ccctattctc tgtgaatcgc tgtgaccatc tccgtgttct
     8821 ttgtgtttaa aaaaaatatt ttcagtttcg gtttggttgt gtcctattct ctgcgaacct
     8881 ctgtgacaat ctccgtgctc tttgtgttta aaaaaaatca gtttcgggtt ttcaggtttg
     8941 ttgctgcgca attgcccatt accagttttc cacccgcttc atgtaatcat caatagcgaa
     9001 cggagttgtt tttgtcgccg cgctcattcg cagcggatgc cggggatgac catctttcgt
     9061 cactccgccg atcgtcaccc atcggcagtg gtgttttttc gcaacgtgat aaatatcgcg
     9121 caggcactgg gagaggaaat cccgtttatc tatcagcgtt ccccacgccg cccagatcac
     9181 cggccggcct tttatcccgg aaagcatttt ttcaatggcc gcgaggtttt tccggtgaat
     9241 gaccgcgtcc gctttgcgat gaagatgatc cgggtccgtc gcgcgctgcg ggtaaacgtt
     9301 gagcatgatc cagctgtcga acccgttgct tttcgccagg cgggccaccg acttcagcgt
     9361 attgtcgagg cggtccggtt ctgctgtgct cgggttgatg ccgatgcaga ccaggggacg
     9421 ctttccttcc gttcccagga tatagcggac cgtgttgtgg tgatcgttgt ggtagagcca
     9481 gggcacgagt gttttcagtt tcgggttttc tttttcaggc atgaattgtt ttcgcttcgc
     9541 tggctgctgt tatctgtgtt tctccggtgg ttaaaaagtt gtggagtttc aagcttggga
     9601 tttgaaatgt tttcagttcc ggttaacggt ttcctgttct tcgtgagttt tgagaagcaa
     9661 ctttcatgtg ctcggtcgga agaactcctt tgaagttttg aaaaaaaatc acgcaaagcc
     9721 gcaaaacaaa actgtggtca gtccgggcga aatctgcaac agtttaccat taattatcct
     9781 ggtcggcatc gttcaatcc
//
LOCUS       DASTSF010000416         5969 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1100927,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000416 DASTSF010000000
VERSION     DASTSF010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5969
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1100927"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="VFU15_12000"
     CDS             <1..214
                     /locus_tag="VFU15_12000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEU4718553.1"
                     /translation="RVQKKNSSVFELHSDNKAYEPYEVKAQDVLEVWEFVCSLNLSDK
                     KEDEINLESVMKMLRDLQVEMKAMKK"
     gene            complement(349..1491)
                     /locus_tag="VFU15_12005"
     CDS             complement(349..1491)
                     /locus_tag="VFU15_12005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718554.1"
                     /translation="MPRIVLFLSLGLLLLSGCTKRWMGVIVKKRVYMDGYYVHIPAKR
                     KTFPRDYVPPSPYPVTYPHTPDYVAYNDTAQPPVYVVPADTTPQQIPVAYNPPDTAGP
                     GGFGSAPPSTPGGGTAGQTPAPAGSNYILPPDTAHSDPSPPVTATANADTLHPSPPGS
                     PVSPDSSFAGEEYPAKKYASGAGDPGDISVFFQLGFINGPPVNGRPVKWNSFASCMGL
                     RYSRNVSASDKIVADAGYRFTRFYFAGDKMKTAPLPQQAHDREKMSLHAFTFALCNRI
                     CFAPYRNGAEKWFDVGLYNDLGFHSAYVYADQWHDAHSPDGTNFKTKTRITGLPYIRH
                     WNFGVTARCSTEFFNIFATYRLSRLTGANDFPDLPRFTVGLEITAG"
     gene            1544..1711
                     /locus_tag="VFU15_12010"
     CDS             1544..1711
                     /locus_tag="VFU15_12010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718555.1"
                     /translation="MDGYNAGGGKSNSKAHFLCIAEKAIGTVEKKWEKKPGEKIYRPL
                     WELLNTLNGTG"
     gene            complement(1718..2278)
                     /locus_tag="VFU15_12015"
     CDS             complement(1718..2278)
                     /locus_tag="VFU15_12015"
                     /inference="COORDINATES: protein motif:HMM:NF041635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STM3941 family protein"
                     /protein_id="HEU4718556.1"
                     /translation="MTTREFPRKKGQIAFSVVLSIAVIISVSWFIRNKMKEGVDFGTT
                     WLFFAGFLDFVFVVFLIQSLRRLFAKGPGIIISDEGIMDNTGFLAGRLLRWENAVKCD
                     NVRNGIIEMVAVKVLRPEELTMGLGSTARRAMEKNIVKYGTPFVLSPSLAEFSVIEMY
                     KLVEEKIAQKGAGSGAQQAAGPVPAE"
     gene            2365..2913
                     /locus_tag="VFU15_12020"
     CDS             2365..2913
                     /locus_tag="VFU15_12020"
                     /inference="COORDINATES: protein motif:HMM:NF014618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HEU4718557.1"
                     /translation="MKRFIYPVTALTILLGATFFFPLKGAPRSAVIVTDTAATSFPAF
                     RDTFEVKIGETETYYGDSYIINLEPKKKKGRYVLPVVIGSWEFAAINQQLNEMPPPRP
                     MAYDLMTSLVSKTGMNVRSVVIIKLEDNVFYALVLIDNKGQKLAIDSRPSDAINIALR
                     SKAPIYVERDVFDAAKEEKTKK"
     gene            complement(3011..4048)
                     /locus_tag="VFU15_12025"
     CDS             complement(3011..4048)
                     /locus_tag="VFU15_12025"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HEU4718558.1"
                     /translation="MKKLIAFIAASSFGVLLNAQDVHFSQMTFTPLLLNPAQTALGHD
                     FQAIVNYKDQWKIAALQPYRTFNTSADVAFLKKTNGNHLGLGLDVFSDKAGEGNMGTT
                     TGQLSLSGVLAADNNNFISGGLYSGFGQRTLQPDKLHWDNQYDGMQYDATLPSGEPGT
                     FANRTYFDIGAGIGWFYSKGHSTLSSNDQKKFTAGFSVQHLNRPAYSFYGDNTERLPM
                     KFVAHGNAEIGIKNYSLVLEPAYIVMIQAGHHEINAGMMVKYITRESSKYTGRKSASG
                     IALGGYYRFGDAIALAARYEFSNWSIGTSYDVNISDLKTASKARGGLEISLRFVTPDP
                     FGKTSTSKLFD"
     gene            complement(4067..>5969)
                     /locus_tag="VFU15_12030"
     CDS             complement(4067..>5969)
                     /locus_tag="VFU15_12030"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HEU4718559.1"
                     /translation="GCTTTNSFSITQPSALSATATSVNATCNSACNGSATATGSGGTG
                     PYTYSWSNSQSGATATALCAGSYTVTITDNNGCTTTQTATITEPVALGGTGSATPATC
                     GNNDGTATVSPSGGTGPYSYSWAPSGGTAATASNLGAGVYTVTITDANACVTTVSVSV
                     PSSGGPTTSVQSTGDVSCFGGSDGSGTISASGNGPFSYSWSPSGGNAVSATNLQAGTY
                     LVTVTDAGGCTSIETVTITQPAQITSTVTAVPSTCGNSDGSASVAVSGGTGSFTYAWS
                     NAQTGDTLTNVLAGTYSVTITDGNGCTSTATAVITNSGAPNITVQSSGNVTCNGGNNG
                     TATISATGGTPPYTYAWTPSGGNAATANNLAAGTYTVTVTGSDGCSQAQTVTVTEPPA
                     IQLTGTSVPENCGATDGSATVSASGGTPGYTYAWNTGGNAATISNLSAGSYTATVTDA
                     GGCSQTTTVVVGGTGSATADAGTGTTITLGQSAQLNGSGNGTFVWTPTGSLSCSSCQN
                     PIATPTVTTTYVLTVTDSLGCTAQDTVTIFVDVECGTVYVPNAFSPNGDHQNDVLFVR
                     GNCIKYMDFSVYNRWGEKVFYSTDPSVGWDGTWRDKPCEAAVFTYVLKATLMDGTEVE
                     DQGNISLVK"
BASE COUNT         1313 a         1613 c         1660 g         1383 t
ORIGIN      
        1 gcgcgtgcag aaaaaaaact cttccgtgtt cgagcttcat tccgacaaca aagcgtacga
       61 accgtatgag gtgaaagcgc aggatgtgct cgaagtttgg gaattcgtct gcagcctcaa
      121 cttgtccgat aaaaaagaag acgagatcaa cctcgaaagc gtaatgaaaa tgctccgcga
      181 cctgcaggtg gaaatgaagg cgatgaaaaa ataagcgggt tcacgatccg ggtttcatcc
      241 gcgtccgcgc gggaactctt tttatttgaa cacagcgtgc acgaatatct ttcgcaaagc
      301 acgcaaaggt tttccggaac tgaaaactga aaaaacagta acgtttcctc acccggcagt
      361 aatttccagt ccgacggtaa accgcggcag gtcgggaaaa tcattcgcac cggtcagccg
      421 gctcaggcga tacgtggcga aaatgttgaa gaattccgtg ctgcagcgcg cggtcactcc
      481 gaaattccag tggcggatgt acggcaatcc cgtgatgcgc gttttggttt tgaaattcgt
      541 tccgtccggg gaatgcgcat cgtgccattg atcggcatac acgtacgcgg aatgaaaccc
      601 gagatcattg tacaacccca cgtcgaacca tttctccgcc ccattgcggt acggcgcgaa
      661 acagatgcgg ttgcacagcg cgaacgtgaa cgcgtgcagc gacatttttt ccctgtcgtg
      721 cgcctgctgc ggcagcggcg cggttttcat tttatcaccc gcgaaataaa aacgcgtaaa
      781 gcggtagccg gcatccgcga cgatcttgtc cgacgccgaa acatttctgc tgtaacgcag
      841 tcccatgcac gacgcaaagg aattccattt caccgggcgt ccgttcaccg gcggaccatt
      901 gataaatcca agctggaaaa acacgctgat gtcgcccggg tcaccggcac cggaagcata
      961 ttttttggcg ggatattctt cacctgcgaa cgatgaatcc ggggaaacgg gagaacccgg
     1021 cggggaagga tgcaacgtgt ccgcgtttgc cgttgcggta accggcggag aagggtcgct
     1081 gtgcgcggta tccgggggaa gaatgtaatt gctgccggct ggtgcgggag tttgcccggc
     1141 agtgcctccg cccggagttg acggcggagc actaccgaag ccgcccggcc ccgccgtatc
     1201 cggcgggtta tatgcgactg gaatttgttg tggtgtcgtg tcggcaggaa ctacataaac
     1261 aggaggctgc gcggtgtcgt tgtacgcaac gtaatccggc gtatgcgggt acgtcacggg
     1321 atatggtgaa ggcggcacat aatcgcgggg aaatgttttt cgtttcgcgg gaatgtgcac
     1381 gtaataaccg tccatgtaaa ctcttttttt gacgattacg cccatccatc ttttcgtgca
     1441 acccgaaagc agcagcagtc ccagggagag aaacaataca atacgcggca tcagggaggt
     1501 gattggtcag gtaaccgtgt tccatgcagg cagaaaatgt gctttggacg gatataacgc
     1561 gggcggagga aaaagtaaca gcaaggcgca tttcttgtgt atcgcggaaa aggcgatcgg
     1621 caccgtcgaa aagaaatggg agaaaaagcc gggagaaaaa atttaccgcc ccctgtggga
     1681 gctgctcaac acgctgaacg gcaccgggta aaaaagatca ttccgcggga acaggtccgg
     1741 ccgcctgctg cgctccgctt cctgcgcctt tctgcgcgat cttctcctcc accagcttgt
     1801 acatttcgat cacgctgaat tccgccagcg acgggctcag cacgaacggc gttccgtatt
     1861 tcacgatgtt tttttccatc gccctgcgcg ccgtgctgcc cagccccatg gtcagttctt
     1921 cgggacgaag aacttttacc gccaccattt cgatgatgcc gttgcgaacg ttatcgcatt
     1981 tcaccgcatt ttcccagcgg agaagccgcc cggcgagaaa cccggtgttg tccatgatgc
     2041 cttcatcgga aataataatg cccggacctt ttgcaaagag tcttctcagc gactggatga
     2101 gaaacacaac gaagacgaaa tcaaggaacc cggcgaagaa aagccacgtc gttccgaaat
     2161 ccacgccttc cttcatttta ttgcggataa accaggaaac ggaaatgatc accgcaatgg
     2221 aaagaacgac ggaaaacgcg atctgtcctt tcttgcgtgg gaattctctt gttgtcattt
     2281 tcggggtttt gttttggaat gagcctgtgc aaatattgcc gatcattctt taaaaagcta
     2341 attttcggta aaccaaaacc ctctatgaaa cgttttatct atcctgtaac cgcgcttact
     2401 attctcctcg gcgcaacatt ttttttcccg ctgaaaggcg ctccgcggtc tgccgtcatt
     2461 gtaacggaca ccgccgcaac ttctttcccg gctttcaggg atacgttcga agtgaagatc
     2521 ggcgaaacgg aaacttatta cggcgattcg tacatcatca acctcgaacc caaaaagaaa
     2581 aaaggcaggt atgtgctgcc cgtggtcatc ggctcctggg aattcgcagc gatcaaccag
     2641 cagctgaatg aaatgccgcc cccgcgaccg atggcctacg acctgatgac aagcctggtc
     2701 agcaaaacag gaatgaacgt aagatccgtc gtcatcatca agctcgagga taacgttttt
     2761 tacgcgctgg tgctgatcga caacaaagga caaaagctgg ccatcgactc acgcccgagc
     2821 gacgcgatca atatcgcgct gcgcagcaaa gcgccgatct acgtggaacg cgatgtgttc
     2881 gatgccgcga aggaagagaa aacgaagaag tgatgaatac cgggaacttc tctccgtgac
     2941 cggaattaaa aagccggggt cccccgcaca tgcgggttct cccggctttt agcccaaaac
     3001 aaataaacta tcaatcaaac aatttcgacg tcgaggtttt tccgaacgga tccggcgtaa
     3061 cgaaacgcaa tgatatttcc aatccgccgc gcgccttgct cgctgttttc aggtcggaaa
     3121 tattgacgtc gtagctggtg ccgatcgacc agttcgaaaa ttcgtaacgc gcagccagcg
     3181 caattgcatc gccgaaacgg taatacccgc cgagcgcaat tccggaagcg cttttccttc
     3241 ccgtgtattt cgacgattca cgcgtaatgt atttcaccat catgcccgcg tttatttcgt
     3301 ggtgcccggc ctgtatcatt acgatatacg ccggttcgag tacgagactg taattcttta
     3361 tcccgatctc cgcatttccg tgcgcgacga atttcatcgg caatcgttcg gtgttgtcgc
     3421 cgtaaaagct gtacgccggc cggttcaggt gctgcacgga aaatcccgcc gtgaattttt
     3481 tctggtcgtt cgagctgagc gtggaatgtc ccttgctgta aaaccagccg atgccggcgc
     3541 cgatatcgaa atacgtgcgg ttggcgaatg tcccgggttc accgctgggc agcgtcgcat
     3601 cgtattgcat gccgtcgtat tgattgtccc agtgaagctt gtcgggctgc aacgtgcgct
     3661 gaccgaaacc gctgtacaac ccgccggaaa taaaattatt gttgtccgcg gcaagcacac
     3721 cggaaagact gagctggcct gttgtcgttc ccatgttccc ctcgcccgct ttatcggaaa
     3781 aaacatcgag gccaagacca aggtggttcc cgttcgtttt tttcaggaaa gcgacatcgg
     3841 cgctggtgtt gaatgtcctg tacggctgca gcgccgctat cttccactgg tctttgtaat
     3901 tcacaatggc ctggaagtcg tgcccgagcg cggtttgcgc aggattgagc agcagcgggg
     3961 tgaatgtcat ctgcgaaaaa tgcacgtcct gcgcgttcag caaaacaccg aacgaagagg
     4021 cagcaatgaa ggcgatcagc tttttcatct ttctgttgtt ttgcgtttac ttgactaatg
     4081 aaatatttcc ctggtcttcc acttctgttc cgtccatcag tgtcgctttc agcacatagg
     4141 tgaacaccgc cgcttcacag ggtttgtcgc gccaggtccc gtcccaccca accgacggat
     4201 ctgtgctgta gaatactttt tctccccatc ggttgtaaac ggagaaatcc atgtacttga
     4261 tgcagttgcc gcgtacgaag aggacatcgt tctggtgatc gccgttcggc gagaatgcgt
     4321 tcggcacata cacggtgccg cactcgacgt ccacgaatat cgtcacggtg tcctgcgcgg
     4381 tacagccaag cgaatcggta acggtaagca catacgtggt ggtaaccgtg ggcgttgcaa
     4441 tgggattctg gcaggaggaa cagctcagcg aacctgtcgg tgtccaaacg aatgttccgt
     4501 tcccgcttcc gttcagctgc gcggattgtc ccaatgtgat cgtcgtccct gttccggcat
     4561 cggcggttgc agagccggtg ccgcccacca caacggttgt cgtctgcgaa catccgccgg
     4621 catcggttac cgttgccgtg tagcttccgg cagaaaggtt ggagatcgtt gccgcatttc
     4681 cgcccgtgtt ccatgcgtat gtgtagcctg gtgttccgcc agatgcggaa acagtggcgc
     4741 tgccgtccgt ggctccgcag ttttcaggaa cggatgttcc ggtgagttgt atcgcgggcg
     4801 gctcggtgac ggtcacggtt tgcgcctgtg aacacccgtc gcttcccgta acggtaacgg
     4861 tgtatgttcc cgccgcaagg ttatttgccg tggctgcatt tcctcccgac ggcgtccatg
     4921 catacgtata cggcggtgtt ccgcccgttg cgctgatcgt cgctgttccg ttgttgccgc
     4981 cgttgcaggt gacatttcct gacgactgta cggtaatgtt cggtgcgcct gaattcgtga
     5041 tcacagctgt cgcggtggaa gtgcatccat tgccgtcggt aatggtaacg gaataagttc
     5101 ctgccagcac gttggtcagc gtatcgccgg tctgtgcgtt gctccatgcg taggtaaagc
     5161 tcccggttcc gccggataca gcaacgctcg cgctgccatc gctgttgccg caggtggatg
     5221 gaacagctgt aacggtggaa gtgatctgcg ccggttgcgt gatggtaacg gtttcaatgc
     5281 tcgtgcatcc gccggcatcg gttaccgtca cgagataagt tcctgcctgg agattggttg
     5341 cagaaactgc gtttcctccc gagggactcc acgaatacga gaagggtccg ttcccgcttg
     5401 cagaaattgt tccgctgccg tcgctgccgc cgaaacagga aacgtcgccg gtcgactgga
     5461 cgctggtcgt tggtccgccc gaactcggaa cagaaacact gacggttgtg acgcatgcat
     5521 tcgcgtcggt gatcgttacg gtataaacgc ccgctccgag attgctggcc gtagctgctg
     5581 ttcctccgga gggagcccag ctgtaggaat aaggtcctgt cccgccggaa ggagaaacag
     5641 ttgctgtccc atcattgttg ccgcacgttg ccggtgttgc ggagccggtt ccgcccagtg
     5701 caaccggttc ggtgatcgtt gctgtttgcg tcgtggtgca gccgttgttg tcagtgatcg
     5761 ttacggtgta cgatcctgcg cataatgctg tagccgttgc cccgctttgc gaattgctcc
     5821 acgaatacgt gtaaggtcct gtgccgccgc tgccggttgc cgtggcgctg ccgttgcacg
     5881 cggaattaca ggtggcattg accgaagtgg ccgtcgccga aagtgcagaa ggctgcgtga
     5941 tgctgaatga attggtcgtc gtgcagccg
//
LOCUS       DASTSF010000417         9611 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_337092,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000417 DASTSF010000000
VERSION     DASTSF010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 9611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9611
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_337092"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..461)
                     /gene="uppS"
                     /locus_tag="VFU15_12035"
     CDS             complement(<1..461)
                     /gene="uppS"
                     /locus_tag="VFU15_12035"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018479947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HEU4718560.1"
                     /translation="MSWKEKVDKGNLPLHVAVIMDGNGRWAQQQGQVRVFGHQSGVNA
                     VRETAEAAAEIGVKYVTLYAFSTENWNRPKEEVEALMQLLVHTIHEETKTLQENNIRL
                     ATIGDTDSLPGGCRKELEEAIAATAGNTRMTLILALSYSSRWEITEAVKKIA"
     gene            complement(481..1278)
                     /locus_tag="VFU15_12040"
     CDS             complement(481..1278)
                     /locus_tag="VFU15_12040"
                     /inference="COORDINATES: protein motif:HMM:NF039345.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6089 family protein"
                     /protein_id="HEU4718561.1"
                     /translation="MKKFLLFVSASLVLFSANAQRTDELGLFIGGSYYTGDLNPSMIM
                     NSLTRPAVGLVYRHNFNLRLSVAGSALFGSIQGIDARSSSYEQQQRNLSFRSPVYELA
                     GRAEFNFIEYKIGDDKHEFTPFMFLGAAVFNFNPKAAFGNQWVALQPLKTEGQGKPYM
                     RTQFSIPFGAGIKTNLAKRIGLVAEWGLRKTFTDYLDDVSTVYADPGTLYSNGGPLAV
                     AVADRSTSAGSNAGRQRGNPRTKDWYSFAGLTLTFQLAHKPAHCSAY"
     gene            complement(1414..2295)
                     /locus_tag="VFU15_12045"
     CDS             complement(1414..2295)
                     /locus_tag="VFU15_12045"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012781149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HEU4718562.1"
                     /translation="MKVAIYGRITENPLPSVLQGFFEKLEKSSTEILVGKGYLEYLSG
                     QFSLGKKFTSLSDFNKIRGSADVLLSLGGDGTLLETITYVRNSGIPVLGINTGRLGFL
                     AGVTVEEALDHAVERLKGKKYSLDKRTLVKLETKENLFGEVNYALNEFTVQGRETSAM
                     LTVHTTINGEFLGSYWADGLIVATPTGSTAYSLSCGGPLMVPDARSFVITPLSPHNLN
                     VRPLIVPDDSIIKLTVEGRSSRYLASLDARSLPFDVSTELTIRKEDFKFSLIRFSDQH
                     FFQTIRQKLLWGSDTRN"
     gene            complement(2427..3881)
                     /locus_tag="VFU15_12050"
     CDS             complement(2427..3881)
                     /locus_tag="VFU15_12050"
                     /inference="COORDINATES: protein motif:HMM:NF018899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HEU4718563.1"
                     /translation="MRFSAELSLGKIIFFLFTCFFSLEVAAQDSVFADSSRYYIRSVS
                     FSGNKTTKQFILERELTFHPGDTLSGPALSARIVHSRENLLNTSLFNFVTISPSCEKD
                     SSVNGVIPVSVNVTVHERWYTWPVPIFEVSEQNFNTWWRHGRNLDRASYGFFLWRYNF
                     RGRKESIALICRFGYSVQYGAQYSIPYLNRKKTFGVTLTGTYTRTHEIAVSTQNNELV
                     YFKDRAKYIRGEALGSMMFSWRPGIYIAHTLDVRYSSLWVDDTVAKVSSGYFVQGRTR
                     MDYFSLSYRFVYDHRDIKAYALHGQYAELELTKHGLGLLRNEDLNVFYVVAGVRQYAE
                     ILPRIFLGFMIRGRFLVGPDPPYYHQRALGFATYVRGYEYYVIDGQDFLLTKLNLKYQ
                     IVKPHVFHIPYLPVEKFNVFHIAVYGGIFADAGRVNDRTSLPADDNYLGNQTLFGYGA
                     GIDFVTYYDIALRVEYAFNRMGENGLFLHLGAPF"
     gene            complement(3908..4570)
                     /locus_tag="VFU15_12055"
     CDS             complement(3908..4570)
                     /locus_tag="VFU15_12055"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEU4718564.1"
                     /translation="MLASQLINDDIPPLKVEDTAEKALRWMDEFRVSHLPVINGKEFI
                     GLISDDDLYDLDDPDAPIGSHKLSLIKPVLHPQQHAYDVLKLMAELGLEVLAVTDEHG
                     TYLGSVTLPRLAEKLSRLAAVREPGGILVLEVNNVDYSLAQIAQIVEGNDAKILSCYV
                     AGIPESTKVEVTLKVNRDDLSRIVQTFNRYNYTVKATFHQSAYEEDLRKRYDEFMNYI
                     SM"
     gene            4775..5491
                     /locus_tag="VFU15_12060"
     CDS             4775..5491
                     /locus_tag="VFU15_12060"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010602343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HEU4718565.1"
                     /translation="MTRLSVNINKIATLRNSRGGNTPDLLKMAADCERFGAEGITVHP
                     RPDERHIRYADVRALKNIVTTELNIEGNPREKKFLDLVFEVKPQQVTLVPDSTNQLTS
                     DHGWDTIANRDYLKEIIAGFKRQGIRTSIFVDPNPSMVKAAAVTGTDRVELYTEAYAK
                     NFSSGKEMAIAPFIDASRMAANVGLGVNAGHDLNLHNLGYFAKNIHGLLEVSIGHALI
                     SDALYYGMENTIMMYKRLLT"
     gene            5586..6356
                     /locus_tag="VFU15_12065"
     CDS             5586..6356
                     /locus_tag="VFU15_12065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018479960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEU4718566.1"
                     /translation="MKLFFRKSGHGKPLVILHGLFGISDNWASLARMWSEHFAVYAVD
                     LRNHGQSPHAEEWNYSVMAEDVLELIADEKLNEVSLLGHSMGGKAAMRLALDYPQAVS
                     KLLVSDIGPKKYEEHNRKVVEALQRVHPEKLASRKEAEAMLVHDLRDNSTVQFLLKNL
                     SWKESGEKKHLGWRFNLDVIARKLDAVSEATDSPAPCPVQTLFIRGENSEYIRPEDEN
                     GIKRVFPDSEILTIKNAGHWVHADQPQAMYEAVMDFCG"
     gene            complement(6361..6936)
                     /locus_tag="VFU15_12070"
     CDS             complement(6361..6936)
                     /locus_tag="VFU15_12070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008505272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HEU4718567.1"
                     /translation="MKANKLLSLSEEKIVSRIHMIRGKKVILDRDLAEMYGVETKNLN
                     KAVKRNLRRFPDDFMFRLSPAEFSALRFQSGTSNSGSSSRGGLRYMPYVFTEQGVAML
                     SGVLNSRTAIDVNIRIIRAFTRMRELFLSQKELLLKIEKLERKLMEQGSAVRKQERDI
                     LLVFETLKRLISPPGPAPRKRIGYRRKSEND"
     gene            7313..7783
                     /locus_tag="VFU15_12075"
     CDS             7313..7783
                     /locus_tag="VFU15_12075"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013929247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEU4718568.1"
                     /translation="MTTAEIFTNKGTMKVAFYDNDAPGTVANFVKLAKSGYYDGLKWH
                     RVLPGFVIQGGCPNTREGATGAPGTGGPGYTIDCELTGGNQFHDKGVLSMAHRGPNTG
                     GSQFFICLSRAQTAHLDRAHTCFGKVTEGLDVLDNIRQGDSIDKIVVHENPAGQ"
     gene            7863..8864
                     /locus_tag="VFU15_12080"
     CDS             7863..8864
                     /locus_tag="VFU15_12080"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HEU4718569.1"
                     /translation="METHDFSSPPPPASPPGPQPLYNRFLQAGREGKNQWWRYIVGIV
                     LTFIAGYSLIGAVPVLLLVLRGVLKGYFTLGQLQGNSQAVENPAFLHVSSNTLLASLL
                     FIFVAAMFFLWIAVRFVHKKRFLSIINYEGNRFDYRRFFVAAGIWTVISIVAFAISYI
                     SDPGNFQLVFRPGPFTGTLLICLVMLPVQTGWEELFLRGYIMQGLGLWMKKPVWPLLI
                     TAVIFGLLHMQNEEVKANGALAMFPSYFLPGLVFGMIALLDERLELAMGLHFANNLFG
                     ILTVTSPDMSIQANAIWKVSTIGNPSDIIGGALSLFLVLGILWRMYKWNAQKLLRAY"
     gene            8920..>9611
                     /locus_tag="VFU15_12085"
     CDS             8920..>9611
                     /locus_tag="VFU15_12085"
                     /inference="COORDINATES: protein motif:HMM:NF036516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M61"
                     /protein_id="HEU4718570.1"
                     /translation="MKARKKTAVKGIFFFLMILFFSSAPAQQDSAFHFSLDLTKTHND
                     MLTVELVTPAMKTDTVIYRLPAIVPGTYKVYDFGRFTHGFKALDKNGKELFAEGIDLN
                     SWKIGNAKQLYKITYDVEDTWDTDVKGEFVFEPAGTNIEPDSNFVINNHGFFGYFDGM
                     KRDVYKISITHPDGFYGATGLSDVQRSGNTDVFTTQNYMDLVDGPIMYNRPDTTHIFV
                     GGADVLISVYSP"
BASE COUNT         2285 a         2509 c         2622 g         2195 t
ORIGIN      
        1 gcgatctttt tcaccgcttc ggtgatctcc catcgggagc tgtagctgag ggcgagaatg
       61 agcgtcatgc gcgtgtttcc ggcagtagcc gcaatagctt cttccagctc tttacggcaa
      121 ccaccgggaa gactgtccgt atcgccaatg gttgccagcc ggatgttatt ttcctgcagc
      181 gtttttgttt cctcgtggat cgtatgcacg agcaattgca tcaacgcttc cacttcttcc
      241 ttcgggcggt tccagttttc cgtcgaaaat gcgtaaagcg tcacgtattt cacaccgatc
      301 tcggccgcag cttcggccgt ttcacgcacg gcattcacac cgctctggtg accgaaaacg
      361 cgtacctgtc cctgctgctg cgcccagcgc ccgttaccat ccattatcac agcaacatgc
      421 aaaggcaggt tgcctttatc gactttttct ttccagctca tcctcgcgga agggaattga
      481 ttagtaagca gaacaatgtg caggtttatg tgcaagctgg aatgtgagcg tcaaacctgc
      541 gaatgaatac cagtctttcg tacggggatt tcctctctgg cgcccggcgt tgcttccggc
      601 gctcgtgctg cggtcggcaa cggcaacggc aagcggtccg ccattggagt acagcgttcc
      661 ggggtctgcg tatacagtgc tgacgtcatc gagataatcc gtgaatgttt tgcgaagccc
      721 ccattccgcc acgaggccga tgcgcttggc gaggtttgtt ttaatacctg cgccgaaagg
      781 aatggaaaac tgtgtgcgca tatagggctt gccctgtcct tccgttttca gcggctgcaa
      841 agctacccac tggtttccga aagcggcttt gggattgaaa ttgaaaacgg cggcgccgag
      901 gaacatgaac ggagtgaact cgtgcttgtc gtcgccgatc ttgtattcga tgaaattgaa
      961 ttccgcgcgt cccgccagct cgtaaaccgg tgaacggaaa ctgaggttgc gctgctgctg
     1021 ttcataagaa ctcgaacgtg catcaattcc ctggatgctg ccgaaaagcg cgctgcccgc
     1081 caccgaaaga cgcaaattga aattgtgacg gtaaacaagt cccaccgcag gccgcgtcag
     1141 gctgttcatg atcatggaag gattgagatc accggtgtaa taagagccac cgataaagag
     1201 tccgagttcg tccgtgcgct gggcgtttgc actgaacaat acgagcgaag ccgatacgaa
     1261 aaggagaaac tttttcattc ttcagcaaca ttttttatat acccagaacg catttcaggg
     1321 tcatttggta tatgaaatta cgaggcaaca aatatagccc tttttccagt cggggttggc
     1381 tggcctccgg aactgatatt aactggtttt caactagttc cgggtgtcgc tgccccagag
     1441 gagcttttgg cggatggtct ggaaaaaatg ctggtcgctg aagcgtataa ggctgaactt
     1501 gaaatcttcc ttgcgaatgg tcagctccgt cgacacgtcg aagggcagag agcgtgcatc
     1561 gagcgatgcg aggtaacgcg agcttcttcc ttccacggtc agcttgatga tgctgtcatc
     1621 gggaacaatg agcggacgta cgttcaggtt gtggggcgaa agcggcgtga tgacgaagct
     1681 ccgtgcatcg ggcaccatga gcggaccacc gcagctgagc gaataggcgg tagagcccgt
     1741 gggcgtggcg acgataagac cgtccgccca gtacgacccg aggaactccc cgttgatcgt
     1801 cgtgtgaacg gttagcatgg ccgacgtttc ccttccctgc acggtgaatt cattcagcgc
     1861 gtaattgact tcaccgaaaa gattttcttt tgtctcgagc ttgacgaggg ttcttttatc
     1921 gagagaatat ttttttcctt tcagccgctc caccgcgtga tcgagtgctt cctccacggt
     1981 aacgccggcc aggaaaccga gccgcccggt attaataccc agaacaggaa tacccgaatt
     2041 ccgcacgtac gtgatcgttt ccagcaatgt accgtcaccg ccgaggctga gcagaacgtc
     2101 tgccgagccg cggatcttgt tgaaatccga aagcgaagtg aattttttcc cgagggaaaa
     2161 ctggcctgaa agatattcga ggtagccttt gcccacgaga atttcggtac tgcttttttc
     2221 cagtttttca aagaacccct gcaggaccga aggcagagga ttttccgtga tgcgtccgta
     2281 gatggctact ttcattggga tcggccaccc gccttccatc cttgcaaaca gacggagaaa
     2341 agcggagatg gttgcccgaa gttataacat ttagaaatag gcgaaatgtc cttccgtcac
     2401 gcttaagcaa acgttaacgc cgattttcag aacggcgcgc cgaggtgaag gaaaagcccg
     2461 ttctcgccca tgcggttgaa ggcatattca acgcggaggg caatgtcata ataggtcacg
     2521 aaatcgatgc cggcgccgta gccgaaaagc gtctggttgc cgagataatt gtcatcggcc
     2581 ggcagcgacg tacggtcatt cacacgcccc gcgtcagcga atattccccc gtaaacggca
     2641 atgtggaaca cattgaattt ttccaccggc aggtaaggaa tatgaaatac gtgcggtttg
     2701 acgatctggt atttgagatt gagcttcgtc agcaaaaaat cctgcccgtc gatcacgtaa
     2761 tattcatacc cgcgtacgta cgtcgcgaaa ccgagcgccc gctggtgata atacggcgga
     2821 tcggggccga ccaggaatcg cccgcggatc atgaagccga gaaaaatgcg tggaagaatt
     2881 tcagcatact gccgcacgcc ggccacgaca taaaacacat tgaggtcctc attcctgaga
     2941 agaccgagtc cgtgcttcgt cagctccagc tcggcatatt gcccgtggag tgcataggct
     3001 ttgatgtcgc ggtgatcata aacaaaacga tagctcaggg aaaaataatc catgcgcgtc
     3061 cttccctgca caaaataacc ggatgacact tttgcaacgg tgtcgtccac ccacaggctg
     3121 gaatagcgga cgtccagcgt gtgcgcgatg taaatgccgg ggcgccacga gaacatcatg
     3181 ctgccgagcg cttccccgcg aatgtatttt gccctgtcct tgaaatagac cagctcgttg
     3241 ttctgcgtgg aaacagcgat ctcgtgcgtg cgcgtgtaag tgccggtgag cgtgacgcca
     3301 aatgtttttt tccggttgag ataaggaatg gaatattgcg cgccgtactg cacggaatag
     3361 ccgaagcggc agataagcgc aatggattct tttcgtccgc ggaaattata ccgccagaga
     3421 aaaaatccgt agctcgcccg gtccaggttc cttccgtggc gccaccacgt gttgaaattc
     3481 tgttccgaaa cttcgaagat cggcacaggc caggtgtacc accgttcatg aaccgtaaca
     3541 tttactgaca ccgggatgac gccgttcacc gaagaatcct tttcacagga aggcgagatc
     3601 gtgacgaaat tgaagagcga agtattcagc aggttttccc tcgaatgaac gatacgtgcg
     3661 gaaagagccg ggccggaaag cgtatcaccc gggtggaacg tgagctcgcg ctcgaggatg
     3721 aactgtttcg tggttttatt cccggaaaag gaaaccgaac ggatgtagta acgcgacgaa
     3781 tcggcgaaaa cggaatcctg cgccgcgacc tccagcgaaa aaaagcatgt gaacaggaaa
     3841 aaaatgattt ttcccaatga aagctcagca gaaaagcgca tcgggaacga aggtaaggca
     3901 agcggaatta catgctgata taattcatga attcgtcgta gcgcttgcgg aggtcttctt
     3961 cgtatgcgga ctggtggaac gtcgccttga ccgtatagtt gtagcggttg aacgtctgca
     4021 caatgcggga aaggtcgtcg cggttgacct tgagcgtcac ctccactttc gtgctttccg
     4081 ggatgccggc gacgtagcag ctgaggattt ttgcatcatt cccttccacg atctgcgcga
     4141 tctgcgccag cgaataatcg acattgttca cttcgagcac gagaattccg ccgggctcac
     4201 ggacagcggc gagacgggaa agcttttccg ccaggcgcgg cagagtgacg gaaccgagat
     4261 acgttccgtg ttcatccgta acggcaagca cttcgagccc gagttccgcc atcagcttca
     4321 gcacatcgta agcgtgctgc tgcggatgaa gaacgggctt gatcagggag agcttgtgcg
     4381 aaccgatggg cgcatccggg tcatcgagat cgtaaagatc atcgtcggaa ataagaccga
     4441 tgaactcttt cccgttgatc acgggcaaat ggctcacgcg gaattcatcc atccaccgca
     4501 acgctttttc tgcggtgtct tccactttca gcggtggaat atcgtcgttt atgagttggc
     4561 tggcgagcat ggtttcaatg attgtgcaaa gttactattt caacgggttc ctgtatttgt
     4621 ataacgcaaa aggcatgcaa aagtttaacg caggcggtaa ccggcccgta aaaaccaata
     4681 accgggatca atccggcgat ttctgatcgt ttataaaacc ggcggcggca gaaaaacttt
     4741 catgcgtaac tttacaatga aaaacgggaa ccccgtgaca cgacttagcg taaatatcaa
     4801 caagatcgcc acgctgcgca acagccgcgg aggcaatacg ccggacctgc tgaagatggc
     4861 ggcggactgt gaacgtttcg gtgcggaggg aattacggtt cacccgcggc cggatgaacg
     4921 ccatatccgg tacgccgatg tgcgtgcgct gaagaatatc gtaacgaccg aactgaacat
     4981 cgaagggaat ccgcgggaga aaaaattcct cgacctcgtt ttcgaagtga aaccgcagca
     5041 ggtaacgctg gttcccgatt ccaccaatca gctgacgtct gatcacggat gggacacgat
     5101 cgccaaccgt gattacctga aggagatcat tgccggcttt aaacgccagg gcatccgcac
     5161 gtcgatattt gtcgacccca atccttccat ggtgaaagca gccgcggtaa ccggaacgga
     5221 ccgtgtcgag ctgtatacgg aagcgtacgc aaagaatttt tcctccggga aagaaatggc
     5281 gatcgctcct tttatcgacg cttcgcgcat ggcggcaaat gtcgggctcg gcgtcaatgc
     5341 gggccacgat ctcaacctgc acaacctggg ttattttgca aaaaacatcc acggtcttct
     5401 cgaagtttcc atcgggcatg cgctgatctc ggatgcgttg tattacggaa tggaaaacac
     5461 catcatgatg tacaaacggc tgctcacctg atcttttcgg ttttcaattc ccggaaggcg
     5521 agccggttaa tccattcgct gtcccgacaa atacatctcc cctaaattca ttctcaatct
     5581 ttcccatgaa gcttttcttc cgcaaatccg gccatggaaa accgctcgtt atccttcacg
     5641 ggctgttcgg gatttcggac aactgggctt cactggcgcg gatgtggtcg gagcatttcg
     5701 cggtgtacgc tgtcgacctg cgcaaccacg ggcagtcgcc gcacgcggag gaatggaact
     5761 acagcgtgat ggcggaagat gtgctggagc tgatcgcgga tgaaaagctg aacgaggttt
     5821 cgctgctcgg ccattccatg ggtggaaaag cagcaatgcg ccttgcactc gattacccgc
     5881 aggctgtttc gaagctgctt gtttccgaca tcggtccgaa aaaatacgaa gagcacaacc
     5941 gcaaggtcgt ggaggcattg cagcgcgttc acccggaaaa gctggcttcg cgcaaagagg
     6001 cggaagcgat gcttgttcat gacctgcgcg acaacagcac ggtccagttc ctgctgaaaa
     6061 atctctcgtg gaaagagagc ggcgaaaaaa aacaccttgg ctggcgcttc aacctcgacg
     6121 tgatcgcacg gaagctcgat gcggtttccg aagccacgga ttcaccggca ccgtgccccg
     6181 tgcagacact tttcatacgc ggggaaaact cggaatacat ccggcccgag gatgaaaacg
     6241 gcatcaaacg tgtgttcccc gattctgaaa tcctcacgat aaagaacgcc ggccattggg
     6301 tgcacgccga tcagccgcag gcgatgtatg aagccgtaat ggatttctgc ggatgagctt
     6361 tcagtcgttt tcgctctttc tccggtagcc gatccgtttt cgcggagcag ggccgggcgg
     6421 cgagatgagg cgcttaagcg tttcgaaaac caacaggatg tcgcgttcct gcttgcgcac
     6481 ggcgcttccc tgctccatca gttttctttc gagcttttcg atcttcagga ggagttcttt
     6541 ctgtgaaagg aaaagttccc gcatgcgcgt aaatgcgcgg atgatgcgga tgtttacgtc
     6601 gatggcggtc ctgctgttga gcacgccgga gagcatggcg acaccctgtt ccgtaaagac
     6661 ataaggcata taacgaagtc cgccccggga agaagaaccg gagtttgagg ttccagattg
     6721 gaacctcaaa gcagagaact cagcgggaga caaacggaac atgaaatcat cggggaacct
     6781 gcgcaggtta cgcttcacag ctttgttcag gttcttcgtt tcgacgccat acatttcggc
     6841 aaggtcgcgg tcgaggatta cttttttacc gcggatcatg tggatgcggc tgacgatctt
     6901 ttcttcgcta agagataaga gtttgttcgc tttcatattt taaaagttaa aattcaacaa
     6961 ggcggcaaaa gaagaaatta tttttcaccc aagccgtttg aaaacggata aggtgttttt
     7021 gagtgaaaga aaaaggagta gattttttgc ctgtctgccg tgcaggttcc ctggaaaaag
     7081 ttgcgcccta aaacgcgccg gagccgaccg gcggaattct gcacgtggaa aagttatgtc
     7141 aacgtgttcc gcacggattt ccgggcaggg tttgacaatg tgatgcgatg aaggtacttt
     7201 cgtagccccg attgatcacg cgtgcgaagc gcggcgtgag aaagcgggaa gagagaaact
     7261 gaaatgcgcg tgtggtgcgc ttctgaaaac atccactcta attcttacca gcatgacaac
     7321 agcagaaatc ttcaccaaca aaggcacgat gaaagttgct ttttacgaca atgatgctcc
     7381 cggaacggtg gcgaacttcg tgaagctcgc gaagagcggc tattacgacg gactgaaatg
     7441 gcaccgcgtg ctgccgggat tcgtgataca gggcggttgc ccgaacacgc gcgaaggcgc
     7501 taccggcgca cccggaaccg gcggacccgg ctacacgatc gactgcgagc tgacgggcgg
     7561 gaaccagttc catgacaaag gcgttctttc gatggcgcac cgcggcccca acacgggagg
     7621 atcgcagttt tttatctgcc tcagccgcgc gcagacagcg cacctcgacc gcgcgcacac
     7681 gtgcttcgga aaagtgacgg aagggctgga cgtgctcgac aatatccgcc agggcgacag
     7741 catcgacaag atcgtggtgc atgaaaatcc cgccggtcag taataaacac cacttacacg
     7801 cacattgacg cgaacaacaa ggaagccggg aatactcccg gttttctgct attattgcgt
     7861 gtatggaaac gcatgatttc tcttcacctc ccccgcccgc ttcaccgccc ggtccgcaac
     7921 cgctttacaa ccgtttcctg caggcgggcc gcgaggggaa gaaccaatgg tggagataca
     7981 ttgtcggcat cgtgctgaca ttcatcgccg gctactcgct gatcggtgcg gtaccggtcc
     8041 tgctgctcgt gctccggggc gtcctgaagg gttacttcac gctcggccag ctgcagggca
     8101 attcgcaggc cgtggagaac ccggcctttc ttcacgtcag cagcaacacg cttctcgcct
     8161 cgctgctttt cattttcgtg gcggcgatgt tcttcctctg gatcgcggtg cgtttcgtgc
     8221 ataaaaaaag atttctctcc atcatcaatt acgaagggaa ccgctttgat taccgtcgtt
     8281 tttttgtcgc cgcgggaata tggacggtga tctccattgt tgcttttgcc atttcatata
     8341 tttccgatcc gggaaatttc cagcttgttt tccggcccgg gcctttcacc ggaaccctcc
     8401 tcatctgcct ggtcatgctc cccgtacaaa ccgggtggga agagcttttc ctgcgcggtt
     8461 acatcatgca gggcctcggt ttgtggatga aaaaaccggt gtggccgctg ctgatcaccg
     8521 ccgtgatctt cgggttgctg cacatgcaga acgaagaagt aaaagcgaac ggcgcgctgg
     8581 cgatgtttcc ctcctatttt cttccgggtc ttgttttcgg catgatcgct ttgctggacg
     8641 aaaggctgga gctggccatg ggacttcact tcgcgaataa tcttttcggg atcctgacgg
     8701 tgacgagccc tgacatgagc atacaggcga acgcgatctg gaaagtttcg acgatcggga
     8761 acccgtcgga tatcatcggc ggtgcgctgt cgctgttcct tgtgcttggc atattgtggc
     8821 gcatgtacaa atggaacgcg cagaaattac tgagggctta ctgaacgacg tggtatgtat
     8881 ttcattttcc ggaaaaaaag agcggacttc ttttgcgtca tgaaagcgcg aaaaaaaaca
     8941 gcagtgaaag gcattttctt ttttttgatg atcctgtttt tttcctccgc accggcgcag
     9001 caggacagcg cttttcattt ttcgctcgac ctgacgaaaa cgcacaacga catgctgacg
     9061 gtggagctgg tcacaccggc aatgaaaacc gacacggtga tctaccgttt gccggcgatc
     9121 gttccgggaa cgtacaaggt ttatgatttc ggacgcttca cacacggttt caaagcgctc
     9181 gacaaaaacg ggaaagagct tttcgccgag gggatcgatc tcaattcctg gaagatcggg
     9241 aacgcgaagc agctgtataa gatcacgtac gatgtggaag acacatggga taccgatgtg
     9301 aaaggcgaat tcgttttcga gccggcggga acaaacatcg aacccgattc gaacttcgtg
     9361 atcaacaacc acggcttctt cggctatttc gacggcatga agcgcgacgt ctacaaaatt
     9421 tccatcacgc accccgacgg attttacggc gccaccgggc tgagcgatgt acagcgaagc
     9481 gggaacacgg acgttttcac cacgcagaac tacatggacc tcgtcgacgg accgatcatg
     9541 tacaaccgtc ccgacacgac gcacattttt gtcggcggtg cggacgtgct catttctgtt
     9601 tattcgccgc a
//
LOCUS       DASTSF010000418         5501 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_450962,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000418 DASTSF010000000
VERSION     DASTSF010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5501
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_450962"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            137..421
                     /locus_tag="VFU15_12090"
     CDS             137..421
                     /locus_tag="VFU15_12090"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEU4718571.1"
                     /translation="MATLIVKHPVADFNQWKKVFDGMHETRLAHGFTEHQVLRDPTDA
                     NLVTIISRVRSAEDAKAYGQSPVLKEAMKNAGVIGAPHVELLNDEEIKTY"
     gene            636..1508
                     /locus_tag="VFU15_12095"
     CDS             636..1508
                     /locus_tag="VFU15_12095"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEU4718572.1"
                     /translation="MKQFCSFISACLLAFALNAQNTITCDSIIQTSTCAGGNVVIPFA
                     VTGSFPFGNVFTAQLSNGFGQFAAVPPVVGTTMFTIGGNGIIFGTIPANTNFGFLYRV
                     RIVSSNPADTSSSSPNTLIVTQVAQLNTIYANPGDSACPGDTITLFAANPATSFSWST
                     GDTTQSIQVTSSGIYSVTTTDFLTCQSTTADTVVFDPAFCTGIAENFLDKQVSVFPNP
                     ANDRLNISFVDVYSLDAVLSIRDAFGKTLLEERETLHPHSVTQLDISGFAPGIYFLMF
                     DSDGQHAVRKIVVK"
     gene            1670..2806
                     /locus_tag="VFU15_12100"
     CDS             1670..2806
                     /locus_tag="VFU15_12100"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU4718573.1"
                     /translation="MISSKYGIFDNMIEGVQVIDPRDRYVYLNAAAIAQSRTSKEELI
                     GKTMQEKYPGIGDSELYRQITTCRQQRSACSMLNHFEFPDGTEGWFDLRLEPVEEGVL
                     IMSFDISKQKQMEKELQRMNEELERRVDERTKELQDSLTREKELNELKSAFVSMASHE
                     FRSPLATILSSTSLAERYGETGQEEKRQKHLNRIKASVTHLTLTLDDFLSLNKLESGK
                     AEYHSALVDLAKYSEELLEDLRLLCRNGQHINHRHVGRSGPVKLDRNVMRNVLFNLVS
                     NAIKYSPNGEDIELTTELTDDTFIICVKDKGIGIPEEDKQKMFGKFFRASNASPIQGT
                     GLGLSIVKKYVELAGGDIGFTSRMDEGTTFVVTIPQPETAVQDS"
     gene            2846..3997
                     /locus_tag="VFU15_12105"
     CDS             2846..3997
                     /locus_tag="VFU15_12105"
                     /inference="COORDINATES: protein motif:HMM:NF019912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718574.1"
                     /translation="MKKIFALAALLLLNIPFFYAQDLPGISFNGKFSIDGNCQKGMVM
                     SAHHDNNGKNYIYAACKESGLKIYNDSGRFVREIPIGEMNNLHVMNLSQNGNYLYLAL
                     GNFFASSKTPSGFAIVDVTNPTAAFVVSRWSDNSLKGGAGAVLCDGNYLFLGAMQNGI
                     LVFDISDRKNPVKLSSFIPNISFPDPKPDPPKYNARGLALRGNYLYVCYDAGGLRILD
                     VSDKKNLKEVGRYSNPVMNGKPRAYNNVAIDSNYAYVAVDYCGMEVLDVSDPGNIKQV
                     SWLDPWDCGKGGMSWFKSPGYANEIVLDKKNRLAFISAGRTPMIVADIADPSKPRLCA
                     TYDATGKDKGTWGVSVYDNRIYLTYVCAFVPFRSTWTGVEVIEYEVKKN"
     gene            complement(4060..>5501)
                     /locus_tag="VFU15_12110"
     CDS             complement(4060..>5501)
                     /locus_tag="VFU15_12110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEU4718575.1"
                     /translation="GFLIALIYPRLATGFLPDMDEGTIVLDYHSPPGTTLDETDRMLK
                     EVEKILVQVPEVQDYSRRTGAEMGFFITEPNRGDYLIQLKKDRKRTTGEVIEDIRSRI
                     ESTQPALVIDFGQIIGDMLGDLMSSAQPIEVKIFGPNPDVLRNYAQQVSGIVADTKGT
                     ADVFDGNVIAGPEVNVVPDYEKISRFGITPDDFQFQLQTHLEGTVVGSVPENQQMVNI
                     RMIYPDSKKSGVRDISRQNIFLPGGRQRPITDLAQIRINPGVSEIRREDLQSVAVVSA
                     RLDQRDLGSTMKDIRNRIDTSVSMQKGYHITYGGAYREQQDSFRELLLIMITASILVF
                     AVIIFLFRDFKAAWLILFISVLGTGGSYLALYLTHTPLNVGSYTGLIMIIGIIGENAI
                     FTFRQFILSLKSVSVDDAITFSIATRLRPKLMTAISAIIALFPLALGIGTGAQLHQPL
                     AIAVIGGFIAALPLLLIVFPSALRMIYRK"
BASE COUNT         1331 a         1416 c         1474 g         1280 t
ORIGIN      
        1 accccggaga cgctgagcag ggtgcggaaa aaaatcacgg gccgctgatc atttcttgat
       61 atttatcaag gcatgccgct tttccgcggg tgtatgtttg ccgtgttctt aatcattcac
      121 ccaaaaaaca aaccacatgg caactcttat tgtaaagcat cccgtagcgg atttcaacca
      181 atggaaaaaa gtgttcgacg gcatgcacga aacccgtctt gcgcatggat tcaccgagca
      241 ccaggtgctg cgcgacccga ccgatgcgaa cctcgtgacc atcatcagcc gcgtgcgcag
      301 cgctgaagat gcaaaagcct acgggcaatc gccggtgctg aaagaagcaa tgaaaaatgc
      361 aggcgtgatc ggtgcgccgc atgtcgaact gctgaacgac gaagagatca aaacctattg
      421 agcggttgct cgaagaaccg gccgccgcgc cggttttttt ataccttgaa tgggaaaagc
      481 gcaaccgggt caatgaagga tgaccgcgcc ggtattttca cggtgcggga aaatggatcc
      541 gtttcccttt ccggcctgat tttacggggc gtccggcgta aaacgaagtc gtcgttttgc
      601 taaattcgtt cctgcaaaaa ttgctgcacc tttctatgaa acagttctgt tccttcattt
      661 ccgcctgcct tctcgcattt gcgctgaacg cgcagaatac gatcacgtgc gacagcatca
      721 tccagacttc cacctgcgcg ggagggaatg ttgtcattcc tttcgccgtc accgggtcgt
      781 tcccattcgg aaatgttttc accgcccagc tttcgaacgg tttcgggcaa tttgcagcag
      841 ttcctcccgt ggtcggtacg acgatgttca ccatcggagg caatggaatt attttcggca
      901 cgatcccggc gaataccaac ttcgggtttc tttaccgcgt ccgcatcgtt tcttcgaatc
      961 ctgcagatac cagcagcagc agccccaaca cactcatcgt gacacaggtg gcgcagctca
     1021 atacgatcta tgcgaatccc ggcgattcgg cttgtcccgg cgatacgatc acgcttttcg
     1081 cggcgaatcc cgccacttca ttttcctggt cgaccggcga tacgacgcaa tccattcagg
     1141 ttacatcttc cggtatttat tccgtaacga ccaccgattt cctcacctgc cagagcacga
     1201 cggcggatac ggttgttttc gatcccgctt tttgcaccgg gattgcagaa aattttctcg
     1261 ataagcaggt ttctgttttt ccgaatcccg cgaatgaccg cctgaatatt tccttcgttg
     1321 atgtttattc gctggatgcc gtgctgtcga tcagggatgc tttcggaaaa accctcctcg
     1381 aggaaagaga aaccctgcac ccgcattcgg tgacacagct cgatatttca ggattcgctc
     1441 ccggtattta tttccttatg ttcgattccg acggacagca tgccgtgcgg aaaattgtcg
     1501 tcaagtaaag atattctcag ggttctgcac gcatttaccg ggttgttgta aaaggcaaat
     1561 cggtttagcc ggtagtaaca tatttattag cgaagtttaa atttttagtt ataaaacata
     1621 ttctaaattt gaaataatac agccaaccgg caaagcctga ccagctttta tgatctcaag
     1681 caaatacggc attttcgata acatgatcga gggcgtccag gtgatcgacc cccgcgaccg
     1741 gtatgtttac ctcaatgcgg cggccattgc gcagagcagg acttcgaaag aggaattgat
     1801 cgggaaaacg atgcaggaaa aatatcccgg catcggcgac agcgagcttt accgccagat
     1861 caccacctgc aggcagcagc gcagcgcctg cagtatgctc aatcattttg aattcccgga
     1921 tggcacggaa ggctggttcg atcttcgcct cgaaccggtg gaggagggcg tgcttatcat
     1981 gtcgttcgat atttcgaaac agaagcagat ggaaaaagag ctgcagcgca tgaacgaaga
     2041 gctggagcga agagtggatg aacggacgaa ggagctgcag gattcgctta cacgcgaaaa
     2101 agagctgaac gagctgaaat cggcgttcgt ttccatggcg tcgcatgaat tccgttcccc
     2161 gctggccacc atactttcgt ccacttcgct tgcggaacgc tatggggaaa ccggccagga
     2221 agaaaaaaga cagaagcacc tcaaccggat aaaagcgtcc gttacacacc ttacgcttac
     2281 gctcgacgat ttcctttcgc tcaacaagct ggaatccggg aaagcggaat atcattccgc
     2341 gctcgtcgat cttgcaaaat attccgaaga gctgctggaa gaccttcgcc tcctgtgcag
     2401 gaacgggcag cacatcaacc accggcatgt cgggcgcagc ggtcctgtga agctcgaccg
     2461 caacgtcatg cgcaacgtgc tgttcaatct cgtctcgaac gcgataaaat attcgcccaa
     2521 cggcgaggac atcgagctga cgacagagct gaccgacgat acgttcatca tctgcgtgaa
     2581 agacaaaggc atcggcattc ccgaggaaga caagcagaaa atgttcggca aatttttccg
     2641 cgccagtaat gcctcaccca tccagggaac cgggctcggc ctcagcatcg tgaaaaaata
     2701 cgtggagctg gccggcggcg atatcggttt caccagccgg atggacgaag gcacgacctt
     2761 tgtggtgacc attcctcaac cggaaacggc ggtgcaggat tcctgacctt tccgtacccg
     2821 gattgttttt tttaacctcg acagaatgaa aaagattttt gcattggcgg cattgctgct
     2881 gttgaacatc ccgttttttt acgcgcagga ccttcccggg atttctttca acgggaaatt
     2941 ttccattgac ggcaattgcc agaaaggaat ggtcatgtcg gcgcaccacg acaataacgg
     3001 gaagaactat atctacgccg cgtgcaagga atcggggctg aaaatttaca atgattcggg
     3061 tcgcttcgta cgggaaattc ctatcggaga aatgaacaat cttcacgtaa tgaacttgtc
     3121 gcagaacggg aattacctgt atctcgcgct cggaaatttt ttcgccagct cgaagacgcc
     3181 ttctggtttt gccatcgtgg atgtgacaaa tcccacggct gcatttgtcg taagcaggtg
     3241 gtcggacaat tctctgaaag gcggagcagg agctgtcctt tgcgatggaa attatctctt
     3301 tctcggcgcc atgcaaaacg ggatcctggt tttcgacata agcgaccgga agaacccggt
     3361 gaagctttcc tctttcattc ccaatatcag cttcccggat cccaaaccgg atccgccgaa
     3421 atacaacgca cgcggacttg cgctgcgcgg aaattatctc tacgtctgtt atgatgcggg
     3481 cggtttgcgc attcttgatg tatcggacaa aaagaacctg aaggaagtgg ggcgttattc
     3541 caatcccgtg atgaacggga aaccgcgtgc atacaacaac gtcgcgatcg acagcaatta
     3601 cgcgtacgtg gccgtcgatt actgcggcat ggaagtgctc gatgtttcgg atccggggaa
     3661 tataaagcag gtgagctggc tcgatccgtg ggattgcgga aaaggcggaa tgagctggtt
     3721 caaaagccct ggctacgcga acgagatcgt tcttgacaaa aagaacaggc ttgcatttat
     3781 ttccgcggga cgaacgccga tgatcgtggc ggacattgcc gatccgtcga aacccaggct
     3841 ctgcgcaaca tacgacgcaa ccggcaagga caaaggaacc tggggcgttt ccgtttatga
     3901 taacaggatt tatctcacct acgtgtgcgc gtttgttcct ttccgttcaa cctggacggg
     3961 tgtggaagtg atcgaatatg aagtgaagaa aaactgaagt cggaatttca aatcccgaat
     4021 gataaaatcc cggttgggga attttgagat acgttcggtt cacttcctgt aaatcattct
     4081 tagcgccgaa ggaaaaacga tcagcagcag cggaagcgcg gcgatgaatc ccccgatgac
     4141 agcgatcgcg agcggctggt gaagctgtgc gcccgttccg atgccgagcg caagggggaa
     4201 cagggcgatg atcgcgctga tggcggtcat cagtttcgga cgaagacgcg tggcgatgga
     4261 aaaagtgatc gcatcgtcga cgctgaccga tttgagcgaa aggatgaact gccggaacgt
     4321 gaagatcgca ttctcgccga tgatgccgat gatcatgatc agcccggtat agctgccgac
     4381 gttcagcggt gtgtgtgtaa ggtaaagcgc gagatagctt ccgccggtcc cgagaacgga
     4441 aatgaaaagg atcagccatg cagctttgaa atcgcggaaa agaaaaatga tgacggcgaa
     4501 aacgagaatg ctcgccgtga tcatgatgag cagcaattcc cggaaagaat cctgctgctc
     4561 acggtaagcg ccgccgtacg tgatgtgata gcctttctgc atggagaccg aagtgtcgat
     4621 ccggttgcgg atatctttca tcgtcgagcc gaggtcgcgc tggtcgagcc gcgcggaaac
     4681 aactgccacc gactgaagat cttctctccg tatttcggaa acgccgggat tgatcctgat
     4741 ctgcgcgaga tcggtgatgg ggcgttgcct tccgccgggc aggaaaatgt tctgccggga
     4801 aatatcgcgc acgcctgatt ttttgctgtc gggatagatc atccggatgt tcaccatctg
     4861 ctggttttcc ggaacggaac cgaccaccgt tccttccagg tgtgtctgca gctggaactg
     4921 gaaatcatcg ggcgtgatcc cgaagcgcga aattttttca taatccggaa cgacgttcac
     4981 ttcaggtccc gcgatcacgt tgccgtcgaa aacatcggct gttcctttcg tgtccgccac
     5041 gatgccggaa acctgctgcg cataattgcg gagcacgtcg ggattgggtc cgaatatttt
     5101 cacttcgatc ggctgtgcgg agctcatcag gtcgccgagc atgtcgccga tgatctgccc
     5161 gaaatcgatg accagcgcgg gctgcgtgga ctcgatgcgt gagcgtatgt cttcgatgac
     5221 ttcacccgtt gtccgtttac ggtctttttt cagttgaatg aggtaatcgc cgcggttggg
     5281 ttcggtgatg aagaatccca tttccgctcc tgtacgccgc gagtagtcct gcacttcggg
     5341 aacctgcacg agaatttttt ccacttcctt cagcatgcgg tcggtttcat cgagcgttgt
     5401 tcccggcgga gaatgataat cgagcacgat cgttccttcg tccatgtcgg gaaggaatcc
     5461 cgtggcgagc ctggggtaaa tgagcgcgat gagaaaaccg a
//
LOCUS       DASTSF010000419         2337 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_867025,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000419 DASTSF010000000
VERSION     DASTSF010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2337
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_867025"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..848
                     /locus_tag="VFU15_12115"
     CDS             <1..848
                     /locus_tag="VFU15_12115"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEU4718576.1"
                     /translation="GSNRPGEWFWSLGFASVDSTDGVLMSNSWTNMPSTPVDNILVTP
                     RLQIVDGNAQLSWRSAVRQTPHYLDGYMVLVSTSNNDPSSFTDTIFRASEYVSQDNAA
                     FPYDFSSYTFDPAPTANPLSPFVHGMDGTYTVNEGNDSERLVGVLRPFTVSLAAYSGM
                     TIYVEFDHYSHDDNLLCIDDILVTGTNPSSVAENNSGVTVGAYPNPANDQLTVNLGLK
                     AATNVTVAMYDLSGQLVSTQDFGMQQEGQHRLDMNTAGLAAGMYIMEVQTSTGKTTAR
                     VAIQH"
     gene            1055..1846
                     /locus_tag="VFU15_12120"
     CDS             1055..1846
                     /locus_tag="VFU15_12120"
                     /inference="COORDINATES: protein motif:HMM:NF012571.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine-4-hydroxylase"
                     /protein_id="HEU4718577.1"
                     /translation="MQQHWNKYTNEDHAVWKILFERQVKNLADKAWSRYLTCIPLSGQ
                     HADAVPDFSIVEKHLARTTGWTIEVVKGIIPVGEFLSLLSRKKFCSSTWLRQRHQLDY
                     LEEPDMFHDTFGHIPLLADEDYSAFVKNFADMGLKFCHNEKAVMLLERLYWFTIEFGL
                     MKENDALKIYGAGILSSFGESNHVFSANVVLKDFSVRDVLLQPFHNNEVQNIYFVVNG
                     MNELWNCIPETEKFLEDFVDGKFPEEDFQFEKQLGSAMAEMMSDE"
     gene            complement(1953..>2337)
                     /locus_tag="VFU15_12125"
     CDS             complement(1953..>2337)
                     /locus_tag="VFU15_12125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007081511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU4718578.1"
                     /translation="WVLRFGLFAFGDPSGGLWMIILSCIVYGMAFDFFNISGSLFVET
                     SASPDIRSSAQGLFMMMTNGCGAVLGSSLSGYIIQRFFAAADGTWDWRGIWLTFAIYA
                     FVVAVLFSLFFRHRHDPEAIGEVKH"
BASE COUNT          601 a          652 c          601 g          483 t
ORIGIN      
        1 gcggctccaa ccgccccggc gaatggttct ggtcgctcgg tttcgccagc gtcgattcca
       61 ccgatggcgt gctcatgagt aattcctgga cgaacatgcc ctcgacgccg gtcgacaaca
      121 ttctcgtgac gccccgcctg cagatcgtcg acgggaacgc gcagctttcc tggcgttccg
      181 ccgtgcgcca gacaccgcac tacctcgacg ggtacatggt cctcgtttcc acctcgaaca
      241 acgatccgtc gtctttcacc gacacgattt tccgcgccag cgaatacgtt tcgcaggaca
      301 atgcggcttt cccttacgat ttctcttcct acacattcga tcccgctccc acggccaacc
      361 cgctcagccc gttcgtacac ggcatggacg gaacatacac cgtgaatgaa gggaacgatt
      421 ccgaaagact cgtcggtgtg ctgcgcccgt tcaccgtttc gctggctgct tattcaggaa
      481 tgacgatcta cgtggaattc gatcattatt cgcacgacga caacctgctc tgcatcgacg
      541 acattctcgt gaccgggacg aatccttctt ccgtggcgga aaacaattcc ggcgttacgg
      601 tcggcgctta cccgaatcca gcgaatgacc agctcaccgt gaatctggga ctgaaagcgg
      661 cgacaaacgt cacggtagcg atgtacgacc tttcggggca gcttgtgtcc acacaggatt
      721 tcggcatgca gcaggaaggc cagcatcgtc tcgacatgaa tacagcggga cttgcagcgg
      781 gaatgtacat catggaagtg cagacgtcga ccggaaaaac aacggcgcgc gtcgccatcc
      841 agcattgatt tgctacattt gacgccatga aactgttcaa cggaaattat tattacgatc
      901 accggtcccc cgcggagctc ggttgacgtg atatttccca aacaatgtca tcgcccggtc
      961 cgcaaagacc gggcgatttt ttttaccggc gccgggtgtc cgactgcgaa cgtacgggag
     1021 ccggatcaca aacatcattt caaaacaaac gatcatgcag caacactgga acaagtacac
     1081 gaatgaagat cacgcggtct ggaagatcct gttcgaacgg caggtgaaaa acctcgcgga
     1141 caaagcgtgg agccgctacc tcacatgcat cccgctttca ggacaacacg cagatgcagt
     1201 tcccgatttt tcgatcgtgg aaaaacacct tgcaagaacc accggctgga cgatcgaagt
     1261 ggtgaaaggg attatcccgg tgggtgaatt cctttcgctt ttgtcgcgga aaaaattctg
     1321 ctcgtccacg tggctgcgcc agcgtcacca gctcgattat cttgaagagc cggacatgtt
     1381 ccatgatacg ttcggtcaca tcccgctgct ggcggatgaa gattattccg cgttcgtgaa
     1441 aaatttcgct gacatgggac tgaaattctg ccacaacgaa aaagcggtaa tgctgctcga
     1501 acgtttgtac tggttcacga tcgagttcgg actgatgaag gaaaatgacg cgttgaaaat
     1561 ttacggtgcg ggaatccttt cttctttcgg ggaaagcaat catgtcttca gcgctaacgt
     1621 cgtgctgaag gatttttccg tgcgtgacgt attgctgcag cccttccaca acaacgaagt
     1681 gcagaacatt tatttcgtgg tgaacggcat gaatgagctc tggaattgca ttcccgaaac
     1741 ggaaaaattc ctggaagatt ttgtcgatgg gaaattcccc gaagaagatt ttcagtttga
     1801 aaagcagctg ggcagtgcga tggcggaaat gatgagtgat gaataataaa tttcagaaaa
     1861 aacgctgcgc agctacaggc agaaaatgag caccagggaa aacgccggaa gagcgcgaac
     1921 acaggaagga ggcgaaaatt tcccgggacg catcaatgct tcacttcccc gatcgcttcg
     1981 ggatcatgcc tgtgccggaa aaacagcgag aacaacaccg caacaacaaa tgcgtagatc
     2041 gcaaacgtga gccagatgcc gcgccagtcc cacgttccgt cggcagcggc gaaaaagcgc
     2101 tggatgatgt aaccgctcag cgaacttccc agcacggcgc cgcatccgtt cgtcatcatc
     2161 atgaataacc cctgcgcact ggaacgtata tccgggctcg cggaagtttc cacgaaaagc
     2221 gacccggaaa tattgaagaa atcgaacgcc atgccgtaaa cgatgcacga caggatgatc
     2281 atccacaacc cacccgacgg atccccgaaa gcgaacaagc cgaaacgcag cacccac
//
LOCUS       DASTSF010000420        12701 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_695015,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000420 DASTSF010000000
VERSION     DASTSF010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 12701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12701
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_695015"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1499
                     /locus_tag="VFU15_12130"
     CDS             <1..1499
                     /locus_tag="VFU15_12130"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU4718579.1"
                     /translation="QIRPALDNYEQSLKLREKYNDTSGVAQCLSNMGVAYTIIGQKDS
                     ALSYFRKSLAIRQSIKDSAGMAYSLNNIGAIYKSENRMEEARANYAQCLAIYRSLNIK
                     VGIANTLHNIGSTYGKEQPDTALRYFRESFMLYKAIHDKANIANSCNDLAIVHLALGQ
                     KDSALKYVTQALDTALSGGYAEPIRNAYYVMTSVDSAMGNYQAAFSSYKNFVLFRDSI
                     ANEKSRKSAFEKQTQYAYEKKEALLKSKAEAKLEQQKLINWFVGSSLVLALIFSLLLF
                     NRYRLKQKNIYQQQLNEQQKQQAVAVMETQEQERKRIAEDLHDSLGHLLSTVKLHLQT
                     FPDAQKPVAEQSLLLLNQASEEIQNITFNLMPRTLEEGGLVPALNELANKVTRAGRVK
                     VLLHVHNMERFVLEKQSQFNIYRIVQEAVNNILKHAEASEINIQLVGQNDHISIMIED
                     DGKGFDPGEKKSGRGLKNIVTRSLWLKGSINIDSSPGRGTTITTEIPV"
     gene            1503..2144
                     /locus_tag="VFU15_12135"
     CDS             1503..2144
                     /locus_tag="VFU15_12135"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU4718580.1"
                     /translation="MAIRILIADDHRVFIDGMKALLNDVPGIEVIADAENGQELIEQA
                     IAHAPDVVLTDIQMPVKDGIEATKEILRISPSVKIIALTMLNESLFIRRMLDAGAYGY
                     ILKTVGKEELIGAIRKVAAGEKYFSSEAATQLLNDFSGKSSGKNNPADTLTKREKEIL
                     ALIAQGFTDKEIAEKVFLSPLTIITHRKNILSKLGLKNKVELTRFAMEHGLIG"
     gene            2261..2893
                     /locus_tag="VFU15_12140"
     CDS             2261..2893
                     /locus_tag="VFU15_12140"
                     /inference="COORDINATES: protein motif:HMM:NF027718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5011 domain-containing protein"
                     /protein_id="HEU4718581.1"
                     /translation="MKTRMIFCSAFLSAALFFSGCRKENMPPVVTLNGDANMTSYKGD
                     TFTDPGATANDNEEGDLTAKIQETGSVGSGVGYYTLDYSVTDKEGSYADVQRNVTVKY
                     KNAYLAGNYNVTETSPFGTTNYTGMVTSGTSDNAEFVFGSTSGPDPIVVDANITGLND
                     ISPLTVAQGGPLTNFTGTITEPSGHVVFTMSYLRPIGNTTTNCTATWTKQ"
     gene            complement(3057..6542)
                     /locus_tag="VFU15_12145"
     CDS             complement(3057..6542)
                     /locus_tag="VFU15_12145"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG-like jellyroll fold domain-containing
                     protein"
                     /protein_id="HEU4718582.1"
                     /translation="MKEAKKLLPAIFLLAFGFSANAQISFTTGLKSWFPMNGDFYNVT
                     GNCQYHPDLTTATFTSDPDGIAGDAATFTGSTRLRYLNGLVDADMANFSIDFWFSSTS
                     SSTEPLYSEQVSGGQGVWIRMEPGSNRIHAYLGNSTTFNNVYTTGAYNDGNWHHVVLT
                     AQSGNTAKLYVDGTLNGSSVQAWNTTATNTRTKIGTDDALTMYLNGNMDNVRVWNRPL
                     TSAEVTDLYNAPRISITSVSSATLCAGSSATLSFTVSGGSGAFQGGNVFYAQISDPQG
                     SFIYPLNIGSAPGTGSGTMTINIPDSVATGSNYHVRIVASDYPVASQNVQLITVNNAN
                     APTGFDLFSDLAIHYPFHGNATDVSGNNINGTIVSGITSSTGHNGNPNTALSFNGSNS
                     QLTIGKTWPINSLDATAKPFTISFWMRQSSTPGTWGYIFSCWDPTPATGLWIGTTSGG
                     KIRFRINGNNYVEGTVSNNNWIQVTAVYSGSNIKLYLNGTQQGGTVTTSGNISIAQNA
                     VMGWEPSTGGYYFTGRLEDFRLYDRALGLQEAQTLYHDGSLAGSNSPLCNGGLLQFDG
                     PTYAGLTYSWSGPSGFSDNVEDPFIANFNPYTETGTYSLTLTLNGCTGSPNTTLVQST
                     VPPVPSVTSQSFCQGNSVTLSVNGATGGQQYIWYDDPAGTSQIAGNVSSYTYAPATTD
                     TVYVGIYDGTSCYSALSPVVATMNPLPNVTASSSPANGEVCAGNSVTLNGAGAISYTW
                     TGGITDNVSFVPASSGTYTVTGTDANGCSNSATANVTVDPLPSVNFVSSPVNGIVCAG
                     SPVTLTAGGTATSYTWSGGITDNVAFTPASSATYTLTGTDANGCTNTASVSITVNALP
                     NVSVISSPANGMVCAGGQATLSGSGAASYSWSGGITDGVAFTPVSTASYSVTGTDANG
                     CSAAASVTVTVNALPVVGATSSPANGEVCAGNPVTLNGTGAASYSWTGGITDNVSFVP
                     VASGTYTVTGTDINGCSNTATANVTVDPLPSVLFSLNPDLICITAAPVSLSATPAGGI
                     FSGPGITGSSFDPASAGAGSFVLNYSFTDVNGCTGSDTAAVIVDVCTGIATPQNDNEL
                     SVVPNPSSGIFRLNAQTGSVYSIYSSTGQLVMSGKTESAATPIDLSAYASGIYYLEVI
                     LPGAKNSSGIRLVKQ"
     gene            complement(6657..9218)
                     /locus_tag="VFU15_12150"
     CDS             complement(6657..9218)
                     /locus_tag="VFU15_12150"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU4718583.1"
                     /translation="MLLTFAGLPCATAQDDATAALLDRAHHLSASFPDSTILLADSAL
                     SLAAAQGNVKQRIRAMEEKAYALTDLGRSEEAIAIYFEALKLCRPPGDNRQIAFIYNQ
                     LGYLYLGENDYAAAKNYYTLDLAVHRQMGDEKGCGNQLLNLAGVHRLLHEYDSAVFRL
                     NELEGILTLKRDTLLEGGLYNEKGILFQTTGVPDSARVYYKEAAQAWALGHYDSQLMR
                     ALFNLADLEAGQKRTEEALSLYLESDSIARKLHLFHEQVLTGKKIGEMYAAMGNTALA
                     YRYLQDAASLGDSLKIKDDAAFASRLDREFRMEQNRRTIQDQQLRIRREETRLLFVII
                     ISVCAVFLLVIVFTWYRFRQRVRAQVEAAKATFFDNVAHEIRTPLSMILAPVKVLQKK
                     SSDPDEKKQLDLAERNITRLNELITQMLDISKIDAAHYRLNESYGDLQAYFAGIAATC
                     EAQAKEKNMKFTVNVENFPSHGFFDHDALDKIIGNLAGNAIKYTPPGGEAGIDISVTQ
                     KDAHPELSISVWDTGAGISAADKERIFDRFYRADPKRGTDARGAGIGLSLVRELVTLM
                     KGKIEVESSPGNGSVFTVTLPLRLAEKNASSSAENGETLVLLVEDDSDILAFNRSLLE
                     GETYKVITAGDGAEAAAVLEKELPDIIITDLMMPGRDGLSLLKEVRSSAATEHIPVII
                     LSAKSAAETRITGVREGAQVFLSKPFVPDELIALVRSQLAILEKQQQRYRSANEDREQ
                     TLEERFAGTDPFTQKFFALAVQHLEDSGLSVERLAELMHINRSHFQRKMKTLTGYSPS
                     ELLRIIRLETALQLLREKKGNVTEVAYRCGFTSQSYFSKCFVQRFGYAPSVAGKP"
     gene            9548..11629
                     /locus_tag="VFU15_12155"
     CDS             9548..11629
                     /locus_tag="VFU15_12155"
                     /inference="COORDINATES: protein motif:HMM:NF019315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEU4718584.1"
                     /translation="MFAIIYPLAKQKGRTDKPLAQKGVLDLRSWDLQRDGNISLNGEW
                     AFSWNDLASPRPSASSSADFIKVPGIWNNHVSNGQKDGAEGFATYSLDVRLQKQHEML
                     ALNILTVSNAYDLFVNGKKIASNGTVGKDEETSKPAYAPQVVTFIPDSDCLHFVMHIS
                     NFAHCKGGFWLPVEIGTVKQIHEERDSRVLLEMFLFGCLFIMALYHFSLYLLRRSDPS
                     TLYFGLMCVIIGVRSLFTGENLVNDLYPGIDWFFARKIEFLLTFLSAPVYITFARALY
                     RREWNTTVYRIAFAFGMALCLFVLVTPARIFTLTSYVFTGYAWITSMYTIFVFIKAMK
                     RKENGAGIFLATSLFFLLTIVNDTLNQLELVHTGLYLSFGLLVVTFAQSFVISSRFAN
                     AFRTTEIYASTFRKFVPAQFLDKIAKDGIESIRAGYAQREEVAVLFSDIRSFTSLAEK
                     MSSDEVFRMLNEYLAYVEPPIRAHSGFVDKYMGDGIMALFENGDGNNSCDNAVAASLE
                     MLEQLEAFNEKRISAGEEPLRMGIGLHNGPVIIGTLGGNERMDSTAIGDAVNLTSRIE
                     GMTKMYGVPLLAGESIIRALSIRHELYCRFVDTVVAKGKNEPVAIWEISGRRSDKRLA
                     AYAKLIPAYEEAIRFFRERKIADAKKEFEKCLALFPGDTVSRIYLDRCTKFLETGVDE
                     NVSGITSLDSK"
     gene            11714..12304
                     /locus_tag="VFU15_12160"
     CDS             11714..12304
                     /locus_tag="VFU15_12160"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEU4718585.1"
                     /translation="MDYSSASRFMLKKLESELPSYCFYHDYAHTLDVLAAVETLAREE
                     SITDTETVDLMRTAAVYHDCGFFEQYRANEPIAVRIAAEILPRYGYSEPQVKFISRLI
                     LVTALDAVPADIYEQILKDADFDYLGRDDYPETALKLKEEWSVMGMNYSMHEWHGLQL
                     RFLESHVYYTSTAQRLRGPGKQKHIQKLKEILAAEK"
BASE COUNT         2947 a         3524 c         3447 g         2783 t
ORIGIN      
        1 gacagatccg cccggcgctc gacaactacg agcagagcct gaagctgcgc gagaaataca
       61 acgacacgtc cggcgtggcg cagtgcctca gcaacatggg cgtggcgtat accatcatcg
      121 ggcagaaaga ttctgcgctc agttatttcc ggaaaagcct cgctatacgc caatcgatca
      181 aagacagcgc gggcatggcg tattcgctta acaacatcgg cgcgatttac aaaagcgaaa
      241 accgcatgga agaggcgcgt gcaaattatg cgcaatgcct cgccatctac cggtcgctca
      301 atatcaaagt gggtattgcc aatacgcttc acaacatcgg gtcgacgtac ggaaaagagc
      361 agccggatac cgccctgcgt tatttccgcg aaagcttcat gctgtataag gcgatccacg
      421 ataaagccaa catcgccaat tcgtgcaacg acctcgccat tgttcacctc gccctcgggc
      481 agaaagattc cgcgctgaaa tacgtgacac aggcgctcga cacggcgctg agcgggggct
      541 acgcagaacc catacgcaac gcctattatg tgatgacgag cgtggacagc gcgatgggaa
      601 attaccaggc ggccttcagc agctacaaga atttcgtgct gttccgcgac agcatcgcca
      661 atgaaaaatc gcgcaaatcg gcgttcgaga aacagacgca gtacgcttac gagaaaaaag
      721 aagccctgct gaaatcgaaa gcggaagcga aactcgagca acagaagctg atcaactggt
      781 tcgtgggttc ctcgcttgtc ctcgcgctga tcttttccct tctcctgttc aaccgttacc
      841 ggctgaagca gaaaaacatt taccagcagc agctgaacga gcagcagaaa cagcaggccg
      901 tcgccgtgat ggaaacgcag gagcaggaac gcaagcgcat cgccgaggac ctccacgaca
      961 gcctcggtca cctgctttcc acggtgaagc tgcacctgca gactttcccg gatgcgcaga
     1021 aacccgtcgc cgaacaatcg ctgctgctgc tgaaccaggc ttcggaggaa atacagaaca
     1081 tcacgttcaa ccttatgccg cgcacactcg aagaaggagg actcgttccc gcgctgaacg
     1141 agctggcgaa taaagtcacc cgcgccggtc gtgtgaaagt gctgctgcac gtgcacaaca
     1201 tggagcgctt cgtgctggaa aaacaatcgc agttcaatat ttaccgcatt gtgcaggaag
     1261 cggtgaacaa cattctcaag cacgcggaag cgagcgagat caatatccag ctcgtggggc
     1321 agaacgatca catcagcatc atgatcgaag acgacgggaa aggattcgat ccgggcgaaa
     1381 agaaaagcgg gcgcggcctg aagaatatcg tcacgcgttc gctctggctc aaaggaagca
     1441 tcaacatcga ttcctcgccc gggcggggaa cgacgatcac cactgaaatt cccgtataaa
     1501 aaatggcaat ccgaatcctg atcgctgacg atcaccgcgt gttcatcgat ggaatgaagg
     1561 cgctgctgaa cgatgtgccc ggaatagagg tcatcgctga cgccgagaac gggcaggagc
     1621 tgatcgaaca ggcgatcgcg cacgcacccg acgttgtcct taccgacata cagatgcccg
     1681 tgaaggacgg catcgaagcg acgaaagaaa ttcttcgcat ttcgccttcg gtgaagatca
     1741 tcgcgctcac gatgctcaac gaaagcctgt tcatccgccg catgctcgac gccggcgctt
     1801 acgggtacat tttgaaaacc gtggggaaag aagaattgat cggtgcgatc cggaaagttg
     1861 cagccggtga aaaatatttc agcagcgaag cggcgacaca gctgctcaat gatttttccg
     1921 gaaaatcgtc gggcaaaaac aatcccgccg acacgctgac gaaacgggaa aaagaaattc
     1981 tcgcgctcat cgcgcaggga ttcaccgaca aggagatcgc cgaaaaagtt tttctcagtc
     2041 cgctgacgat catcacgcac cgcaagaaca tcctcagcaa gctcgggctg aagaacaaag
     2101 tcgagctcac acgctttgcg atggagcacg ggctgatcgg ctgaacgccg ctgcctgcga
     2161 cacgccttat cctccctatg gggtagaaag caaatcctct tttcgggtca tgttcccggt
     2221 tttcgtttcg cggaaatttg ctgcgtacaa atccgatact atgaaaacga ggatgatctt
     2281 ctgcagcgcc tttctttccg cggcgctttt cttcagcgga tgcaggaaag aaaacatgcc
     2341 gcccgtggtc acgctgaacg gcgatgcgaa catgacgtcg tacaaaggcg acacgttcac
     2401 cgatcccggc gctaccgcga atgacaatga agaaggagat cttacggcga aaatacagga
     2461 gaccggttcc gtgggttccg gtgtcggtta ttacacgctc gattattcgg ttacggacaa
     2521 ggaaggaagc tacgccgacg tgcagcgcaa cgtcacggtg aaatataaaa acgcttacct
     2581 cgccgggaat tataacgtca cggaaacgtc cccgttcggt acaacgaatt acaccggcat
     2641 ggtgacatcg ggaacgtccg ataacgcgga attcgttttc ggttccactt ccgggccgga
     2701 cccgatcgtg gtggatgcga acatcacggg actgaacgat atttctcctc ttaccgttgc
     2761 gcagggcgga ccgctcacga atttcaccgg cacgatcacc gagccgtcgg gtcacgttgt
     2821 tttcacgatg tcttatctcc gcccgatcgg gaacacgacg acgaactgta cagccacctg
     2881 gacaaagcag tgatggtcag gcaggagctg cgctccggga atgaatgtca gtggtcaggc
     2941 ccggaaaaaa cgtttgtttt gcagctcctg ctctttttca aaatcccgtt cactgaaaat
     3001 ggcaccgttc ccttgcgggc gcagtgccat ttccgtaaca gaaaaacttc ttcccgctat
     3061 tgcttcacca ggcgtattcc cgaactgttt tttgcgccgg gtaaaatcac ctcgaggtaa
     3121 taaattccgg atgcgtatgc ggaaaggtcg attggcgttg cagcactttc cgtttttccg
     3181 ctcatgacca gctgcccggt cgagctgtaa atgctgtaca cacttcctgt ttgtgcattc
     3241 aaccggaaaa ttcccgaaga aggattcgga acgacgctca gctcattgtc attctgcggg
     3301 gtcgcaattc ctgtgcagac atcaacgatc accgctgcag tatcgctgcc ggtgcagccg
     3361 ttcacatccg tgaatgaata attcaggacg aacgaaccgg ctcctgcgct tgccggatcg
     3421 aaactgctgc ccgtgatccc gggtccgctg aaaataccgc cggccggcgt tgcagacaat
     3481 gaaaccggcg cagccgtaat gcagatgaga tccgggttca gcgagaaaag cacggacggc
     3541 agcggatcga cggtcacgtt cgcagtcgcg gtattactgc aaccgtttat gtccgttccg
     3601 gtaaccgtat atgttccgga tgcaacgggc acaaatgaaa cgttgtccgt tattccgccc
     3661 gtccaggaat aagaagccgc acccgttccg ttcagtgtga cgggatttcc tgcgcagact
     3721 tcgccgttcg cgggagagct cgtcgcaccg acgaccggca atgcattgac ggtaaccgtt
     3781 acgctcgcag cagcgctgca cccgttcgca tccgtgccgg tgacagaata ggacgctgta
     3841 gaaacggggg tgaatgcaac accgtccgta attcctccgc tccatgaata gctggctgcg
     3901 ccgctgccgc tgagcgtggc ttgtccgccg gcacaaacca ttccgttcgc cggggaagag
     3961 atcacggaaa cattcggtaa tgcgttaacg gtgatactta cgctcgctgt atttgtacag
     4021 ccgttcgcat cggtgccggt cagtgtgtac gtcgcggaag atgcaggtgt gaatgcgaca
     4081 ttgtcggtga ttcctcccga ccaggtgtac gacgttgccg ttccgcctgc ggtaagtgta
     4141 accggagaac cggcgcagac aattccattc acgggagatg aaacgaaatt cacagaagga
     4201 agcggatcga ccgtcacgtt cgccgtcgcc gaattgctgc agccgttcgc atctgttcct
     4261 gtaaccgtat atgtcccgga tgaagcgggc acaaatgaaa cattatccgt tattccgcct
     4321 gtccaggtgt aggaaatcgc gcctgctcca ttcagtgtga cggaatttcc tgcgcacact
     4381 tcaccattcg ccggcgaaga agacgccgtc acattcggga gcgggttcat cgtcgcgaca
     4441 accggcgaca gcgcgctgta gcagcttgtg ccgtcataga tcccgacgta aaccgtgtcc
     4501 gtagtcgcag gtgcgtaagt gtaagaggaa acgttcccgg cgatctgcga tgtgcccgcc
     4561 ggatcatcgt accagatgta ttgctgtccg cccgttgcgc cattgacgga tagcgtgacg
     4621 gaatttccct ggcagaacga ttgactggtt acactgggaa cgggaggaac agtcgactgc
     4681 acgagcgtcg tattcggcga acctgtacaa ccgttgagcg taagcgtgag cgaataggtt
     4741 cctgtttccg tgtacggatt gaaattcgcg atgaacggat cttccacgtt gtcggaaaat
     4801 cctgaaggcc cggaccagct gtaggtaagt ccggcgtacg tgggtccgtc gaactgcagc
     4861 agtccgccat tgcacaacgg gctgttgctg ccggcgaggc ttccgtcgtg gtacaacgtt
     4921 tgcgcttcct gcaggccgag cgcgcggtcg tataaacgga aatcttcgag gcgcccggtg
     4981 aaatagtaac cgccggtcga cggttcccat cccatgaccg cattctgcgc aatggaaata
     5041 tttccggacg tggttaccgt tccgccctgc tgcgttccgt tcaggtacag tttgatgttg
     5101 cttccgctgt aaacggcggt aacctgtatc cagttgttgt tcgaaacagt tccttccacg
     5161 taattatttc cgttgatgcg gaaacggatc tttcccccgc tcgttgtccc gatccacaga
     5221 ccggtggcag gcgtcggatc ccagcagctg aaaatataac cccacgttcc gggcgtggat
     5281 gattgccgca tccagaagga gatcgtgaat ggtttcgcgg tggcgtcgag gctgttgatc
     5341 ggccacgtct tcccgatggt gagctgcgaa ttgctcccgt tgaagctcag cgccgtgttc
     5401 gggttgccgt tgtggcctgt tgaagaagtt atcccgctga cgatcgttcc gtttatgttg
     5461 ttgccggaaa catcggtcgc attgccgtgg aacgggtaat ggatcgcgag gtcggaaaaa
     5521 agatcgaagc cggtgggtgc gttggcgttg ttcaccgtaa tgagctgcac gttctgtgaa
     5581 gcgaccgggt aatccgatgc aacaatacgc acatgataat tgctccccgt tgcgacgctg
     5641 tcaggaatgt tgatcgtcat tgttccgctt ccggttcccg gcgcggagcc gatgttcagc
     5701 gggtaaatga aagaaccctg cggatcggag atctgcgcgt aaaaaacatt gccgccctgg
     5761 aatgcaccgg aacctccgct tacggtgaat gaaagcgttg cggaagagcc ggcacaaagc
     5821 gtagccgatg aaactgacgt aatggaaatg cgcggcgcat tgtaaagatc cgtcacctcg
     5881 gccgaagtaa gcgggcggtt ccaaacgcgc acgttgtcca tattcccgtt caggtacatg
     5941 gtcagcgcgt catcggtccc gatctttgtc cgggtattgg tggcggtcgt attccacgcc
     6001 tgcacactgg aaccgttcag cgttccgtcc acatataatt ttgctgtatt cccgctttgc
     6061 gcggtaagaa caacgtgatg ccagttgccg tcgttatacg cacctgtcgt gtaaacattg
     6121 ttgaacgtcg tcgaattccc gaggtaagcg tgaatgcggt tcgagcccgg ctccatgcgg
     6181 atccatacgc cctgtccccc gctcacctgc tccgaataaa gcggttccgt gctcgacgac
     6241 gtggagctga accagaaatc gatgctgaaa ttggccatgt cggcatccac cagtccgttc
     6301 aggtaacgca gtcgcgtgga cccggtgaag gtcgccgcat cgcccgctat tccatcggga
     6361 tcggatgtga acgtggcggt ggtgagatca ggatggtact ggcaattgcc cgtgacgtta
     6421 taaaaatccc cgttcatcgg gaaccaggat ttcaggccgg tggtaaaact gatctgtgca
     6481 tttgcggaaa atccgaaggc cagcaggaag atcgccggca acaatttttt agcttctttc
     6541 atggctgctt ttttacagtt tgatgctgca aaggtcaggc tgctttgaaa aaagtacgtg
     6601 aacacacgtt gcgaagacgt gtactcatgt tgcgtaattg gctggaagca aggatttcac
     6661 ggttttcccg cgacgctcgg ggcatagccg aagcgctgga cgaagcattt gctgaaatac
     6721 gactgcgaag tgaagccgca gcggtacgcg acctccgtca cgttgccttt cttttcgcgc
     6781 aggagctgca gggcggtttc caggcggatg atccgtagca gctcggacgg cgaatatccc
     6841 gtcagcgttt tcatcttccg ctggaaatgg ctccggttga tgtgcatcag ctccgccagg
     6901 cgttcgacgc ttagcccgga atcttcgagg tgctgcacgg caagagcaaa gaacttctgc
     6961 gtgaacggat cggttcccgc gaaacgctct tcgagcgtct gctccctgtc ttcattcgca
     7021 gaacggtagc gctgctgctg tttttccagg atcgccagct ggctgcgcac gagcgcgatg
     7081 agctcgtcgg gaacaaacgg cttggacagg aacacctgcg cgccttcacg cacacctgtg
     7141 atgcgggttt ccgccgccga cttcgccgac aggatgatca ccgggatatg ttccgtggct
     7201 gcgctgctgc gcacttcttt aagcagcgac aatccatccc tgcccggcat catcaggtcg
     7261 gtgatgataa tgtccggtaa ttctttttcc agcacggcag cagcttccgc gccgtcgccg
     7321 gccgttatga ctttgtatgt ttccccttcg agcagcgagc ggttgaacgc gagtatgtcg
     7381 ctgtcatctt caacaagcag cacgagcgtt tcgccattct ccgcggagga agatgcattc
     7441 ttttcggcta aacgcagcgg aagcgtaact gtaaatacgg aaccattccc cgggctgctt
     7501 tccacttcta tctttccttt catcagcgtc acgagctcgc gcacgagcga aagcccgatg
     7561 cccgcacccc tggcgtccgt tccccgtttc ggatcggcgc ggtaaaaacg gtcgaagatg
     7621 cgctctttat ccgcagcgga aattcctgct cccgtatccc acacgctgat ggaaagttcg
     7681 gggtgcgcgt ctttttgcgt gaccgaaata tcgatccccg cttctccgcc gggcggcgtg
     7741 tacttgatcg cattgccggc gaggttgccg atgatcttgt ccagcgcatc atgatcgaaa
     7801 aacccatgcg aaggaaaatt ctccacgttg acggtgaact tcatgttttt ttctttcgcc
     7861 tgcgcttcgc acgttgcagc gatccccgca aaatacgcct gcagatcgcc gtagctttca
     7921 ttgagcctgt aatgcgccgc gtcgatcttc gagatgtcga gcatctgcgt gatcagctcg
     7981 ttgaggcggg tgatgttgcg ctcggcgaga tcgagctgct tcttttcatc cgggtcgctg
     8041 cttttctttt gcagcacttt gaccggcgcc aggatcatcg acaggggcgt gcggatctcg
     8101 tgggcgacgt tatcgaaaaa tgtggctttc gctgcttcca cctgcgcgcg cacgcgctgc
     8161 cggaaacggt accaggtgaa cacgatcacc agcaggaaaa cggcgcatac gctgataatg
     8221 atcacgaaaa gaaggcgcgt ttcctccctg cgaatccgca gctgctggtc ctggatcgtc
     8281 cgcctgttct gctccatccg gaattcgcgg tcgaggcggg acgcgaacgc cgcgtcgtcc
     8341 tttattttca gcgaatcgcc gagggaagca gcgtcctgca ggtaacggta ggccagcgcc
     8401 gtgttcccca tcgccgcata catttccccg atctttttcc cggtcagcac ctgctcatgg
     8461 aaaagatgaa gcttccgcgc gatcgaatca ctttcgaggt aaagcgacaa cgcttcctcc
     8521 gttctcttct gcccggcttc gagatcggca aggttgaaga gggcacgcat cagctgggaa
     8581 tcgtaatgac ccagcgccca tgcctgtgcc gcttctttat aatatacgcg tgcggaatcg
     8641 ggaacgcccg tagtctggaa aagaattcct ttttcattgt acagcccgcc ttccagcagc
     8701 gtgtcgcgtt tcagggtgag aataccctcg agctcgttca gccggaaaac agcactgtcg
     8761 tattcgtgaa gaagacgatg cacgcccgcg aggttcagca gttggttacc gcagcctttt
     8821 tcatcgccca tctgccggtg cacggcaaga tcgagcgtgt aataattctt ggctgcggcg
     8881 taatcgttct cgccgagata caggtaaccg agctggttgt aaataaatgc gatctgccgg
     8941 ttgtcgccgg gcgggcggca cagcttcagc gcttcgaagt agatcgcgat cgcttcttcc
     9001 gaccggccga gatccgtcag cgcgtacgct ttttcttcca tcgcgcggat gcgctgcttc
     9061 acatttccct gcgcagcggc aagtgaaaga gcgctgtcgg caagaaggat cgtcgaatcg
     9121 ggaaatgaag cgctcaggtg atgcgccctg tcaagcaaag cggcggttgc atcgtcctgc
     9181 gctgtggcgc acggcagacc tgcgaaggtc agcagcaaaa aaaagataac gcggggattc
     9241 caaaacacgc gattcataaa cctttcatgc ctgcagcgaa tgtcatgccg tttcaaatat
     9301 aggtaaatcg ggttcccggt tccagcggcg ttccggcagg aaaaaaccat ccggaacttc
     9361 tgttttatcc cccgcagggt tcccttccgt ttcccgggtt ttgttttgaa ttatatttgg
     9421 ccacacagca tacaaaacgt tcagcacctg tttgctgccc cggtaaaacg cattcctttc
     9481 atcgcctgtg aataaaaatc cccggtcggc ccgagaattc ctgtacctcc ttgcagtact
     9541 cctgccggtg ttcgccatca tttacccgct ggcgaaacag aaaggccgca ccgacaagcc
     9601 gctggcgcag aaaggagtgc tcgatctacg cagctgggac ctgcagcgcg acgggaacat
     9661 cagcctcaac ggcgaatggg cattttcatg gaatgatctc gcttctcccc gtccatccgc
     9721 ttcctcttcc gccgacttca tcaaagttcc cggcatctgg aacaaccacg tttcgaacgg
     9781 acagaaagac ggcgcggaag gttttgccac gtattcgctc gatgtccgac tgcagaaaca
     9841 gcacgagatg ctggcgctga atatcctcac cgtgagcaac gcgtacgatc ttttcgtcaa
     9901 cgggaaaaag atcgcgtcga acggaacggt gggaaaggac gaagaaacgt cgaagcccgc
     9961 ctatgcgccg caggtcgtca ccttcattcc cgattcggac tgcctccatt tcgtgatgca
    10021 catttccaac ttcgcgcatt gcaaaggcgg attctggctt cccgtcgaga ttggcaccgt
    10081 gaaacagata cacgaagaac gcgattcacg cgtgctgctc gagatgttcc ttttcggctg
    10141 cctgttcatc atggcgctgt atcacttcag cctttacctc ctgcgacgca gcgatccgtc
    10201 gacattatac ttcggtctca tgtgcgtcat catcggtgtg cgcagcctgt tcacgggaga
    10261 aaacctggtc aatgatcttt accccggcat cgactggttc tttgcacgga agatcgaatt
    10321 cctgctcacg ttcctgagcg cacctgtgta cattacgttc gcgcgtgcac tttaccgcag
    10381 ggaatggaac accaccgttt accgcatcgc ctttgcattc ggcatggcgc tttgtctttt
    10441 cgttttggta accccggcgc gcatcttcac gctcacgtcg tacgtgttca ccggttatgc
    10501 ctggatcacc agcatgtaca cgatcttcgt cttcatcaaa gcgatgaaac ggaaagagaa
    10561 cggcgccgga atttttctcg ccacttctct tttcttcctg ctgacgatcg tgaacgacac
    10621 gctcaaccag ctcgagctcg tgcacacggg actttatctt tccttcgggc tgctcgtcgt
    10681 cacgttcgcg caatcgttcg tcatctcgtc acgttttgcg aacgcgttcc gcaccacgga
    10741 aatatacgcg agcacattcc ggaaattcgt gccggcgcag ttcctcgaca agatcgcgaa
    10801 agacggcatc gagtcgatac gcgcaggata cgcgcagcgg gaagaggtgg ccgtgctctt
    10861 cagcgatatc cgttcgttca ccagccttgc ggagaaaatg tcttccgatg aagtcttccg
    10921 catgctcaac gaatatcttg cctacgtgga gccgcccatc cgcgcgcaca gcgggttcgt
    10981 ggacaaatac atgggcgacg gcatcatggc gctgttcgag aacggcgacg ggaacaacag
    11041 ctgcgacaat gcggtggccg cttcgctgga aatgctggaa cagctggaag cgttcaacga
    11101 aaaaagaatt tctgccgggg aagaaccgct gcgcatgggc atcgggctgc acaacggacc
    11161 cgtgatcatc ggaacgctcg gcgggaatga acgcatggat tccaccgcca tcggcgatgc
    11221 agtgaatctt acctcgcgca tcgaaggcat gacgaaaatg tacggcgttc cgctgctggc
    11281 gggtgaaagc atcatccgtg cgctcagcat ccgtcatgaa ttatactgcc gcttcgtgga
    11341 cacggtggtg gcgaaaggaa agaacgaacc tgtcgcgatc tgggaaattt ccgggagaag
    11401 atcggacaag cgtctcgctg cgtatgcaaa gctcattccc gcttatgaag aagcgatccg
    11461 ttttttccgt gaacggaaga tcgccgatgc gaaaaaagaa tttgaaaaat gcctggcgct
    11521 tttcccgggt gacacggttt cccgcattta cctcgaccgc tgcacgaaat tccttgagac
    11581 cggcgttgat gaaaacgtaa gcgggatcac aagcctcgat tcgaaataaa gcatccgggg
    11641 aaaataaatg cggtaccgca aaaacataca ataaaaaaac cgttcttttc gttttcgtct
    11701 aatgcctttg ccgatggact attcttccgc ttcacgattc atgctgaaaa agctggaaag
    11761 tgagctgcct tcctattgtt tttatcacga ctatgcgcat acgctcgacg tgctcgccgc
    11821 cgtggaaaca ctcgcgaggg aagaatccat caccgatacg gaaaccgtag atcttatgcg
    11881 gaccgctgcg gtttaccacg actgcggatt tttcgaacaa taccgcgcga acgaaccgat
    11941 cgccgtgcgc atcgccgcgg agatacttcc acggtacggt tattcggaac cgcaggtgaa
    12001 atttatttcc cggcttattc tggtcaccgc gctcgatgcg gtacctgcgg atatttacga
    12061 gcagatcctg aaagatgccg atttcgatta cctcgggcgg gacgattatc ccgaaactgc
    12121 gctgaagctg aaagaggaat ggagcgtgat gggaatgaat tattcgatgc acgaatggca
    12181 cgggctgcag ctgcgtttcc tggaaagtca tgtttattac acttcaaccg cgcagcgcct
    12241 ccgggggccc ggcaagcaaa aacatattca gaagctgaaa gaaattcttg ctgcggaaaa
    12301 gtaattctcc tcaatgacgc ggaagaaaat cgctttggct ctccatgaaa caacttccgt
    12361 gttaaaaaaa ccacaaagcg cacaatgctg tgacgaagga cacgaagctg tgctaaaaca
    12421 aatcttgcaa agacgcggaa gaaaaaactc tgtggctctc cgtgaaacaa cctccgtgaa
    12481 cttccgtgtt aaaaaaacca caaagcgcac aatgctgtgc acgaaggaca cgaagctgtg
    12541 ctaaaaaaat cttgcaaaga cgcggaagaa aatcgctgcg gctctccgtg aaacaacttc
    12601 cgtgttaaaa aaaccacaaa gcgcacaatg ctgtgcacga agggcacgaa actgcgaaaa
    12661 acaaatttta ccgagacgaa aaaatgcggg agaatgtctc t
//
LOCUS       DASTSF010000421         2755 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_699336,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000421 DASTSF010000000
VERSION     DASTSF010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2755
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_699336"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1481)
                     /locus_tag="VFU15_12165"
     CDS             complement(<1..1481)
                     /locus_tag="VFU15_12165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718586.1"
                     /translation="MKLRILFLFTALAPHLHAQLFQWAHGFGSQYQNNISTSVKYDKQ
                     GDCFISGYFRGTVDFDPGPAVHSLAASGRAMYIAKFDTYGNYAWAIDVDMGGNTYYGF
                     TLDVDSAENVLLSGTFNGTADFDPGPGVYSLTSISNQDGLVAKYDANGNFVWAFTIGA
                     NGGSTFVASDNQADVIVCGGFTGASVDFDPGTGVSTMSTTGNANGTFIAKYDAGGNYL
                     WSKSISSPVQGSSVLAESVSAMPGGDIIVSGFLNYTADFDPGPGVDTVSTSWQNKTFI
                     ARYDANGNFISVFAIQMSEYLHGTKSRVDINGNIFLGGSFQATADMDPGPGVSTLSAL
                     GYCSSFIAKYDPSGNLQWAHSFGHSSTYTAGMSLDCDLAGNVFLTGYFSDTVDFDPGP
                     GVADLISDTARDAYVVRYDSNGNYTGGFNIPTAHGYSIAADWNGSIYLTGTFFSPHSD
                     FDPEPPVYALTPLGQQDFYIAKYASLPLSVEQDSPVNEMTVFP"
     gene            complement(1605..2567)
                     /locus_tag="VFU15_12170"
     CDS             complement(1605..2567)
                     /locus_tag="VFU15_12170"
                     /inference="COORDINATES: protein motif:HMM:NF019284.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HEU4718587.1"
                     /translation="MKKLLLLFSIASSFSLTAGNFFRLTDYPVAANAYAFLPADDTNK
                     TDASGNKNGFWIERTGAFDWEGYYVGGKREGTWAGYPVGLTMPSYIDNYHAGKKNGMS
                     MSIDRAGYISQIATYKDDSLDGVKRAFFTGGRLKSEEYYKNGQLNGKRTIYNSSNKVQ
                     EDGFWVNGKRTGVARWYYSDGKVFAESTYKDGLMNGPSVSYYENGNKATTVNYVNDMQ
                     EGDFNEFYEDGKLKTKGTYVHNLEEGVWITYYESGKIFETGKYVHGKRDGNWSQASED
                     GKTKVKLTYKAGTLVSGKEMQPMPIAPAPVTNPPANNPPPKKGH"
BASE COUNT          641 a          740 c          709 g          665 t
ORIGIN      
        1 tcggggaaaa ccgtcatttc atttaccggc gaatcctgtt caacgctcaa cgggagcgaa
       61 gcatattttg cgatgtaaaa atcctgctgg cccaaaggcg taagcgcgta cacaggcggc
      121 tccggatcga aatcggaatg cggtgagaaa aaagttccgg tgagataaat ggatccgttc
      181 cagtcggcgg cgatggaata gccatgcgca gtggggatat tgaacccgcc cgtgtaattt
      241 ccattgctat cgtaacgaac aacgtacgca tcacgcgctg tatccgaaat aagatctgca
      301 acgcccggac cgggatcgaa atccaccgtg tcggaaaaat agccggtgag aaaaacattt
      361 ccggccaggt cgcagtcaag agacatcccc gccgtgtacg tcgagctgtg cccgaagctg
      421 tgcgcccatt gcaggttgcc cgagggatcg tatttcgcaa tgaaggaaga acaatatccc
      481 agcgcggaaa gcgtgcttac accgggaccc gggtccatat ccgccgtggc ctgaaatgat
      541 ccgccgagaa aaatattccc gttgatatca accctgcttt tggttccatg caggtattcg
      601 ctcatctgta tcgcgaaaac agaaatgaaa tttccgttcg catcatatcg ggcgatgaac
      661 gttttgttct gccagctggt ggagaccgta tcaactcctg ggcccggatc gaaatccgca
      721 gtataattca gaaacccgga aacgataatg tccccgccgg gcattgcaga aaccgattcc
      781 gccagcacgc tggagccctg tacaggagag ctgatgcttt tggaccagag ataattcccg
      841 cctgcatcgt atttggcgat gaacgttccg ttcgcattac cggtagtgga catcgtgctg
      901 acgccggtgc cgggatcgaa atccacggac gcgccggtga atcctccgca gacgataaca
      961 tcagcctggt tatccgacgc gacgaatgtg cttccgccat ttgcgccgat cgtgaaagcc
     1021 cacacgaaat ttccattggc atcgtacttc gccacaagcc catcctggtt cgagatactg
     1081 gtgagcgaat aaacgcccgg acccggatcg aaatctgcgg tgccgttgaa tgtcccggaa
     1141 agaagcacgt tctccgcgga atcaacgtcg agcgtaaatc catagtatgt gttcccgccc
     1201 atatcgacgt ctatcgccca ggcataattc ccgtacgtat cgaacttcgc aatgtacatc
     1261 gcgcgcccgg aagccgccag gctgtgcacg gcaggtccgg gatcgaagtc gacggtaccc
     1321 cggaaatacc cggagatgaa gcaatcgccc tgcttgtcgt atttgaccga ggtactgatg
     1381 ttgttctggt actggctgcc gaagccatgc gcccactgaa acagctgcgc atgcaaatgc
     1441 ggcgccagcg cggtgaagag aaaaaggata cgaagtttca tgatgccgct taaatgtaca
     1501 aaaaccggcg gatcgttttc agccccggca aacaccatgt aaaaaaagca tgaaaaaagg
     1561 ccggtcctgc gacgcggcct ttcattcatt ttttgctgaa aagatcaatg tccttttttc
     1621 ggcggaggat tgttggccgg cgggttcgtc accggtgcgg gagcgatggg catgggctgc
     1681 atttcttttc ctgaaacaag cgtgcctgct ttgtacgtca gcttcacttt cgtttttcca
     1741 tcttccgaag cctgcgacca gtttccgtcg cgcttgccgt gcacgtattt cccggtttcg
     1801 aaaatttttc cgctctcata ataggtgatc catacgcctt cctcgaggtt gtgaacgtat
     1861 gttcctttcg tcttcagctt tccgtcttca taaaattcat tgaagtcgcc ttcctgcata
     1921 tcgttcacgt agttgaccgt ggtggctttg ttgccgttct cgtaatagct tacgctcggg
     1981 ccgttcataa gaccgtcttt ataggttgat tcagcgaaca cttttccgtc ggaataatac
     2041 cagcgggcaa cacccgtgcg cttgccgttc acccagaacc cgtcttcctg cactttgttg
     2101 ctgctgttgt agatcgtgcg tttcccgttg agctggccgt tcttgtaata ctcctcgctt
     2161 ttgagacggc cgcccgtgaa gaatgcgcgt tttacgccgt ccagcgaatc gtccttgtac
     2221 gtggcgatct gcgaaatgta cccggcgcgg tcgatgctca tggacatgcc gttctttttc
     2281 cctgcgtgat aattatcgat gtaggaaggc atcgtgagtc cgaccgggta gcccgcccag
     2341 gttccttcgc gttttcctcc gacgtaataa ccttcccagt cgaacgcacc cgtacgctcg
     2401 atccagaaac cgttcttgtt gccggaagcg tcggttttat tcgtgtcatc cgcaggcagg
     2461 aacgcatacg cattcgcagc gaccgggtaa tcggtcagcc tgaaaaagtt gccggcggtg
     2521 agtgaaaatg aagaggcgat gctgaaaagc agtagtaatt ttttcatggt tttgtaaatg
     2581 aaattaagac ttgtgtttgc gtcgcaattt acagcaaaaa tcgtgctgaa aaacgattac
     2641 ctgcaccaaa atccgggaat tgccttcgga aaacaatccc cgggcagcag caggaaaaaa
     2701 cctttcgccc tttgcccggc cagatgaaaa cataagtcac cggtcgtttt tactt
//
LOCUS       DASTSF010000422         3572 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_809394,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000422 DASTSF010000000
VERSION     DASTSF010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3572
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_809394"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3473
                     /locus_tag="VFU15_12175"
     CDS             <1..3473
                     /locus_tag="VFU15_12175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEU4718588.1"
                     /translation="PPLKVDLDESFADDTATRTNEILDKIDEVVYYVNLHKDGGRTLR
                     FVSNKFEQVLGVSREEYIKRPTVILDHCHPEDQPGIFETAKKLREEKRPQTFVYRYHH
                     PVMHRYIWIEEKVYPQFDAEGNHSANLGIMRDVTDRLEKEWKLIESTIALERVLNTID
                     EVVYHLDLSLPVGERIRFVGSNVEKVFGVSREEFLKDQKNFLSECHPDDLEVIRRIAD
                     EQQREKKPKVFHYRYRNRKTGEYRWIEEKIIPGFDSEGNQTDVFGVARDVTVQSNALE
                     KLRVSEERFRMLAENATDIIYRFLFVPQPHYEFISASVEHILGYSPEEYYSDFNTGER
                     SIHPDDLPKMRHATELMLQNSSKVFSPESEPLLLKNIRKDGQVVWIETRNRPILDEFG
                     KVIGLEGISRDVTQRQEAENELFNSRESYKGLIDNDPNGIVVSGFDQKIIFANKAAVE
                     ISGQDSEEKLIGHTLLEFLSPAQRLVSAERIKEIASGRNVPFEVIEILHPDGKIIKCE
                     SKPVIYNYLGRQSILVFLRNITAERQLEKEQLRLQVAEETNRRLESEIEERKRTEKEL
                     FETQKYLRLLIDSSLDMICASDNEGRIMEFNKAAQEAFGYTREEILGKKVTSLYSDPD
                     QRHGVMKILVEGNGFFSGEVENKRKNGEIFTAFLSASALRDMNGNVIGSMGVSRDITK
                     SKQAENELRLSEEKYRAIYSQAYIGIALIDTDSDKFLDVNQRLCDMLGYSPEELTGKT
                     VFDLRIPGDLSRLPAGSEFIRRGFERIIDEHRYRHKNGSEVIVNVTISLVRGENRKPL
                     YFIYVYEDLTPKRRAEEQIRLQAAKLEAIIESSSHMIWTVDRNFRLTSFNDNQARWLK
                     RNYNATAYIGMPMTSGKMVSEENYNNFWIERLQETLEGKPQNFEVNFVTPSGENTWRE
                     IFLNPIVDENETVVEVSCIANDITDKKRADDNLRLSLKEKEVLLKEVHHRVKNNLQVI
                     SSILNLQSSYVRDKKILEILVESQNRIKSMAFVHESLYQTKDFSNISFQEYVSNIARN
                     LVHSYSSSDSPPELNLQLDPVKLHLDTAIPCGLIINELLSNALKYAFQPGKSGEINLV
                     LRQRKDRIELSISDNGKGLPDDFDFRNTESLGLQLVVSLVEQINGKIKLDTKKGTKFT
                     IEFAGLSGNA"
BASE COUNT         1008 a          911 c          934 g          719 t
ORIGIN      
        1 gtcccccgct gaaagtagat ctcgatgaaa gttttgcaga cgatacagcg acgcgcacca
       61 atgagatcct ggataagata gacgaggttg tttattacgt caacctgcat aaggacggcg
      121 gaagaacctt gcgttttgtc agcaacaaat tcgagcaggt gctcggcgtt tcccgcgagg
      181 aatatataaa acgcccgacg gtcatactcg atcattgtca cccggaagat cagccgggaa
      241 tttttgaaac cgcaaaaaaa ctgcgggagg aaaaacgccc gcagacattc gtataccggt
      301 accatcaccc ggtgatgcac cggtatatct ggatcgaaga aaaagtttat ccccagttcg
      361 atgccgaagg aaaccacagc gcgaacctcg ggatcatgcg cgatgttacg gaccgtcttg
      421 aaaaagaatg gaagctcatc gaaagcacga tcgcgctgga gcgcgtgctc aatacgatag
      481 acgaggttgt ttaccatctt gacctttctc ttcccgtcgg agagcgtata cgtttcgtcg
      541 gcagcaacgt ggaaaaagtt ttcggcgtca gccgcgagga attcctgaaa gaccagaaga
      601 attttttgtc ggaatgccac cccgacgacc ttgaggtcat ccgccgtatt gccgatgaac
      661 agcaacgcga aaaaaaaccg aaggtgttcc attaccgtta ccgcaaccgg aaaacggggg
      721 aatacaggtg gatagaagaa aaaataattc ccgggtttga cagcgaagga aaccagaccg
      781 acgtattcgg tgttgcacgt gacgtaacgg tccagtccaa cgcgctggaa aaactgcggg
      841 tgagcgaaga acgcttccgc atgctggcgg aaaacgcaac ggacatcatt taccgtttcc
      901 tgttcgttcc gcagccgcat tacgaattca tcagcgcatc tgtcgagcac atcctgggtt
      961 attctccgga agaatattat tcggatttta ataccggaga aagatccatt caccccgacg
     1021 accttccgaa aatgcggcac gccacggagc ttatgctgca gaattcgtcc aaggtttttt
     1081 ctccggaaag cgagccgctc ctgcttaaaa atatccggaa agacgggcag gtggtgtgga
     1141 ttgaaacgag gaaccgtcct atcctggatg agttcgggaa agtgatcggt ctcgaaggaa
     1201 tttcccgcga cgtgacgcag cggcaggaag cggagaatga gctgttcaac agccgcgaaa
     1261 gctataaagg actcatcgat aacgacccga acggaattgt tgtttcaggg tttgatcaga
     1321 aaataatttt tgccaacaag gcggcggtgg aaatatccgg ccaggacagc gaagaaaaac
     1381 tgatcgggca tacgctgctc gaatttcttt ctcctgccca gcgcctggtt tccgccgaac
     1441 ggataaagga aatagcaagc ggacgaaacg tgccgttcga ggtgatagag atccttcacc
     1501 ccgatggaaa aataatcaag tgcgaatcca aaccggttat ttacaattac ctcggccggc
     1561 agtccatcct ggttttcctg cgcaacatca cggcggagcg ccagctggaa aaagagcagc
     1621 ttcgcctgca ggttgccgaa gaaacgaacc gccggctcga gtcggagatc gaagaaagaa
     1681 aacgcacgga aaaagagctt tttgaaacac agaaatacct ccgcctcctg atcgacagct
     1741 cgctcgatat gatctgcgct tcggacaatg aagggcggat catggaattc aacaaagcgg
     1801 cgcaggaagc gttcggctat acgcgggaag agatcctcgg gaaaaaagtg acatcgctct
     1861 actccgaccc ggaccagcgg cacggtgtga tgaaaatact ggtggaaggc aacggctttt
     1921 tttcaggtga ggtggaaaat aaaagaaaga acggagaaat atttacggcg ttcctttcgg
     1981 ccagcgccct gcgtgatatg aacgggaacg ttatcggatc gatgggtgtt tcgcgcgaca
     2041 tcaccaagag caaacaggcg gagaacgagc tgcgcctcag cgaagaaaaa taccgcgcga
     2101 tctattcgca ggcatacatc ggcattgcgc tcattgatac cgacagcgat aaattcctcg
     2161 atgtcaacca gcgcctgtgt gacatgctcg ggtattcacc cgaggagctg accgggaaaa
     2221 cggttttcga tctgcgcatt cccggcgacc tgagccgcct gccggccggc agcgaattta
     2281 tccgccgcgg tttcgaacgg attatcgacg agcaccgcta ccgccacaag aacggaagcg
     2341 aggtcatcgt caacgttacg atctcgctgg tgcgcggcga aaacaggaaa ccgctgtact
     2401 tcatttacgt ttacgaagat cttacgccca aacgaagagc ggaagagcag atccgcctgc
     2461 aggccgccaa gctggaggcg atcatcgaga gcagctcgca catgatctgg actgtcgaca
     2521 ggaatttccg tctcacatcg ttcaacgata accaggcccg ctggctgaaa agaaactaca
     2581 acgccacggc ctacatcggt atgccgatga cgagcgggaa aatggtttcc gaagaaaatt
     2641 acaacaactt ctggatcgaa cggctgcagg agacgctcga gggaaaaccg cagaacttcg
     2701 aagtgaattt cgtcacgcct tccggtgaaa atacgtggcg ggaaatattc ctgaacccga
     2761 tcgtcgacga gaacgaaacc gtggtggagg tttcctgcat cgccaatgac attaccgata
     2821 aaaaacgcgc ggatgacaac ctgcgcctct cgctcaagga gaaggaagtg ctgctgaagg
     2881 aggttcacca ccgcgtgaaa aacaacctgc aggtaatatc gagcattctc aatctccagt
     2941 cgtcgtatgt gcgcgacaaa aaaatcctcg agattctcgt cgaaagccag aacaggatca
     3001 aatcgatggc cttcgtacac gaaagtcttt accagacaaa agacttttcc aacatcagct
     3061 tccaggaata cgtgagtaac attgcgcgga atctcgtgca ttcgtattcg tcttcggatt
     3121 ctccgccgga gctcaacctc cagctggacc cggtgaagct gcacctcgat accgccattc
     3181 cctgcggact tatcatcaac gaattgttgt ccaacgcgct caagtatgcg ttccagccgg
     3241 ggaaaagcgg tgagatcaac ctcgtgcttc gccagcggaa agaccgcatc gagctcagca
     3301 tttcggataa cgggaaaggg ctgcccgatg atttcgattt cagaaacaca gaatcgcttg
     3361 gattacagct ggtcgtttcg ctcgtcgagc aaatcaacgg caaaataaag ctggatacga
     3421 aaaaaggaac taaattcacc atcgaatttg ccggcctttc cggtaacgcc tgatcaagaa
     3481 aatgtccaaa accaacattc tcatcgtcga agacgaaagc attgtcgcca aagacattca
     3541 gatgagtctt cgcaagctcg gttacaacgt gg
//
LOCUS       DASTSF010000423        10307 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1813989,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000423 DASTSF010000000
VERSION     DASTSF010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 10307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10307
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1813989"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(28..471)
                     /locus_tag="VFU15_12180"
     CDS             complement(28..471)
                     /locus_tag="VFU15_12180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011071234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU4718589.1"
                     /translation="METCAGNIRINDHREESDFAFVHREIAQSYWAKGIPADIMKKAM
                     DHSICFNVFIDGKQAGFARVITDKATYAYLCDVVITESQRGKGLGKMLMRFIMDHPDL
                     QGLRKFALGTHDAHGLYEKFGFCVAEHPERLMEIRVQDIYLKKKQ"
     gene            complement(477..821)
                     /locus_tag="VFU15_12185"
     CDS             complement(477..821)
                     /locus_tag="VFU15_12185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013550219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718590.1"
                     /translation="MKEKTYLPVLSRKTFYRKLRRNFIYALVMLFISLSIGMGGYHSI
                     CNLSWIDSLLNACMILTGMGPVNAMTTSGAKLFASFYALFSGVAFLTTVGMVLAPIIH
                     RMMHFFHMEEEE"
     gene            complement(825..1709)
                     /locus_tag="VFU15_12190"
     CDS             complement(825..1709)
                     /locus_tag="VFU15_12190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718591.1"
                     /translation="MKKFFITLIPLLTGAMLSAQLPECDIWVFDFSSYNSRFTFRNGF
                     NVTDHKGYDNQPCFSESGSYMLWTSQRDSLGTDIYRYDFSNKAIKRITQTAYSEYSPT
                     YMDGNKYISAVVVEKDSVQRLWKYNKMTGEGTVLMPRVTQVGYHCWYDSRTVFLFKVT
                     EPSTLEIANAASGVTKVCAGNVGRCMQVWHSPKAKLLLYEIDDGKGNFTIRALNGSGM
                     NVADFAPVALPEGCQDFALDANDNIFAAKEAKLYQWKIGSSTEWALAADFGGNGLHSI
                     TRIAISSSGHKIALVDNN"
     gene            complement(1706..2326)
                     /locus_tag="VFU15_12195"
     CDS             complement(1706..2326)
                     /locus_tag="VFU15_12195"
                     /inference="COORDINATES: protein motif:HMM:NF016207.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HEU4718592.1"
                     /translation="MYIPPHFRNENEKELTAFITHHPMAMIASNGETVPDVTHLPFHL
                     VQSGGKQFLESHFSAANPHAGTLSENAEVIVIFSGPNAYISPVHYEKPQNVPTWNFIA
                     VHVHGKIHFHTEEDRSLEILRNTIGAHDPAWLENFNALDTSYLSAMIKGIRAFSVEIT
                     AFEGKYKLSQNRTGSERKNITDHLSLFPEGAPQQEISGYMSRNKKP"
     gene            2406..3698
                     /locus_tag="VFU15_12200"
     CDS             2406..3698
                     /locus_tag="VFU15_12200"
                     /inference="COORDINATES: protein motif:HMM:NF037015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEU4718593.1"
                     /translation="MRKILPVIFLLGFAAFSCKKDNETTSAPVVQFISPAQGAQVNVT
                     DTICIQLHLADESGLVYVDVKLTDQQLGPVASTVRANLSGTSMDVTIYYIIDNHRLPS
                     GAYYLVAEAHNGANFTRQYLSINITGIPRATKGFFAATLPASGTVDVYKCDTSWSASA
                     FGYFSSDFTDMAVSSYWQQLYIDGYSFGLLRALNLDTTSSSWSVSPVITNGASWGPMS
                     VYGSRLWVAQPGYSAFHSYDEAGAVKYTGTGDNGYAPLHLLQSGDRIFSEQKDVASSN
                     VKMVVYSSAGGRLQETNAGLNVVDMFAKSDNEVFVVGNSGAQGKLLIYDYASNGFWEP
                     ITLPAGRVTSATQVDSTTLLIAMDNSNIYKFTYSPVGLLTWKSGVNAQQVRYNDVDNS
                     VLSAEGVNVNVYDYSGAAMQHTVSLPDSAKDVEWWFNR"
     gene            complement(3734..4687)
                     /locus_tag="VFU15_12205"
     CDS             complement(3734..4687)
                     /locus_tag="VFU15_12205"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006795669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HEU4718594.1"
                     /translation="MNYLDFEKPIAELVELRDKMLQTAEKSKADISASLKDIDEQIFA
                     KRKEIYGNLTPWQRVQVSRHPDRPYTLDYIKALCGDSFIELHGDRTVKDDKAMVGGFG
                     SLEGRSVMFIGQQKGRNTKLRQFHNFGMANPEGYRKALRLMKLAEKFGKPVVTLIDTP
                     GAFPGLEAEERGQGEAIARNLLEMSRLKVPVICIIIGEGASGGALGIGIGDKVLMLEN
                     SWYSVISPENCSTILWRSWDNKEKAAVALKLTADDMMSQGLVDGVIREPVGGAHTNWE
                     ETFASVKAEILSQLDELGKKKVNDLVKTRIDKFCHMGVVIE"
     gene            5571..6950
                     /locus_tag="VFU15_12210"
     CDS             5571..6950
                     /locus_tag="VFU15_12210"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEU4718595.1"
                     /translation="MFIRKIAYWLNFTVARWQLIVFFIFLLVAFHYIGDYFAPDDSPT
                     EHNLHVIMQLTLKFISTTLLAVLGFSILTASAAWAYFFFHTRKRDLGLRITFGDDETT
                     EAGWVPVRVSLRGLLRPFLGNVRARIVFSGMRTSPLFLLNENLYERKKLLRYGVSGQG
                     RTLLNDRGIYDVEEVQFQFTDILGIIALPVTIHFTKQLITLPRAQQPLSAKAEPQATE
                     EQTERIEIPKRVVGEYLNYKDFETGDDVRRIVWKIYARNGELVVRIPETMDPYASQLY
                     FYVSYFRETSSPDEGIYLAELLNRYKDKVRNLYETLEKNGFEMRMPADQQLPVIGEEE
                     GKPEMLRITSASWQKNMPPSSFIAPRNAAFVCLSSLVAPAEVYRLLGMIPAYIPVAVV
                     RLSEAIPSPFRMHMKDLFIAPSPRPYDHLRRPWLLSPLRSELKKNEREIAGMLAVRGN
                     GWLVSPGNE"
     gene            6947..9694
                     /locus_tag="VFU15_12215"
     CDS             6947..9694
                     /locus_tag="VFU15_12215"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HEU4718596.1"
                     /translation="MNGKANTDRRTFLQLLMRWLFILVPTLAASYFVLRAIDLRYTSL
                     DTDLGLQALFFGAGLAVAYSLYFFRVRFLVTFLLIWIAWFVTGKIISNVSGEFDYFLV
                     KTEFDLFSTLFIGGWITGFLLERFKPMRIILPLLFLSAITTILVLTLKSEDATAENIL
                     LAIAPFVAYALYMMLAAEQLASMLDADGKKIGYLLLRTTLFLAILLGIVWLSRPDDMK
                     MQMIRKLLQQVESQDNKGNGNNGPYDEKNGLLERGPNGDKQKNGGKDDTNGGGYRLKD
                     TMSMGKKQSQADYLMFCSKLNNYFPDGSPKPLYFVYHYLTKYDPAKESFIRDPEMPYF
                     DEFNVDPSSIPLYRSKTDSTPIKNSFATLMRKDVTAEVYISSNTWSHALLAPGGAYFC
                     ETIPVDTSFGKLFTSAYRVKCYASDLNNAYFVYNPSANPALDEIQQQRFAELRNVKSY
                     AGTDSLLYSYYTAMPSGALYDSITALTQRITKDAVTPVDKVIAIRNYFLQKDKNGNRI
                     FRYSLDAGSNSDPNIPNASMLRNFLFKTHVGYCTYYAGASMLMLRSAGIPSRFTTGFA
                     TIDRSNKNRGWYWFYASQAHAWTQVFFPGYGWMDFDMTIGNEDQKEAPRPDGTPPLPP
                     PEPWLVAIGKAATAPDLQSKHLGLDFNRIIFFNEDYRLDTTFTREVDASVCRVVYGKK
                     DTTLSAIHPGDSILVVSYDDAAKEVPVPSKTIKIETQVKAFPKPIIADEIHIIPKESA
                     EEKKQEEMKKQEKSAQGTTLAAIFLRLAYIAGGIIVLIMIFPLLWLFGLLFRSAMARD
                     PKEKAYRVYRLALYAYHMAGTYRGEETPLEYAENEIDPVYKNDFAGFMRMYLGLKYSG
                     AAADARTTGITKRFRSGLFASIRKKKGFFPAATAYFYPWRANHYFRRRIDRNEEKPEQ
                     P"
     gene            9691..>10307
                     /locus_tag="VFU15_12220"
     CDS             9691..>10307
                     /locus_tag="VFU15_12220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009051864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU4718597.1"
                     /translation="MTEQTHISVNAEKLALLTMNIGRIIRGKQKEIKYVITALISRGH
                     ILLEDNPGAGKTQMAKALAQSISGGLDEAPLNPEGTVAFRRIQFTPDLLPMDLIGSHI
                     YDESKKEFIFKKGPLFTNVLLADEINRASPKVQSALLECMAENQISVGDRTWHLDKFF
                     FTIATQNPIEMEGTYPLPAAQLDRFFMKISFGYVTEDVELDIYND"
BASE COUNT         2274 a         2734 c         2726 g         2573 t
ORIGIN      
        1 gcccgtcgtc cctggcccgg gatctcccta ttgttttttc ttcaggtaaa tatcctgcac
       61 gcgtatttcc atcaggcgtt cgggatgttc agccacgcag aaaccgaatt tttcatacag
      121 cccgtgcgca tcatgcgtgc cgagcgcgaa tttgcgcagt ccctgcaggt cgggatgatc
      181 catgatgaag cgcatgagca ttttcccgag tcctttcccg cgctgcgatt ccgtgatgac
      241 cacgtcgcag agatacgcgt atgtcgcttt gtccgtgatg acgcgtgcga agccggcctg
      301 ctttccgtca atgaaaacat taaagcagat ggaatggtcc atcgcttttt tcatgatgtc
      361 cgccgggatc cctttcgccc agtagctttg tgcgatctcg cggtgaacga atgcgaaatc
      421 actttcttca cggtgatcgt tgatgcggat atttccggcg caggtttcca ttgcggttac
      481 tcttcttctt ccatgtggaa gaaatgcatc atgcggtgaa tgatcggtgc gagcaccatg
      541 cccacggttg tcaggaaagc cacgccgctg aataatgcgt agaatgaagc gaataatttc
      601 gcgcccgagg ttgtcatcgc gttcaccggg cccattcccg tcaggatcat gcacgcattg
      661 agcagggaat cgatccacga aaggttgcag atgctgtggt atccgcccat tccgatggac
      721 agggaaataa aaagcatcac gagcgcgtag atgaaattgc ggcgaagctt ccggtaaaat
      781 gttttacgcg aaagaaccgg gagatatgtt ttttctttca tgtctcagtt gttatccacc
      841 agcgcgatct tgtgacccga gctgctgatg gcgatgcgtg tgatcgaatg caggccgttc
      901 ccgccgaaat ccgctgcgag cgcccattcc gtgctgctgc cgatcttcca ctggtaaagc
      961 tttgcctctt tcgccgcgaa aatattatcg ttcgcatcca gcgcaaaatc ctggcagcct
     1021 tccggcagcg ctacgggtgc gaaatcagcg acgttcattc cagaaccgtt cagcgcgcgg
     1081 atcgtgaaat ttcctttccc gtcgtcgatt tcgtacagca gcagcttcgc tttcggggaa
     1141 tgccagacct gcatgcagcg gcccacgttg ccggcgcaga ctttcgtcac acccgaagca
     1201 gcgttcgcga tctcgagcgt ggaaggttcc gtcactttga acaggaacac cgtcctgctg
     1261 tcgtaccagc aatgataacc gacctgcgtt acacgcggca tcagcaccgt tccttctccc
     1321 gtcattttgt tgtatttcca caggcgctgc acgctgtctt tttccacgac aaccgcggaa
     1381 atgtacttgt tgccgtccat gtacgtgggc gaatattcgc tgtacgccgt ttgtgtaatg
     1441 cgtttgatag ccttgttgct gaaatcgtag cggtaaatgt ccgttccgag gctgtcgcgc
     1501 tgcgaagtcc agagcatgta cgatccgctc tccgaaaagc agggctggtt gtcgtatcct
     1561 ttatgatcgg taacgttgaa cccgttgcgg aatgtgaacc ggctgttgta agaggaaaag
     1621 tcaaagaccc agatatcgca ttcgggcagc tgcgcggaaa gcatcgcccc ggtgagaagg
     1681 ggaatgagcg tgatgaaaaa tttcttcatg gttttttgtt gcgtgacatg taaccggaaa
     1741 tttcctgttg cggagcacct tccgggaaaa gcgaaagatg atcggtgatg ttttttcttt
     1801 cgctcccggt gcggttctgg ctcagcttgt attttccttc gaacgcggtg atctccactg
     1861 aaaatgcccg aatgcctttg atcatcgcgg aaagatagga ggtgtcaagc gcgttgaaat
     1921 tttccagcca ggccgggtcg tgggcgccga tcgtattccg gagaatttcc agcgagcggt
     1981 cttcttccgt gtgaaaatga atttttccgt gcacgtgcac ggcgataaaa ttccaggtgg
     2041 gcacgttctg cggtttctcg taatgaacgg gggaaatgta agcgttggga ccggagaaaa
     2101 tgacgatcac ttccgcattc tcactaagcg tgcctgcatg aggattcgca gcggaaaaat
     2161 gactttcgag gaattgtttc ccgccggatt gcacgagatg gaacggaaga tgcgtgacat
     2221 ccggaaccgt ttccccgttg ctcgcgatca tcgccatcgg gtgatgcgtg atgaacgccg
     2281 ttagctcttt ctcattttca ttgcggaaat gcggaggaat gtacatctgt tcaaaattag
     2341 tgaaaagaga tcgtggctgc cttggccggc cagtcttcct gctgttaatt ttgtaactta
     2401 ctcttatgcg taaaatactt cccgtcattt ttcttctcgg ctttgctgcg ttttcctgta
     2461 aaaaggataa cgagaccact tcagcaccgg tcgttcaatt catttcaccc gcgcagggcg
     2521 cgcaggtgaa tgtgacggac acgatctgca tacagcttca cctggcggat gaaagcgggc
     2581 tcgtgtatgt agacgtcaag cttacggacc agcagctcgg acctgtagcc tctacggtga
     2641 gggcgaacct cagcgggacg tcgatggatg ttaccattta ttacatcatc gataatcacc
     2701 gtcttccttc cggagcctat tatcttgtag cggaagcgca caacggagcc aattttacgc
     2761 ggcagtatct ctccatcaac atcaccggca ttccgcgtgc gacgaaaggt ttttttgccg
     2821 ccaccctgcc cgcgtccgga acggtagacg tgtataaatg cgatacatcc tggagcgcgt
     2881 ccgccttcgg ttatttttca agtgatttca ctgacatggc cgtcagctct tactggcagc
     2941 agctgtacat cgacggctat tcgttcggac ttctgcgtgc gctgaacctc gacaccactt
     3001 cgtcttcctg gtcggtatcg cccgtgatca cgaacggcgc gagctgggga ccgatgtccg
     3061 tctacggatc acggctctgg gtggcgcagc cgggctacag cgcttttcat tcgtacgatg
     3121 aagcgggcgc ggtaaaatac acgggaacgg gtgacaacgg ttatgccccg ctgcacctgc
     3181 tgcagagcgg agatcgtatt ttttccgagc agaaggacgt cgcttcttcg aatgtgaaga
     3241 tggtggtgta cagctcggcg ggcgggcgcc tgcaggaaac gaacgcagga ctgaacgtgg
     3301 tggatatgtt tgcgaaaagt gacaacgaag ttttcgtcgt gggcaacagc ggcgcacagg
     3361 gaaagctgct catctacgat tatgcctcga acggtttctg ggaaccgatc acgctgcccg
     3421 caggaagggt gacgtcagcg acgcaggtcg acagcactac cctgctcatc gcgatggaca
     3481 attcgaacat ttacaaattc acttattcgc cggtgggatt gctcacgtgg aagagtggtg
     3541 tgaacgcgca gcaggtccgc tataacgatg tggataattc tgttctttcc gccgaaggag
     3601 tgaacgtcaa tgtttatgat tattcaggag ctgcgatgca gcatacggta agcctgccgg
     3661 attccgcgaa agacgtggag tggtggttta accggtagaa aattattttt taagcttcgg
     3721 gtttcccggc gcatcattcg atcaccacgc ccatgtggca gaatttatcg atgcgcgttt
     3781 tcacgagatc gttcaccttc tttttgccga gctcatcgag ctgcgagagg atttccgctt
     3841 tcacagaggc gaacgtttct tcccagtttg tgtgcgcacc accgacaggt tcgcggatca
     3901 cgccgtccac aagtccctgc gacatcatgt cgtcggcggt aagcttgagc gcgacggcgg
     3961 ctttttcctt gttgtcccag ctgcgccaca ggatagtgga acagttctcg ggcgagataa
     4021 ccgaatacca gctgttctcg agcatgagca ctttgtcgcc gatgccgatg cccagcgcgc
     4081 cgccggaagc tccttcgccg atgatgatgc agatcacagg aactttcagc cgcgacattt
     4141 cgagaagatt gcgtgcgatc gcttcgcctt gtccgcgctc ttccgcttcg agaccgggga
     4201 atgcgccggg tgtgtcgatg agcgtcacca cgggcttgcc gaatttttcc gcgagcttca
     4261 tcaggcgcag cgccttgcgg taaccttccg ggttggccat tccgaaattg tggaactggc
     4321 gcagttttgt gttgcgtcct ttctgctggc cgatgaacat caccgatctt ccttcgagcg
     4381 atccgaatcc gcccaccatc gctttgtcgt ccttcacggt gcggtcgcca tgcagctcga
     4441 tgaacgagtc gccgcaaagc gccttgatgt aatcgagcgt gtacggacga tccgggtggc
     4501 gtgaaacctg cacacgctgc cacggcgtaa ggttgccgta aatctcttta cgcttggcga
     4561 agatctgctc gtcgatgtct ttcagcgaag cggaaatatc ggccttgctt ttttcagcgg
     4621 tctgcaacat cttgtcgcga agctccacga gctctgcaat gggtttctcg aaatcgagat
     4681 agttcatgtt cagcggtaat ggttgagaga cgcaaaggta cgcatttaca gaatttcggt
     4741 cgcccgcggc ttttttctca ttgctggtta accgttcccg gtttggagtt tcgggtttgc
     4801 ccaaacctga accgcgttga ccgttgttct ccgtgtttcc tctgtgaagc gacctccgtg
     4861 attctctgtg ttaaaaagaa attttcagtt tcgggtttga attgtgtcct ggcctgaacg
     4921 gcgttgaccg ttgttctccg tgtttcctct gtgaagcggc ctccgtgatt ctctgtagtt
     4981 aaaaaatggt tttcagttta tcgtacttcg tttgtcattc cagcttttca actttcaaac
     5041 ctttcaactt tccaactttg aacttgaatt gtttccgttt tccttcgtgc actttgtgaa
     5101 aaacttcgtg ttctttgtgg ttaaaaaatg gttttcagtt tatcgtttcg tttgtcattc
     5161 cagcttttca actttcaaac ctttcaactt tccaactttg aacttgaatt gtttccgttt
     5221 tccttcgtgc actttgtgaa aaacttcgtg ttctttgtgg ttgaaaaatg gttttcagtt
     5281 tatcgcgttt cggttgtcgt ccaacctttc caaccctcca actttgaaca cgaattgttg
     5341 cgcaaagatt tccggcaaag atgccgcggc gctaaaaaag aacccggtac ccattcgggc
     5401 gatagccggg aacagctggc tgtccgggat ttcgtacata agaactggta tattcacccg
     5461 tcactttccg gatcgaatcc caactattgt taatctcctg ccatttattc aaaacgcagg
     5521 ccggggcgtg gccaatattg ttaattttaa ggaggaggca aaatgccttt atgtttatac
     5581 ggaagatcgc atactggctg aattttacgg ttgcccgctg gcagcttatt gtttttttta
     5641 tttttctgct ggtcgccttt cattatatcg gcgattattt tgcgccggac gattctccca
     5701 cggagcacaa ccttcacgtg atcatgcagt tgacgctgaa gtttatctcc accaccttgc
     5761 ttgctgttct cggtttttcc atactcaccg cttccgccgc gtgggcgtat ttcttttttc
     5821 atacgcgcaa gcgcgatctc ggcctgcgca tcactttcgg tgatgacgaa acaacggaag
     5881 ccggctgggt gcctgtgaga gtttcactgc gcgggctgct tcgcccgttc ctcggaaatg
     5941 tgcgggcgcg tatcgttttt tcaggtatgc gcacttcgcc gctgttcctg ctgaatgaaa
     6001 acctgtacga acggaagaaa ctgctgcgtt acggcgtttc agggcaggga agaactttgc
     6061 tcaacgaccg cggcatttac gatgtggaag aagtgcagtt ccagttcacc gatattctcg
     6121 ggatcattgc gctgcccgtg acgatccatt tcacgaagca gctgatcact ctgccgcgcg
     6181 cgcagcaacc gctcagcgca aaagcggaac cgcaggcgac ggaagagcag accgaacgta
     6241 tcgagatacc gaaacgcgtc gtgggtgaat acctcaacta taaagatttt gaaacgggtg
     6301 acgatgtgcg caggatcgtg tggaagatct acgcgaggaa cggggagctt gtcgtgcgca
     6361 tcccggaaac aatggatccg tatgcgagcc agctttattt ttacgtgagc tatttccgtg
     6421 aaacatcttc cccggacgaa ggaatttacc tggcggagct gctcaaccgt tacaaggata
     6481 aagtgcgcaa cctgtatgaa acgctcgaaa agaacggttt tgaaatgcgc atgcccgctg
     6541 accagcaatt gccggtgatc ggtgaggaag aaggaaaacc ggagatgctt cgcattacgt
     6601 ctgcatcgtg gcagaaaaac atgccgcctt cctcgttcat cgcgccccgg aatgcagctt
     6661 tcgtttgtct ttcctcgctc gttgcaccgg ctgaagtgta tcgcctgctc gggatgatcc
     6721 cggcgtatat cccggtggcc gtggttcgtc tttcggaagc gatcccttcg ccatttcgca
     6781 tgcacatgaa agatctgttt attgcaccgt ctcccagacc atatgatcac ctgcgccgtc
     6841 cctggctgtt atcgccgctg agaagcgagc tgaaaaaaaa tgaaagggag atcgccggca
     6901 tgctggcggt gcgcgggaac ggctggctgg tttcacctgg taacgaatga acggaaaagc
     6961 aaataccgac cggagaacgt ttctccagct gctgatgcgc tggctgttca ttctcgttcc
     7021 gacgctcgcc gcttcgtatt tcgtgctgcg cgccattgac ctgcgctata cttcgctgga
     7081 tacggacctc ggcctgcagg cgctgttttt cggtgcagga cttgccgttg cctattcgct
     7141 gtattttttt cgtgtgcgtt ttctcgtcac gttcctgctg atctggatcg cctggtttgt
     7201 gaccggtaaa ataatttcaa acgtctccgg cgagttcgat tatttcctcg tcaaaacaga
     7261 attcgatctc ttctctacgc ttttcatcgg cggctggatc accggttttc tcctcgaacg
     7321 tttcaagccg atgcggatca ttcttccgct gctttttctt tccgcgatca caacgatcct
     7381 ggtgcttaca ctgaaaagcg aagatgccac ggcggaaaat attctcctgg cgatcgcacc
     7441 tttcgtcgcg tatgcgctgt acatgatgct cgctgcggaa cagctggcgt ccatgctgga
     7501 tgccgacggg aaaaagatcg gctacctgct gctgcgcacc acgctttttc tcgccattct
     7561 cctcgggatc gtctggctga gcaggccgga cgacatgaag atgcagatga tccggaagct
     7621 gcttcagcag gtggaaagcc aggacaacaa gggcaatggg aataacgggc cgtatgatga
     7681 aaagaacgga ttgctcgagc gcggaccgaa cggcgacaag cagaagaacg gcgggaagga
     7741 tgataccaac ggcgggggct accggctgaa ggacacgatg tccatgggga aaaagcagag
     7801 ccaggccgat tacctcatgt tctgcagcaa gctgaacaat tatttccccg acggctcgcc
     7861 gaaaccattg tacttcgttt atcattacct cacgaaatac gatcctgcaa aagaaagctt
     7921 catccgcgat ccggagatgc cgtacttcga tgagttcaat gtcgatcctt cgtctatccc
     7981 gttgtaccgt tccaaaaccg acagcacgcc gatcaaaaat tcatttgcaa cgctgatgcg
     8041 gaaagacgta acggctgaag tgtacatctc gtcgaatacg tggagccacg ccttgcttgc
     8101 cccgggcggc gcatatttct gcgaaacgat tcccgttgac accagcttcg ggaaattatt
     8161 cacgtctgca taccgtgtga aatgctacgc gtccgacctg aacaatgcgt atttcgttta
     8221 caatccttcg gcaaaccctg cgctcgacga aattcagcag cagcgtttcg cggaattgag
     8281 aaacgtgaaa agctatgcgg gcaccgattc gctgctgtat tcttactata cggcgatgcc
     8341 gtccggcgct ctctacgatt ccatcacggc gcttacacag cgcattacga aagatgctgt
     8401 gaccccggtt gacaaagtga tcgcgatccg gaattatttc ctgcagaaag acaaaaacgg
     8461 caaccgcatt ttccgctatt cgctcgatgc cggttcgaac agtgatccta atattcccaa
     8521 tgcgtcgatg ctgcggaatt ttcttttcaa gacgcatgtc ggttactgca cgtattacgc
     8581 aggcgcatct atgctgatgc tgcgttcggc gggaattccg agccgcttca ccacgggttt
     8641 cgccacgatc gaccgcagca acaagaaccg cggctggtac tggttctacg ccagccaggc
     8701 gcacgcgtgg acgcaggtct ttttccccgg gtacggctgg atggatttcg acatgacgat
     8761 cggtaatgaa gaccagaaag aagcgccgcg ccccgacgga acgccgccgc ttcccccgcc
     8821 ggaaccctgg ctcgtcgcga tcggaaaagc ggcgacagcg ccggatctgc aatcgaagca
     8881 cctcggcctg gatttcaacc gcattatttt cttcaacgaa gattatcgtc ttgatacgac
     8941 gttcacgcgc gaggtcgatg cttctgtctg ccgggtggtt tatggaaaaa aagacacgac
     9001 gctctccgcc atccacccgg gcgattccat tcttgttgtt tcgtatgacg acgcggcaaa
     9061 agaggtcccg gttccctcga aaacaataaa gatcgaaacg caggtgaagg cattcccgaa
     9121 accgatcatt gccgacgaga tacacattat tccgaaggaa tcggcggaag aaaagaaaca
     9181 ggaggaaatg aaaaagcagg aaaagtccgc gcagggaaca acgctcgcgg cgatcttcct
     9241 gcgccttgcg tacatcgccg gcgggattat cgtgctgatc atgattttcc cgctgctctg
     9301 gctcttcggg ctgctgttca ggtcggccat ggcccgcgac ccgaaagaga aagcgtaccg
     9361 tgtgtaccgg cttgccttgt acgcgtatca catggcggga acataccgcg gtgaggaaac
     9421 gccgctcgaa tatgcggaga atgagatcga ccctgtctac aaaaatgatt tcgcagggtt
     9481 catgcgcatg tatctcgggc tgaaatattc cggcgccgca gcggatgcca gaacaaccgg
     9541 gataacgaag cgtttccgca gcgggctttt cgcttccatc cggaagaaaa aaggattttt
     9601 cccggcggca accgcctatt tttatccctg gagagccaat cattatttca ggagacgcat
     9661 tgacaggaac gaagaaaaac cagaacaacc atgaccgaac agacacacat ttccgtgaac
     9721 gcggaaaagc tggcgctgct gacgatgaac atcggcagga tcatccgcgg aaagcagaaa
     9781 gaaataaaat acgtcatcac ggcgctgatc agccgcgggc acattctcct cgaggacaat
     9841 cccggtgcgg gtaaaacgca gatggcgaag gcgcttgcgc aatccatctc cggcggactc
     9901 gacgaggcgc cgctcaaccc cgaaggaacc gtggctttcc ggaggataca gttcacgccc
     9961 gacctgctgc cgatggacct gatcggctca cacatttacg acgagtcgaa aaaagaattc
    10021 attttcaaga aaggaccgct ttttaccaat gtgctgctgg ccgacgagat caaccgcgct
    10081 tccccgaaag tgcagagcgc gctgctcgaa tgcatggcgg aaaaccagat ctccgttggc
    10141 gaccgcacgt ggcacctcga taaattcttt ttcaccatcg ccacgcaaaa cccgatcgag
    10201 atggaaggaa cgtacccgct cccggccgca cagctcgacc gtttcttcat gaagatatcg
    10261 ttcggttatg tgacggaaga tgtggagctc gacatttaca atgatta
//
LOCUS       DASTSF010000424         9827 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_838397,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000424 DASTSF010000000
VERSION     DASTSF010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 9827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9827
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_838397"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..829
                     /locus_tag="VFU15_12225"
     CDS             <1..829
                     /locus_tag="VFU15_12225"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014165245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEU4718598.1"
                     /translation="NVEAIAHKEKPKLLICGASAYSRDWDYERLRKIADSVGALLMAD
                     ISHPAGLIARGILNDPLPHCHIVTTTTHKTLRGPRGGMIFMGKDFENPWGLTTPKGEV
                     KMMSALLDAAVFPGTQGGPLEHVIAAKAVAYGEALSESFLKYAVQVVKNASVMAGEFV
                     KRGYHVISGGTDNHCMLIDLRSKNITGKQAENALVKADITVNKNMVPFDDKSPFVTSG
                     IRVGSPAITTRGIKERQIPKIVSLMDEVIMNHGNEKKIAAVKKEVNAMMKKYKLFAW"
     gene            complement(874..1527)
                     /locus_tag="VFU15_12230"
     CDS             complement(874..1527)
                     /locus_tag="VFU15_12230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718599.1"
                     /translation="MKTLLAFVMILLTPAFLFGGDGRDTVPAGIITVRRPLVKADITV
                     RSIVFYPDSSRRSNMHVAKVSRHEILMSDTLDEKFPPKVFDPAHYGGDFNPGTVLKYY
                     PADSADYALLFRDLDFYFSYADTVKSDSARFEIIVRKDGTSAVKPLPWKQMDTSSVRL
                     QNTAKTEIQKHPYWFPARRHKKPRSRKMKRVDSVIILVVYARDPAYGRLLPMEIIDK"
     gene            complement(1584..2180)
                     /locus_tag="VFU15_12235"
     CDS             complement(1584..2180)
                     /locus_tag="VFU15_12235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718600.1"
                     /translation="MKRPRVPAAYSLKFKYDYSKEWNRKRFWESRSKTAQRGYKAWLS
                     DPFPPQPVSVRHNEGDSVNALADSTQMESFFKANDRPAGVFPWQEYLATTGYSFAWSD
                     SVKIDSARVQFLVNSDGSVVFTPMPWKTTDSTSRQFEQVVYTLMRKFWIWYPSQQMDN
                     RNSKLRNIPCKVTITIYAVDKTVYEPGATQVPVVPDFH"
     gene            complement(2360..3070)
                     /locus_tag="VFU15_12240"
     CDS             complement(2360..3070)
                     /locus_tag="VFU15_12240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718601.1"
                     /translation="MNKFSLTVVLFLFLSALHAQDTARDVQPGMIMVKKPAIHPYIMI
                     EYSLYLSDVKKTYILVPDPFDSAYMARKMVPVFDSSRYTDAEKRVYPQKKESIARYYA
                     ESLAYRYLPSDTPMVDTMYVDMWIDNRGKIKWVSPDTNYTGAMPEELKKELAWISLSL
                     NDWGTGGGYMTPKKLFHPPQYVPGNYYCEMKIIVSSGPVTAAQKTSGATYAPFDIPLN
                     SPATDEQMDDFIMQNTKK"
     gene            complement(3088..4818)
                     /locus_tag="VFU15_12245"
     CDS             complement(3088..4818)
                     /locus_tag="VFU15_12245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718602.1"
                     /translation="MKKFKINIDRPKPSSDEILSRRNFDNLMNMYNAVPGKVVHKPFW
                     KSGWAISSFAAAAVIATVIAVYVNRTDVSENKTEPQQVVNTNVQPGNNAPVTNNTAAN
                     TVQKRHVAPPLGGLNVPYASYSVNSSNGGVFTHPSGSKLTIPANAFVDENGNHVTGNV
                     EIRYREFRDPVDVFLAGIPMDYDSAGTTYQFESAGMMEVAAFVNGKVVYLDKNKNVQV
                     EFASNNNDPKFNLYRFDTTAGNWVYEGKDKVQPSGTNDNTRHFTNNQVVTNAQMQHYA
                     AVRDSSVQAAAKNYPVPAEPVKPLKADKTKNRFFVGFDSREFPEMAAYKDAMFEVDES
                     KQKFDRNNYNITWDDMALSRGDKEGKYVLTLKKGLQVVKLDVYPVFDGDKYDKAMNVY
                     DQKFAEYQQALNQRTNAEAAARDQYTRMCSGLSMSGSSNYYGTDNSRASQDKSSEVMR
                     MFTISQFGMYNCDVPEMLPQGARVDLSANDANGNLMSGFYTMYHVDREKFSLYSYHNM
                     NPLAGFSFNPKSSNLVWAVKDGALYYADNDQFASMKHSGKTAVEMKPVGRDFASADDM
                     KKFFGIAAKD"
     gene            complement(4843..5427)
                     /locus_tag="VFU15_12250"
     CDS             complement(4843..5427)
                     /locus_tag="VFU15_12250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018342243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU4718603.1"
                     /translation="MEVISKYHATREEMLQEQQLVEEAKRDPAQFRPLYNRYYERIFL
                     FIWQRVDDKEVAFDITSQVFLKAMLNLHKYEFKGVPFASWLYRIAKSEVYTMFRKQQA
                     NRTVNIDTPGINLMADELEDDKYAPYIEIIKEAVTELEEEDMQFIEMRFFEKRPFREI
                     AEIFGITENNAKVKTYRILEKLKKILLDKKKKSA"
     gene            complement(5629..>9827)
                     /locus_tag="VFU15_12255"
     CDS             complement(5629..>9827)
                     /locus_tag="VFU15_12255"
                     /inference="COORDINATES: protein motif:HMM:NF044618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEU4718604.1"
                     /translation="TVGNTFVLLDFDQICKIEFSDAATIEVSNDNGNTWTQLTYTQYL
                     GTAPFQALGNRFSSAAYSNWVPGNNAAIPDNIWWHHETFDVSSLLSNASQCMVRFILT
                     DANANGANGNYGWILDALKVTAAASELYPPVITPLNPIYAGNVFNLGPYTVNDSITDA
                     SGVASATLFYSVNSGPVQNVAMTNVSGNHWQGMIPAANDSDTICYSVQAWDASPASNT
                     AVWPASGCQQFIVHAGITFPYFDNFDGTTLFTANNISTTSQWQLGTPAYGSTNSAHSA
                     PNAWDVNLTTAYDVNADCELYSPVFDFSSVQNARLSFWHNWNCEAYYDGCHLEYTTDG
                     NTWNVLGTVGDPLGTNWYTNATVYSSNTPGWDGASGGWIKSEYILSALNNVVGPVQFR
                     FVFTSDASVVYDGYSIDDFLIRTPSPQDGAVTGVVSPDISSCLPQGNVPLSIAVLNDG
                     TQPIVGPFDITYILDNGTPVTEQFPGTIQPGVTDTFTFVTPLNNTAGFHNLVVYSSVP
                     GDGWNQNDTLAVSYNTAPGVTVPYFNGFENGPSSMNDFCLTTGAQGQIQQLSAAANTG
                     TSGMAFDATSYLGWTFGMDTIPTSANYIWDPANSASHDANARLVVNTAGYNNLVLEFD
                     AKLLYQYANEYTNFRVKVNGTMITPHLQPNNASTPYDSYRYDISSFLPAQFLTIDFEA
                     KCTYDLATTGTGVYLDNIKIYRPDSIDVGVSQITQPVVQSMANTATQVVVKIKNYGYS
                     AQTSIPVAYQVDNNPPVVETWTGNLLPNTTTTYTFTTTFNSPAGPYNLCSWTQLAQDT
                     ALWNDTTCHSMMGMPVFPVPFSDNFDGPNQFVAQTTYTPSWELGNPVTPYITGTHSGP
                     NAWEVNLNGPYANGSNEILYSPFFDFSQATNVELRFWQWYYCDNYYDGGRVEYSVDGG
                     NTWTTLGTYLDPNGTAWYNQQYLISSQQAGWSGNGGGYFQSKYNLAMFDNYPNPVQFR
                     FVFTSDNTSFGPTDGWAIDDFELFMPINAGTDNITFNNPSPLPLPGNNTVRCEIKNTG
                     TIPLVNVNAMLNVDNTTIVTDPVTFSPPLQPGQQATHTFSQPWTGASPGMHTVSVWTS
                     NPNGHGDVDLSDDTTIWNISVMDTFATYPYCNDFETSNGIPPLTTMNGIRFTNTQNSF
                     AQGTPAKNIINNTHSGNTCWVTDLTLNYLRNDSSAIFSPVFTVDTIDCYHLEFWTRYL
                     TEVNHDGGVVEYSYDLGQTWNRLGIAYEPSWYMNSAATGLGSGYQPNFGGTSNGWVKM
                     EHDFRFTQAGPVVFRFRFGSDNAIESEGWAIDDICFSQLPPCVMSVQDVSSDAVSADA
                     YPNPSGTTTNINYNLPDNGHVHISLVNMLGQEVDSYDGTGHAGTNTWTVDVSSLPEGV
                     YFYELTFGTQKIVQKLVVSH"
BASE COUNT         2237 a         2554 c         2628 g         2408 t
ORIGIN      
        1 caacgtcgaa gcgatcgcgc acaaagaaaa accgaaactg ctcatctgcg gcgcttccgc
       61 ttattcacgt gactgggatt acgaacgcct gcgaaagatc gccgacagcg tgggagcttt
      121 gctgatggcg gacatttccc acccggccgg cctcatcgca cgcggtatcc tcaacgatcc
      181 gctgccgcat tgccacatcg tgacaacgac aacacacaaa acgctgcgcg gtccgagagg
      241 cggaatgatc ttcatgggaa aagacttcga aaatccgtgg ggactcacta cgccgaaagg
      301 agaagtgaag atgatgtcgg cgctgctcga tgcggctgta ttccccggca cacagggcgg
      361 tccgctcgag catgtgatcg ccgccaaagc cgtcgcgtac ggcgaagcgc tttccgaaag
      421 cttcctgaaa tacgctgtgc aggtggtgaa gaacgcttcc gtgatggccg gtgaatttgt
      481 taagcgcggt tatcatgtta tttcgggcgg aacggacaac cactgcatgc tgatcgatct
      541 ccgttcgaag aacatcacgg gcaagcaggc cgaaaatgcg ctcgtgaaag ccgacatcac
      601 cgtcaataag aacatggttc cgttcgacga caaatcacct ttcgtcacgt ccggcattcg
      661 cgtaggatcg cccgccatca ctacgcgcgg aataaaagaa aggcagatcc cgaagatcgt
      721 atcgctcatg gatgaagtga tcatgaatca cgggaatgaa aaaaagatcg ccgccgtcaa
      781 aaaagaggtg aacgcgatga tgaagaagta taagcttttc gcgtggtaaa atttcccgga
      841 tgaaaccagg acgccgttga gagacggcgt tttttatttg tcgatgatct ccatcggaag
      901 caggcgcccg tacgcaggat cgcgggcata aaccacgaga atgatcacag aatcgacacg
      961 cttcatcttc cttgatcgcg gttttttgtg ccggcgcgca gggaaccagt agggatgttt
     1021 ctggatttcg gttttcgccg tgttctgcag gcggacggat gacgtatcca tttgtttcca
     1081 cggcaaaggt ttaacagccg atgttccgtc ttttcgcacg atgatctcga agcgcgcgct
     1141 gtcggatttc accgtgtcag catagctgaa ataaaaatcc aggtcacgga aaagcaacgc
     1201 gtaatccgcc gaatcagccg ggtagtattt caggacggtt ccaggattga aatcgccgcc
     1261 gtagtgagcc gggtcgaata ctttcggcgg aaatttttca tccagcgtat cggacatcaa
     1321 tatctcatgg cgggaaactt ttgcgacatg catgttgctc cgccgcgacg aatcggggta
     1381 aaagacaatg gagcgaaccg taatatccgc tttcaccaaa ggccggcgca cggttattat
     1441 cccggcggga acggtatccc ttccgtcgcc gccaaaaagg aaagccgggg taagcaggat
     1501 cataacaaac gcaagcagcg tcttcacagc gatgggaatt tatgcagcta agttacgcca
     1561 ggccggccaa ccgggcatgg cgctcaatga aaatcgggca ctacaggaac ctgggttgcg
     1621 ccgggttcat aaaccgtttt gtcgacggca taaatggtga tcgtcacttt gcagggaata
     1681 ttacgcagct ttgaattgcg gttatccatt tgctgggaag gataccatat ccagaatttc
     1741 cgcatcagcg tataaacgac ctgctcgaac tgccgcgagg tggaatcggt cgttttccag
     1801 ggcatcggtg tgaacacaac gctgccgtcg ctgttcacga ggaactgcac gcgtgcgctg
     1861 tcgatcttta cggaatccga ccaggcgaag gaatagcccg tcgtggcaag gtattcctgc
     1921 cacggaaaaa ctcccgcggg gcgatcgttc gccttgaaaa aactttccat ctgcgtgctg
     1981 tccgccaagg cattcacgct gtcaccttca ttgtgcctga cagaaacagg ctgcggaggg
     2041 aacggatcgg aaagccaggc tttatacccg cgctgcgctg ttttgctgcg gctttcccag
     2101 aaacgtttcc tgttccactc tttcgaataa tcgtatttga acttcagcga atacgcggcc
     2161 ggaacgcgcg gacgtttcac cgtgataagc cccggtttca gcgaatcacc ggaaggtgtt
     2221 tgcgcggaga gaacggagca gccggcaagg aaaaaaaaga agctaaaaac ggaaaagcaa
     2281 aaaccgcagg aaacgcaagc gattttcttt tttctttttc cggcagatct gcatcgtgtc
     2341 ctcatgcagc tcatgacttt tactttttcg tgttctgcat aatgaaatca tccatctgct
     2401 cgtccgttgc cggagaattg agcgggatat cgaaaggtgc ataagtcgct cccgacgttt
     2461 tctgcgctgc cgttacgggt cctgaagaaa cgatgatctt catttcgcag taataattgc
     2521 cgggaacata ctggggcgga tgaaaaagtt ttttcggcgt catgtagccc ccgcccgttc
     2581 cccagtcgtt gagcgaaaga ctgatccatg ccagttcctt tttcagctct tcgggcatgg
     2641 ccccggtata gttcgtatcg ggcgaaaccc attttatttt cccgcggttg tcgatccaca
     2701 tgtccacgta catcgtgtcg accatcggcg tgtcggaagg aagataacgg taggcgaggc
     2761 tctcggcata atagcgggcg atcgattctt ttttctgggg gtacactctt ttttccgcat
     2821 cagtatagcg cgacgaatcg aacaccggca ccattttccg ggccatgtaa gcggaatcga
     2881 acggatcggg aacgaggatg tacgttttct ttacatcgct gaggtaaagc gaatattcga
     2941 tcatgatgta cgggtgaatc gccggctttt tcaccatgat catcccgggc tgcacatccc
     3001 gggctgtatc ctgcgcatgc agcgcggata aaaacaaaaa cagtacaacc gttaagctga
     3061 atttattcat gaccgcaaaa aataaaatca gtctttcgcc gcgatgccga agaatttttt
     3121 catgtcatcc gccgaagcga aatcccttcc gaccggtttc atctcaacag ccgttttacc
     3181 ggaatgcttc atggaagcga actggtcgtt gtcggcgtaa taaagcgcgc cgtccttcac
     3241 cgcccatacg aggttagacg atttcgggtt gaagctgaag cctgcgagcg gattcatgtt
     3301 atggtagctg tacagcgaga atttctcgcg gtcgacatga tacatcgtgt agaagccgct
     3361 catgagattc ccgttcgcat cattcgcgga aagatccacg cgtgcgccct gtggcagcat
     3421 ttccggaacg tcgcagttgt acatgccgaa ctggctgatg gtgaacatgc gcatcacttc
     3481 ggacgattta tcctgtgaag cccggctgtt atcggttccg taatagttcg acgacccgct
     3541 catgctgagc ccgctgcaca tccgcgtata ctgatcgcgc gccgccgctt ccgcattcgt
     3601 gcgctggttc agcgcctgct ggtattcggc gaatttctga tcatatacgt tcatcgcctt
     3661 gtcgtactta tcgccgtcga aaacggggta tacgtccagc ttcaccacct gcaatccttt
     3721 tttcagcgtg agcacatatt tcccttcctt atcgccacgg ctcagcgcca tgtcgtccca
     3781 cgtgatgttg tagttgttgc ggtcgaattt ctgtttcgac tcatccactt cgaacatggc
     3841 atccttgtaa gccgccattt ccgggaactc gcgtgaatcg aagccgacga agaaacggtt
     3901 ctttgttttg tccgccttca gcggcttcac cggttccgcg ggaacgggat aatttttcgc
     3961 tgccgcctgg accgagctgt cacgcaccgc cgcgtaatgc tgcatctgtg cgttggtcac
     4021 gacctggttg ttcgtgaagt ggcgcgtatt gtcgttcgtg ccggaaggct gcactttgtc
     4081 ttttccttca tacacccagt ttccggccgt cgtatcgaaa cggtacaggt tgaatttcgg
     4141 gtcgttgtta ttgctggcga attccacctg cacgttcttg tttttgtcga ggtaaaccac
     4201 tttcccattg acgaatgcag ccacttccat catcccggct gattcgaact ggtaagtagt
     4261 tccggcggaa tcataatcca tgggaattcc ggccaggaac acgtcgaccg gatcgcggaa
     4321 ctcgcggtag cggatctcca cgttgccggt tacatgattt ccgttctcat ccacgaacgc
     4381 attggcggga atcgtgagct tcgagccgga aggatgcgtg aacacgcccc cgttcgaaga
     4441 attgacgctg tacgaagcat agggaacgtt aagtccgccg agcggcggcg caacgtgccg
     4501 tttctgaacc gtgttcgctg ccgtattgtt cgtgaccggc gcattgtttc ccggctgaac
     4561 attcgtattc acgacctgct gtggttccgt tttgttttcc gaaacatccg tacggtttac
     4621 gtaaacagcg attacggtcg caatgaccgc tgccgcagca aacgagctga tcgcccatcc
     4681 tgatttccag aaaggcttgt gcacgacctt tcccggtacg gcgttgtaca tattcatcag
     4741 gttgtcaaaa ttcctccgcg acagtatctc atctgacgaa ggcttcggac ggtcgatgtt
     4801 tatcttgaac tttttcatgg cgtgatgcgc agcccttgca gcttacgcgc tttttttctt
     4861 tttgtcgagc agtatttttt tcagcttttc gagtatccgg taggttttca ccttggcgtt
     4921 gttttccgtg atcccgaata tttccgcaat ttccctgaaa ggacgttttt cgaaaaaacg
     4981 catttcaatg aactgcatgt cttcttcttc gagttctgtt accgcttctt taatgatttc
     5041 gatgtacggg gcatacttgt cgtcctccag ctcatccgcc atcaggttaa tgcctggcgt
     5101 gtcgatgttc accgtccggt tggcctgctg cttgcggaac atggtgtaca cttcgctttt
     5161 cgctatccgg taaagccagg aagcgaaagg aacgcctttg aattcatact tgtgaagatt
     5221 aagcatggcc ttgaggaaaa cctgcgaggt aatgtcgaaa gccacttcct tgtcgtccac
     5281 gcgttgccag atgaaaagaa aaatccgttc ataatacctg ttgtaaagag gcctgaactg
     5341 agcaggatct cttttcgctt cctccaccag ttgctgttcc tgcagcattt cctccctggt
     5401 ggcatgatac tttgagataa cctccatatt ggctggggtt aatgttactg accgcatcgc
     5461 aactttctta aagttacttc aataacgcgg gatattgcaa aagatacaga tcgccggatt
     5521 tattgaagaa aaccattccg cagccacgat caagcgtctc ataaacaggt acttaaaaaa
     5581 gaaaatccgt cccgggttac ggaacggatc ttctttagtg cattattttc agtgactgac
     5641 gacgagtttc tgaacgatct tttgcgtgcc gaaagtgagc tcatagaaat aaactccttc
     5701 cggcagcgag gaaacgtcca ccgtccaggt gtttgttccg gcatggcccg ttccgtcgta
     5761 cgaatccact tcctggccga gcatattcac gagtgaaatg tgaacatgac cgttatccgg
     5821 aaggttgtaa ttgatgttcg ttgttgtacc cgacggattc gggtaggcat ctgcagaaac
     5881 cgcatcgctg ctgacgtcct ggacactcat cacgcagggc ggaagctggc tgaagcagat
     5941 atcgtcgatc gcccatccct ccgactcgat cgcattatcc gaaccgaaac ggaaacggaa
     6001 aacaaccggt cccgcctgtg taaagcggaa atcatgttcc atcttcaccc agccattcga
     6061 tgtgccgccg aaattcggct ggtaaccgct gcccaaaccg gtcgctgcgg aattcatata
     6121 ccagctcggc tcgtaagcaa ttcccaacct gttccatgtc tggccaaggt cgtatgaata
     6181 ttccaccact cccccgtcat ggttgacttc agtcaggtaa cgcgtccaga attcgagatg
     6241 gtaacagtct atggtatcca cggtaaacac cggtgagaag atcgcggagc tgtcattgcg
     6301 caggtaattg agcgtaagat ccgtcaccca gcaggtgttg ccgctgtgcg tattgttgat
     6361 gatgttcttc gccggtgttc cctgtgcaaa gctgttctgc gtatttgtaa agcgtatgcc
     6421 gttcatcgtg gtaagaggcg gaattccgtt cgatgtttcg aagtcgttgc aatacgggta
     6481 cgtcgcaaac gtatccatca ccgagatatt ccagatcgtc gtatcgtcgg aaagatcgac
     6541 atcgccgtgc ccgttcgggt tgctcgtcca tacagaaact gtatgcattc cgggggatgc
     6601 gcctgtccac ggctgcgaga acgtatgcgt cgcctgctgg ccgggctgca gcggcgggct
     6661 gaacgttacg gggtccgtta cgatcgttgt gttgtctacg ttgagcattg cgttcacgtt
     6721 gacgagcggg atcgttcccg tattttttat ttcgcatcgc acggtgttgt tgccgggaag
     6781 cggaagcggc gacgggttat tgaaggtgat gttgtccgtt cccgcgttga tcggcatgaa
     6841 cagctcgaaa tcatcgatcg cccagccgtc ggtaggtccg aacgacgtgt tgtcggatgt
     6901 aaatacaaaa cggaactgca cgggattggg ataattgtcg aacatggcga ggttgtattt
     6961 cgactggaaa taaccaccgc catttccaga ccatccggcc tgctgggaag agatcaggta
     7021 ctgctggttg taccaggccg taccgttcgg atcgagatac gttccgagcg tcgtccatgt
     7081 atttccgcca tcgacactgt attcaacacg tccgccgtcg taataattat cgcagtaata
     7141 ccattgccag aaacgcagct caacgttggt ggcctgcgaa aaatcgaaga acggggaata
     7201 cagtatctcg ttgctgccgt tcgcgtaagg accgttgagg ttgacttccc atgcattcgg
     7261 tccgctgtgt gttcccgtaa tgtaaggtgt aaccgggttt ccgagctccc atgaaggagt
     7321 gtacgttgtc tgcgcaacga actggttggg accgtcgaaa ttatccgaga acggaaccgg
     7381 gaagaccggc atgcccatca tggaatggca ggtggtgtca ttccacaacg cggtatcctg
     7441 cgccagctgt gtccatgagc aaaggttgta cggacctgcc ggtgaattga acgtcgtggt
     7501 gaacgtgtag gttgtggttg tattcggcaa aaggttcccg gtccacgttt cgacaacagg
     7561 cgggttatta tccacctggt aagctacggg aattgaagtc tgcgcagaat agccgtagtt
     7621 cttgatcttc acaaccacct gcgttgccgt attggccatg ctctgcacaa caggctgcgt
     7681 gatctgcgac acaccgacat caatgctgtc cggccggtag atcttgatat tatccaggta
     7741 aacgcctgtt ccggttgtag cgaggtcgta ggtgcatttc gcttcgaaat cgatcgtcag
     7801 gaactgcgcg ggcaggaacg aggaaatgtc gtagcggtag ctgtcgtaag gtgtggacgc
     7861 gttgttcggc tgcaggtgcg gcgtgatcat cgttccgttg accttcacgc ggaaattggt
     7921 gtattcgttc gcgtactggt acagcagttt cgcatcgaac tcgagcacga gattattata
     7981 ccctgctgta ttcaccacga gccttgcatt tgcgtcatgt gaagcgctgt tggcgggatc
     8041 ccagatgtaa ttcgctgacg tcggtatcgt atccatgccg aacgtccatc ccaggtaaga
     8101 agtcgcatcg aacgccatgc cgctggttcc tgtattcgcc gctgcgctga gctgctgaat
     8161 ctgtccctgc gcaccggttg taagacagaa atcattcatg ctcgacgggc cgttctcaaa
     8221 cccgttgaaa taaggaacgg ttacgccagg tgccgtgttg tagctgaccg ccagcgtgtc
     8281 gttctggttc cacccgtcgc cgggaacgct ggaataaaca acgaggttat ggaatccggc
     8341 cgtattgttc agcggcgtaa cgaacgtaaa cgtatccgtc actccgggct gtatggttcc
     8401 cgggaattgc tccgtaaccg gcgtgccatt atcgagaatg taggtaatgt cgaaaggacc
     8461 aacgatcggt tgcgtaccgt cgttaagcac ggcaatggaa agcggcacat ttccctgggg
     8521 aaggcagctg gaaatatccg gggaaacaac tccggtcacc gccccgtcct gcggactcgg
     8581 tgtacggatg agaaaatcgt caatggaata accatcgtat accaccgagg cgtctgaagt
     8641 gaacacaaaa cggaactgca caggtccgac cacgttgttc agcgccgaaa ggatgtattc
     8701 cgatttgatc cagcctccgc tcgctccatc ccagcccggc gtattcgaag aatagacggt
     8761 cgcgtttgtg taccagttcg tgcccagcgg atcgccgacc gtaccgagca cattccaggt
     8821 gtttccatcc gtcgtgtatt cgaggtggca tccatcgtaa tatgcttcgc agttccagtt
     8881 gtgccagaag cttagtcttg cgttctgcac gctggaaaaa tcgaaaacag gagaatacaa
     8941 ttcgcaatcg gcattcacgt cgtacgcagt agtgaggtta acatcccatg cgttgggcgc
     9001 ggaatgcgcg gaattcgtag aaccgtatgc cggcgttccg agctgccatt gcgaagtcgt
     9061 tgaaatgttg ttggcggtga acagcgtcgt tccgtcgaaa ttatcgaaat acgggaacgt
     9121 aatgcccgca tgaacaatga actgctggca acccgatgcg ggccatacag ctgtgttcga
     9181 cgccggtgaa gcgtcccatg cctgcaccga atagcagatc gtgtcggaat cattggcggc
     9241 ggggatcatt ccctgccagt ggttaccgga aacgttggtc attgcaacat tttgcacagg
     9301 accgctgttc acggaataga aaagcgtcgc actggcgaca ccgctggcgt cggtgatgct
     9361 gtcgttgacg gtgtacggac cgaggttgaa aacattcccg gcatagatcg ggttgagcgg
     9421 cgtgataacc ggcggataca attcggaagc ggccgccgtc actttgagcg catcaaggat
     9481 ccatccgtaa ttcccgttgg cgccgttggc attggcatcc gtcaggatga aacgcaccat
     9541 gcattgcgaa gcatttgaaa gcagggagga aacatcgaac gtttcgtgat gccaccagat
     9601 gttatcggga atggccgcat tattacccgg gacccaattc gaataagcag ccgaagagaa
     9661 tcggttgccc agcgcctgga acggagccgt gccaagatat tgtgtgtagg taagctgcgt
     9721 ccatgtattc ccattgtcat tcgacacttc gatggtcgcc gcatcgctga actcgatctt
     9781 gcagatctgg tcgaaatcca acagcacgaa cgtatttccc accgtgg
//
LOCUS       DASTSF010000425         4087 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_519100,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000425 DASTSF010000000
VERSION     DASTSF010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4087
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_519100"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(27..1472)
                     /locus_tag="VFU15_12260"
     CDS             complement(27..1472)
                     /locus_tag="VFU15_12260"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011583462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HEU4718605.1"
                     /translation="MKKILSAFAIAVMGGAAALGLNHLFFASPAVSASSAMNPPAHYA
                     NFGSGGAPDFRDAAEQTIHGVVHVTTKFTTQQTYIDPFQSWFFGNGGMQVVPKEQQAS
                     GSGVIISSDGYIVTNYHVIEKADEIEVTLNDKRNYTAKLVGSDPNTDLAVIKIDEKDL
                     PFIPYGNSDDVKIGEWVLAVGNPFNLTSTVTAGIVSAKARNINLISSNGSGAGAVESF
                     IQTDAAVNPGNSGGALVNTSGQLVGINSAIASNTGTFTGYSFAIPVNLVKKIVSDLME
                     YGTVQRGYLGVNIRDVDADLVKDKGLKDTKGVYVESVIAGGSADQGGIQAGDVITKIG
                     DVDVNNVSELQEQVNRFRPGDKLSVSFTHGSKDKTTSVVLKNKDNNTNLEKKDDVADK
                     DDVLGAGFETVAKEDLTKLGIDNGVRVTNLGPGKLRSAGIRDGFIITAIDKNKVTTPD
                     ELKKTLDSKKGGVLIEGVYPDGARAYYAFGM"
     gene            1639..2298
                     /locus_tag="VFU15_12265"
     CDS             1639..2298
                     /locus_tag="VFU15_12265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718606.1"
                     /translation="MKVRSRFDHFRDRTQVFLLGFLLGIVLGGGFFVLKIDQYVKELN
                     FYKSLTQKDDTDDKNVKSDDTVPGKPRTHRTKKENVRTDTAEKAQALQTSGDSVPPSV
                     FSDDDDNIVVKKDELLGQKPFGVIDLKEVKDDSTASVTPGKSVTVEFWKSPLNYRGYK
                     FSRNRLVLFGFETEDVESVYSLDNAIYVKSVTGVFRVDPTTDFRQMERVTDESLIARM
                     K"
     gene            2295..3077
                     /gene="dapF"
                     /locus_tag="VFU15_12270"
     CDS             2295..3077
                     /gene="dapF"
                     /locus_tag="VFU15_12270"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020532806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEU4718607.1"
                     /translation="MRTVFSKYHGTGNDFILIDDREHAFDEKDEARIARLCDRHFGIG
                     ADGLILIRKEEGCDFRMVYVNADGKPSSMCGNGGRCAVKFAADHHIVRKKEVTFLAVD
                     GMHEAVIGEKTIKLRMKDVHGIEDGGDFMFLDTGSPHYVKFSANVPGIDVYSEGKKIR
                     NSDRFRKEGTNVNFIEKKDNVLLVRTYERGVENETLSCGTGATASALVAGVHGISTTP
                     TRCDISTPGGNLSVHFHRSEMHFSDIWLEGPAEFVFKGETEL"
     gene            3074..>4087
                     /locus_tag="VFU15_12275"
     CDS             3074..>4087
                     /locus_tag="VFU15_12275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718608.1"
                     /translation="MKPADSIPNTDRINYLNIGLILISCAVAFFVPFELFLFSYAVLG
                     PAHYLTEISWLHERNYFSKGKYNYVFLVVAGFLLFLVLYFDKLFPHVHLFNRDANLQT
                     GMTTGLVYIAFVSALAMVVLKKPFHRLIAFIVIVASASLAKQGVLLFSVFLPTLIHVY
                     LFTGLFMLYGALKSRSKSGYLSVAIFVLCPLLFVFITPSGMPVSDYALHSYSLFETVN
                     RKFLEVFQPDLFVKDFTSLADYRQYLHKVIYESNAGLMVMRFIAFAYTYHYLNWFSKT
                     SVIKWHNVPKRRLVVIGVLWAAAVAFYFWKYEYGFHVLFLLSFLHVFLEFPLNHVTII
                     GIFK"
BASE COUNT          935 a         1124 c         1028 g         1000 t
ORIGIN      
        1 aaagcagctt ttcacccgac caaaatttac attccgaaag cataataagc gcgtgccccg
       61 tcgggataaa cgccctcgat cagcacaccg ccttttttgc tgtccagcgt ctttttgagc
      121 tcgtccgggg tggtgacttt attcttgtca atggcggtaa tgatgaaccc gtcgcggatg
      181 ccggcagaac gcagcttccc gggaccgaga ttcgtcaccc gcacaccgtt atcgatgccc
      241 agcttcgtga ggtcctcctt cgccacggtt tcaaaaccgg cgccgagcac atcgtccttg
      301 tccgccacat cgtctttctt ctcaagattc gtattgttgt ctttgttctt gagcactacg
      361 ctcgtcgttt tgtccttcga accatgcgtg aacgaaaccg acagcttgtc gccgggacgg
      421 aaacggttca cctgctcctg caattcggaa acattgttca cgtccacgtc gccgatcttc
      481 gtaatgacat cacccgcctg tatcccgccc tggtcggccg atccgcccgc gatcacgctt
      541 tccacgtaaa cgcctttcgt gtctttcagt cctttgtcct tgacaagatc cgcatccaca
      601 tcgcggatgt tcacgccaag ataaccgcgc tgcaccgttc cgtattccat caggtcggag
      661 acgatcttct tcacgaggtt gaccgggatc gcgaatgaat agccggtgaa cgttcccgta
      721 ttcgacgcga tggcggaatt gatccccacc agctgcccgg acgtattcac gagcgccccg
      781 ccgctgttgc cgggattgac cgcggcatcc gtctgtatga aggattccac ggcgccggca
      841 ccgcttccgt tggaagaaat aagattgatg ttccgcgctt tcgcactgac gatgcctgcc
      901 gttacagtgg acgtcaggtt gaacgggttc ccgacagcaa gcacccactc accgattttg
      961 acgtcatcgg aatttccgta aggaatgaac ggaaggtctt tttcgtcgat cttgatcacg
     1021 gcgagatcag tgttcggatc ggaaccgacg agcttcgcgg tgtaattgcg tttatcgttg
     1081 agcgtaactt ctatttcgtc cgctttttca atgacgtgat aattcgtcac gatgtagcca
     1141 tcggaagaaa tgatcacgcc ggaaccggaa gcctgctgct ctttgggaac aacctgcatt
     1201 ccgccgttcc cgaaaaacca gctctggaaa ggatcgatat acgtctgctg cgtggtgaat
     1261 ttcgtggtga cgtgcacgac tccatggatc gtctgctcag cagcgtcacg aaaatcaggc
     1321 gcaccgccac tgccgaaatt cgcataatgg gccgggggat tcatcgcgga agaagcggaa
     1381 acggccgggg aagcaaagaa caagtgatta agaccgaggg cggcggcacc gcccataacg
     1441 gcgattgcaa aagcactcag aatttttttc ataatactga ttttttattg gttaaacatg
     1501 tcatattcaa agttcaaaaa aagaactcca aaatccattc ccgcagtatt tttgtcagtt
     1561 gtttttaaca tgatttaata gcggactgcc ggtttttccg ctcccgcgac tttttcctaa
     1621 tttcgctctg ttccttccat gaaagtccgt tcccgtttcg atcatttccg cgaccgcaca
     1681 caggtattcc tgctgggctt cctgctcggc atcgtgctcg gcggcggttt cttcgtcctc
     1741 aagatcgacc agtacgtgaa ggagctgaac ttttacaaat cactgacgca gaaggacgac
     1801 accgacgata aaaacgtaaa atcagacgac acggtacccg gcaagccgag aacgcacagg
     1861 acgaagaaag aaaacgtaag aacggatacc gctgaaaaag cgcaggcgct gcagaccagc
     1921 ggcgacagtg ttccgccttc cgttttttcc gacgacgatg acaacatcgt ggtgaaaaaa
     1981 gacgaactgc tgggacaaaa gcctttcggt gtgatcgatc tcaaagaagt gaaagatgat
     2041 tctactgcat cggtcacgcc gggcaaatcc gtcactgtcg aattctggaa atctccgctc
     2101 aattaccgcg gttataaatt ctcacggaac cgcctcgttc ttttcggttt cgaaacggaa
     2161 gacgtggaat cggtttattc gctcgacaat gctatctatg taaaaagcgt cacgggcgtt
     2221 ttccgggtcg accctacaac ggatttccgg cagatggaac gggtgacgga tgaatcgctg
     2281 atcgcacgca tgaaatgaga acggttttca gcaaatacca cggaacggga aacgacttta
     2341 tcctcatcga cgaccgggag cacgccttcg atgaaaaaga tgaagcgcgc attgcccgtc
     2401 tctgcgaccg gcatttcggc atcggggcgg acgggctgat cctcattcgc aaagaagaag
     2461 gctgcgattt cagaatggtg tatgtcaatg ccgacggaaa accgagctcc atgtgcggga
     2521 acggcggtcg ctgcgcggtg aaatttgcgg ctgaccacca catcgtccgc aaaaaggaag
     2581 tcaccttcct cgccgtcgac ggcatgcacg aagcggtgat cggtgaaaaa acgatcaagc
     2641 tgaggatgaa ggacgttcac ggcatcgagg acggcggcga tttcatgttc ctcgatacgg
     2701 gctccccgca ttatgtgaaa ttttccgcga acgttccggg catcgacgtg tacagcgaag
     2761 ggaaaaagat ccgcaactcg gatcgcttcc ggaaagaagg aacgaacgtg aatttcatcg
     2821 agaaaaaaga taatgtcctg ctcgtgcgca cgtacgagcg cggcgtggag aacgaaacgc
     2881 tcagttgcgg aaccggcgcc acggcgagcg cactcgtggc cggcgtgcac gggatctcca
     2941 cgacgcccac gcgctgcgac atatccacac ccggcggaaa tctttccgtg catttccacc
     3001 ggagcgaaat gcatttttcg gacatctggc tggaaggtcc cgccgaattt gtttttaaag
     3061 gagaaaccga gctatgaaac cggcagacag cattccgaac accgaccgga tcaattacct
     3121 caacatcggg cttatcctca tcagttgcgc ggtggcgttc ttcgttccgt tcgagctttt
     3181 ccttttttcc tacgccgttc tcggtccggc gcattacctc acggaaattt cctggctgca
     3241 cgaacgcaat tatttttcga aaggaaaata caattacgtt ttccttgtcg tggcgggttt
     3301 cctgcttttt ctcgtgctgt atttcgacaa gctgttcccg cacgttcacc tgttcaaccg
     3361 tgatgcgaac ctgcagaccg gcatgacaac ggggcttgtt tacattgctt ttgtctccgc
     3421 gctggcgatg gtcgtcctga aaaaaccatt ccaccgcctg atcgcattta tcgttatcgt
     3481 cgcgagcgca tcactggcga aacagggcgt gctgctgttc tccgtttttc ttcccacgct
     3541 cattcacgtt tatcttttca ccgggctttt catgctttac ggcgcgctga aaagcagaag
     3601 caaaagcggc tacctgtccg tggccatttt cgtgctctgc ccgttgctgt ttgtgttcat
     3661 tacgccttcc ggtatgcccg tcagcgatta tgcgctgcat tcgtacagcc tgtttgaaac
     3721 agtgaacagg aaattcctcg aagtcttcca gcccgatctt ttcgtgaagg atttcaccag
     3781 tctcgcggat tacaggcaat accttcacaa agtgatctat gaatcgaatg ccgggctgat
     3841 ggtgatgcgt ttcattgcgt tcgcttacac gtaccattac ctgaactggt tttcaaaaac
     3901 gtccgttatc aaatggcaca acgttccaaa gcgccgcctg gtggtgatcg gcgtgctgtg
     3961 ggcggcggcc gttgcattct atttctggaa atacgaatac ggtttccacg tgcttttcct
     4021 gctcagcttc ctgcacgtgt tcctcgagtt cccgctcaac cacgtaacga tcatcggaat
     4081 tttcaaa
//
LOCUS       DASTSF010000426        17654 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_538093,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000426 DASTSF010000000
VERSION     DASTSF010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 17654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17654
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_538093"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..5350
                     /locus_tag="VFU15_12280"
     CDS             <1..5350
                     /locus_tag="VFU15_12280"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HEU4718609.1"
                     /translation="SQQVNITQPSPLLAVVSSTTSACGICNGTASVAVSGGTSPYAYF
                     WTPSNQTNPTATNLCVGSYTVTVTDAAGCTTTAIATINQVVNIIVTSSASSLSCSGAC
                     DGVASANASGGTVPYSYSWAPNGQTTQTILNQCAGSYTVTATDAAGCFNTATVTFIDP
                     PPIVITMTNTNASCNGVCDGTATANASGGTGAYTYSWAPGGQTTQTATGLCAGTYTVT
                     VHDANNCVSTETVTITEPTLITANAVSNPANCTLCDGSINTAASGGTGPYTYSWSPGA
                     QTTPSISNLCPGIYSVTITDNTGCSVVIPISVSNINGPTVQATSTDATCNGTCDGTGN
                     ANVTGGASPFTYDWSPGNPTGDGTPNASGLCAGSYFCQVTDNVGCITFANITITQPTP
                     VSANTSQTNISCNGLTDGSLSATGTGGTGPYTYNWLPGGQNTPAISGLGVGGYTVNIT
                     DANGCTFSQSFSITEPGPISASIASTNVQCFGACNGSATVTVVGGSSPYAYSWSSGQG
                     TSITANLCPGSYTVDVTDVNGCTTNQSVTITQPTQLSTSISSTNVTCNALCNGTATVS
                     ASGGVGGYTYTWSPNGGTTPTASGLCAGAYSVITNDSNGCASVQSVTIQEPPAIVLSA
                     SSGPLSCNGSCNGVASVVASGGTPGYIYNWSPGGQTSASATSLCAGIYTVNVTDASGC
                     SQTATTTVAQPAVLQANTSFTSPSCNNGTNGTASANPVGGTGPYSYLWAPGGQTTSTI
                     TGLSVGTYTVTVHDANGCQDVQTINVTAVSPIALTVASAPASCGNCDGTLSVTPSGGA
                     SPYTYNWSNALPNQPNQTNLCAGIYSVTVTDASGCANSFTLTMNNSGGPTGETVVSND
                     ASCPASCDGSATVTPIGGAAPYTYVWNPGGQTTNSLTNMCAGSYFLQVTDTNGCIRFS
                     PVTINAPQPINANPFVSNATCTGICDGSISLSPTGGTGPYSYSWSPGLQTTASITNQC
                     IGTYTATITDANGCTTTSSSTINPWNTLAAVATSSNTTCSGSCNGTATATVTTGSGPF
                     TYNWSDPLGQTTATATGLCPGSYTVVVADGAGCNTTLNVSLNSPTPITINPAVTPTTC
                     GSCNGSVTLNTSGGIAPYTFQWSNGSTTSTLNGLCAGVYSVIIRDASGCTATYSVNIS
                     ASGGPTAANATVTNATCNGTCNGSASLAPTGGTPPYSYLWVSGGQTTSSVNNLCAGLY
                     FFQVRDSAGCALTDTVRITEPPPIVPNQTIVNTTCGICLGSISLNPSGGSAPYTYNWA
                     PLGQTTSNITLLCAGLYTVTITDAAGCSITQPFPVSNSNSTLVLSTSVSGALCNDSCN
                     GMASVSASGGSAPYTYSWSNGSTNDSITFVCAGTYLVQATDAGGCVSTASVNITEPSS
                     LAGSYLFVQDELCTGVCNGFITAIPSGGTLPYTYNWSDAQTTQTASNLCAGSYTVVIS
                     DANGCSVTQNDSIISPVVLVPGVPLITDATCNNSADGAISYSVSGGSPPYTYSWTGPN
                     GYTSASQNISGILPGNYTLTVTDANGCSISDTLAVGSTMVVLANAGNDTVFCQSGSIL
                     LNGTGSTNAVTYQWVQLPGMNVVGNAATATITPPPGATTYVLIVSNGACTDSDSIVVN
                     SLPPPNADAGPDIEIFTGMNATIGGSPTGPPGSTFAWGPSTGLGDPTISNPVAAPGTT
                     TTYTVVVTDGNGCTGSDTMTLTVVPSIEFPNGFSPNGDGINDVWVIDNIQLFPHCHVE
                     VYNRWGEMLFASDGYNTPWDGRYQGKDLPVGTYYYIIKLNDPNFPDDYTGPITIMR"
     gene            5361..6287
                     /locus_tag="VFU15_12285"
     CDS             5361..6287
                     /locus_tag="VFU15_12285"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HEU4718610.1"
                     /translation="MKKALVFLCSCIAMVSFAQQLPHYSQYMLNDYVMNPAIGGKNNY
                     FIGMSDNRYQWGGITDAPRTYVMSVHGPLKYDHMGMGGQLFTDIVGPTRRTGFYLSYA
                     YHAPLTEHLKLSFGISAGILQFMVDGQKIHLHDPGDNVLTNSLQSVLTPDFGAGFYLY
                     SERFWVGASCLQIQQNKLKFFDYMSNTTSVMTRHYYGMLGYRQPIGNDFVLEPSALLK
                     YVSPAPFQFDVGMRLIYKEKVWLGGSYRNLDAIAAYMGFVVRENILIGYSYDYTTTNL
                     GNYNNGTHEVLIGIKFNKNGVQKPNESQTKFD"
     gene            complement(6610..6801)
                     /locus_tag="VFU15_12290"
     CDS             complement(6610..6801)
                     /locus_tag="VFU15_12290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020595610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HEU4718611.1"
                     /translation="MQNGTVKFFNESKGFGFIKVDGTNEEIFVHATGLTQQIRENDKV
                     TFEVQQGKKGPNAVNVKLA"
     gene            6965..7414
                     /locus_tag="VFU15_12295"
     CDS             6965..7414
                     /locus_tag="VFU15_12295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1569 domain-containing protein"
                     /protein_id="HEU4718612.1"
                     /translation="MKTIFNASDYAGIEERINRLTPSSPALWGKMNVAQMLAHCSATV
                     EMALGDVVLKRLFIGRIIGPLVKKKFLGEEPFKQGNPTAREFTITDPRDFEKEKQRLL
                     GLMKRLHEGGEAGATKHPHGFFGPLTPAQWGQTQWKHFDHHLRQFGV"
     gene            8088..10283
                     /locus_tag="VFU15_12300"
     CDS             8088..10283
                     /locus_tag="VFU15_12300"
                     /inference="COORDINATES: protein motif:HMM:NF012271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HEU4718613.1"
                     /translation="MRPFCFTLIAFFFLCGSSSAQNFPVQLNLQLVPPFSGYLNDYCS
                     PGNENFRVLALFTDFSHPSYDVKLKFKIEGQGISLQNQAWYYAGPFTLEPGVPKMISG
                     DDLFPLMDEDHLDFSGITRAQYDQRKVLPEGFYTITVTAYDYANPLPVKVSNDAVTQA
                     WMLLNDPPATNLPVCGTTVQEVSPQQLTFSWTPMNVTLPSSLAGTDYTFELWEIYPSN
                     SSAGTIVATTAPLYSAVTQQTMLSYSITEPPLVTGREYVWRIRAHDADGRELFRNNGY
                     SQLCTFTWGNANSLLGNSANLTLTAQALTHRQAKCSWDSLSVYSSYRVEFKKTGSGNW
                     FPVTTDHASLRICDLEPETNYEAHVQGIFPDGSPGPWSNVATWATPAQQLLNCGDISP
                     APAQQNFQPLTQANDGMIWQVGQFEMEVTSLSAYTNPFGHYSGYGKVITPFGGITLNC
                     EFTSITIGDDHQMYAGEVKAVSEGVSNWMTQFNAGQFHYDTSWFYNGNIDSIYVNANG
                     EIVLLDENGNTVTIGNNTDGGVLITDANGNQWIVNPDGAVTFVTGDFLLPLTTDTLTA
                     HEMQLLKMAMTIIRSQLSPNDISARQNVFETSEGNLGNYISTQRQSLPSSPGNSGTIT
                     SDSAAFVSYYEKPLNPNDAGAQLGNTYKTAQVDYYSAKVLAVLSRADCPDDELNFIGQ
                     YLTVNGVLYHEYVTQQVALGKTDQQIADEVAENGVKQLVVMTLRKQMSR"
     gene            10314..12992
                     /locus_tag="VFU15_12305"
     CDS             10314..12992
                     /locus_tag="VFU15_12305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718614.1"
                     /translation="MNARNKRHGAGRKAMIVFAIVLQAVFCIRGKALAIDSAAEKQMI
                     REGSAKLCSIFDINDEAIATGHTEKMQFVVMLGGNEDSADYVIDCQTPNHAQNPLSAG
                     STQDSLNARIRSIYAQYHVELYMIYISALDVIIHTPLPPEITAANLFGRKLYDEQTNM
                     AELRSLHDNITNAIISNTIAGHHRDCVIHSCADYCGAFFKNAQTGCFTFSMNYAFRAP
                     SSTFYDIDGLRTNFSKRLNSNDTWLYSTGEVKAFAVTDAFRESAEVFSQRANILTTYT
                     SAGLNSILSGFAPGIDYESLSTEERRHIFSVYAGYLMLGDGPLGAHYEDYYTTMVVQY
                     TPPDQVLDLFTLLSRAGSLNNNANYQGEKNTKALIRRLIDRINDAPLGAPNYTRFMLA
                     LTDLLKTNDGIFSSLLPQNDEQWKERTFYWDDTYLFSCPPIGTHRYSVSIDDFGTMHV
                     RPGMVTAIRTDYNNAAATPNHTPVWNDSLEEEEIDPFAVVIFKNRSNYGMLEHAGAQR
                     NGVFFAPAIFLQYAADKTFNANALTMVAVSLDVATLTLGPGAVLTAMEAEDLGLAAFE
                     ALQSLGSGGNLAANALNDPSVTEVVSTYNEIIAAWGLSNLATHLGTFTVDFISGARAG
                     SVRPIPASLATEFESEYNTAKTYLASNDPNAQQLQKMEEYLEEKVGVVNSTPAGNFNF
                     QYLSLLNGFSDNLDGLLAMEGIDRNTFLVMEGKSAADLTPNELAMMNRIRARIPKPDA
                     NTLMQKAVPKSFFEGYLSGQYSSVSGYITSASDAKQFKTFEDIYYGLRLDYNGTRYSL
                     TDEYCGVIRFKASNIEEAYVPRSPANGGTTMEAPPFTGHGFTSGSHGRLGAPEWKMDV
                     PAILKDGAELWQIDANGDETLKAIYDSEIHKFVPVN"
     gene            12999..13433
                     /locus_tag="VFU15_12310"
     CDS             12999..13433
                     /locus_tag="VFU15_12310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718615.1"
                     /translation="MRNFEIALYCDQEFEITESNNEHYKLIYHGSISPGFGFYPSPKW
                     PNIFVRQINKSDIKSGYQASTFANYKGFKFHVDRYENEKYRLTTGDEKVYAQLHLNMI
                     DRGWYEIWVDKSEIEKMWEERKPSGYGFPMPEGLKKTQELNP"
     gene            13478..14254
                     /locus_tag="VFU15_12315"
     CDS             13478..14254
                     /locus_tag="VFU15_12315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEU4718616.1"
                     /translation="MKKRSVNWTREELILALNLYLKLPFGKLHSRNKAIIRLSQLIGR
                     SPNAVAMRLNNFASIDPFHQSRGIKGLTGGKKQVQPVWDEFIHHKENLLFESERILAE
                     KEGMSIEKKFSQDLVDLENIKGETKLREVKTRVNQSVFRQIVVANYSGKCAISGINIP
                     DFLVASHIIPWSKNEKERLNPANGICFSVLYDRAFDKGYIGLDNNYSVLVSTDLRQKS
                     KEPYYRSFFNELSGMKITLPQKYMPERDFLQYHRDVIFKG"
     gene            complement(14271..14660)
                     /locus_tag="VFU15_12320"
     CDS             complement(14271..14660)
                     /locus_tag="VFU15_12320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718617.1"
                     /translation="MVLLFVGFFEEEIGNIPWCMQEPFSIEQVAADDRRNFFYASVLW
                     VRKNNRTVVFVIGSIAIEIIEGKKWSHANRNYGLFTSVKVNMQRKQQWIIAPIPVRTL
                     QYGHVVGVDGMQKIWAGMLFSKMPGRG"
     gene            14788..15276
                     /locus_tag="VFU15_12325"
     CDS             14788..15276
                     /locus_tag="VFU15_12325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718618.1"
                     /translation="MRAFLFFLLFPFALCAQTKSDTLYVLPLKDSLYFRESNTFGMRS
                     DTAVLEQKEDYDAPMSIDEEYAHELFIYLDGVKSMHAGQTFDVQKDTFYVQVAYFFDS
                     VFDIENPGGPVSGTVKIISISNSEIVLGFNLRFRNFNQAWADSAYVVYRGQRAFSQQK
                     QK"
     gene            15347..17440
                     /locus_tag="VFU15_12330"
     CDS             15347..17440
                     /locus_tag="VFU15_12330"
                     /inference="COORDINATES: protein motif:HMM:NF020337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HEU4718619.1"
                     /translation="MKKLYFLSAFLLSSFLHAQTYNGSTGPVNDVSVNDFTVNVSGLS
                     PATIDTANFGLVSVTINLTHTWDADLDIQLISPDGTAALLSSGNGGSGDNYTGTVFIQ
                     GASQNIVTGSAPFTGSFVPQGQMGLVNNGQNGNGTWTLRVTDMASGDSGNMLGWSITF
                     GNTPATYFAFHDSNLPIVVINTNNQTIVDNPKIMADMGIIYNGPGNRNHMTDPHNNYD
                     GKIGIEIRGSSSQMFPKKSYGFETWDVNGNQIDSSLLGMPKENDWILCANYSDKSMLN
                     NTLTYHLASEMGRYAVRHQHVELVINGEYMGVYVLMEKIKRDANRVDISKLKPTDISG
                     DQLTGGYIIKIDKSTGNGGGGWISPFAPDTAPNGQTIFFQYEYPSDVNIVPQQEAYIQ
                     AYVDSFETALAGPNFADTATGYAHYIDVNSFVDYFLLNEMSRNVDGYRLSTFLYKNKY
                     SKGGKLVIGPVWDYDIAWGNADYCSGNDSTGWAYQFGNVCSGDNWQVPFWWDRLMQDS
                     VFRDRVKCRWAELSQTVLSVPSLHQYCDSMATYLNESQARNFIAWPILGTYVWPNPSP
                     VPTTYQGEIDELENWIDARWTWLDNNMPGTMAGCNLASVPQNTTANDLPPAYPNPFSD
                     AIHLSIYLPHAMPVQMELVNALGQTVQPVQTVQHTGGTQTITFTPDQDLPPGIYLLRI
                     TAGNVTWTQQLSRAE"
     gene            complement(17443..>17654)
                     /locus_tag="VFU15_12335"
     CDS             complement(17443..>17654)
                     /locus_tag="VFU15_12335"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEU4718620.1"
                     /translation="PADGSFYVRPAHAIDLLEVTDMTGRIVFAGKDYPAGTPAEITGL
                     ADGMYIVRAFSGGVISTQKVSVKSR"
BASE COUNT         4379 a         5002 c         4407 g         3866 t
ORIGIN      
        1 aagccagcag gtgaatatca cacaaccttc accgctgctt gctgtcgttt cttccactac
       61 atctgcatgc ggcatctgca acggaacggc atctgttgcc gtaagcgggg gaacttcacc
      121 atacgcgtat ttctggacgc cgtcgaacca gaccaacccg acagccacga atctctgcgt
      181 aggaagctat accgtcaccg ttactgatgc ggccggttgt acaaccactg ccattgccac
      241 gatcaaccag gtggtgaaca ttatcgtgac gagttccgca tcgagtctca gctgctcggg
      301 cgcctgcgac ggcgtcgctt ccgccaatgc ttcgggggga actgtgcctt attcctacag
      361 ctgggcgcct aacgggcaaa ccacgcaaac aatcctgaat caatgcgccg gaagctatac
      421 agtcaccgca acggatgctg cgggttgttt caacacagcg actgtcacat tcatcgatcc
      481 tcccccgatc gttatcacga tgacaaacac caatgcttcc tgcaacggtg tgtgcgatgg
      541 aacggccaca gcgaatgctt ccggaggaac gggcgcatac acttattcct gggcaccggg
      601 aggacagacc acgcaaacag ccacgggact ttgtgctgga acatacacgg ttactgtaca
      661 cgatgcgaat aattgcgtca gcacggaaac agttacgata acggaaccaa cactcatcac
      721 ggcaaacgcg gtaagcaacc cggccaactg cacgctgtgc gatggaagca tcaacaccgc
      781 agcatcggga ggaacaggtc cgtatactta ttcatggtcg cccggcgcac aaacgacacc
      841 gtcgatttcc aacctgtgtc ccggaattta ttccgtaacg atcaccgata atacgggatg
      901 ctcggttgtt attcctattt ccgtcagcaa catcaatggc cccaccgtcc aggcgacatc
      961 aaccgatgcc acctgcaacg gaacctgcga cggaacggga aatgcaaacg taaccggcgg
     1021 cgcttctcca ttcacctacg actggtcgcc gggtaatccc accggggacg gaacaccgaa
     1081 tgcaagcgga ctttgcgcag gatcctattt ctgccaggtg acagataacg tcggctgcat
     1141 aacctttgcg aacatcacca tcacgcagcc cacacctgtt tccgcgaata catcgcagac
     1201 caatatttcc tgcaacgggc tgacggacgg aagtctcagc gcaaccggaa ccgggggaac
     1261 cgggccttac acctacaact ggcttcccgg cggacaaaac acgcctgcga tatcgggtct
     1321 tggcgtcggc ggttataccg tgaatattac cgatgccaac ggatgcactt tttcgcagtc
     1381 gttcagcatt accgaacccg gaccgatatc cgcatcgatc gcctccacga atgtgcagtg
     1441 cttcggtgcg tgcaatggct ccgcgaccgt taccgttgtc ggtggctcgt ctccgtatgc
     1501 ttacagctgg tcgtccggac agggaacttc catcaccgcg aatctctgcc cgggatcata
     1561 cacagttgac gtgaccgatg taaacggctg caccacgaat caatccgtaa cgatcaccca
     1621 accgacacaa ctgtcgactt cgatcagctc gacgaacgtt acgtgcaacg cattatgcaa
     1681 tggtaccgct acagtcagcg cgagcggagg cgttggcgga tacacgtaca cgtggtcgcc
     1741 gaacggagga accacaccga ccgcatccgg tctttgcgcc ggtgcgtatt ccgtgatcac
     1801 caatgacagc aacgggtgcg cgtctgttca atccgtcacc atacaggaac ccccggccat
     1861 tgttctttcc gcgagcagcg gaccgctgag ctgcaacgga tcctgcaacg gtgtggcgag
     1921 tgttgttgct tccggcggaa ctcccggcta catttacaac tggtcaccgg gtggtcagac
     1981 aagcgcttcc gcaacttcac tctgcgcggg aatttatact gtgaacgtta cggatgccag
     2041 cggatgttcg caaaccgcaa cgactacggt tgcacaaccg gcggtcctcc aggccaacac
     2101 ttcgttcaca agtccgagct gcaataatgg aactaacgga actgcttccg ccaatcctgt
     2161 cggaggaacc ggtccgtaca gctatctctg ggcaccgggc ggacaaacaa catccacgat
     2221 caccggcctc agcgtcggaa cctacacggt aaccgttcat gatgccaatg gttgccagga
     2281 tgttcagacc ataaacgtaa ctgctgtttc accgatcgcg cttactgtcg ccagcgctcc
     2341 tgcttcctgc ggaaactgcg acgggacttt atctgtcact ccttcgggcg gcgcctcacc
     2401 gtatacctac aactggtcga acgcactgcc gaaccaaccg aaccagacga acctgtgcgc
     2461 aggaatttat tccgtgaccg taacggatgc ttcgggctgc gccaattcgt ttacgcttac
     2521 gatgaacaat tccggcggac ctaccggcga aacagttgtt tccaatgatg cgagctgtcc
     2581 cgcatcctgt gacggaagcg caacagttac accgatcggg ggagcagcac cgtatactta
     2641 cgtgtggaac ccgggcggac aaaccacgaa ctcgctcacg aacatgtgtg cgggaagtta
     2701 tttcctccag gtgaccgaca ccaacggatg catccgtttc tcgcctgtaa cgatcaatgc
     2761 tccgcaaccg atcaatgcaa atccgttcgt ttcaaacgca acctgcaccg gtatctgcga
     2821 cggatcgatc tcgctaagcc cgaccggagg aacaggtcca tacagctaca gctggtcgcc
     2881 cggcctccag acgacagcat ccatcacaaa ccagtgcatc ggcacctaca ccgcgacgat
     2941 cactgacgcc aacggatgta cgaccacttc atcttccact atcaatccgt ggaatacgct
     3001 tgccgctgtg gcgacttcat cgaacaccac ctgcagcgga agctgtaacg gaacagcgac
     3061 tgccacggtt accacgggaa gcgggccgtt cacctacaac tggtcggatc cgctcgggca
     3121 gaccaccgcg accgccacgg gtctttgccc gggaagctat acggttgtcg tcgccgacgg
     3181 tgcaggatgc aacacgacgc tgaacgtttc actgaacagc ccgacaccga tcacgatcaa
     3241 tcctgcggtc accccgacga catgcggatc gtgcaatggt tccgtcaccc tcaatacaag
     3301 cggaggcatc gcaccctaca ccttccagtg gtcgaacggt tccaccacgt cgacgttgaa
     3361 cggactctgt gcaggcgttt attccgtcat catccgtgac gccagcggct gcaccgccac
     3421 atacagtgtg aatatttcag caagcggcgg acccaccgcg gcaaatgcaa cagtgacgaa
     3481 cgctacctgc aacggaacct gcaacggctc cgcatcgctt gcaccgacag gaggaacacc
     3541 accgtattcg tacctgtggg tttccggcgg acaaacaact tcttcggtga acaacctttg
     3601 cgccgggctt tatttcttcc aggtgcggga ttctgcgggc tgtgctctta cagacaccgt
     3661 gcgtatcact gagccgccgc cgatcgtccc gaaccagacc attgtcaaca cgacgtgcgg
     3721 aatatgcctc ggctcgatct cgcttaaccc ttcgggagga agcgctccgt atacgtacaa
     3781 ctgggctccg ctcgggcaaa caacatccaa catcaccctg ctttgtgcag gcttgtacac
     3841 ggtaacgata accgatgcag cgggttgttc tataacacaa cccttcccgg taagcaattc
     3901 caactccacg ttagtcctgt caacttcagt aagcggcgcg ctgtgcaatg attcctgcaa
     3961 cggcatggca agcgtcagcg cttccggcgg atccgcccct tacacataca gctggagcaa
     4021 cggatccacc aatgattcca tcaccttcgt ctgcgctgga acttatctcg tgcaggcaac
     4081 ggatgccggc ggatgtgttt ccaccgcttc ggtcaatatt accgaacctt cttcactggc
     4141 cggctcctac ctgttcgtgc aggatgagct gtgcacgggc gtatgcaacg gattcatcac
     4201 cgccattcca tcgggaggaa cattgcccta cacatataac tggagcgatg cacagaccac
     4261 gcaaaccgca agcaaccttt gcgccggatc gtacaccgtc gtcatttcgg atgcgaacgg
     4321 ctgcagcgtc acgcaaaacg attcgatcat ttcaccggtt gtgcttgttc ccggtgtacc
     4381 gctgatcacc gatgcgacgt gcaataattc cgcggacggc gctatttcgt acagtgtttc
     4441 cggcggatcg ccgccgtaca cttattcgtg gacgggtccg aacggttaca cgtccgcgtc
     4501 acagaacatt tcaggaattc ttccgggcaa ttatacgctc accgtcacgg atgcgaacgg
     4561 atgctccata agcgacacgc tcgcggtcgg atcgaccatg gttgttcttg ccaacgccgg
     4621 gaatgacacc gtgttctgcc agtcgggatc tatcctgctc aacggaaccg gcagcacgaa
     4681 cgccgttact taccagtggg tgcagctgcc cggcatgaac gtggtcggca atgccgcaac
     4741 agcaacgatc actccaccgc caggcgcaac gacgtacgtg ctcatcgtga gcaacggcgc
     4801 ctgcacggac agcgattcga tcgtggtgaa ttcgcttcca ccgccgaacg cagacgcagg
     4861 cccggacatc gagattttca caggcatgaa cgccacaata ggcggctcgc cgacaggtcc
     4921 gccgggctcg acgttcgcat ggggaccgtc aacaggtctc ggcgacccga cgatctccaa
     4981 tcctgttgct gcaccgggaa caaccacgac atacacggtt gtggtcacgg acggaaacgg
     5041 ctgtaccgga agcgatacga tgacactgac cgtagttcct tcgattgagt tcccgaatgg
     5101 attttcgccg aacggcgacg gcatcaacga cgtctgggtg atcgacaaca tccagctttt
     5161 cccgcactgt catgtggaag tgtacaaccg ctggggtgaa atgctgtttg cttcggacgg
     5221 ttacaacaca ccatgggacg gacgttacca gggcaaggac cttcctgtcg gaacgtatta
     5281 ctacatcatc aagctgaatg atccgaattt cccggacgat tataccggcc ctatcaccat
     5341 catgcgataa aaacagaacg atgaaaaaag ctcttgtgtt tttatgctcc tgcatcgcga
     5401 tggtttcctt cgcgcagcag ctgccgcatt acagccagta tatgctgaac gattacgtga
     5461 tgaaccctgc catcggtggg aagaacaatt atttcatcgg catgtccgat aaccgttacc
     5521 agtggggcgg catcacggac gcgccgcgca catacgtgat gagcgtacac ggtccgctga
     5581 aatacgatca catgggaatg ggcggacaac tgttcaccga tattgtcggg ccgacacgac
     5641 gcaccggatt ttatctttcg tatgcgtacc acgcgccgct tacggagcac ctgaagcttt
     5701 ccttcggcat cagcgcgggc atcctgcagt ttatggtgga cgggcagaag atccacctgc
     5761 acgatcccgg tgataacgtg ctgacgaatt cgctgcagag cgtcctcact cccgatttcg
     5821 gcgcgggatt ttatctgtac agtgaacgtt tctgggtggg cgcatcctgc ctgcagatcc
     5881 agcagaacaa gctgaaattt ttcgattaca tgtcgaacac cacgagcgtg atgacccgtc
     5941 attattacgg catgctcggc taccgccagc ccatcggcaa tgatttcgtc ctggaacctt
     6001 ccgcgttgct gaaatacgtt tccccggcgc cattccagtt cgacgtaggc atgcggctta
     6061 tttacaagga aaaagtctgg ctgggcgggt cgtaccgtaa tctcgatgcg atcgcggctt
     6121 acatgggatt cgtggtgcgt gaaaatattc ttatcgggta ttcgtacgat tacaccacca
     6181 cgaacctcgg caactacaac aacggtacgc acgaagtgct gatcgggatc aaattcaaca
     6241 agaacggcgt gcagaaaccg aacgagtcgc agaccaagtt cgattgatta aaaaagtgat
     6301 cccgacccgc tgcgctttcg ggattaattc cgccaacgct tcgatcttca cttcgttcgt
     6361 tctcagtagg cgacgttttc agtgatgtga tcccgactcg ctgcgctttc gggattaatt
     6421 ccgccagcgc ttcgatcttc gcgctgctcg tgctcagcgg gcggcgtttt cagtgatgtg
     6481 atcccgactc gctgcgcttt cgggattaat tccgccagcg cttcgaactt cgcgctgctc
     6541 gttctcagcg ggcgtcatta aaaaaaaatc ccggatctct ccgggatttt ctctgctata
     6601 aaaaaagcct taagccagtt ttacattcac tgcgttgggt ccttttttac cctgctgaac
     6661 ttcaaaggta actttgtcgt tctcgcggat ctgctgggta agacccgtag catgaacgaa
     6721 aatctcttca ttcgtgccgt caactttgat gaatccaaat cccttggatt cattaaagaa
     6781 ttttactgtt ccattttgca ttgtgactga tgataatttg gttaataggt tccaaaggta
     6841 agtcaaacag acccagaaaa tgcaagtccg ggcgaatata ttccgctcca gggcccggtt
     6901 tttcaccttt tttatcacct gaaccggtat aattccggaa ctttccggcc tgaaaaacag
     6961 catcatgaaa acgattttca acgcttccga ttatgccggc atcgaagaac gcataaacag
     7021 gctgaccccc tcctccccgg cgctctgggg aaaaatgaac gttgcgcaga tgctcgccca
     7081 ttgttcggca acggtggaaa tggccttagg cgatgtcgtc ctgaaacgac ttttcatagg
     7141 gcggatcatc ggcccgctgg tgaagaaaaa gttcctcggg gaagagccgt tcaagcaggg
     7201 caacccgacc gccagggaat tcaccatcac cgacccgcgc gacttcgaaa aagaaaagca
     7261 gcgcctgctc gggctgatga aacgcctgca cgagggcggg gaagccggcg ccacgaagca
     7321 ccctcatggc tttttcggtc cgctcacacc ggcccaatgg gggcaaaccc agtggaaaca
     7381 ttttgatcat catttaaggc agtttggggt gtaggcttgc cagatttcga tatgccgcac
     7441 tgtatgcggc tactcaatct gacaagcggg ataggccaat ttacccggaa gaaattttct
     7501 gatcatcact tccccaacct gaacctgccg cacttgtcag attgagtagc tgcggccttg
     7561 cccagcgtat cgaaagctga caagcacgca aaccaatctc tgcagaagca tcctgatcat
     7621 cattttctca aacggaacct gccacacttg tttgcccagc gtaccgaaaa ctgacaagca
     7681 cgaaaaccaa tctctggaga agcatctgat catcacttcc ccaacctgaa cctgccgcac
     7741 ttgtcagatt tcgatacgcc gcactgcgtg cggctactca atctgacaag cgtgatagcc
     7801 caatttaccc ggaagaagca ttctgatcat cattttctca aacggaacct gccgcacttg
     7861 tttgcccagc gcaccgaaag ctgacaagca cgaaaaccaa tatcagcaga agcatcctga
     7921 tcatcacttc cccaacctga acctgccgca cttgtcaaat tgagtagctg cggccttgcc
     7981 cagcgcaccg aaagctgaca agcacgcaaa acattttcaa tcgcttattc ccgactaata
     8041 caattccacc gttgcacatt tgcctccttt atcattgacg gagcgcaatg cgtccctttt
     8101 gcttcacact gatcgctttc ttttttctct gcggctcttc ctccgcgcag aatttcccgg
     8161 tgcagctgaa cctccagctc gttccgcctt tctccggtta tctcaacgat tactgctcgc
     8221 cgggaaatga gaatttccgg gttctcgctt tattcaccga cttctcacat ccgtcgtatg
     8281 acgtgaagct gaaattcaag atcgagggac agggaatttc attgcagaac caggcctggt
     8341 attacgcagg tcccttcacg ctggaaccgg gtgtaccgaa aatgatcagc ggggatgatc
     8401 tctttccatt gatggatgaa gaccacctgg atttttccgg cattacacgc gcacaatacg
     8461 accagcggaa ggttctgccg gaaggctttt acacgatcac cgtcaccgcg tacgattatg
     8521 cgaatccgct gccagtgaaa gtgagcaacg acgccgtcac acaggcatgg atgctgctca
     8581 atgatccgcc cgcaacgaac ctgccggtgt gcggaacaac cgtgcaggaa gtttccccgc
     8641 agcagctcac cttttcctgg accccgatga acgtgacgtt gccctcatcg ctcgcgggga
     8701 ccgattacac gttcgagctg tgggaaatct atccctcaaa ttcttccgca ggaacgatcg
     8761 tcgcgacaac agcgccgctt tattccgctg ttacgcaaca gacgatgctc agctattcca
     8821 tcaccgaacc gccgctcgtt accgggcggg aatacgtatg gaggatacgt gcgcatgacg
     8881 cggacggaag agaactgttc cgcaacaacg gttattcgca gctctgcaca ttcacgtggg
     8941 gaaatgcgaa ttcactgctg ggaaacagcg cgaacctcac actcaccgcg caggcgctca
     9001 cgcaccgcca ggcgaaatgc tcgtgggaca gtctttccgt ttattcgtcc taccgcgttg
     9061 agttcaaaaa aacgggcagc gggaactggt ttcccgttac gacggatcat gcttcgctcc
     9121 gcatctgcga tctcgagccc gaaacaaatt acgaagcgca cgtgcagggc atttttcccg
     9181 acggaagtcc gggtccctgg agcaacgtcg cgacgtgggc gacacccgcg cagcagctgc
     9241 taaactgcgg agatatttcg cctgcgcctg cgcagcagaa ttttcagccg cttacacagg
     9301 cgaacgacgg aatgatctgg caggtgggac aatttgagat ggaagtcacg tcgctgagcg
     9361 cgtacacgaa tccgttcggg cattattccg gttacggaaa agtgatcaca cccttcggtg
     9421 gaataacgct caactgcgaa ttcaccagca tcaccatcgg cgatgatcac cagatgtacg
     9481 cgggcgaagt gaaagcggtc agcgaaggcg tatcgaactg gatgacgcaa ttcaatgcgg
     9541 gacaatttca ctacgatacg agctggttct acaacggaaa catcgacagc atctatgtaa
     9601 atgcgaacgg agagatcgtg ctactcgacg aaaacgggaa cacggttacg atcgggaaca
     9661 acacggacgg cggcgtgctc atcaccgacg cgaacggcaa ccagtggatc gtgaatcccg
     9721 acggagctgt cacgttcgtc accggggatt ttcttcttcc gctgacaaca gatacattga
     9781 ccgcgcatga aatgcagctg ctgaaaatgg cgatgaccat catccgcagc cagctttcgc
     9841 cgaatgatat ctccgccaga caaaatgtct ttgagacaag cgaaggaaac ctcggcaatt
     9901 acatttccac ccaacgccag tcgcttccct cttcgccggg caactcagga acgataacgt
     9961 ccgacagcgc cgcattcgtt tcctattacg aaaaaccgct gaacccgaac gatgcggggg
    10021 cacagctggg caacacttac aaaaccgcgc aggtcgatta ttattctgcg aaagtgcttg
    10081 ctgttctctc gcgcgcggac tgccccgatg acgaattgaa ttttatcgga caatacctta
    10141 ccgtgaatgg cgtgctgtac cacgaatacg tcacgcagca ggtggcgctg ggaaaaaccg
    10201 atcagcagat cgcggacgag gtggcggaga atggcgtgaa acaactggtg gtgatgacgc
    10261 tgcggaaaca gatgtcacgg tgataattac tgatgaacaa ccaatactta aaaatgaacg
    10321 cgaggaataa aagacacgga gcaggccgga aagcaatgat cgttttcgcg atcgtcctgc
    10381 aggctgtttt ttgcatcagg ggaaaagcgc tggcgatcga ttccgcggcg gaaaaacaaa
    10441 tgatccgtga aggaagcgcg aagctctgct ccattttcga tatcaacgac gaagccattg
    10501 ctacaggtca cacggagaaa atgcagttcg tcgtgatgct cggcggaaat gaggattccg
    10561 ccgattacgt catcgactgc cagacaccga accatgcgca gaacccgctt tcggccggca
    10621 gcacacagga ttcgctcaat gcgcgcatcc gcagcattta tgcgcaatac cacgtggagc
    10681 tgtacatgat ctacatcagc gcactcgacg tgatcatcca tacgccgctg ccgcccgaga
    10741 tcaccgctgc gaatcttttc ggacggaaac tatacgacga gcagaccaac atggcggaac
    10801 tgcgttcgct gcacgacaac atcacgaacg ccatcatcag caacaccatc gcgggtcacc
    10861 accgcgactg cgtcattcat tcctgcgcgg attactgcgg tgcgttcttc aaaaatgcgc
    10921 agaccggctg cttcaccttc agcatgaact acgcattccg tgcgccatca tccactttct
    10981 atgacatcga cgggctgcgc acgaattttt cgaaacggct gaactcgaac gacacgtggc
    11041 tctactccac cggtgaggta aaagcgttcg ccgttaccga tgcgttccgt gaatccgcag
    11101 aagtgttctc gcagcgggca aacattctta ccacgtacac atccgccggg ctcaacagca
    11161 tcctttccgg cttcgcgccg ggtatcgatt acgaaagcct ttctacggaa gaacgccgtc
    11221 acatcttttc cgtttacgcc ggttacctga tgctcggcga cggtccgctc ggcgcgcatt
    11281 acgaagatta ttacacgacg atggtggtgc aatacacgcc gcccgaccag gtgctcgatc
    11341 ttttcacgct tctttcgcgg gcgggatcgc tcaacaacaa cgcaaattac cagggcgaga
    11401 aaaatacgaa agcgctcatc cgccgcctga tcgaccggat caacgacgcg ccgctcggtg
    11461 cgccgaatta cacgcgcttc atgctcgccc tgaccgacct gctcaaaacg aacgacggca
    11521 ttttttcttc actgctgccg cagaacgatg agcaatggaa agagcgcaca ttttactggg
    11581 acgacacgta cctcttctcc tgtccgccca tcggcacgca ccgctattcc gtttccatcg
    11641 atgatttcgg cacgatgcac gtccggccgg gaatggtaac cgccatcagg accgactaca
    11701 acaatgccgc agccacgccg aatcacacgc ctgtgtggaa cgattcgctc gaagaagaag
    11761 agatcgatcc gttcgcggtg gtgatcttca agaaccgcag caactacggc atgctcgaac
    11821 atgcaggcgc gcagcgcaac ggtgtgttct tcgctcccgc gatctttttg cagtacgcgg
    11881 ccgacaaaac attcaacgca aatgcgctga cgatggtggc tgtttcgctc gacgtcgcca
    11941 cgctcacgct gggtccgggc gccgtgctga ccgcgatgga agccgaggac ctgggtcttg
    12001 ccgcgttcga ggcattgcag tcgctcggtt ccggcggaaa tctcgcggcg aacgcgctca
    12061 acgatccctc ggtaacagaa gtggtgagca cctacaacga gatcatcgcc gcgtggggac
    12121 tcagcaatct cgccacgcac ctcggcacct tcacggtcga cttcatctcc ggcgcgcggg
    12181 caggttccgt gcgacccatt ccagcctcgc tcgccacgga attcgaaagc gaatacaaca
    12241 ccgccaaaac ctacctcgcc tccaacgatc cgaatgcgca acagctgcag aagatggagg
    12301 aatacctgga ggagaaggtg ggggtggtga attctacgcc agcgggaaat tttaactttc
    12361 aatacctttc cttactcaac ggattttctg acaacctgga tggccttttg gctatggaag
    12421 gaatagaccg caacacgttt ttggttatgg aaggaaaatc ggccgcagat ctaactccga
    12481 acgaattggc aatgatgaat agaattcgcg caaggattcc gaagcctgat gcaaatacgt
    12541 tgatgcagaa ggcggttccg aaatccttct tcgaaggtta cctttcaggt cagtattcat
    12601 ctgtgagcgg ttacattaca agcgcatcag atgctaagca gtttaaaact tttgaagata
    12661 tttactatgg tttacgattg gactataacg gcacacgtta ttcattgaca gatgaatact
    12721 gtggtgtcat ccggttcaaa gcttcgaata ttgaggaagc atatgtacct cgaagtccag
    12781 ccaatggtgg aaccacaatg gaagctccac cttttacagg tcacggattt acgtctggaa
    12841 gccatggccg gttgggggct ccggagtgga aaatggatgt acctgcaatt ttaaaagatg
    12901 gtgccgaact ttggcaaatt gatgcaaatg gagacgaaac cttaaaagcg atttatgatt
    12961 ccgagattca taaattcgta ccggttaatt gaatcaatat gagaaatttc gaaatagcct
    13021 tgtactgtga tcaagaattc gaaataacgg aatcaaataa tgaacactat aaattgattt
    13081 accatggctc aatttctcct ggttttggat tttacccatc gccgaaatgg ccaaacattt
    13141 ttgttaggca aataaataaa tctgatataa aatcgggcta tcaggcttca acctttgcta
    13201 attataaagg ttttaaattt catgtcgatc gatacgaaaa tgaaaaatac aggttaacaa
    13261 cgggagatga aaaagtttat gctcaactgc acttaaatat gattgatcgc ggctggtatg
    13321 aaatatgggt cgacaaatct gaaattgaaa aaatgtggga agagagaaaa ccttctggtt
    13381 atggatttcc gatgccggaa gggttgaaaa aaacacaaga attaaatccc taacggtatt
    13441 cgcttttcat ttatttccaa cgaaatttga ttccccaatg aaaaaacgtt ctgtcaactg
    13501 gaccagggag gaacttatac tcgcactaaa tctttattta aaactcccct ttggaaaact
    13561 tcattcccgc aacaaggcaa ttatcagatt atcgcaatta ataggtcgct cgccaaacgc
    13621 agtggccatg cggcttaaca atttcgcaag catagaccct tttcaccaaa gccggggcat
    13681 caaaggactt accggcggaa agaaacaagt tcaacccgta tgggatgaat ttattcatca
    13741 taaggaaaac ctcctctttg aaagtgaacg aatacttgct gaaaaggaag gcatgtcgat
    13801 tgaaaagaag ttttcgcagg atctcgtcga tcttgaaaat atcaaaggag aaaccaaatt
    13861 acgtgaagtc aaaactcgcg taaatcaaag tgtatttcga caaatagtgg ttgctaatta
    13921 ttccgggaaa tgcgccataa gcggaattaa tattcccgat ttccttgttg ccagccatat
    13981 tatcccctgg tcgaaaaacg aaaaagagcg attgaatcct gccaacggga tttgtttctc
    14041 ggttctttat gatcgggctt ttgacaaagg atatatcgga ttagacaaca actattctgt
    14101 tttagtttca accgaccttc gtcaaaaaag caaagaacct tattacaggt cattctttaa
    14161 cgagttatcc ggaatgaaaa taacacttcc ccaaaagtat atgccggaaa gagattttct
    14221 ccaataccac agggacgtaa tttttaaagg ttaatttttc tcttgctatt ttatcctctt
    14281 cctggcatct ttgaaaacaa catgcccgcc caaattttct gcataccgtc cacgccaacc
    14341 acgtgtccgt attgtagcgt tcgcacgggt atgggcgcga tgatccactg ctgcttacgc
    14401 tgcatgttta cttttaccga ggtaaataaa ccataattcc tgtttgcatg actccatttc
    14461 tttccttcaa ttatctctat cgcgatgctt ccaattacaa aaaccacggt tctgttgttt
    14521 tttcgaaccc aaagcaccga agcgtagaag aagttacgtc gatcatccgc agcaacctgc
    14581 tcgatggaga atggttcctg catgcaccat ggaatattcc cgatctcttc ttcgaagaaa
    14641 ccaacgaaga gcaagaccat ccattgcacg aattcgtctc cgttacacct tgcgaaaacc
    14701 cgagaacgga ggtgggcagc atcgaagatt ttctctttct cctcccaaca tcccaccact
    14761 ctccggcata actccgtaaa caaccccatg cgcgcgtttc tctttttcct gctcttcccg
    14821 ttcgcgctgt gcgcgcaaac gaaaagcgat acgttgtacg tgctcccgct gaaggattcc
    14881 ctgtatttca gggagagcaa tactttcggc atgcgcagtg atacggcggt gctggaacag
    14941 aaggaagact atgacgcgcc gatgagcatt gatgaagaat acgcgcacga gctgttcatt
    15001 tacctggacg gcgtgaaaag catgcacgcg ggacaaacat tcgatgtgca gaaagatacc
    15061 ttttacgtgc aggtggccta tttcttcgac tccgtttttg atatagaaaa tcccggcggg
    15121 ccggtcagcg gaacggtgaa gatcatttcc atcagcaact ctgagattgt cctcggcttc
    15181 aacctccggt tcaggaattt caaccaggcc tgggcggatt ccgcatacgt cgtgtaccgc
    15241 ggacagcggg cattctcgca gcaaaagcag aagtgaggcg tccgcacata acattcccca
    15301 aacattttct taacttcaca taggccgttc ctcaacgcgg cgctacatga aaaaacttta
    15361 cttcctttcc gcgttcctgc tctcttcttt tctgcatgca cagacgtaca acggttccac
    15421 cgggccggtc aacgacgttt ccgtgaatga ttttaccgtg aacgtttccg gactgagtcc
    15481 ggcgacgata gacacagcga acttcgggct cgtgtcggtg acgatcaacc tcacgcatac
    15541 gtgggacgcc gatctcgaca tacagctcat ctctcccgac ggaacggctg cgctgctcag
    15601 cagcggcaac ggcggatcgg gcgacaatta cacgggaacc gttttcatcc agggcgcatc
    15661 gcagaacatc gtcacgggaa gcgcgccgtt caccggcagc ttcgttccgc agggacagat
    15721 gggactcgtg aacaacgggc agaacgggaa cggcacgtgg acgctgcgag tcaccgacat
    15781 ggcctccggc gattcgggca acatgctcgg ctggagcatc acgttcggaa atacgcccgc
    15841 cacgtatttc gcttttcatg attcgaatct tcccatcgtc gtcatcaaca cgaacaacca
    15901 gacgatcgtc gacaacccga agatcatggc tgacatgggc atcatttaca acgggcccgg
    15961 caaccggaac cacatgacgg atccgcacaa caattacgac ggcaagatcg gcatcgagat
    16021 ccgcggatca agctcgcaga tgttcccgaa aaaatcgtac ggcttcgaaa cgtgggacgt
    16081 gaacgggaac cagatcgact cttccctgct gggtatgccg aaagagaatg actggatact
    16141 ctgcgccaat tattccgaca agtccatgct gaacaatacg ctgacgtatc acctcgcttc
    16201 ggaaatggga aggtacgccg tgcgtcacca gcacgtggag ctcgtgatca acggggagta
    16261 catgggcgtt tatgtgctga tggaaaagat caagcgggac gccaaccgcg tggatatttc
    16321 caagctgaaa ccgacggata tttccggcga ccagctcacc ggcggttaca tcatcaagat
    16381 cgacaagagc acgggcaacg gcggcggcgg atggatttcc ccgttcgcgc cggacaccgc
    16441 gcccaacggg cagacgatct ttttccagta cgaatacccg agcgacgtga acattgttcc
    16501 ccagcaggaa gcttacatac aggcgtacgt cgacagcttc gaaacggcgc tggcaggtcc
    16561 gaacttcgcg gacaccgcga ccggctatgc acattacatc gatgtgaatt cattcgtgga
    16621 ttatttcctg ctgaatgaaa tgagccgcaa cgtggatggt taccggctga gcacattttt
    16681 atacaaaaac aaatacagca aaggcggaaa gctcgtcatc ggaccggtgt gggattacga
    16741 catcgcgtgg ggaaacgcag attactgcag cggcaacgat tcgacgggat gggcgtacca
    16801 gttcgggaac gtgtgttccg gcgacaactg gcaggttccg ttctggtggg accgcctgat
    16861 gcaggattcc gttttccgcg acagggtgaa atgccgctgg gcggagctga gccagaccgt
    16921 gctcagtgtt ccctcgcttc accagtattg tgattcgatg gcgacgtacc tcaacgaaag
    16981 ccaggcgcgg aatttcatcg cgtggccgat actcggaacg tatgtgtggc ccaacccgtc
    17041 gccggttccc accacgtacc agggagagat cgacgagctg gaaaactgga tcgatgcgcg
    17101 atggacatgg ctggacaata acatgcccgg caccatggcc ggatgcaatc ttgcttccgt
    17161 tccgcaaaac acgacggcga atgatcttcc tcctgcttac ccgaacccgt tcagcgatgc
    17221 gatccatctt tccatttacc tgccgcatgc catgccggtg cagatggagc tcgtgaatgc
    17281 gctcgggcaa acggtgcagc cggttcaaac ggtacagcac acgggcggaa cgcagacgat
    17341 cacgttcacg ccggaccagg atcttccgcc gggaatttac ctgcttcgca tcactgcagg
    17401 aaatgtcacc tggacgcagc agctttcaag agcggaataa ccttaccggc tttttacaga
    17461 aactttctgc gtggaaatga cgccgcctga aaaagcgcgg acgatataca ttccgtcagc
    17521 gagcccggta atctcagcgg gtgtgccggc gggataatct tttcctgcaa aaacaattct
    17581 tcccgtcatg tcggtgactt cgagcaggtc gatcgcgtgt gccgggcgta cataaaatga
    17641 tccgtcggca ggat
//
LOCUS       DASTSF010000427         2059 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1414322,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000427 DASTSF010000000
VERSION     DASTSF010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1414322"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..692
                     /locus_tag="VFU15_12340"
     CDS             <1..692
                     /locus_tag="VFU15_12340"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HEU4718621.1"
                     /translation="FANGLLRTSSVAEGTVVTARSQFAGRGQRGASWLSGEGQNITCS
                     IILRPVFLGASRQFDLTRAVALGITDLLLSLVPKKEFSIKWPNDIYTGGKKICGILIE
                     NQLSGNSISASVVGIGLNVNQAVFPAGVPDAVSLCYVADRKFNVEEVLQQLFGFVEAR
                     YLQLRAGSTKELGDEYESRLYRKGVPAKYTDFKTVFTATLDHVTPGGRLVLKMESGET
                     REFGFRELGWL"
     gene            complement(720..1427)
                     /locus_tag="VFU15_12345"
     CDS             complement(720..1427)
                     /locus_tag="VFU15_12345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1361 domain-containing protein"
                     /protein_id="HEU4718622.1"
                     /translation="MRNSLLFADETKRRSFFLLVAFSLFCFSLTVARCIYTGRRMFLF
                     LDWNLFLAGLPWIVSTLLVLHPGKKNKGIFSWLAIITWLLFFPNAPYMITDLFHLRTA
                     TDSPKWFDLMLVFSFAWTGIIAGFLSLRDIEQLLHSRLRGKFVRAVIGIMLFASGFGI
                     YLGRYLRQNSWDMLSGSQNIFHEIAQRFLHPQVHPRTWGMTLLFGIFLNLAYWSVGLF
                     GNAKPAQKEISTGTVEQ"
     gene            complement(1523..1804)
                     /locus_tag="VFU15_12350"
     CDS             complement(1523..1804)
                     /locus_tag="VFU15_12350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HEU4718623.1"
                     /translation="MLRLVKMKFTSEGVHAFLHIFSQSRDLIAAAPGCLHLELRRDME
                     DPDVFFTVSHWKTETDLENYRNSELFARTWARTKVLFAAKPEAWSLAET"
     gene            complement(1829..>2059)
                     /locus_tag="VFU15_12355"
     CDS             complement(1829..>2059)
                     /locus_tag="VFU15_12355"
                     /inference="COORDINATES: protein motif:HMM:NF042701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM hydroxide adenosyltransferase"
                     /protein_id="HEU4718624.1"
                     /translation="NITRADFSRLAKGRSFTIQCIGHEIDQLNHAYGDVAPGEIAAFF
                     NSAGVLEIAVNAGNAASLLNIRLNTIVRVIFE"
BASE COUNT          509 a          503 c          602 g          445 t
ORIGIN      
        1 cattcgccaa cggactcctg cgcacatctt ccgtggcgga aggaacagtg gtgacggccc
       61 gcagccagtt cgccggtcgg gggcaacggg gtgcttcctg gctgagcggg gaaggacaga
      121 acatcacgtg cagcattatc ctccgccccg tttttctcgg ggcttcacgc cagttcgacc
      181 tgacgcgcgc cgtggcgctg ggcatcaccg acctgcttct ttcgctggtt ccgaaaaagg
      241 aattcagcat caaatggccg aatgacattt acacgggagg aaagaaaata tgcggtattc
      301 tcattgagaa ccagctgagc ggaaacagca tttccgcttc tgtggttggc atcgggctga
      361 atgtgaacca ggcggttttt cccgcaggcg tgccggatgc ggtttcgctt tgttatgtgg
      421 cggaccggaa attcaatgtc gaggaagttc ttcagcaact gttcgggttt gtggaagccc
      481 gttacctgca gctgcgtgcc gggagtacga aagagctcgg cgacgaatac gaaagccgtc
      541 tttaccggaa aggggttcct gcaaagtaca cggacttcaa aacggtcttc acggccacgc
      601 tcgatcatgt gacaccggga ggaaggctcg tgctgaaaat ggaaagcggg gaaacaagag
      661 agttcgggtt ccgggaactg ggatggttgt gaagggggat gaacgataga taattttcct
      721 cactgttcaa cggtcccggt gctgatctcc ttttgagcgg gtttcgcatt gccgaaaagt
      781 cccaccgacc agtaggcgag attcaggaaa atgccgaaca gcaaggtcat tccccatgta
      841 cggggatgga cctgggggtg aaggaaccgt tgcgcgatct cgtgaaaaat attctgcgag
      901 ccggagagca tatcccagct gttctgcctt agataacgcc cgagataaat gccgaacccg
      961 gacgcgaaaa gcattatccc gataactgcc cgaacaaatt ttccacgtaa ccgggaatgc
     1021 agcagttgct caatgtcgcg gagactgaga aaaccggcaa tgatccctgt ccaggcaaac
     1081 gagaacacga gcatgaggtc aaaccatttc ggtgagtccg ttgcggttct caaatgaaaa
     1141 agatcggtga tcatgtacgg cgcattcgga aaaaacagga gccaggttat tatggccagc
     1201 caggaaaata ttcccttgtt tttcttcccg ggatgcagaa cgagaagtgt gctcacgatc
     1261 catggaaggc ctgccaggaa gagattccag tcgaggaaca ggaacatacg ccggccggta
     1321 taaatgcacc gggccacagt tagcgaaaag cagaacagcg aaaacgcgac caacaggaag
     1381 aatgatcttc gtttggtttc gtcagcgaaa agcagggaat tgcgcatagc gtttttgttt
     1441 cgctaacgca atagcgggaa taatgttgta cacccttgtc aatattccct ggcgcgacgc
     1501 aattaaggat tcaaaattca gttcaggtct cggcaaggct ccacgcttcc ggttttgcag
     1561 cgaaaagaac tttcgtcctc gcccacgtgc gtgcaaacaa ttcgctgttg cggtaatttt
     1621 cgagatcggt ttctgttttc cagtgggaga cggtgaaaaa aacgtccggg tcttccatat
     1681 ccctgcgaag ctcgaggtga agacagcccg gcgcagcggc gataaggtcg cggctttggg
     1741 aaaagatgtg gaggaaggcg tgaacacctt cgctggtgaa tttcattttg acgaggcgga
     1801 gcataggcag gtttcaggga gattaaattc attcgaagat cacgcgcacg atcgtgttca
     1861 gccggatgtt gagaagcgat gcggcattcc cggcgttgac ggcgatctcg agtacgcccg
     1921 cagagttgaa aaaagcggcg atctcgcccg gcgccacatc accgtacgca tgattgagct
     1981 ggtcgatctc gtgaccgatg cactggatcg tgaagcttct tccttttgcc agccgggaaa
     2041 agtcggcgcg cgtgatgtt
//
LOCUS       DASTSF010000428         8693 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_124101,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000428 DASTSF010000000
VERSION     DASTSF010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 8693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8693
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124101"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..401
                     /locus_tag="VFU15_12360"
     CDS             <1..401
                     /locus_tag="VFU15_12360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013407983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GMP synthase (glutamine-hydrolyzing)"
                     /protein_id="HEU4718625.1"
                     /translation="DHNILHRHPFPGPGLGIRILGEVTEEKVRILQEADHIFIQGLKN
                     SNLYNEVWQAGVILLPVRSVGVMGDERTYENTVALRAVTSTDGMTADWCHLPYEFLGK
                     VSNEIINRVKGINRVVFDISSKPPATIEWE"
     gene            401..2038
                     /locus_tag="VFU15_12365"
     CDS             401..2038
                     /locus_tag="VFU15_12365"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEU4718626.1"
                     /translation="MGLFPHNDKKLFIITMLVMMIGFSFPLHAQNDGGVKKSAKTEIV
                     DGKKYYLHKVEKGQTLYAIAKAYDLTVNDIIVENPDAMNGIHPGQVLRIPFEKPKPVA
                     TAASSSDSTFVIKVQQGQTLYSIAHQYNTTEDAILKLNPDAKGGLKTGQDLRIPGKKK
                     EPVLPALVGNENPSQADTLYNGKKKDSYNIAFMLPLQLWNVDNINPGDPQPSIPQKAQ
                     ASVDFYEGALIAIDSMKRRGMNVHVYVYDTDDADSAKVQSILSKPEFSEMDMIIGPLS
                     PGPFYPVSEWAKAHNVPVISPVSPANRVLFKRPEALKALPGISTQMEGAADYLAAAHP
                     KDNIILFNSGNTKDNAAAGAFSDRMNQLLFPSGNDSIRSVKSFSAIGQNMKIDKLNIV
                     VIPSQNQAFVSDLLRQLNGLTDKYSIMVFGMSFWMSYDLDPEYLEKLQVHFPTAYYVD
                     YDSSASVQRFVKRYQAVYNGDPSMYAFAGYDVTMFFLNAMFTYGTDFYRKREALKGTG
                     LQQDFDFVRSDEESGFENNGVRIVGVKDYHLFLEKQQ"
     gene            2040..2930
                     /gene="hemF"
                     /locus_tag="VFU15_12370"
     CDS             2040..2930
                     /gene="hemF"
                     /locus_tag="VFU15_12370"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009196867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HEU4718627.1"
                     /translation="MALPAEAVSARFRELQDSICRALEDEDGKGKFHEDIWERPGGGG
                     GRTRIMRHGNVIEKGGVNFSAVHGELPENIRGALNVQQHKFFATGVSIVIHPESPMVP
                     IIHMNVRYFEMENGQFWFGGGIDLTPHYIDEKDAGWFHQRLKEVCDRHSNEYYDRFKK
                     WADDYFFIKHRNETRGIGGIFFDRLTGEKEKLFSFVTDVGELFAPVYTNFMRLHRDDP
                     YGEKEKSWQLLRRGRYVEFNLVYDKGTKFGLDTSGRTESILMSLPEKAGWEYDFVPVK
                     GSPEEKTTLALVKGMDWLNR"
     gene            3044..6517
                     /locus_tag="VFU15_12375"
     CDS             3044..6517
                     /locus_tag="VFU15_12375"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HEU4718628.1"
                     /translation="MKHRNTCFRNALQSAGILFFIAFFSVNSSAQSNAVTGNNSVYGD
                     SLDGFNVAYAYQKAAAKGLDHNETQLFVTRCEKQFIAHKYNIPQQKNTSLAAMRIPAT
                     VAQAPCTNMDFETGNFTGWTGFIGDNTVNSNGPLQNITNGFFSTTMDAAISDMNARHT
                     IMSIAGGTDPYGGFPVVAPGGQYSCRLGNNYANYQGEAIEQTFTVAAGNTSFTYQYAA
                     VLNDGGHSSGEQPYFRIEMFDQSGNLIPCSQYYVEAAGGNTPGFTVSPIDSYTYYKPW
                     TTVNVDLTNYMASSVTIRFTAAGCIWGGHFGYAYVDASCLPYSISLSDSLCQGGNITL
                     TAPAGAASYQWSTGSTSQSINVNTAGSYSVTMTSVTGCQTTLTRNVAMYPPPTASFTA
                     AAPSCASNFSFTNNSSISSGTLSYSWDFGDPSTTSDTSNVQSPNYTYTNTGTYTVTLT
                     VTSNNGCTDTYTQTVTAGSGSNAAFSATSVCVGTATQFTDQSVNAVSWDWDFGDPGSG
                     PNNTSSLQNPSHTFSAPGTYNVTLTAGTSPCNSVITQTVTVNPIPTASFVYNQVCGQQ
                     LVNFTSTSTVANPSTITSTSWNFGDPASGGNNTSTLTNPSHTFTAQGTYTVTLTVTTG
                     SGCPQTTTMVISVGVPPTSAFVASTVCTNSPMQFTNQSVNAGSYSWNFGDPSTIADTS
                     NATNPAYTYTNPGTYTVTLIANPGPCSDTSSLVVNVAPGPVVQFTAPPVCEANPTSFT
                     DQSNISSGSITGWSWDFGVAASSSDTSNVQNPNFTYPASGTYTVSLTCTSNNGCVTTN
                     TMAVTVNGLPTANFSSNVVCAGQPTSFTDLSNGNPGSVTQWTWNFGDGSPTASSQNPT
                     HTYTNDTTYNVTLIVSNAGGCSDTVTLQAVTASMPNVQFMADTLHGCPVLCVNFTDQT
                     TIASGSLTGWVWDFGDNSGQDFTQNPSHCYATPGTYTVTLTASSGNGCASTLVIPDMI
                     TVYPVPVADFSANPQHTTVLNADINFFDLSQGANYWQWDFGDPTTTSDTSSMQDPFYA
                     YTTEYGSNYDVHLQVANQYGCMDDTTIEVIIDPEFTFYIPNAFTPNGDGINDVFYGTG
                     IGITSYTMYVFDRWGNLIFTANDINQSWDGTVQGKSGQLCQEDVYVWKVVLTDVFDKK
                     HKYIGHVSLIR"
     gene            6654..7205
                     /locus_tag="VFU15_12380"
     CDS             6654..7205
                     /locus_tag="VFU15_12380"
                     /inference="COORDINATES: protein motif:HMM:NF019284.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718629.1"
                     /translation="MKHILTLAIFLCASSLAFAQFNHVETGPNGNKIAEGQYNADPGI
                     NPGDSKDVIAQKMGAVHKIGSWKYWFDNGQLSAEEHYDNNGNVTGVWKTWYNDGKLAS
                     EIDYTTGKAVYYHPNGAKSEEGTVNQYQQRTGKWQGWFEDGKLNYIGSFDNAGRKDGV
                     WQYYDDQGNIVGTEHYSQGAMVN"
     gene            7290..7928
                     /gene="nth"
                     /locus_tag="VFU15_12385"
     CDS             7290..7928
                     /gene="nth"
                     /locus_tag="VFU15_12385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007652955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEU4718630.1"
                     /translation="MTKKERFEEVIRWFEKNMPEAQTELHYDNPFQLLVAVILSAQCT
                     DKRVNIVTPALFRRFPDAETMAASSPGEIFTFIKSISYPNNKAKHLHGMAKMLSEKYE
                     GKVPGSIEELVKLPGVGRKTANVIASVIFEQAAMAVDTHVFRVSARLGLTTNAKTPLQ
                     AEQQLVSYIPEEKLAIAHHWLILHGRYVCLARNPLCEKCVLTGFCLYYRKRK"
BASE COUNT         2181 a         2215 c         2201 g         2096 t
ORIGIN      
        1 tcgatcacaa cattcttcac cgccatcctt tccccggtcc cggcctcgga atacgcattc
       61 tcggcgaagt gacggaagaa aaggtacgca tattgcagga ggctgatcat atattcatcc
      121 agggtttgaa aaacagcaac ctgtataatg aagtctggca ggcaggtgtg atcctgcttc
      181 ctgtacgcag cgtgggcgtt atgggagatg aacgcacgta cgagaacacg gttgctctgc
      241 gcgccgtaac ttcgaccgac ggaatgacgg ccgactggtg tcatctcccg tatgaatttc
      301 tcgggaaagt atcgaacgag atcatcaacc gggtaaaagg aatcaacagg gtggtattcg
      361 atatcagctc gaagccgcct gcaaccatcg aatgggaata atgggtcttt ttccgcacaa
      421 cgataaaaag ttattcatca tcaccatgct cgtgatgatg atcggctttt cttttccgct
      481 ccacgcgcag aatgacggcg gagtgaaaaa gtccgcgaag acggagatcg ttgacggaaa
      541 aaaatactac ctccataaag tagaaaaagg gcagacgctt tacgcgatcg cgaaagcata
      601 cgatctgacg gtgaatgaca tcatcgtcga aaatcccgat gcgatgaacg gtatccatcc
      661 cgggcaggtg ttacgtatcc cgtttgaaaa accgaagcct gtagcaactg ccgcttcttc
      721 gtccgacagc acattcgtga taaaagttca gcagggacaa acactttaca gcatcgcgca
      781 ccagtataac acgacggaag atgcgatcct taagcttaat cctgacgcga agggtggcct
      841 caaaaccggc caggacctgc gcatcccggg aaagaaaaag gaacctgttc tgcccgcgct
      901 ggtcgggaat gaaaacccgt cacaggcgga tacgctctac aacgggaaaa agaaggactc
      961 gtacaacatt gcgttcatgc ttccgctcca gctctggaac gtggacaata tcaaccccgg
     1021 cgatccgcag ccgtctattc cccagaaggc gcaggcttcc gtcgattttt acgagggtgc
     1081 gctcatcgcc atcgattcga tgaaacgcag gggaatgaac gtgcacgtct atgtttacga
     1141 cacggacgac gccgattccg ccaaagtgca gtcgatcctg tcgaaacccg agttcagcga
     1201 aatggacatg atcatcggac cgctgagtcc cgggcctttc tatcccgttt ccgaatgggc
     1261 aaaagcgcac aatgtcccgg ttatttcccc ggtttcgcct gccaaccgcg tgctcttcaa
     1321 acgcccggaa gccctcaagg cattgcccgg aatttccacg cagatggaag gcgccgcaga
     1381 ttatctcgcg gcggcgcatc ccaaagacaa tatcatcctg ttcaacagcg ggaatacgaa
     1441 agacaatgcg gctgcgggag ctttcagcga ccggatgaat cagctgctgt tcccttcggg
     1501 caacgacagc atcaggtcgg tgaaaagttt ttccgccatc gggcagaaca tgaaaataga
     1561 caagctgaac attgtcgtca tcccttcgca gaaccaggct ttcgtttccg acctgctccg
     1621 ccagctgaac gggcttacgg acaaatattc catcatggtc ttcgggatgt cgttctggat
     1681 gagctatgac ctcgaccccg aatacctcga aaagctgcag gtgcattttc caacggctta
     1741 ttacgtcgat tatgattcgt ccgcaagcgt tcagcgtttc gtgaaacgtt accaggctgt
     1801 ttacaatgga gatccctcca tgtatgcatt cgcaggatat gatgtgacga tgtttttcct
     1861 caatgccatg ttcacgtacg gaacggattt ttaccggaag cgggaagcac tcaaaggtac
     1921 cgggctccag caggattttg attttgtccg cagcgacgag gaaagcggtt ttgaaaataa
     1981 cggcgtgcgc attgtgggcg tgaaggacta tcatcttttc ctcgaaaaac aacaatagca
     2041 tggctttacc ggcggaagct gtttcagcgc gtttcaggga attgcaggat tcgatctgcc
     2101 gggcgcttga agacgaggac ggcaagggga aattccacga agatatttgg gaacgtcccg
     2161 gtggaggcgg tggccgcacg cggatcatgc gtcacgggaa tgtgatcgaa aaaggcggtg
     2221 tgaatttttc cgctgtgcac ggggaattac ctgaaaacat aaggggtgcg ctcaatgttc
     2281 agcagcataa atttttcgcc accggtgttt ccattgtcat ccatccggaa agtccgatgg
     2341 ttcccatcat tcacatgaac gtccgttatt ttgaaatgga gaacgggcag ttctggtttg
     2401 gcggcgggat cgatcttaca ccgcattaca ttgacgaaaa ggatgccggg tggtttcacc
     2461 agcggctgaa ggaagtctgc gaccgtcaca gcaatgagta ttacgaccgg ttcaaaaaat
     2521 gggcggacga ttattttttt ataaaacaca ggaacgaaac acgcggtatc ggcgggatct
     2581 tttttgatcg tcttacgggc gaaaaggaaa agctgttttc atttgtcacg gatgtcggcg
     2641 aattgttcgc gccggtttat acgaatttca tgcgccttca ccgggatgat ccttacggcg
     2701 aaaaagaaaa gagctggcag ctgctccggc ggggaagata cgtggagttc aatctcgttt
     2761 atgataaggg aacaaaattc gggctcgata cttccggcag gaccgagtct atcctgatga
     2821 gcctgcccga aaaggccggg tgggaatatg attttgtacc ggtaaaagga tctcctgaag
     2881 aaaaaacaac cctcgcgctg gtgaagggca tggactggct gaatcgataa atcacctttt
     2941 ttcgtagata atcttccctt ttacgtccat ctattggttt tctcggcatt gcttattata
     3001 ttgcgcaggc ttttaggaag ttaactgacc ttaaccttac ctaatgaaac acagaaatac
     3061 ctgtttcagg aatgcgttgc aaagtgcagg cattctgttt tttattgctt ttttctccgt
     3121 aaactcctct gcacaatcga atgctgttac agggaataat tccgtttacg gcgattcact
     3181 ggatggattc aacgtggctt atgcttatca gaaggcggct gcaaagggac tcgatcacaa
     3241 tgaaacgcag ttgttcgtaa cgcggtgtga aaagcaattc atcgcgcaca aatacaatat
     3301 cccgcagcag aaaaatacga gtcttgccgc aatgcgtatt ccggcgaccg ttgcgcaggc
     3361 gccgtgcacc aacatggatt ttgaaaccgg caacttcacc ggctggaccg ggtttatcgg
     3421 tgacaatacc gtgaattcga acggacctct gcagaacatc accaatggtt ttttctctac
     3481 tacgatggat gcggcgatct cggatatgaa cgcaaggcat acgatcatgt ccattgccgg
     3541 cggaacggat ccttacggtg gttttcctgt tgttgctccc ggtggacaat attcctgccg
     3601 cctgggtaat aattacgcga attaccaggg agaagcgatc gagcagacat ttacagttgc
     3661 agccgggaat acatctttca cataccagta tgccgctgtc ctgaatgatg gcggccactc
     3721 ttccggtgag caaccgtatt tccggattga aatgttcgat cagtcgggaa accttattcc
     3781 ttgctcacag tattatgtgg aagctgcagg aggaaatact cccgggttta ctgtaagtcc
     3841 catcgattcc tacacctatt acaaaccgtg gacaacggtg aacgtcgacc ttacgaatta
     3901 tatggcgtcc agcgttacca ttcgcttcac agctgccggt tgtatctggg gaggccattt
     3961 cggttacgca tacgttgacg cttcctgtct cccttatagc atttcattga gcgattccct
     4021 gtgccagggt ggaaatataa ccttgaccgc gccggccggt gccgccagct accaatggag
     4081 taccggctcc acatcgcaat cgataaatgt caatacggcc ggaagctatt ccgtaacgat
     4141 gacatccgtt accggttgcc agaccacgct tacgcggaac gtagccatgt atcccccgcc
     4201 gacggcctca tttacggctg ccgcacccag ctgcgcttcc aacttcagct tcaccaacaa
     4261 cagttcgatt tccagcggaa cgctatccta ttcatgggat ttcggagacc caagcacgac
     4321 aagcgataca tcgaatgttc aaagtccgaa ttacacgtat accaacaccg gaacttatac
     4381 tgttaccctg actgtgacaa gtaataatgg atgtaccgac acgtacacgc aaaccgtcac
     4441 tgcgggcagc ggcagcaatg ccgcattcag tgcgacttcg gtctgtgtgg gtacggctac
     4501 ccagtttacg gaccagtccg taaatgcggt cagctgggac tgggatttcg gagatccggg
     4561 ttcagggccc aataacacct cttcgctgca gaatccaagt catacgttct cagcgccggg
     4621 tacatacaat gttacattaa cagccggtac gagtccgtgc aactcggtga tcacacaaac
     4681 cgttacggtt aacccgatac caacggcgtc cttcgtgtat aaccaggtat gcggccagca
     4741 actcgtgaat tttacgagca catctaccgt ggcgaaccca tccacgatta ccagcacaag
     4801 ctggaatttt ggtgatcctg ccagcggcgg caacaatact tccacgctga ccaatccatc
     4861 gcacacattc accgcgcagg gaacttatac ggttacactc acggtaacca ccggcagcgg
     4921 ctgtccgcag accactacga tggtcatcag cgttggtgtg ccgcccacat cggcgtttgt
     4981 tgcatcgaca gtttgcacaa actccccgat gcagttcacg aaccagtctg taaacgcagg
     5041 aagctattcg tggaacttcg gcgatccgtc aacgattgcg gatacgagta acgccacgaa
     5101 cccggcgtac acgtatacaa acccgggaac gtataccgta accctgatcg caaatccggg
     5161 tccgtgctca gacacctcct cgcttgtcgt gaatgtggct ccgggtccgg ttgttcagtt
     5221 tactgctccg ccggtctgcg aagcgaaccc gacttcattt acggatcagt ccaacatttc
     5281 ttccggaagc attacgggct ggtcatggga tttcggtgtc gccgcttctt cctctgatac
     5341 gtcgaacgtg cagaacccga actttactta tcctgccagc ggaacgtata ccgtttcgct
     5401 tacctgcact tcgaacaacg ggtgtgttac gaccaacaca atggccgtaa cagttaacgg
     5461 gttgccgacg gcaaacttca gctcgaatgt ggtctgcgcc ggtcagccga cttcatttac
     5521 ggatctttcc aacggaaacc ccggcagcgt gacgcagtgg acatggaatt tcggcgacgg
     5581 ctcaccgaca gcttcgtcgc agaatccgac ccatacgtat acaaatgata cgacgtacaa
     5641 tgtgacgctc atcgtttcaa atgcgggcgg atgttccgat acagtgacgc ttcaggcggt
     5701 tactgcttcc atgccgaatg ttcagtttat ggccgacacg ctgcatggct gtcccgttct
     5761 ttgcgtgaat ttcacggatc agacgaccat tgcctccggt tcgcttaccg gttgggtatg
     5821 ggatttcggt gataacagtg gtcaggactt tacacaaaat ccgagtcact gctacgcgac
     5881 gcccgggacg tacacggtga cgcttaccgc aagttccggt aacggttgtg cgagcacgct
     5941 ggtgatcccg gacatgatca ctgtctaccc ggtgcctgtt gcagatttct ctgcgaatcc
     6001 gcagcatacc actgttctca atgcggatat caatttcttc gacctttcgc agggcgccaa
     6061 ttactggcag tgggacttcg gtgatcctac gacaacatct gatacatcca gcatgcagga
     6121 tccgttctat gcctatacga ctgaatacgg ctccaactac gatgttcatc ttcaggtggc
     6181 gaaccaatat ggctgcatgg atgatacgac gatcgaagtc attatcgacc ccgaattcac
     6241 attctacatc ccgaatgcat tcacaccgaa cggcgatgga atcaatgacg tgttctacgg
     6301 aaccggcatc ggcatcactt catatacgat gtatgtgttc gatcgctggg gcaacctgat
     6361 attcactgct aacgacatta accagtcgtg ggacggaacc gtgcagggca agagcggaca
     6421 gctttgccag gaagatgtat acgtctggaa agtagtgctg acggatgtgt tcgataagaa
     6481 gcataaatat atcggccacg tttccctcat ccggtaacgg gcaaaggggc ggtgtaaacc
     6541 gccccttttt tatcggttta aatctctcgt tcatctgtgt tgatatatct tagggccggg
     6601 ttcttgcaaa agatcggttg attgattaag tttgtttaaa ttcattcggc aaaatgaaac
     6661 acatactgac cctcgctatt tttctctgtg cttcgtcatt ggcgtttgcg cagttcaacc
     6721 acgtggaaac cggccccaac ggtaataaaa tcgctgaagg gcaatataac gcagatcccg
     6781 gaataaatcc cggggacagc aaagatgtta ttgcccagaa aatgggtgct gtgcataaga
     6841 tcggctcctg gaaatactgg tttgataacg gccagctttc cgctgaagag cattatgaca
     6901 acaacggaaa tgtgacaggt gtatggaaga cctggtacaa tgacgggaaa ctggcttccg
     6961 agatcgatta tacaacaggc aaggccgttt attatcatcc caacggagct aaatcggaag
     7021 aaggaacagt gaaccagtac cagcagcgca ccggaaagtg gcagggctgg ttcgaggatg
     7081 gaaagctcaa ttacatcggt tcattcgata atgccggccg caaggatggc gtatggcagt
     7141 attatgatga ccagggaaac atcgtcggca ccgagcatta cagccagggc gcgatggtca
     7201 actgaaacct gaaaaaaaaa caggaacgct ttcttcggga agcgtttttt tttgctcttt
     7261 ttgaaacgaa aataaagtcg ggatacacca tgaccaaaaa ggaacggttt gaagaagtga
     7321 tccgctggtt tgaaaaaaac atgccggaag cgcagacgga attacattac gataatccct
     7381 tccagctgct tgttgcggtg atcctttcgg cccagtgtac agacaagcgg gtgaacatcg
     7441 tcacgccggc tctttttcgc cgctttccgg atgcagaaac gatggctgct tcttctcccg
     7501 gtgagatctt cacattcatc aaaagcatca gttacccgaa caacaaggcg aagcatcttc
     7561 acggaatggc aaaaatgctt tcggagaagt atgaaggaaa agttcccggc tccattgaag
     7621 aactggtgaa gctgcccggc gtcgggcgga aaacggccaa cgtgatcgct tccgttattt
     7681 ttgagcaagc tgcaatggcg gtggatacgc atgtcttccg ggtttccgca cggctcggtc
     7741 ttaccacgaa cgccaaaacc ccgctgcagg cggagcagca attggtgagc tatattccgg
     7801 aagagaagct tgccattgcg catcactggc tgatccttca cgggcgctat gtctgtctcg
     7861 ccaggaaccc gctgtgtgaa aaatgtgtgt tgaccggttt ttgcctctat tacaggaaaa
     7921 ggaaatgacg ggaaacctct cgggcagggc attttttcaa cgcgcgctat ttctgtaact
     7981 tttttttatc cggataatta tactgcataa cgatggaagg gttgttttcg tcggaaaatc
     8041 ggaagtttta ttaacaggcg ccggaaattt acttcaaaag ctaatttgtt tttaacacgt
     8101 ttgtcgtaac tttaagacgg caaacagagt attttatcag catgaaacac acgggacgaa
     8161 gtctttccag actattgctt tttgtctttt tccttattgc tgccggtcag gaagcgaaag
     8221 cctgtcatgg tgttgcgctt gttggatttt ctgcttcggt ttcagggggc aatgtggtcg
     8281 tcaacggaag ttccgatgcg gctacatgcg gatgcggtcc ttattacatg gaagtcgagc
     8341 tcgcctgttt ttcaactgca aactttacgg ggaatgcgcc tacctgcact gcgtcaacgt
     8401 ggaatactta tccctggtac cgttcccttc ttaatgttcc caattatacc gcggcagcgg
     8461 gttggcccga taactgcgtc gttgagccat acaattctgt cgtcataccc atctcttctt
     8521 tatgtccggg cacacagtat gtactgaggg cgcgagagag ggtatgcggt tccggaagcg
     8581 gcgggccatg gtcaagcaca ttcacgttta ctacgcccgg aacgcctcct agttttacgt
     8641 tgacagcgac agcaaacccg agcactattt gtcccggcac acctgtcacg ctt
//
LOCUS       DASTSF010000429         7074 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1911632,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000429 DASTSF010000000
VERSION     DASTSF010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 7074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7074
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1911632"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="VFU15_12390"
     CDS             <1..285
                     /locus_tag="VFU15_12390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718631.1"
                     /translation="WNRFSDYYDSGFFHRYHSQHLGDIQYKVFWYAFELAWQWQLTEK
                     LDFNFGFTPGIPLAMSFSAGLTYWPHRFEKKIEQPKVHLGRRKNPPVSAN"
     gene            384..605
                     /locus_tag="VFU15_12395"
     CDS             384..605
                     /locus_tag="VFU15_12395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718632.1"
                     /translation="MKTYIAKSMLSIFAVLLFTSVYAGDPVACPGQSHSSRTHNGTVA
                     HHPQQRKRSDYHPQRKLQTKGKSYRRHRR"
     gene            complement(837..2120)
                     /locus_tag="VFU15_12400"
     CDS             complement(837..2120)
                     /locus_tag="VFU15_12400"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014202102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex dihydrolipoamide
                     acetyltransferase"
                     /protein_id="HEU4718633.1"
                     /translation="MAEVVKMPKLSDTMTEGVVATWNKKVGDTVKRGDILAEIESDKA
                     TMEFESFYNGTLLYIGVEKGQAAPVDAILCIIGKPDEDVKAIIAAASEKKAETEKKEE
                     KKAEPVAEQKTVAPVAEKKQEAAKPVAKPVTVAVEENGDGRIKASPLAKKLAAEKGLD
                     LHGVSGTGEYGRVVKRDVESWHPGKTGMISFGMGAGVVNGKESYTEEPVSQMRKTIAR
                     RLAESKFSAPHFYLTMEIDMEQAVKSREAINAISPVKISFNDMVIRACATALRYNPKV
                     NSSWLGDKIRYNHHVHIGVAVAVEEGLLVPVVRFADQKSFAQIGAEVKEFAKKAKEKK
                     LQPSDWEGNTFTISNLGMFGIEEFTAIINPPDSCILAVGGIKDVPVVKNGQVVPGKIM
                     KVTLSCDHRVVDGATGSAFLQSLKMMLENPVTILM"
     gene            complement(2414..3439)
                     /gene="pdhA"
                     /locus_tag="VFU15_12405"
     CDS             complement(2414..3439)
                     /gene="pdhA"
                     /locus_tag="VFU15_12405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007658325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HEU4718634.1"
                     /translation="MATKTKSSPEKQKAAFSREVYTQWYESMLLMRRFEEKCGELYIQ
                     QKIRGFCHLYIGQEALVAGAESAITKDDRVITAYRDHAHPIGRGLHPKFILAELLAKI
                     TGCSKGKGGSMHLFSKEHNFFGGHGIVGGQIPLGAGIAFADKYNGNNRVTLCYMGDGA
                     VRQGALHETFNMSMLWKLPVVFIIENNNYAMGTSVERTSNVHDLYKLGSSYNMPSYQV
                     DGMRCETVHEAIASAAEHARKGNGPTLLEMKTYRYRGHSMSDPAKYRSKEEVETYKKH
                     DAIETVIETMKANGWLDDKGVEAMEKKIEDIVNEAVKFAEESPFPDPEELYHDVYAEK
                     DYPFIKE"
     gene            complement(3508..3873)
                     /locus_tag="VFU15_12410"
     CDS             complement(3508..3873)
                     /locus_tag="VFU15_12410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718635.1"
                     /translation="MKQLHAEQLWSYSPVVIEKDYAGNGDRVYESEEMNLPGCPGDPS
                     AFRSTYFTVFKKYRNGKPESEGSFYFNAMGDHDMDSTGTWKFYEGGKPVRTKQFHSWK
                     SAYEADQHCLPDFEIPFAK"
     gene            complement(3870..4172)
                     /locus_tag="VFU15_12415"
     CDS             complement(3870..4172)
                     /locus_tag="VFU15_12415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718636.1"
                     /translation="MKNGSAHFLFAAILFSCSCSTKGKKITGNAAATDSLIVEKQEYY
                     AGGKIRSDEGHCHVRFDSLQFDRFMRQYDSSGVVSFIHFSGNRNWMATVDLFPTGR"
     gene            complement(4169..5356)
                     /locus_tag="VFU15_12420"
     CDS             complement(4169..5356)
                     /locus_tag="VFU15_12420"
                     /inference="COORDINATES: protein motif:HMM:NF019284.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718637.1"
                     /translation="MKLTIRHIIFCSVTAVIVFSSCHSRRGADVVPPQMENGIYLLED
                     DSSAATRTLFAGDTGCGAFYADTLCRIAENNFTRVSVYSYADGSLELSPELTDCGTVR
                     RDFVRYQNRGKSFAFVFHNEVVATGTFPSDTSTTYFQMKMKLLTTKDRGGQLAQLFNG
                     EKKTPDTKRVVDSTGGWTEKEYYPDGKLFSEIFFGPEVSGHDRALLRTGVARKCRTYY
                     ENGNLRYEKEQDTLTVDGIHGTWYEKHFYPQGKLMAVDLWDNNILLDREYFLNGNVKR
                     DSRDYETIESEPSLNRSYTEAGELVYEDSVMYLHVCGEGREFNHPQVIHTKYFRNGKL
                     TIEGDIFSDCGAECNWDSAGTWTFCRDGIPVDVRKFPSWQKQYADYCEAETRSAAEMK
                     NGK"
     gene            complement(5387..5875)
                     /gene="cdd"
                     /locus_tag="VFU15_12425"
     CDS             complement(5387..5875)
                     /gene="cdd"
                     /locus_tag="VFU15_12425"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014782457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEU4718638.1"
                     /translation="MKKIILSATIKEFSGDSELPGNDLDLLKRARFAAENAYAPYSQF
                     NVGAALKLRSGEVVTGNNQENVAYPSGLCAERVAVFSASANHPGEEIEAIAVSCFSVK
                     TGSAQPFSPCGACRQVLAEYEQKQGKNIRIILGGRTGNILVIDSVRDLLPLAFEAVAL
                     KK"
     gene            6106..>7074
                     /locus_tag="VFU15_12430"
     CDS             6106..>7074
                     /locus_tag="VFU15_12430"
                     /inference="COORDINATES: protein motif:HMM:NF020337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HEU4718639.1"
                     /translation="MKKITLLFALLTCGAFSARAHQDGDSIWNVSTIHDIYLDFSQAG
                     YWDSLTNNYTTDTYMRCDMTFDGRLLPSCGVKFKGNSSYNNPSDKKSFKLDLNNYVSG
                     QDYDGIKKFNLNNGFKDPTFLREKICLDFMRAHGVAAPRCTYARLYINNVYRGFYTLV
                     EEVNSKFLKQHYPDNNGNLFKGDPSGDMRWYGSADSLYYTHYELHSNETANDWSDLVH
                     LVNVVNNTSPSVYFDSLSNVLNAWSFLNYMAACNVFVNLDSYIGSGHNYFTYDDSTDL
                     RFQWIAWDVNEAFGNFNMGMQIPQLQALPYNYLSQPINRPLANKMLA"
BASE COUNT         1483 a         2087 c         1682 g         1822 t
ORIGIN      
        1 tggaaccgct tcagcgatta ttacgacagc gggttctttc accgttatca ttcgcagcac
       61 ctgggcgaca tccagtacaa ggttttctgg tacgcgttcg agcttgcctg gcagtggcag
      121 ctgacggaaa agctggattt caatttcggc ttcacgcccg ggatcccgct cgccatgtcc
      181 ttttccgccg ggctcaccta ctggccgcac cgcttcgaaa aaaagatcga gcagccgaaa
      241 gtgcacttgg gccgcagaaa aaatcccccg gtttccgcaa attgaccgcg gaaaaatttc
      301 cgttcgcctt tttccgggaa tttctgcaat ctttccggca acgtctgcgt ttgaattgac
      361 atctccccat taaacgacag accatgaaaa cgtacatcgc aaaatccatg ctcagcattt
      421 tcgctgttct tctcttcacg tccgtttatg ccggggaccc ggttgcctgc cccgggcaat
      481 cgcattcttc acgaacgcac aacggaacgg tcgctcatca tccccaacag cgcaagcgca
      541 gtgattatca tccgcagcga aaactgcaga caaaagggaa aagctatcgc cgccacagga
      601 gatgatcctc tgcccggcag cggcgtttcc cgcaaaggcg gaacgcttca ttcagttcac
      661 aaaaacttgt tgggctttgt gcaggatttc gtgagctttt gtggtgattt ttttcaggag
      721 cgcaccactt tctcatctct gcttcacggc tcccgctttt caatcggggc tgatcgtgca
      781 ccggcatcac tttttttctt ctcctgctct tgccggcttt acgcgcgatg ttttgcttac
      841 atcaaaatcg tcaccgggtt ttccagcatc atcttcagcg actgcaggaa cgccgaaccc
      901 gtcgcgccgt ccaccacgcg gtgatcgcac gacaaagtca ctttcatgat ttttccggga
      961 accacctgcc cgttcttcac cacgggaaca tccttgatcc cgccgacagc gagaatgcag
     1021 gaatccggcg ggttgatgat cgccgtgaat tcttcaatgc cgaacatgcc gaggttggag
     1081 atcgtgaatg tatttccttc ccagtcggaa ggctgcagct ttttctcttt cgctttcttg
     1141 gcgaattctt tcacctccgc gccgatctgc gcaaacgatt tctggtctgc aaaacgcacg
     1201 acaggaacca gcaggccttc ttccaccgca acggcaacgc cgatatgcac atgatggttg
     1261 tagcggatct tgtcgcccag ccatgatgaa ttcactttcg ggttgtagcg cagcgcggtc
     1321 gcgcacgcac ggatcaccat gtcgttgaac gaaattttca ccggggaaat agcgttgatc
     1381 gcttcgcgcg atttcaccgc ctgctccatg tcgatctcca tcgtcaggta aaaatgcggc
     1441 gccgaaaatt tgctttccgc cagcctgcgc gcgatcgtct tgcgcatctg cgaaaccggt
     1501 tcttccgtat agctttcttt tccgttcacc acacctgcac ccattccgaa cgagatcatg
     1561 cccgtttttc ccgggtgcca tgattccacg tcccgcttca cgacgcgccc gtactcgccc
     1621 gtcccgctga caccatgcag gtcgagtcct ttctcggccg ccagcttctt cgccagcggc
     1681 gacgctttga tccttccgtc cccgttctct tccacggcaa cggtcacggg cttcgctaca
     1741 ggctttgcag cctcctgctt tttctccgct accggtgcaa ccgttttctg ttcagcgacg
     1801 ggctccgctt ttttctcttc tttcttttcc gtttccgcct ttttctccga agcggcagct
     1861 atgatcgctt tcacatcttc gtccggtttc ccgatgatgc acaggatagc atccaccggg
     1921 gcagcctgcc ctttttccac gccgatgtag agcagcgttc cgttgtagaa cgactcgaac
     1981 tccatcgtcg ccttgtcgct ttcgatctcc gcgaggatat ctccccgctt caccgtgtca
     2041 ccgactttct tgttccacgt tgcgacaacg ccttcggtca tcgtatcgct gagcttgggc
     2101 attttaacta cttcggccat gttctgaaat aatgaatgat gaaaaatgaa gatggatcag
     2161 ttcggcattc gctgctgcgg ggtctttacg atcgcagaga gattattgat gatatcatcc
     2221 gcatccttca cctgtctggt taatttcttt tgtgtcggct ttccttttcc cggaaggcgc
     2281 agccggtaac ccggttatgc gtgcttcctt cgaagcaatg ctcatttttg aagtgaattc
     2341 tttttgatca accggctaaa gacgtttccc ggatgctgtt tctttttcat aaaaaacaac
     2401 gatccgtcat ttatcattcc ttgataaaag gatagtcttt ttccgcataa acatcatggt
     2461 aaagctcttc cggatcgggg aacggcgact cttccgcgaa tttcaccgct tcgttcacga
     2521 tgtcctcgat cttcttctcc atcgcttcca cgcctttgtc gtccagccag ccgttcgctt
     2581 tcatggtttc gatcaccgtt tcgatcgcgt cgtgcttctt gtacgtttcc acttcttcct
     2641 tcgacctgta tttcgccgga tccgacatgg aatggccgcg gtaacggtat gttttcatct
     2701 cgagcagcgt gggaccgttc cctttgcgcg catgttccgc agcagaagcg atcgcttcgt
     2761 gaacggtttc gcagcgcatc ccgtcaacct ggtaagaagg catgttgtaa gacgatccca
     2821 gcttgtacag gtcgtgcacg ttcgatgtgc gctccacgga agttcccatc gcgtaattgt
     2881 tgttctcgat gatgaacacc accggcagct tccacagcat cgacatgttg aacgtttcat
     2941 gcagcgcacc ctggcgcaca gcgccgtcgc ccatgtagca aagcgtcacg cggttgttgc
     3001 cgttgtattt gtcggcgaac gcaatgcccg caccgagcgg gatctgtccg cccacgatgc
     3061 cgtgcccgcc gaagaaatta tgctctttcg aaaacaggtg catggagccg cctttccctt
     3121 tcgagcaccc ggtgatcttc gcgaggagct cggcgaggat gaacttcggg tgaagtccgc
     3181 gcccgatcgg gtgcgcgtga tcgcggtaag cggtgatcac gcggtcgtct ttcgtgatcg
     3241 cgctttccgc accggccacg agcgcttcct gcccgatgta gaggtggcag aacccgcgga
     3301 tcttctgctg gatgtacaat tcaccgcatt tctcctcgaa acggcgcatc agcagcatcg
     3361 attcatacca ctgcgtatat acttcacggc tgaacgcggc tttctgtttt tcgggagatg
     3421 actttgtctt tgtcgccata aaatgacgtg tttttatgag tcagtaaaga taacggaaat
     3481 ggctgaatcc gggagcgggg acgcacctca cttcgcaaat ggaatttcga agtccggcag
     3541 gcaatgttga tcggcctcgt aagcgctttt ccaggaatgg aattgctttg tccgcacggg
     3601 ctttccccct tcgtaaaatt tccacgtacc cgtgctgtcc atgtcgtgat cacccatcgc
     3661 gttgaaataa aaagatcctt cgctttcggg cttgccgttc ctgtattttt taaacacggt
     3721 aaaataagtg ctgcgaaacg ccgacgggtc cccgggacat ccgggaagat tcatctcttc
     3781 gctttcatac acgcgatctc cgtttcctgc ataatccttt tcaatcacga ccggcgaata
     3841 gctccacagc tgttcggcgt gcaactgctt catcttcccg tcgggaaaag gtcgaccgtc
     3901 gccatccagt tccggtttcc tgaaaaatgg ataaacgaaa caacgcccga cgaatcgtac
     3961 tgccgcatga accggtcgaa ctgcagggaa tcgaaccgca cgtggcaatg accttcgtcg
     4021 ctgcggatct ttccgccggc ataatattcc tgcttttcca cgatgaggct gtcggttgcc
     4081 gcggcgtttc ccgttatctt ttttcctttc gtgctgcagg aacagctgaa caggatcgca
     4141 gcaaaaagaa aatgagcgga cccgttcttc atttcccgtt cttcatttcc gctgcggacc
     4201 gcgtttccgc ttcgcagtaa tccgcgtatt gcttctgcca cgaagggaat ttccgcacgt
     4261 ccacaggaat accgtcgcgg cagaacgtcc atgtccccgc gctgtcccag ttacattcgg
     4321 cgccgcaatc ggaaaaaata tccccttcta tcgtcagctt cccgttccgg aaatatttcg
     4381 tgtggatgac ctgcggatga ttgaactcgc gtccttcacc gcaaacgtgc aggtacatca
     4441 cggaatcttc gtataccagc tcgcctgctt cagtatagct gcggttcagc gaaggttcgc
     4501 tttcgatcgt ttcataatcg cgtgaatccc gtttcacatt cccgttcaga aaatattccc
     4561 ggtcgagcag gatattgttg tcccatagat ccacggccat gagctttccc tgcggataaa
     4621 aatgtttttc ataccacgtg ccgtgaattc catctacggt cagcgtatcc tgttcttttt
     4681 cgtaccggag attgccattc tcataatatg tccggcactt tctggcaacg cctgtgcgaa
     4741 gaagagcccg gtcgtgaccg gaaacttccg ggccgaaaaa aatttcgctg aacagtttcc
     4801 cgtcaggata atattctttt tccgtccagc ctcctgtgct gtccacgaca cgtttcgtat
     4861 cgggtgtttt cttttcgccg ttaaacagtt gcgcgagctg cccgccccgg tccttcgtgg
     4921 tcagcagctt catcttcatc tggaaatacg tggtggatgt atcggaagga aaagttcccg
     4981 tcgccacgac ttcattgtga aaaacaaacg caaacgactt tcccctgttt tgataacgga
     5041 caaaatcccg ccggacggtc ccgcaatccg tcagttccgg tgaaagctcc agcgatccgt
     5101 ccgcgtagct gtagacggac acccgggtaa aattattttc cgcgatgcgg cacaaggtat
     5161 cggcgtaaaa tgcgccgcag cctgtatcgc cggcgaacag cgtccgcgtt gccgcagatg
     5221 aatcgtcttc gaggagatag atcccgttct ccatttgcgg cggaacgaca tccgctccgc
     5281 gccgggaatg acaggaagaa aacacgatca cggccgtgac gctgcagaaa ataatatggc
     5341 ggatcgtgag cttcatttcc tgccgggtaa aattcctttg cggaaattat tttttcagcg
     5401 cgacggcttc gaacgcgagc ggcagcaggt cccttacgct gtcgatgacg agaatgttcc
     5461 ccgtgcgccc gccgagaatg atgcggatgt tctttccctg cttctgctcg tattccgcca
     5521 gcacctgccg gcatgcgccg cagggagaga acggctgcgc agagccggtc ttcaccgaaa
     5581 agcagctcac cgcgatcgct tcgatctctt ccccgggatg atttgcggaa gcggaaaaaa
     5641 ccgccacccg ctcggcgcaa agcccggacg ggtacgccac attttcctgg ttgttccccg
     5701 tcaccacttc tcccgatctc agcttaagcg cagcgcccac gttgaactgc gaataggggg
     5761 catacgcatt ttcagccgca aacctcgccc gcttcagcag gtcgagatcg ttgccgggaa
     5821 gctcgctgtc gccggaaaat tcctttatcg tcgcggaaag aatgatcttt ttcattggga
     5881 taaaaacaaa gatagctatt cacgcttcac cgcttccgcc ctcaggaccg ttgtccttat
     5941 gtcttcctgc ttattgtata tcaattattt acagccgatt cagccagccc ggctttccgg
     6001 ctggggttaa ttttacctgc ctgttttatt gatcctgtgt taactttctt cccgtttcac
     6061 cgcaggattt cgtataatcc ggcgtccaac aacaaaacgc cttcaatgaa aaaaatcacc
     6121 cttctctttg ctttactcac ctgcggagcg ttttccgcac gggcgcacca ggacggcgac
     6181 agcatctgga acgtcagcac gatacacgac atctacctcg acttttccca ggccggttac
     6241 tgggattcgc tgacgaataa ttacaccacc gatacctaca tgcgctgcga catgacattc
     6301 gacgggcggc tgcttccttc ctgcggggtg aaattcaaag ggaattcttc ctacaacaat
     6361 cccagcgaca aaaaatcatt caagctcgac ctcaacaatt acgtttcagg acaggattac
     6421 gacggcataa aaaaattcaa cctgaacaac gggttcaaag acccgacgtt cctgcgcgaa
     6481 aagatctgcc tcgatttcat gcgcgcgcac ggcgtggcgg ccccgcggtg tacgtatgcg
     6541 cggctctaca tcaataatgt ttaccgggga ttctacacgc tggtggaaga agtgaactcc
     6601 aaattcctca agcagcatta cccggataac aacgggaatc ttttcaaagg cgatccttcc
     6661 ggcgatatgc gctggtacgg cagcgccgat tcgctttact acacgcatta cgagctgcat
     6721 tcgaatgaaa cggcaaatga ctggagcgat ctcgtccacc tcgtcaacgt ggtcaacaat
     6781 acgtctccct ccgtctattt tgattcgctc agcaacgtgc tcaacgcctg gagctttctc
     6841 aattacatgg ccgcctgcaa cgtgttcgtc aacctcgatt cctacattgg ttccggccac
     6901 aactatttta cgtacgacga ttccaccgac ctgcgctttc aatggatcgc gtgggatgta
     6961 aatgaagcct tcgggaattt caacatgggc atgcagatcc cgcagctgca ggcgcttccc
     7021 tacaattacc tcagccagcc cataaaccgg ccgctggcca ataaaatgct tgcc
//
LOCUS       DASTSF010000430         7642 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1177671,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000430 DASTSF010000000
VERSION     DASTSF010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 7642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7642
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1177671"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            282..1529
                     /gene="rmuC"
                     /locus_tag="VFU15_12435"
     CDS             282..1529
                     /gene="rmuC"
                     /locus_tag="VFU15_12435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEU4718640.1"
                     /translation="MNQSAMEKFNRSFEEKIATLVEKTELSNKTSREEFLKNLKEISD
                     QNRQVIGEMKDSLGKSLKDFADSFDKNVKAFNDLQREKFSDLDKKQGELILKTEEKLE
                     KMRETVDEKLQKTLNERIGQSFETVSKQLLAVQEGLGEMKNLAQDVGGLKKVLGNVKM
                     RGGIGEVQLEMLLEQILAPEQFVKNYRPKESSDKVVEFAIRLPGKDDAGSFVYLPIDA
                     KFNKDAYDALLEAYDSGDAEQIRRSSKSLEDAIRKNAKDIRDKYLDPPNTTDFAIMFL
                     PFEGIFAEVVRRSSLLEEMQRDYKVIITGPTTIAAILNSLQMGFRTLALQKRSSEVWN
                     ILGAVKTEFEKFGGLLQKAQKNIHTAGNTLEELIGKRTKAINRRLRDVQGLPAGESSA
                     LLPGMENEEGTEEDDDEKNDQQN"
     gene            1550..2551
                     /gene="gap"
                     /locus_tag="VFU15_12440"
     CDS             1550..2551
                     /gene="gap"
                     /locus_tag="VFU15_12440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014681009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEU4718641.1"
                     /translation="MGKTKIAINGFGRIGRMSMRVMLERKNVEIVAINDLTDSHTLAH
                     LFKYDSVHRRFPGEVSAEPDALVINGQRIPITQEKDPSKLHWGKFGTDIVLESTGKFL
                     DRESANFHLDAGAKKVMLSAPPKSGDIKTFVFGVNDHTLSKDDVIISNASCTTNCAAP
                     MIKVLDDSWGIEDGYITTVHSYTGDQRLHDAPHRDLRRARAAAVSMIPTSTGAAKAIT
                     KIFPHLEGKLGGCGIRVPVPDGSLTDITCVLRKMPSVKEINAAFRKAAEGELKSVLEY
                     TEDPIVSVDIVGNPHSTIFDAEFTSIVGNMVKVIGWYDNEIGYSNRIVDLAVKWMSL"
     gene            2636..4735
                     /locus_tag="VFU15_12445"
     CDS             2636..4735
                     /locus_tag="VFU15_12445"
                     /inference="COORDINATES: protein motif:HMM:NF015714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HEU4718642.1"
                     /translation="MQSLLPRIPLLRLVIPFCAGIVFSYSFHPAPSVPVFALAAVLVS
                     VALLLFPQVRKRTLRLVGMEGLLVSAGMFFAGAALTEIKLGQVLSADRKEHNLLAVVF
                     RITEEPVVKERSVKVVAEIRYTKDSSGWKAASGKMLAYIRRDPEAEKLRYGDVLLVNA
                     SPQETEAPKNPGEFDYRSWLLRQGISEQLFAAQDHWKLLRNDGNWFRSAALTLRKYFM
                     RRMQESGISGDEFSVASALLLGASDHLDPDLMHAYSASGTMHVLSVSGMHVALVYVVL
                     LRLLGPFERRRGGKWPLIVLQLIFLWFYATLTGLCPSVLRSVTMLSVVIAGKAFGRKT
                     HILNSLSASALILLATDPMLLFDAGFQLSYLAVAGIVTLQPALQKLWKPEVFPLRHVW
                     TLISVTLVAQVFTFPIGLYYFHQFPTWFLLSNLLVIPLATIVMYAGLLFLAVSPFVFM
                     AGKVAVIFSFLVALLNGAVKLTEYLPFNVLHSSAWTVTEILLLYVSVVAAVLFFIRKR
                     GTWMNFSLAGFCTLLFLVSCSSVRTARRREMVFFCTPKGTAIGLKEGNAGALLTDSAF
                     NASSGTADFYAAPFFNNDGIEQFPIKNFTVKETSPSAFYRLEKNVLLFEGKTVLLLDK
                     NSAQEEYSGSYYAVLVMSSFRGNVARKLENVKMEKVIIGCGISPHRREKMRGQLQGFS
                     CAIISLSEHSFMIAAED"
     gene            complement(4713..5291)
                     /locus_tag="VFU15_12450"
     CDS             complement(4713..5291)
                     /locus_tag="VFU15_12450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718643.1"
                     /translation="MSSSRLFVRKASRHKHGVNPLLFLIILAWLVIAFVVYVNNEGAT
                     SVYLPGPLLIYFLYRSGRNRTEEVTVVIEKDNKDGDLYFYYLNEDKEKKDDYPIDEFS
                     YWYVRNLSGNKTKSFDLFVKFQCEGKDPVFLKETKVNFDVTPGWEELDEKPGSSAQTF
                     LVPDIGQLASILDSAATTENDESPAVNLPQQS"
     gene            5413..6090
                     /locus_tag="VFU15_12455"
     CDS             5413..6090
                     /locus_tag="VFU15_12455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718644.1"
                     /translation="MKRMLKIILLAVPVLCLVSLAWRYAPFFLRPSPHKTYYPDSVYR
                     EELHALNDSLGHASLRRRSLLLHSAVSERIFPFWEGTPWDFNGTTERPGNGKIACGYF
                     VTTVLRDLGFVTQRKELACMASEQMIRDLVAEKNIRRYSRFTLTDFLKDVRKKGNQLY
                     VVGLDNHTGFLSCENGQCWFIHASGGYRRCVIKEKAETSSALAFSSYRVTGCLTSDSL
                     FLAGWKK"
     gene            complement(6307..6726)
                     /locus_tag="VFU15_12460"
     CDS             complement(6307..6726)
                     /locus_tag="VFU15_12460"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEU4718645.1"
                     /translation="MSGNRTFTMIKPDAVENNYIGPILAKINEAGFRIVAMKYTRLSP
                     AMAGKFYAVHSERPFYGELVDYMSSGPIVAAILEKDDAVSSFRKLIGATDPSKADAGT
                     IRALYAKSIAANAIHGSDSDENAEIEGNFFFSQLERF"
     gene            6879..>7642
                     /locus_tag="VFU15_12465"
     CDS             6879..>7642
                     /locus_tag="VFU15_12465"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEU4718646.1"
                     /translation="MKAKSISGLRKFLSKKRNIVIVTHWSPDGDAIGSSLGLSNYLLK
                     RGHRADVIVPNDYPEFLHWMKGNRTVVDALKHRKKANTLLKDAEIVFCLDFNDLRRLN
                     DLGKTIAALPVPKVMIDHHPAPEDFAEYVLHKVSASSTAELIHEFIVADGGAKLIDRD
                     IANCLYTGIMTDTGSFRFPSTSPKTMRVAAQLLEAGAEGALNHNRIYDDNTEDRLRLL
                     GFSLSEKLAVMPELHTAFFTLTEAEQERFHYRKGDT"
BASE COUNT         1771 a         1985 c         2092 g         1794 t
ORIGIN      
        1 catctccgca ttgatcgttt cgtttaccgc ttttgattat ggaaacaggt ttgctcattc
       61 tggtttcggt tttatcgctt gtcactttgc ttcttgtggt cttgaggaaa aatgcagggc
      121 ccggtcccga catgcaggag caggtgaaag agctttcctc caatctctgg aagatcgaat
      181 cgggaatcaa ggaggatttc cgcatgaacc gggaagaact taaccgctcg cttctcgatt
      241 cccgcaaaga gctttccgaa acgcttcacc gcattgccga aatgaaccag tcggccatgg
      301 aaaaattcaa ccgctccttc gaagagaaga tcgccacgct ggtcgaaaaa acggagctgt
      361 ccaataaaac aagccgcgag gaattcctga aaaacctgaa agagatcagc gatcagaacc
      421 ggcaggtgat cggggaaatg aaggattcgc tcgggaaatc actgaaggat tttgcagatt
      481 cattcgataa aaacgtaaag gcgttcaacg acctccagcg ggaaaaattc tccgacctcg
      541 ataaaaagca gggcgagctt attctcaaaa cagaagagaa gctggagaaa atgcgggaga
      601 ctgtcgatga aaaattgcag aagacgctca atgaacgcat cggccagtcg ttcgaaacgg
      661 tttccaagca attgcttgcc gtacaggaag gtttgggcga aatgaagaac ctggcgcagg
      721 atgtcggcgg actgaaaaaa gtgctgggca atgtgaagat gcgcggcgga ataggggagg
      781 tgcagctgga aatgctgctg gagcagatcc tcgcgccgga gcagttcgtg aaaaattaca
      841 ggccgaagga aagttctgac aaggtcgtgg aattcgcgat acgcctcccg gggaaggatg
      901 atgccgggtc attcgtttat cttcccatcg atgcgaagtt caacaaggac gcttacgacg
      961 cactgctcga agcttacgac agcggcgatg ccgaacagat caggcgaagc tcgaagagcc
     1021 tcgaggacgc cattcgcaaa aacgcgaaag acatccgcga caaatacctc gatcctccga
     1081 ataccaccga cttcgcgatc atgttccttc cgttcgaagg gatttttgcc gaggtcgtac
     1141 gccgctcttc cctgctcgag gaaatgcagc gtgattataa agtgatcatt acgggtccaa
     1201 ccaccattgc cgccatcctg aacagcctgc agatgggttt ccgcacgttg gccctccaga
     1261 agcggagcag cgaagtgtgg aatatcctcg gggcggtaaa aacggaattc gaaaaattcg
     1321 gcgggctgct gcagaaagcg cagaagaata ttcacacggc cggaaatacg ctggaggagc
     1381 tcatcgggaa acgcacgaaa gcgatcaacc gcaggctcag ggacgtgcag ggacttcccg
     1441 cgggcgaaag cagcgcattg cttcccggga tggaaaacga ggaaggaacg gaagaggacg
     1501 acgatgaaaa gaacgatcag cagaactgaa ccgcataaac ttttttgaaa tgggaaaaac
     1561 aaaaattgcc attaatggtt tcgggcgcat cggccgcatg agcatgcgtg taatgctcga
     1621 acggaaaaac gtggagatcg tggcgatcaa cgacctcacg gacagccaca cgctggcgca
     1681 tcttttcaaa tacgattccg tgcaccgccg tttcccgggc gaagtttccg ccgagccgga
     1741 tgcgcttgtc atcaatggcc agcggattcc gatcacgcag gaaaaagatc cgtcgaagct
     1801 gcactggggg aaattcggca ccgatatcgt gctggaatcg acagggaaat ttctcgaccg
     1861 cgaatcggcg aactttcacc tggacgcagg tgcgaagaag gtgatgctct ccgctccgcc
     1921 aaaaagcggg gatatcaaaa ctttcgtttt cggtgtcaat gatcacacgc tgtcgaaaga
     1981 cgacgtcatt atctccaacg ccagctgcac gaccaattgc gccgcgccga tgatcaaggt
     2041 gctggacgac agctggggca tcgaagacgg ttacatcacc accgtgcatt cctacaccgg
     2101 cgatcagcgc ctgcacgatg cgccgcacag ggatctccgt cgcgcacgcg ctgcagcagt
     2161 ctccatgatc ccgacttcca cgggcgctgc caaggcgatc acgaaaattt ttccgcacct
     2221 cgagggaaag cttggcggct gcggcatccg tgtccctgtt cccgacggat cgctcactga
     2281 cattacctgc gtgctcagga aaatgccttc cgtaaaagag atcaatgcgg ctttccgcaa
     2341 agcggccgaa ggcgaactga agagcgttct cgaatacacg gaagatccta tcgtttccgt
     2401 cgacatcgtc ggtaacccgc attccaccat cttcgacgcg gaattcacgt cgatcgtggg
     2461 gaacatggtg aaagtgatcg gctggtacga caacgagatc ggctattcca accgcatcgt
     2521 ggacctggcg gtgaagtgga tgtcgctcta agcggctgat cacattttct tcccgaattc
     2581 aaacgcttaa aaccgtttaa taaaccggtg cggaaataat ttcggctgct tttttatgca
     2641 gtcgctcctt ccccgtattc cgcttctccg cctggtgatc cctttctgtg cgggaattgt
     2701 tttttcctat tcgtttcatc ccgcgccgtc ggttcctgtt ttcgctcttg ctgcagtgct
     2761 ggtgtcggtt gcgttgctcc tgttcccgca ggtgcggaaa agaacgctgc gtcttgtggg
     2821 gatggaaggc ctgctggtct ctgcgggaat gttttttgca ggagccgcgc tgacggaaat
     2881 aaagctcggg caggttcttt ccgcggaccg gaaagagcac aaccttcttg ctgtcgtgtt
     2941 caggatcacg gaagagccgg tggtgaaaga aaggtctgta aaagtggtcg cggaaatccg
     3001 gtacacgaaa gacagcagcg gctggaaagc agcttccggg aaaatgctcg cctacattcg
     3061 ccgtgacccg gaagcggaaa agctgcgtta cggcgacgtg ctgctcgtga acgcttcccc
     3121 gcaggaaacc gaagcgccga agaacccggg cgagttcgat taccgctcct ggctgctgcg
     3181 gcaggggata agtgagcagc ttttcgccgc gcaggatcac tggaaactgc tccgcaacga
     3241 cggaaactgg ttccgcagcg ctgcattgac actcaggaaa tatttcatgc ggcgcatgca
     3301 ggaaagcggg atttccggcg atgaattttc cgtcgcttcc gctttgctgc tcggcgcgtc
     3361 cgaccacctt gatcctgatc ttatgcatgc atacagtgcc tcggggacga tgcacgtcct
     3421 gtcggtttcg ggcatgcacg ttgcgctggt gtacgtcgtg ctgctgcgcc tgctggggcc
     3481 gttcgaacgg cgcaggggcg ggaaatggcc gttgatcgtt cttcagctca ttttcctctg
     3541 gttctatgcc acgcttaccg gactttgtcc gtcggtgctg cgttccgtta cgatgctttc
     3601 cgttgtcatt gcgggaaaag cgttcgggcg caaaacgcac atcctgaatt cactttccgc
     3661 ttcggcgctt attctccttg cgaccgaccc catgctgtta ttcgatgccg gtttccagct
     3721 ttcctacctc gcggtggcgg gcatcgtcac gcttcagcct gcgcttcaaa aactgtggaa
     3781 gccggaggtc ttcccgttac ggcatgtatg gacgctcatc agtgtcacac tcgttgcgca
     3841 ggtcttcact tttcccatcg ggctgtatta tttccaccag ttccccacgt ggttcctgct
     3901 cagcaatctc ctcgtcatcc cgcttgcgac gatcgtcatg tacgccggac ttttatttct
     3961 cgccgtaagc cccttcgttt tcatggcagg aaaagtggcg gtgatcttca gcttcctcgt
     4021 cgcgctgctc aacggggcgg tgaagctgac ggaatatctt ccgttcaacg tgctgcattc
     4081 ttcggcgtgg acggtgacgg aaatcctgct gctctatgtg tcggtggtcg cagcagtgct
     4141 ttttttcatt cgtaaaagag gaacgtggat gaatttttcg ctggccggtt tttgcacatt
     4201 gctgttcctt gtttcctgca gctcggtgcg gactgcgcgc aggcgggaaa tggttttctt
     4261 ctgtacgccg aaaggcaccg cgatcggtct gaaagaaggg aatgcaggag cgctcctcac
     4321 ggattccgcg ttcaacgctt cttcaggcac tgctgatttt tacgccgctc cttttttcaa
     4381 taacgatgga atagagcagt tcccgatcaa aaatttcact gtgaaggaaa cttctccgtc
     4441 cgctttttac aggcttgaaa aaaatgtatt gctgtttgaa ggaaaaacag tcctgctgct
     4501 ggataaaaat tcagcgcagg aagaatattc cggtagttat tacgcggttc tcgttatgtc
     4561 ttcgttcagg ggaaatgttg cgcggaagct ggaaaatgtg aagatggaaa aggtaattat
     4621 cggttgcggg atttcgcctc accgccgtga aaagatgcgc gggcagttgc aaggtttctc
     4681 ctgcgcgatt atttcacttt cggaacattc gttcatgatt gctgcggaag attgaccgcg
     4741 ggtgattcgt cattttccgt ggtggccgcg ctgtccagga tggaagccag ctgcccgata
     4801 tcgggtacaa gaaaagtttg tgcagaagat cccggttttt catccagttc ttcccagccg
     4861 ggagtaacgt cgaaattcac ttttgtttcc ttgaggaaga ccgggtcctt cccttcacac
     4921 tggaacttca cgaacaggtc gaaggatttg gtcttgttcc cggaaagatt ccgcacgtac
     4981 cagtaactga actcatcgat ggggtaatcg tctttttttt ctttgtcttc gttgaggtaa
     5041 taaaagtaca agtcgccgtc tttgttgtct ttttcaatga cgacggttac ctcttcggtc
     5101 ctgttcctgc ccgaacggta caggaaataa atgagcaggg gtcccggaag ataaaccgat
     5161 gttgcgcctt cattgtttac gtaaacgacg aaggcgatga ccagccacgc caggataatc
     5221 aggaacagta ggggattgac tccgtgtttg tgacgtgacg ctttgcgcac gaaaagccgg
     5281 gaagagctca ttgggttcag ggttaaagag tgaatttaca attatcccgg aaatacgtgc
     5341 agcaggtaga tgcgatgccg tgaatgttca ttccgggaag agaagcgcgg caaagcgcaa
     5401 tagcttcact ctatgaagcg catgctgaag atcattctcc tggccgttcc ggttctctgc
     5461 ctggtttcgc tggcgtggcg gtacgcgcct ttttttctcc ggccgtctcc gcacaaaacg
     5521 tattaccctg attccgttta ccgggaagaa ttgcacgcgc tcaacgatag tctcggccat
     5581 gcgtcgctcc gccggcgttc gcttctcctg cattccgcag tgagcgaaag gatatttcca
     5641 ttctgggaag gcaccccgtg ggatttcaac ggaacgacgg agaggccggg caacgggaag
     5701 atcgcctgcg gttattttgt gacgacggtg ctgcgcgatc tcggtttcgt cacgcagcgg
     5761 aaagagcttg cctgcatggc gtctgagcag atgatcaggg atctcgtcgc ggaaaaaaac
     5821 atccgcaggt attcgcgttt tacattgacg gatttcctga aggacgtccg caaaaaaggt
     5881 aaccagctgt acgtcgtggg actcgataat cacacgggat tcctgtcctg tgaaaacggg
     5941 cagtgctggt tcatccacgc gagcggtggt taccggcggt gcgtgatcaa ggaaaaagcg
     6001 gaaacttctt ctgcgcttgc gttttcttcc taccgtgtca cgggttgcct gacttcggat
     6061 tcgctgttcc tggcgggatg gaaaaaataa aaaacggcgg cccggtttaa attttcgtcg
     6121 cggcacttcc cgctttttac cacggaagcg gggagaggaa aaaagagttt ggtttcgaat
     6181 gtgttgtgaa aaaccaggta cggccttgcc cgcttttcat ctttcggtca tgatccggca
     6241 atcgaaaacg gcataaaaaa tccggcccgg gaaatccggg gccggataat tttttgtgac
     6301 gaaagatcag aaacgctcga gctgcgaaaa gaagaaattt ccttcgatct ccgcattctc
     6361 gtcgctgtcg gatccgtgga tcgcgttggc agcgatcgat tttgcgtaaa gcgcgcggat
     6421 cgttcccgca tccgccttgg aaggatcggt ggcgccgatc agtttgcgga aggaagacac
     6481 tgcatcgtct ttttcaagaa tggccgccac gatgggaccg gaagacatgt aatcgaccag
     6541 ctcgccgtag aacgggcgtt cactgtgcac tgcgtaaaat ttcccggcca ttgcgggtga
     6601 aagacgcgta tatttcatag ccacgatgcg gaacccggct tcgttgatct tcgccaggat
     6661 cggcccgata taattgttct ccactgcatc gggtttgatc atcgtaaatg tgcggttgcc
     6721 tgacatagga ttatgtttgt ttgacgttgt ttaaaatcgg ctgcaaaagt aagcattttc
     6781 acacgacgaa gattatcagc gcatcttttg aactaaattt acacccttca gagagccggt
     6841 tcgccggacg tttaccacat tccaagcttc tcaccgattt gaaagcaaaa tccatttccg
     6901 gcctgcgaaa attcctttcg aaaaaaagga acattgttat cgtcacccat tggtcgcccg
     6961 acggtgatgc catcggctcc tcgctcggcc tgtccaatta tcttttgaaa cgcggacacc
     7021 gggcggatgt catcgtcccc aacgattacc cggaattcct gcactggatg aagggaaacc
     7081 gtaccgtcgt cgacgccctt aagcaccgca agaaagcgaa cacgctgctg aaggatgcgg
     7141 aaattgtttt ctgcctcgat ttcaacgacc tcaggcgcct caacgatctc gggaaaacca
     7201 tagccgcgct tcctgttccg aaagtgatga tcgatcatca cccggcgccg gaagattttg
     7261 cggaatacgt cctgcacaag gtcagcgcga gctccaccgc agagctcatc catgaattca
     7321 tcgtcgctga cggcggcgcg aagctcatcg accgggacat cgcgaattgc ctgtatacgg
     7381 gcatcatgac cgataccggc tcgttccgtt tcccgtccac ttccccgaaa accatgcgcg
     7441 ttgcggcgca gctgctcgag gccggcgcag aaggcgcgct gaaccacaac cgcatttacg
     7501 acgacaatac cgaagaccgt ctccgcctgc tcgggttttc attgagcgaa aagctggccg
     7561 tgatgccgga gctgcatacg gcatttttca cgcttaccga agcggagcag gaacgttttc
     7621 attaccggaa aggcgatacg ga
//
LOCUS       DASTSF010000431         2133 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1612046,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000431 DASTSF010000000
VERSION     DASTSF010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1612046"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(123..1118)
                     /locus_tag="VFU15_12470"
     CDS             complement(123..1118)
                     /locus_tag="VFU15_12470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718647.1"
                     /translation="MKRTAALLSFLILVIAAGRHSQAHPHRVPAADSLEASTDSILKF
                     WLSAEAVTEEKPVCRTLWLWTSASSIDSALYYHSLLWSYRPQSLPAEFYRGELENKKW
                     NKDPLAQVLRSGSFGRTYDAWNNCWAALADAVSFEGNTQLVKVDLGDSALIVVFRPEN
                     KRHAWTVYDLEGNSLSIADALKRKDQIAVVYYSSRKTNRMKGYAGSYPPTNLPGCRSF
                     ILCNEKMIKGWGHAVPGLQDKLNSELNYLLLLKAWFAEKPGNCSVAGKNGKNTMHAWA
                     AAKNSCTTAQLCMKNFRYGSVNAGEEEIRNVIDKIRKNGPKQVNPVQKFPSRGIR"
     gene            complement(1133..>2133)
                     /gene="der"
                     /locus_tag="VFU15_12475"
     CDS             complement(1133..>2133)
                     /gene="der"
                     /locus_tag="VFU15_12475"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006264649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEU4718648.1"
                     /translation="ADIVRRSKKKIFLVANKVDTGDKTYLASEFYSLGLGDPFPVSGT
                     NGSGTGELLDELVKHLGPEEENAEEEIPRIAIVGRPNAGKSSIVNVLIGEEKHIVTDI
                     AGTTRDAIDTRFKGFGHDLLLIDTAGLRKKTKVHEDIEFYSVMRSIRAIEECDVCMLV
                     VDAKEGFEGQDLNIFSLAEKNKKGVVILVNKWDLVEDKTNKTTKEMEEEIRHKLQPFR
                     DVPIVFTSAVTKQRILKALEIAIKVYENRKKRISTSQLNEVMLPLIENYPPPAVKASY
                     VKIKYVTQLPTKTPVFAFFCNHPQYIQDSYKRFLENKLRENFDFNGVPVSVVFRKK"
BASE COUNT          438 a          582 c          564 g          549 t
ORIGIN      
        1 gctggtgctg ggcgtgtatg gagatgaagg cgaagtcatc ctgcttcagc ccggacggaa
       61 agtgcagaac ggcgggaagg tcgggtgaat gaaacggcaa taatgggttt cgcgctttcc
      121 cgtcagcgaa tgccgcgcga aggaaatttc tgcaccgggt tcacctgttt cggcccgttc
      181 ttcctgatct tatcgatcac attcctgatc tcttcttccc ctgcattcac cgatccgtaa
      241 cggaaattct tcatgcacaa ctgtgcggtc gtgcaggaat ttttcgccgc cgcccatgca
      301 tgcatcgtgt tctttccatt tttcccggcg acggaacagt ttcccggttt ctccgcaaac
      361 caggctttca gcagcagcag gtagttcagc tcgctgttca gtttatcctg cagaccggga
      421 actgcatgtc cccatccttt gatcattttt tcattgcaga gaatgaaaga acggcaaccg
      481 ggcaggttag ttggcggata agatcccgcg taccctttca tccggttggt ttttcgcgag
      541 gaatagtaca ccaccgcgat ctggtctttt cttttcagcg catctgcaat ggaaagtgaa
      601 tttccttcca ggtcgtaaac cgtccacgcg tgccgcttgt tttccggccg gaaaaccacg
      661 atgagcgcgc tgtccccgag atcgaccttg acgagctgcg tgtttccttc gaaagaaacg
      721 gcatccgcca gcgcggccca gcaattgttc catgcgtcat acgtgcggcc gaaagaaccc
      781 gaacggagca cctgcgcaag cggatccttg ttccactttt tgttttccag ttcgccgcgg
      841 taaaattccg cgggcaacga ctgcggcctg tagctccaca gcagggaatg gtaatacagc
      901 gcggaatcga tggacgatgc gctcgtccac agccagagcg ttctgcaaac cggtttttct
      961 tccgtcaccg cttccgcgga aagccagaac ttcaatatcg aatcagtgga agcttcgagg
     1021 gaatccgccg caggaacgcg gtgtggatgc gcctgcgagt gccggcccgc agcgatgaca
     1081 agaatgagaa acgaaagaag agctgctgtt cgtttcatga atgaaagcgc tgttattttt
     1141 tccggaaaac aacacttacc ggtacgccgt tgaaatcgaa attctcgcgc agcttatttt
     1201 cgaggaaacg cttgtaggag tcctgtatgt actgcggatg attgcagaaa aatgcgaaca
     1261 cgggcgtttt cgtcggaagc tgcgtgacgt attttatttt cacgtagctc gcttttacag
     1321 ccgggggcgg gtaattttcg atcagcggga gcatcacttc attcagctgc gaggtggaaa
     1381 tgcgtttctt ccggttctcg taaactttaa tggcgatctc gagcgccttg aggatgcgct
     1441 gcttcgtcac tgcggacgtg aacacgatcg gcacgtcgcg gaacggctgc agcttgtggc
     1501 ggatctcctc ttccatttct ttcgtcgtct tgttcgtctt gtcctccacc aggtcccatt
     1561 tgttgacgag aataacgacg ccttttttat ttttttccgc cagcgaaaat atgttcaggt
     1621 cctgtccttc gaaaccttcc ttcgcatcaa cgacgagcat gcacacgtcg cattcttcga
     1681 tggcgcggat gctgcgcatg acggaataga attcaatgtc ttcatgcact ttcgttttct
     1741 tccgcagtcc cgccgtatcg atgagcagga gatcgtgccc gaaacctttg aaccgcgtat
     1801 cgatcgcatc gcgcgttgtt ccggcgatgt ccgtcacaat gtgcttttcc tcgccgatga
     1861 gaacgttcac aatggatgat ttcccggcgt tgggtcgccc gacgatcgca atgcgcggaa
     1921 tttcctcctc cgcattttct tcttccgggc cgaggtgttt caccagctcg tcgagcagct
     1981 ccccggttcc gcttccgttc gtcccggaaa cagggaacgg atcgccaagg ccgagcgaat
     2041 agaattcgga agcgagatag gttttgtcgc ccgtgtcgac tttattggcg acgagaaaaa
     2101 tcttcttctt gctgcggcgg acgatgtcgg cca
//
LOCUS       DASTSF010000432         3677 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_962865,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000432 DASTSF010000000
VERSION     DASTSF010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3677
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_962865"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1121
                     /locus_tag="VFU15_12480"
     CDS             <1..1121
                     /locus_tag="VFU15_12480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010609084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HEU4718649.1"
                     /translation="LRGGAAGGGTRMRKGLDKREVESLAKTMEVKFTVAGPPIGGAKS
                     GINFDPADPRRKGVLERWFRAVTPLLKNYYGTGGDLNVDEIHDVIAITREYGLLHPQE
                     GVVRGHFGADTEGISKRIANLRNGVTKKIEDSTYTPGDNYTVADMVTGYGVAESVRHY
                     YALWGGSVKGKKVLVQGWGNVASAAAWYLACMGAVITGIVDREGGIISAEGLDEKEVR
                     KLFLSKEGNRLNAQHMLSAADIQEKVWDCGANIFIPAAASRLVTKAQVDRMIANGLEV
                     ISCGANVPFADKEIFFGPTGLHADEAVSVIPDFIANCGMARVFAYLMGENVSLTDASI
                     FGDVSQTIRRALEKTHAASPAKTGIARAAFGIALRQLV"
     gene            1122..2237
                     /locus_tag="VFU15_12485"
     CDS             1122..2237
                     /locus_tag="VFU15_12485"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEU4718650.1"
                     /translation="MQKLQEFLDSTFIAPNTVRDWWFFVLIILSGLVLRTLVARVLTK
                     IIFSVFRKKPTDTVGFQKLFELVKKPWHYFILFVVIYIACRQLTWPPEWSMTPPGELG
                     LRMVLERLIRALIEISFTWILLRLVDFFGLVLHHRAIQTTGKDDDQLVPFLRESIKVI
                     VVILAVFFLLGSVFHLNVASLVAGLGIGGIAVALAAKESLENLIASFTIFLDKPFMVG
                     DIIQMGDKTGTIEKIGFRSTRIRGEDRNYVTIPNHKLVENEIVNLTMRTARRVRFTVG
                     LVYSTRPEQIRKIVADILHFIETHPQTSFEGDNRVNLFELSSSSIDVQVTCYIPDLDY
                     TLFLKTKEEINFAIMDIVEKNGSSFAFPSTSVYIEKK"
     gene            2541..3344
                     /locus_tag="VFU15_12490"
     CDS             2541..3344
                     /locus_tag="VFU15_12490"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEU4718651.1"
                     /translation="MDEKYVLSASFNELVFENRNKKYGAYYIRRNYRRSVMAACVIAS
                     SAFCSAMGLYFLTQDNADAMTPPTEYVTHVVDLTQTTDVVNPPKEKQEVQKRLDPPAP
                     KAPKATADLTTPVVTTTQPVTPPPANNEAGGKPTGVEGGTGTGDPVKNPCLDCEQDSA
                     PAKPEPPLVRWASNPPSFPGIDKFFADNTHYPQAAKDEDIEGTVWLTWVVTTTGQVKD
                     VQVLKGANPILDREALRVAKLMPAWTPGDDNGTPVNFIYNKPIRFVLSK"
BASE COUNT          898 a         1004 c         1001 g          774 t
ORIGIN      
        1 cactgcgcgg gggtgctgcg ggaggcggaa cacgcatgcg gaaaggtctc gacaaacgtg
       61 aagtggaatc actggcgaaa acgatggaag tgaaattcac cgtggcgggt ccgccgatcg
      121 gcggagcaaa atccgggatc aatttcgatc cggccgaccc gaggagaaaa ggcgttctcg
      181 aacgctggtt ccgcgccgtc actcccctgc ttaagaatta ttacggaaca ggcggcgatc
      241 tcaacgtcga cgaaatacat gacgtcattg ccattacgcg cgaatacggt ctgctgcatc
      301 cgcaggaagg cgtggtgcgc gggcatttcg gcgccgacac ggaaggaatt tccaaacgca
      361 tcgctaatct tcggaacggc gtaacgaaaa aaatagaaga cagcacgtac acgccgggcg
      421 acaattacac cgtcgccgac atggtcacag gttatggcgt ggcggaatcc gtccggcatt
      481 attacgcttt gtggggcgga agcgtgaaag gaaaaaaagt tctcgtgcag ggatggggca
      541 acgtcgcttc cgccgccgcg tggtatctcg cctgcatggg tgccgtgatc acgggtatcg
      601 tggaccgcga gggcgggatc atcagcgcgg aaggacttga tgaaaaagaa gtgcggaaac
      661 ttttcctttc gaaagaaggc aaccggctga atgcacagca catgctctcg gcggcggata
      721 tccaggaaaa agtatgggac tgcggtgcga acattttcat cccggctgcc gcctcgcgtc
      781 tcgtgacaaa agcgcaggtc gaccggatga tcgcaaacgg gctggaagtg atctcgtgcg
      841 gagcgaacgt tccgttcgcc gataaagaaa tctttttcgg tcccacgggt cttcacgccg
      901 acgaggccgt gagcgttatt ccagatttta tcgcgaactg cggaatggcg cgcgtgtttg
      961 cttatctcat gggtgaaaat gtttcactca cggacgcttc cattttcggt gatgtttcgc
     1021 agacgatccg ccgggcgctg gaaaaaacac acgcggccag tcctgcgaaa accggcattg
     1081 cacgcgccgc tttcgggatc gcgctgcgtc aacttgtata aatgcaaaag ctgcaggaat
     1141 ttctcgacag cacattcatt gcaccgaata cggtacgcga ctggtggttc ttcgtcctga
     1201 tcattctttc cgggctggtc ctgcgcacgc tggtggcgag agttctcacg aagatcattt
     1261 tttccgtttt caggaagaag ccgacggaca cggtcggctt ccagaagctg ttcgagctcg
     1321 tcaaaaagcc ctggcattat ttcattcttt tcgtggtgat ctacatcgcg tgccggcagc
     1381 ttacctggcc accagaatgg agcatgacgc ctccggggga actgggtttg cgcatggtgc
     1441 tcgaacggct aatacgcgcg ctgatcgaga tctcgttcac ctggatcctt ctccggctgg
     1501 tcgatttttt cgggctggtg cttcatcatc gcgccatcca gacgacaggc aaagacgacg
     1561 accagctggt cccgttcctg cgcgaatcga tcaaggtcat cgtcgtcatt cttgcggtct
     1621 tcttcctgct cggcagtgtt tttcacctga acgtcgcatc actggtggcg ggactcggca
     1681 tcgggggaat tgcggtggcg cttgccgcga aggaaagtct cgagaacctc attgccagct
     1741 ttacgatatt tctcgacaaa cctttcatgg tcggcgacat catccagatg ggcgataaga
     1801 cggggacgat cgaaaagatc ggcttccgca gcacgcgcat acgcggggaa gaccggaatt
     1861 acgtgacgat accgaaccac aagctggtgg agaacgagat cgtgaacctc accatgcgca
     1921 cagcgcgacg tgtgcgtttc actgtcgggc ttgtttattc cacgcgcccg gagcagatca
     1981 ggaagatcgt tgccgatatc cttcatttca tcgaaacgca tccgcagacc tcattcgaag
     2041 gcgacaaccg cgtaaacctg ttcgagctca gcagcagctc gatcgacgtg caggtaacct
     2101 gctacattcc cgatctcgat tacacgcttt ttctgaaaac aaaagaagag atcaatttcg
     2161 cgatcatgga catcgtggaa aagaacggat cttcttttgc gtttccttca acatcggtgt
     2221 acatcgagaa gaaataacgc atttcccatt ccacttgctt tttcccgtcc gccacttgca
     2281 aagcaccgtt tccgcatgtg caacatcctt cctactcttg cataaacaaa gcgaacgcgc
     2341 tttttctttt ccgaatgatc tgactctact cctgcagtaa caaaacgcac gcaacgcatc
     2401 ctcctcaccg gtacccgaac cggtgatcac atcctccgcc gcgtgcagca cattcccgac
     2461 gtgtaaatgc aggtgcattt ttttgcgcag aaaaatgcag cgaaacagat tatgtctaac
     2521 ccaattaaaa ctttgatcgt atggacgaaa aatatgtcct ctccgccagc ttcaatgagc
     2581 tggtgttcga gaaccgcaat aaaaaatacg gcgcgtacta catacgccgt aactaccgga
     2641 gaagcgtgat ggccgcgtgc gtgatcgcat catccgcttt ctgctccgcg atggggctct
     2701 atttcctgac gcaggataac gccgatgcca tgacgcctcc gacagagtac gtcacgcatg
     2761 tcgtggatct tacgcaaacg actgatgtcg taaatccgcc gaaggaaaag caggaagtgc
     2821 agaaacgact cgatccgccg gcgccgaaag cgccgaaggc gacagcagat ctgaccacac
     2881 ctgttgtaac gaccacccag ccggtcacac ctccgcccgc gaataatgaa gcaggcggaa
     2941 aaccgaccgg tgtggaagga ggaacaggaa ccggggaccc ggtaaaaaat ccctgcctcg
     3001 attgcgaaca ggattcggcg cctgccaaac cggaaccgcc gcttgtccgc tgggccagca
     3061 acccgccttc gttccccggg atcgacaagt tcttcgcgga caacacgcat tacccgcagg
     3121 ctgcgaaaga tgaagacatc gaagggaccg tgtggctgac ctgggtggtg acgacgaccg
     3181 gacaagtgaa agatgtgcag gtgctgaaag gtgcaaatcc cattctcgac cgtgaagcgc
     3241 tgcgcgtggc gaagctgatg cccgcctgga cgccggggga cgataacggc acaccggtaa
     3301 acttcatcta caacaaaccg atccgtttcg tcctctctaa gtaaattcta acggagcgaa
     3361 aacgttgcga gaaatcgcgc gaagatcttt gctgccggca gtgaagatcg gcgaaggctt
     3421 gccgggaatg aacacgcgag tgttgaaaat aggctcaggg actttcccgt gagcctttcg
     3481 ctttgaaata atttcgatga aaacacgtta ttaattcaag aaaaccagga gcgaaaaaat
     3541 gtctcacttc cttttacttc aggccgcaaa tattgcctcg gactcgttaa ataaagcaaa
     3601 cgccgccgtc aacaccgcgc agccggcaca ggaaagcatc acgatgatgg atatgctgcg
     3661 cgacagctgg ttcgtga
//
LOCUS       DASTSF010000433        12929 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1593907,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000433 DASTSF010000000
VERSION     DASTSF010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 12929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12929
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1593907"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(182..940)
                     /gene="rlmB"
                     /locus_tag="VFU15_12495"
     CDS             complement(182..940)
                     /gene="rlmB"
                     /locus_tag="VFU15_12495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014680199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEU4718652.1"
                     /translation="MKKETHLSDDTLVYGIHAVSEAIRAGKEFDKLFIQDGLKGDLFS
                     ALKPLIREAGIFCQFVPVQKLNKITGGKNHQGVAGFLSQIGYHSIGQVVPSLFESGET
                     PLVLILDRITDVRNFGAIARTAECAGAGAIVIPSRGAAQVNADAVKTSAGALHKIPVC
                     REDNLKETIEYLKQSGFRIAACTEKASQPLYEADLTGPLAVIMGSEEDGISPEYLKRS
                     DVRIQIPMRGTISSLNVSVAAGIVLFEVLRQRRA"
     gene            complement(942..2249)
                     /locus_tag="VFU15_12500"
     CDS             complement(942..2249)
                     /locus_tag="VFU15_12500"
                     /inference="COORDINATES: protein motif:HMM:NF040439.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HEU4718653.1"
                     /translation="MISGQKKRSGILFTLGAAFFVLLFSAALFDSCYTSQPSVSRQNF
                     SKYYKPEEKVLRPGYVLFNVTDSMSRLYFTVNSTDLLYSKNGGSDDYTARVLLSYVMH
                     PVDLPKMVADSGHSVMTDVSPPGENKQLASWVDIDVPAKGKYFLEITFRDMNKLTISY
                     ELLYLDQSGPDSPNDFLLVSKETDSPLFRNYVDPGESFMIRCKNSYQGKLFVRRYGNS
                     PYPAHPPYSTYKYQDDAVPDSSWWMSDSAHTSLSLSRKGWYLFSTGENSTGGIVVARF
                     SPGFPVISDPRDLLYPLRYHTTKEEYTKMDTASDTKKEVDQFWLDKSGSEERAREVIR
                     NFYNRVEAADKLFSTEKEGWKSDRGMIYIIYGPPQNVYRNVSSETWIYGNDFGPGSVS
                     FVFDHVANAPTDQEFELARSQDYRVSWTQEVDEWRQGHVTTMH"
     gene            complement(2404..4140)
                     /locus_tag="VFU15_12505"
     CDS             complement(2404..4140)
                     /locus_tag="VFU15_12505"
                     /inference="COORDINATES: protein motif:HMM:NF018899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HEU4718654.1"
                     /translation="MRLPVPVIVFVLLRGMLSAQTTFSLDVIEPGNEHALKKIDYAKT
                     FRSKAERTKTMNDILVTLWNESYIAARYDSVREDSSSATAYLDPGVKYKWARIGKGNV
                     DEQILSATGFREKTFRNRAIRFKDVAKVEENILEWCENHGYPFASVQLDSVRFDNENT
                     ISAQLSLNKNRYTKIDSIDVDGSLKISRPFLYNYIGIRPGDAYDESKLVALSKRLREL
                     AFAKVTKSYTILFTEKYTKLTLYLDKKRAGQFDGVVGFLPDSRTGKIQFSGDARLKLQ
                     NSFGHGEVLELTWSRLRVQTQDLKVHVNYPYLLHTPVGIDYTIKLYRQDTTFIDVNQA
                     IGLQYMFSANTSLTAFVKRRSSTLLSTSLYEASTVLPDFADISTNAYGLGFHREDLDY
                     RFNPRRGYAFTITADIGNRNIKENPRLNPVVYQNLTLHSVQYGSELDAEKFFPVGKRS
                     TIMTGLKSAWLYNSQSIFRNELYRIGGLKTLRGFDEESIYASSYAIGTVEYRFLLEEN
                     SWWFVFTDACWYENNSRNTFVTDTPFGFGTGITFETKAGIFALTYALGSQFGNPVQFK
                     DGKIHVGFVGMF"
     gene            complement(4199..4834)
                     /locus_tag="VFU15_12510"
     CDS             complement(4199..4834)
                     /locus_tag="VFU15_12510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEU4718655.1"
                     /translation="MKKIILLTVFSASVLSCAAQFFQGFGIMAGATAGRQRWDFYSPD
                     SKSKSKYLLRYNGEIFAEFFDNPTFRWVTELQYNVKGAKFEGQTTNPVGTGTVTNKYT
                     NQYAAWNNYLMIRREMVSIIPYAKVGPRLEYVFSSQQSFKKFHVTGALGAGVEFVAYG
                     PVKFLTEAWWVPDLAHSYTSDHFDIRQHCWELRVGLKFTFSHGESCPRVYT"
     gene            complement(5130..6008)
                     /gene="fabD"
                     /locus_tag="VFU15_12515"
     CDS             complement(5130..6008)
                     /gene="fabD"
                     /locus_tag="VFU15_12515"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006257278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEU4718656.1"
                     /translation="MKKAYIFPGQGSQFPGMAKELYETNSVAKEMLERANEILGFRIT
                     DTMFSGTDEELKQTKVTQPAIFLHSVSLAATLGDFHPDMVAGHSLGEFSALVANKTLA
                     FEDALVLVSKRAMAMQKACEMQPSTMAAVLGMDDGKVEEICASVTDDVVVAANYNCPG
                     QLVISGSVTGINKAIELLKAAGAKRALVLPVGGAFHSPLMEPARVELEAAIRATKFSA
                     PVCPVYQNVNAKPVLDPAEIQQNLIAQLTAPVRWTQTVQNMVADGATNFIEVGPGKVL
                     QGLVKKISAGAEAVSA"
     gene            complement(6182..6901)
                     /locus_tag="VFU15_12520"
     CDS             complement(6182..6901)
                     /locus_tag="VFU15_12520"
                     /inference="COORDINATES: protein motif:HMM:NF013214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEU4718657.1"
                     /translation="MYNNFQNEETIGKEYITEQATRAFVAQVFGWMFGALLITAGIAF
                     AFANIPSLTSILFDAATAKPTAAFWIATFSPIAVVMAVSIGLDRMSTALVASLYILYS
                     ALLGVSLCVIFLAFDPLLIVKAFALTAGTFGIMAILGYTTKTDLTRFGSILMIGVFGI
                     VVSMIINWFMHSSGFSYLIDIACLVVFTGLVAFKMQQIKTIGQQVGTSRPKLAIWGAL
                     TLYITFINLFLTILRLLGSRR"
     gene            complement(6921..7529)
                     /gene="folE"
                     /locus_tag="VFU15_12525"
     CDS             complement(6921..7529)
                     /gene="folE"
                     /locus_tag="VFU15_12525"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015809948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEU4718658.1"
                     /translation="MEEEFGYTKIDKYNKSAIESLSRQYKLILEKIGEDPEREGLAKT
                     PERVAKAMQFLTHGYDLNPAEILRSAMFREEYSQMVIVKDIEVYSMCEHHMLPFFGKA
                     HVAYIPNGCVVGLSKIPRVVDAFARRLQVQERLTNEIRDCIQDTMKPLGVGVVIEAQH
                     LCMQMRGIQKQNSVTTTSAFTGEFLQETTRSEFIKLIGSKLH"
     gene            complement(7577..7996)
                     /locus_tag="VFU15_12530"
     CDS             complement(7577..7996)
                     /locus_tag="VFU15_12530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002998549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HEU4718659.1"
                     /translation="MIYITRRERFTAAHKLFRPEWSDEKNAEIFGGCANPNWHGHNYE
                     LFVTVKGNIDPSLGYVMNLKDLSGMIRKLVTEKLDHRNLNLDVDFLEGVMTSTENIAV
                     AIWNQLEKPVAESGAKLHGVKLVETENNYVEYFGSGT"
     gene            8140..8820
                     /gene="mqnB"
                     /locus_tag="VFU15_12535"
     CDS             8140..8820
                     /gene="mqnB"
                     /locus_tag="VFU15_12535"
                     /EC_number="3.2.2.26"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="futalosine hydrolase"
                     /protein_id="HEU4718660.1"
                     /translation="MKILVVSATYMEIAPLVAKMKFTGEAGPRLRAYEAGRHHVDVLI
                     SGVGMTATAYWLGHVFAKEKYEAAFNFGTCGSFDPSFAPGTVVQVISDRISEAGAEDG
                     DKFLSVSELNLLGENEFPYKWGQLVNLHPMQNPSLSSLQQVNGITVNTVHGAEHSIHK
                     VKERYKPQTESMEGAAFAYACLVENIPYAQVRAVSNIVERRNRDAWKMAEAIRNLNEK
                     AAEILDNV"
     gene            8886..9725
                     /locus_tag="VFU15_12540"
     CDS             8886..9725
                     /locus_tag="VFU15_12540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014682370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-6-naphthoate synthase"
                     /protein_id="HEU4718661.1"
                     /translation="MKIALGFSPCPNDCFIFDAIVNKRIDTQGIDFDIVMEDVETLNR
                     KAFSSSIDVTKLSYHAFAWCAEKYVLLDSGSALGRNCGPLLISKREISIDEVKNGKLN
                     IAIPGKYTTANFLLGYAFPEAKNKTELVFSEIEKALLEEKADAGLIIHENRFTYEAKG
                     LRKIIDLGEFWEETTKAPIPLGGIAMRREFPADVQKKVSALIRESVEYAFAHREESLP
                     FIRAHAQEMSEEVMYKHIDLYVNGFSVTLGTEGRRAVEILFGKALEKKIIPPFSGDLF
                     VAR"
     gene            9767..10528
                     /locus_tag="VFU15_12545"
     CDS             9767..10528
                     /locus_tag="VFU15_12545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008511671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEU4718662.1"
                     /translation="MHFLHDILSDPAKALLIIFNLVIIESLLSVDNAAVLATMVMDLP
                     KEQRGKALRYGIFGAYIFRGASLVLAAWLVHIWWLKAAGGAYLLYLSVNYFAAKATPE
                     KEDDPLNKSESWIYKKTKGFLSAFWATVILVEVMDMAFSIDNVFAAVAFTDNILLICA
                     GVFIGILAMRFVAQGFVKLMEKYAFLETSAFIVIFLLGLKLSVTLWTHFTPLSPLALF
                     LESHTADLYVSIGTMTIFLLPLLTSALFGWPKREK"
     gene            10558..11523
                     /gene="rfaD"
                     /locus_tag="VFU15_12550"
     CDS             10558..11523
                     /gene="rfaD"
                     /locus_tag="VFU15_12550"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HEU4718663.1"
                     /translation="MKVITGAAGFIGSCLVSKLNEEGAEDIILVDDFSREDKNRNLEG
                     KIFLKKIHRDDFPKWMQEHGGKLDAVFHIGARTDTTEFDKSVFDRLNLNYSKAIWELC
                     CRFDIPLVYASSAATYGLGEFGYKDDHAVVEKLKPLNPYGESKNDFDRWVLKQKHHPP
                     HWYGLKFFNVYGPNEYHKGRMASVIFHAFGQISETGAMRLFRSHNAKYKDGGQLRDFV
                     YVKDVTEVIDFLADQYPESGIYNLGTGKARTFLDLARSTFSAMGREERTTFIDTPEDI
                     RDKYQYFTEADMSKLKKAGYKNEFASLEDGVRDYVQNYLAGHNYL"
     gene            11544..12206
                     /locus_tag="VFU15_12555"
     CDS             11544..12206
                     /locus_tag="VFU15_12555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718664.1"
                     /translation="MRSIFYTVTLAALLSSCGNAEKDPRRDSMFRDSVAAAKKDPAPS
                     AKENNAGAIPVADVMPSTCDSSIYEDYLPSSLQDFLEKDFSGWKLVSPSLWSGYWFDK
                     YKTDSSLVDLFPADFNCDDKTDYALLMYNAERKETGLWIFIAGKAGFSAEKADVWENQ
                     PEADLGLYAIQRAPDRAVTDVYENKTVKPDCDAIDFVKFESASRVYYRANGKYLYVQT
                     SD"
     gene            complement(12258..>12929)
                     /gene="yidC"
                     /locus_tag="VFU15_12560"
     CDS             complement(12258..>12929)
                     /gene="yidC"
                     /locus_tag="VFU15_12560"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEU4718665.1"
                     /translation="KMRVLKPEIDEINEKYKGGDQLKKQQATMELYKKAGANPASGCV
                     PLLLQIPILFSLIRLFPASFEIRQQGLWWAKDLSTYDSAWNFSHSIWLLGDHVSLFAL
                     LMTASTILYTWMNQQMLTPGSTQMPGMKWLVYLMPVVFFFALNSYSAALSYYYFVSNI
                     ITFGQMFLMRRFVDEKAIHAKIQENKKKQVTKKPGLMQRLEQAQRKRMEELQQQQKGQ
                     KGRKK"
BASE COUNT         2912 a         3379 c         3507 g         3131 t
ORIGIN      
        1 ctgcggcctg gatttcaaaa agaaaaaagc gaggcgcgat ctgaagcccg gaccatagct
       61 ggcagtggcg gcttcgtttt ccggtgaaat gtaccgtgct gtcaaaaagc cggcgttctt
      121 cggggactga tctttcttca ggccggacat gcagcaaacg cgaactgtaa gccgcgagca
      181 gctacgctct ccgttgccgc agcacttcga acaaaacaat tccggccgcc accgaaacgt
      241 tcagggaaga aatggttccg cgcatgggaa tttgtatgcg cacgtcggaa cgtttcaaat
      301 attcaggtga gatgccgtct tcttccgagc ccatgatcac ggcgagcgga ccggtgagat
      361 cggcttcgta cagcggctgg ctggcttttt ccgtgcaggc tgcaatgcgg aatcccgact
      421 gcttcaggta ttcgatcgtt tctttgaggt tgtcttcgcg gcacacggga attttgtgca
      481 gcgcgcctgc ggatgttttc accgcgtcgg cgttcacctg cgcggcgccg cgtgaaggaa
      541 tgacgatcgc gcctgcgcct gcgcattcgg ccgtgcgtgc gatcgcgccg aagttgcgca
      601 catcggtgat gcgatcgagg atgagcacga gcggcgtttc accgctttcg aaaagcgaag
      661 gcaccacctg tccgatggaa tgataaccga tctgcgaaag gaatcccgcg acaccctggt
      721 gattttttcc gcccgtgatc ttgttgagct tctgcacggg aacgaactgg cagaaaattc
      781 ccgcttcgcg gatcagcggc ttcagcgcgc tgaacaaatc tcctttcagt ccgtcctgga
      841 tgaacagctt gtcgaattcc ttcccggcgc gtatggcttc gctcacggcg tggatgccgt
      901 aaacgagcgt gtcgtcggac aaatgcgttt cttttttcac gttaatgcat ggtggttaca
      961 tgtccctgtc tccattcgtc cacttcctgc gtccagctca cgcggtaatc ctggctgcgt
     1021 gcgagctcga actcctggtc ggtgggtgcg ttggcgacat gatcgaaaac aaagctgacg
     1081 ctgccgggac cgaaatcatt cccgtagatc caggtttcgc tggaaacatt gcggtacacg
     1141 ttctgcggcg gaccgtaaat aatgtagatc attccgcggt cgcttttcca gccttctttt
     1201 tccgtggaaa ataatttgtc ggcggcttcc acgcggttgt agaaattgcg gatcacctcg
     1261 cgcgcgcgtt cttcgctccc ggatttatcg agccagaact ggtctacttc ttttttcgtg
     1321 tccgaagcgg tatccatttt cgtgtattct tctttcgtgg tgtggtagcg cagcgggtaa
     1381 agcaggtcgc ggggatcgct gatcacggga aagccgggac tgaaccgcgc aacgacgatt
     1441 ccgcctgttg aattttcgcc ggtggaaaag aggtaccatc ctttgcgtga aagcgacagg
     1501 cttgtatgtg cagaatcgct catccaccac gaagaatcgg gaactgcgtc atcctggtat
     1561 ttatacgtgg agtagggcgg atgcgcagga taaggtgaat tcccgtagcg gcgcacgaac
     1621 agttttcctt ggtacgaatt tttgcagcgg atcatgaacg actcgcccgg atccacataa
     1681 ttccggaaga gcggtgagtc cgtttctttc gaaacgagaa gaaaatcatt cggggaatca
     1741 ggtccgctct ggtcgaggta aagcaattcg tagctgatcg tgagcttgtt catatcgcgg
     1801 aaggtgatct cgagaaaata ttttcctttc gcaggaacat cgatgtccac ccaggaagcg
     1861 agctgcttgt tttcaccggg cggacttaca tccgtcatca cggagtgacc gctgtcggcg
     1921 accatcttcg gaagatcgac aggatgcatt acgtaggaaa gcagcacgcg tgccgtatag
     1981 tcgtcgctgc cgccgttctt cgaatagagc aggtcggtgg aattgacggt gaagtaaagc
     2041 ctgctcatgc tgtccgttac gttgaagagc acataaccgg gtcgcagcac cttttcttca
     2101 ggcttgtaat attttgaaaa gttctggcgt gaaacggaag gttgtgacgt gtagcaggaa
     2161 tcgaacaacg cagcggaaaa aagtaaaacg aaaaatgccg cacccaaagt gaacaggatg
     2221 ccgctacgtt ttttttgtcc gctgatcatg ggtgaatgtt ctccgaaagc aaatttaacc
     2281 aaaagagcgg ggaaggggat ttggaaggag gttatctgtg gaaaatgatg aatgggaacg
     2341 actgtataac gcacatacct ggatgaaaaa cgaaaacggg aaatgagaaa ctggaaacag
     2401 tatttaaaac atccccacaa atcccacgtg tatcttcccg tccttgaact gaacgggatt
     2461 tccgaactgg cttcccagcg catacgtgag tgcgaaaatt cccgccttgg tctcgaacgt
     2521 gatgccggtg ccgaatccga acggcgtgtc ggtgacgaac gtgttgcggc tgttgttctc
     2581 gtaccagcat gcgtcggtga aaacgaacca ccatgaattt tcttcgagca ggaaacggta
     2641 ttcgaccgtg ccgatggcat aagacgatgc gtaaatggat tcctcgtcga acccgcgcag
     2701 cgttttgagt ccgccgatgc ggtacagctc attgcgaaag atcgactggc tgttgtagag
     2761 ccacgccgac ttcagcccgg tcatgatcgt gctgcgtttt cctaccggga aaaatttttc
     2821 cgcatccagc tcgctgccat actgcacgct gtgcagcgtc aggttctggt aaacaaccgg
     2881 gttcagccgc gggttttctt tgatgttgcg gttgccgatg tccgctgtga tcgtgaatgc
     2941 atatcctctt ctcgggttga aacggtaatc gaggtcttcc cggtgaaaac cgagcccgta
     3001 tgcgttcgtg ctgatgtccg cgaaatccgg aagaaccgtc gacgcttcgt aaagcgaagt
     3061 ggaaagcagc gtggagcttc tccgtttgac gaacgcggta aggctcgtgt ttgcgctgaa
     3121 catgtactgc agcccgatcg cctggttcac gtcgatgaac gtggtgtcct gcctgtacag
     3181 cttgatcgtg tagtcgatgc ctacgggcgt atgcagcagg tacggataat tcacatgcac
     3241 tttcaggtcc tgcgtctgca cgcgcaggcg gctccacgtc agctcgagca cttcgccgtg
     3301 accgaagctg ttctgcagct tcagccgtgc gtcgccggaa aactggatct tgcccgtccg
     3361 gctgtcggga agaaaaccga cgacgccgtc gaattgtccc gcgcgctttt tgtcgaggta
     3421 caaagtgagc ttcgtgtatt tctccgtgaa aagaattgtg tacgattttg tcactttcgc
     3481 gaaagccagc tcgcgcagcc gtttggaaag cgcaaccagc ttgctttcgt cgtacgcgtc
     3541 gccgggcctg atgccgatgt aattgtacag gaagggccgg gaaattttca gggaaccatc
     3601 gacgtcgatg ctgtcgatct tcgtatagcg gtttttgttc agcgaaagct gcgcgctgat
     3661 cgtgttttca ttgtcgaacc tcacgctgtc gagctgcact gatgcgaacg ggtagccgtg
     3721 gttttcgcac cactccagga tattttcttc gactttcgcc acgtctttga accggatggc
     3781 gcggttgcgg aatgtttttt cgcggaagcc ggtggcggaa agtatctgct catctacatt
     3841 gcccttgccg atcctcgccc atttgtattt cacgccggga tcgaggtagg ctgttgcgga
     3901 agaagaatct tcacgcacgc tgtcgtatct cgctgcgatg tacgattcat tccacagcgt
     3961 cacgagaatg tcgttcatcg ttttcgtgcg ttcagctttg ctccggaatg ttttcgcgta
     4021 atcgatcttt ttcagcgcat gctcattccc gggttcgatg acgtcgaggg aaaacgtcgt
     4081 ctgggcggaa agcattccgc ggagaagcac gaatacaatg acgggaacgg gaagccgcat
     4141 ctgacgaagt tactgcggtg acgcaaaagg aagaagaata ttgtgtgcgg gccgtttttc
     4201 aggtgtacac acgcggacag ctttcaccgt ggctgaaggt gaatttcagt cccacacgaa
     4261 gctcccagca atgctggcgt atgtcgaaat ggtccgatgt gtaggaatgc gccaggtccg
     4321 gcacccacca cgcttccgtg aggaatttca ccggtccgta cgccacgaat tcaacgcctg
     4381 cgccgagcgc gccggtaacg tggaattttt tgaagctctg ctgcgagctg aaaacatatt
     4441 cgagccgcgg acccaccttc gcgtacggga tgatagaaac catttcgcgg cggatcatca
     4501 ggtagttatt ccacgccgca tattggttcg tatacttgtt cgtgacggtt cccgtcccga
     4561 ccgggttggt cgtctgtcct tcgaacttcg cgcctttcac gttgtactgc agctctgtta
     4621 cccagcggaa cgtgggatta tcgaagaact ccgcaaaaat ttccccgttg taacgaagca
     4681 ggtatttcga ttttgacttt gaatccgggc tgtaaaaatc ccagcgctgc cgtcctgccg
     4741 tggctccggc catgatgccg aatccctgga agaattgcgc tgcgcaagaa agcacgcttg
     4801 ccgaaaaaac cgtgagaagg atgatctttt tcatgtgatg tttgcctcga ctaatatata
     4861 acgcaaaagc aggcccggcg gttgcctgcc gggtaacttt taaaggggaa atgttaatta
     4921 aaaagtgaat ggggaaattt ttccggcttt agtcagtctt cgatacgttg ctttgcaact
     4981 actcagactg actaaagccc ggctcacagt tttcagcccg gcgctgatgt ttgctttgca
     5041 actactcaga ctgactaaag cccggctcac agttttcagt ccggcgctga tgtttgcttc
     5101 gcaactactc agactgacta ctgcccacct cacgcgctca ccgcttccgc cccggcgctg
     5161 atctttttca cgaggccctg cagcactttt cccggaccca cttcgatgaa attcgttgca
     5221 ccgtcagcca ccatgttctg cacggtttgt gtccagcgta cgggcgctgt cagctgcgcg
     5281 atgaggttct gctgtatttc tgccggatcg agcaccggtt tcgcgttcac attctggtaa
     5341 acggggcata ccggcgccga aaattttgtc gccctgatcg ccgcttccag ctccacgcgt
     5401 gccggctcca tcagcggaga atggaaggcg ccgcccacgg gcagcacgag cgcacgtttt
     5461 gcgccggccg ctttcagcag ctcgatcgct ttgttgattc ccgtcaccga gccggagatc
     5521 accagctggc cgggacagtt gtaattcgca gcaacgacaa catcgtccgt taccgaagcg
     5581 cagatctctt ccactttccc gtcgtccatg ccgagcacgg ctgccatcgt ggagggttgc
     5641 atctcgcatg ctttctgcat cgccatcgcg cgtttcgaaa caagaacgag cgcatcttcg
     5701 aacgcgagcg ttttgtttgc cacgagcgca gagaattcgc cgagcgaatg gcctgccacc
     5761 atatcaggat gaaaatcgcc gagcgttgca gcgagtgaaa cggaatgaag gaatatcgcg
     5821 ggctgcgtta ctttcgtctg cttcagctct tcgtctgttc cgctgaacat cgtgtcagtg
     5881 atacggaaac cgaggatttc attcgcacgc tccagcattt ctttcgcaac ggaattcgtt
     5941 tcgtacagct ctttcgccat tccgggaaac tgtgacccct gtccggggaa gatgtaggct
     6001 tttttcataa ggaaatgttt cgttgaatga agcgaagata acggatgcgc agaaaatgca
     6061 ggtcttgccg gtgaaatgtt gctgcggaat cccggtgtgc agattggcga attgcgcgtc
     6121 ctgataagtt cacaaaaaag ccccgcattg gtgcgaggct tttcgatggg agtaatgaat
     6181 tttacctgcg gctcccgagc aggcggagga tcgtcaggaa aaggttgatg aacgtgatgt
     6241 aaagcgtgag cgctccccag atcgcgagct tcgggcggga agtacccacc tgctgcccga
     6301 tcgtttttat ctgctgcatt ttgaaagcga ccagcccggt gaaaacgaca aggcacgcaa
     6361 tgtcgatgag ataggaaaac ccgctgctgt gcatgaacca gttgatgatc atcgagacaa
     6421 caatgccgaa cacgccgatc atgaggatgg aaccgaaacg tgtaaggtct gttttcgtcg
     6481 tgtacccgag gatcgccatg atgccgaacg tgcccgcagt gagcgcaaag gctttgacga
     6541 taagcagcgg atcgaatgcg agaaaaataa cgcagaggct gacgccgagc agcgccgaat
     6601 aaaggatgta aaggcttgcc accaatgccg tgctcattcg gtcgagcccg atgctgacag
     6661 ccattaccac ggcaatggga gagaacgtcg cgatccagaa cgcagccgtg ggcttcgcgg
     6721 ttgccgcgtc gaaaagaatg gaggtaagcg atggaatatt cgcgaatgca aaggcgatgc
     6781 ctgccgtgat aagcagcgcg ccgaacatcc acccgaaaac ctgcgccacg aaagcgcggg
     6841 tcgcctgctc ggtaatgtat tctttaccga tagtttcctc gttttgaaaa ttgttgtaca
     6901 tggttttatt gtttttaagg ttaatgcaat ttcgatccga tcagcttgat gaattccgac
     6961 cgcgtggttt cctgcaaaaa ttcacccgtg aacgccgatg ttgtcgtcac ggaattctgt
     7021 ttctgtatgc cgcgcatctg catacataaa tgctgcgctt cgataacgac acccacgccg
     7081 agcggcttca tcgtatcctg gatgcaatcg cggatctcgt tcgtgagacg ttcctgcacc
     7141 tgcaggcgtc gcgcgaaggc gtccaccacg cgggggattt tgctcagtcc caccacgcac
     7201 ccgttcggga tatacgccac gtgcgctttg ccgaagaacg gaagcatgtg gtgctcgcac
     7261 atggaatata cttcgatgtc tttcacgatc accatctggc tgtattcttc gcggaacatc
     7321 gcggaacgga gaatttccgc cgggttcaga tcgtagccgt gcgtgaggaa ctgcatcgct
     7381 ttcgccacgc gctccggcgt tttcgccagt ccttcccgtt cgggatcctc cccgatcttt
     7441 tccaggatca gcttgtactg cctggaaaga ctctcgatcg ccgacttgtt gtacttgtcg
     7501 atcttggtat atccgaattc ttcttccatt aaaagttctt tgttcaggtt ctgattaaat
     7561 attcacgcct catatctcac gtcccgcttc cgaaatattc cacataatta ttctccgtct
     7621 ccaccagctt cacgccatgc agctttgccc cggattccgc caccggtttt tccagctggt
     7681 tccagatggc gaccgcgata ttttccgtcg aggtcattac gccctccagg aaatccacat
     7741 ccaggttcag gttgcggtgg tccagctttt cagtgacgag cttccggatc atcccgctca
     7801 ggtctttcag gttcatcaca tagccgagag acggatcgat atttcccttt acggtaacaa
     7861 acagttcata attgtgcccg tgccagttcg gattggcgca tccgccgaaa atctccgcgt
     7921 tcttttcatc gctccattca gggcggaaaa gtttgtgggc ggcggtaaac ctttccctgc
     7981 gtgtgatgta gatcattttc attgcaaata tacgccaaaa acaaagggcg gcaggggcgc
     8041 agaacagcgt taaaaaatca aaaagtcttc tgcatcccgg ttgtgcagtc tcctgttttt
     8101 ccttttgaag attgatcccc gccttcctac cttcgttaaa tgaagatact cgtcgtttct
     8161 gccacctaca tggagatcgc accgctcgtt gcgaaaatga aattcaccgg cgaggcggga
     8221 ccacgcctgc gcgcttacga agccggaagg catcacgtag acgtgctcat ctccggtgtc
     8281 ggcatgacgg ctacggctta ctggctcggc cacgtttttg caaaggagaa atacgaagcc
     8341 gcgttcaatt tcggaacgtg cggcagcttc gatccttcgt tcgcaccggg aacggtggtg
     8401 caggtgatct ccgaccgcat ttcggaagcg ggtgcggaag acggcgataa atttctttcc
     8461 gtgagcgagc tcaacctgct cggcgaaaat gaatttccgt acaagtgggg acagcttgtc
     8521 aatcttcacc ctatgcagaa tccttcgctg tcctccctgc agcaggtaaa cgggatcacg
     8581 gtgaacacgg tccacggcgc ggaacattcg atccataaag tgaaggaacg ttacaagccg
     8641 cagacggaat cgatggaggg cgctgcgttc gcgtatgcct gcctcgttga aaacattccg
     8701 tatgcccagg tgcgcgcggt ttccaatatc gtcgaacgcc gtaaccgcga tgcgtggaaa
     8761 atggcggagg cgatcaggaa cctcaatgaa aaagcggcgg agatactcga caacgtctga
     8821 tcttcatata cgcgggtatt gccccgcgat catgcgtgtg aaatttacct gaaaccatcc
     8881 tggaagtgaa aatagctctc ggattttctc cctgccccaa cgactgcttc atcttcgatg
     8941 cgatcgtgaa caaacgtatc gacacgcagg gaatcgattt tgacatcgtg atggaggatg
     9001 tggaaacgct caaccgcaaa gcgttttctt cttcgatcga cgtgaccaag ctcagctacc
     9061 atgctttcgc gtggtgcgcg gaaaaatacg tactgcttga ttccggcagc gcactcggcc
     9121 gtaattgtgg tccgctgctg atctcgaagc gtgaaatttc catcgatgaa gtgaaaaacg
     9181 ggaagctgaa catcgctatt cccgggaaat atacgacggc gaatttcctg ctcggctacg
     9241 cattcccgga agcgaagaat aaaacggagc tcgtgttctc cgaaattgaa aaagcgctgc
     9301 tcgaggaaaa agcagatgca ggcctcatta tccacgagaa ccgtttcacc tatgaagcga
     9361 aaggactgcg gaagatcatc gacctcggcg agttctggga agaaaccacg aaagcgccca
     9421 ttccgctggg cggcattgcc atgcggcggg aattcccggc tgacgttcag aaaaaagtga
     9481 gcgcgctcat ccgcgaaagc gtggaatacg cgttcgcgca ccgggaagaa agcctgccgt
     9541 tcatccgcgc gcacgcgcag gaaatgagcg aggaagtgat gtacaagcac atcgacctgt
     9601 acgtgaacgg attttccgtc accctcggga cggaaggaag acgggcggtg gaaatcctgt
     9661 tcggcaaagc gctggagaaa aagatcattc ccccgttctc cggtgatctt ttcgtcgccc
     9721 gctgaatacg ggatgcgctg gtttttcttt tctaccttcg tgcggaatgc atttcctcca
     9781 cgacattctc agcgatccgg ccaaagcatt gctgatcatt ttcaatctcg tcatcatcga
     9841 aagcctgctt tccgtcgaca acgcagccgt actcgccacg atggtgatgg atcttccgaa
     9901 agagcagcgc gggaaagcct tgcgctacgg gattttcggc gcctacattt tccgcggcgc
     9961 cagtctcgtg cttgccgcgt ggctggtcca catctggtgg ctgaaagcgg cgggcggcgc
    10021 gtacctgctt tatctttccg tgaattattt cgcagccaaa gcaacgccgg aaaaagagga
    10081 cgatccgctg aataaatcgg aaagctggat ctataaaaaa acaaaaggat ttctcagcgc
    10141 gttctgggcc accgtcatcc tggtcgaggt gatggacatg gcgttttcca tcgacaatgt
    10201 ttttgccgcc gtggcgttca ccgataatat cctgctcatc tgcgccggtg tttttatcgg
    10261 catccttgcg atgcgtttcg tcgcgcaggg gtttgtgaag ctgatggaaa aatacgcgtt
    10321 cctcgaaacg tcggccttca ttgtcatttt cctcctcggg ctgaagcttt ccgttacgct
    10381 gtggacgcat ttcacgccat tgtctcctct cgcattgttc ctcgaaagtc atacggctga
    10441 cctgtacgtt tccatcggta cgatgacgat ctttcttttg ccattgctca cttctgcgct
    10501 tttcggatgg ccgaagcgtg aaaagtgagc ggaagttttt ctgaactttg ttcaactatg
    10561 aaggtaatca caggtgctgc gggattcatc ggttcctgcc tcgtttcaaa gctcaacgaa
    10621 gaaggagcgg aagacattat cctcgttgat gatttttcac gcgaggataa gaacaggaac
    10681 ctcgaaggga aaatattcct gaaaaaaatt caccgcgacg atttcccgaa gtggatgcaa
    10741 gagcacggcg gcaagctgga tgcggttttc cacatcgggg cgcggaccga cacgacggaa
    10801 ttcgacaaat ccgttttcga ccggctgaac ctgaattatt cgaaagcgat ctgggagctt
    10861 tgctgcaggt tcgatatccc gctggtgtat gcgtcttccg cagccacata cggactcggc
    10921 gaattcggtt acaaagatga tcatgctgtt gtcgaaaaac tgaaaccgct gaacccgtac
    10981 ggcgagtcga agaatgattt cgaccgctgg gtactgaaac agaagcatca tccgccgcac
    11041 tggtacggac tcaaattttt caatgtgtac ggacccaacg aatatcacaa gggaagaatg
    11101 gcgtccgtta tttttcatgc gttcgggcag atatccgaaa ccggcgccat gcggcttttc
    11161 cgttcgcaca acgcgaagta taaagacggt gggcagctcc gtgatttcgt ttacgtgaag
    11221 gatgtcacgg aagtgatcga tttcctcgct gaccagtatc ccgaaagcgg gatctataac
    11281 ctcggtacgg gaaaggcgcg cacatttctc gatcttgcac ggagcacgtt cagcgcaatg
    11341 ggaagagaag aacggaccac gttcatcgat acacccgaag acatccgcga caagtaccag
    11401 tattttacag aagcggacat gtcaaagctg aaaaaagcag gatacaaaaa tgaatttgct
    11461 tcgctcgagg acggcgtgcg ggattacgtg cagaactacc tggcagggca taattattta
    11521 tgaacctttc cggcagtggt aacatgagat ccatctttta caccgttacg cttgccgctt
    11581 tgctctcttc gtgtggtaat gcggaaaaag atccgcggcg cgattcgatg ttccgtgatt
    11641 ccgttgccgc ggcgaaaaag gatccggcgc cttccgcaaa agagaacaat gccggggcca
    11701 tacccgtagc ggatgtaatg ccgtcgacct gcgacagctc gatttacgaa gattatctcc
    11761 cgtcttccct gcaggatttt cttgaaaagg atttcagcgg ctggaaactt gtatcgcctt
    11821 ctttgtggag cgggtactgg ttcgataagt acaaaacgga cagctcgctt gtcgatctgt
    11881 tcccggcgga tttcaactgc gatgacaaaa cggattatgc attgctgatg tataacgcgg
    11941 aacggaaaga gaccggcctc tggattttca ttgcgggaaa ggcaggtttt tcagccgaga
    12001 aagcggatgt gtgggaaaat cagccggaag ccgatctcgg tttgtatgca attcagcgtg
    12061 caccggaccg cgcagtgacg gacgtgtatg aaaacaaaac ggtgaagccc gattgcgatg
    12121 cgatcgattt tgtgaaattc gaatcggcgt cgagagtgta ttaccgcgcg aacggaaaat
    12181 acctgtatgt gcagacgagc gactgaataa aaaaaacagc acccgtccgg atgctgttcc
    12241 cgtgttttgt tacctggtta tttcttgcgg cctttctggc ctttctgctg ctgctggagc
    12301 tcttccatgc gcttgcgctg cgcctgctcg aggcgctgca tcagtccggg ttttttggtc
    12361 acctgcttct tcttgttctc ctggatcttc gcgtggatcg ctttttcatc cacgaacctg
    12421 cgcatgagga acatttgtcc gaacgtgatg atgttgctga cgaaataata atagctcagc
    12481 gctgcggaat acgaattcag cgcaaagaag aacacgaccg gcatcaggta aacgagccat
    12541 ttcatgcccg gcatttgcgt gcttcccggt gtcagcatct gctggttcat ccacgtgtag
    12601 agaatggtcg atgccgtcat cagcagcgcg aaaaggctta cgtgatcacc cagcagccag
    12661 atgctgtggc tgaaattcca ggcggaatcg taagtcgaca ggtccttcgc ccaccacagt
    12721 ccctgctggc ggatctcgaa cgacgcgggg aaaaggcgga tgagcgagaa aaggatcggg
    12781 atctgcagca acagcggaac gcagccggat gccgggttgg cgcccgcttt tttgtacagt
    12841 tccatcgtgg cctgctgttt tttcagctgg tcgccgccct tgtatttttc attgatctcg
    12901 tcgatctccg gtttcagcac gcgcatttt
//
LOCUS       DASTSF010000434         2150 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_596366,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000434 DASTSF010000000
VERSION     DASTSF010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2150
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_596366"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..201)
                     /locus_tag="VFU15_12565"
     CDS             complement(<1..201)
                     /locus_tag="VFU15_12565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718666.1"
                     /translation="MNNRKLLLLFALLSLLVLKGFAQAPPQGINYQAVARNASGAELT
                     NTTLSVRISIYSDAAATQLVFQE"
     gene            complement(213..>2150)
                     /locus_tag="VFU15_12570"
     CDS             complement(213..>2150)
                     /locus_tag="VFU15_12570"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HEU4718667.1"
                     /translation="AIVPEFGPDWDTTTLVPVTLPARQSNAGAWIWTISPTPDGPHHG
                     FFFDRNSNGNPADNTGDQGNVMSSWTGCWRIRTKPQCTTNDLSVNINLYSDDQTGTGV
                     PGFICSPYDVLHSECASSCCHGLYVNISQPTNCASNNASVVAAGYQVTGTDTFNYYLV
                     NQYHDTIQSYPSITGAVTFANLSAGQYIVSAVNLSNGNCEAFQTLTIHPAMQASVMQT
                     AMGCDSTSSGTAYAVVSGGLSPYTYNWLNVPAANQNDSVAIGLPDGWIHVQISDAAGC
                     IVTDSVFIQSIPAADPSFSYNHVPYCSNTDTIHVTTLPASPGGVFNLVSPLTAGITVD
                     TGNGDIFLHNTTFATPFWVYVSYSVSNGCSASYTDSVQVIAVPSPPVASTPSTISYCV
                     GSSLPVLGINPNANYIPFWVNVQDNSTALGYSYTPPAGSITAPGQYYYTAFYYSSLSG
                     GCASLPTLFLINAALPPVIQVSGDTTICANEVAFMNASGCSSCTYNWSPAPTGSSSTS
                     PSASTSPSSSTTYTVTATDASGCADMQTVNVVIDQSGACGGGSSAVSGEIHPWGGITP
                     NGDGNNDTWVIDGIDAFPDAIVIIYNRWGMEVWRKSGYDNTNVYWSGEAKDNSRLPDG
                     TYYYIIQLDDSSLHGWVELSR"
BASE COUNT          528 a          563 c          597 g          462 t
ORIGIN      
        1 ttcctggaac acgagctgcg tcgccgccgc atcgctgtag atggaaatgc gcaccgagag
       61 agtcgtgttc gtcagctccg cgcccgatgc gttccgcgcc accgcctggt aattgatgcc
      121 ctgcggggga gcctgcgcaa atcctttcag cacaagtaaa gacagaagag caaacagcaa
      181 tagtaattta cggttgttca ttttcatagt gtttatcttg acagctcgac ccatccgtgc
      241 aaggaagaat catccagctg tattatgtaa taataggttc cgtcaggcag gcgcgaattg
      301 tccttcgctt ctcccgacca gtaaacattc gtgttgtcgt aaccgctttt acgccatacc
      361 tccattcccc aacgattata gataatgaca atagcatccg gaaatgcatc gattccatca
      421 ataacccagg tatcattgtt tccatctccg ttcggcgtga tcccgcccca gggatggatt
      481 tcaccggaaa cagcagatga tccgccgccg caggcgcccg actgatcgat cacgacattc
      541 accgtctgca tatccgcgca gcccgatgca tccgtagccg taacggtata cgttgtcgat
      601 gacgaaggcg acgtggatgc ggaaggagat gtggacgagc ttcctgtcgg cgcgggcgac
      661 cagttgtatg tgcaggagga acaacccgac gcattcatga acgcaacttc attcgcgcag
      721 atcgtcgtgt cgccggaaac ctgtatcacc gggggaagcg cggcgttgat aaggaacaac
      781 gtcggaagac ttgcacatcc gccgctgagc gaagagtaat aaaatgccgt ataataatac
      841 tggcccggag ctgtgatgga accggccggc ggagtatacg aatacccgag cgccgtggaa
      901 ttatcctgca cattcaccca gaacggaatg tagttcgcat tgggattgat ccccaaaacg
      961 ggcaggctcg atccgacgca ataactgatc gtggaaggag tggacgcaac cggcggagaa
     1021 ggaacagcta tcacctggac agaatctgtg tacgatgcac tgcagccgtt tgacaccgaa
     1081 tagctgacat acacccagaa aggagtagcg aacgttgtat tgtgcaggaa aatatcgcca
     1141 ttgcccgtat ctacggtaat accggcagtt agcggagaaa cgagattgaa tacacctccc
     1201 ggggaagcag gtaatgttgt tacatgaata gtgtcggtgt tgctgcagta aggaacatgg
     1261 ttgtatgaaa aagacggatc ggcggcagga atggattgaa tgaaaacaga atccgtcacg
     1321 atgcagcctg ctgcgtccga gatctgcacg tggatccagc catccggcaa cccgatggcg
     1381 acagaatcat tctggttggc ggccggcaca ttcagccagt tgtaagtgta aggtgaaagt
     1441 ccgccggaaa caaccgcgta agccgttccc gaacttgtgc tgtcgcatcc catcgctgtc
     1501 tgcatgacgg atgcctgcat cgccgggtgg atcgtcaggg tctggaacgc ctcgcagttt
     1561 ccattggaaa gattcaccgc gctgacgatg tattgtcctg cagaaagatt ggcgaatgtc
     1621 accgcaccgg taatgctcgg gtacgactgg atcgtgtcat gatactggtt gacgagataa
     1681 tagttgaatg tatccgtacc tgttacctgg taaccggcag caacaacgga agcgttattc
     1741 gacgcgcaat tggtcggctg gctgatattc acatacaatc cgtgacaaca gcttgacgcg
     1801 cattcggaat gcagcacatc gtaaggtgag cagatgaaac cgggaacacc tgttccggtc
     1861 tggtcatcgc tgtacaggtt gatattcacc gaaagatcat tggtcgtgca ttgcggtttc
     1921 gtgcggatcc gccagcagcc ggtccacgag gacatcacat ttccctggtc gccggtattg
     1981 tcagcgggat ttccgttgct gttccggtcg aagaaaaaac cgtgatgcgg accatccggc
     2041 gtcggggaga tcgtccatat ccacgcaccg gcattcgact gccgcgccgg cagcgtaacc
     2101 gggacaagtg tcgtcgtgtc ccagtcgggc ccgaattccg ggacgatcgc
//
LOCUS       DASTSF010000435         2575 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_545910,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000435 DASTSF010000000
VERSION     DASTSF010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2575
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_545910"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1211
                     /locus_tag="VFU15_12575"
     CDS             <1..1211
                     /locus_tag="VFU15_12575"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HEU4718668.1"
                     /translation="SGAFVPNNTALNGTYIPSASDSLAGSVMLILTSTNTGSCAATSD
                     TMMVFISYPAVVNAGPDQSVCSVGAVVSLNGNVSGGTTTGIWSAAGSGTFTPSNTTLG
                     ATYTPSAADIAAGSVTLYLTSTGNGICAAREDSMVVTIVPAAVVNAGNDIVVCGSNTD
                     VPLNGTITGITTTGIWTTGGSGIFNPNATTLNANYALSNADVLGGSVTLVLTSTNNNI
                     CPSVSDTTVITIEPLPVAAFSSVSGDSLNVDFTDQSSGAVSWYWTFGDGGTSTQQNPQ
                     YNYPSSGSYQVVLVITSAGGCSDTATSNVNANEATVQPVAVPTGFSPNSDAHNDVLHV
                     LGGPFSELDFKIYNEWGNLIFSTQDPNGGWDGTYKGKPQPGGIYVYTVTGKTIDGKDV
                     NLSGNVTLIR"
     gene            1253..2248
                     /locus_tag="VFU15_12580"
     CDS             1253..2248
                     /locus_tag="VFU15_12580"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HEU4718669.1"
                     /translation="MTRKILFAAALLFAGQRIHAQDFHLSQYDAMPLYYNPAQTGMYF
                     GSSDDMKFRLCGSYRSQWQKLQGKPYSSVGISYDMPIQRWGVGVLMMDHIAGTSNFGT
                     FQFLLSGAYRITSDDSKKHFLSAGIQLGLFQKRFAYDNLLFESQYTPVDGLNPYLPNE
                     EAFDRLGIIRFDANVGIYYKYLDPNRKFDPAFGFSIYHLTTPNESFTGEKARLPMRFN
                     FTGACDIHANEDFTITPNVLFMYQRAAMEINAGLMFGYIMPDSPYDLLAGASYRYKDG
                     VVVHLGMKQGNNIFRVSYDIVTSPLYRFSGRRGGFEMGVIYSGAPKNGSHARANL"
     gene            2290..>2575
                     /locus_tag="VFU15_12585"
     CDS             2290..>2575
                     /locus_tag="VFU15_12585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718670.1"
                     /translation="MKKILVLFLLLSAFGSNAPASPVSLGEFLYTVPLKKSVTPAYAQ
                     TQWWHEYMEPSENEAFFGPLLDRIDSGLVDVYSPEFPFDKKISPVDVKTLL"
BASE COUNT          622 a          741 c          636 g          576 t
ORIGIN      
        1 gcagcggggc attcgttccg aacaacactg cgctcaacgg aacttatatt ccgagcgcaa
       61 gcgattcact tgccggcagc gtgatgctta tccttacttc caccaacacc ggctcctgtg
      121 ccgccaccag cgacacgatg atggtgttca tctcgtatcc ggccgttgtg aatgcaggac
      181 cggaccaaag cgtctgctcc gtcggtgccg tggtttccct gaacggaaac gtttccggtg
      241 gaacaacaac cggaatatgg tcagctgccg gaagcggaac atttacgccg tcgaacacta
      301 cgcttggcgc aacctatacg ccctctgcgg cggacatcgc tgcaggaagc gtcacactct
      361 atctcacgtc gaccggcaac ggaatttgcg ctgcacgcga agattcgatg gtggtgacga
      421 tcgttcccgc agctgtggtg aatgcaggaa atgacatcgt ggtgtgcgga agcaatacgg
      481 atgttccgct caacggaact atcaccggga tcacgacgac cggtatctgg accacaggcg
      541 gaagcgggat attcaacccg aacgcgacaa cgctcaatgc aaattatgcg ctcagcaatg
      601 ctgacgtact gggcggaagc gtgacacttg tgcttacatc cacgaacaac aacatctgtc
      661 cgtcggtaag cgatacaacg gtcatcacga tcgagccgct tcccgttgct gcattcagca
      721 gcgtctccgg cgattcgctc aatgtggatt tcacggatca gtcgtccggt gccgtcagct
      781 ggtactggac attcggtgac ggcggaacat caacgcagca aaacccgcaa tacaattatc
      841 cgtcctccgg cagctaccag gtggtgctcg tcatcacgag cgccggcggc tgttccgaca
      901 cggccacatc gaacgtcaat gccaatgaag caaccgtgca gcctgtcgcc gtacccaccg
      961 gcttctcacc gaacagcgat gcacataacg acgtgctgca tgtgctgggc ggacctttca
     1021 gcgaacttga cttcaagatc tacaacgagt ggggaaacct catcttctcc acgcaggatc
     1081 cgaacggcgg atgggacgga acctataaag gaaaaccgca gcccggtgga atttacgtgt
     1141 acaccgtaac aggaaaaacc attgacggga aggatgtgaa tctctccggc aacgtaacac
     1201 tcatccgtta accgaaaagc agaacaacat gaaaagaaaa aacatatttc ccatgacacg
     1261 gaaaattctc ttcgccgcag cgctgctttt tgcaggacaa cgcatacatg cccaggattt
     1321 tcacctttcc cagtatgatg cgatgccgct gtattacaac cctgcacaga ccgggatgta
     1381 tttcggcagc agcgatgaca tgaaattccg tctctgcgga tcgtaccgtt cgcaatggca
     1441 gaagctgcag ggaaaaccgt attcatccgt cggcatttcc tacgacatgc ccatccagcg
     1501 atggggcgtt ggcgtgctga tgatggacca tattgcagga acatccaatt tcgggacatt
     1561 ccagttcctg ctttccggcg cttaccgcat caccagcgac gacagcaaga agcatttcct
     1621 ttctgcagga attcagctcg gccttttcca gaaacggttt gcgtacgaca accttctctt
     1681 cgaaagccag tacactcctg tcgatggcct taatccttac ctgccgaatg aagaagcatt
     1741 cgaccgcctc ggcattatcc gcttcgatgc aaacgttggc atttattaca aatacctcga
     1801 tccgaaccgg aagttcgacc cggcattcgg tttttcgatc taccatctca cgacgccgaa
     1861 cgaatcgttc acgggagaaa aagcgcgcct gcccatgcgt ttcaatttta ccggggcgtg
     1921 cgatattcat gcgaatgaag atttcacgat cacaccgaac gttctgttca tgtaccagcg
     1981 tgcggcaatg gagatcaacg caggactcat gttcggatac atcatgccgg attccccgta
     2041 tgacctgctg gccggcgcca gctaccgtta caaggacggc gttgtcgtcc acctcgggat
     2101 gaagcaggga aataatattt tccgggtgag ctatgacatc gtcaccagcc cgctctaccg
     2161 tttcagtggc cgccgcggag gttttgaaat gggcgtcatc tattccggcg caccgaagaa
     2221 cggctcgcac gcgcgtgcaa acctctgacc gtacgttcgc attaaggctt tttctatttt
     2281 agtgaccgga tgaaaaaaat tctggtcctt tttcttttgc tgtccgcttt cggaagcaat
     2341 gcgcccgcct ctcccgtttc actcggtgaa ttcctttaca ccgttccgct caaaaaatcc
     2401 gtaacacctg cgtatgcaca aacgcaatgg tggcacgaat acatggagcc ttcggaaaat
     2461 gaagcctttt tcggtccgct cctggaccgg atcgacagcg gactcgtcga tgtttattct
     2521 cccgaattcc ctttcgataa aaaaatttca cctgtcgatg tgaaaacact tctcc
//
LOCUS       DASTSF010000436        35325 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1274032,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000436 DASTSF010000000
VERSION     DASTSF010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 35325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35325
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1274032"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..132)
                     /locus_tag="VFU15_12590"
     CDS             complement(<1..132)
                     /locus_tag="VFU15_12590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013750666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU4718671.1"
                     /translation="MSLLREYDVIVVGAGHAGCEAAAAAANLGSSVLLVTMNMQTIAQ
                     "
     gene            complement(216..680)
                     /locus_tag="VFU15_12595"
     CDS             complement(216..680)
                     /locus_tag="VFU15_12595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718672.1"
                     /translation="MKKLFTIGFISLGLMSFAQTSKPTTATSGTTGASATQSTLTPKE
                     KILCRTWVLSKTEVFGVEKGPQDDQKNDQLVVQENGRYRLIYNGVAEGGTWVLDKGGI
                     WLTLTSDAGVVRKFKIVSMTEQQLQVDYKDSDDVHNNIYYVPVNADGTNKQK"
     gene            complement(691..1131)
                     /gene="ybeY"
                     /locus_tag="VFU15_12600"
     CDS             complement(691..1131)
                     /gene="ybeY"
                     /locus_tag="VFU15_12600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011586841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEU4718673.1"
                     /translation="MNSPSSEIYFFPEDVKLTLRDKGKVRAWLIGIAQNHRYDILSLN
                     YVFVTDRKLLRINTEYLGHDTLTDIITFDLRVGREKKISAEIYISVARVRENAKKYGC
                     TFRDELHRVMAHGLLHLVGFRDKTSAEKERMRRQEEKALALRTF"
     gene            complement(1131..1541)
                     /locus_tag="VFU15_12605"
     CDS             complement(1131..1541)
                     /locus_tag="VFU15_12605"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017730992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU4718674.1"
                     /translation="MIRDQKISISSVPGNIGIIEKLINEICTACNVSEDYYGNILVAV
                     TEAVNNAMQHGNRYDPQKNVNLNVSATDNALMFSISDQGPGFDYNNLPDPTNPENLEK
                     PNGRGVFLMRHLADKVEFSDNGKSVSLEFVISPN"
     gene            complement(1556..4909)
                     /locus_tag="VFU15_12610"
     CDS             complement(1556..4909)
                     /locus_tag="VFU15_12610"
                     /inference="COORDINATES: protein motif:HMM:NF025152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4175 family protein"
                     /protein_id="HEU4718675.1"
                     /translation="MENTTGSYELLLGKLDEFIRKYYKNMLIRGMLYAVGILLAGYLV
                     FTVSEYFGHFDTSVRTVLFYSFIGATLFVLGRFVALPLFKLNRIGKIISHEQAAEIIG
                     KHFTNVQDKLLNVLQLKKSSAADSGASRALIEASISQKISELRPVPFTSAIDLRENRK
                     YARYAVIPLLVMLVIVFTNAGILSEGTRRLVNHGEDYETQAPFRFEIAQDLKGVENED
                     FPLHVKLTGSEIPETVYMLVDGNEYRMNRDNTINFSYVFRNLQKTQTFRLSADGFTSK
                     EYTLEVLPNPIVLNFDVDLHYPKYIGKKDETLKNTGDLVIPAGTSVTWRFNTRATKNF
                     LFAFSDTSYSLKPTGENQFAFTQRFLESRNYSLHTANEFLKSRDSIRYSVNVIPDLYP
                     SISVNEKNDSVSRLRMYFTGEVKDDYGFTKLAFVYHFLSHKDSTGKELPDKTQIVNLP
                     VSDKLVRDQFYYYWDLSRLNIGMGDQVEYYFEVWDNDGVHGAKSTRSQKMIYHAPTKD
                     QLNQQNDANNKNTETELEESLAESKALQKDIDDLYKKTMEQKSLTWEDKKKLEDLQNR
                     QQALQTKIDNLKQQNAQNMQQQSEFSPNDPDLQQKQSEIQNLFDQLKTDDMKRMIQQL
                     QQLLQNADKNKTQAALDSMKQENTDAQKDLDRTLNLFRDMLVQQKLDQAIQDLNQMQK
                     QQDSLGNLSAQKNLSKQQKEDIKKQQDSLNSKFQQLRQNLDQIQDENSKLDSPHDIPN
                     TDLQEMEIQQQQREGSQNLNEGDSKKGSQNQKNAAQQMQDLSAQLQAAQKKMDEEENA
                     EDMQAIRQLLSNLIQLSFDQEALMAKVQNTDVNDPQYPALAREQKKLSDDSKMIEDSL
                     LALSKRNPQISPLVNREVTNINQNMDRAMVALGDRNTGEARYRQQIAMTSINNLALML
                     NESLENMMQQANQQNKSQCSGGKCNKPGNGKKNKPGMSSLRQMQQSLNQQMQQLSQDP
                     NGQNAEQLAKLAAQQEYIRMMLQQAQQDNKDPNGGGVDPGGQVQQKMEQTETDLVNKN
                     INAQTLQRQQDILDKLLQFEKAEQQRDMDNQRQANEAKNQQQSNPNGFSEYNRQKQKE
                     AELLRTVPPALSPFYKNKVNNYFNGVQQK"
     gene            5088..5255
                     /locus_tag="VFU15_12615"
     CDS             5088..5255
                     /locus_tag="VFU15_12615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718676.1"
                     /translation="MLIAAIAFMTTIAVESCHQACKHAQKTNAALATGTYRFGMKKHS
                     KYKRMNRKSAG"
     gene            complement(5311..6195)
                     /locus_tag="VFU15_12620"
     CDS             complement(5311..6195)
                     /locus_tag="VFU15_12620"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016194255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU4718677.1"
                     /translation="MLLLSPSRWKDYELIDSGNFLKLERFGKYITIRPEPQAVWDPAQ
                     NEKEWEKIADVKFVPKSSSSGEWKKLKRDMPDRWNISYEFEDGKKISLRLALTAFKHV
                     GVFPEQAVNWDYIYSSVRKMSAAQKPDQAPKFLNLFAYTGGASLAARAAGADVTHVDS
                     VKQVVTWANENMSLSGLDNIRWLVEDALKFVKRGQRRGNRYNGIILDPPAYGHGPNGE
                     KWKLEENINEMVKAVVELLDEKDHFLILNAYSLGFSAMILENLFSGRKVETGELFLRA
                     KHGSVLPLGVFGRIGHHV"
     gene            6277..7818
                     /gene="gltX"
                     /locus_tag="VFU15_12625"
     CDS             6277..7818
                     /gene="gltX"
                     /locus_tag="VFU15_12625"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015813707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEU4718678.1"
                     /translation="MENRRTRVRFAPSPTGPLHMGGVRTALYNYLIAKKAGGDFILRI
                     EDTDQERFVPGAEEYIIEALKWCGIEPNEGIGFGDGPFAPYRQSERKEKGIYNQYVQQ
                     LIDSGNAYYAFDTKEELDAQRERFEKMSKTFSYNAVTRQELKNSLSLSEDEVKKRISS
                     GEHYVVRMKIPRNEEVRFHDLVRGWVVVHSSQLDDKVLFKSDGMPTYHLANVVDDYLM
                     QITQVIRGEEWLPSAPLHVLLYRAFGWENVMPEFAHLPLLLRPDGNGKLSKRDGDRLG
                     FPVFPLSGELLDVKTKKPEKFSGYREAGYFPEAFINMLALLGWHGSGNQELYSKDELI
                     HEFSLERVSKSGAKFDAEKTKWFNQQYLRKHSDAGLAQLLIAGGQLPAADPGFITEVC
                     RLMKEKVNFVQEIYEQGKFFFDDPSAYDDGVIKKRWNEGAKNFITALINSFSAVNSWT
                     AADCEALFKKTAEETGTNPGHVMQLFRVLVSGAGGGPVLFEMTALLGKETIVRRLKTA
                     LEKLA"
     gene            7825..8175
                     /gene="folB"
                     /locus_tag="VFU15_12630"
     CDS             7825..8175
                     /gene="folB"
                     /locus_tag="VFU15_12630"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010250398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEU4718679.1"
                     /translation="MGIINVNGIRVYAYHGCLPEETKIGGEYVVDVSIETDFSKAAES
                     DDLSETVDYCTVNEIVRTEMTLPSKLIEHVAKRIADHLRKKFPAVKLFRVTVTKINPP
                     MNGAVDSVSVVVEG"
     gene            complement(8197..9816)
                     /locus_tag="VFU15_12635"
     CDS             complement(8197..9816)
                     /locus_tag="VFU15_12635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718680.1"
                     /translation="MFRKRYFPKRSFGLFLAAFVVLCLLYNYPYIYRYGPRGHHTWRQ
                     ADCYSMATNYYFGHRSFLDPQVHFCSPAGPQETVSEMPLLNYSVAQLWRIFGYHPWVY
                     RAVQNIILFLGLLCLFRIFELSLRNTWWALGLSLLFFTTPLLSTYGISFIADASAMCI
                     AICGGWFWFRYHRWNRWTDLVFAALLTTIAGWMKITSLYTLGIVCSLALAGLLPGKKR
                     MAVFRRPAAVMISAGVVLLLVAAWYVYADHYNKTHDEGVFLVGILPVWDMTWAEIVLT
                     SRSLVNDLLPVYFNRPVLFFLSGVMLWMFLDWRRMGKVSVFILSVSALIVLLYLLLFF
                     QVFTVHDYYLVNMLPFVAVLLLVAARYVRKTAASLFHDRRVQVVSGVVLLFSAWNCAM
                     ITRIRYSSIDPFAETSVFISQEEYSKWHNFDHTYEKDMINLQEARPVFDSLKITRDDI
                     VISIPDPSPDYSLVMMDRKGYTDCSYSSLGNRGDRIHAFISYGANYLVLNCEGMQYDP
                     DILPYTKFEMGRFRQARIYDLRPYADSIRAK"
     gene            9942..10454
                     /locus_tag="VFU15_12640"
     CDS             9942..10454
                     /locus_tag="VFU15_12640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718681.1"
                     /translation="MKYFFPVIFLFLISCGGKGNLQLPGVSIEKNAKLEAMQKFHIAA
                     LKAQDNILLWVAIPGSDESKVLLSAFISGGHTSPETALRSSFRNDTVHRAKIDTLISH
                     YGTLLNAYKDVTSSLNSIADYNDPARMLPAQAMVYDNDGAVRMLFRRFENELTALEQE
                     SEEPAAVVIR"
     gene            10520..10590
                     /locus_tag="VFU15_12645"
     tRNA            10520..10590
                     /locus_tag="VFU15_12645"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10552..10554,aa:Cys,seq:gca)
     gene            complement(10714..11637)
                     /locus_tag="VFU15_12650"
     CDS             complement(10714..11637)
                     /locus_tag="VFU15_12650"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEU4718682.1"
                     /translation="MEQETKTATFERRGRELIHTRLLNAPRELVWEVWTDPEHIKNWW
                     GPNGFSLTHKNMEVKPGGEWRFMFHGPDGRNYPNKIVYVEVVKPERIVYEHSDDGDTE
                     PVNFRSEITFEARGDKTFLTMRGIFPSEEELDRLEKEHGAIEGGKQTLNRLGEYLSKM
                     YTVMQPGAFTIERIFNASVARVWKAITDKDEMKKWYFDLPEFRAEKGCEFRFYGGKDP
                     GRPYLHICKVTEVVAMEKLTYSWRYDGYSGNSLVTFELFPEEGKTRLRLMHSGLLSFP
                     ADNPDFDPENFAGGWTYLVGTALAEFLAKQN"
     gene            complement(11639..12139)
                     /locus_tag="VFU15_12655"
     CDS             complement(11639..12139)
                     /locus_tag="VFU15_12655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015813721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEU4718683.1"
                     /translation="MKTIFTKDLPGKKIFVTREFAAPLEMTWTAWTDKDILDKWWAPK
                     PWKAETKEMDFREGGRWLYCMVGPNGERHWARADYMKIIPHKFFHGKDSFCDENGTVF
                     PQPPGMDWDVEFGKTASGTKVTVEITFASEKDLATIIEMGFEQGFTMAHGNLDEYFVA
                     QTKTTH"
     gene            complement(12211..12588)
                     /locus_tag="VFU15_12660"
     CDS             complement(12211..12588)
                     /locus_tag="VFU15_12660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019988283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEU4718684.1"
                     /translation="MKKTNIIYWISTGLFAAVMLFSAVPDVLNTADAVSFMSKLGYPS
                     YFTPFIGLMKVLGVAALLVPGFPRVKEWAYAGLAFDLLGAVYSVLAVGTPPLMVLPMA
                     LFIAPGVVSYIFWRKKTGAVHAQ"
     gene            complement(12645..12989)
                     /locus_tag="VFU15_12665"
     CDS             complement(12645..12989)
                     /locus_tag="VFU15_12665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEU4718685.1"
                     /translation="MRRDVFQAIADPTRREILHMIAHQPLNVNSVAGNFDVSRTAVYK
                     HLKILTECGLVVVKQQGRERYCEAKLSKLNEVSDWVNQYREFWEKKLDALEAYLEELQ
                     SKKKKNGRKKRK"
     gene            13306..14418
                     /locus_tag="VFU15_12670"
     CDS             13306..14418
                     /locus_tag="VFU15_12670"
                     /inference="COORDINATES: protein motif:HMM:NF025007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin-like domain-containing protein"
                     /protein_id="HEU4718686.1"
                     /translation="MAHNTFMEEPCRDFRRVFTAGQRFRYGIYQLSDHFFGRKKFFNK
                     SRQKFFRSLAAHLEKQGEGKIMPVERRKDLSLEEFKSHYVRKGIPVVLEGAAKDWACV
                     QKWSLEYFRQLHGKDEIVLVDQGKKEYPYELTTLADVIDNIRGGGGKYYRFYPLLTRH
                     PEHINDFDYKFLRERRTSPTWFDAFQVFIGGENSYTCLHNANQSNLFVQVYGEKKWVL
                     YSHYYSPVIDPDPVRNVYRNAPSKREDGPFNPFEPDFSPPYELYRYLDGYSVHLKPGD
                     ILWNPPFYWHAVKNTADSIGVGYRWLSPFYSFRVSPLYMLLDCFATNPPMWKAYSLYR
                     KDLNELHLAEVKQLEEAKKKIAEHEAKNRPVTGLSA"
     gene            complement(14408..15991)
                     /locus_tag="VFU15_12675"
     CDS             complement(14408..15991)
                     /locus_tag="VFU15_12675"
                     /inference="COORDINATES: protein motif:HMM:NF039201.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEU4718687.1"
                     /translation="MKKLLTLLAVVFTAHILSAQTKINEAIGGPGQDEAIYAETTTDG
                     GYVIVGWTTSYGVWGWDIYLVKTDSLGAIEWTKTYGANGDEVDCFVQQTTDGGFIITA
                     RSNSFGAGSTDVYLIKTDPAGNLQWTKTIGGSDWDEGHAVLQTPDGGYIHTGYTRSFG
                     AGGDDVYLMKNDSAGNLLWTKTYGGAGDEHGHFIQQTIDGGYIIGGETNSFGAGGWDY
                     YLVKFDVSGNLQWSKTYGSPGDDHGWSVQQVPSDSGYIIGGFTNGYGTEGEDALLIRT
                     DAGGNVTWSKTYGGASADEAYAVHLCSDGGFIIAGNTKSFGAGNTDVYLVKTDRNGNA
                     QWSKTYGGSGNDACQSVQQTNDGGFLIAGYTTSFGMGGADFYMIKTDSLGNSSGCNET
                     SPLTNVFSQAIVAVNAPTLTGSGGICGTPVSLQNSGGLVSQCVPTGINELTSKSAFGI
                     SPNPNNGTASVSYSLDAGKTGCVRIYDMTGKCVSETMIQSAATTASLDLSQLDDGIYF
                     CRMFVDGVATGGQKIMITR"
     gene            complement(16142..16792)
                     /locus_tag="VFU15_12680"
     CDS             complement(16142..16792)
                     /locus_tag="VFU15_12680"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU4718688.1"
                     /translation="MKKILVIEDNPVLRENIAEILGVGNYEVIAAGNGKTGLGYAAKN
                     QPDLILCDISMPEMDGFEVRRVLGNRMETAGIPFIFLSANADKESRRTGMSLGADDYI
                     TKPFDAETLLEIVRVRIERREQTRRQTEKELLHYVSALEEMLEMVSHRVRKPLCTCLG
                     LIQLLEKGDGGILNSSELQKVITHVRSSTGELDDFTRELTTFMQEERKRILGKLDG"
     gene            complement(16990..18276)
                     /locus_tag="VFU15_12685"
     CDS             complement(16990..18276)
                     /locus_tag="VFU15_12685"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEU4718689.1"
                     /translation="MSFLSAITEFRQPQIMRALAGFFPGAPVQEKFVPGTDCIPDRPD
                     EIRAELLSHLEIFNQSALLTISDLKGNIIHANEKFCQVAGYSMEEVMGKPHSIIRHPD
                     MPSSVFKEMWRTIGSGKVWQGEIKNRAKDGSAYWVMATIAPVMGKNGKPERYISVRYD
                     ITKQKRVEEELREAQKRMDRELMESIGYAKYIHGTILNNNDERDEAEEDSFLIYKACR
                     MISGDFYKVRQDKKKLTFAVGDSTGHGVSASYISVLALSILNRLADAGCRNPAETLRK
                     MNTELNRITHSDRKKHLLESADLMIGCIDKKTLKMTYASARMRGVVVRNGEVIALEKD
                     KCTIGDKGAHEFEISSRTINLQKGDRLYIYSDGFTDQLGSAENKRFGQKRVLSLLKEV
                     CQRPMHVQKQLIEEQLLAWQDENGQTDDITVLAFRI"
     gene            complement(18485..20284)
                     /locus_tag="VFU15_12690"
     CDS             complement(18485..20284)
                     /locus_tag="VFU15_12690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718690.1"
                     /translation="MKKIIIPAVVLFAGCVVYFTATHSTTTKTNNSIPLSATVPVRNA
                     PGTRHPAEFRPRFSAVNVPAQMKQQYKVSASASQVITGNCGTMIFIPDHAFADKNGNA
                     VKGKVKLELVEGIADADIMKMNLGTMSDQGILQTAGMISISAFAENGDSLQLAAGRQL
                     DVEIPAAHHRNGMMLWDGKTNADGSITWTNPQQLNNGLREVPLETLADSSQSGAKKEK
                     RSDHIATKIHASWGFSYWSGNGSGIITGASLMRAYKVVNRPGANAADTVFRWKTGMPG
                     GDSVAAVLNRYERNGFFEDDEATFFSDSGNVVLASNGYDRNGNKTTLDLTDKKFLHTN
                     IATAEFRSRLPFIRQCCDAGVIDCYTSFPTRELWKSDLAAADLLKKSGCALESFFRAL
                     GNKKQDNIDPKDPKTAAALDAARTEAIRSYSKKVHALEAAEESYSFGMKKLGWANVDC
                     LYHNSTAGAAPFFLNAHVDGVNEDEDPQVTLMVPSQGIHIPGYRRPNGDYSFTHGEYE
                     QSAYYPKGEKGYILVRSGAGDKLRYALKEIVFGENAVESLTMKAGTEDELAKALGNAP
                     PQKKAEDESLVDDWYTRALNSGGGCLCDYGVEK"
     gene            20397..20957
                     /locus_tag="VFU15_12695"
     CDS             20397..20957
                     /locus_tag="VFU15_12695"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU4718691.1"
                     /translation="MSAYNSETELLAGLRKGESAAFRRVYVLHYNMVRHLVISNSGRE
                     EDAKDLFQEALVVLFGQLSGEAFTLSSSLKTWLYAVCRNKWLKQLEKNKRNVRFTDFE
                     PAENISVPAEKEVDEHHSLLRKSLEQLGVQCRKLLLLFYYFHKSMDEIAAELHYTNAD
                     NAKAQKYKCLQKLKSIYRQNPAADGH"
     gene            20947..21510
                     /locus_tag="VFU15_12700"
     CDS             20947..21510
                     /locus_tag="VFU15_12700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718692.1"
                     /translation="MAIDENTYELIDRYLQDLLSAEERTAFESRLEQDSEFREETGWM
                     KKAVGVVNDLGRDILKKQISAAISGVPSSALEKYRPSLNGKSFLQKWWWAITCGAVVL
                     IAAVLLFLHFRHPAIPEKQTGDRPLKQQDTVNVVPPIKDSVCDSMPERKTDNASSGSA
                     RDDEYEKRAKPGNRSSNRLRPRQEEMK"
     gene            complement(21564..23219)
                     /locus_tag="VFU15_12705"
     CDS             complement(21564..23219)
                     /locus_tag="VFU15_12705"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEU4718693.1"
                     /translation="MKKLIAVIILAMLAGDLFAYSKYWITFTDKNGTPFSTAAPSAFL
                     SSRSIQRRQNQGIPVTYDDLPVNPSYVAQVLATGAVTLNYTSRWMNAISITTTDTAAL
                     SAIAALPFVSGTAPVKRLMQNSGPSEKPNDISWTGTAGERTALPAYSPGSYSYGNSYG
                     QVHQINADCLHNMGYNGAGMIIAVLDDGFMNVNIIPAFDSIRLNNQVLGTYDFVAGTQ
                     NVYGVGGHGTMCLSDMAANLPGQMIGTAPGASYYLLRTEDANSEYLIEEDNWVAGAEY
                     ADSVGADIISTSLYYTQFDDPSQDHTYADMNGQNTIAARAATFTARVGMLTFACAGNS
                     GTSAWFYVGTPADADSILAVGAVDASGNLASFSSRGPSYDGRIKPDISAMGLNAYVVN
                     TSGNVTTASGTSFATPLSAGGAACLWQANPTYSAMQLRNAIRLSGDQASAPDNNKGYG
                     IPDYCQAGILLAQEDIHAPGPSFSIFPNPNNGRTTFSCSLDAGQTGTIEIFDAMGKCV
                     KTIQLLPGQQTAEEDLADLVAGIYFCRMTVNGNFEAEKKMIVE"
     gene            complement(23216..26635)
                     /locus_tag="VFU15_12710"
     CDS             complement(23216..26635)
                     /locus_tag="VFU15_12710"
                     /inference="COORDINATES: protein motif:HMM:NF027232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEU4718694.1"
                     /translation="MKKFSALLSMVILFAATAFAQDYLNQPNLHNRPADKKATFWDIQ
                     KAFETAWDGRTPSDTESENEEGGGWQQFKRWEWFAEQRTYPSGNFPDPGILFDQYRVY
                     KTQYANQNARSASASWSFIGPHVVPGNGGGAGRINCITFDPNNSNIIWIGSACGGLWK
                     STDGGATWSSNTDLLPSLSISDIVIDPTNTQTMYIATGDKYGIYWQYETWGHYSAGIL
                     KSTDGGNTWNPTTMNYSLANVVVIQRLIMDPSNTSVLFAATNAGIFKTTDAGVTWNNI
                     RSGKYYDIEFNPGNDNIVYAGDSTGFLRTTDAGVTWNYISGVTSGGRTSIAVTPANNN
                     AVYVWCENGGNVFTLYYSSNAGVSFSQKTDPSPNCTPYGYYDMVIEASPANANVLFAG
                     GLDIARSTDGGTTWNTVSDWAGWPNTNYCHADNHAQKFLPGSSNTIFSCNDGGIFKST
                     DQGNTWNDLSGGIDIKQYYRMSSSFLTPSLMYAGAQDNGTDRITGLSAATQVNGADGQ
                     TCLVDYTNDNVVFVSSQGGYFLKSTDGGNTFNALTSFGCDWTSPLVMDPVDNNIMYMG
                     SSDVLKSTDNGNTWNNISNGYFDGSCVYSLVVSPANTNYIYAATFGNIYRTTNGGNTW
                     TTITGTLPVGSAAITGISVSDNNPDALWVTFSGFSPGNKVFYSSDGGASWGNVSGTLP
                     NIPVNCTVYQNGSNDIVYIGTDLGVFYTDATLNDWQPYNTGLPNVIVDQLEINYPTSK
                     LRAATYGRGIWESDLQVSTLVNLDASVFAMNSPPSTTCDTSIVPVLRIRNAGMDTIFN
                     VELHYRMDNLAWQQYNWNGTLASLGTANIVLPLYTLSPGTHTLKAYTASPNSGADQNA
                     QNDTLVRTFTILSSSPPSTTAPPVSEGFVSSTFPPANWTMENSSGLWSRNTTYGGYGL
                     SSQSAMADFYYISSGTDMIVTQYVDFTNAVAPITLWFDVAYAQYDVAYNDSLIVDLYS
                     DCPGIGERVYARSGSALATAPMTQTVFAPLSNQWRTDTIHLDSLAGRGPMEIRFIAKS
                     GYGNELYLDNINLNGNALGIYSQSEQDGLDVYPNPANGTMNVVISAAQEGSTAISIYD
                     LTGNAVYVSSQRTASGENTIPVDVSGLAQGVYLLRVETDGKTTSRLISVSR"
     gene            26745..28145
                     /locus_tag="VFU15_12715"
     CDS             26745..28145
                     /locus_tag="VFU15_12715"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEU4718695.1"
                     /translation="MLRLLLILALLPGILLKAQVPFFETYFGSPRPDYARSLKQSADS
                     SIYIAGYSIDTIAGHSDVALSKLSPAGQLAWTKYYSDSLDNNCLFLQDAGDGSFFLCG
                     ETQTAANALDGFLMKVDTAGNVLWRKLYGGPQNESINRVVVLADGKLAMTGFQTDAGG
                     SNGIYVLVTDSAGNEMWSGNYGGQNNDAGRAVCALPDSGFVVFAETAVRSAVNIALEG
                     MRFDKSGIVLWDSIYDYGDTLPEGCQGMMLLSNGNLFVYGESQPTATTWFDFLFHELD
                     VNGNTIWSRKTGGPLPDAAFSMLETPGGFIGTGYSRSYSPGPNDIIVFGTDTVGTITW
                     AQPYGGPGIDIGYEIIPAAAGGYYITATGNVNGNDQFGLLHIDSAGSTGIAGSQQDGD
                     VRFFPNPASDQLTITTGAPGNYTVQVFSAGGQEISCTQKNGPLFGLDVSGLPAGMFFV
                     KISGENFSRTEKLVIR"
     gene            complement(28146..28379)
                     /locus_tag="VFU15_12720"
     CDS             complement(28146..28379)
                     /locus_tag="VFU15_12720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718696.1"
                     /translation="MTRNQFLTINDQPCRIKLKSGKEVFGVIWEKAHPGGCAYYFSSL
                     GERFSRASSVGMIIDLEDIVGAELLRDSGTLVG"
     gene            complement(28518..29039)
                     /locus_tag="VFU15_12725"
     CDS             complement(28518..29039)
                     /locus_tag="VFU15_12725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013305553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HEU4718697.1"
                     /translation="MLSAKIEKALNEQVTLEGNSSQYYLAMASWAETQGMNGVAQFLF
                     HHSDEERMHMLKLIRFINERGGHGVVPALAAPPKKFKSVHEAFEEVLKHEVKVSAEIN
                     KLVANCLKERDYTTHNFLQWYVSEQIEEEALARRVLDKLKLIGGEKGGLYLFDRDLEG
                     LAAAEASAETKGA"
     gene            29261..29857
                     /locus_tag="VFU15_12730"
     CDS             29261..29857
                     /locus_tag="VFU15_12730"
                     /inference="COORDINATES: protein motif:HMM:NF013814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HEU4718698.1"
                     /translation="MKKTILRNTLAIASAAFLFASCASDHGLTIEKRHYRNGYYVSTG
                     KSHDVASSSSVRIAPAATAPVNNETVLSSGNAQTASVTDQKTTPTIAQKKTPAAASRP
                     GRSSVILPAIFPHAVHKAQKMNSAKKPASAFPSSEKTGHTDDNTLLLIILCFLLPWLA
                     VLLKEGGPTTYFWISLLLWLCFWIPGVIFALLVVLDVI"
     gene            complement(29867..31246)
                     /locus_tag="VFU15_12735"
     CDS             complement(29867..31246)
                     /locus_tag="VFU15_12735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013634303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein B"
                     /protein_id="HEU4718699.1"
                     /translation="MDRAAEFILSAEKNVFDTELHRKLDFNIANYNQKVALGKQQFAN
                     LELARTRASVMRNRAIENLDKYLIEFEANFIRRGGKIIWAQDAAEANREILQVLKKSG
                     TELVVKSKSMVTEEIGLNEFLKKEGIEAVETDLGEYIQQLAGEAPYHIVTPAMHKSKE
                     DVAKLFHEKKGTPPDLSPAQITEWVRDHLKEKFRKAGAGITGANFIVADPGAIAVTEN
                     EGNGLMAMAFPKTHIVVTSIDKIIPALSDLDLLWPLLATYGTGQQVSVYNSIITGPRQ
                     PGEHDGPEELVLVLLDNGRSNLLAQVEQRRALQCIRCGACLNACPVYTAVGGHTYNAV
                     YSGPVGAVVMPWMAGMQEYRHLSYASTGCGKCSEACPVKIDLHKLLAHNRRDAVTAGP
                     AAKTETVMWFFWKGAMMKRSKMDKGGAKLKNFMLRQFFRKSWGERRELPPVAQKSFNQ
                     VWRERKGIK"
     gene            complement(31259..32827)
                     /locus_tag="VFU15_12740"
     CDS             complement(31259..32827)
                     /locus_tag="VFU15_12740"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEU4718700.1"
                     /translation="MSVKKISLFFPIAFLLLAFTGGKDPVREKYAALITAEDMKTHLS
                     VIASDEYEGRETAMPGQKKAAEYIAKQFASFGIPPYANGSYFQDVPLIEYRPSSGSVT
                     TPKNKYEFGKDFFFPPSDASRHEKCDTLVFLGYGIDDPAYNDYANTDVSGKAVLIMDG
                     EPKDRNGNNLISGSKAPSEWYVKRAKKVSEAQKHNAGALLIVVDEYDKALAKIMSHDH
                     MSMLVPDEENDVSVIYDEMPVLLITRSMADNLLKEGKQTPLEKLVEKISGKKKPVHTF
                     SKMKMDIVAQSTGRSVHSENVLGYLEGSDLKGQLVVITAHYDHLGKDSVVYNGADDDG
                     SGTVSIIELAKAFAQAKKEGHGPRRSILFMTMTGEEKGLLGSSWYTSHPVFPLDSTVC
                     DLNIDMIGRIDTTHAADTNYVYVIGSGMISAELKTINETENNTYTGLSLDYRYDKKDD
                     PNRFYYRSDHYNFAKNGIPVAFFFNGVHPDYHRETDEVQKIHFPLMEKRAQLVFYTAW
                     EIANRNERLKITQK"
     gene            complement(32906..33742)
                     /locus_tag="VFU15_12745"
     CDS             complement(32906..33742)
                     /locus_tag="VFU15_12745"
                     /inference="COORDINATES: protein motif:HMM:NF013033.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEU4718701.1"
                     /translation="MANNFATTGYQPGQVVSDFRLYDTAGVAQQLTTILAQNKPLLLI
                     TVSLTCPLSRHSMTYVLPDIYIAYHNSVNFLLVYVVEPHPDTPDVSPYSGSVWITQSN
                     LQDSVLLRQPRRYGTRRHFARQFLIRMHPPASIPLVIDDTANSYWNTFGPAPNNAYLI
                     TPQGMVYRKYGWFNGNKDSIMNDIPRLLATVGIDEYAQADAVSVFPNPSLSGFHVSVP
                     SENSFEYTVFDGEGRVIEKRNVDGNVVEFEEDHFAAGIYTIIITGQSGKMYRQRIITG
                     IR"
     gene            complement(33943..34386)
                     /gene="rpiB"
                     /locus_tag="VFU15_12750"
     CDS             complement(33943..34386)
                     /gene="rpiB"
                     /locus_tag="VFU15_12750"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013634329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HEU4718702.1"
                     /translation="MTKVRLAIGCDHAGYKLKEVIIRHLSANGISTRNFGTDSGESVD
                     YPDFAHPVANAVENGEADFGILICGSGNGISMAANKHAGIRAAVCWKPEIAGLARMHN
                     DANILSLPARFISDEDALRCVDIFLSTPFEGGRHANRVNKIPCHN"
     gene            34890..>35325
                     /gene="nadA"
                     /locus_tag="VFU15_12755"
     CDS             34890..>35325
                     /gene="nadA"
                     /locus_tag="VFU15_12755"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002998506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HEU4718703.1"
                     /translation="MNTIEYDVDEKVSRLDPGLDLFYEIGKLKKEKNAVLLAHYYQEN
                     DIQDVADFIGDSLGLAQQAAKTKADIIVFAGVHFMAETAKILNPHKKVILPDLRAGCS
                     LADTCPADRFSEFRKRFPDHVVISYINCSAEVKALSDIICTSS"
BASE COUNT         8037 a         9267 c         9074 g         8947 t
ORIGIN      
        1 ctgggcgatg gtctgcatat tcatcgtaac caacagaaca gaagatccca gatttgctgc
       61 ggcagcggca gcttcgcacc cggcgtgtcc tgcacccaca acaataacat catattcccg
      121 cagaagactc atgccgcgaa gttacatcat tcgtacgttt cacgtgaaac aagaagcccc
      181 gcacaggcgg ggctcgaaac tatttctttg aaaaattact tctgcttatt cgtcccgtcg
      241 gcattcaccg gtacatagta gatattgttg tgaacatcgt cgctatcctt gtaatcgact
      301 tgcagctgct gctcggtcat actcacaatc ttaaatttcc ttaccacgcc agcatcgctg
      361 gtgagcgtaa gccagattcc tcctttatcg aggacccatg tgccaccttc ggcaacgcca
      421 ttgtagataa gacgatatcg gccgttttcc tgtactacca gctgatcatt cttttgatca
      481 tcttgcgggc ctttttcaac tccgaatacc tccgttttgg acaaaaccca tgtccggcaa
      541 agaatttttt ccttaggcgt aagagtcgac tgcgttgcag aggcacctgt ggtcccgctt
      601 gtggcggttg tcggctttga cgtttgggca aaagacatca agcctaatga aatgaatccg
      661 atggtaaaaa gctttttcat gtttccgggt ttagaaggta cgtaaagcta acgctttttc
      721 ttcctgcctc cgcatgcgtt ctttttcagc ggaggtttta tcgcgaaagc cgacaaggtg
      781 cagcaatcca tgtgccataa cgcggtgaag ctcgtcacga aaagtgcaac cgtatttttt
      841 tgcattctcc ctgacgcggg ctacactgat gtatatttcg gcggaaatct ttttttcccg
      901 gcccacacga agatcaaagg ttataatatc tgtcagcgta tcgtgcccga ggtattccgt
      961 gtttatccgc agcagcttcc ggtcggtgac aaaaacataa ttcagggaaa ggatatcata
     1021 cctgtgattt tgagctatac cgatcagcca ggctcgcact tttcccttgt ccctgagtgt
     1081 aagttttacg tcctccggga agaaataaat ttctgaagaa ggggaattca ttaattgggg
     1141 ctgatgacaa attccaggga aacggatttg ccattgtcgg aaaactcgac cttatcggcc
     1201 aggtggcgca tgagaaaaac gccacgcccg ttcggctttt ccaggttctc cgggttcgta
     1261 ggatcgggga ggttgttgta atcgaaaccc gggccctggt cggaaatgct gaacataagg
     1321 gcattgtccg tagcggaaac attaagattt acgttcttct gggggtcata gcggttgcca
     1381 tgctgcatag cattgttcac cgcttctgtt accgccacaa gaatgtttcc gtaataatcc
     1441 tcggacacat tacaggccgt acagatctcg ttgatcagct tctcgataat tccaatattt
     1501 ccgggaactg atgagataga tattttttgg tcccgaatca tctcttcggt atgggtcatt
     1561 tctgctgaac tccgttaaaa tagttgttta ccttgttctt atagaaagga gaaagggccg
     1621 ggggaacagt ccgcaataat tccgcttctt tctgtttctg cctattatac tctgaaaacc
     1681 cgttagggtt actctgttgc tgattttttg cttcgtttgc ctggcgctgg ttatccatat
     1741 cgcgttgctg ctcggccttt tcgaattgca gaagcttatc cagaatatcc tgctggcgct
     1801 gcagggtttg tgcattgata ttcttgttga ccaggtccgt ttctgtttgc tccattttct
     1861 gctgcacctg tccgcccgga tcaacgcctc cgccattggg atctttattg tcctgctggg
     1921 cctgctggag catcatgcga atatactcct gctgggcggc gagttttgcc agctgttctg
     1981 cgttttgccc gttgggatcc tgggaaagct gctgcatctg ctggttaaga ctttgctgca
     2041 tctgccggag gctggacatc ccgggcttgt tctttttccc attcccgggt ttgttgcact
     2101 ttcccccgga acactggctt ttgttctgct gattggcctg ctgcatcata ttctcgagcg
     2161 actcattcag catgagggcg agattgttga tcgacgtcat ggcgatctgc tggcggtagc
     2221 gcgcttcgcc ggtattacgg tcgccgagcg ccaccattgc gcggtccatg ttctggttga
     2281 tattcgtaac ctcccggttc acgagcggag aaatctgcgg attgcgcttg ctcaacgcca
     2341 gcaggctgtc ttcgatcatc tttgaatcat cgctgagctt tttctgctcg cgggcgagag
     2401 ccgggtactg cggatcattg acatccgtat tctgcacctt tgccatcaac gcttcctggt
     2461 caaacgaaag ctgaataaga ttgctgagca gctgacgtat ggcctgcata tcttccgcat
     2521 tttcctcttc gtccattttc ttttgcgctg cctgaagctg ggccgaaaga tcctgcattt
     2581 gctgcgctgc gttcttctgg ttctgtgatc cttttttaga atcaccttca ttcaggttct
     2641 ggctgccttc ccgctgctgc tgctggattt ccatttcctg cagatcggta ttgggaatat
     2701 cgtgcggact gtcgagctta ctgttctcgt cctggatctg gtcaagattc tggcggagct
     2761 gctggaattt gctgttaagg ctgtcctgct gcttttttat gtcttctttc tgctgcttcg
     2821 aaagattttt ctgtgcagaa agatttccca gcgaatcctg ctgcttctgc atctggttaa
     2881 ggtcctgtat tgcctggtcg agcttctgct gcacgagcat atcccggaaa aggttgagcg
     2941 tgcggtcgag atccttctgg gcatcggtgt tttcctgctt catgctgtcg agcgccgcct
     3001 gcgttttgtt cttatctgca ttctgcagca attgctgcag ctgctggatc atccgcttca
     3061 tgtcgtccgt tttcagctga tcgaagaggt tctgtatttc ggactgtttc tgctgaaggt
     3121 cgggatcatt gggagaaaat tccgactgct gctgcatgtt ctgcgcattc tgctgcttga
     3181 ggttatcgat tttcgtttgc agcgcctgct ggcggttctg aagatcctcc agctttttct
     3241 tgtcttccca cgtgagcgat ttctgctcca tggttttttt gtagaggtcg tcaatatctt
     3301 tctgcagtgc tttggattcc gcaagacttt cttcgagctc cgtttcggtg ttcttgttgt
     3361 tggcgtcgtt ctgctggttg agctgatctt tcgtaggagc gtgatagatc attttctgcg
     3421 accgcgtgga ctttgcgcca tgcactccgt cgttgtccca cacttcgaaa taatattcca
     3481 cctggtcgcc catgccgata ttcagccggg aaagatccca gtaataataa aactggtcgc
     3541 ggacgagctt atccgaaacc ggcaggttaa caatctgcgt tttatccggc agctcttttc
     3601 ctgtcgagtc tttgtgactc aggaaatgat aaacgaacgc gagcttcgtg aacccgtaat
     3661 catccttcac ctcgccggta aaatacatgc gaagacggga aaccgagtcg ttcttttcat
     3721 tgacggaaat ggacgggtaa agatccggga tgacgttcac gctgtagcgg atggagtcgc
     3781 ggcttttgag aaattcattg gccgtgtgca gcgaataatt ccggctttcg agaaaacgct
     3841 gcgtgaatgc gaactggttt tctcccgtcg gcttgagcga ataggacgta tcgctgaatg
     3901 cgaaaagaaa attctttgtt gcgcgtgtgt tgaaccgcca ggtgacagac gttcctgcag
     3961 gaatgacaag atcgccggtg ttcttcagcg tttcgtcttt tttgccaatg tatttcgggt
     4021 aatgcaggtc gacatcgaaa ttcagcacaa tgggattggg caacacctcg agcgtatatt
     4081 ctttcgaggt aaaaccgtca gcggaaaggc ggaacgtctg ggttttctgc aggttgcgaa
     4141 acacgtagct gaagttgatc gtgttgtcgc ggttcatgcg gtattcgttc ccgtcgacaa
     4201 gcatgtagac tgtttccggg atctcagagc cggtcagttt cacgtgaagc ggaaaatctt
     4261 cattctccac gcctttcagg tcctgcgcga tctcgaagcg gaacggagcc tgcgtttcat
     4321 aatcctcgcc gtgattcaca aggcgccgcg ttccttcgga aagaatcccg gcgttggtga
     4381 aaacgataac cagcattacc agcagaggga tgacggcata gcgtgcgtat ttcctgtttt
     4441 cgcgcaggtc aatggcggag gtaaacggaa cggggcgcag ctcggaaatt ttctggctga
     4501 tgctggcttc gatgagcgca cggcttgcac cggaatccgc agcggacgat tttttcagct
     4561 gcagcacatt gagcagcttg tcctgcacat tggtaaaatg tttcccgatg atctcagcag
     4621 cctgctcgtg cgaaatgatc ttaccaatac ggttgagctt gaacaggggg agcgcgacaa
     4681 aacgtcccag cacaaaaagc gtggcgccga tgaaggaata aaagagaact gtcctgactg
     4741 acgtatcaaa atgcccgaaa tattcggaga cagtaaaaac aagatacccg gccagcagaa
     4801 ttcccactgc atagagcatg ccgcggatga gcatgttctt gtagtatttc ctgataaact
     4861 catcgagctt gccaaggagg agttcgtagc tgccggtcgt attttccatt agttcattcc
     4921 ctaacaaatt tgcgtaataa taacggcatt tccgagtaaa cgttacggtt acgggggcaa
     4981 tagtttgtta aaagatgcgt ttgataaaag gaatccacat cccggcacat ttacccttat
     5041 acacgatgaa aaagggaacg ttcatttttc accgcatttt gcgaccggtg ttgatcgcag
     5101 caatcgcatt catgacgacg atcgcggtgg aaagttgtca ccaggcatgc aagcatgcgc
     5161 agaaaacaaa tgcggcgctg gcaacaggca cttaccgttt cgggatgaaa aaacacagta
     5221 agtacaaacg gatgaaccgg aagagcgcag ggtaaaagac cgctttcagg cggactgtcc
     5281 attgagattt atccgcgggg aagaatgaaa ttatacgtga tggcctattc ttccgaaaac
     5341 gcccagcggc aaaacgctgc cgtgtttcgc cctgaggaac aattccccgg tttccacttt
     5401 ccgtcccgaa aagagatttt ccaggatcat tgccgaaaac cccagcgaat acgcattgag
     5461 gatgagaaag tgatcctttt catcgagcag ctcaacgacc gccttgacca tttcgttgat
     5521 attctcttcg agcttccatt tttcgccgtt cggtccgtga ccgtatgccg gcgggtcgag
     5581 aataatgccg ttgtacctgt tgccgcggcg ctgcccgcgt ttcacgaatt tgagcgcatc
     5641 ttccacgagc cagcgaatat tgtccagccc ggaaagcgac atgttttcat tcgcccacgt
     5701 caccacctgc tttacggaat caacatgcgt gacatccgcg ccggcagcac gcgcggcaag
     5761 ggacgctccg cccgtgtagg caaaaagatt gaggaattta ggcgcctggt ccggcttctg
     5821 cgcagcagac attttgcgga cggatgaata aatataatcc cagttcaccg cctgctcggg
     5881 aaaaacgccg acgtgcttga acgccgtgag cgcaaggcgc agcgagatct ttttcccgtc
     5941 ctcaaattcg tacgaaatat tccagcggtc gggcatgtcg cgtttcagct ttttccattc
     6001 accggaagag ctgcttttcg gaacgaattt cacatccgcg atcttttccc attctttctc
     6061 attctgtgcc ggatcccaga cagcctgcgg ctcggggcgg atcgtgatgt attttccgaa
     6121 acgttcgagc ttgaggaaat ttcctgagtc gatcagctca taatctttcc agcgggaagg
     6181 ggagaggaga agcatgcgca aagatagccg agagaacgga gtatggagaa cagggagcgg
     6241 cggtttgtca gaactccgtt ctatctttgc ggcgcaatgg aaaacagaag aacacgcgtg
     6301 cgttttgcgc cgagcccaac aggtccgcta cacatgggcg gcgtgcgtac ggcgctttac
     6361 aattacctga tcgcaaaaaa agccggcggc gatttcatac tccgcattga agacaccgac
     6421 caggaacgtt tcgttcccgg agcggaagaa tacatcatcg aagcgctgaa gtggtgcggg
     6481 atcgaaccga atgaaggaat cggtttcggc gacgggccgt ttgcaccgta tcgccagagc
     6541 gagcggaagg aaaaaggaat ttacaatcaa tacgtgcagc agcttattga ttcgggcaac
     6601 gcctattacg cgttcgatac gaaagaagag ctcgatgcgc aacgggagcg tttcgaaaaa
     6661 atgtcgaaaa cgttttccta caatgccgta acacgccagg aactgaaaaa ttcgctttcg
     6721 ctttcggaag acgaagtgaa aaaacgcatt tcgtcgggcg aacattacgt ggtgcggatg
     6781 aagatcccgc gcaacgagga agtgcgtttc catgacctcg tccgcggatg ggtagtggtg
     6841 cacagctcgc agctggacga taaagtgctt ttcaaaagcg atggcatgcc tacgtatcat
     6901 cttgcgaacg tggtggacga ttacctgatg cagatcacgc aggtgatccg cggggaagag
     6961 tggcttcctt cggcgccgct gcatgttttg ctgtaccgtg cgttcggctg ggaaaacgtc
     7021 atgccggaat ttgcgcacct gccgctgctg ctgcgtcccg acgggaacgg aaagctgagc
     7081 aagcgcgacg gtgatcgcct cgggttcccg gtgttcccgc tttccggtga actcctggac
     7141 gtgaaaacaa aaaagccgga gaagttttcc ggttaccgtg aagccggtta tttccccgaa
     7201 gcgttcatca acatgctggc gctgctgggc tggcacggaa gcggcaacca ggaattgtat
     7261 tcgaaagacg agctgatcca tgaattttcg ctggagcgcg tgagcaaatc gggcgcgaag
     7321 ttcgatgcgg aaaaaacgaa atggttcaac cagcagtatt tgagaaagca ttcggatgcc
     7381 gggcttgcgc agctacttat tgccggcggt caattgcctg ccgctgatcc cggttttata
     7441 acggaagtgt gccggctgat gaaagagaaa gtgaatttcg tgcaggagat ctatgagcag
     7501 ggaaaattct ttttcgatga tccttccgca tacgacgatg gtgtgatcaa aaagcgctgg
     7561 aacgagggcg cgaaaaattt cataaccgcg cttatcaatt cgttttccgc ggtgaattca
     7621 tggacggcgg ccgattgcga agccctgttc aagaagacgg cggaagagac cgggaccaac
     7681 ccgggtcacg taatgcagct gttccgcgtg ctggtgagcg gagcaggagg aggaccggtg
     7741 cttttcgaga tgaccgcgct gctcgggaaa gaaactatcg ttcgccggct gaaaaccgct
     7801 cttgaaaaac tggcctgatc atccatgggc atcatcaacg taaacggtat ccgcgtttat
     7861 gcgtaccacg gctgtctccc ggaagaaacg aagatcggcg gggaatacgt tgtggacgtt
     7921 tccatcgaaa ccgatttttc caaagcggcg gaatcagatg atctttcgga gaccgtggat
     7981 tattgcaccg tgaatgagat cgtgagaacg gaaatgacgt tgccttcgaa actgatcgag
     8041 cacgtggcga aacggatcgc ggatcatctc cgtaaaaaat ttcccgcagt gaaattgttc
     8101 cgtgtcaccg tgacgaagat caatccgccg atgaacgggg ccgtggattc tgtttcggtt
     8161 gtcgtggagg gatgattttt tcccggcgga aaaacgttac ttcgctctta ttgaatccgc
     8221 ataaggccgc agatcgtaaa tgcgcgcctg ccggaaccgg cccatttcga attttgtata
     8281 gggaagaata tccggatcgt actgcattcc ttcgcaatta agaacaagat aattcgcgcc
     8341 gtatgaaata aaagcgtgaa tgcggtcgcc cctgttgccg agcgaggaat agctgcagtc
     8401 ggtatatccc ttgcggtcca tcatcacaag actgtagtcc gggctgggat ccgggatgct
     8461 gatcacaata tcatcgcggg tgatcttcag ggaatcaaac acgggcctgg cctcctgcag
     8521 gttgatcatg tccttttcgt atgtgtgatc gaagttgtgc catttgctgt attcttcctg
     8581 cgaaataaag acggacgttt ctgcaaaagg atcgatggac gaatacctga tgcgggtgat
     8641 catcgcgcaa ttccatgcgg aaaaaagaag aacaaccccg gagaccacct gcacgcggcg
     8701 gtcatgaaaa agagacgcgg cagttttgcg cacgtagcgc gcggcaacca gcaggagaac
     8761 cgcgacgaac gggagcatgt tgacgaggta gtaatcgtga accgtgaaca cctggaagaa
     8821 aaggagcagg tacagaagaa cgatcagggc cgaaaccgaa aggataaata cagaaacctt
     8881 ccccatgcgc cgccagtcga ggaacatcca caacattaca ccggacagaa aaaacaggac
     8941 cggccggtta aaatacacgg gaagaagatc attcacgagc gatctcgatg tcaggacgat
     9001 ttccgcccat gtcatgtccc aaaccgggag tatgccgacg agaaaaactc cttcgtcatg
     9061 tgtcttgttg tagtggtctg cataaacata ccaggcagca accagcagga gaaccacgcc
     9121 ggcggaaatc attacagctg ccgggcgccg gaaaacagcc atgcgttttt ttccgggaag
     9181 cagtccggcg agcgcgagcg aacaaacgat gcccagcgta tacaaagaag tgatcttcat
     9241 ccagccggcg atggtggtga gtaatgcggc gaacacgaga tccgtccacc ggttccaccg
     9301 gtgatagcgg aaccagaacc acccgccgca aatggcgata cacattgccg acgcatccgc
     9361 aataaatgaa atgccatacg tggaaagaag cggcgtagta aaaaaaagca gggaaagccc
     9421 gagcgcccac cacgtattcc gcagggaaag ctcgaagatg cggaacaggc agagcagccc
     9481 taaaaacagg atgatgttct ggacggcccg gtatacccat ggatgataac cgaaaatcct
     9541 ccacagctga gcaacggaat aattcagcag gggcatttcg ctgactgttt cctgcggacc
     9601 cgccggagag cagaaatgca cctgcggatc gaggaacgaa cgatgtccga aataataatt
     9661 cgtggccatg gaatagcaat ccgcctggcg ccatgtatga tgccctctcg ggccgtacct
     9721 gtagatatag ggataattgt acagcaggca caggaccaca aatgcagcaa ggaaaagccc
     9781 aaacgatcgt ttcgggaaat aacgcttcct gaacatgaag taaacctact aaagaatccc
     9841 gatgtgaatc cgccggtttt acgtgcaaaa gagacagcgg cgcggtccgg cattccgcgt
     9901 tgccgttttt ttggcgaatt tgcggttcca aattcctgcc catgaaatat ttctttcccg
     9961 taatttttct ttttctcatt tcctgcggag gaaaaggaaa tcttcagttg ccgggagttt
    10021 ccattgaaaa aaacgcaaag ctggaagcca tgcagaaatt ccatatcgcc gcgctgaaag
    10081 cacaggacaa tatcctgctg tgggtggcca ttcccggatc agatgaaagc aaagtgctgc
    10141 tgtccgcttt catttccgga ggacatacat cgcctgaaac ggcgctgcgc tcctcgttca
    10201 ggaacgacac ggtacaccgg gcaaagatcg atacgctgat ctcgcattat gggacattgc
    10261 tcaacgcata caaagacgtg accagttccc ttaattccat tgctgattat aacgatccgg
    10321 cgagaatgct cccggcgcag gcaatggtgt atgacaacga cggcgcggtc cggatgctct
    10381 tccgccggtt cgaaaacgaa ttgaccgcgc tggagcagga atccgaagaa ccggcagccg
    10441 ttgtgatacg gtgaggcatc cccgccacat tgcgtttcca tcaaaacagg tttttcgtac
    10501 atttgtcgtc cctcaaaaag gtcctgtggc cgagtggcta ggctcagctc tgcaaaagct
    10561 gctacagcgg ttcgaatccg ctcgggacct caacaaatgc aatcgcccgg acaacatcgt
    10621 ccgggttttg tttttttccg gcgaccgttc ccgggaaaac aacaaaagcg tttcccgcaa
    10681 aacaggaaac gctttcattc atatccgcgg acatcagttt tgtttcgcaa gaaattccgc
    10741 caaagccgtt ccaacgaggt acgtccagcc ccctgcgaaa ttctccggat cgaaatcggg
    10801 attatccgcg gggaacgaga gtagcccgga atgcatcagg cgcagcctgg tttttccttc
    10861 ctccgggaac agctcgaatg tcaccagcga atttccggaa tagccgtcgt acctccagga
    10921 atacgtgagt ttttccatcg caacaacttc tgtcacttta cagatgtgga gatacgggcg
    10981 gccgggatct tttccgccgt aaaaccggaa ctcacatcct ttttcggccc ggaattccgg
    11041 aaggtcgaaa taccattttt tcatttcgtc tttgtccgta atggctttcc acaccctggc
    11101 gacggaagcg ttgaagatgc gttcaatggt gaaagcacct ggctgcatga ccgtgtacat
    11161 tttggaaaga tattccccga gacggttgag cgtttgcttt ccgccttcga tcgcgccgtg
    11221 ttctttttcg aggcgatcca attcttcttc cgacgggaaa atgccgcgca tcgtaagaaa
    11281 tgttttatca ccccgggctt caaacgtgat ctccgaacgg aagttcaccg gttccgtatc
    11341 gccgtcgtcg gaatgctcat acacgatgcg ctccggcttc accacctcga cgtaaacgat
    11401 cttgttcggg tagtttcttc cgtcgggacc gtggaacata aagcgccatt cgccgccggg
    11461 ctttacctcc atgttcttat gcgtcaggga aaatccgttc gggccccacc agtttttgat
    11521 gtgttcggga tcggtccaca cttcccaaac gagttcgcgc ggcgcgttga gcaggcgcgt
    11581 gtgaatgagc tcgcgtcccc tgcgttcgaa ggttgcggtt tttgtttcct gttccatttc
    11641 aatgcgtggt ttttgtttgt gcaacaaaat attcgtcgag gtttccgtgt gccatggtga
    11701 atccctgttc gaagcccatt tcaataatag tggcaaggtc tttttccgag gcaaacgtga
    11761 tctcaaccgt cacttttgtc cccgaagcgg ttttcccgaa ttccacgtcc cagtccattc
    11821 cgggcggctg gggaaaaaca gttccgttct cgtcgcagaa ggaatctttc ccgtgaaaaa
    11881 atttatgggg aatgatcttc atgtaatcgg cccttgccca gtggcgttcg ccgttcgggc
    11941 ccaccataca gtagagccag cgaccgcctt cccggaaatc catttccttc gtttcggcct
    12001 tccagggttt gggcgcccac catttgtcga gaatgtcttt gtctgtccac gccgtccagg
    12061 tcatttcgag cggcgcggcg aattcccgtg ttacaaaaat ttttttgccg ggaaggtctt
    12121 tggtgaaaat cgttttcatg agcatttgtt tttagcggtg ttatttttta attgtttttg
    12181 accccgaaga ataaatactg ctgtgtgtac ttattgtgcg tgaacggcgc ccgttttttt
    12241 tctccagaaa atatacgaga cgacgccggg cgcaatgaac aatgccatag gcagcaccat
    12301 gagcggaggc gttcccactg cgagcacgga ataaaccgcg ccgaggagat cgaacgcgag
    12361 ccccgcatac gcccattctt tcacacgcgg aaaaccggga acaagcaaag ctgcgacgcc
    12421 gagcaccttc atcaacccga tgaaaggcgt gaaatacgac gggtacccga gcttcgacat
    12481 gaagcttaca gcatcggccg tattaaggac atccggcacg gccgagaaaa gcatcacggc
    12541 agcgaacagt cctgtggaaa tccagtaaat gatgttggtc tttttcattt ttcagcggtt
    12601 atgtgattta ttattctgcg ttaacggtct tttcgccgga gcggtcactt tctttttttc
    12661 ctgccgtttt ttttcttttt ggattgcagt tcttcgagat acgcttcgag cgcatccagc
    12721 ttcttttccc agaattcgcg gtactggttc acccagtcgg atacttcgtt cagcttcgac
    12781 agcttcgctt cgcaatagcg ttcgcgcccc tgctgcttca cgaccacgag tccgcactcg
    12841 gtaagaattt tcaggtgttt gtaaaccgcc gtgcggctca cgtcaaaatt cccggcgacg
    12901 gaattcacat tcagcggctg gtgcgcgatc atgtggagaa tttccctccg cgtcggatcg
    12961 gcaatcgcct ggaatacgtc tcttctcata atgcaacctt ttggttgcaa atatatatga
    13021 aaccttttgg tttcacaaaa tttttttcac ggccgcagtt caatttcccg gtgaatggat
    13081 ttccgctgta cgacaagcgg ctgcttttcg atatcctcct gatgttcgtc gcgctgatgt
    13141 atgcacgtct ttattttgac cggttcgcgg gaagacggaa aggaaaaagt tcttgcgcac
    13201 cgcatggtgg acgaggcccc ggttccggga taaatcgcgc cgttttaacc tctaatcatt
    13261 ctctaatgtt tgtgatgcca ttgcgtttta atttgtattt ttccattggc acacaacaca
    13321 ttcatggaag agccctgcag agatttccgc cgcgttttca cggctggtca gcgtttccgg
    13381 tatggcattt accagctttc cgatcatttc tttggacgaa aaaagttttt caataagagc
    13441 cggcaaaaat ttttccgttc gctggccgca catcttgaaa aacagggaga aggaaagatc
    13501 atgccggtgg agcggagaaa agacctttcc ctggaagaat tcaaatcgca ttatgtgcgg
    13561 aaaggaatcc ctgtcgttct cgagggcgcg gcgaaagact gggcgtgcgt gcagaaatgg
    13621 tcgctggaat atttccggca gctgcacggg aaagatgaga ttgtgctcgt ggaccaggga
    13681 aaaaaagaat acccgtacga gctgaccacg cttgccgacg ttatcgacaa tatccgcggg
    13741 ggaggaggga agtattaccg cttttacccg ctgctgacaa ggcacccgga gcacatcaac
    13801 gatttcgatt ataaattcct acgcgaacgg cgaacgagcc cgacgtggtt cgatgccttc
    13861 caggttttta tcggcgggga aaattcgtat acctgtcttc acaatgccaa ccagtcgaat
    13921 ttattcgtgc aggtgtacgg tgaaaaaaaa tgggtgcttt attcgcatta ctattccccg
    13981 gtgatcgatc cggatcccgt gcggaatgta tatcgcaacg cgccatcgaa aagagaagac
    14041 ggtcccttta atcctttcga accggacttc tcacctccct acgagctgta ccgctacctc
    14101 gacgggtatt cggttcacct gaagccgggt gacatcctgt ggaacccgcc gttttactgg
    14161 cacgctgtga aaaacactgc tgattcgatc ggtgtcggtt atcgctggct ttcccctttt
    14221 tattcgttca gggtctcgcc gctgtacatg ctgctggatt gcttcgcgac gaatccgccg
    14281 atgtggaaag catacagtct ttaccgcaag gacctcaacg agcttcatct tgcagaagtg
    14341 aaacagctgg aagaggcgaa gaaaaagatc gcggagcacg aagcgaaaaa ccggcccgtg
    14401 acagggctca gcgcgtgatc attattttct gcccgccggt ggcaacgcca tccacgaaca
    14461 tcctgcagaa ataaattcca tcatccagct gcgaaagatc gagcgatgct gtcgtcgctg
    14521 cggactgaat catcgtttca ctcacacatt ttcctgtcat atcataaatg cggacgcaac
    14581 ctgtttttcc tgcgtcgagc gaataagaaa cacttgctgt tccgttattc gggttggggg
    14641 aaatgccgaa cgcgcttttt gatgtaagtt catttattcc cgttggcaca cattgcgaaa
    14701 ccaggccacc gctgttctgg agcgagaccg gcgttccgca gattccaccc gacccggtga
    14761 gcgtgggcgc attcacggcg acgatcgcct gtgaaaagac attcgttagc gggcttgttt
    14821 cattgcagcc ggagctgttg cccagcgaat cggttttgat catgtaaaaa tcggccccgc
    14881 ccattccgaa gctggtggta tagcccgcaa tgaggaaacc gccgtcgttc gtctgctgca
    14941 ccgactggca cgcgtcgttt ccgctgccgc catacgtctt cgaccattgc gcattcccgt
    15001 tgcggtccgt ttttacgaga taaacgtccg tgttcccggc gccgaagctt tttgtattcc
    15061 ccgcgatgat aaaaccgccg tcgctgcaaa ggtgaacggc ataggcttca tcggccgatg
    15121 cgccgccgta agttttcgac caggtcacat ttccgcccgc gtcggtgcga atgagcagcg
    15181 catcttctcc ttccgttcca tagccgtttg taaaaccacc gatgatatag ccgctgtcgg
    15241 aaggaacctg ctgcaccgac catccatgat catcgcctgg gctgccatag gttttcgacc
    15301 actggaggtt gcccgacaca tcgaatttca cgaggtaata atcccatcct cccgcgccga
    15361 agctgtttgt ttctccgccg atgatatagc caccgtcgat tgtctgctga atgaaatgtc
    15421 cgtgctcatc gcctgcgccg ccgtaggttt tcgtccataa aagattgccg gcagaatcat
    15481 ttttcatcag gtaaacatcg tcgccgccgg cgccgaagct tcttgtgtag ccggtgtgaa
    15541 tgtacccgcc atcgggcgtt tgcagcactg cgtgtccttc atcccagtcg cttccgccaa
    15601 tggtttttgt ccattgcagg tttcccgccg gatccgtttt gatcaggtaa acatctgtgc
    15661 tgcccgcacc gaaactgttt gatcttgctg taatgatgaa ccctccgtct gttgtttgct
    15721 gcacaaaaca atccacttcg tccccgttcg cgccgtatgt ttttgtccat tctatcgcac
    15781 cgagcgaatc tgttttcacg aggtaaatat cccatcccca gacgccgtaa ctggtggtcc
    15841 agccgacaat gacataaccg ccatctgtcg ttgtttcggc gtagatcgcc tcgtcctgtc
    15901 ccggacctcc gatggcttcg ttgattttcg tttgtgcact taaaatgtgc gccgtaaaga
    15961 ccacggcaag gagggtgagc agctttttca tgtgtgtggt gctggtaacg tacagtttat
    16021 tccacgtccg cgtgcaaccg gacgcccggc cataacaata atatccaggc aaaaggaggt
    16081 gttactccgg aggcattaac gccacacacc ggcgtttatt atccttcaat ggttttattc
    16141 tttatccgtc gagctttccc aggatccgtt ttctttcttc ctgcatgaac gtcgtcagct
    16201 cgcgcgtgaa atcgtccagc tctcccgtgc tgcttctcac gtgggtgatc actttctgca
    16261 gctcgctgct gttcaggatt ccgccgtcgc ctttttcgag aagctgtata agaccgaggc
    16321 aagtgcacag gggtttccgt acgcgatgtg aaaccatttc cagcatttcc tcgagcgcgc
    16381 tgacgtaatg aagaagttct ttttccgtct gccggcgggt ttgctcacgg cgctcaatac
    16441 gcacgcgaac aatttccagg agcgtttccg catcgaacgg tttggtgata taatcatccg
    16501 caccgaggct catcccggta cgacggcttt ctttatctgc attcgctgaa aggaaaataa
    16561 agggaatgcc tgccgtctcc atccggtttc cgaggacgcg gcgaacttca aatccatcca
    16621 tctccggcat ggaaatgtcg caaagaataa gatcgggctg gttttttgcc gcatacccga
    16681 gtcccgtttt tccgttgccg gcggcaatca cctcgtaatt tccaaccccg agtatctccg
    16741 ctatgttctc acgcagaacc gggttgtcct ctatgacaag gatctttttc atgtttcaga
    16801 ataaggttca ttctttccca ggtccttttc gtacacgaaa agaaacccgg atgcatttac
    16861 cattggggga caacagcaga aacgattcag gggttcgttt cttttttaac gtgtacaaag
    16921 atatgaacac aatacgccac ccgcgctgat atagctcagg agaaaaaaaa gaaattgtca
    16981 gctgcgggtt cagattctga atgcgagcac ggtaatgtcg tctgtctgcc cgttctcgtc
    17041 ctgccaggcc agcaactgct cttcaatgag ctgcttctgc acatgcatgg ggcgttggca
    17101 aacttccttc aacagggaaa gaacacgttt ctgtccgaaa cgtttattct ccgcgcttcc
    17161 aagctggtcg gtgaaaccat ccgaataaat ataaaggcga tcgcccttct gaagatttat
    17221 tgtcctggaa gaaatttcaa actcgtgcgc gcctttatcc ccgatcgtgc atttgtcttt
    17281 ttcaagtgcg ataacttctc cgttgcgcac aaccaccccg cgcatgcgcg cagaggcgta
    17341 cgtcattttc agcgttttct tatcgatgca gccgatcatc aggtcggccg attccagcaa
    17401 atgcttttta cggtcgctgt gcgtgatgcg gttcagctcg gtgttcattt tacgcagcgt
    17461 ttctgccggg ttcctgcagc ctgcatccgc cagccggttc aggatgctga gcgcaagaac
    17521 ggaaatgtac gatgccgaaa caccgtggcc cgtgctgtcg cccacggcga acgtcagctt
    17581 ctttttatcc tgccgcactt tgtaaaaatc cccgctgatc atccggcagg ccttgtaaat
    17641 aagaaatgaa tcttcctccg cctcgtcacg ttcatcgtta ttgttgagga tcgttccgtg
    17701 aatgtatttc gcgtaaccga tgctttccat cagctcccgg tccattcttt tctgcgcttc
    17761 gcgcaattct tcttccactc ttttctgttt tgtaatatca taccgcacgc tgatgtagcg
    17821 ttccggtttt ccgttctttc ccatcaccgg cgcgatcgtc gccatcaccc aataggcgga
    17881 accgtccttt gcacgatttt ttatttcacc ctgccatact ttaccactgc cgatcgttct
    17941 ccacatttcc ttaaaaacag atgaaggcat gtccgggtgg cggataatgc tgtgcggctt
    18001 tcccattacc tcttccatgc tgtatcccgc gacctggcag aatttttcat tcgcatgaat
    18061 aatgttccct ttcagatcgg agatggtgag cagcgccgac tggttgaata tctcgagatg
    18121 gctcaacagc tctgcgcgga tctcatcggg gcggtcggga atgcaatctg ttccgggaac
    18181 gaatttttcc tgtacaggtg cgcccgggaa aaacccggcg agcgcccgca tgatctgggg
    18241 ttgcctgaac tcggtgatgg ctgaaagaaa tgacatgatg ctggggtata aaaaataagt
    18301 gtccgttact gcgtgctttt cacaccgcat aggtacggcg gaccggaagg gcgttcaaaa
    18361 cacgattacc gtgaattgca acaggtgtaa gatttcaggt ctttttgtga aatgaaaaat
    18421 ccccggcagc ggaacattgc cgccaccggg gaacacaccg cccggaataa attttatgtg
    18481 gcgtttattt ttccaccccg taatcacaga ggcaaccgcc acctgaattc agcgcacgcg
    18541 tgtaccagtc gtcgaccagg ctttcgtctt cggctttttt ctgcggaggc gcattgccca
    18601 atgctttcgc gagctcatct tccgttccgg ctttcattgt taatgattca accgcatttt
    18661 ctccgaagac gatttccttc agcgcatagc gaagcttgtc gcctgcgccg gagcgcacga
    18721 ggatgtatcc cttttctcct ttgggataat aagccgactg ctcatattcc ccatgcgtga
    18781 aagaataatc tccgttcggc cgcctgtatc ccggaatgtg tattccctgt gacggaacca
    18841 tcagcgttac ctgcggatct tcatcttcgt tcactccgtc cacgtgcgca ttcagaaaaa
    18901 acggggccgc cccggccgtc gaattgtgat agagacaatc cacattggcc cagccgagtt
    18961 ttttcattcc gaaggaatac gattcttccg ccgcttccag cgcatgcacc tttttcgagt
    19021 agcttctgat cgcttcggta cgtgcggcgt caagcgctgc tgctgttttc ggatctttcg
    19081 gatcgatatt gtcctgcttt ttatttccga gagcacgaaa aaaactttcc agcgcgcacc
    19141 cggatttctt cagcagatcg gccgcggcaa ggtcgctttt ccagagctcg cgtgtgggaa
    19201 atgatgtgta acagtctata acgcctgcat cacagcactg gcggataaac ggaaggcgcg
    19261 agcggaattc ggccgttgca atattcgtgt gcaggaattt tttgtcggtg agatcgagtg
    19321 ttgttttgtt cccgttcctg tcataaccat tggacgccag gacgacattt cctgaatccg
    19381 aaaaaaaagt cgcttcatca tcttcaaaaa aaccgttcct ttcgtaacga ttaagcactg
    19441 cggcaacaga atctccgcca ggcatcccgg ttttccagcg gaaaacagtg tccgcagcgt
    19501 tcgcacccgg tcgattcacc actttgtacg cccgcatcag cgatgcaccg gtaattatcc
    19561 cgctcccgtt cccactccaa taagaaaagc cccaggatgc gtgaattttg gtcgcgatat
    19621 gatcggacct cttttccttt tttgcgccgc tttgcgaaga atctgcgagc gtttccaggg
    19681 gaacttcccg caaaccattg ttcaactgct gcggattcgt ccacgtgatg ctgccatctg
    19741 catttgtttt tccatcccac agcatcattc cgttacgatg atgcgccgca ggaatttcca
    19801 catcgagctg cctgccggcg gccagctgaa gcgaatcgcc gttttccgca aatgcgctga
    19861 tgctgatcat tcccgcggtc tgcaggattc cctggtcgct catcgttccg aggttcattt
    19921 tcatgatgtc cgcatctgca attccctcga cgagctcgag cttcactttt cccttcacgg
    19981 catttccgtt tttatccgcg aaagcgtggt ccgggatgaa gatcatcgtg ccgcagtttc
    20041 ccgtgatcac ctgtgaagcg gaagcgctga ccttatactg ctgcttcatt tgtgcaggaa
    20101 cattcaccgc ggaaaaccgc ggacggaatt ccgccgggtg gcgcgtaccc ggagcattgc
    20161 gcacaggaac cgttgccgac agcgggatgc tgttgttcgt tttcgtggtg gtggaatgag
    20221 tggcggtaaa atacacgacg caccctgcaa agagcacgac tgccgggatt attatctttt
    20281 tcatggctgg attgttttcc tcttaatacc ggcaacccgc aaaaggtaac cgcggcggtg
    20341 aaaaaaattt ctgcttaaat gggttacctt tcggtttcac cgggataaac ggacagatga
    20401 gcgcatacaa cagcgaaacg gagctgctcg cgggcctgcg gaaaggggaa tccgccgcct
    20461 tccggcgcgt gtatgtgctg cattataaca tggtccgcca cctcgtcatc agcaatagcg
    20521 gcagggaaga agatgcgaaa gatcttttcc aggaggcgct cgtcgttttg ttcgggcagc
    20581 tgtccggcga agccttcacg ctgagctctt cgctcaaaac atggctgtac gccgtctgcc
    20641 gcaacaaatg gctgaaacag ctggagaaaa acaaacgcaa tgtccgcttc accgattttg
    20701 aaccggcgga aaacatttcg gttcctgcag aaaaagaggt cgacgaacac cattctttgc
    20761 ttcgcaaaag ccttgaacag cttggcgtgc aatgcaggaa gctgctgctg cttttttatt
    20821 acttccacaa aagcatggac gagatcgcgg ctgaattgca ttacacgaat gcggacaatg
    20881 cgaaagcgca gaaatacaaa tgcctgcaga agctgaaaag catttacaga caaaatcctg
    20941 ctgcagatgg ccattgatga aaacacatac gagctgatcg accgttacct gcaggacttg
    21001 ctgagtgcgg aagaacgcac agcgttcgaa tcacgcctgg aacaggatag tgaattccgg
    21061 gaggaaacag gttggatgaa aaaagcagtc ggcgtagtga acgatctcgg tcgcgacatc
    21121 ctgaaaaagc aaatttccgc agcgatctcc ggcgtacctt cctctgcatt ggaaaaatac
    21181 aggccctcgc ttaacgggaa atcgttttta cagaaatggt ggtgggcgat cacctgcggc
    21241 gctgtcgttc ttattgccgc tgttcttctc tttctgcatt tccgccatcc ggcgattccc
    21301 gaaaaacaaa caggagaccg gccgctgaag cagcaggaca ccgtgaatgt ggttccgcca
    21361 ataaaagatt ccgtttgtga ttcgatgccg gaaagaaaaa cggacaatgc ctcttccggc
    21421 tctgcacgag atgatgaata tgaaaaacgc gccaaacccg ggaacagatc atccaaccgg
    21481 ttgaggcccc gccaggaaga aatgaaataa gtggttcttt acaattgaat ccggcgctgc
    21541 cggtgacggt tgattgtccg cagtcactcc acgatcatct tcttctccgc ctcgaaattt
    21601 ccgttcacgg tcatgcggca gaaataaatt ccggcgacaa gatccgcaag atcttcttcc
    21661 gcggtttgct gcccgggcag aagctgtatc gttttcacac attttcccat cgcgtcgaaa
    21721 atttcgatcg tcccggtctg gcctgcgtca agcgaacagg aaaatgtcgt gcgcccgttg
    21781 ttcgggttcg gaaaaatact gaaggaaggt cccggcgcgt gaatgtcttc ctgcgcgaga
    21841 agaattcccg cctggcaata atcagggatg ccgtaacctt tgttattgtc cggcgcggaa
    21901 gcctggtctc cgctcaatcg tatcgcgttg cgcagctgca tggcagaata ggttggattg
    21961 gcctgccaga ggcacgccgc tccgccggcg gaaagcggcg tggcgaatga cgttccgctt
    22021 gccgtcgtca cgtttccgct tgtattcacg acgtatgcgt tcagtcccat cgcggaaata
    22081 tcgggcttga tccttccgtc gtaggaaggt ccgcgcgagc tgaacgaagc gagatttccg
    22141 cttgcgtcaa ctgcacccac agcgagaatg ctgtctgcgt ctgcgggcgt tccgacataa
    22201 aaccacgctg atgttcccga atttcccgcg cacgcaaacg tgagcatgcc cacacgcgcc
    22261 gtgaatgtcg ctgcgcgcgc agcgatcgtg ttctgcccgt tcatgtccgc gtaggtgtga
    22321 tcctgtgacg gatcatcgaa ctgcgtataa taaagcgacg tggaaataat atctgcgccc
    22381 acgctgtcgg catattccgc accggccacc cagttgtctt cttcgataag gtattcggaa
    22441 ttcgcatctt ctgtccgcag caaataatac gaagcgcccg gcgccgtgcc gatcatctgc
    22501 ccgggaaggt tcgcagccat gtcggaaagg cacatcgttc cgtgaccgcc gacgccgtat
    22561 acattctgcg ttcccgcaac gaaatcatac gtgccgagga cctggttgtt cagccgtatg
    22621 ctgtcgaatg ccggaataat attcacgttc atgaaaccgt cgtcgagcac ggcgatgatc
    22681 attcccgctc cgttgtagcc catattgtgc aggcagtcgg cgttgatctg gtgaacctgc
    22741 ccgtatgaat ttccgtagct gtaggatccc ggcgaatacg ccggaagcgc cgttctttcc
    22801 cctgcggttc ctgtccatga aatatcattc ggtttttccg atggcccgga attctgcata
    22861 agccgtttca ccggcgctgt gccggacaca aacggcagag cggcaatggc ggaaagtgcg
    22921 gccgtgtccg ttgtggtaat ggagatcgca ttcatccagc gggaggtgta attcagtgtc
    22981 acggctcccg tggcgagcac ctgcgcaaca taggaaggat tcaccgggag gtcgtcgtac
    23041 gtcacgggaa ttccctggtt ctgccggcgc tggatgctgc ggctggaaag gaatgcggac
    23101 ggagccgccg tggaaaaagg cgttccgttc ttatcggtga aagtgatcca gtatttgctg
    23161 tacgcgaaca aatcgccggc gagcatcgcc aaaataataa ccgcgattag ttttttcatc
    23221 gggatacgga aattaaccgt gacgtggttt ttccatccgt ttccacgcgc agcaggtaaa
    23281 cgccctgcgc cagcccggaa acatcaacag gaattgtgtt ttcacctgat gccgttctct
    23341 gcgaggaaac ataaacagcg ttgcccgtca ggtcgtaaat ggaaattgcc gtgcttcctt
    23401 cctgtgcggc ggagatcacc acgttcatcg tgccgtttgc gggattcgga taaacatcca
    23461 ggccgtcctg ttcgctttgc gaatagatcc ccagtgcatt tccgttgagg ttgatgttgt
    23521 cgaggtaaag ttcatttccg tacccgctct ttgcgatgaa gcggatctcc atcggtccgc
    23581 ggccggccag cgaatcaagg tggatcgtgt cggttctcca ttggttggac agcggcgcaa
    23641 aaacggtttg tgtcatcggg gcggtagcca gggcggatcc gcttcttgca tacacacgct
    23701 caccaatacc gggacaatcc gaataaaggt cgacgataag cgaatcgtta taggccacat
    23761 cgtattgcgc gtacgccacg tcaaaccaca gcgtgatcgg tgcaacggca ttggtaaaat
    23821 ccacatattg cgtaacgatc atgtcggtac cggacgagat gtaataaaaa tccgccatcg
    23881 ccgattgcga agaaagtccg tagccgccgt atgtcgtatt ccgcgaccag agacccgaag
    23941 aattctccat cgtccagttt gcaggaggga acgttgagga aacaaatcct tccgaaacag
    24001 gaggcgcggt ggtcgacgga ggcgacgatg aaagaatggt aaacgttcgc accagcgtat
    24061 cgttctgcgc attctggtcc gccccggaat tcgggctggc cgtgtacgct ttcagcgtgt
    24121 gcgttcccgg gcttaacgta tacaaaggaa gcacgatgtt cgcagttccg agcgacgcca
    24181 gcgttccgtt ccagttgtat tgctgccacg ccaggttgtc catccggtaa tgcagctcga
    24241 cattgaaaat agtatccatt cccgcattgc ggatacggag cacggggaca atggaagtat
    24301 cgcacgtagt tgacggcggg gaattcatcg cgaacacgct tgcgtcgaga ttcaccaggg
    24361 tggaaacctg caggtcgctt tcccatatcc cgcgtccgta ggttgctgca cgcagcttgc
    24421 tcgtcgggta atttatttcc agctgatcga cgataacatt cggaaggccg gtattgtacg
    24481 gctgccagtc gttaagcgtg gcgtccgtat aaaacacgcc gaggtcggtt ccgatgtaca
    24541 caatgtcgtt gcttccgttc tggtaaaccg tgcagttcac gggaatgttc ggcagcgtgc
    24601 cggaaacatt tccccaggaa gcgccgccgt cgctggaata aaaaaccttg ttgccgggag
    24661 aaaaaccgga aaacgtcacc cagagcgcat ccgggttgtt gtcgctgacc gaaatccctg
    24721 ttattgcagc agagccaaca ggaagcgtcc cggtaattgt tgtccaggtg tttccgccgt
    24781 tcgtcgtgcg ataaatattc ccgaacgtcg cagcataaat gtaattggtg ttcgccggcg
    24841 aaaccaccag cgaatacaca cacgagccgt cgaaataacc gttggaaatg ttgttccagg
    24901 tgttcccgtt gtcggtcgac ttgagcacgt ccgatgatcc catgtacatg atgttgttat
    24961 cgaccgggtc catcacgagc ggagaagtcc agtcgcaccc gaaactcgta agcgcattga
    25021 acgtatttcc cccgtctgtc gatttcagga aatatccgcc ctgcgaggaa acgaaaacga
    25081 cattgtcgtt cgtgtaatcg accagacatg tttgcccgtc cgcgccattg acctgcgtcg
    25141 ctgctgacaa tcccgtaata cgatccgttc cgttgtcctg cgcaccggca tacatgagac
    25201 tcggcgtgag aaacgacgac gacatccggt aatactgctt gatatcgatg ccgccgctga
    25261 ggtcgttcca tgtatttccc tggtcggtgg atttgaaaat ccctccgtcg ttgcaggaaa
    25321 atatcgtgtt gctgctcccc ggcaaaaatt tctgcgcgtg attgtctgcg tggcaataat
    25381 tcgtgttggg ccagcccgcc cagtcggaaa cggtgttcca cgttgttccg ccgtccgtgg
    25441 atcgcgcaat atccagaccg cccgcgaaaa gcacgtttgc attcgccggc gaagcttcga
    25501 tcaccatgtc gtaatagccg tacggtgtac agttcggcga cgggtcggtt ttctgagaaa
    25561 atgaaacacc ggcattcgaa gaataataca gcgtgaacac gttcccgcca ttctcgcacc
    25621 atacatacac cgcgttgttg tttgccggtg taactgcgat cgacgttctt ccgcccgaag
    25681 taaccccgga aatataattc cacgtcacac cggcgtcggt cgtgcgcagg aaacctgtgc
    25741 tgtcgcccgc atagacaatg ttgtcgttgc ccgggttgaa ttcgatgtcg taatattttc
    25801 cgctgcggat attattccac gtgacgcccg catccgtcgt tttgaaaatc cctgcattgg
    25861 tcgcagcgaa aagcacggaa gtgttcgagg ggtccatgat gaggcgctgg atcacaacta
    25921 cgttcgcaag cgaataattc atcgttgtcg ggttccaggt atttcctccg tctgtcgatt
    25981 tcaggatgcc cgcgctgtaa tgtccccatg tttcatattg ccagtaaatg ccgtacttgt
    26041 cgccggtggc gatgtacatg gtctgcgtat tcgtcggatc aataacgata tccgaaatgc
    26101 tcagcgaggg aagaagatct gtattcgacg accatgtcgc accgccgtcc gttgatttcc
    26161 acaatcctcc gcaggcagag ccgatccaga tgatgttgga attgttcggg tcgaacgtga
    26221 tgcagttgat gcgtcctgcg ccgccgccat tcccgggaac aacgtgcggg ccgatgaacg
    26281 accagctggc cgatgccgaa cgtgcattct ggttcgcata ctgcgttttg taaacgcggt
    26341 actggtcgaa caggatgccg ggatcgggaa aatttccgga aggatacgtg cgctgctccg
    26401 caaaccattc ccatcgtttg aattgctgcc agccgccgcc ttcctcgttt tcagattcgg
    26461 tgtcggatgg cgtgcgcccg tcccaagcgg tttcaaacgc tttctggata tcccagaatg
    26521 tcgctttttt gtccgccgga cgattatgaa ggttgggttg attgagataa tcctgtgcaa
    26581 atgcggttgc agcaaaaagg atcaccatgg acagtaaagc agagaacttt ttcaagcagc
    26641 agttttttat gtggcaattg ttacactaag ctatgaattt ggtcgcgtga ataccaatcc
    26701 ggcggtaacc cggcttccac ttaaaagcca ttacctttat gttaatgctt cgcctccttc
    26761 tcattttggc gctgctcccg ggcattctgc tcaaggcgca ggttcctttt ttcgaaacgt
    26821 attttgggtc gccgcgaccc gactatgcgc gttcgctgaa gcaatctgcc gacagcagca
    26881 tttacatcgc cggctattcg atcgatacga ttgcgggtca ttcggacgtt gctctttcga
    26941 aactttcgcc cgcaggacag cttgcctgga caaaatacta cagcgattcg ctcgacaata
    27001 attgcctttt cctccaggat gcgggagacg ggagtttttt tctgtgcggc gaaacgcaaa
    27061 cagcggccaa cgctctcgat ggtttcctca tgaaggtcga tacggccgga aatgtcctgt
    27121 ggcggaaatt gtacggcggg ccgcagaacg aatccatcaa ccgcgtggtt gttcttgcgg
    27181 acggaaagct ggcgatgacg ggcttccaga cagacgcagg cggcagcaac gggatttatg
    27241 tgcttgtcac cgacagcgcc ggcaacgaga tgtggtcggg aaattacggg ggacagaaca
    27301 acgatgccgg tcgggcggtt tgtgcgctgc ccgattcggg gtttgttgtc tttgcggaaa
    27361 cggcagtgcg ttcggcggtg aacatcgcgc tggaaggaat gcgtttcgat aaaagcggga
    27421 tcgttttgtg ggacagcatt tacgattacg gggatacact gccggaaggc tgccagggaa
    27481 tgatgctgct gtcgaacgga aatttatttg tttacggtga atcgcagcca accgccacca
    27541 cctggttcga ttttcttttc cacgagctcg atgtgaacgg caatacgatc tggtcgcgca
    27601 aaaccggcgg tccgcttccc gatgccgcgt tttccatgct cgaaacgccg ggcggattta
    27661 tcggaaccgg ttacagccgc agctattccc cgggaccgaa cgatatcatc gttttcggaa
    27721 cagacactgt gggaaccatt acctgggcgc agccttacgg cggacccggc atcgacatcg
    27781 gctacgagat catcccggca gcggcaggag gatattacat taccgcaaca ggaaatgtga
    27841 acggaaacga ccagttcggc ctgctgcaca tcgacagcgc cggctccacc gggatcgcag
    27901 gttcgcaaca ggacggagat gtccgttttt ttccaaaccc ggccagcgac cagctgacaa
    27961 tcacaacggg agctcccggg aactatacgg tgcaggtgtt ttccgccggg ggacaggaaa
    28021 tttcgtgcac acagaaaaac gggccgctgt tcgggctgga cgtttcggga cttcccgcag
    28081 gaatgttctt cgtaaaaatc tcgggggaaa acttttcacg gacggaaaag ctggtgatcc
    28141 ggtgatcagc cgacaagcgt gccgctgtcc cgcagcagtt ctgcacctac aatatcttcc
    28201 aggtcgatga tcattcctac ggaagacgcc ctgctgaaac gctccccaag gctggaaaag
    28261 taatacgcac atccgccggg atgcgctttt tcccagatca caccgaaaac ttccttccct
    28321 gacttaagct tgatcctgca gggttggtca ttgatcgtaa gaaactgatt gcgggtcatg
    28381 caacaggctt tctcaaattt caatattata acgccggaaa gccagaaaca gttgccccaa
    28441 cttattaaca gaatcggggt cttgtgaaaa gaaattaaca ggtgtccgcg tttgttaata
    28501 ttcgtggcgg cgtcttttta tgcgcctttc gtttccgcgg acgcttcggc tgcagcaaga
    28561 ccttcaagat cacgatcgaa aagataaagt ccgccttttt ctccgccgat cagcttcagt
    28621 ttgtcgagca cgcgtcgcgc aagcgcctct tcctcgatct gttcggaaac ataccactgg
    28681 agaaaattgt gtgtcgtgta atcacgctct ttcaggcagt ttgccaccag cttgttgatc
    28741 tcggcggaaa ccttcacttc gtgcttcagc acttcttcga acgcctcgtg gacggatttg
    28801 aattttttcg gtggggcggc aagagcggga accacgccgt gaccgccgcg ctcattaatg
    28861 aaacggatga gcttcagcat gtgcatgcgc tcttcgtcgg aatggtgaaa aagaaactgc
    28921 gctacgccgt tcataccctg cgtttcggcc caggaagcca tggcaagata atactgggac
    28981 gagtttcctt cgagcgtgac ctgctcgttg agtgcttttt caatttttgc ggataacatg
    29041 gctttgtcgt ttttgtcttg taaaattagg gaaaacccgc tgtttatttt tttatgactt
    29101 cacaccccgc tgtttcacag atacatttta ttttcgtttc atgaagacgg acaggattgc
    29161 tggtgatcat tgtcgtttcc gtccgcccgg cattcgttta ttttcacggt attcttaaac
    29221 caggacgaac cattcattgc tcaatctaaa ctttattccc atgaaaaaaa ctattctccg
    29281 caacacgctc gccatcgctt ccgccgcttt tcttttcgcg tcctgcgcat ctgatcacgg
    29341 actgacgatt gaaaagcgcc actaccgaaa cggctattat gtttcaaccg gcaaatcgca
    29401 tgatgtggct tcttcctcgt ccgtccgcat tgctcctgct gcaacagccc cggtgaacaa
    29461 tgaaacggtt ctgtcttccg gaaatgcgca aacggcttcc gttacagatc aaaaaacaac
    29521 gccaaccatt gcacagaaaa aaacgcccgc tgctgcatcc cgccccggcc gttcttccgt
    29581 aatactcccg gctatttttc cgcacgctgt gcacaaagca cagaaaatga attccgcgaa
    29641 aaagccggca tctgcttttc cgtcttcgga aaaaacgggg cacacggacg acaacacgtt
    29701 gctgctgatc atcctctgtt tcctgttgcc ctggctggct gtgctgctga aagagggcgg
    29761 gccgacaact tatttctgga tatcgctgct gttgtggctt tgtttctgga tcccgggcgt
    29821 catcttcgcg ctgctcgtgg tgcttgatgt gatctgacgg gggattttat tttatccctt
    29881 tccgttcgcg ccacacctgg ttgaagcttt tctgcgcaac aggcggcagc tctcttcgtt
    29941 ctccccacga tttccggaag aactggcgga gcatgaaatt tttgagcttt gcgccgccct
    30001 tgtccatttt gctgcgcttc atcatcgccc ctttccagaa gaaccacatc acggtttctg
    30061 tttttgccgc aggtccggct gtaaccgcgt cgcggcgatt gtgtgcgagc agcttgtgaa
    30121 gatcgatttt cacggggcac gcttccgaac attttccgca cccggtggat gcgtagctga
    30181 ggtggcggta ttcctgcatt cccgccatcc acggcattac cacggcgccg accgggccgc
    30241 tgtaaacggc attgtacgtg tgaccgccta cggccgtata caccgggcac gcattgaggc
    30301 atgctccgca gcgtatgcac tgcagcgcac ggcgctgttc tacctgcgcg agaagattcg
    30361 atcttccgtt atcgagaaga acaagcacga gttcttcggg tccgtcgtgc tctcccggct
    30421 gacggggccc ggtaataatg gaattataaa cagaaacctg ctggccggtt ccgtaagtgg
    30481 cgagcagcgg ccagagaaga tcgaggtcgc tgagcgcggg aatgatcttg tcgatcgacg
    30541 tgacgacgat gtgcgttttc gggaaggcca ttgccatcag cccgttgcct tcgttttctg
    30601 ttacggcaat cgcgcccgga tctgcaacga taaaattcgc gccggttatt cctgcgcctg
    30661 ccttgcggaa tttttctttc aggtggtcgc ggacccactc ggtgatctgc gccggggaaa
    30721 gatcgggcgg cgtgcctttt ttctcatgaa aaagcttcgc cacatcctcc ttcgatttgt
    30781 gcatcgccgg ggtgacgatg tgatacggcg cctcgcccgc cagctgctgg atgtattcgc
    30841 caaggtccgt ttccaccgct tcgatccctt ccttcttcag aaattcattc aggccgatct
    30901 cttccgtcac cattgatttc gatttcacca ccagctctgt tcctgatttt ttcagcacct
    30961 gcaaaatctc cctgttcgct tccgccgcat cctgcgccca gataattttt cctccgcgcc
    31021 tgatgaagtt cgcctcgaac tcgataaggt atttgtcaag attttcgatc gcgcggttcc
    31081 gcatcacgga cgctcgcgtg cgcgccagct cgaggttcgc gaactgctgt tttcccagcg
    31141 ccaccttctg gttgtaattc gcgatattga aatccagctt gcggtgaagc tccgtgtcga
    31201 acacgttctt ttccgccgat aaaatgaatt ctgctgcgcg atccatgatt tcctgttttt
    31261 atttttgggt gatcttcagc cgctcgttcc tgttcgctat ttcccatgct gtataaaata
    31321 cgagctgcgc tcttttttcc atcagcggga aatggatctt ctgcacctcg tccgtttccc
    31381 tgtgataatc cgggtgcacg ccgttaaaga aaaaagcgac ggggataccg ttcttcgcga
    31441 aattgtagtg gtcggagcga tagtaaaacc ggttggggtc gtcttttttg tcgtagcggt
    31501 aatcgagcga aagcccggtg tacgtattgt tttccgtttc gttgatcgtt ttcagctcgg
    31561 cgctgatcat gccagatccg atgacgtaaa cataattcgt gtctgcggca tgcgtcgtgt
    31621 cgatccgccc gatcatatcg atgttgaggt cgcacaccgt gctgtcgagc gggaaaaccg
    31681 ggtgcgatgt ataccacgac gaaccgagca ggcctttttc ttcacccgtc atcgtcatga
    31741 aaagaatgct tctccgcgga ccgtggcctt cttttttcgc ctgcgcaaat gctttggcga
    31801 gctcgatgat ggaaaccgtt cccgagccat cgtcatccgc gccattgtat acaacgctgt
    31861 cttttcccag gtggtcgtaa tgtgcggtta ttaccaccag ctggcctttg agatcgctgc
    31921 cttcgaggta gcccagcaca ttttccgaat gcacggaacg cccggttgac tgtgcaacaa
    31981 tgtccatttt cattttgctg aacgtgtgca cgggtttttt ctttcctgaa attttctcca
    32041 ccagcttttc cagcggcgtt tgttttcctt ccttcagaag attatccgcc atgctccgcg
    32101 tgatcagcag caccggcatc tcgtcgtaaa taacagagac gtcgttctcc tcgtcgggaa
    32161 caagcatcga catgtgatcg tgcgacatga tcttcgccag cgctttatca tactcgtcaa
    32221 ccacgatcag caatgcaccc gcgttgtgct tctgcgcttc cgacactttt ttcgcgcgct
    32281 tcacatacca ttccgacggc gctttgctgc cggaaataag attgttcccg ttccggtctt
    32341 tcggctcccc gtccatgatc agcaccgctt tcccgctcac gtcggtattt gcataatcat
    32401 tgtaggcggg gtcgtcgatg ccgtaaccga gaaaaacgag cgtgtcgcat ttttcatggc
    32461 ggctggcgtc cgaaggcgga aagaaaaaat ctttcccgaa ctcgtatttg ttcttcggtg
    32521 ttgtaacaga acccgatgag ggccggtatt cgatcagcgg aacatcctgg aaatagctgc
    32581 cgttcgcgta aggaggaata ccgaacgacg caaattgttt cgcaatgtat tcggcggctt
    32641 tcttttgtcc cggcatagcc gtttcgcggc cttcatactc atcggaagca ataacggaca
    32701 aatgcgtttt catatcctcc gccgtgatca gcgccgcgta tttttcccgc acaggatctt
    32761 ttccgccggt gaaggccagc aaaagaaagg caatgggaaa aaacagggag atttttttta
    32821 cggacatgat cgtcctgcaa atatacacgc ggcccctgca gcgtttcctg caatggcttt
    32881 cctttataaa caggcgtgct gttaattagc ggatacccgt aatgatccgc tgccggtaca
    32941 tttttccgga ctggccggta ataatgatgg tgtagatccc ggcggcaaaa tgatcctctt
    33001 caaactctac cacatttcca tccacgtttc ttttctcgat aacccttcct tctccatcga
    33061 aaacggtgta ttcaaatgaa ttttccgacg gaacgctgac gtgaaacccg gaaagcgaag
    33121 gattgggaaa aacagaaacg gcatcggctt gtgcgtattc gtcaatgccc accgtagcca
    33181 gcaggcgtgg aatgtcgttc atgatgctgt ccttgttccc gttgaaccag ccgtatttcc
    33241 ggtaaaccat tccctgcgga gtaataagat acgcgttgtt gggtgcagga ccgaacgtgt
    33301 tccagtagct gtttgccgta tcgtcgatca cgagcgggat tgaagccgga ggatgcatgc
    33361 ggataagaaa ctgcctggcg aaatgcctgc gcgttccgta tctcctgggc tgtcgcagga
    33421 ggacgctgtc ctgcagattg ctctgtgtta tccatacgct ccccgaatac ggacttacat
    33481 ccggcgtatc aggatgcggc tcgactacat aaacaagcaa aaagttgacg gaattgtgat
    33541 aggctatgta aatatccggc agcacgtacg tcatgctgtg gcgggaaagg ggacaggtga
    33601 gcgaaacagt aataagaagc agcggtttgt tttgggcgag tatcgttgtc agctgctgtg
    33661 caacgccggc ggtatcgtac aaacgaaaat cggaaacaac ctggccgggc tgataacccg
    33721 ttgtcgcaaa attgtttgcc atggccgaag catatattcc gaagggcgcg atatgtgccg
    33781 aatcgggcga cagggttcca tagccggtaa aagatgtgat atcgctctgc gcatgaagcg
    33841 cggagcctcc gaaaaccaac agcaacagta agaaacgcct gaacataagg cgtctaagtt
    33901 agcttttttg acttattaaa aactccttaa aatcctcaaa aatcagttat gacagggaat
    33961 cttgtttacc cggtttgcat ggcgtccgcc ttcaaaaggc gttgaaagga agatatctac
    34021 gcatctcagg gcatcttcat ccgaaataaa gcgggcgggc agcgaaagaa tattggcatc
    34081 attatgcatg cgggccagcc cggcgatctc gggtttccag cacacagctg cccgtatacc
    34141 tgcatgcttg ttggccgcca tgcttatgcc gttaccggat ccacatataa gtataccgaa
    34201 gtcggcttcc ccgttttcca cagcattcgc taccgggtga gcgaaatcgg gataatcgac
    34261 actttccccg gaatccgttc caaagttcct ggtgctgatt ccgttagcag aaaggtgccg
    34321 gatgataacc tcttttaatt tatacccggc atgatcacag ccgatggcca gccttacctt
    34381 tgtcattgga ctttttttgc aaagttaagc ggataaaaaa cgcgtttccc ggggaatgta
    34441 aaatagaaac caacaatgca aggactgtca gcaaaaaaca acactggaaa taaaccagtt
    34501 atctgtaact ttattaacat gctttttgtt gaaataagaa cgcgcctgtt attaaaaagg
    34561 tcacaaaata acttggcaga tccggaccat tttccgggta atgaattctt ttttccgtgc
    34621 aagcattgtt gaaaaaatag aacaggcaaa gaactaaccg tttattcacg ttcatttttg
    34681 atgctggcaa ttaacaatgg cgtgaaaaaa caaaacctga aaaatgttga aaagaacaat
    34741 caatgctgtt tttcaggtca ttgaaaaaat tcccgctgtt gaaatcgtgt tacctgtttt
    34801 tgagaacaaa caatattgtc cgtgaacgca aggttatcaa ccgtttcaac agtataatag
    34861 tagtagtaga ttttatttaa aaaatatata tgaatactat agaatacgac gtcgatgaaa
    34921 aggtgagccg gcttgatccg ggcctggatc ttttttacga gatcgggaaa ctgaaaaaag
    34981 aaaagaatgc tgtcctgctg gcgcattatt accaggagaa tgacatccag gatgtggcgg
    35041 atttcatcgg cgacagcctt ggactggccc agcaggccgc caaaacgaaa gcggacatca
    35101 ttgtttttgc cggcgttcat ttcatggcgg aaacagcgaa gatcctcaac ccgcacaaga
    35161 aagttatcct cccggatctc cgcgccggct gctcgctggc ggatacatgt ccggcagaca
    35221 ggttttcaga attccgcaaa cgttttcctg atcacgtggt catttcgtac atcaactgca
    35281 gtgcggaagt gaaagcgctg agcgacatca tctgcacgtc gagca
//
LOCUS       DASTSF010000437         3007 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_153559,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000437 DASTSF010000000
VERSION     DASTSF010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3007
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153559"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..926)
                     /locus_tag="VFU15_12760"
     CDS             complement(<1..926)
                     /locus_tag="VFU15_12760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008505110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEU4718704.1"
                     /translation="MEKTHEKVWDSCLNIIRDNVSSQSFKTWFEPIKPLKISGNVLTI
                     QVPSQFFYEWLEEHYITLLKKTIRKELGTEGKLEYSIIMENAFSNTKPYTVKIPTSNR
                     REVKNPAVAMPIDLNKNTIRNPFIIPGLKKVNVESQLNPNYSFDNFVEGDCNRLARSA
                     GWAVAEKPGGTAFNPLLIYGGVGLGKTHLAHAIGLQIKYKHPGKTVLYVSSEKFTHQF
                     IDAVKNNNQNDFIHFYQMIDVLIIDDVQFFAGKDKTQDVFFHIFNHLHQSGKQLVLTA
                     DKPPVEMQGMEQRLLSRFKWGLSADLQVPSLET"
     gene            complement(1145..2086)
                     /locus_tag="VFU15_12765"
     CDS             complement(1145..2086)
                     /locus_tag="VFU15_12765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017730445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase"
                     /protein_id="HEU4718705.1"
                     /translation="MKQNRRDFLKAVSGAASLLGLGAFTSSFRKAGKDNRVRLVILHT
                     NDVHSHIDPFPDTDPKYPGLGGVERRAALISQVRREEENVLLLDAGDIFQGTPYFNMY
                     GGEAEMKLMTAMGYEASAIGNHDFDGGLDNLAKQLEHASFPLLCANYDFTGTPMEGKT
                     QPYKVFSKGGLSVGVFGLGIELAGLVDSRLYGKTLYLDPIVKAAEMVKVLKDEKKCDV
                     VICLSHLGYKYPNDKVSDVVLAQKSRDIDVIIGGHTHTFLDKPVVIKNREQKDVAVVQ
                     VGWAGIRLGKIIIHTDKKAKKKSVETASIEVRRNPSI"
     gene            complement(2073..2849)
                     /locus_tag="VFU15_12770"
     CDS             complement(2073..2849)
                     /locus_tag="VFU15_12770"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF014871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase"
                     /protein_id="HEU4718706.1"
                     /translation="MRFTRFVFPALFPAAILASACHPPAHVTQVDAQYVQMDAKTDQH
                     DSVTAAIIAPYRVSMEKEMDEVLVKSDSAMTKDGTTESKLGNFVADVCLTVTNDSFGT
                     NGHPAADICLFNNGGLRSSLPAGNITRGNIFELMPFDNEIVILTLSGQKMWQLIRYVC
                     ATGGEPEAGMKLGIRPDKTPGSVLIGGVPFDSTRTYKVLTSDYLANGGDKMNFFSAPL
                     DMQTTGYRIRDALINYCVNENKKGHTLSSRLDGRIYYETK"
BASE COUNT          639 a          738 c          808 g          822 t
ORIGIN      
        1 gtttcgaggc tcgggacctg gaggtcggcg gaaagtcccc acttgaaacg ggagagcagg
       61 cgctgttcca ttccctgcat ttcaacgggc ggcttgtcgg cagtaagcac gagctgtttt
      121 ccgctctggt gaagatggtt gaagatgtgg aagaaaacgt cctgtgtttt gtctttaccg
      181 gcgaagaact gcacatcgtc aatgatgagc acgtcgatca tctggtaaaa gtggatgaag
      241 tcgttctggt tgttgttctt gacggcgtcg atgaactggt gcgtgaattt ttccgaggaa
      301 acgtacagca cagttttgcc gggatgcttg tatttgatct gcaggccgat cgcatgcgcg
      361 aggtgtgttt tgccaagacc tactccgccg taaatgagaa gcgggttgaa agcggtgccg
      421 cccggttttt cggcaacagc ccatccggca gagcgcgcga ggcggttgca gtcgccttcg
      481 acgaaattgt caaaggagta attcgggttg agctgcgact cgacgtttac ttttttcagg
      541 cccgggatga tgaacgggtt gcggatcgta ttcttgttca gatcgatcgg catggcgacc
      601 gccgggtttt ttacttcgcg gcgattggat gtggggatct ttacggtgta aggcttcgtg
      661 ttgctgaagg cgttttccat gatgatggaa tattccagct tgccttcggt accgagttcc
      721 ttgcggatcg ttttcttgag aagtgtgatg taatgctctt cgagccattc ataaaagaac
      781 tggctcggaa cctggatggt cagaacgttg ccggaaattt tgagaggctt gatcggttcg
      841 aaccaggttt tgaaactttg agaactgaca ttgtccctga taatgtttaa gcagctgtcc
      901 cagacttttt cgtgtgtttt ctccattcgt aaattctcct gcaattatat tgtgaggtgt
      961 gtgtttttta ttcgtgtccc aaaattatta tttcgaaggt agcgcacaag attttccaac
     1021 aaaaaaaatt tggaaaaaat ttcgcggagg gcttgacttt gtcgatccgt caacagttag
     1081 ggctttcagc gcatcggttt gtgtttttct atctaaaaaa atgacttgtg caaataaaag
     1141 cgcattaaat cgacggattt cttctgactt cgatcgaagc tgtttccacg gatttttttt
     1201 tcgctttttt atccgtgtga ataatgattt ttcccaaacg tattcctgcc cagccaacct
     1261 gcacgacagc gacatctttc tgctcgcggt ttttgatcac gaccggtttg tcaagaaatg
     1321 tgtgcgtgtg tccgccgatg atcacgtcga tgtctctcga cttctgcgcg agcaccacat
     1381 cgctcacttt gtcgttcggg tatttgtatc cgagatgtga aagacagatc accacgtcgc
     1441 attttttttc atccttgagc accttcacca tttcagccgc ctttacaata gggtcgagat
     1501 acagcgtttt tccataaaga cggctgtcca ccaacccggc cagctcgata ccgagtccga
     1561 acacaccgac gctgagtccg cccttgctga aaactttata cggctgcgtt tttccttcca
     1621 tcggtgtgcc ggtgaaatcg taattcgcgc agagcagcgg gaacgatgca tgctccagct
     1681 gtttcgcgag attgtcgagt cccccgtcga aatcatggtt gccgatcgcg gacgcttcgt
     1741 atcccatcgc cgtcatcagc ttcatctccg cttcgcctcc gtacatgttg aaatacggcg
     1801 tgccctggaa aatatctcct gcgtcgagga gcagcacgtt ctcttcttcc ctgcgcacct
     1861 ggctgatgag cgctgcgcgc cgttccactc cgccgagccc gggatatttc ggatcggtgt
     1921 cggggaacgg atcgatatga ctgtgcacgt cgttcgtgtg aagaatgaca agacggacgc
     1981 ggttgtcttt tcccgctttc cggaacgacg aggtgaatgc gccgagcccg agcagcgatg
     2041 ctgcaccgga aaccgctttg aggaaatcgc gcctattttg tttcatagta gatcctcccg
     2101 tcgaggcggc tgctgagtgt gtgacctttt ttgttttcat tcacgcagta attgatgagt
     2161 gcgtcgcgta tgcggtagcc ggtggtctgc atgtccagcg gcgcggaaaa gaaattcatt
     2221 ttgtctccgc cgttcgcgag gtaatccgac gtaagcactt tgtacgtgcg agtggaatcg
     2281 aacggaacgc cgccgatgag cacgctgccc ggtgttttat ccgggcggat gccgagcttc
     2341 attcccgctt ccggttctcc gcccgttgcg caaacgtacc tgatcagctg ccacattttc
     2401 tggccggaga gggtaaggat gacgatctcg ttatcgaacg gcatcagctc gaaaatattg
     2461 ccgcgcgtga tgttcccggc cggaagcgaa gagcggagcc cgccgttgtt gaacaggcag
     2521 atgtcggcag cgggatgacc gttcgttcca aagctgtcgt tggtcaccgt gaggcatacg
     2581 tcggcgacaa aatttcccag cttcgattcc gtcgtcccgt ctttagtcat cgcggaatcg
     2641 cttttcacca gcacttcatc catttctttt tccatggaga cgcggtaagg tgcgatgatc
     2701 gcggcagtaa ccgaatcatg ctgatccgtt ttcgcatcca tctgcacata ttgcgcgtcc
     2761 acctgcgtga cgtgtgcggg aggatgacag gcagaggcaa gaatagctgc cgggaaaagg
     2821 gccgggaaaa caaaccttgt aaagcgcatg ggactattac aagtttaccc aataaaagcg
     2881 agatatgtgt ttgtgcagcg ctttttttct gaatttagca cgaacgcaaa ttcttaacaa
     2941 tcccgcatgt tcattgcaga aacttccttg cgcgtacggt acggcgaaac agaccggatg
     3001 ggttatt
//
LOCUS       DASTSF010000438         6932 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_567945,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000438 DASTSF010000000
VERSION     DASTSF010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 6932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6932
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_567945"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(320..1642)
                     /locus_tag="VFU15_12775"
     CDS             complement(320..1642)
                     /locus_tag="VFU15_12775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009583638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEU4718707.1"
                     /translation="MEKKIFVLDTSVIIYDHNAIKNFKEHDVVVPITVLEELDNFKKG
                     NDTKNFAAREFTRYIDKISGRNTLQDWVNINGKGHGMFKIEMNMSTKIDAEKIFGERK
                     ADHRIINSALFLSETNPGRKVVLVTKDINLRIKAKSLNLAAEDYETGKIKNPNDLYTG
                     ASEMERVSAASVSELYEKGFTTTQSVLKKTKALANHYYILKSGQKSVLARHNPVTHLM
                     ERVKKEMAFHITPRNAEQTFALDAILRPDIRLVTIQGVAGTGKTLLSLAGALEQRRNY
                     HQIYLARPIVPLSNKDIGYLPGDIKSKLNPYMEPLWDNLKYIKSQFAEKDKEFKQINE
                     MVENEKLVVCPLAYIRGRSLSNVFFIVDEAQNLTPHEVKTIISRAGENSKIVFTGDIF
                     QIDTPYLDTQSNGLSYMIDRLKGKELYAHITLHRGERSELANLANDYL"
     gene            1948..>6932
                     /locus_tag="VFU15_12780"
     CDS             1948..>6932
                     /locus_tag="VFU15_12780"
                     /inference="COORDINATES: protein motif:HMM:NF040138.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HEU4718708.1"
                     /translation="MTRKFYSAISFSALLLLCGNLHSQTAVWKQPMDHPEMSFYQIQQ
                     QFNHDMRFKQREIEREQRRESGQQTSAGKGVSGQQGAESEEDMESYVLFKRWEDFMAP
                     RVYPSGDITLPSTTWDRFQDYLNSNPAAMGQYQQGNGNSNQRTANPQNSIMTSTWTFA
                     GPTGAPSGAGPGGGGAGRVNFLRFDPTNTNILWVGAPAGGLWKSTNGGSSWTTNTDFL
                     TVVGATDVVIDPTNTQVMYLATGDGDAGDTYSIGVMKSTDGGSTWNTTGLNWTVNQGR
                     VISKLLINPSNPQIIMAFSNAGIWRTTNGGTSWTQVSTASVKDGEFMPGTPNTVYACG
                     TRFYKSTDGGVTWTNITSGLPANTSVDRMAIAVTAANSAYIYMVAGSAANDGFYGFYK
                     STNSGTSFTQVTVSSPSNILGWASAGNDTGGQGWYDLSIDADPTNANAVIVGGVNVWR
                     TTNGGTSWTLNAQWTGSGAPFIHADVHSLNYLPGSGSTYFATSDGGVFKTTNTGGAWS
                     DLSNNLCIAQPYRIGLSGSNANIWITGHQDNGTNIKNGATYGVTMGGDGMDCFIDRTN
                     NNVMYGEQYQGSLNRTTNGGASWSGITSGLSGTAPWVTPWYQDPSVANTIYCGRTNLF
                     KSTNQGTSWSQLTALPASPSGTIVDFKVAPSNNQVIYVARATGLFKTTNGGTSWTTVT
                     GTLPVGSASISRVDVKPNDPNTVYVTFSGYSSGNKVFKTSNGGTSWTNISTGLPNLPV
                     NCVRFDPSGSATEAVYVGCDVGVYYMDNSFTSWQPYFTALPNAPVYDLEIYAATGKLR
                     AATYGRGVWETDIYNPGTLPPVAQFTANTQIVCAGNPVQFTDQSSFNPTSWSWTFQGG
                     SPATSTSQNPTVTYSAPGTYSVSLTATNANGSNTNNQVSYITVLGSQSPPLVEGFEAA
                     TFLPTGWTAKNINNDGIFWAQASVGYNSTKSAMFDNYNNDVAGAQDEMWAPRLDCSSY
                     STLSLTFDVAYAPYDLVTYCDSLEVLVSTDCGTTWNSVYLKGGSTLETVAPQTTAFTP
                     TNAQWRNDAVNLNTYAGQSSVLIVFRNRGHYGNILYVDNINITGTAAAIPNGNFTASV
                     TSACAGTPVNFTDASSGTPTSWTWTFPSGTPASSTSQNVTGVVWNTAGTYTVTHTATN
                     ASGTGTTTQTITILPSPTVTITPSGTSVCSGNSVTLTGGGASTYAWMPGSLSGSPVTV
                     TPASTTTYTLTGTAANGCSSTATQAITVNATPTVTATASPATVCSGSSSSLTGSGAST
                     YSWMPGSLSGSPVSVTPGSTTTYTVTGTAANGCTNTATRTVTVNPTPTVTATASQGTI
                     CNGSSTTLTASGATSYTWMPGSLSGTSVSVSPASTTTYTVTGTSGTCSNTATLTITVN
                     PSPTVTTTASPATICSGSSSTLTASGATSYTWMPGSLSGSPVSVTPGSTTTYTVTGTA
                     ANGCTNTATRTVTVNPTPTVTATASSSSICTGNSVTLSATGATTYNWMPGNHNGQPWT
                     TTPASTTTYTVTGTSGGCSSTSTVTVIVGAPPVVTASASNPSICTGGSSTLTGSGAAT
                     YTWMPGSLSGTSVSVSPTSNTTYTVTGSSGPGCSSTATVSVSVNPLPTVTASASSTTI
                     CNGDSTVLTGSGASTYSWMPGNLSGSSVTVNPTSTTTYTVTGTSASGCSATA"
BASE COUNT         1666 a         1922 c         1758 g         1586 t
ORIGIN      
        1 cgggtcgatc gtccatttgg ttttcatggt actatgtttt tgagtttgtt gtttcgtttg
       61 aaagacagaa caaagatatg tattttatat gtatatacac atattgtaat ggcaaatatt
      121 ttattgtgtt gatttatagc attttaatgt ggatcgaaaa cctcttatgt tgccggtaaa
      181 atgaggattt ccgctgggat gtttttcaat ggaaaagtcc cgcaatggaa acgatgagcc
      241 tgaaaagaga gctgaaaaac cggctgtggc gcattacaac accggaagca aaaagcttcc
      301 ggcgggatca taaccgcggt tacaaataat cgttcgccag gttcgccagc tcgctccgtt
      361 cacccctgtg cagggtgatg tgcgcataca gctcttttcc tttgagacga tcgatcatgt
      421 acgaaagtcc gttcgactgc gtatcgaggt acggcgtatc gatctggaaa atatcgccgg
      481 tgaaaacgat cttggaattt tctcccgcac ggctgatgat ggttttcact tcgtgcggtg
      541 taagattttg cgcttcgtcc acgatgaaga acacgttcga aagacttctt ccgcgtatgt
      601 aggcgagcgg gcacacaacg agcttttcat tttcaaccat ctcgttgatc tgcttgaact
      661 ccttgtcctt ttccgcgaac tggctcttga tgtatttgag attatcccac agcggttcca
      721 tgtacggatt cagcttcgat ttgatgtcac cgggaagata gccgatgtct ttgttgctca
      781 gcggaacgat ggggcgcgcg aggtagatct ggtggtaatt tcttctttgc tccagcgcac
      841 cggccaggga aagaagcgtt tttcccgttc ctgcaacgcc ctgtatcgtc accagccgga
      901 tgtcgggacg caatatggcg tcgagcgcaa acgtctgctc ggcattacgc ggtgtgatgt
      961 gaaaagccat ttcctttttc acccgttcca tcaggtgcgt caccgggttg tgccgtgcga
     1021 gaacggattt ctggccgctc ttcaggatat aatagtgatt ggcgagcgcc ttcgtttttt
     1081 tcagtacgga ttgcgtcgtc gtaaatcctt tttcatacag ttcgctcacc gatgccgcgg
     1141 aaacccgctc catctccgat gcccccgtat acaggtcatt cgggtttttg atctttcccg
     1201 tttcgtaatc ttccgccgca aggttgagcg atttcgcttt gatccgaagg ttgatgtctt
     1261 tcgtgaccag caccactttc cttccgggat tcgtttccga aaggaaaagc gccgagttga
     1321 tgatgcggtg atccgctttc cgttccccga aaattttttc cgcgtcgatc tttgtcgaca
     1381 tgttcatctc gatcttgaac atgccgtggc ctttgccgtt gatgttcacc caatcctgca
     1441 gcgtattgcg gccggaaatt ttatcaatat agcgggtgaa ttcgcgcgcc gcgaaattct
     1501 ttgtgtcgtt tccctttttg aaattgtcca gctcttcgag gacggtgatc ggaactacta
     1561 catcatgttc cttgaaattt ttaattgcgt tgtggtcata gataatcaca gacgtgtcaa
     1621 gcacgaaaat ttttttctcc atcgccatat aaaagagcgt attagttgag ctaaactata
     1681 aaaacctaaa agcgttgttc cgcgtattaa agtcatttaa tgaacagttc tttgttatct
     1741 gttttgaaca ataccggaat ttgctttcgc cgataaaatc tcaatactcg tcgaaagtgc
     1801 ttgattttta gcaaaataaa gcgtaaactt gagattgccg gggttattat ctgtttgatt
     1861 ttattttgac cggctttttg caattcatga tcggggctat ggaaaaattt ttttccattc
     1921 cctatttaaa atacaaagcc acacaccatg accaggaaat tttactcagc gatttctttc
     1981 tccgctctcc tgctgctctg cggaaatctt cattcccaaa cggccgtctg gaaacagccg
     2041 atggatcatc ccgagatgag cttttaccag atccagcagc agttcaatca tgacatgcgc
     2101 ttcaagcagc gcgagatcga acgggaacag cggcgcgagt cgggacagca gaccagcgca
     2161 ggcaagggcg tgagcgggca gcagggcgcg gaaagcgaag aggacatgga aagctatgtg
     2221 cttttcaagc gctgggaaga tttcatggcg ccgcgcgtgt atccttccgg cgacattaca
     2281 cttccttcaa caacgtggga tcgcttccag gattacctca acagcaatcc cgccgctatg
     2341 ggccagtacc agcagggaaa cggcaacagc aatcagcgca cagcgaatcc gcagaattcg
     2401 atcatgacca gcacatggac tttcgccgga cccacaggag ctccttcggg cgcaggtccg
     2461 ggcggtggtg gcgcaggccg cgtgaatttt ctgcgtttcg atccgacgaa cacgaatatt
     2521 ctctgggtgg gcgctcccgc aggcggactg tggaagtcaa ccaacggcgg ctccagctgg
     2581 acaacaaaca cggattttct caccgtggtc ggcgcaacgg atgttgttat tgatcccacg
     2641 aatacgcagg tcatgtacct cgccacaggc gacggcgatg cgggcgacac ctattcaatc
     2701 ggtgtgatga aatcgacaga cggaggaagc acgtggaata caaccgggct taactggacg
     2761 gtcaaccagg gacgtgtgat cagcaagctg ctcatcaatc cgtcgaaccc gcagatcatc
     2821 atggcattct cgaatgcggg gatctggaga acgaccaacg gcggcacttc gtggacacag
     2881 gtttcgacgg cttccgtgaa agacggagaa ttcatgccgg gaacgccgaa cacggtttac
     2941 gcctgcggaa cgcgttttta caagtcaacc gacggcggcg ttacgtggac gaacatcacg
     3001 tcaggtcttc cggcgaacac ttccgttgac cgcatggcca ttgccgtaac agcagccaac
     3061 agcgcataca tctacatggt ggccggcagc gctgcgaacg acggcttcta cggcttttac
     3121 aaatcgacca actcaggaac ttcattcacg caggtgacgg tttcttctcc ctcgaacatt
     3181 ctcggctggg ccagcgcagg aaacgacacc ggcggacaag ggtggtacga tctttccatt
     3241 gacgccgatc cgaccaatgc gaatgcggtc atcgtcggcg gagtgaatgt ctggcgcacc
     3301 accaatggcg gaacctcgtg gacactcaat gcacagtgga ccggaagcgg tgctccgttc
     3361 attcatgcgg acgtgcatag cctgaattat cttcccggga gcggcagcac ttatttcgcc
     3421 accagcgacg gcggcgtttt caaaacgaca aacacgggcg gtgcgtggag cgatctcagc
     3481 aacaacctgt gcattgcgca gccttaccgc atcgggcttt cgggttcaaa tgcaaatatc
     3541 tggatcaccg gccaccagga caacggaacc aatatcaaaa atggcgcaac atacggcgtc
     3601 acaatgggcg gcgacggcat ggactgcttc atcgacagga cgaacaacaa cgtcatgtat
     3661 ggagagcaat accagggttc gcttaacagg acgacgaacg gaggggcgtc atggtccggc
     3721 atcacatccg gcctgtcggg aacagcaccg tgggtaacgc cgtggtacca ggatccgtct
     3781 gtcgccaaca cgatttactg cggaagaacg aaccttttca aatcaaccaa ccagggaaca
     3841 agctggtcgc agctcactgc gctcccggct tctccgtcgg gaacaatcgt ggatttcaaa
     3901 gtagcgccat cgaacaacca ggtgatctac gttgcccgcg caaccggttt gtttaaaacc
     3961 accaacgggg gaacgtcgtg gaccacggtt acgggaacac ttccggtggg aagcgcatct
     4021 atttcccgcg ttgatgtaaa acccaatgat ccgaatacgg tttacgtcac attctccggc
     4081 tattcttcgg gcaataaagt tttcaaaacc tccaacggag gaacgtcgtg gacgaatatt
     4141 tccaccgggc tgcccaacct tcctgtcaac tgcgttcgtt ttgatccttc gggttccgcg
     4201 acagaagccg tttacgtcgg ttgcgatgtc ggcgtttatt atatggataa cagctttaca
     4261 tcgtggcagc cgtattttac ggcgctgccg aatgcgcctg tttatgatct tgaaatttat
     4321 gccgcaacag gaaagcttcg tgctgcaaca tacggccgcg gagtgtggga aaccgacatt
     4381 tacaacccgg gaacgcttcc gcctgtggcg cagttcacag ccaatacgca gatcgtctgc
     4441 gcaggaaatc ctgtgcagtt caccgatcag tcttccttca atcccacatc ctggagctgg
     4501 acattccagg gcggatctcc ggccacatcc acgtcccaga acccgaccgt gacttattcg
     4561 gcacccggaa cttacagcgt atcgctgacg gccacgaacg cgaacggaag caacacgaat
     4621 aaccaggttt cctacatcac ggttctcggt tcacaatctc cgccgctggt ggaaggcttt
     4681 gaagcagcca cattcctgcc gacaggctgg acggcaaaga acatcaacaa cgacgggatt
     4741 ttctgggcgc aggcaagtgt cggctacaac agcacgaaga gtgcgatgtt cgacaactac
     4801 aacaacgacg tggccggtgc acaggatgaa atgtgggccc cgcgcctcga ctgcagcagc
     4861 tacagtacac tttcattgac attcgatgtg gcgtatgctc catacgatct cgtcacttat
     4921 tgcgactcgc tcgaagtgct tgtttccacc gactgcggaa ccacgtggaa ttccgtttac
     4981 ctgaaaggcg gctccacgct tgaaactgtc gccccgcaga caaccgcatt tacaccgacg
     5041 aacgcacagt ggagaaatga cgcggtgaac ctgaacactt acgccgggca aagcagtgtg
     5101 cttattgttt tccgcaatcg cggtcactat ggaaatatcc tgtacgtcga taatatcaac
     5161 atcacaggaa cggctgcggc aattcccaac ggcaacttca cggcgagcgt aaccagcgca
     5221 tgcgccggaa cgcctgtcaa tttcacggac gcctcttcgg gaactcctac aagctggaca
     5281 tggacattcc ccagcggaac gccagcgtct tccacttccc agaacgtaac gggcgtggtc
     5341 tggaatacgg caggaacgta taccgtcacg cacacggcaa caaatgcttc cggtaccgga
     5401 acgaccacgc aaacaattac tattcttccc tcgcccaccg ttacgatcac gccttcggga
     5461 acatctgtct gttcaggtaa cagcgttacg cttacgggag gaggtgcatc aacttatgca
     5521 tggatgccgg gaagcctgag cggttctccg gtcaccgtta cgccggccag cacaacaaca
     5581 tatactttga cgggcacggc ggcgaacgga tgttcttcca ctgctacgca agctattact
     5641 gttaatgcta ctcccacagt gacggcaacc gcatcaccgg caactgtgtg ttccggaagc
     5701 agcagctcac tgaccggcag cggagcctca acgtattcct ggatgccggg aagtctttcc
     5761 ggttcccccg tatctgtcac tcccggatct acgaccacat acacagtgac gggaacagcg
     5821 gccaacggct gtacgaatac agcaacaaga actgtaactg ttaacccgac accgaccgta
     5881 acagctaccg cttcacaggg aacgatctgc aacggaagca gcactacgct tacggcaagc
     5941 ggagcaacgt cctacacctg gatgcccgga agcttgtcag gaacatccgt ttccgtttct
     6001 ccggcatcga caacaactta cacggtgacg ggtacatcgg gaacctgttc gaacacagct
     6061 acgctgacca tcacggtaaa tccgtcaccc acggtaacca caaccgcatc gcccgctaca
     6121 atctgctcgg gaagcagttc tacgcttacc gcgagcggag caacgtccta cacctggatg
     6181 cccggaagtc tttccggttc cccggtttcc gttacgcccg gatctacaac gacgtacacg
     6241 gtgacaggaa cggctgccaa tggttgcacg aatacggcaa caagaacggt tacggtaaac
     6301 ccgacaccaa cggtaacagc cacagcctcc agctcttcga tctgcaccgg aaacagtgta
     6361 acactgagcg caacgggcgc aaccacctat aactggatgc cgggtaacca taacggccag
     6421 ccgtggacga caaccccggc aagcaccaca acttataccg tcacaggaac cagcggcgga
     6481 tgttcttcca catctaccgt taccgttata gtcggcgcac cgccggtggt caccgcttct
     6541 gcttcgaatc cctctatctg cacaggaggc agctcgacac tcactggttc gggagccgca
     6601 acctatacgt ggatgccggg aagtctttca ggaacgtctg tttcggtttc accgacatcg
     6661 aatacaacgt atactgtgac cggctccagc ggcccgggat gctcgagcac ggcgactgtt
     6721 tcagtaagcg tgaatcccct gccgaccgta acagcttccg cttccagcac tacgatctgt
     6781 aatggtgaca gcacggtgct caccggaagt ggcgcttcaa cttacagctg gatgcccgga
     6841 aatctttccg gatcatctgt aacggtaaac ccgacttcca caacaaccta taccgtcacc
     6901 ggaacaagcg ccagcggatg ttcggcgacc gc
//
LOCUS       DASTSF010000439         2429 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_511037,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000439 DASTSF010000000
VERSION     DASTSF010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2429
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_511037"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1925
                     /locus_tag="VFU15_12785"
     CDS             <1..1925
                     /locus_tag="VFU15_12785"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEU4718709.1"
                     /translation="EWVLLRDYNCDGKMDIFCYASSGSGGIRVYKNVSTIAGGLQFQL
                     VDPLIMSDYYPNSSPPPEALWVTSVDIPAIRDIDHDGDMDVLTYDASGVHVEFHRNMS
                     EEMGYGCDSLIYKLQTGCWGDFAEDPFNSHISLNDTCSPPQIAGWTGNSVSPYRMHNG
                     SCMECINTDGDNDQDILIGDITNPYIVYGRNGGDSSFAKMDFTDPTYPSYDTPLNMNM
                     FNAAFHLDVNNDGNKDVIFAPNAPNSSANFTSAWYYRNTGTTDSVVLTYVQDDFLQDN
                     MIDVGEGAYPRFFDYDGDGDMDMFIGNYGYYASATTYPSEIAFYENTGSASSPSFTLV
                     TRDFLDLHANSTGIICPVPTFGDVDGDGDMDMIVGDASGKLTYFRKDPGPNNNFVLVQ
                     TNYMGIDVGNNAAPQLVDVDRDGLPDLLIGEQSGNVNYYRNIGTATAPNFALVNNLFG
                     GFFVNITGNITGYSVPWLWDNNGQYELLVGSERGYLYRFDNIDQNLSGNFTLTDSMYV
                     DSYLGLRISPCVTFLNGDTIPDLVIGNYAGGVGVYLGAAGVGIEEHDLHHPFFSVYPD
                     PAQNSLTIEIEQEQELSLPATFSIYSVNGQLLKEEKINSRKTDVDISMLAPGFYIATV
                     ETAGGRISRQKFVVRN"
     gene            complement(1988..>2429)
                     /locus_tag="VFU15_12790"
     CDS             complement(1988..>2429)
                     /locus_tag="VFU15_12790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718710.1"
                     /translation="DGYKEDFDVDSTTVSVTGKYQFWNFFVGPYYSFPMNKLTIDLRF
                     VAGLVHASTPEFKVDLEDQMNATFYQRSSTSNAFGFQAGAGLRYQLAGGLCAKLNADY
                     YYAHPDFMITNDNRVNSAGRLLTEYKEPVSMINLNIGLAYQFGK"
BASE COUNT          584 a          680 c          608 g          557 t
ORIGIN      
        1 acgaatgggt gctgctgcgc gattacaact gcgacggcaa gatggatatt ttctgttacg
       61 cctcgagcgg cagcggcggg atccgcgtgt acaaaaacgt ttccacgatc gcaggcggat
      121 tgcagttcca gctcgtggac ccgctgatca tgtcagatta ttacccgaac agttcgcctc
      181 cgccagaagc gctctgggtg acgagcgtcg atattcccgc gatccgcgac atcgatcacg
      241 acggcgacat ggatgttctt acgtacgatg caagcggcgt gcacgttgaa ttccaccgca
      301 acatgagcga agaaatgggc tacggatgcg acagtcttat ctacaagctg caaaccggct
      361 gctggggcga ttttgccgaa gatccgttca actcgcacat ttcgcttaat gatacctgct
      421 cgccgccaca gatcgcgggg tggacaggaa attccgtttc accttaccgc atgcacaacg
      481 gctcctgcat ggaatgcatc aacacggacg gcgacaacga ccaggatatt ctcatcggcg
      541 acatcacgaa cccgtacatc gtttatggcc gcaacggcgg cgacagctct tttgcaaaaa
      601 tggatttcac cgatccgact tatccttcct atgacacgcc attgaacatg aacatgttca
      661 acgcagcctt ccacctcgac gtaaacaacg acgggaacaa agacgtgatc ttcgctccga
      721 atgcgccgaa ctcttctgca aatttcacca gcgcgtggta ttaccgcaac accggcacca
      781 ccgattctgt tgtgctcact tatgtgcagg atgatttcct ccaggataac atgatcgacg
      841 tgggcgaagg agcgtatccg cgtttctttg attacgacgg cgatggcgac atggacatgt
      901 tcatcgggaa ttacggctat tacgcaagcg ccaccacgta tccttcggaa atcgcgttct
      961 atgaaaatac aggcagcgcg tcgtctcctt cgtttaccct ggtgacgcgc gatttcctgg
     1021 accttcacgc caactcaacc ggcatcatct gccctgttcc tacattcggc gacgtcgacg
     1081 gggacggcga tatggacatg atcgtcggcg atgcatcggg caaactcacg tacttccgga
     1141 aagacccggg accgaataac aatttcgtgc tcgtgcaaac gaattacatg ggtatcgacg
     1201 tcggcaacaa cgcagcaccg cagctggtgg acgtcgatcg cgacggattg cccgacctgc
     1261 tgatcggcga gcaatcgggc aatgtgaatt attaccgcaa catcggaaca gcgacggcgc
     1321 cgaattttgc gctcgtgaac aatctcttcg gcggattttt cgtcaacatc accggcaaca
     1381 tcacgggcta cagcgttccc tggctgtggg ataataacgg gcaatacgag ctgctggtcg
     1441 gttccgagcg cggctatctt taccgcttcg acaacatcga ccagaatctc tccggcaatt
     1501 tcacgctcac tgattcgatg tacgtcgatt cctatctcgg gctgcgcatt tctccgtgcg
     1561 tgacattcct gaacggcgac acgatcccgg accttgttat tggaaattat gccggcggcg
     1621 tgggcgttta tctcggcgca gcgggcgtcg gcatcgaaga gcatgacctg caccatccct
     1681 tcttctcggt ttatcccgat cccgcacaga attcgcttac gatcgaaata gaacaggagc
     1741 aggagctttc ccttcccgcc acgttcagca tttattccgt gaacggccag ctgctgaaag
     1801 aggaaaaaat aaattcacgc aaaacggacg ttgacatctc gatgcttgcc ccgggatttt
     1861 atatcgcgac agtcgaaaca gccggcggca ggatttcccg gcagaaattc gttgtaagga
     1921 actgatattt taaacaaaaa gccccgccgg ttacggcggg gccttctgcc aaaaccacca
     1981 cgcgttttta ttttccgaac tggtaagcga gaccgatgtt cagattaatc attgaaacgg
     2041 gctctttgta ttcggtgagt aatcttcccg ctgaattcac acggttatcg ttcgtgatca
     2101 taaaatccgg gtgtgcgtag taataatccg cattcagctt tgcgcacaat cctcctgcaa
     2161 gctggtatct cagccccgcg ccagcctgaa agccgaacgc atttgaagtg gatgaccgct
     2221 ggtaaaatgt cgcattcatc tggtcttcca ggtccacctt gaattccggt gtcgaagcgt
     2281 gcacgaggcc tgcgacaaag cgcagatcga tcgtcagctt gttcattgga aaagaataat
     2341 acggacccac aaaaaagttc cagaactggt atttgccggt aacggaaaca gtagtgctgt
     2401 ccacatcaaa atcttccttg tacccatcc
//
LOCUS       DASTSF010000440         5402 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1404908,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000440 DASTSF010000000
VERSION     DASTSF010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5402)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5402
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1404908"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1813
                     /locus_tag="VFU15_12795"
     CDS             <1..1813
                     /locus_tag="VFU15_12795"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HEU4718711.1"
                     /translation="TPVTVSTSATSSTVCAGTTTTITASGATNYNWQPGNLSGSSVTV
                     SPSSATTYTVIGTSGTNCADTTTISIAVNPSPSVTVTPSSSSLCNGGSVTLNATGAST
                     YEWQPVNQTGSSVTDSPSSTTTYTLVGTDGNGCTDTVMTTVNVGTSPTVTATASSMST
                     CNGSSVTLTGNGASTYAWQPGSLTGTNVSVSPSANTTYTVVGTNAAGCTDSTTIDITV
                     NPLPVITLASANDTVCAGASATLSASGANTWSWSSGGTNASETVTPSSTTTYTVSGTD
                     ANGCTGTATATVVVNALPAISVSGNTTLCVGDNTTLSASGASAWSWSTGSTSSSVNVT
                     PPAGTTTYTVTGTNASGCSDSATVTVTVNPPPTANITGSSTTCMGSPVSLTATGGGTY
                     AWSSGGNSATEIVTPSSTTTYSVIVSVGTCADTATFTVSVNAPPAADAGSDQTITLGA
                     SAQLSGSGGTAYTWSPSTGLSCTNCQNPDASPTVTTVYYVTVVDANGCTAMDSVTVSV
                     DDGCGAAVFVPNIFSPNADGSNDVFKVYGGCWKEYHLVVYDRWGEVVFESTNPADAWD
                     GSYKGKMLDPAVFVYVLTGTSLTGTEIDQKGNVSLVK"
     gene            1834..2880
                     /locus_tag="VFU15_12800"
     CDS             1834..2880
                     /locus_tag="VFU15_12800"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HEU4718712.1"
                     /translation="MKTGITQIFFTAALTLAAFSVNAQDIHYSMFNETPVMLNPARAG
                     VPFSTRIILNYKDQWQSVASPYKTMAFSGDVSLFKSDKRKAYMGLGLNVFNDKAGDVG
                     MKTTQVGLSASAILAPDANNKISIGLMGGFMQRSIGTGNQQWGSQYDGTAFNPNLASG
                     ENMATQNYNLPDAGVGINWYYGKGEKYMTANDGVKLDFGVAMFHPHGPKYSFYGDANE
                     RLDPKIVAHGWASFGTGNSNLCIEPSFLFVMQGPQKELTPGVNLKYILSESSKYTGRK
                     KASAFSLGGYMRTKDALILTTMFEYSSYAAGFSYDLNTSTLKTVSHARGGMEFFIRFV
                     FPNPFGSDTSKSMI"
     gene            2905..4443
                     /locus_tag="VFU15_12805"
     CDS             2905..4443
                     /locus_tag="VFU15_12805"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEU4718713.1"
                     /translation="MILLLLVVAGSSPLAAQEDSVKTTITNLGSDINTAFEEYTPVIS
                     ADGSIMLFTSRRPVTDKEKKKNKESKETVYLSRFNSQAGKWEAPSALPSPINVEGRHT
                     SVLGLSNDGQRMLIYRDDETGNGNIWESHLDGLQWSEPELLPDPINSPYHESSASFSP
                     DGRTLYFVSDRPGGLGGRDIWISTLGADGKWSTPINPGAAINTPDDEEGVFIHPDGVT
                     LYFSSKGRGGLGGYDVYKSVRVNGMWTTAVSLGQPINTTGDDLFFVLTASGKKAYYSC
                     NAQADNKGKRDLYEITFTPVDKKVADRGPRLTLLKGVITDSLTHQPLQATIQVFDNEK
                     NELVAQVNSNSASGQYLLSLPSGKNYNITVSANNYLFHSENFNIPDTSSYHEVQKNIA
                     MQKIEVGNKMVLNNIFFDYNKATLRPESYAELNNLVNLMKANPTLEIEISGHTDNRGT
                     ADYNKKLSQDRAKAVVDYLVSQGIDEKRLTYVGYGFDKPIATNDTDEGRQLNRRVEFK
                     VLKK"
     gene            4542..>5402
                     /locus_tag="VFU15_12810"
     CDS             4542..>5402
                     /locus_tag="VFU15_12810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718714.1"
                     /translation="MKKTVSLAALLLITLRISAMGTDSLHAPYFLQVLRMDGATVKRT
                     ADFTEGLQEPSKHSIRSADPGRSAHISLLTRETLVLRAVDTGDDSLMNLPGNFIFVFE
                     SSDLEDSVRFLPLISDSTTATHFLERMGKVKKREWEEYRILIRKKGSVTWYALYVRFQ
                     SGLLSVNIPNKSNRQTMAMKSISPALPVIHLGHIDYTHYSFGPGISFNIGLQPTQPLH
                     RLLADIVGPVSVEWMFYPISDINAIFAIHSSAIGLFFNSGYGLFHWGIAWYTPKYCRA
                     EAYIGINLVPA"
BASE COUNT         1319 a         1621 c         1394 g         1068 t
ORIGIN      
        1 cacgccggtc accgtttcca cttccgcaac atcttccacg gtctgcgcag gaacgacaac
       61 aacgatcacg gcttccggcg caacgaacta taactggcag cccgggaatt tatcgggatc
      121 ttctgtcact gtttctccgt cttctgcaac aacgtacacg gtcatcggaa caagcggcac
      181 caattgcgcg gacacgacga ctatttccat tgcggtcaat ccttcgcctt ccgttaccgt
      241 cacaccttcg tcttcttcgc tctgcaatgg aggaagcgtt acgctgaatg caaccggcgc
      301 ttccacgtat gaatggcagc cggtcaatca gaccggaagc tcggtcacgg attccccgtc
      361 ttccaccaca acgtacacgc tggtgggaac ggacggaaac ggatgcacgg atacggtgat
      421 gacaaccgtg aacgttggca caagtccgac ggtgacagca accgccagct ctatgtcaac
      481 ctgcaacgga agctccgtca cgcttaccgg caacggcgcg tccacgtatg catggcaacc
      541 cggaagtctt acgggaacga acgtttccgt ttctccttct gcgaatacga cctacactgt
      601 cgtcggaaca aacgcagcgg gctgcaccga ttcaactacg atcgacatca cggtcaaccc
      661 gctcccggtg atcacgctcg cttcagcgaa cgacaccgtg tgcgcgggcg cttccgcaac
      721 actctccgct tccggcgcga acacctggag ctggtcgagc ggcggaacga acgcaagtga
      781 aaccgtcaca ccttcttcca ccacgactta caccgtgagc ggaacggatg ccaacggttg
      841 cacgggaaca gcaacagcca ctgttgtggt gaacgcactg cccgccattt ccgtttccgg
      901 caacacgaca ttatgcgtgg gcgacaacac aacactgtca gcttccggcg cttcggcgtg
      961 gtcgtggagc accggctcga cttcttcttc cgtaaacgtt acgccgccag cgggcactac
     1021 gacgtatacc gtaacaggaa caaacgcaag cggatgttct gattccgcca cagtgacggt
     1081 cacggtaaat ccgccgccga cagcgaacat caccggcagt tccaccacat gcatgggttc
     1141 cccggtttcg ctgacagcca caggcggcgg cacgtatgcg tggagctccg gcggaaattc
     1201 agcgacggaa attgtcacgc catcttccac cacaacgtat tccgtcatcg tctctgtcgg
     1261 aacatgcgcc gacaccgcga cgttcaccgt cagcgtaaac gcaccgcccg cggctgacgc
     1321 cggcagcgac cagaccatca cgctgggagc ttccgcgcag ctgagcggat caggcggaac
     1381 ggcttatacg tggtcgcctt caacaggatt gagctgcacg aactgccaga accccgacgc
     1441 atcgcctacg gtaaccacgg tttattatgt caccgtcgtc gacgcgaacg gatgcacagc
     1501 gatggattcc gtcacggtca gcgttgatga cggctgcggc gctgcggtct tcgtcccgaa
     1561 tattttctcg ccgaacgccg acggcagcaa cgatgtcttc aaagtgtacg gcggatgctg
     1621 gaaagaatac catctcgtgg tctacgatcg ctggggcgaa gttgttttcg aatccactaa
     1681 cccggccgac gcatgggacg gttcctacaa aggaaaaatg ctggatcctg ccgtgttcgt
     1741 ctatgttctt accgggacat cactcaccgg gacggagatc gatcagaaag gcaacgtcag
     1801 tctcgtgaaa taaattttta aaaaatccat tccatgaaaa caggaatcac gcaaatattt
     1861 ttcacggctg cgctaacact tgccgcattt tccgtgaacg cgcaggacat tcactattcc
     1921 atgttcaatg aaacgcccgt gatgctgaat cccgcacgcg ccggcgttcc gttcagcacg
     1981 cgcattatcc tcaattacaa agaccagtgg cagtctgttg cgtctccgta caaaacgatg
     2041 gcgttcagcg gcgacgtttc gcttttcaaa agcgacaagc gcaaagcgta catgggcctc
     2101 gggctgaatg tcttcaacga taaagcaggc gatgtgggaa tgaaaaccac gcaggtggga
     2161 ctttccgctt ccgcgatcct tgcgccggac gcgaacaata aaatttccat cggcctgatg
     2221 ggcggattca tgcagcgcag catcggtacc ggtaaccagc aatggggcag ccagtacgac
     2281 ggcacggcgt tcaatcccaa tcttgcgagc ggcgagaaca tggccacgca gaattacaac
     2341 ctcccggacg caggcgtcgg catcaactgg tattatggaa aaggagaaaa gtacatgacc
     2401 gccaacgacg gcgtgaagct cgatttcggc gtcgcgatgt tccatccgca cggaccgaaa
     2461 tattctttct acggtgatgc gaacgagcgt ctcgatccga agatcgtcgc gcacggatgg
     2521 gcttccttcg gaaccggcaa cagcaacctc tgcatcgagc cttctttcct tttcgtgatg
     2581 cagggtccgc agaaagagct cacgcccggt gtcaacctga aatacattct cagcgaatca
     2641 tccaaataca cgggccgcaa aaaagcgtct gcgttttctc tcggcggcta catgcgtacg
     2701 aaagatgcgc tcatcctcac gacgatgttc gaatactcga gctacgcagc cgggttcagc
     2761 tacgacctca acacttcgac tttaaaaacc gtcagtcacg cgcgtggcgg aatggaattt
     2821 tttatccgct tcgtcttccc gaacccgttc ggatccgaca cgagcaaatc catgatctga
     2881 tccatgtccg gttcccgttt tcttttgatt ctcctcctcc tcgtcgtcgc cggcagttcc
     2941 ccgctggcgg cgcaggagga ttctgtaaag accaccatca ccaatctcgg cagcgatatc
     3001 aacaccgcgt tcgaagaata cacgccggtg atctccgccg acggaagcat catgctgttc
     3061 acgtcgcgcc gcccggtgac agacaaagaa aaaaagaaga acaaggaaag caaggaaacc
     3121 gtttacctct cgcgcttcaa cagccaggca ggaaaatggg aagccccttc cgcacttccg
     3181 tcaccgatca acgtggaagg acgtcatacg tccgtgctcg ggctttccaa cgacgggcag
     3241 cgcatgctca tttaccgcga cgatgaaacc ggcaacggga acatctggga aagtcatctc
     3301 gacggtttgc agtggtcgga accggagctt ctccccgacc cgatcaattc accttaccac
     3361 gaatcgagcg cgagcttttc gccggacgga agaacactgt atttcgtcag cgaccgtccc
     3421 ggcggattgg gcggacgcga catctggatc agcacactcg gcgcagacgg aaaatggagc
     3481 acgcccataa atcccggcgc agcgatcaac acgcccgatg acgaagaagg tgttttcatt
     3541 caccccgacg gtgtgacgct ttacttcagc tcgaaaggaa gaggcggtct cggcgggtac
     3601 gacgtgtaca aatccgtacg cgtgaatggc atgtggacaa ccgcggtcag cctgggccaa
     3661 ccgatcaaca ccacgggcga cgatctgttc ttcgtgctca cggccagcgg taaaaaagcc
     3721 tattacagct gcaacgcgca ggcggataat aaaggcaaac gcgacctgta cgagatcacg
     3781 ttcacgcccg ttgataaaaa agttgccgac cgcggaccga ggctgacgct gctgaaaggc
     3841 gtcatcaccg attcgctcac gcaccagccc ctgcaggcga cgatacaggt attcgacaac
     3901 gaaaagaacg agctcgtcgc gcaggtgaat tccaacagcg cgagcgggca atatcttctt
     3961 tcgctgccgt cgggaaaaaa ttacaacatc accgtttcgg cgaacaacta tcttttccat
     4021 tccgagaatt tcaatattcc cgatacgtcg tcgtaccacg aagtgcagaa gaacattgcg
     4081 atgcagaaga tcgaagtggg aaacaagatg gtgctgaaca atattttctt cgattacaac
     4141 aaagccacgt tgcggccgga atcgtacgcc gagctgaaca acctcgtcaa cctgatgaag
     4201 gcgaatccca cgctcgaaat agaaatttca ggacatacgg ataaccgcgg aacagccgat
     4261 tacaacaaaa aactttcgca ggaccgcgcg aaggccgttg tcgattacct cgtcagccag
     4321 gggatcgatg agaagcggct gacgtacgtc ggctacggct tcgacaagcc catcgccacg
     4381 aacgacaccg acgaaggaag acagctgaac cgccgggtgg aattcaaagt gctgaagaaa
     4441 taaatttccc tttcttaaca acgcacggtt aaaacatccg ttccccgcaa cgggaacggc
     4501 aggcgcgcct ggcctaattt cattgtccgg atcattccac aatgaaaaaa accgtttcgc
     4561 tcgccgccct tctgctcatc acgctgcgca tttcagcgat gggaacagat tcgctgcatg
     4621 caccttattt cctccaggtg ctgcgcatgg acggggcgac cgtaaaacgc acggcggatt
     4681 tcacggaagg actgcaggaa ccgtccaaac attcgatacg aagcgcggat cccggcagga
     4741 gcgcacatat ttcgctgctt acacgtgaaa cgctcgtgct ccgcgccgtg gatacgggcg
     4801 acgattcgct gatgaacctc cccgggaatt tcatctttgt tttcgaaagc agcgacctgg
     4861 aagacagcgt gcggttcctt ccgctcatca gcgattccac aacggcaaca catttcctgg
     4921 aacggatggg caaagtaaaa aaacgcgagt gggaagagta ccgcatcctc atccggaaaa
     4981 aaggaagcgt tacgtggtac gcgctgtatg tgcgcttcca gtcgggcctg ctttcggtga
     5041 acatcccgaa caaatcgaac aggcagacca tggcgatgaa atccatttca ccggcgcttc
     5101 ccgtcataca cctcgggcac atcgactaca cgcattattc attcgggccc ggcatcagct
     5161 tcaacatcgg gcttcaaccc acgcagccgc ttcaccgcct gctcgccgac atcgtcggac
     5221 cggtgagcgt ggaatggatg ttctacccga tcagcgacat caacgccatc ttcgcgatcc
     5281 attcttccgc gatcggcttg ttcttcaact ccggctacgg acttttccat tggggaatcg
     5341 cgtggtacac gccgaaatac tgccgtgcgg aagcctacat cgggatcaat ctcgtcccgg
     5401 cg
//
LOCUS       DASTSF010000441         5699 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1405390,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000441 DASTSF010000000
VERSION     DASTSF010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5699
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1405390"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..241)
                     /locus_tag="VFU15_12815"
     CDS             complement(<1..241)
                     /locus_tag="VFU15_12815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718715.1"
                     /translation="METKSDELGLALQQMQTNLRQITEDHRERIWLQSGKSTLGEKLR
                     GDKPLQELAKDIIDFIVSYSDSQIGTMYVLREDVLR"
     gene            884..1714
                     /locus_tag="VFU15_12820"
     CDS             884..1714
                     /locus_tag="VFU15_12820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018477526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEU4718716.1"
                     /translation="MTALAQKNKPAPQDGGKPLVIGEVQELLSAELGEKRTLNIYLPQ
                     SYAQNDTAKYPVIYLLDGGMDEDFLHIAGLVQFSSFPWVARVPESIVVGIVNTDRKRD
                     FTFPTTIEEDRKKYPTAGHSDKFIAFLEKELQPFIEKKYRTTASKTIIGESLGGLLAT
                     EILLKKPALFNKYIIVSPSLWWDNGSLLKQESDIFTENFTQQTDVYIGVGKEGLTPGD
                     PPHVMEVDANQLADRLKNSKSKSLDVCFDYLPQEDHATVMHQAVLDAFRMLYPAEDKK
                     "
     gene            2010..2636
                     /locus_tag="VFU15_12825"
     CDS             2010..2636
                     /locus_tag="VFU15_12825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014680405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEU4718717.1"
                     /translation="MPVNWPDAMKPLLKKYKGKPHPLDYKNIYQLLVVVLLSAQDSDK
                     HINQLSPAFFEAFPDMKSLARADEQALFPFVRSIRNSGNKIKWLMQLSRTVKDDKKIP
                     LTMEGLTALPGIGRKSANVIMRGAGVKAEGVIVDLHVVRVAPRIGIAKGTDPKKIEQQ
                     IMDVIPQKDWGEIGMAISFLGRETCRPSNPKHEECVVKKFCAFYKENG"
     gene            2670..2885
                     /locus_tag="VFU15_12830"
     CDS             2670..2885
                     /locus_tag="VFU15_12830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718718.1"
                     /translation="MDQSQRFEIIRADITKLSVDVIVNAANTSLLGGGLDEAPHAYKD
                     IHTVMASQVDLVDVVGSFTPRIVRMDG"
     gene            complement(2901..4298)
                     /locus_tag="VFU15_12835"
     CDS             complement(2901..4298)
                     /locus_tag="VFU15_12835"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEU4718719.1"
                     /translation="MTFPGVKKFTGNLHTFFIPEKYRSDEETFRKCKVFINTTLITTI
                     FAIFFLGNAVLFHMPMATIAMICCSVLFFSFAWMLRAGVPFRVCTNAYIALGVIATAW
                     DTYWAGGLNSIHTPWFVFPAIGTLLIGTIRESRFWLVVSLAILGAFGVGALRGYPFPN
                     ELGPDYYSWMQLSAYAGLIVILFIVVLVVENAYQKSLHRREAALESLRKSQVQLIHQE
                     KMASLGQLIAGIAHEIQNPLNFVNNFSFVSKDMLDDLREAKTEEERNDLIRLLSENLD
                     RIEQHGKRADGIVKRMLMHSRTQPGERQETDINRLCDEAIALAFQNARVNNPDFKSAI
                     EKNYSEQPVVVNAVPQEISRVILNLLDNAFYSVNRKRMEKAAAGEAYQPSVSVTTAAG
                     NKSVSFAIRDNGDGVAPGILGNIFQPFFTTKPAKEGTGLGLSICYDIVKAHGGEIRAE
                     STEHEGTTMTVTLVM"
     gene            complement(4295..4981)
                     /locus_tag="VFU15_12840"
     CDS             complement(4295..4981)
                     /locus_tag="VFU15_12840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718720.1"
                     /translation="MTIFEYVKEPHPWIDLSHYTLTQVILFFTGSMLWLVCYIDTLID
                     IKKKKTINIPVGCVITNYGWEISACLFFVPDMGTLLVAAYWAWMLFDTFIFASTFRYG
                     HKQMMVPFFKRNAHIFILLGIVISLSTQATYMVKGFDIPMAPISGDIINLYMSAAFLY
                     LLFIPGYEGNSMVTAWSKFLGTGIINYMFYTKYPGNYFLVAIGISIALLDITYIVLLR
                     KKNNQTLVLT"
     gene            complement(4997..>5699)
                     /locus_tag="VFU15_12845"
     CDS             complement(4997..>5699)
                     /locus_tag="VFU15_12845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002692825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phytoene dehydrogenase"
                     /protein_id="HEU4718721.1"
                     /translation="NEEVNINKGHVIYSDSEWALTSISQLQFWSGNTFNVANHYNGKI
                     KTILSVDISGWHSPGLNGKIADECSLEEIRDEVWAQLKKSLNVGGKTVLRDEQIEFWF
                     IDRDICETGTVPKTKNKEPLLVNHTNSWTLRPSAHTFIPNLFLASDYVKTFTDLATME
                     GANEAARRAVNSIIDVSGAKADLCKLWNLHEPDFLAPFRLHDEKRYHKGLPWKNEFSG
                     IETMMSEIKAKMHKV"
BASE COUNT         1304 a         1498 c         1570 g         1327 t
ORIGIN      
        1 gccggagcac atcttcccgg agcacgtaca tcgttccgat ctgcgaatca gaatacgaca
       61 caatgaaatc gataatgtcc tttgccagct cctgcagcgg cttgtcgccg cgaagctttt
      121 caccaagcgt gcttttgcct gattgcagcc agatacgctc ccggtgatct tcggtgattt
      181 gcctgagatt ggtctgcatc tgctgcaatg caagacccag ttcatctgat tttgtttcca
      241 taaggtgtgc gtctggttaa ttggcgggtg gacaataggc cgggataacg ggcagcgcgt
      301 gcaaaagcat tcgggaaaat gcgtgcgcac cagctcgccg tccatttttt atcgatcgtt
      361 gcgggaacca accccttcat aagcgtgcgg ccattctgtg cattaaaaaa cccactgcaa
      421 acacagcacc ggctgagcaa aagcccggaa ctgcatttac cgtgggtctt attttgaaat
      481 ttcgccgtcc tttcttcgtt cgcattcact gttgcgggaa accagtgagc gcgtagtcgt
      541 cagacatact gaaggaaaag gcttcttttc agcgtaattt cattcaggct aatttaaact
      601 ttttttaaaa atgacgatga attaccggaa taacggtggt atttcccggc aaaaccatat
      661 acggcggaag ttccgtgcga ctgtgtcctg gtgaccgttt ttccctgttc ccggaactgc
      721 atgcgagact ggcgcgatga taaaaacacg gcaggccttg ctgattttaa taccttagga
      781 aaacaaaacg gcgccgcgca tgatcgcctc cggccggagc tgtttcacaa ccacattagt
      841 tatgaaaaca ctcctggcca ttttctgcat cgccgctttt tcaatgacgg cactggcgca
      901 aaaaaacaaa ccggctcctc aggacggcgg caaaccgctc gtgatcggcg aggtacagga
      961 gctgctgtcc gcagagctgg gagaaaaacg gacgctgaac atttacctgc cgcaaagcta
     1021 tgcgcagaac gacacagcaa aatatcctgt catttatctc ctcgacggag gcatggacga
     1081 ggattttctg catatcgccg ggctcgtgca gttcagcagc ttcccgtggg tggcgcgcgt
     1141 gcccgaatcc atcgtcgtgg gcattgtcaa caccgaccgg aaaagggatt tcacctttcc
     1201 caccaccatc gaggaagaca ggaaaaaata cccgacagcg ggacattccg acaagttcat
     1261 cgcattcctc gaaaaagagc tgcagccgtt cattgaaaag aaatacagga caaccgcatc
     1321 gaaaacgatc atcggcgaat cgctcggcgg actgcttgcc acggagatct tattgaaaaa
     1381 acctgcgctg ttcaataaat acattatcgt gagcccgagc ctgtggtggg acaatggatc
     1441 gctgctgaaa caggaatcgg atattttcac ggaaaatttt acgcagcaaa cggatgttta
     1501 catcggcgtc gggaaagaag gactgacgcc cggcgatccc ccgcacgtga tggaagtgga
     1561 tgcgaaccag ctcgcggatc gtctcaaaaa ttcgaaaagc aaaagcctgg atgtctgttt
     1621 cgattacctg ccgcaggaag atcacgcaac ggtgatgcac caggcggtgc ttgatgcgtt
     1681 caggatgctg tatccggcag aagacaaaaa gtaggtttca tgccacgcac tgtttccacg
     1741 cagctttttg atgccgctgc cgtgctggtg caaaaaaaaa aaaccgggat cagtccgggg
     1801 caggctgtgt gtcgtccggg agttgaatgg tctccgcttt tcctttgtgt gctatgtgaa
     1861 agttctttgt gttcaccgtg gttaaaaacg gttctccgtt tgtcgttctc tccggatcgc
     1921 tttcaggatg ggtaaaacaa tcggccgttt cccggacgag agttccacaa ttccgtcgta
     1981 attttccggt ccatcaaaaa ccgattccca tgcctgtcaa ttggcccgac gcgatgaagc
     2041 ccctgctgaa aaaatacaaa ggcaaaccgc acccgctcga ctataaaaac atttaccagc
     2101 tgctcgtcgt cgtgctgctc tccgcgcagg attccgataa acacatcaac cagctttcac
     2161 cggccttctt cgaagcgttc ccggacatga aatcgcttgc acgcgccgat gaacaggcgc
     2221 tgtttccgtt cgtgcgcagc atccgcaact ccggcaacaa gatcaaatgg ctcatgcagc
     2281 tttcacgcac ggtgaaagac gacaagaaaa ttccgctcac aatggaagga ctcaccgcgc
     2341 tgcccggcat cggaagaaaa tctgcgaacg tgatcatgcg cggagcaggc gtaaaagccg
     2401 aaggcgtgat cgtcgacctg cacgtggtgc gcgtggcgcc gcgcatcggc attgcgaaag
     2461 gaaccgatcc gaaaaagatc gagcagcaga tcatggatgt tatcccgcag aaagactggg
     2521 gcgagatcgg catggccatt tctttcctcg gccgcgagac ctgccgtcct tccaacccga
     2581 agcacgaaga atgcgtcgtg aagaagttct gcgcgtttta taaagagaac gggtaacccc
     2641 gggcaacatt ttttcgcacc atccgacgaa tggatcaatc gcagcgtttc gaaattatcc
     2701 gtgccgacat cacgaaactt tctgtcgacg tgatcgtgaa tgcggcgaac acttccctgc
     2761 tcggcggcgg attggatgaa gcgccgcatg catacaaaga cattcacacc gtgatggcgt
     2821 cgcaggtaga tctcgtggat gttgtcgggt ctttcacccc gcgcatcgtg cgcatggacg
     2881 ggtgaacgct gaacagattt ttacatcacc agcgtcaccg tcatcgtcgt tccttcgtgt
     2941 tccgtgcttt cggcgcggat ctcgcccccg tgcgctttca cgatgtcgta gcagatgctc
     3001 agcccgagac cggttccttc tttcgccggt ttggtggtga agaacggctg gaaaatattg
     3061 ccgaggatac cgggtgctac gccgtcgccg ttgtcgcgga tggcgaacga aacggattta
     3121 tttcctgcag cggtcgtaac ggaaacagaa ggctggtacg cttcaccggc tgccgctttc
     3181 tccatgcgtt tccggttcac cgaatagaac gcattgtcga gcaggttcag gataacgcgt
     3241 gaaatttcct gcggaacggc gttcacgacg acaggctgtt cgctgtaatt cttttcgatc
     3301 gcgcttttga aatcaggatt attgacgcgc gcattctgga aagccagcgc gatcgcttcg
     3361 tcgcagaggc ggttgatgtc ggtttcctgc cgctctccgg gctgtgtgcg gctgtgcatg
     3421 agcatccgct tcacgatgcc gtcggcgcgt ttgccgtgct gttcgatgcg gtccaggttt
     3481 tcgctgagca gcctgatgag gtcgttgcgt tcttcttccg ttttcgcttc gcgcaggtcg
     3541 tcgagcatgt ctttcgacac gaatgaaaaa ttattcacga agttgagcgg gttctggatc
     3601 tcgtgcgcga tgccggcgat gagctgcccg agcgatgcca tcttctcctg gtgaatgagc
     3661 tgcacctggc ttttgcgcag cgattccagc gcggcttccc ggcggtgcag gcttttctgg
     3721 tacgcatttt ccaccacgag cacgacgatg aacaggatca cgatcagccc ggcgtaggcg
     3781 ctcagctgca tccagctgta gtaatcagga ccgagttcat tgggaaacgg gtacccgcgc
     3841 agcgcgccca caccgaacgc gccgagaatg gcgagcgaga cgaccagcca gaaacggctt
     3901 tcgcggatgg tgccgatgag cagcgtgcct atggcgggaa aaacgaacca gggtgtgtga
     3961 atgctgttga gcccgccggc ccagtacgtg tcccatgccg tcgcaatcac gccgagcgcg
     4021 atataggcgt tcgtgcatac gcggaacggt acgcccgcgc gcagcatcca agcgaaagag
     4081 aaaaaaagaa cgctgcagca gatcatcgcg atcgtcgcca tcggcatgtg gaaaaggacc
     4141 gcgttgccga ggaagaaaat ggcgaagatc gtcgtgatca gcgtcgtgtt gatgaagacc
     4201 ttgcatttcc ggaacgtctc ttcatccgac cggtattttt ccgggatgaa aaaggtgtga
     4261 agattacctg tgaatttttt taccccggga aatgtcatgt caggacgagt gtttggttgt
     4321 tcttttttcg gagcagcaca atgtaggtga tgtcgagcag cgcgatcgat atcccgatgg
     4381 ccacgaggaa ataattgccg gggtatttcg tatagaacat gtagttgatg atgccggtgc
     4441 cgagaaattt gctccacgcc gtcaccatcg aattgccttc gtaaccggga atgaaaagca
     4501 ggtaaaggaa ggccgccgac atgtagaggt tgatgatgtc gccggagatc ggcgccatcg
     4561 ggatgtcgaa gcctttcacc atgtaggtcg cctgcgtgga aagtgaaatg acaatgccga
     4621 ggagaatgaa gatgtgcgcg ttccgtttga agaacggcac catcatctgc ttgtggccgt
     4681 acctgaacgt cgaggcgaag atgaacgtgt cgaacagcat ccacgcccag tacgcagcga
     4741 caagcagcgt gcccatgtcg gggacgaaaa aaaggcaggc ggaaatttcc cacccgtaat
     4801 tcgtgatcac gcagccgacg ggaatgttga tcgtcttttt ctttttgatg tcgatcagcg
     4861 tgtcgatgta gcacacgagc cacagcatgg agccggtgaa gaaaaggatc acctgcgtga
     4921 gcgtataatg gctcaggtcg atccagggat ggggctcctt aacatattcg aagatcgtca
     4981 tgggcggacg gtgtcgttac actttgtgca ttttcgcttt gatctccgac atcattgttt
     5041 cgatgccgct gaattcattt ttccacggaa ggcctttgtg gtaacgcttc tcgtcgtgga
     5101 ggcggaaagg tgcgaggaaa tcgggctcgt gcaggttcca tagtttgcag agatccgctt
     5161 tcgcgcccga aacgtcgatg atggaattga ctgcgcggcg cgccgcttcg ttcgcacctt
     5221 ccatcgtggc gagatcggtg aacgttttca cgtaatcgga agcgaggaat aaattcggga
     5281 tgaacgtgtg cgccgacgga cgcagcgtcc agctgttggt atgattgaca agcagcggtt
     5341 ccttgttctt cgttttcggc actgtccctg tttcgcagat gtcgcggtcg atgaaccaga
     5401 attcgatctg ctcgtcgcgg agaacggttt tccctcccac gttcaggctt ttcttcagct
     5461 gcgcccacac ttcgtccctg atctcttcga gcgaacattc atcggcgatc ttcccgttca
     5521 gtcccggcga atgccagccg gaaatgtcga ccgagagaat ggtttttatt ttcccgttgt
     5581 aatggttcgc gacattgaag gtgttgccgg accagaactg cagctgcgag atcgacgtca
     5641 gcgcccattc gctgtcgctg tagatcacgt ggcctttgtt gatgttcacc tcttcgttc
//
LOCUS       DASTSF010000442         3225 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_376441,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000442 DASTSF010000000
VERSION     DASTSF010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3225
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_376441"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..87)
                     /locus_tag="VFU15_12850"
     CDS             complement(<1..87)
                     /locus_tag="VFU15_12850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015812879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HEU4718722.1"
                     /translation="MQLQNDLILKAARGEKTERTPVWLMRQAG"
     gene            complement(249..467)
                     /locus_tag="VFU15_12855"
     CDS             complement(249..467)
                     /locus_tag="VFU15_12855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718723.1"
                     /translation="MFFCCTTSRRVLTWPPDVLLPFPAPWQPDCERVCGSPGAPVLVE
                     VPPAFTGSVGVPGGHKRKKTGARQSGKE"
     gene            827..2050
                     /gene="lhgO"
                     /locus_tag="VFU15_12860"
     CDS             827..2050
                     /gene="lhgO"
                     /locus_tag="VFU15_12860"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HEU4718724.1"
                     /translation="MDTEKKTFDVIVVGGGCVGLAAAYKINLRHPHLKVAVLEKEDHV
                     SPHQTGHNSGVIHSGIYYKPGSYKAKNCVEGRREIVAFAKEHKIAHDICGKIIVATHE
                     SELAHMNKVYNNGVANGVEDIELIDAKKIKEIEPYAEGIAGIWVGCTGIIDFPGVTKK
                     LAELLEQKFPGSKVFTSTEAKDFVHKNESTDIITNRGTFSARWLVTCAGLQSDRIAKK
                     EGAKPEAAIVGFRGDYYDLTEKGMKKVRNLIYPVPNPKYPFLGVHFTRMVHGGVECGP
                     NAVFVFKREGYGKTSFSLRDTAQALGFGGTWKFFAKNMKFGWDEYRGAFSKRFFLKRL
                     RTLIPSLEADDIVSSRSGVRAMALSPEGKMIDDFSIEFHGNAIHVLNAPSPAATACLA
                     IGKAIEEMATEKFGL"
     gene            complement(2189..2965)
                     /locus_tag="VFU15_12865"
     CDS             complement(2189..2965)
                     /locus_tag="VFU15_12865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718725.1"
                     /translation="MIFFRRFISVFAFCVPAFLDCAAQDGAVKYPPGLMHDLHVQEVS
                     EVQYTFSPAGGQLPPWTKNTIDFDTSGRIVKVQRFDPMKSTAISSEIYHYGPGGKISR
                     VDFYSYGMPENSCFENYIYDTTGRLVLRRDSSMQSGKITGFAVFTYDSSGLVQTKDYY
                     LKKKKLQDRFIYTSDSAGRLVKDVSYYYLESDKPIDMHVYFYEDGRLTGEDVYLHDMK
                     VSCNRVGYVYDGNGKLTSYNFFDGKGKLLSRTDIVYTYYR"
     gene            complement(3014..>3225)
                     /locus_tag="VFU15_12870"
     CDS             complement(3014..>3225)
                     /locus_tag="VFU15_12870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718726.1"
                     /translation="ALQKKKGKARPVQALVLACSITCAVFVVLLLQSGTKVNHEEYVY
                     FSWTNFSAIALCWVMMSFFSIRGAE"
BASE COUNT          830 a          866 c          824 g          705 t
ORIGIN      
        1 cccggcctgg cgcatgagcc atacaggcgt gcgttctgtt ttttcccccc gcgccgcttt
       61 caggataaga tcgttttgca gttgcatgcg gcaaaggtac ttttcggttc cggatttcag
      121 gtttcagatt tcggatttgg aatgtacatc cgcgaaatcc cgcggttcac ctcatttcat
      181 ttttcacgga caacctcctg ccgggattgc gcatcaggag gaaaaccata ctgatggaac
      241 gaaaaatgct actctttccc gctttggcgg gccccggtct ttttgcgctt atgtccgccg
      301 ggtacgccta ccgacccggt gaatgcgggc ggaacttcaa cgagcaccgg cgcaccggga
      361 gagccgcata cgcgctcaca atcgggttgc cacggcgcag gaaacggcag cagcacatcg
      421 ggcggccatg tgagcacgcg gcggctcgtc gtacagcaga agaacatcaa aaaaaatatt
      481 ttcaccctgc gaattcattc gtggatcatc cactgctttt cacaaaccca aacttgcaga
      541 caccgcacgt taaacacacc cgtcaagatg aaaacaaaac ttctttttct tttttgcgtg
      601 agtgatggga gcggcatcct tttgcgcagc aaaagataca gcggacagca cggcgcccgg
      661 ggaggaacac gcagggcgtc ctccgcgggc gcacgccctg caggtaagag tgaaaaactg
      721 cctgttggtc ccggctcccg cctgaccttg gaagaatcgg cacgtctctt cggcgggatg
      781 attggcaata ggcgcacggc ccttatcttt gcgccaaatt cctattgtgg atactgaaaa
      841 gaaaacattc gatgtgatcg tggtcggcgg cggttgtgta ggacttgctg ccgcatacaa
      901 gatcaatctc cgccatccgc atctcaaagt agctgtactc gaaaaagaag accacgtttc
      961 ccctcaccag acaggacaca attccggcgt cattcattcg ggaatttatt acaagccggg
     1021 ttcgtacaaa gcgaagaact gcgtggaagg acgaagagag atcgtggcgt ttgcgaaaga
     1081 gcataaaatt gcgcacgata tctgcgggaa aataatcgtg gccacgcacg aaagcgagct
     1141 cgcgcacatg aacaaagtgt acaacaacgg cgtggcgaac ggcgtggaag acatcgagct
     1201 gattgacgcc aaaaaaataa aggagatcga accgtacgcc gaaggcattg ccggaatatg
     1261 ggtgggctgc acgggcatca tcgatttccc cggcgtgacg aaaaagctgg cggagctgct
     1321 ggagcagaaa ttcccgggca gcaaagtatt tacttccaca gaagcaaaag atttcgttca
     1381 caaaaacgaa tcgaccgaca tcatcaccaa ccgcggaacg ttttccgcgc gctggctcgt
     1441 gacgtgcgcg ggactgcaga gcgaccgcat tgcgaaaaaa gaaggcgcga aaccggaagc
     1501 ggcgatcgtg ggtttccgcg gcgattatta cgacctgacg gaaaaaggga tgaagaaagt
     1561 gcgcaacctg atctatcctg ttccgaaccc gaaatatcct ttcctcggcg tgcatttcac
     1621 acgcatggtg cacggcggcg tggaatgcgg accgaacgcc gtgttcgttt tcaagcgcga
     1681 gggctacgga aaaacttcgt tcagcctgcg cgacaccgca caggcgctgg gattcggcgg
     1741 cacgtggaaa ttcttcgcga agaacatgaa gttcggctgg gacgaatacc gcggcgcgtt
     1801 ctcgaaacgt tttttcctga aacgcctgcg cacactcatt ccttcgctgg aagcggacga
     1861 catcgtttcc tcgcgcagcg gcgtacgtgc gatggcgctt tcacccgaag gaaaaatgat
     1921 cgacgatttc agcatcgagt tccacggcaa cgcgatccac gtgctgaacg caccttcacc
     1981 ggcggcaaca gcctgtctcg ccatcgggaa agccattgaa gaaatggcga cggagaaatt
     2041 cgggctctga caggaaattt tgaattccag gtattgaatt tcgatttcgg gcattttacc
     2101 cttcgcattt ccattaccac ggaaaacaca gctacccggc agtagccgcg cgaaaaattt
     2161 ttcccgtact tttcatgaac ccgcttgctc agcggtaata tgtgtagacg atatcggttc
     2221 gggagagcag cttccctttt ccgtcaaaaa aattgtacga ggtcagtttc ccgttcccat
     2281 cataaacata cccgacgcgg ttgcagctga ccttcatgtc gtggaggtaa acatcctccc
     2341 cggtcaatcg cccgtcttca taaaaataga catgcatatc aatgggcttg tcgctttcca
     2401 ggtaataata gcttacatcc ttcacgagac gtcccgcgct gtcgctggtg taaatgaacc
     2461 tgtcctgcag cttcttcttt tttaagtaat aatcttttgt ctggacaagc cccgaagaat
     2521 cgtaagtgaa tacggcaaag ccggtaattt ttcccgactg catagacgaa tctctccgga
     2581 gaacaagccg gcctgttgta tcgtaaatat aattctcgaa gcaggaattc tccggcatgc
     2641 catagctgta aaagtcaaca cgcgagatct ttccgcccgg gccgtagtga taaatttcag
     2701 aagagatagc cgtacttttc atcggatcaa aacgctgcac tttcacgatc cgtccggagg
     2761 tatcgaaatc aatcgtgttt tttgtccacg gaggtaattg accaccggcc ggcgaaaacg
     2821 tgtactgcac ttcggatacc tcctgcacat gaagatcatg catcagtccc ggcggatatt
     2881 ttactgcgcc atcctgcgcc gcgcaatcaa gaaaagcggg cacgcaaaat gcgaaaacag
     2941 aaatgaagcg gcggaaaaaa atcatacggc attttttatt gtaacgcaga atcatgcgca
     3001 ggatacacgt tacttattcc gctccccgga tggaaaaaaa cgacatcatc acccagcaca
     3061 acgcgatcgc cgagaaattg gtccaggaaa aatacacgta ttcctcatgg ttcactttcg
     3121 ttcccgactg caacaggagg acaacgaaga ccgcgcaggt gatgctgcac gcaagcacaa
     3181 gcgcctgcac cggcctggct tttcccttct tcttctgtaa ggcta
//
LOCUS       DASTSF010000443         8359 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1194723,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000443 DASTSF010000000
VERSION     DASTSF010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 8359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8359
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1194723"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..455)
                     /locus_tag="VFU15_12875"
     CDS             complement(<1..455)
                     /locus_tag="VFU15_12875"
                     /inference="COORDINATES: protein motif:HMM:NF018449.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HEU4718727.1"
                     /translation="MNREMFTSLKQFRITRPALLMKCAFILIVLFCSLRIYAQSDWPW
                     ARTAVTNGNGSTMSLSVSTDSSGCTFITGWFSSPTITFGSVTLTHTSNGLYDFFIVKY
                     DPNGNVLWARKIGGINYEVGYSVCTDKDGNALVTGYYSDTTLTVGSFTLV"
     gene            873..1217
                     /locus_tag="VFU15_12880"
     CDS             873..1217
                     /locus_tag="VFU15_12880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010294424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HEU4718728.1"
                     /translation="MSDSPKCPRCNYEYTYFNGTVMVCTDCLHEWDPGAKVEAEETAT
                     TKIVDANGNELHDGDTVVVIKDLPVKGASKAVKAGTKVKNIRLVDGDHNISCKIDGFG
                     AMGLKSEFVKKA"
     gene            1436..1906
                     /locus_tag="VFU15_12885"
     CDS             1436..1906
                     /locus_tag="VFU15_12885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718729.1"
                     /translation="MNETENNPAGPLENYTLDDKTYSADLALMDRYTKFSSELLRISL
                     LGFSAIGVLVKYGGGSLQKSGSYAHHQLVFASAFLGLCAAFSLAHRYFATDSMAEFIS
                     ALRKEKSGRNATKEKDRVYFKMKFSGQLILFASVSLVLGIICTFLVFAHSFAQN"
     gene            complement(1942..2478)
                     /locus_tag="VFU15_12890"
     CDS             complement(1942..2478)
                     /locus_tag="VFU15_12890"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEU4718730.1"
                     /translation="MKKRAFPLSKVYGLLEPGPVVLLTTSHHGKDNVMTMSWHTMLDF
                     EPPLIGCVVSNRDHTFSLLKASKECVIAIPAAKLVKKVVQIGNTSGRDTDKFALTGLT
                     KMKASVVHAPLIAECYANIECKVADTSMVNRYCFFVLEAVKAWTDPSEKDPRTLHHLG
                     NGEFRIAGKKIRLPSKMK"
     gene            complement(2574..4640)
                     /locus_tag="VFU15_12895"
     CDS             complement(2574..4640)
                     /locus_tag="VFU15_12895"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HEU4718731.1"
                     /translation="MTILHFIIGWLVVMAGYLLYWKFREDRKAGRSFFNFGDFNVLVA
                     SVTFFIAVYVAMYAFRPEPSFEDPDRQIAFGERTGQAYLVSSAIAEKIKHDPFNTDLH
                     FRMLDEYFSAGNPPDEKAWEREGIRIFSYYQGIVDTDTSARMNDIANLMLAEWYVERQ
                     DRDAGSAAFHLRRVENKSQKYFNFISGKVVMYTAGAESAIPYFLDEIKSNGYVPGAQQ
                     MLAVCYDAVGSEKELRALVYDPTGHARVPHEIKTRIYFLDHKYLRFYEEKFSAMFSGI
                     PFWGFLGDILILLAWFFFLRRLSFASVMKWPQLLFVVCVGVVLTIFSWLLYGFYHYRL
                     HFEPNGKPGNDLLYCFFGIGFIEELIKLIPFLLILRFTRIIQKPIDYILVAAAAGLGF
                     AFFENLLYISQYGLEVILSRALTASVSHMASSAVVAYGFILARYRYEKGWWNIPLFFL
                     FAVLAHGFYDFWLLSGTMRSFAVITLFFYLSEILVFVSFINNALNQSVEEKSAAFNTS
                     RLAALIAGSLILVFTIEYIGSCLTYGTTEGNRTLMNTFVSGGYLVFFLSIRLSNILVI
                     PGKWMKIEFFTGLFPSEILGMRRRDEGFLQRRELVLFPTENSGPADEVLPLHGTMVRE
                     LKSGRETLYEFRAFSTDVFFAVKLKNETTDDGKFRYAELFTKEETAPGEEKPRLAYLG
                     EVMIER"
     gene            4902..5447
                     /gene="msrA"
                     /locus_tag="VFU15_12900"
     CDS             4902..5447
                     /gene="msrA"
                     /locus_tag="VFU15_12900"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEU4718732.1"
                     /translation="MTTPVYDTATFGAGCFWCVEAQFELLDGVVSVVSGFSGGTVKNP
                     SYREVCTGTTGHAEVCQIVYDTTKISYDELLSAFWQCHDPTQLNRQGNDVGTQYRSVI
                     FYHNETQRKKAEYYKQQMNDKNTWGKPVVTEISPFTVFYKAEDYHQDYYDQNGNEPYC
                     QYVIKPKVEKFQEVFRDKLKK"
     gene            complement(5628..6296)
                     /locus_tag="VFU15_12905"
     CDS             complement(5628..6296)
                     /locus_tag="VFU15_12905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718733.1"
                     /translation="MAERLSFSLIFLSLVFFPGTAEAHHAPHFSRGTDFAPQHAPAFF
                     SSDASLPFFISAGADLLNFYDFGKTDDGELLWTGGYLPPGINLEVRKNIYESGNDKSY
                     SVALSPVAGLCFINPGSDLIAVANVPFFLQYNSRLGSTKRSAENKGISFSFGPAVNFM
                     ENLSEYPFGRKFISLDAQFLHRVTREDGGVRFWYARTGMEPGMENLSAKQFHINLGFG
                     RFFG"
     gene            complement(6375..7433)
                     /locus_tag="VFU15_12910"
     CDS             complement(6375..7433)
                     /locus_tag="VFU15_12910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718734.1"
                     /translation="MRLFLAFSLVISALSSYSQTFSGPESVEYDAQQQRWLVGNHSAQ
                     TVSAFYPASGTVVPFASGMTYGPHGLEILGSTLYCCDGSFIRGYDLATGANVFNLDLG
                     ATFLNGLTSDGSDFLFATDFSAKKIYRICPANNSFNLMATTVKTPNGILYDGAGNRCV
                     FVTWGSNAPVQAMSLADSTISTLTTTTLSNCDGITRDHSGNWYVSAWGTNSLNMFDPA
                     FSTAPAAVMTSLNGPADLGINTAGDSIAIPNSNATSVVFYVLNSSGIVAPSSENQLII
                     YADLRDQAISVSSAAPVRATAFLYSSGGTLVAAQRLDNGKTSFSGMTPGLYLVAVTDE
                     NGKLIDRRKIIYGRKPVE"
     gene            7511..8053
                     /locus_tag="VFU15_12915"
     CDS             7511..8053
                     /locus_tag="VFU15_12915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718735.1"
                     /translation="MDINPFQPFSPAKRSPLALIKGICFPLSLVFYLLALMSHAYSDG
                     TYGITCAAMGWSIILTGNLLAFIAWFSNIPFWTAFFMFVISRKKNVHMTAFWLSAVAL
                     LFSMGAFAVHEVMENEGGMSHMVEIAGGAYTWMASYAILLTGTILSTTTLKKVENEKK
                     EKLLREKEAAPANPWDEKRR"
     gene            complement(8067..>8359)
                     /locus_tag="VFU15_12920"
     CDS             complement(8067..>8359)
                     /locus_tag="VFU15_12920"
                     /inference="COORDINATES: protein motif:HMM:NF025838.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4431 domain-containing protein"
                     /protein_id="HEU4718736.1"
                     /translation="TGFGPAPGGDPKSDSREAYYVIWPSSKIDVIATPEQEKTGTVSE
                     HNVDRVQLAGDKAMITPLLNKKVKITGTLSHASTPHQFTKVILNVKTIEAEN"
BASE COUNT         2097 a         2089 c         2276 g         1897 t
ORIGIN      
        1 acaagggtaa atgaaccgac tgtaagtgtt gtatccgagt aatatcctgt caccagtgca
       61 ttcccatctt tgtcggtgca aacagaatat ccaacctcat aattgatacc gccgattttc
      121 ctggcccaaa gcacatttcc atttgggtca tatttgacaa tgaaaaaatc gtataatcca
      181 ttactggtat gagtcaaagt aacagatccg aatgttatgg ttggagaaga aaaccacccg
      241 gtgataaatg tgcacccgga gctatccgta ctcacagaga gactcatcgt gctaccattg
      301 ccattcgtta cagcagtcct cgcccacggc caatcggatt gagcataaat ccggagggaa
      361 caaaaaagga ctatcagaat aaacgcgcac ttcatcaaca gcgccgggcg ggtgatgcga
      421 aattgtttta aagaagtgaa catttctcta ttcattattt aacgaatgat atataaaact
      481 accatttata tatcgctttt tatttaaata cccttttcag aaacgcccga aggcaacgat
      541 tgcaccaact gcacgttttt cacttccccg tttctttgtt gaaaaacctg gccagctgtt
      601 attttactta ctacatagac tgaaacaaga ttgcatttca caaaacattc acgacatgaa
      661 atgcaatctt tccggggcac attaccttcg accccttgaa tgttcctgcc ggagccgcat
      721 gcgcaaagca aacaggtgtg ccatgcagac gtaaggaaaa agaagtgcca ccggctggtg
      781 cagatcgtac gctaaaccca atgctggtct gcaaatcacg tcgaacgcat tggccgctgt
      841 atctttgccg ggtaaaataa acttctacag caatgtccga ctctccgaaa tgcccgcgat
      901 gcaattacga atacacatat ttcaatggga ctgtaatggt ctgcacagac tgtttgcatg
      961 aatgggatcc tggtgcaaaa gttgaagcgg aagaaacggc aactactaaa attgttgatg
     1021 cgaacgggaa tgagctgcac gacggggaca cggttgtggt cataaaagat ctccccgtga
     1081 aaggcgcctc aaaagcggtg aaggcgggaa ccaaggtgaa gaatatccgc ctggtggatg
     1141 gcgatcacaa catcagctgc aagatcgatg gctttggcgc aatggggttg aaatcggaat
     1201 tcgtgaagaa ggcctgatgc cggtctcgtc cgcaaatcgg ttgaacgggt aaaccaagtt
     1261 tagttttctg aagttaatgc cagtggggcg gtccggattt ttcaccggaa gaactggtgt
     1321 ggaccggccg cagcgttttg tatgcggtgt gattttttct tcctgctttg caaaccggtc
     1381 ggcacaacta ttctttctat cttttcagta aacgttataa cccggtcgcg ctcccatgaa
     1441 cgaaactgaa aacaatcccg caggtccgct ggaaaactac acccttgacg ataaaaccta
     1501 cagcgccgat cttgcgctga tggatcgcta tactaaattt tcatcggagc tgctgcgcat
     1561 ttccctgctg ggattttcag cgatcggcgt cctggtaaaa tacggcggcg gttcgctgca
     1621 gaagtccggc agctacgcgc accaccagct tgttttcgcc agcgcttttc tcggtctgtg
     1681 cgctgcgttt tcgctggcgc atcgttattt cgcgacggat tccatggcgg aattcatctc
     1741 ggcactgagg aaagaaaaaa gcggcaggaa cgccacaaag gaaaaagaca gggtttattt
     1801 caagatgaaa ttttccgggc agctgatcct gtttgcttcc gtctcgctgg tgctgggaat
     1861 tatctgcacg tttctcgtct ttgcccattc gttcgcgcag aattaaaaaa caactttgtt
     1921 gccgtcgacg gaaaatttct tctatttcat tttcgacggc agccggatct ttttccccgc
     1981 tatcctgaat tccccgttgc ccaggtgatg cagcgtgcgc ggatcttttt ccgacggatc
     2041 ggtccacgct ttcacggcct cgagcacgaa aaaacagtat ctgttcacca tcgatgtgtc
     2101 ggcgactttg cattctatgt ttgcataaca ttccgcgatg agcggcgcgt gcacaacgga
     2161 agccttcatt ttcgtcagac ctgtgagcgc gaacttgtcc gtgtcgcggc cggatgtgtt
     2221 gccgatctgc acgacctttt tcaccagctt cgcggcggga atggcgatca cgcattcctt
     2281 cgaagctttc agcaggctga acgtgtgatc gcggttgctg accacgcagc cgatgagcgg
     2341 tggttcgaaa tcgagcatcg tgtgccacga catcgtcatc acattgtcct tcccgtggtg
     2401 cgaggtggtg agcaggacga caggcccggg ctcgagcagg ccgtatactt ttgaaagcgg
     2461 gaacgctctt tttttcattg ccgtttctgt tggaaaaaca agttacgggt ttcgcacgac
     2521 ggaaaaacca tcccgcccgt ggcagtacat gtggtttgtg cgcctgtgaa aattcaccgc
     2581 tcgatcatca cctcgccgag ataagccagc cgcggctttt cttcaccggg tgcggtttct
     2641 tcttttgtaa aaagttctgc atagcggaat ttcccgtcgt ccgtggtctc atttttcagc
     2701 tttacagcga aaaaaacatc cgtgctgaat gcacggaatt cgtagagtgt ttcgcgcccg
     2761 cttttcaatt cgcgcaccat cgttccgtgc aggggaagga cctcgtccgc cggccccgaa
     2821 ttttcagtag ggaaaagcac gagctcgcgg cgttgcagga aaccttcatc gcggcggcgc
     2881 atgccgagga tttccgaagg aaaaagtcct gtgaagaact cgatcttcat ccactttccg
     2941 ggaatgacga gaatgttcga caggcggatg gaaagaaaaa aaacaaggta gccgcccgag
     3001 acaaacgtat tcataagcgt gcggtttcct tccgttgtgc cgtacgtgag gcaggagccg
     3061 atgtattcga tcgtgaaaac aagaatgagt gatccggcaa tgagcgcggc gagacgcgag
     3121 gtgttgaatg cggcgctttt ttcttccacc gactggttga gcgcgttgtt gatgaacgaa
     3181 acgaaaacaa gaatttccga caggtagaag aacagcgtga tcacggcgaa ggaacgcatc
     3241 gtgccgctga gcagccagaa atcatagaag ccatgcgcga gaacggcgaa gagaaagaac
     3301 aacgggatgt tccaccagcc tttttcataa cggtaacgcg caagaataaa accataagcg
     3361 acgaccgcgc tcgaggccat gtgcgaaacg gacgcggtga gcgcgcgcga aaggatcact
     3421 tccagcccgt actgcgagat gtaaagcagg ttctcgaaaa aagcgaagcc gagtcctgcc
     3481 gcggctgcaa cgagaatata gtcgatgggt ttctgaataa tgcgtgtgaa gcggagaata
     3541 agcaggaacg ggatgagctt gatgagctct tcgatgaagc cgatcccgaa aaagcagtag
     3601 agcaggtcgt ttcccggttt cccgttgggc tcgaaatgca ggcggtagtg ataaaacccg
     3661 tacagcagcc acgaaaagat cgtcaggaca acgccgacgc agaccacgaa aagaagctgc
     3721 ggccatttca tgacggaagc aaagctcagg cgccggagga aaaagaacca cgcgagcagg
     3781 atgaggatat cgccgagaaa tccccagaac gggatgccgg agaacatcgc tgaaaatttc
     3841 tcctcgtaaa agcgcaggta cttgtgatcg agaaaataga tgcgtgtttt tatttcgtgc
     3901 ggaacacggg catgcccggt gggatcatac acgagcgcgc gcagctcttt ttccgaaccg
     3961 acagcgtcat agcagacagc gagcatctgc tgcgcccccg ggacgtagcc gttgcttttt
     4021 atctcgtcga ggaaataggg gatcgccgat tctgcacctg cggtgtacat taccactttc
     4081 ccggaaatga aattgaagta tttctgcgat ttgttctcca cgcgccgcag gtggaacgct
     4141 gcgcttcccg cgtcgcggtc ctgccgctcg acataccatt ccgcgagcat gaggttcgcg
     4201 atgtcgttca tgcgcgccga agtgtccgta tcgacgatgc cctggtaata cgagaagatc
     4261 cgtatgcctt cgcgctccca ggctttttca tccggcgggt tgccggcaga aaaatattca
     4321 tcgagcatgc ggaaatggag atcggtgttg aacggatcgt gttttatttt ttccgcgatg
     4381 gctgatgaaa cgaggtacgc ctggccggta cgttcgccga atgcgatctg ccggtcggga
     4441 tcttcgaatg aaggttccgg ccggaatgcg tacatcgcca cgtaaacggc gatgaagaaa
     4501 gtgacggagg cgaccaggac gttgaagtcg ccgaaattga aaaagctcct gccggctttc
     4561 cggtcctcgc ggaatttcca gtaaagcagg tagccggcca ttaccacgag ccacccgatg
     4621 atgaaatgca ggatcgtcaa cggagagtat ttatacaaag aaactcaacg cagggcgtaa
     4681 cggcaaacgg cacccgtttc cttgattccg taacttcgtt ctgcgtaact gttaaaaagt
     4741 gttgacgcaa aatccgcccg caaccgtttc ccgtaactag aggcatgaaa cagaccagac
     4801 tttttctact cggtgttctc ctcggcggaa cattttcagc ctgcggccag gccgattcgg
     4861 taaaaaccgc gaagtcaaac aacactacac ccaaaaatgc tatgaccact cccgtgtatg
     4921 acacagccac gttcggcgcc ggatgtttct ggtgcgtcga ggcgcagttc gaattgctcg
     4981 acggcgtcgt ttccgtcgtt tccggtttca gtggcggaac cgtgaagaat ccttcttacc
     5041 gcgaagtgtg caccggcact accggtcacg cggaagtgtg ccagatcgtg tacgacacga
     5101 cgaagatcag ctacgatgaa ctgctctccg ctttctggca gtgccacgac cccacgcagc
     5161 tcaaccgcca gggaaatgat gtcggcacgc agtaccgttc cgttatcttt taccacaacg
     5221 aaacgcagcg caaaaaagcg gaatattaca agcagcagat gaacgacaag aacacgtggg
     5281 gaaaacccgt ggtgaccgag atcagcccgt tcaccgtgtt ctataaagcg gaagattacc
     5341 accaggatta ttacgaccag aacgggaacg agccgtactg ccagtacgtg atcaaaccga
     5401 aagtggagaa gttccaggaa gtgttcaggg acaagcttaa aaagtaaggt taatggttgg
     5461 tgtgttgtga tgtgtggcaa tgccgggaaa atctctcccg gcatttttaa tagatgcacc
     5521 ctgaaatgaa ttattcgtcc ggcaataggc ggaaacaaga gcgaagagcg cgatcgccgg
     5581 ttgcggggaa aaatccgttc gggcgcaatg tatttctgag gcgtgttcta cccgaaaaac
     5641 cgcccgaacc cgaggttgat gtgaaactgc ttcgcggaaa gattttccat gccgggctcc
     5701 atgccggttc ttgcatacca gaagcgcaca cctccgtctt ccctggtgac gcgatgaagg
     5761 aactgtgcat ccagcgaaat aaatttccgt ccgaacgggt attcgctcag gttttccatg
     5821 aaattcacgg cgggaccgaa tgaaaacgaa atgcccttgt tctcagccga acgtttcgtc
     5881 gagccgagcc gggaattgta ttgcaggaag aaagggacat tcgccacggc gataagatcg
     5941 ctcccgggat tgatgaagca gagacccgcc acaggggaaa gcgcaacgga ataggatttg
     6001 tcgttaccgc tttcgtaaat atttttacgc acctcgaggt tgataccggg cggaagatag
     6061 cctccggtcc acagcagttc gccgtcatcg gtttttccga aatcgtaaaa attaagcagg
     6121 tcagcgccgg ccgagatgaa gaacggcagc gaagcatcag aagagaaaaa agcaggtgcg
     6181 tgctgcgggg caaaatccgt accccgggaa aaatgaggtg cgtggtgtgc ttctgcggtt
     6241 cccggaaaaa aaacgaggga aagaaaaatc agagagaagg ataaacgctc agccatttta
     6301 tccggggcaa aacgatcagg aaagcaagct aaggaacccg cggtgttttt tgcagtggcg
     6361 tttggcaggt ggaactattc cacaggtttg cggccgtaaa tgatttttct ccggtcgatg
     6421 agctttccgt tttcatccgt aacggcaacg aggtacaggc ccggtgtcat cccggaaaaa
     6481 gacgttttcc cgttgtccag ccgctgtgcg gcgacgagcg ttccgccgga agaataaaga
     6541 aatgccgtgg cgcgcaccgg cgctgccgaa gaaacagaga tcgcctggtc gcggagatcc
     6601 gcataaatga tcagctggtt ttccgaagaa ggagcgacga tccctgatga attcagcacg
     6661 tagaaaacaa cggaggttgc gtttgaattc ggtatcgcga tggaatctcc ggccgtgttg
     6721 atgccgagat cggcaggacc attgaggctt gtcatgacag cagcgggagc cgttgaaaac
     6781 gccgggtcaa acatattcag cgaattggtt ccccatgcgg aaacgtacca gttgccggaa
     6841 tgatcacgcg taatgccgtc acagttgctg agcgtcgttg ttgtcagcgt ggagatcgtg
     6901 gaatcggcga ggctcatggc ctgcacgggt gcgttgcttc cccaggtgac aaacacgcag
     6961 cggttgccgg cgccgtcata gagaattccg ttcggggttt tgaccgtcgt ggccatgagg
     7021 ttgaacgaat tgttcgcggg acagatgcgg tagatctttt tcgcggaaaa atcggtggca
     7081 aaaagaaaat cgcttccgtc ggaggtgaga ccgttgagga aagtggcgcc gagatcgagg
     7141 ttgaaaacat tggcccccgt ggcgagatca taaccgcgga taaagcttcc gtcgcagcaa
     7201 tacagcgtgc tgccgaggat ctcgagcccg tgcggcccgt acgtcatgcc tgaagcgaag
     7261 ggaacaacgg tgccgcttgc gggatagaat gccgaaaccg tttgtgcact gtggtttccc
     7321 acaagccagc gctgctgctg tgcgtcgtac tccacgcttt ccggaccgct gaacgtttgc
     7381 gaataggagg aaagagcaga aattaccaat gaaaaagcga ggaagaggcg catgaagatc
     7441 gttttatcaa cgttaatcaa aatacgggtg cagtacaagg accggaaggt atttcttcta
     7501 tttttaaaaa atggacatca accctttcca gccattttcc ccggcgaagc gatctcccct
     7561 ggcgttgatc aagggaatct gtttcccgct ttcgcttgtg ttctacctgc tcgcgctcat
     7621 gagccatgcg tattccgacg gaacatacgg catcacctgc gcggcgatgg gatggtcgat
     7681 catcctgaca ggaaacctgc tcgcgttcat cgcctggttt tccaacattc cgttctggac
     7741 ggcgtttttc atgttcgtca tttcgcggaa gaaaaacgtg cacatgacgg cgttctggct
     7801 ttctgcagtc gcgctcttgt tctcgatggg cgcgttcgcc gtgcatgaag tgatggagaa
     7861 cgaaggcgga atgtcgcaca tggttgaaat tgccggcggt gcgtacacct ggatggcttc
     7921 ctacgctatc cttcttaccg ggacgatcct gagcaccacc acgctgaaga aagtggaaaa
     7981 tgaaaagaag gaaaagctgc tccgcgaaaa agaagcggct ccggcaaatc cgtgggatga
     8041 aaaaagacgg tgaaaagtaa ccgcgcttag ttttccgctt cgatcgtttt cacgttcagg
     8101 atcacctttg tgaactggtg aggcgtggaa gcatgtgaaa gcgttcccgt gatcttcact
     8161 tttttgttga gcagcggcgt gatcatcgcc ttgtcgccgg cgagctgcac gcggtcgacg
     8221 ttgtgctcgg aaacggttcc tgttttttcc tgttcaggcg ttgcgatcac gtcgatcttc
     8281 gaggaaggcc agatcacgta atacgcctcg cggctgtcgc ttttcgggtc gccgcccggg
     8341 gccggaccga agccggtat
//
LOCUS       DASTSF010000444         3735 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_573335,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000444 DASTSF010000000
VERSION     DASTSF010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3735
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_573335"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..417)
                     /locus_tag="VFU15_12925"
     CDS             complement(<1..417)
                     /locus_tag="VFU15_12925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718737.1"
                     /translation="MTVGAKLDAENNGRVTGVHRRIIVVPCIPRVSSISCISCVASIA
                     RVSCISSVTRISRISRISCIPSISCVACISCVASVSCVASVSCVASVSGVSGVSGVSS
                     VSGIARVSCISSVAGVSCISCISCIIIISLVIIHEHI"
     gene            445..957
                     /locus_tag="VFU15_12930"
     CDS             445..957
                     /locus_tag="VFU15_12930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718738.1"
                     /translation="MPNLTVAKTSDGLYHYMSGSYKTFEEAANAKTQLMVQGYGDAFI
                     TAYQKGNRIPLDKAGATYVKSPPKDSTDEGSQNAVDKNQVTYKVQLGVFKNDPPADFM
                     QKINSIQGIDKAVTNAGLTRYTAGNTSDFKSISATRDDMKAKGFSDAFIIAFYKGEQI
                     TIQEALELQK"
     gene            1038..1493
                     /locus_tag="VFU15_12935"
     CDS             1038..1493
                     /locus_tag="VFU15_12935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718739.1"
                     /translation="MKKLVTIFLIFAGVAIAGHVHAQTDTTKSKDKGKNQNQSAQKTN
                     AGNSGSGAGNITIDEGGSTKTKSPKASASKTNNGNDQNSGSKPKNDNNTNADQKTSGG
                     SVNPGAPNIAIDEGSAPRAKKRTSAARTGGASPPSGDSAGKADSVVAPK"
     gene            complement(1519..2844)
                     /locus_tag="VFU15_12940"
     CDS             complement(1519..2844)
                     /locus_tag="VFU15_12940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HEU4718740.1"
                     /translation="MKTILIIGAGRSSSSLIQYLLDHAESGSWKVRVGDVDEKNARAR
                     VNGHRHGEGFRFDIKDAAQRAKEISGADIVISMLPAFMHGEVAKDCVKNSKHLVTASY
                     VSAEMRALDAEAKQKNIILLNECGLDPGIDHMSAMKIIHHVKAAGGQMTAFHSYCGGL
                     VAPESNDNPWGYKFSWNPRNVILAGQGTAQYIEDGKYKYLPYNRLFSQAKTIHVDGWG
                     DFDAYANRDSLAYREVYGLENIPTMLRGTLRMPGFCRAWNAFVQLGWTDDSWKIGGAD
                     KHSYATLLDAFLPAGSGTLKKRMAEFLNSTEEDEVIKKLEWAGLFSDEKIKLKDASPA
                     EILQDLLERKWVLKKGDKDLVVMQHIFEFSQKGKKKKIVSSLVVKGEDEIHTAMARTV
                     GLPAAIATRLILGGKITQRGVCVPVSEAIYEPVLEELAKTGIVFTEKEE"
     gene            complement(2980..3363)
                     /locus_tag="VFU15_12945"
     CDS             complement(2980..3363)
                     /locus_tag="VFU15_12945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718741.1"
                     /translation="MKKVLAAFTVIALIAFSSCRKDELPAPAPAASVDINVEYRITSE
                     SGHVTGSYLAPENGVMVEKQFAIDRTNFSFPFQWKSGQKLMIKASNALSSGKEVKVDI
                     YVNSQLFRSGSANAPGTVAVAEGSY"
     gene            complement(3502..>3735)
                     /locus_tag="VFU15_12950"
     CDS             complement(3502..>3735)
                     /locus_tag="VFU15_12950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718742.1"
                     /translation="AKIMEKKEARDTFNRIGQGIYNEVLVLNPHSKQEAIDAVKAKAE
                     RIQRESISSDLIFSSRRALNLLKLLESERKGLD"
BASE COUNT          999 a         1008 c          908 g          820 t
ORIGIN      
        1 gatgtgttcg tgaataatta ccagggaaat gataataata caggaaatac aggaaataca
       61 ggaaacaccg gcaacactgg aaatacagga cacacgggca ataccggaaa cactggaaac
      121 accggaaaca ccggaaacac cggaaacact ggcaacacag gaaacactgg caacacagga
      181 aacactggca acacaggaaa tacaggcaac acaggaaata ctgggaatac aggaaatacg
      241 ggaaatacgg gaaatacggg taacactgga aatacaggac acacgggcaa tactggcaac
      301 acaggaaata caggaaatac tggacacacg gggaatacag ggaacaacga taatccgccg
      361 atggacccca gtgacacggc cattgttttc cgcatccagc ttggcgccta cagtcacccc
      421 atcaacccgc ggattttccg ggatgtgccg aacctcaccg tggcgaaaac aagcgacggg
      481 ctctatcatt acatgagcgg atcgtacaaa acgttcgaag aagcggccaa tgccaaaacg
      541 cagctgatgg tgcagggata tggcgacgcc ttcattacag cttaccagaa aggcaaccgc
      601 atcccgctcg acaaagctgg cgcaacgtat gtgaaatctc cgccgaaaga cagcacggat
      661 gaaggcagcc agaacgctgt cgacaagaac caggtgactt acaaggtcca gctcggtgtt
      721 ttcaaaaacg atccgccggc cgacttcatg cagaagatca attccatcca gggcatcgac
      781 aaagctgtca cgaacgccgg gctcacacgc tacaccgccg ggaacacgag cgacttcaag
      841 agcatttccg ctacccggga cgacatgaag gcgaaagggt tttcggatgc gttcatcatc
      901 gctttctata aaggtgaaca gatcacgatc caggaagcgc tggaacttca gaaatgacat
      961 caaaatgcga tgagcgttat tgccgggctg accggactgg cattatcttt gcaaagtcac
     1021 ttttaaaatc tcataccatg aaaaaactcg tcactatatt tttgattttt gcgggcgtgg
     1081 caattgccgg tcatgtgcat gcacagacgg acaccacgaa atcgaaagac aaaggaaaaa
     1141 accagaacca gtcggcgcaa aaaacaaacg cagggaatag cggttccggc gctggtaaca
     1201 ttaccatcga cgaaggcggc tcgaccaaaa cgaaatcacc caaggcgagc gcatcgaaaa
     1261 cgaataacgg gaacgaccag aacagcggct ccaaaccgaa aaacgacaac aacacgaacg
     1321 ccgatcagaa aacgagcgga ggttccgtaa acccgggcgc acccaacatc gccatcgatg
     1381 aaggaagcgc gccgcgggcg aaaaaaagaa cgagtgccgc acggaccggg ggagcttctc
     1441 ccccgtccgg tgattcggcc ggcaaagctg attcggtcgt tgctccaaaa taaaatccgg
     1501 cgctgaggcc ggattttttt attcttcttt ttccgtgaaa acgatccccg tctttgccag
     1561 ctcttccagc accggttcgt atatcgcttc cgaaaccggc acgcacactc cccgctgcgt
     1621 gatctttccc ccgaggataa gcctggttgc gatcgccgca ggaagcccga cggttctggc
     1681 catcgccgtg tgaatttcat cttcgccttt tacgacgagc gatgaaacga tcttcttctt
     1741 ttttcctttc tgcgaaaatt cgaaaatatg ctgcatgacg acgaggtcct tgtcgccttt
     1801 tttcagcacc catttccgtt cgagaagatc ctgcagtatc tccgcaggcg aagcatcctt
     1861 cagctttatt ttttcatctg aaaagaggcc cgcccactcg agctttttaa tgacctcatc
     1921 ctcttccgta ctattcagaa actccgccat tctttttttc agcgttccgc ttcccgcggg
     1981 aaggaacgca tcgagcagcg tggcgtacga atgtttgtcc gcgccgccga tcttccagga
     2041 atcgtccgtc cagccgagct gcacgaatgc attccacgcc ctgcagaaac ccggcatgcg
     2101 caaagtgccg cgcagcatcg tgggaatgtt ttccagtccg tacacttcac ggtacgcaag
     2161 cgaatcgcgg ttggcatacg catcgaaatc gccccagccg tctacgtgga tggttttcgc
     2221 ctggctgaac agcctgttgt acggaagata tttgtacttc ccgtcttcaa tgtattgcgc
     2281 agttccctgc ccggcaagaa tgacgttgcg cgggttccag gaaaatttat agccccaggg
     2341 attgtcgttc gactcgggcg cgacaagtcc gccgcaatac gaatgaaaag cggtcatttg
     2401 tccgccggcc gctttcacgt ggtgaatgat cttcatcgcc gacatgtgat cgatgccggg
     2461 atcgagaccg cattcgttca gcaggatgat gttcttctgc ttcgcctccg catcgagcgc
     2521 acgcatttcg gccgaaacgt aggaagcggt tacaagatgt ttactgttct tcacacaatc
     2581 cttcgccact tccccgtgca taaaagccgg gagcatgctg atgacgatat ccgcaccgga
     2641 aatttctttc gcccgctgcg ccgcatcctt tatatcaaaa cgaaaacctt ccccgtgcct
     2701 gtgcccgttc acgcgcgccc gcgcattttt ttcatcgaca tcacccactc ttactttcca
     2761 gctcccgctt tccgcgtgat cgagaaggta ttgaatgagc gaggaagaag agcggccggc
     2821 gccaatgatg agaatggttt tcataagaga tgcgaagtta attacactgc gctaaaaaac
     2881 gatcccgcat tttacattcc cgttctgcga aatggaaatt catgaaatga aaagaggcca
     2941 ttcgtgtgaa acggcctccc tcattttaga aacagtaaat caatagctgc cttcagcaac
     3001 cgcaaccgtt ccgggcgcat ttgcagaacc tgatctgaaa agctggctgt tgacgtagat
     3061 atcgactttc acctccttgc cggaagacaa tgcgttcgag gctttgatca tcagcttctg
     3121 accgcttttc cattggaaag gaaaggaaaa attcgtcctg tcgatcgcga attgtttctc
     3181 caccattacc ccgttttccg gggcgaggta agaaccggtt acatgtcccg attcggaagt
     3241 gatccggtat tcgacattaa tatcgaccga agctgcaggt gcgggagcag gaagttcgtc
     3301 tttccggcag gaagaaaatg cgatcagcgc gatgactgta aatgcagcga gtactttttt
     3361 catgatcctt gtgtgtatgg gtttacctga atgaggccgc aaaagtagtc tttaccacac
     3421 aagaagtcaa gttcgtcgat aaaaaatatc tgtttatcta caatctacta taagacaata
     3481 cattacaaag cgacagtccg ttcaatccag gccttttctt tcggattcga ggagtttcag
     3541 caggttgagc gccctccggg acgagaagat caggtcggaa gaaatgctct cacgctggat
     3601 gcgttccgcc tttgctttca ccgcatcaat cgcttcctgt ttcgaatgcg ggttcagcac
     3661 gagcacttcg ttgtatatgc cctggccgat cctgttgaat gtgtcgcggg cttctttctt
     3721 ctccatgatc ttcgc
//
LOCUS       DASTSF010000445         2123 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1405777,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000445 DASTSF010000000
VERSION     DASTSF010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2123
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1405777"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..580
                     /gene="bioD"
                     /locus_tag="VFU15_12955"
     CDS             <1..580
                     /gene="bioD"
                     /locus_tag="VFU15_12955"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011964418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HEU4718743.1"
                     /translation="VGKTVVSAILVEALKADYWKPVQTGSYFSTDSDKIRKYISNTKS
                     VIHPENFVLKQYMSPHAAAELEGMHIGLDQINLPETQNSIVIEGAGGLMVPLNNTEFI
                     VDLIKKLNADVVLVIQNYLGSINHSLLSVDALRFREIPVKGLVFNGAPHKLSEDIILS
                     YSGLKCLGRIQKESVINQEVVKKYAEQFRENL"
     gene            631..1908
                     /locus_tag="VFU15_12960"
     CDS             631..1908
                     /locus_tag="VFU15_12960"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008511680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HEU4718744.1"
                     /translation="MKTVSVSQRDTRYVWHPFTPQLDMEPPVTLVRGEGALLFDEQGN
                     SYIDAISSWWVNIHGHCNAYITKKISEQLNRLEHAIFSGFTHEPAVRLAERLLEKVPH
                     MHRVFFSDDGSTSVEVALKMTFQFFHNKGEKKTKVVALENGYHGDTFGGMSAGARNVF
                     NRAFENFLFDVVHIPVPVKGMEKASLEKLEAIVSGGEVAAFIFEPLVQGAGGMVMYDA
                     PALDALLAVCRKHNVLTIADEVMTGFFRTGKFLATEYCTQQPDIVCLSKGITGGYFPL
                     GATLCTGEIYNAFLSADKTKTFFHGHSYTANPTICAAANASLDLLEQEEPLGNIRRMV
                     SFMQEQQQVFASHEKVREARCTGTILALEIKTGEATEYLNPLAEKVHVYFPPRGVILR
                     PLGNVVYILPPYCTTREQLEKIANEIKLFLDTL"
     gene            1921..>2123
                     /locus_tag="VFU15_12965"
     CDS             1921..>2123
                     /locus_tag="VFU15_12965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VUT family protein"
                     /protein_id="HEU4718745.1"
                     /translation="MKLTRQNNLLLILSAIFISSAITAELISSKVFLVHLHLGNWNIG
                     TFPAVVGILPWPVVFLATDTINE"
BASE COUNT          515 a          566 c          559 g          483 t
ORIGIN      
        1 tgtcggaaag acggttgtct cggctatcct cgtggaggcg ctcaaagccg attactggaa
       61 acctgttcag accggaagct atttttccac cgactccgat aagatccgga aatacatttc
      121 caacacgaag tcagttattc atcccgagaa tttcgtgctg aagcagtaca tgtcgccgca
      181 tgctgccgca gagctggaag gaatgcacat cgggctcgac cagatcaatt tgccggagac
      241 gcagaactcc atcgtcatcg aaggagcggg cggactgatg gtcccgctga acaacaccga
      301 attcatcgtc gaccttatca aaaagctgaa tgcggacgtg gtgcttgtca tacagaatta
      361 tctcggcagc atcaatcatt cccttctttc cgtcgatgca ttgcggttcc gtgaaattcc
      421 cgtgaaggga cttgttttca acggcgcccc gcataagctt tccgaagaca tcattctttc
      481 ctacagcgga ctgaaatgtc tcgggcgcat ccagaaagaa tcagtgatca accaggaagt
      541 ggtgaaaaaa tacgcggagc agttcaggga aaacctgtga agacgcgggc cgtttttctt
      601 ccttcaattt ttccaatctt taccgtctga atgaagacgg tttccgtttc acagagagat
      661 acccgttacg tgtggcaccc gttcacgccg cagctggaca tggaaccgcc ggtgacgctg
      721 gtgcgtggtg aaggtgcgct gctgttcgac gagcagggaa attcctacat cgacgcgatc
      781 tcttcctggt gggtgaatat tcacgggcac tgcaacgctt acatcacaaa gaaaatttca
      841 gagcagctga accgcctcga gcacgccatc ttttccgggt tcacgcatga gcctgcggtg
      901 cggctggcgg aaagactgct ggaaaaagtt ccgcacatgc accgcgtttt tttcagcgac
      961 gacggatcga cgtccgtgga agtagcgctg aaaatgacgt tccagttttt tcacaacaaa
     1021 ggcgaaaaga aaacgaaagt ggttgcgctg gagaacggct accacggcga tacgttcggc
     1081 gggatgtcgg cgggtgcgag aaatgtcttc aaccgcgcgt tcgaaaattt cctgttcgac
     1141 gttgtgcata tcccggtgcc ggtgaaagga atggaaaaag cttcgcttga aaaactggaa
     1201 gcgatcgttt ccggcggaga agtggcggcg tttattttcg agccgctcgt gcagggagcg
     1261 ggagggatgg tgatgtacga cgctcctgcg ctcgatgcac ttctcgccgt ttgcaggaaa
     1321 cataatgtgc tcacgatcgc cgatgaagtg atgaccgggt ttttccggac cgggaaattc
     1381 ctggcgacgg aatactgtac gcagcagccg gacatcgtct gcctttccaa aggaattacg
     1441 ggcgggtatt tcccgctggg cgccacgctt tgcacgggag aaatttacaa tgcgttcctt
     1501 tccgccgaca aaaccaaaac tttttttcac gggcattcct acaccgcgaa tcccacgatc
     1561 tgcgctgccg ccaatgcaag cctcgacctg ctggagcagg aggaaccgct gggaaatatc
     1621 cggcgcatgg tttctttcat gcaggaacaa cagcaggttt tcgcgtcgca tgagaaagtg
     1681 cgtgaagcaa gatgcaccgg cacaatactc gcgctggaaa taaaaaccgg agaagcgacc
     1741 gaatacctga acccgctcgc agaaaaagtg cacgtgtact ttccgccccg cggcgtcatt
     1801 ctccgcccgc tgggaaatgt ggtgtatatt ctgcccccgt actgcacgac gcgtgagcag
     1861 ctggaaaaaa tcgcaaatga aataaaatta ttcctcgaca cactctgatc attctcaacg
     1921 atgaaactca cccgccagaa taatcttctt ctcattcttt ccgcgatctt catctcgagc
     1981 gccataaccg ccgagctgat cagctcgaaa gtttttctcg tgcatctcca tctcgggaac
     2041 tggaacatcg ggacgttccc cgccgtcgtg ggcattcttc cctggccggt ggtctttctc
     2101 gccacggata cgatcaacga att
//
LOCUS       DASTSF010000446         5033 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1269371,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000446 DASTSF010000000
VERSION     DASTSF010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5033
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1269371"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..588)
                     /locus_tag="VFU15_12970"
     CDS             complement(<1..588)
                     /locus_tag="VFU15_12970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010230563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEU4718746.1"
                     /translation="MKKFFFAVFILGIGAWLIAQNGVKPKSPDAVVPEQPKLVVGIVI
                     DQMRFDYVYRFWNKFGNDGFKRLMSQGFNCRNMNFNYVPTYTGPGHTSIYTGTFPAIH
                     GIISNEWYDRESGKDVYCVQDDNVNPVGTTSDEGKRSPVRNLSTTVGDQLRLSDNMKS
                     KVVGVALKDRAAILPAGHTANEAFWYDGSSGNFISS"
     gene            complement(585..953)
                     /locus_tag="VFU15_12975"
     CDS             complement(585..953)
                     /locus_tag="VFU15_12975"
                     /inference="COORDINATES: protein motif:HMM:NF014584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CrcB family protein"
                     /protein_id="HEU4718747.1"
                     /translation="MFQSALLVFLGGGIGSLARLGMTVAVKQFPETHLPVATFLSNSL
                     SCIIMGIALGLFSEKLQDNNLRMFLLVGVCGGFSTFSTFSMETLDLFRRGYVMTGVAN
                     MAFSIGLCITLLAVLTKKQS"
     gene            complement(1020..1595)
                     /locus_tag="VFU15_12980"
     CDS             complement(1020..1595)
                     /locus_tag="VFU15_12980"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEU4718748.1"
                     /translation="MLSWLDTIDHQFFLAVHLWRNSFFDLQMPLVSNRWLWIPLYALL
                     VFLVWKRFRMKSIYIVIAVALLVLFSDQTANLLKNSTKRPRPSYDEALAGQVITPLGR
                     GGNYGFVSGHAANSMAVALFLWLLSGAQKDFPASRRRKWWLLLFPWVILVCWSRVYVG
                     VHFPFDVLGGCAVGALWALVFWFLLRRIIFR"
     gene            1939..2376
                     /locus_tag="VFU15_12985"
     CDS             1939..2376
                     /locus_tag="VFU15_12985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU4718749.1"
                     /translation="MALINPHINFNGNAEEAFNFYRSVFGGEFAKIMRFKDLSGAEFP
                     VPAHEANKIMHIALPIGKNVLMGNDVPESMGRTNENENRSKISISAESREEADRLFNG
                     LSAGGQIEMPISDSPWGSYFGMFRDKYGIEWMVDFDPDYKGQI"
     gene            complement(2743..3393)
                     /locus_tag="VFU15_12990"
     CDS             complement(2743..3393)
                     /locus_tag="VFU15_12990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEU4718750.1"
                     /translation="MEEAKIHEELEKHFPQFEEPLKKLIAENGSLKTYEEGDIVMEKG
                     NYIRSTMLVTEGNIGLYRDGDDGNEFLVYDIGPGEACALSLICALKQERSELKARATE
                     RTSVILVPLKLMDELMLQYRSWYYFVLETYRNRFEKLLEVIDQVIFKGMDERLVFYLK
                     QKTEKSGTTKLHITHQEIANDLNSSREVISRLMKKMEMKGMVKSGRNSVEVVRLDA"
     gene            complement(3420..4301)
                     /locus_tag="VFU15_12995"
     CDS             complement(3420..4301)
                     /locus_tag="VFU15_12995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007658144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydrofolate dehydrogenase/cyclohydrolase
                     catalytic domain-containing protein"
                     /protein_id="HEU4718751.1"
                     /translation="MNLIDGKKIAETIQSEIAEEVKKMAAAGKRAPHLAAVLVGSDGG
                     SLTYVNAKVKACERIGFGSTLIHLAAETTEAELLATVQRLNEDPAIDGFIVQLPLPKH
                     IHEQKIIEAVEPRKDVDGFHPVNVGRMALGLPCYLPATPYGILQLLERCGIETAGKKA
                     VIVGRSHIVGSPMSILLARNSYPGNCTVTLCHSRTPDIAAFTREADIVVVAIGKPHWL
                     KADMIKEGAVVIDVGTTRVDAPGTKLGWRLHGDVKFDEVSPKCSWITPVPGGVGPMTI
                     TGLLQNTLLAAKKDIWS"
     gene            complement(4391..>5033)
                     /locus_tag="VFU15_13000"
     CDS             complement(4391..>5033)
                     /locus_tag="VFU15_13000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014682146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEU4718752.1"
                     /translation="AKAFNDRLDFDGVILTKLDGDTRGGAALSIRSVVNKPIKFVGMG
                     EKMDALDVFYPERMADRILGMGDVVTLVERAQEQFDEKEAKRLQERIAKNKFSFNDFL
                     GQLQQIKKMGNVKDLMGMLPGVGKAIKDIDIDDNAFRHIEAIIYSMTPDEREHPEIIN
                     GNRKNRIAKGSGRTVQEVNRLMKQFEDTRKMMRVMQDKGQMQKMMRNMPRMRR"
BASE COUNT         1175 a         1397 c         1269 g         1192 t
ORIGIN      
        1 gctggaaata aaattcccgc tcgatccgtc gtaccagaac gcttcattcg cggtatgccc
       61 ggcgggaaga atcgcggcac ggtctttcag cgccacgccc accactttcg atttcatgtt
      121 atcggacaaa cgcagctggt cgcccacggt ggtggacagg ttccggaccg gcgagcgttt
      181 tccttcatcg gatgtcgttc cgacgggatt cacgttgtcg tcctgcacgc agtaaacgtc
      241 tttcccgctt tcacggtcgt accattcatt ggaaataatc ccgtggatgg cgggaaaagt
      301 tcctgtgtag atcgacgtgt gacccgggcc ggtatacgtg ggaacgtaat tgaaattcat
      361 gttgcggcag ttgaatccct gcgacatcag ccgtttgaac ccgtcgttcc cgaatttatt
      421 ccagaacctg tacacataat cgaaacgcat ctggtcgatc acgatgccga ccacgagctt
      481 cggttgttcc gggacaacag cgtcgggtga tttcggcttc acaccattct gtgcgatgag
      541 ccatgcgccg atgccgagaa tgaaaacggc aaagaagaat tttttcatga ttgttttttg
      601 gtaagaacag cgagcaacgt aatgcagaga ccgatgctga acgccatgtt ggcgacgccg
      661 gtcatcacat atccccggcg gaacaggtcg agcgtttcca tgctgaaggt ggagaacgtg
      721 gaaaatccgc cgcacacgcc gacaagcagg aacatgcgga gattattatc ctggagcttt
      781 tccgaaaaga gcccgagcgc aattcccatt atgatgcagc tgagggaatt cgacaggaag
      841 gtcgccacgg gcaaatgcgt ttcgggaaat tgctttaccg cgacggtcat gccgagccgc
      901 gccaggcttc cgataccgcc accgaggaaa acaagcaaag ctgattgaaa catgggcaaa
      961 tatactataa gggatgagtg tggagaatgc ggcgcgaaga acgtggctgc aggaaatgct
     1021 taacggaaaa tgatgcgcct gagcaggaac cagaacacga gcgcccacag cgcaccgacc
     1081 gcgcagccgc ccagcacatc gaacgggaaa tgcacaccga cgtatacccg gctccagcag
     1141 acgaggatca cccacgggaa aaggagcagc caccattttc gccgccgcga agccgggaaa
     1201 tctttctgcg cacccgaaag cagccacagg aaaagcgcaa cggccatcga attcgctgcg
     1261 tgacccgaaa caaacccgta gttgccgccg cgtcccagcg gtgtgatgac ctgtcccgcc
     1321 agcgcttcat cgtacgaagg ccgcggccgt ttcgtggaat tcttcagcag gttcgctgtc
     1381 tggtcgctga acaaaacaag cagcgcaacg gcgatgacga tgtaaatgga tttcatgcgg
     1441 aatcttttcc atacgaggaa tacgagcagc gcataaagcg ggatccacag ccagcggttg
     1501 ctcaccagcg gcatctgcag atcgaaaaaa gaattgcgcc agagatgaac agcaagaaaa
     1561 aactgatgat cgatcgtatc cagccagctg agcatgacgg taaaagtact acttccgcga
     1621 tttcagccgg ccccgcaaaa tccgcaggtc ttaacgttca gtcccgtttg ccgccggagt
     1681 tcccggccgg cgctgtacgc gtgagctgct gagcgttccg gcagcaagac caccggcatt
     1741 gacacgcacc cgggcattcg cagcttgcag aagatttatt tcgacctttg atagattgca
     1801 gaaatcgcta catttctaaa aaaatattgc gctatgaacg ggccacgcgt cattgcagcg
     1861 caaccgaaac caaaggcaaa cagaaataac gaacagcgca ggcatacccg taatcaaatc
     1921 atcaaaaaaa taaaaattat ggcactcatc aatccgcaca ttaacttcaa cggaaatgca
     1981 gaagaagcat tcaactttta cagatcagta tttggcggcg agtttgcaaa aatcatgcgt
     2041 ttcaaagacc tgtcaggcgc tgaatttccc gtgccggcac atgaagccaa taaaataatg
     2101 cacattgctt tgccaatcgg caaaaacgtt ttgatgggca atgatgtccc cgaaagcatg
     2161 gggcgaacaa acgaaaatga aaacaggagt aaaatttcaa tcagcgcgga aagcagggaa
     2221 gaagccgaca ggttgtttaa cggactttcc gcaggcggac aaattgaaat gcctatttcc
     2281 gacagcccct ggggttcata ctttggtatg ttcagagaca aatatggtat tgaatggatg
     2341 gtggattttg atccggatta taaaggtcag atttaaccgg aaatgacctg atttgccgaa
     2401 cgcaggacct gcgcctgccg gaaaatccgc aggcatttat cgcggagcag gcttacagat
     2461 tttttgagcc cggttatttt cccgcaaact ttcccagcca tttttttgcc tttttcggcg
     2521 acagcatcta cccgcttaac gaattattgt ttcgacagca aatacgcatc ctatatttgg
     2581 atcacaattc gttgtacaac ggatttcaat acgaccaagt aacattggtt agggtttagg
     2641 gttcttcgtg gcggtcaact ggggcgtagc cgccatgtaa agacaacgat cccggcacag
     2701 ccgggattgt tgattttata aaggttcgta ttcttccgcg cttcacgcat cgaggcgcac
     2761 gacctccacc gaattacggc cgctcttcac cattcctttc atctccattt ttttcatcag
     2821 gcgggagatc acttcccgcg acgaattaag gtcattcgct atttcctggt gcgtgatgtg
     2881 cagcttcgtc gtcccgctct tttccgtttt ctgcttcagg taaaacacga gacgttcgtc
     2941 cattcccttg aaaataacct ggtcgatgac ttccagcagt ttctcgaaac ggttgcggta
     3001 ggtctcgagg acgaaataat accaggaacg gtactgcagc atgagctcgt ccatcagctt
     3061 cagcggaaca agaatgacgg atgttctttc cgttgccctc gctttgagct cgctcctctc
     3121 ctgcttcagc gcacagatga gcgaaagcgc gcaggcttct cccggcccga tgtcgtacac
     3181 gagaaattca ttgccgtcat caccgtcgcg gtaaagaccg atgttccctt cggtaacgag
     3241 catcgtggaa cggatgtaat tccctttttc cattacgata tcgccctctt cgtacgtttt
     3301 cagggaaccg ttttccgcga tgagtttttt cagcggctcc tcgaactgcg ggaaatgttt
     3361 ttcgagctct tcgtgaattt ttgcttcttc catgcggacc cgttcctttt ttgcaatgat
     3421 cagctccaga tgtctttctt cgccgcaagc agcgtgttct gcagcaatcc cgtgatcgtc
     3481 atcggtccca cgcccccggg aacgggcgtt atccagctgc atttcggtga aacctcgtcg
     3541 aacttcacgt caccgtgcag gcgccagcca agcttcgttc cgggcgcatc cacgcgcgtt
     3601 gttccgacat cgatcaccac cgcgccctct ttgatcatat ccgctttcag ccagtgcggc
     3661 ttcccgatcg cgacaacgac aatatccgct tcgcgcgtga acgccgcgat atccggcgtg
     3721 cggctgtgac acagtgtaac cgtacagttc ccgggatacg aatttcgcgc cagcaaaatg
     3781 ctcatcggcg atccgacaat atggcttcgc cccacgatca ccgctttttt tcccgccgtt
     3841 tctatcccgc agcgttcgag cagctgtagg atcccgtatg gcgtggcggg aagatagcag
     3901 ggcaacccga gcgccatcct tccgacgttc accggatgaa acccgtccac atctttccgc
     3961 ggctccacgg cttcgataat tttctgctcg tgaatatgct tcggcaacgg gagctgcacg
     4021 ataaacccgt cgatcgccgg atcttcattg agcctttgta cggtcgcaag caattccgct
     4081 tccgtcgttt cagcggcgag gtgaatgagc gtggacccga aaccgatgcg ttcgcacgct
     4141 ttcacttttg cattgacgta cgtgaggctt ccgccgtcgc tgcccacgag aaccgccgcg
     4201 aggtgcggcg cacgcttccc ggctgccgcc atttttttta cttcttctgc aatttcagac
     4261 tggatggtct ccgcgatttt ctttccgtcg atcaggttca ttgaaatgat gaatgataaa
     4321 tgatgaatga caacccgctt caaagaagca attcagcacc cgggattcag gatttataat
     4381 tcgcgacgcg ttacctgcgc attctcggca tattccgcat catcttctgc atctggcctt
     4441 tgtcctgcat cacgcgcatc attttccgcg tgtcttcgaa ctgcttcatc aggcggttca
     4501 cttcctgcac ggttcttccg cttccttttg caatgcggtt cttgcggttg ccgttgatga
     4561 tctcgggatg ctcgcgctca tcgggcgtca tggaataaat gatcgcctcg atgtgccgga
     4621 acgcgttgtc gtcgatgtcg atatccttga tggcttttcc cacgccgggc agcatgccca
     4681 tcaggtcctt cacgttgccc atctttttga tctgctgcag ctggccgaga aaatcgttga
     4741 agctgaattt gtttttggca atgcgctcct gcaggcgttt cgcttccttc tcgtcgaact
     4801 gctcctgcgc acgttccacc agcgtgacga cgtcgcccat accgaggata cggtcggcca
     4861 tacgttcggg atagaacacg tcgagcgcat ccatcttttc gcccatgccc acgaacttga
     4921 tcggcttgtt cacgacagaa cggatggaaa gcgcagcacc gccgcgtgta tcaccgtcga
     4981 gcttcgtaag aatgacgccg tcgaaatcga ggcggtcgtt gaaggctttc gcc
//
LOCUS       DASTSF010000447         4437 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_67488,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000447 DASTSF010000000
VERSION     DASTSF010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4437
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67488"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            163..2658
                     /locus_tag="VFU15_13005"
     CDS             163..2658
                     /locus_tag="VFU15_13005"
                     /inference="COORDINATES: protein motif:HMM:NF039936.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEU4718753.1"
                     /translation="MKKLLLAFSGLAMAACVNAQVTTVTYNYTGTIDMFTVPSCVTSI
                     TIEARGAQGGQNTSSTTTSGLGATMIGTFSVTPGDQLKILVGQQPSAGSGNGGGGGTF
                     VTDISNNPLIVAGGGGGSSQGADSPDKNGQVGTTGGTGAGGGGIGGTAGNGGSIGASG
                     FQSGAGGGLLTNGADGWTTSTGGMAFVNGGSGGTANAPANGGFGGGGSGSSYVVGGGG
                     GGYSGGGSGGNSTAGVGGGGGSFNAGTNQNNTGGANSGNGMVIITYTTGPGAGPASIS
                     GPLTVCEGSTVTYTAATVPGATSYTWTVPSGWTIVSGQGTNSISLTVDTNPGTIDVTA
                     SDACGTSPATSLSIGVDMMPSAGTVSADSAICSGSTIGLASAGSAGTVTWYEYDGMNY
                     NSLGSGATFTTSPYTAGTYSFVAVANSGVCADDTSAAAVTIVDSVSVGGTASSSIGPD
                     LCAGSDPLLMSAGATGSIEWMFQFNGQGPFISFGTGNPFDPGPPSNQDTGSYVFYAIA
                     TSGVCAADTSNSFMVNVRPTPVVMLGSDTAQCSGSIVLDAQNAGEMFVWNNGPTTQTD
                     TVSSTGTYMVTVTTMYGCSSSDTIMVTINPNPVVDLGADVQQCGGTVTLDAMNNGSSY
                     VWNDNSTGQTLSAASSGTYFVDVTDANGCMGSDTVNVTINTPPTVTGTASSTSACVDD
                     ADVALTGSPVGGTWSGPSVTGSSFDPSIGAGNYSPVYSFTDGNGCTGTASVSITVNAC
                     TGVAEQKAANGVSLYPNPNNGVFTLDVNADVNQLTIEITDVQGRVVYTSDESGVRSGY
                     AKQISLNDQPDGIYFVRVTADGQLHTEKISVIR"
     gene            2886..4097
                     /locus_tag="VFU15_13010"
     CDS             2886..4097
                     /locus_tag="VFU15_13010"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HEU4718754.1"
                     /translation="MKSVLNSIEEAIADIKAGKVVIVVDDEDRENEGDFITAAGNVTP
                     EIINFMATHGRGLICAPLTDERADQLGLELMVRENSSLHETPFTVSIDLKGHGCTTGI
                     SAHDRAKTIQALIDPKIRPEEFGKPGHIFPLRAKNEGVLRRAGHTEATIDLARLAGFE
                     PVGTLVEIMNEDGTMARLPELLQIAKKYGLKIISIKDLIEYRLRTESNIRREVDVQLP
                     TEWGDFKMVAYSHIPTAQPHLAIFKGEWKKDEPVLVRVHSSCMTGDIFGSCRCDCGPQ
                     LHKAMELIQKEGKGIIVYMNQEGRGIGLLNKLKAYRLQEQGRDTVEANLELGFGMDER
                     DYGVGAQILRDLGVTKIRLMTNNPRKRAGLMGYGLEIVDNVPLEIRSNKHNKLYLETK
                     RDKMGHSLKLD"
BASE COUNT         1069 a         1207 c         1229 g          932 t
ORIGIN      
        1 caaaaaggcg actgaaaaaa acgaaaagcc ttttccacac atgacgcgct ggctgcgctg
       61 aggaagctcg gtgactgacc gcttcattta tggggtaaat gcttgtcccc tgaaatgaat
      121 tctttacttt tagggttcca ggcaatcact aacctttcat tcatgaaaaa acttctactt
      181 gcattcagcg gccttgctat ggccgcatgt gttaacgcac aggttacaac agtaacgtac
      241 aattacacgg gaacgataga catgttcacc gttccgtcct gtgtgacttc gataacgatc
      301 gaagcccgcg gagcgcaggg tgggcaaaat acttcttcaa ccactacgtc cgggctgggt
      361 gccaccatga tcggcacctt ttcggtaacg ccgggcgatc agctcaaaat cctggtcggg
      421 cagcaacctt cagcgggatc cgggaacggc ggcggcggcg gaacattcgt aacggatatt
      481 tcgaacaacc cgctgatcgt ggcgggcggc ggaggcggaa gctcacaggg cgccgactct
      541 cctgacaaga acggccaggt cggcacaacc gggggaaccg gtgcaggcgg cggcggtatt
      601 ggcggaacag ccggaaacgg tggaagcatc ggcgcttcag gtttccagtc gggtgccggc
      661 ggcggacttc tcacgaatgg cgctgacggg tggacgacat ccacgggtgg catggcattc
      721 gttaacggtg gttccggtgg aacggccaac gctcctgcca acggcgggtt cggcggcggc
      781 ggaagcggct cgtcttatgt tgtaggcggc ggcggcggcg gatattccgg cggcggctca
      841 ggcggcaaca gcacggcagg tgtaggcggc ggtggcggaa gcttcaatgc cggcacaaac
      901 cagaacaaca cgggtggcgc gaattccggc aacgggatgg tcattatcac ttacaccacc
      961 ggacccggcg ccggtccggc aagcatcagc ggtcctctta ccgtttgcga aggctctaca
     1021 gtcacttata ccgcagccac agtaccgggt gcaacatcct atacatggac ggttccttcc
     1081 ggatggacca tcgtcagcgg acagggaacg aattcgatca gcctcacggt tgacacgaac
     1141 cccggcacga tcgacgtcac tgcaagcgat gcctgcggaa caagcccggc tacctcgctt
     1201 tccatcggcg ttgacatgat gccttctgca ggaacagtat ccgctgattc tgcgatctgc
     1261 tccggaagca cgatcggtct cgcttcggca ggttctgcag gaactgtgac gtggtatgaa
     1321 tatgacggaa tgaattacaa ttcactggga agcggagcaa catttaccac ttcaccgtat
     1381 acagccggaa cttattcttt cgtggcagtt gccaacagcg gtgtctgcgc ggatgatact
     1441 tctgctgcag cggtaaccat tgtcgacagc gtttcggttg gcgggactgc ttcttccagc
     1501 attggtcccg acctttgcgc aggaagcgat ccgctgttaa tgagcgccgg ggcaaccggt
     1561 tcgatcgaat ggatgttcca gttcaacgga caaggtcctt tcatttcatt cggaacaggc
     1621 aacccgtttg atccgggtcc gccgtccaac caggataccg gctcgtatgt attctatgcc
     1681 attgccacaa gcggcgtgtg tgcagcagat acgtcgaatt cattcatggt caatgtacgc
     1741 ccgacgcccg ttgtcatgct gggctccgat accgcgcaat gcagcggcag catcgtcctc
     1801 gacgcacaaa acgcgggtga aatgttcgta tggaacaatg gtccgacaac gcagactgat
     1861 accgtcagtt ctaccggaac ttacatggtg acggtaacca cgatgtacgg ctgctcttct
     1921 tccgatacga tcatggttac gatcaacccc aacccggttg tcgatctcgg tgccgacgtg
     1981 cagcaatgcg gcggcacagt aacgctcgac gcgatgaaca acggatcgtc ctacgtatgg
     2041 aacgacaaca gcaccgggca gacactttcg gcggcaagca gcggaaccta ctttgtggac
     2101 gtgactgatg cgaacggatg catgggcagc gatacggtca acgtaacgat caatacgccg
     2161 ccgacggtta cgggaaccgc ttcttccact tcagcatgcg tggatgacgc agacgtagcg
     2221 ctgaccggat cgccggtggg cggaacatgg tcgggcccga gcgtaacagg ttcttcattc
     2281 gatccttcga tcggtgccgg caactattct cccgtctatt ccttcactga cgggaacggc
     2341 tgtacgggaa cggcttccgt ttccatcacg gtgaacgcct gcacaggtgt ggcggaacag
     2401 aaagcagcaa acggcgtcag cctttacccg aacccgaaca acggcgtgtt tacgcttgac
     2461 gtaaatgcag atgtcaacca gctcacgatc gagatcaccg atgtgcaggg acgtgtggtg
     2521 tacacttcgg atgaaagcgg cgtccgcagc ggctatgcga aacagatctc gctcaatgat
     2581 cagccggatg gaatctattt cgtacgcgtc actgccgacg ggcagctgca tacggagaaa
     2641 atttccgtga tccgctaaaa gcaaaactga tttttacagg aaagcccggc ccggtaatgg
     2701 ccgggctttt tcatgcagct ttcccggcta aattttcacc gccagctttt gtgtctcccg
     2761 acccgttttt ttgacccaag ggcccggttc attccgtgag cgggccaccg ccctttcttt
     2821 tacaggaact gtaactacat ttcctgctga aaactatacc gtacctttgc agaaaccttt
     2881 tttgcatgaa atctgtgctg aattcaattg aagaagcgat cgccgatatc aaagcgggga
     2941 aagtcgtaat cgtggtggat gatgaggacc gtgagaacga aggcgatttt atcaccgctg
     3001 caggaaacgt cacgcccgag atcatcaatt tcatggctac gcatggccgc ggactcatct
     3061 gcgccccgct caccgacgaa cgcgccgacc agctcggact cgagctcatg gtgcgtgaaa
     3121 attcctcgct ccatgaaacc ccgttcactg tttccatcga cctgaaaggg catggctgca
     3181 caaccggaat ttccgcgcac gaccgggcaa agaccataca ggcgctgatc gatccgaaaa
     3241 taagaccgga agagttcggc aagccggggc atatttttcc gctgcgcgca aagaacgaag
     3301 gcgtgcttcg ccgggcagga cataccgaag cgacgatcga cctcgcgcga cttgcagggt
     3361 tcgaaccggt gggaacgctg gtggagatca tgaacgagga cggaacgatg gcgcgtcttc
     3421 ccgaactgct ccagatcgcg aaaaaatacg ggctgaaaat tatttccatc aaagacctga
     3481 tcgaataccg tctccgcacg gaatcaaata tccggcgtga agtagatgtg cagcttccca
     3541 ccgagtgggg cgatttcaaa atggtcgcgt actcgcacat ccccactgcc cagccgcacc
     3601 tcgccatttt caaaggcgaa tggaaaaaag acgaacctgt tcttgtgcgc gtgcacagct
     3661 cctgcatgac cggcgatatt ttcggttcgt gccgttgtga ctgcggaccg cagctgcaca
     3721 aggcgatgga gctgatccag aaagaaggca aaggcattat cgtttacatg aaccaggaag
     3781 gacgcggcat cggattattg aacaagctca aagcgtatcg cctgcaggaa cagggccgcg
     3841 acacggtgga agccaatctc gagctcggtt tcggaatgga tgaacgcgat tacggcgtcg
     3901 gtgcgcagat cctgcgcgat ctcggcgtca cgaagatccg cctgatgacg aacaacccgc
     3961 gcaagcgtgc cggcctcatg ggctacgggc tcgagatcgt ggataatgtt ccgctggaga
     4021 tccgttcgaa caagcacaac aagctttacc tcgaaacgaa gcgcgacaag atggggcact
     4081 cgctcaagct ggactgaatc attgagaatt cagcattgtt atttagcgga atccaggcta
     4141 tttccatttc aacacaaagg ccacgaagtt ttttcacaaa gttcacaaag aaaaacgacg
     4201 gatgaataac tgagaattca gcgttgttat ttagcggagt tctgtggcgg cttgttaagt
     4261 atttccattt caacacaaag gtcacgaagt ctttttcacg acgttcacaa agaaaaacgc
     4321 cggttgaata actgagaatt cagccatcaa aattattcag cgaaatcctg tggcggcttg
     4381 ctgattattt ccatttcaac acaaaggcca cgaagttttt tcacaaagtt cacaaag
//
LOCUS       DASTSF010000448         2068 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1403574,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000448 DASTSF010000000
VERSION     DASTSF010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2068
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1403574"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..606
                     /locus_tag="VFU15_13015"
     CDS             <1..606
                     /locus_tag="VFU15_13015"
                     /inference="COORDINATES: protein motif:HMM:NF013168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]
                     imidazole-4-carboxamide isomerase"
                     /protein_id="HEU4718755.1"
                     /translation="EEAGLKRLHIVDLDGAKAGKVKNISVLEEIASATSLQIDFGGGI
                     KTTGELKAVLDAGAVYASVGSVAVKAPELFTEWVHAFGPEKFFLGADVRDGKIAVQGW
                     LEQTEIDAVDFIHEQMRKRIAHVFCTDISRDGLMTGPSLTFYRHLLFQCPGLRLVASG
                     GVSSLEDIRALDRLGCDGVIVGKAIYEGKIPLAELSAINEQ"
     gene            651..1412
                     /gene="hisF"
                     /locus_tag="VFU15_13020"
     CDS             651..1412
                     /gene="hisF"
                     /locus_tag="VFU15_13020"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014034420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HEU4718756.1"
                     /translation="MLTKRIIPCLDVKEGRTVKGVNFEGLRDAGDAVELAARYAEEGA
                     DELVFLDITATNEKRKTMAELVKRVAAQINIPFTVGGGISSVEDVGILLDHGADKISV
                     NTSAFNDRGLIDRLAKKFGSQCIVVAIDVKKENGGWFVYLNGGKTATQVKALDWAKEA
                     EESGAGEILLTSMNSDGTKKGFANEITRAVAGTVHIPVIASGGAGTMQHFADVFTEGR
                     ADAALAASIFHFNEIQIATLKKFLAERNIPVRELN"
     gene            1432..2022
                     /gene="hisIE"
                     /locus_tag="VFU15_13025"
     CDS             1432..2022
                     /gene="hisIE"
                     /locus_tag="VFU15_13025"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013871059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HEU4718757.1"
                     /translation="MKPDFSKSGGLIPAIIQDARTNVVLMLGYMNAEAFEKTKKENRV
                     TFFSRSKNRLWTKGGESGNFLDVKNISLDCDNDTLLIKVVPHGPVCHTGADTCWGETN
                     AGNFLAHLEEVIQWRKNADPSSSYTASLFAIGINKIAQKVGEEAVEVVIEAKDNNDEL
                     FLNESADLLFHYLVLLAAKGKTLKDVTDVLEKREKK"
BASE COUNT          537 a          479 c          619 g          433 t
ORIGIN      
        1 gaagaggcag gactgaagcg cctgcacatc gtcgacctcg acggagcgaa agcgggaaaa
       61 gtgaagaaca tttccgtgct ggaggagatc gcgtccgcca cttcgctgca gatcgatttc
      121 ggcggcggca taaaaacgac gggcgaactg aaagccgttc tcgacgcggg cgcggtttat
      181 gcatccgtcg ggagcgtggc ggtgaaagcg ccggaacttt ttacagaatg ggtgcacgcg
      241 ttcgggccgg aaaaattttt tctcggtgcg gacgtgcgtg acggaaaaat tgccgtgcag
      301 ggatggctcg agcagacgga gatcgatgcg gtggatttca ttcatgagca gatgcgcaaa
      361 cggatcgccc acgtgttctg tacggacatt tcccgcgacg gcctgatgac aggtccttcg
      421 ctgacatttt accggcatct cctttttcag tgcccgggac ttcgactcgt tgcgagcggc
      481 ggcgtttcat ctttggaaga catccgtgcg ctcgaccgtc tcggctgcga cggcgtgatc
      541 gtgggaaaag cgatctatga aggaaaaatt cccctggcgg aactttccgc cattaatgaa
      601 caataatgcc cgattcacaa cccgcgattc atcatttata atttccttaa atgctcacga
      661 aacgtatcat tccctgtctt gacgtgaaag agggccgcac cgtgaaaggt gtgaacttcg
      721 aaggattgcg cgatgccggc gatgcggtgg agctggcggc gcgttatgcc gaagaaggcg
      781 cggacgagct ggtttttctc gacatcaccg ccacgaatga aaaacggaaa acgatggcgg
      841 agctcgtgaa gcgagtcgcg gcgcagatca atatcccgtt cacggtgggc ggaggaattt
      901 cttcggtgga agacgtaggg atattgctgg accacggcgc agataaaatt tcagtgaaca
      961 catcagcgtt caatgatcgc ggactcatcg accggctggc gaagaaattc ggaagccagt
     1021 gcattgtcgt ggcgatcgat gtaaagaagg agaatggcgg gtggttcgtt tacctcaatg
     1081 ggggaaaaac agccacgcag gttaaagcac ttgactgggc gaaggaagcg gaagaaagcg
     1141 gggccgggga aatcctgctc acgtcgatga acagcgacgg aacgaaaaaa ggttttgcca
     1201 atgagatcac gagggctgtt gcaggaacgg tgcacattcc agtgatcgct tccggcggtg
     1261 cgggcacgat gcagcatttc gcggatgttt tcacggaagg ccgggcggat gccgcgctgg
     1321 cagcgagcat ttttcatttc aatgaaatac agatagccac gctgaaaaaa tttcttgcgg
     1381 agcggaacat cccggtccgt gagctgaact gaattcacca tggaaaaaga actgaagcct
     1441 gatttctcca aaagcggcgg actcattccc gcgatcatcc aggatgcgcg gacgaatgtt
     1501 gtgctcatgc tcggctacat gaatgcggag gcatttgaaa aaacaaaaaa ggaaaaccgt
     1561 gtcacgttct tcagccgctc gaagaacagg ttgtggacga aaggcgggga gagcgggaat
     1621 tttctcgacg tgaaaaacat ttcgcttgat tgcgacaatg atactttgct gattaaagtt
     1681 gtcccgcacg gacccgtgtg ccatacgggc gcagatacct gctggggtga aacgaatgcg
     1741 ggaaatttcc tcgcgcatct ggaagaagta attcagtggc ggaaaaatgc ggacccttct
     1801 tcctcgtaca ctgcttctct ttttgcaata ggaataaata aaattgcgca gaaagtagga
     1861 gaagaagctg tggaggtcgt gatcgaggcg aaagacaata acgatgaact tttcctcaat
     1921 gaatcggcgg accttctgtt tcattacctc gtgctgctcg cggcaaaggg gaaaacgctg
     1981 aaggacgtga cggacgtgct ggagaaaaga gagaagaaat gaaacacatc gctccgcaga
     2041 tgcttcccgg ctaaattgat cgagaatc
//
LOCUS       DASTSF010000449         3179 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_744062,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000449 DASTSF010000000
VERSION     DASTSF010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3179
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_744062"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(74..1834)
                     /locus_tag="VFU15_13030"
     CDS             complement(74..1834)
                     /locus_tag="VFU15_13030"
                     /inference="COORDINATES: protein motif:HMM:NF026253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HEU4718758.1"
                     /translation="MRTRFFLLLPVIVALLSSCSHEKGKLRAYVSVNGRYGFIDEFGN
                     TCVDMKFEEAWSYMRGSAVVKANGKFGLIDKEGNYIVEPQYDSAISFSSECVIVMKNN
                     LFGFMETGTGKMILPLQFENAVPYSDGLCLVVKGKSVGLVNGEGKIVCPVEMQNIDQM
                     LNVGAKIIKTDTDQVQMLTNLIQGIGELKYGIINSRGEVIIPAIYNDIFDNIPDAYYY
                     YPFVRSPEFAEDTTPKVNGAPPVLIGKYGITDTAGKTIFKPQFDELPVYGDGLFRICV
                     NKKYGFADTSGKIVITPQYDYVTAFSEGKAIVSEKTGNFIINRSGKIIAKNISPDAGA
                     FLFKNGRARCVSGNKLYGFIDSTGHEIIPPQFDVAEDFSHGRSIVSVNGKCGLISPDG
                     NFVVKPMYYFLFDLEGGYYQTKSESGRAGIIDSTGKIIADTVYDEVFHFQKNYFCVEQ
                     NGLNGVLDLDGKTIFPAASSENIFFFSKRARVKVNDKWGIINEEGDYLLPAEYDSLGM
                     LFKGYSTVVMNGRCGTIDSTGRIIIPPKFDELRPIINGFAVFSEKGRYGYVNAKGEIV
                     IDPQYEDAAVFVDPDRKEFE"
     gene            complement(1897..2526)
                     /locus_tag="VFU15_13035"
     CDS             complement(1897..2526)
                     /locus_tag="VFU15_13035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718759.1"
                     /translation="MHLRRLIAFTFFFFSAFVLSANGNDTSYIYVHILHGSKPQRNCP
                     DDSDAYYMLGGKLGGHVVIQLDSFDYGFSYTTKHVHPFARKKKSCCAGVYECDSASCQ
                     LEGWSRDKVTTIKIPVSPAVKKQLRDSMVKWHQHPPFDYAFFGMRCASTAYYLLSIAG
                     VFPKASKAKSMRKAFWPGAFRKKIMHVARKKHYAVTVHPGSRFRRWEGG"
     gene            complement(2566..>3179)
                     /locus_tag="VFU15_13040"
     CDS             complement(2566..>3179)
                     /locus_tag="VFU15_13040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718760.1"
                     /translation="PLTKEILADAEIAQPLIAVSDFSYYAEKTFGHNFVMLGDSGKFL
                     DPIFASGVYLGMNSAKLFADALDVLMKEGKEKGMEAMKEKKKHIDGAYDLVEKFVNIY
                     YDPAAFNLAELSSVSESNYKGYETAFSLVHYLLAGDFFNRYEEYSGFLDMLRDPKQFA
                     RWKNLVVDRPHIHEKSCGADWNLIFGDIDDELVRSTSINREKA"
BASE COUNT          741 a          818 c          807 g          813 t
ORIGIN      
        1 tctaaggtaa ggaaaaagta gtgatcggtc agtattgagc cgtgcgacat caggtaatcc
       61 tgaatcacac ttcttactca aattccttcc ggtcgggatc gacgaacacc gcggcgtctt
      121 cgtattgcgg atcgatgacg atctcgcctt ttgcattgac atagccgtag cggccttttt
      181 cggaaaacac ggcaaagccg ttgatgatag gcctgagctc atcgaacttc gggggaatga
      241 tgatcctgcc cgttgagtcg atcgttccgc acctgccgtt catcacgacg gtagaatagc
      301 ctttgaaaag cataccgaga ctgtcgtatt ccgcgggaag aaggtaatcg ccttcttcat
      361 tgatgatgcc ccatttgtca ttcactttta cgcgggcccg tttgctgaaa aagaaaatat
      421 tttccgaaga ggcggcggga aaaatcgtct tcccgtcaag atcgagcaca ccgttcagcc
      481 cgttctgttc cacgcagaaa taatttttct ggaaatgaaa aacttcgtcg taaaccgtat
      541 ctgcaataat ttttccggtg gaatcgatga tacccgcacg accgctttca cttttcgtct
      601 ggtaatagcc gccttcgaga tcaaagagga aatagtacat cggcttcacc acgaaatttc
      661 catcggggct gatcagtccg catttcccat tcacggaaac gatggagcgg ccgtggctga
      721 aatcttccgc cacatcgaac tgcggaggaa tgatctcgtg tcccgtggaa tcgataaaac
      781 cgtaaagctt gtttcccgaa acgcagcgcg cgcggccgtt tttgaagagg aacgctcccg
      841 cgtcaggact aatgttcttt gcgatgatct tccctgaccg gttgatgatg aaattccctg
      901 tcttttcact gacaatcgct ttcccttcgc tgaacgcagt gacgtaatcg tactgcggcg
      961 tgatcacgat cttcccggat gtatccgcga aaccgtattt tttattgaca cagatgcgga
     1021 acaacccgtc gccgtatacg ggaagctcgt cgaactgcgg tttgaaaatt gttttcccgg
     1081 ccgtatctgt gatcccgtat ttcccgataa gcacgggcgg agcgccgttc actttcggtg
     1141 tcgtgtcttc tgcaaattcc ggcgaacgga cgaacgggta atagtaatac gcatccggga
     1201 tgttgtcgaa aatatcattg tagatcgcgg gaatgatcac ttccccgcgc gagttgatga
     1261 tcccgtattt cagttcgccg attccctgga tgaggttcgt gagcatctgc acctggtcgg
     1321 tatccgtttt tatgatcttc gcgccgacat tgagcatctg gtcgatgttc tgcatttcaa
     1381 ccgggcagac aatttttcct tcgccattga caaggccgac cgattttcct ttcaccacga
     1441 ggcagagccc gtcgctgtac gggaccgcat tttcgaattg cagcggaagg atcattttcc
     1501 cggtgcccgt ttccatgaac ccgaaaaggt tgttcttcat cacgatcacg cattcggagc
     1561 tgaatgaaat agccgaatcg tattgcggct ccacgatgta atttccttcc ttgtcgatca
     1621 gtccgaattt tccgttcgct tttaccacgg cgcttccgcg catgtacgac caggcttctt
     1681 cgaatttcat atcgacgcag gtgttcccga attcatcgat gaagccgtac ctgccgttta
     1741 cgctcacgta cgcccgcagc tttccttttt cgtgcgaaca ggaggaaagc agtgcgacga
     1801 tcaccggcag caggagaaaa aatcttgtgc gcatgctcaa aactaactaa taagtgaaga
     1861 agaaggtcga agaggaaatg ccaaataagc tgaaaatcat ccgccttccc agcgacggaa
     1921 acggctgccc gggtgaaccg ttaccgcgta atgttttttc cgcgccacgt gcatgatctt
     1981 tttcctgaat gcaccgggcc agaatgcttt ccgcatgctt ttcgctttcg acgctttcgg
     2041 gaaaacacct gcaatggaaa gaaggtaata ggcggtcgat gcacagcgca tgccgaagaa
     2101 cgcataatcg aacggcgggt gctggtgcca tttcaccatg ctgtcgcgca gctgtttctt
     2161 tacagcgggc gaaacgggaa tttttatcgt cgtcacttta tcgcgcgacc agccctcgag
     2221 ctggcaggag gcggaatcgc attcgtacac gccggcgcag cagctttttt tctttcgtgc
     2281 aaaaggatgg acgtgctttg tcgtgtagct gaaaccgtaa tcgaaggagt cgagctgtat
     2341 gaccacgtgg cccccgagct ttccgcccag catgtagtac gcgtcgctgt catcgggaca
     2401 attgcgctgc ggtttcgagc cgtggaggat gtgaacgtag atgtacgacg tgtcattgcc
     2461 gttcgcggaa agcacgaacg cgctgaagaa aaagaaagtg aatgcaatga gccgtcgcag
     2521 atgcatattc aaaattaata caggaaataa aaaaagatac tcccgctatg ccttttcccg
     2581 gttgatgctt gtgctgcgca ccagctcgtc gtcgatatcg ccgaaaatga ggttccagtc
     2641 ggcgccgcag cttttctcat gtatatgcgg ccggtcgaca acgagatttt tccatcgcgc
     2701 gaattgtttc gggtcgcgca gcatgtcgag gaaaccgctg tattcttcgt aacggttgaa
     2761 gaaatcgccc gcgagcaggt aatgcacgag cgaaaaggcc gtttcgtatc ctttgtaatt
     2821 ggattcggaa acggagctga gctccgccag gttgaacgcg gccggatcgt aataaatgtt
     2881 gacgaatttt tcgacgagat catacgcgcc gtcgatgtgc tttttctttt ccttcatcgc
     2941 ttccatgcct ttttcttttc cttccttcat cagcacgtcg agcgcatcag cgaataattt
     3001 cgcactgttc atgccgaggt aaacgccgga agcgaagatc gggtcgagga attttcccga
     3061 atcgccgagc atcacgaagt tgtgcccgaa tgttttttcc gcgtaatagg aaaaatcgga
     3121 aacagcaatg agcggctgcg caatctccgc gtcggcgaga atttctttcg tcagcggag
//
LOCUS       DASTSF010000450         2491 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_68154,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000450 DASTSF010000000
VERSION     DASTSF010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2491
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68154"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..841)
                     /locus_tag="VFU15_13045"
     CDS             complement(<1..841)
                     /locus_tag="VFU15_13045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718761.1"
                     /translation="MKSCFFLSASLLVACSVKAQVIFQKTYGGHQYENSDCMYRGPGS
                     ITRTAEGGYAICHATASYGVPASTSGESLYFLKIDDNGDTLVTRAYNSGGNSIGRSIF
                     EAAGGGYVMGATMGGAAGTSLIRVNANGDTLWTRSFSPIPGGFGGTGYYGFPFNDGGF
                     GAVGEYGETVQNHSTFCAGLAKVDANGNLVFGKTYYYNPALFNGTGMRFYSASPTSDG
                     GVLATGYIDDMEIDVVVVRMDSTGNVLWAKSYDLSTEEAGLMAKELPDHSIAVTGYVA
                     LSTT"
     gene            complement(909..1808)
                     /locus_tag="VFU15_13050"
     CDS             complement(909..1808)
                     /locus_tag="VFU15_13050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013455863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEU4718762.1"
                     /translation="MTVKRNALGKGLSALLENANTDITSSGKMKGETTAIVGAVSNIP
                     LAQIEANPFQPRTHFEEQALTELSESIRLHGIIQPVTVRKMGYDRFQLISGERRFRAS
                     QLAGLETVPAYVRVANDQSMLEMALVENIQRENLDSIEIAISYKRLIDECSLTQEELA
                     AKVSKQRSTITNYLRLLKLPAEVQLGIREKKISMGHARALINVEKQDDMVDLYHRITE
                     EDLSVRDVEELARDVKKIEPRIKHKKLQGDLKEKYADKIRSLSKQYNAKISLKSNNLG
                     EGKLVIPFANETDLERIIDALDI"
     gene            complement(2150..>2491)
                     /locus_tag="VFU15_13055"
     CDS             complement(2150..>2491)
                     /locus_tag="VFU15_13055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParA"
                     /protein_id="HEU4718763.1"
                     /translation="LGKLLNTIKIVQTRINTDLQIEGILMTMYDGRLRLSNQVVEEVK
                     THFQSMVFDTIIQRNTKLGEAPSFGETIIMHDATSKGAINYLNLAREILQKNNMTRLS
                     DEEKIINITQG"
BASE COUNT          503 a          644 c          679 g          665 t
ORIGIN      
        1 cggtggtgct gagcgcaaca taacccgtaa cggcgatgct gtggtcaggc aactctttcg
       61 ccatgaggcc ggcttcttcg gtactgagat catagctctt cgcccaaagc acattgcctg
      121 tcgaatccat ccgcacaacg accacatcta tctccatgtc gtcaatatag ccggttgcaa
      181 gaacaccgcc gtcgctggtt ggtgatgcgg aataaaagcg catccccgtt ccgttgaaca
      241 gggcaggatt ataataatac gttttcccga aaacgagatt gccattcgca tccacttttg
      301 ccagccctgc acagaacgtg gaatggtttt gcactgtttc cccgtattcg ccaacagctc
      361 cgaagccgcc gtcgttaaat gggaaaccgt agtaaccggt tccgccaaaa ccaccgggaa
      421 tgggtgaaaa gctgcgcgtc cagagcgtgt caccgttcgc attcacgcgg ataagcgaag
      481 tcccggcagc tccgcccatc gtggccccca tgacgtaacc gccgcctgcc gcttcgaaaa
      541 tgctccgtcc gatgctgttg ccgccgctgt tgtaggccct ggtgaccagc gtgtcgccgt
      601 tgtcatcgat cttcagaaaa tagaggcttt cacccgacgt gctggcggga acgccgtagc
      661 tggccgtggc gtgacagatc gcatagccgc cttccgccgt gcgcgtgatg ctgcccgggc
      721 cgcggtacat acaatctgaa ttttcatatt gatgaccacc gtatgtcttc tggaagatca
      781 cctgcgcttt tacggaacag gcgacgagga gagacgcgga aagaaagaag caggatttca
      841 tgcaatgaaa ctacatcttt ccggagggca acgcaacagg actgtcccgg gtccttacgt
      901 cttcaaaact aaatatccag cgcgtcgatg atgcgttcaa gatcggtttc attcgcaaac
      961 ggaatgacga gcttgccttc accaaggttg ttgcttttga gactgatctt cgcattgtat
     1021 tgcttcgaca ggctcctgat cttgtcggcg tatttttcct tgagatcgcc ctggagtttt
     1081 ttgtgcttga tgcgcggctc gatctttttc acgtcgcggg cgagctcttc cacatcgcgg
     1141 acagaaagat cttcttccgt gatgcggtgg taaaggtcaa ccatgtcgtc ctgcttctcg
     1201 acgttgatga gcgcgcgcgc gtggcccatg gagattttct tttcgcggat gcccagctgc
     1261 acttcagccg gaagtttgag gaggcgcagg taattggtaa tggtggagcg ctgcttgctc
     1321 actttggcag caagctcttc ctgtgtgaga ctgcattcat caatgaggcg tttgtagctg
     1381 atggcgatct cgatggagtc gagattttcg cgctggatat tttccacgag cgccatttca
     1441 agcatggact ggtcgttggc gacgcgaacg taagcgggaa cggtttcgag cccggccagc
     1501 tgcgaagcgc ggaaacggcg ttcacctgag atgagctgga aacggtcgta acccattttg
     1561 cgcacggtga cgggttgtat aatgccgtgg aggcggatgg attccgaaag ctcggtgagc
     1621 gcctgctcct cgaagtgcgt gcggggctgg aacgggttgg cttctatctg cgccagcgga
     1681 atattggaaa cagcgccgac gatggcagtc gtttcccctt tcattttgcc ggaagaagtt
     1741 atgtctgtgt ttgcgttctc cagcaaagcg ctgagtcctt ttccaagtgc gtttcttttg
     1801 acggtcatga tcgttcgatg ttcagttcaa cagttaaaat tgggcgttct ccgtataacc
     1861 tgcaaggtct gaacctgcaa ggttgcctgt ttcgggtgcg attgattgta ttttattgat
     1921 aatcagtgcc tggtttatat ttcccgcctt tcggggggag gacctgcaag gttataacct
     1981 gcaaggttgt attctttcac aattttccag tcttttttat tgataatcaa tgcttaacgt
     2041 gcacttctgg ctttcccggc ggaaactgga ggactttctc tttgtacgcc gaatgacgcc
     2101 cgtttaatcc tcccgcaaaa gcaggatccg gccccggggg cgaactcatt tacccctgcg
     2161 tgatgttgat tattttttcc tcgtcgctca atcgcgtcat gttgtttttc tgcaggatct
     2221 cccgcgcgag gttaaggtaa ttgatggcgc ctttgctggt agcgtcgtgc atgatgatcg
     2281 tttcgccaaa gctcggtgct tcgcccagct tggtgttgcg ctggatgatc gtgtcgaaga
     2341 ccatgctctg gaaatgcgtt ttcacttctt ccaccacctg gttgctgagg cggaggcggc
     2401 catcgtacat ggtcatcagg ataccttcta tctgcagatc ggtattgatg cgcgtttgta
     2461 caatcttgat ggtattgagc aatttaccca g
//
LOCUS       DASTSF010000451        12311 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1384027,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000451 DASTSF010000000
VERSION     DASTSF010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 12311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12311
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1384027"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /locus_tag="VFU15_13060"
     CDS             <1..224
                     /locus_tag="VFU15_13060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718764.1"
                     /translation="NGCTCLMKTWTYKNGKWDLLIDPFVILTGCVFFSDSLLQAYVFR
                     KDGAVFFLDARLDSAGGETLAGKKAVLEK"
     gene            554..1066
                     /locus_tag="VFU15_13065"
     CDS             554..1066
                     /locus_tag="VFU15_13065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718765.1"
                     /translation="MKTFTSLCFLFLSSVSFSANIFSHDSSDANVPYFDITGRMTIAD
                     KSVADYSVILYCDGQRTDSVFVDTKKDIDIHLGYGHDYAVRFSKAGCKDRILLVDTHL
                     PEGVSADYFSFLYSIEFLSEHAPSNTFDDFPVAYIGYDKTKKDFDYNRTYHKNIRVMP
                     GHPETQTASR"
     gene            1211..2215
                     /locus_tag="VFU15_13070"
     CDS             1211..2215
                     /locus_tag="VFU15_13070"
                     /inference="COORDINATES: protein motif:HMM:NF013030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEU4718766.1"
                     /translation="MKISASIYSTSRDLESVIRDLDAHRIDFFHVDCNDDPKVFEDIE
                     RIRSRSRTPVDLHIISKTPEKYFPLLEKTPVELVTFQYEDLPGKLEVPETITAKLGLA
                     ITANTDIDVFEQYSDRFDFILMMATIPGKSGGQFNKEIFRKIRLFKSRYPEKRIHVDG
                     GVNGEVSFILRNMGVYASVSGSFLFNAESLGFALLNLKTHENESHFLVKDFMMSFGEI
                     PLLKPGERSFEKILRSIEAFALGFTILAGGDNTLEGIVSNADVRKGLLKKINALSSIS
                     ENDLVNKNPVTVNENATVVELIRLIKSKKFPISYLPVVDNENKVTGALTFFNLVKGEA
                     "
     gene            2289..3287
                     /gene="aroF"
                     /locus_tag="VFU15_13075"
     CDS             2289..3287
                     /gene="aroF"
                     /locus_tag="VFU15_13075"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF006421.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEU4718767.1"
                     /translation="MIIELKNTVAEKRAQEMAQQLKAFCVHYDGKYVLITSSSVKELD
                     AKFQGEADRVTVLNDDMQLSSRAWKKETRTININGTVIGGSTNNTMLIAGPCSVESEE
                     QIGQSAQLLKELGISTLRAGAYKPRTSPYTFQGMGLDGLKLLAKMRDKYGLNIITEVR
                     DATQVEDIIEYADIIQIGAKSMYDHGILKRCGKTTKPVLLKRGFGTTLQEFLQCAEFI
                     LSGGNPNVILCERGIRTFETKTRFTLDLCGAAWMKQYSNLPVILDPSHAIGYAYGVPD
                     LARACCAMGVDGLLIEVHPNPKVAKSDASQQLNHDEFRALSSSLKNVAAAIGRKII"
     gene            3375..4031
                     /locus_tag="VFU15_13080"
     CDS             3375..4031
                     /locus_tag="VFU15_13080"
                     /inference="COORDINATES: protein motif:HMM:TIGR01670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HEU4718768.1"
                     /translation="MSLFAANLSFLLRKNGIDSAKLSAELGIKDLARPLPDEIPHIAN
                     RFSVTTDILLRTDIETREAQRSKNIRLVIFDIDGVLTDAGMYYTENGDELKKFNAKDG
                     YAIRQLKQRGIETGIISHGFNTKLIERRAERLHIKFLEVSQVPKKETLTKWCAQLDIT
                     PENVCYIGDDLNDEDIINTVGFSACPADAVDDVKNMVHVVLSKKGGEGCVREMIDLYL
                     "
     gene            4044..5234
                     /locus_tag="VFU15_13085"
     CDS             4044..5234
                     /locus_tag="VFU15_13085"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEU4718769.1"
                     /translation="MRKKSLSAFFIASLFCLVSLHAQPFTKKYIMAFLSCDSTCTGPQ
                     DHKVHLAESDDGTAWSAVPNFPVYNGSVPDPVIRGNKLYLYTPGKVRRYDNGTGTWDP
                     APSMVSIVDTAGNAVQFVDPSAIVDSSGNIVLFFLNSTGSTTDPAVCTSYPCVKHFDS
                     AIEVPSSDGTQFVMVPGDRYSLTLNSGSASDPDIFFDGTQYILYISRGAQLEAAHSSS
                     LQGTYTDFSSLPGGIISPQGGIGCGYYDALSAQYWTYTHTNLSGNTVIRQSVHSGFSS
                     QISSYSTVVSAATIGQPASTTTESPGFCANTFPTFVEEYPQDILHGNFFPDPVSGISY
                     LRLDRPVSNATLVISDVSGRIVKTAAFSGTEISLDCSMLQSGIYFYTLAAEDGSLRGK
                     FLVN"
     gene            5300..6097
                     /locus_tag="VFU15_13090"
     CDS             5300..6097
                     /locus_tag="VFU15_13090"
                     /inference="COORDINATES: protein motif:HMM:NF012340.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HEU4718770.1"
                     /translation="MKKYRLNIVPSRKQEEDWTLEKAARLGHFRPVKIVPGSVDLRES
                     WWKPGNQGQTGSCVGWSLADGVLRWHYVKKKKMRHDEKPSVRFIWMSAKEMDEYSDRP
                     TTFIDDSGTSLKTALDVARKFGCVKDSLLPFGRHELYAGDEDKIFKIASKYKIRSYYN
                     LVKSAGSKDKLNLFRRWLASGGGPIFTCLDVDIHWENALLTKGHLRKYNKRTANGGHA
                     VCIVGYTPDYFIVRNSWGASGWGDKGFAYAHNAYAQQAFKEAYGVVI"
     gene            6162..7712
                     /locus_tag="VFU15_13095"
     CDS             6162..7712
                     /locus_tag="VFU15_13095"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEU4718771.1"
                     /translation="MKNSRRYLLPFAVLFASFFALSLTNEKSAPAAQPAFITDSLDTY
                     ITREMKNWDLPGLAVAVVKDGQVVVSKGYGVTEMGTDKKVNDESLFMIASCTKAFTAT
                     SLAVLGQEKKLSLNDSVVKWLPGFHMYDPAVTPMVTIRDLLCHRLGFQTFQGDFLNWD
                     SNLSRAQIIDAIGRNKPVFGFRAQYGYCNAAFLTAGEIIPKATGKSWDDFVKERFFIP
                     LKMTRSSTTHDVIAHDANACVPHTRWNGKETKIAYDVVDNLGPAASVNSCVKDLSHWL
                     LMQLDSGRYGGNHIVPFAALAETRKPQTIIENRNPDYHFVSYCLGWTTADYHGKQLFW
                     HNGGASGFLSTVCFIPELNLGIVVLANSDNNNLFNALRQQIIDAYMGLPYKNYSALYL
                     PRYKKFWADDDAQVAKWNADVAKNPKLPVDLQAFAGMYENPVYGKMNVVVEENKLSVS
                     FEHHPNIIGKLEYMGDSTMLCTFFSPLWGIKPAPFVIKDGKVESITISVNGEVDAMPY
                     EFRHNDKP"
     gene            7820..8404
                     /locus_tag="VFU15_13100"
     CDS             7820..8404
                     /locus_tag="VFU15_13100"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU4718772.1"
                     /translation="MKPEQTETYKIISRGGKESVSLLYERYGKKLFAYAVHSWKTTED
                     ESWELVYKTLYRVMETAGKYSFDSEKNFSSFVFKVFVNFLRQHYRDVKRKNEHLGFVE
                     MNDSVLQQNSAPEKEDAPETENMARLKTELEKLEDWERMLLLLRSQDMPYADIAKYVD
                     RPEEHLKVYYQRLKASLAKKLNETTHSKNTTDEN"
     gene            8394..10562
                     /locus_tag="VFU15_13105"
     CDS             8394..10562
                     /locus_tag="VFU15_13105"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HEU4718773.1"
                     /translation="MRTDSPQQSGMNGLDSLLRQFVLEGGDDPFTQKTLDMNAEFVFS
                     QTAAAPSDARGAALVEKLAEAFPRKGNSGRFRLSGAIFILLISVASWMYFSSSPQVPS
                     ATGSSPGNKSSSPLPPAPSGDTSSVTEITVKNHALAARDSSGIASANDSVSGSDTAHV
                     SKDPSHHHYANDNRKIDLDEKWYEGKYPVNVPGPAFSAKASPENFHVMSGSRAIDSLD
                     NAGAFSAGRKTTFRSVYYSGIPFKSGLNDMQYLNFGESVFKDSLSRFYWNFPWTMIGQ
                     LDSDAVTVATKKNKDGVASAGLFASRLPEKGIRLVMEPFYFRKYEVTNKEYREFLEWV
                     RASNGYAGKPLRTFTADTVPVTDTSTLSGYDIKIKGKTYRVHITSFILLEDYHEVYNY
                     VFFNDSSEAVRSLGKNSLYVYPDTLCWTTDFTFSYNEPMANMYFWHPAYDNYPVVGVS
                     WYQALAFLDWKTHFHQQSLDAAGVPYKIEYTLPSDVEWDLVSTALPLKDKAVPLAARE
                     CCNDWITDLALSYSGHDDPYQRFNYLKRLLTKDENFNGDYIADGYFFTGPADLTQSSD
                     SYKEYKLMGRFPRTDNADLGNEKDLDRNLDTRHYDILGISWMDGNVSEWMMESYAENW
                     EPFFKKHLLVMDADTSEAAQLAEQIEMLYDKGNAKNGRLVRGANWYDERFAGKPGSGV
                     NEAGISAKRFIDPQEQHCTVGFRYVLHVKYKDEKARLSKN"
     gene            complement(10763..11488)
                     /gene="recO"
                     /locus_tag="VFU15_13110"
     CDS             complement(10763..11488)
                     /gene="recO"
                     /locus_tag="VFU15_13110"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEU4718774.1"
                     /translation="MLQSTRGIVLHTIPYSDSRIIARIYTERSGFTSYMLSVSRSKKG
                     KIRGNILQPLTQIEIVADHREKNAMQTAREISCPEPYLHLQDDIVKTSIALFLAEVLY
                     KSVKEEEPNPPLFSFLTNAFHILDLHEEGVANFHLVFLVQLTRYLGFYPQQRSFEGKT
                     FFDLKDGVFRSAIPPHPLYLEEAESRLLEELMQLSFDNMQTLALTGEWRGKMVKHLLQ
                     YYQLHLSSMHDVKSHHVLEAVLS"
     gene            complement(11500..>12311)
                     /locus_tag="VFU15_13115"
     CDS             complement(11500..>12311)
                     /locus_tag="VFU15_13115"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HEU4718775.1"
                     /translation="QKWMILPRGSGMLVFDDNGTIDNPSDDRVTRLGFTAGNGGIVGT
                     DVYSMAEDQDGEIWVGTDQGISVFYCAENIFTPGACDAQQILIQQDGYTQILMGTQSV
                     TCIAIDGANRKWIGTEEGGVFLMSADGQKQLAHFDVTNSPLLSNDITSLAINQKTGEI
                     FFGTTKGLVSYRGEAIEGQDQMGDVYAFPNPVTHDYHGPIAITGLVKDADVRIADVTG
                     NVVYQTKALGGQAIWDGNNFKGERAATGVYLVFITNEDGTQKAITKILLIN"
BASE COUNT         3011 a         3415 c         3100 g         2785 t
ORIGIN      
        1 ggaacggctg tacctgcctg atgaaaacct ggacgtacaa gaacgggaaa tgggacctgc
       61 tgattgaccc gttcgtgatt cttaccggct gtgtgttttt ttcggacagc ctgctgcagg
      121 catacgtttt ccggaaagac ggcgctgttt tttttctcga cgcgcgtttg gattcagccg
      181 gaggcgaaac gcttgccggg aaaaaagctg tactggaaaa atgaaccggg acggaaaagt
      241 atcggatgtc tgccgagcat gcagcaggaa aaacacgctc ctctgaaagc aaaacgcggg
      301 tttaaatatg attacggcag ctgcggacgg ccggagcatg attgtcagta tgcgattccg
      361 ttcggtttat gaaacagttc tgcgtcccgg gttgagcatt ctgcattgat ccgctttccg
      421 gtatgaagat tcaccatccg cggggttcgc caacaaaaaa tcggtttttc ttacatcggc
      481 aaacttccgg tgcttcccgg tttccacacg tctgcgcatg gaaatagttt tgatccatca
      541 aatccaaaac accatgaaga ccttcacctc gctctgcttc cttttcctca gctccgtttc
      601 tttttccgcg aatattttct cgcacgacag ttcggatgcc aatgtcccgt acttcgacat
      661 caccggccgc atgacgatcg ccgacaaatc cgttgcggat tattccgtga tcctttattg
      721 cgacgggcag aggacggact ccgttttcgt cgatacgaaa aaagacatcg acatccatct
      781 cgggtacggt cacgattatg ccgtgcgctt ttcaaaggcc ggctgcaaag accgcatcct
      841 gctggtcgac acgcatctcc cggaaggcgt ttccgctgat tatttcagct tcctgtattc
      901 gatcgaattc ctttccgaac atgcgccttc gaatacgttc gatgattttc ccgtggctta
      961 catcggttac gataaaacga aaaaagattt cgactacaac cgcacttatc ataaaaacat
     1021 ccgcgtcatg cccggccacc cggaaacgca aacagcttcc cgctgatgtt tttcaaactc
     1081 atgaggaccc cgaaagacca tcgccgggac accgaagccg cttccctcgc gtcttcctgt
     1141 cccggcggtt atgttttaca aagcccctca tcgcacacag attgcttacc tttgacaccg
     1201 gaattcccac atgaaaattt cggcgtccat ctattccact tcccgcgatc ttgaaagcgt
     1261 gatccgcgat ctcgacgcgc accgcatcga ttttttccat gtcgactgca acgacgaccc
     1321 gaaagttttt gaagacatcg aacgcatccg ctcccggagc cggacgccgg tggacctcca
     1381 catcatttcg aaaacgccgg aaaaatattt tccgctgctc gaaaaaacgc ctgttgagct
     1441 cgtcacgttc cagtacgaag accttcccgg gaagctggag gttccggaaa ccatcaccgc
     1501 aaagctgggc ctcgccatta cggccaacac ggatatcgat gttttcgaac aatattcaga
     1561 ccggttcgat ttcatcctga tgatggccac cattcccggc aaaagcgggg gacagttcaa
     1621 caaggaaatt ttccgcaaga tccgcctgtt caaaagccgt tacccggaaa aacgcatcca
     1681 cgtcgacggc ggcgtgaacg gtgaagtgtc gttcatcctg cgcaacatgg gcgtgtatgc
     1741 cagcgtgtcg gggtcgttcc tgttcaacgc agaatcgctc ggcttcgccc tgctgaacct
     1801 gaaaacacac gagaacgaat cgcatttcct cgtgaaggat ttcatgatga gcttcgggga
     1861 aattcccctg ctgaaacccg gggaaagaag cttcgagaaa attctccgca gcatcgaagc
     1921 gtttgcgctc ggctttacca ttctcgccgg cggcgacaat acgcttgaag ggatcgtcag
     1981 caacgccgat gtacggaaag ggctgctgaa aaaaataaac gcgctttcct ccatctcgga
     2041 aaatgacctt gtgaataaaa atcctgtcac ggtgaacgaa aacgcgaccg ttgtcgaact
     2101 catccggctt atcaaatcga aaaaattccc gatcagctat ctgcccgtgg tggataatga
     2161 aaataaggtc accggcgcgc tgacgttctt taacctggtg aaaggagaag cataaatcaa
     2221 ttccgggttt tgaattctga atgaacgcta tattcagaac ccggaatcaa gcattcaaaa
     2281 ttcaaacaat gatcatcgaa cttaaaaata ccgtagccga aaaacgcgcg caggaaatgg
     2341 cgcagcagct gaaagcattt tgcgtgcact acgacggaaa atacgtgctc atcacttcgt
     2401 cgtccgttaa agagctcgac gcaaaattcc agggcgaagc ggatcgcgtc accgtgctga
     2461 acgacgacat gcagctttct tcccgcgcct ggaaaaaaga aacgcgcacg ataaacatca
     2521 acggaacggt tatcggcggc agcacgaaca acacgatgct catcgccggt ccgtgctcgg
     2581 tggaatcgga agaacagatc gggcagagcg cgcaattgct gaaggaactg ggcatttcca
     2641 cgctgcgcgc gggagcctac aaaccgcgca catcgccgta cacgttccag ggaatgggcc
     2701 tcgacggact gaagctgctg gcgaaaatgc gcgacaaata cggactgaac atcatcacgg
     2761 aagtgcgcga tgccacgcag gtggaagata tcatcgagta cgccgacatc atccagatcg
     2821 gtgccaaatc gatgtacgat catggcatcc tcaagcgctg cgggaaaacc accaagcccg
     2881 tgctgctcaa gcgcggtttc ggcaccacgc tgcaggaatt cctccagtgc gcggaattca
     2941 ttctgtcggg tggaaatccc aacgtcattc tctgcgagcg cggcatacgc acgttcgaga
     3001 ccaaaacacg tttcacgctc gatctttgcg gagcggcgtg gatgaaacaa tattcgaatc
     3061 ttccggttat cctcgatcct tcgcacgcga tcggctacgc gtatggcgtt cccgacctcg
     3121 cgcgcgcctg ctgcgccatg ggcgtcgacg ggctgctcat tgaagtccat ccgaatccga
     3181 aagtggcgaa aagcgatgcg tcacagcagc tcaaccacga cgaattccgt gcgctttcct
     3241 cctcgctgaa aaacgtggcc gcggccatcg gaaggaaaat catttagctt tgcctgtctt
     3301 cgcacattcg ggtattgccg ggcggaggag tccggaacct ggcgaacgaa tggcatgaaa
     3361 tgtgacaaaa ccaaatgagt cttttcgccg ccaatctttc ctttcttctc cgtaaaaacg
     3421 gcatcgattc cgcgaagctt tccgccgagc tcggcatcaa agaccttgcg cgcccgcttc
     3481 cggatgaaat tccgcacatt gcgaaccgtt tttccgttac cacggatatc cttctccgca
     3541 cggacatcga aacgcgcgaa gcacaacgtt cgaagaacat ccgcctcgtg atcttcgaca
     3601 tcgacggcgt gctgacggat gcaggaatgt attacactga aaatggcgat gagctgaaaa
     3661 aattcaacgc gaaggacggt tacgccatcc gccagctgaa acagcgcggc atcgaaaccg
     3721 gcatcatcag ccacgggttc aacacgaagc tgatcgaacg gcgcgccgaa cgcctgcaca
     3781 taaaattcct cgaggtttcg caggtgccga aaaaagaaac gctgacgaaa tggtgcgcgc
     3841 agctggacat cacgccggaa aacgtctgtt acatcggcga tgacctcaac gacgaagata
     3901 tcatcaacac cgtcgggttt tccgcctgtc ccgccgatgc ggtcgacgac gtgaagaaca
     3961 tggtgcacgt cgtgctttcg aaaaaaggag gagaaggctg tgtgcgggaa atgatcgacc
     4021 tgtacctgta aattccaccg gccatgcgaa aaaaatctct ctccgcattt tttatcgctt
     4081 cgcttttctg cctcgtttct cttcacgcgc agcccttcac aaaaaaatac atcatggctt
     4141 ttctttcctg cgattccacg tgcacgggac cgcaggacca caaagttcat cttgccgagt
     4201 ccgacgacgg taccgcatgg agcgccgttc cgaattttcc cgtgtataac gggagcgttc
     4261 ccgatcctgt catccgcgga aacaagcttt acctctacac gcccgggaaa gtgcggcgct
     4321 acgacaacgg cacgggcacg tgggatccgg cgccgtcaat ggtttccatt gtcgataccg
     4381 cgggaaatgc cgtccagttc gtcgaccctt ccgccatcgt cgacagcagc gggaacatcg
     4441 tgctgttctt tctcaactcg acgggaagca caaccgatcc cgcggtctgc accagctatc
     4501 cctgcgtgaa gcatttcgat tcggcgatcg aagttccgtc gagtgacggc acgcagttcg
     4561 tcatggttcc cggcgacagg tattcgctca cgctgaacag cggttccgct tccgatccgg
     4621 acattttctt cgacggcacg caatacatcc tttacatttc acgcggagca cagctcgaag
     4681 ccgcacattc ctcctcgctg caggggactt acacggattt ttcatcgctg ccgggcggga
     4741 tcatttctcc gcagggcggc atcggctgcg gttattacga tgcgctttcc gcacaatact
     4801 ggacctacac gcacacaaat ctttccggga acacggtgat ccgccaatcc gttcacagcg
     4861 gtttttcctc gcagatctct tcctatagca cggttgtttc cgcggcaacg atcggtcagc
     4921 cggccagcac caccacggaa agcccgggtt tttgtgcgaa tacattcccg actttcgttg
     4981 aagaatatcc gcaggatatt ttgcacggca acttcttccc ggatcccgtt tccggaattt
     5041 cctacctgcg cctcgaccgc ccggtgagca atgcaacgct ggttatttcc gacgtcagcg
     5101 gccgtatcgt gaaaacagct gcattctccg gaacggaaat ttcccttgac tgctcgatgc
     5161 ttcaaagcgg aatttatttt tacacgttag ctgctgaaga tggttccctg cgcggaaaat
     5221 tccttgtaaa ttgagcattg ctttataaaa gcattactca tgtccgttca acaaacccgt
     5281 taacccaact tccccgatca tgaaaaaata caggctgaat attgttcctt cccggaaaca
     5341 ggaagaagac tggacgctgg aaaaagcggc ccgtctcggg catttccggc cggtgaagat
     5401 cgttcccggt tccgtcgacc tgcgcgaaag ctggtggaaa cccggcaacc agggacaaac
     5461 aggctcctgt gtgggctggt cgctcgccga cggcgtgctg cgctggcatt acgtgaaaaa
     5521 gaaaaaaatg cgccacgatg aaaaaccatc cgtgcgcttt atctggatgt cggcgaaaga
     5581 gatggatgaa tattctgatc gacctaccac gtttatcgac gattccggca ccagcctgaa
     5641 aacggcgctc gacgttgcgc ggaaattcgg atgtgtaaag gattccctgc tgccttttgg
     5701 ccggcatgag ttgtacgccg gcgacgaaga caaaattttt aagatcgcat caaaatataa
     5761 gatccgcagc tattacaacc tcgtgaaatc ggccggcagc aaagacaagc tcaacctgtt
     5821 ccggcgctgg ctcgcttcgg gcggcggacc tattttcacc tgtctcgatg tggatattca
     5881 ctgggaaaat gcactgctca cgaaaggaca tctccgcaaa tacaacaaac gcacagcaaa
     5941 tggggggcac gccgtttgca tcgtcggcta tacgcccgat tatttcatcg tgcgcaattc
     6001 ctggggggca agcggctggg gcgacaaagg tttcgcgtac gcacacaatg catacgcgca
     6061 gcaggcattc aaagaagcct acggagtggt gatttgaaac cctttcccga catcccggcc
     6121 gataatctcc cgcagcatta ttgaccatta tttttgttct catgaaaaat tcccgtcgtt
     6181 atcttttgcc ttttgcggtc ctcttcgctt ctttcttcgc gctttcgctg acgaatgaaa
     6241 aaagtgctcc cgccgcacaa ccggctttca tcaccgattc gctcgacacg tacatcacgc
     6301 gcgaaatgaa aaactgggac ctccccggac ttgctgtcgc tgtggtgaaa gacgggcagg
     6361 tggtggtttc caaaggctat ggcgtaacgg aaatgggaac ggacaaaaaa gtgaacgacg
     6421 aatcgctgtt catgatcgcg tcctgcacga aagcatttac cgccacgtcg ctcgccgtgc
     6481 tcgggcagga aaaaaagctt tcgctgaacg attcggtcgt gaaatggctg ccgggatttc
     6541 acatgtatga tcccgctgtc acgcccatgg tgaccattcg cgacctgctg tgccaccgcc
     6601 tcggcttcca gacgttccag ggcgattttc tcaactggga ttcgaacctt tcccgcgcgc
     6661 agatcatcga tgcgatcggt cgcaacaagc cggtgttcgg cttccgcgca cagtacggct
     6721 attgcaatgc ggcgttcctc accgccggcg agatcatccc gaaagccacc gggaaatcct
     6781 gggacgattt cgtgaaagag cgctttttca ttccgctgaa aatgacgcgt tcatccacga
     6841 cgcacgatgt gatcgcgcat gatgcgaacg cctgtgtgcc gcatacacga tggaacggga
     6901 aggaaacgaa gatcgcgtac gacgtggtgg ataatctcgg gcccgccgct tctgtcaact
     6961 cctgcgtgaa agatctttca cactggctgc tcatgcagct cgacagcggc cgttacgggg
     7021 gaaatcacat cgttccgttt gctgcacttg cggaaacacg caagccgcag acgatcatcg
     7081 aaaaccgtaa ccccgattat cattttgttt cctactgcct cggctggacc accgccgact
     7141 atcacgggaa acagctgttc tggcacaacg gcggcgccag cggatttttg tcgaccgttt
     7201 gtttcattcc ggaactgaac ctcgggatcg tcgtgctcgc caacagcgac aacaacaacc
     7261 tcttcaacgc gctccgccag cagatcattg acgcgtacat gggccttccc tataaaaatt
     7321 acagcgcgct gtatttgccc cgctacaaaa agttctgggc ggacgacgat gcacaggtgg
     7381 ccaaatggaa tgcagacgtt gccaaaaacc cgaagcttcc cgtcgacctt caggcgtttg
     7441 ccggcatgta cgaaaacccg gtttacggca aaatgaatgt cgtcgtcgaa gagaacaaat
     7501 tgtccgtctc tttcgagcac cacccgaaca tcatcggcaa gctcgaatac atgggcgatt
     7561 cgacgatgct ctgcaccttc ttctccccgc tctgggggat caaaccggcc ccgttcgtga
     7621 tcaaagacgg gaaagtggaa tccatcacca tttccgtgaa cggggaggtc gatgctatgc
     7681 cgtacgaatt ccggcacaat gacaaaccct gattttccgc ttcccggagg gtagtccgac
     7741 attcagaatt gcctcatccg ttccgtttgt tacctgtccg gattatacat ttaaagcgca
     7801 ggaataagac cggaagcgag tgaagcccga acagacggaa acatacaaga tcatctctcg
     7861 cggcgggaaa gaaagcgttt cgcttcttta cgaacgctac gggaaaaaac tgttcgcata
     7921 cgccgttcac agctggaaaa ccacggagga tgaatcctgg gaactggtct acaaaacgct
     7981 gtaccgcgtc atggaaaccg ccggaaaata ttcgttcgat tccgaaaaga atttcagctc
     8041 ttttgttttt aaagtgttcg tgaatttcct ccggcagcat tatcgtgatg tgaaaagaaa
     8101 aaatgaacac ctcggtttcg tggaaatgaa cgattccgtt ttgcagcaga attccgcccc
     8161 cgaaaaagag gatgcgcctg aaacggagaa catggcgcgg ctcaaaacag aactggaaaa
     8221 gctcgaagac tgggaacgca tgctgctcct gctgcgcagc caggacatgc cctacgccga
     8281 catcgcgaaa tacgtggacc ggcccgaaga gcatctcaag gtgtattacc agcgcctgaa
     8341 agcgtcgctc gcgaaaaaac tgaacgaaac aactcactca aaaaatacga cggatgagaa
     8401 ctgacagccc gcaacagtcc ggaatgaacg ggctggattc ccttctccgt caatttgtcc
     8461 tcgaaggcgg ggacgatcct ttcacgcaaa aaacactgga catgaacgca gaattcgttt
     8521 tctcccaaac cgccgcagct ccgtcggatg cgcgcggcgc ggcgctcgtc gaaaagctgg
     8581 ccgaagcctt tccccgcaag ggaaatagcg ggcggttccg cctatccggc gcaattttta
     8641 tcctgctgat ctctgttgct tcctggatgt atttttcttc ctcgccgcag gttccttccg
     8701 caacgggttc ctcgccggga aataaaagtt catcgccgct tcctcctgcc ccttccggcg
     8761 atacgtcttc tgtgacggaa ataacagtga aaaatcatgc gctcgctgca cgtgattctt
     8821 ccgggatcgc atcggccaac gattccgtca gcggctccga cacggcgcat gtttcaaaag
     8881 atccgtcgca tcaccattac gcgaatgata acaggaaaat tgatcttgat gaaaaatggt
     8941 acgaaggaaa atatccggtg aatgttcccg gaccggcatt ttccgcgaag gcatcacccg
     9001 agaattttca cgtgatgtcc ggttcgcgcg cgatcgactc gctcgataat gcgggcgcat
     9061 tttcggccgg caggaagacc actttccgtt ccgtgtatta ttcggggatc cccttcaaaa
     9121 gcgggctgaa cgacatgcag tacctgaact tcggcgaatc cgtgtttaaa gacagccttt
     9181 cccgtttcta ttggaatttt ccatggacca tgatcgggca gctggattcg gacgccgtaa
     9241 ctgtggccac gaaaaaaaat aaagacggcg tagcttctgc agggcttttt gcttcacgtc
     9301 tgcctgaaaa aggcatccgt ctcgtgatgg aaccctttta tttccggaaa tatgaagtaa
     9361 cgaacaagga ataccgtgaa ttcctcgagt gggtgcgcgc atcgaacggc tatgcgggaa
     9421 aacctttgcg cacgttcacc gcggatactg ttccggtaac ggatacaagc acgctgtcgg
     9481 gatacgatat aaaaatcaaa ggaaaaacat accgggtcca catcacttcg ttcattctgc
     9541 tcgaggatta tcatgaagtg tacaattacg ttttcttcaa cgacagcagc gaagccgtga
     9601 gatcactcgg aaaaaattca ctctatgttt atcccgatac gttatgctgg acaaccgatt
     9661 tcacgttcag ctacaacgag ccgatggcga acatgtattt ctggcacccg gcgtatgaca
     9721 actatcctgt ggttggcgtg agctggtacc aggcgctggc gttcctcgac tggaaaacgc
     9781 atttccacca gcaatcgctc gatgctgccg gcgttccgta caagatcgaa tacacgcttc
     9841 catccgatgt agaatgggat ttggtctcga ccgccctgcc gttgaaagat aaagctgtgc
     9901 cgcttgctgc gcgtgaatgc tgcaacgact ggatcacaga tctcgctctc agttattctg
     9961 ggcacgatga tccttaccag cgtttcaatt acctgaagcg ccttcttaca aaagacgaaa
    10021 atttcaatgg cgattacatt gcggatggtt acttcttcac cggacccgcc gatcttacgc
    10081 aatcgtcgga cagctacaag gaatacaagc ttatgggacg tttcccgcgg acggataacg
    10141 ccgatctcgg gaatgaaaaa gatcttgacc gcaatctcga tacacgacac tatgatatcc
    10201 tgggaatttc ctggatggat ggcaatgttt cagagtggat gatggagtcg tatgcggaga
    10261 actgggagcc gttttttaaa aaacacctgc ttgtgatgga tgccgatact tcggaagccg
    10321 cgcagctggc cgaacagatt gagatgctgt acgacaaagg caatgcgaaa aatggacgcc
    10381 ttgttcgtgg cgccaactgg tacgacgaac gttttgccgg gaaaccggga agcggtgtaa
    10441 acgaagccgg cataagcgcc aagcgtttca tcgatccgca agaacagcat tgcaccgtcg
    10501 gattccgcta tgtgcttcac gtgaaataca aggatgaaaa agcgcgcctc tcgaaaaact
    10561 gaactgcgcc tttctctgtc gggcgcaagt caagaccatt gtcaggtcga gcgaaagtcg
    10621 agaccaccgt caggtcgagc gcaaatcgag accactgtca ggtcgagcgc aagtcgagac
    10681 cactgtcagg tcgagcgcaa gtcgagacca ccgtcaggtc gggcgagagt cgagaccact
    10741 tctcaaacaa atcagctccg cctcacgaca acaccgcttc cagcacatga tgtgatttta
    10801 catcgtgcat cgaggaaaga tgcagctggt aatactgcag caggtgcttc accattttac
    10861 cgcgccattc tcccgtaagc gccaatgtct gcatgttgtc aaaagaaagc tgcatcagtt
    10921 cttccagcag gcggctttcc gcttcttcga gataaagcgg gtgtggcgga atagcgctgc
    10981 ggaacacacc gtctttcagg tcgaagaatg tttttccttc gaatgacctc tgttgcgggt
    11041 aaaaaccgag ataacgcgtc agttgaacca gaaaaacgag gtgaaaattc gcaacgccct
    11101 cttcgtgcag gtccaggata tgaaaagcat tggtaagaaa cgagaacaac ggcggattcg
    11161 gctcttcctc tttcaccgat ttgtacagca cttccgccag aaaaagcgca atgctcgttt
    11221 tcacgatatc gtcctgcagg tgaagatagg gttccgggca ggaaatttcc cgcgcggtct
    11281 gcatggcgtt cttttcgcgg tggtccgcta cgatctcgat ctgcgtaagc ggctgcagga
    11341 tattgccgcg tatctttcct tttttgctcc gcgaaacaga aagcatgtac gaggtgaacc
    11401 cggatctttc cgtgtaaatc ctggcaatga tgcggctgtc ggaatacggg atggtatgaa
    11461 gaacgatgcc acgcgtggat tgcagcattg gtcttccgtt tagttgatga gaaggatctt
    11521 cgtgatcgct ttttgcgttc cgtcttcatt ggtgatgaaa acgagataca cgccggttgc
    11581 cgcacgttcg cccttgaagt tgtttccgtc ccagatggcc tgcccgccca gcgctttcgt
    11641 ctggtaaacc acgttgccgg ttacgtccgc aatgcgcaca tccgcatctt tcaccagccc
    11701 ggtgatcgcg ataggtccgt gataatcgtg cgttacggga ttgggaaacg cataaacgtc
    11761 acccatctgg tcctgcccct cgatggcttc tccccggtaa gaaacaagcc ctttggttgt
    11821 tccgaaaaag atctcccccg ttttctggtt aatggccagg ctcgtgatgt cgttcgaaag
    11881 cagcgggctg tttgttacat cgaaatgcgc gagctgtttt tgaccgtcgg cagacataag
    11941 gaaaacgccg ccttcttccg tgccgatcca tttccggttg gcaccatcaa tggcaatgca
    12001 cgtcacggac tgcgttccca tgaggatctg cgtgtatccg tcctgctgaa taaggatctg
    12061 ctgggcatcg caggcgcccg gtgtgaaaat attttccgcg caataaaaaa cggagatccc
    12121 ctggtcggtc ccaacccata tttccccgtc ctggtcttcc gccatcgaat aaacatccgt
    12181 tccgacgatt cctccgttgc cggccgtaaa acccaggcgt gtcaccctgt cgtccgaagg
    12241 attgtcaatg gttccattgt cgtcaaaaac aagcattccg cttccgcgcg gaaggatcat
    12301 ccacttctgc c
//
LOCUS       DASTSF010000452        14095 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_745744,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000452 DASTSF010000000
VERSION     DASTSF010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 14095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14095
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_745744"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1619
                     /locus_tag="VFU15_13120"
     CDS             <1..1619
                     /locus_tag="VFU15_13120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718776.1"
                     /translation="TFLGSGTTIVNEKKGIEQWAGAGWTGQPLIVDECGKKFLIQGCF
                     DHHLKKIDAQTGDVKWEYAYDDILKGTGTIWKNDSAADPENRIEIIQGSRKGVNSPLC
                     GAHAYSFRAVSYFTGKELWRMDVEHTASYSRDCDASALVISDTAYIGLENGKFKWFDP
                     GKKTIPSDSFAAPDVFGCTDLYNREDAAHHGGNLVTESSPAHIGNHIYITAGSGHVYG
                     FNLATKIIDWDFFIGSDMDGSPVVTSDSCLLIPVEKQYIAGNGGVFKLDPSKSPDNCV
                     QWYFPSADFSFASWEGGVIGSVAVNDRYNDGEFPRLASFMGIDGYLTVVKYDHVRKDT
                     LVKGPDGKTVYPCPEVVFRKRTGPSISTPLFVNDKLVAAGYSGICLFRYDRGGFFYEL
                     AFHPGIFEATPSADAGKIFIASRDGFLYCFGDAGTGTGKEALSHNTPAPVTQAVAAKK
                     KKDAPPAVQKKEERNTFHLVAGAFRVKQNAVNFKKQWEAKGVSGSLIEAPMDLYYVSL
                     FDTESKEAADRKHDELMKQFGADTWIYTGN"
     gene            complement(1616..2902)
                     /locus_tag="VFU15_13125"
     CDS             complement(1616..2902)
                     /locus_tag="VFU15_13125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718777.1"
                     /translation="MLRILTLLSCAVFFLSCGNNPKKSESGSTKNDQFTDRFRKDVLT
                     LRHVGLLNNIPDKTLDSLIPVYAKDTLNGLKDLLVYSGEAMKLDLRENGKAPHEVYDR
                     LCNEIGKRYPELRVDSTRYHYLADYPEDHDTDWVLMRMKAGSDVFLQKLYWFRDWPID
                     ETFCKVYNTMLAQQNKSYRMFMISYRCTNCVNPWDDENVKFDINTIGLIMLTKAQEDS
                     LMPMWDLALDPEEEFSTMSPHQLDSMFTRLESSGLVTAKDKPWYDAKKEEIRENTIYG
                     SEAFYDYIDTLFTTVSFDTINDYNPYAEMLKAMTHISRGHFNPGIVSDTTTDANFTDH
                     TVRFSLNGKVYESEEMQRGTVLCTGLIDDVNKAMEEQKAGGAFYTVLTRDKIAIIVFI
                     EDDKLEKVKSSGLFRDMQKGTPAYLRDLYNNQPELD"
     gene            complement(2907..3521)
                     /locus_tag="VFU15_13130"
     CDS             complement(2907..3521)
                     /locus_tag="VFU15_13130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718778.1"
                     /translation="MQKTILSAFLLFALASCSNDKSTKVPGDSSVTDTGKVAVSDTAP
                     KDECAIRKKRIPKNYPQDSIDKDLIGLSRCGIDSFDYLYVVPNLFPAWLSERRVVGDT
                     VTYGDFIEHLEKFRTTDSYYKLHLKVATLDSLKTIPFQKKDIYRMKPLLGQLGFTEGE
                     WDMFSGFAVTYPVPDPKHFSWGDMLDAFEKYKSTRPPLPEDQIN"
     gene            complement(3580..4092)
                     /locus_tag="VFU15_13135"
     CDS             complement(3580..4092)
                     /locus_tag="VFU15_13135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718779.1"
                     /translation="MLKKILVSVFILLPFFLAGQADTAKFSRSLMPMEKDISLLLGIN
                     YWHWTYGEVGVAKNSFTLAGVHPFASAVFISDELKMDDHHFVMGPKIGGWIAGGASAM
                     ALGINAIYYTDFSAGSLRLRPEIGIGMGPFKVVYGYNLRLTGKYFPYVNSNVVSAVFL
                     IGLKKGTTAM"
     gene            complement(4153..4491)
                     /locus_tag="VFU15_13140"
     CDS             complement(4153..4491)
                     /locus_tag="VFU15_13140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718780.1"
                     /translation="MDQQKHQELRLLASDQHARGVENHHIEENLQKAGATPEMTAHIM
                     KEIKTIDFIRRKKRGFRSVTIGSFLLVFGFLITLLLYHSGSNINYAMYSVTIAGVVML
                     LWGMIDIMGW"
     gene            4640..5881
                     /gene="pepT"
                     /locus_tag="VFU15_13145"
     CDS             4640..5881
                     /gene="pepT"
                     /locus_tag="VFU15_13145"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016847737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HEU4718781.1"
                     /translation="MEIKHTVLERFLKYVQVDTQSDPDSPTCPSTEKQKDLGKILVEE
                     LLAMGVKDAHMDANGYVYATIPSNTKNKVPAICFCSHMDTSPDCSGKNVKPVVHKNYD
                     GKDIVLPADKSQVIRFAEHEALAGQLGNDIVTADGTTLLGADNKAGLAEIMDAAYYFM
                     THPDVRHGDIKILFTPDEEIGRGVDKADLKKLGAEFGYTMDGETVGHLEDETFSADGA
                     SLIIHGVSTHPGFAKGKMENALKIAGRILAELPRTTCTPETTEKMEGFIHPTSVNGTL
                     EQATINFILRDFSEEGLRDKEKTLSSVIRTVMKDYPKSTYQLVVKEQYRNMKEVLDKH
                     PRVVDYALEAIRRTGIEPVRSSIRGGTDGSRLSFMGLPCPNIFAGEHAFHSKQEWVSV
                     QDMEKAVQVIIHLVMIWEEKG"
     gene            complement(5951..6604)
                     /gene="fsa"
                     /locus_tag="VFU15_13150"
     CDS             complement(5951..6604)
                     /gene="fsa"
                     /locus_tag="VFU15_13150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013307258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEU4718782.1"
                     /translation="MKFFIDTADLAQIKEAQELGVLDGVTTNPSLMAKVGISGEAAVM
                     KHYVDICNIVTGDVSAEVISTDFDGIVREGEKLAKLHKQIVVKVPMIKEGVKAIRYFS
                     SKGIRTNCTLVFSPGQALLAAKAGAAYVSPFIGRLDDVSTDGLNLIAEIREIYDNYGF
                     ATQILAASIRHPMHIVDCAKIGADVATCPLSAIMALLKHPLTDNGLAQFLADAKKFS"
     gene            complement(6705..8339)
                     /locus_tag="VFU15_13155"
     CDS             complement(6705..8339)
                     /locus_tag="VFU15_13155"
                     /inference="COORDINATES: protein motif:HMM:NF025627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004062 - aryl sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aryl-sulfate sulfotransferase"
                     /protein_id="HEU4718783.1"
                     /translation="MKITLPGLLLLLCPFVHSQTPTVGLIQHTGGEDGGYVLFAPISD
                     TTTYLIDKCGKEIHSWTSAYRPGQSAYFQADGTLLRAGITNNPDFVAGGAGGIIEKLD
                     WNSNVLWSYAVSDTSQCQHHDICALPDGNILVIAWEKKTQAEAIAAGRNPAFLGSELW
                     GEKIVELQPVGNNSANIVWEWHLWDHLIQHYDSTKANYGIVGNNPQLVNINYSGSTAA
                     DWAHFNSIDYNPALDQIVVSVHNLNEIWVIDHSTTTAEAAGHTGGNSGKGGDILWRWG
                     NPAAYNHGNASNKMFFGQHNAHWITPGLPHENEIMVFNNGSGRPAGNYSTVEIISPPV
                     DSAGNYSSSLPYLPAAQDWIYADSVPANFYATNISGAQQLANGDVLICNGPAGTFFEI
                     DSTMHTVWKYINPVSGTGILSQGSTPTGNLVFRSPFYYSSYSGFNSVALSAGQPIELN
                     PYPYTCDLTGIKKTGDENVFSIYPNPSDDVVHISSGHDPMKSLCVIDLSGRVAENIAV
                     NGTSYDLPVNALSPGIYFLQVTTSSGASVQRKLIVN"
     gene            complement(8421..8978)
                     /locus_tag="VFU15_13160"
     CDS             complement(8421..8978)
                     /locus_tag="VFU15_13160"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU4718784.1"
                     /translation="MNIPPLFLETERLLVRNYRITDAADLFSLVNGNREELKEYFPNV
                     VENLSSVMAARKYILLRNRERSAAISIFSGVFLRKEEKLIGQIIVKDINQRVPKGEAG
                     YFLAKEFYGNGFASEALKKVTDYFLGEQKFAKLMCRIENRNSASRKVAEKAGYTFSGT
                     LRNDFRTLEGRLMDCEVWEKTSSPG"
     gene            complement(9032..9124)
                     /gene="ffs"
                     /locus_tag="VFU15_13165"
     ncRNA           complement(9032..9124)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VFU15_13165"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            9252..9788
                     /locus_tag="VFU15_13170"
     CDS             9252..9788
                     /locus_tag="VFU15_13170"
                     /inference="COORDINATES: protein motif:HMM:NF025227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HEU4718785.1"
                     /translation="MLKHSLIYGVVISFAVIALALGIYLSGVTNSATNYLSYLIYMGI
                     IIYAAKNYRETQMGGYMSYGQSLGYLTMVALFFSLVMTIWTPVFMKVIAPDFMAQQME
                     LARQRMEAKGLTQDQIEMGMKFSRKFMSVPFMMIFALFGNMLWLTIINLIVSAFMKKD
                     KPVDFNSDPNLPPPPAPF"
     gene            9795..10763
                     /locus_tag="VFU15_13175"
     CDS             9795..10763
                     /locus_tag="VFU15_13175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005467684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU4718786.1"
                     /translation="MDISVVIPLYNEAESLPELHDWIVRVMQANHFSYEIVFIDDGSR
                     DSSWKVVEQLAEKNPNVRGIKFRRNYGKAAGLNVGFEAAHGNVIITMDADLQDSPDEI
                     PGLYGMIVKEGYDLVSGWKKKRYDNAMTKNLPSKLYNATTRWMSGVKLHDMNCGLKAY
                     RSDVVKSIEVYGEMHRYIPVIAARAGFTRIGEKVVQHQARKYGESKFGASRFVRGPLD
                     LLSIMFVSKFGKRPMHIFGLIGTLMFMLGFFSAVYLGVSKLWIVYHDHAVAPRVTESP
                     WFFIALTAMVIGTQLFLAGFLAELIARNSPDRNVYLVEKRIGEGVA"
     gene            complement(10780..11739)
                     /locus_tag="VFU15_13180"
     CDS             complement(10780..11739)
                     /locus_tag="VFU15_13180"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEU4718787.1"
                     /translation="MEKKTSRNLRLGFFVLLGTLFLVLGLYMMGNQKNLFGSTFHLKA
                     YFKNVNGLMEGNEVRLFGINVGTVESVEIKSDSAVEVVMLIKEGTEKYIKKNCLAKVG
                     TDGLMGSKIVNLQNPPQGATIVTDGDIISVDNGINTDEVMRTLNATNENIKTITDDLK
                     DITGKLRGQNSLWKLLGDSVLAQNLNSAIVNIKLTGQNTAIISGDLSTIIRNVKNGKG
                     TIGALVTDTSLATQVKQSVVNIDFISDRLAVVSGNLDVLTTTAKNGQGSVGQLIMDTT
                     FVHNLNQGILTVNKGAQGFSDNMEALKGTWPLRRYYKRQAKGK"
     gene            complement(11743..12519)
                     /locus_tag="VFU15_13185"
     CDS             complement(11743..12519)
                     /locus_tag="VFU15_13185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU4718788.1"
                     /translation="MTELKERTEKQERVPQNGAIISIAGLNKAFGENKVLMDFNMELF
                     PRENVVILGKSGAGKSVLIKCIIGLMLPDAGSIKVFGSEITKMKTSDLDKMRIRVGFL
                     FQSNALYDSMTVRENLEFPLRRHGTDKSKKEIADMLHEALSQVGLAHTLDMMPSELSG
                     GMRKRIALARTLILKPEIILYDEPTTGLDPITGRGISQLILDIKERYGTASIIISHDM
                     NCVRHTADRIIVLIDGRCYAMGTYEELKRSDDSKVKQFFE"
     gene            complement(12521..13288)
                     /locus_tag="VFU15_13190"
     CDS             complement(12521..13288)
                     /locus_tag="VFU15_13190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010855074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU4718789.1"
                     /translation="MKNDVLRNFLLETADLSRFAWRFFREALRPRYEVGEFLRQCFFV
                     GYKSLGLVGITAFIMGLVITIQSRPTLVEFGAEAWLPNMVSVSLVREIAPVITALICA
                     GKIGSGMGAELGSMRVTEQIDAMEVSGTNPFKYLVVTRVFATTFMVPLLAIMADAISL
                     FGAYIGANIRGIVSWQLFWAKTWDSVFFSDFFPAFIKTFFFGMAIGIVGCFKGYSTSG
                     GTEGVGKSANAAVVIASLLVFIIDLIAVRITDILNLN"
     gene            complement(13376..13627)
                     /locus_tag="VFU15_13195"
     CDS             complement(13376..13627)
                     /locus_tag="VFU15_13195"
                     /inference="COORDINATES: protein motif:HMM:NF020967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HEU4718790.1"
                     /translation="MPASIFVTVATFTYVHEAVIPRAKLESHGILCFIRDEFSMYMQP
                     FFSFSGGGIKLQVHADDEADAVKILSEAGYFRGDRTQWQ"
     gene            complement(13870..>14095)
                     /locus_tag="VFU15_13200"
     CDS             complement(13870..>14095)
                     /locus_tag="VFU15_13200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013927378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEU4718791.1"
                     /translation="PVLMTALVAALALIPLTLDSQAAGKEILYPVATVILGGLISSTL
                     LDMIVTPVVFYKFGERALKHYFDNKAKQDL"
BASE COUNT         3295 a         3610 c         3791 g         3399 t
ORIGIN      
        1 aaacatttct cggatccgga acgacgatcg tcaatgaaaa aaaggggatc gagcaatggg
       61 ccggtgcagg ctggacaggt cagccgctga tcgtggacga gtgcggaaaa aaatttctca
      121 tccagggttg cttcgatcat cacctgaaaa agatcgatgc gcagaccggc gatgtgaagt
      181 gggaatatgc ttatgacgat atcctgaaag gaaccggcac gatctggaag aacgacagcg
      241 cagccgatcc ggaaaaccgc atcgagatca tacagggcag ccgcaagggc gtgaattccc
      301 cgctttgcgg cgcgcacgct tacagcttcc gcgcggtttc gtatttcacc gggaaagagc
      361 tctggcgcat ggatgtggag cacaccgcca gctacagccg cgactgtgat gcttcggccc
      421 tcgttatttc cgatacggcg tacatcggtc tcgagaacgg gaaattcaaa tggttcgacc
      481 cgggaaagaa aaccatacca tccgattcgt tcgcggctcc cgacgttttc ggctgcacgg
      541 atctttacaa ccgggaagat gctgcgcatc acggtggcaa cctcgtaacc gaaagttctc
      601 ctgcgcacat cggcaatcac atttacatca cggccggaag tggtcacgtg tatggtttca
      661 atctcgccac aaaaatcatc gactgggatt ttttcatcgg ttcggacatg gacggttctc
      721 ccgtagtgac atccgacagc tgcctgctca tcccggtgga aaagcaatac atcgcgggca
      781 atggcggcgt gttcaagctc gatccgtcga agtcgccgga caactgcgtg caatggtatt
      841 ttccgtccgc cgatttttct tttgcttcgt gggaaggcgg ggtcatcggc agtgtggcgg
      901 tgaacgaccg gtacaacgac ggtgaattcc cgcgtcttgc ttccttcatg ggaattgacg
      961 gttatctcac ggtcgtgaaa tacgatcatg tgcggaaaga cacgcttgtt aaaggtccgg
     1021 acggaaaaac cgtttatcca tgcccggaag tggtgttccg gaaacggaca gggccttcga
     1081 tttcgacgcc gctgtttgta aatgacaagc tcgttgcggc ggggtattcc gggatctgtc
     1141 ttttccgtta cgaccgcggc ggtttttttt atgagctcgc tttccacccg ggaatttttg
     1201 aggcgacgcc gtctgccgat gcaggaaaaa ttttcattgc gtcgcgtgac gggttccttt
     1261 actgcttcgg tgacgcggga acgggaaccg ggaaagaagc gctatcgcac aacactcctg
     1321 caccggtaac gcaggctgtc gccgcaaaga aaaagaagga cgcaccgcct gccgtgcaga
     1381 agaaagaaga aagaaatacg ttccatctcg tggcgggtgc attccgcgta aagcagaacg
     1441 ccgtcaattt caaaaaacag tgggaagcga aaggggtgag cggctcgctg atcgaggcgc
     1501 cgatggacct gtattacgtt tcattgttcg atacggaatc caaagaagcg gccgaccgta
     1561 aacacgatga gctcatgaag cagttcgggg cggatacgtg gatctacacg ggaaattaat
     1621 ccagctccgg ctggttgttg tagagatcgc gcaggtaagc cggcgttcct ttctgcatgt
     1681 cgcggaacaa accggaactt ttcacttttt ccagcttgtc atcttcgatg aaaacgatga
     1741 tcgcgatctt gtcgcgcgtt agcaccgtat agaatgcgcc cccggctttc tgctcttcca
     1801 tcgctttgtt gacgtcgtcg atcaggcctg tgcagagcac ggtgccgcgc tgcatttcct
     1861 ccgattcata tactttcccg ttgagcgaga aacggacggt gtgatcagtg aaattggcat
     1921 ctgttgtcgt atccgaaaca atgccgggat tgaaatgccc gcgtgaaatg tgcgtcatcg
     1981 ctttcagcat ttcagcgtaa ggattgtaat cattgatcgt atcgaagctc accgtggtga
     2041 acaacgtatc gatgtaatcg taaaaggctt cgcttccgta aatggtgttt tctcttatct
     2101 cttctttttt cgcatcatac cagggtttgt ctttcgccgt cacgagtccc gatgattcca
     2161 gccgcgtgaa catgctgtcg agctggtgcg ggctcatcgt tgaaaattcc tcttcgggat
     2221 cgagcgcaag gtcccacatc ggcatgagcg aatcttcctg cgctttggtg agcatgatca
     2281 ggccgatggt gttgatgtcg aacttcacgt tctcgtcgtc ccaggggttg acacaattcg
     2341 tgcagcggta cgagatcatg aacatgcggt agctcttgtt ctgctgggcc agcatggtgt
     2401 tgtagacctt gcagaacgtt tcatcgatag gccagtcgcg gaaccagtag agcttctgca
     2461 ggaaaacgtc gctgcctgct ttcatccgca tgagcaccca gtcggtgtcg tgatcttcgg
     2521 gatagtccgc gagataatga taacgggtgg aatcaacgcg cagctccggg tatcttttcc
     2581 cgatttcatt gcagaggcgg tcgtacactt catgcggcgc cttcccgttc tcgcggagat
     2641 cgagcttcat cgcttcgcca ctgtaaacga gcaggtcctt cagcccgttc agcgtgtctt
     2701 tcgcataaac agggatgagc gaatccaggg ttttgtccgg gatattattg agcaatccca
     2761 cgtgacgaag cgtgagcacg tctttgcgga aacgatcggt gaactggtcg ttcttcgtgc
     2821 tgccggactc gctttttttc gggttgttcc cgcaggaaag gaaaaatact gcgcaggaaa
     2881 gcagcgtaag gatccggagc atacgttcag ttgatttggt cttcgggaag cggtggccgc
     2941 gtcgatttgt atttttcaaa ggcgtcgagc atatcgcccc atgaaaaatg tttcggatcg
     3001 ggaacgggat aggtaacggc aaacccggag aacatatccc attcaccctc ggtgaaaccg
     3061 agctgcccga gcaaaggctt catgcggtaa atgtcttttt tctggaacgg gatcgtcttc
     3121 aggctgtcga gcgtcgccac tttcaggtga agcttgtagt aggaatccgt ggtgcggaat
     3181 ttttcaaggt gctcgataaa atcgccgtac gtgacggtgt cgcccaccac gcgacgttcg
     3241 ctcagccacg cgggaaaaag attcggaacg acgtacaggt aatcgaagct gtcgatcccg
     3301 cagcgcgaaa gcccgataag gtctttatcg atcgagtcct gcggataatt tttaggaata
     3361 cgttttttgc ggatcgcgca ttcgtccttc ggggcagtgt cgcttacggc aacttttccg
     3421 gtgtcggtaa ccgatgaatc gcccgggacc ttcgtgctct tgtcgttgga gcaggaggca
     3481 agcgcgaaaa gcaggaatgc ggagaggatg gtcttttgca cgggtttcaa atttacagaa
     3541 atgatgcgtt acgggaataa gccgtcgtgc caataaaagt tacatcgccg tagttccctt
     3601 tttcagcccg ataaggaata ccgccgaaac gacgttgctg ttcacataag gaaaatattt
     3661 tcccgtaagc cgcaggttat agccataaac gaccttgaac ggccccatgc cgatgccgat
     3721 ctcgggtcgc aggcgcaatg atcccgcgct gaaatcagta tagtaaatgg catttatacc
     3781 cagcgccatg gcggatgcgc ctcccgctat ccatccgccg atcttcggtc ccatcacaaa
     3841 gtgatgatca tccattttca gctcgtcgga aatgaatacg gcgctggcaa acgggtgaac
     3901 gccggcaagg gtaaaactgt tcttcgccac gccgacctcg ccgtatgtcc agtgccagta
     3961 gttgatgcct aaaaggagag aaatatcctt ttccatgggc atcagcgacc gggaaaattt
     4021 cgctgtgtcc gcctgtccgg cgaggaaaaa cgggagaagg atgaatacgg agaccaggat
     4081 ttttttcagc atggcgcagg gacggcagtt ttataacaca gaaattcgcc gaagatacac
     4141 gccacggaaa aatcaccagc ccatgatgtc gatcattccc cagagcagca tcaccacacc
     4201 ggcgatcgtg accgaataca tggcgtaatt gatattgctg ccgctgtgat aaagcaggag
     4261 ggtgataaga aacccgaaca caaggaggaa agagccgatc gtcacgctgc ggaagccccg
     4321 tttttttcgc cggatgaagt cgatcgtttt gatctctttc atgatatggg cggtcatctc
     4381 cggcgttgcg cccgctttct gaagattttc ttcgatgtga tgattttcaa ctccgcgtgc
     4441 atgctggtct gaggcgagca gacgcagttc ctggtgtttt tgctggtcca tgtgctttct
     4501 gaggcaagct tttcctgcag cgggaaggca ggaaactgac ggatcccaat gatgaaaaat
     4561 acaggattgc gatggcaaaa gcaaatcgcc cgatgcaaaa cggatttatt ggttaatttc
     4621 agcgcacaaa aattctctta tggaaatcaa gcatactgtt ctcgagcggt tcctgaaata
     4681 cgtccaggtg gatactcagt cggacccgga ttctcccacc tgcccgtcaa cggaaaagca
     4741 gaaagatctc gggaaaatac ttgtggaaga attgctcgcg atgggcgtga aggacgcgca
     4801 catggatgcc aacgggtacg tttacgcgac gattccctcg aacacgaaaa acaaagttcc
     4861 tgcgatctgt ttttgttcgc acatggatac gtccccggat tgttcgggga aaaacgtaaa
     4921 acctgtcgtg cacaaaaatt acgacgggaa agacatcgtg ctcccggctg ataaaagcca
     4981 ggtgatccgc ttcgcggaac atgaagcgct ggccggccag ctcgggaacg atatcgtcac
     5041 ggccgatgga acgacgctgc tcggcgcgga caataaagcc ggtcttgccg agatcatgga
     5101 cgccgcgtat tatttcatga cacaccccga tgtcaggcat ggtgatatta aaatactttt
     5161 cactccggac gaagagatcg gccgcggggt ggataaagcc gacctgaaaa agctgggcgc
     5221 tgaattcggt tacacgatgg acggcgaaac cgtcggccat ctcgaagatg aaacgttttc
     5281 cgccgatggt gcatcgctta ttattcacgg cgtgagcaca catcccggtt ttgcaaaagg
     5341 aaagatggaa aacgcattga agatcgccgg gcgcattctt gccgaacttc cgcgcacaac
     5401 ctgtacgccg gaaacgacgg aaaagatgga aggttttatt cacccgactt cagtgaacgg
     5461 aacgctcgag caggcaacga tcaatttcat tctccgcgat ttttcggaag aaggcctgcg
     5521 cgacaaagag aaaacgcttt catcggtgat ccgcacggtg atgaaagatt acccgaaatc
     5581 gacttaccag cttgtcgtga aggagcaata ccgcaacatg aaagaagtgc tggacaagca
     5641 tccgcgcgtg gtggattatg cgctggaagc cattcgcagg acgggaatcg agccggtgcg
     5701 atcgagcata cgcggcggaa cggacggaag ccgcctttct ttcatgggat tgccctgccc
     5761 gaatattttt gccggggagc acgcattcca ttcgaagcag gaatgggttt ccgtgcagga
     5821 catggaaaaa gccgtgcagg tgatcattca cctggtgatg atctgggaag agaaaggctg
     5881 atgacgttat tgataaaaga aaacggcggg aaaaattttc accgccgttt ttttatggag
     5941 ggaatttgcc tcagctgaat tttttcgcgt cggcgagaaa ttgggcgaga ccattatccg
     6001 taagcggatg tttcagcagc gccatgatcg cgctgagcgg gcaggtggcg acgtctgcgc
     6061 cgatcttcgc acagtccacg atatgcatgg gatgacggat ggaagcggcg aggatctgcg
     6121 ttgcaaatcc atagttgtcg tagatctcgc gtatttccgc aatgaggttc agcccgtcgg
     6181 tggaaacatc gtccagccgg ccgataaaag gagaaacata cgcagcgccg gctttcgccg
     6241 cgagcagcgc ctgcccgggc gagaagacga gcgtgcagtt cgtgcggatt cctttcgaag
     6301 aaaaatatct gatcgctttc acgccctctt tgatcatcgg cactttcacg acgatctgct
     6361 tgtgcagctt cgcgagcttt tcgccttctc tcacgatgcc gtcgaaatcc gtggaaatga
     6421 cttccgcgct gacgtcaccc gtaacgatgt tgcagatgtc gacgtaatgt ttcatcacgg
     6481 cagcctcgcc ggaaatcccg actttggcca tgagcgaagg gttcgtggtg actccgtcca
     6541 gcacgcccag ttcctgggct tctttgatct gtgcgaggtc ggcggtatcg atgaagaatt
     6601 tcataatgcg ctgcgttttt atcgctgcaa atttacggga atgccggctt tggttattaa
     6661 caaattatac aatcactgcg aagctttctt cggcgggtaa aacatcagtt cacaataagc
     6721 ttgcgctgaa cggaagcgcc ggatgatgtt gtaacctgca ggaaataaat tcccgggctg
     6781 agcgcattca ccggaagatc gtacgacgtg ccattcaccg cgatattttc cgcaactctt
     6841 cctgaaaggt caatgacgca gagtgatttc atcgggtcat ggccggaaga aatatgtacc
     6901 acgtcatcag acgggttggg ataaatggaa aaaacatttt catctccggt tttttttatc
     6961 ccggtgaggt cgcacgtgta cggataagga ttcagctcga tgggttgtcc tgcggaaagc
     7021 gctaccgaat tgaagccgct gtacgacgaa taatagaacg ggctgcggaa aacgagattc
     7081 ccggtgggcg tgctgccctg tgaaagaatt ccggttcccg aaaccgggtt gatgtatttc
     7141 cacaccgtat gcatcgtgct gtcaatctcg aaaaaggttc cggccggccc gttgcagatc
     7201 aggacgtccc cgttggccag ttgctgcgcg ccggaaatat tggtggcgta aaaattcgcc
     7261 ggcacgctgt cggcatagat ccagtcctgc gccgcaggca ggtagggcag gctggatgaa
     7321 taatttcccg cagaatcgac cgggggagaa atgatctcga ccgtggaata attcccggct
     7381 ggtcgcccgc ttccgttatt gaaaaccatg atctcgtttt catgcggaag tcccggtgtg
     7441 atccagtgcg cattgtgctg gccgaagaac atcttgttcg atgcgttgcc gtggttgtac
     7501 gcggcaggat ttccccagcg ccagaggatg tcgccgccct tgccggaatt tccgccggtg
     7561 tggccggccg cttcggctgt tgttgtgctg tgatcgatca cccagatctc gttcaggttg
     7621 tgtacgctca ctacgatctg gtcgagggcc gggttgtagt cgatcgaatt gaaatgcgcc
     7681 cagtcggctg ctgtgcttcc gctgtaattg atgttcacca gctgcgggtt gttgccaacg
     7741 atcccgtaat tcgcttttgt gctgtcgtaa tgttggatga gatggtccca caggtgccat
     7801 tcccagacaa tgttcgcgga attgttcccc accggctgca gctccacgat cttttcgccc
     7861 cacagctccg atccgaggaa ggcaggattt cttcccgcgg cgatcgcttc ggcctgtgtt
     7921 tttttctccc acgcgatgac gagaatgttc ccgtccggca gcgcgcatat atcgtggtgc
     7981 tggcattgcg aagtgtcgga aacggcatag ctccagagca cattgctgtt ccagtcgagc
     8041 ttttcaatga ttcctcccgc accgccggcg acaaaatcag ggttgttcgt aattccggcg
     8101 cggagaagcg ttccgtccgc ctggaaatag gcggattgcc cggggcggta ggcgctcgtc
     8161 cacgaatgaa tttccttgcc gcatttatca atgagataag tggtcgtatc cgatatcggt
     8221 gcaaaaagca cataaccgcc gtcttcgcca ccggtgtgct ggataagtcc gacggtcggc
     8281 gtttgcgaat gaacgaaagg gcataggaga agcaggagtc cgggtaatgt aattttcatc
     8341 agttcgtttt ttgtgttgga cggcgaatag ctttttttct ttcgcgcgat accggtgaag
     8401 ggaaaaagaa aatcggtgtt ttatcccggg gagcttgttt tttcccacac ttcgcagtcc
     8461 atcagccggc cttccagcgt ccggaaatca ttccgcagcg ttccggaaaa tgtgtagccg
     8521 gctttttcag cgactttccg cgaagcggaa ttccggtttt cgatacggca catgagcttt
     8581 gcgaatttct gctcaccgag aaaatagtcc gttacttttt tcagtgcctc ggaagcgaaa
     8641 ccgttcccgt aaaattcctt cgcgaggaaa tacccggcct cgcccttcgg cacgcgttga
     8701 ttgatgtcct tgacgatgat ctgcccgatg agcttttcct cctttcgcag gaacacgccg
     8761 gagaaaatgg atatggccgc cgaccgttcc cggttgcgga ggagaatata tttccgcgcg
     8821 gccatgaccg aagaaaggtt ttccaccacg ttcgggaaat attctttcag ctcttcccgg
     8881 ttgccgttca ccagtgaaaa aagatcggcc gcatccgtga tccggtagtt gcggacgagg
     8941 agccgttcag tttccaggaa taggggagga atgttcatgc aggtcatgca aaatcattcg
     9001 ccggagctca gggaatgaaa taaaaaatgg gtaagcgact tatatcgcac cgctacaacc
     9061 acctaccctt gcttccttcc ggacctgggg gattcagcag gagctggtcg tataagactt
     9121 acccgcgcca aagataccac aatctgtttc aaaatctaca acaaaaaata aaccgggatc
     9181 ccaaagcctg ttaacccaag gcaactcccg tggcattctg ctatatttgc ggcgaacaaa
     9241 tcttcacagt catgctaaaa cactctctga tatacggggt ggtcatttcg tttgcagtga
     9301 tcgctcttgc gctcgggatc tacctttccg gcgtaacaaa ttccgccacg aactacctgt
     9361 cgtacctcat ttacatgggg atcatcattt atgcggcgaa aaactaccgg gaaacacaaa
     9421 tgggtggata catgagctac ggccagtcgc tgggctacct cacgatggta gcgctgttct
     9481 tcagccttgt gatgacgatc tggacgccgg tctttatgaa agtaattgct cctgatttca
     9541 tggcccagca aatggaactt gcgcggcaga gaatggaagc aaagggcctc acgcaggacc
     9601 agatcgaaat gggcatgaaa ttctcccgga aatttatgtc ggtgccgttc atgatgattt
     9661 tcgcactgtt cgggaatatg ctctggctga cgatcatcaa tctcattgtt tccgctttca
     9721 tgaagaagga caaaccggtg gattttaatt ccgatccgaa ccttcctcct ccgccggccc
     9781 cattctgatc attcatggat atttccgtag taatcccgct ttacaacgaa gccgaatcgc
     9841 tgcctgagct tcacgactgg atcgtgcgcg tcatgcaggc gaatcatttt tcctatgaga
     9901 tcgttttcat cgacgacgga agcagggaca gctcgtggaa agtggtggaa cagctggcgg
     9961 aaaaaaatcc gaatgtgcgc ggcatcaaat tccgcaggaa ctatggcaaa gcggccgggc
    10021 tcaacgtcgg tttcgaagcg gcgcatggaa acgtcatcat aacgatggat gctgatctgc
    10081 aggacagccc ggacgaaatc ccgggtcttt acgggatgat cgtgaaggaa ggctacgacc
    10141 tcgtttccgg ctggaagaag aaacggtacg acaacgcgat gacgaaaaat cttccgtcaa
    10201 agctgtacaa tgccacaaca cggtggatga gcggcgtgaa gctgcacgac atgaactgcg
    10261 gactcaaagc ttaccgcagc gacgtggtga aaagcattga agtgtacggt gaaatgcacc
    10321 gctacattcc cgtcatcgcc gcacgtgcag gtttcacgag aataggcgaa aaggtggtgc
    10381 agcaccaggc gcgcaagtac ggcgaatcga aattcggtgc gagccgtttc gttcgcgggc
    10441 cgctcgacct gttgtcgatc atgttcgttt cgaaattcgg caagcggccg atgcacatct
    10501 tcgggctcat cggcacgctg atgttcatgc tcgggttttt ctcagccgtt tatctcggcg
    10561 tcagcaagct gtggatcgtg taccatgatc atgccgttgc accgcgcgta acggaaagtc
    10621 cgtggttttt cattgcgctc accgcgatgg tcatcggaac gcaattattc ctcgccggtt
    10681 ttctcgctga gctgatcgcg cgcaattccc ccgaccgcaa cgtttacctt gttgaaaaaa
    10741 ggatagggga aggcgtagcc tgataattat gatgattaat catttgcctt tagcctgccg
    10801 cttgtaatac ctccgcagcg gccaggttcc tttcagcgct tccatattat cggaaaatcc
    10861 ctgcgcacct ttattcactg tcagaattcc ctggttgagg ttgtgcacga aggtggtgtc
    10921 catgatcagc tgccccacgc ttccctgccc gtttttcgcc gtggtggtga gcacgtcgag
    10981 atttcccgag actactgcaa gccggtcgct gatgaaatcg atattcacaa ccgactgttt
    11041 cacctgcgtg gcgagcgatg tatccgtaac cagcgcaccg atcgttcctt ttccgttctt
    11101 cacattgcgg atgatggtgc tgaggtcgcc ggaaataatg gctgtattct gtcccgtgag
    11161 ttttatattg acgatggcgc tgttcaggtt ctgggccagc acggaatcgc caagcagctt
    11221 ccagagtgaa ttctgtccgc gcagcttgcc ggtaatatcc ttcagatcgt ccgtgatcgt
    11281 tttgatgttc tcgttcgttg cgttgagcgt gcgcatcact tcatcggtgt tgatgccgtt
    11341 gtcgacggag ataatgtcgc cgtccgtcac gatcgtcgcg ccttgcggcg ggttctgcag
    11401 gttaacgatc ttgctgccca tcagtccgtc ggttcccact ttggcgaggc aatttttctt
    11461 gatgtatttt tccgttcctt ctttgatcag catgaccact tccaccgcgg aatccgattt
    11521 tatttccact gattccaccg tgcccacgtt gatgccgaac agccgcacct cgttcccttc
    11581 catcaggccg ttcacgtttt tgaaatacgc tttgagatgg aacgtggagc cgaaaagatt
    11641 tttctggttg cccatcatgt aaagccccag taccaggaac agggttccca acagtacaaa
    11701 aaagccgaga cgcaggtttc gcgatgtttt cttttccata atctattcga aaaactgttt
    11761 cactttgcta tcgtctgaac gttttaattc ctcgtacgtt cccattgcgt agcaccgtcc
    11821 gtcaatgaga acgatgatgc ggtctgctgt gtggcgaacg caattcatgt catgggagat
    11881 gatgatcgac gctgttccgt agcgttcctt gatatcgaga ataagctggc tgatcccgcg
    11941 cccggtgatg ggatcgaggc ccgtggtcgg ttcatcgtac aggatgatct ccggcttgag
    12001 aataagcgtg cgtgcgagcg cgatgcgttt gcgcataccg cccgaaagtt ctgaaggcat
    12061 catgtcgagc gtgtgcgcaa gtcctacctg cgaaagcgct tcgtgcagca tatcggcgat
    12121 ctcttttttc gatttgtcgg tgccgtgcct gcgcaaaggg aattcgagat tttcacgtac
    12181 cgtcatcgaa tcgtaaagcg cgttgctctg gaaaaggaag ccgacgcgta tgcgcatctt
    12241 gtcgagatcg gaagttttca ttttcgtgat ctccgagccg aaaactttta tcgatcccgc
    12301 gtccggcagc atcaggccga tgatgcattt gatgagcacc gatttcccgg ctccggattt
    12361 cccgagtatc accacgtttt cgcgcggaaa aagctccatg ttgaaatcca tcagcacttt
    12421 attctcgccg aaagctttgt tcaggcccgc aatgctgatg atcgcgccgt tttgcggcac
    12481 acgttcctgt ttttccgttc tttctttcag ctccgtcatt tcagttcagg ttgagaatat
    12541 cggtgatccg cactgcgata agatcgatga tgaacaccag cagcgatgcg atcaccactg
    12601 cggcgttggc agactttccc acgccttccg ttccgcccga ggtggaatat cctttgaagc
    12661 acccgacgat gccgatcgcc attccgaaga aaaacgtttt gatgaaggcc gggaaaaagt
    12721 cggaaaagaa aacggaatcc cacgttttgg cccagaataa ttgccagctg acaatgccgc
    12781 gtatgttggc gccgatatac gctccgaaaa gcgagatcgc atcggccatg atggccagca
    12841 ggggaaccat gaacgtagtg gcgaaaacac gtgtgacgac aagatatttg aacgggttgg
    12901 tgccggaaac ttccatcgcg tcgatctgct cggtcacgcg catggaaccc agttcggcgc
    12961 ccatgcccga cccgatcttg cctgcgcaga tgagcgcggt gatcacggga gcgatctcgc
    13021 gcacaagcga aacggaaacc atgttcggca gccaggcttc ggccccgaac tccaccagcg
    13081 tgggccgcga ctggatcgtg atcacgagtc ccatgatgaa cgcggtgatg cccacgagcc
    13141 cgagcgattt gtatcccacg aaaaagcact ggcgcagaaa ttccccgacc tcgtaacgcg
    13201 gacgtaaagc ttcacggaaa aaacgccatg cgaacctgga cagatctgcc gtttcaagca
    13261 aaaaatttct cagcacatcg ttcttcatag gatttttttc tttcgcaagt tggcaacgat
    13321 ctcttcagcc gggtgaagcc ggggtcaccg cccgccctga cagttgtcat aaaacttatt
    13381 gccactgtgt ccggtcgccc cggaaatacc cggcctccga caatattttt accgcgtccg
    13441 cttcgtcgtc ggcatgcacc tgcagcttta ttcctccacc tgaaaatgaa aagaaaggtt
    13501 gcatatacat cgagaattcg tcgcggatga agcacaggat gccgtgcgat tccagctttg
    13561 cgcggggaat aacggcctcg tggacatagg tgaacgtcgc aaccgtgacg aaaatagaag
    13621 cgggcatggc taaaaatagg aaaaacaccc attcgctgaa acccttgtgc cacttggact
    13681 ggcagggaat ttcgtatttc gagagtaacg gaatagaacc aaaaaccaaa tattgacgtt
    13741 accgggggcg attttcacct ttgacaggta attagtttcc gatgttatca aaaaccattg
    13801 accgataccg ttacaaagaa ctatcgcatc taagataatg caaattcaac aaactacatc
    13861 agattccatt tataaatcct gctttgcttt attgtcgaaa taatgcttta aagcccgttc
    13921 tccgaatttg taaaacacta ccggcgttac aatcatatcc agcaatgttg agctgataag
    13981 tccgccaagt atgaccgttg ccactgggta aagaatttct tttccagcgg cctgcgaatc
    14041 gagcgtgagt ggtatcaggg caagtgcagc caccaatgcc gtcatcaaaa caggg
//
LOCUS       DASTSF010000453         2029 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1220402,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000453 DASTSF010000000
VERSION     DASTSF010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1220402"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..380
                     /locus_tag="VFU15_13205"
     CDS             <1..380
                     /locus_tag="VFU15_13205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HEU4718792.1"
                     /translation="GHAVLRKTDPRFTAQQDFYNRYIKNEKGVDSLCEIVQMIYKVAP
                     PILEGTGKIKNPWPNVDAHSGALLMHFGITEFDFYTVLFGVSRALGVLASLIWDRALG
                     HPIERPGSITTAGIKKKIGLVQA"
     gene            complement(538..1062)
                     /locus_tag="VFU15_13210"
     CDS             complement(538..1062)
                     /locus_tag="VFU15_13210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018781873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU4718793.1"
                     /translation="MLVPGKDYHIRPLSEKDDLEELTRLLNKSYGKLAERGFRYLASH
                     QDAEMTKQRIQNGHCLVAVKGDRIIGTICYYSPGNKSYGKWFHSANAASWGQFAVDLS
                     LQGNGIGNNLILAAEDLARTDRAEEILIDTAEGAHDLIAFYGERDYHFVAWAQWDETN
                     YRSAVLRKKIHYST"
     gene            complement(1077..>2029)
                     /locus_tag="VFU15_13215"
     CDS             complement(1077..>2029)
                     /locus_tag="VFU15_13215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEU4718794.1"
                     /translation="SAVTEVSGDAHDSLLFFTLATFTAPPTIFKYNLGTKQTTEYFKP
                     QIDFKSDEYETEQVFCTSKDGTKVPIFITHKKGIKMDGNNPCFLFGYGGFDLHYAPEF
                     RTDRAVFLEAGGVYAVANMRGGDEYGEKWHLAGTKCNKQNVFDDFIAAAQYLIDNKYT
                     NSQKLAVHGRSNGGLLIGAVETERPDLFKVCIPMVGVLDMLRYQQFTIGKAWSSDYGL
                     SSNEEEFHCLYKYSPLHNVKRTSYPATLITTGDHDDRVVPAHSFKFAATMQENQLGDN
                     PVLIRIDHNAGHGGGKPTEKQIEEYTDMWSFVFFNLGMNY"
BASE COUNT          439 a          510 c          569 g          511 t
ORIGIN      
        1 atggtcacgc cgtgctgcgc aagactgatc cgcgtttcac tgcgcaacag gatttttaca
       61 accgttacat caagaatgaa aaaggagtgg attcgctttg cgagatcgtc cagatgatct
      121 acaaagtggc gccgcctatc ctcgaaggaa cggggaagat caagaacccg tggccgaacg
      181 tggatgcgca cagcggagcg ctgctgatgc atttcggaat tacagaattc gatttctata
      241 cggtgctgtt cggcgtttcg cgtgcgctgg gcgtgcttgc ctctctcatc tgggatcgtg
      301 cgttgggtca ccccatcgag cgcccgggtt ccattacgac agccggtatc aagaaaaaga
      361 tcgggctggt gcaggcgtaa atgcaacgat gaaatataaa agaaaccgct tcgtgagagg
      421 cggttttttt tgtgggtggt ctcgacttgc gctcgacctg acaagggggc gacttgcgct
      481 cgaccggaca agaggtctcg actatcgctc gaccggacaa ggaggaccgg aattgtttca
      541 ggtggaatag tggatctttt tgcgcaggac agcgctgcgg taattggttt catcccattg
      601 tgcccaggca acaaaatgat aatctctttc gccgtagaac gcgataagat cgtgcgcacc
      661 ttctgccgtg tcgatgagga tttcctccgc gcggtcggtg cgcgcaagat cttctgcggc
      721 gagaatcagg ttgttgccga tgccatttcc ctgcagggaa agatcgactg caaattgtcc
      781 ccaacttgcg gcgttggccg aatgaaacca cttgccgtac gatttatttc ccggcgaata
      841 gtagcagatc gtcccgatga tccggtcgcc tttgacggca acaagacaat gtccgttctg
      901 aatccgctgc ttcgtcattt ccgcatcctg gtgggaagcg agatacctga agccgcgttc
      961 agcgagtttg ccgtacgatt tgttcagcag gcgggtaagc tcttcaagat cgtctttttc
     1021 cgagagcgga cggatgtggt aatcttttcc gggcaccagc atcgcacgca gcgggatcag
     1081 taattcatgc cgaggttgaa gaacacgaac gaccacatgt cggtgtattc ttcgatctgt
     1141 ttttcggtgg gttttccgcc accatgccct gcgttgtgat cgatgcggat aagaacaggg
     1201 ttatcgccga gctggttttc ctgcatcgtt gcggcgaact tgaacgagtg cgcgggaacg
     1261 acacggtcgt cgtgatcgcc ggtggtgatg agcgtagcag gatacgatgt acgttttaca
     1321 ttgtgcagcg gggaatattt atacaggcaa tgaaattctt cttcattgct gctgagcccg
     1381 taatccgacg accaggcttt cccgatcgtg aactgctggt agcgcagcat gtcgagcacg
     1441 ccgaccatgg gaatgcatac tttgaaaaga tccgggcgct ccgtttccac cgcgccgatg
     1501 agtagcccgc cgttggaacg tccgtgcacg gcaagcttct ggctgttcgt gtatttatta
     1561 tcgatcaggt attgcgcagc ggcaatgaaa tcgtcgaaca cattctgctt gttgcatttt
     1621 gttccggcga ggtgccattt ttccccgtat tcatctccgc cgcgcatgtt ggcgaccgcg
     1681 taaacaccac ccgcttcgag gaagaccgcg cgatcggtcc tgaattcggg agcgtaatgc
     1741 aggtcgaatc cgccatagcc gaaaaggaag cagggattgt tcccgtccat cttaattcct
     1801 tttttatgcg tgatgaaaat gggaactttc gtaccgtcct tgctcgtgca gaacacctgc
     1861 tccgtttcat actcatcgga tttgaaatcg atctgcggct tgaaatattc cgtcgtttgt
     1921 tttgttccga gattgtattt gaaaatagtg ggcggagccg taaatgtggc gagcgtgaag
     1981 aacagtaaac tgtcatgtgc gtcaccgctg acctctgtca ccgccgaca
//
LOCUS       DASTSF010000454         4708 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1243186,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000454 DASTSF010000000
VERSION     DASTSF010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4708
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1243186"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            141..803
                     /locus_tag="VFU15_13220"
     CDS             141..803
                     /locus_tag="VFU15_13220"
                     /inference="COORDINATES: protein motif:HMM:NF014628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HEU4718795.1"
                     /translation="MDESTSREKILKKVRAALINKSRQEPSPVDLESNVFESPSDSIE
                     LVFAQQFSDAGGQFVFCENEEEFAYNIGALIADGSFGKIWCAEKECQELLSQAGLKFL
                     SKEKDIASAQTSLTGCEFLIARTGSALVSSRQASGRKGHVFPDSHIIVAKTSQLVMHV
                     KDALREMRNRYPSGLPGMLTMITGPSRTADIEKTLVQGVHGPKEIYVFLIDDVPQENV
                     QE"
     gene            814..1722
                     /locus_tag="VFU15_13225"
     CDS             814..1722
                     /locus_tag="VFU15_13225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718796.1"
                     /translation="MKKFLVPLLLAISVPVLAQDSTRVPFFKPEAIGFYSGITAFRDV
                     PVTISDLQKIAPSSALLGADLDSFKTNTFTGSGNVNSVFGMGVDFRYRMRSNGQYAGF
                     SRIRASIMYTNGQTAYREFYRNSTVRTDTVRLSNGQQFYVDSIHNEYYTYSWNYQALG
                     IDLNQVFHTKDARIFSAYTGYGIHFGLIIGSYFSATHELQTGSQFLTTDSTATGSSMG
                     YFTTNTRQDETENIATKNYYTFSFYVPVGFQVRFSKKKNFANKLCFFIEGRGGVNMMK
                     VPGLGTRTSPFGIYMGGFRFCFENTQ"
     gene            1736..2617
                     /locus_tag="VFU15_13230"
     CDS             1736..2617
                     /locus_tag="VFU15_13230"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEU4718797.1"
                     /translation="MSNLTQRLITGSIFVAVLVSAIWFSVWSCAALFLLIAILGLLEF
                     YRLSEKAGAHPQKFAGTFIGACVIVAAFLLKSGMHPSVLTPVAMLVFLLFFAELFRKK
                     ENPFAGLAWTMLGIIYIAVPFAMLVYKSVPPKQLSGPFMTPAAGVYDPRPVLMIFILI
                     WISDSMAYVCGRLFGKHKLFERISPKKTWEGFTGGLLFTGVFAFFICGWMMKENCTME
                     TKIMWVITGVVISITGTLGDLTESLFKRSIGVKDSGTILPGHGGILDRFDALLLATPF
                     ALFTQYVFTNYLLHRLF"
     gene            2797..4053
                     /locus_tag="VFU15_13235"
     CDS             2797..4053
                     /locus_tag="VFU15_13235"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009194106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HEU4718798.1"
                     /translation="MKAPVHASMFHAVLARLDKAAELFGLAPDVAEILRHPKKEVAVS
                     LPVKMDDGSVKVFEGYRTVHSTHLGPSKGGIRYAMDVNADEVRALSAWMTIKCAIANL
                     PYGGGKGGVKCDPRKLSVGELERITRAYTRAMADVFGENKDVPAPDMGTGKREMAWIL
                     DEFNRIKGEDCPGVVTGKPITLGGSRGREAATGRGIMVAALAAMKKTGINYKTATAAV
                     QGFGNVGSNAARLLASKNVKIVAISDHTAAFHNSNGIDIDKAVRHVAENGGVLKGFKF
                     GEEIAKEDILRAEVDLLVPAAIQGVITTANAKDIKAKLIVEGANGPTDAEADPILNDK
                     GIVVVPDVLANGGGVTVSYFEWVQNKYGHYWSEDEVNAKHDASMSQAFENVWFNAQQF
                     KTSMRIGAYITALKKLDKAIRYKGNY"
BASE COUNT         1182 a         1236 c         1207 g         1083 t
ORIGIN      
        1 cgaagtgaac atcaacccgc cgtacagcgg ccctgccgac acgatccacc cggcgcagaa
       61 cagcaccgga acggaatccg gcccggagcg gacagatacc tgatgcggca gcgcgtcttt
      121 tttctacctt aggatttcaa atggacgaaa gcaccagccg ggaaaagatc ctcaagaaag
      181 ttcgtgcggc gctgatcaac aaatcgcggc aggaaccttc ccccgtcgat cttgaaagca
      241 atgtttttga atcgccttcc gacagcatag agctcgtttt tgcacaacag ttttccgatg
      301 caggcggaca attcgtcttc tgtgagaacg aagaagaatt tgcgtacaac atcggcgcgc
      361 tgatcgcgga cggaagcttc gggaaaatat ggtgcgcgga aaaagaatgc caggaacttc
      421 tttcgcaggc cgggctcaaa ttcctttcca aagaaaaaga catcgcatcg gcgcagacat
      481 cactcaccgg ctgtgaattt ctcatcgcgc gcacgggcag cgcactggtg agctcacgcc
      541 aggcttccgg gagaaaaggc catgtgtttc ccgacagtca cattatcgtg gcgaaaacct
      601 cgcagctggt gatgcatgtg aaagatgcat tgcgtgagat gcgcaaccgt tacccttcgg
      661 ggcttcccgg catgctcacg atgatcacgg gacccagccg caccgccgac attgaaaaaa
      721 cgctcgtgca gggcgttcac ggaccgaaag aaatttacgt cttcctgatc gacgatgttc
      781 cgcaggaaaa cgtgcaggaa taacccgacc ctgatgaaaa agttcctcgt tccacttctc
      841 ctcgccatat ccgttccggt cctggcgcag gacagcacac gtgtcccttt ttttaaaccg
      901 gaagccatcg ggttctattc cggaatcact gctttccgcg atgtgccggt aaccatttcc
      961 gacctgcaga agatcgcacc ttcttccgcc ctgctcggcg ccgacctgga ttctttcaaa
     1021 acgaacacat tcaccggttc cgggaatgtc aattcggttt tcggaatggg cgtcgatttc
     1081 aggtaccgca tgagatcgaa cggacaatac gccggctttt caagaatacg cgccagcatt
     1141 atgtacacga atggtcaaac ggcgtacagg gaattttaca ggaacagcac cgtgcgcacg
     1201 gacaccgtca gactctcgaa cggacagcag ttctatgtcg attcgatcca caatgaatat
     1261 tacacgtatt catggaatta ccaggcgtta ggcattgacc tcaaccaggt tttccacacg
     1321 aaagacgcac gcattttttc agcctacacc ggttacggca ttcatttcgg cctgatcatc
     1381 ggaagctatt tcagcgcgac gcacgagctg cagaccggtt cgcagttcct tacgacggat
     1441 tccaccgcaa cgggcagcag catgggctat ttcacaacga atacaaggca ggatgaaacg
     1501 gaaaacatcg cgacgaaaaa ctactacact ttttcctttt atgtgcccgt cggtttccag
     1561 gtgcgtttct cgaaaaagaa gaacttcgca aacaagcttt gctttttcat cgaaggcagg
     1621 ggcggtgtaa acatgatgaa agttcccggc ctcggcacac gcacaagtcc gttcgggatc
     1681 tacatgggcg gattccgttt ctgtttcgag aacacgcagt aagcgccttt cagctatgtc
     1741 gaatctcacg caacgtctca tcaccgggag cattttcgtc gccgttctcg tcagcgccat
     1801 ctggttcagc gtgtggagct gcgccgcatt gttcctgctg atcgccattc tcgggctgct
     1861 ggaattttac cggctttccg aaaaggcagg agctcatcca cagaaattcg ccggaacatt
     1921 catcggtgcc tgtgtcatcg tcgccgcatt cctgcttaaa tcgggcatgc accccagcgt
     1981 gctgacaccg gttgccatgc tggtgttcct gctttttttc gcggagctgt tccggaaaaa
     2041 agaaaacccg tttgcaggtc ttgcgtggac aatgctcggc attatttata tcgccgttcc
     2101 ctttgcgatg ctcgtgtaca aatcagtgcc accgaagcag ctttccggtc cattcatgac
     2161 accggctgcg ggcgtttacg atccgcgtcc cgtgctgatg atcttcatcc tcatctggat
     2221 cagcgattca atggcgtacg tgtgcgggcg tttgttcggt aaacacaaat tattcgagcg
     2281 catctcgccg aaaaaaacct gggaaggatt taccggcgga cttctcttca ccggcgtttt
     2341 cgcatttttc atttgcggat ggatgatgaa agagaactgc acgatggaaa cgaagatcat
     2401 gtgggtgatc accggcgtgg tcatttccat cacgggcaca ttgggcgatc ttaccgaatc
     2461 acttttcaaa cgcagcatcg gcgtgaagga ttccgggacc attttacccg gtcacggcgg
     2521 cattctcgac cgtttcgacg ccctgctgct ggcgacgcct ttcgccctgt tcacgcagta
     2581 cgttttcaca aattaccttt tgcaccggct tttttagcga tgcgcttcag cgggttaatc
     2641 gacagctttc actcattcat ctgacaatca ggctcttttc gactgaaaca ggaagttgca
     2701 atcgattaaa aaacattaaa ttcgtctccc catatcacga gataacgaac caaaaaaaca
     2761 attatgtcaa caacagcaaa gaaagcggag gaaacgatga aagcacccgt acatgcgagt
     2821 atgttccatg cagtactcgc ccgcctggat aaagcggccg agcttttcgg tcttgcaccg
     2881 gacgttgcag aaattctccg ccacccgaag aaagaagttg cagtgagcct tcctgtcaaa
     2941 atggacgacg ggtcggttaa agtttttgaa ggttaccgta ctgttcattc cacgcacctc
     3001 ggcccgtcga aaggcggcat ccgttatgcg atggacgtga acgccgatga agtccgtgcg
     3061 ctgtccgcgt ggatgacgat caaatgcgcc attgccaatc tcccgtacgg cggaggaaaa
     3121 ggcggcgtga aatgcgatcc gcggaaatta tccgtgggcg agctcgagcg catcacgcgt
     3181 gcctacacgc gcgccatggc agatgttttc ggcgagaaca aagatgttcc ggctcctgat
     3241 atgggaacgg gcaaaaggga aatggcgtgg atcctcgatg agttcaaccg gatcaaagga
     3301 gaagattgcc cgggcgttgt aaccggaaag cccatcacgc tcggcggctc ccgcggacgt
     3361 gaagcagcca caggacgcgg catcatggtc gctgcacttg cggcgatgaa aaagaccggc
     3421 atcaactaca aaacagcgac tgcagcagta cagggattcg gcaatgtcgg atcgaatgca
     3481 gcacggctgc ttgcttcgaa gaacgtgaag atcgtagcga tctccgacca tacggcggct
     3541 ttccataatt cgaacgggat cgacatcgac aaagcggtac gccatgttgc agaaaacggc
     3601 ggcgtgctga aaggattcaa attcggcgaa gagatcgcga aagaagatat tctccgcgct
     3661 gaagtcgacc tgctcgttcc tgccgccatc cagggcgtga tcacaacggc gaatgcgaaa
     3721 gacatcaagg caaagctgat cgtggaagga gcgaacggtc ctaccgacgc agaagcagac
     3781 ccgatcctca acgacaaagg catcgtggtt gttcccgacg ttcttgcaaa tggcggagga
     3841 gtaaccgttt cctatttcga atgggtgcag aacaaatacg gccattactg gagcgaagac
     3901 gaagtgaacg caaagcacga cgcgagcatg tcgcaggctt ttgaaaatgt ctggttcaat
     3961 gcgcagcaat tcaaaacgag catgcgtatc ggcgcataca tcacggcgct gaagaagctg
     4021 gacaaagcga tccgctacaa agggaattat tgattgagca ttttgaatgc tgactaaggc
     4081 cggggagatc tccccggctt ttttgttgtt tcatcttgca agaaaaccgc cggatgcatt
     4141 tgttgctttt atgtttgatt ttccattcca ctatgaaatc ccgcctgatc ttttttacaa
     4201 ggttggcttt ttgccgtgag gattccggcg cagtccggcg gaaattattt cagcgcatcc
     4261 gaccgtacac taaacccgga aaaggacagc tgcttttttc aggatgaaaa gaaaatatgg
     4321 ggatcgattg atcaatggtg tactgtaacg tttgataatt catgtaccga taaccggtcc
     4381 atgaaatgca tttaacaatc cgaaacaggc gtcatacggc tctccccgaa aacggtgact
     4441 ggtgtttttt tcgtaccggc ttcgacatga tgctttatga agaatgcttt cgcgcggaac
     4501 ggtttacccg gctgaaaatg gaagttaggt gaattagggg aagaggattg catgatggcc
     4561 tggctggggt agcaacctgc aaggttagga cctgcaaggt taggacctgc aaggttataa
     4621 cctgcaaggt tgtattataa aataacacac ccggttaacc tcttattatt ctttaactta
     4681 cacctctctc tctccaaatt caccccaa
//
LOCUS       DASTSF010000455         8638 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_516649,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000455 DASTSF010000000
VERSION     DASTSF010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 8638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8638
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_516649"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..720)
                     /locus_tag="VFU15_13240"
     CDS             complement(<1..720)
                     /locus_tag="VFU15_13240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016200248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU4718799.1"
                     /translation="MAIRSRLVVMSFLQFYVWGAWLITVANYWFGTKGWQPAEFGAIF
                     STLGLSSLFMPTITGIVADRWINAERLYGILHVLGGITLACIPFVNDPGTFFWVIFLA
                     MICYMPTISLSNSVAYHILKSNSLDVVRVFPPIRVWGTVGFIAAMWTTNLTGNKATAN
                     QFYIAAVAAVLLGIYSFTLPECPPHGSDGKSRSLSEAIGLKAFRLFADRKMALFFLFS
                     MLLGAALQLTNMYGDRYLSHFK"
     gene            complement(773..1402)
                     /locus_tag="VFU15_13245"
     CDS             complement(773..1402)
                     /locus_tag="VFU15_13245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008510259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HEU4718800.1"
                     /translation="MSEGNKKIKLEIVGLSYSQTQSGAYALVLGESRGKRRLPIIIGG
                     FEAQAIAIELEKMTPSRPLTHDLFKSFAEAFSIDLHEVLIYNLVEGIFFARLVCSRAG
                     EEVEIDARTSDAIALAVRFECPIYTYEFILSQAGIILEDETPKALEKETGEETSEQEE
                     ELLAEASGGPDYNRKTTKELEEMLRIALEEENYERASRIRDELSRRRSA"
     gene            complement(1452..2432)
                     /locus_tag="VFU15_13250"
     CDS             complement(1452..2432)
                     /locus_tag="VFU15_13250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HEU4718801.1"
                     /translation="MSVLVFTESAKGKIRKNSLEAVNYAANIASQLGTKVVAVIDGAT
                     DPAALADLGKAGASKVLTITSDKLKADTAAWAAALEQAAAAEGAQVVVFSHDLMGKAI
                     APRLAVKMKAGIVAGAVDHPTNEGGKMSVRKTVFSGKATAVYTFHSDKKIITLLPNSF
                     QVKMDGAAPEISAFAANLGSIASRITVKEVKTRTTTTVALPEADIVVSAGRGMKDPKN
                     WGIVEDLASALGAATACSRPIADMGWRPHHEHVGQTGITVRPNLYIAIGISGAIQHLA
                     GVNGSKTIVVINSDKEAPFFKAADYGVNGDLFEVVPKLTEAVKKFKASQH"
     gene            complement(2453..3196)
                     /locus_tag="VFU15_13255"
     CDS             complement(2453..3196)
                     /locus_tag="VFU15_13255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HEU4718802.1"
                     /translation="MKILVCISQVPDTTTKIAFTPDNKQFQKSGVQFIINPYDEWYAL
                     VRALELKEAKGAGSVTLIHVGPAENEAIIRKGLAIGADDAVRIDADPADAYFVAEQIS
                     AYAKDKSFDLILTGKETIDSNSSMVGGMIAEMLDLPFVSLATKLDVNGTEATTECDID
                     GGTEVLACQLPAVISCQKGMAEARIPNMRGIMAARTKPLAVVPVSNATVMTSVESYRL
                     AEGRKAVKMIDPNNMDELVSLLHTEAKVI"
     gene            complement(3388..4458)
                     /locus_tag="VFU15_13260"
     CDS             complement(3388..4458)
                     /locus_tag="VFU15_13260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014202590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEU4718803.1"
                     /translation="MARFHKLKVKEITHETADCVSVVFDVPASLKDEFRYLAGQYLTL
                     KIPSGGEDVRRSYSLCSSPVADTEYRVAIKKVKEGRGSVYINEKLKVGDELEVMTPMG
                     NFHSPMDEAHAKQYVLFAGGSGITPMLSILKTVLKAEPQSTVTLIYGNRDEQSVIFKK
                     TIDTLAAENKERFEVIHVLENPPAGTDERFRGLLTEEKVRRLLEENLRLNADNEFFIC
                     GPGPMMENVKAVLTAMNVDPKRIHIEYFTAVAEAVKAAEAEGARIVSKVTIIMDGEEL
                     NVDLASDGKVILDVAIDGGLDAPYACKGAVCCTCRAKILEGKMRMDQNYALSDEEIEQ
                     GYILTCQAHPVSERVIVSYDEP"
     gene            complement(4832..7678)
                     /locus_tag="VFU15_13265"
     CDS             complement(4832..7678)
                     /locus_tag="VFU15_13265"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEU4718804.1"
                     /translation="MTRQLFFLSSIIFLASGINGQVTQQWVENYNGTVSGNDRAVAMK
                     VDAVGNTYVTGYSQNGSSGNDYTTVKYNSSGVQQWVATYNGTGNNNDYATAIAIDGSG
                     NTYVTGYSMNTGYTNDYVTIKYNSSGVQQWAAVYTGVSNYAQAIAVDAAGNSYVTGYA
                     HDGSSFNYATVKYNSSGVQQWVAIYNGTGNGPDMATAIVVDGAGNTYVTGNAFNGSDY
                     DYATVKYNSAGTQQWVAIHNGAGNNADYAKAIAADASGNIYVTGNCYNGSNHDYVTIE
                     YNSSGAQQWIGIYNGPANSTDQANAIAVDGSGNTYVTGFSFNGSNYDFATVSYNSSGA
                     QQWAAVYAGGGNADDRANVIALDAGGNVYVTGMRNGGDYATIKYDNGGAQQWIISYTG
                     TAGSYDEAFSIGLDAMGNVYVTGYATNTANGNDYATIKYSQPPPLSFTQSETDLSCNG
                     ICNGEASVVATGGTAPYTYAWSSGGTAATESGLCAGNYTCTITDASSVTVTATFDITE
                     PSALTSTSSATSVLCNGGTSTVTVAASGGTSPYSGDGTFSETAGTYSYTVTDFNGCTN
                     TSSVTITEPSALTSTSSATSVLCNGGTSTVTVAASGGTSPYSGDGTFSETAGTYSYTV
                     TDFNGCTNTSSVTITEPSALTSTSSATSVLCNGGTSTVTVAASGGTSPYSGDGTFSET
                     AGTYSYTVTDFNGCTSTSSVTITEPAQLFVAVTATTDPATCAGSDGAIDITVSGGTPV
                     YTYQWSNSSTNEDPSGLTAGTYSCTITDADGCTAAGNATLTDPNPPAVTVTFSQDTVC
                     QADGAYALSGGLPAGGAFSGPGVTSGFFDPGAVSLGMNPISYTYTDMTTGCSSTAADS
                     IDVELCTGMDAPHREISFTLLPNPNNGIFLVEIDGEQAVDVVICDALGQLISAQKVEP
                     HVGQQFSLANPGVYFVAVIMPDGRRSCQRVMVNR"
     gene            complement(7776..8162)
                     /locus_tag="VFU15_13270"
     CDS             complement(7776..8162)
                     /locus_tag="VFU15_13270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718805.1"
                     /translation="MKKTRMIFTAAVVLLIASSFTLKSVSADYANCFVVSETKWGEQC
                     GNPKSFKIKFRNTCDVKMDIKYALKQTDGTWSCGIESNVGPFQETGDGAWACECTGDY
                     KWWARPSDKRGEIDFPTNEDINSGNY"
BASE COUNT         1911 a         2334 c         2184 g         2209 t
ORIGIN      
        1 tttgaaatgg gaaaggtaac ggtcgccgta catgttcgtc agctgcagcg cagcgccgag
       61 cagcatggag aacaaaaaga agagcgccat tttcctgtcg gcaaacaggc ggaaggcttt
      121 cagcccgatc gcttcggaaa gcgacctgct cttcccgtcg ctgccgtggg gcgggcattc
      181 cggaagggtg aacgaataaa ttcccagcag cacggcggcc accgcggcaa tgtaaaactg
      241 gttcgcggtc gctttgttgc ccgtaaggtt tgtcgtccac atcgctgcaa taaaacccac
      301 cgttccccac acgcggatcg gcggaaacac acgaacaacg tcgagtgaat ttgatttcag
      361 gatgtggtag gcaacggaat tcgaaaggga gatcgtcggc atgtagcaga tcatcgccag
      421 gaaaatgacc cagaagaacg tgccgggatc attgacgaac gggatgcacg ccagcgtaat
      481 tccgccaagc acgtgaagaa tgccgtacag tctttccgca ttgatccacc ggtcggccac
      541 gatgcccgta atcgtcggca tgaaaagcga ggaaaggccc agcgtcgaaa atatggcgcc
      601 gaactcggcc ggttgccagc ctttggttcc gaaccagtaa ttggcaaccg tgatcagcca
      661 ggctccccag acgtaaaact ggagaaagct catcactaca agccgggatc taatggccac
      721 agatttttta ttgggaatac taaactaagc ggaaaaacgg gaatggaaaa gtttacgccg
      781 atctgcgccg gctcagctca tctcggatgc gtgaagcgcg ttcgtaattt tcttcctcga
      841 gcgcaatgcg cagcatttcc tccagttctt tcgtcgtctt gcggttgtaa tcaggaccac
      901 cggaagcttc ggcgagaagc tcttcttcct gctcgctggt ttcttcgccc gtttcctttt
      961 cgagagcctt cggcgtttca tcttcgagga tgatgccggc ctgggaaagg atgaattcgt
     1021 aggtgtagat ggggcactcg aaccttacgg ccagcgcgat cgcatcggaa gtgcgtgcat
     1081 cgatctccac ttcttcaccc gcacggctgc agacgaggcg cgcgaagaaa attccttcca
     1141 cgagattata aatgagcact tcgtgcaggt cgatcgaaaa cgcttcggca aaacttttga
     1201 aaagatcgtg tgtaagcggg cggctcggcg tcattttttc cagctcgatg gcaatggcct
     1261 gcgcttcgaa tcccccgata ataatgggaa gacggcgttt tccgcgcgac tcccccagca
     1321 cgagagcgta tgcccccgat tgtgtctggc tgtacgaaag accgacgatc tcgagcttta
     1381 ttttcttgtt tccttcactc acaattccgg ggagaatttg aagtattaaa tatacgcatt
     1441 actcccggaa atcagtgctg ggaagccttg aactttttga ccgcttccgt caatttcggc
     1501 acaacctcga aaagatcgcc gttgacgccg tagtcggccg ccttgaagaa aggcgcttcc
     1561 ttatcgctgt tgatgacaac aatggttttg cttccgttca cgccggcgag gtgctggatc
     1621 gccccggaaa tcccgatggc gatgtacagg ttcggacgaa ccgtgatgcc cgtctgcccc
     1681 acgtgctcat ggtgcggacg ccatcccata tctgcgatcg gccgcgagca ggcggtggcg
     1741 gcgccgagcg cggacgcaag gtcttccacg atgccccagt tcttcgggtc tttcattcct
     1801 cgtccggcgg aaacgacgat atcggcttcg ggaagggcca cggttgtcgt ggtgcgtgtt
     1861 tttacttcct tcaccgtgat gcgtgatgcg atgctgccga ggttcgctgc gaatgcagaa
     1921 atttccggcg ctgctccgtc catctttacc tggaaggaat tcggcaggag cgtaattatt
     1981 tttttatcgg aatggaacgt ataaacggct gtagccttcc cggagaaaac cgttttgcgc
     2041 accgacattt ttccgccttc gttcgtggga tgatcgacgg ccccggcaac gatgcccgct
     2101 ttcattttta cagcgagacg gggagcgatg gcttttccca taaggtcatg cgaaaacacg
     2161 acgacctgcg caccttccgc cgcagcggcc tgttccagcg ccgcagccca cgcagcggta
     2221 tccgctttga gcttgtcgct ggtaatggtc agcactttcg aagcgccggc tttgccgagg
     2281 tccgcaagcg cggcaggatc cgtggcaccg tcaatcacgg caacgacttt cgtacccagc
     2341 tgcgatgcga tattcgccgc gtaattcacg gcttcgagcg aattcttgcg gatctttcct
     2401 ttggcggatt cagtgaaaac taaaacactc atgtcggttc tttgtaataa ggttaaatga
     2461 ctttggcttc cgtgtgcagc agtgaaacga gctcatccat gttgttcgga tcgatcatct
     2521 tcacagcttt ccttccctcg gcgagacggt agctttcgac cgatgtcatg accgttgcat
     2581 tgctcacggg aacaacggcc agcggtttgg tgcgcgcagc catgatcccg cgcatattgg
     2641 gaatgcgcgc ttccgccatt cctttctggc agctgatcac agccgggagc tggcaggcca
     2701 gcacttccgt tccgccgtcg atgtcgcatt ccgtggtggc ttccgttccg ttcacatcga
     2761 gcttcgtggc cagtgaaacg aatggaagat ccagcatttc ggcgatcatc ccgcccacca
     2821 tcgagctgtt cgaatcgatg gtttcttttc ccgtaagaat gagatcgaac gacttgtctt
     2881 ttgcataagc ggagatctgc tccgccacga aataagcgtc tgccggatcc gcatcgatac
     2941 gcacagcatc atccgcaccg atcgcgagtc ctttgcggat gatcgcttcg ttctcggcgg
     3001 gtccgacgtg gatcagcgtc accgatcctg ctccttttgc ttccttgagc tcgagcgcgc
     3061 ggaccagcgc ataccattcg tcgtacgggt tgatgatgaa ctgcacaccg gatttctgga
     3121 actgcttgtt gtccggtgtg aacgcaattt tagtggtcgt atcaggcacc tggctgatgc
     3181 agactaatat tttcattgga aatcgtgttt ggcgttcgtt aaacttccgc tgcggaaatt
     3241 tccgttcagc gaggagcaaa aataaggttt tcgtgcgaaa gggaaacgcc gttttccggg
     3301 agatgatttt tccttccagc acgtatggcc tgcagacgga taacaatgcg gtttttcatt
     3361 cgcaggccgg agaattccag gggaaaatca gggttcgtcg tagctgacga tcacgcgctc
     3421 cgagacagga tgcgcctggc aggtgagaat gtatccctgc tctatttcct cgtcagacag
     3481 cgcatagttc tggtccatgc gcatttttcc ttcgagtatt ttcgcacggc acgtgcagca
     3541 caccgcgccc ttgcaggcat acggcgcgtc gagtccgccg tcaatggcga catccaggat
     3601 cacttttccg tcggaagcaa ggtcgacgtt cagctcttcg ccatccatga tgatcgttac
     3661 cttgctgaca atgcgtgcac cttcggcttc cgcggctttc accgcttccg cgacggctgt
     3721 gaaatattcg atgtgaatgc gtttcggatc aacgttcatc gcggtgagaa cggccttcac
     3781 gttttccatc atcggcccgg gaccgcagat aaaaaattcg ttgtctgcat tcagccggag
     3841 attttcttcc agcagcctgc gcactttttc ttccgtgaga agtccgcgga aacgttcatc
     3901 ggtaccggcc ggaggatttt ccagcacgtg tatcacttca aaacgttcct tgttttccgc
     3961 tgccagcgtg tcgatcgttt ttttgaaaat gacggactgc tcatcgcggt tcccgtaaat
     4021 aagcgtgact gtactttgcg gttcggcttt caataccgtt ttcaggatgg aaagcatggg
     4081 cgtgatgccg ctgccaccgg caaaaagaac atactgtttc gcatgcgcct catccatggg
     4141 tgaatgaaaa ttccccatcg gcgtcatcac ttccagctca tcaccgacct tcagcttttc
     4201 attgatgtaa accgagccgc gcccttcctt cactttcttg atcgccacgc gatattcagt
     4261 atcggccacc ggactgctgc agagcgaata cgaacgccgc acgtcttccc cgccggaagg
     4321 aatcttcagc gtcaggtact ggcctgcgag gtagcggaat tcatctttca gcgacgcagg
     4381 aacatcgaac acaaccgaca cacagtcggc tgtctcgtgc gtaatctcct tcactttcag
     4441 cttatgaaac ctggccatta atttggattt ctcagggcgc aaaaataaga ttctgaacgg
     4501 gtttttggaa ggtaatttca actgcctgtt ttccagctgt ttaagaatag cgattatgat
     4561 ccgcttgagc gccgcagtac agatttaacc ggcgctgtaa attaccctgg cttttcacca
     4621 gcgaccggga acattccctg gtactaacgg atcgtattgt tcctgtgaga agcgggcccc
     4681 gtgaatgagc cggctgaaaa aacttcaccg cttccttgca ggcatgttcc ggagggaatg
     4741 cgtgtggttt cgtccacgta tcgcggattg aagctttcga tgctgatcaa attccgtacc
     4801 tgcagttcct cccggccggc gctgaaaacg atcacctgtt caccatcaca cgctggcagg
     4861 agcgacgtcc atcgggcata attaccgcga cgaagtaaac tcccggattg gcaagactga
     4921 attgctgccc gacatgaggt tctactttct gtgcgcttat caattgtccc agtgcatcgc
     4981 agatcactac atcaaccgcc tgttccccgt cgatctctac caggaagatt ccgttgttcg
     5041 ggttcggcag cagcgtaaaa ctgatttccc ggtgcggggc atccattcct gtgcaaagct
     5101 ccacatcgat cgaatcggct gctgttgagg aacagccggt cgtcatatct gtgtacgtat
     5161 agctgatggg attcattccc aggctgacag ccccgggatc aaaaaaacct gatgtaacgc
     5221 ccggtccgga gaatgctccg cctgcaggca aacctccgct taatgcataa gcgccgtctg
     5281 cctggcaaac agtatcctgg gaaaacgtaa ctgtaactgc gggcggattg ggatccgtca
     5341 gtgttgcatt gccagcagcg gtgcatccgt ctgcatctgt aatcgtgcaa ctgtaagttc
     5401 ccgcagtaag accagacgga tcttcgttcg tgctgctgtt gctccattga taagtataga
     5461 caggtgttcc gccagaaacg gtgatgtcga tcgcgccatc gctgcctgca caggttgcag
     5521 gatctgtggt ggctgttact gcaacgaaaa gctgcgcagg ctcggtgatc gtgacggaac
     5581 tcgtgctcgt gcaaccattg aaatccgtta ctgtgtatga atatgttccc gcggtttcgg
     5641 aaaatgttcc gtctcctgaa tacggtgaag ttccgccgga cgccgcaacg gtgactgttg
     5701 aagttccgcc attacacaaa acagaagtgg ccgaggaagt cgatgtaagc gccgacggtt
     5761 cggtgatcgt gacggaactc gtgttcgtgc aaccgttgaa atccgttacc gtgtaagaat
     5821 acgttcctgc tgtttcagaa aacgttccat cacccgaata cggtgaagtt ccgccggacg
     5881 cagcaacggt gactgttgaa gttccgccgt tacacaaaac agaagtggca gaagaagtcg
     5941 atgtaagcgc cgacggttca gtgatcgtaa cagaactcgt gttcgtgcaa ccgttgaaat
     6001 ccgttaccgt gtaagaatac gttcctgctg tttcggaaaa tgttccatcg cctgaatacg
     6061 gtgaagttcc gccggacgca gcaacggtga ctgttgaagt tccgccgtta cacaaaacag
     6121 aagtggccga ggaagtcgat gtaagggctg acggttcggt gatatcgaaa gtcgcggtaa
     6181 ccgtaacgga tgaggcgtcc gtaattgtgc aggtgtaatt cccggcgcaa agaccgcttt
     6241 cggttgcggc cgttccgccc gaagaccagg cataagtata cggggcagtt ccgccggttg
     6301 caactacgga agcttcgccg ttgcagattc cgttacagga aagatcggtt tccgattgcg
     6361 tgaaagacaa gggaggcggc tgcgaatact tgatcgtggc gtaatcattc ccgtttgccg
     6421 tattagtagc ataacctgtt acataaacat ttcccattgc gtcaagaccg atcgaaaatg
     6481 cttcgtcata gctgcctgcc gtaccggtat atgaaatgat ccactgctgc gcacctccgt
     6541 tgtcgtattt tatggtggcg taatctcccc catttctcat tcccgtaaca taaacattcc
     6601 cgcctgcgtc aagcgcgatt acattcgccc tgtcgtcggc atttcctcct cccgcgtata
     6661 cggcagccca ttgctgcgcc ccggagctat tataactgac ggtggcaaaa tcataattgc
     6721 tcccgttgaa cgaaaacccg gttacatacg tatttccgct gccatcgaca gcgatcgcat
     6781 tggcctggtc agtgctattt gctggtccgt tataaatgcc tatccactgc tgcgccccgc
     6841 tgctgttgta ttcaatggtc acataatcat gattgcttcc attgtaacaa ttaccggtta
     6901 cgtaaatgtt cccgctggca tccgcggcta tcgcttttgc ataatctgca ttattccctg
     6961 ctccgttatg aatggccacc cattgctgcg ttccggcact gttgtacttg acggtggcgt
     7021 aatcataatc gcttccattg aaagcgttgc cggttacgta ggtattccct gcgccatcga
     7081 cgacaattgc agtcgccatg tcaggaccat ttccagttcc gttgtaaata gcaacccatt
     7141 gctgaacgcc gctgctgtta tacttgaccg tggcgtaatt aaagctgctt ccatcgtgag
     7201 catagcctgt cacataggaa tttccagccg cgtcaaccgc aattgcctgc gcataattgc
     7261 tcacgccggt ataaacggcc gcccattgct gcaccccgct gctgttgtat ttaatcgtaa
     7321 cataatcatt tgtataaccc gtattcatgg aataaccggt gacgtacgtg ttcccgcttc
     7381 cgtcaatggc gatcgcggtg gcataatcat tattgttccc ggtcccgttg taggtcgcca
     7441 cccattgctg taccccgctg ctgttgtatt tgacagttgt gtaatcgttg ccgctgctgc
     7501 cattctggga atatcccgtt acgtaggtat ttccgaccgc atccactttc atggctacag
     7561 ccctgtcatt accgcttacg gttccgttat agttttcaac ccattgctgg gttacctggc
     7621 cgtttatgcc tgaagccaga aaaataattg aactgaggaa aaataattgc cttgtcatat
     7681 ttcgttttat actgcaggaa acaacggttc cgttcgccgg aacattgcct ggtgcacggt
     7741 agtgaaacag gagtatttgt aaagcattca ggagatcagt aattcccgct attgatgtct
     7801 tcgttggtcg ggaaatcgat ttcgcctctc ttgtcggaag gacgagccca ccatttataa
     7861 tcacctgtac attcgcacgc ccatgccccg tcacctgttt cctggaaagg acctacattg
     7921 gattctattc cgcaggacca ggtgccgtcc gtttgcttga gcgcatactt gatatccatt
     7981 tttacatcgc acgtattccg gaattttatc ttgaagcttt tggggtttcc gcattgttca
     8041 ccccatttgg tttcagatac tacgaagcaa ttggcgtaat ccgccgaaac tgattttagg
     8101 gtaaacgagc ttgcgataag caataccacc gctgcagtaa aaatcattct tgtctttttc
     8161 atgattccgg ttttaatgat ttgttttttg ttgcgcgtcc cgcgaattcc agggttttcc
     8221 acacagcaga taaccgggaa catgccaggc cagcacgcat gattgatttc gggttcaagg
     8281 atattctgaa aaaaaccact cccaaatacc catttatggg tacgtaaaaa aatcacgtgc
     8341 gaagcagatc acctccgctc cggaaatgaa aacaaaatct ttccatgctg ctgaatcggt
     8401 ttttatccgc gccgctacac ttagaatgct tccgggcggt attttatacc aataagcgct
     8461 ggcacgatca caacgcgtgc aaattatttt caccatgatt gatccgctcc gtaaaccgct
     8521 ttacatttgc gctgcattac agggcaagca tcatgaaaca tccttaccta agacgtcctt
     8581 tcattttatc tttttttctt tcctgctttt tttccgcact cggcgccacc accacgat
//
LOCUS       DASTSF010000456         3183 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_517930,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000456 DASTSF010000000
VERSION     DASTSF010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3183
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_517930"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3033)
                     /locus_tag="VFU15_13275"
     CDS             complement(<1..3033)
                     /locus_tag="VFU15_13275"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEU4718806.1"
                     /translation="MRTFLRRFFENRYFPERILFLSIAGGMLLDGYISAAFVDMTAEA
                     ALRVTVSLLSLSFIPLTFRKGVQPIVIRSLAIGSIVILLLFSVYLNTRHHLDFDNALT
                     FLGIFIICSLYFRSVRSLVFYLLFGLSMAALMILFIDHPQVDAPIFLLRLFLGSLLIL
                     ALSLAMRQFQVKLERITRRVAEENRALNETKSALEERLTHEHLLALVASRANSAVIIT
                     NREDRIEWVNQFFLDMTGYSKEEIYGMSPSVLRGKKTDPEAEKRIEEKKKLAQPFHDI
                     ILNYKKDGTPFWVHLHVTPLFDEEGNVERYIAIQEDITEQKKIEDDLRKNREQLKEAQ
                     QQAKIGSWEWYDGSDIVSCSDEMCRIFGLPVHEHYPLKQVFDLIFPADLAMVRKSIEI
                     GLKKISPFEIGFRVSTTEGLKHLYLTGRAVSDGKKRTLRLFGSVQDITERKNIENEMQ
                     LAEKQYRSLFENSQHMICMHSPDGTILSINPAGAHALGFEPEELIARNIRSFFQPGKE
                     KEFEEYIRAIVSSGQSKGVLRLRKRDGSSSVWLYSNILLQGNDGMPFVLSSTVEITER
                     YAMEKELRRAKILAEEALVMKDRFVANISHELRTPLNAIIGFSDLLMNTELAPEQYEY
                     LDAVHIAADNLAAMINDVLDLAKIEAGKIEFEQKPYSIRDVLSNTHRLLSQRAKNQKL
                     DFKWECDARVPVYVLGDELRLSQVLINLVANAVKFTEKGFVHFSCSVITEEDGYYMLR
                     FDVEDSGVGIPADQLTSIFEPFNQASLESTRKFGGTGLGLSIVRDLVELQGGTVLAKS
                     APGIGSKFTVELPVKKVSVDVVQQVDYALKPIELPANVKILIVEDQPLNQQLAKRLIT
                     DFGFTPEVAFNGRMAIDFLKNNKYDLVLMDLSMPEMDGYDATRVIREKLRLDVPVIAL
                     TAHSSAGEKEKCIAAGMNDYLSKPFRGKELYLKIVGAMKKNISEKETALLDSEAETPL
                     KSLAGGDRNFEREMIEIMLRSIPEDFALMKDALQKREFE"
BASE COUNT          689 a          893 c          854 g          747 t
ORIGIN      
        1 ttcgaattcc cttttctgca acgcatcttt catcagcgcg aaatcctcgg gaatggaccg
       61 aagcattatc tcgatcattt cccgctcgaa atttctatcg ccgcccgcaa gcgatttcag
      121 cggcgtttcc gcttcgctat ccagcaatgc ggtctccttt tccgaaatat tcttcttcat
      181 cgcgccgacg atcttcaggt acaactcctt tccgcgaaaa ggcttcgaga ggtaatcgtt
      241 catcccggcc gcgatgcact tttccttttc gcccgccgac gaatgcgccg tgagcgcgat
      301 gaccggaacg tcgaggcgaa gcttttcgcg gatgacgcgc gtggcgtcgt aaccgtccat
      361 ctcgggcatg gaaagatcca tgagcacgag atcgtatttg ttgttcttca gaaaatcgat
      421 cgccatgcgc ccattgaacg cgacttccgg cgtgaacccg aaatccgtga tgagcctttt
      481 cgccagctgc tggttcagcg gctggtcttc gacgatcagt atcttcacat tcgccggcag
      541 ctcgatgggt ttcagcgcat aatccacctg ctgcacgaca tccacgctca cttttttcac
      601 cggtagctcg acggtgaact tcgagccgat gcccggcgca cttttcgcga gcacggttcc
      661 gccctgcagc tcgacaagat cgcgcacgat gctcagtccc aatcctgttc cgccgaactt
      721 ccgcgtcgac tcgaggctcg cctggttgaa cggttcgaag atcgacgtga gctgatctgc
      781 cggaattccc actcccgaat cttccacgtc gaaacgcagc atgtagtaac cgtcttcctc
      841 ggtgatcacg ctgcacgaaa aatgcacgaa tcctttttcc gtgaacttca ctgcgttcgc
      901 aacgaggttg atgagcacct gcgaaagacg cagctcatcg ccgagcacat agacgggaac
      961 acgtgcgtcg cattcccatt tgaaatccag cttctggttt ttcgcgcgct gtgaaagcag
     1021 gcggtgcgta ttcgaaagaa cgtcccgtat gctgtacggc ttctgttcga attcgatctt
     1081 ccccgcttcg atcttcgcga gatcaagcac gtcgttgatc attgcggcaa gattgtcggc
     1141 ggcgatgtga acggcgtcga ggtattcgta ctgctccggc gcaagctcgg tgttcatgag
     1201 caggtcggaa aaaccgatga tcgcattgag cggcgtgcgc agctcgtggc tgatgttggc
     1261 gacgaaacgg tctttcatga cgagcgcttc ttcagcaaga attttcgcgc ggcgcaattc
     1321 tttttccatc gcgtaccgct cggtgatctc cactgtgctg ctgaggacaa atggcatccc
     1381 gtcatttccc tgcagcagga tgttgctgta cagccacacg gaagaagacc cgtcgcgctt
     1441 gcgcaggcgc agcacgcctt tcgactggcc ggaggaaacg atggcgcgga tatattcttc
     1501 gaattccttt tctttcccgg gctggaagaa cgaacggatg ttgcgtgcaa tgagctcttc
     1561 gggctcaaag cccagcgcgt gcgctcctgc cgggttgatg ctgaggatcg ttccgtccgg
     1621 cgagtgcatg cagatcatgt gctgggaatt ctcgaacaag ctccggtatt gcttttccgc
     1681 cagctgcatt tcattttcga tatttttccg ctcggtgata tcctgcacgg aaccgaaaag
     1741 ccgcagggtg cgtttcttcc cgtcgctcac cgcgcggccc gtcaggtaaa gatgcttgag
     1801 cccttcggtc gtcgacacgc ggaagccgat ctcgaacgga gaaattttct tcaggccgat
     1861 ctcgatggat tttctcacca tggcgaggtc agcgggaaaa ataagatcga agacctgctt
     1921 cagcgggtaa tgctcatgca cgggaagacc gaaaatgcgg cacatttcat cggagcacga
     1981 aacgatatcg ctgccgtcgt accattccca ggaaccgatc ttggcctgct gctgcgcttc
     2041 tttcagttgc tcgcggttct tgcgcagatc gtcttcgatc ttcttctgct cggtgatatc
     2101 ttcctggatg gcgatgtagc gctccacgtt tccctcttca tcgaacaaag gcgtcacgtg
     2161 aagatgcacc cagaacggcg tcccgtcctt tttgtaattg aggatgatat cgtgaaaagg
     2221 ctgcgccagc ttcttcttct cctctatccg cttttccgct tcgggatccg tttttttccc
     2281 gcgcagcacg gaaggcgaca tgccgtagat ctcttctttc gaataaccgg tcatgtcgag
     2341 aaagaactgg ttgacccatt cgatcctgtc ttcgcggtta gtgatgatga cggccgaatt
     2401 cgcgcggctg gccacgagcg caagcaggtg ctcgtgggtg aggcgttcct cgagcgcgga
     2461 tttcgtttca ttaagcgcgc ggttctcttc cgccaccctc cgtgtaatcc gttcgagctt
     2521 cacctggaat tgccgcatgg caagcgaaag cgcgaggatc agcaggctgc cgaggaaaag
     2581 gcgaagcaaa aatataggcg catccacctg cgggtgatcg ataaaaagga tcatgagcgc
     2641 agccatcgaa aggccgaaca gcaggtagaa caccagcgaa cgcaccgaac ggaaatacag
     2701 gctgcagatg atgaagatgc caaggaacgt aagcgcattg tcgaaatcga gatgatggcg
     2761 cgtgttgagg taaacggaaa aaagcagcag gatgacgatg gagccgatcg cgagcgaacg
     2821 aatgacgatg ggctgcactc ctttgcggaa cgtgagcgga atgaacgaaa gcgaaagcag
     2881 gctcacggta acgcgcagcg ctgcttccgc tgtcatatcc acgaaagccg cgctgatata
     2941 gccgtcgagc agcatgcccc cggcaatgga gaggaaaagt atccgctcgg gaaaataccg
     3001 gttctcgaaa aatcgcctca ggaaagttcg catgccgttg ccaattatcc gaaagttact
     3061 caggtgggaa ggaaatagca atttcccgga ccagccttat ggtcttcaca ttcttccatc
     3121 gtaacaggct tcatttaaat gatgtgatcc cgatccgctg cgcttgcggg attaattccg
     3181 cca
//
LOCUS       DASTSF010000457         3884 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1208472,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000457 DASTSF010000000
VERSION     DASTSF010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3884
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1208472"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1318
                     /locus_tag="VFU15_13280"
     CDS             <1..1318
                     /locus_tag="VFU15_13280"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEU4718807.1"
                     /translation="TVLTGSGASTYSWMPGNLSGSSVTVNPTSTTTYTVTGTSASGCS
                     ATATISITVNSLPILNVTPTSAAFCAGGNVNLSASPTGGTFAWVPTTGLSAPNSPATN
                     AAPVTTTTYTVTRTSAVGCSRSVSVVVTVNPLPNVAASASSPSVCTGSSVTLTGSGAS
                     TYTWQPGNLSGASVSDNPASGTTYTVTGTDANGCVNTSTVGVAVNPAPNVAAAATPDT
                     ICPGSSVTLSGSGAVSYTWQPGNISGSSVTDAPSASVTYTMTGTDANGCSNTAFVAVV
                     VDTPPSAPSISVNGSLLTSSVSGASYQWFLNGSPIPGATSQSYTFTQSGSYTVEVYDA
                     RGCGSGQSAPIIATGIDAVASGDFMSVLPNPNDGHFQLNFHVSSADNYVVEIHNMLGQ
                     VVYSEALNNFSGDYKKDIDLSRFGHGVYTVRLTGGNHETVIKMMTY"
     gene            1440..>3884
                     /locus_tag="VFU15_13285"
     CDS             1440..>3884
                     /locus_tag="VFU15_13285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEU4718808.1"
                     /translation="MKKFVYAVWPLLFFAAGVFAQSDNNRWTDLWQTPAVNYYSVKHA
                     FDSVYADKDEELHALKGTQATGSGDRDENEDLDGMYFQFKRWEYFMQPRLGPNGETTL
                     PATTYQRFMDYLQSNPAAMAQHNASVARTATSNSWSFVGPTGAPTNSGAGRVNCVRFD
                     PNNTNIIYIGAPAGGLWKSINGGSTWTCLTDYLPVIGCSDVAIDPTNSNIIYIATGDR
                     DAGDSPSIGVMKSTDGGITWNTTGLMFNNNQGRKVARLLIDPGNPNILFAATSGGIYK
                     TQDAGVNWYQMSSLNTMDMEFKPGDPNTIYACKTALYTSTNGGITWSIAAGLPPGNQL
                     TRISVAVSPAAPDNVYVLASLASDYGYYGLYVSTNDGVSFSQRNGSPNLLGWNADGGD
                     SGGQGWYDLSIAVAPYDANTIIVGGVNIWRSDDGGYNFYLNAHWYGGGGAPYVHADCH
                     DLIFEPGTSGSYFAGCDGGIFHTGNDGASFEDLSNNLCIAQIYRVGLSQQNAGTLITG
                     HQDNGTNVKVGSNYFSGLGGDGMDCFIDRTNDNNMFGELYYGDFYRSTNGGASWNGIT
                     NGLTGSGGWVTPWVQDPVDPNTLYAGYDQVFRSTDLGSNWSPTGSTMIAATDIQVAPS
                     NHNYLYVTSGLTLLRSTDAGATFSNITFGINSGGASISRIAVCPYDEKKLWVSISGYN
                     NNQKVYYSSDAGATWTNISYGLPNIPANCITAVPGSMSDAIFVGCDVGVYYHDNSMAG
                     WQPYFTGLPNVPVYDLKIYKPAMLLRAATYGRGVWECQIDQSVLVPQAGFSANATSVC
                     PGTPVQFTDLSTLNPVA"
BASE COUNT          856 a         1129 c          990 g          909 t
ORIGIN      
        1 cacggtgctc accggaagtg gcgcttcaac ttacagctgg atgcccggaa atctttccgg
       61 atcatctgta acggtaaacc cgacttccac aacaacctat accgtcaccg gaacaagcgc
      121 cagcggatgt tcggcgaccg caacgatttc cattaccgtt aattctttgc cgatcctcaa
      181 cgtcacgcct acctccgccg ctttctgcgc cggaggaaat gtgaacctca gcgcttcgcc
      241 taccggcggt actttcgcct gggtgcccac aaccggcttg tctgccccca attccccggc
      301 tacgaatgct gcgccggtta ctacaactac ttacaccgtc accaggacga gtgccgttgg
      361 atgttcccgc agtgtaagcg ttgtcgtcac ggttaacccg ctgcccaacg ttgcggcaag
      421 cgcttcttct ccgtccgttt gtaccggaag ctctgttacg ctcacgggaa gcggcgcgtc
      481 cacctacacg tggcaacccg gaaacctcag cggcgcgtcc gtcagcgata acccggcgtc
      541 gggcactacc tacaccgtga ccggaacgga tgccaatggc tgcgtgaata catccactgt
      601 cggtgtcgct gtcaacccgg cgccaaatgt tgcagcagca gcaacgccgg atactatctg
      661 tccgggatcg tcggttacgc tttccggttc cggcgcagtt tcctacacgt ggcaacccgg
      721 aaatatttcc ggatcttcgg tgacagatgc accgtctgct tccgtcacat acacgatgac
      781 cggaacggat gcaaatggat gttcgaatac agctttcgtt gctgttgtcg ttgacacgcc
      841 gccttccgcg ccctcgatct cggtcaatgg ttcgcttctc acttcttctg tttcaggagc
      901 aagctaccaa tggttcctca acggaagccc gatacccggc gcaacgagcc agtcttacac
      961 attcacccaa agcggatcgt acaccgttga agtgtatgac gcgcgtggtt gcggttccgg
     1021 gcaatccgcg cccatcatcg caacaggaat tgatgctgtt gcatcgggtg atttcatgtc
     1081 ggttcttccc aacccgaacg acggtcattt ccaattgaac ttccatgttt cttctgcgga
     1141 taattacgtg gttgaaattc acaacatgct cggccaggtt gtttattccg aagcgctgaa
     1201 caacttcagc ggcgattaca aaaaggatat cgacctgtcg cgtttcggtc atggcgttta
     1261 caccgtacgg cttaccggtg gaaatcacga aaccgtcata aaaatgatga cctactgagc
     1321 accgtcggac gatccggaga aagccgctct catgcggctt tttctttttc gcgatttatg
     1381 gtcctccata ctgtttctca tgtcaaatct cccgatcttt cggggctcaa cattctcaaa
     1441 tgaaaaaatt cgtttatgct gtgtggccgc ttttgttttt tgctgcgggc gtgtttgcac
     1501 agtctgacaa caaccgctgg accgatctct ggcaaactcc tgcagtcaat tattattccg
     1561 tcaagcacgc gttcgattct gtttatgcag acaaagacga ggagctgcat gcgctgaagg
     1621 gaacacaagc caccgggtcg ggcgaccgcg acgaaaacga ggatctcgac ggcatgtatt
     1681 ttcagtttaa acgctgggaa tatttcatgc agccgcgcct cggtccaaac ggagaaacaa
     1741 cactgcccgc cacgacatac cagcgcttca tggattacct gcagagcaac cccgcggcaa
     1801 tggcgcagca caacgcatcc gtcgcgcgta ccgccacgag caactcctgg agctttgtcg
     1861 gacccaccgg cgcgccgacc aactccggcg ccggacgtgt caactgcgtc cgcttcgatc
     1921 cgaacaacac gaacatcatt tacatcggcg caccggcggg tggactttgg aaatcaatca
     1981 acggtggaag cacgtggacg tgtctgacag attatcttcc cgttatcggg tgttcggatg
     2041 ttgccattga tcccacgaac tccaatatca tttacatcgc gaccggcgac cgcgatgccg
     2101 gtgattcccc gagcatcggt gtaatgaaaa gcaccgacgg cgggatcaca tggaatacaa
     2161 ccggtctgat gttcaacaat aaccagggaa gaaaagtggc gcgcctgctc atcgatcccg
     2221 gcaacccgaa tattcttttt gccgccactt ccggaggcat ttacaaaaca caggatgccg
     2281 gcgtcaactg gtaccagatg tcttcgctga acacgatgga catggaattc aaacccggtg
     2341 atcccaatac gatttacgcc tgcaagacgg cgctttacac atcaaccaac ggcggcatca
     2401 cgtggtccat tgcggcggga cttccgcccg gcaaccagct gacgagaatt tccgttgctg
     2461 taagccccgc tgctccggat aatgtgtatg tgctcgcttc gcttgcttcc gattatggtt
     2521 attacggcct ttatgtttcc accaatgacg gcgtctcctt ttcgcagcga aacggttcac
     2581 cgaacctgct cgggtggaat gcagacggcg gtgattccgg cggacaaggc tggtacgatc
     2641 tttccattgc tgttgcgccg tatgacgcga acacgatcat cgtcggtggg gtgaatatct
     2701 ggcgatcgga tgatggcggc tataattttt acctcaatgc tcattggtac ggcggaggcg
     2761 gcgcacctta cgttcatgcc gattgccacg atctcatctt cgaacccgga acatcgggct
     2821 cgtatttcgc cggctgcgac ggtggaattt ttcacaccgg gaacgacgga gcttcattcg
     2881 aagacctgag caacaatctt tgcattgcgc agatctaccg cgttggtctt tcccagcaaa
     2941 atgcgggaac gctcattacc ggccaccagg ataacggaac aaatgtgaag gtcggttcga
     3001 attatttcag cgggcttggc ggcgacggaa tggattgctt cattgaccgc acgaacgaca
     3061 acaatatgtt cggtgaattg tattacggcg atttttaccg gtccacgaat ggtggagctt
     3121 cctggaatgg aattacgaac gggctcacag gctccggcgg atgggtgaca ccctgggtgc
     3181 aggatcctgt tgacccgaac acgctctacg ccggttacga ccaggttttc aggtcgactg
     3241 acctcggctc gaactggtcg ccgacgggaa gcacgatgat cgccgcaacc gacatccagg
     3301 tagctccgtc gaatcacaat tatctttacg ttacctccgg cctcacgctt ctccgttcaa
     3361 cagatgcagg agctacgttc agcaatatta cgttcggcat caattccggc ggagccagca
     3421 tcagccgcat tgctgtctgt ccctatgacg aaaaaaagct ctgggtttcc atcagcgggt
     3481 acaacaacaa ccagaaagtt tattacagtt cagatgccgg cgcgacatgg acaaatattt
     3541 cctacggact tccgaacatt cccgccaact gcatcacggc cgttcccggc tcgatgagcg
     3601 atgcgatctt cgtcggctgc gacgtcggcg tttattatca cgataattcc atggccgggt
     3661 ggcagcctta tttcaccggg cttcccaatg ttcccgttta tgacctgaaa atatacaagc
     3721 cggcaatgtt gctgcgtgct gctacctacg gacgcggcgt ttgggaatgc cagatcgacc
     3781 agtcggttct tgttccgcag gccggttttt ctgcgaatgc aacttccgtt tgcccgggaa
     3841 caccggtgca gttcaccgat ctttcaacgt tgaacccggt agcg
//
LOCUS       DASTSF010000458         6150 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_740364,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000458 DASTSF010000000
VERSION     DASTSF010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 6150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6150
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_740364"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3023)
                     /locus_tag="VFU15_13290"
     CDS             complement(<1..3023)
                     /locus_tag="VFU15_13290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718809.1"
                     /translation="MNSFYVCSVINYQSARIQTGNTMKAFQKNLLTTVIALITVFSPA
                     FAQWNTIQHDATVNRGIHDICFVGSTGYAVGLRADSVTFGSRGLVYKTTDGGQTWNMI
                     TLPLLIGQDSVMTLNCVQFLTPMKGYAVAQCYTTDHIGGYYYGALLQTVDGGNTWTTN
                     FSTKSLVNYATGYTTNLNHVFFYSNYSAVLCGRKQTSSTNYDGVTFTTSNGGNNWYTS
                     TAFTGIQANASYFFGNATGSVVGGKMQSLAGPYNGKIVLSNDGGSTYATTFTDPGYAY
                     VDIHFPDPQTGYAVGDSMYYTTPGSSKGKIVKTTNGGLSWTSVAVFQNFMPLTVFFTS
                     ATTGFVGGETAAGNSGILKTTDGGLTWTPENYPDIATNSYISSITFSTPVTGYASNAW
                     SNSTSVYGNFMQSCGVYTGPDTAFCQLQGQLHATPATPGNNYVFSWSPGTGLSDSTAQ
                     NPFVSQVYHQQYVVTMTDTVTSCVAMDTIVVTSYNTINSPVYVCSPDSALLDFGPGAT
                     NYFWQFFTDTNNVTHTINQNTETYWATQPGQYAGYAMFTGCGALTSVVQVIDSCASSP
                     SFVCGVNAGPDTTFCQGAGQLHATPASPGNYTFSWSPANGLDDPDSQNPNIIYGVHNQ
                     QYVVTMTDTAMNCVAYDTVVVSAYYAYSDTLYACNGNYVTIDLGAGATAYTGQFTDTT
                     GNTTVITPQNEFMTVNQPGEYLIIAYYPGCGSLTSMYWLVDSCNAPIGNVWPGDCNYD
                     LTANMADALQIGLGYNTSGPARPNATNGWYAQPMADWPQNFVNCNYKHADADGNGMID
                     VNDTLPISLNYGLVHPYRLSQPVVPASSPALQLIANYDTVGLQTLVTVDVVLGTASLP
                     IDSIYGISFRISDDAGLTDTTMTLINLNTTWLGTMNGNTFGFRKYFRAAGALDVAECG
                     NDHLNRINGNGTIAQFFIVTTDNLSGIAVCHFNISDITAVTVSQNYLTLNTVNDSVVI
                     DPAHPAGIAPVKQPVVTAMYPNPANSSVTIQTNVKA"
     gene            3152..4570
                     /locus_tag="VFU15_13295"
     CDS             3152..4570
                     /locus_tag="VFU15_13295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718810.1"
                     /translation="MSARTGKISGLVRALEPAELPLFRAKLEQGRGETDKKLLRLFNA
                     VCDSVRENEDEPEKKTLFRSCFPSARYNDQKFRYLRSDLVARLERFIAQRGFERDRAC
                     FRQVLTRELASRKAAKAYNTHRLRFEEEKIASQVRDIAYYQHCYESEFIHLAKATAEM
                     KREEKSNIGEVVEHLDRFYLARKLQLCCEIFNVRNVLAVEHRVFLLDEILSHLKNKSY
                     EDTPVIRIYFRILMTLIESGNEEHFFNLRELLKEHEGKFTLPELREMYQYVLNYCIKK
                     INLGAIAWQKTLFEIYRITIENNVLMSEGYLSHWDYKNIVTISLRQKEYEWVKTFIEK
                     YRHQLLPAERENAYVYNLANLYFTTGDYSRSLKLLSKVDFSDLYYQLDSKSILLKTYF
                     ELDEADSFFYHATAFRTFLKRNRLVSDYQRSIYLNLIRYTTQLMRAAGQKKKVAIVRK
                     KVEESRNIADLNWLLSKINSME"
     gene            4757..4993
                     /locus_tag="VFU15_13300"
     CDS             4757..4993
                     /locus_tag="VFU15_13300"
                     /inference="COORDINATES: protein motif:HMM:NF021362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2061 domain-containing protein"
                     /protein_id="HEU4718811.1"
                     /translation="MKRHVAKTITWRIVGTIDTMLLGWIITGNPMTGVKIGGFEVITK
                     MLLYFFHERIWFRINFGLDHRKKKEEGTGGNLSA"
     gene            5200..>6150
                     /gene="purD"
                     /locus_tag="VFU15_13305"
     CDS             5200..>6150
                     /gene="purD"
                     /locus_tag="VFU15_13305"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014682252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEU4718812.1"
                     /translation="MNVLLLGGGGREHAFAWKLSQSKKLSKLFIAPGNAGTALHGTNV
                     NMSATDFPAVADFVLENGVKMVIVGPEDPLVKGIRDFFLADNRLGNIPVIGPPEAGAI
                     LEGSKDFSKQFMIRHGIPTAAYQTFTKETLAGGLKFLEKLSPPYVLKADGLAAGKGVV
                     IPHTLEEAKAELTAMLAEAKFGSASAKVVIEEFLKGIELSVFVLTDGVSYKILPAAKD
                     YKRIGEGDTGLNTGGMGAVSPVPFADEAFIAKVEERIVIPTIEGLKKDGTGYVGFIFI
                     GLMNDNGEPKVIEYNCRMGDPETEVVLPRIESDLLELFIAV"
BASE COUNT         1454 a         1560 c         1731 g         1405 t
ORIGIN      
        1 gccttcacat tggtctgtat tgtgacggat gagttcgccg ggttcgggta catcgcggta
       61 acgacaggct gcttcaccgg cgcgattcct gccggatgcg cggggtcgat caccacagaa
      121 tcgttcaccg tattcagcgt aagataattc tgtgaaaccg ttacggccgt gatgtcggaa
      181 atattgaaat ggcacacggc gatcccggaa agattgtctg tcgtcacaat gaagaactgc
      241 gcgatcgttc cgttaccgtt gatgcggttg aggtgatcgt tgccgcattc agcaacgtcg
      301 agcgcgcccg ctgcccggaa atatttgcgg aaaccgaacg tgttgccgtt catcgtgccg
      361 agccatgtcg tgttgaggtt gatcagcgtc atcgtcgtgt cggtgagtcc tgcgtcatcc
      421 gagatgcgga atgaaattcc gtagatgctg tcgatgggaa gcgatgcagt gccgagcaca
      481 acgtccaccg tcacgagcgt ttgcagtccc accgtgtcgt agttggcgat cagctgcagc
      541 gcaggagagc tggccggaac aacgggctgc gagagacggt aaggatgcac gagcccgtag
      601 ttgagcgaga tcggcagcgt gtcgttcaca tcgatcatcc cgttaccgtc ggcatccgca
      661 tgtttgtaat tacagttcac gaaattctgc ggccagtccg ccatcggctg cgcataccat
      721 ccgttcgtcg cattcggccg tgcgggtcct gaagtgttgt agccaagacc gatctggagc
      781 gcgtccgcca tgtttgcggt gagatcgtaa ttgcagtcgc ccggccatac attcccgatc
      841 ggtgcattgc aggaatccac cagccagtac atgctcgtca gcgatccgca accggggtag
      901 tacgcgatga tcaggtattc gcccggctgg ttcacggtca tgaattcatt ctgcggggtg
      961 atgaccgtgg tgtttcccgt cgtgtcggtg aattgtcccg tgtaagcggt tgctcccgcg
     1021 ccgagatcga tcgtcacgta gtttccgttg caggcgtaca gcgtatcgga atacgcgtag
     1081 taagcgctta ccacgaccgt gtcgtaagcg acgcagttca tcgccgtgtc ggtcatcgtt
     1141 accacgtact gctggttgtg cacaccgtaa atgatattcg ggttctgtga atccggatca
     1201 tcgaggccgt tcgccggtga ccagctgaac gtgtaattcc cgggagaagc cggtgtcgcg
     1261 tgcaactgtc ccgcgccctg gcagaacgtc gtgtcgggtc cggcatttac gccgcagaca
     1321 aatgagggag acgatgcgca ggaatcgatc acctgcacga cgctcgtgag tgcgccgcaa
     1381 cccgtgaaca tcgcgtagcc ggcgtattgc ccaggttgtg tcgcccaata ggtttctgta
     1441 ttctggttga tcgtgtgtgt gacgttattg gtgtcggtga aaaactgcca gaaataattt
     1501 gtcgcgccgg gaccgaaatc gagcagggcg gaatccggtg agcaaacata aaccgggctg
     1561 tttatcgtgt tgtaggaagt cacgacgatc gtgtccattg ccacgcaact ggtcaccgtg
     1621 tcggtcatcg tcaccacgta ttgctgatgg tacacctgtg acacgaacgg gttctgcgcg
     1681 gtggaatcgc tcagtcctgt tcccggcgac cagctgaaca cgtagttgtt gccgggagtg
     1741 gcgggcgttg cgtgcagttg tccctgcagc tggcagaagg cagtgtcggg accggtatat
     1801 acgccgcatg attgcataaa attgccgtag acggacgtgc tgttagacca ggcgttcgag
     1861 gcgtagccgg tcaccggtgt ggagaatgtg atggatgaga tgtaactgtt cgtggcgatg
     1921 tcgggataat tttccggtgt ccatgttagt ccgccgtcag tggttttaag gatgcctgaa
     1981 tttccggctg ccgtttcacc tcctacgaaa cccgtggttg cgctcgtgaa gaaaaccgtc
     2041 agcggcatga aattctggaa aacagccacg gatgtccagc tcagtccgcc gttcgtcgtt
     2101 ttcacgatct tgcctttgga agagccgggc gtcgtgtaat acattgaatc gccgacagcg
     2161 taaccggtct gcggatccgg aaaatgaata tcgacgtaag cgtagccggg atccgtgaac
     2221 gtggtcgcgt aagtgcttcc tccgtcgttc gacagaacga tcttcccgtt atacggacca
     2281 gcaagcgatt gcatttttcc gccaacgacc gaaccggttg cgttcccgaa gaaataagac
     2341 gcgttggcct gtatgcccgt aaaggcggtt gacgtgtacc agttgtttcc gccgttggac
     2401 gtggtgaagg taacgccatc gtaattcgtg gatgaagtct gttttcttcc gcagagcaca
     2461 gcgctgtaat tcgaataaaa gaaaacgtga ttgagattgg tcgtataacc ggtggcataa
     2521 ttcaccaggc tctttgtcga aaaattggtt gtccaggtat ttccgccgtc aacggtttgc
     2581 agcaaagctc cgtaatagta cccgccgata tgatcggtcg tgtagcattg tgcgactgca
     2641 tagcctttca tcggggtaag gaactgaaca cagttcagcg tcatcacgga gtcctgcccg
     2701 atcagcaggg gaagcgtgat catgttccag gtttgtccgc cgtccgttgt tttgtaaacc
     2761 agcccgcgcg atccgaacgt cacgctgtct gcgcgcagtc cgactgcata gcctgtgctt
     2821 cccacgaagc agatgtcgtg tatgcctctg ttcaccgttg catcgtgctg gattgtgttc
     2881 cattgtgcaa atgcggggga gaagacggtg atcagtgcga tcacggtcgt aaggagattt
     2941 ttttgaaacg ctttcatcgt gttgcctgtt tgaattcgtg cggattgata gttgatcacg
     3001 gaacaaacgt agaaggaatt cattggtttg aaaaatacaa aaaaacagat ttgtcggata
     3061 gatattcagg aataatttga ataatatctt acattgtcat aagtaaaatg gaacataaaa
     3121 gcgatttaca atatatattt gtcggataga tatgtctgcg cgcacaggga aaatatctgg
     3181 acttgtacgg gcgctcgaac cggccgagct gccgcttttc cgggcaaagc tggagcaggg
     3241 caggggtgaa accgacaaaa agctgctgcg cctgttcaat gccgtgtgcg attccgtgcg
     3301 tgaaaatgaa gatgagccgg aaaagaaaac gttgttccgt tcctgttttc cctcggcgag
     3361 atacaacgac cagaagttcc gctacctccg gtccgacctt gtggcacggc tggaaagatt
     3421 cattgcgcag cgcggtttcg aaagagatcg cgcgtgcttc cgccaggtgc tgacgcgtga
     3481 gctcgcttcg cggaaggctg cgaaagcgta caatacccac cgtctccgtt tcgaggaaga
     3541 aaagatcgcc tcgcaggtgc gcgatatcgc atactaccag cattgctacg aatcggaatt
     3601 cattcacctc gccaaggcga cggcggaaat gaaacgcgag gagaaaagca atatcggaga
     3661 agtggtggag cacctggacc gtttttatct tgcacgcaag ctgcagctct gctgcgaaat
     3721 tttcaatgtc cgcaacgtgc tggccgtcga gcaccgcgtt ttcctgctcg acgagatcct
     3781 gtcgcacctg aagaacaaaa gctacgagga cacgccggtc atccgcattt atttccgcat
     3841 cctcatgacg ctgatcgaaa gcgggaacga agaacatttc ttcaatctcc gcgagctgct
     3901 gaaagagcac gaagggaaat tcacattgcc ggaattgcgc gagatgtacc agtacgtgct
     3961 caattactgc atcaagaaga tcaacctcgg ggcgatcgcg tggcagaaga cgctgttcga
     4021 gatctaccgc atcacgatcg agaacaacgt gcttatgtcg gaaggctacc tgtcgcactg
     4081 ggattacaag aacatcgtca ccatcagcct gcgccagaag gaatacgaat gggtgaaaac
     4141 cttcatcgaa aaataccgcc accagctgct gcctgcggaa cgggaaaacg cgtacgtgta
     4201 caatctcgcc aacctgtatt tcaccaccgg cgattattcg cgttccctga agctgctcag
     4261 caaagtggat ttttccgatc tctattatca gctcgattcg aaatccatcc tgctcaaaac
     4321 gtatttcgag ctcgatgaag cggactcctt cttctatcac gccacggcat tccgcacgtt
     4381 ccttaaaagg aaccgtcttg tttccgatta ccagcgcagc atttatctca acctcatccg
     4441 ttacacgaca cagctcatgc gcgccgcagg acagaaaaag aaagttgcga tcgtccggaa
     4501 gaaagtggag gaaagccgta acattgccga cctgaactgg cttctttcga agatcaattc
     4561 aatggagtga tgatcggctc attgcacagc gcatcaccgg ccctgtcatc gcattgacgc
     4621 actgaacaaa tgcgaaaccc ggaaaggaga aacgcgtaca aagcggagcg gcaaacggaa
     4681 gcagctttta tttaaccggc acaccagtaa acttcaccgt atgatcatag accgattgaa
     4741 attacgagac tcgtcagtga aaaggcacgt ggcgaaaacg atcacctggc ggatcgtcgg
     4801 aacgatcgac acgatgctgc tcggctggat catcaccggc aacccgatga cgggtgtgaa
     4861 gatcggcggc ttcgaggtga tcacgaagat gctgctttat ttcttccacg aacgcatctg
     4921 gttccggatc aatttcgggc tcgatcaccg gaagaaaaaa gaagagggaa ccggcggcaa
     4981 tttatctgcg tagtcaatgt gaaaatggaa cgcagcggaa gaacaggata acggtccgct
     5041 ttcggaataa accctgtttt ttcaggtctt ccggccttca ggaaaaaatg agccgcaaag
     5101 acaacagggc cgtaagtttc accgtgaaaa tgcagactga atcatcaccc ggggtattcg
     5161 caccggttaa ttttcaggcg gacaaattac ctttgcccca tgaacgtact tctcctcggc
     5221 ggcggtggcc gcgaacatgc cttcgcatgg aaactgtctc aaagtaaaaa gctgtcgaaa
     5281 ctttttatcg cgcccggcaa tgcgggaaca gcgctgcacg gaacgaacgt gaacatgagc
     5341 gcgacggatt ttccggccgt tgcggatttt gtcctggaga acggcgtgaa gatggtgatt
     5401 gtcggcccgg aagatccgct ggtgaaaggc atccgcgatt tttttctcgc tgacaaccgc
     5461 ctcggaaata ttcccgtgat cggtccgccg gaggccggcg ccatcctcga aggcagcaag
     5521 gatttttcca agcagttcat gatccgtcat ggaattccta cagcggcata ccagacgttc
     5581 acgaaagaaa cgctggcagg cggattgaaa ttcctcgaaa agctttctcc gccgtacgtg
     5641 ctgaaagccg atggccttgc cgccgggaaa ggtgtggtta tcccgcatac gctggaagaa
     5701 gcgaaggcgg aactgacggc catgctcgcg gaagcgaagt tcggcagcgc cagcgcgaag
     5761 gtcgtcatcg aggaattcct gaaaggaata gagctttccg ttttcgtgct tacggacgga
     5821 gtttcgtata aaattcttcc cgccgcgaaa gattacaagc gcatcggtga aggcgacacg
     5881 ggcctgaaca cgggcggcat gggcgcggtt tccccggttc cgtttgctga cgaagcattt
     5941 atcgcaaaag ttgaagagcg cattgtcatc cccacgatag aagggttgaa aaaggacggc
     6001 accgggtacg tgggttttat tttcatcggc ctgatgaatg acaatggcga gccgaaagtg
     6061 atcgaataca actgccgcat gggcgatccg gaaacggaag ttgttctgcc gcggattgaa
     6121 agcgacctgc tcgaattgtt cattgccgtg
//
LOCUS       DASTSF010000459         3236 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1488609,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000459 DASTSF010000000
VERSION     DASTSF010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3236
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1488609"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..600)
                     /locus_tag="VFU15_13310"
     CDS             complement(<1..600)
                     /locus_tag="VFU15_13310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002701298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU4718813.1"
                     /translation="MTIIETIHLKKVYNETSFPVTAVNDVSLKIEKGEFTAIVGPSGS
                     GKTTLLNVIGGLDHATDGKVIIAGKDITHMSENELIDFRLHNIGFVFQAYNLVPVLTA
                     KENISFVMLLQKKTKSERERRAKELLKEVGLEDRTDNRPSELSGGQQQRVAVARALAS
                     RPAFVLADEPTANLDSKSASNLLDIMAKLNQEESMTFIFS"
     gene            complement(607..1779)
                     /locus_tag="VFU15_13315"
     CDS             complement(607..1779)
                     /locus_tag="VFU15_13315"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEU4718814.1"
                     /translation="MRSIVLLCSIVLGIWGGTFITGFMMGMMEQYVDIVIEGQLSHMQ
                     VHNPGFKKDKDIHFAIGGADELVKNISGIPGVRAASARMIVNGMATSPVTGAGVSISG
                     IDPAHEDSVTRIGDHVIDGTYFTGTKHNPVLIGEKLAKKLKVKVGGKIVLTFQDVNNN
                     ITAGAFRVTGIFHSRSSAVDASQVFVRSSDLSPLLGQQDSIHEIAILLKSNNDLLRVQ
                     AQVKKICPGLLVENWKELQPDLNVLVETMGEKTYVIITIILLALLFGITNTMLMAVLE
                     RTREIGILMAIGMNRKRVFSMVMLETIFLALIGGPLGLLFAWITIHFTGTSGIDLSAF
                     AQGLSSFGFDSTIYPTLQAGQYENIFFMVLGTSVLAAVYPSLKALRLKPADAIRKI"
     gene            complement(1825..3042)
                     /locus_tag="VFU15_13320"
     CDS             complement(1825..3042)
                     /locus_tag="VFU15_13320"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEU4718815.1"
                     /translation="MYLRLAWRNIWRNRRRTLITAASVFMAVILSVVTRSLQLGTYQR
                     MIDNVVRFYSGHIQIHQRGYNDEQTIDNSFEPNDTLKNIIAKNRFISSWAPRLESFAL
                     ASAADLTKGTLVVGVDPAPENDLTRLKAKIISGGYFDRNDKSVLVAEGLAAYLHLGVN
                     DTLVLYGQGYHGAMAAGKYPVKGILRFPSPELNNGIVYMPLPLAQELYGTGDRYTSIV
                     MLTKENVEASTVAAQLRSATGKMNFEVIGWEEMMPEMVQAIQADNSGGVIAIAVLYLI
                     ISFGIFGTVLMMLAERMHEFGILVSIGMKKLKLAFVVLLEVIMITGLGVVMGTIGGIP
                     IIAWFHSHPLRMSGDMSAYMQRFGIEPVFPFSADPSIFFSQALIVFSISALIGIYPLV
                     RLSRLNVINALKN"
     gene            complement(3050..>3236)
                     /locus_tag="VFU15_13325"
     CDS             complement(3050..>3236)
                     /locus_tag="VFU15_13325"
                     /inference="COORDINATES: protein motif:HMM:NF028441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HEU4718816.1"
                     /translation="LVDVMNASDIRMLGGKLLPGTMEMIPADKPGQKTVLVYSSLQFD
                     TPMDDNFFTVENMKNAK"
BASE COUNT          693 a          908 c          885 g          750 t
ORIGIN      
        1 tgaaaaaatg aacgtcatgc tttcttcctg gttcagcttc gccatgatgt cgagcaggtt
       61 ggacgccgat ttcgaatcga ggttggccgt aggctcgtcg gcaagcacga acgcaggacg
      121 cgaggccagc gcgcgcgcaa cggcaacacg ctgctgctgt ccgcccgaaa gctcggaggg
      181 acggttgtcg gtgcggtcct cgagcccgac ttccttcagc aattctttgg cgcgccgttc
      241 acgttctgat tttgtttttt tctgcagcag catcacgaac gaaatatttt ctttcgcggt
      301 aagcaccgga acaaggttgt acgcctggaa cacgaagccg atgttgtgga gacggaaatc
      361 gatcagctcg ttctcgctca tgtgcgtgat gtctttcccg gcaatgatca ctttcccgtc
      421 tgtcgcatga tcgagtccgc cgataacgtt cagcagcgtc gtttttcccg aacccgaagg
      481 acctacgatc gccgtgaatt cccctttctc gatcttgagg ctgacatcgt tcacagcagt
      541 aacgggaaac gatgtttcgt tgtacacttt tttcaggtgg atggtttcga tgatggtcat
      601 gacgtttcag attttccgga tcgcgtccgc cggtttcagg cgcaacgctt tcagtgaagg
      661 ataaacggcg gcaagcacgg aagtgccgag caccatgaaa aagatattct catactgtcc
      721 ggcctgcagt gtcgggtaaa tcgtgctgtc gaagccgaag gaagaaagtc cctgcgcaaa
      781 cgcggaaaga tcgatgccgg acgtgccggt gaaatggatc gtgatccatg cgaacagcag
      841 gcccagcggt ccgccgatga gcgcaaggaa aatggtttcg agcatgacca tggaaaaaac
      901 acgtttccgg ttcatgccga tcgccatcag gatgccgatc tcgcgtgtgc gttccagcac
      961 ggccatcagc atcgtgttgg tgatcccgaa cagcaaagcg agcaggatga tcgtgatgat
     1021 gacgtacgtt ttctctccca tcgtctccac gagcacgttg aggtcgggct gcagctcctt
     1081 ccagttctcg acgagaagcc ccgggcagat tttcttcacc tgcgcctgca cgcgcaaaag
     1141 atcgttgttg ctcttcagca gtatcgcgat ctcgtggatg ctgtcctgct gcccgagcag
     1201 cggtgaaaga tcggaactgc gcacgaacac ctgcgaagcg tcgacggcgg agctgcgcga
     1261 gtggaaaatc ccggttacgc ggaaggcgcc ggctgtgatg ttgttgttca catcctggaa
     1321 ggtgagcacg atctttccgc cgactttcac tttcagcttt ttcgccagct tttcaccgat
     1381 gaggaccggg ttgtgcttcg tccctgtgaa ataggtcccg tcgatgacat gatcgccgat
     1441 acgcgtcacc gaatcttcat gcgcaggatc gatcccgctg atgctcacgc ctgcgccggt
     1501 gacgggtgaa gttgccatgc cgttgacgat catccgcgcg cttgcggcac gcaccccggg
     1561 aattccgctt atgtttttta cgagctcatc cgcaccgccg atcgcgaaat gtatgtcctt
     1621 gtcttttttg aaccccgggt tgtgcacctg catgtgggaa agctgtcctt cgatgacgat
     1681 gtccacgtat tgctccatca tccccatcat gaacccggtg atgaacgtgc cgccccagat
     1741 gccgagaacg atcgagcaga ggagcacgat gcttcgcatt ttgtttcgcc aaatatttct
     1801 ccaggcaacg agcgcgagca taggttagtt ttttaatgca ttaatgacat tcagtctcga
     1861 caggcgcacc agcgggtaga tcccgatcaa tgcgctgatc gaaaaaacaa tgagcgcctg
     1921 tgaaaaaaag atggacggat cggccgagaa cgggaacacc ggctcaatgc cgaagcgctg
     1981 catgtacgct gacatgtcgc cgctcatgcg cagcgggtgc gaatggaacc aagcgataat
     2041 cggtatgccg ccgatcgtgc ccatcaccac gccgagaccg gtgatcatga tcacttcgag
     2101 cagcacgacg aacgccagct tcagcttttt catgccgatg gaaacgagga tgccgaattc
     2161 atgcatacgt tccgccagca tcatcagcac ggtaccgaag atcccgaacg agatgatcag
     2221 gtaaagcaca gcaatggcga tcacgccgcc tgaattatcc gcctgtatcg cctgcaccat
     2281 ctccggcatc atctcctccc agccgatgac ctcgaaattc atttttcccg tggcggatct
     2341 cagctgcgca gcaaccgttg acgcttccac gttttctttc gtgagcataa cgatgctcgt
     2401 gtaacggtcg ccggtgccgt acagctcctg cgcaagagga agcggcatgt agacgatccc
     2461 gttgttcagc tccggcgacg gaaagcggag aatgcctttc acggggtatt ttcccgccgc
     2521 catcgcgccg tgataaccct gcccgtacag cacgagcgta tcattcacgc cgagatgcag
     2581 gtacgcggcc agtccttcgg cgacgagaac gcttttgtcg ttcctgtcaa aatatcctcc
     2641 tgaaatgatc ttcgctttca gccgggtgag atcgttttcc ggtgcaggat caacaccgac
     2701 aacgagcgtc cctttcgtca ggtctgctgc ggatgcaagc gcgaacgact cgagccgcgg
     2761 tgcccatgaa gagatgaagc ggttcttcgc gatgatgttc ttcagcgtgt cgttcggttc
     2821 gaagctgttg tcgatcgtct gctcgtcgtt ataaccgcgc tggtgtatct gtatgtgtcc
     2881 cgaataaaaa cgcacgacat tgtcgatcat ccgctggtat gtgccgagct gcagcgaacg
     2941 cgtcactacc gaaagaatga cggccatgaa gacagaggcc gccgtgatca gcgtccgccg
     3001 tcggttcctc cagatgtttc gccatgcgag cctcaggtac atggttgcgt cattttgcgt
     3061 ttttcatgtt ctccaccgtg aaaaaattat catccatcgg tgtgtcgaat tgcagcgagg
     3121 aataaaccag cacggttttc tggccgggtt tatccgcggg gatcatctcc atcgttccgg
     3181 gcaggagctt tccgcccagc atgcggatat cggatgcgtt catgacatcc accagc
//
LOCUS       DASTSF010000460         3224 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_121062,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000460 DASTSF010000000
VERSION     DASTSF010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3224
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121062"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..816
                     /gene="gpmI"
                     /locus_tag="VFU15_13330"
     CDS             <1..816
                     /gene="gpmI"
                     /locus_tag="VFU15_13330"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013633075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HEU4718817.1"
                     /translation="DDAGNPVATLHEGDAVICFNFRTDRCREITTVLTQQDMPEHGMK
                     TIPLYYVTMTMYDHSFRNVHVIFGKDDLEKTLGEVISAAGKKQIRIAETEKYPHVTFF
                     FSGGREQEFPGEKRIMIPSPKVATYDLRPEMSAPEVTDAIVAEIEKGETDFVCLNYAN
                     ADMVGHTGVYSAVVKAIETVDACLGRVVNAGIKNGYSFIIIADHGNADYMINPDGTPN
                     TAHTTNPVPCILIDSDHKKISNGRLCDIAPTLLTLMNIPVPAEMEGKVLCGNN"
     gene            complement(1127..1417)
                     /locus_tag="VFU15_13335"
     CDS             complement(1127..1417)
                     /locus_tag="VFU15_13335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718818.1"
                     /translation="MKIILTLSIAAMISAGIYGATDMLHDVASGKMIRYNDGIPDAQK
                     KMEAEALRMNSQRQKAASRDGKMMMQAIAGRAATERPEPVRFKASEFSRGDF"
     gene            complement(1422..2096)
                     /locus_tag="VFU15_13340"
     CDS             complement(1422..2096)
                     /locus_tag="VFU15_13340"
                     /inference="COORDINATES: protein motif:HMM:NF021576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2306 domain-containing protein"
                     /protein_id="HEU4718819.1"
                     /translation="MTPRQLSIKKTVLFGLSLLFWVPVILLSLLLVQQTIPYFSFRPD
                     SNFLIERLALYAKPVWKISFYIHITAGMFCILTALLQFSSWILKKRKKIHVTAGKIYV
                     FVVLLLGAPSGFYMTFFAKGGYWERACFMLMAVFWFYSTYRGFATAAREKNYVAHKYW
                     MIRSYAMALTAVSFRVLHIVFHQYGMNDFDNYAISLWISVFGNALIAEAIVWYSAKNY
                     MKTLTA"
     gene            2402..>3224
                     /gene="queA"
                     /locus_tag="VFU15_13345"
     CDS             2402..>3224
                     /gene="queA"
                     /locus_tag="VFU15_13345"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009421022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051075 - S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEU4718820.1"
                     /translation="MKLSQFKFGLPRDLVADYPAKNRDESKLMVIHRDTGKIEHKVFK
                     DIINYFGEGDTMILNDTKVFPARLYGNKEKTGAKIEVFLLRELNRESRLWDVLVDPAR
                     KIRIGNKLYFGDDDSLVAEVIDNTTSRGRTLRFLFDGPWEEFKKTIESLGNTPIPKYI
                     RRPLQPDDAERYQTVYARVEGAVAAPTAGMHFSRELLKRLELKGVTFPMLTLHVGLGS
                     FRPVEVEDLTKHKMESEQLEIPESATEIVNEAKRNKKRICAVGTTVIRATETSVST"
BASE COUNT          818 a          864 c          853 g          689 t
ORIGIN      
        1 gacgatgcgg gaaacccggt cgccacgctt cacgaaggcg atgcggtgat ctgcttcaac
       61 ttccgcaccg accgctgccg cgagatcacc actgtgctca cgcagcagga tatgccggag
      121 cacggaatga aaaccattcc gctgtactat gtcaccatga cgatgtacga tcacagcttc
      181 cggaacgtgc atgtcatttt cggaaaggac gacctggaaa aaacgttggg tgaagtgatc
      241 tccgccgcag gcaaaaagca gatccgcatc gcggaaacag aaaaataccc gcacgtcacc
      301 tttttcttca gcggcggacg cgagcaggaa tttcccggcg aaaaacgcat catgatccct
      361 tcaccgaaag tagcgacgta cgacctccgg ccggagatga gcgcaccgga agtcacggat
      421 gcgatcgttg ctgaaatcga aaaaggggaa acggattttg tctgcctcaa ctatgcgaac
      481 gcggatatgg tcggtcacac aggcgtgtac agcgccgtgg tgaaagcgat cgaaaccgtg
      541 gatgcctgcc tcggccgcgt ggtgaatgcg ggaataaaga acggctactc attcatcatc
      601 atcgccgatc acggcaatgc ggattacatg atcaatcccg acggaacacc caacacggca
      661 cacacgacca atcccgttcc ctgcattctc atcgacagcg atcacaaaaa gatcagcaac
      721 ggaagactgt gcgacatcgc tcccaccctg ctcacgctga tgaacattcc cgtgcctgcg
      781 gagatggaag ggaaagtgct gtgcgggaac aattaattcc cggccgcctg caggtaagga
      841 tgaaccggtg gttcttcgat ccgtgcatcc gctgcgcgct tcacaaaaat tttcgcgcat
      901 tgctgcgtta accggcagaa accgccgtcg caaaaaaaga cgcccgcata cggaggaatc
      961 gtacgcggac gtgataaccg ggacatgtgg ctgcggtccc tgcctttccc ggtccgaagt
     1021 tctccttcgg tggggcaaca ttcgtagttt ccgtcgttgc cagtagtata acacgggagc
     1081 aggaaaaagt tacatcgcgg caactttttt tccgctgaag aaaagatcag aaatctcctc
     1141 ttgaaaattc ggaagctttg aaccggacag gctcgggcct ttcagtggct gctcttccgg
     1201 cgatggcctg catcatcatc tttccgtcgc gcgaagccgc tttctgccgc tgcgaattca
     1261 tccgcagcgc ttccgcttcc atttttttct gcgcatccgg gatgccgtcg ttgtaacgga
     1321 tcattttccc ggaagcaaca tcatgcagca tatcggttgc accgtaaata ccggcggaga
     1381 tcattgcggc gatcgaaaga gtgagaatga ttttcatggg ttcaggcagt taaagttttc
     1441 atgtaatttt ttgcagaata ccagacgatc gcttccgcga tgagcgcatt tccgaaaacg
     1501 cttatccaga gcgagatcgc gtagttgtcg aaatcgttca tgccgtactg gtgaaatacg
     1561 atatggagca cacggaacga aaccgcggta agcgccatgg cgtacgaacg tatcatccag
     1621 tatttgtgtg ccacataatt cttttcgcgg gcggcggtag cgaatccgcg gtacgtgctg
     1681 taaaaccaga aaacggccat gagcatgaag caggcgcgtt cccagtaacc gcctttcgcg
     1741 aagaacgtca tgtaaaatcc gctgggcgcg cccaggagca gcacgacgaa cacgtagatc
     1801 tttccagccg tcacgtggat cttttttctt tttttcagga tccacgacga gaactgcagc
     1861 agcgctgtga gaatgcagaa catcccggcg gtgatatgaa tgtagaagct gatcttccac
     1921 accggctttg cgtaaagcgc caggcgctca atgagaaagt tcgaatcggg gcggaacgag
     1981 aaatacggga tcgtctgctg cactagcaaa aggctgagca ggatgaccgg aacccagaaa
     2041 agcagggaaa gcccgaaaag aactgttttc tttatggaaa gctggcgagg ggtcatgcgg
     2101 gtgatcgtgt tcgttttctt acgtcaatat tcgttcaatg tttccgatca aaatgccctg
     2161 tccggataaa cggcggcttt tttacaataa ttggggaaac agggggcgca tctgttccgg
     2221 agccaggcgt aatcgtcctg ccggttaatt tttcgggaaa aacggcaaaa cacttgacaa
     2281 tggggtatcg ctaaggatat atttgccgcc tgttaataaa aacacaacgc cggaataatg
     2341 accttttttg cagtgcagta tcctgacgac cagcgctttc cggcaataaa ttccaatctt
     2401 gatgaaactt tcccagttta aattcgggtt gccccgtgat cttgttgctg attatccggc
     2461 caaaaaccgt gatgaatcga agctgatggt tattcaccgg gacaccggga agatcgaaca
     2521 taaggtcttc aaggatatca tcaattattt cggcgaaggg gacacgatga tcctgaacga
     2581 cacgaaagtt tttcccgcac gcctttacgg gaacaaggaa aaaacgggcg cgaagatcga
     2641 agtgttcctg ctccgtgagc tgaaccgcga aagccgcctg tgggacgtgc tcgtcgatcc
     2701 cgcacggaag atccgcatcg gcaacaagct ttacttcggc gacgacgatt cgctcgtggc
     2761 tgaagtcatc gacaatacga cctcacgcgg acgcacgctg cgtttcctgt tcgacggtcc
     2821 ttgggaagag ttcaaaaaaa cgatcgaatc gctcggcaat acgccgatcc cgaaatacat
     2881 ccgccgcccg ctgcagcccg atgatgcgga acgttaccag acggtttacg cgcgtgtcga
     2941 aggcgctgtt gccgcaccca cggcgggcat gcacttcagc cgtgaactgc tgaagcgcct
     3001 cgagctgaaa ggcgtcactt tccccatgct tacacttcat gtcgggctcg gcagcttccg
     3061 tcccgttgaa gtggaagatc tcaccaagca caaaatggaa tcggaacagc tggagattcc
     3121 cgaatccgcc accgagatcg tgaatgaggc gaagcgcaat aagaaacgca tctgcgccgt
     3181 cggcaccacc gttatccgcg ctaccgaaac atccgtttcc accg
//
LOCUS       DASTSF010000461         9497 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_267042,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000461 DASTSF010000000
VERSION     DASTSF010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 9497)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9497)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9497
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267042"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..909
                     /locus_tag="VFU15_13350"
     CDS             <1..909
                     /locus_tag="VFU15_13350"
                     /inference="COORDINATES: protein motif:HMM:NF026255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel family protein"
                     /protein_id="HEU4718821.1"
                     /translation="IDFQLGFRGEETISDGKQLANDSSFTRNYFNLFPSTFLNWAVDS
                     SHTLNFSYSRRIDRPGYGELNPFLFIVSPVMYNAGNPFLHPQISDNFELTHVYKGILS
                     VTLGYLHMTDVFMTFPHQDDSTHIFYNRTENFPTYNSASALGSVSWPVTSWFTTVTAV
                     TVFYDHYFGSLTGGGFDRSQWTWIVNSDNIITLKRNWSAEVSLFYRSLNRNGIWLEQP
                     LYGLNCGVRKNFAGGKGTVAVNFNDILWRTYVNGTAIYQDANTVVRGNFDSRRVYISF
                     SWKFGRSQYQRDTTKKSGEDEMNRAK"
     gene            complement(918..1946)
                     /locus_tag="VFU15_13355"
     CDS             complement(918..1946)
                     /locus_tag="VFU15_13355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011964381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEU4718822.1"
                     /translation="MAKKSSILLIYTGGTIGMIKDPVTGRLKPFNFERLLDEVPELRK
                     FDINIDTFSFDHPIDSSNINPGMWVSMAEIIRDNYTKYDGFVILHGSDTMAYSASALS
                     FMLENLAKPVILTGAQLPIGTIRTDGKENLITAIEIAGTKKNGKPVVPEVCIYFEFRL
                     YRGNRTNKFNAEHFRAFHSPNYPLLAEAGVNIHFNENAIAPLPRKKLSVHTKFDTRIA
                     VLKLFPGITKEVVEAYTKNKSIRAIVLETFGSGNASTERWFITSLENAIHSGKIILNI
                     TQCAAGKVQQGRYETSAELLRIGVTGGSDMTTEAATTKLMFLLGKEKDPARIRKELSA
                     SLRGELGK"
     gene            complement(1948..2721)
                     /locus_tag="VFU15_13360"
     CDS             complement(1948..2721)
                     /locus_tag="VFU15_13360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018279491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEU4718823.1"
                     /translation="MILTDTHAHLYSSKFDSDRNEMIRRSISAGVTRIFLPAIDSESH
                     AALVQLAEEWPENCFPMMGLHPTSVDPATFEQEKTLAEDWFSRKKFIAVGETGIDLYW
                     DKTHLRLQQESFAWQVELAKKLGLPVVIHSRNAFSECYEIVKERQDGSLKGVFHCFSD
                     GIAEAEKVIALGNFYMGIGGVLTYKNSGLDAVVKKFGTDPFVLETDAPYLPPVPHRGK
                     RNESAYTAIVAETMANILQRPVEEIAQATTENSKKLFGI"
     gene            2889..3497
                     /locus_tag="VFU15_13365"
     CDS             2889..3497
                     /locus_tag="VFU15_13365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718824.1"
                     /translation="MKNIVKISLAGMMLSSLAFVGCKKGPDDPFMSLHTRKGRVVGDW
                     TLKAGTETMSNGSSTTTYTWDGTNITVTQGSNSSTNGETVTVNFEKDGTYKMVTTDTY
                     TTPFAYTDTQTSTGTWNFTGKIGDDKNKDHIVMRELSNTDVVAAGSSTTTTTSSYTGD
                     SAPATVWYLDELKNKEMKITWNGTSTAGSSTDSDQGSMTFNQ"
     gene            3578..4540
                     /locus_tag="VFU15_13370"
     CDS             3578..4540
                     /locus_tag="VFU15_13370"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018477051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEU4718825.1"
                     /translation="MKKVLVIRFSSIGDIVLTTPVVRCLKAQLQAEVHYLTKENFAGI
                     VASNPNIDRVITIRKKVSEAADELKKNNYDFVVDLHHNLRSQQVKKLLKKESASFPKL
                     NSEKWLLVNARINRLPDVHIVDRYFETVKTLGVKNDGNGLDYFIPEKDEVNPGVFPAT
                     HREKFIAVTASARFGTKQLPIDKMEQIIDVLRLPVILLGGKEDSARGEELRRRCGEKV
                     FNACGMFSLNQSASVVKQAEAVIAHDTGLMHIAAAFRKKIFSVWGNTVPAFGMYPYLP
                     GEGSEMIEVKDLSCRPCSKIGFKECPKGHFRCMNDIDVKAFRRV"
     gene            4573..6423
                     /locus_tag="VFU15_13375"
     CDS             4573..6423
                     /locus_tag="VFU15_13375"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEU4718826.1"
                     /translation="MKKLTFVAAVILPALVFAQVSTPMRIPPAGVSAPPDEKKDWNPV
                     LVQTVEADEISDEADPRIKDSLAALYRGKKHPAQQAQRTATQPGAPVMGINLNGNGFN
                     NSTPNDNEVAIANNGHLISVQNSNIFRYRSSTGTVLGVQSLTSWAITLGNSATKYDPK
                     VIYDPEADRFILVCLAGYSSGSTHIMIGFSQTDSANGAYNLYQVPGNPFNDSLWSDYP
                     MISVNHNELFVTVNLLHDNMTWQAGFVQTVIWQLNKWDGYAGDTLRTELHSGINFNGR
                     NVRNLCPVEGGGNTFAGPDMYFLSERNLDAANDTVFLVHVADTANSPVQQVTVTALVS
                     NRDYFMPPNARQPGGTGNLATNDSRILGAFIQNGIIQYVSNTMDTSTGTSAVYHGMIA
                     NVSSSPSLTANMISDTSLDFGYPNIAYAGYGGPADNTAMIVFLYSDTITFPGVCAVVS
                     DGSGNYSTRTTVKAGLSYINVLSGDERWGDYSGIQRKFDELGTCWVNGMWATTNHQHA
                     TWVAQLGVSNDVGISAPAAASSAGDADVFPNPFAESVGVKFFNPQAQVIRFAVYDVNG
                     KCVDVLLQDKVNEGDVSFSFSPKPLAPGIYFMRAETADGNVLFTKKIARN"
     gene            complement(6398..7213)
                     /locus_tag="VFU15_13380"
     CDS             complement(6398..7213)
                     /locus_tag="VFU15_13380"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002759788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HEU4718827.1"
                     /translation="MDNAFWNNAGVLGQALFGYATFFLRYLVFAGGAWLFFYVLFRRK
                     VLFKRIQEEFPPRSKIRTEILYSLLTFVIFACVGVILAWFRRHGYTQLYFHLSDYPLW
                     WFFLSIPLMLLLHDAWFYWTHRFMHLKKIFPLFHKVHHLSNNPSPWASFSFHPLEAVV
                     EAGILPLIAFTLPAHPLAILIFLLMMTVMNVIGHLGYEIFPSGFTKNSITKWSNTSTH
                     HNMHHRLVKCNYGLYFNFWDRIMGTNHEKYHEQFEEVAGRKRKDDLSSGRSSS"
     gene            complement(7229..8140)
                     /locus_tag="VFU15_13385"
     CDS             complement(7229..8140)
                     /locus_tag="VFU15_13385"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEU4718828.1"
                     /translation="MGIFAPKEKSKLRSFIKKNVDEQTEKYRSDNVAILNHYKAENSR
                     LMALADSMRYAAHLQTALLPSEKDLQDIAGESFFIAVPRDTLSGDFTWFTRSGSKIII
                     AVSDCTGHGVPGALMGVLGISLLNQVVLEERCFEPSFILRRLDEKLRKTFHAKNDNQR
                     GSYDGMDISLCRIDTATGKISYAGAMRSAWIIHEEMVTELKGARYPLGGLRLEENREY
                     PSIEMTYVPGSMLYMFTDGYTDQFGGPSSPEGGKKLTRRRLRDLAVYCHRMNMREQRD
                     QFLEFFLLWKDQYEQVDDVTLLGVRLR"
     gene            complement(8274..>9497)
                     /locus_tag="VFU15_13390"
     CDS             complement(8274..>9497)
                     /locus_tag="VFU15_13390"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEU4718829.1"
                     /translation="LTFFVARRYLFSRKSHNAINIVTGISVACVAVVTASLIIILSAM
                     NGLSDLVETLYNSFHADVRITPAKGKVFIPDSMKVEELKKIPGVAWYTEIVDENALVE
                     NSDRQLIVTMRGVEDDYHEFTHFDSVVKQGRFSLHPQGRIGAVVGQEIAARMDLVELS
                     QLNLYVPKRDRNLVVDLSNMDDEPFNKEVAYVSGLYSVSSDFDGKYVILPIKNVRELM
                     DYTKECSSVEMGVSPGADKDEVLQKIKSVMGPQFLVQNRYQQNEVLFSTLKSEKMWTF
                     LILLFILFIATFNTIGSLTLLIIEKKKDIGILWSMGSDLPSIRRIFFTEGLLISFTGT
                     IAGMIIGLTLCFLQKQFGLIHLSEGFVVDAYPVAVKWMDVLLIGAAVTAIGLLAAWFP
                     VRVYTKKYQSVKFSG"
BASE COUNT         2248 a         2453 c         2587 g         2209 t
ORIGIN      
        1 atcgatttcc agctgggttt ccgcggtgaa gaaacgatct ccgatggaaa gcagctggcg
       61 aatgattctt cgttcacgcg gaattatttc aacctgttcc caagcacttt ccttaattgg
      121 gcggtcgatt ccagccacac attgaatttt tcctacagcc gccgcatcga ccggccgggt
      181 tacggggagc tgaacccgtt ccttttcatc gtgagcccgg tgatgtataa tgcgggcaac
      241 ccgttcctgc atccgcagat cagcgacaac ttcgagctga cgcacgtgta taaggggata
      301 ctcagcgtaa cgctcggcta cctgcacatg accgacgtgt tcatgacgtt cccgcaccag
      361 gatgattcca cgcatatttt ttacaaccgc acggagaatt ttccgaccta caattccgcc
      421 agcgcactcg gttctgtttc ctggccggtc acatcgtggt ttacaaccgt aaccgcggtt
      481 acggttttct acgatcatta tttcggttcg ctgacgggcg gcggtttcga ccgttcgcag
      541 tggacatgga tcgtgaattc ggacaacatc atcacgctga aaagaaactg gagcgcggaa
      601 gtgtcgctgt tttaccgttc gctgaaccgg aacgggatct ggctggaaca gccgctttac
      661 ggtcttaact gcggcgtgcg gaaaaacttt gcgggtggaa aaggaacggt tgccgtcaat
      721 ttcaacgata ttctctggcg gacgtacgtc aatggaacgg cgatctacca ggatgccaac
      781 actgttgtgc gtggaaattt cgacagccgc cgcgtgtaca tttcgttttc gtggaagttc
      841 ggaagatcgc agtaccagcg tgacaccacg aagaagagcg gggaggatga aatgaacagg
      901 gcgaaataaa gttccggcta tttgcccagt tcgccgcgaa gagaagctga cagctctttg
      961 cggatacgcg cagggtcttt ttcttttccg agcaggaaca tcagtttcgt ggtggccgct
     1021 tccgtggtca tgtcgcttcc cccggtaaca ccgatgcgca gcagctccgc gctggtttca
     1081 tagcgtccct gctgcacttt tcccgctgcg cattgcgtaa tgttgaggat gatcttcccg
     1141 ctgtgtatcg cattctcaag cgatgtaatg aaccatcttt cagtcgatgc gttgcccgag
     1201 ccgaacgtct ccagcacgat ggcgcggatg cttttgtttt tcgtgtaagc ttcgaccact
     1261 tctttcgtga tgccgggaaa aagcttcagc acggcaatgc gcgtgtcgaa tttcgtgtgc
     1321 acactcagct ttttccgggg cagcggggcg atggcattct cattgaaatg aatattcacg
     1381 cctgcttcag caagcagagg atagttgggc gaatggaaag cgcggaaatg ttctgcgttg
     1441 aacttgttcg tccggttgcc gcggtataaa cggaattcga aataaatgca cacctcgggc
     1501 acaacgggtt tcccgttctt tttcgttcct gcaatctcta tcgcggtgat gaggttttcc
     1561 tttccgtccg tgcgtatcgt gccgatggga agctgtgcgc cggtgaggat tacgggcttt
     1621 gccaggtttt cgagcatgaa gctgagcgca gaagccgaat acgccattgt atccgagccg
     1681 tggagaatga caaacccgtc gtatttcgtg taattatcgc ggataatttc cgccatgctc
     1741 acccacatgc cggggtttat gttggatgaa tcgatcgggt gatcgaagga aaacgtatcg
     1801 atgttgatgt cgaatttccg cagttcggga acttcgtcga gcaggcgctc gaagttgaac
     1861 ggcttcagtc ttcccgttac gggatctttg atcatcccga tcgtgccgcc ggtgtaaatg
     1921 aggaggatgg aggatttttt cgccatgtca gatgccaaaa agcttttttg agttttcagt
     1981 ggtggcctgc gcgatctctt ccaccggccg ctgcaggatg ttcgccatgg tttccgcgac
     2041 gatcgcagtg tacgcgcttt cattgcgttt cccgcgatgc ggaacaggag gaaggtaagg
     2101 cgcatccgtt tccagcacga acgggtctgt cccgaatttt ttcaccaccg catcgagccc
     2161 ggaatttttg taggtgagca caccgcctat gcccatgtaa aaattgccga gcgcgatcac
     2221 tttttccgct tccgcgatgc catcggaaaa gcagtgaaaa acgcctttca gcgaaccgtc
     2281 ctgccgttct ttcacgatct cgtaacattc gctgaaagca ttgcgcgaat gaatgacgac
     2341 ggggagaccg agttttttag caagctccac ctgccaggca aaagattcct gctggagacg
     2401 gagatgtgtt ttgtcccagt aaagatcgat gcccgtctca cccacggcga tgaatttttt
     2461 tcgcgagaac cagtcttccg ccagcgtttt ttcctgctca aacgtagccg gatcgacgga
     2521 agtgggatga aggcccatca tcgggaaaca gttttccggc cattcttccg ccagttgaac
     2581 cagcgcagcg tggctttccg aatcgatggc gggcaggaaa attctcgtta cgccggcact
     2641 gatcgatctc cggatcatct cgttacggtc gctgtcgaat ttcgaagaat ataaatgcgc
     2701 gtgggtatcg gtgaggatca tcgcgcaaag atacttattg gatacggcgc agcaccgcac
     2761 gcggttttac aatcccttta ctgcgaattt gcataatatt gatagtcatt gctttatgct
     2821 ttttttgttt ttgcgaatac ttgcctacat ttgcaccgct aaaaaccatt aaacccttct
     2881 aaaacaacat gaaaaacatt gtaaaaattt ctctcgccgg gatgatgctc tcttcccttg
     2941 cattcgtagg atgtaaaaaa ggaccggatg atccgttcat gtcactgcac acgcgcaaag
     3001 gccgtgttgt tggcgactgg accctgaaag caggtacgga aaccatgtcc aacggaagct
     3061 ctactacgac ctacacctgg gatggtacga acattacggt tacgcaaggc agcaactcca
     3121 gcaccaacgg agagacggtt acggtaaact ttgagaagga cggcacctac aaaatggtga
     3181 ctaccgatac ctacacgacc ccgtttgcct acacggacac gcaaacgtct accggtacct
     3241 ggaacttcac gggcaagatc ggcgacgaca agaacaaaga tcacatcgtt atgcgtgagc
     3301 tttccaacac ggatgttgtg gctgccggct cttccaccac cacgaccaca tcgtcgtaca
     3361 ccggcgacag cgctcccgct acggtatggt acctcgacga gctgaagaac aaagaaatga
     3421 agatcacctg gaacggtacg agcaccgccg gaagctcaac agacagcgac cagggttcta
     3481 tgaccttcaa ccagtaatcg gttaatgctt ttccggaaag ccgctgtgta cagcggcttt
     3541 ctttttttat ggacattctc ttcttatttt tcggcgggtg aaaaaagtgc tcgtcatacg
     3601 tttcagctcc atcggcgaca tcgtgctcac gacgccggtg gtacgctgcc tgaaggcgca
     3661 gctgcaggcg gaggttcatt acctgacgaa ggaaaatttt gcgggtatcg tcgcttccaa
     3721 tccgaatatc gaccgcgtga tcacgatccg gaaaaaagtt tccgaagcgg cggacgagct
     3781 gaaaaaaaac aactacgatt ttgtcgtcga cctgcaccac aacctgcgtt cgcagcaggt
     3841 gaagaagctg ctgaaaaaag aatctgcatc ctttcccaag ctgaacagcg aaaaatggct
     3901 gctcgtcaat gcgcggatca accgtttgcc ggatgttcac atcgtcgacc gctatttcga
     3961 aacggtgaaa acgctgggcg tgaagaacga cggtaacggt cttgattatt ttatccccga
     4021 aaaggacgaa gtaaatcccg gtgtctttcc cgcaacgcac cgcgaaaaat tcattgcggt
     4081 caccgccagc gccaggttcg gaacgaagca gcttcccatt gacaaaatgg aacagatcat
     4141 cgatgtgctt cgtctgccgg tcatcctgct tggcggaaaa gaagactcgg cgaggggaga
     4201 agagctccgg agacgctgcg gggaaaaagt gttcaacgca tgcgggatgt tcagcctgaa
     4261 ccagtcggcc tcggttgtga agcaggcgga agcggtgatc gcgcacgaca cgggactcat
     4321 gcacattgcc gcggcgttcc gtaaaaaaat attttccgtt tggggaaaca cggttccggc
     4381 tttcggaatg tacccgtacc tgccgggtga aggttcggaa atgatcgaag tgaaagatct
     4441 ttcctgccgg ccctgttcca agatcggttt taaagaatgc ccgaaaggac atttccgctg
     4501 catgaacgat atcgacgtga aggcgttccg cagggtctga aaaaaggcgg aaaatgccta
     4561 acttgtaata ctatgaagaa actaactttt gttgctgcag ttattcttcc ggctttggtc
     4621 ttcgcgcagg tgagcacacc gatgcgcatt ccgcccgcag gcgtttcagc acccccggat
     4681 gaaaaaaaag actggaaccc cgtgctcgtg cagacggtgg aagccgacga aatttctgac
     4741 gaagcggatc cgcgcatcaa ggacagtctt gccgcgcttt accgcgggaa aaaacatcct
     4801 gcgcagcagg cgcagcgcac ggccacgcaa cccggcgcac cggtgatggg gatcaacctg
     4861 aacgggaacg ggttcaacaa cagcacaccg aacgacaatg aagtggcgat cgccaacaac
     4921 ggccatctta tctccgtgca gaacagcaat attttccgtt accggtcgtc aacgggaacc
     4981 gtgcttggcg tgcagtcgct caccagctgg gccatcacgc tcggcaacag cgcgacgaag
     5041 tacgatccga aagtgatcta cgatcccgaa gccgaccgct tcatcctcgt gtgccttgcg
     5101 gggtattctt ccggcagcac gcacatcatg atcgggtttt cgcaaacgga ttccgcgaat
     5161 ggcgcgtaca acctgtacca ggttccgggc aacccgttca acgattcgct gtggagcgat
     5221 tacccgatga tctctgtcaa tcacaacgag cttttcgtga cggtgaatct cctgcatgat
     5281 aacatgacgt ggcaggcggg gttcgtgcag accgtgatct ggcagctgaa caaatgggac
     5341 ggctacgccg gtgatacgtt gcggactgaa ctgcacagcg gcatcaattt caacgggagg
     5401 aatgtgcgca atctgtgtcc tgttgagggt ggcggaaata ctttcgccgg gccggacatg
     5461 tattttcttt ccgaacgcaa cctcgatgcg gcgaacgaca ccgtttttct cgtgcacgtg
     5521 gcggatacgg cgaactcacc ggtccagcag gtgacagtaa ccgcgctggt cagtaaccgc
     5581 gattacttta tgccgccgaa tgcccgccag ccgggcggta ctgggaacct ggcgacgaat
     5641 gattcgcgta tcctcggcgc atttatacag aacggcatca tccagtatgt gagcaacacg
     5701 atggacacgt cgacggggac aagcgccgtt tatcatggca tgatcgcgaa cgtaagctct
     5761 tcaccttcgc tgactgcgaa tatgatctcc gacacatcgc tcgatttcgg ctacccgaat
     5821 attgcctatg ccggttatgg cggacccgca gacaatacgg cgatgatcgt tttcctttat
     5881 tcggacacga tcacatttcc cggcgtgtgc gctgtcgttt cggacggaag cgggaattat
     5941 tccacgcgca cgaccgtgaa agcgggattg tcctacatca atgtgctttc cggggatgaa
     6001 cgatggggcg attattcggg catacagcgg aaattcgatg agctcggcac ctgctgggtg
     6061 aacgggatgt gggccacgac caatcaccag cacgcaacgt gggtggcgca gctcggtgtt
     6121 tcgaatgacg tgggcatcag tgcgccggca gctgcttctt ctgcgggcga tgcagacgtt
     6181 tttcccaatc cctttgcgga atcggtgggt gtgaaattct tcaacccgca ggcacaggtg
     6241 atccgtttcg ccgtgtatga cgtgaacgga aaatgtgtcg atgtgctttt gcaggataaa
     6301 gtgaacgaag gcgatgtgag cttttcgttc tcaccgaagc cgctcgctcc cggaatttat
     6361 ttcatgcgtg cggaaacggc ggacgggaat gttctgttca cgaagaagat cgcccggaac
     6421 tgaggtcatc ttttcttttt cttcccgcca cctcttcgaa ctgctcatgg tatttttcat
     6481 gattggttcc catgatcctg tcccagaaat taaagtaaag cccgtaattg catttcacga
     6541 ggcggtgatg catgttgtga tgcgttgacg tattgctcca tttcgtaatg gagttcttcg
     6601 taaagccgga agggaatatt tcatatccca ggtgcccgat cacattcatg accgtcatca
     6661 tcaaaagaaa aataagaatg gcaagcgggt gcgccggcag ggtgaacgcg atgagcggaa
     6721 ggattcctgc ttcgaccacg gcttccagcg ggtgaaacga gaacgaggcc cagggagaag
     6781 ggttgttgga aaggtgatgc actttgtgaa aaagcgggaa gatctttttc aggtgcatga
     6841 agcggtgcgt ccagtagaac catgcatcgt gcagcagcag catgagcggg atgctcagga
     6901 aaaaccacca gagcgggtaa tcgcttaaat ggaaatacag ctgcgtgtag ccgtgcctgc
     6961 ggaaccaggc gaggatcaca ccgacgcacg cgaaaatgac aaacgtgagc agggaataaa
     7021 ggatctccgt ccggatcttc gaacgcggcg ggaattcttc ctgtatccgt ttgaacaaaa
     7081 ccttcctgcg gaaaagaaca tagaaaaaca gccaggctcc tcctgcaaaa acaagatagc
     7141 gcaggaagaa agtcgcgtaa ccgaacagcg cctgcccgag aacgcctgca ttgttccaga
     7201 aagcattatc catgatgaaa accggtaatt acctcagccg gactcccagc aaagttacat
     7261 catctacctg ctcgtattgg tctttccaga ggagaaaaaa ctccaggaac tggtcgcgct
     7321 gctcgcgcat gttcatgcgg tggcaataga cggcaaggtc gcggaggcgg cggcgtgtca
     7381 gcttttttcc gccttcgggc gaggaaggtc cgccgaactg atcggtgtag ccatccgtga
     7441 acatgtacag catcgagccc ggcacgtacg tcatttctat ggaaggatat tcccggttct
     7501 cttcgaggcg gaggccgccg agcgggtaac gcgcgccttt cagctctgtc accatttcct
     7561 cgtggatgat ccacgcggaa cgcatggcgc cggcatagga aatttttccc gtggccgtat
     7621 cgatgcggca gagggagatg tccattccgt cgtacgatcc gcgctggttg tcgttcttcg
     7681 cgtgaaaagt tttgcgcagc ttttcatcca gccggcgcag gatgaaggaa ggctcgaagc
     7741 atctttcttc cagcacgacc tggttgagca ggctgatacc gagaacgccc atgagcgcgc
     7801 ccggaacgcc gtgaccggta caatccgaaa cagcgatgat gatcttcgat cccgatcgtg
     7861 tgaaccaggt aaaatcgccg ctcagcgtat cgcggggaac ggcgatgaaa aatgattcgc
     7921 ccgcgatatc ctgcaggtct ttttcagaag gcagcaatgc ggtctggagg tgcgcggcat
     7981 aacgcatgct gtcggcgagc gccatgagcc gggaattttc cgctttgtaa tggttgagga
     8041 tggcgacgtt gtcggagcgg tacttttcag tttgttcgtc aacgttcttt ttgatgaagg
     8101 aacggagctt cgatttttct ttcggggcga atattcccat agagagataa ctgaaaagat
     8161 tgaaggacac gcccaaaatt acttcccggc tgtgtctgct tctttaagca aacattaact
     8221 ccagtcaaga tgatttcgga gaaattaaat tatccgtcta tgaacaggga aattcagccg
     8281 ctgaatttta cggactggta ttttttggtg taaacgcgta ccgggaacca tgctgcgaga
     8341 agcccgatcg ccgtcactgc tgcgccgatg agaagaacgt ccatccattt tactgcgaca
     8401 ggataagcgt cgacgacaaa accttcgctg aggtgaatga gcccgaattg cttctgcaaa
     8461 aaacaaagcg taagcccgat gatcatgccg gcaatggtcc cggtgaagga aatgagcaaa
     8521 ccttccgtga aaaagatgcg gcggatggaa ggaagatcgg agcccatgct ccagagaatg
     8581 ccgatgtctt ttttcttttc gatgatgagc agcgtgagcg aaccgatcgt gttgaatgtg
     8641 gcgatgaaaa gaatgaagag aagaatgagg aacgtccaca tcttttccga tttgagcgtg
     8701 ctgaaaagca cttcgttctg ctggtaacgg ttctgcacca ggaattgcgg acccatgacg
     8761 gattttattt tctgcagcac ctcgtccttg tccgcgccgg gactgacacc catttccacg
     8821 ctgctgcatt ctttcgtata gtccatcagc tcgcgcacgt tctttatcgg aaggatgacg
     8881 tatttcccgt cgaaatcgct gctgacggaa taaagcccgg aaacgtatgc gacttccttg
     8941 ttgaacggct cgtcgtccat gttgctgaga tcgaccacga ggttgcggtc gcgtttcgga
     9001 acgtaaaggt tgagctgcga aagctcgaca aggtccatgc gtgcggcaat ttcctgtccc
     9061 accaccgcgc cgatccttcc ctgcgggtgg agcgaaaacc gtccctgctt cacgacggaa
     9121 tcgaaatgcg tgaactcgtg gtaatcgtct tccacgccgc gcatcgtgac gatcagctgg
     9181 cgatcgctgt tctcgaccag cgcgttctcg tcaacgatct ccgtatacca ggccacgccc
     9241 gggatttttt tcagctcttc caccttcatg gaatcgggaa tgaacacttt tcctttcgca
     9301 ggcgttatgc ggacgtcggc gtgaaacgaa ttgtacagcg tttccacgag atcggaaaga
     9361 ccgttcatgg cggaaagaat gatgatgagc gaagcggtga caacggccac gcaggcgacc
     9421 gatatcccgg taacgatatt gatcgcgttg tgcgatttgc gtgagaaaag gtaacgccgc
     9481 gctacaaaga aggtcag
//
LOCUS       DASTSF010000462         5011 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1693962,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000462 DASTSF010000000
VERSION     DASTSF010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5011
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1693962"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..391)
                     /locus_tag="VFU15_13395"
     CDS             complement(<1..391)
                     /locus_tag="VFU15_13395"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEU4718830.1"
                     /translation="MKSLPDTVRGGIYEALFNKAAEGVIAIDRQQDIRLVNPCAERLL
                     GYSGDDLKEKKLSDIFEPAARREMMQWYENIFNDRRTDPGGRTVTTLLKKDGTGLPVE
                     IDAYNHLLTEEGPFLIALFCDIRERRQA"
     gene            complement(502..4236)
                     /locus_tag="VFU15_13400"
     CDS             complement(502..4236)
                     /locus_tag="VFU15_13400"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEU4718831.1"
                     /translation="MQFFPSLQRLWSARYTKLALAVLMFAAAYLLSLSVSGVRTSTGI
                     PAFERTLHEKEKMLNAEVDSLVAFSAGKNYDRIFTERPERYDALFREKGIVLLIYESD
                     TLKFWSDNSVAVENFMKEVCLDDRMAKLKNGWFEICRKTDDKGRTFIGLLLIRSEYSY
                     QNQYLVNDFQRDFNVPAETYLAIDAPNSANSIHDSSGNYLFSLVFKSTGPPESAQWIT
                     VFNLLLNLSGFLFMLLFLRSESDLLSAHTGKKWAPVLFGGSVLLLRFLAIRTGFPHAF
                     YDLTIFGPKFYGDADSFWNANLGDFLINAFILLYLAAYMTRFLREWKFTALKTTWKKL
                     AAGTALFLPVSLAAWAVNSMISGLVKNSDISFNVNNLFSLSGYSYCGLLLVALLLASF
                     FLITDRFIKMMRVLVPENSLRWYAFIAAGLLHTLILHLSGSVDLIAVLWPYAIFFILL
                     NWNTDNDSAYSFSLIVLLVFIFAFYATHMLLKQSTNKEHDSRKVFAEKLAAEQDPIAE
                     HLFTEFESKLTYDTVLINYTRPPFQDFSAFEKRVQQQYFSGYWDKYEVKVTFFDTMCT
                     PVIRSSSPYSDNILHFEEMEENQGSPTFSNNFFYLDNNSGRISYLAKVVMQEPGREKP
                     LGQGTLFIELDSKLMSEEIGFPELLLDRELGINQKLVNYSYAKYKNGKLVNHSGKFQY
                     NLTPDIYAPYKQHDQFLEGNGWCHLIYRPDNATVVILSMPEEGWLDMTTSFSYIFALF
                     SLLLLVSLSVRQLVLVGPTFSNLSFRYRIQLVLVMIVLVSLGLFGGGSIYYIRQQYQA
                     KNTEIISEKGRSVVLELENKLEDTKLTPTYRDYATYLLKKFSNVFFTDINLFDVHGNL
                     YASSRPKVFEEGLVSSKMNPEAYREMRLEEKSEFVHDENIGNLNYLSAYLPVKSQDGQ
                     ILAYLNIPYFAKQSDLEKEISTFLVALINVYVLLFALSVLAAIFISNYLTHPLRLIQE
                     KMRQVKLGRQSDQIEWKNKDEIGSLVSEYNRMISELAHSAELLAQSERESAWREMAKQ
                     VAHEIKNPLTPMRLSIQLLERAYRDKAPDIDQKVERLSKTMLEQIDTLSSIASAFSDF
                     AKMPKPNNEGMDLKALVQNAIDLFHETSETTDFHFDSNGMETAWICADKEQLRRVFNN
                     LFRNGIQAIPEGRKGRIDVTLSRQGKNFIVSVKDNGSGIADEVIDKIFVPNFTTKTAG
                     MGLGLAMVKNIVESCSGTIWFETTREKGTTFYVSFPEFEE"
     gene            4336..>5011
                     /locus_tag="VFU15_13405"
     CDS             4336..>5011
                     /locus_tag="VFU15_13405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718832.1"
                     /translation="MAKRAAPKKVSARRKAVVFSVPEIRGRGWFFTNDFRIQGLVIVI
                     AGLLFYWNSWNNQYALDDDIIMNENMYVQKGFSGIPEILSNDAYKSYYQSMGVEQQLN
                     GGRYRPLSIVSFAIEQQIFGDCYGDRYSDVRDSLLDLQKKGITDAPYMNRLINERNAL
                     DTKIKDSNLKIAPVRHIFQILWFVLSMVVLLYFLREFIFRTNTDVAFLAVLLFTIHPI
                     HTEVVAN"
BASE COUNT         1098 a         1337 c         1383 g         1193 t
ORIGIN      
        1 ccgcctgcct gcgttccctg atgtcgcaga acaacgcgat caggaacggc ccttcctccg
       61 taaggagatg gttataggca tcaatctcga ccggcaaacc ggtgccgtct tttttcagca
      121 gggtggtaac ggttcttcct cccgggtccg ttctccggtc attgaaaata ttctcgtacc
      181 actgcatcat ttctctcctt gcagccggtt cgaaaatatc gctcagcttc ttttctttca
      241 gatcatctcc cgaatacccg agaagtcgtt cagcgcaggg attcacaaga cggatgtcct
      301 gttgcctgtc gatagctatt acgccctccg ctgctttatt gaacagggct tcgtaaatgc
      361 cgccccttac ggtatctggt aaacttttca tttgcgggtc aattgatttt acaaaaaagg
      421 cctgcaaata aaaacgtggg gatgacaatg gtcgcctcag aatatgactt ccgtcaccaa
      481 cccgggcaat gaaacggggt tttattcttc aaactccgga aagcttacat aaaacgtcgt
      541 ccctttttcc cttgtcgtct cgaaccagat cgtaccgctg cagctttcga cgatgttctt
      601 caccatggcg agaccgagtc ccatgccggc ggttttcgtg gtgaaattcg gcacgaagat
      661 tttatcgatc acttcatcgg cgatgccgct tccgttgtct ttcaccgaga cgatgaaatt
      721 ttttccctgc cggctgagtg ttacgtctat tcttcctttg cggccttcgg gaatggcctg
      781 tatgccgttg cggaaaagat tgttgaacac gcggcgcagc tgttccttgt cggcgcagat
      841 ccatgcggtt tccatgccgt tgctgtcgaa atggaaatcc gtcgtttcgc tcgtctcgtg
      901 gaacaggtcg atcgcgtttt gcaccagcgc tttcaggtcc atgccttcgt tgttcggctt
      961 cggcattttt gcgaaatcgg agaatgcgct cgcgatggaa gaaagcgtgt cgatctgctc
     1021 gagcatcgtt ttcgaaaggc gttccacttt ctggtcgatg tcgggtgcct tgtcgcggta
     1081 agcgcgttcg agcagctgga tgctcagccg catcggcgtg agcgggttct tgatctcgtg
     1141 cgccacctgc ttggccattt cacgccacgc gctctcgcgt tccgactgcg cgagcagctc
     1201 cgcactgtgc gccagctcgc tgatcatgcg gttgtattcg ctcacgaggc tgccgatctc
     1261 gtctttgttc ttccactcga tctggtccga ttgccgcccg agcttcacct gccgcatttt
     1321 ttcctggatg agccggagcg ggtgcgtgag gtaattcgaa atgaaaatcg cggccagcac
     1381 cgacaatgcg aacagcagca cgtacacgtt gatcagcgca acgaggaacg tggaaatttc
     1441 cttttcaaga tcgctctgct tcgcgaaata cgggatgttc aggtacgcga ggatctgccc
     1501 gtcctggctt ttcaccggca ggtaggccga gaggtagttg aggttgccga tgttctcgtc
     1561 gtgcacgaat tcggattttt cctcgagacg catctcgcgg tacgcttccg ggttcatctt
     1621 ggaggaaacg agtccttctt cgaacacttt cggccgcgac gatgcgtaaa gattgccgtg
     1681 cacatcgaag aggttgatgt cggtgaaaaa gacgttggag aattttttca gcaggtacgt
     1741 cgcataatcg cggtacgtgg gcgtgagctt cgtatcctcc agcttgttct ccagctcgag
     1801 caccaccgat cttccttttt cgctgatgat ctccgtgttc ttcgcctggt attgctggcg
     1861 gatgtaatag atcgagccgc cgccgaaaag tccgagcgaa accagcacga tcatcacgag
     1921 cacgagctgt atgcggtaac ggaagctgag gttcgagaac gtcggtccga cgagcacgag
     1981 ctggcgcacg gaaagtgaaa cgagcagtag gaggctgaac agcgcgaaaa tgtacgagaa
     2041 gctcgtcgtc atgtcgagcc agccttcttc gggcatgctg aggatgacga cagtcgcgtt
     2101 atcgggacga taaatgagat ggcaccaccc gttgccctcg aggaactggt cgtgctgctt
     2161 gtacggtgcg taaatatccg gcgtgaggtt gtactggaat ttcccggaat ggttgaccag
     2221 tttcccgttc ttgtatttcg cgtacgaata attgacgagc ttctggttga tgcccagctc
     2281 gcggtcgagc agaagttccg ggaaaccgat ctcttccgac atcagctttg aatcgagctc
     2341 gatgaacagc gttccctgcc ccagcggttt ttccctgccg ggctcctgca tcacgacttt
     2401 cgcgagatag gaaatgcggc cggaattatt gtcgaggtaa aagaagttgt tgctgaacgt
     2461 cggcgagccc tggttctctt ccatctcctc gaaatgcagg atgttgtcgg agtacggcga
     2521 tgacgagcgg atcaccggtg tgcacatggt gtcgaagaac gtcaccttca cttcgtattt
     2581 gtcccagtag ccgctgaaat attgctgctg cactcttttt tcgaacgcgc tgaaatcctg
     2641 gaacggcgga cgcgtgtaat tgatcagcac ggtgtcgtac gtgagcttgc tttcgaattc
     2701 ggtgaaaaga tgttcagcga tcgggtcctg ctccgccgcc agtttttcgg cgaacacctt
     2761 gcggctgtca tgctccttgt tcgtgctctg cttgagcagc atgtgggtgg cgtagaacgc
     2821 gaagatgaaa acgagcagca cgatgagcga gaacgaatac gcgctgtcgt tgtccgtgtt
     2881 ccagttgagc aggatgaaga aaatggcgta cggccacagc acggcgatca ggtcgaccga
     2941 accggaaaga tgaaggatga gcgtgtgcag caatcccgcc gcaatgaacg cgtaccaccg
     3001 cagtgaattc tccggcacga gcacgcgcat cattttgatg aagcggtcgg tgataaggaa
     3061 gaacgaggcg agcagcagcg caacgagcag caatccgcag tagctgtacc cggaaagact
     3121 gaacaggtta ttgacgttga acgaaatatc cgagttcttc accagtcccg agatcatgct
     3181 gttcacggcc cacgcggcga gggaaacagg caggaaaagc gccgtgccgg ccgcgagctt
     3241 tttccatgtg gtcttcaatg cggtgaattt ccattcccgg agaaaacgcg tcatgtacgc
     3301 cgcgaggtaa agcaggatga atgcgttgat gagaaaatcg ccgaggttcg cgttccagaa
     3361 tgaatcagcg tcgccgtaga actttgggcc gaagatcgtc aggtcgtaga acgcatgcgg
     3421 gaacccggta cggatcgcca ggaaacgcag gagaagaacg gagccgccga aaagcacggg
     3481 tgcccatttt ttccccgtgt gcgccgacag caggtcgctc tcgcttcgca ggaacagcag
     3541 catgaaaaga aaccccgaca ggttgagcag gagattgaac acggtgatcc actgcgcgct
     3601 ttccggcgga cctgtcgact tgaacacgag ggaaaaaaga taattgccgg aggaatcatg
     3661 aatgctgttc gcggaattcg gcgcgtcgat cgcgaggtac gtttccgcgg gaacgttgaa
     3721 gtcgcgctgg aaatcattga cgagatactg gttctggtag gaatactcgc tgcggataag
     3781 cagcagcccg atgaacgtgc gtcctttatc gtcggtcttg cggcagatct cgaaccagcc
     3841 gttcttgagc ttcgccatgc ggtcgtcgag gcagacttcc ttcatgaaat tttccacggc
     3901 gacggaattg tccgaccaga atttcagcgt atcgctttcg taaatgagca gcacgatccc
     3961 tttttcgcgg aaaagcgcat cgtaacgttc gggacgttcg gtgaagatgc ggtcgtaatt
     4021 tttaccggct gagaacgcca cgagcgaatc gacttccgcg ttgagcattt tttccttttc
     4081 gtgcagcgtg cgctcgaatg caggaatgcc cgtggatgtg cgtacgccgg aaacggaaag
     4141 ggacagcagg tacgccgccg cgaacatcag caccgccagc gcaagcttcg tataccgcgc
     4201 cgaccaaagc ctttgaaggg aagggaaaaa ctgcatccgt acaaatgtaa gcaagaaggg
     4261 gtgccgcctt tgcatcaaaa accttaccac gtcctgcatt ttcacacgtc ccaatttctt
     4321 agtttcgtac ttcctatggc aaaacgcgct gcgcctaaaa aagtttccgc ccggagaaaa
     4381 gccgttgtct tttccgttcc cgagatcagg gggcgcggct ggttcttcac caatgatttc
     4441 cggatacagg gactcgttat cgttattgcc ggactgctgt tctactggaa ttcctggaac
     4501 aaccagtatg cgctcgacga tgacatcatc atgaacgaga acatgtacgt gcagaaagga
     4561 ttttccggca tccccgagat cctttccaac gacgcgtaca aaagctatta ccagagcatg
     4621 ggcgtggagc agcagctcaa cggcggacgt taccgcccgc tctcgatcgt ttccttcgcc
     4681 atcgaacagc agatcttcgg cgattgttac ggcgaccgct attccgacgt gcgcgattcg
     4741 ctcctcgatc tccagaaaaa aggcatcacc gatgcgccgt acatgaaccg gctcatcaac
     4801 gagcggaatg cgctggacac gaagatcaag gattccaatc tgaaaattgc gccggtccgg
     4861 cacattttcc agatcctctg gttcgtgctg tcgatggtgg tgctgctcta tttcctgcgc
     4921 gaattcattt tccgcaccaa cacggatgtc gcgttcctcg ccgtgctgct gttcaccatt
     4981 cacccgatcc acacggaagt ggtggcgaac g
//
LOCUS       DASTSF010000463         3467 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1780106,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000463 DASTSF010000000
VERSION     DASTSF010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3467)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3467
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1780106"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1036
                     /locus_tag="VFU15_13410"
     CDS             <1..1036
                     /locus_tag="VFU15_13410"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU4718833.1"
                     /translation="VQMNHLYDDDLWQKRCLYEYQRYEDAYFKDLLYVYKKRQNNPNA
                     YVNDSKLKSGACFSSMLLQCTLATLYCSKQEIASLILHEYVIAPKTDTTISDDMQDQF
                     NKAGQDYDNSNYGDARRQYEKLVRKDSTYFKATNRVGLCYYKTEDYDNAIIWFKKAIA
                     LQPLMIEPRVSLVRCYKEEHKWDDAYNACIDGIIVYPDDRLFMLLDETCDKLGKTFDR
                     HFMPRDYIPNMANNDDQGSIDESPWSFYRMAKDKIMDYCDKNGVITRTNDVTKQGYLE
                     SFSWEYMLQKSTPEGKEFPFAKKMMNEGYIDCYSMVSLFHITMWDQYHAFSQANADRI
                     RTYITTYLVK"
     gene            complement(1105..3054)
                     /locus_tag="VFU15_13415"
     CDS             complement(1105..3054)
                     /locus_tag="VFU15_13415"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEU4718834.1"
                     /translation="MIKKYVSKFRILVVAGVAALLIALVTGLGGQLAEARPYHSKRDL
                     ARIHEFFQSQPYDTNTYFATGGRCGGCHGHDPTHISLYTSWGEDVNIMDDWQGTMMAN
                     SAKDPLWRAKVSHETAVNPGLQLETEDKCTTCHAPTGHFEAHYLGQQYYTMNMLATDS
                     FGLDGVNCTGCHEIKDTLQGNNFTGNLFYAQKQVFGPVQDPVSAVMEFFVGFTPSYSA
                     HMTHSELCAGCHTLITNTTDLSGNLTGGTYIEQATYHEWKNSSYPANGITCQHCHIPQ
                     IQDSVKLATNYPFIEGRFPFDRHHLIGGNAFMLKLMQGNMSTVGNTCESWNFDTTINR
                     TTRYLRDSTLNMQVVQTARTVDTVFYDVQLTNKCGHKFPSGYPARIAWVEFVVTDNLG
                     DTLFADGLHDAAGNVIGRDPGFEPHHDVINNVNDVQIYEMVMGDVNNNPTTVLERADT
                     CLKDNRIVPVGFSTSHPSYDSTRVIGVTNDPDFNYNGPVEGTGGDIVHFHVFLNGYAG
                     ALNVTSRVYYSSVPAKWLTEMFSYNTAPINSFHTMYDNADHTPMLVAEQIMTNTITGT
                     SSQNGLPGITCYPNPVTDGTVHFSGYAGSGLQEVRVYDLSGKEVLPAIPGNEFDGTLV
                     LPGRGVYLVSFRTARGTVVKKIISH"
     gene            3172..>3467
                     /locus_tag="VFU15_13420"
     CDS             3172..>3467
                     /locus_tag="VFU15_13420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718835.1"
                     /translation="MTKHLPKFILALAASLPVAAFAQTTITPFSDQTSLLPSSVFHSG
                     NAVGVCDMNGDFKDDIVRDSMNKQMYIEFQQAPNGTFAQTAYNTGSFGSPWGLC"
BASE COUNT          842 a          928 c          885 g          812 t
ORIGIN      
        1 ggtgcagatg aatcacctgt atgacgatga tctctggcag aaacgctgcc tgtatgaata
       61 ccagcgctac gaggatgcct acttcaagga cctgctttat gtttacaaaa aacggcagaa
      121 caatcccaat gcctacgtca acgattcaaa gctgaaatcc ggcgcctgct tttcctccat
      181 gctcctgcaa tgcacgctgg ccactttata ctgctcgaag caggagatcg ccagcctgat
      241 cctgcatgaa tatgttattg ccccgaaaac ggacacgacc atttccgacg atatgcagga
      301 tcagttcaac aaagccgggc aggattatga taacagcaac tacggcgatg cgcgacgcca
      361 gtacgaaaag ctcgtacgga aagacagcac gtatttcaaa gccaccaacc gcgttggtct
      421 ctgctattac aaaacggaag attatgacaa tgcgatcatc tggttcaaaa aagccattgc
      481 gctgcagccg ctgatgatcg aaccgcgcgt gagcctggtg aggtgctaca aggaagagca
      541 caaatgggac gacgcgtata atgcctgcat tgacgggatc atcgtttacc cggacgaccg
      601 tctcttcatg ctcctcgatg aaacctgcga caagctcggc aaaacgttcg atcgccattt
      661 catgccacgg gattacatcc cgaacatggc caacaacgac gaccagggct ccatcgatga
      721 gagtccgtgg agcttttacc gcatggcgaa agacaagatc atggattact gcgacaagaa
      781 cggcgtcatc accaggacaa acgatgtaac caagcaaggc tacctcgaat cgttcagctg
      841 ggaatacatg ttgcagaagt cgacgccgga aggaaaggaa ttcccgttcg ccaaaaaaat
      901 gatgaacgaa ggttacatcg actgctattc catggtgagc ctgttccaca tcacgatgtg
      961 ggaccagtac cacgcgttct cgcaggcgaa tgcggatcgc atccggacct acatcacgac
     1021 ttatctcgtc aaataaatta aagagtgaaa acaaaaacgc cggacagaaa tatccggcgt
     1081 ttttttatgc gttcattttc cgcttcaatg cgaaattatc tttttcacca ctgttcccct
     1141 ggcggtgcgg aatgaaacga gatagacgcc acgtcccggc agcaccagtg ttccgtcgaa
     1201 ttcattcccc ggaatggcgg gcagcacttc ttttccgctg agatcataca cgcgcacttc
     1261 ctgcaggccg cttccggcgt aaccggaaaa atgcacagtt ccgtccgtca cggggttggg
     1321 atagcaggta atgccgggca acccgttctg cgagcttgtt cccgtgatgg tgttggtcat
     1381 gatctgctcg gcgaccagca tcggtgtgtg atcggcgttg tcgtacatcg tgtggaaaga
     1441 attgatcggg gctgtgttgt agctgaacat ctccgtcagc catttcgccg gcacgctcga
     1501 ataatacacg cgcgatgtaa cattcagcgc accagcatag ccgttcagga acacatgaaa
     1561 atggacgatg tcgccgcccg ttccttccac gggaccgttg taattgaaat caggatcatt
     1621 cgtcacaccg atgacgcgcg tggaatcgta cgaaggatgc gaagtggaaa atccgacggg
     1681 aacaatgcgg ttgtccttga ggcacgtatc cgcccgttcg agcacggttg tcggattgtt
     1741 gttcacgtcg cccatcacca tttcgtagat ctgcacgtcg ttcacgttat tgatcacgtc
     1801 gtggtgcggc tcgaagccgg gatcgcggcc gatgacattt cccgctgcgt catgaagtcc
     1861 gtccgcgaaa agcgtatcgc cgagattatc tgtcaccacg aactccaccc acgcgatgcg
     1921 tgccgggtaa cctgaaggaa atttatgccc gcatttgttc gtcagctgca catcataaaa
     1981 aaccgtatcc accgtgcgtg cggtctgcac cacctgcatg ttcagcgtgg aatcgcgcag
     2041 gtaacgcgtc gtgcggttga tcgtcgtgtc gaaattccac gactcgcatg tatttccaac
     2101 cgtggacata ttgccctgca tgagcttcag cataaatgca tttccgccga tgaggtgatg
     2161 gcgatcgaac gggaaccgtc cttcgatgaa cgggtaattc gtcgcgagct tgacggaatc
     2221 ctggatctgc gggatgtggc aatgctggca ggtgatgccg ttggccgggt aggacgaatt
     2281 tttccattcg tggtaagtgg cctgctcgat gtacgttcct cccgtgagat tacccgagag
     2341 atcggtggta ttggtgatga gcgtgtgaca gccggcgcac agttccgaat gcgtcatgtg
     2401 tgcgctgtac gaaggcgtaa agccaacgaa aaattccatc accgcgctta caggatcctg
     2461 caccgggccg aatacctgtt tctgcgcgta aaataaattt cccgtaaaat tatttccctg
     2521 cagcgtgtcc ttgatctcgt ggcagcccgt gcaattcacc ccgtcgagtc cgaaagaatc
     2581 cgtggcgagc atgttcatcg tgtaatattg ctgcccgagg taatgcgctt caaaatgccc
     2641 ggtcggcgca tggcatgtgg tgcatttgtc ttctgtttca agctgcagcc cgggattcac
     2701 cgcggtttcg tggctcactt ttgcgcgcca cagcggatct ttcgccgagt tcgccatcat
     2761 cgttccctgc cagtcgtcca tgatattcac atcttctccc caggaagtgt aaagcgaaat
     2821 atgtgtggga tcatgcccgt ggcacccgcc gcagcgtccg ccggtcgcga aatacgtgtt
     2881 ggtgtcatac ggctggctct ggaagaattc atggatgcgg gcgagatccc ttttgctgtg
     2941 gtagggccgg gcttcggcaa gctgcccgcc aagaccagta acgagcgcga tcagcaaggc
     3001 cgcaacaccc gccacaacca gtatcctgaa ctttgaaacg tactttttta tcacttcgtg
     3061 aggattttaa taagcattaa aaataaggac atccgggtag acctcaaaat gacctaaaca
     3121 tttgattttc ttattattat ttgttaatct cgtattctta aaccagaagc catgacaaaa
     3181 cacctaccca agtttattct tgcattggcc gcctccctgc ccgtcgcggc atttgcacaa
     3241 acgaccatca cccctttttc cgatcagaca tcccttctgc cctcgtcagt tttccatagt
     3301 ggaaatgcgg tgggcgtgtg cgatatgaac ggcgatttta aagatgatat cgtaagggac
     3361 agcatgaata agcagatgta tatcgagttc cagcaggcgc cgaacggaac gttcgcacaa
     3421 acagcttaca acaccggctc attcggttcc ccgtggggac tttgcat
//
LOCUS       DASTSF010000464        14606 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_964625,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000464 DASTSF010000000
VERSION     DASTSF010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 14606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14606
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_964625"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..542
                     /locus_tag="VFU15_13425"
     CDS             <1..542
                     /locus_tag="VFU15_13425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013684786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU4718836.1"
                     /translation="LSADLQVPSLETRIAILEKKLYIDGIELPREVIEYLAYSITTNI
                     RELEGAYISLLAQASLNKKAITLDLAKQMIDKFVKNTAREVSIDYIQKVVCDYFDLPI
                     ELLKSKTRKREVVQARQIAMYFAKNMTKSSLATIGMHCGGKDHATVLHACRTVNTRMD
                     TDKRFRAYIEELEKKLSIQ"
     gene            complement(600..1082)
                     /locus_tag="VFU15_13430"
     CDS             complement(600..1082)
                     /locus_tag="VFU15_13430"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HEU4718837.1"
                     /translation="MKRTRTTWLEKFNREQPPKIETAHGKFAEKFGEGKMVIASPKLV
                     DAIIRRIPKGKLATINMIRAELAKEFGVDYACPITTGIFAWIGAHAAEEMKKAGAKKI
                     TPWWRVVKEDGGLNPKYPGGEELHAKRLREEGFSIVQKSLRSKKKVVKNAAEYMPAHF
                     "
     gene            complement(1216..4167)
                     /locus_tag="VFU15_13435"
     CDS             complement(1216..4167)
                     /locus_tag="VFU15_13435"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HEU4718838.1"
                     /translation="MMIPVVKSGAQSFDFISYGVEEGLSQSEARCVMQDTRGYLWTGT
                     AGGGVCSYDGIKFTEYGKKDGLPGQVVNCLAEDSAGNIWIGTSTGVAFYDGKIFNHLI
                     DNRQLESGNISSLAVLKDRVLIGTGDGIFQYSGGKKMASLFAYARNVNGISVGDDGTV
                     WATSASTLWRISNGKCDSIPLPAAEMLERKLTCVTTDHHGLVYIGTDHGLLVYRVSTG
                     TFNGNTLSGTLGGDEVSALFAAADGTVWAGTMNNLVVKFAPDNSFVSYDRSNGLDAEA
                     VFGITVDDTKHLWLATKEQSLVYLRSESFSYYGNLKGMGSVSVFRIMEDHEGHIWTGS
                     KTDGLYEYDGKTTHEVLNGSRPFEQPFGIAEDKNHVIWVGHLNGVTGLVNGKAVKQLI
                     PGVHVRTLMCDSKGNLWIGTWGSGLFFYDGHEMKNYTVSGGQIPQDYVHSLLEDHLGN
                     IWIGTGNGLCRYDGKRFRTYGVEDGLCNTYIGSLAEDQYGNIWFHTDACIQRFDGRTF
                     RSYDEGNGLASSTYYFVACDAANQLWVGSNKGIDRVKIDEFGNFLSVKNYSRNEGFRG
                     IECNSRAVCVGSDKTLWFGTVKGVIRYFPSFDYPDNVEPRTHITGIRLFLEPTDWTWS
                     GIKESGWYHLPEKLVLNFDQNHLTFLYEGVHLGKSAEVSYKFMLAGFDSAWQPVTFSK
                     EITYANLPPGSYRFMVLAANPDGKWNSVPATSCNIVILPPPPPFYMNWWFIGIAALLV
                     TGILYYVIVGRTRRIMAQKEKLEAEVRERTNEISRQNEEKTLMLKEIHHRVKNNLQVI
                     SSLLNLQADGITDKHMLALFEDLRHRVNSMALIHEKMYQSRTLVNIDIQGYIRDLISS
                     LIDAYDSNKNIHTDFDVEEITFRIDTIVPLGLILNEIISNSLKYAFEGRSGGNLSVRL
                     HRTGAHSFRLEVGDDGVGMPADFDLEKTDSLGIQLIHMLCDQVNGKISMTTDKGTHYT
                     IDFSEDAKDRF"
     gene            4295..4774
                     /locus_tag="VFU15_13440"
     CDS             4295..4774
                     /locus_tag="VFU15_13440"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013619586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HEU4718839.1"
                     /translation="MTRILMVCLGNICRSPLAEGILRKRIDEAGLTNVEVDSAGTSGW
                     HEGEHPDHRSVHNARRHDVDISTLVSRKFSVADFERFDRIYVMDSSNYRDVTALAKNE
                     IHLAKVDFLLNCKWPGKNMAVPDPYYSGAEGFETVFHLVNEACLALVGSLQKENAGD"
     gene            4767..5483
                     /locus_tag="VFU15_13445"
     CDS             4767..5483
                     /locus_tag="VFU15_13445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEU4718840.1"
                     /translation="MTEKKGNLFLLPVTLDDATEPSSVLAGDALKAAHELKLFLAENE
                     KSARHFLKKAGTKWPLPEIKLFSMGKHGDESDIDLFIREIENGNDGGIISEAGCPAIA
                     DPGSVAVKKAHEKNIRVVPLTGPSSILLALMSSGFSGQRFRFHGYLPIDKNEKARALK
                     SIERAVAEKDETQIFIETPFRNNQLMDDLLRVLDASTMLCVACDLTSAREFVRTKRVS
                     EWKKQKPDLHKRPAVFLVGK"
     gene            complement(5491..6015)
                     /locus_tag="VFU15_13450"
     CDS             complement(5491..6015)
                     /locus_tag="VFU15_13450"
                     /inference="COORDINATES: protein motif:HMM:NF024724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEU4718841.1"
                     /translation="MKSFIEAILFSARSHSGQHRKDGHTPYINHPLEVMQLVSSATGD
                     GDILIAAVLHDVVEDTPVTGNEIAERFGPRIARIVIELTDDKFLPKEERKRQQLLTSG
                     QLSKEARLIRICDKICNVHDILYAPPRDWPVSRRKDYLEWANAVVNKIRGTHEELEKR
                     FDELYREGVKFLDK"
     gene            complement(6015..6311)
                     /locus_tag="VFU15_13455"
     CDS             complement(6015..6311)
                     /locus_tag="VFU15_13455"
                     /inference="COORDINATES: protein motif:HMM:NF017103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HEU4718842.1"
                     /translation="MMKKKGNEYSLKEAIQEMMQSYRLEGKMNEFRLVSAWEKVMGPV
                     IAKYTRGIKIENRVLYVELNSAALREELLFGKQKMIRMLNEEAGAAVIDDVILR"
     gene            6529..8472
                     /locus_tag="VFU15_13460"
     CDS             6529..8472
                     /locus_tag="VFU15_13460"
                     /inference="COORDINATES: protein motif:HMM:NF024974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HEU4718843.1"
                     /translation="MWSQTADDVTEDSLAGFDLGVALAQTGEADEAYEANNQIRIAQR
                     DYIDQKYHLGKYAPDYQYPGKVYNNAVFPVSSCTNVDFETGDFTGWSGQIGDNTGGCY
                     SPFTSPQNGIFSSGVDAPVSNSAARHTIITPAFGNDPYGGFPGVPSGSGGFTARMGGQ
                     TPNYQGEKLEQTFTVSPTSTSFAYRYAVVFNDPPFGHPPTAKPYFRIEVLDQNGQPIS
                     NCTQFFDTSHVNSPGFFLSPQAAPNGDPVYYKPWTTVNFDLTGFVGQNVTIRFTVAGC
                     TQSGHFGYAYIDCSCSALAASINFCPGNTFLYLGAPDGYAAYQWLDPQHNPIPGATND
                     TLLVNNPVVGDTFFVYLTSAADTSCHNTLPVVLEYTHIFPNATATNASCFGYKDGTLS
                     ASGTLGFPPYTYNWNTLPPQSGQSISNVGAGTYIVHMTDSLGCDAYDTTVITQPARID
                     TSLFTYAFCYGDPHITISAPPGFVQYTWIGPNGDTLSANPPNSVYVNGPQIGDEYTCI
                     LYPSTGCPVYDTVALNFNPPSIFFSADSTVNVFTPNGDGKNDYFYPYYDYTVATQASS
                     PGGGQPAYDFFDLYIATYEIKVYNRWGQQVFYSNDYNYGWDGMIDKDKASEGVYYWIA
                     TYTTRCSQTPTPIVKTGFVHLIR"
     gene            8576..9259
                     /locus_tag="VFU15_13465"
     CDS             8576..9259
                     /locus_tag="VFU15_13465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014680850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEU4718844.1"
                     /translation="MATLSEENYLKAIWKLAPDPDAGVSTGEIAAEVKTSAASVTDML
                     KKLAARKLITYQPYHGVSLTVQGQRLAADVVRRHRLWEVFLHEKLGFGWEEVHDIAEQ
                     LEHIHSEALTDRLDRFLGFPKTDPHGDRIPDKTGKISGPSAVPLSVFNDGEYGIISGV
                     INHAPSFLLFLEKHGLKPGAAIEMKERMEFDDSMNIRVNGKKTMTLSRDASSSILVIR
                     KGKKQNRKS"
     gene            9256..11136
                     /locus_tag="VFU15_13470"
     CDS             9256..11136
                     /locus_tag="VFU15_13470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014680849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HEU4718845.1"
                     /translation="MSNHSLREVYASVDTTNKKTGWKKIIAFFGPAYLVSVGYMDPGN
                     WATDIAGGSRFGYALLWVLVLSNLMALLLQSLSARLGIVSGLDLAQASRGEYPKRMNF
                     FLWVLAEIAIAACDLAEVIGMAIGLQLLFGLPLMWGVLITVFDTLLLLMLQRFGIRKM
                     EALIIGLISVIGLAFIIELFYSKPDAGEIFAGLKPSLPGGAALYIAIGIIGATVMPHN
                     LYLHSSLVQTRKIRRTREAIRQAIRLNVFDSAIALNLALFVNAAILILAGATFFSRGI
                     YNVESIHSAHQLLSGLLGSKLAPALFAIALIAAGQSSTLTGTLSGQIVMEGYLNIRLQ
                     PWMRRLITRLLAIIPAIITLLIAGEKGTEDLLILSQVILSLQLGFAVIPLIHFVRSKK
                     LMGEFRAPLSAIIPAWICAALIVVLNGKLVMEFTQDLLHKSAHPVIIWLTLVPVILLC
                     AFILLWITVRPFIRKKHETILSDPHEAPVSFEFTPPSRYKKIAVCVDFSSSDSIAISQ
                     ATRNGGQEAVYFLIHITETAGARVMNEETGDMETQYDEQMLNEYTDRLQKEGYHVETQ
                     LGYGNPKERIPEVVNGSGSDLLVMGAHGHNLIKDLLFGTTVNAVRHRVDIPVLVVKKE
                     KK"
     gene            11196..12311
                     /locus_tag="VFU15_13475"
     CDS             11196..12311
                     /locus_tag="VFU15_13475"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEU4718846.1"
                     /translation="MDQEHYSAPQEEAPPKRPFYDGLIPRGDYFFTPIIININILVFL
                     AMVISGVSFTFPTASDLLRWGTNYKPLTMGGQPWRMFTSMFLHFGIIHLAVNMYSLFS
                     LGRIIEPFVGRWRFLVLYLCAGLGGSAVSLWWHTNAAGAGASGAIFGLAGIFGALLTT
                     DLIRKEYRGKMLRSIAQAIVINLAIGLMGSIDNSAHIGGLLTGAIGGYLIYFDLRSLY
                     RNGQKKYTGLLGSVAVTVVIIAGLWHFTSVPPDFDAMVKRFSAEDQKASLYYLQMDNA
                     MESDIRKNYILPYQHCVAITDSMEDQDLNDFGTKYVALLRLYAETRLNTGEALLRERQ
                     AGTTGVPHTAAVLKLRGDSLLQVINDLEKKNAEDGDK"
     gene            complement(12333..>14606)
                     /locus_tag="VFU15_13480"
     CDS             complement(12333..>14606)
                     /locus_tag="VFU15_13480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718847.1"
                     /translation="GGRKRNGTPRKDPHQRLMTALLRNKILFQGVLLLCSCISLFAQN
                     EETRRKIDMDRRSLSFLDDKSLEKSREYIRMDSTFYIGYMYQGAYMYYRAADEYGFNQ
                     AIVPLEKAMTLLEKDYDKELRTRTSDLFVYYRVTTYQYDYCYIAYWLQECYQDVEKPD
                     KAFEVLQRVRDRNLQFENGIETFNTMAWIYHRNRMYTSKNFRFLKNSVAANDSMAHLY
                     LDSAIYKTENDAALNVGLYDASTVNAQLYYTYHYKAILFDYEFNIDSSDYYYNILLRV
                     GYYSSNNYANYKYLLGDFATADQFYREAEHREGSVEKRTREYFYMRGLIDIYKGEPDS
                     SASLLKDVIDEQGYTPGFGWHSIAYARALQYEGLTAESQKAVNKAAQFHELHIGTTWG
                     QEQYNLCIAMLNYENARHFEKEYYFENSAWWSWMNPAAWLRYSRYRFEQHNEKMILAQ
                     LAADDPERQQVIYPLFSSENLISFDEVNELVEGFGNQYFIKIYTRMLANDKRPLVLKY
                     LRYTLGKLYLEDGQSDKAMEFFQMVINDPGADDPYGKLLNARACEGMALASGNAGDKD
                     YWTGKMYETYPQLLPYSKLTMKFKTDDDVLLHGARSGAMMIAILLFIFGILSTVALYL
                     LRGFGKIRAAKLVVSLPLIVFSALAGSVTVWNASGKGLDTATRIKNDMHNCGIEFTDK
                     NDYPRLDLQITSAGDTTQVDYTVTGNLVSQTRSGSLSFSKDELDDAGKLIVYRLFGIE
                     KKKIGETVPPEKTDKKK"
BASE COUNT         3319 a         4054 c         3766 g         3467 t
ORIGIN      
        1 gactttccgc cgacctccag gttccgagcc tcgaaacgcg catcgccatc ctcgagaaaa
       61 aactttacat cgacggcatc gagcttccgc gcgaagtgat cgaatacctt gcgtacagca
      121 tcaccaccaa tatccgcgag ctcgaaggcg catacatttc actgcttgcc caggcgtcgc
      181 tgaacaagaa agccatcacg ctcgatcttg ccaagcagat gatcgacaag ttcgtgaaga
      241 acaccgcaag ggaagtttcc atcgactaca tccagaaagt ggtatgcgat tatttcgatc
      301 ttcccatcga gctgctgaag tcgaaaacac gcaagcgcga agttgtgcag gcccgccaga
      361 tcgcgatgta ctttgcgaag aacatgacca agtcttcgct cgccacgatc ggaatgcatt
      421 gcggaggaaa ggatcacgca accgtgcttc acgcgtgccg caccgtgaac actcgtatgg
      481 ataccgacaa acgtttccgc gcttatatcg aagagctcga aaagaaactc agcatccagt
      541 aaaaattccc ccttcaggaa cgcccggtcg caaatgctgc cgggcgtttt tgtttccggt
      601 cagaaatgcg cgggcatata ctccgctgcg ttcttcacca ctttcttttt gctgcggaga
      661 cttttctgca cgatcgaaaa accttcttca cgcaatcgct tcgcgtgcag ctcttcccca
      721 cccgggtact tcgggttcag tccgccgtcc tcttttacca cgcgccacca tggcgtgatc
      781 ttcttcgcgc cggccttttt catttcttca gccgcatgcg cgccgatcca cgcgaaaatc
      841 ccggttgtaa tggggcacgc ataatcgaca ccgaactctt tcgccagctc ggcgcggatc
      901 atattgatgg tggcaagttt tcctttggga atgcggcgga tgatcgcatc gaccagcttc
      961 gggctggcga tcaccatttt tccttcaccg aatttttccg cgaatttccc gtgcgcggtt
     1021 tcgatcttcg gcggctgttc cctgttgaac ttttccagcc acgttgtcct ggtcctcttc
     1081 atacctgtcg tgattatttt tccaaacctg caaataccgg gataaaaaac aaacacttcg
     1141 aatcatgaat agccgtgagg ttcgtcaaat tccatacggc aggcggacag ctcaggcgga
     1201 acctatttat aaggatcaga acctgtcttt cgcgtcttct gaaaaatcga tcgtgtaatg
     1261 tgtgcccttg tcggtcgtca tcgaaatttt tccgttgacc tggtcgcaca gcatgtggat
     1321 gagctgtatg ccgagcgagt ctgttttttc caggtcgaaa tcggccggca ttcccacgcc
     1381 gtcatcaccc acttcgagcc ggaacgaatg cgctcctgtc cggtgcaggc gcacgctgag
     1441 gtttcccccg ctccttcctt cgaaggcata cttgagcgaa ttcgaaataa tttcattgag
     1501 gataagcccg agcggaacga tcgtgtcgat gcggaaagtg atctcttcca cgtcgaaatc
     1561 cgtgtgaata tttttgttcg aatcgtatgc gtcgatgagc gagctgatga ggtcgcggat
     1621 atagccctgg atatcgatgt tcaccagcgt gcggctctgg tacatttttt cgtggatgag
     1681 cgccatggaa ttcacgcggt gacggagatc ttcgaacagc gcgagcatgt gcttgtcggt
     1741 tattccgtcg gcctggagat tcagcagcga agaaataacc tgcaggttgt tcttcacgcg
     1801 gtgatgaatt tctttcagca tgagcgtttt ctcttcgttc tgccgcgaaa tttcattcgt
     1861 gcgttccctg acctcggctt cgagcttttc tttctgcgcc atgatgcgcc gcgtccgccc
     1921 gacgatgacg taataaagta tcccggtgac cagcaatgcc gcaataccga tgaaccacca
     1981 gttcatataa aatggcggcg gtggcggaag gatgacgata ttgcaggaag tggcgggaac
     2041 ggaattccat tttccgtcgg ggtttgcagc aagaaccatg aaccggtagc tgcccggcgg
     2101 caggttggca tacgtgatct ctttcgaaaa tgtcacgggt tgccaggcgc tgtcgaaacc
     2161 ggcaagcatg aacttgtacg acacttccgc cgatttcccg agatgaacgc cttcatagag
     2221 gaaggtcagg tggttctggt cgaaattcag caccagcttt tccgggagat gataccagcc
     2281 ggattctttt atgcccgacc aggtccagtc tgtaggttcg agaaaaaggc ggatgcccgt
     2341 gatgtgcgtg cgcggctcca cgttgtccgg gtaatcgaag gaagggaaat aacggatgac
     2401 gcctttgacg gtaccgaacc agagcgtttt atcgctgccc acgcaaacag cacgcgagtt
     2461 gcattcgatc ccgcggaatc cttcgttgcg cgaataattt ttcacggaaa gaaaatttcc
     2521 gaactcatcg atcttcacgc gatcgatgcc tttgttcgaa ccaacccaga gctggttggc
     2581 tgcatcacag gcgacaaaat aatacgtgct cgacgccagc ccgttccctt catcatacga
     2641 acggaacgta cgcccgtcga aacgctgtat gcacgcgtcg gtgtgaaacc agatattgcc
     2701 gtactggtct tccgccagcg acccgatata cgtattgcag aggccgtcct cgacgccgta
     2761 cgtccggaac ctttttccgt cgtaacggca cagcccgttt cctgtcccga tccagatgtt
     2821 gccgagatga tcttccagca gcgaatgaac atagtcctgc gggatctgcc cgccgctgac
     2881 cgtgtaattt ttcatctcgt gcccgtcgta gaaaaaaagt ccgctgcccc acgttcctat
     2941 ccagagattt cctttcgaat cgcacatcag cgtacgcaca tgcacgccgg gaatgagctg
     3001 cttcactgct ttcccgttga cgaggcccgt aacgccgttc agatgcccga cccagatgac
     3061 atgattttta tcttccgcga tcccgaacgg ctgctcgaac ggtcttgatc cgttgagaac
     3121 ctcgtgcgtc gttttcccgt cgtattcata aagtccgtcg gtcttcgatc ccgtccagat
     3181 gtgcccttca tgatcttcca tgatgcggaa cacgctgacg gaacccattc ctttcaggtt
     3241 gccgtaatag ctgaacgact cgctgcggag ataaacaagg ctctgctctt tcgtggcgag
     3301 ccacaggtgt tttgtgtcgt ctaccgtaat gccgaagacc gcctcggcat cgagaccgtt
     3361 ggaacgatca taggaaacga atgaattgtc gggagcgaat tttacaacga ggttattcat
     3421 cgttccggcc cacacggttc cgtccgcagc tgcgaacaag gccgaaactt catcaccgcc
     3481 gagcgttccg ctcagtgtat ttccgttgaa cgtcccggtg gacacgcggt aaacaagcag
     3541 tccgtgatcc gttcctatat agacaagccc gtgatgatcg gtggtcacgc aggtgagttt
     3601 ccgctccagc atttccgcgg ccggaagcgg gatgctgtcg cacttcccgt tgctgatccg
     3661 ccaaagcgtt gaagcggatg tcgcccacac ggttccgtca tcaccgaccg aaattccgtt
     3721 cacgttccgt gcgtaagcga acaggctcgc catttttttt ccacctgaat actggaagat
     3781 gccgtcaccc gttccgatga gcacgcggtc tttcagaaca gcgagggagc tgatgttccc
     3841 ggattcgagc tgcctgttat cgatgaggtg gttgaatatc tttccatcgt aaaacgcgac
     3901 gcctgtgctt gtcccgatcc agatattgcc ggcggaatct tcagcgaggc aattcaccac
     3961 ctgcccgggc agcccgtcct tttttccgta ttcggtgaat tttatcccgt catagctgca
     4021 cacgcctccg cctgcagtgc cggtccacag gtaaccacgg gtatcctgca tgacgcagcg
     4081 cgcttcggac tgggaaagac cttcttcaac accgtaggaa ataaaatcga aagattgcgc
     4141 tcctgacttc actaccggga tcatcatcac cgtgatgaaa aaaaaacgga aggagctatt
     4201 tcggataagg tctttcagga ttggaaaaat tagggtctga tcaaatttat caaaaagaac
     4261 ttatgaaggg attaactttg gccaaaagaa aagtatgacg cgcatactga tggtttgtct
     4321 cgggaacatc tgccgttcgc cactggcaga aggtattctc cgcaaacgca ttgacgaagc
     4381 cgggttgacc aatgtggaag tggattctgc gggaacttca ggatggcacg aaggcgagca
     4441 ccccgatcac aggtcggtgc acaatgcacg ccggcacgac gtggatattt ccacgcttgt
     4501 gtcgcggaaa ttttccgttg ccgatttcga gcggttcgac cgcatctatg tcatggacag
     4561 ttcgaattac cgcgacgtga cagccctggc aaaaaacgag atccatcttg cgaaagtgga
     4621 tttcctgctg aactgcaaat ggccgggaaa gaacatggct gttcccgatc cgtattacag
     4681 tggtgcggaa ggatttgaaa cggtgttcca cctggtgaac gaagcctgcc ttgcgctcgt
     4741 gggttcgctg caaaaagaaa atgcaggtga ctgaaaaaaa aggaaacctt ttcctcctcc
     4801 cggtgacgct ggatgacgca acggagccgt cttccgtgct tgcaggagac gcgctgaaag
     4861 cagcgcatga gctgaagctc tttctcgctg aaaacgaaaa gtcggcaagg catttcctga
     4921 aaaaagcggg aacaaaatgg ccgctccccg agataaaact gttttcgatg ggaaagcacg
     4981 gcgatgaaag cgatatcgat ttattcatcc gggagatcga aaacgggaac gatggcggca
     5041 ttatttccga agccggttgt cccgccattg ccgatccggg atccgttgcc gtaaaaaaag
     5101 cgcatgaaaa aaacatacgc gttgttccgc tgacgggccc ttcctctatc ctgctcgcgc
     5161 tgatgtcgtc gggattcagc gggcagcggt tccgctttca cggctatctt cccatcgata
     5221 aaaatgaaaa ggcgcgcgcg ctgaaatcga tcgaacgcgc agtggcggaa aaagacgaaa
     5281 cccagatctt cattgaaacg cctttccgca acaaccagct catggatgat ctcctgcgcg
     5341 tgctcgatgc atcgacgatg ctttgcgtgg cgtgcgacct gacctcagcc cgggaatttg
     5401 tgcggacaaa acgcgtgagc gaatggaaaa agcagaagcc tgatcttcac aagcggcccg
     5461 ctgttttcct agtcgggaaa taaacgtgcg ttatttatcg aggaatttca cgccttcgcg
     5521 gtagagctcg tcaaaacgtt tttccagctc ttcgtgcgtg ccgcgaatct tattcaccac
     5581 ggcgttggcc cattcgaggt aatctttcct tcgtgatacc ggccagtcgc gcggcggcgc
     5641 gtagaggata tcgtgtacgt tgcagatctt atcacagatg cggataagac gcgcttcctt
     5701 cgaaagctgg ccggaggtca gcagctgctg tctcttacgc tcttccttcg gaaggaattt
     5761 atcatccgtc agctcgatca cgatgcgcgc aatgcgcgga ccgaaacgtt cggcgatctc
     5821 attgccggtg accggcgtgt cttccaccac atcgtgaagc accgcggcga tgaggatatc
     5881 gccgtcaccg gtagctgagg aaaccagctg catgacttcg agcgggtggt tgatgtaggg
     5941 cgtatgtccg tctttgcggt gttgcccgga atggctgcgg gcggaaaaaa gaatggcttc
     6001 aataaacgat ttcatcaccg caaaattaca tcatcgatga ctgcggcccc ggcttcttcg
     6061 ttcagcatcc ggatcatttt ttgttttccg aataacagct cttcacgcag cgcggcggaa
     6121 ttcagctcga catacagcac gcggttctcg atctttatgc cccgggtgta cttggcgatc
     6181 acaggtccca tcactttttc ccatgcgctc accagcctga attcgttcat cttcccttcg
     6241 agccggtaag actgcatcat ttcctgtatc gcctccttca gtgaatattc atttcccttt
     6301 ttcttcatca taacttcctg aaattaggaa aaacatcggt gactgcccgg aaaagtccgc
     6361 ttaaatggct gtatcctttt tcatttcacc gggttataaa agtggcaaac acaaataatt
     6421 ttgaaaatca ggctactatg agtatattag ccggtctcgc aaacacattt tttcatgaac
     6481 aaaaaagcta ccctgatcct tgcggctcta ctctgttttg gtttccgcat gtggtcccag
     6541 actgcagatg acgtaaccga agattcgctg gccggtttcg accttggcgt tgcgctcgct
     6601 caaacgggcg aggcggatga agcgtacgaa gccaataacc agatacgcat tgcacagcgc
     6661 gattacattg accagaagta ccacctcggg aaatatgcac cggactacca gtacccgggc
     6721 aaagtataca acaacgctgt tttcccggta agctcctgca cgaacgtcga tttcgaaacc
     6781 ggtgatttca ccggctggtc aggacagatc ggcgataaca ccggcggctg ttacagtccc
     6841 tttacttctc cgcagaacgg gatcttcagc tcaggcgtcg atgcacctgt ttcgaattcc
     6901 gcagcacggc acacgatcat cacgcccgct ttcggtaacg atccgtacgg cggctttccc
     6961 ggtgtaccca gcggttccgg tggttttacg gcgcgcatgg gcggacagac accgaactac
     7021 cagggcgaaa aacttgaaca gacgttcacc gtttccccga catctaccag ctttgcttat
     7081 cgctatgcgg tcgtgttcaa cgatcccccg ttcggtcatc ccccgacagc gaaaccgtat
     7141 ttccgcattg aagtgctcga ccagaacggc cagccgatct cgaattgtac acagttcttc
     7201 gatacgtccc acgtcaacag tcccggcttt tttctttcgc cgcaggctgc ccccaacggg
     7261 gacccggttt attacaaacc ctggacgacc gtgaacttcg accttaccgg tttcgtggga
     7321 caaaacgtga ccatacgttt caccgttgca ggatgtaccc agtcgggaca cttcggctac
     7381 gcctatatcg attgctcgtg ctccgctctt gcagcttcca tcaatttctg cccgggcaat
     7441 acgttcctct atctcggtgc gccggacggc tatgctgcat accaatggct ggacccgcag
     7501 cacaacccga taccgggggc cacgaacgac acgctgcttg tgaataaccc cgtggtcggg
     7561 gataccttct tcgtgtacct gacttcagct gccgatacga gctgtcacaa cacccttccg
     7621 gttgtccttg aatacacgca cattttcccc aatgcaaccg cgaccaatgc gagctgcttc
     7681 ggctacaagg atggaacgct ttccgccagc ggaacgctgg gattcccgcc ttatacatat
     7741 aactggaata cacttcctcc gcagagtggc cagagtataa gcaacgtggg tgcgggaacg
     7801 tacattgtcc acatgaccga ttcgctggga tgcgacgcgt acgacacaac tgtcattacg
     7861 caaccggcaa ggatcgatac gtctttgttt acttatgcat tctgctatgg cgatccgcac
     7921 atcacgatca gcgctccgcc gggttttgtg cagtacacgt ggatcgggcc gaacggtgat
     7981 acgctgtcgg ccaatccgcc gaacagtgtt tatgtcaacg gtccgcagat cggcgatgaa
     8041 tacacgtgca tactttatcc gtcgacggga tgcccggtgt acgataccgt agcgctcaat
     8101 ttcaatccgc cgagcatttt cttctcggcc gattccacgg ttaacgtctt caccccgaac
     8161 ggggacggaa agaatgacta cttctacccg tattacgatt acacggtggc cacccaggct
     8221 tcgtcacccg gcggcggaca gcccgcgtat gatttctttg atctttacat tgcaacgtac
     8281 gagatcaagg tatataaccg ctggggccag caggtattct actccaacga ttataattac
     8341 ggatgggacg gcatgatcga taaagacaaa gccagcgaag gtgtttatta ctggatcgcg
     8401 acatacacca cgcgttgttc gcaaacgcct accccgatcg taaaaacagg gttcgttcac
     8461 ctcatccggt aacgccaccg ggccagacct acaaatcccc cgtacgatgc gggggatttt
     8521 tttgttcttt tttattttta gtctcatcta aatatttatt tttgtacgat taaaaatggc
     8581 aacactctcc gaagaaaact acctgaaggc gatctggaag ctggcgccgg atcccgatgc
     8641 gggcgtaagc acgggcgaga tcgcagcgga agtgaaaaca agcgcggcgt cggtgacgga
     8701 catgctgaaa aagctcgcgg cgcggaagct gatcacgtac cagccgtatc acggcgtctc
     8761 gctgacagtc cagggacagc gtctcgccgc ggacgttgtg cgcaggcacc ggctgtggga
     8821 agttttcctt catgaaaagc tcggtttcgg atgggaagag gtgcacgaca ttgccgaaca
     8881 gctcgagcac atccattcgg aagcgcttac cgacaggctc gaccgtttcc tcggcttccc
     8941 gaaaaccgat ccgcacggcg accgcattcc cgacaagacc ggaaaaattt ccgggccgag
     9001 cgcagtgccg ctttccgttt tcaatgacgg ggaatacggg atcatttccg gcgtgatcaa
     9061 ccatgcgccc tcgttcctgc tgttcctgga aaagcacgga ttaaagcccg gggccgcgat
     9121 tgaaatgaaa gagcgcatgg agtttgacga ctcgatgaac atacgcgtga acggaaagaa
     9181 aacaatgacg ctgagccgtg acgcttcgtc gtcgatcctt gttatccgga aaggaaaaaa
     9241 acaaaacagg aaatcatgag caaccattcg ctcagggaag tgtatgcttc cgttgacaca
     9301 acgaataaaa agaccggctg gaaaaagatc atcgcttttt tcggaccggc gtacctcgtg
     9361 agtgtgggct acatggatcc cggcaactgg gcgacggaca tcgcgggcgg cagcaggttc
     9421 ggctatgcgc tgctgtgggt gctggtgctt tccaacctca tggcgttgct gttgcagagc
     9481 ctcagcgcac ggctgggcat tgtttcgggt ctcgacctgg cgcaggcgtc gcggggcgaa
     9541 tatccgaagc ggatgaactt tttcctgtgg gttctcgccg agatcgcgat cgccgcctgc
     9601 gacctggcgg aagtgatcgg catggcgatc ggcttacagc tgctgttcgg tctcccgctc
     9661 atgtggggcg ttctcatcac tgttttcgac acgctgctgc tgcttatgct ccagcgtttc
     9721 ggcatacgga aaatggaagc gctgatcatc gggctgatca gcgtgatcgg tcttgcgttc
     9781 atcatcgaat tattttacag caagcccgat gccggagaaa ttttcgccgg gctgaaacct
     9841 tcgcttcccg gcggagcggc gctgtatatt gccatcggta tcatcggcgc aaccgtgatg
     9901 ccgcataatc tctacctgca ctcttcgctg gtgcagacac ggaagatccg gcgcacgcgt
     9961 gaggcgatac gccaggcgat ccggctcaat gttttcgatt cggccatcgc gctcaacctt
    10021 gcgttgttcg tcaacgcagc cattctcatc cttgcgggcg ccacattttt cagtcgcggc
    10081 atctacaacg tcgaatccat ccattccgcg caccagctgc tttcgggatt gctcggatcg
    10141 aagctggccc ctgccctttt cgcgatcgcg ctgatcgccg cggggcaaag ctcgacgctt
    10201 accggaacgc tgagcggaca gatcgtgatg gaaggatatc tcaacatccg cctccagccg
    10261 tggatgcgcc gtttgatcac gcggctgctt gcgatcattc ccgccataat cacattgctc
    10321 attgccgggg agaaaggaac ggaagacctt cttatcctca gccaggtcat cctcagcctg
    10381 cagctcggct tcgccgttat tccgctaatc catttcgtgc gcagcaaaaa gctgatgggg
    10441 gaattccgcg cacccctttc agcgatcatt cccgcctgga tctgcgcagc gctcatcgta
    10501 gttctcaatg gaaagctggt gatggaattc acgcaggacc tgctgcacaa aagcgctcac
    10561 ccggttatca tctggctcac gctcgtcccg gtcatcctgc tgtgcgcatt cattctcctc
    10621 tggatcaccg tgcggccttt catcaggaaa aagcacgaaa cgatcctgtc ggatccgcat
    10681 gaagcgccgg tcagcttcga attcacgcca ccttcccgtt acaaaaagat cgcggtgtgc
    10741 gtcgattttt cgtcaagcga cagcatcgcc atcagccagg ctacgcggaa cggcggacag
    10801 gaagccgttt atttccttat tcacataaca gaaacggcag gtgcacgcgt gatgaatgaa
    10861 gaaacgggcg atatggaaac acaatacgac gaacagatgc tgaacgaata cacggaccgc
    10921 ctgcagaaag aaggatacca cgtggaaacg cagctcggtt acggcaaccc caaggagcgt
    10981 atcccggaag tcgtgaacgg atccggcagc gaccttctcg tgatgggagc acacggtcat
    11041 aatcttatca aagatctttt gttcggcaca acggtgaatg ccgtacggca tagagtggac
    11101 attcccgtgc tcgtcgttaa aaaagaaaaa aaataattct gcacatttcc ggcacctgca
    11161 tcacgcattt tctttccttc tacctttgat gcccgatgga ccaggaacac tattcagcgc
    11221 cacaggaaga ggcgccgccg aagcgaccgt tttacgacgg gctgatcccg agaggcgatt
    11281 atttcttcac gccgatcatc atcaacatca acatcctcgt ttttctcgcg atggtgatct
    11341 cgggcgtaag cttcacgttc ccgaccgcca gcgacctgct tcgctggggc accaattaca
    11401 agccgctcac gatgggcggg cagccgtggc gcatgttcac gtcgatgttc cttcatttcg
    11461 ggatcattca cctggcggtg aacatgtatt cgctgttcag tctcggaaga atcatcgagc
    11521 cgttcgtggg acgctggcgt tttctcgtgc tgtacctgtg cgcggggctg ggcggaagcg
    11581 cggtgagctt gtggtggcac acgaacgcgg cgggcgcggg cgcatccggc gcgatcttcg
    11641 gactcgcagg aattttcggt gcgctgctga caaccgatct catccggaag gaataccgcg
    11701 gaaaaatgct gcgcagcatc gcccaggcca tcgtcatcaa tcttgcgatc ggcctgatgg
    11761 gatcgatcga caattccgcg cacatcggcg gattgctcac gggcgctatc ggcggttatc
    11821 tcatttactt cgacctgcgt tcgctttacc gtaacgggca gaaaaaatat acaggcctgc
    11881 tcggcagcgt tgccgtcacg gtcgttatca ttgccgggct gtggcatttc acttccgtcc
    11941 cacccgattt cgacgcgatg gtcaaacgtt tcagcgcaga agatcaaaaa gcttcccttt
    12001 attatttgca aatggacaat gcaatggaga gcgacatccg gaaaaactat atccttcctt
    12061 accagcattg cgtggcgatc accgattcga tggaagacca agacctgaat gatttcggaa
    12121 cgaaatacgt agcgctgctc aggctttatg cagaaacaag gctgaatacg ggggaagcgc
    12181 ttctccggga acgccaggca ggaacgaccg gcgtcccgca caccgctgcg gtgctcaagc
    12241 ttcgcggaga ttcgctgctg caggtcatca acgacctgga aaagaaaaac gcggaagacg
    12301 gcgacaaata accgggctgc gcgaaccgaa aattatttct tcttatccgt tttttccggg
    12361 gggaccgttt caccgatctt cttcttttca attccgaaca aacggtaaac gatcagttta
    12421 ccggcgtcat ccagctcatc tttggaaaaa ctgagcgagc ctgatctcgt ctggctcacg
    12481 agatttcctg ttacggtata atcgacctgc gttgtatcgc cggctgacgt gatctgcagg
    12541 tcgagccgcg ggtaatcgtt cttgtccgtg aattcgatcc cgcagttgtg catgtcattc
    12601 tttatccgcg tcgccgtgtc gagcccttta cctgaagcat tccacaccgt aacagagccg
    12661 gccagcgctg agaaaacgat cagcggcagg gagacgacca gcttcgccgc ccggatcttt
    12721 ccgaaaccgc gcaaaagata aagcgcaact gtcgagagga tcccgaatat gaaaagcagg
    12781 atggcgatca tcatcgcccc gctgcgcgca ccgtgcagca ggacatcatc atccgttttg
    12841 aatttcatcg tgagcttcga ataaggaagc agctgcgggt aagtctcgta cattttcccg
    12901 gtccagtaat ctttgtcgcc cgcattaccg gaggcgagcg ccattccctc gcatgcgcgc
    12961 gcattcagca gctttccgta cggatcgtcg gcaccgggat cgttgatcac catctggaaa
    13021 aattccatcg ctttgtcgct ctgcccgtct tcgaggtata attttcccaa tgtatagcgc
    13081 aggtatttca gcacgagcgg gcgcttatcg tttgccagca tgcgcgtgta gatctttatg
    13141 aaatactggt tcccgaatcc ttccacgagc tcattcactt catcgaagct gatgagattt
    13201 tccgaggaaa aaagaggata gataacctgc tgccgttccg gatcatccgc agcgagctgc
    13261 gcaaggatca tcttttcatt atgctgttcg aaacggtaac gcgaataacg cagccacgcg
    13321 gcaggattca tccacgacca ccacgcgctg ttctcgaaat aatattcctt ttcaaaatga
    13381 cgcgcattct cgtaattgag catggcgatg cagagattgt attgttcctg tccccatgtc
    13441 gtaccgatat gcaattcatg gaactgcgcc gctttgttca ccgctttctg gctttcggcc
    13501 gtcagtcctt cgtactgcag cgcccgcgcg tacgcaatgg aatgccagcc gaaaccgggc
    13561 gtgtagccct gctcgtctat cacgtctttc agcagcgagg cagacgaatc cggttctcct
    13621 ttgtaaatat cgatgagccc gcgcatgtaa aaatattccc gcgtccgttt ttcgaccgaa
    13681 ccttcgcggt gctccgcctc gcggtaaaac tggtcggctg tcgcaaaatc cccgaggaga
    13741 tatttatagt tcgcgtaatt gttgctgctg taataaccta cgcggagcag gatgttgtaa
    13801 taataatccg acgaatcgat attgaattca taatcgaaaa gaatggcctt gtagtgatag
    13861 gtgtaataca gctgcgcatt gaccgtggac gcgtcgtaga gcccgacgtt gagcgcagcg
    13921 tcgttctctg ttttataaat ggccgaatcg agatacaggt gcgccatcga atcgttggct
    13981 gcgaccgaat ttttcaggaa ccggaaattc ttcgacgtat acatccggtt gcggtgatag
    14041 atccacgcca tcgtgttgaa ggtttcgatg ccgttctcga actgcaggtt cctgtcgcgc
    14101 acacgttgca gcacttcaaa tgctttgtcg ggtttttcca catcctggta acattcctgc
    14161 agccagtaag cgatgtagca gtaatcgtac tggtacgtgg tgacacggta gtaaacgaaa
    14221 aggtcgctgg tgcgtgtgcg gagctctttg tcgtaatctt tttcaagcag cgtcatcgct
    14281 ttttccagcg gaacgatcgc ctggttgaac ccgtattcgt cggccgcacg gtaatacatg
    14341 tacgcgccct ggtacatata gccgatgtaa aatgtgctgt ccatacggat atattcccgt
    14401 gatttctcca ggcttttgtc atcgagaaag gaaagcgagc gacggtccat gtcgatcttc
    14461 ctcctcgttt cttcgttctg cgcaaaaagg gaaatgcagg agcacagcaa cagcacacct
    14521 tgaaaaagaa tcttatttct gagcagcgcg gtcatcagcc gttgatgcgg atccttacga
    14581 ggcgttccat ttctttttcg accacc
//
LOCUS       DASTSF010000465         4763 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1368968,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000465 DASTSF010000000
VERSION     DASTSF010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4763
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1368968"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..599
                     /locus_tag="VFU15_13485"
     CDS             <1..599
                     /locus_tag="VFU15_13485"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEU4718848.1"
                     /translation="DNSSISAAIQPDVIFEPGVQKYAVSYYDSTASALLLFSTGYNVN
                     ASSSWTIVSAQYNDATNNLKAAHPQIAWNPNTTSAVCAWNAEGVSTNGVSMFDAEVVS
                     TNVDQLIAAADFVSPYPNPSSGITTFEFNLKEQRDVQLHVTNMMGQEVMAPVDMGSLL
                     PGNYTLPVDFSALSNGTYIYTMQVGNEQTSGRIVVAHR"
     gene            1031..2242
                     /locus_tag="VFU15_13490"
     CDS             1031..2242
                     /locus_tag="VFU15_13490"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HEU4718849.1"
                     /translation="MKKKSNKHPLKISVAEAKKIAAVSQGLAGLFSKGKPQDRTLSII
                     RHLGYVQIDTLSMVARAHHHTLFTRSKDYKEHHLDALMNEKKIFEYWSHAAAYLPMED
                     FRFSLPRKHDFRKGKAHWFARDKKMHRYVRDKIKAEGPLQAKDFGAERKHGSWFDWKP
                     AKIALEQLFQDGTLMIAGRKGFQKIYDLTERVLPAGVSDKLPSDEEYAEYLVRSALRA
                     HGIASQKDMTHLRRGMQEHALKKLKQLLREKEILEISVEGVKENYFVLPGTVAGIATF
                     PELTGTHLFSPFDNLIIRRERLKKIFNYDFQVEFYLPEHKRKFGYFCLPVLCGDEFIG
                     RMDVKADRSLKTFFIRSFHVENPAAVNENTWSAFAAAVKSFAAFNACEKIKVEKVYPG
                     KYRAELARALR"
     gene            complement(2250..2804)
                     /locus_tag="VFU15_13495"
     CDS             complement(2250..2804)
                     /locus_tag="VFU15_13495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718850.1"
                     /translation="MKKLFLFTLVLFSVAVNAQTRQSLLKSALVNSTAGDTFLLRDSV
                     LAHIYYDAESDSMLLAGLKYDGLSQADADYMRAQLMNYRPHTWTKDSITGAVIVPSSA
                     VPSAALEPKKSAKAWSKYFETHNKGYYEVSEPVFSRDGMYAVVYVAFECGANCGNGGA
                     TLYHFENGEWKPVSNLFSYNKQGK"
     gene            complement(2847..3371)
                     /locus_tag="VFU15_13500"
     CDS             complement(2847..3371)
                     /locus_tag="VFU15_13500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011460612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU4718851.1"
                     /translation="MLFSSARLSFRAFSETDIGAFARLNADPDVMRYFPSVMTKEESR
                     AAAKRITGHFEKYGYGYYSVFEKATEEFIGLIGFGHPQFESWFTPCVEIGWRMNKAFH
                     GKGYATEGALACFGYGKKNFALSTVFSFTAVINKPSENVMKRIGMEKEGGFDHPLIAE
                     SSPLRRHVLYRINM"
     gene            complement(3451..3843)
                     /locus_tag="VFU15_13505"
     CDS             complement(3451..3843)
                     /locus_tag="VFU15_13505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006417367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718852.1"
                     /translation="MRWFGRIILGALAFCAFALLFGWATMALWNWLMPSLFHLPVLTS
                     FWQAVGLIILGKLLFGGFGKHGCRHCGHRGWRHHRGHWRRRWEEKMANMTPEERERVM
                     KGMRHCDWWDQKWDDKNMGKDTADTPQQ"
     gene            complement(3913..4473)
                     /locus_tag="VFU15_13510"
     CDS             complement(3913..4473)
                     /locus_tag="VFU15_13510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013578639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU4718853.1"
                     /translation="MAAEQNRFLEETLKRERRRLLEFIRRRVPEDEDAEDILQDVFIE
                     LTENLRMDNVIEKVSGWMYRVARNKIADLFRRRRSESLEARYEKTNDDGEEGLGLNDL
                     LPDPSAGPEAQYARRVIMSELMEALDELPEEQRWVFWENEVEGRTFRELAEEAGVSLN
                     TLLSRKRYAVLFLRDRLKELYDDMNA"
BASE COUNT         1128 a         1242 c         1236 g         1157 t
ORIGIN      
        1 tcgataattc cagcatcagt gctgcgatcc agccggatgt gatttttgaa cccggcgttc
       61 agaaatacgc ggtttcttat tacgattcca cagcttcggc actcctgctt ttttccaccg
      121 gttacaatgt caatgcttct tcatcgtgga cgatagtgtc ggcccagtat aatgatgcaa
      181 ccaataacct gaaagcagcg catccgcaga ttgcatggaa cccgaatacg acttcagccg
      241 tatgtgcctg gaatgcggaa ggtgtttcaa ccaacggcgt atcgatgttc gatgcagaag
      301 tggtttccac gaatgtggat cagctgatcg ctgctgcgga ttttgtttcg ccttacccga
      361 acccttcttc ggggataacg acatttgagt tcaacctgaa agagcagcgc gatgttcagc
      421 ttcatgtgac gaacatgatg ggacaggaag tgatggcccc ggttgatatg ggcagcctgc
      481 ttcccggtaa ttatacgctg cccgtggatt tttccgcgct cagcaacgga acgtacatat
      541 ataccatgca ggtcggaaac gaacagacca gtggacgtat agttgtcgca caccggtaat
      601 ttgtttttta caggtgaaga cggggaactt gttccccgtt ttttttgcat tcccaaaaat
      661 tacttcagcg gaatggaatt ttacagcccg tcgcaaagca gtttttatgt ggtgtctcga
      721 ctcgcgctcg acacgacaag gtgatcagct ttatgtgtgg tgtcccgact cgcgtcggca
      781 cgacataaaa gcagctttat gtgtggtgtc ccgactcgcg cccgacacga cataaaagca
      841 gtttttgtat ggtgtcccgg ctcgcgctcg acacgacata aaagcagctt tatgtgtggt
      901 gtcccgactc gcgctcgaca cgacaaggtg atcagcgccg ggcgcttttt agcgcagctt
      961 ttctaaaaaa agttaccggg aaaaccaacc agatcgagga tgtattcccg taattttaac
     1021 gctcgccgaa atgaaaaaga aaagcaacaa acatccgctg aaaatttcag tcgcggaagc
     1081 gaaaaagatc gccgccgttt cgcagggact ggccgggctt ttttcaaagg gaaaaccgca
     1141 ggacaggacg ctttccatta tccgccatct cggctacgtg cagatagata cattgtcgat
     1201 ggtggcgcgt gcacatcatc atacactctt cacgcgatcg aaagattaca aagaacatca
     1261 cctcgatgcg ctgatgaacg aaaaaaagat cttcgaatac tggagccacg cagcggcgta
     1321 tctcccgatg gaagatttcc gtttcagtct tccgcgcaag catgatttcc ggaaagggaa
     1381 agcacactgg ttcgcacgcg ataaaaaaat gcaccggtat gtccgcgaca aaataaaagc
     1441 ggaaggtccg ctgcaggcga aagatttcgg agcggagcgg aaacacggga gctggttcga
     1501 ctggaaaccg gcgaagatcg cgctggaaca attgttccag gacggaacgt tgatgatcgc
     1561 cgggcgaaaa gggtttcaga aaatttacga cctcaccgaa cgcgttttgc ccgccggcgt
     1621 cagcgacaag cttccttcgg atgaggaata tgcggaatac ctggttcgct ctgctttgcg
     1681 cgcgcacggc atcgcttcgc agaaagacat gacgcatctc cgccggggca tgcaggagca
     1741 tgcgctgaaa aagctgaagc agctgctgcg tgaaaaggag atcctggaaa tttccgtgga
     1801 aggtgtgaag gagaattatt tcgtgttacc tggaactgtc gccggcattg cgacgttccc
     1861 ggagctgaca ggaacgcacc tgttttcgcc tttcgataat ctcattatcc gccgcgagcg
     1921 gctgaagaaa attttcaatt atgatttcca ggtggaattt tatctgccgg aacacaagcg
     1981 gaaattcggt tatttctgcc tgcccgtttt atgcggcgac gaattcatcg gccggatgga
     2041 tgtgaaagcc gaccggtcgc tgaaaacgtt tttcatccgg tccttccacg tggaaaaccc
     2101 ggcggctgtg aatgaaaaca catggagcgc gttcgcggct gccgtgaaaa gtttcgctgc
     2161 attcaatgcc tgcgaaaaaa taaaagtgga gaaagtgtat cccgggaaat accgggcgga
     2221 gctcgcgcgg gcgctgcgtt aacccggagt tattttccct gcttgttgta ggagaaaagg
     2281 ttgctcaccg gtttccattc gccgttctcg aaatgataga gcgttgcgcc cccgttgccg
     2341 cagttcgcgc cgcattcgaa ggcaacgtac acaacggcgt acatcccatc ccgcgaaaaa
     2401 acgggttcgc tcacttcgta ataacctttg ttgtgcgttt caaaatattt cgaccatgct
     2461 tttgccgatt ttttcggttc gagcgcagct gagggaacag cggaagaggg gacgatcacc
     2521 gcgccggtaa tggaatcttt tgtccacgtg tgcggcctgt agttcatgag ctgtgcacgc
     2581 atgtaatccg catccgcctg cgagagcccg tcgtatttca gtccggcgag cagcatagag
     2641 tccgattccg catcgtaata aatatgcgcg agcacgctgt cgcggagcag gaacgtgtcg
     2701 cctgcggtgg aattgacaag cgcggatttg agcagcgact ggcgggtttg tgcatttacc
     2761 gcaacggaga acagcacaag ggtgaagagg aagagttttt tcatggaagg gtatgagcgg
     2821 caaatttaca aactacatgc cacgaatcac atgtttattc tgtacagcac gtgacgccgt
     2881 aagggggagc tttccgcgat cagcgggtgg tcgaacccgc cttctttttc catcccgatg
     2941 cgtttcatga cattttcaga aggtttattg atcacggcgg taaacgaaaa gaccgtgctc
     3001 aatgcgaaat tctttttccc ataaccgaaa caagccagtg cgccttccgt cgcataacct
     3061 tttccatgga acgccttgtt catccgccag ccgatctcca cgcagggcgt aaaccagctt
     3121 tcaaactgcg ggtggccaaa accgatcagc ccaatgaatt cttccgttgc tttttcgaag
     3181 acggaataat agccgtaacc gtatttttca aaatgaccgg taatacgttt tgcggccgcc
     3241 cggctttcct cttttgtcat caccgaaggg aaataacgca tcacatccgg atcggcattg
     3301 agccgcgcga acgcgccgat atccgtttct gaaaatgcgc ggaaggaaag gcgggccgag
     3361 gaaaagagca tggcggtgcg aaattacggg aataaaaaaa cgccccggaa aaatccccgg
     3421 gacgttcaat caacatcaac ccaactccgc ttattgctgc ggcgtatccg cagtgtcttt
     3481 tcccatgttc ttgtcgtccc atttctggtc ccaccagtcg caatggcgca tgcctttcat
     3541 cacgcgttct ctttcttccg gcgtcatgtt tgccattttt tcctcccagc gcctgcgcca
     3601 gtggccccgg tggtgtcgcc acccgcggtg accgcaatgc ctgcagccgt gttttccgaa
     3661 tccaccgaag agcagcttgc cgaggataat cagtccgacc gcctgccaga agctggtcag
     3721 cacgggaaga tgaaaaagcg acggcataag ccagttccac agcgccatcg tagcccagcc
     3781 gaacaggagt gcgaatgcac agaaggccag ggcgcctaaa attattctac cgaaccatct
     3841 catgttttta gttttttgta ttcagtttgt tttttgagtt ctgtttctga aggtcagctg
     3901 ttccggttct ccttacgcat tcatgtcgtc gtacagttct ttgagcctgt cgcggagaaa
     3961 aagaacggcg tacctttttc gtgataaaag agtgttgagc gaaactccgg cctcttcggc
     4021 cagttcacgg aacgtacggc cctctacttc attttcccag aacacccaac gttgttcctc
     4081 gggcagctcg tccagtgctt ccatcagttc actcatgatc acgcggcgtg catactgcgc
     4141 ttccggtccc gccgaaggat cgggcagaag atcgttcagc ccgaggcctt cttccccgtc
     4201 atcgtttgtt ttttcgtaac gggcttccag cgattcgctt ctgcgcctgc ggaaaagatc
     4261 cgcgatcttg ttgcgcgcca cacggtacat ccaccccgac actttctcga tcacattatc
     4321 catacgcagg ttctcggtta gctctatgaa aacgtcctgc aggatgtctt ctgcgtcttc
     4381 atcttcggga acgcgtctgc gtatgaattc cagcaaccgc cgcctttcgc gcttgagcgt
     4441 ctcttcgaga aaccggtttt gttcggcggc cattaatgtc ggagtgttaa ccatttcctt
     4501 catctgtatg ggatgtcgga acgggcgggt gaattatttt acaaaagtgg gaaaaagccg
     4561 cttccggggt cagatttcgg tacgcaggct actcaatctg accccgtgga gccccgtgta
     4621 tcaaaatcgg aacggaaaag cagatccctg gctttcgtgt ggaaaggcgc aattcgttgg
     4681 tgggaaaaac tcttcaccgg tcagattgag tagccccgcg tatcgaaatc tggccgatga
     4741 agtttccccg gctattcaca att
//
LOCUS       DASTSF010000466         9763 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1189275,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000466 DASTSF010000000
VERSION     DASTSF010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 9763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9763
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1189275"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /locus_tag="VFU15_13515"
     CDS             <1..295
                     /locus_tag="VFU15_13515"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HEU4718854.1"
                     /translation="SIDYIISSPPVVPTAFSPNGDGQNDYFIVRGGPFKELELRIYNN
                     WGELIYIGTTQYPGWDGTRDGVPQAIGVYVYTVHAVTPDDHAYDLSGDVTLLR"
     gene            312..1313
                     /locus_tag="VFU15_13520"
     CDS             312..1313
                     /locus_tag="VFU15_13520"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HEU4718855.1"
                     /translation="MKTSFFRNALSAALLAASAFSLRAQDFHLTHYDAAKIYLNPAMT
                     GMFDGYYRIHANYRNQWSAVATKPFQTEALAFDMPIKRYGVGVQLMNNRAGFGNFDVL
                     QLNFSGAYDLQCDKNNNHHVAFGMEAGVMQKSVDFGRLSWDNQYTMADGGGFDASLPS
                     GESYDTERNYLLDISAGALYYYAKEASRINPFFGFSVFHLNQPRESFYGTSNRLPLHY
                     IIHTGARIGINERLQLQPRFLYMWQLNDHEMVLGLLANIMLPNSDYLIVGPSFRTKDA
                     GIMEVGVKHGAFTYRMSYDVNVSTLRPATNERGGIEFSVIYIAKRKVPVVAPNCPRL"
     gene            1310..3460
                     /locus_tag="VFU15_13525"
     CDS             1310..3460
                     /locus_tag="VFU15_13525"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEU4718856.1"
                     /translation="MRRFALLLFSLFSLPVLLSAQSRREWQDMGDKAFDQKNYKLALA
                     CYQHVITMTPGNDRDLVTPYECRSYVPAKKLVDTTGISGAMMDTTLKNPNLVSGNDAR
                     TRWVVHRVADCYRLLHDYDNAELWYRQAMFMPDKRFPDTQFWYANALMNNAKYEAAMK
                     QFDLYSEALDVMNPNYARATRCSAGCMFAMDTANFKKEVIVTKMDSVVNAGTAVFSAT
                     YYGDPQTVLYTAAKSGNQSTPDLVKLGLDAYTTDIYTAQLNNGHWQNPQPVTGGVTPL
                     NTPMNEGAAMVSFGKANLFFTRWSDDGKECAIYMCKNVDDKWLAPKKLDANVNQPGCR
                     NMMPFLSNDGTTLYFVSDRAGGQGGLDIWTVQIDDDGNCSAAKNLGPVINTPDDEITP
                     FYHFTSSTLFYSSNGMGGYGGFDIVKSSFNAEDSTWSQTKNLDAPFNSSRDDAYFVLD
                     RTQQQGYFSSDRDPCQACGPGGGYCYRAYQFENEPLIFSIHGHVYDAATNQPISNALL
                     TFKDIRGVSPDTYAFTDSTGYYYADLSLGQELYIKAQKNKYFGDATNISTVGLTDSKV
                     FEHDFFLTQIPAGDIVIPGIEYDFDKATLRPESMKVLDDLAAFLILNDNISVEIAAHT
                     DSRGSDSYNLDLSQRRAQSCVDYLISKGISRDRLVAKGYGETVPLITDAEINKMATKD
                     EREAAYQKNRRTTFRPIKEGVIMDKWEGKKPGDN"
     gene            3484..4137
                     /locus_tag="VFU15_13530"
     CDS             3484..4137
                     /locus_tag="VFU15_13530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718857.1"
                     /translation="MKLFNPVFVSLFSFFIFVSNLEAQGNIEGGLYTEYSQFGYYGQF
                     AAGGVLYIPWKDRFTLNYEACIGRQNNRGFYAHAPAGALSGAYLIGQASGVTFLNYLG
                     VLLFLVPEGVGMYVTEGKISTHISVNPLGFDYWYKRDPYDETGCLSGSIVFRLKMPLQ
                     GYSWPLFVAPQVAVTWLYGNSDPVGNPGLRAGVCIGFDAKNNPFRDDPFLDDPVLRH"
     gene            4227..4625
                     /locus_tag="VFU15_13535"
     CDS             4227..4625
                     /locus_tag="VFU15_13535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEU4718858.1"
                     /translation="MLWQAGNAWQRELKTALEPVGITHVQFLLLETLEELQSSGIPAT
                     QSRLAVASGSDVMMTSKVLRLLERKKLVGRKSYAKDARANSIVLTPEGAKVLAKAKKI
                     VTANEERFFAKLVTKPGKFVQNLEALAEGK"
     gene            complement(4626..6185)
                     /locus_tag="VFU15_13540"
     CDS             complement(4626..6185)
                     /locus_tag="VFU15_13540"
                     /inference="COORDINATES: protein motif:HMM:NF027446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4836 family protein"
                     /protein_id="HEU4718859.1"
                     /translation="MRKFLVIAAFSIVFLAGIAMLAWFFFIRRSLPEYASCIPSDSFL
                     VVTLNTKNLAVDQAKGGHLFPEFAIDKKQPALLKEIRNAVERSGGSGIKLSSDVLGFV
                     YRENETAFWGACFAVDDSAQAAKFVRQSLLRDFPVHIIDGGRKNMFGLDSSSAVFSWN
                     RKTFLVLYPVSDNDSAVVAAQCEKLLAQDKKHSLLSNPGFREHELSSFDIGVWIQAKP
                     LLEFTNGGSLFTTMLFGVDNISLQAEFNDGEIDVRRTVKLNEDGNKSPVRGNTMLLSC
                     RPEEVKGFLHTPLNLDDTVIKAEMKMPPLKYLPFTDEQNKELFQQLDGNAWMLFHDTI
                     SFPSDYITYEYDEDFRQVPVTKTVMNNQRSLSYCFGLKNADNVRSLVRKWMAEDTVKM
                     EGNYWRIGGTNPAQRLFVTPTALVITDWPSYDGKDRPAPAEWYGKEMLIPAGEFATGP
                     DMLGLSFLFPKIDRLEGILSGCIGDLTLSTPLQTSAGSSSEFRLVFTERKINALIQAE
                     EIVLKSIGDGN"
     gene            complement(6182..8233)
                     /locus_tag="VFU15_13545"
     CDS             complement(6182..8233)
                     /locus_tag="VFU15_13545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718860.1"
                     /translation="MKKFFLLFIFLLPAIVFAQDNSGHIPAGSNYIVTINPSAHIGNG
                     DFSALGKLEMFTHSGETDENGNSLFFGDDDLDADRKAAFDGFFTTIFAQPSKAGIDTT
                     KKFFIFFNSPDSVHYWAYVLPLSNADVFGDFMLSSLFSEKPAVDKGSGFSMVNAERLS
                     VGWTGSYAIILLADYDFYIDETGKNPVEQQHIRDSIMLANMYGIGNSSTDSVISDSLR
                     AQRTARLQKEEKEMLEKLAKDTTPEENVEVRYSYDSYYNPGDYDVPEVDKDSLVRKAA
                     RNEMKRLINLPYDLSVCSVANFRNVQQEDFDAMYWYNYGDVMQKRYEEQMSLRRDYYY
                     YTNIGPDTSNIQNMWKGSYIASIVKISGNVATMEHRAYFSPALQANTKGMYTGRVKRK
                     MFRYVKGENMLGFTAMSVDMEKFMKFYGSVYRESMSNSFAGMYQSYYLMVWDLLRVFL
                     DDKTMYNLFDGKFLFAVTDLKPFTSSYVTYEYDENFNQQEVRKEKTEIRPEFVFIAGL
                     GKEKKAKQILAILEKANAVKKQNPMYYLINTPGEYDIKMYLAIRNGMLIITNNEDLIQ
                     NHLKHGYSRKERVKGKIRRTGRRSPIVGWWDGKKSFALIKKNEEGNLSDDDLKSLDLL
                     EKNISTGLIIGRRPKNGVQRIDVKVEMNSDDGKSGQTNFVRFFRMINSLFLIHSQTGS
                     Q"
     gene            complement(8243..8926)
                     /locus_tag="VFU15_13550"
     CDS             complement(8243..8926)
                     /locus_tag="VFU15_13550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718861.1"
                     /translation="MYGKRPAPVFLLFFAAIAIVFSGCFEIVEDVTIHSNGSGTFFYT
                     VNLSQSKVRLDGIMQLDSTDGYRVPKKDEINGDILKAKSTLEKSPGITNVETTSDWTN
                     YIFTMKFDFSSVNNLNLAVESVSQELTTDHKRIPEASDNFAFAGKSFDRQSNYDAKVQ
                     GPKLTPKDKEILKDATYTCIYRFDDAVKSCSNTDSKISKSGKSVMLKLNMLQLALGQK
                     SIVNRISLQ"
     gene            8984..9721
                     /locus_tag="VFU15_13555"
     CDS             8984..9721
                     /locus_tag="VFU15_13555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012821207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEU4718862.1"
                     /translation="MFRRDPFGHFLFLKRKLISLIGWLTWRRYNRRNRLEISGTEILA
                     NLPERNVMFISNHQTYFADVIAMLHVFCAVRNGYHNSTSKPWYLFRPKINNYFVAAEE
                     TMKKGILPRIFAYAGSISIQRTWREGNQDINRKVRMEDLSNIGIALSDGWLINFPQGT
                     TKPGAKGRRGITILVKKYNPVIVPVVIDGFRRAFDKKGLKLKKTGVTLKMRFKEPMNI
                     DPGESSEMILEKMMEAIEQLPEAAVKP"
BASE COUNT         2315 a         2633 c         2531 g         2284 t
ORIGIN      
        1 gtctatcgat tacatcatca gctctccgcc ggtcgtaccg acggcattct ctcccaacgg
       61 agacgggcag aacgattatt tcattgtgcg cggaggaccg ttcaaggagc tcgagctgag
      121 gatctacaac aactggggcg agctgatcta catcggcacg acacaatacc cgggatggga
      181 cggcacgcgc gacggagttc cgcaggccat cggcgtttat gtttacaccg ttcacgctgt
      241 aacgccggat gatcatgcct atgatctttc aggtgatgta accctattac gataacccag
      301 gcgagaaaac catgaaaaca agttttttca gaaacgcgct ttccgcagcc cttctcgcgg
      361 cgtccgcatt ttccctgcgt gcgcaggatt ttcacctgac gcactacgat gccgccaaga
      421 tctacctcaa cccggcgatg acgggaatgt tcgacggtta ttaccgcatt cacgcgaatt
      481 acaggaacca gtggtcggct gttgcgacga aacctttcca gacggaagcg ctcgcgttcg
      541 acatgcctat caaacgttac ggtgtgggcg tccagctgat gaacaaccgc gcgggcttcg
      601 gaaatttcga tgtgctccag ctgaattttt ccggcgcgta tgatctccag tgcgacaaga
      661 acaacaacca ccacgtcgcg ttcgggatgg aagccggcgt gatgcagaag agcgtcgatt
      721 tcggcagact ttcctgggat aaccaatata cgatggcgga cggcggcgga ttcgacgctt
      781 cccttccgag cggagagagc tacgataccg aacggaatta cctgctcgat atttctgcag
      841 gcgcgttgta ttattatgca aaggaggcga gccgcatcaa tcctttcttc ggcttttctg
      901 tttttcacct caaccagccg agggaatcgt tttacggcac gagcaaccgt cttccgctcc
      961 actacatcat ccacaccggt gcgcgcatcg gcatcaatga acggctgcag cttcagccgc
     1021 gtttccttta catgtggcag ctcaacgacc atgaaatggt gctcgggctc ctggccaaca
     1081 tcatgctgcc gaattccgat tacctcatcg tcggtccttc cttccggaca aaagatgccg
     1141 ggatcatgga agtcggcgtg aagcacggcg cgttcaccta ccgcatgagc tatgacgtaa
     1201 atgtttccac gctgcgtcct gctaccaatg agcgcggcgg gatcgaattt tccgtgatct
     1261 acatcgccaa acgaaaagtt cccgttgtcg cacctaattg tccgagacta tgaggcgctt
     1321 tgcactcctt cttttttcgc tcttttccct tcccgttctt ctttctgcgc aaagccgcag
     1381 ggaatggcag gacatgggcg acaaagcgtt cgaccagaaa aactacaagc tggcgctggc
     1441 ctgctaccag cacgtcatca cgatgacgcc gggcaacgac cgcgatctcg tcacacccta
     1501 cgaatgccgt tcatacgttc ctgcgaaaaa gctcgtcgac accactggca taagcggtgc
     1561 gatgatggac acaaccctga aaaatccgaa cctcgtttca ggaaatgacg cacgcacgcg
     1621 atgggtggtt caccgcgttg ccgactgtta ccgcctgctc catgattacg acaacgcgga
     1681 gctctggtac cggcaggcga tgttcatgcc cgataaacgt ttcccggata cgcagttctg
     1741 gtatgcaaat gcattgatga acaatgcgaa gtacgaggcc gccatgaagc agttcgatct
     1801 ctattcggaa gcgcttgatg tgatgaaccc gaactacgcg agagccacgc gttgcagcgc
     1861 aggatgcatg ttcgcgatgg atacggcgaa tttcaagaag gaagtgatcg tgacaaaaat
     1921 ggacagcgtg gtgaatgcag gcaccgctgt tttttctgca acgtattacg gagatccgca
     1981 gacagttctt tatacagctg caaaatccgg taaccagtcg acacccgatc tcgtgaagct
     2041 cgggctcgat gcatacacca cggacattta caccgcacag ctgaacaacg gccactggca
     2101 gaatccgcag cctgtaacgg gcggcgtgac tccgctcaac acgccgatga acgaaggtgc
     2161 ggcgatggtt tccttcggga aagcaaatct tttcttcacg cgctggagcg acgatggaaa
     2221 agaatgcgcc atctatatgt gcaagaacgt cgatgataaa tggctcgcgc cgaaaaagct
     2281 cgacgcgaac gtcaaccagc cgggatgcag gaacatgatg ccgtttcttt cgaacgacgg
     2341 aacgacgctt tattttgttt cggaccgcgc gggcggacag ggcgggctcg acatctggac
     2401 ggtacagatc gacgatgacg gaaattgttc cgcagcaaaa aacctcggac ctgtcatcaa
     2461 cacgccggac gatgagatca cgcccttcta tcattttacg tcgagcacat tgttctacag
     2521 ctcgaacgga atgggcggct acggcggatt cgacatcgtg aagtcgtcgt tcaacgcaga
     2581 agattccacg tggagccaga caaaaaatct cgacgcgccg ttcaactctt cccgcgacga
     2641 cgcttatttc gttctcgacc gcacgcagca gcagggttat ttttcttcag accgtgatcc
     2701 gtgccaggca tgcggacccg gcggcggcta ctgctaccgt gcgtaccagt tcgaaaacga
     2761 gccgctgatc ttttccattc acgggcacgt ttatgatgcg gccaccaacc agccgatcag
     2821 caatgcgctg ctcacgttca aggatatccg cggcgtttca ccggacacgt acgcattcac
     2881 cgattcaacc gggtattatt acgcagatct ttcgctcggg caggagcttt acatcaaggc
     2941 gcagaagaat aaatacttcg gcgatgcgac gaatatttcc acggtcggac ttaccgacag
     3001 taaagtgttc gagcacgatt tcttccttac gcagattcct gcgggcgata tcgtcatccc
     3061 gggaatcgaa tacgatttcg acaaggcaac gctgcgtccc gagtcgatga aagtgcttga
     3121 cgatcttgcc gcgttcctca tcctgaacga caacatcagc gtagagatcg cggcgcacac
     3181 cgattcgcgc ggaagcgatt cgtacaacct cgatctttcc cagcgccgtg cgcaaagctg
     3241 cgtcgattat ctcatctcga aaggtatttc ccgcgaccgg cttgtcgcga agggatatgg
     3301 agaaaccgtt ccgctcatca ccgatgccga gatcaacaag atggcgacga aagacgagcg
     3361 cgaagccgct taccagaaaa accggagaac cacgttccgt cccatcaagg aaggtgtgat
     3421 catggacaaa tgggaaggaa aaaaacccgg ggacaattaa gttcagttcc acgtccgtaa
     3481 tgaatgaagc tttttaatcc cgttttcgta tcgctttttt cttttttcat cttcgtttca
     3541 aaccttgaag cgcaggggaa tattgaaggc gggctataca cagaatattc ccagttcggc
     3601 tattacggcc agttcgctgc aggcggtgtt ctttacattc cgtggaaaga ccgttttacg
     3661 ctgaattacg aagcgtgtat cggccggcag aacaatcgtg gtttttacgc gcatgcaccg
     3721 gccggggcac tgtcaggcgc atacctgatc ggccaggctt ccggtgtcac tttcctcaat
     3781 taccttggtg tcctgctgtt ccttgtaccg gaaggtgtgg gcatgtatgt aacggaagga
     3841 aagatctcta cccacatttc cgtcaatcca ctcggtttcg actactggta caaacgcgat
     3901 ccttacgacg agacaggatg cctttcaggg agcattgtgt tccgtctcaa aatgccgttg
     3961 cagggctaca gctggccgtt gttcgttgcg ccgcaggtag cggtgacgtg gctttacggg
     4021 aatagtgatc ccgtcgggaa tccggggttg cgcgccggcg tctgcattgg tttcgatgca
     4081 aaaaacaacc cgtttcggga tgatccgttc ctggatgacc cggtcttgcg ccattaaaat
     4141 tattagggct atttttgtcc ggtaaatccc cctacaccat gtcttcaaaa aagaaatcca
     4201 ggaaaaattt acgtaaaacc ggctttttgc tctggcaggc cggtaacgcc tggcaacgtg
     4261 aactcaaaac cgcccttgag cccgtcggca tcactcacgt tcagttcctg ctgctcgaaa
     4321 cactggaaga attgcagtcc tcgggaattc ccgccacgca atccaggctg gcggtcgctt
     4381 caggatcgga tgtcatgatg acgtcgaaag tgcttcgcct gctggaacgg aaaaagctcg
     4441 tcgggcgaaa atcctatgcg aaggacgcac gtgccaattc catcgtgctt acacccgaag
     4501 gcgcgaaagt gctggcaaaa gcaaagaaga tcgtaaccgc caacgaggaa cgtttttttg
     4561 caaagctggt caccaaaccc ggaaaattcg ttcagaacct ggaagccctg gcggaaggga
     4621 aataatcagt tcccgtcacc gatcgatttc agcacgatct cttccgcctg gatgagggca
     4681 ttgatctttc gttccgtgaa gacaagcctg aactccgaag aagagcccgc agatgtctgc
     4741 agcggcgtgg acaatgtcag gtcaccgatg cacccggaaa gtattccttc cagccggtca
     4801 attttcggaa agaggaacga aaggcccagc atatccggac ccgtagcaaa ttcacctgcg
     4861 gggatcaaca tttcctttcc gtaccattcc gccggcgccg gcctgtcctt gccatcgtac
     4921 gaaggccagt cggtgatgac cagcgcagtg ggtgtcacga aaagccgctg cgcaggattc
     4981 gttcccccga tgcgccagta atttccttcc attttcacgg tgtcttccgc catccatttc
     5041 ctgacgaggc tgcgaacgtt gtccgcgttc ttcaacccga agcaatagct cagggaacgc
     5101 tggttgttca tcacggtttt cgtaacgggc acctggcgga aatcctcatc gtattcgtac
     5161 gtgatgtaat cggacggaaa agagatcgtg tcatggaaca gcatccatgc gtttccgtcg
     5221 agctgctgaa aaagttcttt gttctgctcg tcggtaaatg gcagatattt cagcggcggc
     5281 attttcattt ccgctttgat cacggtgtcg tcgaggttga gcggcgtatg caggaatcct
     5341 ttcacttctt ccgggcggca ggaaagcagc atcgtattgc cgcgaaccgg cgatttgttc
     5401 ccgtcctcgt tcagcttcac cgttctccgc acgtcgatct caccgtcatt gaattcagcc
     5461 tgcagcgaaa tattatccac accgaaaagc atcgtcgtga acaggcttcc cccgttggta
     5521 aattccagca agggtttcgc ctgtatccat acaccgatat cgaacgacga caattcatgc
     5581 tcgcggaagc cgggattcga cagcagcgaa tgttttttgt cctgggcgag cagcttttca
     5641 cattgcgcag caaccaccgc agaatcgtta tcggaaacag gatacagcac gaggaatgtt
     5701 ttccggttcc agctgaaaac ggccgaagat gaatcgaggc cgaacatatt cttccgtccg
     5761 ccgtcaatga tgtgaacggg aaaatcacgc agcagactct ggcgaacaaa tttcgcggcc
     5821 tgtgcgctgt catcgaccgc gaagcacgcg ccccagaaag cggtttcatt ttcacggtaa
     5881 acaaaaccga gcacgtcgga ggaaagcttt atgccggaac cgccgcttcg ctccaccgcg
     5941 ttcctgatct ctttcagcaa tgccggctgt tttttgtcga tggcgaattc gggaaaaaga
     6001 tgcccgcctt tcgcctggtc gaccgcgagg ttcttcgtat tgagcgtaac gacgaggaac
     6061 gaatcggaag gaatgcacga cgcatattcc ggaagcgacc gccggatgaa aaaaaaccag
     6121 gcaagcatgg cgatgccggc caggaaaaca atgctgaacg ctgcgatgac caggaatttc
     6181 ctcactgcga accggtttgc gaatgaataa ggaaaagtga gttgatcatg cggaagaaac
     6241 gcacaaaatt cgtttgcccg ctcttcccgt cgtcgctgtt catctccact ttcacgtcga
     6301 tgcgctgtac gccgttcttc ggccgcctgc cgatgatgag ccccgttgaa atatttttct
     6361 cgagaagatc gagcgatttc aagtcgtcat cggacaggtt gccttcttcg ttcttcttga
     6421 tcagcgcgaa agattttttt ccatcccacc agccgacgat cgggctgcgg cgtcctgtcc
     6481 gccggatctt ccctttgacg cgttccttcc ggctgtagcc atgcttcagg tgattctgga
     6541 tcaggtcttc attgttcgtg atgatcagca tcccgttgcg tatggcgaga tacattttga
     6601 tgtcgtattc gccgggcgtg ttgatgaggt aatacatcgg gttctgcttc ttcacggcgt
     6661 tcgccttttc aagaatggcg agtatctgtt ttgctttttt ctctttcccg aggccggcga
     6721 tgaaaacaaa ttccgggcgt atctccgtct tttccttccg cacttcctgc tggttgaaat
     6781 tctcatcgta ttcgtacgtg acgtaagacg aagtgaacgg cttaagatcc gtcaccgcga
     6841 aaaggaattt cccgtcgaaa agattgtaca tcgttttatc gtcgagaaaa acacgcagca
     6901 ggtcccacac catcaggtaa tacgactggt acatcccggc gaatgaattg ctcatcgatt
     6961 cccggtaaac ggatccgtag aatttcatga acttttccat gtccaccgac atggcggtga
     7021 agccgagcat gttctcgcct ttcacgtaac ggaacatttt ccgcttcacg cgcccggtgt
     7081 acatgccttt cgtattcgcc tgcagcgccg gagaaaaata cgcgcggtgc tccatcgtcg
     7141 cgacattccc gctgatcttc acgatgctcg cgatgtagga acctttccac atgttctgaa
     7201 tgttgctggt atccggaccg atgttggtgt agtaataata atcgcggcgc aggctcatct
     7261 gctcttcgta gcgtttctgc atcacatcgc cgtagttgta ccaatacatc gcgtcgaaat
     7321 cttcctgctg cacattccgg aaattcgcca cggaacaaac cgacagatcg tatgggagat
     7381 tgatcagccg cttcatctcg ttgcgcgcag ccttgcgcac gagcgaatcc ttgtccactt
     7441 ccggaacatc ataatcaccg ggattgtaat agctgtcgta agaatacctg acttcgacgt
     7501 tctcttccgg cgtcgtatcc ttcgccagct tttcaagcat ttccttttcc tctttctgca
     7561 ggcgcgcggt ccgctgcgca cgcagggaat cggagatgac cgagtccgtt gacgaattac
     7621 cgattccgta catgttggcg agcatgatgc tgtccctgat gtgctgctgc tccaccgggt
     7681 tcttccctgt ctcatcgata taaaaatcat aatccgcgag gaggatgatc gcgtaggaac
     7741 cggtccaccc gacggaaagg cgttccgcat tcaccatgga aaaaccggag cctttatcga
     7801 ccgcaggttt ttcagaaaaa aggcttgaca acatgaaatc gccgaataca tccgcgttgc
     7861 tcagcggaag aacgtacgcc cagtaatgaa cggaatcggg actattgaag aagatgaaga
     7921 actttttcgt ggtgtcgatc cctgctttgg aaggctgcgc gaagatcgtc gtgaagaagc
     7981 cgtcgaacgc ggctttgcgg tccgcatcga ggtcgtcgtc accgaaaaaa aggctgttgc
     8041 cgttttcatc cgtttccccg gaatgcgtga acatctcgag ctttccgagc gcggaaaaat
     8101 cgccgttccc gatgtgcgcg gaaggattga tggtaacgat gtaattcgac ccggccggga
     8161 tatggccgga attatcctgt gcgaaaacga tcgccggcaa caggaaaatg aaaagaagaa
     8221 agaacttttt cataggggtt atttactgga gcgaaatacg gttgacgatg ctcttctgcc
     8281 cgagcgccag ctgcagcatg ttcagcttca gcatgaccga tttcccgctt ttcgagattt
     8341 tcgaatcagt gttcgagcag gatttcaccg catcgtcgaa gcggtagatg caggtgtagg
     8401 ttgcatcttt caaaatttcc ttgtctttcg gtgtgagctt gggtccctgc actttcgcat
     8461 cgtaattgct ctgccggtcg aatgatttcc cggcgaaggc gaaattatcg gaagcttccg
     8521 ggatgcgttt gtgatcggtg gtcagctcct gcgaaacaga ttccacggca aggttgaggt
     8581 tgttgacgga actgaaatcg aacttcatgg tgaagatata gttcgtccag tcgctggtag
     8641 tttccacgtt cgtgattccc ggggattttt ccagcgtgct tttcgctttg aggatatcgc
     8701 cgttgatctc gtcttttttg ggaacgcggt aaccgtcggt gctgtcgagc tgcatgatgc
     8761 cgtcaagacg cactttgctt tgcgaaagat ttacggtata gaaaaaagtt ccggaaccgt
     8821 tgctgtggat cgtcacgtcc tcgacgatct cgaaacagcc ggaaaacacg atagcgatcg
     8881 cagcaaaaaa gagaagaaaa accggggccg gacgttttcc gtacatagca gtaaaggtaa
     8941 tgaaacggga ggcttaactc atgaatgctt acttttgaaa accatgttcc gcagggatcc
     9001 gttcggacac ttcctttttc tcaaacgtaa actcatcagc cttatcggct ggctcacgtg
     9061 gcgacgctat aaccggagga acaggctgga aatttcgggc acggaaatac tggctaatct
     9121 ccccgagcgg aacgtgatgt tcatttccaa tcaccagacc tatttcgcgg acgttatcgc
     9181 catgctgcat gttttctgcg cggtcaggaa cggctatcac aactcgacgt cgaagccctg
     9241 gtatcttttc cggccgaaga tcaacaacta cttcgtagcc gcagaagaga caatgaaaaa
     9301 aggaatcctg ccgcgcattt tcgcgtatgc aggatcgatc tccatccagc gcacgtggag
     9361 agaggggaac caggacatca accggaaagt gcggatggaa gacctgtcca acatcggcat
     9421 tgcgctcagc gacggctggc tgatcaattt tccgcagggc acgaccaaac ccggtgcaaa
     9481 aggccgccgc ggcatcacga tcctcgtcaa aaaatacaat cctgttatcg ttcccgtggt
     9541 gatcgacggt ttcaggcgcg ctttcgacaa gaaaggactg aagctgaaga aaacgggggt
     9601 tacgctgaag atgcgtttca aggaaccgat gaacatcgat cccggggaaa gctccgaaat
     9661 gatcctggaa aaaatgatgg aagccatcga gcagcttccg gaagcagccg tgaagccgtg
     9721 aacaacgccc gccgggcata aaaaaactcc cggcaaaacc ggg
//
LOCUS       DASTSF010000467         6594 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_41512,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000467 DASTSF010000000
VERSION     DASTSF010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 6594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6594
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41512"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(208..858)
                     /locus_tag="VFU15_13560"
     CDS             complement(208..858)
                     /locus_tag="VFU15_13560"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014202950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HEU4718863.1"
                     /translation="MKSVEELNNVYASLRPLQRIEKLYSDSEKVLLTSSFGTTSVILL
                     HMFHEVNPAQEIIFLDTTYHFAETLRYKEMLTERFGLNVRTAQPEEWKNAFTNSDQTW
                     SKDPDLCCSINKVEPLDKLKAGYEVWVSGLMQSQNNYRKQMTIFEQKETLLKFYPIID
                     LSEEEAKKYIAENNLPPHPLVAHGFNSVGCIHCTVKGRGREGRWVNRNKTECGLHI"
     gene            complement(855..2300)
                     /locus_tag="VFU15_13565"
     CDS             complement(855..2300)
                     /locus_tag="VFU15_13565"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HEU4718864.1"
                     /translation="MKLEDLRLLIIGGGNVALEKLNAVLSNSPATNVTLIAPEIRHEI
                     LELAKEFSSVIIFEREVSPQDFDEADIVFIAVNDFVLAEELVIEAHRRNLLVNVADTP
                     GFCDFYLGSIVQKGDLKIAVSTNGKSPTFSKRMKEVLNDSIPDETQVTIAHLHELRKH
                     LKGNFREKVEALNRATAVLVEKDAKKKFTGLKLRSWLFYSFSAISLMILGHLLFTYVP
                     FERIGSSITGSLSSIDSRILIWIAIGMGAQLVDGALGMAYGVTTTASLLSFGIPPAVA
                     SASMHASEIMLTGTSSLVYLKYRNINGKLFRSLLVPGAIGAILGACTISLLKEYVHYV
                     KPVISVYTLVLGVLILRKAIMEKKKKRSKIRRVGVLASFGGFLDSIGGGGWGPIVTST
                     LLAGGRDLRYSIGSAHAAKFFVALISSVTFFFMIGFAHWTIILGLIIGGMVAAPFSIW
                     LSTRISVRNGLALVGLLVIITSLKTIISALL"
     gene            complement(2428..3201)
                     /gene="cobA"
                     /locus_tag="VFU15_13570"
     CDS             complement(2428..3201)
                     /gene="cobA"
                     /locus_tag="VFU15_13570"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007804953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HEU4718865.1"
                     /translation="MSTQNIQPKVTLVGAGPGDPDLLTIKGVNALGEADVVLYDALVN
                     PEILKYAPADAKKVFVGKRSGNHSFTQEQINTLIVDYAFTHGHVVRLKGGDPFVFGRG
                     TEELEYAETFNIETDIVPGISSSTGVPALQRIPVTHRGTSESFWVITGRNSEGKVPED
                     IKLAARSGATVVILMGLRNLEKIVRIYQEHERGNLPVAVIQDGSLPTENIAIGSIDTI
                     VEAVKQENISAPAIIVIGEVVRKNPLAAYTLSLENHFLN"
     gene            complement(3355..5484)
                     /locus_tag="VFU15_13575"
     CDS             complement(3355..5484)
                     /locus_tag="VFU15_13575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008237073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HEU4718866.1"
                     /translation="MQSFRTETEIILPGGKTVIEKDILELEEKIRRFRNGTIDPEKFR
                     SLRLARGVYGQRQQGVQMVRIKIPYGKISPAQLRRIADLTEEFSTANIHFTTRQDVQL
                     HFVSLDRTPELWAKLEESDLTMREACGNTVRNVTASPEAGIDPEEPFDITPYADALFR
                     YFLRHPVGQDMGRKIKIAFSSSEKDSAFTFMHDVGFIPRVEIREGKIVRGFKVLIGGG
                     LGAQPFTARTAFEFLPAEEIIPFTETLLRVFDRYGERNNRHKARLKYLVNQLGADEVL
                     RFASLERISLAKQKIPIDGSAVFSPAKKEKTFTTFRVSESEEFKTWKDANTFPQKQDG
                     WYGVYVKITLGNISSVRARVLASLIEEFSEDDARVTPGQSILLRFVPANALPALFTAL
                     KENDFHATGAESLADITACPGTDTCNLGISNSTGVALELEKVVREEFPELVAEKNISI
                     RISGCMNSCGQHSLAQIGFHGSSVKAGTAVAPALQLLLGGGPTGSGNGRIAEKIGKFP
                     GRRAPAVLRTLLADFRTNANRDETFNDYFDRAGKNYFYNLVKPFFDVKTLAESELIDW
                     GQEERFVPAIGVGECAGVKIDLVSVLIAEAEDSLAAAEENLPETRYADAIYKTYNAFI
                     HSAKALLLSKEVHVNTQHGVLNDFDRHFVETGEFSFGKSFRETVLELNREKPSEEFAR
                     KYLGTANDFFRQIGQYRKTGQHGLLLN"
     gene            complement(5944..>6594)
                     /locus_tag="VFU15_13580"
     CDS             complement(5944..>6594)
                     /locus_tag="VFU15_13580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718867.1"
                     /translation="FFASCEGDVRFTTDQPGGQPALKTVPSELIGDYVNNDDSLYIRA
                     TEMTLVRPSRQTIPLKDSAKVGMTKTKDGRYIMRAGAQRYVENVSADSAVIVNRNADV
                     YHLGKDTLLKDFNGCYWLSMKSGENEWKIMQITLHKKKLGIAVPSLPKDERSKMNKRL
                     DEAKCSVDSAGVFSCVTPFTRSSDQSYFIVSATPDQLKNLDRRGLFRPVATFDKVK"
BASE COUNT         1391 a         1753 c         1799 g         1651 t
ORIGIN      
        1 atgttcgcct gcagcacttc cgaatggttg agcacgtcgt aaagaccttg tcccacgcaa
       61 ccgaagccga ataaagcgac ggaaatcttc tcgttattgt ctggcatctt atttcaattt
      121 tgaatgttga gttctgaatt ctgaatttga ttttgtctcg aacggttttc attaacagtg
      181 aactttaaac ccgaaacttt ccaactgtta tatatgcaac ccgcattccg ttttattccg
      241 gttcacccac cttccttccc gtccgcgtcc tttcaccgtg cagtgaatgc atcccactga
      301 attaaaacca tgcgcgacga gcgggtgtgg cggtaaatta ttttccgcaa tgtacttttt
      361 cgcttcctct tccgacaggt cgatgatggg atagaacttc agcagggttt ccttctgctc
      421 gaaaatagtc atctgtttcc ggtaattgtt ctgcgactgc atcagcccgg aaacccagac
      481 ttcatagccg gctttcagct tatcgagcgg ttccaccttg ttgatggagc agcagaggtc
      541 gggatctttg ctccacgtct ggtcggaatt cgtgaaggca tttttccatt cttccggctg
      601 cgcggtgcgg acgttaagcc cgaatctttc cgtgagcatt tccttgtacc ggagcgtttc
      661 cgcaaaatga taagtggtgt cgagaaaaat aatttcctgt gcaggattca cttcgtggaa
      721 catgtgcagc aggataacgg acgttgtacc gaacgaggaa gtgagcagca ctttttccga
      781 atcggaatac agcttttcga tccgctgcag cggcctcagc gaagcataca cgttatttaa
      841 ttcctcaaca cttttcacag cagcgccgaa attattgttt taagactcgt gatgatcacc
      901 agcagtccga caagcgcgag gccgttgcgc acggaaatgc gcgtggaaag ccagatggaa
      961 aacggggcgg ccaccattcc gccgatgatc agcccgagga tgatcgtcca gtgcgcgaaa
     1021 ccgatcatga agaagaacgt gacggagctg atgagtgcga cgaaaaattt tgcggcatgc
     1081 gccgacccga tggagtagcg caggtctcgt ccgccggcca gcagcgtcga cgttacgatc
     1141 ggtccccagc cgccgccgcc gatggaatcg agaaaaccgc cgaacgaagc aagcacgccc
     1201 acgcggcgga tcttcgacct tttctttttc ttctccatga tggctttgcg caaaattaag
     1261 acgccgagca caagagtgta cacggaaatc accggcttca cgtaatggac gtattctttc
     1321 agcagcgaga tcgtgcaggc gccaaggatc gcgccgatcg caccgggaac cagcagtgaa
     1381 cggaaaagct tgccgttgat gttgcggtat ttgaggtaca cgagcgaaga cgttcccgtg
     1441 agcatgatct ccgacgcgtg catgctggcg ctcgccaccg cgggcggaat cccgaacgaa
     1501 agaagtgaag cggtcgttgt cacgccgtac gccatgccca gcgctccgtc cacgagctgc
     1561 gcgcccatgc cgatcgcgat ccagataagg atgcggctgt cgatcgatga aagcgacccg
     1621 gtgatggagc tgccgatgcg ttcgaaggga acgtacgtga acagcaggtg cccgaggatc
     1681 atcagcgaga tcgccgaaaa agaatagaac agccacgagc gcagcttcag cccggtgaat
     1741 tttttcttcg catctttttc caccagcacg gccgtcgcac ggttcagcgc ttccactttc
     1801 tcacggaaat ttcctttcag gtgtttccga agttcgtgca ggtgcgcgat cgtcacctgc
     1861 gtttcgtcgg ggatggaatc gttcagcact tccttcatcc gcttcgagaa cgtcggtgat
     1921 tttccattgg tggaaacggc gatcttcagg tcgcctttct gcacgatgga accgaggtaa
     1981 aaatcacaga agcccggcgt gtccgcaacg ttcacgagca ggtttctccg gtgcgcttcg
     2041 atcacgagct cttccgccag cacaaaatca ttgaccgcaa taaaaacaat atccgcttca
     2101 tcgaaatcct gcggactcac ttcgcgttcg aaaatgatga cgctggaaaa ttctttcgca
     2161 agctccagga tctcatggcg tatttcgggc gcgatgagcg tgacattcgt ggccgggctg
     2221 ttggacagca ctgcgttcag cttctcgagc gcgacgttcc ctccgccgat gataagcagg
     2281 cggaggtctt cgagcttcat gaagaccggg aacagggtat ttcccctgtg cgtatgtgtc
     2341 agtgtgtcca gcgtacctgt ttttaagatc agcgcaaaag tgatgatttt cccggtaacg
     2401 tcacccgcgg gaaaccggtg ttgcttttca gttcaggaaa tggttttcaa gtgaaagtgt
     2461 gtacgccgcc agcgggtttt tccgcaccac ttccccgata acgatgatcg cgggtgcgga
     2521 aatattttcc tgctttaccg cttccacgat cgtgtcgatg gagccgatgg cgatgttttc
     2581 cgtcggaaga cttccgtcct ggatcacagc gacgggaaga tttccccgct cgtgttcctg
     2641 gtaaatgcgc acgatctttt ccaggttgcg caggcccatg agtatgacga ccgtcgcgcc
     2701 cgatcgtgca gcgagcttta tgtcttcagg cacttttcct tcgctgttgc gcccggtgat
     2761 cacccagaag ctttcgctgg ttccgcggtg cgtgacagga atgcgctgca gcgcgggaac
     2821 accggtggaa ctggaaatgc cgggcacgat atcggtttca atgttgaacg tttcggcgta
     2881 ctcgagctct tcggttccgc ggccgaacac gaacggatcg ccgcccttga ggcggacaac
     2941 atgcccgtgc gtgaacgcat agtcgacgat cagcgtgttg atctgttcct gcgtgaagga
     3001 atggttgccg ctgcgtttcc cgacgaaaac ttttttggcg tctgcgggcg cgtacttgag
     3061 aatttcagga ttgacgagcg catcgtacag gacgacgtcc gcttccccga gcgcattcac
     3121 gccttttatc gtgagcaggt caggatcgcc gggaccagcc ccgacgagcg ttactttcgg
     3181 ttgtatgttt tgtgttgaca tgatttcttg ttgttttaat agaaaatgtg gtttggtttc
     3241 tctgcgaaag ttctccgctt tcgcgtcttt acggttgacc agggtggagg gtcatgaaca
     3301 caggttcaac acgcctcgaa aaccgattcg agcagttgtt ttatcttttt catttcagtt
     3361 cagtaataat ccgtgttgtc cggttttcct gtattgaccg atctgccgga aaaaatcatt
     3421 cgctgtcccc aggtatttcc gtgcaaattc ttccgaaggt ttttccctgt tcagttccag
     3481 caccgtttcg cgaaagcttt ttccgaacga aaattctccg gtttccacga aatgccggtc
     3541 gaaatcattc agcacgccgt gctgtgtgtt cacgtgaact tccttcgaca gcagcaacgc
     3601 ttttgcagaa tggataaatg cgttgtacgt tttgtagatc gcatcggcgt aacgtgtttc
     3661 cggaagattc tcttctgccg ctgccaggga atcttccgct tccgcgataa gaacggaaac
     3721 gagatctatt ttcacgcctg cgcattcgcc cacgccgatc gcaggaacga acctttcttc
     3781 ctgtccccag tcgatgagct cgctttccgc cagcgttttc acatcgaaga acggtttgac
     3841 gaggttgtag aaatagtttt tccccgcgcg atcgaaataa tcgttaaacg tttcatcgcg
     3901 gtttgcgttc gttctgaaat ccgcgagcag cgtgcgcaat acggccggtg cgcgccggcc
     3961 cgggaacttg ccgatcttct cggcgatgcg tccgttgccg gaaccggtgg gtccgccgcc
     4021 aagcagcaat tgcagcgcgg gcgcaaccgc tgttcccgct ttcaccgagc ttccgtggaa
     4081 accgatctgt gcgagggaat gctgcccgca gctgttcatg cagccgctga tgcggatgga
     4141 aatgtttttt tctgccacga gctcggggaa ttcttcgcgc actacttttt ccagctccag
     4201 cgcaacaccg gtgctgttcg aaatgccgag gttgcacgta tcggttcccg ggcaggcggt
     4261 gatatcggcg agcgattctg cgccggtggc gtgaaaatca ttttctttca aagcggtgaa
     4321 cagcgctggc agtgcattcg ccggaacaaa acgcaaaagg atgctctggc cgggcgtgac
     4381 gcgcgcgtcg tcttccgaaa attcttcgat gagggaagcg aggacccgcg cacggacgga
     4441 tgaaatattt ccgagcgtga tcttgacgta aacgccgtac cagccgtcct gcttctgcgg
     4501 gaacgtgttc gcgtccttcc atgttttgaa ttcttcgctt tcgctgacgc ggaacgtcgt
     4561 gaatgttttt tcttttttgg ccggtgaaaa aacggcgctc ccgtcgatgg ggattttctg
     4621 cttcgcaagc gaaatacgtt cgagcgaagc aaagcgaagc acctcatccg cacccagctg
     4681 gttcacgagg tacttcagcc gtgccttgtg gcggttgttg cgttcaccgt aacggtcgaa
     4741 cacgcgcagg agcgtttccg tgaacgggat gatctcctcc gccgggagaa attcaaacgc
     4801 cgttctcgct gtgaacggtt gcgcgccgag tccgccgccg atcagcactt tgaagccccg
     4861 cacgatcttt ccttcgcgta tttccacgcg cgggatgaat cccacgtcgt gcatgaacgt
     4921 gaacgcgctg tctttttccg aagaggaaaa agcgatcttg atcttgcggc ccatgtcctg
     4981 cccgaccggg tgacggagga aataacggaa cagcgcatcc gcgtagggag tgatgtcgaa
     5041 cggctcttcc gggtcgatgc cggcttccgg tgaagcggtg acgttgcgca ccgtgttccc
     5101 gcacgcttcg cgcatggtga ggtcgctttc ttcgagcttc gcccataatt ccggtgtgcg
     5161 gtcgaggctg acgaaatgca gctgcacgtc ctgccgcgtg gtgaaatgga tgttggcggt
     5221 ggaaaattct tccgtgagat ccgcgatgcg gcgcagttgt gcgggcgaga tcttcccgta
     5281 gggaattttg atgcgcacca tctgtacgcc ttgctggcgt tgtccatata cgccgcgtgc
     5341 gagacgcagg ctgcggaatt tttccgggtc gatcgtcccg ttgcggaaac ggcggatctt
     5401 ttcttccagc tcgaggatgt ctttttcgat caccgttttt ccgccgggga ggatgatttc
     5461 tgtttcggtt ctgaagcttt gcatgacgct ttttttgttt tcactttttt gttttttccg
     5521 gaaccgggaa gacggaaaaa aataagccct ccacggtttt ccgggagggc ttcgctcaac
     5581 acaccaacaa tcaacatcac cgtgatacat agccttccct gctactgctc atgcagcagc
     5641 acatgcacat gtaacacatg caagtggaga ggaaggtttt agcggtgcgt ctcatgctga
     5701 gaactttgaa tttcgttgaa ataaaaaagg cttatcctgt ttccggaaaa gcctttgtat
     5761 aggtttatac tgatccgggt tattcgaatg cgcagcagca catcatggtc atgttgtgca
     5821 tcgtgttcat tgtatggatc agctgtgtgt tcattttgtt tgcgcgacaa atataaagcg
     5881 aaaaaaacga tcaagtcaag agcgcagaaa aaaaaccggg aattaccaat agagatccgg
     5941 gcgctacttc accttatcga acgtagccac gggcctgaac aatccccggc ggtcgaggtt
     6001 tttcagctgg tcgggagtgg ccgagacaat aaagtacgac tggtcggaag agcgtgtgaa
     6061 cggcgtgaca caggaaaaga cgccggccga atcgacagaa cattttgcct cgtcgaggcg
     6121 tttgttcatt ttgctgcgct cgtccttcgg cagggacgga acggctatgc ccagcttctt
     6181 tttatgcagc gtgatctgca tgatcttcca ttcgttctcg ccgcttttca tgctcagcca
     6241 gtaacagccg ttgaaatctt tcaggagcgt gtccttgccg agatgataaa cgtccgcgtt
     6301 gcggttgacg atcacggccg aatcggcgga aacattttcc acgtagcgct gcgcacccgc
     6361 gcgcatgatg tagcgcccgt ctttcgtttt tgtcattccc actttggcgg aatccttcag
     6421 cgggatagtc tggcgcgacg gccgcacgag cgtcatttcg gtggcgcgta tataaaggga
     6481 atcgtcgttg ttcacgtaat cgccgatgag ctcggaagga accgttttaa gcgcaggctg
     6541 cccgccgggc tggtcggtgg tgaaacgcac atcgccttcg caggaggcga aaaa
//
LOCUS       DASTSF010000468         3295 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_968829,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000468 DASTSF010000000
VERSION     DASTSF010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3295
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_968829"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..436)
                     /locus_tag="VFU15_13585"
     CDS             complement(<1..436)
                     /locus_tag="VFU15_13585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008614634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HEU4718868.1"
                     /translation="MEDSYLHKGLRRKLAGEIKAKGITDERILKALEKIPRHFFMDNA
                     FLRFAYEDKAFPIGAGQTISQPYTVAFQTQLLALVKGEKVLEIGTGSGYQTAVLCELG
                     VRVWTVERQKTLYDKTREFLPSLGYKPSFYYGDGYKGLKAFAP"
     gene            540..1541
                     /locus_tag="VFU15_13590"
     CDS             540..1541
                     /locus_tag="VFU15_13590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014201273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU4718869.1"
                     /translation="MLKIGVLGAGHLGKIHLRLIKEIANYELAGFFDPSESVSAQVAA
                     DFNTRAFPDADSLIEACDVIDIVTPTIHHFAAAEKAIRKSKHIFIEKPLTNTIEEGKM
                     LVRLASEANVKIQVGHVERFNPAFLAARSFIENPMFIVTHRLAQFNPRGPEVSVVLDL
                     MIHDIDIVLSLVKANIRRISASGVAVVSETPDIANARLEFDNGAVANLTASRISLKNM
                     RKSRFFRRDAYISVDFLEKSVNVVRMKNVTGEADPLALLIDLGEKGKKQIFFESPKTD
                     PVNSIKMELETFAQAIEGNTRPLVPIEDGFKALEVAHKIIDQLNLNANFAAAGAINA"
     gene            1568..2410
                     /locus_tag="VFU15_13595"
     CDS             1568..2410
                     /locus_tag="VFU15_13595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718870.1"
                     /translation="MKKSLSAFYFQLLTGGIFLFLFSGCKVIDPAEEIPCYIHIDKIT
                     LLNNEPSKITDAWVFVDNQLIGAFELPCTFPVLVKSGDHDILVRAGIKMNGSATTRAI
                     YPHYKGWESTVTLTQGQSTTIQPTAEFFSGVQFLWTEDFESPGNALVAESTSPANVWK
                     DNTDVYEGSNSGIITLNTDSAYFVGRTAQPYALSTTSDVYLEMNYKSDVTITVGVETP
                     DHQYRPWVDVAPSAVWNKIYINLNDVVIQQPIYTSYYMYIACSKPADVSECKVHLDDM
                     ILLQ"
     gene            2417..>3295
                     /locus_tag="VFU15_13600"
     CDS             2417..>3295
                     /locus_tag="VFU15_13600"
                     /inference="COORDINATES: protein motif:HMM:NF025104.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEU4718871.1"
                     /translation="MNKTWQVTKYVLSDALAASGAWILFYYYRKARIESVKFGVEVPV
                     IFDERFWQGLTGITLFWLSLYALTGTYRNIYRKSRLRELGQTLLYSFIGVLIIFFVLL
                     LDDTVIDYRSYYLLTFTLLITHFLFTASGRFVLSSFTNSRIRNRKLGFNTVLIGSNAN
                     ALKLFNDLESQKKSWGNKFVGFVHVDQKNGYSQELKAKLPHLGEVENLDHIISEEKAE
                     EVIIAIESSEHDRLNSIINTVDTGKVLIKVVPDMYDILSGQVKMSTIYGAPLIEINRE
                     IMPAWQESIKRIIDICA"
BASE COUNT          800 a          895 c          833 g          767 t
ORIGIN      
        1 aaggcgcgaa agccttcaat cctttatagc cgtcgccgta atagaaggac ggcttgtaac
       61 ccagtgaagg caggaactcc cgcgttttat catacagcgt cttctgccgc tcgacggtcc
      121 atacgcgcac gcccagctcg cacaagaccg ctgtctggta accggaaccg gtgcctattt
      181 ccaggacttt ttctcctttt acaagagcca acagctgcgt ctggaaagcg acagtgtacg
      241 gctgcgagat ggtttgcccg gcgccgatgg gaaaggcttt gtcttcgtaa gcaaaacgaa
      301 ggaatgcatt gtccataaaa aaatgccgcg ggatcttttc cagcgctttc aatatgcgct
      361 catcggtaat tccttttgct tttatttcac cggcaagttt gcgccgcaat cctttgtgca
      421 ggtaactgtc ctccattctc acgatccgtt atggtaacaa cacagtgttg aaacattgcg
      481 aagctaagat tcgtgcaaac gcggccgcac ggatacattt gtaaaaaatc aaaagggcaa
      541 tgttgaaaat cggcgttctc ggtgcggggc atcttggtaa aattcacctg cggctgatca
      601 aagaaatcgc gaattacgag ctggcgggtt ttttcgatcc ttcggaatcc gtttctgccc
      661 aggtggctgc agacttcaat acccgcgcct tccccgacgc cgattcactg atcgaagcgt
      721 gcgacgtgat cgacattgtc acacccacca ttcaccattt cgctgcagcg gaaaaagcga
      781 tccggaaatc gaagcacatt ttcatcgaaa agccgctcac gaatacgatc gaagaaggaa
      841 aaatgctcgt gcgcctcgcc tcggaggcga acgtgaagat ccaggtggga catgtcgaac
      901 gtttcaaccc ggctttcctg gctgcgcgct cgttcatcga aaacccgatg ttcattgtaa
      961 cgcaccgcct ggcgcagttc aacccgcgcg gaccggaggt gtccgtggtg ctcgacctga
     1021 tgatccacga catcgacatc gtgctcagcc tggtgaaggc caatatccgg cgcatcagcg
     1081 caagcggcgt ggccgtggtg agcgaaacgc ccgacatcgc gaatgcgcgg ctcgagttcg
     1141 acaacggtgc tgtggctaac ctcacggcca gccggatttc gctcaaaaac atgcggaaat
     1201 cgcgtttttt ccggcgcgac gcttacattt ctgtcgattt tctcgagaaa tccgtgaatg
     1261 tcgtgcggat gaaaaatgtg accggtgaag ccgacccgct ggcgttgctg atcgatctcg
     1321 gcgaaaaagg aaaaaaacag atcttttttg aatcaccgaa aaccgatccc gtcaattcca
     1381 taaagatgga gctggaaaca tttgcgcagg cgatcgaagg caatacgcgt ccgctcgttc
     1441 ccattgaaga tggtttcaaa gcccttgaag tggcgcataa aattattgac cagctcaacc
     1501 ttaacgctaa ttttgcggcc gcaggtgcaa taaacgcata agttttgcgt ttttaagcgc
     1561 ggtccgaatg aagaaatctt tatctgcttt ttatttccag ctgcttaccg ggggaatttt
     1621 cctgttcctt ttttccggtt gcaaggtcat tgaccccgcg gaagaaattc cctgctacat
     1681 ccacatcgat aagatcaccc tgctgaacaa cgagccgagc aagatcaccg atgcctgggt
     1741 tttcgtcgac aaccagctta tcggtgcgtt cgagcttccc tgcacgtttc ccgtgctggt
     1801 caaatccggc gatcacgaca ttctcgtccg cgcaggaata aaaatgaacg gttccgccac
     1861 aacgcgcgcc atttacccgc attacaaagg atgggaatcg accgtgacgc ttacgcaggg
     1921 acaatccaca acgatccagc ccaccgcgga gtttttcagc ggggtgcagt tcctgtggac
     1981 cgaagatttt gaaagcccgg ggaacgcgct cgttgcggaa tcaacttcac cggccaatgt
     2041 gtggaaagac aataccgacg tgtacgaagg ttcgaactcc ggcatcatca cgctgaatac
     2101 cgattccgct tatttcgtcg gaaggacggc gcagccctac gccctttcca ccacctctga
     2161 tgtttacctc gaaatgaatt acaaatcgga cgtcacgatc accgttggtg tggaaacgcc
     2221 cgatcatcaa taccgtccct gggtggatgt cgcgccgagc gcggtgtgga acaagatcta
     2281 catcaacctg aacgacgtcg tgatccagca gccgatctac acttcctact acatgtacat
     2341 cgcctgctcg aaaccggctg atgtttccga atgcaaggtg catctcgacg acatgatttt
     2401 attgcaatag gattttatga ataagacctg gcaggtgacc aaatatgtgc tcagcgatgc
     2461 gctggcagct tccggcgcct ggatcctgtt ctactattac cgcaaagcgc ggatcgaatc
     2521 ggtgaaattc ggtgtggaag ttcccgtgat cttcgatgaa cgtttctggc agggcctcac
     2581 cggcatcacg cttttctggc tttcacttta cgcgcttacc gggacctatc gcaacatcta
     2641 ccgcaaatcg cggctgcggg agctgggaca gacgctgctg tattcgttca tcggcgtgct
     2701 catcattttt ttcgtgctgc tgctcgatga tacggttatc gattaccgtt cctattacct
     2761 gctgacgttc acgctgctca tcacgcattt tcttttcacc gcgagcgggc gctttgtcct
     2821 ctcttccttc acgaacagcc gtatccgcaa ccgcaagctc gggttcaaca cggtgctgat
     2881 cggaagcaac gccaatgcgc tgaagctgtt caacgatctc gaaagccaga aaaaatcatg
     2941 gggaaataaa ttcgtcgggt tcgttcacgt cgatcagaag aacggctatt cgcaggaact
     3001 gaaagcgaag cttccgcacc tgggcgaagt agaaaacctg gatcacatca tcagcgagga
     3061 aaaggcggaa gaagtgatca tcgcgatcga atcatcggag cacgaccggc tgaattcgat
     3121 catcaatacg gtcgacacgg gaaaagtgct gatcaaagtc gttcccgaca tgtacgacat
     3181 tctttccggc caggtgaaga tgtcgacgat ctacggtgca ccgctgatcg agatcaaccg
     3241 cgagatcatg ccggcgtggc aggaatcgat caagcggatc atcgatatat gcgca
//
LOCUS       DASTSF010000469         4140 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1864708,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000469 DASTSF010000000
VERSION     DASTSF010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4140
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1864708"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..590)
                     /locus_tag="VFU15_13605"
     CDS             complement(<1..590)
                     /locus_tag="VFU15_13605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015809332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU4718872.1"
                     /translation="MGKIIAVANQKGGVGKTTTAINLAASFAVLEYRTLLVDADPQAN
                     STSGVGFDPRNVKTSIYECIIDGKDPRDIILQTSTPNLDLLPAHIDLVGAEIEMINMP
                     NREKMMRHALHKVKNDYDFIIVDCSPSLGLVTVNALSAADSVIIPVQCEYFALEGLGK
                     LLNTIKIVQSRLNTELVIEGILMTMYDGRLRLCNQVV"
     gene            complement(652..1374)
                     /locus_tag="VFU15_13610"
     CDS             complement(652..1374)
                     /locus_tag="VFU15_13610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEU4718873.1"
                     /translation="MKITYYGHSCFGVEIKGSHLLFDPFISHNPLASGIDIKRIKADF
                     ILVSHGHADHVGDTVAIAKHTGATVISVFEVCEWLGKQGVGKTHPMNVGGKWIFSFGK
                     VKCVNAVHSATLPDGSNGGCPMGFVIESEEGNFYFAGDTALHYDMKLIGEYRKIDFAF
                     LPIGDNFTMGVDNAIIAADFINCGNIVGMHYDTFGYIKIDHGDATGKFSRSGKSLTLM
                     KIGETMEKESLCGEQQKANSRQ"
     gene            1546..2067
                     /locus_tag="VFU15_13615"
     CDS             1546..2067
                     /locus_tag="VFU15_13615"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEU4718874.1"
                     /translation="MVTIISGTNRPGSTTAKVAAIYAKTLTGIGIESQVFSLEELPAD
                     FAFSYLQDRQNGDFRKLLEKYILPADRIVAVIPEYNGTFPGIFKLLLDAIHPRNLAHK
                     KMALVGVANGRGGNIRGIDQITNALHYMGVTVYHQVLPLSRLSEGTSASGAIDDANVL
                     NAIKKQAEGFVKF"
     gene            2166..>4140
                     /locus_tag="VFU15_13620"
     CDS             2166..>4140
                     /locus_tag="VFU15_13620"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEU4718875.1"
                     /translation="MKKILLSTVVFWCGFYVTAARAQSKTDSLQQVFAHHSSDTTGVQ
                     TLISLAGEYYMSDPAHAEATVRQSLALSEKLGYDAGRSASLGWLAYLLEQRGDADSAL
                     VLYNRVVLIDRKRANLKEEGQVLNNIAAIYKDKGRITEALDYYRQSIDLSRKANDPGS
                     LSSPLNNMGLIYQNQGQMKLALDYYGRSLAIEDSLHRKDGIATSLYNIATVYKDVGQY
                     DEALAYIRKSLEAERAIDDKYNMAYSVNYIGTIFEAQGKNREALAQYREALDVHRQID
                     DKEGEAYSLRNIAGIYEKLDSAEAAFSFYRQSLALFRGMENSWGEASVLCRLGTLFFD
                     RGETDSAAACGERSLELARKLGYPQDIRNAADLLRKVYRRQGKFEDAIGMYDLFVQMQ
                     DSVVSDQTKKAAIRSQFQLEYQASENALKQRIEREKLYRNSLIAGSVMLFLLALLLFS
                     RYRIKRAANVELEKKNRVISEERDRSDKLLLNILPEEVADELKENGSAKARNFNSVSV
                     MFTDFKDFTKISERLKPEELVSEIDYYFSAFDRIIEKYNIEKIKTIGDAYMCVCGLPA
                     AEPRHASLLVSAALEIRDFMESYSKEREAKGFIPFRVRIGIHSGQVVAGIVGVKKFAY
                     DIWGDTVNMASRMESNGEAGKVNISATTRSLAGD"
BASE COUNT          986 a         1052 c         1144 g          958 t
ORIGIN      
        1 accacctggt tgcagaggcg caggcggccg tcgtacatgg tcatgaggat gccttcgatc
       61 accagctcgg tattcaggcg gctctgcacg atcttgatcg tgttgagcag cttgccgagt
      121 ccttcgagcg caaagtattc acactgtacg ggaataatca cggaatcggc cgcgctgagc
      181 gcattgacgg tgacaagacc gagcgaagga gagcagtcga caatgatgaa atcgtaatcg
      241 ttcttcactt tgtgaagcgc gtgacgcatc atcttctcgc gattcggcat gttgatcatt
      301 tcgatctcgg cgccgacaag atcaatgtgg gcgggaagaa gatcgaggtt tggcgttgat
      361 gtctgcagga tgatatcgcg gggatcttta ccgtcgatga tgcattcata aatgctcgtt
      421 ttgacatttc gcggatcgaa accgacgccg gaagtggagt ttgcctgcgg gtcggcgtca
      481 acgaggagcg tccggtattc cagcaccgca aagctcgcgg cgagattgat cgcggtggtc
      541 gtttttccca cgcccccttt ctggttggct actgcaatta tttttcccat tctttgaatt
      601 caatattccc gttaccgggt taaaccgtag gctgttgact tgatttattt atcactgtct
      661 gctgttcgct ttttgctgct cgccgcacag ggattccttt tccatcgttt caccgatctt
      721 catcagtgtg agcgattttc ccgagcgcga aaatttaccg gtggcgtcac cgtgatcgat
      781 cttgatatac ccgaacgtgt cgtaatgcat gccgacgata ttgccgcaat tgatgaaatc
      841 agcagcgatg atggcattgt cgacccccat cgtgaaattg tcgccgatgg gaaggaaggc
      901 gaaatcgatc ttcctgtatt cgccgatcag cttcatgtcg taatgaaggg ccgtatcgcc
      961 ggcgaagtaa aaattaccct cttcgctttc gatcacgaat cccatgggac atccgccgtt
     1021 ggagccgtcg ggaagcgtcg ccgagtgcac tgcgttgacg cactttactt tgccgaagct
     1081 gaagatccat tttccgccca cgttcatcgg gtgcgttttc ccgactccct gtttgcccag
     1141 ccattcgcaa acttcgaaaa cggaaatgac cgtggcccct gtgtgcttcg caatggccac
     1201 ggtgtcaccg acatgatcgg catgtccgtg ggaaacaaga atgaaatcgg ctttgatcct
     1261 ttttatgtca ataccggaag caagcgggtt gtgcgaaata aaaggatcga aaagcaggtg
     1321 gctgcctttg atctccacgc caaagcacga atgcccgtaa taggttattt tcatactgat
     1381 gaatgcagga ttttaaaccg gtgcaaagcg aagatcgctg gttaaaattc cgttatttcg
     1441 tgcagtaaaa accgccagaa ataatttcgt ccgaaggtag tcaatttggg ggtagggaag
     1501 cgggatgaat ttttaacaaa gcgcggttga atcgttaaaa acccaatggt tacaatcatt
     1561 tcaggcacca accgccctgg cagcaccacg gcgaaagtag ctgcgatcta tgcaaagacg
     1621 ctgaccggca tcggcattga atcgcaggtc ttttcgctgg aagagctgcc tgccgatttt
     1681 gcgttcagct acctgcagga caggcagaac ggggatttca ggaagctgct ggaaaaatac
     1741 atactgcctg cagacaggat cgtcgccgtc atcccggaat acaacggcac atttcccggc
     1801 attttcaagc tgctgctcga tgcgatccat ccccgcaacc tcgcccacaa gaaaatggcg
     1861 cttgtaggcg tggcaaacgg ccgcggggga aatatccgcg gcattgacca gatcacgaat
     1921 gcattgcatt acatgggcgt gaccgtttac caccaggtgc tgcctttgtc aagactgagc
     1981 gagggcacgt ccgcctcggg cgctatcgat gatgcaaatg ttctgaatgc gataaaaaag
     2041 caggcggaag gtttcgtgaa attctgaaca ccgccgaccc gattccggtt tttgcaattt
     2101 cattttcgtc cggcaaaagg aaatttttat tttgacgtct ttatttccag gaagaaatag
     2161 acccggtgaa aaaaatcctt ctgtccacgg ttgttttttg gtgtggcttt tacgtcacgg
     2221 cagcaagggc gcagtcaaaa acagattctc tgcagcaggt cttcgcgcac cattcttccg
     2281 atacgaccgg tgttcaaacg ctcatttcgc tggccggtga atactacatg tccgatcctg
     2341 cccatgcgga agccacggtg cggcagtcgc tcgcactttc cgaaaagctc ggttacgatg
     2401 ccggccgttc cgcgtcgctc ggctggcttg cgtacctgct ggagcagcgg ggtgatgccg
     2461 attccgcgct cgtgctctac aaccgcgttg tcctcatcga ccgcaagaga gcaaacctga
     2521 aagaggaagg gcaggtgctc aacaacatcg cggcgattta caaagacaaa ggccggatca
     2581 cggaggcgct ggattattac cggcagagca tcgatttaag ccggaaagcg aacgacccgg
     2641 gttcgctctc gagtccgctg aacaacatgg ggcttattta ccagaaccag ggacagatga
     2701 agctggccct cgattactac ggacgcagcc tggccattga agattcgctt caccgcaaag
     2761 atggaattgc cacatcgctt tacaacatcg ccactgttta caaagacgta ggccagtacg
     2821 acgaagcgct cgcgtatatc cggaaaagtc tcgaggcaga acgcgccatt gatgacaaat
     2881 acaacatggc gtattccgtc aattacatcg gtacgatctt cgaagcgcag ggaaagaaca
     2941 gggaagcgct tgcgcagtat cgtgaagcgc tggacgtcca ccggcaaatc gacgacaagg
     3001 agggcgaggc gtattctctg cggaacatag cgggcattta cgagaagctg gacagcgcgg
     3061 aggccgcttt ttcattttac cgtcagagcc ttgccctgtt ccgcgggatg gaaaattcct
     3121 ggggagaagc atccgttctc tgccgtctcg gtacgctgtt cttcgatcgc ggtgagaccg
     3181 attctgctgc ggcctgcggg gagcgctcgc tggaactggc ccgcaagctc ggctatccgc
     3241 aggacatccg gaatgcagcg gaccttctcc ggaaagtcta ccgtcgccag gggaaattcg
     3301 aagacgcgat cggcatgtat gatctttttg tgcaaatgca ggacagcgtg gtcagcgatc
     3361 aaacgaaaaa agcggcgatc aggtcgcagt tccagctcga ataccaggct tcggaaaatg
     3421 cattgaagca gcggatcgag cgcgaaaagc tgtaccgtaa ttctcttatt gccggttccg
     3481 tcatgctttt cctgctggcg ctgctgcttt tcagccgtta ccgcatcaag agagcggcga
     3541 atgtggaact ggagaagaag aaccgggtga tctcggaaga gcgcgaccgt tcggacaagc
     3601 tgctgctgaa tattcttcct gaagaggtgg cggatgaact gaaggagaac ggcagcgcga
     3661 aagcaaggaa cttcaacagc gtgagcgtga tgttcaccga tttcaaggat ttcacgaaga
     3721 tcagcgagcg gcttaaaccg gaggagctgg tgagtgaaat tgattattac ttcagcgctt
     3781 tcgaccgcat catcgaaaaa tacaacatcg aaaagatcaa aacgattggc gatgcataca
     3841 tgtgcgtgtg cggtttgccg gcggcggagc cgcgtcacgc atcgctgctc gtcagtgccg
     3901 cgctggaaat acgcgatttc atggaatcct attcgaagga acgcgaagcg aaaggtttta
     3961 ttccattccg cgtgcgtatt ggcattcact cggggcaggt cgttgccggc attgtcggtg
     4021 tgaaaaaatt cgcttacgat atctggggcg acacggtgaa catggcttcc cgcatggaat
     4081 cgaacgggga ggcgggcaaa gtgaatatct ccgccactac gcgctcgctc gccggtgatg
//
LOCUS       DASTSF010000470         9355 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1870482,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000470 DASTSF010000000
VERSION     DASTSF010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 9355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9355
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1870482"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..554
                     /locus_tag="VFU15_13625"
     CDS             <1..554
                     /locus_tag="VFU15_13625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718876.1"
                     /translation="DHDHSRIEIYSDIADSYWILKKYDQSAINYKKRIDAAPDKATIS
                     NWNAYGMALFLNKDYAAADSAFTKITIMDPQNPAGWFRRGQANAYMDKDMKSDSARIF
                     YEKFYDLAITDKDKNKKDLVKACKYLAGYQYVQKNYACSKAYWQMILTLEPTDNDAKK
                     QLDDPQLKNAATVDVGTCKKGGK"
     gene            complement(876..3092)
                     /locus_tag="VFU15_13630"
     CDS             complement(876..3092)
                     /locus_tag="VFU15_13630"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEU4718877.1"
                     /translation="MKKTLLSFIVLSVVFLLDPQKILAQCPNDNTLAAGDLTPPGVTL
                     STTQNYAFGSYVLANVVAGANYTVSACSNSSFDTQLTVYDNSTGTLIAYNDDFCGLRS
                     QLSFTPTSCGQVRVLLDLYYCSASTSSADVTMTMNTPGTNQPSLVSATDQSACNGNTT
                     TIGISGNGSGGVPPYVYDWSPPLNLSSTTTPQTTATVTATQAYTLTLTDANGCMAKDT
                     VNVTVLPAPVVNLGADTAICGGPYTLNAGNPGSSYLWSTGAGTQTLVVTSSGNYAVNV
                     QNANGCSAGDNINVVVNPNPVFSLGPDTTSCSNPVTINGPGGYSGYLWTTGATTQSEA
                     FTFSATYGLTVTDANGCNGTDSIMVTLSPAPVVNLGPDTTQCGGTVTLNAGNPGSIYF
                     WSNNTSSQTTTVSSSGTYYVQVITPQGCSNSDTAVVTINNLPVVNLGADTAICGTSVT
                     LDAGNPGSAYTWSNSATTQAVTVGAGTYDVTVTDPSGCSDADTITVTTNPAPPVSAGP
                     DQAICPTQAATLTATGAIAYQWSTGAMTASTTVSPGVTTAYYVTGVDANGCSASDVVL
                     VSLMPASNAQFTDVVSGATATFTNQSTNAVTYSWNFGDATPLNNTANPSHTYTANGTY
                     TVTLTVSGPCGTDTYTQVVVITQVGIQDPDLASSLSLYPNPNDGNFTLSFDFTKQKDV
                     TIQVLDVTGRIVYSEEDHNISSYNKQLGLAGAGSGIYFVRIITDEGVTTQKIVIQR"
     gene            3183..5255
                     /locus_tag="VFU15_13635"
     CDS             3183..5255
                     /locus_tag="VFU15_13635"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU4718878.1"
                     /translation="MAKKVVKNKKDGAGTTSRKPAPRGFFRRNSFSIVLFLLTFVVFG
                     NGIFNEYALDDEFYTNSTNGGNKVTAKGFKGLHEVFTTRTFYNNDGSGYSYRPVALAS
                     FAIEIGLFGESPHMSHFINVLLYGLTVVILFRLMRRWFSTQGDWFSFFIALLFLVHPL
                     HTEVVDNIKCRDELLALLFGFLALLLVWKHMETGKKHYLLLYPLCVFAGMLSKHTIIP
                     VIALFPVAMWFFSNTDWKKIVLYMIPALLAMMANGMIERFGLPATSRTLQAFENPIPH
                     DSALLFRFATAAYVSIRYLYLHFVPYPLVYYYGFNYVPVVGISNPLVILSVIVYAALG
                     ILALRGLKKKTILSFGILFYLVNIAAYSNLLRPAPGIMAERYTYASSLGFCIVVIFLV
                     FKWTKLAPENFRWKAPAYSRARFILVGIAVLFAVRSIGRNEDWEDKETLYGGDMETLH
                     ESAKANMLYGALLSSRAMGLHLDANQKQAMGNDQQAKQEEAESRRMFIESRIYYRQAT
                     NIADYYHTAWSNLGTTYFFVDQPDSAIPYFRRAVKIRPDYTEGLFNLGMAYDKTGKLD
                     SAIYFFSSCIKNDSTYMQGYEQWARVKMQEENDPQGALALLHTAARKKPDSEVPWNNI
                     ATIYLQQHDTASAAGAMEMAAQINPSNIQRLANLARYFQMHQNIMKYNQYQSMYEEEM
                     RKQKPNQR"
     gene            complement(5246..6715)
                     /locus_tag="VFU15_13640"
     CDS             complement(5246..6715)
                     /locus_tag="VFU15_13640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718879.1"
                     /translation="MKILQHIIGTMNKEEVRHLKLFMGRTNSGDDRKDVELFDYIRQH
                     HEDYDESKIQHRLYGPRDKNALYRLKNRLLEDIGKSLALQYLNENDFNEVVHILVLSR
                     FFLSRNQGSVAFHYLQKAEKKATHAESPELMDIVYSEFVRFSQETLEVNPTEYIRKRR
                     SNREQLNRVQEIDDILAEVIFRLRTTQNFSGQDYRIIENLRRKVNEFSKQKSARTSTQ
                     LRFKIYHSLSRIFLQKQNFQALEKYLVQTLDEFSKENLFNRNNHDTRLQMLTYLANAQ
                     FKNGKSAESLATAARLKTAMNEFGGQLHDKYLFFYYNILVINYADTDRSRAIEILMEA
                     KDNPVIKKLPFYLVFIFLNLTVLNFDNGNYRQAIRNLVKLTMEPGFVNLDYVLRMKIA
                     VAELIIRYELGDFDVLERRLDQVKKEFADLLKKKEGKKQAQLIAIISMMVGSQSVKRD
                     KVLQKKIQRFAGMKTRDDSWENDVINYKSWLAPKMAGQR"
     gene            6843..7517
                     /locus_tag="VFU15_13645"
     CDS             6843..7517
                     /locus_tag="VFU15_13645"
                     /inference="COORDINATES: protein motif:HMM:NF039201.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEU4718880.1"
                     /translation="MLIFSSERIDAQTGIGFGAPGLIGMPDTVYSGYSTFIGAYLKNF
                     DTSFVYSDSIRIDGYVDTLSGPHIPFSLPASYLNGVNPGDSLFFPFWLTFKDTYMGGN
                     LRIGGNVIVVWPATFQSGFSTADSVTANIFLIDTLAGTGPEHLVPDDVKCFPVPASGP
                     LYISCVNPNLRPKSISVHDAGGRVIYVNDKPSSPIDTDSWAPGVYFLEIEFDNGGLRV
                     YKIMKN"
     gene            complement(7496..7813)
                     /locus_tag="VFU15_13650"
     CDS             complement(7496..7813)
                     /locus_tag="VFU15_13650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718881.1"
                     /translation="MKKDPAYGGIFFCLPVFQIVNAFLRAPYHNARIEKPWQIYRIAY
                     DNGSSLRQGNDARYRGRKDSVKRRNENRRNCRAIFPGKKDQREHSKHPEQGTFAVQFF
                     MIL"
     gene            complement(7830..>9355)
                     /locus_tag="VFU15_13655"
     CDS             complement(7830..>9355)
                     /locus_tag="VFU15_13655"
                     /inference="COORDINATES: protein motif:HMM:NF024974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HEU4718882.1"
                     /translation="TTYTVTATNGFGCTAEDSVAVTVTSAPPLTVSNDTAMCAGGAAT
                     LSASGSVNYTWQPGNLTGSTINVSPATTTTYVVTGNNNNCMSTDTIVVTISPPPSIFA
                     GPDFSICSGNQATLTTVSNATAYSWQPSSGIVGSNTTQNVVVQPTTNTSYTVTVTGPG
                     GCISSDTLNITVNPTPTVTATASPSAICIGQATTLTGSGASTYSWIPAVGVQNPASGN
                     TSANPTNTTTYQLVGTNAFGCMDTTSVTVTINPLPEVVVTPNATNCGDSTGSVTMNGV
                     VAGTGPFTYQIGSQTYNNLPITNLPAGTYTMITTDANGCVSSQTFGIPYVINSTVSAS
                     ASPDFGVYPLNVNFAASGTNGINNYVWSLGDSTQFANYTYGAPGTYTVIVAAWNDDVA
                     CTVYDTVVVTVIEQALIKMPNVFTPNNDGTNDWFAADVSGVSDITVEIFDRWGAQIYD
                     GSVNGISGSQQTVPLWDGKSKGGATAHDGVYYYVVTATGYDTKQYPMTGFVHLITTK"
BASE COUNT         2121 a         2413 c         2413 g         2408 t
ORIGIN      
        1 ttgaccatga ccattcccgt atcgagatct attccgatat cgcggattcc tactggatcc
       61 tgaaaaaata tgatcagtcc gcgatcaatt acaagaaacg gattgatgct gcgcctgata
      121 aagcgacaat ttccaactgg aatgcctacg gaatggcttt gttcctgaat aaagattacg
      181 ccgctgcaga ctctgctttt acgaagatca ccattatgga tccgcagaat cccgcgggct
      241 ggttcaggcg cggacaggca aacgcttaca tggacaagga catgaaaagc gacagtgcgc
      301 gtatttttta tgagaagttc tacgatcttg ccatcaccga taaagacaag aacaaaaagg
      361 atctcgtcaa agcctgtaag tatctcgccg ggtatcagta tgtccagaaa aattatgcct
      421 gctcaaaagc ttactggcaa atgatcctga cgctggaacc cacggataac gacgccaaga
      481 aacagctcga tgatccgcag ctgaaaaatg cagcaacggt ggatgtcggc acctgtaaaa
      541 aaggcggaaa ataattcgcc ttgcccttct gtttcaaaac cgcttccgtt cgtgaagcgg
      601 ttttttattt cgccacaggg tgttgtttcc tgtttccgtc tcccggtttt ttcattgaaa
      661 atgctccccg gacgcacgtt ctcctgtccc tcgaccgctt agagagctac gattatttcc
      721 cgccagcctg cagatgtatt ttgagcaccc tgcgtttttc cacgtggccg ctgcagccga
      781 ctttttcccc ggcgcagata acgcggggcc gttgactcct gcaaaagaaa agtcccggca
      841 taaccgggac ttcctcaatg acctgtttgc ttttctcagc gctggataac gatcttctgc
      901 gtcgtcacgc cttcatcggt aatgatccga acgaaataaa ttccagagcc ggcaccggca
      961 agtcccagct gtttattata cgatgagata ttgtgatctt cttcgctgta cacaatcctt
     1021 cccgtaacat ccagcacctg gatcgtaacg tctttctgct tcgtgaaatc gaacgacaga
     1081 gtgaagtttc catcgttcgg gttcggataa agcgaaagcg aagaagcgag atccgggtcc
     1141 tggataccga cctgggtgat caccacaacc tgggtatatg tatccgttcc gcaaggtccg
     1201 gaaacagtaa gcgttacggt atatgttccg ttggcggtat acgtgtgcga cgggtttgcc
     1261 gtgttattca gcggggtcgc gtctccgaaa ttccagctgt aggtaaccgc atttgtagat
     1321 tgattcgtga atgtggcggt agcgccggac acgacatccg tgaattgtgc gttggaggcc
     1381 ggcatgaggg aaacgaggac gacatcggat gcagagcagc cattggcgtc gacgccggtt
     1441 acatagtagg ctgttgtaac gcccgggctg accgttgtgg acgctgtcat tgctccggta
     1501 ctccactgat aagcgattgc ccctgttgcg gtaagcgttg cagcctgcgt cgggcaaatg
     1561 gcctggtcgg ggccggcaga aaccggcggg gccgggttgg ttgtgacggt aattgtgtcc
     1621 gcatccgaac agccggaagg atctgtaacg gtaacgtcat acgttccggc acccaccgtt
     1681 accgcctgtg ttgtggcgct gttgctccac gtgtatgcag agccggggtt cccggcatca
     1741 agcgttactg aagttccgca gatggccgtg tctgcaccga gattgacaac cggcaggttg
     1801 tttattgtca caaccgccgt gtccgagttg gaacaaccct gcggggtgat tacctgaacg
     1861 taatacgttc ctgaagaaga cactgttgtc gtctgcgacg acgtattgtt ggaccagaaa
     1921 taaatggaac ccgggttgcc ggcgttcagt gtcacggttc cgccacattg cgtagtgtcc
     1981 gggccaaggt tgaccaccgg tgcaggactc agcgtaacca tgatgctgtc tgttccatta
     2041 catccgtttg catctgttac tgtcaggcca tacgttgcgc tgaaagtaaa tgcctcggat
     2101 tgcgtggtgg ctccggtcgt ccagagataa ccggaatatc cgcccggtcc gttaatggtg
     2161 accgggttgc tgcatgaggt tgtatccggg cccagggaaa atacaggatt cggattgaca
     2221 acgacgttga tattatcgcc ggccgaacag ccattcgcat tctggacgtt cacagcataa
     2281 ttaccggatg atgtcaccac cagggtctgc gttccggcgc ccgtactcca caggtaagag
     2341 cttccgggat taccggcatt cagcgtataa ggtccgccgc aaatcgccgt gtctgcaccg
     2401 agattgacaa ccggcgccgg aagaacagta acgttcaccg tgtctttcgc catacagcca
     2461 tttgcatccg tgagcgtgag cgtatacgcc tgtgtggccg taactgttgc ggttgtctgt
     2521 ggtgtagtgg tggaggaaag attcagcggg gggctccagt cataaacgta cggaggaacg
     2581 ccgccgcttc cgttcccgga tatgccgatc gttgtggtgt tgccgttgca cgctgactgg
     2641 tccgtcgcgg acaccagcga aggctggttg gttccgggcg tattcatcgt catcgtcaca
     2701 tcagcggaag aagtggaggc agaacaatag taaagatcca gcagcactct tacctgtccg
     2761 cacgaagtcg gcgtaaatga aagctgcgag cgaagtccgc agaagtcgtc gttatacgcg
     2821 atcaacgttc ccgttgagtt atcatacacc gtcagctgcg tatcgaacga agaattgctg
     2881 caggcggaaa ccgtatagtt cgcaccggct accacgtttg ccaggacata agagccaaaa
     2941 gcataattct gggtggtgga aagcgttaca ccgggcgggg tgagatctcc cgcagccagg
     3001 gtgttgtcat tcgggcattg cgccagaatt ttctgaggat ccaaaaggaa aacaacagaa
     3061 agaacaataa aagagagtaa cgtttttttc atgcttgccg gttaacgtta gacgaataac
     3121 aaaagtacag aaaaagcgat aattccaagg ctttatcatt tactttgcgg taaatcccgg
     3181 caatggctaa aaaggtcgta aaaaacaaaa aggacggggc tggcacgaca agccgcaaac
     3241 ccgcgcccag gggatttttc cgccgtaact cgttttccat tgtacttttc ctgctgacgt
     3301 tcgtcgtttt cgggaatgga atattcaatg aatatgcgct ggacgatgaa ttttatacca
     3361 acagcaccaa cggcggcaac aaggtaaccg ccaaaggttt caaggggctt cacgaggttt
     3421 ttacaacccg gacattttat aacaacgacg gcagcggcta ttcctaccgg ccggtagcgc
     3481 ttgcttcatt tgccattgag atcggcctgt tcggggaaag tccgcacatg agccatttta
     3541 tcaatgtcct gctttacggc ctgacggtcg ttatcctttt ccggctgatg cggcgatggt
     3601 tctccacgca gggtgactgg ttttcgtttt tcatcgcact gcttttcctt gttcacccgc
     3661 ttcacaccga agtggtggac aacatcaagt gccgggatga gctgctggcg cttcttttcg
     3721 gatttctcgc cctgttgctg gtctggaagc acatggaaac ggggaaaaaa cactatctcc
     3781 tgctgtaccc gctttgcgtt ttcgccggga tgctttcaaa gcacaccatc atcccggtaa
     3841 ttgcgctgtt ccccgtcgcg atgtggtttt tttcgaatac cgactggaaa aagatcgtgc
     3901 tgtacatgat tcctgcgttg ctggcaatga tggccaacgg catgatcgag cgattcggtc
     3961 ttcccgccac gtcgcgtacg ctccaggctt ttgaaaatcc aattccccat gattccgccc
     4021 tgcttttccg tttcgcaact gcggcttatg tgagcatccg ttacctgtac ctgcattttg
     4081 ttccttaccc gctcgtttat tactacgggt tcaactatgt tcccgtagtt ggcatttcga
     4141 atccgctcgt cattctctct gtcattgtat acgccgccct cggcattctt gccttgcgcg
     4201 gactgaagaa aaaaacgatc ctttccttcg gtattctttt ttacctggta aacattgccg
     4261 cttattccaa cctgctgagg ccggctcccg ggattatggc cgaacgctat acctacgcct
     4321 cttctctcgg gttctgcatt gtcgtgattt tcctggtctt caaatggaca aagctcgccc
     4381 cggaaaattt ccggtggaaa gcgccggcat atagccgtgc acggtttatt cttgtcggga
     4441 tcgcagttct ttttgccgtg cgttccattg gccgaaatga ggactgggag gacaaggaaa
     4501 cgctttatgg cggcgatatg gaaacgctgc atgagtcggc gaaagcgaac atgctgtacg
     4561 gcgcgctgct ttcatcccgt gcgatgggcc ttcacctcga tgccaaccag aaacaggcca
     4621 tgggcaacga tcagcaggcg aaacaggagg aagcggaatc ccggcggatg ttcatcgagt
     4681 cgcgcatcta ttaccggcag gcgacgaata ttgcggatta ttatcacaca gcctggagca
     4741 acctcggaac aacttatttt tttgttgacc agcccgattc ggctattccg tatttccgcc
     4801 gcgccgtcaa gatccgaccg gattataccg aagggctttt caacctgggc atggcctatg
     4861 acaaaacagg aaaattagat tccgcgatct atttcttttc ttcctgcatt aaaaatgatt
     4921 cgacctacat gcagggatac gagcaatggg cgcgtgtgaa aatgcaggaa gaaaacgacc
     4981 cgcagggcgc gcttgcgctg ctgcacacag ccgcgcggaa aaagccggat tccgaagttc
     5041 cctggaataa cattgcaaca atatacctgc agcagcacga tacggcaagc gccgccggcg
     5101 caatggaaat ggctgcacag atcaatccat caaacataca gcgcctggcc aatcttgccc
     5161 gctatttcca gatgcaccag aacatcatga agtacaacca gtaccagagc atgtacgagg
     5221 aggagatgag gaagcagaaa cccaatcagc gctgacccgc catcttgggt gccagccagg
     5281 atttgtagtt gatcacatca ttttcccacg aatcatcgcg cgttttcatt cccgcaaacc
     5341 gctggatctt tttctgcagc accttgtccc ttttcaccga ttgtgatccc accatcatgg
     5401 atatgatcgc aataagctgc gcctgctttt tcccttcttt tttcttcagc aggtcggcaa
     5461 attctttttt aacctggtcg agtcgccgct ccagcacatc aaaatcgccg agctcgtaac
     5521 ggatgatcag ctccgccacg gcgatcttca tgcgaagcac ataatcgaga ttgacaaagc
     5581 cgggttccat cgtcagcttg acaagattcc ggatcgcctg ccggtaattc ccgttgtcga
     5641 aattcagaac ggtgaggttg agaaaaatga acacgagata aaacggaagt tttttgatca
     5701 cgggattatc tttcgcttcc atcaggatct cgatcgcgcg gctgcggtcg gtatcggcgt
     5761 aattgatgac gagaatattg taatagaaaa aaagatattt gtcgtgcagc tgcccgccga
     5821 actcgttcat ggctgttttg agccgtgccg ccgtagccag cgattccgcc gatttgccgt
     5881 tcttgaactg cgcgttcgcg aggtacgtga gcatctgcag gcgcgtatcg tgattattgc
     5941 ggttgaaaag gttctctttc gaaaattcgt ccagcgtctg cacgaggtat ttttccagcg
     6001 cctggaaatt ctgcttctgt aaaaagatgc ggctgagcga atgatatatc ttgaaccgca
     6061 gctgcgtgct cgtccttgcc gatttctgct ttgaaaactc gttgaccttc cggcgaaggt
     6121 tttcaatgat gcggtaatcc tggcctgaaa aattctgtgt cgtgcgcagg cgaaagatca
     6181 cttccgcaag aatatcgtcg atctcctgca cgcggttcag ctgttcgcgg ttgctcctgc
     6241 gcttacggat gtattccgtc gggttcacct ccagcgtttc ctgtgaaaac cgcacgaact
     6301 cggaatagac gatgtccatc agctcggggc tttccgcatg cgtggctttt ttttccgcct
     6361 tctgcaggta atgaaacgca acggaaccct ggttccggga aagaaaaaac cgggaaagaa
     6421 caagaatgtg aacgacctcg ttgaaatcgt tttcgttcag gtactggagc gcgaggcttt
     6481 tgccgatgtc ttcaagaagg cggtttttca ggcggtacag cgcattcttg tcgcgcggac
     6541 cgtacagccg gtgctgtatc tttgattcgt cgtaatcctc gtggtgctgg cgtatgtaat
     6601 cgaaaagctc gacatctttc cggtcgtcac cgctgttcgt tcttcccatg aaaagcttga
     6661 ggtgtctcac ctcttccttg ttcatcgttc cgataatatg ctggaggatt ttcatgcttt
     6721 ttgaacgcgt aagattattt caaatttaga tgtttttttg agttgaacgg ggtatttttg
     6781 ttaggaatca aaaggccatg ttccggaacg cccgatttat ttcgcgaatc gcagtcctgt
     6841 ttttgctgat cttttcctcg gaacggatcg atgcacaaac aggaatcggc tttggtgcgc
     6901 cggggcttat cggcatgccc gatacggttt attccggcta ttcgacattc atcggcgcgt
     6961 acctgaaaaa cttcgacacc tcatttgttt acagcgacag catacggatc gatggatatg
     7021 tggatacgct gagcggaccg cacatcccgt tctctcttcc ggcgtcttat ctcaatggag
     7081 taaatccggg agacagtctt ttctttcctt tctggcttac gttcaaggac acgtacatgg
     7141 gaggaaatct ccgtatcggg ggaaatgtga tcgtcgtctg gcccgccaca ttccagagcg
     7201 ggttttcaac tgccgattcc gtcacggcga atattttcct catcgacacg ctcgcaggga
     7261 caggaccgga acatcttgtg cccgatgatg tcaaatgttt tcccgttccc gcaagcggac
     7321 cgctttacat ttcctgcgtc aaccccaatc tccggccgaa atcaatttcc gttcacgatg
     7381 ccggcggacg cgtgatctac gtgaatgaca aacccagctc gccgatcgac acggattcct
     7441 gggccccggg tgtttatttt ctcgagatcg aattcgataa cggcggcctc cgcgtttata
     7501 agatcatgaa aaactgaacg gcaaacgtcc cctgctccgg atgttttgag tgttcacgtt
     7561 gatctttctt cccaggaaaa atcgcacggc agtttcttct gttttcgttc cgtcttttta
     7621 cggaatcttt tcttccgcga tatcgcgcgt catttccctg ccgcaaagag cttccgttat
     7681 catacgcgat gcggtaaatc tgccatggtt tttcgatgcg cgcattgtga tacggcgccc
     7741 gcagaaatgc attgacaatt tggaaaacgg ggaggcaaaa aaaaatcccg ccataagcgg
     7801 gatctttctt cacctgttat attctctgtt tattttgttg taatgaggtg tacaaaaccc
     7861 gtcatcgggt actgcttggt atcgtaaccg gttgccgtca ccacgtaata ataaacacca
     7921 tcgtgagccg tcgcgccgcc ttttgatttt ccatcccaca gcggtaccgt ttgttgcgag
     7981 ccggaaattc cgtttacgga tccgtcatat atctgcgcgc cccagcgatc gaaaatttcc
     8041 actgtaatgt cggaaacacc gcttacatca gccgcaaacc agtcgttggt tccgtcgttg
     8101 ttcggtgtaa atacgttcgg cattttgatc agggcctgtt cgataaccgt taccacgacc
     8161 gtatcataca cagtgcaggc gacatcgtcg ttccacgccg ctacaataac cgtgtatgtt
     8221 ccgggcgctc cgtacgtata atttgcgaac tgggtggaat cgccaagaga ccacacataa
     8281 ttgttgatcc cgttcgttcc gctggcagca aaattcacgt tcagcggata aaccccgaag
     8341 tcgggagatg ccgatgcgga caccgtagaa ttaataacat acggaattcc gaatgtctga
     8401 ctcgaaacac agccattggc gtccgttgtg atcattgtat acgttcccgc aggaagattg
     8461 gtgatcggca ggttgttata ggtctggctg ccgatctggt acgtaaatgg tccggttccc
     8521 gccacaacac cgttcattgt cacactgcct gtcgagtctc cgcaattggt tgcgttaggc
     8581 gtgacaacaa cttccggaag cgggtttatc gttacggtta ccgaagtggt gtccatacat
     8641 ccgaatgcgt tcgttcccac aagctgatag gttgtggtgt tggtcgggtt tgctgacgta
     8701 tttccagaag ccggattttg aacacctacc gcaggtatcc acgaataggt cgatgctccg
     8761 gaaccggtaa gtgttgttgc ctgcccgatg caaattgcgg acgggcttgc cgttgcggta
     8821 accgttggcg tggggttaac cgtaatgttc agcgtgtctg atgaaataca tccaccgggt
     8881 cccgttacgg taaccgtata cgacgtattc gttgtcggct gaaccaccac attctgtgtt
     8941 gtattgcttc cgacgatgcc ggaggagggc tgccagctat acgctgtcgc gttagatacg
     9001 gttgtcagcg ttgcctggtt tccgctgcaa atggaaaaat cgggaccggc aaaaattgac
     9061 ggcggcggag aaatggtcac cacgatcgta tcggtgctca tgcaattgtt gttgttcccg
     9121 gtcacaacat acgtcgttgt tgtcgccgga gaaacgttta tcgttgatcc cgtaaggttt
     9181 cccggttgcc aggtgtagtt gacggaaccg ctcgcgctca gcgtcgcagc accgcctgca
     9241 cacatggctg tgtcattcga aacggtaagc ggcggagcag aagtaaccgt aacggctacg
     9301 gaatcttctg ctgtacaacc gaacccgttc gtggcagtga ctgtgtatgt tgttg
//
LOCUS       DASTSF010000471         8358 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_935478,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000471 DASTSF010000000
VERSION     DASTSF010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 8358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8358
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_935478"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..784)
                     /locus_tag="VFU15_13660"
     CDS             complement(<1..784)
                     /locus_tag="VFU15_13660"
                     /inference="COORDINATES: protein motif:HMM:NF028237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HEU4718883.1"
                     /translation="MRDFFHLDFSHIGIVSLFFLPALINAGIFFYSWFFAPESKVNSS
                     FSVLVLCLAGWQTAEGFVHVSATSGAADKWDTISGLMLTLSVAYGVLFSMRLCGLYDK
                     IKRSIVRPAIFLPAIVIIFLQVEMPEIRTIHSSGSWNWVATPAGTPLSFGIYAWLCAG
                     GLFMAGIFWTFAFRRSEDETRRKLFLLLGVGFAVPVLAGIVAEVLFPFFSGTDDVPIT
                     TSAVTAFSIASFIAMRRYRLLEYSPVHQWDTIVESMNEGMLIV"
     gene            complement(941..2839)
                     /locus_tag="VFU15_13665"
     CDS             complement(941..2839)
                     /locus_tag="VFU15_13665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEU4718884.1"
                     /translation="MSNGIPVSLSLKPTLEAENIGPDEAPDKKRILAISFYALLVSVV
                     VCIAARFLVFLIEFFTGLFYYGRLSGAHEGPAGNTLGAWAILVPVAGGLIVGLMAYYG
                     SKAIRGHGIPEAMEQILTNQSRIRPSIIYLKPLSAAISIGTGGPFGAEGPIIATGGAF
                     GSAVGQLLKISDQERKVLLTAGAAAGMSAIFGSPLAAIFLAIELLLFEFSPRSIIPVS
                     LSCIVGAAGHHLLFGNEPVFAMHDAGIISSPALAGYCAIGVAMGLLSAGVTKAVYFIE
                     DLFEKIPFHWAWWPAIGGVAVGIAGYFDPRTLGVGYNNITDVLAGNMTLRLLLSLCII
                     KFVSWAIALGSGTSGGTLAPLLTIGGAAGALLGLAMRSWLPHCEVSVSLSALMGMSAM
                     FAGSSRALITSIVFALETTSCTTALLPLIGACTASYFVSFFMMKNTIMTEKIARRGVR
                     TPQSYEPDVLEKTNAADAMKWIEEEVLFSSENTIGEIMQWQKTHADFDRPYFIVTTPA
                     GEFLGIVETAGLFQKHDPRTEIGHLLSPGGAFADAGMSLRTVAGIMAREKRYVMPVMS
                     AQEGRHVTGIISAQEIIAVYDDERKSHQRKEKHLSFRRGTLKILLRGQKMISLIRTKN
                     ADRKPQDH"
     gene            complement(2987..4600)
                     /locus_tag="VFU15_13670"
     CDS             complement(2987..4600)
                     /locus_tag="VFU15_13670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718885.1"
                     /translation="MTAQAPSWAFATQYGSAYSQSAYRIAVDKNDNYYVAGQFAYDLW
                     IGPVHLQGHAPSGSWNIFLAKFGPGGNVLWAREFGGFCTDLITGMSIDTLGGIFLCGS
                     FCDTINIDNVHLLAADISGSNNSYVIKFDGNGNALWGKDIVGKSPCRVECVAADGAGG
                     VFAGGYFSDTLITGAQVLHTTGVDDAFISRIGASGNTLWAQRDGGAYYEAIQYLCTDN
                     AHHVYAGGKYTNSSVFGSYALNNAPGQDDFFVASYDFSGGNANWATDGSIGGPDNINY
                     LGGIAYDNIGGIVLSGGFTGTLSFTGSATGISPNYDDNFLVRMDTLGIPSWQTHGGNG
                     GADDGKSIACDHFGNIYQVADFFGNVVFDTLQVTNPNMSSFATTAIVRYSPGGHAQWA
                     LLPACTSLYGFYDVAVRDGNPVVCGEFYNDITLGGTPMNAVGAPDIVITEAAFPMGIG
                     ETPRPAFSTDCFPDPATDLIRLRAPSAGHFRVSIYDDEGNLAFCCECDAAEKNDILSI
                     DSSLLPPGAYVGQLTGNVCTAPFRFMIAR"
     gene            complement(4691..6079)
                     /locus_tag="VFU15_13675"
     CDS             complement(4691..6079)
                     /locus_tag="VFU15_13675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine 2,3-aminomutase"
                     /protein_id="HEU4718886.1"
                     /translation="METMETKMKAAPGTVYTAYSLNNFRKIPQAERLSEEQKSDIEVV
                     GSVLPFKVNSYVVNELIDWDNIPQDPIFRLTFPQKEMLSPEHFAQMKKALLSGDGRNH
                     IKSVANSIRMQLNPHPAGQATHNVPELDGVKLTGIQHKYRETVLFFPSQGQTCHAYCT
                     FCFRWPQFVGMDEMKFAMRESELLAKYVRLHPEVTDVLFTGGDPMIMRTKIFEGYVND
                     LLNDPAPNLQTIRIGTKALGYWPYRFTTDNDADDLLRLFERIVKSGINLAVMAHFNHP
                     VELSTREVQKAIARIRATGAQIRTQSPLLRHINDSADVWAEMWRKQVNLNCIPYYMFL
                     PRDTGAQEYFAVPIVESWEIFRQAYQQVSGICRTVRGPSMSCTPGKVQVLGVTKIPGV
                     IGMKKVIVLRFLQGRDPDWAARPFFAEYDPSALWMDELKPALGSGKFFFEDELEQMFH
                     TKISGNEADSFE"
     gene            6632..7366
                     /locus_tag="VFU15_13680"
     CDS             6632..7366
                     /locus_tag="VFU15_13680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU4718887.1"
                     /translation="MQTTGNTILITGGTSGLGLAFAEEFLKRGNKVIICGRRKDRLGA
                     LQKTHPGLAAIECDIADEKQREMLAEKVIREFPGVNMLMNNAGVQLAVDLTSPVKAER
                     IRAEIETNLVAPIHLATLFVKHLASQSNPAILTVSSGLAFCPIAFMPIYCATKAAIHS
                     YCLSLRYQMRNTPVKIFEIIPPSVDTELGHERRTDKTQTHGGMPVAAFLSEAMEAIEN
                     DLLEAPVADAKGLRSKGEAMFPVMNR"
     gene            complement(7509..8222)
                     /locus_tag="VFU15_13685"
     CDS             complement(7509..8222)
                     /locus_tag="VFU15_13685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718888.1"
                     /translation="MKKLALFVFLSVVSLVPVSAQSYLEIGYRYGIMHPGNINRGVYT
                     YNYTRPWLSQKMDFFKVMKGWEFSYMRRTDGAGLEFSMGRISGINNAFGIEPTTGQMA
                     YRKIKFGTGGFSAGLRYFTMQTAHFESGPSLDFDFNFVVCETWYAHDPSFANAQDDVV
                     FSKFKMANTIAWNFSFYAAPWLGINIRPYYQQPWGKVNVEGMANYLQGVDGDVEREKT
                     TNFGISGTLVLAFRRGKLF"
BASE COUNT         2047 a         2308 c         2182 g         1821 t
ORIGIN      
        1 tcacgatcag cataccttcg ttcatggatt ccacgatagt atcccattgg tgaaccgggg
       61 aatattcgag cagccggtaa cgccgcatgg caatgaagga ggcaatggaa aacgcagtga
      121 cggcagaggt ggtgatggga acatcgtcgg tgccggaaaa aaaggggaac agcacttcag
      181 ccactatccc ggcgagcaca ggaacggcaa aaccaacacc gagaagcagg aaaagtttcc
      241 gcctggtctc gtcttcgctt ctccggaatg cgaacgtcca gaagatccct gccatgaata
      301 gtccgcctgc gcacagccac gcataaatgc cgaaagaaag cggcgtgcct gccggcgttg
      361 ccacccagtt ccatgaaccg gaagaatgaa ttgtgcgtat ttcgggcatt tccacctgca
      421 gaaaaatgat cacgatggcc ggcagaaaga tcgccgggcg gacaatgctc ctttttatct
      481 tatcataaag tccgcaaagc cgcatggaaa acaggacacc ataagctacc gaaagcgtca
      541 gcatcaggcc cgagatcgta tcccatttat ccgcggcacc cgatgtggcg gaaacatgca
      601 caaacccttc ggctgtctgc cagccggcga gacaaagcac aaggacggag aaggagctgt
      661 tgactttgct ttcgggcgca aagaaccagg aataaaaaaa tattccggcg ttgatcagcg
      721 cgggcaggaa gaacaagctc actatcccga tatggctgaa atcgaggtga aaaaaatcac
      781 gcatggggga aattttagaa caccgaaatg tatatcatcc gggcatcccc gtgcggacaa
      841 aaaagcgaat tggattacag gtgtaagatt ttcgcgaatt atgcagcgta cgaacaggaa
      901 acagccacgg ccgaacccgc cggctctgaa aacaggaaga tcagtggtcc tgcggtttcc
      961 ggtctgcgtt ttttgtcctg atgagggaga tcatcttctg cccgcgaagc aggatcttca
     1021 gcgttcccct gcggaaagac aaatgttttt ctttccgttg atggcttttc cgttcatcat
     1081 catacacggc gatgatctcc tgcgcggaaa ttattcccgt tacatgccgt ccttcctgtg
     1141 cagacataac cggcattaca taacgttttt ccctggccat aatgccggca accgtgcgca
     1201 ggctcatgcc cgcatcggcg aaggcgcccc cgggagaaag gagatgccct atttctgtgc
     1261 gcggatcgtg cttctgaaac aacccggcgg tttccacgat acccaggaat tcaccggcgg
     1321 gtgttgtaac gatgaaatag ggacggtcga agtccgcgtg cgttttctgc cactgcatta
     1381 tttcaccgat cgtattttcg ctgctgaaca gcacttcttc ctcgatccat ttcatggcat
     1441 cagccgcatt cgtcttttca agaacgtccg gttcatagga ttgtggcgtc cgcactcccc
     1501 ttctcgcgat cttttccgtc ataatggtat tcttcatcat gaaaaaagaa acaaagtatg
     1561 acgccgtgca cgcgccgatc agcgggagaa gagccgttgt gcaggaagta gtttcaaggg
     1621 caaatacaat ggacgtgatg agggcgcgtg atgatccggc aaacatagcc gacatcccca
     1681 tgagtgccga aagcgagacc gaaacttcgc aatgcggcag ccagctgcgc atcgcaagac
     1741 cgagcagcgc acccgccgcg ccaccgatcg taagcagcgg ggccagtgtt ccgcccgagg
     1801 ttccacttcc aagcgcaatg gcccaggaga caaattttat gatgcagagc gaaagcagga
     1861 gccgcagcgt catgttccct gcaagtacat cggtgatgtt gttataacct acgcccagcg
     1921 tccgcggatc aaaataaccg gcgatgccga cagccactcc gcctatggcg ggccaccatg
     1981 cccagtgaaa aggtattttt tcgaaaaggt cttcgatgaa gtaaaccgct tttgtcactc
     2041 cggccgagag aagtcccatg gcaactccga tggcgcaata tcccgcaagc gcagggcttg
     2101 agattattcc tgcgtcatgc atggcgaata caggttcgtt cccgaaaagc agatgatgtc
     2161 ctgccgcacc aactatgcag gaaagtgaaa caggaatgat cgaacgcggg gaaaattcga
     2221 agagcagcaa ttctattgcg agaaatatgg cggccagcgg gcttccgaaa atggcggaca
     2281 ttcccgccgc ggcgccggcc gtgagaagca ctttacgctc ctggtcggag atcttcagca
     2341 gctgccctac cgccgatccg aacgcgccgc cggttgcaat gatgggacct tccgcaccga
     2401 acgggccgcc cgtgcctatc gagatggcag cggaaagcgg tttcaggtaa atgatggaag
     2461 gacgtatccg gctctggttt gtaagtatct gctccatcgc ttcgggtatg ccgtgcccgc
     2521 gtatggcctt cgaaccgtaa tacgccatca gtcccacgat cagcccgccg gcgacaggaa
     2581 caaggatagc ccacgcgcct aacgtattac ccgccggacc ttcgtgtgcg cccgaaagcc
     2641 tgccgtaata aaaaagcccc gtgaaaaatt caatgagaaa aacgaggaaa cgcgcagcga
     2701 tacatacgac cacacttacc agcaatgcat aaaacgaaat ggccagtatc cgttttttat
     2761 caggcgcctc gtccggcccg atgttctccg cttcaagcgt cggtttaaga gatagtgaaa
     2821 ccggtatgcc gttgctcatt ttcataaatg tcctgcacct cgaccatgca taaagaaaga
     2881 aaatacagga tgaacgggac gctgccacct gcgcggtgca aaattaggtc tgcaaaaaac
     2941 aaataagttg agatttatca atttcccgcc gccgcatctt tccctgctac ctcgcgatca
     3001 taaaacggaa cggcgccgtg cacacgttac ccgtcagttg tcccacatag gcgccgggcg
     3061 ggagcaatga actatcgatc gaaagaatat catttttttc cgcggcgtca cattcgcagc
     3121 agaacgcaag atttccttcg tcgtcgtaaa tgctgacccg gaaatgcccg gccgaaggag
     3181 cgcgcagccg gataagatcc gtcgcaggat cgggaaaaca atccgttgaa aaagccggac
     3241 gcggcgtctc gccaattccc atcgggaagg cggcttccgt gatgacgata tccggtgcgc
     3301 ccaccgcatt catcggggtg ccaccgagcg taatatcatt gtaaaattct ccgcacacga
     3361 ccgggtttcc gtcgcgcacc gccacgtcgt agaatccgta aagcgacgtg cacgcaggca
     3421 gcagcgccca ttgcgcatgg ccgccgggtg aatagcgtac aattgccgtt gtagcgaatg
     3481 agctcatgtt cgggttggtc acctggagcg tgtcgaaaac aacgttgccg aaaaaatccg
     3541 ccacctggta aatattaccg aagtgatcgc aggcaatact ttttccgtca tctgctccgc
     3601 cgttgccacc gtgcgtttgc caggagggaa ttccgagcgt gtccatgcgg acgagaaaat
     3661 tatcatcgta attgggactg atcccggtag cagaacctgt aaagctgagt gtcccggtga
     3721 atcctcccga aagcacgatg cctccgatgt tgtcgtaagc tattccgccg agataattga
     3781 tgttgtcggg tccgccaatg cttccgtccg ttgcccagtt cgcatttcct ccgctgaaat
     3841 cataggacgc gacgaaaaag tcatcctgcc cgggtgcatt attcaacgca tagcttccga
     3901 aaacagagct gttcgtatac ttcccgcctg cataaacatg atgcgcattg tcggtgcaca
     3961 ggtactggat ggcttcataa taagcgcctc cgtcgcgctg ggcccagagc gtatttcctg
     4021 atgcgccgat acgcgagatg aacgcatcgt cgaccccggt tgtatgcagc acctgtgcac
     4081 cggtgatgag cgtgtcggaa aaatagccgc cggcaaaaac accgcccgct ccgtcggcag
     4141 cgacacattc cacgcggcag ggactttttc ccacgatgtc ttttccccat aacgcatttc
     4201 cgttcccgtc gaattttatc acgtatgaat tgttcgaccc ggaaatgtcc gccgccagga
     4261 gatgaacatt gtcaatgttg atcgtatcgc agaaacttcc gcagagaaaa attccgccga
     4321 gtgtatcaat gctcatgccg gtgatcaggt ctgtgcagaa tccgccgaat tcccgcgccc
     4381 agagaacatt tccgcccggg ccgaactttg cgaggaaaat attccacgaa cccgaaggcg
     4441 catgcccctg caggtgtaca ggccctatcc acaggtcata ggcgaactgt cctgccacgt
     4501 aataattatc gtttttgtca acggcgatcc tgtaggcaga ctggctgtac gccgaaccat
     4561 actgcgtggc gaatgcccag gaaggagcct gcgccgtcac tgaagcgatg cggcaaaaaa
     4621 acaggaagaa aattaataca gcgccacgca tcggggttcg ttatacgcgc aatttcgaaa
     4681 aataagcccg tcattcaaag ctgtccgctt cgttcccgct gatctttgta tggaacatct
     4741 gctccagctc atcctcgaag aaaaatttcc cggaaccgag cgcaggcttc agctcatcca
     4801 tccacaaggc ggaaggatca tattcggcga agaaaggacg cgcagcccag tccgggtcac
     4861 ggccctggag gaagcgcaac acgatcactt ttttcatccc gatcacaccg ggaattttcg
     4921 tcacgccgag cacctgcact ttcccgggcg tgcacgacat gctcggaccg cgcaccgtgc
     4981 ggcagatacc gctcacctgc tggtacgcct ggcggaagat ctcccatgat tccacgatgg
     5041 gcacagcgaa atattcctgc gcacctgtgt cgcgcggcag gaacatgtag tacggaatgc
     5101 agttcaggtt cacctgcttt ctccacatct ccgcccacac atccgcggaa tcgttgatgt
     5161 ggcgaagcag cggcgactgc gtgcggatct gcgcgccggt ggcgcgtatg cgcgcgatag
     5221 ctttctgcac ttcacgggtg gacagctcaa ccgggtgatt gaaatgcgcc atgaccgcga
     5281 ggttgatgcc ggacttcacg atgcgttcga acaggcgcag caggtcatcc gcgtcattgt
     5341 cggtggtgaa acggtacggc cagtagccga gcgctttcgt cccgatgcgg atcgtctgca
     5401 ggttcggcgc aggatcattg agcaaatcat tgacataacc ttcaaaaatt ttcgtgcgca
     5461 tgatcatcgg gtcgccgccg gtgaacagca catccgtgac ttccgggtga aggcgcacgt
     5521 atttcgcgag cagttccgat tcacgcatgg cgaatttcat ttcatccatt cccacgaact
     5581 gcggccagcg gaaacagaac gtgcagtaag cgtggcaggt ttgtccctgc gacgggaaga
     5641 acaaaaccgt ctcgcggtat ttgtgctgga tcccggtgag cttcacgccg tccagctccg
     5701 ggacattgtg cgtggcctga ccggcaggat gcggattgag ctgcatgcgg atgctgttcg
     5761 cgaccgattt gatgtggttg cggccgtcgc ctgacaaaag cgcttttttc atctgcgcaa
     5821 aatgctccgg cgaaagcatt tctttttgcg ggaacgtgag ccggaagatc gggtcctgcg
     5881 gaatgttgtc ccagtcgatc agctcgttca ccacgtagct gttgaccttg aaagggagca
     5941 cgcttccgac gacttcgata tccgacttct gctcttcgct caatctttcc gcctgcggaa
     6001 ttttcctgaa attattcagc gagtacgctg tgtaaactgt gccgggggcg gccttcattt
     6061 tcgtttccat cgtttccata cgttcacggt ttaaggtttg acaaaaaaag aattcagacg
     6121 gcaacactgc attccgggaa aatccgcccg gcgataaaat gaaaccgcgc gtggttcagc
     6181 aaccacgttc atacaccggg gtgttttgca gcagtaaaat ttacagaccg gtattttaca
     6241 aatgggaaca gggcagaaaa caccggcatt ctcccgaaca atccgtcatc acagcgcgaa
     6301 aataatgacg atatttttca actgcgttga tatttctcaa tgcaaactcc ggattccaaa
     6361 cggcacacaa aaaaacgatt cacgtcctac gttaaagttt cgggttccgg gtttaaagtt
     6421 tgagaaacat ccaggcctga atagcgttga ccgttgttct ctgtgtttcc tctgtgaagc
     6481 agcctccgtg ctcctctgtg ttaaaaagaa attttcagtt tcgggcttga attgtgtcga
     6541 agcctgaata acgttaacca tttaaaccgg ggcgcttccc gcaaagaaca ttctgctgta
     6601 ctttcgtacg taacatttca aacagaagat catgcagacc acaggaaaca ccatcctcat
     6661 cacaggcgga acatcgggac tcgggctcgc gttcgcggaa gaatttctga aacgcgggaa
     6721 caaagtgatc atctgcggaa gaagaaaaga caggctcggc gcattgcaga aaacacatcc
     6781 gggtcttgcc gcgatcgaat gcgacatcgc cgatgaaaag cagcgtgaga tgctcgcgga
     6841 aaaagtgatt cgggaatttc ccggcgtcaa catgctgatg aacaacgcgg gtgtgcagct
     6901 ggccgtcgac cttacctcac ccgtgaaagc agaacgcata cgcgccgaga tcgaaaccaa
     6961 tctcgttgcg cccattcacc tcgcgacatt atttgtaaag catctcgctt cgcaaagcaa
     7021 cccggccatc cttaccgttt cctcgggact cgctttctgc cccatcgcgt tcatgcccat
     7081 ttactgcgcg accaaagcgg cgatccattc gtactgcctt tcgctgcgtt accagatgcg
     7141 gaacacgccg gtgaaaattt tcgagatcat tcctccttcc gtggatacgg agctggggca
     7201 tgagcgcaga accgacaaaa cgcagacgca cggcggaatg ccggtggccg cttttctttc
     7261 agaagcgatg gaagcgatcg aaaacgatct tctcgaagcg ccggttgccg atgcgaaggg
     7321 attgcgttca aaaggagaag cgatgttccc ggtgatgaac cgctgagtaa attgacccgg
     7381 gtgtctctta aattcctggc cgtttttttt cctgcgatcc ggcctttcag cgatgacatt
     7441 ttggggacgg cgcagtgatc cgctcttgcc ggcgaagccc aaagcgatgc gcaattattt
     7501 tcgcgtgatc agaacagctt gccacggcga aaggccagca caagcgttcc ggaaatgccg
     7561 aagttcgtcg ttttctcgcg ctccacgtcg ccgtcgacgc cctgcaggta attcgccatt
     7621 ccttccacgt tcacttttcc ccagggctgc tggtagtacg ggcggatgtt gatgcccagc
     7681 cacggcgccg cgtaaaatga aaaattccag gcgatcgtgt tcgccatctt gaacttgctg
     7741 aaaacaacat cgtcctgcgc gttggcaaac gacgggtcgt gagcgtacca ggtttcgcaa
     7801 accacgaaat tgaaatcgaa atcgagcgaa ggtccgcttt cgaagtgagc cgtctgcatg
     7861 gtgaaatacc gcagcccggc actgaagccg ccggtgccga attttatttt gcggtaagcc
     7921 atctggcccg tcgtcggctc tatgccgaac gcattgttga taccgctgat gcgccccatg
     7981 ctgaattcca gtccggcacc gtccgtccgg cgcatgtagg agaattccca tcctttcatc
     8041 actttgaaaa aatccatttt ctgcgacagc cagggacgcg tgtaattgta cgtgtaaaca
     8101 ccgcggttga tgttcccggg atgcatgatg ccgtaccggt agccgatctc gagatagctc
     8161 tgcgcggaaa caggaacaag cgacacgacc gaaagaaaaa cgaagagagc cagttttttc
     8221 atcatcatca gtctttaacg cgtttaagca cgatgtgctc ttttatattt ccgctgaagg
     8281 aattgtaata catgttgttc atcggtcctg aataatcgag cgtcatttta ttccacccgc
     8341 ggtccgaaat atcatacg
//
LOCUS       DASTSF010000472         6200 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1017768,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000472 DASTSF010000000
VERSION     DASTSF010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 6200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6200
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1017768"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1137)
                     /locus_tag="VFU15_13690"
     CDS             complement(<1..1137)
                     /locus_tag="VFU15_13690"
                     /inference="COORDINATES: protein motif:HMM:NF040455.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HEU4718889.1"
                     /translation="MKRFLFFLVPACILVFGGCGGKPENPAGETQVVADDPEKINKLI
                     ADYLHDHFAKADTSKRFRISADSITAYPEVIKFYSSLGDKPIWSDKGKLNASGDSMWA
                     VVKDAESYGLIPGDYHYSVIDSLLKNSYDSVKEQFNVNRLAQTDMMLTDAFFTFAVHV
                     SVGRIDNDSSTARSWKIHRLDTDLVSVLASTLKANTFRKTFAALEPQRMEYQSIKKYM
                     NDYRRKYAGITWAHLPDRKEDSTGFFAGVKERLIEYGDYDTASTEKDSLKLSNALKKF
                     QKRFFLEQDGKIGRNTILTLDMTPQDWTRQLAMNMERWRWEPEKFEKRHMIVNLPVFK
                     MTVWEADTIVMQSKIVCGAVKTQTPELDSKMNQIILYPFWNVPYS"
     gene            1417..1698
                     /locus_tag="VFU15_13695"
     CDS             1417..1698
                     /locus_tag="VFU15_13695"
                     /inference="COORDINATES: protein motif:HMM:NF014534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HEU4718890.1"
                     /translation="MEITIQSLHFTARPSLDEFVREKVTALEKFNGKILSADVYLKLD
                     KAVEGNKVCELKIALPGEKLVAKKQDSSFEKATVSAVEAMKQQLESRRN"
     gene            complement(1716..2318)
                     /locus_tag="VFU15_13700"
     CDS             complement(1716..2318)
                     /locus_tag="VFU15_13700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718891.1"
                     /translation="MKRIFLLAMLSAAFGSCCIYRPSIAQSDRIDTHRPPFPIVDCVP
                     CHDLRNRSENFSWMEVMGPSGSFAAGMEAGRNFSYLRLYGAAGISGWAWRGPNASFLA
                     NAGIAIGNPNFFVRPDIGAGFSEWWLKDPRGKTVQLHPALKTSVEKGGYYPAWNVYGG
                     LRFAFPFSSVNLSLRLYRVSTFGNTSASAWYPGIACGFRF"
     gene            complement(2373..3317)
                     /locus_tag="VFU15_13705"
     CDS             complement(2373..3317)
                     /locus_tag="VFU15_13705"
                     /inference="COORDINATES: protein motif:HMM:NF024599.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEU4718892.1"
                     /translation="MKKSLLTVLAAGAFALTGFAQQYIPTAGFETWGSSFGEDQQPQN
                     WISYNVFTAPVFDPGGTNPTSVTPAPTPDNYQGAYSAKIVTVDLVTNQSPTTIPNRAG
                     LLMIGSISFTSPYLHPGYLFGSRPATLTYYSKYTPVNGDTAYCLVLLTHWNSSNSTRD
                     TVATGLDWTNTSTTSYAQRTVALVYNPNDANVTPDTCTIVISASSNYTPQAGSTLYVD
                     ALSFSGFVGINEQDHAPNPVNVYPNPSNTVTNFDVTSEDAYEVVVYDMTGREVKREQI
                     MNKKGTIDSSTMDNGMYSYHIIAKSGDVLSSGQFAVSR"
     gene            complement(3431..5278)
                     /locus_tag="VFU15_13710"
     CDS             complement(3431..5278)
                     /locus_tag="VFU15_13710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014202420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU4718893.1"
                     /translation="MKNFFGVLKYVRGYWRYGWLNIFFNILSVIFGLFTLGMIKPFLD
                     LITVKSDDVYQKILAEGKPSLGASFNSVMKYLNYEFSDLIMHEGKMKALVMICIGVGV
                     IFFFKALFRYLGMFFLAPIRNGVVKDLRNSIYEKTLRLPLSYYSNERKGDIMSRITTD
                     VQEVEWSVMSSLEMIFRDPVNILIILGFLVYISPALTLVIFILLPVAAFLISTVSKSL
                     KRSSNRAKEELGTLFSIMEETLGGLRIIKGFNAEKSMNKKFGKVNEHYTKQMISTYRK
                     VDLASPMSEFISSAIMMVLIYFGGRLVVGAGATMEGSTFVLYIITFSQVIAPAKTFTG
                     AYSNIQKGIVSIDRINKILHAEETIRDKSDAKKVSGFEKEIEYRDLSFAYLRGDQGYA
                     LKNINLKIPRGKTIALVGQSGSGKTTLADLLPRFYEPTSGQVLLDGTNTGDLRIHDLR
                     ALMGIVSQESILFNDTVLANIAFGVESATKEDVIAAAKVANAHDFIMEMPDGYETNIG
                     DRGSKMSGGQRQRIAIARAVLKNPPILILDEATSALDTESERLVQDALNKLMQNRTSL
                     IIAHRLSTIQHADEIIVLQKGEIIERGNHAELLARNGVYRKLYDMQVFV"
     gene            5394..5906
                     /locus_tag="VFU15_13715"
     CDS             5394..5906
                     /locus_tag="VFU15_13715"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEU4718894.1"
                     /translation="MKSKTAKESLAIATHHVLPNDTNNLNNMFGGRLLEWMDIIAAVS
                     AHRHCRRVVVTATVNHVAFHEGIPYGSIVTLQAKVSRAFNTSMEVFIDVFVENQVSGE
                     QLKTNEAIYTFVAVDQNGSPLPVPELVPETEEEKSRFDGALRRRQLALILAGKMKPDE
                     ATELKALFVK"
BASE COUNT         1380 a         1566 c         1772 g         1482 t
ORIGIN      
        1 gctgtaagga acattccaga acgggtagag gatgatctgg ttcatcttgc tgtcgagctc
       61 cggtgtctgc gttttgactg cgccgcacac gatcttcgac tgcatcacga tggtgtctgc
      121 ttcccacacc gtcattttga aaacgggcag gttgacgatc atgtggcgct tttcgaattt
      181 ttccggctcc catctccagc gttccatatt catggcgagc tgtcgcgtcc agtcctgcgg
      241 cgtcatgtca agcgtgagta tcgtgttgcg cccgatcttt ccgtcctgct cgaggaaaaa
      301 acgtttctgg aatttcttga gcgcattgga aagcttcagc gaatcctttt cggttgacgc
      361 ggtatcgtaa tcgccgtatt ctatgagccg ttctttcaca ccggcgaaaa atcccgtgct
      421 gtcctctttg cggtcgggaa gatgcgccca cgtgatgccg gcgtatttcc ggcggtaatc
      481 gttcatgtat tttttgatcg actggtattc catccgctgc ggctcgagcg cggcgaatgt
      541 cttgcggaat gtgttcgcct tcagcgtcga tgccaggacg gaaacaaggt ccgtgtcgag
      601 ccggtgtatt ttccatgacc tcgcggtgga gctgtcgttg tcgatgcgcc cgacggaaac
      661 gtggacggca aacgtgaaga acgcgtcggt gagcatcatg tccgtttgcg cgaggcggtt
      721 gacgttgaat tgttccttga cggaatcgta ggaatttttc agcaggctgt cgatgacgga
      781 ataatgataa tcgccgggaa tgagcccgta gctttcggcg tctttcacca ccgcccacat
      841 ggaatcgccg gatgcgttca gctttccctt gtccgaccag atgggtttgt cgccgaggga
      901 agaatagaat ttaatgactt cgggatacgc tgttatggaa tcggcggaaa tccggaaacg
      961 tttgctggta tctgcttttg caaaatgatc gtggagataa tcggcgatca gcttgttgat
     1021 cttttccgga tcgtcggcaa cgacttgggt ttcgccagcc ggattttcag gtttgccgcc
     1081 gcatccgcca aaaacaagga tgcaggctgg taccaggaaa aaaagaaaac gtttcattgc
     1141 gggtttgtcc gtcggacttc ttaggagcgg tatcaaaatt aggtaaaagc gtattggtgc
     1201 cgaaagggat tttcaacacc cggtgacttt tccgcataaa tgtggtgaac acggggatac
     1261 ccgtccggtt atgccgtacc ttgtgcattg ccggagcagc ggtcgggtca ttgatgcaaa
     1321 tcattgttcc tcctgcgctg ctgcagggcg ggaagggcga agagtaaaga acttagcacc
     1381 cgttcacaga aaagtaacct gagataatac aaggatatgg agatcacgat tcaatcattg
     1441 cattttacag cccggccttc actggatgaa tttgtcaggg agaaagtaac ggccctcgaa
     1501 aagttcaacg gaaaaatatt gtcggccgat gtgtacctta aactggacaa agcagtggaa
     1561 gggaataagg tatgcgaatt gaaaatcgcg ctgcccggcg aaaagctggt ggcgaaaaag
     1621 caggacagct catttgagaa agcaacagtg tctgcggtcg aagcgatgaa gcagcagctg
     1681 gaaagcaggc ggaactgatc gggagaagga acttctcaga agcgaaaccc gcacgcaatc
     1741 ccgggatacc acgcggacgc tgatgtattt ccgaaagtag aaacgcggta gagccgcagg
     1801 cttaaattca cggacgagaa cgggaacgca aaacgcaacc cgccatatac attccatgcg
     1861 gggtagtaac cgcccttctc gacggaggtt ttcagcgcgg gatgaagctg caccgttttt
     1921 ccccgtggat ctttcagcca ccattctgaa aatcccgcac cgatatccgg gcgcacaaag
     1981 aaattcgggt tgccgatcgc gatgcccgca tttgcaagga aggaggcgtt cggtccgcgc
     2041 caggcccacc cggaaattcc ggcggccccg tacaaacgca ggtagctgaa atttcttccc
     2101 gcttccattc ccgccgcaaa acttccggaa ggtcccatga cttccatcca cgaaaaattt
     2161 tcgctccggt tgcgcaggtc gtggcaggga acacaatcca caatgggaaa cggcggacga
     2221 tgtgtatcga tgcgatcgct ttgcgcgatc gatggccggt aaatgcagca ggagccgaaa
     2281 gctgcggaaa gcatcgcgag cagaaaaatt cttttcataa aaaaacccgt tatacaaaag
     2341 cataacgggt cggatgaaaa aggttacagc gcttaccggc tcacagcgaa ctgcccgctg
     2401 gaaagaacgt ctccgctttt cgcgatgatg tggtaggaat acattccgtt atccatcgtg
     2461 gaagaatcga tcgttccttt tttgttcatg atctgttcgc gtttcacttc gcgtcccgtc
     2521 atgtcgtaca ccaccacttc atacgcgtct tccgaagtga cgtcgaaatt cgtgaccgtg
     2581 ttcgaagggt tcgggtaaac gttcaccggg ttcggtgcgt ggtcctgctc attgatgccg
     2641 acgaagcccg agaaagaaag tgcgtccacg taaagcgtgc ttcctgcctg cggcgtgtag
     2701 ttcgagctgg cgctgatcac gatcgtgcag gtgtcaggcg ttacgtttgc gtcattcggg
     2761 ttgtacacga gtgcaaccgt gcgctgtgca tacgaagttg ttgaggtatt ggtccagtcg
     2821 agaccggtag ccaccgtatc gcgcgtggaa tttgaactgt tccagtgggt gaggagaacg
     2881 aggcagtagg ctgtatctcc gttcaccggc gtgtatttcg aataatacgt aagcgttgca
     2941 ggacggctgc cgaacagata gccgggatgc aggtacggag aagtgaaaga gatggaaccg
     3001 atcatcagca ggcccgcgcg gttggggatc gtggtcgggc tctggttggt gacgaggtcg
     3061 acggttacga tcttggcgga gtatgcgccc tggtaattgt ctggtgtcgg cgccggggtg
     3121 acggacgtcg ggttggttcc gcccggatcg aagacaggtg ctgtgaacac gttgtaggaa
     3181 atccagttct ggggctgctg gtcttccccg aagctggaac cccacgtttc aaaaccggca
     3241 gtgggaatgt actgctgtgc gaacccggtc agcgcgaatg cacctgcagc gagaacagta
     3301 agtaaacttt ttttcatggc gttgaatttt ctgtgtcttg tttgatttga gcaaaaatag
     3361 caaaagtgct cctgcttttg aggcaaaaac ggttatctct tacgtgttca gtttgttaaa
     3421 tgaaggccgg tcagacgaac acctgcatgt cgtacagctt gcggtaaacg ccgttgcgtg
     3481 caagcagctc cgcgtggttt ccgcgttcga tgatctcgcc tttctgcagc acgattattt
     3541 catcggcatg ctggatcgtg cttaaacggt gtgcgatgat gagcgacgtc ctgttctgca
     3601 tgagcttgtt cagcgcgtcc tgtacgaggc gttcgctttc cgtatcgagc gcagatgtgg
     3661 cttcatcgag aatgaggatc ggcgggtttt tcagcacggc gcgcgcaatg gcgatgcgct
     3721 gccgctgccc gcccgacatt ttgctgccgc ggtcgccgat gttcgtttcg tagccgtccg
     3781 gcatttccat aatgaaatca tgcgcattgg ccactttcgc ggcggcgatc acgtcttcct
     3841 tcgtggcgga ttccacgccg aaggcgatgt tggcgaggac ggtgtcgttg aaaagaatgg
     3901 attcctgtga aacgatcccc atgagcgcgc gcaggtcgtg aatgcgcagg tcgcctgtgt
     3961 tcgttccgtc gagcagcacc tgcccggacg tcggttcgta aaagcggggc aggaggtcgg
     4021 cgagcgtggt ttttccggaa ccggattgtc ccacgagtgc gatcgttttt cccctcggga
     4081 tcttcaggtt gatgttcttc agcgcatagc cctggtcgcc gcggaggtaa gcaaaggaaa
     4141 ggtcgcggta ctcgatctct ttttcgaagc cggaaacttt tttcgcgtcg cttttgtcgc
     4201 ggatggtttc ttcggcgtga aggatcttgt tgatgcggtc gatggaaaca atgcctttct
     4261 ggatgttgga atacgcgccc gtgaatgttt tggccggcgc gatcacctgc gaaaacgtaa
     4321 tgatgtaaag tacgaacgtg cttccttcca tcgtggcgcc ggcacccacg accagccttc
     4381 cgccgaaata gatcagcacc atcatgatcg cggagctgat gaattcgctc atcggggaag
     4441 ccaggtcgac tttccggtag gtgctgatca tctgtttcgt gtagtgttcg ttcaccttgc
     4501 cgaatttttt attcatgctt ttttcagcgt tgaacccttt gatgatccgc agtccgccca
     4561 gcgtttcttc catgatggag aacagcgtgc ccagctcttc cttcgcccgg ttcgaggaac
     4621 gtttcaggct tttgctgacg gtggaaataa gaaacgccgc caccgggaga agaatgaaaa
     4681 tgaccagcgt aagcgccggg ctgatgtaaa cgagaaaccc gaggatgatg aggatgttca
     4741 ccgggtcgcg gaagatcatt tccagggagc tcatcaccga ccattccact tcctgcacgt
     4801 cggtagtgat gcgcgacatg atgtcgccct tgcgttcatt cgaataatag gaaagcggga
     4861 gccgcaatgt tttttcgtaa atggaattgc gcaggtcttt caccacgccg ttgcggatgg
     4921 gcgccaggaa gaacatgccg aggtaacgga aaagcgcctt gaagaaaaag atcacgccca
     4981 caccgatgca gatcatcacg agcgctttca tttttccttc gtgcatgata agatcgctga
     5041 attcgtagtt gaggtatttc atcaccgaat tgaacgatgc gcccagcgaa ggctttcctt
     5101 cggcgagtat cttctggtaa acgtcatctg atttgaccgt gatgaggtcg aggaacggtt
     5161 tgatcatccc cagtgtgaaa agtccgaaga tgacggaaag aatattgaag aaaatattca
     5221 gccatccgta gcgccagtag cccctgacgt acttaaggac accgaagaag tttttcatcg
     5281 aacgcaaaga taccgtttag aatgaagtgg gagggagaag cgccgtgtgc gcccgggtta
     5341 aaaagtgtga taaaatattc cccgtcctcc gttctccctt ctaactttgc gccatgaaat
     5401 caaagaccgc caaagaatcg ctggccatcg ccacgcacca cgtgcttccg aacgatacga
     5461 acaacctcaa caacatgttc ggcggaagac tgctggaatg gatggacatc atcgctgctg
     5521 tttccgcgca tcgccattgc cgccgcgtgg tggtgacggc gactgtcaac cacgtcgctt
     5581 tccacgaagg aattccgtac gggagcattg tcacgctcca ggcgaaggtg tcgcgcgcgt
     5641 tcaatacgtc gatggaggtt tttatcgatg tgttcgtgga gaaccaggtt tccggcgagc
     5701 agctgaaaac gaacgaagct atttacactt tcgtggcagt cgaccagaac ggctcaccac
     5761 ttcccgtgcc cgagctcgtc ccggaaacgg aggaagaaaa atcccgtttc gacggcgcgc
     5821 tgcgcagaag acagcttgcg cttatcctcg ccggcaaaat gaaacccgac gaagcgaccg
     5881 agctgaaggc gttgttcgtg aaataaattt ttgtgtccgc ccggacgccg gtcgtttccc
     5941 actgtccggt cgagcgtgag tcgagacctg tcagtcgagc gtcagtcgtt tcccttgtca
     6001 ggtcgagcgc gagtcgatcc cattgtcagg tcgagcgcga gtcgagacct gtcagtcgag
     6061 cgccaatcgt ttcccttcgt caggtcgagc gccaatcgtt tcccttcgtc aggtcgagcg
     6121 cgagtcgatc cccttgtcag gtcgagcgcg agtcgatccc cttgtcaggt cgagcgcgag
     6181 tcgatcccct tgtcaggtcg
//
LOCUS       DASTSF010000473         3006 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1858673,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000473 DASTSF010000000
VERSION     DASTSF010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3006
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1858673"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(203..904)
                     /locus_tag="VFU15_13720"
     CDS             complement(203..904)
                     /locus_tag="VFU15_13720"
                     /inference="COORDINATES: protein motif:HMM:NF023504.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718895.1"
                     /translation="MYNQGNFYQQPPQQPQKKGNNRLVLWIVISALITGLLATAVYFA
                     AQMGKKFGTTISNLSEQAMQVSDSLEAQGNEETYMNLRSRIDADSSLAPYASKLDSLQ
                     AWTEAIRAEVLREKDGFKASLGSGQPGFFDKRPAEKYFIATGKATMLKRDLIAYRSRV
                     LNDMPGDIRDTLLKNQIDVDAFAKLSVLGSFAQWERMKFDQPATTVFMNIKMIITQVR
                     NFESSVLSRYSSKIR"
     gene            complement(927..2330)
                     /locus_tag="VFU15_13725"
     CDS             complement(927..2330)
                     /locus_tag="VFU15_13725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718896.1"
                     /translation="MNRKSIHLLLLIALVALTRIPFIFDGYGTEEDSWGLVVNAHEMK
                     ESGHYVASRFPGHPVQEYAYRMICDQPAWVYNFLSVIVSCIAVAFFFLALGKMQLSGK
                     IKTTLLLCFTPAFFIAGTYTIDFNWALAFVLASFYFLLGRKFIIAGIMIGLATGCRIT
                     SGIFLLPWAMLLFNRLDLRAWLKELLLVSIPAVIIGMLLYVPAYLNYGTAFFDYSDQF
                     PYPPVAKVLYKMSIGVWGIPGLTAIALSVFAAVKTIREKNINAPVLFSPQRLAAVCAA
                     IIVLHVVSYLRLPQKSGYMMPAVPFVILLIALFVRGKWLNTVTALFVIAPFLFGMNLT
                     DNLRGSEYSPLAVRFHSSGQEIFFDPLTGPALSERSKRKNKMNYCSEVAASLDTLSSP
                     SCIISGWWYNELVVNYYSQEKNKNATLEFYSTCAHLDSLRKEGVRIYFLPEQDLYNDE
                     MFGQNCTDSIAKPFPVQ"
     gene            2453..2932
                     /locus_tag="VFU15_13730"
     CDS             2453..2932
                     /locus_tag="VFU15_13730"
                     /inference="COORDINATES: protein motif:HMM:NF020967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HEU4718897.1"
                     /translation="MKDTPIRLVTVLTSMHLSELSVICGRLEADGIECHIQDELTAQV
                     NPFYSNAIGGIKLQVEEKDLQKALAVLRSHGYITERDMKPLPLITELDSFSSRLPFLK
                     GVRLEVRLVVIVAIISAIITLFVYVLTLPSVFRHVSTNGAGGGTIPLLKKTLTVSRA"
BASE COUNT          706 a          744 c          885 g          671 t
ORIGIN      
        1 gctgaatggc cttatcggta agacgaataa cgggcgcaga gattttcagg cctgtttttt
       61 tgatgaacgg aagataattc catagcccgg ggcgagacag gtgccccgcc gcacggcgtg
      121 cacaggcgag caccggaaga gaggaactga aatgcgcgga agctgtgctt caaaaaagaa
      181 aacgggaaaa agaaaaactg cctcagcgaa tcttgctgct gtaacgggaa agcacggacg
      241 attcgaaatt gcgcacctgg gtaatgatca tttttatgtt catgaaaaca gtagtggcgg
      301 gctggtcgaa tttcatccgt tcccattggg cgaagctccc gagaacggac agtttcgcga
      361 acgcgtcgac gtcgatctgg tttttcagca gcgtgtcgcg tatgtcaccg ggcatgtcgt
      421 tcagcacgcg ggagcggtaa gcgatgagat cgcgtttcag catggtggct ttacctgtag
      481 cgatgaaata tttttccgcg ggacgtttgt cgaagaagcc gggctggccg gagccgagcg
      541 aagctttaaa accgtctttc tcgcgcagga cctcggcacg gatcgcttct gtccacgcct
      601 gcaggctgtc gagctttgac gcgtagggcg ccagcgacga atctgcatcg atgcgcgacc
      661 gcaggttcat gtacgtttct tcatttccct gcgcttccag cgagtcggaa acctgcatgg
      721 cctgctcgct caggttgctt attgtcgtgc cgaacttttt acccatctgt gcggcgaaat
      781 aaacggcggt ggctaaaagc ccggtgatga gcgcgctgat gacaatccag agaacgaggc
      841 ggttgttccc ctttttctgc ggctgctgcg gtggctgctg gtaaaaattt ccctggttgt
      901 acatgctgtc cggttaaagg acaaagctat tgcacaggga acggttttgc aatgctgtcc
      961 gtgcaattct gcccgaacat ttcgtcgttg tacaggtcct gctcgggaag gaaataaatg
     1021 cggacgccct cctttcgcag cgaatcgagg tgcgcgcagg tggaatagaa ttcgagcgtg
     1081 gcgtttttgt ttttttcctg cgaataataa ttcaccacca gctcgttgta ccaccacccg
     1141 gagatgatgc aggaaggaga agagagcgtg tcgagggacg cggcgacctc gctgcagtaa
     1201 ttcattttgt ttttccgctt ggagcgttcc gaaagcgcgg ggccggtgag cggatcgaaa
     1261 aagatctcct gcccggagga atggaagcgg accgccagcg gggaatattc gcttccgcgc
     1321 aggttgtcgg tgaggttcat gccgaagagg aacggcgcga tcacgaacag ggccgtcacc
     1381 gtgttcagcc attttccgcg tacgaaaagt gcgatgagca gtatgacgaa aggaacggcg
     1441 ggcatcatgt agccgctttt ctgcggaaga cgcaggtagg aaacgacgtg caggacgatg
     1501 atcgccgcgc agacagcggc aagacgttgc ggcgaaaaaa gaacgggtgc gttgatgttt
     1561 ttttcccgga tcgtttttac cgctgcgaaa acggaaagtg cgatcgcggt gagaccggga
     1621 attccccaca cgccgatgga cattttgtac agcactttcg ccacgggagg gtacgggaac
     1681 tggtcgctgt aatcgaagaa cgccgtgccg taattcaggt aagcgggaac gtacagcagc
     1741 atgccgatga tgacggcggg aatggaaacg agaagcagct ccttcagcca tgcgcgcagg
     1801 tcgagacggt tgaacagcag catcgcccat ggcagcagga aaattcccga cgtgatgcgg
     1861 cagccggtgg caaggccgat catgatcccc gcgatgatga atttccggcc gagcagaaaa
     1921 taaaacgacg cgagaacgaa cgcgagcgcc cagttgaaat cgatggtgta cgttccggcg
     1981 atgaaaaagg caggcgtgaa acagagcagc agcgtggttt ttatttttcc gctgagctgc
     2041 attttcccca gcgcgagaaa aaagaacgcg acggcgatgc agctgacgat cacgctgagg
     2101 aaattgtaca cccacgcggg ctggtcacag atcatgcggt acgcatattc ctgcacagga
     2161 tgaccgggga aacgcgacgc gacgtaatgg ccgctttctt tcatttcgtg cgcattcacg
     2221 acaagtcccc aggaatcttc ctccgtgccg tagccgtcga aaatgaacgg gattctcgtc
     2281 agcgcgacaa gcgcgatcag caataacagg tgaatgcttt ttctgttcaa ggtccgcggt
     2341 taatgttctg cgttcaaagt tctgaattta tggattcgat gaatgacccg gatttattcc
     2401 gtattcctgt gtaggtttga ataacatcct gccccgcccg taaaagtttg aaatgaaaga
     2461 cacgccgata cgactggtaa cggttctgac ctcaatgcat ctttccgagc tttccgtgat
     2521 atgcgggcgg ctggaggcgg acggtattga atgccacatc caggatgagc tgacggcgca
     2581 ggtgaacccg ttttattcaa atgccatcgg cgggataaag ctgcaggtgg aagaaaaaga
     2641 tctgcagaaa gcgctcgcag tccttcgcag ccacggctac attaccgaaa gggatatgaa
     2701 gcccttgcca ctgattacgg agctggacag tttctcctcg cggcttcctt ttctcaaagg
     2761 cgtacggctc gaggtgcggc tggtcgtcat cgtcgcgatt atttcagcga tcatcaccct
     2821 gttcgtgtac gttctgacat tgccttccgt tttcaggcat gtttccacga acggcgcggg
     2881 tggcggaact attcccctgc ttaaaaaaac gctgactgtt tcgcgtgcgt aacaaggaag
     2941 aaataagatg cccttcatgc ggttcggacg atgtttcgaa accgcgtatt tcagagcgcg
     3001 cttttg
//
LOCUS       DASTSF010000474         5266 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_302370,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000474 DASTSF010000000
VERSION     DASTSF010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5266
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302370"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..497
                     /locus_tag="VFU15_13735"
     CDS             <1..497
                     /locus_tag="VFU15_13735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718898.1"
                     /translation="VLIAEQYYVIENASTSVSNGKTYTEIHYSYYYNDVMALNFNGSG
                     GLDWKAKIAKRQRSEDDGGLYSSYLPYYKDNKLYIIYNDTPKNQMMKPGGAPEYFIPG
                     KDCQVVMAVIDEKGNVSRESIPGISDGKFMICPSSSKVYPGAEIMLYLSDFKSYKFAR
                     VNIK"
     gene            508..1158
                     /locus_tag="VFU15_13740"
     CDS             508..1158
                     /locus_tag="VFU15_13740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718899.1"
                     /translation="MFSPKTFLISAAFFLCAMVHAQDFGFSGSWFTVMPVGDTSHVGA
                     QPGFRVEGTIWNSDFGFPRLCTQSITYGQTFPVTIEKVAYLHNNLPSTGKINFSTRSF
                     GVRYTTEAAQPTKHLAVHIGLGLYMTSILYTFKPEGVLSPGDYATDFLGHEYSYGNPS
                     QKTFSGMFELTAGVSYEFRPFNIFVNAGFYRGLFSEMPFQNSIDLAAGIFVPVWRR"
     gene            complement(1246..3237)
                     /locus_tag="VFU15_13745"
     CDS             complement(1246..3237)
                     /locus_tag="VFU15_13745"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HEU4718900.1"
                     /translation="MKGKAIIIIVLLLGSLQSLNAQHRLGRAGYFKTFDYVRLAHSLT
                     DNVESDSLKVKSIYLWMTKHIRYDMGGYFSGSGSYTDPNDILRHRTAVCLGISILFDS
                     LCSVAGIQSEVVWGYVYEPWYEGRDTIFLDTHAWNVVQIDGEWKLVDVTWAAGSLQQR
                     RQPLRRLLWKWFKIPYGIKYRNKRKRNDFYYCTPPEKFVLDHLPSTPAWQLLDCSVPM
                     DSFQMSPTATQHFLDAPVNCRNGNDSIAAITGAVSYKKWMVSGQQALAFNKHNHQDIS
                     FGMRDYGYYLFTQAEDTDYDYKTRIAYYDSCLAQLDSMSKSFLATAKDCRDEGAFFVR
                     RNKRMNRQLLNEDKPLIKKKSAEERDWRKQRQNARRMVRKLQSENKKLGKASTYYSRK
                     RIKISRPKKPNDKEADRIDKLVDHLGDMDDRISDLTDSASNVAFPLFADLAPQYMAMI
                     DTLDSCAYVQLIYQRSLVVLRAYYFNSFDTVVIVRKDHVMKLQHEEDSIRSIIPGAGM
                     WAADSAANVYKKNVKKIQLTLKDEMQACSQLGKMSKSGYDEKQKYIDCRRQYVAWNDS
                     VISYNNQLIRQYNDYAGSLGSLEKKAERSAKYFQYEVNYENMRFRSTTVFFRMYYGGH
                     ANSMKMNSDTCKKLKRIVASRKKQLEAKHRKEMQQKAKS"
     gene            complement(3343..4068)
                     /locus_tag="VFU15_13750"
     CDS             complement(3343..4068)
                     /locus_tag="VFU15_13750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009196312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU4718901.1"
                     /translation="MGRIFEKRKHKMFKRYATMAKQFTKIGKEIAIAVKAGGPDPATN
                     ARLRLVIQNSKAVNMPKDRVDNAIAKAAGKNDSSNFEEIVYEGKGPGGVSVVVETATD
                     NPTRTVANVRVYFNRGGGELGKSGSTDYLFERKGVFRINTTGQNMDELELELIDAGLD
                     SIEPGDENDAMVYCAFSDFGSMQKALEAKNIEVLEAKLTRIPGSYVSLNAEQLAEFQK
                     LLDKLEEDEDVQEVYHNMAEAEE"
     gene            complement(4120..>5266)
                     /locus_tag="VFU15_13755"
     CDS             complement(4120..>5266)
                     /locus_tag="VFU15_13755"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008511369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S16 family serine protease"
                     /protein_id="HEU4718902.1"
                     /translation="AGSSNPVFILDEIDKVGNDWHGDPSSALLEVLDPEQNHAFYDNY
                     IELDYDLSKVMFIATANSLSTIQPALRDRLEIIEVSGYTTEEKLQIARRHLVPKQLEE
                     HGVKKSQLSLSDAVIEFIIENYTRESGVRGLEKMIAKMIRYAAKNIAMNQKYNARPGI
                     PDLQKILGPAHEKDRYQGNDVAGVVTGLAWTPVGGDILFIETSLSKGKGKLTLTGNLG
                     EVMKESAVIALEYLRAHGYQFGINPDLFDHWNVHIHVPEGATPKDGPSAGIAILTALA
                     SAFTQRKVKKNLAMTGEITLRGRVLAVGGIREKILAAKRAGIREIILCIENKKDVEEI
                     NKDYLKGLHFHYVENMVDVLKAALLKEKVKNPVDLVIPQEARLKKVD"
BASE COUNT         1122 a         1472 c         1292 g         1380 t
ORIGIN      
        1 ccgtccttat cgccgaacaa tattacgtga tcgagaatgc gtccacctcc gtgtcaaacg
       61 ggaagacata cacggaaata cattactctt actattacaa tgacgtgatg gcgctcaact
      121 tcaacgggag cggcggactg gactggaaag cgaagatcgc gaagcgtcag cggtccgagg
      181 acgacggcgg tctttactcc tcttacctgc cctattataa agacaacaag ctttacatca
      241 tttacaacga cacaccgaaa aaccagatga tgaaaccggg cggcgctccg gaatatttta
      301 tacccggtaa agattgccag gtagtgatgg cggtgatcga cgaaaaggga aatgtttcgc
      361 gtgaaagcat acccggcatt tccgacggaa aattcatgat ctgtccttcc tcgagcaaag
      421 tatatcccgg cgccgagatc atgctttacc tctcggattt caagagctat aaattcgcac
      481 gcgtcaacat aaaataatcc tgcagccatg ttctcaccga aaacattttt gatctccgcc
      541 gcatttttcc tttgcgcgat ggtacatgcg caggacttcg ggttcagcgg ttcctggttc
      601 accgtcatgc cggtgggcga cacttcgcat gtcggcgcac aacccggttt ccgggtggaa
      661 ggcacgatct ggaattccga tttcggcttt ccgcgcctgt gcacgcaaag catcacctac
      721 gggcagacct ttcccgtcac aatcgagaaa gttgcgtacc tgcacaacaa ccttccctcg
      781 accgggaaga tcaatttttc cacgcgctct ttcggcgtgc gctacaccac cgaagccgca
      841 caaccgacaa aacatctcgc ggttcatatc ggtctcgggc tgtatatgac ctcgatcctc
      901 tacaccttca aacccgaggg cgtcttgtct cccggtgatt atgcgacgga ttttctcggc
      961 catgaatatt cctacgggaa tccttcgcag aaaacattca gcggaatgtt cgagctaacg
     1021 gccggcgtct cgtatgaatt ccgcccgttc aatattttcg tcaatgcagg attttaccgc
     1081 ggactgtttt cagagatgcc gttccagaac agcatcgacc ttgcagcggg gatttttgta
     1141 cctgtctggc gccggtaacc agtgatcttt tgcaaaaccc gggtacacaa atccattccc
     1201 ggttttcctt tccccgatcc gggaaagctg gcataaaaaa aacgttcagc ttttcgcctt
     1261 ctgctgcatt tccttcctgt gttttgcttc cagctgtttc ttccgtgaag cgacgatccg
     1321 tttcagcttt ttgcaggtat ccgaattcat cttcatgctg ttcgcatgac cgccgtaata
     1381 catgcggaag aaaacagtag tgctccggaa acgcatgttc tcgtaattca cttcgtactg
     1441 aaagtatttc gcggaacgct ccgctttttt ttcgagactt cccagtgaac ccgcgtaatc
     1501 gttgtattgc cggatcagct ggttattgta gctgatcacc gaatcgttcc acgccacgta
     1561 ctgcctgcgg caatcgatgt atttctgctt ttcatcgtac ccgcttttgc tcatcttgcc
     1621 gagctggctg cacgcctgca tttcgtcctt gagcgtgagc tgtatctttt ttacattttt
     1681 cttgtaaaca ttcgctgcgg aatcggccgc ccacatcccc gcacccggaa tgatcgaacg
     1741 gatgctgtct tcctcatgct gcagcttcat cacgtgatct ttgcgaacga tcaccacggt
     1801 atcgaagctg ttgaaataat acgcccgcag cacgacaagg gaacgctggt atataagctg
     1861 tacatacgcg cagctgtcga gcgtatctat catcgccatg tactgcggag ctaaatccgc
     1921 gaaaagcggg aatgccacgt tcgaagcgct gtcggtgaga tcgctgatcc tgtcatccat
     1981 atcgccgaga tgatcaacga gcttgtcgat acggtcagct tccttgtcgt tcggtttttt
     2041 cggccgtgaa atttttatcc gtttccgcga ataatacgtc gatgctttgc cgagcttttt
     2101 gttctcgctc tgcaatttcc tcaccatgcg ccgcgcattc tgccgctgct tcctccagtc
     2161 tctttcttca gccgatttct ttttgatcag cggtttgtct tcattcagca gctgccggtt
     2221 catgcgtttg ttccgccgga caaaaaaagc accttcatcc ctgcaatctt tcgccgtggc
     2281 gagaaacgat ttcgacattg aatccagctg cgccaggcag gaatcataat aagcgatccg
     2341 tgttttgtaa tcgtaatccg tgtcttcggc ctgcgtaaaa agataatacc cgtaatccct
     2401 catcccgaat gaaatatcct ggtgattgtg cttgttgaaa gccagcgcct gctgtcccga
     2461 caccatccat tttttgtacg aaaccgcacc ggtgatcgcg gctatgctgt cattgccgtt
     2521 cctgcagttc accggggcat cgaggaaatg ctgcgtggcc gtcggcgaca tctggaatga
     2581 atccatgggg acggaacagt cgagcaactg ccaggccggc gtggacggaa gatgatcgag
     2641 gacgaatttt tccggcggcg tgcagtaata aaaatcattc cgcttccgct tgttcctgta
     2701 cttgatgccg tagggtatct taaaccattt ccacagcagc ctccgcagcg gctgcctgcg
     2761 ctgctgcaat gatcccgcgg cccacgttac gtcaaccagc ttccattccc cgtcgatctg
     2821 cacaacattc caggcgtgcg tatcgagaaa gatcgtatcg cggccttcgt accagggttc
     2881 gtaaacatat ccccagacga cttcggactg gattcccgcg acactgcaca gcgaatcgaa
     2941 aagaatgctg ataccgaggc aaacggccgt gcggtgacgc aggatatcat tcggatctgt
     3001 atagcttccg gagcccgaaa aatatccgcc catgtcgtaa cggatgtgct tcgtcatcca
     3061 caggtaaatg ctcttcactt tcagcgaatc gctttccacg ttgtcggtga gcgaatgggc
     3121 aagcctgacg taatcgaatg ttttgaaata gcccgccctg cccagcctgt gctgcgcgtt
     3181 aagcgactgc aggctgccca gcaaaagcac gatgataatg atcgcttttc ctttcatacg
     3241 gtacgggaga acagggttcg tccgccggag ttatttcaaa tatacagacg caaaaaaagc
     3301 cccggaggtt gccggggccg gttcttttca cgaaaaaact tatcattctt cggcttccgc
     3361 catgttatga tatacttcct gcacgtcttc atcctcttcc agcttatcga gcagcttctg
     3421 gaattcggcc agctgttccg cattcaggga aacataggaa ccgggaatgc gcgtgagctt
     3481 ggcttcgagc acctcgatat ttttcgcttc gagcgctttc tgcattgagc cgaaatcgga
     3541 aaatgcgcag taaaccatcg cgtcattctc atcgccgggt tcgatgctgt ccagtccggc
     3601 gtcgatgagc tccagctcga gctcgtccat gttctgtccc gttgtgttga tgcggaaaac
     3661 gcctttacgc tcgaagagat aatctgtcga tccggatttt ccgagctctc cgccgccgcg
     3721 gttaaaatat acgcggacgt tcgccactgt ccgggtggga ttatccgttg ctgtttcaac
     3781 aacaacgctt acaccgcccg ggccttttcc ttcgtagacg atctcttcga aattcgacga
     3841 atcgtttttt cccgccgcct tcgcgatcgc gttatcgacg cggtctttcg gcatgttcac
     3901 ggctttcgag ttctggatga caagacgcag gcgcgcgttc gtcgcaggat ccggtccgcc
     3961 ggctttcacg gcaatggcga tctccttgcc gatcttggtg aactgcttcg ccatcgtggc
     4021 gtagcgtttg aacattttat gcttccgctt ttcgaaaatg cgtcccatat caatgaaatt
     4081 tcaggtgaag tgcaaagata gcaattgaag aatttccggt taatcgacct ttttcagcct
     4141 tgcttcctgc gggatgacaa gatcgaccgg gttcttcact ttttctttca gcaatgcggc
     4201 cttcagcacg tccaccatgt tttccacgta gtggaaatga agccctttca ggtaatcctt
     4261 gttgatctcc tcgacatctt ttttgttctc gatgcagagg atgatctccc tgatcccggc
     4321 gcgtttggcg gccagtatct tctcacgtat ccccccaacc gcaagcacgc gtccgcgcag
     4381 cgtgatctcg ccggtcatgg ccaggttctt tttcactttc cgttgcgtga acgcgctggc
     4441 cagcgccgtg agaatggcaa tgccggccga aggtccgtcc ttcggcgttg cgccttcagg
     4501 cacgtggatg tgcacgttcc agtgatcgaa caggtcggga ttgatgccga actggtaacc
     4561 gtgcgcgcgc aggtactcga gcgcaatgac ggcgctttcc ttcatcacct cgccgaggtt
     4621 tccggtgagg gtgagcttcc cttttccttt gctcaggctt gtctcgatga ataaaatatc
     4681 gccgccgacg ggcgtccagg cgagaccagt aaccacaccg gccacatcat ttccctggta
     4741 cctgtccttt tcatgcgcag gaccgagtat tttctgcaaa tcgggaatgc cgggcctggc
     4801 attgtacttc tggttcatcg caatgttctt cgccgcatag cgaatcattt tcgcgatcat
     4861 tttttcgaga ccgcgcacac cggattcacg cgtgtaattc tcgatgatga attcgatgac
     4921 ggcatcggaa agcgagagct ggcttttctt cacgccatgc tcttcgagct gcttgggcac
     4981 aagatggcgg cgtgcgatct ggagtttttc ctccgtcgtg tagccgctca cttcgatgat
     5041 ctcgagacgg tcgcgcaaag caggctgtat cgtcgaaagc gaattcgccg tggcgatgaa
     5101 catcactttc gaaagatcgt agtcgagctc gatgtaattg tcgtagaacg catggttctg
     5161 ctccgggtcg agcacttcga gcagcgcgga cgaagggtcg ccgtgccagt cattgcccac
     5221 cttgtcgatc tcatcgagaa tgaaaaccgg gttggacgag cctgct
//
LOCUS       DASTSF010000475         2495 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1876307,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000475 DASTSF010000000
VERSION     DASTSF010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1876307"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..405
                     /locus_tag="VFU15_13760"
     CDS             <1..405
                     /locus_tag="VFU15_13760"
                     /inference="COORDINATES: protein motif:HMM:NF045201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HEU4718903.1"
                     /translation="CMSVGRGTDTPYQVVGHPDLKNAAYTFTPASRRGAKHPPYEGQV
                     CHGHDLHEFATMYVRDYKGLYLFWLLGCYKDMDNKDQFFNSFFEKLAGTKTLRQQVID
                     GKTEDQIRASWRPGIAKFKVIRKKYLLYEDFE"
     gene            499..2076
                     /locus_tag="VFU15_13765"
     CDS             499..2076
                     /locus_tag="VFU15_13765"
                     /inference="COORDINATES: protein motif:HMM:NF013264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEU4718904.1"
                     /translation="MKKLYTLFSFSLICSLANAQTGPYWATDVAPILYANCTTCHHQG
                     GIAPFSLMTYNDAVLNSSGMVYDITHGVMPPWPPDTNYTRFCNERKLSASQIQTISDW
                     VNNGAQQGNSSQAPTPPTYTNTPVLANADLVLQMPAYTVNTPTDLYRCFVMPSGISQN
                     EYITKMEVIPGNRSCVHHVLVYQDQSSTCINLDNNDPGPGYTWFGDVGSSTATLIGGW
                     VPGQGVWELPPNMGVELMANTNIIMQIHYPGGTFNQTDSTQVRFTFSTGVVRTVTLNP
                     VINHFTSLTNGPLHIPADSVKTFYAQENVLMNSTLISIAPHMHLIGHSMINYAVDPSG
                     DTIPLIDINNWSFHWQGFYTFRQPVHVPYGSVLYARAVYDNTSNNPENPNTPPADVWA
                     GESTTDEMYIIYYAYLPYQPGDENIIVDSTIIQGVQQQSFSDYVKTPQLYDAYPVPAA
                     GNSVLNISYFLPEESKVTVDLTDMSGKVVSVPVKDENTSAGFTSKQVSTDGLAAGTYL
                     LRLTTDGIVKTKTIVIE"
     gene            complement(2167..>2495)
                     /locus_tag="VFU15_13770"
     CDS             complement(2167..>2495)
                     /locus_tag="VFU15_13770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEU4718905.1"
                     /translation="IVFGLKYTVNRPRPFVTYPDIIKKTKAGSKSFPSGHTSSAFALA
                     TSVSLAYPKWYVIAPSYAWACAVGYSRMRLGAHYPSDVAAGALIGAGCAWGCWWLNKK
                     IQEKYF"
BASE COUNT          591 a          708 c          647 g          549 t
ORIGIN      
        1 tgcatgagtg tcgggcgcgg aacagacacg ccttaccagg tggtcggtca cccggatctg
       61 aagaacgcag cgtatacgtt cacgccggct tcacgccgcg gggcaaagca tccgccgtac
      121 gaaggacagg tttgccacgg gcacgacctg catgaattcg caacgatgta tgtgcgtgat
      181 tacaaggggt tgtacctgtt ctggctgctc ggctgctata aggacatgga caacaaggat
      241 cagttcttca acagcttttt cgagaagctt gccggtacaa aaacattgcg ccagcaggtg
      301 atagacggaa aaacggaaga ccagatccgc gccagctgga ggcccggcat cgcaaaattc
      361 aaggtgatac ggaagaagta tttgctgtac gaggattttg agtaatgcat taaacttttt
      421 ccctttattg gattccaaca ggacggatgc ctggtaccgc tacatttcgt acctttcatt
      481 ctgcaaatga tttccccgat gaaaaaactg tatactctct tttctttttc gctgatctgt
      541 tccctggcaa atgcgcagac cggtccttac tgggcaacgg acgtggctcc cattctttat
      601 gcgaactgca ccacctgtca tcaccaggga ggaattgcgc cgttctcgct gatgacctac
      661 aacgacgccg tgctgaattc ctccggaatg gtgtacgaca tcacccacgg tgtcatgcct
      721 ccgtggccgc cggataccaa ctacacgcgt ttctgcaatg aacgcaagct ttccgcctcg
      781 cagatccaga cgatctccga ctgggtgaac aatggcgcgc agcagggaaa tagttcccag
      841 gcgccgacac ctccgactta tacgaataca ccggtgctcg cgaatgcaga tctcgtcttg
      901 cagatgcccg cttacacggt aaacacaccg accgacctgt accgttgttt cgttatgcct
      961 tccggtattt cgcagaatga atacatcacg aagatggaag tcattcccgg caaccgcagc
     1021 tgtgtgcacc acgtgcttgt ttaccaggac cagtccagca cctgcatcaa cctcgataac
     1081 aatgaccccg gcccgggtta tacctggttc ggcgacgtag gttcttccac ggcgacgctt
     1141 atcggcggat gggtgcccgg gcagggcgtg tgggaacttc ccccgaacat gggcgtggag
     1201 ctcatggcca acaccaacat catcatgcag atccactatc ccggcggaac gttcaaccag
     1261 acggacagca cgcaggtgcg cttcacgttt tccacgggcg ttgtgcggac cgttacactc
     1321 aaccccgtga tcaatcactt tacgtcgctc acgaacggac cgcttcatat tcccgccgac
     1381 agtgtgaaaa cattttacgc gcaggaaaat gtgctgatga actcaacgct tatcagcatt
     1441 gcgccgcaca tgcacctgat cgggcacagc atgatcaatt atgccgtcga tccttccggc
     1501 gatacgatcc cgctcatcga catcaacaac tggagcttcc actggcaggg gttctatact
     1561 ttccgccagc ctgtgcacgt tccgtacgga agcgttctgt atgcgcgcgc cgtttacgac
     1621 aataccagca acaatcccga aaacccgaat acgccgcctg ccgatgtgtg ggcgggtgaa
     1681 tcgaccacgg atgaaatgta catcatctat tacgcttacc tgccttacca gccgggcgat
     1741 gagaacatca tcgtggactc gacgatcatc cagggcgtgc agcagcagtc gttcagcgat
     1801 tacgtgaaaa cgccgcagct gtacgatgcc tatcctgtcc ccgctgcagg caattccgtg
     1861 ctgaatattt cgtatttcct cccggaagaa tcgaaggtaa cggtcgacct cacggacatg
     1921 agcgggaaag tggtttcggt tcccgtaaaa gatgaaaaca cttccgccgg ctttacgtcg
     1981 aagcaggttt cgaccgacgg actcgctgcg ggaacttacc tgttgcggct gacaacggat
     2041 ggaatcgtga aaacgaaaac gatcgtgatc gaataaagcg gacgaacctt atcctgtgcc
     2101 cgcctgtgta agaacaggcg ggttttttta ttctgcattt gtgactgcga aaaatcgcgc
     2161 ccggtctcag aaatactttt cctggatctt tttattgagc caccagcatc cccatgcgca
     2221 cccggcgccg atgagcgcac cggcagcgac atcgctcggg taatgtgcgc ccaggcgcat
     2281 gcgtgaataa ccgaccgcgc acgcccacgc atacgaaggc gcgatcacgt accatttcgg
     2341 gtaggcgagc gaaacagaag tggccagtgc aaatgccgaa gacgtgtgac cggacggaaa
     2401 tgatttcgag cccgcttttg tttttttgat gatgtcagga taagtcacaa agggccgcgg
     2461 gcggttcacc gtgtatttga gcccgaacac gatca
//
LOCUS       DASTSF010000476         4196 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1196093,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000476 DASTSF010000000
VERSION     DASTSF010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4196
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1196093"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2234)
                     /locus_tag="VFU15_13775"
     CDS             complement(<1..2234)
                     /locus_tag="VFU15_13775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718906.1"
                     /translation="MAAALLTGPAVFAQVAAPGWVNAIGTGGGFYENGGGIRAGSNGK
                     IYLGYRYAGSINLNGASPSAAGGGTACGSAQLTQNLSGTGQWATRLQSSGGYDYVFGY
                     NIDVNNNTYTVGTVNSGGALYVAKTTSTGTFINSWNFSTAYGVGANGVCADAAGNMYI
                     AASISSNQTFGTFPLTYQGGGDDMVLMKLNSAGTVQWAIDYGGTGDDECRAIEMDMNG
                     NLWVEGGYTGTPTFGTFTAPAAGSYSNLFVAEFNPNTGACMNLFTAVNAGIGAGAWYE
                     ESDLRIDSCNNIYVAGHFKNTATWGTFSATSVGAEDFYVTKISPTGTFEWVRTGGSAG
                     TDEATGIALDKNWDVLVGGYFSGTMTLGSTSVTATGNNDAFVAKYASGNGALMFVQKG
                     GGVGYEDDYGGLTADDNRQVYLTGGFSDNNVAANTTQFGATNLGNGYYGDIYVAKLDS
                     TPNLRIYPQPQSSYCSGGCYTMPFTTVGTFNGGNIFTAELSDANGSFASGTTTLGTVT
                     ASGPGTISVCIPPSVAAGSNYKIRITSSSPAYSSVVRCGTVTINGPPSITVSPNASIC
                     SGGNTTLTSSGASTYSWTPSSTLSSGTGSSVTATPTATTTYSVIGTDANGCTDTAMTT
                     VTISNSLVVTVSGNTTICSGNSTTLTASGGGNYAWSSGGTSASETVSPASNTTYTVTV
                     TDPVSGCSDTAQVSVLVNATPVAAVSGNNTICAGASTTLTASGGATYAWSTGGTNAAE
                     TVTPA"
     gene            complement(2477..4009)
                     /locus_tag="VFU15_13780"
     CDS             complement(2477..4009)
                     /locus_tag="VFU15_13780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718907.1"
                     /translation="MGRTASDDLFRLVKSLHRSEKRYFRIFSKRHVIGDGNNYVRLFD
                     AIDRQAEYNEKELLEKLPFLRPRLMADQKNHLYWQVLRSLVVFSSGNSIGSQLRFRLS
                     SFDFLYAKGLYVQCGKVIAKTKSLAIHYEKHLVVLECLEREKKLELQLFNLKSSPKKI
                     ARINAEREAAIAKYTLENAYGRLSEEMFFIMSEEMAVRSEKNKKRSRAILNDPLLQKA
                     PAGGTLHSLFNYHRTLSLYCYSHSDFRGFYFHCRAIVKLMDANRDVLAESPKNYIVAI
                     QNLLIAQKNLRLYAALFRTLRKLKLFEAGSESLRAQVFAITHDIELTIYIDTGQFEKG
                     ALLADAITRGLDQYRNYLSSRYGLLFCFNVAYNLIGCEKFRAALNWLNKVINFPGAQS
                     AMPDIFQTANIISLIAHYELGHSDLLDYRISAMTKTFRKKHALYELESLVLSILSRAV
                     VAPPEKRKRLFAELAKKLARLHRDPEKRRAFSSFDFLTWAKSKASEKTMSEVIRSRGK
                     RS"
BASE COUNT          859 a         1133 c         1214 g          990 t
ORIGIN      
        1 gccggtgtga ctgtttctgc ggcgttggtt ccaccggtac tccacgcgta agttgcgcct
       61 ccagacgcgg tgagcgtggt ggacgcgcct gcgcagatcg tgttgttccc gctgaccgcc
      121 gcaacgggtg tcgcgttcac gagcacggaa acctgcgctg tgtcggaaca accggaaacc
      181 gggtctgtta ccgtgaccgt gtacgttgta ttggatgccg gtgacacggt ttcgcttgcg
      241 gatgttccgc cgctgctcca tgcgtagttt cctccgccgg atgcggtgag cgtggtggaa
      301 tttccgctgc agatcgtggt gttgccggaa acggtaacga cgagagaatt ggagatcgtc
      361 acggttgtca tggctgtgtc cgtgcatccg ttcgcatctg ttccgatgac ggaataggtg
      421 gttgttgcag tcggcgtggc cgtaacggat gagccggtgc cggagcttaa cgtcgatgaa
      481 ggcgtccatg aataagtgga tgcgccggat gaagtcagcg tagtgtttcc gcccgaacag
      541 atcgaagcgt tcggtgaaac ggtgatggac ggcggaccgt tgatggtgac ggtgccgcaa
      601 cggaccacgc tgctgtatgc cgggctcgac gaagtgatgc ggatcttgta attgctgccg
      661 gcagcaacgg aagggggaat gcagacgctg atcgttcccg gaccggaagc ggttaccgtg
      721 ccgagcgtgg ttgttccgct ggcaaaactt ccgttcgcat cggaaagttc ggcagtgaaa
      781 atatttcctc cgttgaacgt gcccacggtt gtgaacggca tcgtgtagca accgcccgag
      841 caataggagc tttgcggctg cggataaatg cggaggttcg gcgtggagtc gagcttcgct
      901 acataaatgt cgccgtaata accgttgccg aggttcgtgg cgccgaactg tgttgtgttt
      961 gccgcgacgt tgttatcgga gaatccgccg gtgagataca cctgccggtt atcgtcggcg
     1021 gtaagtccgc catagtcgtc ttcataacca acgccgccgc ctttctgcac gaacatcagc
     1081 gccccgttcc cgctggcgta tttcgcgacg aatgcatcgt tgttccccgt tgcggtaaca
     1141 gaggtcgaac cgagcgtcat tgttccgctg aaataaccac ccacgagcac atcccagttt
     1201 ttgtcaaggg caatgccggt cgcttcatcc gttccggcgc tcccgcctgt acgcacccat
     1261 tcaaatgttc ctgtgggaga gatcttcgtt acgtaaaaat cttcggcacc gacgctggtg
     1321 gcggagaatg ttccccacgt ggcggtattt ttgaaatgac ctgcgacgta aatattgttg
     1381 cacgaatcga tgcgcaggtc gctttcttcg taccaggcgc ccgcgcctat gccggcgttc
     1441 actgccgtga aaagattcat gcacgctcct gtgttgggat tgaattctgc aacgaaaaga
     1501 ttcgaatatg aaccggcagc cggtgcggtg aatgtcccga acgtcggcgt cccggtgtac
     1561 ccgccttcca cccaaagatt tccgttcatg tccatttcaa tggcgcggca ttcgtcatcg
     1621 cctgttccgc cgtagtcgat cgcccactgc acggtgcctg cgctgttcag cttcatcagc
     1681 accatgtcgt cgcctccgcc ctggtaagtc agcggaaacg ttccgaacgt ctggttcgag
     1741 ctgatgctgg cggcgatgta catgtttccc gccgcatcgg cgcagacgcc gttcgcaccg
     1801 acaccgtagg cggtgctgaa attccatgaa ttgatgaacg tcccggtcga cgtggtcttc
     1861 gccacataga gcgcgccgcc gctgttcacc gtgcccacgg tataggtgtt gttgttcacg
     1921 tcgatgttgt aaccgaacac gtagtcgtat ccgcccgaag actggagacg cgtggcccac
     1981 tggcccgttc cggaaagatt ctgcgtcagc tgagccgatc cgcaggctgt tccgcctcct
     2041 gccgctgaag gcgatgcccc gttgagattg atcgatcctg cgtaacggta gccgagatag
     2101 atcttcccgt tgctgccggc acggatgccg ccgccgttct cgtaaaatcc tcctcccgtt
     2161 ccgatcgcat tgacccaacc gggtgcggct acctgcgcga agacagccgg tccggtcagc
     2221 agggccgcgg ccatcagttt tgccagtgac gaccgtagcg acgatgaatg ctttgtaaac
     2281 atggtgtttg cgttttgtta ccccaaagct actttgcatc ggaggtgcgt cccgggaaaa
     2341 gaaaagcaat taacaggata tcaaaaagaa gaaaatgaag atatcatgac cgttttacga
     2401 atgaaaaata ttcttcctgt ttaaaattta caggatgtct gacaggacat aaacgtacgg
     2461 gcaggaaagc agcggatcag cttcgtttgc cgcgtgagcg gattacttca ctcatcgttt
     2521 tttccgaagc tttgcttttc gcccaggtga gaaaatcgaa ggaggagaag gcgcgccgtt
     2581 tttcgggatc acggtgaagc cgggccagct ttttggccag ctcggcgaaa agacgtttgc
     2641 gtttttccgg cggagcgaca accgcccgcg aaaggatgct cagcacgaga ctttccagct
     2701 cgtacaacgc atgtttttta cggaaggttt tcgtcattgc gctgatgcgg tagtccagca
     2761 ggtcggaatg accgagctcg taatgcgcga tgagcgaaat gatattcgcc gtctggaaaa
     2821 tatccggcat cgccgattgc gcgccgggaa agttgatcac tttgttcagc cagttcagcg
     2881 ccgcgcggaa tttttcacag ccgatgaggt tatacgccac attgaagcag aacagcaggc
     2941 cataacgtga tgaaaggtaa ttcctgtact gatcgagtcc gcgtgtgatc gcgtcggcga
     3001 gcaaagcgcc cttttcaaat tgtcccgtgt cgatgtagat cgtcagctcg atgtcgtgtg
     3061 ttatggcgaa cacctgcgcg cgcagcgatt ccgaacccgc ctcgaacagc ttgagcttgc
     3121 gcagcgtgcg gaacagggcg gcgtagaggc ggaggttctt ctgcgcgatg agcaggttct
     3181 ggatcgcgac gatgtaattt ttcggcgatt cggcgagcac gtcgcggtta gcatccatca
     3241 gctttacgat ggcgcggcag tggaaataaa atccgcggaa atcggagtga ctgtagcagt
     3301 aaagactgag cgtacggtga taattgaaaa ggctgtgcag cgttccgccc gccggcgctt
     3361 tctgcaggag cggatcgttg agaatggcgc ggctccgctt tttatttttc tcgctgcgca
     3421 ccgccatctc ttccgacatg atgaagaaca tttcttccga aagccggcca taggcgtttt
     3481 caagggtgta tttcgctatc gcggcttccc gttccgcatt gatgcgcgca atctttttcg
     3541 gtgagctttt caggttgaac agctgcagct cgagcttttt ttcccgctcg aggcattcca
     3601 gcaccaccag gtgcttttca taatggatag caaggctctt cgttttcgcg atcactttcc
     3661 cgcactgcac gtacaaccct ttcgcgtaca ggaaatcgaa gctgctgagg cggaaacgca
     3721 gctgcgaccc gatggaattc ccggaagaaa aaacgacaag cgaacgcagc acctgccagt
     3781 acaggtggtt cttctggtcg gccatcaggc gcggacgaag aaagggcagc ttttccagga
     3841 gctctttttc attgtactcc gcctgccggt cgatcgcatc gaacaggcgg acgtaattgt
     3901 tcccgtcgcc gatcacgtgc cttttcgaaa agatccggaa gtagcgtttc tcggaacggt
     3961 gaagcgattt caccagccgg aacaggtcgt ctgaagcggt ccttcccatc tgcgtgcgtt
     4021 tgcaatgatc tgctgaaact acgaaaaaca cgacgcccgt acgcggccgg ctgcaaattc
     4081 gcctgaagcc tgttaccttt accggacaat cgcaggttca tgaaaaatct tctccgctat
     4141 tttgtccagg gacttatcgt actggtgccg acggcgatca cggtgcttgt tttgta
//
LOCUS       DASTSF010000477         7606 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_81749,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000477 DASTSF010000000
VERSION     DASTSF010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 7606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7606
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81749"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..863
                     /locus_tag="VFU15_13785"
     CDS             <1..863
                     /locus_tag="VFU15_13785"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEU4718908.1"
                     /translation="IFLYQDANTLINRPYLIVDWGDMTTDTCNAGTSVAITPNVLKTT
                     YSMPVHSFPHSGGYDVNVADTFLVPNIENVASINNYLYIHTYYVVSPTLSNNTPYFIN
                     DQTVITNSNGTYYHSASAVDSNGDSLAYYLQYPWGVSGYVPPNATIDPYTGLVTFFPY
                     STGLYLIPIGVEEYRDTGTGVQLISITEREMIIEVNSLASVSENDFVPHTLTVFPNPA
                     KTSATFQLSGQIQMFELIITDQLGREVWRGNSDSDRLQLSTENFLPGMYFCRIEEQGE
                     ITASGKLIIE"
     gene            1141..1485
                     /locus_tag="VFU15_13790"
     CDS             1141..1485
                     /locus_tag="VFU15_13790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718909.1"
                     /translation="MDKEKLIELAKERLQTLNKIHNLLAIGTLVLCGLPVIATVIIIW
                     ISDSKTLLYPCSVFVVIGLLIYFFIERAKRKALTGAAKFRYELDLFMKGGRGYEGGDS
                     IKKFQDFLTNER"
     gene            1795..2346
                     /locus_tag="VFU15_13795"
     CDS             1795..2346
                     /locus_tag="VFU15_13795"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEU4718910.1"
                     /translation="MNTVFPSQFPHVLTQRLALGKITGSDAHALYDLYSDERVMKFHG
                     RPVLGAAEETEKIIFGWRRLFATEKGIRWGIRFRGGEKIIGTIGVRCSAEHRRAETGY
                     ELAPALWNQGIMTEALGAVTDYVFTQMNFETIEANIAPGNTASQRVLEKAGFRQEAHF
                     RSNYFYEGWWDSVVFSLSRETRR"
     gene            complement(2401..3798)
                     /locus_tag="VFU15_13800"
     CDS             complement(2401..3798)
                     /locus_tag="VFU15_13800"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEU4718911.1"
                     /translation="MKRKLLYALLLLFSAAATAQVSVPNGGFESWISNTYYEPQYYVT
                     SNANVHQLGYPAPNVTRTTDAQLNTYAINLQTVSNGQDTVFGFFSNGDPSNSSGGIPY
                     AQRPTALTGYYKCNVPAGDTALVLAVFKLNGSVIGGGIQKITGTASTYTMFSLPVSLP
                     PVVVPDSVIIAAASSNALVFNGIPGSMLQLDNLSFTGVATQPAMMNGDFELWTSFTTV
                     TPNSWLMFGDSAMQTTDHYTGQYAVKLGTYFDSQSNATPSYVTNGRPVPNALPDGGFP
                     YSLQIDTLVGAYKFLSPGLDTAIGVAHFSQNGIPIADVGIALLPSATWTTFSLPFNLP
                     SMPDSVAITFGSDVHNPAQPSEVGDVLYVDEVLFLSQALPTATSVSWKNADAVTVYPN
                     PADDQVNIRLDLRSDAPAVMRVTDISGRIICVEQVSSAGTTVFPLDLSSQAPGVYFVT
                     LEQDGAKVVRKFVKN"
     gene            3950..5515
                     /locus_tag="VFU15_13805"
     CDS             3950..5515
                     /locus_tag="VFU15_13805"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEU4718912.1"
                     /translation="MKIVSPRLITFLASTALIGLCVIQYEWISGAVQQKHEHFGQDVR
                     EALMQVQRKYNRQQAEAGARLRRQLSYHRQSYVNNTGNPGNVSVFEEFVSDSNGQMNR
                     STHTHTYSGSKSETPLNTGVTPNSIISHSMRPSIFPQQDDYYSTKSGAFHNLFDEIIH
                     INIYDNYSDYVDTAVVDSLLKNELMSRGINTHYVWSVFNSPVCVFNCNQPSGDPYRDS
                     LLASHYRVSLTPDNIFTQPHMLAIYFPNERGFILREIWLMLALSVLFILFIILLFYYS
                     ITTIFRQKKLSEVKNDFISNMTHELKTPISTISLACEVLGDEGIEKTRERTDRYVGMI
                     RDENKRLGILVENVLQTAILDKGNFKLKPVDVNIHELIEQAIGNIRLAVEKKGGEISA
                     GLDATLPVISADKTHIQNVIYNLMDNAVKYSGENPVVRVSTKDAGSDIEICVQDNGIG
                     ISRENQKKIFDKLYRVPTGNVHDVKGFGLGLSYVKAIVEKHGGQVWVESEPGKGSRFF
                     VRIPRVPNEKPIL"
     gene            5512..6210
                     /locus_tag="VFU15_13810"
     CDS             5512..6210
                     /locus_tag="VFU15_13810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013927938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU4718913.1"
                     /translation="MTSETKTRVLLAEDDPNLGRLLQEYLDAKGYETVLATNGKEGFE
                     NFKKGKYDICLLDVMMPVKDGFTLAKEIRANDRDVPIVFLTAKSMKEDAIEGFNAGAD
                     DYITKPFSMEELLLRIKAILRRTKGVPLNMDQAQFTIGEYTFDYTRQMLERGQQGQKL
                     TSKEADLLRMLALHKNEVLDRSFALNTIWGDDSYFNSRSMDVYIAKLRKYLREDPTVE
                     IMNIHGRGFRLIAK"
     gene            complement(6291..7391)
                     /locus_tag="VFU15_13815"
     CDS             complement(6291..7391)
                     /locus_tag="VFU15_13815"
                     /inference="COORDINATES: protein motif:HMM:NF039534.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEU4718914.1"
                     /translation="MKIKALFAGLLLACVAHAQITLSITSVTPSCANPCNGSVTASAS
                     GGVPPYTYAISPTSATSLTGTFNNLCAGTYTVGVADANSAFATTTVTIGTISAPAITG
                     VVTSPVNPPFNYQAQVSYTGGQPPYYIYWVAMPSQSQIRIDTVMSMSDTITHLTPGDY
                     GVLINDSLNAAAGCIPAVTSPYPFSICDQSIGGGIISVTPNDTVCPGDQVTVTYSALL
                     FGPVNIIAQVFYSDNPNCDPSSSNGTFTCAPTQTTTFYGSWMYATGCAPIQYTPLTVT
                     VIPCTTGMQEAQGEDGISLFPNPGNGSFTVKGNSPDAMTVTIFDQSGRSCFSAVAHSG
                     DEIHAGLSAGIYFCTITSGDKTTTQKLVITGK"
BASE COUNT         1961 a         1820 c         2052 g         1773 t
ORIGIN      
        1 ttatttttct gtatcaagat gccaacactt tgattaatcg accttatctt attgttgact
       61 ggggggatat gacaacagat acgtgtaatg caggaacttc ggtcgcaatt actcccaatg
      121 tcctcaaaac cacttattcg atgccagtcc actcctttcc tcatagtggg gggtatgacg
      181 ttaatgttgc agatactttt ttagttccaa acattgaaaa cgtggcgtct ataaataatt
      241 atctctatat ccacacgtat tatgttgtaa gtccaaccct ttctaataac actccctatt
      301 tcataaacga tcaaaccgtc attacaaata gcaatggcac atactatcat tctgcgtctg
      361 ccgtagattc aaatggagat agtttagctt attatttgca gtatccttgg ggtgtctcag
      421 gatatgtacc acctaatgca acgatagacc cttatactgg tcttgtaact ttttttccgt
      481 attcaacggg attataccta atacctattg gggttgaaga atacagggat actggtactg
      541 gtgttcaatt aatttccatt actgagcggg agatgataat agaggttaat tcattggcat
      601 ctgtttcaga aaacgatttt gtacctcata ctttaactgt ttttcccaat cctgcaaaaa
      661 cctccgcgac ttttcagtta agcggacaaa tccaaatgtt tgaattaata ataacagatc
      721 aacttggccg agaagtttgg aggggtaata gtgattcgga cagacttcaa ttatcaacag
      781 aaaatttttt accgggaatg tacttttgtc gcatcgaaga acagggcgaa ataacggcaa
      841 gcggaaaact aataattgaa taaaaatggt agagcaagtt ttagaaatag tcaaacgcta
      901 cgaaataact gttgagggct tgggctataa agtctttgct aagattacaa aggagtatgg
      961 taaggaacgt tacgaatggt cggtaagtca ttactacatt ccgaatgcag gtatgggtat
     1021 ctatatgccc tccgcgaccg tgttcaatac atttgaggaa tgtgaacaca gattatttta
     1081 ttacgtcgat aaattcaccg cgaagtatgg aatacaggaa gaccccactt tcattaagta
     1141 atggataaag aaaaattaat tgagcttgca aaagaacggc tgcaaactct caataagata
     1201 cataaccttt tagctattgg tactttggta ctatgcggtc ttcctgtgat tgctaccgta
     1261 attattattt ggataagtga tagcaaaacg ttgttatacc cttgttctgt gttcgtagta
     1321 attgggttgc ttatttactt tttcattgaa agagcaaagc gaaaagcact tacaggagca
     1381 gcaaaatttc gttatgaact tgatcttttt atgaaaggcg ggagaggtta cgagggaggg
     1441 gatagcatta aaaagtttca agacttttta acgaatgagc ggtaagaatt gcgcggtttt
     1501 ttttgcgctg aaaaagaaac taaaagcata ctaacggaag ccaaaagcaa gacggggaaa
     1561 ggggctaaaa tccccttacg gcggggctgc gagtaaaccg ttaggccggg gagttatgaa
     1621 caggttctaa aacctcattt tcagctactt acgtggctta ttttacgaat atacgaataa
     1681 cggcaggcag gtatgtgtcc cgggtcacac aggaaataac aggaacaggg aagatgcggg
     1741 aggtgaaaga aatgttgtaa atttcttttg aagaacattc cgtaaatcgc cccgatgaac
     1801 actgtttttc cttcgcagtt cccgcacgtt ctcacgcagc ggctcgcact cggaaagata
     1861 accgggagcg atgcgcatgc cctgtatgat ctgtacagcg acgaacgcgt gatgaaattc
     1921 cacggccggc cggtgttagg tgcggcagag gaaacggaga agatcatttt cggatggaga
     1981 cggctcttcg ccacggaaaa gggaatccgc tgggggatcc gttttcgcgg cggggaaaag
     2041 atcatcggca cgatcggtgt ccggtgttct gcagagcacc gtcgtgcaga gacagggtac
     2101 gaacttgctc ccgcgctgtg gaaccagggg atcatgaccg aagcgctcgg tgctgtgacg
     2161 gactatgtgt tcacgcaaat gaatttcgag acgatagaag cgaacatagc gccgggcaac
     2221 acagcgtcgc agcgcgtgct ggaaaaagcg ggattccgcc aggaagctca tttccgttcg
     2281 aactattttt atgaaggctg gtgggattca gttgtctttt cgctgagcag ggaaacgcgc
     2341 aggtgaaaat gaaaaagccg ccccgggtgc ggaacggctt ttctgaataa taaactgcgt
     2401 tcagttcttc acaaatttcc ggacgacttt ggctccgtcc tgttcaagcg ttacaaaata
     2461 aacgccgggc gcctgcgaag aaagatcgag cgggaacacc gttgttccgg ccgagctgac
     2521 ctgctctacg caaataatgc ggccggaaat atccgtcacg cgcatcaccg cgggagcatc
     2581 gcttcgcagg tcgagccgga tgttgacctg gtcgtccgca ggattgggat ataccgttac
     2641 cgcatccgca tttttccacg acacggaagt tgccgtcggc agcgcctgcg aaaggaacag
     2701 cacttcatcc acgtaaagca cgtcgccgac ttccgaaggc tgggcaggat tgtgcacatc
     2761 agaaccgaaa gtgatggcga cggaatccgg catcgacgga agattgaacg gaagcgaaaa
     2821 tgttgtccac gttgcgctcg gcagcaatgc gatgcccaca tcggctatcg gtatcccgtt
     2881 ctgggaaaaa tgcgcgacgc cgatcgcggt gtcgagaccg ggcgagagga atttgtatgc
     2941 gccgacaagc gtgtcgatct gcagcgaata cggaaacccg ccgtccggga gcgcattggg
     3001 cacaggcctt ccgttggtga catagctcgg cgttgcgttg gactggctgt cgaaatacgt
     3061 tcccagcttc acggcgtact ggccggtgta atggtctgtc gtctgcatgg cggaatcgcc
     3121 gaacatcagc catgagttcg gtgtaaccgt cgtaaagctt gtccacagct cgaaatcgcc
     3181 gttcatcatc gcgggttgcg ttgcgacgcc ggtgaaggaa aggttgtcga gctggagcat
     3241 gcttccggga ataccattga acacaagcgc attgctcgaa gccgcagcga tgatcacgct
     3301 gtcgggaacg accaccgggg gcagcgagac gggcagcgag aacattgtgt aagtgctggc
     3361 cgtgcccgtg atcttctgta tcccgccgcc gataacggac ccgttcagct tgaaaacggc
     3421 gagtacaaga gcggtatcgc cggccggaac attgcatttg taataaccgg taagcgcggt
     3481 aggacgttgt gcatacggta ttccgcccga actgttcgag ggatcaccat tggagaaaaa
     3541 accgaacacc gtatcctggc cgttgctgac cgtctgcagg ttgatcgcgt atgtgttcag
     3601 ctgtgcgtcg gttgtgcgcg ttacgttcgg agccgggtaa cccagctggt gcacgttcgc
     3661 attggacgtg acgtaatact ggggctcata gtaggtgttc gatatccagc tttcaaaacc
     3721 accgttggga acagaaacct gtgcggtagc ggcggcggaa aacaaaagca gtaaagcgta
     3781 aagtaatttt cgtttcatga agtttgtttt tgtttgttaa tcaaatttaa ataaaacaac
     3841 cggatgcttc cgggacgcgt tacacagtga aggagggacg gattttccgt gcagttaaag
     3901 aatgttaacc gaaaaattgc agcgagggtg gttttctaaa tttgtaagaa tgaaaatcgt
     3961 tagtccgcgg ctgatcactt ttctcgcgag cacggctctt atcggcctgt gcgtgatcca
     4021 gtatgagtgg atcagcgggg ctgtgcagca gaagcatgaa catttcgggc aggacgtgcg
     4081 ggaagcgctc atgcaggtgc agcgcaaata caaccggcag caggccgaag cgggcgcgcg
     4141 tcttcgccgc cagctcagct atcaccgcca gagctatgtg aacaatacgg gaaatcccgg
     4201 gaacgtgagc gtgttcgagg agtttgtttc cgattcgaac gggcagatga accgcagcac
     4261 gcatacgcat acctacagcg gatcgaaaag cgagacgccg ctcaatacgg gcgtcacccc
     4321 gaacagcatc atttcgcatt ccatgcgtcc gtcgatcttc ccgcagcagg acgattatta
     4381 ttccacgaaa tcgggtgcgt tccacaacct gttcgacgag atcatccaca tcaatattta
     4441 cgacaactac agtgattacg tggatacggc ggtggtcgat tcactgctga agaacgagct
     4501 gatgagccgg ggcatcaaca cgcattatgt ctggtcggtg ttcaattcgc cggtgtgcgt
     4561 gttcaactgc aaccagccct ccggcgatcc atacagggac agcctgctcg ccagccatta
     4621 ccgcgtttca ctgacgccgg ataatatttt cacccagccg cacatgcttg ccatttattt
     4681 tccgaacgag cgcgggttca ttctccgcga gatctggctg atgcttgcgc tttccgtgct
     4741 cttcatcctt ttcatcatcc tgctgttcta ttattccatc acaaccatct tccgccagaa
     4801 aaaactttcg gaagtgaaga acgatttcat cagcaacatg acgcacgagc tgaaaacgcc
     4861 gatctccacg atctcgctgg catgcgaagt gctgggtgac gaaggaattg aaaaaacgcg
     4921 ggaacgcact gaccgttacg tgggcatgat ccgcgacgag aacaagcgcc tcggcatcct
     4981 cgtcgaaaat gtcctgcaga cggccattct cgataaggga aatttcaagc tcaagccggt
     5041 ggacgtgaat atccacgaac tcatcgagca ggcgatcggg aatatccgcc ttgccgtgga
     5101 gaaaaaaggc ggcgagatat ccgccggcct tgacgcgacg ctgccggtca tctccgctga
     5161 caaaacgcac atccagaacg ttatttacaa cctgatggac aacgcggtga aatattccgg
     5221 cgaaaatccc gttgtacgtg tctcgaccaa agatgccggt tccgacatcg agatctgtgt
     5281 gcaggacaat ggtatcggca tcagccgcga gaaccagaaa aagatattcg ataaattata
     5341 ccgtgttccc accgggaacg ttcatgatgt gaaaggtttc ggcctcgggc tcagctacgt
     5401 gaaagcgatc gtcgagaaac acggcggaca ggtatgggtg gaaagcgaac ctggcaaagg
     5461 aagccgtttc tttgtacgaa ttccaagagt tcccaatgaa aaaccgattt tatgacctca
     5521 gaaaccaaaa cccgcgtgtt gcttgctgaa gatgatccga acctcggccg tcttctccag
     5581 gaatacctcg acgcaaaagg gtatgaaact gttcttgcca ccaatggaaa agaaggcttc
     5641 gaaaatttca aaaaaggaaa atacgacatc tgcctgctgg atgtgatgat gcccgtgaag
     5701 gacggcttca cgctggccaa agagatccgt gcgaacgaca gggatgttcc catcgttttc
     5761 ctcactgcaa aatccatgaa ggaagatgcg atcgaaggat tcaacgcggg agccgatgat
     5821 tacatcacca agccgttctc gatggaggag ctgctcctcc gcatcaaggc gattctccgc
     5881 cgcacgaaag gtgttccgct gaacatggac caggcgcagt tcacgatcgg tgaatacaca
     5941 ttcgattaca cgcggcagat gctcgagcgc gggcagcagg gacagaaact cacgtcgaaa
     6001 gaggccgacc tgctgcgcat gctcgcgctg cacaagaacg aggtgctcga ccgttccttc
     6061 gcgctgaaca cgatctgggg ggacgacagc tatttcaatt cacgcagcat ggatgtgtat
     6121 atcgcgaagc tgcgcaaata cctgcgcgaa gatcccactg tcgagatcat gaacatccac
     6181 ggaagaggat tccggctcat cgccaaatga tctttctcaa aacacaataa aaaaatccgg
     6241 aagatgctct cccggatttt tttatttcag atgaatgcgg accggaaatt ttatttcccg
     6301 gtgatcacca gcttctgcgt tgtggtttta tcaccggaag tgatcgtgca gaaatagatg
     6361 cctgctgaaa gtcccgcatg aatttcatcg ccgctgtgcg caactgcgct gaagcagctt
     6421 cttcccgact ggtcgaagat cgtgaccgtc atcgcgtccg ggctgtttcc ttttaccgtg
     6481 aagcttccgt tgcccggatt ggggaaaagc gaaatgccgt cctcgccctg cgcttcctgc
     6541 atgccggtgg tacacgggat cactgtaacc gtgagcggcg tgtactggat gggcgcacaa
     6601 cctgtcgcat acatccacga tccgtagaac gtggtggtct gcgtgggtgc gcaggtaaag
     6661 gtcccgttcg aggaagaagg atcgcagttc gggttgtcgg aatagaatac ctgcgcgata
     6721 atattgaccg gtccgaaaag aagcgcggaa taagtcaccg tgacctggtc accgggacaa
     6781 accgtgtcat tcggagtaac ggaaattatt cccccgccta tcgactggtc gcagatggag
     6841 aatggatacg gcgacgtgac cgcgggaatg cagccggccg ccgcgttcag cgaatcgttg
     6901 atgagaacgc cgtaatctcc gggcgtaaga tgcgtgatcg tgtcgctcat cgacatcaca
     6961 gtgtcgatcc ggatctggct ttgcgaaggc atcgctaccc agtaaatgta atacgggggc
     7021 tgtccgcctg tatagcttac ctgcgcctgg taattgaacg gcggattgac cggtgacgtg
     7081 acgacgcccg tgatggccgg tgcggagatc gttcctatgg ttaccgttgt agtggcaaac
     7141 gcagaattcg catcagcgac acccaccgtg taggtgccgg cgcaaaggtt gttgaaggtt
     7201 cctgtcaggc ttgtcgccga ggtgggcgag atcgcgtacg tgtagggcgg aacgccgccg
     7261 gaagccgaag cggtcacgct cccgttgcac ggattggcgc agctgggtgt aacgctggtg
     7321 atgctgagag tgatctgtgc gtgagccacg caggcgagca gcaggcccgc aaaaagcgct
     7381 ttgattttca taccggaaaa agtgtttgag tggttaccgc tatgaaaata gcaaattttc
     7441 ccggatcaga acttcccgga gatgccgacg ccaagcacct gccggaactg aaggcggggg
     7501 ccggttgcat cgatcacccc gtcgtggttg gtgtcgacgg caatgggaat atcgttgtcg
     7561 taaatggcct gcatgccgaa gctggccgcg agaaatttgt tgatct
//
LOCUS       DASTSF010000478         5393 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_527725,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000478 DASTSF010000000
VERSION     DASTSF010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5393
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_527725"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..494)
                     /locus_tag="VFU15_13820"
     CDS             complement(<1..494)
                     /locus_tag="VFU15_13820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718915.1"
                     /translation="MKYRFLIFLLAGAPGCVFAQSGMDNAFSSDIMTNQVMEAKILDP
                     AVFIASAGTTNVVQSNAQASAYWGKYVNSNPSNAGAWMNYYRSVRFCAESQNTEAASR
                     PRLDSIDNQMQVRIPGTFEQLLVHYWNGFHDISRYASLEKAWKMRPSDPLVLRQMAGY
                     NFITG"
     gene            608..3646
                     /locus_tag="VFU15_13825"
     CDS             608..3646
                     /locus_tag="VFU15_13825"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HEU4718916.1"
                     /translation="MKSLPLLPVFLLLSALSLRGQQYNFRNYSVADGLAQSQVYAMTE
                     DARGYLWLGTQGGGLSRFDGQQFRTYTEDDSLCNGFVRCLTTDKNGMLWIGTNEGVCC
                     FDGQRFRKINFTCGGGAINGLTFSSDGRLWVCTDDSGLVVLKDEKVNFRLTTKRNLPS
                     NRVFCAFENTDGTMWIGTEQGLEKISGSYRKPFTVKDGLPNAGIRGIINDGKGNLWMA
                     TYGGGLVYFNPDSINLSFRKFTTENGLTNNTTHCIMKDKSGRIWVGTASGVTRISDVV
                     KQFSENEGLCSNVVMSVLEDSWGNVWFGTSGGGACVLDGERFIHFNEKSGDMGTWVYA
                     LHCDRKGNMWFATSKGGVTEYDGMYYTNYYEGAGFTSAKVRAFGEDTSGTLWFGTVGD
                     GAYCLKKGTFHHYRHSDGLGSNFVYAFLTDSMNREWMGTLGGAISVFNPADESVTRVG
                     AKQGVTNDRIYDLAADESGNIFACSNGSGVYEMEYDSTGIRVKKQFTKKDGLPGNNVR
                     SATCDKFGYMWFGTADGGIARFDGVSFKTVSKKDGLASNNIYLLIADRQGSLWVGTEK
                     GLDKLSYDARGTVFSIKHYGKGEGLYGIETSQNAACLDTGNGVWFGTISGASLYQPEG
                     DYITNEPPKVHLTGIRLFFDQIENTPYGIFRKDADHWFPVPDSLVLPYSENSLRFEFT
                     GIDLRNPDGVRFRWKLDDFDKTWTPENTERQAIYSNLPPGDYVFELQARNSDGFWSDT
                     QKFSFHITPPYYATWPFRLGASGIVLAVILLLFNWRIRIVKRRNRAQLEKITLEKHVL
                     ELEQKALRLQMNPHFIFNALQSIQGFIARNDSAEARRYLAKFGKLMRHTLDNSRQTYT
                     SIAQEAESLEHYLGLEALCHGERFTYSIAIAADIDPDATFIPVMLIQPFVENAVKHGV
                     SHLQERKGNISVRFRKEDHSIVCEVEDNGVGRNKSAELEDGFRKDHRSAALQITTERL
                     GQQMENEKRSGELQIIDLEDENGNSSGTKVVIQIMNVVFE"
     gene            3707..4516
                     /locus_tag="VFU15_13830"
     CDS             3707..4516
                     /locus_tag="VFU15_13830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012780357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M90 family metallopeptidase"
                     /protein_id="HEU4718917.1"
                     /translation="MALFILALASIAIMVIARSSFLKQRRIREKLDEEFPAAWRSVLQ
                     HKVLFYSRLGQADKNLFEKRVQLFLATKNIEGVDTEIDDPIRLMVACSAIIPTFAFPD
                     YNYPNVRSVLIYPNSFDEHFQTQRFDGHREIISGMVGDRFMNGTVILSKPDLVSAFDG
                     QLHKSNVGIHEFVHLLDKEDGAIDGIPEALMDHRYAGPWLHEIKNEMRKIEAGRSDIS
                     PYSLTNNAEFLAVVSEYFFDNPGKFRRKHPELYKILSSVFRTDKNNPGTQE"
     gene            complement(4558..>5393)
                     /locus_tag="VFU15_13835"
     CDS             complement(4558..>5393)
                     /locus_tag="VFU15_13835"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEU4718918.1"
                     /translation="DDLYREKLFSEFWNVDVEKLGRDLLDAGSGATYAHLCRIVMLNA
                     SPEKKNARVIVDKNPMYYYFLREIREIFPEAKFIHLVRDYRGNIASHKKVFLMKKVRD
                     IAWKWLWVNREIEALKKNMPGSFFTLRYEDVVANPGEEMKKICGFLGVDYSAEMISDH
                     TEKIYPAFRNKKDAPGFGGIHASLFGKMSPEIPDKWKKELSPGEILAAEKIDGDFGKK
                     MYGYEPTIPPGENHSAPAGIFVRMKFFMVRTFTRIFVRNRLTSPAMRLLMRTLIRIYK
                     S"
BASE COUNT         1320 a         1438 c         1426 g         1209 t
ORIGIN      
        1 ccggtgatga aattataacc cgccatctgc cgcagtacaa gcggatccga cgggcgcatc
       61 ttccacgcct tttccagcga agcgtaacgg gaaatgtcat gaaagccgtt ccagtaatgc
      121 acgagcagct gctcgaacgt cccgggaatt cttacctgca tctggttatc gatcgaatca
      181 agacgcggac gggatgcagc ctccgtattc tgggattccg cgcagaaacg gacggaacgg
      241 taatagttca tccacgctcc ggcgttcgaa ggattgctgt tcacgtattt gccccaatag
      301 gcggaagcct gcgcgttcga ctgcacgacg ttcgtcgtgc cggcgctcgc gataaaaaca
      361 gccggatcga ggatcttcgc ctccatcacc tggttcgtca tgatgtccga gctgaacgca
      421 ttgtccatgc cggactgcgc gaaaacacat cccggtgcgc cggcgagtaa gaaaatgaga
      481 aaacggtatt tcatgcctgc cttgctattc aagttacgaa atgggatcat accttcctcc
      541 tgtacactga aaatcgcgca aagttcatgc gaaaaacatc tttttcccgt taacttggag
      601 caccggaatg aaatcccttc ccctgctgcc ggttttcctt cttctttctg cgctttccct
      661 gcgtggacag caatacaatt tccgtaatta ttccgtagcc gacgggctcg cccagtcgca
      721 ggtgtatgcc atgacggagg acgcgcgcgg ttatctctgg ctcggaacgc agggcggcgg
      781 gctcagccgt ttcgacgggc agcaattccg cacctacacc gaggacgaca gcctgtgcaa
      841 cggttttgtg cgctgcctga ccacagacaa gaacggcatg ctctggatcg gcacgaacga
      901 aggcgtgtgc tgcttcgacg ggcagcgctt ccggaaaata aatttcacct gtggcggcgg
      961 ggcgatcaac gggctcactt tttccagtga cggaaggttg tgggtctgca ccgacgacag
     1021 cgggctcgtt gtgctgaaag atgaaaaagt aaatttccgc ctcacgacga aacgcaatct
     1081 tccttccaac cgcgttttct gcgcatttga aaacacggac ggaaccatgt ggatcggtac
     1141 ggaacaggga ctcgaaaaaa tttccgggag ctaccggaaa ccgttcactg taaaagacgg
     1201 gttgcccaac gcgggaatac gcggcattat caacgacgga aaaggaaacc tgtggatggc
     1261 cacgtatggc ggcgggctcg tttatttcaa tcccgacagc atcaatcttt ctttccggaa
     1321 attcacaacg gagaacggtc tcacgaacaa caccacgcat tgcatcatga aagataaaag
     1381 cgggcggata tgggtcggca ccgcttccgg cgtaacaagg atcagcgatg tggtgaaaca
     1441 attttcagag aacgaaggtt tgtgcagcaa tgtcgtcatg tcggtgctgg aagattcctg
     1501 gggaaacgtc tggttcggga catcgggcgg cggcgcctgc gtgctcgacg gggaacgctt
     1561 cattcatttc aacgaaaaaa gcggcgacat gggaacgtgg gtttacgcgc ttcactgcga
     1621 caggaaaggg aatatgtggt tcgctacgtc gaaaggcggc gtcaccgaat acgacggcat
     1681 gtactacacg aactattacg aaggcgccgg gtttacttct gcaaaagtcc gcgcgttcgg
     1741 tgaagacacc agcggcacac tctggttcgg cacggttggc gacggcgcgt attgcctgaa
     1801 aaaaggcacg ttccaccatt accgtcacag tgacgggctc ggtagcaatt tcgtttatgc
     1861 gttcctcacc gattcgatga accgggaatg gatgggcacg ctcggcggcg ccatttcggt
     1921 tttcaatcct gcggatgaat cagtcacacg cgtgggcgcg aagcagggtg tgacgaacga
     1981 caggatctat gatctcgcgg ccgacgaaag cgggaatatt ttcgcgtgca gcaacggaag
     2041 cggcgtgtat gaaatggaat acgacagcac gggcattcgc gtgaaaaaac agttcacgaa
     2101 gaaagacggg ctgccgggca acaacgtgcg ttccgccacg tgcgacaaat tcggttacat
     2161 gtggttcggc actgccgacg gcggcatcgc gcgtttcgac ggcgtgagtt tcaaaaccgt
     2221 ttcgaaaaaa gacggcctcg cgtcgaacaa catttacctg ctcatcgccg accgccaggg
     2281 ttcgctctgg gtgggaacgg aaaaaggtct cgacaagctg agctacgacg ccaggggaac
     2341 tgttttttcc atcaagcact atggcaaagg cgaaggattg tacggaattg aaaccagcca
     2401 gaacgccgcc tgtctcgata ccgggaacgg cgtctggttc ggaacgattt ccggtgcgag
     2461 tctttaccag cccgaaggcg attacatcac caacgaaccg ccgaaagtgc atctcaccgg
     2521 catacgtctt ttcttcgatc agatcgaaaa cacaccttac ggcattttca ggaaagacgc
     2581 cgatcactgg ttccctgttc ccgattcgct tgtcctgccc tattctgaaa attcgctccg
     2641 cttcgaattc accggcatcg atctccgcaa tcccgatggc gtccgcttcc ggtggaagct
     2701 cgatgatttc gataaaacgt ggacaccgga aaacaccgaa cggcaggcga tctattccaa
     2761 ccttccgccg ggtgattatg tcttcgagct gcaggcccgc aactccgacg ggttctggag
     2821 cgacacgcag aaattttcat tccacattac gcctccgtat tacgccacgt ggccgttccg
     2881 cctcggtgcg tcgggcattg tgctcgcggt catcctcctg cttttcaact ggcgcatccg
     2941 gatcgtcaag cggagaaacc gcgcacagct ggaaaagatc acgctggaaa agcacgtgct
     3001 cgagctcgag cagaaagcgt tgcgcctgca gatgaacccg cattttatct tcaatgcgct
     3061 gcagtcgata cagggtttca tcgctcggaa cgattccgcg gaagcacgca ggtacctggc
     3121 gaagttcggg aagctcatgc ggcacacgct cgacaattcg cggcagacgt acacgagcat
     3181 tgcgcaggaa gcggaatcgc tggaacatta cctcgggctc gaagctttgt gccacggcga
     3241 acgttttacc tactccattg cgatcgccgc agacatcgat cctgacgcga cgttcattcc
     3301 cgtcatgctg atccagcctt tcgtggaaaa tgcggtgaaa catggcgtca gtcacctgca
     3361 ggaacggaaa ggaaatattt ccgtacgctt ccggaaagaa gatcattcga tcgtctgcga
     3421 agtggaagac aacggcgtgg gaagaaataa atctgctgag ctggaagacg gcttcaggaa
     3481 agatcaccgc tcggctgcgc tgcagatcac gacggaacgt ctcgggcagc aaatggagaa
     3541 cgaaaaacgt tccggtgaat tgcagatcat cgacctcgaa gatgaaaacg ggaattcttc
     3601 cggaacgaaa gtggtgatac agatcatgaa cgtggtcttc gaataaaaac agaattcgca
     3661 atcggtaacc cggctttctg cttacctttt tctgacaggc aactcaatgg ctctatttat
     3721 cctggccctg gcatcgatcg caatcatggt cattgcacgg tcgtcgtttc ttaaacaaag
     3781 acgtatccgg gaaaagctgg acgaggaatt tcctgcagca tggcgctcag tgcttcagca
     3841 taaagtcctg ttttattcga ggctcggcca ggctgacaaa aatctttttg agaaacgcgt
     3901 gcagcttttc cttgcgacaa aaaacataga aggagtggat acggagatcg acgacccgat
     3961 ccggctgatg gttgcttgca gcgccatcat tcccacgttc gctttccccg attacaatta
     4021 tccaaacgtg cggtccgtac tgatctatcc caacagcttc gacgaacatt ttcagacaca
     4081 gcgcttcgac ggccatcgcg aaattatttc aggaatggtc ggcgaccgtt ttatgaatgg
     4141 cacggttatt ttgtcaaagc ccgacctcgt ctccgccttt gacgggcagt tgcataaatc
     4201 caatgtcggg atccacgagt ttgttcatct gctggacaag gaagacggag cgattgacgg
     4261 tattccggaa gcattgatgg accaccgtta tgcaggaccc tggctgcatg aaattaaaaa
     4321 tgaaatgcgg aaaattgaag cgggacgatc ggatattagt ccatactcgc tgacaaacaa
     4381 cgcagaattc ctggccgtag tcagtgaata ctttttcgac aaccccggaa aatttcgtcg
     4441 aaagcatcca gaactctaca aaatactttc ctctgttttc agaacggata aaaataatcc
     4501 ggggacgcag gaatgatcgt cgtttgaaaa aataccggga ggaaggaaaa caacacttta
     4561 cgatttgtag atgcggatca gcgttcgcat caacaggcgc atggccgggg aagtaaggcg
     4621 gttgcggaca aaaatacgtg tgaaggtgcg caccatgaaa aatttcatcc gcacaaaaat
     4681 tcccgcagga gcggaatggt tttcacccgg cgggatcgtc ggttcatacc cgtacatttt
     4741 ttttccgaaa tccccgtcga tcttttccgc tgccagtatc tcacccggac taagctcctt
     4801 tttccatttg tcaggtatct ccggcgacat tttcccgaac aggctcgcat gaattccgcc
     4861 gaaaccgggc gcatcctttt tattccggaa cgcgggataa attttttccg tgtgatcgct
     4921 gatcatctcc gcggaataat ccacgccgag gaaaccgcat atctttttca tctcctcccc
     4981 gggattggcc accacatctt cgtacctcag cgtaaaaaaa gagccgggca tgttcttttt
     5041 cagcgcttcg atctcgcggt tcacccacaa ccatttccat gcgatgtcgc ggaccttttt
     5101 cataagaaaa actttcttgt ggctggcgat gttgccgcgg taatcgcgca ccaggtgaat
     5161 gaacttcgcc tccgggaaaa tttccctgat ctcgcgcagg aaataataat acatggggtt
     5221 cttgtcgacg atgacgcgcg cgttcttttt ttccggcgat gcgttcagca tcacgatgcg
     5281 gcacaggtgt gcgtaggttg cgccgctgcc tgcatccagc aggtcccgcc cgagcttttc
     5341 cacgtcgacg ttccagaatt cgctgaagag tttttcccgg taaagatcat cca
//
LOCUS       DASTSF010000479         4580 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_545118,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000479 DASTSF010000000
VERSION     DASTSF010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4580
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_545118"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..299)
                     /locus_tag="VFU15_13840"
     CDS             complement(<1..299)
                     /locus_tag="VFU15_13840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718919.1"
                     /translation="MSFSRKFSAFATAGMLLLFVASWKSAEEERIIVDGIDLRHLLVS
                     DTALGGPVNRFILEGKRKTALTENEFFIDTNRLGMGYSSFLPQIRKNDSLRNFFTG"
     gene            complement(310..1254)
                     /locus_tag="VFU15_13845"
     CDS             complement(310..1254)
                     /locus_tag="VFU15_13845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718920.1"
                     /translation="MENQPQPAKEKEKDNRRLHAIYLTIIFLLAGLCVFLSMQYRELK
                     TVVATHEIKIEQVVKERDDVKSDLEDLRAQYDQLQTNDKTISAELEAKKAYIDSLLVE
                     ADKHKNDSYVIAKLKKETMTLRKIMQGYIHTIDSLNQLNDKLVAEKNEVLTQLDDQKS
                     KTQQVTDEKDKLKGRIDKAALLSTLNVKAVGVKTSRGGKKDSETSKASKVDKIRVTFD
                     VADNDLTTPGDKDIYIRIITPDAQEMSESKDASHQFSFGNTTSYFAGKKTIDYENQPM
                     SVLISCPKPKDTDLVPGKYLIEIYADKVMIGSTGMILE"
     gene            1323..2033
                     /locus_tag="VFU15_13850"
     CDS             1323..2033
                     /locus_tag="VFU15_13850"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HEU4718921.1"
                     /translation="MASATLKQLGADFISLFFPVTCAACNGELVRGEEAICTSCLSFL
                     PRTSDENDPRENPVARVFWGRVPLQCAASAFLFSKGGSVQELIHNLKYNGRKDAGIAA
                     GKFFGNDLKKLTPFNSVDAVVPVPLHSEKFRKRGYNQAACFGQGIAHALGVPLLGRAV
                     LRMTATETQTRKSRTERWSNVEDVFRANPEINVSGKHILLVDDVITTGATIEACASAL
                     LACEGTSVSVVSLASARE"
     gene            2138..2635
                     /locus_tag="VFU15_13855"
     CDS             2138..2635
                     /locus_tag="VFU15_13855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEU4718922.1"
                     /translation="MAESLVISGTTKEERYRSLLPQLVALTDGENDLIANLSNITGAL
                     RQAFGFFWTGVYFVKGDQLVLGPFQGPVACTRISRGRGVCGTCWEKKQAILVPDVDQF
                     PGHIACSSDSKSEIVVPVFGDDGEVILVLDIDSDKLNDFDATDRQYLEEVAGLISVLH
                     NRRDA"
     gene            2628..4292
                     /locus_tag="VFU15_13860"
     CDS             2628..4292
                     /locus_tag="VFU15_13860"
                     /inference="COORDINATES: protein motif:HMM:NF024603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEU4718923.1"
                     /translation="MRKLLLISVPVLLLASCAQVISPGGGPKDITPPVVVRYLPDSAA
                     TNFTGKKIDIRFNEYVRLTDLNNQLIISPPLKNMPDVEVKKKDVVITFDDTLLPNTTY
                     TISFGKAIADITEGNVLDNFRYVFSTGPYIDSLEIKGKVINASTLDGEKGIYVMLYQQ
                     TDDSVPMKQRPYYFTKTKEDGSFLFTNLRSGKYKVFALDDKNSNYLFDNSEERVAFAD
                     APIDLHANIDSLKLKLFHEEPSSQKRLRAAQVCPGRVTFMYAFPVADPVITFLPALNS
                     GMVPYAEKSRNGDSLDVWFSNVTSDSLIFRITDGKTLVDSVMLPLTRPGVKSSHVRAG
                     SVDARKLQLSANIAAGQRLDYAKPLTVTTNNPIVKWDTSGIILVRGKDTLSTALKFSG
                     DSVPKQVSAGPIHPGGSMRTFVFTNTMEEDSTYLLFIKPGAMTDCFGQKNDTLKMVFR
                     VQSQDAYGNLHVKLTDLPAEHYIIQVLSDKGEVVRQSKVTGDDLHFDLLSPGNYRLKL
                     IDDENNNGKWDPGNYLLHRQPERVIFYKNSVRLRAGWDMDVEWIFK"
     gene            complement(4338..>4580)
                     /locus_tag="VFU15_13865"
     CDS             complement(4338..>4580)
                     /locus_tag="VFU15_13865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718924.1"
                     /translation="QHWKIGTTSGNHFNHEGHKAFHKAHEGKRGQSRFNIGKLERQAG
                     IILTTKDTKHFTKRTKEDEDNPGSTLENWNDKRESS"
BASE COUNT          998 a         1183 c         1257 g         1142 t
ORIGIN      
        1 ccggtgaaaa aattgcggag cgaatcgttt ttccggatct gcggcaggaa agaagaatag
       61 cccatgccga gccggttcgt atcaatgaag aattcatttt ccgtgagcgc tgttttgcgt
      121 tttccctcga ggatgaagcg attcacgggt ccgccaagcg ccgtatcgga aaccagcagg
      181 tggcggagat cgatcccgtc gacgatgatc cgttcctctt ccgcggattt ccacgaagcg
      241 acgaaaagca ggagcattcc cgcagttgca aaggcggaaa atttcctgct gaagctcatg
      301 ttgtgaggat tattcgagga tcattcccgt cgagccgatc atcactttgt cggcgtagat
      361 ctcgataagg tatttcccgg gaacgaggtc cgtgtctttc ggtttcgggc agctgatgag
      421 cacgctcatg ggctggttct cgtaatcgat ggtctttttg cctgcgaaat acgaggtcgt
      481 gttcccgaat gagaactggt gcgaagcatc tttgctttcc gacatttcct gcgcatccgg
      541 cgtgatgatg cggatgtaga tgtctttgtc gcccggcgtt gtaagatcgt tatccgccac
      601 atcaaatgtt acgcggatct tgtcgacctt gcttgccttg ctcgtttcgg aatctttttt
      661 tccgccgcgt gatgttttca caccgacggc tttcacatta agtgttgaga gcagcgctgc
      721 cttgtcgatg cggcctttga gcttgtcttt ttcgtcggtg acctgctgcg ttttgctttt
      781 ctggtcgtcg agctgcgtca gcacttcgtt cttttccgca acgagcttat cgttgagctg
      841 gttcagcgag tcgatcgtgt ggatatagcc ctgcatgatc ttgcggagcg tcatcgtttc
      901 ttttttcagc ttcgcgataa cgtaggaatc gttcttgtgc ttgtccgctt ccacgagcag
      961 cgaatcgata taggcttttt tcgcttccag ctccgcggaa atggtcttgt cgttcgtctg
     1021 cagctggtcg tactgcgcgc gcaggtcctc gagatcgctt ttcacgtcgt cgcgttcctt
     1081 caccacctgc tcgatcttta tttcgtgcgt ggcgacgact gttttcagtt cgcggtactg
     1141 catcgaaaga aaaacgcaga gacctgcgag caggaaaatg atcgtaaggt aaatggcgtg
     1201 gagacgccgg ttgtcttttt ccttttcttt cgcgggttgt ggctgatttt ccatagtcag
     1261 tgaaattgct gttggtgatg cagcaaaaaa tattccttta tttgccgcaa aattacattt
     1321 ttatggcctc agccacatta aaacagctcg gtgcggattt tatttccctg ttctttcccg
     1381 ttacctgcgc agcctgcaac ggtgagctgg tgcgcggcga agaggcgatc tgcacgtcct
     1441 gtctatcttt cctgccgagg acatccgacg agaacgatcc gcgggaaaac ccggtggcgc
     1501 gcgtgttctg ggggcgcgtc ccgctgcaat gcgcggcgtc ggctttcctg ttttccaagg
     1561 gtggaagcgt gcaggagctg atccataatc tcaaatacaa cggaagaaag gacgccggaa
     1621 ttgccgccgg aaagtttttt gggaatgatc tgaaaaaatt aacgcctttt aacagcgtgg
     1681 acgccgtagt gcccgttccg ctgcacagtg aaaaattccg caaaagagga tacaaccagg
     1741 ccgcctgttt cgggcaaggg atcgcacatg cgctgggtgt gccgctgctc ggccgtgccg
     1801 ttctgcgcat gaccgcgaca gaaacgcaga cacggaaatc acgcacggag cgatggagca
     1861 acgtggagga tgttttccgc gccaacccgg aaataaacgt aagcggaaaa cacatcctgc
     1921 ttgttgacga tgtcatcact accggtgcga cgatcgaggc gtgcgcatcg gcattgctgg
     1981 catgtgaagg aacgagcgtg agcgtcgttt ccctggcgag cgcgagggaa taaggcgttt
     2041 gccaaaaaat aacaagacga ggacgccggg cggttaacgg caaacttttt gcgcctgcaa
     2101 ggttctccaa aaatccgatc tttaccacac ttttcccatg gccgaatcgc ttgtcatttc
     2161 cggaacaacg aaagaagaac gctaccgttc cctgcttccg cagctggttg cgctgacgga
     2221 cggcgagaac gatctcatcg cgaacctttc caacatcacc ggcgcgctgc ggcaggcgtt
     2281 cggatttttc tggaccggcg tttatttcgt gaaaggggac cagcttgtgc tcggaccttt
     2341 ccagggacct gttgcgtgca cgcgcatcag cagggggcgc ggcgtatgcg gaacatgctg
     2401 ggagaaaaag caggcgatac ttgttcccga tgtcgaccag tttcccgggc acatcgcatg
     2461 cagctccgat tcgaagtcgg agatcgtcgt tcctgttttc ggtgatgacg gcgaagtcat
     2521 tctcgtgctc gacatcgaca gcgacaagct gaacgatttc gacgcgaccg accggcaata
     2581 cctcgaagaa gtggccggac ttatttccgt tttacataac cgccgggatg cgtaagctgc
     2641 tgctgatctc cgttcccgtt ctcctcctcg cgtcctgcgc gcaggtgatc tcgcccggcg
     2701 gaggtccgaa agacatcacg ccgcctgttg tagtgaggta cctgcccgac agcgccgcca
     2761 cgaacttcac ggggaaaaag atcgatatcc gtttcaacga atacgtccgc cttacggatc
     2821 tgaacaacca gctcatcatt tcgccgccgc tgaaaaatat gcccgacgtt gaggtgaaga
     2881 agaaagatgt cgtgatcacg ttcgacgata cgctgctgcc caatacgacg tacacgatct
     2941 ccttcggaaa agccattgct gacattacgg aagggaatgt gctcgataat ttccggtacg
     3001 ttttttccac cgggccgtac atcgattcgc ttgaaataaa agggaaggtc atcaatgcgt
     3061 cgacgctgga cggggagaag gggatttacg tgatgcttta ccagcagacg gatgattcgg
     3121 tcccgatgaa gcagcgtccg tattatttca cgaagacgaa agaggacggc tccttcctgt
     3181 tcaccaacct cagatcgggg aagtacaagg tctttgcgct cgacgataaa aattcaaatt
     3241 accttttcga caactccgaa gaacgcgtgg catttgcaga cgctcccatc gaccttcacg
     3301 cgaacatcga ttcgctgaag ctgaaattat ttcatgaaga gccgtcgtcg cagaaaagat
     3361 tgcgcgcggc gcaggtctgc ccgggaagag tgacattcat gtatgcgttc ccggtcgccg
     3421 acccggtgat cacgtttctt cctgcgctga acagcggcat ggtcccgtac gcagaaaaaa
     3481 gcaggaacgg cgattcgctc gacgtatggt tcagcaatgt caccagcgat tcgctgatct
     3541 tccgcataac ggatggaaaa acgctcgtcg attccgtcat gctgccgctg acgaggccgg
     3601 gtgtgaaatc atcgcacgtg cgtgcgggca gcgtggatgc gcgtaagctg cagctttccg
     3661 cgaacatcgc cgccgggcag cggctcgatt acgcaaaacc gctgacggtc accacgaaca
     3721 accccattgt gaaatgggac acgtcgggaa tcattctcgt gcgtggaaaa gatacgctga
     3781 gcaccgcgct gaaattctca ggcgattcag ttccgaagca ggtaagcgcg ggacccatcc
     3841 accccggcgg gagcatgcgt acgttcgtgt ttacgaatac aatggaagag gacagcacgt
     3901 acctgctttt catcaaaccg ggcgcaatga cggattgttt cgggcagaag aacgacacgc
     3961 tgaagatggt tttccgcgtg caaagccagg atgcgtacgg aaacctgcac gtgaagctga
     4021 cggatcttcc cgcggagcac tacataatcc aggtgctcag tgacaagggc gaagtggtgc
     4081 ggcaatcaaa agtgacgggc gacgacctgc atttcgatct tctttcacca gggaattaca
     4141 ggttgaagct gatcgacgac gagaacaata acgggaaatg ggatccggga aattatcttc
     4201 tgcacaggca gccggaacgt gtgatcttct acaaaaattc tgtgcgcctc cgcgcaggat
     4261 gggatatgga cgtggagtgg atatttaaat agtttccagg ttgctgtttt ccttcgtgcg
     4321 ctttgtactc ttcgtggtta agatgattcc cgcttgtcgt tccaattttc caatgtcgaa
     4381 cctggattgt cctcgtcttc cttcgtgcgc tttgtgaaat gctttgtgtc cttcgtggtt
     4441 aaaatgattc ccgcttgtcg ttccaatttt ccaatgttga acctggattg tcctcgtttt
     4501 ccttcgtgcg ctttgtgaaa tgctttgtgt ccttcgtggt taaaatgatt cccgcttgtc
     4561 gttccaattt tccaatgttg
//
LOCUS       DASTSF010000480         2295 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_213370,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000480 DASTSF010000000
VERSION     DASTSF010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2295
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213370"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="VFU15_13870"
     CDS             <1..200
                     /locus_tag="VFU15_13870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718925.1"
                     /translation="SPGGKKDSMTKNRITPDLRSFVAYASEKFCCPVVCRLRSFYLPY
                     SCLFPTGGNGQPEKSDRQGGK"
     gene            178..2094
                     /locus_tag="VFU15_13875"
     CDS             178..2094
                     /locus_tag="VFU15_13875"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU4718926.1"
                     /translation="MIGREENDTSKVNALNRLAFLLRSSDQDTALNYATQAKDISDAL
                     RYEKGQADACNVIGIIDYRRGDYIGAIRMHMRALDIRERTGDQKGAALSYINLGNVYS
                     DQNQNAMAQQQYLAAVDILKKEKDHGRLAIVYLDIGTIYLSQNNDSAGLEYNRMAMDE
                     AQLAGKTDVEGQALNNMGVAYEAEGKFGPASVMYRKAYGIACADSDKVAMTDAAINLG
                     NISRKTKNIRGAFDWHFKAEQYARSNGYLEGIRVLYEEISNDYRDTGDFEKALYYHTR
                     FKEVSDSLFNDENTTKIIELTNRWQGAEREKELMESRNEIAQRTEAERKANQRLWIAG
                     TGAVFLLFFAGYVFYAYSRNRRAGLLLAAQKDEIERKSEELEMKNRDITDSIVYSRRI
                     QRAMLPSEQKFRELLPDSFVFYKPRDIVSGDFYWVESWGDEILVAAGDCTGHGVPGAL
                     LSVVGLNLLNQTLQVYGITKPAAVLNALNRGMAQALGQKYSSVPSRDDPFEVSDGMDI
                     ALVTINRKTAKLEFAGANNPVWLCRGGVITEYRGDRQPVGQYIPEMQKPFTSHEAELR
                     KGDVIYLFSDGFADQFGGDKGKKYRLSKMKALFEKHSHLPMQQQLEMLEKEFSLWKGD
                     LGQVDDVLVIGVKV"
BASE COUNT          559 a          610 c          668 g          458 t
ORIGIN      
        1 gatctccagg cggtaagaag gacagcatga ccaaaaacag gattacgcct gacttacgta
       61 gctttgtcgc ctatgcctcg gaaaagttct gttgtcctgt tgtttgccgc ctgcgcagtt
      121 tttatctgcc gtattcctgc cttttcccaa caggcggaaa cggacagcct gaaaaaagtg
      181 atcggcaggg aggaaaatga tacatcgaag gtcaatgcgc tcaaccgtct tgcgttcctg
      241 ctgcgttctt ccgaccagga tacggcgctg aactacgcca cgcaggcgaa ggatatttcc
      301 gacgcgctcc ggtacgaaaa aggccaggcg gatgcctgca acgtgatcgg cattatcgat
      361 taccgccgcg gtgattacat cggggcgata agaatgcaca tgcgcgcgct cgatatacgc
      421 gagcgtaccg gcgatcagaa aggcgctgcg ctcagctaca tcaacctcgg gaatgtttac
      481 agcgaccaga accagaacgc tatggcgcag cagcagtacc tcgctgccgt ggacatcctg
      541 aaaaaagaga aagaccacgg ccggctggcg atcgtttacc tcgatatcgg caccatttac
      601 ctttcgcaga acaacgacag cgccggcctc gaatacaacc gcatggcgat ggacgaagcg
      661 cagctggcgg gaaaaacaga tgtcgaggga caggcgctga acaatatggg tgtggcgtat
      721 gaagcggaag ggaaattcgg tccggcctcc gtcatgtaca ggaaagcgta cgggatcgcc
      781 tgcgccgaca gcgacaaagt ggcgatgacc gatgcggcga tcaacctcgg gaatatttca
      841 cgtaaaacga aaaatatccg gggcgcattc gactggcatt tcaaagcaga gcaatatgcg
      901 cgcagcaacg ggtacctcga agggatacgc gtgctgtacg aggagatctc gaatgattat
      961 cgtgatacgg gcgatttcga aaaagcgctg tattaccaca cacgtttcaa ggaagtgagc
     1021 gattcgcttt tcaacgacga gaataccacg aagatcatcg agctgacgaa ccgctggcag
     1081 ggtgcggagc gggaaaaaga gctgatggaa agcaggaacg agatcgcgca gcggacggag
     1141 gcagaacgga aggcgaacca gcgtctctgg atcgcgggaa cgggtgctgt ctttcttttg
     1201 ttcttcgcgg gatatgtttt ttatgcctac agcaggaacc ggcgggccgg cctgctgctg
     1261 gccgcgcaga aagacgagat cgaacggaaa agcgaagagc tcgagatgaa gaaccgcgac
     1321 atcaccgaca gcatcgtcta ctcgcggagg atacagcgcg cgatgctgcc atcggaacag
     1381 aagttccgcg agctgcttcc cgattcattc gtgttctata aaccgcgtga tatcgtcagt
     1441 ggtgattttt actgggttga atcgtggggc gatgaaattc tcgtcgcagc cggtgattgc
     1501 acgggccatg gcgtgccggg cgcgctgctg agcgtcgtcg ggctcaacct gctcaaccag
     1561 acgctgcagg tgtacggcat cacgaaaccc gcagcggtgc tcaacgcgct caaccgcggg
     1621 atggcgcagg cgctcggaca aaaatattcg tccgttccct cgcgcgatga tccgttcgag
     1681 gtgagcgacg gcatggacat tgcgctggtc acgatcaacc ggaaaacagc aaagctcgaa
     1741 ttcgcgggcg caaacaaccc ggtatggctc tgccgcggag gagtcatcac cgaataccgg
     1801 ggcgaccggc agccggtggg acaatacatt cccgaaatgc agaagccgtt caccagtcat
     1861 gaagcggagc tccgcaaagg cgatgtcatc tatctttttt cggacggatt cgccgatcag
     1921 ttcggcgggg acaaagggaa aaaataccgc ctgtcgaaaa tgaaagcgct gttcgaaaaa
     1981 cattcgcacc tcccgatgca gcagcagctg gaaatgctgg aaaaagaatt ctcattatgg
     2041 aaaggcgacc tcggccaggt ggacgacgtg ctggtgatcg gcgtgaaggt ctgacgcgga
     2101 tttcactacc ttttcgccga tgaaaaaact tccgctgctt atcctgcttt cgctccccgt
     2161 ttcccttctt ttttcgcaga ccggcatcgg catttacccg ctgtccggga tggtttccgt
     2221 ccgctggaac ccgcatcacg tggtgcgcga ttcatccggc gtttctttcg ttcccgatta
     2281 tcattttcgt ctcga
//
LOCUS       DASTSF010000481         8240 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_82075,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000481 DASTSF010000000
VERSION     DASTSF010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 8240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8240
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82075"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            265..747
                     /locus_tag="VFU15_13880"
     CDS             265..747
                     /locus_tag="VFU15_13880"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU4718927.1"
                     /translation="MFTSFSLFTPFSAKHVLCGSKNTVKTGTDTYWLSVESSPTGLTR
                     FQHFLLFLEERWQISACNFLDIHLSVTEAIMNAIDHGNKGDANKSVYVCASQDENYFT
                     FTIEDEGDGFNYRRIKNPTDPENRLLPGGRGIFIMNYFSDDLRFADEGRSVKLLFAKK
                     "
     gene            complement(806..1969)
                     /locus_tag="VFU15_13885"
     CDS             complement(806..1969)
                     /locus_tag="VFU15_13885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEU4718928.1"
                     /translation="MARILIIDDEKSIRRTLREILEYENFKVEEAADGVEGLAFAQKE
                     KFDIILCDIKMPKMDGMEALDKLMEAAVDAPVIMISGHGNIETAVEAVKKGAYDFIQK
                     PLDLNRLLVTVRNALDKSKLVTETKTLKKKISKTFDMIGESEAITKIKDMIDKVAPTD
                     ARVLITGENGTGKELVARWLHEKSQRAGGPMIEVNCAAIPSELIESELFGHEKGAFTS
                     AVAQRKGKFELAEGGTIFLDEIGDMSLSAQAKVLRALQENKITRVGGDKEIKVNVRVL
                     AATNKDLKKEILKGNFREDLYHRLSVILIHVPSLNERKDDIPLLADHFLKQLNEENGM
                     PLKTVTKEAIRELQKINWTGNIREFRNVIERLVILADKTITDKDVVAYAKPKE"
     gene            2242..4650
                     /locus_tag="VFU15_13890"
     CDS             2242..4650
                     /locus_tag="VFU15_13890"
                     /inference="COORDINATES: protein motif:HMM:NF026255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU4718929.1"
                     /translation="MRFLPLFLLFLFPQFLSASSVKGKVTDAENKPMEFVVVTLLRAQ
                     DSSLVKGAVTDQDGAFSFDAAAGKYLVCASFTGYTKTWNGPFDVTDGQDLTVNDIVLQ
                     ASKELDAVTISDIQPLYIQKPDKLIMNVENSPVRITGTAWDLVTTAPGVSVDNNNNIS
                     LRGKSGVKVYIDGRNTYLSGDQLVSYLQNISAADVVQVEIISNPSAKYDAEGSGGIIN
                     VITRKGSQQGLNGTIRPGIGQGFYTRYEFGADFNYAKEKFNVYGKYFFANSNNANNFN
                     INRNVPYDGITTNFNQHSFSGNVPLGHRVSLGVDFYAKHNITWGGRIDAALAGEKDYT
                     QNSTVISVAGSDTSSLLYQVNHEKSFYNNGAANLYFSQKYDTLGKELSASADYLAYQI
                     GSHNDFDLNYYDNFGNHIAPEEFQRNISYSDIRIFVGQVDYSNPFGRKYKMETGLKSS
                     YVQTYNDLHFDLLDSSSSWFNDTTKSNRFIYREQINAAYVNGYADFGRWQFEAGLRAE
                     QTISDGNSPTTGEHLHRTYAQLFPSVFILEKLDDKNSLNYTYSHRINRPSYQDLNPFT
                     SYLDKYTYEKGNPFLQPEIADNAEFTYSYLDALFVTLGGSRTKFAMTDVTQQVDSTAI
                     GYKTTVNLDNVYSGYFGVSCPVPIGKWFMMENDLNLSYNQYKSDLFGTLVDNKSWMFN
                     GSSNLTFRLPQEWKLQLWGWYNSPGTYGIFRMKARGGVGGGISKTMLNRQLQFNLTVQ
                     DLFRTTGMHATINFQNQDAYVEFRPDSPRVYLRVRYSFGNSKATRKAQSKTGADDLKD
                     RTGK"
     gene            4854..5444
                     /locus_tag="VFU15_13895"
     CDS             4854..5444
                     /locus_tag="VFU15_13895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HEU4718930.1"
                     /translation="MENTYFAHPSAVIDEGCTIGQGTKIWHFSHIMPNCVIGEGCNIG
                     QNVVISPEVVLGKNVKVQNNVSLYTGVTCGDDVFLGPSCVFTNVTNPRSAVNRRSQYA
                     KTHVGKGATIGANATIVCGHDIGEYAFIGAGAVVTKNVPAYALVVGNPAKQTGWMSEY
                     GHKLNFSRDGIAVCPESKQEYELKNGAVHKTGKTES"
     gene            5520..6539
                     /locus_tag="VFU15_13900"
     CDS             5520..6539
                     /locus_tag="VFU15_13900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU4718931.1"
                     /translation="MIKFAIIGQGHIGKRHAEMVRRNPDAELVAVCDILPKEKTGNEK
                     IAEPYYASEVEMLKAHPEAEVVTICTPNGLHARQAMVALENGKHVVVEKPMALHRSDC
                     ELLLHKALSAGRQVFCVMQNRYSPPSVWLKEVVEKKIIGDIFLVQLNCYWNRDERYYK
                     KNTWKGTQELDGGTLFTQFSHWIDLLYWLFGDITEIQAKFADFTHRDLTDFEDSGFVN
                     FRFLNGGIGCINYSTAVWDKNLESSITVVGSRGSLKVGGQYMDKVEYCHIRDYVMPSL
                     EETAPANDYGAYKGSANNHHYVISNVIDTLSGKTSISTNALEGMKVVDIIERIYSLRK
                     PDRKS"
     gene            6619..7905
                     /locus_tag="VFU15_13905"
     CDS             6619..7905
                     /locus_tag="VFU15_13905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008771971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HEU4718932.1"
                     /translation="MYKNIIDRKEKIAVIGLGYVGLPIALAFAKKVKVIGFDINEKRV
                     AMMKKGIDPSNELSADAFKGCDIEFTASLDKLREAKFFVVAVPTPIDERNLPDLKPLL
                     GASASVGKVLKKGDYVVFESTVYPGCTEEDCIPVLEKESGLKFRKDFKVGYSPERINP
                     GDKEHTITKILKVVSGCDDESLDEIAKVYEIIVEPGTHRAPSIKVAEAAKIIENTQRD
                     VNIALMNELSLIFSKMGINTYDVLAAAGTKWNFLKFSPGLVGGHCIGVDPYYLTHKAE
                     SLGYHSRVINSGRYVNDSMGFYVAKTTVKKIIAEGKNIAQSKVLVMGATFKEDVSDIR
                     NSKVADIVKELKSFGVEVEVADPHADSDELKHEYGFGLNRIGKGYDGVIVAVNHKEYK
                     NLDENFFAGIMTKHGVLADVKGLYRGKIKKLSYWSL"
     gene            complement(7885..>8240)
                     /locus_tag="VFU15_13910"
     CDS             complement(7885..>8240)
                     /locus_tag="VFU15_13910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU4718933.1"
                     /translation="IFAQIWEQPQNSSRFLLLDEPFTYLDLGQQYLFAEQLRLFTGEK
                     TVTVLVMHDLQLAMQVADHIYVIHEGELIADGNPHHIITQKLLEDVYGIKAEIAEVDG
                     RKIILVQKSSEAPVG"
BASE COUNT         2062 a         2280 c         2140 g         1758 t
ORIGIN      
        1 tctttgatcc cgcccgcctg ctttccgcag gaaaaatttt tgcaggacgg ggtctgtgcg
       61 cctcgcgcca cggcctggca ccgggcacaa agcgaagaag gtgaaattct gcctattcac
      121 cgggccgcgc attccgcgca aaattttcca aaatcttaca tgtgtatgct cccgcatttt
      181 cgtgcagaaa cttcactgtt agcagggcgt aaaattgaag tgaaaaacaa ggaccctttt
      241 actcaccaaa aacctcttca ctttatgttc acctcctttt ccctcttcac gccgttttca
      301 gccaaacacg tcctctgcgg cagcaaaaac accgtaaaaa ccggcaccga cacctactgg
      361 ctgtccgtcg aatcttcgcc tacagggctg acaaggttcc agcatttcct ccttttcctc
      421 gaagagcgct ggcagatctc cgcgtgtaat tttctcgaca tccatctttc cgtcaccgag
      481 gcgataatga acgccatcga tcacgggaac aaaggtgatg cgaacaagag tgtttacgtc
      541 tgcgcgtcac aggatgagaa ttatttcacg ttcaccatcg aggacgaagg cgacgggttc
      601 aactaccgcc gcatcaaaaa cccgacggac cccgagaacc ggctgctgcc gggcgggcgc
      661 ggcattttta tcatgaacta tttttcagat gatctccgct ttgccgacga aggccggagc
      721 gtgaaattgc tgtttgcgaa gaaataacac ggggtaacgc gcagaacatt cgcgcacatc
      781 ccgggaaagc cgtaacgcgt atccgctatt ctttcggttt cgcgtacgcc accacgtctt
      841 tgtcggtgat ggttttgtcg gcgagaatga cgagacgttc gatgacgttg cggaactcgc
      901 ggatattgcc cgtccagttg atcttttgca gttcccggat cgcttccttc gtaacggtct
      961 tcagcggcat gccgttctct tcattcagct gcttcaggaa atggtccgcc agcaggggaa
     1021 tgtcatcttt ccgctcgttc agcgaaggaa cgtggatgag aataacgctg agacggtgat
     1081 acaaatcctc gcggaaattt cccttgagga tttctttttt gaggtctttg ttcgtcgcgg
     1141 cgagcacacg cacgttgact tttatttcct tgtcgccgcc cacgcgcgtg atcttgtttt
     1201 cctggagcgc acgcagcact ttcgcctggg cgctcaggct catatcgccg atctcgtcga
     1261 ggaaaatggt gccgccttcg gcgagctcga atttcccctt ccgttgcgca actgccgagg
     1321 tgaacgcacc tttttcatga ccgaacaatt ccgattcaat gagctccgac ggaatggccg
     1381 cgcagttcac ttcgatcatc ggtccgccgg cgcgctggct cttttcgtgc agccatcgcg
     1441 cgaccagctc ttttcccgtt ccgttctcgc ccgtgatgag cacgcgtgcg tccgtgggcg
     1501 cgaccttatc gatcatgtcc ttgatcttcg tgatcgcttc cgattcgccg atcatgtcga
     1561 acgttttgct gatcttcttc ttcagcgttt tcgtttcggt gacgagcttc gatttgtcca
     1621 gcgcattgcg cacggtgacg agcaggcggt tcaggtcgag cggcttctgg ataaaatcgt
     1681 acgcgccctt tttcactgct tccacggccg tttcaatgtt cccgtgaccg gagatcatga
     1741 tgaccggtgc atcgacggca gcttccatca gcttgtcgag cgcttccatt ccgtccatct
     1801 tcggcatttt gatatcgcag agaatgatgt cgaatttttc cttctgcgcg aacgcaagtc
     1861 cttccacgcc gtccgccgct tcctccactt tgaaattttc atattcaagg atttcacgca
     1921 gggtacggcg aatgcttttt tcgtcgtcaa taatgagaat acgggccata atgatcaggt
     1981 gagaagggta aaaatacgaa ttcaggaatt agcgcgtgag gccgactgaa aatgcagccc
     2041 cgctgctcac cgctcccggc aacggagatg cagcgcgaat gcagggacag ccgcacccgc
     2101 gatgggggac ggcatcagcc ggggagcaaa aattatttcc ggcctgtctt ccataacgca
     2161 gattaacgca gcgcaggtaa atctttattg cccgtcaaaa taaactccga actttacgcg
     2221 tctttaatgc aaacgccgca catgcgcttt ctcccgctct tcctgctttt tctttttccg
     2281 caatttttgt ccgcctcttc cgtgaaagga aaggtgaccg atgcagaaaa caagccgatg
     2341 gaattcgttg tcgtgacgct cctgcgcgcg caggattcct cgctcgtaaa aggagccgta
     2401 accgaccagg acggcgcgtt ctcattcgac gctgccgccg gaaaatacct cgtctgcgct
     2461 tccttcaccg gttacaccaa aacgtggaac ggaccgttcg acgttaccga cgggcaggac
     2521 ctcaccgtga acgacattgt cctgcaggct tcgaaagagc tcgacgccgt gacgatctcc
     2581 gacatacagc cgctttatat ccagaagccc gacaagctga tcatgaacgt cgagaacagt
     2641 cccgtgcgca ttacgggaac agcgtgggac cttgtcacca ccgcgcccgg cgtaagcgtg
     2701 gacaacaaca acaatatttc cctgcgcgga aaatcaggcg tgaaagttta catcgacggg
     2761 cgcaatacct acctgtccgg cgaccagctc gtgagctacc tgcagaatat ttccgcggcg
     2821 gacgtcgtgc aggtggagat catcagcaac ccttcggcga aatacgatgc ggaaggcagc
     2881 ggcggcatca tcaacgtcat cacgcgaaaa ggatcgcagc agggactcaa cggaacaata
     2941 cgtcccggca tcgggcaggg attttacaca cgttatgaat tcggcgccga cttcaattac
     3001 gcgaaagaaa aattcaacgt gtacggaaaa tattttttcg cgaacagcaa caacgccaat
     3061 aatttcaaca tcaaccggaa cgtaccgtac gacggcatca ccacgaattt caaccagcat
     3121 tcgttttcgg gcaacgttcc cctgggccac cgcgtttcgc tcggcgtcga tttttatgcg
     3181 aagcacaaca tcacctgggg cgggcgcatc gatgcggcgc tggccgggga aaaagattac
     3241 acgcagaaca gcaccgtgat ctcggtcgcc ggaagcgaca cctcttccct gctctaccag
     3301 gtgaaccacg aaaaaagctt ttacaacaac ggggcggcga acctttattt ttcgcagaaa
     3361 tacgacacgc tgggcaaaga gctttccgca agcgccgatt atctcgccta ccagatcggg
     3421 tcgcacaatg attttgacct gaattactac gacaatttcg ggaaccacat cgccccggaa
     3481 gaattccagc gcaacatttc ctacagcgac atccgcattt tcgtgggaca ggtggattac
     3541 tcgaacccgt tcggcaggaa atacaagatg gaaaccgggc tgaagagtag ctacgtgcag
     3601 acgtacaatg accttcattt cgacctgctc gacagcagtt ccagctggtt caacgacacc
     3661 acgaaatcga accgcttcat ttacagggaa cagatcaatg ccgcttacgt caacggctac
     3721 gccgatttcg gaaggtggca gttcgaagcc ggactgcgcg cggaacagac gatctccgac
     3781 ggcaattcac cgacaacggg cgagcacctg caccgcacgt atgcacagct tttccccagc
     3841 gttttcattc tcgaaaagct ggacgataaa aattcgctca actacacgta ttcgcaccgc
     3901 atcaaccggc cttcgtacca ggacctgaac ccgttcactt cctatctcga caaatacacg
     3961 tacgaaaaag gaaacccgtt cctgcagccg gagatcgcgg acaatgcgga gttcacctat
     4021 tcgtacctcg atgcgctgtt tgtcaccctt ggcggaagcc gcacgaaatt cgccatgacg
     4081 gacgttacgc agcaggtgga ttccaccgcg atcggctaca aaacaacggt gaacctcgat
     4141 aacgtgtaca gcggttattt cggcgtttca tgtcctgtac cgatcgggaa atggttcatg
     4201 atggagaacg atcttaatct ttcgtacaac cagtacaaat cggatctctt cggcacgctg
     4261 gtcgacaaca aaagctggat gttcaacggc tcctcgaacc tcacgttccg cctgccgcag
     4321 gaatggaagc tgcagctctg gggctggtat aattcccccg gcacgtacgg aattttccgt
     4381 atgaaagcga gaggcggcgt gggcggagga atttccaaaa caatgctcaa ccggcagctg
     4441 cagttcaacc tcacggtaca ggatcttttc cgcacgacgg gaatgcacgc gacgatcaat
     4501 ttccagaacc aggacgcata cgtcgagttc cgccccgatt ccccgcgtgt gtacctgcgc
     4561 gtgcgttact cgttcggaaa ttccaaagcg acgcgcaaag cgcaatccaa aacaggcgcg
     4621 gacgacctga aagaccgcac gggaaaataa gtccggtcag cggtcccggg tttgggtgcc
     4681 tcgtaccggg gatccgcgca gcttgttatt ttccctgatc cgctgcgtaa aaaccaacgc
     4741 acatactgaa aaaacggccg ggaaccattg cctgcctttc gtccccgggt attcactgaa
     4801 aaacacggca caggtgtttt ccgattggtt atcttagtgc ctcttttctc cttatggaaa
     4861 acacctattt cgcccacccg agcgccgtca tcgacgaagg ttgtacgatc ggacaaggga
     4921 cgaagatctg gcacttctcg cacatcatgc cgaactgcgt gatcggcgaa ggctgcaaca
     4981 tagggcagaa cgtggtgatc tcgccggaag tcgtgctcgg aaaaaacgta aaagtgcaga
     5041 acaacgtttc gctgtatacc ggcgtcacat gcggcgacga tgtttttctc ggcccgtcct
     5101 gcgtgttcac gaacgtcacc aacccgcgca gcgcggtgaa ccggcgctcg cagtacgcaa
     5161 agacccacgt gggaaaaggc gcgaccatcg gcgcgaacgc gaccatcgtg tgcgggcatg
     5221 atatcggcga atatgcgttc atcggtgccg gcgccgtggt cacgaaaaac gttcccgcct
     5281 acgcgctcgt cgtgggaaat cccgcgaagc aaacagggtg gatgagcgaa tatggccaca
     5341 agctgaattt cagcagggac ggcatcgcgg tttgcccgga gtcgaaacag gaatacgaac
     5401 taaaaaacgg ggcggtgcat aaaaccggga aaacagaaag ctgatccgga agcagcattc
     5461 atcgctgcac tttccaactt tccaactgaa tgatttcaac tttccaactt ctgaattgaa
     5521 tgataaaatt cgccatcatc ggccagggac atatcggcaa gcggcacgcg gaaatggtac
     5581 gcaggaaccc ggacgcagag ctggtggccg tgtgtgatat tctcccgaaa gaaaaaacag
     5641 gcaacgaaaa gatcgcggaa ccgtattacg catcggaagt cgaaatgctg aaagcgcacc
     5701 cggaagccga ggtggtgacc atctgcacgc cgaacgggct gcacgcacgg caggccatgg
     5761 tggcgctcga aaacgggaag cacgtcgtgg tggaaaaacc gatggcgctc caccgcagcg
     5821 actgcgagct gctgctgcac aaagcacttt ccgccggacg ccaggttttc tgcgtgatgc
     5881 agaaccggta ttcaccccct tccgtgtggc tgaaggaagt ggtggagaaa aaaataatcg
     5941 gcgacatctt tctcgtgcag cttaactgct actggaaccg cgacgaacgt tattacaaaa
     6001 aaaacacgtg gaaaggaacg caggagctcg acggcggaac gctgttcacg cagttctcgc
     6061 actggatcga cctgctgtac tggctcttcg gcgacatcac tgaaatacag gcgaagttcg
     6121 ccgacttcac gcaccgcgat ctcaccgact ttgaagattc cggctttgtc aatttccgtt
     6181 tcctcaacgg cggaatcggg tgcatcaact attccaccgc ggtgtgggac aaaaacctcg
     6241 aaagcagcat cacggtggtc ggcagcaggg gaagcctgaa agtgggcgga caatacatgg
     6301 acaaagtgga atactgccac atcagggatt acgtcatgcc ttcgctggaa gaaacggcgc
     6361 ccgcgaacga ctacggcgca tacaaaggct cggcgaacaa ccaccattac gtgatcagca
     6421 acgtcatcga cacgctctcg ggaaaaacca gcatctccac caatgcgctg gaaggaatga
     6481 aagtcgtcga tatcatcgag cggatctatt cgctgagaaa accggacagg aaatcctgaa
     6541 cgtttcccgg cagcaaaaca acaaacaacc accggcaaaa agcatccagt tataaatttg
     6601 agcacaaacg agatgagcat ctacaaaaac ataatcgaca ggaaagagaa aatcgccgtc
     6661 atcggtctcg gttacgtcgg gctgccgata gcgcttgcat tcgcaaaaaa agtgaaggtg
     6721 atcggtttcg acatcaacga aaaacgcgtc gcgatgatga agaaaggcat cgatccgagc
     6781 aacgagcttt cggcggacgc gttcaaaggc tgcgacattg aattcacggc ttcgctcgac
     6841 aagctgcgcg aagcgaaatt cttcgtcgtc gctgtcccca cgccgatcga cgagcggaac
     6901 ctgcccgacc tgaaaccgct gctcggcgcg tcggcgtcgg tgggaaaagt gctgaaaaaa
     6961 ggtgattacg tcgtgttcga atcgaccgta tatcccggct gcacggagga agactgcatt
     7021 cccgttctcg aaaaagaatc aggactgaaa ttcaggaaag atttcaaggt cggctactcg
     7081 cccgagcgta tcaatcccgg cgacaaggaa catacgatca cgaagatcct gaaggtcgtt
     7141 tccggctgcg acgacgaaag cctggacgag atcgcgaaag tgtacgagat catcgtggag
     7201 cccggcacgc accgcgcacc ttcgatcaag gtggcggaag cggcgaagat catcgagaac
     7261 acgcagcgtg atgtgaacat cgcgctcatg aacgagcttt cgctcatctt cagcaagatg
     7321 ggcatcaaca cgtacgacgt gctcgcggct gcgggaacga aatggaactt cctgaaattc
     7381 tcaccgggac tcgtgggcgg ccactgcatc ggcgtcgatc cgtattacct cacgcacaaa
     7441 gcggaatcgc tcggctatca ttcccgcgtc atcaattcgg gacgttacgt gaacgattcg
     7501 atgggatttt acgtagcgaa aacgaccgtg aagaagatca tcgccgaagg aaagaacatc
     7561 gcgcagtcga aagtgctcgt gatgggcgcg acattcaagg aagacgtgag cgacatccgc
     7621 aactcgaaag tcgccgacat cgtgaaggaa ttgaaatctt tcggcgtgga agtggaagtg
     7681 gccgatccgc atgccgacag cgatgagctg aaacatgaat acggcttcgg gctgaacagg
     7741 atcggcaaag gttacgacgg cgtgatcgtc gcagtgaacc acaaggaata caaaaacctc
     7801 gatgaaaatt tcttcgccgg catcatgaca aaacacggcg tgctcgccga tgtaaaaggg
     7861 ctgtaccggg ggaagataaa gaagctatcc tactggagcc tctgatgact tttgtacaag
     7921 aattattttt cttccgtcaa cttctgcaat ttctgctttt atcccgtaaa catcctccag
     7981 gagcttttga gtgataatgt ggtgcggatt tccatcagcg atcaattccc cttcgtgaat
     8041 gacataaata tgatctgcaa cctgcatagc cagctggaga tcgtgcatga cgagcaccgt
     8101 tacggttttc tcacctgtaa acaggcgcag ttgttcggca aaaagatatt gttgcccgag
     8161 atcgagataa gtgaatggct cgtcgagtaa taagaatcgt gagctgtttt gtggctgttc
     8221 ccatatctgt gcaaagatgc
//
LOCUS       DASTSF010000482         7760 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1773838,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000482 DASTSF010000000
VERSION     DASTSF010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 7760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7760
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1773838"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            268..1173
                     /gene="gldN"
                     /locus_tag="VFU15_13915"
     CDS             268..1173
                     /gene="gldN"
                     /locus_tag="VFU15_13915"
                     /inference="COORDINATES: protein motif:HMM:TIGR03523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldN"
                     /protein_id="HEU4718934.1"
                     /translation="MAIVRTAGNELSVALKVLFLFIALFLFARVHAQSFNPCAPQEAS
                     MIIGPGAHGASLTRQPVPYPYVREADVMWSKRIWRTIDLREKMNFPYYFPEKPHNGLA
                     SLFDIIKGAVMHGCLTAYDNPAMDDEFKVKMSMQQVKDLLVSVSIIDVEDPLNPGTYK
                     KDTIPEEINSTDVMAYWLKEDWFFDKERSVMDVRILGICPLATKKDPTTGEILGYKPL
                     FWVYFPELRPIIAKQEVFLGQNFSQRLTYDDMFQKRFFSSYIRKESNVYDRSIPEYAT
                     GLDALLESEHIKEGIMDFESDLWHY"
     gene            complement(1264..1926)
                     /locus_tag="VFU15_13920"
     CDS             complement(1264..1926)
                     /locus_tag="VFU15_13920"
                     /inference="COORDINATES: protein motif:HMM:TIGR02453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 domain-containing protein"
                     /protein_id="HEU4718935.1"
                     /translation="MIKKETLGFLSRLKKNNNRDWFEKNKQLYLDAKENVENTVEEII
                     AEVRKIDRRIPAELSARKCMFRIYRDIRFSKDKRPYKTNMGAMINPGGKMASVPGYYI
                     HFEPGRTFLAGGIYMPGPAELAKIRQEIDYNFRDFKKIVSDKSFKKYFGELDQDDKLV
                     TTPKGYAKDHPAIEFLKLKSFIVVAEISEKDTLSKNFAKTVAPVFKAMQPLHKFLQQA
                     VD"
     gene            complement(1923..3062)
                     /locus_tag="VFU15_13925"
     CDS             complement(1923..3062)
                     /locus_tag="VFU15_13925"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEU4718936.1"
                     /translation="MRNNVLTGIFIFFSLVALVALGWMFWFLRTVFLYMIIAAVLTVM
                     TRPLGNLIDKIRFRKKKVPRGIRSFLILISIYLVLFGFVSIFIPLITNEANILSNVSS
                     KQLSQALHEPIRQVQDVFASYQQSSGDTQSLDQSIQKVTGEMLNAAQVSSIANTVASI
                     LGNAIVALLAISFFTFFFVKDGPVIFETLLLLVPPKHIQAIRNISDNSQVMLSKYFTG
                     VLLDIVFVTTFVSSGLAILGVPNPLIIGFFAGIMNIIPYVGPLIGGAFAVVIGISANL
                     DLDFYNGLLPLAEKIILVFVLMNVTDAFLVQPFIFSKRVKAHPLEIFTVVLMAGKIAG
                     IGGMVVAVPTYTILRIVAREFFSKYRFVQRITNDLEKSTAKKTGI"
     gene            3213..5141
                     /locus_tag="VFU15_13930"
     CDS             3213..5141
                     /locus_tag="VFU15_13930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010662965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEU4718937.1"
                     /translation="MISVNNVTVSFGGFTLYDGISFLINPRDRIGLAGKNGAGKSTLM
                     KIIAGEQRPTSGDIAIPRECTVGYLKQDMAASLGKTVFDETATAFSEIRALEEKIAHI
                     GEQLTIRTDYESDEYMDLIQQLNDSNERLHVLDGGEINVQIERTLMGLGFERKDFTRL
                     TDEFSGGWRMRIELAKILLRKPNVLLLDEPTNHLDIESIQWLENFLVDYYGAVVIVSH
                     DKAFLDNLTTRTIEISLGKIHDYRANYSRYLELRKERRDTQMAAAKNQQKFIDKTEQL
                     IDKYRAKANKASFAQSLIKKLDKIEVIEVDDEDNTAIRFRFPPAPRSGKVVVEAKAAT
                     KAYGDKTIFSNVDFEIEREDRVAFVGRNGEGKSTMIRMINGEKATGGEVKTGFSVSMG
                     YFAQDDADMLDPDKTVFDTIDELAVGDVRKQVRGLLGSFLFSGDDIDKKVKVLSGGER
                     TRLALCRLLLKPHNFLVLDEPTNHLDMRSKDVLKQALLKFDGTLVVVSHDRDFLQGLT
                     SKVFEFRGGNVKQHIGDVYEFLRSRKIENFAELELKEKKQETKSPAEENPQADNEEYR
                     KKQKAIGQLKNRISKTEAEIGELEKQIAEVDHQLMDPEKYQSVVSDKEIFPRYEAMKK
                     KLEEKMAEWEKLNGELGE"
     gene            complement(5241..5903)
                     /locus_tag="VFU15_13935"
     CDS             complement(5241..5903)
                     /locus_tag="VFU15_13935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718938.1"
                     /translation="MKNMLFISALFVSAFSFAQTADSTYGMPPKFFEAMKKNVAVLDT
                     ATVKSTFQQSYNSFDRLNNAEKSQWLPSYYMAYCLLVESYMDETSKTDDYCDQASHLL
                     DHADSISPNNSEILVLRSMCASARIRVNPMTRGGKYGKESGQWLDKAQQADSTNPRIA
                     YLRGTGLLYTPPAFGGGKDKAKPVLQDALKKFDAFRPASPVHPNWGRKQAEMMLAQCD
                     QK"
     gene            complement(5930..>7760)
                     /locus_tag="VFU15_13940"
     CDS             complement(5930..>7760)
                     /locus_tag="VFU15_13940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU4718939.1"
                     /translation="LNVVTISAGTIEASDEKRTTVLKPLDIVTTAGASGDIYGALQTL
                     PGTSRVGDQGGLFVRGGTGAETKTVIDGLNVNNPFFSAVPDVAARGRFSPFLFKGTVF
                     STGGYSAQYGQALSSVLVLETQDLPDQSSSTLGISSVGIGGGHNHLWEKKGLSAGFDL
                     DYTNLGPYFAMNPQVQKYTRIPQFWGGSANFRKKTGEKGMLKFYGYFNYSDLALTHND
                     IDSTNGYQSPFDLHNLDSYNNLSWRQYIGTKWRLDMAGSWSYNLDRIISDADTIRTQD
                     VISQGKVMLTRYFMERNQLRVGSEYQYITEDGQYDSYKNFFCDNFVSAFAETDIFLGT
                     RFVARIGGRVENSSLLAKTNVAPRVSLAMKTGEHSQASVAYGDFYQKPERDQLYVDHN
                     VGYQKATHYILSFQHVDDSVTLRIEAYYKTYEDLLKTVPSYSNTGDGYARGIDVFFRD
                     KKTFRNVDYWISYTYLDTKRNYLDYPYSVMPTFASPHNASLVVKKFFPKIMTSVGFSY
                     FYSAGRPYFNPNNPVFLGDRTYDYHDINLNASYLTHIGKAFTVLVISVTNVTGFNQVY
                     GYRYSYDGTRREAIAPPSKRSIFLGMFMSFGQDRSKDVINNNN"
BASE COUNT         1909 a         1870 c         2196 g         1785 t
ORIGIN      
        1 gctgctcgaa tcggagcgcg tgaaggacga tattttccgg atggagcacg atgtctggca
       61 tctgtaggtc gtgcttttgc ggacggtttc cggctgaacc ccggccggga ccttttttcc
      121 caatgaaatt cagaaaaata attgccccgg ttcttcgtta ttagtagaca gaaccaaccg
      181 ggatgaataa atctacctca ctacacctga ctaaaaaaag caaaggaggc tgtcatgttt
      241 taccccggaa aaaagaaaaa tactgttgtg gcaatcgtgc gtacggcagg aaatgaactg
      301 tctgttgcgc ttaaagttct ctttctgttc atagcgctct ttctttttgc aagagtgcac
      361 gcgcaatcgt tcaacccgtg cgctccgcag gaagcatcga tgatcatcgg gcccggtgcg
      421 catggcgcca gcctgacgcg ccagcctgtt ccgtacccgt atgtgcggga agccgacgtc
      481 atgtggtcga aacgcatctg gcgcacgatc gatcttcgcg agaagatgaa tttcccgtat
      541 tatttcccgg agaagccgca caacggtctc gccagtcttt tcgatatcat caagggcgca
      601 gtgatgcacg gctgcctgac cgcgtacgac aatcctgcaa tggacgatga gttcaaagtg
      661 aaaatgtcta tgcagcaggt gaaagatctt ctcgtcagcg tcagcatcat cgacgtggag
      721 gatccgctga acccgggcac gtacaaaaaa gacacgatcc cggaagagat caacagcacg
      781 gacgtaatgg cgtactggct gaaggaagac tggttcttcg acaaggagcg ttccgtgatg
      841 gacgtgcgga tactcggaat atgcccgctg gcgacgaaga aggatccgac cacgggcgaa
      901 atcctcggtt ataagccgtt gttctgggtg tatttccccg aactgcgtcc catcattgca
      961 aagcaggaag tctttctcgg tcagaatttc tcacagcgcc tgacatacga cgacatgttc
     1021 cagaaacgtt ttttcagcag ttacattcgt aaagaatcga acgtgtatga ccgctccatc
     1081 ccggagtacg caacgggtct cgacgcgttg ctggaatcag agcatatcaa ggaaggaata
     1141 atggatttcg aaagcgacct gtggcactat taaagtcgcg ccatgttaaa aaaagaaaat
     1201 ccgggagagc atcccggatt tttttttgag cgttgcgttc gaacgcggga acagaatcca
     1261 ttttcagtcg acggcctgct ggagaaattt atgcaggggc tgcatggctt tgaagacagg
     1321 cgccactgtt ttggcaaagt tctttgaaag cgtgtctttt tcactgatct ccgcgacgac
     1381 gatgaagctt ttcagcttca ggaattcgat cgccgggtga tcttttgcat aacctttggg
     1441 cgtggtgacg agcttgtcgt cctggtcgag ttccccaaaa tattttttga acgatttatc
     1501 cgacacgatc tttttgaaat ccctgaaatt gtaatcgatc tcctggcgga tctttgcgag
     1561 ctctgcaggt ccgggcatat atattccgcc ggcaaggaat gttcttcccg gttcgaagtg
     1621 aatgtagtag ccgggcaccg acgccatttt tccgccggga ttgatcatcg cgcccatatt
     1681 cgttttgtaa ggacgtttgt ctttcgagaa gcggatatcg cggtaaatgc ggaacatgca
     1741 tttgcgtgcc gacagttctg ccggaatgcg cctgtcgatc ttccgtactt cggcgataat
     1801 ttcttccacc gtattttcta cgttctcttt cgcgtcgaga taaagctgct tattcttttc
     1861 gaaccagtcg cggttgttgt ttttcttcaa ccgggaaagg aagccgagag tttctttctt
     1921 gatcatattc ctgttttttt tgcagtcgat ttttcgagat cattcgtaat gcgctgcacg
     1981 aacctgtact tgctgaagaa ttcgcgcgcc acgatccgca ggatcgtata cgtgggaacc
     2041 gccacgacca ttccgccgat gccggctatt ttcccggcca tcagcacgac ggtgaaaatt
     2101 tccagcgggt gcgctttcac acgcttgctg aaaatgaacg gctgtacgag gaaagcgtcc
     2161 gtcacattca tcaaaacaaa cacaagaatt attttttcgg cgagcggaag aagcccgttg
     2221 taaaaatcga gatcaaggtt ggcggaaata ccgatcacca cggcgaacgc cccgccgatg
     2281 agcggcccga cgtacggaat gatgttcatg atcccggcga aaaagccgat gatcagtggg
     2341 ttcgggacgc cgagaatggc aagaccggaa gaaacgaacg tggtgacgaa aacgatgtcg
     2401 aggagaacgc cggtgaaata tttcgaaagc atcacctgcg aattatccga aatgttgcgg
     2461 atcgcctgta tatgtttcgg cggaacgagc agcagcagcg tttcgaaaat gacgggcccg
     2521 tccttgacga aaaagaacgt gaagaaagag atggcgagta aggcgacaat ggcgttcccg
     2581 aggatcgatg cgaccgtatt ggcaatggaa gaaacctgcg cggcgttcag catttcaccc
     2641 gtcactttct gtatggactg gtcgagcgac tgcgtatcgc cgctggactg ctggtagctg
     2701 gcgaatacat cctgcacctg ccggatcggt tcgtgaaggg cctgcgacaa ctgtttgctg
     2761 ctgacgttcg acaggatatt cgcttcgttc gtgatcagcg gaatgaaaat ggagacgaac
     2821 ccgaacagga cgaggtaaat gctgatgagg ataaggaagg aacgtattcc ccgcgggacc
     2881 ttttttttac ggaagcgtat tttgtcaatg agatttccga gcggacgcgt cattaccgta
     2941 agcaccgcag caatgatcat atacaggaaa acggtccgga gaaaccagaa catccacccg
     3001 agcgccacca gcgccacgag gctgaagaag atgaaaattc cggtaaggac gttattgcgc
     3061 attggctaaa agaggaataa aagtagaaat aacagccgac taaaccggga agccggtgaa
     3121 tgaatttgaa cctgttaagg aacccttaat acagggaaaa atgagatagc ttcctccgga
     3181 aaaggctgtt attgccgtac ctttgcgccc gcatgatttc agtgaacaat gtaacggtga
     3241 gtttcggcgg ttttacgctg tacgacggga tctccttcct tatcaacccg cgcgaccgca
     3301 tagggctggc ggggaagaac ggggcgggaa aatccacgct gatgaagatc atcgccgggg
     3361 agcagcgccc gacgagcggg gatatcgcca ttccgcgtga atgcacagtg ggctacctga
     3421 agcaggacat ggccgcaagt cttggcaaaa cggttttcga tgaaaccgca accgcttttt
     3481 ccgagatacg cgcgctcgag gaaaagatcg cgcacatcgg tgagcagctg acgatacgca
     3541 ccgattacga atcggatgaa tacatggacc ttatccagca gctcaacgat tcgaatgagc
     3601 ggctgcatgt gctggacggt ggcgagatca atgtgcagat cgaacggacg ttgatggggc
     3661 tgggcttcga gcggaaagat ttcacgcggc tgacggatga attcagcggg ggatggagaa
     3721 tgcggatcga gctggcaaaa attctcctgc gcaaaccgaa cgtgcttctc cttgacgagc
     3781 cgacgaacca cctcgacatc gaatcgatcc agtggctcga aaattttctc gtggattatt
     3841 acggcgccgt cgttatcgtt tcccacgata aagcgttcct cgacaacctc accacgcgca
     3901 cgatcgaaat ctcgctggga aagatccacg attaccgcgc gaactattcg cggtacctgg
     3961 agctgcgcaa ggaacgccgg gatacgcaga tggccgcagc gaagaaccag cagaaattca
     4021 ttgacaaaac agagcagctg atcgacaaat accgggcgaa agcgaacaag gcttcctttg
     4081 cgcaatccct gatcaagaag ctggacaaga tcgaagtgat cgaagtggat gacgaggaca
     4141 atacagcgat ccgtttccgt tttccgcctg caccgcgttc gggaaaagtg gtggtggaag
     4201 cgaaggcagc gacaaaagca tacggcgata aaacgatctt ttccaatgtc gatttcgaga
     4261 tcgaacggga agaccgtgtt gctttcgtcg gaaggaacgg cgaaggaaaa tcaacgatga
     4321 tccgcatgat caacggggag aaagcgacag gcggcgaagt gaagaccggc ttcagcgtga
     4381 gcatgggtta tttcgcgcag gatgatgccg acatgctcga tccggacaaa accgttttcg
     4441 atacgatcga cgagctggca gtgggcgatg tgcggaaaca ggtgagaggc ctgctcggtt
     4501 cgttcctgtt cagcggggac gatattgata aaaaagtgaa agtgctttcc ggcggagaac
     4561 gtacgcgtct tgcgctttgc cggttgctgc tgaaaccgca taattttctc gtgctcgacg
     4621 aaccgacgaa ccacctcgat atgcgatcga aagatgtgct gaaacaggcg ctgctgaaat
     4681 tcgacgggac gctcgtggtg gtttcgcacg accgtgattt cctgcaggga ctgacgtcaa
     4741 aagtttttga attccgcggc ggcaatgtga agcagcatat cggggacgtt tatgaatttc
     4801 tgcggtcccg gaagattgaa aatttcgccg agctcgagct gaaagaaaaa aagcaggaga
     4861 caaaatcgcc tgcggaagaa aatccccagg ccgacaacga agaataccgg aaaaagcaga
     4921 aagccatcgg tcagctgaag aaccgcattt caaaaaccga agcagagatc ggggagctgg
     4981 aaaaacagat cgcggaagtc gatcaccagc tgatggaccc ggagaaatac caatctgttg
     5041 tcagtgacaa agaaattttt ccccgctacg aggcgatgaa gaaaaagctg gaggaaaaaa
     5101 tggcggaatg ggaaaagctg aacggggagc tcggggaata agtaacgccg gacgactttt
     5161 tttcaagagc gtccgctgtt tttcttcaaa acattccgaa caaaacaggt ttccttagac
     5221 tttatttttc gcgcagcggt ctatttctga tcgcactgcg cgagcatcat ttccgcctgc
     5281 ttccggcccc agttcggatg aacaggactt gcgggcctga acgcgtcgaa ttttttcagc
     5341 gcatcctgga gaaccggttt cgctttgtct tttccaccgc cgaaagccgg gggcgtatag
     5401 agcaatccgg ttccgcgcag gtaagcgatc cgcggattgg tggaatccgc ctgctgtgct
     5461 ttgtcgagcc attgtcccga ctctttcccg tatttcccgc cgcgcgtcat cgggttcaca
     5521 cggatacgcg cagaagcgca catggaacga agcacgagta tttcagaatt gttcgggctg
     5581 atggaatccg catgatcgag gagatgtgaa gcctggtcgc agtaatcatc cgttttcgat
     5641 gtttcgtcca tgtaggattc cacgaggagg caatacgcca tgtaatacga aggcagccac
     5701 tggctttttt cagcgttatt gaggcgatcg aaggaattgt acgattgctg gaaagtggac
     5761 ttcacggtag cggtatcgag cacagcgacg ttctttttca tcgcttcgaa aaatttcggc
     5821 ggcatgccgt atgtgctgtc ggcggtctgt gcaaatgaaa aggcagagac gaagagcgcg
     5881 gaaatgaaga gcatgttttt catggcgggt gaataaaatt ttagcgggat caattgttgt
     5941 tgttgatgac gtctttcgat ctgtcctgtc cgaagctcat gaacataccg aggaagatgg
     6001 aacgtttgga gggcggcgca atcgcttcgc ggcgcgtgcc gtcgtaggaa tagcggtaac
     6061 cgtacacctg gttgaagccg gttacgttcg tcaccgaaat gacgagcacg gtgaacgctt
     6121 tcccgatgtg cgtaaggtag cttgcgttga gattgatgtc gtggtaatcg tacgtgcggt
     6181 cgccgaggaa aacaggattg ttcgggttga aatacggccg tcccgccgaa tagaaatagc
     6241 tgaatcctac agaggtcatg atcttcggaa agaatttttt caccacgagc gatgcgttgt
     6301 gcggggaagc gaacgtgggc atgacggagt acggatagtc gaggtaattt cttttcgtgt
     6361 cgaggtacgt gtagctgatc cagtaatcga cgttgcggaa agtttttttg tcgcggaaaa
     6421 aaacatcgat gccgcgtgca taaccgtcgc cggtgttgga ataagacgga acggttttca
     6481 gcaggtcctc gtacgttttg taataggcct cgatgcggag cgtaacgctg tcgtcgacat
     6541 gctggaagct gaggatgtag tgcgtcgctt tctggtagcc gacgttgtga tcgacataga
     6601 gctggtcgcg ttcgggtttc tggtagaaat cgccgtaggc gacgctggcc tggctgtgtt
     6661 cgcctgtttt catggcaagg gaaacacggg gagcgacatt tgttttcgcc agcagcgagc
     6721 tgttctccac gcgtccgccg atgcgggcga caaagcgcgt gccgaggaaa atatccgtct
     6781 ccgcaaaagc cgaaacaaaa ttatcgcaga agaaattttt gtagctgtcg tactgcccgt
     6841 cttcggtgat gtactggtat tcgcttccca cgcgcagctg gttgcgttcc atgaaatagc
     6901 gtgtcagcat tacttttccc tggctgatca catcctgcgt gcggattgtg tcggcgtcgc
     6961 tgatgatcct gtcgaggttg tagctccagg aacccgccat gtcgagccgc catttcgtgc
     7021 cgatgtattg ccgccagctg aggttgttgt aggaatccag gttgtgcagg tcgaaaggcg
     7081 actggtatcc gttcgtgctg tcgatgtcgt tgtgtgtaag cgcgagatcg ctgtaattga
     7141 aatagccgta gaatttcagc attccctttt caccggtctt tttgcggaag ttggccgagc
     7201 ctccccagaa ctgcgggatg cgcgtgtatt tctgcacctg cgggttcatc gcgaaatacg
     7261 gaccgagatt cgtgtaatcg agatcgaagc cggcggaaag tccttttttc tcccagaggt
     7321 gattgtgccc gccgccgatg ccgacggaag aaattcccag cgtggaggaa gactggtcgg
     7381 ggaggtcctg cgtttcaagc accagcacgg aagaaagcgc ctggccgtat tgcgcagaat
     7441 agccgcccgt gctgaaaacc gtgcctttaa aaagaaacgg agaaaaccgt ccgcgggccg
     7501 cgacgtcggg aacggcagag aagaaaggat tgtttacgtt cagtccgtcg atgacggttt
     7561 ttgtttccgc gcctgttccg ccgcggacaa acaatccgcc ctggtcgccc acgcgtgaag
     7621 tcccgggcag cgtctgcagc gcgccgtaaa tatcgcccga agcgcctgcc gtggtaacaa
     7681 tgtcgagcgg cttgagcacc gttgttcttt tttcatcgga cgcttctatc gttcccgcgg
     7741 agatcgtcac gacattcagc
//
LOCUS       DASTSF010000483         2761 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_66512,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000483 DASTSF010000000
VERSION     DASTSF010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2761
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66512"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1917)
                     /locus_tag="VFU15_13945"
     CDS             complement(<1..1917)
                     /locus_tag="VFU15_13945"
                     /inference="COORDINATES: protein motif:HMM:NF024962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprB repeat-containing protein"
                     /protein_id="HEU4718940.1"
                     /translation="MKKTLLLSQVVLTLFVFSAKAFGQASACPAVTTGTVAPVCSGQC
                     VNLSATVQGTLQTNTYTVSSIPYSPYSYTTGTAVLVNIDDVWTPVINMPFCFEFFGNT
                     YNQLVIGSNGLISFNTAYANAFCQWSYTAPIPTNTEPMNTIMAPYHDIDPSIGSTSDT
                     RYQVYGTAPCREFVISWYHVPMFSAACNSMLATQQIVLHESTNIIDIYIENKPLCAGW
                     NGGRATEGIQNATGTVAFFVPGRNASQWSVTNDGYRFMPAGTPNYSLQWTGPSGVVGT
                     TATVNVCPTTTTTYTCTVTNTSCSGPIVVSATETVTVSSGITTSGTQNNVTCNGSCNG
                     SATTNIVSGTGPFTYVWSPAPGGGQGTGTATGLCAGTYSCTVSNPTGCSTTQVFTITQ
                     PPVITATQSQVNVTCNGSCNGSATVNAAGGNGTYSYNWSPAPGGGQGTPTATGLCAGT
                     YTCTISSPAGCSITKIFTITQPPAITATQSQVNVNCNGSCNGSATVNPSGGSGSFTYT
                     WSPAPGGGQGTGNATGLCAGTYTCTIADATAPGCSITKIFTITQPSAITATQSQVNVN
                     CNGSCNGSATVNPSGGSGSFTYTWSPAPGGGQGTGNATGLCAGTYTCTIADAAAPGCS
                     ITKIFTITQPPAITA"
     gene            complement(2014..>2761)
                     /locus_tag="VFU15_13950"
     CDS             complement(2014..>2761)
                     /locus_tag="VFU15_13950"
                     /inference="COORDINATES: protein motif:HMM:NF019205.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HEU4718941.1"
                     /translation="IEGKMAGVICFEDTERTRRWDISERRFALFIAQLIAQAIETDRK
                     RNLRKDLEQALGEKKVILAELNHRVKNNFSLMGEIVKAQEEHARDEYHRNLFIETRSR
                     LHSLSMIHQQLYRSESTGSVNFRDFLLDLAAQFRNTFSGNNLEISTLLQNVSLPVTKA
                     ALAGLLVDEMLSVICKKIIPGKERRMIIIQLNTIGNLVNIRVRSDQPGIRTDENEIMK
                     ALLLKMDAECTTGTDEGSYYAVSFPLNQIY"
BASE COUNT          602 a          730 c          752 g          677 t
ORIGIN      
        1 agcggtgatc gcaggcggct gcgtgatcgt aaatattttc gtgatggaac agcccggtgc
       61 agcagcgtca gcgattgtgc atgtgtacgt tcccgcacac aaacctgtcg cgttgcccgt
      121 tccctgtcca ccgcccggcg cgggcgacca tgtgtacgta aatgatccgg atccgccgga
      181 aggattcact gtcgcgctgc cattgcatga accattgcaa ttcacattca cctgcgattg
      241 cgtagcggtg atcgcagacg gctgcgtgat cgtaaatatt ttcgtgatgg aacagccggg
      301 tgcagtggcg tcagcgatcg tgcatgtgta cgttcccgca cacaaacccg tcgcgttgcc
      361 tgttccctgt ccgccgcccg gtgcaggcga ccatgtgtac gtaaacgatc ccgatccgcc
      421 ggaaggattc actgttgcgc tgccattgca ggagccgttg caattcacat tcacctgcga
      481 ttgcgtagcg gtgatcgcag gcggctgcgt gatcgtaaat attttcgtga tagaacagcc
      541 ggccggactg gatatcgtac aggtatacgt tccagcgcat aagcccgtcg cggtgggtgt
      601 tccctgtcca ccgccgggag cgggagacca gttgtacgag tatgttccgt ttccacctgc
      661 tgcatttacc gttgcacttc cattacagga accattacat gtaacattca cctgcgactg
      721 cgtggcagta attacaggcg gttgcgtgat cgtgaatacc tgcgtcgtcg agcagccggt
      781 cggatttgaa acagtgcacg agtatgtgcc agcacacaaa cccgtagccg tacctgttcc
      841 ctgccccccg cccggcgcgg gggaccacac atacgtaaat ggccctgtcc ccgaaacgat
      901 attggttgtg gcggaaccgt tgcagcttcc gttacatgtt acgttattct gcgttccgct
      961 tgtggtgatg ccgctgctga ccgtgaccgt ttccgttgca gaaacaacaa tgggcccgga
     1021 acaggaagtg ttcgtgaccg tgcaggtata agttgtggtg gtcgtggggc aaacgttaac
     1081 tgttgctgtt gttccgacaa cgccgctggg tcctgtccat tgcaacgaat agttgggagt
     1141 tcccgcaggc atgaaacgat acccgtcgtt cgtaacggac cattgcgacg cattacggcc
     1201 gggaacaaaa aaggcaacag ttcccgttgc attctgaatt ccttccgtgg cgcgccctcc
     1261 gttccatcca gcgcagagag gtttgttctc aatgtaaata tcaatgatgt tcgtgctttc
     1321 atgcagaacg atctgctgcg tggcaagcat cgagttgcag gcggcgctga acatgggaac
     1381 gtgataccag ctgatcacga actccctgca gggtgccgtg ccataaacct ggtaacgcgt
     1441 gtccgaagtt gaaccaatgg aaggatcgat gtcatgatac ggcgccatga tcgtattcat
     1501 cggttccgta ttggtaggaa tcggcgcggt atacgaccat tggcagaatg cgttggcgta
     1561 ggcagtgttg aacgaaatga gcccgttcga tccgatgacc agctggttgt aggtgttccc
     1621 gaaaaattca aagcagaacg gcatattgat caccggcgtc cagacgtcgt cgatgttgac
     1681 aagcaccgcg gttcccgtgg tataggaata gggactgtac ggaatgctgg aaaccgtgta
     1741 ggtgtttgtc tgcagtgttc cctgcaccgt agcgctaagg ttcacgcact gccctgaaca
     1801 aacgggagcg actgttccgg tcgttacagc aggacaagcc gaagcctgtc cgaaagcctt
     1861 cgctgaaaac acgaagaggg taagcacaac ctgggagagg agtagtgttt ttttcatgcc
     1921 ctgaattctg gttacatgca tttaatgcca taactaaaat aggggggaat accaaggcga
     1981 aaattagaag gatattaata agcttcgccg gttctaataa atctgattca gggggaagct
     2041 caccgcgtaa tagctgcctt catctgtccc ggtagtgcat tcggcatcca tcttcagcaa
     2101 taacgctttc atgatctcgt tctcatccgt ccgtatgccc ggctggtcgg atcttacccg
     2161 gatgttgacg aggttgccga tcgtgttcag ctgtatgatg atcattctcc gctcctttcc
     2221 cgggatgatc ttcttgcaaa tgaccgaaag catctcatcc acgaggagtc ctgccagggc
     2281 ggctttcgta acgggcagtg agacgttctg aagcagcgtt gaaatttcca ggttgttccc
     2341 cgaaaatgta ttcctgaatt gcgcagcaag atcgaggaga aaatcgcgaa aattgaccga
     2401 gcccgtcgat tccgacctgt acagctgctg gtggatcatg gaaagactgt gcagcctgga
     2461 acgggtctca atgaacaggt tgcggtgata ctcatcgcgt gcgtgttctt cctgcgcttt
     2521 cacgatctcg cccatcagcg aaaaattatt ttttacgcgg tgattcaatt cggcaaggat
     2581 cactttcttt tccccgagcg cctgctccag atctttccgc aggttccgct tgcggtccgt
     2641 ttctatcgcc tgcgcgatca gctgtgcgat gaacagcgcg aaacgtcttt ccgaaatatc
     2701 ccacctcctt gttctttccg tatcctcgaa gcaaatgacg cctgccattt tcccttcgat
     2761 g
//
LOCUS       DASTSF010000484         2624 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_85713,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000484 DASTSF010000000
VERSION     DASTSF010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2624
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85713"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            122..1162
                     /gene="recA"
                     /locus_tag="VFU15_13955"
     CDS             122..1162
                     /gene="recA"
                     /locus_tag="VFU15_13955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013663880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEU4718942.1"
                     /translation="MSKEAKETKEVNKEKLKALQLTIDKLEKTYGKGTIMKLGDDAVE
                     TMEVIPTGSLTLDIALGVGGFPKGRIIEIYGPESSGKTTLAIHAIAESQKQGGIAAII
                     DAEHAFDRFYAQRLGVDIDSLLISQPDNGEQALEIADNLIRSGAIDIIVIDSVAALTP
                     KAEIEGEMGESKMGLQARLMSQALRKLTATISKTGCCCIFINQLREKIGVMFGNPETT
                     TGGNALKFYASVRLDIRRAGQIKEGETATGNRARVKVVKNKVAPPFRTAEFDILFGEG
                     ISRVGEIIDLGVEHNIIKKSGSWFSYEDTKLGQGRDSVKQLFHDNPELMERIELQIRE
                     SVKNKEAEVVVS"
     gene            1296..1526
                     /locus_tag="VFU15_13960"
     CDS             1296..1526
                     /locus_tag="VFU15_13960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718943.1"
                     /translation="MKTSWFIKKGILFVPVSVAGAFLYLLTLAFCATVFLAVDRHSHS
                     VSDTLYGIFPFFVSAFTVLFWIASNTLKKSGA"
     gene            1526..2284
                     /locus_tag="VFU15_13965"
     CDS             1526..2284
                     /locus_tag="VFU15_13965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009579800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEU4718944.1"
                     /translation="MAFYDLSAEERKRHVEKISREMLADIEKGGTKKILRYFSDSDTY
                     IRKSAYIAAGRIYSAHPALRKKIIRLLGELLQSDDFYIRQTAVNAAGEIAMKDFGAVE
                     KFFDNSLFDPHHSVRNAVIGSIKKSGRKNPRPVLAWAKKFLHHPDKEVRREICHGIEL
                     RGRTHPQEILPLLAELQFDTTARVKNTLVHVIGQISYKKNCLPVVIPALSKWKNRELV
                     AKAIEEIIDVHDRYRNFSALTQKEAIDFIHKNLV"
     gene            2308..>2624
                     /locus_tag="VFU15_13970"
     CDS             2308..>2624
                     /locus_tag="VFU15_13970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718945.1"
                     /translation="MKKNLLPVIAAVPFLLFLCCSSPPDKSAALQEQIDSLQKKLDDT
                     YRPGFGEFMSGIQVHHAKLWFAGKEQNWKLADFELHEIMETVDDLKKYQSERRESDKL
                     IML"
BASE COUNT          688 a          687 c          684 g          565 t
ORIGIN      
        1 ggatattagg tattgttaat aaatattttc gcgtttttgt cgaacctatt gggtttggct
       61 atacttttgt ccgtgaatca atgccagtcc aattagtaag tacacgagaa acatcagacc
      121 tatgagcaaa gaagcaaaag aaaccaagga agtaaacaaa gagaagctga aagcattaca
      181 gctgacgata gacaagcttg agaaaacata cgggaaaggc accatcatga agctcggcga
      241 cgacgctgtg gaaaccatgg aagttattcc gacgggttcc ctgacgctcg acatcgcgct
      301 cggcgtgggc gggttcccga aaggacggat cattgagatt tacggaccgg aatcttccgg
      361 taaaaccacg cttgccattc atgcgatcgc agaatcgcag aagcagggcg gcattgcggc
      421 catcatcgac gcggagcacg cgttcgatcg cttttacgca cagcgtctcg gcgtggatat
      481 cgacagcctg cttatttccc agccggacaa cggggagcag gcgctcgaga tcgcggacaa
      541 cctgatccgt tccggcgcca tcgatattat cgtcatcgac tccgtggctg cacttacgcc
      601 gaaagcggag atcgaaggcg aaatgggcga aagcaaaatg ggattgcagg cgcggcttat
      661 gtcgcaggcg ctgcgcaagc tcacggccac gatcagcaag accggctgct gctgtatttt
      721 catcaaccag cttcgcgaga agatcggcgt gatgttcggc aacccggaaa cgacgaccgg
      781 cggaaacgcg ctgaagttct acgcttccgt gcgtctcgat atccgccgcg caggccagat
      841 caaggaaggc gaaaccgcca cgggcaaccg cgcacgcgtg aaagtggtga agaacaaagt
      901 cgctcctcct ttccgcaccg cggaattcga cattcttttc ggagaaggca tttcccgcgt
      961 gggcgagatc atcgacctcg gcgtggagca taacatcatc aagaagagcg gaagctggtt
     1021 cagctacgaa gacaccaaac ttggccaggg ccgcgattcg gtgaagcagc ttttccacga
     1081 caacccggaa ctgatggagc gcatcgaatt gcagatccgc gaatcggtaa agaacaagga
     1141 agccgaagtg gtcgtatcct gaaaataaaa aggcggacat ccgggaggtg ttttcctttc
     1201 ttattgaaaa cgggtttgtt gaaaaacagg cccgtttttt tcttctttca caataaactt
     1261 catgtttttc ccgtaaaaag atcaaacttt aactcatgaa gacatcctgg ttcatcaaaa
     1321 aagggatact gtttgttccg gtttctgtcg ccggggcatt cctttatctc ctgacgctgg
     1381 ctttctgcgc caccgtgttt ctcgccgtcg accggcattc ccattctgtg agcgacacgc
     1441 tgtacggtat tttcccgttt ttcgtctctg cttttacggt tcttttctgg atcgcttcga
     1501 acaccttgaa aaagagcggg gcctgatggc gttctacgat ctttcggcgg aagagcgaaa
     1561 aaggcacgtt gagaagatca gcagggaaat gcttgcggat atcgaaaaag gcgggacaaa
     1621 gaaaatcctc cgttactttt ccgacagcga tacctacatc aggaaatcag cttacatagc
     1681 tgccggaagg atttattccg cgcatcccgc tttgaggaaa aaaatcatcc gcctcctcgg
     1741 cgaattgctg caatcggatg atttttacat ccgccagaca gcagtcaatg cagccggtga
     1801 aattgcgatg aaagacttcg gtgctgtcga aaaattcttc gacaactcgc ttttcgatcc
     1861 gcatcattcc gtacgcaatg cggtgatcgg ctccatcaaa aaatccggcc ggaaaaatcc
     1921 gcggcccgtg ctggcctggg cgaagaaatt cctgcatcac ccggacaagg aagtgagacg
     1981 ggagatctgt cacggcattg agctgcgcgg acgtacgcac ccgcaggaga tccttcccct
     2041 cctggcggag ctccagttcg acacgaccgc acgcgtgaaa aacacgctcg ttcatgtgat
     2101 cggccagatc agctacaaaa aaaactgcct cccggttgtc attcccgcgc tcagcaaatg
     2161 gaagaacagg gagctggttg cgaaagcgat cgaagagatc atcgacgtgc acgaccgcta
     2221 ccggaatttt tcagcgctca cgcaaaaaga ggcgatcgat ttcattcaca aaaatctggt
     2281 gtaatttgcc ttgatgtttc caaccacatg aaaaagaacc tgcttcctgt catcgccgcc
     2341 gtccctttcc tcctgttcct ttgctgcagt tctcccccgg acaagtcagc agcgctgcag
     2401 gaacagatcg acagccttca gaaaaagctg gacgatacct acaggccggg cttcggtgag
     2461 ttcatgagcg gcatccaggt gcaccacgca aaactctggt tcgccggaaa agaacaaaac
     2521 tggaagctgg ccgacttcga gctgcacgag atcatggaga ctgtggacga tctgaaaaaa
     2581 taccagagcg aacgacggga atccgacaag ctcatcatgc tgga
//
LOCUS       DASTSF010000485         8589 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_532311,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000485 DASTSF010000000
VERSION     DASTSF010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 8589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8589
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_532311"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..898)
                     /gene="dnaN"
                     /locus_tag="VFU15_13975"
     CDS             complement(<1..898)
                     /gene="dnaN"
                     /locus_tag="VFU15_13975"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008616076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEU4718946.1"
                     /translation="MKFIVSSSSLLKQLQMLSGVLNSSNTLPILDNFLFEIDKGALTI
                     SASDLETTMTTHLTVESRDTGNVAVPAKLLIDTLKTFPEQPLTFTVDKKRFAIEIASD
                     YGKYKITGQNGDEFPKAPAVENSQSLDLDASVLGRAITKTLFAAGNDELRPVMSGVFV
                     QFSPESTTFVATDAHKLVRYRRTDIKATGSASFILPRKPLNLLKNVLASAEGIVRVEY
                     NATNARFSWGIAPDGTVGTTLTCRLIDGKYPNYEAVIPKNNPNKLTIDTNALLGSIRR
                     VSIFANKTTHQVRLKMAGSELNI"
     gene            complement(1171..2217)
                     /locus_tag="VFU15_13980"
     CDS             complement(1171..2217)
                     /locus_tag="VFU15_13980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718947.1"
                     /translation="MKRNTLTLIIALALGGITFWLIRMNSSSSIKKELRDFAVSDTAS
                     ITKIFLADKTMHQVTLTREKDGWKVDGKFDARPDAIEGLLGVIHDLSVREPVSIKSRP
                     FVIKQLATGAVKCEIYNGSDLVKVYYVGGDTPDDLGTYMLLADPETGENSSEPFIMEK
                     RGHNGYLTPYYSSNVIEWRDRSVFKYYAPDIRSIEVTNPRMPQTSFSVHQSANGVFGL
                     NSSTGQPLPFDTLMVKQFISYFGHISFEAFEKQLTPAQRDSVVHSTPAHIITVTDAAD
                     HKNVVKLFLKKNPNADSTTVTDPSQLYDPDRMYATVNNGQDFVVVQYYVFGKLLPVTD
                     YFLGQKVKEQNVVK"
     gene            complement(2385..4328)
                     /gene="gldG"
                     /locus_tag="VFU15_13985"
     CDS             complement(2385..4328)
                     /gene="gldG"
                     /locus_tag="VFU15_13985"
                     /inference="COORDINATES: protein motif:HMM:TIGR03521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     substrate-binding protein GldG"
                     /protein_id="HEU4718948.1"
                     /translation="MSSTNRNKRTDLIGLGLGIAILMLLNVASQYVFARFDLTSEKRY
                     TLTESTKEMLKKLDDIVYFKVYLDGDFPQGSGDYVHLRDETRIMLDEFRAYAGDKIQY
                     EFIDPGKNKDKKERIKYQKQLIDEGMIPKSEPFVDDDGNQVSMLLFPWAVASYHQKQT
                     LVPLLGFSTKKSDEESINKAVEELEYEMSNAIRKLQMQVKPKIAITQGHGEPDTTQLS
                     DFVKGLREYYDVDFVNFQGNLNAFRDTMNTAEQIRNKYTAVIIDKPDSMFTPKELFIL
                     DQFILYGGKALFLVDPVYASIDSLAQTGMTMCLPRTLGLEDLTFRYGARMNSNVVEDL
                     YCATVAIPVQGPQTQFMPAPWYYSPTILPTGQHPIVRNLDRIKFDFLSTVDTVETGTD
                     VKKTILLTTSDKSRYLRAPARVGLRNAMIQRDPRSFDKPDQPVAVLLEGTFDSYFRNK
                     FLPEELKNSKLIGYTDHSLHSSKIIVVGDGDVALNPVYKGKALPLGLDRMNRMNEEFY
                     ANKTFLLNCVNYLCDDKGLLSVRSREVTLRLLDRGKIREHKLKYQVMNTVYPVLGVML
                     FGIVLMWFRRRRYVQGKEPPEWLLKMGKYVLLLALYVVPLGILGVVMDNGMEFLFTAL
                     AVTVLLAAFYFVSYIRRNRMKGE"
     gene            complement(4348..5079)
                     /gene="gldF"
                     /locus_tag="VFU15_13990"
     CDS             complement(4348..5079)
                     /gene="gldF"
                     /locus_tag="VFU15_13990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009582029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     permease subunit GldF"
                     /protein_id="HEU4718949.1"
                     /translation="MYSLFIKEIRTFLSSLIGYIVIVVFLLGISLFMWVVKGQMNVLD
                     RGYANIDTLFNIAPVVFLFLIPAITMRSFADEKKSGTIELLFTRPLTDLQIILAKYFA
                     GFVLVLFSLLPTLYYFYCIYHLGVHTGNIDVGGTWGSYIGLLFLGSSFVAIGIFCSSL
                     TNSDIISFVYAFVLCYVCYLGFEQISSLSIFGKADVFIDALGIDSHYNSLSRGVIDTR
                     DVVYFLSFNAFFILLTKIRLESRKW"
     gene            5353..6669
                     /locus_tag="VFU15_13995"
     CDS             5353..6669
                     /locus_tag="VFU15_13995"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004289964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEU4718950.1"
                     /translation="MLVLKFGGTSVGSPERMKALVPLIVSSRPKIVVLSAMSGTTNSL
                     VAVAGALYAKENDKARDLIGKLEEKYRQVVDQLYSKEETRRKGQELVKEHFSYLGSFT
                     IDLFTANEEKAILAQGELLSTALMQFYLEETGVRSVLLPALNFMRIDENDEPDLDYIE
                     KHLQDELKKHPGETLFITQGYICRNAFGEIDNLKRGGSDYTATLIGAALRSEEVQIWT
                     DIDGMHNNDPRIVDKTFPIHELTFDEAAELAYFGAKILHPTCVLPAQKRNVPVALLNT
                     MQPQAKGTIIGGKVSGDRITAVAAKDGIAAIKIRSARMLLAYGFLRSVFEVFERYKTP
                     IDMITTSEVAVSLTIDNLKHLGEIVDELKTFGNVDVDREQTIVCIVGTFGKEKQGYAS
                     RVFDALRNIPIRMISYGGSENNISILIDTARKKEALAALNKGLFNL"
     gene            6686..7840
                     /gene="lysA"
                     /locus_tag="VFU15_14000"
     CDS             6686..7840
                     /gene="lysA"
                     /locus_tag="VFU15_14000"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015808148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEU4718951.1"
                     /translation="MFSQQRIAHLQPHRTPFYFYDLELLTRTLDAVKKASSKHGYHVH
                     YALKANVDRAVLQRIRSAGFGADCVSGNEVKRALETGFSPSQVVFAGVGKSDAEIRTG
                     LANDIFCFNCESLPEIMIISQLAREAGKKARIALRINPNVHANTHHYITTGLEENKFG
                     ISLHELPDVIDALAELPSLELAGLHFHIGSQITDLSVFRSLCMRVNEIQEWFASKRIP
                     LQHVNVGGGLGVNYHEPDKEAIPDFESYFGVFAKFLEVRHGQQVHFELGRALVAQCGS
                     LISRVLYVKKGVQTNFAILDAGMTELIRPALYQAYHKVENLTPSPGATVARYDVVGPV
                     CESSDCFGKAMDLPEVKRGDLIAIRTAGAYGEVMTSHYNLRELEETVYEG"
     gene            8192..>8589
                     /locus_tag="VFU15_14005"
     CDS             8192..>8589
                     /locus_tag="VFU15_14005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718952.1"
                     /translation="MTKILGLDLGTNSIGWAIRDTDALNENQIIDYGVLVFKKGVGDG
                     KSGEFSLAAERRKNRSKRRLYNAKRYRKWELLKVLIDNGMCPLTSDELRLWSIGNWQE
                     VHGKKKNLGRIYPISNEDFQRWLAFSPEYFG"
BASE COUNT         2002 a         2248 c         2402 g         1937 t
ORIGIN      
        1 aaatgttcag ctcgcttccc gccattttca ggcggacctg gtgcgtggtc ttattcgcga
       61 aaatggacac gcggcggata gagccgagca gcgcgttcgt atcgatggtg agcttgttcg
      121 ggttgttctt gggaataacg gcttcgtagt tggggtattt tccgtcgatg aggcggcagg
      181 tcaatgtcgt gcccaccgtt ccgtccggtg caattcccca cgagaaacgt gcgttggtgg
      241 cgttgtattc cacgcggacg atcccttccg cggaggcaag cacgttcttg agcaggttca
      301 gcggcttgcg gggaagaata aaggaagcgc tgccggtcgc tttgatgtcc gtgcggcggt
      361 aacgcaccag cttgtgcgcg tcggtagcca cgaacgtcgt gctctcgggt gagaattgta
      421 cgaacacgcc ggacatcaca ggacgaagtt catcgttgcc tgcggcgaaa agcgtcttcg
      481 tgatggcgcg tccgaggacg gatgcgtcga ggtcgagcga ctggctgttc tcgacggcag
      541 gagctttggg gaattcatcg ccgttctgtc ccgtgatctt gtattttccg taatcggaag
      601 cgatctcgat ggcgaaacgt tttttatcga cggtgaacgt cagcggctgt tccgggaagg
      661 ttttcagcgt atcgatgagc agctttgccg gcacggcaac gttgccggta tcgcgcgatt
      721 ccaccgtgag gtgcgtggtc atcgtggttt ccaggtcgga agccgaaatg gtgagcgctc
      781 ctttgtcgat ctcgaaaaga aaattatcaa ggataggcag cgtgttgctg gaattaagca
      841 cgccgctcag catctggagc tgtttcagca atgaggagct ggatacaatg aatttcataa
      901 atgagcagga taagccgtta gttacaaggc aaaattatcc cttttgacgg cctcagccgg
      961 gtcgcaaaaa ccaacttttg aactactttt taacagggga aagcaggtgc ttcccggtta
     1021 agtttccgtt cataaactct tcccgagaaa gggttttcca tgagactcag ggatttggaa
     1081 cgcagttttc gaaagctgct ccctaaatta aaacagcgcc ggcctctggt ccggaaaaac
     1141 agttcataca aaagccaaac ggctttccgc tcatttcacc acattctgct ctttcacctt
     1201 ctgcccgagg aaataatccg tcaccggaag cagcttgccg aacacgtagt attgcaccac
     1261 gacgaaatcc tgcccgttgt tgaccgtcgc atacatgcgg tcgggatcat aaagctgtga
     1321 aggatccgtg accgtggtgc tgtcggcgtt gggatttttt ttgaggaaga gcttcacgac
     1381 gttcttgtgg tctgccgcgt cggtcacggt gatgatatgt gccggtgtgg aatgcaccac
     1441 gctgtcgcgc tgcgcgggcg tcagctgttt ttcaaacgct tcgaagctga tgtggccgaa
     1501 gtacgagatg aactgcttca ccatcagcgt atcgaacgga agcggctggc cggtgctgct
     1561 gttcagaccg aaaacgccgt tcgcgctctg gtgaacgctg aaagaagttt gcggcatgcg
     1621 cgggttggtc acttcgatcg agcggatgtc cggtgcgtaa tatttgaaaa cgctgcggtc
     1681 gcgccactcg atgacgttgc tgctgtaata cggtgtgagg tagccgttat gcccgcgttt
     1741 ttccatgatg aacggctcgg aagaattttc gcctgtttcc gggtcggcca gcagcatgta
     1801 ggtgccgaga tcgtcgggcg tatcgccgcc gacgtaatac actttcacga ggtcgctgcc
     1861 gttgtagatc tcgcatttca ccgcgccggt ggcgagctgc ttgatcacaa agggcctcga
     1921 cttgatgctc accggctcgc gcacggaaag atcatggata acacccagca gcccttcgat
     1981 cgcatcggga cgcgcatcga attttccgtc tactttccag ccgtccttct cacgcgtgag
     2041 cgttacctgg tgcatggttt tatccgcaag aaaaattttg gtgatggagg cggtgtcgga
     2101 aacggcgaaa tcgcgcagct cttttttgat cgaggaagag ctgttcatcc ggatcagcca
     2161 gaaggtaatt ccgccgagag ccagggcgat gataagcgta agcgtgttgc gtttcataca
     2221 gggatcagtt cagagaaaac ggaaaagctt ccgcggtgat gcccggtgcg gatcttttcc
     2281 gggattccgg atgacagggg agttgtacag tgcaaatatc ggtaatgcga tccggcgcgg
     2341 aacatgaaaa acagtgagtg gaatggtttc gcgttcattc ggttttattc tcctttcatc
     2401 cggttgcggc ggatatagga tacgaaataa aaagctgcca gcaggaccgt gaccgccagg
     2461 gccgtgaaca ggaattccat cccgttatcc atcacaacgc cgagtatgcc cagcggcacc
     2521 acgtagagcg cgagcaggag aacgtatttc cccattttca gcagccattc cggcggttcc
     2581 ttgccctgca cgtaccttct ccgccggaac cacatcagca cgatgccgaa aagcatcacg
     2641 cccagcacgg gataaaccgt attcatcacc tggtatttca gcttgtgctc gcggatcttg
     2701 ccccggtcga gcaggcgcag cgtcacttcc ctcgaacgga cggagagcag gcccttgtcg
     2761 tcgcagaggt aattcacgca gttgagcagg aatgttttgt tcgcgtaaaa ctcttcattc
     2821 atcctgttca tgcggtcgag cccgagcggg agcgccttgc ctttgtaaac ggggttcagc
     2881 gccacatcac cgtcgccgac aacaatgatc ttcgagctgt gcagcgaatg atccgtgtag
     2941 ccgatgagct tgctgttctt cagctcctcg ggaaggaact tgttcctgaa atacgaatcg
     3001 aaggttcctt cgagcaggac ggccaccggc tgatcgggtt tgtcgaagct gcgcggatcg
     3061 cgctggatca tcgcattgcg caggcccacg cgtgcaggcg cgcgcaggta acgcgatttg
     3121 tcggacgtcg tcagcaggat cgttttcttc acatccgtgc cggtttcaac cgtatcgacc
     3181 gtgctgagaa aatcgaactt gatgcggtcg aggttgcgca cgatcgggtg ctgtcccgta
     3241 ggaagaatgg tgggcgaata ataccacggt gccggcatga actgcgtctg cggtccctgc
     3301 accggtatgg cgacggtcgc acagtaaaga tcttccacga cattgctgtt cattcttgcg
     3361 ccgtagcgga acgtaagatc ttccagtcca agcgtacgcg gaaggcacat cgtcatcccg
     3421 gtttgcgcaa ggctgtcgat cgaagcgtag accgggtcaa cgaggaacag cgctttcccc
     3481 ccgtaaagga tgaactggtc gaggatgaaa agttctttcg gcgtgaacat cgagtccggc
     3541 ttgtcgatga tgacggccgt gtatttgttg cggatctgct ccgccgtgtt catcgtatca
     3601 cggaacgcat tcaggttgcc ctggaaattg acgaaatcca cgtcgtaata ttcgcgcagc
     3661 cctttcacaa aatcgctgag ctgcgttgtg tccggttcgc cgtgtccctg cgtgatggcg
     3721 atcttcggct tcacctgcat ctgcagtttg cggatcgcat tcgacatttc atattccagc
     3781 tcttccaccg ctttgttgat ggattcttcg tctgattttt tcgtggagaa tcccagcagc
     3841 gggaccagcg tctgcttctg gtggtaggaa gccacggccc acgggaacag cagcatgctc
     3901 acctggttgc cgtcgtcgtc gacgaaaggc tccgacttcg ggatcatccc ctcgtcgatg
     3961 agctgcttct ggtatttgat gcgttctttt ttgtccttgt tcttgccggg atcgatgaat
     4021 tcgtactgta tcttgtcgcc tgcatacgcg cggaactcat ccagcatgat cctcgtttca
     4081 tcgcggaggt gaacataatc gccggaaccc tgcggaaaat cgccgtcaag atacaccttg
     4141 aagtagacga tgtcgtcgag cttcttcagc atctctttcg tgctttccgt cagcgtgtat
     4201 cttttttcag aggtaaggtc gaaacgcgca aagacgtatt gcgaagccac gttcagcagc
     4261 atgagaatgg caatgccgag cccgaggccg atcagatctg tccgtttatt gcggttggtg
     4321 ctgctcaatt gcgtggatca aaaaaagtta ccatttgcgg ctttcgaggc ggatcttcgt
     4381 cagcaggatg aagaacgcgt tgaacgaaag aaaatacacg acatccctcg tgtcgatgac
     4441 gcccctgctg agtgaattgt aatgcgagtc gatgcccagc gcgtcgatga atacgtcggc
     4501 tttcccgaaa atggacagcg agctgatctg ctcgaagccg aggtagcaca cgtaacaaag
     4561 cacgaacgcg taaacgaagg aaatgatgtc gctgttcgtg agcgaggagc agaagatccc
     4621 gatcgcgacg aacgaagagc cgaggaaaag cagcccgatg tacgatcccc acgttccgcc
     4681 cacgtcgatg ttgcccgtgt gaacgccgag gtggtaaatg cagtagaagt aatagagcgt
     4741 gggaagaagc gagaacagca cgagcacgaa tccggcgaaa tatttcgcga ggatgatctg
     4801 caggtcggtc agcgggcggg taaacagcag ctcgatagtg ccggattttt tttcatccgc
     4861 gaacgaacgc atcgtgatcg caggaataag gaacaggaaa accacgggcg caatgttgaa
     4921 gagcgtatcg atgttcgcgt agccgcggtc gagaacgttc atctgcccct tcaccaccca
     4981 catgaacagg ctgatgccga gcaggaacac cacgatcacg atgtacccga tcagggagct
     5041 gaggaacgtg cggatttctt taatgaagag tgaatacata aataaaccgg gggcggtttc
     5101 gcgctttcag aataacagtg tgcgctcaaa aataacgaat gaatcggctg cctgatttct
     5161 tccgaaggga accttttaac atccttttgc acaatcttcc tctttcgggt tttcagttcc
     5221 gtgttccgga ccggcagata agcgacaaac tttgcgacct ggcggaaaat agcagcccgg
     5281 aactcaaaac ccggcgccgg aaaccgctaa cccgcgcagt tttttatctt cgcaagcctt
     5341 taaccattca ttatgcttgt cctgaaattc ggtggcactt ccgtgggcag cccggaaaga
     5401 atgaaagccc ttgtgccgct catcgtcagc agccggccga agatcgtcgt gctgtctgcc
     5461 atgtccggta cgacgaacag cctcgtcgct gttgccggtg cgctttatgc gaaagaaaac
     5521 gataaagccc gcgacctgat cgggaaactg gaagaaaaat accggcaggt ggtggatcag
     5581 ctgtactcga aagaggaaac gcgcaggaaa ggacaggagc tcgtgaaaga acatttcagc
     5641 tacctcggca gcttcaccat cgatcttttc accgccaacg aggaaaaagc catcctcgcg
     5701 cagggcgaat tgctcagcac ggcgctgatg cagttttacc tcgaagagac aggcgtccgc
     5761 tcagtgctgc tccccgcgct gaatttcatg cgcatcgacg agaacgacga gcccgacctg
     5821 gattatattg aaaagcacct gcaggacgag ctgaaaaagc acccgggcga aactcttttc
     5881 atcacgcagg gctacatctg ccgcaacgcg ttcggcgaga tcgacaacct gaagcgcggc
     5941 ggtagcgatt acaccgcaac gctcatcggt gctgcgctgc gttcggaaga agtgcagatc
     6001 tggaccgaca tcgacggcat gcacaacaac gatccccgca ttgtcgataa gacatttccc
     6061 atccacgagc tcacgttcga tgaagccgcc gagctcgcgt atttcggcgc aaagatcctt
     6121 cacccgacgt gcgtgctgcc cgcgcagaag cgcaatgtgc ccgtcgcgct gctcaacacc
     6181 atgcagccgc aggcgaaagg aaccatcatc ggcggaaaag tttccggcga ccgcatcacg
     6241 gccgtagcgg cgaaagacgg catcgccgcg atcaagatcc gttcggcgcg gatgctgctt
     6301 gcttacggtt tcctgcgctc ggtcttcgaa gtgttcgaac gctacaaaac gccgatcgac
     6361 atgatcacga cgtcggaggt ggccgtgtcg ctcaccatcg ataacctgaa gcacctcggc
     6421 gagatcgtgg acgaactgaa aacgttcggc aacgtcgacg tcgaccgcga gcagacgatc
     6481 gtctgcatcg tgggcacatt cgggaaagag aagcagggct atgcttcccg cgttttcgac
     6541 gcgctcagga atattcccat ccgcatgatc tcgtatggcg gcagcgagaa caacatttcc
     6601 atcctcatcg acaccgcgcg caaaaaggaa gcgctggcgg cgctgaacaa aggactcttc
     6661 aatctctgaa cgaaaaaacg cacgcatgtt cagccagcag cgcatagcac atcttcagcc
     6721 gcaccggacg ccgttctact tctacgacct ggagctgctc acacgcacgc tcgacgcggt
     6781 gaaaaaagcc tcgtcgaagc acggctacca cgtgcattac gcgctgaagg cgaacgtcga
     6841 ccgcgcagtc ctgcagcgca tccgcagcgc gggcttcggc gccgactgcg tgagcgggaa
     6901 tgaagtgaaa cgcgcgctgg aaaccggttt ttctccttcg caggtcgttt ttgccggagt
     6961 agggaagagc gatgccgaga tcaggaccgg cctggcgaat gatattttct gcttcaactg
     7021 cgaatcgctg cccgagatca tgatcatttc ccagctcgcg cgcgaagccg ggaagaaagc
     7081 gcgcattgcg ctgcgcatca acccgaacgt gcacgcgaac acgcatcact acatcacgac
     7141 gggactcgaa gaaaataaat tcggcatcag cctgcacgaa ttgcccgacg tcatcgatgc
     7201 gctcgcagag cttccttcgc tggagctggc cgggctgcat tttcacatcg gctcgcagat
     7261 caccgatctt tccgttttcc gttcgctgtg catgcgcgtg aacgagatac aggaatggtt
     7321 cgcctcgaaa cgcattccgc tgcagcacgt caacgtcggc ggcggactcg gcgtgaatta
     7381 ccacgagccg gacaaagaag ccatcccgga ttttgaaagc tatttcggcg tcttcgcgaa
     7441 atttctcgaa gtgcgccacg ggcagcaggt ccatttcgag ctgggacgcg cgctggtagc
     7501 acaatgcggt tcgctcatct cgcgcgtgct gtatgtgaag aaaggcgtgc agacgaattt
     7561 cgccatcctc gacgcgggaa tgacggagct gatccgcccc gcgctgtacc aggcttacca
     7621 caaagtggaa aacctcacgc catcgcccgg cgcaaccgtt gcgcgctacg acgtcgtcgg
     7681 tccggtgtgt gaatcgtccg actgtttcgg caaagcgatg gatcttcccg aagtgaaacg
     7741 cggcgacctc atcgccatcc gcacggccgg cgcctacggc gaagtgatga ccagtcacta
     7801 taatctgcgg gagctggaag agacggtgta tgaaggatga atgtggagtt ctgaatttta
     7861 aattgcgggt agtcttactt tagaacagac gctttttcct cgcctttcga taataatctg
     7921 tgccgggaca ggatactttg gtattctgag gatggtttat tgactttttg taaaaaacgg
     7981 ggtagcttgg ccaataaaaa agacgacccc ggttgttacc agggccgcct tagatgtttt
     8041 cacaacggaa taatccttat tgtgaaataa tttttttgta aaaaataagc accacagaag
     8101 tatgcggtgc tcaagagcaa ttcgctcttc ggcttatagc cttgttgtga attgcattac
     8161 aagaataaca aaaaacacta tacaaaaaaa tatgacaaaa attttaggcc ttgatttagg
     8221 taccaattct attggatggg caattcggga tactgatgcc ttaaatgaaa accagatcat
     8281 agattatgga gtccttgttt ttaaaaaagg tgtaggggac ggtaaatcag gggaattttc
     8341 cctcgcggca gagcgaagaa agaacaggag caaacgaagg ctttataatg caaaaaggta
     8401 tcgtaaatgg gaactgctga aagtcttgat tgataacggc atgtgcccac ttacatcgga
     8461 cgagttgcgt ttatggagta ttgggaactg gcaagaagtg catggtaaaa agaagaacct
     8521 tggccgtatc tatcctataa gcaacgaaga ctttcagaga tggctggcat tttcccctga
     8581 atacttcgg
//
LOCUS       DASTSF010000486         2097 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1248948,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000486 DASTSF010000000
VERSION     DASTSF010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2097
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1248948"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /locus_tag="VFU15_14010"
     CDS             <1..238
                     /locus_tag="VFU15_14010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718953.1"
                     /translation="EYTNEVIDEEQHERIDSTSDVYGDGKTHDEILTTIRTAYENRFL
                     GEGKKITYVSFRLKKNYGESVRVFPFNGAAAEKE"
     gene            516..1673
                     /locus_tag="VFU15_14015"
     CDS             516..1673
                     /locus_tag="VFU15_14015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718954.1"
                     /translation="MKTTFACLTLTAGLFFFAGTMKAQQDTTKKAEFKPSGKVWGYAF
                     GDYYFKTHADSANRGNSQYSNMPVNANSFELRRVYLGYDYNISERFSSEFLLSYEGQT
                     GSDGSRTTFVKAANLRWKNLYKGADLIFGLQATPAFPMMSEKIWGYRSDEKTILDMRK
                     LSGSADLGIGLQGKCGSDGQFGYDFLVANGSGTKLEGDIFKKFYGDVYAKFMDKKLIV
                     DLYGDFERTNMTPDFHKYRNTGKLTLAWNTDALTIGAEAFLQIQHNYVGFVDTSMSPV
                     HTDTTDAKAMGVSIFVRGTIIKDKLAFFARYDMYNPDLGYKKDYIYTMGGAPVTESFA
                     TAGIDFTPAKNVHIMPNVWFNSYMSRAKGASGKVKSDYDFVPRLTFWYIFK"
     gene            1782..>2097
                     /locus_tag="VFU15_14020"
     CDS             1782..>2097
                     /locus_tag="VFU15_14020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU4718955.1"
                     /translation="MKYTKAIASLGITAALILSSCGSGGEKNGTDSTGNNATVSEILG
                     AGATFPYPLYSKMFDEYNKKTGIKINYQSIGSGGGIKGLHDKTVDFGASDAPMSDDDL
                     SKS"
BASE COUNT          590 a          572 c          481 g          454 t
ORIGIN      
        1 tgaatacacc aatgaagtga tcgacgaaga gcagcacgag cgcatcgatt ccacgagcga
       61 cgtttacggc gacgggaaaa cgcacgatga aatactcacc accatccgca ctgcgtacga
      121 gaaccgtttc ctcggcgaag gaaaaaagat cacgtatgtt tcttttcgcc tgaaaaaaaa
      181 ttacggggaa tccgtgcgcg tctttccgtt caatggcgct gccgcggaaa aagaataaaa
      241 tttccgcatc acaatttctt cacaggcggc catcatccct cccggaactg ttttttctta
      301 acagtaacga aacacacttg caggtattcg tcagcctgga aatcaatgcg ttattcattt
      361 tcaattaacc tatatgaccc tatttattaa caataaaggg gataaactgg agcaaaaagg
      421 cgtcgttaac acacaattaa tattggcgta attcatcagt aactatccgc aggttctttt
      481 gcacgtaaaa ttaatttaaa caaaaagaga cctgaatgaa aacaacattc gcctgcctga
      541 cccttactgc gggactgttc tttttcgccg gaacgatgaa agcccagcag gacaccacta
      601 aaaaagcaga attcaagcct tccgggaaag tctggggcta tgccttcggc gattattatt
      661 tcaaaacgca cgccgattcc gccaaccgcg gaaactcgca gtattccaac atgccggtga
      721 acgcgaattc cttcgagctg cgccgtgtgt acctcggtta tgactacaac atcagcgagc
      781 gcttttcctc tgaattcctg ctttcctacg aaggacaaac cggcagcgac ggctcgcgca
      841 cgacattcgt caaagcggcc aacctccgct ggaagaacct ctacaaaggc gctgatctta
      901 ttttcggtct ccaggcgacg ccggcgttcc ccatgatgtc ggaaaaaata tggggctacc
      961 gctccgatga aaagaccatc ctcgatatgc gcaagctgag cggttccgcc gacctcggta
     1021 tcggcctcca gggcaaatgc ggcagcgacg ggcagttcgg ctacgatttt ctcgtggcga
     1081 acggatcggg caccaagctc gaaggcgaca tcttcaagaa attttacggc gatgtttacg
     1141 caaagttcat ggacaagaaa cttattgttg acctgtacgg cgatttcgaa cgcacgaaca
     1201 tgacgcctga cttccacaaa taccgcaaca cgggcaagct gacgctcgcc tggaacacgg
     1261 atgcgctcac catcggtgcg gaagcgttcc tgcagatcca gcacaattac gtgggcttcg
     1321 tcgatacgtc catgtcgcct gtgcacacgg acacgacgga cgcaaaagcc atgggcgttt
     1381 ccatcttcgt ccgcggaacg atcatcaagg acaagctcgc tttcttcgca cgttacgaca
     1441 tgtacaatcc tgacctcggc tacaagaaag attatatcta cacgatgggc ggagctcccg
     1501 tgaccgaatc attcgcaacg gccggcatcg atttcacgcc tgcgaaaaat gtgcacatca
     1561 tgccgaacgt atggttcaac agctacatga gccgcgccaa aggcgcttca ggcaaggtga
     1621 agagcgatta tgacttcgtt ccccgcctca cgttctggta catcttcaaa taaatagttt
     1681 atacaaagct catgctcgca tagcgttttc ttaacaatgc tgaacgagtt ttgcagcgcc
     1741 tgacacgaaa aaaatctcta aataataaag aaaaaacaat catgaaatac acaaaagcca
     1801 ttgcatcact cgggatcacg gcagcgctga tcctctcttc ctgcgggtca ggcggagaaa
     1861 agaacggtac ggattcgaca ggaaacaacg ccaccgtgag cgaaatcctc ggggcgggag
     1921 cgaccttccc ttatccgctg tattccaaaa tgttcgacga atacaacaag aaaaccggca
     1981 tcaagatcaa ctaccagtcc atcggttccg gcggcggcat caaaggtctg cacgataaaa
     2041 ccgtcgattt cggcgcttcc gacgccccga tgagcgatga cgatctctcc aaatcac
//
LOCUS       DASTSF010000487         7815 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_467234,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000487 DASTSF010000000
VERSION     DASTSF010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 7815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7815
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_467234"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1462)
                     /locus_tag="VFU15_14025"
     CDS             complement(<1..1462)
                     /locus_tag="VFU15_14025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718956.1"
                     /translation="MPQRHREYLYSKFKNGEIPNQDDFDDSFDSALNLLDDGITISKK
                     GDQKHFGINTDPTCLLSIRGDQVNVDSLICLQSTDHSQQWNINLNPTKGKTPGFSISN
                     ASKGNATSNLFIDAATGNVGINSVTPTEKLSVFGSVDGGYVSALITNLQSGLDGWALS
                     VVDDNVTPYRVGAFGLVEKDGSTGTERITVLPAITTPSNTYYRTGINTQLPYSTLHVD
                     RSEADAETDISLNINSGIILSGSMIGHHLGFDNHQIQARVGDYVAGSTTIELNTSVLN
                     IQPLGGGVVINNGLPAAQQLKIDDNGNVGMGAAPSAERLVVAGAVTFGDSVSDTPAQG
                     TVRWSPGSAVEPADLQVYKDDAWQSLTTHFVSDGLWTDGGSGTIYYDPASGIPRVGIG
                     TTTPASLLTVNETNASSTSNAAADIITSATTSSSDVAVSRFGLRVNSTGPWSTNAAAN
                     NIGIFVENVSGQTNVNANLAAVLNGSTVIGHLTGLPL"
     gene            complement(1484..4987)
                     /locus_tag="VFU15_14030"
     CDS             complement(1484..4987)
                     /locus_tag="VFU15_14030"
                     /inference="COORDINATES: protein motif:HMM:NF016736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HEU4718957.1"
                     /translation="MSDTCNNKNPLSRDGTSQAQRLLNALLPSYVLVDERSLDDLVAF
                     VQKFATQVNYFNLADAVNGDWVGFFTKTISDEQRTEPHYALLISFLEIFKIAQDDLNT
                     ITQRHLDFYYHEVLNLQEKPAVPDQVYVIFKLAQQVSDALVPKGKELNAKKDATGIQL
                     VYDTDKDIVVNKGVVEELKAVFINRPIDPAASPQEFPDLIPVPDPAQPVSLFQRNEWR
                     IYSSPVANSSDGIGGDLTGDEKRWRIFGRPQPVSDGSSVADRPQADVGFAFASPLLFL
                     AEGTRTVTIRINSQRAVRPSPVAVALQAPTSIATSYATHSLSAQLRSVNPSLHASIIA
                     NFVINQQNNALKGAISVYFSGEKGWIAPEIETNTYFDTNGDLVIERTLTKGQKPVVAY
                     DQSVLLQPFNTQWPVVKIMLNPDHAASPYIYEQLSPKNIYTADLTVDVVDVKNVIVQS
                     EDTVLATEKPFTPFGSQPVVGSTFYIGSNEIFQKKLSELDVAITWHGLPDVSRAPEGF
                     VDYYNYYLPEGENLKRQTLSFKVDAALIDKQSWHSLNTSNNGYGLFQPKTQLVGTVYR
                     HPVDEDITLTLNTSFLAGVPRDPYMDPVEEFTATTKKGFLRLTLADVDFGHGIFQNSY
                     AKQAIELALQHSGANLPNEPYTPTIKEISVNYRSTERVNLTRQNDDAVNEANYKNRVE
                     QFFHVLPFGVAENHPFITKQTKNINLLPQFRDEGELFIGVSGLQTPETLSILFKVAEG
                     SADPDLEKQKVNWSYLYNNEWYDFTQLQILSDGTNGLLTSGIVTFDLPKAFNNTNTAL
                     PDNLFWIRVSVEHDSAAVCDLVDVIAQAVTATFTDNGNDPDHLRTPLPANTISDFINS
                     DAAIDKISQPYSSFGGHIKEQSNEFYRRVSERLRHKDRAVTPWDYEHLVLEQFPTLYK
                     VKCLNHTRYSSLADINELVAGHVTLVIISNLLNKNAVDPLQPKTSLITLEEIIEYVDT
                     IKPPAAELHVRNPIFEEIRVEFNVRFLPGYDNGFYGKQLNEEIRQFLAPWAYGTKDIA
                     FGGAIHKSMIINFVEERPYVDYVTCFNMDQVVPVGKNLPPTILKNVETAETTTSASVL
                     TSYPQHSITVLETDECACDDNLVLSPVPNPDQPCDDCGDNKEKIATGIGADEIDSTFI
                     VGHAPRAGIDFWVLEKDFIVQ"
     gene            complement(4984..5424)
                     /locus_tag="VFU15_14035"
     CDS             complement(4984..5424)
                     /locus_tag="VFU15_14035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020598758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HEU4718958.1"
                     /translation="MASEQTLKEIFDTGGFLGQGWSFPPTFTKGTNTVELVQAEEDIK
                     ESLHIILSTTLGERVMLSDFGANLDQQVFEPMDAAFQPFVTSIIRRAINIYEPRVNLD
                     SVDVASEVLEGKIELTINFTIIANNTRSNIVYPYYLTEGTNVQQ"
     gene            complement(5438..5716)
                     /locus_tag="VFU15_14040"
     CDS             complement(5438..5716)
                     /locus_tag="VFU15_14040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012648813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HEU4718959.1"
                     /translation="MPAAAKVGDTTTHGGTIVGPGVATVLIKGKPAAVAGDNHVCPIP
                     PNTGHITSSPFPMGSTTVLINNKPALRAGDTCVCGAASAIGEPTVMIG"
     gene            complement(5729..7552)
                     /gene="vgrG"
                     /locus_tag="VFU15_14045"
     CDS             complement(5729..7552)
                     /gene="vgrG"
                     /locus_tag="VFU15_14045"
                     /inference="COORDINATES: protein motif:HMM:TIGR01646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HEU4718960.1"
                     /translation="MPKINSTQDTIPGHKKISHPVYTVTIGGKKVSSNPGVASVMVNK
                     GINRISVAEIVLYDGSASKQEFKLSEGNDYLPGKEVIIEATYQTQEDTSTQQIFSGII
                     IRQSIKANLTGPSTLLLEARDKIIKSTIERKNACYFDKKDSEIIEQLLGTYGVQKEVA
                     ATKVTHKDMVQYYSTDWDFLVSRAEANGLLVYVDDGKVTVADPEKAQATSPSLEITYG
                     TNIVEIDAEMDARDQYAGVTSKDWDFTKQELGVNIPSTPVFKEEGTLNSLGLSDVMGS
                     INYRLQHSGKLEEGELTAWADAKFLRSKMTKIKGRVRIYGTNVAKPGKTFTFDGLGDH
                     FNNTAFISSVTHSFSAHSTWYTDIQFGYSDEWFYERHDNITEKSAAGLLPAMHGLQPG
                     KITAVKDTDSGDFRVRVKYPLIESDSKGIWARWATLDAGNNRGFVNRPYEGDEVILGF
                     VNNDPRDPVVLGMLHSSVNAIPSDLTPDDNSLNKGWVTKNNIKILLHDEADKDKVTIE
                     TPGGNMIVIDDEKKSITITNKGDTSVTNTITMDKNGIKLESDKDIIINAKGSSSKITA
                     TGSQNVALESKQGKFTAKGQTGAEMTTSGQAVLKGQLVKIN"
     gene            complement(7554..>7815)
                     /locus_tag="VFU15_14050"
     CDS             complement(7554..>7815)
                     /locus_tag="VFU15_14050"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HEU4718961.1"
                     /translation="NPLRATLSASFIQVPFAGNDRASLSWQSPDITKLHLVTAGDTIY
                     NIARNEYGSESYYLQIAEANDLKNYRRLVPGTQLILPPVKTV"
BASE COUNT         1624 a         1937 c         2177 g         2077 t
ORIGIN      
        1 tgagcggaag tcccgtcagg tgaccgatca ccgtgctgcc gttgagtacg gcggcgagat
       61 tggcgttgac gttcgtctgc ccggaaacgt tctcgacgaa aatgccgatg ttgttcgcgg
      121 ctgcattcgt gctccacgga ccggtgctgt tcacgcgcaa tccgaagcgg ctcaccgcta
      181 cgtcgctgga cgaagtggta gcggaagtga tgatgtcggc tgcggcattc gatgtagaag
      241 acgcattggt ttcattgacc gtgagcaggc ttgcaggtgt cgtggtgccg ataccgaccc
      301 tgggaattcc gcttgccgga tcgtagtaaa tcgttccgct tcctccgtcg gtccagagtc
      361 cgtcgctgac aaagtgcgtg gtaagcgatt gccatgcatc gtctttgtag acctgcaggt
      421 cggccggttc caccgcgctg ccggggctcc agcgaactgt tccctgcgcg ggcgtatcgg
      481 aaactgaatc tccgaacgta acggctcctg caaccacgag gcgttccgcc gaaggcgctg
      541 cgcccatgcc gacatttccg ttgtcgtcga tcttcagctg ctgtgcggcg ggaagaccat
      601 tgttgatcac aacaccgcca ccgagcggct gtatgttgag cacggatgta ttcagctcga
      661 tagtggtgct gccggcaacg tagtcgccga cgcgcgcctg tatctggtgg ttgtcgaacc
      721 cgaggtggtg cccgatcatc gagccggaca ggatgatgcc ggaatttatg ttgagggaaa
      781 tatcagtttc agcatccgct tccgaacgat cgacatgcag cgttgaatac ggaagctgcg
      841 tgttgatgcc ggtccggtaa taagtattgg acggagttgt gatcgccggc aaaaccgtaa
      901 tgcgttcggt tcccgttgag ccgtcttttt ccaccaggcc gaatgcgcct acgcgatagg
      961 gcgtgacgtt gtcgtcaaca acggacagtg cccatccgtc gagcccgctc tgcaggttgg
     1021 tgatgagcgc tgaaacataa cctccgtcca cgctgccgaa gacggaaagc ttttccgtcg
     1081 gtgtaacaga gttgatgccg acattcccgg ttgctgcatc gatgaagagg ttgctggtgg
     1141 cattgccctt ggaagcattg ctgatgctga agcccggcgt cttccctttc gtagggttga
     1201 ggttgatgtt ccactgctgc gaatgatcgg tgctttggag acagatcagc gagtcgacat
     1261 tcacctggtc accccggatg gagagcaggc aggtcggatc ggtattgatg ccgaaatgtt
     1321 tttggtcgcc cttcttggat atggttattc cgtcatcgag aagattgagc gccgaatcga
     1381 atgaatcgtc aaaatcgtcc tggttcggaa tttctccgtt cttgaatttc gagtataagt
     1441 actctctgtg tctttgcggc atggcgataa tttgttttgt attttattgt acgataaaat
     1501 ctttttcgag gacccagaag tcgatgcccg cacgcggtgc gtgacccacg atgaacgtgg
     1561 agtcgatctc gtcggcgccg ataccggtcg caattttttc tttgttgtct ccgcaatcgt
     1621 cgcagggctg atcggggttc ggaacaggcg agaggacaag gttgtcgtcg catgcacatt
     1681 catcagtctc gagcacggtg atcgaatgct gcgggtaaga cgtaaggacg gaagcggacg
     1741 ttgtggtttc tgccgtttca acgttcttca ggatcgtggg cggaaggttt ttccccacag
     1801 ggaccacctg gtccatgttg aagcaggtca cataatccac gtacggacgt tcttccacga
     1861 aattgatgat catcgatttg tggatggcgc cgccgaacgc gatatctttt gttccgtacg
     1921 cccacggcgc gaggaactgc ctgatctctt cgttgagctg ttttccgtag aagccgttgt
     1981 cgtaaccggg aaggaaacgc acgttgaatt ccacgcgtat ctcttcgaat atcgggttgc
     2041 gcacatgcag ctctgcggcc ggtggcttga tcgtgtcgac gtattcgatg atctcttcga
     2101 gcgtgatcag gctggttttc ggctgcagcg gatcgactgc gttcttgttg agcaggttgg
     2161 aaatgatcac gagcgttaca tgtcccgcta ccagttcgtt gatgtcagcc agtgagctgt
     2221 aacgcgtgtg attgaggcat ttcactttat atagcgtcgg gaactgctcg agcacaagat
     2281 gctcgtaatc ccatggcgtg acggcacggt ccttatgacg caggcgctca cttacgcggc
     2341 ggtagaattc attactctgc tctttgatgt gcccgccgaa ggaagagtag ggctggctga
     2401 tcttatcaat ggctgcgtcc gaattgataa aatcgctgat cgtattggcc ggcagcggcg
     2461 tgcgcaggtg gtcgggatcg ttcccgttat cagtgaatgt tgctgtaacc gcctgtgcga
     2521 tgacatccac gagatcgcat acggcagccg agtcgtgctc gacggaaacg cgtatccaga
     2581 agaggttatc cggcaacgcc gtatttgtgt tgttgaatgc tttcggaaga tcgaacgtga
     2641 cgatccccga agtaagcagg ccgtttgttc cgtcggaaag gatctgcagc tgcgtgaaat
     2701 cgtaccactc gttgttgtac aggtagctcc agttcacttt ctgcttttca agatccgggt
     2761 cggcgcttcc ttcggcgact ttgaaaagaa tggataatgt ttcgggcgtt tgcagtccgc
     2821 ttacgccgat gaacaattca ccttcatccc ggaattgcgg aagcaggttg atgttcttcg
     2881 tctgcttcgt aatgaacgga tgattctccg caacaccgaa cggaagcacg tggaagaact
     2941 gctcgacgcg gtttttgtaa ttcgcttcgt ttactgcgtc atcgttctgc cgggtgaggt
     3001 tcacacgttc cgtcgacctg taattgaccg aaatttcctt gatggtcggt gtgtacggtt
     3061 cgttgggaag gttcgctccg ctgtgttgaa gggcaagctc gatcgcctgc tttgcatacg
     3121 agttctggaa aatcccgtga ccaaaatcca catcggcgag tgtaagccgc aggaatcctt
     3181 ttttcgttgt cgctgtaaat tcttccacgg gatccatgta aggatcgcgg ggcacgccgg
     3241 caaggaaaga cgtgttaagc gtgagcgtga tgtcttcgtc aacgggatga cggtaaacag
     3301 taccgacaag ctgcgttttc ggctggaaga gaccgtaacc gttattcgaa gtattcagcg
     3361 aatgccagga ctgcttgtcg atcagggcgg catccacttt gaaactcaaa gtttgccgct
     3421 tgaggttctc gccttcaggc aggtaatagt tgtaataatc gacgaatcct tccggtgcgc
     3481 gcgaaacatc aggaagcccg tgccacgtga tggcgacatc gagctcgctc agttttttct
     3541 ggaaaatttc attgctgccg atgtagaaag tggaacctac aaccggctgg cttccgaaag
     3601 gcgtgaacgg cttttcggta gcgagcaccg tgtcttcact ctgtacgatc acgtttttca
     3661 catcgaccac atcgacggtg agatcagcgg tgtaaatatt tttcgggctc agctgctcgt
     3721 agatgtacgg cgaagcagca tgatcgggat taagcatgat cttcaccacc ggccactgcg
     3781 tgttgaacgg ctgcagcaat accgactggt cataagcgac gacgggtttc tgacctttcg
     3841 tgagcgtgcg ctcaatgaca aggtcgccgt tcgtgtcgaa atacgtgttc gtttctattt
     3901 caggagcgat ccatcctttt tctccgctga aataaacgct gatcgcacct ttcagcgcat
     3961 tgttctgctg gttgatgacg aagttcgcga tgatcgacgc atgcagggaa ggattaacac
     4021 ttctcagctg cgccgagagt gaatgcgtag cgtatgacgt agcgatcgat gtgggcgcct
     4081 gcagcgccac agcgaccggg ctcggccgga ccgcgcgctg cgagttgatg cgtatcgtca
     4141 ctgtgcgtgt tccttcggca aggaagagca ggggagaggc gaaagcaaaa cctacatctg
     4201 cctgcgggcg gtcggccacg ctgcttccat cggaaacagg ctgcggtctt ccgaaaatgc
     4261 gccagcgttt ttcatcgccg gtgaggtctc cgccaatgcc gtcggaagaa ttcgcaacgg
     4321 gcgaagaata gatgcgccac tcgttgcgct ggaaaagcga gaccggctgc gcgggatcgg
     4381 gaacgggaat aagatcaggg aattcctggg gtgatgcagc aggatcaatg ggccggttaa
     4441 tgaaaaccgc cttcagctct tcgacaactc ctttgttgac gacgatatcc ttatccgtat
     4501 cataaacaag ctggatgccg gttgcatctt ttttggcgtt gagctctttt cctttcggaa
     4561 ccagtgcatc ggaaacctgc tgcgccagct tgaaaatgac atacacctgg tcgggaaccg
     4621 caggtttttc ctgcaggttc agcacttcat ggtagtagaa atcaagatga cgctgggtga
     4681 tcgtgttcag gtcgtcctgt gcaattttaa atatttcaag aaaggagatc agcagtgcat
     4741 aatgaggttc ggtacgctgc tcgtcggaaa tcgttttggt gaagaaccca acccagtcgc
     4801 cattgacggc atcagccagg ttgaaataat tgacctgggt agcgaatttc tgaacgaatg
     4861 caacaagatc atccaggctg cgctcgtcca ccagcacgta tgaaggaagg agagcattga
     4921 gcaggcgctg cgcctggctg gtgccatccc tggaaagcgg atttttatta ttgcaggtat
     4981 cgctcattgt tgaacgtttg ttccctcggt gaggtaatac ggataaacaa tattggatcg
     5041 cgtgttgttc gcgatgatgg taaagttgat ggtgagctcg atcttacctt caagcacctc
     5101 gctcgctaca tcaacgctgt cgagatttac tctcggttcg taaatattga tcgctcgtct
     5161 tatgatggat gtgacaaaag gctggaacgc agcatccata ggttcgaaca cctgctggtc
     5221 gagattggcg ccgaaatcgg aaagcataac acgctctccg agcgttgtgg aaagaataat
     5281 gtgaagactt tccttgatgt cttcttccgc ctgcacgagt tccacggtat tggttccttt
     5341 tgtgaacgtg ggcgggaacg accaaccctg gccgagaaaa ccgcccgtgt cgaaaatttc
     5401 tttgagtgtc tgttcgcttg ccataatctg aaatgcttta accgatcatt accgttggtt
     5461 cgccaattgc cgatgcagct ccgcacacac aggtgtcgcc tgcacgaagc gccggcttgt
     5521 tattgatcag caccgtggtg cttcccatgg gaaacggtga agacgtaatg tgtcccgtat
     5581 tcggtggtat aggacacacg tgattgtctc ctgccacggc cgccggtttt cctttgatga
     5641 ggaccgtcgc cacaccgggt cccacaatcg ttcccccgtg tgttgtcgta tctccaactt
     5701 ttgctgctgc cggcatgagt ttgttcgttt aattgatttt gaccagttgt cctttaagaa
     5761 cggcctgccc gcttgttgtc atttccgccc cggtctgtcc ttttgctgtg aattttccct
     5821 gcttggattc cagcgccaca ttctggctgc cggtagccgt gatcttactg gaagaacctt
     5881 tcgcattgat gataatatct ttatcgcttt ccaatttgat cccgttcttg tccatcgtga
     5941 tggtgttggt cacgcttgta tcgcccttat tggtaatcgt gattgatttt ttttcatcgt
     6001 cgatcacgat catgttccct cccggagttt cgatggtgac cttgtctttg tctgcctcat
     6061 cgtgcaacag gatcttgatg ttgtttttcg tcacccatcc tttattgaga ctattgtcgt
     6121 cgggtgtcag gtcggaggga attgcgttca cgctgctgtg cagcatgcca agaacaaccg
     6181 gatctctcgg atcattgttg acaaagccga gaataacttc atcgccttca taaggacgat
     6241 tgacgaagcc gcggttattt ccggcatcca gtgtagccca gcgtgcccat attcctttcg
     6301 aatcggattc gatgagcgga tactttacac gcacgcggaa atcgccactg tccgtatcct
     6361 taacggcggt gatctttccc ggctgaagtc cgtgcatggc aggaagaagt cctgcggccg
     6421 atttttccgt tatgttgtca tggcgttcgt aaaaccattc gtcggaataa ccgaactgaa
     6481 tgtccgtgta ccaggtgctg tgtgcactga agctgtgcgt aacagatgaa atgaaggctg
     6541 tattattaaa gtgatcgccg agcccgtcga aggtgaatgt ttttccgggt ttggcgacat
     6601 tggtcccgta tatccgcacg cgacctttga tcttggtcat tttgctgcgg agaaactttg
     6661 catcggccca ggcagtgagt tcgccttctt ccagctttcc ggaatgctgc aggcggtagt
     6721 ttatgctgcc cattacatcg gaaaggccga gtgagttgag cgttccctct tccttgaaga
     6781 ccggagtgga aggaatgttg acgcccagtt cctgtttggt gaaatcccag tctttggagg
     6841 taacacctgc gtactgatcg cgtgcatcca tttccgcgtc gatctccacg atgtttgttc
     6901 cataagtgat ctcgagcgaa ggagaggtcg cttgtgcttt ttccggatct gcaacagtca
     6961 ctttgccgtc gtcaacgtaa acgagaagcc cgttcgcctc tgcgcgcgaa acaaggaagt
     7021 cccagtcggt gctgtaatac tgcaccatgt ctttatgcgt cactttcgtg gcagcaactt
     7081 ctttctgcac gccgtaggtg ccaagcagtt gttcaatgat ctcgctgtcc ttcttgtcga
     7141 agtaacatgc gttttttcgc tcgatcgtcg acttgatgat cttatccctt gcttccagaa
     7201 gcaacgtcga aggaccggta aggttcgctt tgatcgactg gcggatgatg atcccggaaa
     7261 agatctgctg cgtggaagtg tcttcctgcg tctggtaagt agcctcgata atgacctctt
     7321 ttcccggcag gtaatcgttg ccctcgctga gtttgaattc ctgctttgat gccgatccat
     7381 cgtaaagaac gatctccgcg acggaaatac ggttgatgcc cttgttcacc attacggacg
     7441 ccacaccagg attggaagaa actttttttc ctccgatcgt aaccgtatag acggggtgcg
     7501 agatcttttt gtgtcccggg atagtgtcct gtgtagaatt gatcttgggc atctcagaca
     7561 gttttaacgg gaggtaagat cagttgcgtg ccgggtacca ggcgacggta atttttcagg
     7621 tcattcgctt ctgcgatctg caggtaatac gattcgctgc cgtattcatt cctggcaata
     7681 ttgtagatgg tgtcgcccgc ggtgacgagg tggagtttgg tgatatcggg agattgccag
     7741 cttaaactgg ctctgtcgtt tccagcaaag ggtacctgta taaaggacgc tgaaagggtt
     7801 gcccttaagg gattt
//
LOCUS       DASTSF010000488         2041 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1873348,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000488 DASTSF010000000
VERSION     DASTSF010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2041
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1873348"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /gene="rplP"
                     /locus_tag="VFU15_14055"
     CDS             <1..207
                     /gene="rplP"
                     /locus_tag="VFU15_14055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015334428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEU4718962.1"
                     /translation="TRKPAEVRMGKGKGAPEFWVAPVKPGTILFEADGVPVETAREAL
                     RLAAQKLPVSTKFIVRADYSPENA"
     gene            229..432
                     /gene="rpmC"
                     /locus_tag="VFU15_14060"
     CDS             229..432
                     /gene="rpmC"
                     /locus_tag="VFU15_14060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEU4718963.1"
                     /translation="MKQKEISQMSLKELRDRLAEEKGHLHKLNLNHSVSPIENPLKIR
                     TTRRTIARIETELRKRELAETQN"
     gene            459..725
                     /gene="rpsQ"
                     /locus_tag="VFU15_14065"
     CDS             459..725
                     /gene="rpsQ"
                     /locus_tag="VFU15_14065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEU4718964.1"
                     /translation="MENTIKRKDRKEKTGIVISNKMAKSIVVAVERKVKHPKYGKFVK
                     KTSTFMAHDEKNECNVGDTVRIAETRPLSKNKCWRLIEITERAK"
     gene            754..1122
                     /gene="rplN"
                     /locus_tag="VFU15_14070"
     CDS             754..1122
                     /gene="rplN"
                     /locus_tag="VFU15_14070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002675043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEU4718965.1"
                     /translation="MIQQESRLSVADNSGAKEVLCIRVLGGTKKRYASIGDKIVVTVK
                     HALPSGNVKKGTVSKAVVVRTRKEISRPDGSYIRFDDNACVLLNAQDELRGTRIFGPV
                     ARELRDTNYTKIISLAPEVL"
     gene            1138..1461
                     /gene="rplX"
                     /locus_tag="VFU15_14075"
     CDS             1138..1461
                     /gene="rplX"
                     /locus_tag="VFU15_14075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004583587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEU4718966.1"
                     /translation="MAKLKIKKGDSVVIISGEHKDRDTRYKVVAVNPKDNRVFLEGLT
                     VKKHTRPTTKHPNGGIVDVPASVHISNLMMVGPDGQPTRVGRRLNEETGKLERYSKKS
                     EEVIK"
     gene            1461..2027
                     /gene="rplE"
                     /locus_tag="VFU15_14080"
     CDS             1461..2027
                     /gene="rplE"
                     /locus_tag="VFU15_14080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEU4718967.1"
                     /translation="MATAAYTPRMKKVYQEQVVPALLKKFSYGTNMEVPCLEKICINQ
                     GIGDAVADKKLIEGAIKEMGLITGQRAISTISRKDISNFKLRKGVPIGVKVTLRGEKM
                     YEFLDRLISAALPRIRDFRGINEKGFDGRGNYTLGITEQIIFPEIDIDKVNKILGMDI
                     TFVTTANTDEEALALLQEFGLPFKNNKK"
BASE COUNT          627 a          475 c          514 g          425 t
ORIGIN      
        1 acccgcaagc ctgcagaggt tcgtatgggt aagggcaagg gagctcccga attctgggta
       61 gcaccggtta agcccggaac cattctcttt gaagccgatg gagttcccgt ggaaacagcg
      121 cgcgaagcgt tgcgtcttgc ggcccaaaag ctgccggtat cgaccaagtt tattgttcgt
      181 gccgattatt cacccgaaaa cgcttaattc caagataaga acagagcgat gaaacagaag
      241 gaaatttcac agatgtcact gaaagaattg cgcgatcgtc tcgccgaaga aaaaggacat
      301 ttacacaagc tgaatcttaa ccacagcgtt tcgcctatcg agaatccgct gaagatccgc
      361 actacgcgcc ggacgatcgc caggatcgaa acggaactgc gtaagagaga acttgcagaa
      421 acccaaaact gatcacaaaa agatcaaacg gcagaaaaat ggaaaacacc ataaagcgga
      481 aagaccgcaa ggaaaaaacc gggatcgtta tcagcaataa aatggcaaaa tccattgttg
      541 tggctgttga acgtaaggta aagcacccca agtacgggaa gttcgtgaaa aaaacatcca
      601 ccttcatggc tcatgatgaa aagaatgagt gcaatgtggg agatacggtt cgtatcgctg
      661 aaacccgtcc gctgagcaaa aacaagtgct ggcgtctcat tgagataacg gaacgtgcaa
      721 aataatagta actgaatttg aaagtgttaa aagatgatcc agcaggaatc aaggctgtcc
      781 gtagccgata acagcggagc gaaagaagtt ctctgcatcc gtgtactcgg aggaaccaag
      841 aaacgctatg cttccatcgg cgacaaaatc gtcgttacgg taaagcatgc gttgccctcc
      901 ggaaacgtga aaaaaggaac ggtgtccaag gccgtagttg tcaggacacg caaggagatc
      961 agccgtcccg acggttctta tatccgcttt gacgacaatg cctgtgtgct tctcaacgcg
     1021 caggacgagc ttcgcggaac gcgtattttc ggacccgttg cacgtgagct gagagatacc
     1081 aattacacca agatcatttc actggcacct gaagtgcttt aaaaaggata cgataaaatg
     1141 gccaagctga aaattaaaaa aggcgattcc gttgtgatca tctccggaga acacaaggac
     1201 cgtgatacca ggtacaaagt ggttgccgtg aatccgaaag acaaccgcgt attcctggag
     1261 ggactcaccg tgaaaaaaca tacccgtccc acgacgaagc atcctaacgg tggcatcgtg
     1321 gatgtgccgg cgtctgttca tatttccaat cttatgatgg tgggcccgga cgggcagccg
     1381 acccgtgtcg gcagaaggct caatgaggaa accgggaaac tcgaacgtta ttcaaaaaaa
     1441 tctgaggagg ttattaaata atggctaccg ctgcatacac acccaggatg aagaaggtgt
     1501 accaggaaca ggtcgttccg gcgcttctca aaaaattcag ctatggaacg aacatggaag
     1561 ttccatgcct ggaaaagatc tgcatcaacc agggtattgg tgatgccgtt gccgacaaga
     1621 agctgatcga aggcgcaatc aaggaaatgg ggcttattac cggccagaga gctatttcga
     1681 ccatttccag gaaggatatt tcaaacttca agcttcgtaa aggcgtaccg atcggcgtta
     1741 aagtaacgct gcgcggcgaa aaaatgtacg agttcctcga tcgccttatt tctgctgctt
     1801 tgccgcgtat ccgcgatttt cgcggtatca acgaaaaagg ctttgacggg cgcgggaatt
     1861 acaccctcgg cattacggaa cagatcattt ttccggagat cgacattgat aaagtcaaca
     1921 agatcctcgg aatggatatc acattcgtga ccacggcaaa taccgatgaa gaagcgcttg
     1981 ctcttcttca ggagtttggc ctgccgttca aaaacaataa aaaataacta tctttgcagc
     2041 c
//
LOCUS       DASTSF010000489         4389 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1428223,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000489 DASTSF010000000
VERSION     DASTSF010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4389
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1428223"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1165)
                     /gene="rodA"
                     /locus_tag="VFU15_14085"
     CDS             complement(<1..1165)
                     /gene="rodA"
                     /locus_tag="VFU15_14085"
                     /inference="COORDINATES: protein motif:HMM:NF037961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HEU4718968.1"
                     /translation="MREKSESIFYNVDWFTVAIYAILVTIGWFNIYSAVYDEAKSNIF
                     DFSRIESKQLVFIGVSVIIAVSILIIDASFFTATAYILYAGVLLLNLAVVVLGTDIRG
                     SKSWFKIGGFGFQPAEFAKWATLLALAKFISGVDRVNFRRTFYMASLIICLPIAIIAV
                     LQNETGVALVFFGFIFVLYREGIFPGWLLMAGIASAASAVIGIKYGAIVIPDVIPPKG
                     VSVHYTIDMKALLFWLGSLAAAGVIYLLAVRRTFLNMLIVIGFIAGLTGVYLAAPIVL
                     EKIPEHQSGRIKTWLSLPVSKEISDRFDYNTRQSMIAIGSGGVNGKGYLQGTQTKFRF
                     VPEQETDFIFCTVGEEWGFMGTAGVVLLYLVLILRLIILSERQRSDFTRIYGYG"
     gene            complement(1167..3047)
                     /locus_tag="VFU15_14090"
     CDS             complement(1167..3047)
                     /locus_tag="VFU15_14090"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEU4718969.1"
                     /translation="MSSGIRKFILMGFAVIIAMLYIVRLFYVQVVDDQYKLDARNQAF
                     LYVTDYPPRGAIFDRNGKPLVVNQVAYDLMVVPRDLKDCDTMGICRILNITKDDFEKR
                     LIHFCGTLKAAKGDYRSYIFESQMLPELNARIQERLYKFNGFYVQSRTVRQYPVPIAA
                     HLLGYIGEVDDQTCAKDPYYRSGDYIGKSGIEKAYEEYLRGKKGVQIKMRDVHNNIKG
                     SFKDGKYDTAAVRGTDLTATLDENLQLYGEQLMQNKIGGLAAIDPSTGEILALVTSPT
                     YDPNLFVGRDFPKNYGILQNDTIGKPLYNRALQAMYPPGSTFKLMNSLIGQDEGVLTP
                     ETRYPCAGGYPPLGGHPKCEIHASPLDLTGAIQHSCNSYFTYVIKSIIDNPKYHGNTV
                     AGYNAWREYVLSFGVGKKIGTDLPFELKGNVPTAGYYDKVFGKNGWHAQTLAGAGLGI
                     GQAELLITPLQMANIICSIANRGYYYTPHIIRNIGNDPPLAKWSLKNYTLVRDTNYYN
                     VVINGMAEVVKAGTAAASQIPGVEMCGKTGTAQNPHGKDHSVFVCFAPRDHPKIAVAV
                     VVENAGWGAQWAAPIASLIVEKYLKGNISRPDVEQRMLNGDLLHQQPVPGAKPVPVHV
                     AN"
     gene            complement(3044..3562)
                     /gene="mreD"
                     /locus_tag="VFU15_14095"
     CDS             complement(3044..3562)
                     /gene="mreD"
                     /locus_tag="VFU15_14095"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEU4718970.1"
                     /translation="MVNDVVRNVIRFLLLVFVQGLILKNVEPGPYINPFLYVLFIMQL
                     PFETPPWVVLVASFFTGFCVDLFYGTLGMHMAACTLVGFFRPRLLRFMSPRDGYEFGA
                     QPTIQDMGRAWFITYAVIIIFVHHFVLFYLEIFSLHEFFNTLLRVIVSGAATLVLVVV
                     TQFLFYRSRETT"
     gene            complement(3552..>4389)
                     /gene="mreC"
                     /locus_tag="VFU15_14100"
     CDS             complement(3552..>4389)
                     /gene="mreC"
                     /locus_tag="VFU15_14100"
                     /inference="COORDINATES: protein motif:HMM:NF010532.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEU4718971.1"
                     /translation="MRNLAVFLVRHYLILLFIFLESISVYFLVQHNDFQHASAVSSAN
                     AFTGTLYEWRSGLTDYIGLREQNRSLSDRLAGLLSKQRSSFLFYGDSGQAFSDSAYHQ
                     RYVYLAAEIIDNTVDQRNNYMILDRGSKQGVTVDMGVVCAEGVVGYVREVSDNFCLVM
                     SVLHKDTRINASVKTDATFGMVAWDGTDYREATITDIPPHSKIKVGDTLVTTGFGDAF
                     PAGVNVGVVKKMGILSGEKTYTAQLLLCTDFKKVRHVFIVKDLVRDEVDGLKQKEGPN
                     GK"
BASE COUNT         1010 a         1231 c         1195 g          953 t
ORIGIN      
        1 cgccgtagcc gtaaatacgc gtgaagtccg atcgctgtct ctccgaaaga ataatgaggc
       61 gcaggatcag caccaggtaa agcagcacaa cgcccgccgt gcccataaat ccccattctt
      121 cgccaacggt gcagaaaata aaatccgttt cctgctccgg cacaaaacgg aatttcgtct
      181 gcgttccctg caggtaacct tttccattca ccccgcctga gccgatggcg atcatcgact
      241 ggcgcgtgtt gtaatcgaac cggtcggaaa tttcttttga aaccggaagg ctcagccagg
      301 ttttgatcct tcccgactgg tgttccggaa ttttttccag cacgatcggt gcggcaagat
      361 aaacacctgt cagcccggcg atgaacccga tcacgatgag catattcagg aatgtgcgcc
      421 ggacagcaag caggtaaata acgcctgccg ccgcaagcga gccgagccag aacaaaagcg
      481 ccttcatgtc tatcgtataa tgcacggaaa ctcctttcgg cggtatcaca tccgggatca
      541 cgatggcgcc gtatttgata ccgatcacag ccgaagcggc cgaagcgatc cctgccatca
      601 gcaaccaccc gggaaagatg ccttcacggt acagcacgaa aatgaatccg aaaaaaacga
      661 gtgcgacgcc ggtctcattc tgcagtacgg caatgatggc gatcggaagg cagatgatca
      721 gcgaggccat gtagaacgtt cgcctgaagt tgacccggtc gacaccgctg atgaatttcg
      781 cgagcgccag cagcgtcgcc cacttcgcaa actccgccgg ctggaaaccg aagccgccga
      841 tcttgaacca cgatttgctt ccgcgtatat cggttccgag aacaacgacg gcaagattca
      901 gcagcaggac gcctgcataa agtatgtaag ccgtggccgt aaaaaacgat gcgtcgatga
      961 tcaggatgct gacggcgatg atgacggaaa cgccgatgaa aacaagctgc ttcgattcga
     1021 tccggctgaa atcgaaaatg ttgctcttcg cttcgtcgta gacggccgaa taaatgttga
     1081 accagccgat cgtgacgaga atggcgtaga tcgcaacagt gaaccagtcg acattataga
     1141 atatgctttc gcttttttcg cgcatctcag ttcgctacgt gaacaggaac gggtttcgca
     1201 cccggaaccg gttgctggtg cagcaggtcg ccgttcagca tccgctgctc gacatcggga
     1261 cgtgatatgt ttcctttcag gtatttttcg acgatgaggc tggcgatcgg cgcggcccat
     1321 tgcgcgcccc agccagcatt ctccaccacc acggccaccg cgatcttcgg gtgatcacgc
     1381 ggagcgaaac agacgaacac ggagtgatcc ttcccgtgcg ggttctgcgc tgttcccgtt
     1441 tttccgcaca tctccacacc ggggatctgc gaagcagccg ccgttccggc cttcaccact
     1501 tccgccatac cgttgatcac gacgttgtaa tagttcgtgt cgcgcaccag cgtgtaattt
     1561 ttgaggctcc atttcgccag cggcggatcg ttgccgatgt tgcggatgat gtgcggcgta
     1621 taatagtaac cgcggttagc gatcgagcag atgatgttcg ccatctgcag gggcgtgatg
     1681 agcagctccg cctgtccgat gccgagtcct gcaccggcga gcgtctgcgc atgccatccg
     1741 ttcttgccga aaaccttatc gtaatacccg gcggtcggta cgttcccttt gagctcgaac
     1801 ggaagatccg tgccgatctt tttcccgacg ccgaagctga gcacgtactc gcgccaggca
     1861 ttgtagcccg ccaccgtatt gccgtggtat ttcggattgt cgataatgga tttgatgacg
     1921 taagtgaaat acgaattgca ggaatgctgg atggccccgg taagatcgag cggcgatgcg
     1981 tggatctcgc atttgggatg gccgccgagc ggcggataac cgcctgcaca gggatagcgc
     2041 gtttcaggtg tgagcacgcc ctcgtcctgc ccgatcagcg aattcatcag cttgaacgtg
     2101 gaaccgggcg ggtacatcgc ctgcagcgca cggttataca acggtttccc gatcgtgtcg
     2161 ttttgcagga tgccgtagtt cttcgggaaa tcacgcccga caaaaagatt cggatcgtac
     2221 gtgggactcg tgacaagcgc aaggatctct cccgtcgagg gatcgatcgc ggcgagcccg
     2281 ccgatcttgt tctgcatgag ctgttctccg taaagctgca ggttctcatc gagcgtggcg
     2341 gtcagatccg ttccgcgcac agccgccgta tcgtattttc cgtctttaaa gctccccttg
     2401 atgttgttgt gcacgtcgcg catcttgatc tgcacgcctt ttttcccgcg caaatattct
     2461 tcgtacgctt tttcgatccc gctcttcccg atgtaatcgc ccgaacggta atacggatcc
     2521 ttcgcgcatg tctggtcgtc cacctcgccg atgtagccga gcaggtgtgc cgcgattgga
     2581 acgggatact ggcgcaccgt tctcgactgc acatagaacc cgttgaactt gtagaggcgc
     2641 tcctgtatcc gtgcattcag ttccgggagc atctggcttt cgaagatgta gctgcggtaa
     2701 tcgcctttcg ctgctttcag ggtgccgcag aaatggatca gccgtttttc gaaatcgtct
     2761 ttcgtgatgt tcaggatacg gcagatgccc atggtgtcgc aatctttcag gtcgcgcgga
     2821 acaaccatca ggtcgtatgc cacctggttg acaacaagcg gcttcccgtt tcgatcgaaa
     2881 atggcgccgc gcggaggata gtcagtaacg tacaggaatg cctggttgcg tgcatcgagc
     2941 ttgtactggt cgtcgacaac ctggacatag aacagccgca cgatgtaaag catcgcgatg
     3001 atcaccgcga atcccatcag gataaatttc cgaatacctg aactcatgtt gtttctcttg
     3061 aacggtaaaa caggaactgg gttacaacca cgagcacaag cgtggctgca ccgctgacga
     3121 tcacccggag gagcgtattg aaaaattcat gcaggctgaa tatttcgagg tagaacagca
     3181 cgaagtgatg aacgaaaatg atgatgaccg cataggtgat gaaccatgcg cgtcccatgt
     3241 cctgtatcgt cggctgggcg ccgaattcat aaccgtcgcg gggcgacatg aaacgaagaa
     3301 gccgcggacg gaaaaaaccg accagcgtgc aggccgccat gtgcatgccc agcgttccat
     3361 aaaaaagatc cacgcagaag ccggtgaaaa aagaagcgac aaggaccacc cagggcggtg
     3421 tctcgaacgg aagctgcatg atgaaaagga catagaggaa cgggttgatg tacggacccg
     3481 gctcgacatt tttcaggatc aggccctgca cgaaaacgag cagcaggaag cggatcacat
     3541 tccggacgac gtcatttacc attgggcccc tccttctgtt tcaagccgtc cacttcgtcg
     3601 cgcacgagat ctttcacgat gaaaacatgc ctcacttttt tgaaatccgt gcaaagcagg
     3661 agctgcgccg tgtatgtttt ttcaccggaa agaatgccca tctttttcac cacaccgacg
     3721 ttgacgccgg caggaaacgc atccccgaac ccggtggtga cgagcgtgtc gcccactttt
     3781 attttcgaat gcggcggaat gtccgtgatc gtcgcttcgc ggtaatctgt tccgtcccac
     3841 gccaccatcc cgaacgtcgc gtccgttttc acgctggcgt tgatgcgcgt gtccttgtgg
     3901 agcaccgaca tcacgaggca gaaattatcg gaaacctcgc gcacatagcc caccacgcct
     3961 tccgcgcaca ccacgcccat atccacggta acgccctgct tcgatccgcg gtcgaggatc
     4021 atgtagttgt tccgctggtc gaccgtgttg tcgatgatct ccgccgcgag ataaacataa
     4081 cgctggtgat acgcgctgtc gctgaatgcc tgcccggaat cgccgtaaaa aagaaaggag
     4141 ctccgctgct tcgacagcag gccggccagc cggtcggaaa gactccggtt ctgttcgcgc
     4201 agcccgatgt aatccgtcag tcccgatctc cattcgtaaa gcgttccggt aaatgcgttc
     4261 gcggatgaaa cagcgctggc gtgctggaaa tcgttgtgct gaacgaggaa atagacagag
     4321 atgctttcca ggaaaatgaa caggaggatc aggtaatggc gtacgagaaa aacggcgaga
     4381 ttacgcatc
//
LOCUS       DASTSF010000490         3101 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_193904,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000490 DASTSF010000000
VERSION     DASTSF010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3101
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193904"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..917
                     /locus_tag="VFU15_14105"
     CDS             <1..917
                     /locus_tag="VFU15_14105"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEU4718972.1"
                     /translation="VGAAPLNLGPDTVLCSYAILQTGPGRVYSMWHDPFGNTESNNPY
                     YYATQPGLYWVTVTDSNGCVASDSVTFTAPLNRPPANLGNDTTVCTGHTVTLHTAASY
                     LHYTWQDGTHNSTYTAWLPGVYWVTVNDGCTISTDSIHIYWNDALPLDLGNDTMVCPG
                     GFPFTLSAPAGYDYLWQDGSSAANFTVNGPGTFWVNVVTTNGCMSRDTVDVSVCTGAD
                     ENAAAAFMIYPNPAGDFITVDPGSPGAQPVTADIFDPLGRNVFHAFYPAVSSALSIDV
                     SSLAEGVYIIRFSNSKTTYRVPMVISRH"
     gene            1033..>3101
                     /gene="paaZ"
                     /locus_tag="VFU15_14110"
     CDS             1033..>3101
                     /gene="paaZ"
                     /locus_tag="VFU15_14110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015695537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation bifunctional
                     protein PaaZ"
                     /protein_id="HEU4718973.1"
                     /translation="MNSTKDKVAVRMLDNYALGKWTAGEGNGQVLYNAITGDPVATAS
                     SKGLDFEAMLKYGRKTGGPLLRKMTFHERGRMLRALALHLLSKKEEFYKISWATGATR
                     SDSWVDIEGGIGNLFAYASLRRQFPDETFCVEGDVAKLSKGNTFIGHHICVPKEGVAI
                     HINAFNFPVWGMLEKVAVNWLAGVPAIVKPATLTSFLTEAVVREIVASGILPDGALQL
                     ICGSANGILDHVMNQDVVTFTGSASTGKMLKSHPRLLEESVHFNMEADSLNCCILGSD
                     VTPDKPEFDIFIKEVVREITTKAGQKCTAVRRIIVPENRVEEVRAALGKRLSQTVIGD
                     PNAEGVRMGSLAGNGQRKEVMEKVEQLSKTQKIIIGDFEKFEVKGADKNKGAFMPPVV
                     FLNENPFTNTDCHNIEAFGPVSTLMPYKSTEEAIELARLGKGSLVSSIITADDKIAKQ
                     YVLGAASMHGRILILNSDCAKESTGHGSPMPMLVHGGPGRAGGGEEMGGKRGVFHYLQ
                     RTAIQGSPTTISHVTNQYQYGGKYIEKGIHPFREYFEDLEIGETLITSTHTVSEDDII
                     NFANLSGDKFYAHMDPEALDGTIFKRTVAHGYFVLSRAAGLFVDPPKGPVLLNYGIDE
                     CRFTKPIYPGMTIGVRFTCKEKTENDKPLKAANGEEVKCGIVKWVVDVYDASPQDVRD
                     QYGLEGAT"
BASE COUNT          753 a          873 c          833 g          642 t
ORIGIN      
        1 ccgtcggtgc agcccctctc aatcttggtc cggacaccgt tctctgttct tatgcaattc
       61 tgcaaaccgg ccccggccgc gtgtattcga tgtggcacga tccgttcggt aatacagaat
      121 ccaacaaccc ctattattat gccactcagc ccggcctgta ttgggtgacc gtgacagaca
      181 gcaacggttg tgttgcttcc gattcggtca cgttcaccgc accgctcaac cgcccgcccg
      241 caaatctcgg caacgacacc accgtttgca ccggtcacac cgttacgctg cacaccgcgg
      301 cttcctatct gcattacacg tggcaggatg gaacacacaa cagcacatac accgcatggc
      361 tgccgggagt ttattgggtg accgtaaacg acggatgtac gatcagcacc gacagtatcc
      421 acatttactg gaacgatgcg ctgccgctgg atctcggtaa cgacacgatg gtgtgcccgg
      481 gaggatttcc tttcacgctt tctgctccgg ccggttatga ttatctctgg caggacggaa
      541 gcagcgcagc gaatttcaca gtgaacggac cgggaacgtt ctgggtgaac gtggtcacaa
      601 caaatggctg catgtcgcgc gacacggtgg atgtatccgt ctgcacaggc gcagatgaaa
      661 acgcagcggc ggcattcatg atttatccca acccggccgg tgattttatc actgttgatc
      721 cgggatcacc cggtgcgcag ccggtcaccg cagatatttt cgacccgctg ggaagaaacg
      781 tgttccatgc attttatccc gccgtttctt ctgcgttatc catcgatgtt tcttcactgg
      841 ccgaaggcgt ttacattatc cgcttcagca acagcaaaac aacctatcgc gttccgatgg
      901 tgatcagccg acactgaacc gcgcatcgat cgtgcatgat gtgagcatgg ccgggatgac
      961 ctttcccatc ccgacttttg ctaacttcat accccgaaat atcattttca gaaacatctt
     1021 gcacacacga tcatgaattc cacgaaagac aaagttgctg tccggatgct ggacaattat
     1081 gcgctcggca aatggaccgc cggtgaaggc aacgggcagg ttttatacaa tgcgatcaca
     1141 ggtgatcccg ttgcaacggc ctcgagcaaa gggctcgact tcgaagcgat gctgaaatat
     1201 ggtcgcaaaa ccggcggacc gctgctccgc aaaatgacct tccacgaacg cggccgcatg
     1261 ctgcgtgcgc tggcgcttca cctgctgagc aaaaaagaag aattctataa aatttcctgg
     1321 gccacgggcg ccacaagaag cgacagctgg gtggacatcg aaggcggcat cgggaacctg
     1381 tttgcctacg cgtcgctgcg ccggcaattt ccggatgaaa cgttctgcgt ggaaggtgac
     1441 gtggcgaagc tttcgaaagg aaatacgttc atcggccatc acatctgcgt accgaaagaa
     1501 ggtgttgcca ttcacatcaa cgcgttcaat ttcccggtct ggggaatgct ggaaaaagta
     1561 gcggtgaact ggctcgccgg tgttcccgct attgtgaaac ctgcgacgct gacctcgttc
     1621 ctcacggaag ccgtcgtaag agagatcgtc gcttcgggga tccttccgga cggtgcgctg
     1681 cagctgatct gcggctccgc caacggcatt ctcgatcacg tgatgaacca ggatgtggtg
     1741 acgttcaccg gctccgcctc taccgggaaa atgctcaaat cgcatccgcg ccttctcgaa
     1801 gaatccgtcc atttcaacat ggaagccgat tccctcaact gctgcatcct tggcagtgac
     1861 gttacgccgg acaaacctga atttgacatt ttcatcaagg aagtcgttcg cgagatcacc
     1921 acgaaagccg ggcagaaatg tacagccgtg cgacgcatca tcgttcccga aaaccgcgtg
     1981 gaggaagtgc gcgccgcact gggcaagcgt ctttcacaaa ccgttatcgg cgacccgaac
     2041 gcggaaggcg tacgaatggg ttcgctggca ggaaacgggc agcgcaaaga agtgatggag
     2101 aaagtggagc agctttcgaa aacgcagaag atcatcatcg gtgattttga gaaattcgaa
     2161 gtgaaaggcg ccgacaagaa taaaggcgcg ttcatgcccc cggtcgtttt cctcaatgaa
     2221 aatcctttca cgaacaccga ctgccataac atcgaagcgt tcggaccggt gagcacgctc
     2281 atgccgtaca agtcgacgga agaagcgatc gagctggcgc ggctcggcaa gggttcgctc
     2341 gtcagttcca ttatcacggc cgacgataaa atagcaaagc aatatgttct cggtgcggca
     2401 agcatgcacg ggcgcattct cattctcaac agcgactgcg caaaagaaag cacgggacac
     2461 ggttcaccga tgcccatgct cgtgcacggc ggacccgggc gcgcaggcgg aggcgaagaa
     2521 atgggcggaa aacgcggcgt gttccattac ctgcagcgta ccgcgataca gggttctccc
     2581 acgacgatct cgcacgtgac gaatcaatac cagtacggcg gaaaatacat tgaaaaagga
     2641 attcatcctt tccgcgaata ctttgaagac ctcgagatcg gtgaaacgct catcacttcc
     2701 acgcacaccg tttccgagga cgacatcatc aattttgcga acctcagcgg cgataaattc
     2761 tacgcgcaca tggatccgga agcgctcgac ggaacgatct tcaaacggac cgttgcgcac
     2821 ggttatttcg tgctttcccg cgcagccggg ttatttgtcg atccgccgaa aggccccgtg
     2881 ctgctgaatt acggcatcga tgaatgccgc ttcacgaaac ccatttatcc cggcatgacg
     2941 atcggcgtgc gttttacctg caaagagaaa actgagaacg acaaaccgct caaagccgcg
     3001 aacggagaag aagtgaaatg cggcatcgtg aaatgggtgg tcgatgtgta cgacgcaagt
     3061 ccgcaggatg tgcgcgacca gtacgggctt gaaggcgcca c
//
LOCUS       DASTSF010000491         3640 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_916379,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000491 DASTSF010000000
VERSION     DASTSF010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3640
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_916379"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(16..1056)
                     /locus_tag="VFU15_14115"
     CDS             complement(16..1056)
                     /locus_tag="VFU15_14115"
                     /inference="COORDINATES: protein motif:HMM:NF028129.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5074 domain-containing protein"
                     /protein_id="HEU4718974.1"
                     /translation="MKKTVLVASAAILLFGCKKDEPAPPSGSNSASTSIVAGEGALVA
                     CEGNFQGGNAKVSYYKFSDGSVVTDLFSTANNRPLGDVCQSISFSNGLAYIVVNNSGK
                     IEEVNPSSYVSQRIVNGFTSPRYMLQVAPGRAYVTDLYANGVWIMNTVNNTISGMISV
                     PGETEAMEPVNGEIFVTSPDRDQLYVINSVTNTITDSITVAKGGNSMVSDANGKLWVL
                     CYGDYFTSAPGGLYRIDPQTHTVEASWPFTTAESPTRLCCNSTRDTLYYIDNDIYRMP
                     VTNAALPSTPFISAGTHSFYGLAVRPSDNELFVTDAVDYVQQGHLLRYTRQGVLTDDK
                     QVGIIPGSIYFY"
     gene            complement(1083..3047)
                     /locus_tag="VFU15_14120"
     CDS             complement(1083..3047)
                     /locus_tag="VFU15_14120"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU4718975.1"
                     /translation="MNRKAGSAVLFFTCLCACVPLVATGQVTKDTANAKTTDADMQYG
                     LKGVIIISTPGSSGPGGLVVSRPDSGITEQQQCGTLNDLLGSSGAMFSKYYGANGLSS
                     PGLRGSSPQQTPVVWNGLNLQAVTHGQVDLSLFPSILFDNVSVQPSGGSSLNGDAPGG
                     GITGVSYTPYMSRQFRFSALAGSFGENSLALSFGDGKPKYAYDIKAFRQQAVNNFPFD
                     NPYVPGNELTKLQHAGFFQQGAAGDFNYEFRNRRNLSVHSWVQETERDLPPTLLTNPN
                     ASWQHDYALRTILTFSDFSHDKWWYSASAAVVHNGLVFYDGYTQPASNTDEWEYVSDI
                     HFRKHFGEGYFSAGLCNQYSVAVVTQFIPLHHQERPAAYLSFQRQFHKLQLSGSAREE
                     MYDAKWENPVIRFGARYDIRNWIAVYGNVANTYRVPTFNELYWSPGGNPNLKPESSFG
                     QELTVEFSRGGSYINGFYSATVYNRNVRNWILWVPGPAYWSPENVLAVRSRGIEHRLQ
                     ATCKLNSFTFTLNGYYEFNRTTKLKTTIRGDSSLGLQMIYVPQQRISGMLRIAWKYWY
                     VQYVHEFVDIRFTAADHSEYLPAFNTGQFTCGTSFVHRNEKAELFIRINNAWNTFYMV
                     TADRPMPGRSYEVGVTFRFEKKRDVFPAVK"
     regulatory      complement(3145..3348)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(3379..>3640)
                     /locus_tag="VFU15_14125"
     CDS             complement(3379..>3640)
                     /locus_tag="VFU15_14125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718976.1"
                     /translation="LYTPIGLLYEGLWYLFVYEIYRINYNVSNNEMFFENSERLPVCE
                     FRWSAAGIFKQLPDLAAARELMPQMHLWSGNFPGWKLKTCGF"
BASE COUNT          866 a          959 c          937 g          878 t
ORIGIN      
        1 ataactgtta cccgttcaat agaaatagat cgagccggga attattccca cctgcttgtc
       61 atcggtcagc acgccttgtc ttgtgtagcg aagcaggtgt ccctgctgga cgtaatcaac
      121 ggcatcggtg acgaatagtt cattgtcgga cgggcgcacg gcaagcccgt aaaagctgtg
      181 cgtgcccgcg gatatgaacg gtgtcgacgg cagcgctgcg ttcgtaacgg gcatgcggta
      241 aatgtcgttg tcgatgtaat agagcgtgtc gcgcgtggaa ttgcagcaca ggcgcgtggg
      301 actttccgcc gtggtgaacg gccaggaagc ttccaccgtg tgcgtctgcg gatcgatgcg
      361 gtaaagtccg ccgggtgcgc tggtaaaata atctccgtag caaagcaccc agagctttcc
      421 gttcgcatcg ctcaccatgc tgttgccgcc cttcgcgacc gtgatgctgt cggtaattgt
      481 attcgtcacg gaattgatca cgtacagttg atcacggtcc ggactcgtca caaaaatttc
      541 tccgttcacc ggttccatcg cttctgtctc gccgggaact gaaatcatcc cggaaatggt
      601 attgttcacg gtattcatga tccacacgcc gttcgcgtaa aggtccgtga catacgcacg
      661 cccgggcgcc acctgcagca tgtaacgcgg agaggtgaaa ccattcacga ttcgctggct
      721 tacataagag gaaggattca cttcttctat tttcccggaa ttatttacaa caatgtaggc
      781 gagcccgttc gaaaaactga tgctctggca aacatcgccc agcggacgat tattcgccgt
      841 cgagaaaaga tcggtgacta cggagccgtc gctgaattta taatagctca ctttcgcatt
      901 cccgccctgg aaattccctt cgcaggcaac gagcgcgcct tccccggcaa caatggatgt
      961 cgatgcgctg ttcgaaccgg aaggcggcgc cggttcgtct tttttgcaac cgaagaggag
     1021 aatagcggcg gatgcaacga gtactgtttt cttcattttt ttcttttttt agtagatatt
     1081 aattacttaa ccgccggaaa aacgtcgcgt tttttttcaa agcgaaacgt aactccgact
     1141 tcataagatc ttcccggcat ggggcggtca gccgtgacca tgtaaaatgt attccatgca
     1201 ttatttatgc gaataaacag ttcagctttt tcatttctgt gaacgaacga agttccgcag
     1261 gtgaattgcc cggtattgaa cgccggcagg tattcggaat gatcggctgc cgtaaaacgg
     1321 atgtccacga attcgtgcac gtactgaacg taccagtatt tccacgcgat gcgaagcatc
     1381 ccgctgatgc gctgctgggg aacatagatc atctggagcc cgagtgagct gtcgccccgg
     1441 atcgttgttt tcagttttgt tgtacggtta aattcataat agccgttcaa cgtaaaagtg
     1501 aatgaattca atttacaggt tgcctgcagg cgatgttcga tgcccctgct ccttactgcg
     1561 agcacgtttt ccggcgacca gtaagcaggc ccggggaccc acaggatcca gttccggacg
     1621 ttgcgattgt aaaccgttgc agaataaaaa ccgttgatat agcttcctcc ccgggagaat
     1681 tccacggtca gctcctgccc gaagctgctt tccggcttca ggttgggatt tccgcccggc
     1741 gaccagtaca gttcattgaa ggtcggaacg cggtacgtgt tggcgacatt tccataaaca
     1801 gctatccagt tccggatatc ataccgcgca ccgaaacgaa taaccgggtt ctcccacttt
     1861 gcgtcataca tttcttctct tgcgcttccg gacagctgca gcttatggaa ctgccgctgg
     1921 aagctgaggt aagccgccgg ccgctcctgg tgatgaagcg gaatgaattg agtcacgaca
     1981 gccacgctat actggttgca gagcccggca gaaaagtagc cctctccgaa atgtttccgg
     2041 aaatgaatat ccgacacgta ttcccattca tccgtattgg aagcaggctg tgtatacccg
     2101 tcgtaaaaca caagcccgtt gtgaacgaca gcagcggatg cggaatacca ccatttatcg
     2161 tgactgaaat cggagaacgt taaaattgta cgcaaggcat aatcgtgctg ccacgatgcg
     2221 ttcgggttgg tgagcaatgt cggcggaaga tcacgctccg tttcctgcac ccaggagtga
     2281 acggaaagat tgcgcctgtt gcggaattca tagttaaaat ccccggctgc tccctgctgg
     2341 aaaaaaccgg catgctgaag tttcgttagt tcatttcccg gcacatacgg attatcaaac
     2401 gggaaattgt tcaccgcctg ctggcggaaa gcctttatgt cgtaagcata cttcggtttc
     2461 ccgtcgccga aactcaatgc gagcgaattc tccccgaaac ttccggccag cgcagaaaac
     2521 cggaactgcc tgctcatgta cggtgtgtag ctcacgcccg ttattcctcc gcccggcgca
     2581 tcgccgttca gtgaagatcc gcccgaaggc tgcacgctta cattatcaaa aaggatggac
     2641 gggaaaagcg aaagatctac ctgcccgtgc gtcacggcct gcaggttcag tccgttccag
     2701 acaacaggcg tttgctgcgg actgctgccg cgaagcccgg gagaggaaag tccgttggct
     2761 ccgtaatatt ttgagaacat ggccccggag cttcccagca ggtcgttcag tgttccgcat
     2821 tgctgctgtt cggtaattcc ggaatcgggc cgggaaacga caagtccgcc gggaccggaa
     2881 gagcccggcg ttgaaatgat gatgacgccc ttcagcccgt attgcatgtc tgcatccgtg
     2941 gtttttgcgt tcgcggtgtc cttcgtcacc tgcccggtcg caaccagcgg aacgcacgca
     3001 caaaggcacg taaaaaataa aacggcggaa cctgctttcc tgttcatggt gcacgatctt
     3061 ttcccggatc attgaacctg ccgcagcgca gaaaaaaaag aaggagagaa atgactggca
     3121 cttcctctcg ccttcttccc gaggctacgg atgattcaca gcggcaggtc ttctgactcg
     3181 ttccgctcac cggcgccttc cctttctcaa tatgaaaagt ggcttgcaga ttccggtgag
     3241 ttttcaatgg aactcacagc agcgggtact gttcccggtt ctcacgggat tcccttttaa
     3301 ttcagtgcaa agttgaaatg aactgaaacc gttgcggggc aaatgtagaa aaaagtcgca
     3361 acacggctca gcggtagctc aaaatccaca ggtctttagc ttccaccctg gaaaatttcc
     3421 tgaccacaga tgcatttgcg gcatgagttc ccgggctgcc gccagatccg gaagttgctt
     3481 gaatattccg gcagcagacc accgaaattc acaaacaggc aagcgctcag aattttcaaa
     3541 aaacatttca ttatttgata cgttgtaatt tattcggtat atttcataga caaacaggta
     3601 ccataaacct tcgtaaagta ggccgattgg ggtgtataag
//
LOCUS       DASTSF010000492         5925 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_91085,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000492 DASTSF010000000
VERSION     DASTSF010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5925)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5925)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5925
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91085"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            115..381
                     /locus_tag="VFU15_14130"
     CDS             115..381
                     /locus_tag="VFU15_14130"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEU4718977.1"
                     /translation="MAEIFKSMDHDSRVKILKLLCSAKNKRMTVKNICETMEMEQSIV
                     SRHLGILKRSGVLERKNEGGKVFFGLNYNTCTGSMLEKWLGACK"
     gene            406..1824
                     /gene="atpD"
                     /locus_tag="VFU15_14135"
     CDS             406..1824
                     /gene="atpD"
                     /locus_tag="VFU15_14135"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016193351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEU4718978.1"
                     /translation="MKKELLQNGERMATGEIVSVRGSVVDSVFAEALPSIHTLLYAGD
                     KDEIAIEVLAQLGNNHVRGIALTPTQGLARGMKVKSTGAPLEVNVGKETLGRMFNVFG
                     KTIDHKAALQGLEQRSVHQLPPALMNRSAKSEVFETGIKAIDVLVPLERGGKAGLFGG
                     AGVGKTVLLTEMIHNMVGHHKGVSMFCGIGERCREGHELYHDMKEAGVLENMVMMFGQ
                     MNEPPGARFRVGHAALTMAEYFRDDEHRDVLLLIDNIFRFIQAGMEVSGLMGQMPSRL
                     GYQPTLGTELSKLEERIANTETAAITSIQAVYVPADDLTDPAAVHTFSHLSASIVLSR
                     KRAGEGLYPAIDLLQSGSKMATPAIIGERHYNIAQQIRETMAGYEEIKDIIAMLGLEQ
                     LSAEDRKLVNRARRIERFLTQPFFTTEQFSGIKGKDVSLEDALSGCERILSDEFSGFP
                     ESAFYMTGTISDVIGKAKKVKP"
     gene            1838..2230
                     /locus_tag="VFU15_14140"
     CDS             1838..2230
                     /locus_tag="VFU15_14140"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011022411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEU4718979.1"
                     /translation="MLSLKILLPYRVFATLENVQRITAETYEGSFGILPRRLDCVAAL
                     VPGILSYGTENGEMKYVAVDEGILIKTGPEVIISVRNAIGGTDLGKLRESVEKEFLNL
                     DENEKNLRSTLAKLESGFIRTMEKFRRE"
     gene            2233..2556
                     /locus_tag="VFU15_14145"
     CDS             2233..2556
                     /locus_tag="VFU15_14145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011022410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HEU4718980.1"
                     /translation="MKTEPENRQQQLSREIGGKEMRKLKAQRQPRRSAWAGFGVFGIV
                     GWSVVVPTLAGAMLGRWVDQKYPQPFSWTLTLMITGLAAGCVMAWRWIKKEHTDMHQK
                     TEDKK"
     gene            2553..2861
                     /locus_tag="VFU15_14150"
     CDS             2553..2861
                     /locus_tag="VFU15_14150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014150149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HEU4718981.1"
                     /translation="MTVLFSLTIVFVAGIILGVIFFGGLWLTVTKGMRTALPGLVFLL
                     SFFLRTGIVLPGFYFLAAGDWKKMLAGIAGFILARFLVMSVTRNAKLKTAQQNEGGVA
                     "
     gene            2858..3559
                     /locus_tag="VFU15_14155"
     CDS             2858..3559
                     /locus_tag="VFU15_14155"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEU4718982.1"
                     /translation="MKLSPDQSVLWQHGFVVINSTLVWTWIIMIVMATMSWLVTRRLS
                     TGIRISRWQSVLEILVTGISKQIKDVGLKQPEKYIGFLGTLFLFIAVSNLCIIFPFYV
                     PPTSSLSTTAALAISVFIAVPFFGILEGGLKGYLRSYLQPSFIMLPFNIISELSRTLA
                     LAVRLFGNIMSGGVIVSILLSITPFVFPVVMNALGLLTGMVQAYIFSILATVYIAAAT
                     SESRITKQISTTRKN"
     gene            3565..3834
                     /locus_tag="VFU15_14160"
     CDS             3565..3834
                     /locus_tag="VFU15_14160"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020606061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEU4718983.1"
                     /translation="MDSTTIIAIVSIVAAGLTTAIGCMIPALGQGKAIATALSSIAQQ
                     PDAEQTITRTLFVGLAMLESLAIYCFVISMIIIFANPFWNHFIAK"
     gene            3843..4619
                     /locus_tag="VFU15_14165"
     CDS             3843..4619
                     /locus_tag="VFU15_14165"
                     /inference="COORDINATES: protein motif:HMM:TIGR03321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEU4718984.1"
                     /translation="MLINWFTVTAQVVNFLILVWLLKRYLYKPVLNAIDEREKKIAEQ
                     VGSAEKKEADAIKAQEEFRKKNETFDNERSAMMNKAVLEVKTEQQRLLEEARSGAEEM
                     RRKLDAALQEDQDRLAKEITRKTKEEVFALTGKLLKDLAGKELDEQIAAVFTERLADL
                     SGEEKKKMAAAMKDAQQPVVVRSGTALKEEQKKKLEEAIKKSFGDVALQFVTAAEMTA
                     GIELTAGGYKMAWSIPDYLSSAGKMIDEALINGIENKKSA"
     gene            4631..>5925
                     /locus_tag="VFU15_14170"
     CDS             4631..>5925
                     /locus_tag="VFU15_14170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015773160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternate F1F0 ATPase, F1 subunit alpha"
                     /protein_id="HEU4718985.1"
                     /translation="MTEGSLDRIVENAFAQIGHARENYVHSLLPRETGKVMSVSEGIV
                     KVAGLPGTGFDELLKFPNGIFGIAFNVDPEEIGVILLGDDSLLKAGDEVERTGRVMDV
                     PVGNSLIGRMVNPLGEPIDGQGPVSFSQRLPIEREAPAILDRAPVSVPLQTGIKVIDA
                     LVPVGRGQRELILGDRQTGKTSIAVDAIINQKDKDVICVYCAIGQRAASVAKVVYSLR
                     ENHAMKHTIVVATEGSDSPGLQYIAPYAATSIAEFFMEQGRDVLIVYDDLTHHARAYR
                     ELSLLLRRPPGREAFPGDIFYIHSRLLERATHLDKAHGGGSLTALPVIETEAQNISAY
                     IPTNLISITDGQLYLSPRLFELGMFPAVDVGKSVSRVGGKAQLQAFRSVAGDLKLAYA
                     QFEELETFARFGTKLDDSTQKIIAHGKRIRECLKQQEQQP"
BASE COUNT         1494 a         1467 c         1727 g         1237 t
ORIGIN      
        1 atttgcgttg atcattgtct ttccgggcgc aggcagatat gcatctattt gcatagttga
       61 ataaatgcaa tccagctgat gcaaacacgg aacgtccctc aaagtccgca cgaaatggcc
      121 gagatattca aatcgatgga tcatgattcg cgtgtgaaga ttttaaagct gctgtgcagc
      181 gcaaaaaaca aaagaatgac agtgaagaac atctgtgaaa caatggagat ggagcaatcg
      241 atcgtttcgc gtcacctcgg gatcttaaaa cgaagtggtg tgctcgaacg caagaatgaa
      301 ggtggaaaag tttttttcgg attgaattac aacacctgca cgggaagcat gctggaaaaa
      361 tggctgggcg cgtgcaaata aggaaaacgg tttgtaaaaa ataaaatgaa aaaggaactt
      421 ctgcagaacg gcgaaaggat ggctacaggc gaaattgtgt ctgtgcgggg aagcgttgtc
      481 gacagcgttt ttgcggaagc gcttccttcc attcacacgt tgctgtacgc cggggacaag
      541 gacgagatcg cgatcgaggt gctggcgcag ctcggaaata atcacgtgcg ggggattgca
      601 ctgacgccca cgcagggact tgcgcgcggc atgaaagtga aaagcaccgg cgctccgctg
      661 gaggtgaacg tcgggaaaga aacgctcggg cgcatgttca atgttttcgg aaaaacgatc
      721 gatcataaag ccgcgctgca gggactcgag caacgcagcg ttcaccagct cccgccggcg
      781 ctgatgaacc gttcggcaaa atcggaagtg tttgagaccg gtatcaaggc tatcgacgtt
      841 cttgttccgc tggagcgcgg aggaaaagcc gggctgttcg gcggagcggg cgtgggcaag
      901 accgttctgc ttacggagat gatccacaac atggtcgggc atcacaaagg tgtaagcatg
      961 ttctgcggga tcggcgagcg atgccgggaa ggacacgagc tgtaccacga tatgaaagaa
     1021 gcgggcgtgc tggaaaatat ggtgatgatg ttcggacaga tgaatgaacc gcccggtgcg
     1081 cgtttccgtg tgggccatgc ggcgctgaca atggcggaat atttccgcga cgacgaacac
     1141 cgcgatgtgc tcctgctcat tgataatatt ttccgcttca tccaggcagg catggaagtg
     1201 tcgggcctta tgggacaaat gccgtcgcgt ctcggttacc agcctacgct gggcacggag
     1261 ctgtcgaagc tcgaagaacg cattgcaaat acggaaacag cagcgatcac gtcgatacag
     1321 gccgtttatg ttcccgcgga tgatcttacg gatcccgcgg cggtgcatac gttttctcat
     1381 ctttctgctt ccatcgttct ttcacgcaag cgcgcaggcg aaggtcttta tcccgcgatc
     1441 gacctgctgc agtcgggttc gaaaatggcg acgccggcga tcatcggcga aagacattac
     1501 aacatcgcgc agcagatacg cgaaacaatg gccggttacg aagagatcaa ggacatcatc
     1561 gccatgctgg gcctcgagca attgtcggcc gaagacagga aacttgtcaa tcgcgccagg
     1621 cggatcgagc gttttctcac acaacctttt ttcaccacgg agcagttcag cgggatcaaa
     1681 ggcaaagacg tcagtctcga agatgcgctg agcggatgtg aacgtattct cagcgatgag
     1741 ttcagcgggt ttccggaaag cgctttttac atgaccggga cgatcagcga tgtgatcgga
     1801 aaagctaaaa aagtcaaacc atgaaacgtg cggaaccatg ctgtcgctga aaatacttct
     1861 gccttaccgg gtcttcgcca cgctggaaaa cgtgcagcgc attaccgcgg aaacgtacga
     1921 aggttcgttc gggatccttc cgcgcaggct ggattgtgtg gcggcgctcg tgccgggaat
     1981 tttatcgtac ggaacggaaa acggggaaat gaaatatgtc gcagtcgatg aaggcatact
     2041 gatcaaaaca gggccggaag tgatcatttc cgttcgtaat gcgatcggcg gaacggatct
     2101 cggcaagctg cgcgaatcgg tggaaaaaga atttctgaac ctggatgaga atgaaaaaaa
     2161 tctgcgctcg acgctcgcga agctcgaaag cgggttcatc cgcaccatgg aaaaattccg
     2221 gagggaataa aaatgaaaac agaaccggaa aacagacagc agcagctcag ccgcgagata
     2281 ggcgggaagg aaatgcgcaa gctgaaggca cagcggcagc cgcgtcgcag cgcgtgggcc
     2341 ggcttcggtg ttttcggcat cgtgggatgg tcggtcgttg ttcccacgct cgccggggcg
     2401 atgctgggaa gatgggtgga tcaaaaatac ccgcagccgt tctcgtggac gcttacgctc
     2461 atgatcaccg ggcttgccgc aggatgtgtc atggcctggc gctggataaa aaaagaacat
     2521 acggacatgc atcaaaaaac ggaggacaaa aaatgacagt acttttttca ttgacgatcg
     2581 tttttgtagc cggcattatt ctcggcgtga tctttttcgg aggtctctgg ctgacggtga
     2641 caaaaggaat gagaaccgcc ctgccgggcc ttgttttcct gctcagcttt ttcctgcgta
     2701 cggggatcgt tctccccggt ttttatttcc tcgcggcagg cgactggaaa aaaatgctgg
     2761 ccggcatcgc gggatttatc cttgcgcgtt tcctggttat gtccgttacg cggaacgcaa
     2821 agctgaaaac cgcgcagcag aatgaaggag gtgtcgcatg aagctcagcc cggatcagtc
     2881 ggttttgtgg cagcacggtt ttgtcgtgat caattccacc ctggtgtgga catggatcat
     2941 catgatcgtg atggcgacga tgtcgtggct ggtcacgcgc aggctcagca cgggcatacg
     3001 catctcgcgc tggcaaagcg ttcttgaaat tcttgtaacg ggcatcagca aacagataaa
     3061 agatgtcggg ctgaaacagc cggagaaata tatcggcttc ctcggaacgc tgtttctttt
     3121 cattgccgtg tccaacctct gcatcatttt cccgttctac gttccgccca ccagctcgct
     3181 ttcgaccacg gcggcgcttg ccatcagcgt tttcatcgcg gttccctttt tcgggatact
     3241 cgaaggcgga ctgaaaggtt acctgagatc ttacctgcag ccttcgttca ttatgcttcc
     3301 gttcaacatc atcagcgaat tgtcgagaac gctcgcgctc gctgtgcggt tattcggcaa
     3361 catcatgagc ggcggtgtga tcgtgagcat actgctgagc attacgccgt tcgttttccc
     3421 ggtcgtgatg aacgcgctcg ggttgcttac gggaatggtg caggcttata ttttcagcat
     3481 actcgcaaca gtgtacatcg ctgctgcgac aagcgaatca cgcattacaa aacaaatcag
     3541 cacaacacgt aaaaattgaa aaccatggat agcacaacaa taatcgcaat cgtttccatt
     3601 gtcgctgccg gacttacaac tgcgatcggc tgcatgatcc ccgcgctggg acagggaaag
     3661 gcgatcgcca ccgcgctgag ctcgatcgcg cagcagccgg atgccgagca gacgatcacc
     3721 cggacgttat tcgtcgggct ggcgatgctc gaatcgctgg ccatctattg cttcgtcatt
     3781 tcgatgatca tcattttcgc caacccgttc tggaatcatt tcatcgctaa ataaaccgcg
     3841 tcatgctgat caactggttt acggtaaccg cacaagtcgt caacttcctg attctcgtct
     3901 ggctgttgaa gcgttacctg tacaaacctg ttctgaacgc cattgacgaa cgcgaaaaga
     3961 aaattgcgga acaggtcggc agtgcggaga agaaagaggc agacgcgatc aaagcgcagg
     4021 aagaattccg gaagaagaac gaaacgttcg acaacgagcg cagcgctatg atgaataaag
     4081 ctgtactcga agtgaaaaca gaacaacagc gactgctgga agaggcaagg tccggtgcgg
     4141 aggaaatgag acggaaactg gatgccgccc tgcaggaaga ccaggatcgc ctggcaaaag
     4201 agatcacgcg caaaacgaag gaagaggtgt tcgcacttac cggtaaatta ctgaaagacc
     4261 tcgccggaaa agagcttgac gaacagatcg ccgctgtttt tacggaacgc ctggcggatc
     4321 tttccgggga agaaaagaaa aaaatggcgg cagcgatgaa agatgcacag caaccggttg
     4381 tcgttcgcag cgggacggcg ctgaaggaag agcagaagaa gaaactggaa gaggcgataa
     4441 aaaaatcatt cggcgatgtt gcattgcaat tcgtcacagc ggcggaaatg accgcaggga
     4501 tcgaattgac ggcaggcggg tacaagatgg cgtggagcat cccggattat ctttcctcgg
     4561 cgggaaaaat gatcgacgaa gcgctgatca acggaataga aaataaaaaa tcagcttagc
     4621 acgaagcaag atgacggaag gcagcctcga caggatcgtt gaaaatgcat ttgcacagat
     4681 cggccatgcg cgggagaact acgttcattc gcttctgccg cgcgaaaccg ggaaagtgat
     4741 gagtgtttcg gaaggcatcg tgaaagttgc aggactgccc ggaacaggtt tcgatgagct
     4801 gctgaaattt cccaatggaa ttttcggcat tgcgttcaat gtggatccgg aggagatcgg
     4861 cgtcatcctg cttggcgatg attccctgct gaaagcgggc gacgaagtgg aacgcaccgg
     4921 tcgtgtgatg gatgtgccgg tgggaaattc gctgatcggc cggatggtga atccgctcgg
     4981 cgaaccgata gacggacagg gtcctgtttc tttttcacaa cgcctgccca tcgaacggga
     5041 agcgcccgcg atcctggacc gcgcccctgt aagcgttccg ctgcagacgg gaatcaaagt
     5101 gatcgacgcg ctcgtcccgg tgggacgcgg gcagcgtgag ctgatcctcg gcgaccggca
     5161 aacggggaag acatcgatcg cggtggatgc gatcatcaac cagaaagaca aagatgtgat
     5221 ctgcgtgtat tgcgcgatcg ggcagcgcgc agcttccgtg gcgaaagttg tttacagtct
     5281 ccgcgaaaat catgcgatga agcataccat tgttgtggca acggaaggaa gcgattcgcc
     5341 ggggctgcaa tacatcgcac cgtatgccgc gacgagcatt gcggaatttt tcatggagca
     5401 gggccgcgat gtgctcatcg tgtacgacga tctcacgcat cacgcgcgcg cctaccgtga
     5461 gctttccctg ctgctccgcc gcccgccggg aagagaagca tttcccggtg atattttcta
     5521 cattcattcg cgcctgctcg agcgggcgac gcatcttgac aaagcgcatg gcggcggctc
     5581 gctcaccgcg cttcccgtga ttgaaaccga agcgcagaat atttccgcct acatccccac
     5641 gaatctcatt tccatcaccg acgggcagct ctatctttcc ccgcggctgt tcgagctggg
     5701 catgttcccg gcggtcgatg tgggcaaatc cgtttcacgc gtaggcggga aagcgcagct
     5761 gcaggcgttc cgttccgttg cgggagacct gaagctggcc tatgcacaat tcgaagagct
     5821 ggaaacgttc gcgcgtttcg gaacgaagct cgacgacagc acgcagaaaa taattgcgca
     5881 cgggaaaagg atacgcgaat gcctgaagca gcaggaacag cagcc
//
LOCUS       DASTSF010000493         6979 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1308865,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000493 DASTSF010000000
VERSION     DASTSF010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 6979)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6979)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6979
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1308865"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1890
                     /locus_tag="VFU15_14175"
     CDS             <1..1890
                     /locus_tag="VFU15_14175"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEU4718986.1"
                     /translation="AGGNMWLGCYGAGVLCISPDEKITVYDDSTGLPTNNVLTLFQDA
                     KGNTWVGTLQGGVVKIAPGGKFSYFTKKDGLGDNSVWSITADGLGNIFFGTNDNGISC
                     YDGKQFVNISAKEGISSDLVYALVADDKNRLWVGTDKGVNRVSFGKNFSIISIKYFGE
                     SDGLRGTEISQNALYMDASDNIWMGTNEGLIRYQPKYDYINDNPPRLELTAIRLFYQA
                     ADWAKMSFKVDPRTNLPIDPEFSWKDNHITFDFQALTTDNVKYQFMLEGLDADWSPPT
                     TTTSAVYTNIPPGKHYVFRVKAVNRDGFWSKDVLAYSFAVNPPFWQTWWFYTIIVVLA
                     AASIFAFIRWRTARLEKEKKVLEDRVQERTQELRVANDQLSLAYTDIKDSINYAQRIQ
                     QAILPLGGEISKVLPDHFIFFRPRDVVSGDFYWFYPKGDKVFFAAVDCTGHGVPGAFM
                     SIIGNSLLHEIMNETETPSPAEILNRLREKLIAALRQKGADAESKDGMDMVLCCYDRK
                     GSKLTFSGANNPLYLVSGGKLEEFKNDKQPIGVYGDEMKPFTQKEITVGKGDVIYIFS
                     DGYPDQFGGEKGKKFLYTRFKQMLVEIAPLAMSQQDQKLEENFRKWKGANDQVDDVLV
                     MGFRF"
     gene            complement(1894..3198)
                     /locus_tag="VFU15_14180"
     CDS             complement(1894..3198)
                     /locus_tag="VFU15_14180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718987.1"
                     /translation="MKKISTALSRFTVPLILLVSVWVTANVNWGAQHRKYVITSDGKG
                     YYAYLPAIFIYHDLNFSFFDTIESNYYDEHTKYDYRTGVNGKVTDKYFCGVAVLQAPF
                     FLAGHAMTLLSGDPADGYSKWYVIFTGIGALFYLGTGLVFLRKFLRAHGANEGQSAFI
                     LAVIYFGTNLFYYSIVEPCMSHVYSFAMVSLFLFYGKSWIDTANRKHAAMACLAFGLI
                     AILRPVNVLVAAWLLFEAKGFLPLLKKKAALLREPLMLFLCLLLIALPVFVQLMLYKL
                     STGSFFIDTYSEEGFHFLHPELFNFLFSYKKGLFVYLPVTLVSLGGIYFLWKKDRFRG
                     LFAAVFLTGLIYVLSSWWMWFYGGSFGTRVIIEYLPVFALLLFYLLDGIKMKPVKISV
                     VSLLVVLTLFCQFQTLQYRYYIIHWSEMTKEKYWDVFLQWKK"
     gene            complement(3283..3915)
                     /locus_tag="VFU15_14185"
     CDS             complement(3283..3915)
                     /locus_tag="VFU15_14185"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005777720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEU4718988.1"
                     /translation="MQVLVGKKAPVFRASAVINGKEIVDSFSLDQYLGKKYVVFFFYP
                     KDFTFVCPTELHAFQEKLKEFESRNVAVVACSTDTEQSHWGWLQVSKDNGGIKGITYP
                     IVADTNKTISYNYGVLNGSYDATEDGEMVASSEMIAYRGLFLIDKNGVVRHQLINDLP
                     LGRNVDEAIRMVDALQFNEENGEVCPANWVKGRSGMKATHTGVAEYFSSN"
     gene            4123..4593
                     /locus_tag="VFU15_14190"
     CDS             4123..4593
                     /locus_tag="VFU15_14190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013930131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEU4718989.1"
                     /translation="MSITVGQPAPDFKLPNTEKEMITLSQFKGKNVLLLFFPFAFTGT
                     CTKELCGVRDDIARYSRANAEVFGISIDTPFSLKRFKEDQSLPFNLLSDFNKTAIHDY
                     DTAYAEFSVGLKGVAKRSAFVIDKEGIVRYAEVLENAGEIPDFTKINAVLEGLN"
     gene            complement(4645..6396)
                     /locus_tag="VFU15_14195"
     CDS             complement(4645..6396)
                     /locus_tag="VFU15_14195"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU4718990.1"
                     /translation="MKRSLSILISVFFFWGFVAASNTILIGLFKKNFELTQFQSQLVD
                     LAFYAAYGIGSLIYFIISFRAGDPLNRIGYKKGLITGLIISAIGALGFIPAEMQESFP
                     LMLASLFVIGLGFALQQIVANPYVIALGDPATGSHRVSLAGGINSFGTTIGPLLLAFA
                     VYGSFSGNSASIEMNGKKTDFPASIERTTGQSETVTATITSDGAANLRDKDYYLLFAD
                     KHYNFFSPVYASMIPSKRGGIILAFSNDSAGDAAVARLKKQFPGVRIPILRVNMETAK
                     KIMSASAPPVVTLKYYGVSAVVKPSIILAVAFLAFALILGLSNLPTVTNSEKLSRDLG
                     ALRYPQVLLGMAAIFIYVGTEVTIQSNLSEFLRRTFGREPGTTVHFISLYWGSLMIGR
                     WVGALTVFNLKKTMRSIMTVVVPVIAYGVILLVNFIKGSPMDELLLYIPFIVLIIIGF
                     FIAAEKPARTMLLFGIMAAGMMAAGLVLQNKWSAYFFVAGGLFCSVMWPCIFSLSIAG
                     VGKYTSQASSLLIMMILGGSLIPPFQGFLSDQIGIHASYIVPLCGFLYLAYFGWRVRR
                     VLRRQNIDYDAQEFSSH"
     gene            6605..>6979
                     /locus_tag="VFU15_14200"
     CDS             6605..>6979
                     /locus_tag="VFU15_14200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718991.1"
                     /translation="MKLNLQNIALSAFAAALILSSCSNNGGDGKTGDTTPPETLSVTG
                     VKIKGQSFLMPSPMIIASIIKKSGALYDKNMLNPTASLPNYGDATKAALNLGVYGADL
                     GYITMYENTGDAMEYYKTVVQLG"
BASE COUNT         1789 a         1773 c         1880 g         1537 t
ORIGIN      
        1 gccggcggga acatgtggct cggctgctac ggagcgggcg tgctctgcat ttccccggat
       61 gaaaaaataa cggtgtacga cgacagcacc gggttgccca cgaacaatgt cctcacactg
      121 ttccaggatg cgaaaggaaa tacctgggtg ggcacattgc agggcggcgt ggtgaaaatc
      181 gctcccggcg ggaaattttc ttacttcacg aaaaaggacg gtctcggtga caattccgtc
      241 tggtccatca cagccgacgg cctcggaaat atttttttcg gaacaaacga caacggcatt
      301 tcctgctacg acggaaaaca gttcgtgaat atttccgcga aggaagggat cagctccgat
      361 cttgtttatg cgctcgtcgc cgacgacaag aaccgcctct gggtgggaac ggacaaaggc
      421 gtgaaccggg tttcgttcgg aaaaaatttc agcattattt ccattaaata tttcggtgaa
      481 agcgacgggt tgcgcgggac cgagatcagc cagaacgcgc tgtacatgga tgcgtcggat
      541 aacatctgga tgggaaccaa tgaaggattg atccgctacc agccgaaata cgattacatc
      601 aacgacaacc cgccgcgtct cgagctcacg gccatccgtc ttttttacca ggccgccgac
      661 tgggcgaaga tgtcgttcaa agttgatccg cgcacgaatc ttcccatcga cccggaattt
      721 tcctggaagg acaatcacat cacgttcgat ttccaggcgc tgaccaccga taacgtgaaa
      781 taccagttca tgctcgaagg tctggatgcg gactggtcgc cgccgaccac gacaacatct
      841 gccgtataca cgaacatccc gccgggaaaa cattacgtgt tccgtgtgaa agcggtgaac
      901 cgcgacggat tctggagcaa ggatgtgctc gcgtattcgt tcgccgtgaa tcctcctttc
      961 tggcagacgt ggtggttcta taccattatc gtcgttcttg ccgcggcttc tattttcgct
     1021 ttcatccgct ggcggaccgc gcgtctcgaa aaagaaaaga aagttctcga agaccgcgtg
     1081 caggaacgta cgcaggagct gcgcgtggcc aacgaccagc tttcactggc gtatacggat
     1141 atcaaggaca gcatcaatta tgcgcagcgc atccagcagg cgatacttcc gctgggcggt
     1201 gagatcagca aggtgcttcc cgatcatttt attttcttcc gtccgcgcga cgtcgtcagc
     1261 ggggattttt actggtttta cccgaaaggc gacaaggttt tcttcgccgc ggtcgattgc
     1321 acgggtcacg gcgtgcccgg tgcgttcatg agcattatcg ggaattccct gctgcacgag
     1381 atcatgaacg aaacagaaac gccttctccc gccgagatac tcaaccggct tcgcgaaaag
     1441 ctcatcgcgg cattgcggca gaaaggcgcc gatgcggaaa gcaaggatgg catggacatg
     1501 gtgctttgct gctacgaccg caaaggatcg aagcttacgt tcagcggcgc aaacaacccg
     1561 ctgtaccttg tttccggcgg aaagctcgag gaattcaaga acgacaagca gcccatcggc
     1621 gtctacggcg atgagatgaa accgttcacg cagaaagaga tcacggtcgg caagggcgat
     1681 gtcatttaca ttttcagcga cggttacccg gatcagttcg gcggcgagaa aggaaagaaa
     1741 ttcctgtata cacgcttcaa gcagatgctg gttgaaatag caccgctcgc catgagccag
     1801 caggaccaga agctcgaaga gaatttcagg aaatggaaag gtgcgaacga ccaggtggac
     1861 gatgttctcg tgatgggatt ccgcttctga tctttatttt ttccattgca ggaaaacgtc
     1921 ccagtatttt tccttcgtca tttccgacca gtgaatgatg taataacggt actgcagcgt
     1981 ctggaactgg cagaacagcg taagcacgac gagcagggag accacggaaa ttttcaccgg
     2041 cttcattttt attccgtcca gcaggtagaa caggagcagg gcgaaaacgg gcaggtattc
     2101 gatgatcacg cgtgtgccga agcttccgcc gtaaaaccac atccaccagg aagagagcac
     2161 gtagatcagc ccggtaagaa atacggcggc gaatagtccg cggaaacggt cttttttcca
     2221 gaggaaataa attccgccga gcgaaaccag cgtcaccggc aggtaaacga aaagaccttt
     2281 tttgtagctg aacaggaagt tgaacagttc cgggtgaagg aaatggaaac cttcttcgct
     2341 gtaagtatcg atgaaaaagc tcccggtgga aagcttgtaa agcattaatt gcacaaagac
     2401 gggaagagcg atcagcagca ggcacagaaa aagcatcaat ggttcgcgca gcaacgcagc
     2461 ttttttcttc agcaggggaa ggaaaccttt cgcttcgaaa agcagccagg cagcgaccag
     2521 cacatttacc ggccgcagaa tggcaatgag tccgaacgcg aggcacgcca ttgccgcatg
     2581 tttcctgttc gccgtgtcga tccagctttt gccgtaaaaa aggaacaggc tcaccatcgc
     2641 gaatgaatac acatgcgaca tgcacggctc cacgatggag taataaaaaa ggtttgtgcc
     2701 gaaataaata acggcgagaa taaatgccga ttgtccttca ttcgccccgt gcgcacgcag
     2761 gaatttccgc agaaaaacga gtccggtccc gaggtagaac agcgccccga taccggtgaa
     2821 gatcacgtac catttcgagt aaccgtcggc cggatccccg ctgaggagcg tcatcgcgtg
     2881 accggccagg aagaagggcg cctgcagcac cgccacgccg cagaaatatt tatcggtcac
     2941 ttttccgttg acgcccgtgc ggtaatcgta tttcgtgtgc tcgtcgtaat aattgctttc
     3001 gatcgtatcg aaaaaagaaa agttaagatc gtggtaaatg aaaatggcgg ggagatacgc
     3061 gtagtagcct ttcccgtccg acgtgatcac gtatttccgg tgctgcgctc cccagtttac
     3121 gttcgcggtc acccaaacgg aaacgagaag aataagcgga acggtaaaac ggctgagcgc
     3181 tgtggaaatt tttttcaccc gggtaaaagt acgacgatga caggcaaaaa aagaaaagcc
     3241 gggaagtaac tcccggctgt atgctttcaa agatgccgtc gctcagttcg aggaaaaata
     3301 ttccgcaacg cccgtgtgcg tcgctttcat gccagatctt cctttcaccc agttcgcggg
     3361 gcacacttca ccgttctctt cgttgaactg gagcgcatcc accatgcgga tcgcttcgtc
     3421 cacgttcctt ccgagcggaa gatcgttgat gagctggtga cgcaccacac cgttcttgtc
     3481 gataaggaaa agtccgcggt acgcgatcat ttcagacgaa gcgaccattt ccccgtcttc
     3541 cgtcgcatcg tacgaaccgt tgagaacacc gtagttgtag gagatcgtct tgttcgtatc
     3601 ggcaacgatc gggtacgtga tgcctttgat cccgccgtta tctttcgaaa cctgcagcca
     3661 gccccagtgc gactgttccg tgtcggttga gcaggcgacg acggcaacgt tgcgtgattc
     3721 gaactctttc agcttttcct ggaaggcgtg aagctcggtc gggcagacga acgtgaaatc
     3781 tttcgggtaa aagaaaaaca ccacgtattt ctttcctaaa tactgatcga gcgagaatga
     3841 atccacgatc tctttcccat tgataacagc gctggctctg aagaccggcg ctttttttcc
     3901 aactaatacc tgcatagtct gttgactgtt ttatggtttg acaatgaaat aaaatttacg
     3961 tttgaacggc acaaaaataa gggctattcc ggcccggcaa ttgacgggga tcatagcaat
     4021 tattgatagg atcatagacg gtttgaattt tcttcatttg tacagcgtgg atgcaattcc
     4081 gaaatttgta cgcattaatt gtttcactaa aaaaatcaag ccatgagcat taccgtcgga
     4141 caacccgcac ctgatttcaa acttcccaac acggaaaagg agatgatcac gctcagccag
     4201 ttcaaaggaa agaacgtgct gctcctgttc ttcccgttcg cattcaccgg cacctgcacg
     4261 aaggaattgt gcggcgtgcg cgacgacatt gcacgttact ccagggccaa tgcggaggtt
     4321 ttcggcattt ccatcgatac gcctttttca ttgaagcgtt tcaaggagga ccagtcgctg
     4381 cctttcaacc tgctttccga tttcaacaaa accgccattc acgattacga tacggcttat
     4441 gctgaattca gcgtcggact gaaaggcgtt gcgaagcgct cggcattcgt catagataaa
     4501 gaagggattg tacgttatgc ggaagtgctg gaaaacgcgg gtgaaattcc cgatttcacg
     4561 aagatcaatg ccgtgctgga aggattgaac tgatcgccac gcgacacgga aaagaacgga
     4621 agctgtgcga acggtttccg ttttttaatg tgaagaaaat tcctgcgcat cgtaatcgat
     4681 gttctgcctg cgaagcacac ggcgcactct ccatccgaaa taggcgaggt aaagaaatcc
     4741 gcagagcgga acgatgtacg acgcgtgaat gccgatctgg tcggaaagga aaccctggaa
     4801 aggcggaatg agcgaaccgc cgaggatcat catgatgagc aggctcgacg cctgtgaagt
     4861 atatttcccg acgcccgcaa tggaaaggct gaagatgcac ggccacatga cggaacagaa
     4921 aagtccgcct gccacgaaga aatacgcgct ccatttgttc tgcagcacga gcccggcggc
     4981 catcatgccc gccgccatga tgccgaataa aagcattgtc cgtgccggtt tttccgcagc
     5041 gatgaaaaag ccaatgatga tgagcacgat gaacgggatg tacaggagaa gctcatccat
     5101 gggacttcct ttgataaaat tcaccagcag gatgacgccg taggcgataa caggtacgac
     5161 tactgtcatg atgctgcgca tcgttttttt taggttgaaa accgtcagcg cgcccaccca
     5221 gcgaccgatc atcaggcttc cccagtaaag agaaatgaaa tgcacggtgg ttcccggctc
     5281 gcgcccgaac gttctccgca ggaattcgga gaggttgctc tggatcgtga cttccgttcc
     5341 cacatatatg aagatcgccg ccatgccgag cagcacctgc ggatagcgca aagcgccgag
     5401 gtcgcgcgaa agtttttcgc tgttcgtcac ggtgggcaga ttggaaagac cgaggatgag
     5461 cgcaaaagca aggaacgcga cagccaggat gatgctcggt tttaccacgg cgctcacgcc
     5521 gtaatatttt aaggtaacca cgggaggcgc agacgcagac atgatcttct ttgccgtttc
     5581 catgttaacg cgcaggatag gaatgcgcac accggggaat tgttttttca atcgggcgac
     5641 agctgcatca ccggcagaat cattggagaa cgcgaggatg atgccgcctc ttttgctcgg
     5701 gatcattgag gcataaacgg gactgaagaa attgtaatgc ttgtcggcga aaagaaggta
     5761 ataatccttg tcgcggagat tggcggcccc gtcgctcgtg atcgttgccg tgacggtttc
     5821 cgattgcccg gtagtacgtt cgatggaagc gggaaaatcg gtcttcttcc cgttcatttc
     5881 aatggaagcg gaatttccgg aaaagcttcc gtaaacggcg aaggccagca gcagcggacc
     5941 gatcgtcgtc ccgaacgaat tgatgccgcc ggccaggctc acgcggtgag agccggtggc
     6001 aggatctccg agcgcgatca cgtacgggtt cgccacgatc tgctggagcg caaagccgag
     6061 gccgatcacg aagagtgacg ccagcatgag cgggaacgat tcctgcattt ccgcgggaat
     6121 gaaacccagc gcgccgatgg cggaaatgat aagcccggtt atcagtcctt ttttgtagcc
     6181 gatcctgttg agcgggtcgc cggcgcggaa ggagatgatg aaatagatga gcgagcctat
     6241 gccgtatgca gcgtagaagg caagatcgac cagctggctc tggaactgcg tcagctcgaa
     6301 attctttttg aaaaggccga tcaggatcgt attcgatgcc gccacgaacc cccagaaaaa
     6361 gaaaacggaa atgagaatgg acagggatcg cttcatcgtt cggttggttt tggccaaaag
     6421 taaaagaatc ccggaacctt gtttgatccg gctttatgcc tgccttaagt ttttagtccg
     6481 aacagttttt tcggcggata aagcctgctt ttgtggagat taagtaaaaa ttaccttttg
     6541 aaaagccggt ttttttttct tacttgcggc gtctttttaa aacctgaaaa cgctgtataa
     6601 ttcgatgaag ttgaatctcc agaatatcgc gctgtcggcc tttgcggcag cgcttatcct
     6661 ttcgtcgtgc tccaataacg gcggcgatgg aaaaacgggc gacacgacgc ccccggaaac
     6721 gttgtccgtt accggcgtaa agatcaaggg ccagagcttc ctcatgcctt cgcccatgat
     6781 catcgcttcc atcattaaga agagcggcgc gctgtacgac aagaacatgc tcaaccccac
     6841 ggcaagtctt ccgaattacg gcgatgcgac gaaagcggcc ctgaacctcg gtgtttacgg
     6901 cgccgatctc ggttacatca cgatgtacga gaatacgggc gatgcgatgg aatattacaa
     6961 aacggtcgtc cagctgggc
//
LOCUS       DASTSF010000494         8853 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_29409,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000494 DASTSF010000000
VERSION     DASTSF010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 8853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8853
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29409"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(291..947)
                     /locus_tag="VFU15_14205"
     CDS             complement(291..947)
                     /locus_tag="VFU15_14205"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEU4718992.1"
                     /translation="MKSLWGNILLVAAAAGLITAGIIIFKKGTNVRTLEYWDPVEEGL
                     RHDQQAGQHHKVLDFSLTDQTGKTITRADFTKSITVVDFFFTTCQGICPRMNTQMQRV
                     YQAYKGNPEVKFISHTVNPENDSVPVLAAYAQRFHADADQWHFVTGDKPQLYNLARKS
                     YLISDTEGDGGKEDFVHSQMFSLVDKEGYIRGIYDGTDPEEVDRLITDIKVLMGQYAK
                     "
     gene            1115..1936
                     /locus_tag="VFU15_14210"
     CDS             1115..1936
                     /locus_tag="VFU15_14210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718993.1"
                     /translation="MNELWVMVHYRENFTLTLHATILTFLILAGCGNTGLQLRNSGQS
                     SRPSFLVPFTGDSVSIRREPQSTFSGSDTGVTGTYNYINRLRCVKTMQLKIRPEYFST
                     DTLVKMSYDDYKIIYREDPPREFEDGNFYFYALLDTAIHPGYYSVITIFDGDKPYSSS
                     TDLELNISFINRVTGRSETISVADYSRSDFSGNDYYASHSSSFFVSPDELQCDDYYRL
                     PNDNFNDDKKPSRKNYRSVVSHYSFREVISADGHINQTHMEFRDVKTVDDSKMWQ"
     gene            2109..4964
                     /locus_tag="VFU15_14215"
     CDS             2109..4964
                     /locus_tag="VFU15_14215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718994.1"
                     /translation="MQNYLFALSLLFTFRLFAQTDSTADPLQSMVLSICSKVENENRL
                     AGNMDPDSTGNLPFGIVKEIGVTRYIIAVDSAKFRTPGATFSAYMALEFPGSAQKICF
                     AAQNVAFNPKGVVPGPNTRLMLVSEHRISLGPKVSLVLRPDGFNYVEWDCNGFEAVNL
                     KGYFEFDPGMIFPDPEFSSDSVVRCSFQLHTNDIHNFIAQLSFPPFCIRGADDLSFTA
                     TDATADFSELSNAPNMVFPAGYNLAGCNGDPLMWTGFYIRQFRVKLPRELAKNGTRPE
                     IIATNLLIDNSGVSGFFQAENLFGTSGGSMNGWGFSLASIGAGFTSNHLNGGNMSGYL
                     KLPLSETDSIAFSAALSENPQTHTIDYSFSVSPANDMHADVLSANLTLSNSSLITVTK
                     RNGILYPVAQLNGTAAFGSTNATTEALSFQSLTIASEAPVLRSGTFSLSSSHPESNSL
                     GGFGFSLNSVTIGQLNGAPAIRFGAAVNFMDPGSLDIGVSGGFTIVTKTTSVPDEATG
                     NTRTRLKWEPDHVSVDNINVAFGSGPFSLNGTIQFRENDPVYGKGFSGQLAFAIADVM
                     PSPASSSVWFGKVNNLRYFYVDAAAPVIIPFSGASIYRLMGGVYYHMHRNSATPLESQ
                     LYTPAFGAASAYVPDANTSLGIRAGVTLGTSGSPDACNGDVALEMNFNTNGGIAQLSL
                     SGTVFFMTGINERLNKPVSQLPATASMLMQYDFNNHALHAVMGAQIHLPSIAATGTAL
                     LHYDPQTWYVYVGRPQQRVNISLLNIGNLSSYIEAGTQLDPIPPPPPQVMSVVNMNNL
                     TGQRNTQALEGAGGFAFGASFGTGFNGNVGFEHFGLYYGINAGAGFDIMVMNYGPHAH
                     CANTTNVIGFNGWYATGQVYAYLQGLAGVQGEVAHQPFNVTILNLSAAAVLQARLPDP
                     GWVGGAVGCDYEILGGLLSGHVDFSFEAGSECTIVGG"
     gene            5019..5402
                     /locus_tag="VFU15_14220"
     CDS             5019..5402
                     /locus_tag="VFU15_14220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009581215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HEU4718995.1"
                     /translation="MKKENFDNELTGKIIGCAMKVHSALGPGLLESSYEECLKYELES
                     NTISVRRQAPLPLVYNGIHLNAGYRVDLLIEKKIIVEIKSVEVLHDIHVAQLLTYLKL
                     SGCRIGLLVNFNVIRLKDGIRRYVI"
     gene            5414..7492
                     /locus_tag="VFU15_14225"
     CDS             5414..7492
                     /locus_tag="VFU15_14225"
                     /inference="COORDINATES: protein motif:HMM:NF012271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HEU4718996.1"
                     /translation="MNLREITSVNAAGDLCVRKPAPIPSFLIAAFLLFSSSLKAQSHL
                     FVKTFFAGDSVMIRWVPGDAETWQLGNKNGYIIERFAANDYADTATRGFTVTPSPVFP
                     LAQNDSAWTTLIAHEPAAAIVFSNLFESKTPSPDPQKRKTEESLSFGFTMKACDISVA
                     TAKAAGLFFIDKNVPPGRYVYRARIAGNKKTISGKAVANKTLSVLLPPDSLRGEFHGK
                     KASLAFMITSTHEQYAGYIIERSADSIHFSRVNTILLSVVRSQYEQDKNTLMYRDTLP
                     EFNRTYWYRVRGRSFFGFDGPPSAMIRGKGKEDMTVFPVMDTLYSPDNKTARLCWHLD
                     SMETREDISGFAVLRAPSVDGPYAPASPQIISPHMFTFTDSFPSASSYYVIAVISMEN
                     DTGFSFPYLLQLRDETPPPVPMHLSGSIDTNGIARLEWENVAAPDLKGYRVFRCNTLN
                     EEFSEVSDSILAQNFFTDTVTMQTLTRTIYYSVRSVDTHYNNSANSDPLLLKRPDKIP
                     PVAPVAKFFFHTDSTIRIGWINSSSDDVMCVELERVCGSSGIPLGSWTGRDTVCCFTD
                     AGVIPGETYSYMITVTDSSGNESRLVFPPVLFQPHIYPALKNFTAVADLASRTITLNW
                     RADDFPVDRYIIYKAKNNDALRTWKTVDGKTLHLADRELYPGNTYNYCIKAVLKNGSE
                     TKTISVSLLF"
     gene            7604..8014
                     /locus_tag="VFU15_14230"
     CDS             7604..8014
                     /locus_tag="VFU15_14230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718997.1"
                     /translation="MKNILFSLAIFILPLAGYAQHDSDSDGDFFASTSTFSFYSYAVH
                     TGGDSASDAVKITRPCQPAFSCSETSRTILVNQSTGSSASAMEVIDKNGKVISFINSP
                     AESEMIPVPQGGEYTVKLIYADGKTRSEKVTVSQ"
     gene            8231..8593
                     /locus_tag="VFU15_14235"
     CDS             8231..8593
                     /locus_tag="VFU15_14235"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEU4718998.1"
                     /translation="MQLSGTRRTLVLIRNGEVTEVRGDRFSIGGSYSDAEKKFTERVV
                     TVQSGDMLYLFSDGYADQFGGPAGKKFMYRNFLRLLKKVSSLSVDEQKSEPGKTFDEW
                     KRDLEQVDDVLVVGIRIT"
     gene            complement(8590..>8853)
                     /locus_tag="VFU15_14240"
     CDS             complement(8590..>8853)
                     /locus_tag="VFU15_14240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4718999.1"
                     /translation="WPYVVAGSQYDIDLGNHGFGWFGVHAGAGYTFLLPYFELGLELR
                     VQRGFAQTNFYDTPQYFLTVTFSDHSDVKDDFSMKKDETENGF"
BASE COUNT         2077 a         2481 c         2218 g         2077 t
ORIGIN      
        1 aggttcacag agaacggtac atgacgaacc tgagactgaa aatattattt taaccacaaa
       61 ggacacggag gctatcacag aggttcacag agaacggtac atgacgaacc caaaactgaa
      121 aatatttttt ttaaccacaa aggacacgga ggctatcaca gaggttcaca gagaatgata
      181 catgccaaac ccaaaactga aaatattttt ttaaccacaa aggacacgga ggtcgtcaca
      241 aaggttcaca gagaaagata aatgaccaac gcgaaactga aaacccggaa ttacttcgcg
      301 tactgcccca tgagaacttt gatatccgtg atgaggcgat ccacttcttc aggatccgtt
      361 ccgtcgtaaa tgccgcggat gtagccttcc ttatcgacaa gcgagaacat ctgcgagtgc
      421 acgaaatctt cctttccgcc atcgccttcc gtatccgaga tgagatagga tttccgcgca
      481 agattataca gctgcggctt gtcgccggtc acgaaatgcc actggtccgc atcggcgtgg
      541 aaacgttgcg catacgcggc gagcacggga accgaatcgt tttcaggatt gaccgtgtgc
      601 gaaatgaatt tcacttcggg gttcccttta tacgcctggt aaacgcgctg catctgcgtg
      661 ttcatgcgcg gacagattcc ctggcacgtg gtgaaaaaga aatcgacgac ggtgatgctt
      721 ttcgtaaaat cagcgcgggt gatcgttttt cccgtctggt ccgtcagcga aaaatcgagc
      781 accttgtgat gctggcccgc ctgctggtcg tgccgcagtc cttcctcgac cgggtcccag
      841 tattcgagcg tgcgcacgtt cgttcctttt ttgaaaatta tgatcccggc ggtgataagc
      901 ccggccgcgg ccgcaaccag aagaatattt ccccacaaac ttttcatggt gcaaaattac
      961 gggctttgcg acagccgcaa ctgttaaatg tcaatgtccg gcaaaaaagc agccgaagtc
     1021 acctccccgg ggtttatagg gtagatggac ggacaggtga caattacacg acggttccgg
     1081 acacttatcc ggtccgggcc tttcctttcc ccgcatgaac gagctttggg tcatggtcca
     1141 ttaccgggag aatttcacac tcacgttgca cgcaacgatt ctcacctttc ttatcctggc
     1201 aggatgcggg aatacggggt tgcagctgcg caattccggt caatcgtcca ggccctcttt
     1261 ccttgtcccg ttcaccggcg attccgtttc cattcgcagg gaaccgcaga gcacattttc
     1321 cggcagcgat acgggcgtga ccggcacata caattacata aacaggctgc gttgcgtaaa
     1381 aacgatgcag ctgaagatcc ggccggaata tttcagcaca gatacgctgg tgaagatgag
     1441 ctacgatgat tataaaatca tttaccgcga agacccgccg cgggaattcg aagacgggaa
     1501 tttttatttc tatgcgctgc tggatacggc gatccatccc ggctactatt cggtcattac
     1561 gattttcgac ggagacaaac cttattcatc ttcgacggac cttgagctga acatctcgtt
     1621 catcaaccgc gttaccggca ggagcgaaac aatttccgtg gccgactatt cccgttcgga
     1681 tttttcaggc aatgattatt acgcttcaca cagctcgtct ttctttgttt cgcctgatga
     1741 gctgcaatgc gatgattatt accgcctgcc gaatgacaat ttcaatgatg acaaaaaacc
     1801 ttcccggaaa aattaccgct cggtggtttc acattattcc ttccgcgaag tgatctctgc
     1861 ggacggtcat atcaaccaga cccacatgga attccgcgac gtgaaaaccg tggatgattc
     1921 gaagatgtgg cagtgaattt cattcttaat tgctgcccgt ccccgatccg accttaaaca
     1981 tttgaaaacg ttttaagtgt tgcggatttt tttcttttgc ggccggaatt acttttcgct
     2041 ggattgccgt gaaatttttt gcggcaaaaa cagcacaaca gcttcttccg gaattccacg
     2101 gtgcgcccat gcagaactat ttatttgctc tctcccttct tttcaccttt cgtcttttcg
     2161 ctcaaactga cagcacggcc gatccgctgc aaagcatggt gctttccatc tgctcgaaag
     2221 tggaaaacga aaaccgtctc gccgggaaca tggaccccga cagcacgggg aatctcccgt
     2281 tcgggatcgt gaaggagatc ggcgtcacgc gttacatcat cgctgttgac agtgcgaaat
     2341 tccgaacgcc gggcgccacc ttttccgctt acatggcgct ggaatttccc ggctccgccc
     2401 agaagatctg cttcgccgcg cagaacgtcg cgttcaaccc gaaaggcgtt gtgcccggac
     2461 ccaacacacg tctaatgctg gtcagcgagc accgcatttc gctcggaccg aaagtttcgc
     2521 tggtgctcag gccggacggg ttcaattacg tcgaatggga ctgcaacggt ttcgaagccg
     2581 tgaacctcaa aggctatttc gaatttgatc ccgggatgat ctttcccgat ccggaattct
     2641 cttccgactc cgtggtgcgc tgctcttttc aattgcacac gaacgacatt cacaatttca
     2701 tcgcgcagct ttcttttccg cctttctgca tccgcggcgc tgatgatctt tccttcacgg
     2761 ccaccgatgc aacggcagac ttcagcgagc tgtcgaacgc accgaacatg gttttccctg
     2821 ccggttataa cctggccggc tgcaacggcg atccgctgat gtggaccggg ttttacatcc
     2881 gccagttccg cgtaaagctc ccgcgcgagc tcgcgaagaa cggaacacgt cccgagatca
     2941 tcgccaccaa cctgctcatc gacaactcgg gcgtttccgg ttttttccag gcggaaaacc
     3001 tgttcggcac ttccggagga agcatgaacg ggtggggatt ttccctcgcg tccatcggcg
     3061 cgggattcac atccaaccac ctcaacggcg gcaacatgtc cggttatctc aaattaccac
     3121 tgagcgaaac cgattcgatc gcgttcagcg cagcgctgag cgaaaatccg cagacgcata
     3181 cgatcgatta cagcttttcc gtttcaccgg cgaacgacat gcacgcggac gtgctttccg
     3241 caaacctcac gctatcgaac tcttcgctca tcaccgtcac gaaacgaaac ggcatccttt
     3301 atcctgtcgc gcagctcaac ggcacagctg cgttcgggag caccaacgca accaccgaag
     3361 cgctttcctt tcagtcgctc accatcgcat cggaagcgcc tgttcttcgc agcggaacat
     3421 tttcactttc ctcttcccat cccgaaagca attcactcgg cggatttggt ttctcgctga
     3481 attccgtcac gatcggccag ctgaacggcg caccggccat ccgtttcggc gcagccgtga
     3541 atttcatgga tcccggctcg ctcgacatcg gtgtgagcgg cggattcacc atcgtcacaa
     3601 agacaacctc cgttccggat gaagcgaccg ggaacacgcg caccagactg aaatgggaac
     3661 cggatcatgt cagcgtcgat aatatcaacg tcgctttcgg cagcggtccg ttctcgctga
     3721 atggcacgat acagttccgc gaaaatgatc ccgtatacgg aaaaggattt tccggccagc
     3781 ttgcatttgc catagccgat gtgatgccgt cgccggcttc atcttccgtg tggttcggga
     3841 aagtgaacaa cttgcgttat ttctacgtcg atgcagccgc gcccgtgatc attcccttct
     3901 ccggcgcttc gatctaccgg ctgatgggcg gtgtttatta ccacatgcac aggaattccg
     3961 ccacgccgct ggaaagtcaa ttgtacacgc cggctttcgg cgctgcttcc gcctatgttc
     4021 cggatgcaaa cacttcgctc ggcatcaggg ccggtgtcac gctcggcacc agcggatcgc
     4081 ccgatgcgtg caacggtgac gtcgcgctgg aaatgaactt caacacgaac ggaggaattg
     4141 cgcagctgtc cctgtccgga actgtttttt tcatgacggg catcaacgaa cggctgaata
     4201 aaccggtgag ccagcttccg gccacggcgt cgatgctgat gcaatacgat ttcaacaatc
     4261 acgcgctgca tgccgtgatg ggtgcgcaga tccaccttcc ttccatcgcc gcaaccggga
     4321 cagcgctgct gcattacgat ccgcagacgt ggtacgtgta cgtcggacgt ccgcagcaac
     4381 gggtgaatat ttccctgctg aacatcggga atctttcttc gtacatcgaa gcgggcacgc
     4441 agctcgatcc cattcctcct ccacctccgc aagtgatgtc cgtcgtgaac atgaacaacc
     4501 tcacgggtca gcgtaacacg caggcgctgg aaggtgcagg cggttttgct ttcggcgcca
     4561 gcttcggaac gggattcaac gggaacgtcg ggttcgaaca tttcggtttg tattacggaa
     4621 taaatgcagg tgccggattc gatatcatgg tgatgaatta cggtcctcat gcacactgcg
     4681 cgaatacgac gaacgtgatc ggcttcaatg gctggtacgc gacggggcag gtgtatgcgt
     4741 acctgcaggg acttgcgggc gtgcagggag aagtggcgca ccagccgttc aacgtcacga
     4801 tcctgaattt atctgcggcg gccgttttac aggcgcggtt gccggatccc ggctgggtgg
     4861 gcggcgctgt gggctgtgat tatgaaattc tcggcggact gctatccggc cacgtggatt
     4921 tttcctttga agcaggaagc gaatgcacga tcgtgggggg gtgatttaac acagagaacc
     4981 acagaggttg tttcacagag ctacacagag atcgataaat gaaaaaagaa aatttcgata
     5041 acgaacttac cgggaaaatc attggatgcg cgatgaaagt acattccgcc ctcggtccgg
     5101 gtttactgga atcttcttac gaagaatgcc tgaaatatga gttggaatcc aatactattt
     5161 ccgtacgcag gcaggcgccc ctgccgctcg tttataacgg cattcatctc aacgccggct
     5221 accgtgtgga tctgctcatt gaaaagaaaa tcattgttga gataaaatct gttgaagtcc
     5281 ttcatgacat tcacgtagcc cagctgctga cctacctcaa actttcaggt tgcagaattg
     5341 gtctgctggt taatttcaac gttatccggc ttaaagatgg catccgccgt tacgttattt
     5401 aattctccgc tctgtgaacc tccgtgaaat cacctccgtg aacgccgcag gcgatctctg
     5461 tgtcagaaaa ccagcgccga tcccttcttt tctgatcgca gcatttcttt tgttttcatc
     5521 ttctctgaag gcccaatctc acctcttcgt caaaactttc tttgcgggcg acagcgtcat
     5581 gatccgctgg gtgccgggag atgcggaaac ctggcagctc gggaacaaaa acggctacat
     5641 catcgaacgt ttcgcagcga acgattacgc ggataccgct acccgcggtt tcacggtaac
     5701 accttctccc gtttttccgc ttgcacagaa tgacagcgcc tggacgacac tcatcgcaca
     5761 cgagcccgcc gccgcgatcg ttttcagcaa cctcttcgaa agcaaaacac cttcacccga
     5821 tccgcaaaaa aggaaaacgg aagaatcgct ttccttcggc ttcacgatga aagcctgcga
     5881 catttcggtc gctacagcca aagctgccgg actttttttc attgacaaaa acgtgccgcc
     5941 gggaagatac gtttaccgcg cgcgcatcgc cgggaataaa aaaacgatca gcggaaaagc
     6001 ggtcgcgaat aaaactcttt ctgtcctgct tccgcccgat tccctgcgcg gtgaatttca
     6061 tggcaaaaaa gcgtcgctgg ccttcatgat cacttccacg catgaacagt acgccgggta
     6121 catcatcgaa cgttcggcag acagcattca tttttcccgt gtgaacacca tcctgctttc
     6181 cgtggtgcgc tcgcaatacg aacaggacaa aaacacattg atgtaccgcg acacgttacc
     6241 ggagttcaac cgtacctact ggtaccgcgt gcggggccgg tcgttcttcg gttttgatgg
     6301 tccgccttct gcaatgatcc gtgggaaagg aaaagaagac atgaccgttt tccccgtgat
     6361 ggatacgctt tattcacccg acaataaaac cgcccggctc tgctggcatc tcgattccat
     6421 ggaaacgcgg gaagatattt caggatttgc cgtgctgcgc gcgccatcgg tggacggccc
     6481 gtatgcgccc gcttcaccgc agatcatttc cccgcacatg ttcacgttca ccgattcgtt
     6541 cccgtctgcg tcgtcctatt atgtgatcgc cgtgatcagc atggaaaacg acacagggtt
     6601 ttcttttcct tacctgctgc agctgcgcga tgaaacaccg ccgcccgttc ccatgcatct
     6661 ttccggctcc atcgacacga acgggatcgc ccggctcgaa tgggaaaatg tcgccgcacc
     6721 cgatctgaaa ggataccgcg ttttccggtg caacacgctg aacgaagaat tttcggaagt
     6781 gagcgattcc atcctggcgc agaatttctt caccgacacg gtcacgatgc agacgctgac
     6841 acgaaccatt tactacagcg tgcgcagcgt cgacacccat tacaacaatt ccgcaaattc
     6901 ggatccgttg ctgttgaaac gtcccgataa aattccgccg gtcgcacccg tggcgaaatt
     6961 ctttttccac accgacagca cgatccgcat cgggtggatc aacagcagca gtgatgatgt
     7021 catgtgcgtg gagctggaac gcgtatgcgg aagttcaggc attccgctcg gcagctggac
     7081 cgggcgtgat actgtctgct gtttcaccga cgcgggcgtt attcccggcg aaacgtattc
     7141 gtacatgatc accgttacgg atagttccgg gaatgaaagc cggctcgtgt ttccgccggt
     7201 tctttttcag ccgcacattt acccggcact gaaaaatttc accgcggttg ccgacctcgc
     7261 gtcgcgcacg ataacattaa actggcgggc ggacgatttc ccggtcgacc gttacatcat
     7321 ttacaaagcg aagaacaacg atgccctgcg cacatggaag accgtcgacg ggaaaaccct
     7381 tcacctcgcc gaccgggagc tgtaccccgg aaacacgtat aactattgca tcaaggcggt
     7441 gctgaagaac ggatcggaaa cgaaaaccat ttccgtcagc ctgcttttct gatattttcc
     7501 ctttacagat tttccccaaa tcttacatcc gcaaggacgg gccccgatgc ggcttacctg
     7561 cgcgttttta ccgctctatg tttgctcaac aaataactgg gccatgaaaa acatattgtt
     7621 cagcctcgcc atattcatcc tgccgcttgc aggttatgcg cagcacgact ccgactcgga
     7681 cggcgacttt ttcgcatcga catccacctt ttcattttac tcttacgccg tacataccgg
     7741 cggcgattcg gcaagcgatg cagtaaagat cacgcggccc tgccagcccg ccttttcctg
     7801 ctcggaaact tccaggacca tcctggtgaa ccagtcgacg ggcagcagcg cgagcgcgat
     7861 ggaagttatc gataagaacg gaaaagtcat cagctttatc aattctcccg cggaatccga
     7921 aatgattcct gtaccgcaag ggggcgagta cactgtgaaa ttaatttacg cagacgggaa
     7981 aacacgatcg gaaaaagtca ctgtgagcca ataattcttt tccggtcgtt gaagaaagct
     8041 ctccgcccgg agggcttttt ttgtgccgtt acgggaagga acgataatgg ttcgtcttaa
     8101 aaaatcatct ttaatgtaag caaccggaca tcgtcgtgtg ggaatcatcg acgctcacac
     8161 cgatcttccg cgatgacggc acgctgcaac ggacatcggc ctgctcgtct ggcacatcga
     8221 aaaaggatac atgcagcttt ccggcacacg ccgcacgctt gtgctcatcc gcaacgggga
     8281 ggtgacagaa gtgcgcggcg accgtttttc cattggcgga agctacagcg acgctgaaaa
     8341 aaaattcacg gagcgggtgg tcaccgttca gtccggtgac atgctgtatc ttttttcgga
     8401 cgggtacgca gatcagttcg gggggcctgc gggaaaaaaa ttcatgtacc ggaacttcct
     8461 tcgcttgctg aaaaaagttt cttcgctttc cgtcgatgag cagaaaagcg agccgggaaa
     8521 aacgttcgat gaatggaaaa gagatctcga gcaggtggac gatgtgctgg tggttgggat
     8581 acgaattacc taaaacccgt tttccgtttc atccttcttc atgctgaaat cgtctttcac
     8641 atcggaatga tcggaaaacg ttacggtgag gaaatattgc ggcgtgtcgt aaaaattcgt
     8701 ttgtgcaaac ccgcgttgca cgcggagctc caggcccagc tcgaaatacg gaagcaggaa
     8761 cgtatagccc gcgcccgcat ggacgccgaa ccatccgaaa ccgtgatttc cgaggtcgat
     8821 atcgtactgg cttcccgcga caacgtacgg cca
//
LOCUS       DASTSF010000495         5611 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1899897,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000495 DASTSF010000000
VERSION     DASTSF010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5611
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1899897"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2738
                     /locus_tag="VFU15_14245"
     CDS             <1..2738
                     /locus_tag="VFU15_14245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719000.1"
                     /translation="LFQGFLELIPIIGPAIFDKLVESGLIVRAGAWLDSVLGQLGLSL
                     ASIRDTFSRCWDEMGITHGISGNIAIIHRYFDPIVNRISNFARQVINRVVQFLRETLF
                     TPLNNFLSEIPGWSLFTTILGRNPLTNQAVPRTALNLVRGFMEFIPGGTEKLDQLVQS
                     HALERAYQWFLDETHARNLTWERISATFSRAWDSLDATSILHPIDTFRNLSNIFSPLL
                     RDLLGFAVAALEKILEFIFEAAMGAGGARILSILRRVRSTFLLIIRNPVGFLSNLVTA
                     IGRGVRQFMQHILVHLRNGVIEWLTGGLTRAGVQLPEQWDLRGFLFFVLQILGLTWDN
                     IRQRLVTLLGERTVSLLERGFQFLQEVREKGFVEAIKDRISEFFGNIREMVLNRIKEW
                     IQQRIVMAAITQLLSLLSPVGAVIQAILKTYNTIMFFIERMNQILAFVESIVDSITNI
                     AMGSLGQAANYIEQSMARFIPVMLSFLARFIGIGDIAAPIQRAIQAIQAGVNRALDRI
                     VAWIQTAAQRLMQAGRSAVGAVASWLGLRKEFRTNDHASHSLFVEDHGGRPTLMIASE
                     KQPFSQYLNNLTVPASDSAKRRAKQNAITSHSNISTKLNELKTMEAAPSTPRGNLTAK
                     QNEIRSEYDTLSDSVTVLGIGGSRSETVQTTITPTGGSKLTHIQARPLTALRGNTVGN
                     PNANSVTGVPGWSFLDRINIARGNDNTIYNDWVRWHLIHSSMHGPASIFNLVAAPRVV
                     NTRFAGQVEHPVLRRLLEPGTMLYYDVDIAYGNSAAPLNDFPTSITYVWGTMKMDDNN
                     RPVDDQQIDRKEYNGFPLPAMPTSGGPLTVRLKNIGRPFLQTNLRIPQDRARRIAAAC
                     SAYSSLDVYSAMNTYYALPTSSFNGTPYSNFLSSDKASIVAAQARFAGTYIIDLT"
     gene            2750..3382
                     /locus_tag="VFU15_14250"
     CDS             2750..3382
                     /locus_tag="VFU15_14250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719001.1"
                     /translation="MYDYLGTIQKASQTIHLSPHLVVKEDRIFEGLGTDYISDFLRSE
                     GCDAPEDYIQFCSAVDFAHIEWEVKPDSIRSLGLEASMCTTGRIFIDSIENVFAPGFW
                     LNEIWNEFQHIHQLNVGSRLIPVDYFDPDNSACICYEMKNKKIGPGLFYFDHREGLLP
                     LDISLDAYFEKLLSYRGVYGWQKALLGGNTQYVAQTTSFIKKIFNETISG"
     gene            3417..>5611
                     /locus_tag="VFU15_14255"
     CDS             3417..>5611
                     /locus_tag="VFU15_14255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719002.1"
                     /translation="MSAETTTSSVTPSSKSKSIGKAADTPAPVHTSAAEGGDGKRMYR
                     MSNGQMVELPPDMSAEDAAKLESEAHAAQKKLGKGPPPKPVPDVKAEAKKKTKKEKAK
                     EGKEKSKKKKAAPKHPGAGHKKQAVTHGGGKFHIRGKVGAWLMMKGAPVIARGETKLQ
                     QLKTNEQQHDDAASKLSQTEKAVVPPQTEAQAAGNSGQVNAVDAKPAPQPDEAKAKQA
                     FNDAIQENVPKSIEDVDNFKRDNKAENMSAAASVVVNTDKDNVAGTFGDMKQDPPPAP
                     PAGVPEALPPEEVAPVTPQMNLGANTIAPLQKEHTDVSNYTKEADTKLKDEGVTQEQL
                     DMVDSGDLAEANKEKKGMEKKAKTEPASIQQFAKTENTKVDTDLKHEEQKGRTEIKAH
                     RKQHLAATRQKQQGTKSLMEKKRDEVAKHINDIYKRAQEKVTKKLADLETTAMKRFDE
                     GNAKATHEFEDNVKRELDAFKDERYSGFWGWARHAKDWLLGMDDLPEVKNIFDTNRST
                     FVNKINKLVADITADNKKVIQECKDELAAAKKEIKTYVDKLGPDLKDAGKKAQEEMNG
                     KLDELDKMVAKKEEDLQNKLKDKQQAAIKAIDEKIEKMKEEMSGALSKLANLLLLAAK
                     KFFSWALEKFGFSLAEIESIINKGAAVLKAIFTQPIRFVRNLIDAAITGFKNFGKNFL
                     KHLKDALFEWLTGSLDGVILPQTWDFRGIISVALQMIGISYQNIRKHMVA"
BASE COUNT         1522 a         1487 c         1521 g         1081 t
ORIGIN      
        1 atcttttcca gggattcctc gagctgatcc cgatcatcgg tcctgccatt ttcgacaagc
       61 tcgtggaaag tggtcttatc gtaagagccg gggcatggct cgattctgtt cttggtcagc
      121 tggggctcag cctcgcttcc atacgcgata cgttttcacg ctgctgggac gagatgggca
      181 ttacgcacgg catcagcggg aacatagcga tcattcaccg ttatttcgac ccgatcgtca
      241 accgcatttc aaattttgcg cggcaggtga tcaaccgcgt cgtgcaattc ctgcgcgaaa
      301 cattgttcac accattgaac aatttccttt ccgagatccc gggatggagc ctgttcacca
      361 ccattctcgg tcgcaacccg ctgactaacc aggccgttcc gcgcacagcg ctcaacctcg
      421 tgcgcggttt catggaattc atcccgggcg gaacagaaaa gctcgaccag cttgtgcagt
      481 cgcatgcgct tgagcgcgct taccagtggt tcctcgatga aacgcatgcg cgcaacctca
      541 cgtgggaacg catcagcgcc acgttctcgc gcgcgtggga ttcgctcgat gcgacgagca
      601 tcctccaccc gatcgacacg ttccgcaacc tgtcgaacat tttcagtccg ctgctgcgcg
      661 acctgctcgg cttcgcggtt gccgcactgg aaaaaatcct tgaattcatt ttcgaagcgg
      721 ccatgggcgc cggcggtgcg cgcatccttt cgatcctgcg gcgcgttcgt tccacgttcc
      781 tgctgatcat ccgcaacccg gtaggatttc tcagcaacct cgttacggcc atagggcgcg
      841 gcgtgcggca attcatgcag cacattcttg tgcacctgcg caacggggtg atcgagtggc
      901 ttacgggcgg actcacgcgc gcaggcgttc agctgcccga gcaatgggac ctccgcggat
      961 ttctcttctt cgtgctgcag atactcgggc ttacgtggga caatatccgt cagcgcctcg
     1021 tgacactgct cggggaacgc acggtgagcc tgctcgagcg cggcttccag tttttgcagg
     1081 aagtgcgtga gaaaggattc gtggaagcga tcaaggaccg catcagcgaa tttttcggca
     1141 acatccgcga gatggtattg aaccgcatca aggaatggat ccagcagcgc atcgtcatgg
     1201 ccgcgatcac gcagctgctt tcgctgctga gcccggtagg cgcggtgata caggcgatcc
     1261 tcaaaacgta caacacgatc atgttcttca tcgagcgtat gaaccagatc ctggcgttcg
     1321 tggaatcgat cgtcgattcg atcacgaata ttgcgatggg aagcctggga caggccgcga
     1381 attacatcga gcagtcgatg gcgcgtttca ttcccgtgat gctgagcttc cttgcgcgct
     1441 tcatcggcat cggcgacatc gcagcgccga tacagcgggc gatacaggcg atacaggcgg
     1501 gcgtcaaccg cgcgctcgac cgcatcgtgg cgtggataca gacggctgcg caaaggctga
     1561 tgcaggcggg gcggagcgcg gtgggagcag ttgcaagctg gcttggattg cggaaagaat
     1621 tccgaaccaa tgaccacgcg tcacactcgc tttttgtcga agatcatggc ggccgcccca
     1681 cactgatgat agcaagcgaa aaacaaccct tctcgcaata cctgaacaac cttaccgtac
     1741 cggcttcaga ttccgcaaaa agacgggcta agcaaaatgc aataacaagt cacagcaaca
     1801 tcagcacaaa actcaatgaa cttaaaacaa tggaagctgc gccttccaca cccaggggca
     1861 atcttacggc aaagcagaac gagatacgaa gcgagtatga cacgctgtct gatagcgtaa
     1921 ctgttctcgg gatcggtggc agccggagtg aaaccgttca gacaaccata actcccactg
     1981 ggggtagcaa gttgacgcat atccaggcaa gaccattaac tgcacttaga ggaaataccg
     2041 tcgggaatcc gaatgcgaac agtgttaccg gtgtgccggg atggagtttt ctggatcgca
     2101 tcaatattgc caggggaaat gacaatacca tttacaacga ctgggtgaga tggcatttga
     2161 tacacagctc catgcatgga ccggcaagca tattcaatct tgttgccgca ccgcgggtgg
     2221 tgaatacaag atttgcaggt caggttgagc acccggttct tcgccgtctg cttgagccgg
     2281 gaacaatgtt atactatgat gttgacatcg cttacggcaa ttccgctgcg ccgcttaatg
     2341 atttcccgac gagcatcact tacgtctggg gaacaatgaa aatggatgac aacaaccggc
     2401 ctgtggatga tcaacagatc gaccggaagg aatacaatgg tttccccctg ccagctatgc
     2461 cgacatccgg tggaccgctg accgtgagat tgaaaaacat cggcaggccc tttttacaga
     2521 ctaatctccg cataccgcag gaccgtgcac gtagaattgc agccgcctgc agcgcatact
     2581 ccagtctgga tgtttattcg gcgatgaata catattacgc gttgcctaca tcatccttca
     2641 acggaacacc ttattcgaat ttcctcagct ctgataaagc ttcaatagtt gcggcccagg
     2701 ccagatttgc cggaacatat attatcgacc taacctgaaa gcgatcgcta tgtacgatta
     2761 cctcggcact attcaaaaag cttcacagac cattcatcta tcgcctcatc tggttgttaa
     2821 agaggaccgg atattcgaag gtctgggcac tgactatatt tcagacttcc tgcggtcaga
     2881 aggatgcgat gcccccgaag attatattca attctgtagc gccgttgact ttgcccatat
     2941 cgaatgggaa gtcaaacctg attcgatccg atcgctggga ctcgaagcaa gcatgtgcac
     3001 gacaggccgg atatttatcg attccatcga gaatgttttc gctcctggat tctggctgaa
     3061 cgaaatatgg aacgaattcc agcacataca ccaactcaat gttggcagtc gtcttatccc
     3121 ggtagattat tttgacccgg acaattctgc atgtatttgc tatgagatga aaaataaaaa
     3181 gataggcccc gggctctttt attttgatca cagggaagga ttgcttccgc ttgatatttc
     3241 gcttgatgcc tattttgaaa aattactttc ctaccgcggt gtttacggct ggcaaaaggc
     3301 acttctagga ggaaacacac aatatgtggc ccagaccacc tcgtttataa agaagatatt
     3361 caatgaaact atttccggct aataaataaa gagaatctgc cttgtaaata aaatatatgt
     3421 ccgccgaaac cacaacctct tccgttaccc cgtcttcgaa atcgaagtcg ataggaaagg
     3481 ctgcggacac gccggcgcct gtgcacacca gcgcggcgga aggcggcgac ggcaaacgca
     3541 tgtaccgcat gagcaacggg cagatggtgg agctgccgcc cgacatgagc gcggaagatg
     3601 cggcgaaact cgaatcagaa gcgcatgcag cacagaaaaa actcgggaaa ggtccgccgc
     3661 cgaaacctgt tcccgatgtg aaagcggaag cgaaaaagaa aacaaaaaaa gaaaaggcga
     3721 aagaaggaaa agagaaaagc aaaaaaaaga aagcagcgcc gaagcatccg ggtgcggggc
     3781 ataaaaaaca ggcagtgact cacggcggcg gaaaattcca catccgcgga aaagtcggtg
     3841 cgtggctgat gatgaaaggc gcaccggtga tcgcgcgcgg cgagacgaag ctgcagcagc
     3901 tgaaaaccaa cgagcagcag cacgacgatg cggcatccaa actttcgcag acggagaaag
     3961 ccgtcgttcc tccgcagacc gaagcgcagg ccgcgggaaa ttccgggcag gtgaatgccg
     4021 tcgatgcgaa gccggctccg caacctgacg aagcaaaagc gaaacaggcc ttcaacgacg
     4081 cgatccagga aaatgtcccg aaatcgatcg aggatgtcga caatttcaaa cgcgacaaca
     4141 aagcggaaaa catgagcgcc gctgcgtccg tggtcgtcaa tacggacaag gacaatgtcg
     4201 ctggaacatt cggcgacatg aagcaggatc cgccgccagc tccgccggca ggtgttccgg
     4261 aagcgttgcc gcccgaagaa gttgcaccgg tcaccccgca gatgaacctc ggcgcgaaca
     4321 cgatcgctcc gctgcagaaa gagcacaccg acgtcagcaa ttacacgaaa gaagccgaca
     4381 cgaagctgaa agacgaaggc gttacgcagg agcagctcga catggtcgac agcggggacc
     4441 ttgccgaagc gaacaaggaa aagaagggaa tggaaaaaaa ggcgaagacc gagcccgctt
     4501 ccatccagca gtttgcaaaa acggaaaata cgaaagtcga taccgacctg aagcacgaag
     4561 agcagaaagg acgcacggag atcaaagcgc accggaagca gcacctggcc gccacacgcc
     4621 agaaacagca gggcaccaaa tcgctgatgg agaaaaaacg cgacgaggtg gcgaagcaca
     4681 tcaacgacat ttacaaacgc gcacaggaaa aagtgacgaa gaagctcgcc gatctcgaaa
     4741 cgacggcgat gaaacggttc gacgaaggca acgcgaaagc cacgcacgaa ttcgaagaca
     4801 atgtgaagcg cgaactcgac gcgttcaagg acgaacgtta ttcgggtttc tggggatggg
     4861 cgcggcacgc gaaagactgg ctgctgggca tggacgatct gccggaagtg aaaaacatct
     4921 tcgacaccaa ccgatcgacg ttcgtcaaca agatcaacaa gctcgttgcg gacatcacgg
     4981 ccgacaacaa aaaagtgata caggaatgca aggatgagct tgcggcggcg aagaaagaga
     5041 tcaaaacgta tgtcgacaag ctcggtcccg acctgaaaga cgcaggtaaa aaagcgcagg
     5101 aggaaatgaa cggcaagctc gacgagctgg acaagatggt ggcgaaaaaa gaggaagacc
     5161 tgcagaacaa gctcaaggac aagcagcagg cggccattaa agcgatcgac gagaagatcg
     5221 agaagatgaa agaggaaatg tcgggcgcgc tgtcgaagct cgccaacctg ctgctgcttg
     5281 ccgcgaaaaa attcttttca tgggcgctgg aaaaattcgg cttctcgctg gccgagatcg
     5341 aaagcatcat caacaagggc gccgccgtgc tcaaggccat cttcacacaa ccgatccggt
     5401 tcgtcaggaa tctgatcgac gcagcgataa caggtttcaa aaatttcggg aagaattttc
     5461 tcaagcattt gaaggacgcg ctgttcgaat ggctcacggg atcgctcgac ggtgtaatac
     5521 ttccccagac atgggatttc cgcgggatca tcagcgtagc gctccagatg atcggcattt
     5581 cctaccagaa tatccggaaa cacatggtgg c
//
LOCUS       DASTSF010000496         8802 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_538390,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000496 DASTSF010000000
VERSION     DASTSF010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 8802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8802
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_538390"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1881)
                     /locus_tag="VFU15_14260"
     CDS             complement(<1..1881)
                     /locus_tag="VFU15_14260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014203618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HEU4719003.1"
                     /translation="MLKLNEGYTPRWIVLLIDLFIVAAGISMAYLLRFDFSVPQHEIA
                     AFPVVYSVMIGVRLLSFLIARIPWAIVRYMSQRDAIRILIAISAGTILFYITNQVTHR
                     FITGTYIIPHSIILIEFLATTFGMVSARLIYKAFWTEMKNPSREKRSVIIFGAGESGL
                     ITKRTLDRDAGTKYRVLAFIDDDKAKHGRKLEGVTIYGEEKLPELLKKNDVAQVIISV
                     QQISPLRKQEIVDLCLANNARVLNVPPVSTWIRGELSFRQIRKVRIEELLEREPIRLD
                     EDNIGKQLRGKTILVTGAAGSIGSEMVRQIAKFSPGLLVLLDNAESPLHEMELELSDT
                     MKGLKYEIVIGDVRNRERMENVFRTFHPQIVYHAAAYKHVPMMENNPSESILTNVCGT
                     KNVADLSVQYNAEKFVLVSTDKAVNPTNVMGASKRIAEIYVQSLNNSTGGTRFITTRF
                     GNVLGSQGSVIPRFRQQIENGGPVTITDPEITRYFMTIPEACRLVLEAGCMGKGGEIY
                     IFDMGKSVKIIDLAKKMIQLSGLTLGKDIEIVTTGLRPGEKLYEELLNNKENTLPTHH
                     EKIMIAKVRSYDFAEVSKNVEELIGMFDSQDNMKIVALMKKMVPEYVSNNSVFEKLDS
                     LKK"
     gene            2054..3049
                     /locus_tag="VFU15_14265"
     CDS             2054..3049
                     /locus_tag="VFU15_14265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018489979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEU4719004.1"
                     /translation="MPASLVTGGAGFIGSHVVNDLLRMGHDVIVLDDLSGGFAENVNG
                     KAKFIAGSVTDHLLLEKLFSENKIDYIYHLAAYAAEGLSHFIRRFNYTNNLIGSVNLI
                     NEAVKQDVKCFVFTSSIAVYGALQPPMREDMTPIPEDPYGIAKLAVEQDLRTAHEMFG
                     LDYVVFRPHNVYGEFQNIGDRYRNVVGIFMNQLMQQKPLTVFGDGKQTRAFSYIGDIS
                     PVIAESVNVPAAKNQVFNIGADNDYTVNELAAETMQAMGIRGEIRYLEARNEVLHAHA
                     DHSKVKKVFGRRAITPLSEGLARMAEWAKRAGVRKSKRFGEIEIHEKLPPVWLEE"
     gene            3079..3975
                     /locus_tag="VFU15_14270"
     CDS             3079..3975
                     /locus_tag="VFU15_14270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011583472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU4719005.1"
                     /translation="MVPYKSTPLHPVETAGGTIDSFISGTINADSPTVKSFGDEWNRF
                     SSFTEEEIQRAGEQYFDIVTEEMVNKDTVALDIGCGSGRWSKFLSPRVKFIEAVDPGE
                     AVHAAVTLTKNCGNIRITQAGYRNIPFEKGSFGFVFSLGVVHHLPDTAGAVQEAASLV
                     KKNGWLLLYIYYNLDNRGALYRLLFGLSSVMRRIISRFPRGLKFFVCELIAIFVYGPF
                     VLCARFLKLFGGHSWKKIPLSYYADKPWKVIRNDSLDRFGTPLEKRFSREEIEKMLLD
                     AGMDDIRFSENEPYWHVVSRKK"
     gene            3972..5054
                     /locus_tag="VFU15_14275"
     CDS             3972..5054
                     /locus_tag="VFU15_14275"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015691431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEU4719006.1"
                     /translation="MKRKKILFVASHRPGRAPGQRFRFEQYFDFLRQNGFDCELSYII
                     SENDDKLLYRKGHYLEKFSIHRRSLKKRKNDVLRAGEFDIIFIFREAIVTGSVKFEKL
                     FRKSGAKIVFDFDDAIWHLDVSEANRMFGFLKRPQKTGEIIGLSDLVFAGNNYLAEYA
                     RKFSTNVAIVPTTIDTEEYKAEIPAGKNGKITIGWSGSLTTIKHFEFAVPFLRELKKK
                     HGDKISVKVIGDGTYVNEELGVKGIPWKKEDEVKELSTFDIGIMPLPDDEWAKGKCGL
                     KGLQYMALGIPTIMSPVGVNTEIITEGVNGFLATSTEEWVRKIESLITSEELRRKMGS
                     EARRTVEEKYSVSAWKEKYLELLRSL"
     gene            5232..6314
                     /gene="gcvT"
                     /locus_tag="VFU15_14280"
     CDS             5232..6314
                     /gene="gcvT"
                     /locus_tag="VFU15_14280"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012780583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HEU4719007.1"
                     /translation="MKNTALTAKHVALGAKMVPFAGYNMPVSYTGLNEEHLTVRNAVG
                     VFDVSHMGEFILKGEKALDLIQAVTTNDASQLYDGRVQYSCLPNGKGGIVDDLLVYRI
                     DEKTYMLVVNASNIDKDWNWISRHNSYGVEMKNISERTSLLAVQGPKAQATLQKLTGI
                     NLAEIPYYHFKKGKFAGEDNILISNTGYTGAGGFEIYFDNAVAEKMWDAVFKAGEEFG
                     IKPIGLGARDTLRLEMGFCLYGNDIDDTTSPIEAGLGWITKFNKEFVDKDKMLALKEK
                     GVAKKLVGFEMIDRGIPRHDYEITDANGNVIGKVTSGTQSPSLQKGIGMGYVKTEFSK
                     TGTEIYVKVRDKALKAQVVKVPFLKS"
     gene            6327..7052
                     /locus_tag="VFU15_14285"
     CDS             6327..7052
                     /locus_tag="VFU15_14285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012780584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HEU4719008.1"
                     /translation="MSTETAIRIDACFSPYLYPVYRDEGSIVVIIDILRATSAICTAF
                     HYGAEKIIPVATVEEARALKEKGFLAGAERNAIRVEGFDFGNSPFDYMGEDIAGKTIV
                     LTTTNGTQAIEASKYAHKVVIGAFTNISALCDWLVKQDRNVLLLCSGWKNKFNLEDSL
                     FAGAMTAELVKRNPAYKLGDACLALEYLYQMAKKNPVKFLGRASHKERLSKLNLKDDI
                     RYCLQTDLTTVIPVLEDGALVKM"
     gene            7115..8110
                     /locus_tag="VFU15_14290"
     CDS             7115..8110
                     /locus_tag="VFU15_14290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020595101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HEU4719009.1"
                     /translation="MRKVFRNSVLLFVLLSIVVFSLPQQRAESWGFYAHKRINRMAVF
                     TLPPEMFGFYKKHIEYITEHAVDPDKRRYSVAEEAPRHYIDIDHYGPNCFDSVPRLWK
                     KAVAKYTEDTLNAYGIVPWWIDVMTYRLTKAFKDGDVDRILRLSAELGHYVGDAHVPL
                     HTTENYNGQMTNQVGIHGFWESSVPEMFGDQYDYFVGRAEYIDSPINAAWAIVKVSHS
                     EVDSVLSLEAQLNATTPPDRKYAFVNRGNTTVKTYSEEYTASYSRMLNGMVERRMRAA
                     IHMVGSLWYTAWVNAGQPDLSKLEDKDVSDSAKKAQEQEEYLWKNGKLKVKGHED"
     gene            8220..8552
                     /locus_tag="VFU15_14295"
     CDS             8220..8552
                     /locus_tag="VFU15_14295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719010.1"
                     /translation="MRNYFLIVVPVLAICCIFSGQGILRFIEKSQTISSNAILSTSYD
                     DDSISATAADDDGDDVSETLFRLLPVSLVAVFVYLFLHSVPQASFSLLPHGAPKQVSP
                     VFLGVFRI"
     gene            8609..>8802
                     /locus_tag="VFU15_14300"
     CDS             8609..>8802
                     /locus_tag="VFU15_14300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719011.1"
                     /translation="MIRLPVPKTLLPVMALVVLSFGKTAATEVLKTDTVPLDVNTAEQ
                     RLVSKSLMLLAARYDVDIAKS"
BASE COUNT         2100 a         2440 c         2326 g         1936 t
ORIGIN      
        1 tttcttcagt gaatccagct tctcgaaaac agaattgttg ctcacgtatt cgggaaccat
       61 cttcttcatc agcgcaacga tcttcatatt gtcctgcgaa tcgaacatgc cgatcagctc
      121 ttccacgttc ttcgaaactt cggcaaaatc atacgaacgc actttggcga tcatgatctt
      181 ctcgtggtgc gtgggcaacg tgttctcttt gttgttcagc agctcctcgt ataatttttc
      241 accgggacgc aggccggtcg ttacgatctc gatgtctttt cccaatgtca gtccggaaag
      301 ctggatcatc tttttcgcga ggtcgatgat cttcaccgat ttccccatgt cgaaaatgta
      361 gatctcaccg ccttttccca tgcagccggc ttcgagcacg agacggcacg cctcgggaat
      421 ggtcatgaaa taacgcgtga tctcggggtc cgttatcgtt accgggccac cgttctcgat
      481 ctgctggcgg aaacgcggga tcacgcttcc ctgtgagccg agcacgttgc cgaagcgcgt
      541 ggtgatgaag cgcgttccgc cggtgctgtt gttcagcgac tgcacgtaaa tttccgcgat
      601 gcgcttcgac gcgcccatca cgttcgtcgg gttgacggct ttgtcggtgg aaacgagcac
      661 gaatttttcc gcgttgtact ggacggaaag atccgcgacg ttcttcgttc cgcatacgtt
      721 cgtgagaatg gattccgacg ggttgttctc catcatcggg acgtgcttgt acgccgcagc
      781 atggtaaacg atctgcgggt gaaacgtgcg gaacacgttt tccatccgtt cccggttgcg
      841 cacatcgccg atgacgatct cgtacttcag ccctttcatc gtatccgaaa gctccagctc
      901 catttcgtgc agcggtgatt ccgcattgtc gagcagcacg agcaggccgg gactgaactt
      961 cgcgatctgc cgcaccattt cgctgccgat cgatccggcc gcaccggtga cgaggatcgt
     1021 tttcccgcgc agctgcttcc cgatgttgtc ctcgtcgagg cggatcggtt cgcgctcgag
     1081 caattcctcg atgcgcactt tgcggatctg gcggaagctg agctcaccgc gtatccacgt
     1141 gctcacgggc gggacgttca gcacgcgcgc gttgttcgcg agacagaggt cgacgatctc
     1201 ctgcttgcgc agcggcgata tctgctgcac ggaaataatt acctgcgcga cgtcgttctt
     1261 cttcagcagc tcgggcaatt tctcttcgcc gtagatcgtc acgccttcga gcttgcgccc
     1321 gtgcttcgct ttgtcgtcgt cgatgaaggc cagcacacgg tattttgttc ccgcatcgcg
     1381 gtcgagcgtg cgcttggtga tgagcccgga ttcgcctgcg ccgaaaatga tcacgctgcg
     1441 tttttcgcgc gacggatttt tcatttcggt ccagaacgct ttgtagataa gacgggcgct
     1501 caccataccg aacgtagtgg cgagaaattc gataaggata atggaatgcg ggatgatgta
     1561 ggttcccgtg atgaaacggt gcgtgacctg gttggtgatg taaaacaaaa tcgtccccgc
     1621 tgaaatagcg atgaggatgc ggatcgcgtc gcgctggctc atgtaacgca cgatcgccca
     1681 cgggatgcgc gcgatgagaa agctcagcag gcgcacaccg atcatcaccg aataaacaac
     1741 gggaaaagcg gcgatctcgt gctgcggaac ggaaaaatcg aaacggagca ggtacgccat
     1801 ggaaattccc gcagcgacga taaacaggtc gatcagcaga acgatccagc ggggcgtgta
     1861 accttcattg agctttagca cgaacgtaaa ggtaagaaat ggggtgctga aagaaaccgc
     1921 acacgggaaa ccattggcgg gcacctgtct tcaaacacaa atccccggac cagcccaacc
     1981 ggcgctttcc aactttcaaa ctctaaaaca tttccaaccc ttaacccaaa acttgaaact
     2041 atctttacag cctatgccag cttcactcgt caccggcggc gccgggttca tcggctcgca
     2101 cgtggtcaac gatcttctcc gcatgggaca cgacgtgatc gtgctcgatg atctcagcgg
     2161 cgggtttgca gaaaacgtga acggcaaagc gaaattcatt gcgggttctg tcaccgatca
     2221 cctgctgctg gaaaaactgt tttcggaaaa taagatcgat tacatctacc accttgccgc
     2281 ttatgccgcc gagggactga gccatttcat ccggcgtttc aattacacga acaaccttat
     2341 cggcagcgtg aacctgatca acgaagccgt gaagcaggat gtgaaatgtt ttgttttcac
     2401 ttcttccatc gcggtttacg gtgcgctgca gccgcccatg cgcgaggaca tgacgcccat
     2461 tccggaagat ccgtacggca tcgcgaagct ggccgtcgag caggacctgc gcacggcgca
     2521 cgaaatgttc ggtctcgact acgtcgtttt ccgcccgcac aacgtgtacg gggaattcca
     2581 gaacatcggc gaccgctacc ggaatgtcgt cggcattttc atgaaccagc tcatgcagca
     2641 gaagccgctc acggttttcg gcgacggaaa acagacgcgc gcgttcagct acatcggcga
     2701 tatttcaccg gtcattgcag aaagcgtgaa tgttcctgcc gcgaagaacc aggtgttcaa
     2761 catcggcgcg gacaacgatt ataccgtgaa cgagctcgcg gcggaaacaa tgcaagccat
     2821 gggaatacgc ggcgagatcc gttacctcga agcgcggaac gaagtgctgc acgcacatgc
     2881 cgatcattcc aaagtgaaaa aagttttcgg ccggcgtgcg atcactccgc tttccgaagg
     2941 gctcgcgcgc atggcggaat gggcgaaacg cgccggcgtg cgtaaaagca aacgtttcgg
     3001 cgagatcgag atccacgaaa agcttccgcc cgtctggctg gaagaataaa ccctgcgtcc
     3061 cggctaccct gcggaaaaat ggtcccctac aaaagcacac ctttacatcc tgttgaaacc
     3121 gccggcggaa cgatcgattc cttcatttcg ggaacgatca acgccgattc tccgaccgta
     3181 aagtccttcg gcgacgaatg gaaccggttc tcctcattca cggaagaaga gatacagcgt
     3241 gcaggcgaac aatacttcga catcgtgacg gaagaaatgg tgaacaaaga caccgtcgcg
     3301 ctcgatatcg gctgcggaag cgggcgctgg tcgaaattcc tgtcgccgcg cgtgaaattc
     3361 attgaagccg tcgatcccgg cgaagccgtt cacgcagccg ttacactgac gaaaaattgc
     3421 ggaaacatcc gcatcacgca ggccggctac agaaatatcc cgttcgaaaa aggatcgttc
     3481 ggtttcgtgt tcagcctcgg cgtcgtgcac caccttcccg atacagcggg cgctgtgcag
     3541 gaagccgctt cgctggtaaa aaagaacggc tggctgctgc tgtacattta ttacaatctc
     3601 gataaccgcg gagcgcttta ccgcctgctt ttcggcctgt cgtctgttat gcggagaata
     3661 atttcccgtt ttccgcgcgg actgaaattt tttgtctgtg aattaatcgc gatttttgtt
     3721 tacggtcctt tcgttctctg cgcacgcttc ctgaaacttt tcggcggaca ttcctggaaa
     3781 aaaatcccgc tttcctatta cgccgacaaa ccctggaaag tgatccgcaa tgattcactc
     3841 gaccgtttcg ggacgccgct ggaaaagcgc ttttcccggg aggaaattga aaaaatgctg
     3901 ctggacgccg gcatggacga tatccgtttt tcggagaacg aaccctactg gcacgttgta
     3961 tcgcgcaaaa agtgaaacgg aaaaagatcc tcttcgttgc atcacaccgc cccgggcgcg
     4021 cccccgggca gcgtttccgc tttgaacagt atttcgattt cctgcggcag aacggtttcg
     4081 actgtgagct gtcgtatatc atttccgaga acgacgacaa gctgctgtac cgcaaaggcc
     4141 attacctcga aaaattttcc attcaccgcc gttcgctgaa aaagagaaag aacgacgtgc
     4201 tccgcgctgg tgagttcgac atcatcttca ttttccgcga agccatcgtc acgggctccg
     4261 tgaagttcga aaagctgttc cggaaatcag gtgcgaagat cgtcttcgat ttcgacgacg
     4321 ccatctggca cctcgacgtt tccgaagcca accgcatgtt cgggttcctg aaacgtccgc
     4381 agaaaaccgg cgagatcatc ggcttgtcgg acctcgtgtt cgcgggcaac aattacctcg
     4441 cggaatacgc gcggaagttc agcacgaatg tcgcgatcgt tcccaccacc atcgacacgg
     4501 aggaatacaa agcggaaatt cccgccggga agaacgggaa gatcaccatc ggctggagcg
     4561 gcagtcttac caccataaag catttcgagt tcgccgttcc tttcctgcgc gagctgaaga
     4621 aaaagcacgg ggataaaatt tccgtgaaag tgatcggcga cggaacgtat gtgaacgaag
     4681 agctgggcgt gaaaggcatt ccctggaaaa aggaggatga ggtgaaagag ctttccacgt
     4741 tcgacatcgg catcatgcct ttgcccgacg acgaatgggc gaagggaaaa tgcggactga
     4801 aaggattgca gtacatggcg ctcggcattc ccacgatcat gtcgccggta ggtgtgaaca
     4861 ccgagatcat cacggaaggc gtgaatggtt tcctcgccac atcgacggaa gaatgggtgc
     4921 ggaagatcga atcgcttatc acttcggaag agctgcgcag gaaaatggga agcgaagcga
     4981 ggagaaccgt cgaggaaaaa tattcggtca gtgcgtggaa ggaaaaatac ctggagctgc
     5041 tgcgctcgct gtaacggctt tgtttcaacg gtaacccgct tcgtccttcc gcttattctt
     5101 ttttactgca acacgaaata cttacctgca aaatccaaaa cgcggcgcgg tcgtccgtca
     5161 aacccggaac agctgcctgt tttatgttaa atttgcgctt cacatttcat cttatggacg
     5221 caacagctac actgaaaaat accgcgctta cggcaaagca cgtcgcgctc ggcgccaaaa
     5281 tggttccttt cgcgggatac aacatgcctg tttcctatac gggacttaat gaagagcatc
     5341 ttaccgttcg caacgcggtc ggcgtgttcg acgtttcgca catgggcgaa ttcatcctga
     5401 aaggcgaaaa agcgctcgat cttatccagg ccgtcacgac aaacgatgcg tcgcagctgt
     5461 acgacggacg tgtgcagtat tcctgtcttc ccaatggaaa aggcggcatc gtcgacgacc
     5521 tgctcgtgta ccgcatcgat gaaaaaacgt acatgctcgt ggtgaacgcg tccaacatcg
     5581 ataaagactg gaactggatc tcccggcaca attcctacgg cgtcgagatg aaaaatattt
     5641 ccgaacgcac atcgctcctt gccgtgcagg gcccgaaagc gcaggccacg ctgcagaagc
     5701 ttaccggcat caacctcgcg gaaattccgt attaccattt caaaaaagga aaattcgcgg
     5761 gcgaagacaa tatcctcatc tccaacaccg gctacaccgg cgcaggcgga ttcgaaattt
     5821 atttcgacaa tgctgttgct gaaaaaatgt gggacgccgt tttcaaagcg ggggaagagt
     5881 tcggcatcaa acccatcggt ctcggcgcgc gcgacacgct ccgtcttgaa atgggattct
     5941 gcctttacgg caacgacatc gacgacacga catcgcccat tgaggcgggc ctgggctgga
     6001 tcacgaaatt caacaaagag ttcgtcgata aagacaaaat gcttgcgctg aaagaaaaag
     6061 gcgtggcgaa aaagctcgta gggtttgaaa tgatcgaccg tggcattccc cgtcacgatt
     6121 acgagatcac cgacgcgaac gggaacgtca tcgggaaagt gacgtcgggc acgcaatcac
     6181 cttcactgca gaaaggcatt ggcatgggct acgtgaaaac ggaattcagc aaaacaggta
     6241 cggaaattta cgtgaaggtg cgcgataaag ccctgaaggc gcaggtggtg aaagtcccgt
     6301 tcctgaaatc ataacgggta acgccgatgt ccacagaaac cgccatccgt atcgacgcct
     6361 gtttttcacc ttacctctac ccggtgtaca gggacgaagg aagcatcgtg gtcatcatcg
     6421 acattcttcg cgcgacatcc gccatctgca ccgcttttca ttacggcgct gaaaaaataa
     6481 ttcccgtcgc cacggtggaa gaagcccgtg cgctgaagga aaaaggattt ctcgccgggg
     6541 ccgagcgcaa cgccatacgc gtggaaggtt ttgatttcgg gaattcgcct ttcgattata
     6601 tgggtgaaga catcgcgggg aaaaccatcg tgctgacgac gacgaacgga acgcaggcta
     6661 tcgaagcgtc gaagtacgcg cacaaagtgg tgatcggcgc gttcacgaac atcagcgcgt
     6721 tatgcgactg gctcgtgaag caggaccgca acgtgctcct cctctgctcc gggtggaaaa
     6781 acaaattcaa cctcgaagat tcgctttttg ccggcgcgat gaccgcggag ctcgtgaaaa
     6841 gaaatcccgc ctacaagctc ggcgacgcct gcctcgcgct cgaatatctt taccagatgg
     6901 cgaaaaagaa tcccgtgaaa ttcctcggcc gcgcttcaca caaggaacgt ctctcaaagc
     6961 tgaacctgaa agacgacatc cgctattgcc tgcagacgga cctcacgacc gtcattcctg
     7021 ttcttgaaga cggggcgctg gtgaaaatgt gaagacttcg cttttttgat ctccgcgtaa
     7081 cattttccat cttttcaatc cttaactttg ttttatgcgc aaagttttcc ggaactccgt
     7141 cctgcttttc gtcctgctgt ccattgtcgt cttttcattg ccgcagcagc gggcggaatc
     7201 atggggattt tacgcgcaca aacgcatcaa ccgcatggcg gtgttcacgc tcccgccgga
     7261 gatgttcggc ttctataaaa agcacatcga gtacatcacc gaacacgccg tcgatcccga
     7321 caagcgccgt tattccgttg cggaagaagc gccgcggcat tacatcgaca tcgatcatta
     7381 cggaccgaac tgcttcgaca gcgttccccg gctctggaaa aaagccgtcg ccaaatacac
     7441 ggaggacacc ctcaacgctt acggcatcgt gccctggtgg atcgacgtga tgacctaccg
     7501 cctgaccaag gcattcaagg acggcgacgt ggaccgcata ctccggcttt cagccgagct
     7561 gggtcattac gtgggcgacg cacatgtgcc gctgcatacc acagaaaact acaacgggca
     7621 gatgacgaac caggtgggca ttcacggttt ctgggaatcg tcggtgccgg aaatgttcgg
     7681 cgaccagtac gattatttcg tgggccgggc cgaatacatc gactcaccga tcaatgccgc
     7741 gtgggcaatc gtgaaggtca gccacagcga agtggattcc gtcctcagcc tcgaggcaca
     7801 gctcaacgcc accaccccgc ccgaccggaa atacgcattc gtgaaccggg gcaatacgac
     7861 ggttaaaacc tacagcgagg aatacacggc cagctacagc cggatgctca acggcatggt
     7921 ggaacggcgc atgcgggcgg ccatccacat ggtgggaagc ctctggtata ccgcctgggt
     7981 gaacgccggc cagccggatc tttcaaagct cgaggacaag gacgtcagcg attccgccaa
     8041 aaaggcccag gagcaggaag aatacctctg gaagaatggc aagctcaaag taaagggcca
     8101 cgaggactga cccggttttc ccgccaaagt tgacttctgt taattttttt gaagacgtgt
     8161 aaaacggaca tatccggcgg ttacctttgc tctgctccag cgaaacaatg aaaatttctt
     8221 tgcggaatta ttttctgatc gtggtgcctg tattggcaat ctgctgcatt ttttcgggac
     8281 aaggtattct ccgcttcatc gaaaaaagcc agaccatttc ctccaacgct attctctcca
     8341 cttcttacga cgacgactca atatccgcca ccgcggcgga cgatgacggg gacgatgtct
     8401 ccgaaacgct gttccggctg ctgcccgttt ctcttgttgc cgtttttgtt tatctcttcc
     8461 tgcattcggt tccgcaggct tctttctctt tgctgcctca cggggcaccg aagcaggttt
     8521 cgcccgtctt tctcggcgtg ttcaggatct gacctggagc gacgtatcat ccttacgcct
     8581 cttcaccaag aaatcctgta acacaactat gattcgtctt cctgttccga agacgctgct
     8641 tcctgtgatg gccctggtgg tcctgtcatt cggaaaaaca gctgcaacgg aagtactgaa
     8701 aacagacaca gttccgctgg acgttaacac agcggagcaa cgcctcgtca gcaaaagcct
     8761 gatgctgctt gcggcacgat acgacgtgga catcgccaaa tc
//
LOCUS       DASTSF010000497         2310 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1878237,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000497 DASTSF010000000
VERSION     DASTSF010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2310
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1878237"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1035)
                     /locus_tag="VFU15_14305"
     CDS             complement(<1..1035)
                     /locus_tag="VFU15_14305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719012.1"
                     /translation="MTTLYYIPDVQLPESQDFNELMSEGRSFIQSHIGNEWTNLNASD
                     PGVTILEQLCYALTELGYCIDFPMRDILTRKDGELETKDQFYFPEDILTTSPVTPDDY
                     RKYLIDGVSNTGNAVVVQADGAYPVFLRNCMVYLMPVKTVSGVLAEAFCKSAFYYLNR
                     NRYLATVFSQPEILADNAYYLQGTITISDGSGKSKILALVEESIRNYIFPTVIPASYA
                     TLTADSVATNDIFNGPHLENGWIPSSSLGVNRTQLWASELYSVIKSVSGVTEVNALGF
                     VPVSTSQATGSTSSESTDQITSSLSELIVIKVKDSGFIIVESPQPSKQTTPDPLLVAA
                     ETIEAEFDLNI"
     gene            complement(1032..>2310)
                     /locus_tag="VFU15_14310"
     CDS             complement(1032..>2310)
                     /locus_tag="VFU15_14310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719013.1"
                     /translation="DLSNTSVDFPLQLFYQTPFANYAAYDNSASETASATIYNSIAGP
                     STGAEGVLLFPSFAYSGALYIELSALAAGNTINLYFEQSRGNAVSTSTPVFSYFYLNA
                     SGWNELLPISDGTSGFNCSGILQLNIPADIVTGSPVVPGSNCWLLITTNADLPSFAQT
                     VLLSANGVLLQRTGDTYQSDVTAPTLAAGSITKPQTAIPSISTITQPFDSFGGTQAED
                     ETTMNLRVSNRLKTKDRAVTYGDFYTLISQSFPGVYYSSSKYDGESGTVNVYLVKKSS
                     SASVANAFSPLVSDCTETQVEAFLAQRVSLFTSLIVSNYAIQPLSVTANVSIEQGYAW
                     QYVLGAITTAIDLFLSPWIECDATQADIGKAILPGEISSLLLSIGGVESVTGVTFSSV
                     SGSAGPGGSVVPLAGSILVTTRKHTINLVTTNG"
BASE COUNT          501 a          561 c          703 g          545 t
ORIGIN      
        1 gatattcagg tcgaattccg cttctatcgt ctcggctgcg accagcagcg gatccggtgt
       61 cgtctgcttc gaaggttgcg gagattctac gatgatgaat ccggaatcct tcacctttat
      121 gacgatcagc tcggaaagag acgaggtgat ctggtcggta ctttcagaag atgtgctgcc
      181 cgtggcctgc gaagtggaaa ccggaacaaa gccgagggca ttcacttccg ttacgccgct
      241 gacggatttg atgacggaat aaagttccga agcccacaat tgagtgcgat tgacgccgag
      301 cgaagatgaa ggaatccacc cgttctcaag atgcggtccg ttgaagatgt cgttcgtagc
      361 gacgctgtcg gcagtgagcg ttgcgtagct cgcgggtatg acggttggaa aaatgtaatt
      421 gcggatgctt tcttccacca gcgcgagtat tttggatttt ccgctcccgt cgctgatggt
      481 gatcgtgccc tgcaggtaat aggcgttgtc cgcgagtatt tccggttggg agaaaacagt
      541 ggcgaggtac ctgttcctgt tcaggtaata gaatgcgctt ttgcagaacg cctcggcaag
      601 aacaccggaa acagtcttta ccggcatgag ataaaccatg cagttgcgca ggaaaacagg
      661 atacgctccg tctgcctgca cgaccacggc attgccagtg ttactgaccc cgtcgatgag
      721 gtatttcctg taatcgtccg gtgtgacagg gctggtcgtg agaatgtctt ccgggaaata
      781 gaactggtct ttcgtttcca gctccccgtc tttgcgtgta aggatgtcgc gcatcgggaa
      841 atcgatgcaa tatccgagtt ccgtgagcgc atagcagagc tgctcgagta tcgtcacacc
      901 gggatcgctt gcgttcaggt ttgtccactc attcccgatg tgcgactgga tgaaggaacg
      961 tccttcgctc atcagctcgt tgaagtcctg gctttccgga agctgtacat cgggtatgta
     1021 atacagtgtt gtcatccgtt cgttgtaacc aggttgatgg tatgctttcg tgtcgtgacc
     1081 aggatcgaac ccgcgagcgg aacgacggat ccgccgggac ctgcgcttcc gctcaccgag
     1141 gagaatgtta cgcctgtcac agattcaacg ccgccgatgc tgagcaggag tgaagagatc
     1201 tcgccgggaa ggatcgcttt cccgatatcg gcctgcgttg cgtcgcattc gatccacggt
     1261 gaaaggaaaa gatcgatggc cgtcgtgatc gcgcccagca cgtattgcca tgcgtagcct
     1321 tgttcaatgg aaacgttcgc cgttacggaa agcggctgga tcgcataatt cgaaacgatg
     1381 agcgaagtga agagcgacac gcgttgtgcg aggaacgcct ccacctgtgt ttctgtgcag
     1441 tcgctgacca gcggggagaa tgcattcgcc acggaagcgc tgctgctttt cttcacgagg
     1501 taaacgttga ccgtgccgct ttcaccgtcg tatttcgaag aggaatagta aacaccgggg
     1561 aatgactggc tgatgagtgt atagaaatct ccgtacgtga cagcgcggtc ttttgtcttc
     1621 aggcggttgc ttacacgcag gttcatcgtc gtttcgtctt ctgcctgtgt tccgccgaat
     1681 gaatcgaacg gttgcgtgat ggtggatatc gaagggatgg cggtctgcgg tttggtgatg
     1741 cttcccgctg cgagtgtggg agccgtgacg tccgactggt acgtatcgcc tgtgcgctgc
     1801 agcagcacgc cgttggcaga aagcaggacc gtctgtgcga aggaaggaag atcggcattc
     1861 gtcgtgatga gcagccagca gttgcttccc ggcaccacgg gtgaacctgt gacgatatcg
     1921 gccgggatgt tcagctggag gatgccgctg caattgaagc cgctcgttcc gtcgctgatc
     1981 ggcagcagct cattccaccc ggatgcgttg agatagaaat acgagaagac gggtgtcgag
     2041 gtggatactg cgttgccgcg cgattgctcg aaatataagt tgatcgtgtt gccggctgcg
     2101 agcgcggaaa gctcgatgta caaagcgccg ctgtacgcga aggatggaaa aagaagaacg
     2161 ccttcggcac ctgttgatgg acctgcaatg gaattgtaaa tggtggcgga ggcggtttca
     2221 ctcgcagaat tgtcgtacgc tgcataattt gcgaacgggg tctggtaaaa aagctgcagc
     2281 ggaaaatcga cggacgtatt gctgagatcg
//
LOCUS       DASTSF010000498         4510 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1280228,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000498 DASTSF010000000
VERSION     DASTSF010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4510
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1280228"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..706)
                     /locus_tag="VFU15_14315"
     CDS             complement(<1..706)
                     /locus_tag="VFU15_14315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004584418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
                     /protein_id="HEU4719014.1"
                     /translation="MFDSLSEKLERAFKVLRGEGKITEINVAETVKDIRKALLDADVS
                     YQVAKKFTDDLKTKALGQNVLTAVSPGQLLVKITHDELATLMGGSMEEIKLGGSPTII
                     LMAGLQGSGKTTHSGKLAYWLKNKKGKNPLLVACDVYRPAAVDQLKVLGEQIGVAVYA
                     EPDVKDPVGIAKRAVAKAKQDGNSVVIIDTAGRLAIDEMMMNEIAAVKKAVDPDEILF
                     VVDSMTGQDAVNTAKAF"
     gene            719..1204
                     /locus_tag="VFU15_14320"
     CDS             719..1204
                     /locus_tag="VFU15_14320"
                     /inference="COORDINATES: protein motif:HMM:NF013633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEU4719015.1"
                     /translation="MENEDTEFGSLVFLFTARVLHFRRPSSKSPVVPIFTGTMAENLR
                     IDKWLWSVRLFKTRSLATEACKGGRIKLNGDAAKPAKELKAGDEISFKSGPITRTVRV
                     KDFPPSRIAAKFVASFYDDLTPEEEYERLRLMKEVGPSVFYTGKGRPTKRDRRKLGDL
                     F"
     gene            1241..1510
                     /locus_tag="VFU15_14325"
     CDS             1241..1510
                     /locus_tag="VFU15_14325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017730300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein"
                     /protein_id="HEU4719016.1"
                     /translation="MAELNFDELKKKLEKNPWPSVYMFKFIVPSDNHRIALVESLFGD
                     DAEVKHQPSSEGKYTSITAREVMLSADAVIEVYKKASTIEGIIAL"
     gene            1658..3874
                     /locus_tag="VFU15_14330"
     CDS             1658..3874
                     /locus_tag="VFU15_14330"
                     /inference="COORDINATES: protein motif:HMM:NF013123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HEU4719017.1"
                     /translation="MKIRFLFLFFLASLVVSAQDQMLSVENAVFGYYQGLYPNRLSQL
                     SWIPGTNTYSWVDKVNNVETLLTATAGDSKSRKSILTLDELNSKVQQLNKGIDAFKMF
                     PAVSWTNDAMFRIATEKMDLIYSVATQSFTTYDYAKDAANFDRSDASGEIAYTTGNNL
                     WVSLIHVDLSMQNNGKVTYENVAVSNDTNRNIVYGQSVHREEFGITKGTFWSPSGMLL
                     AFYRMDQTMVTNYPIYDLTKQPAEVRMIKYPMAGATSHQVTVGVFDVKTQKVIYLKTG
                     EPKDQYLTNIAWSPDNKFVYIAVLNRDQNHLWLNCYNASTGDFVKTLFEETNEKYVHP
                     MHPMVFVPMHPDEFIWQSERVKGNALYLYKTDGTLIRPLIGSGVSRTNVAPLLVTDFY
                     GVDAKGKTAYFQATYFNGIGRMICSVDLEKGNPQSLTQYDGTHAAMFSADKSFFIDDF
                     SSLTVPHIQTLCKASGKSVSVLCTAANPLASYKKCNVRLFTITAADNTTPLWCRMILP
                     ADFDSTKKYRSLTYVYNGPNVQLVTNSWLGGSDLFLYYMAQQGFVVFTVDGRGSDGRG
                     IDFEQATFRHLGTQEIADQQKGAEYLKKLPYVDASRMAVYGWSFGGFMTTGLMTRTPD
                     LFKCGVAGGAVIDWSYYEVMYTERYMDTPQQNPDGYKEANLLNYNQNLKGKLLMIHGT
                     SDDVVVWQHTLLYTQSCVSKGVQTDYYVYPGHPHNVRGKDRVHLLTKVSQYIMANT"
     gene            complement(4092..>4510)
                     /locus_tag="VFU15_14335"
     CDS             complement(4092..>4510)
                     /locus_tag="VFU15_14335"
                     /inference="COORDINATES: protein motif:HMM:NF025749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4421 family protein"
                     /protein_id="HEU4719018.1"
                     /translation="SRFFYERYGDLNYFRVQGISIGPGYSFNLVLWKTLYFNGTATSG
                     FDFQHRSYGNYSTGTIDKFWKVGFAGDLRFAIGLNGKHMFASATYRVDYNSYVTNGMR
                     IYPFYQSIDFNIGYRFPFKERKWVKKMKANKWYQML"
BASE COUNT         1135 a         1271 c         1125 g          979 t
ORIGIN      
        1 tgaaggcttt cgccgtattc accgcatcct gtcccgtcat ggaatcgacg acgaaaagaa
       61 tttcatccgg atccaccgct ttcttcactg cggcgatctc gttcatcatc atttcatcaa
      121 tggcgaggcg gccggcggta tcgatgatca ccacggaatt cccgtcctgc ttcgctttcg
      181 cgaccgcgcg cttcgcaatg ccgacaggat ctttcacatc gggttccgca tacacagcca
      241 cgccgatctg ctcaccgagc actttcaact ggtcgacagc agcgggacgg tacacgtcgc
      301 aggcaacgag caatggattt tttccttttt tatttttcag ccagtaggcg agcttccccg
      361 aatgcgttgt tttaccggag ccctgcagac cggccatgag aatgatcgtg gggcttccgc
      421 cgagctttat ctcctccatc gatccgccca tgagcgttgc gagctcatcg tgcgtgatct
      481 tcacgagcag ctgtcccggc gaaacggcag tgagcacgtt ctggccgagc gctttcgtct
      541 tgaggtcgtc ggtgaatttc ttcgctacct ggtagctgac gtctgcatcg agcagcgcct
      601 tgcggatgtc ttttaccgtt tccgcaacgt tgatctcggt gatcttgcct tcaccgcgga
      661 gcaccttgaa cgcgcgctcc agcttttcac ttaaactgtc gaacatgtga ttggaaaaat
      721 ggagaacgaa gatacggaat tcggctctct tgttttcctg ttcacggcgc gggtcctgca
      781 tttcaggcgc ccgtcttcaa aatcccccgt cgttcctatc tttacaggaa caatggccga
      841 aaatctccgt atcgacaagt ggctgtggag cgtacgtctt ttcaaaacac gttctctcgc
      901 tacggaagcc tgcaaaggcg gcagaataaa gctcaacggc gatgcggcca agcccgccaa
      961 agagctgaag gccggcgatg aaatttcctt caaaagcggc ccgatcaccc gaaccgttcg
     1021 tgtaaaagat tttccacctt cacgcatagc ggctaaattc gtcgcatcgt tttacgacga
     1081 cctgacgccg gaagaagaat acgaacggct tcgcctgatg aaagaggtag gcccttccgt
     1141 tttctacacg ggaaaagggc gccccacgaa acgcgaccgg cgcaagctcg gcgatctttt
     1201 ctgaacgttg aaactgaatc cgcatcctga acttattttt atggctgaac tgaactttga
     1261 cgagctgaaa aagaaactgg aaaagaatcc ctggcccagc gtgtacatgt tcaagttcat
     1321 cgttccgtcc gacaaccacc ggatcgcgct cgtggaaagt cttttcggcg acgacgcaga
     1381 agtgaagcac cagccgtcat ccgaaggaaa atacaccagc atcactgccc gcgaggtcat
     1441 gctttccgcc gacgccgtga tcgaagttta taaaaaagca tcgacgatag agggaattat
     1501 tgcgttgtga cgaaatgcga gcagtgcaat gagaggaatg aagaacgtat tgcacacatc
     1561 gagagacgaa ctgaaaacaa ttcaaaccac aggcacccga ccacccattc aaatccgaaa
     1621 tccgaaaccg ataaccaata aaacgaccga ccgcaaaatg aaaatccgtt tcctttttct
     1681 cttcttcctt gcctctcttg ttgtttccgc gcaggaccag atgctcagcg tggagaacgc
     1741 cgtattcggc tactaccagg gtttgtaccc gaacaggctt tcacagctca gctggatacc
     1801 gggaacaaac acctattcct gggtcgacaa agtaaacaac gtggaaacgc ttctcaccgc
     1861 cacggccggc gattccaaaa gccggaaaag cattctcacg ctcgacgagc tgaacagcaa
     1921 agtgcagcag ctcaacaaag gcatcgatgc gttcaagatg ttcccggctg tgagctggac
     1981 gaacgacgcg atgttccgca tcgccacgga gaaaatggac cttatttatt ccgtcgccac
     2041 gcaatcgttc accacctatg attatgcgaa agacgccgcc aacttcgacc gttccgatgc
     2101 ttccggcgag atcgcgtaca cgacgggaaa caacctgtgg gtttcgctca tccacgttga
     2161 cctgagcatg cagaacaacg gcaaggtcac gtatgaaaat gtcgctgttt ccaacgacac
     2221 gaaccggaac atcgtttacg gacaatccgt tcaccgcgaa gagttcggca ttacgaaagg
     2281 aacgttctgg tctccgtcgg gaatgctgct cgcgttctac cgcatggacc agaccatggt
     2341 gacgaattat cccatttacg atctcaccaa acagccggcg gaggtgcgca tgatcaaata
     2401 cccgatggcg ggcgccacgt cgcaccaggt aaccgtaggc gtgttcgacg taaaaacgca
     2461 aaaggttatt taccttaaaa caggggaacc gaaagatcag tacctgacca acatcgcgtg
     2521 gagcccggac aacaaattcg tttacatcgc tgtgctcaac cgcgaccaga accatttgtg
     2581 gctgaactgc tacaacgcat ccacgggcga tttcgtcaaa actcttttcg aagagacgaa
     2641 cgaaaaatac gttcatccca tgcacccgat ggtgttcgtt cccatgcatc ccgatgaatt
     2701 catctggcag agcgaacgcg ttaaaggaaa tgcgctttac ctctataaaa cggacggcac
     2761 gctcatccgc ccgcttatcg gatcaggtgt atcgaggaca aacgtcgccc cgctgctcgt
     2821 cactgatttt tacggcgtcg atgcgaaagg caaaaccgct tatttccagg cgacgtactt
     2881 caacggcatc ggccgcatga tctgcagcgt cgatcttgaa aagggaaatc cgcaatcact
     2941 gacgcaatac gacgggacgc acgccgccat gttctctgcg gacaaatcat ttttcattga
     3001 cgatttttcc tcgctcaccg ttccgcacat ccagacgctt tgcaaggctt ccgggaaatc
     3061 cgtgagcgtc ctgtgcaccg ccgcgaaccc gctcgcttcg tacaaaaaat gcaacgtgcg
     3121 cctgttcacg atcaccgctg cggataacac cactcctctc tggtgccgca tgatccttcc
     3181 cgcggatttc gactccacga aaaaataccg ctcgctgacg tatgtataca acggacccaa
     3241 cgtgcagctc gtcacgaact cctggctcgg cggttcggat cttttcctgt attacatggc
     3301 gcagcaggga tttgtcgtgt tcaccgtcga cggaagaggt tcggacggac gcggcatcga
     3361 tttcgagcag gcgacgttcc gtcacctcgg cacgcaggag atcgccgacc agcagaaagg
     3421 cgccgaatac ctgaaaaagc tcccgtatgt cgacgcatca cgcatggcgg tgtacggatg
     3481 gagcttcggc ggattcatga ccaccggact gatgacgcgc acgcccgatc ttttcaaatg
     3541 cggtgtcgcc ggaggcgcgg tgatcgactg gagctactac gaagtgatgt acacggaacg
     3601 ttacatggac acgccgcagc agaacccgga cggttacaaa gaagcgaacc tgctcaacta
     3661 caaccagaac ctgaaaggga aactgctgat gatccacggc acgtcggatg acgtcgtcgt
     3721 gtggcagcac acgctcctgt acacgcagtc gtgcgtgagc aaaggcgtgc agacggatta
     3781 ctatgtctat cccggccatc cccacaacgt gcgcggaaaa gaccgcgtgc acctgctgac
     3841 gaaagtttca caatacatta tggcgaatac gtgagaaaaa tgaggattgc gtcccgcgtt
     3901 ctccgtgatt ctccgtgaaa cgacctccgt ggttctccgt gttaaaatac cgcatgtggc
     3961 gactggtatt gtaatcacca caaagtgctc aaagttttgc acgaagaaca caaagctgtg
     4021 ttaaaataat ttccgcaaag acgtgacaga aattttctgc gaattacacg cgtcaggctg
     4081 ccgcgggaag atcagagcat ctggtaccac ttgttcgctt tcatcttctt cacccacttg
     4141 cgttccttga acgggaaacg gtagccgatg ttgaaatcga tcgactggta gaagggataa
     4201 atgcgcatgc cgttcgtgac gtaggaattg taatcgacgc gatacgtggc agaagcgaac
     4261 atgtgcttcc cgttgagccc gatcgcaaaa cgaagatcgc cggcgaaacc cactttccag
     4321 aatttatcga tcgtgcccgt ggaataattc ccgtagctgc ggtgctggaa atcgaaaccg
     4381 ctggtcgccg taccgttgaa ataaagcgtt ttccagagca cgagattgaa ggaataaccc
     4441 ggcccgatgg aaattccctg cacacgaaaa taattcaggt cgccgtaccg ctcatagaaa
     4501 aaccgtgacg
//
LOCUS       DASTSF010000499         2474 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1883667,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000499 DASTSF010000000
VERSION     DASTSF010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1883667"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(358..1308)
                     /locus_tag="VFU15_14340"
     CDS             complement(358..1308)
                     /locus_tag="VFU15_14340"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU4719019.1"
                     /translation="MSNALQQIFSRVHKLTGVKRRGEESLESSILRGLEILEEKREAK
                     IAGSAAGLDRIQEILGVIMSLANLDYNHKAPVSDALDEIDALAGGINMLGEELKSSTV
                     SLHEKETLLKEIHHRVKNNLQVISSLLNLQADRIEDPLAREKFLVCRERIRTMSMVHE
                     KLYESGNLSSIDFPEYIESLARTLNNSYNTDKTLVKLHTDVGNLDPEDRRFKIETAIP
                     CGLILNELLSNAYKYAFPEQRKGNVRVLFDVDRGAKAGHYRIRVEDDGVGLPEKFLPE
                     KAETLGMQLVWILAEQLEGKLDVESGKKGTAITLTFNGNA"
     gene            complement(1312..1695)
                     /locus_tag="VFU15_14345"
     CDS             complement(1312..1695)
                     /locus_tag="VFU15_14345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS/SEC14 domain-containing protein"
                     /protein_id="HEU4719020.1"
                     /translation="MIETRVFTTSLNKEQGYLLTHVKPGSEIGVNDARENTEAVISLS
                     GGRNFPIVVDLRKIKSISKEARDHFSMRGRKPHVTAIAMVVSSPLSRIIGNFFLGLNK
                     PTVPTRMFSSEADGIKWLSKEGLLD"
     gene            complement(1720..1792)
                     /locus_tag="VFU15_14350"
     tRNA            complement(1720..1792)
                     /locus_tag="VFU15_14350"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1757..1759),aa:Gly,seq:ccc)
     gene            complement(1893..>2474)
                     /locus_tag="VFU15_14355"
     CDS             complement(1893..>2474)
                     /locus_tag="VFU15_14355"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU4719021.1"
                     /translation="EDALNKFRSSGYDLVMTDVMMPKKDGFELVNEIRKESPRVPVIF
                     ITARNRREDMIKGYQKGAMDYITKPLDSELLIYKIKALLGGARRNEHVPELFLFGRWK
                     FETAGRVLHDGKENFRLSPREAELLLALLGEKNSVVKKSVILKKIWGDDSYYNARSMD
                     VYVTRLRGRFRNDPAVVIESVYGTGIKISVGEL"
BASE COUNT          546 a          684 c          601 g          643 t
ORIGIN      
        1 aggacatctg ggaaaagccg ctgccggatg cgatcaaata cctggaagct gcgcacaaga
       61 tcgatcccaa agatgtggat gcctgttacg cgctccagaa cgcttacggc ctccaggggg
      121 ataatgacaa ttacctccgc atgaaagatg agatcaagaa gctgaaagcg ggggaataat
      181 gacccgaaga tgcgacagaa aacaattaaa gtacgagccc cgcgagagtg gggctttttt
      241 tattcattca tgcacaaaca acttgttttc aatactatac tatttaacat aatataaatt
      301 atgtaccaaa aacaaataat taccaggtcg ttataaatcc tgcccggacg tccgggttta
      361 agcattgccg ttgaacgtta gcgtgatggc cgttcccttt tttccggact ccacatccag
      421 ctttccttcc agttgttcgg ccaggatcca gaccaattgc atcccgagcg tctcggcttt
      481 ctccggcagg aatttttccg gcagcccgac cccgtcatct tccacgcgga tccggtaatg
      541 cccggctttg gcaccccggt ccacatcgaa caatacgcgc acgttgcctt ttcgctgttc
      601 cgggaatgca tatttatagg cgttcgacaa aagctcgttc aggatcagtc cgcatggtat
      661 cgcggtttca attttgaacc tgcggtcctc cggatccagg ttgccaacgt cggtatgcag
      721 cttgaccagg gttttgtccg tattgtatga attgttcagt gtacgggcca gtgattcgat
      781 atattcggga aaatcgatgc tggaaaggtt cccggattcg tacagctttt catgcaccat
      841 tgacatggta cggatacgtt cccggcagac aagaaacttt tcccgggcca gcggatcctc
      901 gatcctgtcc gcctgcaggt tcaacaggct tgatatgacc tggagattat tcttgacccg
      961 gtgatgaatt tctttcagca gcgtttcttt ttcgtgcagg gaaaccgtgg agcttttgag
     1021 ctcttcaccc agcatgttga ttccgcccgc cagcgcatcg atctcatcca gcgcatcgga
     1081 aaccggcgcc ttatgattgt aatccaggtt cgcaagcgac atgatgacgc ccagtatttc
     1141 ctggatacga tccagcccgg ccgcactgcc tgcaattttt gcttcccgtt tttcttccag
     1201 gatttcaagt ccccgcagga tgctgctttc gagcgattcc tccccgcgcc gttttacgcc
     1261 ggtaagttta tgtacgcgtg aaaatatctg ctgcagggcg ttgctcatga atcagtccag
     1321 caggccctcc ttgctcagcc atttgatgcc gtctgcttcg gagctgaaca tccttgtcgg
     1381 aactgtgggt ttgttcagcc cgagaaaaaa attaccgata atccggctca gcggcgagct
     1441 taccaccata gcgatcgccg taacgtgcgg tttccgtccg cgcatggaaa aatggtcgcg
     1501 cgcctctttc gagatggatt ttattttgcg caggtcgaca acgatgggaa aattccgccc
     1561 cccgctcagg ctgatcacgg cttcggtgtt ctccctggcg tcattgacgc cgatctccga
     1621 acccggtttt acgtgcgtga gcaggtagcc ctgctcttta ttcagtgatg tggtaaaaac
     1681 gcgtgtttcg atcataaaca aaaatcactg aaaagtgtga gcggaaaacg gggttcgaac
     1741 ccgcgacctt cagcttggga agctgacgct ctacctggtg agctatttcc gcttgcctgc
     1801 ctggtttcac agaaagcaaa aatacgattt ggcggaaagc ttcgacaacc ggacccgaat
     1861 ctgtgaaaaa gaagtgcgta acgggtgttc cgttacaatt ccccgaccga aatctttatc
     1921 cccgtcccgt aaacagattc gatcacaact gccggatcgt tgcggaagcg cccgcgcagg
     1981 cgcgtcacgt atacgtccat gctgcgcgcg ttgtaatagc tgtcgtcgcc ccagatcttt
     2041 ttcaggatga ccgatttttt caccacgctg ttcttctcgc ccaggagcgc gagcagcagt
     2101 tcggcttccc tcggggaaag gcggaaattc tctttcccgt cgtgcaggac gcgtccggct
     2161 gtttcaaatt tccatcttcc gaacaggaat aattccggca catgctcatt ccttcgtgct
     2221 ccgccgagca gcgctttgat cttgtatata agcagctctg aatccagcgg cttcgtgatg
     2281 taatccatcg cacctttctg gtaacctttg atcatgtcct ccctccggtt tctcgcggta
     2341 ataaagatca ccggcacccg gggcgattct ttccggatct cgttcaccag ctcgaatcca
     2401 tcctttttcg gcatcatcac gtccgtcatg acaaggtcgt acccgctgct gcggaattta
     2461 ttcagagcgt cttc
//
LOCUS       DASTSF010000500         3585 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_985173,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000500 DASTSF010000000
VERSION     DASTSF010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3585
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_985173"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..669
                     /locus_tag="VFU15_14360"
     CDS             <1..669
                     /locus_tag="VFU15_14360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014472751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HEU4719022.1"
                     /translation="KVLIVLEGRDAAGKDGTIKRITRHLSPRETMVVALGKPSDRDNS
                     SWYFQRYARYLPAGGEIVIFNRSWYNRAGVEKVMGFCSEKQYKSFFTEVGSFEKMLSD
                     AGIIVLKYYLDISRKEQRKRLDDRKRDPLKQWKISPIDQKAQDMWDEYSEARNKMLLK
                     TNFRHSPWYIANAEDKNEVHIALISHLLGLVSYRNKSRKVTGKNFGLVHPATPRNIKT
                     LLHH"
     gene            694..1728
                     /locus_tag="VFU15_14365"
     CDS             694..1728
                     /locus_tag="VFU15_14365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008929853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HEU4719023.1"
                     /translation="MSETYLILFSGIALVMVYDFTNGFHDASDMVATAIAASSVSPKV
                     AISIVTLFTFAGPFLGGMAVADTIGEFVNVHGEAQHAAQSVVLAALMVAITFNLLTWK
                     FGLPSSSSTSLTSGLIGAGLFAFGNASIHWGMTELWKGEIAGFMKIVTGLIFSPLAGF
                     VVGFFLIRLMRKLMSRFTVKTKKALIVTQYLSISWLAFSHGTNDAQKGMGMLAMLLYA
                     SGIYSSFHVPVWVILLCASSITAGTLFGGWSIIRTVGYGIYRVKLIHSIADQIGSAFI
                     ILSSSLIGAPVSTTQVVTTTLMGVGAGERPRHVRWNMAKSIATGWLLNIPFCIVTGAA
                     YCFLLEKLFL"
     gene            1736..2362
                     /locus_tag="VFU15_14370"
     CDS             1736..2362
                     /locus_tag="VFU15_14370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719024.1"
                     /translation="MKLFGKLSDFILPKEVDFFGNLEAQCMVTQDVLKKFCGIYLGED
                     IKTSELTQAITNANELRKKNEAELNEVLITPVDKEAISRAYLNLDWIVLSIKHLNAEA
                     GAYGVSSLQDYGVILGLLKEQMTRMTSCFLLLKEKKYSEVLGGVNDIILLDDEVISEY
                     ASCMAQLFNHDSVQHILRHRELLSQLKEVSKRVHFCVNSVEDIVFKMN"
     gene            complement(2428..>3585)
                     /locus_tag="VFU15_14375"
     CDS             complement(2428..>3585)
                     /locus_tag="VFU15_14375"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEU4719025.1"
                     /translation="DFAWGVAVDHSGNSFLTGNIESPSATFGTTTLTNVGGGDVFIVK
                     YDPAGNEMWAKSSSGSYYDEGESVGTDAAGNCYVTGYFRSPTCTFGSTVLTNTNPSTT
                     NHTMDLFLAKLDASGNILWAKSAGGLSDEEGKAIRVDDDGSCYLAGSSKSNPSAFGSF
                     TLTGAGMFLAKFDSGGNVVWAEGSGGNDLGVGTAICFGPSGKVYSCGYFKAATATFGT
                     HTISNVNSSTYDIFIVETDTSGNYLSAASEGDSDNDFAYGVGTDSTGNIYLSGAFRSA
                     AIAFSPLTLTNIHNMYDDFFIAKSGSLTAVAAENAAPAIPAWPNPANGDFMITVPGND
                     ALVTITDAGGELVYSARTAGGNQPLHLGSPGLYFVTVLSEGKSSTQKIIIR"
BASE COUNT          833 a          985 c          952 g          815 t
ORIGIN      
        1 aaagtgctca tcgttcttga aggaagggat gcggcgggaa aagacggcac catcaagcgc
       61 atcacgcggc acctcagtcc aagggaaacc atggtcgttg cgctgggaaa accttccgac
      121 agggataaca gctcatggta tttccagcgt tacgcgcgtt acctcccggc gggcggcgag
      181 atcgtcatct tcaaccgcag ctggtacaac cgtgccggcg tggaaaaagt gatgggcttc
      241 tgctctgaaa aacaatacaa atcctttttc accgaggtgg gctcttttga aaaaatgctg
      301 agcgatgccg ggatcatcgt gctgaagtat tacctcgaca tcagccggaa ggagcagcgc
      361 aaacgtctcg acgacaggaa gcgcgacccg ctcaagcaat ggaagatcag cccgatcgac
      421 cagaaagcgc aggatatgtg ggatgaatat tccgaagcgc ggaacaagat gctcctgaaa
      481 accaatttca ggcattcgcc gtggtacatc gccaatgcgg aagacaagaa cgaggtccac
      541 attgcgctga tcagtcacct gctcggtctc gtcagctacc gcaacaaaag ccgcaaggtg
      601 accgggaaaa atttcggtct cgtgcaccct gcaacgcccc gcaacatcaa aacgcttctt
      661 caccactgag caggacgagc agcttacccg atcttgagcg agacatacct catacttttt
      721 tccggaatcg cgctggtgat ggtctacgat ttcaccaatg gcttccatga tgcatccgac
      781 atggtggcca cagcgatcgc cgcttcttcg gtgagcccga aagtcgccat ctccatcgta
      841 acgctgttca cgttcgccgg accttttctc ggcggcatgg cggtcgccga tactatcggg
      901 gaattcgtga acgtccacgg tgaagcgcag cacgcggcgc agagtgtcgt gctcgccgcg
      961 ctgatggtgg ccatcacgtt caaccttctc acgtggaaat tcggcctgcc gagctcctcc
     1021 tccacttcgc tcacgagcgg gctcatcggc gcgggacttt tcgcgttcgg caatgccagc
     1081 attcactggg gaatgacgga gctctggaaa ggagagatcg cgggcttcat gaaaatagta
     1141 accgggctta tcttttcacc gctggcagga tttgtcgtcg gatttttcct gatacgattg
     1201 atgcggaagc tcatgagccg gtttacagtg aaaacaaaaa aagcgctgat cgtcacgcaa
     1261 tacctcagca tttcctggct tgcgttcagc catggcacca acgacgcgca gaaaggcatg
     1321 ggcatgctgg ccatgctgct ttacgccagc ggcatttatt cttccttcca cgtcccggta
     1381 tgggtgatcc tgctgtgcgc gtcttcgata acggccggaa ctttgttcgg cggatggagc
     1441 atcatccgca ccgttggcta cggtatttac cgcgtcaagc tcattcattc catcgccgac
     1501 cagatcggtt ccgcgttcat catcctttcc tcttcgctga tcggcgcacc ggtttccacc
     1561 acgcaggttg tcaccaccac gctcatgggc gtcggcgcag gcgaacgtcc gcgtcatgtg
     1621 cggtggaaca tggcgaagtc catcgccacg gggtggctgt tgaatatccc gttctgtatc
     1681 gttaccggtg cagcgtattg tttccttctt gaaaaactgt tcttataaaa acaacatgaa
     1741 gctattcgga aagctgtcgg atttcatcct gccgaaagaa gtggattttt tcggcaacct
     1801 cgaagcgcag tgcatggtca cgcaggatgt gctgaaaaaa ttctgcggga tctaccttgg
     1861 cgaggatata aaaacctcag agctgacaca agccatcacc aatgcgaacg agctccggaa
     1921 aaaaaacgaa gccgagctga atgaagtgct catcaccccg gtcgacaagg aagcgatcag
     1981 ccgcgcgtac ctcaacctcg actggatcgt gctcagcata aagcacctca atgcggaagc
     2041 cggcgcgtac ggcgtttcat ccctgcagga ttacggcgtt attctcgggc tgctgaagga
     2101 gcagatgaca cggatgactt cctgtttcct gctcctgaaa gaaaaaaaat acagcgaggt
     2161 gctgggcggc gtgaacgaca tcatcctgct cgacgatgaa gtgatcagcg aatatgcgtc
     2221 gtgcatggcg cagctgttca accacgactc ggtacagcac attctccgtc acagggagct
     2281 gctttcccag ctcaaagaag tgagcaagcg tgttcacttc tgtgtgaatt cggtcgagga
     2341 tattgtcttc aagatgaact gaaatacggt tcatcggttc atccgggaaa cggcggatga
     2401 aaatggactg ccgggcggaa aacttgctta ccggatgatg atcttctgcg ttgaactctt
     2461 tccttccgaa agaacggtaa caaaataaag tccgggcgaa ccgaggtgca gcggctggtt
     2521 gcctcctgct gttcttgcgg aataaacaag ttctccccct gcgtcggtga tcgttaccag
     2581 tgcgtcgttt ccggggactg tgatcatgaa atccccgttc gctgggttgg gccatgcagg
     2641 aatagcgggc gctgcgtttt ccgctgcgac agccgtcagg ctacccgatt tcgcgatgaa
     2701 aaaatcatca tacatgttgt gaatgttcgt gagcgtcagc ggggaaaatg cgatcgcagc
     2761 gctgcggaaa gctccggaca ggtaaatatt tcctgttgag tcggtgccga cgccgtaagc
     2821 aaaatcattg tcggaatcgc cttcgctggc agcggaaaga taatttcccg acgtatccgt
     2881 ttcgacaatg aaaatatcat acgtcgaaga gttgacattc gaaatcgtat gcgttccgaa
     2941 tgtcgccgtc gccgccttga aatacccgca ggaataaact ttccccgacg gcccgaagca
     3001 gattgcagtt cccacgccaa ggtcattccc tccgcttcct tccgcccaca caacgttgcc
     3061 gccggaatca aatttcgcca ggaacatgcc tgcgcccgtc agcgtgaagc tgccgaacgc
     3121 agaagggttg cttttcgaac ttcccgccag gtaacaggag ccgtcgtcgt caacacgaat
     3181 tgcttttcct tcttcgtcgg aaagtcctcc ggcgcttttc gcccagagaa tatttcccga
     3241 agcgtccagc tttgcaagga aaagatccat tgtatgattc gtcgtactcg ggttggtatt
     3301 ggtaagcacg gtgcttccga atgtgcaggt ggggctcctg aaatagccgg ttacataaca
     3361 gttaccggcg gcatcggtgc ccacgctttc tccttcgtcg taatagcttc cgctggagct
     3421 tttcgcccac atctcgtttc cggcaggatc gtatttcacg ataaaaacat ctccgccgcc
     3481 gacatttgtc agtgtcgttg tcccgaatgt cgcactcgga ctttcgatat taccggtaag
     3541 gaatgaattt ccgctgtgat cgacagcgac tccccaggcg aagtc
//
LOCUS       DASTSF010000501         2970 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1656261,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000501 DASTSF010000000
VERSION     DASTSF010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2970)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2970)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2970
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1656261"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..517
                     /locus_tag="VFU15_14380"
     CDS             <1..517
                     /locus_tag="VFU15_14380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719026.1"
                     /translation="GQLKITGSFDPALMKRFSDNVGNKDSILTLVGEAYRRSDNFLRN
                     GEQDDVATLILAGGWVESLYFALNVYKQKPDHDVAVRIGEQKSTASGVVKVLKDLNDT
                     AKYGQLIRLFSDLNDEYQKVEIKYTFQEPTTDDANHLTNINGNTDVNITPELMTGLMD
                     KVTAIRNYITK"
     gene            514..969
                     /locus_tag="VFU15_14385"
     CDS             514..969
                     /locus_tag="VFU15_14385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719027.1"
                     /translation="MTRNPMKKVYFLLFALIGVFGFSVRSSAQCDSTVKICNSHIIPP
                     FVSDGQTYRALILSGQDAEFSTTFFAGTTYRIAAASGMSEGNIIFSIYSSDPNTGERK
                     LLFTNSQHNNAAYWDFKVNTTVEVVIDAGLNPASGKASGCAVMLIGFKQ"
     gene            1110..>2970
                     /locus_tag="VFU15_14390"
     CDS             1110..>2970
                     /locus_tag="VFU15_14390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU4719028.1"
                     /translation="MSERILKALMQLFAIIAKVDGVTSESRAIVESFLKQQLNQELVD
                     QYLIIFDEFLEQHHQVSKRKDGSAKRTSLNSVKVLRICTQINEELTQKQKVVVLIRLI
                     EFIHSSYEISEQELEFVTTVAETFNIGNSEFVRLRDFVEKDVKEMPDVQEYLLIDGDN
                     TKEYGHSHHIYSETIGNGRVVVVHIPSVGMYACRYFGGAELNLNGQAMILNKVYILTP
                     GSSLRSSKVQPVYYSDIISAFLSSKTGAKITFAANAIEYTFKGGKLGLRGVTVQEESG
                     KLIGIMGGSGAGKSTLLNVLNGNEAPTKGEVLINGINIHTQKDRIEGVIGHVSQDDLL
                     IEELTVFQNLFYNAKLCFGNLGDADISKMVDELLADLGLYEARNLKVGSPLDKKISGG
                     QRKRLNIALELIREPSVLFVDEPTSGLSSRDSENIMDLLKELALKGKLVFVVIHQPSS
                     DIFKMFDKLLILDNGGFPIYYGNPVDAVVYFKKMVNHVNSDESECPTCGNVNPEQIFN
                     IIESKVVDEYGNPTRNRKVSPAEWNQFYREHIEAKQEPVEPAEESPESTFNIPGKIKQ
                     FFVFVTRDVRSKLTNSQYMFINMLEAPVLAFILAYLVRFFNTDVSNTNGYTFRE"
BASE COUNT          755 a          792 c          775 g          648 t
ORIGIN      
        1 cggccagctg aaaattaccg ggagcttcga tcccgcgctg atgaagcgtt tcagcgataa
       61 cgtcggcaac aaagattcca ttcttacgct cgtcggtgaa gcgtaccgcc gcagcgataa
      121 tttcctccgc aacggcgagc aggatgacgt cgccacgctg atcctcgcag gcggctgggt
      181 ggaaagtctc tatttcgcgc tcaatgttta caagcagaaa cccgaccacg atgtggcggt
      241 tcgcatcggt gagcagaaat caaccgcttc cggcgtggtt aaggtactga aggacctgaa
      301 cgatacggcc aaatacggac agctgatccg tctcttctcc gatcttaatg atgaatacca
      361 gaaggtggaa ataaaataca cgttccagga acccacaacc gatgacgcca accacctcac
      421 gaatatcaac gggaacacgg acgtgaacat tacgcccgag ctgatgaccg gccttatgga
      481 taaagtcacc gctatccgca attacatcac gaaatgacca ggaatcctat gaaaaaagta
      541 tattttctcc tcttcgcgct tatcggcgtt ttcggtttct ctgtgagatc gtctgcgcag
      601 tgtgacagca ctgtcaagat ctgcaactcg cacattattc ctccttttgt ttccgacggg
      661 cagacgtatc gcgcgctgat cctctcgggc caggacgcgg aattctcgac caccttcttt
      721 gccggaacaa cttatcgcat tgcagcagct tccgggatgt ccgaaggcaa cattatcttt
      781 tccatttatt ccagcgatcc caatacgggc gagcgcaagc tgcttttcac caacagccag
      841 cacaataatg cggcttactg ggatttcaag gtgaacacaa cagtggaagt ggtcatcgat
      901 gcgggactca atcccgcttc cggaaaggct tccggctgcg ccgtgatgct catcgggttc
      961 aagcagtaac gatcttttaa tgatgcggag gccggccatc agccggcttt cgttttttaa
     1021 ggcttttccc tgcgggcggg gaattaagat taatcaataa ctcccgcgga tttttatatt
     1081 tttacggcca gtccgcacct aaccggaata tgagcgaacg aatactgaaa gccctaatgc
     1141 agcttttcgc gatcatcgcg aaagtcgacg gcgttacatc ggagagcagg gcgatcgttg
     1201 aatcatttct caagcagcag ctcaaccagg agctcgtcga tcagtacctc atcatttttg
     1261 acgaattcct cgagcagcat caccaggttt ccaaacgcaa ggacggatcc gcgaaaagaa
     1321 cttcgcttaa ctccgtaaaa gtgcttcgca tctgcacgca gatcaacgaa gaacttacgc
     1381 agaagcagaa agtcgtcgtg cttatccggc tcatcgagtt catccactcc agctatgaaa
     1441 tatcggagca ggagctcgag ttcgtaacga cggtggccga aacgttcaac atcgggaatt
     1501 cggaattcgt gcgcctgcgc gactttgtcg agaaggacgt gaaggaaatg cccgacgtgc
     1561 aggaatacct gctcatcgac ggcgacaaca cgaaagagta cggccattcc caccatatct
     1621 attcggaaac catcggtaac ggaagagtgg tggtggtgca cattcccagc gtgggaatgt
     1681 atgcgtgccg ctatttcggc ggggcggagc tcaacctgaa cgggcaggcg atgatcctga
     1741 acaaggtgta catcctcaca ccgggttctt cgctgcgcag ctcgaaagtc cagccggttt
     1801 actacagcga tatcatcagc gcgttcctga gcagcaagac aggcgcgaag atcacgtttg
     1861 ccgccaatgc catcgagtat acgttcaaag gtggcaagct gggactgcgg ggtgtgaccg
     1921 tgcaggaaga atcaggcaag ctcatcggca tcatgggcgg ttccggcgca ggaaaatcca
     1981 cgctgctgaa tgtcctcaac gggaacgaag cgccgacgaa aggtgaagtt ctcatcaacg
     2041 gcataaatat tcacacgcag aaagacagga tcgagggcgt gatcggccac gtttcgcagg
     2101 atgatctcct gatcgaagag ctgaccgtgt tccagaatct tttttacaat gcgaagctgt
     2161 gcttcggtaa tctcggtgat gcggacatat cgaaaatggt cgatgagctg ctcgccgatc
     2221 tcggtcttta cgaagcgaga aaccttaagg tcggaagtcc gctcgacaag aaaatttccg
     2281 gtggacagcg caagcggctc aatatcgcgc tcgagctgat ccgcgaaccg tccgtgcttt
     2341 ttgtggacga acctacgtcg ggactttctt cacgcgactc cgagaatatc atggaccttc
     2401 tcaaagagct ggcgctgaaa ggcaagctcg tgttcgtggt cattcaccag ccgtcgtcgg
     2461 atattttcaa gatgttcgac aagctgctca tcctggataa cggcggtttc ccgatctatt
     2521 atggcaaccc ggttgacgcg gtggtttact tcaagaagat ggtgaaccac gtcaacagcg
     2581 acgaaagcga atgcccgacc tgcggtaacg tgaaccccga gcagatcttc aacatcatcg
     2641 aatcgaaggt cgtcgacgaa tacggaaatc ccacacgtaa ccggaaagtg agcccggcag
     2701 agtggaacca gttctaccgc gagcacatcg aggcgaaaca ggaacccgtg gaaccggcag
     2761 aggaaagccc ggagagcacg ttcaacatcc cgggaaaaat aaagcagttc tttgtttttg
     2821 tcacccgcga cgttcgctcg aagctgacga attcacagta catgttcatc aacatgctcg
     2881 aggcgccggt gctggcgttc atccttgcgt acctcgtgcg tttcttcaat acggatgtct
     2941 cgaacacgaa cggctacacg ttccgtgaga
//
LOCUS       DASTSF010000502         2093 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1926102,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000502 DASTSF010000000
VERSION     DASTSF010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2093
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1926102"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..837)
                     /locus_tag="VFU15_14395"
     CDS             complement(<1..837)
                     /locus_tag="VFU15_14395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719029.1"
                     /translation="MSTTTLTTYPGFQNNQVLTSTQLNELAAYLDQQTRLTRLRLIGL
                     GIACGLKQNFDSVTNTLTITGGSGITSEGYLIETGDCAFTQYRSYTLPATLSYPPFMD
                     GSTQVTLWELLRSDYMPVSGEIVEPLDFTFVNGTTAGDKKVVLLFLEKVFVPNDSCLG
                     KSCDETGGENTFTLRKLLVSVDDLQTILDNTGNFNLTFTGKFDLPDLLSRRPLFDPKL
                     SNSRDYFAFSQDFRDAAASVYKRSTNPLDLTDLFNVLRQCYVVFEPLLQDIYNNTNPF
                     DYT"
     gene            complement(854..>2093)
                     /locus_tag="VFU15_14400"
     CDS             complement(854..>2093)
                     /locus_tag="VFU15_14400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719030.1"
                     /translation="SGPDMGDYTVVKVVPSGLSNSLIYVKEKIVSTSPSSPFLFYTKS
                     LPIVAVNITGSNNEFFLKPGADEVAAQELADWVKAKFFSHEGMHVLEHILLRPKYNEM
                     GPPLAISPANNNQLKNTAPLGKATFIKQFSPYTVDQVNKKFTIAADYTAELLPLKTIR
                     ITGSPFNDGIYTVRFSVFTGGNTEITVYDTIPDSTPGGYLQYSMTYTIQSVGGANMDE
                     ATIDDPQFVAPANGDVIITDSFEEENNGKFHMISYTNPSGTLYTMTFDTRIAVITDDF
                     LPIELHNDCLFCRFDDPYSFIVSVILPAWQGRFFNQDFRRFFDRTLRLECPAHLILNI
                     CWLDCKQMGAFEDKYKKWLLENAKQNTDKLEVSKALNEVIAVLKDLRTVYPGGILHDC
                     TSDTGPENAVILNQTALGNL"
BASE COUNT          462 a          514 c          610 g          507 t
ORIGIN      
        1 tgtatagtcg aacgggttcg tgttattgta aatatcctgc agcagcggtt cgaaaacgac
       61 atagcactgg cggagcacgt tgaacaggtc ggtgagatcg agcgggttcg tgctgcgttt
      121 gtaaacactc gcggctgcat cgcggaaatc ctgtgagaat gcaaaataat cgcggctgtt
      181 gctcagcttc ggatcgaaaa gcgggcggcg ggaaaggaga tcgggcagat cgaatttccc
      241 ggtgaaggtc aggttgaaat tcccggtatt gtcaagaatg gtctgcaggt cgtcgacaga
      301 aacgagcagc ttgcgcagcg tgaatgtatt ctctccgcct gtttcatcac agcttttgcc
      361 gagacaggaa tcgttgggca caaaaacttt ttccaggaaa agcagcacga cttttttatc
      421 tcctgctgta gttccgttga cgaacgtaaa gtcgagcggc tccacgatct cgccggaaac
      481 cggcatgtaa tcggaccgca gcagctccca caacgtcacc tgcgtgctcc cgtccatgaa
      541 cggcgggtag gacagcgttg ccggcaacgt gtacgaacgg tactgcgtga atgcgcagtc
      601 gccggtttcg atcaggtaac cttcggaggt gatcccggat ccgccggtga tcgtcagcgt
      661 attcgtgact gaatcgaagt tctgtttcag tccgcacgcg attccgaggc cgatcagccg
      721 caggcgtgtc agccgtgtct gctgatcaag ataagcggcg agctcgttca gctgtgtgct
      781 cgtcagaacc tggttgttct gaaaccccgg gtatgtggtt aacgtcgtag tgctcatgtt
      841 ggatgcttaa tttttataga ttacctaatg ctgtttgatt aagaatgacg gcgttttcag
      901 ggccggtgtc ggacgtgcag tcgtgcagga ttccacccgg gtaaacggta cggagatctt
      961 tcagcacggc gatgacttca ttgagggctt tcgagacttc cagcttgtcc gtgttttgct
     1021 tcgcgttttc cagcagccat tttttgtact tgtcctcgaa cgcgcccatc tgcttgcagt
     1081 cgagccagca gatattgaga atgagatgcg ccgggcattc gaggcgcagc gtacggtcga
     1141 agaaacggcg gaagtcctgg ttgaaaaatc ttccctgcca cgccgggagt attacggaaa
     1201 caatgaatga atacggatca tcgaaacggc agaagagaca atcgttgtgc agctcgatcg
     1261 ggaggaaatc atcggtgatc accgcgatgc gcgtgtcgaa cgtcatcgtg taaagcgttc
     1321 ccgacgggtt cgtgtagctg atcatgtgga acttcccgtt gttctcttcc tcgaatgaat
     1381 ccgtaatgat cacatcgccg ttcgccgggg cgacaaactg cggatcgtcg atcgtggcct
     1441 catccatatt cgcgccgccg accgattgaa tcgtatacgt catcgaatac tggagataac
     1501 cgccgggagt ggaatcggga atagtgtcgt acacggtgat ctccgtgttg ccgccggtga
     1561 agacggaaaa acggaccgtg taaatgccat cgttgaaagg agagcccgtg atgcggatcg
     1621 ttttcagcgg gagcagctcc gcggtgtagt cggccgcgat cgtgaacttc ttgttcacct
     1681 ggtcgacggt atacggggaa aattgcttga tgaacgttgc tttaccaagc ggcgctgtat
     1741 ttttcagctg gttgttgttg gccggcgaga tggcgagcgg cggtcccatt tcattgtatt
     1801 tcggacgcag gagtatatgc tcgagcacgt gcatgccttc gtggctgaag aatttcgctt
     1861 tcacccagtc ggccagctcc tgtgcggcaa cttcgtcggc acccggcttc aggaagaact
     1921 cattgttcga accggtgatg ttcacggcaa cgatcgggag cgactttgta taaaacagga
     1981 acggagagga tggtgaagtg gaaacaattt tttccttcac ataaatcagc gagttgctca
     2041 gcccgctcgg aacgactttc accacggtgt aatcacccat gtcggggcca ctc
//
LOCUS       DASTSF010000503        16435 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1971761,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000503 DASTSF010000000
VERSION     DASTSF010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 16435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16435)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16435
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1971761"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1297
                     /gene="hutH"
                     /locus_tag="VFU15_14405"
     CDS             <1..1297
                     /gene="hutH"
                     /locus_tag="VFU15_14405"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018477435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HEU4719031.1"
                     /translation="LSQLQVNLVMSHACGTGDAVPVEIVRLMILLKIQGLIHGNSGVQ
                     PATVQLLVSFWNNHILPVVYELGSLGASGDLAPLAHLALPLLGKGEVNQYGTVRPAAE
                     VLHECGLQAVTLKAKEGLAILNGTQFMSAWGVWNLLHAKNILEAADAIGALSLDAYDG
                     MKEPFDARLHDIRPHPGQLASAARIAELIADSPIANKPREKKRDVQDPYSFRCMPQVH
                     GASRDAVDYAERVFLTEINSVTDNPTFFPREGDVLSGGNFHGQPLALALDFLAIALAE
                     IASISERRIFLLIAGKRGLPSMLIDNAGLNSGFMIPQYAAAAVVSQNKQLCTPASVDS
                     IVSSNGQEDHVSMGANAATKCARVTQNVERVLGIELLTAAQALDFRMKLEDARSSPRI
                     MKLQDEFRAEVFFASHDHEMYPDMAVAEKFVRDFDFSVL"
     gene            1294..1869
                     /locus_tag="VFU15_14410"
     CDS             1294..1869
                     /locus_tag="VFU15_14410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719032.1"
                     /translation="MKKLLFVFAANLFFLHAFACNCPTVALTKENLAAYDFIFTGKVV
                     AVSGCSDDASVSFEIEKLYRGKSYREAQLKFDCTSDCGMTFSPGEEWIIYAKYVGYGK
                     AEVKFCSFSRKYVADDKNDFVTPLHGMSYAMENEWLEKTLGVQELNVRNPEDDMHHEN
                     IHPSQQQALLYFGLGLAGLLVLLFLTKKFLR"
     gene            1869..2504
                     /locus_tag="VFU15_14415"
     CDS             1869..2504
                     /locus_tag="VFU15_14415"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006891258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic pyrophosphatase"
                     /protein_id="HEU4719033.1"
                     /translation="MAAAFSSFRLHPWHGISPGENAPAEVTAFIEIVPGDTVKYEIDK
                     ETGFLKIDRPQLFSNYVPALYGFIPQTYCGDSVAEHARKVAGENVQKGDGDPIDICVF
                     SERPVTHGDIILKAIPVGGIRMIDKGEADDKIIAVLKDDAAYGEVTDLAQLPQRVVDR
                     LRHYFLTYKTIPGESRGPVELAGIYGREEAQEVIRKSMEDYRALLAAQTER"
     gene            2643..7808
                     /locus_tag="VFU15_14420"
     CDS             2643..7808
                     /locus_tag="VFU15_14420"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEU4719034.1"
                     /translation="MWLAAAFQTVSAQQYNYLHYSVNNGLPQARIYSEFQDSRGYLWI
                     GTEGGVSRFDGHEFETFSVDNGFPASTVTAINEDNEGVLLGTDQGLVVFSYGKFTLYR
                     KNGFDQIHRILITPGGSALLCTNSGLVGFSENKFTQVKTSTAVDQLDIAEGLFDSRGH
                     LWIGTDMNGVFEFSCENDRYSNLVFPDQEKLVNARIRGLVEIEGGSIWIATSTDGLFS
                     FDGNALTMLNIPLSGKLFFTCAHKDIFGDVWLGTWGSGLIHYTGNVFKFYNRSNGLGD
                     DKIQCITSDQQGNTWIGTFSNGVYFFYGDQFTSLTKADGLPDNEVHGIVQDDEGNMWF
                     GTSAGLAMFDGVDVKNIGTGNADLEEGIGALATDGKQIFAGSTDGAIVVVDGNSVRSY
                     TPANEKMSEVISLLYTREGTLWIGTVSEGLFEFTGGKITRVNTGNVLLNNPIWSIYES
                     VDGVIWLGTEKGAFRMHNGSALRPESDNPSYMPDTRIYSICGDNDYIYFTSQGKGLFR
                     WSRAKKHYEVFSKREGIGSLYTTGILWMNSNTMFVTHSLGMDKIIFSSDSQQVRHFWY
                     SDGIGTDNFTPSCMYFSREGKLWLGSSNGVIIYSIGGDKGKLSAPLIEIKDVLLFNQR
                     PDWKSYSQKMLKSGLPFEPVLPYGQNQINFHLAGIQYGAGVNIQYSYMLEGLDSSWTM
                     LHDGNSISYTNLPPGDYRFLVKAGNSNNAWSVPTEFRFSIKPPFWGTWWFFIIVLSII
                     SGISLVLVFLYRRFKSDFVRRHRSFGDYQLTTSRMILLFSGILYPVSFFVVNLFPPYN
                     HANGWDVIAVGALLTFAGAATYYSPFFRKSASLITQILYPLLIFHICYLAHQDQLNPV
                     TIAMLLVALGAGGIVFDNMRSAAVFSTALLAVIGILIFTAGNNTGFNKWVLLLGTIVV
                     IIITIVSVLSRLNLFNRLVFADTTINNSRSLVIAADESGKIIFVSRSVKAILGYDEEE
                     VMGDKWWKVRTTNQDENDRMREKILASSGTQASYIAPLRAKNGSTRWIQWSDTEIGGG
                     IKVGIGLDVSDRIEIEERYRHIVESATDIIYTADYRGNLNFINDVAKKITGFSQEDLI
                     GKHFTQFVRADWVDDVKRFYEKQFSRKTVSSYYEFPIISAAGKEIWLGQTVRLLFDET
                     RPNLIRGFQAIARDITEKKRYEEELEKLSLVASETINGVLICEPDGRIEWVNDGFTRI
                     TGFSLEEVKGKMPGDVLAGDRTDWIAISEVREFSKKAEGFQKEFLVYHKNGSEIWLSV
                     SNTPIVDEHGTILNQIEIFNDITEKKRYEIQLNRYSSRLETLNMTKMELLRSVTIEEI
                     AKNVLGSLASRLPFVIRTSMALFDERDGFAEMYFVLKNDNKKLSRYSMPLNNFRSLPN
                     LYRNTHLLINDLQQEENLSESDRENISAGVRSYLVMPLYTQGKLIGSVNIGAPKAYSI
                     GEEDIEMIREMADAMAATVQQLRYLEIIEQKNEDISASILYARRIQDAILPPEEMLRD
                     QVGDLFVLHRPKDMLSGDFYWAEKKGKFTFLAVVDSTGHGVPGALLSLMGNNLLNQAV
                     HERHLVRPSAVLDYLNAGIQHTLNQYKKAGELRDGMDITLCVFEEGTNRMQYAGAINP
                     LYVIRDEMVIQSKGNRFSIGSYFDNRMRPFTNQEMELQKDDVVYLFSDGFPDQFGGTE
                     DRKLSHRRFREILLEVHKEEMSVQKRMLEEQLNMWMNGEPQTDDICVIGIRIR"
     gene            7816..8505
                     /locus_tag="VFU15_14425"
     CDS             7816..8505
                     /locus_tag="VFU15_14425"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008626714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEU4719035.1"
                     /translation="MNILYEDNHLIAVNKRPSEIVQGDKTGDKTLGDDVKEFLRRKYA
                     KPGEVFLGVIHRIDRPVSGIVLFAKTGKALSRMNEMFRTREVQKTYWAVVSPRPPADA
                     QTLVHWLRKNEASNKSFASTVESRNSLRAELDYRLLVPSDNYFLLEVLPHTGRHHQIR
                     VQLSAIGSPIKGDVKYGARRANPDASIHLHARKLEFIHPVKKEKIVITADPPEEPLWK
                     FFREEVKRLGG"
     gene            complement(8523..9224)
                     /locus_tag="VFU15_14430"
     CDS             complement(8523..9224)
                     /locus_tag="VFU15_14430"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU4719036.1"
                     /translation="MFTGSSEFYDLLYSFKDYRKEAEEITALIQSRNKDCKTVLDIAC
                     GTAEHHRFLREQFSMDGIDLNETFIETARKKNREGNYSVADMKDFTTGKKYDAVICLF
                     SSIGYLHDAGELRAAVTWFARHLDKNGLLIIEPWFTKEHSRERRTGMLTYEADDLKIC
                     RLSHSYPEGDFSVLHFEYLVATAKDGIRHFSEKHLLRMFSVGEIMQAMTEAGFETVYD
                     EKGLTGRGLYTGIKT"
     gene            9332..10156
                     /gene="ispE"
                     /locus_tag="VFU15_14435"
     CDS             9332..10156
                     /gene="ispE"
                     /locus_tag="VFU15_14435"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018342588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEU4719037.1"
                     /translation="MILYPNAKVNLGLNVISKRGDGYHNIETVFYPIPWCDILEIIPA
                     GENEKGVLLSLSGIPVPGSAEENLCRKAYALLSQHYDVPALRAHLHKRIPTGAGLGGG
                     SSDAAFFMKGVSELCDLNLSWGELHHFAKQIGADCSFFITNRPAYAQEKGDDLEGVDL
                     SLAGKYIVAAHPGIAVSTKEAYAGVVPEQPLRCVKDIVCSEPPENWKDILVNDFEKSV
                     FEKYPAIRLLKEKMYSLGALYASMSGSGSAVYGIFNDKPAYDDVFEGCSVFGEQMV"
     gene            complement(10306..12378)
                     /locus_tag="VFU15_14440"
     CDS             complement(10306..12378)
                     /locus_tag="VFU15_14440"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG-like jellyroll fold domain-containing
                     protein"
                     /protein_id="HEU4719038.1"
                     /translation="MKKTITRIVTALLCLSGVALFAQPTPDVLYYKFDGSGTSVPNMS
                     SNPPAGTTTATIMGGLSQGPTGQCGGALIGSGNAASTDYLNTNWQTNLTSASSWTISF
                     WTSNVQGTSTLYYIFGDVNANSFRCFTNGVALANNWILRGTGLNDVLATGGAQTTPTL
                     TTFVYDAVAGNIYAYVNGTLVNTVAEPGPFSFVSSGPFKVMGYSSNVGMSAGGLLDEF
                     RLYSYALTPAEVASLMTPATYNTLAVNACSSYLSPAGNTYTTSGTYMDTIPNSGGCDS
                     VITINLTVNQPSSSTMSATACGSYTAPSGAMFTSSGTYMDTIPNALGCDSVITINLTV
                     NQPSASSFSVTACSSYTSPSGMNITSSGMYMDTIPNAIGCDSVMTIDVTINNPTSSSI
                     SPMACDSFMAPSGTMYYASGTYTDIIPNAAGCDSVITISLTVNNSSASMISPVVCDSF
                     AAPSGTMYYTSGTYTDIIPNMTGCDSVITINLTVNTPSSSSINVNSCGPYTAPSGAVY
                     TSTGTYTDMIPNMAGCDSVITIALTVNSVNTSVTQAGITLTAAASGATYQWIDCSNNT
                     PVNGATSQSFTPSVDGSYAVIVMQNSCTDTSNCFSVTGVGIRENTFAGQITVFPNPNN
                     GDFTIDLGATYADAKVIVTDVTGRVVLTQDASGKQVVPVVLNAPAGVYSVEVTAGDHK
                     AVLRLVKE"
     gene            complement(12570..13256)
                     /locus_tag="VFU15_14445"
     CDS             complement(12570..13256)
                     /locus_tag="VFU15_14445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HEU4719039.1"
                     /translation="MKNNEAPLDISYLELRRAIGFMGMGLPFALIIGSAILHALDPGK
                     YCTIFQGSVSCYYHSAMRNVFEGVIWLFGIFLLFYRYEKTDTLAAKAAGICALGVANC
                     PPFVGHCDSCCITCAFDSEPVGNLHLAFAGIFFLILACMSLFLFTRTHKGKTVDRLSR
                     KWKRNVVYRICGWTIIGCLVLIAVYMLTKRSDNGSLQQLHPVFWLETVMLLAFGVSWI
                     VKGDVLLKDL"
     gene            complement(13278..15317)
                     /locus_tag="VFU15_14450"
     CDS             complement(13278..15317)
                     /locus_tag="VFU15_14450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719040.1"
                     /translation="MYKHFSSALLIMSLVAGYPALHAQVEKQFDLGSKTVRPHLRILD
                     VSYTADSLLVLSFPDSAQVVFSQKFADLGDAAAFFFFERLQPISRDSAVTRSAWKAFT
                     GEYADLREAAFWNKCQDSGVICGQYSLYPLFSSEKILDLQYDGRIFRARFPAAQYDSS
                     LYMMDLTESFLTGFIADRCLHNWNGPRIRMSDTAAKAVASVVAKQFVADMQKKKLADE
                     KNARLAQLSGDVGSIVDEMSKYQDVAFTVALKKDAIVTEYSGHRCHVIHKSDKRKKDP
                     KDQKTGDRKMVVDSVWISTNDYMISTVRVFAHDQQNPQKTTRYVNIIPLAVASMVSQM
                     KTDNSVLIEESIGHKLFGRNDINYLHFNEVMDLFPGTSTFLTDYIPEDTAFMLTRKQP
                     AAAVRKDVPASFFHIAAYTDMNGFIDNTVPNGKLQVEGYCVIPMNTRCKKMWMGNSFT
                     LFKDVLPELCLSKVGDDNRLLTVRIDTFSVDSVKHVNNLQLYQHINFWAGGRINVFTT
                     EGRNSNSTFYFNYRFRYMKCQFADTLFGNKRIFTSWAVSHEMEFYFERKLSKQFSLGL
                     VLQMDFLQLYDNSIRVSCGKIYNDDRDNRFVPETILHFNSLALLNKIWTAPQFIWTPQ
                     INLEYRSRNSPYKSAFARIAFPSSFISHNGYLVAQIGISRPLSTLFSSQKKSSEK"
     gene            complement(15518..>16435)
                     /locus_tag="VFU15_14455"
     CDS             complement(15518..>16435)
                     /locus_tag="VFU15_14455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719041.1"
                     /translation="KALLKVLKATFQKLGYEVCHSTGETPDAGGNWQRFEINRKIITV
                     AGRPSVSDGPPLPQKPDDLPPIHIWDEKLCPFADNFFHLDNYFQWIGNMDNAIASKAE
                     PNEGYYFLASQSHYPEDFPHPGNGALDKAIHKAVNLFNEQVCQLGASPMAIPLPVMLF
                     PMGSPVKGGPEEACNDIYHFLSMSYVNCIVENYMTDTGWRIVNVYLPDFRESLEEIHR
                     QSINQNIPLTQSDFRKGISPLWEVVYGEQLADDKITKESLENFIYSVQHKIAAGLYRL
                     GFTNVCFVRHRFHALSHKRGALHCITKIV"
BASE COUNT         3755 a         4257 c         4608 g         3815 t
ORIGIN      
        1 gctcagccag ctgcaggtga acctcgtgat gtcgcacgcg tgcggaaccg gcgacgccgt
       61 tcccgtggag atcgtgcggc tgatgatcct gctgaagatc cagggactca ttcacgggaa
      121 ttccggtgtg cagcccgcga cggtgcagct gctggtttcc ttctggaaca accatattct
      181 cccggttgtg tacgagctcg gttcgctcgg cgcgtccggt gatcttgccc cgctcgcgca
      241 tctcgcgctg ccgctcctcg gtaaagggga agtgaaccag tacggcacgg tccgtcccgc
      301 cgcggaagtg ctgcacgaat gcggactgca ggccgttacg ctcaaagcca aggaaggtct
      361 ggccatcctc aacggcacgc agttcatgag cgcttggggc gtctggaacc tgctgcacgc
      421 gaaaaatatt ctcgaagccg ccgatgcgat cggcgcgctt tcgctcgacg cgtacgacgg
      481 aatgaaagag ccgttcgatg cgcggctgca cgacatccgc ccgcacccgg gacagctggc
      541 gtctgccgcg cgtatcgcgg agctgatcgc ggacagcccg atcgcgaaca aaccgcggga
      601 gaagaaacgc gacgtgcagg atccgtattc cttccgttgc atgccgcagg tgcacggcgc
      661 ttcgcgcgat gcggtggact acgcggaacg cgttttcctg acggagataa attcggtgac
      721 ggataacccc acatttttcc cgcgggaagg cgacgtgctt tccggcggaa attttcacgg
      781 gcagccgctc gcgctcgcgc tcgattttct tgccatcgca ctggcggaga tcgccagcat
      841 ttcggagcga aggatcttcc tgctcattgc cggaaagcga gggcttcctt ccatgctcat
      901 cgataacgcc gggctgaatt ccggtttcat gatcccgcaa tatgcggcgg cggctgttgt
      961 cagccagaac aagcaattgt gcacgccggc ttccgtcgat tccatcgttt cctcgaacgg
     1021 gcaggaagac cacgtgagca tgggtgcgaa cgcggccacg aaatgtgcgc gcgtcacgca
     1081 gaacgtggaa cgcgtgctgg gcatcgaact gctgacagcc gcgcaggcgc tggacttccg
     1141 gatgaagctg gaggacgccc ggtcgtcgcc gcgcatcatg aagctgcagg atgagttccg
     1201 cgcggaggtt ttctttgcat cgcacgacca tgaaatgtat cccgacatgg ccgtggcgga
     1261 aaaattcgtc cgcgacttcg atttctccgt gctatgaaaa agctgctctt cgtttttgcc
     1321 gcaaacctgt ttttcctcca cgcattcgcc tgcaactgcc cgacggttgc gctgacgaaa
     1381 gagaacctcg ccgcttacga ttttattttc acgggaaaag tggtggccgt ttccggctgc
     1441 agcgatgacg cgagcgtcag cttcgagatc gaaaagctgt accgcggaaa atcgtaccgg
     1501 gaagcgcagc tgaaattcga ctgtacgtcg gattgcggga tgacgttttc tcccggagag
     1561 gaatggatca tttatgcgaa atatgtcggt tacggaaaag cggaggtgaa attctgcagc
     1621 ttcagccgta agtatgtggc cgacgacaaa aacgatttcg tgacgccgct gcacggaatg
     1681 tcgtatgcca tggaaaacga atggctggaa aaaacgctcg gcgtgcagga gctgaatgtg
     1741 cgaaacccgg aagacgacat gcaccacgag aatatccatc cgtcgcagca gcaggcgctg
     1801 ctgtatttcg ggctgggcct cgccggtctc ctcgttcttc ttttcctcac taaaaaattc
     1861 ctcaggtaat ggccgctgca ttttcttctt tccgtcttca tccctggcac ggtatttctc
     1921 ccggtgaaaa tgcgcctgcg gaagtgaccg ccttcatcga gatcgttccc ggcgatacgg
     1981 tgaaatacga gatcgacaag gaaaccggtt tcctgaagat cgaccgcccg cagctttttt
     2041 ccaattacgt acccgcgctg tacgggttca ttccgcagac ctattgcggc gacagcgtcg
     2101 cggaacatgc acgcaaggtt gccggtgaaa atgtgcagaa aggagacggc gacccgatcg
     2161 acatctgtgt tttttccgag cgcccggtga cgcacggcga cattatcctg aaagcgatcc
     2221 ccgtcggcgg catccgcatg atcgacaagg gcgaagcgga cgacaagatc atcgcggtgc
     2281 tgaaagacga cgccgcgtac ggggaggtta cggacctggc gcagcttccg caacgtgtcg
     2341 ttgaccggct gcgtcattat ttcctgacgt acaaaaccat tcccggggaa tcgcgcggcc
     2401 cggtcgagct ggcaggaatt tacggacggg aagaggcgca ggaggtgatc cggaaaagca
     2461 tggaagatta ccgtgcgctg ctggccgcac aaacggaaag atgacctccc gtcagaccgg
     2521 tttttcattg agcccgcgct gttttttcat gcaatggaat acttaccttt agaaagtcct
     2581 gagcaaaggc aatgcgcgtt tttttacccc gcataaaaac atattttttc tttggtgcac
     2641 tgatgtggct ggccgctgca tttcagacag tttccgcaca gcaatacaat tacctccatt
     2701 attcggtcaa caacgggctt ccgcaggcga ggatctattc cgagttccag gattcgcgcg
     2761 gttatctctg gatcgggacg gaaggaggcg tgtcgcgttt cgacgggcat gaattcgaaa
     2821 cgttcagcgt cgacaacggt tttcccgcca gcacggtaac ggcgatcaac gaagacaacg
     2881 aaggcgtgct gctgggaacg gaccagggac ttgtcgtttt ctcctatggg aaattcacgc
     2941 tgtacaggaa aaacggtttc gaccagatcc atcgcatact catcacgccg ggcggaagcg
     3001 cgctgctctg cacgaacagc ggcctcgtcg gtttttctga aaataagttc acacaggtga
     3061 aaacatccac cgcggtcgat cagctcgaca ttgcagaagg attgttcgac agccgcgggc
     3121 atttgtggat cgggaccgac atgaacggcg ttttcgaatt ctcctgtgag aacgaccggt
     3181 attcgaacct ggttttcccc gaccaggaaa agctggtcaa cgcgcgcata cgcgggctcg
     3241 tggagatcga aggcgggtcg atctggatcg ccacttccac cgacggactt ttttcgttcg
     3301 acgggaatgc gctgacgatg ctgaatatcc cgctcagcgg aaagctgttc ttcacctgcg
     3361 cgcacaagga tattttcggc gacgtgtggc tcggcacgtg gggaagcgga ctcattcatt
     3421 ataccgggaa cgttttcaaa ttctacaacc gctcgaacgg cctcggcgat gacaagatcc
     3481 agtgcatcac ttcggatcag cagggcaata cctggatcgg aacgttcagc aacggcgttt
     3541 acttttttta cggcgaccag ttcacgtcgc tgacgaaagc cgacggactt cccgacaatg
     3601 aagtgcacgg catcgtgcag gacgacgaag gaaatatgtg gttcggaact tccgccgggc
     3661 tcgccatgtt cgacggtgtg gatgtgaaaa atatcggcac cggtaacgca gacctggaag
     3721 aaggtatcgg cgctcttgcc acggacggga agcagatatt cgcaggaagc acggacggtg
     3781 cgatcgtcgt tgtcgacggg aacagcgtgc gctcgtacac accggccaat gaaaaaatga
     3841 gcgaggtgat cagcctgctt tacacgcgtg aagggacgtt gtggatcggg acggtttcgg
     3901 aaggactgtt cgaattcacc ggaggaaaga ttacgcgcgt caacacgggg aacgtgctgc
     3961 tgaataatcc catctggtcg atctacgaat cggtcgacgg cgttatctgg cttggaacgg
     4021 agaaaggtgc gtttcgcatg cacaacggtt ctgcgctgcg gcccgaatcg gacaatcctt
     4081 cctacatgcc ggatacgcgt atttacagca tctgcgggga caacgattac atctatttca
     4141 cttcacaggg aaaaggtttg ttccgctgga gccgggcgaa gaaacattac gaagtgttca
     4201 gcaagcgcga aggtatcggc agtctttaca cgaccgggat cttgtggatg aactcgaaca
     4261 cgatgttcgt cacgcattcg ctggggatgg acaagatcat tttcagctcc gattcacagc
     4321 aggtacgtca tttctggtac agcgacggca tcggtaccga caacttcacg ccttcctgca
     4381 tgtatttttc gcgcgaggga aagctgtggc tcggctcttc gaacggcgtc atcatctatt
     4441 ccatcggcgg cgacaaggga aaactttctg cgccgctcat tgaaataaaa gacgtgctgc
     4501 tgttcaacca gcgtcccgac tggaagtcgt attcccagaa gatgctgaag agcggacttc
     4561 cgttcgagcc tgttctcccg tacggccaga accagattaa ttttcacctg gcgggcatac
     4621 agtacggcgc cggagtgaac atccagtatt cgtacatgct cgaaggactc gattcctcgt
     4681 ggacaatgct gcacgacggc aattccattt cctatacgaa ccttcctccc ggcgattacc
     4741 gttttctcgt caaagcgggg aacagcaaca acgcatggag cgttcccacg gaattccggt
     4801 tctcgatcaa gccgccgttc tggggaacat ggtggttctt catcatcgtg ctttccatca
     4861 tttccggcat cagcctggtg cttgtttttc tttaccggcg tttcaaatcg gatttcgtcc
     4921 gccgccaccg cagcttcggc gattaccagc tgaccacgtc gcggatgatc ctgctcttct
     4981 cgggaatatt gtacccggtc tcgtttttcg tcgtgaatct tttcccgcct tacaatcacg
     5041 ccaatggctg ggacgtcatc gccgtcggtg cgctgctcac ttttgccggt gcagcgacct
     5101 actacagtcc ttttttccgg aagtcggctt cgctgatcac acagatactg tacccgctgc
     5161 tcatttttca catctgttac ctcgcgcacc aggaccagct caatcccgtg acgatcgcga
     5221 tgctgctcgt cgcgctcggt gcaggcggca tcgtgttcga caatatgcgt tcggctgcgg
     5281 tattttccac cgctcttctc gccgtgatcg gcattctcat tttcacggcc gggaacaaca
     5341 ccgggttcaa caaatgggtg ctgctgctgg gaacgatcgt cgtgatcatc atcacgatcg
     5401 tcagtgttct ttcccgtctc aatcttttca accgcctcgt ttttgccgat acgacgatca
     5461 ataattcgcg cagtcttgtg atcgcagcgg atgaaagcgg gaaaatcatt ttcgtcagcc
     5521 gcagtgtgaa agccattctc ggttacgatg aagaagaagt gatgggcgat aaatggtgga
     5581 aggtgcgcac gacgaaccag gacgagaacg accgcatgcg tgaaaagata cttgcctcga
     5641 gcggaacgca ggccagctac attgcgccgc tgcgcgcgaa gaacggatcg acgcggtgga
     5701 tacaatggtc agacacggag atcggcgggg gaataaaggt cggcatcggt ctcgacgtca
     5761 gcgaccgcat cgaaatagaa gagcgctacc gccacatcgt ggaatcggcg accgatatta
     5821 tttacacggc cgattaccgc ggtaatctca atttcatcaa cgacgtggcg aagaagatca
     5881 ccggcttcag ccaggaagac ctgatcggga aacattttac gcagttcgtg cgtgccgact
     5941 gggtagacga cgtgaagaga ttttacgaaa agcagttctc gcgcaaaacc gtcagcagct
     6001 attacgaatt cccgatcatt tccgccgccg ggaaagagat ctggctcggg cagacggttc
     6061 gccttctttt cgatgagacg cgcccgaacc tcatccgcgg gttccaggcg atcgcacgcg
     6121 acatcaccga gaagaaacgt tacgaagaag agctggaaaa actttcgctt gtcgcgagtg
     6181 aaaccatcaa cggcgttctt atctgcgaac ccgacggaag gatcgagtgg gtgaacgacg
     6241 gcttcacgcg catcaccggc ttttcgctgg aagaagtcaa aggaaagatg cccggcgatg
     6301 tgctggcggg cgatcgtaca gactggatcg cgatctccga agtgcgggaa ttctcaaaaa
     6361 aggcggaagg tttccagaaa gaatttctcg tctaccacaa gaacggaagc gagatatggc
     6421 tttcggtttc caacacgccg atcgtcgacg agcacggaac cattctcaac cagatcgaaa
     6481 tattcaacga catcacggag aaaaaacggt acgagataca gcttaaccgc tacagctcgc
     6541 gcctcgaaac gctgaacatg acgaagatgg aactgctgcg ttccgtcacg atcgaagaga
     6601 tcgcgaagaa cgtgctgggc agcctcgcct cgcggcttcc gttcgtcata cgcacatcga
     6661 tggcgctgtt cgatgaacgc gacggctttg cagaaatgta tttcgtgctg aagaacgaca
     6721 acaagaaact ttcacgctat tccatgccgc tgaataattt ccgcagcctt ccgaacctgt
     6781 acaggaatac gcacctgctc atcaacgatc ttcagcagga ggaaaatctt tccgaatcgg
     6841 accgcgaaaa tatttctgcc ggtgtgcgat cgtatctcgt catgccgctg tacacgcagg
     6901 gcaagctcat cggatccgtg aacatcggtg cgccgaaagc gtattcgatc ggggaggagg
     6961 acatcgagat gatccgcgaa atggcggatg cgatggcggc caccgtgcag cagctccgtt
     7021 acctcgagat catcgagcag aagaacgagg atatttcggc gagcatcctg tacgcgcgcc
     7081 gtatccagga cgcgatcctt ccgccggaag aaatgctccg cgaccaggtg ggcgacctgt
     7141 tcgtactgca ccgcccgaaa gatatgctgt caggcgattt ttactgggcg gagaaaaaag
     7201 gaaagttcac tttcctcgcc gtcgtcgatt ccacgggcca tggtgttccg ggtgcgctcc
     7261 tttcgctcat ggggaacaat ctcctcaacc aggcggtgca cgaaagacat cttgtacgtc
     7321 cttccgctgt tctcgattac ctcaacgcag gcatccagca tacgctcaac cagtacaaga
     7381 aagcgggcga gctccgcgac gggatggaca tcacgctctg cgtgttcgag gaaggaacga
     7441 acaggatgca atacgccggc gccatcaacc cgctctatgt gatccgtgat gaaatggtga
     7501 tccagtcgaa agggaaccgg ttcagcatcg gcagctattt cgacaaccgc atgcgcccgt
     7561 tcaccaacca ggaaatggag ctgcagaaag acgatgtcgt ttatcttttc agcgatggtt
     7621 tccctgacca gttcggggga acggaagacc ggaaattatc gcaccgccgc ttccgcgaga
     7681 tactgctcga agtgcacaag gaagagatgt ccgtgcagaa acgcatgctc gaggagcagc
     7741 tgaacatgtg gatgaacggg gaaccgcaga ccgatgacat ctgcgtgata ggtatccgga
     7801 tacgctgaca tggaaatgaa tatcctttac gaagacaacc atctcatcgc cgtgaacaaa
     7861 cggccttcgg agatcgtgca gggcgacaaa acgggcgaca agacgctcgg cgacgacgta
     7921 aaggaattcc tgcgcaggaa atatgcgaaa ccgggagagg tttttctcgg cgtcattcat
     7981 cgcatcgacc ggccggtgag cggtatcgtg ctgttcgcaa aaacagggaa ggcgctttcg
     8041 cgcatgaatg aaatgttccg gacgcgcgaa gtgcagaaaa cgtattgggc ggtcgtttct
     8101 ccacgtcctc ctgcggatgc gcagacgctt gtgcactggc tcaggaaaaa tgaagcgtcg
     8161 aacaaatcat ttgcgtcgac cgtcgaatcc cggaattcgc ttcgtgcgga gctcgactat
     8221 cgcctgctgg ttccttcgga taattatttc ctgctcgaag ttcttccgca caccgggcgt
     8281 catcaccaga tccgtgtgca gctgtcggcc atcggttcgc cgatcaaagg cgatgtgaaa
     8341 tacggtgcgc gccgcgccaa tcctgatgct tcgatccatc ttcatgcgcg aaagctggag
     8401 tttattcatc cggtaaaaaa agaaaagatc gtcatcaccg ccgatccgcc ggaagagccg
     8461 ctgtggaaat ttttcaggga ggaagtgaag aggttgggag gttgaaaggt tgggaggtgt
     8521 cgtcatgttt ttatcccggt gtagagtccg cgcccggtta atcctttttc atcgtaaaca
     8581 gtttcgaagc cggcttccgt catggcctgc atgatctcac cgacagagaa catgcgaagg
     8641 agatgctttt ccgaaaaatg ccggatgccg tctttcgccg tcgcgacgag gtattcaaaa
     8701 tggagaacgg aaaaatcgcc ttcgggataa gaatgcgaca ggcggcagat cttcaggtca
     8761 tctgcttcat atgtaagcat gcccgtgcgt ctctcccgtg agtgctcttt cgtgaaccag
     8821 ggttcgataa tgagcagccc gtttttatcc aggtgccttg caaaccaggt tactgctgcc
     8881 cggagctcgc cggcatcgtg caggtaaccg atggagctga agaggcagat gaccgcgtcg
     8941 tattttttcc cggttgtaaa gtctttcatg tctgcgacgg aataatttcc ctcccggttt
     9001 ttctttcgcg ccgtctcaat gaacgtttcg ttgagatcga tgccgtccat ggaaaattgt
     9061 tcccgcagga agcggtgatg ttccgcggtt ccgcacgcga tgtcgagaac cgttttacag
     9121 tctttgttcc tggattgaat aagcgccgtg atctcctccg cttccttcct gtaatccttg
     9181 aagctgtata ggagatcgta aaattccgag ctgccggtga acatgaaatt cgtttagcgt
     9241 gttgcgaagg aaatgattcg gtgacgttga atgctaaata taaagcattg tgccgtggat
     9301 ttttcagccg cttttttgaa tctttacagc gatgatcctt tatcccaatg caaaggtcaa
     9361 cctcggactg aacgtgatct caaaacgcgg tgacggttac cacaacatcg aaacggtttt
     9421 ttatcccatt ccgtggtgcg atatcctgga gatcattcct gcgggtgaaa atgaaaaagg
     9481 tgtgctcctt tcgctgagcg gaattcccgt tcccggaagc gcggaagaaa atctctgccg
     9541 gaaagcttac gcgctgcttt cgcagcatta tgatgtcccc gctttgcgtg cgcaccttca
     9601 caagcgcatt cccacaggcg ccgggctggg cggcggttct tccgacgctg cttttttcat
     9661 gaaaggcgtc agtgaactgt gcgatctcaa tctttcctgg ggcgaactgc atcatttcgc
     9721 gaagcagatc ggcgccgatt gttctttctt catcaccaac cgtcctgcgt atgcgcagga
     9781 gaaaggagat gacctcgaag gtgttgatct ttcgcttgcc gggaaataca tcgtggctgc
     9841 gcaccccggt atcgcggtga gcacgaagga agcgtacgcg ggagttgtcc cggagcaacc
     9901 gctgcgttgc gtgaaggata tcgtttgcag cgagccgccg gaaaactgga aagatattct
     9961 tgtcaatgat tttgaaaaaa gtgtatttga aaaatatcct gcgatacgcc tgctgaagga
    10021 aaaaatgtat tcgctgggcg cgctctatgc ttccatgagc ggaagcggat cggcggtgta
    10081 cgggattttc aatgataagc cggcttatga tgatgtgttt gaaggatgtt ctgtgttcgg
    10141 ggaacagatg gtgtgatctc ccgggatgca tgaaggaaag aactgcggtt ccggaataac
    10201 cgcgaagccc gtaaaacgcc tgcggcggat cccggacatc ctggatggat ccaaaagaaa
    10261 agcgtcccgt ttgaggacgc ttttcttttt atgaaaaccc ggttcttatt ctttcacgag
    10321 gcgcagaacc gctttgtgat cacctgcggt gacttccacg gaataaacgc ccgcgggtgc
    10381 attcagtacc acaggaacaa cctgtttgcc ggaagcatcc tgcgtgagta caactcgtcc
    10441 cgttacatcg gtaacgatca ctttggcatc agcatacgtt gcgccgaggt cgatcgtgaa
    10501 atcgccgttg ttcgggttcg ggaaaacggt gatctgtcct gcgaacgtat tttcgcggat
    10561 accgacgccc gttacgctga agcagttcga cgtgtccgtg cagctgttct gcatgacgat
    10621 cacggcgtag cttccgtcaa ccgatggcgt gaacgattgt gaggtcgcgc cgttcaccgg
    10681 tgtattgttc gaacaatcga tccactggta cgttgcgccc gaagcggcag cggtcagggt
    10741 gatgcccgcc tgtgttacgc tggtgtttac gctgttcacc gtaagtgcaa tcgtgataac
    10801 gctgtcgcag cccgccatat tcgggatcat gtcggtgtaa gttcctgtcg aggtgtaaac
    10861 cgcaccgctc ggcgcagtgt aaggtccgca ggagttgaca ttgatggaag aagaggaagg
    10921 cgtgtttaca gtaaggttga tcgtgatgac gctgtcgcat cccgtcatgt tcggaataat
    10981 atccgtgtat gttccggacg tgtaatacat tgttccgctc ggagctgcaa agctgtcgca
    11041 gacgacaggc gaaatcatcg acgctgaaga attgttcacc gtgaggctga tggtgatgac
    11101 gctgtcgcaa ccggctgcgt tcggaatgat atcggtgtac gttcccgaag cgtaatacat
    11161 cgttccgctt ggcgccatga agctgtcgca ggccatcggg gatatcgaag aagacgtcgg
    11221 gttatttatc gtcacgtcga tcgtcatgac actgtcgcac ccgatcgcat tcgggatcgt
    11281 atccatatac atcccgctcg aagtgatatt cattccgctc ggggatgtat acgaagaaca
    11341 cgccgttacg ctgaacgaag aggcgctcgg ctggttgacc gtgaggttga tggtgataac
    11401 actgtcgcag cccaacgcat tcgggatcgt gtccatatac gttccgctcg atgtgaacat
    11461 cgcgccgctg ggagccgtgt agctgccgca ggccgtggcg gacatcgtgg aggaggaagg
    11521 ctggttgacg gtaaggttga tggtgatgac gctgtcgcat ccgccggaat tcgggatcgt
    11581 atccatgtac gttccggatg tcgtgtacgt attgccggcc ggggaaaggt aactggagca
    11641 ggcattcacc gcaagcgtat tgtacgttgc aggagtcatg agcgatgcaa cttcagctgg
    11701 tgtcagcgcg tagctgtaaa ggcggaattc atcgagtaat ccgccggcgc tcatgccgac
    11761 gttggagctg tatcccatca ccttgaaagg cccggagctg acgaacgaga aggggcccgg
    11821 ctcggcaacg gtattcacga gagtgccgtt tacatacgcg tagatgttac cagcaaccgc
    11881 atcgtacacg aaggttgtga gcgtcggtgt ggtctgcgcg ccgccggtgg cgagcacgtc
    11941 gttcaggccg gtgccgcgca ggatccagtt gttggccaat gccacaccgt tggtgaagca
    12001 gcggaagctg ttggcattca catcaccgaa aatataatag agcgtggaag ttccctgtac
    12061 attcgaggtc cagaaggaaa tcgtccagga agacgcgctg gtaaggtttg tctgccagtt
    12121 ggtgttgagg taatccgtcg aagcggcatt tccgctgccg atcagcgcac cgccgcactg
    12181 gcccgtgggt ccctgcgaaa ggccgcccat gatggtggcc gtggtggttc cggccggagg
    12241 attgctcgac atgttgggaa cggaggttcc gcttccgtca aatttgtaat agagcacatc
    12301 cggcgtaggc tgggcgaaga gcgctacacc cgaaaggcaa agaagagctg ttacaatgcg
    12361 ggtaattgtt tttttcatac gttgattttt agtggaggtc tgcgggaatt ttgaattgcc
    12421 aaaaataagt aaaaaaatcc cgcgcacctt tcggcacgcg ggatccggga aattttcagc
    12481 ccggaatggt cagcgcgtta tgttcatctg cttcgccgca acagccatgc tgccacgccc
    12541 gccccggaaa cacgaaggtg caacccggat cacaggtcct tcagcagcac atctcctttc
    12601 acgatccagg atacgccaaa agcgagaagc ataacggttt ccagccagaa gaccgggtgc
    12661 agctgctgaa gcgatccgtt gtcgctcctc tttgtcagca tatacaccgc gatcagtaca
    12721 aggcagccga taatggtcca gccgcagatc ctgtacacca cgttgcgttt ccatttgcgc
    12781 gacaggcggt cgacggtttt tcctttatgc gtgcgtgtga acaggaagag cgacatgcac
    12841 gcgaggatga ggaagaatat tcccgcgaaa gcaagatgga ggtttccgac cggctcgctg
    12901 tcgaatgcgc atgtgatgca gcaggaatcg caatgcccga cgaacggcgg acagttggcc
    12961 actcccagcg cgcagatgcc ggctgctttc gctgcgagcg tatctgtttt ttcataccgg
    13021 taaaacagga ggaagatgcc gaacagccag atgacgcctt cgaaaacatt ccgcatggcg
    13081 ctatggtaat aacagctgac cgaaccctgg aatatggtgc agtattttcc cgggtccagc
    13141 gcatgcagga tcgctgagcc aatgataagc gcgaagggca ggcccattcc cataaagccg
    13201 attgcccggc gcagttcgag atacgagata tccagcggag cttcgttgtt tttcatagaa
    13261 aagaatttta gttccggtta tttttcagag gattttttct ggctgctgaa gagcgtggac
    13321 aagggacggg atatcccgat ctgcgccacg aggtacccgt tgtgcgaaat aaaacttgac
    13381 gggaacgcga ttctcgcgaa agcggattta tagggagaat ttcgcgacct gtactccagg
    13441 tttatctgtg gtgtccagat aaactggggc gcggtccata tcttattcag caaagccagg
    13501 ctgttaaaat gcaggatggt ttcaggaaca aaccggttat ccctgtcgtc gttatagatc
    13561 tttccgcagg aaaccctgat ggaattatcg taaagctgga ggaaatccat ctggagaacg
    13621 agcccgagag aaaattgttt ggacagcttc cgttcgaagt agaattccat ttcgtgtgaa
    13681 accgcccacg aggtaaatat tcttttgttc ccgaaaagcg tgtcggcaaa ctggcatttc
    13741 atatacctga aacggtaatt gaaataaaag gtcgaattgg aattgcgtcc ctctgttgta
    13801 aacacgttga tgcgtccccc ggcccagaag ttgatgtgct ggtaaagctg gagattattc
    13861 acatgtttta cggagtcgac tgaaaaagtg tcgatgcgga cggtcagcaa cctgttgtcg
    13921 tctcccactt tgctgaggca aagctcgggg agcacatctt tgaaaagtgt gaaggaattc
    13981 cccatccaca tttttttgca ccgcgtattc atgggaatta cgcagtaccc ttccacctgg
    14041 agttttccgt tcgggaccgt attgtcgata aacccgttca tgtcagtgta cgcggcgatg
    14101 tgaaaaaagc tggccggcac atctttccgg accgcggcgg caggctgttt ccttgtaagc
    14161 atgaatgccg tgtcctcggg gatgtagtcg gtgagaaaag tactcgttcc cgggaaaaga
    14221 tccatcacct cgttgaagtg caggtaattg atatcattcc tgccgaaaag cttgtgcccg
    14281 atcgattctt ctatcagcac ggaattatcc gttttcattt gcgagaccat cgaggcaacg
    14341 gcaagcggta tgatgtttac gtaccgcgtg gttttctgcg ggttctgctg gtcgtgtgca
    14401 aatacgcgca ccgtggagat catgtaatca ttggtgctta tccacaccga atccaccacc
    14461 attttccgat cgccggtctt ttgatccttc ggatctttct ttcgtttgtc gctcttatgt
    14521 atcacatggc acctgtggcc ggaatattcc gttacgatcg catctttttt cagcgcaacc
    14581 gtaaaggcga cgtcctggta ttttgacatt tcatcgacga tggaaccgac atcaccgctc
    14641 aattgtgcga gacgtgcatt cttttcatcc gccagttttt tcttttgcat gtcggcgaca
    14701 aactgctttg ccaccacaga agcaacggct ttcgccgcag tgtcgctcat cctgatgcgc
    14761 gggccattcc agttgtgcag gcagcgatcc gcaataaacc cggtgaggaa actttcggtg
    14821 aggtccatca tatacaggga agaatcgtat tgcgctgcgg gaaacctcgc ccggaaaatc
    14881 ctgccgtcgt attgaaggtc aaggatcttt tctgatgaaa acagcgggta cagtgaatac
    14941 tgtccgcata ttactccgga gtcctggcat ttattccaga aagcggcttc gcgcagatcc
    15001 gcgtattccc cggtgaatgc tttccacgca gatcgggtta ccgcggaatc gcgcgagatg
    15061 ggttgcagcc gctcgaagaa gaaaaatgca gcggcatctc ccaggtcggc gaacttttga
    15121 ctgaacacaa cttgcgcgct gtcgggaaag ctaagcacaa gcagtgagtc cgcggtgtaa
    15181 ctcacgtcca gtatccggag gtgcggacgg acggttttgg acccgaggtc gaattgtttt
    15241 tccacttgtg catgcaaagc cgggtaaccc gccaccaggg acatgatgag cagggcagag
    15301 gagaagtgtt tgtacatgga gctggtttta tgtgggtttg ttcaatgtcc cggttggttt
    15361 tgaatttcac ctgcctggtt ccctttccgg cttgcggggc gtattgacag agcaatgttt
    15421 tttctgctta caacctgcaa cgggaattct ttcgtgcggc gggtattaaa attttttgcc
    15481 ggtaatgttt gagcttttcc gggataccgg gaagccctta cacaattttt gtaatacaat
    15541 gcagcgcgcc ccgcttgtgg gaaagtgcat ggaagcgatg acgcacaaaa cacacattgg
    15601 taaagccaag ccggtaaagc ccggcagcaa ttttgtgctg gacgctatag ataaaattct
    15661 ccagggattc cttcgttatc ttgtcatcgg caagttgttc tccatagacg acttcccaca
    15721 agggactgat tcctttccgg aaatcgcttt gggtaagggg aatgttctga tttatagatt
    15781 gccggtgaat ttcctccaga gattccctga aatccggcag gtaaacattg actattcgcc
    15841 acccggtatc ggtcatataa ttctcgacga tacagtttac gtaactcatg ctcaggaaat
    15901 ggtatatatc attgcaggcc tcttccggcc cgcctttcac aggacttccc attggaaaca
    15961 gcataacggg aagagggatg gccattggtg aggcgcctaa ttggcatacc tgctcgttaa
    16021 acaagttgac ggctttgtgt atggctttgt cgagagcacc attacccgga tgagggaagt
    16081 cctccgggta atggctttgg ctggcaagga aataataccc ttcgtttggt tccgctttcg
    16141 aagcgatggc attgtccata tttcctatcc actggaaata attgtcgagg tggaaaaagt
    16201 tatcagcaaa cgggcacagt ttttcgtccc agatatgaat gggcggaaga tcgtcgggct
    16261 tctggggtag tggcgggcca tcagagactg atgggcgtcc tgcaaccgtg atgattttcc
    16321 ggttgatctc gaagcgctgc cagtttccgc cggcgtcggg tgtttcgccg gtggaatggc
    16381 atacttcgta acccagcttc tgaaaagttg ctttgagcac cttgagcagc gcttt
//
LOCUS       DASTSF010000504         5902 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1123665,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000504 DASTSF010000000
VERSION     DASTSF010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5902
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1123665"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            105..1310
                     /locus_tag="VFU15_14460"
     CDS             105..1310
                     /locus_tag="VFU15_14460"
                     /inference="COORDINATES: protein motif:HMM:NF012640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HEU4719042.1"
                     /translation="MTSLSEIVHCSGHFPFACVLAANEAPERKFDICAGFSLSAERAE
                     TGFPSFPRTHQFLFGHLSYDLKNHVEKLHSAYTDRIGFPGYGFFAPAYFFRASGDDIV
                     SPGEDFFKAYSAKQNSPREKFPREKIRHRTTKEKYISDVTKLKEHIRRGDIYEINYCI
                     EFFAENVAIDPPSLFLRLNEISRAPFSALYRWKNAWLVCASPERFLEKTGDRILSQPI
                     KGTRPRGKNEKEDAGLAEELLNDPKERSENVMIVDLVRNDLSKIAARGSVKVDELFGI
                     YSFPNVHQMISTVSANVAEGKSTEDILRATFPMGSMTGAPKIRAMQLAEESENMRRGL
                     YSGSVGYFTPEGDFDLNVVIRSIQWNAATGYLSFMTGSAITANCDPEKEYEECLLKAQ
                     TLLRALGAD"
     gene            1332..2627
                     /gene="tilS"
                     /locus_tag="VFU15_14465"
     CDS             1332..2627
                     /gene="tilS"
                     /locus_tag="VFU15_14465"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEU4719043.1"
                     /translation="MLSKESFRESLYGRCGLAQGETVLAGVSGGIDSMVMAWLLHAGE
                     IPFAVAHVNYGLRGSESDEDEKFVAELAAEFSVPFYLKKCTAEEISPEDSVQQKARKI
                     RYRFFENVRDVEGMSRIATAHNLNDSVETALLNFARGSGLKGLGGIAPVREKVIRPLL
                     SFTRADILEFARKNKIRWREDSSNTTDHYTRNRFRHHLLPELANEIPQGFSGFEATFG
                     HVADSRDMISAAMKRWESECCTTKENAVRISIGAMKNWENSILFLEYFLKEKGFARCE
                     RKEILSVLEGEKGKKAVAGELALTKEIDSLVLKNSAGEPDPSTCFTLREEKFSGTVPS
                     GKWVAAVDAKKLNGPPHIRCWRTGDSMRPLGLHGKKKVSDILNELKIPSHLKRSAPVV
                     VSGDEIIWVPGYRIAEKFRVTERTENVLLLEFNPSFFHG"
     gene            2620..3150
                     /locus_tag="VFU15_14470"
     CDS             2620..3150
                     /locus_tag="VFU15_14470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719044.1"
                     /translation="MDKPEVLFEEKQFIGMNRHSILRRLLFSVFCFVAYYWSENPKPV
                     NLALFRIGEYPGKDHSGQLFFVLGISILFLSALLLFIIHMRTKVIPTAIIIDGFLSAR
                     KVRIDLSGISEVKKIKLKPSFFNRPVYNLHNKGRIRFYTHGNEAIELLTRDGIIYRIG
                     TQRPDELKRAIEEQKN"
     gene            3255..3548
                     /locus_tag="VFU15_14475"
     CDS             3255..3548
                     /locus_tag="VFU15_14475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719045.1"
                     /translation="MIQIAEFPVLIDKSLVINLSFPVDEVLPETDKIAKRRHDLMKAL
                     SLGNLDHAKVKILFRDNQGLKVVETTVWAVTDQRIVLKSGTTMPIHRIVEVII"
     gene            3658..4146
                     /locus_tag="VFU15_14480"
     CDS             3658..4146
                     /locus_tag="VFU15_14480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719046.1"
                     /translation="MKSPGKITSLGMVVVLILLTSLSFGFRNSQNAVRAKWTAYVIHT
                     ADGQADIHFKADIPGGWRMYSQNMTNTDGPLPTSIDFDPNPSYSVVGSPIESGKYTSF
                     YQPELGMEVNCLEGTANYVQHITYSEKTTFAIKCMINYMLNRDGEILPPDDEDFTITV
                     EP"
     gene            4253..>5902
                     /locus_tag="VFU15_14485"
     CDS             4253..>5902
                     /locus_tag="VFU15_14485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017256984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HEU4719047.1"
                     /translation="MKYFYRNFFLSVLFLSVTHLFAQQPPNFHVTWSYSSKKISDCEY
                     DLVFKATIEKGFHLYSQIPTDNGPIPTSFTFDASSDYELVGKTSEPKPIEKAEPVFDN
                     QVIKFFENEAEFHQRIRVKSNKVFKVTGVIDGMACNDGMCVNFYPQPVFSFSIDGTAC
                     SGSTGATGSTGTNGAGGATGKTGATGGTGTAGMPGATGPTGIMNPVLPQDQNSGGCNC
                     KNEIQAAIAGAMGNHSGHPVVDTAGCKFMLVKPSDAELHGTDAADTKSFWLVFILGFL
                     GGLTALITPCVFPMIPLTVSFFTKRSKDRKTGLRNAFTYALSIIVIYVLLGMLITIPL
                     GPDALNAMASSAVFNLIFFIVFIIFAMSFLGAFEITLPSGFINRIDKASDRGGMIGIF
                     FMAFTLCLVSFSCTGPIIGTLLVEAAHGGSYLGPAIGMTGFALALALPFALFAMFPGW
                     LNSLPKSGGWLNSVKVTLGFVEIALAMKFLSTVDLAYHWGFFKREIFLGVWIITAMLL
                     GLYLLGKLKLSHDSDIQHISTGRLMMALLSFTFALYLVPGLW"
BASE COUNT         1414 a         1587 c         1516 g         1385 t
ORIGIN      
        1 ggtaaggcaa tcacactgcg acctgttcat cgtccatgta tttgagtaat attgagctcc
       61 gttcgcggat gcgccggtta tatcgtttta gctgaaaaca tagcatgact tccctgtctg
      121 agatcgttca ctgctccggc cattttcctt tcgcctgcgt gctcgcggcg aacgaagccc
      181 cggaacggaa attcgacatc tgcgcaggtt tttccctttc ggctgaaaga gcagaaaccg
      241 ggtttccgtc ttttccacgc acacatcaat tcctgttcgg tcatctttcc tacgacctga
      301 agaaccacgt ggaaaagctg cattccgcct acacggacag gatcgggttt cccggttacg
      361 gctttttcgc tcccgcctat ttcttccgcg cttccggtga tgacatcgtt tccccgggcg
      421 aagatttttt caaagcatat agtgcgaaac aaaattctcc gcgggaaaaa ttcccgcggg
      481 aaaagatccg tcatcgcacc acgaaagaaa aatacatttc ggatgtgacg aagctgaagg
      541 aacacatccg ccgcggtgac atttatgaaa taaattactg catagaattt tttgcagaga
      601 atgttgccat cgatccgcct tccctgtttc tgcggctgaa tgaaatctcg cgcgcgccgt
      661 tttccgcgct gtaccgctgg aaaaatgcgt ggctggtctg cgcaagcccg gaacgcttcc
      721 tggaaaaaac cggcgaccgc attctctcgc agccgatcaa aggaacaaga ccacgcggga
      781 aaaatgaaaa ggaagatgcg ggtcttgcag aagagctgct gaacgatccg aaagagcgca
      841 gcgagaacgt catgatcgtg gatcttgtgc gcaacgacct gtcgaagatc gcagcaagag
      901 gaagcgtgaa agtcgatgag ctgttcggca tctattcctt tccgaacgtt caccagatga
      961 tctccacggt cagcgcaaac gtggcggaag gaaaaagcac ggaagacatc ctgcgcgcca
     1021 cctttcccat gggctccatg accggcgcac cgaagatccg cgccatgcag ctggcggaag
     1081 aatctgaaaa catgcggcgc gggttgtatt ccggttccgt cgggtatttc acaccggaag
     1141 gcgatttcga cctgaacgtc gtcatccgtt ccatccagtg gaacgccgcg acaggttatc
     1201 tctcctttat gaccggcagc gcgatcacgg cgaactgcga cccggaaaaa gaatacgaag
     1261 aatgcctgct caaagcgcaa acgctgctgc gtgcgctcgg cgccgactga aaaagggcgt
     1321 aatttcacgc catgctttcg aaggaaagtt tcagggaatc actttatggc cgttgcggtc
     1381 tcgcacaggg agaaacggtt ctcgccggcg tcagcggcgg gatcgattcg atggtgatgg
     1441 cgtggctgct gcatgcgggt gaaattccgt ttgccgttgc gcatgtgaat tacgggctgc
     1501 gcggaagcga atcggatgaa gatgaaaagt ttgtggcgga gcttgcggct gaattttccg
     1561 ttccgttcta cctgaaaaaa tgtacggcgg aagaaatttc cccggaggat tccgttcagc
     1621 agaaagcgcg gaagatccgc taccgttttt ttgagaacgt acgcgacgtc gaagggatga
     1681 gccgcatcgc cacggcgcac aacctgaatg attccgtgga aaccgcgctg ctgaatttcg
     1741 cgcgcgggag cggactgaaa ggtctcggcg gcattgcacc ggtgcgcgaa aaagtgatcc
     1801 gcccgctgct ttcattcacc cgtgcagata tcctggaatt tgcccgcaaa aacaagatcc
     1861 gctggcgcga agattcttcc aacacgacgg atcattatac gcgcaaccgg ttccgtcatc
     1921 atcttcttcc ggaactggcg aacgaaatcc cccagggctt ttcgggtttc gaagcgacgt
     1981 tcggtcatgt ggcggacagc cgggacatga tctcagcggc aatgaaacga tgggagagcg
     2041 aatgctgcac gacaaaagag aacgccgtcc gcatcagcat cggcgcgatg aagaattggg
     2101 agaacagcat tcttttcctg gaatattttc tgaaagaaaa agggttcgcc cgctgtgaaa
     2161 gaaaagagat cctgtccgtt ctcgaaggag aaaaaggaaa aaaagcagtt gcaggcgagc
     2221 tggcgctgac gaaagagatc gattcgctcg tgctgaaaaa cagcgcggga gaaccggatc
     2281 cctcgacctg cttcactttg cgcgaggaaa aattttcagg aacagttcct tccggaaaat
     2341 gggtcgctgc agtggatgcg aaaaaattaa acggtcctcc gcatatccgt tgctggcgca
     2401 ccggcgactc catgcgtccg ctcgggctgc acggaaagaa aaaagtgagc gatattctca
     2461 acgagctgaa aatcccgtca cacctgaaaa gatcagcccc cgtggtggtt tccggcgacg
     2521 agatcatctg ggttcccggc taccgcatcg ctgaaaagtt cagggtaaca gagcgcacgg
     2581 aaaacgtcct gctgcttgaa ttcaatcctt ctttttttca tggataagcc ggaggtatta
     2641 ttcgaggaga aacaattcat cgggatgaac cggcattcga tcctgcgccg cctgctgttt
     2701 tccgttttct gctttgtcgc ttattattgg tcggagaacc ccaagccggt aaacctcgcg
     2761 cttttccgca tcggtgaata tccggggaag gatcattcgg gtcagctctt tttcgtgctg
     2821 ggaatttcca tcctctttct ttccgcgttg ctgctgttca tcatccacat gcgtacgaag
     2881 gttattccca cggccattat catcgacggc ttcctgtcgg cccggaaagt caggatcgat
     2941 ctcagcggca tttcggaagt aaaaaaaata aagctgaagc cttccttctt caaccgcccg
     3001 gtgtacaacc tccataataa aggaaggata cgtttttata cgcacgggaa cgaggccatc
     3061 gagctgctta cgcgcgacgg catcatttac cgtatcggca ctcagcgacc cgacgagctg
     3121 aaaagagcca ttgaggaaca gaaaaactga gaagggacgt ttccattcat cgaatttcag
     3181 caggaaggtg aataacctgc ggaacttttg cccggcctgc ccgcgtataa tcaggcaaac
     3241 cacacaccac tcccatgata cagatcgccg aattcccggt tcttatagat aagagcctgg
     3301 taatcaacct ctccttcccg gtggatgaag tcttgccgga aactgacaaa attgcaaaac
     3361 gaaggcacga ccttatgaaa gccctttcgc tgggcaacct ggaccacgcc aaagtgaaga
     3421 tattatttcg tgataatcaa ggacttaaag ttgtcgagac aactgtctgg gcggttacag
     3481 accagcggat cgtgctaaag agcggcacca cgatgcccat ccacagaatc gtggaagtta
     3541 taatttgaaa agccctgttc ggctattttt tccagatttt tgtaacaagt cattgttaat
     3601 aaccgtttag gtggattacc tttaccaagg cagttaacct gaaaacctaa ccttattatg
     3661 aaatctcccg gaaaaatcac cagcctcggc atggtggtcg tgctgatcct gctgacttcg
     3721 ctttctttcg gtttccgaaa ttcccagaac gcggtcaggg ccaaatggac ggcttatgtc
     3781 atccatacag ccgacggcca ggcggatatt cacttcaaag cggatattcc cggcggatgg
     3841 cgcatgtatt cacagaatat gacgaatacg gacggacctc ttccgacgag catcgacttc
     3901 gatccgaatc ccagctattc tgttgtgggt tctccgatcg aatccggcaa atacacctcc
     3961 ttttaccagc ctgaactggg aatggaagta aattgcctcg aaggaactgc gaattatgtt
     4021 cagcacatca catactcgga aaagacgacg ttcgccatca aatgcatgat caactatatg
     4081 ctcaaccgcg acggcgaaat acttcccccg gacgatgagg attttacaat tactgtagaa
     4141 ccttaacatt ttttgcccga tcatcagcgc agattgatct attttcggtc gtgagcggaa
     4201 agcccttatt tttgggaatc ccctgcgacc gaaaattttt tatgcccgtg ctatgaagta
     4261 tttctaccgg aattttttcc tctccgtcct ctttctctcc gttactcacc tgtttgcgca
     4321 gcagccgccg aattttcacg taacgtggag ctactcttcg aaaaagatca gcgattgcga
     4381 atatgatctc gttttcaaag cgacgatcga gaaaggattt cacctctatt cacagatccc
     4441 gacggacaac ggtcccattc ccacttcttt cacgttcgat gcaagcagcg attatgagct
     4501 ggtcgggaaa acctccgaac cgaagccgat cgaaaaagcg gaacctgttt tcgataacca
     4561 ggtgatcaaa tttttcgaga acgaagctga attccaccag cgcatccgcg tgaagtcgaa
     4621 caaggttttc aaagtcaccg gcgtcatcga cggcatggcc tgcaacgacg gcatgtgcgt
     4681 gaacttctac ccgcagcctg ttttttcttt ttccatcgac ggcaccgcct gcagcggcag
     4741 cacgggtgca accggttcca cgggtaccaa tggcgcaggc ggagcaaccg ggaaaaccgg
     4801 cgccaccggc ggaacgggga cagcaggaat gcccggcgca acaggaccga caggaattat
     4861 gaatcctgtt cttccgcagg atcagaacag cggcggatgc aattgcaaaa acgagataca
     4921 ggctgccatc gccggtgcga tgggcaatca ttcgggtcac ccggtggtgg acacggccgg
     4981 gtgtaaattc atgctcgtaa aaccgagtga cgcggagctg cacggcaccg acgcggccga
     5041 tacgaaatca ttctggctcg tgtttatcct cggttttctc ggcggactta ccgcgctgat
     5101 aacgccctgt gtgttcccga tgatcccgct tacggtcagc tttttcacca agcgcagcaa
     5161 agaccgcaaa acaggtttgc gcaacgcgtt cacctatgcg ctttcgatca tcgtgatcta
     5221 tgtcctgctg ggcatgctca tcacgatccc gctcggtccc gatgcgctga acgcgatggc
     5281 gagcagcgct gttttcaatc tcatcttctt tattgtcttc atcattttcg cgatgtcttt
     5341 cctgggtgcg ttcgagatca cgcttccctc cgggttcatc aaccggatcg acaaagcatc
     5401 agaccgcggc ggaatgatcg gtattttctt catggcgttc acgctctgcc tcgtttcatt
     5461 ttcctgcacg ggcccgatca tcggcacgct gctcgtggaa gccgcgcacg gcggaagcta
     5521 tcttggtcct gccattggaa tgaccgggtt cgcgctggcg ctggcgctgc cttttgcgct
     5581 gttcgcgatg ttcccgggct ggctgaactc gcttcccaaa tccggcggct ggctgaactc
     5641 ggtgaaagtg acgctcggtt ttgtcgagat cgccctggcg atgaaattcc tttccaccgt
     5701 cgatcttgcg tatcactggg gatttttcaa acgcgaaatt ttcctcggcg tgtggatcat
     5761 caccgctatg ctgctcggcc tctatcttct cggcaaactg aaactttcgc acgacagcga
     5821 catccagcat atttccaccg gccggctgat gatggcgctg ctttcattca cgttcgcact
     5881 ttacctcgtt cccgggctgt gg
//
LOCUS       DASTSF010000505         3429 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_985962,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000505 DASTSF010000000
VERSION     DASTSF010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3429
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_985962"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..362
                     /locus_tag="VFU15_14490"
     CDS             <1..362
                     /locus_tag="VFU15_14490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018285202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEU4719048.1"
                     /translation="VASDLIEHLEKEDGYRLIEMMEKLAAKKVVIFTPNGFLEQREYD
                     GNKYQVHLSGWDVEEMEKLGYKVKGINGWKPLRGEFAVIRWWPKVFWGRISLLTQKRM
                     ETRPKKAFAILCTKEIR"
     gene            complement(363..1376)
                     /locus_tag="VFU15_14495"
     CDS             complement(363..1376)
                     /locus_tag="VFU15_14495"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU4719049.1"
                     /translation="MIAVSVVIPCRNEKKYIGKCITSVLQSDFPYGEFEILVCDGKSD
                     DGTADIIRGLSAQHPNVIYVENREQVTPYALNYGAKKARGEVVIILGAHAEVDKNYLK
                     LCYEFLRDEKELGCVGGLLDNVFEDEISEAIAGAMSSRFGVGNAHFRTGTAEGYVDTV
                     AFGAYKREVFEKVGYFDEELVRNQDDEFNYRVTKAGYRIRLSKDIRAKYYVRAGFRKL
                     FRQYYQYGYWKVYVNKKHKAVTTMRQLVPPAFVAFLIGGALLSALLPFAWMIYAGLLA
                     LYLLGSFVSAIPVAKNPAGYFRTVISFWILHVSYGAGYLEGIFHFIVLGRKPSGGRQA
                     SSR"
     gene            complement(1373..2479)
                     /locus_tag="VFU15_14500"
     CDS             complement(1373..2479)
                     /locus_tag="VFU15_14500"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU4719050.1"
                     /translation="MARKILLLADADSAHTRRWALALAERGFGIGIFSLRKSVAGWAK
                     GISNIRIYDEHALSDDTFHSNVWSKLAYFRMTPKVKNAIAGFKPDVVHAHYATSYGLL
                     GRKSGFHPLVISAWGSDVMDFPSKGRLMKNMLRKNFAAADALLATSPTIVKYMNAITG
                     KKIIITPFGVDTSVFRPLEKERKREDGKIVIGLVKSLEPVYNVSVVIDTFGALLRNNP
                     GRKIGLLIVGDGTQRKMLEEKVREMHIGEQVTFAGKVDHREVPLWHNRIDIFVNISAY
                     ESFGVSVLEAMACEVPVIVSDTGGLSDIVDEGVQGFRVPVNDIAATTLAMEKLVTDAD
                     LRMRMGKAGRLKVSEKYEWENNVGEMIDVYNSLL"
     gene            complement(2479..3405)
                     /locus_tag="VFU15_14505"
     CDS             complement(2479..3405)
                     /locus_tag="VFU15_14505"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU4719051.1"
                     /translation="MSSRLSVIIVSYNVRELLSNCLVSLRKERTDFAVEIIVVDNCST
                     DGTDAMMHTDFSDVIFIGNGINRGFSGANNQGFEAATGTHFFMLNPDTEVKPGALQKL
                     MDRCVKQERLCIVGPKLLNSDGTLQRSAWKVETVWHYFLDIFYLRTIVWSGLYPEKKF
                     SREFHPEAMSGAALFFPQKLYKVLGGLDPVLFWMEDTDFCYRAEKAGAGCLFFPGAEV
                     VHHVGQSAKQNLKVTIPNQLISKLKFWRKHRSRFTVRVAKWICFIIIITRIALFTLLA
                     AANGKYRAKREAYIYALNKWYRFFYMNDNSLL"
BASE COUNT          821 a          976 c          845 g          787 t
ORIGIN      
        1 ttgttgcatc cgacctcatc gagcacctgg aaaaagaaga cggctacagg ctgatcgaaa
       61 tgatggaaaa gctcgcggca aaaaaagtgg tgatctttac gccgaacggt tttctcgagc
      121 agcgtgaata cgacggcaat aaataccagg tgcatctttc cggctgggac gtggaggaaa
      181 tggaaaagct cggctacaaa gtgaaaggca tcaacgggtg gaagccgctg cgcggcgaat
      241 ttgccgtgat ccggtggtgg ccgaaggttt tctggggaag aatttcattg cttacgcaaa
      301 agcggatgga gacgcgcccg aaaaaagcat ttgccattct ctgcacgaaa gagatccgct
      361 gatcagcggc tcgaggcctg tcttcctccc gaaggtttcc ggcccagcac gatgaaatga
      421 aaaattcctt ccaggtaacc tgcgccatag ctgacgtgca ggatccagaa tgagatgacg
      481 gtgcggaaat aacctgccgg gtttttcgcg acgggaatgg cggaaacgaa cgagccgagc
      541 aggtaaagcg caagcagccc ggcatagatc atccatgcga atggcagcag cgccgaaagg
      601 agcgcccctc caatgagaaa cgcaacgaat gccggcggaa ccagctggcg catcgttgtc
      661 accgctttgt gttttttgtt cacgtacact ttccagtagc cgtactggta atactggcgg
      721 aataatttcc ggaaaccggc acgcacataa tatttcgcgc ggatatcttt cgaaagacgt
      781 atccggtagc cggccttcgt cacgcggtaa ttgaattcat catcctggtt gcgcaccagt
      841 tcctcgtcga aataaccgac tttctcaaac acttcccgct tgtaagcacc gaaggcaacc
      901 gtgtctacat aaccttccgc cgttcccgtc cggaaatgcg cattgcccac cccgaaacgc
      961 gaggacatcg ccccggcgat cgcttccgaa atctcatctt cgaaaacatt gtcgagcaaa
     1021 ccgccgacgc agccgagctc tttttcatcc cgcaaaaatt cgtaacagag tttcaggtaa
     1081 tttttatcca cctcggcatg cgccccgaga atgatgacaa cctctcccct cgcctttttc
     1141 gcgccataat tgagcgcgta cggcgtcacc tgctcgcggt tctcgacgta aattacattc
     1201 ggatgctgcg cagaaagccc gcggatgatg tccgctgttc cgtcatcgct tttcccgtca
     1261 cagacgagaa tttcaaattc cccgtatgga aaatccgact gcagcacgga ggtgatgcat
     1321 ttcccgatgt attttttttc attcctgcac ggaatgacga ctgaaacggc gatcataaaa
     1381 ggctgttata aacatctatc atttcaccga cattattttc ccattcatat ttttcagaga
     1441 ctttcaggcg accggctttt cccatgcgca tgcgaagatc cgcatccgtc accagcttct
     1501 ccatcgcgag tgtcgtcgca gcaatatcat tcaccggaac gcggaatccc tggacgcctt
     1561 cgtcgacaat atccgaaaga ccgccggtat ccgaaacaat cacgggaact tcgcaggcca
     1621 tggcttcgag cacggagacg ccgaagctct cgtacgcaga aatgttcacg aagatgtcga
     1681 tgcggttgtg ccataacggg acttcgcggt gatcgacctt cccggcaaat gtcacctgtt
     1741 ctccaatgtg catttcacgc actttttctt ccagcatttt ccgctgcgtc ccgtcgccga
     1801 caataagcaa cccgatcttt cttcccggat tattccgcag aagcgcgccg aacgtgtcga
     1861 taacgacgct cacgttgtac accggttcca gcgattttac caggccgatc acgatctttc
     1921 catcttcccg tttccgttcc ttttcgagcg gccggaacac ggacgtgtcg acaccgaaag
     1981 gcgtaatgat gatcttttta ccggtgatcg cgttcatgta tttcacgatg gtcgggctgg
     2041 tcgcgagcag cgcatccgcc gccgcaaaat ttttgcggag catgttcttc atcagccttc
     2101 ctttcgacgg gaaatccatc acgtcgcttc cccacgcgga aatgaccagc gggtggaaac
     2161 cgctcttcct cccgagcagg ccgtagctgg tggcgtaatg cgcgtgcacg acatccggtt
     2221 tgaaaccggc tatcgcattt ttcactttcg gcgtcatccg aaaataagcc agcttgctcc
     2281 agacgttgct gtgaaacgta tcgtcgctta acgcgtgctc atcgtaaatg cggatgttgc
     2341 tgattccctt tgcccaaccg gcaacgcttt tccgcaggct gaaaatacca atgccgaatc
     2401 cccgttccgc gagcgcaagc gcccacctgc gcgtgtgcgc agagtcagca tcggcgagca
     2461 ggaggatttt gcgggccatc acaaaaggga attatcgttc atgtaaaaaa agcggtacca
     2521 tttattcagc gcataaatgt acgcctcccg tttcgcccgg tattttccat tggctgcagc
     2581 gagcaacgta aaaagcgcaa tgcgcgtaat gatgatgatg aaacaaatcc atttcgccac
     2641 acggacggta aagcggctcc tgtgctttcg ccagaacttc agcttgctga tgagctggtt
     2701 cgggatcgtc actttcaggt tttgcttcgc gctctgcccg acgtgatgca ccacttccgc
     2761 acccgggaaa aacaggcagc cggcgccggc cttttccgcg cggtagcaaa aatccgtatc
     2821 ctccatccag aaaagcaccg ggtcgagtcc gccaagaact ttgtacagct tctgcgggaa
     2881 gaagagcgcg gcgcccgaca tggcttcggg atgaaattcc cgggaaaatt tcttctccgg
     2941 gtacaacccg ctccatacga tcgtgcgcag gtaaaaaata tcgaggaaat aatgccacac
     3001 cgtttctact ttccatgccg aacgctgcaa cgttccgtcg gaattcagga gtttgggtcc
     3061 gacaatgcag agacgctcct gcttcacaca acgatccatc agcttctgca gcgcgccggg
     3121 tttcacttcg gtatcgggat tgagcataaa aaaatgcgtt cccgtagccg cttcaaaacc
     3181 ctggttattg gcgccggaaa atccccggtt gatcccgttc ccgataaaga tgacatcgct
     3241 gaaatccgta tgcatcatgg catccgtgcc gtccgtagaa cagttatcca ccacgatgat
     3301 ctccacagca aaatccgtcc gttccttccg cagcgaaaca aggcagttgc tgagaagctc
     3361 ccgcacgtta taactgacaa ttatgacgga aagcctgctg ctcattcgga cagagatgta
     3421 tgttcattt
//
LOCUS       DASTSF010000506         7458 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1487346,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000506 DASTSF010000000
VERSION     DASTSF010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 7458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7458
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1487346"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..413)
                     /locus_tag="VFU15_14510"
     CDS             complement(<1..413)
                     /locus_tag="VFU15_14510"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HEU4719052.1"
                     /translation="MKRLFHFAPLLFLLFAVTGAGAQSIAGFSRVDSIAVYDGGQLRN
                     PWSGGHNYCQLSEIDLNGDGIKDLFVFDRSGNKITTYINLGTPNQVDYVLAPEYVSRF
                     PLMHDWCLLRDYNCDGKEDIFTCSIAGFAIYKNVST"
     gene            599..3703
                     /gene="secDF"
                     /locus_tag="VFU15_14515"
     CDS             599..3703
                     /gene="secDF"
                     /locus_tag="VFU15_14515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010526433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecDF"
                     /protein_id="HEU4719053.1"
                     /translation="MQNIKGAIRIFAILMAASCLFYLSFTFVTHSWEKKAHQYAENYI
                     TNPSIVKMAEDRSGGDPVLKAKILDSVLTARERFFLEDSIAGKKIYLKLYTYESCKDK
                     ELNLGLDLKGGMNVTLEVSTPDIVKALSNYSTDPGFTQAIEKAQAAQANSNKDFVTLF
                     GEAYHEVNPQGRLSSLFASQLKQYDITYNSTDDQVLTAIRKEADAAVDRAKLVLETRI
                     NKFGVSQPNIQHLQGTNRILVELPGVKDEERVGKLIQASANLEFWETYEVNDIAQKVN
                     DANEKLKVLLGYKSDTLHADSAKLRADYSAAKTKKDSLKADSMAKVAFNNDVKRVDSL
                     KKANDSTTKKANAAAGKKEAQPLFDHIVAGNPYNNKGPIVFGALIKDTSVIGELLRRP
                     EIAPIFPRDLHFAWTVKPEYTQTGQPFLWLIALKGSKQNNGAALPGDVVTEARKVFDR
                     MNGTGSPEIQMTMNAEGARNWARLTKANVGHSVAIVLDNYVYSFPNVQNEITGGISSI
                     TGSFSNKEADDLVNVLNTGKLPAPTNIVEKTVVGATLGQEAIRSGILSFIVALIVVLI
                     YMGFYYNKAGWVADLALFVNVFFIMGILSSLGAVLTLPGIAGIVLTIALSVDANILIF
                     ERVREELREGKSLSNAIADGYKHAMSSILDSNLTTLILGIILYSFGSGPVQGFATTLI
                     IGIISSLFCAIFITRLVFDWMLKKEQKVTFSTPRTENVLRNAKINFVGKRKYYYMFSG
                     TIIALGVIFYFKHNGFTKGVDFSGGRTYTVRFEKAVDREAVRNALKVEYNEPPEVKTA
                     GGNDQLKITTNYRVSEVSTDVDKDVMQKLNDGLKHAVGDMKYEIVSSVKVGPSIANEV
                     INKSFIVIIISCLLMFLYILFRFRKWQYGLGAVAALVHDVLVVLSCYTIFDGILPFPL
                     EVDQHFVAAILTVMGYSMTDTVVVFDRIREFLANQGKDLATAEKTSTINYALNSTLSR
                     TINTSMITFFVLLSIFIFGGETIRGFSFALLIGIVIGTYSSLCIATPIVVDFDRRKDR
                     DEQK"
     gene            3794..4192
                     /locus_tag="VFU15_14520"
     CDS             3794..4192
                     /locus_tag="VFU15_14520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEU4719054.1"
                     /translation="MIHVPLIVLFLNLGSGEVFFIILIMLFFFGADKLPAMARALGKG
                     MREMKNATAEIQREIEKSAGEVQEGMNLNDTLKDLREATDNLKNHLKEGVSNLENHYS
                     NALKDDKKDAPPEEEKKTDDPISDDGSIKR"
     gene            4361..6550
                     /locus_tag="VFU15_14525"
     CDS             4361..6550
                     /locus_tag="VFU15_14525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015025155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HEU4719055.1"
                     /translation="MSQIRFKALEQTMHRTPVNVETPAGGVASYFGENVFGIDAMRQF
                     LSEEALASVLGTIERGERIDRKMADQIASGMKAWATSKGATHYTHWFQPLTGATAEKH
                     DAFFEPIEGGRAIERFGGSQLVQQEPDASSFPSGGIRNTFEARGYTAWDCSSPAFIIG
                     DTLCIPTIFVSYTGEALDYKTPLLKALYALDKAAVDVCQYFDKNVTKVIATLGWEQEY
                     FLVDESLYNARPDLAISGRTLFGASPAKGQQLEDHYFGSIPDRATTFMRDLEIEAHKL
                     GIPIKTRHNEVAPNQFECAPIFEEINLAVDHNQLLMDIMEKTARRHSFRVLLHEKPFA
                     GVNGSGKHNNWSMATNTGKNLLSPGKTPKTNLQFLTFFINTIKAFHDHADLLRAAIAS
                     ASNDHRLGANEAPPAIMSVFLGSQLSAILDELEAKVKSGKMTPDEKTGLKLNIGKIPD
                     ILLDNTDRNRTSPFAFTGNKFEFRAVGSSANCAGAMTVLNTIMAEQLIKFRHDVDALM
                     AKNVEKDEAIFQVLRQYIVASKKVRFEGNGYSDEWVKEAKKRGLNNFKTTPEALEAFN
                     SKHAHELFARHNVLSEREQHARYEIHLETYVKKIQIEARVMGDMAVNNIIPTALQYQN
                     TLISNVRGLKEVMGAAEFKKAAAVQLEMIAEISEHVSGIKKNVDDMIEERKKANNIED
                     MKKKADAYCNKVKPYFDVIREHVDKLEMIVADELWPLPKYREMLFTK"
     gene            6737..>7458
                     /locus_tag="VFU15_14530"
     CDS             6737..>7458
                     /locus_tag="VFU15_14530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU4719056.1"
                     /translation="MKRIILLSSAIAFSVFSYAQQAIRKADYKYSCNAYYNAQQYYSG
                     AAAKAGSKAQKAYAYFQAGECARMNNDTKAAITNYGKAIKAKTDRDDVYLRLAQCQMM
                     KGDYLHALENFTEYKKRVPSDPAADTGIVSCSLAPQWMIDSTRWKVTNVKELNTEYSD
                     FCPAWFDKKHNALMFTSKRPGQTGSKIDPNTGNLYSDMFQATESKTGHWNTPQTVAGD
                     VNLPMANDGACSVTKNGSHIFFT"
BASE COUNT         1964 a         1966 c         1923 g         1605 t
ORIGIN      
        1 gtggaaacat ttttgtatat cgcaaacccg gcaatggagc aggtaaaaat atcttccttg
       61 ccgtcgcagt tgtaatcacg cagcaggcac cagtcgtgca taagcgggaa ccggctcacg
      121 tattcaggag cgagcacgta atccacctgg ttcggtgtgc cgaggttaat ataggtggtg
      181 atcttgtttc ccgacctgtc gaatacgaac agatccttga tgccgtcgcc gttaagatcg
      241 atctcggaaa gctggcagta attatgtccg cccgaccacg ggttgcgcag ctgtccgcca
      301 tcgtacaccg ctatggaatc gacgcgggag aacccggcaa tggactgtgc accggcaccc
      361 gttaccgcga aaagaagaaa aagaagcggt gcgaagtgga aaagtctttt catgccaact
      421 gatttttatg agacttccgg acgccggcgg ggagataaac aaagataagc caaaatcgat
      481 cctgccggac tggttttgaa ttgggtagtt aggtttttct tctatatttg caccccacaa
      541 tttgggaacg gtacggctgg ttgagtcagg cttttccccg gttaagtgat taatacctat
      601 gcaaaacatt aaaggagcaa tccggatttt cgccattctg atggcggcga gctgtctgtt
      661 ttacctctcg ttcacgttcg taacccacag ctgggagaaa aaagcccacc agtacgcgga
      721 gaactacatt actaacccgt ccattgttaa aatggcagag gatcgttcag gcggcgatcc
      781 cgttcttaaa gcgaagatcc tcgattcggt gctaaccgcg cgcgaacgtt ttttcctcga
      841 agattccatc gcaggaaaga agatctacct gaagctgtac acctacgaaa gctgtaagga
      901 taaggagctc aacctcggcc tcgatctcaa gggcggaatg aacgttacgc tggaggtttc
      961 caccccggat atcgtcaagg ccctgtccaa ttattccact gatccgggct ttacgcaggc
     1021 cattgaaaaa gcgcaggccg cgcaggcgaa ttccaataag gatttcgtaa cgctcttcgg
     1081 tgaagcgtat catgaagtga atccgcaggg acgtctttcc agtctcttcg cttcccagct
     1141 caagcaatac gacatcacgt ataattccac ggatgaccag gtgctcactg ctatccgcaa
     1201 ggaggcagat gcggccgtcg atcgtgcgaa gctcgtgctg gaaacacgta tcaacaaatt
     1261 cggcgtgtca cagccgaata tccagcatct ccagggaacc aaccgtatcc tcgtggagct
     1321 tccgggtgtg aaggatgaag aacgcgtagg aaagcttatc caggcttccg cgaatctcga
     1381 attctgggaa acctacgagg tgaatgacat cgcgcagaaa gtaaatgacg cgaacgaaaa
     1441 gctgaaagtg ctgctcgggt acaaatcaga tacgctgcac gcggattccg ccaagctcag
     1501 ggctgattat tcggcggcaa agaccaaaaa agattccctc aaagccgaca gcatggcgaa
     1561 agtcgcgttc aataatgatg tgaaacgcgt cgacagcctc aagaaagcga atgattccac
     1621 cacgaaaaaa gcgaatgccg ctgccggcaa gaaagaagcg cagcccctgt tcgatcatat
     1681 cgtcgcaggg aacccgtata ataataaagg accgatcgtc ttcggtgcgc tgatcaagga
     1741 tacgtccgtc atcggcgagc tgctgcgccg tcccgaaatc gctcccattt tcccgcgcga
     1801 tcttcatttt gcgtggaccg taaaaccgga atatacgcaa acgggacagc ctttcctctg
     1861 gctcatcgca ttgaagggca gcaagcagaa caacggcgcg gctctcccgg gtgatgttgt
     1921 caccgaagcc cgcaaggtgt tcgaccgcat gaacggaacc ggcagccccg agatccagat
     1981 gaccatgaac gccgaaggcg cccgcaactg ggcccgcctg acgaaggcga acgtcggcca
     2041 cagcgtggcg attgtgctcg ataattacgt gtattctttc ccgaatgttc agaacgagat
     2101 cacaggaggc atttcctcta tcaccggatc tttctcgaac aaagaagcgg atgacctcgt
     2161 gaacgttctt aacaccggca agcttcccgc tcccacgaac atcgtcgaga aaacggtggt
     2221 cggagctacg ctcggacagg aagcgatccg ttccggtatc ctttcgttta tcgtcgcgct
     2281 gatcgtggtg ctcatttaca tgggattcta ttacaacaag gccggctggg tggctgacct
     2341 tgccctgttc gtgaacgtct tcttcataat ggggatcctc agctcgcttg gcgcggtgct
     2401 cacgcttccc ggtatcgcag gtatcgttct cacgatcgct ctttccgtcg atgcgaacat
     2461 ccttattttc gaacgtgtcc gtgaagaatt acgcgaagga aaaagtcttt cgaacgccat
     2521 cgccgacggc tacaagcacg cgatgtcttc gatcctcgac tccaacctga cgacacttat
     2581 tctcggtatc atcctgtatt ccttcggtag cggcccggta cagggttttg cgaccacgct
     2641 gatcatcggt atcatcagct cgctcttctg cgcgatcttc atcacgcgtc ttgttttcga
     2701 ctggatgctg aagaaagaac agaaagtaac gttctccacc ccgagaacag aaaatgtgct
     2761 gcgtaacgcg aagatcaact tcgtcgggaa gcggaagtat tattatatgt tctccggaac
     2821 gatcatcgct ctcggtgtga tcttctactt caagcacaac ggtttcacca aaggcgtgga
     2881 tttcagcggt ggccgtacat atacggtccg cttcgagaaa gcggtcgacc gtgaagccgt
     2941 gcgcaacgcc ctgaaagtcg aatacaatga accgccggaa gtgaaaacag caggcggaaa
     3001 cgatcagctc aagatcacga cgaactaccg cgtcagcgaa gtgtcgacag atgtcgacaa
     3061 ggatgtgatg caaaagctga atgacgggct caaacatgct gtcggcgata tgaaatacga
     3121 gatcgtcagc tccgtgaaag tgggtccgtc catcgcgaac gaagtcatca ataaatcgtt
     3181 catcgttatc atcatctcct gcctgctgat gttcctgtac atccttttcc ggttccggaa
     3241 atggcagtac ggacttggtg ctgtcgcggc gctcgtgcac gacgttctcg tcgtcctttc
     3301 ctgctacacc attttcgacg gcatcctgcc tttcccgctg gaggtcgatc agcatttcgt
     3361 agccgctatt cttacggtga tgggttattc catgacagat accgtcgtgg tgttcgacag
     3421 gatccgcgaa ttcctcgcca accagggcaa ggatctcgct accgctgaaa aaacaagcac
     3481 catcaactac gcgctcaaca gtacgctgtc gcggacgatc aacacatcca tgatcacgtt
     3541 cttcgttctt ctttccatct tcattttcgg tggcgagacg atccgcgggt tctccttcgc
     3601 gctgcttatc ggtatcgtga tcggaaccta ttcctctctc tgcatcgcaa cgcctatcgt
     3661 ggtcgacttc gaccgccgca aagacaggga cgagcagaaa taaaaacacg attgatcaac
     3721 agtatcgcag ccccggtttt aacacggggc tgctttgctt tacgggaaaa caaataccta
     3781 actttgttcc ataatgatcc atgtgccgct gatcgttttg tttctcaacc tgggctccgg
     3841 agaagtattc ttcatcatcc tgatcatgct tttctttttc ggtgcggata agttgcccgc
     3901 catggcccgt gcgctcggga aagggatgag ggaaatgaaa aacgctacgg ctgaaattca
     3961 gcgggaaata gaaaaaagcg caggtgaagt gcaggaagga atgaacctta acgatacgct
     4021 caaggatttg cgtgaagcca ccgataatct gaaaaaccac ctgaaggaag gcgtcagcaa
     4081 cctcgaaaat cattacagca atgcgctgaa ggatgacaag aaagacgctc ctccggaaga
     4141 agagaaaaaa acagacgacc cgatcagcga tgacggttcg ataaaaagat aatgcaggcc
     4201 cggtagggcc ttttttttag gggtataacc ggaaaaagtg atgtttttgg ggtagtaccc
     4261 caatttttta gggggtgttg ataaaaaaag aactgttttt tcagacacac cccctgtttt
     4321 gggatacatt tgcgccgtcc aacgcaaaaa aacagcagcc atgtcccaaa tccgcttcaa
     4381 agcactcgag caaaccatgc accgtacgcc ggtgaatgtg gaaacacccg caggaggtgt
     4441 cgcctcttat ttcggagaaa atgttttcgg tatcgacgcc atgcgccagt tcctttcgga
     4501 agaagcgctg gccagcgttc tcggcaccat tgaacgcggc gaacgcatcg accgcaagat
     4561 ggcagaccag atcgcgtccg ggatgaaagc ctgggcgacc agcaaaggtg ccacgcatta
     4621 cacgcactgg ttccagccgc ttaccggcgc aacagccgag aagcatgatg cttttttcga
     4681 accgatcgaa ggcggaagag ccattgagcg tttcggcgga tcgcagctcg tacagcagga
     4741 gcccgacgca tccagcttcc ccagcggagg aatacggaat acgttcgaag cgcgcggtta
     4801 cacggcatgg gattgctcct cgcccgcatt tatcatcggc gatacgctct gcattcccac
     4861 gatcttcgtt tcttatacgg gcgaagcgct cgattataaa acgccgctgc tgaaagcgct
     4921 ctatgcgctc gataaagctg cagtggatgt gtgccagtat ttcgacaaga acgttacgaa
     4981 agtgatcgcg acactcggct gggagcagga atattttctc gtggatgaat cgctgtacaa
     5041 tgcacgcccc gacctggcca tttccggccg gacgcttttc ggcgcaagcc cggcgaaagg
     5101 acagcagctg gaagatcatt atttcggttc cattcccgat cgcgccacga cgttcatgcg
     5161 cgacctcgag atcgaagcgc acaagctcgg catcccgatc aaaacgcgcc acaacgaagt
     5221 tgcgccgaac cagttcgaat gcgcacccat tttcgaagag atcaacctcg ccgtcgatca
     5281 caaccagctg ctcatggaca tcatggaaaa aacggcacgc cgccacagtt tccgcgtgct
     5341 gcttcatgaa aaaccattcg caggtgtgaa cggttccggc aagcacaaca actggtcgat
     5401 ggcgacgaat acgggaaaaa atctgctgag cccggggaaa acgccgaaaa ccaacctgca
     5461 attccttacg ttcttcatca acaccatcaa agcttttcat gatcacgccg acctgctgcg
     5521 cgcggccatc gcttccgcca gcaacgacca ccgtctcggt gcgaacgaag ctcctcctgc
     5581 gatcatgtcc gtgttcctcg gctcgcagct ttccgctatt ctcgatgaac ttgaggcaaa
     5641 agtgaaaagc gggaaaatga cgcctgatga aaaaaccggg ctgaagctca acatcggcaa
     5701 gatccccgat attctcctcg acaacaccga ccgcaaccgt acgtctccgt ttgccttcac
     5761 gggaaataaa ttcgagttcc gcgcagtggg ctcttccgcg aattgcgccg gcgccatgac
     5821 cgtgctgaat acgataatgg ccgagcagct gataaaattc aggcacgacg tggatgcgct
     5881 tatggcgaaa aacgtcgaga aagacgaggc gatcttccag gtgctgcgcc agtacatcgt
     5941 tgcatcgaag aaggtgcgtt tcgaaggaaa cggttacagc gacgaatggg tgaaggaggc
     6001 gaagaagcgc ggactgaaca atttcaaaac cacacccgaa gcgctggaag cgttcaactc
     6061 aaaacatgcg cacgagctct ttgcgcgcca caacgtgctc agcgaacgcg aacagcatgc
     6121 gcggtatgaa attcatcttg aaacgtacgt gaagaaaatt cagatcgagg cgcgtgtgat
     6181 gggagatatg gccgtgaata atattattcc tacggcgctg caataccaga atacgctgat
     6241 cagcaatgtg cgcggactga aagaagtgat gggtgccgcc gaattcaaaa aggccgccgc
     6301 tgttcagctg gagatgatcg cggaaatttc ggagcatgta agcggcatca agaaaaatgt
     6361 cgacgacatg atcgaagagc ggaaaaaagc gaacaacatc gaggacatga aaaagaaagc
     6421 ggacgcttac tgcaataagg ttaaacctta tttcgacgtg atccgcgaac atgtcgataa
     6481 gctcgaaatg atcgtggccg acgaattgtg gccgctgccg aaataccgcg agatgctttt
     6541 cacgaaataa acgtgaagga aattttgaaa ggaccggtgg aagaccggtc tttttttttg
     6601 agctcgtgca cggattttcc ggaatgctcc gtgtatcttt ttttcccgcg caggttataa
     6661 ccgcaaagac aggataaagg ctttggccgg ccactttcct tcatcgctgt ccgacgatca
     6721 acaaaaactc ggacacatga aacgcataat cttactcagc tcagcaattg ctttttccgt
     6781 tttcagttat gcacagcagg cgatacggaa agcagattac aaatattcgt gcaatgcgta
     6841 ttataacgca cagcagtatt acagcggtgc cgctgcaaaa gcaggaagca aagcgcagaa
     6901 agcgtacgct tatttccagg cgggcgaatg cgcgcggatg aacaacgaca cgaaagccgc
     6961 catcaccaat tacgggaaag ccatcaaagc gaaaaccgat cgcgatgatg tttatttgcg
     7021 gctcgcgcaa tgccagatga tgaaaggcga ttatctgcac gcgctcgaaa atttcacgga
     7081 atacaaaaaa cgcgtgccct ccgacccggc ggcggatacg ggaattgttt cctgttcgct
     7141 cgcgccgcaa tggatgatcg attctacacg gtggaaagta acgaacgtga aagagctcaa
     7201 cacggaatac agcgatttct gccctgcctg gttcgataaa aagcacaacg cacttatgtt
     7261 cacatcgaaa cggccgggac agacgggaag caagatcgat ccgaacaccg ggaaccttta
     7321 cagcgatatg ttccaggcga ccgaaagcaa aacgggacac tggaacacgc cgcagacggt
     7381 tgcaggtgat gtgaacctgc cgatggcgaa cgacggtgcg tgcagcgtaa cgaagaacgg
     7441 ctcgcacatt tttttcac
//
LOCUS       DASTSF010000507         3509 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_813630,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000507 DASTSF010000000
VERSION     DASTSF010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3509
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_813630"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1867)
                     /locus_tag="VFU15_14535"
     CDS             complement(<1..1867)
                     /locus_tag="VFU15_14535"
                     /inference="COORDINATES: protein motif:HMM:NF017393.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M43 family zinc metalloprotease"
                     /protein_id="HEU4719057.1"
                     /translation="MKKRLLFILGGSLAFGASFSQNLINASLPASSAYTASTSTSAYY
                     DVSGKSMIRFNTDIITQQQMDQVNQDRHDFLVAENPNYDADRSAYEANIQQYIAAHGS
                     QVTSQSVYTIPVVVHVVYNTTTQNISDNQVFSQIQVLNEDFGRTNADANTHWSQAANT
                     TIQFCLAQQDPNGNATNGIERRHITTNVSFGTNDNVKHFSSNGLDAWDPTRYLNIWVC
                     NLGSSLLGYGEFPTTSVSQTFGVVILYSAFGSNFTSYGTFADIQSPYDRGRTTTHEFS
                     HCFNLYHIWGDDNGACTGTDYCADTPNQANATTTCYTFPHTDACTGSSPGIMFENYMD
                     YSYDNCLNLFTNNQKSRMLAVLSTAPYNALTTSNGCQAPSASVAPVAAFTMSASSVCA
                     GTAVNFTDNSTNSPTSWSWTFPGGSPGTSTAQNPAGITWSAAGTYTVSLTASNGSGSN
                     TITHTITILALPSVTTSTSGATICSGSSTTITASGASTYSWMPGSLSGTTVTVSPATT
                     TTYTVTGTNANGCTNTATRTITVNASPTVTTTASSSTVCSGTGVTLGASGASTYNWMP
                     GNHNGQPWTTTPTSTTTYTVTGTSANGCTGTATRTITVNASPTVTTTASPSTICSS"
     gene            complement(2214..>3509)
                     /locus_tag="VFU15_14540"
     CDS             complement(2214..>3509)
                     /locus_tag="VFU15_14540"
                     /inference="COORDINATES: protein motif:HMM:NF039201.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEU4719058.1"
                     /translation="TGSGASTYTWQPGNLSGASVSDNPASGTTYTVTGTDANGCVNTS
                     TVSVAVNPVPTVSASATPGTICAGSNITLTGSGASTYNWMPGNISGSPATDTPLSTTT
                     YTVSGTDVNGCSGTATVAVTVNPAPNVSASASASGICLGSSVNLTASGATSYLWQPVN
                     QSGSAITDTPSATTTYTVTGTDATGCSGSDTVSVSVYLQPTITISGTSPICPGNSVTL
                     TANGGTSYLWQPGNQTTSSITDTPSSATTYSVTGTDISGCSNTATFTVNIDTPPSTPS
                     IIVNGATLTSSVTGSSYQWFLNGNPIAGATGQSYTATQTGSYTVEVYDTAGCGSGQSS
                     AVVDPTGIAGTTTDNSGLSVTPNPNDGHFVLTFSSAKPENYVIEIHNMLGQVVYSEKL
                     DNFSGDYHKDIDLSVYGRGVYSIRLRSENHETVIKTITY"
BASE COUNT          736 a          828 c         1048 g          897 t
ORIGIN      
        1 cggaagaaca gatcgttgac ggcgatgctg ttgtcgtcac ggtcggcgaa gcgtttaccg
       61 tgatcgttct tgtggcagta ccggtacatc cgttggcgct tgtgccggtt acagtgtatg
      121 ttgtggttga ggtgggcgtg gttgtccacg gctgcccgtt gtgatttccc ggcatccagt
      181 tgtaagtcga agcgccggac gcccccagcg ttacgcctgt tccggaacaa actgtcgaag
      241 aagaagccgt tgttgtcacg gtcggcgaag cgttcaccgt gatggttctt gtcgctgtgt
      301 tggtacatcc gttcgcgttt gttcccgtaa cggtatacgt ggttgtcgtt gccggcgaaa
      361 cagtaaccgt cgttccggaa aggcttcccg gcatccagga ataggttgag gctccgctgg
      421 cggttatggt cgtgctgctt ccggaacaga tcgttgctcc gcttgtgctg gtggttacgc
      481 ttggcaatgc aaggatcgtg atcgtatgtg tgatggtatt gctgccgctt ccgttgcttg
      541 ccgtaagcga aaccgtgtat gtgcctgcag cgctccaggt aattcccgcc gggttctgtg
      601 ccgtggatgt tcccggcgat ccgccgggga atgtccagct ccagctggtc gggctgttgg
      661 tggaattatc cgtgaaattc accgccgttc ctgcgcagac agaacttgcg ctcatggtaa
      721 atgccgcaac cggagcaaca cttgcagacg gagcctggca gccattggag gttgtaagcg
      781 cgttatacgg tgcagtggaa agcactgcca gcatgcgcga tttctggttg tttgtgaaaa
      841 ggttaaggca gttgtcgtaa ctgtaatcca tataattctc gaacatgatg cccgggctgg
      901 agccggtgca tgcgtccgta tgggggaacg tatagcacgt ggttgtcgcg ttggcctggt
      961 tgggcgtatc ggcgcagtaa tcggttcctg tgcacgctcc gttatcatct ccccagatgt
     1021 gataaaggtt aaaacaatgt gagaactcat gtgttgtggt gcgcccgcgg tcatacggcg
     1081 actggatgtc ggcaaacgtt ccgtaggaag tgaagttgct tccgaaggcg gaatagagaa
     1141 tcaccacacc gaaagtttgc gaaacagatg ttgtcggaaa ttcgccgtag ccgagaaggc
     1201 tcgagccgag gttgcacacc cagatgttga ggtaacgcgt cggatcccat gcatcaaggc
     1261 cattcgagct gaaatgtttt acgttatcgt tcgttccgaa agaaacattc gtggtgatgt
     1321 gcctgcgctc gatcccgttc gtggcattgc cgttcgggtc ctgctgcgca agacagaatt
     1381 gtatggttgt attcgccgcc tgcgaccaat gtgtatttgc atcggcattc gttctgccga
     1441 aatcctcgtt cagaacctgg atctgggaaa agacctggtt gtcggaaata ttctgcgtgg
     1501 tcgtattata aaccacatgt acgacaactg ggatcgtgta aacggactgc gaggtaacct
     1561 gtgaaccgtg cgccgcgatg tattgctgga tattggcttc gtatgcgctg cggtcggcgt
     1621 cgtaattcgg gttttccgcc accaggaaat cgtgcctgtc ctggttcacc tggtccatct
     1681 gctgctgagt aatgatatcc gtgttgaagc ggatcattga ttttccgctt acgtcgtaat
     1741 aggccgaagt cgatgtgctg gccgtataag cagaagaggc aggaagagag gcattgatca
     1801 ggttctggga aaaagatgcg ccgaacgcaa ggcttccgcc caaaatgaaa agcagtcttt
     1861 ttttcatgca aagatggttt tgtcaggtat gcgttttaat aacgagttct cttttttgaa
     1921 aggagaagta atttgaaata ataatactta ctgattggct gcacttaaat ctgtagggat
     1981 gtgagagggg aaatttccac acccggtcaa taaagaacac gttgtttatc gtaaccggcg
     2041 ggcagcaatt tttcgcaacc gggaaagcaa gtatacgcat gaaacgggtc agtgtcaata
     2101 ctatccgcgc ttatttgttt tgcggtgtag ttcaaggcgg cctgatttca gtatcctgac
     2161 gggaacaaaa aaaggccgtc actgacggcc tttaaaggat tagcccgaaa tgatcagtac
     2221 gtgatcgttt tgatgaccgt ttcgtgattt tcactgcgga gacgaatgct ataaacgccg
     2281 cggccataca cggaaaggtc aatgtctttg tggtagtcgc cggagaaatt atcgagcttt
     2341 tcagaataca cgacctgtcc aagcatgttg tgaatttcaa tcacatagtt ttccggtttg
     2401 gcgctcgaga aagtcagcac aaagtgaccg tcgttcgggt tgggcgtcac agaaagcccg
     2461 gaattgtcgg tcgtcgttcc tgcgattccc gtgggatcca caacagcgga agactgccct
     2521 gatccgcatc cggcagtgtc ataaacttca accgtatacg acccggtttg tgttgcggtg
     2581 tacgattggc cggttgcacc cgcgatcggg tttccgttca ggaaccattg ataggatgag
     2641 cctgtgacgc tcgaggtcag cgttgcgcca ttcacaatga tcgacggagt cgaaggcggc
     2701 gtatcgatgt tgacggtaaa tgttgccgtg ttggaacagc cggaaatgtc ggttccggtc
     2761 accgaataag tggtcgcgct ggaaggcgtg tcggtgatgc tggatgttgt ctggtttccg
     2821 ggttgccaca aataagatgt tcccccattc gccgtaagcg ttacgctgtt gcccgggcag
     2881 atcgggctgg tgccggaaat ggtgatcgtc ggctggagat aaaccgaaac agagacggtg
     2941 tcgctgcccg agcatcccgt agcgtctgtt cccgttactg tatacgttgt cgttgccgac
     3001 ggcgtatcgg ttattgcgga gccgctttgg ttcacgggtt gccacaggta agacgtagcg
     3061 cccgacgctg taagattcac ggagcttccg agacagattc cgctcgctga agcggaagca
     3121 gaaacattcg gtgcgggatt taccgtaaca gcgactgttg ccgtacctga gcaaccgttc
     3181 acatcggttc cgcttacggt gtaagtcgtt gtggaaagcg gagtgtctgt agcaggcgat
     3241 ccgctgatat ttccgggcat ccagttgtag gttgacgctc cgctgccggt aagtgtaata
     3301 ttacttcctg cgcagattgt tcctggtgtt gcggaagcgg aaaccgtcgg caccgggttg
     3361 acagcgacac tgacagtgga tgtattcacg cagccattgg catccgttcc ggtcacggtg
     3421 taggtagtgc ccgacgccgg gttatcgctg acggacgcgc cgctgaggtt tccgggttgc
     3481 cacgtgtagg tggacgcgcc gcttcccgt
//
LOCUS       DASTSF010000508         2520 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_972395,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000508 DASTSF010000000
VERSION     DASTSF010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2520
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_972395"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..715
                     /locus_tag="VFU15_14545"
     CDS             <1..715
                     /locus_tag="VFU15_14545"
                     /inference="COORDINATES: protein motif:HMM:NF023179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HEU4719059.1"
                     /translation="HLYLGEHNRLGAGIQAGYAQRSMNYSAFQWGNQYDVNAFNPNLP
                     SGEPSAGAAFGYADFGTGIVWTYENASRSDQVANNHELKINLGAGFFHVNQPAYSFYK
                     TPGEKLYMRTSIHGSAIIGIPYSSFSLVPGFMYSMQGTAREIYAGTMIRYTTVRTDVK
                     YGNLQGTALSLGAYLRTKDAVAAVMMLEYSSFAAGFSYDFNVSTLKAASSRQGGFEIA
                     LRYVFPDPFSFRSSGQSFY"
     gene            907..>2520
                     /locus_tag="VFU15_14550"
     CDS             907..>2520
                     /locus_tag="VFU15_14550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin domain-containing protein"
                     /protein_id="HEU4719060.1"
                     /translation="MIRRITFLSPALFALLNFTNNASAQMFTPGNIVVEQVGDGTSAL
                     ASGVAAPLFLVEYSATGTLTNSVALPVAGPNVITTSGTATSEGNISLSANGRYIIVPG
                     YNALPGTTGLTSTTSATVARVTGQVDASGTYSMPSVTNTYFSGNNIRGAASDGNNNYW
                     ATGANTGACYFGTGTPAIVYSTNTNNRAMAVYNGNLMFSAGAGTRGIYQFTGMPTTAS
                     AASLILPLTGSPYQFAINSTSTILYVADDASFTSTSSQAGGIEKFTWNGSAWVFQYTL
                     QCGNASTAGARGLVVDFSGTNPVVYASTAESSSNRIISITDAGASSGSSATLLATAAT
                     NTIFRGLAFSPCSPPSITTVSSNSPVCSGSTLNLSSAVTGSAPLTYSWTGPNSFSGNT
                     ANPSISSVTTAAAGTYMLNLSNACGSASQNTTVVVNTPPSITTITNTSPVCSGSDINF
                     NSAASGSAPLTFSWTGPNSFSSNSATPSITGATPLANGIYTLTVTNGCGSDMQTTVAF
                     ASLPVASTTSQTDVTCNGANDGTASVTVSG"
BASE COUNT          595 a          774 c          599 g          552 t
ORIGIN      
        1 ccacctgtac ctcggtgagc acaaccgcct cggcgcaggg atccaggcag gttacgcaca
       61 gcgcagcatg aattacagtg cgttccagtg gggaaaccaa tatgacgtaa atgcattcaa
      121 ccctaatctt ccttcgggcg aaccttccgc aggcgcagcg ttcggctatg cggatttcgg
      181 aaccggtata gtatggacct acgaaaatgc atcacgttcc gaccaggtag cgaacaacca
      241 cgagctgaaa ataaacctcg gtgccgggtt ctttcatgtg aaccagcctg cgtattcttt
      301 ttacaaaacg ccgggcgaaa aactttacat gcgcacgagc attcacggca gcgcgatcat
      361 cgggattcct tacagcagct tttcccttgt tcccggtttt atgtattcca tgcaggggac
      421 cgcgagggaa atttatgcag gcacgatgat ccgctacacc accgtgcgca cagatgtgaa
      481 atacggcaac ctgcagggga ctgcgctttc actcggcgct tacctccgca caaaagatgc
      541 ggtcgctgcg gtaatgatgc tggagtattc cagcttcgcc gcaggcttca gctatgactt
      601 caacgtttca acgctgaaag ccgcaagcag caggcagggc gggttcgaga tcgcgctgcg
      661 gtatgttttt cccgatccgt tctcgttccg ttcttccggt caatcgttct attgattgtc
      721 attttgatgt gaagaaagaa aaaacgaaac gttaacccgg cacaacatac tcctgttgtg
      781 aagtgatccg gcgttcatcc ttaactgcac gctaacaatc atgtaaaaag agcgcaggtt
      841 atttgtcatg caaaatcaat caacaacccc cgtttcatga attcattcca aaagaaatca
      901 cccgccatga tcagaagaat cacattttta agtccggcgc ttttcgccct gctgaacttt
      961 acgaacaatg cctccgcgca aatgttcaca cccgggaaca ttgtcgttga gcaggtcggc
     1021 gacggaacgt cagcgctcgc cagtggtgtt gcagcaccac tcttcctggt ggaatattca
     1081 gccaccggca cgctcaccaa ttccgttgcg cttccggtgg cgggaccgaa cgtgatcacg
     1141 accagcggta ccgcaacttc cgaaggaaat atctcgcttt ctgcaaacgg gcgctacatt
     1201 attgttcccg gttacaatgc tcttcccgga acgaccgggc ttacctccac cacttcggcc
     1261 accgttgcac gagttaccgg ccaggtggat gcatccggaa catattccat gccgtcagtc
     1321 acgaacacgt acttcagcgg caacaacatc cgcggtgcgg cgagcgacgg gaacaataat
     1381 tactgggcta ccggcgcaaa tacaggcgcg tgctatttcg gaacaggaac gccggcgatc
     1441 gtttattcaa ctaacacgaa caaccgtgcc atggccgttt acaacggcaa cctgatgttc
     1501 tctgccggcg caggaacaag aggcatttac cagttcaccg gcatgccgac gacggcaagc
     1561 gcagcttcac tcatccttcc gcttaccgga agcccttacc agttcgcgat caactccaca
     1621 tcaacgatac tttatgttgc agatgacgcc agcttcacca gcacttcttc ccaggcgggc
     1681 ggcatcgaaa aattcacctg gaacggatcg gcgtgggttt tccagtacac gctccaatgc
     1741 ggcaacgctt caactgcagg ggcacgcgga ctcgttgttg atttcagcgg aaccaatccc
     1801 gtggtctacg cttcgactgc ggaaagcagt tccaaccgca tcatttccat caccgacgcg
     1861 ggtgcatctt caggctcttc cgccacgttg ctcgccactg ccgccaccaa cacgatcttc
     1921 cgcggtcttg cattttctcc ctgctctccc ccgtcgataa caacagtgtc ttccaacagc
     1981 ccggtctgca gcggctccac actgaatctt tcttcggctg taacaggatc cgcaccgctg
     2041 acgtattcat ggaccggccc caacagcttc tccggcaaca ccgccaatcc ttctatcagc
     2101 agcgttacca ctgcagctgc cggaacgtac atgctgaacc tttcgaacgc gtgcggcagc
     2161 gcttcacaga acacgacggt cgtcgtcaac acacctcctt cgatcacaac gatcaccaac
     2221 acaagtcctg tgtgctccgg ttcggatatc aatttcaatt cagcggcaag cggaagcgca
     2281 ccgctcacat tttcatggac cggacccaac agcttttcat ccaacagcgc aacgccttcg
     2341 atcaccgggg cgacaccgct cgcgaacggg atctacacgc tcacggtgac gaacggatgt
     2401 ggcagtgata tgcagactac agtcgcattc gcttccctgc ctgttgcgtc aaccacatca
     2461 caaaccgatg tcacctgcaa cggtgccaat gacggaaccg cttccgtaac ggtttccggc
//
LOCUS       DASTSF010000509         3377 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_763047,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000509 DASTSF010000000
VERSION     DASTSF010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3377
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_763047"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            237..2054
                     /locus_tag="VFU15_14555"
     CDS             237..2054
                     /locus_tag="VFU15_14555"
                     /inference="COORDINATES: protein motif:HMM:NF016190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HEU4719061.1"
                     /translation="MKKGPGSLSGNVNARIIFFILFVLAGMALYASAGGGGGGGSDRD
                     SSGLLGTIIYYLFWLIPFPWNFVTIGAIIVIFYLARKKTKTFSGLNRIASFDSMGQQA
                     SAVSPEFLQRNPSFNEQGFLAKVSTAFSSIQVAWMNMDLSGVRRWISDGVWQRFNTQF
                     VMMKKLGQKNTMEDIRIQGMHIDRIESDGQFDIIHVGIHFTMMDNFITEKYPQLNVAG
                     RLENVEYWSFIRKSGVAEKDLYHSQNCPNCGAALPEKMGETSKCEHCGTTTTLGDYDW
                     VLSEISQAADYRNESAQLAMDGSSTAKIRQAMAGDKSFSIQSIEDKASNAYMQIMSAL
                     VTKQPERMRRFVDDKLFGQLSERIKTEPPFVFNRLFLNNVTVMNHYAAEGKDNLVVML
                     KRTAQRVSVESGQLQLIDYAPYAVNEMMILSRDAGAGDPKGSLYAHSCPSCGGPLTDT
                     LDMNCPYCGSLLNSTKTEWIVTRFDDAQGYSQLRSEGAQLATEADVEDMDALFKVRDY
                     AFNNVMVIIAADGLLHEEEIKFANQLAVKLGYDKNKIAGMIDLARNRQLVIRMPESRK
                     KCEKVYRIMEKAAAADGNVSEEERSILDDVKKHIEYMNA"
     gene            2076..2969
                     /locus_tag="VFU15_14560"
     CDS             2076..2969
                     /locus_tag="VFU15_14560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719062.1"
                     /translation="MKEFRFNYLTMSIKVTIDDFNFAAQVGPIRKYRFALSDLVNYYL
                     LVNAQYYVLMITYLNEKGKTKKLMIYSQYNEPKFKELNEELDARCGSKSLNHLDKKEA
                     LKVMKTSNPAKKGAVMGGIILFVIFSLGFYPKLLHYFDFGFDTVTVDDIASGKFPSSH
                     NVKISGHPLSQAMESTVSSDNSSSVSKTVYVPLVNESWQEGNPVKVMLEFDDISEDEY
                     NGILEQTEFVGVIRNIGWEGMKSKYVDFFKSHFSMNMPATPVLIEVTGKEHNDGWVIY
                     AWGGVLVFLGFILFIGYQKTK"
     gene            3016..>3377
                     /gene="miaE"
                     /locus_tag="VFU15_14565"
     CDS             3016..>3377
                     /gene="miaE"
                     /locus_tag="VFU15_14565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045301 -
                     tRNA-(2-methylthio-N-6-(cis-hydroxy)isopentenyl
                     adenosine)-hydroxylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA isopentenyl-2-thiomethyl-A-37 hydroxylase
                     MiaE"
                     /protein_id="HEU4719063.1"
                     /translation="MLGLKLATDPRWVNIVESNIDEILTDHAYCEQKAASNAISLIVH
                     YPEFSEMVSKLSEIAREELEHFRQVHELIVSRGGVLGKERKDDYVNELMEFTRVPNRT
                     RAEVLVDRLLFAAMIEAR"
BASE COUNT          866 a          893 c          907 g          711 t
ORIGIN      
        1 atgaaacgaa aatacgactt agaggctgtt taagaatcgt ttttggaatt tgtcaggatg
       61 catttttcgt tcagacgagg cgtattttgc aggccatagc aacgcactgc cgccaaaaac
      121 acaacgaagt atgaacggga aaggcgcata acaaaaccga aggatgattt ttaaacagcc
      181 tcttaaacca ccattcgaaa tcgtttttat tatattgcac gagtcatctg tcctccatga
      241 aaaaaggtcc tggttccctg tccggaaacg tcaacgcccg aattattttt ttcatccttt
      301 tcgtccttgc gggcatggcg ctatacgcca gcgcaggcgg cggcgggggc ggcggaagcg
      361 accgtgacag cagcgggctt ctcggcacga tcatttacta cctgttctgg ctgatccctt
      421 tcccgtggaa tttcgtgacg atcggcgcga tcatcgtcat cttctatctc gcacgaaaaa
      481 aaacgaaaac attttccggc ctcaaccgca tcgcatcgtt cgattccatg ggacagcagg
      541 cgtccgccgt ttcaccggaa ttcctgcagc ggaacccttc cttcaatgag cagggcttcc
      601 tcgccaaagt ttccactgcg ttcagctcga tacaggtggc gtggatgaac atggatcttt
      661 ccggcgtgcg gcgctggatc tccgacggcg tttggcagcg cttcaacacg cagttcgtga
      721 tgatgaaaaa gctcgggcag aaaaacacga tggaggacat ccgcatacag ggcatgcaca
      781 tcgaccgcat cgaaagcgac gggcagttcg acatcataca cgtcggcatt cattttacga
      841 tgatggataa tttcatcacc gaaaaatacc cgcagctgaa cgtggcgggc cgtctcgaga
      901 acgtggaata ctggtcgttc atccgcaaga gcggcgtggc ggaaaaagat ctttaccatt
      961 cgcagaattg ccccaactgc ggcgccgcgc ttcctgaaaa aatgggcgaa acaagcaagt
     1021 gcgaacattg cggaacgacg accacgctcg gcgattacga ctgggtgctg tcggaaattt
     1081 cgcaggcagc cgattaccgg aacgaaagcg cgcagctggc gatggacggt tcttccaccg
     1141 caaagatcag gcaggcgatg gccggcgaca aatcgttctc catccagtcg atcgaggaca
     1201 aagcgtcgaa tgcgtatatg cagatcatgt cggcgctggt gacgaagcag ccggaacgta
     1261 tgcgccgttt tgtcgacgac aaattattcg ggcagctttc cgaacgcata aagaccgaac
     1321 cgcccttcgt gttcaaccgc cttttcctga acaacgtgac ggtgatgaat cattacgccg
     1381 ccgaaggaaa agacaatctt gtcgtgatgc tcaagcgcac cgcgcagcgc gtttctgtcg
     1441 aatccggcca gctgcagctg atcgattatg caccctacgc ggtgaacgag atgatgatcc
     1501 tttcacgcga tgcaggcgcc ggcgatccga aaggctcgct ctatgcgcac agctgtcctt
     1561 cctgcggcgg accgctcacg gatacgctcg acatgaactg tccgtattgc ggatcgctgc
     1621 tgaacagcac gaaaacagaa tggatcgtca cacgcttcga tgatgcgcag ggttattcgc
     1681 agctgagaag cgaaggcgcg caactggcga cggaagcgga tgtagaggac atggacgcgc
     1741 tgttcaaggt gcgcgattat gcgttcaaca acgtgatggt gatcatcgcc gccgacggcc
     1801 tgttgcacga agaagagatc aaattcgcga accagctggc cgtaaagctc ggttacgaca
     1861 aaaacaagat cgccggcatg atcgaccttg ccaggaaccg ccagctggtg atccgcatgc
     1921 cggagtcgcg gaaaaaatgc gagaaggttt accgcatcat ggaaaaagcc gctgccgccg
     1981 acgggaatgt cagcgaagag gagcgttcca ttctcgacga cgtgaaaaaa cacatcgaat
     2041 acatgaacgc ctgaccccta aaaaattctg ctcccatgaa agaattccgc ttcaattacc
     2101 tgaccatgtc gatcaaggtg acgatcgacg atttcaactt cgccgcccag gtggggccga
     2161 tacggaaata ccggttcgcg ctttccgacc tcgtcaatta ttatctcctg gtgaatgcgc
     2221 agtactatgt gctcatgatc acgtacctca acgaaaaagg caagaccaaa aagctcatga
     2281 tctacagcca gtacaatgag ccgaagttca aagagctgaa cgaggagctg gatgcgcggt
     2341 gcggttccaa aagtctgaac cacctcgaca aaaaagaagc gctcaaggtg atgaaaacat
     2401 cgaaccctgc gaaaaaaggc gcagtcatgg gcggcattat cctgtttgtc attttctcgc
     2461 tcggttttta cccgaagctc ctgcactatt tcgatttcgg tttcgacacc gttaccgtgg
     2521 acgatatcgc cagcgggaaa ttcccttcct cgcacaacgt gaagatcagc ggccacccgc
     2581 tcagccaggc catggaatcc acggtcagca gcgacaattc ctcttccgtc agcaaaacag
     2641 tttatgttcc gctggtgaat gaaagctggc aggaaggcaa cccggtgaag gtgatgctgg
     2701 agtttgatga tatttcagaa gacgaataca acggcatact ggaacagacg gagttcgtgg
     2761 gcgtcatccg caacatcggg tgggaaggaa tgaaaagcaa gtacgtggat tttttcaaat
     2821 cgcatttcag catgaacatg cccgcaacgc ccgtgctgat cgaagtgacg ggcaaagagc
     2881 ataatgacgg ctgggtgatc tacgcctggg gcggggtact cgttttcctg ggcttcatcc
     2941 tgttcatcgg gtaccagaaa acgaaatgat tctgctttcg ctgcagaact ttttttatga
     3001 aaaaaaccct tcgccatgct cggactgaag cttgccactg acccacgctg ggtgaacatc
     3061 gtcgaaagca atatcgatga gatcctcacg gatcacgcgt actgcgaaca gaaagcggcg
     3121 tcgaacgcca tttcgctcat cgtgcattat cccgaatttt cagaaatggt ttcgaagctt
     3181 tcggagatcg cgcgcgaaga gctggaacat ttccgccagg tacatgagct catcgtcagc
     3241 cgcggcggcg tgctcggcaa ggagcggaag gacgattacg tgaacgaact gatggaattc
     3301 acccgcgtgc ccaaccgcac gcgcgcggaa gtgctggtcg accggctgct gtttgccgcg
     3361 atgatcgagg cgcggag
//
LOCUS       DASTSF010000510         2813 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_819149,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000510 DASTSF010000000
VERSION     DASTSF010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2813
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_819149"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(150..464)
                     /locus_tag="VFU15_14570"
     CDS             complement(150..464)
                     /locus_tag="VFU15_14570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008507550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEU4719064.1"
                     /translation="MKEITVQELKRMKDSGEKFQLIDVREPHEAEIASIGGELIPMGE
                     ILSQAERLAKDIPVVVHCRSGARSASVIQALEKQGFSNLLNLKGGILAWAKEIDTSLS
                     TY"
     gene            complement(480..1259)
                     /locus_tag="VFU15_14575"
     CDS             complement(480..1259)
                     /locus_tag="VFU15_14575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019595966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphosulfolactate synthase"
                     /protein_id="HEU4719065.1"
                     /translation="MNVNLPHVPDRPVKPRKSGLTMVMDKGLGLKQAELLMETNTDFV
                     DLVKLGFGTSTVTRNTTEKIKLYKEAGMKVYLGGTLFEAFYIRGMVDDYIRFAEKLKL
                     DHAEVSDGSVVMKHSEKCELIAKLSKHFTVLSEVGSKEEGIIIHPNKWTSMMLKEIEA
                     GSWKVIAEARESGNVGIYHRNGSAHTMLVNKIITKVKPENIIWEAPQKSQQVWFIKHF
                     GPNVNLGNISFEEVIPLETLRMGLRGDTFFTFLPDKMKKTN"
     gene            complement(1314..2477)
                     /locus_tag="VFU15_14580"
     CDS             complement(1314..2477)
                     /locus_tag="VFU15_14580"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU4719066.1"
                     /translation="MGENRVALDILNEAERMEPFYGDIHMTRGSIYGQMGLHEKAITS
                     YTLAGQYDIPEDEVKYWCAYELGNMGRHEEALPLLKKILLKNPDHEAAMSELAFCYEA
                     FADIDDSIRFFTKMTDLRPYNSLAWFNLGVAYSRAADYEKAIGAYDYALLIREDFASA
                     IFNKAHALATLGRHDEAIQLFLETFRYEEQRGDTHYYIGESYEKKEEWDLAMIHYNKA
                     IKLSPDLADGWLGMGLCLEMLGRMQEAIHYVKKAIEIAPDNSEYWYTFGDIQVKLGFQ
                     EEAEAAYRKVLEIEPENPDIWLDYSGLQFEMENEDIALETLAEGIKFHPSNAELNYRM
                     SAYLLSLGKKQDALSYLHNALQLDFEKHNTLFEFLPQLKENTGLAEIIESYRK"
BASE COUNT          638 a          708 c          711 g          756 t
ORIGIN      
        1 tttccacgaa aatgatcagg aaaagaatgg cgtagatcgc agtgccgtat tcggccacca
       61 tgaaatcaag gcgatcgttg atgtgcagga caaaatcgat caggctgtgc aggaatccca
      121 ttgaaggacg ctggaaagtg aaatgaggat cagtaagtgg aaagagaagt gtcgatctct
      181 tttgcccagg ccaggatccc gcctttgagg ttgagcaggt ttgagaagcc ctgcttttcc
      241 agcgcctgta tcacgctggc ggaacgggcg ccgctgcggc aatgaacaac aacaggaatg
      301 tctttcgcaa ggcgctctgc ctgggaaagt atttctccca tcggtatcag ctctccgccg
      361 atggaagcaa tctcggcttc atgcggttca cgaacgtcga taagctggaa tttttcaccg
      421 gaatctttca tccgcttcag ttcctgcact gtgatctctt tcataaaaat ggattggtct
      481 taattcgttt ttttcatttt gtcgggcagg aacgtgaaga atgtatcgcc gcggagaccc
      541 atgcgaagcg tttccagcgg gatcacctct tcgaatgaaa tgttcccgag gttgacgttg
      601 ggtccgaaat gcttgatgaa ccacacctgc tgtgatttct gcggtgcttc ccagatgatg
      661 ttctccggct tcactttcgt gatgatcttg ttcacgagca tggtgtgcgc cgatccgttg
      721 cggtggtata tgcccacgtt gccgctttca cgcgcttccg cgatcacctt ccacgagccg
      781 gcttcgatct ctttcagcat catcgatgtc cacttgttcg ggtgaatgat gatgccttct
      841 tctttcgatc ccacttccga aaggacggtg aaatgcttcg acagcttcgc gatgagctcg
      901 catttttcgc tgtgcttcat caccacggaa ccatcggaaa cttctgcatg atcgagcttc
      961 agtttttccg cgaaacggat gtaatcatcc accatgccgc ggatataaaa ggcctcgaaa
     1021 agggtgccgc cgagatacac tttcatgccg gcttccttgt acagctttat tttttccgtg
     1081 gtgttccggg tgacggtgga tgttccgaaa ccaagcttca ccaggtcgac gaaatcggtg
     1141 ttcgtttcca tcagcaattc ggcctgcttg agaccgaggc ctttgtccat gaccatggtg
     1201 agccccgatt tgcgcggttt gaccggtctg tcgggtacgt gagggagatt gacgttcatg
     1261 ggctgattat ttcccgcaaa actatgcaaa aagaaaacgt tacggccgga atcttacttg
     1321 cggtaggatt cgatgatctc ggcaagaccg gtattttctt tcaattgcgg aagaaattcg
     1381 aataaagtgt tgtgcttctc gaaatcgagc tgaagcgcgt tatgaagata agaaagggca
     1441 tcctgcttct tgccgagact gagcaggtaa gccgacatgc ggtaattcag ctcggcattg
     1501 gacggatgga acttgatgcc ttcggccagc gtttcgaggg caatgtcctc gttctccatt
     1561 tcaaactgca gtccggaata atccagccag atgtccgggt tctccggttc gatctccagc
     1621 actttgcggt acgccgcttc cgcttcttcc tggaaaccga gcttcacctg tatatcgccg
     1681 aacgtgtacc agtattcgct attgtcgggt gcgatctcga tggctttttt cacgtagtga
     1741 atagcttcct gcatgcgtcc cagcatttcc aggcaaagac ccatgcccag ccagccgtcg
     1801 gccaggtcgg gagaaagctt gatcgctttg ttgtaatgga tcatggcgag gtcccattcc
     1861 tctttttttt cgtacgactc gccgatgtaa taatgtgtgt cgccccgctg ctcctcgtaa
     1921 cggaaggttt cgaggaaaag ctgtatcgct tcatcgtgcc tgccgagcgt agccagcgcg
     1981 tgcgctttat tgaaaatggc cgatgcaaaa tcctcgcgga tcagcagcgc ataatcataa
     2041 gcgccgatcg ctttttcata atcggccgcc ctgctgtatg ccacgccgag attgaaccag
     2101 gcgagggaat tatacggacg caggtccgtc atctttgtaa aaaagcggat gctgtcgtcg
     2161 atatcggcaa aagcttcgta gcagaacgcg agctcgctca tcgcagcttc atgatccgga
     2221 tttttaagaa ggatcttttt gagcagggga agcgcctctt cgtgccggcc catgttgccc
     2281 agctcgtaag cgcaccagta tttcacctcg tcttcaggaa tatcatactg gcccgcaagc
     2341 gtatacgacg tgatggcctt ttcatgcagg cccatttgtc cgtaaatgct tccgcgcgtc
     2401 atgtgaatgt caccgtaaaa cggttccatc ctttccgctt cgttaagtat atcgagcgct
     2461 accctgtttt cgcccatgtg cacatgaacc tgtgccttga ggacaagaaa aaggggagaa
     2521 aagggatgtt gctgacgtgc gaggtcaagc aactcgagac atctgtttaa ttgcttgtta
     2581 gccagatagt aatcggcgat ctcttcaagt tcttcttctt caaaaaatga aaagctttga
     2641 agccgtttca tttcttcgta ccgctcaatc aacttctcgt ttggagtttt ctcacgatcc
     2701 atagaacggg tattgatttt acttctaaac aaagatacgt tatacgcccc ggaaagttgg
     2761 gataagcatc ccaaaagttg gtaaagtttt caccaaaagc cggaaatcct gtg
//
LOCUS       DASTSF010000511         7383 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_46994,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000511 DASTSF010000000
VERSION     DASTSF010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 7383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7383
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46994"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..762)
                     /locus_tag="VFU15_14585"
     CDS             complement(<1..762)
                     /locus_tag="VFU15_14585"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU4719067.1"
                     /translation="MSEVSGKAIDFKLLKRILGYTKPYRGLFSAAITLTITLSFLSIV
                     RPMLVRYTMNSYVHLPGSASDEDFLKTKLILFCSLMFGLLVLESAMQFVNSYSTSLLG
                     QRIVKDLRVQLNKKVLHFRSGYFDQTPIGMLVTRVISDIEAINDVFSQGFIVIAGDLL
                     TIIVYLVAMLIVDWRVTLVVLSTVPVMFFSTWLFKNAVKASFTDVRNQVSRLNTFVQE
                     HITGMKIVQVFNREKVEMDRFMDINKKHRDANIKSV"
     gene            complement(755..1552)
                     /gene="truA"
                     /locus_tag="VFU15_14590"
     CDS             complement(755..1552)
                     /gene="truA"
                     /locus_tag="VFU15_14590"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013633515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEU4719068.1"
                     /translation="MSRFHRYFIRLSYNGTGFCGWQYQPGMATIQETVEEKLGIMLRE
                     KIDVVGCGRTDSGVHAKKYFAHFDSIAEGLDKDEKVIFRLNKMLPAGIAIQKIHAMAD
                     NAHARFAAVARSYEYHISRVKDPFGPDLSWELHEQLDVNAMNECARIIPAYNDFAAFS
                     KTGSDNKTTLCKVTVSYWEEKGDKLIYHITANRFLRNMVRAIVGTLVEAGKGRISPGD
                     VRRIIESGKRSEAGQSVPAEGLYLSDVRYPAAFQLEEEESVIQAAHE"
     gene            1950..2939
                     /locus_tag="VFU15_14595"
     CDS             1950..2939
                     /locus_tag="VFU15_14595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEU4719069.1"
                     /translation="MINDIQAVNERIRQESAFVDKINAELDTAIVGQKTMIERLLIAL
                     LSNGHILLEGVPGLAKTLAVKSLAATIHADFSRVQFTPDLLPADLVGTMIYNQKQEKF
                     TVRRGPIFANFVLADEINRAPAKVQSALLEAMQERQVTIGDETFPLPEPFLVLATQNP
                     IEQEGTYPLPEAQIDRFMLKVVIRYPTKDEEQRIIRQQLAASPRKPQAILHPEDILRA
                     RKVVQDVYLDEKIEKYIVDLVFATRFPNDYRLQKLQPMIAFGGSPRASISLAQAAKAY
                     AFLQHRGFVIPDDIRAVAYDVLRHRIGLTYEAEAENVTTEMIINEVLATVHVP"
     gene            2965..3849
                     /locus_tag="VFU15_14600"
     CDS             2965..3849
                     /locus_tag="VFU15_14600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010527407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEU4719070.1"
                     /translation="MSTTAEILKKVRKIELKTRGLTSQVFSGEYHSAFRGRGMTFSEV
                     REYQPGDEVRTIDWNVTARLNHPYVKVFQEERELTVMLLVDVSRSGFFGTHAQLKQEL
                     ITELAAVLSFSAIQNNDKAGVIFFSDKVEKFLPPKKGKPHILRIIRESIEYKPSGNGT
                     DIAAALEYCTNAIKNRCIVFILSDFMSGGFEEAMRIANRRHDVVALHVYDRRETELPD
                     IGLVQLVDNETGDLKWVDTSDPGVRTKYARAARTHRLYLDDTFRKSGIDAAHIATGDH
                     YIKTLSALFRKREKRVSK"
     gene            3852..4823
                     /locus_tag="VFU15_14605"
     CDS             3852..4823
                     /locus_tag="VFU15_14605"
                     /inference="COORDINATES: protein motif:HMM:NF024973.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HEU4719071.1"
                     /translation="MLRLLVFFTCIFSCAGLHAQRVEAEAVLDRTNFRIGEQVNLKLA
                     VHYMEGSQKSVVTWPEVSDTLVKGVEIVKDDSIHTKLVNRASVLYEQAKTLVITAFDS
                     GNYTIPALRFIVDRDTVMTDPVAFYVATVPVDTTKPIRDIKDIYSDIPDAPPVNEGKS
                     SSPWIWIVSGLLSLLLIAGVIYFATRKKTAPVPLQPARHLFPHEKVLEMLAELGRRKP
                     WLHGELKPYHVALTEIMRGWLVERFGIHAREMTTAEIIRVLSAMRNDPGNIMKLERVL
                     RTADLVKFAKEIPADEENESSLSLAMSFVQATAAHNEFSNPVSRGVQ"
     gene            4820..5833
                     /locus_tag="VFU15_14610"
     CDS             4820..5833
                     /locus_tag="VFU15_14610"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEU4719072.1"
                     /translation="MSHWWYSIAFADKWVLWFLTGLPVAGFFAWYAGRRGKPALALSS
                     MRYLKNIPVPAKVKWKPVMYFFRLAAIALLIMACARPQSVSPWRNDHTKGIDIMLAID
                     VSPSMQANDFYPTRMEAAKAEAEKFVDDRPNDRIGVVIFSGETFTLCPLTADHDALKT
                     MIENINPGSLDMGTAIGMGLAKSVERLKSSDAKSKVVVLLTDGENNAGVISPVDAARL
                     AKTFHVKVYTIGMGAMGGKVLMPTAQNPDGSYVQQYTNVDIDEKTLMDIASTTGAKYF
                     RASDTKKLDEIYSEINTLEKSDVDKKERQDKKEEFLPLLFGAVFFLLVEVVFRYTIFN
                     SVT"
     gene            5830..6852
                     /locus_tag="VFU15_14615"
     CDS             5830..6852
                     /locus_tag="VFU15_14615"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEU4719073.1"
                     /translation="MTLLSPMLLFFLALAVPVLLLWYHSVRLRQERISRFAESAFTGK
                     LLSGYSRGLGLWRFWMVFAAMILMLFSLAAPMIIGGRERVKKTGIDVVIALDISNSMR
                     ANDIKPSRLESVKLALSDMIAKMGDDRLGLVIFAGQAYTSVPLTQDHSAAQMVVQSLD
                     PDMISLQGTAIGNAIEQSLLPFRDEDTRRGKAIILISDGENHEDDPIAATKKAVEKNV
                     IVNTIGIGSDAGAKIPLYTPSGDFIENKKDGNGQEIVTKLDEKTLRDIAKEGHGIYVR
                     ATQSDIGIDKVYDSLRGLNKSTEDAWRYTDLSPLYRWFLLGAIVILLAEMFIPEGKRK
                     EILKRS"
     gene            6932..>7383
                     /locus_tag="VFU15_14620"
     CDS             6932..>7383
                     /locus_tag="VFU15_14620"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU4719074.1"
                     /translation="MKRMLTVFTLLFFFSVANAQQPAQKDAWKNTRDGNQSFKAGNFV
                     QAEKHYREAVHSDTNKVVANYNLGNSLYRQRKYDDASRAYADGLNTKNADSLAKGYHN
                     LGNALLQEKKYQESINAYKQSLKLNPKDENTRYNLAYAESKLKQNQPPP"
BASE COUNT         1793 a         1945 c         2027 g         1618 t
ORIGIN      
        1 cacggatttt atattggcgt cacggtgctt cttgttgatg tccatgaacc ggtccatctc
       61 cactttttcg cggttgaaca cctgcacgat cttcatgccc gtaatgtgct cctgcacgaa
      121 cgtgttgagc cgcgacacct ggttgcgtac gtcggtgaaa gaagctttca cggcgttctt
      181 gaaaagccat gtggagaaaa acatcacggg caccgtcgaa agcaccacca gcgtcacgcg
      241 ccagtcgaca atgagcatgg ccacgaggta aacgatgatc gtgagcaggt cgccggcaat
      301 gacgatgaat ccctgcgaga agacgtcgtt gatcgcttcg atatcggaaa tgacacgcgt
      361 aacgagcatg ccgatcggcg tctggtcgaa atagccggaa cggaaatgca gcactttttt
      421 gttgagctga acgcgcaggt ctttcacgat gcgctgcccg agcaggctgg tgctgtagct
      481 gttcacgaac tgcatggcgg attccagcac gagcaggccg aacatcagcg agcagaagag
      541 gatgagtttt gttttcagga aatcctcgtc ggaagcgctt ccggggagat gaacgtagga
      601 attcatcgtg tagcgcacga gcatgggacg aacgatgctg aggaaggaaa gcgtgatcgt
      661 cagcgtgatc gcagcggaaa acaatccgcg gtacggtttc gtatagccga gaatacgttt
      721 cagcagcttg aaatcgatcg cttttccgga aacttcactc atgtgcagct tgtattacgg
      781 attcttcttc ttccagctga aaagcggccg ggtaacggac atcggaaagg taaagtcctt
      841 ccgccggaac cgactggccc gcttccgaac gtttaccgct ttcgatgatg cggcgcacat
      901 ctccgggtga aatccggcct ttgccggctt ccacgagcgt gcccacgatg gcgcgcacca
      961 tgttgcggag gaaacggttg gcggtaatat ggtagatgag cttgtctcct ttttcttccc
     1021 agtacgaaac agtcactttg cagagcgtcg ttttgttgtc gcttcctgtt ttgctgaacg
     1081 ctgcaaagtc gttgtacgcg ggaataattc gtgcgcattc gttcatcgcg ttcacgtcga
     1141 gctgttcgtg cagttcccag gaaaggtcgg gaccgaacgg atctttcacg cggctgatgt
     1201 ggtattcgta gctgcgggcg accgccgcga aacgtgcgtg cgcattatcc gccattgcgt
     1261 ggattttctg tatggcgatt cctgcaggca gcattttatt cagccggaag atcacttttt
     1321 cgtccttgtc cagtccttcg gctatgctgt cgaaatgcgc gaaatatttt tttgcatgca
     1381 cacccgaatc ggttcttccg caacctacca catcgatctt ttccctgagc atgatgccga
     1441 gcttttcctc cactgtttcc tgtatcgtcg ccatgcccgg ctggtactgc cagccgcaga
     1501 agccggttcc gttgtacgaa agccggataa aatagcggtg aaaacgtgac atgagcggtt
     1561 gcggaaaatt cctgtgcaaa ggtagtcaaa aggaaactga aagatgttgg tacagcgatc
     1621 aaacattacc cgcattcccg gtattcattt gccttgcaga gagttgtttt tgcagcacgc
     1681 cgggtttcaa cctgcgctca gtctcgcagt gcgcagcctc gtaccacttc atccgggtta
     1741 ttcacccggc gcttcatctc tgaactgttc tttctttcac gtgtcaccaa accggaaacg
     1801 tgaaaccagg accccggaat tttccggctt taaaactttg aactttccaa tggtgcggat
     1861 cgttaacata attttacacc ccctcactct acttaggaga ccttccggtt aactttgtcc
     1921 gtagcacacc aaagcgaaaa tttcttctta tgataaatga tatccaggct gtcaacgaac
     1981 ggatacggca ggaaagcgcc ttcgtggaca agatcaatgc ggaactcgat acggccatag
     2041 tcggtcagaa aacaatgatc gagcgcctgc tcatcgcgct gctctccaac ggccacatcc
     2101 tgctcgaagg tgttccgggt cttgcgaaaa cgctcgccgt gaaatcgctg gcggccacta
     2161 ttcatgccga cttcagccgt gtgcagttta cgcccgatct tcttcccgcc gatctcgtcg
     2221 gtacgatgat ctacaaccag aagcaggaaa aattcaccgt gcgccgcggt cccatctttg
     2281 cgaacttcgt tcttgccgat gagatcaacc gtgcgccggc aaaagtgcag agcgcgctgc
     2341 tcgaagcgat gcaggagcgc caggtcacca tcggcgacga aacgttcccg ctgcccgaac
     2401 cttttctcgt gctcgccacg cagaatccca tcgagcagga aggaacgtac ccgctgcccg
     2461 aagcgcagat cgaccgcttc atgctgaagg tggtgatcag gtacccgacg aaggacgagg
     2521 agcagcggat catccggcag caactcgccg catcgccgcg caaaccgcag gcgatacttc
     2581 atcccgaaga tattctccgt gcgagaaaag tcgtgcagga tgtttacctc gatgagaaaa
     2641 ttgaaaaata catcgtggat cttgtttttg ccacgcgctt cccgaacgat taccgcctgc
     2701 agaaactgca gcccatgatc gcgttcggcg gatctcctcg cgcttccatc agtcttgcgc
     2761 aggcggcaaa agcgtacgcg tttctccagc accgcggttt cgtcatcccc gacgatatcc
     2821 gtgctgtcgc gtacgatgtg ctccgccacc gcatcgggct gacctacgaa gcggaagccg
     2881 agaacgtgac gacagaaatg atcatcaatg aagtgcttgc gacggttcac gttccgtgag
     2941 cacgcaggat aaaagcggaa gacgatgagc accacggcgg aaattctgaa gaaagtcagg
     3001 aagatcgagc tgaaaacgcg cggcctcacc agccaggttt tttcaggcga atatcattct
     3061 gctttccgcg gaaggggaat gacattttcc gaagtgcgcg aataccagcc cggcgatgaa
     3121 gtgcgtacga tcgactggaa cgtgaccgcc cggctcaacc atccgtacgt aaaggttttc
     3181 caggaagagc gcgagctcac cgtgatgctg ctcgtcgatg tcagccgctc cggttttttc
     3241 ggaacgcatg cgcagctcaa gcaggaactt atcaccgagc tggccgccgt gctttctttc
     3301 tccgccatcc agaacaacga caaggccggc gtcatctttt tcagcgacaa agtggaaaaa
     3361 ttcctgccgc cgaaaaaagg gaaaccgcat atcctccgca tcatccgcga atcgatagag
     3421 tacaaacctt ccggcaacgg aaccgacatt gccgctgcgc tcgaatactg caccaacgcg
     3481 atcaagaacc gctgcatcgt tttcattctt tcggatttca tgagcggcgg tttcgaagaa
     3541 gcgatgcgca ttgccaaccg gcgccacgat gtggtcgcat tgcacgtgta cgaccggcgc
     3601 gaaacggagc tacccgacat cggccttgtg cagctcgtcg acaacgaaac cggggacttg
     3661 aaatgggtcg acacgtcgga tcccggtgtg cggacgaaat acgcacgtgc agcacgcaca
     3721 caccgtcttt acctcgacga tacgttccgt aaatcaggta tagacgccgc acacatcgct
     3781 accggcgatc attatatcaa aacattgtcc gcgctcttcc ggaaaagaga gaaaagagtt
     3841 tcgaaatgag aatgcttcgc ctcctggttt tcttcacctg cattttttcc tgcgcaggtc
     3901 tgcacgcgca acgtgtggag gcggaagctg tgctcgaccg gacgaatttc aggataggcg
     3961 agcaggtaaa cctgaagctg gcggtccatt acatggaagg ttcgcagaaa tcagttgtga
     4021 cgtggcccga agtttccgat acgctggtga aaggtgtgga gatcgtaaaa gacgatagca
     4081 ttcacacgaa gctcgtcaac cgcgcttcgg tcctgtacga gcaggcgaaa acgcttgtca
     4141 tcaccgcatt cgattcgggg aattatacca tcccggcgct gcgtttcatt gtcgaccgcg
     4201 atacagtgat gacggatcct gtagcgtttt atgttgccac tgttcccgtc gacacaacaa
     4261 aaccgatccg tgacatcaag gatatttaca gcgacattcc ggacgctccg ccggtgaacg
     4321 aaggaaaaag cagcagtccg tggatatgga tcgtttccgg gcttctttcc ctgttgctca
     4381 tcgcgggtgt tatttatttc gctacgcgga aaaaaacggc accggttccg ctgcagcctg
     4441 cgcgtcatct ttttccgcat gagaaagtgc tggaaatgct ggccgaactc gggcggagaa
     4501 aaccctggct gcacggggag ctgaagccgt atcatgttgc gcttacggag atcatgcggg
     4561 gctggctggt ggaacgtttc ggcattcacg cgcgggaaat gacgacggca gagatcatcc
     4621 gcgtgctcag tgcgatgagg aacgacccgg gaaacatcat gaagctggaa cgtgttctcc
     4681 gcacggccga cctggtgaaa ttcgcgaaag agatcccggc ggatgaagag aacgaaagca
     4741 gcctcagcct cgcgatgagt ttcgtgcagg ccactgcggc gcacaatgaa ttttctaacc
     4801 cggtaagcag gggtgtacaa tgagtcactg gtggtatagt atagcgtttg ccgataagtg
     4861 ggtgctctgg ttcctcaccg gactcccggt tgcaggtttt ttcgcctggt acgcggggcg
     4921 gcggggaaaa cctgcgctgg cgctttcgtc catgcgttac ctgaagaata ttcccgttcc
     4981 tgccaaagtg aaatggaagc cggtgatgta tttttttcgt ctcgctgcga tcgcgctgct
     5041 catcatggct tgcgcgcggc cgcagtcggt gagcccgtgg agaaacgatc atacgaaggg
     5101 catcgacatc atgctggcga tcgatgtttc accgagcatg caggcgaatg atttttaccc
     5161 gacgcgcatg gaagcggcga aggcggaagc ggaaaaattt gtcgacgacc gtcccaacga
     5221 ccgcatcggt gtcgtcattt tcagcgggga aacattcacg ctttgcccgc ttacggcaga
     5281 ccatgatgcg ctgaagacga tgatcgaaaa catcaacccg ggatcgctcg acatgggaac
     5341 cgcgatcggc atgggactgg cgaaatcggt tgagcgcctg aagagcagcg atgcgaaaag
     5401 caaagtggtg gtgctgctga cggacggtga aaataacgca ggtgtaattt ctcctgtgga
     5461 tgcggcgcgg ctggccaaaa catttcatgt gaaagtatat acgatcggga tgggagcgat
     5521 gggcggaaaa gtgcttatgc ccacggcaca gaatcccgac ggaagttatg tgcagcaata
     5581 caccaatgtc gacatcgacg agaaaacgct catggatatt gcatcaacga caggcgcaaa
     5641 atatttccgc gcttcggaca caaaaaagct ggatgagatc tacagcgaga taaatacgct
     5701 cgaaaaatcc gacgtcgata aaaaggaaag gcaggataaa aaagaagaat ttcttccgct
     5761 gcttttcggc gccgtgttct tcctgctcgt cgaagttgtt ttcaggtata ccatattcaa
     5821 cagcgtgaca tgacactatt atcacccatg ctgctttttt ttcttgcgct cgccgtgccg
     5881 gtgctgttgc tgtggtatca ttcggtcagg ttgcgccagg aaaggatcag ccgtttcgcg
     5941 gaatcggcgt tcacgggaaa gctgctgagc ggttacagcc gcgggctcgg gctctggcgt
     6001 ttctggatgg tctttgctgc gatgatcctc atgctgtttt cgctcgcggc gccgatgatc
     6061 atcggggggc gtgagagagt aaaaaagacc ggcatcgacg tcgtcatcgc gctggatatt
     6121 tccaacagca tgcgcgcgaa cgacattaaa ccgagccgtc ttgaaagcgt caagctcgcg
     6181 ttgtcggaca tgatcgcgaa gatgggcgat gatcgtctcg gcctcgtgat cttcgccggg
     6241 caggcgtaca ccagtgttcc gctaacgcag gatcattccg ctgcgcagat ggtggtgcag
     6301 tcgctggatc cggatatgat ctcgctgcag ggaaccgcga tcgggaatgc gatcgagcag
     6361 tcgcttcttc cgttcaggga cgaagacacg cggagaggaa aagcgatcat cctgatttcc
     6421 gatggagaaa accacgagga tgatccgatc gccgccacca aaaaagcggt ggagaagaac
     6481 gtgatcgtga atacgatcgg catcggctct gatgcagggg cgaagatccc gttgtatacg
     6541 ccgtccggcg attttattga aaacaaaaaa gacgggaacg ggcaggagat cgtgacgaag
     6601 ctcgatgaaa aaacgttgcg cgatattgcg aaagaaggac atggcattta cgtccgcgcc
     6661 acgcaatcgg atatcggcat cgataaagta tatgacagtc tccgcgggct gaacaaatcg
     6721 acggaggacg catggcggta tacggatctt tctcccttgt accggtggtt tctcctcggt
     6781 gcgatcgtta tccttcttgc ggaaatgttc attccggaag ggaaaagaaa ggaaattcta
     6841 aaaagaagct gaaatggaaa aaggaaactt gttcccccga atattttttc aggtgcagct
     6901 gtctgccccc gaaaaaaaca acgcaccggt catgaagcga atgctgaccg tctttacgct
     6961 cctgttcttt ttcagcgttg caaatgcaca acagcctgcg cagaaagatg cgtggaaaaa
     7021 tacgagggac ggaaaccaga gcttcaaagc cgggaatttt gtgcaggccg aaaaacatta
     7081 ccgggaagcg gtgcacagcg atacgaacaa ggtggtggcc aattacaacc tcgggaattc
     7141 cctctaccgc cagaggaaat acgatgacgc cagccgcgcc tatgctgacg gactgaacac
     7201 gaaaaatgct gattcgctcg caaaagggta tcacaatctc ggcaacgcgc tcctgcagga
     7261 gaaaaaatac caggaaagca tcaatgccta caagcaatcg ctgaagctga atcccaaaga
     7321 cgagaatacg cgttacaatc tcgcttacgc cgaatcgaaa ctgaagcaga accagccacc
     7381 gcc
//
LOCUS       DASTSF010000512         7060 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_986755,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000512 DASTSF010000000
VERSION     DASTSF010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 7060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7060
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_986755"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..743
                     /locus_tag="VFU15_14625"
     CDS             <1..743
                     /locus_tag="VFU15_14625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719075.1"
                     /translation="SVWLVTKEEKRGTEEGTWRGAVDERTGTLWLGRNEHLEKNPPPA
                     GEYWKNVPVTRALLRQVFSDLSFSQAWLLAGYGSVLARKKLSWNAGEFAKWEQERKET
                     LAELAADLDPETAIHAFAEMMKDPGDVKYDAADFAELFGQVARRDEDKMLSVLERTLQ
                     EPAGMDRDEIALALGEAGLEEWLKLADETAAEDPPENYEDDMDVPLICADCFAGKPAC
                     FDAVGKKIPPGNRRLERYHALLRKLYAA"
     gene            810..2027
                     /locus_tag="VFU15_14630"
     CDS             810..2027
                     /locus_tag="VFU15_14630"
                     /inference="COORDINATES: protein motif:HMM:NF026220.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HEU4719076.1"
                     /translation="MKKIYTIFSVCIALSASAQTITWNQQTSASTNDLYGVSFTDANH
                     GWTAGLGGVIQNTTDGGNNWALQNSGITTNLRSIFFIDNNNGWACGDGGVILHTSNAG
                     TSWAAQTSTTTNNLRSIQFTDANNGWCAGIAGTILHTGNGGTTWTAQTSTSTQGLMRI
                     HFASTTTGWVAGSSAPSTSGFALSSTNGGGTWSSQTISASYNFFGLDFASATTGWICG
                     TNGSVYKTTNGGTSWSQQTTGMSHTMRGVSFPTLNDGWMVGDSGYIYITSNGGTSWTQ
                     QQTYMISFMRDVHFVSVNAGWAVGDHGTILRIVDPSGISSKDQFADFIRLFPDPSNGK
                     FEMSVTVKSATTLSWSVMDMSGRTIMQVRTDISAGTTSVPVELPEGTDKGMYILTGST
                     AGGEQFAEKFSVQ"
     gene            2224..4119
                     /locus_tag="VFU15_14635"
     CDS             2224..4119
                     /locus_tag="VFU15_14635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008242966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEU4719077.1"
                     /translation="MSDAIKHECGIALIRLLKPLQYYIDKYGSPMYGVNKLYLLMEKQ
                     HNRGQDGAGVATIKFDVPPGTRYISRYRSTSPTAIKDIFGKISDKFSDGYKKNAEKMK
                     DATWVKRHVAFSGELLLGHLRYGTYGGNSIENCHPFLRQNNWITRNLVVAGNFNLTNV
                     DELFHHLVELGQHPKEKADTVTVMEKIGHFLDREVERLFQHYKVLGHSNQEITDLIAR
                     DLDIQKILKDAGKRWDGGYAMAGLLGHGDAFVLRDPNGIRPAFWWANDEVVVVASERP
                     QIMTAFNAQLEEIKEITPGSALIIRRNGHYEEKGVRTPREKKSCSFERIYFSRGNDKD
                     IYEERKNLGRQLTPTVLKSVAFDLKNTVFSFIPNTAEVAFYGLVEGLHNYLNTIKRHK
                     ILTMKNVADPELSEILNMQPRVEKIALKDAKLRTFITSDSQRDEMVSHVYDTTYGVVN
                     PGDNLVMLDDSIVRGTTLRQSIVKILDRLAPKKIVIVSSAPQIRYPDCYGIDMARMGD
                     FVAFRAAVELLKDTYQENILEEVYEKAKAQEDLPANEVKNVVKEIYKPFTADEISQKI
                     SELLTPPEVSANVEVIYQSIEGLHTACPGHTGDWYFTGDYPTPGGNKVVNRAFIYFME
                     GKNVRAY"
     gene            4306..4581
                     /locus_tag="VFU15_14640"
     CDS             4306..4581
                     /locus_tag="VFU15_14640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719078.1"
                     /translation="MSRIRFRPERPWIFRRPAFIVFGIVFFLLLILAGLASNEMYNKY
                     IDRKWPPDWSLILKPLWQAPIIALVFYACAVAIWFILWVRSQGGRLL"
     gene            4688..>7060
                     /locus_tag="VFU15_14645"
     CDS             4688..>7060
                     /locus_tag="VFU15_14645"
                     /inference="COORDINATES: protein motif:HMM:NF024220.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine-rich protein"
                     /protein_id="HEU4719079.1"
                     /translation="MKNKLLTAMAVGLSVYSLAQTTTTFNYTGAVQTFTVPSCVTSIT
                     IEAYGAQGSNATTGGTGGLGGYAVGTMNVLPGDVLYIYVGGQNGYNGGGTGGQDGNSV
                     YSGPPGSLAASGGGASDVRLNGNALTDRVIVAGGGGGGGSNGVWPGCQVAGPAGNGGA
                     GGGTTGGNGTFGVGTPCNCGGGGGDGGLGGTSTAGGLAGGYYGSANCLRAGWTMGQDG
                     TLGQGGNGSLAYYNGTGGGGGGGGGYYGGGAGGNGSDTTPGGGGGGGSSYTGTLTSAS
                     TTAGSQSGDGMVVLTYIGTAPAVPSSITGPASVCTGSSVSYSISAVAGASGYTWAVPS
                     GSTITAGQGTTSITMTAGSVSGDVTVTADNSCGSSAAATYAITINALPVITAAASSPA
                     VCLGMNDTLTASGASTYAWSSGGTNATEVVAPSVNSTYTVIGTDMNGCNDTTMISVSV
                     NALPVVTASASSATVCSGTNDTLMVSGASAYAWSSGGTGATEIVAPSTNSTYTVIGTD
                     MNGCSDTTMISVSVNALPAVAASASSMAICSGAADTLTASGAIMYAWSSGGTNATEIV
                     MPSASTTYVVTGTDGNGCMNSDSVTVTVNPTPVITISGGPFTGCAPMCVQFVATGSPN
                     CVISQWNFGDSATSGNSSPMHCYTPGGIYSVIYTCTDANGCTGTATTTITAYAPPVAS
                     LTVSPSTYCVTDAPGLLSGTPSGGTYSGPGVTGSSFSPSAAGVGTQPVVYTYTDMNGC
                     SDTAMVPVTVNLCAGVDDISSPGALNIFPNPTNGVFTLAVNANEKNLLIEV"
BASE COUNT         1644 a         1909 c         2026 g         1481 t
ORIGIN      
        1 caagtgtgtg gctggtgacg aaggaagaaa aacggggaac agaagaagga acctggcgcg
       61 gcgcggtcga tgaacgaacg ggcacgttat ggctgggacg taatgagcac ctggaaaaaa
      121 atccgccgcc cgccggtgaa tactggaaaa atgttcctgt cacgcgtgcg ctgctccggc
      181 aggtgttcag cgatctttcc ttttcacaag catggctgct ggccggttac gggagcgtgc
      241 tcgcccggaa aaagttgtcg tggaacgctg gtgagttcgc aaaatgggaa caggaacgaa
      301 aagaaacgct ggcggaactt gctgcagatc tcgatccgga aacagcgata catgcgttcg
      361 ctgaaatgat gaaagatccg ggtgacgtaa aatacgacgc ggccgatttc gctgagctct
      421 tcggacaggt ggcaagacgc gatgaagata aaatgctttc tgtcctcgag agaacgttgc
      481 aggaaccggc cggcatggac agggatgaga ttgccctggc cctgggtgaa gccgggttgg
      541 aagaatggct gaagctggcg gatgaaaccg cggcagaaga tccgccggaa aattatgagg
      601 atgatatgga cgtgccgttg atctgcgcgg attgcttcgc cgggaaaccg gcctgcttcg
      661 atgcggtggg aaaaaagata ccgccgggca atcggcgcct ggaaagatac cacgcgcttc
      721 tccggaagct gtacgcagcc tgattttttc tttcgggaag acaacggcga aattaatttt
      781 tgcatcttca cgtaaacaaa ccacagcgga tgaaaaagat ctacactatt ttctcagtat
      841 gcatcgccct ctctgcttca gcgcagacca tcacctggaa ccagcagacc agcgccagca
      901 ccaatgatct ttacggggtt tcctttaccg acgcgaacca cggatggacg gccgggctcg
      961 gcggcgtgat acagaacaca acggatggcg ggaacaactg ggcgctccag aattcaggca
     1021 tcaccacgaa cctgcgtagc atatttttta tcgataacaa caacggctgg gcctgcggcg
     1081 atggcggcgt gatccttcac acatccaatg ccggcacttc atgggcggcg cagacgagca
     1141 ccacgacgaa caatcttcgc tccatccagt tcaccgatgc aaacaacggc tggtgcgccg
     1201 gaattgccgg taccatcctg cacaccggca acggcggaac cacgtggacc gcgcaaacca
     1261 gcaccagcac acagggactg atgcgcatcc atttcgccag caccactaca ggatgggtgg
     1321 cgggctcatc cgcaccgtcc acgagcggtt ttgcgctcag ctcaacgaac ggtggcggaa
     1381 cctggagcag ccagaccatc tctgcttcgt acaacttttt cggcctcgat tttgcgagcg
     1441 ccaccacggg atggatatgc ggaacgaatg gttccgtgta taaaaccacc aatggcggaa
     1501 cgagctggtc gcagcagacg acaggcatgt cgcacacgat gcgcggcgtt tctttcccca
     1561 cgctgaacga tggatggatg gtcggtgatt cagggtacat ctacattacc tccaatggcg
     1621 gtacgtcgtg gacacagcag cagacgtaca tgatcagctt tatgcgcgac gtgcatttcg
     1681 tgagcgtgaa cgccggctgg gccgtgggcg atcacggaac gatcctgcgc atcgtcgatc
     1741 cttccggcat cagcagtaaa gatcagttcg ctgatttcat ccgccttttc cccgatcctt
     1801 ccaatggaaa attcgaaatg tcggtaaccg taaaatcagc cacgacgctc agctggtctg
     1861 tcatggacat gagcggcagg acgatcatgc aggtgcggac ggatatttcc gccggcacga
     1921 caagcgttcc ggttgagctt cccgaaggaa cggacaaagg aatgtacatc ctcaccggtt
     1981 cgacggccgg cggcgagcag ttcgcggaga aattttccgt gcagtaagca attttctttt
     2041 acaggatatt ttcgtcgttg ccttacaggc gaccgttcct gctatcggag cgttggtcgt
     2101 tatttcgcat cgccgcatta ataaattgta actttgtaat tccgccggcg gatagaattg
     2161 aatcccggtt ccggtaaacg accaggatcg cggattggaa aaaaagggcc agcggaactt
     2221 accatgagtg acgccatcaa gcacgagtgc ggcatcgcgc tcatccgtct tctcaagccc
     2281 ctccagtatt atattgacaa atacggctcg ccgatgtacg gcgtcaacaa gctgtacctg
     2341 ctgatggaaa agcagcacaa ccgcggacag gacggggcgg gtgtggccac gatcaagttc
     2401 gatgttcctc ccgggacgcg ttacatcagc cgctacaggt ccacatcccc gacagcgatc
     2461 aaggatattt tcggaaagat cagcgacaaa ttttccgacg gttacaaaaa gaacgcggaa
     2521 aagatgaagg atgccacgtg ggtgaaacgt cacgtcgctt tttccggcga gctgctgctg
     2581 ggccatctcc gctacggaac gtatggcggg aacagcatcg agaactgcca tcctttcctg
     2641 cgccagaaca actggatcac acgcaacctc gtcgtggccg gtaatttcaa tctcaccaac
     2701 gtggatgaac ttttccatca cctcgtcgag ctcgggcagc acccgaaaga aaaagccgat
     2761 acggtgacgg tgatggaaaa gatcgggcat ttcctcgacc gtgaagtgga acgtcttttc
     2821 cagcattaca aagtgctggg acattcgaac caggagatca ccgatctcat cgcacgcgat
     2881 ctcgatatcc agaaaatcct gaaagacgcc ggcaaacgct gggacggagg ttacgccatg
     2941 gccgggcttc tcggtcacgg tgatgcgttc gtgctgcgcg accccaacgg tatccgtcct
     3001 gctttctggt gggcgaacga tgaagttgtt gtcgttgcgt ccgaacgccc gcagatcatg
     3061 acggcgttca atgcgcagct ggaagaaata aaagagatca cgccgggctc ggcgcttatc
     3121 atacgccgta acgggcatta tgaagaaaaa ggcgtacgca cgccccgtga aaaaaaatca
     3181 tgctcgttcg agcgcattta tttttcgcgc gggaacgaca aagatattta cgaagagcgg
     3241 aaaaatctcg gccgccagct gacgcccacc gtgctgaaat ccgttgcgtt cgacctgaag
     3301 aacacggttt tctcgttcat tccgaatacg gcggaagtcg ctttttacgg gctcgtggaa
     3361 ggactgcaca attacctcaa cacgatcaag cggcacaaga tccttacgat gaagaacgtc
     3421 gcggacccgg agctttccga aatcctcaac atgcagccgc gcgtcgagaa gatcgcgctc
     3481 aaggatgcga agctgcgcac gttcatcacc agcgacagcc agcgcgacga gatggtgtcg
     3541 cacgtgtacg atacgacgta cggcgttgtc aatcccggcg ataacctcgt tatgctcgat
     3601 gattcaattg tccgcggaac gacattgcgc cagagcatcg tcaagatcct cgatcgcctc
     3661 gctccgaaaa agatcgtcat cgtttcttct gctccgcaga tccgttatcc tgattgttac
     3721 ggtatcgaca tggcgcgcat gggtgatttc gtggcgttcc gcgcagcggt tgaattgctg
     3781 aaagacacgt accaggaaaa tatcctcgaa gaagtttacg aaaaagcgaa agcgcaggaa
     3841 gatcttcctg cgaacgaggt gaaaaatgtc gtgaaggaaa tttacaagcc gttcacggcg
     3901 gatgaaattt cacagaagat ttccgagctg ctcacgccgc ccgaggttag cgcgaacgtg
     3961 gaggtcatct accagagcat cgaaggtttg cacacggcgt gcccgggtca taccggcgac
     4021 tggtatttca ccggtgatta tcccacaccc ggcggaaaca aggtggtgaa ccgcgcgttc
     4081 atctacttca tggaaggaaa aaacgtgcgg gcgtactgaa gacagattat caccggaata
     4141 tccggaaagc caggaaatat ttgcggcagg aaactggttt cgggaataca cctttcagaa
     4201 atgacggcaa cggttgtctg ccgcgaaaaa tgatcctgcc gccttcctgc cgcctggaga
     4261 ttgtttctta ttttcagcag caaaataatc cctcaacctg ttaccatgtc acgtataagg
     4321 tttcgcccgg aacgtccctg gatcttccgc aggcccgcgt ttattgtttt cggaattgtc
     4381 ttcttcctgc tgctgatcct tgcgggactc gcgagcaacg agatgtataa taaatatatc
     4441 gaccgcaagt ggccacccga ctggtcgctg atcctgaaac cgctgtggca ggctccgatt
     4501 atcgccctgg tattttatgc gtgcgctgtg gctatctggt ttattctttg ggtgagaagc
     4561 cagggaggac gactattgta gttcacgatg caatgcagat gcttacgtga cccgttgccg
     4621 atagagtagg gggctttttc ttatattgtg ttcggcattt ttcaattcat aaactaaaat
     4681 tctctgcatg aagaacaaat tactcaccgc gatggcggtt ggtttgtccg tgtattccct
     4741 cgcgcagaca accaccacat tcaattacac cggggctgtt cagaccttta cggttccttc
     4801 ctgtgtcacc agtattacta tcgaagcgta tggcgcgcag ggaagcaatg cgacaacggg
     4861 cggtaccggc ggactcggcg gttatgctgt cgggacaatg aacgtcttgc ccggtgacgt
     4921 gctttacatt tacgtgggcg gacagaacgg ctataatggt ggcggtaccg gcgggcagga
     4981 tggaaattct gtttacagcg gtcctcccgg aagccttgct gccagcggtg gcggcgcttc
     5041 agatgttcgg ctcaatggaa atgcgctcac ggatcgcgtg atcgttgcgg gcggaggcgg
     5101 aggcggagga agcaacggcg tatggccggg gtgccaggtc gcgggtcctg ccggtaacgg
     5161 cggagccggg ggtggaacaa caggcggcaa cggaacattc ggggtgggca cgccctgtaa
     5221 ttgcggcggc ggtggcggcg acggcggtct cggcggaact tcaaccgcag gtggcctcgc
     5281 cggtgggtat tacggcagtg cgaactgtct ccgcgcagga tggacgatgg gccaggacgg
     5341 aacactcggg cagggcggaa acggctcgct cgcttattat aacggaaccg gcggcggcgg
     5401 cggcggtggt ggtggatatt acggtggcgg tgcaggtgga aacggatccg acacaacacc
     5461 cggcggcggt ggtggcggcg gttcctcata cacggggaca cttaccagcg ccagtaccac
     5521 ggccggttcg caatccggtg atggaatggt cgtgctgacc tacataggca cagcccccgc
     5581 agttccttct tcaattacag gccctgcctc cgtatgtaca ggctcttccg tttcctattc
     5641 gatctcagct gttgccggcg catcaggtta tacgtgggcc gttccttcgg gatcgacgat
     5701 cacagccggg cagggaacca cgagcattac gatgacggcc ggttctgttt ccggcgacgt
     5761 aacggtaaca gctgacaaca gctgtggaag cagcgccgca gcaacgtatg cgatcacgat
     5821 caacgcgctc ccggttatca ctgccgcagc ttcttctcct gccgtatgtc ttggaatgaa
     5881 cgacacgctc acggcttccg gcgcatccac atacgcatgg agctcgggcg gaacgaatgc
     5941 aacggaagtt gtcgcaccgt cggtgaattc cacgtacacc gttatcggca cggacatgaa
     6001 cggatgcaac gacacgacga tgatctctgt ttccgtgaat gcgcttcccg tggtcacggc
     6061 atcagcatct tctgcaacgg tatgcagcgg aacgaatgat acgctcatgg tttccggcgc
     6121 atctgcttat gcgtggagct ccggcggaac aggtgcaacg gagatcgttg cgccatcaac
     6181 gaattccacg tacaccgtca tcggcacgga catgaacgga tgcagcgata cgacgatgat
     6241 ctctgtttct gtgaatgcgc ttcctgcagt cgcggcttcc gcatcttcaa tggcgatctg
     6301 ctccggcgca gccgacacgc tgacggcaag cggtgcgatc atgtacgcat ggagctccgg
     6361 cggaacgaac gcgacggaaa tcgtcatgcc gtctgcaagc acaacgtacg tcgttaccgg
     6421 aacggacggc aacggttgca tgaattcgga ttcggtgacg gtaacggtga atccgacacc
     6481 ggtgatcacc atctccggcg gaccgttcac aggttgcgcc ccgatgtgcg ttcagtttgt
     6541 cgcaacggga agtccgaact gcgtgatctc gcaatggaat ttcggagact ccgcaacgag
     6601 cggcaattcc agcccgatgc attgctacac gccgggagga atatacagcg tgatctacac
     6661 ctgcaccgat gcaaacggat gcacgggcac tgcaacaacg acgatcaccg cgtatgctcc
     6721 gcctgtcgca tcgcttaccg tttcaccttc gacttattgc gtgaccgatg ctcccggact
     6781 tctttcaggc acgccttcgg gcggaacgta ttcgggtccg ggcgtgacag gaagttcctt
     6841 ctctccttca gcggcgggag tgggcacaca gccggttgtc tatacctaca cggacatgaa
     6901 cgggtgcagc gatacggcaa tggttcctgt tacggtgaat ctttgcgccg gcgtcgatga
     6961 tatttcatcg ccgggagcgc tgaacatttt cccgaatccc acgaatggcg tgtttaccct
     7021 cgccgttaac gccaatgaaa aaaacctgct gatcgaagtt
//
LOCUS       DASTSF010000513         4565 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1881214,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000513 DASTSF010000000
VERSION     DASTSF010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4565
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1881214"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            172..891
                     /locus_tag="VFU15_14650"
     CDS             172..891
                     /locus_tag="VFU15_14650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719080.1"
                     /translation="MRVSVISFFLLLAVLVPAKTKAQNFTYPVIRKTAASLNAFVPPG
                     WEILDSATGDLNKDSADDVAFILQHRDSVPLTEIISRDTETVVTQPRILVIAFRDVKK
                     KKYTVQEQSNTFILCHDQENMSDPYVSMRISGGVLQLDFQLFCNMGCWDVTNLSYKFR
                     FQENEFVLIGADYSTMNRASLDFDTRSYNFLTKKWSRSTGSDKYNTHKTEWLTLDVPA
                     MKTLRTFTRPLTWEVTKDLYL"
     gene            996..2696
                     /locus_tag="VFU15_14655"
     CDS             996..2696
                     /locus_tag="VFU15_14655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719081.1"
                     /translation="MKRLLTALLLVATALNVSAFTFYDQLCTFNFNWKKYAMQAPAGE
                     ARNFHSDAEYVQAHLGAVLNILRAEPVDHLSDAQKISRAHLLDLLDGYRAAGKFPVNY
                     YKPYRVPVFIDDNGTHCAVSYLMQQTGNETMAERIAQTDNFAWVKDIRDPGVPSWQEA
                     SGLSVDDLKLIQGAYDFYLPDAFTSPDKYATPQKPACITAYFTDNITGKSLPEKPGNI
                     WCKGEGKDGVLNGKWIQNYAAGMPWIEGFFTNGKRSGQWKEYYQGTDKLCRTEHWSNN
                     KLNGTRERYDLSGNLIEEITFSNGNAISKINYDVNDSVAYVRVPIDSIHVWTQVFNAS
                     GTLIACGHETVFNPGGLLWFQNIELTALNSASITARSVVQNEANGSPGATGGYYGNNL
                     YNNPPLVQYLKQGDWVYYHDNSALRRDMYSLPYGAKLLCEKFPHFGTELWTSIFLFPH
                     ASISNNYDSIHVVYKNDRVLHFYGYSKTDFLHLYAEYYDSVPALYPVYARRGILSGFG
                     QDYPVQLSPGDQMRVKKTGQYTRDGKRTGEWKFYSSPGVLYKTEDYLVPTDEKEAPEQ
                     "
     gene            2821..3789
                     /gene="hemB"
                     /locus_tag="VFU15_14660"
     CDS             2821..3789
                     /gene="hemB"
                     /locus_tag="VFU15_14660"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014681063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEU4719082.1"
                     /translation="MLQRPRRTRKNAIIRNLVAETRLSKEQFIYPYFVTHGKNVVHPI
                     AAMPGISHFSVDTLLKDAEKGLKHGINKLLLFGVGEEKTEDGSSSHSGHSIVPQAVAA
                     LKKNFGDDIYVITDVCVCAYTTHGHCGILHDGYVENDASVELLSKMALSHAQAGADMV
                     APSDMMDGRVGAIRRTLDENGFVNTALMSYAVKFASAYYGPFREAADSSPKSGDRKAY
                     QMDARNANETIREALLDVEEGADIIMVKPALAYLDVIRRVKENILLPVSCYNVSGEYS
                     MVKIAAANGLIDEQKIVMENMTAFTRAGADIIITYHARDIVEKGWL"
     gene            complement(3818..4444)
                     /locus_tag="VFU15_14665"
     CDS             complement(3818..4444)
                     /locus_tag="VFU15_14665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719083.1"
                     /translation="MVIGTQFFGKVRKVNGQAIATKFVIIGVPLFPVTTMFVVKDMGN
                     TKQGYEIDLNGESVGLGYGRMITFILGLILTLIGSIGIHEERLLVPGMICIGLWGVFQ
                     FVLGQPRKEEEAKRLLIGNILGVNAFPEWLPQHVSNALYRQLQALYEQRKDKYGGMTW
                     EQCIKGRRAIQPDYALFFTLAVYRNYFMPGMESEKLLEDVWSMRQTVS"
BASE COUNT         1148 a         1273 c         1154 g          990 t
ORIGIN      
        1 gacaagatcg acacggagct ggcggggctt gaacaatacc gttggaaaaa caaagcgttt
       61 tcgtattcag gaaaataatc cgggtttcaa cgcggtaaag tccaaccgcg gaacgacgaa
      121 cggaaaacca ttgaaacccg gaactgttca aacccggaaa ccattctgat tatgcgcgtc
      181 agcgtgatct cattttttct tttgctcgcc gtgcttgttc cggctaaaac aaaagcgcag
      241 aatttcacgt atcccgtcat tcgcaaaaca gccgcttcgc tgaatgcgtt cgttcctccc
      301 ggctgggaaa ttctcgattc cgccaccggc gacctcaata aagattccgc cgatgacgtc
      361 gcattcattc tgcagcacag ggacagcgtc ccgctcacgg aaataatttc ccgagatacg
      421 gaaaccgtcg ttacgcagcc gcgtatcctc gtgatcgctt tccgggacgt aaaaaagaaa
      481 aagtacaccg tgcaggaaca aagcaatacg ttcattcttt gccacgacca ggaaaacatg
      541 agcgatccgt atgtttccat gcggatcagc ggcggcgtat tgcagctcga tttccagctg
      601 ttctgcaaca tgggctgctg ggacgtcacc aacctttcgt acaaattccg tttccaggag
      661 aatgaattcg ttctcatcgg tgcggattat tcaaccatga accgcgcatc gcttgatttc
      721 gacacccgca gctacaactt cctgacgaag aaatggagcc gttcgacggg aagcgataaa
      781 tacaatacgc acaaaacaga atggctcaca cttgacgtgc ccgcgatgaa aacgctccgg
      841 acgttcacac gtccgctgac ctgggaagtg acgaaagacc tttacctgtg aatttttgaa
      901 acgaacaggc ccgtcgttga acttttcatc ttatggcgtg tactaaatgg tccggcagga
      961 aaagctcttc ccggcaactc atttaaacac gaaccatgaa acggctgctt acagctcttc
     1021 tgctggtcgc aacggcgctg aatgtttccg cgtttacgtt ttacgatcag ctctgcacat
     1081 tcaacttcaa ctggaaaaaa tacgccatgc aggcgcccgc aggtgaggcg cggaattttc
     1141 attccgatgc ggaatatgtg caggcgcacc tcggcgcggt gctgaacatc ctgcgtgccg
     1201 aaccggtgga ccatctcagt gacgcgcaga aaatctcgcg cgcgcacctg ctcgatcttc
     1261 tcgacggata ccgcgccgcg gggaaattcc cggtcaacta ttacaagcct tatcgcgtcc
     1321 ccgttttcat cgacgacaac ggaacgcatt gcgccgtgag ctacctcatg cagcagaccg
     1381 gcaacgaaac gatggcggaa cgcatcgcgc agaccgacaa cttcgcgtgg gtgaaagaca
     1441 tccgcgatcc cggcgtgcct tcgtggcagg aagcgtcggg cctttccgtc gacgacctga
     1501 agctgatcca gggcgcgtat gatttttacc tgccggatgc gttcaccagc ccggacaaat
     1561 acgcgacgcc gcagaaaccc gcgtgtatta ccgcctattt cacggacaat atcacgggaa
     1621 aaagtttgcc cgaaaaaccg ggaaacatct ggtgcaaagg cgaaggaaag gacggcgtgc
     1681 tcaacggaaa atggatacag aattacgctg ccggcatgcc gtggatcgaa ggttttttta
     1741 cgaacggaaa acgctcggga caatggaaag aatattacca gggaacagac aagctctgcc
     1801 gcacggaaca ctggagcaat aacaagctga acggaacacg cgagcgttac gacctgtccg
     1861 gaaaccttat cgaagaaata acgttctcga acggcaatgc catttcgaag atcaattacg
     1921 acgtgaacga ctccgtcgcg tacgtgcgcg tcccgatcga ttccatccat gtctggacgc
     1981 aggtgttcaa tgcttccgga acgctcatcg cgtgcgggca cgaaactgtt ttcaatcccg
     2041 gcggactgct ctggttccag aacatcgaac tcaccgcgct gaattccgct tccatcaccg
     2101 cgcgttccgt ggtgcagaat gaagccaacg gttccccggg tgcgaccggc ggttattacg
     2161 gaaataatct ttacaacaac ccgccgctgg tgcaatacct gaagcagggc gactgggtgt
     2221 attaccacga taattccgcg ctgcgccggg acatgtactc actgccgtat ggcgccaagc
     2281 tgctctgtga aaaattccct catttcggga ccgagctctg gacctccatt ttcctcttcc
     2341 cgcacgcttc gatcagcaac aactatgatt ccatccatgt cgtatacaaa aacgatcgcg
     2401 tgcttcattt ttacggctac agcaaaactg attttctcca cctgtatgca gaatattatg
     2461 acagcgttcc ggcgttgtac ccggtctatg cccggcgcgg aattttgtcg ggtttcggcc
     2521 aggactatcc cgttcaactt tcaccgggcg accagatgcg ggtcaagaaa acgggacaat
     2581 acacacgcga cggaaaacgg accggggaat ggaaattcta ttcttcccct ggcgtgctct
     2641 ataaaaccga ggactacctg gtcccgacgg atgaaaaaga agcacccgaa caataaaaac
     2701 gtttttttaa ccccgcgaca aaaccccgta aaaccgcaag gcatcacggg gtttttcatt
     2761 cccgcttttt ctgacaattc ccggacatac tttgattcac ctgcgcgtac ctttgcgtcc
     2821 atgttacaac gtccgcggcg cacgcgtaaa aatgccatca tccgcaacct cgtcgctgaa
     2881 acgcgattga gcaaagagca gttcatttac ccgtatttcg tcacgcacgg aaaaaatgtc
     2941 gttcacccga tcgcagcgat gcccggcatc agtcattttt ccgtcgacac attattgaaa
     3001 gacgcggaaa aaggattgaa gcacggcatc aacaagctgc tgctgttcgg cgtgggcgaa
     3061 gaaaaaacgg aagacggcag ctcctcgcac agcggtcaca gcatcgtccc gcaggcagtg
     3121 gctgcgctga agaaaaattt cggcgacgac atttacgtca tcaccgacgt gtgcgtatgc
     3181 gcctacacca cgcacgggca ttgcggaatt ctccatgacg gctacgtgga aaacgatgca
     3241 tccgtggagc tgctttctaa aatggcgctc tcgcacgcgc aggccggcgc agacatggta
     3301 gcgccttccg acatgatgga cgggcgtgtg ggcgcgatcc gcagaacgct cgatgaaaac
     3361 ggttttgtga atacagcgct catgtcgtac gcggtgaaat tcgcttcggc gtattacggt
     3421 ccgttccgcg aagccgccga ttcatccccg aagtcaggcg accgcaaagc ctaccagatg
     3481 gacgcgcgca acgcgaatga aacaatacgt gaagcgctgc tcgacgtgga agaaggcgcc
     3541 gacatcatca tggtgaaacc ggcgcttgcg tatctcgatg tcatccgccg cgtgaaggaa
     3601 aatatcctgc ttcctgtttc ctgctacaac gtctccggtg aatattccat ggtaaaaatt
     3661 gcagccgcga acgggctgat cgacgaacag aagatcgtga tggagaacat gacggcattt
     3721 acccgcgcag gagcggacat catcatcacg taccatgcgc gggacattgt ggagaaagga
     3781 tggctgtaaa tcacagtttc ctcaacggaa ccggtgatta cgatactgtc tgccgcatcg
     3841 accacacatc ttccagcagc ttttccgatt ccatgcccgg catgaaatag ttgcggtaca
     3901 cggcgagcgt gaagaaaagc gcataatccg gttgtatcgc acgccgtccc ttgatgcact
     3961 gttcccaggt catgccgccg tatttgtcct tgcgctgttc gtacaaggcc tgcagctggc
     4021 ggtaaagcgc attcgaaaca tgctgcggca gccattcggg aaacgcattc acgccgagaa
     4081 tgttcccgat gagcaaacgt ttcgcctcct cctcttttct cggctggccc agcacgaact
     4141 ggaaaacgcc ccacagcccg atgcagatca tgcccgggac gagcaggcgc tcttcatgaa
     4201 tcccgatcga tccgatcagc gtaaggataa gtccgagaat gaatgtgatc atcctgccgt
     4261 acccgagacc aacgctttcc ccgttgaggt cgatctcgta accttgcttt gtgttgccca
     4321 tgtctttcac cacgaacatc gtcgtgacgg gaaacaaggg tacgccgatg attacgaatt
     4381 ttgtagcgat ggcttgtccg ttcactttgc ggacctttcc gaaaaattgt gtgccgatga
     4441 ccattgggat tgggggttgg gttggtgaaa atatcaaaaa cttttgaccg aaaaaaccgg
     4501 ccgtaaacgt gtggcaattg actgcgttca cattccggca cgatttttac atagctttat
     4561 ccgac
//
LOCUS       DASTSF010000514         2017 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_680556,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000514 DASTSF010000000
VERSION     DASTSF010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2017
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_680556"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2017)
                     /locus_tag="VFU15_14670"
     CDS             complement(<1..>2017)
                     /locus_tag="VFU15_14670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719084.1"
                     /translation="IDSSGDSLELIIQQDRLNGTQSVFTQHRMATHYEYNSLNQLVRQ
                     SMPDQDNMDIWQTTLPNGLDSRFKAASSQFVSATRGYLSGYIDAGSGITRGTVYITDD
                     GGSTWKRMGGEVGADINKIQWVSTSVAYAVGDYGTVIKSSDGGNNWDMLSTWSVNLTG
                     GALNTSNLNDLYFTDATHGVVVGDDGLMLKTTTGTSFTQIDLITGGTLMAGDDITSVT
                     YDGTDYYITVFRPGTSSPDEGLIFKSPDLTTWTQQTNVRTIADMKKVQYYDDASAYAA
                     GEDGTLLHTADNGASWQLVHTSVTGEFRDIFFRNENEGIAILTNSVGDGELWKTFDKG
                     VTWTLLGNADEDYQALYPYENNSGGAKVVAVGSNGLVSRVIMTANTPFGVIPLNSPAS
                     TTFHASWATKVGSDLWLYVAGADGNVYYTKDATASYLTWVTASTGMSGEYPVKMSAMQ
                     TSSGTDPGINGTLITNTGELWSLHKVQNSSTYSFAVFSSPATGSSNFADITSAGSDSA
                     LVAFNNYDQKLYSIVLTNTAATSTASTVGSGTASYSTIVSLATDTGDAIISTSGGELE
                     YIAYTISSPSTTFTDRTEKITSLPLHAVEAASGGNVYAVGDDGTFIEQNTSDHWRIMV
                     SGIADNINATAFSSATVGLIAGDAGSVYNFSITGSSMIMHARTSGTTE"
BASE COUNT          383 a          541 c          615 g          478 t
ORIGIN      
        1 ttccgtcgtt ccgctcgtgc gggcgtgcat gatcatggag cttcccgtga tggaaaagtt
       61 gtaaacactg cctgcgtcac cggctatgag gcccacggtg gcggagctga aggctgtggc
      121 gttgatgttg tccgcaatcc cggaaaccat gatgcgccag tggtccgatg tgttctgctc
      181 gatgaacgtc ccgtcgtcgc cgacggcata cacgttcccc ccgctcgctg cttccacggc
      241 gtgcagcgga aggctggtga tcttttcggt gcggtcggtg aatgttgtcg aaggagaaga
      301 gatcgtgtaa gcgatgtatt cgagctcgcc gccgctggtg gagatgatcg cgtcgcccgt
      361 atcggttgca agggaaacga tcgtcgagta gctggccgtg ccggagccga ccgtggaagc
      421 ggtggatgtg gctgcggtgt tcgtcagaac gatggaatat aatttctggt cgtaattgtt
      481 gaatgcgacc agcgcagagt ccgaaccggc ggaagtgatg tccgcgaaat tgctcgagcc
      541 ggtggccgga gaagagaaca ccgcgaagga atacgtgctg ctgttctgca ccttgtgcag
      601 gctccacaat tctcctgtat tcgtgatgag cgttccgttg atgccgggat ccgttccgga
      661 agaagtttgc atagcgctca tcttcacggg atattccccg ctcatgccgg tggaagccgt
      721 cacccaggtg aggtagctcg ccgtcgcgtc tttcgtgtaa tagacgtttc cgtccgcgcc
      781 ggcgacatac agccagagat cgcttcccac tttcgtcgcc cacgatgcgt ggaatgtagt
      841 cgatgcagga gagttaagcg ggatgacgcc gaaaggcgta ttcgcggtca tgatcacgcg
      901 actcacaagg ccgttcgacc cgacagccac gaccttcgcg ccgccggaat tattttcata
      961 cgggtagagc gcctggtaat cttcgtctgc gttgccgagc aaagtccacg ttacgccttt
     1021 gtcgaacgtt ttccagagct ctccgtcccc gacgctgttg gtcaaaatgg cgatgccttc
     1081 attttcattg cggaaaaaga tatcgcggaa ttctcccgta acggaagtat gcacgagctg
     1141 ccacgaagcg ccattgtccg cagtatgcag cagcgttccg tcttcgccgg ccgcatacgc
     1201 ggaagcgtcg tcgtaatact gcactttttt catgtcggcg atggtgcgta cgttggtctg
     1261 ctgcgtccag gtggtgagat ccgggctttt gaaaatgagc ccttcgtcgg gagaagaagt
     1321 tccgggacgg aaaacggtaa tgtaataatc cgtgccgtcg taggtgacgg aagtgatgtc
     1381 gtcgccggcc atcagcgttc cgccggtgat gagatcgatt tgcgtgaagg aagttccggt
     1441 cgtggttttc agcatcagcc cgtcgtcgcc gacgaccacg ccgtgcgttg cgtccgtaaa
     1501 gtacaggtcg ttgaggttcg atgtattcag cgcgccgccc gtaagattga cggaccatgt
     1561 gctgagcatg tcccagttgt tgccgccgtc ggaggattta atgacggtac cgtaatcacc
     1621 taccgcgtaa gcgaccgaag tgcttaccca ctggatcttg ttgatgtcgg cgcccacttc
     1681 tccacccatg cgcttccacg tacttccccc gtcgtcggtg atgtaaaccg ttccacgtgt
     1741 aatgccgctt ccggcgtcta tgtagccgct caggtagcca cgcgtggcgg aaacgaactg
     1801 gctgcttgcc gctttgaagc gcgagtcgag cccgttcggc agcgtggttt gccagatgtc
     1861 catgttatcc tggtcgggca tggattgtcg caccaactgg ttgaggctgt tgtattcgta
     1921 atgcgtggcc atgcggtgct gcgtgaatac gctttgcgtt ccgttgaggc ggtcctgctg
     1981 aatgataagc tcgagcgaat caccggaaga atcgata
//
LOCUS       DASTSF010000515         6306 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1435573,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000515 DASTSF010000000
VERSION     DASTSF010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 6306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6306
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1435573"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            105..623
                     /locus_tag="VFU15_14675"
     CDS             105..623
                     /locus_tag="VFU15_14675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719085.1"
                     /translation="MTPQEILSQKDVKPKEKTEMISRMLSEKKFSAGEWLELASAAKD
                     PVKATLIEAMEFSTKEVPALLDEPGFDFLVRSLSAKAPRVKWESAKTIGNTAQRFPHK
                     LDEAVEALKKNAADEGTVVRWSAAFALGEIVKLNLPLNKQLVPLVKKIMEKEEKNSIR
                     KIYAAALKKAAG"
     gene            816..1424
                     /locus_tag="VFU15_14680"
     CDS             816..1424
                     /locus_tag="VFU15_14680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719086.1"
                     /translation="MLAGNDGNDSMRRPAFLIKASPLPLVYAIYPEGSSDLGFEMRIA
                     PHFTVGINGGVAYSNPFFRFSSHQHGYFFSGEFRYYFPKRNGTYLSFSPAYNCIFFSP
                     NVDFKLANGEYLKEVSYTEQTNAFCVYFGMEGNGEKSRWPLDFSIGAGAGYRYGNAPG
                     LNADEINFIDHNSIAGLKGDKTGWHVVPVVNINARICFIIRD"
     gene            1470..1805
                     /locus_tag="VFU15_14685"
     CDS             1470..1805
                     /locus_tag="VFU15_14685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719087.1"
                     /translation="MNEEQQPAPRLLWIIVCAVLSFLFFFLAIALNAFRYQLFGINAH
                     DAPGNFSFNTAIFIPLVLGSILLIPFVIYGVIAKWKYKPRRRLLLVSFLFILPELTFL
                     VIGLFLLFR"
     gene            complement(1836..2732)
                     /locus_tag="VFU15_14690"
     CDS             complement(1836..2732)
                     /locus_tag="VFU15_14690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719088.1"
                     /translation="MLATGFWFLHALSKEYVITVRIPVAYSHLPEKGLVAVDLPDSVD
                     AEVSGSGFSLFAYQWLSGTEPLPLDFRQSRSVGGGNFALATLQHPDRVEGVVGHGLKV
                     LSVMPDTIILNFAGRVEKKVPVRPMVTVQCAPLCRMGDSIRTYPQFVTISGAEALVDR
                     VSYLETETKTYRNVDKAVNEAIGLKIPPDLSQLIVAPSQVNLVIPVGKYTEKRVSVPV
                     ETINVPANSMVKTFPDKVDIVFEVPVGDYQNVSASMFRVVADYSKAKPGDKYLQLEIV
                     RRPLNILNPRIEPQKVDYLIRK"
     gene            complement(2862..3239)
                     /gene="yajC"
                     /locus_tag="VFU15_14695"
     CDS             complement(2862..3239)
                     /gene="yajC"
                     /locus_tag="VFU15_14695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008243997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEU4719089.1"
                     /translation="MHLLHFLLDAAPAQGQKGGSSLLDPGMLLMIGGIALVFYFFMYK
                     PQKNKQKEAETFQEGLVRGTKVVTIGGIIGKILEVRNKTFVIEAEGGTKLQVLKTHVS
                     LESTKALNNEGKDEKNGDKKDDK"
     gene            complement(3259..3738)
                     /locus_tag="VFU15_14700"
     CDS             complement(3259..3738)
                     /locus_tag="VFU15_14700"
                     /inference="COORDINATES: protein motif:HMM:NF019242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HEU4719090.1"
                     /translation="MKSTILSVFALALVFSFTSCAPKKNKEVTTDLVDITSTASGNAP
                     AGKAPVMKFEVEEHDFGTISQGERVSFPFKFRNVGGSDLIISEAHGSCGCTVPDYPTK
                     PIAPGQDAVVNVEFNSEGKRGKQEKTVTMTTNCEPNTQVITIHANVVVPADRGGDSK"
     gene            complement(3805..4755)
                     /gene="nusB"
                     /locus_tag="VFU15_14705"
     CDS             complement(3805..4755)
                     /gene="nusB"
                     /locus_tag="VFU15_14705"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEU4719091.1"
                     /translation="MLNRRYLRIKALQALYGYFQSEDGDMARGERELMRSIDRTYELY
                     IYLLLVFGEIARVARNQIDLGREKMLPTQEDLNPNTRFVSNRLIGQFSGLAALNKEAA
                     ARKLTWQSDAEISMLRKMWEQVKSWDVYREYMNNPDSTYETDREFVLDFFKKFIADDD
                     STEQFLEEKNMHWPGDLNMAVAPTIIRTIENASESREPQLAPLFKDPEDDKQFVLDLY
                     RKTILDNQKSEKLISEKTRNWEVERIALMDVLLMKMAITELQHFSSIPVKVTLNEYIE
                     ISKTYSTPRSKQFINGILDKLVADFRTDGSMKKTGRGLME"
     gene            complement(4788..5894)
                     /locus_tag="VFU15_14710"
     CDS             complement(4788..5894)
                     /locus_tag="VFU15_14710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013188325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HEU4719092.1"
                     /translation="MLTANDPKTSSVAAGPLSQMSTFDHEQVVFCNDNATGLRSIIAV
                     HNTVLGPALGGTRMWPYATEAEALTDVLRLSRGMTYKAAITGLNLGGGKAVIIADSRK
                     DKNEMLMRRFGKFVNSLGGKYITAEDVGINSRDMEYVFMETKHVTGIPPALGGSGDPS
                     PVTAYGVYMGMKAAAKERWGNDSLSGKKIAVQGVGHVGSNLVAHLVREGAKVFITDIY
                     EDRVADVKKNNSGVEFVAGDAFFDLDIDVYAPCALGATLNDENLARLKCSIVCGAANN
                     QLADENVHGKIVMEKGILYAPDYVVNAGGLINVYSELAGYGREYAMSQAENIFNTTSS
                     IFGASKKENIPTFMAANRMAEARIEAVAKIRTKR"
     gene            6021..>6306
                     /locus_tag="VFU15_14715"
     CDS             6021..>6306
                     /locus_tag="VFU15_14715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HEU4719093.1"
                     /translation="MKALGYLNKYFWKYRLRFFSGILFIIISTFFLTRPVELLGKGID
                     FAINANRFHYPSGPTAHVLLLYGLEIISDTLIYGFFLFLNRQTIIVMSRLI"
BASE COUNT         1361 a         1634 c         1617 g         1694 t
ORIGIN      
        1 cattcaatga cagcggcacg ctcacacgcg gacctgccgc gcgaagcctg cgcatgtaca
       61 acacatctct cagcgggaac agtctccgca tttattcata acacatgaca ccacaagaaa
      121 ttctttcgca gaaagacgtt aagccaaaag aaaaaacgga aatgatcagc cggatgcttt
      181 cggaaaagaa attttccgcc ggcgaatggc tggagctcgc ttcggcagca aaggatcccg
      241 tcaaagcgac gctgatcgaa gcgatggaat tttcaacgaa ggaagtccct gcattgctgg
      301 atgagcccgg ctttgacttc ctcgtccgtt cgctttccgc aaaagcgccg cgcgtaaaat
      361 gggaatcggc aaaaacgatc gggaacacgg cgcagcgttt cccgcacaag ttggatgaag
      421 cggtggaggc cttgaaaaag aatgcggcgg atgaaggaac cgtggtccgc tggagcgctg
      481 cgtttgcact cggggagatc gtgaagctga accttccgct caataagcag cttgtgcccc
      541 tggtgaaaaa gatcatggaa aaggaagaaa agaacagcat ccggaaaata tatgccgcgg
      601 cattgaaaaa agcggccggc tgatacagct tccgttgaaa cgcagtaaga aaaaataagg
      661 gaagttgatg acggtcatta tttttccagc ggtaaggacg gatttcatgg agtaatgtca
      721 taggcgttgg ttttcgcttt ccggtaattt gtaaaatgat tttacgcgga aaattcattg
      781 cacttttaat gggaataatg ctggtgtcgg caaagatgtt ggcagggaat gatggaaacg
      841 attccatgcg gcgcccggca tttcttatta aagcttcgcc cctgccgttg gtttatgcta
      901 tttacccgga aggaagcagc gaccttggtt ttgagatgag gatagctccg catttcaccg
      961 tcggcattaa tggcggagtg gcgtacagta atcctttttt tcgcttctcg tcgcatcagc
     1021 atggctattt tttcagcggc gagttccggt attatttccc gaaaaggaac ggaacttatc
     1081 tttcattttc cccggcttac aactgcatat tcttttcgcc gaacgtggat ttcaaattgg
     1141 cgaacggcga atacctgaaa gaggtttcct atacggaaca gacaaatgct ttttgcgtgt
     1201 atttcggtat ggaaggaaac ggggagaaaa gccgctggcc gctcgatttt tcaatcggcg
     1261 ccggcgctgg ttatcgttat ggaaatgcac cgggtcttaa tgcggatgag atcaatttca
     1321 tcgatcataa ttccattgcc gggctgaagg gagacaaaac cgggtggcac gtggttcctg
     1381 tagtaaatat caatgcgcgg atctgtttta ttatccgcga ttgaacctgc catgtttttt
     1441 ttcgcaactt gctgttaaat ccgttcgtta tgaatgaaga acagcaaccc gctccgcgcc
     1501 ttttatggat aattgtctgc gcggtacttt ccttcctgtt tttcttcctc gccattgcgc
     1561 tgaatgcttt ccgctaccag ttgttcggga tcaatgcaca tgatgcgcct ggaaattttt
     1621 cgttcaatac agcaattttt atcccgcttg tgctcggctc catcctgctg atcccgtttg
     1681 tgatttacgg tgttattgcc aaatggaaat ataagccccg gcgcaggctg ctgctggtct
     1741 ctttcctgtt tattttaccc gagctgacct tcctcgtcat cggtctcttc cttctttttc
     1801 gctgagaaaa acagtgcgcg gaacgcgggg agaatttatt tccggatgag gtaatccact
     1861 ttctgcggct cgatcctcgg gttgagaata ttgagcggac gacgcacgat ctccagctga
     1921 agatacttgt cgccgggctt cgcctttgaa taatcggcaa cgacgcggaa catggaagcc
     1981 gaaacattct ggtaatcgcc gaccgggact tcgaacacga tatccacttt gtcgggaaaa
     2041 gttttgacca tgctgttcgc cggaacattg atcgtctcca cgggaacgct cactcttttt
     2101 tccgtgtatt tccctacggg aatgaccagg ttcacctgtg aaggcgcaac gatgagctgg
     2161 gaaagatcag gcgggatctt caggccgatc gcttcattca cggccttgtc gacgttcctg
     2221 taggtctttg tttccgtttc gaggtagctg accctgtcga cgagcgcttc cgctccgctg
     2281 atcgttacga actgcggata agtgcggatg ctgtcgccca tccggcacaa aggcgcgcac
     2341 tgaacggtaa ccattggcct cacgggaact tttttctcga ctcttcctgc gaaattcagg
     2401 atgatcgtat caggcatcac gctgagcact ttcagtccgt gtcccacgac accttctacc
     2461 ctgtcgggat gctgcagcgt ggcgagcgca aaatttccgc cgcccactga acgcgattgc
     2521 cggaaatcaa gcggcagcgg ctccgttccg ctcagccatt gataggcaaa aagggagaac
     2581 ccgcttccgc tcacttccgc atcgacagaa tcgggaaggt caacggctac cagtcctttt
     2641 tccgggagat gcgaataggc caccgggatg cgaacggtga tgacatattc tttcgaaagc
     2701 gcgtgcagga accagaaccc ggttgccagc aatatgcaaa tgagaaatgt gaatccgcgg
     2761 cggttgcgga atgggatttt ttcgccgggt tgagtgaatt ttatcgggtc cttttcagac
     2821 aagcgcgttg ggtcattaca atgcggaaaa gcgtctgcgg gttatttatc gtctttcttg
     2881 tccccgttct tctcgtcttt tccttcgttg ttcagcgcct tggtgctttc aagggaaaca
     2941 tgcgttttca gcacctgcag ctttgttccg ccttcagcct cgatcacgaa cgttttgttg
     3001 cggacttcga ggatcttccc gataatgccg ccgatcgtca ccactttcgt tccgcgcaca
     3061 aggccttcct ggaaagtttc agcttccttc tgcttgttct tttgcggctt gtacatgaaa
     3121 aaataaaata cgagcgcaat tccgccgatc atcagcagca ttcccggatc aagaagcgag
     3181 ctcccgcctt tctgtccctg tgccggtgct gcgtccagca gaaaatgtaa tagatgcata
     3241 gtgatttgtt gttagaaatt atttgctgtc accgccacgg tcggcaggaa cgacaacgtt
     3301 tgcatgaata gtaatgacct gtgtgttcgg ctcgcagttg gtcgtcatcg tgacggtctt
     3361 ttcctgcttg ccgcgctttc cttcactgtt gaattccacg ttcaccaccg catcctgtcc
     3421 gggagcaatg ggcttcgtgg gataatcggg aaccgtgcag ccgcagcttc cgtgggcttc
     3481 ggagataatg agatcacttc cgccgacgtt cctgaattta aacgggaagc tgacgcgttc
     3541 tccctgcgaa atggtgccga aatcgtgctc ttccacttcg aatttcatca caggcgcttt
     3601 gcctgcgggc gcatttccgc tggccgtcga agtaatgtcg acgagatcag ttgtcacttc
     3661 cttgttcttt ttgggcgcac aggaggtgaa cgagaaaacg agcgccaggg cgaaaacgga
     3721 aagaattgtg cttttcattt tccttttttt tagctgtgac aaaattaaac ttttcacgca
     3781 gcagcgggaa taaataattg cgaattattc catcagcccg cggcctgttt ttttcatgga
     3841 gccgtcggtg cggaaatccg ccacgagctt gtcgagaatg ccgttgatga attgcttcga
     3901 acgcggcgtg ctgtaggttt tcgagatctc aatgtattca ttgagcgtca ccttcaccgg
     3961 gatgctggaa aaatgctgca gctccgtgat cgccattttc atcagcagga catccatcag
     4021 cgcaatgcgt tcgacttccc agttgcgcgt tttttcggag ataagctttt cgcttttctg
     4081 attgtcgaga atggttttgc ggtaaagatc gagcacgaac tgtttgtcgt cctccggatc
     4141 tttgaacagc ggcgccagct gcggctcccg gctttccgat gcgttttcta tcgtacggat
     4201 gatcgtcggg gcgaccgcca tgttgaggtc cccgggccag tgcatgttct tctcttcgag
     4261 gaactgctcc gtcgagtcgt cgtcggcaat gaattttttg aagaagtcca gcacgaattc
     4321 cctgtccgtc tcatacgtgg aatcgggatt gttcatgtat tcccggtaaa catcccagct
     4381 tttcacctgt tcccacatct tgcgcagcat gctgatctcg gcatccgatt gccaggtgag
     4441 tttccgggcc gccgcttctt tgttcagcgc agcaagaccg gagaactggc cgatgagccg
     4501 gttggaaaca aaacgggtgt tggggttgag gtcttcctgt gtcggcagca tcttctcgcg
     4561 gcccaggtcg atctggttcc tggccacgcg ggctatttca ccgaaaacga gaagcaggta
     4621 aatgtaaagt tcgtaggtgc ggtcaatgct gcgcatcagc tcacgttcgc cccgcgccat
     4681 atctccgtct tccgactgaa aatatccgta caacgcctgg agcgctttaa tcctcaggta
     4741 tctcctgttc agcatagtgt tttaaagaac gatgaaacgt ttcgttttca gcgtttggtc
     4801 ctgatcttcg cgacagcctc gatgcgggct tccgccatgc ggttggccgc catgaaggtg
     4861 ggtatgttct cttttttgga ggctccgaaa atggaggacg tagtattgaa aatattttcc
     4921 gcctggctca tcgcgtattc tcttccgtaa ccggccagct cggaatatac attgataagg
     4981 cctcctgcat tcacgacata atccggcgca tacaggattc ctttttccat cacgatcttt
     5041 ccgtggacgt tctcgtcggc gagctggttg ttggccgcac cgcagacgat agagcatttc
     5101 aggcgcgcaa gattttcgtc gttcagcgta gcacccagcg cgcagggagc gtagacgtcg
     5161 atatcgaggt cgaaaaacgc atcacccgca acgaattcca ccccgctgtt gtttttcttc
     5221 acgtccgcaa cacggtcttc ataaatatct gtgatgaaaa ctttagcgcc ttccctgacc
     5281 aggtgtgcga cgaggttcga gccaacgtgg ccgacgccct gcacggcaat ttttttcccg
     5341 ctgaggctgt cattccccca ccgctctttc gcagccgcct tcatccccat ataaacgccg
     5401 taggctgtaa cgggtgaagg atcgccgctt ccgcccagcg ccggcgggat gccggtgacg
     5461 tgcttcgttt ccatgaacac atattccatg tcgcgggaat tgatgccaac gtcttccgcc
     5521 gtaatgtatt ttccgccgag gctgttcacg aatttgccga aacggcgcat gagcatctcg
     5581 ttcttgtctt ttcttgaatc tgcaatgatg acggcttttc cgcccccgag attaagcccg
     5641 gtgatcgcag ctttgtaagt catcccgcgc gacaggcgca gcacgtcggt gagcgcttcg
     5701 gcttccgtgg cataaggcca catgcgtgtt ccgcccagcg cgggcccgag gaccgtattg
     5761 tgaactgcga tgatggaacg cagtcccgtt gcattgtcat tgcagaaaac cacctgctca
     5821 tgatcgaacg tgctcatctg cgaaagaggc cctgcggcaa cggaggacgt tttcgggtcg
     5881 ttggctgtaa gcatatcctt tgtaaggttt cgtcccgtcg tttcgggaat gaattactta
     5941 actttgcggc gtcagtttca ccggcggtca caaaagtagc tgtttttttc gcccacgtga
     6001 tgcgcaaaat attttaataa ttgaaggcgc tcggttatct caataaatat ttctggaaat
     6061 accgcctgcg gtttttttcc gggattttat tcatcattat ttccactttt tttcttaccc
     6121 gcccggttga attgctcggc aagggtatcg attttgcgat caatgcgaac aggttccatt
     6181 atccttccgg accgacggcg cacgtgctcc tgctctacgg tctcgagatc atttccgata
     6241 cgctcattta cggtttcttc cttttcctga accggcagac gatcatcgtc atgtcgcgcc
     6301 tcatcg
//
LOCUS       DASTSF010000516         8300 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1391406,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000516 DASTSF010000000
VERSION     DASTSF010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 8300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8300
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1391406"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(183..2762)
                     /locus_tag="VFU15_14720"
     CDS             complement(183..2762)
                     /locus_tag="VFU15_14720"
                     /inference="COORDINATES: protein motif:HMM:NF039501.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5686 and carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEU4719094.1"
                     /translation="MRAFLLILFSALVVSLHAGVVKGTVRDTTGQPVPFVAVVVKNST
                     YGVNTNLNGGYFLELKPGTYTLVFSQLGYEPQEHVVVISDEKTIVLDVTMHVSSKMLD
                     IFEVTAKGDRDKGKEIMRHVIDARADYWNKVQNYKCSTYEKSSLEKDLIDKPDDSVKV
                     VKPAPAAKDTTSKKKGGKGKDDLDNAIAHKQLNLIEAVSTTYFRAPNSFKEDILAYHD
                     YAEKKHYDGTGYSKNVDYGEHEIAPVQYYSENPYLLISDAQSADFNFYRNEIDAPALC
                     SRPLLSPAAGTAFLSYRFDYFTSFVENGKTIHKIGVTPVFGSDALFSGFIFIEDSTWA
                     IVSVNLSINPTVLLFSKDFNVIEDYKEVAPGIYLPVRCEFSYTIREGKYNIIGNTRID
                     RSDYVVNTTLPDKIFGDEVKHYADDAFDKDSAYWSDHRTIQLDEKEISYIHNVDSLVA
                     YYESPEYLSSQDSAFNHLTVWSFLLNGVGHRNRAKGNEFYFDPLIAQVVPFGVGGYRH
                     RLGGYYNHTFPNAMLMETEEQIDYGFLNHDVRGKVGVGLTYVPLHFVRTFIRVGDYYD
                     MINNYASLESVFARSNYVRCRQFSVAQRMEIVNGLFGELTFEYSDQFPITGMKLEQWS
                     QQVFGSLNTPADFDRYVKSEIRLELKYRFRQKYIIKDHRKIILGSKFPEIRFLYRKGL
                     PGVFGSEVDFDYIEFSSIDELKIGRFGTSDWALLAGAFVNKKDLRLLEYKYFRGSDSF
                     LFSDPLRSFQLLGPTLNTADAYFRLNYIHHFEGAFGSKIPLYGKLRITTAFGGGLLFI
                     PSADFYHEEFFVGLERIFRIRKQLFRFGVFAVTADSNVTKAAITYKVGVSFYNTFTRK
                     WSY"
     gene            complement(2868..3452)
                     /locus_tag="VFU15_14725"
     CDS             complement(2868..3452)
                     /locus_tag="VFU15_14725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719095.1"
                     /translation="MYRTGLLLFLWLIFFGGTATHTYSSFVADDEIVGFIHQQIAANH
                     ADRSEVRMRLWDVHIIPWDSADVKSVGLADSMSDDFFLYYEPRYTLDSVLQDSDKIFM
                     RKQVWAYSGKKQDWQKEDLRGVKLIQPVPPDQVHIGNEYWEFSLPVFSKDHNWCIAKY
                     EFVCGSRCIDRRTFLYKKSAEGNWLRYKLLRKDS"
     gene            complement(3484..4503)
                     /locus_tag="VFU15_14730"
     CDS             complement(3484..4503)
                     /locus_tag="VFU15_14730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014796467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU4719096.1"
                     /translation="MTAHFSIDIHTHILPENIPHWKEKFGYGGFIHLEHHKPCCAKMM
                     RDDGKFFREVEDNCWSAEKRMQECDHHHVNVQVLSTVPVMFSYWAKPQDCLELSKFLN
                     DHIAEIVHRFPKRFIGLGTIPMQDPKLAIQELERCKAIGMKGVQIGTNVNQENLSEPK
                     YHEIFAACEKLGMAVFVHPWEMMGEKDMQKYWLPWLVGMPAETSRAICSFIFGGVLEK
                     FPGLRIAFAHGGGSFPATVGRVQHGFDCRPDLVAIDNSVPPKNYLGKFWLDSLVHDAR
                     MLDVIVDMFGASRVALGSDYPFPLGENVPGELIKTMPWDDAKKNLLLHGAALEWLNMS
                     RKNFE"
     gene            complement(4622..5143)
                     /locus_tag="VFU15_14735"
     CDS             complement(4622..5143)
                     /locus_tag="VFU15_14735"
                     /EC_number="1.13.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014797852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000334 - 3-hydroxyanthranilate
                     3,4-dioxygenase activity [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyanthranilate 3,4-dioxygenase"
                     /protein_id="HEU4719097.1"
                     /translation="MITRPFNFKKWIDENRHLLKPPVNNKVVYRDTEFIVMVVGGPNS
                     RKDFHYNESEEFFYQLEGDIKVRIQEDGKAVEVPIKEGDIFLLPPKVPHNPMRGPDTV
                     GLVMERKRREGEKDGLMWFCEKCNNKLYDEYFTLTDITKQFQVVFEKFYGSEELRTCK
                     KCGAVMEPPPVIK"
     gene            5294..6637
                     /locus_tag="VFU15_14740"
     CDS             5294..6637
                     /locus_tag="VFU15_14740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719098.1"
                     /translation="MENKIQCPQCGAASSTKISDTEYHCVYCNSNFQVELSKAEMIAS
                     LLHKLEEPPKKSDFREQVEKLKAEALRSPEKMAEIRRRNRITVSIILGFFVVLGLVIF
                     FSARSGKSGTTIAGTDGSWETPSVYRFQVFAGSKGPVIWIISEQSGYSLDSARFTLKI
                     INPVNNTTLTEFPVVPDMSWHAGFDIDKTLGDFYACGDTCWVISENAGLTARDIYTGK
                     IIIDEKQMKLVDPKLSAGVSKASYRYYDHNFEVTTNDGFEYIFSPGMKHVFTKDEWKQ
                     LGRTRSVVKTFFILTETKRPQLFCTKEKTSPVESSSSVFTNELENFTPGKRPDNISDN
                     VLSIDHLLPDKTFFNGKIEYADSAETIVMYQDGIGQNAGVHVCSISSAGNQLWDTGGD
                     ALKNFRERFAETSGGIDFVYSPSGIILYSQYAGESAEAFDWKTGKLLWSHTAKKS"
     gene            6669..7451
                     /locus_tag="VFU15_14745"
     CDS             6669..7451
                     /locus_tag="VFU15_14745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007650617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU4719099.1"
                     /translation="MNLDLTGKHALVCGSTQGIGKAAATELAALGANVTLIARNEPRL
                     KNAVADLPGSGHHDYLVADFSDPGKLRETVAAYLRKEKIIHILVNNTGGPPSGPITEA
                     KPEEFMQAFSSHLICNHVLVQEVLPGMKQEKFGRIINVISTSVKIPLRNLGVSNTVRA
                     AVGNWAKTLANEVGAFGITVNNILPGATATQRLHSIIDNKVQKTGITKEEAEKEMIRE
                     IPAGRFGDPSEIAAAVAFLASPAAAYINGINVPVDGGRTGSL"
     gene            7526..7948
                     /gene="mscL"
                     /locus_tag="VFU15_14750"
     CDS             7526..7948
                     /gene="mscL"
                     /locus_tag="VFU15_14750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014595898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEU4719100.1"
                     /translation="MGFIKEFKEFAMKGNLVDMAVAFVMGGAFGKVVSSFIDGMIMPV
                     VGKLTTGVDFNAMKYVLSEATYDASGKIVKAEASIKYGAFITVIIDFILVAFVMFMVV
                     KAMNRMKKKQAEAPAPAPEPTKEELLLTEIRDLLKEKK"
     gene            7962..>8300
                     /locus_tag="VFU15_14755"
     CDS             7962..>8300
                     /locus_tag="VFU15_14755"
                     /inference="COORDINATES: protein motif:HMM:NF022720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3078 domain-containing protein"
                     /protein_id="HEU4719101.1"
                     /translation="MKKILLLVLLFPAMLFSRNLVPAKDSIPDSLRTWHYGGLASLNL
                     AQASYTDWAAGGVNSVSAQALFSGYLGYKKATTTWDNTLDLAYGILQQGKTDGVRKMD
                     DKIDFTSKLGH"
BASE COUNT         1996 a         2208 c         2182 g         1914 t
ORIGIN      
        1 ataatgacgg gaatcgcgaa aataaaaccg gtcttttccc cggctataaa aacaaccgcc
       61 gccgaaagaa gtatcgtggc cataacaatg acccagctgc gcagcatatc tttccacgga
      121 atcttcaagg gggttaataa acgaatggtg aatgtatggc ggaataatgg cggcgcaaac
      181 ggtcagtagc tccacttccg cgtgaacgtg ttgtagaagc taacgcccac tttgtacgtg
      241 atggccgctt tcgtcacgtt gctgtcggcg gtgacggcaa aaacaccgaa acggaaaagt
      301 tgcttgcgta tgcggaaaat gcgctcgagg ccgacgaaga attcctcgtg gtaaaaatcc
      361 gcgctgggaa tgaacagcag tccgccgccg aaggcggtgg tgatcctcag ctttccgtag
      421 agcgggattt tacttccgaa cgcgccttcg aaatgatgaa tgtagttgag ccggaaataa
      481 gcgtcggcgg tgttcagcgt cggacccagc agctggaaag accggagcgg atctgaaaaa
      541 aggaatgaat ccgaaccgcg gaaatacttg tactcgagca ggcggagatc tttcttgttc
      601 acgaatgcgc ctgccagcag ggcccagtcg ctcgtaccga aacggccgat cttcagctcg
      661 tcgatggagc tgaactcgat gtaatcgaaa tcgacctcgc tgccgaacac gccgggaagg
      721 cctttgcggt aaaggaaacg tatctccggg aatttcgacc cgagaattat tttgcggtga
      781 tctttgatga tgtatttctg gcggaaccgg tacttcagct cgagacggat ttccgatttt
      841 acataacggt cgaaatccgc cggcgtgttg agcgatccga aaacctgctg cgaccattgc
      901 tcgagcttca tgcccgtgat cgggaactgg tcgctgtatt cgaaggtcag ctcaccgaaa
      961 agcccgttca cgatctccat gcgctgcgca acgctgaact ggcggcagcg gacataatta
     1021 ctgcgcgcga aaacagattc gagcgaagcg taattgttga tcatgtcgta ataatcgccc
     1081 acgcggatga acgtgcgcac gaaatgcagc ggaacgtacg tcagccccac gcccactttc
     1141 ccgcgcacgt cgtggttgag aaaaccgtaa tcgatctgct cttcggtttc catcagcatg
     1201 gcgttcggga acgtgtggtt gtaatacccg ccgaggcgat gacggtagcc gcccacaccg
     1261 aacggaacga cctgcgcgat gagcggatcg aaatagaatt catttccctt cgcgcggttg
     1321 cggtggccca cgccgttgag caaaaagctc cacacggtaa gatggttgaa cgcggaatcc
     1381 tgcgatgaaa gatattccgg gctttcgtaa tacgccacga gactgtcgac gttgtggatg
     1441 taggaaattt ctttttcgtc gagctgtatc gtgcggtgat cgctccagta ggcgctgtcc
     1501 ttgtcgaacg catcgtcggc gtaatgcttc acttcatcac cgaagatctt atccggcagc
     1561 gtcgtgttca ctacgtaatc cgaacggtcg atgcgcgtgt tgccgatgat gttgtatttg
     1621 ccctcgcgga tggtgtatga aaattcacag cgcaccggca ggtagatacc cggggcgact
     1681 tctttgtaat cttcgatgac gttgaaatcc ttgctgaaaa ggagtaccgt gggattgatg
     1741 ctgaggttca ccgaaacgat cgcccacgtg ctgtcttcaa tgaaaataaa acccgagaac
     1801 agcgcatccg atccgaaaac aggcgtcacg ccgatcttgt gaatggtttt cccgttctcg
     1861 acgaaggacg tgaagtaatc gaaccggtag ctgagaaaag cggttcctgc tgcgggcgaa
     1921 agcagcgggc gcgagcacag cgcgggcgca tcgatctcgt tcctgtagaa attaaaatcc
     1981 gcgctctgcg catcgctgat cagcaggtac gggttttcgg aataatactg cacgggtgcg
     2041 atttcatgct cgccgtaatc gacgttcttc gagtaccccg ttccgtcgta atgttttttc
     2101 tcggcgtaat cgtggtaagc gaggatgtct tccttgaacg aattcggtgc gcggaaatac
     2161 gtggtggaaa ccgcttcgat aaggttgagc tgcttgtgcg cgatggcatt atcgagatca
     2221 tccttgcctt tgccgccttt ctttttcgag gtcgtgtctt tcgctgcggg ggcgggcttc
     2281 accactttca ccgaatcatc cggcttgtcg ataaggtctt tttccagcga tgatttttca
     2341 tacgtgctgc atttgtaatt ctgcactttg ttccagtaat ccgcgcgcgc gtcgatgacg
     2401 tgacgcatga tctcttttcc cttgtcacgg tcgcctttgg cggtcacctc gaaaatatcg
     2461 agcattttcg acgacacgtg catcgtcacg tcgagcacga tggttttttc atccgaaatg
     2521 acgacgacat gctcctgcgg ttcgtagccg agctgtgaaa agacgagcgt gtacgttccc
     2581 ggcttcaact cgaggaaata accgccgttg agattcgtgt tgacgccgta cgtggaattc
     2641 ttcaccacca ccgccacgaa cggaacaggc tgaccggtcg tatcgcgcac ggttcctttg
     2701 acaacgccgg cgtggagcga aacaacaaga gcagaaaaaa gaatgagcag aaaagcgcgc
     2761 atcgcgggtg aacaaaggta ggctttgtcg gctctgggac ggagattggc agggaattgt
     2821 tacaaccggt aaatgttaaa tgagggaatg taaatggtgt cgcgagctta cgaatccttc
     2881 ctcagcagct tgtaccgcag ccaattccct tccgctgatt ttttatacag gaatgttctc
     2941 ctgtcgatgc aacggctgcc gcagacaaat tcatatttgg caatgcacca gttatggtct
     3001 ttggagaaaa cgggcaggga aaattcccag tattcattcc ctatgtgcac ctggtccggc
     3061 ggcacaggct ggatgagttt cacgccgcgg aggtcttctt tctgccagtc ctgttttttc
     3121 ccgctgtatg cccaaacctg tttccgcata aaaattttat cgctgtcctg cagaacggaa
     3181 tcgagcgtat agcgtggctc gtaatagagg aaaaaatcat ctgacatcga atccgccagt
     3241 ccaacggatt tcacatcggc cgaatcccac ggaataatgt gcacgtccca caatctcatt
     3301 ctcacctcgc tcctgtccgc gtgattggcg gcgatctgct ggtgaatgaa accgacgatc
     3361 tcgtcgtcag cgacaaagga gctgtacgtg tgcgtagcag tcccgccgaa gaaaatgagc
     3421 cagaggaaaa gaaggagccc ggtgcggtac atgggttatg gtgaaaggat cagtgcgaac
     3481 atctcattcg aaattcttcc tgctcatgtt gagccattcc agcgccgcgc cgtgcagcag
     3541 caaattcttt ttcgcatcgt cccagggcat ggttttgatc agctcgccgg gaacattctc
     3601 accgagcggg aacgggtaat cgctgccgag cgccacgcgg ctggcgccga acatatcgac
     3661 gatgacatcg agcatgcgcg cgtcgtgcac gagcgaatcg agccagaatt tcccgaggta
     3721 atttttcggc ggaacgctgt tgtcgatcgc aacaagatcg gggcggcagt cgaaaccgtg
     3781 ctgcacacga ccgaccgttg cgggaaacga tccgcccccg tgcgcgaacg cgatgcgcag
     3841 ccccgggaat ttttccagca cgccgccgaa gatgaacgag cagatggcgc gcgacgtttc
     3901 cgcgggcatg cccacgagcc agggcagcca gtatttctgc atgtctttct cgcccatcat
     3961 ttcccaggga tgaacgaata cggccatgcc cagtttttcg cacgccgcga aaatctcatg
     4021 gtatttcggt tctgataaat tttcctggtt cacattcgtg ccgatctgca cgcctttcat
     4081 gccgatcgcc ttgcagcgtt ccagttcctg tatggccagc ttcgggtcct gcatcgggat
     4141 tgtgcccagc ccgatgaaac gtttcgggaa acgatggaca atttccgcaa tgtgatcatt
     4201 caggaatttc gagagctcga gacagtcctg cggtttcgcc cagtagctga acatcacggg
     4261 aaccgtggac agcacctgca cgttcacatg gtgatgatcg cattcttgca ttcttttttc
     4321 ggcgctccag caattgtctt ccacttcgcg gaaaaactta ccgtcgtcgc gcatcatctt
     4381 cgcacagcag ggtttgtggt gctcgaggtg aatgaacccg ccgtaaccga acttttcctt
     4441 ccagtgcgga atattttcag gcaggatgtg cgtatgaata tctatcgaga aatgagctgt
     4501 catctgggaa taggaaaatc cgggagatca aaagactggc aagtcaaaac catcaacaac
     4561 acttatctcc ttctttcgat ccgtccatct tttgaatttt tcaatcttat ttatcatcct
     4621 gttatttgat caccggcggc ggctccatca ccgccccgca ttttttgcac gtacgcagct
     4681 cttcgcttcc gtagaatttt tcaaacacca cctggaactg cttcgtgata tccgtcaatg
     4741 tgaaatattc gtcgtacaat ttgttgttgc atttttcaca gaaccacatc agcccgtctt
     4801 tctcgccttc gcgccgcttt ctttccatca ccagcccgac ggtatccggt ccgcgcatcg
     4861 ggttgtgcgg aactttcggc ggaagaagaa agatgtcgcc ttctttgatc gggacttcca
     4921 ccgctttgcc gtcttcctgt atcctgacct taatatcacc ctcgagctgg tagaaaaact
     4981 cttcgctttc gttgtaatga aaatctttcc gcgaattcgg tccgccgaca accatgacga
     5041 tgaattccgt atcccggtac acgactttgt tgtttacggg cggtttgaga agatgacggt
     5101 tctcgtcgat ccactttttg aaattgaacg gacgtgtgat catgatgaaa agattagttg
     5161 tgagctaacg aagttaggaa ttatggacgc acgcaggaat gacaattgac aggacggaaa
     5221 tttattccct gcgatggctg ccgggataag ccgttcttta tatttgttca ccactttttc
     5281 tccttacact tttatggaaa acaagatcca gtgcccgcaa tgcggcgcag ccagttcgac
     5341 gaaaatttcc gacacggaat atcactgcgt ttattgcaac agcaatttcc aggtggagct
     5401 ttcgaaagct gagatgatcg ccagcctgct gcacaagctg gaagagcctc cgaagaaatc
     5461 cgatttccgt gagcaggtgg aaaagctgaa ggcagaggcc ctgcgttccc cggaaaagat
     5521 ggcggagatc cgccgcagga accgcatcac ggtttcgatc atcctcggtt ttttcgtggt
     5581 tctcgggctg gtcatctttt tcagcgcacg ctccgggaaa tccggaacaa cgattgcagg
     5641 aacggacggc agctgggaaa cgccttccgt ttaccgcttc caggttttcg cgggaagcaa
     5701 aggtcccgtg atctggatca tcagcgaaca atcgggctac agcctcgaca gcgccaggtt
     5761 cacgttgaag atcatcaacc cggtgaacaa taccacgctc accgaatttc ccgtagttcc
     5821 cgacatgtcg tggcacgccg ggttcgatat cgacaaaacg ctcggcgatt tttatgcctg
     5881 cggcgataca tgctgggtca tctcggaaaa tgccgggctc accgcgcgcg acatttacac
     5941 gggaaaaatc atcatcgacg aaaagcagat gaagctggtg gatcccaaac tttccgccgg
     6001 cgtgagcaaa gccagctacc ggtactacga tcacaacttc gaggttacga cgaacgacgg
     6061 gttcgaatat attttcagcc cgggcatgaa acacgtgttc acgaaagacg aatggaaaca
     6121 gctcgggcgc acacggtctg ttgtgaaaac ttttttcatc cttaccgaga ccaaacgtcc
     6181 acagctgttc tgcacaaagg aaaaaacatc cccggtcgaa agctcttctt ctgtttttac
     6241 gaacgagctg gaaaatttta cgccgggaaa acgacctgac aacatcagcg acaacgtgct
     6301 ttccatcgat catcttcttc ccgacaaaac gtttttcaac ggcaagatcg aatacgccga
     6361 cagcgccgaa acgatcgtga tgtaccagga cggcatcggg caaaatgccg gcgtgcatgt
     6421 gtgcagcatt tcttcggccg gaaaccagct ctgggacacg ggcggcgacg cgctcaaaaa
     6481 tttcagggaa cgcttcgcgg agaccagcgg cggcattgat ttcgtttatt caccgtcggg
     6541 catcattctc tattcgcagt acgcaggtga aagcgccgaa gcgttcgact ggaaaacggg
     6601 caaattactg tggagccaca cagctaaaaa aagctgacac cggaagcttc gctcattatc
     6661 tttgcgccat gaatctcgac ctaaccggaa aacatgcgct cgtctgcgga agcacgcagg
     6721 gcatcggcaa agcggcggcc actgagctcg ctgcgctggg cgcgaacgtc acgctgatcg
     6781 cgcgcaacga accgcggctg aaaaacgcgg ttgcggatct tcccggcagc gggcaccacg
     6841 attacctcgt cgctgatttt tcagatccgg gaaagctgcg cgaaacagtt gccgcctacc
     6901 tgcggaaaga aaagatcatc cacattctcg tcaacaatac cggcggacct ccttccggtc
     6961 ccatcaccga agcgaagccg gaagaattca tgcaggcgtt ttccagtcac cttatctgca
     7021 atcacgttct cgtgcaggaa gtattgcccg ggatgaagca ggaaaaattc ggccgcatca
     7081 tcaatgtcat ttccacgtcg gtgaaaatcc cgctgaggaa tctcggcgtg tcgaatacgg
     7141 tgcgcgcggc tgtggggaac tgggccaaaa cgctggcgaa tgaagtgggc gcgttcggca
     7201 tcacggtgaa caatattctt cccggcgcaa ccgccacgca gcgcctgcac agcatcatcg
     7261 acaacaaagt gcagaaaacg ggcatcacga aagaagaagc ggaaaaagaa atgatacgcg
     7321 aaatccctgc cgggcgtttc ggcgatccgt cggagatcgc tgcggccgtc gcgttcctgg
     7381 caagtcctgc agctgcgtat atcaacggca tcaatgttcc cgtcgacggg ggaagaacgg
     7441 gatcactgta attctttaac agcaacgcta caatcacctt tcaataagtt tactcttcct
     7501 cactaacatt acaaacttca catccatggg tttcatcaaa gaattcaaag agttcgccat
     7561 gaagggcaac ctggtcgaca tggccgttgc atttgtcatg ggcggcgcat tcggaaaagt
     7621 ggtttcctcc ttcatcgacg gaatgatcat gccggttgtc ggaaagctga ccaccggggt
     7681 cgatttcaac gccatgaaat atgtgctcag cgaagcgaca tacgatgcgt cggggaaaat
     7741 cgtgaaggcg gaagcgtcga tcaagtacgg cgcattcatt acggtgatca tcgacttcat
     7801 ccttgtcgca ttcgtcatgt tcatggtggt aaaggcgatg aaccgaatga agaaaaagca
     7861 ggcggaagct cccgcgcctg cgcccgaacc cacgaaagaa gaattgctgc tcacggagat
     7921 ccgcgatctc ctgaaagaaa aaaaataacc acactaccgt catgaaaaaa atcctgctcc
     7981 tcgttcttct ttttcccgcg atgctttttt cgcgcaacct ggttccggcg aaagactcca
     8041 ttcccgacag tctccgcacc tggcattacg gcgggctcgc ttcgctgaat cttgcgcagg
     8101 cgagctatac cgactgggcg gcgggcggcg tgaattccgt ttcggcacag gcactgttca
     8161 gcggctatct cggttataaa aaggcaacga ccacatggga caacacgctc gatcttgcgt
     8221 acggcatcct gcagcaggga aaaacggacg gcgtgcggaa aatggacgac aagatcgatt
     8281 tcacctcgaa gctcggccat
//
LOCUS       DASTSF010000517        18049 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_319351,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000517 DASTSF010000000
VERSION     DASTSF010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 18049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18049
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_319351"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1609
                     /locus_tag="VFU15_14760"
     CDS             <1..1609
                     /locus_tag="VFU15_14760"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEU4719102.1"
                     /translation="DVPRNGNKNKPAADSVNGTKMPVPAAVNMMAVAFTGGTLALSDA
                     VNNINAVKGGKYELVIYQSTALGNGNQSQNPWLQEMPDPISKITWDNYVSMHPADVKA
                     KNLFGVGDDNLTVRFDELEDELDMVTVTANGVSVKLPVWAQPGIAKGTIAIAIGYGRN
                     GFNEVIDSVGANIYPAVGMNASGTMNYDVYDVSISDATGDKYPIATTQQHSTIMGRNE
                     DLLHETVLSAYLKDEHAGNPKPMMHTYKGEVNTEDVNLWNSFDRPNHKWALAIDLNSC
                     IGCGACLVSCNAENNVPVVGKDQVRKSREMHWIRIDRYYSSDTVKEEAETKGDTSTRE
                     MYRMMESPTFDNPKVVFQPLMCQHCNHAPCETVCPVLATNHSSEGLNMMTYNRCVGTR
                     YCANNCPYKVRRFNWFNYVEYHPFKDVNPAQDELSRMVLNPDVVVRSRGVMEKCSMCV
                     QRIQAGKLDAKKAGRRPIDGEINTACAQSCPTNALVFGDLNDENSEVSKWYNSGRKFE
                     MLEELGVRPSVFYLTKVWNREDEKAHV"
     gene            1644..3059
                     /gene="nrfD"
                     /locus_tag="VFU15_14765"
     CDS             1644..3059
                     /gene="nrfD"
                     /locus_tag="VFU15_14765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HEU4719103.1"
                     /translation="MQHESPLRDPLILGDKSYHDITTEISRPIEGKANMYWKLVFGIA
                     VTAFLWGFSCLAYTIGTGIGQWGANKTVGWAWDITNFVWWIGIGHAGTLISAVLLLFR
                     QKWRMAINRSAEAMTIFAVLCAAVFVTMHTGRPWVDYWLFPHANQFGSLWVNFNSPLM
                     WDVFAVSTYFSVSLVFWYLGLIPDFGAIRDRFKVLGKPMQEKIYRMLSFGWSGRARDW
                     SRFEEVSLVLAGLSTPLVFSVHSIVSMDFATSVVPGWHTTIFPPYFVAGAIFSGFAMV
                     STLLLIMRKVLNLEHYITMQHMEMMNIIITLTGSMVGVAYLSELFISWYSGVEYEGYA
                     FLNRATGPYWWSYASMMTCNVVVPQLFWVKKIRTSLIATFVLSIIVNIGMWFERFVII
                     VTSIHRDFVPSSWTMYHPTWVEVGIFIGTIGMFFTFFLLFARTFPVIALSELKSILKS
                     SGESYKKKRAEEKAKMAEAHH"
     gene            3082..3603
                     /locus_tag="VFU15_14770"
     CDS             3082..3603
                     /locus_tag="VFU15_14770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019975206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HEU4719104.1"
                     /translation="MVTTKTIHGIYDDEELLVEGAKKLRSERVRIKDVFSPFPIHGID
                     SVLGIPRTRIAICSFIYGITGTSLAILMTWYMMTGDWPTDIGGKPSFWYYMQWPAFIP
                     VTFELTVFCAAHGMAITFLLRSWLLPGVSPKNPDPRTTDNKFLVLVETTSEDESRIVS
                     MFRETGASEVNVI"
     gene            3628..4326
                     /locus_tag="VFU15_14775"
     CDS             3628..4326
                     /locus_tag="VFU15_14775"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEU4719105.1"
                     /translation="MKNKNIKLLAFGTFALMGAMLLNSCVKENPDSPGFEYMPDMYRS
                     PSVETNGKNAWLKDSVQMGNMIPPDGTVPRGFIPFPYPNTIQGDSLASLFWKNPLPKC
                     DSIEEEGKKLFEINCIYCHGPKGDGQGPLVTSNKYGATPPNYATKYADGVLTDGHIYH
                     VITYGKGNMGSHAYQLTPEERWKVIEYVQRLGRGGKPYSEWMKDQQKLTAAADSVAAK
                     NPKNGGATKTPPGK"
     gene            4354..5607
                     /locus_tag="VFU15_14780"
     CDS             4354..5607
                     /locus_tag="VFU15_14780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol:cytochrome C oxidoreductase"
                     /protein_id="HEU4719106.1"
                     /translation="MSHININNLTYNFSQKAQKNLFYLCGAGLLLLILGLIFHHAPEA
                     VGGQVIDEEKASLGQRVWANLLVNSYFFGGIALGATFFYALQYAAQASWSTVFMRVFS
                     AITTFLPIGLGLLFFILICGSMGLHHLYHWQDKEAYATDPIMQHKAPYFGAIFFYGRI
                     LVFFGGWIWYQRWARKNSLAEDLLKEGTTGNNWAVNLRNAAIFLVFFGFTSSVFSWDV
                     VMSVDTHWYSTLFGWYNFAGWWIGAIIVSNLIVMHLKSRDQMEYVNPSHVHDLGKWMF
                     AISFLWTYLWFSQFMLIWYSNIPEEVVYYQQRWENFRGLFWITMLVNFAFPMLVLMSR
                     DAKRNKSYLLFVALLIVFFHWTDTFLMIMPGTVGSNWGIGGIEIGMFLMFGSFFTWWI
                     LRSLSKAPILAKNHAFLEEGLHHHA"
     gene            5817..6722
                     /locus_tag="VFU15_14785"
     CDS             5817..6722
                     /locus_tag="VFU15_14785"
                     /inference="COORDINATES: protein motif:HMM:NF014809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HEU4719107.1"
                     /translation="MFFSFWQLFEYKDRLLPVAGSEHGADYDWLMNFNMVVIGIVFII
                     TNGLLFFMAFKYYGRDGGKAEYYPHNNKLEMLWTGVPAVVLFVIIFLGIRLWNNIMAP
                     APKGSRVIELYAQQFKWTVRYSGTDNELGKANYRLIKEGSNDLGLDSTDAAAYDDVIV
                     TDTLFLPVNEEADFRMRSRDVLHSAFFPYFRAQMNCVPGMQTYFHFKPIKTTDEMRKE
                     PSVINMMKGVNDVRRKAAEKEGKKFEPVQFDYLLLCNKICGAGHYNMQLLIYVGTKDQ
                     YNDFMKRHAPFYDGKTPPSKVAQIP"
     gene            6782..8611
                     /locus_tag="VFU15_14790"
     CDS             6782..8611
                     /locus_tag="VFU15_14790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEU4719108.1"
                     /translation="MHAHSELHAQTLEHDAHTDHGHDDHGHHETFISKYVFSMDHKVI
                     SRQFLVTAVIMACIAMVMSIVFRLQLAWPGEKFAFINAVMGDKWGPGGVLKPDAYLAL
                     VTIHGTIMVFMVLTGGLSGTFSNLLIPYQVGARDMASGFLNMLSYWFFFLSSLIMTIS
                     LFVEGGAASAGWTVYPPLSALPEAIPGSGLGMTLWLVSMSLFIVSSLLGGLNYIITVL
                     NFRTKGMKMTRLPLTVWAFFVTAILGVLSFPVLFSCALLLIFDRSFGTSFYLSDIYIN
                     GHALDQTGGSPILFEHLFWFLGHPEVYIIILPALGLTSEIISVNARKPIFGYRAMITS
                     ILAIGFLSFIVWGHHMFITGMNPFLGSVFVFTTLLIAIPSAVKAFNYITTLWKGNIQF
                     TPGMLFACGLVSTFITGGVTGIILADSALDINVHDTYFVVAHFHIVMGVSAILGMLAG
                     VYHWFPKLFLRHMNKNLGYMHFWLTFISAYGVFFPMHFLGLAGVPRRYYQNTAFPMFD
                     NLQDINVLITTFAIIGALAQLIFLFNFFYSMFRGEKSVQNPYGANTLEWTTPMAHTHG
                     NWPGELPEVHRWPYDYSKPGTDREFIPQTVPLGPGEEDGGSHH"
     gene            8675..9340
                     /locus_tag="VFU15_14795"
     CDS             8675..9340
                     /locus_tag="VFU15_14795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719109.1"
                     /translation="MNRRLLIWVALFLACCVNAHAQWDDTLRQIFHGSVYPSASFDTR
                     NSFVSSRRAHVWGLKIGVAFSDRIELGIGHNFLDDHIYKNVYYTSADGSMRMTEADLR
                     LWYSGVYAKYVYYTNRNWRFSIMPVQIGFGSSRYIYSDGRSELFRDKRMIAVYEPGIS
                     VSYKIVRWVGVGADLGWRYMLRDNPYIIENFNSPTYAFYVIVYWGEIYKDAFPNTRLA
                     KML"
     gene            9436..10470
                     /gene="ruvB"
                     /locus_tag="VFU15_14800"
     CDS             9436..10470
                     /gene="ruvB"
                     /locus_tag="VFU15_14800"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017843154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEU4719110.1"
                     /translation="MNPNIDPDSENLTPAEKEFEKLLRPQEFSDFAGQEGVVENLRIF
                     VQAAKQRGEALDHVLLHGPPGLGKTTLANIISQELGVNIKITSGPVLDKPGDLAGLLT
                     NLEKFDVLFIDEIHRLSPVVEEYLYSAMEDYRIDIMLDSGPSARSVQITLNPFTLVGA
                     TTRSGLLTSPLRARFGINARLGYYDAKLLKRIVERSAGILNVPVSDEASFEIARRSRG
                     TPRIANALLRRVRDFAQIKGNGSIDLEIAHYALQALNVDKNGLDEMDIRILSAIIDKF
                     KGGPVGITTISTAVGEEAGTIEEVYEPFLIQEGYLIRTPRGRQATEQAYKHLGRLPAK
                     DKGSLFDSDL"
     gene            complement(10518..11324)
                     /locus_tag="VFU15_14805"
     CDS             complement(10518..11324)
                     /locus_tag="VFU15_14805"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEU4719111.1"
                     /translation="MNRILVCTDFSPGSTNAMLYAAKLAAATSTTLVLVHVLHETGGK
                     PAGREKETAVNAKLLLTAEELEKRFKVNVNTEKRTGLPVDEIISAARKHRASLIVTGA
                     KETAAGSGLVGGLVYDLMHASLFPVLAVPVGCAFASYREVVFAVDPKNRDKYDDQLLR
                     ELTETFHSRLTIAVVTEPGTREKMMKDAAALVLEYNYAGLMHRLHVGESTNLEKGMQA
                     IVKQNEADLLVIIPQRNHYIDRLMQVTKTQKVLRNIDIPLLTLPRLTSAV"
     gene            complement(11416..12513)
                     /locus_tag="VFU15_14810"
     CDS             complement(11416..12513)
                     /locus_tag="VFU15_14810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719112.1"
                     /translation="MKKLLWIACIVPLLFSCRKDNSPETRCFYMGMTPWPPDFNAAGK
                     QLSYDFIRDNCDMISHHFDDGVPWEEFLNGDTLPKVLTDDVDYRLSKTPAGKKILLSV
                     APLDISRKAKAHYRSDIHNDPRENEWQAKRFSDTTVITAYSNYLSWLIAKFHPDFVNY
                     GVESNNGKWGDADFSDYKIFLDSVYHRMKRTYPALPFFLSFMVVDQDGFLSRARELEP
                     FTDFITISAYPYSYVGSPVEGSSSPSLIPPGLFPSYADINPSKPFAVAETGYIAEDLV
                     LPGVNKTGTPQWQEDYVNYIFKFCTDRHAQFLIWFCPIDYDAADNTFAAIGADQPLNH
                     LWQDTGLEDEWLTPRPAYQVWKEWVKKNVVQ"
     gene            complement(12517..12981)
                     /locus_tag="VFU15_14815"
     CDS             complement(12517..12981)
                     /locus_tag="VFU15_14815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719113.1"
                     /translation="MKRTAIVLLLLFTAGILPAQKDSTGLRKALTLYPLQKQIGYRSN
                     FTRKWFFDFKGGVTYSTLPFFNLEFNAARRMVITGKTAVYCGIGLTLDSYVPGVQVPF
                     GIETHPFSDIAQLGFIAEVSPKMTFGPTNFLNVAMPGHFGVAWYFRNASSKN"
     gene            13062..13991
                     /gene="queG"
                     /locus_tag="VFU15_14820"
     CDS             13062..13991
                     /gene="queG"
                     /locus_tag="VFU15_14820"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013070097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HEU4719114.1"
                     /translation="MDRKKYAAIVKEEARRAGFDYCGISKAEFLEEEAPRLEKWLREG
                     MQGEMSWMENHFELRLDPSKLVPGAKSVISLLLNYYTEKKQEDPAAPKISMYALGEDY
                     HFVIRRKLKELLNALQEKIGKEISGRVFVDSAPVMDKVWAKKSGLGWVGKNSNLITRE
                     MGSFFFIAELIVDIELEPDSPTGDFCGTCTKCIDACPTDAIVSPYVVDGSKCISYFTI
                     ELKNAIPESEKGKFENNMFGCDICQDVCPWNRFSRPHTTPEFGPDEQLLSMKKREWEE
                     LTEDVFDEIFRRSAVKRTKFAGLKRNIRFLKSS"
     gene            complement(13978..14172)
                     /locus_tag="VFU15_14825"
     CDS             complement(13978..14172)
                     /locus_tag="VFU15_14825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719115.1"
                     /translation="MLEFSQHILKSVSFDKSLFRKELSKMLKWVKPDEALLLKAWCIA
                     TFGHMYGDVIRDVYRSVTKT"
     gene            complement(14351..14920)
                     /locus_tag="VFU15_14830"
     CDS             complement(14351..14920)
                     /locus_tag="VFU15_14830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017258062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HEU4719116.1"
                     /translation="MKKQKIVIAVTGASGSIYAKVLLDRLVSLKEQVEEISLLISDNA
                     KEVWKTELGNTSYEKYPFRTFGKNDFSAPFASGSSSYRTMIVCPCSMGTLARIATGIS
                     NDLVTRAADVMLKERGKLILVPRETPLSLIHINNLKTVTEAGAIVYPASPSFYSKPAS
                     FEELAATVTDRILDQAGLEVKTFRWNENA"
     gene            complement(14924..15568)
                     /locus_tag="VFU15_14835"
     CDS             complement(14924..15568)
                     /locus_tag="VFU15_14835"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HEU4719117.1"
                     /translation="MRKKGFAFFLFASLLFLSRAHSQQTFRAGIQAGINGSQVHGDSY
                     SGFNKAGVVGGIFVCTDPTKRFYAQMEMQYSSKGSRKNPNPDKGDYTSFRLAMNYAEV
                     PFLLRMNSRNVYFEIGQTFGFLVNVREWDSNGEIHPAGFRKWETAFIFGAGINLNPHF
                     SVDLRYTNSVAPVLKFQVPLYYPNFFMNLFNKGMYHNVLGLTLAYRFGDSGTSQ"
     gene            complement(15631..16500)
                     /locus_tag="VFU15_14840"
     CDS             complement(15631..16500)
                     /locus_tag="VFU15_14840"
                     /inference="COORDINATES: protein motif:HMM:NF022985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Omp28-related outer membrane protein"
                     /protein_id="HEU4719118.1"
                     /translation="MKKHISLLAIIAFASWGCDYIEVPPYVGGSSVTPVSDTVRRILI
                     EDVTGHTCVNCPSAAKVIDSLEHLYPGQIVPVAVHFGFFAQPTQPAGTWPAGAFSEDF
                     RVTAEDADYDAIFGCSSWSLPNGLVNRTGYPSNIPSPAPTWSSTGAAILSTPMSAYLK
                     ITPTYTASSHTLNVQVSGEMMRDTTGTYLLALYLVEDSLTGYQLDNSVSLGYDSNYVF
                     NHVFRGCINTPGSIIGDTVLSGTIPAHTGISYSSPSFTINAAYNAAHCRVVGFIYKSD
                     DYGVLQAAEADVE"
     gene            complement(16526..>18049)
                     /locus_tag="VFU15_14845"
     CDS             complement(16526..>18049)
                     /locus_tag="VFU15_14845"
                     /inference="COORDINATES: protein motif:HMM:NF039629.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6029 family protein"
                     /protein_id="HEU4719119.1"
                     /translation="TVPEKALMNGWGNINYINGKFSAGLRYESYLNVMQGYPSGYKGT
                     GIPYRYATYNNDGLEITVGSAYEQFGSGLVLRAYEDRGLGYDNALDGMRVRYNPYKGI
                     YLKGVWGKQRNFFTTGPGIVRGFDGEINLNELFDSCMANVKTQFIFGGSFVSKYQPDL
                     NPSLILPQNVGTWAARGNIITSRINLYGEYAYKINDPTIGNGYIYKPGQALFTSATYS
                     QKGFGLYGAYMYVDNFGYRSDRDATGNSLMINYLPALTKQHTYLLAAFYPYASQPNGE
                     VCWQGEADYKIRKGTKLGGKYGTTITLNYSVAHGIDTTQLNDRLGKDYGYSAKWSAVG
                     DSLFYQDANIEIYREFFHRTASLLKVTVMYANMIYNKNVIEGHVNYAVVHANIGVVDV
                     TWKVNKHFTFHTDAEHMSVGKTNGVVQDQGNWAMLLEEVSIGNHFFIAGMDEWNYGNP
                     VADQRLHYYNVQAGFLKGTNRIAVGYGKQRAGIFCVGGVCRFVPASNGLTLTITSSF"
BASE COUNT         4287 a         4503 c         4831 g         4428 t
ORIGIN      
        1 ggatgttccc cggaacggca ataagaataa gccggcagcg gattcagtca acggaacgaa
       61 aatgccggta ccggctgcag tgaacatgat ggcggttgcc ttcaccggcg gtacgcttgc
      121 tctttccgat gcagtgaaca atatcaacgc ggtcaaagga ggaaaatacg agctcgtgat
      181 ctaccagtct accgctctcg gtaatggtaa ccagtctcaa aacccgtggc tgcaggaaat
      241 gcccgacccg atctctaaga tcacgtggga caattatgta tccatgcacc cggccgatgt
      301 aaaagcgaaa aatctcttcg gtgtgggtga tgataacctg accgttcgtt tcgatgagct
      361 cgaagacgag ctggatatgg tgacggttac ggcaaatggc gttagcgtaa agcttcccgt
      421 gtgggcgcag cccggaattg cgaaaggaac gatcgctatc gcgatcggtt acggacgcaa
      481 cgggttcaac gaagtgatcg acagcgtcgg tgccaatatt tatcccgccg tcggtatgaa
      541 tgcttccggt acgatgaatt atgatgtgta cgatgtcagc atcagtgatg caaccggtga
      601 caagtacccg atcgctacca cgcagcagca ttccacgatc atgggacgga atgaagattt
      661 gctccatgaa acagtgcttt cagcgtactt aaaagatgag catgccggca atccgaagcc
      721 gatgatgcat acatacaagg gcgaggtgaa cacggaagac gtcaacctgt ggaacagttt
      781 tgaccgtccg aatcacaaat gggcgcttgc catcgacctt aactcatgca tcggctgtgg
      841 cgcttgtctg gtcagctgta acgcggaaaa caacgttccg gtggtcggaa aagaccaggt
      901 ccgcaagagc cgtgaaatgc actggattcg catcgatcgc tattatagtt cagatactgt
      961 gaaagaggaa gcggaaacca aaggcgatac cagcacgaga gaaatgtacc gcatgatgga
     1021 aagcccgacg tttgataatc cgaaggtcgt tttccagccg cttatgtgcc agcactgcaa
     1081 ccatgctccg tgcgaaacgg tttgcccggt gcttgcgacc aatcacagct ccgaaggact
     1141 gaacatgatg acgtacaacc gttgtgtggg aacgcgctat tgtgcgaaca actgcccgta
     1201 caaagtgcgt cgcttcaact ggttcaacta tgtggaatat catccgttca aggatgtcaa
     1261 tcccgcacag gacgagctca gccgcatggt gctgaacccg gatgttgtcg ttcgttcccg
     1321 tggtgtgatg gaaaaatgct ccatgtgcgt tcagcgcatc caggccggaa agctcgatgc
     1381 gaaaaaggcc ggccgtcgcc cgatcgacgg ggaaatcaat acggcatgcg cgcaaagctg
     1441 tcccacgaac gcacttgttt tcggcgacct caacgacgag aacagcgagg tttcaaaatg
     1501 gtacaacagc gggcgcaaat tcgaaatgct cgaagagctc ggtgttcgtc cttccgtatt
     1561 ctacctcacg aaagtgtgga accgcgaaga cgaaaaagca cacgtgtaat aaattgaaag
     1621 agaacagaca ataagataac aatatgcaac acgaatctcc gcttagggac ccgctgattc
     1681 tcggtgataa gtcgtatcac gatatcacga ccgagatcag tcgtcctatt gaaggcaaag
     1741 cgaacatgta ctggaagctt gtcttcggaa ttgcggttac cgcatttctc tggggcttta
     1801 gctgtcttgc ttatactatc ggaacgggta taggacagtg gggcgcaaac aaaacggtag
     1861 gctgggcctg ggacatcacc aacttcgtct ggtggatcgg tatcggtcac gccggaacgc
     1921 ttatctccgc cgtactgctg ctgttccgtc agaaatggag gatggcgatc aaccgctctg
     1981 cggaagccat gacgatcttc gccgtattat gcgcagccgt tttcgtaacg atgcacacag
     2041 gtcgtccgtg ggtggattac tggctgttcc cgcatgcgaa ccagttcggt tccctctggg
     2101 tgaacttcaa ctccccgctg atgtgggacg ttttcgcggt atccacgtat ttttctgttt
     2161 cgcttgtttt ctggtacctc ggacttattc ccgatttcgg tgccatccgc gatcgcttca
     2221 aagttctcgg aaaaccgatg caggaaaaga tctatcgcat gctgagcttc ggctggagcg
     2281 gacgtgcccg cgactggtca cgttttgaag aagtttctct cgttcttgcc ggactttcca
     2341 cgcctctcgt attctctgtt cactcgatcg tatccatgga ctttgccact tcggttgttc
     2401 cgggatggca taccacgatc ttcccgcctt atttcgtcgc aggtgctatc ttctccggat
     2461 ttgcgatggt ttcgacgctt ctcctgatca tgcggaaggt gttgaacctc gaacattaca
     2521 ttacgatgca gcacatggaa atgatgaaca ttatcattac cctgaccgga tcaatggtcg
     2581 gcgttgctta cctgtcggag ctgtttattt cctggtattc gggtgtcgaa tatgaaggat
     2641 acgctttcct taaccgcgct accggtcctt actggtggag ttatgcttca atgatgacat
     2701 gtaacgttgt cgtaccgcag cttttctggg tgaagaagat ccgtacgagc ctcatcgcca
     2761 cgttcgttct ttccattatt gtgaacattg gtatgtggtt cgagcgtttc gtaattatcg
     2821 taacgtccat ccacagggat tttgttccgt cgtcatggac catgtatcac ccgacgtggg
     2881 tggaagtggg cattttcatc ggaacgatcg gaatgttctt tacgttcttc ctgctgtttg
     2941 ccagaacatt ccccgtgatc gcattgagtg aattgaaatc gatcctcaaa tcttcgggcg
     3001 aatcatacaa aaagaagcgt gccgaggaga aagcgaaaat ggctgaggcg catcattaat
     3061 gataaaacga ctgactgaat tatggtaacg accaaaacca tacacggaat ctacgacgat
     3121 gaagagcttc tcgtcgaggg agcgaaaaag ctgcgttcgg agcgtgtgcg catcaaggac
     3181 gttttctctc cgtttcctat tcatggaatt gattccgttc tcgggattcc aaggacgcgg
     3241 atcgctatct gctcgttcat ctacgggatc acgggaacat cgcttgcgat cctgatgacc
     3301 tggtatatga tgaccggcga ctggccgacg gacatcggtg gaaagccgag cttctggtat
     3361 tatatgcagt ggcccgcttt catcccggta acgttcgagc tcacggtttt ctgcgccgcg
     3421 cacggaatgg cgatcacttt cctgctcagg agctggctgc ttcccggtgt ttcaccgaag
     3481 aaccccgatc cgcgcactac cgacaacaag ttcctggtcc tggttgaaac aacttcagaa
     3541 gatgaatcac gcatcgtgag catgttccgc gaaaccggcg catcggaagt aaatgtgatc
     3601 tgatcagaaa gcgatagtaa cgatacaatg aaaaacaaaa acataaaact ccttgctttc
     3661 ggaacattcg ctttgatggg cgcaatgttg ctgaattcct gcgtcaagga aaacccggac
     3721 agtcccggat tcgaatacat gccggacatg tacagaagtc cttccgttga aacgaacggc
     3781 aagaacgcct ggctcaagga ttcggtccag atgggaaaca tgatcccgcc ggacggaact
     3841 gttccccgcg gtttcattcc gttcccttat cccaatacga tacagggtga cagtcttgcc
     3901 tcgctcttct ggaaaaaccc gctgccgaaa tgcgactcga tcgaagaaga aggaaaaaaa
     3961 cttttcgaga tcaattgcat ctactgccac ggaccgaaag gcgacgggca gggaccgctt
     4021 gtgaccagca acaaatatgg cgcgacgccc ccgaactacg caacgaaata tgccgacggc
     4081 gtattgaccg acggtcacat ttatcacgtc attacttatg gcaagggtaa catgggttcg
     4141 catgcatacc agcttacgcc tgaagaacgc tggaaggtga tcgaatatgt tcagcgtctg
     4201 ggtcgcggag gcaagccata cagcgaatgg atgaaggatc agcagaagct gacagcagca
     4261 gctgattcgg ttgccgcaaa aaatccgaag aacggcggtg caacaaaaac tcctcccgga
     4321 aaataaacga taacgaaaga tatctctgct gatatgagtc acattaacat caataatctt
     4381 acgtacaact tctcacagaa ggcgcagaag aacctcttct acctctgtgg cgcgggttta
     4441 cttctcctca ttctcgggct gatctttcat cacgcaccgg aagctgtggg cggccaggta
     4501 atcgatgaag agaaggcatc gctgggtcag cgtgtctggg cgaacctgct tgtgaacagc
     4561 tatttcttcg gcggaattgc gctcggagct acattctttt atgccctgca gtatgcggcg
     4621 caggcttcgt ggagcacggt tttcatgcgt gtgttcagcg ccattaccac tttccttcct
     4681 atcggtctcg ggctgctgtt cttcatcctg atctgcggat ctatggggct tcaccacctg
     4741 tatcactggc aggataaaga agcgtatgct accgatccga tcatgcagca caaagcgccg
     4801 tatttcggag cgatcttctt ttatggcagg atactcgtgt ttttcggtgg atggatctgg
     4861 taccagcgat gggcccgtaa aaattcactg gcagaagacc tgctgaaaga aggcactacc
     4921 gggaacaact gggcggtcaa cctccgcaac gctgcgatat tcctcgtgtt cttcggcttc
     4981 acttcctctg ttttcagctg ggatgtggtc atgtctgttg acacgcattg gtacagcacg
     5041 ctgttcggat ggtacaactt tgccgggtgg tggatcggcg cgatcatcgt ttccaacctg
     5101 atcgtaatgc atctcaaatc ccgtgaccag atggaatatg tgaaccccag ccacgtgcac
     5161 gatctcggca aatggatgtt cgccatcagc ttcctgtgga cctacctctg gttttcccag
     5221 ttcatgctta tctggtattc gaacattccc gaagaggttg tttattacca gcagcgctgg
     5281 gaaaatttcc gcggactttt ctggatcacg atgctggtga actttgcgtt cccgatgctt
     5341 gtgcttatgt cgcgtgacgc aaaacgcaac aagtcatacc ttcttttcgt ggcgcttctc
     5401 attgtgttct tccactggac ggatacattc ctgatgatca tgccgggaac cgttggttcc
     5461 aactggggga tcggtggcat cgaaatcgga atgttcctga tgttcggatc tttcttcacc
     5521 tggtggatcc tccgctcgct ttcaaaagca ccgatccttg cgaagaacca cgcattcctg
     5581 gaagaaggac tgcatcacca tgcttaatta aaacgaattt gtatccaaca gaataaccga
     5641 ccaacactat gcaattattg atctatctaa tcctgattct tgctgctgcc gccgtatggc
     5701 agctggttcg cgttgtggaa ttgtcaggca agctgcgggg ggcagacacc cacaaggtta
     5761 cagagcgcga caatcgcatg aatggccggc tgatgcttac cttttatctg ttgctgatgt
     5821 tcttcagctt ctggcagctg ttcgaataca aggacaggct tcttcctgtt gccggatcag
     5881 agcatggtgc tgactatgac tggctgatga actttaacat ggttgtcatc ggcatcgtat
     5941 tcattattac gaacggactt ctcttcttta tggcgttcaa atattatgga cgcgatggag
     6001 ggaaagccga atattatccg cacaacaaca agctcgagat gctgtggacg ggtgttcctg
     6061 ctgtcgttct tttcgtgatc attttccttg gtatccgcct ctggaataat atcatggcgc
     6121 ctgcacccaa agggtcgcgc gtgatcgagc tgtatgcaca gcagttcaaa tggacggtgc
     6181 gttacagcgg tacggataac gaacttggaa aagcaaatta tcgcctgatc aaagaaggat
     6241 ccaatgatct tggacttgat tctacggacg ctgcagctta cgacgatgtg atcgttaccg
     6301 atactctttt tcttcccgtg aatgaagaag cggatttccg catgcgttca cgggatgtgc
     6361 tgcacagcgc attcttcccg tatttcaggg cgcagatgaa ctgtgttccc ggaatgcaga
     6421 cctatttcca tttcaagccc atcaaaacaa cggacgagat gcgaaaggaa cccagtgtga
     6481 tcaacatgat gaagggtgta aatgacgttc gcaggaaagc ggccgaaaaa gaagggaaaa
     6541 agtttgaacc ggtgcagttt gattacctgc tcctgtgcaa caagatatgc ggcgccggcc
     6601 actacaatat gcagcttctc atttacgtgg gcacaaagga tcaatacaac gatttcatga
     6661 aacggcacgc tccattctac gacgggaaaa cacctccttc aaaagtggcg cagattccgt
     6721 aacgaactat tttctgaaat aaacgcaggg acgaacaaaa aataaattac caatggcaga
     6781 aatgcacgca catagtgagc tacatgcaca aacgctggag catgacgcac acaccgatca
     6841 cgggcatgac gatcatggtc accatgaaac gttcatatcc aagtatgtct tcagcatgga
     6901 ccataaggtc atctcccgcc agtttcttgt tacggcggtg atcatggcct gcattgcgat
     6961 ggtcatgtct atcgtattcc gtcttcagct ggcctggccg ggtgaaaaat tcgcattcat
     7021 caatgcggtc atgggcgaca agtggggccc gggcggagtt ctcaagccgg acgcatacct
     7081 tgctctcgtt acgatccacg gtaccatcat ggtgttcatg gtgctcaccg gcggattgag
     7141 cggaacattt tccaacctgc tgattcctta ccaggtgggt gcacgcgata tggcttcagg
     7201 cttcctgaac atgctttcct attggttctt cttcctgtca agccttatca tgaccatctc
     7261 ccttttcgtg gagggtggtg ctgcatccgc aggatggacg gtttacccgc ctttatccgc
     7321 gctgcctgaa gcgatcccgg gctcgggtct tggaatgacg ctttggctgg tttcaatgtc
     7381 cctgttcatc gtttcatcgc tgctgggtgg cctgaactac attatcacgg ttctcaattt
     7441 ccggacgaaa ggaatgaaga tgacgcgcct cccgcttacg gtgtgggcat tcttcgttac
     7501 cgcaattctc ggtgtccttt cgttcccggt tcttttctcc tgcgcgctcc tgctcatctt
     7561 cgaccgcagc ttcggaacga gcttttacct gtcggacatt tacatcaacg gacatgcgct
     7621 ggatcagacc ggcggcagcc cgatcctttt cgagcacctg ttctggttcc tcggtcaccc
     7681 ggaagtgtat atcattattc ttcctgcact cgggctgacg tctgaaatta tttccgtgaa
     7741 tgcccgcaag ccgatcttcg gttaccgtgc gatgatcacg tcgatccttg ccatcgggtt
     7801 cctgtccttc atcgtatggg gacaccacat gttcattacc ggcatgaatc cattccttgg
     7861 ttcggtgttc gtgtttacca cgctgctgat cgctattcct tccgcggtga aggcgttcaa
     7921 ctacattacg acattgtgga aaggaaacat ccagttcacc ccgggcatgc tgttcgcgtg
     7981 cgggctcgtg tcgacattca tcaccggcgg cgtaacgggt atcattcttg cggattctgc
     8041 gctcgatatc aacgtgcatg acacgtattt cgttgtggcc cacttccaca tcgttatggg
     8101 cgtttccgca atcctcggga tgcttgcagg cgtgtatcac tggttcccga aactattcct
     8161 gcgtcacatg aacaagaacc tcggctatat gcatttctgg ctgacgttca tcagcgcgta
     8221 cggcgtgttt ttcccgatgc acttcctcgg ccttgcgggt gttccgcgac ggtattacca
     8281 gaacactgct ttcccgatgt tcgacaatct gcaggacatc aacgtgctga tcacgacatt
     8341 cgcgatcatc ggtgcgcttg cgcagctgat cttcctgttc aacttcttct acagcatgtt
     8401 ccgcggtgag aaatcagttc aaaacccgta cggtgcgaac acgctcgaat ggacaacgcc
     8461 tatggcgcat acgcatggca actggccggg cgaacttccc gaagttcacc gctggccata
     8521 tgattacagc aagcccggca cagaccggga attcataccg caaacggtgc cgctgggtcc
     8581 gggcgaagaa gatggcggaa gccatcatta aaagaaatga aagactctcc cctgcgagag
     8641 tctttttttt agttgctgat tcaatctgac gaagttgaac cgaagactcc tgatttgggt
     8701 tgcgttgttc cttgcctgct gcgtgaatgc gcatgcacaa tgggacgaca cgctgagaca
     8761 gatctttcac ggaagcgttt atccttcggc ttcattcgat accaggaatt catttgtaag
     8821 ttcgagacgt gcccatgtgt ggggcctgaa aataggcgtc gctttttcgg atcgcatcga
     8881 gctcggcatc gggcataatt tcctcgatga tcatatttat aaaaacgtct attacacttc
     8941 tgccgatggc agcatgcgga tgacggaggc tgacctgcgt ctgtggtatt ccggcgtgta
     9001 tgcaaaatat gtttattaca cgaacaggaa ctggaggttc agcatcatgc cggtgcagat
     9061 cggtttcgga agttcgcgtt acatctacag cgatgggaga agcgagcttt tccgcgataa
     9121 gcgcatgatc gctgtgtacg aacccggaat atcagtttcg tataagatag tgcgatgggt
     9181 gggcgtaggc gcagaccttg gatggagata tatgctgcgc gacaacccgt atatcattga
     9241 aaacttcaat tcaccgacat atgcgttcta cgtgatcgtg tactggggcg aaatttacaa
     9301 ggacgcattc ccgaatacaa ggctggcgaa aatgctgtag cagccagcgc agtgaaacgg
     9361 aaatacgggg aagcaacttt tcattcttcg cataacggat aagaacattc gcgcactaca
     9421 tttgtaataa tgtccctgaa cccgaacatt gatccagaca gcgaaaacct gacgccggct
     9481 gaaaaggaat tcgaaaagct gctgcgcccg caggagtttt ccgattttgc cgggcaggaa
     9541 ggcgtggtgg agaacctcag gatatttgtc caggccgcga agcagcgcgg cgaagcgctg
     9601 gaccacgtgc tgctgcatgg tccgccggga ttgggaaaaa ccacgctggc gaatatcatc
     9661 tcacaggaac tgggcgtcaa catcaagatc acttcagggc cggttctcga caaacccggt
     9721 gacctcgcgg gattgcttac caacctggaa aaattcgatg tgctgttcat cgacgagatc
     9781 catcgcctca gcccggtggt ggaggaatat ctttactcgg ccatggaaga ttaccgcatc
     9841 gacatcatgc tcgacagcgg gcccagcgca agaagcgtgc agatcacgct caacccgttt
     9901 acgcttgtcg gcgccactac acgttcggga ttgcttacgt cgccgctgcg cgcacgtttc
     9961 ggtattaatg cgcggctcgg ttattacgat gcgaaactgc tgaaaagaat tgtcgaacgc
    10021 tccgcgggaa ttctgaacgt tcccgtttcg gatgaagctt catttgaaat tgcacgacgc
    10081 agtcgcggaa cgccgcgtat tgccaacgcc ctgctccggc gggtgcgtga tttcgcgcag
    10141 atcaaaggaa acggatccat tgatcttgaa attgcgcatt acgccctgca ggcgctgaac
    10201 gttgacaaga acgggctcga cgaaatggat atccgcatcc tttcggcgat catcgataaa
    10261 ttcaaaggcg gacccgtcgg gatcaccacg atctcaaccg ccgtgggcga agaagccgga
    10321 acgatcgagg aagtgtacga accttttctt atccaggaag gttatcttat ccggacaccg
    10381 cgcggaagac aggcaaccga acaggcgtac aagcatctcg gcaggttgcc ggccaaagac
    10441 aaaggcagcc tgttcgattc ggatctttag cctcgtaaag ctattgcgga gagcaggaaa
    10501 acaaaacgga ttcttgctta aacagcgctt gtcaaccgcg ggagtgtgag aagaggaatg
    10561 tcgatattcc gcagcacttt ttgtgttttc gtgacctgca tgaggcggtc gatgtaatga
    10621 ttgcgctggg gaatgatcac gagaagatca gcttcgttct gctttacgat tgcctgcatg
    10681 cccttttcaa ggttcgtgct ttccccgacg tgcaggcggt gcatcagccc ggcgtaattg
    10741 tattcgagga caagggccgc ggcatccttc atcatttttt cccgcgttcc gggttcggtg
    10801 acaacggcga tggtaagacg ggaatgaaaa gtttccgtta gctcacgcaa taactgatca
    10861 tcatacttgt cgcggttttt cggatcaaca gcgaacacga cttcccggta agacgcgaac
    10921 gcgcagccga ccggaactgc gagcacggga aacaaagaag cgtgcatgag atcgtatacc
    10981 agtccgccaa ccagcccgct gcccgccgcc gtttcttttg ctcctgtgac gatgagcgat
    11041 gcgcgatgtt ttctcgctgc ggaaataatt tcatcgacgg ggagaccggt tcttttttcc
    11101 gtattcacat tcactttgaa gcgcttctcc agttcctccg cagtaagcag aagctttgcg
    11161 ttgaccgccg tttctttttc gcgcccggca ggcttcccgc ctgtttcgtg aagaacatgc
    11221 accagtacta acgtcgtact cgtcgcggcg gcaagcttcg cggcataaag catcgcgttg
    11281 gtgctcccgg gggaaaaatc cgtgcagacg agaatccggt tcatgatgcg gagaatggac
    11341 agctaaagta atgtattaga ccgtgaaccg gcaagtacga agcccggggc tcccggtttt
    11401 gcggcgttta tatttttatt gaacaacatt ttttttcacc cactccttcc acacctggta
    11461 cgccgggcgc ggcgtcagcc attcatcttc gagcccggtg tcctgccaga gatgattcag
    11521 cggctggtcg gcgccgatgg ctgcaaatgt attatcggcc gcatcgtaat cgatgggaca
    11581 aaaccagatg aggaactgcg cgtgacggtc ggtgcagaat ttgaaaatgt aattcacgta
    11641 atcctcctgc cattgaggtg ttcctgtttt gttcacgccg ggcagaacaa gatcttccgc
    11701 gatgtagccg gtttctgcaa cggcgaaagg ttttgacgga ttgatgtcgg cataagaagg
    11761 aaagagaccg ggcggaatga gcgaaggact ggaagatcct tccaccggcg aaccgacata
    11821 cgaatacggg taggcggaaa tggtgatgaa atccgtgaaa ggttcgagct cgcgtgcgcg
    11881 cgaaagaaat ccgtcctggt caacgaccat gaagctcagg aaaaaaggca gcgctggata
    11941 cgtacgtttc atgcggtggt acaccgagtc gaggaagatt ttgtaatcgg aaaaatccgc
    12001 atcgccccat ttcccgttgt tgctttccac gccgtagttg acgaaatccg ggtggaattt
    12061 cgcgatgagc caggaaagat aattcgaata ggcggtgatg acggtcgtat cgctgaaacg
    12121 tttggcctgc cattcgtttt cacgcggatc gttgtggata tcggaacggt aatgcgcttt
    12181 tgcttttcgc gaaatatcga gcggtgcaac gctgagcagg attttttttc ctgcaggcgt
    12241 tttggaaaga cggtagtcaa catcgtcggt cagcactttt ggaagcgtat cgccattgag
    12301 aaattcctcc cacggaacgc cgtcgtcgaa gtgatgcgag atcatgtcgc agttgtcgcg
    12361 gatgaaatca taggaaagct gttttcctgc ggcgttgaaa tccggcggcc acggcgtcat
    12421 gcccatgtag aagcagcgcg tttccgggga attatccttc cggcaggaaa agagcagcgg
    12481 aacgatgcaa gctatccaga gcagcttttt catgcgtcag ttttttgaag atgcgtttcg
    12541 gaaataccac gccacgccaa aatgcccggg catggccacg ttgagaaaat ttgtcgggcc
    12601 gaatgtcatt ttcggggaaa cttcggcaat gaatcccagc tgtgcgatat cgctgaacgg
    12661 gtgcgtttca ataccgaacg ggacctgtac gcccggaaca tagctgtcga gcgtaagccc
    12721 gataccgcag tacacggcgg tttttcccgt gatcaccatg cggcgggctg cgttgaattc
    12781 gagattgaaa aaaggaagcg tgctgtacgt gacaccgcct ttgaaatcga aaaaccattt
    12841 gcgggtaaag ttgctccggt agccgatctg tttttgcagc gggtaaagcg tcagcgcctt
    12901 tcgcaggccg gtggaatctt tctgtgcagg aagaatcccg gctgtgaaaa gcagcagcag
    12961 gacgatcgcg gttcttttca ttatcgtagt tttgattttc aaacggattg ctgatcacac
    13021 gaatatacga agcggagcag tgacgatctt tttcatcggg aatggacagg aaaaaatatg
    13081 cagcgattgt aaaggaagaa gcccggcgtg ccggtttcga ttattgcgga atttcgaaag
    13141 cggaatttct cgaagaggaa gctccgcgtc ttgaaaagtg gctgagagaa ggaatgcagg
    13201 gagaaatgag ctggatggaa aatcatttcg aactgcgcct cgatccgtcg aagctcgtgc
    13261 ccggtgcgaa aagcgtgatc tcgcttttgc tgaattatta cacggagaaa aagcaggagg
    13321 acccggctgc gccgaaaatt tccatgtacg cgctgggcga agattatcac ttcgtgatca
    13381 ggcgaaagct gaaggaattg ctgaacgcgc tgcaggaaaa gatcgggaag gaaataagcg
    13441 gaagggtgtt cgtggattct gcgcctgtga tggacaaagt gtgggcgaag aaaagtggtc
    13501 tcgggtgggt ggggaaaaat tcgaacctca tcacccggga aatgggctcg ttctttttta
    13561 ttgcggagct gatcgttgac atcgaactgg aacctgattc tcccaccggg gatttttgcg
    13621 gaacgtgcac gaaatgcatt gatgcatgcc cgacggatgc gatcgtttca ccgtacgtgg
    13681 tggatggcag caaatgcatt tcctatttca cgatcgagct gaagaacgcg atccccgaaa
    13741 gtgaaaaagg gaaattcgaa aacaatatgt tcggctgcga catctgccag gacgtttgtc
    13801 cctggaaccg tttttcgcgc ccgcatacga cgcccgaatt cgggccggac gagcagcttc
    13861 tttcaatgaa aaaaagggaa tgggaagagc tcacggaaga cgtcttcgat gaaatattcc
    13921 gtcgctccgc ggtgaagcgg acgaaatttg caggactgaa aagaaatatc aggttcctta
    13981 agtcttcgtg acgctgcggt aaacgtcgcg gatgacgtct ccgtacatgt ggccgaaagt
    14041 ggcgatgcac catgccttga gcagcagcgc ttcatcgggt ttgacccact tcagcatttt
    14101 ggaaagctct ttccggaaaa gcgacttgtc aaaacttacg cttttaagaa tatgctggga
    14161 gaattctaac ataaccctac tcctttctga attgtttaac aagtaacgca aagaggaata
    14221 accaatttat aaaagcgatg ttaattttcc taaagaaaaa tggcccgaaa ccctaattgg
    14281 ttgtgattca gtaaacttag taattcaccg ccgggccggc atggtttccc gggatgtttc
    14341 agcccgttct ttacgcgttt tcattccagc gaaacgtctt tacttcgagg ccggcctggt
    14401 cgagaatgcg gtccgtgacg gtggcggcaa gttcttcaaa ggacgcgggt ttactgtaga
    14461 acgacgggct tgcaggataa acgatggcgc ccgcttcggt cactgttttc aggttgttga
    14521 tgtggatgag gctgagtggc gtttcgcgcg gaacgaggat gagctttcct ctttccttga
    14581 gcataacatc ggcggcgcgc gtaacgaggt cgttggaaat tcccgttgcg atgcgcgcga
    14641 gcgttcccat gctgcacggg cagacgatca tcgtgcggta ggaagagctg cctgaagcga
    14701 atggtgcgct gaaatcattt ttgccgaacg tgcggaaagg atatttttca tacgacgtgt
    14761 ttcccagttc cgtcttccac acttctttcg cgttgtcgct gatgagcagc gaaatttctt
    14821 ccacctgctc tttcagtgaa acgagccggt cgagcaggac tttcgcataa atggatccgc
    14881 tggcgccggt gacggcaatg acgattttct gttttttcat tttttactgc gaagtaccgc
    14941 tgtcgccgaa acggtacgcg agcgtcagcc cgagcacatt gtggtacatt cctttgttga
    15001 acaggttcat gaaaaagttc gggtaataga gcgggacctg gaatttcagc acgggcgcga
    15061 cggagttcgt gtagcggaga tcgactgaaa aatgcgggtt caggtttatt cctgcgccga
    15121 aaatgaatgc cgtttcccat ttgcggaaac cggccggatg tatttcgccg ttgctgtccc
    15181 attcacggac gttgaccagg aaaccgaagg tctggccgat ctcgaaataa acattgcgcg
    15241 aattcatgcg cagcaggaaa ggaacttccg cataattcat cgcgagcctg aaggaagtat
    15301 aatcgccttt gtccgggttg ggatttttcc ggctgccttt ggaagaatac tgcatttcca
    15361 tctgcgcata aaaacgtttt gtgggatccg tgcagacgaa aattccaccc acgacgccgg
    15421 ctttgttgaa accggaataa gaatcgccgt gcacctgcga gccgtttatg ccggcctgta
    15481 ttcctgcgcg gaacgtttgc tggctgtgcg cacgtgaaag aaaaagaagg gacgcgaaga
    15541 gaaaaaatgc aaaacctttt tttcgcatga ggtaaaatta ggcaataaaa aaacgcgccg
    15601 cagcgcgttg tattacggta aaagcagctg ttactccacg tccgcctccg ccgcctgcag
    15661 tacgccgtag tcgtccgact tataaatgaa accgaccacc cggcaatgcg cggcgttata
    15721 cgcggcattg atcgtgaaag acgggctgct gtagctgatc ccggtatggg cgggaatggt
    15781 tccgctcagc acggtatcgc cgatgatcga accgggtgtg ttgatgcagc cacggaaaac
    15841 gtgattgaaa acatagttgg aatcgtagcc gagcgaaacg gaattgtcga gctggtagcc
    15901 ggtgaggctg tcttccacaa ggtaaagcgc gaggaggtag gttccggtcg tatctctcat
    15961 catttccccg cttacctgca cattcagtgt gtgtgaagaa gccgtgtagg tcggcgtgat
    16021 cttgaggtac gcgctcatcg gggtggaaag aatagcggcg ccggttgaag accacgtggg
    16081 cgcaggtgag ggaatattgg aaggataacc ggtgcgattg accagcccgt tgggaagcga
    16141 ccaggaagag cagccgaaaa ttgcgtcgta atcggcgtct tcagccgtaa cgcggaaatc
    16201 ctcgctgaat gcacctgccg gccatgttcc tgccggttgt gtaggttgcg caaaaaatcc
    16261 gaaatgcacc gctacgggaa cgatctgccc gggataaaga tgctcgaggc tgtcgatcac
    16321 tttcgcggct gacggacagt tgacgcaagt gtgcccggta acatcttcaa tgagaatgcg
    16381 tcgtaccgtg tcgctgaccg gtgtaacgga gctgccgccc acatacggcg gaacttcaat
    16441 ataatcgcat ccccaggaag cgaatgcgat tatagcgaga agagaaatat gctttttcat
    16501 tttcagatca gttaaagaac gtttttcaga atgagctcgt gatcgtcagc gtcagtccgt
    16561 tcgatgccgg aacaaaacgg cagacaccgc ctacgcagaa aattcctgca cgctgttttc
    16621 cgtaacctac ggcgatgcgg ttggtccctt tcaggaatcc ggcctgcacg ttgtaataat
    16681 gaagacgctg gtccgcaacc gggttcccgt aattccattc gtccattcct gcaataaaaa
    16741 aatgattgcc gatcgaaact tcttccagca gcatggccca gttgccctgg tcctgcacaa
    16801 cgccgttggt ctttcctacc gacatgtgct ctgcatcggt gtggaacgtg aagtgcttgt
    16861 ttactttcca ggtcacatcc accacgccga tattcgcgtg tacgacggca taattgacgt
    16921 gaccttcgat cacgttcttg ttgtagatca tgttcgcata catcaccgtt actttcagca
    16981 ggcttgcggt gcgatgaaaa aattcacggt agatctcgat gttcgcatcc tggtaaaaca
    17041 gcgaatcgcc gacagcgctc cattttgccg agtagccgta atcttttccg agccggtcgt
    17101 tcagctgggt ggtatcgatc ccgtgcgcga cggaataatt cagcgtgatc gtcgttccgt
    17161 attttccgcc cagctttgtt ccttttctta ttttgtaatc cgcttcaccc tgccagcaga
    17221 cttcaccgtt cggctgcgaa gcgtacgggt aaaacgcggc aagaagatac gtatgctgtt
    17281 tcgtcagcgc agggagataa ttgatcatga gtgaatttcc cgttgcgtcg cggtcggagc
    17341 gataaccgaa attgtcgacg tacatgtacg cgccgtaaag cccgaagcct ttctgcgaat
    17401 aggtcgcaga ggtgaaaagc gcctggcccg gcttgtagat gtacccgttg ccgatcgtcg
    17461 gatcgttgat cttgtaggcg tattcgccgt agaggttgat cctgctggtg atgatatttc
    17521 cgcgcgccgc ccatgtgccg acgttttgcg gcaggataag cgagggattg agatcaggct
    17581 ggtatttgct gacgaagctt ccgccgaaaa tgaattgcgt tttcacgttc gccatgcagg
    17641 aatcgaaaag ttcgttgagg tttatttcgc cgtcgaaacc gcgtacaatg ccgggaccgg
    17701 tggtgaaaaa attccgttgt tttccccaga cacctttcag gtaaatgcct ttgtagggat
    17761 tgtagcgcac gcgcatgccg tcgagcgcat tgtcgtagcc gagcccgcgg tcttcgtacg
    17821 cgcgcagaac gagtccgctg ccgaactgtt cgtatgcgct gccgacggtg atttcaagtc
    17881 cgtcgttgtt atacgtggca tagcgataag gaatgcccgt tcctttgtag ccggaaggat
    17941 agccctgcat cacgttcagg tagctttcgt aacgcaggcc ggctgaaaat tttccgttga
    18001 tgtaattaat atttccccat ccgttcatca gcgctttttc cgggactgt
//
LOCUS       DASTSF010000518         4485 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1883827,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000518 DASTSF010000000
VERSION     DASTSF010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4485
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1883827"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..878
                     /locus_tag="VFU15_14850"
     CDS             <1..878
                     /locus_tag="VFU15_14850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEU4719120.1"
                     /translation="RIIASGLFLLPVFISAQSVTLSPDTALTSPSSACLACTGTTWNN
                     EGNVSLPDGLSASTTLMQNGSCFQSSCYCSRFLYANSFGFSIPLNASIDSILVDVLRY
                     ASIAGAIKDSVIQLDKSGTLVGNNLYSSAFWPATGASTTYGHSDPLWGTAPGFWTPAD
                     LNDPASGVSIKVTNLSSGQAAAYVDHIQMTVFYSTAAGTFSASSSPSRLFISSGGTDE
                     IIAITGDGKQHYSVSLMDATGKLILQQDLGIPGPGRNEFSIHLPGLVPGIYFWNLQGD
                     TENETFKTIISPQVR"
     gene            875..1216
                     /locus_tag="VFU15_14855"
     CDS             875..1216
                     /locus_tag="VFU15_14855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719121.1"
                     /translation="MKKLLPLFFLLIFGASCHHVQHTAASKDDVQAKGSGDQDGSSFD
                     NAVVIYEKTEGKGVDAEYDWLKTHYPGYTVNRQSLVTHDGKPYDVLSITTADGEKKDV
                     YFDISNFFGKF"
     gene            1228..1572
                     /locus_tag="VFU15_14860"
     CDS             1228..1572
                     /locus_tag="VFU15_14860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719122.1"
                     /translation="MKKVTPLIILLATVLFVACRTQSAATGETNTTQHANTGDQDGLS
                     FQTAVVVLDTTETKGIATEYVWMGIHYPGWHVQVQSLVFQDKKPYDVFVVKKGRKKKK
                     LYFDISNFFGKW"
     gene            complement(1573..2154)
                     /gene="coaE"
                     /locus_tag="VFU15_14865"
     CDS             complement(1573..2154)
                     /gene="coaE"
                     /locus_tag="VFU15_14865"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014680607.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEU4719123.1"
                     /translation="MKKIGLTGGIGSGKTYVAELFASLGVPVFYADAAGKQLQESDEA
                     VRRQIKDIFGEDIYSKDGKPDRKKIASIVFSDKEKLVRLNAVIHPAVANAFREFCAEN
                     SRAAYVIKEAAILFEAGADAGLDAVITVTAPLSLRTERILRRDHITKEQAEARMKNQW
                     SEEEKIRRAQFVIHNDGRLLLPQVVAIHEKLSS"
     gene            complement(2252..>4485)
                     /locus_tag="VFU15_14870"
     CDS             complement(2252..>4485)
                     /locus_tag="VFU15_14870"
                     /inference="COORDINATES: protein motif:HMM:TIGR04312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="choice-of-anchor B family protein"
                     /protein_id="HEU4719124.1"
                     /translation="DIGMSIVDVTNPSSPVEVVQIPNVNNLWKEIKVFKHYAYVTTEG
                     GGGLQIVDLRNLPGTNLPYHNYTGDGAINGQLNTIHSLHVDTAAQKVYLYGSNLFNGA
                     AVALDLSVDPYNPTYLGHYGNVYVHDGYVDNDTIFAAHIYNGYFTEANVSNLASPNVL
                     ATQTTTTSFTHNTWLSDDHRYCFTTDENTNSYLTCYDVSDPGNIQETDKIQSQNPNSG
                     SIVHNTHILHNWAVTSWYRDGFVITDITRPNNLVNVGWYDTYTSGSGNGFNGAWGVYP
                     FLPSGTIVVSNIEDGLWVFTPNYVRACYLEGTIRDSICNTPLSGVTVTIGTVNVSDVT
                     NAGGVFATGTAVPGTYNVTFSKPGYQNYVINNVTFSPGVVDTFNIKMFSSTAVALNGN
                     LSNSSTSSAIAGAQVILTSPNNTYNYVTDVNGDYSSCNVVADGYDYGIGAWGYWGMCG
                     TDSIYNGHSTQSFQLAPGYQDDFTFDLGWTVTNSSGLTTGAWVRGEPQGTTYNNPGDA
                     NPEYDVNNDCFDKCYVTGNTGTSSSDDDVDNGSTTLKSPPFDVTSYTEPWIHYSRWFF
                     NAGGSGNPNDSLIIRLTNGTTTVTVETVTASTPGMSSWVNKAFRISDYMTPSANMRLL
                     VYTADVNPGHLVEAGFDNFYILDSVTSGIHTPPAAVSGVNVYPNPFTGATQVSYTLKN
                     APSANSFMTVTDVTGRVVFTQQLVDQNGTIQLGSDLADGIYFVRVTDGAEASEAVRLV
                     KTH"
BASE COUNT         1022 a         1180 c         1214 g         1069 t
ORIGIN      
        1 aaaggataat tgcctccggc ctgttcctcc tgccggtttt tatttccgcg cagagcgtta
       61 ccctttcgcc ggatacggca ctgacgagcc cgtcttccgc atgtctcgcc tgtaccggca
      121 cgacctggaa caatgaagga aatgtatcgt tgcccgacgg gctatccgct tccaccacgt
      181 taatgcaaaa cgggtcctgc ttccagagct cctgttactg ctcccgcttt ctctacgcga
      241 attcattcgg attttccatc ccgctgaacg cgtcgatcga ttccattctt gtcgatgtgc
      301 tgcgttacgc ttccattgcc ggcgcgataa aagattcggt gatccagctc gacaaatccg
      361 ggacgctcgt gggaaacaat ctttattctt ccgccttttg gcctgcgacc ggcgcttcca
      421 caacatacgg gcacagcgat ccgctctggg gaaccgcacc gggtttctgg acacctgcag
      481 atctcaacga tccggcttcg ggcgtttcca ttaaagtgac gaacctttcc tccggccagg
      541 ctgcagcgta tgtcgatcac atccagatga cggtttttta ttcaactgca gcaggaacgt
      601 tcagcgcgag ctcttcgcct tcccggttgt ttatttcttc cggcggaacg gatgaaataa
      661 tcgcgatcac cggagatgga aagcagcatt actccgtttc gctaatggat gcaacgggga
      721 aacttatcct tcagcaggac ctcggcatac ccggtccggg acggaatgaa ttttccattc
      781 acctccccgg tcttgttccc gggatttatt tctggaacct gcagggcgat acggagaacg
      841 aaactttcaa aaccatcatt tccccacaag tgcgatgaaa aaattactgc ccctgttttt
      901 ccttctcatt ttcggagctt cctgtcatca cgttcagcat acggcagcgt caaaggacga
      961 tgtgcaggca aagggaagcg gcgaccagga tggaagctct ttcgataatg cggtcgtgat
     1021 ctatgaaaaa acggaaggga aaggcgttga tgcggaatac gactggctga aaacgcatta
     1081 tcccggctat acggtgaacc ggcaatcgct ggttacgcac gacgggaaac cgtacgacgt
     1141 gctcagcatc acgacggctg acggagaaaa aaaagatgtt tattttgaca tctcgaattt
     1201 tttcggaaaa ttttaattca acccgatatg aaaaaagtaa ccccgcttat tattctgctg
     1261 gcaacagttc tgtttgttgc gtgccggacg caatcggcgg caacaggcga gacaaacacc
     1321 acgcagcatg cgaataccgg cgaccaggac gggctttcct tccagaccgc ggtcgttgtg
     1381 ctcgacacga cggaaacgaa aggaattgca acggaatatg tctggatggg cattcattac
     1441 ccgggctggc acgtgcaggt gcagtcgctg gtcttccagg acaaaaaacc gtacgatgtt
     1501 ttcgttgtca agaaaggcag gaaaaagaag aagctctatt tcgatatttc gaatttcttc
     1561 ggaaagtggt gatcaggagc tgagcttttc gtgaatggca accacctgcg ggagcagcag
     1621 ccggccgtca ttgtgaatga caaactgcgc ccgccggatc ttttcttctt cgctccattg
     1681 atttttcatg cgcgcttccg cctgctcttt cgtgatgtga tcgcgccgga gaatacgttc
     1741 cgtccgcagc gaaagcgggg ctgtcacggt aatgacggca tccagcccgg catccgctcc
     1801 cgcttcaaaa aggatggccg cctccttgat cacgtaagcg gcacggctgt tttcagcaca
     1861 gaattcccgg aatgcgttcg ccacggcagg atgaataacc gcattgagcc ggaccagctt
     1921 ttccttatct gaaaaaacaa tcgacgcgat ttttttccgg tcaggctttc catctttcga
     1981 atagatatct tccccgaaaa tatctttgat ctgccggcgt accgcttcat ccgattcctg
     2041 cagctgcttt cctgcggcat cggcataaaa aacgggaacg cccagcgaag cgaagagctc
     2101 cgccacataa gttttcccgc tcccgatacc gccggtcagg ccgatctttt tcatgcagca
     2161 aaagtaggag atgtcaggca gcttcacgct tgaaaaaaag ccataaaaaa aggccggaca
     2221 agccggcctc tcatttcata tccggaaagc gtcagtgcgt tttcacgaga cgtaccgctt
     2281 cggaagcttc cgctccgtcc gtaacgcgca cgaaataaat tccgtcggcg agatcgctgc
     2341 cgagctggat cgtgccgttc tggtcaacga gctgttgtgt aaacacaacg cggcccgtta
     2401 catccgttac cgtcatgaag ctgttggcag aaggcgcgtt cttcagcgtg tagctgacct
     2461 gcgtggcgcc cgtgaacggg ttcgggtaaa cgttaacgcc ggaaacagca gcgggcggtg
     2521 tgtgaattcc gctcgtcacc gaatcgagga tgtagaaatt atcgaacccg gcttccacga
     2581 ggtgcccagg gtttacatcc gccgtatata cgagcaggcg catgttcgcg gaaggagtca
     2641 tgtaatcgga aatgcggaac gctttgttca cccaggagga cattcccggc gtggatgccg
     2701 tcaccgtttc aaccgttacc gtcgttgttc cattggtaag acgtatgatc agcgaatcgt
     2761 tcgggttgcc ggatcctccg gcattgaaga accagcggga ataatggatc cacggttccg
     2821 tataagacgt aacatcgaac ggaggtgatt tcagcgtggt ggaaccattg tcgacatcgt
     2881 catcggacga gctcgtgccc gtgttgccgg tgacataaca tttatcgaag caatcgttgt
     2941 tcacgtcgta ttcgggattt gcatcgcccg ggttgttata ggtggttccc tgcggttctc
     3001 ccctcaccca tgcgcccgtc gtaagaccgg aagaattggt caccgtccag ccgagatcga
     3061 acgtgaagtc gtcctggtaa ccgggagcaa gctggaagga ttgcgtgctg tgcccgttgt
     3121 aaatgctgtc ggttccgcac attccccagt aaccccacgc gccgatcccg taatcatatc
     3181 cgtccgcgac aacattgcag ctgctgtaat cgccgttcac atcggtcaca taattgtacg
     3241 tgttgttcgg gctcgtcagt atgacctgtg caccggctat cgcggaagaa gtggaagaat
     3301 tggaaaggtt tccgttgagc gcaacggcgg tggacgaaaa cattttgatg ttgaacgtat
     3361 cgacaacgcc gggcgagaac gtgacgttgt tgatcacgta attctggtag ccgggcttcg
     3421 agaacgtgac gttgtaagtt cccggaacag ctgttcccgt tgcaaaaacg ccgcccgcat
     3481 tcgttacgtc gctgacgttc accgttccga tcgtaaccgt tacacccgaa agcggcgtat
     3541 tgcagatcga atcgcggatg gttccttcga ggtagcaggc gcggacataa ttcggcgtga
     3601 acacccagag accgtcttcg atattcgaaa caacgatcgt tcccgaagga aggaaaggat
     3661 aaacgcccca cgcgccgttg aaaccgttgc cggaaccgga agtgtacgtg tcgtaccagc
     3721 cgacgttgac caggttgttc ggacgtgtaa tgtcggtgat gacgaaaccg tcgcggtacc
     3781 atgaagtgac ggcccagttg tgcaggatgt gcgtgttgtg aacgatggaa ccagaattcg
     3841 ggttctgcga ctggatcttg tctgtttcct ggatgttgcc cggatcggaa acgtcataac
     3901 acgtgagata agaattcgtg ttctcgtccg tggtgaagca ataccggtga tcatcgctca
     3961 gccatgtgtt gtgcgtgaac gaagtcgtgg tggtttgcgt ggcgagcacg ttggggcttg
     4021 cgaggttgct tacgttggct tcggtaaaat aaccgttgta aatgtgtgca gcgaagatcg
     4081 tgtcgttatc cacgtagcca tcgtgaacat aaacgtttcc gtaatgcccg agataggtcg
     4141 ggttgtacgg atcaacggaa agatcgagag cgacggcggc accgttgaat agattggatc
     4201 cgtagaggta aaccttctgt gccgccgtgt ctacgtgcag cgaatggatc gtgttcagct
     4261 gcccgttgat cgcaccatcg cccgtgtaat tgtgatacgg gagattcgtg ccgggaagat
     4321 tgcgcaggtc gacgatctgg agtccgccgc cgccttccgt ggtaacgtac gcataatgct
     4381 tgaacacttt gatctctttc cagagattgt tcacgttcgg gatctgcacg acctccaccg
     4441 gggaggaagg attggttaca tcgacgatgg acataccgat gtcct
//
LOCUS       DASTSF010000519         3918 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1918833,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000519 DASTSF010000000
VERSION     DASTSF010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3918
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1918833"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            99..2759
                     /locus_tag="VFU15_14875"
     CDS             99..2759
                     /locus_tag="VFU15_14875"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEU4719125.1"
                     /translation="MKKFFFLLSIMGTAALRGQTGLTSFAFPAGTSSHTCIASDTAGN
                     IWVGTSGNGIKKFDGTNWSYFSTTNTPMQSNNISAIGIRDGHDVWVGNAATPTSGYLY
                     LYDGVTWSDHSADVGNTLVTCIAQGNSPAEMWFGTMSGLYKFDGTGWTHFTVANSGLA
                     SDTVFALAPDNSGNVMAGTQRGLSVQNGSAWTNYNSQNSGFPSDIIKSVYCDNANGTW
                     ISTSAGAGAFWGNTFTAAGEIYPSSSSCYPTNSRNLCKGPAGGVLFTGASTSGAGLMI
                     EFKGNFPTAYSFPAPVSLSNLMLLTYNAADSCIWMTNHPGSSPLYRFDCRNYHQLAGG
                     TTPVNSRFLDINNVKASVLNRGDMHWDGQYTNEYEVPKGGGAGTIFASALWMGGLDNG
                     GGLHEAAMTYRQSGMDFFPGPLDTISGTTDTATAIQYDYMWKIDKTMILDFQYYWSTG
                     AVQNGTWVPPHDILTWPAQGNGNYTRDMAPFVDVNHNGIYDPLSGGDYPDIKGDEMLY
                     CIFNDNLSVHTNTGGQPLKVEVHFSAYSYFCPNVADSEKVINYTTFYNYKIFNRSGSG
                     YHNVYLGIFQDCDLGAYDDDHVGCFPPGNFSYCYNDTIDGWSAAPVQGTYGAHPPISS
                     IAVLDGPIAAAGDGIDNDNDGTTDEGGEKNLMSQFRYYENDFTATGNPAFAPDYYNYT
                     SGRWLDSTQVTYGGTGYGGTTPTAFMFPGWPFDTSSTAWSEIKDNDVPGDRRMLIGIG
                     PFDLAAGTSVNMDYADVFSRDTTLSPNDTQYFYQAWRDVNRIRNWYANSNAPSCVDWN
                     ASLNENANPALQFSLYPDPSASVVTLAYTPRNTNASYAIFDISGRIVEKGTVGAAGKT
                     YIGVQQFSPGIYFIRITDGGSTGTMKFIRE"
     gene            2777..3247
                     /locus_tag="VFU15_14880"
     CDS             2777..3247
                     /locus_tag="VFU15_14880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015808731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU4719126.1"
                     /translation="MQSEINIRPAVAADMPAVLALIKELAEYERAPEEVTVTAEDLVR
                     DGFGECPRFHCFVAEENKKVTGIALYYISYSTWKGSCLFLEDIVVTQSERRRGIGKML
                     FEEVIRAAKKMNAKRMSWQVLDWNEPAIKFYEKYNPEILREWLNFRLTEEKLKK"
     gene            complement(3494..3834)
                     /locus_tag="VFU15_14885"
                     /pseudo
     CDS             complement(3494..3834)
                     /locus_tag="VFU15_14885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005821902.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
BASE COUNT          937 a         1055 c         1011 g          915 t
ORIGIN      
        1 ggcttgcgga tcttggaaaa actgcgacgc tcttgttttt gaaggtccat tttgtacttt
       61 agcctctcaa tcccatcccc gcttaaatct tattcaccat gaagaaattc ttcttcctgc
      121 tttccattat gggaacagca gcactgcggg gacaaaccgg actgacgagc ttcgcctttc
      181 ctgcaggaac atcgagccat acctgcatcg cgtccgacac ggcaggaaat atttgggtgg
      241 gcacctccgg caacgggata aaaaaattcg acgggacgaa ctggagctat ttcagcacca
      301 cgaacacccc gatgcagagc aacaacattt ccgcgatcgg catccgggac ggccacgatg
      361 tgtgggtggg aaatgcggcg acccccacaa gcggttacct ctacctgtac gatggcgtca
      421 cgtggtcgga tcattccgcg gatgtcggca acacacttgt cacgtgcatc gcacagggaa
      481 attctcccgc ggaaatgtgg ttcggcacga tgagcggttt atacaaattc gacggcacag
      541 gctggacgca tttcaccgtg gcgaattccg gtctcgcttc cgacaccgtg tttgcgcttg
      601 cgccggataa ttccgggaac gtaatggccg gaacgcagcg cggactttcc gtgcagaacg
      661 gaagcgcgtg gacgaattac aattcacaga actcgggatt tccgtccgac atcatcaaat
      721 ccgtttactg cgacaacgcc aacggaacct ggatatcgac aagcgcgggt gcaggcgcat
      781 tctggggcaa cacgttcact gcggcggggg aaatttaccc gagctcgtcc agctgttacc
      841 cgaccaattc gaggaacctg tgcaaaggtc ccgcaggcgg tgttcttttc accggcgctt
      901 ccacgtcagg cgcaggcctg atgatcgagt tcaaagggaa tttccccacg gcctactcat
      961 tccccgcacc cgtttccctg tccaacctga tgctccttac atataatgca gctgattcct
     1021 gcatctggat gacgaaccac cctggttctt cgccgctgta ccgtttcgat tgccgcaatt
     1081 atcaccagct ggccggcgga acaacgccgg ttaattcgcg ctttctcgac atcaacaacg
     1141 tgaaagcatc cgttctcaac cgcggcgaca tgcactggga cgggcagtac accaatgaat
     1201 acgaagtacc gaaaggcggc ggcgccggaa cgatcttcgc ctctgcctta tggatgggcg
     1261 gactcgacaa tggcggcgga ttgcacgaag cagcgatgac gtaccgccag agcgggatgg
     1321 attttttccc cgggccgctc gatacgatca gcggaacaac agatacagcg acggcaatac
     1381 aatacgatta tatgtggaag atcgacaaga cgatgatcct cgatttccag tattactgga
     1441 gcaccggcgc cgtgcagaac ggaacgtggg ttcccccgca cgacattctc acctggcccg
     1501 cgcaggggaa cgggaattac acgcgcgaca tggcgccttt tgtcgacgtc aatcacaacg
     1561 gcatttacga tccgctctcc ggcggcgatt atcccgacat caaaggtgac gagatgctgt
     1621 attgcatctt caatgacaat ctctccgtcc acaccaatac cggcggacag ccgctgaaag
     1681 tggaagtgca tttttcggcg tattcttatt tctgccccaa tgtcgcggat tcggaaaaag
     1741 tgatcaatta caccacgttc tataactaca aaattttcaa ccgcagcggt tccggctatc
     1801 acaacgttta tctcggcatt ttccaggatt gcgatctcgg cgcttacgac gatgaccacg
     1861 tgggttgttt tccgcccgga aatttcagct actgctacaa cgataccatt gacggctgga
     1921 gcgccgcccc cgtgcaggga acctacggcg cgcatccgcc catctcgagc attgcagttc
     1981 tcgacggacc aatcgcggct gccggtgacg gcatcgacaa cgacaatgac ggaacaaccg
     2041 atgagggcgg ggagaaaaac ctgatgagcc agttccgtta ttatgaaaat gatttcaccg
     2101 ctaccgggaa cccggccttc gccccggatt attacaacta cacttccggc cggtggctcg
     2161 acagcacgca ggtgacgtac ggcggaacag gctatggcgg aacgacgccc accgctttca
     2221 tgttccccgg ctggccgttc gatacgtcgt cgaccgcgtg gagcgaaata aaagacaacg
     2281 atgtgcccgg cgaccgtcgg atgctcatcg gcatcgggcc gttcgatctt gctgcgggca
     2341 cttcggtgaa tatggattac gccgacgttt tcagtcgcga cacaacactt tcaccgaatg
     2401 acacgcagta tttctaccag gcgtggcgcg acgtgaaccg catacgcaac tggtacgcga
     2461 attcgaacgc gccttcgtgc gtcgactgga acgcatcgct gaatgaaaat gcgaacccgg
     2521 cgctgcagtt ctcgctttac ccggatcctt ccgcatccgt ggtcacgctc gcatacacgc
     2581 cgcggaatac gaacgcttcg tatgcgattt tcgacatcag cgggaggatc gttgaaaaag
     2641 ggaccgtcgg cgctgccggg aaaacatata tcggtgtgca gcagttttcg cccggcatct
     2701 atttcattcg catcacagac ggaggcagca caggcacgat gaagttcatc cgcgagtaac
     2761 tgcgctatct ttgcacatgc agtcagaaat aaacatacgg ccggccgtcg cagcagacat
     2821 gccggccgtt cttgcgctta tcaaagaact ggccgaatac gaacgcgcgc cggaagaagt
     2881 taccgtcact gccgaagacc tcgttcgcga tgggttcgga gaatgcccgc gctttcactg
     2941 cttcgttgcg gaagaaaaca aaaaagtgac cggcatcgcg ctttactata tcagctattc
     3001 cacgtggaaa ggaagctgcc ttttcctcga agacatcgtc gtcacgcaaa gcgaacggcg
     3061 gcggggcatc gggaaaatgc tgttcgaaga agtgatccgc gctgcgaaaa aaatgaacgc
     3121 gaaacgcatg tcgtggcagg tgctcgactg gaatgaaccg gcgataaagt tttatgaaaa
     3181 atacaatcct gaaattcttc gcgaatggct gaatttccgt ttgacggaag aaaaactgaa
     3241 gaaatagcag gatgctccgc aggcaaccgt ttctgtcgtc cgcttgttgc aaccaatgtc
     3301 ggtgtaaagt ctttgggtca ttgttgcgtt gtgcgttcat gtttttaaag cttcgggaga
     3361 gaaggaaagt aaaaaataat tttttccggg ctgtaaaccg ggaagctctt tgcaattttt
     3421 ggttcgtgtg ccggttggtg tgaattgtaa atgtgcttat cccggtagtt atcaggatgt
     3481 gctggtctat tccttattcc ataattgatt ttacctgttt ctgtaatagc tttctgtccg
     3541 gtaaatacaa ctgatatttt gaaacgaata ttttatttga aatcccgcct gttgcatact
     3601 ctgccaacac ttcgtttttg tctgcagaca gaatgatgcc gatcggttca ttgtcgcctc
     3661 cacgctttcc tccgtcttaa agtaattcag gtaaaggttc atttgcccga tgtcgttatg
     3721 cctgaccggt tttgttttca ggtcgatcag cacaaaacat ttcaggatgc ggtgataaaa
     3781 taccaggtca atgtaaaagt gtttgttgcg cagcgaaatt ttatactgcc tgccattttg
     3841 gataggccag gatcagggca ttgaaattcc tgcattaagt tttcaatatg ttcaccaggc
     3901 atatttagcc attttccc
//
LOCUS       DASTSF010000520         8129 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_241990,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000520 DASTSF010000000
VERSION     DASTSF010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 8129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8129
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241990"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..672)
                     /locus_tag="VFU15_14890"
     CDS             complement(<1..672)
                     /locus_tag="VFU15_14890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015333614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU4719127.1"
                     /translation="MITVSNLTLQYGKRVLFDEVELKFTPGNCYGIIGANGAGKSTFL
                     KILSGEIDPTKGSVSITPGLRMSVLKQNHFEFDEFPVLQTVIMGNTKLYKVMQEKDAI
                     YAKTDFSEKDGMRASELEAEFAEMNGWNAESDAADLLSKLGIREELHGRLMNELSGKE
                     KVRVLLAQALFGNPDILLLDEPTNDLDVETISWLEDFLADYPNIVIVVSHDRHFLDAV
                     CTHVCD"
     gene            complement(804..1304)
                     /locus_tag="VFU15_14895"
     CDS             complement(804..1304)
                     /locus_tag="VFU15_14895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719128.1"
                     /translation="MISRQQLIDSLVKECNVSKHLFSRIPTDAYDFRPTEKQRSTLEL
                     LRYLAYCGSAPIHVMLNGSDWKTQWKPYTESVARMRPEEFPAAMDRQAEEMTRMINSI
                     PDEDFTTKEVKHPTGEVMTLGLGLVRMPLSWLTAYRQQLFLYAKQSGAHELGTSNNWH
                     GRDRQV"
     gene            complement(1375..6225)
                     /locus_tag="VFU15_14900"
     CDS             complement(1375..6225)
                     /locus_tag="VFU15_14900"
                     /inference="COORDINATES: protein motif:HMM:NF012271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HEU4719129.1"
                     /translation="MIRIFSFSSGCRRLCAVAAVVLAAFCVKAQSVANYAVTRTTGIT
                     YSSIMSTGVPCNSWRNSATFEEDDNRSNPVDIGFDFWYDGTRYTSLCISTNGYIDFSS
                     TTDNGGPTTGPYGYSNTQFTAPNGTLLALAPFYDDMTTQGATDPLGNSIRTQLSGTAP
                     NRVFTIEWFDMAVYLNTTPSLNFQVKLHETTGIIEFVYGTMNAGTSGFSYTCGINAAT
                     MNNTPTTAQLKCQQTANTTTFSNGEQNNLSVMPTSNSKLTFTPPAPANPSGSLTFSGV
                     TQTQMTLNWTNWATNEVGYVIYSSTDNVNWNFETQTAANATSATITGLYSGTTYYWRV
                     YAVTEGWLSNALSGSHATLNGTIYISISSGNWSQGTIWNTGTAPGANDDVIIGNGTTV
                     TTNLANLGCHNLTVGQGAAATLRFGNNNVARTFTVGGNITVNSNATLTVNTSSNTTHT
                     LIVSGNVTNNGTWNMSPDNNSHCNVSFTYATANQAFQGSGTTNNFYNITIDKGSEDTR
                     IFEIKTSTFTAPAGFLTLNNGTFKVSTTGAVSMTPFTATSDIPDHARFWLNSSTATVT
                     TTGGSINLYGELMITAGTMTLGNASDDNLISQGGLFTMNGGTMNIAGRYDRSNTATLS
                     RFIITGGTLTINTVGSTSNTNAPFMMDVIGSQFTQTGGTIIIQRKGGTTGNLGFNCTG
                     GVINSVTGGVLQIGNASTPVSQTITVNTVSPVGAFRVASANATALLATNPLTVINDVE
                     LQSGVFNTANLNVNVGGNWNNTGGTYTPGTNTTTFDGTTQTISRTSGTEMFNHISFTG
                     SGTKTLACAINCKNVTIAAGSTFSAGATGFAIGTTGNWSNAGSFSAGTAGVVTFNGTA
                     AQTIGGTAVTVFRDLTIQNSAGVSITANESIKGTLLLNSGTFTTTGHSFTLISDASGT
                     ARIGTITGGNITGNITMQRYIYLGPTNWRQVCAPVTGNTLQGWNDNIITAGFPGSDYP
                     NMNGWYSIGTYDETVAGPKENGYSPPSNITDALTAKRGYYVYIGPLPVTLDVTGPPVK
                     MNQTFTLTYTPSAGPTQDGWNMLGNPYPSSIDWDAAGFVRTNTDNVLYVWNPSLNQYA
                     TYVGGVGVNGGSRYIPSSQAFWVRAIAAGPSVSINEASKAGQDQPFMRMGPAEYAQQQ
                     SSNQLLGLTLSGANGTDETVIRFNAAATDSFDVNLDAYKFGSMDTTAPYFASVIPSQE
                     DLAVNSLPDVTTNISIPLRVRTGASGTYTIARDSMTNVPNSMCVTLEDLYNGTVTPFP
                     AGATYSFFLSDTTRAPRFLLHFGPSLETGSIASLCMSSNDGKAFARGTGNGPWDYTWK
                     DASGNVIATHNNISGTDTLFGAGAGVYTVEVNGNDGFCTFRSDTVRVDAPQAIVAYGS
                     IYAPSCSYTNDGRLYVNSVSGGTAPYTHYWNASNTTDSLVNIPQGNYYLHVTDANGCA
                     DSTLFTLLPLSQLNAAFVAMPDTITPLSPVSFSNYTSGGTNYSWNFGDGSPADATPDP
                     YYSYGAPGSYTVVLVAEDSLCSDTAMSLVYVMDNTGIATHGQSSDVSFAAGNSSIGVI
                     FHLPETESALIDVYDASGKLIAEVKQDQFDGRVDIPVPGIATGMYSVIVTLPGRTVAG
                     KVIFRK"
     gene            6575..>8129
                     /locus_tag="VFU15_14905"
     CDS             6575..>8129
                     /locus_tag="VFU15_14905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719130.1"
                     /translation="MSRKILTAIASVVVAVMIAIPFIAPRGKTEVQATKVDPAFGAYI
                     SAYTSGIISDESYIRITLASEYTGPVTLDKPADEDYFSFDPGIPGKTYWLDTRTLEFR
                     PDARLASDKVYNCEFRLGKLITDIPEQFRTMKFSFRTMKQGFNVSVDGMKTTDRKTMR
                     WQQMSGTLTTNDAADPKIVEQMLAVAQDNHALHVKWIHEGDNVTHHFTVDSIARRDRE
                     GKVTLTTDGKALGVEQKNSSDIKIPSLKDFKVTDVRLEQGEEQSVVVEFSDPLQEKQN
                     LDGLVTLKGVDNLRFSIEDNDVRIFPPSQLSGDYTLTVTHGVKNILGFSLPNSFTQVM
                     TFEELKPQVKLVGKGVILPNSKAGLLFPFQAVSLKAVDVKIIRIYENNIPQFLQVNSL
                     DGSSELYRVGKVVVKKKVDLNIKSKADYSKWTSFSLDLGEMIKAEPGAIYRVSIGFRK
                     SYAVYNCAGDTTTNSTIANDDEDNSFSGGDDDEDDDNGYGYYGGDYYDDEYYYYDDGD
                     RADPCDDSYY"
BASE COUNT         1801 a         2193 c         2225 g         1910 t
ORIGIN      
        1 gtcgcataca tgcgtgcaca ctgcatcgag gaagtgacgg tcgtgcgaaa cgacgatgac
       61 gatgttggga taatcggcga ggaaatcctc gagccaggaa atggtttcca cgtcgaggtc
      121 gttcgtcggc tcgtcgagca ggagaatatc cgggttcccg aacagcgcct gtgcaagcag
      181 gacgcgcact ttttcctttc cgctgagctc attcatgaga cggccgtgca gttcctcgcg
      241 gatgccgagc ttgctgagca ggtcggcggc atcgctttcc gcattccacc cgttcatctc
      301 ggcgaattcc gcttccagct ccgaagcgcg catgccgtcc ttttcggaaa aatccgtttt
      361 cgcgtagatc gcgtcttttt cctgcatcac tttgtacagc ttcgtatttc ccatgatcac
      421 ggtctgcagc acgggaaatt catcgaactc gaagtggttc tgcttcagca ccgacatccg
      481 caatcccggc gtaatgctga cggagccttt cgtgggatcg atctcgcccg aaaggatctt
      541 caggaacgtg gattttcccg caccgttcgc accgataatg ccgtaacagt tgcccggcgt
      601 gaatttcagt tcgacttcat caaaaaggac acgcttgccg tattgcagcg taagattgga
      661 tactgtgatc atgattcccg gaaatgaagc ggcaaagata cgaatgcagc agcttcttct
      721 ttaaaaaaca ttcgccaaga caataacagc tcccttcgca aaaaaggcga ttcaaatgca
      781 ccggcaaaaa agaattccgg accttacacc tgccggtcgc gaccgtgcca gttgttggat
      841 gtgcccagct cgtgtgcgcc ggactgcttg gcgtaaagaa aaagctgctg acgatatgca
      901 gtgagccagc tgagcggcat gcgcacaagc ccgaggccga gggtcatgac ttcacccgtc
      961 ggatgcttca cttctttcgt ggtaaaatct tcatcaggaa tggaattgat catgcgcgtc
     1021 atctcttccg cctgccggtc cattgctgca ggaaattctt ccgggcgcat ccgggccacc
     1081 gattccgtat atggtttcca ctgcgttttc cagtcgctcc cgttcagcat gacgtgtatg
     1141 ggcgcgcttc cgcaatatgc gaggtagcgc agcaattcga gcgtggaacg ctgcttttcc
     1201 gtcggacgga aatcatacgc gtccgtggga attctggaaa agagatgctt gctcacgttg
     1261 cattctttga caagcgaatc gataagctgc tggcgggata tcatgtgaaa cagattacag
     1321 gtttgcgcga aggtacgaat tgggaaaggt tgcacatgaa tcgcaaagga ttcctcactt
     1381 cctgaagatc acttttcccg ccacggttct tcccggcaac gttacgatca ccgaatacat
     1441 tcccgtggct atcccgggaa ccggaatgtc gactcttccg tcgaactggt cctgcttcac
     1501 ttccgcgata agcttcccgg aagcgtcgta cacatcgatc agcgcggatt ctgtttccgg
     1561 caggtgaaag atcacgccga tggaagaatt tcccgcggcg aatgaaacgt cgctgctctg
     1621 cccgtgcgtg gctatgcctg tattgtccat gacatacacc aatgacatcg ccgtgtcgga
     1681 acaaaggctg tcctccgcaa caagcacaac cgtgtacgat cccggcgcgc cgtaggaata
     1741 atacggatca ggcgttgcat cggcgggcga accgtcaccg aaattccagg agtaattcgt
     1801 tccgccgctc gtgtaattgc tgaaggaaac cggtgacaac ggcgttatcg tatccggcat
     1861 ggcgacgaat gccgcgttca gctgtgaaag aggaagcagc gtgaacagcg tggaatctgc
     1921 gcacccgttc gcatccgtca cgtgcaggta ataatttccc tgcgggatgt tcacaaggga
     1981 atccgtcgta ttgctggcgt tccagtaatg cgtgtaaggc gctgttccgc cggaaacgga
     2041 attgacatac agccttccgt cgtttgtata gctgcacgaa ggcgcataaa tgcttccgta
     2101 agcaacaatc gcctgcggcg catcgacccg cacggtgtcc gaacggaacg tgcagaagcc
     2161 gtcgttgcca ttcacttcca ccgtgtaaac gcctgcgccc gcgccgaaaa gcgtatcagt
     2221 gccgctgatg ttgttatgcg tcgcgatcac gtttccggat gcgtctttcc aggtgtaatc
     2281 ccacggacca tttcctgttc cccgcgcaaa tgcttttccg tcgttgctgc tcatgcacag
     2341 cgaggcaatg cttcctgttt cgagcgaggg tccgaaatga agaaggaacc gcggggcgcg
     2401 cgtcgtgtcg gaaaggaaga aagaatacgt ggcgcccgcc gggaaaggtg tgacggtccc
     2461 gttgtaaaga tcctcgagcg tcacgcacat gctgttcggc acattggtca tgctgtcgcg
     2521 tgcgatcgta tacgttccgc ttgcacccgt gcgtacacgg agcgggatgg aaatattggt
     2581 cgtgacatcc ggcagcgaat tcacggcaag atcttcctgc gacgggatca cgctggcaaa
     2641 atacggcgct gtcgtgtcca tgcttccaaa cttgtaagca tcgaggttga cgtcgaatga
     2701 atccgttgcc gccgcgttga agcggatcac cgtttcgtct gttccgttcg cgccggaaag
     2761 cgtgagaccc aaaagctggt tcgacgattg ctgctgcgca tattctgccg ggcccatgcg
     2821 catgaacggc tgatcctgtc ccgctttcga ggcttcgttg atcgaaacgc tgggacctgc
     2881 agcaatggcg cgcacccaga acgcctgcga ggagggaatg taacgcgagc cgccattgac
     2941 gccgacaccg cccacgtacg tcgcgtactg gttgagcgaa ggattccata cgtacagcac
     3001 gttgtccgta ttcgtgcgca cgaatcctgc tgcatcccag tcgatggatg aaggatacgg
     3061 attgccaagc atgttccatc catcctgcgt cggaccggcg gaaggtgtat acgtaagcgt
     3121 gaacgtctgg ttcattttca ccggaggtcc cgttacgtcg agcgtcaccg gcaaaggtcc
     3181 tatgtataca taatatcctc ttttcgccgt gagcgcatcg gtgatgttcg acggcggact
     3241 gtagccgttc tctttcgggc ccgccacagt ttcatcatac gtgccgatcg aataccagcc
     3301 gttcatgttc ggataatccg acccgggaaa tccagctgtg atgatgttgt cgttccatcc
     3361 ctggagcgta ttcccagtaa caggcgcgca gacctgtctc cagtttgtcg ggccgagata
     3421 aatgtatcgc tgcatcgtga tgttcccggt gatgttcccg ccggtgatcg ttcctatgcg
     3481 cgctgtgccg gatgcatccg aaataagcgt gaatgaatgt cccgtggtcg tgaacgtccc
     3541 gctgttgagc agcaacgttc cctttatgga ttcattcgcg gtaatgctta cgcccgcaga
     3601 attctgaatg gtgaggtcgc ggaaaacagt aacggcggtt ccgccgatgg tctgtgcagc
     3661 ggtcccgttg aacgtcacca cgcctgctgt tccggcggaa aagcttccgg cattggacca
     3721 gttgcctgtc gtgccgatcg cgaaacctgt tgcacctgcg ctgaatgtcg agccggctgc
     3781 gatcgtaaca ttcttgcagt taatggcgca cgcaagcgtt ttcgttccgc ttcccgtgaa
     3841 tgagatgtgg ttgaacattt ccgttccgct ggtgcgtgaa atggtttgcg tcgttccgtc
     3901 gaacgtcgtt gtattggtgc ccggcgtgta agtgccgcct gtattgttcc agttcccgcc
     3961 gacattgaca ttcagattgg ccgtattgaa aacgccggac tgcagctcga catcattgat
     4021 aaccgtcagc ggattggtgg cgagcagcgc cgtggcattg gcagaagcga cacggaaagc
     4081 acccacgggc gaaacggtgt tcactgtaat tgtctgtgaa acgggcgtcg atgcattccc
     4141 gatctgcagc acaccgcccg taacggaatt gatcacaccg ccggtgcagt tgaaaccgag
     4201 attgcccgtc gttccgcctt tgcgctggat aatgatcgtg ccgccggtct gcgtgaactg
     4261 tgaaccgatc acgtccatca tgaacggagc gtttgtgttc gacgtcgaac cgaccgtatt
     4321 gatggtgagc gtgccgccgg tgatgataaa gcgtgaaagc gttgctgtgt tcgaccggtc
     4381 ataacggcct gcaatgttca tggtcccgcc gttcatcgta aaaagtccac cctgggaaat
     4441 gaggttgtca tcggaagcat tgcccagcgt catggttcca gcggtgatca taagttctcc
     4501 gtacaggttg atgctgccgc ccgttgttgt taccgttgcc gtgctgctgt tcagccagaa
     4561 acgtgcgtgg tccggaatat ccgacgtggc cgtgaagggt gtcatggaaa cagctcctgt
     4621 ggtggaaacc ttgaacgtcc cgttgttcag cgttagaaaa ccggccggcg cagtgaacgt
     4681 ggatgttttt atttcaaata tccgcgtgtc ttccgatcct ttgtcaatgg tgatgttgta
     4741 aaaattattc gtcgttccgc ttccctggaa cgcctggttc gccgtggcgt aggtgaacga
     4801 aacgttgcaa tggctgttgt tgtcgggcga catattccac gtgccgttgt tcgtcacgtt
     4861 cccgctgacg atcagcgtgt gcgtcgtgtt ggaagatgtg ttgacggtaa gtgtcgcatt
     4921 ggaattaaca gtaatgttcc cgcccacggt gaacgtgcgc gccacgttgt tgttcccgaa
     4981 gcgcaacgtt gcggcggcgc cctgcccaac ggtgagatta tggcaaccga ggttggcgag
     5041 attggtcgta accgtagtgc cgttgccgat aatgacatcg tcattcgcgc ccggcgcggt
     5101 tcccgtattc catatcgtgc cctggctcca gttgccgctg cttattgaaa tgtatatcgt
     5161 accattcagc gttgcgtgcg atccgctcag cgcattggac agccatcctt cggtaacggc
     5221 ataaacgcgc caataataag tggtgccgga atacaacccg gtgatcgttg cggaagttgc
     5281 attggccgcg gtctgtgttt caaaattcca attgacatta tccgtggagc tgtagatgac
     5341 gtaacccact tcgttcgtcg cccagttcgt ccagttgagc gtcatctgcg tctgcgttac
     5401 gccactgaac gtaagcgagc cggaaggatt cgccggggcc ggcggtgtga acgtaagctt
     5461 gctgttgctc gtgggcataa cggaaaggtt attctgctcg ccgttggaga acgtggtggt
     5521 attcgccgtc tgttggcatt tcagctgcgc ggttgtcggc gtattgttca tcgtcgccgc
     5581 gttgatgccg cacgtgtatg aaaaaccgga agtccctgcg ttcattgttc cgtaaacaaa
     5641 ttcgatgatg ccggtcgttt catgcagttt cacctggaaa ttcaggctgg gcgtggtatt
     5701 gagatacacc gccatatcga accattcgat cgtgaacacg cggttaggcg ctgttccgga
     5761 aagctgcgtg cggatagaat tgcccagcgg atcggtggcg ccctgcgtcg tcatatcgtc
     5821 atagaaaggc gcaagcgcaa gaagcgttcc gttgggtgcc gtgaactgcg tattggaata
     5881 tccgtaagga ccggtggtgg ggccgccatt gtccgtggtg gaagaaaaat cgatgtagcc
     5941 gttcgtggaa atgcagagcg aagtgtagcg cgtgccgtcg taccagaaat cgaaacctat
     6001 gtcaaccggg ttgctgcggt tgtcatcttc ctcgaacgtc gccgaatttc tccagctgtt
     6061 gcagggaacg cccgtcgaca tgatcgaact gtaagtgatt cccgttgtcc ttgtgacggc
     6121 ataattcgcc acggactggg cctttacgca aaaagcagca agcaccacag ccgctactgc
     6181 gcaaagtcgc cggcacccgg aagaaaaact aaaaattcgg atcatgaagg agttgccgca
     6241 atttctgttc tattttacga tttccccgga attctaaacc gtttttttcg acgaaaggaa
     6301 gattgagacg aatcaatgac gcccactcat gcatgggcgg atctccttcc gtgaatttcc
     6361 ggcgttaccc ggggaacccg tatgttaaat ttaactgaat tacggttccc gtccgggatt
     6421 tgttttttcc cgcccttcat ttacgttaaa tcacatacag gtattcttta ccaataacat
     6481 ttatccttcc ttttcaccat ccggataaaa acattcaaca tcaccggaat catttaatgc
     6541 tttgccatag cttcgtccta taaatttcca aaccatgtca cgcaaaatcc tcaccgccat
     6601 cgcttccgtc gtagttgcgg taatgatcgc catcccgttc atcgccccgc gcggaaaaac
     6661 tgaggtccag gccacgaagg tcgatcccgc cttcggcgcc tacatctccg cctatacttc
     6721 gggcatcatt tccgacgaat cctacatccg cattacgctg gccagcgaat acacgggccc
     6781 ggtaacgctc gacaagcctg cggacgaaga ttatttttca ttcgaccccg gcattcccgg
     6841 caaaacgtac tggctggaca cgcgcacgct tgaattccgc ccggatgccc gcctcgcgtc
     6901 ggacaaggtc tacaactgcg aattccgtct cggcaagctg ataacggata tcccggagca
     6961 attccgtacg atgaaattca gcttccgcac catgaagcag ggtttcaatg tttcggtgga
     7021 cggcatgaaa accaccgacc ggaaaaccat gcgctggcag cagatgagcg gcacgcttac
     7081 cacgaatgat gcagccgacc cgaagatcgt ggagcagatg ctggccgttg cgcaggacaa
     7141 tcacgcgctg cacgtgaaat ggatccacga gggcgataac gtaacgcatc attttacggt
     7201 cgacagcatc gcgcggcgcg acagggaagg caaggtcaca ctgaccaccg acggaaaagc
     7261 gctcggcgtg gagcagaaaa attcttccga cataaaaatt ccgtcgctga aggatttcaa
     7321 agtcaccgat gtgcgtcttg agcagggcga agaacagagc gtcgtcgtgg aattctccga
     7381 tccgctccag gaaaagcaga acctcgacgg gttggttacg ctgaaaggcg tagataattt
     7441 acgtttttcc atagaggata atgacgtccg cattttccct ccgtcgcagc tgagcggaga
     7501 ttacacgctg accgtaacgc acggtgtaaa aaatattctc gggttcagcc tgcccaacag
     7561 ctttacgcag gtgatgacct tcgaagaact caaaccgcag gtgaaacttg tcggcaaggg
     7621 cgtcattctg cccaacagca aggcgggcct cctgttcccg ttccaggccg tcagcctgaa
     7681 agcggtcgac gtgaagatca tccgcattta cgagaacaac atcccgcaat tcctgcaggt
     7741 gaattcgctc gacggaagca gcgagctgta ccgcgtcggg aaagttgtgg tgaaaaagaa
     7801 agtcgacctc aacatcaaaa gcaaagcgga ttattcgaaa tggacatcat tctcgctcga
     7861 tctcggggaa atgatcaaag cggaaccggg cgccatctac cgggtttcca tcgggttcag
     7921 gaaatcctac gcggtataca attgcgccgg cgataccacg acaaattcca cgatcgcgaa
     7981 tgacgacgag gacaacagct tttccggcgg tgacgatgat gaagatgacg acaacggata
     8041 cggttattac ggcggagatt attacgatga tgaatattat tattacgacg atggcgatcg
     8101 cgccgatccc tgcgatgatt cctattact
//
LOCUS       DASTSF010000521         2557 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1211039,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000521 DASTSF010000000
VERSION     DASTSF010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2557
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1211039"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..812
                     /locus_tag="VFU15_14910"
     CDS             <1..812
                     /locus_tag="VFU15_14910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719131.1"
                     /translation="QDHRMKRFFPLLALLIPLFAYSDPNNGTLVIEGKYQNKNIYVQN
                     SFNQNGVGFCAYEVYVNGQRTTDEVNSTAFEIDLTPYNLHQGDSVEIQIYHKDGCSPR
                     VLNPEALKPRPTFNTQSITISATGLLSWTTDGESGVLPFIVEQYRWSKWVYVGEVQGF
                     GTPGQHNYSFQVTPHSGENKFRVKQVGFGKEVKYSPEVTYTPKDKPVLTFTQSADGKQ
                     VTFSGESLYEMYDAYGNVVLKGYGTKLDLSAFDPGSYFLCFDNQIVEIKRK"
     gene            complement(862..1506)
                     /locus_tag="VFU15_14915"
     CDS             complement(862..1506)
                     /locus_tag="VFU15_14915"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HEU4719132.1"
                     /translation="MQRLFGHPSLFSETLKKKLAEKLPGEAAQNRMSSRVRMSTEEYL
                     LRFPGYRTSSVLMMFFPIGKEIGTLLIERPGYEGVHSGQLALPGGKKEPGDADDISTA
                     LRETEEEVGVHVERSAVLGKLTPLYIPPSNFLVHPFVSFLPERPQWKADPDEVADIIE
                     VPLSVLFDDKQKGRERIPVGKNLFVDAPCYLPFGKVLWGATAMMFAEMETLLKR"
     gene            complement(1524..>2557)
                     /locus_tag="VFU15_14920"
     CDS             complement(1524..>2557)
                     /locus_tag="VFU15_14920"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEU4719133.1"
                     /translation="HPKRTTLSVHIRHVSRALAIFVALFPCILFAGNGDTLALSPRAA
                     AIASSIDEMSEPELVLLMDSLIDADVREAPLYALIHRKIDEIHASIPKNNFDDGFQFY
                     PGNRFYGIWDTQHLFPYPDSLFKCDTVTDLNLAPEISGEFFFPIIGPITSPFGWRDTA
                     YHRGIDIDLNRGDSVRSAFSGMVRLAAKEGGYGNVVIVRHYNGLETVYAHLWKIKVKP
                     GDIVTSGQLLGLGGSTGHSTGTHLHFETRFRGVAINPAWIIDLKTHRLFTDKISLVRT
                     RQGYAVRPTLEDFHTVVRGDNLYTIAQHYGISVKQLRAWNGWNGPVHLKAGQQVRIKQ
                     PAEVTSSTF"
BASE COUNT          596 a          681 c          707 g          573 t
ORIGIN      
        1 aacaggacca tagaatgaaa cgattttttc cattgcttgc cctcctgatt ccgcttttcg
       61 cctacagcga tcccaataac ggtacgctcg ttatcgaagg aaaataccag aacaagaata
      121 tctatgttca gaattccttc aaccagaacg gcgtcggttt ctgcgcttac gaagtttatg
      181 taaacggcca gcgcacgacg gatgaagtca attcaactgc gttcgagata gatctcacac
      241 cttacaatct gcaccagggt gattccgtcg agatccagat ctaccacaag gacggttgct
      301 cgccgcgcgt gctcaatcct gaagcgctca agccgcgtcc cactttcaat acgcagtcaa
      361 tcaccatttc cgccacgggc cttctttcct ggacaaccga cggcgaatcc ggcgttcttc
      421 cgtttatcgt tgaacaatac cgctggagca agtgggtgta tgtcggagaa gtgcagggat
      481 tcggaacgcc gggccagcac aactattctt tccaggtgac gccgcattcc ggtgaaaata
      541 aattccgcgt aaagcaggtc ggatttggaa aagaggtgaa gtattcgcct gaagtaacgt
      601 acacacccaa ggacaaacct gtgctgacgt tcacgcaaag cgcggacgga aaacaggtga
      661 cattctccgg ggaatcgctg tatgaaatgt atgacgccta cgggaacgtg gtgctgaaag
      721 gatacggtac aaagctggat ctctccgcgt tcgatccggg ttcctacttt ctctgcttcg
      781 acaaccagat cgtcgagata aaaagaaaat aaaatatggt ttttgagaat gaaaagacgc
      841 cgctgttaca gcggcttttt tttatctctt cagcagcgtt tccatttccg cgaacatcat
      901 ggccgttgca ccccagagca ctttcccgaa aggcagataa cacggcgcat ccacgaaaag
      961 atttttaccc accgggatcc gttcacggcc cttctgtttg tcgtcgaaaa gaacggagag
     1021 aggaacttcg ataatgtccg ctacttcatc cgggtccgct ttccattgcg gtcgttccgg
     1081 caggaaagaa acaaaaggat gaacgagaaa attgctcggt ggaatataca gcggtgtgag
     1141 ttttccgagg accgcgcttc gctccacgtg cacgccgact tcttcttccg tttcacgcaa
     1201 tgctgtgctt atgtcatccg cgtcaccggg ctcctttttt ccgccgggaa gcgcgagctg
     1261 cccgctgtgc acgccttcat agcccggccg ttcgatcagc aatgtcccga tctcttttcc
     1321 gatcgggaaa aacatcatga gcaccgagct ggtgcgatag ccgggaaagc ggaggagata
     1381 ttcttccgtg ctcatgcgca cgcgcgagct catgcggttc tgcgcggctt cgcccgggag
     1441 cttttccgcg agcttttttt tcagcgtttc agaaaaaagg gaaggatgac cgaaaaggcg
     1501 ttgcattacc gcaaaggtaa cgctcagaaa gtggaagagg tgacctcggc aggttgcttt
     1561 atcctgacct gctgcccggc cttcaggtga acgggcccgt tccagccgtt ccaggcgcga
     1621 agctgcttca ctgaaattcc gtaatgctgc gcgatcgtgt acaggttgtc gcctcggaca
     1681 acggtgtgaa aatcttccag cgtcgggcgg acggcgtaac cctgccgcgt gcgcacaagc
     1741 gagatcttgt ccgtgaagag acgatgcgtt ttcaggtcga tgatccacgc cgggtttata
     1801 gcgacgcccc ggaaacgggt ttcgaaatgc aggtgcgttc ccgtggaatg tcccgtgctt
     1861 ccgccgagtc ccagcagctg cccgctcgtg acgatatcgc cgggcttcac ttttattttc
     1921 cagagatgcg cgtacaccgt ttcgagtccg ttgtagtgcc gcacgatgac gacgttcccg
     1981 tagccgcctt ccttcgcagc caggcgcacc attccgctga aggcggagcg aacgctgtcg
     2041 ccgcggttga gatcgatgtc gatgccccgg tgataggccg tatcgcgcca gccgaatggc
     2101 gaggtgatcg ggccgataat ggggaagaag aattcgccgc tgatctcggg cgcgaggttg
     2161 agatccgtca ccgtatcgca tttgaaaagc gaatcgggat aaggaaaaag atgctgcgta
     2221 tcccagatgc cgtaaaagcg attgccggga tagaactgga atccgtcgtc gaaattgttt
     2281 ttgggaatgg aggcgtgtat ctcgtctatt ttccggtgaa tgagcgcata cagcggcgct
     2341 tcgcggacat ccgcatcgat gagcgaatcc atcagcagca cgagctcagg ttcgctcatt
     2401 tcgtcaatgg acgaagcgat ggcggccgca cggggcgaaa gtgcgagcgt atctccgttg
     2461 ccggcgaaaa gaatgcacgg aaaaagagcg acgaagatcg ccagcgcgcg ggaaacgtgc
     2521 cgaatgtgaa cagacaatgt cgtgcgtttt gggtgag
//
LOCUS       DASTSF010000522         3821 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_766337,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000522 DASTSF010000000
VERSION     DASTSF010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3821
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_766337"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..707)
                     /locus_tag="VFU15_14925"
     CDS             complement(<1..707)
                     /locus_tag="VFU15_14925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEU4719134.1"
                     /translation="MNKVLLFNPRAAEHKPRIPNSVLQVAASIEGKYEWSIVDGNIEE
                     APVAKLFHYLRTGEYAYFGCTVMPGPQLRQAIIASSEIRKNFPSVKIAWGGYFPSNQP
                     EVCLRSGYVDAIINGPGDKSFPALLDAWEKNETVPGEIPNLIFLREGRMITTQKDAIY
                     EQDTLPDLPYEKLDAIYPLKRYLGKTYLGTRTIAYHSSFGCPFTCSFCAVVPVYNARW
                     KGKSAGKIYNDIKFLKE"
     gene            complement(730..2229)
                     /locus_tag="VFU15_14930"
     CDS             complement(730..2229)
                     /locus_tag="VFU15_14930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719135.1"
                     /translation="MKNVTRTTIILTALYSVLLFAGIVHHEMWLDETHHWLLARDSGS
                     LRELFHNSRYEGHPMLWDMLLFLIARFTQDPFAMQVMNFIVSVTGVFLFLRYFPFNTG
                     LKAVTVFSYFIFYEYGVISRNYAIAFLFLVATLKLFEDRRKHFLLFSVMLALLAQTHL
                     FASVIAGGLFAFAAWENYFRRSEGRFSNFYAGATIVALSFLLLVPEIIPPADHFLFRY
                     DPDPMISFKRFGKALSVLWKGLFPVPDFGSPHPWNSNWFISASKSVSVIPAALSWLVP
                     GMLLMKKRPVAVFYYSTAFVIVLFIYFSPLIVASRHCGFFFLLLIAGLWMMKTYKPGT
                     VYNRKVFRWMENCSEKYSPVILAVLFFCQLVSSVFLFYTDWKRPFSNGKYVAEWLKAD
                     AVKEPVLVSQMSAGPSICAYTGGTAFYPENNCVHSFCFWNTDPFIIPDDSLLVRAHRL
                     AANDPSRKALLVLNFRLDSVAMPPHVRFVRTFTGAMVRPEDYYIYEAEP"
     gene            complement(2234..>3821)
                     /locus_tag="VFU15_14935"
     CDS             complement(2234..>3821)
                     /locus_tag="VFU15_14935"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HEU4719136.1"
                     /translation="QKMPATEDKNRLKKNAALAALLLFAAVLFFSIRNYGQPLSDYGN
                     YYFGSFFFAHGVSTGKLYDPWEFNLLIDQSGAKHLYENYTPVPPLTLAAYLPFVLFTA
                     AVSKIIFMLLSGAFFSFSLYRFLLRHAVPFAFLAVAVLLFPLRANVEAGQSYFLVAGL
                     LLSGWMFYGDKKYLPAALLWALAIHLKVFPGIVFLFLLFEKDWKMIAYLFAAGVAFFL
                     AALPFTGFGVWENYFTEILPRLSVNEINNPFTFLSQSGTTFLRRLFVRDELLNPGAPY
                     NSPSLFYLLNSVYVLLLLLPVAMVSGDKKQNTFAKFSLWLFAGVLVSGYSTEYGLFLL
                     LFPLLSAGMRETKITGRWIAFLVVAAVAASFPVHRFLYETDPVRFSRLWMLLVFFVMM
                     VITLRPGLRFSRAMLFLLLIPVRYFPWHEAEDRSTYFLTGEIELLTGDYNWKNDTLVM
                     QVRTPEGIAQVENPVNETAEEDKLLAVKDGQIFDHGKQVTFSGDHKKKPMRTGDGYIV
                     YLSDKNRGVGFYALRKIKPE"
BASE COUNT         1048 a         1020 c          976 g          777 t
ORIGIN      
        1 ttctctttca ggaattttat gtcgttgtag attttcccgg ccgattttcc tttccaccgc
       61 gcattgtaaa cgggcaccac cgcgcagaac gagcaggtga acgggcagcc gaagctggaa
      121 tgatacgcga tcgtccgcgt cccgagatac gttttcccga ggtaacgttt cagcgggtag
      181 atcgcatcga gcttttcata gggaagatcg ggcaacgtgt cctgctcgta gatcgcatct
      241 ttctgcgtcg tgatcatccg gccttcgcgc aggaaaatga gattcggaat ttcaccgggg
      301 accgtctcat ttttttccca cgcatccagc aacgcaggga aacttttatc gcccgggcca
      361 ttgatgatcg catcgacata tccggaacgc aggcagactt ccggctggtt cgacgggaaa
      421 taaccgcccc aggcgatctt cacgctggga aaatttttcc tgatctcaga agaagcaatg
      481 atggcctgcc gcagctgcgg acccggcatc accgtgcagc cgaaataagc gtattctccc
      541 gtgcgcaggt aatggaacag tttcgcaacg ggcgcttcct cgatgttccc gtcgacgatg
      601 ctccattcgt atttcccttc gatggaagca gccacctgca ataccgaatt cggtatcctc
      661 ggcttgtgct cagcagcacg cgggttgaaa agaaggactt tgttcaaaat gaaatacggc
      721 tgaacgaatt tacggttccg cttcgtagat gtaataatct tccggtcgaa ccatcgcacc
      781 ggtaaaggtg cgaacgaaac ggacatgcgg gggcatcgcc accgaatcaa ggcggaaatt
      841 cagcacgagc agcgctttcc gcgacggatc attcgcagca agacgatgcg cacgcacgag
      901 cagcgagtcg tccgggatga tgaatggatc cgtattccag aagcagaagc tgtggacgca
      961 gttgttctcg gggtaaaagg cggttccgcc cgtgtaggcg caaatggatg gccccgcgct
     1021 catctggctc acgagcactg gttctttcac agcatccgcc ttcagccatt ccgccacata
     1081 ttttccattg gagaacggcc gtttccagtc ggtgtaaaag agaaaaacgg atgacaccag
     1141 ctggcagaaa aacaagaccg caaggatgac cgggctgtat ttttccgaac agttttccat
     1201 ccaccggaac actttcctgt tgtacactgt tcccggtttg tatgttttca tcatccacaa
     1261 accagcgatc agcaggagga agaaaaatcc gcaatgcctc gaagcaacga tcagcggtga
     1321 aaaatagatg aatagcacga tcacgaatgc cgtgctgtaa taaaacacag ccaccggcct
     1381 tttcttcatc agcagcattc cgggcaccag ccagctgagc gctgcgggaa tgacactgac
     1441 cgatttcgaa gctgaaatga accagttgga attccaggga tgcgggctcc cgaaatcggg
     1501 aaccgggaac aagcctttcc acagcacgga caacgctttt ccgaaacgtt tgaaggagat
     1561 catcgggtcc ggatcgtaac ggaaaagaaa atgatcagcc gggggaataa tttccggaac
     1621 aagcagcagg aaggacaacg cgacaatggt cgcacctgcg tagaaattac tgaaccggcc
     1681 ttcactgcgc cggaaataat tttcccaggc cgcgaaagca aacaatccgc ccgcgatgac
     1741 ggaggcgaaa aggtgcgtct gcgcaagcag cgcgagcatc acggaaaaga gaaggaaatg
     1801 cttccgccgg tcttcgaaaa gtttcagtgt cgccacaaga aaaagaaaag cgatcgcgta
     1861 gttcctgctg ataacgccgt attcatagaa aataaaatag ctgaacaccg tcacggcttt
     1921 caacccggta ttgaacggaa aataacgcag gaacaggaag acacccgtta cgctgacaat
     1981 gaaattcatc acctgcattg cgaacggatc ctgtgtgaaa cgtgcgatca aaaacagcag
     2041 catatcccac agcatggggt ggccttcgta gcgcgaatta tggaacaatt cccgcagcga
     2101 accgctgtcg cgcgcgagca gccagtgatg tgtttcatcc agccacatct catgatgaac
     2161 aatacccgcg aagagcagca cgctgtacag cgctgtcaga atgatcgtcg tccgcgttac
     2221 gtttttcatc cggttattcg ggctttattt ttctcaaggc gtaaaatccc acgccgcggt
     2281 ttttatccga caaatacacg atgtacccgt cgccggtccg catcggtttc ttcttgtggt
     2341 cgccggaaaa agtaacctgt ttcccgtgat cgaagatctg tccatcctta acagcaagca
     2401 atttatcctc ctccgccgtt tcgttcaccg ggttctccac ctgcgctatc ccttcaggcg
     2461 tacgcacctg catcacgagc gtatcgttct tccagttgta atcacctgtg agcagttcga
     2521 tctctccggt aagaaaatac gtgctccggt cttccgcctc atgccaggga aaataccgca
     2581 cgggaataag cagcaggaac aacatcgccc ggctgaaacg caaccccggc cgcagcgtga
     2641 tcaccatcat cacaaaaaag acaagcagca tccagagtct tgaaaaacga acaggatccg
     2701 tttcatataa aaaccggtgc acggggaacg aagcggccac cgcggcgaca accagaaatg
     2761 caatccacct gccggttatt tttgtttccc gcattcctgc ggaaagcagc gggaacagca
     2821 ggaggaaaag accgtactcc gtggaatacc cgctgacgag aacacctgcg aaaagccaga
     2881 gcgaaaattt ggcgaaggtg ttttgcttct tatccccgga aaccattgct accggcaaca
     2941 gcagcagcag cacataaacg gaattgagca ggtaaaaaag agacgggctg ttgtacggcg
     3001 cacccgggtt cagcagctcg tcgcgtacga aaagacgtcg caggaaagtt gttccgctct
     3061 gcgaaaggaa agtgaacggg ttgttgatct cgttgaccga aagccgggga agtatttctg
     3121 tgaaataatt ttcccacacg ccgaaaccgg tgaacggcaa tgccgcaaga aaaaaagcaa
     3181 cgcctgcggc aaaaagataa gcgatcattt tccagtcttt ttcaaaaagc aggaaaagaa
     3241 aaacgatccc gggaaaaact ttcaggtgta tcgccagcgc ccagagcagc gcagcaggca
     3301 gatatttttt gtcaccgtaa aacatccacc ctgaaagcag gagcccggcc acgagaaaat
     3361 acgactgccc ggcttccacg ttcgcgcgca gcgggaacag caacaccgcc acggcaagaa
     3421 aagcgaacgg gaccgcgtga cgcaacagga aacggtaaag ggaaaaagag aaaaaggctc
     3481 cgctcagcag catgaaaata atcttcgaaa ctgctgctgt aaagaggaca aaaggaagat
     3541 aagccgccag cgtcagcggc ggtacgggcg tgtagttttc gtacaggtgc tttgcgcccg
     3601 attgatcgat gagaagatta aactcccacg gatcatataa tttaccggtg gatacgccat
     3661 gcgcgaaaaa aaaacttccg aaataataat tcccgtagtc ggaaagcggc tgcccgtaat
     3721 ttcttatcga aaaaaacagc accgccgcga acagcagcag cgccgccagg gccgcatttt
     3781 ttttgagccg gtttttatct tcagtcgccg gcattttctg c
//
LOCUS       DASTSF010000523         5239 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_816777,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000523 DASTSF010000000
VERSION     DASTSF010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5239
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_816777"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2294
                     /locus_tag="VFU15_14940"
     CDS             <1..2294
                     /locus_tag="VFU15_14940"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HEU4719137.1"
                     /translation="STATSLCAGTYTCTIADAAVPTCTVTQTFNITQPTAINIVPSQT
                     DVTCNALCNGTASVAVTGGSGSYTYTWTPAPGAGQGTANATAMCAGSYTCAIADASSP
                     TCTITQTFNITEPAALTNTQSSTDVSCNGACDGTATVNVAGGTAPYSYSWAPNGATTP
                     GVTNLCAGTYTCTVTDANGCTTTATFTITEPTALTVAAAGFNVSCNGACDGQIVAIPN
                     GGTPNYNFSWTTGCSSASCNSVCAGSYTVTVTDLHGCTATASATVTEPAAIVTTTSEV
                     DAHCNMPDGSASANATGGTGVPTYQWQNGGPANANYTGIVPGTYTVVVTDQNNCHDTT
                     SVTVGNIPGATTSITSTTDVSCSGSCDGAIVCPGATGGTAPYTYNWAPAGGTNPSTSN
                     LCPGTYTLTLTDATGCVFTITTTITEPPALTVTATATPSVVCEGQNVQLNAGGSGGTG
                     PYQYGWNAPPMTGATQNITPAVSATYTAYITDAHFCTDSTTVSVTVNSIPSALFTGDS
                     LAGCAPLCVNFTDLSSQLAPATITQWSWDFGDGNTSTLQNPQHCYTVAGVYSVTLTVT
                     NNGGCTNTIVMSNYIDVYANPVADFSAGPQPTTVLDPMIYFTDLSSGASSWSWNFGDV
                     FSNGSTQQNPSFAYDSAACYGVTLTVMTSNGCVDTTTHFICIDPDVALYVPNAFTPNF
                     DGVNDIFRPQGIGIDPEHYQLWIFDRWGNMIFTTTDWNKGWDGRVQGHEDLCQEDVYV
                     WKIKALDVNGKKHNVIGSVSLIR"
     gene            2368..2877
                     /locus_tag="VFU15_14945"
     CDS             2368..2877
                     /locus_tag="VFU15_14945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009412424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEU4719138.1"
                     /translation="MRIALLSDTHGYIDDRIISYCRSCDEIWHAGDVGNADVIHTLEK
                     TKPVRGVSGNIDGANVQSIFPKQNRFRCEDVDVLMIHIGGRPGNYFPEARKIIEKDPP
                     KLFICGHSHILLVQYDKQYNMLHMNPGAAGMSGFHKMRTMLRFTIEGKNIKDAEVVEL
                     GRRGVSARD"
     gene            3083..>5239
                     /locus_tag="VFU15_14950"
     CDS             3083..>5239
                     /locus_tag="VFU15_14950"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014681209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HEU4719139.1"
                     /translation="MTFDLDMIRKVYGQLPSRVEAARKLAGRPLSLTEKILYAHLCEA
                     LPAKAYERGKDYVDFNPDRVAMQDATAQMALLQFMQAGRKKAAVPSTVHCDHLIVAKA
                     GAGDDLVLANKDNKEVYDFLSSVSNKYGIGFWKPGAGIIHQVVLENYAFPGGMMIGTD
                     SHTVNAGGLGMIAIGVGGADACDVMAGLPWELKWPKLIGVKLNGKLSGWTSPKDVILK
                     VAGILTVKGGTGAIVEYFGEGAIAMSCTGKGTICNMGAEIGATTSTFGYDDSMERYLR
                     ATGRSEVADLANGIREHLTADAEVYANPEKYFDQVIEINLSELEPHLNGPFTPDLATP
                     VSKMKEAAEKNGWPVKVEVGLIGSCTNSSYEDISRAASLAEQAAAKHLKPKAVFTITP
                     GSEQVRFTIERDGLIKTFDKIGAKVFANACGPCIGMWSRTGSDRKEKNTIVHSFNRNF
                     QSRNDGNPNTHAFVASPELVTALAIAGDLTFNPLTDFLVNENGEKVKLEPPTGDELPK
                     KGFGVDDAGYQAPAADGSGVEVKVAPDSKRLQLLAPFAAWEGTDLKGLRLLIKAKGKC
                     TTDHISMAGPWLKYRGHLDNISNNLLIGATNFFNEKQNSVKNQLDGSYDEVPKVQRAY
                     KAAHIGSIVVGDENYGEGSSREHAAMEPRHLGVRAILVKSFARIHETNLKKQGMLALT
                     FANKDDYSKIKEDDTFDINGLTTFAPGVPLTIAIHHA"
BASE COUNT         1278 a         1450 c         1456 g         1055 t
ORIGIN      
        1 cttccactgc aacttcgctt tgcgcaggaa cctatacctg tacgatcgct gatgctgccg
       61 tacccacatg taccgtaacg caaaccttca acattacaca gcccacggcg atcaatattg
      121 ttccgtcgca aacggatgtc acctgcaacg cgctgtgcaa tgggacagct tctgtcgccg
      181 taaccggcgg atcgggttca tacacgtata catggacgcc cgcacccggg gcaggacagg
      241 gaacagcgaa tgcaacagca atgtgtgcgg gttcatacac ctgcgcaatt gcagacgctt
      301 cttcgccgac ctgtacaatt acgcagacat tcaatattac agaacccgcg gcgctgacga
      361 atacgcaatc atcgacggat gtatcctgca acggcgcgtg tgacggtact gcaaccgtta
      421 acgttgccgg cggcacggct ccgtattcat acagctgggc cccgaacggc gcgacgacac
      481 cgggcgtaac gaacctttgt gcgggaactt atacctgtac ggtgactgat gccaacggat
      541 gcacgacaac cgccacattt acgatcaccg agccgaccgc actgacggtg gctgcggcgg
      601 gattcaacgt aagctgcaac ggcgcatgcg acgggcagat cgtcgcgatc ccgaatggcg
      661 gaacaccgaa ttataatttc tcgtggacca caggatgttc ttccgcaagc tgcaacagcg
      721 tttgcgcagg ttcctacaca gtgacggtca ccgaccttca cgggtgtaca gcaacagcat
      781 ccgcaacggt taccgagccg gcggcaatcg tgactacgac ttcagaagtc gatgcacatt
      841 gcaacatgcc tgacggttct gcttctgcga atgcgacagg cggaacgggt gttccgactt
      901 atcaatggca gaacggcgga ccggcgaatg caaactatac aggcatcgtt ccgggaacgt
      961 ataccgttgt tgttaccgac cagaacaatt gccacgacac aacttcggta accgtaggca
     1021 atatccccgg cgcaacaaca agcattacct cgacaaccga tgtcagctgt agcggatcgt
     1081 gcgacggcgc gatcgtttgc cccggcgcaa cgggaggaac ggcgccctat acttacaact
     1141 gggcacctgc cggaggaacg aacccgagca cgagcaacct gtgtccggga acatacaccc
     1201 tgacattgac cgacgcaacc ggatgtgtat tcacgatcac gaccacgatc actgaaccgc
     1261 ccgcgctgac ggtcactgcg acggccacgc cgagcgtcgt ctgcgaagga cagaacgtgc
     1321 agctcaatgc cggcggcagc ggcggaacgg gtccgtacca gtatggatgg aacgcaccgc
     1381 caatgacggg cgcgacgcaa aacattacgc ccgctgtttc cgcaacctat accgcataca
     1441 taacggatgc gcatttctgt accgacagta caaccgtttc cgttactgta aacagcattc
     1501 cgtcggccct gtttaccggc gattcactgg ccggatgcgc tcctctttgc gtgaacttca
     1561 ccgacctttc ctcgcagctg gcgcccgcga ccatcacgca gtggagctgg gatttcggtg
     1621 acggaaatac gtcgacattg cagaacccgc agcattgcta tacggtggcc ggagtttatt
     1681 ccgttacact taccgtgacc aacaacggag gctgcacgaa cacgatcgtc atgtcgaatt
     1741 acattgacgt ttacgcaaac ccggtagccg atttctctgc aggtccgcaa ccgacgacag
     1801 tgctcgatcc gatgatctac tttacggatc tttcctccgg cgcttcttcg tggagctgga
     1861 acttcggcga tgttttcagt aacggatcga cacagcagaa cccgtctttc gcgtatgact
     1921 ccgcagcatg ctacggcgta acgctgacgg tcatgacgtc gaacggctgc gttgatacga
     1981 cgacgcattt catctgcatc gatccggatg tagcgctcta tgttcccaat gcctttacac
     2041 cgaatttcga cggcgtgaac gacatcttcc ggccgcaggg aatcggcatc gatcctgaac
     2101 attaccagct ctggatcttc gaccgctggg gcaacatgat cttcaccacg acggactgga
     2161 acaaaggatg ggacggaaga gtgcaggggc atgaagatct ttgccaggaa gatgtttacg
     2221 tctggaagat caaagcgctc gatgtcaatg gcaagaagca caatgtgatc ggcagcgttt
     2281 cgctgatccg ctgatcgaaa gaaaaaacac agggccactc ttaacgggtg gcctttttta
     2341 ttttgtggaa aaccaattca tttgcagatg cggatcgcgc ttctttccga cactcacggc
     2401 tacatcgatg acaggataat ttcgtactgc cggtcctgcg atgaaatatg gcatgcggga
     2461 gatgtgggaa atgcggatgt catccacacg ctcgaaaaaa caaaacctgt gcgcggtgtt
     2521 tccggcaaca tcgacggcgc gaatgtgcag agcatttttc cgaagcagaa ccgtttccgc
     2581 tgcgaagacg tcgacgtgct catgatccac atcggcgggc gaccggggaa ttattttccc
     2641 gaagcgagga agatcatcga aaaagatccg ccgaaacttt ttatctgcgg acattcccac
     2701 attctccttg tgcagtacga caagcaatac aacatgctgc acatgaatcc cggcgctgcg
     2761 gggatgagcg ggttccataa aatgcggacg atgctccgct ttaccatcga agggaaaaat
     2821 atcaaggacg cggaggttgt cgagctggga cggagaggcg tttctgcacg cgattaagtc
     2881 tggccgggcc gccaggattt tcaccgggaa gtttcgcgcc tgcgcgactg attttccggc
     2941 gctaagctac ttaggcgcaa aaaaatcaag gttcactccg gaaatgagct gctatcaggt
     3001 ctgaaatcgt cccgtttttc gcgtacgaat tcgtaaattt gtatcccttt tcaggcactt
     3061 aacactcaaa ataattctct ctatgacctt cgaccttgac atgatcagaa aagtttacgg
     3121 acagcttcct tcccgtgttg aagctgcacg taagctggcg ggccgcccgc tttccctcac
     3181 tgaaaaaatc ctttacgcgc atctctgcga agcgcttccc gcgaaagcgt acgagcgagg
     3241 aaaagattac gtcgatttca atcctgaccg cgtggcgatg caggatgcaa ctgcacagat
     3301 ggcgttgctc cagttcatgc aggcagggcg gaaaaaagcg gctgtccctt ccactgtgca
     3361 ctgcgatcac ctgatcgtgg caaaggcagg agccggtgat gatcttgtgc tggcgaacaa
     3421 agacaacaag gaagtgtatg attttctctc ttccgtttcc aataaatacg gcatcggctt
     3481 ctggaaaccg ggcgccggca tcatccacca ggtggtgctc gagaattacg cattccccgg
     3541 cgggatgatg atcggaacgg attcgcatac ggtgaacgcc ggcggactcg gcatgatcgc
     3601 gatcggtgtg ggtggagcgg atgcgtgcga cgtgatggcg ggacttccgt gggagctcaa
     3661 atggccgaag ctgatcggtg tgaagctgaa cgggaagctg agcgggtgga catcaccgaa
     3721 agatgtcatc ctgaaagtgg cgggcattct taccgttaaa ggcggaacgg gcgcgatcgt
     3781 ggaatatttc ggcgaaggtg cgatcgccat gagctgcacg ggcaaaggaa cgatctgcaa
     3841 catgggcgct gagatcggtg cgacgacgtc cacgttcggt tatgacgatt cgatggagcg
     3901 ttatctccgt gcgaccgggc gcagcgaagt ggctgatctt gccaatggca tccgcgaaca
     3961 tcttactgcc gacgcggaag tgtacgcgaa cccggaaaaa tatttcgacc aggtgatcga
     4021 gatcaacctg tcggagctgg agccgcacct caacggcccg ttcacaccgg accttgcgac
     4081 gcccgtcagc aaaatgaaag aagctgccga gaagaacggc tggccggtga aagtggaagt
     4141 ggggctgatc ggttcgtgca cgaattcctc gtacgaagat atttcacgcg ctgcatcgct
     4201 tgcagaacag gcggccgcga agcacctgaa accgaaagcg gtgttcacca tcacgccggg
     4261 ctccgaacaa gtgcgtttta ctattgaacg cgacggcctg atcaaaacat tcgacaagat
     4321 cggcgcgaaa gtttttgcga atgcgtgcgg accctgcatc gggatgtgga gcaggaccgg
     4381 ttccgaccgc aaggaaaaaa acaccatcgt tcattcgttc aaccgcaatt tccagagccg
     4441 caacgacggc aacccgaaca cgcatgcgtt cgtcgcttca ccggaactgg tgacagcgct
     4501 ggcgatcgcc ggcgatctta cgttcaaccc gctgacggat ttcctggtca acgagaacgg
     4561 ggaaaaagtg aagctcgaac ccccgacagg agacgagctt ccgaaaaaag gtttcggcgt
     4621 ggacgacgcc ggctaccagg cgcccgcagc agacggaagc ggtgtggaag tgaaggtcgc
     4681 tccggacagc aagcgtttac agttgctcgc gccgttcgcg gcgtgggaag gaaccgatct
     4741 caaaggactt cgtctcctga tcaaggcgaa aggaaaatgt accaccgatc atatttccat
     4801 ggcaggtccg tggctgaaat accgcggtca cctcgacaat atttccaaca acctgctcat
     4861 cggcgcgacg aatttcttca atgagaaaca gaacagcgtg aagaaccagc tcgacggttc
     4921 gtacgatgaa gttccgaaag tgcagcgcgc gtacaaggcg gcgcatatcg ggtcgatcgt
     4981 ggtgggtgat gagaattacg gggagggctc gtcgcgtgag catgcagcga tggaaccgcg
     5041 tcacctcggc gtgcgtgcga tcctcgtgaa atcatttgcg cgcattcacg aaaccaacct
     5101 caagaaacag ggaatgcttg cgctcacttt cgcgaacaag gacgattaca gcaagatcaa
     5161 agaggacgac acgttcgaca tcaacgggct gacaacgttc gcacccggtg ttccgctgac
     5221 gatcgctatt catcacgcg
//
LOCUS       DASTSF010000524         6557 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_294424,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000524 DASTSF010000000
VERSION     DASTSF010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 6557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6557
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_294424"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..968
                     /locus_tag="VFU15_14955"
     CDS             <1..968
                     /locus_tag="VFU15_14955"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU4719140.1"
                     /translation="AHKTDATIFAKLDMQVSEATSIFVDLQYRNVGYKFTGVDSAGNP
                     LPQSVQLNFFNPKAGFAWKISAVNTAYISFSTGNKEPNRDDYTQSTALSRPKPETMYD
                     IETGWKYSSNHLQLTANYFYMYYINQLVLTGKVNDVGAYTRENVSQSYRTGIELCGDW
                     KISDRFAFSANATFSQNKILNYNEYIDNYDSARQDVVFHASPDIAFSPRVTAFAGLTF
                     TGKKGLEISLMEKYVSKQYLDNTSDEAAKLDPYAVTNMTVSYTFHPRGVKELRLGVQL
                     NNLTNAMYSSNGYTYGYVYGGTYNRFNYYYPQAGFNTMGMISLKF"
     gene            complement(1013..1666)
                     /locus_tag="VFU15_14960"
     CDS             complement(1013..1666)
                     /locus_tag="VFU15_14960"
                     /inference="COORDINATES: protein motif:HMM:NF025224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HEU4719141.1"
                     /translation="MKNNPRRFPPFKIPLALIFCVAFSLRMHAQQPAAPALPLYDSLT
                     LDTMTAFTDLNEAMKNPDKVVKLVLRKQHLKSFPKEIWNFPNLQYLDLSKNQISDFPD
                     SLGKLKNLQVLHLSRNKIESLPREIGDLSNLKILDINQNELIALPPQIGKLTNLEYLD
                     LWSNNLSIFPDELKNISGNLKVLDLRVILINYETQDRIKRLLPNTTIYFSPPCKCNE"
     gene            complement(1656..2588)
                     /locus_tag="VFU15_14965"
     CDS             complement(1656..2588)
                     /locus_tag="VFU15_14965"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019596934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEU4719142.1"
                     /translation="MKVYNDISEFGHVAVPVLTTGTFDGVHRGHMQIISRLKESSRSM
                     NGETVIFTFFPHPRMVLFPDHRQVLLNTLREKTAKLEEAGIDHLIIQPFTREFSMLSS
                     KDFIEKILVEKIHTRKLVIGYDHHFGRNREGSFEHLRNSGPVYGFEVEEIPAKLIDNV
                     SVSSTRIRHSLEKGDVESANANLGYRYPLTGMVVKGRQLGRTIGFPTANILVEDPYKL
                     VPADGVYAVMVKRGNETHKGMLNIGMRPTVDGRERTIEVNILDFDADLYGETLTLEFV
                     KRLRDEIKFSGLEELKKQLFADRTHAMGILENEK"
     gene            complement(2677..4866)
                     /locus_tag="VFU15_14970"
     CDS             complement(2677..4866)
                     /locus_tag="VFU15_14970"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor J domain-containing protein"
                     /protein_id="HEU4719143.1"
                     /translation="MKKFLLSFSLLAGVCAISHAQILFSEDFDGLPGPTAGGAGTYVF
                     PNGWFLRNVDNGTPATSVSYVNDAWERREDFGFNVADSAAFSTSWYSPAGTADDWMWT
                     PLIGPLPANSVLTWSARAYDPSYSDGYEVRIMTAAQGPPTGGTGVIGNQITNSTVLFS
                     VAAENTTWTQHSVNLNAYAGQSVYISWRNHSTDKFLLVVDNVVVQVQVNIDAQMLFAD
                     TATQYTQIPQPQGAPLNFNGTIRNNGINTLSNVSAQVNVFNGATNVYSASSTPTASLA
                     STASASWTVAPFTPTAVGNYTVQFIANQTSGTDQVPANDTLYQYFTVTDTTYARDDGM
                     VTGSLGIGAGNGGYLGQDYDLVTSDTLTSVSMYVTRGYTGKMAGIAIWNMASGTPTTI
                     AAVTDTILYPDDSARFYTIPIYGGGIVLSPGKYAVTAIEFDSTLALGQTNTVFTTNTT
                     WVNWPTSPLGGWGNNEDFGSSFAKSYVIRPNFGNICADNMATATATTATCLTCADGSA
                     SVTATGTNGPLTYSWSPSGGTGATASNIPTGIYTVTVTDGFGCVSTAMVSVAYDTCSS
                     FNAILDSTAASCGTCADGIASATPVNNMGPVTYLWSTGDTTSTVTGLLPGTYTVTITD
                     SLGCTYTDSLHIGFSTVITDLASQGKISVYPNPNDGQFVLNIDLDHTADVKVVVVDML
                     GQEIMNNEYSGVTSRRIDINLGDDAVGLYTIRVLTKDATWVVPVNVR"
     gene            5259..>6557
                     /gene="atpD"
                     /locus_tag="VFU15_14975"
     CDS             5259..>6557
                     /gene="atpD"
                     /locus_tag="VFU15_14975"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020604451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEU4719144.1"
                     /translation="MQTGKIAQVIGPVIDVSFSDQGGSLPNILDALYVTRANGQKVIL
                     ECQQHIGEDTVRTVAMDSTDGLSRGMDVVATGAPIKMPIGEQIRGRLFNVVGEAIDGI
                     KPISNEGGYAIHRQPPKFEELSTSAEVLFTGIKVIDLIEPYSKGGKIGLFGGAGVGKT
                     VLIMELINNIAKGYSGFSVFAGVGERTREGNDLLREMIESGVIKYGEEFTHSMEKGGW
                     DISKVNMDELAKSQATLVFGQMNEPPGARARVALSGLTLAEYYRDGDEKSGGRDILFF
                     IDNIFRFTQAGSEVSALLGRMPSAVGYQPTLATEMGAMQERITSTKRGSITSVQAVYV
                     PADDLTDPAPATTFAHLDATTVLSRKIAELGIYPAVDPLDSTSRILSPAVVGEEHYGT
                     AQRVKNILQRYKELQDIIAILGLDELSEDDRVTVARARKIE"
BASE COUNT         1495 a         1667 c         1852 g         1543 t
ORIGIN      
        1 cggcgcataa gaccgatgcg accatctttg ctaagctgga tatgcaggtt tcagaggcga
       61 cgagcatttt tgtagacctg caatacagga acgtgggtta caaattcacg ggtgttgaca
      121 gtgcaggaaa cccgcttccg caaagcgtgc agctgaattt tttcaatccg aaagccggct
      181 tcgcctggaa gatcagtgcc gtgaacaccg cgtatatttc cttcagcacc gggaacaaag
      241 agcccaaccg cgacgattac acccaatcga ccgcattaag ccggccgaag cccgaaacga
      301 tgtacgacat tgaaaccggg tggaaatatt cgtccaacca tttgcagctg acggcgaatt
      361 atttttacat gtactacatc aaccagcttg tgctgaccgg gaaagtgaat gacgtcggcg
      421 cgtacacgag ggaaaacgtt tcgcagagct accgcacggg aatcgagctg tgcggcgact
      481 ggaagatctc ggatcgtttt gcattctcgg cgaacgcgac gttcagccag aacaagatcc
      541 tcaactacaa cgaatacatc gacaattacg acagcgcgcg ccaggatgtt gtttttcatg
      601 cctcgccgga catcgccttc tcgccgcggg tgaccgcgtt cgcaggactt acatttaccg
      661 gaaagaaagg actggaaatt tcgctgatgg aaaaatacgt cagcaagcaa tacctggata
      721 atacgtcgga cgaggcggca aagctcgatc cgtatgccgt gacgaacatg accgtttcct
      781 ataccttcca tccgcgtggc gtgaaagagc tgcgtctcgg tgttcagctg aacaacctca
      841 cgaatgcaat gtattcttcg aatggttata cctacggtta cgtttacggg ggcacgtaca
      901 accggttcaa ttattattac ccgcaggccg gtttcaatac gatgggaatg atctcgctga
      961 aattctgaaa cggagttcag cgcaaaggaa aagtccgccg cggcggactt ttttattcat
     1021 tgcatttgca gggcggagag aaatagatcg tggtgttcgg gagcaagcgc ttgatgcggt
     1081 cctgcgtctc gtaattgatg agaatgacgc gcaggtcgag cactttcagg ttcccgctga
     1141 tgttcttcag ctcatcgggg aaaatggaaa ggttgttgct ccagagatcg aggtattcca
     1201 ggttggtcag cttgccgatc tgcggcggca gggcgatcag ctcgttctgg ttgatgtcga
     1261 ggattttcag gttggagaga tcgccgatct cgcgcgggag cgattcgatc ttgttgcgcg
     1321 aaagatgcag cacctggagg tttttcaatt tgcccagcga atcgggaaaa tccgagatct
     1381 ggtttttgct caggtcgagg tactgcaggt tcgggaaatt ccagatctct ttcggaaagc
     1441 ttttcaggtg ctgtttgcgc agcacgagct tcaccacttt gtccgggttc ttcatcgcct
     1501 cgttcaggtc ggtgaaggcg gtcatcgtgt cgagcgtcag tgaatcgtaa agcgggagcg
     1561 caggagcggc gggttgctgt gcgtgcatgc gcagcgagaa ggccacgcag aaaatcagcg
     1621 caagcgggat tttaaacggg ggaaaacgtc ggggattatt tttcattttc aagaatgccc
     1681 atggcatgcg ttctgtcagc aaacaattgt tttttcaatt cttcgagccc gctgaatttg
     1741 atctcgtccc tgaggcgttt tacgaactcc aaggtaagcg tttccccgta aaggtctgcg
     1801 tcgaaatcaa gaatgttgac ttcaattgtg cgttcgcgtc cgtcgactgt cgggcgcata
     1861 ccgatgttca gcatgccttt gtgggtttca tttccgcgct tcaccatcac tgcataaacg
     1921 ccgtctgccg gcaccagttt gtacggatct tccacgagaa tgttcgccgt ggggaacccg
     1981 atcgtgcggc cgagctgtct tccttttacc accattcccg tcagcgggta gcggtagccg
     2041 aggtttgcat ttgcgctttc cacgtcgcct ttttcaagtg aatggcgtat cctcgtcgag
     2101 ctgacgctta cgttgtcgat cagtttggca gggatctcct ccacttcgaa tccataaacc
     2161 gggccggaat tgcggaggtg ctcgaagctg ccttcccggt tacgcccgaa atgatggtcg
     2221 tacccgatca cgagctttcg cgtatggatc ttttccacga ggatcttttc aatgaaatcc
     2281 ttcgaggaaa gcatggagaa ttcgcgcgtg aacggctgga tgatcaggtg atcgataccg
     2341 gcttcttcca gctttgccgt tttttcacgc agcgtgttga gcaggacctg ccggtgatcg
     2401 ggaaacagca ccatgcgcgg atgcggaaaa aaagtgaaga tgacggtttc cccgttcatc
     2461 gaccgggaag attccttcag ccgtgaaatg atctgcatgt gtccgcggtg gacaccatcg
     2521 aacgtacccg tggtcagcac cggaaccgca acatggccga attcgctgat atcgttgtaa
     2581 actttcacgc ctgcaaaggt aaataagaac gaagcctttg ttttctgaga aaaagaaaac
     2641 cccgccgggg cggggcgttc tttccggaaa aaaaatttac ctgacgttga ccggtacgac
     2701 ccaggttgcg tctttcgtca gcacgcgaat cgtgtaaaga ccgacagcgt catcgccgag
     2761 attaatatcg atcctgcgcg aagtcacgcc tgaatattca ttgttcatga tctcctggcc
     2821 cagcatatcg acgacaacga cctttacgtc agccgtatgg tcgagatcga tgttgaggac
     2881 gaattgtccg tcattcgggt tcggataaac ggagatcttg ccctgcgaag ccaggtcggt
     2941 gataaccgtc gagaagccga tgtgcagcga atcggtatac gtacagccga gcgagtcggt
     3001 gatcgttacc gtatacgttc cgggaagcag gcccgtaaca gtgctcgtcg tatctccggt
     3061 gctccagaga taagtgacgg gtcccatgtt attgaccggt gttgccgaag cgattccgtc
     3121 cgcgcaggtg ccgcacgatg ccgcagtgga atcaaggata gcgttgaaag aagagcaggt
     3181 gtcgtatgca accgaaacca ttgccgtgct tacacagccg aaaccatccg ttaccgtaac
     3241 ggtatagatt ccggtgggaa tattcgatgc ggtagcaccg gttccgccgg aaggactcca
     3301 ggaataggtg agcggtccgt tcgtcccggt cgcggtgacg cttgcgcttc cgtctgcgca
     3361 ggtcaggcag gtggcggttg tggctgtggc cgtcgccatg ttgtcagcgc aaatgttccc
     3421 gaagttgggg cgaatgacat acgacttcgc gaacgacgat ccaaaatcct cgttattgcc
     3481 ccagccgccg agcgggcttg tgggccagtt cacccacgtc gtattcgtgg tgaacaccgt
     3541 attggtttgc ccgagcgcca gcgtgctgtc gaactcgatg gcggtgactg cgtatttgcc
     3601 cggtgaaaga acaatgcctc cgccgtagat cggtatcgtg tagaagcggg ccgaatcatc
     3661 agggtaaagg atcgtatccg taaccgcagc gatcgtcgtg ggtgttcccg acgccatgtt
     3721 ccagatggcg atgccggcca tttttcctgt atacccgcgt gtgacgtaca tgctcacgga
     3781 tgtcagcgta tcgctggtca cgagatcgta atcctgcccg aggtaaccgc cgttccccgc
     3841 gccgatgcca agacttcctg tcaccattcc gtcgtcccgc gcataagttg tgtcggtaac
     3901 ggtgaaatac tggtacaatg tatcattcgc gggaacctgg tcggttccgc tggtttgatt
     3961 ggcgatgaac tgcacggtgt aattgccgac agccgtggga gtgaaaggag ccacggtcca
     4021 gcttgccgat gcggttgacg caagcgaagc tgtcggtgtg gagctggccg aatacacgtt
     4081 cgtcgcgccg ttgaaaacgt tcacctgggc gcttacgttt gaaagtgtat tgatcccgtt
     4141 gttgcggatg gtcccgttga aattcagcgg tgcgccctgc ggctgcggga tctgcgtata
     4201 ctgcgtagct gtatccgcaa aaagcatttg cgcatcgata ttcacctgca cctggacaac
     4261 gacgttatcc acgacgagaa ggaatttgtc ggtggagtga ttacgccagg aaatgtaaac
     4321 cgattgacct gcatacgcgt tgaggttgac ggagtgctgc gtccacgtag tgttttctgc
     4381 ggccacggaa aaaaggaccg tggaattcgt gatctggttt ccgatcaccc cggttccgcc
     4441 ggtgggcgga ccttgtgcgg ccgtcattat cctgacctca taaccgtcag aataagaggg
     4501 atcataagcg cgcgcgctcc aggtcaggac ggaattggcc ggaagcggac cgatgagcgg
     4561 tgtccacatc cagtcgtcgg ctgtgccggc cggagaatac caggaagtgc tgaacgcggc
     4621 tgaatcggct acgttaaaac cgaaatcttc gcggcgttcc cacgcatcgt tgacataaga
     4681 gacacttgtg gccggtgttc cgttatccac attgcggaga aaccatccgt tgggaaaaac
     4741 ataggttccc gcgccacctg cagtgggtcc cgggagcccg tcgaaatctt cactgaaaag
     4801 aatctgcgca tgtgagatgg cgcatacgcc ggcaagaaga gaaaatgaga gtagaaattt
     4861 tttcatttta tggaattgaa ttttccaaaa gtaagtttct ttttgcaaac agaggggaac
     4921 attccgtacg gaatacatgg tctttacgaa tagtttttac tgcgttcaca gtttctgttt
     4981 ccggaaggaa aacagttccc ggcatgcagg caaaaggaag aatgttgtgc ggaaaaacat
     5041 acggttgcgg cggcaggcaa taggaaaaaa tgcaggcagt ggttgatccg ccctgttttt
     5101 ttgcgctgat tttcgtcaga ttttttgcgc aggctgtggg tggtttggcg gattctgtcg
     5161 gaacgcgggc aagttcttct cattttatca acatcacccg atccaaaccg gaataaactt
     5221 tctatttttg cggcggattt ttagaaccta tcctatccat gcaaacagga aaaattgccc
     5281 aggttatcgg tcccgtgatc gacgtaagtt tcagcgacca gggaggatca ctcccgaata
     5341 ttctcgacgc gctttatgta acgcgcgcca acggacagaa agtaattctc gagtgccagc
     5401 agcacatcgg tgaggatacg gtaaggaccg ttgcgatgga ttccaccgac ggactttccc
     5461 gcggaatgga tgttgtcgcc acgggtgcac cgatcaaaat gccgatcggc gagcagatcc
     5521 gcggaaggct gttcaacgtg gtcggcgaag cgatcgacgg tattaagccg atcagcaacg
     5581 aaggcgggta cgccattcac cgccagcccc cgaaattcga agagctttcc acatctgcgg
     5641 aagtgctttt cactggcatc aaagtcatcg acctaattga gccgtattca aaaggcggaa
     5701 agatcggtct gttcggcggt gccggtgtgg gaaagacagt tcttatcatg gaactcatca
     5761 acaacatcgc gaaaggctat tcgggatttt ccgtgttcgc cggcgtcggt gaacgtacgc
     5821 gcgaagggaa tgaccttctc cgcgagatga tcgaatcagg cgttatcaaa tacggtgaag
     5881 agttcacgca ttcgatggaa aaaggtggct gggacatttc caaagtaaat atggatgagc
     5941 tggcgaaatc acaggccacg ctggtgttcg ggcagatgaa cgaacctccc ggagcccgtg
     6001 cgcgtgtggc gctttccggt cttacgctcg cggaatacta ccgcgacggc gatgaaaaat
     6061 caggcggacg cgacatcctt ttctttatcg acaatatttt ccgttttacg caggcaggtt
     6121 ccgaagtatc ggcgctgctc ggacgtatgc cttccgccgt gggttaccag cctacgctcg
     6181 ccaccgaaat gggcgcgatg caggaacgca ttacttccac caagcgcggt tctatcactt
     6241 ccgtccaggc cgtttacgtt cctgcggatg accttaccga ccctgcaccg gcaacgacgt
     6301 tcgctcacct tgatgcgacc acggtattga gccgtaagat cgccgagctt ggtatttacc
     6361 cggccgttga tccgctggat tccacatcgc gcattctttc ccccgctgtc gtcggcgaag
     6421 agcattacgg cacggcgcag cgcgtgaaga atattctcca gcgctacaaa gagctgcagg
     6481 acatcatcgc gattctgggt cttgacgagc tttctgaaga tgaccgtgtg acggtggctc
     6541 gggctcggaa gatcgag
//
LOCUS       DASTSF010000525         4279 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_766675,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000525 DASTSF010000000
VERSION     DASTSF010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4279
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_766675"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(186..914)
                     /locus_tag="VFU15_14980"
     CDS             complement(186..914)
                     /locus_tag="VFU15_14980"
                     /inference="COORDINATES: protein motif:HMM:NF013357.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH25 family lysozyme"
                     /protein_id="HEU4719145.1"
                     /translation="MKRKIIIAALVVLLATGITLLWLFRFHIRFNYPSEKEFPVRGID
                     ISHHNGVIDWQKLRAENFSFVFMKATEGGDYTDPSFSASCAEAQENNFIVGAYHFYSF
                     CRNGNEQAAHFIEVVPHLPGKLPPVLDLEYSGNCKTAKPKTQIIQEIGDCIDSLKNYY
                     GVAPVLYCTSDFYTDYSLSRFSSCPVWIRNVYSRPEELNGRKWTFWQFASNAHAEGIS
                     ERVDLDVFNGDTAAFRKFTETNAR"
     gene            complement(915..1760)
                     /locus_tag="VFU15_14985"
     CDS             complement(915..1760)
                     /locus_tag="VFU15_14985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719146.1"
                     /translation="MKRIPCFLFFLLVFASCDEKSSPPGKSVDSVMRRFSFIRMNTSE
                     PPRQKTVPDSTFLRIMRQDAFFHRHDTIVKQEFRAFACGSDSFWVIRTQKGDSVYTRV
                     YKKNKRYRLVISDEEGWDWSPWDTVLDADFDGYADYLLGIYSPCGCCPRENYHVYFYD
                     PGMHSLRDNDVYVMNPYFDSASKSVYCMDYGFPYPPQPAGLTKYRWSGDSLVETEGVD
                     YDWPDTVLSLTNLAKTRRGSSPHDSLLRDLPAPYKKIPQVEFWFYTPADSLRMVRYFS
                     SNAKR"
     gene            complement(1757..2200)
                     /locus_tag="VFU15_14990"
     CDS             complement(1757..2200)
                     /locus_tag="VFU15_14990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEU4719147.1"
                     /translation="MAGLKTKKTTASVEDFLDAIKDEERRSDCHAIVKLMKKVTKAEP
                     KMWGPAIVGFGDYRYVYDSGREGDWFKMGFSPRKQNLTLYSMGGLARFTSLLKKLGKH
                     KTGKGCLYIDRLADIDKKVLEKLLSTSLAEMKKHSEAQKAKLKKK"
     gene            complement(2273..2893)
                     /locus_tag="VFU15_14995"
     CDS             complement(2273..2893)
                     /locus_tag="VFU15_14995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719148.1"
                     /translation="MKTFAFPLLFLLLLVSACDSEPEKQPQQKPPAVALNDSATKISA
                     RGDSLSYAYADMLYDEAVSLDSNYYIAYWNKLACETELHFYAKALATARELIRLRPGN
                     PDHRITAGMLAQHTGDTALAEKYYDEGIALFRSITDTSPVTSRKYYSMMTMYGLSLIV
                     AYHTSEGDSVLAKVIANRQDTLSRNLARKFYGKSRDELIAAMEGKQ"
     gene            complement(2948..>4279)
                     /gene="lnt"
                     /locus_tag="VFU15_15000"
     CDS             complement(2948..>4279)
                     /gene="lnt"
                     /locus_tag="VFU15_15000"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HEU4719149.1"
                     /translation="IVFFAFHKVKRMLPEKYGTFVLIPFWIIFEHFHLDWDLSWPWLT
                     LGNGLVNNYTWIQWYEYTGVFGGSFWILLINVLLFEFLRRRNLENEKKARKILVAVTL
                     SVLLLPVLVSYIIYFRFDVNANTTYPLDVVVVQPNIDPYQKFTSDFRDETEKMLSLAA
                     AKTDSSVDYVVMPETALVESIWENDIQSSWSIHRLRKFRERYPKLNIVTGATTGYYYK
                     AGEKLSSTARKFSDGDEYYDNYNTALEIDSAQAIQLYHKSKLVPGVEKMPFPQYLGFL
                     DKYAIDLGGTVGSLGMQDERTVFRSPNGPGKVAPVICYESIYGDYVSQYIRNGADYIF
                     IITNDGWWGNTPGYKQHLLYGRLRAIETRKSIARAANTGISCFIDQRGDFFQRQPWWT
                     ASAIRQKIVSTAGMTFYTEHGDYIVTIMDIFALLAITGGLVRMIMRKRAVK"
BASE COUNT          971 a         1057 c         1211 g         1040 t
ORIGIN      
        1 gcagaaaaga aaaataggca gccagaaaag gaattcgcgg ttgacaggga tcacatagac
       61 cgtcgccacg gccatggccg gccctaaaag ggaaaggaga aagataagcc gccagctcat
      121 gggtgaatgg ttttggcagg aacccaagct acgatttcgc catgatcccg ttttcgcagg
      181 gaatattacc gcgcattcgt ttcggtaaat ttccggaaag cggccgtgtc gccgttgaag
      241 acatcgagat cgacgcgttc actgatccct tcggcatgcg cattcgaagc gaattgccag
      301 aacgtccact tccttccgtt aagctcttcc gggcgggaat aaacgttccg tatccagacc
      361 gggcaggagg aaaaacgcga caggctgtaa tcggtataga aatcgcttgt gcagtaaagc
      421 acgggcgcga caccgtaata atttttcagc gagtcgatgc agtcgccgat ctcctgtatg
      481 atctgcgttt tcggtttcgc cgttttgcag ttgccgctgt attccaggtc gagcacgggc
      541 ggaagttttc ccggcaggtg cggaacgact tcgataaaat gtgcggcctg ctcgttcccg
      601 ttcctgcaga aggaataaaa atgataggcg cccacgatga aattgttttc ctgtgcttct
      661 gcgcaggatg cggaaaagct gggatcggtg tagtcgccgc cttccgtggc tttcatgaag
      721 acgaaggaaa agttttcagc gcgcagcttc tgccagtcga tgacgccgtt gtggtgcgag
      781 atgtcgatgc cgcggacggg aaattctttt tccgaagggt aattgaagcg tatgtgaaaa
      841 cggaagagcc acagcaatgt tatcccggtt gcgagcagga cgacaagcgc ggcgataatg
      901 atcttccgtt tcatctatcg tttggcgttg gatgaaaaat agcgcaccat gcggagactg
      961 tcggccggcg tataaaacca aaattccacc tgcgggattt ttttatacgg tgccgggagg
     1021 tcacgcagca gtgaatcatg cggggatgaa ccgcgacggg ttttcgccag gtttgttaac
     1081 gaaagaaccg tgtcaggcca gtcataatct acgccttctg tttccacgag cgagtcgccg
     1141 gaccacctgt attttgtgag gcccgcgggt tggggcgggt acggaaaccc gtagtccatg
     1201 caataaacac tttttgacgc gctgtcgaaa taaggattca tgacatacac atcgttatcg
     1261 cgtaaggaat gcattcccgg atcgtagaaa taaacatgat aattctcgcg cggacaacag
     1321 ccacagggtg aataaatgcc gagaagataa tcggcgtacc cgtcaaagtc ggcatcgagg
     1381 acggtgtccc acggactcca gtcccatcct tcctcgtcgc tgatgacaag cctgtagcgt
     1441 ttattctttt tgtaaacgcg cgtgtatacg ctgtctcctt tttgcgtgcg gatcacccag
     1501 aaggaatcgc tgccgcaggc aaaggcgcgg aattcctgtt tcacgatcgt gtcatgccga
     1561 tggaaaaacg catcctgccg catgatccgg aggaatgtgc tgtcgggaac tgttttttgc
     1621 cggggaggtt ctgaagtatt catgcggatg aaggaaaacc gccgcatgac gctgtccaca
     1681 ctttttcccg gtggagatga tttttcatcg caggaggcga agaccagcag gaagaaaaga
     1741 aaacagggta tgcgcttcac ttctttttca gtttcgcctt ctgcgcctcg ctgtgttttt
     1801 tcatttcggc gagagaagtg ctgagcaact tctcaagaac ttttttgtct atgtctgcaa
     1861 ggcgatcgat gtacaggcag cctttgcccg ttttgtgttt tccgagtttt ttgaggaggg
     1921 aagtgaatct cgccagtccg cccattgaat aaagcgtgag gttctgctta cgcggcgaaa
     1981 atcccatctt aaaccagtca ccttcacggc cgctgtcata gacataccgg taatcaccga
     2041 aaccgacgat cgcggggccc cacattttcg gttccgcttt cgtcaccttc ttcatcagct
     2101 tcacgatcgc gtggcagtcg cttctgcgtt cttcgtcttt gatcgcgtcg aggaaatctt
     2161 ccacgcttgc ggtggttttt tttgttttca gcccggccat gggaacaggg ttaaaatgga
     2221 tatgatcaaa tttaggaaag cgcggcgtga gatgaaagtt aattcacggt aatcactgtt
     2281 ttccttccat ggcagcgatc agctcatcgc gcgatttgcc gtagaacttc cgcgccaggt
     2341 tacgggaaag cgtatcctgc cggttggcga tcacctttgc gagaacggaa tcgccttcac
     2401 tggtgtgata cgcgacgata aggctcagcc cgtacatcgt catcatggaa taatatttcc
     2461 ggctggtgac cgggctcgtg tcggtgatcg agcggaagag agcgatgccc tcatcgtaat
     2521 atttttctgc gagcgccgta tcgcccgtgt gctgcgcaag cattcccgcc gtgatgcggt
     2581 gatcgggatt gccgggacgc agccggatca gctcgcgcgc agtggcaagc gctttcgcat
     2641 agaagtggag ctccgtttcg caggcgagct tgttccagta cgcgatgtaa tagttcgaat
     2701 cgagcgaaac ggcttcgtca tagagcatgt cggcgtacgc ataggaaaga ctgtcgccgc
     2761 gggcggagat cttcgtggcg ctgtcgttga gcgcgacggc cggaggtttt tgctgcggct
     2821 gtttttccgg ttcactgtcg catgcggaga cgagcaggag caggaacagc agtggaaatg
     2881 caaaagtttt catggggttg atgtgtttcg gatgtaacgt gttttgtgaa tgaaaagtac
     2941 gtttcattta ttttaccgca cgttttcgca tgatcatgcg cacgaggcca ccggtgatcg
     3001 cgagcagcgc aaaaatgtcc atgatcgtta cgatgtaatc gccgtgctcc gtgtagaacg
     3061 tcattcccgc cgtggaaacg atcttctgcc ggatcgctga agcggtccac cagggctggc
     3121 gctggaaaaa atccccgcgc tggtcgatga agcaggaaat gcccgtgttc gccgcgcgtg
     3181 caatgctctt gcgtgtttcg atggcgcgca gccttccgta aagcaggtgc tgcttgtagc
     3241 cgggcgtatt gccccaccat ccgtcgttcg tgatgatgaa aatataatcg gctccgttgc
     3301 ggatgtattg cgaaacgtaa tccccgtaaa tggactcgta gcagatcacg ggcgccactt
     3361 ttccgggtcc gttcggcgaa cggaaaacgg tgcgttcatc ctgcatgccc agcgagccca
     3421 ctgttccgcc gagatcgatc gcgtatttgt cgaggaagcc gaggtactgc gggaacggca
     3481 ttttctcaac gccgggcacc agcttcgatt tgtggtagag ctgtatcgcc tgcgccgagt
     3541 cgatctcgag cgcggtgtta tagttgtcgt aatattcatc gccatcggaa aatttccgcg
     3601 cggtggagga aagcttttcg ccggccttgt agtaatagcc ggttgtcgcg ccggtgacga
     3661 tgttcagctt cgggtaacgc tcacggaatt tgcgcaggcg gtgaatgctc cacgaagact
     3721 gtatgtcgtt ctcccagatg ctttccacga gcgctgtttc gggcatgacg acataatcga
     3781 cggaagaatc cgttttggca gcggcaagcg aaagcatttt ttccgtttca tcgcggaaat
     3841 cggaagtgaa cttctggtag ggatcgatgt tcggctgtac gacgacgaca tcgagcgggt
     3901 aggttgtatt tgcattgaca tcgaagcgga aataaataat gtacgaaaca agaacaggaa
     3961 gcagcagtac ggataaggtt accgccacca ggattttccg cgcttttttc tcgttttcga
     4021 gatttcttct cctgaggaat tcaaacagca gcacgttgat gagcaggatc cagaagcttc
     4081 cgccgaaaac gccggtgtat tcgtaccact gtatccacgt gtagttgttc acgagcccgt
     4141 tgcccagcgt cagccagggc caggaaagat cccagtcgag atgaaaatgc tcgaagatga
     4201 tccagaacgg gatgagcacg aacgtcccgt acttttccgg gagcatgcgt ttcaccttgt
     4261 gaaaagcgaa gaacacgat
//
LOCUS       DASTSF010000526         4919 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1490595,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000526 DASTSF010000000
VERSION     DASTSF010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4919
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1490595"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..319)
                     /locus_tag="VFU15_15005"
     CDS             complement(<1..319)
                     /locus_tag="VFU15_15005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719150.1"
                     /translation="MKRFLTLLFFFSILAQGFSQNVALDRRPVWLGLNMGGTFQTSDM
                     KPVAGIGWGATISRYSRLSKPGALYFGWRFRFLDGRDYGYNYHPLTGLNSDPVLSSSP
                     NYAD"
     gene            552..1139
                     /locus_tag="VFU15_15010"
     CDS             552..1139
                     /locus_tag="VFU15_15010"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU4719151.1"
                     /translation="MVELNNEIKKLSDEALVARFREKGDSICVGELFSRYRHLVFGVC
                     MKYLSDEDESQDVQMQVFEKLLRDLGLHQVEQFKGWLYAVTKNECLMYIRSRKSRRAK
                     EDELKKDMKGLMENENFLHPDGVTETEIQLREMESGLGQLGDNQRRCLELFYLEKKCY
                     REVSEITGYSMNEVKSNIQNGKRNLKLLLANRKHG"
     gene            1132..2346
                     /locus_tag="VFU15_15015"
     CDS             1132..2346
                     /locus_tag="VFU15_15015"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEU4719152.1"
                     /translation="MADEIKYIKLTGHECLNREKMFAYIDGQLSPADMHEVEAHLLDC
                     PFCSDALEGLEKVKDRHRVPAAVPPLAENAPVKKRGRILPLFPFANVVYGAVAGVLLV
                     VGIITLVPLLFQNGDNASKKLAQNTSEKKNEDSSVVMAREDRALDSVAAANIPAMDAD
                     RAAAQSRYESSGGDGPADVSDSGRNMFADVSADKNRESSAEEKWPAHAGLPKSSGNTG
                     NDVTNDLAQSKAVQEDDDQAQKTAANDYVAQDESKNKDLDKPDNFSNAGGSKKENHAR
                     SNAASAAAPPAQANVNISPAYNSASGASDVVQDSVSSGTATLLSDADIELYYRNGVSM
                     YDSGQLNASIAFFDKVLQYPQNKNYEDAQWMKAQALARTGKTDDAKALLNEIIEAKGK
                     HKKDAEKLLKTL"
     gene            complement(2347..2736)
                     /locus_tag="VFU15_15020"
     CDS             complement(2347..2736)
                     /locus_tag="VFU15_15020"
                     /inference="COORDINATES: protein motif:HMM:NF020903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1987 domain-containing protein"
                     /protein_id="HEU4719153.1"
                     /translation="MDVLFAAATKSTPELACDPVKGSISIKGRSIPADASVFYDRMDK
                     WVEKLCEEPGGRTVTFSVMVDHLNTGSVRSILTMMSRLKKAEAKGLKLVIDWNYEMDD
                     EDIRDKGEEMSMIVGHPFNYIPFRYEG"
     gene            complement(2745..3284)
                     /locus_tag="VFU15_15025"
     CDS             complement(2745..3284)
                     /locus_tag="VFU15_15025"
                     /inference="COORDINATES: protein motif:HMM:NF038262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SiaB family protein kinase"
                     /protein_id="HEU4719154.1"
                     /translation="MLTAQDVKGLLLDPATDWFYLGEIDEDLISSFVTLIENRPDFLL
                     LPRPSRRRLVSAVVECLQNIQNNTPVITEPKSGSVIFMQKYSLSEFGVMCGNIMNTSE
                     VEMLKEKLDQINSADTEILREMYMKQMEISLQGGSRTNAGLGLLEIARAVNGPIRYGF
                     IRLNDDHTFFSLELHLSLN"
     gene            3553..4314
                     /locus_tag="VFU15_15030"
     CDS             3553..4314
                     /locus_tag="VFU15_15030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEU4719155.1"
                     /translation="MSEQGYVRSSTENGICTITFFHPQSNSMPGKLLRALAAEISSAG
                     ENTDVRVIVLRSEGEKAFCAGASFDELISISDKETGEQFFSGFALVINAMRKAPKFVI
                     ARVQGKAVGGGVGIASAADYAMATDAASVKLSELAVGIGPFVVGPAVERKIGTSAFTN
                     LAINATEWRDAAWAKNCGLYADVYSSVAELDAAVAALAQKLSLSNPEAMNELKKIAWA
                     GTDHWDLLLFERAQISGRLVLSDFTRNAISKFKAK"
     gene            complement(4325..>4919)
                     /locus_tag="VFU15_15035"
     CDS             complement(4325..>4919)
                     /locus_tag="VFU15_15035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719156.1"
                     /translation="LFYDHKKLDHIITDAAQAEIYDLVKVDYNVDDIGAAYDTLRKVA
                     VSVIDMKRETYTNMGFKTTILTMAEGYDSKYPDERYESYTAYLQDASATNVPQQNPNV
                     QIRQADKEQTIFYNKVPYKQFDKVLNADIAEPGVQFFYKLRVKCRMEPIADDGNTNAK
                     TNSAKTGNAAPPAAGTYKPQTHVSPAQQAHAPVVPGH"
BASE COUNT         1251 a         1247 c         1296 g         1125 t
ORIGIN      
        1 gatccgcata gttcggagaa gaagaaagca caggatcgga attaagtcct gtaagcggat
       61 ggtaattgta cccgtagtct cttccgtcga gaaaacggaa gcgccatccg aaatacaatg
      121 cgcccggttt gctgagcctg ctataacggg agatggtagc cccccagcct atgccggcca
      181 ccggcttcat gtcgcttgtc tggaaagttc ctcccatgtt gaggccgagc cataccggcc
      241 ggcgatccag cgcaacgttc tgcgaaaaac cctgcgcaag gatggagaag aaaaagagga
      301 gagtaaggaa tcgtttcata accagtggat ttaagtggac acaacccgtt ttgcaggaat
      361 tgtgccgaag gcttgggacg gtcagtggtt aaaattgtta aggctgattt ccaatcgttt
      421 aggaatggaa gggttttgta tctggcaaat ctaaccattc ccttccgcat atcaaatggc
      481 ccggaagttg cgccaggccg cttatttatt cccgtccggc acaaaggcaa tttgtaattt
      541 tatcccgtcc cgtggtcgag ttgaacaacg aaataaaaaa actgagcgat gaagcgctcg
      601 ttgcccgttt ccgtgaaaaa ggagacagta tctgcgtggg agaactcttc agccgttacc
      661 gccacctcgt tttcggtgtc tgcatgaaat atctttccga tgaggacgaa agccaggatg
      721 tccagatgca ggttttcgaa aagctgctcc gcgacctcgg ccttcaccag gtggaacaat
      781 tcaaaggatg gctttacgca gtgacgaaga acgaatgcct catgtacatc cgcagcagga
      841 aaagcaggcg cgcgaaagaa gacgagctga aaaaagatat gaagggactt atggaaaatg
      901 aaaatttcct gcatcctgat ggcgtaaccg aaaccgaaat ccagctacgc gaaatggaat
      961 caggactcgg gcagttgggc gacaatcagc gccgctgtct cgaactgttc tacctggaaa
     1021 aaaaatgcta ccgggaagtt tcggagataa ccggttactc gatgaatgaa gtgaaaagta
     1081 atattcagaa cggaaaacga aacctgaagc tgctgcttgc aaacaggaaa catggctgac
     1141 gaaatcaaat atataaagct caccgggcac gaatgcctca accgggaaaa aatgttcgcc
     1201 tacatcgacg gacagctttc tcctgccgac atgcatgaag tggaagcgca tctcctcgac
     1261 tgcccgttct gcagcgacgc gctcgaaggc cttgaaaaag taaaggaccg ccaccgcgta
     1321 cctgctgccg ttccgcccct tgctgaaaac gcccccgtta aaaaacgcgg ccgcatcctt
     1381 ccgctgttcc cgtttgccaa tgtcgtttac ggcgccgtag cgggtgtgct gctggtcgtc
     1441 gggatcatca cgctggttcc gcttcttttt cagaacggcg ataatgcgtc gaaaaagctg
     1501 gcgcaaaata ccagcgaaaa gaagaatgaa gattcatctg tagtgatggc ccgtgaagac
     1561 cgggcgctcg attccgtagc tgcggccaac atcccggcca tggatgccga ccgtgctgcg
     1621 gcacagagcc gttatgaatc ttccggtggc gacggaccgg ccgacgtgag cgattccggc
     1681 aggaacatgt tcgccgatgt ttccgcagat aagaaccggg aatcttctgc agaagaaaaa
     1741 tggcctgctc atgccggttt accgaagagc agcggcaata cgggaaatga tgtaaccaat
     1801 gatcttgcac aatcgaaagc cgtgcaggaa gatgatgacc aggcgcagaa aacagcagcg
     1861 aatgattatg tagcgcagga tgaaagcaag aacaaggacc tggataagcc tgataatttc
     1921 agcaatgcgg gcggctcgaa gaaagagaat catgcgcgca gcaatgctgc aagcgcagcc
     1981 gctccccctg cacaggcgaa cgtaaatatt tcgccggcgt acaattccgc atcgggcgct
     2041 tctgacgttg tgcaggattc agtgagctca ggaaccgcca cccttctttc cgatgcagat
     2101 atcgagcttt attaccgcaa cggcgtgagc atgtacgact ccgggcaact gaacgcgtcg
     2161 atcgcattct tcgataaagt tcttcaatac ccgcagaata aaaattacga agatgcgcaa
     2221 tggatgaaag cgcaggccct ggccagaacg ggcaaaaccg atgatgcgaa agcgctgctc
     2281 aacgagatca tcgaggcgaa aggcaagcat aaaaaagatg cggaaaaatt gctgaagacg
     2341 ctgtgatcag ccttcgtagc ggaacgggat gtaattgaac gggtgcccga cgatcatgga
     2401 catctcctct cccttgtcgc ggatatcttc atcgtccatt tcgtaattcc agtcgatcac
     2461 gagcttcagt cctttcgctt ccgctttttt caggcggctc atcatcgtca ggatcgacct
     2521 cacggaacct gtattgagat gatcgaccat gacactgaac gtaacggtac gcccgcccgg
     2581 ttcctcgcat aatttctcga cccatttgtc catgcgatcg taaaaaacgg aagcgtctgc
     2641 aggaatggat ctccctttaa tactgatact gccttttacc gggtcacatg cgagctccgg
     2701 tgtcgatttg gtggcggctg caaacagaac atccatacat cgaatcagtt cagggagaga
     2761 tgaagctcga ggctgaaaaa tgtatgatcg tcattcaggc ggataaaccc gtaacggatc
     2821 ggtccgttca cggcacgtgc aatttcaaga agtccgagcc cggcgtttgt ccggctcccg
     2881 ccctgcaatg aaatttccat ctgcttcata tacatttcgc gaagaatttc cgtgtctgcg
     2941 gaattgatct ggtcgagctt ttccttcagc atttccacct cggatgtgtt catgatattt
     3001 ccacacatga cgccgaattc actgagtgaa tatttctgca tgaagatcac cgagccgctt
     3061 ttcggttccg tgatgaccgg cgtattgttc tggatattct gaagacattc caccactgcg
     3121 ctgaccaacc ttctccgtga aggccggggc agaagaagaa aatccgggcg gttctcgatc
     3181 agtgtaacaa aagaacttat caggtcttca tcaatttcac caaggtaaaa ccaatcagtt
     3241 gcaggatcta gcagcagacc ttttacatct tgcgctgtga gcatatcggg ggattaataa
     3301 tgcttttacg gtaaaattaa ggaaatggtt atcaccggaa acttattttt ggatattgaa
     3361 taaaatttgc gcccggcaaa aaaactgcga ccaggtccgg aaagattacg ccgcttttcc
     3421 cgcaacgaac ggaggtacaa acgacgaacg aaccatggct gaagttgaac ccggtcagcg
     3481 gaaggtaccg aaacagggcg cggaaaaaac acgttgcatt ttagttgtta gttttgcgca
     3541 aactttttca ccatgtcaga acagggctat gtaagatctt ccactgaaaa cgggatctgc
     3601 accattactt tttttcatcc gcagagcaat tccatgccgg gaaagctgct gcgtgcgctg
     3661 gccgcagaaa tatcttccgc cggtgaaaat accgatgtgc gagtgatcgt cctgcgcagc
     3721 gaaggggaaa aagctttctg tgccggcgct tctttcgatg agctcatcag catcagcgac
     3781 aaagaaacgg gcgagcagtt tttctcgggc ttcgcgcttg tcatcaatgc catgcggaaa
     3841 gcgccgaaat tcgtcatcgc gcgtgtgcag ggaaaagcgg taggcggcgg tgtcggtatt
     3901 gcttccgctg ccgattacgc gatggcaacg gatgccgcat cggtgaagct gtcggagctg
     3961 gccgtgggca tcgggccgtt tgtcgtcggt cccgccgtgg agcggaagat cggaacatcc
     4021 gcattcacca atctcgcgat caacgcaacg gaatggagag atgcggcctg ggcaaaaaac
     4081 tgcggacttt acgctgacgt ctattcttcc gtcgctgagc tggacgctgc cgttgcggcg
     4141 ctggcccaaa aactttcact cagcaatccc gaggcgatga atgagctgaa gaagatcgcc
     4201 tgggccggga ccgatcactg ggaccttctt cttttcgagc gcgcgcagat cagcggccgg
     4261 ctcgtgcttt ccgattttac gcggaacgcg atcagcaaat tcaaagcgaa ataaatcctt
     4321 cttttcagtg ccccggcacg acaggcgcat gcgcctgctg tgcaggagaa acgtgcgtct
     4381 gcggtttgta agtccctgca gccggaggcg cagcgttgcc ggtcttcgct gaattcgttt
     4441 ttgcattcgt gttgccgtca tccgcgatcg gttccatcct gcatttcaca cgcagcttgt
     4501 aaaaaaactg cacgccgggt tccgcgatat ccgcgttgag cactttgtcg aattgcttgt
     4561 acggaacttt gttgtagaag atcgtctgct ctttgtccgc ctggcgaatc tgcacgttcg
     4621 ggttctgctg cggaacattc gtcgccgagg catcctggag atacgccgtg tagctttcat
     4681 aacgctcatc aggatatttg ctgtcgtagc cttccgccat agtaaggatc gtggttttga
     4741 aacccatgtt cgtgtatgtt tcgcgtttca tatcgatcac ggaaacggcg accttgcgca
     4801 gcgtgtcata cgccgcaccg atatcgtcga cgttgtaatc cactttcacg aggtcgtaaa
     4861 tttccgcctg cgcagcatcg gtgatgatgt gatccaattt tttgtgatca taaaaaagg
//
LOCUS       DASTSF010000527         3166 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1898849,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000527 DASTSF010000000
VERSION     DASTSF010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3166
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1898849"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(36..1106)
                     /locus_tag="VFU15_15040"
     CDS             complement(36..1106)
                     /locus_tag="VFU15_15040"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014772939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HEU4719157.1"
                     /translation="MNTATMTIDVTKTQHSRLPGLDINNLPFGKVFSDHMFIADYEEG
                     KWGNLRILPYGDLHLSPASSMMHYGQAIFEGMKAYRSDEGDILLFRPRDNQRRLNISA
                     HRMAMPDVPEEIFMDGLDELLRLDQNWIPGGDGRSLYIRPFLIATDNYIGVKPSDTYK
                     FLIITTPVGAYYDHPLKVKVEKTFFRAVAGGVGHIKVSGNYGRSLYPSSLAKQEGYDQ
                     LIWTDAKEHKYVEESGTMNLMFVIDDVLITPQLGQTILAGITRDSVLTLARDWGMKVE
                     ERKISVDEVVEAQKEGRLQEAFGTGTAATIAHISNLGYDGTDYQLPPVDERVFSNKVS
                     RALNDLRRGRIPDEHNWIHKVC"
     gene            1448..1957
                     /locus_tag="VFU15_15045"
     CDS             1448..1957
                     /locus_tag="VFU15_15045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719158.1"
                     /translation="MKELPPVRHSDHTAGTWYSYFELTPVFYSSSMGGNLQQLRCSGL
                     YKGNKVSVDIVFDYTKGNSGNRISFVRTGDESDRLQAAMAELYDEELYAGPMKDTVSC
                     SFYDGEPDDISLESGVHVYKMNFPAGKTGEPGSVEINLHVDLAKGIVRFGEKNSGMAR
                     LNFVQAFRK"
     gene            1978..2289
                     /locus_tag="VFU15_15050"
     CDS             1978..2289
                     /locus_tag="VFU15_15050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719159.1"
                     /translation="MKESESLFLTLAEEIAGVTKGKMFGALCMKTAKGKSAAMLWKGD
                     IVVKLPAAEIARTMKLKGACHFEPMKGRPMKEWVQVPPAHSARWKELVKKSLAFVESE
                     K"
     gene            2331..3110
                     /locus_tag="VFU15_15055"
     CDS             2331..3110
                     /locus_tag="VFU15_15055"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU4719160.1"
                     /translation="MTTPKTILFIHGMFMNSSCWDDWMRFFSGKGYSCLAPSWPGHEG
                     SVKELREKHPSASLEKLTLGDVVRFFEEKINGLPGKPVLIGHSMGGLAVQLLLNRGLA
                     SAGIAVDPAPPKGVFSFKWSFLKSNLPVVNPLKGNRPDLMSLKRFRYAFVNGMSESEQ
                     ENAWTRYVVPESRNVPRSSTKNDGKADFRKAHDPLLIIAGGNDHIIPPSLNRKNFEKY
                     KAVGSVTEFKEFPGRNHFTIGQPGWEEVADFCEKWINRNVK"
BASE COUNT          760 a          797 c          833 g          776 t
ORIGIN      
        1 ttgaaaaaga aaaccggtag atcatctggc ggagatcagc agactttgtg aatccagttg
       61 tgctcgtcgg gaattctgcc gcggcgcagg tcgttcagcg cgcgggaaac tttgttcgag
      121 aacacgcgct catcaacagg cggaagctgg taatcggttc catcgtaacc gagattggaa
      181 atgtgtgcga tcgttgcggc ggttcctgtt ccgaaagctt cctgtagtct tccctccttc
      241 tgcgcctcca ccacttcgtc cacggaaatt tttcgttcct ccactttcat tccccagtcg
      301 cgcgcaagcg tcagcacgct gtcgcgcgta atgcccgcga ggatcgtttg tcccagctgc
      361 ggcgtgatca gcacatcgtc gatcacgaac atcaggttca tcgttccgga ttcctccacg
      421 tatttgtgct ctttcgcgtc ggtccagata agctggtcgt aaccttcctg ctttgcgaga
      481 ctcgacgggt aaagcgatcg tccgtaattc ccggaaacct tgatgtgccc gacgccgccg
      541 gcgactgcgc ggaaaaaggt cttttccact ttcactttca gcgggtgatc gtaataagcg
      601 ccgaccggtg ttgtgatgat caggaatttg taagtatcgg atggcttcac gccgatgtaa
      661 ttatccgttg cgatgaggaa agggcgaatg taaagcgaac gtccgtcgcc gcccgggatc
      721 cagttctggt cgagcctgag cagctcgtcg agcccgtcca tgaaaatttc ttccgggaca
      781 tccggcatgg ccatgcggtg cgctgaaatg ttcaggcggc gctggttatc cctcgggcgg
      841 aaaagaagaa tgtcgccttc atcggaacgg tacgctttca ttccctcgaa aatagcctgg
      901 ccgtaatgca tcatggagct ggccggactg agatggagat caccataagg aagaatgcgg
      961 agattccccc atttcccttc ttcgtaatcg gcgatgaaca tgtgatcgga aaacactttt
     1021 ccgaaaggaa gattgttgat gtccagcccc ggcaaacggg aatgctgcgt cttcgtcaca
     1081 tcgatggtca tagtggctgt gttcattggt tcctttcttg tcaaggggat agcggcaaat
     1141 atgccaatta atatccggaa taattaccgg tgaaacttat tttcgacggg ttcaggatta
     1201 ttgttgacaa agcggtaatt tagtaatgca tgagaaaaac attgctttat acggcggtcc
     1261 tcgcattaat gttctgctgc ggatgcatgc acggcaaaca ttcgcagaaa aaatccaaat
     1321 acgcaccctg cgactgcacc aaagtggatt tccgatgaaa ttctgccgca tcacaagcat
     1381 ctcttgtttt ttcctgtctt cccttttttt gttttcctgc ggcagtcacc gctcctttga
     1441 tttcccggtg aaagagcttc cccccgttcg tcacagtgat cataccgccg gcacctggta
     1501 cagctatttc gagcttactc ctgtttttta ttcctcatcc atgggcggta acctgcagca
     1561 gctgcgctgt tccggccttt acaagggaaa caaagtttcc gtggatattg ttttcgatta
     1621 cacgaaaggg aacagcggta accgcatctc ttttgtgcgc accggcgacg aaagcgaccg
     1681 gctgcaggcg gccatggctg aactttacga cgaagagctt tatgcgggac cgatgaaaga
     1741 cacggtttcc tgcagctttt acgacgggga accggacgat atttcactgg agagcggcgt
     1801 tcatgtctat aaaatgaact tccccgccgg aaagaccgga gagccgggat cggtcgagat
     1861 caatcttcat gtcgatctcg ccaaagggat cgtccgtttc ggggaaaaga attcaggaat
     1921 ggcccgcttg aattttgtcc aggcgttcag gaaataaaaa atgtaaatta atttttcatg
     1981 aaagaatcag aatcgctttt tcttactctc gctgaagaga tcgcaggtgt gacaaaagga
     2041 aaaatgttcg gcgcgctctg catgaaaaca gcgaagggga aatctgccgc catgctgtgg
     2101 aagggcgata tcgtcgtcaa gcttccggcg gcagaaattg ccagaacgat gaagctgaaa
     2161 ggcgcctgtc atttcgaacc gatgaaagga cgcccgatga aagaatgggt gcaggtgcct
     2221 cccgcccatt ccgccaggtg gaaagagctg gtgaaaaagt cgctggcgtt cgttgaaagc
     2281 gaaaaatgaa caaccgccgg aattaataac cgcgcttaat aaataccggt atgaccacgc
     2341 cgaaaacgat ccttttcatt cacgggatgt tcatgaactc ttcctgctgg gacgattgga
     2401 tgcggttttt ttccgggaaa ggctactcct gcctcgctcc ttcctggccc ggacacgaag
     2461 gaagcgttaa agagcttcgc gaaaagcacc cgtctgcttc gctcgaaaaa ctgacgctcg
     2521 gcgatgtggt ccgctttttc gaagaaaaaa taaacgggct ccccggaaaa cctgtgctca
     2581 tcgggcattc gatgggcgga cttgccgtgc agctgctgct taaccgcgga ctcgcttctg
     2641 ccggcattgc tgtcgacccg gctccgccca aaggtgtttt ttcgttcaaa tggtctttcc
     2701 tgaaaagcaa tcttcctgtc gtcaacccgc tgaaaggaaa tcgccctgac ctcatgagcc
     2761 taaaacgctt ccgctatgcg tttgtgaacg ggatgagcga aagcgaacag gaaaatgcgt
     2821 ggacaagata tgttgttccg gaatcgcgca acgtgccgcg cagctccacg aagaacgacg
     2881 ggaaagcgga tttcaggaaa gcgcatgatc cgttgctgat cattgcgggc gggaacgatc
     2941 acatcattcc tccttcgctg aacaggaaga actttgagaa atacaaagcc gtgggttccg
     3001 tcactgaatt caaagaattt ccaggcagga atcattttac catcgggcag ccgggctggg
     3061 aagaagtagc ggacttctgc gagaaatgga taaacaggaa tgtgaaatga aacgcggtac
     3121 cgcagctttt atgtcatttt tcgtgttgtt tttttcctgc cgatcg
//
LOCUS       DASTSF010000528         4205 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1660314,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000528 DASTSF010000000
VERSION     DASTSF010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4205
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1660314"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            308..1102
                     /locus_tag="VFU15_15060"
     CDS             308..1102
                     /locus_tag="VFU15_15060"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011584676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEU4719161.1"
                     /translation="MKQYHDLMRHVLEHGAKKTDRTGTGTVSVFGYQMRFDLAEGFPV
                     VTTKKLHLRSIIYELLWFLRGETNIKFLRDNGVSIWDEWADENGNLGPVYGYQWRSWP
                     APDGRHIDQVSNVIEQIKRNPDSRRLIVSAWNVGEIEKMKLPPCHAFFQFYVADGKLS
                     CQLYQRSADIFLGVPFNIASYALLTLMVAQVCNLQPGDFVHTLGDAHLYSNHIEQAKL
                     QLSRELRPLPQMKINPEVKNIFEFRYEDFTLEGYDPHPHIKAAVAV"
     gene            1112..1681
                     /locus_tag="VFU15_15065"
     CDS             1112..1681
                     /locus_tag="VFU15_15065"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006988228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEU4719162.1"
                     /translation="MNDQAWRRTAIIICHTTFLILKIAIIVAASENNAIGKDNRLIWR
                     LPDDMKFFREKTSGHCVVTGRKNYESIPGKFRPLPARTNIVVTRQENYSAPGAIVVHS
                     FGDAVAKAKELGETELWVIGGGEIYREALPVTDIVWLTRVHHHFDAHTFIPGLDPSQW
                     EMTWKEEHPADQNHAYAFTFYKYERRKSL"
     gene            1704..2132
                     /locus_tag="VFU15_15070"
     CDS             1704..2132
                     /locus_tag="VFU15_15070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719163.1"
                     /translation="MKKLLGISFAVLVALIGFVTVLWLLPCNGDCCGKDHCTGNEQCM
                     RDHDGMMGDHDCGDGKCERKEIKVFVDKDGKCDGDEKCSMGGMDKCEMNGMGMMNGMG
                     CCCCCRMMCGGMGMDDKCMKGDSMKCDMKSDSMKCCKKNK"
     gene            2219..2746
                     /locus_tag="VFU15_15075"
     CDS             2219..2746
                     /locus_tag="VFU15_15075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719164.1"
                     /translation="MITFSETQRFRQWWIWLSLAAMEAYIIYGFVQQIVLGHPFGSRP
                     APDWALYILICVPSLFVGLAVSLRLETKINGDGVFLRYKPFINTTRAFLWKDVGSAFV
                     RQYRPIAEYGGWGIRGNFYGRWNGKAYSVSGNMGLQLVMKNGKKILIGTCNAEELKAV
                     LQRLADKKLVINAEI"
     gene            complement(2816..3295)
                     /locus_tag="VFU15_15080"
     CDS             complement(2816..3295)
                     /locus_tag="VFU15_15080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719165.1"
                     /translation="MENPPVTSRVSTGLITGFIIWLMMGLGFIGISVIEFMMKSDHAI
                     VELVVGVIFLGLVSLASTRLKSFRVENNTLYVTHVISRKVTALTEKNIAEATALLNMR
                     QMTTAGMLLNRRFHQLVITPVEGKRFIISSKGATRFMELVHALEKMLGKKLVIEDHG"
     gene            complement(3340..>4205)
                     /gene="clpX"
                     /locus_tag="VFU15_15085"
     CDS             complement(3340..>4205)
                     /gene="clpX"
                     /locus_tag="VFU15_15085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEU4719166.1"
                     /translation="ARTIAKLLNVPFCIADATVLTEAGYVGEDVESILVRLLQAADYD
                     VSAAERGIVFIDEIDKIARKSDNPSITRDVSGEGVQQALLKMLEGSVVNVPPQGGRKH
                     PEQKMIQVNTKNILFVCGGAFDGIDKKIARRIKTQTIGYTSSTEDTHIDKDNLLQYIV
                     PQDLKTYGLIPELIGRLPVLAHLNPLDAEALRRILTEPKNALTKQYTRLFDMDGVKLS
                     FDDAVFDHIVEKAMEYKLGARGLRSICEAIMIDAMFDIPSEGTTKRLHITLDYAKEKL
                     SKAKMEKLKAA"
BASE COUNT         1003 a         1064 c         1160 g          978 t
ORIGIN      
        1 cgttgagatt gcccggggga taggcgatca cctggtaatt ggtgggatcg ctttcgaagt
       61 tatcgaagag cagcgtgtcg ttctggctga atgcaggagc aacaagcatg atcagcagaa
      121 aaagaaagta gaattttttc atggttttga taattggtga gcaatgcgaa gttacggaat
      181 accagcggga atagccattt atgaaccgtg gcgggtcatt cgggaaactg gttgaaaaaa
      241 tgcggaaaac cttgtcctgc acggataaca caacgctctt cacttcttaa ttttgttttc
      301 aattccaatg aaacaatacc acgacctcat gcgccatgtt cttgaacacg gtgcaaaaaa
      361 aacagaccgg acgggaacgg gaacagtttc cgttttcggt taccagatgc gcttcgatct
      421 cgctgaaggc tttcccgtcg tcaccacgaa gaagctgcac ctgcgatcga tcatttacga
      481 gctgctctgg ttcctccgcg gcgaaacgaa cataaagttc ctcagagaca acggcgtctc
      541 catctgggac gaatgggcag acgaaaacgg gaacctcggt cctgtgtacg gctaccagtg
      601 gcgctcgtgg cccgcaccgg acgggaggca catcgaccag gtctcgaacg tcatcgagca
      661 gatcaaaaga aatcccgact cgagacggct catcgtcagc gcctggaacg tgggtgaaat
      721 agaaaaaatg aagctgccgc cctgccacgc gttcttccag ttttatgtgg cggacggaaa
      781 gctgagctgc cagctctacc agcgcagcgc ggatattttt ctcggcgtcc cgttcaacat
      841 cgcctcgtac gcgctcctca cgctcatggt tgcgcaggtg tgcaacctgc agcccggtga
      901 tttcgtgcac acgctcggcg acgcgcatct gtattcaaac cacatcgagc aggcgaaact
      961 gcagctttcc cgcgagctgc gcccgctgcc gcaaatgaag atcaacccgg aggtgaagaa
     1021 tatttttgaa ttcagatacg aagatttcac gctcgaaggc tacgatccgc atccgcacat
     1081 caaagcagca gttgccgtat aataattaat gatgaatgat caggcgtggc gaaggaccgc
     1141 gatcatcatt tgtcacacaa cattcctcat tttgaagatc gccatcatcg tcgcggcatc
     1201 cgagaacaac gccatcggca aggacaaccg gctcatctgg cgcctgcccg acgacatgaa
     1261 attctttcgt gaaaaaacgt cgggacactg tgtcgtcacc gggcggaaaa attacgaatc
     1321 cattcccggc aagtttcgcc cgcttccggc ccgcaccaat attgtcgtca cgcgccagga
     1381 aaattattcc gcccccggtg cgatcgttgt tcattctttc ggcgacgctg ttgccaaagc
     1441 gaaagagctg ggtgaaacgg agctctgggt gatcggcggc ggggaaatat accgcgaagc
     1501 gcttcctgtc accgatatcg tctggctcac gcgcgtgcat catcatttcg atgcgcatac
     1561 gttcatcccg ggtctcgacc cttcccagtg ggaaatgacc tggaaggagg agcacccggc
     1621 tgaccagaat cacgcgtacg cgttcacttt ttataaatac gagcgaagaa aatctttgta
     1681 aattcgttca aacctttatt tctatgaaaa agcttctcgg gatttccttc gctgtgcttg
     1741 tcgcacttat cggatttgta accgttctct ggctgcttcc ctgcaatggc gactgctgcg
     1801 ggaaagatca ctgcaccggc aacgagcaat gcatgcgcga tcatgacgga atgatgggcg
     1861 atcacgattg cggtgatgga aaatgcgagc ggaaagagat caaagtcttc gtcgacaagg
     1921 acggcaaatg cgacggggat gaaaaatgca gcatgggcgg aatggacaaa tgcgaaatga
     1981 acggcatggg catgatgaac ggaatgggtt gttgctgctg ctgccggatg atgtgcggcg
     2041 gaatgggaat ggacgacaaa tgcatgaaag gcgacagcat gaaatgcgat atgaaaagcg
     2101 atagcatgaa gtgctgcaag aaaaacaaat aggatcagca taaattcaat acggaatggc
     2161 ggaggatctc cgccattttc agtttccacc ctttttacat ttgataaaac cccgtttcat
     2221 gatcacgttc tccgaaacac agcgcttccg ccaatggtgg atatggcttt ccctcgcggc
     2281 gatggaggca tacatcattt atggattcgt gcagcagatc gtgctcgggc atccgttcgg
     2341 ttcacgcccg gcacccgact gggcattgta tattctcatt tgcgttcctt cattgtttgt
     2401 gggacttgcc gtttcacttc gccttgaaac gaagatcaac ggggacggtg ttttcctgcg
     2461 ctacaaaccg ttcatcaata cgaccagggc ttttctgtgg aaagatgtgg gatctgcatt
     2521 tgtcaggcag tacaggccca tcgcggaata cggtggatgg ggaatacggg ggaattttta
     2581 cggaaggtgg aacggcaaag cgtacagcgt ttccgggaac atgggtctgc agctcgtgat
     2641 gaaaaacggg aagaaaatac ttataggtac gtgtaatgcg gaagaactga aagcggtgct
     2701 gcagaggctg gctgacaaaa agctcgtgat aaacgcggag atctgatcac cccgggatcc
     2761 gggcgatgcg gaaaatcgcc gtgaatagct gtaagcgcaa aaccggttcg cacacttacc
     2821 cgtggtcttc gatgaccagt tttttcccca gcattttttc gagcgcgtgt acgagctcca
     2881 tgaaacgggt tgcgcctttt gaagagatga tgaaacgttt tccttccacc ggggtgatca
     2941 cgagctggtg aaaacgccgg ttcagcagca tgccggccgt cgtcatctgc cgcatgttga
     3001 gcagcgctgt tgcttctgca atattctttt ccgtcagcgc agtcactttc cggctgatca
     3061 cgtgtgtcac gtaaagcgta ttgttttcca cccggaacga tttcaatcgt gtggaggcga
     3121 gcgagacaag accgaggaag atcacgccca cgaccagctc gacgatcgca tgatcgcttt
     3181 tcatcatgaa ttcgatcacc gaaatgccga tgaagccgag tcccatcatc agccagatga
     3241 tgaagccggt gatcaacccc gtgctgacgc gactggtaac cggaggattt tccatagcac
     3301 aaaaaaagcg accgtggtcg cctggaatga gttaatgaat taagcggctt tcagcttttc
     3361 cattttggct ttgctgagct tttctttcgc ataatcgagc gtgatgtgga ggcgtttcgt
     3421 tgtgccttcc gaaggaatat cgaacatcgc gtcgatcatg atcgcttcac agatgctgcg
     3481 gagcccgcgt gcgccgagct tgtattccat cgctttctcc acgatgtgat cgaagaccgc
     3541 atcgtcgaaa gaaagcttca cgccgtccat gtcgaacagg cgtgtgtatt gttttgtcag
     3601 cgcgttcttc ggctcggtga gaatgcgtcg caacgcctcc gcgtcgagcg ggttgaggtg
     3661 cgcgagcacg ggaagcctgc cgatgagttc ggggatgagt ccgtacgttt tgaggtcctg
     3721 cggaacgatg tactgcagga gattgtcttt gtcgatgtgg gtgtcttcgg tggaagaagt
     3781 gtagccgatc gtctgcgttt tgatgcgccg cgcgatcttc ttgtcgatgc cgtcgaaagc
     3841 cccgccgcat acgaagagga tgttcttcgt gttcacctgg atcatcttct gctcgggatg
     3901 ttttctgccg ccctggggcg gaacattcac cacgctgcct tcgagcatct tgagcaacgc
     3961 ctgctgtacg ccttcgccgg aaacgtcacg cgtgatggaa ggattgtcgc tcttgcgcgc
     4021 gatcttgtcg atctcgtcga tgaagacaat accgcgttcc gcagcgctta cgtcgtaatc
     4081 ggcagcctgg agaaggcgga cgagaatgct ttcgacgtct tcgcccacgt aaccggcttc
     4141 ggtaagaaca gtcgcatcgg cgatacagaa cggaacgttc agcagcttcg cgatggtgcg
     4201 ggcga
//
LOCUS       DASTSF010000529         3898 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1884780,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000529 DASTSF010000000
VERSION     DASTSF010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3898
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1884780"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            29..259
                     /gene="atpE"
                     /locus_tag="VFU15_15090"
     CDS             29..259
                     /gene="atpE"
                     /locus_tag="VFU15_15090"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018710397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HEU4719167.1"
                     /translation="MDALGNGYGLAALGAGVAALGAGVGIGRIGGSAVEAIARQPEAM
                     GDIRANMILTAALVEGAAFFAMVVGLLVILTK"
     gene            372..863
                     /locus_tag="VFU15_15095"
     CDS             372..863
                     /locus_tag="VFU15_15095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004919945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEU4719168.1"
                     /translation="MLMVPDTGLAIWMLITFLIVLFLLTKFAWKPIMKMIREREESIE
                     GALKSAEKAKEEMQALQAGNERILAEARNERDRMMKEAREIKDSIVNEAKTKAKEEAD
                     KLLASARETIQNEKMAAITELKNQVAALSIDIAERILKRELASENKQKDLIGEMLKEA
                     KLN"
     gene            866..1423
                     /gene="atpH"
                     /locus_tag="VFU15_15100"
     CDS             866..1423
                     /gene="atpH"
                     /locus_tag="VFU15_15100"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HEU4719169.1"
                     /translation="MTGTRVSYRYAKSLLDLSLEKGQLEQVHEDMQLVLNTIRGSRDL
                     ELLLKSPVIKTDKKDDILKAIFGGKIGVITTAFIDILTRKRREGYLESVADSFVNQYK
                     KHKKILTAVVTTALGLDESLREQVVNVVKQGGKSEVELQEKVDANIIGGFILRVGDNQ
                     VDASIIRQIKNLERTFNENPYIKDF"
     gene            1481..3061
                     /gene="atpA"
                     /locus_tag="VFU15_15105"
     CDS             1481..3061
                     /gene="atpA"
                     /locus_tag="VFU15_15105"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007653339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEU4719170.1"
                     /translation="MAEVKPAEVSAILRQQLAGFKSAKEMEEVGTVLQVGDGIARIYG
                     LTNVQSVELIEFENGLKGVTLNLEEDNVGAVLLGDSKGIKEGDTVRRTGKIASLMVGE
                     GMLGRVVDALGNPIDGKGPIKGQTYEMPLERKAPGVIFRQPVNEPLQTGIKAIDAMIP
                     IGRGQRELVIGDRQTGKTAVCLDTIINQKEFYEAGNPVFCIYVAVGQKGSTVANVVRT
                     LEENGAMPYTVVVSASASDPAPLQFFAPMAGAAIGEFFRDTGRPALIVYDDLSKQAVA
                     YREVSLLLRRPPGREAYPGDVFYLHSRLLERAAKINASDEVAKNMNDLPESIRSMVKG
                     GGSLTALPIIETQAGDVSAYIPTNVISITDGQIFLESNLFNSGVRPAINVGISVSRVG
                     GNAQIKSMKKVAGTLKLDQAQFRELEAFAKFGSDLDAATKSVIDKGSRNVEILKQGQF
                     SPFRVEEQIAIIYCGTKGLLRDVPVNKVREFESDFLAYMKNKHADVLAALKAGKLDDT
                     ITAALESAAKEISAKYKA"
     gene            3074..>3898
                     /gene="atpG"
                     /locus_tag="VFU15_15110"
     CDS             3074..>3898
                     /gene="atpG"
                     /locus_tag="VFU15_15110"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007647353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HEU4719171.1"
                     /translation="MANLKEVRNRITSVSSTQQITSAMKMVSAAKLRRAQDAITQMRP
                     YANKLREILGNLSGSLDSSEGVYSRKREVRNVLLVVITSNRGLCGGFNSSVVKRAVRE
                     LSENYPSAKASYFCIGKKGGDFFRKTPHTAAFTDAENPNALFDNLTFANVAPVAERIM
                     KAFADGTYDKVVLIYNQFKNAAVQITQAEQFLPVVPNEGSKTLKTDYVFEPSRSEIVL
                     DLIPRSLKTQLYKALLDSHASEHGARMTAMHKATDNAKELIRSLRISYNKARQAAIT"
BASE COUNT          996 a         1092 c         1064 g          746 t
ORIGIN      
        1 aaatcttctt ctccaggctg ccgatcacat cgacgctctt ggtaacgggt acggtcttgc
       61 cgccctcggt gccggtgtcg ctgcgctcgg tgcaggtgta ggtatcggcc gcatcggcgg
      121 ttcggctgtt gaggcaattg cccgccagcc cgaagccatg ggcgatatcc gcgccaacat
      181 gatcctgact gccgctctcg tggaaggcgc tgccttcttc gcaatggtgg tcggcctgct
      241 ggttattctt accaaataac cggggcaacc gatttctccg ccgctttgcg gttggcaaag
      301 cggcggagat tttcaaccgg agttaaacag aaacagaata ccaaacacaa accttaaaac
      361 aacgacgacg catgttaatg gttcccgata caggactggc gatctggatg ctgatcacat
      421 tcctgattgt tcttttcctg ctgacgaaat tcgcgtggaa gccgatcatg aaaatgatcc
      481 gcgagcgcga agagtcgatc gaaggtgcgc tgaaatccgc tgaaaaagcg aaagaggaga
      541 tgcaggcgct gcaggccggc aacgagcgca tcctcgcaga agctcgtaat gagcgcgacc
      601 gcatgatgaa agaagcgcgc gaaataaaag acagcatcgt gaacgaggcg aaaacgaaag
      661 cgaaagaaga agccgacaag ctgctcgcct ccgcacgcga aacgatacag aacgaaaaaa
      721 tggccgccat cacggagctc aagaaccagg tggctgcact ttccatcgat attgccgaaa
      781 ggatcctgaa aagagagctc gcctccgaga acaagcagaa ggatctcatc ggtgaaatgc
      841 tgaaagaagc caagctcaac tgatcatgac ggggacacgg gtatcttacc gctacgcaaa
      901 atcgctgctc gacctttctc tcgagaaagg ccagctcgaa caggtgcatg aagacatgca
      961 gcttgtactg aataccatcc gcggcagccg ggatctcgag ctgctgctga aaagcccggt
     1021 gatcaaaacg gataagaaag atgatatcct caaggcgatc ttcggcggca agatcggcgt
     1081 gatcacgacg gcgttcatcg atattctcac gcgcaaacgc cgcgaaggtt atcttgaatc
     1141 ggtggccgac tcattcgtta accagtataa aaagcacaag aaaatcctta ccgcggtggt
     1201 caccacagct ctcggcctcg atgaaagcct gcgcgaacag gtggtgaatg tcgtgaaaca
     1261 gggcggaaaa tcggaagtgg agctgcagga aaaagtcgat gccaacatta tcggcgggtt
     1321 catcctgcgc gtgggcgaca accaggttga tgccagcatc atccgccaga tcaaaaatct
     1381 cgaaagaacc tttaacgaaa atccatacat caaagatttc tgagcgtttc agcgggatgg
     1441 attttcccat gaagatgctc ataacagata accaaagaca atggcagaag taaaacccgc
     1501 ggaagtttcc gcaatcctca ggcagcaact cgcaggcttc aagtcagcga aggaaatgga
     1561 agaagtcggc accgtgctcc aggtcggtga cggtatcgcc cgtatttacg gcctcaccaa
     1621 cgttcagtcg gtcgagctca tcgaattcga gaacggcctg aaaggcgtta cgctgaacct
     1681 ggaagaagac aacgtcggcg ccgtgctgct cggtgattcc aaaggcatca aggaaggcga
     1741 cactgtccgc cgcaccggga agatcgcctc tctcatggtg ggcgaaggaa tgctcggccg
     1801 cgtagtggat gcgctcggaa acccgatcga cggaaaaggc ccgatcaaag gtcagaccta
     1861 cgaaatgccg ctcgaacgga aagcgccggg tgttattttc cgccagccgg tgaacgagcc
     1921 gctgcagaca ggtatcaaag ctatcgacgc gatgatcccg atcggacgcg gtcagcgcga
     1981 actggtgatc ggcgaccgcc agacgggaaa aacggccgtg tgtctcgaca ccatcatcaa
     2041 ccagaaagaa ttttacgaag cgggaaaccc cgttttctgc atctatgttg cggtaggaca
     2101 gaaaggatct accgtggcca atgtcgtgcg tacgctcgaa gagaacggcg ccatgcctta
     2161 cacggttgtg gtttccgctt ccgcttccga tccggctccg ctgcagttct tcgcgcccat
     2221 ggccggcgct gccatcggtg agtttttccg cgacaccggc cgccctgcgc tgatcgttta
     2281 tgatgacctg tccaaacagg ccgtcgctta ccgcgaagtt tccctcctgc tccgccgtcc
     2341 tcccggacgt gaagcgtatc ccggcgacgt attctatctc cactcccgcc tgctcgaacg
     2401 cgcagccaag atcaatgcat cggatgaagt ggcaaagaac atgaatgacc ttcccgaaag
     2461 catccgttcc atggtgaaag gcggaggatc gctcaccgcg ctcccgatca tcgaaacaca
     2521 ggccggtgac gtttccgctt acatcccgac gaacgttatc tcgatcaccg acggacagat
     2581 cttcctcgaa tcgaaccttt tcaactccgg cgtacgtcct gcgatcaacg taggtatttc
     2641 cgtatcgcgc gtgggcggta atgcgcagat caagtcgatg aagaaagtgg ccggtacgct
     2701 taagctcgac caggcgcagt tccgcgaact ggaagcgttc gcaaagttcg gctccgacct
     2761 cgacgcagct acgaaatcgg ttatcgacaa aggatcgcgc aacgtggaga tcctcaagca
     2821 gggacagttc tcgcctttcc gcgtggaaga gcagatcgcg atcatttatt gcggaacgaa
     2881 aggtcttctg cgtgatgttc ccgtgaacaa ggtgcgcgag ttcgaaagcg atttcctcgc
     2941 ttacatgaag aacaaacatg cggacgtgct ggcagcgctc aaagccggca agctcgacga
     3001 tactatcacc gctgcgctcg aatcggcggc aaaagagatc agcgcgaaat acaaagcgta
     3061 atacaataca cacatggcga atctcaaaga ggtacgtaac cggatcacgt cggtatcatc
     3121 cacgcagcag atcacctctg cgatgaagat ggtttccgcc gcgaagcttc gccgcgcgca
     3181 ggatgcgatc acgcagatgc gcccgtacgc gaacaagctg cgcgaaattc tgggcaacct
     3241 cagcggaagc ctcgacagca gcgaaggcgt ttattcccgc aagcgggagg tgaggaatgt
     3301 gctgctcgtg gtgatcactt ccaaccgcgg tctttgcgga ggcttcaact cttccgtggt
     3361 aaaacgcgcc gtgcgcgagc tcagcgagaa ttatccttcg gcgaaagcgt cgtatttctg
     3421 catcggcaaa aaaggcggcg actttttccg gaaaactccg cacacggctg catttaccga
     3481 cgccgaaaat cccaacgcgc tgttcgacaa cctcacgttc gcgaatgtag ctcccgttgc
     3541 ggaacgcatc atgaaagcgt tcgccgacgg aacgtacgac aaggtggtgc ttatttacaa
     3601 ccagttcaag aacgctgccg tgcagatcac gcaggccgag cagttcctcc ccgttgttcc
     3661 gaacgaagga agcaaaacgc tgaagaccga ttatgttttc gaacccagcc gttcggagat
     3721 cgtgctcgat cttattccgc gctcgctgaa gacgcagctc tacaaggcgc tgctcgattc
     3781 acacgcatct gagcatggtg cacgcatgac ggccatgcac aaagcgacgg acaacgcaaa
     3841 agaactgatc cgttccctgc gcatcagcta caacaaagcg cgccaggcgg ctatcacc
//
LOCUS       DASTSF010000530         5853 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1438610,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000530 DASTSF010000000
VERSION     DASTSF010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5853
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1438610"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..229)
                     /locus_tag="VFU15_15115"
     CDS             complement(<1..229)
                     /locus_tag="VFU15_15115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719172.1"
                     /translation="MKIRLLLPLLLAFFLFSCGPKGAYNSYKDAKVHSSERIAKQQAK
                     ASKKAQKDFKKNLKKANKQGGKKGNRWSRKKK"
     gene            complement(274..1782)
                     /locus_tag="VFU15_15120"
     CDS             complement(274..1782)
                     /locus_tag="VFU15_15120"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-like domain-containing protein"
                     /protein_id="HEU4719173.1"
                     /translation="MKKTQLITLFISLLTLSAWAQTPAGKSKAPVKKQVPLDLKVKMK
                     GVTGGYCILAHHYGDKQLIQDSVKVGPDGTMEFKDTAAVPGGIYMVFTPDRKKYFEVI
                     LTDNQKFSLETDTADFVNSMKVTGNDENKFFYEYLVFLTAQQKKVQPLQDALAKARAD
                     KNADSVAMLTKKISDIDSVVKQYKRDYYQKKHPETFMAKVLHLMDEPDPLPKDPVKYP
                     NDSIYGVANYWNFRNHYWDGMDFSDDRLMYTPVYASKLKFYLDKLIPQHPDSITAACD
                     DIINRTRPSKELFKYTLIYTTYNYESSKIMGYDAIFVHLVETYYRTGQVWWVGDEQMT
                     KILNRSTQLSYSLIGKPAINLMLQDSNGVVKPLGAVNAKYTVVIFWDPTCSHCKKEVP
                     VLKHYYDSLRTAGVSVQVYAVYSELDYKGWKAYIKEHNLNWINVCGKDAQELGTAKYY
                     YDVYSTPTIYLLNDKKEILGKRFDVDGLQTFLNRQIMLDQKKTAQGNNTSPH"
     gene            complement(1857..3071)
                     /locus_tag="VFU15_15125"
     CDS             complement(1857..3071)
                     /locus_tag="VFU15_15125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719174.1"
                     /translation="MLNRLLFAHQKPALLITAVAGALVGMILLLGTLQLYFDFSDVIN
                     GNSDVTRPQYLVINKEVSMLNTFFGGQKGFSADELASLKKISGVKDIAPLTSSKFKVS
                     ISMGSGGMQGMPGVGGDLFFEAVPDNFLDVSPDAYQWKEGDSLVPLVIPRDYIKLYNF
                     GFAPAQGLPQLTEGMVGLARFTITIRGSKGDAVFHGKLAAFSERINSILAPQSFLDYA
                     NEKFEGVAPGSVAPSRVVIQCDGPATAELADYFTKNGYETSAESLRNSKLNSWLRLVM
                     GFLVGIGAVIIALSALSFLLYSLLLISKSSYEIRTLTSLGYDHVKLGRRYLEYFAVVF
                     SAILVVSLVSVRFIKLLVSHLASSKGFELPSGISAGVFVSGVLFTLVFLGLNGWAVFS
                     GIRNIASGRKSQ"
     gene            complement(3074..3715)
                     /locus_tag="VFU15_15130"
     CDS             complement(3074..3715)
                     /locus_tag="VFU15_15130"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU4719175.1"
                     /translation="MKFELNNVLPRPLSDTPLSDESCWKKRAVIGPEGNYLVEAPSGT
                     GKTTLVSIMYGMRHDYDGDVLLDGKNVRKFSISDWEALRRDLISAVFQDLRLFPQLSA
                     EENIAVKNQLTGALKEDEIRSMAETLGIGNKLGQRCGTLSLGQQQRVAIIRALAQPFR
                     MILLDEPFSHLDEENSRKAAEMISSFCERNKAALVLTSLGPNRFFEFTEKISI"
     gene            complement(3712..5292)
                     /locus_tag="VFU15_15135"
     CDS             complement(3712..5292)
                     /locus_tag="VFU15_15135"
                     /inference="COORDINATES: protein motif:HMM:NF027446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4836 family protein"
                     /protein_id="HEU4719176.1"
                     /translation="MSTSRKIIISGISLLLIGAAVFAVLKFLSGSSGAHLKVIPSDAA
                     AVLKINTTQLALKADIPSLMKLPAFSSPGSNGSPLGRMISDPMSTGLNPVADVYGFLA
                     KNGQNTVSALVFSVRDAGDLSTFIRSLSVTDVYSKEDDVWFADFNQQRCIAWTDDAGM
                     IVSTDGGDIHAYCKKLLEEGEEQSIIRNKAFASFNDKTFDAGIWLDNHALSTMNTAAS
                     ALGMMGIGEGTGEVLLSFEDEGIKATYSSDGSYDKNRLLKSGGFSDKKTEVIAPSDPV
                     VMLGLVADIAALMKSLKSDNNMNGNITGMEDLLGINDADIQKLATGEISVAFTGFRDI
                     SVYDPRVSAEVKKNIAQSKGAETAGDYALLVPMVYIDLGVTDDSLANSVLDRTGMDRT
                     GRFYAAPGINFIVYAGAKNGHLLITNDYYAEDSLVHTGTLNGKISPGWSSVTSSNSFF
                     ALADLDQSHFSPQLDSALADNFGAQELDFYFTVMKPFRDASLCSTGKGSTLTISVGPG
                     SGNSLFRLLSYYGTLAGK"
     gene            complement(5289..>5853)
                     /locus_tag="VFU15_15140"
     CDS             complement(5289..>5853)
                     /locus_tag="VFU15_15140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010526234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="C-terminal helicase domain-containing protein"
                     /protein_id="HEU4719177.1"
                     /translation="PLLMDILSQKKDLVSVIIFSSTKQNVKNMEKELKKLGLSVAAIH
                     SDLEQNEREEVLRMFRSRNIRVLVATDVLSRGIDIENISMVVNFDVPADGEDYVHRVG
                     RTARAESTGEAITFISPDDMRKFGRIEKLIGYEVEKGPVPAELGETPAYSANAPSSGK
                     RGNKGKGNSGKKRFGNNRSSHSPSKPR"
BASE COUNT         1308 a         1555 c         1566 g         1424 t
ORIGIN      
        1 gctttttctt ccggctccag cggttgccct ttttgcctcc ctgcttgttg gcctttttca
       61 ggttcttctt gaaatctttc tgcgctttct tggaagcctt cgcctgctgt ttcgcaatgc
      121 gttcgctgga atggactttc gcgtccttgt atgaattata ggcgcctttc ggtccgcatg
      181 aaaaaaggaa aaaggcgagg agcaaaggaa gcaacagccg gatcttcatc acttcttttt
      241 taaacataac gtctcacccg cccgaaaggt acgtcagtgc gggctggtat tgtttccctg
      301 cgccgttttt ttctgatcga gcatgatctg ccggttcagg aatgtctgca gcccgtccac
      361 atcgaaacgc ttgccgagga tctctttttt atcattcagc aggtagatcg taggcgtgct
      421 gtacacgtcg taataatatt ttgccgtgcc cagttcctgg gcgtcttttc cgcacacatt
      481 aatccagttg aggttgtgct ccttgatgta cgctttccac cctttgtaat cgagttcaga
      541 gtaaaccgca taaacctgca cggaaacacc cgcagtcctg agcgaatcat aataatgctt
      601 cagcaccgga acttcttttt tgcagtgact gcacgtcggg tcccagaaaa tgaccaccgt
      661 gtatttcgcg ttgacagcgc caagcggttt caccacgccg ttcgaatcct ggagcatgag
      721 attgatggcc ggtttcccga tcagcgaata gctgagctgc gtggagcggt tgaggatctt
      781 tgtcatctgc tcatcgccga cccaccagac ctggcctgtg cggtaatatg tttccaccag
      841 gtgaacgaag atcgcatcat agcccatgat cttcgacgat tcgtaattgt acgtggtgta
      901 aatgagcgtg tacttgaaca gctctttcga aggacgcgta cggttgatga tgtcatcgca
      961 ggcggccgtg atcgaatccg ggtgctgggg aatgagcttg tcaaggtaaa atttcagctt
     1021 gctcgcatac acgggcgtgt acatgaggcg gtcgtcggaa aaatccatcc cgtcccagta
     1081 atggttgcgg aaattccagt aattggccac accgtaaatg ctgtcattgg gatatttaac
     1141 cgggtccttc ggaagcggat cgggttcgtc catcaggtgc agcactttcg ccatgaaggt
     1201 ttccgggtgt ttcttctgat agtaatcgcg cttatattgt ttcaccacgc tgtcgatgtc
     1261 gctgatcttt ttcgtgagca tggcgaccga atcggcgttc ttgtccgcac gggctttcgc
     1321 cagcgcatcc tgcaggggct gcactttttt ctgctgcgct gtcaggaaaa cgaggtattc
     1381 atagaaaaac ttgttctcgt cattgccggt gaccttcatg ctgttcacga aatcagccgt
     1441 gtccgtttcc aatgaaaatt tctggttgtc ggtaaggatg acctcgaaat attttttccg
     1501 gtcaggcgtg aacaccatgt aaatcccgcc cgggactgcc gccgtgtcct tgaactccat
     1561 cgtaccgtcc ggcccgacct tcaccgaatc ctgtatcagc tgcttgtcac cgtaatggtg
     1621 cgcaaggatg caatagccgc ccgtgacgcc tttcattttc actttcaggt ccagcggaac
     1681 ctgctttttg accggggctt tgctttttcc tgccggggtt tgcgcccagg cggaaagagt
     1741 taagaggctg ataaagagag tgatgagttg tgttttcttc ataaaagagt catttgccgt
     1801 atcaaaaata agaaatcgaa agagttgaaa aacctggcag caaaaatcaa gccgtttcat
     1861 tgacttttcc tgccggatgc aatgttcctg atcccgctga aaacagccca cccgttaaga
     1921 ccgagaaaca caagcgtgaa aagtacaccg gaaacgaaaa cgcccgcgga aattccggag
     1981 ggcagctcga atcctttcga agaggcgagg tgcgacacaa ggagcttgat gaaccggacg
     2041 gagacgaggg aaacaacgag aattgcggaa aagaccaccg cgaaatattc caggtagcgc
     2101 ctgcccagtt ttacgtgatc atagccgagc gaggtaagcg tcctgatctc gtaagatgat
     2161 ttgctgatca gcagcagcga atagagcagg aaggaaagtg cgctgagcgc gatgatcacc
     2221 gcgccgatgc cgacaaggaa tcccatcacg aggcggagcc aggaattcag cttgctgttg
     2281 cgaaggcttt ccgcagatgt ttcatagccg ttcttcgtga aataatccgc aagttccgcc
     2341 gtggccggtc cgtcgcactg gatcaccacc cggctgggcg ccacgctgcc gggtgccaca
     2401 ccttcgaatt tttcattcgc ataatcgagg aagctctgcg gcgccagtat ggaattgatg
     2461 cgttcgctga atgcggccag cttcccgtga aaaacagcat cgcctttcga accgcggatg
     2521 gtgatggtga aacgcgccag cccgaccatt ccttccgtca gctgcggaag tccctgcgca
     2581 ggtgcgaaac cgaaattgta cagcttgatg taatcgcgcg ggatcacgag cggaacgagc
     2641 gaatcgcctt ctttccattg gtaagcgtca ggcgaaacat cgaggaaatt atcgggcact
     2701 gcttcaaaga aaaggtctcc gccgacgccg ggcattccct gcatgccgcc gcttcccatg
     2761 gaaatgctga ctttgaattt gctcgacgtg agcggtgcga tgtcctttac gccgcttatt
     2821 tttttcagcg aagccagttc atcggctgaa aatcctttct gaccgccgaa gaacgtgttc
     2881 agcatgctca cttccttgtt gatcacgagg tactgcggac gtgtaacatc tgaattcccg
     2941 ttaatgacgt cggaaaagtc gaaatacagc tgcagcgtcc ccagcagcag gatcatgccc
     3001 acgagcgcgc ctgcaactgc tgtgatgagc agcgcaggtt tctggtgagc gaagagcagc
     3061 cggttgagca tgatcagatg gaaatttttt ccgtgaattc aaaaaaacgg ttgggcccga
     3121 ggctggtcag cacaagagcc gctttgttcc tttcgcagaa agaagagatc atttccgccg
     3181 ccttccggga attttcctcg tcgagatggc tgaacggctc atccagcagg atcatgcgga
     3241 aaggttgggc gagcgcacgt atgatggcca cacgctgctg ctgcccgaga gaaagtgttc
     3301 cgcaccgctg gccgagcttg ttgccgatgc cgagcgtttc ggccatggac ctgatctcgt
     3361 cttctttcag cgcgccggtc agctggtttt tcacggcaat attttcttct gcgctcagct
     3421 gcgggaagag gcgcaggtcc tggaaaacgg cggaaatgag atcacggcgg agtgcttccc
     3481 aatcagaaat tgaaaatttc cggacgttct tcccgtcgag aagcacatcg ccatcgtaat
     3541 cgtggcgcat gccgtacatg atcgagacca gcgtcgtttt ccctgttccg gaaggagctt
     3601 ccacgaggta attcccttcg ggcccgataa ccgctctttt tttccagcag ctttcgtcgg
     3661 aaagcggcgt gtccgaaaga ggtcgcggaa gaacgttgtt cagctcgaat ttcatttgcc
     3721 tgccagtgtg ccgtaatagg agaggagacg gaaaaggctg ttgccgcttc ccggtccgac
     3781 gctgatcgtc agcgtggatc ctttccctgt gctgcacagc gatgcgtccc ggaaaggctt
     3841 catcaccgtg aaataaaaat ccagctcctg cgccccgaaa ttatctgcga gcgcggaatc
     3901 cagctgcggt gaaaaatgcg actggtcgag atcagcaagc gcgaaaaagc tgttcgacga
     3961 ggtgacggag ctccagccgg gggaaatttt tccgttcagc gtacccgtat gcaccagcga
     4021 atcttccgcg tagtaatcgt ttgtaatgag caggtgaccg ttcttcgcgc ccgcgtacac
     4081 gatgaaatta atgccgggcg ccgcataaaa tcgtccggtc cggtccatcc cggtccggtc
     4141 cagcacggaa ttggcgagcg aatcgtccgt gacgccgaga tcgatataca ccatcgggac
     4201 aagcagcgca tagtcgccgg cggtttcagc acctttcgac tgcgcgatat tttttttcac
     4261 ttcggcggaa acacgcggat catacaccga aatatccctg aaacccgtga acgcaacgga
     4321 aatttcacct gtggccagct tttgaatgtc cgcatcattt atgcccagga gatcttccat
     4381 cccggtgatg ttgccattca tgttgttgtc cgacttcagc gatttcatga gcgccgcgat
     4441 atccgcgaca agaccgagca ttacgaccgg atcggaagga gcaataactt ctgttttttt
     4501 gtccgaaaag ccgccggatt tcagcagccg gtttttgtca tagcttccat ccgaactgta
     4561 tgtcgctttt attccttcat cttcgaacga aagcagcact tcacctgttc cttcgccgat
     4621 tcccatcatt cccagtgcag aagcggccgt gttcatcgtg gacagcgcat gattgtcgag
     4681 ccagatgccg gcatcgaacg ttttgtcgtt gaaagaagca aacgctttgt tgcggatgat
     4741 cgactgttct tctccttcct ccaggagctt tttgcagtac gcgtgaatat cgccgccatc
     4801 cgtggaaacg atcatgcccg cgtcatcggt ccaggcgatg cagcgctgct ggttgaaatc
     4861 agcgaaccag acatcgtcct cttttgaata aacatccgtg acggacaacg atctgatgaa
     4921 cgtggaaaga tctccggcgt cgcgcacgct gaagacaaga gcggaaaccg tgttctgccc
     4981 gttcttcgcg aggaagccgt acacatccgc aaccgggttc agcccggtgg acatcggatc
     5041 agagatcatc cggccgagcg gactgccgtt gctgcccgga gaactgaacg caggcagctt
     5101 catcagcgag ggaatatccg ctttaagcgc cagctgcgtg gtgtttattt tcaggacagc
     5161 cgctgcgtcg gaaggaatga ctttcaggtg ggcgccggaa gaccccgaaa ggaatttcag
     5221 caccgcaaaa acagcagcgc caatgagaag aagggaaatt ccggagatga ttattttccg
     5281 tgaagtgctc atcggggttt tgaaggagaa tgcgaagacc tgttgttccc gaaacgtttc
     5341 ttcccggaat tgcctttgcc tttattgcca cgcttgccgg aggaaggcgc attcgctgaa
     5401 taagccggcg tttcaccgag ctcggcaggt acagggcctt tttccacttc gtagccgatc
     5461 agcttttcga tccggccgaa cttgcgcata tcgtcgggag aaatgaacgt gatcgcttcg
     5521 ccggtggatt ccgcacgcgc ggtacgtccc acgcggtgga cgtaatcttc gccgtcggcg
     5581 ggaacatcga aattcacgac cattgaaatg ttctcgatat cgatgccgcg ggaaagcacg
     5641 tcggtggcga cgagcacgcg gatattcctg ctcctgaaca tgcgcagcac ttcctcgcgt
     5701 tcgttctgct cgagatcgga atggatggcc gccacggaaa gacccagctt cttgagctct
     5761 ttctccatgt tcttcacgtt ctgtttcgtg gaggagaaaa tgatcacgct gacgagatct
     5821 ttcttttgcg aaagaatgtc catcagcagc ggg
//
LOCUS       DASTSF010000531         5343 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_990584,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000531 DASTSF010000000
VERSION     DASTSF010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5343
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_990584"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..600
                     /locus_tag="VFU15_15145"
     CDS             <1..600
                     /locus_tag="VFU15_15145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719178.1"
                     /translation="TIDRIKPKINKLYNDFEKREFDRKIESFLVYLLKNSRSKFGRTK
                     KLKDRKVHDVEVFLPDNIREKDLLIDRSRLLTIDYMQLLEPPPVQVANPEFDRDDLAL
                     QAEVRRRQIERDKRVEQWFTKISEELKKKKKVNFGDYYYQIMQKDNDYEAAIKEATLV
                     LKEFSKMEGVEVTVREEFVLDPKQPNIGIWKMQLKNTAS"
     gene            567..1268
                     /locus_tag="VFU15_15150"
     CDS             567..1268
                     /locus_tag="VFU15_15150"
                     /inference="COORDINATES: protein motif:HMM:NF016198.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HEU4719179.1"
                     /translation="MEDATKKYGFLETDEAENLFADLDFSLKSGQHIQNYPHQVELYN
                     FIEKYEDEIRNYYRSLFRVNLASKGTDYNRYYYLEIDDESRGIVKTRAKKLSPEYTLL
                     GILLLKIHRIDKYFINDIYVPDLIQLIKSNEEYKNYIYNLFAKRREKEATEIDENTID
                     EWVRNALQQFERLGWIHYDPRNKDLFEIMPSIDRLFTMYSYEIHNIDRLIDETNTMSE
                     TTGGLYPVEEGGDND"
     gene            1325..4810
                     /locus_tag="VFU15_15155"
     CDS             1325..4810
                     /locus_tag="VFU15_15155"
                     /inference="COORDINATES: protein motif:HMM:NF016217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719180.1"
                     /translation="MKQYPRIYSLSTVGVIYHNNCDYLLHPLRTDFTGGSGSGKSVIA
                     DLLQLIFVAKKEFWESGTDVMTTEKRTVEGIVLKQGGEQGNLAYAFANIEIDKGKYIV
                     VGTHVQTNSRSIYPFIVQKGFDFGSESENNLDPMDKFLLYRDFLVNNQILSIDKMKEV
                     LEKRGLNLRHFNNRTSEFHRVLFNNQILSLDLSHDEKKLKTFAQIIQSFSRGKGIGAK
                     SEDLKNFLFSNDDDQNKEYEKRKKEIEEAHEEYKRRSRVYETVKLKKDRLLALLEDKK
                     KRDKALHRALFLETGWAFQQKDVVGRDLIRTQKETAYVNLQQIMLEQRVNELNSDAVE
                     RELDSIVGERERLLAELESKKKQREFAEKNRASYDKAYSIAQSEYDEASAKWSRVKSV
                     EGWLRMYKTVDAVREQFTKQDKVARDKQRLEEFENFLSGHRIYAEFAESTWAQDHRLA
                     QAEYDGLMDQLDAEIFRLEQVLPLFDGENKDSVVQWAIKNAGKLSKEEESVLMHFKAL
                     STKKPEGKEGVRFVPDPESIYRNVKPVDKDANGFWVDFGGVMEYVPFVSRQLFNNPKT
                     IEQELKKYGDTIGKELEKLTAERDKHRRLRELLVEAGFNQQLSEIFLRREELVKFVPD
                     ETMLMTKEDFESTMDLFTNPQNNMRLQYEEAKKNWEDALQKLREADDIISNSQGGLIS
                     LEGQIRQLDLTQMPDKERELKNFRQERKALEERFSDWQKDVTTYLRASEGEQSQAKVN
                     QEIRAIEKEHGSSTKMLYNAFTQRLQEISRKQGSLEQRLIGLMEQMPSLDTRFNEAHK
                     KYTEVIGEFRPDELTEQVEESQVNKAKVTVITTQKAYESNFEAILKDDKFNIDDERLK
                     GDHKYDFQTLAEVMLPKIIQDPAKVEEDLSNDVEEHLRIINQKMNEINDHKLKIINGI
                     FSKIEEVYSEYRDRIDDMREFFRQNKISGGHYAEINFEPSKGYPINWIRSLNKNIAAK
                     AQNVGLFKMFETENEGADAIILKAFLEHSDGKNVKPDIRKLTNPKSYFDLSISLKTPK
                     GEDAPGSNGQNYTMLALLCIARLSIIDSDRLSGKDRKIKRGVRFMTIDEVAGLGENFD
                     MLYELAKNYDYQILTMTIDPIGRFEEGKQNIYTLRKNNNPEFHNVNPTPLAQFSYDNI
                     VEHLEDYISSLQVQN"
BASE COUNT         1532 a         1405 c         1401 g         1005 t
ORIGIN      
        1 acgattgacc gcatcaagcc gaagatcaac aagctctaca acgatttcga aaagcgcgag
       61 ttcgaccgca agatagaatc gttcctcgtt tacctgctga aaaattcgcg cagcaaattc
      121 ggccgcacga aaaaactcaa ggaccgcaaa gtgcacgacg tggaagtttt cctcccggac
      181 aatatccgcg agaaagacct gctcatcgac cgctcgcgcc tgctcacgat cgactacatg
      241 cagctgctgg aaccgccgcc ggtgcaggtc gccaatcctg aattcgatcg cgacgacctt
      301 gcgctccagg ccgaggtgcg ccgccggcag atcgagcgcg acaagcgcgt cgaacagtgg
      361 ttcaccaaaa tttcggaaga gctgaagaag aaaaagaaag tcaatttcgg cgattactac
      421 taccagatca tgcagaagga caacgactac gaagccgcga tcaaggaagc gacgctcgtg
      481 ctcaaggaat tctcgaaaat ggaaggcgtg gaagtcaccg tgcgtgaaga atttgtactg
      541 gatcccaaac aacccaacat cggaatatgg aagatgcaac taaaaaatac ggcttcctag
      601 aaacggacga agccgaaaat cttttcgctg atctcgactt ctcgctgaaa agcgggcagc
      661 atatacagaa ctacccgcac caggtggagc tgtacaactt catcgaaaag tatgaagacg
      721 agatccggaa ttattaccgc agccttttcc gggtgaacct cgcttcgaaa ggaacggatt
      781 ataaccgcta ttactatctc gagatcgacg acgaaagccg cggcatcgtg aagacccgcg
      841 cgaaaaagct gtcgccggaa tacaccctgc tggggatcct cctgctgaag atccaccgca
      901 tcgacaagta tttcatcaac gacatctacg ttcccgatct catccagctc atcaaaagca
      961 acgaagagta caagaactac atctacaacc tcttcgcgaa acgccgtgaa aaagaagcga
     1021 cggagatcga cgagaacacg atcgacgaat gggtgcgcaa cgcgctgcag cagttcgaac
     1081 gcctcgggtg gatccattac gatccgcgca acaaggacct tttcgagatc atgccgtcca
     1141 tcgatcgcct gttcacgatg tactcgtacg aaattcacaa catcgaccgc ctcatcgatg
     1201 aaaccaacac gatgtcggaa accaccggcg gactttatcc cgtcgaagaa ggcggagaca
     1261 acgactgacc cctgcatccg ttaagcttcg ccattcagcc tgacaaaacg atccaacaga
     1321 aatcatgaaa caatatcctc gcatctattc gctgtcgaca gtcggagtga tctatcacaa
     1381 caactgcgac tacctgcttc acccgctgcg taccgacttt accggcggat cgggttccgg
     1441 gaaatcggtg atcgccgatc ttctccagct gatcttcgtg gcgaaaaagg aattctggga
     1501 atccgggacg gacgtcatga cgacggaaaa acgtaccgtg gagggcatcg tcctcaagca
     1561 gggcggtgaa cagggaaacc tggcgtacgc atttgcgaac atcgagatcg ataaaggaaa
     1621 atacatcgtc gtcggtacgc atgtgcagac gaactcccgc tcgatctacc cgttcatcgt
     1681 gcagaaagga ttcgacttcg gttcggaaag cgagaacaac ctcgacccga tggacaagtt
     1741 cctgctgtac cgcgatttcc tcgtgaacaa ccagatcctt tccatcgaca agatgaagga
     1801 agtgctggaa aaacgcgggc tcaaccttcg tcatttcaac aaccgcacgt cggaatttca
     1861 ccgtgtgctg ttcaacaacc agatcctttc cctcgatctc agccacgacg agaaaaagct
     1921 gaaaacattc gcgcagatca tccagtcgtt ctcgcgcgga aaaggtatcg gtgcgaaatc
     1981 ggaagacctg aagaacttcc ttttctccaa tgatgacgac cagaacaagg aatacgaaaa
     2041 acgcaagaag gagatcgagg aggcgcacga agaatacaag cgccgttcac gcgtatacga
     2101 aacggtgaag ctgaaaaaag accgactgct cgcactgctc gaagacaaaa agaaacgcga
     2161 caaagcgctg caccgtgcgc tgttcctcga aaccggctgg gcgttccagc agaaggacgt
     2221 cgtgggccgc gaccttatcc gcacgcagaa agaaacggct tacgtgaacc tgcagcagat
     2281 catgctcgag cagcgggtga acgagctgaa ttcggacgcc gtggaacggg agctggattc
     2341 cattgtcggt gagcgcgaac gtttgctcgc cgagttggaa tcgaagaaga agcagcgcga
     2401 attcgccgag aagaaccgcg cgtcgtatga caaagcgtat tcgatcgcgc aatccgaata
     2461 cgacgaggcc agcgcgaaat ggtcgcgtgt gaaatcggtg gaaggctggc tgcgcatgta
     2521 caaaacggtg gacgccgtgc gtgagcagtt cacaaagcag gataaagtgg cgcgcgacaa
     2581 gcagcgtctc gaggagttcg agaatttcct ttcgggccac cgcatttacg cggaatttgc
     2641 cgaatcgaca tgggcgcagg atcatcgcct tgcgcaggcc gaatatgacg ggttaatgga
     2701 ccagctcgat gcggaaattt tcaggctcga gcaggtattg ccgctgttcg acggcgagaa
     2761 caaagattct gttgtccagt gggcgatcaa gaatgctgga aaactttcaa aagaggaaga
     2821 atccgtgctg atgcatttca aggcgcttag cacgaaaaag ccggaaggaa aagaaggcgt
     2881 tcgtttcgtt cccgatcctg aaagcattta caggaacgtc aagccggtcg ataaagacgc
     2941 aaatggcttc tgggtggatt tcggcggagt gatggaatat gttcccttcg tttcacggca
     3001 gctgttcaac aacccgaaaa cgatcgagca ggagctgaaa aaatacggcg acacgatcgg
     3061 caaagagctg gaaaagctaa ccgcggaacg cgacaagcac cgccgtctcc gcgagctgct
     3121 cgtggaagcg ggattcaacc agcagctttc tgaaattttc cttcgccgcg aagagctcgt
     3181 gaaattcgtt ccggacgaaa cgatgctgat gacgaaagag gattttgaga gcacgatgga
     3241 tcttttcacg aacccgcaga acaacatgcg cctacagtac gaagaggcga aaaagaactg
     3301 ggaagatgcg ctgcagaaat tacgcgaagc cgacgacatc atctcgaact cgcagggcgg
     3361 acttatttcc ctcgaaggac agatccgcca gctcgatctt acgcagatgc cggacaagga
     3421 acgcgagctg aagaatttcc gccaggaacg caaagcgctc gaagagcggt tcagcgactg
     3481 gcagaaagat gtgacgacat atctccgcgc ttccgaaggc gagcagagcc aggcgaaagt
     3541 gaaccaggag atccgcgcga tcgaaaaaga gcacggttcg agcacgaaga tgctgtacaa
     3601 cgcgttcacg cagcgcctgc aggaaatttc ccgcaaacag ggttcgctcg agcagcgcct
     3661 catcggcctg atggagcaga tgccttcgct cgatacgcgt ttcaacgaag cgcacaaaaa
     3721 atatacggag gttattggcg agttcaggcc ggacgaactg accgaacagg tggaagaatc
     3781 gcaggtgaac aaggcgaaag tcaccgtgat caccacgcag aaagcgtacg agtcgaactt
     3841 cgaagccatc ctcaaagacg acaagttcaa catcgatgac gagcgcctga aaggtgatca
     3901 caaatacgat ttccagacgc tggccgaagt gatgctgccg aagatcatcc aggatcccgc
     3961 gaaagtggaa gaagaccttt cgaacgacgt ggaagagcac ctgcgcatca tcaaccagaa
     4021 gatgaacgag atcaacgacc acaagctgaa gatcatcaac ggcatcttct cgaagatcga
     4081 ggaagtgtac agcgagtacc gcgaccgcat cgacgacatg cgcgagtttt tccgccagaa
     4141 caaaatttcg ggcggtcact acgccgagat caatttcgaa ccgtcgaaag gctacccgat
     4201 caactggatc cgcagcctca acaagaacat cgcggcgaaa gcgcagaatg tgggcctttt
     4261 caaaatgttc gaaacggaga acgaaggcgc cgacgcgatc atcctcaagg cgttcctcga
     4321 gcacagcgac ggcaagaacg tgaagcccga catccgcaag ctcacgaacc cgaaatcgta
     4381 cttcgacctt tccatttcac tgaaaacacc gaaaggcgag gacgcgccgg ggtcgaacgg
     4441 gcagaactac acgatgctcg cgctgctctg tatcgcacgt ctttccatca tcgacagcga
     4501 ccgcctcagc ggaaaggacc gcaagatcaa acgcggcgtg cgcttcatga cgatcgacga
     4561 agtcgcgggt ctcggggaga acttcgacat gctgtacgag ctggcgaaga attacgatta
     4621 ccagatcctt acgatgacga tcgacccgat cggcaggttc gaggaaggaa agcagaacat
     4681 ctacacgctg cgcaagaaca acaaccccga gttccacaac gtgaacccga cgccgctggc
     4741 gcagttcagc tacgacaata tcgtggagca cctcgaagat tacatcagct cgctgcaggt
     4801 gcagaattga taagcggggt tataatttgc aaaacgaaac gccggacaat cgtgttccgg
     4861 cgttttttgt ctgaactttt ttacaaaaac ccggcagtaa attctttcgc gtcctggcga
     4921 ggattcttct ttttttaacc acaaagcgca ccaagtgttt tcacaaagcg cacggaggaa
     4981 gttctccgag atcttcgtgc agagctttgt gttctccgtg gtgaagcgat gaaaatattt
     5041 tacgtcctgg cgagaattct ttttttaacc acaaagcgca ccaagtgttt tcacaaagca
     5101 cacggaggaa gttctccgag accttcgtgc agagctttgt gttctccgtg gtaaaagcga
     5161 tgaaaatatt ttgcgtcctg gcgaggattc ttttttaacc acaaagcgca ccaagtgttt
     5221 tcacaaagcg cacggaggaa gctcttcgtg gtgaagcgat gaaaatattt tacgtcctgg
     5281 cgagaattct ttttttaacc acaaagcgca ccaagtgttt tcacaaagca cacggaggaa
     5341 gtt
//
LOCUS       DASTSF010000532         7701 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1442190,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000532 DASTSF010000000
VERSION     DASTSF010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 7701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7701
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1442190"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..699)
                     /locus_tag="VFU15_15160"
     CDS             complement(<1..699)
                     /locus_tag="VFU15_15160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719181.1"
                     /translation="MNNPPASGGSSRLPRLIVTSVFALAIVLARYFSGHTAQAEPMLI
                     IGKGSSYEKEWNRVDSLADKGLYRSALDLTNQIYDRAKKENNSGQVVKALIHRFKFSQ
                     QFQENSEDFAIYDLRNELKTAKFPLSPVLHSMLGDLYWQYYQRNSWKFSQRTQTSGFQ
                     NDSINTWDLNHLVSEAIKNYNASIVPVDSLFRTDEGIYADVLTKGNAEKDLRPTLYDF
                     LAQRAVAFFTAASPA"
     gene            complement(944..1855)
                     /locus_tag="VFU15_15165"
     CDS             complement(944..1855)
                     /locus_tag="VFU15_15165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719182.1"
                     /translation="MIRTICIIAVFLAACTGSHQPGQWNKKDLLKHFGFDCARASESS
                     RECDTSCYDFHFVQLAKSDTEYLFLRGEFCSGSGYGHPDAYLCMVSPQQKIIRRFNAV
                     DSIFVYGSSTAGHRDIGVTKRLYFSGHPNGSAEVRYEWDGGKYQPRTIERVNGIKKDL
                     DILCLFLIGNEICGWEQLDSSLHPGSSTDSFYPEYFQEYRFPDNKKNDSGKIILSYHD
                     TDLFRCQTWIIHYEDGKYALVQSFRDQLLSPAGEYGADSLYIRYNSPMDSLENAGALY
                     LLKDGAYIPSAPVKDPRFYGDDFDESK"
     gene            complement(1852..3462)
                     /locus_tag="VFU15_15170"
     CDS             complement(1852..3462)
                     /locus_tag="VFU15_15170"
                     /inference="COORDINATES: protein motif:HMM:NF025078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HEU4719183.1"
                     /translation="MKTKKFVRGKMSCFSEVYNGKHKTGLMYEHGYNEDSIHLHRKMR
                     EQEEEVRRRSDIKKDEKPSALTVSKEDDNSEKEAESIAQKVSAGQKPNFSITAPPSGS
                     VQAKGAAIEPDEGFQSLLGGSKGDGRPMDDSVMAEMQTKMGTDFSGVKIHTGMNAHDM
                     SESINAKAFTHGQDIYFRNGNYNPQTTEGKALLAHELVHTRQEKVGEGNSIIHRQRDQ
                     LTEVKAVKSEVVDLAASGVKGQWTQVKNRFKDRNKAVSDGNAIKEWRKGNEAAMDNLM
                     GTLRWASLKADLDNAKEDGTEVKLPADNYESLLTAEQADADALKGNLAKGTKSAVISF
                     INVLRKYVSLRASLSSKEQFFHQYDSYFVNPDVIKILNAETPAGLFTSAQLKAECGAE
                     SLDLTGPATAGNYIGIAGLGPDAQTAGISWAKSKAGVNIPTTIKSGNSMIDARLDPQT
                     AIETQAAVLGSVLDEVTKLLGTRMPKDDMEKRKLVWARYNTRRQTFVDAVNAAETKLG
                     TTDPVTFDLLTTLPPATQKYIPAIIKRL"
     gene            complement(3570..4655)
                     /locus_tag="VFU15_15175"
     CDS             complement(3570..4655)
                     /locus_tag="VFU15_15175"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU4719184.1"
                     /translation="MKIPATIFARMQQVDYLITGQGISGSVLAMELLSRGRSVAIIDS
                     PEFSRSSHVAAGAFNPFNFRTMQNNWKADALAPLVRDFYRKCEQTLGAEFFHERKIFK
                     ILTGENEKRQWENACREKPGLFADPLIRENPFASVIDAPYGMGVVNEAGNISTGIFLD
                     SVKENVRAAGNYFTEKFEFGLLHVSENEIVYADTIRAKHIVFCMGFPGKDNPFFPSLP
                     LRAVKGQTIHVHIPGLNCGEIINRGVYLLPLEKEYFVCGATFENDRADEIPDEAGKQF
                     LLSRLENFIRVPLRVESVFAGVRPAVKDRRPLAGTAQGKKNVAVFNGMGSKAVFLSPW
                     LAKCLADHFEKGDALPVEVDVNRFVTR"
     gene            complement(4652..4828)
                     /locus_tag="VFU15_15180"
     CDS             complement(4652..4828)
                     /locus_tag="VFU15_15180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719185.1"
                     /translation="MKKQVRKNLLRFSKRGFPVQPLKALYEEEVKTDTVSKNSRENEA
                     AKAAEKDEARKEPP"
     gene            complement(4825..5871)
                     /locus_tag="VFU15_15185"
     CDS             complement(4825..5871)
                     /locus_tag="VFU15_15185"
                     /inference="COORDINATES: protein motif:HMM:NF012991.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-deoxy-7-phosphoheptulonate
                     synthase/chorismate mutase type II"
                     /protein_id="HEU4719186.1"
                     /translation="MKNKTEKILIAGPCSAESAEQMLQSAAMLSLISSHGILRAGCWK
                     PRTSPGGFSGYGEEALRWLKDAADAYALPWITEAGTAAHAEKILRAGGTQLWIGARTT
                     GDPFAVSEIAIAMEGTGAGVWIKNPPQPDVKLWAGAAERFLRRAVPVHGFIHRGYKTW
                     PQTEWRNAPHWNVQEELRTIFPGARIISDPSHMTGQSGRVAAAGLTALQLGADGLMVE
                     MHPHPENAMTDRGQQLSPGELLELKCAMEKTGKAGEIGKLRTQIDELDEMLWAILGER
                     MRMAEKIGAQKHSVGMEVLQEKRRAEVKSSFRKNGHRCGLTPAESGALFEWLHAVSVR
                     KQNDVTRIPEKSHA"
     gene            complement(5868..6662)
                     /locus_tag="VFU15_15190"
     CDS             complement(5868..6662)
                     /locus_tag="VFU15_15190"
                     /inference="COORDINATES: protein motif:HMM:NF012998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase domain-containing
                     protein"
                     /protein_id="HEU4719187.1"
                     /translation="MRPLRISIPARENSFHHHAARRYFGEDAVMLHTGDMRSAIARAE
                     ENLSDYTLLACSNTVAGHFYETYRLIYESKLHILDEVEWPVSLCLAAKPGVSLAEIRT
                     AASHPYALMQAEKTLLRKKITGRKNTADTVEAARIVSRNSAGTLACVCAADVAERSGL
                     QILLENVQEHRNNRTRFFIAGKGEGLHDHPDKLIVAADAERLERPFHNAISVHRVFPA
                     KQKDIPVFAELVREDGWKMIPGGLHVLGKICSAGKRTNHKPIHISL"
     gene            complement(6797..>7701)
                     /gene="asnA"
                     /locus_tag="VFU15_15195"
     CDS             complement(6797..>7701)
                     /gene="asnA"
                     /locus_tag="VFU15_15195"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013064292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HEU4719188.1"
                     /translation="PIAVFRGTGENDDLNGVERPVAFALTAQRDGVAEVVHSLAKWKR
                     RKLASLGCPPGEGIITDMHALRPDEELSNIHSAYVDQWDWEKVICRKDRSLVFLYETV
                     QDIYSCIRETQELLSEKYSALPAFLPPHIHVVHAERLRELHPELSPKEREDKVCREKG
                     AVFIIGIGGKLKDGKEHDLRAPDYDDWSTATSEDRCGLNGDILVWNPELGRAFEISSM
                     GIRVDREALLHQLRLLGLERRLELPWHRSLVEGKLPLTIGGGIGQSRLCMLLLRKKHI
                     GEVQPGLWPDTVIEQCKAKNIPLL"
BASE COUNT         1664 a         2117 c         1900 g         2020 t
ORIGIN      
        1 ggccggtgaa gctgcagtga aaaaagcaac ggcccgctgc gcgaggaaat catacagcgt
       61 ggggcggagg tctttttccg cgttgccttt cgtgagcaca tcggcgtaaa tgccttcgtc
      121 ggtgcggaag aggctgtcga ccgggacgat cgatgcgttg tagtttttga tcgcttcact
      181 gacgaggtga ttgaggtccc acgtgttgat gctgtcgttc tggaatccgg acgtctgtgt
      241 gcgttgcgaa aacttccagc tgttgcgctg gtaatattgc cagtaaagat cgccgagcat
      301 ggaatgcagc acggggctga gcgggaattt cgccgttttc agctcgttgc gcaggtcgta
      361 aatagcaaaa tcttcgctgt tctcctggaa ctgctgcgag aatttgaacc ggtgaatgag
      421 cgccttgacg acctgcccgc tgttgttttc ttttttggcc cggtcgtaaa tctggttcgt
      481 caggtcgagc gcggaacggt aaagtccctt gtcggcgagc gaatccacgc ggttccattc
      541 tttttcgtag gagctgccct tgccgatgat gagcatgggt tcggcctggg cggtatgacc
      601 ggaaaagtag cgggccagca cgatggccaa tgcaaaaacg ctggtgacga tcaggcgggg
      661 aaggcgtgaa gaaccgccgg aagcaggagg attattcata agattcagct gatgtttgaa
      721 taccagatgc aagttagccc gaaattccac agacgaacgg cgcaattaat accggggcag
      781 gacgaattgg caaatggctg tttagctgaa tctgtttagg gagttattcc gaaatggcgg
      841 atcaacaata atcaggctac agtcataaga gaacttgtca aaatgacaaa aaactactta
      901 tctccggtcg atcgcaaagc ggagcaaggc gaaaaattgc aagctatttg gattcatcaa
      961 aatcatcccc gtaaaacctt gggtctttta caggtgcaga agggatgtat gcgccatctt
     1021 tcaacaggta aagagcacca gcgttttcca gtgagtccat cggactgtta tacctgatgt
     1081 aaagagaatc cgctccatat tctcccgcag gtgaaagcag ttggtctctg aacgactgaa
     1141 caagcgcgta tttgccgtct tcataatgaa tgatccaagt ctggcagcga aacaaatccg
     1201 tgtcatgata actcaaaatg atctttcccg agtcattttt tttattgtcc ggaaagcggt
     1261 attcctgaaa atattcagga tagaaactgt ctgttgatga accaggatgg agcgagctgt
     1321 ccaattgctc ccatccgcat atttcattcc cgatcaaaaa caggcacaga atgtcgaggt
     1381 ctttttttat gccgttaacc cgctctattg tccggggctg atatttcccg ccgtcccatt
     1441 catacctcac ctctgcgcta ccgttgggat ggccggaaaa atacaacctc tttgtcacgc
     1501 ctatatcccg gtgcccggct gtagacgagc catacacgaa aatgctgtcc accgcattga
     1561 atcgcctgat aattttttgc tggggagaca ccatgcagag ataggcatcc gggtgaccat
     1621 aaccgcttcc gctgcagaat tcacccctaa ggaaaagata ttccgtatcg ctttttgcaa
     1681 gctgcacaaa atggaaatcg taacaggatg tatcgcattc cctcgagctt tcgcttgccc
     1741 gagcgcagtc aaatccaaaa tgctttagca gatctttttt attccattga cccggctggt
     1801 ggctcccggt acatgcggcg aggaaaacag caataatgca gatcgtgcga atcatagtct
     1861 ttttataata gcaggaatat atttttgggt agcagggggt aatgtcgtta aaaggtcgaa
     1921 agtaacagga tctgtagtgc caagttttgt ttctgccgca ttaaccgcat ccacaaatgt
     1981 ttgtctcctg gtgttataac gcgcccaaac tagttttctt ttttccatat catccttcgg
     2041 cattcttgtg ccaagaagtt ttgttacttc atctaacacg gatcctaaaa ctgctgcctg
     2101 ggtttcaatt gcagtttgtg gatcaagtct cgcgtcaatc attgaatttc cgcttttaat
     2161 agttgtggga atattgaccc ctgctttgct ttttgcccac gatataccgg cagtctgggc
     2221 gtccggaccc aatcctgcta ttccaatgta atttccggca gtggccggtc ctgtcagatc
     2281 caaagactcc gcaccacatt ctgccttaag ctgggccgag gtaaacaacc ctgccggggt
     2341 ttcggcgttg agaatcttaa tcacatctgg atttacaaaa tacgaatcat actgatgaaa
     2401 aaactgctct ttggaagaaa gagaggctct gagtgaaacg tacttcctta aaacattgat
     2461 aaacgatatt acggcactct tagtgccctt tgcaagatta cctttaagcg catccgcatc
     2521 cgcctgttcg gctgtcaata gcgattcata attatctgca ggcaatttaa cctccgttcc
     2581 atcctctttt gcattatcca ggtctgcctt taacgacgcc catcttaggg tccccatgag
     2641 attatccatt gccgcttcat ttccttttct ccattcctta attgcatttc catcactaac
     2701 agctttattt ctgtccttga atctgttttt aacctgcgtc cattgacctt tgacgccact
     2761 tgcagctaaa tccaccactt cgctttttac tgctttcact tctgtcaact gatctctttg
     2821 cctatgtatg attgaattgc cctcgccgac tttctcctgc ctcgtatgca ccagctcgtg
     2881 cgccagcagc gcttttccct ccgtcgtctg cgggttatag ttcccattcc tgaaataaat
     2941 gtcctgaccg tgtgtgaatg ccttcgcgtt gatgctctcg ctcatatcat gggcattcat
     3001 gcccgtgtgg atcttcaccc cgctgaaatc cgttcccatt ttcgtctgca tttccgccat
     3061 cacagaatca tccatgggcc gcccgtcacc tttggaacca ccgagcaatg actgaaagcc
     3121 ttcatcgggt tcaattgccg ctcctttcgc ctgcacagaa ccagatggcg gagctgttat
     3181 actgaaattc ggtttttgcc cggcagacac tttttgcgca atggactccg cttccttttc
     3241 ggaattgtcg tcttccttgc tgaccgttag cgctgatggt ttctcatctt tttttatgtc
     3301 cgaccggcga cgcacctctt cttcctgctc acgcattttg cggtgaaggt ggattgaatc
     3361 ttcattatac ccgtgttcat acatgagccc ggttttatgt tttccattat aaacttccga
     3421 aaaacaggac atttttccgc ggacaaattt tttcgttttc aagtcctgaa aattcgcgaa
     3481 gatccattca attctctcac tttcagcaaa aaagacgtgc ggacacctcc tgttttttca
     3541 cacgttcctg caaaatatac cccggaaatt caacgggtaa caaaacggtt cacgtccact
     3601 tccacgggca atgcatctcc tttttcgaaa tgatccgcga ggcatttcgc cagccagggc
     3661 gaaagaaaaa cagctttgct tcccatgccg ttgaacacgg cgacgttctt ttttccctgc
     3721 gcggttcctg cgaggggacg gcgatccttt acggctgggc ggacaccggc gaaaaccgat
     3781 tccacacgca acggtacgcg gataaaattt tcaaggcgcg acaggagaaa ctgctttccc
     3841 gcttcatcgg gaatttcatc ggcgcggtcg ttctcgaaag tggcgccgca cacgaaatat
     3901 tctttttcaa gcggcaggag ataaactccc cggttgatga tctcgccgca attcagcccg
     3961 ggaatatgca catggatggt ctgtcctttc actgcacgca acggcagcga cgggaagaac
     4021 gggttgtctt tcccgggaaa tcccatgcag aaaacaatgt gttttgcgcg aatagtatcc
     4081 gcgtatacta tttcattttc cgaaacatgc agcagcccga attcgaattt ttcagtgaaa
     4141 taatttcctg ctgcacgcac gttttctttt acgctgtcaa gaaaaattcc cgtgctgatg
     4201 ttgcccgctt cattcaccac acccatcccg taaggcgcat cgatgaccga agcaaacgga
     4261 ttctcccgga tcagcggatc ggcgaacagc ccgggctttt cacggcaggc gttttcccac
     4321 tggcgttttt cattttcacc ggtaagtatc ttgaagatct tccgttcatg aaaaaattcc
     4381 gcaccgagcg tttgttcgca tttccggtaa aaatcacgga caagcggtgc aagcgcatcc
     4441 gctttccagt tgttctgcat cgtccggaaa ttgaacgggt tgaatgcacc ggcggccacg
     4501 tgcgagctcc gggaaaattc cggtgagtcg atgatcgcaa cggatctccc gcgcgaaagc
     4561 agctccatgg caagaacgct gccggaaatt ccctgccccg tgatgaggta atcaacctgc
     4621 tgcatgcgcg caaagatagt cgcaggaatt ttcacggcgg ctcttttcgc gcttcgtctt
     4681 tttccgctgc tttggcggct tcattttccc tgctgttttt cgaaaccgtg tctgtcttga
     4741 cctcttcttc gtaaagagcc ttcagcggct gaacgggaaa tccgcgtttg gaaaaacgca
     4801 gcaggttctt ccggacctgt tttttcatgc gtgacttttt tcgggaatgc gtgtgacatc
     4861 attttgcttc cgcacggaaa ctgcgtgcag ccattcgaac agggcacccg attcggctgg
     4921 tgtaaggccg caccggtgac cgtttttccg gaaactgctt ttcacttccg ccctgcgttt
     4981 ttcctgcagc acctccatcc cgaccgaatg cttctgcgca ccgatctttt ccgccatgcg
     5041 catccgttcg cccaggatcg cccacagcat ttcgtccagc tcgtcgatct gcgtgcgaag
     5101 cttcccgatc tcccctgctt tccccgtttt ttccattgca catttcagct ccagcagttc
     5161 gcccggcgat aattgctgcc cgcggtcagt cattgcattt tccgggtgcg gatgcatttc
     5221 caccatcagc ccgtctgcgc cgagctgcag cgctgtcagt ccggccgcag ccactcgccc
     5281 gctttgcccg gtcatgtggc ttggatcgga aataatgcgc gcgccgggaa aaatcgttcg
     5341 caattcttcc tgcacattcc agtgcggcgc gttgcgccat tccgtctgcg gccacgtttt
     5401 gtacccgcgg tgaatgaagc cgtgcacggg gacagctctc cggagaaaac gttctgcagc
     5461 gccggcccac agcttcacat ccggctgcgg aggatttttg atccacacac ctgcgccggt
     5521 tccttccatc gcgatggcaa tttcagagac agcgaacgga tcgcctgtcg tgcgcgcacc
     5581 gatccacaat tgcgttccgc ccgcgcggag gatcttctcg gcatgcgccg ctgttcctgc
     5641 ttccgtgatc cagggaagcg cataagcatc cgcggcgtct ttcagccagc gcagggcttc
     5701 ttcgccgtag ccggaaaatc cgcccggcga tgtgcgcggt ttccagcagc cggcgcgaag
     5761 gattccgtgc gatgaaatga gtgaaagcat cgctgccgat tgcagcatct gttccgcgga
     5821 ctctgcgctg cacggacctg cgatgagaat tttttctgtc ttgtttttca taatgaaatg
     5881 tgaataggtt tatgattggt tcttttcccc gccgagcata ttttcccgag cacgtgcaac
     5941 ccgccgggaa tcattttcca cccgtcttcc cgcaccagct cggcgaacac aggaatgtct
     6001 ttttgcttcg cgggaaaaac ccggtgaaca gagattgcat tgtgaaaggg ccgttcgagc
     6061 cgttctgcat ccgccgcgac gatcagctta tcgggatgat cgtgcaatcc ttctcctttt
     6121 ccggcaatga aaaaccgcgt ccggttattc ctgtgttcct gcacattttc aagcaggatc
     6181 tgcagaccgg atctttccgc cacatccgct gcgcatacac acgcaagcgt accggccgaa
     6241 ttcctgctga cgatcctcgc ggcttcaacg gtatctgccg tgtttttccg cccggtgatc
     6301 tttttacgca gaagcgtttt ttccgcctgc atcaacgcgt acggatgtga tgcggcggtc
     6361 ctgatctccg cgagagaaac accgggtttt gccgcgaggc aaagcgagac cggccattcc
     6421 acttcatcga ggatatgcag cttgctttcg taaataagac ggtaggtctc gtaaaaatgt
     6481 ccggcgacgg tgttcgagca ggcgagcaaa gtgtagtcgg agagattttc ttccgcccgc
     6541 gcgatcgcgc tgcgcatgtc gcctgtgtga agcatcaccg cgtcttcgcc gaaataccgg
     6601 cgcgcagcgt gatgatgaaa tgaattttcc cttgcgggaa tagaaatacg aagtggtctc
     6661 atgttttctg aaataaaaac gtcccggcaa ataccgggac gtctggttgg acaaaagcaa
     6721 tgctcacccg gcaggttgtt tcagcctgca gaaaaaataa tagccgcgat gagaatgcct
     6781 tgatttcatt tttcgttcag aggagcggta tgtttttcgc tttgcattgt tcgatcacgg
     6841 tgtcgggcca gagcccgggc tgcacttcac cgatgtgttt tttacgcagc agcagcatac
     6901 acaggcgcga ctggccgatc ccgccgccga tcgtcagcgg cagttttcct tctaccagcg
     6961 aacggtgcca cggaagttcc agccgccttt ccaggccgag cagcctgagc tggtgcagca
     7021 gcgcctcgcg gtcaacacgt atgcccatgg acgagatctc gaacgcgcgc cccagctccg
     7081 ggttccacac aaggatgtcg ccgttgagtc cgcaacggtc ttcactggtg gccgtgctcc
     7141 agtcgtcgta atcgggcgca cggagatcgt gctccttccc gtctttcagc tttccgccta
     7201 tgccgatgat gaacacggcg cctttttcgc ggcagacttt atcttcccgc tctttcggtg
     7261 acagctccgg gtgcagctca cgcaggcgtt ccgcatgcac aacgtgaatg tgcggcggaa
     7321 ggaacgccgg aagcgcagag tatttttccg aaagaagctc ctgcgtttca cgtatacagc
     7381 tgtagatgtc ctgcacggtt tcgtagagaa acaccaggct gcggtctttc ctgcatatca
     7441 ctttttccca gtcccactgg tcgacatacg cgctgtgaat gttgctcagc tcttcatccg
     7501 ggcgcagcgc atgcatgtcg gtgatgattc cctccccggg cggacagccg agtgaggcga
     7561 gctttcgtcg cttccatttc gcaagcgaat gcaccacctc cgcaacaccg tcacgttggg
     7621 ccgtgagcgc gaaagcaacg gggcgttcga cgccgttgag gtcgtcgttc tctcccgtac
     7681 cgcggaatac ggcaatgggc g
//
LOCUS       DASTSF010000533         4258 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1651535,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000533 DASTSF010000000
VERSION     DASTSF010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4258
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1651535"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1345
                     /locus_tag="VFU15_15200"
     CDS             <1..1345
                     /locus_tag="VFU15_15200"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015809133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEU4719189.1"
                     /translation="VAVKGTQVDGHEFIGKAISLGCAAVVCEKFPEELNEKITYVKVN
                     DCAEALGTIASNFYDNPSSSLKLVGVTGTNGKTTTVTLLFNLVRAMGIKAGMLTTVRN
                     QVNSEEISATHTTPDAVELNRLLRKMVDAGCRYAFIEVSSHSVVQHRISGLEFAGGVF
                     TNITHDHLDYHKTFGEYIKAKQGFFSQLPSSAFALVNKDDANASVMVQNSRAVKKTFS
                     LHTMADFKCRILENQFSGLHLNIDGTDIWTKLIGSFNASNILAVYATAVLLGFEKMNV
                     LTTLSTLGAVEGRFQYIRNDNGVTGIVDYAHTPDALQNVLKTIQDIRNGNEKVITIVG
                     CGGDRDAAKRPVMARIAAELSDCVILTSDNPRSENPETIIGQMKKGVEGPLAKKTLAI
                     TDRREAIRTAVAFAAPGDIILVAGKGHEKYQEISGVKHPFDDMEILKENLNKANA"
     gene            1347..2627
                     /gene="mraY"
                     /locus_tag="VFU15_15205"
     CDS             1347..2627
                     /gene="mraY"
                     /locus_tag="VFU15_15205"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010249105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEU4719190.1"
                     /translation="MLYYLFTWLDKMYDVPGAGVFRYISFRAAGAVFISLLISLVFGK
                     RIINILRRKQVGETVRDLGLHGQKEKEGTPTMGGLIILSAIFIPTLLFAKLQNVYIIL
                     MLISTVWLGVIGFLDDYIKVFKKDKQGLHGKFKVMGQIGIGLIVGCTLYFSHSVVIRE
                     KLSTDKKELLSLSDDPGKLNNACGDVGFPEYSKHDTCSLKTTIPFLKNNEFDYASITG
                     VFGEGGRKWAWIVFIPIVIFIVTAVSNGANVTDGIDGLAAGTSAIIGVVLGILAYVSG
                     NTIFSDYLNIMYIPDAGELVVFIAAFVGACVGFLWYNSFPAQVFMGDTGSLALGGIIA
                     VFAIAIRKELLIPIICGIFLVENISVILQVAVFKYRKKKHGIEYARAHRLFLMSPLHH
                     HYQKKGLHESKIVFRFLIVAIMLGVFAIVTLKLR"
     gene            2634..3968
                     /gene="murD"
                     /locus_tag="VFU15_15210"
     CDS             2634..3968
                     /gene="murD"
                     /locus_tag="VFU15_15210"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017843240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEU4719191.1"
                     /translation="MKRIVILGAGESGTGAAVLARQKGFDVFVSDKGAISEKHKNVLS
                     LHEIRWEEKKHSEELILDADEVIKSPGIPDKVELVKKIHGLGIPVISEIEFAGRYTDA
                     KMICITGSNGKTTTATITHHILQKAGLNVALGGNVGTSFAKLVAEGNHDYYVLELSSF
                     QLDGMFDFRADIAVLTNITPDHLDRYDYDLGKYTDSKFRITQNQRREDAFIWCLDDEI
                     TRTEIVKRKINARSYPFSIKEKVEEGAWLNEHELIININNTQLSMTIEELALQGKHNV
                     YNSMAAGIASRLLEVRKEAIRESLSDVQNVEHRMEFVARINNIDFVNDSKATNVNSTW
                     YALESINTPVVWIVGGVDKGNDYSALTELVKAKVKAIVCLGKDNAKIRKAFGNMVEVF
                     TEAATAQEAVSQSYRIAKKGDTVILSPACASFDLFENYEDRGRQFKAAVRAL"
     gene            3983..>4258
                     /locus_tag="VFU15_15215"
     CDS             3983..>4258
                     /locus_tag="VFU15_15215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719192.1"
                     /translation="MNGSVQIQEQRYAELLSARLKVQGDDAHHFETVGVYNNVAWINH
                     SIATTVELTWLALRDVPGPLALIIGGIDRGHDHEKLKMFVKQKVPVVI"
BASE COUNT         1095 a         1070 c         1197 g          896 t
ORIGIN      
        1 cgtcgcggtg aaaggaacgc aggtcgatgg tcatgaattc atcgggaaag cgatctcgct
       61 cggttgtgcg gctgtggttt gtgagaaatt cccggaagag ctgaacgaaa agatcacgta
      121 tgtcaaagtg aacgattgcg cggaagcact cggcaccatc gcttcgaatt tttacgacaa
      181 cccttcttct tcgctgaagc tcgtcggtgt aaccggaaca aacgggaaaa ccacgacggt
      241 cacgctgctg ttcaacctcg tgcgggcgat gggaataaaa gcaggaatgc ttacgactgt
      301 ccgcaaccag gtgaacagcg aagaaatttc cgcgacgcac acaacgccgg atgcagtgga
      361 gctgaacagg ctgctgcgga aaatggtgga cgcgggatgc cggtatgcat ttatagaagt
      421 gagctcgcac agtgtcgtgc agcacaggat cagcgggctc gaattcgcgg gcggcgtgtt
      481 cacgaacatc acgcatgatc atctcgatta ccacaagaca ttcggcgaat acataaaagc
      541 gaagcagggt tttttcagcc agcttccttc ttccgcgttt gcgctggtca ataaagacga
      601 tgcgaacgct tccgtcatgg tgcagaacag cagggccgtg aaaaaaacat tttcgctgca
      661 tacgatggcg gatttcaaat gccgcattct cgaaaaccag ttttccggtc ttcacctgaa
      721 catcgacgga acggatatct ggacgaagct catcggcagc ttcaatgcca gcaacattct
      781 cgccgtgtac gccaccgcgg tgctgctcgg ctttgaaaag atgaatgtgc tgacgacgct
      841 gagcacgctc ggtgcggtgg aaggaaggtt ccagtatatc cgtaacgaca acggcgtaac
      901 cggcatcgtt gattacgcac atacgccgga tgcactgcag aatgtgctga agaccatcca
      961 ggatatccgc aacggaaatg aaaaagtgat cacgatcgtc ggatgcggcg gcgaccgcga
     1021 tgcggcgaag cgcccggtga tggcccggat cgctgcggag ctgagcgatt gtgtgatcct
     1081 tacgtcggac aacccgcgtt ccgaaaaccc ggaaaccatt atcgggcaga tgaaaaaagg
     1141 agtggaaggt ccgctcgcaa aaaaaacgct cgcgatcacc gaccggcgcg aagcgatacg
     1201 tacggcggtt gcattcgccg cgcccggcga catcattctc gttgcgggaa aaggtcacga
     1261 aaaataccag gagatcagcg gggtgaagca cccgttcgac gatatggaaa tattaaaaga
     1321 aaacctgaac aaagccaacg cctgacatgc tgtactatct cttcacatgg ctcgataaaa
     1381 tgtatgacgt ccccggcgca ggtgtattca ggtacatctc gttccgcgcg gcgggtgctg
     1441 ttttcatttc gctgctgatc tcgctcgtgt tcgggaaacg catcatcaat atcctgcgga
     1501 gaaagcaggt gggagaaacc gtgcgcgatc tcggtctgca cgggcagaaa gaaaaggaag
     1561 ggacgccgac gatgggcgga ctgatcattc tctcggcgat tttcattccc acgctgctgt
     1621 tcgcgaaact gcagaacgta tacatcattc tcatgctcat ctccaccgtg tggctgggcg
     1681 tgatcggttt tctcgatgat tacattaaag ttttcaagaa ggacaagcag ggactgcacg
     1741 gaaaattcaa ggtgatgggg cagatcggca tagggctgat cgtcggctgc acgctttatt
     1801 tcagccattc tgttgtcatc cgcgaaaaac tttcgacgga taaaaaagaa ttgctttccc
     1861 tttccgatga tcccggcaag ctgaacaacg cgtgcggtga tgtcggtttc ccggaatatt
     1921 cgaagcacga tacctgctcg ctgaaaacca cgatcccgtt cctgaagaac aatgaattcg
     1981 attacgcaag catcacgggt gtgttcggcg aaggcgggcg caaatgggcc tggatcgttt
     2041 tcattcccat cgtgatcttc atcgtcaccg ctgtttcgaa cggcgcgaac gtgacggatg
     2101 gcatcgacgg gctcgcagca ggaacatcgg cgatcatcgg cgtcgtgctg ggcatcctcg
     2161 cttatgtatc cggtaatacc attttctccg attacctcaa catcatgtat atccccgacg
     2221 cgggcgagct cgtggtgttc atcgcggcat tcgtgggcgc gtgtgtcggg ttcctctggt
     2281 ataattcttt tcccgcgcag gtgttcatgg gcgataccgg aagcctcgcg ctcggcggca
     2341 tcatcgcggt gttcgcgatc gcgatacgca aggagcttct cattcccatc atctgcggga
     2401 tttttctcgt ggaaaatatt tccgtcatcc tccaggtggc ggttttcaaa taccgcaaga
     2461 aaaaacacgg catcgaatac gcacgtgcgc acaggttgtt cctgatgtcg ccgctgcacc
     2521 atcattacca gaagaaaggg ttgcacgaat cgaagatcgt tttccggttc ctgatcgtgg
     2581 cgatcatgct gggtgtgttt gcgattgtca cgctaaagct gcgctgagga aggatgaaaa
     2641 ggatcgtcat tctcggtgcg ggtgaaagcg ggacaggagc agctgtgctg gcccggcaga
     2701 aaggattcga cgtttttgtc tccgacaaag gcgcgatatc cgaaaagcat aagaacgttc
     2761 tttctcttca tgagatcagg tgggaggaaa aaaagcattc ggaagaactg atcctggatg
     2821 cggatgaagt gatcaagagt cccggcatcc cggataaagt cgagctggtg aagaaaattc
     2881 acggcctcgg catcccggtg atctccgaga tcgaattcgc gggaagatat acggacgcga
     2941 agatgatctg catcacggga agcaacggga agacgaccac ggcgaccatc acgcatcaca
     3001 tcctgcagaa agcaggactg aacgttgcgc tcggcggaaa tgtgggaacg agcttcgcga
     3061 agctggtggc ggaaggcaat cacgattact acgtgctcga gctgagcagc ttccagctcg
     3121 acggcatgtt cgatttccgc gccgacatcg ccgtgctgac caacatcacc cccgaccacc
     3181 tcgaccgcta cgattacgac ctcggaaaat acacggattc gaaattccgc atcacgcaga
     3241 accagcgcag ggaagacgca ttcatctggt gcctcgatga tgagattacg agaacggaaa
     3301 tcgtgaaaag aaaaataaat gcgcgcagct atcctttttc aattaaggaa aaagtggagg
     3361 aaggcgcgtg gctgaatgaa catgaattaa taataaacat caataataca caactgtcta
     3421 tgaccatcga agaacttgca ctccagggaa aacacaatgt ctacaactca atggcggcag
     3481 gcattgcatc gcgcctgctc gaagtaagaa aagaagcgat aagagaatcg ctttcggatg
     3541 tacagaatgt cgagcaccgc atggaattcg tcgcgcggat caacaatatc gattttgtca
     3601 atgattcgaa agcgaccaac gtcaattcca cgtggtatgc gctcgaaagt atcaacactc
     3661 ccgtggtgtg gatcgtcggc ggagtggata agggcaacga ttattctgcg ctcaccgagc
     3721 ttgtgaaagc gaaagtgaag gcgatcgttt gtctcgggaa agacaacgcc aagatccgca
     3781 aggcgttcgg caatatggtg gaagtgttca ccgaagcggc gacggcgcag gaagccgtca
     3841 gccagtcgta ccgcattgcg aaaaaaggcg acacggttat tctttcgccc gcatgcgcga
     3901 gcttcgatct ttttgaaaat tatgaagacc gcggccgcca gttcaaagcg gcggtacggg
     3961 cgctctgaaa aacggaacgg gcatgaacgg cagcgtgcag atacaggaac agcggtacgc
     4021 agagcttctc agcgcaaggc tgaaagttca gggtgatgac gcgcatcact tcgagaccgt
     4081 cggcgtatac aacaacgtgg catggatcaa tcattccatc gccacgacgg tagagctgac
     4141 gtggctggcg ctgcgcgatg ttcccggtcc gctcgcgctg atcatcggcg gcatcgatcg
     4201 cggtcacgat catgaaaagc tgaaaatgtt cgttaagcag aaagtgcctg tggtcatc
//
LOCUS       DASTSF010000534         7408 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1226483,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000534 DASTSF010000000
VERSION     DASTSF010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 7408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7408
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1226483"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..849
                     /locus_tag="VFU15_15220"
     CDS             <1..849
                     /locus_tag="VFU15_15220"
                     /inference="COORDINATES: protein motif:HMM:NF023327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3419 family protein"
                     /protein_id="HEU4719193.1"
                     /translation="ADHDSFLSFLGFSESNERKVQYDSLRALLSPQAAAWWDAHFPLI
                     ARGVITQGKFENYFRYFRTRMLPLIHSKKDVAGLFREKEKGGQEIFYRTTWNTWRWRM
                     FFRIFFSRYVMGKYGRDPEFMREVNVSVSDYIFSKAENELRSDAAQENLFLKNIMTGN
                     FGARLPFYARKENFENIRNAMDRFHVFRGYAEDACRTFGKFSAFNLSNIFEYLSPQVT
                     REVAEGLVRNTVPGARFAYWNLMVPRKLSQACPGTLEYREEISDRLTERDMGFFYLHF
                     NLDVKK"
     gene            846..1556
                     /locus_tag="VFU15_15225"
     CDS             846..1556
                     /locus_tag="VFU15_15225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEU4719194.1"
                     /translation="MNRDILHLLLIGGIFFALFLAGEFLFHIVKVKGEYTRKFIHIST
                     GFITLLFPFFFTSHWYVLILCAGFAVILKLSLKLNMLKSINAIDRDSHGSISYPASVY
                     GTFLFAQYAYSQGFEPALYFVPILTLALCDPAAAITGKNLPWKPFKVGEGKKTVSGTL
                     AFFMSALALNFILVRVLTISHPGPCILMAATIAVFTTIAEAVSRKGLDNIFVPATAIL
                     LLWFFLRYTHLFISTSVA"
     gene            1553..2572
                     /locus_tag="VFU15_15230"
     CDS             1553..2572
                     /locus_tag="VFU15_15230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719195.1"
                     /translation="MKNKLALQTFFPSEDHPLFSSLPGKIYPASLFALQKAGDTINPE
                     FAAAGIAVTNENNETLGRLVLYANTGIRYNGKKTAIAGNYECVDDAGASLLLLESAAA
                     FAKKEGFHILLGPANGTTWDTYRFCTGDLQELFFSEMVNLPYYPAQWKNAGFGVAAKY
                     VSTIDHSLKCDGEEVLAIEKKFLGSGFRIRNIDIAQYENELELLFPFLERSFAGNFLY
                     TPVSREIFLKKYTAVKPYLDPRSVFIAENPVGNPVAVAFCFPDHLNKKEKRLIVKTLA
                     RDPDPRYKGLGEVLGNLSQRFAKQNGYEAVIHALMYTENYSLKLSGNYAGKIFREYEL
                     YSKEL"
     gene            2569..3165
                     /locus_tag="VFU15_15235"
     CDS             2569..3165
                     /locus_tag="VFU15_15235"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEU4719196.1"
                     /translation="MKKAARLFACFLLFLLFTSALTVVFSPYGKQENFPYRSVVNSVE
                     INAPPDTVFHYLGNSAHAHNWSVFVDHITTLNADSFPDGTPGSRRRCFRQQNENGIRW
                     DEEVTQVEPGKKRQLTIYNMKEFPLMADGLATEQIYEPLKNGNACRLTFTVFYLHHSP
                     TFFETFKTYIAAYRINDIFKANLGNIKRIVEEENGYKG"
     gene            3149..4690
                     /locus_tag="VFU15_15240"
     CDS             3149..4690
                     /locus_tag="VFU15_15240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013453768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEU4719197.1"
                     /translation="MGTRDNVCDLFFSCAERSPGKAAIIDRDGSAETFGKLAEDVKAT
                     AAYFHSKGIRKGDRVLVFVPIGIPLYRTVLALFHMGAVAVFLDEWVSRERLEVCCRIA
                     KCKAFIAPFRLRALAFFIGELRKIPVWLGTRYTPATSADPQALTHSSDTALITFTTGS
                     TGIPKAADRTHGFLAAQFGALVDKIRPETTDVDMPVLPIVLLLNLGTGVTSVIADFNS
                     RKPETIDTEKIWMQIISSNVNRFVASPFVVEQMAASGFNRKTRVRFFTGGAPVFPFQA
                     ERMKKGLPGSSVEIVYGSTEAEPISTISAEELISEKNKSEKGLCVGKPYHGTTVRILP
                     VTEKIRSFRTEEELDSASLPTGKPGEIVVSGKHVLRHYIDNPEAEKQNKIFIGENVWH
                     RTGDSGFLDENGNLFLCGRCSSLITDEHGETLAPFLWEGWFLTLPGVSMGTIAQHDGK
                     IIAVIECAPEKENEVEKLILGKYPFSSVVFLPRLPRDPRHFSKIDYGKLKEILPKKQA
                     GRQNP"
     gene            4750..5991
                     /locus_tag="VFU15_15245"
     CDS             4750..5991
                     /locus_tag="VFU15_15245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016195468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU4719198.1"
                     /translation="MNFSFTENIRIALSAIRAQMLRAFLTILIIALGIGALVGILTAI
                     DVMKGSLNSNFASMGANTFTIRNREFRIRVGRRGHKPKRFRDITYHEAMDFKDRFDFP
                     STVSVSTMASMTATLKYKSNKSNPNVQVFGTDENYLATSGYELAEGRNFSREECLGGA
                     HVAIIGHDMVTTLFPDNEDPVGKEINVGAGTYIVIGTLKTKGTAMGFGGDKVAFLPLE
                     NVRQYFSRPDMTFSISVLARNSTMLDPTVSEATGLFRIIRKVRPGEDENFEITKSDSV
                     AATLIDNLQYVTLGATIIGVITLLGAAIGLMNIMLVSVTERTREIGIRKAIGATSSTI
                     RYQFLTEAIVICQLGGLFGIVLALIIGIGISFIMSASFVVPWLWIFLGLVICFLVGLI
                     SGFYPAAKASRLDPIDALRYE"
     gene            complement(6061..6510)
                     /gene="rplI"
                     /locus_tag="VFU15_15250"
     CDS             complement(6061..6510)
                     /gene="rplI"
                     /locus_tag="VFU15_15250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008152407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEU4719199.1"
                     /translation="MEVILKQDVRNLGYKNDIVKVKDGYGRNFLIPRGLAIVADLTAK
                     KVHSENMRQSAHKEAKIKAEAEKSAAKIQEMTVKVGAKAGENGKIFGSVTGVQLADAL
                     RKLGFEVERRNISIENEANIKTLGTYNAKVRLHKEVVAPFRFEVVSE"
     gene            complement(6529..6798)
                     /gene="rpsR"
                     /locus_tag="VFU15_15255"
     CDS             complement(6529..6798)
                     /gene="rpsR"
                     /locus_tag="VFU15_15255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005637107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEU4719200.1"
                     /translation="MAENSSEIRYLNPPSVEVKKKKYCRFKKAGIKYIDYKDADFLLK
                     FINEQGKILPRRLTGTSVKYQRKMSSAIKRARHIAILPYVADQLK"
     gene            complement(6824..7201)
                     /gene="rpsF"
                     /locus_tag="VFU15_15260"
     CDS             complement(6824..7201)
                     /gene="rpsF"
                     /locus_tag="VFU15_15260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008244394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEU4719201.1"
                     /translation="MYHYETVFILTPVLSEDQAKEAVGKFRKIIKDLGANIIHEESWG
                     LRKLAYQIQKKSTGHYHLFEYTATGGSAVAELELAFKRDERVLRFMTVGLDKHGVAYA
                     EKRRNKAKAAKKEAAAKSAEKTA"
BASE COUNT         1704 a         2006 c         1882 g         1816 t
ORIGIN      
        1 gcggatcatg attcgttcct ttcttttctc gggttttcag aaagcaatga acgtaaagtg
       61 caatacgatt ccctgcgcgc gctcctttct ccgcaggctg cggcgtggtg ggatgcgcat
      121 ttccctctca tcgcgcgcgg cgtgatcacg cagggaaaat tcgagaacta tttccgctac
      181 ttccgcacgc gcatgcttcc gctcatccat tcaaaaaaag atgtggccgg gcttttcagg
      241 gaaaaagaaa aaggcgggca ggaaattttc taccgcacaa catggaacac gtggagatgg
      301 cgcatgttct tccggatctt cttcagccgt tacgtgatgg ggaaatacgg gcgcgacccc
      361 gagttcatgc gcgaggtgaa cgtttccgtt tccgattata ttttttccaa agcggaaaat
      421 gagctgcgct cggatgcggc gcaggaaaat cttttcctga aaaatatcat gacgggaaat
      481 ttcggagcgc gtttgccttt ctatgcacgg aaagaaaatt tcgaaaatat ccggaatgcg
      541 atggatcgct tccacgtttt ccgcggatat gcagaagatg catgccgcac gttcggaaaa
      601 ttttccgcat tcaacctctc gaacattttc gaatatcttt ctccgcaggt gaccagggag
      661 gttgccgaag gccttgtccg caatacggtg ccgggcgcga gattcgccta ctggaacctg
      721 atggtgccgc ggaaactttc ccaggcatgt cccgggacgc tggaataccg ggaagaaatt
      781 tcagaccgtc tcacggaacg cgacatggga tttttctacc tccattttaa cctcgatgta
      841 aaaaaatgaa ccgggatatc cttcatcttc tcctcatcgg cggaattttc ttcgcgctgt
      901 tcctcgccgg ggaatttctc tttcacatcg ttaaagtgaa aggagaatac acgcggaaat
      961 tcatacacat ttccaccgga ttcatcacgc tgctttttcc tttcttcttc acaagccact
     1021 ggtacgtgct cattctctgc gccggcttcg ccgtcattct caaactgagc ctgaagctga
     1081 acatgctgaa atccatcaac gccatcgacc gcgattcaca cggcagcatt tcctacccgg
     1141 cttctgttta cggcaccttc cttttcgcgc aatatgcata ttcgcagggg ttcgaacctg
     1201 cgctctactt tgtcccgatc ctcacgcttg cgctttgtga tcctgccgct gcgatcaccg
     1261 ggaaaaattt accatggaaa ccgtttaagg tcggtgaagg aaagaagacc gtttcgggta
     1321 cgcttgcatt tttcatgtcc gcgctggcgc tgaacttcat cctggtccgc gtactgacga
     1381 tctcgcaccc cggtccctgc atcctcatgg ccgccaccat tgctgttttc acaacgatcg
     1441 cggaggcggt aagccggaaa gggctggaca atatcttcgt tcctgcgacg gctattctcc
     1501 tgctttggtt tttcctgcgc tatactcacc tgttcatttc aaccagcgtc gcatgaaaaa
     1561 caaactggcc ctgcagactt tttttccttc ggaagatcac ccgctgttca gcagcctgcc
     1621 ggggaagatc tatcccgctt cgctctttgc actgcaaaaa gcgggtgaca ccatcaaccc
     1681 ggaattcgcg gccgccggaa ttgcagtgac gaatgaaaac aatgaaacgc tcggccgcct
     1741 tgtgctttat gcgaacacag gaatccggta caacggaaag aaaacggcaa ttgcgggaaa
     1801 ttacgaatgc gtggatgatg ccggagcatc tttgctgctg ctggaatccg cagcggcttt
     1861 tgcaaaaaaa gaagggtttc atatcctgct cggaccggcg aacggaacga catgggacac
     1921 gtaccgtttc tgcacgggag atctgcagga attgtttttt tcggaaatgg tgaaccttcc
     1981 gtactatccc gcacaatgga aaaatgccgg gttcggcgtt gccgcaaagt atgtttccac
     2041 gatcgatcat tcgctcaaat gcgacgggga agaagtgctt gcgattgaaa agaaattcct
     2101 cggcagcggc ttccgcattc gcaacatcga catcgcacag tatgagaatg agctggagct
     2161 gctcttccct ttcctcgaac ggtcgttcgc cggcaatttc ctttacaccc ccgtgagccg
     2221 tgaaatattc ctgaaaaaat acacggcggt aaaaccttat ctcgatcccc gttctgtttt
     2281 cattgcagaa aaccccgtag gaaacccggt agctgttgca ttctgttttc ccgatcacct
     2341 gaacaaaaaa gaaaaacggc ttatcgtcaa aactcttgca cgtgatcccg atccgcgcta
     2401 caaaggactc ggggaagtgc tgggaaacct gtcgcagcgt tttgcgaagc agaacgggta
     2461 cgaagcagtg atccatgcgc tcatgtatac ggaaaactat tcgctgaagc tgagcggaaa
     2521 ctacgccggg aaaattttcc gcgagtacga actttattcg aaagagctat gaaaaaagct
     2581 gcgcggcttt ttgcctgttt tctattgttc ctgcttttta cttccgccct aacggtggtt
     2641 ttcagtccgt acggaaaaca ggaaaatttc ccctatcgtt ctgtcgtaaa cagcgtcgag
     2701 atcaatgcgc cgccggacac ggttttccat tatctcggca attcggcaca cgcgcacaac
     2761 tggtctgtgt tcgtggatca catcaccacg ctgaatgcgg acagttttcc cgacggcaca
     2821 cccggttcgc gccggcgctg cttccggcag caaaatgaaa acggcattcg ctgggacgaa
     2881 gaggtgacgc aggtggaacc cgggaaaaaa cgacagctga cgatctacaa catgaaagaa
     2941 tttccgctca tggccgacgg tctcgccacc gaacagatct acgagccgct gaagaacggg
     3001 aatgcgtgcc gcctcacctt caccgttttt tacctgcatc attcgccgac atttttcgaa
     3061 acgttcaaga cctacatcgc ggcatacagg atcaacgaca ttttcaaagc caacctcggc
     3121 aacatcaaac gcatcgtgga agaagaaaat gggtacaagg gataatgtct gcgatctttt
     3181 cttttcctgc gcggaaagat ctcccgggaa agccgccatc atcgatcgcg acggaagcgc
     3241 cgagactttc ggcaaactcg ctgaggacgt aaaagctact gcggcgtatt ttcattcgaa
     3301 gggaatacga aaaggcgacc gcgtgctggt tttcgttccc ataggcattc cgctgtacag
     3361 gactgtgctc gcgctttttc acatgggcgc tgttgctgtt tttctcgatg aatgggtgag
     3421 cagggaacgg ctggaggtgt gctgccgcat cgcaaaatgc aaagcgttca tcgccccgtt
     3481 ccggttacgc gccctcgcct tcttcatcgg ggagctgcga aaaattcccg tctggctcgg
     3541 aacacgttac acaccggcga cttctgcgga tccacaggca ctgacgcatt cctccgatac
     3601 cgcgctcatc acgttcacca ccggctcaac gggaattccc aaagccgccg accgcacgca
     3661 tggattcctg gctgcacaat tcggggcgct ggtcgacaag ataaggcccg aaacgaccga
     3721 cgtggatatg ccggtcctcc cgatcgtgct gctgctgaat ctcggcacgg gcgtcacgtc
     3781 cgtcatcgcg gatttcaatt cgcgcaaacc cgaaacgatc gacacggaaa agatctggat
     3841 gcagatcatt tcttcgaatg tcaaccgctt tgtcgcctcg ccttttgtcg tggagcagat
     3901 ggccgcatcc ggcttcaacc ggaaaacacg cgtgcgtttt ttcaccggcg gcgcaccggt
     3961 ttttcctttc caggcggagc gcatgaaaaa aggattgccc ggttcatccg tggaaattgt
     4021 ttacggttca acggaagccg agccgatcag cacgatctcc gcggaagagc tgatcagcga
     4081 aaaaaacaag tcggaaaaag gattgtgcgt gggaaaaccg taccacggca caaccgtgcg
     4141 cattctgccg gtgacagaaa aaatacgctc tttcagaaca gaggaagaac tggacagcgc
     4201 gtcacttccc accgggaagc cgggcgagat cgtcgtttcc gggaagcacg tgctgcgtca
     4261 ttacatcgat aatcccgaag cggaaaaaca gaacaagatt ttcatcggtg aaaatgtatg
     4321 gcaccgcacc ggtgattccg ggtttctcga tgagaacggg aacctgttcc tctgcggacg
     4381 atgcagctcg ctcattacgg atgaacacgg tgaaacgctc gcccctttcc tctgggaagg
     4441 ctggttcctt acgctgcccg gcgtttcgat gggaaccatt gcacagcacg acggaaagat
     4501 catcgccgtc atcgaatgtg cgcctgaaaa agagaacgag gttgagaagc tgatccttgg
     4561 gaaatacccg ttctcttctg tcgtgttcct tccgcgtctt ccccgcgacc cgcgccattt
     4621 ttcgaagatc gattacggga agctgaaaga aatcttaccg aaaaagcagg caggcaggca
     4681 aaacccgtaa cggaaaacgg gaaacacgaa aacgggagca tcgcttttcc cctacctttg
     4741 cagggttcaa tgaacttttc cttcaccgaa aatatccgca tagcgctttc cgcgatccgc
     4801 gcgcagatgc tgcgtgcgtt cctcacgatc ctcatcatcg cgctgggcat cggtgcactg
     4861 gttggaattc tcaccgccat cgacgtgatg aaaggatcgc tgaacagcaa tttcgccagc
     4921 atgggcgcga acacgttcac gatccgcaac cgcgagttcc gcatacgcgt gggccgacgc
     4981 gggcacaaac ccaaacggtt ccgcgacatc acctaccatg aagcgatgga tttcaaggac
     5041 cgtttcgatt ttccttccac ggtttccgtt tccacgatgg cgtcgatgac cgcgacgctg
     5101 aaatataaat cgaacaagag caacccgaac gtgcaggtgt tcggaacgga cgagaattac
     5161 ctcgccacat ccggctacga gctggcggaa ggccggaatt tttcgcgtga agaatgcctg
     5221 ggcggggcgc acgtggccat catcgggcac gacatggtga cgacgctttt tccggacaac
     5281 gaagacccgg tgggaaaaga gatcaacgtg ggcgccggaa cttacattgt catcggtaca
     5341 ctgaaaacga aagggaccgc gatgggcttc ggcggcgaca aagtggcttt ccttcccctg
     5401 gaaaatgtac ggcagtattt ttcccgtccc gacatgacgt tttccatcag cgttcttgcg
     5461 cgcaattcca cgatgctcga tccgaccgtc agcgaagcga caggactgtt ccgcatcatc
     5521 cgcaaagtgc ggccgggcga agacgaaaat ttcgagatca cgaaaagcga cagcgtcgcg
     5581 gcaacgctca tcgacaacct gcagtacgtc acgctcggtg caacgatcat cggcgtgatc
     5641 acgctgctcg gtgccgcgat cggtttgatg aacatcatgc tcgtttccgt aacggaacgt
     5701 acgcgcgaga tcggcatacg caaggccatc ggcgcaacga gctccaccat ccgttaccag
     5761 ttcctcaccg aagcgatcgt gatctgccag ctcggcgggt tgttcgggat cgttctcgcg
     5821 ctgatcatcg gcatcggtat ttccttcatc atgagcgcgt cattcgtcgt gccctggctg
     5881 tggatctttc tcggcctggt gatctgcttc ctcgtcggac ttatttccgg tttttatccc
     5941 gctgcaaaag cttcccgcct cgacccgatc gatgcgctgc gttacgaatg atctgcgtac
     6001 cggcgaacac aaaaaaaggc tgtccattcc tgaacagcct ttcgtttttt gcgtggagat
     6061 ttactcgctg accacttcga acctgaacgg ggcgacaact tctttgtgca ggcgtacttt
     6121 tgcgttgtac gttcccagcg ttttgatgtt ggcttcgttt tcaattgaaa tgttgcggcg
     6181 ttccacttcg aagccgagct tgcgaagcgc atctgccagc tgaacaccgg taacggatcc
     6241 gaagatcttc ccgttctcgc cggctttcgc accgaccttc acggtcattt cctggatctt
     6301 tgcagcgctc ttttcagctt ccgctttgat cttcgcttcc ttgtgcgcac tctggcgcat
     6361 gttttcggag tgaactttct ttgccgttag atcggcaacg atggcaagtc cgcggggaat
     6421 aagaaaattc cttccgtaac catcttttac cttgacgatg tcgttcttgt aaccaaggtt
     6481 cctgacatcc tgcttgagaa ttacttccat gttaatgtcc tccttgtttt acttgagttg
     6541 atcggctacg tacggcagga tcgcaatgtg acgcgcgcgt ttgatggcgg acgacatttt
     6601 gcgctggtat ttcacggatg taccggtgag acggcgggga agaatttttc cctgctcgtt
     6661 gatgaacttc agaaggaagt cagcgtcttt gtaatcgatg tatttaatac cggctttctt
     6721 gaagcggcag tattttttct tttttacctc aaccgacggc gggttcaggt agcggatttc
     6781 tgatgaattc tcagccatga ctgattttgc gttgttgagt taattaagcg gttttttctg
     6841 ctgattttgc agcagcttct tttttagcgg ctttcgcttt gttgcggcgt ttctccgcgt
     6901 aagctacacc gtgcttgtcg agtccgacag tcatgaaacg cagcacgcgc tcatcgcgct
     6961 tgaatgcgag ctcgagttca gcaacagcgc ttccacccgt tgcggtgtac tcgaaaagat
     7021 gatagtgacc ggtggacttc ttctggatct ggtaagccag ctttctcagg ccccaggatt
     7081 cctcgtgaat gatgttcgcg ccgagatcct tgatgatctt gcggaacttg cccaccgcct
     7141 cctttgcctg gtcttcagac aaaacgggag tcaaaatgaa gacggtttcg taatggtaca
     7201 tttggtttta ttttggtttt accctccggg cacttcccgg aaagggaggg caaaggtaag
     7261 aaaacccagg gcttatttca aaaagaatac gattagtttg gactgaacct gctgaagctt
     7321 caatggacta ataaacagac tattacaggt tattttaccg tattttattt gaaataatgg
     7381 catttcaatg ccccggccag gcttttct
//
LOCUS       DASTSF010000535         5599 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1807725,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000535 DASTSF010000000
VERSION     DASTSF010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5599
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1807725"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..421
                     /locus_tag="VFU15_15265"
     CDS             <1..421
                     /locus_tag="VFU15_15265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719202.1"
                     /translation="LGGYTYMDPVSLRWNPAKDTLVNTSHENILKYRYRNIAKFDGEV
                     TYKKFSFGVSMRYNSFMENIDKIFEEATIALPGIKEYRDRFHRSDWVFDMRVAYQLSK
                     EAKVSFVVNNVFNYEYTQRPADVQPPVNFAVQVSIKF"
     gene            complement(548..1273)
                     /gene="truB"
                     /locus_tag="VFU15_15270"
     CDS             complement(548..1273)
                     /gene="truB"
                     /locus_tag="VFU15_15270"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007646708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEU4719203.1"
                     /translation="MTEKKPFDFVAGEVLLIDKLQGWTSFDAVNRIRSMIKRHLNLKV
                     KVGHAGTLDPLATGLLILCTGKFTKRIEEFQAQEKEYTGTFVVGATTPSFDLEHEVNE
                     HFPVAHITDEMIRAATKKFLGETEQVPPMFSAIKVEGKRAYELARKGEGSVIPPRKIT
                     ITGFEITGIERSEADDAPVIAVHFRVSCSKGTYIRSLARDFGRALDSGAYLSALRRTR
                     IGEFRIEDAMTIDQLGLKVMGRS"
     gene            complement(1273..2073)
                     /locus_tag="VFU15_15275"
     CDS             complement(1273..2073)
                     /locus_tag="VFU15_15275"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014085170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEU4719204.1"
                     /translation="MNLFHSILLGIIEGLTEFVPVSSTGHMVIVSSALGIANLEFTKT
                     FTVAIQLGAILSVVVLYWKKFINLKDIRFYVKLAIAVIPALVFGKLLADFIDEKLGNP
                     IFIAGSLLAGGIVLLFVDNWFKNPRITDEKEISYMKAFWIGCWQVLAIIFPGMSRSAS
                     SIIGGMQQKLTRGVAAEFSFFLAVPTMFAATGYKLFKHVKTQGMFTGEEIKLLAVGNI
                     VAFVVALFAIKVFIDLVKRYGFRMWGIYRIILGVAVLVINHFFPLTVV"
     gene            complement(2153..2452)
                     /locus_tag="VFU15_15280"
     CDS             complement(2153..2452)
                     /locus_tag="VFU15_15280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3098 domain-containing protein"
                     /protein_id="HEU4719205.1"
                     /translation="MAKKISVKKDGTTSPASRGKEAAPAAFVFGKDNYMIMLAGVAVL
                     VIGYLLMIGGGSDDPNKFNPEIFDARRVTIAPVTLLIGFLVVLYGIMRKPKQSAE"
     gene            complement(2586..3464)
                     /locus_tag="VFU15_15285"
     CDS             complement(2586..3464)
                     /locus_tag="VFU15_15285"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HEU4719206.1"
                     /translation="MSDTYSRRRIRSSSFTIVISISLVLIMLGLLSVLVLKAKEVSDY
                     VRENIKITVFLDQDAKDVDIIALQKSIDAEPWRKSTEFVSRENAAKKLQKDLGEDFIN
                     TLGYNPLQPSIEIFLKAGYANSDSISWIEQDLRDDARVKDVLYQKDMVSAVNERLGTI
                     SLVILVFSSALLIVAVGLIFNTIRLAIYSQRFLIRTMQLVGATQGFIRSPFVWRGIIN
                     GVIGAIIAILVTTGVLFSIDRHFRELNLIRFADVNTLAALCGLQIVFGIIITWISTSL
                     AVRRFLRQSVDELYSN"
     gene            3916..4956
                     /locus_tag="VFU15_15290"
     CDS             3916..4956
                     /locus_tag="VFU15_15290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012144082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HEU4719207.1"
                     /translation="MAQKKKSEAELTATEEAIVAKSKLPLVFISHDTKDAEIAEAFSK
                     LLSSVSAGVLKSFRSSDKKGTQGIEYGVEWFPEIMKKLDEASDIVCLLTHNSVDRPWI
                     LYEAGVAKGKLSTTVLGIAVGIPLNKANNGPFAQFQNCADDEDSLTKLVIQLVRRIPN
                     SEPDEEIIKEQVKTFRDKLSGMLKKQPKVEEKTKELMGDASIAKLFEEVKIMFQDLPS
                     RIDKRLDPEMIGMKRRRRFHPKMMDELFHISMKVGDPNISFLMMISLVKDEMPWLYEI
                     GIETYRSLKTAKTKSDKQKAISQYHQALELMGHPIFVEMYARSEESYMMMKEMRHLMH
                     RYFDQFFLDGKG"
     gene            5074..>5599
                     /locus_tag="VFU15_15295"
     CDS             5074..>5599
                     /locus_tag="VFU15_15295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008508400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEU4719208.1"
                     /translation="MSQDEFSVTIHMVSSLDGIIARKDNSISWFETSDKYDKGVDSED
                     PEEFLKTIDCYVMGSKTYELALELSKKYGWAYGDKPTTVLTNRKLQTNRHNIEFYSGD
                     LNKFVNERLKPKFKNVWVVGGSMLTKDLIRLKLADEIRVSILPIILGDGLLFFDHIGQ
                     EQALHLKDSKAYKNG"
BASE COUNT         1553 a         1316 c         1461 g         1269 t
ORIGIN      
        1 gctcggcggc tatacgtaca tggatcctgt ttcgctgcgc tggaatcctg cgaaagacac
       61 gctggtgaat acatcgcacg agaatattct caagtaccgt taccgcaaca ttgcgaagtt
      121 cgacggcgaa gtgacgtaca aaaagttctc cttcggcgtg agcatgcgtt acaacagctt
      181 catggagaac atcgacaaga tcttcgaaga agcgacgatc gcgctccccg gcatcaagga
      241 ataccgcgac cgtttccatc gcagcgactg ggtgttcgac atgcgcgtgg cgtaccagct
      301 ttcaaaagaa gcgaaggttt cattcgtcgt gaacaacgtc ttcaactacg aatacacgca
      361 gcgtcccgcc gatgtgcagc ctcccgtgaa ttttgcggtg caggtttcca ttaaatttta
      421 acggcggaaa gccacacgct cttttgtgct gaaagctgcg cacaattctt ttgaaccttc
      481 gtgaaacctt tacggacttt gtctttactg aacggccggg cccaatactg gcaacccgga
      541 tcttccatca gcttctgccc atcactttca gtcccagctg gtcgatcgtc atcgcatctt
      601 cgatgcggaa ttccccgatg cgcgtgcggc gcagtgcgga aagataagcg ccggaatcca
      661 gcgcacggcc gaaatcgcgt gcaagagacc ggatgtacgt tcctttcgag caggacacac
      721 ggaaatgaac agcgatcacg ggcgcatcat cggcttcgct gcgttcaatt cccgtgatct
      781 caaatcccgt gatcgtgatc ttccgcgggg gaatgacgga gccttcgcct tttctcgcga
      841 gctcgtacgc acgtttgcct tccactttta tcgcggagaa catgggcggc acctgctctg
      901 tttcgccgag aaatttcttg gttgcggcgc ggatcatttc gtccgtaatg tgcgcaaccg
      961 ggaaatgttc attcacctcg tgctcgagat cgaatgaagg cgtagtggcg ccgacaacaa
     1021 aagttcccgt gtattctttc tcctgcgcct ggaattcctc gatgcgcttc gtgaattttc
     1081 ccgtgcagag gatgagcagt cccgtcgcca gcggatcgag cgtaccggcg tgacccacct
     1141 tcactttcag gttgagatga cgtttgatca tgctgcggat gcggttcacc gcatcgaacg
     1201 acgtccagcc ctgcagcttg tcgatgagca gcacttcacc tgcaacaaaa tcaaaaggct
     1261 ttttttccgt catcacacaa ccgtgagcgg gaagaaatga ttgatgacca gcaccgccac
     1321 gccgagaatg atgcggtaga tgccccacat gcggaaaccg tagcgtttca cgagatcgat
     1381 gaacaccttt atcgcaaaaa gcgcgacgac aaatgcgacg atatttccga cggccagcag
     1441 tttgatctct tcgccggtga acatgccctg cgttttcacg tgcttgaaca gcttgtagcc
     1501 ggttgcagcg aacatcgtgg gaacggcgag gaagaaggaa aattcagcgg ccaccccgcg
     1561 tgtgagcttc tgctgcatac cgccgatgat ggacgaagcg ctgcggctca tgccgggaaa
     1621 aataatcgcg agcacctgcc agcagccgat ccagaacgct ttcatgtagc tgatctcttt
     1681 ctcgtccgta atccgcggat tcttaaacca gttgtcgacg aagagcagca cgatcccgcc
     1741 cgccagcagc gatcccgcaa tgaaaatggg attgccgagc ttttcatcga tgaagtctgc
     1801 gagcagcttt ccgaaaacaa gcgcaggaat aacggcgatc gccagcttga cgtaaaaacg
     1861 gatgtctttg aggttgatga attttttcca gtacagcacc actacggaaa ggatcgcgcc
     1921 gagctggatg gcaaccgtga atgttttcgt gaattccagg ttcgcgatgc cgagcgccga
     1981 agacacgatg accatgtgcc cggtggagga aacagggaca aactcggtaa gtccttcgat
     2041 gatgccgagg agaatggaat gaaaaaggtt cattgggaag atttacggtt tacgaggtgc
     2101 gatttacgat ttcaaaacca gtatccgtac gtcgtaattc gtccttcgta aattattctg
     2161 cagactgttt cggcttgcgc atgatgccgt acaggacgac aaggaacccg atgagcaacg
     2221 taacaggagc gatcgtaacc cgtcgcgcgt cgaagatctc gggattgaat ttgttcggat
     2281 catcggaacc gccgccgatc atgagaaggt aaccgatcac gagcacggcc acaccggcca
     2341 gcatgatcat gtaattatct tttccgaaaa cgaaagccgc aggagccgct tctttgccgc
     2401 gcgatgcggg agaagtcgtt ccgtcttttt tcaccgagat cttttttgcc atgactgcaa
     2461 ggttgtaaga atgcgtcctt tgcgggactg aacggcaaat atagcatgta atcgccaaat
     2521 ggacggaaat cgaaggcgcg gcaggggatt ttgggaaaat ttaggagggc cggcgccggg
     2581 ccgcgtcagt tgctgtacag ctcgtccacc gactggcgga ggaacctgcg cacggcaagc
     2641 gacgtgctga tccaggtgat gatgatgccg aaaacgatct gaagtccgca aagcgccgca
     2701 agcgtgttca cgtcggcgaa gcggatgagg ttgagctccc ggaaatgcct gtcgatgctg
     2761 aacagcacgc ccgtggtgac gaggatggcg atgatcgcgc cgatgacacc gttgatgatg
     2821 ccgcgccata cgaaaggcga acggatgaag ccctgcgtgg cgcccaccag ctgcatggtg
     2881 cggatgagga agcgctgcga gtaaatggcg aggcggatgg tgttgaagat aagtccgacg
     2941 gcgacgatga gcagcgccga actgaacacg aggatgacga ggctgatggt gccgagacgt
     3001 tcgttgacgg cgctcaccat gtctttctgg taaagcacat ccttcacgcg cgcgtcgtcg
     3061 cggaggtcct gctcgatcca gctgatgctg tcggaattgg cgtagccggc cttgaggaag
     3121 atctcgatgg aaggctggag cgggttgtag ccgagcgtgt tgatgaaatc ctcgccgagg
     3181 tctttctgca gctttttggc ggcgttctcg cgggaaacga attcagtgga cttgcgccag
     3241 ggttccgcat cgatggattt ctgcagcgcg atgatgtcga cgtccttcgc atcctggtcg
     3301 aggaaaacgg tgatcttgat gttctcgcgt acgtaatcgg aaacctcttt cgctttcagc
     3361 acgagcacgc tgagcaggcc gagcatgatg agcaccagcg agatgctgat cacgatcgtg
     3421 aacgaagaag aacggatgcg gcggcggctg taggtgtcgg acacgttttt taaactattg
     3481 cgcagcgaaa atacgagaaa gagaacgagc agtacgttcc caacgctcta atgtgggaaa
     3541 gtaagcccga acgtgaaaaa cttttcagga ggaggtgcgg accccgggac tttccatctg
     3601 aactgtgccg gtaaaatgac ggacttcacc acgcccggac aatgctgccc gctcccggtg
     3661 aaagcaaatg ccacggtgtc gcaccagact tttacggacg cgagtccctg aagagacata
     3721 aaccgttatc gaagctaccc gccggaaacg ttcaccgctg tttgttaccg ggtcaatctt
     3781 acgcctggcc ggttttgcaa gccgccggga gcggaaaggc ttatgactat atttgagatg
     3841 attgaggtaa tatgtaaatt ggcgacaacc cgcgacaaca gacacacata cattttcgga
     3901 atttaaaaaa taataatggc acaaaagaaa aaaagtgaag cagaactgac agcgactgag
     3961 gaagcaattg ttgcaaaaag taagttacct cttgtattca tcagtcacga cacgaaagac
     4021 gctgaaattg cagaggcatt cagcaaacta ttgagcagtg ttagtgctgg ggtattaaaa
     4081 tcatttcgtt cctcggataa gaaaggaact caagggattg aatacggagt tgagtggttc
     4141 ccggaaatta tgaaaaaact tgacgaagca tctgatatag tttgtttact tacacacaat
     4201 agcgtcgaca gaccttggat actttatgaa gcaggtgttg caaaagggaa attaagcacg
     4261 actgtgttag gtatcgcagt tggtattcca ttaaataaag caaacaacgg accttttgct
     4321 caattccaaa actgtgcaga cgatgaggac agtttaacta aattggtaat acaacttgta
     4381 aggcgaatac caaattcaga acctgacgaa gaaattatca aggaacaagt caaaacgttc
     4441 cgtgacaaac tttcgggaat gctaaagaaa cagccaaaag ttgaggagaa gaccaaagaa
     4501 ttaatggggg atgcctcaat cgcaaagttg tttgaagaag taaaaattat gtttcaagat
     4561 ttaccttcta gaatagacaa acgacttgac cctgaaatga taggaatgaa acggaggcgc
     4621 aggtttcatc caaaaatgat ggacgagctt ttccatatct ctatgaaagt aggcgacccc
     4681 aatatatcgt tcttgatgat gatttcatta gttaaagacg aaatgccttg gctttacgaa
     4741 attggaatag aaacttatag gtctttaaaa acagcaaaaa ccaagtctga caaacaaaaa
     4801 gccatttctc aatatcatca agctcttgag ttaatgggac atccaatttt cgttgagatg
     4861 tatgctcggt cggaagagag ttatatgatg atgaaagaaa tgagacactt aatgcatcgc
     4921 tactttgacc aatttttttt agatggcaaa ggatagagca gtcgccaaca cgacttttgc
     4981 accaataggc agacagtgga aataaaaacc cccaaggcaa aactgcagaa tgctgcaggc
     5041 agaattaaac gaccgctcaa ataagtgcaa gaaatgagtc aagacgaatt tagcgtgacg
     5101 atacacatgg tttcaagtct tgatggaatt attgccagaa aggacaatag catttcgtgg
     5161 tttgaaactt ctgacaaata cgacaaagga gttgacagtg aagatcccga agaatttcta
     5221 aaaacgatag actgctatgt aatgggatcg aaaacatacg aacttgcttt agagctttca
     5281 aaaaaatacg gctgggctta cggcgacaaa ccgacaaccg ttttaactaa ccgcaaactg
     5341 caaactaaca gacacaatat tgaattctat tcaggcgact taaacaaatt tgtgaatgaa
     5401 cgattaaaac caaaatttaa gaacgtatgg gttgttggcg gctctatgct taccaaagat
     5461 ttgattcggt taaaattagc agacgaaata cgagtaagca ttttgccaat catcctgggt
     5521 gacgggttat tatttttcga ccacattgga caggaacaag cgttacacct gaaagactcg
     5581 aaagcgtata aaaacggga
//
LOCUS       DASTSF010000536         2585 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_694282,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000536 DASTSF010000000
VERSION     DASTSF010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2585
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_694282"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..182
                     /locus_tag="VFU15_15300"
     CDS             <1..182
                     /locus_tag="VFU15_15300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719209.1"
                     /translation="GQCFHFGYDGDWNFEASIRNGVFTFLDNDATGGAPAMLSVPMYS
                     YPRYEELPVQLAQFE"
     gene            733..1476
                     /locus_tag="VFU15_15305"
     CDS             733..1476
                     /locus_tag="VFU15_15305"
                     /inference="COORDINATES: protein motif:HMM:NF012920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU4719210.1"
                     /translation="MTLAQRIKILRKHLGLSQTEFANEIGITQTSLSQIEGEKNGISY
                     DVFKGIIGKFRVSPAWLMDGEGEMLQTDEAARKGGALPLVVQVAGDEEENIVVVDKKA
                     AAGYLQGQQDPDFIAKLPSFRLPGFYGKTYRAFEITGDSMLPGIHPGDMLIGSYTESL
                     DHLRKGHIYIFVMHDGSIVAKRVSPLGENKFELISDNAGYDPYAVDAADIAQTWTVEA
                     RITKDLEKPGDDRISTLELRLKELEKKIN"
     gene            1502..1696
                     /locus_tag="VFU15_15310"
     CDS             1502..1696
                     /locus_tag="VFU15_15310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719211.1"
                     /translation="MQMNIYTTQLKKSIRQKLEHINDTEYLASLDAILTFETISPDGK
                     MPRQESTQKRSLLKKMFSLL"
     gene            complement(1771..2457)
                     /locus_tag="VFU15_15315"
     CDS             complement(1771..2457)
                     /locus_tag="VFU15_15315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719212.1"
                     /translation="MKNLLLFSFAVAFFFIRASAQNDNPDRPKTLIGDVHLFKNIHCD
                     YGMLDAGYLQGFIHGTMTRGFSWNVNGVMNKNWSTGLWMDLQESRHITPSLPELAVKP
                     DYVFGAFGWNNELLLMPNRVVNFSFPLHVGMGIASYNDRSVYTAVYPAQYGYYAQPKS
                     IGKGYYFVAEPGANMFVNLYKNVSLAIGASYRFVSGYSSIGGSADLSGLNMRATFRVR
                     LYDDAGNEKS"
BASE COUNT          670 a          628 c          689 g          598 t
ORIGIN      
        1 cggggcaatg cttccatttc gggtatgacg gggattggaa ttttgaagcg tcgatcagaa
       61 acggggtgtt tacgtttctt gataatgatg cgacgggcgg ggcccccgct atgctcagtg
      121 tgcccatgta cagctatccc cgttatgagg agctcccggt ccagcttgcg caattcgagt
      181 aaatcaaaga gcgatcaacg tcacttttaa aaatgcaaaa gccccgacct ctcgcggaaa
      241 cgaacaatag gtcggggaca aatgctgttg cagctttccc acaggcagaa agggaaactt
      301 ttgactcagg cgtaaaccat ggcgctaagc agctgcaaca ttgaagcctg tgttgccggg
      361 gcatgcggag gctttgctgc cgccggactg ttcacaggcc aggtagtgcg tggagcctga
      421 cgaattccgg ggttcagaac ccgtcaggga tttctccacg tcttgtgatg atgcgttcag
      481 gatgtttccg tccgcgggag gaattatgtt gccattaagg acgttaaagg gcatggcctt
      541 ctgaagcaag gcgctgatgt gaagtgtttc attcgttctg ttcgaaataa ccatttccgg
      601 tcctcccaaa gtttagaatt attaatttgt cctatatttg ctcttgttga agcaacaatc
      661 acagcacaaa aatatagctt aaattaatat ttacaaataa aaattattat ctttgcctaa
      721 taaatcaatc aaatgacact agcacagcga attaaaattc tcaggaagca tctcggcctc
      781 agccagaccg aattcgcaaa tgagatcggc attacacaga cgtctttatc gcagatcgaa
      841 ggcgaaaaga acgggatctc gtatgatgtc ttcaaaggca ttatcgggaa attcagggtg
      901 agtccggcgt ggctcatgga cggggaaggt gaaatgctgc agaccgacga agccgcaaga
      961 aaaggcgggg cgctgccgct cgtggtgcag gtggccggcg acgaggaaga gaacattgtc
     1021 gtcgtcgata aaaaagcagc ggcgggttac ctgcagggac agcaggaccc tgattttatc
     1081 gcgaagctcc cttcgttccg tcttcccggc ttttacggga aaacgtaccg cgctttcgag
     1141 atcaccggcg acagcatgct gcccggcatt catcccggcg atatgctgat cggctcgtat
     1201 actgaatcgc tcgatcacct gcggaaagga cacatttata ttttcgtcat gcacgacggc
     1261 agcatcgtgg ccaagcgtgt ttctccgctc ggtgaaaaca agttcgaact gatctcggat
     1321 aatgcgggtt acgatcctta tgcagtggac gctgcagaca tcgcgcaaac ctggaccgtg
     1381 gaagcgcgga tcacgaagga tctcgaaaag cccggcgacg atcggatctc caccctcgaa
     1441 ctgcgcctga aagagcttga aaaaaagatc aactgaccaa tgtagtattt atttaatgtc
     1501 tatgcagatg aacatttaca ccacacagct caaaaagagc atccgccaga aactcgagca
     1561 cattaatgat accgaatacc ttgcttcgct cgacgccatc ctcacgtttg agacgatctc
     1621 gcccgatggc aaaatgcccc ggcaggaaag cacgcagaaa agatcgctgc tgaaaaaaat
     1681 gttttcgctg ctgtaaaaaa aaatgccgca gcagctaagc gcggcatttt actgctgcat
     1741 cagggccgga ggctgtggcc gttggtgcat tcagcttttt tcattaccgg catcgtcgta
     1801 caggcgcacg cgaaacgtgg cgcgcatgtt caggcccgaa agatcggcgc ttcccccgat
     1861 ggatgaataa ccggacacaa aacggtagct cgcgccaatg gcgaggctca cgtttttgta
     1921 aagattgacg aacatattcg ctccgggctc cgccacgaag taataacctt tcccgatgct
     1981 tttcggttgt gcgtaatacc cgtattgcgc aggatacacg gccgtgtaaa cggacctgtc
     2041 gttgtagctg gcaatgccca tccccacgtg cagcgggaag gagaagttga ccacgcggtt
     2101 gggcatgagc agcagctcat tgttccaccc gaaagcgccg aaaacataat ccggcttcac
     2161 ggcaagttcg ggcagggaag gagtgatgtg ccgtgattcc tgcagatcca tccacaggcc
     2221 ggtcgaccag tttttattca ttacgccgtt cacgttccag ctgaagccgc gcgtcattgt
     2281 tccgtgaatg aagccctgca ggtagcctgc atcgagcatc ccgtaatcgc agtgaatgtt
     2341 tttgaaaaga tgcacgtcgc cgatgagcgt tttcggcctg tccgggttgt cgttttgggc
     2401 ggaagcgcga atgaagaaaa atgcgaccgc gaaggaaaag agtaggagat ttttcatgat
     2461 gattcttttt agtgaatgac tgaaactgaa aatttgattt ttccgtgcca ggtcgcagca
     2521 gggaacggat gatgaatttt acgcaaagac gggaaaaagg aatagcgttt tccgtcaatt
     2581 ggttc
//
LOCUS       DASTSF010000537         4052 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1664586,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000537 DASTSF010000000
VERSION     DASTSF010000537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4052
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1664586"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1948)
                     /locus_tag="VFU15_15320"
     CDS             complement(<1..1948)
                     /locus_tag="VFU15_15320"
                     /inference="COORDINATES: protein motif:HMM:NF017393.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M43 family zinc metalloprotease"
                     /protein_id="HEU4719213.1"
                     /translation="MKKKLSLLICALSLGGFAFAQAPYKGKILNGNQVPGNKVRCATD
                     EHETWLQQNDPSRAQARQDYEANIQSYIAAHPNEQNRSTITIPVVVHIVYYTGAPAQN
                     ISDNQVFSQIQVVNEDYGRTNSDVNTHWSQASGTNIQFCLAQRDPNGNATNGIERRGL
                     SSNVSFSTNDGVKHYSSNGLDAWDPTRYMNIWVCNLGQSLLGYGEFPTTSVSQTFGVV
                     ILYSAFGSNFTSYGTFSDIQSPYDRGRTTTHEFSHCFNLYHIWGDDNGSCSGSDNCSD
                     TPNQGNATTTCYSFPHTDNCSTSSPGIMFENYMDYSYDNCLNLFTAGQSARMNAVLSS
                     APYNALATSNGCQPLTTTPLDAGISSITTPANNATYCSGTFTPVVVLKNFGSSTLTSC
                     VINYKIDNNTVQTYNWSGSLATNATANVTLSSQTATNGTHTFTCYTSSPNGGTDGQTS
                     NDQSQNTFTVSGTGQSLPYTQGFESTTFVPTGWTLNNPDGSNTWARTTTAHTSGSACA
                     FMDNWSYQTGNGQIDEMTMPALNLSSVSNPVMTFQVAYTYWTVPYQYSDTLKIYISTD
                     CGATWTQIYNKYGSNLQTAPPVSSTTSPFTPSSTQWRLETISLLPYQSAGSAMIKFRN
                     ISDYEDDLYLDDINITNTTDVPTFN"
     gene            2133..2825
                     /locus_tag="VFU15_15325"
     CDS             2133..2825
                     /locus_tag="VFU15_15325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU4719214.1"
                     /translation="MKRSILLVEDEENLLKAIRLNLELEGYEVIPAIDGESAVTIFRS
                     SAVDLVILDVMLPLLNGFDVCEKIRETDREVPVLFLTAKAQGEDKVRGLRLGADDYIT
                     KPFNLEEFLLRVNNLLRRSPKKHLAQEMLQEFRFGNNLVNFSTYEASNAVGSLQLSKR
                     EAELLKLLISRNGQVVSRGEILELLWKNEESPTGRAIDNYILNFRKYFEENPRAPRFF
                     HSIRGVGYRFTG"
     gene            complement(2829..3161)
                     /locus_tag="VFU15_15330"
     CDS             complement(2829..3161)
                     /locus_tag="VFU15_15330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719215.1"
                     /translation="MSKYKFNLDPKEPREEIVRKHKDFGKVMQNYQKMTKPLYRTPLY
                     RFRRIFMVVIAVLAVSWLVSEFGDFDRNEKNSPQKPDTMQHKTGNDSPVIRVQPMIRN
                     DSLKDFKK"
     gene            complement(3164..3733)
                     /locus_tag="VFU15_15335"
     CDS             complement(3164..3733)
                     /locus_tag="VFU15_15335"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU4719216.1"
                     /translation="MNGHHKTDEALREEQQQVESAKADPSKFGVLYEKYYRQIFVFVF
                     RRTNDDEIAGDIVSNTFLKAMLSLKSYTFKGVPFSAWLFRIALNEVNLFFRKTKRERV
                     VSLDRSGIVSVIAESGQEDSEGHRILLMKILGTLPAEEMQLIELRFFEKRPFSEVGEI
                     LGITENNAKVKTYRILDKMKQTFNRFAER"
BASE COUNT          863 a          995 c         1161 g         1033 t
ORIGIN      
        1 ggttgaacgt cggaacatcc gttgtattcg tgatgttgat gtcatccagg tacaggtcgt
       61 cttcgtaatc gctgatgttg cggaacttga tcattgcgct tcctgcagat tgatacggca
      121 gcagcgaaat ggtttcaagt ctccattgcg tggacgacgg ggtgaacggc gacgttgtgc
      181 tggaaaccgg cggagccgtc tgcaggttcg agccgtactt gttgtagatc tgcgtccatg
      241 tagcaccgca gtcggtagag atgtagatct tcagcgtgtc cgaatattgg taaggaacgg
      301 tccagtaagt ataggctacc tggaaggtca tcacggggtt ggacaccgat gaaagattga
      361 gtgccggcat ggtcatctcg tcgatctgcc cgttaccggt ctggtagctc cagttgtcca
      421 tgaacgcaca ggcgctgccg cttgtatgcg cggtggttgt acgcgcccat gtattgctgc
      481 cgtcaggatt gttcagcgtc cagccggtgg gaacaaaggt ggtgctttca aatccctgcg
      541 tgtacggcag gctctggccc gttccgctga ccgtgaatgt attctgcgac tggtcattgg
      601 aggtctggcc atccgttccg ccgttcgggc ttgacgtgta gcacgtgaac gtatgcgttc
      661 cgttagttgc cgtctgcgat gagagggtaa cgttcgctgt agcgttagtg gcaaggcttc
      721 cgctccagtt gtatgtctga actgtattgt tgtcgatctt gtaattgatc acgcagcttg
      781 tcagcgtgct gcttccgaaa ttcttgagaa caaccaccgg ggtgaaagtt cccgagcaat
      841 aggttgcgtt gttagcagga gtggtgatgg aagagatgcc tgcatcgagc ggcgttgtcg
      901 tcagcggctg gcagccgttc gacgttgcga gcgcattgta aggagctgag ctgagtaccg
      961 cattcatgcg tgcagactga cctgccgtga aaaggttaag gcagttgtcg tagctgtagt
     1021 ccatgtaatt ttcgaacatg atgcccgggc ttgaggtcga gcagttgtcc gtgtgcggga
     1081 acgaatagca cgtcgtggtt gcattaccct ggttcggtgt atccgagcaa ttgtcagatc
     1141 cggagcagga gccattgtcg tcaccccaga tgtggtagag gttgaagcaa tgcgagaact
     1201 cgtgcgtcgt ggtgcgtccg cggtcatacg gtgactggat gtcagagaag gtgccgtagg
     1261 acgtgaaatt gctgccgaag gcagaataca ggatgacgac gccgaatgtc tgcgacacag
     1321 aggtggtcgg gaattccccg taaccgagca ggctttgccc gaggttgcag acccagatgt
     1381 tcatgtaacg cgtgggatcc catgcgtcga gcccgttgga gctgtaatgt ttcacgccat
     1441 cattcgtgga gaaagaaaca ttcgagctca gtccgcggcg ttcgatacca ttggtggcat
     1501 tgccattggg atcgcgctgc gccaggcaga actggatgtt ggtcccggaa gcctgcgacc
     1561 agtgcgtgtt aacgtctgag ttcgttcttc cgtaatcttc attcaccacc tggatctgtg
     1621 aaaagacctg gttgtcggaa atattctgcg ccggtgcacc tgtgtaatac acgatgtgga
     1681 caacgacggg aatggtaatg gtcgaacggt tctgctcgtt cgggtgtgcg gcaatgtatg
     1741 actggatatt ggcttcgtaa tcctggcgtg cctgtgcacg ggacggatca ttctgctgca
     1801 gccatgtttc gtgttcgtcc gtcgcgcagc gaactttatt cccgggaacc tggttcccgt
     1861 tcagaatttt tcctttgtag ggtgcctgag cgaaggcaaa accgccaagg gaaagcgcgc
     1921 agatgagaag agataacttc tttttcatgc agtttttttg tttggtgtgc ttgattcttg
     1981 tttgtttgat gtttgggcga tcgtgaagat gcaaaaaaat atttcaaatc acaaggctgg
     2041 ttaaaattat cagcttcatt atctgtgagt tagtcgctgg aaaaacaggg tttaccgcag
     2101 tcaaatgctg acacggccta attttaccgg aaatgaagcg aagcatcctg cttgtcgagg
     2161 acgaagagaa tctcctgaaa gcgatccggc tcaacctgga gctcgaagga tacgaggtca
     2221 ttcccgcgat tgacggagaa agcgcggtga ccattttccg gtcgtccgct gttgatctcg
     2281 tgatcctcga cgtgatgctt ccgctgctca acggtttcga cgtctgcgaa aagatcaggg
     2341 aaacggaccg cgaagtgccg gtgctttttc tcaccgcgaa agcgcagggg gaagacaagg
     2401 tgcgcggact tcgtctcggt gcagatgatt acatcacgaa gccgttcaac ctcgaagaat
     2461 tcctgctgcg cgtgaacaac ctgctgcggc gttcaccgaa aaaacacctg gcgcaggaga
     2521 tgctgcagga attccgtttc gggaacaacc ttgtgaattt ttccacgtac gaagcatcga
     2581 acgcggtggg aagcctgcag ctttccaagc gggaagcgga gctgctgaag ctgctgatca
     2641 gccgcaacgg ccaggtggtt tcgcgcggcg agatactcga gctgctctgg aagaacgagg
     2701 aatcgcccac cgggcgcgcc atcgacaatt acattctcaa tttcaggaaa tacttcgagg
     2761 aaaatccacg cgcgccccgg ttctttcatt ccatacgcgg ggtgggctac cgtttcaccg
     2821 gctgagcgtt attttttgaa atccttcagc gaatcattgc ggatcatcgg ctgtacgcgg
     2881 atcacgggag aatcgttccc ggttttatgt tgcatcgtat ccggtttttg cgggctgttt
     2941 ttttcgttgc ggtcgaaatc accgaactcg gaaaccagcc aggaaacggc gaggacagcg
     3001 atcaccacca tgaagatgcg gcggaaacgg tagagcggcg tgcggtacag cggcttcgtc
     3061 atcttctggt aattctgcat caccttgccg aagtccttat gtttccgtac gatctcctcg
     3121 cgcggctctt tcggatcgag attgaattta tacttgctca ttttcagcgt tccgcaaagc
     3181 ggttaaaagt ttgtttcatc ttgtcgagta tgcggtaggt cttcaccttc gcattgtttt
     3241 ccgtgatgcc gaggatctct ccgacttctg aaaaaggacg cttctcgaaa aaacgaagct
     3301 cgatgagctg catttcttcc gcgggcagcg tgccgaggat cttcatcagc aggatgcggt
     3361 ggccttcgct gtcttcctgc ccgctttcgg cgatgacgga aacgatcccg gatctgtcga
     3421 ggctcaccac gcgttctctt tttgttttac ggaagaacag gttcacctcg ttcagcgcga
     3481 tccggaacag ccaggctgag aacggaactc ctttgaacgt atagcttttc agcgagagca
     3541 tggccttcag gaacgtgttg gaaacaatgt cgcctgcgat ctcatcgtcg ttcgtgcggc
     3601 ggaaaacgaa cacgaagatc tgccggtaat atttttcgta gagcacgccg aacttcgacg
     3661 gatctgcttt cgccgattcc acctgctgct gctcttcgcg cagcgcctcg tccgttttgt
     3721 gatgcccgtt caagcttctt tttggcttcc taatgcaaag atgagggaaa agatacagcc
     3781 gcaacctctt gttgttacca ggcggcatgc tgcgcggaat tcattgccgg atgaaacttt
     3841 gcggaacccg gggcaaccgt ctgtaactaa tttgcgtctt gaggttatac atacggctaa
     3901 acaattgaaa catgcgatca gtaaccctac ttctgcttgc cggattattt ccgctttcct
     3961 cgttcgctgc caaaggaata aaagaatcgc tgcagcatcc tgaacatacc acgggcctcg
     4021 tgcttacttc gtcaccggtc gaagccagcc tg
//
LOCUS       DASTSF010000538        11860 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_365570,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000538 DASTSF010000000
VERSION     DASTSF010000538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 11860)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11860)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11860
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_365570"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1430
                     /locus_tag="VFU15_15340"
     CDS             <1..1430
                     /locus_tag="VFU15_15340"
                     /inference="COORDINATES: protein motif:HMM:NF020337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HEU4719217.1"
                     /translation="NGGAHIRDAYVHTLSQDGNLHLDVRTYEPCILYVNGQYWGVYEI
                     REKVDDLDYTSYYYDQDDPNVQMLKTWGGTWSKYGGPQAQTDWNTFESFVVSNNMALP
                     ANWAYVDSVYNWKSLCDYIILNSICVTSDWLNWNTQWWRGLDTAGHAKRWRYCLWDND
                     ATFGHYINYTGIPDTSPAADPCNPQSLPDPGGQGHIPVLDALMNNPIFYQYYVNRYAD
                     LLNTTFSCDTMVTLLDTLTNRIAPEMTGQCTRWGGSYTGWQTNVNTMRTYIQTRCTAI
                     QQGMIDCYSLTGPYNLTVDVVPAGAGTVNVNSIHLASSQLPWTGLYYGGINIILQTAP
                     TSSNYVFDYWELVNTPSPDSLSDSITVNLASADHIVAHYHMIDNPPPTQTGSLFIPTA
                     FSPNADGNNDVLHMHGTEGASYIDLVIYNRWGQKVFETTDPTQGWDGTTGGRECESGV
                     YAYFLKVDYPDGTSTTKSGNITLMR"
     gene            1440..2465
                     /locus_tag="VFU15_15345"
     CDS             1440..2465
                     /locus_tag="VFU15_15345"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HEU4719218.1"
                     /translation="MKKIVILIFSGVFSFAAKGQDIHFSQFNETPMHLNPAYTGMFDG
                     LFRITLNYRQQWAAMGHPYTTAAAAFDMPLMYAKDRAYMGIGAFLYRDQAGDSKFGTF
                     TGALSFSGIVPLSENDKLSAGMMGGYSQKSATITSLQWENQYVNGAYDPTAATGESNL
                     LTSFPYADLAAGFAYQHRHEIGNIAEKNIFELNAGASAFHFNRPSEKFRGGGGEKLDM
                     RYVFHAQVRYDLPGTRFSVRPSAYYMSQGPAHEIVFGTLLRYRIRNGTKITNFFSESG
                     IGLGVHYRWNDAIIPQLYYDLGDMFIGLSYDLNISRYSTVSKMNGGFEVTIRYADLNS
                     ALYKNKK"
     gene            2602..3126
                     /locus_tag="VFU15_15350"
     CDS             2602..3126
                     /locus_tag="VFU15_15350"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU4719219.1"
                     /translation="MNEISLVPAGKNDMQDIASLAETIWKTHYTPIIGEKQVEYMLGL
                     MYSGEGMKKQIDEGQQFFFVKENGRNAGYISVSDKGNGEFFLHKFYIAVSQQGKGLGK
                     KVFALILQQFPSLASLTLTVNRKNYKSVNFYFRLGFVISEVKDFDIGNGFFMEDFVMN
                     YKRAGTRPPVPPQE"
     gene            3123..3971
                     /gene="prmA"
                     /locus_tag="VFU15_15355"
     CDS             3123..3971
                     /gene="prmA"
                     /locus_tag="VFU15_15355"
                     /inference="COORDINATES: protein motif:HMM:NF001785.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006479 - protein methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEU4719220.1"
                     /translation="MNYTELTFTVQPLQPGCDLLISELADRGFESFSETETGFAAYIP
                     ESLFSVEQLADLEKFRSEKLDFHYTVKHIPSQNWNEEWERNFSPVEIAGQLRIRAPFH
                     APDASFPSEIVIQPRQSFGTGHHATTRLMIQKLLTMAFPGRYILDMGCGTGVLAIFCA
                     QHGAAGILGMDIDPGSVENAGENALLNGVNNINFETGTETEIGDRKFDLLLANINKNV
                     LKNAMRTYAGSLRAGGEILLSGFFTTDVPELIAAAEAEGLHPVRVESEQEWALVHLAK
                     KNRNLL"
     gene            3968..8545
                     /locus_tag="VFU15_15360"
     CDS             3968..8545
                     /locus_tag="VFU15_15360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719221.1"
                     /translation="MKEKMRIVLLCAVATFVSARGLFAQKMPASFTADNVKFIDEMKD
                     FFESYDGKAGRDFIDDFNQNYWMNGKVSDDIKQAMFKNTNQMCKKKFRPSPEYYSYFN
                     TIKAIVDKNFPMTTFLDWQSCFTSIGNGKLGKPFSDYILMSESLFGENKFYKTATGEW
                     SVTGGTWKFACDSVPSVRFSDVTLKGNAKNDSTRINGTSGTYYPTNGMWIGKGGRIDW
                     QRAGLKEDDAYADLKNYSFPVKGFVFIADSVTFYNKKYFKDKPLTGRLTEKLVADAKA
                     STAAYPRFESYSSRYQIKSMYPNVDYEGGFTQVGAKFIGSGTPGNPAFFIFKRNGKNF
                     LQVTSQSFAITDQKVTSQNSAIKFSFDADSIVHPSVDFKYFVDSSRVVLYRPDEGASR
                     APYYDSFHKVDMFVEQIIWKTNEPTIVLNTVPGSTVGEASFPSVDYYRQYLYDQLQGI
                     EPVHPLIKIRNFVRDVNGGVKTFTMMDLAKYWKISVEELRPTIMNLSNQGFLLYDPVT
                     DMITYKDKCDTYIAARAGKKDYDNIIFESKVQPGTPNAKINLLNYDMTLFGVKEIGLS
                     DSQNVVVFPNQDMVVLKKNRNFTFEGSIMAGRFDYYGKLFSFDYNTFKLSLNNVDSVR
                     IWVDTPQRDPRDPKGGFVQMKVRSVIENLNGELQIDASDNKSGVKNKGPYAKYPIFTS
                     AKPAFVYYDKPGIQKGVYNRDKFYFKLDPFTIDSLDNFQNSALHFVGTFQSSGIFPEI
                     RDTLRLMPDYSLGFVRQAPPGGYPVYGGKAKFTNEMRLSNQGLRGDGQIDYITSTSIS
                     KDFIFFPDSMNGVAQSFTMKEQKIQGKTEYPQVNGPGAYIHYMPKKDYLNATSRDSAF
                     SVFNGKSTFTGTMTLSPKSLEGNGKLDFSNAELTSKKMVLKQHVADADTADFDLKALA
                     LAGLAFSTKNVNAHVDFEKREGDFKANGKGSIVTFPVNQYICYMENFKWYMDQDQIEM
                     SSNKPKPGEAQDKIALQGPEFISIHPKQDSLRFRAPKAKYDLKNYIIHAEGVQEILVA
                     DARIIPDSGNVVIQKGAYMETLTNAQLQANTITNYHKLFNCVIDVYSRHSYTASGDYA
                     YVDELKKEQIIHFAKIAPDTTGQTYAQGTIADTSKFYLSPAFDFRGNVILAANNQFLV
                     FDGSTSLTNDCGIDKARLQFKGEINPDEIYIPVSDNPVTDKGDPITSGLMSTVSADSV
                     HIYGSFLSPKKGKTDVSVVSADGFLFFDKTSREYRISNKEKLVERSLPGNYVSFNTKN
                     CTIYGEGRMALGADLGKVLLTPIGNATHNTVDHSTSFDLVMGIDFFIPEKLLNYVLDD
                     IDASTSLAGTDPTRTTFQHALTEMVGKERADKLISNLTVFNSYKKFPDELKYTFFFTD
                     VQMNWNQKTKAYISDGKLGLGNSDKKEINKFVPGTIMLERVRSGDRLTIYFELDNGKW
                     YTFYYANGMMSAYSSNTKFMDELKAMKDDDKVAPDDYDKDRLSEKGQKAKYKITAGGP
                     QDLSRLKNKLKKASDGGDDSGGGDGDDSGNK"
     gene            8546..9214
                     /gene="plsY"
                     /locus_tag="VFU15_15365"
     CDS             8546..9214
                     /gene="plsY"
                     /locus_tag="VFU15_15365"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013666256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEU4719222.1"
                     /translation="MTTTSIIALLIAYLLGSIPSAVWIGKLFFGTDVREHGSGNAGAT
                     NTFRVLGKKAGIPVLLLDILKGFLAVNLAWLVTEAPPHTSDFVDLQIVLGVAAVLGHI
                     FPVFAGFRGGKGIATLFGVTLAVHPQTCLLATGVFLLVFLSTNYVSLGSISAGLSMPL
                     FMMFFFASEAFPSMIAYCVMVSMLILITHQKNIERLLRKEESKMRLTRRKNSGEDASD
                     AGVK"
     gene            9477..>11860
                     /locus_tag="VFU15_15370"
     CDS             9477..>11860
                     /locus_tag="VFU15_15370"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEU4719223.1"
                     /translation="MTALAVLLRPFPVFSQAPGSEEDVIRNADAAFDKADYAKAMPLY
                     SQLLANHPADPNYNYKYGVCVLYASSEKDKALPFLEKASADPKADADVWFYLGKAYHL
                     NYQFDQAIVAYTKYKQLVGEKKAEKMQVDNQIAMCKTGRKLLKAITDITVLEKAELNE
                     QDFYKHYDLKSAGYSGQLLAEPDEFKTALDKKKGVTSIIYLSGEKSELYFSSYGDDES
                     QGKDIYVVRRLPNQKWGKPQNVGYPINTEYDEDYPFLMPDGKVLYFSSKGHNSMGGYD
                     VFRAELNEETNTWEKPVNLDFAINSPDDDIMFVSDANEQTAWFASNRNSPEGKMTVYH
                     VVIQRKPVQNCMISGTFVSTDGDPNEKAKITVKNEDTNEPIGVYTSNQSSGSYFMNLP
                     NNGGKYSFTVEHSGIHTQTQTVIIPPQYEIKTVNQRIHYVDVNGDQQLVIETDFSSDT
                     STLDPLFLKDKAKLDVNSGPDAYQVVDMGNPDNAGKNPGNTTDPDNSNVTDPGDDPSD
                     SIDPADSVPSSNAGKVTDAELVKSAYDDANATKAEAEDMQNQANNAYAYAADLDRQAK
                     AMQDSATNAKKAADAMADGPDKVAATKRADDLQAQANSLQSQTVAAFNVAQAMDTDAQ
                     NKKNEADKTQQYAASLDKAVKSNDPKALDQAQKQADDLQQYSVTTTQKDQTVQNISKL
                     DDQKHADLKKAQDDLDLMQADNKDNYDRIAQLQKDKDKEKDKDLRAGYDDQIQGLKDD
                     IDDNNAKIAKQQAYVNSLQNDVNDLDNQLHGVTQAAAQPKTNTGAVVSPQQKKDL"
BASE COUNT         3104 a         3058 c         3128 g         2570 t
ORIGIN      
        1 agaacggggg cgcgcacatc cgcgacgcat acgtgcatac actttcgcag gatggaaatc
       61 ttcatctcga cgtaagaact tacgagccct gcatcctcta tgtgaacgga caatactggg
      121 gcgtttacga gatccgtgaa aaagtggacg acctggatta tacatcctat tactatgacc
      181 aggacgatcc gaatgtgcag atgctgaaaa cctggggagg cacatggtcg aaatacggcg
      241 gaccacaggc gcagaccgac tggaacacgt ttgagtcgtt tgtcgtttcg aacaacatgg
      301 cgcttccggc aaactgggcc tacgttgatt cggtctataa ctggaaaagt ctttgtgatt
      361 atataatact caattccatt tgcgttactt ctgactggct gaactggaac acacaatggt
      421 ggcgcggact ggacaccgcc ggtcatgcca aacgatggcg ctattgcctg tgggacaatg
      481 atgcgacatt cggacattac atcaattaca cgggcatccc ggacacatct cccgccgcag
      541 atccgtgtaa cccgcagtct cttcctgatc ccggggggca gggccatatc cccgtgctgg
      601 atgcgctgat gaacaacccc atattttacc agtattatgt caaccggtac gccgacctgc
      661 tgaatacgac tttcagctgc gatacgatgg tcaccctgct cgatacgctc accaacagga
      721 tcgccccgga aatgacggga cagtgcaccc gctggggcgg cagctatacc ggctggcaga
      781 ccaacgtgaa taccatgcgc acgtatatcc agacacgctg cacggctatc cagcagggaa
      841 tgatcgattg ctattcgctg acaggaccgt acaatctcac ggtggacgtt gttcctgcgg
      901 gggccggaac cgttaatgta aattccattc accttgcatc aagccagctg ccctggacgg
      961 gactttatta cggcggaata aatattattc ttcagaccgc gccgacgtcg tccaactacg
     1021 ttttcgatta ctgggaactg gtgaatacgc cttccccgga ttcgctcagc gacagcatca
     1081 cggtcaacct ggcttccgcc gatcacatcg ttgcgcatta ccacatgatc gataacccgc
     1141 cgccaacgca gacgggaagc ctttttattc ccacggcctt ttctccgaat gcagatggta
     1201 ataacgacgt gctgcacatg cacggcaccg aaggagcgtc gtatatcgat ctggttattt
     1261 acaaccgctg gggacagaaa gttttcgaaa ccacggatcc cacgcaggga tgggacggga
     1321 cgacgggagg ccgggaatgt gaatccggtg tttatgcgta tttcctgaaa gtggattatc
     1381 ccgacggtac gagcaccacg aagtcaggaa atattacgtt gatgcgataa acgcgcggca
     1441 tgaaaaaaat tgtcatcctt attttttcag gtgttttttc tttcgcagcg aagggacagg
     1501 atatccattt ttcgcagttc aatgaaacgc ccatgcatct caacccggcc tacaccggta
     1561 tgttcgacgg gcttttccgc atcacgctga actaccgcca gcaatgggcg gcgatggggc
     1621 atccgtacac gacagcggcc gctgctttcg atatgccgct gatgtatgcg aaagaccggg
     1681 cgtacatggg catcggtgcg ttcctgtacc gcgaccaggc cggcgattcg aagttcggaa
     1741 cgttcacggg ggcgctttcc ttcagcggga tcgttccgct cagcgaaaat gacaagctgt
     1801 ccgcaggcat gatgggcggc tattcgcaga aatcggcgac gatcacctcg ctgcaatggg
     1861 aaaaccagta cgtgaatggc gcctacgatc cgacggccgc cacaggggaa agcaacctgc
     1921 tcacgtcctt tccgtacgca gatcttgccg cgggctttgc ctaccagcat cgtcacgaga
     1981 tcgggaacat cgctgaaaaa aatattttcg agctcaatgc gggagcttcc gcatttcatt
     2041 tcaaccggcc ctccgaaaaa ttccgcggcg gcggtggcga gaagctcgac atgcgttacg
     2101 tattccacgc ccaggtcagg tatgacctcc cgggtacccg cttttcggtt cgtccttccg
     2161 cgtattacat gtcgcaggga ccggcgcacg aaatcgtttt cggaacgctg ttgcgttacc
     2221 gtatcaggaa cggaaccaag atcacgaatt tcttttcgga atcgggaata ggcctcggcg
     2281 tgcattaccg ctggaatgac gcgatcattc cccagctgta ttacgatctc ggggatatgt
     2341 tcatcgggct ttcgtatgat cttaacatct cgcgttattc caccgtttcc aaaatgaacg
     2401 gcggtttcga ggtgacgatc cgctatgccg acctgaacag cgcgctgtac aaaaacaaaa
     2461 agtaaatttg cggcgtatga aagagcgatt ccgggtgttc tacaaaaaat acctgacaca
     2521 atttcccgac tttttccagt atattctcct gatcgtgctt tcgatcgttg cctggatttt
     2581 tttctggtaa tttcgttccc catgaatgaa ataagcctcg ttcccgcagg gaagaacgac
     2641 atgcaggaca ttgcatcact cgcggaaacc atctggaaaa cgcattacac acccatcatc
     2701 ggcgaaaaac aggtggagta catgcttggt ctcatgtatt ccggggaagg gatgaaaaag
     2761 cagatcgatg aaggacagca gttttttttc gtaaaggaaa acggcaggaa tgcgggatac
     2821 atttccgtca gcgacaaagg caacggggaa tttttcctgc acaagtttta catagccgtt
     2881 tcgcaacagg gaaaaggact ggggaaaaaa gtgttcgcgc tgatccttca gcagttccct
     2941 tcactggcaa gcctgacgct gacggtgaac cggaaaaatt ataaatccgt taacttttat
     3001 ttcaggctgg gtttcgtgat ttcggaagta aaggatttcg atatcgggaa cgggtttttt
     3061 atggaagatt ttgtcatgaa ctataagcgc gccggaaccc ggccgccagt cccgccacag
     3121 gaatgaatta cactgagctg acatttaccg tgcaaccgct tcagcccggg tgcgacctgc
     3181 ttatttcaga acttgcagat cgcggattcg aaagcttttc ggaaacagaa acgggatttg
     3241 ccgcatacat ccctgaatcg cttttttcgg tggagcaact ggctgatctt gaaaaattcc
     3301 ggtcggaaaa actggatttc cattatacgg tgaaacatat cccgtcgcag aactggaacg
     3361 aagaatggga gaggaatttt tcgcccgttg aaatagcagg ccagctccgt atccgtgcac
     3421 cgtttcatgc gcccgacgcg tctttccctt cggagatcgt catccagccc cgtcaatcct
     3481 tcggcaccgg tcaccacgct acgacgcgat taatgattca aaagttatta acaatggcat
     3541 ttcccggacg ttatatactc gacatgggtt gcggtaccgg tgttcttgcc attttctgcg
     3601 cgcagcatgg cgctgccggg attctcggta tggatattga tcccggatct gtggaaaacg
     3661 cgggggaaaa tgccctactg aatggggtga ataacattaa tttcgaaacg ggcacagaga
     3721 cggagatcgg ggatcggaaa ttcgatcttc tcctggccaa catcaataaa aacgtgttga
     3781 agaacgccat gcgaacgtac gccggaagcc ttcgcgcggg aggagagatc cttctcagcg
     3841 gtttttttac caccgatgtt cccgagctga tcgcagcggc ggaggcggag gggctgcatc
     3901 ccgtacgagt cgaatctgaa caggagtggg cgctcgtcca ccttgcaaaa aagaaccgga
     3961 acctgctatg aaagagaaaa tgaggattgt cctgctgtgc gcggtggcca cttttgtttc
     4021 cgcccgcgga ctgtttgcac agaaaatgcc ggccagcttt actgccgaca acgtcaagtt
     4081 cattgacgag atgaaggatt ttttcgaaag ctacgacggg aaggccggcc gggatttcat
     4141 cgacgatttc aaccagaatt actggatgaa cggaaaggtg agcgatgaca tcaagcaggc
     4201 gatgttcaag aacaccaacc agatgtgcaa gaaaaaattc cgcccgtcgc cggaatatta
     4261 ttcctacttc aacaccatca aggcgatcgt ggacaagaat tttcccatga ccactttcct
     4321 cgactggcag tcgtgcttta cgtccatcgg gaacggtaag ctgggcaaac ctttttccga
     4381 ttacatcctg atgagcgaaa gccttttcgg ggaaaataaa ttttacaaaa cggcgaccgg
     4441 cgaatggagc gtaaccggcg gaacatggaa gtttgcctgc gacagcgttc cttccgtgcg
     4501 tttttccgat gtaacgctca aaggaaatgc gaagaacgac agcacgcgca tcaacggaac
     4561 ttccgggacg tattatccca cgaacggaat gtggataggc aagggcggcc gcatcgactg
     4621 gcagagagcc ggactgaagg aagacgacgc ctatgccgac ctgaagaatt attctttccc
     4681 ggtgaaaggt ttcgtgttta ttgccgattc cgtgacgttc tacaacaaga aatatttcaa
     4741 ggacaaaccc ctgaccggaa ggctgaccga aaagctcgtg gccgatgcga aagccagcac
     4801 ggcggcgtac ccgcgtttcg aatcgtacag ctcgcgctac cagatcaagt ccatgtaccc
     4861 taacgtggat tacgaaggtg gatttacgca ggtgggtgcg aagttcatcg gttccggcac
     4921 gccgggaaat cctgcttttt tcattttcaa gcgcaacggg aaaaacttcc tgcaggtcac
     4981 ctcgcagagc ttcgccatca ccgatcagaa agtgacgagc cagaattcgg cgatcaagtt
     5041 ttccttcgac gccgattcca tcgttcatcc ttccgtcgat ttcaaatatt tcgtcgacag
     5101 ctcacgcgtg gttttgtacc ggccggatga gggcgcttcc cgcgcgccgt attacgattc
     5161 gttccacaaa gtggacatgt tcgtcgagca gatcatctgg aaaacgaatg agcccacgat
     5221 cgttctcaat acggttcccg gatccacggt gggcgaagcg agctttcctt ccgtcgatta
     5281 ttaccgccag tacctctacg accagctcca gggaatagaa ccggtgcacc cgctgatcaa
     5341 gatccggaat ttcgtgcgcg acgtgaacgg cggagtgaaa acatttacga tgatggacct
     5401 ggcgaagtac tggaagatct cggtcgaaga attgcgcccc acgatcatga acctgtccaa
     5461 ccagggcttt ctcctgtatg atcctgtgac ggacatgatc acgtacaagg acaagtgcga
     5521 cacgtacatc gctgcgcgtg ccgggaagaa ggattacgac aatattattt tcgaatcgaa
     5581 agtgcagccg ggaacaccga acgcgaagat caacctgctc aattacgaca tgacgctttt
     5641 cggcgtgaag gagatcggtc tcagcgattc acagaacgtg gtcgtgtttc ccaaccagga
     5701 tatggtcgtg ctgaagaaga acaggaactt cacgttcgaa ggttccatca tggcgggccg
     5761 tttcgattat tacggaaaat tattttcgtt tgattacaat acgttcaagc tttcgctgaa
     5821 caacgtggac tccgtgcgta tctgggtgga tacgccgcag cgtgatcccc gcgacccgaa
     5881 aggaggtttt gtgcagatga aagtgcggag cgtgatagaa aacctgaacg gggaattgca
     5941 gatagacgcg tcggataata aatcgggcgt gaagaacaag ggaccgtatg ccaagtaccc
     6001 gatcttcacg tcggcgaaac ccgcgttcgt gtattacgac aagccgggca tccagaaagg
     6061 cgtttacaac cgcgataaat tttatttcaa gctcgatccg ttcacgatcg acagcctcga
     6121 taacttccag aacagtgcgc tgcattttgt gggaacgttc cagtcgtcgg gcattttccc
     6181 ggaaatacgc gatacgttgc ggctcatgcc ggattattcg ctcgggttcg tgcggcaggc
     6241 gccgccgggc ggttatcctg tttacggcgg caaagctaaa ttcaccaatg aaatgcggct
     6301 gagcaaccag ggactgcgcg gtgacgggca gatcgattac atcacttcaa cttcgatctc
     6361 gaaagatttt atctttttcc ctgattcgat gaacggtgtg gcccagtcct tcacaatgaa
     6421 agaacagaag atccagggca aaacggaata cccgcaggtg aatggccccg gcgcatacat
     6481 ccactacatg ccgaagaagg attacctcaa tgcgaccagc cgcgattcgg ctttcagcgt
     6541 gttcaacggg aaatccacgt tcaccgggac gatgacgctt tccccgaaaa gcctcgaagg
     6601 aaacggcaag ctcgattttt ccaatgccga gctgacgtcg aaaaaaatgg tgctcaagca
     6661 gcatgtcgcc gatgcggata cggcggattt cgatctgaag gcgctcgcgc tggccgggct
     6721 cgccttctcg acgaagaacg tgaacgcaca tgtcgacttt gaaaaacgcg aaggtgattt
     6781 caaggcgaac ggaaaaggtt ccatcgtcac gttcccggtc aaccagtaca tctgctacat
     6841 ggagaatttc aaatggtaca tggaccagga ccagatcgaa atgagcagca acaagcccaa
     6901 gccgggcgag gcacaggaca agatcgcgct gcagggaccc gaattcattt ccattcaccc
     6961 gaaacaggat tcacttcgtt tccgtgcgcc gaaagcaaaa tacgacctga agaattacat
     7021 catccacgcc gaaggcgtgc aggaaatcct tgtggcggat gcacgcatta tcccggacag
     7081 cggaaatgtc gtcatccaga aaggcgcata catggagacg ctcacgaacg cacagctgca
     7141 ggcgaacacg atcacgaatt accacaagct gttcaactgc gtcattgatg tgtattcccg
     7201 ccacagctat acggcttcgg gcgattatgc gtacgtggat gagctcaaaa aagagcagat
     7261 catccatttc gcgaaaattg cgccggacac caccgggcag acctacgcgc agggcaccat
     7321 tgccgatacg tcgaaattct atctcagtcc ggcattcgat ttccgcggca acgtcattct
     7381 cgcagcgaac aaccagttcc tcgtttttga cggatcgaca tcgctcacga acgactgcgg
     7441 catcgacaaa gcgcgactgc agttcaaagg cgagatcaac ccggatgaaa tttacattcc
     7501 ggtttccgat aacccggtta cagacaaagg cgatccgatc accagcgggc tgatgagtac
     7561 ggtctccgcc gactcggttc acatttacgg ttcattcctt tcgccgaaaa aggggaaaac
     7621 cgatgtcagc gtcgtgagcg cggacggatt tttattcttc gacaagacgt cacgtgaata
     7681 ccgcatttcg aacaaggaaa agctcgtgga acggtcgctg ccgggaaatt acgtcagctt
     7741 caacacgaag aactgcacga tctacggcga aggaaggatg gcgctcggcg ccgatctcgg
     7801 aaaagtattg ctcacgccaa tcgggaatgc cacgcacaat acggtcgatc attccacgtc
     7861 attcgacctc gtaatgggaa ttgacttttt catccccgaa aagctgctga attacgtgct
     7921 cgacgacatt gacgcctcca cgtcgctcgc cggtacagac ccgacgcgca ccacgtttca
     7981 gcacgcgctg acggaaatgg tcggaaaaga acgcgccgac aagcttattt ccaatcttac
     8041 ggtcttcaat tcctacaaga aattccccga tgagctgaag tacacgttct tcttcactga
     8101 cgtgcagatg aactggaacc agaaaacgaa ggcgtacatc tccgacggca agcttgggct
     8161 gggtaacagc gataaaaagg aaatcaataa atttgtgccc ggaaccatta tgctggaaag
     8221 agtgaggagc ggtgacaggc tcacgatcta tttcgaactg gataatggta aatggtatac
     8281 gttctattac gcaaacggaa tgatgagcgc ttattcgtcg aacacgaagt tcatggacga
     8341 gctgaaagcc atgaaggatg atgacaaggt ggcaccggat gattacgaca aagaccgctt
     8401 gtcggaaaaa ggacagaaag ccaaatacaa aataacagcg ggaggtccgc aggatctgtc
     8461 gaggttaaag aacaagctga aaaaagccag tgacggtgga gatgacagcg ggggaggaga
     8521 cggcgacgat tcaggaaata aataaatgac gacaaccagt attatcgcac tgctgatcgc
     8581 ctacctgctc gggtccattc cttccgcggt gtggataggg aagttgtttt tcgggaccga
     8641 tgtgcgggag cacggaagcg ggaatgcagg tgccacgaac acgttccgtg tgctcggcaa
     8701 aaaagcgggg atacctgttc ttctccttga tattctgaaa ggatttctcg ccgtgaacct
     8761 cgcgtggctc gtcaccgaag cgcccccgca caccagtgat ttcgtggatc tgcagatcgt
     8821 gctgggtgtg gccgccgtgc tcgggcatat tttcccggtg ttcgcaggtt tccgcggggg
     8881 aaaaggcatc gcaacgttgt tcggcgtaac gctggcggtg catccgcaaa cctgcctgct
     8941 cgcgaccggt gttttcctgc tggtcttcct ttccacgaat tacgtttcac tcggttccat
     9001 cagcgccggg ctttccatgc cgctgttcat gatgttcttt tttgccagtg aagcgttccc
     9061 ttcgatgatc gcctattgcg taatggtttc catgcttatc ctcatcacgc accagaaaaa
     9121 catagaacgg ctgctgcgta aggaagaatc gaaaatgcgg ttgacgagaa ggaaaaacag
     9181 cggggaagac gcatccgatg ccggggtgaa atagcgggtt tttcaacttc cttttcccgt
     9241 ttttttagtg tctcgaattg cttttccggc gttatgtaca gcagtggccg ggagcggttt
     9301 tgtatttaat taacgttttt gtgtggaaat ccttacaaaa cggcatctga gcattacagc
     9361 ctttatttcg taaattaggg cataactatt gtgggacagg tcgttataat aacggctatc
     9421 atagcgttgg ggcgctaacc tctgatatgc gcagaaggtt tcttattgtt tttttaatga
     9481 ctgctttagc ggtcctgcta cgccctttcc ccgttttttc ccaggctccc ggttctgaag
     9541 aagatgtgat caggaatgcg gacgccgcgt tcgataaagc agattatgcg aaagcgatgc
     9601 cgctttattc gcagctgctc gccaatcacc ccgctgatcc gaactacaat tacaagtacg
     9661 gcgtttgcgt cctctacgcc agctccgaaa aagacaaagc gcttcccttc cttgaaaaag
     9721 cttccgccga tccgaaagca gatgccgacg tctggttcta tctcggcaaa gcgtatcacc
     9781 tgaattacca gttcgaccag gccattgtcg cttacacgaa atacaagcag ctggtgggtg
     9841 aaaagaaggc tgagaaaatg caggtcgaca accagatcgc gatgtgcaag accgggcgga
     9901 aattgctgaa ggcgatcacc gatatcaccg tgctggaaaa agcagagctc aacgagcagg
     9961 atttttacaa gcactatgac ctgaagagcg cgggttacag cgggcagctg ctcgccgagc
    10021 cggatgaatt caaaacagcg ctcgacaaga aaaaaggagt tacatcgatc atctacctct
    10081 cgggtgaaaa gagcgagctc tatttttctt cctacggcga tgatgaatcg cagggaaaag
    10141 acatttacgt ggtcaggcgc ctgccgaacc agaaatgggg gaagccgcag aatgtcggct
    10201 acccgatcaa tacggaatac gacgaggatt acccgttcct catgcccgac gggaaagtgc
    10261 tctatttctc ctcgaaggga cacaattcca tgggcggcta tgatgttttc cgcgcggagc
    10321 tgaacgaaga aaccaatacg tgggaaaaac ccgtgaacct ggattttgcg atcaattcgc
    10381 cggacgacga catcatgttc gtcagcgatg cgaacgagca gacggcgtgg tttgcctcga
    10441 acaggaacag tcctgaagga aagatgacgg tttatcatgt cgtgatccag cgcaagcccg
    10501 ttcagaactg catgatcagc ggaacattcg tttccaccga cggagatccg aatgaaaaag
    10561 cgaagatcac cgtcaagaac gaggatacga atgaacccat cggcgtgtac acgagcaatc
    10621 aatccagcgg ctcgtatttc atgaaccttc ccaacaacgg cggaaaatat tcattcactg
    10681 tcgagcattc cggaattcat acgcagacgc agaccgtgat cattccgccg cagtacgaga
    10741 tcaagaccgt caaccagcgc atacattacg tcgatgtgaa cggtgatcag cagctcgtca
    10801 tcgaaacgga tttttccagc gacaccagca cgctcgatcc gcttttcctg aaagacaaag
    10861 cgaagctcga tgtgaattca ggacccgacg cctaccaggt ggtcgatatg ggaaacccgg
    10921 acaacgcagg aaaaaatccg ggcaatacca cggaccccga caacagcaac gttaccgatc
    10981 cgggagatga tccttcggat tcgatcgatc cggctgacag cgttccttct tccaacgcgg
    11041 gaaaagtcac cgacgcagag ctggtgaaat ctgcgtacga cgatgcgaat gcgacgaagg
    11101 cggaggcgga ggatatgcag aaccaggcga acaatgcgta cgcctatgca gccgatctcg
    11161 accggcaggc gaaagccatg caggattccg caaccaatgc taaaaaagct gcggacgcaa
    11221 tggcggatgg cccggataaa gtggctgcca cgaagcgcgc ggacgacctg caggcgcagg
    11281 cgaattcatt gcagtcgcag acagtggccg ctttcaacgt ggcgcaggcg atggataccg
    11341 acgcgcagaa caaaaagaac gaagcggata aaacgcagca atatgcagca agtcttgaca
    11401 aagcggtgaa gtcaaatgat ccgaaagcgc tcgaccaggc gcagaagcag gcggatgacc
    11461 tgcagcaata ttcggtaacg accacgcaga aagatcagac ggtgcagaac atttccaaac
    11521 tggatgatca gaaacatgcc gatctgaaaa aagcgcagga cgatctcgat ctcatgcagg
    11581 cggacaacaa ggataattac gatcgcattg cgcagcttca gaaagacaag gacaaggaaa
    11641 aagacaaaga cctgagagcg ggctacgacg accagataca gggactgaag gatgatatcg
    11701 acgacaacaa cgcgaagatc gcgaagcagc aggcgtacgt caattcgctg cagaacgatg
    11761 tgaatgacct cgacaaccag ttgcacggcg tcacccaggc ggctgcacag cccaagacca
    11821 atacgggcgc tgttgtttcg ccgcagcaga aaaaggacct
//
LOCUS       DASTSF010000539         4730 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1219860,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000539 DASTSF010000000
VERSION     DASTSF010000539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4730
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1219860"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..554
                     /locus_tag="VFU15_15375"
     CDS             <1..554
                     /locus_tag="VFU15_15375"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEU4719224.1"
                     /translation="HSMYISSLCWMMQDFNNAYLDPIIDSLANRIRTDVYADSLKFYT
                     NQQFEDDMTMDVQVPGGPGGGWIPGLKNFIAARNASMTSQLAPFGCYLSVNEIVPSPS
                     LSLFPNPGNDLVTVTLPSGWTTSNSTLHVFDFSGREILNDDRRDGKTSFGMDVSSFAD
                     GLYLVEIRREDGTSLSSRMIVSH"
     gene            675..1064
                     /locus_tag="VFU15_15380"
     CDS             675..1064
                     /locus_tag="VFU15_15380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719225.1"
                     /translation="MTRKIALAILTIGFFSFSCNNGPGPGGKASITGKVWAQRYVNNC
                     TLLAGEYWDPDEDVYIIYGDDPSYGDRIKTGPDGTFQFNYLREGKYTVYAYSNVCSVA
                     QREAVKMTVEITGKKQNVQLDTLKILK"
     gene            1066..1671
                     /locus_tag="VFU15_15385"
     CDS             1066..1671
                     /locus_tag="VFU15_15385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719226.1"
                     /translation="MHPRLLLFLAFVFPATVSFAQSKKQIKDLKIKSVTETVTLYKDG
                     KETSTYKASYKTFDKDGNVLSDITYNPDGSVRRQETNTYSGKNKTVEIVEKPNPGPSS
                     DEDDTDDSPSRYKKTAWKYNANGDKTEEVVYDASGNVVKKTTYAYSTSGDKMIEVTYD
                     GTGKMLKKIDYGYDSRGLRTEKKIYATGDVLVKSVKYTYTY"
     gene            1671..2438
                     /locus_tag="VFU15_15390"
     CDS             1671..2438
                     /locus_tag="VFU15_15390"
                     /inference="COORDINATES: protein motif:HMM:NF020916.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HEU4719227.1"
                     /translation="MNSAALRNILNQFDPISLEEMDRVLLMDRTDLKFNFHDSRLPEL
                     LDRVKDAYLALDVADTRMTRYETLYYDTEDFSFYSEHHNEHANRYKIRLRRYVESDTN
                     FFEIKFKNNKGRTIKTRVLRKDRHHDIEENSEKLLRDITPIEAGSLRPKLWVNYNRIT
                     LVNKTEEERVTVDFNMEMRMNENTVDFPSLVIVEVKQVRPRETALVRALRKMHIHEGG
                     TSKYCIAVSKLVSDIKKNNFKPDLITIKKIINDNPAA"
     gene            2419..3210
                     /locus_tag="VFU15_15395"
     CDS             2419..3210
                     /locus_tag="VFU15_15395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015027755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HEU4719228.1"
                     /translation="MTIRLLKFLLPLILLLFSPAPATLHAQTGGDNASVSSSSDQSTD
                     DNSDDKKKKRKDKDEETDFFTFSIDKFNPAFFARLGVDIVCMLVLILFIYLPVYKRRD
                     HVFTFFMFNVTIFIITFTLNTKSGFSTGAAFGMFAVFSLLRYRTEDISARDMTYLFIV
                     IALGLISSITKGDLLETIVINGIIVLGAFMLDGNLFFKTEFVKTIQYENIDLIKPENY
                     DQLLADLTARTGLKVHKASIGHVDFLRDTATIKIYYYENTNQAPE"
     gene            3222..3905
                     /locus_tag="VFU15_15400"
     CDS             3222..3905
                     /locus_tag="VFU15_15400"
                     /inference="COORDINATES: protein motif:HMM:NF022142.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2490 domain-containing protein"
                     /protein_id="HEU4719229.1"
                     /translation="MKRPGLFLFLSFLLCRPLFAQHQDLGSWMTFSANKGLFGKLEFN
                     FDQEFRLRDNLTNVNLFYTNLGFSYKVNKYMKVSATYRFIDKHKSDGSYGLRNRFFTD
                     LTFKIKPGKFNLSYRFRCQEEWRGAGYNDTYGNVPEIYIRNLFKVAYKLTGHWSPYAG
                     TELRWQLQNPRIPYYNGFDRSRFFAGTDYKINDHNTAGVYFLYQLEWNVVDPETLYII
                     GLEYSIDID"
     gene            complement(4004..4489)
                     /gene="ispF"
                     /locus_tag="VFU15_15405"
     CDS             complement(4004..4489)
                     /gene="ispF"
                     /locus_tag="VFU15_15405"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005833434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HEU4719230.1"
                     /translation="MSAPFRIGFGFDVHPLVAGRPLWLGGIRIESEKGCDGHSDADVL
                     LHAICDALLGAAGLRDIGFHFPNTDAAFKNIDSKILLEKTIALLKEKGWSVGNVDATL
                     VMERPKISPHIPAMVKTIASIMKVEENCVSIKATTNEKLGYVGSENGANAYAVALVTK
                     I"
     gene            complement(4549..>4730)
                     /locus_tag="VFU15_15410"
     CDS             complement(4549..>4730)
                     /locus_tag="VFU15_15410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014791544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719231.1"
                     /translation="YKGNRQFLTLGAGIKYTIFGLDFAYLIPTNGQRSPLQNTLRFTM
                     TFDFDGVKSDSADPK"
BASE COUNT         1222 a         1323 c         1092 g         1093 t
ORIGIN      
        1 ttcacagtat gtacatctct tcgctgtgct ggatgatgca ggatttcaat aacgcttatc
       61 tcgatcccat catcgacagc ctggccaacc gcatccgcac ggatgtttac gccgactcgc
      121 tgaaattcta taccaaccag cagttcgagg acgacatgac catggatgtc caggtacccg
      181 gcggacccgg cggcggatgg atacccggcc tgaaaaattt cattgcggca cgcaacgcca
      241 gtatgacgtc gcagctcgcc ccgttcggct gctatctttc ggtgaacgaa atcgttccat
      301 ctccttcgct ttcccttttc ccgaatcccg gcaatgattt ggttactgtc acgcttccct
      361 ccggctggac gacttccaat tccacgctgc atgttttcga tttttccggg cgggaaattc
      421 taaacgatga caggcgcgac ggaaagactt cgttcgggat ggatgtttca tcatttgccg
      481 acggactcta cctcgtggaa atacggcgtg aagacggaac ctctctttca tctcggatga
      541 tcgtcagcca ctgaccggcc cgttttgccg ttagctaaaa acgaatatct ttacaatagc
      601 ttaatattgc tgccttccgg cggtgacctg cggtgtaggc tatcaatatc tttgtttcac
      661 acagctgttt ttttatgacc cgaaaaattg cactggcaat actcacgatc ggattcttct
      721 ctttttcgtg caacaatggt cccggccctg gcggaaaagc ttccatcacg gggaaagtct
      781 gggcgcaacg gtatgtcaac aactgcacac tccttgccgg ggaatactgg gatccggatg
      841 aagatgttta tatcatttac ggcgacgatc cttcctatgg agaccgcata aagacaggtc
      901 ccgacggaac cttccagttc aactacctgc gcgaaggcaa gtatacggtc tacgcgtatt
      961 cgaacgtgtg ctccgtggcg cagcgcgagg ccgtaaaaat gaccgtcgag atcaccggca
     1021 aaaagcaaaa tgtacagctc gatactttaa aaatcctcaa gtaacatgca tccccgactg
     1081 cttcttttcc tcgccttcgt cttcccggcg acagtttcct ttgcccaatc caaaaagcag
     1141 atcaaggatc tgaaaataaa aagcgttacc gaaacagtta cgctctataa ggacggcaag
     1201 gaaacttcga cctataaagc atcttacaaa acattcgaca aggacgggaa cgtgttgagc
     1261 gatattacgt ataacccgga cggcagtgtg agaagacagg agaccaacac ctacagcggg
     1321 aaaaacaaaa cggtcgagat tgtcgaaaaa ccgaacccgg gtccgtcttc cgacgaggat
     1381 gacactgatg attcgccttc caggtacaaa aaaacagcct ggaaatacaa cgccaacggg
     1441 gataaaaccg aagaagtcgt ttacgacgct tccgggaatg tggtgaaaaa gaccacgtat
     1501 gcctatagca ccagcggcga taaaatgatc gaagtgactt acgacgggac aggcaaaatg
     1561 ctgaagaaga tcgactacgg atacgattcc cgcggtctcc gtaccgaaaa gaaaatatac
     1621 gccacgggcg acgtgctcgt gaaatccgtg aagtatacgt atacctattg atgaattccg
     1681 cggccctgcg aaatatcctg aaccagttcg atcccatttc cctcgaggag atggaccgcg
     1741 tgctgctcat ggaccggacc gacctgaagt tcaactttca tgattcgcgc ctgccggagc
     1801 tcctcgacag ggtgaaggac gcttatcttg cgctcgacgt agccgatacg cgcatgaccc
     1861 gctacgaaac gctttattac gataccgaag atttctcctt ctacagcgaa caccacaacg
     1921 agcacgccaa ccgttacaag atccgcctgc gccgctacgt ggaatccgac acgaatttct
     1981 tcgaaatcaa attcaagaac aataaaggcc ggacgataaa aacacgtgtg ctgcgcaaag
     2041 accgccacca cgacatcgaa gagaattcgg aaaagctgtt gcgcgacatc acgcccatcg
     2101 aagccggatc gcttcgtccg aagctctggg tgaattacaa ccgcatcacg ctggtgaata
     2161 aaacggagga agaacgcgtt acggtcgact tcaacatgga aatgcggatg aatgagaaca
     2221 ctgtcgattt tccttcgctg gtgatcgttg aagtgaagca ggtgcggcca agggaaaccg
     2281 cgcttgtgcg cgcgctgaga aaaatgcata ttcacgaggg cggaacgagc aaatactgca
     2341 tcgcggtcag caagctcgtg tccgacatca aaaagaataa tttcaaacct gacctcatca
     2401 cgatcaaaaa aattatcaat gacaatccgg ctgcttaagt tcctccttcc gcttatcctg
     2461 cttcttttct cacccgctcc cgcaacgctg catgcgcaaa cgggcggcga caatgcatcg
     2521 gtttcttcgt catccgacca gtcgacggat gacaacagcg atgacaaaaa gaaaaagaga
     2581 aaagacaagg acgaggaaac ggatttcttc acgttcagca tcgacaagtt caatccagct
     2641 ttttttgccc gcctcggcgt ggatatcgtg tgcatgctcg tgctgatcct gttcatctac
     2701 ctgcccgttt acaagcggcg cgatcatgtt ttcacattct tcatgttcaa cgtgacgatc
     2761 ttcatcatca cgttcacgct gaacaccaaa tcgggatttt ccaccggcgc cgcgttcggc
     2821 atgttcgccg tattctcgct gctgcgatac cggacggaag atatttccgc acgcgacatg
     2881 acctacctct tcatcgtcat cgcgctcggc ctcatcagct ccatcaccaa aggcgacctg
     2941 ctcgagacga tcgtcatcaa cggcatcatc gtgctgggcg cattcatgct cgacggaaat
     3001 ctctttttca aaaccgagtt cgtaaaaacc atccagtacg agaacatcga cctgatcaaa
     3061 ccggagaatt acgatcagct gctcgccgac ctcacagcgc gtacgggact gaaagtgcac
     3121 aaggccagca taggccatgt tgattttctc cgcgacaccg cgacgataaa aatatattac
     3181 tatgagaaca cgaaccaggc gcccgaataa tcaaccgcat catgaaacgc cccggcctct
     3241 tcctctttct ttccttcctg ctttgccgcc cactgttcgc gcagcaccag gatctcggct
     3301 cgtggatgac gttcagcgcc aataaaggtc ttttcggaaa gctcgaattc aatttcgacc
     3361 aggaattccg ccttcgcgat aatcttacca acgtaaatct cttctacaca aatcttggct
     3421 tcagctacaa ggtcaataaa tacatgaaag tatccgccac ctatcgcttc attgacaagc
     3481 acaagtcgga cggaagctat ggtttgcgca accggttctt caccgatctc acgtttaaga
     3541 tcaagcccgg gaaattcaac ctgtcgtacc gcttccgttg ccaggaagaa tggcgcggcg
     3601 ccggctacaa cgatacatac gggaatgttc cggagatcta catccgcaac cttttcaaag
     3661 ttgcctacaa gctcaccggt cactggtccc cgtatgccgg cactgaatta cgctggcagc
     3721 tgcagaaccc gcgcattcct tattacaacg ggttcgaccg ctcccgtttt ttcgccggaa
     3781 cagactacaa gatcaacgac cacaacacgg caggtgttta ttttctttac cagctcgaat
     3841 ggaacgtcgt cgatcctgaa acactttaca tcatcgggct ggaatattcg atcgatatcg
     3901 actgagtttc gcagggaaag gaacaatttt catcttcacc tgccccagca tcgcaacccg
     3961 caggtttgct tttcatacac aacctggtct cgcgttccaa tacttagatc ttcgtcacca
     4021 gcgccaccgc atacgcattc gccccgttct ccgagccgac atatcccagc ttttcattcg
     4081 tcgtcgcttt gatggaaaca caattctctt ccaccttcat gatcgaagcg atcgttttca
     4141 ccatggcggg aatatgcgga ctgatcttcg ggcgctccat caccagcgtg gcatctacgt
     4201 ttcccactga ccagcccttc tctttcagca gcgcgatcgt tttttcaagc aatatcttgc
     4261 tgtcgatatt tttgaacgcg gcgtccgtat tcggaaaatg gaaaccgata tcgcgcaacc
     4321 ctgcagcacc aagcagcgcg tcgcagatcg cgtgaagcag cacgtcagca tcggagtggc
     4381 cgtcgcatcc tttttccgat tcaatgcgga tcccgccgag ccacagtggt cttcccgcaa
     4441 caagcgggtg aacatcgaaa ccaaaaccga tcctgaaagg tgcgctcata cttgaaataa
     4501 aaaaacgctg ttaccggtaa gcaacagcgt ctcgtggttc atgctttttt atttagggtc
     4561 cgcgctatcg gatttcacac cgtcgaagtc gaacgtcatg gtgaaacgca acgtattctg
     4621 aagcgggctg cgctgtccgt tcgtcgggat gagataagcg aaatcaagac cgaagatcgt
     4681 gtacttgatg ccggcgccga gtgtcaggaa ctggcggttg cctttgtatt
//
LOCUS       DASTSF010000540         3648 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1233046,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000540 DASTSF010000000
VERSION     DASTSF010000540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3648
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1233046"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..533)
                     /locus_tag="VFU15_15415"
     CDS             complement(<1..533)
                     /locus_tag="VFU15_15415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010255704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU4719232.1"
                     /translation="MATEIKNVEYGLEKIFEGAQDFLPLLGTDYVEFYVGNAKQSAHF
                     YKTAFGFQSCAYAGLETGLKDRTSYVLSQGKIRLVLTTPLVSDSPIAEHIRKHGDGVK
                     VIALWVEDARKAFEETTKRGAKPFMEPVVEKDNFGEVVRSGIYTYGETVHMFVERKKY
                     DGPFLPGFQKWESTYNPA"
     gene            complement(677..1834)
                     /locus_tag="VFU15_15420"
     CDS             complement(677..1834)
                     /locus_tag="VFU15_15420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HEU4719233.1"
                     /translation="MPIYHKLGKIPSKRHTVFKNPDGRFYYEQLFGTEGFNGFSSLLY
                     QVHRPTQIKEIVSSKNVAPEIAIEKNIKPLLLQGFKTETKDDFLDSRTPMMVNSDVLI
                     GLAAPRKSMRSYFYKNADADEMLFIHKGKGKLRTFMGNIDFEYGDYLIIPRGMIYQID
                     FESDDNRIFYVESHHPMYTPKRYKNSSGQLLEHSPFCERDFKLPSELETYDEKGDFHI
                     KVKKENMLHDIVYATHPFDVVGWDGYNFPWGFSIHNFEPITGRVHLPPPIHQTFETSA
                     FVVCSFCPRLYDYHPLAIPAPYNHSNIDSDEVLYYVDGDFMSRNNIKQGHITLHPKGI
                     PHGPAPGAMERSIGQKETQELAVMVDTFRPLMLTKQAMGIDDGVYYKSWVE"
     gene            complement(1908..2945)
                     /locus_tag="VFU15_15425"
     CDS             complement(1908..2945)
                     /locus_tag="VFU15_15425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020771177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HEU4719234.1"
                     /translation="MSALYNKVSRAEMRRRLMAEDFQRITLSFYRYVIIGDPVEMRNT
                     LWREWTELNVFGRVYIAREGINAQISVPEQYFETFRRTLYAHPEFENVPFKIAVEDNG
                     KSFFLLAIKVKKKIVADGLKDDSFDVTNVGKHLTAVEFNEAMETPGVVVVDMRNHYES
                     EVGHFENAILPQADTFKDELPMVLDELKGKENNKILMYCTGGIRCEKASAFLRHHGFR
                     DVNQLYGGIIEYARQVKQTGLPSKFRGKNFVFDERLGERITDEVIAHCHQCGASADTH
                     VNCANPDCHVLFIQCSACGRKFEGYCSGECRNWAKLPEAEREKLKKEGKIVNARALYH
                     SRLRPAFGSKW"
     gene            3066..>3648
                     /locus_tag="VFU15_15430"
     CDS             3066..>3648
                     /locus_tag="VFU15_15430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006989347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEU4719235.1"
                     /translation="MPSETILVIGSSGQIGTELVMELRARYGNDNVIASDLKNAAPQV
                     MESGPFETLDALDKQKLGELVKKHNVKQVYLLAALLSATAEQNPMFAWKLNMESLFNV
                     LELGREKRLSKIYWPSSIAVFGPTTPRINTPQYTVIEPATVYGISKQAGERWCEWYFH
                     KHGVDTRSLRYPGLIGWKSAPGGGTTDYAVHIFH"
BASE COUNT          817 a          989 c          933 g          909 t
ORIGIN      
        1 gccgggttat aggttgattc ccatttctgg aaaccgggaa ggaacgggcc gtcgtatttt
       61 tttcgctcga cgaacatgtg aacggtttca ccgtacgtgt agatcccgct gcgcaccact
      121 tcgccgaaat tgtctttttc caccaccggc tccatgaaag gctttgcgcc gcgtttcgtg
      181 gtttcttcga atgcttttcg cgcatcttcc acccacagcg cgatcacttt cacgccgtcg
      241 ccgtgcttcc tgatgtgctc cgcgatcggc gaatcgctca ccagcggcgt tgtcagcaca
      301 agcctgatct ttccctggga aagcacatac gatgtgcggt ctttaagtcc cgtttcgagc
      361 ccggcgtaag cacacgactg gaagccgaat gcggttttat aaaagtgcgc cgactgtttg
      421 gcattgccga cgtaaaattc gacgtaatcg gtcccgagca gcggcagaaa atcctgggcg
      481 ccttcgaaaa ttttttcgag cccgtattcc acgtttttga tttcagttgc cattgtaact
      541 gttttttgaa tgttttttga aatgatcgtt cacgggtaaa ttgaaatcta tctctctgac
      601 cacatggccc gcgtatgggc cgaaataagc gactgatgca tggagaaatt catgtattaa
      661 agttacgcaa tagaatttat tccacccatg acttgtaata aacgccgtcg tcgatgccca
      721 tcgcctgctt cgtcagcatc agcggacgga atgtatccac cattacggca agctcctgtg
      781 tttctttctg cccgatactg cgttccatcg cgcccggcgc gggtccgtgc ggaattcctt
      841 tcgggtgcag cgtgatgtgt ccctgcttga tattgttccg gctcatgaaa tccccgtcga
      901 cgtaatacag cacttcatcc gagtcgatgt tgctgtggtt gtaaggcgcg ggaatggcaa
      961 gcgggtgata atcatacaac cgcggacaga acgagcacac aacaaaagcc gatgtttcaa
     1021 atgtctggtg aatgggcggc ggcagatgca cgcgcccggt gatcggttcg aaattgtgaa
     1081 tggaaaatcc ccacgggaaa ttataaccgt cccagcccac cacgtcgaac gggtgcgttg
     1141 cgtacacgat gtcgtgcagc atattttcct ttttcacctt gatgtggaaa tcgcctttct
     1201 cgtcatacgt ttcgagctcc gaaggcaatt tgaaatcacg ctcgcagaac ggggaatgct
     1261 cgagcagttg cccggaagaa ttcttgtacc gcttcggtgt gtacatcggg tgatgacttt
     1321 caacatagaa aatacggttg tcgtccgatt caaaatcgat ctggtagatc atcccccgcg
     1381 gaatgatgag gtaatcgccg tattcgaaat cgatattccc catgaacgta cgcagcttgc
     1441 ccttgccttt gtggatgaaa agcatttcgt ccgcatcggc gttcttgtag aaataactgc
     1501 gcatgctttt ccgcggagcg gccaggccga tcagcacatc gctgttcacc atcatcggcg
     1561 tgcggctgtc gaggaaatca tctttcgttt cggttttgaa tccctgcagc aggagcggct
     1621 ttatgttttt ctcgatggcg atttccggcg cgacgttttt cgacgacacg atctccttga
     1681 tctgcgtggg cctgtgcacc tggtaaagca gcgaggaaaa tccgttgaac ccttcggttc
     1741 cgaaaagctg ttcgtaataa aaacgtccgt caggattttt gaaaaccgta tggcgtttcg
     1801 aagggatttt tccgagttta tgatagatag gcatagcaat gaatttggaa atccaaagct
     1861 atctcccgca acacgattct tcaatgacaa ttatcaggga agcagtctca ccactttgaa
     1921 ccgaacgcag gacgaaggcg gctgtggtaa agcgcgcgcg cattcacgat cttcccctct
     1981 ttcttcagct tttcgcgttc cgcttccggc agcttcgccc agttgcggca ttccccggaa
     2041 caatagcctt cgaatttccg gccgcaggcg gagcattgaa tgaacagcac atgacaatcg
     2101 gggttggcgc aattcacgtg cgtatccgcg gacgccccgc actggtggca atgtgcgatc
     2161 acttcatcgg tgatgcgctc gcccaggcgt tcgtcgaaca caaagttttt cccgcggaat
     2221 ttcgacggaa gtcccgtctg cttcacctgc cgcgcatatt cgatgatgcc gccgtaaagc
     2281 tggttcacat cccggaatcc gtggtggcgt aaaaacgcgc tcgctttttc gcagcggata
     2341 ccgccggtgc aatacatgag aattttattg ttctcttttc ctttcagctc atccagcacc
     2401 atcggcagct cgtccttgaa cgtatccgcc tgcggcagga tcgcgttttc aaaatgcccc
     2461 acttcgcttt cgtagtggtt gcgcatatcg acgacgacaa cgcccggtgt ttccatcgcc
     2521 tcgttgaatt caactgcggt aaggtgcttc ccgacgttgg tcacgtcgaa ggaatcgtcc
     2581 ttcagcccgt cggctacgat ctttttcttc accttgatcg ccagcaggaa aaatgatttc
     2641 ccgttatcct ccaccgcgat cttgaacgga acgttttcaa attccgggtg cgcatacagg
     2701 gtgcggcgga acgtttcgaa atactgctcc gggacgctga tctgcgcatt gatgccttcg
     2761 cgtgcaatgt atacgcggcc gaaaacattc agttccgtcc attcgcgcca taacgtgttc
     2821 cgcatttcca cgggatcgcc gatgatgacg taacggtaga acgaaagtgt gatccgctga
     2881 aaatcctctg ccatgagacg gcgtctcatt tcagcccggc tgaccttgtt gtacaatgcg
     2941 ctcatttcgg actgcaaagt taaatgctcc gcaaattcac gtgatgacaa aaatcaggaa
     3001 gcaaattacg gccgtaattt ttcatccgga atgttacctt tggaacatct tcattcaacc
     3061 aaaaaatgcc ttctgaaacc atactggtga tcggctcttc cgggcagatc gggacggagc
     3121 tcgtcatgga acttcgtgca cgctacggga acgacaacgt gatcgcctcc gatctgaaaa
     3181 atgccgctcc gcaggtgatg gaaagcggtc cgttcgaaac gctggatgcg ctcgataagc
     3241 agaagctggg cgagctggtg aagaagcata atgtgaagca ggtgtacctg ctcgccgcgc
     3301 tgctttcggc cacggccgag cagaatccca tgttcgcctg gaagctgaac atggaaagtc
     3361 ttttcaatgt gctcgaactc gggcgcgaaa agcggctgtc gaagatctac tggcccagct
     3421 ccatcgcggt tttcggtccg acgacgccgc gcataaacac accgcaatac acggtcatcg
     3481 aacccgcgac ggtttacggc atcagcaagc aggccggcga acgctggtgc gaatggtact
     3541 tccacaagca cggcgtggac acgcgaagcc tgcgttaccc gggactaatc ggctggaaat
     3601 cagcaccggg aggaggaacc acggattatg cggtccacat cttccacc
//
LOCUS       DASTSF010000541        12625 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_450301,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000541 DASTSF010000000
VERSION     DASTSF010000541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 12625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12625
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_450301"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            56..1006
                     /locus_tag="VFU15_15435"
     CDS             56..1006
                     /locus_tag="VFU15_15435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719236.1"
                     /translation="MATFAHIINPVRAPAGSELDIVQPATFATVKRALGEAEGKVQVD
                     FLSAQFPEDHPVIPSWVKKTPDLERSLADLGSFAKPRRLPVLRDILDRAMQASDADYI
                     IFSNADICLMPSFYLVLNEYAEKGYDAFAINRRRIPARFAGETRMELLYAEAGEKHPG
                     YDTLVFRRSLYEKFVLGNVCTGIPRFDTILIHNFYAFAENFRLFTGKHLTFHIGMTLC
                     KRWGDNDQYAFNEKEYAMVRRKLYPHFRIENFPGANLPFLRRHFKWFLNPTFSYPMMF
                     RLDLSQLSRPRRKREKKEICGLQNRWCEFGSRFINFDDEY"
     gene            complement(1010..1831)
                     /locus_tag="VFU15_15440"
     CDS             complement(1010..1831)
                     /locus_tag="VFU15_15440"
                     /inference="COORDINATES: protein motif:HMM:NF018669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase family 2 domain-containing
                     protein"
                     /protein_id="HEU4719237.1"
                     /translation="MSAAPDTEKTLLFLHIQKTAGTTLLNIITRQYEKRRTLNLYDPR
                     TAEKITASLSEEERKNIRLVNGHFSFGLHRYFPGKCDYITILRDPLERTVSQFHFILD
                     FKTHPLHEEYNRNGYTLLHVIRNRMMIHLDNYMVRVISGKDDVPFGECTEEMLDTALE
                     NLEKYFTLTGLQENFDAFVLSLADLYHWKKFLYYRRQRTGVSRPRVQDLDAETLEAVR
                     SCNVLDQKLYDIIAPKVREELRKKGPAFEARVEKFKRKNQLMNRYLGWWPRALTP"
     gene            complement(1889..2716)
                     /locus_tag="VFU15_15445"
     CDS             complement(1889..2716)
                     /locus_tag="VFU15_15445"
                     /inference="COORDINATES: protein motif:HMM:NF018669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase family 2 domain-containing
                     protein"
                     /protein_id="HEU4719238.1"
                     /translation="MNTEPNLPVAPHLLFLHIQKTAGVTMHNIIGRQYPKKNTLSIYH
                     TEKGLAELKRLTPERASQIRLLKGHFAFGAHRFFREPCEYITMLRDPVERTISHFHFV
                     IDSVTHNYHDMIVDSGYSLLEVLENGAIPRLDNKMVRMLSGNDSAPYGACTETMLDDA
                     MTHLEKNFSVVGRVKNFDEFVLMCSERFHWNGYIFYRKRNAGRSRPKTEEIDKKTMDA
                     LRAHNTLDQKLYDTWSPIIEDRIARQGARFNARLASFKKKNQLLNRVLGWWPDFLTP"
     gene            complement(2706..3443)
                     /locus_tag="VFU15_15450"
     CDS             complement(2706..3443)
                     /locus_tag="VFU15_15450"
                     /inference="COORDINATES: protein motif:HMM:NF016974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl/asparaginyl beta-hydroxylase
                     domain-containing protein"
                     /protein_id="HEU4719239.1"
                     /translation="MSRSTHPWYSFRGGSYPGNAPSFYDGSAFAWVAETESRFPGLME
                     AAKGFIGSFEERSAPYFNANLVSKKGSWRQGTFYFWGLKNEELCRAVPAIDDFIWSVP
                     GMLSGGLSLLKAETDILPHFGDTDAVMRCHLGLVIPAPLPECGLEVNGEQRSWEKGKW
                     LLFCDAHYHRAWNHTKQTRIVLIVDVLRPGYESRKREICSNVRSMLSLQRLEQAFPLF
                     RKFPGKIRGLVRRFFFRIHYFRFKNEY"
     gene            complement(3440..4096)
                     /locus_tag="VFU15_15455"
     CDS             complement(3440..4096)
                     /locus_tag="VFU15_15455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719240.1"
                     /translation="MILTGYNVRLERLTHEKIELVRQMRNMEKIVRHMEYREYITPEM
                     QEKWFRSIDNTENHYYIISSGEKEVGLINGCKVNWKKKETGSGGIFIWDDEVLRTEIP
                     LAASLLLTDTSFYLGLERTYVKILRDNAAAISYNHNLGYELLPGQENSVNQEYVLTQE
                     NYEAKTKKIKEIFHRKWGDRMTIIFDNPEHPVTRFLEEKIRALPEQVLAHLELITPGR
                     "
     gene            complement(4141..5061)
                     /locus_tag="VFU15_15460"
     CDS             complement(4141..5061)
                     /locus_tag="VFU15_15460"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU4719241.1"
                     /translation="MRIQFLSIVIPVYNSAQMLEQLYEEISSAVSGVVPFEVIFVNDR
                     SRDESFAVIQKIRDANPDHVTGISLLKNSGQHNAISCGLGFAKGDAIITMDDDLQHPP
                     SEIPKLIAEYERSRCDVVYGDYPNKKHPGWRKTGSWLLRRGSKTMGEGPGRGSSFRIL
                     SKSIAEKIIQHNRTGFIFIDEIIGWYSGNVSYINVEHHPRRSGKSTYSGGKLISFYFD
                     IIINYSAFPLKAMTWVGLISSCLTFMLGLRFIYKKLVHGSAPGFTAIIVTVLFSTSVL
                     MLCLGIIGQYMYKLYQTRNRRPPFTVDKVV"
     gene            complement(5063..5812)
                     /locus_tag="VFU15_15465"
     CDS             complement(5063..5812)
                     /locus_tag="VFU15_15465"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU4719242.1"
                     /translation="MKTPFHLDDKIILVTGASSGIGRQVAISISRMGGTCIISGRNEE
                     ALSETLGSLEGKGHISIAADLLDETGRKRLLEQLPATDGLVHCAGNVKAFPIRFLDQV
                     RIDEMMKLNFDAAVLLTAGLLKNKKLKRDASLVYLSSISSKYPPKGGSMYGSAKAALE
                     TFVKTLAQEIVSSGMRANSISPGMVKTPLYDRAEAAVSKAEMDEHMKGYPLGAGLPED
                     IANAAIYLLSPASRWVTGINIILDGGFLSGG"
     gene            complement(5809..6861)
                     /locus_tag="VFU15_15470"
     CDS             complement(5809..6861)
                     /locus_tag="VFU15_15470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HEU4719243.1"
                     /translation="MAIFSVRGTQVKGIAACVPSAKEYNRDYDWISEAERNLLIKTTG
                     ISSRHIAGKGTTTTDLAVPAAELLIRSLGWDKNEIDLLVLVTQSRDYFLPSSGVILQD
                     RIGLPHTCASFDIGLGCSGYVYGMSVVASMLQTGGFRKAILIAGDISTVSLNYKDKSA
                     YPLFGDAVTATGIEFNPGGEASDFNLQSDGGGFEAIIIQDGCVRNPPSDESHIEHEVE
                     KGIIRSRKNLWLNGMDIFNFSVREAPKNITALMNFNGTTEKDYDFFVMHQANLLMNET
                     IRKKLKIPAEKTPYSLKDYGNTSSASIPLTILTQCAEAAQQPRRWLLSGFGVGLSWAS
                     CSLFTDKIICPPVLEL"
     gene            complement(6862..7098)
                     /locus_tag="VFU15_15475"
     CDS             complement(6862..7098)
                     /locus_tag="VFU15_15475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HEU4719244.1"
                     /translation="MDDIKTFIGKLEAELEGLTPGSLTPETNYRQMPEWSSMHALILI
                     ALAETEYNISVTGDDLRSCETINDLYALLKSRTS"
     gene            complement(7145..8149)
                     /locus_tag="VFU15_15480"
     CDS             complement(7145..8149)
                     /locus_tag="VFU15_15480"
                     /inference="COORDINATES: protein motif:HMM:NF006829.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HEU4719245.1"
                     /translation="MKARISAIEYVLPEKIYTNEDFFSEFPGAKAETLLKTGVRKRHI
                     AGKDEISSDLAFRAAEKLITENNIDRSAIDFLLFNSADIDYYTPATACVLQGRLGLKN
                     SCGAMDILNGCSGYVYSLGIATGIMESMGAKNILLLNSSTLTRFIHPRDRANRFLFGD
                     AAAASLITFSETGAIGPFVYGTDGKSFEKIIIRHGFARHPVTQESLTDREDEYGNVTN
                     DASFYMDGVGIFLFSVRTVPPMINELLQKAGQTMDDVDLFVFHQPNAFLNEMLRKKLN
                     IPEEKFVHCMADFGNTVQSTIPIALKEMQKQGRLREGMNVVMTGFGTGLSWAATLARF
                     "
     gene            8353..8751
                     /gene="rpsL"
                     /locus_tag="VFU15_15485"
     CDS             8353..8751
                     /gene="rpsL"
                     /locus_tag="VFU15_15485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002661369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEU4719246.1"
                     /translation="MPTIQQLVRKGRFKAENRSKSAALDSCPQKRGICTRVYTTTPKK
                     PNSALRKVAKVRLTNKIEVIAYIPGEGHNLQEHSIVLVRGGRVKDLPGVRYHIVRGAL
                     DTAGVEGRNQRRSKYGTKKAKGGAKPAAKK"
     gene            8784..9251
                     /gene="rpsG"
                     /locus_tag="VFU15_15490"
     CDS             8784..9251
                     /gene="rpsG"
                     /locus_tag="VFU15_15490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEU4719247.1"
                     /translation="MRKARAKKRPLLPDPKFNDILITRFVNNLMSEGKKSTAYNIFYD
                     AVEIVQKKTEQDGVEVWKKALANVTPNVEVRSRRVGGATFQIPQEIRPERRTALGIKW
                     LISYARVRNEKSMAARLAAEIVSASKEEGAAFKKKEDTHRMAEANKAYSHFRL"
     gene            9277..11400
                     /gene="fusA"
                     /locus_tag="VFU15_15495"
     CDS             9277..11400
                     /gene="fusA"
                     /locus_tag="VFU15_15495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008506300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEU4719248.1"
                     /translation="MSDLKYTRNIGIAAHIDAGKTTCTERILYYAGVNHKIGEVHDGA
                     ATMDWMVQEQERGITITSAATTCHWNYRGNKYKMNIIDTPGHVDFTVEVNRSLRVLDG
                     LVFLFSSVDGVEPQSETNWRLADNYNVTRIGFVNKMDRAGADFLNVVKQVKEILGGNP
                     VPLQLPIGAEENFKGVVDLINNRGIVWNEADQGMTFEVVPIPADMQEDVLHWREKLLE
                     SVSEYDDTIMEKFFEDPASITEAEILTALRKACLANKIVPMVCGSAFKNKGVQTMLDY
                     VMELMPSPLDKENIKGINPDTEEEISRRPDVSEPFTALAFKIATDPFVGRLAYFRCYA
                     GKLDSGSYVLNTRSGQKERISRIFQMHANKQNQVPFIEAGDIGAAVGFKDIKTGDTLC
                     DENSPIVLEAMKFPEPVIGLAVEPKTQADLDKLGVALAKLAEEDPTFRVKSDEETGQT
                     IISGMGELHLEIIVDRLRREFKVEVNQGAPQVNFKEAITGTFEHREVYKKQTGGRGKF
                     ADIKFTVSPVDADFVPDAKNGGLQFVNEITGGNIPREFIPAVEKGFKASMVNGVLAGY
                     PVQTMKIRLTDGSFHAVDSDSLSFEICAKTAYREAVPQARPVLLEPIMKVEVLTPEQN
                     MGDVVGDLNKRRGQIEGMDSRAGVQVVKAKVPLAEMFGYVTQLRTITSGRASSTMEFS
                     HYAEAPRNISEEVIAKANGKKAEKV"
     gene            11425..11733
                     /gene="rpsJ"
                     /locus_tag="VFU15_15500"
     CDS             11425..11733
                     /gene="rpsJ"
                     /locus_tag="VFU15_15500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEU4719249.1"
                     /translation="MSQKIRIKLKSYDYNLVDKSAEKIVKTVKSTGAVVSGPIPLPTH
                     KRIFTVLRSPHVNKKAREQFQLCSYKRLLDIYSSTSKTVDALMKLELPSGVEVEIKVP
                     "
     gene            11768..12385
                     /gene="rplC"
                     /locus_tag="VFU15_15505"
     CDS             11768..12385
                     /gene="rplC"
                     /locus_tag="VFU15_15505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016779062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEU4719250.1"
                     /translation="MSGLIGKKIGMTSFFSAKGKYVPCTIIEAGPCVVTQVKTMEKDG
                     YQALQLAFDEKKEKHTSSAMAGHFKKANTTPKRKLAEFDDFEEAKNMGDVLTVDLFSE
                     GELVDISGISKGKGFQGVVKRHGFSGVGGNTHGQHDRNRAPGSLGNSSFASRVVKGMR
                     MAGRTGGKTIKTQNLEVVKVLPEKNLLIVKGSIAGAKGSYVLIRK"
     gene            12387..>12625
                     /gene="rplD"
                     /locus_tag="VFU15_15510"
     CDS             12387..>12625
                     /gene="rplD"
                     /locus_tag="VFU15_15510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010262380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEU4719251.1"
                     /translation="MELSVYNSEGKATSKKVSLNDAVFGIEPNDHAIYLDVKQYLASQ
                     RQGTHKSKERAEINRTTKKLKRQKGTGGARAGSMKS"
BASE COUNT         3031 a         3228 c         3318 g         3048 t
ORIGIN      
        1 ccggtttttt gcaagccctg cttattcatc cccatttttt taccttcatg ctccgatggc
       61 gacatttgcg catatcatca atcccgtaag ggcccccgcg ggatcggaac tcgacattgt
      121 gcaacccgcc acgtttgcga cggtgaaacg cgcgctcggt gaagcggaag gaaaagtgca
      181 ggtggatttt ctttcggcgc agtttcccga agaccatccc gtcatccctt cctgggtgaa
      241 aaaaacaccc gacctcgagc gttctcttgc tgatctcgga tcattcgcaa agccgcggcg
      301 gctccccgtg ctccgtgata ttctcgaccg cgcaatgcag gcgtctgatg cggattatat
      361 cattttctcg aacgcggata tctgcctcat gccctcgttc tatctcgtgc tcaatgaata
      421 tgcggagaaa ggatacgatg cgttcgcgat caaccggaga aggatccctg cccgttttgc
      481 gggcgaaaca agaatggagc tgctctacgc cgaggccggt gaaaaacatc cgggttacga
      541 cacgcttgtg ttcaggcgct cgctgtatga aaaattcgta ttgggaaatg tctgcaccgg
      601 tatcccgcgt ttcgatacga tactcattca taatttttac gctttcgcgg aaaatttccg
      661 tctcttcacc gggaagcatc ttacgtttca catcggcatg acgctttgca aacgatgggg
      721 cgataacgac cagtatgcgt tcaacgaaaa agaatatgcg atggtgcgca ggaagctgta
      781 cccgcatttc cgcatcgaaa atttccccgg ggccaatctt ccttttctcc gcaggcattt
      841 caaatggttc ctcaacccga ccttcagcta cccgatgatg ttccggctcg atctttccca
      901 gctttcaagg ccgagaagaa aaagggaaaa gaaagagatc tgcggcctgc aaaaccgctg
      961 gtgcgaattc ggaagccgtt tcatcaattt cgacgacgaa tactgatcgt tacggagtaa
     1021 gtgcacgcgg ccaccagccc aggtaacggt tcatcagctg gttttttctt ttgaactttt
     1081 ccacgcgtgc ttcgaaggcg ggaccttttt tccggagctc ctccctgact ttcggcgcga
     1141 tgatgtcgta gagtttttga tccagcacgt tgcaggaacg tacggcctca agtgtttccg
     1201 catcgaggtc ctgcacgcgc ggacgggaaa cgcctgtccg ctggcggcgg taatacagga
     1261 attttttcca gtgataaagg tcggcgaggc tgagcacgaa cgcatcgaaa ttttcctgca
     1321 ggccggtcag tgtaaaatac ttttcaagat tttccagcgc ggtgtccagc atttcttccg
     1381 tgcattctcc gaacggaaca tcgtccttcc cggaaatgac gcgcaccatg taattgtcga
     1441 ggtggatcat catcctgttg cgtatcacgt ggagaagcgt gtagccgttg cggttgtatt
     1501 cctcatgcag cggatgcgtt ttgaaatcga gtatgaaatg gaattgggaa acggtccgct
     1561 ccagcggatc gcgcaggata gtgatgtagt cgcatttccc gggaaaataa cggtgaagcc
     1621 cgaaggaaaa atgcccgttc acaagacgaa tgttctttcg ctcctcttcg ctcagcgaag
     1681 cggtgatttt ttccgccgtg cgcggatcgt aaagattcag cgtcctgcgt ttttcatact
     1741 gccgtgtgat gatgttgagc agggtagtac ccgctgtttt ctggatgtgc aggaacagca
     1801 gcgtcttttc ggtatcgggt gcggcgctca aaaatagatg cgtgaagaaa acacgttcag
     1861 caaatgtaat ctttcgggtt acgtgtcgtc agggtgtcag gaaatcgggc caccagccga
     1921 gcacgcggtt gagcagctgg ttcttttttt tgaagctcgc cagccgggca ttgaaacggg
     1981 cgccctggcg cgcaatgcgg tcctcgatga tcggcgacca cgtatcgtaa agtttctggt
     2041 cgagcgtatt gtgcgcgcgc agggcatcca ttgttttttt gtcgatctct tcggttttcg
     2101 gccggcttcg cccggcgttc cgcttgcggt aaaaaatata cccgttccag tgaaaccgtt
     2161 cgctgcacat aagcacgaac tcatcgaaat tcttcacgcg cccgacgacg gaaaaattct
     2221 tttccaggtg cgtcatcgcg tcgtccagca tggtttccgt gcaggcgccg tacggcgcgc
     2281 tgtcatttcc cgaaagcatc cgtaccattt tgttgtcgag gcgcggaatg gctccgttct
     2341 cgagcacttc caggagggaa taaccgctgt ccacgatcat gtcgtggtaa ttgtgcgtga
     2401 cggaatcgat cacgaaatga aaatgcgaga tggtccgttc gaccgggtcg cgcagcattg
     2461 tgatgtattc gcagggctcc ctgaaaaaac ggtgcgcccc gaatgcgaag tggcctttca
     2521 gcagccggat ctgcgacgct ctttccggtg tgaggcgttt caattccgcc aggcccttct
     2581 ccgtgtggta aatgctcagt gtgttttttt tcgggtactg tcgcccgatg atgttatgca
     2641 tcgtcacgcc ggccgtcttc tgtatatgaa gaaagagcag gtggggagcg acaggaaggt
     2701 tcggttcagt attcattttt gaaacggaag taatgtattc ggaagaaaaa tcgccgcaca
     2761 aggccgcgga tctttccggg aaatttccgg aacaacggga acgcctgctc gagcctttgc
     2821 agcgaaagca tcgaccgcac attcgagcat atttcacgtt ttcgcgattc gtacccggga
     2881 cgcagaacgt cgacgatcag cacgatccgc gtctgtttcg tatggttcca tgcgcggtga
     2941 taatgcgcat cgcagaaaag cagccacttc cctttttccc acgaacgctg ctcgccgttc
     3001 acttccagtc cgcattccgg gagcggcgca gggatcacca gcccgagatg acagcgcatc
     3061 accgcatccg tgtcgccgaa gtgcggaaga atatccgttt ccgccttcag caggctcagt
     3121 ccgccggaaa gcatgcccgg gaccgaccaa ataaaatcgt cgatcgccgg cacagcgcgg
     3181 cacagctctt cgtttttaag gccccagaaa tagaatgttc cctgccgcca cgatcctttt
     3241 ttcgaaacga ggttcgcatt gaaatacggc gccgaacgtt cctcgaagct gccgataaat
     3301 cccttcgccg cttccatcag cccgggaaag cgggattctg tttccgccac ccacgcgaaa
     3361 gcggaaccat cgtagaacga cggggcgttc ccgggatagc ttccgccgcg gaatgaatac
     3421 cagggatgcg tgcttctgct catcggccgg gagtgatcag ttctaagtgc gcaaggacct
     3481 gctccgggag ggcccggatc ttttcctcca ggaaacgggt cacaggatgt tcgggattgt
     3541 cgaagatgat cgtcatccgg tctccccatt tccggtgaaa gatttctttg atcttttttg
     3601 ttttcgcctc gtaattttcc tgcgtgagaa catactcctg gttgaccgaa ttctcctgtc
     3661 ccggaagcaa ttcataaccg aggttatgat tatacgagat cgcagccgcg ttatcacgca
     3721 gaatttttac gtaggtacgt tccagtccca ggtaaaacga cgtgtcggtg agaagcagcg
     3781 atgccgcaag cggaatttcc gtccgcagga cttcgtcatc ccagataaag atgccgcctg
     3841 agccggtttc tttttttttc cagttcactt tgcagccgtt gatcaggccc acctcttttt
     3901 cccccgaaga aatgatgtaa taatgatttt cggtattgtc aatggaacgg aaccattttt
     3961 cctgcatttc aggcgtgatg tattcccggt attccatgtg gcgcacaatt ttttccatat
     4021 tgcgcatctg cctcaccagt tcgatctttt cgtgcgtaag cctttccagc ctgacgttgt
     4081 aaccggtaag aatcatgccc taagtaaaca aaaagaatgc gttccgggaa acgaaagcgg
     4141 tcaaacaact ttatccacgg tgaacggggg acgcctgttc cgcgtctggt aaagcttata
     4201 catgtattgc ccgatgatgc cgaggcaaag catgagcacg ctggtggaaa aaagtacggt
     4261 aacgatgatg gcggtaaatc ccggcgccga tccgtgaacg agctttttgt aaatgaaacg
     4321 gagaccgagc atgaatgtca ggcaggagct gatcagtccc acccatgtca tcgccttcag
     4381 cggaaacgcg gaatagttga tgatgatgtc gaagtaaaag gagatgagct ttcctcctga
     4441 ataggtcgat tttcccgaac gccgcgggtg gtgttcgaca ttgatataag aaacgttccc
     4501 gctgtaccag ccgatgatct cgtcgatgaa aataaacccg gtgcggttgt gctgaatgat
     4561 cttttcggca atggattttg aaaggatgcg gaaggagctt ccccttcccg gaccttctcc
     4621 cattgtcttc gaaccgcgtc tcagcagcca gctcccggtt tttctccacc cgggatgttt
     4681 tttgttcggg taatcgccgt aaacaacgtc gcacctgctt ctttcatatt cggcgatcag
     4741 cttgggtatc tccgaaggcg gatgctgcag atcatcgtcc atcgtaatga tcgcatctcc
     4801 cttggcaaac ccgagcccgc agctgatcgc gttatgctgg ccggaatttt tcagcaggct
     4861 tattcccgtg acgtgatcgg gatttgcatc tcttattttc tgaatgaccg cgaagctttc
     4921 gtcgcggctc ctgtcattca cgaagatcac ttcgaacgga acaacgccgc tcacagccga
     4981 agaaatttct tcgtacagct gttccagcat ttgtgcgctg ttgtaaacgg gaatgacgat
     5041 ggaaagaaac tggatgcgca tattatcctc cggataaaaa tcctccgtcc aaaataatgt
     5101 ttattcccgt cacccaccgc gatgccggcg agagcaggta tatggccgca ttggcaatat
     5161 cttcggggag tcccgcgcca agcgggtagc ctttcatgtg ctcgtccatt tcagcttttg
     5221 aaacagccgc ctcggcccgg tcgtacagcg gcgtttttac catgcccggt gaaatggaat
     5281 tcgcacgcat gccggaagaa acaatttcct gcgcaagcgt tttgacgaat gtttcgagcg
     5341 ctgccttggc gctgccgtac atggatccgc ccttcggcgg gtatttggag gagatggaag
     5401 aaagatagac gagcgaagcg tcgcgcttta atttcttgtt cttcagcagg ccggcggtga
     5461 ggagcacggc tgcgtcgaaa ttcagcttca tcatctcgtc gatgcgcacc tggtcgagga
     5521 agcgtatggg gaacgctttt acatttcccg cgcagtgaac caggccgtct gttgccggca
     5581 attgttcaag gaggcgcttt cttcctgttt cgtcaagaag atcggcggca atggaaatat
     5641 gccccttccc ttcgagtgat cccaatgttt ctgaaagcgc ttcttcattg cggccgctga
     5701 tgatgcaggt tccgcccatc cggctgatgc tgatcgccac ttgtctcccg atgcccgacg
     5761 aagcgccggt gacaagaatg attttgtcgt caagatggaa gggtgttttc atagttcgag
     5821 aacgggcggg caaatgatct tgtcggtgaa aagagagcag gaggcccagc tgagacccac
     5881 accgaagccg gaaagcagcc accgccgcgg ttgctgcgca gcttccgcgc attgcgtcag
     5941 gatcgtaagc gggatggagg cggagcttgt gttgccgtaa tccttcaggg aatacggcgt
     6001 tttttccgcg ggtatcttca gcttcttgcg tatcgtttcg ttcatgagca ggttcgcctg
     6061 gtgcatcacg aaaaaatcat agtctttctc ggtggtcccg ttgaaattca taagcgcggt
     6121 tatgtttttc ggcgcttcgc ggacggaaaa attgaaaatg tccatcccgt tcagccagag
     6181 atttttccgg ctgcggataa ttcctttttc cacttcgtgc tcgatgtgcg attcatcgct
     6241 cggcggattt cgtacgcagc cgtcctggat aatgatcgct tcgaatccgc ccccatcgct
     6301 ttgcaggttg aaatcggaag cttccccgcc gggattgaat tcgattcctg ttgcagtcac
     6361 cgcgtcgccg aaaagcgggt aggcgctttt gtctttgtaa ttgagcgata cggtggaaat
     6421 gtctcccgcg atgaggatcg ctttgcggaa tcctccggtc tggagcattg acgcaactac
     6481 cgacattccg taaacatatc cgctgcatcc caggccgatg tcgaatgagg cgcacgtatg
     6541 cggaagcccg atcctgtcct gcagaataac gcccgaagaa ggaaggaagt aatcccgcga
     6601 ttgcgtcacg agcaccagca ggtcgatctc gttcttgtcc catccgagag agcggatcag
     6661 cagctcggcc gccgggacgg cgagatcggt tgtggtcgtc ccttttccgg caatgtgccg
     6721 ggaggaaatt cccgtggtct tgatcaggag gtttctttcc gcttcggaaa tccaatcgta
     6781 atcgcggtta tattccttcg ctgaaggaac acatgctgca attcctttta cctgggttcc
     6841 gcggacagaa aaaatggcca ttcaggaggt gcggcttttg agaagcgcgt aaaggtcgtt
     6901 aatggtttca cacgagcgga gatcgtcccc ggtaacagaa atattgtatt cggtttccgc
     6961 cagtgcgata agaatcaggg catgcattga gctccattcg ggcatctgcc ggtaattggt
     7021 ttccggtgtc aggcttccgg gcgttaaacc ttccagttct gcttcgagtt tgccgatgaa
     7081 ggtctttatg tcgtccataa aatgagttga atgaattcaa aaataggtat tccgccggaa
     7141 cgtatcagaa acgggccagg gttgcagccc acgacagacc cgtaccgaaa ccggtcatca
     7201 ccacgttcat gccctcgcgc aggcgcccct gcttctgcat ttctttcagc gcaatgggaa
     7261 tggtcgattg caccgtgttg ccgaaatcgg ccatgcagtg aacgaatttt tcttccggga
     7321 tattcagctt tttccgcagc atttcattga ggaatgcatt gggctggtgg aaaacgaaaa
     7381 ggtcgacgtc gtccatggtt tgtcccgctt tctgaagcag ctcattgatc atgggcggca
     7441 ccgtgcggac agaaaaaaga aatattccca ccccgtccat gtaaaagctg gcgtcgttgg
     7501 ttacgttccc gtattcatcc tcacggtcgg tcagcgattc ctgcgttacc gggtgacggg
     7561 caaatccatg acggataatg atcttttcga aactcttgcc gtctgttccg taaacgaacg
     7621 ggcctatcgc tcccgtttct gagaacgtga tcaggctggc agccgcggca tcgccgaaaa
     7681 ggaaacggtt ggccctgtcc ctggggtgaa tgaaacgcgt gagcgtcgag gaattcagga
     7741 gaaggatgtt tttcgccccc atgctctcca ttattccggt ggcaatcccc aggctgtata
     7801 catagccgct gcacccgttg aggatgtcca tcgccccgca tgaatttttc agcccgagtc
     7861 ttccctgcag gacgcacgcc gttgccgggg tataataatc gatatccgca gaattaaaga
     7921 gcaggaaatc gattgcggaa cggtcgatgt tgttttccgt gatcagtttt tcagcggcac
     7981 ggaacgccag gtcggaagaa atttcatcct ttcccgcaat gtggcgtttc ctgaccccgg
     8041 ttttcaggag ggtttctgcc ttcgcacccg ggaattcgct gaagaaatcc tcgttcgtat
     8101 agattttttc cggcaaaacg tattctatcg cacttattcg ggctttcatc agctcaaagg
     8161 taacaaacag aaccggtgac gtcccggatg ccgcgccgga gcctgttttc gggggctgaa
     8221 aacagtgaaa aattattttt gcaccatgtt gtttataaag aagaatttgt acctttgccc
     8281 tccccaaaat ccggactggg gtagtaattt tttgtataaa acgttgaaaa acaattaatc
     8341 gcgaataaaa tcatgccaac aatccaacag ctcgtacgca agggtcgttt caaggctgaa
     8401 aaccgcagca aatctgctgc tctcgacagc tgcccgcaga agaggggaat ctgcacgcgc
     8461 gtttatacca caacgccgaa aaagccgaac tccgctctcc gtaaagtggc aaaggtgcgc
     8521 ctcacgaaca agatcgaggt gatcgcctat atcccgggtg aaggacacaa cctgcaggag
     8581 cactcgattg ttcttgttcg cggtggtcgt gtaaaggacc tgccgggcgt ccgttaccat
     8641 attgtacgtg gtgcgcttga tacggccggt gtggaaggcc gcaaccagcg tcgttccaag
     8701 tacggcacca agaaagctaa aggcggtgca aaacccgcag ctaaaaaata attcagaaaa
     8761 cgaactgttc atcctgagat aaaatgagga aagcaagagc aaaaaaacgg ccccttctgc
     8821 ctgatcccaa gttcaacgac atccttatca caagattcgt gaacaacctt atgtccgagg
     8881 gaaaaaaatc caccgcatac aacattttct atgacgcggt ggaaattgtt cagaagaaaa
     8941 ctgagcagga cggcgtggaa gtatggaaaa aggccctcgc caacgttacg cctaatgtgg
     9001 aagtccgcag ccgccgtgtg ggtggtgcta ctttccagat ccctcaggag atccgcccgg
     9061 aacgcagaac tgcgctcggc atcaaatggc tgatcagcta cgcgcgcgta cgcaatgaga
     9121 aatccatggc tgcccgcctt gctgccgaga tcgtttcggc ttccaaagag gaaggtgcag
     9181 cgttcaaaaa gaaagaagat actcacagaa tggccgaagc gaacaaggcc tattcgcatt
     9241 tccgactgta atcaaccaaa gaagaagaga gacgtcatga gcgaccttaa gtatacaagg
     9301 aatatcggga ttgcagccca catcgatgct ggtaagacaa cctgtaccga gcgcattctg
     9361 tattatgcag gtgtgaacca caagatcggt gaggtgcacg atggcgccgc aaccatggac
     9421 tggatggtgc aggagcagga gcgtggtatc acaattacct ccgctgccac aacctgccac
     9481 tggaattacc gcggaaataa gtacaagatg aatatcattg acacgccggg tcacgttgac
     9541 ttcacggtag aggtcaatcg ttctctgcgc gtcctggatg gcctcgtatt ccttttctct
     9601 tctgttgatg gcgtcgaacc ccagtctgaa accaactggc gtctcgccga taattataat
     9661 gttacccgca tcggattcgt aaataagatg gatcgtgcgg gtgctgattt tctgaatgtc
     9721 gtcaagcagg taaaggagat tctcggcggt aacccggttc cgcttcagct cccgatcggc
     9781 gctgaggaga atttcaaagg tgttgttgat cttatcaata accgcggcat cgtttggaac
     9841 gaggctgacc aggggatgac gtttgaagtc gttccgattc ctgctgatat gcaggaggat
     9901 gttctgcact ggcgtgaaaa gcttcttgaa tctgtgtccg aatacgacga cacgatcatg
     9961 gagaagtttt tcgaagatcc ggcttccatt accgaagctg aaattcttac ggcgctccgc
    10021 aaagcgtgcc ttgcaaacaa gatcgttccg atggtttgcg gatccgcttt caaaaacaaa
    10081 ggcgttcaga cgatgctcga ttacgtgatg gaactgatgc cttctcctct cgacaaggaa
    10141 aatatcaaag gcatcaatcc cgacacggaa gaggaaattt cccgcaggcc tgatgtgagc
    10201 gaacccttta ctgcgctcgc attcaaaatt gccactgatc ctttcgtagg ccgccttgct
    10261 tatttccgct gctatgcggg aaagctggac tccggctcct atgtgctcaa cacgcgctcc
    10321 ggacagaaag aacggatctc gcgtattttc cagatgcatg ccaacaagca gaaccaggtt
    10381 ccgttcattg aagcgggtga tattggcgcc gcggtaggat ttaaggatat taagaccggc
    10441 gatacgctgt gtgatgaaaa ttcacccatc gttctcgaag ccatgaaatt ccctgagccg
    10501 gttatcggtc tcgctgttga gcccaaaacc caggccgacc tggataaact gggtgttgcg
    10561 cttgcaaagc tggcggaaga ggatcctact ttccgtgtga aatcggatga agaaaccggc
    10621 cagacgatca tcagcggtat gggcgagctt caccttgaga ttattgtcga ccgccttcgc
    10681 cgcgaattca aagtggaagt gaaccagggc gcaccgcagg ttaacttcaa agaagcaatc
    10741 accggtactt ttgagcaccg cgaggtctat aaaaaacaaa ctggtggtcg tggtaaattc
    10801 gcggatatca aattcactgt ttccccggtt gatgcggatt tcgttccgga tgccaaaaat
    10861 ggcggactcc agttcgtcaa tgaaattacc ggcggaaata ttccccgcga attcattccc
    10921 gctgttgaaa aaggtttcaa agcatctatg gtcaatggcg ttcttgcagg ctatcctgtg
    10981 cagacgatga agatccgtct tacggatggt tctttccacg cggttgactc tgattccctt
    11041 tcattcgaga tctgcgccaa aacagcctat cgtgaagctg ttccgcaggc aagaccggtt
    11101 cttctcgagc cgatcatgaa ggtggaggtg cttaccccgg aacagaacat gggtgatgtt
    11161 gtgggcgatc ttaacaaacg ccgcggccag atcgaaggta tggattcgag agccggtgtg
    11221 caggtggtaa aagcgaaagt gccgcttgcg gaaatgtttg gttacgtaac gcagctccgg
    11281 accattactt ccggtcgcgc ttcttccacc atggaattct cgcattatgc ggaagctccg
    11341 agaaacattt ccgaggaagt gatcgcaaaa gcaaacggca agaaggccga aaaagtttaa
    11401 tcatcatccg ataagagcat aaacatgagc cagaaaatcc ggataaaact caaatcgtac
    11461 gactacaacc tcgttgataa gtcggcagag aagatcgtga aaaccgtaaa aagcacggga
    11521 gctgttgtca gcggacctat tccgctgcct acgcacaaac gcatttttac cgttcttcgt
    11581 tctcctcacg taaacaaaaa agcacgtgag cagttccagc tctgcagcta taagcggctc
    11641 ctggatattt acagctcgac atccaaaacg gtggatgcgc ttatgaagct tgagcttccg
    11701 agcggtgtag aagttgaaat caaggttccg taaccggaaa ccagtttaaa taaatcatac
    11761 agaaacaatg tcaggcttaa ttggtaaaaa aatcggaatg accagcttct tcagtgccaa
    11821 gggtaagtat gtaccatgca caattatcga agcaggtcct tgcgtggtga cgcaagtaaa
    11881 aaccatggag aaggacggct accaggccct tcagctcgcc tttgacgaaa aaaaggaaaa
    11941 acacacgtct tccgcaatgg cgggtcattt caaaaaagcg aatacgactc ccaagcgcaa
    12001 gctggccgaa ttcgatgact tcgaagaagc aaagaacatg ggtgatgtgc tcaccgttga
    12061 tcttttttca gaaggagaac tggttgatat atcaggaatt tccaaaggaa aaggtttcca
    12121 gggtgttgta aagcgtcacg gtttcagtgg tgtgggtggt aacacgcacg gccagcatga
    12181 ccgtaaccgc gcgcccggtt cgctgggtaa ctcttcgttc gcatcacgtg tcgtaaaagg
    12241 catgcgcatg gcaggaagga caggtgggaa gaccatcaaa acccagaatc tcgaagtagt
    12301 aaaagtgctt ccggaaaaga atctcctcat tgtgaaaggt tctattgccg gcgcaaaagg
    12361 ttcatacgtt ctgatccgca aataacatgg aactttcagt atacaactca gagggaaaag
    12421 cgacctcgaa aaaggtcagc ctgaacgatg cggttttcgg tattgaaccg aatgatcacg
    12481 ccatctatct tgatgtgaag caatacctcg cttcgcagcg ccagggcacg cataaatcaa
    12541 aagagcgtgc tgaaatcaac cgcacaacga aaaagctgaa gcgccagaag ggaaccggcg
    12601 gtgcgcgtgc cggcagcatg aagtc
//
LOCUS       DASTSF010000542         2279 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1468680,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000542 DASTSF010000000
VERSION     DASTSF010000542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2279
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1468680"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(149..1162)
                     /locus_tag="VFU15_15515"
     CDS             complement(149..1162)
                     /locus_tag="VFU15_15515"
                     /inference="COORDINATES: protein motif:HMM:NF022732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3089 domain-containing protein"
                     /protein_id="HEU4719252.1"
                     /translation="MKFIRPKHLPLLFLLAGISCIHPRHAYDHYPKPPAPDYSKPECW
                     ASLPSLKDWADSVPANTNLENGEANAQVDVFFIHPTSDHSRDNWNGDVYNAALNHETD
                     HGTIRLQCSVFNESCRIYAPRYRQATLASFTDSVNGPKAIDFAYEDVKTAFEYYLAHY
                     NNGRPFIIASHSQGTRHAVRLLHEFIDNDPVLRKKLVAAYLIGQDVKNDEYKNIPPAD
                     SAAQTGCVISWRSEEWGYVPPKGKSFKNMLCVNPLTWTRKTDYAGKEKNHGSVPTMFD
                     RIDAGIADAQIVDGMLWVHKPGKPGYPPFFKTYHLVDYNLFYINLRENIKLRVENYLS
                     QQE"
     gene            complement(1233..1895)
                     /locus_tag="VFU15_15520"
     CDS             complement(1233..1895)
                     /locus_tag="VFU15_15520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719253.1"
                     /translation="MSKAKTKRIFSDLFNTDYSILKVKYNVRADEAPADVQIKNTLGK
                     KAGTNSITELARICTSPKLKDFVDFYSKHDGFDLATPKSPRNVVKKTLLRQIPASDLE
                     KFTSHYLPGGRWAWTIDLNKTKALYRNENKWLAFAEVNGGPACLTIFLDGENAGNVFL
                     LNAQPHFNILKPIARTYAGLLDRIAKDPAAFFKLIKAYVVLPGENGQNYGHVPLEYVD
                     KN"
     gene            complement(1989..>2279)
                     /locus_tag="VFU15_15525"
     CDS             complement(1989..>2279)
                     /locus_tag="VFU15_15525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HEU4719254.1"
                     /translation="LINEGRMRPAGLKVVEKAKADGSWERAYDSPGKMTIPGDFLKEL
                     SKNKKAEAFFAGLNKTNLFSIGFRLQTAKKQETRKKRMKEIIEMLAKGRKFH"
BASE COUNT          494 a          546 c          569 g          670 t
ORIGIN      
        1 aacgatctgt atggatttcc gcttcatgtt attctgtttt tatcaccagg aaacaaaata
       61 aaaacatgga aacggcagaa taaatgatca tcgtggctgt tttgcgtgat gatcgtcacg
      121 ctgcggccat tcggggaaaa aactccttct attcctgctg cgaaagatag ttctccaccc
      181 gcagctttat gttctcgcgc aggttgatat agaaaagatt gtaatcgacg aggtggtacg
      241 ttttgaagaa gggcgggtag cccggctttc ccggtttgtg cacccacaac atcccgtcga
      301 cgatctgtgc gtcggcgatg ccggcgtcaa tgcggtcgaa catggtcgga acgctcccgt
      361 ggtttttttc ttttcccgcg taatccgttt tgcgcgtcca cgtgagcggg ttcacgcaga
      421 gcatgttttt aaaacttttt cctttcggcg gaacgtagcc ccactcttcc gaacgccacg
      481 agatcacgca accggtctgc gcagcggaat cggccggcgg aatgtttttg tattcgtcgt
      541 tcttcacgtc ctggccgatg aggtaagcgg ccaccagttt tttccgcagc acgggatcgt
      601 tgtcgataaa ctcgtgcagg aggcggacgg cgtgacgcgt gccctggctg tgcgaggcga
      661 tgatgaacgg gcgcccgttg ttgtaatgcg cgaggtaata ttcgaacgcg gtcttcacgt
      721 cttcataagc gaaatcgatg gctttcggtc cgttcaccga atctgtaaag gatgcgagcg
      781 tcgcctgccg gtagcgcggc gcatagatgc ggcacgactc attgaagacg ctgcactgca
      841 ggcgtatcgt cccgtgatcg gtttcgtggt tgagcgcggc attgtacacg tcgccgttcc
      901 agttgtccct gctgtgatcg gaagtaggat gaataaagaa cacatccacc tgcgcattgg
      961 cttccccgtt ctccaggttg gtgttcgccg ggacagaatc ggcccagtct ttcagcgacg
     1021 gcagcgacgc ccagcattcc ggcttgctgt aatccggcgc gggcggcttc ggataatgat
     1081 cgtacgcatg tctcgggtga atgcaggaaa ttcccgcgag gaggaaaagg agcggaagat
     1141 gtttcggacg gatgaatttc attcgcgcaa tttacggatt gcagctttgc tttgaccggc
     1201 tttcctgccg gcaggccggc ggccgtactc tcttaattct tgtcaacgta ttcaagaggt
     1261 acgtgtccat aattctgtcc gttctcacca ggcaatacca catacgcctt tatcagctta
     1321 aaaaaggctg ccggatcttt tgcaattcta tccaggagac cggcgtatgt cctggcaatc
     1381 ggtttcagaa tattaaaatg tggttgtgca tttaataaaa aaacatttcc ggcgttttct
     1441 ccgtcaagaa aaattgtgag acatgccggg ccgccattaa cttctgcaaa agccagccat
     1501 ttattttcgt tgcgataaag agctttggtc ttattcaagt ctattgtcca tgcccatcgc
     1561 ccgccaggta aatagtggct tgtaaatttt tccaggtctg aagcgggtat ttgcctgaga
     1621 agtgtttttt taacaacatt ccggggagac tttggagtcg ccagatcaaa tccgtcatgc
     1681 ttagaataaa agtcaacgaa gtcttttagt ttaggggaag tgcagattcg cgccagttcg
     1741 gttatgctgt ttgtgcctgc ctttttacca agtgtatttt taatctgaac gtctgccggc
     1801 gcttcatctg cgcgaacatt gtattttact ttcaatattg aataatcagt attgaacagg
     1861 tcggaaaaaa ttctttttgt ttttgcctta ctcattttgt ggtttattcc gggcacgaaa
     1921 tcccgcgtat tgcaaaggtg ttctgagcag ctgctacttt ccaggtaatt gcttttccgc
     1981 cggattattt aatggaactt cctgcctttt gccagcattt caataatttc tttcatgcgc
     2041 ttttttcttg tctcttgttt ctttgcggtc tgaaggcgaa atccaatcga gaaaagattc
     2101 gtcttattga gaccggcaaa aaacgcttcc gcttttttgt ttttgctaag ttctttcaga
     2161 aagtctccag gtatagtcat tttgcctggg gaatcgtaag ccctttccca gctaccgtct
     2221 gctttggctt tttcgacaac ttttaatcct gcgggtctca tcctgccttc attgatcaa
//
LOCUS       DASTSF010000543         4063 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1500034,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000543 DASTSF010000000
VERSION     DASTSF010000543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4063
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1500034"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..915
                     /locus_tag="VFU15_15530"
     CDS             <1..915
                     /locus_tag="VFU15_15530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719255.1"
                     /translation="PALVFHSCNGKKQQVKKDPGERPLKECGTAVFSSLSQTGWLFLD
                     STSEEYHDSSGMGATLSYPFIGKYPKEFALSSGGSADGSQYELYRLTKRGRYLRNTFF
                     FCWHGFIEIDRRKTNGYNDILEYADHDGNGKLYSMVLRYSFDGNKYVPAYVDSVDGRK
                     MDIPVALLAVAGYLFEPDGSGAYEMRNDSSVFSLENSTYGTVTIAPGSKAAILSSADE
                     WGNNDWFVFINENGEWKTVLSAHGSRCSLKKEKTMTQNHYDFSFVFHDFVDSLNNPGL
                     KYTWKNDHYELNRPADLSGELHFNDSAE"
     gene            1046..3481
                     /locus_tag="VFU15_15535"
     CDS             1046..3481
                     /locus_tag="VFU15_15535"
                     /inference="COORDINATES: protein motif:HMM:NF024220.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HYR domain-containing protein"
                     /protein_id="HEU4719256.1"
                     /translation="MKKTTSLFLFSLFAATASFAQCISINCAGNVSANNDNASCGAVV
                     NYSAPSVVNNCGVDTFLYTGAVQYYTVPAGITTLTIEAWGAQGGANWVNNTNYGGYAK
                     ADFSVTPGEVLEVRVGQQPNGITGGYNGGGNGEGAGQGGGGASDVRQGGSTLNDRIIV
                     GGGGGGAGYWSSLEVVGGVGGGTNGGDGYRVPDYATNPGGLGASQTGPGANGTCVNFY
                     VTAMAGSFGQGGAPSGCGCEGYGGGGGWYGGAGSGNCRGGGGGSGYIIALASNQVATA
                     GVRAGNGMVVISTGQSSGATVTQLAGLASGSTFPVGITTNTFAVSDVFGNADTCSFTV
                     TVTDVENPVISNTPSSISVNNDAGLCGAVVSWTPPTATDNCSATLTSNYNSGDLFPPG
                     TTTVTYTATDPSGNTATSSFTVTVTDTEAPVLSCPADLSISTDSGSCVATGVILGNAN
                     ATDNCSVAGVVNDAPSSFMQGTTVVTWTATDVNGNSTTCTQNVTVTDMEAPSLVCPSD
                     ITVSVDPGSCDATNVLLGTETASDNCAIATVVNDAPNSFPAGTTSVTWTASDAAGNST
                     TCTQLVTVVDSEPPVISCTSDTTICSGVYVFNAPVASDNCSATVTQIAGPNSGDSLAA
                     GNYTLSFSAVDPSGNMAMCSYQVTVNPSPSVSVSSNPTTICVDDAPAALSGTPAGGTW
                     SGPGVTGSTFDPQAAGDGAHTLTYSVTGANSCTGSASFTITVDPCTGIKENTQTLFAM
                     TPNPADQVVSFTLSGSGTLEIMDATGKLVLSKTISGDRYDQGVGDFADGVYTVRFTSN
                     EGKIQVAKLMINH"
     gene            3674..>4063
                     /locus_tag="VFU15_15540"
     CDS             3674..>4063
                     /locus_tag="VFU15_15540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719257.1"
                     /translation="MKKRLLAILIACIPLAGWSQNVFTDFQDGKIWFQVKDDYRINAP
                     LQEDPHHLPIGTLGFLNGISKNHQLTNLSRPFYAAKNSPILQRTFLLEFSDAANIMSV
                     ISELKATGNVVYAERVPLDHECLTPNDP"
BASE COUNT          948 a         1086 c         1096 g          933 t
ORIGIN      
        1 cccgcgcttg tttttcattc ctgtaacggg aagaaacagc aggtgaaaaa agatccgggt
       61 gaaagaccgc tgaaggaatg cgggaccgct gtcttttcat cgctttcgca aacaggatgg
      121 cttttcctgg attccacttc cgaggaatac catgacagtt cgggaatggg agcgacgctg
      181 tcctatcctt tcatcgggaa atacccgaag gaatttgcct tgtcgtcggg tggaagcgcg
      241 gacggcagcc agtatgagct ctaccggctg acgaaacgcg gacgatatct ccgcaacacg
      301 ttctttttct gctggcacgg attcattgag atcgaccggc ggaaaaccaa cgggtataac
      361 gacatcctgg aatacgctga tcacgatggg aacggaaaac tgtattcaat ggtgctgcgt
      421 tacagctttg acggaaataa atatgttccc gcgtacgtgg attccgtgga tggacgcaaa
      481 atggatattc ctgttgccct gctcgctgtc gcgggttacc tgttcgaacc cgatggaagc
      541 ggcgcttatg aaatgaggaa tgactcgtca gtcttttcat tggagaacag cacatacggt
      601 actgtgacga tcgctcccgg aagtaaagcg gccatactct cctccgccga tgaatggggc
      661 aataacgatt ggttcgtttt tataaatgag aacggagaat ggaaaacggt gctgagcgcg
      721 catggttccc gctgttcgct gaaaaaagaa aaaactatga cgcaaaacca ttatgatttt
      781 tcatttgtct tccacgactt cgtcgattca ctgaacaacc ccgggttgaa atacacctgg
      841 aaaaacgatc attacgaatt gaaccgcccg gccgacctgt cgggtgaatt gcattttaac
      901 gattcggcag agtaggtgca ggaaaatgac ttcgtcgttt tttattttca gttccgtgac
      961 gaaaagcggg tgtgcgtcga aagagcgcat gcctgacggg gaaacttgtt aacttgaaga
     1021 aatttttgac caaaatctga aattcatgaa aaaaactacc tcgctttttc tcttctctct
     1081 ttttgcagca acggcttcct tcgcgcaatg catcagcata aactgcgccg gtaacgtcag
     1141 cgccaataac gataatgcct cgtgcggtgc ggtggtgaat tattctgcgc cttcggtagt
     1201 caacaactgc ggcgtcgata catttctgta cacgggcgcc gttcaatatt ataccgtccc
     1261 ggccggcatc accacgctga ccatagaagc gtggggcgcg cagggcggag cgaactgggt
     1321 gaacaacacg aattacggcg gttatgccaa agccgatttc agcgtgacgc ccggtgaagt
     1381 tctcgaagta cgcgtgggac agcaacccaa cggaattaca ggtggatata acggcggcgg
     1441 aaacggagaa ggtgccgggc agggcggggg cggagcatcc gatgtacgcc agggcggatc
     1501 gacgctcaac gacaggatca ttgtcggcgg cggtggcggt ggcgcaggtt actggtcttc
     1561 gctggaagtc gtcggtggcg tgggcggcgg aacgaacggc ggcgacggtt atcgtgttcc
     1621 cgattacgca accaaccctg gtggtcttgg tgccagccag accggccccg gagcaaacgg
     1681 aacctgtgtc aacttttatg ttacagcgat ggccgggtca ttcgggcagg gcggcgcacc
     1741 ttccggttgc ggctgcgaag gttatggcgg cggcggcgga tggtacggcg gtgcgggaag
     1801 cggaaactgc cgcggcggcg gtggcggcag cggttacatc atcgcgcttg cttccaacca
     1861 ggtggcaacg gcaggtgtcc gcgccggtaa cggaatggtc gtgatcagca caggacaatc
     1921 ttccggcgca acagttacac agctggcagg tcttgcttca ggttccactt tcccggttgg
     1981 tattacgaca aatacattcg ctgtttccga tgtgtttggt aatgccgaca cctgcagctt
     2041 tacagttacg gttaccgatg tggaaaatcc cgtgatcagc aatacgcctt cttccatcag
     2101 tgtgaataat gacgcaggac tttgcggagc cgtcgtttcc tggacgcctc ccacggcaac
     2161 cgacaattgc agcgcaacac ttacttccaa ttacaacagc ggcgatcttt tcccgccggg
     2221 aactaccacg gtaacttata ccgcaaccga tccttcggga aatacggcca cttccagttt
     2281 tacagtcaca gtcaccgata cggaagcgcc tgtgctttcc tgcccggctg atctttcgat
     2341 ctcaacagac tccggctcct gcgttgcaac cggcgtcata ctgggcaatg ccaatgcaac
     2401 agataattgc agcgtagcgg gcgtagtcaa tgatgcgccg tcttccttca tgcagggaac
     2461 aaccgtcgtt acgtggacgg caacggatgt gaacggaaat tccactacgt gtacacaaaa
     2521 cgtcacggtg accgatatgg aagcgccttc tctcgtttgt ccttccgata taacggtctc
     2581 tgtcgatccg ggttcctgcg atgcgacgaa tgtgctgctc ggcacagaga ctgcatcgga
     2641 caactgtgcg attgcgacgg tggtgaatga tgcgccgaat tctttcccgg caggaacaac
     2701 ttccgttaca tggaccgctt cggatgcagc cggcaattct accacctgca cacagctggt
     2761 caccgttgtc gacagtgaac cgccggtcat ctcgtgcact tccgacacga cgatctgttc
     2821 cggcgtatat gtgttcaatg caccggtggc ttccgacaat tgttccgcaa cagttacgca
     2881 aatagcaggt ccgaacagcg gcgattccct cgcggccgga aactatacgc tgtcattttc
     2941 cgccgtcgat ccttccggaa acatggcgat gtgcagctac caggtaacgg tgaacccttc
     3001 accttccgtt tctgtttcgt ccaacccgac gacgatctgc gttgacgacg ctccggctgc
     3061 gctttcagga acaccagcgg gaggaacatg gagcggcccg ggcgttaccg gaagcacgtt
     3121 cgatccgcag gctgcgggcg atggtgcgca cacgcttacg tactcggtca ccggcgcgaa
     3181 ttcctgcaca ggcagcgcat cgttcacgat caccgttgat ccctgcacgg gcataaaaga
     3241 aaatacgcag actctttttg cgatgacgcc gaaccccgcg gaccaggtgg tttcattcac
     3301 gctttccgga agcggaacgc tggagatcat ggatgcaacg ggcaagctcg tcctttcgaa
     3361 aaccatcagc ggcgacaggt atgaccaggg cgtcggtgat tttgcggatg gcgtttatac
     3421 cgtccgtttc acttcaaacg aagggaaaat acaggtggcg aagctgatga tcaaccactg
     3481 ataaaaaaac cgtcacacca ggcacctccg tctttccggg cgggggtgtt tttttttagg
     3541 tgcgcggctc attttgactt caccggataa gcggaagaca gaccggttaa attaacattt
     3601 gaatttctgc cctttttttc ccatcttcat tatcccaaaa aagacgaaca gtcaactcac
     3661 caaaaccttt cacatgaaaa agcgcttact tgctattttg atcgcgtgca ttccccttgc
     3721 cggatggtcg cagaatgttt tcaccgattt ccaggacgga aagatctggt tccaggtgaa
     3781 ggacgattac cggatcaacg cgccgctgca ggaagatccg catcatcttc cgatcggcac
     3841 gcttggtttc ctcaatggta tttcaaaaaa tcaccagctg acgaatcttt cacggccttt
     3901 ttacgccgca aaaaattcac ccattctcca gcggacattc ctcctcgaat tttccgatgc
     3961 ggccaacatc atgtccgtca tcagcgagct gaaagcaacc ggcaatgtcg tgtatgcgga
     4021 gcgcgttccg ctcgatcatg aatgtctcac gccgaacgat cct
//
LOCUS       DASTSF010000544         3613 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1247823,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000544 DASTSF010000000
VERSION     DASTSF010000544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3613
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1247823"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..436
                     /locus_tag="VFU15_15545"
     CDS             <1..436
                     /locus_tag="VFU15_15545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014085416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU4719258.1"
                     /translation="EYDPAEYNILLRPVLDGFADVVYGSRFMGGRPHRILFFWHTIGN
                     KFLTTLSNMFSNLNLSDMETCYKLFDAKMVKSLLLKENRFGFEPEVTAKISRIPNVRI
                     YEVGISYYGRTYEEGKKIGWKDGFRAIWCIIRYNLFDTRHLK"
     gene            441..2846
                     /locus_tag="VFU15_15550"
     CDS             441..2846
                     /locus_tag="VFU15_15550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719259.1"
                     /translation="MKFLNTLKRIFSDKGLLLTAGLGLTMLLYYYGGLLLHPSLTYFG
                     AGNPDGFQIYYETLYHVKFDHGFWRQTSINYPYGESIFYTGALPFINNFAKLFGHGAG
                     GFAVWLINVVSLFSVPAGAVFIYAIFRHLRVTWFYGALCSVGIAFLSPQIIRLTGQYS
                     LSWVFLVPGLFYLLLRFYDYPSLKKSFAIAFLTFLAITTHIYFFLFFVGIGLVYWFVL
                     FITRDRGFGRIVFALKHGSIQFILPLLIFQFIVMYSDTVTDRSQVPWGYLVYFSDLYG
                     VFYPFLSPYERLVHSFVSPDVNHLEWEGISYVGAAAIIGMVMVALVQLFMLFRHRFRF
                     RISERKGIFFRVLWWTKEIFRLLLDRLRLLVSVTDNKVLNIFFWTSVLLLYISFALPF
                     RDQHEDWLLYLGPVRQFRSIGRLAWVFYYALNIVVVYRLHKVFMQRKAGKIIFTALIP
                     GLMFYDGYYNNYRNFDLMNSRIPELEDNANTLPQDQWLTKIKTQDYQAILPLPFFHNG
                     SENIAIFPPDGAIVKETFVLSLKTGLPVMAVQSGRVSLGQTMKVVALEYDPVKEIPVL
                     DDLRDKRPLLVIVKPGSINENEKRILSLATKVGESTGYSVYSLDPAALRKLPGMAYDS
                     ARAEFDTVKKYPVGNFFCTDSMAHIIYDNYENGNGKPYRGNHSQGSDLHTFTVVVDKD
                     VNDTGKYIASFWVNDISKDCYGRSLLKLTTTDSTGKNVDIYSSFSVQLKAIDGPWGLI
                     EIPFTMPGPKGHFQIIFWNADVKPSSIIYTDELLVHSQNADVFRQDGESLYFNNRIYS
                     PAK"
     gene            2843..3571
                     /locus_tag="VFU15_15555"
     CDS             2843..3571
                     /locus_tag="VFU15_15555"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU4719260.1"
                     /translation="MKLSIILPSYKSGETLGRQLPPLKKWLEEKNISHEIIVVDDGSG
                     DNGETKQAAEENGCVYILHEVNRGKGAAVRTGMLVAKGDFRIFTDADIPFEYESFGLF
                     LHYLEDKEFDLAVGDRTLPESKYFSEIKGSRRAGSSIFTFFVGRFVTTGLFDTQCGLK
                     GFSAKAAEDLFSVARLDGFAFDVELLYIALKRNYDIKRLPVSFRSSHDHSSVSLMRHA
                     PGMIMDLFRIKWNQVTGRYRRTGK"
BASE COUNT          797 a          955 c          934 g          927 t
ORIGIN      
        1 cgaatacgat cccgccgaat acaacatcct gcttcgtccc gtgctcgacg gctttgcgga
       61 cgtagtgtac ggatcgcgct tcatgggcgg acgcccgcac cgcattcttt ttttctggca
      121 tacgatcggc aacaaattcc tcaccacgct ctcgaacatg ttctccaacc tgaacctttc
      181 ggatatggaa acctgctaca agctgttcga tgcgaaaatg gtgaagtcgc tcctgctgaa
      241 agaaaaccgt ttcggcttcg agccggaagt gacggccaag atatcgcgca tccccaatgt
      301 gcggatctac gaagtcggaa tttcctatta cgggcggacg tacgaggaag gaaaaaagat
      361 cggctggaaa gacggcttcc gcgccatctg gtgcatcata cgctacaatc ttttcgacac
      421 ccgccacctg aaataaggga atgaaattcc tcaacactct caagcggatt ttcagcgaca
      481 aagggctgct gctcaccgca ggcctgggat tgacgatgct gttatattat tatggcggat
      541 tactgctgca tccttcgctc acctatttcg gcgccggcaa tcccgacggg ttccagatct
      601 attacgaaac gctttaccat gtgaagttcg atcacggctt ctggcggcag acgagcatca
      661 attaccctta cggcgaaagt attttttaca ccggcgcgct gcctttcatc aacaactttg
      721 cgaagctctt cggccatggt gccggcggtt tcgccgtatg gctcatcaat gtcgtttcgc
      781 ttttttctgt tcccgcagga gccgttttca tctatgccat cttccgccac ctgcgcgtaa
      841 cctggtttta cggggcgctc tgctcggtgg gaattgcatt cctttctccg cagatcatcc
      901 gcctgacggg acagtattcg ctttcgtggg tgtttcttgt gcccggactt ttttacctgc
      961 tcctgcgttt ctacgattac ccttcgctga aaaaaagttt tgcgatcgcg ttcctgactt
     1021 ttctcgccat caccacgcac atctatttct ttcttttttt cgtcggcatc ggtcttgtgt
     1081 actggttcgt tctgttcatt acccgcgacc gcggcttcgg gcgcatcgta tttgcgctga
     1141 agcacggcag catccagttc attcttcccc tgctgatctt ccagttcatc gtcatgtatt
     1201 ccgatacggt taccgaccgc tcgcaggtgc cgtggggcta ccttgtttat ttttccgatc
     1261 tctacggtgt tttctatcct ttcctgagtc cgtacgaaag actggttcac agtttcgttt
     1321 cgccggatgt gaaccatctc gaatgggaag ggatttccta cgtcggtgct gcggcgatca
     1381 tcgggatggt gatggtggcg ctggtgcagc tgttcatgct gttccgtcac cgcttccgtt
     1441 tcaggataag tgaacggaaa ggaatttttt tccgggtgct ctggtggacg aaagaaatat
     1501 tccgcctgct cctcgatcgc ttacgccttc ttgtttccgt tacggacaat aaagtcctga
     1561 atattttttt ctggacgtcc gtgctgctgc tgtacatttc tttcgcgctc ccgttccgcg
     1621 atcagcatga agactggctg ctgtacctcg gacctgtgag gcagttcagg agcatcgggc
     1681 gcctggcctg ggttttttat tatgcgctga acatcgttgt cgtttaccgc ctgcacaaag
     1741 ttttcatgca gcggaaagcc gggaaaataa ttttcaccgc gcttattccc gggctgatgt
     1801 tctacgacgg gtattacaat aattaccgga attttgacct gatgaactcg cgtatcccgg
     1861 agctggagga taacgcgaac acacttccgc aggaccagtg gctgacgaaa ataaaaacgc
     1921 aggattacca ggcgatattg ccgctcccgt tctttcataa cggatcggag aacatcgcca
     1981 ttttccctcc cgacggagcg atcgtcaagg aaacattcgt cctgtcgctg aaaaccgggc
     2041 ttcccgtgat ggcggtgcag tcgggaagag tttcgctcgg gcagacaatg aaagtcgttg
     2101 cgctggaata cgacccggtg aaggagatcc cggtgcttga tgatctccgc gacaagcgac
     2161 cgcttcttgt catcgtgaaa ccgggcagca tcaatgagaa tgaaaagaga atactttcgc
     2221 tcgccacgaa agtgggcgaa tcgaccggct attccgtgta ttcgctggat cctgcagcat
     2281 tgcggaagct gcccggaatg gcgtacgaca gcgcacgtgc ggaattcgat accgtgaaga
     2341 aatatcccgt gggtaatttt ttctgcacgg actcgatggc gcacatcatt tacgataatt
     2401 acgaaaacgg caatggcaaa ccgtaccgcg gtaaccattc acagggaagc gacctgcata
     2461 cttttacggt tgtcgttgat aaggacgtga acgatacggg aaaatatatc gcctcgttct
     2521 gggtaaatga tatttcgaaa gattgctacg gccgcagcct gctcaagctc accacgaccg
     2581 acagcaccgg gaagaacgtg gatatttatt cttccttcag cgtgcagctg aaagcgatcg
     2641 acggaccttg gggacttatt gaaattccgt tcacgatgcc cggcccgaaa ggccattttc
     2701 agatcatctt ctggaacgcc gacgtaaaac cgtccagcat aatctataca gacgagttgc
     2761 tcgtgcattc gcagaacgcc gatgtgttcc ggcaggacgg agaatcgctg tattttaata
     2821 acaggatcta ttcgcccgct aaatgaagct gagcattata cttccttcct acaaaagcgg
     2881 ggaaacgctt ggccgccagc tgcctccgtt gaagaaatgg ctggaagaaa aaaatatttc
     2941 gcacgagatc attgtcgttg atgacggttc cggtgataat ggcgaaacga aacaagccgc
     3001 ggaagagaac ggctgcgtgt atatccttca cgaagtgaac cgcgggaaag gcgctgcggt
     3061 gcgcacggga atgctcgtcg cgaaaggcga cttccggatt ttcaccgacg cggatattcc
     3121 tttcgagtac gaatcgttcg gacttttcct gcattacctc gaggacaagg aattcgatct
     3181 cgccgtcggc gaccgcacgc tgccggagtc gaaatatttt tctgaaataa agggatcgcg
     3241 ccgcgcgggc agcagcatct tcacgttttt cgtgggacgt ttcgtaacga cgggattgtt
     3301 cgatacgcag tgcggcctca agggattcag cgcgaaagcg gcggaagacc ttttttccgt
     3361 ggcgcgcctc gacgggttcg cgttcgatgt ggagctgctg tacatcgcgc tcaagcggaa
     3421 ttacgatatc aagcgcctgc ccgtttcgtt ccgcagctcg cacgatcatt cgagcgtcag
     3481 cctcatgcgt catgcgcccg gcatgatcat ggatcttttc cgcatcaagt ggaaccaggt
     3541 aacgggccgt taccgcagaa cagggaaata agataatttt accgcgcggt tgatctgcgg
     3601 ataaaatgct tta
//
LOCUS       DASTSF010000545         2238 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_772560,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000545 DASTSF010000000
VERSION     DASTSF010000545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2238
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_772560"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..533
                     /locus_tag="VFU15_15560"
     CDS             <1..533
                     /locus_tag="VFU15_15560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019946275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEU4719261.1"
                     /translation="PMHSEIQFKVKHLVISTVTGTFTRFSADVETDGENFETAKIHFS
                     ADANSISTGMEQRDGHLKSADFFDAGNFPTIDFISDSVVKKGDAQFTVNGKLTMRGVT
                     KPVSLNVEFGGTMTDPYGNFKAGFEVTGSLKRKEFGLSWDAVTEAGGVVVSDEVKIVA
                     NVQFAKVAAEVEKAQA"
     gene            complement(538..>2238)
                     /locus_tag="VFU15_15565"
     CDS             complement(538..>2238)
                     /locus_tag="VFU15_15565"
                     /inference="COORDINATES: protein motif:HMM:NF040058.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719262.1"
                     /translation="TLLHPADTELIEGDGYFEGSDFITSVSERPVTVEQIVSVTFYET
                     WLYDSIGGTLVKLVHGMIPHILETDQQTGELRAKPLFYIPFSSASLSGDAVATLTGFT
                     YDMRTDDSLFDVSLPGSTMAFVNSADRAKEDAGLGKIISGATGSKKTSFFKPVYPYGV
                     PFGKKELKTLRPLLPASNALRFYEDWVFDAKRMVFMKMVHGVVLLQKKNAHMAEYDPS
                     PVTAHAFVSLNGFVPSPFAFPPLARVGRVEYASLFRNRPDFPDADFMLHPDSAAMTAM
                     ANSIWTSARTGKTPVYDQRNTYGTFIESVDGSFLAPPDSIFFVREEMVIIDSMGNETW
                     QDVSVALDSTLFSGFRFAESWTYDAAHRTLGKTIYSLSAMYVKTDYETDTPLGFYPLF
                     TVYPADTVKDPSTLMKPEFLVARNILSPVRINMNMANASPEYSTDYQVPCAVYSDDPT
                     CDIAPSSRYLFVQQIFNDVMAGKIAAYDSLGNMLSVQQLKTKVDALKDSTYAPGDLPS
                     EYMLFDELIFAEDWYFNPATSQLYKKVTAVTFTGKNPDASDGTGAAGRRTSVFTVKMN
                     "
BASE COUNT          530 a          565 c          640 g          503 t
ORIGIN      
        1 acccgatgca ttcggagatc caattcaaag taaagcacct ggtgatctcg acggttaccg
       61 gaacattcac cagattttcc gccgatgtgg aaaccgacgg cgagaatttc gaaacagcga
      121 agattcattt ttccgccgat gcgaacagca tcagcacggg gatggagcag cgcgacgggc
      181 acctgaaaag cgccgatttt tttgacgcag gcaattttcc caccatcgat ttcatttccg
      241 attcagtggt gaaaaaagga gatgcgcaat tcaccgtgaa cggcaaactc acgatgcgtg
      301 gcgttacgaa accggtttcg ctcaacgtgg aattcggcgg cacgatgacg gatccttacg
      361 gcaacttcaa ggcgggcttt gaggtgacag gttccctgaa gcggaaagaa ttcggtcttt
      421 catgggatgc agtgacggaa gccggcggtg tggtggtcag cgacgaagtg aagatcgtgg
      481 ccaatgtaca gttcgcgaag gtggcggccg aagtggagaa ggcccaagcc tgaacgctca
      541 gttcattttt acggtgaaga cggatgtcct ccgtccggca gccccggttc catcactggc
      601 atcggggttt tttcctgtaa aagtaaccgc ggttactttt ttgtacagct ggctggtggc
      661 cgggttgaaa taccagtctt ccgcgaaaat gagctcgtca aacaacatgt attccgacgg
      721 aagatcgccg ggagcataag tgctgtcttt cagcgcatcg accttcgttt tcagctgctg
      781 cacggaaagc atgttcccga gactgtcgta cgcggcgatc tttccggcca tcacatcgtt
      841 gaagatctgc tgcacgaaaa gatagcggga agaaggcgca atgtcgcacg tcggatcatc
      901 ggaataaacc gcgcagggga cctggtagtc ggtgctgtat tcgggtgatg cgttcgccat
      961 gttcatattg atccggaccg gcgacaggat gttccttgcg acgaggaatt ccggtttcat
     1021 caacgtcgaa ggatctttca cggtatcggc ggggtatacc gtgaacagcg ggtaaaaccc
     1081 gagaggtgtg tcggtttcgt aatccgtttt cacatacatg gccgaaagcg aataaatggt
     1141 ctttccgagt gtccggtgag cggcgtcgta cgtccagctt tccgcgaagc ggaacccgct
     1201 gaaaagagtg ctgtccagcg cgacggacac gtcttgccat gtttcgttgc ccatggaatc
     1261 gatgatcacc atttcctcgc gcacgaagaa gatcgaatcg ggcggagcca ggaaagatcc
     1321 gtcgacgctc tcgatgaatg tcccgtatgt gttgcgttga tcgtacacgg gtgttttccc
     1381 ggtgcgtgcg gaagtccaga tcgaattggc catcgccgtc atcgccgccg agtcgggatg
     1441 aagcatgaaa tccgcgtcag gaaaatccgg ccggttgcga aagagcgaag catattccac
     1501 ccggccgacc cgcgccagcg gcgggaaagc gaaaggagac ggaacgaagc cattcaggga
     1561 aacgaacgca tgcgccgtca ccggcgacgg atcgtattct gccatgtgcg catttttttt
     1621 ctgcagaagc acgaccccgt ggaccatttt catgaagacc attcttttcg catcgaacac
     1681 ccaatcttcg taaaaccgca gcgcattgga tgcgggcagc aatggacgaa gtgttttcag
     1741 ctcctttttc ccgaacggta ctccgtaagg ataaaccggt ttgaagaacg atgttttttt
     1801 cgacccggta gcgccggaga ttattttacc gagaccggca tcttcttttg cacggtcggc
     1861 ggaattcacg aacgccattg tgctgccggg aagcgaaacg tcgaaaagac tgtcatcggt
     1921 gcgcatgtcg tatgtaaaac cggtgagcgt tgcgacagca tcaccggaaa gagatgcgga
     1981 tgagaacggg atgtagaaaa gcggtttcgc cctgagctcc ccggtctgct gatctgtttc
     2041 gaggatatgc gggatcattc cgtgcaccag cttgaccagc gttccgccga tgctgtcata
     2101 gagccaggtt tcataaaacg tcacggaaac gatttgttca acggttacag gacgctcgga
     2161 tacgctggta ataaaatccg atccttcaaa atacccgtct ccctctatga gttccgtgtc
     2221 cgcaggatgg agaagtgt
//
LOCUS       DASTSF010000546        12880 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1666127,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000546 DASTSF010000000
VERSION     DASTSF010000546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 12880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12880
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1666127"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1736)
                     /locus_tag="VFU15_15570"
     CDS             complement(<1..1736)
                     /locus_tag="VFU15_15570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014680925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprB repeat-containing protein"
                     /protein_id="HEU4719263.1"
                     /translation="MFRTHKHFAGLLRLIVFFFAMTAGEELFSQAPGLYINEVSQGPS
                     GSKEWVELLVVGTPTCYSIPTLDLRGWYIDDNNGNHATGAGTGIAQGCVRFRNDPFWS
                     AIPIGTIIVIYNDADLDPAIPAQDLSMTDGNCRLVIPISNCTLFEKNTSTPSTATAIY
                     PTTGFSTCGSWTNISMANGDDSFQTIDPSGNLFHSVSWGNNTLNDIIYFTGPATGNVA
                     RMMNSSNNNISQQSNWVMVPVASENPGVANDAANQAWICSMNNGCTPPAPIALTTTQV
                     NASCTCTGSATVNATGGFTGCGSPYTYSWAPGGGNTATASGLCAGNYTVTVTDIIGCV
                     QTHTVNITASTAFTLSTTQTNVLCNGGSTGSASVTVTGGTGPFTYSWSPSGGSGANAT
                     GLSAGTYVVTVTDATLCSGTATVTITQPTPLSATTSQTNVSCNGGNNGSATVNASGGT
                     SPYSYSWSPSGGTGATASSLSAGTYTCTITDANSCTTTQSFTITQPTPLSATTSQTNV
                     SCSGGNNGSATVNGSGGTSPYSYSWSPSGGTNATASSLSAGTYTCTITDANSCTTTQS
                     FTITQPTPLSATT"
     gene            complement(2134..3072)
                     /locus_tag="VFU15_15575"
     CDS             complement(2134..3072)
                     /locus_tag="VFU15_15575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719264.1"
                     /translation="METDKMVHERFGPEAFMQKNKTAIRITVATALALSLAGNVFLQH
                     RNASLAGEKSILSEQIDDLTYAKLDAQKQYAEAKNQMDALRGQNATLDQLINQKTKEL
                     DDAKMKIDQLTLANASLPALKKQIAELKKINADFLARVDALNAQNAQLTAENTTLKEK
                     NSTLTVKVDDYSRKIDQGKELRAANIGVQSFRLKRNAKEIQTGKARKTDRITVDFDIL
                     ANTLADAGKRNVQVILRDPSGLVMTEDENGDNYLASTDAAAAQTKNLSVDYDRNDTHL
                     SVNYDYNNRMKLSKGVYTAEIYVDGKLAGKKEFSLK"
     gene            complement(3222..4178)
                     /locus_tag="VFU15_15580"
     CDS             complement(3222..4178)
                     /locus_tag="VFU15_15580"
                     /inference="COORDINATES: protein motif:HMM:NF024877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reductive dehalogenase domain-containing
                     protein"
                     /protein_id="HEU4719265.1"
                     /translation="MNERNTYSPPPFAWDEWYASVGGRTISIPEVDRLLKQIYEDQYA
                     RFDGPVAAKKKEFASPREAAAHIKQIARESGADITGICLIEPSDVYKGKTVTEKFAVA
                     VGQRMRWREFQVVPSEASAIECIRVYHSLGETVIKLAAYIRSLGYTCSIEHPIGDSNL
                     LHIPIGLKAGFGELGRHGSIIHPRLGPLFRMGSVITSIEMEIDHPIDAGIAKFCDNCK
                     ACRIYCPAKAIPDERSAEAGKDHLGNDRYVVDTGRCFPYFAKNNYCSICLPVCVYHHK
                     EWAKDFEGFQTKLFPEVIMHEPPAPADSVAAEKKHRYNKLGR"
     gene            complement(4213..4743)
                     /locus_tag="VFU15_15585"
     CDS             complement(4213..4743)
                     /locus_tag="VFU15_15585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719266.1"
                     /translation="MLNSTLSGVILAAVIGLLLLLLVMVSRRLNNVKPGWYILAFMIV
                     GIAVAVVYMSNTHKVYVVTGDDYSATGVNVKNYVLWGEDLDYAMKDGKTLHAKVRGSS
                     SAVINETFLPMIVVEQIYSNSSFGASPESYEVGRDSITVVPVSSIGYMPGESFPKSVS
                     ADAGTSSKSEWGLRIR"
     gene            complement(4951..6231)
                     /locus_tag="VFU15_15590"
     CDS             complement(4951..6231)
                     /locus_tag="VFU15_15590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009283119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU4719267.1"
                     /translation="MSFDKLNLITPILTALNEEGYTQPTPIQQQAIPIVLEGRDLLGC
                     AQTGTGKTAAFAIPILQLLYNSPAQGNARKLIRTLVLTPTRELALQIGESFSAYGRHT
                     RLKNLVIFGGVGQKPQTDALRNGVDILVATPGRLLDLMDQGYVSLQNIEYFVLDEADR
                     MLDMGFVHDVKRIIAKLPQKKQTLFFSATMPGEIVKLSSSILRNPVKVEVTPVSSTAE
                     TVQQLIYFVNKPDKKNLLIHVLKDTSITTTLVFTRTKHGADRVARDLTRAGIKTEAIH
                     GDKSQNARQRALGNFKAKKTRVLVATDIAARGIDVDELTHVINYEIPNVPETYVHRIG
                     RTGRAGASGIAISFCDGEERAYLRDITKLIGKQIPSVEEHPFKNAGAAPVHSETVQHV
                     RSNGHARSGAGHQKRSAENAPRRRKKWFGRRKTA"
     gene            complement(6539..6739)
                     /gene="xseB"
                     /locus_tag="VFU15_15595"
     CDS             complement(6539..6739)
                     /gene="xseB"
                     /locus_tag="VFU15_15595"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008238604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEU4719268.1"
                     /translation="MKDISYTDALNELQEISRAIENESIPIDELAVKVKRASELIALC
                     RAKLRATEEEVKKIIGEMGETE"
     gene            complement(6811..8166)
                     /gene="xseA"
                     /locus_tag="VFU15_15600"
     CDS             complement(6811..8166)
                     /gene="xseA"
                     /locus_tag="VFU15_15600"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEU4719269.1"
                     /translation="MQPGSQLRLSEFCSLISDTLKKTFSDRTWWVLAEIKERNDKGEH
                     IYFELVEKSENGKDIIARMNGNVWRREAVAAFRNFEDITGKRPERGMQVLVKAGVNYH
                     PLYGLSLQLFDIDPQHMLGQLEMQRRKTISLLASKPGIRLADGVFSTPNKKLQLPVVI
                     RRIAIITSASAAGYHDFTHTLTENIFRYKFECTPFFSILQGENAAEQMKLQLLNIYHE
                     TQKGKTFDAVVMIRGGGSQSDLLPFDDFNLAHAVARFPVPVITGIGHLKDESIVDMVA
                     NSTTKTPTQAAEFIIDHNRAFEEEIEGLRELLSLRVHNQLTKHHRRLDHTGAVLNNGT
                     LQLLHTAENRIQRHREKIGTASLRLLSSASQRMGELQRNFPPLTRRLIEKQIHAMDLF
                     SERVKNLHPENILKRGFAIVKKKEAYVVSAKQVKKEDAVEILLRDGMLEVKVDNVKHE
                     T"
     gene            complement(8230..8895)
                     /locus_tag="VFU15_15605"
     CDS             complement(8230..8895)
                     /locus_tag="VFU15_15605"
                     /inference="COORDINATES: protein motif:HMM:NF016155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid biosynthesis protein"
                     /protein_id="HEU4719270.1"
                     /translation="MKRNELISRYERPAKFFAVALALLWAAGAIFFNAVSCLLLPVFF
                     LLECGLFFFFIPEPGKNLLRLALLVFAGIAGLEIINAQSEFIFGEITFGSTAWVTPGG
                     IPVLVLLLWTLAVLLVYDIAGKFTEDPFLRSALSAALLVALDFIAEGQAFRLDLWHWN
                     YVSPTVRNYLAWFFATFFFAYAGSVLKVEIKNPLSRTIFLALLIFFALPVLYFRLFPA
                     FAP"
     gene            complement(8892..9794)
                     /locus_tag="VFU15_15610"
     CDS             complement(8892..9794)
                     /locus_tag="VFU15_15610"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEU4719271.1"
                     /translation="MKSRSFLHWIIFLALALTWGSSFILMKRGLDVFTGFQVGALRIS
                     IAFLFLLPLAVKHLKPGVRKHWKAGAAMGLCGNLFPAFLFAKAETMISSSLTGMMNSL
                     TPLFTMVVGLFVFRSKVGKMQVAGVVIGLAGAIGLLQLGEQETTHSNALTGTGMVLLA
                     TLFYGISVNIIKAKLAHLNAVAITVLALCMTGPFAIAYLFTTDFFTRISTVPGAAESI
                     AYIAVLAILGTSLSVMVYNILIKEAGTLFAASVTYLIPVVALGWGMLDGEKVGWLHGA
                     LILVILAGVWMINKKQVARITEVQ"
     gene            9862..10080
                     /locus_tag="VFU15_15615"
     CDS             9862..10080
                     /locus_tag="VFU15_15615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEU4719272.1"
                     /translation="MAEKKITLRIAGMTCGHCAKTVSSAITELDGVKEADVDPATGMA
                     EVVFDDEKVSEAQIRKAVNETEVYSVPE"
     gene            10190..11623
                     /locus_tag="VFU15_15620"
     CDS             10190..11623
                     /locus_tag="VFU15_15620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005368840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEU4719273.1"
                     /translation="MEDDTEKPQRYPIYVGGEFVETATVLDVRNPYTGKVFAKTFLAG
                     EEELGKAITAAQKAQRVMAELPSFRKYKILRSISDTIFAERGRLAHELCRESGKPMRY
                     ALGEIDRAAQTFLIAAEECKRLPKEYISLDWTPAGEKKEGVVKYFPCGIVAGIAPFNF
                     PLNLAVHKIAPAIAAGCPIILKPASSTPVSTLGLAKIIAKTDLPKGAVSILPMDRETG
                     NRLVTDERFSLLSFTGSPEVGWKMKKNAGKKKVVLELGGNAGVIISQSADLENALKKS
                     LAGGFAYSGQVCIHAQRFFVQRSVFAPFIQRFVEMVSELKTGDPEELTTDISVMIDEE
                     NAKRVEQWVNEAVKEGAEILCGGNREGSFYRPTVLTETKSRMKVCAEEIFGPVVTVEP
                     FDSFDEAIAMINHTEFGLQAGVFTNDISEMDRAFSEIHCGGIILNDVPTFRVDHMPYG
                     GVKDSGLGREGVKYAIMDMMEARILVK"
     gene            complement(11687..12028)
                     /locus_tag="VFU15_15625"
     CDS             complement(11687..12028)
                     /locus_tag="VFU15_15625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010366645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU4719274.1"
                     /translation="MKPVSKNDAPHYLWGNDCDGWRLLDSPSLSVIHERMPAGTREKK
                     HFHEKAQQFFFVLEGEAVFYFENEKVSVKENSGIHIPAGKIHLIANETEEAIEFLVIS
                     EPPTKGDREEC"
     gene            complement(12036..12566)
                     /locus_tag="VFU15_15630"
     CDS             complement(12036..12566)
                     /locus_tag="VFU15_15630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719275.1"
                     /translation="MGSYIGIKIGVLVVLLVIRLAVRAGRSGKSSSSSFTPPQSRPSG
                     GSPFGTPSSGTNSFGNSGSGTFSSSGTGSLENSGSNPNPFSTPAVSNPFNTSGPRNYY
                     CKYCGKKFASVADLSADTCYKNPDPSGSQKHALYMGAEKKEYSCMRCGKKFPTLVLLS
                     SDYCMSNANGRHEAAL"
BASE COUNT         2819 a         3270 c         3665 g         3126 t
ORIGIN      
        1 gtcgttgcag aaagcggagt tggctgcgtg atggtgaaac tttgcgtggt ggtgcagctg
       61 ttcgcatcgg tgatggtgca ggtgtaagtt cccgcggaaa gcgatgaagc tgttgcgttg
      121 gttccgccgc ttggtgacca cgaataactg tatggacttg ttcctccgga tccgttgact
      181 gttgccgaac cgttgtttcc tccgctgcac gatacgttcg tttgcgaagt cgttgcagaa
      241 agcggagttg gctgcgtgat ggtgaaactt tgcgtggtgg tgcagctgtt cgcatcggtg
      301 atggtgcacg tgtaggttcc tgcggaaagc gatgaagctg ttgcgcctgt tccgccgctc
      361 ggtgaccacg aataactgta tggacttgtt ccgcctgatg cgttgactgt tgccgaacca
      421 ttatttcccc cgttgcagga aacgttagtc tgcgaagtcg ttgcagaaag cggcgttggt
      481 tgcgtgatgg taaccgttgc cgttccggaa cataatgttg catccgtaac ggttactaca
      541 taagtgccgg cggaaagccc ggtcgcgttt gcgcccgatc ctccgctggg cgaccacgag
      601 taagtgaatg gcccggttcc gccggttaca gtgacggaag cagaacccgt gctgccgcca
      661 ttgcaaagaa cattggtctg cgtggtggaa agcgtgaatg cggtggacgc cgtaatgttc
      721 acggtatgtg tttgcacgca gccgataata tcggtgactg tcaccgtata atttcctgcg
      781 cataatccgc tggcggttgc cgtgtttccg ccgccgggcg cccagctgta ggtataagga
      841 cttccgcacc cggtgaatcc cccggtcgcg ttgacggttg cggaaccggt gcaggtgcac
      901 gacgcattca cctgtgttgt ggtaagcgcg atcggcgcgg gaggtgtgca gccattgttc
      961 atgctgcaga tccatgcctg gttggcggca tcattggcga cgccgggatt ttcactcgcg
     1021 accggaacca tcacccagtt cgactgctgg ctgatgttgt tgttcgagct gttcatcatc
     1081 cgcgccacgt tgccggtagc cggtcccgtg aaataaataa tgtcgttgag cgtgttgttt
     1141 ccccagctga cggagtgaaa aagatttccc gacggatcga tcgtctggaa agagtcgtcg
     1201 ccgttcgcca tggaaatatt tgtccagctg ccgcacgtgg aaaagccggt ggtgggatag
     1261 atcgccgttg ccgtgctggg cgtcgatgtg tttttttcga agagcgtgca gttgctgatg
     1321 ggaatgacca gcctgcagtt tccgtcggtc atgctgaggt cctgcgccgg aatagccggg
     1381 tcgaggtcgg cgtcattata gatgacgatg atcgtcccga tgggaatggc ggaccagaac
     1441 ggatcattcc tgaagcgcac gcatccctgc gcaatcccgg ttcccgcgcc cgttgcatgg
     1501 ttcccgttat tgtcgtcgat gtaccagccg cgaagatcga gcgtgggaat ggaatagcac
     1561 gtgggcgttc ccacgacgag cagctccacc cattctttcg aaccactcgg tccctgtgaa
     1621 acttcgttga tgtaaagtcc gggcgcctgt gaaaaaagtt cttcacccgc ggtcatcgcg
     1681 aagaagaaaa caatcaaccg taaaagtcct gcgaaatgtt tgtgtgtgcg aaacatattc
     1741 aaggcattta atggaaccca atgcgtccgc aaggacactt tcagttttcc atcagcctga
     1801 aatggtttcg gcatatcccg tctgacgggg acgcttgaag gatattcact cctgctgaag
     1861 tggaacaaat atagacctaa cctgtttgct ttagcttatt ccggttttat aataatgtaa
     1921 acaaccggtc gtcgggaaaa aagaaatatt ggaaggtcat aaaccgggtt ccggccggaa
     1981 ttttttaaag atacggtctt gcagggagag ctcattcccc ggcgatgtta caattttcta
     2041 atacgtcctg ttgcggaaat aaatggaaaa gaaaagcacc acgttggtcg tgatgctttc
     2101 tgtccggaag atggggagac gcttctttcc gttttatttc aggctgaact cttttttccc
     2161 ggccagcttg ccgtcgacgt agatctcagc ggtatacacg ccctttgaaa gtttcatgcg
     2221 gttgttgtaa tcgtaattga ccgaaaggtg cgtgtcgttc ctgtcgtaat cgacagacag
     2281 gttcttcgtc tgcgccgcgg ctgcatccgt ggacgcgaga taattgtcgc cgttctcatc
     2341 ttccgtcatc acgaggccgg aaggatcgcg gaggatgacc tgcacgtttc tctttcctgc
     2401 atccgccagc gtgttggcga gaatgtcgaa gtcgacagtg atgcggtcgg tcttgcgcgc
     2461 tttgccggtc tgaatttctt ttgcattccg tttgagcctg aacgactgca cgccgatgtt
     2521 cgctgcgcga agctcttttc cctggtcgat cttcctgctg taatcatcga ccttcacggt
     2581 gagcgtgctg ttcttttctt tcagcgtcgt gttctcggcg gtaagctgcg cgttctgtgc
     2641 gttgagcgca tcgacccggg cgaggaagtc ggcattgatc ttcttcagct ccgcgatctg
     2701 ctttttcagc gcgggtaaag atgcgttggc cagcgtgagc tgatcgatct tcattttcgc
     2761 atcatccagc tctttcgttt tctggttgat cagctggtcg agcgttgcat tttgtccgcg
     2821 caaagcatcc atctggttct ttgcttcggc atattgtttc tgtgcgtcga gcttcgcgta
     2881 ggtaaggtcg tcgatctgtt cggaaagaat gctcttttcc ccggcgagcg atgcattccg
     2941 gtgctgaagg aagacgttgc ctgcgagaga aagtgcgagg gcggtggcga cagtgatcct
     3001 gatcgcggtt ttgtttttct gcataaaggc ttcggggccg aaacgttcat gcaccatttt
     3061 gtcggtttcc atgttggctg gtttatggaa gtataacgat gcatgccctg ccttttgtta
     3121 ttagcaatgg gttagctttt gctgaaaaga aattagaaac gttaaaagga tacaaaaaga
     3181 gagcgggaaa acacgcaaat gaaaaaaact gcaaaaaaag ctcagcggcc gagcttgttg
     3241 tacctgtgtt ttttttctgc agcgacagaa tccgccggcg ccggcggttc atgcatgatc
     3301 acttccggga acagcttcgt ctgaaaacct tcgaagtcct tcgcccattc tttgtgatga
     3361 taaacgcaca cgggaagaca gatcgagcag taattgtttt ttgcgaaata cggaaagcat
     3421 cttccggtgt cgacaacgta acggtcgttg ccgaggtgat ctttcccagc ttccgcgctt
     3481 cgttcatccg gtatcgcttt cgcgggacaa tagatgcggc acgctttaca gttatcacag
     3541 aatttcgcga tccccgcatc gatcgggtga tcgatctcca tttcgatcga cgtgatcacg
     3601 cttcccattc ggaagagcgg acccagtcta ggatgaatga tcgaaccgtg gcggcccagt
     3661 tcaccaaaac ctgccttgag gccgatcggg atgtgcagca ggttgctgtc gccgatcggg
     3721 tgttcgatgc tgcaggtgta gccgagcgaa cggatgtaag cggcgagctt tattactgtt
     3781 tcgccaagcg aatgatagac gcgtatgcac tcgatcgcgg aagcttcgga cggaaccacc
     3841 tggaattcgc gccagcgcat gcgctgacca acggcgacgg caaatttttc cgtcactgtt
     3901 ttccctttgt aaacatcgga cggttcgatg agacagatgc ccgtgatgtc ggccccggat
     3961 tcacgcgcta tctgtttgat gtgcgcggct gcttctcgcg gcgacgcaaa ttcttttttc
     4021 ttcgcagcga ccggcccgtc gaaacgcgca tactggtctt cgtagatctg tttcagcaaa
     4081 cggtccactt ccgggatgct gatcgtcctt ccgccgaccg acgcatacca ttcgtcccag
     4141 gcaaaaggcg gtggagaata tgtgttgcgt tcattcatgc ggcaggttcg gaaaataatg
     4201 gttgcaacga ttttaccgga tgcgtaatcc ccattctgat ttcgagctgg ttcctgcatc
     4261 tgctgaaacg cttttgggaa aactctctcc cggcatgtag ccgatgctgc tgaccgggac
     4321 gaccgtgatc gaatcgcgtc ccacctcgta cgattcggga gaagcgccga aagaagagtt
     4381 ggaatagatc tgttccacga cgatcatcgg taaaaaagtt tcgttgatca ccgcgctcga
     4441 agatccgcgc actttcgcat gaagcgtctt gccgtctttc atcgcgtaat caagatcttc
     4501 tccccagagc acataatttt tcacattgac gccggtggcg ctgtaatcgt cgccggtaac
     4561 gacataaact ttgtgcgtgt tgctcatgta cacgactgcg accgcaatac caacgatcat
     4621 gaacgcgagt atgtaccagc ccggttttac attattgagc cggcgggaaa ccattacgag
     4681 cagcagcagg aggagaccga tgacggcggc gagaatcaca ccgctgaggg ttgagttcag
     4741 catggaaagg gttttgacag gcgtgttgtt tcaaatatcg gataaatgtt cgagttcatg
     4801 tatttcctgc aacggtttgc agaccgatga tgcgtgccgt tttgcaggat gatcgctgcc
     4861 ggggaatgaa gctgcggcac gaacaaaaaa aattgccggg agcggactcc cggcaatcga
     4921 ttctgcaaaa gatggttttg tttttagcgc ttacgcggtt ttgcggcggc cgaaccattt
     4981 cttccggcgg cgcggtgcgt tttcggcaga acgtttctgg tgacctgcac ctgatcgtgc
     5041 gtgcccgttg gaacgtacgt gctgcaccgt ttcgctgtga acaggagcag ccccggcatt
     5101 tttgaacggg tgctcttcca ctgatgggat ctgtttcccg atcagcttcg tgatgtcgcg
     5161 caggtaagcg cgttcttccc cgtcgcagaa cgagatcgcg atgccgctcg ccccggcgcg
     5221 tcctgtgcga ccgatgcggt gcacatacgt ttcaggtacg ttcgggattt catagttgat
     5281 cacatgtgtc agttcgtcca cgtcgatgcc gcgcgcggcg atatcggttg ccacgagcac
     5341 gcgtgttttt ttcgctttga aattcccgag cgcgcgctgt ctcgcattct gcgatttgtc
     5401 gccgtggatc gcttcggttt tgatacctgc gcgcgtgagg tcgcgcgcta cgcggtcggc
     5461 tccgtgcttc gtgcgcgtga acaccagcgt ggtggtgatc gacgtgtctt tcagcacatg
     5521 gatgagcagg ttcttcttat cgggcttgtt cacgaaatag atgagctgct gaacggtttc
     5581 cgcggtggaa gaaacaggtg ttacttccac tttcaccgga ttgcggagga tgctggagga
     5641 aagcttcacg atctcgccgg gcattgttgc cgagaagaac agtgtctgct ttttctgcgg
     5701 cagcttcgcg atgatgcgct tcacatcgtg cacgaatccc atgtcgagca tgcggtcggc
     5761 ttcgtcgaga acgaaatatt cgatgttctg aagggaaacg tatccctggt ccataagatc
     5821 gaggagacgt cccggtgttg caacgagaat gtccacaccg ttgcgcaggg catcggtctg
     5881 cggtttctgc ccgacgccgc cgaagatgac gagatttttc aggcgcgtgt gacgtccgta
     5941 agcggaaaag ctttcaccga tctgcagcgc gagctcgcgt gtcggtgtga gcaccagcgt
     6001 gcggatgagt tttcgcgcgt tgccctgggc aggactgttg tacagcaact gcaggatggg
     6061 gatggcgaat gcggccgttt tcccggttcc ggtttgcgcg cagccgagca ggtcgcgccc
     6121 ttcgagtacg atggggatcg cttgttgctg gataggcgtg ggttgcgtgt agccctcttc
     6181 gtttaacgcc gtgaggatgg gcgtaatgag atttagtttg tcaaatgaca tgttgaatga
     6241 tgaattaata aactgcacgt gagagatcat cgatgtgtaa ccatcgtatc cctatgactt
     6301 tcttcactta tgcaatgact ggaaaattaa ctgaacgaat ttcagctgaa ggagggataa
     6361 ctttcgggac aaaggtaagc aggaatagtg tcgccccgac gcgggcagtt aaaaagtttg
     6421 aaagctgcag gatacggacc cgaaccacaa cacccgatct cccaatgccg gatcgcaaac
     6481 ggcaaactga aaaacaggcc ggaaaaactc ctcagatact gaactttcca actaatggtc
     6541 actccgtctc tcccatttcc ccgatgatct ttttcacttc ttcttccgtc gcgcgcagct
     6601 tcgcccggca aagcgcgatc agctcggagg cgcgtttcac ttttaccgcc agctcgtcga
     6661 tgggaatgct ttcgttctcg atggcgcgcg aaatttcctg gagctcgttc agcgcgtcgg
     6721 tatacgatat gtctttcatt ttactcattc aaaattttct gttcagcgtt ttcgcccgga
     6781 aaccggctca ttcgatttaa tatgactttc tcatgtctca tgcttcacgt tgtccacctt
     6841 cacctccagc atcccgtccc gcaaaagtat ttcaaccgcg tcttcttttt taacctgttt
     6901 tgccgacacg acatacgctt cctttttttt cacgatggca aagccgcgtt tcagaatatt
     6961 ttccgggtga aggttcttca cgcgttcgga aaaaagatcc atggcatgga tttgtttttc
     7021 gatcagcctt cgcgtcagcg gcgggaagtt gcgctgcagc tctcccatgc gctgcgaagc
     7081 cgacgacagc agccggaggg aagcggtccc gatcttttca cggtggcgct ggatacggtt
     7141 ctccgccgtg tgcagaagct gcagtgtccc gttgttcagc acggcgcccg tatgatcgag
     7201 acggcggtgg tgtttcgtga gctggttgtg cacgcgcaat gaaagcagct cgcgcaatcc
     7261 ttcgatctct tcctcgaacg cacggttatg gtcgatgatg aattccgctg cctgcgtggg
     7321 cgttttcgtg gtggaattgg cgaccatgtc cacgatgctt tcgtctttca ggtggccgat
     7381 gccggtgatg acaggaaccg ggaagcgcgc cactgcatgc gcaagattga aatcatcgaa
     7441 aggaagaaga tcgctctgcg agcctccgcc gcggatcatt acaacggcat cgaatgtttt
     7501 tcctttctgc gtttcgtggt agatattgag cagctgcagc ttcatctgct ccgctgcatt
     7561 ttctccctgc aggatggaga agaaaggcgt gcattcgaat ttgtagcgga aaatattttc
     7621 cgtcagcgtg tgcgtgaaat catgatagcc ggctgcggag gcggaagtga tgatcgctat
     7681 gcgccggatg acgacgggaa gctgaagttt tttattcggc gtggaaaaaa cgccgtcggc
     7741 gagacgaatg ccgggttttg aggcgagcag gctgatggtt tttcgccgct gcatttccag
     7801 ctgtcccagc atgtgctgcg gatcgatgtc gaacagctgc agggaaagtc cgtaaagcgg
     7861 gtggtagttc acgccggctt tcacgagcac ctgcatgccg cgttcgggcc gcttgccggt
     7921 gatgtcctcg aaattgcgga aggccgccac ggcttcacga cgccacacgt ttccgttcat
     7981 gcgcgcgatg atgtctttcc cgttctccga tttttccacg agttcgaaat aaatgtgctc
     8041 gcctttgtcg ttgcgttctt ttatttccgc cagcacccac cacgtccggt cggaaaatgt
     8101 tttcttcagc gtgtcgctga tgagcgagca gaattcggaa agtcggagct ggctgccggg
     8161 ctgcatgctt caaatgtagg aacggaacgc agaaattgcc ggtgaaaagc ggtttattct
     8221 gtcaacaatc tacggcgcga atgccggaaa aagacggaaa tacagcacag ggagggcgaa
     8281 gaaaataaga agcgccagga agatcgtacg cgaaagcggg tttttgatct ccacttttaa
     8341 aacgctgcct gcgtaagcaa agaagaaagt ggcgaagaac catgcgaggt aattccgcac
     8401 ggtgggcgaa acataattcc agtgccagag atcgagacgg aaagcctgtc cttccgcgat
     8461 aaaatcgagc gccacgagca gcgctgctga cagcgcggag cgcaggaacg gatcttcggt
     8521 gaatttcccg gcgatgtcgt acaccagcag caccgcgagc gtccagagaa ggagaacgag
     8581 cacgggaata ccgccgggcg tcacccacgc ggttgatccg aacgtgatct cgccgaagat
     8641 aaattcgctc tgcgcattga tgatctcgag tcccgcgatg cctgcgaaaa cgagcagcgc
     8701 gagacgcagc aggtttttgc cgggttccgg aatgaaaaag aaaaacagcc cgcattcgag
     8761 aaggaagaat accggcagca gtaaacagga aacagcgttg aagaagatcg ctccggccgc
     8821 ccacagcagg gccagtgcga ccgcaaaaaa tttcgccggg cgctcataac gcgaaatgag
     8881 ctcgttcctt ttcattgcac ttcagtgatc cgtgccacct gttttttgtt gatcatccac
     8941 acgcctgcaa ggatgaccag gataagcgca ccgtgcagcc agccgacttt ttcgccgtcg
     9001 agcattcccc atcccagcgc gacgaccgga atgagatacg tcaccgatgc tgcgaaaagt
     9061 gtgcccgctt ccttgatgag aatgttatac accatcacgc tgagcgatgt gccgagtatg
     9121 gcgagcacgg cgatgtaggc aatgctttcc gctgcgcctg gaacggtgga aatacgtgtg
     9181 aagaaatctg tcgtgaaaag atacgcgatg gcgaaaggtc ctgtcatgca caatgccagc
     9241 acggtgatgg cgaccgcatt caggtgcgcc agcttcgctt tgatgatgtt cacgctgatg
     9301 ccgtagaata acgtcgcgag cagcaccatt cccgttccgg tgagcgcgtt gctgtgtgtt
     9361 gtttcctgct cgcccagctg cagcagcccg atcgcgccgg ccagcccgat caccacgccg
     9421 gccacctgca tctttcccac tttgctgcgg aaaacgaaaa gcccgacaac catcgtgaac
     9481 aaaggcgtca gcgaattcat catgccggta agcgagctgc tgatcatcgt ttccgctttt
     9541 gcaaaaagaa acgccgggaa aagatttccg cacaggccca tcgcagcgcc ggctttccag
     9601 tgtttccgca caccgggttt caggtgcttc accgccagcg ggagaagaaa aaggaaagcg
     9661 atcgaaatgc gcagcgcgcc cacctggaaa ccggtgaaca cgtcgagccc gcgcttcatg
     9721 aggatgaaag agcttcccca cgtcagcgcc agcgcgagaa aaatgatcca gtgaaggaag
     9781 ctcctgcttt tcatgcggca aatataggcg gggaaaccat cgccattgct tcgttttcat
     9841 gctatgttgt aattttaacg tatggcagaa aaaaagatca cgctcaggat agcaggcatg
     9901 acctgcggtc attgcgcaaa gacggtttcg tcggcaataa cggagctcga cggggtgaag
     9961 gaggcggatg ttgatccggc aacaggcatg gctgaagttg ttttcgacga cgaaaaagtt
    10021 tccgaagcgc agatccgtaa agcggtgaac gaaacggaag tttattccgt gccggaatga
    10081 aacggctggc ggccctgttc ctgcgggagg atcggtgcta ccgctatccc gatacagctt
    10141 actgttgcga tctttcttta tcccgtttcg tgaaaatttt aaagaagata tggaagacga
    10201 tactgaaaaa ccacagcgtt atcccattta cgtgggcggc gagttcgtag aaactgcaac
    10261 ggttctcgat gtgagaaacc cgtacacggg aaaagtgttc gcgaaaacgt tcctcgccgg
    10321 tgaggaagag ctcggaaaag cgatcactgc cgcgcagaaa gcacagcgcg tgatggcgga
    10381 acttccttcc ttccgtaaat acaaaattct ccggagcatt tccgacacga tttttgccga
    10441 acgcggtcgc ctggcgcacg agctctgccg tgaatcggga aagccgatga gatatgcgct
    10501 cggcgagatc gaccgtgcgg cgcagacgtt ccttattgcg gcggaagaat gcaagcggct
    10561 gccgaaggaa tatatttccc tcgactggac gccggcaggt gaaaagaaag aaggtgtcgt
    10621 gaaatatttt ccctgcggga tcgtcgccgg gatcgcaccg ttcaattttc cgctgaacct
    10681 tgcggtgcac aagatcgcgc cagctattgc tgcaggctgc ccgatcattc tcaagcctgc
    10741 gtcttccacg cctgtttcca cgctcggact ggcgaagatc atcgcgaaaa cggacctgcc
    10801 caaaggcgct gtttccattt taccgatgga ccgcgaaaca ggaaacaggc tggtcacgga
    10861 cgaacgtttt tcgctgctgt catttacagg ttcgccggaa gtgggctgga aaatgaaaaa
    10921 gaatgccggg aagaaaaagg tcgtgctcga gctgggcgga aatgcaggcg tgatcatttc
    10981 acaaagcgca gacctggaaa acgcgctgaa gaaatcactt gccggcgggt tcgcgtattc
    11041 gggccaggtg tgtattcacg cgcagcgctt ttttgtgcag cggtctgtgt tcgcgccgtt
    11101 cattcagcgt ttcgtcgaaa tggtgagcga attgaaaaca ggcgacccgg aagagctcac
    11161 gaccgacatc agcgtgatga tcgacgaaga aaatgcgaag cgcgtggagc aatgggtgaa
    11221 cgaagcggtg aaagagggcg cggaaattct ctgcggcgga aaccgtgaag gaagttttta
    11281 ccggccgaca gttttgacgg aaacgaaaag ccggatgaaa gtctgcgcgg aggaaatttt
    11341 cggaccggtc gtcaccgtcg aacctttcga ttcgttcgat gaggcgatcg cgatgatcaa
    11401 ccatacggaa ttcgggttgc aggcgggtgt ttttaccaat gacatttccg aaatggacag
    11461 agccttcagc gaaattcatt gcgggggaat tatcctgaat gacgttccca cgttccgcgt
    11521 cgatcacatg ccgtacggcg gtgtgaaaga ttcgggtctc ggccgcgaag gcgtgaaata
    11581 cgcgatcatg gacatgatgg aggcgaggat actcgtcaaa tagatctggt aacctgtttg
    11641 aagagttgga aagctgaaag gttgggaagt ttagcgagcg caggattcag cattcctccc
    11701 ggtcgccttt cgtcggcggc tccgagatca cgagaaattc gatcgcttct tccgtctcgt
    11761 tcgcgatcaa atggattttc ccggcgggaa tgtgaatgcc ggaattttct ttcacggata
    11821 ctttttcatt ttcaaaataa aaaaccgctt ctccttcgag gacgaaaaag aactgctgcg
    11881 ctttttcgtg gaaatgtttt ttctcgcgcg tacccgcagg catgcgttcg tggatcacgc
    11941 tcagcgacgg agaatcgagc aggcgccagc cgtcgcaatc gtttccccat aaataatggg
    12001 gtgcatcgtt tttgctgact ggtttcaccg gaagatcaga gggcggcttc gtgccttccg
    12061 ttcgcattgc tcatgcagta atcggaggaa agcaggacga gcgtggggaa tttttttccg
    12121 caacgcatgc aggaatactc ttttttttcc gctcccatgt acaacgcatg cttttgcgaa
    12181 ccggacggat cgggattttt gtagcaggta tctgcggaaa gatcggccac cgatgcgaat
    12241 ttcttcccgc agtatttgca gtaataattc cgcggaccgc tcgtgttgaa cgggttggag
    12301 accgccggtg tgctgaacgg gttcgggttt gatccggaat tttccagcga cccggttccc
    12361 gatgagctga atgtgcccga tccgctgttg ccgaatgaat tggttcccga ggaaggcgtg
    12421 ccgaacgggc ttccgcccga aggacgcgat tggggcggtg tgaatgacga tgaagatgat
    12481 ttgccgctgc gtccggcgcg cacggccagg cggatgacga gcagcacgac aagaactccg
    12541 attttaattc cgatatagct tcccattggg cgaggtttaa gtggtgaaat gaggtcagac
    12601 gaatctacaa attagtttaa tgctttgcgt accgttttac aggaaaccgg cccggcaggt
    12661 tcccggaatt gttaccgccg gatcgaaaaa acagcagtga acggcagaca cccagcgcgg
    12721 aacttcattc caccttctgc ttccggatca cgcgcgcgaa ccatttcggg aaatgacgtt
    12781 tcagccacac ggccttgatc tcgcgtcctc cgataaggat ctcctctttg ttctttttca
    12841 tggcatcgag gatgatggtt gcgcattcgt ccgcaggcat
//
LOCUS       DASTSF010000547         2757 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1431463,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000547 DASTSF010000000
VERSION     DASTSF010000547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2757
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1431463"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2413
                     /locus_tag="VFU15_15635"
     CDS             <1..2413
                     /locus_tag="VFU15_15635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018478181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEU4719276.1"
                     /translation="TPEYGAASQLEKIDMLDFADLIALNKFDKRGALDALRDVRKQYK
                     RNHQLWQTDDDKLPVVGTIASQFNDPGMNHLYRMLIDKIAEKTGADLKSHFEITKEMS
                     EKIYIIPPARTRYLSEISENNRGYDKKVKQQADVAQKLYALRKAIDVMSANKAAVASL
                     EEEYKRLLLELDPKNLKLIKEWPEKAQQYKNEFYVFKVRDKELKIKTHYESLSHTLVP
                     KIATPRYEAWGDLLQWLAQENVPGEFPYTSGIYPFKREGEDPTRMFAGEGGPERTNKR
                     FHYVSLGMPAKRLSTAFDSVTLYGRDPDYRPDIYGKIGNSGVSICCLDDAKKLYSGFD
                     LADPKTSVSMTINGPAAILAAFFMNAAIDQQCERYIKANGLEKEVDNRLKELWEKQKI
                     ARPCYQGDLPEGNDGLGLQLLGITGDRILPKDVYEKIKAHTLSQVRGTVQADILKEDQ
                     AQNTCIFSTEFSLRVMGDVQQYFIDHKVRNFYSVSISGYHIAEAGANPITQLALTVSN
                     GFTFVEYYLSRGMHIDDFAPNLSFFFSNGMDPEYSVLGRVARRIWSKAMKYKYGGNER
                     SQMLKYHIQTSGRSLHAQEIDFNDIRTTLQALYAIYDNCNSLHTNAYDEAITTPTEES
                     VRRAMAIQLIINRELGLAKNENPLQGSFIIEELTDLVEEAVLSEFDRISERGGVLGAM
                     ETMYQRGKIQEESLYYETLKHTGEYPLIGVNTFLSSKGSPTILPKEVIRATKEEKEYQ
                     IAMLNNLHKANAEKSKEMLKKLQDVAVKNGNVFAELMETVKYCSLGQITEALFEVGGQ
                     YRRNM"
BASE COUNT          727 a          741 c          781 g          508 t
ORIGIN      
        1 gacgcccgaa tacggcgcgg cgtcccagct cgagaagatc gacatgctgg atttcgccga
       61 cctcatcgcg ctgaacaagt tcgacaaacg cggcgcgctc gacgccttgc gtgacgtgcg
      121 caagcagtac aagcggaacc accagctgtg gcagacggac gatgacaagc tcccggttgt
      181 gggaacgatc gcttcgcagt tcaatgaccc gggcatgaac catctctacc gcatgctcat
      241 cgacaagatc gcggagaaga ccggcgccga tttgaaatcg cattttgaga tcacgaagga
      301 aatgtcggag aagatctaca tcattcctcc ggcgcggacg cgttacctca gcgagatctc
      361 ggaaaataac cgcggttacg ataagaaagt gaagcagcag gcagatgttg cacaaaagct
      421 gtacgctctc cgcaaagcca tcgacgtgat gtcggcaaac aaagcggccg tcgcttcgct
      481 cgaagaagaa tacaagcgcc tcctcctgga gctcgatccg aaaaacctga agctgatcaa
      541 agagtggccg gaaaaagcgc agcagtacaa aaacgagttc tacgttttca aggtgcgcga
      601 caaagagctg aagataaaaa cgcattacga atcgctttcg catacgctcg ttccgaaaat
      661 agcgacgccg cgttacgaag cctggggcga cctgctccag tggctggcac aggaaaatgt
      721 gccgggcgaa ttcccgtata cgtcgggcat ttacccgttc aagcgcgaag gagaagatcc
      781 tacgcgcatg ttcgccggtg aaggcggacc cgagcgcacg aacaaacgtt tccattacgt
      841 ttcgctgggc atgccggcga aacgattgtc cacggcattc gattccgtga cgctttacgg
      901 gcgcgatccc gattaccgcc cggacattta cgggaagatc ggcaactcgg gcgtgagcat
      961 ctgctgcctc gacgatgcaa agaaattgta ttccggtttc gatctcgccg atccgaaaac
     1021 ttccgtctcg atgaccatca atggtccggc ggcgatactg gcggcgttct tcatgaatgc
     1081 ggcgatcgac cagcagtgcg agcggtacat caaagcgaac ggtcttgaaa aagaggtgga
     1141 caaccgcctg aaggaattgt gggaaaagca gaagatcgcg cggccctgct accagggcga
     1201 tctcccggaa ggaaatgacg gacttggtct gcagctgctc ggcatcaccg gcgaccgtat
     1261 cctgcccaaa gacgtttacg aaaaaataaa agcgcatacg ctttcgcagg tgcgcggaac
     1321 cgtgcaggcg gatattctca aagaagacca ggcgcagaac acgtgcattt tctccacgga
     1381 attctcactg cgcgtaatgg gtgatgtgca gcagtatttc atcgaccaca aagtgcggaa
     1441 cttctattcc gtttccattt ccggctacca catcgcggaa gcgggcgcca acccgatcac
     1501 gcagctcgcg cttacggttt ccaacggctt cacgttcgtg gaatattacc tgtcgcgcgg
     1561 catgcacatc gacgatttcg cgccgaacct ttccttcttc ttctcgaacg gcatggaccc
     1621 ggaatattcc gtgctcggcc gcgtggcgcg gaggatctgg tcgaaagcga tgaaatacaa
     1681 atacggcggc aacgaacgtt cgcagatgct gaagtaccac atccagacat ccggccgttc
     1741 gctgcatgcg caggagatcg acttcaacga catccgcacg acgctgcagg cgctgtacgc
     1801 catttacgac aactgcaatt cgcttcacac gaacgcgtac gacgaagcca tcaccacgcc
     1861 gacggaagaa agcgtgcgcc gcgcgatggc gatacagctc atcatcaacc gcgagctggg
     1921 acttgcgaag aatgaaaacc cgctgcaggg atcgttcatc atcgaagagc tgacggacct
     1981 cgtggaagaa gccgtgcttt cggaattcga ccgcatcagc gaaagaggcg gtgtgctcgg
     2041 tgcgatggaa acgatgtacc agcgcgggaa gatccaggaa gaatcgttgt attacgaaac
     2101 gctcaagcac acgggtgaat acccgctcat cggcgtgaac acgttccttt cctcgaaagg
     2161 ctcgccgacc atcctgccga aagaagtgat ccgcgcgacg aaggaagaga aagaatacca
     2221 gatcgcgatg ctgaataacc tgcacaaggc gaacgcggag aaatcgaaag agatgctgaa
     2281 gaagctgcag gatgtcgcgg tgaagaacgg gaacgttttc gccgagctga tggaaaccgt
     2341 gaagtactgc tcactcggcc agatcacgga agcgctgttc gaagtgggag gacaatacag
     2401 aaggaatatg tgatgtgccg gctgcgcgaa cgatggcgga gcggcgatgg tttttaaacg
     2461 cacggcagta aaacaaccag ccccggcaga ggcgggagcc tgagcctgcg aagcggaagc
     2521 cgatgacggg aacgcggaaa ataaaatgtg atgatggtgc gctccccgta aaaaaagtaa
     2581 cttgcttttc ccggttcaaa atcgaagcac atgcacattg tcgtcaatca cctccatttc
     2641 agcaaacccg ttgatgagct gacggaaaaa ataaagaacg aaggtgttcc gctgctggcg
     2701 cggaatccgg gcttcaaagg gtttcacatg gtgaaagtgg atgagtttca cgctgtc
//
LOCUS       DASTSF010000548         2257 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_551096,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000548 DASTSF010000000
VERSION     DASTSF010000548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2257
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_551096"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..449
                     /locus_tag="VFU15_15640"
     CDS             <1..449
                     /locus_tag="VFU15_15640"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEU4719277.1"
                     /translation="EGTITGNIGRSLRDRKVQDVFPDGDHGKPAVTHWKVLERFGYVT
                     LVECKLETGRTHQIRVHMKSIGHPLFNDTTYGGDRILKGTTFTKYRQFVENCFALCPR
                     QALHAKTLGFVHPKTKKWVEFDSELPADMKALLEKWRAYAQVKPSE"
     gene            complement(488..1153)
                     /gene="hxpB"
                     /locus_tag="VFU15_15645"
     CDS             complement(488..1153)
                     /gene="hxpB"
                     /locus_tag="VFU15_15645"
                     /EC_number="3.1.3.22"
                     /EC_number="3.1.3.50"
                     /EC_number="3.1.3.68"
                     /inference="COORDINATES: protein motif:HMM:NF008087.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HEU4719278.1"
                     /translation="MTEAVIFDMDGLLVDSEPYWRVAEQNVFGRLSKAPGENEFEKMM
                     GNRIQEVIRSWYEKNPWENFSPEQTQQEIVEEVHRLVIAHASLLPGVQEIFSFFRSRN
                     IPVALASSSPYALIESLMHHYGLIGHFKTVHSAENEPYGKPHPAVFITTAYKLGIDPQ
                     KCLVFEDSFNGIIAAKAARMKCVAVPAKEHFAQERFSAADMKLKSLLEFTEEKWRAVS
                     DEY"
     gene            complement(1456..>2257)
                     /locus_tag="VFU15_15650"
     CDS             complement(1456..>2257)
                     /locus_tag="VFU15_15650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CofH family radical SAM protein"
                     /protein_id="HEU4719279.1"
                     /translation="VTEVHIVGGVHPKLGLDFFGGVLEKIKQIIPGIHIKGFTAVELE
                     YMFRKGKVSVEEGLKFLKSKGLDSLPGGGAEIFDETIREQICADKCTSEEWLNIHETA
                     HRLGIPSNATMLYGHIESFAHRIDHMERLRQLQDRTKGFNTFIPLKFRNRDNQMENVP
                     EVSVIEDMRNYAISRIYLDNFAHIKAYWPMLGRTNAQLSLSFGVDDIDGTIDDSTKIY
                     SMAGAEEQNPALTTQQLVDLIAAVRRKPIERDTLYHVLHDYSVEKVEF"
BASE COUNT          534 a          565 c          593 g          565 t
ORIGIN      
        1 acgaaggaac gatcaccggg aatatcgggc ggagcttgcg cgaccggaaa gtgcaggatg
       61 tttttcccga tggcgatcac gggaaaccgg ccgtgacgca ttggaaagtg ctcgaacgtt
      121 tcggatacgt cacacttgtc gaatgcaagc tggaaacagg ccgcacgcac cagatacgcg
      181 tgcacatgaa atcgatcggc cacccgcttt tcaatgatac gacgtatggc ggcgaccgca
      241 tcctgaaagg aacgacgttc acgaagtaca ggcagttcgt cgaaaactgc tttgcgttat
      301 gcccgcgaca ggcgctgcat gcgaagacgc tgggctttgt tcacccgaag acgaagaaat
      361 gggtggaatt cgattcagag cttccggcgg atatgaaagc gctgctggaa aaatggagag
      421 cctatgcgca ggtgaaaccg tcggaataga ttacggttgg aaagttttat ttcctgtccg
      481 cacccgctca atactcatca ctgaccgccc gccacttttc ctccgtgaat tcgaggagcg
      541 acttcagctt catgtcggcc gcagaaaacc tttcctgtgc aaaatgttct ttcgcaggca
      601 ccgcgacgca tttcatcctg gccgctttcg cagcgatgat gccattgaac gaatcctcga
      661 aaacgagaca tttttgcgga tcaataccga gcttgtacgc tgtggtgatg aaaacagccg
      721 ggtgcggttt gccgtaaggt tcgttctctg ccgaatgaac ggttttgaaa tgaccgatca
      781 gtccgtaatg atgcatgagc gattcgatga gtgcatacgg ggaggaagag gcaagcgcga
      841 cgggaatatt ccgggaacgg aaaaagctga aaatttcctg tacacccggg agcagcgacg
      901 catgcgcgat gacgaggcga tgaacttcct ccacgatctc ctgctgcgtc tgttccgggg
      961 aaaaattctc ccacggattt ttttcatacc aggagcggat cacttcctgg atgcggttgc
     1021 ccatcatctt ttcaaattcg ttttcaccgg gcgctttgct cagccgcccg aatacgtttt
     1081 gttcggcgac tctccagtaa ggttcggaat cgaccagcaa accgtccatg tcgaagatca
     1141 cagcttcagt catggcgcga atttagggca taaaaaaacc ggtcggctgt tttcccccat
     1201 gcgcgaccgg ttttaaacgt gaagacttcg gtgtgaagtg ctacaggacc cttctgtgca
     1261 ccatcatttt ttggaaagga tttgacaaca ccgagatgac tatcacacca caaaactatc
     1321 cgtaggatag ttttgacaag cccaaatatg aagaaaagtg aagacagccg attcgggcct
     1381 tattttttat aaatatttga tatttaatat tttgaatgcc tgaaaaaaga caaccgatcc
     1441 cggatttttc agattttaaa attccacttt ttcgaccgaa taatcatgca ggacatggta
     1501 caacgtatcc ctttcaatgg gtttccgcct gacggcggcg ataaggtcga caagctgctg
     1561 cgtggtaagc gccggattct gctcttcggc accggccatg gaatagatct tcgtggaatc
     1621 gtcgatcgtt ccgtctatat cgtcaacgcc gaagctgagc gaaagctgtg cgttcgtgcg
     1681 accgagcatg ggccagtacg cttttatgtg cgcgaaatta tcgaggtaaa tgcggctgat
     1741 cgcgtagttg cgcatgtctt cgatcacgga aacttccggc acgttttcca tctggttgtc
     1801 gcggttgcgg aatttcaacg gaatgaatgt gttgaatcct ttcgtgcggt cctgcagctg
     1861 cctcagccgt tccatgtgat cgatgcggtg cgcgaatgat tcgatgtgcc cgtacagcat
     1921 ggtggcgttc gaaggaatgc cgagcctgtg cgccgtttcg tggatgttca gccattcttc
     1981 ggatgtgcat ttgtcggcgc agatctgttc ccgtatggtt tcgtcaaaga tttctgcacc
     2041 gccgccggga agcgaatcga gtcctttcga tttcaggaat ttcaatcctt cttccaccga
     2101 aacttttccc ttgcggaaca tgtattccag ttccaccgcg gtgaatcctt tgatgtggat
     2161 gccgggaatg atctgcttta ttttttcgag tacgccgccg aaaaaatcga gtccaagctt
     2221 cgggtgaacg ccgcccacga tgtgcacttc ggtcacc
//
LOCUS       DASTSF010000549         4296 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1639892,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000549 DASTSF010000000
VERSION     DASTSF010000549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4296
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1639892"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..535
                     /locus_tag="VFU15_15655"
     CDS             <1..535
                     /locus_tag="VFU15_15655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719280.1"
                     /translation="LLVAAHYLAGVKYPIQRMGLYIFVLMMFALVAVLPQLLPQRSAT
                     VAGICIALPVVFHFAYCANFSYLCEWKACAGAQRAAEEIMQRCKDIPAGRQVRVCGDG
                     YSGSAIEFVAERDYPGRMNVFINWSEDQPAQYDFYLIEKWAIPSFDQSQWIAIDTMPY
                     SENVLFMDSSFVRQEKK"
     gene            complement(555..941)
                     /locus_tag="VFU15_15660"
     CDS             complement(555..941)
                     /locus_tag="VFU15_15660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719281.1"
                     /translation="MKRFSLFLIFLVPFLQIRAQHVSGKIKSTGNADLHVIRLYADSF
                     PKVDLVFQAEDTLGNPVWNLAQKDVRVEEDGRQGRITSFQLLSEEQHLQTMLVIDHSA
                     LDDGEYLVRLESTAAVSGTKKLIVRH"
     gene            complement(1104..3923)
                     /gene="carB"
                     /locus_tag="VFU15_15665"
     CDS             complement(1104..3923)
                     /gene="carB"
                     /locus_tag="VFU15_15665"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011586628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEU4719282.1"
                     /translation="MPKDNSLKHVLIIGSGPIVIGQACEFDYSGSQAARSLRQEGIEV
                     TLINSNPATIMTDKVTADNIYLLPLTTQSIVKILEEHTIDAVLPTMGGQTALNLALKC
                     DELGIWKKYNVRMIGVDIDAIRVTEDRDMFRARMESIGVAMAPSRIAKSFLEGKGIAQ
                     EFGFPLVIRPSFTLGGSGGSVVYVKDDFDKALNRGLQTSPIHEVLIDKAVLGWKEFEL
                     ELLRDANDNVCIICSIENFDPMGVHTGDSITVAPAMTLSDTTFQRMRTMAIKMMRSIG
                     NFAGGCNVQFAVSPDEKEEIIAVEINPRVSRSSALASKATGYPIAKIASKLAIGYHLD
                     ELMNQITQSTSAFFEPTLDYVIVKIPRWNFDKFKGTNRELGFQMKSVGEVMAIGRSFQ
                     EALQKAAQSLEIKRNGLGADGKEITNQQELLNALERPSWNRLFMIYDAIKLGISVKTI
                     HKLTKIDPWFLNQIEELVAIEKEIEKYGIGTIPKELMFEAKQKGYADRQIAHLLRCLE
                     SEVYKKRAEEFNIRRVYKLVDTCAAEFEARTPYYYSTFETENESVRSEKKKVIVLGSG
                     PNRIGQGIEFDYSCVHGVLAAKESGYETIMINCNPETVSTDFNVADKLYFEPVFWEHI
                     YEIIQHEKPEGVIVQLGGQTALKLAEKLDRYGIKIIGTDFRSLDLAEDRGSFSTLLRD
                     NHIPYPKFGVIEDADQAIELSRELGFPLLVRPSYVLGGQSMKIVLNEQELEQHVVKLL
                     NDIPGNKVLLDHFLDGAIEAEADAICDGKDVYIIGVMEHIEPAGIHSGDSYAVLPPFD
                     LSENVLKQIEAHTKKIALALNTVGLINIQFAVKNEIVYIIEANPRASRTVPFIAKAYD
                     EPYVNYATKLMLGVNKVKDFNFNPRKKGYAIKIPVFSYEKFPDVQKELGPEMKSTGEA
                     IYFIDDLHDEYFHQIYSERNLYLSK"
BASE COUNT          883 a         1162 c         1174 g         1077 t
ORIGIN      
        1 gctccttgtt gccgcgcatt acctcgccgg ggtgaaatat cccatccagc gcatgggtct
       61 ttacattttc gtgctgatga tgttcgcgct ggtcgctgtt ctgccgcagc tgcttccgca
      121 acgctcggca acggtggccg gcatctgcat agctctcccg gttgtttttc atttcgctta
      181 ttgcgcgaat ttcagttacc tctgcgaatg gaaagcctgt gcaggtgcac aacgcgctgc
      241 ggaagaaatc atgcaacgct gcaaagatat tcccgcaggc cggcaggtgc gtgtgtgcgg
      301 agacggatat tcgggcagcg cgattgaatt cgtcgcggag cgggattatc ccgggcgcat
      361 gaatgtcttc atcaactgga gcgaagacca gccggcgcaa tacgattttt acctcatcga
      421 aaaatgggcg atcccttcgt tcgatcaatc gcaatggatc gcgatcgata cgatgccgta
      481 ttcggaaaat gtgctgttca tggattcgtc gtttgtccgg caggaaaaaa aataatttcc
      541 gtccttccgt tttctcagtg acgcacgatg agcttcttcg ttccggaaac agccgctgtc
      601 gattccagcc tgacgagata ttctccatcg tccaacgcgg aatggtcaat caccagcatg
      661 gtctgaagat gctgttcttc cgaaagcagc tggaaagagg tgatccttcc ctgccgtccg
      721 tcttcttcga cacgcacgtc cttctgcgcg aggttccaca cgggattacc cagcgtgtct
      781 tccgcctgga aaacaagatc gaccttcgga aaagagtctg catacagccg gatcacgtgg
      841 agatccgcgt tgccggtgga ttttatcttt ccgctgacgt gttgagcgcg gatttgcagg
      901 aaaggaacaa ggaagatgag gaaaagactg aagcgtttca tggcggttat tttccggtta
      961 ataccatgaa gacgcagaag gtaacccggc gaaataaaat tttcaaccgg aactggcaag
     1021 tggccgttcc agaccggcag ccggatcacg gttgcgaagt ttcgcaggcc gaaacagcag
     1081 gataatacgg gaaactcccg ggcttatttc gagagataca ggttccgttc cgaatagatc
     1141 tggtggaaat attcatcgtg caggtcgtcg atgaaataga tcgcttcgcc cgttgatttc
     1201 atttcaggtc caagctcctt ctgcacgtcg gggaatttct cgtacgagaa aaccgggatt
     1261 ttaatggcgt atcctttttt gcgcgggttg aagttgaaat ccttcacctt gttcacgccg
     1321 agcatgagct tcgtggcgta attcacgtac ggctcgtcgt aggctttcgc gatgaacgga
     1381 accgtgcgcg atgcacgcgg gttcgcttcg atgatgtaca cgatctcgtt cttcacggcg
     1441 aactggatgt tgatcagccc gaccgtgttc agcgcgagcg cgatcttctt cgtgtgcgcc
     1501 tctatctgct tcagcacgtt ttccgaaaga tcgaaaggag gcagcacggc atacgaatcg
     1561 ccggagtgga taccggcagg ctcgatgtgc tccatcacgc cgatgatgta aacgtccttc
     1621 ccgtcgcaga tcgcgtctgc ttccgcttcg atcgcaccgt cgaggaaatg gtcgagcagg
     1681 actttgttgc cgggaatatc gttgagcagc ttcacgacgt gctgctcgag ctcctgttcg
     1741 ttcagcacga tcttcatgct ttgtccgccg agcacgtacg aaggacgcac cagcagcggg
     1801 aacccgagct cgcgcgaaag ttcgatcgcc tggtcggcat cttcgatcac accgaatttc
     1861 gggtacggga tatggttgtc gcgcagcagc gtggaaaaac ttccgcggtc ttccgcaaga
     1921 tccagcgatc tgaaatccgt tccgatgatc tttattccgt aacggtcgag cttctcggcc
     1981 agcttcagcg cggtctgccc gccgagctgc acgatcacgc cttccggttt ttcgtgctgg
     2041 atgatctcgt aaatgtgttc ccagaaaacc ggctcgaaat aaagtttgtc cgccacgttg
     2101 aaatcggtgg aaacggtttc cgggttgcag ttgatcatga tcgtttcgta gccgctttcc
     2161 ttcgcagcga gtacgccgtg cacgcaggaa taatcgaact cgatcccctg cccgatcctg
     2221 ttcggacccg atccgaggac gatcactttt ttcttttccg aacggacgga ttcattttcc
     2281 gtctcgaacg tggaataata atacggcgtg cgcgcttcga attccgcggc gcaggtatcg
     2341 acgagcttgt acacgcggcg gatattgaat tcctccgcgc gctttttgta cacttcactt
     2401 tcgagacagc gcagcaggtg cgcgatctgg cggtcggcat aacctttctg cttcgcctcg
     2461 aacatcagct ctttcgggat cgtgccgatg ccgtatttct cgatctcttt ttcgatcgcc
     2521 acaagttctt cgatctggtt gaggaaccag ggatcgatct tcgtcagctt atggatcgtt
     2581 ttcacggaaa tgccgagctt gatcgcgtcg tagatcatga acaggcggtt ccacgacggg
     2641 cgttcgagcg cgttgagcag ctcctgctgg ttggtgatct ctttcccgtc ggcgcccagc
     2701 ccgttgcgtt tgatctcgag gctctgcgcg gctttctgca gcgcttcctg gaagctcctg
     2761 ccgatcgcca tcacttcgcc tacggatttc atctggaagc cgagctcgcg gttcgtgcct
     2821 ttgaacttgt cgaaattcca gcgcgggatc ttcacgatca cgtagtcgag cgtgggctcg
     2881 aaaaacgccg acgtgctctg cgtgatctgg ttcatcagct cgtcgaggtg gtagccgatg
     2941 gccagcttcg acgcgatctt cgcaatggga tagcccgttg ctttcgaagc aagcgcagac
     3001 gaacgtgaaa cacgcgggtt gatctcgaca gcgatgatct cttctttttc atccgggctt
     3061 acggcaaact gcacgttgca gccgcccgca aaattcccga tggagcgcat catttttatc
     3121 gccatcgtac gcatgcgctg gaacgtcgta tccgaaagcg tcatcgcagg cgccaccgta
     3181 atggaatcgc cggtgtgcac gcccatcggg tcgaaattct cgatggagca gatgatgcac
     3241 acattgtcgt tcgcgtcacg cagcagctcg agctcgaact ctttccagcc gagcacggct
     3301 ttatcgatga gcacttcgtg aatgggggat gtctgcagtc cgcggttgag cgctttgtca
     3361 aaatcatctt tgacgtacac cacggatccg ccgctcccgc caagcgtgaa tgacgggcgg
     3421 atgacgagcg ggaaaccgaa ttcctgcgca atgccttttc cctcgaggaa tgatttcgcg
     3481 atgcgcgaag gcgccatagc cacgccaatg ctttccatgc gcgcacggaa catgtcgcgg
     3541 tcttccgtca cgcggatcgc atcgatgtcg acaccgatca tgcgtacgtt gtatttcttc
     3601 cagatgccga gctcgtcgca cttgagcgcg aggttgagcg ccgtctgccc gcccatggtg
     3661 ggaagcaccg catcgatcgt atgctcttcg aggattttca cgatcgactg ggtggtaagc
     3721 ggcagcaggt aaatattgtc ggccgtcact ttgtcggtca tgatggtggc cgggttgctg
     3781 ttgatcagcg tcacctcgat gccttcctgg cgcagcgagc gcgcggcctg tgagccggag
     3841 taatcgaatt cgcaggcctg cccgatgacg atgggaccgc tgccgatgat gaggacgtgt
     3901 ttgagggaat tgtctttagg catggcgagt gtaaaaaaat cacgcgaagt tagggcataa
     3961 cacgcggtga aaggccaaaa cttttacacg ggtgttagta aaatgccgga acagccgtca
     4021 cgcgcacaac gcaggactgg caagggattg agcgattcaa attccttctt cgcattccgt
     4081 ttttttgatg taaatttgtc ctttgtttct tgttccgcct gtcaatcctc cagcctgttt
     4141 tcaatacgta tcctgagacg gttagcatgt ccccggcttt gcgaccaaat gataaaagag
     4201 ctttaaccgc gtggatattt atcctttgcc tggcaattga aaggtttacg ttctcaatga
     4261 tctcttaccg gacttccgcc actttcagat ctcatt
//
LOCUS       DASTSF010000550         2564 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1666976,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000550 DASTSF010000000
VERSION     DASTSF010000550.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2564
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1666976"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..928
                     /locus_tag="VFU15_15670"
     CDS             <1..928
                     /locus_tag="VFU15_15670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719283.1"
                     /translation="VRQQLRSKAYLENALAVNTLGIRDIFLRDPVPAIERYRRLLDAW
                     RNKKEWQEDQSDLLLTICKYYQNACFFSTVSPDIVHADLNSLKGFDGLPPEKLRTFRE
                     TMFHHAFILSLNGGKLNSALRMIPVISSWMDKEEKHLSQAQVLPFLCNFMVAEFLSGH
                     FAEANKHLNRILQLPYRNVRVDIREFALMLQPFVQYELDNTGLNEYLTRSRRRHFSKN
                     RKTHDFEMIVIKHIELLLRAGDAAQKKKVLQSFISSLEKISESQSRAVPLLGLNEIRM
                     WAISRKNNIPLSAVFVGEVKKAHERIDSDKSR"
     gene            1101..>2564
                     /locus_tag="VFU15_15675"
     CDS             1101..>2564
                     /locus_tag="VFU15_15675"
                     /inference="COORDINATES: protein motif:HMM:NF026255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEU4719284.1"
                     /translation="MKKIFFSLFLPVFLLPLHTHAQVTGTVTDSLGQPLPFCNVMLLN
                     AADSSVVTGTTGDDKGSFSLEKKRDGNFLVMAIFSGYEKYYSLPFSLSETQPKYNAGT
                     IVLRADPKLLGSVDIVAQKPFMEQKLDRVVFNIDNSVVASGSNALELLKKLPGVMVDN
                     NDNIQVRGKPGVQIMINGRTSYLSGGDIANYLKSIDASQVERIEVITNPSAKYDAAGN
                     AIINIVLKTNKNMGLNGQVNVNSGQGFYNETDVNLNLDYRMKKWNFYVNQSNSEGRFY
                     DQAHRTATFSQGGTPNAVFESDQRIFYHGHWNYTNAGVDFSPGTRQTIGASAEFSGGS
                     RVQNRTFSVDMYDGNSVLDSSLFTNGAQRDTQLYLTYSMYYQFRIDTNGRELSADISY
                     APFSTTASRTNTTSYEYANSASRQPSVLFYRLPVNVDIRAGQIDYVQPFGQNTKFETG
                     AKASYVSTDNNARYYNVVQDVYSEDTTLSNHFLYTEKI"
BASE COUNT          639 a          670 c          667 g          588 t
ORIGIN      
        1 ggtccggcag cagctccgtt caaaagcata cctggaaaat gcgcttgccg tgaatacgct
       61 cggcatccgt gatatcttcc ttcgcgaccc tgttcccgcg atcgagcgtt accggcggct
      121 gctcgacgcc tggcggaaca aaaaagaatg gcaggaagat cagtcggatc ttctccttac
      181 gatctgcaag tattaccaga atgcctgctt cttttctacg gtaagtcctg acatcgtgca
      241 tgccgatctc aattcgctga aaggcttcga cggtctgccg cccgaaaagc tgcgcacatt
      301 ccgcgaaacg atgttccacc acgcattcat tctcagtctc aacgggggaa agctcaactc
      361 cgcgctccgg atgatcccgg tgatcagcag ctggatggat aaagaggaaa aacatctttc
      421 gcaggcgcag gtgctgcctt tcctctgcaa tttcatggtg gctgaatttc tttccggtca
      481 ttttgcagag gcgaacaaac acctgaacag gatcctgcag ctgccgtacc ggaatgtgcg
      541 ggtggatatc cgcgagtttg cgcttatgct tcagcctttc gtgcagtacg agctcgacaa
      601 tacgggattg aacgagtacc tgacgcgttc gcgcaggagg catttcagca aaaacaggaa
      661 aacgcacgat ttcgaaatga ttgttataaa acacatcgag ctgctgctgc gtgcaggaga
      721 tgccgcgcaa aagaagaaag tgctgcaaag cttcatctcc tcgctggaaa agatcagcga
      781 gagccagtcg cgtgcagttc ccctgctggg actgaatgag atccgcatgt gggccatctc
      841 gcggaaaaac aatattcctc tttccgctgt ttttgtgggg gaagtgaaga aagcgcatga
      901 acgcatcgac agtgataaat cccggtgaaa aaatgacggg cggcccgggc ttcgttttgc
      961 gacaaaatca accttaacac tttttatcct ttctgcgtta ttattgggaa atccggcgga
     1021 gggcttttct tttctttgcc gtgttcgttg acacgaaaaa gtttttttaa agtaacctca
     1081 accactaccg ctccgcattt atgaaaaaaa ttttcttttc cttattcctg cccgttttcc
     1141 tgttgccgct gcacacgcat gcgcaggtca ccggaacagt taccgactca ctcggccagc
     1201 cgcttccatt ctgcaacgtg atgctcctga atgctgccga cagttcggtg gtgaccggga
     1261 ccacgggcga cgacaaagga agtttttcgc ttgagaaaaa gcgcgacggg aattttctcg
     1321 tgatggcgat cttcagcggg tatgaaaaat attattccct tcccttttcg ctttcggaaa
     1381 cgcagccgaa atacaatgca ggcacgatcg ttcttcgcgc tgatccgaaa ttgctgggca
     1441 gcgtggacat tgttgcgcag aagccgttca tggagcaaaa actcgaccgc gtggttttta
     1501 atatcgacaa cagcgttgtc gcatcgggga gcaatgcgct ggaactcctg aaaaagctgc
     1561 cgggtgtgat ggtcgacaac aacgacaaca tccaggtgcg cggaaagccg ggtgtgcaga
     1621 tcatgatcaa cgggcgaacg tcgtacctgt caggcggcga catagcaaat tacctgaaaa
     1681 gcattgacgc ttcgcaggtg gaacgcattg aagtgatcac aaacccttct gcgaagtacg
     1741 acgctgcagg caacgcgatc atcaacatcg tgctgaagac gaataagaac atggggctta
     1801 acgggcaggt gaatgtcaac agcggccagg gattttacaa tgaaacggat gtgaacctga
     1861 acctggatta ccgcatgaag aaatggaatt tctacgtcaa ccagtcgaac agcgagggcc
     1921 ggttctacga ccaggcgcac cgtacggcga cgttcagcca gggcggaacg ccaaacgcgg
     1981 tatttgaaag cgaccagcgc attttttatc acggtcattg gaattacaca aacgcaggtg
     2041 tcgatttttc accgggaacc cggcaaacga tcggagcttc cgcggaattt tccggcggat
     2101 cgcgcgtgca gaaccggacg ttcagcgtgg atatgtacga tggaaattcg gtgctcgatt
     2161 cgagcctctt cacgaatggc gcgcagcgcg acacgcagct gtatctcacg tacagcatgt
     2221 attaccagtt ccgcatcgat acgaacgggc gtgagctcag cgcggatatc agctatgcgc
     2281 ctttctccac cactgcctcg cgcacgaaca cgacttcgta cgagtatgcg aattctgctt
     2341 cacggcagcc gtccgtcctg ttctatcgcc ttccggtgaa cgtcgatata cgcgccgggc
     2401 agatcgatta cgttcagcct ttcggtcaga acacaaaatt tgaaacgggc gcaaaagcca
     2461 gctacgtttc cacggacaac aatgcgcgct attataatgt ggtgcaggat gtttattccg
     2521 aagacacgac gctttccaat catttcctgt acacggaaaa gatc
//
LOCUS       DASTSF010000551        12310 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1445171,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000551 DASTSF010000000
VERSION     DASTSF010000551.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 12310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12310
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1445171"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..474)
                     /locus_tag="VFU15_15680"
     CDS             complement(<1..474)
                     /locus_tag="VFU15_15680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020080654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU4719285.1"
                     /translation="MTNSPYKILLVDDEQDILEFLNYNLKKEGYQVLTALNGEEAIRI
                     AKKELPHLVLLDVMMPGLDGIEVCRELRNINGSENVIIAFLTARSEDYSHIAGFEAGA
                     DDYINKPIKPRVLISRIKALLRRFPGEKDNANSVDLGGLKIDRDSYLVIKGDDEIS"
     gene            651..3515
                     /locus_tag="VFU15_15685"
     CDS             651..3515
                     /locus_tag="VFU15_15685"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEU4719286.1"
                     /translation="MERKKNGFAFSLFVILALALLVPHFAGAQGGTVSGKVSDKVTGE
                     TMPGVVVLLDSTGKKGAVTDFDGNYTINGVEAGGHSLTFKFISYQTLKVTDVNVSSGK
                     TTTVNAVMELALDTSNIVVIKSSRVTNSEAAVTDEMHNNNNVSSGISGQQISKSKDRD
                     ASEVVKRIPGVTVIDNRFIMVRGLNERYNNVWLNDAGAPSSETDKRAFSFDMVPSGTL
                     DRIMVYKTPSPDLPGDFAGGMVRIYTKAFPEKTILNFSFESNYRAGTTFRDFNYTDGT
                     SKDIFGMGAAMRHIPDGLPAYISRNDPNYTQISQAFPNNWALHTRQALPDMRFSMMWA
                     QPFTMGKKITAGNTLGLGYSRTLTTFSIRRQDWDSTAQISDYTDIQCTDQVRVSAVEN
                     FSMLIGRQKVEFKNLLNQIGKSYSTYRYSNYADGPNEKSYVFGYESRTIYSSQLAGTF
                     KGPKERSVYSFTGGYSYLLKDAPDLRRIAYTKQQNAPDSMYKAGISTIVDPVNGGGRL
                     YQWLQEQTWSLQQNFLQKVNLFDTTIEINIGTYSEYKTRQFIARVLGYTINPGFTAFN
                     LTREPLETIFSPDNVGGFSTFRVDEITNPSDKYTAQNLLLAGYGMAKIPIGKHLAVIG
                     GLRYEYNEQSLQGYVNLDSISPKVITRFYLPSVNMTYNFNRKTLIRAAYGKTVNRPEF
                     REWSPFYFYDFEFKAGTYGSLFPTVVAQKGQLLDVARVDNYDLRFEFYPNAGDYIHVG
                     VFYKSFVEPIQQIILNSGGADSRAFTFVNADYAFVEGAELDMRKNLAVFDTLFHTSVF
                     GNFNTILNVSVMKSRLYLDPSQVVNQSAATPLQGQSPYVVNAGIYYQNDSIGLQASLL
                     YNVFGPRLYLVGTLDYANIGELPRNSFDFVVTQRITPWLSVSGGVQDIFNQPVQMVQD
                     TDRNGKFERNGQDKEILNYRRGRYYTIGITFSPQLNKGGK"
     gene            3649..6711
                     /locus_tag="VFU15_15690"
     CDS             3649..6711
                     /locus_tag="VFU15_15690"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEU4719287.1"
                     /translation="MKQRSTKAITKMFAVVVGMFAAAGAVNAQSVINVQDSIYTNTHW
                     TACNQYLLHGYVYVTAGTTLTIDPGTIIKGDKNTKGTLIIERGAMINAQGTATQPIIF
                     TSNQPAGQRTYGDWGGVILVGRAPNNWLSGQNQVEGGPRSLYGGNNIHDNSGVMSYVR
                     IEFPGVAFSPNNEVNGLSIYSVGDATQIDHIQVSYSGDDSYEWFGGTVNAKYLVSYRP
                     WDDSFDQDCGWRGHVQFAFDIRDPYAADVSGSHAMEMDSYQSGTKSGLAGDTSGLTMP
                     VWSNVTLVGPMVSPTTTSYDPQYVSGIHSRRGNATSVLNSVIIGYPCGILFDESSASY
                     GSTTRNLRILGSAGDSIMQYRGDVVCGIPANGSPVKKELEYVIDGARSLTPTNTEGDT
                     TTGAPFGTWSGPYNFYLTPAYKNKIYPTEQTGISLHSPFNLASPDPTPTSVSPICYNS
                     TSLPGYVTAHYMACCSQSDHFGNGNKYPFNPSLPLNTDTSNFFANYNAPSIVPQWNSG
                     RLAGGFFTPVNYVGAFAGTQSTTDNWMNGWCNFDCENTDYSLTSAPAASITALGNLTI
                     CPSGGSVTLQANAGSGFTYQWMMNSANISGATSQQYTATTGGTYQVTVTNATGCSTTS
                     SSVVVNTFAAPSASITAGGPTTFCAGNNVTLTASGASSYSWSNGATTPSITANTSGNY
                     LVTITDANGCQATSSAVNVVVNPLPNASVTANGPTTFCTGDSVVLTANSSSSYMWSDN
                     STTQSITVYNSGNYSVMVTDANGCQSASSTPITVSSSPSPQPTITIGGNTSLCQGQTV
                     MLTSSTADSYLWSNGATTQSITVSASGNYTVTVTNANACSGTGASNPVAVTVNPNPTA
                     NFTTGGNIPTVNFTNTSTGATSYMWDFGDFSSSSLSSPSHTYGTNGNYTACLVAMSAA
                     GCSDTTCMPVLINVGMSQPILVSDNYNLYPNPANSQLNIAANLTQDRDLQVVAFDVNG
                     KLLINENRSLPAGNNVLTYDVSNWDNGIYFIRVMEGQNVQTMKVIINR"
     gene            complement(7384..8100)
                     /locus_tag="VFU15_15695"
     CDS             complement(7384..8100)
                     /locus_tag="VFU15_15695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719288.1"
                     /translation="MKKHYLPVFVLAGMMAACSGNNAEQQTDHSVTKAILDTQAQPQP
                     QMPADPPAVKNDSAAGQPATQIACYYFMGNSSHDAPPEKTAEKWVDDFISKMKTDGLP
                     HAGNVFLHNGAGPMGAEWNPHSDLLVAGFFEGRKDTSDIHFFVDDFNFDGKTVFSGKY
                     YSPEKNGVYYWFSLTEQNWNVGRHLPSDKEKTEICAQRNIPVSSWVDNTCTVARIAVT
                     AGKGKEMIRSQDYFIVAFGE"
     gene            complement(8145..8849)
                     /locus_tag="VFU15_15700"
     CDS             complement(8145..8849)
                     /locus_tag="VFU15_15700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010418988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU4719289.1"
                     /translation="MSATTLNGSKDSYRVLLVDDDEHILLSLKSILENAGYNVWCSDN
                     TREAIELATRIQPHLIILDVIMPGTDGIELCQEIRQIPVLSKTLIVFYTARSEDYSQI
                     AGFSAGADDYIIKPMKPGVLVARVAALLRRFRLRELPKTAQAGPIRLDRERYLVFKGQ
                     KEIVLARKEFELLALMLSSPRKVFTRNEIYSEIWGGEFGEKNRTIDVHVRKLREKIGE
                     EFIKTVKGIGYSFDVV"
     gene            9107..9451
                     /locus_tag="VFU15_15705"
     CDS             9107..9451
                     /locus_tag="VFU15_15705"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009578628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HEU4719290.1"
                     /translation="MKGKPESDFFYMVYEVVRLVPFGRVTTYGAIAKYLGMKGSSRMV
                     GWAMNSSHSHHSPVPAHRVVNREGMLTGKNHFSTPHRMQELLEKEGIKVVKDQVQNFK
                     NHYWDPSEELKL"
     gene            complement(9458..9925)
                     /locus_tag="VFU15_15710"
     CDS             complement(9458..9925)
                     /locus_tag="VFU15_15710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719291.1"
                     /translation="MKQFLAAVLFAVCISVQAQKSYLVHLTIDKLFLGSDSVVYPKGK
                     TFVMNITSKSDTVEVGKPGGVPVAILVDIRRIKRGDRIINQVGYAFLQKTGGKWTLIK
                     HFGYTDRYDFPTHKPGFENTARKTQAKDEFHCDYGVPRQFAADFRMDIYRNDN"
     gene            9999..11075
                     /locus_tag="VFU15_15715"
     CDS             9999..11075
                     /locus_tag="VFU15_15715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018479655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140663 - ATP-dependent FeS chaperone
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HEU4719292.1"
                     /translation="MITTEQILDALRNVDDPDLKKDIVTLGMVKDIHVDGKKVAFTVV
                     LTTPACPLKDMIRRACENAVKYYVDKEAEVLVNMTAHVTTKRKEPSMPGVKHIIAVAS
                     GKGGVGKSTIAANLACALAMQGASVGLVDADIYGPSIPIMFDVVNEKPRIQNIDGRNR
                     MIPVESYGVKILSIGFFADANQAIVWRGPMAVKALNQMLNDAEWGELDYMIIDLPPGT
                     GDIHLSIVGAVPITGAIVVSTPQNVALADARKGVAMFRMEQIHVPVLGIVENMAWFTP
                     AELPENKYYIFGREGATRLAEDLQVPLLGQIPLVQSICEAGDAGRPAVLQKDTPQAKA
                     ILEMAGNVVRRVAVMDAAKAEVIA"
     gene            11113..11373
                     /locus_tag="VFU15_15720"
     CDS             11113..11373
                     /locus_tag="VFU15_15720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014682349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HEU4719293.1"
                     /translation="MNAALTERVEAALNQLRPFLEADGGGVTLLEITADNVVKLELQG
                     ACRSCSMRMMTFKAGLEEAVKRAAPEIVAVEAINLVDDGQPV"
     gene            11578..>12310
                     /locus_tag="VFU15_15725"
     CDS             11578..>12310
                     /locus_tag="VFU15_15725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HEU4719294.1"
                     /translation="MSTGTQEIKKEAVILFAGDSGDGIQLTGSQFTGTAAFYGNDVST
                     FPNFPAEIRAPQGTLAGVSGFQLHFGSMEINSPGDACDVLVVMNAAALKANLRQLKKH
                     GTIIANTDGFDPKNLKLAKYADGDNPLKNGSLSDYHVIGIDVTKLTRNALSGSGLGTK
                     ETDRSKNMFVLGFIYWMYNRKPDLTVEFLKEQFKKRPELIDANVKVLMAGYHYGDTTE
                     TPMTRYEIKAANLPPGTYRSITGNQG"
BASE COUNT         2979 a         3388 c         3167 g         2776 t
ORIGIN      
        1 tgaaatttca tcgtcgcctt ttatcacgag gtagctgtcg cggtcgatct tgagaccacc
       61 gagatcgacg gagttcgcat tgtctttttc gccgggaaag cggcggagca gcgccttgat
      121 gcggctgatg agcacgcgcg gcttgatggg cttgttgatg taatcgtcgg ccccggcttc
      181 aaaaccggcg atgtgcgaat aatcctcgct gcgcgccgta aggaatgcga tgatcacatt
      241 ttcggaacca ttgatattgc gcagctcccg gcacacttcg atgccgtcga gaccgggcat
      301 catcacatcg agcagcacaa gatgagggag ctcttttttg gcaatgcgga tagcctcttc
      361 accattcaac gccgtcagca cctggtatcc ttcctttttc aggttgtaat tcaggaactc
      421 caggatgtcc tgctcatcgt ctaccagcaa aattttatat ggagaattag tcatagaatc
      481 ttcactgatg caaatgtaaa ccggctcttc gccgacaatg ttaacgtaaa attaagttaa
      541 tctgaactgg tggcggctgc cgtaattggc agcactttac atctaattca tactcgatta
      601 atgcaaaggt aaggttgtgc gggttctttt gctgttcaat aaaacgtttt atggaaagga
      661 aaaagaacgg gtttgcattt tctttgttcg tcattctcgc tttggcgttg ctggtccccc
      721 atttcgcagg ggcgcaggga ggcacggtct cgggaaaagt gtcggacaaa gttacgggag
      781 aaaccatgcc gggtgtcgtt gttcttcttg acagcacagg aaaaaaaggc gcggtaaccg
      841 atttcgacgg caactacacg atcaacggtg ttgaagccgg cggacattcg ctcacgttca
      901 agttcatttc ctaccagacg ctcaaggtga cggacgtcaa tgtcagttcc ggcaaaacaa
      961 ccaccgtgaa tgcggttatg gagctggcgc tcgacacgag caacattgtc gtcatcaaaa
     1021 gttcgcgcgt gacaaactcc gaagcggcag tgaccgatga gatgcacaac aacaacaatg
     1081 tatcgagcgg aataagcggg cagcagatca gcaaaagcaa agaccgtgat gcgtcggaag
     1141 tagtgaaacg tattcccggt gttacggtga tcgacaatcg ttttatcatg gtgcgcggac
     1201 tcaacgaacg ttacaacaac gtatggctga acgacgccgg tgcaccgagt tcggaaacgg
     1261 acaagcgtgc attttcattc gacatggtgc cgagcgggac gctcgaccgg atcatggtgt
     1321 acaaaacacc gtcgccggat cttcccggtg attttgcagg cggaatggtg cgcatttata
     1381 cgaaagcatt ccccgaaaaa acgatcctga atttttcttt cgagtcgaat taccgcgcag
     1441 gaacgacatt cagggatttc aattacaccg acggaacatc gaaagatatt ttcgggatgg
     1501 gcgcagcgat gcggcacatt cccgacggac ttcccgcgta catttcgcgc aacgatccga
     1561 attacacgca gatctcgcag gcattcccga acaactgggc gctgcataca cgccaggcgc
     1621 ttcccgatat gcgtttcagc atgatgtggg cgcagccgtt cacgatggga aaaaagatca
     1681 cggcgggcaa tacgctcggt ctcgggtact cgagaacgct taccacgttc agcattcgcc
     1741 ggcaggactg ggattccacc gcacagatct ccgattacac ggatattcag tgtaccgacc
     1801 aggttcgcgt cagcgccgtg gaaaattttt ccatgctcat cgggcggcag aaagtggagt
     1861 tcaaaaacct gctgaaccag atcgggaaat cgtattcgac ctaccgttac agcaattacg
     1921 cggatggccc gaatgaaaaa tcgtatgtct tcggttacga atcgcgcacg atctattcgt
     1981 cgcagctggc cggaacgttc aaaggaccga aagagcgatc ggtgtattca ttcacgggag
     2041 gttattcgta tttgctgaaa gacgcgccgg accttcgccg cattgcatat accaagcagc
     2101 agaatgcgcc cgattcaatg tacaaagccg gcatctcgac gatcgtcgac ccggtaaatg
     2161 gcggaggacg cctttatcaa tggctgcagg agcagacgtg gtcgctgcag cagaatttcc
     2221 tgcagaaagt aaacctcttc gacacgacca tagagatcaa catcggcacg tacagcgaat
     2281 acaagacaag gcaattcata gcacgcgtgc tcggttacac gatcaacccg ggcttcacgg
     2341 cgttcaacct gacgcgcgaa ccgcttgaaa cgatcttttc gccggacaac gtgggaggat
     2401 tcagtacgtt ccgcgtcgac gagatcacga acccttcgga taaatacact gcgcagaatc
     2461 tcctgctcgc cggttacgga atggcgaaaa ttcccatcgg aaaacacctc gcggttatcg
     2521 gcggactgcg ttacgaatac aacgaacagt cgctgcaggg ttacgtcaac ctcgacagca
     2581 tcagtccgaa agtgatcacg cgtttttacc tgccgtcggt gaacatgacg tataacttca
     2641 accgaaagac actcatccgc gcagcgtacg gaaaaacggt gaaccgtccc gagttccgtg
     2701 aatggtcgcc gttctatttt tacgatttcg aattcaaggc gggaacgtac ggatcgcttt
     2761 tcccgaccgt ggttgcacag aaaggacagc tgctcgatgt ggcacgtgtc gacaactacg
     2821 atctgcgctt tgaattttat cccaatgccg gtgattatat ccacgtcggc gtcttctaca
     2881 aatcattcgt ggaaccgatc cagcagatca tcctgaattc gggcggagcg gacagccgcg
     2941 cgttcacgtt cgtcaacgcg gattatgcat tcgtggaagg cgcggagctg gacatgcgga
     3001 agaacctcgc cgtgttcgac acgctttttc atacgtcggt cttcggaaac ttcaacacga
     3061 tcctcaacgt ttccgtcatg aagagccgcc tttacctcga tccgtcgcag gtggtcaacc
     3121 agtcggcggc cacgccgctg cagggacaat cgccgtacgt ggtgaatgca ggcatctatt
     3181 accagaacga ttcgatcggg ctgcaggctt cgctgctgta caacgttttc ggtccgcgcc
     3241 tttatctcgt gggcacgctg gattatgcaa acatcggcga gctgccgcgc aattcattcg
     3301 acttcgtggt aacgcagcgg atcacgccgt ggctttccgt ttccggtggt gtgcaggaca
     3361 tcttcaacca gccggtgcag atggtgcagg atacggaccg caacgggaaa ttcgagcgca
     3421 acgggcagga caaggaaata ctcaactacc gccgcggaag atattacacg atcgggatca
     3481 cgttttcacc gcaacttaac aaaggcggga aatagttttc aaatccttaa cgaggcaaaa
     3541 aggattccat aaaagcgcag gtctaagttt gctcccgtct tccgcccggt aatacaccaa
     3601 ggaatgcggg agcagttttt taaacctttc accaatcaat tcaacaacat gaaacaaaga
     3661 tccacaaaag caatcacaaa aatgtttgct gtcgtggtgg gaatgttcgc agctgccgga
     3721 gctgtcaatg cacaatctgt gatcaatgtg caggacagta tctacacgaa cacccactgg
     3781 actgcctgta atcagtacct gctgcatggt tacgtttacg taactgccgg aactacgctc
     3841 accattgatc ccggaacaat catcaaagga gacaagaaca ccaaaggaac gcttatcatc
     3901 gagcgtggtg caatgatcaa cgcgcaggga actgcaacgc agccgatcat tttcacatcg
     3961 aaccagcctg cgggtcagcg tacctacggt gactggggcg gtgtcattct tgtcggacgc
     4021 gctccgaaca actggctttc aggtcagaac caggtggaag gtggtccgcg ttccctttac
     4081 ggcggaaaca acatccacga caactccggc gtgatgagct acgtgcgcat cgaattcccg
     4141 ggtgtcgctt tttcaccgaa caacgaggtg aacggacttt ccatttattc tgtcggtgat
     4201 gcgacgcaaa ttgatcacat ccaggtttct tattccggtg acgactctta cgagtggttc
     4261 ggcggaacgg taaacgcgaa gtatctcgtt tcttatcgcc cctgggacga cagcttcgac
     4321 caggactgcg gctggagagg acacgttcag ttcgcattcg acatccgcga tccttatgcc
     4381 gctgacgttt ccggttccca tgcaatggaa atggacagct accagtcagg aacgaaatca
     4441 ggtcttgcag gcgacacttc cggtcttacc atgccggtct ggtcgaacgt gacgctggtt
     4501 ggtccgatgg tcagcccgac gactacttct tacgatcccc agtacgtttc cggaattcac
     4561 tcccgccgcg gaaacgcgac gagtgtgctg aacagcgtca tcatcggtta tccctgcggt
     4621 atccttttcg atgaatcatc cgcttcttac ggttctacca cgcgtaacct ccgcatcctc
     4681 ggctctgcag gtgattccat catgcagtac cgtggtgatg tcgtttgcgg aattcccgct
     4741 aacggttcac cggtgaaaaa agaactcgaa tacgttatcg acggagcgcg ttcactcacc
     4801 ccgacgaaca cagaaggcga cacgacaacc ggcgctcctt tcggaacatg gtctggtccg
     4861 tacaacttct acctgacccc ggcttacaag aataagatct accctaccga gcagaccggt
     4921 atttcacttc acagcccgtt caacctcgcc agccctgatc cgacgccgac cagcgtttcc
     4981 ccgatctgct acaactccac atcacttccg ggatatgtaa cagctcacta tatggcttgc
     5041 tgctctcagt cggaccattt cggtaacggc aataaatacc cgttcaaccc gagcctgccg
     5101 ctcaacacgg atacatcgaa cttcttcgcg aactacaacg cgccttctat cgttccccag
     5161 tggaacagcg gccgcctcgc cggagggttc ttcacacccg ttaactatgt cggagctttc
     5221 gcaggaacac agtctacaac tgacaactgg atgaacggat ggtgcaactt cgattgcgaa
     5281 aacaccgact acagccttac gtcagctcct gcagcttcca tcacggctct cggcaacctc
     5341 accatctgcc cgagcggcgg atcagtaaca ctgcaggcta acgcaggttc aggtttcacc
     5401 taccagtgga tgatgaacag cgccaacatt tccggtgcaa cttcacagca gtacacggcg
     5461 acaaccggcg gaacgtacca ggtaacggta acgaatgcaa ccggctgctc aacgacgtct
     5521 tcttctgttg tcgtgaacac cttcgctgct ccgtccgctt ccatcacggc aggtggtccg
     5581 accacgttct gcgcaggcaa caacgtaacg cttacagcat ccggagcatc ttcctacagc
     5641 tggagcaacg gcgccaccac gccttcgatc actgcgaaca cctccggtaa ctacctcgtc
     5701 acgatcaccg acgccaacgg ttgccaggca acgtcaagcg cagtgaacgt agtggtgaac
     5761 ccgcttccga acgcaagcgt aacggctaac ggcccgacga ccttctgcac gggcgacagc
     5821 gttgttctta ccgctaacag ctcttcttcg tatatgtgga gcgataactc gaccacgcag
     5881 agcatcacgg tttacaactc cggcaactat tcagtaatgg ttaccgatgc gaacggatgc
     5941 cagtcggctt cttccacccc gatcaccgtt tcttccagcc cgtctccgca gccgacgatc
     6001 accatcggcg gaaacacgtc actgtgccag ggccagaccg ttatgctcac ttcttctacg
     6061 gctgatagct acctctggag caatggtgca actacgcaaa gcatcacggt aagcgcttcc
     6121 ggaaactaca cggtaaccgt taccaatgcg aatgcatgca gcggaacggg agcatccaac
     6181 ccggttgcag ttacggttaa cccgaacccg acggcgaact tcaccacggg tggcaacatc
     6241 ccgaccgtga acttcacgaa cacgtcgaca ggtgctactt cctacatgtg ggatttcggc
     6301 gatttcagca gctcatcact gtcgagcccg agccacacct acggaaccaa cggcaactat
     6361 accgcatgtc tcgtcgcaat gagcgctgca ggttgcagcg ataccacctg catgccggtc
     6421 ctcatcaacg tgggcatgtc acagccgatc ctcgtcagcg acaactataa cctgtacccg
     6481 aatcctgcca acagccagct gaacatcgct gcgaacctca cgcaggaccg cgatctccag
     6541 gttgtagcgt tcgacgtgaa cggtaagctg ctcattaacg aaaaccgctc actccccgca
     6601 ggaaacaacg ttctcacgta tgacgtttcc aactgggata acgggatcta tttcatccgc
     6661 gtgatggaag gacagaatgt gcagacgatg aaagtgatca tcaaccgcta agcggtacac
     6721 atttaagaac cagtagtatg aaagggctgc ttccgcaagg gagtggccct tttgaatttc
     6781 atcttaaacc gggttctcgt caaaatcacc gggcgcgcaa gacgttttca gttcccggtt
     6841 tggagtttcg ggtttgtcca aacctgaaca gcgttgatcg tttcctgttt tacttcgtgc
     6901 acttcgtgaa aacgctccgt gtacttagtg gttttaactt tccagctttc caaccttgag
     6961 cttgaattgc tcctgtttta cttcgtgcac ttcgtgaaaa cgctctgcgt acttcgtggt
     7021 tttaactttc caacgttgaa cttgaattgc tcctgtttta cttcgtgcac ttcgtgaaaa
     7081 cgctccgtgt acttcgtggt tttacctttc caactttgaa ctcgaattgt tcccgtctcc
     7141 ctccgtgtgc tttgtgaaaa cgctccgtgt acttcgtggt tttacccttc caaccttgaa
     7201 cctaagtagt taaaatggtt tttagtttca gtgtttcagg tttgtccaat gtcattgcct
     7261 ggaatacgct gaccgttcct gttcaatgtt ctccgcataa aaaacaattc acgcaaagac
     7321 gcaaaaaaaa caccggtaca aaattgcatt gcccggtgtt gccagtgaga agaaaaaaga
     7381 aaactattcc ccgaaggcga cgatgaaata atcctgcgac cggatcattt cctttccttt
     7441 cccggccgtc accgcgatgc gggcaaccgt gcacgtattg tccacccacg aggaaacagg
     7501 gatgttccgc tgcgcgcaga tctcggtctt ttccttgtcc gaaggaagat gccgcccgac
     7561 gttccagttt tgttcggtca gcgaaaacca ataataaacg ccgttctttt cgggcgaata
     7621 atatttccct gaaaaaacgg tcttcccgtc gaagttaaaa tcatcgacaa aaaaatgaat
     7681 atccgaagtg tctttccggc cttcaaaaaa accggccacg agcaggtcgg aatgcggatt
     7741 ccattcggca cccattggtc cggcgccgtt gtgcaggaaa acatttcctg cgtgcggaag
     7801 accatccgtc ttcatttttg aaatgaaatc atccacccac ttctccgccg ttttttccgg
     7861 cggcgcatcg tgactgctgt tgcccataaa gtagtagcag gcgatctgcg tggccggctg
     7921 tcctgctgca gaatcatttt tcacggcagg aggatcagcc ggcatctggg gttgcggctg
     7981 tgcctgcgtg tcgagtatcg ctttggtgac ggaatgatcc gtttgctgtt ccgcgttatt
     8041 tccgctgcac gcagccatca ttcccgcgag cacgaatacg ggcaggtagt gtttcttcat
     8101 tggttaaaac taagaattga tcgtaaatgc tttcatcgcg gaaactaaac aacgtcgaag
     8161 ctgtaaccga tccctttgac ggttttgatg aattcctcgc cgatcttttc acgcagcttg
     8221 cgcacatgca cgtcgatcgt cctgttcttt tcaccgaact cgccgcccca gatctcgctg
     8281 tagatctcgt tgcgcgtaaa aactttccgg ggcgaagaaa gcatcagcgc gagcagttcg
     8341 aactctttcc gcgccagcac gatctctttc tgtcctttga acacgaggta acgttcgcgg
     8401 tcgaggcgga tgggcccggc ctgtgccgtt ttcggaagct cgcgcaggcg gaaccggcgc
     8461 agcagcgcgg ccacgcgcgc caccagcacg ccgggtttca tcggtttgat gatgtaatcg
     8521 tccgcaccgg ctgaaaatcc tgcgatctgt gaatagtctt cgctccttgc cgtgtaaaaa
     8581 acgatcagcg ttttgctgag cacgggtatc tgccgtatct cctggcacag ctcgatgccg
     8641 tcggtgccgg gcatgatcac gtcgagaatg ataagatgcg gctgtatgcg cgtagccagc
     8701 tctatggctt cccgcgtatt atcggaacac cagacgttat acccggcatt ttcgagaatg
     8761 cttttcaggg aaagaaggat gtgctcgtcg tcatcgacaa ggagcacccg gtaggaatct
     8821 ttacttccgt tgagcgtagt tgctgacacg aaacgaaagt atccgctcca tgttgccacg
     8881 ctgttaatcc ccggttaagt tatcttttac aaaagatgaa tgtaaaccgg ccgccattta
     8941 tgcggtgatg gccgctgcct gcaaaaacct ggaaaagagc ggatatgcat aaccatggag
     9001 gacgccggtc gcggaagaca ggtgtctttt ttacaccatt gtgtttataa taaagaagcg
     9061 ggcgcggggg aaaagcctcc cggaatgcct aatttcagta cgacgtatga aaggaaaacc
     9121 ggaatctgat tttttttaca tggtgtacga ggtggtccgc ctcgttcctt tcggccgtgt
     9181 caccacttac ggcgccatcg ccaaatacct cggcatgaaa ggttcctcgc gcatggtggg
     9241 ctgggccatg aacagctcgc acagccatca ttctcccgtt cccgcgcacc gcgtggtaaa
     9301 ccgcgaagga atgctgacgg gaaagaatca tttctccacg cctcaccgca tgcaggaatt
     9361 gctcgagaaa gaaggaataa aagtggtgaa ggaccaggtg cagaatttca aaaaccatta
     9421 ctgggatccg tcggaggagc tgaagctgta agcggaatta attgtcgttg cggtaaatgt
     9481 ccatcctgaa atccgcggcg aactgccgcg gcacgccgta atcgcaatgg aattcatcct
     9541 ttgcctgtgt ttttcgggcg gtgttctcga accccggctt gtgcgtggga aaatcgtagc
     9601 ggtcggtata gccgaaatgt ttgatgagcg tccatttccc gcctgttttt tgcaggaaag
     9661 cataacccac ctggttgatg atgcggtcgc cgcgcttgat gcggcggatg tcgaccagga
     9721 tcgccaccgg cacgccgccg ggttttccca cttccaccgt atcgcttttg gaggtgatgt
     9781 tcatcacgaa cgtttttcct ttcgggtaaa cgacgctgtc gctcccgagg aagagcttgt
     9841 cgatcgtcag gtgaacgagg tagcttttct gcgcctgcac cgaaatgcaa accgcgaaaa
     9901 gcacggcggc caggaattgt ttcatactaa aaatcaatac agcaaaacgc gtgctgaaac
     9961 gggatgttgc cctaactttg ctgcaaatat cactcaaaat gatcaccaca gaacaaatac
    10021 tggatgcgtt gcggaacgtg gacgatcccg acctgaagaa agacattgtc acgcttggca
    10081 tggtgaagga cattcacgtc gacgggaaaa aggtggcgtt caccgttgtc ctcacgacgc
    10141 ctgcgtgccc gctcaaagac atgatccgcc gcgcgtgcga gaatgccgtt aagtattacg
    10201 tcgataaaga agcggaagtg ctggtgaaca tgacagcgca cgtaacgacg aaacgaaaag
    10261 aaccttccat gcccggcgtg aagcatatca tcgcggttgc ctccgggaaa ggtggtgtgg
    10321 gaaaatcgac gatcgcggca aacctcgcct gcgcgctcgc catgcagggc gcgagcgtgg
    10381 gactcgtcga cgcggatatt tacggtccgt ccatcccgat catgttcgac gtggtgaatg
    10441 aaaaaccccg cattcagaac atcgacggca ggaaccgcat gatccccgtg gaaagctacg
    10501 gcgtgaagat cctttccatc ggttttttcg ccgacgccaa ccaggcgatc gtctggcgcg
    10561 gaccgatggc cgtgaaagcg ctcaaccaga tgctcaacga cgccgaatgg ggcgagctcg
    10621 attacatgat catcgatctt ccgcccggca ccggcgatat tcatctttcc attgtcggcg
    10681 cggttcccat caccggcgcc atcgtggtga gcacgccgca gaacgtggcg ctggccgatg
    10741 cgcgcaaggg cgtcgctatg ttccgcatgg agcagatcca cgttccggtt ctcggcatcg
    10801 tcgagaacat ggcctggttc acaccggccg aattgccgga gaacaaatac tatattttcg
    10861 ggagagaagg cgctacgcgt cttgcggaag acctgcaggt tccgctgctc gggcagattc
    10921 cgctcgtgca gagcatctgc gaagccggtg acgccggccg cccggccgtt ctccagaaag
    10981 acacgcccca ggcgaaagcc attctcgaaa tggccggcaa cgtggtacgc cgcgtggcgg
    11041 taatggatgc ggcaaaggcc gaggtcatcg cctgaaccgg aatttcgtat ctttgtgcac
    11101 tgatttttca ccatgaacgc agcattgaca gaacgcgtgg aagcagccct caaccagctt
    11161 cgtccttttc tcgaagccga cgggggaggc gtaacgcttc tcgagatcac cgccgataac
    11221 gtggtgaagc tcgaactgca gggagcctgc agatcctgtt cgatgcggat gatgacgttc
    11281 aaagccgggc tcgaagaagc ggtcaagcgc gcggcgcccg aaatcgtcgc ggtggaagcc
    11341 atcaaccttg ttgacgatgg ccagcccgtg tgacgaggat cagtgaaaac ccgttttcaa
    11401 actgtttaat tttgcaaaac acaaaccatc ccatgaaata tatgcgttca atgaactgct
    11461 gttgtatgat gcgcgcggcg catacatggg agtgactttt atgtaaccta tacagaaagg
    11521 tttttcccgc accggaaaaa ccttttttgt ttttggtcat tcaatgctga aaaaaatatg
    11581 tcgacaggaa cacaggaaat aaaaaaagaa gcggtcatcc tttttgccgg cgactcaggc
    11641 gacggcatcc agctgacagg ctcgcaattc accggaacgg cggcgtttta cggaaatgac
    11701 gtcagcacgt tcccgaactt tcccgctgaa atacgcgccc cgcaggggac gctggcaggc
    11761 gtttcgggat tccagcttca tttcggaagc atggagatca attcgcccgg cgatgcatgc
    11821 gacgtgctcg tggtgatgaa cgctgctgcg ctgaaagcaa atctcaggca gctgaaaaag
    11881 cacggcacga tcatcgcgaa caccgatggc ttcgatccga aaaatctcaa gctcgcgaaa
    11941 tatgccgatg gcgacaaccc gctgaagaac ggatcgcttt cggattatca cgtgatcggc
    12001 atcgacgtga cgaagctcac gcgcaatgcg ctttccggat ccggtctcgg cacgaaagaa
    12061 acggaccgtt ccaagaacat gttcgtgctc ggtttcatct actggatgta caaccgcaag
    12121 ccggatctca ccgttgaatt cctgaaagag caattcaaaa aacgcccaga gctcatcgac
    12181 gcgaacgtga aagtgctcat ggccggttac cattacggcg atacgacgga aacgccgatg
    12241 acgcggtatg aaataaaagc ggccaacctt cctcccggaa cctaccgcag catcacgggc
    12301 aaccagggcg
//
LOCUS       DASTSF010000552         3345 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_493667,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000552 DASTSF010000000
VERSION     DASTSF010000552.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3345
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_493667"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..119)
                     /locus_tag="VFU15_15730"
     CDS             complement(<1..119)
                     /locus_tag="VFU15_15730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005841209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HEU4719295.1"
                     /translation="MITVIPAIDIIDGKCVRLSGGDFSKTKIYRDDPVATAKE"
     gene            complement(116..703)
                     /gene="hisH"
                     /locus_tag="VFU15_15735"
     CDS             complement(116..703)
                     /gene="hisH"
                     /locus_tag="VFU15_15735"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008258591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HEU4719296.1"
                     /translation="MKIAIIDYNSGNTQSVICAMDRIGIHAFVTDDHDDIRAADKVIF
                     PGVGEAASAMKAVKEKKLDTLVLSLKQPVLGICLGMQLMCRHSEEGNTDCLGIFETQV
                     KKIPAKDDLKIPHMGWNTISGLKGPLYKGVPGNVHVYFVHSYCADLCAETNAVTDYSV
                     RFSAGLSKNNFHGVQFHPEKSGVAGEFILRNFISL"
     gene            complement(700..1716)
                     /locus_tag="VFU15_15740"
     CDS             complement(700..1716)
                     /locus_tag="VFU15_15740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018478039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-deoxy-7-phosphoheptulonate
                     synthase/chorismate mutase"
                     /protein_id="HEU4719297.1"
                     /translation="MIIRLHKTISPADETVVRAKIGSAGYKETEVVTRQNRYLVCTGT
                     KPLDIRAFGNLPGVSDVHFVSDVNQLVSREWKVQDTIVDLGNDCRIGNGTFTVMAGPC
                     SVESEEQVRKTVGFLIENNIRIMRGGVFKPRSSPYAFRGLGIDGLKMFHDICSEKGIR
                     IVTEVMQADQIEDMFPYVDIFQVGARNAQNFTLLDALGRCGKPVLLKRGLSGTIEELL
                     ASAEYIFSNGNEQIILCERGIRTYEKAYRNTLDLNAVPILKSRSHLPVIVDPSHGIGI
                     RSHVEPVALAAVMAGADGIIAEIHETPEKALSDGQQTLNFEEAAALTARLRKTYGLRK
                     SFGK"
     gene            complement(1697..2494)
                     /gene="trpA"
                     /locus_tag="VFU15_15745"
                     /pseudo
     CDS             complement(1697..2494)
                     /gene="trpA"
                     /locus_tag="VFU15_15745"
                     /inference="COORDINATES: protein motif:HMM:NF012511.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
     gene            complement(2481..>3345)
                     /gene="trpB"
                     /locus_tag="VFU15_15750"
     CDS             complement(2481..>3345)
                     /gene="trpB"
                     /locus_tag="VFU15_15750"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEU4719298.1"
                     /translation="AETGAGQHGVATATVCALAGLRCIVYMGETDMKRQAPNVARMKM
                     LGAEVRGVTSGSKTLKDATNEAIRDWISHPADTHYIIGSVVGPHPYPDIVARFQSVIS
                     REIKKQLEEKTGNENPDCVIACVGGGSNAAGAFWHFLGDENVKLIAVEAAGKGIHSGL
                     SAATSVLGRPGVIHGSRTLLMQDGDGQITEPHSISAGLDYPGVGPLHAHLHGTGRAEF
                     LSATDDEALQAAYELAKLEGIIPALESAHALAALSKKKFARNEIVVVNLSGRGDKDLE
                     TYINHLPHEQD"
BASE COUNT          685 a          947 c          871 g          842 t
ORIGIN      
        1 aattcttttg ccgtcgctac ggggtcgtcg cggtagatct tcgtttttga aaaatcgccg
       61 ccgctgaggc gcacgcattt cccgtcaatg atatcgatgg ccggaataac tgtgatcata
      121 ggctgataaa attccgaagt atgaactctc ctgcaacacc gcttttttcg gggtggaact
      181 gcacgccgtg gaaattattt tttgacaggc ccgccgaaaa cctcacgctg taatccgtta
      241 ccgcattcgt ttccgcacaa agatccgcgc agtacgaatg aacgaaatac acgtgcacat
      301 ttccgggaac gcctttgtac agcggacctt tcagcccgct gatcgtgttc catcccatgt
      361 gcgggatttt cagatcgtct tttgcgggaa tttttttcac ctgtgtttcg aagatgccga
      421 gacaatccgt gttgccttct tcggaatgcc tgcacatgag ctgcatgccg agacagatgc
      481 ccagcaccgg ctgttttaac gagagaacaa gtgtatcgag ttttttctct ttcactgctt
      541 tcatggcaga cgcagcttca cctacaccgg gaaagatcac tttatcagcc gcccggatat
      601 catcgtgatc atcggtcacg aacgcgtgaa taccgatgcg gtccatggcg cagatcaccg
      661 actgcgtatt ccctgaatta taatcgatga tggcgatttt cattttccga aagactttcg
      721 cagcccgtac gtttttctca gtcttgctgt gagcgccgcc gcttcttcaa agttaagcgt
      781 ttgctgaccg tcggaaagcg ctttttccgg tgtttcatgg atttctgcaa tgatgccgtc
      841 tgcgcctgcc atcacggccg ccaatgccac aggctcgacg tgtgaacgta taccgatgcc
      901 gtgcgaagga tcgacgatca cgggaaggtg cgatcgcgat ttcagtatcg gaaccgcgtt
      961 caggtcgagt gtgttccggt acgccttttc gtaggtgcgg ataccgcgtt cgcagagaat
     1021 gatctgctcg ttgccattgg aaaaaatata ttccgctgaa gcgagcagct cttcgatcgt
     1081 gccggaaagt ccgcgcttga gcaacaccgg ctttccgcac cggcccagcg cgtcaagcag
     1141 cgtaaagttc tgcgcattcc gtgcacccac ctggaaaatg tccacataag ggaacatgtc
     1201 ttcgatctgg tcggcctgca tcacctcggt aactatacgg atcccttttt cggaacagat
     1261 atcatgaaac attttcagcc cgtcgatgcc gagcccgcga aacgcatagg gagaactcct
     1321 tggtttgaaa acgccgcccc gcatgatgcg gatgttgttt tcaatgagaa aaccgacggt
     1381 cttccgcacc tgctcttcgc tctcgacgga gcagggtccg gccatcacgg taaacgttcc
     1441 gttcccgatg cggcaatcgt tcccgagatc cacgatcgtg tcctgcactt tccattcgcg
     1501 ggaaaccagc tggttcacat ccgaaacaaa atgcacgtcg ctcacgcccg gcaggttccc
     1561 gaacgcccgt atatcgagcg gttttgttcc tgtgcagacg aggtaacggt tttgccgggt
     1621 gacgacttcc gtttctttgt agccggcgct gccgatcttt gcgcgcacaa cggtttcgtc
     1681 ggcgggagaa atagttttat gcaaccggat gatcatggtg ttttgttttt atgcgctgaa
     1741 taaaatgtgc tgcgccgctg cggatgtcct ttgcttccgc gagtgcgcgg atgaacgcgc
     1801 ttcccacgac ggcgccgttc gtgtgcgctg aagctgctct gaacgtttcg tgatcatgaa
     1861 ttccgaaacc ggtcagcacc gggatttcaa aaccacggcc ggtcgctttt ttcaatgctt
     1921 actgctgcgc cgggctgaaa tttttgtctg caccggtcgt tgatgccgct gaaaccaggt
     1981 acaaaaatcc tttgctcagc ttcgcgatct gcgccctcct gttttccggc gtacgcggtg
     2041 tcacaaggaa aatgaaatgg aggtggcttt gttcgaagat cgtcctgaat tccctttcgt
     2101 agatttccgg cggaagatca gggaggatca ccccgtcgat cccggctgct gcgcattcct
     2161 caacaaattt tttcacgccg aactgcagga caggattgac atatcccatc agcacgacag
     2221 gcagtgaaat tttttgccgg aaatttttca gctggcggaa caggagcgaa atgctcatcc
     2281 cgttttccag cgccacgcgc gagctttcct ggatcaccgg tccatcggca agcggatcgg
     2341 agaacggcat gccgatctcg acaagatccg ctcctgcttc ctggagcgct tccagcacgg
     2401 gaagcgtatc gttcagcttc gggaaaccgg ccgtaaagta aacggcgaga acttcttttt
     2461 tctttttggc gaatagcgaa tcaatcctgt tcatggggaa gatggttgat gtacgtttca
     2521 agatccttat caccgcgacc ggaaagattc acaacgacga tctcgttacg cgcgaatttt
     2581 ttcttcgaca atgccgcgag cgcatgtgca ctttccagcg cgggaatgat gccttcgagc
     2641 tttgccagct cgtacgccgc ctgcagcgct tcgtcatctg ttgcagacag gaattccgcg
     2701 cgacccgttc cgtgcaggtg cgcatgcagc gggccgacac cgggataatc gagaccggca
     2761 gagatcgaat gcggttcggt gatctgcccg tctccgtcct gcatgagcag cgtccggctt
     2821 ccgtggataa cacccggtct tcccagcacg gaagttgccg cggaaagtcc ggaatgaatt
     2881 ccttttccgg ctgcttccac ggcgatgagc ttcacattct cgtcaccgag aaaatgccag
     2941 aacgcacctg ctgcattgct gccgccaccg acgcacgcga tcacgcagtc tgggttttca
     3001 ttcccggttt tttcttccag ctgctttttt atttcgcggc tgatcaccga ctggaaacgt
     3061 gcgacgatgt cgggatacgg atgcggtccc acgacagagc caatgatgta atgcgtatcc
     3121 gccgggtgac tgatccagtc gcgaatggct tcgttggttg catctttcag cgttttgctt
     3181 ccggatgtta caccacgcac ttccgctccc agcattttca tccgcgcgac gttcggggcc
     3241 tggcgtttca tatctgtctc gcccatgtac acgatgcagc gtaatcccgc gagtgcgcac
     3301 accgtcgcag tggcgactcc gtgctgtccc gccccggttt ccgca
//
LOCUS       DASTSF010000553         2062 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1445701,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000553 DASTSF010000000
VERSION     DASTSF010000553.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1445701"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..733)
                     /locus_tag="VFU15_15755"
     CDS             complement(<1..733)
                     /locus_tag="VFU15_15755"
                     /inference="COORDINATES: protein motif:HMM:NF012981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEU4719299.1"
                     /translation="MNRRKVAFVINPNSGSDRKTDRVKLVESLRSDSYESEILLWKKI
                     EDKDDIFSRARNGGFDIAVAVGGDGTVSQLAETLCGTNVALGIVPFGSGNGLARHLGV
                     PLDPAGAMKLIETGKIEKIDRGKFGERSFFCTAGVGFDARIGKLFAESTTRGFWTYAK
                     MTLREFRSYAPEKYSITVDGKKTETEAFLVTVANAGQYGNDAWIAPKAKINDGLLHLS
                     VLRPFRWWNMPGIALKFFRKKIDRSP"
     gene            complement(768..1907)
                     /locus_tag="VFU15_15760"
     CDS             complement(768..1907)
                     /locus_tag="VFU15_15760"
                     /inference="COORDINATES: protein motif:HMM:NF024918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HEU4719300.1"
                     /translation="MKTKVIFAVQGEGRGHMTQALSLKQLLDRNGFEVCCVLVGSSER
                     REIPAFFQEKFPGTEVIRVKSPNFILKNNRGIRIGKSVMRNLWKLRTYLRSARLLKEK
                     VKEHAPQLIINFYEPVVALYCLTNRRTHPPVVSIAHQYLGEHPEFKFPDGYMMDKYFL
                     KNYTAFTAFGSSRILALSFYPLDDVKNQKLKVVPPLLRREAKEIQPTQEDFLLCYLVN
                     AGYRSDIESWHAKNPSQELHVFTDMKSDKQVIEVKPKLFFHKLNDVEFLRHMAACKGL
                     ISSAGFESICEAMYLGKPVLMVPVEGHFEQRCNSRDGFRAGAGIYDDRFDITRFLNWL
                     SERKPGNGDFRTWESKAEELFLRNFREVLNKAQKEIQVEGSLASA"
     gene            complement(1904..>2062)
                     /locus_tag="VFU15_15765"
     CDS             complement(1904..>2062)
                     /locus_tag="VFU15_15765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005868472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HEU4719301.1"
                     /translation="HYLNSGDWVESMTALVETEKGEWKIIEYAGWLAGQSAADTVSET
                     PTIKLASA"
BASE COUNT          414 a          572 c          575 g          501 t
ORIGIN      
        1 aaggcgaccg gtcgatcttt ttcctgaaaa atttcagcgc gatcccgggc atgttccacc
       61 agcggaaagg cctcaggacg gaaagatgca gcagcccgtc gttgatcttc gccttcggtg
      121 cgatccacgc atcgttgccg tactggcccg cgttcgctac agtgaccagg aacgcttcgg
      181 tctccgtttt tttcccgtcg acggtgatgg aatatttttc cggtgcatac gacctgaatt
      241 cacgcagtgt catttttgcg tacgtccaga aaccgcgcgt cgtgctttcc gcgaaaagtt
      301 tcccgatgcg cgcgtcgaac ccgacgcccg cggtacagaa gaaagaacgt tccccgaatt
      361 tcccgcgatc gatcttttcg atcttcccgg tttcgatcag cttcatcgca cctgcaggat
      421 cgagcggaac accgagatgg cgcgcgaggc cgttgcccga cccgaacgga acaattccca
      481 gcgcgacgtt cgtgccgcag agcgtttccg cgagctggct cacggtaccg tctccgccca
      541 ccgcgacggc gatgtcgaaa cctccgttgc gcgcgcggct gaagatatcg tccttgtctt
      601 cgatcttttt ccagaggaga atttcacttt cgtagctgtc gcttcggaga gattccacga
      661 gcttaacacg atctgttttg cgatcacttc ccgaattggg attgatcacg aacgcgactt
      721 ttctgcgatt catgccggca aagtaactaa aaaacggggg tgtgaaatca cgcggatgcg
      781 aggctgcctt ctacctggat ttctttttgc gccttgttca gcacttcgcg gaaattgcgc
      841 aggaacagtt cttccgcctt gctttcccac gtgcgaaaat cgccgttgcc gggtttccgt
      901 tccgaaagcc agttgaggaa acgcgtgatg tcgaacctgt cgtcataaat tcctgcgccc
      961 gcacggaacc cgtcgcgtga attgcagcgc tgctcgaaat gtccttccac gggaaccatg
     1021 agcacgggct tgccgaggta catcgcttcg cagatcgact cgaaaccggc cgagctgatc
     1081 agtcctttgc aggcggccat gtggcggagg aattccacgt cgttcagctt gtgaaagaaa
     1141 agcttcggct tcacttcgat cacctgcttg tcgcttttca tgtcggtaaa aacgtgcagc
     1201 tcctgcgaag gattctttgc atgccagctt tcaatgtcgg agcgataacc ggcattgaca
     1261 agatagcaga gcaggaaatc ttcctgcgtg ggctgaatct ctttggcttc gcggcggaga
     1321 aggggaggaa cgaccttcag cttctggttt ttcacgtcat ccagagggta aaaagaaagc
     1381 gcgagaatgc gcgacgaacc gaaagcggtg aatgcggtgt agttcttcag gaagtatttg
     1441 tccatcatgt agccgtccgg gaatttgaat tccgggtgtt cgccgaggta ctggtgcgcg
     1501 atgctgacca cgggcggatg cgtacgcctg ttcgtgaggc agtaaagggc aacgaccggc
     1561 tcgtagaaat taatgatgag ctgcggtgca tgctccttca ctttttcttt cagcaggcgg
     1621 gcgctgcgca ggtacgtgcg gagcttccac aggttgcgca tgacgctttt cccgatgcgg
     1681 atgccgcggt tgttcttcag gataaagttg ggactcttca cgcggatgac ttccgtaccc
     1741 gggaattttt cctggaaaaa agccgggatt tctcggcgtt cactcgatcc cacgagcacg
     1801 cagcagactt caaaaccgtt ccggtcgagc agctgcttca gcgagagcgc ctgcgtcatg
     1861 tgaccgcggc cttcgccctg tacggcaaaa atcacttttg ttttcatgcg gatgcgagtt
     1921 taatggtggg agtttcgctg accgtgtcag cggcggattg tccggcgagc cagccggcgt
     1981 attcgatgat cttccattcg cctttctctg tttccacgag cgcggtcatc gattccaccc
     2041 agtcgccgct gttcaggtaa tg
//
LOCUS       DASTSF010000554         4778 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_403389,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000554 DASTSF010000000
VERSION     DASTSF010000554.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4778
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_403389"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..680)
                     /locus_tag="VFU15_15770"
     CDS             complement(<1..680)
                     /locus_tag="VFU15_15770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719302.1"
                     /translation="MKAPKDSDDAYYSLKDAKKDRIAEQKRKDDEKKREEDEARQRDL
                     DQKTADARSKPGSDYYDQSFDYDDYYDYEYAARLRRFDHPVNGAGYYDNYYTNSYWYN
                     NNPYCYGTSIYYGYNFWGPTYYAYNYYPSSFFYYNSGWGWGTGYGCYNPWGGGWYDPW
                     NPYGWGYNDPYANPYYCGWGYPYYGGGWYGTGIGYPYGYGAGYGNGWNSQYSNNYFNS
                     FDSNSYYYG"
     gene            complement(655..879)
                     /locus_tag="VFU15_15775"
     CDS             complement(655..879)
                     /locus_tag="VFU15_15775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719303.1"
                     /translation="MVKTLWNDFCTFISENFAKVRIIPVSEIRETQFLKNKKTYRHET
                     FRDYQFFRPYRAFICLLPGDEIHEGAERQR"
     gene            893..2368
                     /gene="proS"
                     /locus_tag="VFU15_15780"
     CDS             893..2368
                     /gene="proS"
                     /locus_tag="VFU15_15780"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEU4719304.1"
                     /translation="MAKELTKRADDYSQWYQDLVTKADLAEHSAVRGCMVIKPYGYAI
                     WENMQATLDKMFKETGHVNAYFPLFVPKSLFEAEEKNAEGFAKECAVVTHYRLKADPS
                     KKGSLMVDPDSKLEEELIVRPTSEAIIWKTYKGWIQSYRDLPLLVNQWANVVRWEMRT
                     RLFLRTAEFLWQEGHTAHATREEAIEETEKMLGVYADFAENWLAVPVIRGLKTPNERF
                     AGADETYCIEALMQDGKALQAGTSHFLGQNFAKAFDVKFSGKDGKLEYVWATSWGVST
                     RLMGALVMAHSDDNGLVIPPKLAPVHVVIVPIYKSDEERAMITERVTVLKKELEAKGL
                     RVKYDDRDTQRPGFKFAEHELRGVPVRLGIGPKDLANNTVELARRDLMTKENVSADGL
                     ADKIQAMMEDIQSNLFNRALERRKAMTHYVDTWADFEAALDKGGFVYAHWDGTPGTEE
                     AIKDKTKATIRCIPLDNPKEDGKCILTGNPSVQRVVFARAY"
     gene            2394..3086
                     /locus_tag="VFU15_15785"
     CDS             2394..3086
                     /locus_tag="VFU15_15785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719305.1"
                     /translation="MSPKKSQELIRDLLKEKSSLKQDVFERTKQAFTQLGKVLGEIAD
                     ELRPVAAKIDKRLVVEFREKNEFEMEFRVAGDVLLFHMHTNVFEFESSHMIWKTSYVR
                     DNPSNSYCGVIHIYNFLADSFKYNRTNDLGYLIGRLFLNRENHYFVEGKRQLGFLYND
                     FTEAVLTKDDLRKIVESAIVYCLDFDLFTPPFEEVKVVTLAQVIEESSLSAMKTGKRL
                     GFRFQADTDHFE"
     gene            complement(3102..4190)
                     /locus_tag="VFU15_15790"
     CDS             complement(3102..4190)
                     /locus_tag="VFU15_15790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HEU4719306.1"
                     /translation="MEKIFSKLDRYILRKFIGTFVVSILLILFIVIVFDISEKIEDFV
                     NHKVALKEIVFDYYLNFIPYIVNLFSPLFTFIAVIFFTSRLASRTEIVAMLSSGISFN
                     RLLRPYLVGATLIAGTSLFLNNYLIPRANKTRLDFESKYVGSQYVFDGKNIHREVDKD
                     IYVYMETYNSTENVGTRFTLEKMKGLDLVFKLTAREIKWDSIRNRWSIIDYYGRYIDG
                     RKEYFVKGDRIDTTLNLTPKDFGRTDNVIKSMTAGELNRFIDDEKKKGSDSVQIYEIE
                     KHSRIAYPFATFILTLIGVSVSSRKTRGGIGMHLGIGLGLSFGFIFFMQLFTSYAQSG
                     VIPAYIAVWIPNIIFAAVAIFMLRKAPK"
     gene            complement(4203..>4778)
                     /locus_tag="VFU15_15795"
     CDS             complement(4203..>4778)
                     /locus_tag="VFU15_15795"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014083845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEU4719307.1"
                     /translation="YPQTLFPIVQGSTYRDLRQASAEFIAAQEREGNAIGGLSVGEPA
                     EVMYELTELVTSILPKEKPRYLMGVGTPQNILEGIASGVDMFDCVMPTRNARHGLLFT
                     SSGIINIHNEKWKDDHSPVDPEGITFADRHTRAYLRHLFMCGEMLGPMIASVHNIGFY
                     LWLVKEARKKIQEGNFASWKNTMIPKLGQRL"
BASE COUNT         1150 a         1185 c         1344 g         1099 t
ORIGIN      
        1 ccgtagtaat agctgttgct gtcgaagctg ttgaagtagt tgttggaata ctggctgttc
       61 cagccattgc cgtaaccggc tccgtatcca tacgggtaac caattccggt tccgtaccat
      121 ccgccgccgt aataagggta accccatccg cagtaatacg ggttggcata cggatcatta
      181 tagccccagc catacggatt ccacggatca taccagccac caccccaggg attgtagcag
      241 ccgtaaccgg ttccccatcc ccatccgctg ttgtagtaga agaaggagct cgggtaataa
      301 ttgtaagcat agtaagtcgg tccccagaaa ttatacccgt agtaaatgga cgtgccgtag
      361 cagtacgggt tgttgttgta ccaatagctg ttggtgtagt agttgtcata atagccggca
      421 ccgttcacgg gatgatcgaa gcggcggaga cgcgcggcat attcgtagtc gtagtaatcg
      481 tcgtaatcga atgactggtc gtaataatca gagcccggct tggaacgggc atcggccgtt
      541 ttctggtcga gatccctttg acgcgcttcg tcctcttcac gttttttctc gtcgtctttc
      601 cgtttttgct cggcaatgcg atcttttttg gcgtctttca gggaataata ggcatcatcg
      661 ctgtctttcg gcgccttcat ggatttcatc ccccggcagg aggcaaatga aagcgcgata
      721 agggcggaaa aactgataat cgcgaaacgt ttcatggcgg taagtttttt tgttttttaa
      781 aaactgtgtt tcgcgaattt cgctgacagg gatgattcgt acttttgcga aattctctga
      841 aataaaagta caaaaatcat tccagagtgt ctttaccact tataagaatt ttatggccaa
      901 agaactgaca aaacgagccg acgactattc ccaatggtac caggacctgg tgaccaaggc
      961 tgatcttgcc gaacattccg ccgtgcgcgg gtgcatggtg atcaagccgt atggctatgc
     1021 gatatgggaa aacatgcagg ccactctcga taaaatgttc aaggaaacgg gccacgtcaa
     1081 cgcctatttc ccgctcttcg ttccgaaaag tcttttcgag gcggaagaga agaacgccga
     1141 aggttttgcc aaggaatgtg ccgttgtgac acattaccgc ctgaaagccg atccttcgaa
     1201 gaaaggttcg cttatggtcg atccggattc gaagctggag gaagagctca tcgtgcgccc
     1261 cacaagcgaa gccatcatct ggaaaacata caaaggctgg atccagtcgt accgcgacct
     1321 gccgctgctg gtgaaccagt gggcgaacgt cgtgcgatgg gaaatgcgta cgcgcctgtt
     1381 tctccgcact gcggaattcc tctggcagga aggccatacg gcccatgcaa cacgcgaaga
     1441 ggcgatcgaa gagacggaaa agatgctcgg cgtgtatgcc gattttgcag agaactggct
     1501 cgccgttccg gttatccgcg gactgaaaac accgaacgag cgtttcgccg gtgcggatga
     1561 aacgtattgc atcgaggcgc ttatgcagga tggaaaagcg ctgcaggcag gaacgtcgca
     1621 ttttctcggg cagaatttcg ccaaagcatt cgacgtgaaa ttctcgggga aagacgggaa
     1681 gctcgaatac gtgtgggcca cctcgtgggg cgtttcgacg cgactgatgg gtgcgctcgt
     1741 catggcgcac agcgacgaca acggtcttgt cattcctccg aagctcgcgc cggttcatgt
     1801 ggtgatcgtg cccatttaca aatcggatga agaacgcgcg atgatcacgg aacgcgtgac
     1861 cgtgctgaag aaagagctgg aggcgaaagg acttcgcgtg aaatacgacg accgcgatac
     1921 gcagcgcccg ggattcaaat tcgccgagca tgaattgcgc ggtgttcccg tgcgtctcgg
     1981 aatcggtccg aaggatctcg cgaacaacac ggtcgagctg gcgcgccgcg accttatgac
     2041 aaaggaaaat gtttcagcgg acggactcgc agataaaata caggcgatga tggaagacat
     2101 ccagtcgaac ctattcaacc gcgcgctcga acgcaggaag gcgatgacgc attacgtgga
     2161 tacgtgggcg gacttcgaag cggcactcga caaaggcggg tttgtctatg cgcactggga
     2221 cggaacgccg gggacggaag aagcgatcaa ggacaagacg aaagcaacga tccgctgcat
     2281 tccgctggac aatccgaaag aggacggcaa atgcatcctg acgggaaatc cttccgtgca
     2341 gcgtgtcgtg tttgcacgtg cctattgacg aaaagaaaag taacttcgtg atcatgtcgc
     2401 cgaaaaagag ccaggaatta atacgggacc tgctgaagga aaaatcttcg ctgaagcagg
     2461 atgttttcga gcggacgaag caggcgttca cgcagctggg caaggtgctg ggggagattg
     2521 cggacgagct gaggccggtc gcggcgaaga tcgacaagcg gctggtggtg gaattccgcg
     2581 agaagaacga gttcgaaatg gaattccgcg tggccggcga cgtgcttctt tttcacatgc
     2641 acacgaacgt gttcgagttc gaatcgagcc acatgatctg gaaaacgtcc tacgtcaggg
     2701 acaatccttc gaattcatat tgcggcgtga tccacatcta taatttcctg gcggattcat
     2761 tcaagtacaa ccgcacgaac gatctcggct acctcatcgg gcgcctgttc ctgaaccgcg
     2821 aaaatcatta tttcgtggaa gggaaacgac agctcggctt cctctacaat gattttacag
     2881 aagcggtgct gacgaaagac gacctgcgga aaatcgtgga atcggctatt gtgtattgcc
     2941 tcgatttcga cctgttcaca ccgcctttcg aagaagtgaa ggtcgtaacg ctcgcgcagg
     3001 tgatcgagga aagctcgctc tctgcaatga aaacgggcaa gcgcctcggg ttccgtttcc
     3061 aggcggacac ggatcatttc gaataataat ttccgggaaa tttatttggg tgctttgcgc
     3121 agcatgaaaa tggctactgc ggcgaagatg atgttcggta tccacaccgc gatataggcg
     3181 ggtatcacgc ccgactgcgc gtaggaagtg aacaactgca tgaagaaaat gaacccgaag
     3241 ctgagcccga gcccgatgcc gagatgcatg cctatgccgc cccgtgtttt ccggctcgac
     3301 acggaaacgc cgatgagcgt aaggatgaac gtggcgaacg ggtaagcgat gcggctgtgc
     3361 ttttcgatct cgtagatctg tacgctgtcg gaaccttttt tcttttcatc gtcgatgaag
     3421 cggttcagtt ccccggcggt catgcttttg atgacgttgt cggtgcgacc gaaatctttc
     3481 ggcgtcaggt taagcgtcgt atcgatcctg tcgcctttca cgaaatattc tttccggccg
     3541 tcaatgtacc ggccgtaata atcgatgatc gaccagcggt tgcggatgct gtcccatttt
     3601 atttcccttg ccgtcagttt gaaaacgagg tccagccctt tcattttctc gagcgtgaac
     3661 cgtgtcccca cgttttccgt gctgttgtac gtttccatgt aaacataaat gtccttgtcg
     3721 acctcgcggt gaatgttctt cccgtcgaat acgtactggc tgccgacgta tttcgattcg
     3781 aaatcgagac gtgttttatt ggcgcggggg atgaggtaat tgttcaggaa aagcgacgtg
     3841 cccgcgatga gcgtggcgcc gacgaggtaa ggacgcagca ggcggttgaa ggaaataccc
     3901 gagctgagca tcgcgacgat ctcggtgcgc gaggcgagcc gcgaggtgaa aaagatgacc
     3961 gcgatgaacg tgaacagcgg gctgaacagg ttgacgatgt agggaatgaa attgaggtag
     4021 taatcgaaca cgatctcttt cagcgccacc ttgtggttga cgaaatcttc gatcttttcc
     4081 gagatgtcga atacgatgac aatgaaaaga atgagcagga tggaaacgac gaacgtcccg
     4141 atgaatttcc tcaggatgta acggtcgagc ttcgaaaata tcttttccat cggcagtgaa
     4201 gatcagaggc gctgacccag cttcgggatc atcgtgtttt tccaggaggc gaaatttcct
     4261 tcctgtatct ttttccgcgc ttctttcacc agccagaggt aaaaaccgat gttgtgcacg
     4321 gatgcgatca tcggtcccag catttccccg cacatgaaca ggtgacgcag gtatgcgcgc
     4381 gtgtgcctgt ccgcaaaagt tatgccttcc gggtcgacgg gcgaatgatc gtccttccat
     4441 ttttcattat gaatgttaat gatgccggaa gaagtgaaca gcagcccgtg acgcgcgttg
     4501 cgcgtaggca tcacgcagtc gaacatatcc acgccggacg caatgccttc gaggatattc
     4561 tgcggtgttc cgacgcccat caggtaacgc ggcttttcct tcggaagaat ggacgtgaca
     4621 agctccgtca gctcgtacat cacttcagcc ggttcgccta ctgaaagtcc gccgatggcg
     4681 ttcccttcgc gttcctgtgc agcgatgaat tcggcggaag cctgccgcag gtcgcggtac
     4741 gtgctcccct gcacaatggg aaaaagtgtt tgcgggta
//
LOCUS       DASTSF010000555         2675 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_960552,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000555 DASTSF010000000
VERSION     DASTSF010000555.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2675
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_960552"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1057
                     /locus_tag="VFU15_15800"
     CDS             <1..1057
                     /locus_tag="VFU15_15800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010231714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEU4719308.1"
                     /translation="IEEANREEKFPRHLIPKMAEMGLFGANINEYDLPGLNNVAYGLI
                     MQELERGDSGIRSTASVQSSLVMYPIWSFASEEQKKKYLPKLATGELIGCFGLTEPDF
                     GSNPAGMITNYKDKGDHVVLNGAKMWISNAPFADIAVVWAKDEAGEIHGLVVERGMEG
                     FTTPETHGKWSLRASATGELVFDNVKVPKENIFPKVKGLKGPLSCLNSARYGISWGAI
                     GAAMDCYDTALRYSKERIQFGKPIGAFQLQQKKLAEMITEITKAQLLCWRLGVLKNEN
                     RATPAQISMAKRNSVHTAITIAREARQMLGGMGITGEYPIMRHMMNLESVITYEGTHD
                     IHLLITGMDVTGFNAFN"
     gene            complement(1066..>2675)
                     /locus_tag="VFU15_15805"
     CDS             complement(1066..>2675)
                     /locus_tag="VFU15_15805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719309.1"
                     /translation="LLLIAYIAMGWFFRMVFIARKEKKNTSATISFLLKHLRYPLIAA
                     VAGYFLGMIFWPYGLTNPFTHPFIALSEFSNYDKFDSYNLFEGRWIHRWEIPWNYIPE
                     WIWITTPLFINLIFIFIPLSFFKKLTGTISICRGYLALVCFVAAFPVFYIIYRHSNVY
                     DGWRHAFFVYPSFAAACAVTWTTVIQIVKPKFYRYAIVAILAGTMIQPAAWMIRNHPF
                     ESFYFSPVIGGTKGAFKKFEIDYYGTSYRQAVEWIASHADTTGKGPARVRCYYGETES
                     SKHFVDKYKNLRYVFANENSLDWDYSIVLPCQAKLDSMLLVNWPPQGTVWQAYADSTP
                     IVAVVKNFRTKDMLPDPRTSVYSSTDVNYLLNMSLSFYNAGDYFSCIAACERALQFDP
                     GNMIAYNNKCTALNRLLLYPDALEAGAAGLAVDSSYALLKGNVKEAQQGMNAKPDPGI
                     LAANYLNLSVVYYNLGLYRKTIELCKKALRYDPQSAIAYNNICSSYNALGEYRSADSA
                     CSKGLSIDPKNALLQNNLGEAKKHEGK"
BASE COUNT          637 a          701 c          769 g          568 t
ORIGIN      
        1 catcgaagaa gcgaatcgcg aggagaagtt cccccgccac ctcatcccga agatggcgga
       61 gatgggtttg ttcggcgcaa acatcaacga gtacgacctc cctggcctga acaacgtcgc
      121 ctacggcctg atcatgcagg agctcgagcg cggcgattcc ggcatccgtt ccacggcgtc
      181 ggtgcaaagc tcgcttgtca tgtacccgat ctggtctttc gcttcggaag agcagaagaa
      241 aaaatacctg ccgaagctgg ctaccggcga gctcatcggc tgcttcgggc tgacggaacc
      301 tgatttcggt tccaacccgg cgggaatgat cacgaattat aaagacaaag gcgatcacgt
      361 cgtcctcaac ggcgcgaaga tgtggatctc gaacgctccc ttcgcggata tcgcagtggt
      421 gtgggcgaaa gacgaggcgg gagaaattca cgggctcgtc gtggaaagag gaatggaagg
      481 attcaccacg ccggaaacgc acgggaaatg gagcctgcgc gccagcgcaa cgggcgagct
      541 cgtgttcgac aacgttaagg tgccgaagga aaatattttc ccgaaagtga aaggactgaa
      601 aggtccgctg agctgtctca actccgcgcg ctacggaatt tcctggggcg ccatcggtgc
      661 ggcgatggat tgttacgaca cggcgctccg ttattcgaaa gagcgcatcc agttcggtaa
      721 gcccatcggt gcattccagc tgcagcagaa aaagctggcc gagatgatca ccgagatcac
      781 gaaagcgcag ctgctctgct ggcgtctcgg tgtgctgaag aacgagaacc gcgccacgcc
      841 cgcgcagatc tccatggcca agcgcaacag cgtgcacacc gcgatcacca ttgcgcgtga
      901 agcgcgccag atgctcggcg gtatgggcat caccggcgaa tacccgatca tgcgccacat
      961 gatgaacctc gaatcggtga tcacctacga gggcacgcac gacattcacc tgctcattac
     1021 gggaatggat gtgacgggat tcaacgcgtt caactgagaa ggaaattact ttccttcatg
     1081 cttcttcgct tcgccgaggt tattctgcaa taacgcattt ttcggatcga tggacagccc
     1141 cttgctgcat gcagagtcgg cagacctgta ttcaccgagc gcattgtagg aagagcagat
     1201 gttgttatag gcgatcgcgc tttgcggatc gtaacgcagc gcttttttac acagttcgat
     1261 cgttttgcgg tacaggccga ggttgtaata cacgacgctc aggttgaggt aatttgccgc
     1321 caggatgccc ggatccggtt tcgcattcat tccctgctgt gcttccttca catttccttt
     1381 cagcagcgca tacgacgagt cgacggcgag tcctgcggcg ccggcttcaa gcgcatccgg
     1441 ataaagcagc aggcggttca gcgcagtgca tttgttgttg tacgcgatca tgttccccgg
     1501 atcgaattgc agcgcacgtt cgcaggcggc gatgcaggaa aaataatcgc cggcgttgta
     1561 gaacgaaaga ctcatgttca ataaataatt cacgtccgtg ctgctgtaaa cggatgtccg
     1621 cgggtcgggc agcatgtctt ttgtgcggaa gtttttcacc acggcgacaa tgggcgtgct
     1681 gtcggcgtac gcctgccaga cggttccctg cggcggccag ttgaccagca gcatcgaatc
     1741 cagcttcgcc tggcacggga gcacgatcga ataatcccag tcgagggaat tttcattcgc
     1801 aaaaacatag cgcaggttct tgtatttgtc gacgaaatgt ttcgagcttt ccgtttcgcc
     1861 gtagtagcaa cgcacacgcg ccgggccttt ccccgttgta tccgcgtgcg atgcgatcca
     1921 ttcgacggcc tggcggtaag aagtgccgta ataatcgatc tcgaattttt tgaacgcacc
     1981 ttttgttccc ccgatcaccg ggctgaaata aaaactttcg aaggggtggt tgcggatcat
     2041 ccacgcggcc ggctggatca tcgttcctgc cagtatggcc acgatcgcgt agcggtaaaa
     2101 tttcggcttc acgatctgta tgacggttgt ccacgtcact gcgcaggcag cggcgaagga
     2161 agggtaaacg aaaaacgcat ggcgccatcc gtcgtaaaca ttgctgtgcc tgtaaatgat
     2221 gtaaaaaacc gggaatgcgg cgacgaagca caccagcgca aggtacccgc ggcaaatgct
     2281 gatcgtgccc gtcagctttt tgaaaaaaga aagcgggatg aaaatgaaaa taagattgat
     2341 gaacagggga gtggtgatcc agatccattc ggggatgtaa ttccacggga tctcccagcg
     2401 gtggatccat cgtccttcga aaagattgta cgaatcgaac ttatcgtagt tgctgaattc
     2461 gctcagcgcg atgaacgggt gcgtgaacgg gttggtgagc ccgtacggcc agaagatcat
     2521 gccgagaaaa taaccggcca cagcggcgat aagcgggtaa cgcaggtgtt tcagcaggaa
     2581 ggaaatcgtt gcggaagtgt ttttcttttc ttttcgcgca atgaagacca tccggaagaa
     2641 ccagcccatg gcgatatacg caatgagcaa aagtc
//
LOCUS       DASTSF010000556         2204 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_107079,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000556 DASTSF010000000
VERSION     DASTSF010000556.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2204
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107079"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="VFU15_15810"
     CDS             <1..194
                     /locus_tag="VFU15_15810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719310.1"
                     /translation="SELKKFCSSVHFFPLGRVTIFFNLLSGLFSGIPLQVAYFTNGKT
                     KKRSPRSSMKKNRTASFAS"
     gene            251..1027
                     /locus_tag="VFU15_15815"
     CDS             251..1027
                     /locus_tag="VFU15_15815"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU4719311.1"
                     /translation="MDVFSKGVERRISKVAAWKRGAFRLEWKRLMRYEKNIFPFFQGH
                     TIISGQDLELLPVENKSGIAVVPNGVDMDYFSPEQKEKDFDLLFNGNMNYPPNIESVV
                     YLAEKILPLVWKKKPGVKLLISGASPSPEVLALRSERISVTGWVKDVRDNFRRSKILV
                     APMQSSIGLQNKLLEAMAMRLPCITTSLSNNALKAIPDSQILVADTPEQFSNHILFLL
                     GHPEKAAQLAENGYQMVKRRFNWEQTCALLENVISGSSLS"
     gene            1148..1501
                     /locus_tag="VFU15_15820"
     CDS             1148..1501
                     /locus_tag="VFU15_15820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719312.1"
                     /translation="MKRLFIILLIAAPLLSMTAVKSWNYLNPEIVPDLKPYDVALSNS
                     DLDKYRYFDKRNTLHFDTGLDVELLSANEMIAAGMPVNKDHVRTQEPSFDTKPVFKLT
                     TDGKLLEVQTRIKIK"
     gene            1509..>2204
                     /locus_tag="VFU15_15825"
     CDS             1509..>2204
                     /locus_tag="VFU15_15825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719313.1"
                     /translation="MKKCYASFLCFFIFCSAASFAQVANDDCFGATPLGTLGAPAACP
                     SGLGAVSTFNNLSNVNSVTETPYTTLINCQPTNNTPMASPATDVWYSFVNNGNQVNIT
                     INGNIQNPNIAIYQGTCNGLIGRGCAIGSGGSLTASFDQMVIGQTYYIQVSGGTPADQ
                     GTFTMTLQNQNSCADCLLASNLTVNPPPTNGTYQGGTTVTFCYTITQWDQQNTNWLHG
                     VVPNFGSGWNMATL"
BASE COUNT          567 a          596 c          542 g          499 t
ORIGIN      
        1 aaagcgagct gaaaaaattc tgcagctccg tgcacttttt tccgcttggc cgcgtcacca
       61 tcttcttcaa tcttctcagc ggactcttca gcggaattcc tttgcaggtg gcctatttta
      121 ccaatggcaa aacgaaaaaa agatcgccgc gatcatcaat gaagaaaaac cggaccgcat
      181 cttttgccag ctgatacgta cggcggaata tgcgaaaaac atccggggca tcccgaaagt
      241 catcgactac atggatgttt tttcgaaagg cgtcgaacgg aggatttcca aagtcgcggc
      301 atggaaacgc ggcgcgttcc gcctggaatg gaaacggctg atgcggtatg aaaaaaacat
      361 cttccctttt ttccagggac atacgatcat ctccggccag gatctcgagc tgctcccggt
      421 agagaacaag tcgggcattg ccgttgtgcc gaatggcgtc gacatggatt atttttcgcc
      481 ggaacaaaaa gaaaaagatt tcgacctgct cttcaacggc aacatgaatt acccgccgaa
      541 catcgaaagc gtcgtttacc tcgcggaaaa aatccttcca ctggtatgga aaaagaaacc
      601 gggcgtaaag ctgctgatct cgggtgcatc accgtcaccg gaagtgcttg cgctgagatc
      661 ggaaagaatt tccgttaccg ggtgggtgaa agatgtgcgc gataatttcc gccgctcgaa
      721 aattctcgtc gcacctatgc agtcgagcat cgggctgcag aacaagctgc tcgaagccat
      781 ggcgatgcgg cttccgtgca tcaccacttc gctttccaac aacgcgctga aagcgatacc
      841 ggacagccag atcctcgtgg ccgatacgcc ggaacaattt tccaaccaca ttctttttct
      901 tctcggtcat cctgaaaaag ccgcgcagct cgcggagaac gggtaccaga tggtgaagcg
      961 acgctttaac tgggaacaaa cctgcgcact gctggaaaac gtgatttccg gctcctccct
     1021 cagctgatct gcggagaaaa tccgctgata ttcaagcacc cccgttgaaa aaactgatat
     1081 ttcccttttg tcgggccgta aaataggcgt atttttattt gtactttcat cccttaaacg
     1141 cctgaacatg aaacggttat ttataattct gttgattgct gcgccattgc tgtcgatgac
     1201 agcggtgaaa tcgtggaatt atctcaaccc ggaaatcgtt ccggacctga aaccttacga
     1261 tgtcgcgctt tccaattccg atcttgacaa gtacaggtat tttgacaagc gcaacacgct
     1321 tcatttcgat acaggtctcg acgtggagct tctttccgcc aatgaaatga ttgcggccgg
     1381 aatgccggtg aacaaagatc acgtacggac gcaggaaccg tcctttgaca ccaaacctgt
     1441 attcaagctc acgacggatg gcaagctgct ggaagttcag acacgcatca agataaaata
     1501 aacaggccat gaaaaaatgc tacgcttctt tcctgtgctt ttttattttc tgcagcgccg
     1561 cttcgtttgc acaggtggcg aatgacgact gtttcggcgc cacgccgctc ggtacgctcg
     1621 gagcgccggc cgcatgtccc agcggactgg gcgcagtttc cacgttcaac aacctttcga
     1681 acgtgaattc cgtaacggaa acgccttaca cgacgctcat caattgccag ccgacgaaca
     1741 atacaccgat ggcatcaccg gccacggatg tatggtattc gttcgtgaac aacgggaacc
     1801 aggtcaacat cacgatcaac ggaaacatac agaacccgaa catcgccatt taccagggca
     1861 cgtgcaacgg ccttatcggt cgcggatgcg cgatcggatc aggcggttct cttaccgcga
     1921 gcttcgacca gatggtgatc ggtcagacgt actacatcca ggtgagcggc ggaactccgg
     1981 ccgaccaggg cacattcacc atgacattgc agaaccagaa cagctgtgcg gactgtcttc
     2041 ttgcttcgaa tcttacggta aatcctccgc ctacgaacgg aacgtaccag ggcggaacta
     2101 cggtcacgtt ctgttataca atcacgcagt gggaccagca gaacacgaac tggctgcacg
     2161 gcgttgttcc caatttcggc agcggatgga acatggcaac gctt
//
LOCUS       DASTSF010000557         2447 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1584008,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000557 DASTSF010000000
VERSION     DASTSF010000557.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2447
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1584008"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..293
                     /locus_tag="VFU15_15830"
     CDS             <1..293
                     /locus_tag="VFU15_15830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HEU4719314.1"
                     /translation="ARPFDPYPFILLNLILSCLAAIQAPIIMMSQNRQEQKDRIRSEH
                     DYKVNLKAELEIKLLHDKMDHLMIHQSRRLLEIQQIQADFLEDIMDEIKKEK"
     gene            453..1394
                     /locus_tag="VFU15_15835"
     CDS             453..1394
                     /locus_tag="VFU15_15835"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEU4719315.1"
                     /translation="MPPRIEGTIVHTTHLSEQVTVPGNVLPWDEAVLMPEVAGRITYL
                     RLPEGQHVTKGTLLVKLYDGDLQAQLKKLQGQLLNAESVVKRQGELVKMNGVSQADYD
                     ASVMQLTNVQSDIAAVNAQISKTEILAPFDGVIGLRKVSEGAYVSPGTALATIRSDNE
                     FKLDFSVPEIYASLVNEGMKVSFTSGNDTVQYAAEIIATEQSVDESSLGLNVRAKISG
                     KAKKIVPGSSVTVTLGVGETDHAVVIPSQSIIPQARFKSVIVSRNGKAQYVNVRTGIR
                     TANGVQIVSGLNEGDTIATTGIQFIRPGMPLRFSSIK"
     gene            1401..>2447
                     /locus_tag="VFU15_15840"
     CDS             1401..>2447
                     /locus_tag="VFU15_15840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEU4719316.1"
                     /translation="MSIGGNSLKRPVGSIVLSLLIILFGVVGYNFLGVREYPAIDPPT
                     ITVRTPYTGANAEIIESQITEPLEKAINGIEGIKSVSSSSAVGVSNITVEFELGKDLE
                     KAANDVRDKVSQAVKNLPQDIDAPPTVTKSDANSDPIIFMPIQSTKMTDLELSDYVEN
                     VMQEKLQTIPGVSEVRIYGQRRPSIHLWIDPQKLAARKLSVQDVQDALTRENIELPGG
                     KIEGNKTELTIKTFGRLTTEDDFNNMIISDKDNVVTRFSDVGEAVLGPENEETISRLS
                     NSTAVSVAIIPQPGANYIEISNEFYKRFDEIKKNLPDGMTIDVGIDKSNFVRQSISEV
                     KETLLIAISLVILII"
BASE COUNT          638 a          656 c          643 g          510 t
ORIGIN      
        1 ccgcgcgtcc tttcgatccc tacccgttca tcctgctcaa ccttattctt tcctgccttg
       61 ccgccataca ggctccgatc atcatgatga gccagaatcg ccaggaacag aaagaccgta
      121 tccgcagcga gcacgattac aaggtgaacc tgaaagctga gctggaaata aaactgcttc
      181 atgataaaat ggatcacctc atgatccacc agagccggcg acttctcgag atccagcaga
      241 tacaggctga tttcctcgag gatattatgg acgagataaa aaaagagaag taatgttctt
      301 tttagtgatg aacggtaatt ccatgttttg acattcaagt atctttgcgc atgttttttc
      361 cgaatcccgc caggagtatt tttcttttca gcccccttgt tattcttgct tcttgttccg
      421 gcaacggtgg taaaacaccg gagaagaaga acgtgcctcc caggatcgag ggcacgatcg
      481 tacatacaac gcatctcagc gagcaggtga ccgtcccggg aaatgttctt ccctgggatg
      541 aagccgtgct catgccggaa gtcgcgggaa ggatcaccta cctgcgcctg cccgaaggac
      601 agcatgtcac gaaaggcacg ctgctcgtca agctgtacga cggagatctc caggcgcagc
      661 tgaaaaaatt acagggccag ctcctgaatg cggagagcgt ggtgaaacgc cagggagagc
      721 tcgtgaagat gaacggtgtc agccaggcgg actacgacgc gtcggtcatg cagctgacca
      781 atgtgcagtc ggatatcgct gcggtgaatg cacagatcag caaaacggaa attctcgctc
      841 ccttcgacgg tgttatcggt ctccgcaagg tgagcgaagg cgcatacgtt tcacccggaa
      901 cggcgctcgc cacgatacgc agcgataacg agttcaaact cgatttttca gtcccggaaa
      961 tatatgcatc gctcgtaaac gagggcatga aagtcagctt cacttcgggg aacgacacgg
     1021 tgcagtatgc cgcagagatc atcgccaccg agcagagcgt ggatgaaagt tcgctcggtc
     1081 tcaacgtacg tgcgaagatc agcggcaaag cgaaaaagat cgtgcccgga tcttctgtca
     1141 cggttacgct cggtgtcggc gagacagatc atgcggtagt aattccttca caaagcatca
     1201 tcccgcaggc gcgtttcaaa agcgttattg tttcgcgtaa tggcaaggcg cagtatgtga
     1261 acgtgaggac cggcatccgg acggccaacg gcgtgcagat cgtttccggc ctgaacgaag
     1321 gcgacactat cgcgacaaca ggaatacagt tcattcgtcc cggaatgccc ctccggtttt
     1381 ccagtataaa ataaccgggc atgtcgatag gcggaaattc tctcaagcgt cccgtaggtt
     1441 ccatcgtgct gagcctgctg atcattcttt tcggcgtggt cggttacaat tttctcggcg
     1501 tgcgcgaata cccggctatc gatcctccga cgatcaccgt ccgcacaccg tataccggcg
     1561 ccaacgcaga gatcatcgaa tcacagatca ccgaaccgct cgaaaaagcg atcaacggga
     1621 tcgaagggat caaatccgtc agctcctctt ctgccgtcgg cgtgagcaac atcaccgttg
     1681 agttcgagct cggaaaagat ctcgagaaag cggccaacga cgtgcgcgac aaagtttcgc
     1741 aggctgtaaa aaatcttccg caggacatcg acgcgccgcc gaccgtcacc aaatccgatg
     1801 cgaacagcga cccgatcatt ttcatgccga ttcaaagcac gaagatgacc gacctcgaac
     1861 tgagcgatta cgtggaaaac gtgatgcagg aaaagctgca gacgatcccc ggggtgagtg
     1921 aagtccgcat ctacgggcag cgacgcccct ctattcacct ctggatcgat ccgcagaaac
     1981 tggcggcgag gaaactttcc gtgcaggacg tgcaggatgc gctcacgcgc gagaacatcg
     2041 agcttcccgg cggaaagatc gaaggaaata aaacggagct gacgataaag actttcgggc
     2101 gtcttacgac ggaagatgat ttcaacaaca tgatcatctc ggacaaggac aacgtagtga
     2161 cgcggttcag cgacgtggga gaagcggttc tcggtccgga aaacgaagaa accatttcac
     2221 gtctcagcaa ttcaacggcg gtttccgttg cgatcattcc gcagccggga gcgaattaca
     2281 tcgagatatc caatgaattt tacaagcggt ttgacgagat caaaaaaaat cttcccgacg
     2341 ggatgacgat cgatgtgggc atcgacaaat cgaatttcgt ccgccagtcc atttcggaag
     2401 taaaggaaac cctgctgatc gccatctcgc tcgtcatcct tatcatt
//
LOCUS       DASTSF010000558         4116 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_121157,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000558 DASTSF010000000
VERSION     DASTSF010000558.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4116
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121157"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3256)
                     /locus_tag="VFU15_15845"
     CDS             complement(<1..3256)
                     /locus_tag="VFU15_15845"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type B sorting domain-containing protein"
                     /protein_id="HEU4719317.1"
                     /translation="MRFPLRALLLLFFLAVFSPRLSASHLMGGEITWACQGNGQFVFN
                     MKLYRDCNGIPGPLSAALDVYNNPNVSSITMNLVSQTDISPQCNSIGPSITCANASST
                     NPLPGAVEEYIYASNPITLSGVPPAAGWVFVFSSCCRNTAITNLAQSAGGSGFTLRAI
                     MHAYNNQNENPCFDNSPEFLESPRTIICTGYPFTYNHNAVDPELDSLAYSWAQPLDQI
                     TPPATWNPPVSPAAIPFVAGFSYNSPMPGTNLDPNNVPATMNPYTGEISYTSFTSGNF
                     VTCVKVEAWKCGQLVAEIYREIQVVLLPCGNNTPPSVIPPFAANTVFQDTVYAGQLVN
                     FTLTATDFDFLPNGQPQTLTINASGQEFGANFTNPASGCLNPPCATLNPPPPVSGPFG
                     VSTDFSWQTACNHVNYTNQCFSLSNTYNFVLRTSDDFCPAPAQTIATISITVLNLPIV
                     LSPHVHCVAVAPNGDVTLTWDQPVDTAGTFDSYHIFSSPNPNGPFTEIDSIFNYNQLS
                     YTHTGANANNGSVYYYVETRSGCGGQVIMPPQDTVRSIFLNVTNSNNVAALLTWNNIS
                     NPPPSTQYPWFRIYREYPTGNWVLIDSTQSLNYVDSIQVCNNQINYRIEIGDSLGCVS
                     VSNVDGDHFTDNVIPQIPQLDSVSLDSNDLAVIGWNQSASPDVIGYVVYQYLNGIWTA
                     LDTIWGRGNTSYTNLISNAGQQSEQYEVAALDSCGNISSGGGAHNTLFLNSALDICNS
                     TANLWWNSYVNFPGGLGGYEIYACENGGPYSLIGTTVAGTTTYADSMLNSFSTYCYYV
                     VAFNSAHTIGSRSQKVCVYASVPQQPVFNYFRVATVTGQNSVLLKGYVDVNADVQRYD
                     FYRTDTLTNAWTLIGSVPASPLVSTISFTDNDVETDVRSYYYRMMAIDSCGNPSLISN
                     VAHTVFLQAFANDEITNTLMWNDYSTWDGSVMSYNVYRSIDGVLDPVPVATVPFANAG
                     TNVYTDDVSAYINYIGKFSYLVEAVENPGNQYGLTDTAYSNYADVVQKPLVYVPNAFS
                     PNGDGLNDIFIPSNGFIDVQDYDFAVFNRWGEKLFQTNDQSQGWDGHFGGKRCDP"
     gene            3399..>4116
                     /locus_tag="VFU15_15850"
     CDS             3399..>4116
                     /locus_tag="VFU15_15850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010600693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2071 domain-containing protein"
                     /protein_id="HEU4719318.1"
                     /translation="MEASVNSAGNAPVFLRAEWRKLMIVNYAVLGDLLGEYIPHHTEP
                     DLFNGNCLVSLVGFRFINTKLKGIPVPFHRNFTEVNLRFYVRRNLPGGICRRGVVFIR
                     EIVPLPALSLVANLVYGEHYETRPVRHELASEAGAISVAYEWKKKAKHRISVVAENNA
                     EEIKPFSAEEFITEHYWGYTKLRGNKTAEYAVEHERWKSYPVKEYEVKVDFGLSYGKK
                     FAFLSGEKPVSVMLAEGSEII"
BASE COUNT         1024 a         1063 c         1128 g          901 t
ORIGIN      
        1 ccgggtcgca gcgcttcccg ccgaaatgcc cgtcccatcc ctgcgactgg tcattggtct
       61 ggaacagctt ttctccccag cggttgaata ccgcgaaatc ataatcctgc acatctataa
      121 aaccgttcga tggaatgaaa atatcgttca ggccgtcccc gttcggcgaa aaagcattcg
      181 gaacatacac gagcggcttc tgcaccacat ccgcatagtt ggaataagcg gtatccgtaa
      241 gcccgtactg gttgcccgga ttttccaccg cctcgaccag gtagctgaac ttcccgatgt
      301 agttgatata cgcgctgacg tcgtccgtgt aaacattcgt acccgcattg gcgaacggaa
      361 ccgtcgcaac cgggacagga tcaagcacac cgtcaatgct gcggtacaca ttgtagctca
      421 ttacgctacc gtcccaggtg ctgtaatcat tccacatcag cgtgttcgtg atctcgtcat
      481 tggcaaaagc ctgcaggaaa accgtatgcg ccacgttcga gatcagcgac gggtttccgc
      541 agctgtcgat cgccatcatc cggtagtaat agctgcgcac atccgtttca acgtcattat
      601 ccgtaaacga gatcgtgctc accagcggcg acgcaggaac acttccgatc agcgtccatg
      661 cattcgtcag cgtatccgtc cggtaaaaat catagcgctg cacatccgca ttcacgtcca
      721 cgtagccttt cagcaaaact gaattctgtc ccgttaccgt cgcaacgcgg aagtaattga
      781 acaccggttg ctggggaaca gaagcgtaca cgcacacttt ctgcgagcgc gatccaattg
      841 tatgcgccga attgaacgca actacataat agcaatacgt ggaaaatgaa ttcagcatgg
      901 aatcggcgta agtcgtggtt cccgctaccg tcgttccgat cagcgaataa ggcccgccat
      961 tctcgcaggc gtaaatttca tacccgccaa gaccgcccgg aaaattcaca taactgttcc
     1021 accacaggtt cgcggtggaa ttgcagatgt ccagcgcaga attcaggaaa agcgtattgt
     1081 gcgcacctcc gccggagctg atgttgccgc agctgtcgag cgccgccact tcatattgtt
     1141 ccgactgttg tcccgcattt gaaataaggt tggtgtacga ggtgttcccg cgtccccaga
     1201 tcgtgtccag cgccgtccat attccgttca ggtactggta aacgacgtaa ccgatcacat
     1261 ccggcgaggc gctttggttc catcctatca ccgcaagatc attcgaatcc agcgaaaccg
     1321 aatccagctg cgggatctgc gggatcacgt tgtcggtaaa atggtcgcca tccacattgg
     1381 aaacagaaac acaccccagc gaatcgccga tctcgatgcg gtagttgatc tggttgttgc
     1441 acacctggat cgaatcgaca taattcagcg attgcgtgga gtcgatgagc acccagttgc
     1501 cggtgggata ttcccggtaa attctgaacc agggatattg cgtggacgga ggcggattgc
     1561 tgatgttgtt ccacgtaagc aaagcggcta cattgttgct gttcgttacg ttaaggaaga
     1621 tcgatcgcac cgtatcctgc ggcggcatga tcacttgtcc gccgcagccc gaccgcgttt
     1681 ccacataata atacacactc ccgttattgg cattcgcgcc ggtatgcgtg taggaaagct
     1741 ggttgtaatt aaaaatcgaa tcgatctccg tgaaaggccc gttcgggttc ggcgaggaaa
     1801 aaatatgata gctgtcgaat gttccggcgg tgtcaacggg ctgatcccag gtgagcgtga
     1861 catccccgtt cggcgctacg gcaacacaat gcacatgcgg cgaaagaaca atgggaagat
     1921 taagtaccgt aatggagatc gtggcgatgg tttgcgccgg cgccgggcag aaatcgtccg
     1981 acgtgcgcag cacaaaattg taggtgttgg acaggctgaa acactggttg gtgtaattca
     2041 cgtggttgca ggccgtctgc cagctgaaat cggtgcttac accgaagggc ccggacacgg
     2101 gtggcggtgg gttcagcgtt gcgcacggag gattgaggca gccgctcgcg ggattggtga
     2161 aattggcgcc aaactcctgg ccggaagcgt ttatagtcag tgtctgcggc tgcccgttgg
     2221 gcaggaaatc aaaatcagtg gcggtgagcg taaaattcac cagctgcccg gcgtataccg
     2281 tatcctggaa taccgtgttc gcggcaaacg gaggaatcac ggaaggcggc gtattgtttc
     2341 cgcagggcaa aagaaccacc tgtatctccc ggtatatttc cgcaacgagc tgcccgcatt
     2401 tccaggcttc cactttcaca caggtcacaa aatttccgga ggtgaacgaa gtataggaaa
     2461 tctcccccgt gtaagggttc attgtcgcag gaacgttatt cggatcgaga ttggtccccg
     2521 gcatcgggct gttgtaggaa aaaccggcca caaacgggat cgcagcaggc gatacgggcg
     2581 ggttccacgt ggcgggcggt gtgatctggt caagcggctg cgcccacgaa taggccagcg
     2641 aatccagctc gggatccact gcgttgtgat tgtacgtaaa cgggtaacct gtacagatga
     2701 tcgttctcgg gctttcgagg aattccggag aattgtcgaa acacgggttc tcgttctggt
     2761 tattatatgc atgcataata gcacgcagcg tgaatcccga tccgcctgcc gactgcgcca
     2821 gattggtgat cgccgtattg cggcagcagg aagaaaaaac gaaaacccag ccggcagccg
     2881 gcggaacacc ggagagcgta atgggattgg aagcataaat atattcctcg accgcgccgg
     2941 gcaagggatt cgtgctggac gcgttcgcac aggtgatgga aggtccgatc gaattgcatt
     3001 gcggggaaat atccgtctgc gaaacgaggt tcatcgtgat cgatgaaacg ttcgggttat
     3061 tgtacacatc gagcgcggct gaaaggggac ccgggatacc attgcaatcc cggtaaagtt
     3121 tcatattgaa aacaaactgg ccgttgccct ggcaagccca cgtgatctct cctcccatca
     3181 ggtgcgatgc agacagccgc ggagaaaata ccgcaagaaa aaacaacaaa agcaaagcgc
     3241 gcaagggaaa gcgcatgaat tcctgtgatt agcctgaata acaaagatag gattttagct
     3301 ccgggtgaac gtgttgtttt tgactactta ttgcgcagta ttgaccggca atgaacaaat
     3361 gatttcctac tttggacatt atagcaaaca catcgcggat ggaagccagc gttaattcag
     3421 cgggaaatgc tcccgttttt ctcagggcgg aatggagaaa gctgatgatc gtgaattacg
     3481 cggtgctggg agacctgctc ggtgaataca tccctcacca tacggaaccc gatcttttca
     3541 acgggaactg cctggtgagc ctggtcggat tccgattcat caacacgaag ctgaaaggca
     3601 ttcctgttcc gttccaccgg aatttcaccg aggtgaacct gcgtttttat gtacgaagaa
     3661 accttcccgg cggtatatgc agacgcggcg tggttttcat cagggagatc gtgcccctgc
     3721 ccgctttgtc gcttgtcgcc aaccttgttt acggcgaaca ttacgaaaca cgccccgtgc
     3781 ggcatgaact ggcaagcgaa gccggagcaa tctcggttgc gtacgaatgg aaaaagaaag
     3841 cgaaacaccg catttcggtg gtggcggaaa acaacgcgga agaaataaaa ccattctctg
     3901 cggaagagtt catcacggaa cattactggg ggtatacaaa actgcggggg aataagactg
     3961 ccgaatacgc ggtcgagcat gaacggtgga agagctaccc ggtaaaagaa tacgaagtga
     4021 aagtggattt cgggctgtcg tacggaaaaa aattcgcctt cctgtccggt gaaaaacccg
     4081 tttccgtcat gctcgcggaa ggttcggaaa ttatcg
//
LOCUS       DASTSF010000559         7316 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_330036,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000559 DASTSF010000000
VERSION     DASTSF010000559.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 7316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7316
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_330036"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1139)
                     /locus_tag="VFU15_15855"
     CDS             complement(<1..1139)
                     /locus_tag="VFU15_15855"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEU4719319.1"
                     /translation="MKKLFSLFLLTLPLLSLAQNTGKTVDRIVGVVGGQIVKESDVEN
                     AYQQFKGAGVPMSDSLRGVIMDQLLYKKLLINQAKHDSIDVSEDEINAETDRRMRYFL
                     SQFKSEKEFTDFYGKSVDAFKFELHDQVRDLLLAQKMEGKITGDITVSPADVAAYYNS
                     QPPDSLPFISSEVEVGQIVVVPPVSQELKDYARQTLEDVRTRVVSGKLDFCSAAAIYS
                     DDPGSKFNCGKYENVRRGTFVPEFDAVCFSLKEGEISDVFETDYGFHFVKLISRRGEE
                     VTIQHILKAIPVDPDELKKCKLKLDTITSLIRKDSLTFCEAAAKYSADADSKYSCGMI
                     VNPETGTTKIDVEMLGQLDPDPQFPLVVNQMKVGELSAPQPCVTR"
     gene            complement(1142..2056)
                     /locus_tag="VFU15_15860"
     CDS             complement(1142..2056)
                     /locus_tag="VFU15_15860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719320.1"
                     /translation="MWELPAIRHCRQLINRSSVFLWGTLAFCFALSCRPPQDDKQETE
                     NRGKVIARVFEYKLYEADLREVIPAGLSAEDSTRRAENFINAWVKEKILVHKAELNLG
                     PDQKNIDKQLQDYKNSLIIYAYEKELVRQQLDTVVGDEEIQAYYDNHPNDFELKDNII
                     KVIYVKVDRKAPNIPKLKNLILSDKPQDRKDLDNYCRQFASNFYLDDNSWLLFDDLLK
                     EVPIETYNKELFLKNNRFVEVEDSSSLYFVNIKGFMTRNSHSPLAFEKDNIRNIIINK
                     RKRELIDKMQGDLYKQAMENKEVEIYHR"
     gene            complement(2063..4222)
                     /locus_tag="VFU15_15865"
     CDS             complement(2063..4222)
                     /locus_tag="VFU15_15865"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEU4719321.1"
                     /translation="MKKHNLLIAFALFLFSAAFAQSPKSAGSAPGKLVPSPALVVAAD
                     TDTSDPVLMTIGNSQVHLGEFMYVYKKNNKDQSNDPQALNNYIDMFTVFKMKVKEAED
                     MKLDTSAAFRNELLGYRRQVAQQYLTDNKVNDSLLQEAYARMQEDVRASHILVRCDEN
                     ALPKDTDLAWTHISLIDGLLTGKTTAKQISDYEMHLKASMNISKTSKPADTLKVYNII
                     NPLRQLQRKYNGKAAPFDEVATMTSEDESARMNHGDLGYFTAFSMVYPFETAAYNTPV
                     GKVSNPVRTRFGYHLVKVTDRRPAQGEILVAHIMIKAPDGMPAADSIAAKKKADEIYA
                     KAIAGANFDTLARQFSDDKASAVNGGQLPWFGLYKMPQPFERAAFSLQNNGDISAPVK
                     TAWGWHIIKRIDKRGIPPFDQVKADIKNKVSRDQRAMQGRAALIARVKAENNFTETPK
                     AKDDFYKVVDSTYFNGKWTADRAKKLTKTMFTLAGVAYTQQEFAIWLENHEVRRPKTD
                     VKKVVDDAYKQFVDEACVNYEDSQLEKKYPAFRYLMQEYRDGILLFDLMDKNVWSKAV
                     KDTAGLRAFYDANKTNYMWPERSDATIYSCKDAKTAKKVRKMIKDGKDQKAILDAMNK
                     DSQLNVTADHKVWNKNENAEVDANWKKGVSLDIQKDGRVLIIVTNEIIPPTPKTLQEA
                     RGMVTSDYQNQLEKDWVAKLKAKYPVTVDQQVLKQVK"
     gene            4680..5540
                     /locus_tag="VFU15_15870"
     CDS             4680..5540
                     /locus_tag="VFU15_15870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719322.1"
                     /translation="MKMRYAAILPLFFLLACSNDQPATNNVDTTPKKDTTQVADDEDM
                     PESTLPSPLAVASMFKRSGLKYLPGLINTTDKAGTYSTMFSRAENMGVYSSDMAYCVM
                     NKQSNEGGQLLKVIRDLGKQINLGNVFDQGNLYDRFNKNLDNEDSLGKIIADIQFETD
                     KELAKNDQNDLYGVIFAGAWIEAMYVGCEVYKKDGNDDVVAALVEQMVVSRNIVTELK
                     NYETKDPSMPGLIKDMTDIRDAINAMPSMKKLDDDQDIDFKDVHPTKEELAPVIKKIE
                     DLRNKMISNG"
     gene            5561..6028
                     /locus_tag="VFU15_15875"
     CDS             5561..6028
                     /locus_tag="VFU15_15875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719323.1"
                     /translation="MKRSFLFILPAIVAFVALTAAAIQTAETCDQNALKAKAKAALDP
                     FKYDSGKVTRIYYRKKAQVKEIEVPVFVGEKYRFVFNSEAVTRDVTITVYNKDKDSKN
                     RKALFTMKALDGNRVQTFDPDGARMHFFVDYDIPATNDSLPPADCLVMMLGYK"
     gene            6374..6691
                     /locus_tag="VFU15_15880"
     CDS             6374..6691
                     /locus_tag="VFU15_15880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719324.1"
                     /translation="MAKQMLEMNTGIRIIPVNAERMLNGSHGFANAEFDKWVQFKFTD
                     ISEPLAQVWTGIPFRFIARLEEMNEGRPVRNYALRQLAEALIEHEPSFDPVNDYVYIC
                     PGE"
     gene            complement(6729..>7316)
                     /locus_tag="VFU15_15885"
     CDS             complement(6729..>7316)
                     /locus_tag="VFU15_15885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014796291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HEU4719325.1"
                     /translation="GGDWYANLETSLPNLIKFCNDNIKTNINPEQAIVEVGSPDIFNY
                     PFVHMTGHGNVIFSQQEADNLRNYLTGGGFLHISDNYGLDQFIRPQMKKVFPELDFVE
                     LPFSHPIYHQVYDFPNGLPKIHEHDGKPAQGFGLIWEGRLVCFYDYQCDLGDGWESPE
                     VHHDSQEIRLKALKMGANLVNYALMGGESKHDEAQ"
BASE COUNT         1586 a         1964 c         1864 g         1902 t
ORIGIN      
        1 tccctcgtca cgcaaggctg cggcgcagaa agttcgccca ctttcatctg gttcacaacg
       61 agcgggaatt gcggatcagg atcgagctgg cccagcattt ccacgtcgat cttcgtcgtg
      121 ccggtttccg ggttgacgat cattccgcag ctgtatttcg aatcggcatc tgcagaatat
      181 ttcgcggctg cttcacagaa cgtcaacgaa tctttccgga tcagcgaagt gatcgtgtcc
      241 agcttcagct tgcatttctt caattcatcc gggtcgacgg gaatggcttt caggatgtgc
      301 tggatcgtca cctcttcacc gcggcgcgag atgagcttaa cgaaatggaa accgtaatcg
      361 gtttcgaaga catcggaaat ttcaccttct ttcaggctga aacagacggc gtcgaattcg
      421 ggaacgaatg tcccgcggcg gacgttttcg tacttgccgc agttgaattt cgaacccgga
      481 tcatcggaat aaatcgcggc ggccgaacag aaatcgagct tgcccgacac cacgcgcgta
      541 cgcacatcct cgagcgtctg gcgcgcgtaa tccttgagct cctggctcac cggcggaacg
      601 acgacgatct gtcccacttc cacttccgag ctgatgaacg gcaagctgtc cggcggctgg
      661 gaattgtaat acgccgcgac atcggcaggt gaaacggtaa tgtcgcccgt gatctttcct
      721 tccattttct gcgcaagaag aagatcgcgc acctggtcgt gcagctcgaa cttgaacgcg
      781 tcgaccgact tgccgtaaaa atccgtgaac tctttttccg atttgaactg gctgagaaaa
      841 tagcgcatgc ggcggtccgt ttccgcgttg atctcatctt ccgaaacgtc gatcgaatcg
      901 tgcttcgcct ggttgatcag cagtttcttg taaagcagct ggtccatgat cacgccgcgc
      961 aggctgtccg acatcggcac gccggcgcct ttgaactgct ggtacgcatt ttccacgtca
     1021 gattccttga cgatctgccc gccgaccacc ccgacgatcc tgtcaacggt cttgccggta
     1081 ttctgcgcca ggctgagaag ggggagcgtc aataaaaaaa ggctgaagag ttttttcatt
     1141 tttaccggtg atagatttct acttccttgt tttccatcgc ctgcttgtac agatcgccct
     1201 gcattttgtc gatcagctca cgcttgcgtt tgttgatgat gatgttccgg atgttgtctt
     1261 tttcaaaggc caggggcgaa tggctgttcc gtgtcatgaa tcctttgatg ttgacgaaat
     1321 acaggctgga tgaatcctcc acctccacga agcggttgtt cttgaggaac aattccttgt
     1381 tgtaggtttc aatgggcact tccttgagca ggtcgtcaaa aagcagccag gaattatcat
     1441 cgaggtaaaa gttcgacgca aactgccggc agtaattatc gaggtctttc cggtcctgcg
     1501 gcttgtccga aaggatcagg ttcttcagct tgggaatgtt cggcgccttg cggtcgacct
     1561 tcacgtagat cactttgatg atgttgtcct tcagctcgaa atcattcgga tgattatcgt
     1621 aatacgcctg gatctcttcg tcgccgacaa ccgtatcgag ctgctggcgc accagctcct
     1681 tctcgtaagc gtaaatgatc agcgaattct tataatcctg caattgcttg tcgatgtttt
     1741 tctggtcggg accgaggttg agctcggctt tgtgcaccag tattttttcc tttacccacg
     1801 cgttgatgaa attttccgcc cttctcgtgc tgtcttctgc agacaggccg gcggggatca
     1861 cttcccggag gtccgcctcg tacagcttgt attcgaaaac ccgcgcaatc acttttccac
     1921 ggttttccgt ttcctgcttg tcatcctgcg ggggacggca cgacagggca aagcaaaaag
     1981 ccagcgtccc ccagaggaag acgctgcttc gattgatcag ttgcctgcaa tgccggatcg
     2041 ccggtagttc ccacatgaag ggctatttca cttgtttaag tacctgctgg tcgacagtga
     2101 cgggatactt tgctttcagc ttcgccaccc agtctttctc gagctggttc tggtaatcag
     2161 aggtgaccat ccctctcgct tcctgcaatg ttttcggggt aggcggaatg atctcgttcg
     2221 taacgatgat cagcactctg ccgtccttct ggatatcgag ggaaacgccc ttcttccagt
     2281 tggcgtccac ttccgcattt tcattcttgt tccacacttt gtggtcggcc gtgacattga
     2341 gctgtgaatc tttgttcatc gcatcgagga tcgccttctg gtcttttccg tccttgatca
     2401 tcttgcgcac tttcttcgcg gttttcgcat ccttgcagga atagatcgtc gcatcggaac
     2461 gctccggcca catgtagttg gttttgttcg cgtcatagaa tgcgcggagg ccggccgtat
     2521 ctttcaccgc tttcgaccag acgttcttgt ccataagatc gaacagcagt attccgtcgc
     2581 ggtattcctg catgaggtag cggaacgccg ggtatttttt ctcgagctgg ctgtcttcgt
     2641 aattgacgca ggcctcatcc acgaactgct tgtaggcatc gtccaccact tttttgacat
     2701 cggttttggg ccggcgcact tcgtgattct cgagccagat ggcgaattcc tgctgcgtgt
     2761 aagcgacgcc cgcaagcgtg aacatcgttt tggtcagctt cttcgcacgg tcggcggtcc
     2821 atttcccgtt gaagtacgtg ctgtcaacca ccttgtaaaa atcgtctttg gctttcggag
     2881 tttcggtaaa attattttcc gctttcacac gggcaataag cgccgctctt ccctgcatgg
     2941 cgcgctgatc gcggctcacc ttgttcttga tgtccgcctt tacctggtcg aacggaggaa
     3001 tgccgcgctt gtcgatgcgc ttgatgatgt gccatcccca ggctgttttg accggtgcgg
     3061 agatgtcgcc gttgttctgc agtgaaaatg cggcgcgctc aaagggctgc ggcattttgt
     3121 agagaccgaa ccagggaagc tgtccgccgt ttaccgcgga tgctttgtca tcggagaact
     3181 ggcgggcaag cgtatcgaag ttcgcacctg cgatcgcttt cgcatagatc tcgtcggctt
     3241 tctttttcgc tgcgatggaa tcagcggccg gcattccgtc gggcgccttg atcatgatgt
     3301 gtgcgacgag gatctcgccc tgcgcgggac ggcggtccgt cactttcacc aggtgatacc
     3361 cgaaacgcgt gcgtaccggg ttgctgactt tgccgaccgg cgtattgtac gcggccgttt
     3421 caaacgggta caccatgctg aatgcggtga aatacccgag gtcgccgtga ttcattctcg
     3481 ccgattcgtc ttccgaggtc atcgtcgcca cttcgtcgaa cggggccgcc ttgccgttgt
     3541 atttgcgctg gagctggcgg agcgggttga tgatgttgta cactttcagc gtgtcggcgg
     3601 gtttggacgt ttttgaaatg ttcatcgaag ctttcaggtg catttcataa tcactgatct
     3661 gctttgccgt tgttttcccg gtaagcagcc cgtcgatgag ggaaatgtgc gtccatgcga
     3721 gatccgtgtc tttcgggagt gcattttcgt cgcagcgcac aaggatgtgg ctggcgcgga
     3781 cgtcctcctg catgcgcgca tacgcttcct ggagaaggct gtcgttgact ttgttatcgg
     3841 tgaggtactg ctgcgccacc tggcggcggt agccgagcag ttcgttcctg aatgcggccg
     3901 aggtatccag cttcatgtct tccgcttcct tgactttcat cttgaagacg gtgaacatgt
     3961 cgatataatt gttcagcgcc tgcggatcat tgctctggtc cttgttgttc tttttataaa
     4021 cgtacatgaa ctcaccgagg tgaacctgtg aatttccgat cgtcatcagc accgggtcgg
     4081 aagtgtcggt gtcggcggcg acgacgagtg cgggtgaagg cacaagcttc cctggagcgc
     4141 ttccggcgct tttggggctt tgtgcgaatg ctgctgaaaa aaggaaaagg gcgaaggcaa
     4201 taagcaggtt gtgctttttc atacttgtaa ttgtaagttg cttgaatttt agcggaagca
     4261 aaagtagcga ttttgggtga aaatccccca ccgaaaaaat gtcaaaatca agggattggc
     4321 tctgcggcgt cttctttgtc tttgtcatta acacctaaat aaattccgaa aaaacactgt
     4381 tgtttcgcat aagatcattc agaatacagc acctgatcac ctaatggatt aactatttta
     4441 agtcccggat tcatggttct gaaagttccg taaaaagtaa atccggaaat ccgctgattc
     4501 agcccggtcc ccgacccggt catccggtct ttgatttttt cgatcctcca gcctttgatt
     4561 tttccatccc ttcgctcctt ccggttttta aatcattcaa tccccaattc cctgatttcc
     4621 cgtttcatcc atttttctac ctttgcccgt attcaattaa ctgaaaaaca attcttccca
     4681 tgaaaatgcg ctacgctgcc attctccccc tgttcttcct gcttgcgtgc agcaacgatc
     4741 agcccgccac caacaatgtg gacaccacac cgaagaaaga caccacgcag gttgcggacg
     4801 atgaggacat gcctgaatca acgctgcctt ctccccttgc cgttgcttcc atgttcaaac
     4861 gttcgggcct caaatacctt cccggtctca tcaacacgac cgacaaagcc ggcacctaca
     4921 gcacgatgtt ctcgcgtgcg gaaaatatgg gcgtttacag ctccgacatg gcgtactgtg
     4981 taatgaacaa gcagtcgaac gagggcggac aattgctcaa agtgatccgc gacctcggca
     5041 agcagattaa cctcggcaac gttttcgacc agggcaatct ttacgatcgc ttcaataaga
     5101 atctcgataa tgaagattcg ctcggcaaga tcatcgccga catccagttc gaaaccgaca
     5161 aggaactggc gaagaatgac cagaacgacc tctacggtgt gatctttgcg ggtgcatgga
     5221 tcgaagccat gtatgtaggc tgcgaagtgt acaagaaaga cggcaatgat gatgtggtgg
     5281 ctgcgctcgt cgagcagatg gtcgtatcga gaaacattgt gacggaactg aagaattacg
     5341 aaacaaaaga tccttccatg ccgggactta tcaaagacat gaccgacatc cgcgatgcga
     5401 tcaacgcgat gccctcgatg aaaaagcttg acgacgacca ggatatcgat ttcaaggacg
     5461 tccacccgac gaaagaggag ctcgctcccg tcatcaagaa gatcgaagac ctccggaata
     5521 aaatgatctc aaacggctaa ttccaaaacc cgcatacctc atgaaacgtt cattcctgtt
     5581 catccttccc gccatcgtgg cttttgtcgc gctcaccgcc gctgccatac agacggctga
     5641 aacgtgcgac cagaacgcat tgaaagcaaa agcgaaagct gcgctcgatc cgttcaagta
     5701 cgattcggga aaagtcaccc gcatctatta ccgcaaaaaa gcgcaggtaa aagagatcga
     5761 agtgcctgtt ttcgtcggtg agaaataccg ctttgtgttc aacagcgaag ctgtaacacg
     5821 cgatgtgacg atcaccgttt acaacaagga taaagattcg aagaaccgca aagcgctgtt
     5881 cacgatgaaa gcgctcgacg gcaatcgtgt acaaaccttc gatcccgacg gagcgcgcat
     5941 gcacttcttc gtcgattacg acattccggc cacgaacgat tcccttccgc cggcggattg
     6001 cctggtgatg atgctcggct acaaataaaa aaaccacccc ggccccccgg ggttttttat
     6061 tacatctcct gcggggacat ctccgcaatt tttccgtccc gctgctgtgt tgaaaaaata
     6121 aacggcgtca ttctgcttct tcatcgccga tattaacaaa aactgaagca attgctgacg
     6181 ttttgttttt ccttattcca caagggttcc aggccatttt accgtcgcgc ggcgcctgtt
     6241 ttcaacaacg tttcccccgc ttttccgatt tccgccaaca cgccggtgta gaaaacttcg
     6301 aaacgatata cctgcatgcc atttgcgcgc cttctatttt tgatcgcata caaaatcaga
     6361 aacaggcccg cttatggcga aacagatgct ggaaatgaat acggggatcc gcattattcc
     6421 tgttaacgcg gagcgtatgc tcaacggaag tcatggattc gccaacgccg agttcgacaa
     6481 gtgggtgcag ttcaaattca ccgatatttc ggaacctctc gcgcaggtgt ggacgggaat
     6541 cccgttccgc ttcatcgcac gtctcgagga aatgaatgaa ggccgccccg tgaggaacta
     6601 cgccctgcgc cagctggcgg aagcgctcat cgagcatgag ccttcgttcg atcccgtcaa
     6661 cgattacgtg tacatctgcc cgggcgaata atccttcgca ccgttttcca tttcccggaa
     6721 gaagaaaatt attgcgcctc gtcgtgcttc gattcaccgc ccatgagcgc gtagttgacg
     6781 agattggcac ccatcttcag cgccttcagg cgtatctcct gggaatcgtg gtgaacttcc
     6841 gggctttccc agccgtcacc gagatcgcac tggtaatcgt aaaaacacac cagccggcct
     6901 tcccagatca gcccgaaacc ctgtgcgggt tttccgtcgt gctcgtggat cttcggaaga
     6961 ccgttcggga aatcatacac ctggtggtag atgggatgcg aaaacggcag ctcgacgaaa
     7021 tctaattcgg gaaaaacttt tttcatctgc ggacggatga actggtcgag cccgtaatta
     7081 tccgaaatgt gaaggaatcc tccgccggta aggtaattgc ggaggttgtc cgcttcctgc
     7141 tgggagaaga tcacgttgcc gtgtccggtc atgtgcacaa acgggtaatt gaaaatatcg
     7201 ggacttccca cttccacgat cgcctgctcg gggttgatgt tcgttttgat gttgtcgttg
     7261 cagaacttga tgagattcgg cagggatgtt tcgaggttgg cgtaccagtc gccgcc
//
LOCUS       DASTSF010000560         2481 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_998907,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000560 DASTSF010000000
VERSION     DASTSF010000560.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2481
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_998907"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1042
                     /locus_tag="VFU15_15890"
     CDS             <1..1042
                     /locus_tag="VFU15_15890"
                     /inference="COORDINATES: protein motif:HMM:NF024893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HEU4719326.1"
                     /translation="RMNLMGGSMLLNGSYSTQDPAVPVVDFDMNITDFDIQQTFKTFN
                     TVQKLAPVGQYTTGKFSTKLKYKSNLGPDMMPLMNTINGQGTLSTKQVEINGFAPLKK
                     LDEALKINKFSKVTMSDLNLVSYKIENGRVSTDPFDFKAGKATGKMGGSTGIDQTINY
                     VMDLAIPRDEFGPANTALNGLVSQANSKGVPVQLGDVVNVAAIFGGTVTNPTVSTNLK
                     QAGTNLMDQLKDNLTNVVTQKVDSVKNVAVNKACDEAQKQLDQAVKAAADAKAAAYKA
                     ADDAKTAAYAEADKTEKSYKNPLEKAAKKALADQMRKTADDKNKQAKDAADKAERDAD
                     AKAQADKDAKCK"
     gene            1182..>2481
                     /locus_tag="VFU15_15895"
     CDS             1182..>2481
                     /locus_tag="VFU15_15895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719327.1"
                     /translation="MKPVRYILLAAFVFVFLRPSVALAQDDDDNTSTGGDCQQIDDKE
                     AQKNFDKSKDHKKYSWDERKAFLQKALELEPDWADANMAMAKMIMNKAKADGNSDRYP
                     AAEPYLKKVCDVCPKYGAQPFYLLGTQYYLDENYPDAIIYLQKFIDYDADDPKKLGDD
                     YDFYQGQAKEMLRWSKFFVDVKKNPHPFNPHPVNYICTSMDEYLAIISPDNTMAMYIR
                     RVPVSQMDRVWGTSSTDEVFTQSFRQPDGTFDKGTRMDDPFNQNPNEGGPTLTIDNKH
                     LFYTITKDGKDGPNTDIYTTDFIDGSWTPVRSLGDKVNDPVYWDSQPSVSADGNTLYF
                     ASNRPGGQGGIDIWVTHKGFDGEWGVPENLGPTINTSGDEKSPFIHTDSQTLYFSSTG
                     FPGLGGYDIYYSRANEKGVWQTPVNMGIPINTEGDDIGLFV"
BASE COUNT          679 a          629 c          679 g          494 t
ORIGIN      
        1 caggatgaat cttatgggcg gcagcatgct gctgaacgga tcatacagca cgcaggatcc
       61 cgccgttccg gtggtggatt tcgacatgaa catcacggac ttcgacatcc agcagacgtt
      121 caaaacattc aatactgttc agaagctggc gccggtcggc caatacacga cagggaaatt
      181 ttccacgaag ctgaaataca aaagcaatct cggtcccgac atgatgccgc tgatgaacac
      241 gatcaacggg cagggaacgc tttccacgaa gcaggtggag ataaacggtt tcgcaccgct
      301 gaagaagctg gacgaagcgc tcaagatcaa taaattcagc aaggttacga tgtccgatct
      361 gaatcttgtc agctacaaga tcgaaaacgg gagagtttcc actgatccgt tcgatttcaa
      421 agcgggcaaa gcgaccggga aaatgggagg ctccacggga atcgaccaga cgatcaacta
      481 cgtgatggat cttgccattc cgcgcgatga gttcggtccg gcgaacactg cgctcaacgg
      541 acttgtttcg caggcgaatt cgaaaggcgt tcccgtacag cttggggatg tggtgaatgt
      601 cgccgctatc ttcggcggaa cggtgacgaa tcctacggtt tcaaccaatc tcaagcaggc
      661 cggcacgaat ctcatggacc agctgaagga taatcttacg aatgtcgtca cgcagaaagt
      721 ggacagcgtg aaaaatgtgg cggtgaacaa agcgtgtgat gaagcgcaga agcagctcga
      781 ccaggcggtc aaagcggcgg cagatgcaaa ggctgcggcg tacaaagctg ccgatgatgc
      841 gaaaacggcg gcgtatgccg aggcggacaa aacggaaaag agctacaaga acccgctcga
      901 gaaagcggcg aagaaggcgc tcgccgacca gatgcgcaag accgccgacg ataagaacaa
      961 acaggcgaaa gatgccgccg acaaagccga acgcgacgct gacgccaaag cgcaggcgga
     1021 taaagatgcc aaatgcaaat aacgtggtga tcggctgatt gatcaacatg cagaagtgtt
     1081 tgtttccggc cgtcggtttg tgcgtggctg catctgtgca tcagaaattt cctgtaccaa
     1141 aatggcataa ctttcgttat agctaaatca accagaatcc tatgaaaccg gtccgttaca
     1201 ttttgcttgc tgcattcgtg tttgtatttc tgcgtccttc cgtcgcattg gcgcaggatg
     1261 acgacgacaa tacctctaca ggtggcgatt gccagcagat cgacgacaaa gaggcgcaga
     1321 agaacttcga caagagcaag gatcacaaaa aatacagctg ggatgaacgc aaggcgttcc
     1381 tgcagaaagc gctggagctg gaacccgact gggcggatgc gaacatggcg atggcgaaga
     1441 tgatcatgaa caaagcgaaa gccgatggaa attcggaccg ttatcctgca gccgaacctt
     1501 acctgaaaaa agtgtgcgat gtgtgcccga aatacggcgc gcagcctttt tacctgctcg
     1561 gcacgcagta ttacctcgat gaaaattatc ccgatgcgat catctacctc cagaaattta
     1621 tcgattacga cgcggatgat ccgaaaaagc tcggcgatga ttatgatttt taccagggcc
     1681 aggcgaaaga gatgctgcgg tggtcgaagt tttttgtcga cgtgaaaaaa aatccgcacc
     1741 cgttcaatcc gcatcccgtg aattacatct gcacgtcgat ggacgaatac ctggcgatca
     1801 tttccccgga taacacgatg gcgatgtata tccgccgcgt tcccgtcagc cagatggacc
     1861 gcgtgtgggg aacttcctct accgatgaag tgtttacgca gagcttccgc cagcccgacg
     1921 gaacgttcga taaaggcacg cgcatggacg atccgttcaa ccagaacccg aatgaaggcg
     1981 ggcccacgct gacgatcgac aacaagcatc ttttttacac gatcacgaaa gacgggaaag
     2041 acgggccgaa tacggatatc tacaccaccg atttcatcga tggctcgtgg acgcccgtgc
     2101 gctcgctcgg cgataaagtg aacgatcccg tttactggga ttcgcagcct tctgtttccg
     2161 ccgatggaaa cacgctgtat tttgccagca accgtcccgg cggacagggc ggcatcgaca
     2221 tctgggtgac gcataaagga ttcgacgggg aatggggcgt tcctgaaaat ctcggtccga
     2281 cgatcaatac atccggcgat gaaaaatctc cgttcattca cacggacagc cagacgttgt
     2341 atttcagttc caccggtttt ccgggacttg gcgggtatga tatttattat tcccgcgcca
     2401 atgaaaaagg cgtgtggcag acacccgtaa acatgggaat tcccatcaac acggaaggcg
     2461 atgacatcgg gcttttcgtc a
//
LOCUS       DASTSF010000561         3324 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_506454,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000561 DASTSF010000000
VERSION     DASTSF010000561.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3324)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3324
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_506454"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1449
                     /locus_tag="VFU15_15900"
     CDS             <1..1449
                     /locus_tag="VFU15_15900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719328.1"
                     /translation="KFSGHIFCCSHGVVKRLLFLFLFLFPACRVFSQSLKVEPCAVSG
                     DTVPFMFFSNQKSAVVLKYKEVRVKCTNAGSVKIVLKGAKENYGTDTLWMNAGGSVKM
                     PPELGPHQAGVIVITYDPGGPPQRRYRIITSDKNSDALILINDDYSKPVSFSSGPVTG
                     MATAIAGRLSPADLVVRNKSDEDIFVDSIRTAPGDISVTTAFPLIIFPGSTADVPVTL
                     DLRNRLGGYFASGCAVFFHLRTKTEDESNYMFSYALVPDIGFLPNGLSLFPDNVFNAG
                     TVKRGDDAIAGFVLVNKGDRTLHTGLMPDKQITANKDSVLPGDTFHLRIALNTAYLDS
                     GSSVSRIDVAFPPYYMPVPFFIKANLLSPKPGSADALVCLSSLDFDTVCSDTAGVIKR
                     IVLLKNESKLTPLTITRVTTSDGGSMADLEGNNPVLNPGDTARVIIYFDAANRTGPYS
                     KTLSVQWDNGSLGNSPGCFLQAFKGCVIKGCR"
     gene            1565..2689
                     /locus_tag="VFU15_15905"
     CDS             1565..2689
                     /locus_tag="VFU15_15905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009581707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA0069 family radical SAM protein"
                     /protein_id="HEU4719329.1"
                     /translation="MYISYAGIHQRRRLMNMNEEEKDPGYFRGRGSQINPHNRFLKEK
                     AVQEHFEMIDEPLLLDEKTEIIYTHPKTIINRVDSPDLMGLSMNPYQGCEHGCVYCYA
                     RNTHEYWGYSAGLDFERKIIVKENAAELLQKELSNPKHVPEPIMFSGNTDCYQPVERK
                     LNITRKMLEVLLKFRHPAAMITKNSLILRDLDLLTEMAKLRLVHVMISVTGTDENMRR
                     ILEPRTATYRQRFDTIRRLDEAGVPVGVMVAPVIPGINSHEIPEVMRLAAEAGADTAG
                     MTIVRLNGQIGDIFRDWLQKNFPDRAEKVWHQVQECHGGKVNDSRFGTRMRGEGAVAE
                     SIGKLFRVSRQKYFTRDRDFEFDLSLFNPRAGDAQLSLFD"
     gene            2771..>3324
                     /locus_tag="VFU15_15910"
     CDS             2771..>3324
                     /locus_tag="VFU15_15910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719330.1"
                     /translation="MKKILSLLFLFAFCGLKAQFFAYDPFDDNSNEWFVRDDDSVTAN
                     VTGGVLDVNQKLGSFYNDVKGVTIDTAGEFRVEAMAGYVSGKTGVSYGICWGAADLGN
                     FHIFYITADGSFGFSTLHDHKLYDDFHPEQNPIINKNGKNWLRVSVEKDASGQRKMIF
                     CINELIVKTIDLIHPYGTLFGFFVG"
BASE COUNT          774 a          870 c          890 g          790 t
ORIGIN      
        1 aagttttccg ggcatatttt ttgctgttcg cacggcgtgg taaaacgcct ccttttcctg
       61 tttctgtttc tttttccggc ctgccgtgtt ttttcacagt cgctgaaagt ggaaccttgc
      121 gccgtttccg gtgatacggt cccgttcatg ttcttttcaa atcagaaaag cgctgttgtc
      181 cttaaataca aggaagtgcg tgtgaaatgc accaacgcag gttccgtgaa aattgtcctg
      241 aagggtgcaa aggaaaatta cggaaccgac accttgtgga tgaatgccgg cggcagtgta
      301 aaaatgccgc cggagcttgg cccgcaccag gcgggtgtca ttgtcattac gtacgatccc
      361 ggtggtccgc cgcagcgcag gtaccggatc atcaccagtg acaaaaacag cgatgcgctc
      421 attttgatca acgatgatta cagcaaaccg gtcagctttt cttccggacc tgtaaccggc
      481 atggcgacgg cgatagcggg acgactgtcg ccggctgatc ttgttgtgag gaacaaatcg
      541 gatgaagaca ttttcgtgga ttcgatccgc acagcgcccg gcgacatttc cgttacgact
      601 gcatttccgc tgatcatttt ccccggaagc accgcggacg ttccggtcac gctggatctt
      661 cgaaaccgcc tgggaggtta tttcgcctcc ggctgcgctg ttttttttca tctccgcacg
      721 aaaacggaag acgagtcgaa ttacatgttt tcatacgcgc ttgttcctga cattggtttt
      781 cttccgaacg gactcagcct gtttccggat aacgttttca acgccggtac cgtgaaacgg
      841 ggcgacgacg ccatcgccgg atttgtcctc gtcaacaagg gagacagaac gcttcatacg
      901 ggactcatgc ccgacaaaca gattactgcg aataaggatt ccgttctgcc cggggataca
      961 ttccatctgc gcatagcgct taacacagcg tacctcgatt ccggcagctc tgtttccagg
     1021 atcgatgttg cttttccgcc gtattacatg cccgtcccgt ttttcataaa agcaaacctc
     1081 ctctcgccga aacccggttc agcggatgcg ctcgtctgcc tttcttcact cgattttgac
     1141 acggtctgca gcgatacggc cggtgtgata aaacgcatcg tgcttctgaa aaatgagtcg
     1201 aaactcaccc cgctgaccat tacaagggtc accaccagcg acggcggatc gatggcggac
     1261 ctggaaggca acaaccccgt gctcaatccc ggcgatacag cacgagtgat tatctatttc
     1321 gatgccgcca accgcacggg tccttacagc aaaacattgt ccgtgcaatg ggacaacggc
     1381 agcctgggaa attcaccggg ctgtttcctg caggcgttca aaggttgcgt gatcaaaggg
     1441 tgccgttagc cgttaccact ttgaaacccg gaatgttgag caggtgtgtt ccgtgttttt
     1501 agcctggagt ttcgggatga attgtttccg tctcgtcatt aagccgttta cgcagctgta
     1561 aattatgtac attagctacg caggcattca tcagcggagg cgtttgatga acatgaacga
     1621 agaagaaaaa gatcccgggt atttccgcgg gcgcggttcg cagatcaatc cgcacaaccg
     1681 ttttctcaaa gaaaaagccg tacaggaaca tttcgagatg atcgatgaac cgcttctcct
     1741 cgatgagaag acggagatca tttacacaca tccgaaaacc attatcaacc gcgtcgacag
     1801 tcccgacctc atggggcttt ccatgaatcc gtaccaggga tgcgagcacg gctgcgtgta
     1861 ttgttatgcg cgcaacacgc atgaatactg gggttacagc gccggcctcg atttcgaacg
     1921 caagatcatc gtcaaggaaa atgcggcgga attgctgcag aaggaattgt caaacccgaa
     1981 gcatgtgccc gagcccatca tgttttccgg gaataccgat tgctaccagc ccgtcgaacg
     2041 gaaactgaac atcacgcgga aaatgctgga ggtgctgctg aaattccggc acccggcggc
     2101 gatgatcacg aagaactcgc tcatcctgcg cgacctcgac ctgctcactg aaatggcgaa
     2161 gctcaggctc gtgcatgtca tgatcagcgt gacaggaacg gatgagaaca tgcggagaat
     2221 tctcgagccg agaacagcca cgtacaggca acgcttcgat accatccggc gtcttgatga
     2281 agccggcgtg cctgtcggtg tgatggtcgc cccggtgatc cccgggatca acagccacga
     2341 gataccggaa gtgatgcgtc tcgccgctga agcgggcgcg gatacggccg ggatgacgat
     2401 cgtacgcctg aacgggcaga tcggtgatat tttccgcgac tggctgcaga aaaattttcc
     2461 cgaccgcgcg gaaaaagtct ggcaccaggt gcaggaatgc cacggcggga aagtgaacga
     2521 cagccgtttc ggaacgcgta tgcgcggcga aggcgctgtt gcggaaagca tcgggaaatt
     2581 gttccgcgtg tcgcggcaga aatatttcac ccgcgaccgc gatttcgaat tcgatctttc
     2641 gttgttcaac ccccgcgcag gtgacgcaca gctttcgctg ttcgactgaa cctgtggcat
     2701 gctttgtgtc cttcgcgggt aaacgggtga aagcatttat cgccatactg tataccgtta
     2761 tctttgcacg atgaaaaaaa tcctgtcact gcttttcctg tttgctttct gtggattaaa
     2821 ggcgcagttc ttcgcgtatg atccgttcga cgacaattcg aacgaatggt tcgtgcgtga
     2881 tgatgattcg gtaacggcga acgtgacggg cggtgtgttg gatgtcaacc agaagctcgg
     2941 ttctttttac aatgatgtga aaggtgtgac gatcgatacg gcgggcgaat tccgcgtgga
     3001 agccatggcc gggtacgttt ccgggaaaac gggcgtttcc tatgggatct gctggggcgc
     3061 ggcggacctc ggtaattttc acatcttcta cattacagcc gacggcagct tcggtttcag
     3121 cacgctgcac gatcataagc tctacgacga ttttcatccc gagcaaaatc ccattatcaa
     3181 taagaacggg aagaactggc tgcgcgtgag cgttgaaaaa gatgcaagcg gtcagcgtaa
     3241 aatgatcttc tgcatcaatg aactgatcgt aaaaacgatc gatctcatcc acccttacgg
     3301 aacgcttttc ggattttttg tcgg
//
LOCUS       DASTSF010000562         7374 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1338771,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000562 DASTSF010000000
VERSION     DASTSF010000562.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 7374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7374
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1338771"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1254)
                     /gene="yidC"
                     /locus_tag="VFU15_15915"
     CDS             complement(<1..1254)
                     /gene="yidC"
                     /locus_tag="VFU15_15915"
                     /inference="COORDINATES: protein motif:HMM:TIGR03593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEU4719331.1"
                     /translation="MDKKSLTGFILIALIFLAWSIYNSKEKEKEAAAEKHYTDSVFTA
                     DSLHSLTEQKQKKHAQDSIAALSRNAAPVNKDSLVKAKETAVFGCFSKSVFGDNKPVV
                     IENEKLKAWIYPRGGIIGQVELKGQKTSDGKPLILFCDDSTHFGLGFFDQQRRRLRTD
                     SLYFNVYGSGFSVKGAEHNSVTLRLYTDDDKGYIEYCYTLKGNSNLVGCTVSFRNMNT
                     VFPDDMHTVDLNWVMKAPSQEMSIKNQRAISTVFYNFDGEDGVESLSETKDEKKDFTG
                     EPKWVSFKQQYFSSILIADRKFANSGTVEVKTPSDPFIVKNLGAVIGIPFGHTARETF
                     PIKMYFGPNKFSELSSYHLHLEREINLGSSFFGSINRYIFVPLFSFLGGIFPNNYGLV
                     ILLLTVIVKIVLFPIAYKSFISAAKM"
     gene            complement(1418..3460)
                     /locus_tag="VFU15_15920"
     CDS             complement(1418..3460)
                     /locus_tag="VFU15_15920"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF017464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HEU4719332.1"
                     /translation="MKTRIIVGFVAFGALFSFAVKNAFSGSSNYTAIDTKNFDTSVRP
                     QDDFYEYVNGNWIKNNPIPATESGWGNFNVLNEKSQNALLSICNDAAKGTAAKGSNMQ
                     KIGDFYASGMDSAAIEKEKFSAVKPEMDEISKLKDMNGITPLIADMHKMQVTPGFGFF
                     VMADQKDSKTNVAYIFQSGMGLPEKAFYKSDELKMFREEYKKHLTNMFGLMGDNADDA
                     QKNSDMVFNLESMLADSAMSAVEQRDIEKQYNKMSVADLSKLCPNFNWTAYFKSIGVP
                     DLKEVIVSQVSFMRQFNALLKSQPVDAWKAYIRWHFIDAVAAKLQSDVVNEHFHFYGT
                     IMSGVQKIQPRWKRVLSATDGALGEALGQIYVERNFSMEAKQKVNVMVDNLIAAFEDR
                     IKTRTWMSDPTKDKAEKKLQTILRKLGFPDKWRDYSGLNVVRGSYVRNFLNANKFDFQ
                     YNIGKLKKPVDRMEWGMTPATINAYYNPSNNEIVFPAGIMQAPFFDPNADDAVNYGAM
                     GAIIGHELTHGFDDQGSQYDEVGNMVDWWTDDDRKNFEARTKKLSDQFSSFVAIDSLN
                     LHVNGDLTLGENIADLGGLTIAYHAYMRSLTGKPEPPKIDGFTGQQRFFLSWAQGWRN
                     SQRPAALIQQVKNNPHSPAKFRVLGPLSNLAEFYDAFGVKEGDKMWRKPEDRVEIW"
     gene            3614..4381
                     /locus_tag="VFU15_15925"
     CDS             3614..4381
                     /locus_tag="VFU15_15925"
                     /inference="COORDINATES: protein motif:HMM:NF013586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEU4719333.1"
                     /translation="MKELAFILAVAFFSSCTEDNKQSAQVHSVSPAADKSDTPAPADS
                     VKSAGDKPVPELEKQLRAAGLVDVRELDSSIRVELRYSTQNNFTGADMYGDFDKCFLQ
                     PDVARKLVKAQQELHRKFPYYSLIVYDAVRPLHIQQMMWDTLKLPPGEKQKYLSNPGN
                     GSLHNYGAAVDLSIIDDNGIPLDMGTPFDFFGELAHPEKEKELLSSGALTRNQVMNRE
                     ILRSVMYSAGFFGIQTEWWHFNSCRREEAAQKYAIVP"
     gene            complement(4385..5335)
                     /locus_tag="VFU15_15930"
     CDS             complement(4385..5335)
                     /locus_tag="VFU15_15930"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HEU4719334.1"
                     /translation="MRKILFFSLIFFAAALPAQQLPEFTQFPSILWQINPAYTGTKST
                     IDARLDYRKQWMGYDGAPVTQFAGVNSRLWKGRLGVGGTLYKDETGPSRRFIYSFQAA
                     YHLKLPDVEFCAGFSMNFDKYTLDETKMTTHWTNDPLVNNTLIDFDKNKNATGGVLLY
                     NDRFHFGLAITNFIDTHADFFTTDTTGSRVNYKAHYYFTCGYNFNKNPDYVWENNIMG
                     TYVAGLPMTLDYNLRVHYKEKILAGFAWRLRDALSLQAGYVLFESVQVIYSYDFGISP
                     LRKAHSGTHELMIGYRWNFGQHKGGYRNFDDFQKQKYHIF"
     gene            complement(5352..6422)
                     /locus_tag="VFU15_15935"
     CDS             complement(5352..6422)
                     /locus_tag="VFU15_15935"
                     /inference="COORDINATES: protein motif:HMM:NF024974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HEU4719335.1"
                     /translation="MKARTAAIFLIPAIFLGQSISAQVLTAEAGTGRMICPGTTANLG
                     GSPTASGGTSPYTYHWTPSGNISSTTSSNPIANPVVPTWYFVTVTDNAGNTATDSVFI
                     NLDPIYANNAGNDTSICINSTGTLGGMNNSTSGGVSFTWSPGASLDNPNAPRPVTSAT
                     TTTTYSLTITSTASTPNCPSKSYTVTVTVNPLPVVDACCFTTINEGESTVLNATGATD
                     YNWAPSNTLSAGNMPSVTAEPVTTTQYAVWGTDVNGCTNWDTVTVIVIPSSEIVYYNT
                     FSPNGDGINDFFYIGNAGKYPNSRLEVYTRTGQLVYAKTGYDNSWDGTNYGDKLPEAT
                     YYYRFDPGDGSPPHFGNVTIVR"
     gene            complement(6537..>7374)
                     /locus_tag="VFU15_15940"
     CDS             complement(6537..>7374)
                     /locus_tag="VFU15_15940"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEU4719336.1"
                     /translation="CVTATYNTLPALDNGYWTITASTNPTSGTYNTTLYSTNYTNATG
                     AGYTVMKATTGAGPWGLNGTCVVTSTASNTQRTGMNGFSAFAVAQSAVPLPIELLSFT
                     GTAEDDYNHLIWSTASETNNNYFTLERSVDGVTYQEIGRQNGAGTTTTRQDYSMNDLN
                     PVEGFNYYRLKQTDFNGNSTYSSVISLEFHRGHMSVTNVKPNPTNGELNFDFSTPTET
                     EIRYIITDMTGRIVVDETKVVKPGVNTITTLIDEEGAGVYSLKIIEEKHGFISVTRVV
                     KY"
BASE COUNT         1588 a         1833 c         2068 g         1885 t
ORIGIN      
        1 cattttcgca gcggagatga atgatttgta cgcgataggg aagaggacga tctttacgat
       61 cacggtcagc agcaggatga caagtccgta gttgttcggg aaaataccgc cgaggaagct
      121 gaacagcgga acgaaaatat aacggttgat cgatccgaag aaagacgatc cgaggttgat
      181 ctcgcgctcg agatgaagat ggtatgagct cagctccgaa aatttgttcg gaccgaaata
      241 catttttatc gggaaggttt cacgtgctgt gtgcccgaac gggatgccga tcacggcgcc
      301 gagatttttc acgatgaacg ggtccgaagg cgttttcacc tcgaccgttc ccgagttcgc
      361 gaacttccgg tcggcgatga gaatggagga aaaatactgc tgcttgaacg aaacccattt
      421 cggctcgccg gtgaaatctt tcttctcgtc cttggtttcg ctgaggcttt ccacgccgtc
      481 ctcaccgtcg aaattgtaga atacggtgga gatcgcacgc tggttcttga tgctcatttc
      541 ctgcgacggc gctttcatca cccagttgag gtcgaccgtg tgcatatcgt cggggaaaac
      601 ggtgttcatg ttcctgaacg aaaccgtgca gcccacgagg ttggaattcc ctttcagcgt
      661 gtagcagtat tcgatgtagc ctttgtcgtc gtcggtataa aggcggagcg tgacggaatt
      721 gtgctccgca cctttcacgg aaaacccgct gccgtataca ttgaaataaa gcgaatcggt
      781 gcgcagcctc ctgcgttgct ggtcgaagaa gccgagcccg aagtgggtgg aatcgtcgca
      841 aaaaagaatg agcggcttgc cgtcggatgt tttctgtcct ttcagctcca cctgcccgat
      901 gatgccgccc cgcgggtaga tccaggcctt cagcttttca ttttcaataa cgaccggctt
      961 gttatcgcca aaaactgatt ttgagaagca tccgaagaca gcggtttctt tcgctttcac
     1021 gagggaatcc ttgtttaccg gcgccgcatt cctgctgagc gcggcgatac tgtcctgcgc
     1081 atgctttttc tgcttctgct cggtgagaga atgcagggaa tcggccgtga aaacggaatc
     1141 ggtgtaatgt ttttctgcgg cggcttcttt ttctttctcc ttcgaattat agatagacca
     1201 cgccaggaag atcagcgcga taagaatgaa accggtaagc gactttttat ccatcaagaa
     1261 ctaattgtgc aacggcggca aagatactta tttaagcggt tgggcggagt tgcgttgtgc
     1321 cacgttctta atgtcgttcg gccggagaaa atagtctgcc gggaataaaa aaaaagcccc
     1381 ggcagagccg gggccgggga tgtctcaatc cgtggtatta ccagatctcg acgcgatctt
     1441 caggtttgcg ccacatttta tcgccttctt tcacaccgaa tgcatcataa aattctgcga
     1501 ggttggagag cggacccagc acgcggaatt ttgccggtga gtgcgggttg ttcttcacct
     1561 gctggatgag cgccgccggg cgctggctgt ttctccatcc ctgtgcccag gaaaggaaga
     1621 aacgctgctg gcccgtgaag ccgtcgatct tcggaggttc gggcttgccc gtgagcgaac
     1681 gcatataagc gtggtaggcg atggtcagcc cgcccagatc ggcgatgttc tcaccgagcg
     1741 tgaggtcgcc gttcacgtgc aggttcaggc tgtcgatggc gacgaagctg ctgaactggt
     1801 cggaaagttt tttcgttctt gcttcaaaat ttttgcggtc gtcgtccgtc caccagtcca
     1861 ccatgttccc gacctcgtcg tattgtgaac cctggtcatc aaagccgtgc gtaagctcgt
     1921 ggccgatgat cgcgcccatg gcgccgtagt tcaccgcgtc gtcagcattc ggatcgaaga
     1981 acggagcctg cataatgcct gcagggaaaa cgatctcgtt gttcgacggg ttgtaatagg
     2041 cgttgatcgt tgccggcgtc attccccatt ccatgcggtc gaccggcttc ttcagcttgc
     2101 cgatgttgta ctggaaatcg aatttgttcg cgttgaggaa attcctcacg taagatccgc
     2161 gcactacgtt cagcccggaa taatctctcc atttgtcagg gaagccgagc ttgcgcagga
     2221 tcgtctgcag ctttttctct gctttgtcct tggtgggatc gctcatccac gtgcgtgtct
     2281 tgatgcgatc ttcgaaagcg gcgatgaggt tgtccaccat cacgttcact ttctgcttgg
     2341 cttccatgct gaagttgcgt tcaacgtaaa tctgtccgag cgcttcgccg agcgcgccgt
     2401 cggttgccga aagcacgcgt ttccagcggg gctgaatttt ctgcacgccg gacatgatcg
     2461 ttccgtaaaa atggaaatgt tcgttcacca cgtcggactg gagcttggct gcgacggcgt
     2521 cgatgaaatg ccagcggata taggctttcc acgcgtcgac cggctgcgat ttcagcagcg
     2581 cgttgaactg gcgcatgaaa gaaacctgcg aaacgatcac ttctttcaga tcaggaacgc
     2641 cgatcgattt gaagtaagcg gtccagttga agttcgggca aagcttcgac agatcggcaa
     2701 cggacatttt gttgtactgc ttttcgatgt cgcgctgctc gacggctgac atggcggaat
     2761 ccgcaagcat ggattcgagg ttgaacacca tgtcggaatt tttctgcgcg tcgtccgcgt
     2821 tgtcgcccat gaggccgaac atattcgtca ggtgcttttt gtattcctcg cggaacattt
     2881 taagctcgtc gcttttgtaa aaagcttttt ccgggagacc cattcccgac tggaaaatgt
     2941 aggcgacgtt ggtcttgctg tctttctggt cggccatcac aaaaaatccg aaccccgggg
     3001 tgacctgcat tttgtgcatg tcggcgataa gcggcgtgat gccgttcatg tccttcagct
     3061 tgctgatctc gtccatctcc ggttttacag cggagaactt ttctttttcg atcgctgcgg
     3121 aatccatgcc ggaagcgtag aaatcgccga tcttctgcat gttgcttcct ttcgcggctg
     3181 ttcctttggc tgcgtcgttg cagatggaaa gaagcgcgtt ctggctcttt tcattgagca
     3241 cgttgaagtt tccccagccg gattccgtgg caggaatagg attgttcttg atccagttcc
     3301 cgttcacgta ttcgtaaaaa tcatcctggg gacggacact cgtgtcgaaa tttttggtgt
     3361 cgatcgctgt gtaattgctg cttccggaaa atgcattctt cacggcaaag gagaacaatg
     3421 ccccgaaagc gacaaaacca actataattc ttgttttcat ctggagtctg ttaggtttaa
     3481 tgaagatcga aagtaagcag aaatcccgga agggaaaggc ctcttacgca ggcggctgct
     3541 gcctgtgttg aaaggcaaat atccggtgag cggaaatggc ggaaacatcc ggattacttg
     3601 gtaatttcag gcaatgaaag agctggcttt cattcttgct gtcgcttttt tttcgtcgtg
     3661 cacggaagat aataaacagt ccgcacaggt tcattctgtt tcaccggctg cagataaaag
     3721 cgatacgcct gctcccgccg attctgtaaa atcagccgga gacaaaccgg ttccggagct
     3781 cgaaaaacaa ttgcgcgcgg cgggtcttgt cgatgtgcgg gaactggatt cctccattcg
     3841 cgtcgagctg cgttattcca cgcagaacaa tttcaccggt gccgacatgt acggcgattt
     3901 cgataaatgt tttcttcagc ccgatgtggc gcggaagctt gtcaaggcgc agcaggagct
     3961 gcaccggaaa tttccgtatt actcgcttat cgtttacgat gccgtgcgtc ccctccatat
     4021 ccagcagatg atgtgggata cgctgaagct tcctccgggg gaaaagcaga aatacctgtc
     4081 caatcccggc aacggctccc tgcacaatta cggcgctgca gtcgatctca gcattatcga
     4141 cgacaacggg attccgctgg acatgggaac gccgttcgat tttttcggag agctcgcgca
     4201 cccggaaaaa gaaaaggaat tgctgtcttc aggagcgctg acgcgcaacc aggtgatgaa
     4261 ccgcgagata cttcgcagcg tgatgtacag cgccggtttt ttcggtatac agaccgaatg
     4321 gtggcatttc aattcctgca ggagagagga agcggcgcag aaatacgcga tcgttccctg
     4381 aagatcagaa aatgtggtat ttctgtttct ggaagtcgtc aaaattccgg tagccgcctt
     4441 tgtgctggcc gaaattccac cggtagccga tcatcagctc atgcgttccg ctgtgcgctt
     4501 tacgcagggg agagatgccg aaatcgtagg agtagatcac ctgcacgctt tcgaaaagaa
     4561 cataacccgc ctgcagggaa agtgcatcgc ggagacgcca tgcgaatccc gcgagaattt
     4621 tttctttgta atgcacacgc aggttgtaat cgagcgtcat cggcagcccg gcgacatacg
     4681 ttcccatgat gttgttttcc cagacgtaat cggggttctt gttgaagtta tagccgcagg
     4741 tgaaataata atgcgctttg taattcaccc ggctgcccgt cgtatcggtc gtgaagaaat
     4801 ccgcgtgcgt gtcgatgaaa ttcgtgatgg cgagaccgaa atggaaacgg tcgttgtaga
     4861 gcagcactcc gcccgttgcg tttttgtttt tgtcgaagtc gataagcgta ttattcacga
     4921 gcggatcatt cgtccagtgc gtggtcattt tcgtttcgtc aagcgtgtac ttgtcaaaat
     4981 tcatggaaaa cccggcgcag aattccacat ccggcagctt gaggtgatac gccgcctgga
     5041 aggaataaat gaaacgccgt gaagggcccg tttcatcttt gtaaagcgta ccgcccacac
     5101 cgagacgtcc cttccataac cggctgttga cgcccgcgaa ctgcgtgacc ggtgcgccat
     5161 cgtaacccat ccattgcttg cggtaatcga ggcgcgcatc gatggtgctt ttcgtcccgg
     5221 tataggcagg attgatctgc cacaggatgg aagggaactg tgtgaattcc ggcaactgct
     5281 gtgccggcaa cgcggcggca aaaaagatca gggaaaagaa aagtattttt ctcattggtc
     5341 ttgttgtcgg ttcaccttac gatcgtcacg ttgccaaaat gcgggggtga tccgtcaccc
     5401 ggatcgaaac ggtaataata agtggcctcc gggagcttgt cgccgtagtt cgtgccatcc
     5461 cagctgttgt cgtaaccggt cttggcgtaa acgagctgtc ccgtgcgcgt gtaaacttca
     5521 agacgcgagt tgggatattt tccggcgttg ccgatgtaga aaaaatcatt gatgccgtcg
     5581 ccgttcggtg aaaaagtgtt gtagtaaacg atttcgctgc tgggaataac gatcacggtg
     5641 accgtgtccc agttcgtgca gccgttgacg tctgttcccc acacggcgta ttgcgtcgtg
     5701 gtgaccggct cggcggtgac ggaaggcatg tttcccgctg acagcgtatt cgaaggcgcc
     5761 cagttgtaat cagttgcgcc cgtagcgttc agaacagtcg attcgccttc gttgatcgtg
     5821 gtgaagcagc atgcatccac aacaggcaat ggatttacgg tcacggttac cgtgtacgat
     5881 ttgctcgggc agttgggcgt ggatgccgtg ctggtgatcg tgagcgaata agttgtcgtc
     5941 gtggttgcgg aagtgaccgg tcgcggcgca ttcgggttgt cgagcgaagc cccgggagac
     6001 caggtaaagc tgaccccgcc gctggtgctg ttgttcattc cgccgagcgt gccggtggag
     6061 ttgatgcaga tcgatgtgtc attgcccgcg ttgttcgcgt aaatgggatc gaggttgatg
     6121 aatacggaat ccgttgccgt atttcccgca ttatccgtta ccgttacgaa ataccatgtg
     6181 ggaacgaccg ggttggcgat cgggttggaa gaagtggtgc tgctgatgtt gccggaaggc
     6241 gtccagtgat acgtatacgg cgaagttccc cccgatgcgg taggactgcc cccgagatta
     6301 gccgttgttc ccgggcagat catcctgccg gttcccgctt ctgcagtcag aacctgtgcg
     6361 gaaatggatt gtccaagaaa gatggccggg attaaaaaaa tagcagctgt ccttgctttc
     6421 acagtttgtt tttgaagaga tacaaatata acgtttgggg tccaatcttt agtaagtcgt
     6481 tcctgcacaa aaagaaaaag gctcccgggg gagccttttc gtgaaaatct gaaaattcaa
     6541 tatttaacaa cgcgcgtaac ggaaatgaaa ccatgttttt cctcgatgat cttcagcgaa
     6601 taaacacctg cgccttcctc gtcgatcaat gtggtgatcg tattcacacc gggcttcacc
     6661 actttcgtct cgtccaccac gatgcggccc gtcatgtcgg tgatgatgta acggatctcg
     6721 gtttcggtcg gcgtcgagaa gtcgaagttc agttccccgt tggtcgggtt gggcttgacg
     6781 ttcgtaacgg acatgtgccc gcggtggaat tcaagtgaaa tgacgctgga atacgtgctg
     6841 ttgccgttga agtccgtctg tttgagccgg taataattga atccttccac cgggttcagg
     6901 tcgttcatgg aataatcctg ccgcgtggtg gtcgtacccg cgccgttctg tcttcctatt
     6961 tcctggtaag taactccgtc caccgatcgc tcgagcgtaa agtaattgtt gttcgtttcg
     7021 gaagcggtag accagatcag gtgattgtaa tcgtcctctg cggttccggt gaatgaaagc
     7081 agctcgatcg gcagcggaac ggccgactgc gccactgcaa atgcggagaa tccgttcatg
     7141 cccgtccgtt gcgtgttcga agccgtgctc gttaccacgc aggttccgtt cagtccccag
     7201 gggcccgcgc ctgttgttgc tttcataacg gtgtagccgg cgcctgttgc gttggtgtaa
     7261 ttcgtgctgt aaagcgtagt gttgtacgtg cccgatgtgg gattggtgga agcggtgatg
     7321 gtccagtagc cgttatcgag tgcgggcagc gtattgtatg ttgcagttac gcat
//
LOCUS       DASTSF010000563         6626 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_999297,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000563 DASTSF010000000
VERSION     DASTSF010000563.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 6626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6626
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_999297"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..403)
                     /locus_tag="VFU15_15945"
     CDS             complement(<1..403)
                     /locus_tag="VFU15_15945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719337.1"
                     /translation="MRTRAWVFLLFLFTGAGVFFGIRAFWTGLAVTDFHALTNYRDHA
                     NTITVDLSDAVYRGIEIPTVRQLPGGKFVFSFNVKNSNFSKRKFYYKIYYQDDSYKFP
                     EYSEAGGMKYENRLSSRNFYGSWGETEESFRP"
     gene            complement(546..1772)
                     /locus_tag="VFU15_15950"
     CDS             complement(546..1772)
                     /locus_tag="VFU15_15950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018631277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HEU4719338.1"
                     /translation="MRPEDLDIKNFSYDLPDEKIARFPLEKRDDSKLLVYRDGKISES
                     VFRNMAMLIPAESLLVFNSTKVVHARLLMQRKSGATVEIFCTDSGETDMDFPRLLGKT
                     GSVTILAFIGNGKRWKEDERLGATLKIGEKSFALEALRAGQKEDQFLVHLSWNDADIP
                     FLEILGAAGKVPLPPYLGRDEEENDRSRYQTVYARTEGSVAAPTAGLHFTEHVLDELR
                     RKNVHLAEVTLHVGAGTFRPVKAATMAGHEMHREQVFIPEALVEMLCDKKMKPVIAVG
                     TTSLRTLESIYWFGRQLVLQPGTFRNELFVSQWEPYEEGPDVPAEIALRAVRDWMTGN
                     NHETLEGYTKLLIAPGYRFRIADALITNFHQPQSTLLLLVAAFTGDDFRRIYDYALQH
                     DFRFLSYGDSSLLWRK"
     gene            1942..3306
                     /locus_tag="VFU15_15955"
     CDS             1942..3306
                     /locus_tag="VFU15_15955"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HEU4719339.1"
                     /translation="MNLFTKTFLAAATIVSLDAHSQCSASYTWSQSQNNVIDFANTSQ
                     PIVPNSTIFYWDFGDQQYAYTQNASHTYSGPGTYVACLYMYDSLTFCQSSYCDTVSVY
                     GNVLCNLSVNAFVVQNSSCSTCNDGVLEASASAGTPPYTYMWSNSATGAYDYNLPPGT
                     YSVCVTDANGCQSCASVTLTASSSSSCQANYIYNQSTADTVDFMNNSLGTTVGTSYYY
                     DFGDGNYSTLQNPTHAYTYPGTYNVCLYISDSANQCSSYYCASVNVWGPNSGPLCSAN
                     FVVIPDSSNSQQAWAYNYSTGSPTMQYYWTWGDNTAPDTIAYPSHVYSQTGTYTICLM
                     VYDAINQCSDTMCQTINVFRLAPEAMSLPYYVNVLPTGIHEQEQTQWSLYPNPASDEI
                     RIRTDYALQGKAYRIVDISGRVSDQGILDGNIIRVSSLENGIYFLQLDNGKGGFTVQR
                     FVKQ"
     gene            complement(3429..3836)
                     /locus_tag="VFU15_15960"
     CDS             complement(3429..3836)
                     /locus_tag="VFU15_15960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014682392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HEU4719340.1"
                     /translation="MNRPIRVLVAKVGLDGHDRGAKVIASFLRDAGMEVIYTGLRQTP
                     EMVVNAALQEDVDAIGVSILSGAHMTVFPKILSLLKEKGMNDVLLTGGGIIPDADMKK
                     LNELGVGKLFPPGTDTSEVVNYITEYVKTHRNF"
     gene            complement(4140..5333)
                     /locus_tag="VFU15_15965"
     CDS             complement(4140..5333)
                     /locus_tag="VFU15_15965"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU4719341.1"
                     /translation="MLRIALKDLRLFIKDRRAVVLTFFMPVALISIFAMAYGGEKEHN
                     AHPISLLVSDLDQTDLSRSVVDQVDSNKNIEVKRLPVDSAERLVKTGEESAVLVFAKG
                     FADSVREGKNAPVEMRYDPSKGPEVGILQQALAGSLAQIIMKSMTSGSSPQQAPVMDT
                     SMEAKIRQVISEQAEKHSGQGLKMTALIEQKQNNPGLIHAVAGTAVMMLLFGLVAMGA
                     GLLEEKENGTLKKLLYSPVSPNQILFGKMLASNVVAMVQLTVMFIYASYMFRLDLTMN
                     TPALIVMMLTTSFACSGFGMFLASVAKSRQQVQMLSTLFVLSMSAIGGSMVPSFMMPA
                     FMQKISVLSVNYWSIQGFYDIYWRNLAFNDPVFLMRPIMLVVIGAGMITLSMIFFRRN
                     VMKLS"
     gene            complement(5349..6275)
                     /locus_tag="VFU15_15970"
     CDS             complement(5349..6275)
                     /locus_tag="VFU15_15970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU4719342.1"
                     /translation="MIAVQNLKKTFGTHVALNGISFSIKEGEFYGLLGPNGAGKTTTI
                     SIISTILQPDEGSVSIGNYDLKRDPGSCKRIIGVVPQEIALYEKLTAHENLVFWGSLY
                     GGEKSRLMKRIDEILHLFGLYERRNDKISTYSGGMKRRINIASALLHNPQILFMDEPT
                     VGIDPQSRNLIFEVVEKLHSDGMTIIYTTHYMEEAERFCDRIGIIDKGEIIAQGTLQE
                     LRDQSGMKEVIVVRFASLDEEKEKMLRVKLPGEYTIANATLSYPSDNVKSDLPKIVIL
                     CGDLGLELSGIDIQKVNLETIFLRLTGKQLRD"
     gene            complement(6386..>6626)
                     /locus_tag="VFU15_15975"
     CDS             complement(6386..>6626)
                     /locus_tag="VFU15_15975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719343.1"
                     /translation="LSGAGGFISISHTRLQGGGIYIPLAVEYIPALNRSHCLSFNVET
                     DAMFTLRGTRFSGALRPLFCVAWMFTREDWNTKSR"
BASE COUNT         1515 a         1818 c         1758 g         1535 t
ORIGIN      
        1 tgggacggaa actttcctcc gtttcacccc acgatccgta aaagttccgg gacgacaacc
       61 ggttttcgta tttcattcca ccggcttcgc tgtattccgg gaatttgtag gaatcgtcct
      121 ggtaataaat tttgtagtag aatttccgct tgctgaaatt gctgttcttc acgttgaagg
      181 aaaaaacgaa ttttccaccc gggagctggc gcacggttgg aatttcaatg ccgcggtata
      241 ccgcatcgga aagatcgacg gtaatggtgt tcgcgtgatc gcggtaattg gtgagcgcat
      301 ggaaatcggt gacggcaagt cctgtccaga acgcacggat tccgaaaaaa acaccggcgc
      361 cggtgaagag aaaaagcagg aaaacccagg ctcgcgtccg catagaaaat tgcacacgaa
      421 gttagggaaa tcgggggttt gtacgcccgg cattccaatc atcgggccag gatgccaatg
      481 acagaggaaa cagaaaccgg cgcgccgtga taaaaaaatg gatactggct gtaattgcgt
      541 tagcgtcatt tcctccacag caggctgctg tcgccatagc tgagaaaacg gaaatcgtgc
      601 tgcaatgcgt aatcataaat cctgcggaaa tcatcaccgg tgaaagccgc gacaaggagc
      661 agcagcgtgc tttgcggctg gtggaaattc gtgatgagcg cgtcggcgat gcggaaccgg
      721 tatcctggcg cgatgagcag cttcgtgtag ccttcgagcg tttcatggtt gttcccggtc
      781 atccagtcgc gcacggcccg cagcgcaatt tcagccggga catccggacc ttcttcatac
      841 ggttcccatt gcgaaacgaa gagctcgttg cggaatgttc cgggctgcag tacgagctgc
      901 cttccgaacc agtaaatgct ttccagcgtg cgcagcgacg tcgttcccac ggcgatcacg
      961 ggtttcattt ttttatcgca cagcatttcc acgagcgctt ccggaatgaa aacctgctcg
     1021 cggtgcattt cgtgacccgc catggtggct gctttcacgg gcctgaacgt tcccgcgccc
     1081 acgtgcaatg tcacttccgc gaggtgcacg ttcttcctgc gcaattcgtc caggacatgt
     1141 tccgtgaaat gcaggcccgc cgtcggcgcg gcaactgatc cttccgtgcg tgcgtaaacc
     1201 gtctggtaac gcgaccggtc gttctcttcc tcatcgcggc cgagatacgg aggcagcgga
     1261 actttcccgg ccgccccgag aatttccaaa aacgggatat ccgcatcgtt ccaggaaaga
     1321 tgaacgagga actggtcttc tttctgcccc gcacggagcg cctccagcgc gaaagatttt
     1381 tcaccgattt tcagcgtcgc acccaacctt tcgtcctcct tccatctttt gccgttgccg
     1441 atgaacgcaa ggatggtgac actgccggtt ttgcccagca ggcgtggaaa atccatatct
     1501 gtttcgccgg aatccgtaca gaagatctcc accgtggccc cgcttttccg ttgcatgagc
     1561 agccgtgcat gcaccacttt cgttgagttg aaaacgagca ggctttccgc agggataagc
     1621 atcgccatat tcctgaaaac agattccgaa atctttccgt cgcggtacac cagcagcttc
     1681 gaatcgtcgc gtttctccaa tggaaagcgt gcgatctttt catcgggcag atcgtaggag
     1741 aaatttttta tgtcgaggtc ttccggtctc atgcaatggt gcaaaagtac cttttcggcg
     1801 gttttggttt tcaaccttcc gctatgcgtg actttttacg cgctgggcgg gtaagaaaac
     1861 aaggtattgg gtttaacttt tccggcaggc gcgcgtaaca ttgccatgaa atcaatgctc
     1921 aaccatttaa ccaaaccgta tatgaacctc tttacaaaaa catttctcgc tgccgcaacg
     1981 atcgtcagcc tcgacgcgca cagccagtgc tcggccagct atacatggtc gcaatcgcag
     2041 aacaacgtga tcgacttcgc gaacacatca caacccattg tgccgaactc cactattttc
     2101 tactgggatt tcggcgacca gcaatacgcg tatacacaga acgcatcgca tacatacagc
     2161 ggacccggca cgtacgtcgc ctgcctgtat atgtacgatt cactcacgtt ctgccagtct
     2221 tcctattgcg atacagtaag cgtgtacggc aacgtgctct gcaacctttc agtcaacgca
     2281 tttgttgtgc agaactccag ctgctccact tgcaatgacg gcgtactcga agcttccgca
     2341 agcgcgggaa cgccgccgta tacctacatg tggagcaatt ccgcaaccgg cgcgtatgat
     2401 tacaacctgc ccccgggaac ttactcggtt tgcgtcactg atgcgaacgg ctgccagtcg
     2461 tgcgccagcg tcacgctgac agcatcatcg tcgtcgtcct gccaggcgaa ttacatctac
     2521 aaccagtcga cggcagacac ggtagacttc atgaacaatt ccctcggcac cactgtcggc
     2581 acgagttatt attatgattt cggcgacggg aattattcca ctttgcagaa cccgacacac
     2641 gcgtacactt acccgggcac atacaacgtc tgcctttaca tttccgattc cgcgaaccag
     2701 tgttccagct attactgtgc ttcggtaaac gtctggggac cgaattccgg gcccctgtgt
     2761 tctgcgaact tcgttgtgat ccctgactct tcaaactcgc agcaggcgtg ggcgtacaat
     2821 tattccaccg gctctccgac catgcagtat tactggacgt ggggcgataa tacagcgccg
     2881 gacacgatcg cgtacccgtc gcacgtgtat tcgcaaacgg gaacctacac gatctgcctc
     2941 atggtgtacg atgccattaa ccagtgttca gataccatgt gccagacgat aaacgtattc
     3001 cgcctcgcgc cggaagcaat gtcgctgcca tattacgtga acgttctgcc caccggaatt
     3061 cacgagcagg aacaaacaca atggtcgctg tacccgaacc cggcgagcga cgagatccgc
     3121 atccgtacag attacgcttt acaggggaaa gcataccgca tcgtggatat cagcggaaga
     3181 gtttcagacc agggtatcct tgacggaaat atcatcaggg tgtccagcct cgagaacggc
     3241 atttacttcc tgcagctcga caacggcaaa ggaggattta ccgtgcaacg cttcgtcaag
     3301 caataattcc gtcaactggt ttttgaagcg acgtcccgga aattccgggg cgttgtctaa
     3361 gcgccggccg catgatcagg gtcggcgctt tttatttgcg aagcgaggtg cggacacctg
     3421 gctccacgtc agaaattgcg atgggttttc acgtactccg tgatgtaatt caccacttcg
     3481 gatgtatcgg tgccgggagg aaaaagtttt ccgacgccca gctcgttgag ctttttcata
     3541 tctgcatcgg gaatgatgcc gccgccggtg aggagcacat cgttcatccc tttttctttc
     3601 aggagcgaga ggattttcgg gaacaccgtc atgtgcgcgc cggagaggat gctcacgccg
     3661 atcgcgtcga cgtcttcctg caacgcggca ttgacgacca tttcgggtgt ctggcgcagc
     3721 ccggtgtaaa taacttccat tcccgcatcg cgcaggaagg aggcgatcac tttcgctccg
     3781 cggtcgtggc cgtcgaggcc gactttcgca acaagaacac ggataggacg gttcatgggt
     3841 gcgaaggtaa gaatgaatga tcaatgatgg atagtcagac ttcggtacgc cgcaaggcgc
     3901 ggctactcag tctgactatg agaaccgcct ttccatggga aacccattcc gccgcacgcc
     3961 agactgagta gtcacgcgta agcgggacgt accgaaggat gacaccacaa tttcaccgcg
     4021 cagtcagacg gagtagtcac gcgtaagcag gacgtaccaa agaatgactg cacaatttca
     4081 ccgcgcagtc agactgagta gtcacgcgta agcgggacgt atcgaaggat gacaccacat
     4141 cacgaaagct tcatcacatt ccgcctgaaa aagatcatgc tcagcgtgat cattcctgca
     4201 ccgataacca cgagcatgat cgggcgcatg aggaatacag gatcgttgaa cgcgaggttg
     4261 cgccagtaga tatcataaaa gccctggatg gaccagtagt tcaccgacag cacggaaatt
     4321 ttctgcatga atgcgggcat cataaacgaa ggcaccatgc ttccgccgat cgcagacatg
     4381 gaaagcacga acagcgtcga cagcatttgc acctgctgcc ggcttttcgc cacagaggca
     4441 aggaacattc cgaagcccga gcacgcgaac gacgtggtga gcatcattac gatcagcgcg
     4501 ggcgtgttca tcgtgaggtc gagacggaac atgtatgacg cataaatgaa catcaccgtc
     4561 agctgcacca tcgccacgac atttgaagca agcattttcc cgaacaggat ctggttcggg
     4621 ctcaccggcg aataaagcag ctttttcagc gtgccgttct ctttttcttc cagcaatcct
     4681 gcgcccatgg cgacaagacc aaagagcagc atcatcaccg cggttccggc aaccgcatgg
     4741 atcaacccgg gattgttctg tttctgctcg atgagcgctg tcattttcag tccctggccg
     4801 gaatgctttt ccgcctgctc gctgatcacc tggcggatct tcgcctccat cgacgtgtcc
     4861 attaccggcg cctgctgcgg cgatgatccg gatgtcatgc ttttcataat gatctgtgca
     4921 aggcttcctg cgagcgcctg ctgcaggatt cccacctcgg gtcctttcga aggatcatac
     4981 cgcatttcaa caggcgcatt tttcccttcg cggacagaat cggcgaaacc ttttgcgaag
     5041 acaagcaccg cgctttcctc tcccgttttc acgagacgtt ccgcagaatc caccgggagg
     5101 cgtttcactt ctatgttttt gttcgaatcg acctggtcga cgacgctgcg gctgaggtcc
     5161 gtctggtcga ggtcgctgac gagaagggaa atgggatgcg cgttgtgctc tttttcacct
     5221 ccgtatgcca tagcaaaaat ggagatgagc gctaccggca tgaagaatgt cagcacgacg
     5281 gcgcggcggt ccttgataaa gagacggaga tctttgagtg cgatgcgaag catgagcggt
     5341 ggaatttttt agtcgcgtaa ttgtttgccg gtaaggcgga ggaagatcgt ttcgaggttc
     5401 actttctgga tatcgatgcc ggaaagctcg aggccgaggt cgccgcacag gatgacgatc
     5461 ttcgggagat cgctcttgac attgtcgctc gggtaagaaa gcgtcgcgtt ggcgatcgta
     5521 tactcgccgg gcagctttac gcggagcatt ttttcttttt cttcatcgag cgaagcaaag
     5581 cgcacgacga tcacttcttt catcccgctc tggtcgcgca gctcctgcag cgtaccctgc
     5641 gcgatgatct cgcctttgtc gatgatgccg atgcgatcgc agaaacgttc cgcctcttcc
     5701 atgtagtgcg tggtgtagat gatcgtcatt ccgtcgctgt gcagcttttc caccacttcg
     5761 aaaatcaggt tacggctttg cggatcgatg ccgaccgtcg gttcgtccat gaacaggatc
     5821 tgcgggttgt gcaggagtgc ggaagcgatg ttgatcctgc gtttcattcc gccggaatag
     5881 gtgctgatct tgtcgttccg tctttcatac aaaccgaaaa gatgaagtat ttcgtcaatg
     5941 cgtttcatca gccgggattt ctcgccgccg tagagcgatc cccagaaaac caggttttca
     6001 tgcgcagtga gcttttcata cagcgcgatt tcctgcggca cgacgccgat gatccgtttg
     6061 cagctgcccg gatcgcgttt caggtcgtaa ttaccaatgg aaactgaacc ttcatccggc
     6121 tggagaatgg tgctgatgat gctgatcgtg gtggttttcc cggcgccgtt cggaccaagc
     6181 agtccgtaaa actcaccttc cttaatggaa aagctgatcc cgttcagcgc gacgtgcgtg
     6241 ccgaaggttt tctttaaatt ctgtactgcg atcatgaaaa ttggaattga accgctaagg
     6301 tagaaaagtt gggatgcgaa gctgaaagga aagaaaaatt ggccgcatcc cggattgcta
     6361 ttgggaaaaa tgggaaatcc tgagcttacc tgcttttcgt gttccagtct tcgcgcgtga
     6421 acatccacgc cacgcagaaa agcgggcgca gcgccccgga gaagcgtgtt ccgcgcaggg
     6481 tgaacatggc gtccgtttcc acgttaaagg aaagacaatg ggaacgattc agcgcgggaa
     6541 tgtattccac ggcgagcgga atgtatatgc ccccgccctg gagccgggtg tgggaaatgc
     6601 tgatgaatcc gcccgccccg ctgagc
//
LOCUS       DASTSF010000564         2480 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1268427,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000564 DASTSF010000000
VERSION     DASTSF010000564.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2480
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1268427"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..374)
                     /locus_tag="VFU15_15980"
     CDS             complement(<1..374)
                     /locus_tag="VFU15_15980"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEU4719344.1"
                     /translation="MKLSIKKILVPTDFSETAMLALQHAAFLARLSKAEILIAHIIPP
                     DDYHFEIPEPIMHIDNREQINKLVEQKLAETAENVKVNYGISVNTMSATGKIANEIMQ
                     IARDEKTDIIIMGTHGAKGFEEL"
     gene            complement(501..1385)
                     /locus_tag="VFU15_15985"
     CDS             complement(501..1385)
                     /locus_tag="VFU15_15985"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEU4719345.1"
                     /translation="MSQAPNRIVVPVDFGEQSMIALEQAVNVAKVIHGEITLLYVLEN
                     HGFLSRVSATQDEELRKEINQKLDELIDKLDRENKIKADKLVAQGAVYDKVAEVAEML
                     NARFIIMGTNGASSTGIRRRFIGSNALRVVRESKVPVITIKGKHHRDGCKNIVLPLDL
                     TKETREKVSKAIEIAKMYGSDIRIASVLFTTDEFIVNRLTRQLSQVKAFIEKAGVRCT
                     AEIIKGIKGEESLGQIIIDYSGKVEGDLIMIMTQQETEMSELFVGSAAQEIINKSDIP
                     VLSIVPQPKKDLSEFLPY"
     gene            1555..2430
                     /locus_tag="VFU15_15990"
     CDS             1555..2430
                     /locus_tag="VFU15_15990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019973780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HEU4719346.1"
                     /translation="MSAPIAESELILNKDGSIFHLHLLPEHLAENVILVGDQRRVETV
                     SAFFDTIEVKRSNREFITHTGTFRGKRFSVISTGIGTDNIDIVLNELDALVNIDLGTR
                     LVRPARTKLNLVRIGTSGALQGDIPVDSFVASSHGLGFDGLLNYYAGLDLCDQPSIAK
                     AFMEHAGWNKGMPYPYVVQASEFLLNKIAGGLTSGITATAPGFYGPQGRQLRLAPAMP
                     DLNEKLGSFRYEDLRITNFEMETSALYGLGKLLGHECLTVCAIIANRVARQYSKDYKP
                     VIKSLVGLVLERLAV"
BASE COUNT          578 a          650 c          611 g          641 t
ORIGIN      
        1 agttcttcga acccttttgc accgtgcgtg cccatgatga tgatatccgt tttttcatcg
       61 cgcgcgatct gcatgatctc gttggcgatt ttcccggttg cactcatcgt attcacactg
      121 atgccgtaat tcacttttac gttctccgcc gtttcggcca gcttctgttc cacaagtttg
      181 ttgatctgtt cgcggttatc aatgtgcatg atcggttccg ggatttcgaa atgataatca
      241 tccgggggaa tgatatgcgc gattaatatt tcagctttgg aaagccgggc caggaatgcc
      301 gcatgctgca aagccagcat tgccgtttcc gaaaaatcgg tgggcaccag gattttttta
      361 atggagagtt tcatagcggg taatttttat ttgacaaaat aacgattcaa tttccgtaat
      421 caaactgact ttattcattc ttcgcgctga ttgtgcgcac aaaaaagccc cgtgaccggg
      481 gctgaaggat gcgaaaaaaa tcagtacgga agaaattcag aaaggtcttt cttcggctgc
      541 ggaacgatgg acagcaccgg gatgtccgac ttgttgatga tctcctgtgc agcggagccg
      601 acaaaaagtt ccgacatctc tgtttcctgc tgcgtcatga tcatgataag atcgccttcc
      661 actttgccgg aataatcgat gatgatctgc ccgagcgact cttctccttt aatgcctttg
      721 atgatctcgg cggtgcagcg cactcccgcc ttctcgatga aagctttcac ctgcgaaagc
      781 tggcgcgtaa gcctgttcac gatgaattcg tccgttgtga aaagaacgga agctatgcgg
      841 atatcggatc cgtacatctt ggcgatctcg atcgctttcg aaactttctc gcgtgtttcc
      901 ttggtaaggt cgagcgggag aacaatgttc ttgcagccat cgcggtggtg cttgcccttg
      961 atcgtaatga cgggaacttt cgattcgcgc acaacacgca gcgcgttgga gccgatgaac
     1021 cggcgacgga tgcccgtgct cgacgcacca ttcgtaccca tgatgatgaa gcgggcgttc
     1081 agcatttccg ccacctcggc cactttatca taaaccgcac cttgcgcaac cagcttatcc
     1141 gccttgatct tgttttccct gtcaagcttg tcgatcagct catccagctt ctggttgatc
     1201 tccttgcgga gctcttcgtc ctgcgtagcg ctcaccctgc ttaaaaagcc atgattttca
     1261 agaacataca gaagtgtaat ttctccatga ataacttttg caacatttac agcctgttca
     1321 agggcgatca tggattgttc tccgaaatct acgggaacta caattcggtt gggtgcttga
     1381 ctcataggtt tttaatttcg gtcagtttga tgggtcgtgg tttcaaaaaa gttatgcaaa
     1441 attagcacaa aattcgatcc gtgaaagcag ggttttcccg ctgcaaccgc ttataaatcc
     1501 ggaattcccg gggccgcctg aaaaaaaatc cctttttttg aaaaaataaa catgatgagc
     1561 gcacccattg ccgaatcgga actgatcctg aacaaggacg gaagcatttt ccatctccac
     1621 ctgctgccgg aacacctggc cgaaaacgtt attctcgtgg gcgaccagag aagggtcgaa
     1681 actgtttcgg ctttctttga cacgatcgaa gtgaaacgca gcaaccggga attcatcacc
     1741 cacacgggaa cgttccgcgg aaagcgtttt tccgtcatct ccacaggcat cggaaccgac
     1801 aacatcgaca ttgtcctgaa tgagctcgac gcgctcgtga acatcgatct cggcacccgc
     1861 ctcgtcaggc ccgcgcgcac taagcttaac ctggtgcgga tcggtacgtc cggggcgctg
     1921 cagggcgata tcccggtgga ttcattcgtc gcttcttccc acggactcgg gttcgacggg
     1981 cttctcaatt attacgccgg gctcgacctg tgcgaccaac cttccattgc gaaggcgttc
     2041 atggaacatg ccggatggaa taaaggaatg ccctatcctt acgtcgtgca ggcttctgaa
     2101 ttcctgctga acaaaattgc cgggggactc accagcggga taaccgcgac ggcgcccggt
     2161 ttttatggtc cgcagggaag gcagcttcgt cttgcgcctg ccatgccgga cctcaatgaa
     2221 aagctcggct ccttccgtta tgaagacctc cgcatcacga atttcgaaat ggaaacctcg
     2281 gcgttgtacg ggctcggaaa attgctcggt catgaatgcc tgaccgtatg cgccatcatc
     2341 gccaaccgcg tggcaaggca atacagcaaa gactacaaac cggtcataaa aagccttgtg
     2401 ggcctcgtcc ttgaacgtct ggctgtgtaa aagtcggttg ggcgccacaa caaaaagcgt
     2461 acgattggcg tatatttgat
//
LOCUS       DASTSF010000565         5958 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1894379,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000565 DASTSF010000000
VERSION     DASTSF010000565.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5958)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5958)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5958
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1894379"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1013
                     /locus_tag="VFU15_15995"
     CDS             <1..1013
                     /locus_tag="VFU15_15995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008505981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEU4719347.1"
                     /translation="LYTFLIATFEILAVLVFLGCFVFFCRRNFLKLKRLASKDLDGWP
                     RSDANYILITEMALMTAFLFMNAADVRLQAMHFSHYDIGLSQAAFPVSRFLSSLYGDA
                     SSASLVMAERFTWWFHITGIMAFLNYLPYSKHFHIILAFPNTFFSNLKPKGEFTNLAS
                     VTTEVKAMLDPSFTPPPPPATPQRFGAKDVQDLTWKQLMDAYSCTECGRCTSSCPANI
                     TGKKLSPRVIMMKTRDRVEEVGKNRDANKGKFVDDGKSLLNDYITPEELWACTSCNAC
                     VQECPVNIDPLSIIVDMRRYLVMEESAAPAELNMMMTNIENNAAPWQFSPADRLNWAN
                     EQ"
     gene            1016..1393
                     /locus_tag="VFU15_16000"
     CDS             1016..1393
                     /locus_tag="VFU15_16000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719348.1"
                     /translation="MKRLAFAAIIILLLASMKQEQKTAYSVELFPLNDTLVNGIDNTM
                     TILKNGKPAGEIILMMSGASVAKKGQDYVARTDMSMIGKESKLQVKERQKVVLNKSFP
                     VVELKGDLVQRVAEKMKQSDQSK"
     gene            1395..1823
                     /locus_tag="VFU15_16005"
     CDS             1395..1823
                     /locus_tag="VFU15_16005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008240458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoheptose isomerase"
                     /protein_id="HEU4719349.1"
                     /translation="MSVGKDLLKEVKENGQKISPVLPENVKNFLIDIDGTICDDIPNE
                     EPWRMRDAGLYPGALETISKWYDEGHIITFFTSRTEEHRDVTEEWLLKNGFRWHGLLM
                     GKPRGGNYHWIDNHMVRATRYSGKFTDLVEKEVTIQVFES"
     gene            1835..2629
                     /locus_tag="VFU15_16010"
     CDS             1835..2629
                     /locus_tag="VFU15_16010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020211521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEU4719350.1"
                     /translation="MSNEPIHVPTMAEMASKGETPEILFWVGCAGSFDDRAKKITKAI
                     VRILNHVGMKYAVLGTEETCTGDPAKRAGNEFLFQMQAMNNITVLNGYGVKKIVTGCP
                     HCFNTLKNEYPGLGGTYEVIHHTQLVQQLIDAGKLKVQGGEFKGKKITFHDPCYLGRA
                     NNEYEAPRSVIEKLDVELAEMKRSRQNGLCCGAGGAQMFKEAEKGEKEVNVERSEEAL
                     ALNPDIIATGCPFCMTMMTDGVKHFNREDKTKVLDVAELIAKANEL"
     gene            complement(2774..3391)
                     /locus_tag="VFU15_16015"
     CDS             complement(2774..3391)
                     /locus_tag="VFU15_16015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719351.1"
                     /translation="MNKAPLLFISLVLAVIISCNSREKKERNDSSAVKADGPVPVNYL
                     FDSLPGSVFYIGQMDTSNCRVSYGVDSGVMLAFIDDSTVVRSGDFMSDRDIFTGHYRV
                     ESDTLVIRYDSTYASRQCGDQKNGNTVTIGGDVKNKILTRQLSPVKIVVRKCSSGRRY
                     LSTAKKKSDYLDQSEGDAVRDSTGAGEIIKKLEADSLWQLLFPGK"
     gene            complement(3397..4095)
                     /locus_tag="VFU15_16020"
     CDS             complement(3397..4095)
                     /locus_tag="VFU15_16020"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEU4719352.1"
                     /translation="MKKQLLFFCLILLSLSASAQQFHAGLTGGLTVTDIPGMDPRDPD
                     EDFNKLGFVAGGIVSTILSDQNDFQFELNFIQKGSAQRPDSLNNGQFSLALNYVEVPF
                     VFRHRMNINLKQKTVRRLGFECGLSLGRLVHERMLANNYTQTLSATDFNYTDISLLAG
                     LDYLFTDHFMFSLRYSNSVIPAVKRNAIMPGFVRYSFNQGNNMVFQLSFKYIFGKATA
                     IQDTNNQPPPTDNQ"
     gene            complement(4172..4588)
                     /gene="ruvX"
                     /locus_tag="VFU15_16025"
     CDS             complement(4172..4588)
                     /gene="ruvX"
                     /locus_tag="VFU15_16025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007806871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEU4719353.1"
                     /translation="MPRILAIDYGQKRCGIAVTDTQQIIATGLTTVQTHELMKFLEDY
                     FQKETVETVVVGEPKKMSGESTHATPLVAAFVKNFRKHFPHIPVTTIDERFTSKIAFR
                     SMVDSGLKKKDRANKELIDEVSATILLQDYLSGSRS"
     gene            4653..5468
                     /locus_tag="VFU15_16030"
     CDS             4653..5468
                     /locus_tag="VFU15_16030"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017498319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HEU4719354.1"
                     /translation="MENLQNTIEKAWEDRSLLNDASVQQSIAGVIELLDKGKLRVAEP
                     SGDTWKVNDWVKKAVILYFPIRKMETMEAGPMEFHDKMALKRNYAELGVRVVPHAVAR
                     YGAYLAKGVIMMPSYVNIGAYVGSGTMVDTWATVGSCAQIGKDVHLSGGVGIGGVLEP
                     VQAAPVIIEDGAFIGSRCIVVEGCRVGKEAVLGANVVLTGSTKIIDVTGEKPVEYKGF
                     VPARSVVIPGSYAKKFAAGEFNVPCALIIGKRKESTDLKTSLNNALREYDVAV"
     gene            5527..>5958
                     /locus_tag="VFU15_16035"
     CDS             5527..>5958
                     /locus_tag="VFU15_16035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719355.1"
                     /translation="MFAHDSYDSLFFYYYSAGISSLYPDTSQPDFPEENFIHPREENL
                     VLPGNGSCLLNNTWTDYDNTGTGFSTSREFCLVRKTKDDENLVMISFALKGKDTVVDR
                     KLECRYLFGKRPLVFVLRDFRTGYIDSSLYRYSRSGLLSGVH"
BASE COUNT         1443 a         1499 c         1677 g         1339 t
ORIGIN      
        1 cgctgtatac gttcctcatc gcgacgtttg agatcctcgc cgttctcgtt ttcctcggct
       61 gcttcgtgtt tttctgccgc aggaatttcc tgaagctgaa acggctggcc agcaaggatc
      121 tcgacgggtg gccgcgctcc gacgcgaatt acatcctcat caccgaaatg gcgctgatga
      181 cggcgttcct gttcatgaac gcagctgatg tccgccttca ggccatgcat ttcagtcatt
      241 acgatatcgg tctttcgcag gccgcgttcc cggtcagccg ttttctctcg tcgctttacg
      301 gcgatgcgtc ttcggcctcg ctcgtgatgg cggaacgttt tacgtggtgg ttccacatca
      361 ccggcatcat ggccttcctg aattacctgc cgtactcgaa gcatttccac atcatcctcg
      421 cgttcccgaa cacattcttc agcaatctca aaccgaaagg tgaattcacc aacctcgcat
      481 cggtgacgac ggaagtgaaa gccatgctcg atccttcatt cacgccaccc ccgccgcctg
      541 caacaccgca gcgtttcggg gcgaaagacg tgcaggacct gacgtggaag caattgatgg
      601 acgcctattc ctgcaccgag tgcgggagat gcacatcgtc ctgcccggcg aacatcaccg
      661 ggaaaaaatt atcgccgcgc gtgatcatga tgaaaacgcg cgaccgcgtc gaggaagtcg
      721 gaaagaacag ggacgcgaac aaaggaaaat tcgttgacga cggtaaatcg ctgctgaacg
      781 attacatcac gcccgaggaa ttatgggcgt gcacttcgtg caatgcgtgc gtgcaggaat
      841 gtcccgtgaa catcgacccg ctttccatta tcgtcgacat gcgccgttac ctcgtgatgg
      901 aggaatccgc tgcgcccgcc gaactgaaca tgatgatgac gaatatcgag aacaatgccg
      961 cgccctggca gttctcgcca gcggatcgtt tgaactgggc gaacgagcaa taaagatgaa
     1021 gcgcctcgca tttgctgcaa tcataatcct gctgctggct tcgatgaagc aggagcagaa
     1081 gaccgcctat tcagtggagc tttttccgct gaacgataca ctggtgaacg ggatcgataa
     1141 tacgatgacg atcctgaaaa acgggaagcc ggccggagag atcatcttga tgatgtccgg
     1201 cgcttcggtg gcgaagaaag gacaggacta tgtcgccagg accgatatgt cgatgatcgg
     1261 gaaagaaagc aagctgcagg taaaagagag gcagaaagtg gtgctgaata aaagcttccc
     1321 ggtcgtggag ttgaaaggcg acctcgttca gcgggtggcg gaaaaaatga aacaaagcga
     1381 tcagtcgaaa taacatgagc gtagggaaag atctgctgaa agaagtaaaa gagaacggac
     1441 aaaaaatcag tcccgttctc ccggagaacg tcaagaattt tctcatcgat atcgatggaa
     1501 cgatctgcga cgatatcccg aatgaagagc cgtggcgcat gcgcgatgca ggcctgtatc
     1561 ccggtgcgct cgaaacgatc agcaagtggt acgatgaagg acacatcatc acgttcttca
     1621 cttcacgtac cgaagagcat cgtgacgtca ccgaagagtg gttgctgaaa aacggttttc
     1681 gctggcacgg actgctgatg ggaaaaccgc gcggaggaaa ttaccactgg atcgacaatc
     1741 acatggtgcg cgcgacgcgc tattccggga aattcaccga tctcgtggaa aaagaagtaa
     1801 caatacaggt gttcgaatca taactgttac cgaaatgagc aacgaaccaa tccacgtacc
     1861 gacgatggcc gagatggctt ccaaaggtga aacgcccgag attttattct gggtgggctg
     1921 cgccggaagc ttcgacgacc gtgcgaagaa gatcacgaag gcgatcgtac gcattttgaa
     1981 tcacgtcggg atgaaatacg ccgtgctcgg tacggaagaa acctgcaccg gcgatcctgc
     2041 gaaacgtgcc ggcaacgaat tccttttcca gatgcaggca atgaataata tcacggtgct
     2101 gaacggttac ggtgtgaaga agatcgtcac gggttgcccg cattgcttca acacgctgaa
     2161 gaacgaatat cccggcctgg gcggaacgta cgaagtgatc caccacacgc agctcgtgca
     2221 gcagctcatc gatgccggaa agctgaaagt gcagggtggg gaattcaaag gaaaaaaaat
     2281 aaccttccac gatccgtgct acctcggtcg cgcaaacaat gaatacgaag cgccgcgttc
     2341 cgttattgaa aaactcgatg tggagctcgc ggaaatgaaa cgcagccggc agaacggtct
     2401 gtgctgcggc gccggcggcg cacagatgtt caaggaagcg gaaaaaggcg agaaggaagt
     2461 gaacgtggaa cgttcggaag aagcgctcgc gctgaatccc gatatcatcg caacgggttg
     2521 ccccttctgc atgacgatga tgaccgacgg cgtgaagcat tttaacaggg aagacaaaac
     2581 caaagtgctc gacgtggcgg agctgatcgc caaggcgaac gagctgtgag gttttaccgg
     2641 aaaatttccg gaggaaacgc agcgcttttc tgcgcactcg gcggttaaaa agatcaccac
     2701 aaggtttatg ggacacatca caaagcttac agcgaaaagg ctgtgtttgt ttgtgcgggt
     2761 tggggttaaa agatcatttc ccggggaaaa gcagctgcca caggctgtcc gcttccagtt
     2821 tttttatgat ttcgccggca cccgttgaat ccctcacggc gtcgccttcg gactggtcca
     2881 ggtaatcgct tttctttttc gcggtcgaaa gataacgccg gccgctgctg catttcctta
     2941 ccacgatctt caccggactc agctgccggg tgaggatctt gtttttgacg tcaccgccaa
     3001 tcgtaaccgt gttcccgttt ttttgatcgc cacattgccg gcttgcatag gtggaatcgt
     3061 accggatgac gagcgtgtcg ctttcaacac gataatgccc ggtaaaaata tcccggtccg
     3121 acataaaatc accggaacgg acgaccgtgc tgtcgtcgat gaacgcaagc atcacgccgc
     3181 tgtcgacacc gtaggaaacc cggcaattcg aagtgtccat ttgcccgatg taaaaaacgg
     3241 aacccggaag tgaatcgaag agatagttta ccggaacggg gccatcggct tttaccgcgg
     3301 acgaatcgtt ccgctctttc ttttcgcgac tgttacagga aataattacg gcaaggacaa
     3361 gtgaaatgaa aaggagaggg gctttgttca tgccgtttac tggttgtccg ttggcggcgg
     3421 ctgattgttc gtatcctgga tcgctgtcgc tttcccgaaa atatatttaa agctcagctg
     3481 gaaaaccatg ttgttgccct ggttgaagga ataacgcacg aagcccggca tgatcgcatt
     3541 ccgctttacg gcgggaatca ccgaattgct gtaacgcagc gagaacatga aatgatcggt
     3601 gaaaagataa tcgagcccgg cgagcaggct gatgtcggtg tagttgaaat cggtggcgga
     3661 gagcgtctgc gtgtaattgt tcgcgagcat gcgttcatgc acgaggcgcc cgagggaaag
     3721 tccgcactcg aagccgagcc ggcgtacggt tttctgcttc aggttgatgt tcatgcggtg
     3781 gcggaaaacg aacggcacct cgacgtaatt cagcgcgagg ctgaactggc cgttattcag
     3841 cgaatcagga cgctgcgcgc ttcctttctg gatgaagttc agctcgaact ggaaatcgtt
     3901 ctggtcgctg aggatcgtgg aaacaattcc gcctgcgacg aagccgagct tgttgaagtc
     3961 ttcatcggga tcgcgcggat ccattcccgg aatatcggtg acggtaagtc cgccggtgag
     4021 cccggcgtga aattgctgtg cgcttgctga aagcgaaagg agaatgaggc aaaaaaagag
     4081 aagctgtttt ttcatgaccg ttgaagctat ggttacgggt tttcaacgga gcgaaaagga
     4141 agaaacccgt ggcaaaagta aatctttgcg ttcagctgcg cgagccggaa agataatcct
     4201 gcaataaaat agttgcgctg acctcgtcga tcagctcctt gttcgcccgg tcttttttct
     4261 tcagcccgga atcgaccatc gaccggaaag cgatcttcga ggtgaagcgt tcgtcgatcg
     4321 ttgtcaccgg aatgtgcggg aaatgtttcc ggaaattctt cacgaatgca gccaccagcg
     4381 gcgtcgcatg cgtgctttcc ccgctcattt ttttcggttc cccgacgacg acggtttcca
     4441 cggtttcttt ctgaaaataa tcttccagga atttcatcag ctcgtgcgtc tgcacggtgg
     4501 tcagtcccgt cgcgatgatc tgctgcgtgt cggtcactgc tatgccgcag cgtttctgtc
     4561 cgtaatcgat ggcgagaatt ctcggcattc gctttccaat ttttcgtgcg aatgacgaag
     4621 ttaagtcgct tcgccctaat ttcgcagaac ttatggaaaa cctgcagaac acgattgaaa
     4681 aagcctggga agaccgttcg cttttaaatg acgcgtctgt tcagcaatcg atcgctggtg
     4741 tgatcgaact gctcgacaaa ggaaagctgc gcgtggccga gccttccggc gatacgtgga
     4801 aagtgaacga ctgggtgaag aaggctgtca ttctttattt ccccatccgc aaaatggaaa
     4861 cgatggaagc cggtccgatg gaattccatg ataagatggc gctgaaaagg aattatgcgg
     4921 agctcggcgt gcgcgtggtg ccgcatgcgg tggcgcgtta tggcgcgtac ctcgcgaagg
     4981 gcgtgatcat gatgccgtcg tatgtgaaca tcggcgcgta tgtcggcagc ggcacgatgg
     5041 tggacacgtg ggcgacggtc ggttcctgcg cgcagatcgg gaaagacgtt cacctcagcg
     5101 gcggcgtcgg gatcggcggt gtgctggaac ctgtgcaggc tgcgccggtg atcatcgaag
     5161 acggtgcgtt catcggctcg cgctgcatcg tggtggaagg ttgccgcgtg ggaaaagaag
     5221 cggtgctcgg cgcgaacgtc gtgctcacgg gctcaacgaa gatcatcgat gtcaccgggg
     5281 aaaagcccgt ggaatacaaa ggttttgttc ctgcacgttc ggtggtgatc cccggcagct
     5341 acgcgaaaaa atttgcagcg ggcgaattca acgttccctg cgcgctcatc atcggcaaac
     5401 gcaaggaaag caccgacctg aaaacgtcgc tgaacaatgc actgagagaa tatgatgtgg
     5461 cggtgtagac ataatttttt gcacgtcctg tttgcctgtg tagctttttt gttttctacc
     5521 cgggcaatgt tcgcgcatga ttcctacgat tcgctttttt tctattatta ttccgcaggg
     5581 atttcttctc tttacccgga tacttcgcaa cccgattttc ccgaagagaa ttttattcac
     5641 ccgagggaag agaacctggt gctgccggga aacggatcgt gcctgctcaa caacacctgg
     5701 accgattatg acaatacagg gaccggtttc agcacttcgc gggaattttg cctggtccgc
     5761 aaaacgaagg acgatgaaaa cctggtgatg atctctttcg ccttgaaagg aaaagatacg
     5821 gtcgtcgacc gcaagctcga atgccgttac cttttcggaa agcgaccgct tgtttttgtg
     5881 ctgagggact tcaggacagg ttacatcgat tcctcgcttt accgctattc caggtcgggt
     5941 cttctgtccg gtgttcac
//
LOCUS       DASTSF010000566         2741 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_649549,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000566 DASTSF010000000
VERSION     DASTSF010000566.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2741
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_649549"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(18..1187)
                     /gene="neuC"
                     /locus_tag="VFU15_16040"
     CDS             complement(18..1187)
                     /gene="neuC"
                     /locus_tag="VFU15_16040"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002956452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0006047 - UDP-N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HEU4719356.1"
                     /translation="MKKIKVLGVTGIRSEYDILYPLMKLLRDDPRFEVKLAVSGAHLS
                     GWHGEALQRIADDGFTIADKIDSLLMTNRKTQRVKGVGILTTALAQTVEREAPDFLYV
                     VGDREESIATAIVGNYMDVLVAHLGGGDPVFGNADDPIRFAVSRLAHLHLVTAKPYAE
                     NLAKVGEEDFRIHFVGNPAYDSIMQVPQLSPAETAARLGMDISDGKYIVVIKHPLSSE
                     KEDAYFQMKTALEGISLFCRKTGFKAVGIHPNTDPGSYDILQAISEAESPSIRFFKTL
                     DRELFVNLVRNAKALAGNSSMGILEAPFYKLPVVNIGNRQQGRLNAGNVEFVSYDPDV
                     ICNALIKAATDESYREKISKFENPYGNGGTSEKIRDILLSVDLTDKKWYVKQKLC"
     gene            complement(1184..1873)
                     /locus_tag="VFU15_16045"
     CDS             complement(1184..1873)
                     /locus_tag="VFU15_16045"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU4719357.1"
                     /translation="MEELKRDSWESSYSRRENFIYYPKEEVVKFLNRFVRKRTGTGSF
                     DDILHPGQKLAGLDFGCGIGRQVVLMEEFGIAANGVDISATALAEAEKVYEFFLGKKP
                     GEGTFRKLDGTALPFADRAFNIAVCDSVLDSMYFRLAKEIVTELDRTVKDMLFISLIA
                     PVKNGKAEDTIVTEAHEADTVQSYYDEKRIEKLLEGSAWEVRWLNLVHETVPASGFEN
                     ARYHVVLKKRS"
     gene            complement(1861..2514)
                     /locus_tag="VFU15_16050"
     CDS             complement(1861..2514)
                     /locus_tag="VFU15_16050"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEU4719358.1"
                     /translation="MKKAVFFDFDDTLYDQDLYYLAVFRTFSGMHGIDGEQMMRSYRS
                     LKGKSRDIFGGVLSEMGIYSADLQEELFRLYRTQEVSIPVYPDADEILGWLKEKNIAT
                     GIITNGVVDVQKNKVKATGMDHRVDIVVYARTWGAGFEKPHPRAFGEALERAGVSAGN
                     AIFVGDVPETDIRGAEAAGIANVFLVDRNGKFENNSGYKRISELTELKKIISEIWKN"
     gene            complement(2511..>2741)
                     /locus_tag="VFU15_16055"
     CDS             complement(2511..>2741)
                     /locus_tag="VFU15_16055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719359.1"
                     /translation="PMRGFFCIQFIEDDGAYYLTDVNPRIGGGSILSLHCSDSMRSNL
                     VAILKNEHEKIKPSIGAYREESMFRYYQEYYV"
BASE COUNT          629 a          802 c          586 g          724 t
ORIGIN      
        1 cagcaccttt tttttcatca gcatagtttt tgcttaacgt accatttttt atcggtgaga
       61 tccacggaaa gaagaatatc cctgatcttt tccgacgttc ctccattccc gtacggattt
      121 tcaaatttgc ttattttttc cctataggac tcatccgtcg ccgcttttat aagcgcattg
      181 catatcacgt caggatcata cgaaacgaat tcgacgttac cggcgttgag ccttccctgc
      241 tgcctgttcc cgatgttgac aacaggcaac ttataaaacg gcgcctcgag tattcccatg
      301 ctcgagttcc ccgccagcgc ttttgcgtta cgcacaagat tcacaaaaag ctccctgtct
      361 aacgttttga agaaccggat ggaaggactt tcggcttcag aaatagcctg aagaatatcg
      421 tacgaacccg gatccgtatt cgggtgtatt ccgaccgctt tgaacccggt cttccggcag
      481 aacagcgaaa ttccttccag cgcggttttc atctggaaat aagcatcttc tttttccgat
      541 gaaagcgggt gctttatcac cactatgtat tttccgtcgg aaatatccat tcccagccgg
      601 gccgctgttt ctgccgggga aagctgcggc acctgcataa tggaatcata cgcgggattt
      661 ccgacaaaat gaatgcggaa atcctcctct cccaccttcg ctaaattttc tgcgtacggt
      721 ttcgccgtca ccaggtgaag atgcgccagc ctcgacacgg caaaccggat gggatcatcg
      781 gcattcccga aaacagggtc gcccccgccg aggtgcgcca ccagcacatc catataattt
      841 ccaacgatcg cggtggcgat gctttcctcc ctgtcgccga caacataaag aaaatccggt
      901 gcttcacgct cgacggtttg cgccagtgcc gtggtgagaa taccgacgcc tttaacgcgc
      961 tgcgttttcc ggttggtcat cagcagcgaa tctattttat cggcgatcgt aaatccgtca
     1021 tccgcgatac gctgcagcgc ttcgccatgc cacccggaaa gatgcgcccc ggaaacagcg
     1081 agcttcacct cgaagcgggg atcatccctg agcagtttca tcagcgggta cagaatatca
     1141 tattcgctcc ggataccggt cacaccgaga actttgatct tcttcatgaa cgttttttta
     1201 gaacaacgtg atacctcgca ttttcaaacc cggaggcggg aacggtttca tggacaagat
     1261 tcagccacct gacctcccat gccgaacctt cgagcagctt ttctattctt ttttcatcgt
     1321 aatagctttg caccgtatcc gcctcgtgcg cttccgtcac gatcgtatcc tctgcttttc
     1381 cattctttac cggcgcgatc aggctgatga agagcatatc ttttacggtt cggtccaatt
     1441 ccgtcacgat ctcttttgcc agcctgaaat acatgctgtc gagcacgctg tcgcaaaccg
     1501 cgatattgaa cgcgcgatcc gcgaacggta acgcagttcc atccaatttc cggaaagtac
     1561 cttcgccggg ctttttccca aggaagaact catatacttt ttccgcctca gcgagggcag
     1621 ttgcagaaat gtcgacacca ttcgcagcaa ttccgaactc ctccataagc accacctgcc
     1681 tcccgatgcc gcatccgaaa tccagtcctg cgagtttctg tccggggtgg agaatatcat
     1741 cgaaacttcc ggtgccggtt cttttccgca cgaaacggtt gaggaatttc accacctcct
     1801 ccttgggata gtaaataaaa ttttcacggc gggagtaact gctctcccat gaatcccttt
     1861 tcagttcttc catatctcag aaatgatttt tttcaactcc gtaagttccg aaatccgttt
     1921 gtatccgctg ttattttcaa atttcccgtt cctgtcgacg agaaagacat tggctattcc
     1981 cgccgcttcc gcaccacgga tgtccgtttc cggcacatca cccacgaaaa ttgcgtttcc
     2041 ggcgctgacg cccgctctct ccagggcttc cccgaacgca cgcggatgcg gcttttcaaa
     2101 cccggcgccc caggtcctcg cataaacgac aatatcgaca cggtgatcca ttcctgttgc
     2161 ctttactttg ttcttctgca catcgaccac accgttcgta atgatccccg tggctatgtt
     2221 tttttctttc agccatccca ggatttcatc cgcatccggg taaacaggaa tcgagacctc
     2281 ctgcgtccgg tagagccgga aaagttcttc ctgcagatcc gccgaataaa ttcccatttc
     2341 gctcaacact ccgccgaaga tatcgcgcga ttttcccttc agcgaacggt aactccgcat
     2401 catctgttcc ccgtctatgc cgtgcatccc ggaaaacgtc cggaaaaccg ccaggtaata
     2461 aagatcctgg tcgtaaagcg tgtcatcgaa atcgaagaaa actgcttttt tcacacgtaa
     2521 tactcctggt aatagcggaa catgctttct tcacggtacg caccgatcga cggttttatt
     2581 ttttcatgct cgtttttcag aatagctaca aggttggacc gcatgctgtc cgaacaatgc
     2641 agcgaaagaa tgcttccgcc cccgattctc ggattcacgt ccgtgaggta ataagcacca
     2701 tcatcctcga tgaactgaat gcagaaaaat cccctcatag g
//
LOCUS       DASTSF010000567         5781 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_561075,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000567 DASTSF010000000
VERSION     DASTSF010000567.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5781
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_561075"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2096
                     /locus_tag="VFU15_16060"
     CDS             <1..2096
                     /locus_tag="VFU15_16060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719360.1"
                     /translation="FAPEMCTIFPRMWEMNQKVHVKEYESWGNVAHNHVTVRIPDRQT
                     GEMQSYEKPTMLANLTYFFRYQVNWMYMRYFFWNFVGRQNDIQGHGNLTDGNYQSGIA
                     AFDKVDSKKEPKRLRENAANNKFYYIPLILGLLGMYFHFAKDWKSALVIGLLFLFTGL
                     AIVVYLNQYPLQPRERDYAYAGSFYAFAFWIGFGVYALYDLFTERLKLQGTMAAGLAT
                     VMCFSGPYLMGQQGWDDHNRSNRYTCRDFAENYLSTCDKNAILFTNGDNDTFPLWYAQ
                     EVEGYRTDVRVLNLSLLNTDWYIDQANRKAYDSDRVPFSLPSTRYRQGSCDQVFFDPD
                     KKNKTKEMSARELVDMIIHANPDAMSVRRGEDDNGDVYPDTVFVLPTNKIYIPVDKKL
                     VKSNGTVPADTPDSLIADRVEWTINKSYLLKADLMILDLLASNNWQRPIYFAVTAGSD
                     SYLNLEGWFRLEGLAYRLVPMKADKRGIFSEPVGCNTDMMYDKIMNKFKWGGLEDKSK
                     KIYMDENNLRFTTNQRLQMMTLAKLLNDEGKHDKAKKVLDRCLEVMPEEHVPYEPAMV
                     YMVDGYYRAGAMDNANKLSKTLFDQCEEEYNFYNSLAGKTPQGGYDDDANRLASALQM
                     LSQFADKYGQKDMSADYRKRLTAMGLGAPTDQPDFGGDDGNGGMDPKLKARIDSMMNA
                     MKQDSGKKKQQPPHK"
     gene            2099..2710
                     /locus_tag="VFU15_16065"
     CDS             2099..2710
                     /locus_tag="VFU15_16065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013667385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEU4719361.1"
                     /translation="MRILARPPFLLRQLYPGAAWRMNASEKKIFLTFDDGPVPGVTPA
                     ALSVLKEQDVRATFFCVGENVQRHPEIFSSILEQGHRVGNHTFHHADGWKHTSRSYLR
                     EVRQCAEVVKSDLFRPPYGRMRRSQFEAIRRKYKVIMWDVLTCDFDLSLSCEDVLSIA
                     AGHSRPGSVIVFHDSLKAKERMLYVLPKYIAEMKTRGFAFDVL"
     gene            complement(2712..3125)
                     /locus_tag="VFU15_16070"
     CDS             complement(2712..3125)
                     /locus_tag="VFU15_16070"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020712087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEU4719362.1"
                     /translation="MTKDFLFRFLREHKLAVISSLSSKGKPESALVGIAVTENLELIF
                     DTVMSSRKYSNIIAHPGISAVIGWENETTVQYEGVASVVKSDEEIFREIYFDAFPDGR
                     ERFKTWPGLVHFVVRPKWIRYSSFHDPQQVEEMSW"
     gene            complement(3138..4535)
                     /locus_tag="VFU15_16075"
     CDS             complement(3138..4535)
                     /locus_tag="VFU15_16075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009580208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEU4719363.1"
                     /translation="MYIEQLYTNCLAEAAYWIESDGEAAVIDPLRETEPYLKKAQERG
                     VKIRYVFETHFHADFVSGHIDLAKATGATIVYGPGAETNYAVINARDNEEFQLGKITL
                     RVLHTPGHTPESSCFLLLDENKKPNAVFTGDTLFVGDVGRPDLLDGKMTKEELASMLF
                     DSLNNKLKPLPDDVIMYPAHGAGSACGKNIGKETFSTIGEQKKMNYALRISDRSEFIK
                     AVTDGLSAPPQYFFKDAMINKKGYESISQVMARNVKPLDAEKTALAIENGALVLDTRD
                     KDEFCKGFIPGSLFIGLDGTYAVWVGTLVDINTPLVVVAAEGKESEAILRLARVGYEN
                     VAGYLEGGIKSWIDAGNPVDRIESVAPETFAENVVSGKDHSTVLDVRREGEFANGHLK
                     GAAHLCLTKFSDEKNLASLDREKNYSVHCQSGYRSVIAASILKAKGFTHIVNIQGGWS
                     KLRTTKVPLELPELA"
     gene            4917..>5781
                     /locus_tag="VFU15_16080"
     CDS             4917..>5781
                     /locus_tag="VFU15_16080"
                     /inference="COORDINATES: protein motif:HMM:NF024502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase SAM-like domain-containing
                     protein"
                     /protein_id="HEU4719364.1"
                     /translation="MPVTLRKAKIKTGYSFYFDISYKGKRWTEWTKIHVNEKDSPKGK
                     KDTPEQKSEKIELAQKMKSEREREIVVLGKGLPGEIHLRERDFFDVYDELCAPRIRSA
                     HICVNIRKKIKKFNGENKPLPILSINKAWLANFLAFLKREGMVNNTVHQYFAFMGTIL
                     REAMCLGYIVQNPHSLFGRHERPKLKRPKADHLTAEELQRFVQKETKNVDEQLKQMFL
                     FSCFTGLRWSDCQRVTWKHIRNINVKGESQQVIVMNQQKTSDQVIVPLTPSAKALLDT
                     LRSEIMQSKGKG"
BASE COUNT         1439 a         1511 c         1514 g         1317 t
ORIGIN      
        1 acttcgctcc tgaaatgtgt accatcttcc cgcggatgtg ggagatgaac cagaaagtac
       61 acgtcaagga atacgaaagt tggggcaacg tggcgcacaa ccacgtgacc gtgcgtattc
      121 ccgaccgtca aacaggggaa atgcagtcgt acgaaaaacc gaccatgctg gccaacctga
      181 cgtacttctt ccgctaccag gtcaactgga tgtacatgcg ttatttcttc tggaatttcg
      241 tcgggcggca gaacgatatc cagggacatg gaaacctgac ggacggaaat taccagagcg
      301 gtattgcggc tttcgataaa gtcgacagca agaaggagcc gaaacgattg cgtgaaaatg
      361 ccgcgaacaa caagttctat tacatcccgc tgatcctcgg attgctgggc atgtacttcc
      421 atttcgcgaa ggactggaag agcgcgctgg tgatcgggct gctgttcctg ttcaccgggc
      481 ttgcgatcgt cgtgtacctc aaccagtatc cgcttcagcc gagggagcgg gattacgcct
      541 acgcgggatc gttctatgca ttcgccttct ggataggttt cggcgtgtat gcgctgtacg
      601 atctgttcac ggaaaggctg aagctgcagg gcaccatggc ggcgggactc gccacagtga
      661 tgtgtttttc gggcccgtac ctcatgggcc agcagggatg ggatgatcac aaccgatcca
      721 atcgttacac ctgccgcgat ttcgctgaaa actatctttc cacctgcgat aagaacgcca
      781 tcctgttcac caacggcgat aacgatacgt tcccgttatg gtacgcgcag gaagtggaag
      841 gttaccggac ggatgtgcgc gtgctcaacc tgagcctgct gaatacagac tggtacatcg
      901 accaggccaa ccggaaagcg tacgacagcg accgtgttcc gttctcgctt ccttcgacgc
      961 gttaccgcca gggttcctgt gaccaggttt tcttcgatcc cgataaaaag aacaagacga
     1021 aggaaatgag cgcacgcgag ctggtggaca tgatcattca cgccaaccct gatgcgatga
     1081 gcgtccgccg cggtgaagat gataacggcg atgtataccc ggatacggtt ttcgtgctgc
     1141 cgacaaataa aatttacatc cctgtcgata agaagctcgt gaaaagcaac ggaaccgttc
     1201 ctgccgacac gccggacagt ctcatcgccg accgcgtcga gtggacgatc aacaaatcgt
     1261 acctgctgaa agcagacctg atgatcctcg atcttcttgc ttcgaacaac tggcagcgcc
     1321 cgatctattt cgccgttaca gccggcagcg acagctacct caacctcgaa ggctggttcc
     1381 gcctcgaagg actggcgtac cgcctcgtgc cgatgaaggc agacaagcgc ggcattttct
     1441 ccgaaccggt tggctgcaac acggacatga tgtacgacaa gatcatgaac aagttcaagt
     1501 ggggcggact cgaagacaag tcgaagaaga tctacatgga cgagaacaac ctgcgcttca
     1561 ccacgaacca gcgcctccag atgatgacgc ttgcgaagct gctgaatgac gaaggcaagc
     1621 acgataaagc gaagaaagta ctcgatcgct gtctcgaagt gatgccggaa gagcatgttc
     1681 cgtacgaacc ggcaatggtg tacatggtgg atggttatta ccgcgccggc gccatggata
     1741 atgccaacaa gctttcgaaa acgctgttcg atcagtgcga agaagaatac aatttctaca
     1801 attcgcttgc aggcaaaaca ccgcagggtg gatacgatga tgacgccaac cgccttgcca
     1861 gcgcgctgca gatgctgagc cagttcgccg acaaatacgg gcagaaggac atgtccgcgg
     1921 attacaggaa acgactgacg gcgatgggac tcggcgcacc tacagaccag cccgatttcg
     1981 gtggcgatga cggtaacggc ggcatggatc cgaagctgaa ggcgcgcatc gattccatga
     2041 tgaatgcgat gaagcaggat tccggtaaga agaaacagca gccgccacat aaataggaat
     2101 gcgcatcctt gcacgtccgc cgtttttact ccggcagcta tacccgggcg cggcgtggag
     2161 gatgaatgcc tccgaaaaaa aaatatttct tacgttcgat gacggaccgg tgcccggggt
     2221 aactcccgcg gcactttccg ttttaaagga gcaggacgtc agggccacgt tcttctgcgt
     2281 gggtgaaaac gtgcagcgtc accccgaaat tttttcttcg atccttgaac agggacaccg
     2341 tgtaggcaat catacgttcc atcatgcgga cggatggaag cacacttccc gctcgtacct
     2401 gcgcgaggtg cgccagtgcg cggaggtggt gaaatcggat cttttccgtc cgccctacgg
     2461 gcgcatgcgc cgctcgcagt tcgaagccat caggcggaaa tataaagtga tcatgtggga
     2521 cgtgcttacc tgcgactttg acctgtcgct ttcctgcgag gatgtgctca gcatcgcggc
     2581 cggacattcg cggccgggtt cggtgatcgt ttttcacgac agcctgaaag cgaaggaacg
     2641 catgctttac gttctgccga aatacattgc tgaaatgaaa acgcgcggat tcgctttcga
     2701 cgtgctctga cctaccagct catttcttct acctgctgcg gatcatggaa gctgctgtac
     2761 cggatccatt tcgggcggac gacaaaatgc accaacccgg gccaggtttt gaatcgctcc
     2821 cttccgtccg gaaatgcgtc gaaataaatt tcgcggaata tttcttcatc gcttttcacg
     2881 accgaagcga caccttcata ctgcacggtc gtttcgttct cccaaccgat caccgccgag
     2941 atcccggggt gcgcgataat attggaatac ttgcgtgagc tcatcacggt atcgaagatc
     3001 agctcgaggt tttccgttac ggcaatgcca accagcgcgg attcgggttt tccctttgac
     3061 gaaagggagg aaatgacagc gagtttatgt tcgcgcagga aacggaaaag aaaatccttc
     3121 gtcattttta cggcgcatca cgccagctcc ggcagctcca gcggaacttt tgttgtacgg
     3181 agtttgctcc atccgccctg tatgttcacg atgtgcgtga atcctttcgc cttgagaatg
     3241 gatgctgcga taacggaacg gtaaccgctc tggcaatgca cggaataatt cttttcacgg
     3301 tcgagtgaag ccaggttttt ttcgtcgctg aatttcgtga ggcagagatg cgcagctcct
     3361 ttcaggtggc cgttcgcgaa ctcaccttcg cggcgcacgt caagaacagt gctgtgatct
     3421 tttccgctga cgacgttttc tgcaaatgtt tccggcgcaa cggattcgat cctgtcaacc
     3481 gggttgccgg cgtcgatcca tgattttatt ccgccttcga ggtagccggc cacgttctcg
     3541 tagcctacgc gggcaagacg caggatcgct tcgctttctt ttccttcagc tgcgacaacg
     3601 accaggggcg tattgatatc caccagcgtt cccacccaca ccgcgtacgt tccgtcgagg
     3661 ccgatgaaaa gcgatccggg aataaatcct ttgcagaatt catccttgtc gcgcgtatcg
     3721 agcacaagcg ctccgttctc gatggccagc gctgtttttt ccgcgtcgag cggcttcacg
     3781 ttgcgcgcca tcacctgcga aatgctttcg tatcccttct tgttgatcat cgcgtccttg
     3841 aagaaatact gcggaggcgc ggaaagtccg tcggtaaccg ctttgatgaa ttcactgcgg
     3901 tcgctgatgc gcagggcgta attcattttc ttctgctcac cgatggtgga aaaagtttcc
     3961 ttgccgatat tttttccgca ggccgacccg gctccgtgcg cgggatacat gatcacgtca
     4021 tcggggagcg gcttcagctt gttgttcagt gaatcgaaca gcatggaagc gagctcttcc
     4081 ttcgtcattt ttccgtcgag gagatccggg cgccccacat cacctacaaa cagcgtgtcg
     4141 ccggtgaaaa cggcattcgg ttttttgttt tcatcgagga gcaggaaaca gctcgattca
     4201 ggcgtatgcc cgggcgtatg gagcacgcgc agcgtgatct ttcccaattg aaactcttcg
     4261 ttgtcccgtg cgttgatcac ggcatagttc gtttcagcac cgggaccgta aacgatcgtt
     4321 gcgcccgttg cttttgccag gtcgatgtga ccggaaacaa aatccgcgtg gaaatgcgtt
     4381 tcaaaaacat agcggatctt cacgccgcgt tcctgcgctt ttttcaggta gggttctgtt
     4441 tcgcggagcg gatcgatcac cgcggcttca ccatcggatt cgatccagta agcggcttcc
     4501 gccaggcaat tcgtatagag ttgttcgata tacatgttcg ggcttttttg acactgcaaa
     4561 gatacgaagc tcgtgttgat aactcccccc ggaaaccaaa agcggttaaa agccaatact
     4621 ggcgcgggtt tcagccattt tcccggcttt tggtaggttt ttcttagctc ctgtttctcc
     4681 ttaacttggt taggaaaccg ttaggaaaac agccctaaaa agcccctaaa aatgagccat
     4741 tttaagccga ttagaccgcc gccccgcgca cccccgtttt aaccgcaaaa accgtcttta
     4801 ggacggtttt acggtttctg cgaggtttcg tacaccagcg cggaaaaatg cggcctaatg
     4861 catagcatag gcgcacaact gtaaaccaaa aatagaacta tttaactcta aatacaatgc
     4921 cagtaacatt aagaaaagca aagattaaaa cgggatactc gttttacttc gatataagct
     4981 acaaagggaa acgttggacg gaatggacta agatacacgt caacgaaaaa gacagcccca
     5041 aagggaaaaa ggacacaccc gaacagaaaa gcgaaaagat cgaattagcg cagaaaatga
     5101 aatcggagcg cgaacgcgaa atagtcgttt tgggaaaagg tttgcccggt gaaatccatt
     5161 tacgggagcg ggattttttc gacgtgtacg acgaactttg cgccccgcgc attcgttccg
     5221 ctcacatttg cgtcaatatc cgtaagaaga ttaaaaaatt caacggagaa aataaacccc
     5281 tgcctatcct ttcgattaat aaagcatggt tagctaactt ccttgctttc ctgaaacggg
     5341 aaggaatggt aaataacact gttcaccaat actttgcttt catgggtact attctgcggg
     5401 aagcaatgtg tttagggtat atcgtgcaaa acccgcatag tcttttcggg cggcacgaac
     5461 gccccaagtt gaaacgccct aaagcggatc accttacagc ggaagaatta cagcggtttg
     5521 ttcaaaagga aacaaagaac gttgacgaac agttaaagca aatgttcctc tttagctgtt
     5581 tcacggggct acgctggagc gactgccagc gggtaacgtg gaaacacata cgaaacatta
     5641 acgttaaggg cgaatcgcaa caggtaatag tgatgaacca acagaaaacg agcgatcagg
     5701 taatagttcc gcttacccct tcggcaaaag ccctgttaga taccctgcga agcgaaataa
     5761 tgcaaagcaa aggcaaagga t
//
LOCUS       DASTSF010000568         4070 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1225112,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000568 DASTSF010000000
VERSION     DASTSF010000568.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4070
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1225112"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="VFU15_16085"
     CDS             <1..297
                     /locus_tag="VFU15_16085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719365.1"
                     /translation="MVAFFASCGNDEAEQKRKADSAHMADSMAQIEAARKADSAHMAD
                     STKNAMDAAEAKRKADSAHNADSIANAKPGKGKTPKATPPKSNTPPPPKVGGHR"
     gene            450..725
                     /locus_tag="VFU15_16090"
     CDS             450..725
                     /locus_tag="VFU15_16090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719366.1"
                     /translation="MKKFLALAAVAGMAVLASCGNAEEAAKKMQDSINAADSMARVQH
                     MADSTKMADSTKKAADDMMAKHVADSTAAAAAHQDSIDKKLIKMPKK"
     gene            991..2700
                     /locus_tag="VFU15_16095"
     CDS             991..2700
                     /locus_tag="VFU15_16095"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEU4719367.1"
                     /translation="MEPVVNTKHNLIFIVALLMIFTAGLTVDVMNVDAAQYASMSRDM
                     LFSGRWLEVQCRHHDYLDKPPLLFWLSALSFKIFSVSNWAYKLPSFLFAILGIYSTYR
                     LAELLYDRRTGSVAALMLASCFGVFIMTNDIRTDTLLLGSVVFSIWQLLLYLKTKRWP
                     SLVAGFAGIAVAMLAKGPIGLVIPVMALSCEFAYRRQWKNFFRPEWIAGAIIIAVILF
                     PMSLGLYRQFDLHPEKTVNGSTGVSGLKFFYWTQSFGRITGDSDWGTKYDNGAGPFFF
                     THTFLWAFFPWSFLVLGGLIKTFIKLVRSRFREGFVPEVISAGGFTLTFIALSLSKYK
                     LPHYIYVTFPLAAILAARFFLYDVFHHMKKYLLVIFTSLHVLFIIALAAASLLITFFI
                     FPGGHTIDKITVVFWLTAAIVMLLFLKNTYRRILYPLVAALAGFYFTGNAIFYPSLLR
                     YESSALAGKLVVKNNVPPDDLFTYHDIFEYALDFYSGRDVEYTSLEDSAFTNAMSRHG
                     SVWIYTEASALDEISAKFSIDQKQEFSDYPVQFLKAEFLDPSRRESTLRKRYLLVISP
                     KKE"
     gene            complement(2701..>4070)
                     /locus_tag="VFU15_16100"
     CDS             complement(2701..>4070)
                     /locus_tag="VFU15_16100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEU4719368.1"
                     /translation="WSNIVTVMLLLLFNWVHKFDVFQNSTRVLGMNYSVAPAKYTNEE
                     LRDLGLNPQWRKEDSLNTIAILENWKAKNTTDPQQKPKLVILSCSGGGLRSTLWTFYT
                     MQYLDSLTGGQMMKHMVLICGSSGGMVGAAYMRELCLREEEGEKINHLDPAYRENAGE
                     DVLNPMAFSIAVNDWFLPLRHAEYDNMDYSRNRAWAFEEKMLSNTGNVLEKKLSDYTK
                     PEREAKIPMMFFAPTIVNDGRKLIIASQGVSYLSQPAPPAGVSNQALPDGVEFSRFFS
                     NQGAANARFMSVLRMNATFPYITPLTDLPSEPVIEVFDAGMRDNFGVDNLLRFLYTFR
                     TWISENTSGVVLLQTRDKSKIRPVEETHSQTVMQALSRPMGSFYGNLFVVQDYNHDYA
                     IDQAGHWLGAPIDVLDFELTNDEPDLISLSWHLTGHEKTRILSCMSAPGNQKAAARFR
                     YLLTQ"
BASE COUNT          919 a         1104 c         1053 g          994 t
ORIGIN      
        1 atggtagcgt tcttcgcttc ctgcggcaat gacgaagcag aacagaagcg taaagctgat
       61 tctgcacaca tggccgattc aatggcacag atcgaagcag cccgcaaggc tgactccgca
      121 cacatggctg attctacaaa gaacgctatg gatgctgcag aagctaagcg taaagctgac
      181 tctgctcaca acgcagattc tatcgccaat gcaaaacccg gaaaaggcaa gactccgaag
      241 gctactccgc cgaagagcaa cacgcctcct ccgccgaaag ttggcggaca ccgctaatcg
      301 gttggttcaa gcgagaaaca aaaagggaag cacagcttcc cttttttatt gttgatttcg
      361 gtgttaaaaa aaagttcaaa agtttgcggc tcaaaaaaat cttactatat ctttgcgcca
      421 gttttaacca aaccaaccaa ataaccaaaa tgaaaaagtt tctcgctctc gctgctgtag
      481 ccggtatggc agtacttgct tcctgcggca acgcagaaga ggctgcaaaa aaaatgcagg
      541 attccattaa cgctgctgat tcaatggcgc gcgttcagca catggctgac tcaaccaaaa
      601 tggctgattc aaccaaaaaa gctgccgatg acatgatggc aaaacacgta gctgattcta
      661 ctgcagctgc tgccgcacat caggattcta tcgacaagaa gctgatcaaa atgccgaaga
      721 agtaattctt cagctgagaa aagacgggga gcggtttgcg ctcccttttt ttttgtcagt
      781 cgcacttgca atccggacca cagttcttgt cacgtgcagc ttttttgaca cgcttcagga
      841 aacgcagcgc gagaaagccg gcggaacccg tgaacagcaa caacagtatg acctggtcgg
      901 cggacatggc agaaagcggt aattttccgc ttccgtaaag ttcgtcattc cttttaaacg
      961 tttccgggaa acacaccaaa ttcttccctc ttggaacccg tcgtcaacac gaagcacaat
     1021 cttattttta tcgttgccct gctgatgatc ttcacagcag gactcacggt ggatgtgatg
     1081 aacgtcgatg ccgcccagta cgcttccatg tcgcgggata tgctgttttc cgggcgatgg
     1141 ctggaagtgc aatgccgcca ccacgattac ctcgacaagc cgccgctgct cttctggctc
     1201 tcggcgctca gctttaagat cttcagcgtc tccaactggg cctacaagct cccttcgttc
     1261 ctcttcgcga tcctcgggat ctattccacc taccgcctcg cggaattgct gtatgacagg
     1321 cgcacaggtt cggttgcggc gctcatgctc gcctcgtgct tcggcgtttt catcatgacg
     1381 aatgacatcc ggacggacac attgctcctc ggcagcgtgg ttttctcgat ctggcaattg
     1441 ctgctttacc tgaaaaccaa gcgctggccc agcctggttg ccgggtttgc gggaattgcc
     1501 gtggcgatgc tcgccaaagg cccgatcggc ctggtcatcc cggtgatggc cctgagctgc
     1561 gaattcgctt accggcggca gtggaagaat ttcttccgcc cggagtggat agcaggcgcg
     1621 attataatcg cggttatctt atttcccatg tcgctgggac tttatcgcca gttcgacctt
     1681 cacccggaga agacggtcaa cgggagcacg ggtgtttcgg gactgaaatt tttctactgg
     1741 acccaaagct tcggccgcat cacgggagac agcgactggg gaaccaagta cgataacggg
     1801 gcaggtccgt tcttcttcac gcatacgttt ctctgggcgt tttttccctg gagcttcctg
     1861 gtgctgggcg ggctcatcaa aacgttcatc aagcttgtgc gcagccgttt ccgggaaggt
     1921 tttgtgccgg aagttatttc cgcaggcgga tttacgctga cattcatcgc cctttcgctt
     1981 tcgaaataca agctgcccca ttacatctac gttacgttcc cgctggccgc cattcttgcc
     2041 gcacgctttt tcctgtatga tgtttttcat cacatgaaaa aatacctgct cgtgatcttc
     2101 acttcgcttc acgtgctgtt cattatcgcc ctggccgccg catcactgct catcacgttc
     2161 tttatttttc ccggcggcca cacgatcgac aagatcacgg tcgttttctg gctgacggcc
     2221 gccatcgtca tgctgctgtt cctgaagaac acttaccgcc ggattcttta cccgctggtg
     2281 gcggcgctgg ccggttttta ctttacggga aacgccattt tttatccttc gctcctccgg
     2341 tatgaatctt cggcgctggc ggggaagctg gtggtgaaga acaatgttcc gccggacgac
     2401 cttttcacct atcatgacat tttcgaatat gcgctcgatt tttacagcgg aagggacgtg
     2461 gaatatactt cgctggaaga ttccgctttt acgaacgcaa tgagcaggca cggaagcgtg
     2521 tggatctata cggaagcatc cgcgctcgac gagatttccg caaaattctc catcgatcaa
     2581 aagcaggaat tcagcgacta cccggtgcag ttcctgaaag cagaattcct cgatccttcc
     2641 aggcgcgaaa gcacgctgcg aaagaggtat ctcctggtta tttccccgaa aaaagaatga
     2701 tcactgcgtg agcaggtaac ggaaacgcgc ggcagctttt tgattcccgg gcgccgacat
     2761 gcacgaaagg attctcgttt tttcgtggcc ggtcaggtgc cagctgagcg agatcaggtc
     2821 gggttcgtca ttcgtcagct cgaaatccag cacgtcgatc ggcgcaccca gccagtgccc
     2881 ggcctggtcg atggcgtaat cgtggttgta atcctgcacc acgaaaagat tcccgtaaaa
     2941 gctgcccatg ggtcgtgaaa gcgcctgcat gacggtctgc gaatgcgttt cttccacggg
     3001 gcgtatttta cttttatccc gcgtctgcag cagaacaacg ccggatgtat tttcagaaat
     3061 ccatgtgcgg aacgtgtaga gaaaacgcag caggttgtcg acaccgaaat tatcgcgcat
     3121 tcccgcgtcg aaaacttcta tgacgggttc gctgggaaga tccgtaagcg gggtaatgta
     3181 cgggaacgtg gcgttcatgc gcagcacgct catgaaacgc gcgttggcag cgccctggtt
     3241 gctgaagaag cggctgaact cgacgccgtc ggggagcgcc tggttcgaaa cgccggccgg
     3301 gggcgcgggc tgcgacaggt aagaaacgcc ctgcgaagcg atgatcagct tccgcccgtc
     3361 gttcacgatg gtgggagcga agaacatcat cgggatcttc gcttcacgtt ccggtttcgt
     3421 gtagtcggaa agctttttct cgagcacatt tcccgtattg gaaagcattt tttcctcgaa
     3481 cgcccacgca cggttgcggc tgtaatccat attgtcgtat tccgcgtggc gaagcggtaa
     3541 aaaccagtcg ttgacggcaa tggaaaaagc catgggattg agcacatctt cccccgcatt
     3601 ttcccggtaa gcgggatcaa gatgatttat tttttcccct tcctcttcgc gcaggcaaag
     3661 ctcgcgcatg tatgctgctc ccaccattcc gcccgaagag ccgcagatga gcaccatgtg
     3721 tttcatcatc tgcccgccgg tgagactgtc gaggtactgc atcgtataaa atgtccacag
     3781 cgtggaacgc agtccgccgc cgctgcagct gagaataacg agcttgggtt tctgctgggg
     3841 atccgtggtg tttttggctt tccagttttc gagaatggcg atcgtattga gcgaatcttc
     3901 ttttctccat tggggattca gaccgagatc ccgcagctct tcgttggtgt atttcgccgg
     3961 tgcaaccgaa taattcattc cgagaacgcg cgtcgaattc tggaacacat cgaatttgtg
     4021 cacccagttg aatagcagca gcagcatgac ggtaacgata ttcgaccagc
//
LOCUS       DASTSF010000569         4817 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1896116,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000569 DASTSF010000000
VERSION     DASTSF010000569.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4817
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1896116"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..482
                     /locus_tag="VFU15_16105"
     CDS             <1..482
                     /locus_tag="VFU15_16105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HEU4719369.1"
                     /translation="LINSSLDGDRIIVKKDINIGMATALPSGNLIVPVIRNADQLNLV
                     GLAKQVNTLADNARNNKLRPEDIADGTFTLTNVGTFGSLMGTPIINQPQVAILAVGAI
                     RKKPAVIETPSGDTIGIRHMMYLSHSYDHRVVDGALGGMFVRRVADYLEQWDMNREI"
     gene            697..1146
                     /locus_tag="VFU15_16110"
     CDS             697..1146
                     /locus_tag="VFU15_16110"
                     /inference="COORDINATES: protein motif:HMM:NF044334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719370.1"
                     /translation="MKHVIFYLLLLVSVSACDKKGKDTIYLVPEGFHGRAVIFFDVEN
                     GDPEAAENGSVVLRLKENGCLLTRSQPNFGIFKQTFYFCDSLGKRTCIPEDVEFTSDT
                     LPSIYGEVTSSVDHNGKKVCIFSFLVCTKKEYPELNRTDIDTSCLDK"
     gene            1322..3370
                     /locus_tag="VFU15_16115"
     CDS             1322..3370
                     /locus_tag="VFU15_16115"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEU4719371.1"
                     /translation="MKKTLFFAASLLAVIGIFIAAGIPRNVRQKISQGDNYFAQDDYT
                     DALPLYLDAYKLDPTNANLCFKIGVCCLNIPSRKHDAENYLFVAASNVSADYRETSMK
                     ERHAPPSAYYYFGQALHLNNKFDNAIDAFSKFKAYIDTTDTKTINDVNLRIRWCENGK
                     TLVNSPVNIKVENIGPNVNTKYPEYSPVISADERTLIFTSRRPTNTGGLIDQRDGMYF
                     EDIYVSTRASDSSAWGPAVQIGNNINTPGHEATIGISADGQTLYIYKDDNGDGNIYSS
                     LLNGQSWSSPVKLTTNVNSKSWEPSACVTPDGNTLYFSSTREGGYGGRDIWRSVKLPN
                     GEWSLPTNLGPKINSAYDEDAPSILADGKTLYFSSNGTQSMGGFDIFSTEFSEDSGWA
                     APVNIGYPVNTADDDIFFVPTPDNKHAYYSSASSPGGQGEKDICYLTFPDKEETHLTV
                     LEGEITSIFGGVPDGTVITVTDVETGELVGQYTPNSATGRYVIVLPSGRNYSITYEAT
                     DYLFQSDNMEVSDTTAFQTIERPVELAPLKVGQKIIVRNIFFDSGKSQLKPESKAELD
                     KLVTLMTQFPKLIVEISGHTDASGPDDLNQKLSEQRAQSVADYLVAHGIDRSRLRTVG
                     YGESRPIAINYNKNGTPNRQGMALNRRFEFTILSVDGQIKDVVEPIAVPDSLKNAPKK
                     "
     gene            3623..>4817
                     /locus_tag="VFU15_16120"
     CDS             3623..>4817
                     /locus_tag="VFU15_16120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719372.1"
                     /translation="MQGNLLIEEENWPMALSYFQEAYKVDSTNANINYKIGICYLHSA
                     SEKANSLKYLRVAAKGISRNYDPYDAKSKKAPENTYYYLAQSYHYNYQFDSAISFFET
                     YKAILGNRDPETTKDIDTRIGWANNAKEFMASPLPVTITNLGDSINTQYPDYSPVISI
                     DENTIIFTSRRSGDKGIDGQYYEDILIADKKDDGTWGAAHPISPYINTPTNEASISLS
                     ADGQTLFIYKDDNGGDIYQSTQMNGQWMTPVPMGGDINSKYWETHACLSADGNTLYFV
                     SDRPGGFGGRDIYRVVKLPNGKWSKALNLGPTVNTPEDEDAPFIHPDQKTLFFSSKGH
                     KSMGGFDIFFTSLNDSGKWAEPINVGYPINTPDDDIFYVTSPDGKRAYFSSVRQGGYG
                     EKDIYE"
BASE COUNT         1233 a         1339 c         1185 g         1060 t
ORIGIN      
        1 cgctgatcaa cagctcactc gacggcgacc gcatcatcgt aaagaaagac atcaacatcg
       61 gcatggccac agcattgccc agtggcaatt taattgtgcc cgtgatccgc aacgccgatc
      121 agctaaacct ggtgggcctg gccaaacagg taaatacact ggctgacaat gcccgcaaca
      181 ataagttaag gcccgaagat attgccgacg gcaccttcac ccttactaac gtgggaacct
      241 tcggaagcct gatgggcacc cctatcatca accagccgca ggtggccata ctggccgtgg
      301 gcgcgatccg caaaaaaccc gcggtgatcg aaacgccctc cggcgatacg atcggcatac
      361 gccacatgat gtacctctct cattcctacg accaccgcgt ggtggacgga gcgctgggcg
      421 gaatgttcgt ccgccgcgta gcggattacc tcgagcagtg ggacatgaac cgcgagatct
      481 gaaacaggaa aaccgcaaat ttccggtaag gtcaaattca ttggcgcatt ttgtgtttcc
      541 ttgcggtgct tgaaatcctg tacctgaagg ttagctcagg acatttacac acagaaggtc
      601 gataaggatg acgggccagg actgatcaac ctgaacagtt cttccgtatt caagagctcg
      661 atcacttaca ttgacctctg atccaaagcc tgttccatga agcacgtaat cttctatctc
      721 ctgctgcttg tgtcggtatc tgcgtgcgat aaaaaaggga aagacacgat ctacctggtc
      781 ccggaagggt tccatgggcg cgccgtcatt ttctttgacg ttgagaatgg tgatccggaa
      841 gcagcggaaa acggaagcgt tgttctccgc ctgaaagaaa acggttgcct cctcacacgt
      901 tcacagccga acttcggcat tttcaaacag accttttatt tctgtgactc gctcggaaag
      961 cgtacctgca ttcccgaaga tgtggagttt acttcagata cgcttccctc catttacggc
     1021 gaggtgacca gctccgtgga tcacaatgga aaaaaggtct gcattttttc ttttctcgtc
     1081 tgcacaaaga aagaataccc cgaattgaac aggacggaca tcgatacaag ctgtcttgac
     1141 aaatagttcc tgccacgatt aaggaagact aacccgggct taccccttca tcccgcaacc
     1201 ggccctgatt cggcaggaca ggttggtttg cttgcaaagc gtgacgtatc cgcacggtcc
     1261 tgcttcaatt ttgatgaatt ttcccggcgg gtgcctcgtg ttttttatct ttggttcgtc
     1321 catgaaaaaa acactctttt tcgctgcgtc tcttcttgcg gtcattggca tttttattgc
     1381 cgccgggata cccaggaacg tgcgccagaa aatatcgcag ggcgataatt atttcgcgca
     1441 ggatgattac accgacgcgc ttccgctcta tctcgatgcc tacaagctcg atcccacgaa
     1501 tgccaacctc tgcttcaaga tcggggtctg ctgcctgaac attccttcgc gcaagcacga
     1561 tgcagaaaat tatcttttcg tcgccgcttc gaatgtttcc gcggattacc gggaaaccag
     1621 catgaaggag cgccacgcac cgcccagcgc gtattactac ttcgggcagg cgcttcacct
     1681 caataacaag ttcgacaacg cgatcgatgc gttcagcaag ttcaaagcat acatcgacac
     1741 gaccgacacg aaaacgatca atgatgtaaa cctccgcatc cggtggtgcg aaaacggcaa
     1801 aacgctggtc aattcacccg tcaatatcaa agtggaaaat atcgggccga acgtcaatac
     1861 gaagtatccc gaatattctc ccgtgatatc ggccgatgaa cgcacgctca tcttcacctc
     1921 ccgccgtccc accaatacag gcggactgat cgaccagcgc gacggcatgt acttcgaaga
     1981 tatttacgtc agcacgcggg cgagcgattc atccgcgtgg ggaccggccg tgcagatcgg
     2041 caacaacatc aatacgcccg gccacgaagc cacgattggc atttccgctg acgggcagac
     2101 gctctacatt tacaaagacg ataacggcga cggaaacatt tactccagcc tgctcaacgg
     2161 gcagagctgg tcgtcgcccg taaagctgac caccaatgtc aacagcaaat cgtgggaacc
     2221 cagcgcctgc gttacgccgg acgggaacac actttatttt tcgagcacgc gcgaaggagg
     2281 ctacggcggg agggacatct ggcgttcggt gaagctgccc aatggcgaat ggtcgctgcc
     2341 caccaatctc ggtccgaaaa taaattccgc gtacgatgaa gatgcgccat ccattctcgc
     2401 cgatggaaaa accctttatt tttcatcgaa cggaacacag tcgatgggcg gcttcgatat
     2461 tttttcgact gaattttcag aagacagcgg ttgggccgcg ccggtgaata tcggctatcc
     2521 cgtgaatacc gccgacgacg atattttctt cgttcccacg ccggataata agcacgctta
     2581 ctattcttcc gccagcagtc ccggcgggca gggtgaaaag gacatctgct acctgacgtt
     2641 ccccgataag gaagaaacgc accttaccgt cctcgagggc gagatcacca gcatcttcgg
     2701 cggcgttccc gacggaaccg tcattaccgt caccgatgtg gaaaccggcg agctggtcgg
     2761 gcaatacacg ccgaacagcg caaccgggcg ttacgtcatc gtccttccgt ccggaagaaa
     2821 ttacagcatc acctacgaag cgacggatta tcttttccag tcggataaca tggaagtttc
     2881 cgacacaacg gcgttccaga ccatcgagcg cccggtggag ctcgccccgc tgaaagtcgg
     2941 gcagaagatc atcgtccgga atattttctt tgattcgggg aaatcacagc tgaagcctga
     3001 atcgaaagcc gagctcgaca aactggtgac gctgatgacg cagttcccca agctgattgt
     3061 cgaaatatcc gggcataccg atgcaagcgg accggatgat cttaaccaga aactttccga
     3121 gcagcgcgca caatccgttg ccgattatct tgtggcgcat ggcatcgacc ggtcgaggct
     3181 gcgcacggtc ggttacgggg aatcacgtcc tatcgcgatc aactacaaca agaacggaac
     3241 accgaacagg cagggaatgg ccctcaaccg ccgctttgaa ttcaccatcc tcagtgtcga
     3301 tgggcagata aaagacgtgg tggaaccgat cgctgtaccc gacagcctga agaacgctcc
     3361 gaaaaaataa tgcgccggaa cggtttgcat gcattctcca catacccgcc gactgatttc
     3421 acatgatgat gttagttgat ttaaggtcat ttggtctcta ataattcagg gaaaggttta
     3481 tttttgcatg atgatcgccg taacaggtgg ctttcaggca tttatgaaat taaccaggct
     3541 tttcgttttc tgtttcctct tattgttgcc cttaatttcc cgtgcccagg acccgatccc
     3601 cggagattat acgcaaaatg tcgtgcaggg aaacctgctg atcgaggaag agaactggcc
     3661 gatggcgctc tcctatttcc aggaagctta caaggttgat tcgaccaatg ccaacatcaa
     3721 ttacaagatc ggcatctgct atctccactc cgcttccgaa aaagccaact cgctgaagta
     3781 cctgcgcgtt gcggccaaag gaatttcacg gaactacgat ccgtacgatg ccaaatcgaa
     3841 aaaagcgccg gaaaacacgt actactatct cgcccagtct tatcattaca attaccagtt
     3901 cgattccgcc atcagctttt ttgaaacgta caaagccatt ctcggcaacc gtgatcctga
     3961 aacgacaaaa gacatcgaca cacggatagg ttgggcgaat aatgcaaaag agttcatggc
     4021 ttcaccgctt ccggttacca ttaccaacct cggcgacagc atcaacacgc aatatcccga
     4081 ttacagcccg gtgatctcca ttgacgagaa cacgatcatt tttacgtcaa gacgttccgg
     4141 cgataaagga attgacgggc agtattacga agacattctc atcgccgaca aaaaagacga
     4201 cggtacatgg ggcgcggcgc acccgatatc gccttacatc aatacgccta cgaatgaagc
     4261 ttccatctcg ctttccgctg acgggcaaac gttgttcatt tacaaagacg ataacggcgg
     4321 agatatctac cagagcacac agatgaacgg gcagtggatg acgccggtac cgatgggcgg
     4381 cgacatcaac agcaaatact gggaaacgca cgcctgtctt tcggccgacg ggaacacgct
     4441 ttatttcgtg agcgatcgtc ccggcggatt cggcggccgc gacatctacc gcgtcgtcaa
     4501 gcttccgaat ggaaaatggt cgaaggccct gaatctcggt ccgacggtga acacgcccga
     4561 agacgaagac gctccgttca ttcaccccga ccagaaaacg cttttctttt cgtccaaagg
     4621 acacaaatcg atgggcgggt tcgatatttt cttcacgtcg ctgaacgatt ccggcaaatg
     4681 ggcggaaccg atcaacgtgg gttacccgat caacacaccg gacgacgata ttttttacgt
     4741 gacctcgccc gatggtaagc gcgcttactt ttcttccgtg cgccagggcg gttacggaga
     4801 aaaggatatt tacgagg
//
LOCUS       DASTSF010000570         4951 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1227711,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000570 DASTSF010000000
VERSION     DASTSF010000570.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4951
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1227711"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..776)
                     /locus_tag="VFU15_16125"
     CDS             complement(<1..776)
                     /locus_tag="VFU15_16125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008236703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEU4719373.1"
                     /translation="MKRLFLFLLVLSCGFVKAQSLVSGIVTDAASGEPLVNAVVVVND
                     GKIFTLTDFNGAYHFSLADGTYTLSVKYVGYTCVPVTVKVSGKSVSLDFQCSSGNLKE
                     VEVIADVAIDRKTPVAFTTLSPKQIHEELNGRDLTMMLNSTPGAYASEQGGGAGDSRV
                     NIRGVDQRNVGVLVDGVPVNDMENGQVYWSNWDGLSDITRTMQVQRGLGASKLAIPSV
                     GGTINILTNGIDEKRGYSVSSTFGSDNLQKFSFGFNSGEFA"
     gene            complement(839..1741)
                     /locus_tag="VFU15_16130"
     CDS             complement(839..1741)
                     /locus_tag="VFU15_16130"
                     /inference="COORDINATES: protein motif:HMM:NF013125.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HEU4719374.1"
                     /translation="MKKQLLTILSLGFIVFANAQCSELFFSEIDEGSGNNKTFEIYNP
                     TNAPIALSNYRIIRFSNGSTVPTDSLTLSGTIAPHDVWVVANGQTTSTPTSPACDTAL
                     QNMADQLGGAYPDPCYQNGNDAIVLAKISPRTFVDIYGKIGEDPGQSWTDVFPYTDAA
                     GAYWTKDHSLIRKSSVTQGVTVNPTAFNPTTEWDSLPKNNWSNLGMHNCSCPTGISEI
                     KKSMVTLTVFPNPNDGHFTIDAGTKMESCEVYNTLGALVSSRVFTNGETKADLDLTGM
                     PDGLYLVQVRLADGHRMTARVSVR"
     gene            2025..2999
                     /locus_tag="VFU15_16135"
     CDS             2025..2999
                     /locus_tag="VFU15_16135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008612 - peptidyl-lysine modification to
                     peptidyl-hypusine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase family protein"
                     /protein_id="HEU4719375.1"
                     /translation="MNKGPISQFIDHHYRHFNAAALKDAAKGYETHLLEGGKMLVSLA
                     GAMSTAELGISLAEMIRQDKVHIISCTGANLEEDLMNLVAHSHYKRVPNYRDLTPQQE
                     WDLLEQGLNRVTDTCIPEEEAFRRLQKHIYELWKKADTNGERYFPHEFMYQMINSGVL
                     KQYYEIDPKNSWMLAAAEKNLPIVVGGWEDSTMGNIFASYVIKKEVKATTMKSGIEYM
                     VWLTEWYRANSGGKGVGFFQIGGGIAGDFPICVVPMMYQDLEWHDVPFWAYFCQVSDS
                     TTSYGSYSGAVPNEKITWGKLGIDTPKFIVESDATIVAPLIFAWILGW"
     gene            3109..>4951
                     /locus_tag="VFU15_16140"
     CDS             3109..>4951
                     /locus_tag="VFU15_16140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719376.1"
                     /translation="MAKAKAASKNIPVQTSFWQNDRNTGFALAAFAFLLYAQTISYGF
                     VLDDVAVVHGNEFVKSGFSGFGKILTTFYWHGFPNFATANSGLFRPVSLLLFATEWQL
                     FPGKPHVFHFVQVALYAICAYQLYRLLRELLRDHSGSLALMTTLLWIVLPVHTEVVAN
                     IKSADEVLSLLFFLLSFRKILAWSETGKTATLVSSAGLIFLSLLSKEGAVMFVPVMLI
                     ALMQFRNKTAKQLVLPAGVFLATGIVWLVWHSWVISHAGSAMITYDYRNNALLSSTSV
                     ADRTGTAIGMQARYWMKMLAGFPLSYNYSFNEIPVDGFAGVWTWIALAGIFAAGFFAY
                     KNFRKNPVVSFSLLFYFITFALTCNIFYLIGETFAERLAFVPSIGFCLLFSFLVLRFT
                     NGLREKRIHAQAMYVLVPLALVYSIRTFSRSQVWTDEPTLYTNDVDNAPNSARVHDNS
                     GVLLYNAALGTQDPNQKKQLFGQAYSEFSTAYTIDSVDFQAAHILAEIEFNRGNNEEA
                     IRWGRKNIACFRFFHVPPNDPTVYSLIGNAFMNLQQYDSAHAVFATAASQYPGNESFT
                     IALGGNSLAQKDTTGAISFFEKATSINPKSVAAWDKLANVRGMHRDYR"
BASE COUNT         1130 a         1311 c         1324 g         1186 t
ORIGIN      
        1 gcaaactcac cgctgttgaa cccgaaagaa aatttctgga ggttatccga tccgaaagtg
       61 gatgaaacgg aatagccgcg tttttcatcg atgccgttgg tgagaatgtt gatcgttccg
      121 cccaccgacg gaatggcaag cttcgaagca ccgagtccgc gctgcacctg catggtccgc
      181 gtgatgtccg aaagtccgtc ccagttgctc cagtacacct ggccgttctc catgtcgttg
      241 accgggacac catccacgag cacgcccacg ttacgctggt ccactccgcg gatgttgaca
      301 cgtgaatcgc ctgctccccc gccctgctcg gaagcgtacg cgcccggcgt ggagttgagc
      361 atcatcgtca ggtcgcggcc gttgagctct tcgtggatct gtttcgggga aagcgtggtg
      421 aacgctaccg gtgttttcct gtcgatcgcg acatcggcga tgacctccac ctccttcagg
      481 ttcccgctgc tgcattggaa atcgagtgaa acggactttc ccgaaacctt aaccgtaaca
      541 ggaacgcatg tgtagccgac gtattttacg ctgagcgtgt acgttccgtc ggcgagtgaa
      601 aaatgatatg cgccgttgaa atcggtcagt gtgaaaatct tcccgtcgtt caccaccaca
      661 acggcgttta ccagcggctc accgctggct gcgtccgtta cgattccgga gacgaggctt
      721 tgcgctttca caaaaccgca ggataaaacc aggaggaaaa ggaatagccg tttcatcttt
      781 ttcatgaaaa agggaaaaga aaaatgccgg cgtatttcac cggcattcat tcataagatc
      841 agcggacaga aacccgtgcc gtcatgcgat gtccgtcggc aagacgaacc tgcacgaggt
      901 aaaggccatc gggcattccc gtaagatcga gatcagcttt ggtttcacca ttggtgaata
      961 cgcggctgga gaccagcgcg ccgagcgtgt tgtacacttc gcagctttcc attttcgttc
     1021 ccgcgtcgat ggtgaaatga ccatcattcg ggttcgggaa aacggtgagc gtcaccatgg
     1081 attttttgat ctcgctgata cccgtcgggc aggaacaatt gtgcatgccg agatttgacc
     1141 agttgttttt cggaagcgaa tcccattcgg tggtaggatt gaaagccgta ggattcaccg
     1201 ttacaccttg tgtaaccgaa gacttgcgga tgagcgaatg gtctttggtc cagtaagcgc
     1261 cggccgcatc tgtgtacgga aaaacatccg tccagctctg gcccggatct tcaccgatct
     1321 tgccgtagat atcgacaaac gtgcgcggtg aaattttagc aagaacgatc gcgtcgttcc
     1381 cgttctggta acagggatcg ggataagcac cgccgagctg gtcggccatg ttctgaagcg
     1441 cagtatcaca agccggggaa gtcggtgtgg aagtcgtttg tccgttcgcc accacccaaa
     1501 cgtcgtgcgg agcgatagta ccactgaggg taaggctgtc cgtcggaacg gttgatccgt
     1561 tggaaaagcg gattatccgg taattcgaca atgcgatggg cgcattcgtc gggttgtaga
     1621 tttcgaaagt tttgttattc ccggatcctt cgtcgatctc ggagaaaaac agttcgctgc
     1681 attgagcatt ggcaaatacg ataaagccca acgacaggat cgtgagtaat tgttttttca
     1741 tttggtgatt ggtggaggaa ctgtgtgtat tctgattctg cactggtttc ggcttcgcac
     1801 ctttttcaag gcctgcctgt aggatatcaa tacagaactg cggcaaaaat aggatgttcg
     1861 ccctgccgtg tcaagccaaa acggccattt gtgaagaact tcagaatttt ttaattgggg
     1921 ctttaccgtt ccgaaatata gaggcgcgac tgggcgcgca tttgagcggc agaccgtaaa
     1981 aaaactactt ttgcacgtgt ttttaacaaa atcttccaat cgttatgaat aaaggtccca
     2041 tttcccagtt catcgaccat cattaccgtc acttcaacgc agcagcgctg aaagatgcgg
     2101 ccaaaggata tgaaacccac cttctcgaag gcggaaaaat gctcgtttcg ctggccggag
     2161 ccatgagcac ggcggagctc ggcatatcgc tcgcagaaat gatccgccag gataaagtgc
     2221 acatcatctc ctgcacgggc gccaacctcg aggaagacct gatgaacctc gtggcccatt
     2281 cccattacaa acgcgttccc aattaccgcg accttacgcc gcagcaggaa tgggacctgc
     2341 tcgagcaggg actgaaccgc gtcacggata catgtattcc cgaagaagaa gcattccgcc
     2401 gtttgcagaa gcacatttac gagctctgga aaaaagcgga tacgaacggc gaacgttatt
     2461 tcccgcacga gttcatgtac cagatgatca acagcggcgt gctgaagcag tattacgaga
     2521 tcgatccgaa gaattcatgg atgcttgcgg cggcggaaaa aaatctcccg atcgtcgtag
     2581 gcggatggga agattccacg atgggaaata ttttcgcgag ctacgtcatc aaaaaagaag
     2641 taaaggcgac gaccatgaaa agcggcatcg aatacatggt gtggctgacg gaatggtacc
     2701 gcgccaattc aggcggcaag ggcgtgggat ttttccagat cggcggcggc attgccggcg
     2761 acttcccgat ctgcgtggtg ccgatgatgt accaggatct cgaatggcac gatgttccgt
     2821 tctgggcgta tttctgccag gtctccgatt cgaccacatc gtacggatcc tattcaggcg
     2881 ccgtgccgaa cgaaaagatc acatggggaa agctcggcat cgacacgccg aaattcatcg
     2941 tggaaagcga tgcgacgatc gttgctccgc tcatcttcgc gtggatactc gggtggtaac
     3001 gacccaaatg agaattataa atgctgaact tttgactcag agccggcttg tcccgggtaa
     3061 acggcaggcc ggctttttcg ttgatcaatt cttccgtatt ttcacctgat ggccaaagca
     3121 aaagccgctt ccaagaacat tcccgtacaa acctccttct ggcagaacga ccgcaacacc
     3181 gggttcgcgc tcgccgcgtt cgccttcctg ctctatgcgc agacgatcag ctacggtttt
     3241 gtgctggacg atgtggccgt cgtgcacgga aatgaattcg tgaaaagcgg cttcagcggt
     3301 ttcggaaaaa tcctcactac attttactgg catggtttcc cgaatttcgc cacggcaaac
     3361 tcgggacttt tccggccggt ctcgctgctg ctctttgcca ccgaatggca attgttcccc
     3421 ggcaagccgc acgtttttca tttcgttcag gtcgcgctct atgcgatctg cgcgtaccag
     3481 ctttaccgtt tgctgcgtga attgctgcgc gaccattccg gctcgctggc actgatgacg
     3541 acactgctgt ggatcgtgct tcccgtgcat accgaggtag tggccaacat caaaagcgcg
     3601 gatgaagttc tttcgctgct tttctttttg ctttccttcc gtaagatcct cgcgtggagc
     3661 gaaaccggga aaacagcaac actcgtttct tccgccgggc tcatttttct ttcactgctt
     3721 tccaaggaag gtgcagtgat gttcgttccc gtcatgctga tcgcgctgat gcagttcagg
     3781 aacaaaaccg cgaagcagct ggtgctgccg gcgggcgttt ttcttgccac aggcattgtg
     3841 tggttggtgt ggcacagctg ggtgatctcg cacgcaggat cggcgatgat cacgtacgat
     3901 taccgcaaca acgcgctgct ttcctccact tccgtcgctg accggacagg aacagccatc
     3961 ggcatgcagg cacgttactg gatgaagatg ctggccgggt tcccgctttc gtacaattat
     4021 tccttcaatg aaattcccgt agatggattt gcgggtgtgt ggacgtggat cgcgctggcc
     4081 gggattttcg ccgccggttt tttcgcgtac aaaaatttca ggaagaaccc ggtcgtttcg
     4141 ttcagcctgc ttttttattt catcacgttt gcgctcacct gcaatatttt ctatctcatc
     4201 ggggaaacgt tcgcggagcg gcttgcattc gttccttcca tcggtttttg cctgctgttc
     4261 agcttccttg tgctccgttt caccaacggt ttgcgtgaaa aaagaataca tgcgcaggcg
     4321 atgtatgtgc tcgttccgct ggcgctcgtt tacagcatac gcaccttctc gcgcagccag
     4381 gtgtggacag acgaacccac gctctacacg aacgacgtag acaatgcacc gaacagcgcg
     4441 cgtgttcacg acaacagcgg cgtgctgctg tataatgcgg cgctgggaac gcaggacccg
     4501 aaccagaaga aacaattgtt cgggcaggct tattcggaat tcagcactgc ttacacgatc
     4561 gactccgtgg atttccaggc ggcacatatt cttgcagaga tcgaattcaa ccgcgggaac
     4621 aatgaagaag cgatacgttg gggaagaaag aatattgcct gcttccgctt ttttcacgtt
     4681 ccgcccaatg atccgaccgt gtattctttg atcgggaatg cgttcatgaa ccttcagcaa
     4741 tacgatagcg cacatgccgt ttttgcaacc gccgcttccc aatacccggg gaacgaaagc
     4801 ttcacgattg cgctgggtgg aaattcgctg gcgcagaaag acacgacggg cgccatctcg
     4861 ttcttcgaaa aagctacgtc catcaatccg aaatcggtcg ctgcatggga caagctggcc
     4921 aatgtgcgcg gcatgcaccg cgactaccgg g
//
LOCUS       DASTSF010000571         2107 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_2003810,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000571 DASTSF010000000
VERSION     DASTSF010000571.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2107
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2003810"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..330)
                     /locus_tag="VFU15_16145"
     CDS             complement(<1..330)
                     /locus_tag="VFU15_16145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719377.1"
                     /translation="MPAALIAQQASGQEANSTIFSNGVFLVMLTTIVLLLFVIKGMAE
                     VVKAGAAHRARAEKEKSKNAQKIAAVLFFVLAGKMLYAQNAGPAPEFTYWRMGAPLFF
                     LMLTIIVF"
     gene            complement(400..600)
                     /locus_tag="VFU15_16150"
     CDS             complement(400..600)
                     /locus_tag="VFU15_16150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014679705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcoQ/FixQ family Cbb3-type cytochrome c oxidase
                     assembly chaperone"
                     /protein_id="HEU4719378.1"
                     /translation="MKFIDYLTSITGVGIFPMISLMVFFFFFLGLGIYVWKADMNRFR
                     YLAALPVEENGESKPEKNTPHQ"
     gene            complement(606..>2107)
                     /gene="ccoO"
                     /locus_tag="VFU15_16155"
     CDS             complement(606..>2107)
                     /gene="ccoO"
                     /locus_tag="VFU15_16155"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015027226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit II"
                     /protein_id="HEU4719379.1"
                     /translation="YLGLMYYFVPKAANRPVYSYRLSIIHFWALIFIYIWAGPHHLLY
                     TSLPDWAQSLGTVFSIMLIAPSWGGMINGLLTLRGAWDKVREDVVLKFLVVAVTAYGM
                     ATFEGPMLSLKNVNAISHFTDWTIAHVHVGALGWNGFLTFGILYWLIPRMFRTQLYSR
                     KLANTHFWLGTLGILFYAVPMYWAGFTQSLMWKEFNPDGTLANKFLDTVTHLAPMYAM
                     RSLGGVLYLSGVIIMTYNIIKTVKAGSFVANEEAEAPALAKNYVIHGKEHWHRWIERR
                     PVQMLILALVLVAVGGIIEFIPTFLVKSNVPTIASVKPYTPLELEGRDIYIREGCYNC
                     HSQMIRPFRSEVARYGEYSKAGEFIYDHPFQWGSKRTGPDLARIGGKYGDAWHYNHMF
                     DPQSMSPGSIMPSYPWLFDNKIDFSGTAAKIRAMQTLGVPYPDGYDKIAADDARKQGQ
                     AIVDNLEKAKVQAVAPLSPDNEIVALIAYLQRLGTDIKAKPSPGKTAQK"
BASE COUNT          500 a          545 c          567 g          495 t
ORIGIN      
        1 gaaaacgatg atcgtcagca tgaggaaaaa aagcggtgcg cccatgcgcc agtaggtaaa
       61 ctcaggggcc ggtcctgcat tttgagcata cagcattttc cctgcgagaa cgaagaagag
      121 caccgccgcg attttctgcg cattcttcga tttttccttt tctgcgcgcg cgcgatgcgc
      181 cgcaccggcc ttcaccactt ccgccattcc cttgatcacg aaaagaagaa ggacaatggt
      241 cgtcagcatg acgaggaaca caccgttgga aaagatggtg gaattggctt cctggccgga
      301 agcctgctgc gcgattaacg ctgccggcaa aataaccgcg cttattattg ccgtctttct
      361 tttatggttg aacgtgttca tacagattcg tttgaatcgt tattgatggg gtgtgttctt
      421 ttccggtttg ctttctccgt tctcttccac cggcagggcc gccaggtaac ggaaccggtt
      481 catgtctgct ttccacacat aaataccgag gccgagaaag aagaaaaaga aaaccatcag
      541 tgagatcatc ggaaaaattc cgacgccggt tatggaagtg agatagtcga tgaatttcat
      601 ggcgtttatt tttgtgcggt tttaccaggt gatggttttg cttttatgtc cgttccgaga
      661 cgctgcaggt aagcgatcag tgcgacgatc tcgttgtcag gagaaagcgg cgctactgcc
      721 tgcactttcg ctttttccag gttgtcgacg atcgcctgtc cctgcttgcg cgcatcatcc
      781 gctgcgatct tgtcataacc atccgggtaa ggaacgccca gcgtctgcat tgcgcggatc
      841 ttggctgccg ttccgctgaa atcgatcttg ttgtcgaaca gccacgggta agaaggcatg
      901 atggatcccg gcgacatgga ttgcggatcg aacatgtggt tgtaatgcca tgcgtcaccg
      961 tatttaccgc cgatgcgtgc aagatcagga cctgtccgtt tcgatcccca ctggaaagga
     1021 tgatcgtaaa tgaattcccc ggctttggaa tactctccgt aacgggccac ttcggaacgg
     1081 aacgggcgga tcatctgcga atgacaattg tagcagcctt cgcggatgta aatatctctt
     1141 ccttcgagct ccagcggagt atacggtttc acgcttgcga tcgtgggaac atttgatttc
     1201 acaaggaacg taggaatgaa ttcgatgatg ccgccgactg caacaagcac gagcgcgagg
     1261 atcagcatct gcacaggacg gcgttcgatc cagcggtgcc agtgttcttt tccgtgaata
     1321 acataattct tcgccagcgc cggggcttcc gcttcttcgt tcgccacgaa ggatcctgct
     1381 tttacggttt tgataatgtt gtacgtcatg atgatcacgc cgctgaggta aagcacgccg
     1441 ccgagtgagc gcatcgcgta cataggcgca aggtgcgtga ccgtatccag gaatttattc
     1501 gccagcgttc cgtccggatt gaactctttc cacatcaggc tttgcgtaaa tcctgcccag
     1561 tacatgggaa cagcatagaa aagaatgccc agcgtgccga gccagaaatg cgtgttcgcc
     1621 agcttgcgtg aatacagctg cgtacggaac atgcgcggga tgagccagta taaaataccg
     1681 aacgtgagga aaccattcca gccaagcgct cccacgtgca cgtgcgcaat cgtccagtcg
     1741 gtaaagtggc tgatcgcatt cacgttcttc agcgaaagca tcgggccttc gaacgtcgcc
     1801 atgccataag cggtaacggc gaccaccagg aatttcagaa cgacatcttc gcgcacttta
     1861 tcccatgcac cgcgaagtgt caggagtccg ttgatcatcc cgccccagga cggcgcgatg
     1921 agcataatgg aaaatacggt tcccagcgat tgcgcccagt cgggaagcga tgtgtaaagc
     1981 aggtggtgcg gaccggccca gatgtaaata aagatcagcg cccagaagtg aatgatggaa
     2041 agccggtacg aataaaccgg ccggttcgcc gctttcggaa cgaaataata catcagtccg
     2101 aggtacg
//
LOCUS       DASTSF010000572         6072 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1898667,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000572 DASTSF010000000
VERSION     DASTSF010000572.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 6072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6072
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1898667"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            114..434
                     /locus_tag="VFU15_16160"
     CDS             114..434
                     /locus_tag="VFU15_16160"
                     /inference="COORDINATES: protein motif:HMM:NF025092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEU4719380.1"
                     /translation="MKKAAIYLFILLFLPAITFANPGDGAAFINGKVTDKDTHQTLAG
                     VEVHVKGTNITTYSDFDGNFFISDLPAGSYELEFHFITYDETSVVTSKTTGQQSITVE
                     MQQR"
     gene            603..1073
                     /locus_tag="VFU15_16165"
     CDS             603..1073
                     /locus_tag="VFU15_16165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HEU4719381.1"
                     /translation="MKRVMMILLLCAGVFALHAQEVSFKNGMYYTADGKLFSGQFTQF
                     FTNGAKKCDVAVNNGKANGTAVYYFESGNKKESGNFVDGLRDGMWESWNDAGVKTTEA
                     NYKMGKKDGQWIVWDDAGNKRMEMHYTDDQKTGTWKMWDDKGVQTGERKYSDAQ"
     gene            1261..1998
                     /locus_tag="VFU15_16170"
     CDS             1261..1998
                     /locus_tag="VFU15_16170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HEU4719382.1"
                     /translation="MKARFIFIALMAGIAFSACKKDEISSGPRAITPHDFLSGDDYKS
                     LTVEVCYVTGHQPTNEALNAVMDMLSARLNKPEGIQIAYHEIASPRLSSFTTDDLKRL
                     DKSVRDHYTKGKKLAVFVFFADAPYAESSGNYQVLGLAYGTTSMAIFEKTVEDYSNGL
                     GEPSRYVLEATVCEHEFGHLLGLVDNGTPMVSAHLDAAHPHHCDDHNCLMYYTTETTD
                     ILSNLTGGNIPVFDDNCLADLKANGGK"
     gene            complement(2076..3962)
                     /locus_tag="VFU15_16175"
     CDS             complement(2076..3962)
                     /locus_tag="VFU15_16175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEU4719383.1"
                     /translation="MNILSVENISRSYGDRVLFRDISFGLSDGEKVALVARNGAGKTT
                     LLNILAGKDQPDSGRVSVRKDITIGFLKQDPQFAPGHTVIEAALGGDNEISSVVREYE
                     LVSEKAAADPSVHRRLDELIAKMDSLEAWDHETKVKALLSRLGFTQLSQTAESLSGGQ
                     KKRLALAQQLIASPQLLILDEPTNHLDLDMIEWLENYLISKDITLLLVTHDRYFLDRV
                     CDRIIEIDNGKLFSYEGDYSYFVEKKSEREEAESGALDKARNLYRRELAWVRKMPKAR
                     GTKAKARKDAFRTVAEKAKGRKPEEELKLEVKMTRLGGKILELIRISKNFGAKIILKS
                     FDYTFRKGEKVGIIGKNGSGKSTFLNILLGLEKYDAGKIQTGETVVFGYYSQDGMITR
                     EDKRVIEVVKEIADVFPLADGSNLSASQMLQRFLFPVNTHYNYVSALSGGERRRLYLL
                     TVLMKNPNFLVLDEPTNDLDILTLSVLEDFLSDFPGCVIVVSHDRYFMDRLVDHLFVF
                     EGDGVVNDFPGNYSQWRERQDEAKPEKKNADVAPAEEKKTAANNSSQGKRKPSFKEKF
                     EFDNLEKEIPSLEKEKSEITQKLSTVSDHVELQKLSTRFSDIEAELDQKTLRWMELAE
                     IMEA"
     gene            complement(4050..4865)
                     /locus_tag="VFU15_16180"
     CDS             complement(4050..4865)
                     /locus_tag="VFU15_16180"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEU4719384.1"
                     /translation="MASLLIRIYDVKKNNHTVLLRELFPFTFVVLLWIIQLVQAFLQT
                     SWADYSLYPRSVHGLIGIFTAPLLHANFGHLTGNTIPLLFLGYLLFNSYREIAGKIFL
                     MIYFLTGILDWFLARSAFHLGASGVVYGLATFLFFSGLIRKNPPLMVISLLVVFLYGS
                     LVWGVFPIDPQVSWESHLYGALTGAILSVVYRREGPQPKKYFEDEKEEEENGLPENFN
                     PGVQHHGTHNPQMTQEAEPFIPADPLFPRVIIRYDYTEKKKGEGNEKKEEDEK"
     gene            4975..5190
                     /locus_tag="VFU15_16185"
     CDS             4975..5190
                     /locus_tag="VFU15_16185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719385.1"
                     /translation="MSNGEIAQIFLKNGRRETGLLLNDVNRENAFDEGVKYVPNRSIG
                     AWLDSFSQDLVQVIHPDQVEGIDLFLR"
     gene            complement(5221..>6072)
                     /locus_tag="VFU15_16190"
     CDS             complement(5221..>6072)
                     /locus_tag="VFU15_16190"
                     /inference="COORDINATES: protein motif:HMM:NF019284.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719386.1"
                     /translation="TIVYSPSGAPIIQTVFEDGVQHGLRREFYDSGKPFIETHYAYDV
                     KDGTCNAWWENGNKRWEENYYDGELHGDQNYYYENGQLSRTGKWEDGYLNGELKIYAD
                     DGALAMIRYYRYGTFIGYSYLGKDGSKVEMEKLEDASGKFTTYFQNGNKAVSGKYEYG
                     KLDGKYFMYYSDGQVEREENYDYGDLKGVQKYYYHNGNVKKELNYYGDVLDGVQKYYD
                     ENGKLEHTEVYALDTRWGTWKYYDADGNVKSTKTYYNGEIIAQYTAPAPAPSPAANTP
                     KPKGKGK"
BASE COUNT         1412 a         1605 c         1511 g         1544 t
ORIGIN      
        1 atgaaaaatc cacaaggaga cattccggat ttacaaatcc ttcatattac cgtaattttt
       61 ttcaaacagg gtccaccgcc ttccgggact atttttgccg ggttcattca cccatgaaaa
      121 aagcagcgat ctacctcttt attttacttt ttttgccggc aattactttt gccaatcccg
      181 gagacggcgc ggcatttatt aacggaaaag tcacggacaa agacacgcat caaacccttg
      241 ccggtgttga ggttcatgta aaagggacga acatcaccac gtattccgat ttcgacggga
      301 actttttcat ttccgatctt cctgcgggtt cctatgagct cgaattccac ttcatcacct
      361 atgatgaaac ttcggtcgtc acttcgaaaa caaccgggca gcaatccatt accgtggaaa
      421 tgcagcagcg ctgattcctt ttcccaatat taaattttac attcccgtaa tactttatca
      481 cactggcttc caattatttg cattcattgt aaagttaacg ttaccgtatc attagccaaa
      541 tgaaacctgt gtcacgaatt ttgtaattgt agataaagtg ttcattctaa aaaacaacgg
      601 gtatgaaacg ggttatgatg attcttcttc tctgtgcagg cgtattcgcc cttcatgcgc
      661 aggaagtcag ttttaagaac gggatgtact atacggctga cgggaaactt ttttccgggc
      721 agttcacgca gttttttaca aacggtgcga agaaatgcga cgttgccgta aacaacggga
      781 aagcaaacgg aactgctgtt tactacttcg aaagcgggaa taaaaaagaa tccgggaatt
      841 ttgtggatgg cttgcgcgac gggatgtggg aaagctggaa tgacgcaggc gtcaaaacaa
      901 cggaagctaa ttataagatg ggtaaaaaag acgggcaatg gatcgtgtgg gacgatgccg
      961 ggaacaagcg catggaaatg cattacaccg acgaccagaa gaccggtaca tggaaaatgt
     1021 gggatgataa aggcgttcaa accggtgaac ggaaatattc cgatgcgcag tgacgcgctg
     1081 aagtcaacct ggaaataagg aaggtcgccg gtggcggcct tttctttttt ttagcgcacg
     1141 cgttttttct gcaaagcgta gaatgagtcc tgcgatgcgt cgaaaacaaa aaaccggcgc
     1201 cggcccggga gcgtattttt gatcgtggca gaagttcaac gcgttcaaaa attcacgacg
     1261 atgaaagcac gatttatttt cattgcgctg atggccggaa tagctttcag cgcctgcaag
     1321 aaagacgaga tcagcagcgg cccccgggcg atcacgcccc acgattttct ttcgggggac
     1381 gattataaaa gtcttaccgt ggaagtctgt tatgtgacgg gacatcagcc gaccaacgaa
     1441 gcgctgaatg cggtgatgga catgctgagc gcaaggctca acaaaccgga agggatacag
     1501 atcgcttacc acgagatcgc ttcgccgcgg ttgtcttctt tcacaacgga cgacctgaag
     1561 cggcttgaca aaagcgtgcg cgatcattac acgaaaggca aaaaactggc ggtatttgtc
     1621 ttttttgcgg atgcgccgta cgctgaaagt tccggcaatt accaggtgct cggtctcgct
     1681 tatggcacca cctccatggc cattttcgaa aaaacagtgg aagattattc caacggtctc
     1741 ggggaacctt cccgctacgt tctcgaagct acggtgtgtg aacacgagtt cggtcacctg
     1801 ctcgggctgg tcgataacgg cacacccatg gtgagcgctc acctcgatgc ggcgcatccg
     1861 caccattgcg acgaccacaa ttgcctgatg tattacacga cggaaacaac cgatatcctc
     1921 tccaatctca cgggcggcaa cattccggtg ttcgatgata attgtcttgc agacttgaaa
     1981 gccaacggag gaaaatagaa ccgcatcctt tttcctttac ctcactatgc aggacaatgg
     2041 ggagagccgg cgcaggatct gcgccggcct gttttttacg cttccatgat ctccgccagc
     2101 tccatccagc gcagcgtttt ctgatccagc tccgcttcga tatctgaaaa acgcgtggac
     2161 agcttctgca gctcgacgtg gtcggaaacg gtggaaagct tttgcgtgat ctctgatttt
     2221 tctttttcga gcgaagggat ctctttttcg aggttatcga attcgaactt ttccttgaaa
     2281 gaaggtttcc gttttccctg tgacgaatta ttggccgccg tttttttctc ttcggcggga
     2341 gcaacatccg catttttctt ttccggcttt gcttcgtcct gcctttctct ccactgggaa
     2401 taattccccg gaaaatcatt tacgacgcca tcgccttcga acacgaataa gtgatccacg
     2461 aggcggtcca tgaaataacg gtcgtgggaa acaacgatca cgcagcccgg gaaatcagaa
     2521 aggaaatcct cgagcacgct gagcgtaagt atgtcgagat cgttcgtcgg ctcgtccagc
     2581 acgaggaagt tcgggttctt catcagcaca gtaagcaggt acagtctccg ccgttctccc
     2641 ccgctgagcg cggaaacgta attgtaatgc gtgttcacgg gaaaaaggaa acgctgcagc
     2701 atctgcgatg cggaaagatt gcttccgtcg gcgagcggga acacgtcggc gatctccttt
     2761 accacttcga tcacccgctt gtcttcccgc gtgatcatgc cgtcctgcga ataatagccg
     2821 aacacgaccg tttcaccggt ttgtattttc ccggcgtcgt atttttcaag accgagcagg
     2881 atgttgagga acgtcgattt cccgctgccg ttcttcccga tgatccccac cttctctcct
     2941 ttccggaacg tgtaatcgaa gcttttcagg atgatcttcg cgccgaaatt tttcgaaatg
     3001 cggatgagct cgaggatctt cccgccgaga cgtgtcatct tcacttccag cttcagctct
     3061 tcttccggct ttcgtccttt cgccttttcg gcaacggtcc tgaacgcatc tttacgcgcc
     3121 ttcgcttttg ttccccgcgc tttcggcatt ttacgcaccc aggcaagctc gcgacggtaa
     3181 aggttgcgcg ccttatccag cgcaccggat tccgcttctt cccgttcgga ctttttttcc
     3241 acgaaatagc tgtaatctcc ttcgtaggaa aacagcttcc cgttgtcgat ctcgatgatg
     3301 cggtcgcaga cacggtcgag gaaatacctg tcgtgcgtca cgagcagcag cgtaatgtct
     3361 ttcgagatga ggtaattctc gagccactcg atcatgtcga gatcgaggtg gtttgtcggt
     3421 tcgtcaagga tgagcagctg cggggaagcg ataagctgct gggcaagcgc cagtcttttt
     3481 ttctgcccgc cggaaagcga ttccgccgtt tgcgagagct gcgtaaaacc gagacgggac
     3541 aacagcgcct tcactttcgt ttcgtgatcc cacgcttcga ggctgtccat tttcgcgatc
     3601 agctcgtcga gccggcgatg aacggacgga tcggctgcgg ccttctcgga cacaagctcg
     3661 tattcacgca ccacgctgga aatctcattg tcgccaccga gtgccgcttc gatcaccgtg
     3721 tgcccgggag caaactgcgg atcctgcttc aaaaacccga tggtgatgtc tttccgtacg
     3781 gaaacccgcc cgctgtccgg ctggtccttc ccggcgagga tgttgagcaa cgtcgttttg
     3841 cctgcaccgt tcctcgccac gagcgccact ttttccccgt ccgaaagacc gaaggaaatg
     3901 tcacggaaca gcacacggtc gccatagctg cgggatatat tttcaacaga aagaatattc
     3961 atcgcgcaaa actacgaata caaatggcgt aaaatggagt ttcttcccgc cgttcttcag
     4021 cacgggcgcg cctttcacga cggccccttt cacttttcgt cctcttcctt tttttcattc
     4081 ccctctccct tcttcttttc agtgtaatca taccggatga tcacccgcgg gaaaagcggg
     4141 tcagcaggga tgaatggttc cgcttcctgc gtcatctgcg ggttatgtgt tccgtgatgc
     4201 tgtacgccgg gattgaaatt ttccggcagc ccgttttctt cttcctcttt ttcatcctcg
     4261 aaatactttt tcggctgcgg accttccctg cggtaaacca cggaaagtat cgcgccggtg
     4321 agcgccccgt agagatgcga ttcccacgat acctgcgggt cgatcgggaa cacgccccac
     4381 acgaggcttc cgtacaggaa aacgaccagc agcgagatga ccatgagcgg aggatttttc
     4441 cggatgagcc cgctgaaaaa aaggaatgtg gccagtccgt aaacgacccc gctggccccg
     4501 agatggaatg cgctccgtgc aaggaaccag tcgagtatgc cggtcaggaa atagatcatc
     4561 aggaaaattt tcccggcaat ctcgcggtag gaattgaaaa gcaggtagcc gagaaaaagc
     4621 agcgggatcg tgttgcccgt caggtgaccg aaatttgcat gcagcagcgg cgcggtgaag
     4681 atgccgatga gcccgtgcac gctccgcgga taaagggaat aatccgccca cgacgtctgc
     4741 aggaacgcct gtaccagttg aatgatccag agcaatacga cgaaggtgaa cgggaaaagc
     4801 tccctgagca ggacggtatg attatttttt ttcacgtcgt atatgcggat caatagggat
     4861 gccacggtgg gttttcatgc ctatctggca gcatttgatc agattccctt tatatccgct
     4921 taacggtggc ttaaaactgt ctccggattt ttgtatgaac taaaaccaag aattatgagt
     4981 aacggagaaa ttgcacagat cttcctgaag aacggccgcc gggaaaccgg gctgctgctg
     5041 aatgacgtga acagggagaa tgcattcgac gaaggtgtga aatatgttcc gaaccgcagc
     5101 atcggggcat ggctggattc cttttcgcag gacctcgtgc aggtgataca tcccgaccag
     5161 gtggaaggca tcgacctttt cctcagataa aaaaagcggg cgctctgccc gcttcttttt
     5221 ttatttccct tttcctttcg gtttgggtgt gttggcagcc ggcgacggcg caggagcagg
     5281 tgcggtgtac tgcgcaatga tctccccgtt gtagtaggtc ttcgtcgact tcacgttccc
     5341 gtccgcatcg taatacttcc acgtgcccca ccgcgtatcg agcgcataca cttcggtatg
     5401 ctcgagcttt ccgttctcat cgtaatactt ctggacaccg tcgagcacat cgccgtaata
     5461 attcagttcc ttcttcacgt tcccgttgtg ataatagtat ttctgcacgc ccttcaggtc
     5521 gccgtagtcg taattctcct cgcgctccac ctgtccatcg gaataataca tgaaatactt
     5581 tccgtcaagc tttccgtatt catactttcc gctgaccgcc ttgttcccgt tctggaaata
     5641 cgtcgtgaat tttccggatg catcctccag cttttccatc tcgaccttgc tgccatcttt
     5701 tccgagatac gaatacccga tgaacgttcc gtaccggtaa taacggatca tggcaagggc
     5761 gccgtcatcg gcataaattt ttaattcgcc gttgaggtag ccgtcttccc atttaccggt
     5821 gcgggaaagc tgcccgttct cgtaataata attctgatcg ccgtgcagct cgccgtcgta
     5881 atagttttct tcccaacgct tattgccatt ttcccaccac gcattgcagg tcccgtcctt
     5941 cacgtcatag gcgtaatgcg tttcaatgaa aggcttgcct gaatcgtaga attcgcggcg
     6001 caggccgtgc tgaacaccgt cttcgaatac cgtctgtatg atgggagcac cggaaggtga
     6061 ataaacgatc gt
//
LOCUS       DASTSF010000573        13367 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1452940,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000573 DASTSF010000000
VERSION     DASTSF010000573.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 13367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13367
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1452940"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..268)
                     /locus_tag="VFU15_16195"
     CDS             complement(<1..268)
                     /locus_tag="VFU15_16195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014682517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEU4719387.1"
                     /translation="MFKETVDFIRAHYGTDKFIPLHEPRFAGKEKEYLIDCIDSTFVS
                     SVGEYVDRFEKTMAEYTGAKYAVAATNGTLGLHMALIVAGVKRGD"
     gene            complement(261..1259)
                     /locus_tag="VFU15_16200"
     CDS             complement(261..1259)
                     /locus_tag="VFU15_16200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014682516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent 4,6-dehydratase LegB"
                     /protein_id="HEU4719388.1"
                     /translation="MDLKNRQVLVTGADGFIGSHLVEKLLDEGCRVRAFVCYNSFNSW
                     GWLDTFPKELLSRIEIFSGDIRDPNGVRTAMKNVEVVFHLAALIAIPYSYHSPDSYVD
                     TNIKGTLNILQAARDCGVKKILSTSTSEVYGTAMYVPIDEKHPKQGQSPYSATKIGAD
                     HIAESFYRSFDLPVTIVRPFNTFGPRQSARAVIPTIITQLLAGMKEIKLGAIHPTRDL
                     LFVKDTAAGFAAIAKCDNVAGEEINLCTQQEISIGDLAQKLISKINPAAKIVTDDVRL
                     RPEKSEVERLLGSNEKIKRLTGWSPENSFDAALGQTIEWFRSPENLARYKADIYNV"
     gene            1364..1612
                     /locus_tag="VFU15_16205"
     CDS             1364..1612
                     /locus_tag="VFU15_16205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719389.1"
                     /translation="MKNRTLLFLVLIIFCREGPENAGFIPNVSHRCQAMRMEKGPERL
                     LQPFERVRATGIKMAAGKYPGYLYPAIHGKTSGGILNP"
     gene            1617..2981
                     /locus_tag="VFU15_16210"
     CDS             1617..2981
                     /locus_tag="VFU15_16210"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HEU4719390.1"
                     /translation="MKNITHSVFIIALLGSFFPKNASAQCTAFFTWTQTANNTITFTD
                     ASTGNCGVPMRQWAFGDMSSGFGGNPVHFYSAPGNYIVTLIVTDSISSSWQTQFTDTI
                     TVTGTVFCNLSSWIGISQHPTCSTCADGALYAGSNNGTAPFTYLWSTGAVTPMISNLN
                     PGFYSVCVTDANNCQVCDTLTLDTVPYYSSCIPSFTHTQSQPNVVDFSGSTILTQPMW
                     GWSFGDNSGGYGPNTSHTYAYTGVYNVCLSVWDSLSGCSGTYCDTVSVWGNNASCQGS
                     FYSYYDSLVPNTAWVVNQSVGAQPISCTWYWGDNTFDTIQYPSHTYTVSGTYSICLVI
                     HDNAGCTDSVCQNIAVYRMAYSSASVPFYVNVVGPTGIRESSQQSWSLFPNPAENEIR
                     IKTDYSLNGKQYRIVDISGRILVSSRLDGNRIDISSFDKGIYFLQIENGDNGAFSSQR
                     FIKN"
     gene            complement(3058..5505)
                     /locus_tag="VFU15_16215"
     CDS             complement(3058..5505)
                     /locus_tag="VFU15_16215"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEU4719391.1"
                     /translation="MKKLLTAAALFFAVAAGAQFVGNGSFTGAKRTYLDDGAWVPREH
                     NVDFIHMTLDVSFDVHAGLVKGKVIHRFTPLQKAVDSIWVDGPGIRILEASVNGKQVT
                     YRTLDQGTWIKMPKTLTWNETDSMTLVYEANPRHGLYFVGWNDPAGICRKQVWSQGQG
                     IDNRNWIPFYDEMNDKITTDMFVTFDSQYKVLSNGDKVEEKDNKDGTKRWHYRLSKPY
                     APYLVMLGIGIYDIKETKSKSGVPMHLYYYPDWKDRVDVTYQYSEQMIDFYESEIGVK
                     YPWPSYSQIPVQEFMYGAMENVTATVYGDFLFVDDRSNWDRGYVGVNAHELAHQWFGD
                     YVTAKSDAHHWLQESFATYYDQLFERSIWGDDHFNWQRREAENAALDEGKKNDLPIAH
                     SQAGSARHYPKGAFVLNMLKYVVGSREAYNRGIKYYLDHHAYSNVTSDDLLNGFNESL
                     GLSLNWFWEEWVYHGGEPSYDVDANDLTDNISGRHFTRFNVKQVQQRSDVVGLFRMPI
                     WFEVHYADGTSDRVQQWIEKETEIVDIPNPSGKKISYVLFDPNNEVLKTVSFKKSFDW
                     LKNQALNAPNMLDRYDALVALRDYPLEQKREVLKQVYAKETFQATKGEIVAQLINDTN
                     PDSRSIIRSAINDKDVLMHRNVINNTTMVAADMEGDYEKLLSSPSYEVVMSTLDKLSG
                     LFPDKAAKYLDMTKGVIGTSGRNVEVKWLEINARTTGDKASIDRLVALTNMSYEFRTR
                     VNAAQALKRLDYFDESLMKNLVDASFSANTRLANPCDEVLLYFYNQDKNRKTIRDYAI
                     GQTWKDWQKNILDGLLN"
     gene            5690..7024
                     /locus_tag="VFU15_16220"
     CDS             5690..7024
                     /locus_tag="VFU15_16220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013928727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEU4719392.1"
                     /translation="MGNFSILIIDDEPKLRSLLARMLELEGYPVSQAENAGKGWEYLK
                     THPHTSLVLLDVRLPDENGISFLEKLKAGYPGTEVIVMTAYGTIRDGIKAMQLGAFDY
                     LVKGDDNDKLIPSVSRVKEKVLLQRRIENLENRIGEKFRFENILGTSMVIREAVRLAE
                     KVAPTDSTVLLEGETGVGKELFAQSIHSASPRSMQSFVAVNCSAFPKDLLESELFGHK
                     KGAFTGAAYDKKGLFQEADQGTLFLDEIGEMSLELQAKLLRVLETQTFMRVGDTKPVK
                     VNVRIIAATNRNLKNEGEAGSFRADLYFRLSSFVISVPALKNRPEDIEPIARHFLNHY
                     ASRVGKRVKGMDAEFLGRLKKHDWKGNIRELKNIIERAVILCQDEMLTTELLPEQFTN
                     AEDADDFTLATCEKKHILQVLRRTNGNKTKAAEMLGIGLTTLYRKIEEYKLY"
     gene            7202..8128
                     /locus_tag="VFU15_16225"
     CDS             7202..8128
                     /locus_tag="VFU15_16225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017494828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEU4719393.1"
                     /translation="MEAIVIGDKIARARKKTNLSQAQLAEKLFISPQAVGKWERGESV
                     PDIVTIGKLAKILGVDLNYFSDSSQSIEAETGHAGADDTTQTVNGSDSPSAERRLLTN
                     FNGSALAETDFTGVSAQKRKFIGSDLHGSDFSGADLTGSSFTGSDLHDADFGRTNLTD
                     CSFVALDLTNAGFNETILVRTEFSSSDLSGATFRNTKLVDVKLTTTDLKKTIFENCTF
                     SGVDFKSSDLSGLCLDGQTFTGVRFDNADLKNASFKGATLKNVSFRPSFALTNRYYKT
                     LKTISFDGAKMDKLTYAALKGVGVDLSKVTTI"
     gene            8294..8569
                     /locus_tag="VFU15_16230"
     CDS             8294..8569
                     /locus_tag="VFU15_16230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719394.1"
                     /translation="MDKKQISELILRNYYHTGGRTVRLTLADGSVHDGMFAGYYHDDI
                     DDGENPPDRWHFVTEKEFTDFSGAAGNRENFGIIIRTEEIVSVQFKT"
     gene            8853..8930
                     /gene="kdpF"
                     /locus_tag="VFU15_16235"
     CDS             8853..8930
                     /gene="kdpF"
                     /locus_tag="VFU15_16235"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HEU4719395.1"
                     /translation="MTALFIIGLGVFVYLVYVLIKPEKF"
     gene            8942..10642
                     /gene="kdpA"
                     /locus_tag="VFU15_16240"
     CDS             8942..10642
                     /gene="kdpA"
                     /locus_tag="VFU15_16240"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HEU4719396.1"
                     /translation="MNTEIIGIIFMYAVTLLLAIPLGKYIGKVFSGEKVWTDFMNPAE
                     RFIFRISGINPSQEMNWKQHLKAMLTINFVWFLLSMLVLMNMSWLPLNPDHNPSMSAD
                     LAFNTSVSFVTNTNLQHYSGETGLSYLGQLTLMLWQFISAATGTAICAVVFFALREKQ
                     NPGLGNFYNLLVKSTTRIFLPIAFVVAIMLLFSGTPMTLLGKDPVVTLQGDSTQVSRG
                     PVAAMVAIKQIGTNGGGYYGPNSAHPLENPTYFSNIVENISIILIPIAMVFAFGYIMR
                     RKKLALTIFAVMTAGFLCFLIPSVIVEMHGNHAMDHMHVLQSTGSMEGKEVRLGPAAS
                     AYWAINTTVTSNGSVNAMHDSMTPVTGMFTMLGMMVNSFYGGVGVGFLNYFIFIILAV
                     FISGLMVGRTPELLGKKIEAKEMKIAMIIALLHPLLILTGTAISSHLYANDPAKYSGW
                     LNNPGYHGFSEMLYEFTSSSANNGSGFEGLGDNTPFWNIACGLVMLLARFLPIIGPVA
                     IAGMLAKKKFIPQSAGTLHTDTATFGLMTFAVIMIVAALAFLPALTLGPLAEYFSLHH
                     "
     gene            10671..12743
                     /gene="kdpB"
                     /locus_tag="VFU15_16245"
     CDS             10671..12743
                     /gene="kdpB"
                     /locus_tag="VFU15_16245"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HEU4719397.1"
                     /translation="MSSVRKQAKAMKLFERKLVLQALKQSFVKLHPKFMVKNPVMFTV
                     EIGTAIMLAICIWVMTGEKSQGSPTYNWLIFSILLLTVLFANFAEAIAEARGKAQAES
                     LRKTREETPAKLIVPLGELYVNEVQKVSSSQLKKGDVFLCEAGDIIPLDGEIIEGIAT
                     IDESAITGESAPVIREAGGDKSSVTGGTKVLSDQVKVKVTVEPGESFLDKMIALVEGA
                     SRQKTPNEIALTILLAAFTLVFIIVCVTLKPFADYAQTPITIAAFISLFVCLIPTTIG
                     GLLSAIGIAGMDRALQANVITKSGKAVETAGDVDVLLLDKTGTITIGNRKATSFHPAP
                     GISGEKFMGDCVLASLADETPEGKSIIELAKSLGAKTHADESLMRDAKFIQFTAETRS
                     SGIELKNGIVVRKGASDAIANFAKKAGNAFPDETQKKVSEIASNGGTPLVVAENNKVL
                     GVIELQDIIKPGIRERFERLRKMGVKTVMVTGDNPLTAKFIAEKAGVDDFIAEAKPED
                     KMNYIKKEQAKGKLVAMMGDGTNDAPALAQADVGVAMNSGTQAAKEAGNMVDLDNDPT
                     KLIEVVEIGKQLLITRGTLTTFSIANDVAKYFAIVPALFIASIPALQGMNIMNLHSPE
                     SAILSAVIFNAIIIPILIPLALKGVKYKPIGASALLRRNLLIYGLGGVIIPFVGIKLI
                     DLFVGIFF"
     gene            12765..13325
                     /locus_tag="VFU15_16250"
     CDS             12765..13325
                     /locus_tag="VFU15_16250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009282071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HEU4719398.1"
                     /translation="MKPYLLPSLKLTLVLIVICSCLYPLLVAGIAKAAPGGGGGVTVS
                     VNGKVVGYENIGQKFDRDDYFWSRPSAVDYNAAGSGGSNKGPTNPDYLAQVQARIDTF
                     MVHNPDVNKNEIPVDLVTASGSGLDPDISIQAAKCQVPRIAKIRNMSEQDLYALIDKT
                     KQGPLLGFLGPEKVNVLKLNIALDAK"
BASE COUNT         3335 a         3397 c         3453 g         3182 t
ORIGIN      
        1 catcgccgcg cttcacaccg gcaacgatca gcgccatgtg aagacccagc gttccgttgg
       61 tagcggccac tgcgtatttc gcgcccgtgt attccgccat cgttttttca aagcggtcaa
      121 catattctcc cacggaggaa acgaaggtgg aatcgatgca gtcgatgagg tattcttttt
      181 ctttcccggc gaaccgcggc tcatgcagcg ggatgaactt gtccgttccg taatgcgcgc
      241 ggataaaatc taccgtctcc ttaaacattg taaatatctg ctttgtatcg tgcgaggttt
      301 tccggactgc ggaaccattc tatcgtctgg ccgagagctg cgtcgaatga attttccggc
      361 gaccagcctg tcagtctttt tattttttca ttgctgccga gcaaccgttc cacttcgctt
      421 ttttccgggc gaagacgaac gtcgtccgtg acgattttcg ctgcgggatt gatctttgaa
      481 atgagcttct gcgccagatc gccgatggaa atctcctgct gcgtacagag gttgatctct
      541 tcacccgcaa cgttgtcgca tttcgcgatg gcggcaaagc cggccgccgt gtctttcacg
      601 aagagcaggt cgcgcgtggg gtggatggcg ccgagcttta tttccttcat cccggccagc
      661 agctgcgtga tgatcgtggg aatgacggca cgggccgact ggcgcggacc gaacgtattg
      721 aacgggcgga cgatcgtcac cggcagatcg aagctgcggt agaatgattc ggcgatgtga
      781 tcggcaccga tcttcgtggc tgaatacggc gactgtccct gcttcggatg tttttcatcg
      841 atcggaacat acattgccgt tccgtacact tccgaagtgg atgtggaaag aatttttttc
      901 acgccgcagt cacgcgccgc ctgcaggatg ttcagcgtgc cttttatgtt cgtatcgacg
      961 tagctgtcgg gagaatggta gctgtacgga atggcgatga gcgcggcgag gtgaaacact
     1021 acttcaacgt tcttcatcgc ggtgcgcaca ccattcgggt cgcggatgtc gccggaaaaa
     1081 atttcgatac gagaaagaag ctctttcgga aacgtatcga gccagcccca ggaattgaac
     1141 gagttgtagc acacgaacgc gcgcacgcgg catccttcat ccagcagttt ttcaacgagg
     1201 tgactgccga taaacccatc ggccccggta acgagtacct gcctgttttt caaatccata
     1261 agcggaaacg gctcaacaaa tgccgaagga caaaagtaat aaataagggg aagttggccg
     1321 ggctgatacc tgcgcggtaa gtgggccaat tggaatttcc cctgtgaaaa atcgtacctt
     1381 attatttttg gtgctcatta tcttttgccg cgaaggcccg gaaaacgctg gtttcattcc
     1441 gaacgtgagc caccggtgcc aagccatgcg catggaaaaa ggacctgaaa ggctccttca
     1501 gccgtttgaa cgcgtaaggg cgacaggaat aaagatggca gcaggaaaat atccggggta
     1561 tctttatcct gcaattcacg gaaaaacatc cggcggtatt ctcaatccat gatcccatga
     1621 aaaatattac ccactccgtt ttcattatcg ccctgctcgg gtcgtttttc ccgaaaaacg
     1681 catccgccca gtgcaccgct ttcttcacgt ggacccagac ggcgaacaac acgatcacat
     1741 ttaccgacgc ttccaccgga aactgcggcg taccgatgcg gcagtgggcc ttcggtgaca
     1801 tgagctccgg tttcggcggc aaccctgtac acttctattc cgctccgggc aattacatcg
     1861 tcacactcat tgtaaccgac tcgatcagca gcagctggca gacacaattc accgatacga
     1921 taacagtgac gggaactgtt ttctgcaatt tgtcttcgtg gatcggcatc agccagcacc
     1981 cgacctgctc cacctgcgcc gacggagcgc tttacgccgg ctcgaacaac ggcactgcgc
     2041 cgttcactta cttatggagt accggggctg tcacaccgat gatcagcaac ctgaacccgg
     2101 gtttttattc cgtctgcgtt accgacgcca acaattgcca ggtgtgcgat acgctcacac
     2161 tcgatacggt gccgtattat tcatcgtgca taccttcgtt cacacacacg caaagccagc
     2221 cgaacgtcgt cgatttctcc ggctcgacga tcctcacgca acccatgtgg ggctggtcgt
     2281 tcggggataa ttccggcggt tacggaccga acacttcgca cacctatgcg tacacgggcg
     2341 tgtacaacgt ttgcctgtcc gtatgggact cgctttcggg atgcagcgga acctattgcg
     2401 acacggtttc cgtgtggggc aacaacgcca gctgccaggg aagcttttat tcgtattacg
     2461 attcgctggt gccgaatacc gcgtgggtcg tcaaccagag tgtcggtgcg cagccgattt
     2521 cctgcacgtg gtactggggc gataacacgt tcgatacgat tcaatatcca tcgcatacgt
     2581 acacggtttc gggaacgtac agcatttgtc tcgtcatcca cgacaacgcc ggctgcacgg
     2641 attcggtttg ccagaacatc gccgtttacc gcatggctta ttcttccgca agcgttcctt
     2701 tctacgtgaa tgtcgttggt cctaccggaa tacgtgaatc gtcgcaacaa tcatggagcc
     2761 tcttccccaa tcctgcggaa aatgagatcc ggatcaaaac agattacagc cttaacggga
     2821 aacaataccg gatcgtcgac attagcggaa ggatcctggt cagcagccgt ctcgacggaa
     2881 atcgtatcga catcagttca ttcgacaaag gaatttattt cctgcagatc gaaaacggag
     2941 ataacggagc attctcttcg cagcgcttca ttaagaatta ggcaaacata tccatacata
     3001 aaaaaaccgg ccgggagatc agttcccggc cggttctttt atgacgcctg ctttttatca
     3061 gttcagcaat ccgtcgagga tgtttttctg ccagtctttc catgtctgcc cgatggcgta
     3121 atcacggatg gtcttgcggt ttttatcctg gttataaaaa tacagcagca cttcgtcgca
     3181 gggattcgcc aggcgcgtgt ttgcgctgaa tgaagcgtcg acgagatttt tcatcagcga
     3241 ttcgtcgaaa tagtcgagcc gtttgagcgc ctgcgcggca ttcacgcgcg tgcggaattc
     3301 gtacgacatg ttcgtaagcg caacaaggcg atcgatggac gctttgtcgc ccgtggtcct
     3361 cgcattgatc tcgagccatt tcacttccac gttgcggccg gaagtcccga tgacgccttt
     3421 cgtcatgtcg aggtatttcg cggctttatc cgggaacagc ccggaaagtt tatcgagcgt
     3481 gctcatcacc acttcatacg agggcgagct cagcagcttt tcgtaatcgc cttccatatc
     3541 ggcggcgacc atagttgtgt tgttgatgac attcctatgc atcagcacgt ctttgtcatt
     3601 gatggcggaa cgaatgatgc tgcggctgtc gggattcgtg tcgttgatca attgcgcgac
     3661 gatctcgcct ttcgttgcct ggaacgtttc tttcgcgtac acctgtttca gcacttcgcg
     3721 tttctgctcc agcgggtaat cgcgcagggc gaccaatgca tcgtaacggt cgagcatgtt
     3781 cggtgcgttc agggcctggt tcttcagcca gtcgaatgat tttttgaacg aaaccgtttt
     3841 cagcacttcg ttgttcggat cgaacagcac gtaagaaatt ttcttgccgg aaggattggg
     3901 aatgtcaacg atctccgttt ccttttcgat ccactgctgc acgcgatcgc tcgtgccgtc
     3961 ggcgtaatgc acttcgaacc agatcggcat tctgaaaagt ccgaccacat cgcttcgctg
     4021 ctgcacctgc ttcacgttga aacgcgtgaa atgcctgccg gaaatattat ccgtaaggtc
     4081 gttcgcatcc acatcgtagg aaggctcgcc gccgtggtac acccattctt cccagaacca
     4141 gttgagcgaa aggccgagcg attcgttgaa gccgttgagc agatcatcgg acgtaacgtt
     4201 gctgtacgca tgatgatcga ggtaatactt gatgccgcgg ttgtatgctt cgcggctgcc
     4261 gacgacatat ttcagcatgt tcagcacgaa tgcgcctttc gggtaatgac gtgcgctccc
     4321 ggcctggctg tgcgcgatcg gaaggtcatt tttctttcct tcatcgagcg cggcgttttc
     4381 agcttcgcgg cgctgccagt tgaaatgatc gtcgccccag atgctgcgtt cgaagagctg
     4441 gtcgtaatac gtggcgaagc tttcctgcag ccagtgatgt gcatcgcttt tcgccgtaac
     4501 gtaatcgccg aaccactggt gcgcgagctc gtgcgcattc acgcccacgt aaccgcggtc
     4561 ccagttgctg cggtcgtcga cgaaaagaaa atcgccgtac accgttgccg tcacattttc
     4621 catcgcgccg tacatgaatt cctgcacggg gatctgcgaa taggaaggcc agggatattt
     4681 cacgccgatc tccgattcat aaaaatcgat catctgctcg ctgtactggt acgtcacgtc
     4741 gacgcggtct ttccagtcgg gataataata gagatgcatg ggcactcccg atttcgattt
     4801 cgtttccttg atgtcgtaaa tgccgatgcc gagcatgacg agatacggcg cgtacggttt
     4861 cgacaggcgg taatgccagc gtttcgttcc gtccttgttg tctttctctt caaccttatc
     4921 gccgttcgac aacactttgt actggctgtc gaatgtgacg aacatgtcgg tcgtgatctt
     4981 gtcgttcatc tcgtcgtaga acgggatcca gttgcggttg tcgatgccct gtccctggct
     5041 ccacacctgc ttgcggcaga tgccggcggg atcgttccag cccacgaaat acaacccgtg
     5101 gcgcgggttc gcttcgtaca cgagcgtcat gctgtcggtc tcgttccacg tcagcgtttt
     5161 cggcattttt atccacgtgc cctggtcgag cgtgcggtag gtcacctgtt ttccatttac
     5221 agatgcttca aggatgcgta ttcccgggcc gtccacccag atggaatcga ccgctttctg
     5281 cagcggggtg aaacggtgga tgacttttcc tttgacgagt cccgcgtgga catcgaacga
     5341 aacatccaat gtcatgtgaa tgaaatccac gttatgctcg cgcggaaccc aggcgccgtc
     5401 gtcgagatac gtgcgtttcg cgccggtgaa agaaccgttc ccgacgaatt gagcgccggc
     5461 cgcaacggca aagaacaatg cggcggcagt aagtaatttt ttcatactga aattccggtt
     5521 ggtgaaagag ttccaatttt aaggaattat ctgttttaaa attcatttta tagctgaagg
     5581 gcgaaacgaa ccgggtgaaa cgaggcaatt gaaggatggt ttgcggcagc aaggaacttt
     5641 gggtcgggaa acgaaggtga aaatcctttg cgctaccttt gcccgctcta tgggtaattt
     5701 ttccattctc attatcgatg atgaaccgaa gctgcgcagc ctgctggcgc ggatgctgga
     5761 actggaaggt tatccagttt cacaggcgga aaatgcgggg aaagggtggg aatacctgaa
     5821 aacacatccg catacgtccc tggtgctgct cgacgtccgt cttcccgatg aaaacggaat
     5881 ttcctttctt gaaaaactga aagccgggta tcccggaacc gaagtgatcg tgatgacggc
     5941 ctacggaacg atccgcgacg ggatcaaagc catgcagctc ggcgcgttcg attatcttgt
     6001 aaagggcgac gacaacgaca agctcatccc ttccgtgagc cgggtaaagg aaaaagtgtt
     6061 gctgcagcgc cgcatcgaga accttgaaaa caggatcggg gaaaaattcc gcttcgagaa
     6121 catactcggc acgtcgatgg tcatccggga agcggttcgc ctcgccgaaa aggtcgcgcc
     6181 caccgattcc accgttcttc tcgaagggga aaccggcgtg ggtaaagaac tttttgcaca
     6241 gtcgatccat tctgcgagcc cgcgaagcat gcagtctttt gttgccgtca actgcagcgc
     6301 gttcccgaaa gatctgctcg aaagcgaatt attcgggcat aagaagggcg cttttaccgg
     6361 cgctgcctat gataagaaag gcctgttcca ggaagccgac cagggcacgc tctttctcga
     6421 cgagatcggg gaaatgagcc tggaattgca ggcgaaattg ctccgcgtcc tggaaacgca
     6481 gacattcatg cgcgtgggtg atacgaaacc ggtgaaagtg aatgtgcgga tcattgccgc
     6541 aacaaaccgg aacctgaaaa atgaaggcga agccggcagc ttcagggctg atctttattt
     6601 ccggctatcc tctttcgtga tctcagtgcc ggcgctgaaa aaccggccgg aagatattga
     6661 acctatagcg cggcacttcc tgaatcatta tgcttcgcgt gtcgggaaga gagtcaaagg
     6721 aatggatgct gaatttctcg gcaggctgaa aaagcacgac tggaaaggaa acatccggga
     6781 actgaaaaac attatcgagc gcgccgtaat cctctgccag gatgaaatgc tgacgacgga
     6841 attgctgccg gaacagttta cgaatgcgga ggatgcggat gatttcacac tcgctacctg
     6901 cgagaaaaaa cacatcctgc aggtgctgcg ccggaccaac ggcaacaaaa ccaaagcggc
     6961 ggaaatgctc gggattggtt tgacgacact ttaccggaaa attgaagagt acaagcttta
     7021 ttaaccgtgc ccggcctgac atccggaagc tttttccctg gattcagaag aatacaactg
     7081 ccggttgccg ggaaacaacc ataagtagcg gcattaccac taagaggagc ggcactttta
     7141 ttgacacccg gaacgagccc atctaccttt gtaaggtcaa aaaaataaaa aaaaaaaaac
     7201 gatggaagct attgtaattg gcgacaaaat cgccagagca cgaaaaaaaa cgaacctctc
     7261 ccaggcgcaa ttagccgaaa agctgtttat cagtccgcag gcggtgggaa aatgggaacg
     7321 cggagaatct gttccggaca ttgttacaat cggcaagctg gcgaaaatcc tgggcgttga
     7381 cctgaattac ttttcagaca gctctcaatc gatcgaggca gaaacgggcc atgcaggcgc
     7441 tgatgacacc acacaaacag taaacggcag cgatagtcct tcagcagaaa gacggttgct
     7501 gacgaacttc aacggaagcg cgctggcgga gacggacttc acgggcgtaa gcgcgcaaaa
     7561 aagaaaattc atcggaagtg atttgcacgg ttcggatttt tcaggcgccg atctgacggg
     7621 cagctcattt acgggcagcg atctgcatga tgctgatttc gggaggacga acctgacgga
     7681 ttgctccttc gtcgcgcttg atcttacaaa tgcaggattc aacgaaacga ttcttgtccg
     7741 caccgaattc agcagttccg atttgagcgg ggcaacattc cgtaatacaa aactggtaga
     7801 cgtaaagctg actacgaccg acctgaaaaa aacgattttt gaaaactgca cgttcagcgg
     7861 cgtggatttc aaatcttccg acctgagcgg attatgcctc gacggacaga cttttacagg
     7921 cgtcaggttc gacaacgcgg acctgaaaaa tgcttctttt aagggcgcga cgcttaaaaa
     7981 cgtttctttc cgtccatcat ttgcgctgac caacaggtat tacaagacgc tcaaaacaat
     8041 ctcctttgac ggggcaaaaa tggacaaact gacctacgct gcgctcaaag gcgtcggggt
     8101 cgatctgtca aaggtcacaa cgatctgaag ccaccccatt ttgaaaaagc acccttccaa
     8161 aacggaaggg ttttttattt accggcaccg ccgttggtta cagaaatgat tcgctgacag
     8221 gtgattgcct gtccgccaca gcaagggaac agattttggc aatggcttcc tgtcgaccat
     8281 aaaaccaatg gcaatggaca agaagcagat ttcagaatta attctccgga actattacca
     8341 taccggtgga aggactgtac ggttaacgct ggcagacggg tcggttcacg acggaatgtt
     8401 cgcgggttac taccacgacg acatcgacga cggtgaaaat cctccagacc gctggcactt
     8461 cgtaacggaa aaagagttca cggatttttc aggcgctgcg ggcaacaggg aaaatttcgg
     8521 aataatcatc cggaccgaag aaattgtttc cgttcaattc aaaacctgaa gaacatgaaa
     8581 aacacgatag agatacttct gcttgcaggc agttacctgg ttcctttcgt cctcgctatc
     8641 gcatcccgcc tgccgaaaaa aaacaggaat tgacccttgt cagattcctc attgatcaga
     8701 aacatatcat gacactcatt caacgacaac ctttaccaaa aatctgaaac catgctcacc
     8761 gctcttcttt ttctaagcgc cggattttgt ttctggctgt tttacaaatc ggtcgatttt
     8821 ttcgacaaca tctaaaaaac caaagcgcta tgatgacagc cctttttatt atcggactcg
     8881 gcgtattcgt ctatctcgtt tacgtcctta tcaaacctga aaaattctaa caatcattac
     8941 tatgaatacc gaaatcatcg gaataatttt catgtacgca gtgacgcttc tgctcgcgat
     9001 ccctttgggg aaatatatcg gcaaagtgtt cagtggggaa aaagtctgga cggatttcat
     9061 gaatcctgct gagcgattca tcttccgcat ttcggggatc aatccttcgc aggaaatgaa
     9121 ctggaaacag cacctgaaag caatgctcac catcaatttt gtatggttcc tgctttccat
     9181 gcttgtgctg atgaacatgt cgtggctccc gctcaatccc gatcataatc cctcgatgag
     9241 cgccgacctg gcgttcaaca cttccgtgag ttttgtaacg aatacaaatc ttcagcacta
     9301 ttccggcgaa acaggccttt cctatctcgg ccagctgacg ctcatgttgt ggcaattcat
     9361 cagcgctgca accggaacag cgatatgtgc ggtagtgttt tttgcgctgc gtgaaaagca
     9421 gaatccgggg ctcggaaatt tttacaacct gctcgtgaaa tccacaacgc gcatatttct
     9481 cccgatcgcg ttcgttgtcg ccatcatgct tcttttcagc ggaacaccaa tgacgctgct
     9541 gggaaaagat ccggttgtaa cgctgcaggg agattccacg caggtcagcc gcggacccgt
     9601 tgctgcaatg gttgcgataa aacagattgg tacaaacggc ggaggatact acgggccaaa
     9661 ctctgcgcat ccgctggaaa atccaactta cttctccaac attgtcgaga acatcagcat
     9721 catacttatt ccgattgcga tggtctttgc tttcggatac atcatgcgaa gaaaaaaact
     9781 tgcgcttacg atcttcgctg taatgacagc gggtttcctc tgctttctta ttccttcggt
     9841 gatcgtggaa atgcacggca atcacgcgat ggatcacatg cacgttttac aaagtacggg
     9901 aagcatggaa ggaaaagaag tgcgattagg tccggctgct tcggcgtatt gggcaatcaa
     9961 tactactgta acctccaatg gttcggtgaa tgccatgcac gacagcatga ccccggtaac
    10021 gggcatgttc acgatgctgg gcatgatggt caacagtttc tacggtggcg ttggcgttgg
    10081 attcctgaat tatttcatct tcatcattct tgcggttttc atcagcgggt tgatggtggg
    10141 tagaactccg gaattgctcg gtaaaaaaat cgaagcgaag gaaatgaaga tcgcgatgat
    10201 cattgcattg cttcatccgc tgctcattct taccggaaca gcgatttcaa gccacctgta
    10261 cgccaatgac ccggctaaat attcggggtg gctcaacaat ccgggatacc acggtttcag
    10321 tgaaatgctt tatgaattca cttcatcttc cgcgaacaac ggcagtggtt ttgaaggcct
    10381 gggtgataac acaccgttct ggaacatcgc ctgcggactt gtcatgttgc tggcgcgatt
    10441 cctgcctatc atcgggccgg ttgcgattgc cggaatgctg gcgaagaaaa aattcatacc
    10501 acaatctgcc ggcacgctgc acaccgatac ggcaacattc ggactgatga cattcgctgt
    10561 catcatgatc gtagctgcgc ttgcatttct gccggcactg acacttggtc cgctcgccga
    10621 atatttctca ttgcatcact aataagtaaa cagcattaaa aaataaagtt atgagttcag
    10681 tcagaaaaca ggcaaaggcc atgaagcttt tcgaaaggaa gctggtgctg caagcgctga
    10741 aacaatcgtt cgtgaaactt catccgaaat tcatggtgaa gaatcctgtc atgtttaccg
    10801 tagagatcgg aactgcgatc atgcttgcga tctgcatctg ggtgatgaca ggagaaaaat
    10861 cgcagggcag ccccacatac aactggctga tcttttcgat acttctcctt actgtacttt
    10921 tcgcaaattt tgccgaagca attgcagagg cgcgcggaaa agcacaggca gaatcgctgc
    10981 gcaaaacgcg tgaagaaacg cctgcgaaac tcattgttcc gcttggcgaa ctgtatgtga
    11041 atgaagtgca gaaagtaagt tcttcgcaac tgaaaaaagg cgatgtcttc ctgtgcgagg
    11101 caggcgatat catcccgctc gacggcgaga tcatcgaagg catcgcgacg atcgacgaat
    11161 cggcgattac cggcgaatca gcgcccgtga tccgcgaagc gggcggagat aaatcgagcg
    11221 tgaccggcgg cacgaaagtg ttgtcagacc aggtcaaggt aaaagtcacc gttgaacccg
    11281 gcgaatcctt cctggataaa atgatcgccc tcgtggaagg cgcttcgagg cagaaaacgc
    11341 cgaatgaaat cgcgctgacc atcctgctcg ctgcattcac gctcgtcttc attattgtgt
    11401 gtgtaacgct taaaccattt gcggattacg cacaaacgcc gatcaccatc gcggcattca
    11461 tttcactttt tgtttgcctg atcccgacaa caattggcgg attgctttct gcgatcggaa
    11521 ttgcgggcat ggaccgtgcg ctgcaggcaa acgttatcac gaaatcaggc aaagcggtgg
    11581 aaaccgcagg tgatgtggat gtgctgctgc ttgacaaaac gggaacaatc accattggaa
    11641 accggaaagc gacgagtttt cacccggctc cggggatcag cggggaaaaa tttatgggcg
    11701 actgcgtcct ggcgtcgctg gccgacgaaa cgccggaggg aaaatcgatc atcgagctgg
    11761 cgaaaagcct cggcgcaaaa acacatgcag atgaatcgct gatgagagac gcgaagttta
    11821 tccagtttac ggctgaaacg agaagcagcg gcatcgagct gaagaacggt atcgtcgtcc
    11881 gcaaaggcgc gtcagacgcg atcgcaaatt tcgcgaagaa agcagggaat gcatttccgg
    11941 atgaaacgca gaaaaaagtc agtgagatcg cttcgaacgg ggggacaccg ctggtagtcg
    12001 ccgaaaacaa taaagtgctc ggcgtgatcg aactgcagga catcatcaaa cccggcatcc
    12061 gcgagcgctt cgaacgcctc cgcaaaatgg gcgtgaagac ggtgatggtg acaggagaca
    12121 atccgctcac tgcaaaattt atcgctgaga aagcaggtgt agatgatttt attgccgaag
    12181 caaagccgga agataaaatg aactacatca agaaggaaca ggcgaaagga aaacttgttg
    12241 cgatgatggg cgacggaacc aatgatgcgc ctgcgctcgc acaggccgac gttggcgttg
    12301 ccatgaacag cggaacgcag gcggcgaagg aagccggcaa catggtggac ctggataacg
    12361 atccgaccaa actcattgaa gtggtggaga tcggcaagca gttgctcatc acgcgcggaa
    12421 cgctgacgac attttccatt gccaacgacg tggcaaaata ttttgcgatc gttcctgcct
    12481 tgttcatcgc ttctattcct gcattgcagg gaatgaacat catgaacctg cacagcccgg
    12541 aaagtgcgat cctttcggca gtgatcttca acgcgatcat catcccgatt ttaattccgc
    12601 tcgcgctgaa aggagtgaaa tacaaaccga tcggcgcaag cgcattgctg cggagaaatc
    12661 ttctcatcta cgggctcggc ggagtgatta ttccattcgt cggaataaaa ctcatcgacc
    12721 tgttcgttgg aatattcttt taattaattg aaaaaaataa aaaaatgaaa ccgtatttat
    12781 taccatcatt aaaactcacg ctggttctta tcgtgatctg ttcgtgtttg tacccgcttc
    12841 tggtagcggg aattgcaaag gcagctcccg gcggcggagg gggtgtaacg gtgagcgtga
    12901 acggaaaagt agtgggctac gaaaacatcg ggcagaagtt tgaccgtgac gattatttct
    12961 ggagccgtcc ttctgcagtc gattacaatg ctgctggttc cggcggatcc aacaaaggcc
    13021 cgacgaatcc tgattatctc gcacaggtgc aggcgcgcat cgacacgttc atggtgcata
    13081 atcctgatgt gaataaaaat gaaatcccgg ttgatcttgt tactgcatca ggcagcggac
    13141 tcgacccgga tatttctatc caggccgcaa aatgccaggt cccgcgcatt gcaaaaatca
    13201 ggaatatgag tgagcaggat ttgtatgcac tcattgataa aacaaaacaa ggcccactgc
    13261 tcggatttct cgggcctgaa aaagtgaatg tgctcaagct gaatattgcg cttgatgcga
    13321 aataaataaa tgaatgaatt gattcaatat ttatagcaat gatttca
//
LOCUS       DASTSF010000574         2399 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1023045,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000574 DASTSF010000000
VERSION     DASTSF010000574.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1023045"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..378
                     /locus_tag="VFU15_16255"
     CDS             <1..378
                     /locus_tag="VFU15_16255"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEU4719399.1"
                     /translation="GATLQYYNATQAGNYSVEAFTYQGCSSGMSPAVLVDGIEENGDL
                     SFGIYPNPNSGEFDLSFTTPGISDYRLDIFSVDGKTVYSEDLQQFSGTYSRHIDIRSA
                     GSGSYVIRLTDGGKETIRRIIVF"
     gene            381..917
                     /locus_tag="VFU15_16260"
     CDS             381..917
                     /locus_tag="VFU15_16260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEU4719400.1"
                     /translation="MSLVSQWKIDKSWTLFLDRDGVINVRFPGDYVKHVGEFGFLPGA
                     KEAIARLSGIFGKIIVVTNQQGIGKGIYTEEDLASIHAHMVSEIEKAGGRIDAVYFAP
                     HLESENSPMRKPGTGMAGQAKKDFPSIDFSRSVMIGDSASDMVFARNAGMKAVFIGTG
                     ENLPGNPDFTCASLSDFR"
     gene            1102..1479
                     /locus_tag="VFU15_16265"
     CDS             1102..1479
                     /locus_tag="VFU15_16265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009285465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEU4719401.1"
                     /translation="MNGSAIDYLPIVFTLIVAGGFVGTTLVLTHILGPRRKTSKKLET
                     FECGIEIQGNARIPFSVKYFLVAILFVLFDVEVIFMYPWAVNFRGPQGLGITGLLEMI
                     SFVLLFVIGFYYILSKGALKWED"
     gene            1501..2064
                     /locus_tag="VFU15_16270"
     CDS             1501..2064
                     /locus_tag="VFU15_16270"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010601906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HEU4719402.1"
                     /translation="MNATDNARTKLEIAKSPLGMEGPGFFATSLEKAVGLARKNSIWP
                     LPFATSCCGIEFMATMASTYDLGRFGAERLSFSPRQADLLMVMGTIAKKMGPVLKHVY
                     EQMAEPRWVLAMGACASSGGIFDTYSVLQGIDRIIPVDVYIPGCPPRPEQVLDGILQI
                     QKLVENESLRRRDSDEYKAMLNKYGIE"
     gene            2076..2288
                     /locus_tag="VFU15_16275"
     CDS             2076..2288
                     /locus_tag="VFU15_16275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002697082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719403.1"
                     /translation="MSRNWEYATIEWLWDSGNFRVNLPGNRERNSPGSYSELVALLNE
                     MGGEGWDVASCTSGGNWLFWTLKREK"
BASE COUNT          577 a          640 c          627 g          555 t
ORIGIN      
        1 ggcgcaacgc tgcagtatta caatgcgacg caggcgggaa attattcggt cgaggcattt
       61 acttaccagg gatgctcttc gggaatgtcc ccggcagtgc tggtggacgg gatcgaggag
      121 aacggagatc tttccttcgg aatttatccc aacccgaaca gtggtgaatt cgacctgagc
      181 tttacgacac ccggcatttc cgattaccgc ctcgatattt tcagcgtcga cggaaaaact
      241 gtttattccg aagatctgca gcagttttcc ggaacataca gccggcacat cgatatccgt
      301 tccgcgggct ccggctctta tgtcattcgt ctcaccgacg gcggaaagga aacgatccgc
      361 cgcatcatcg tgttctgacc gtgtcgctcg tttctcaatg gaagatcgat aaaagctgga
      421 cgctgttcct cgaccgggac ggcgtgatca acgtgcggtt tcccggcgat tatgtaaagc
      481 acgtcggcga gttcgggttt cttcccggcg cgaaagaggc gatcgcaagg ctttcgggga
      541 ttttcgggaa gatcatcgtg gtgaccaacc agcaggggat cggcaaggga atttacacgg
      601 aagaagatct cgcctccatt catgcgcaca tggtttcgga aatagaaaaa gccggcggaa
      661 ggatcgatgc ggtatatttt gcgccgcacc tggaatcgga aaattcaccg atgcgtaaac
      721 cgggaacggg aatggccggg caggcgaaaa aagattttcc ctcgatcgat ttttcacggt
      781 cggtcatgat cggtgattct gcgagcgata tggtgttcgc gcggaacgcc gggatgaaag
      841 cggtttttat cggaacaggc gaaaatcttc cgggtaaccc ggatttcacc tgcgcatccc
      901 tttccgattt ccggtaagtt ttcgtcccga ttcatcactt catcttcaga atctgtacac
      961 ccggcgatta atacccaaaa cgcggtattt cattcagata tttccctatt tttaaccccg
     1021 ttcttcgacg cccttccagg tacggaaaac agatgatttt cgcctttcag aatacagaga
     1081 aagcaaacca aaaacagagt catgaacgga agcgcaattg attatctccc tatcgttttc
     1141 acactcatcg ttgccggtgg attcgtcggc acaacactcg tgctgacaca cattctcggc
     1201 cccagaagaa agacctccaa aaagctcgaa acgttcgaat gcggtatcga gatccagggc
     1261 aatgcgcgca ttcctttttc cgtaaaatat tttctcgtcg ccattctttt cgtgcttttc
     1321 gatgtggaag tgatcttcat gtacccgtgg gccgtgaatt tccgcgggcc gcagggactt
     1381 ggcatcaccg gtctcctgga aatgatctcc ttcgtgcttc tttttgtgat cggattctat
     1441 tacatcctca gcaaaggcgc cctgaagtgg gaagattaat cagcaataat ccgtctcgtc
     1501 atgaacgcaa ctgataacgc aaggacaaag ctcgagatcg caaaatctcc gcttgggatg
     1561 gaaggtcccg ggttctttgc tacttcgctt gaaaaagccg ttggccttgc ccgcaagaat
     1621 tccatctggc cccttccttt cgcaacctcc tgctgcggga tcgaattcat ggccacgatg
     1681 gcctccacat acgacctcgg ccgtttcggc gcagaacgac tcagtttctc gccgcgccag
     1741 gccgacctgc tgatggtgat gggaacgatc gcgaagaaaa tggggccggt gctcaagcac
     1801 gtgtatgagc agatggcaga gccgcgctgg gtgcttgcca tgggcgcatg cgcttcgagc
     1861 ggcggcattt tcgataccta cagcgtactg cagggaatcg accgcatcat ccccgtggac
     1921 gtatacattc ccggctgtcc cccgcgcccg gaacaggtgc tcgacggcat cctgcagatc
     1981 cagaagctgg ttgagaacga atcgctccgc cgcagggatt ccgacgaata caaagccatg
     2041 ctcaacaaat acggaatcga ataatttttg cgcggatgag caggaactgg gaatacgcaa
     2101 cgatcgaatg gctgtgggac agcggaaatt tccgcgtcaa ccttccgggc aaccgggaac
     2161 gcaattctcc cggcagctac agcgagcttg ttgcgcttct caatgaaatg ggcggtgaag
     2221 gatgggacgt ggcgtcctgc acttccggcg gcaactggct tttctggaca ttaaaacgag
     2281 agaaataaaa caccaatctc cgaacaaaag actcccgact aaaaatggct gtggcaactg
     2341 cagaacaagt tcaatccctt cacgacaccg ttctttcgcg cctgaaagaa aaattcggc
//
LOCUS       DASTSF010000575         3431 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1566160,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000575 DASTSF010000000
VERSION     DASTSF010000575.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3431
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1566160"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..504)
                     /locus_tag="VFU15_16280"
     CDS             complement(<1..504)
                     /locus_tag="VFU15_16280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003012169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719404.1"
                     /translation="MHTIEPFFNWRHLYTSEEDERSPFFEREYSEFEFTNTIYNYLIH
                     PQWDDFGSSTLYIKILFVDYENSSCVIELIGEWNDALYNDIMILKRDIIEELQRYGIN
                     RFILLGENVLNFHYSDESYYEEWFSEIDDGWIAFVNFRPHVLHEMKRARLDWYVNWGG
                     ELDDLPWR"
     gene            complement(532..1332)
                     /gene="murQ"
                     /locus_tag="VFU15_16285"
     CDS             complement(532..1332)
                     /gene="murQ"
                     /locus_tag="VFU15_16285"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HEU4719405.1"
                     /translation="MEKITESESRYDHLEKMSTEEILTSINREDRTVPEAVGKIIPDI
                     GKLVDVVAEKMKNGGRLFYLGAGTSGRLGIVDASECPPTFGVPHGLVVGIIAGGDSAI
                     RRAVEFAEDDTEQGWKDLQEHVIGKEDVVIGIAASGTTPYVVGALKKCNAEGIITGCI
                     TCNPGSPAAMEAKHPLVAVVGPEFVTGSTRMKSGTAQKLILNMLSTGVMIKLGRVKGN
                     RMVDMQLSNNKLIDRGTKMVMEATGLSYEKANDLLKKHGSVRKAIGNK"
     gene            complement(1419..2492)
                     /locus_tag="VFU15_16290"
     CDS             complement(1419..2492)
                     /locus_tag="VFU15_16290"
                     /inference="COORDINATES: protein motif:HMM:NF039201.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEU4719406.1"
                     /translation="MKKLLLPFFLFSATAVSAQITVTTNDVGPIYNQFYNAVDTVPPN
                     ISPGTPGPSQTWNYSTLTSEYTDTLTFTLPWFTSYGSSFPSSNTSVATRSMGTDSWTY
                     LTNTSSSLQINGEAADPLGTGTMAIPFSDPEKMLDFSSTYGSAYTDTAKAYTQFYYGQ
                     DPGIGQTVDSVRVHIWVKKTSDIDAWGSLTDPTSTYDVLRQNVLRVEYDTIDAYIQLF
                     GGWIPDAIDQMDSNRVYTFWANAVGYPVVQLTDHQDLDSITKTEYLLGSAMVGMGEQM
                     RNEHMYAFPNPANDNITFNTQGAGAATIVIYNSTGQLVQSMNVLSDKTVLNVSGYDAG
                     VYFYQAKDASGNTLSNGKFAVTH"
     gene            2710..>3431
                     /locus_tag="VFU15_16295"
     CDS             2710..>3431
                     /locus_tag="VFU15_16295"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU4719407.1"
                     /translation="MKKVSLNDLAKALNLSKTLVSFVMNGKGDAYGINPETQKKVQAM
                     AKKMNYRPNLIARGLRTGKSNLLGLIVSDIGNPFYSRISRAVEAEAEKHGYYMMVCST
                     DENETREKRLVQMLLEEQGVNGLIISTSQHDPEYFRELKKSGYPLVLIDRSINGLKID
                     YVGVDNYKGGFEATMQLLNVGYKKIAMLRISPSFLSTINDRARGYMDALKERGIKPDP
                     KLIREIPFDDIREKVFTAVREL"
BASE COUNT          789 a          870 c          927 g          845 t
ORIGIN      
        1 ccgccaggga agatcgtcca gctctccgcc ccagttcacg taccagtcga ggcgggcccg
       61 cttcatctca tgcagcacgt gcggacggaa attgacgaaa gcgatccacc cgtcatcaat
      121 ttctgaaaac cattcttcat aatagctttc atcggaataa tgaaaattca gcacgttctc
      181 gccgaggaga atgaaacggt tgatgccgta ccgctgcagc tcttcgatga tgtcgcgttt
      241 caggatcatg atgtcgttgt acagcgcatc gttccattca ccgatgagct cgatcacgca
      301 ggagctgttc tcgtaatcca cgaaaagtat tttgatgtac agcgtcgacg atccgaaatc
      361 atcccattgc gggtggatga ggtagttgta gatcgtgttc gtgaattcga attcgctgta
      421 ctcgcgttcg aagaacggtg aacgttcatc ctcttccgaa gtgtagaggt ggcgccagtt
      481 gaaaaaaggt tcgatggtgt gcatcggaat gctcgttgac gggttctcac attatttatt
      541 cccgatggct tttctcacgc tgccgtgttt cttcagcagg tcgttcgctt tttcgtagct
      601 gagcccggtg gcttccatca ccatttttgt tccgcggtcg atcagcttgt tgttgctcag
      661 ctgcatgtcg accatccggt ttcctttcac gcggccgagc tttatcatca cgccggtgga
      721 gagcatgttc aggatcagct tttgcgcagt gccggatttc atgcgcgtgc tgccggtaac
      781 gaattcgggt cccaccacgg cgacgagcgg atgtttggct tccatcgctg ccggtgagcc
      841 gggattacag gtgatgcagc cggtgatgat gccttccgcg ttgcattttt tcagcgcgcc
      901 caccacgtaa ggtgtcgtac ccgaagcggc gatgccgatc accacgtcct ctttcccgat
      961 gacgtgctcc tgcagatctt tccatccctg ttccgtatcg tcttctgcaa attccaccgc
     1021 cctgcgtatc gcgctgtcgc cgcctgcaat gatcccgact acgagcccgt gcgggacgcc
     1081 gaacgtgggc gggcattccg atgcatccac gataccaaga cgtccgctcg tgccggcgcc
     1141 gaggtaaaaa agacgtccgc cgttcttcat cttttccgcg acaacgtcga ccagcttccc
     1201 gatatccggg atgatctttc cgacagcttc gggaaccgtc cggtcttccc ggttgatcga
     1261 cgtaaggatc tcttccgtgc tcatcttttc caggtgatcg taacgcgatt ccgattccgt
     1321 gattttctcc acttttttat tttaaagata atgccgaact gcccgaaaaa agaaacggcc
     1381 ggaaattttc cggccgtaag atagcttcag ttgaaaaatc aatgtgtaac ggcgaacttc
     1441 ccgttggaaa gcgtattgcc cgaagcatct ttcgcctggt agaaataaac gcccgcatca
     1501 tatcctgaaa cgttcagcac cgttttgtcg ctgagcacgt tcatggattg aaccagctgc
     1561 ccggttgagt tatagatgac gatcgtcgcc gcacccgcgc cctgggtatt gaatgtgatg
     1621 ttgtcgttcg ccgggttggg gaatgcatac atgtgttcgt tgcgcatctg ctcacccatt
     1681 cccaccatgg cggaaccgag gaggtattcc gttttggtga tcgaatcgag atcctggtga
     1741 tcggtgagct gaacaaccgg gtagccgacc gcgttagccc agaaagtata aacgcggttg
     1801 gaatccatct ggtcgatcgc atcgggtatc catccgccga aaagctggat gtacgcgtcg
     1861 atcgtgtcgt attcgacacg cagtacattc tggcgaagca catcgtacgt cgaagtggga
     1921 tcggtaagcg atccccatgc atcgatgtca gaagtttttt tcacccagat atgaacgcgc
     1981 acggaatcaa cagtctgccc gatgccggga tcctggccat agtaaaactg ggtgtacgct
     2041 tttgcagtgt ccgtgtaggc ggagccatag gtggaagaaa agtccagcat cttttccgga
     2101 tcggaaaaag ggatcgccat ggtgccggtt cccagcggat cggccgcttc gccgttgatc
     2161 tgcagcgacg agctcgtgtt cgtgaggtac gtccagctgt cggttcccat gctgcgcgta
     2221 gccacggaag tgttcgagga cgggaaagaa gaaccgtagg aagtaaacca cggcagtgtg
     2281 aacgtcagcg tgtccgtgta ttcgctcgtc agcgtgctgt agttccaggt ctgcgaagga
     2341 ccgggcgtgc ccggggaaat attcggggga accgtgtcga cggcattgta gaactggttg
     2401 tatatcgggc cgacatcatt ggtggtgacc gtgatctgcg cggagacggc agtggcggag
     2461 aaaaggaaaa agggaagtag cagtttcttc atgagaattg gttttggatg acaaatttat
     2521 acaatataga aaaaaacgtc cgggaacagg ggggacggtc tttcattaaa ataagatgaa
     2581 cggaatagtt gctgcagaat aaatgatttc atcgccggcg aaatcattca cctttattgc
     2641 cgataatttt atatttttca ccttatttat tctgaacgat ttagatgtac atactttaaa
     2701 aactgcagaa tgaagaaggt ttcactgaac gatcttgcca aagcccttaa cctttcgaaa
     2761 acgcttgttt catttgtgat gaacggcaaa ggagatgcct acgggatcaa cccggagacg
     2821 cagaagaaag tgcaggccat ggcgaagaag atgaattacc gcccgaacct tattgcccgc
     2881 ggattgcgta ccggtaaatc caacctgctc ggtctcatcg tttccgatat cggtaacccg
     2941 ttctattcgc gcatatcgag agcggtggag gcggaggcgg aaaagcacgg ctattacatg
     3001 atggtgtgca gcaccgatga aaatgaaacg cgtgagaaaa ggctggtgca gatgctgctc
     3061 gaagagcagg gcgtgaacgg acttattatt tcaacgtcgc agcatgaccc ggaatatttc
     3121 cgcgagctga aaaaatcggg ttacccgctc gtgctcatcg atcgttccat taatggactg
     3181 aagatcgact acgtgggtgt ggataattac aagggcggat tcgaagcgac gatgcagctg
     3241 ctgaacgtcg gctacaaaaa gatcgcgatg ctgcgtattt ctccttcgtt cctgagtacg
     3301 atcaacgacc gtgcccgcgg ctacatggat gcgctgaagg agcgcggcat caaacccgac
     3361 ccgaagctca tccgtgaaat tccttttgac gacatccgcg aaaaagtctt taccgccgtg
     3421 cgcgagctta t
//
LOCUS       DASTSF010000576         3987 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1675901,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000576 DASTSF010000000
VERSION     DASTSF010000576.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3987
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1675901"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1376)
                     /locus_tag="VFU15_16300"
     CDS             complement(<1..1376)
                     /locus_tag="VFU15_16300"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEU4719408.1"
                     /translation="MKTTCYFIISFFSFLAANLQAQTYTQTTVVTGMSYPVAFAIAPD
                     GRFFCTQKGGNSDPAVNAQIKVYNSTGTYLGVFYDLTDSVDADFERGLLGIELDPNFT
                     TNHWLYVYYVHLWNSDERIRIVRFTENNNVGTNPRIIFDFNVPDNLPGNHFGGNLHFN
                     HTDTTHLYFTIGDFAINQTVPAQNYGQDTTVPFGKTNRIFKYPVNYNPASNWSANIPT
                     DNPFYDDGDPTTGACDLIWSYGHRNPFDFCFSPITDTMYVSENGLNTWDEVNVIHRGG
                     NYGWAYCEGDYLNSSTTSPCNNPAYINPITEWGAPLGGITGIVYYTGPVMPEFDDHLL
                     VADNDYGRVFDITLGNSPAFDMATSNVLWMDMTTSGGLTTLREGSDGCIYAMKGGYTT
                     NGQIYRVCPQGLGTEDFENPVFGLQQNAPNPFSESTVIKYNMKQAAQAKIVIYDIFGR
                     EVAVLADG"
     gene            1464..1970
                     /gene="folK"
                     /locus_tag="VFU15_16305"
     CDS             1464..1970
                     /gene="folK"
                     /locus_tag="VFU15_16305"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017550036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEU4719409.1"
                     /translation="MNTTKSGVFLSLGSNVGEPALNIGEAIRRIAEIPSVKVRRKASL
                     YLTKPWGKADQPDFINTVIEIETELGPQELMEELLRTEKECGRVRMEKWGPRIIDIDL
                     VLFRDVVVNDEKVKVPHPEMHLRKFVLVPLAEIAPDAVHPVYGKTIAGLVKACADRLD
                     VNIVSYCV"
     gene            1974..2579
                     /locus_tag="VFU15_16310"
     CDS             1974..2579
                     /locus_tag="VFU15_16310"
                     /inference="COORDINATES: protein motif:HMM:NF013843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEU4719410.1"
                     /translation="MDYRYIAIEGNIGAGKSTLAKMLAGRLGGTFVPEKFGDNPFLVP
                     FYEDPARHALPAELSFLEDRFRQVRDLEDETGVIVSDYAWEKSIVFARINLKGEELRL
                     FERIYNLLSGQLRPPDILFYLHRPVEKLVRNISSRGRDFEKNIQADYLGKIAAKYRDY
                     LSGEKTIPVIWMSEDLGPEALTADVLKKLGKAWKPGLHLDP"
     gene            2696..>3987
                     /locus_tag="VFU15_16315"
     CDS             2696..>3987
                     /locus_tag="VFU15_16315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719411.1"
                     /translation="MKKIHLPVLGLAVFAVMSLASCSGLGKMVKKESAVKYTVTPNPL
                     EMHGDSVAVTITVNYPPKYFAKKCVLTVTPQCGDHKFKSVTLLGEKAQGTGTRIAYKT
                     GGSYTYTDKVAYTSDMLDADLKVNATGQVKNKTKDIPGRVVGHGTKITPRLMKNDDKP
                     IIGKDAFVKTVPVTAVGETHFVISSTTYIPKKDAKGDDMKPVNDFMAAHRTDPNMEIK
                     NIGISSYASPDGETDMNAKLADNRFDKTKKDLQTIYKDKKTGYDPATKDDFYQKQVTA
                     EDWDGFQDLVSKSNIADKQIILSVLSKYPDGEKREQEIKNMSATYVELAKDILPQLRR
                     SVVTVNAIKHNRTDDQILKLARTTPDSLSIEEILYAATLTTDMNEKLNFYKSAERMYP
                     TDWRTSNNVGCILLMQNKLQDAKAEFEKAAKLSANNPI"
BASE COUNT         1013 a         1007 c         1087 g          880 t
ORIGIN      
        1 atgccgtccg ccagcacggc cacttcacgc ccgaaaatat catagatgac gatcttcgcc
       61 tgcgccgcct gcttcatgtt gtatttgatc acggtgcttt ccgagaaagg gttcggtgca
      121 ttctgctgca atccgaaaac aggattttcg aaatcttccg ttcccagccc ctgcggacat
      181 acgcggtaga tctgcccgtt cgtcgtatag ccgcctttca tcgcgtagat gcagccgtcg
      241 cttccttcgc gcagcgtggt aagtccgccg cttgtcgtca tgtccatcca cagcacgttg
      301 cttgtggcca tatcaaaagc cggcgaattt ccgagcgtaa tgtcaaacac gcgtccgtaa
      361 tcattatccg ctacgagcag gtgatcgtcg aattccggca tcaccggccc ggtgtagtaa
      421 acaattcctg ttatgccccc gagcggtgca ccccattctg tgatcgggtt gatgtacgcc
      481 gggttgttgc acggagatgt cgtggagctg ttgaggtaat cgccttcgca atacgcccac
      541 ccgtaatttc ctcccctgtg aatgacgttc acttcatccc acgtgttgag gccgttctcc
      601 gaaacataca tggtgtctgt gatgggactg aagcagaaat cgaacgggtt gcggtgacca
      661 tacgaccaga tgaggtcgca ggcgccggtg gtcggatcgc cgtcgtcgta gaacggatta
      721 tccgtgggaa tattcgcgct ccagttgctg gcagggttgt aattcaccgg gtatttgaaa
      781 atgcggttcg ttttcccgaa cggaacagtt gtatcctgcc cgtaattctg cgctggcacc
      841 gtctggttga tggcgaaatc gccgatggtg aaatagagat gcgtcgtgtc cgtatgattg
      901 aaatgaagat ttcccccgaa gtggttgccg ggaagattgt cgggcacgtt gaaatcgaaa
      961 atgatgcgcg ggttcgtccc cacgttgttg ttttccgtga agcgcacgat gcgtatgcgc
     1021 tcgtcactgt tccagaggtg aacatagtac acataaagcc agtgattggt cgtaaagttc
     1081 ggatcgagct cgatgccgag cagcccgcgt tcgaagtccg cgtcgacgga atccgtgagg
     1141 tcgtagaaaa caccgaggta agtgccggtg ctgttgtaca ctttgatctg cgcattgacg
     1201 gccgggtcgg aattgccgcc tttctgcgtg cagaagaaac gcccgtcggg cgctatcgcg
     1261 aaagcgacag gataggacat gccggtgacc acggtggtct gcgtgtaggt ctgcgcctgc
     1321 aggtttgcgg cgaggaatga aaagaaagaa atgatgaagt aacaggtggt tttcatgagc
     1381 ggtcagggaa tggatttgcg gcaagttagg aaaaggaaac ctatcgtgtt gagcaagccg
     1441 gggactttgg ataaattcgc gggatgaata ccacgaaaag cggcgttttt ctttccctcg
     1501 gctcgaatgt cggggagccg gcgctgaata tcggggaggc gatccgcagg atagcggaaa
     1561 taccttccgt gaaggtcagg cggaaagcat cgctttacct gacaaaaccc tggggaaaag
     1621 cggatcagcc ggattttata aatacggtca tcgaaattga aacggagctc ggtccgcaag
     1681 agctgatgga ggaattattg cgcaccgaaa aggagtgcgg gcgtgtccgc atggaaaaat
     1741 ggggacctcg catcatcgac atcgatcttg tgctcttccg tgatgtggtg gtgaacgatg
     1801 agaaagtaaa agtgccgcac ccggaaatgc acctccggaa attcgtcctc gtgcccctgg
     1861 ccgagatagc gccggacgcc gtgcacccgg tttacggaaa aacaatcgcc gggctggtga
     1921 aagcctgcgc cgacaggctg gatgtgaata tagtttcata ctgtgtgtga gcaatggact
     1981 accggtacat tgccatagaa gggaacatcg gcgcgggaaa atccacgctc gcgaaaatgc
     2041 tggccgggcg gctcggcggg acgttcgttc ccgaaaagtt cggcgacaac cctttccttg
     2101 ttccgttcta tgaagacccc gccaggcatg cattgccggc agagctatct tttctcgagg
     2161 accgtttccg gcaggtacgc gatctggaag atgaaaccgg ggtgatcgta tccgattatg
     2221 cgtgggaaaa atcgatcgtg tttgcgcgga tcaacctgaa aggggaggag ctgcggcttt
     2281 tcgagcggat ttacaacctg ctgagcgggc agctgcgtcc gccggacatc ctgttttacc
     2341 tgcaccggcc ggtggaaaag ctcgtgcgga atatttcttc gaggggaagg gattttgaaa
     2401 agaacataca ggcggactac ctcgggaaga tcgcggcaaa gtaccgcgat tacctttccg
     2461 gcgaaaaaac aattcccgtt atctggatga gtgaagatct cggcccggaa gcccttacgg
     2521 ccgatgtgct gaaaaagctg ggaaaggcat ggaaacccgg gcttcatctc gatccctgaa
     2581 acagcctgag acttagtaaa ttctaacaat gacgatttga aaacattcct ttcgtttttg
     2641 gggaattttt ataattttgc gccgtaccaa acaaaaggaa aatcctgatc tgatcatgaa
     2701 aaaaatccac cttccagttt taggccttgc cgtttttgct gtgatgagcc ttgcttcctg
     2761 cagcggtctc ggcaaaatgg tcaagaaaga atcagcggtc aagtatacgg ttacacccaa
     2821 tccgcttgaa atgcacgggg acagtgttgc tgtaacgatc accgtcaatt atccgccgaa
     2881 gtatttcgcg aaaaagtgcg tgctcaccgt aacgccgcag tgcggagatc acaaattcaa
     2941 atctgtaacg cttctcggcg aaaaagccca gggaaccggt acgcgtatcg cttacaaaac
     3001 gggtggatcc tacacctaca cggataaagt agcgtacact tcagacatgc tggatgccga
     3061 tctgaaagta aatgcaaccg gccaggtcaa gaacaaaacc aaagatatcc cgggacgcgt
     3121 ggttggtcat ggaaccaaga tcacgccgcg cctgatgaaa aacgacgaca agccgatcat
     3181 cggaaaagat gcgttcgtga aaacagttcc cgttacggcc gtgggtgaaa cgcacttcgt
     3241 gatcagctct accacgtata tcccgaaaaa ggacgcgaag ggtgatgata tgaagccggt
     3301 caatgatttc atggccgctc accgcacgga tcccaacatg gagatcaaga acatcggtat
     3361 ttcttcctac gcttctcctg acggtgaaac cgacatgaac gcgaagctcg cagacaaccg
     3421 tttcgacaaa acgaagaagg acctgcagac gatctacaaa gacaagaaaa cgggttatga
     3481 tcctgcaacg aaagacgatt tctaccagaa gcaggttacc gcggaagact gggacggttt
     3541 ccaggacctc gtttcaaaat cgaatatcgc cgacaaacag atcatcctca gcgttctttc
     3601 caaatatcct gacggagaga agcgtgagca ggagatcaag aacatgtctg caacgtatgt
     3661 tgagcttgcg aaagacatcc ttccccagct ccgccgttct gtcgttaccg ttaacgcgat
     3721 caagcacaac cgtaccgacg accagatcct gaagcttgca cgcaccacgc cggacagcct
     3781 ttccattgaa gaaattcttt atgcagctac gctgaccacc gacatgaacg agaagctcaa
     3841 cttctataag tcggctgaaa gaatgtatcc taccgactgg cgcacatcca acaacgttgg
     3901 ctgcatcctc cttatgcaga acaagctcca ggacgcaaaa gcggaattcg aaaaagcagc
     3961 caagctttct gcgaataacc cgatcat
//
LOCUS       DASTSF010000577         6714 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_337371,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000577 DASTSF010000000
VERSION     DASTSF010000577.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 6714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6714
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_337371"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            324..1535
                     /locus_tag="VFU15_16320"
     CDS             324..1535
                     /locus_tag="VFU15_16320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719412.1"
                     /translation="MEILSESHLHIMRRFFALVTFCFTAGILSAHPMEIDFDEMIAKS
                     KSIFIAHFISDNLDHTEYKLAVESVLKGHETQAVVKAGKAYGRVFMEKNTRFIAFINE
                     KNQLEWAGFSDSLETGLISLHGFYDYNSYDVDPDAVSLVQLRQFIHEHKYDGSIDGNL
                     SFMNYGTMQQEKTPVHFHISYTYHSGDKVSTECTQDGLNLVDFTNKPSIYFWGSGLRL
                     TYEENEVRPLEIDGYVDSVNADGKSCRAYFNVWEPEDLDRQAFFDYISHPEYGPPFFE
                     IELCINDSMKYPFFYNVNYGFDDYLQYGKTKLKTENFDAPTRDQKGMLSFGGPMSPVD
                     IEASLDSLPDEPRFSEFHGSGTIYFIRGLRIAPWKGTATITQNGKKTAEGKCTVTLKA
                     THFNKNVNYGK"
     gene            complement(1536..4007)
                     /gene="pheT"
                     /locus_tag="VFU15_16325"
     CDS             complement(1536..4007)
                     /gene="pheT"
                     /locus_tag="VFU15_16325"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002999127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEU4719413.1"
                     /translation="MKISLNWLNDYFPSDKSAEEIAALLTGCGLEVESVETFESIPGG
                     LKGLVVGEVMEKTKHPNADKLSLTKVDVGGTELLSIVCGASNVQAGQKVIVATVGTTV
                     HPVEGEPFEIKKSKIRGELSEGMICAEDEIGLGSSHAGIMVLDPSAKTGTPATEYFGI
                     KTDHVLEIGLTPNRADAASHFGVARDLSAVVLAAELLKDVSAEHVLAQLPPAVTFEDG
                     LPVSPVSVDVKDTEACVRYSGIYLTGVEVKDSPEWLQQRLKAIGVRPVNNVVDVTNYV
                     LHELGQPLHAFDAERISGNKIVVRKANEGEKFVTLDGTERKLSANDLMICDAGKPMCM
                     AGVFGGLDSGVTEKTKTIFLESACFNPVHVRKTSKRHSLKTDSSFRFERGTDPEMTMF
                     ALQRAAYLLEEVAGASVAAEAVDIYPVKVEPKQVAFTYAKCETLIGKRIEHAIVRKIL
                     LALGMEIISEGKDALLLSVPPFKVDVTRDADVVEEVLRVYGYNNIGFPDFLRASLSYS
                     PKPDAEKVNDAIADLLAANGFREMLNNSLTKAAYYDLLEGNNASSNVALLNPLSSDLG
                     IMRRSLLFGGLETIAYNRNRKSEDLRLFEFGKTYLKKQDVPEGKWPYVELQRLALFIT
                     GRKEPESWNTKKNPVDFTDLKKSVRMIADRLGLDNMKFSKTESPEFSEAMALAVRKKE
                     VARFGKVKQDICKAFDIDAAVWFADIDRDAILSLLSAAKPVRFSEIPKFPSVRRDLAL
                     VLDKKITYGEIEDIAWQTEKNLLREVNLFDVYEGDKIDAGKKSCAVSFILQDEKATLT
                     DQQVEKVMEKLVKVYSEKLGAVIRS"
     gene            4357..5004
                     /locus_tag="VFU15_16330"
     CDS             4357..5004
                     /locus_tag="VFU15_16330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719414.1"
                     /translation="MKKLTLYALVMTLLLFSCSMEKRIYRNGWYVDRHSTATTKGNVQ
                     QANDDKTFTAAAPVPTQDTLQPSPDSTSLKKTTVDDSTNSPPSFREFFRSMENKVLPP
                     GDGDGRPMRYSEARRSMKDKGCPPNQMAQTVYFMAWASIAATFLGGAGILLAIITLIL
                     SIFAMQNVYDNPDDCVDENIAIIKAARRIALIYLLAAVVAGLAAFVILLWAISML"
     gene            5148..>6714
                     /locus_tag="VFU15_16335"
     CDS             5148..>6714
                     /locus_tag="VFU15_16335"
                     /inference="COORDINATES: protein motif:HMM:NF022481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HEU4719415.1"
                     /translation="MNSTFRKLNNRAGWVVFILAAIVFCSCIEPTASFWDCSEYIACA
                     NGLETGHPPGAPFFLLIAKFFSLFSFGDKTLVAPLVNIMSATASAFTVLFLFWTITHL
                     ARKIMLRKNEDHTSCKMWLVIAAGAIGALSYAFTDSFWFSAEEGEVYALSSFFTAIVF
                     WAMLKWEDMADDPHSDRWLVFIAYMVGLSVGVHLLNLLTIPAMVFIGYFRKTKRITWW
                     GFSLAGICSVLLLGGVQDMIIPGIVNLAGKTELYFVNDLHLPFNSGTIFYFGTLLLLL
                     IGGIAFSHGKKIRWLNTTLIAFTVLLIGYSSFFVLVIRSQAGTPINEGSPTNAISMLS
                     YLNREQYGDWPILYGQYYNSPLDAEQPYLDGNPVYVKDAAKGKYIVSDARKASVPNYD
                     PKGCTYFPRMYDSGHGEGYSSWADVQGTPTTFSDKGGKTFSENIPTFGENMRFFITYQ
                     CGWMYMRYFMWNFCGRQNDRTGYGNDAEGNMLTCIPFVDNERVGNQDLLPPSQKNNKA
                     HNRYFLIPLLLGIL"
BASE COUNT         1552 a         1859 c         1670 g         1633 t
ORIGIN      
        1 catgagcaac acctttggtt ttggcggaca tatcgcaacg agtattttca aaaagtatac
       61 tccctgatct cctgacaacg ggccgggaaa tgcgtgccgg caggtgcttt ttttaatgcc
      121 gatctcagga gtgtttattc ttcgtcttat actgaaaaaa acctgtcgac ctggccggat
      181 cttatcaccg gcaacctgtt tttccgccgg aaacaggact ggaatatcgc atcagggaca
      241 aattcataat tataaaaatc cctcagaaaa acaggcatgc acatttgcag gccaacacca
      301 ttccccctgt ttcccctaat tttatggaaa tcctttccga atcccatctt cacatcatgc
      361 gccgtttttt tgcactcgtt acattttgtt ttaccgctgg aattctttcc gcgcacccga
      421 tggagatcga tttcgacgag atgatcgcca aaagcaagtc cattttcatt gcgcatttca
      481 tcagcgacaa tctcgatcat acggaataca agctcgccgt ggaaagcgtg ctcaagggac
      541 acgaaacgca ggcggtggtg aaagcgggga aggcatacgg ccgcgttttc atggagaaga
      601 acacgcgctt tatcgctttc atcaatgaaa agaaccagct cgaatgggcg ggattttccg
      661 acagtctcga aaccgggctg atctccctcc acggctttta cgattacaat tcctacgacg
      721 tggatcccga tgcggtttcc ctggtgcagc tcaggcaatt cattcacgag cacaaatacg
      781 atggctcgat cgacggaaat ctttcattca tgaattacgg aacgatgcag caggaaaaaa
      841 cgcccgtgca ttttcacatc agctatacgt atcattccgg cgacaaggtc agcaccgaat
      901 gcacgcagga tggtctgaac ctcgtcgatt tcaccaataa accttcgatc tatttctggg
      961 gaagcggtct ccgcctgacg tacgaggaga acgaggtgcg cccgctggag atcgacgggt
     1021 acgtggattc tgttaatgca gatggaaaaa gctgccgggc gtatttcaac gtatgggaac
     1081 ccgaagatct cgaccggcag gctttcttcg attacatctc gcatccggaa tatggtcccc
     1141 cgttcttcga gatcgagctg tgcatcaatg acagcatgaa atacccgttc ttctataacg
     1201 tgaactacgg ctttgacgat taccttcagt acggaaaaac gaaattgaaa acggaaaact
     1261 tcgacgcgcc gacacgcgat cagaaaggaa tgctgagctt cggcggaccc atgagtcctg
     1321 tcgacattga agcgtcgctc gattcattgc ccgatgaacc gcgcttcagc gaattccacg
     1381 gatcaggaac catttatttc atccggggat tacgcattgc gccgtggaaa ggaacagcga
     1441 cgatcacgca gaacggaaag aaaaccgcgg aaggaaaatg caccgttacc ctgaaggcga
     1501 cgcatttcaa caagaatgta aattatggaa agtgatcagc tgcgtatcac cgcgccgagc
     1561 ttttcggaat acactttcac cagtttctcc atcacttttt cgacctgctg atcggtaagc
     1621 gtggcttttt catcctgcag gataaagctg accgcgcagg attttttgcc tgcatcgatc
     1681 ttgtcgcctt cgtacacatc gaaaagattc acttcgcgca gcaggttctt ctccgtctgc
     1741 caggcgatgt cttcgatctc tccgtaggtg atcttcttgt cgagcaccag cgcaagatcg
     1801 cggcgcacgg aagggaattt cgggatctct gaaaaacgca caggcttcgc cgcggacaga
     1861 agcgaaagga tcgcatcacg atcgatgtcc gcgaaccaga cggctgcgtc gatgtcgaat
     1921 gctttgcaga tatcctgctt cactttcccg aaacgcgcca cttctttttt acgaactgcc
     1981 agggccatcg cttctgaaaa ttcaggagac tccgtcttcg aaaatttcat attgtccagg
     2041 ccgagacggt cggcgatcat ccgcacgctt tttttcaggt cggtaaaatc gacaggattt
     2101 tttttcgtgt tccaactttc gggttctttc cgcccggtga taaacagcgc aaggcgctgc
     2161 agctcgacgt acggccattt tccttcggga acatcctgtt ttttcaggta tgtttttccg
     2221 aattcaaaaa ggcgcaggtc ttcgcttttc cggttacggt tgtacgcgat cgtttcgagt
     2281 ccgccgaaca gcagcgaccg gcgcatgatg ccgaggtcgg agctgagcgg gttgagcagc
     2341 gccacgttcg atgaagcgtt gttcccttcg agcaggtcgt aatacgctgc tttcgtcagc
     2401 gagttgttga gcatctcacg gaaaccgttt gccgcaagca ggtcagcaat ggcatcattc
     2461 accttttccg catccggttt cggggagtac gaaagcgaag cgcggagaaa atccgggaag
     2521 ccgatgttgt tgtaaccgta cacgcgcagc acttcctcca cgacatccgc atcgcgcgtc
     2581 acatccacct tgaacggggg aacggacagc agcaatgcat ctttcccttc ggaaataatt
     2641 tccatgccca acgcaagaag tatcttccgc acgatcgcgt gctcaatccg tttcccgatg
     2701 agcgtttcac atttcgcgta cgtgaacgcc acctgtttcg gctcgacctt caccgggtaa
     2761 atgtccaccg cttctgccgc aaccgatgcg ccggcgactt cctcaagaag atacgccgcg
     2821 cgctgcaggg cgaacatcgt catttccgga tctgttccgc gttcgaaacg gaaagaagaa
     2881 tctgttttga gcgaatgacg tttcgacgtt ttacgcacgt gaacgggatt gaagcatgcg
     2941 ctctcgagaa agatcgtttt cgttttttcc gtcacgccgg aatcgagtcc gccgaaaaca
     3001 cctgccatgc acatgggttt tccggcatcg cagatcatga gatcgttcgc ggaaagtttc
     3061 cgttccgttc cgtcgagcgt gacgaacttt tctccttcat tcgccttgcg cacgacgatc
     3121 ttgtttccgc tgatcctttc cgcgtcgaat gcgtgcagcg gctggcccaa ttcatgcaga
     3181 acatagttcg tcacatcgac aacgttattg accgggcgca cgccgatcgc tttcagtcgc
     3241 tgctgcagcc attccgggga atctttcact tcgacaccgg tgagataaat gcctgaataa
     3301 cgcacgcagg cttccgtgtc ttttacgtcg acagaaacgg gagagaccgg aagcccgtct
     3361 tcaaaagtaa ccgccggcgg gagctgtgcc agaacatgtt cagcagaaac atctttcagc
     3421 agttcagcgg ccagcacgac ggcggaaaga tcgcgcgcca caccgaagtg cgacgcagcg
     3481 tccgcccggt tcggtgtaag accgatctcg aggacgtgat ctgtttttat cccgaaatat
     3541 tccgtcgcag gcgttcccgt ttttgcggaa ggatcgagca ccatgatgcc ggcgtgtgaa
     3601 gacccgagac cgatctcgtc ttcggcgcag atcattcctt ccgaaagctc gccgcgtatt
     3661 tttgatttct taatctcgaa cggctcgcct tccaccgggt gaaccgtggt tcccacggtg
     3721 gcgacgatca ctttctgtcc tgcttgcacg ttcgacgcac cgcacacaat gctgagcagc
     3781 tccgttccgc ccacatccac tttcgtcagt gaaagcttgt ccgcgttcgg gtgcttcgtt
     3841 ttttccatca cttctcccac gacgagacct ttcagtccgc cgggtatcga ctcaaacgtt
     3901 tcgacacttt ccacttcgag tccgcagccg gtgagcaggg ccgcaatttc ttccgcgctt
     3961 ttatccgacg ggaaataatc gttcagccag ttcagagaga ttttcataca ggtaaaatga
     4021 gcttcgaaat tacgtgaaaa tccgggaacg gttttgcggg agaaggccgg ggcagcaacc
     4081 cgcttccgga agaaaccccg gttctttgcg gtcgcggcat tcacggcaaa agaacaggcc
     4141 ataaaaaaac gtgctcgccc aaagaaaatc caacccgatt gccggaaggt cgcacgatcc
     4201 gctcgttgcg aaagatcatt ccccggacag ttttgaaaca agacccgttt aagccagggc
     4261 accgccatgc tacagtgacg attttgtttt gcttacgggt ttcgccatta tcatttccac
     4321 tttttcatat acggtttttg gataacttta atacaaatga aaaagctaac cctgtacgcc
     4381 cttgtcatga ccctgctgct tttttcgtgc agcatggaaa agcgcattta ccggaatggc
     4441 tggtatgtcg accgtcattc aacggcaaca acaaaaggaa atgtgcagca ggcgaacgat
     4501 gataaaactt tcaccgccgc tgcacccgtt cccacgcagg acaccctgca gccgtccccg
     4561 gattccacca gcctgaaaaa aacaaccgtt gatgattcaa cgaattctcc gccttccttc
     4621 cgtgagttct tccgctcaat ggaaaataaa gtgcttccgc ccggcgacgg ggacggacgg
     4681 cccatgaggt attccgaagc gcgcaggagc atgaaggata aaggctgccc gcccaaccag
     4741 atggcccaga ccgtttattt catggcgtgg gcctccattg cggcgacatt cctcggcggc
     4801 gcggggattc tcctggcgat catcacgctc atcctttcca ttttcgccat gcagaatgtc
     4861 tatgataatc cggacgattg cgtggacgaa aatatcgcga tcatcaaagc ggcgcgcagg
     4921 atagcgctta tctacctgct ggcggcagta gtggcgggcc tggcggcttt cgttatactg
     4981 ctgtgggcga tcagcatgct gtaataaaat gatcatacgg ggaaggagga acggcccgtc
     5041 ctgtccagcg ttttctgaat attgtcagca accgccggca tttttctata ataatcagct
     5101 cccggaagaa aaggaaagtg ttccccgtgt acctttggca cttccggatg aattccactt
     5161 ttcgtaaact caataaccgc gcaggctggg tagtttttat cctcgccgcg atcgtcttct
     5221 gcagctgcat cgagcctaca gccagcttct gggattgcag cgaatacatt gcctgtgcga
     5281 acgggctcga aacggggcat ccgcccgggg cacctttctt ccttcttatc gcaaaatttt
     5341 tctcgctgtt ttctttcggc gataaaacgc tcgtcgcccc gctggtgaac atcatgtccg
     5401 caaccgcgag cgcattcacc gtgcttttcc tgttctggac catcacgcac cttgcacgga
     5461 aaatcatgct gcggaaaaac gaagaccaca cttcctgtaa aatgtggctc gtcatcgccg
     5521 ccggcgccat cggtgcgctg tcgtatgcct tcacggattc tttctggttc tcggccgagg
     5581 aaggtgaagt gtacgcgctt tcctctttct tcacggcgat cgttttctgg gccatgctca
     5641 aatgggaaga catggccgat gatccgcatt ccgaccggtg gctggttttc attgcgtaca
     5701 tggtcggact ttccgtcggc gtgcacctgc tcaacctgct gacgatacct gccatggtgt
     5761 ttatcggtta tttccggaaa acaaaaagaa taacgtggtg gggattttcc ctcgccggca
     5821 tctgctccgt gcttctgctc ggcggggtcc aggatatgat cattcccggt atcgtcaatc
     5881 ttgcgggtaa aacggaactg tattttgtca atgatctcca tttaccgttc aacagcggaa
     5941 ccattttcta tttcggtacg ctcctcctgc tgctcattgg aggaatcgcg ttttcgcacg
     6001 gaaaaaagat ccgctggctg aacacaacgc tcattgcatt caccgtgctg ctgatcgggt
     6061 acagcagctt tttcgtgctt gtcatccgct cgcaggccgg aacgccgatc aacgaaggtt
     6121 ctcccacgaa cgcgatcagc atgctttcgt acctcaaccg tgagcagtac ggcgactggc
     6181 ccatccttta cggccagtat tacaattccc cgctcgatgc ggagcagccg tatctcgacg
     6241 gcaacccggt ttacgtgaaa gatgcggcga aaggaaaata cattgtcagc gacgcgcgca
     6301 aagcatccgt cccgaattac gatccgaaag gctgcacgta ttttccgcgc atgtacgatt
     6361 ccggtcacgg cgaaggatat tcctcctggg ctgacgtgca gggaacgccg acgacattca
     6421 gcgacaaggg tggaaaaaca ttttcggaga acatcccgac gttcggcgag aacatgcgtt
     6481 ttttcatcac gtatcaatgc gggtggatgt acatgcgtta cttcatgtgg aatttttgcg
     6541 gacggcagaa cgaccgtaca ggctatggaa atgacgcgga aggaaacatg ctcacctgca
     6601 ttccgttcgt cgacaatgaa cgcgtgggca accaggatct tctgccgcct tcccagaaaa
     6661 acaacaaagc gcacaaccgt tatttcctta ttccgctgct gctcggcatc ctcg
//
LOCUS       DASTSF010000578         9301 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1182507,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000578 DASTSF010000000
VERSION     DASTSF010000578.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 9301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9301
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1182507"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            240..1058
                     /locus_tag="VFU15_16340"
     CDS             240..1058
                     /locus_tag="VFU15_16340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020527807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEU4719416.1"
                     /translation="MSFSQQFIHLFSIGGLVSLLTLTVLEIVLGIDNVIFISIIAGKV
                     KDKKERRRARGIGLLLALVMRIALLFGITWIIGLTQPLFTFNSLIDFFRTMGLEHPEA
                     AAAVTGRDIILFCGGVFLLAKTTSEIHEKMDMAGDTEEEKKNKASSVLSVVLQIMFID
                     IVFSFDSILTAVGIVNEVVIMIAAVVVSMMIMLAFSEKVSDFIERHPTVKMLALAFLL
                     MIGFLLILEAFDIHVPKPYVYCSMGFAFLVELLNMRLRKKTAKKAAAKAAQKKE"
     gene            complement(1340..2035)
                     /locus_tag="VFU15_16345"
     CDS             complement(1340..2035)
                     /locus_tag="VFU15_16345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEU4719417.1"
                     /translation="MNCLVIDDNALSRTALRQLISQTDFLELKGECADPVEAFNALKK
                     EKIDLLFLDIEMPGMTGLEFVRQLETRPIIILVSAKKDYALEAFELNVADYIMKPVQL
                     PRFMTAVARAKELFDNRESKVEKSEDREYFFVRTHKQLSKIRLDEILYIQALGDYVTI
                     HTFEKKITVHLTLRAIENELPSARFYRLHRSFIAALDHIDSIEENTAYIKGNPVPIGE
                     PYKSTLLKKLHLI"
     gene            complement(2046..2885)
                     /locus_tag="VFU15_16350"
     CDS             complement(2046..2885)
                     /locus_tag="VFU15_16350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013668398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA-like polyprenyltransferase"
                     /protein_id="HEU4719418.1"
                     /translation="MSLIVFSHTIFAMPFAVIGFLLAVHTPGASFSWIKFALVILCMV
                     FARSAAMGFNRYIDRHIDAKNNRTAAREIPAGVISPASALAFVIIASVLFVLATYFIN
                     TTCFYLSPVALLVVLGYSYTKRFTALCHVILGTGLSLAPIGAWLAVTNSFALLPLLFS
                     FSVIFWVSGFDMIYALQDEEFDRSNRLHSVPALLGKKGALRVSELFHVISASFIWFAG
                     WYAGFGFFYWTGAAVYSALLIYQHSIVKPDDISRVNRAFGTTNGIASVVFAAFVILQI
                     YFG"
     gene            complement(2921..3178)
                     /locus_tag="VFU15_16355"
     CDS             complement(2921..3178)
                     /locus_tag="VFU15_16355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719419.1"
                     /translation="MEKIVRVLGWAGFACGAITAVFATMPGRWGLIAMFIMLPGFLLS
                     SAYVLLSTRYRIESKFFNPGYAGMILSSVPLMLIIYFMLTK"
     gene            complement(3214..5352)
                     /locus_tag="VFU15_16360"
     CDS             complement(3214..5352)
                     /locus_tag="VFU15_16360"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HEU4719420.1"
                     /translation="MKKILLLILLLPSFCFAQKSWNIPLSLYNKSVMLPPGQLMGVLV
                     EGNPVEVAEAAKKYGGALHTNYGDICSVAIPAGKLVAFSREPGIIRFGNAPLKMQPLN
                     DTMKVQTRITDVHNGLAPLTQPYFGDGVIMGMIDSGIDFNHPDFKDSLGRTRILYIWD
                     QRDTTGPGPAPWNYGTLWDSAQINAGQCTHNDLAYYGHGTHVTGIAAGNGRSTTARDY
                     SGGASHSDIIMVALDFYGIYSAVSVADAAAFIYEKAQSLGRPCVINASVGDYGGSHDG
                     QDLMALMIDSLLDTPGRAFVCAVGNAGNEPIHLSYPLSSDTNFTWFVNTANGFQLQLW
                     ADTNNFNNAHFNIGCTRESDWHDIGSNAFTDIQSNLGIYSTDTIKNSAQQRIALVYRY
                     GSIQGSAYSQEILVVPDSSNGYDFSLNLTGSGLFHLWSFDYKPNNFPTTGQYPKMVYY
                     KQTDTTHTVCSSFTCSQRTITVGNYVNRSTWMDVNNNWETDTTVTAGAIMWNSSVGPT
                     RDGRQKPDITAPGANDISCAVISTLPGFISGIPQYVGVGGFHIVGGGSSASSPVVAGS
                     VALYLQLHPAANWQDCHDAVVFCAKQDTFTGTNLPDYTWGYGKMDAFSMMTNCALTTQ
                     STQPPAQNDFLVFPNPAAPGQSLMMTFPAVSVSSQLEIFSADGQLVWSQQVQGGEASV
                     VIPAGLLSPGLYLVRLSGAGSQRTQKLIVN"
     gene            5477..6109
                     /locus_tag="VFU15_16365"
     CDS             5477..6109
                     /locus_tag="VFU15_16365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719421.1"
                     /translation="MKMCALLVLLLLPVKIFAQDSLKYSPDFSFTEGIYLSFQQFRDN
                     RPVPKSKIVSDYDTTKLNFIHQVVSGKTMRYLDADGHLVEINPGKLWGYSENNSVFIH
                     FNGEFNRIVVIGSICHFTAYYTTYATTGPTMNGPDYGAPVENLRQYILDMQTGLVHDF
                     VLEEMEPILQRDDAIYKEFMALRKGKRRKLMFFYLRKYNEAHPLYFKTPA"
     gene            6106..7302
                     /locus_tag="VFU15_16370"
     CDS             6106..7302
                     /locus_tag="VFU15_16370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719422.1"
                     /translation="MKNFAAFFLLLLCGTAYGRTDSLANKLQVEGSIGMGRVSGRQTQ
                     QALSLCAAYRTITGGVQMLRSGKSDGYLFENDFLLGKRFYFGRMLALTMSAGYADIAF
                     ETGHHTGDIVPQNFTKAGGFVFDAEADLAIPRPARRGASAWSLYAAYYANRNAKQNLD
                     GYTFGVKFCPWRYVPMAERRKEADRQPGGKKKKDGQVADDNAPDLYHYVFAGVSTFDL
                     DVMAGYAMMRPAHPSKGYTFYGGWRSFSMLTHTFSSFVYPDRTLKGPMLGFGFDWYTS
                     GKHGVREASVVFMTRALYADHVWVGTMDADRGTEWLISRERYDLCVLGRIGRRTSSLD
                     HFFSGIYIAAGPRLSVYHTSFYRSSFTGSVSDPHPAPDEGFQWREKGPDAGFEFHVQL
                     FFGGGW"
     gene            complement(7309..7755)
                     /locus_tag="VFU15_16375"
     CDS             complement(7309..7755)
                     /locus_tag="VFU15_16375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719423.1"
                     /translation="MEKPRMNNPCPQDWNKMSPSATGRFCAKCCKVVVDFTQKTAEEI
                     RDYLAANAGTCGRFSLSQLQPLRQRVMQKVSWRMRRFSLALYLIFGSLLFTGTACGGE
                     VDYYGKQDSAYSAQQSAHRNDSLKKDSVLRMDSHKKDSAQETKKHH"
     gene            8652..>9301
                     /locus_tag="VFU15_16380"
     CDS             8652..>9301
                     /locus_tag="VFU15_16380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005856590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU4719424.1"
                     /translation="MSEGRQIIFSMVNVSKIHPPQKQVLKDIYLSFYYGAKIGVIGLN
                     GSGKSSLLRIIAGVDKDYIGEIHFSPGYSIGFLEQEPKLDESKTVMDIVREGVKEIND
                     TLKEYEEVNNKFAEPMSDEEMNKLIEKQARLSEKIELLDAWNIDSRLERAMDALQCPD
                     GDTPISVCSGGERRRVALCRLLLQQPDILLLDEPTNHLDAESVQWLENHLQNYPGT"
BASE COUNT         2111 a         2409 c         2666 g         2115 t
ORIGIN      
        1 aatattcatt tctcggtttt tttcgaatcg gtttatgcta aaccctcaac ttgacgaagc
       61 aaagatatat gtatctgttg gtactatgca atacaaaata ctgtatgaat ttttaattta
      121 ttgaaaatca gtactataaa attagaatgg aataagaaaa acccggttcc gcagcaggaa
      181 ccggtctctt tagacgcccg tttttgcgtt acatttgttc ccgcgagcga ctaaagcgta
      241 tgagtttttc ccagcaattc attcatttat tttccatcgg cggactggtc agtcttctta
      301 cactgacggt gctggagatc gttctcggga tcgacaacgt catctttatt tcgatcatcg
      361 cgggaaaagt aaaagacaaa aaagagcgcc ggcgcgcgcg cgggataggc ctgctgctgg
      421 cgctggtcat gcgcattgcg ctgcttttcg ggatcacgtg gatcatcggt ctcacgcagc
      481 cgctgttcac gttcaattca ctgatcgatt ttttccggac gatgggcctc gagcaccccg
      541 aagcggcggc ggccgttacg ggacgtgaca tcattctttt ctgcggcggt gtttttcttc
      601 tcgcgaagac gacgtctgag atccatgaga aaatggacat ggccggcgat acggaagagg
      661 aaaaaaagaa caaagcgtct tccgtgcttt cggtcgtgct gcagatcatg ttcatcgata
      721 tcgtcttttc tttcgattcg atcctcacag ccgtgggaat tgtgaacgaa gtcgtgatca
      781 tgatcgcggc ggtggttgtt tccatgatga tcatgctggc gttctccgaa aaagtgagcg
      841 atttcatcga gcgtcatccg acagtgaaaa tgctggcgct tgcgttcctg ctcatgatcg
      901 gcttcctgct catcctcgaa gcgttcgata ttcacgttcc caaaccgtac gtgtactgct
      961 ccatgggatt tgcattcctc gtcgagctgc tgaacatgcg cctgcggaaa aaaacggcga
     1021 agaaggcggc agcgaaagcg gcgcagaaaa aagagtagga tttttttacc gcgagatccc
     1081 cggagaaaga attcaggatt aatgacgaat ggggagacct tcattcgcgg tttcgcactt
     1141 cctgcggaag ctcgcccgga tttttagtct gcgctaactt ttaccacctc actgtccctg
     1201 tttcgtaatc gtcgccggaa ctcaatttcc ggtcgaagcg ggcggacatt cttttgtgcg
     1261 cttttcagtt tttcaatgca gaaaattgca ggcgcttctg atcgcggaac aacagggtac
     1321 tgtgcggaga attcgcatct cagatcaggt gcagcttttt cagcagcgtc gatttgtacg
     1381 gctcgccgat ggggacggga tttcctttga tataggccgt gttttcctca atggaatcga
     1441 tgtggtcgag cgcggcgatg aaggaacggt gcagcctgta aaaacgcgcc gacggaagct
     1501 cgttctcgat ggcgcgcaac gtcaggtgga ccgtgatctt tttctcgaac gtgtggatgg
     1561 tgacataatc gccgagcgcc tggatgtaga gaatttcatc gaggcggatc ttcgacagct
     1621 gcttgtgcgt gcggacgaaa aaatattcac ggtcttcgct tttctccact ttcgattcgc
     1681 ggttgtcgaa caattctttc gcccgcgcga ccgccgtcat gaaacgcgga agctggacgg
     1741 gtttcataat gtaatccgcc acgttcagct caaaagcttc gagcgcataa tctttttttg
     1801 ccgagacgag gatgatgatc ggccgtgttt ccagctggcg gacgaactcg agcccggtca
     1861 ttcccggcat ttcgatgtcg agaaaaagca ggtcgatctt ctctttcttc agcgcattga
     1921 acgcttcgac cgggtcagcg cattcgcctt tcagctcgag gaaatcggtt tgcgagatga
     1981 gttgccgcag ggcggtgcgg gaaagggcgt tgtcgtcgat gacgaggcag ttcataaatg
     2041 tttttttagc cgaaataaat ttgcaggatg acaaacgccg cgaacaccac gctcgcgatg
     2101 ccgttggttg tgccgaacgc gcggttcacg cggctgatgt cgtccggctt tacaatgctg
     2161 tgctggtaaa tgagcagcgc ggaataaacg gcagcgccgg tccagtaaaa aaatccgaag
     2221 cccgcgtacc acccggcgaa ccagatgaac gaagcggaaa tgacgtgaaa tagttccgaa
     2281 acacgcagcg cgcctttttt cccgagaagc gccggaacgg aatgcaggcg gttgctgcgg
     2341 tcgaattctt catcctgcag cgcgtagatc atgtcgaacc cgctcaccca gaagatcacg
     2401 gaaaacgaaa aaagaagcgg aagcagcgca aaggaattcg tcaccgccag ccacgcgccg
     2461 atgggcgcaa gtgaaagccc ggtgccgaga atgacgtggc acaaagcggt gaaccgtttc
     2521 gtgtagctgt agccgagcac gacgagcagc gcgaccgggg aaagataaaa gcaggtggtg
     2581 ttgatgaaat acgttgcaag aacgaacagc accgaagcga tgatcacgaa cgcaagcgcc
     2641 gaagcaggag aaatgacacc ggcgggaatt tcacgagccg ctgtccggtt gttctttgca
     2701 tcgatgtggc ggtcgatgta acggttgaat cccatggcgg cgctgcgcgc gaagaccatg
     2761 cacaggatga cgagcgcgaa ttttatccag gagaacgaag cgcccggcgt gtgcacggcg
     2821 agcaggaacc cgatgacggc gaacggcatc gcgaagatcg tgtggctgaa aacgatcagc
     2881 gacatgtagc tggatacggc cggtttcgct gtggttgtgt tcacttcgtc agcatgaaat
     2941 aaataatgag catcagcgga acggagctca ggatcatgcc cgcgtaaccg ggattgaaga
     3001 atttcgattc gatgcggtaa cgcgtgctca gcagcacgta tgcgctcgaa agcaggaagc
     3061 cgggaagcat gatgaacatc gcgatgagtc cccagcgccc gggcatcgtg gcaaagaccg
     3121 cagtgatcgc gccgcatgca aacccggccc agccaagcac ccgaacgatt ttttccatgg
     3181 cgcaaaggta agctgacatt ccgccggtga agctcagttc acgattaatt tctgcgtgcg
     3241 ctgcgaacct gcgccggaca ggcgaaccag gtaaaggccg ggtgaaagaa gtccggcggg
     3301 aataaccacg ctggcttcac cgccctgcac ctgctgcgac catacgagct gcccgtccgc
     3361 ggaaaaaatt tccagctgcg atgaaacgga aacggccggg aaagtcatca tcagcgattg
     3421 gccgggtgct gcgggattcg ggaagacgag gaaatcgttt tgcgccggcg gctgcgtgct
     3481 ttgcgtcgtc agcgcgcagt tcgtcatcat gctgaatgca tccattttcc cgtaacccca
     3541 ggtataatcc gggagattgg ttcccgtgaa cgtgtcctgt ttcgcacaga aaacaactgc
     3601 atcgtggcaa tcctgccagt tcgccgccgg gtgaagctgg agatacagcg cgacgcttcc
     3661 ggcaacaacg ggcgatgaag cggaagagcc tccgcccacg atgtgaaatc cgcccacacc
     3721 gacgtattgc gggatacccg aaatgaatcc cggcagcgtg ctgatcactg cgcaggaaat
     3781 gtcgttcgca ccgggagcgg tgatgtccgg tttctgcctg ccgtcgcgcg tgggaccaac
     3841 gctcgaattc cacatgatcg cgccggcggt gacggtcgtg tccgtttccc agttgttgtt
     3901 tacgtccatc cacgtcgacc ggttcacgta attaccgacg gtgatcgtcc gctgcgagca
     3961 ggtgaagctg ctgcagacgg tatgcgtcgt gtcggtctgt ttgtaataaa ccattttcgg
     4021 gtactgcccg gtggtcggga aattattcgg cttgtaatcg aagctccaca aatggaaaag
     4081 tcccgaccct gtgaggttga gtgaaaaatc atagccgttc gaggaatccg gcaccacgag
     4141 aatttcttgg ctgtacgcgc ttccctgtat ggatccgtag cgatacacga gcgcgatgcg
     4201 ctgctgcgcg gagtttttta tcgtgtcggt ggaatagata ccgaggttcg actggatatc
     4261 agtgaatgca ttcgagccga tatcgtgcca gtcgctttcg cgcgtgcagc cgatattgaa
     4321 atgcgcattg ttgaaattgt tcgtatcggc ccagagctgg agctggaaac cgttcgccgt
     4381 attgacgaac caggtgaagt tcgtgtcgct cgaaagcggg tacgacaaat ggatcggttc
     4441 gttgcccgca ttcccgacgg cgcacacgaa tgcgcgtccc ggcgtgtcga gcaggctgtc
     4501 gatcatgagc gccatgagat cctgcccgtc gtgcgagccg ccataatcgc caacgctggc
     4561 gttgatcacg cagggacggc caagcgactg cgctttttca taaatgaaag cagctgcatc
     4621 cgcaacggaa acggcagaat agatcccgta aaaatcgagc gccaccatga tgatgtcgga
     4681 atgcgaagcg cccccgctgt aatcgcgtgc agtagtcgag cgcccgttgc ctgcggcaat
     4741 tcccgttacg tgcgtgccat gaccgtaata ggcaagatcg ttgtgcgtgc attgtcccgc
     4801 gttgatctgc gcgctgtccc acaacgtccc gtaattccac ggcgcgggcc ccggccctgt
     4861 cgtatcgcgc tggtcccaga tgtagaggat gcgcgttctg cccaatgaat ctttgaagtc
     4921 ggggtggttg aaatctatgc cggaatcgat catgcccatg atgacgccgt cgccgaagta
     4981 cggttgcgtg agcggggcga gcccgttgtg cacgtcggtg atgcgcgtct gcactttcat
     5041 cgtgtcgttc agcggctgca ttttcagcgg agcgtttccg aagcggatga tgccgggctc
     5101 gcgggaaaaa gcgacaagct tgccggcggg tatcgccacc gagcagatgt cgccgtaatt
     5161 cgtgtgcagg gcgccgccgt attttttagc cgcttcagcc acttccacgg gattgccttc
     5221 cacgagcacg cccataagct gaccgggcgg gagcatcacc gatttgttgt agagggagag
     5281 cgggatgttc cagcttttct gtgcgaagca gaatgagggg agcagcagga taagcagtaa
     5341 gatttttttc atgggaaaac gcttgcgttg atcaattaaa gttacgataa acagtgcgga
     5401 tggtatgaga cagtgattaa aaatcttccc ggccgttcat ttcacccgga aaattattcc
     5461 gccgatattt ggcaggatga aaatgtgcgc tctcctggta ttgctgctgt tgcccgtgaa
     5521 aatattcgcg caggattcgc tgaaatattc tcccgatttc agcttcaccg aaggcattta
     5581 tctttccttc cagcagttcc gtgacaaccg gcccgtcccc aaatcgaaga tcgtttcgga
     5641 ttacgatacg acgaaactga atttcattca ccaggtggtc agcggaaaaa cgatgcgtta
     5701 ccttgatgcg gacggtcatc tcgtggagat aaacccggga aagctgtggg ggtattcgga
     5761 gaataattcc gtgttcattc atttcaacgg cgaattcaac cgcattgtcg tcatcgggag
     5821 catctgtcat ttcaccgcgt attacacgac gtacgcaacg acaggtccga caatgaacgg
     5881 ccccgattac ggagcgcccg tggaaaacct ccggcagtat attctcgaca tgcagacagg
     5941 tctcgtgcac gatttcgtgc tcgaagaaat ggagcctatc ctgcagcgcg acgacgcgat
     6001 ctataaagaa ttcatggcgc tgcggaaggg aaaacggagg aagctgatgt ttttctacct
     6061 gcggaaatac aacgaggcgc acccattgta ttttaaaact cctgcgtgaa aaattttgct
     6121 gcattttttt tgctcctgtt gtgcggcact gcttatggcc ggaccgattc gctggcgaac
     6181 aaactccagg tggaaggttc cattggcatg ggcagggtaa gcgggagaca aacgcagcag
     6241 gcgctgtcat tgtgcgcagc gtacaggacg atcaccggtg gcgtgcagat gctgcgttcc
     6301 gggaagagcg acggctatct ttttgaaaat gatttcctgc tcgggaaacg attttatttc
     6361 ggcagaatgc ttgcgctgac catgagcgcc ggttacgccg acattgcttt cgagaccggc
     6421 caccacaccg gcgacatcgt cccgcagaac tttacgaaag ctggcggatt cgtgttcgat
     6481 gcggaagccg atcttgccat cccgcgaccg gcgcggcgcg gcgcttcggc atggagcctg
     6541 tacgctgcgt attatgccaa ccgcaatgcg aaacaaaacc tcgacgggta tacgttcggt
     6601 gtgaaattct gcccgtggcg ctatgtgcca atggcggaac gccggaagga agcagaccgg
     6661 caaccgggcg ggaaaaagaa aaaagacggg caggttgcgg atgacaacgc gcccgacctg
     6721 taccactatg tttttgccgg cgtaagcacg ttcgatctcg atgtcatggc cgggtacgca
     6781 atgatgcgac cggcgcatcc gtcgaaagga tatacgtttt acggaggctg gcgcagtttt
     6841 tcgatgctta cgcacacctt cagcagcttc gtttatcccg accggacgtt gaaaggtccc
     6901 atgcttggct tcggttttga ctggtacacg tccggaaagc acggcgtgcg ggaagcatct
     6961 gttgttttca tgacgcgtgc actttacgcc gatcacgtat gggtgggaac gatggatgct
     7021 gaccgcggca cggaatggct tatttcacgc gaacgctacg atctttgcgt gctgggacgt
     7081 atcggccgca ggacatcatc actggatcat tttttcagcg ggatatacat tgctgcggga
     7141 ccgcgattat ccgtttacca tacgagcttt tacaggagct cattcaccgg cagtgtttcc
     7201 gatccgcatc ccgcgcccga tgaaggattc cagtggaggg aaaaaggtcc cgacgccggt
     7261 ttcgaattcc acgtgcagct tttcttcggc ggcgggtggt gaggctgatc agtggtgctt
     7321 tttcgtttcc tgcgcggaat cttttttgtg cgaatccatt cgaagcacag aatctttttt
     7381 gagcgaatcg ttgcgatgtg cggactgctg cgcggaatag gcggaatcct gtttcccgta
     7441 atagtccact tcgccgccac aggccgtgcc ggtgaagagg agcgagccga aaatgaggta
     7501 cagcgccagc gagaaccggc gcatgcgcca ggaaactttc tgcatcacgc gttgcctcag
     7561 cggctgcagc tgcgaaaggc tgaaacgtcc gcaggtgccg gcattcgcgg caagataatc
     7621 gcggatctct tccgccgttt tctgtgtgaa gtcgacgaca accttgcagc atttggcgca
     7681 gaaacggccg gtggcggagg gcgacatttt gttccagtcc tgcggacagg gattattcat
     7741 gcggggtttt tccatgggaa gggttgggtg ttgtgtaaga tgggaactgt tctcgactgg
     7801 cgctcgaact gacgaatgac tgcgctcgaa ctgttctcga ctgcgctcga actgacaggt
     7861 gactcgcgct cgaactgaca ggtgactctc gctcgaactg acgggtgact gcgcccgaac
     7921 tgttctcgac tcgcgctcga actgacgggc gactgcgccc gaactgttct cgactctcgc
     7981 tcgaactgac gggcgacttg cgctcgaact gttctcgact ctcgctcgaa ctgacgaatg
     8041 acttgcgccc gaactgttct cgactctcgc tcgaactgac gggtgactgc gcccgaactg
     8101 ttctcgactc tcgctcgaac tgttctcgac tggcgctcga actgacgaat gactcgcgct
     8161 cgaactgacg aatgacttgc gctcgaactg acgggtggct ggcgcccgaa ctgttctcgg
     8221 ctggcgcccg aactgacggg tgacttgcgc ccgaacggac agtgattgat tgtacatgca
     8281 gtgtttccgt gtctcgtgcg attaaaaata agcacacttt ttttgagttg aaacaagtta
     8341 acaagcgttg tgaatttgaa gcgatgaagg aaagaatgca tagccgcggc tgagcgggta
     8401 gcctgcgcgg ccctccaccg gccttggtct tgcgctcagg ctgacgagga ggccgcgcag
     8461 ctacgagcga agacgggaaa acagggaata caatgagaaa ccggtgcgct ccctgtcaaa
     8521 aaaacaaccc gggaatgctg cgttccgcgc ggggccgata ttccattagc attttgcctc
     8581 attttacgcg atatttgcac ccttaaccgc aagcggattt ttattcattt tcagaaccaa
     8641 tacactctcc catgtcagaa ggaaggcaga tcattttttc aatggtgaat gtttccaaga
     8701 tacacccgcc gcagaagcag gtgctgaagg acatttacct ctctttttat tacggcgcga
     8761 agatcggcgt gatcggtctg aacggttcgg gaaaatcatc cctgctgcgc atcattgcgg
     8821 gcgtcgacaa ggactacatc ggtgaaatac atttttcgcc cggctattcg atcggttttc
     8881 tcgagcagga accgaagctg gatgaatcga aaaccgtgat ggatattgtg cgtgaaggcg
     8941 tgaaagagat caacgataca ctgaaggaat acgaagaggt gaacaacaag ttcgccgagc
     9001 cgatgagcga tgaagagatg aacaagctca ttgaaaagca ggcgcgcctt tcggaaaaga
     9061 tcgagctgct ggacgcctgg aacatcgatt cccgcctcga gcgcgcgatg gatgcgctgc
     9121 aatgccccga cggcgatacg cccatttccg tctgctccgg cggtgaacgc cgccgcgtgg
     9181 cgctgtgccg cctgctgctg cagcaacccg atattctcct gctggacgaa ccgacgaacc
     9241 acctcgacgc cgaatcggtg caatggctgg aaaatcattt gcagaattat cccggcacca
     9301 t
//
LOCUS       DASTSF010000579        10753 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_560759,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000579 DASTSF010000000
VERSION     DASTSF010000579.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 10753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10753
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_560759"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1624)
                     /locus_tag="VFU15_16385"
     CDS             complement(<1..1624)
                     /locus_tag="VFU15_16385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719425.1"
                     /translation="MRFSVFFIALLLFPFVKMDGQVTDAWREKNVAVSGDTVVLDTLS
                     IVPGTFSVLSGGTPLDTAKFTLLPAEAQLILKPGVPRDSLHVKYAVFPVLFTHSFSHK
                     NQQQFTSRPDHADNPFLYRPGDAKADDPFATGGLTKSGSLSRGISFGNTRDLSVNSAL
                     NLQLSGKLTDNIDLTMAATDDNLPIQPDGTTQQLQEFDRVYIQLSGKGTKLIAGDFFT
                     SRPNSYFMNFNKRGQGLNITSVIPLDEKDPKKTFGVSGSIALSKGKFARNVIQGVEGN
                     QGPYRLTGAENELFIIVLSGTEKVYLDGKLLTRGQDNDYIIDYNTAQITFTAKQLITK
                     DKRIVVEFQYSDRNYSRSLVHTGFEYKSDKFALRFNAYSEQDSKNQPLQQTLTDADRL
                     IMAAVGDTLLKAVVPGVDSVPFSGDLVLYKKVDTLVGATLYRNVYVYSTSSDSAHYRL
                     SFSYVGANHGNYIQYSTSANGKTFRWIAPVSGVPQGDYEPVILLITPKKKQLVTFGGD
                     VQLSKNTFFTGELAYSSNDLNTFSSYDSFDDKG"
     gene            1748..2515
                     /locus_tag="VFU15_16390"
     CDS             1748..2515
                     /locus_tag="VFU15_16390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013634163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEU4719426.1"
                     /translation="MKIISYNVNGIRAALGKGWIDWVKKEDPDVICLQEIKAEPGQLD
                     LSVFEKAGYENHFWHPAEKKGYSGVAILSKEKPDNVITGSGNKTYDSEGRILRADFGD
                     LSVMSLYMPSGTTGDVRQDFKYGFMDFFQTYIDKLKKTRPNLVISGDWNICHRAIDIH
                     NPVSNANSSGFLPPEREWIGRFIDSGFVDSFRHFEPGPHHYTWWTFRANARARNLGWR
                     IDYHMVSRPLEKKLKAASILPEAKHSDHCPIVVELNY"
     gene            2641..4431
                     /locus_tag="VFU15_16395"
     CDS             2641..4431
                     /locus_tag="VFU15_16395"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU4719427.1"
                     /translation="MKRLVFTLAFSALAASVVININGCGPGDEGNGQERKANGPVVHY
                     GGVFRMNETEDFRTLYPLNIVEVVGDHIGSQVYEGLVKFNQKDLSIMPGLATRWDIKD
                     SSTHFIFHMRKGVFFHDDPCFPGGKGREVKASDIKYCFDMLCTASPNNQQYSNTFKDR
                     VAGANEYFASTDSKKPLAGGVSGVTVPDDSTVDIRLVRPMPGFLNILATQGCWIYPQE
                     ALAKYGDEMRVHCVGTGPFRVKSIKEGDAVILEYNPKYWMFDEWNNRLPYLDIVEFRF
                     IKDKRSEIIAFKNKELDMVFRLPVEMANDIMGELSQAKEHGADFVFQSNPAMSLTYYG
                     FQNKLSPFDNVKVRQAFNYAINREYICEKVLMGDAVPAVYGIVPPSLPGYDAKTINGY
                     NYDPDKAKQLLAEAGYPGGKNFPATTLYINNGGGDRNTMTADYVRKQLNDVLGINVTV
                     ELVPFAQHLENIETGKALFWRTGWIADYPDPETFLTTLYGKHIPATMNERSSLNSVRY
                     SSPLFDSLFEAAANEPDAVKRTDLYHRADQVAMSDAAIMPIYYETNDRLVQKNVRGFD
                     INPMEYRDMTRVYFAPDENATGAKKDTAQK"
     gene            complement(4477..5196)
                     /locus_tag="VFU15_16400"
     CDS             complement(4477..5196)
                     /locus_tag="VFU15_16400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719428.1"
                     /translation="MEKLNKDWITEKSIDFEYKKYLLLAYLQHVDECFRAVKLYPPLA
                     DLISHYRFAKTLKENKEQLASLFPQRLTGFDAEAFRLNYEKVVSDGKVMEEIESILDF
                     SLPRFAGHVNEGMQLFDFIEDGIEMQPVGLLPLDVSCGYLFLRNAASKTNVYSWSVKL
                     FEEPGSTWRALHTSFFGSYSYSITHTYEFIKTELVKAVPDIPNPAVYAAETRLEIPVE
                     ETFLPVARRLLMKELASSSKS"
     gene            5355..5744
                     /locus_tag="VFU15_16405"
     CDS             5355..5744
                     /locus_tag="VFU15_16405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719429.1"
                     /translation="MRIKISALALLAAVALTTCVPARKYDEEKQKREEAEKQLADLKA
                     QNQDYETKNKELDQQLADSKKQVAGLISDTSGCGMRYRLLTSQYDQLTANYELLLKQN
                     KDLMARQSNENTQLVGKLNMTQEQLIQ"
     gene            5838..6356
                     /locus_tag="VFU15_16410"
     CDS             5838..6356
                     /locus_tag="VFU15_16410"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEU4719430.1"
                     /translation="MKELSTALAQKDSVVTALKAKVKAALVGFEGKGLTVEEKNGKIY
                     VSMEEKLLFPSGSYKVSQDGMNALKSLADVLIANPDINIMVEGHTDDVPYKGTGDIKD
                     NWDLSVLRATSVVKILVADGVDPKRLTAAGRGEYFPVDPAKTPEARAKNRRTEIILTP
                     KLDEIFKVLDNN"
     gene            6424..7704
                     /locus_tag="VFU15_16415"
     CDS             6424..7704
                     /locus_tag="VFU15_16415"
                     /inference="COORDINATES: protein motif:HMM:NF019365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEU4719431.1"
                     /translation="MKNSVLSCCIFLAVISAANAQTYSGKLGYGFSPPGFQLDFSQTG
                     SFLQEVAGFCGGGVVYANTDWRNDSASGGIIPQLQKVVAQLQPSPFAYTDMLTFAWAD
                     YPAIHLASPSNPVNNWTNAQTRADFMTMLVHTADSLQPAYMFIGNEVSFYIEQDSLDY
                     LNFISFYESTYDSIKLHSPNTKVGTIFNYEHINGYGNLNGWTDKHWSALTLIDSTKLD
                     VIGFSLYPFLSVPTANAMPADYLDTMFARTGNKPVVITETGWPADSLYGTWVSSPQQQ
                     VDYVNQLFPKLAGQNVEDVNWLYLNYLIDTTSNVGPLIFKSVSLRDSLGNDRPALPLW
                     ESMCPLTGISPAQNEKTLAFFPNPSDGNFTVQDPQAANAQLRIDDAEGREVYSGILAA
                     GTNRVSLSLPDGFYIATVFTEGEYPVTQKIVIRH"
     gene            7714..8736
                     /locus_tag="VFU15_16420"
     CDS             7714..8736
                     /locus_tag="VFU15_16420"
                     /inference="COORDINATES: protein motif:HMM:NF014114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEU4719432.1"
                     /translation="MTDRRSFIRTIGIASAAMSLPSFARGQSSAVKSQNAGGALKPAR
                     LKPGDLIAFTAPAGAIFNDDSVQKATAAFEAQGFRVKHGKTLSEKFGYLAGKDDLRAK
                     ELQDLFSDKETKAIMAMRGGWGSARLLPLIDPTIIAASPKIFSGFSDITTLLAAFQVT
                     TGLVTFHGPVGNSSLEDFTMTHFLDLVANAASPELVQDDVTILAEGEAEGMLLGGNLT
                     VLCAMIGTPWMPELKDSLLFLEETEEEPYSIDRMLTQLTLAGVFTKTKGVVFGQCVKC
                     VAEEPEKSFTLDEVLRQKLSSLGIPVMTGFSFGHIKDKFTLPLGIRARMSTQRKSLQL
                     LESSVA"
     gene            8782..10230
                     /locus_tag="VFU15_16425"
     CDS             8782..10230
                     /locus_tag="VFU15_16425"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEU4719433.1"
                     /translation="MKRIFTFLLPLLFLSASFRAQPANDTVIFTQLGHFYYDTVVWRA
                     AYSPLIDRLGRPYVYTASVDLGMVTFDINDPMNPVPVDTIRPADLNGYKATFLAQDAD
                     TLFITTGGFQGFTQRAGLSIYDVTNPATPVLMDHWDTAAWAHGVSHIVLDGDYAFLSA
                     MNDGIIVLNISDKHNIRFVSHFVPTTTPNTSYAPHTRGLYLDNDTLIDADDGGGLRII
                     DATDKQNLVQIGAYLNPVPHATDTVYPFYNHVWCIGKHAWIPVDYAGIEVDDYSNPAS
                     ITTDAWSNIWNNTGISSWYGSDGHTNEIVYTGPTTNVLMVSGADSQVLAFDPSDPANP
                     RIMGWWGPPNTDNEAAWGVDEFNGLVVCGYLRDVGSNQPYYSTYGGIQLLSWNLLVTG
                     ENDNSPATSTLMISPNPTNDKATVALPASTDELFTINLIDVTGRVVQTQSADPKMNGR
                     NAEIDMTNLAPGIYTVCVHGKTSMWTGKVVKE"
     gene            complement(10505..>10753)
                     /locus_tag="VFU15_16430"
     CDS             complement(10505..>10753)
                     /locus_tag="VFU15_16430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009780639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA methyltransferase"
                     /protein_id="HEU4719434.1"
                     /translation="DSYTFSRRPVKLVWAEAFQNAWFAISWEKKIKKWSRVKKEALIN
                     SDWEALKMNAVCRNATNSKRLLDQPGYVPRDVWRRIGE"
BASE COUNT         2595 a         2908 c         2882 g         2368 t
ORIGIN      
        1 atcctttgtc atcgaagctg tcgtaggatg aaaacgtgtt gaggtcattc gaactgtacg
       61 ccagctcgcc ggtgaaaaac gtattcttcg acaactgcac atctccgccg aacgtgacga
      121 gctgcttttt cttcggtgtg atgagcaata tcacgggctc ataatcgccc tgcggaacgc
      181 cgctgaccgg ggcgatccag cggaatgttt ttccattggc ggaagtggaa tactggatat
      241 agttcccgtg gttcgcaccg acgtaggaaa acgaaagacg gtaatgcgcg ctgtcggaac
      301 tcgtggaata gacgtagaca ttcctgtaca aggtcgcgcc gacgagcgtg tccacttttt
      361 tgtacagcac gagatcaccg gagaagggaa cgctgtcgac accgggaacg acggctttca
      421 gaagcgtgtc gccgacagcc gccatgatga gacggtcggc gtccgtgagt gtctgctgga
      481 gcggctggtt tttgctgtcc tgctcggaat acgcgttgaa gcgcagcgca aatttatccg
      541 acttgtattc gaatcctgtg tgaacgagcg aacgtgaata attgcggtcg gaatactgga
      601 attccaccac gatgcgcttg tctttcgtga tgagctgttt tgcagtgaac gtgatctgcg
      661 ccgtgttgta gtcgatgatg taatcattgt cctgcccgcg tgtgagcagc ttgccgtcga
      721 ggtacacttt ttccgttccg gaaagcacga tgatgaacag ctcattctcc gcaccggtga
      781 ggcggtacgg accctggttg ccttccacgc cctggatgac gttgcgtgcg aactttcctt
      841 tcgaaagcgc aatgctcccg gaaacaccga acgttttttt cggatccttt tcatcgaggg
      901 gaatgaccga cgtgatgttg aggccttgcc cgcgcttgtt gaaattcatg aaatagctgt
      961 tcggccgcga ggtgaaaaaa tcgccggcga tgagcttcgt tcctttgccg gaaagctgga
     1021 tgtacacgcg gtcgaattcc tgcagctgct gcgtggtgcc gtcgggctgg atgggcaggt
     1081 tgtcgtccgt cgccgccatt gtaagatcga tgttgtcggt cagctttccc gaaagctgga
     1141 ggttcagcgc ggaattcacg gaaagatcgc gcgtattgcc gaatgaaatc ccgcgcgaaa
     1201 gacttccgct cttggtcaga ccgccagtgg caaacggatc gtccgctttc gcatcaccgg
     1261 gacgatagag gaacgggtta tcggcgtgat cgggccgcga agtgaactgc tgctggtttt
     1321 tgtggctgaa agaatgcgtg aacaggacgg ggaacacggc gtatttcaca tggagactgt
     1381 cgcgcggtac gccgggtttg agaatgagct gcgcctctgc gggaaggagc gtgaattttg
     1441 ccgtgtcgag cggcgttccg ccggaaagaa cgctgaacgt tcccggcaca atgctcagcg
     1501 tatcgagcac aacggtatcg ccggaaaccg caacgttctt ttcccgccag gcatccgtca
     1561 cctgcccgtc catcttcacg aacgggaaca gcagaagcgc aataaagaaa accgaaaacc
     1621 gcattgccga aactttaaag gtagcgaaca ggcgcatgac cgtcgtgaaa aaacaggtcc
     1681 ggcagcggga gaatgacgga ttcccggttt ctgatgttac ctttcaggca gaaaacgaaa
     1741 caggacgatg aaaattatct cgtacaacgt gaacggcata cgcgctgcgc tgggaaaagg
     1801 ctggatcgac tgggtgaaga aggaagatcc ggatgtgatc tgcctgcagg agatcaaagc
     1861 ggaaccgggc cagctcgatc tttccgtttt tgaaaaggcc ggttatgaaa atcatttctg
     1921 gcaccctgcg gaaaagaaag gctacagcgg cgtggctatt ctttcgaagg aaaaaccgga
     1981 taatgtgatc acaggctcgg gaaataaaac ctacgattcc gaaggaagga tcctgcgcgc
     2041 cgatttcggc gacctttccg tgatgagcct gtacatgcct tcgggaacga caggcgatgt
     2101 gcgccaggat ttcaaatacg gcttcatgga ttttttccag acgtacatcg acaagctgaa
     2161 aaaaacgcgg cccaacctcg tgatctcggg cgactggaac atctgccacc gggcgatcga
     2221 catccacaac ccggtgagca atgcgaattc atccggcttc ctgcctcctg aacgcgaatg
     2281 gataggccgg ttcatcgaca gcggcttcgt cgattccttc cgccatttcg agccggggcc
     2341 gcatcactac acctggtgga cattccgcgc caatgcaagg gcgagaaatc tcggctggcg
     2401 tatcgattac cacatggtga gccgcccgct ggaaaaaaag ctgaaagccg catccatact
     2461 gcccgaagcg aaacattccg atcattgtcc cattgttgtc gaactgaatt attagagcag
     2521 caggattaaa cccgccggtg tggccggcca tgccggacgg ccattcaccc tattttcact
     2581 tacgggtaac ctaattttca ttattttagg cgctgcatta tcataaacgc tgaatcccat
     2641 atgaaacgac tggtatttac actggctttt tcggcccttg cagcatctgt tgtgatcaac
     2701 ataaatggct gtggtccggg tgatgaagga aacggccagg aacgtaaagc gaatggcccc
     2761 gtagttcatt acggcggagt ttttcgtatg aatgaaacgg aagatttccg cacgctttat
     2821 cctctcaaca ttgtggaagt cgtgggtgac cacatcggtt cacaggtata cgaaggtctt
     2881 gtgaaattca accagaaaga cctgagcatc atgccgggac ttgccacgcg atgggatatc
     2941 aaagacagct ccacgcattt cattttccac atgcgtaagg gcgttttctt ccacgacgat
     3001 ccctgcttcc caggcggaaa aggacgcgag gtgaaagctt ccgacatcaa gtattgcttc
     3061 gatatgcttt gcacggcaag cccgaacaac cagcaatact ccaacacgtt caaagaccgc
     3121 gtggcgggtg cgaatgaata tttcgcttcc accgacagca aaaagcctct cgccggcggc
     3181 gtttcaggcg ttaccgttcc cgatgattcc accgtcgata tccgcctcgt gcgcccgatg
     3241 cccggtttcc tcaacattct cgccacgcag ggctgctgga tctatcccca ggaagcgctc
     3301 gccaagtacg gcgatgaaat gcgtgtgcat tgcgtgggaa caggtccttt ccgtgtgaaa
     3361 agcatcaagg aaggcgatgc ggttatcctg gaatacaacc cgaaatactg gatgttcgac
     3421 gaatggaaca accgtcttcc ctacctcgat atcgttgagt tccgtttcat caaggacaag
     3481 cgttccgaga tcatcgcgtt caagaacaaa gagctcgata tggtcttccg tcttcccgtg
     3541 gaaatggcga acgatatcat gggcgagctg agccaggcca aagagcatgg tgcggatttc
     3601 gttttccaga gcaatcctgc catgagcctc acgtattacg gtttccagaa caagctttct
     3661 ccgttcgaca acgtgaaagt gcgccaggca ttcaactatg cgatcaaccg cgagtacatc
     3721 tgcgaaaaag tgctcatggg tgatgcggtt ccggcggttt acgggatcgt tccacccagc
     3781 cttcccgggt acgacgccaa gacgatcaac gggtacaatt acgatcccga caaagcgaaa
     3841 cagctgctgg cagaagcagg ttatcccggc ggaaaaaatt tcccggccac cacgctatac
     3901 atcaacaacg gcggcgggga ccgcaatacg atgaccgccg attatgtgcg caaacagctg
     3961 aacgacgtgc tcggcatcaa tgtgacggtg gagctggttc cgttcgcgca gcaccttgaa
     4021 aacatcgaaa ccgggaaagc gctcttctgg cgcaccggct ggatcgccga ttatcccgat
     4081 cctgaaacgt tccttaccac gctttacgga aaacacattc cggctacgat gaacgaacgc
     4141 tcttcgctga actccgttcg ttattccagc ccgctgttcg attcgctttt cgaggccgct
     4201 gcgaatgagc ctgacgccgt caaacgcacc gacctttatc accgcgccga ccaggtggcg
     4261 atgtccgatg cggcgatcat gccgatctat tatgaaacca acgatcgtct cgtgcagaag
     4321 aacgtccgcg ggttcgacat caacccgatg gaataccgcg acatgacgcg cgtgtatttt
     4381 gcccctgacg aaaatgcaac cggtgcgaaa aaagacaccg cgcagaaata atattcccgg
     4441 aaaaacaacg aagcccgttc tgagaacggg ctttttttat gactttgaag aagacgccag
     4501 ctctttcatc agcaggcgac gtgccacggg gaggaacgtt tcttccacgg ggatctcgag
     4561 ccgtgtttcc gccgcgtaga cagcgggatt gggtatatcg ggcacagctt tcaccagctc
     4621 cgtttttatg aattcatagg tatgcgtgat gctgtatgaa tacgagccga agaacgatgt
     4681 atgcagcgcc cgccacgtgg aaccgggttc ttcgaaaagc ttcacgctcc aggaataaac
     4741 attcgttttt gaagccgcgt tgcggagaaa aagataaccg caggaaacat cgagcggaag
     4801 aagtccgacc ggctgcatct cgatcccgtc ttcgatgaaa tcgaacagct gcatgccttc
     4861 attcacatga ccggcaaaac gcggcaggga aaaatcgaga atggattcga tctcttccat
     4921 gaccttcccg tcgctgacca ccttctcgta gttcaggcga aaggcctccg catcaaaacc
     4981 cgtcagccgc tgcgggaaaa gcgaggcaag ctgctctttg ttttccttca gcgtttttgc
     5041 aaaacggtaa tgcgagatga ggtcggccag cggcggatac agcttcaccg cgcgaaaaca
     5101 ttcatccacg tgctggaggt aggccagcag caggtatttt ttgtattcga aatcgatgct
     5161 tttctctgtg atccagtctt tattcagctt ttccattttg ttgaggcact ttttttgtat
     5221 tataacgctg gttccgggca aagtgtttcg gacgataatt atcaattaac agttaataac
     5281 ttcaccccgc cgcgtcgatc gaaaggtccg gctggtttat ctttgaacga tacaaaacac
     5341 taattcatag ccacatgcgc atcaaaatat ccgcacttgc ccttcttgcc gcggtcgccc
     5401 ttaccacctg cgttccggca aggaagtacg acgaagaaaa acaaaaacgt gaagaagccg
     5461 agaagcagct cgccgatctt aaagcgcaga accaggatta cgaaacgaaa aacaaggagc
     5521 tcgaccagca gctggcagac agcaagaagc aggtcgcagg actgatcagc gacacgtcgg
     5581 gatgcggcat gcgctaccgg ctgcttacca gccagtacga tcagctgacg gccaattacg
     5641 agctgctgct caagcagaat aaagatctca tggcgcgcca gtcgaacgag aacacacagc
     5701 tcgtcggcaa gctgaacatg acgcaggagc agctgatcca gtaagaggtt tcgctgaagc
     5761 agctggatgc gagcctgcag cgccagaagc attcgctcga ttcgctgacc atcgagctgc
     5821 agaaacggga ggcgcgcgtg aaagagcttt ccactgcgct cgcacagaaa gattcggttg
     5881 tcaccgcgct gaaagcaaaa gtgaaggcgg cgctcgtcgg cttcgaagga aaagggctga
     5941 ccgttgaaga aaagaacggg aagatctatg tttcgatgga agaaaagctg ctgttccctt
     6001 cgggaagcta taaagtttcg caggacggga tgaatgcgct gaaatcgctg gcggacgtgc
     6061 tcatcgcgaa tcccgacatc aacatcatgg tggagggaca tacggatgac gttccctaca
     6121 agggaacagg cgacatcaag gacaactggg acctgagcgt attgcgcgcg acgtcggtgg
     6181 taaaaattct tgtcgccgac ggtgtcgatc cgaaacggct gaccgcggcg ggacgcggag
     6241 aatatttccc ggtcgatcct gcgaaaacgc ccgaggcccg tgcgaagaac aggcggacgg
     6301 aaattatcct tacgccgaag ctcgacgaaa ttttcaaagt gctcgacaac aactaacaga
     6361 aagaaattca ggcaggccac cttcacggtg gccttttctt ttattcaacc caatacgcat
     6421 cgcatgaaaa attccgttct ttcctgctgc attttcctcg ccgtcatctc tgcagcgaac
     6481 gcacaaacct attccggtaa gctcggttat ggtttttctc ctcccggctt ccagctcgat
     6541 ttttcacaga caggttcgtt cctgcaggaa gtcgccggct tctgcggagg cggcgtggtg
     6601 tatgccaaca ccgactggag gaacgattcc gcttcgggcg ggatcatccc gcagttgcag
     6661 aaagtagtcg cgcagctgca gccttcaccg ttcgcctaca ccgacatgct cacattcgcg
     6721 tgggccgatt atcccgcgat ccatcttgcc tcgccatcca acccggtgaa taactggacg
     6781 aatgcgcaga cgcgcgccga tttcatgacg atgctggtcc ataccgcaga ttcattgcag
     6841 ccggcgtaca tgttcattgg caatgaagtg agcttttaca tcgagcagga ttcgctcgac
     6901 tatctcaatt tcatcagctt ctatgaaagc acatacgatt ccatcaagct gcattcgccg
     6961 aatacgaaag tgggaacgat cttcaattac gagcacatca atggctacgg caacctgaac
     7021 gggtggacgg acaagcactg gtcggcgctg acgctgatcg actccacgaa actcgacgtg
     7081 atcggttttt cgctttaccc gttcctttca gttcccacgg cgaatgcaat gccggcggat
     7141 tacctcgaca cgatgtttgc gcgtacggga aacaagcccg tggtgataac ggaaaccggg
     7201 tggcccgcgg attcactgta cgggacatgg gtgagcagtc cgcagcagca ggtggattac
     7261 gtgaaccagc ttttcccgaa gctggcggga caaaatgtgg aagacgtgaa ctggctttac
     7321 ctgaattacc tgatcgacac cacatccaac gtcgggccgc tgatcttcaa aagcgtcagc
     7381 ctccgggatt ccctgggcaa cgaccggccg gcgcttccgt tgtgggaatc gatgtgtccg
     7441 ctgacgggaa tttcgcctgc gcagaatgaa aaaacgctgg ccttcttccc caatccttcc
     7501 gatggaaatt tcacggtgca ggatccgcag gcggcgaatg cacagcttcg catagatgac
     7561 gcggaagggc gggaagttta ttccggcatc ctcgccgcag gaacgaatcg cgtttcactt
     7621 tcacttcccg atggctttta cattgcgact gttttcaccg aaggtgaata tcccgtcacg
     7681 caaaagatcg tcatccgtca ctaattttac ggcatgaccg accgcagatc ttttatccgc
     7741 accatcggca tcgcttcagc ggcgatgtcg ctgccttcgt tcgcacgagg ccagtcgtcc
     7801 gctgttaaat cgcagaatgc aggcggcgca ctgaagccgg cccggctgaa acccggcgac
     7861 cttatcgcgt tcacggcacc ggccggcgcg atattcaatg acgattcggt acaaaaagcg
     7921 acagccgcgt tcgaagcgca gggattccgc gtgaagcacg gaaaaacact gtcggaaaaa
     7981 ttcggttacc tcgccggcaa agatgatctc cgcgcgaaag agctgcagga tcttttttcg
     8041 gataaggaaa cgaaagccat aatggcgatg cggggcggat ggggcagcgc gcgactgctt
     8101 ccgctgatcg acccgacgat catcgcagcg tcaccgaaaa ttttttccgg tttcagcgac
     8161 atcacgacat tgctcgcggc gttccaggtg acgaccgggc ttgtcacgtt ccatggtccc
     8221 gtaggaaatt catcactgga agatttcacg atgacgcatt ttctcgatct cgttgcgaat
     8281 gcagcgtcgc cggaactggt gcaggatgac gtgacaatac ttgcggaagg agaagcagaa
     8341 gggatgctgc tgggtggaaa tctcaccgtg ctctgcgcga tgatcggtac gccgtggatg
     8401 ccggagctga aggattctct tcttttcctc gaagaaacgg aggaagaacc ttattccatc
     8461 gaccgcatgc tgacacagct gacgcttgca ggtgtattca ccaaaacaaa aggcgtcgta
     8521 ttcgggcaat gcgtaaaatg cgtagcagaa gaacccgaga aatcgttcac gctcgacgag
     8581 gtgctgcgcc agaaactttc ttcgctcggc attcccgtga tgacaggatt ttcattcggg
     8641 cacatcaaag acaagttcac gctcccgctg ggcattcgcg cacgcatgag cacacagcgc
     8701 aaatcgcttc agctgctgga aagcagcgtg gcatgattac tgcggctttt cgtaactttc
     8761 ttttcccaaa aaccaaaacc catgaaacgc atttttactt tcctgcttcc cttattgttt
     8821 ctttccgctt cgttccgcgc acaaccggcg aacgacacgg tgatcttcac ccagctcggg
     8881 catttttatt acgacacagt cgtatggcgc gccgcttatt ctccgctgat cgaccggctc
     8941 gggcggccgt atgtgtatac cgcatccgtc gatctcggca tggtgacgtt cgacatcaac
     9001 gacccgatga acccggttcc tgtcgacacg atccggcctg cggatctcaa cggctataaa
     9061 gcgacttttc ttgcgcagga tgccgataca cttttcataa ccacgggcgg cttccagggt
     9121 ttcacgcaac gggccgggct ttccatttac gatgtaacca accctgccac cccggtgctg
     9181 atggaccact gggacaccgc agcgtgggcg cacggcgttt cccatattgt ccttgatgga
     9241 gattatgcat tcctttccgc gatgaacgat ggcatcatcg tgcttaacat atccgacaag
     9301 cacaacatcc gttttgtttc gcattttgtt ccgaccacta cgccgaacac atcgtacgcc
     9361 ccgcataccc gcggattgta tctcgacaac gatacgctca tcgatgctga cgatggcggc
     9421 ggactgcgca tcatcgatgc aacggataag cagaatcttg tgcagatcgg cgcgtacctg
     9481 aacccggtgc cgcacgccac ggacacggtt tatccttttt acaaccatgt gtggtgcatc
     9541 ggcaagcacg catggatccc tgttgattat gcaggaatag aggtcgacga ttattccaat
     9601 cctgcaagca tcacgaccga tgcatggtcg aatatctgga acaacaccgg tatcagctcc
     9661 tggtacggca gcgacggaca caccaacgag atcgtgtata ccggtccgac cacgaatgtg
     9721 ctcatggttt ccggcgccga ttcgcaggtg ctcgcatttg atccttcgga tccggccaac
     9781 ccgcgcatca tgggctggtg gggaccaccg aacacggaca acgaggccgc gtggggcgtg
     9841 gacgaattca acgggctcgt cgtgtgcggt tacctgcggg acgtcggttc gaaccagccg
     9901 tattacagca cgtatggcgg catacagctg ctcagctgga acctgctggt gaccggggag
     9961 aacgataatt ctcctgcaac ttccacgctg atgatctcgc ctaacccaac gaacgataaa
    10021 gcaacagttg cattgccggc ctcaacggac gagctgttca cgatcaacct cattgacgta
    10081 acgggccgcg tggtgcagac gcagagcgca gatccgaaaa tgaacggacg taatgcagag
    10141 atcgacatga cgaatcttgc acccggaatt tatacggtgt gtgtgcacgg caaaacgtcg
    10201 atgtggacgg ggaaagtggt gaaagagtga ttgtgccggg agatctgatg ctgaacggtt
    10261 ccgagtgcac ggcattgcac ctgtccggct gagcgcgagt cgaagcccgg caggtgccag
    10321 ttattcagtg tcaaaaaaac tgaacgcggg tgactgatca aaaacattgc acctgtccgg
    10381 ctgagcgcga gtcgaagccc ggcaggtgcc agttactcag tgtcaaaaaa actgaacgcg
    10441 ggtgactgat caaaaatatt gcacctgtcc ggctgagcgc gagtcgaagc cgggcaggtg
    10501 ccagttactc accaatcctt ctccacacat cgcggggaac atatcccggc tgatccagaa
    10561 gtcttttcga atttgtcgca ttccggcaaa cagcattcat cttcaacgcc tcccaatcgc
    10621 tgttgatcaa cgcttctttt tttactctcg accatttctt gatttttttc tcccatgaaa
    10681 tagcaaacca tgcattttga aatgcttccg cccaaacaag ttttaccggc cttcgggaaa
    10741 aggtgtaaga atc
//
LOCUS       DASTSF010000580         5904 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_772139,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000580 DASTSF010000000
VERSION     DASTSF010000580.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5904)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5904)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5904
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_772139"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..799)
                     /locus_tag="VFU15_16435"
     CDS             complement(<1..799)
                     /locus_tag="VFU15_16435"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEU4719435.1"
                     /translation="MKTLTGDNISMAAEFLRKGELVAIPTETVYGLAANALDADAVLK
                     IFETKGRPTFNPLIVHVHSAKEFRKYVKSVPRLVSTLAKEFSPGPLTFVLPKNEVIPD
                     IVTGGGDTVALRVPGHPLTLKLLSQLDFPLAAPSANPFGYISPVNAAHVLEQLEGKIP
                     YVLDGGPSMIGVESTVVTLEKNRLVVLRLGGVSVEDLKDVAGEVEVRTNQSSNPRSPG
                     QLKSHYAPSIPLRFGRMELLLKEYAQKKKALLTFSRQYIGKKIVAEEV"
     gene            840..1862
                     /locus_tag="VFU15_16440"
     CDS             840..1862
                     /locus_tag="VFU15_16440"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU4719436.1"
                     /translation="MERSPAPAVSVILPVFNAEKTVQRAIESVLTQTFHDFELLLADD
                     GSMDSTNIILEETAAKDHRVRVITLAHTGVAGAFNAGVKAANGKYVARMDADDEMLPQ
                     RLEKQFDFLEARPATGVVSCLVEYGGDRATQEGYSAHVDWINSIITHEQIMLNRFVES
                     PLCNPSVMFRKELFEKHGGCREGNFPEDYELWLRWLEAGVRMEKIRETLFRWNDLPGR
                     LIRNDERYSADNFFRIKEEYLSRFISKMIGGRKLFIWGAGKLSGKRSSFLAERFSSCG
                     YIDIDPKKAGGKKNGLPVIYHENIGAPGTIFVASFVTNRGKREEVKQFLEKRNFREGV
                     DFILAG"
     gene            complement(1871..2578)
                     /locus_tag="VFU15_16445"
     CDS             complement(1871..2578)
                     /locus_tag="VFU15_16445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719437.1"
                     /translation="MKYITLLVFFGFSFPARGKDVPTLRDLFLSLPDSVFIKDNRCFH
                     QKDSFPVVERKQLLAGFDSGNVENKGDGPRFPMFGLSDSSGILEFGTGDETTVLSILS
                     RDKKETFFLVASSDCDFIACFQSFHFYSLKKKKISEVNNVLPALYPAKLFFDTAYLAQ
                     QKFDPDQGLDGIEIVFTGDPHLLVASLNTDFFDPELFGYENAMTQLDASKIKWYSYRL
                     DFDPVKRKFVVGVCADE"
     gene            complement(2585..3232)
                     /locus_tag="VFU15_16450"
     CDS             complement(2585..3232)
                     /locus_tag="VFU15_16450"
                     /inference="COORDINATES: protein motif:HMM:NF024852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEU4719438.1"
                     /translation="MKKTAVVFGATGLTGRNLVRLLCEDERYDVVKIFTRKESGIKGA
                     KIDEIVIDFRKMDDNRKLVTGDEVFCCLGTTIAAAGSEAAFRRVDFEWVRWCAISAAE
                     NGVKKFLAISSLGANPASKNFYLRTKGEMEKAVSAVNFEKCAIFRPSMLLGARKEFRF
                     GELTGKFFMRAFSVFVPKKYKAVRSETVAKAMLKVANDAALNGVIGNELILALGK"
     gene            complement(3255..5888)
                     /locus_tag="VFU15_16455"
     CDS             complement(3255..5888)
                     /locus_tag="VFU15_16455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase/topoisomerase IV subunit A"
                     /protein_id="HEU4719439.1"
                     /translation="MSDKENNDIFENPEEGDNQQHEERSGVKIISGMYENWFLDYASY
                     VILERAVPYVEDGLKPVQRRLLHSMWEMEDGRYNKVANVIGHTMKYHPHGDASIGDAL
                     VQLGQKDLLIDTQGNWGNIFTGDSAAAPRYIEARLSKLALDIVFNPKTTTWQSSYDGR
                     NREPVTLPMKFPLLLAQGVEGIAVGLACKILPHNFNELIDASIAVLRGRKTDIMPDFP
                     TAGMADFSNYNDGLRGGKIRIRSRIKVKDSKTLVITEIPYGTTTTSLIDSVISANDKG
                     KIKIRKIEDNTAENVEILIHLQPGTSTDKTIDALYAFTACEVSISPNACIIEEEKPRF
                     VGVNEILRISALRTKDLLQKELEIELSELQEKWHFSSLEKIFIREEMYIDFKKYTDRE
                     SLYAYLYERFKPHRKKLLRPIDDNDLHRLTQIPMIRITRFDSIKADEQMKELENKIGG
                     VKHHLAHLTDYAIEYFKELKKKYGTGRERKTEIRQFEVIEAREVVMNNVKLYANREEG
                     FIGSGLKKDEFICDCSDLDEIIVFRGDGVMMVTKISDKTYVGKDIIHIAVFKKGDDRT
                     LYNMCYRDGLRGNTMIKRFHVTGVTRDKEYNLTRGAKDSKVHYFSVSPNGENEVITVF
                     LTPTSGARKREFDMDLSQVAVKGRDSQGNILTRYPVRKIVLKEKGEAEVVKQKVWFDD
                     TTHRLNYEERGTYLGSFGPDDRILTVTQSGHYRMTGIETTLHFDEDMIVIEKYDPKAV
                     TTCIYMDGESGQYFVKRFTFDLTDKKTLFITENPGSRVEAISNHPSQKIELKFVKVKG
                     VEPPPETIEADEFIEVRNMKAKGNKLSANKIKEVDMVDPFAEISDEDIEMESSGDSPL
                     QKMRKEKGKKGKGGRPQMELF"
BASE COUNT         1266 a         1616 c         1545 g         1477 t
ORIGIN      
        1 gcacttcttc cgccacgatc tttttaccaa tgtactgccg cgaaaacgtc agcagcgcct
       61 ttttcttctg cgcgtattct ttcagcagca gctccattct tccgaagcga agcggtatgg
      121 aaggcgcata atgcgatttc agttgaccgg gcgaccgcgg gttagacgat tggttcgtac
      181 gcacttccac ttctcccgcc acatccttca ggtcttccac ggaaacgccg ccaagacgca
      241 gcacgacgag cctgtttttt tccagcgtca ccactgtcga ttccacgccg atcatgctcg
      301 ggcctccgtc gagcacgtac ggaatttttc cttcgagctg ctccagcaca tgcgccgcat
      361 tcaccgggct gatgtaaccg aaaggatttg cgctcggcgc cgccagcggg aaatcgagct
      421 gcgaaagcag tttcagcgta agcggatgtc ccggcacacg cagcgctacc gtatccccgc
      481 caccggtgac gatgtcggga atgacctcat ttttcggcag cacgaacgtc agcggtcccg
      541 gcgaaaattc ttttgccagc gtcgacacaa gacgcggaac cgacttcacg tatttgcgga
      601 attccttcgc gctgtgcaca tgcacgatga gcgggttgaa cgtgggacgt cctttcgttt
      661 cgaaaatctt cagcaccgca tccgcatcga gcgcgttggc ggcaagaccg taaacggttt
      721 ccgtcgggat ggcgacgagc tctcctttgc gcaggaattc ggccgccatt gaaatgttgt
      781 cgccggtgag ggttttcatt cgaaggtcaa agatacctta acttgcagaa atgattcccg
      841 tggaaagaag ccccgctcct gccgtttccg tcatcctccc ggttttcaat gcagaaaaaa
      901 ccgttcagcg cgccattgaa agtgtgctga cgcagacgtt tcacgatttc gaactgctgc
      961 ttgctgacga cggttcaatg gattccacga atatcatcct ggaagaaacg gcggcaaaag
     1021 atcatcgcgt acgcgtgatc acattggcgc acacgggcgt ggcaggcgca ttcaacgcgg
     1081 gtgtgaaagc cgcgaacggc aaatacgtcg cacgcatgga cgcagacgac gagatgcttc
     1141 cgcaacggct cgaaaaacaa ttcgattttc tcgaagcacg cccggcgacc ggtgttgtca
     1201 gctgtctcgt ggaatacggc ggcgaccgtg ctacgcagga aggctattcc gcccacgtcg
     1261 actggatcaa ttcgataatt acgcatgaac agatcatgct caatcgcttc gtggaatcac
     1321 cgctgtgcaa tccgtccgtc atgttccgca aagagctttt tgaaaaacac ggcggctgcc
     1381 gcgaaggaaa tttccccgag gattacgagc tctggcttcg ctggctggaa gcaggtgtgc
     1441 gcatggaaaa aatccgggaa actcttttcc gctggaatga cctgccggga cgtctcatcc
     1501 ggaacgatga acgctattcc gccgacaatt tctttcgcat caaggaagaa tatctttcgc
     1561 gtttcatcag caaaatgatc ggcggcagaa agctgttcat ctggggggcg ggaaagcttt
     1621 cgggaaaacg aagcagtttc ctggcggaac ggttttcttc ctgcggctat atcgatatcg
     1681 atccgaaaaa agccggcgga aagaaaaacg gcctgcccgt tatttaccat gaaaacattg
     1741 gcgcaccggg cacaattttc gtcgccagct tcgtcaccaa ccgcgggaag cgtgaagaag
     1801 taaaacagtt cctcgaaaaa aggaacttcc gggaaggcgt ggattttatt cttgcgggct
     1861 gaacgaatag ttattcatcc gcgcagaccc cgacgacaaa tttccgtttc accgggtcaa
     1921 aatccaggcg ataggaatac catttgatct tcgacgcgtc gagctgcgtc atcgcgtttt
     1981 catatccgaa aagttccggg tcgaaaaaat ccgtgttcag cgacgccaca agcaggtgcg
     2041 gatccccggt gaaaacgatc tctatcccgt cgagtccctg gtccgggtcg aatttctgct
     2101 gtgccaggta agcagtatcg aaaaaaagct ttgccgggta aagcgcgggc agcacgttgt
     2161 tcacttccga aatctttttc tttttcaacg aataaaaatg gaacgactgg aagcaggcga
     2221 tgaaatcgca atcggatgaa gcgaccagga agaacgtctc tttcttatcg cgtgacaaaa
     2281 tggaaagcac agtggtttca tcacctgttc cgaactcgag gatgccggaa ctgtcggaaa
     2341 gaccgaacat aggaaaacgc ggaccgtcgc ccttgttttc cacgttgccc gaatcgaaac
     2401 cggcgagcag ctgtttccgt tcgacaaccg ggaaggaatc tttttgatgg aaacaacgat
     2461 tgtctttgat aaaaacggaa tccgggagcg agaggaaaag atcgcgcagc gtgggaacgt
     2521 cttttccccg ggcgggaaat gaaaaaccga agaaaaccag gagggtgatg tatttcatgc
     2581 cgcgttattt acctaaagcg agaatgagct cattcccgat cacgccgttc agcgccgcat
     2641 cgttggccac tttcagcatg gccttcgcca cggtttccga acgtacggct ttgtatttct
     2701 tcggaacgaa aacagaaaat gcacgcatga aaaattttcc ggtcaattca ccaaagcgaa
     2761 attccttccg cgcgccgagc agcatcgaag gacggaagat cgcgcacttt tcaaaattca
     2821 cagcgctcac cgctttttcc atctcgccct tggtgcgcag gtaaaaattc ttcgaagcgg
     2881 gattggcgcc cagcgatgaa atggcgagga atttcttcac cccgttctcc gccgcgctga
     2941 tcgcgcacca gcgcacccat tcgaaatcca cgcgacggaa agcggcttct gaaccggcag
     3001 cggcgattgt cgtgccgagg cagcagaata cttcgtcgcc cgtaacgagc ttcctgttgt
     3061 catccatttt ccggaagtcg atcacgatct cgtcgatctt cgcccctttt atccccgatt
     3121 ctttccgtgt aaagatcttg accacgtcat aacgctcgtc ttcacacaaa agcctgacga
     3181 ggtttcttcc cgtcaggccc gtggcgccga atacgaccgc tgttttcttc atgaagtcag
     3241 ggtatgtgcc atgctcagaa tagttccatc tgcggccggc cgccttttcc ttttttccct
     3301 ttttccttgc gcatcttctg cagcgggctg tcgccggacg attccatctc gatgtcttcg
     3361 tctgaaatct cagcgaaagg atcgaccatg tcaacttcct tgatcttgtt agcgctgagc
     3421 ttgttccctt tcgctttcat gttgcgcacc tcgatgaact catccgcttc gatcgtttcc
     3481 ggcggaggtt cgacaccttt cacttttaca aatttcagct cgatcttctg cgaaggatgg
     3541 ttcgagatcg cctccacgcg cgaaccggga ttttcagtga tgaacagcgt tttcttatcg
     3601 gtaaggtcga acgtgaaacg cttgacgaaa tactgcccgc tctccccgtc catgtagatg
     3661 caggtggtta ccgctttcgg atcgtatttc tcaatgacga tcatgtcttc gtcgaaatgc
     3721 agcgtggtct cgatgcccgt catgcggtaa tgacccgatt gtgtaaccgt gagaatgcga
     3781 tcgtccggac cgaaagagcc gaggtatgtt ccgcgctcct cgtagttcag gcggtgtgtc
     3841 gtgtcatcga accatacttt ctgtttcacg acttcggctt cgcctttttc tttcagcacg
     3901 atcttgcgta cggggtaacg cgtgagaata tttccctgcg aatcgcgtcc tttcaccgcc
     3961 acctgcgaaa ggtccatgtc gaattcccgc tttcgcgcgc cggaggtcgg tgtgaggaaa
     4021 accgtgatga cctcgttctc cccgttcggg ctgacggaaa aataatgcac tttggaatct
     4081 ttcgctccgc gcgtgaggtt gtattccttg tcgcgggtga cgcctgttac gtggaagcgc
     4141 ttgatcatgg tgtttccgcg caggccgtcg cggtagcaca tgttgtagag cgtgcggtcg
     4201 tctccttttt tgaaaacggc gatgtggatg atgtctttcc ctacgtatgt tttgtcggat
     4261 attttcgtca ccatcatcac gccgtcgccc cggaaaacga tgatctcgtc caggtcggag
     4321 cagtcgcaga tgaattcatc cttcttcagc ccggaaccga tgaaaccttc ttcgcggttg
     4381 gcgtacagct tcacgttgtt catcaccact tcccgcgctt cgatcacttc gaactggcgg
     4441 atctcggttt tgcgctcgcg tcccgtaccg tatttctttt tcagctcttt gaaatattcg
     4501 atcgcgtaat cggtgagatg cgccaggtga tgcttcactc ccccgatctt attctcgagc
     4561 tccttcatct gctcatccgc cttgatgctg tcgaaacgcg tgatgcggat catcgggatc
     4621 tgcgtcaggc ggtgcaggtc gttatcgtcg atcgggcgaa gcagcttttt gcggtggggc
     4681 ttgaaacgct cgtacaggta tgcgtacagg ctttcgcgat cggtgtactt tttgaaatcg
     4741 atgtacatct cctcgcggat gaatattttt tccagcgaag agaagtgcca tttttcctgc
     4801 agctccgaca gttcgatctc cagctctttc tgcagcaggt ctttcgtgcg cagcgcggaa
     4861 atcctcagta tttcgttcac accgacgaag cgcggcttct cctcctcgat gatgcacgca
     4921 ttcggggaaa tggaaacttc gcatgcggtg aacgcataca gcgcatcgat ggttttatcc
     4981 gtggacgtcc cgggctggag atgaatgagt atctcgacgt tttccgccgt gttgtcctcg
     5041 atcttgcgga tcttgatctt tcctttgtca ttggcggaaa tgaccgaatc gatgaggctg
     5101 gtggtcgtgg ttccgtaagg aatttcggtg atgacaagcg ttttgctgtc cttcaccttg
     5161 atgcgggagc ggatacggat ctttccgccg cgaaggccgt cgttgtaatt ggaaaaatct
     5221 gccattccgg ccgtcgggaa atccggcatg atgtccgttt tccttccgcg cagcaccgca
     5281 atggaggcgt cgatgagctc gttgaaattg tgcggaagta ttttgcaggc aaggcccacc
     5341 gcgatgcctt ccacgccctg cgcgagcagc agcgggaatt tcatcggcag tgttaccggc
     5401 tcgcggttac ggccgtcgta gctcgactgc caggtggttg ttttgggatt gaaaacgata
     5461 tcgagcgcca gctttgaaag ccgcgcttcg atgtaacggg gcgcagctgc gctgtctcct
     5521 gtaaaaatat ttccccagtt accctgcgtg tcaatgagca ggtctttctg cccgagctgc
     5581 acgagcgcat cgccgatgga agcgtcgccg tgcggatggt atttcatcgt gtgcccgatg
     5641 acgttcgcca ctttgttgta acggccgtcc tccatctccc acatggaatg caggagacgg
     5701 cgctgtaccg gcttgaggcc atcctcaaca tacggcacgg cgcgttccag gatcacgtaa
     5761 gaagcgtaat cgaggaacca gttctcgtac atcccggaaa tgatcttcac gcccgaacgc
     5821 tcttcatgct gctggttgtc tccttcttcc gggttttcga agatgtcgtt gttctctttg
     5881 tctgacattc ttaacttgaa ttag
//
LOCUS       DASTSF010000581         2524 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1679216,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000581 DASTSF010000000
VERSION     DASTSF010000581.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2524
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1679216"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..314)
                     /locus_tag="VFU15_16460"
     CDS             complement(<1..314)
                     /locus_tag="VFU15_16460"
                     /inference="COORDINATES: protein motif:HMM:NF014409.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HEU4719440.1"
                     /translation="MKRLIFIQARMGSTRLPGKVLKPLAGKPQLQHLVDRLGKYSGAG
                     EVVVVTSDLPADDAIEKFCGEQEVKCFRGSEWDVLDRFYAAAKAFHAAPGTVIVRITA
                     DCP"
     gene            complement(311..847)
                     /locus_tag="VFU15_16465"
     CDS             complement(311..847)
                     /locus_tag="VFU15_16465"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEU4719441.1"
                     /translation="MAFFLETDRTGLRPLATEDATGEYLGWLNDHEVTRGLETGIFPS
                     TKESLVDFVKRMGSSKDNLIFAIIDRQSGKHIGNIKLGNINWVHRNGELGILIGDKSS
                     WGKGHGTDACRLLVQYAFEKLNLHKVWLAVFSNNPAAISIYKKLGFTEEGRLKEHVFS
                     EGKFVDKILMSVFNNAEK"
     gene            complement(847..1497)
                     /locus_tag="VFU15_16470"
     CDS             complement(847..1497)
                     /locus_tag="VFU15_16470"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004498743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HEU4719442.1"
                     /translation="MLVTVIITAHNYARYIERAIRSVQNQSLPREQYEIIVIDDCSTD
                     ETPQVLENYKDEVRVFRMEKNVGLAEARNFGIKKAKGQYVLFVDADDYIQYDLLKVQS
                     VFLTENNRLDAVAVDYYLVNEFGDHLEWVSAQEKPIACGVMFRKDRLFDVGLYDTSFR
                     AREDEDMRIRFLKKFNIYNIILPLYRYRRHNSNLTNNAEEMEKYRQKLDEKHNGNS"
     gene            complement(1518..>2524)
                     /locus_tag="VFU15_16475"
     CDS             complement(1518..>2524)
                     /locus_tag="VFU15_16475"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU4719443.1"
                     /translation="AWLYEFTRNPHRKYLLRHFRKNTGVLFYQLISASVFGRILERYS
                     GDELYFYTYWFHHATLMLAFLKKQDKIRSFVSRAHSIDLYNEHWLYAGDRHKPLPYAW
                     FKMKYIRKVAAISEHGKEHLLKEFPDCREKISRSYLGVPDNGPGVSLPLQPFVIASCS
                     NFTGNKRVWLIPEILGGLGKDIRWVHFGSGGNTESQVKKAAEKFLSPEQVIFAGHVPT
                     AAIMEYYRTHPVHLFMNMSRAEGLPVSIMEAISFGIPVIATDVYGTPELLSGGNGFLV
                     SADDPVAKIAGLVDQLRNDAQLHKKMGEASRKLFLEKFSAGKTYPDFIRKVLHGLSDN
                     "
BASE COUNT          564 a          674 c          634 g          652 t
ORIGIN      
        1 gggcaatcgg ctgtaatgcg gacgatcact gtcccgggag ccgcatgaaa tgctttcgca
       61 gcagcataaa aacgatcgag cacgtcccat tcgctgccgc ggaaacattt cacttcctgc
      121 tcaccgcaga atttttcaat cgcgtcgtcg gcagggaggt cgctggtcac caccaccact
      181 tcgccggcac cggaatattt tcccagccgg tcgacgaggt gttgcagctg cggttttccc
      241 gccagcggct tcagcacttt cccgggaagc cgtgtagacc ccatccgcgc ctgtataaag
      301 atcagccgct tcatttttcc gcgttattga aaaccgacat gagtatcttg tccacgaact
      361 ttccttcgga aaagacgtgc tccttcaggc gtccttcctc cgtaaacccg agctttttat
      421 aaatggaaat ggcagccggg ttattggaaa aaacggcaag ccagactttg tgcaggttca
      481 gcttttcaaa tgcgtactga acaagcagcc ggcaggcgtc ggtcccgtgt ccttttcccc
      541 acgaagattt atcaccgatg aggataccga gctcaccgtt gcggtgcacc cagttgatat
      601 tgcccagctt gatgttgccg atgtgcttcc cgctctgcct gtcgatgatc gcaaaaatga
      661 gattgtcttt gctgcttccc atccgtttca caaaatccac gagcgattct ttcgtcgagg
      721 gaaagatgcc tgtttcgagg ccgcgcgtca cttcatgatc gttgagccat cccaggtact
      781 ctccggtagc atcttccgtc gcgagcggcc ggagtccggt acggtctgtt tcaagaaaaa
      841 aggccatcag gaattaccgt tatgcttttc atccagcttc tgcctgtact tctccatttc
      901 ttccgcgtta ttggtgaggt tgctgttgtg cctgcggtac ctgtacagcg gcaggatgat
      961 attgtagatg ttgaattttt tcaggaaacg gatgcgcata tcttcatctt cgcgggccct
     1021 gaaggaagta tcgtaaaggc cgacatcgaa aagccggtct ttccggaaca tgacaccgca
     1081 ggcaatgggt ttttcctgcg ctgaaaccca ttcgaggtgg tcgccgaact cattcacgag
     1141 gtaataatcg acggcaaccg catcgaggcg attgttttcc gtgagaaaaa cagattgaac
     1201 tttcagcagg tcgtattgaa tataatcgtc agcatccaca aaaagcacat attgtccttt
     1261 tgctttttta attccgaaat tcctggcctc tgcaagtccc acgttttttt ccatcctgaa
     1321 tacccgcact tcgtccttgt aattctccag cacctgcggc gtttcatcgg tgctgcaatc
     1381 gtcaatgacg atgatctcgt attgttcgcg cggcagcgac tggttctgca cgctccgtat
     1441 ggccctctcg atgtagcggg cgtaattatg ggcggtaatg atgacggtga cgagcataaa
     1501 tgcttcctaa attagaacta attatccgat aagccgtgaa gcactttacg tataaaatcc
     1561 ggataggttt tccctgctga aaatttttcg aggaaaagct tccgcgatgc ttctcccatt
     1621 tttttatgca gctgcgcgtc gttcctgagc tggtccacga gaccggcaat tttcgcgacc
     1681 gggtcatcgg cggaaaccag gaaaccgttt ccgccggaaa ggagttccgg ggttccgtat
     1741 acatccgtgg cgataacagg tatgccgaag ctgatcgctt ccatgatcga aactggcaga
     1801 ccttcggcgc ggctcatgtt catgaacagg tgtacgggat gcgttcggta atattccata
     1861 atggcggccg tgggtacatg gccggcgaaa atcacctgct ccggtgaaag gaatttttca
     1921 gccgctttct tcacctggct ttccgtattt cccccgctgc cgaaatgtac ccagcggata
     1981 tcttttccca gtcctccaag gatctcaggg atcagccaga cgcgtttgtt cccggtgaaa
     2041 tttgaacagg aagcgatcac aaacggttga agcggaagcg aaacgcccgg accgttatcc
     2101 ggcaccccga ggtagctccg cgatattttt tcacggcagt cgggaaattc tttcagcagg
     2161 tgctcttttc catgctcgga aatggccgct actttcctga tgtatttcat cttgaaccag
     2221 gcataaggca gcggcttgtg gcggtcgccc gcatacagcc agtgctcgtt gtacaggtcg
     2281 atcgaatgcg cgcgggaaac gaatgaccgt attttatcct gcttcttcag gaacgcgagc
     2341 atcaatgtgg cgtgatgaaa ccagtaggtg tagaaataaa gctcgtcgcc tgaataacgt
     2401 tccaggatac ggccaaacac cgacgcgctg atcagctggt agaacagcac tcccgtgttt
     2461 ttcctgaaat gccgcagaag gtatttccgg tgcgggttcc tcgtaaattc ataaagccat
     2521 gccg
//
LOCUS       DASTSF010000582         4397 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1729442,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000582 DASTSF010000000
VERSION     DASTSF010000582.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4397
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1729442"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            168..2462
                     /locus_tag="VFU15_16480"
     CDS             168..2462
                     /locus_tag="VFU15_16480"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEU4719444.1"
                     /translation="MRNFLRGVFFSLLVFPPFFVRGQIEQDTAMHAFAPDFTTFDQRT
                     PVVTDEMTHLTPSAYQANPDYGILPDNAPCTDCIELIQKRTLNSRYYIQNGTGGNVFY
                     VQTSYGNFNYVDSAGWIRAIDPKLYPVGGGIYKADIQPVPATLNMNDGTTTMSTDGLS
                     LVFNTNTSVYHVTDTSATLLSGAADLYSSTVGKNGAYTTNAWPGIDRQLVFNFNSVET
                     DYILNTAPNTTSQTGWYGFNDEITLPPGYVLFEDTISGGIRTPEGFWQGSLVVINTSD
                     NAEEARWAPVVVYDNIGNRAVISNGAYQVIQNGNTYTIRTLVKESWLTSSSTVYPVTV
                     DPLVSGAATYSAGNIGFSIYAPGDGFCGSSSAYCYGGPLNVTFPGQATATNAIWGLTY
                     RAFAPAWRSDGGFRMVGPCGEDPANTDNWYSCAVNSAGNCVGSGFNAPWLVTCLTPSC
                     VATTVPFHVKNIDCFGWAGPCSTAVLQTTNNTWNVTVQGHTTETLGNSASGNGSTTTT
                     GTCCTATTLNPSPLYGVPAYTYSWSTGATSPTISVSSCTNGSTVYTCTVTDACGISRT
                     ATITFSVSSCVLPVELTSFTGEFQPGLNSVLLNWNVASEHNNNYYLVERTTDGIHFDA
                     VGRVFSKAPGGNSSEPLDYSLTDPSPPSGVVYYKLSQVDISGNEQTEGYIAVSVSADG
                     MPLQIVPNPANDNALVTYFSPAEAESEIRLVDVTGRVLFSETQASKAGLNSFDIPVKG
                     CTSGVYFVRVTSGSSAQVARLIIQ"
     gene            2620..2826
                     /locus_tag="VFU15_16485"
     CDS             2620..2826
                     /locus_tag="VFU15_16485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719445.1"
                     /translation="METLKRSGIVAVFALFILSAMWWAAGFTPSPPQADETVNTANTS
                     RGSSREELKPQMDLKSIKMNNRHR"
     gene            complement(2845..3858)
                     /gene="hemH"
                     /locus_tag="VFU15_16490"
     CDS             complement(2845..3858)
                     /gene="hemH"
                     /locus_tag="VFU15_16490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018341847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEU4719446.1"
                     /translation="MKTGILLINLGTPDSPSTRDVRKYLREFLMDPEVIDINPVARWL
                     LVNLIIAPFRAPKSAKLYEKIWTKEGSPLLIYGNALTQKLQHSLGEDYVVKLAMRYRN
                     PSIRNVLREMMKENVEKIIAVPLYPQYASSSTGSTITRLEKEIAAQKITVPVKIISRF
                     HQRREYIDAVAAGAGSFDLSQYDHVLFSFHGVPERHITKTDAKLGNGKCYFGNCCDTI
                     HAGNEFCYRANCLATAHDVARKLSLSPEKYTISFQSRLGRTPWIRPYSDEIIAELAQK
                     GKKKILAFSPAFVADCLETIYEIGVEYDELFRRNGGEKVQLVPSLNADDSWVAALQKI
                     ILS"
     gene            complement(3959..>4397)
                     /locus_tag="VFU15_16495"
     CDS             complement(3959..>4397)
                     /locus_tag="VFU15_16495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719447.1"
                     /translation="IGAKGYTNGDLALRKIAQMVDTLHISGRCHVIMATDGIFHLSEK
                     TKAFADTVFAQRKTSFCVLQFGDEQNEDLKEVATISPDGMYALATRKNIPAILRRQVP
                     VPQKTAVPQTVYYTDIVKMRGAEFVQYYLQLNPDDGLASIPRR"
BASE COUNT          979 a         1175 c         1197 g         1046 t
ORIGIN      
        1 cgcagtgtga ttgccttacc ggtttttcct ccggaacaga tcacgctgct tattaggtga
       61 ttctaatttt tccgattgca ggcaaggtgg aattgttata aatttaactg gcaaaaaaac
      121 atgatttccg gcaaacccgg aagagagcac acaaaatgaa aacagcgatg aggaattttt
      181 tacgcggtgt ttttttcagc ctgttggtat tcccgccttt tttcgtgagg ggacagatcg
      241 agcaggatac ggcgatgcat gcgttcgctc cggacttcac cacgttcgac cagcggacac
      301 cagttgtgac ggatgagatg acccatctta cgccttcggc ttaccaggct aatcccgact
      361 atggcatttt accggataac gcgccctgca cggattgcat cgagcttatc cagaaaagaa
      421 cgctcaactc gcgctattat atccagaacg gaacaggcgg aaatgttttt tatgtgcaga
      481 ccagctacgg caatttcaat tacgtggatt ccgcgggatg gatccgcgcc atcgatccga
      541 aactgtaccc ggtgggcggt ggaatttaca aggcggatat tcagccggtc cctgccacat
      601 tgaacatgaa tgacggaact acgacgatgt caactgacgg actttcgctc gtgttcaaca
      661 ccaacacatc cgtgtatcac gtgacggata cttcagcaac gcttctttca ggtgcggccg
      721 atctgtattc ttccaccgtc gggaaaaacg gagcgtacac caccaatgca tggcccggca
      781 tagaccgcca gcttgttttt aattttaaca gcgtcgaaac cgattacatt ctcaatacgg
      841 caccgaacac aacgtctcag acaggctggt acggtttcaa tgacgagatc acgctgccgc
      901 cgggatatgt gctgttcgag gatacgatca gcgggggaat aaggacgccg gaaggattct
      961 ggcagggttc gctcgtggtc atcaacacat ccgacaacgc ggaagaagcg cggtgggcgc
     1021 cggtagtggt ttatgacaac atcggcaaca gggccgtgat ttccaacggc gcttaccagg
     1081 tgatacagaa cgggaacacc tacacgatcc gcacactcgt gaaggaaagc tggctgacga
     1141 gctcttcgac ggtttacccg gtgacggtcg atccgctggt gagcggcgcg gcaacatatt
     1201 cggccggcaa catcggtttt tcaatttatg cgccgggcga tggattctgc ggaagctctt
     1261 ctgcgtactg ttacggcggt ccgctgaacg ttacattccc cgggcaggcg acagccacca
     1321 atgcgatctg gggacttacc tatcgcgctt ttgcacctgc atggcgcagc gacggaggct
     1381 tccgcatggt aggaccctgc ggggaagatc cggccaacac cgacaactgg tattcctgcg
     1441 ccgtgaacag cgcgggtaat tgcgttggtt ccgggttcaa tgctccctgg ctggttacat
     1501 gtcttacacc gagctgcgta gccactacgg tgcccttcca cgtaaagaac atagactgtt
     1561 tcggatgggc aggaccgtgt tcgacagcag tgctgcagac gacaaacaat acgtggaacg
     1621 tgaccgtgca gggacatacc accgaaacgc tcgggaattc cgcaagcggg aacgggtcta
     1681 caacaacaac cggaacctgc tgcacggcca ccacgctcaa tccttcgccg ctgtacggag
     1741 ttcctgccta cacttattcc tggagcacag gcgccacctc gccgaccatc agtgtctcga
     1801 gctgcacgaa cggaagcacg gtttacacct gcacggttac ggacgcctgc ggaatttccc
     1861 gcactgctac cattacattt tccgtatcga gctgcgtgct gccggttgag ctgacttcct
     1921 tcaccgggga attccagccc gggctgaaca gcgtcctgct caactggaat gttgcatcgg
     1981 aacacaacaa caattattac ctcgtcgagc ggacaacgga tggcattcat ttcgacgcgg
     2041 tcggccgggt gttttccaaa gcgcccggcg ggaacagttc cgaaccgctg gattattcgc
     2101 tcacagatcc gtcgccgcca tcgggtgttg tttattacaa gctcagccag gtggatattt
     2161 ccggtaacga gcagacggaa ggttatatcg ccgtttccgt gagcgcggac ggcatgccgc
     2221 tgcagatcgt tcccaacccg gcgaacgaca acgcgctggt gacctatttt tcgccggcgg
     2281 aagcagaaag tgaaatacgt cttgtggacg tgacgggccg tgtgctgttt tcagaaacgc
     2341 aggcatcgaa agcaggtctc aactctttcg atattccggt caaaggatgc accagcggcg
     2401 tgtattttgt ccgcgtcaca tcgggaagca gtgcgcaggt tgcgcggctg atcatccagt
     2461 aaagattatt tttccattag attttatgaa aatttctgtt gtgtccggga gaacagaaac
     2521 tattcgtcga tgcgtttctg tgtttggcag aaatacgaaa ccggtttctg tttctattcg
     2581 tatatttatt cagcaaacac aacagaccct ttttcattta tggagaccct taagcgcagc
     2641 ggaatcgttg ctgtattcgc gctttttatt ttatcagcta tgtggtgggc ggcaggcttt
     2701 acaccatctc cgccgcaggc tgatgaaacg gtgaatacgg cgaacacttc gcgcggctct
     2761 tcccgggagg aactgaagcc gcagatggac ctgaaaagca tcaagatgaa taaccggcac
     2821 cgctgatcat cgcgatgcca ttggtcacga aaggatgatc ttctgaagtg ccgccaccca
     2881 gctgtcatcc gcattgagcg aaggtacgag ctgcaccttt tctcccccgt tcctgcggaa
     2941 cagctcatcg tattccaccc cgatctcgta aatcgtttcg aggcagtcgg ccacgaacgc
     3001 aggtgaaaag gcaagtatct ttttctttcc tttttgcgca agctccgcaa tgatctcatc
     3061 ggaataaggc cgtatccacg gtgttcttcc caggcgcgac tggaagctga tcgtatattt
     3121 ttcaggagac agcgaaagtt ttcgggcgac atcatgcgca gttgcaagac aattcgcgcg
     3181 gtagcagaat tcattccccg catgtatcgt gtcgcagcaa ttcccgaaat agcattttcc
     3241 gttgccgagc tttgcgtcgg ttttcgtgat atgccgttca ggcacgccgt gaaagctgaa
     3301 cagtacatga tcgtattgcg aaagatcgaa cgagcctgca ccggccgcca ccgcatcgat
     3361 gtattccctg cgctggtgaa agcgcgaaat gatcttaacc gggacggtta ttttctgcgc
     3421 ggcgatttct ttttcgagcc ttgtgatcgt ggagcccgtc gacgaagaag cgtattgagg
     3481 gtagagcggc accgcgatga tcttctccac gttttccttc atcatttcgc ggagcacgtt
     3541 gcggatggac gggttgcggt agcgcatggc aagtttcacc acgtagtctt ctccgagcga
     3601 atgctgcagc ttttgcgtga gcgcattacc gtatatcagc agcggacttc cttccttcgt
     3661 ccagatcttt tcatacagct ttgcggactt cggcgcacgg aatggcgcga tgatgagatt
     3721 gaccagcagc caccttgcaa ccgggttgat gtcgatcact tcgggatcca tcaggaattc
     3781 gcgcaggtat ttccgcacgt cccgcgtgga aggactgtca ggtgtgccga gattgatgag
     3841 aagaatgccg gttttcactg cgcaaagata aaagacacat cattgcgcag gcagccagga
     3901 cggacagttt tgcgatccgc tgatgcggcg gacagggcgt ggatgagaac acgcatattc
     3961 acctgcgcgg gatggacgca aggccgtcgt ccgggttcag ctgcaggtaa tactgcacga
     4021 attccgcccc gcgcattttc acgatgtcgg tgtaataaac ggtttgcgga acggccgttt
     4081 tctgcggtac ggggacctgc cggcggagga tcgccgggat atttttccgc gtcgcgagcg
     4141 cgtacatgcc gtcgggcgag atcgtcgcca cttctttcag gtcttcattc tgttcgtcgc
     4201 cgaactgcag cacgcagaag ctggtttttc tctgtgcgaa gacggtgtct gcgaatgcct
     4261 ttgttttttc gctgaggtga aaaatgccgt ccgttgccat gatcacgtgg cagcgcccgc
     4321 tgatgtggag cgtgtcgacc atctgagcga tctttctcag cgcgagatcg ccgttcgtat
     4381 atcctttcgc cccgatc
//
LOCUS       DASTSF010000583         3756 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_786836,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000583 DASTSF010000000
VERSION     DASTSF010000583.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3756)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3756)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3756
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_786836"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="VFU15_16500"
     CDS             complement(<1..287)
                     /locus_tag="VFU15_16500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012930119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein"
                     /protein_id="HEU4719448.1"
                     /translation="MLPSDQIDIRNRKASFEYAFLEKFIAGLVLTGTEIKSLRQGKAN
                     ITDGFCVFERGELFLRNVQISEYEKGTHYNHEPKRDRKLLLSKHELKKLQG"
     gene            complement(360..445)
                     /locus_tag="VFU15_16505"
     tRNA            complement(360..445)
                     /locus_tag="VFU15_16505"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(409..411),aa:Leu,seq:cag)
     gene            complement(534..615)
                     /locus_tag="VFU15_16510"
     tRNA            complement(534..615)
                     /locus_tag="VFU15_16510"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(579..581),aa:Leu,seq:gag)
     gene            829..1338
                     /locus_tag="VFU15_16515"
     CDS             829..1338
                     /locus_tag="VFU15_16515"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEU4719449.1"
                     /translation="MRKTILVVFLLVAGMKVDAAKYFIFPDTTGTPSVCDSIVTYATS
                     FLGTPYHYGCSSPKAGFDCSGFTWYVFGHYGIDIPRSAQAYATVGKEVSLSECRKGDI
                     ILFRGTHPGEKRVGHVGIIISGKGEPVKFIHASSSDNHRGVVITDYYSSHYPARFIKV
                     IRIIPEEKK"
     gene            1454..3604
                     /locus_tag="VFU15_16520"
     CDS             1454..3604
                     /locus_tag="VFU15_16520"
                     /inference="COORDINATES: protein motif:HMM:NF026253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HEU4719450.1"
                     /translation="MAPVCKPKVYGLQNGRNEKVLDATYAFLVAQESGTEWIALKDGK
                     DGVINQKGEWIIKPDFETIMQYENGFAVAGKKVKREDAGLVDDYSGYYYGDSAIAFGI
                     IDQSGAWVVEAKYAYLRMGDDGTLLYMDAGHFGYLNADGSTLIRAQYDIAVPMSNGTG
                     IIAEKQKSGYNAGYGSYYNPNLSGNEREGRYYLIDHAGNKLNQEPYEYIRGYKEKRAA
                     FNKGGFWKKKSAYDYNPARLRGGKWGFLDPSGKEIIPAIYDYVYDFSNGKAKVQLGAR
                     TFWIDAEGKECAPPDPGAEDTKISVYCEPGFYGYIDLKGNWVIEPQYLDAGNFSEGLA
                     SAVTLKASDMDCMQKGEGDDDADDSNGDDDQSSFDILRKGITGNGYDVYSKGLTYSVK
                     HRRLYGYIDPQGVMVIPARYERAEEFSCGRAYVCFRNKWGVIDRSGNWIMAPVLDWLS
                     YMDYDYSMPNEGDEGSYSPLGYSAGATENLYRFSENFGVIYKYGKYGFIDTTGNIVVA
                     PVYDKVQPFHNGLAAVRYNGLWGYIDKTGKEVIPRQFISAKSFGAEGLAPAALSPRKA
                     NAVSDDQQAYENDGINYFGYIDTKGNWVIKPQYTYAGVFSDGLADVAVDFGKRGYINA
                     SGNFVITPRFDYTGSFRDGYAVVKMRMFKAVYVDKTGKVSKEYSLEKPPRDKSVPLNA
                     DYNSSGRGGYVNEKGEEVIAHQFASCGKFAKVTR"
BASE COUNT          963 a          873 c         1013 g          907 t
ORIGIN      
        1 tttccctgga gttttttcag ctcatgtttg gatagcagga gcttgcggtc gcgcttcggc
       61 tcgtggttgt aatgcgtgcc tttttcatat tcgctgatct gcacattgcg caggaagagc
      121 tcgccccgct caaaaacgca gaagccgtcc gttatgttcg cttttccctg gcgcagggat
      181 ttgatctcag tccccgtaag cacgagtccc gcgatgaatt tttccaggaa agcatactcg
      241 aaagacgctt tccggttacg gatgtctatt tgatcggatg gcagcaatga aaatcaggat
      301 gaaaaaaaac gacctgcagg gagttccgga gatttgtttt tcagatcgtt tttttggctt
      361 ggtgcccagg gggagactcg aactcccaag ggatattccc aacggcttct gagaccgcaa
      421 cgtttaccaa tttcgccacc tgggcattga aatgatgaat tcaaaaggac aaatgatgaa
      481 ataaaaagga gcagtacttc atcatacaga attcatcact tagcattcaa ttgtgcccag
      541 gactggattc gaaccagcac gccgtgtggc gccaccccct caagatggtg cgtctaccaa
      601 tttcgccacc tgggcaggga taattaataa gctctgaaga acgctctcct gcgcttgttt
      661 cgcaaaagga tgacaaaagt aatacttttc tccaagtgtg aaaatgcaga aatttttccg
      721 gcgccccatt ttgttaaaga atcagtaccc ggtaaataaa acggggaatt cattcgtacc
      781 tttttatgat ttttgcacat taattgatca tttcagcgtt gtaaagacat gagaaagacg
      841 attctggtcg tgttcctgct ggtggcgggt atgaaggtgg acgcggcaaa atatttcatt
      901 ttcccggata cgacgggtac gccatcggtt tgtgacagca ttgtaaccta tgccaccagt
      961 ttcctgggga ctccttacca ttacgggtgc agctccccga aggccggttt cgactgctcc
     1021 gggtttacct ggtacgtttt cggtcattac gggatagata tcccgcggtc cgcgcaggcg
     1081 tatgcgacgg tgggaaagga ggtttcgctc agcgaatgcc gtaaaggcga tatcatcctc
     1141 ttccgcggca cccatcccgg agaaaaaaga gtggggcatg tgggcattat catctccgga
     1201 aaaggcgagc ccgtgaaatt cattcacgcg tcatcttccg ataatcaccg cggtgtcgtt
     1261 atcaccgatt attattccag ccattacccc gcgcgtttca taaaggtgat ccgcattatt
     1321 cccgaagaaa aaaagtaaac gttcaatgaa aaaaaggctt accccaaaag cttttgcttt
     1381 tcccgttctt atcctttccc tggccggatt ctgtttctct ttctacccgg acagcggaaa
     1441 aaatgaaata tggatggctc ctgtatgcaa gccgaaagta tacggcctgc agaacggcag
     1501 gaatgaaaaa gtgcttgatg ccacgtatgc ttttcttgtc gcacaggaat cgggaacgga
     1561 atggattgcg ctgaaagacg gcaaagacgg cgtcatcaac cagaaagggg agtggatcat
     1621 caagcctgat tttgaaacga tcatgcagta tgaaaacggg ttcgccgttg ccgggaaaaa
     1681 agtaaaaaga gaagatgccg gattggttga tgattactcg ggttattact atggcgactc
     1741 ggctattgct ttcggcatta tcgatcagag cggtgcctgg gtggtggagg cgaaatacgc
     1801 ctacctgcgc atgggcgatg acgggacatt gctttacatg gatgcgggcc atttcggtta
     1861 cctgaatgca gacggatcga cgctgatccg cgcccaatac gacattgccg tgcccatgag
     1921 caacggcacg gggatcattg ctgaaaagca gaagagcgga tacaatgcag gatacggaag
     1981 ctattacaac cctaaccttt caggcaatga gcgcgagggc aggtattacc tcatcgatca
     2041 tgccgggaac aagctgaacc aggagcccta cgaatacatc cgcgggtaca aagaaaaaag
     2101 agcggcgttc aacaaaggcg gtttctggaa aaagaaatcg gcgtatgact acaacccggc
     2161 ccggctgcgg ggcggaaagt ggggcttcct cgatccttcc gggaaggaaa tcattcccgc
     2221 catttacgat tacgtctatg atttcagcaa tggaaaagca aaagttcagc tgggcgcgcg
     2281 tacattctgg atcgatgcgg aaggaaagga atgcgccccg cctgatccgg gtgcggagga
     2341 tacaaagatc agcgtttact gtgagcccgg tttttacggc tatatcgatc tgaaaggaaa
     2401 ctgggtcatt gagcctcaat acctagatgc gggcaatttc agcgagggac tggcttccgc
     2461 tgttacgctg aaagcttccg atatggattg catgcagaaa ggagaagggg atgatgatgc
     2521 tgatgattcc aatggagacg atgatcaaag cagcttcgat attttacgga agggaattac
     2581 aggaaatgga tacgatgtgt acagcaaggg acttacttat tctgtaaagc accgcaggct
     2641 gtatggttat atcgatccgc agggcgtaat ggtgattccc gccaggtatg aacgcgcgga
     2701 agaattcagc tgcggaagag cgtatgtctg tttccgcaac aaatggggcg ttatcgaccg
     2761 cagcggaaac tggatcatgg cgccggtgct tgactggctc agctatatgg attatgatta
     2821 ttccatgcct aatgaaggcg atgaaggctc ttattctccg ctgggttatt ctgccggtgc
     2881 caccgaaaat ctctaccggt tcagcgagaa tttcggcgtc atttacaaat acggcaaata
     2941 cgggttcatc gacacaaccg ggaacattgt ggtggcgccg gtttatgata aagtgcagcc
     3001 gttccacaac gggcttgcag ccgtgcggta caacgggctg tggggatata tcgacaaaac
     3061 ggggaaagag gtgatcccgc ggcaattcat ttcagccaaa tcattcggtg cggaagggct
     3121 ggcgcctgct gctctctctc ctagaaaagc gaacgcagtt tccgatgatc agcaggcata
     3181 cgagaatgac ggcatcaatt atttcgggta catcgatacg aaagggaact gggtgatcaa
     3241 accgcaatac acgtatgcgg gtgtgttttc ggacggcctg gccgatgttg cggtcgattt
     3301 cgggaagcgt ggttacatca atgcctcggg aaatttcgtt atcacgccgc ggttcgatta
     3361 taccgggagc ttccgggatg gctatgcggt tgtgaaaatg cgcatgttca aagccgttta
     3421 tgtcgacaag acaggaaaag tgagcaagga atattcgctt gagaaacctc cgcgcgacaa
     3481 atccgttccg ctgaatgccg attacaattc ttccggccgc ggcggttatg tgaatgaaaa
     3541 aggcgaagaa gtgatcgctc accagttcgc ttcctgcgga aagtttgcaa aagtcacacg
     3601 ctgatcaacc ggtaaaaccg gtcggaacaa cgacgttcag cacctgcttt tccaccgtgg
     3661 caatgatctc tgttcctgca ttgtcgggct cgccgtcgta atgcgaaggt cccggtgcgc
     3721 tgcgttcgat ccgtatttct tttccgctgg ctgaaa
//
LOCUS       DASTSF010000584         2726 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1330427,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000584 DASTSF010000000
VERSION     DASTSF010000584.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2726
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1330427"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(91..537)
                     /locus_tag="VFU15_16525"
     CDS             complement(91..537)
                     /locus_tag="VFU15_16525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719451.1"
                     /translation="MKKFILFLPLAFLAFSCKKEVSAAPWPSLNGTWEMKSSEYDVYR
                     DAQLSDHPFLPAAEGQNVYDFHPGNTFTQRSTAFPAVNGRYIFRNDSLLLFSDTSSVA
                     TQSFRLVFSSAQRFSLISAQDSATVMHESFSADTPDSLQLNFERLQ"
     gene            884..1201
                     /locus_tag="VFU15_16530"
     CDS             884..1201
                     /locus_tag="VFU15_16530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719452.1"
                     /translation="MKKIIFSAAGIFAMTAAVVIGSSFTAGKKTNVSGEIEFRITNNT
                     GSEFDYCVNGGHNSIDNGSTKVFDYEPGQEFYYVEDDNCGHLWFKATDDMNGKSYNVT
                     DLK"
     gene            complement(1279..2631)
                     /locus_tag="VFU15_16535"
     CDS             complement(1279..2631)
                     /locus_tag="VFU15_16535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014798866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEU4719453.1"
                     /translation="MAKSATIIGAGLVGSLLSIYLAKRGYKVDVFERRPDMRKNKISA
                     GKSINLALSDRGFRGLDGVGIGDEIRKIAIPMHGRFIHNQDGTTAFQPYGKQGQFINS
                     VSRAGINMKLMDLAESMPGVKFHFSERCTNVDTKTGKSVFANEETHASHDFASDLVFG
                     ADGAFSAARLSAMLGHDRFDYKQFYLEHAYKELHIPAGPNGGFLIEKNALHIWPRKSF
                     MMIALPNIDGTFTCTLFMPFEGENSFAQLKTKEDVKKFFDEVFPDAVPLMPTLVDDYF
                     ANPTSSLITVKCFPWTFDSRLALIGDAAHAIVPFYGQGMNAGFEDCVVLDGLITKHKE
                     NWADIFHEYEQLRKPDGDAVADLAIGNFVEMRDKTADPKFLLQKKIEAWFSEKHPDKW
                     IPQYTMVTYRPDIRYSTAMERGQRNEQIMHEVMAEIPGIEKNWQTEEVEKAILSKLEN
                     "
BASE COUNT          665 a          677 c          731 g          653 t
ORIGIN      
        1 gacaggagaa gcaatccgcg ctcgaactga caggagaagc aaacttgcac ccgaatgtgg
       61 gatgcggaat tgatttttta aactaaaaaa tcactgcagg cgttcgaaat tgagctgcag
      121 gctgtcgggt gtatccgcag agaatgattc gtgcatgacc gtggcgctgt cctgcgcact
      181 gatgagcgaa aaacgctgcg cggaggaaaa cacgaggcgg aacgattgcg tggcgacgga
      241 ggatgtgtcg ctgaaaagca ggaggctgtc gttgcggaaa atataacggc cgttcacggc
      301 gggaaatgca gttgaacgct gcgtgaacgt attgccggga tggaaatcat agacattttg
      361 tccctccgcc gcaggaagga acgggtggtc ggacagctgc gcatcgcggt aaacgtcgta
      421 ttccgagctt ttcatttccc acgtgccgtt cagcgaaggc cagggcgcgg cggaaacttc
      481 ttttttgcag gagaacgcga gaaatgcaag cggcaggaag aggatgaact ttttcatgcc
      541 gcaaaaattc gtttcctgtg ttaaccggga ataagcggca tgtaaactcg gcgggaagaa
      601 attgttgggg aaaggtcaat atttacagat aagagacggg aacccggttt attttcaaat
      661 atgcgtttac agggaagagg tgacagtgtg tcgcccgtcg cccgggataa agcctgcatt
      721 tacgcgacat aacgcctttt atggtgaaag aaaaggtccg ccggttgccg gcacccggaa
      781 tttcacggta aaagttcaaa atgatccgct gtgaaacgat ccatcgcgtt tgtttgcgtt
      841 tataaaatcc tatcttgcat actcaaacca atcccaactc cagatgaaga aaattatctt
      901 ttccgcggcc ggtatcttcg cgatgaccgc tgcggttgtc atcggaagtt cgttcacagc
      961 cggcaaaaaa accaacgttt ccggcgagat cgaattccgc atcacgaaca acaccggaag
     1021 cgagttcgac tactgcgtga acggcggcca taacagcatt gacaacgggt cgacgaaagt
     1081 gttcgattac gagcccggcc aggaatttta ttacgtggaa gacgacaact gcggccacct
     1141 ctggttcaag gccaccgacg atatgaacgg aaaatcgtac aacgtcaccg acctgaaata
     1201 atccgggatc ttctcaatga aaaaatgaaa aagggttgca gaaatgcagc ccttcttttt
     1261 tgaaaaaatt tccggcgcct aattttccag cttgcttaaa atcgcctttt ccacttcctc
     1321 cgtctgccag ttcttttcaa tgccgggaat ttccgccatc acttcgtgca tgatctgctc
     1381 gttgcgctgc ccgcgttcca tggccgtcga ataacggatg tcgggacgat acgtgaccat
     1441 cgtgtattgc gggatccact tgtcgggatg cttttcgctg aaccatgctt cgatcttttt
     1501 ctgcagcagg aatttcgggt cggcggtttt gtcgcgcatc tccacgaaat tcccgatggc
     1561 gagatcggcc acggcgtcac catcgggctt gcgcagctgt tcgtattcgt gaaaaatatc
     1621 cgcccagttt tccttgtgct tcgtgatcag cccgtcgagc acgacacaat cttcaaagcc
     1681 tgcgttcatg ccctgcccgt aaaacgggac tatggcgtgc gcggcatcgc cgatgagcgc
     1741 aagacggctg tcgaacgtcc acggaaaaca tttcaccgtg atgagcgacg atgtcgggtt
     1801 cgcgaagtaa tcgtcgacca gcgtaggcat gagcggaacg gcgtcgggaa aaacttcatc
     1861 aaaaaatttc ttcacgtctt ccttcgtctt caactgcgcg aatgagtttt cgccttcgaa
     1921 cggcatgaac agcgtacacg tgaacgtccc gtcgatattc ggcagcgcga tcatcatgaa
     1981 acttttgcgc ggccagatgt gcagcgcatt tttttcgatg aggaacccgc cgttgggtcc
     2041 ggccgggatg tgcaattcct tgtacgcatg ctcgaggtaa aactgcttgt aatcgaaacg
     2101 gtcgtggccc agcatggcgc ttaagcgtgc cgcggaaaat gcgccgtcgg cgccgaatac
     2161 aagatcagaa gcgaaatcat gcgaagcatg cgtttcttca ttggcaaaaa cagattttcc
     2221 tgttttcgta tcaacgttcg tgcatcgctc gctgaaatgg aatttcacgc ccggcatcga
     2281 ttcggcgaga tccatcagct tcatgtttat tcctgcacgc gaaacggaat tgatgaactg
     2341 tccctgtttt ccgtacggct ggaaagcggt cgttccgtcc tggttgtgaa tgaaccgtcc
     2401 gtgcatcggg atagcgatct tccgaatctc gtcgccgatg cctacgccgt cgagtccgcg
     2461 gaagccgcgg tcgctcagcg cgaggttaat ggattttccc gcgcttattt tattcttccg
     2521 catatcggga cgccgttcga acacatcgac tttgtaaccg cgtttggcga gatagatgga
     2581 aagcagggag cctacaagtc ctgcaccgat gatcgtagct gattttgcca tggaaattgt
     2641 attgttacgc aaagaaaatc atttcggcgg atcaggaaca tgacaattat cggttgtcct
     2701 gccggggaag aatcgcttat ttttga
//
LOCUS       DASTSF010000585         3084 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_788135,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000585 DASTSF010000000
VERSION     DASTSF010000585.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3084
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_788135"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1180)
                     /locus_tag="VFU15_16540"
     CDS             complement(<1..1180)
                     /locus_tag="VFU15_16540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719454.1"
                     /translation="MGRQYSYERIDRRMEDMDDWYLDEKRKMAGGKKTVQAKLEISQP
                     GDKHEQEADAVARNVVNGGKTNMPSITPVSDSISTARKDKPEYTPFEVQFTAYSIVNG
                     DPDVKKVILAIQKIDEDTLWRFMKPDSEFLPQLIFSDKISGGGSTPEKFGKRSHKKIY
                     IASSLPFPVAVARYLHELNHYIDIWLAGKSAQIKGRNLAQARDERGEGIASPDLVSTD
                     EQKNAVGELVTSVNKDLIGEIENKPKPEDTYDPEDSGREKTLKKNYRRWAKKHKNETW
                     DEFLEQQKKWLDQEAISSYGRWGAKADPLTHVVTSSKVELGYVFEKSLYYKKNPDAAP
                     GEWMDKFDGWFEGAYASQLSIPSLTKASNVLQKKESNGTAGGGETASPQFTSDLESTK
                     G"
     gene            complement(1199..2647)
                     /locus_tag="VFU15_16545"
     CDS             complement(1199..2647)
                     /locus_tag="VFU15_16545"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU4719455.1"
                     /translation="MTLSTDPLNQLLKSNEALNDSGTILNGEALAENDLALEKEFRFL
                     NDFFAARIKFAEDSTQLPSVMERIRVINHAPSKNGVLNGLAEMLDLSVIERILLLLVL
                     EPHYNPVGVSKRLSSLAESNPDLMDFFSYYVDPYSKNRYPTLQSALFLLAGDNPSEWR
                     ECEQEILFRGKLLREQIIVLRDPDHRERVGNRLNQLIDLAPEYVDYLLHGQRPRPDFG
                     RAFPASWVYTNLSWDQLVLNPLTLAEIGDVMDWVSHGRETLSISGGKINKSFPVLFYG
                     PPGTGKSFTAKLIGKEYGKDVFRIDLSMIVSKYIGETEKNLAHLFDRAEGKDWILFFD
                     EADALFGKRTGISDSKDKWANLEVSYLLQRMEEYQGLCILATNLKHNLDAALTRRFQA
                     IIHFPFPKPAERALIWEKSLPPGFRYPENISFEKLGAYDLSGGAITNVIKASCVKAVK
                     RGDRVLSTKDLSRFIKLEYSKDTRTPVRSNNN"
     gene            complement(2699..>3084)
                     /locus_tag="VFU15_16550"
     CDS             complement(2699..>3084)
                     /locus_tag="VFU15_16550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719456.1"
                     /translation="TAKPSYAFDIHAAGSGKYSATPRLEIGWSADLNALFSSATEPNF
                     EASGSVTVMRKVNVFKKSGGVYITGLGKYSSTEPDVIGGKAGIKIPIGDQFILGGEYY
                     LNYDNEHPLNLWQPSQGLRIYLLHR"
BASE COUNT          648 a          772 c          836 g          828 t
ORIGIN      
        1 cgcctttcgt gctttccagg tcggaggtaa attgtggtga tgccgtttcg cctccgccag
       61 ccgtaccgtt gctttctttt ttctgcaaaa cattactcgc ttttgtaagc gagggaatgg
      121 aaagctggct ggcataggcg ccttcaaacc aaccgtcgaa tttgtccatc cactctccgg
      181 gcgcggcatc ggggtttttc ttgtaataaa gtgatttttc gaagacatag ccgagctcca
      241 ctttcgaaga ggtgactaca tgtgtcagcg gatcggcctt ggcgccccat cgaccgtaag
      301 agctgatggc ctcctggtca agccattttt tctgctgttc aaggaattcg tcccatgttt
      361 cgttcttatg cttctttgcc catctccggt agtttttctt gagtgttttc tcccggcctg
      421 aatcttcagg atcatacgtg tcttccggtt tcggcttgtt ttcgatttca ccgatgaggt
      481 ccttgttcac cgaagtaacc agttcaccaa cggcgttttt ctgctcatct gttgaaacaa
      541 gatcggggct tgctattcct tcgccgcgct catcacgcgc ctgcgccagg ttgcggcctt
      601 ttatctgcgc actttttccc gcaagccaga tgtcgatata atgattgagc tcgtgcaggt
      661 aacgtgcaac ggcaaccgga aagggcaggg aggaggcgat gtatattttt ttgtgcgaac
      721 gctttccgaa tttttccggt gttgaaccac cgccgctgat cttatcgctg aaaataagct
      781 ggggcaggaa ctcgctgtcc ggtttcatga atcgccacag cgtgtcttca tcgattttct
      841 ggatggcgag aatgaccttc ttcacgtcgg gatcaccgtt cacaatggag taggctgtga
      901 actggacttc gaaaggggtg tattccggct tgtctttccg cgctgtcgaa atggaatcgg
      961 aaaccggcgt aatcgaaggc atattggttt tgcccccgtt caccacattc cttgccactg
     1021 cgtccgcttc ctgctcgtgc ttatccccgg gctgtgagat ttccagcttc gcctgcacag
     1081 ttttttttcc gccggccatt ttcctctttt catcgaggta ccagtcgtcc atgtcttcca
     1141 tgcgacgatc gattctctcg tatgaatact ggcggcccat aagcggatct gagtgatctt
     1201 aattattgtt gctgcgcacc ggagtgcgcg tgtccttgga atattcaagc ttgatgaagc
     1261 ggctgaggtc ttttgtgctg agcacacgat cgccgcgctt cacggctttc acgcaggatg
     1321 ctttgatcac gttcgtgatg gcgcctccgc tgagatcata cgcgcccaat ttttcaaagc
     1381 tgatgttttc cgggtagcgg aagccgggcg gaagactttt ctcccagatc agcgcccgtt
     1441 cagcgggttt cgggaacggg aagtggatga tggcctggaa acggcgtgta agcgccgcgt
     1501 cgagattgtg cttgaggttc gtggcgagaa tacagagtcc ctggtattct tccatgcgct
     1561 ggagcaggta agacacttcg aggttcgccc acttgtcttt cgaatcgctg atgccggtgc
     1621 gtttcccgaa caatgcatcc gcttcatcga agaacaggat ccagtctttc ccttcggcgc
     1681 ggtcgaaaag atgcgcaagg tttttttccg tttcaccgat atattttgaa acgatcatgg
     1741 aaagatcgat gcggaaaaca tctttgccgt attctttccc gataagcttc gcggtgaagg
     1801 actttcccgt tccgggaggt ccgtaaaaca ggacaggaaa acttttattg atctttcccc
     1861 cggagatgct cagcgtttcg cgtccatgac tgacccagtc catgacatca ccgatctcgg
     1921 caagcgtgag cgggttcagc acgagctggt cccatgacag atttgtgtag acccacgatg
     1981 cggggaaggc gcgaccgaaa tcaggccgcg gccgctggcc gtggaggaga taatcgacgt
     2041 attcaggtgc gagatcaata agctggttga gacggttgcc cacacgctca cggtgatcgg
     2101 gatcgcgcag cacgatgatc tgctccctga gaagtttgcc acggaacaga atttcctgtt
     2161 cgcattcgcg ccattccgaa ggattatcac cggcaagaag aaacagtgcc gactgaagtg
     2221 tgggatagcg gttcttcgaa tacggatcga cgtagtagct gaaaaaatcc atcaggtcgg
     2281 gattgctttc tgcaagcgag gacagtcttt tgcttacacc gacgggattg taatgcggtt
     2341 cgaggacgag gagaagaagg atgcgttcga tcacactgag atcgagcatt tccgccaggc
     2401 cgttcaggac gccgttcttt gaaggagcat ggttgatgac gcggatccgc tccataacgg
     2461 aaggcaattg tgtgctgtct tccgcaaact tgatccgggc cgcgaaaaaa tcgttgagaa
     2521 accggaattc cttttccagc gcgaggtcgt tttcggcaag agcttcaccg ttcaggatag
     2581 taccggagtc gttgagtgct tcattacttt taagaagctg gttgagagga tcggtggaaa
     2641 gtgtcataaa aacgggtgtt tgattcgtga ataaacgaaa gccggtttgt tataaagatc
     2701 atctgtgcag caggtaaatg cgtagtccct ggctcggctg ccacaggttg agaggatgtt
     2761 cgttgtcata gttaagatag tattcaccgc ccaggataaa ctgatcgccg atggggatct
     2821 tgatcccggc ttttccgcct atcacatccg gttccgtgct gctgtatttg ccaaggccgg
     2881 taatgtaaac tcccccggat ttcttgaaca catttacttt tcgcataacg gttacggatc
     2941 ctgaagcttc gaagttcggc tctgtggcag aagaaaacag cgcgttcagg tcggccgacc
     3001 agccgatctc cagcctgggg gtggcggagt atttaccact gccggccgca tgtatgtcaa
     3061 acgcataaga aggtttggca gtgg
//
LOCUS       DASTSF010000586         5744 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1300283,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000586 DASTSF010000000
VERSION     DASTSF010000586.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5744
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1300283"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1987
                     /locus_tag="VFU15_16555"
     CDS             <1..1987
                     /locus_tag="VFU15_16555"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEU4719457.1"
                     /translation="YVNNTLILSAWPFRSTTATSVDHVYMHSLNAASVDYDDIIIGAS
                     PVTVTTSQTNITCYAGSNGSASVTAGGGNGVYSYSWSSGDVTATASGLSAGTYTCTVT
                     DGLGCFTTASVSITEPAMISGTTNVTDITCNGNGDGAIDLTPGGGTPGYTFQWSNGAT
                     TEDVSGLQQGSYNVYITDMNGCLDSLTGILVQEPAVLSANATSTGITCPGGTDGSGTV
                     APSGGTGAYTYLWTPGNATTAGITNLTAGSYTCVVTDANGCSASASINLTAPPAFVTS
                     IIASDVLCNGGSTGTADISVSGGTPGYSYSWSNGDMTSTASGLSAGSYTCTVTDNLGC
                     VTTRSVTITEPSPLSAVVMDTNATCSNSCNGETNIVPSGGVAPYSFSWSPGGQTTQSL
                     QNLCPGTYTCNWMDANGCAMTSVATISAPPAIVLGAVTTNPTSCVVNNGSINLTASGG
                     TPSYSYAWSNSVTTEDINGLSAGNYSVVVTDASGCSDSIAMTLSPPPSPTVTMSVSQD
                     TICANDASLALTGATPAGGTWSGPGVTGSTFNPGSAGNGLAFIMYTYTDPVSGCAGTS
                     SDSIFVSACTGVQENTAAGNLQVYPNPNNGSFTIEYGDFRNVTALVLNNSLGEAVGEW
                     KMNSAKLAIDMQSLPAGIYFLQMTTPEGIVTTRIAKN"
     gene            2120..3196
                     /locus_tag="VFU15_16560"
     CDS             2120..3196
                     /locus_tag="VFU15_16560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719458.1"
                     /translation="MKTIFLFFLVFPLALPAQLSKIKPGMKLDDARKQFPGMKPDYTG
                     MTSWVYKKDTIGMIAGKATYKVMRDTVRSYNFLGDEVPGPCPGNAGADTADYSSFMQN
                     VRRQAAFYTKIFGKPVLSMDGPGITSSELNYDVVFDFRWKFNGDELTMQVTRPKERTK
                     PQVNGAADVHGCAQCCNYTLEIDVKGNGTGMKNPFCLGMTKNEFKKAHPEMSLQVNDY
                     PDKWIMPDTLLTKDGLWRMDFEDGKLVAFNLSIYDGKIYGTDADAAYARLLGRAKELT
                     KEAKKKYGKADSLMTDVPDKRPPVVTDSHGLMDRTDYKASWNVDGKVLVICINERAGG
                     MGGGREPVFNLLVTYPFKEDHAGR"
     gene            3221..4273
                     /locus_tag="VFU15_16565"
     CDS             3221..4273
                     /locus_tag="VFU15_16565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719459.1"
                     /translation="MKKCAVLLSVFVPVIVMAQLDRIRPGMPEADFQKQFPAAQYVPM
                     KSWINSPHTEDGIDGNVSYYAESDTVRTYQFTSNAVAGPCAEYPGADSAAVEKLLEVA
                     RTLSGHYSDIFGTPSAHSSAGLTIPPPQLEEVNVFYSKWKTPLYEITVSVHRWQKLKD
                     DPRMEINGPHTPVKASCDYYFEVSAMGKGLQIKGEFGIGITTDEFLAQHPDYAGKISA
                     EAAYWQLDDTLTSNYGSWRFTFVNSALAAFSLDIYDGPHYGNGMDSGYAAVRERALAL
                     VADAEKQFGKADSASKKFPEKLHSRKPSNNYYRDVYYEAKWKKGKDGMEIVADEFGGG
                     KTGAPVIHLHLYYGPE"
     gene            complement(4276..>5744)
                     /locus_tag="VFU15_16570"
     CDS             complement(4276..>5744)
                     /locus_tag="VFU15_16570"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HEU4719460.1"
                     /translation="NEVRLSRLPDALTESAKKHGGRIALRETGGASVSYTEFLRRAES
                     LADFLSRHGIGPDKRVAILFPKSIDAVIAIAATVLSGATYVPIDPELPAERVKLLLGD
                     SDPHALLAHADHFPSEIGSGELLPGPVYGRAAFFPTESVDTFPDLISILYTSGSTGAP
                     KGVCISHGNMHAFVSWALETFPLSENDHLSSLAPLHFDLSVFDIFCGLISGCCITLFD
                     HKTVKNARMLAKIISEENISVVYATPSLLNMILNFGKPEKFSFEKLRLVLFAGEIFPV
                     AQLHRLIGLWKHARFFNLYGPTETNVCSYFEIPLPPDAERTDPYPIGKMCAHLEGNIS
                     ATGELLVAGANVTPGYRNRDELNANKFTEIGGQRFYHTGDRVEKCEDGNLIYAGRIDR
                     MIKKRGYRIEPGEVESIISGFPGVKDVAVTGAKDEEGFSLLVAFIVTANPDVQAIHAR
                     FMEYGPAYMIPDKVIFPDSIPKTSSGKTDYQKLEEMIR"
BASE COUNT         1378 a         1489 c         1603 g         1274 t
ORIGIN      
        1 atatgtgaac aacacgctga tcctttcggc gtggcctttc cgttctacca ccgccacttc
       61 ggttgatcac gtttacatgc acagccttaa tgcagcttcg gtcgattatg acgacatcat
      121 catcggcgct tcgccggtga cggtaaccac gtcgcaaacg aatattacct gttatgcagg
      181 cagcaatggt tctgcttccg tgaccgcggg cggaggcaac ggggtttatt cctacagctg
      241 gtccagcggc gatgtgacgg ctacggcttc cgggctttct gccggaacgt acacctgtac
      301 agtcaccgac ggactcggtt gcttcactac ggcctcggtc agcatcacgg aaccggcaat
      361 gatctccgga acgacgaacg tgaccgatat tacctgcaac gggaacggcg acggcgcgat
      421 cgatcttacg ccaggtggcg gaacaccggg atatacgttc cagtggagca atggcgccac
      481 aacggaagat gtttccggtc tgcagcaggg cagctacaat gtgtacatca ccgacatgaa
      541 cggctgtctc gattcgctga ccgggatact ggtgcaggaa cctgccgtgc tgagcgcaaa
      601 cgcgacatcg acgggcatca cctgcccggg aggtacggac ggatcaggca ctgttgcacc
      661 cagcggcgga accggcgctt atacttactt atggacgccg ggtaacgcaa cgactgccgg
      721 cattacaaat ctcacagccg gttcatacac atgcgtcgtc actgatgcaa acggctgttc
      781 tgccagcgcc agcataaacc ttaccgctcc accggctttt gttacatcca ttatcgcgtc
      841 ggatgttttg tgtaacggag gttcaaccgg gacagccgac ataagcgtgt caggcggaac
      901 gccgggctat tcttacagct ggtcgaacgg agatatgaca tcaacagctt cgggactttc
      961 cgcaggatca tacacctgca cggttacgga caacctcggt tgcgtgacaa caagatcagt
     1021 cacgatcaca gagccgtcac cgctttcggc tgtggtgatg gatacgaatg caacgtgcag
     1081 caacagctgc aacggggaaa cgaacattgt tccttccggt ggagttgcgc cgtattcgtt
     1141 ttcctggtcg cccggaggac aaacaaccca gtcgctccag aacctttgcc cgggaacata
     1201 cacctgcaac tggatggatg caaacggttg tgccatgaca agtgttgcaa cgatcagcgc
     1261 tcctcccgcg attgtccttg gcgcggtcac gaccaatccc acgagctgcg ttgttaacaa
     1321 cggttcgatc aaccttacgg cttccggcgg aacgccttcg tattcgtacg cctggtcgaa
     1381 cagcgtcacg acggaagata tcaacggcct cagcgccgga aattattccg ttgttgtcac
     1441 cgatgcttcg ggatgctcgg attcaattgc catgacactt tcaccaccgc catcgccgac
     1501 agtgacgatg tctgtttcgc aggacacgat ctgcgcgaac gacgcttcgc tggctttgac
     1561 aggagcgacg cctgcaggcg gaacatggag cggtccgggc gtgaccggtt ctacgttcaa
     1621 tccaggttct gccgggaacg ggctggcctt cattatgtat acttataccg atccggtgag
     1681 cggttgtgcc ggaacgtcaa gtgacagcat tttcgtgtcc gcgtgcacag gagttcagga
     1741 aaatacggcc gcaggaaatc tccaggttta cccgaacccg aacaacggtt catttaccat
     1801 cgagtatggc gatttcagga atgtgacggc cctcgtgctg aataattcgc tcggtgaagc
     1861 ggtgggcgaa tggaaaatga acagcgcaaa gctggcgatc gacatgcagt ctttgcctgc
     1921 cggaatttat ttcctgcaga tgacgacacc ggaaggtatc gttacgacgc gcatcgcaaa
     1981 aaattagaag aagtaacttc atctcacgat gccggttgga aacgaccggc attttttttg
     2041 actcattccg gacgcttttg cccgttcccc ggaattgcca taaattgaga agaaaataaa
     2101 ccggctcttc attcacccca tgaaaaccat tttcctcttt ttcctggttt ttccgcttgc
     2161 acttcccgca cagctttcga aaataaaacc gggcatgaag ctcgatgacg cgcggaaaca
     2221 gtttcccggc atgaagcccg attatacggg aatgacttcc tgggtgtata aaaaagacac
     2281 gatcggaatg atcgcgggaa aagcgacgta caaagtgatg cgcgataccg tccgctcgta
     2341 caattttctt ggcgacgaag tgccgggccc ttgtcccggg aacgcgggcg cagatacagc
     2401 ggactattcc tcctttatgc aaaatgtgcg caggcaggcg gcgttctaca cgaaaatttt
     2461 cgggaaacct gtgctcagca tggatggtcc cggaattacg tcttcggaac tgaattatga
     2521 tgttgtgttt gattttcgct ggaaattcaa cggcgatgag ttgacgatgc aggtgacgcg
     2581 gccgaaagaa cgcacaaaac cgcaggtcaa cggtgccgcg gacgtgcacg gatgtgcaca
     2641 gtgctgcaac tatacgctgg aaatcgacgt aaagggaaac ggaacgggga tgaagaaccc
     2701 gttctgtctc ggcatgacga agaacgaatt caaaaaagcg cacccggaaa tgtcgctcca
     2761 ggtgaatgat tatcccgaca aatggatcat gccggacacg ctgcttacga aggacgggtt
     2821 atggcgtatg gattttgagg acgggaagct cgttgcattc aatctcagca tttacgacgg
     2881 aaaaatttac ggaaccgatg ccgatgccgc ttatgcaagg ctgctcggtc gcgcaaagga
     2941 gctgacgaag gaagcgaaga aaaaatacgg gaaggccgac agcctgatga cagatgtgcc
     3001 ggataaacgc ccgcccgtcg tgaccgattc gcacggtctc atggacagga cggattataa
     3061 agcatcctgg aacgtcgatg gcaaagttct tgtgatctgc atcaatgaac gggcaggagg
     3121 gatgggcggc ggacgggaac ctgtttttaa tctgctggta acctatcctt tcaaagaaga
     3181 tcatgcggga aggtgaaaga attttctata cttgcgctgt atgaaaaaat gtgctgtttt
     3241 gctgagtgtt tttgtgccgg tgatcgtaat ggcgcagctg gatcgtattc gtcccgggat
     3301 gccggaagct gattttcaga agcagtttcc cgccgcgcag tacgttccca tgaagagctg
     3361 gatcaattcg ccccatacag aagacggtat tgacggcaac gtaagctact acgcggagag
     3421 tgataccgtg agaacatacc agttcacttc caacgcggtg gcaggtccgt gcgctgaata
     3481 tccgggcgcc gacagcgcgg cagtggagaa attgctggag gtggcgcgta cgctcagcgg
     3541 tcattacagc gatattttcg gaacaccgtc cgcgcattca tcggccgggc tcaccattcc
     3601 gccgccacag ctcgaagaag tgaacgtatt ttattcgaag tggaagacgc ccctttacga
     3661 gatcacggtt tccgtgcatc gctggcaaaa gctgaaggac gatccgcgca tggagatcaa
     3721 cggaccgcat acgcccgtta aagcttcgtg cgattattat ttcgaagttt ccgccatggg
     3781 aaaagggctg cagatcaaag gcgagttcgg gatcggtatc acgacggatg aatttctcgc
     3841 gcagcacccc gactatgccg gaaaaatttc tgccgaggcc gcttactggc aattggatga
     3901 tacgctgacc agcaattacg gttcatggag attcacgttc gtgaacagcg cgctggctgc
     3961 tttttcgctc gacatttacg acggacctca ttacggcaac gggatggatt ccggctatgc
     4021 tgctgtaagg gagcgggcat tagcgctggt tgcggatgct gaaaagcaat tcgggaaggc
     4081 ggacagcgct tcgaaaaaat ttcccgaaaa acttcattcg cgcaaacctt cgaacaatta
     4141 ttaccgtgat gtttattacg aggcgaaatg gaaaaaagga aaagacggga tggaaattgt
     4201 tgcggatgag tttggcggag gaaaaacggg agctcccgtg atccacctgc acctgtatta
     4261 cggtccggaa taaattcagc ggatcatttc ttccagcttc tggtaatctg tttttccgct
     4321 gcttgttttg ggaatgctgt ccgggaagat cactttgtcg gggatcatgt acgcgggacc
     4381 gtattccatg aaacgggcat gaatggcctg cacatccggg ttggccgtga cgatgaaagc
     4441 aaccagcagc gaaaagcctt cttcatcttt cgcgccggtt acagccacat cttttacgcc
     4501 gggaaaaccg gaaatgatcg attccacctc tcccggttct atgcgatagc cgcgcttctt
     4561 gatcatgcgg tcgatgcggc ccgcgtaaat aagatttcca tcctcgcatt tttcaacgcg
     4621 atcgcccgtg tgataaaaac gctgcccgcc gatctccgtg aatttatttg cgttaagctc
     4681 atcgcggttc cggtacccgg gtgtgacgtt cgcgccggca acgagtagct cgcccgtcgc
     4741 cgagatattt ccttcgaggt gcgcgcacat ttttcctatc gggtaaggat cggtccgttc
     4801 cgcatcaggc ggaagcggga tctcgaagta cgagcagacg tttgtttccg tcggcccgta
     4861 caggttgaag aaacgtgcgt gcttccacaa accgatcagc cggtgcagct gcgcgacggg
     4921 aaaaatttcc cctgcgaaaa gcacaagccg caatttttca aatgaaaatt tttccggctt
     4981 gccgaagttc aggatcatgt tcagcaggct tggtgtcgcg tatacgacgg aaatgttttc
     5041 ctcgctgatg atcttcgcca gcatccgcgc attcttcacc gttttatgat cgaagagcgt
     5101 gatgcagcac ccggaaatga gcccgcagaa aatgtcgaac accgaaagat cgaaatgcag
     5161 cggggccagc gaagaaagat gatcgttttc tgaaagcggg aatgtttcca gcgcccagct
     5221 gacgaatgca tgcatgttcc cgtgtgaaat gcacacgcct ttcggcgcgc cggtcgatcc
     5281 ggatgtatag agaatactga tgagatcagg aaaagtatcg acgctttctg ttgggaaaaa
     5341 agccgcgcgg ccataaacgg gaccgggaag cagttcgccg gagccgatct ctgacgggaa
     5401 atgatccgca tgtgccagca gcgcatgtgg atccgaatcg ccgagaagaa gttttacgcg
     5461 ttcggccggc agctccggat cgatgggtac gtaggtggcg ccggacaaaa ccgtggccgc
     5521 gatcgcgata actgcatcga tgcttttggg aaataaaatg gcgacgcgct tgtcggggcc
     5581 gatgccatgg cgggaaagga aatccgcgag tgattcggcc cggcgaagga attccgtata
     5641 ggaaacagaa gcgccgcccg tttcgcgcaa tgcgatccgc ccgccgtgtt ttttcgcgga
     5701 ctccgtaagt gcatccggaa ggcgtgacag cctcacttca tttt
//
LOCUS       DASTSF010000587         4216 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1461593,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000587 DASTSF010000000
VERSION     DASTSF010000587.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4216
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1461593"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1112
                     /locus_tag="VFU15_16575"
     CDS             <1..1112
                     /locus_tag="VFU15_16575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016197519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEU4719461.1"
                     /translation="KAEGSHITDTEGKKYIDLISGISVSNVGHCHPKVVAAIKAQVDR
                     YMHVMVYGEYIQSPQVKLAEKIASLLPPTLNCSYFTNSGAEAIEGAMKLAKRITGRAE
                     IISFENAYHGSTQGALSIMGSEFFKTSFRPLLPGTKRLRFNHAEDLSQITEETACVFA
                     EPVQGEAGAIPAQEGFLEKLRKRCDETGALLVFDEIQTGFGRTGKLFGYMHSGVIPDV
                     IVFAKGMGGGLPIGAFVSSRENLSAFTENPVLGHITTFGGNPVCCAAALATVNAIVEE
                     NLVAGVAEREAVISAILGKSGEIHLRGKGLLYAAVLANAEKVQRVIQHCLQHGVITDW
                     FLFCDNALRIAPPLNIPLNDLNYSCEVIRDGVNRF"
     gene            1160..1819
                     /locus_tag="VFU15_16580"
     CDS             1160..1819
                     /locus_tag="VFU15_16580"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008241656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase family protein"
                     /protein_id="HEU4719462.1"
                     /translation="MKIHREGTFTITVTIIFILAVNTGMFLIFPSFGVWNAIALVLTL
                     ALLYIILQFFRNPPRVTPSNPEHIIAPADGKVVVMEEVEEPEYFQGKRLQLSIFMSPF
                     NVHVNRYPIGGTVSYVKYHKGLYLVAWHPKSSTDNERSTVVVKDEKGREVLFRQIAGA
                     LAKRIVCYPKAGDAAVQGNEMGFIKFGSRVDVFLPTGTKVNVKLNETVRGGETVIASF
                     S"
     gene            1928..2203
                     /locus_tag="VFU15_16585"
     CDS             1928..2203
                     /locus_tag="VFU15_16585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719463.1"
                     /translation="MKKLFVILALAGMTVGASASAINVMTGSHIVVVKGDDDKKKNKK
                     KKKDCCANNQNGATCTHDGKGNCCKDKDKCQDKDMNKQQDDSKKDPK"
     gene            complement(2229..2948)
                     /locus_tag="VFU15_16590"
     CDS             complement(2229..2948)
                     /locus_tag="VFU15_16590"
                     /inference="COORDINATES: protein motif:HMM:TIGR02254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HEU4719464.1"
                     /translation="MAVYKHLFFDLDRTLWDMERNARETLVELYHKYDLASRGIDSAE
                     DFVDHYNHYNHLLWDRYQKRIIDKATLRALRFKQSLAHFGVHDEELAANFDRDYISEA
                     PNKKNLIPGTIELLESIKEDYRLHIITNGFPEVQHHKLSRSGLESYFETIITSEGCGY
                     AKPDARIFSYALKKAKAVAEESLMIGDDLNIDIVGAREAGWHQVFFNPSGGQHKEKVT
                     YEIRRLKELQDMLQKKSPSQG"
     gene            complement(2950..3468)
                     /locus_tag="VFU15_16595"
     CDS             complement(2950..3468)
                     /locus_tag="VFU15_16595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719465.1"
                     /translation="MDANQVKHWLEVLEVALIASVKFLFAPFEAERYHMGFGEAFGVT
                     TFGGLVGILAFYYAGSNITTWWRHVVSIIKSVFLRRPASVIERKPPRSFTRTRRFIIG
                     VKMKFGLTGIAVITPAIISIPIGTIVAAHFFRKRKRVLLYLIASLLFWSLVLNALAQI
                     LQLSQYIPYQAN"
     gene            complement(3475..4122)
                     /gene="upp"
                     /locus_tag="VFU15_16600"
     CDS             complement(3475..4122)
                     /gene="upp"
                     /locus_tag="VFU15_16600"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEU4719466.1"
                     /translation="MINDLSKQSSVLNNYMAELRDVSVQKDSMRFRLNIERIGKIIGY
                     EISKKLEYVTTEVTTPLGIAQVPVLKEQPVVATILRAGIPLHYGILSILDKAENAFIS
                     AYRRHHKDGSFDVHVEYLASPSVEGKELILCDPMLATGTSMVLAYQAILRHGKPRHIH
                     AVSVISSLFGIDYLRKNLPENVTIWTCAVDDELTAQSYIVPGLGDAGDLAFGEKL"
BASE COUNT         1032 a         1110 c         1120 g          954 t
ORIGIN      
        1 ggaaagcgga aggaagccac atcaccgata ccgaaggaaa aaaatacatc gaccttattt
       61 cggggatctc cgtgagcaac gtcggtcact gtcacccgaa ggtggtggcg gcgataaaag
      121 cgcaggtgga ccgttacatg cacgtgatgg tttacgggga atacatacag tcgccgcagg
      181 tgaagctcgc tgaaaagatc gcgtcattgc tgccgcccac gctgaactgt tcctatttca
      241 cgaattcggg cgcggaagcg atcgaaggag cgatgaagct cgcgaaacgt attacaggaa
      301 gagcggagat catctcgttc gaaaatgcgt atcacggcag cacgcagggc gcgctcagca
      361 tcatgggcag cgaatttttc aagacctcct tccgcccgct gcttccgggg acaaagcgcc
      421 tgcgcttcaa tcacgcagaa gacctttcgc agatcacgga agaaaccgca tgcgtgttcg
      481 cagaacccgt gcagggcgag gccggcgcaa ttcccgcaca ggaaggattc ctggaaaaac
      541 tccgcaaacg ctgcgacgaa accggcgcgc tgctcgtgtt cgatgaaata caaacgggat
      601 tcgggcggac cgggaaatta ttcggctaca tgcattccgg cgtcatcccg gatgtcatcg
      661 tgttcgccaa agggatgggc ggcggacttc ccatcggcgc attcgtttct tcgcgtgaaa
      721 acctttccgc ctttacggaa aatcccgtgc ttggccacat caccacgttc ggcggaaatc
      781 ccgtctgctg cgctgctgcg ctggctacgg taaacgcgat cgttgaagaa aatcttgtcg
      841 ccggcgtggc tgaacgggaa gcggtcatca gcgctattct cggtaaaagc ggggagatcc
      901 acctgcgcgg aaaaggattg ctttacgcgg ccgttctcgc gaacgcagaa aaagtccagc
      961 gcgtcattca gcactgcctg cagcacggcg ttatcaccga ctggttcctc ttttgcgata
     1021 acgccctgcg cattgcgccg ccgctgaata ttccgctcaa cgacctgaac tattcctgcg
     1081 aagtgatccg cgatggcgtt aatagatttt aaccgttcct tttcttcgca tccgctatat
     1141 ttgccgaaac atttttccaa tgaaaattca ccgcgaaggc actttcacca tcaccgtcac
     1201 cattattttt attcttgcgg tcaatacggg gatgttcctc attttccctt ctttcggcgt
     1261 gtggaacgcg atcgcgctgg tgctgacgct tgcattgctg tacatcattt tacaattctt
     1321 ccgcaatccc ccgcgtgtga cgccgtcgaa cccggagcac atcatcgctc ccgccgacgg
     1381 aaaagtggta gtgatggaag aagtggaaga acccgaatat tttcagggta aacgcctgca
     1441 gctttccatt ttcatgtcgc cgttcaatgt gcatgtcaac cgctatccca tcggcggaac
     1501 ggtgagctat gtaaaatatc acaaaggcct ttacctcgtc gcgtggcacc cgaaatcttc
     1561 gacggacaat gaacgttcca ccgtcgtcgt caaagatgaa aaaggacgcg aggtgctttt
     1621 ccggcagatc gcaggcgcgc tggccaaacg gatcgtctgc tacccgaaag cgggcgatgc
     1681 ggccgtgcag ggaaatgaaa tgggcttcat caaattcggt tcccgcgtgg acgttttcct
     1741 tcctaccgga acgaaggtaa atgtgaagct gaacgaaacg gtgcgcggcg gagaaacagt
     1801 gatcgcttcc ttttcctgag caggaaaaag ctgttaaaat ctggtaaagt aaccgcgatt
     1861 acgttcatca gtctctttcg cccttatctt tacgctattg aatcaaaaaa ctcaaaatag
     1921 acttccgatg aaaaaactgt ttgtgatcct cgcgcttgcc ggcatgacgg ttggcgcttc
     1981 cgcttcggcc atcaatgtga tgacgggaag ccacatcgtc gtcgtaaaag gcgatgacga
     2041 caaaaagaaa aacaagaaaa agaaaaagga ctgctgcgca aacaaccaga acggcgccac
     2101 ctgcacccac gacggcaaag gaaattgctg caaggacaag gacaaatgcc aggacaagga
     2161 tatgaacaag cagcaggacg acagcaagaa agatccgaag taatttttcc tctttcagga
     2221 aaagatcctc atccctgtga tggggatttt ttttgcagca tatcctgcag ctccttcagc
     2281 ctgcggatct cgtacgtcac tttctccttg tgctgtccgc cggaaggatt gaaaaaaacc
     2341 tggtgccagc cggcttcacg cgcacccacg atatcgatgt tcaggtcgtc gccgatcatg
     2401 agcgattctt cggcaacagc tttcgctttt ttcagcgcgt aggaaaaaat acgcgcatcc
     2461 ggcttggcat agccgcagcc ttcggaagtg atgatcgttt cgaaatagga ttcgagcccg
     2521 ctgcgcgaca gcttgtgatg ctgcacttcg ggaaaaccgt tggtgatgat gtgcaggcgg
     2581 taatcctcct taatgctttc gagcaattct attgtacccg gaataagatt ttttttgttc
     2641 ggcgcttctg aaatataatc gcggtcgaaa ttcgccgcga gctcttcgtc gtgcacaccg
     2701 aaatgcgcga ggctctgctt gaagcgcagc gcgcgcagcg ttgctttatc gatgatccgt
     2761 ttctggtaac ggtcccagag aagatggttg tagtggttgt aatgatccac gaaatcttcc
     2821 gccgaatcga tccccctcga agcaagatcg tacttgtggt acagctcgac cagcgtttca
     2881 cgtgcgttcc tttccatgtc ccacagcgtg cggtcgagat cgaaaaagag atgtttatat
     2941 accgccacgt cagttcgcct ggtaaggaat gtattgagaa agctgcagga tctgcgcaag
     3001 tgcattcagc acgagcgacc agaacagcag cgacgcgatg aggtaaagca gcacgcgttt
     3061 tcttttccgg aaaaagtgag ccgccacgat cgtcccgatg ggaatggaaa tgatggcggg
     3121 tgtaattacc gctattcccg tcagcccgaa tttcattttc acgccgatga taaaacgccg
     3181 tgtgcgtgta aaagatctcg ggggtttgcg ttcgataacg gaggcgggac gacgcaggaa
     3241 taccgacttg atgattgaaa cgacatgccg ccaccaggtg gtgatgttcg aacctgcgta
     3301 ataaaatgca agaataccta caaggccgcc gaacgtggtt acaccgaacg cctcgccgaa
     3361 tcccatgtga taacgctcgg cttcgaatgg tgcgaaaaga aatttgacgc ttgcgatcag
     3421 ggcgacttca agaacctcca gccaatgctt gacctgattc gcgtccatag aggatcagag
     3481 tttttccccg aaggcgagat cgcccgcatc accaagcccc ggaacgatgt atgactgcgc
     3541 agtaagctca tcatccaccg cgcaggtcca gatggtgacg ttctcgggaa gattcttcct
     3601 gagataatcg atgccgaaaa gactggagat cacagaaacc gcgtgaatat gccggggttt
     3661 tccgtggcgc aggatcgcct ggtacgcaag caccatcgaa gtcccggtgg cgagcatagg
     3721 atcacagagg atcagctctt ttccttcgac agacgggctg gcgaggtatt ccacgtgaac
     3781 gtcgaaagag ccgtccttgt ggtgccgccg gtaagctgaa ataaatgcgt tctccgcctt
     3841 atcgagaatg ctgaggatcc cgtaatgcag cgggataccc gcccgcagaa tcgtagccac
     3901 caccggctgc tctttgagca cgggcacctg ggcgatgccg agcggcgtcg ttacttccgt
     3961 ggtcacgtat tcaagctttt tgctgatctc gtacccgatg atcttaccga tgcgttcgat
     4021 gttcaggcga aaccgcatgc tgtctttctg gacagaaaca tcgcgaagct cggccatgta
     4081 attattcagg acagaagatt gcttgctcag gtcgtttatc ataaaccgga ttttgtgggt
     4141 cgtgcttatc gcttgtacca gcgaatatac cacgggaggc ggttgcagcg gacttgcaaa
     4201 tatacgcatt ctttag
//
LOCUS       DASTSF010000588         9047 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_734046,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000588 DASTSF010000000
VERSION     DASTSF010000588.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 9047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9047
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_734046"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..951)
                     /locus_tag="VFU15_16605"
     CDS             complement(<1..951)
                     /locus_tag="VFU15_16605"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009284168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEU4719467.1"
                     /translation="MKTVDDFKFSGKKVLVRVDFNVPLNEKFVITDDTRMRAAVPTIK
                     KILADGGSCILMSHLGRPKEGPSEKFSLKHLVAHLSGLLGGVKVHFAGDCIGEPAKTA
                     AAALKPGEVLLLENLRFHKEEEKGDENFAKELSSLGDFYVNDAFGTAHRAHASTAVIA
                     KFFPGSKCFGYVMSGELASVSKVLTGTVRPFTAVMGGAKVSDKILILEQLVGKADHII
                     IGGGMAFTFLKAMGGNVGKSLVEEDRIANAIKILREAEHRGVKIHLPVDAVCADNFSN
                     DANRKECSATEIPDGWMGLDIGAATEKRYADVIMLSKTILW"
     gene            1105..1728
                     /locus_tag="VFU15_16610"
     CDS             1105..1728
                     /locus_tag="VFU15_16610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719468.1"
                     /translation="MTPQKLVSLFALCCVCVMTKAQDTISLCKGKMAEVMVEKCVYEN
                     SATDFFYLHVILKNLTDDTIGVDLSHNEDVVHINQWTTGDTTFRMVIDEAELTYSDPD
                     RMREQQLKNDFHAGKLTIIPPHSSVDYYHNFNGGPAKNLRRQEHEDKSKYVLFSLKGQ
                     LTFTDGKTLEDFHTGDYEHGKDNFPDVAIRCPVEWKKIPAGALIITD"
     gene            1791..2264
                     /locus_tag="VFU15_16615"
     CDS             1791..2264
                     /locus_tag="VFU15_16615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719469.1"
                     /translation="MMRGFFIASILLSLAYFIAAMITIAGITEARRTGTYETQRRYME
                     NDGSPGTYGGIPSSYEDVAQEETWCAVVISVVYFLLMEAVFLAGLVKIKTQTMKIFSI
                     IGMSLAGFFLACSFLPLNNPVSEPFDQVVPALMIASVVFLAFSIIGTVHAFKTKT"
     gene            2291..3127
                     /locus_tag="VFU15_16620"
     CDS             2291..3127
                     /locus_tag="VFU15_16620"
                     /inference="COORDINATES: protein motif:HMM:NF044571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719470.1"
                     /translation="MKPVFSASVVFFVLLNFYSCRQDNPSPPVNVNPQNNTYTPVNHG
                     PEHAVENSSHSVIAYVDSDGTYLNSSRSTIGHFAADGAVENDSYGTVGYIASGGVIEN
                     SSHSTIGYVQANGAVENSSHGTIGYVQANGTVENSSHGTIGYAVNVDVKQAAVIFFFN
                     NTLFQAAFISNVRYILSDGTIENESYSTIGHILSDGTVENASYSTIGHLQNDGTIENG
                     SYSTIGHVQSDGTVENASYSTIGHIASDGTVEDEHYTTIAHFPGVDAKWISVIFFFDF
                     QP"
     gene            3124..3807
                     /locus_tag="VFU15_16625"
     CDS             3124..3807
                     /locus_tag="VFU15_16625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719471.1"
                     /translation="MSRAVTFAQFFSAALLLLSCGKTINAPPAIKSIVPAKVPGDYLL
                     VCKNSMGETYLDQSLFSDTNDSLVYLHFAIRNTTADTIGTSFDASGKFGVYPNTWGFC
                     DTGAPVICWDECVELLEPLTKAAILNLENQFAAGRLKNIPPGQMLDYYYCIDTKQDLM
                     SQLGKSNLKTPRLRFTFGGRCMLTTRKITDDSFYGDSRKGATGFPMLNTNRPLSFRRL
                     PGKAFIYTR"
     gene            3890..4168
                     /locus_tag="VFU15_16630"
     CDS             3890..4168
                     /locus_tag="VFU15_16630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020597898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autorepressor SdpR family transcription factor"
                     /protein_id="HEU4719472.1"
                     /translation="MNALFKALDDPTRREILELLKKKDMTAGEIADAFTISKPSISHH
                     LDLLRQANLVISIKEGQFIRYSLNATVFDELLSWVMNMTKKKKKTPKK"
     gene            4172..4837
                     /locus_tag="VFU15_16635"
     CDS             4172..4837
                     /locus_tag="VFU15_16635"
                     /inference="COORDINATES: protein motif:HMM:NF025015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HEU4719473.1"
                     /translation="MKTSGFISREWILWLLILAPLTLVLARWSDYPAKIPTHWNAAGE
                     VDDYGGKGALFLSPLINTGLYLLLLLLPKIDPRKKNYNLFGGAYWIIRIALAFLLSGI
                     GFVTCLASLGYRIDVGTIVPIAVFALFMIMGNQFARIRSNYFVGLRTPWTLNSEEVWT
                     RTHRVTGRIWVAASAIMIVLSFFLKGETLFVVMIPYIVILSVFPFVYSWKLWKDSQQN
                     EVK"
     gene            complement(4881..5936)
                     /locus_tag="VFU15_16640"
     CDS             complement(4881..5936)
                     /locus_tag="VFU15_16640"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HEU4719474.1"
                     /translation="MKTNFIKPIVILASALIVNTIAAQDIHFSQFYMSPLTVNPALAG
                     AQHDMTVAINYKDQWQSVASPYKTVAASWDMRMMQSKNKKGFAAVGVNFFSDRSGDAK
                     LGTTQGNATVAYHVRLNSNNTFGGGVQAGFAQRSISFGSLTWGNQFDGLAYNATLPSG
                     EPAAGATVSYADFGGGLDWNFDNRSGAKNVTDNHDQKINFGIALYHIGEPKYAFYSAS
                     QEKLFMRYSVHGSALLSVPNSNIAFAPAFMYEMQGPSKELYAGSLLRFSLSQDSKYTG
                     FKQGAAISFGGFLRAKDAVAAVMLLEYSHYALGISYDVNVSGLKTASNSRGGIEIALR
                     FVSPNPFQPSSSSSFFN"
     gene            complement(5961..>9047)
                     /locus_tag="VFU15_16645"
     CDS             complement(5961..>9047)
                     /locus_tag="VFU15_16645"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HEU4719475.1"
                     /translation="PYTCTVTDVNGCTTVQSVTITQPAALTVSLSSQINVTCNGGNNG
                     EATVTAGGGSPGYSYAWSPSGGNASTAPNLISGNYTCTVTDNNTCTVTQTVNITQPPA
                     ITSTVTSVNSTCGNSNGTATVAASGGAGSFSYLWTTTGDTMATDTALAAGTYSVDITD
                     ANGCTSGGTVTVSNTPGPAVSLASQVDVTCNGGNNGTATVNPSGGTLPYSYSWSPSGG
                     NAITGTSLAAGTYTCTITDANNCAVTQTATITEPLAVTATTSTVNATCGNSNGSATVN
                     ASGGSGGYTYSWAPSGGNAATESNIPAGTYTCTITDVNGCTGTSIVSVNNIGGPVVSL
                     ASQIDVTCNGGNNGTAMVNASGGNPGYTYAWNPSGGNAATGTSLSAGTYTCTVTDASG
                     CIQTQTVQITEPPVITTSSVFTPETCGNGNGTATVTAGGGAGSFTYSWSPSGGNAATA
                     SGLSAGNYSCTVTDANGCTATETVNIVNSAAPVVTLASQTDVSCAGGTNGQAMMNVSG
                     GAPGYTYVWSPSGGNTATGTSLTAGTYTCDVTDANGCASSQTVTITEPAPITATMSVV
                     NAACSQSNGSATIAVSGGTGSYSYSWSPSGGNASTANALSAGTYVCIVTDANGCSHLD
                     TAIVNTTNGPSVTVTSQTDPTCNGLTDGSVTVNVTGGTPGYTYSWSPSGGTTATGSGL
                     GQGSYTCTITDAAGCVQTQTATLTEPALLTATSSFTNVLCNGGTTGTASVIAGGGTPN
                     YSYNWSPTGGTSSNATGLAQGSYTCTITDANGCTATQSVTITQPSAITVSATPSAGCG
                     VNGGSAVATAGGGTPGYSYAWTPSGGTSASATGLAAGSYTCTVTDANGCSANTPVTVT
                     TNPLPTPTVSSGTTIIIGSSTTLTATGGTSYVWTPSTDLSCVSCQSPVASPTQTTTYC
                     VNVTDANGCSDSACTTVAVNTDCGTIFVPSGFSPNGDNENDQLCIYGTQCMQSVDFVI
                     YDRWGEKVFETTDPHLCWDGTYKGEMLNNGVFVYYLQAYMLDGSIKTQKGNITLVR"
BASE COUNT         1968 a         2526 c         2426 g         2127 t
ORIGIN      
        1 ccagagaatt gttttcgaca gcatgatgac atccgcatag cgtttttccg ttgccgcgcc
       61 gatatcgagt cccatccagc cgtccgggat ttccgttgca ctgcattctt tccggttcgc
      121 gtcgttggaa aaattatccg cgcacaccgc atccacgggc aggtgaattt tcacgccacg
      181 gtgttccgct tcccggagta tcttgatcgc gttcgcaatg cggtcttcct ccaccagcga
      241 tttgccgaca ttgccgccca tcgctttcag gaaggtgaac gccattccgc cgccgatgat
      301 gatatgatcg gctttgccga cgagctgctc gagaatgaga attttatccg acactttcgc
      361 gccgcccatc accgccgtaa aagggcgtac ggtgccggtg agcactttgc ttacgctggc
      421 gagttcgcct gacatgacat agccgaaaca tttgctgccg ggaaaaaatt tcgcgataac
      481 agccgtggat gcatgcgctc ggtgagccgt cccgaacgca tcattgacgt aaaaatcgcc
      541 gagcgaggaa agctctttcg caaaattctc atcgcctttt tcctcttcct tgtggaagcg
      601 gagattttcc agcagcagca cttcgcccgg cttgagcgcg gcggcagctg tttttgccgg
      661 ttcaccgatg cagtcgccgg cgaaatgcac tttcacacca ccgagcaacc ctgaaaggtg
      721 cgcgacaagg tgtttcagcg aaaatttttc cgacggtcct tctttcggcc ggccgaggtg
      781 ggacatgagg atgcacgaac ctccgtcggc gagaattttt tttatcgtgg gaacggcagc
      841 gcgcatacgc gtatcgtccg ttatcacgaa tttttcattc agcggaacgt tgaagtcaac
      901 ccgcaccagg acctttttac cggaaaattt gaagtcgtcg actgttttca tcatgtgaac
      961 ggaaagttaa cggcaaaaat aatattattg acagtaaaga tgcgatccgc ccgttattcc
     1021 ggccggcagc tcactcccgg ggagtttgtg tgtttcttgt gtcctgttcc gcagatgcgt
     1081 acttttaatt attaccgatc tccgatgacc ccgcaaaaac tggtcagcct gtttgccctc
     1141 tgctgcgttt gcgtgatgac gaaagcgcag gatacgatct cactctgcaa gggaaaaatg
     1201 gccgaagtga tggtggaaaa atgcgtgtat gaaaactccg ccaccgattt cttttacctg
     1261 cacgtcatcc tgaaaaacct gacggacgac acgatcggcg tcgatctttc gcacaatgaa
     1321 gacgtcgttc acatcaacca gtggacgacc ggcgatacga cgttccgcat ggtgatcgat
     1381 gaggcagagc tgacctattc cgatccggac cgcatgcggg aacagcagct gaaaaatgat
     1441 tttcatgcgg gaaagctcac gatcatcccg ccgcattcgt cggtggatta ttaccacaac
     1501 ttcaacggcg gacctgcaaa aaacctgcgc aggcaggagc acgaagacaa atcaaagtac
     1561 gttctcttct cgctgaaagg acagctgaca ttcaccgacg gaaaaacgct cgaagatttt
     1621 cacaccggtg attacgaaca tggcaaggac aattttccgg atgtggccat ccgctgtccc
     1681 gttgaatgga aaaaaatccc cgccggtgcg ctcatcatta ccgactgaaa agaatgcgcc
     1741 ccgctctcct ctcccttttc ctatattagc cttgatcaac ctcatcgccc atgatgcgcg
     1801 gatttttcat tgcgtccatc cttctttcgc ttgcctattt cattgcagcc atgatcacca
     1861 tcgcaggcat tacggaagca cgcaggaccg gcacctatga aacgcagcga cgctatatgg
     1921 aaaacgacgg ttccccgggc acgtacggcg gcatcccctc ctcttacgaa gatgtagcgc
     1981 aggaggaaac atggtgtgcc gtagttatca gcgtcgttta tttcctgctc atggaagctg
     2041 ttttcctggc gggtcttgta aaaatcaaaa cgcagaccat gaagatattt tcgatcatcg
     2101 gcatgagcct ggccgggttc ttccttgcct gttcattcct tccgctgaat aatcccgtaa
     2161 gtgagccgtt cgaccaggtg gtccctgcct taatgatcgc cagtgttgtg ttcctcgctt
     2221 tttccatcat cggaacggtt cacgcgttca aaacaaaaac ataactccct taccgtttta
     2281 ccccgctgaa atgaaacctg ttttttccgc aagcgtcgtt tttttcgtgc tgctgaattt
     2341 ttattcctgc cggcaggata acccgtcccc gccggtgaac gtcaatccgc agaacaatac
     2401 ttacacgccg gtaaatcacg gacccgaaca cgcggtcgag aacagctcgc acagcgttat
     2461 tgcctatgtc gacagtgacg gaacgtacct gaattcctcg cgcagcacga tcggtcattt
     2521 tgcggcggac ggtgcggtcg aaaatgattc atacggcacg gtcgggtaca tcgcttccgg
     2581 cggcgtgatc gaaaactcgt cgcacagcac gatcgggtac gtgcaggcaa acggcgcagt
     2641 ggaaaattca tcgcacggca cgatcggcta cgtgcaggca aacggcaccg tggaaaattc
     2701 atcgcacggc acgatcggtt atgccgtgaa cgtcgatgtg aaacaggcgg ccgtgatctt
     2761 cttcttcaac aatacgctct tccaggccgc gttcatttca aacgtgcgtt acatcctcag
     2821 cgacgggacg atcgaaaatg aaagctactc cacgatcggc catatcctca gcgacggaac
     2881 cgtggaaaat gcaagctatt ccacgatagg ccatctgcaa aacgacggaa cgatcgagaa
     2941 cggaagctat tccaccatag ggcatgtgca gagcgacgga accgtggaga acgccagcta
     3001 ctcgacgatc gggcacatcg cttccgacgg aaccgtggaa gacgaacatt acacgacgat
     3061 cgcgcatttc cccggcgtcg acgcgaagtg gatctcggtc attttctttt tcgattttca
     3121 gccatgagca gagccgtaac ttttgcgcag tttttttcag ccgcgctgct gctcctgtcg
     3181 tgcgggaaaa cgattaacgc accgccggcg ataaaatcaa ttgtccccgc aaaagtgccc
     3241 ggcgattacc tgctggtctg caaaaattcc atgggcgaaa cctatctcga ccagtcgctg
     3301 ttttccgata caaatgattc cctcgtttac ctccatttcg cgatccgcaa tacgacggcc
     3361 gatacgatcg gcacgtcttt cgatgccagc gggaaattcg gcgtttatcc caacacgtgg
     3421 ggattctgcg acacgggtgc gcccgttatt tgctgggatg aatgcgtcga gctgctcgaa
     3481 ccgcttacga aagccgccat cctcaacctg gaaaaccagt tcgcggccgg caggctgaaa
     3541 aatatcccgc ccgggcagat gctggattat tattactgca tcgacacgaa acaggatctt
     3601 atgtcgcagc tcgggaaaag caacttaaaa acgccgcgcc tgcgtttcac gttcggcggt
     3661 cgctgcatgc tgacgacccg taagatcacg gacgacagct tttacgggga cagccgcaag
     3721 ggcgccaccg gctttcccat gctgaacacg aaccggccgc tttctttccg ccgtcttccg
     3781 gggaaggctt tcatctacac ccgctgaatt tcctcctgtg ttccaggctt ttgcataaat
     3841 tagtatttag atatttgtct aattatacaa ttatgcatac gtttgtccca tgaacgcatt
     3901 attcaaagcg ctggacgacc cgacgagaag ggagatcctc gaactgctga aaaagaaaga
     3961 catgaccgcg ggcgagatcg ccgacgcgtt cacgatcagc aagccgagca tctcgcacca
     4021 cctcgacctg cttcgccagg cgaacctggt catttccatc aaggaagggc aattcatccg
     4081 ctactcgctg aacgcaaccg tgttcgacga gctgctctcc tgggtgatga acatgacgaa
     4141 aaaaaagaaa aaaacaccta aaaaataaac tatgaaaact tccggcttta tcagcagaga
     4201 atggatactc tggcttctca tcctcgcccc gctcacgctt gtgcttgcac gatggagcga
     4261 ttaccccgca aagatcccga cgcactggaa tgccgccggc gaggtggacg attacggcgg
     4321 gaaaggagcg cttttcctca gcccgctgat caacacgggc ctttatttac tcctgctcct
     4381 tcttccgaag atcgatccgc ggaaaaagaa ttacaacctg ttcggcggcg cctactggat
     4441 catccgcatt gcgcttgcgt tcctgctttc gggaatcggc ttcgtcacgt gcctggcttc
     4501 acttggttac aggatcgacg tgggcacgat cgttcccatc gcggtgttcg cgctgttcat
     4561 gatcatgggc aaccagttcg cgagaatacg ttcgaactat ttcgtgggac tgcgcacgcc
     4621 gtggacgctg aacagcgaag aagtatggac gcgcacacac cgtgtgaccg gccgtatctg
     4681 ggtggcggcc tctgccatca tgatcgtgct ttcctttttt ctgaaaggcg aaacgctgtt
     4741 cgtcgtcatg atcccctaca tcgtgatcct ctcggttttc ccgttcgtgt attcgtggaa
     4801 gctctggaaa gattcacagc agaacgaagt gaaataaaga atccggccgt aactggccgg
     4861 attcttttcc gctaaatttt tcagttgaaa aagctgctcg aagaagaagg ctggaacggg
     4921 ttcggcgaga caaagcgcag cgcgatctcg ataccgccgc ggctgttgct tgctgttttc
     4981 agtccggaca cgttcacgtc gtagctgatg ccgagcgcat aatgtgaata ctcgagcagc
     5041 atgaccgcag ccacggcatc ttttgcacgg agaaatccgc cgaaagaaat cgccgctccc
     5101 tgtttgaagc ccgtgtattt cgaatcctgg ctcagcgaaa aacgaagaag cgatcctgca
     5161 taaagttctt tcgagggacc ctgcatttcg tacatgaatg cgggagcaaa agcgatgttt
     5221 gaattcggca cgctgagcag ggcggaaccg tgcacgctgt accgcatgaa aagcttttcc
     5281 tgcgatgcgc tgtagaaagc atatttcggt tcaccgatat gatacagggc gatgccgaaa
     5341 tttattttct ggtcgtggtt atcggtcacg ttctttgccc cggagcggtt atcgaaattc
     5401 cagtcgaggc cgccgccgaa atccgcgtac gaaaccgttg cgcccgctgc aggttcaccg
     5461 ctcggcagcg tagcgttata ggcgagaccg tcgaactggt ttccccacgt gagcgagccg
     5521 aacgaaatgc ttcgctgcgc aaagccggcc tgcacgccgc cgccgaacgt gttgttcgaa
     5581 ttcaggcgaa cgtgatacgc cacggtggcg tttccctgcg tcgtgcccag ctttgcatcg
     5641 ccggaacggt cgctgaaaaa attcacgccg acagcggcaa atcctttttt gtttttgctc
     5701 tgcatcatcc gcatgtccca gctggccgcc actgttttgt acggactggc cacgctctgc
     5761 cattggtctt tgtagttgat cgcaacggtc atgtcgtgct gcgcaccggc cagtgcggga
     5821 ttaacggtca gcggcgacat gtagaattgc gagaagtgaa tgtcctgcgc ggcgatggtg
     5881 tttacaatca atgccgaagc aagaatgacg atgggtttta tgaagttcgt tttcatggct
     5941 ttttcttttc cggttgatga ttatcgcacg agcgtgatgt ttcctttctg tgttttgatg
     6001 gagccgtcga gcatgtatgc ctgcaggtaa tacacgaaca cgccgttgtt cagcatttca
     6061 cccttgtacg tcccgtccca gcacaaatgc ggatccgttg tctcgaagac tttctcgccc
     6121 catcggtcgt agatcacgaa gtcaacggac tgcatgcatt gcgttccgta aatgcagagc
     6181 tggtcatttt cattatctcc gttcggggaa aatcctgacg gaacgaaaat ggttccgcaa
     6241 tccgtattca ccgcaacggt cgtgcaggct gaatccgaac atccgttcgc atcggtcaca
     6301 ttcacacaat aggtcgtcgt ctgtgtgggg ctggccaccg gcgactggca ggagacgcag
     6361 ctgaggtcgg tggaaggcgt ccacacgtag cttgttcctc ctgttgctgt caacgtggtg
     6421 gatgagccga tgatgatcgt ggttccggaa gaaaccgttg gcgtgggcag cgggttcgtg
     6481 gtaacagtca ccggtgtatt cgcgctgcag ccgttcgcgt ctgtgacggt acacgtatag
     6541 cttcctgcgg caagtcccgt tgcggaagcc gacgttccgc ccgaaggcgt ccacgcatac
     6601 gaatagccgg gcgtgccacc accggcagtt gcgactgcag aacctccgtt cacgccgcat
     6661 ccggcggaag gcgttgcgga aaccgtgatc gcagatggct gcgtgatcgt cacggattgt
     6721 gtcgcagtgc aaccattcgc atcagtgatc gtgcacgtat aggatccctg cgcaagaccg
     6781 gttgcattcg aagatgttcc gccggtaggc gaccagttgt atgaatagtt cggtgtgccg
     6841 ccgccggcaa tgacggaagc tgtcccggtt gttcctccat tgcacagcac gttcgtgaat
     6901 gaagatgtcg cggtgagcaa tgcaggttcc gtcaatgttg cagtttgcgt ctggacacaa
     6961 cccgccgcat cggtgatcgt gcaggtgtag gatccctgcc cgagaccgga tcccgttgcc
     7021 gtcgttcccc cggaaggact ccacgaatac gtgtagccgg gcgtgccgcc agtgacgttc
     7081 actgtaacgc tgccatcggt aagaccgttg catgtcgggt cggtctgcga cgtgacggtt
     7141 accgaaggcc cgttcgtcgt gttcacgatc gcggtatcga gatgcgagca gccattggcg
     7201 tcagtaacga tacaaacata agttcccgcg ctcagcgcat tcgccgttga agcatttcca
     7261 cccgaaggcg accaggaata agaatacgat cccgttccgc cgcttactgc gatcgttgct
     7321 gatccgttgc tctgtgagca ggcggcatta acgacactca tcgtcgcagt gatcggggcc
     7381 ggttcggtga tcgtcactgt ttgcgaagaa gcacaaccgt tggcgtccgt aacgtcacac
     7441 gtataggttc ctgcagtcag cgaggttccc gttgctgtat ttccaccgga aggactccac
     7501 acgtaagtat agccgggcgc accaccgctc acgttcatca tcgcctgccc gttcgtaccg
     7561 cctgcgcagg aaacatccgt ctgcgaagca agtgtcacga ccggcgccgc cgagttgacg
     7621 atgttcaccg tttccgttgc agtgcagccg ttcgcatccg taactgtgca ggaataattt
     7681 cctgcgctga gtccggaagc cgttgccgca ttgccacccg agggcgacca gctgtacgtg
     7741 aacgagcctg ccccgccccc ggctgtaacc gttgccgtac cgttaccgtt gccgcatgtt
     7801 tcgggtgtga agacagatga tgtcgtgatg accggaggtt cggtgatctg caccgtttgt
     7861 gtttgaatac aacctgaagc atcggtcacc gtgcaggtat aggttccggc agaaagcgat
     7921 gttcccgttg cggcattgcc accggaagga ttccatgcgt atgtgtagcc cgggtttccg
     7981 ccgcttgcat ttaccattgc cgttccgtta ttgccgccgt tgcacgtaac atcgatctgc
     8041 gacgcaagcg aaacaaccgg cccgcctata ttgttcacgg aaacaatgga tgtccccgtg
     8101 cagccattca catcagtgat cgtgcatgta taagttccgg ccgggatgtt ggattctgtc
     8161 gcagcgttgc cgccggaagg agcccacgaa taggtgtatc cgccgctgcc gccgcttgca
     8221 ttcaccgttg cggaaccatt gctgttgccg catgttgcat tgaccgtgga agtagttgct
     8281 gtaacagcaa gcggttcggt gatcgtcgct gtttgcgtta cggcgcagtt gttcgcgtcc
     8341 gtgatcgtgc aggtgtatgt tcccgctgca agagaagttc ccgtgatggc atttccgccg
     8401 gacgggctcc acgaatacga atacggaagc gtgccgccgg aaggatttac cgttgccgtt
     8461 ccgttattgc cgccgttaca cgtaacgtcc acctgcgaag caagagaaac ggcaggtccc
     8521 ggggtgtttg agaccgtcac ggttccgccc gacgtgcatc cattcgcgtc ggtgatgtcg
     8581 acagaatatg ttccggccgc aagagccgta tccgtcgcca tcgtgtcacc ggttgtcgtc
     8641 cagaggtaag agaatgatcc cgctcccccg gacgccgcaa cggttgccgt tccgttgctg
     8701 ttgccgcatg tcgagttcac gctggtgacg gtggatgtga tcgcaggcgg ctgtgtgatg
     8761 ttcacggtct gcgtaacggt acacgtgttg ttgtctgtga ccgtgcaggt gtaatttccc
     8821 gaaataagat tgggcgcggt tgatgcgttt ccgccggaag gtgaccatgc atacgaatac
     8881 cccggcgatc cacctcccgc tgtcaccgtc gcttcaccgt tatttccgcc attgcacgta
     8941 acgttgatct gtgatgaaag agatactgta agcgcagccg gttgcgtgat cgtgaccgac
     9001 tgaaccgtgg tgcagccgtt cacatcggta acggtgcatg tataggg
//
LOCUS       DASTSF010000589         4615 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1702695,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000589 DASTSF010000000
VERSION     DASTSF010000589.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4615
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1702695"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1648)
                     /locus_tag="VFU15_16650"
     CDS             complement(<1..1648)
                     /locus_tag="VFU15_16650"
                     /inference="COORDINATES: protein motif:HMM:NF027900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5060 domain-containing protein"
                     /protein_id="HEU4719476.1"
                     /translation="MRALLFYVLFLLPVVLCARETYETRIISGGGNEVKQYYTIEIGI
                     RAPFADRMFTAFGVNPYEFRNFPLQAEFTCNGKSFRSPVFYFEETHADEKANRYFSEP
                     SEWPWRVRFSPPDTGNWQMTLLAGIEPMTVPMSTGILFHCTKSDRHGWLQVADDHRHF
                     CYADGRPFFVLGQNICWADEPVLKGHSIAPTLYNNGYYDVYHYLGNLADNGGNYVRIV
                     MAPWSTGIEWEETGRYSQERAFALDSMLKIAEEKNLQVQLCLDLTTGFAADNDSNTYH
                     PYRKSFQEPGMTASDLLTDSSALHAFDNFIRYVCARWAYSPNVCCLELMGEQDRWQGY
                     AGKEKNFDDFFVRVNRFVRDSVHNAHVMITTSMTGPKRIATFRNPAIAIVDIHNYDND
                     LRCNVKRFALMHRSDIRKCDKPFLFGEMGMIAGPVNACDPNDYERCSDISMHNAMWAT
                     TFMGGAGAGLYWWQWGNDGYREANFPALRWFIDSVAGGMSGYTGYGMWSGNGIETFYS
                     GNKRSCVGWAHNTSYWWCNMTDSCRDRNGKEMIHPKDDDKA"
     gene            1676..2317
                     /locus_tag="VFU15_16655"
     CDS             1676..2317
                     /locus_tag="VFU15_16655"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HEU4719477.1"
                     /translation="MSAGKKTKTVFSKAERSHPLKMLVYFVLAGISMLFFTLMTLFAF
                     SGPERHLGGQKFPKYFLVSTVIILLSSFFLESARRAFREENGKKLLDRMMITMVLALA
                     FSVAQVMGWMQLWDSGITLYGIGGIPPTTGTPSGAFLFLISGLHLLHLSVGLVFLFLT
                     MFKVVHTRSDDVRSVIFFTNRLERTRIDMLTLYWHFLGGLWLILFLYFLWFFV"
     gene            2432..3817
                     /locus_tag="VFU15_16660"
     CDS             2432..3817
                     /locus_tag="VFU15_16660"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEU4719478.1"
                     /translation="MENPENRFYLDQWHQEVRRLMIKSSSASWPLTSLCLAAFIFVEI
                     YHRNQPDYITPFYFLFLGCSVLVMSAHLLRKKIKYPYIINAFGTSILVPIVTTIAACI
                     TVSENVFTYFLMGSVVIVVRGLLYCQKVKTLAIIAVCSHVLIFTTVLLVRKEPYLSLP
                     NILSTNIIQLFLVMFSLSGTASRYKLTRDNFLNSLRLADVNKTIAEKNKDITDSINYA
                     RRIQRSLLPQQHFLDSNIGEHCVFYRPKDIVSGDFYWASRLQNGDFAIVVGDSTGHGV
                     PGAIMSMLNIASLNEIQNHTFITSPASVLDHARKRIIEHLDNHENEEGAKDGMDAIVV
                     CIDKEFKKLTYAAANNSLWLVRNGEVDQLKGDKMSVGKDANVRHAFTERTIALQKNDL
                     LFMFTDGYTDQFGGARGKKLMIKRFRETMRNIAHLPLQEMREKLADHFDEWKGEHEQT
                     DDVLVFAMRVN"
     gene            complement(3856..4038)
                     /locus_tag="VFU15_16665"
     CDS             complement(3856..4038)
                     /locus_tag="VFU15_16665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719479.1"
                     /translation="MHAFANKTDETIEFLCVANPGVFGPDFFKDVAEVINAGGPPDMA
                     KLKQAMLHHGLVPVAG"
     gene            complement(4298..>4615)
                     /locus_tag="VFU15_16670"
     CDS             complement(4298..>4615)
                     /locus_tag="VFU15_16670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719480.1"
                     /translation="TLGSLGTKQNLYLTKGGYAILQFNIQDDIFLQVYTPYDTLKYKF
                     SDFERDTRLTDRAFMDDLFKLKITGTGREIVTIRNSERYINVANPQHIYPGIRVEYEW
                     PGK"
BASE COUNT         1080 a         1242 c         1178 g         1115 t
ORIGIN      
        1 ccgccttgtc gtcgtctttc gggtggatca tttcttttcc gttccggtcg cggcaggagt
       61 ccgtcatgtt gcaccaccag tagctcgtgt tatgcgccca tcccacgcag ctccgtttgt
      121 tcccgctgta aaatgtttcg atgccgtttc ccgaccacat gccgtaaccg gtatagccgc
      181 tcatcccgcc cgccaccgaa tcgatgaacc agcgcagcgc cgggaaattg gcttcccggt
      241 agccgtcgtt tccccattgc caccagtaaa gtccggcccc ggccccgccc atgaacgtcg
      301 tcgcccacat cgcgttgtgc atgctgatat cgctgcaccg ttcgtaatcg ttcgggtcgc
      361 aggcattcac cggcccggcg atcatgccca tttcaccgaa gagaaaaggt ttatcgcatt
      421 tccggatatc gctgcggtgc atcaatgcaa aacgcttcac attgcagcgc agatcattgt
      481 cgtaattgtg aatgtcgacg atggcgatgg cgggattccg gaaggtagcg atgcgtttcg
      541 ggcctgtcat gctcgtggtg atcatcacgt gtgcattgtg aacggagtcg cgcacaaacc
      601 ggttcacacg cacaaaaaaa tcatcgaagt ttttttcttt tcctgcgtaa ccctgccagc
      661 ggtcctgctc gcccatgagc tcgaggcagc atacgttcgg cgaatacgcc caccgtgcgc
      721 agacataacg gatgaaatta tcgaacgcat gcaatgccga ggaatccgtc agcaggtcgc
      781 ttgcggtcat gcccggttcc tggaatgatt tccggtacgg atgatatgtg ttcgaatcat
      841 tgtccgcagc aaaaccggtg gtgagatcga ggcaaagctg cacctgcagg tttttctcct
      901 cggcgatctt cagcatgctg tcgagcgcga atgcgcgttc ctgcgaataa cgtcccgttt
      961 cttcccattc gatgcctgtg ctccaaggcg ccatgacgat gcggacataa tttcctccgt
     1021 tgtccgccag gtttccgagg taatgataaa catcgtaata accgttgttg tacagcgtgg
     1081 gcgcaatgga atgacctttc agcacgggct cgtccgccca gcagatgttt tgccccagca
     1141 cgaaaaaagg gcggccatcg gcatagcaga aatgccggtg atcgtccgcc acctgcagcc
     1201 atccgtgacg atcgcttttt gtgcaatgaa aaaggatccc ggtcgacatg ggaacggtca
     1261 tcggctcaat gccggcgaga agcgtcatct gccagttgcc cgtatccggc ggtgaaaaac
     1321 gcacacgcca gggccattcg gagggttcgg aaaaatagcg gttggctttt tcatccgcat
     1381 gcgtttcttc gaaataaaag acaggcgaac gaaagctttt cccgttgcat gtgaattcgg
     1441 cctgcagcgg gaaattgcgg aactcgtagg ggttgacgcc gaaagcggtg aacatcctgt
     1501 cggcgaacgg cgcgcgtatt ccaatttcga ttgtgtaata ttgcttcact tcgtttccgc
     1561 cgccggaaat gatgcgcgtc tcgtacgttt cccttgcaca aagcacaacc ggcaacagga
     1621 agaggacgta aaacagcaaa gcgcgcatgg tccaaagttg gcaaaaaaca acagcatgag
     1681 tgcgggaaaa aaaacaaaaa ccgtcttttc aaaagcggaa cgcagccatc cgctgaagat
     1741 gctggtgtat ttcgtactgg cgggcatctc catgcttttc ttcacactca tgacgctctt
     1801 cgcgttcagc ggcccggagc ggcatctcgg cggacagaaa ttcccgaaat attttttagt
     1861 aagcacggtt atcattctgc tcagcagttt cttcctcgaa tcggcgagac gtgcgttccg
     1921 cgaggagaac ggcaaaaagc tgctcgaccg gatgatgatc acgatggtgc tggcgctggc
     1981 gttttccgtt gcgcaggtga tgggctggat gcagctgtgg gattcgggca ttacgctgta
     2041 cggcatcggc ggaattccgc ccaccacggg aacaccgagc ggtgcattcc tttttctcat
     2101 ttcgggattg cacctgctgc atctttccgt cggactggtg tttcttttcc tgacgatgtt
     2161 caaggtcgtc cacacgcgca gcgacgatgt gcgcagcgtg atcttcttca ccaaccgcct
     2221 cgagcgcaca cgcatcgaca tgctgacgct ttactggcac tttcttggcg gactctggct
     2281 gatcctgttc ctttatttcc tctggttttt cgtgtgaccc ggggacggta aaccggggcg
     2341 ttcgcagccg gggatcccgc cctgcagcac aaaaaatcga taattcccgc ccctgtttct
     2401 gtccggaaag ctggttatat ttagctgttc aatggaaaat ccggaaaaca ggttttacct
     2461 cgaccagtgg caccaggaag tgaggcgcct catgatcaaa tcgtcgagcg ccagctggcc
     2521 gctgacgtcg ttgtgccttg ccgcgttcat ttttgtcgag atctatcacc gtaaccagcc
     2581 ggactacatc actccgttct attttctttt cctgggctgc agcgttctcg tgatgagcgc
     2641 gcacctgctc cgcaaaaaaa taaaatatcc ttacatcatc aatgcattcg gcacatccat
     2701 ccttgttccg atcgtaacca ccatcgccgc ctgcataacg gtgtcggaaa atgtgttcac
     2761 ctatttcctg atgggctccg tggtgatcgt cgtgcgggga ttgctgtatt gccagaaagt
     2821 aaaaacgctg gcgatcatag ccgtatgcag ccacgtgctg atttttacca cggtgcttct
     2881 tgtccgcaaa gaaccgtacc tctcgctgcc caatatttta agcacgaaca tcatccagct
     2941 tttcctcgtg atgttttcac tctcgggcac cgcttcgcgc tacaagctta cgcgtgataa
     3001 tttcctgaac tcgctccggc tggccgacgt caacaagacc atcgctgaaa aaaacaagga
     3061 catcaccgac agcatcaatt atgcgagacg cattcagcgc tcactgctgc cacaacagca
     3121 tttcctggat tcgaatatcg gggagcattg cgtcttttac aggccgaagg atatcgtgag
     3181 cggagatttt tactgggctt cgcgtttgca gaacggggat tttgccatcg tggtcggcga
     3241 cagcacgggc cacggtgtgc ctggtgcgat catgagcatg ctcaacatcg ccagcctgaa
     3301 tgaaattcag aaccacacgt tcatcacgtc tcctgcaagc gtgctcgatc acgcacggaa
     3361 acggatcatc gaacacctcg acaatcacga aaatgaagaa ggggcgaagg acggcatgga
     3421 cgcgatcgtg gtctgcattg acaaggaatt taaaaagctg acctacgctg ctgcgaacaa
     3481 ttccctgtgg ctcgtgagga acggggaagt agaccagctc aagggcgaca agatgtccgt
     3541 gggaaaagat gcgaacgtcc ggcatgcatt caccgagcga acgattgccc tgcaaaagaa
     3601 cgacctgctc ttcatgttca ccgacggcta taccgaccag ttcggcggcg cgcgaggaaa
     3661 aaaattaatg atcaaacgct tccgcgaaac gatgcggaac atcgcgcacc tgccgttaca
     3721 ggagatgcgg gagaaactgg cggaccattt cgatgaatgg aaaggtgagc acgaacaaac
     3781 cgatgacgtg cttgtttttg ccatgcgcgt gaactgacga agcgcttttc agcaataaaa
     3841 cggaattgaa acggactatc ctgcgacggg aacaagtccg tggtggagca tcgcctgttt
     3901 cagctttgcc atatcaggcg gaccgccggc gttgatcact tccgcgacgt ctttgaaaaa
     3961 gtccggaccg aaaacgcccg ggttggccac gcaaagaaat tcgatcgtct catccgtttt
     4021 gtttgcgaag gcatgcaccg cgcccaggga atgaagagtg aatcgcccgg atgaagctcg
     4081 atggtttgcc cgtcaaccgt ataagttgcg acgcctttca aaccataaac gggctcatcg
     4141 aaattttcgt ggtaatgcgc cgcagcgata taacctgctc ctcggtaaat ttcatcgggg
     4201 ctcatttttt tgtttcaata aaattgccgc tctacaaaaa aggattgcct acaagcccca
     4261 taaaggaatg taagacgatg aacgaaatga ctctttctca ttttccaggc cattcatatt
     4321 ctactctaat tcctgggtat atatgttgag gattggcaac attaatgtac cgttcactat
     4381 tccgaatcgt cacaatctct ctgcctgttc ctgtaatctt tagtttaaac aaatcgtcca
     4441 tgaatgcgcg gtcagtcaat cgagtatccc tttcaaaatc ggagaactta tacttcaaag
     4501 tatcatatgg agtataaact tgcagaaaaa tatcgtcttg aatattgaat tggagaattg
     4561 cgtatcctcc ttttgtgaga tagagatttt gcttcgtacc gaggcttccc aatgt
//
LOCUS       DASTSF010000590         3125 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1680885,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000590 DASTSF010000000
VERSION     DASTSF010000590.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3125
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1680885"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..246
                     /locus_tag="VFU15_16675"
     CDS             <1..246
                     /locus_tag="VFU15_16675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719481.1"
                     /translation="VCDPAGCTLAIDTTVSVDHVSATVSAGTAPYQFEWNIISGDPLV
                     SFSGDTLTVTNNGWTIEVIVTDAAGCRTSMILTQAKR"
     gene            246..1187
                     /locus_tag="VFU15_16680"
     CDS             246..1187
                     /locus_tag="VFU15_16680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719482.1"
                     /translation="MKKTQQYFLAFCLLAISSGIRAQNTSCSYPQFFCTDTSFAYSHH
                     GKGESCSPSPLYLWYYFQVPDTITSVTVSCAGGMTGCDVWGPIDSLTDACSVSSPVLF
                     SSASSTTSYTVNDGTQIPPGYYYMRLKMSSCESTVSFTVNGGYLECEQVPCDNCIGSF
                     APEPGKKYLVSVWVKEAGAAVTKTSYDHPQVYIDFPGTAVTYGPFTASGQIIDGWQRV
                     EAEFVVPNGATKIDVKLDCTSGDCYFDDLRIYPFDGSMKTYVYDPVTMRLVAELDERN
                     YATFYEYDEEGKLIRIKKETERGIMTIQESKTSLIKQ"
     gene            1195..1968
                     /locus_tag="VFU15_16685"
     CDS             1195..1968
                     /locus_tag="VFU15_16685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719483.1"
                     /translation="MNRRKNIRLGISIAGAMLLLLFADAFPVAWTDMTKEEFDGEMQK
                     TSQFYTVNQHYSVQVDYASFRGYDSEIPYDQSTGYYRKDGKSYHNFLLGIHTIQNSRY
                     KVIVDTAKKAILVADPENEESAAEMKQLNFDNESKYVTAYKSAPSGGGKLLRVEFNKI
                     PAYTAYTIDISDDGRLRGMTIYYRESYPLDPSDPSSAKAQPKLKITYSAFDAGKTFDS
                     KKEFDTSKYFTEADGKLTLTAAFKNYTITDLRIPTKKKS"
     gene            1973..>3125
                     /locus_tag="VFU15_16690"
     CDS             1973..>3125
                     /locus_tag="VFU15_16690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719484.1"
                     /translation="MKNLLTRRFMKRLLSFSFLLFTAQLVHAGSVEATRRIYSATPSD
                     TVKVGTSAAKDTVNLTPKTGSFTDFLVTDVVTFGVDHHYIKYIDTAMDVRIRLHIQPY
                     DASDSPLTAFYQNLVIKYHPSDTDTVSFVDKAVYRFTGAYKFIFTIDSVIIDNVADSI
                     LPQNMYVDGLIHAERYYDFSSNASTAIVINTLTDADLDCDDTLDQVEVSWPVMSGAEE
                     YQLEWTFVNDYDTVPSAFLAASALSFDFRNNSTRITTTNNYYDIPLLFDHGYIIFRVR
                     AIGRDEADPTQIIVGVWSDSDDGTVDDALSSYHVTTPHEASLNWQVTTTFAEDGKKKE
                     VISYFDGSLRNRQTVTRINSDDNVIVGETMYDHQGRPAINVLPAPVTYPA"
BASE COUNT          794 a          872 c          787 g          672 t
ORIGIN      
        1 gtctgcgacc cggccggctg cacgctggcg atcgatacga cggtcagcgt tgaccatgta
       61 tccgcaacgg taagcgcagg aacagcgcct taccagttcg aatggaacat catcagcggc
      121 gatccgctcg tttcattctc cggcgacacg ttaaccgtga cgaacaatgg ctggacgatc
      181 gaagtgatcg taaccgacgc ggccggatgc cggacaagca tgatccttac tcaagccaag
      241 cgataatgaa aaagacgcaa cagtatttcc tcgctttttg cctgctggcg atatcgtccg
      301 gcattcgcgc gcagaacact tcgtgcagct acccgcagtt tttctgcacg gatacctcgt
      361 ttgcctacag ccaccacggc aagggcgaat cgtgcagtcc gagcccgctc tacctctggt
      421 actatttcca ggtgccggac accatcacct cggtcaccgt ttcctgcgcc ggcggaatga
      481 cgggctgcga tgtctggggg cccatcgact cgcttacgga cgcctgttcg gtttcctcgc
      541 ctgtgctttt ctcgtcggcg tcttcaacga catcctacac ggtaaatgac ggaacccaga
      601 ttcccccggg atattattac atgagactga aaatgtcttc ctgcgaatcc accgtcagtt
      661 ttacggtcaa cggtggttac ctcgaatgcg aacaggttcc ctgcgataat tgcatcggtt
      721 cgttcgcgcc cgagccgggg aaaaaatacc tcgtcagcgt ctgggtgaaa gaagccggtg
      781 ccgcggtgac gaaaacctcc tacgatcatc cgcaggtata catcgatttt cccggcacgg
      841 ccgtgacgta tggtccgttt accgcatccg ggcagatcat cgacgggtgg cagcgcgtgg
      901 aagcggagtt cgttgttccc aatggcgcaa ccaagatcga cgtgaagctc gactgcacaa
      961 gcggcgattg ttatttcgac gacctgcgca tctacccgtt cgacggcagc atgaaaacct
     1021 atgtgtacga tcccgtgacc atgcggctcg tcgctgagct cgacgagcgg aattacgcca
     1081 cgttctatga atacgacgag gaaggaaagc tcatccgcat caaaaaagaa acggagaggg
     1141 gaatcatgac catccaggaa tccaaaacga gcctgatcaa acagtaagcg gaaaatgaac
     1201 agaagaaaaa atatccggct ggggatttcc atcgcaggtg cgatgctgct gctgcttttt
     1261 gcagatgctt ttcccgtcgc atggaccgac atgacaaagg aagaattcga cggtgaaatg
     1321 cagaagacgt ctcaattcta tacggtgaac cagcattatt ccgtgcaggt ggattacgct
     1381 tctttccgcg gttacgattc ggaaatcccg tacgaccagt ccacaggcta ttaccggaaa
     1441 gacgggaaat cctatcacaa cttcctgctc gggatacaca cgatacagaa cagcaggtac
     1501 aaagtcatcg tcgacacggc gaagaaggcc atcctggtag ccgacccgga aaacgaagaa
     1561 tccgccgcag aaatgaagca gctcaatttc gacaacgaat cgaagtatgt caccgcgtat
     1621 aaatcggctc cgtcaggcgg gggaaaatta ctccgcgtgg aattcaataa aataccggcg
     1681 tacacagctt acacgatcga tatttcagac gatggccgtc ttcgcgggat gacaatttat
     1741 taccgggaat catacccgct tgatccttcg gatccatctt ctgcaaaagc gcagcccaaa
     1801 ctgaagatca cgtattccgc cttcgatgcg ggcaaaacat tcgacagcaa aaaagaattc
     1861 gacacttcaa agtatttcac ggaagcagat ggtaagctta cgcttactgc cgcattcaaa
     1921 aattacacga tcaccgatct gagaatcccc accaagaaaa agtcctgaga tcatgaaaaa
     1981 cttactcaca cgccgtttca tgaagcgact cctgtcgttc tccttcctgc ttttcactgc
     2041 acagctcgtt catgcgggtt cggtggaagc aacaaggcgc atttacagcg ctacgccgtc
     2101 ggatacagtg aaggtcggca cttccgcggc aaaggacacc gtaaacctca caccgaaaac
     2161 cgggagcttc acggatttcc tcgtcaccga tgtggttacg ttcggtgtgg accatcacta
     2221 cataaaatac atcgacaccg ccatggatgt gcgtatccgc ctgcacattc agccgtacga
     2281 tgcgagcgac agcccgctca ccgcgtttta tcagaacctc gtcataaaat atcatccgtc
     2341 ggataccgat acggtgagct tcgtcgacaa ggctgtttac cggtttaccg gcgcgtataa
     2401 attcattttt acgatcgact cggtgatcat cgacaatgtg gccgattcta ttttgccgca
     2461 aaacatgtac gtggatggat tgatccatgc ggaacggtat tatgattttt cttccaatgc
     2521 atctacggcg atcgtcatca acacattaac ggatgcggac ctcgactgcg acgacacgct
     2581 cgaccaggtg gaagtttcgt ggccggtaat gtccggggcg gaagagtacc agctcgaatg
     2641 gaccttcgtg aacgactacg atacggtccc ttccgcgttt cttgcggcat ccgctctttc
     2701 atttgatttc cgcaacaaca gcacgcgcat cacgacgacg aacaactatt acgatatccc
     2761 gctgctgttc gatcacggct atatcatttt ccgcgtgcgt gcgatcgggc gtgacgaagc
     2821 cgacccgacg cagatcattg tcggcgtatg gagcgacagc gacgacggga cagtggatga
     2881 tgcgctatcg agctaccatg tcacaacacc gcatgaagca tcgctgaact ggcaggtgac
     2941 gaccacgttc gccgaagacg ggaagaaaaa agaagtgatc agctatttcg acggctcgct
     3001 gcgcaaccgg cagacagtga cgagaataaa ctccgatgac aacgtcatcg ttggcgaaac
     3061 catgtatgac caccagggac gacccgcgat caatgtgctt cccgctcccg tcacttaccc
     3121 ggcat
//
LOCUS       DASTSF010000591         5744 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_52448,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000591 DASTSF010000000
VERSION     DASTSF010000591.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5744
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52448"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="VFU15_16695"
     CDS             complement(<1..195)
                     /locus_tag="VFU15_16695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719485.1"
                     /translation="MKHLFYAGAFFLSALMLSCGNGGKSSEANDSLTKQAARAKYQAA
                     VDSVEKLLHSGKSYDQRLATA"
     gene            complement(216..788)
                     /gene="def"
                     /locus_tag="VFU15_16700"
     CDS             complement(216..788)
                     /gene="def"
                     /locus_tag="VFU15_16700"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007650226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEU4719486.1"
                     /translation="MILPIVAYGDPVLKKVAKEIASDHPGLDKLISDMFETMYNAKGV
                     GLAAPQIGKSIRLFIVDAKPFSEDAETEEEAAMAHFRRIFINARIHEENGDPWKFNEG
                     CLSIPKIREDVVRRPKIRISYVDETFTKQEEIFEGIAARIIQHEYDHIEGKLFTDRIS
                     PLRKRLLMGKLNDISRGNIEVDYKMRFPVK"
     gene            856..1344
                     /locus_tag="VFU15_16705"
     CDS             856..1344
                     /locus_tag="VFU15_16705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATPase"
                     /protein_id="HEU4719487.1"
                     /translation="MNSIPGMPPGARVWIYQSDRGISPAEKKQILEKAGVFLADWTSH
                     GAMMQAGIDVLHDHFLVVAADEEKAKASGCGIDKSVRFVRETGQELGIDFFDRMNVAY
                     RDNTGKIAVMKLPDFEKAVENGIVSADTIVFNNLISTAGDLERSWEVPLRDSWHARML
                     VH"
     gene            1354..1869
                     /locus_tag="VFU15_16710"
     CDS             1354..1869
                     /locus_tag="VFU15_16710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719488.1"
                     /translation="MQKYRRVLMFVFLAWGIAMLFIPVRKETISGFYKGDTPDTRIFV
                     EQHQVDYSSVALGFSGHTGGIFALAFFMLMPFFIFAEAGSFKRPFSLPARAFLQLQAL
                     LLFLGAPYCFYIATFEGGYYYDAVHSTTLAAGGWILFAQNIIFGAFLFSALGFPNGKT
                     ASLFGSRMKEA"
     gene            1897..2448
                     /locus_tag="VFU15_16715"
     CDS             1897..2448
                     /locus_tag="VFU15_16715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719489.1"
                     /translation="MNKRALFFTTAIAWSILSFFIPLVSRPVEGSPAAEISHARGYEL
                     LTGANLPWSFLVIVILAYPLIVAVPVAFLRKPFRVHSCFILAIVAVLFPLALYCAYLF
                     IELVEMWLDFLSDLLSGILESLAGPDPNTETANTAQADFTLFTPWAWSIMVTNSVLAV
                     SLLLACFRPGGRAGSFFREREAV"
     gene            complement(2463..4052)
                     /locus_tag="VFU15_16720"
     CDS             complement(2463..4052)
                     /locus_tag="VFU15_16720"
                     /inference="COORDINATES: protein motif:HMM:NF024500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OstA-like protein"
                     /protein_id="HEU4719490.1"
                     /translation="MIIATFPDAMRVNRIFLFLIVLTGFFHCEVSAQTSPTDGKQVNV
                     KHADVLKGDKSHGYQRLVGNVVFEHQGTIMTCDSAHFFLEQDSLNAFGHVHISQGDTV
                     NIWGDRLNYNGNTRQAQLLKNVRMTDKDMTLTTDAINYDMNAKVASYSSGGKIISKAN
                     TLTSQIGAYSTEAKMLTFKKNVVLLNPEYVMNCDTLRYMPVPKVAYFIGPTTIKANAN
                     KNFIYCENGFYDTFNDVCQFQKNAYILTQDQELRGDSIWYNRRTGLGKAMQNVQIIDT
                     AQKMTITGDLAIHNENTDVSTVTGHALYIQAFSKDSLFLHADTLKAVTIADTTKRNED
                     DTAFKKQAPKRNVFAYRNVRIYKPDMQGKCDSLFWASSDSIMQMFGDPVLWSGGSQLT
                     SETIFIHTSHGEISQLDMQNSAFIVSKEDSGQFNQIKGKKMTGYFKNNELNRIYVEGN
                     AQTLYYAKDDSTLIGINRADCTRILIGVEDDEVKTIMFYDSPEATLYPPGDLSPKEAL
                     LKDFHWRGDEQPKTVDDLFLR"
     gene            complement(4049..5338)
                     /locus_tag="VFU15_16725"
     CDS             complement(4049..5338)
                     /locus_tag="VFU15_16725"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006259442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEU4719491.1"
                     /translation="MKADVLLGLQWGDEGKGKIVDVLTPKYDVIARFQGGPNAGHTLE
                     FNGIKHVLHTIPSGIFHDSTINVIGNGVVIDPVILKKEIEALRKLGVDVKKRLLVSRR
                     AHLILPTHALLDKASEAAKGNAKIGSTLKGIGPTYMDKTGRNGLRVGDIDLPDFRDKY
                     NKLVEKHLELLRFLNSGENPASLEAAWFEGIETIRTLTRIDSEHYLNEALVSGKKVLA
                     EGAQGSLLDIDFGTYPFVTSSSTMCAGACTGLGLSPGKIGGVIGIFKAYCTRVGSGPF
                     PTELIDDENGPGSRMRKTGNEFGSTTGRPRRCGWLDLPALRYAIMINGVTELMMMKAD
                     VLSIFDSIRICTSYRIDGKTTDKLPYDLCGLPVEPVYVELKGWNKDLTGITSAGEMPV
                     ELQDYISFIERETGVPVSIVSVGPDRTQTLMRTEVWA"
     gene            complement(5335..5688)
                     /locus_tag="VFU15_16730"
     CDS             complement(5335..5688)
                     /locus_tag="VFU15_16730"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEU4719492.1"
                     /translation="MSFTYNIIRKDPEVVLSLYGELIDRTQSQDFVSLTESFFEEGKN
                     RFILDLSELRYMNSSGLNLLITLLTRSRKNGGDLILCGISQKVRELLLITKLNSVFTV
                     TDTVEEAAARFNTGS"
BASE COUNT         1178 a         1454 c         1573 g         1539 t
ORIGIN      
        1 ggcggtggcc agccgctggt cgtatgattt acccgagtga agcagctttt ccacgctgtc
       61 gaccgctgcc tggtattttg cgcgggccgc ctgtttcgtg aggctgtcgt tcgcttcaga
      121 ggattttccc ccgtttccgc agctgagcat tagcgctgaa agaaaaaaag cgccggcata
      181 aaaaagatgt ttcatgtgag attgggttga tatttttatt tgacggggaa tcgcatttta
      241 taatcgacct cgatgttccc gcgggaaatg tcattgagct ttcccatcag caggcgtttg
      301 cggagcgggc tgatcctgtc ggtgaacagc tttccttcga tgtgatcgta ttcgtgctgg
      361 ataatgcgtg ccgcgatgcc ctcaaatatt tcttcctgct tcgtgaatgt ttcatccacg
      421 tagctgatgc ggatcttcgg cctgcgcaca acatcctcgc ggatcttcgg aatgctcagg
      481 cagccttcgt tgaatttcca cggatcaccg ttttcctcgt ggatgcgtgc attgatgaat
      541 atcctccgga aatgcgccat cgccgcttct tcttcggttt cggcatcctc ggagaaaggt
      601 tttgcgtcga caataaaaag gcggatgctt ttcccgatct gaggggcggc cagtcctacg
      661 cctttggcat tatacatggt ttcgaacatg tcggaaatga gcttgtcgag cccgggatga
      721 tcgctggcaa tctctttcgc cacttttttc agtaccgggt ctccgtaagc tacgatcggc
      781 agaatcatgc ggcaaagata agagaagaaa aaactgtttt gcctttccgg cttcactttg
      841 cgctatcttt gaggcatgaa ttctattccc ggaatgccgc ccggtgcgcg cgtctggatc
      901 taccagtcgg atcgcggaat ttcgccggcg gaaaagaagc agatactgga aaaagccggc
      961 gtatttctcg ccgactggac ctcgcacgga gcgatgatgc aggcaggcat cgacgtattg
     1021 cacgatcatt tcctggtcgt tgcggcagat gaagaaaaag cgaaagcgag cggatgcggc
     1081 attgacaagt ccgtgcgttt cgtgcgcgaa acagggcagg agctgggcat tgattttttc
     1141 gaccgcatga acgtggctta ccgggacaac accgggaaga tcgcggtgat gaagctcccg
     1201 gattttgaaa aggcggtgga aaacgggata gtaagcgcgg atacgatcgt gttcaataac
     1261 ctcatcagca cggcaggcga tcttgaacga tcgtgggagg ttccgctgcg cgacagctgg
     1321 catgcccgca tgctggttca ttaaaatttc atcatgcaaa agtacaggcg tgtgttgatg
     1381 ttcgtttttc tcgcctgggg aatcgcgatg ctgtttattc ccgttcgcaa agaaaccatc
     1441 agcggctttt acaaaggcga tacgcccgat acccgcattt tcgttgagca gcaccaggtt
     1501 gactattctt ccgttgccct tggcttttcg ggccatacgg gcggaatttt cgcgctggct
     1561 ttcttcatgc tgatgccgtt tttcattttc gcggaagcgg gttcattcaa acgcccgttc
     1621 tcgcttcctg cacgcgcttt cctgcagctg caggcgctgc tcctgtttct cggcgcgccg
     1681 tattgttttt acatcgcgac attcgaagga ggatattatt acgacgccgt tcattccacc
     1741 acgctcgccg cgggaggctg gatattgttc gcgcagaata ttattttcgg ggcgttcctt
     1801 ttttccgccc tcggttttcc caatggaaaa acggcttccc tgttcggaag caggatgaaa
     1861 gaagcctgaa gcatgaacac ggctgtgaaa ttaccgttga ataaaagagc tcttttcttc
     1921 acaacggcta tcgcctggag cattctctct ttttttattc ccctggtctc acgacctgtt
     1981 gaaggttctc cggcagcgga aatttcccat gccaggggct atgaattgct gaccggtgcc
     2041 aacttgccct ggtcgttcct ggttattgtt atcctggctt acccgctgat tgtagctgtt
     2101 cctgtcgctt ttctccggaa gccgttccgg gttcattcct gttttatcct cgcgatcgtc
     2161 gcggtcttgt tcccgctggc gctttactgc gcttatcttt ttatcgagct ggtggaaatg
     2221 tggctggatt ttctttcgga cctcctgagc ggaatactgg aaagcctggc gggtccggac
     2281 ccgaacacgg aaacagcaaa tacggcgcaa gctgatttca cgttgttcac tccgtgggcg
     2341 tggagcatca tggtcacgaa cagcgtactt gcggttagcc tgctgctcgc gtgtttccgt
     2401 cccggcggca gggcgggttc atttttcagg gaaagggaag cggtatgaaa tacgccgcag
     2461 gatcagcgga gaaaaagatc atccaccgtt ttcggctgct catcgccgcg ccagtgaaaa
     2521 tctttcagca gtgcttcttt gggtgaaaga tctcccggcg ggtaaagcgt ggcttcgggg
     2581 ctgtcgtaga acatgatcgt tttcacttcg tcatcttcca caccaatgag gatgcgtgta
     2641 cagtcggcgc ggttgatgcc gatcagcgtg ctgtcgtctt tcgcgtaata caatgtctgc
     2701 gcatttccct cgacatagat tctgttcagt tcgttgttct tgaaataacc ggtcatcttt
     2761 ttccctttga tctggttgaa ctggccggaa tcttccttcg acacgatgaa cgcgctgttc
     2821 tgcatatcga gctgcgaaat ttccccgtgc gaggtgtgaa tgaaaatcgt ttccgaggtg
     2881 agctggcttc cgcccgacca gagcacagga tcgccgaaca tctgcatgat ggaatcggaa
     2941 gaggcccaga aaagcgaatc gcattttccc tgcatatcgg gcttgtaaat gcggacattc
     3001 cggtaagcaa aaacattgcg tttcggtgcc tgctttttga aggcggtatc gtcttcattc
     3061 cttttcgtcg tatcggcgat cgtaactgct ttcagcgtat cggcgtgaag gaaaagtgaa
     3121 tccttgctga acgcctggat gtacagcgcg tgtcctgtta ccgtcgaaac gtcggtgttc
     3181 tcgttatgga tggcaagatc gccggtgatg gtcattttct gcgccgtatc gatgatctgc
     3241 acgttctgca tcgctttgcc gaggccggtc ctgcggttgt accagatgct gtcgccgcgc
     3301 agctcctggt cctgcgtaag tatgtaggcg tttttctgga actggcagac atcattgaac
     3361 gtgtcgtaaa aaccgttctc acagtaaatg aaattcttgt ttgcgtttgc tttgatggtg
     3421 gtaggaccga tgaagtacgc cactttcggg acgggcatgt agcgaagcgt gtcgcagttc
     3481 atcacgtatt cgggattgag aagcaccacg ttttttttga acgtcagcat ttttgcttcg
     3541 gtggaatacg cgccgatctg cgaagtgagc gtgttcgcct tgctgatgat ctttccgccg
     3601 gatgaatagc tcgccacttt tgcgttcatg tcgtaattga tcgcatccgt tgtcagcgtc
     3661 atgtctttgt ccgtcatgcg gacgtttttc agcagctgcg cctgccgcgt gttgccgttg
     3721 tagttgagcc ggtcgcccca gatgtttaca gtatcgccct gcgaaatatg cacgtggccg
     3781 aacgcattca gcgaatcctg ctcgaggaaa aaatgtgcgc tgtcgcatgt catgatggtt
     3841 ccctggtgct cgaaaacgac attgccgacg aggcgctggt aaccgtgcga tttatcacct
     3901 tttaacacgt cagcgtgctt tacattgacc tgtttgccgt cggttggaga tgtctgcgcg
     3961 gaaacttcac agtgaaaaaa tcccgtgagc acaatgagaa aaagaaaaat ccggtttacg
     4021 cgcatggcgt cgggaaatgt ggcaataatc atgcccacac ttcagtgcgc atgagcgtct
     4081 gcgtgcggtc aggtccgaca gaaacgatag aaaccggaac gccggtctca cgttcgatga
     4141 aggaaatata atcctgcagc tcgacgggca tttcgcctgc cgaagtgatg ccggtgagat
     4201 ctttgttcca tcctttcagc tcgacgtaaa cgggctcaac gggcagtccg cagagatcgt
     4261 aaggaagctt gtcggttgtt ttcccgtcga tcctgtacga cgtacagatt cttattgaat
     4321 caaaaatgct gagcacatcg gctttcatca tcatcagctc tgtcacgccg ttgatcatga
     4381 tcgcatagcg cagcgcggga aggtcgagcc atccgcatcg tctcgggcgg ccggtggtgg
     4441 agccgaactc atttcccgtt ttccgcatgc gggagcccgg accgttttca tcgtcgatga
     4501 gctcggtggg gaagggaccg cttccgacgc gcgtgcagta cgctttgaaa attccgatca
     4561 cgccgccgat ctttccgggg gaaagaccga gtcccgtaca cgcgccggcg cacatggtgc
     4621 tggacgacgt cacgaaagga tacgttccga aatcaatatc gagcagtgag ccctgcgcgc
     4681 cttcggcaag cacttttttt cctgaaacaa gcgcttcatt gaggtaatgc tcgctgtcga
     4741 tccttgtcag cgtgcggatc gtttcaattc cttcgaacca cgcagcttca agcgaagccg
     4801 ggttttcacc ggaattaaga aaacggagca gctcgaggtg cttttcaacg agcttgttgt
     4861 atttgtcgcg aaaatcgggg agatcgatgt ctcccacgcg cagcccgttg cgtcctgttt
     4921 tgtccatgta cgtcggcccg atgcccttga gggtggatcc gatcttcgcg ttgcccttcg
     4981 ctgcttctga cgctttatcg agcaatgcgt gcgtaggaag aataaggtgc gcccttcttg
     5041 aaacgagcag gcgttttttt acatcgacgc cgagcttgcg cagcgcttcg atctcttttt
     5101 tcaggatgac agggtcgatg acgacgccgt tcccgatcac gttgatcgtg ctgtcgtgga
     5161 aaatcccgga aggaatggta tgaagaacgt gcttgatgcc gttaaactcg agcgtatgtc
     5221 ctgcgttagg tccgccctgg aaacgcgcga tcacatcata ttttggagta aggacgtcaa
     5281 cgatctttcc ttttccttca tctccccatt ggagtccgag taaaacatct gccttcatga
     5341 tcctgtatta aaacgggcag ccgcttcttc gacggtgtcc gtaacggtaa aaactgaatt
     5401 gagcttggtg atgagcagga gctcgcgcac tttctgcgag atgccgcaga gaatgagatc
     5461 gcccccgttc ttgcgtgagc gggtgagcag cgtgatcagc aggttgagac ccgagctgtt
     5521 catgtaccgg agctcggaaa gatcgagtat gaagcggttc tttccttctt caaagaaaga
     5581 ttccgtcagc gaaacgaaat cctgtgactg cgtccggtcg atcagctctc cgtatagcga
     5641 gagaaccact tcggggtctt tccggatgat gttgtaagtg aaactcatga actttttttt
     5701 gcgggtgggg taaggttatc gcaggtgccg tttttggcga gctt
//
LOCUS       DASTSF010000592        13780 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_342068,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000592 DASTSF010000000
VERSION     DASTSF010000592.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 13780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13780
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_342068"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(56..934)
                     /locus_tag="VFU15_16735"
     CDS             complement(56..934)
                     /locus_tag="VFU15_16735"
                     /inference="COORDINATES: protein motif:HMM:NF025224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HEU4719493.1"
                     /translation="MKKYYAAPVAFLSVVSVHAQQPPSNWDVSAPQGSMVYTDLKTAE
                     KEAANVYRLDLSGAGDFRKDKKNMERVASLSNLMALRLGNNSMTQLPPVFLQLPSLVY
                     FGSSGNAFTTFPDSIGMWSNLKYMELGNTAFDSLPVAIYGLPKLQELSVNRNADTLLI
                     PYGISSMAYSLQEFKIYNTIIDSLPADFSSLDKLKKLVLYKAGFKIFPREILGMHGLN
                     ELYLDSNNISVLPRAIGEMKGLTYLSLRGNRLTHIPSTICFLQNLSVLDLRGNPLDPY
                     EVTCLQALLPSCEILF"
     gene            complement(1173..4157)
                     /locus_tag="VFU15_16740"
     CDS             complement(1173..4157)
                     /locus_tag="VFU15_16740"
                     /inference="COORDINATES: protein motif:HMM:NF012907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HEU4719494.1"
                     /translation="MKKLILLPALLFSCTAFSQNSSVVQEQSAHYGAMGPHDDAYWDQ
                     VRQSEGEIISSAPQVQNVSTCTLNKRVFGWHPYWNGSTYTNYQWNLLSDFCYFDFTVS
                     PNTGHNTNASYAWSTSAAVTAAISNGVNAHICATLFSSHSTFLGSATAMQNFISDIIS
                     DLQARGGKGVNIDFEGMTASNSAPFTAFIQNLSTQLHAAIPGSEVSIALYAVDWSAVF
                     DIPNLSPYVDLFIIMGYDYYWSGSTTAGPEDPLYDFETSYNYTLTRSITYYLKQGMPA
                     SKLLLGLPYYGREWETTGSSAPSATTGAYTATKTFSTVMANTNGYYSSPQWEPNSFSV
                     YYPYQVSANWRQCFCDNDFSMRKRFDIVNQRNIGGIGIWALGYDDGYMDFWNAIQDKF
                     TNCATVPCSDTLYDMGGPNRNYYDAENYTTTIAPTGASAVSLSFSQFDVELNYDTLWI
                     YNGATTASPLIGAYTGTNSPGTVTSTGPALTIRFKSDNATTRPGFTAVWQCMMDNTPP
                     ATQVTTTGNWQTQNFPATFADTDNQGGSGIEKSFYQVCDYNGNDWRANNADGFFNDDF
                     SQPVISSDWTTMVGTWAVNSSGQLEQSDESQSNTNIYAPLTQNLSNRYVYCWDGAISG
                     TGNNRRAGFHFFCDDATQTNRGNSYFVWFRVDQGTLEFYKVVNNVFTLEQSYPMTVNA
                     GQWYNWKVIYDRITGVIEVYQDDVFAGSWTDPAPYSSGNAVSFRSGNCNWKVDNFHVF
                     RSRYSNAQTTVSVGTCPACDIRYENTSPVAPSGKIRSLTKDNAHNLSAIDETNVNVDW
                     TAPLMPAVIFDGNAADIDTTFNLSLIEGNWNAAADTNSGVYEYWYAIGTTSGDSDFVS
                     WTSNGLSQSVSLPATLTAGQWYYFSVRAENNAGLVSGNVQSDGQVAELSTGTGADETG
                     NGLNIFPNPSSGMIYISVAAAGSCTVVITDEAGREVGRFRKDASAGASVLPFDASALN
                     SGIYFVHLYTDSGTSTSRFIRR"
     gene            complement(4199..5581)
                     /locus_tag="VFU15_16745"
     CDS             complement(4199..5581)
                     /locus_tag="VFU15_16745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719495.1"
                     /translation="MKKIIAGLIVLAVVQLHAQVRNPRYDTAGIYPAGNIHAADPRGD
                     SLAAFYSHKAAALMDRDTALGDFYRIDNTGVYMFAGAGMKAQNVPEYFIAWNELDIFR
                     NIVSQNSHDDAMCILLAKGDRPFSKDVAYKYGKKKSPPLHFFMQEKPLNGFRFAIDPG
                     HFASDSATAHIEDKYLDFYENVPGADSVRIFFWESMLTWQTAVLLAQKLRDAGAEVMM
                     TRQSYGLTAMGKTFQQWKQDDYPRCLDSLLALDPTNKNLRALKSGKKKDDRSIFRFVF
                     RDVELKKRADLINAFHPDLTVVIHYNVDNENKDWKKPTDKDFCMTFVGGSFERGELDD
                     PGKRFDFLRLLLTDDIPQSISACGIAAKQFSEDLDVPLAKSSDASYLQKDCIYAGEPG
                     VFCRNLALTRMITGPVIYGETLYEDNVKEAFLLNQTPSPAIRNDLEETRVEEVADAYF
                     RAVCNWAKLH"
     gene            complement(5578..6021)
                     /locus_tag="VFU15_16750"
     CDS             complement(5578..6021)
                     /locus_tag="VFU15_16750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719496.1"
                     /translation="MNYLLRIMIMLFFLFSAGGVHAQTKSKRTIVRENYSNGKPSKVM
                     VVRVTRSKFEDPSNYYKRTKVTTTYFDSISGHVTKKTVLITKLGESGPPCYEIFRKET
                     LYDARGKKVRMEKQRCDRRRYVLKEYRNGKLVHVLIQRRRRVQVA"
     gene            6134..6544
                     /gene="arfB"
                     /locus_tag="VFU15_16755"
     CDS             6134..6544
                     /gene="arfB"
                     /locus_tag="VFU15_16755"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013305927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HEU4719497.1"
                     /translation="MDLSALSIEKEFAIKATRAGGKGGQHVNKVSSRIELTFDIPASQ
                     VLSEEQKLILLEKLETRLSSEGVLRVTESGDRSQHENREKAIAKTIALLENALKPVKK
                     RKKTKIPKAVKAKIREKKKKLSQKKQRRRENFWD"
     gene            6609..7424
                     /locus_tag="VFU15_16760"
     CDS             6609..7424
                     /locus_tag="VFU15_16760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719498.1"
                     /translation="MKKLLFSLSLLSFLTVSATDNPPVGARAQGMAGCGTALYGDLWG
                     TQNNQAGLAFIKNFQAGAFYESRFLVSDLGMKAFGAAMPTKAGTFGLNVTSLGLGNLY
                     SENKAGLGFAKSFGPKFAASAQIDYLYTHLAENYGNASTACGEIGLLAQPYRNLWLGF
                     HIFNITRSKLSGNVDEHIPTIMRLGALYGFSDRVFVTVEAEKDIDFKPVIRGGFEYRP
                     VNNFYIRAGAGTQPGLMAFGCGIVMKKVRLDLASTFHSVLGFSPSVSLQYGIE"
     gene            7493..9532
                     /locus_tag="VFU15_16765"
     CDS             7493..9532
                     /locus_tag="VFU15_16765"
                     /inference="COORDINATES: protein motif:HMM:NF024245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEU4719499.1"
                     /translation="MKDTVPQDDPLQQQIEDLSENLSNENADYTTLTDNLRYYQQHPL
                     NLNTASREELEDLMLLDEVQIDNLLAHRDKFGPLMTIYELQSIDGFDLATIYKILPYV
                     KVSDNMDSGHFSFHEMRQNGHHEIVLRDQRVIETQKGFSPIDSAALAASPNSRYLGSP
                     DHLYARYRFTYGNYVSVGFTADKDPGEQFFRGTQKQGFDFYSGHVCVRNISFVKTAVI
                     GDYQVGFGQGLTAWTGYAFGKTSMVINTKRNATGIRPYTSVEENKFLRGAAATVRFGH
                     FETSAFFSYNHRDANIALSDTTGGKNQEVLEVSSLQVTGLHTTPSEVADKDAITEMIF
                     GGNVSYNRKRFHLGVTGMHSEYSANLVRGLSLYNQFEFSAQHNTIAGADYDWSFHNFH
                     FFGEGAMSANGGYAFVNGVLISLDPRLIFTVHQRWFDKNFQNLYANVFSESTTIANEK
                     GIYFGMMAKPLPRFTISTYVDHFIFPWLKYQVNAPSSGNDFLVQVNFTPDKKTDMYFR
                     YRHRDKFTNAGDSESPIDYIIPYAQDNYRFNIQYPAGASLRLRNRVEYSQYYKSNGKP
                     EKGFVIYQDVTYHRIGSRLSFSARYALFQTDSYNAAIYTYENDMPYTFSIPAYYYKGS
                     RVYLLVNWDISRNVELYFRISQTMYYDRNVISPGSLTEIDGNTKTEAKMMLRIKF"
     gene            complement(9556..11265)
                     /locus_tag="VFU15_16770"
     CDS             complement(9556..11265)
                     /locus_tag="VFU15_16770"
                     /inference="COORDINATES: protein motif:HMM:NF017889.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007160 - cell-matrix adhesion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nidogen-like domain-containing protein"
                     /protein_id="HEU4719500.1"
                     /translation="MNALRRIILPVIPVFLFTFLHAQSPVRIQGSGNPEYEKLKESGQ
                     LPKAPLLIVNGDTSSMPVQPRIEPADPQSSVLCNCMIPIDASFQVVPFQFATGPDYRN
                     DDGSSAQITLPFNFCFYGQSMNSCFINNNGNISFLTSYGTFTANSFPDPGFVMIAPFW
                     GDVDTRNAQSGLVYYKVTPTYMVVRWKTVGYFNAYADKVNDFQLIITDGSDPILPSGN
                     NVNFCYGDMQWTTGDASGGSGGFGGSPATVGVNRGNGTDYIQIGQFDSPGTNYDGPFG
                     NPDQVSWLDNQTFYFNVCNNGSGNNLPPIINSADVCDTIVLCIGDTTSISATFLSPEN
                     GQNTTATITSSVQGLTVTSNTSGNPAVITALLIANAANAGYNTITITGTDNGSPPAAT
                     SANVIVDIISTPAVSFTMNPPPVQPMGVNVQFTDNTPGATSWLWDFGDGQTSVLQNPT
                     HTYAHDSLYTVTLTVTVGSGCTASLTQLYLVQTDIPVIAPNVVTPNGDGKNDALVFTN
                     LQRHPDSDLIVYNRWGNIIYQHANYQNDWIPAVSDGVYYYVLSGPNLDNTITGFFEVI
                     KGK"
     gene            complement(11331..13595)
                     /locus_tag="VFU15_16775"
     CDS             complement(11331..13595)
                     /locus_tag="VFU15_16775"
                     /inference="COORDINATES: protein motif:HMM:NF017889.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HEU4719501.1"
                     /translation="MKRIAVFIFLLSVRVYSFAQNDTAVNAPMEITPQQWDQLKAEGK
                     LNTGLYVMKQGQDPQPLKTIVQPPSTNPQTVPANCNCMIPIDATFQVVPFQFGTAPDY
                     RNDDGSSPVINLPFVFCFYGNVQSQVYINNNGNISFGSPYGTFSASAFPSSFYTMIAP
                     FWADVDTRNLTSGIVYYKVTPTALIVHWQNVGYYSQHVDKLNDFQLIITDGTDPLLPP
                     GSNCGFCYGDMQWTTGDASGGSNGFGGTAATVGANMGDGVNYIQIGTFDQAGTTYNGP
                     FGPPSQISWLDNKQFFLDVCSTGGGGNLPPIMNAALVCDTLTLCVGDTLPITAQFLSP
                     ENNQTTTITASTPGTGLTTLVSTPGNPASFNAIFVGMTSNIGLNTVTLSGTDNGTPPQ
                     TTNGNVLINVIAGPTASFTSAGVCPGLPMQFNDASTTVNGPITSYHWDFGLPAQTNDT
                     SDIQSPQWVYNTPGAYPVTLTVIDSLGCKDTAIVNAQVFYLPQVQFSGGPLIGCAPLC
                     VDLADQSTVQNSVPAHWNWQYPDGGSDTIPDPNHCFANQGDYSVVLTVTSQQGCSYTD
                     SIHNYIHVIPGPQAAFSLTPQPATMSDPTIYFTDQSTAGPQEWYWDFGVSGANSTSQN
                     PNYVYPDTGSYNVMLVVSAAGGACPDTAWATLTISPELLIWIPNAFTPNGNDRNDVFT
                     PVFSDVTYVNQYDFMVFDRWGNLVFHSQDPYKGWDGRLNDQYVMSDTYVYKIFVGGID
                     GTTHSYIGSVSVIR"
BASE COUNT         3233 a         3716 c         3660 g         3171 t
ORIGIN      
        1 cgcccagcgg ttttcgcttt tcagtttatc tccctggaat gaatggttga acttttcaga
       61 ataatatctc gcacgaaggc agcaacgcct ggagacacgt cacttcgtac ggatcgagcg
      121 ggtttccgcg cagatcaagc acggaaagat tctgaaggaa acagatggtg gaaggaatgt
      181 gcgtcagccg gtttccccgc agcgacaaat acgtcagccc tttcatttcc ccgatagcgc
      241 ggggcaggac ggaaatatta ttgctgtcga gataaagttc gttcagtccg tgcatgccga
      301 gaatttcccg ggggaaaatt ttgaatcctg ctttgtacag caccagcttc ttcaatttgt
      361 ccagcgatga aaaatcggcg ggcagcgaat cgatgatcgt gttgtagatc ttgaactcct
      421 gcagtgaata cgccatcgaa gaaatcccgt acggaataag aagcgtatcg gcattccggt
      481 taacggacag ctcctgcagc ttcggaagtc cgtagatcgc caccggcaat gaatcgaaag
      541 ccgtattgcc gagctccatg tatttcaggt tgctccacat gccgatagaa tcggggaaag
      601 tggtgaatgc attcccggaa gaaccgaaat aaacgagcga aggcagctgg aggaaaacgg
      661 gcggcagctg cgtcatggaa ttattaccga ggcgcagcgc catgagattg gaaagcgagg
      721 cgacccgctc catgtttttt ttgtccttcc ggaaatcacc tgcgcccgac agatcaaggc
      781 ggtaaacatt tgcggcttct ttttccgccg ttttcaggtc cgtgtagacc atgctgcctt
      841 gcggtgcgga aacatcccag ttcgaaggcg gctgctgtgc atggacagaa acgaccgaaa
      901 gaaaagcaac gggagcagcg tagtattttt tcatttcagc ggttgtccgg aaaaagtttg
      961 agtgcgttga aaaattcttc aggcaaagga aacaacgctt ttgcgcaagt gacagccggg
     1021 aaaaaatgta tcgcaccaaa aaaatcattc cggatgtttt tcacgactgt tttcaactgt
     1081 ttaacaacga aggacactat gttttgcaga aacgctgcaa agaaaatttt ctttctcaaa
     1141 aaaatcctcc tgcctgattt gcatccggca ttttaccggc ggatgaaacg cgacgtgctg
     1201 gtcccgctgt ccgtgtaaag atgaacaaaa taaatgccgg aattcagcgc agacgcgtca
     1261 aaaggcagaa cagaagctcc cgcagaagca tctttccgga aacggcccac ttccctgccc
     1321 gcttcatccg taataacaac cgtgcagctt cctgccgcag caacggaaat gtagatcatg
     1381 ccggaagaag gattcggaaa aatattcagc ccattccctg tttcatccgc gccggtgccg
     1441 gtagacaatt ccgccacctg cccgtcgctc tgcacattgc cggaaacgag tcccgcgttg
     1501 ttctccgcac gcacggaaaa ataataccac tgtcctgcag tcagcgttgc gggaagggaa
     1561 acggactgcg aaagaccgtt cgacgtccac gaaacaaaat cggaatcgcc ggaggttgtt
     1621 ccgatggcgt accagtattc gtatacgccg gaattcgtgt cggcagctgc attccagttt
     1681 ccttcgatca gcgaaagatt gaacgtcgta tcgatgtccg ctgcattgcc gtcgaagatc
     1741 accgccggca taagcggggc ggtccagtcg acattcacgt ttgtttcatc gatcgcggaa
     1801 aggttgtgcg cattgtcttt cgtcagtgaa cgtattttcc cggaaggcgc gaccggggat
     1861 gtgttctcat aacggatgtc acaggccggg cacgttccga cggaaaccgt ggtctgcgca
     1921 ttggaataac gtgaacggaa cacatggaaa ttatccacct tccagttgca gttgccgctg
     1981 cggaatgaca ccgcgttccc gctggaatac ggcgcagggt ccgtccagct tccggcgaaa
     2041 acatcatcct ggtaaacttc gatcacgccc gtgatgcggt cgtagatcac tttccagttg
     2101 taccactggc cggcgttcac cgtcatggga tacgactgtt cgagcgtgaa aacgttgttc
     2161 acgactttat aaaattccag cgtgccctgg tcgacgcgga accagacgaa atatgaattg
     2221 ccgcggtttg tctgcgtggc atcatcgcag aagaagtgaa atccagcacg gcggttgttt
     2281 cccgttcccg agatcgcgcc gtcccagcaa tacacgtagc ggttggaaag gttttgcgtg
     2341 agcggtgcgt aaatattcgt gttcgactgc gattcgtcgc tttgctcgag ctgcccgctg
     2401 ctgttcacgg cccatgtccc caccatcgtc gtccagtcgc tgctgatgac gggttgcgaa
     2461 aaatcatcgt tgaaaaatcc atccgcatta ttcgcacgcc agtcgtttcc attgtaatcg
     2521 cacacctggt aaaagctttt ttcgatcccg cttcctccct gattatccgt atccgcgaac
     2581 gtggccggaa aattctgcgt ctgccagttg ccggttgttg tcacctgcgt cgcgggcggc
     2641 gtattatcca tcatgcattg ccacacggcg gtgaaaccgg gtcgcgtcgt cgcgttgtcg
     2701 gatttgaaac gtatcgtcag cgcaggacct gtggacgtaa ccgtgccggg cgaattagtt
     2761 cctgtgtatg cgccgatgag cggtgaggcg gttgtcgcgc cgttataaat ccaaagcgtg
     2821 tcgtagttca gctcgacatc gaactggctg aacgaaagcg aaacggccga cgctcccgta
     2881 ggcgcgatcg tcgtcgtgta attttctgcg tcgtaataat tgcggttggg tccgcccatg
     2941 tcgtacagcg tgtcgctgca gggaaccgtt gcgcagttcg tgaatttatc ctgtatcgca
     3001 ttccagaaat ccatgtagcc gtcgtcgtag ccaagcgccc agatgccgat accgccgatg
     3061 ttgcgctggt tgacgatatc aaaccgtttg cgcatgctga agtcgttgtc gcagaagcac
     3121 tggcgccagt tagccgaaac ctggtacggg taataaacgg aaaagctgtt cggttcccat
     3181 tgcggactgg aataatagcc gttcgtattc gccatgaccg ttgaaaacgt tttcgtcgcc
     3241 gtgtatgcgc cggttgtggc ggaaggcgcg gaagaacctg tcgtttccca ttcgcgtccg
     3301 tagtacggaa gaccgagcag cagcttcgat gcgggcattc cctgcttcag gtaatacgtg
     3361 atcgatctcg tcagcgtgta attataagat gtttcaaaat cgtacagcgg gtcttccgga
     3421 cctgccgtcg tgcttccgct ccagtaataa tcatagccca tgatgatgaa caggtcgacg
     3481 tacggcgaaa gattgggaat atcgaaaaca gcgctccagt cgacagcata caaggcaatg
     3541 cttacttccg atcccggtat cgcagcgtga agctgtgtgc tgagattctg gatgaacgca
     3601 gtgaaaggcg cactgttcga cgccgtcatt ccttcgaaat cgatgttcac gccttttccc
     3661 ccgcgcgcct gcaggtcgga aataatgtcg ctgatgaaat tctgcatcgc tgttgcgctt
     3721 ccgaggaacg tcgaatggct gctgaacaaa gtggcgcaga tgtgcgcgtt gacgccgttg
     3781 ctgatcgccg ccgtcactgc cgcgctcgtg ctccaggcgt agctggcgtt cgtgttgtga
     3841 ccggtgttcg ggctcaccgt gaaatcgaaa tagcagaaat cagaaagaag gttccactgg
     3901 taattcgtgt acgtggaacc gttccagtac ggatgccatc cgaaaacgcg cttgttgagc
     3961 gtgcacgtgc tcacgttctg cacctgcgga gcggaagaaa tgatctctcc ttccgactgg
     4021 cgcacctggt cccaatatgc atcgtcgtgc ggacccattg ccccgtaatg cgcggattgt
     4081 tcctgaacga cagaactatt ctgtgaaaaa gctgtgcagg aaaacaggag cgcaggcagc
     4141 aggatcagtt ttttcatggc ggaaaaagga aatgcaagtt acgccattcc ggatgaaatc
     4201 agtgcaattt tgcccagttg cagacggccc ggaaatacgc atccgccact tcttccacgc
     4261 gcgtttcctc taaatcgttc ctgatcgccg gcgagggcgt ctggttgaga agaaatgctt
     4321 ccttcacgtt gtcttcatac agcgtttcac cataaatgac cgggccggtg atcatgcgcg
     4381 tgagggcgag gttgcggcag aaaacacccg gctccccggc ataaatgcaa tctttctgca
     4441 ggtaggaagc atcgctgctt ttcgcgagcg ggacatcgag atcttccgag aattgcttcg
     4501 cggcaattcc gcaggcggaa atgctttgcg ggatgtcgtc ggtcagcagc aggcggagaa
     4561 agtcgaaccg cttccccgga tcatcgagct cgccgcgttc gaacgatccg ccgacgaacg
     4621 tcatgcagaa atctttgtcc gtcggttttt tccagtcctt gttttcgttg tcgacgttgt
     4681 aatggatgac gacggtaagg tcgggatgaa acgcgttgat gagatccgct cttttcttca
     4741 gctcgacatc cctgaacacg aagcggaaaa tggaacggtc gtcttttttc ttcccgcttt
     4801 tcagcgcacg gagatttttg ttcgtgggat ccagcgccag cagcgaatcg agacagcgcg
     4861 ggtaatcgtc ctgcttccat tgctggaatg tttttcccat cgcggtaagt ccgtacgact
     4921 ggcgcgtcat cattacctcc gccccggcat cgcgcagttt ctgggcgaga agaacagccg
     4981 tctgccacgt gagcatcgat tcccagaaaa aaatgcgcac gctgtcggcg ccgggaacgt
     5041 tctcgtaaaa atccaggtac ttgtcctcga tgtgcgccgt cgcggaatcg gaagcgaaat
     5101 gcccggggtc gatcgcaaaa cggaacccgt tcagcggttt ttcctgcatg aaaaaatgaa
     5161 gcgggggcga tttcttcttc ccgtatttat aggcaacatc cttcgaaaac gggcggtctc
     5221 cctttgcgag caggatgcac atcgcatcgt catgcgaatt ctgcgaaacg atgttccgga
     5281 aaatatccag ctcattccac gcgatgaaat attcaggcac gttctgcgct ttcatgcctg
     5341 cgcctgcaaa catatacacg cccgtattat cgatgcggta aaaatcgccg agcgccgtat
     5401 cgcggtccat gagcgcagcc gctttgtgcg aataaaacgc cgccagcgaa tcgccgcggg
     5461 gatcagccgc atgaatgttc ccggccgggt aaatgcctgc cgtatcgtaa cgcgggttcc
     5521 tcacctgcgc atgcagctgc acaacggcaa gcacaattaa accggcgatg atctttttca
     5581 tgcgacctgc actcttcttc tgcgttgtat caaaacatgc accagctttc cgttgcggta
     5641 ttccttcagc acatacctgc gccggtcgca acgctgcttt tccatgcgga ccttttttcc
     5701 gcgcgcatcg taaagcgttt ccttccggaa tatctcgtaa cacggcggac cgctttcgcc
     5761 gagcttggta atgagcaccg ttttcttcgt tacatgcccg ctgatggaat cgaaatacgt
     5821 ggtggttacc ttggtccgtt tgtaataatt ggacgggtct tcgaacttgc tgcgcgtgac
     5881 gcgcaccacc atcactttgc tgggtttccc gttgctgtaa ttttcacgta cgatcgtgcg
     5941 cttcgatttt gtctgtgcat gcacgccgcc tgcggaaaac aggaaaaaca gcatgatcat
     6001 gatccgcagc aggtagttca taagcgaaac gggaacgggg ttccggcaat gaagttacgc
     6061 aggaagtgga tttcaaaaaa atacacggcc cgtgcagcat gcaggttcgt tacttcggaa
     6121 ttacctttgc ctcatggatc tttcagcgct ttccatcgaa aaagaattcg cgatcaaagc
     6181 cacacgcgcc ggcggaaaag gcgggcagca cgtgaacaaa gtttcctcgc ggatcgagct
     6241 gacattcgac attcccgcct cgcaggttct ttccgaagag caaaagctca tccttcttga
     6301 aaagctggaa acgcgtttat catcggaagg tgtgctccgc gttacggaat ccggcgaccg
     6361 ttcgcagcat gagaacaggg agaaggcgat cgcgaaaacc attgcgctcc tggagaatgc
     6421 gctgaagccc gtcaaaaaga gaaaaaaaac gaaaataccg aaggcggtga aagcgaaaat
     6481 acgcgagaag aaaaaaaagc tctcgcagaa aaagcagcgc cgccgggaaa atttctggga
     6541 ctgattttac gccgaaatta tccacgctcc ttgcaaatcc gctccttccc gcctacttta
     6601 gagcctgcat gaaaaagctg cttttttccc tttcgcttct ttctttcctg accgtttccg
     6661 ccactgacaa tccgcccgta ggcgcccgtg cgcagggcat ggccggttgc ggaacagcgc
     6721 tctacggtga tctctgggga acgcagaaca accaggcggg ccttgccttc atcaagaatt
     6781 tccaggcagg cgctttttac gaaagccgtt tcctcgtcag cgatctcggc atgaaagcat
     6841 tcggcgccgc catgccgacc aaagcaggaa cgttcggcct caacgtcact tcgctcggcc
     6901 tcgggaatct ctacagcgaa aacaaagccg ggcttggttt tgcgaaatcg ttcggaccga
     6961 aattcgccgc ttccgcgcag atcgattacc tgtacacgca cctcgccgaa aattacggca
     7021 acgcatctac cgcctgcggc gagatcgggt tgctggcgca gccctatcgc aatctctggc
     7081 ttggctttca cattttcaac atcacgcgca gcaagctcag cggtaacgtg gacgaacaca
     7141 ttcccacgat catgcgcctc ggtgcgctgt acggtttttc cgatcgtgtt ttcgtgaccg
     7201 tggaagcaga aaaagacatc gatttcaaac ccgtcatccg cggcggattc gaataccgtc
     7261 ccgtgaataa tttttacatc agggccgggg ccggaacaca gcccggtctt atggctttcg
     7321 ggtgcgggat cgtcatgaaa aaagtccgcc tcgaccttgc ttccacgttc cacagcgtgc
     7381 tcggattttc tccttcggtc agcctgcaat acggaatcga atgaagcgca gcgccctttt
     7441 tgctttctgc tttttatttt cctgtttctg cggaaatcgt ctccgggcac aagtgaaaga
     7501 caccgttccg caggatgatc cgctgcagca gcagatcgaa gatctttccg aaaacctttc
     7561 caacgaaaac gccgactaca ccacgctcac ggacaacctc cgttattacc agcagcatcc
     7621 gctcaacctg aacacagcct cgcgcgaaga gctggaagac ctcatgctgc tcgatgaagt
     7681 gcagatcgac aacctgcttg cacaccgcga caagttcggc ccgctgatga cgatatacga
     7741 gctgcaatcg atcgacggat tcgacctggc caccatttac aagatccttc cgtacgtgaa
     7801 ggtttccgac aatatggatt ccgggcattt cagctttcac gagatgcgcc agaatggcca
     7861 ccacgaaatt gtcctccgcg accagcgggt gatcgaaaca cagaaaggat tttcacccat
     7921 tgacagcgca gcgctggcgg ccagcccgaa ttcacgctac ctcggttcac ccgatcacct
     7981 gtatgcgcgt taccgcttta cgtacggaaa ttacgtgagc gtaggattta cggcagacaa
     8041 agatcccggc gagcagtttt tccgcggcac gcagaagcag ggatttgatt tttattcagg
     8101 ccatgtgtgc gtgcggaaca tcagcttcgt aaaaacggct gtcatcggcg attaccaggt
     8161 cggcttcggg cagggactta cagcgtggac gggttacgca ttcggaaaaa cctcgatggt
     8221 gatcaataca aaacgcaacg ccaccgggat acggccgtat acttccgtgg aagaaaacaa
     8281 attcctgcgc ggggcggcgg caaccgttcg tttcgggcat ttcgaaacct ccgctttttt
     8341 ttcctacaac caccgcgatg cgaacatcgc actgtcggat acaaccggcg gaaaaaacca
     8401 ggaagtgctt gaagtgagca gcctgcaggt gacaggactt cacaccacgc cttcggaagt
     8461 ggcggacaag gatgcgatca cggaaatgat cttcggcggg aatgtttcct acaaccgcaa
     8521 acgttttcat ctcggcgtga ccggcatgca cagcgaatac agcgccaacc tcgtccgcgg
     8581 cctcagcctg tacaaccagt tcgaattttc cgcgcagcac aacacgatcg ctggcgccga
     8641 ttatgactgg agctttcaca acttccattt tttcggtgaa ggtgcgatga gcgcgaatgg
     8701 tggttatgct tttgtcaacg gtgtgctgat ctcgcttgat ccgcgcctga tattcaccgt
     8761 tcaccagcgc tggttcgaca agaatttcca gaatctttac gccaacgttt tttcagaaag
     8821 cacgaccatc gccaatgaaa aagggatcta tttcggcatg atggcgaaac cgcttccccg
     8881 ttttacgatc agcacatacg tggatcattt catttttccc tggttgaaat accaggtgaa
     8941 cgcgccgtcg tccgggaatg attttctcgt gcaggtcaat tttacgccgg ataaaaaaac
     9001 cgatatgtat ttccgctacc gtcaccgcga caaattcacc aacgcgggcg acagcgaatc
     9061 cccgatcgat tatatcattc cgtatgcgca ggacaactac cgcttcaaca tccagtaccc
     9121 ggccggcgct tcgctccgcc tccgcaaccg cgtggaatat tcacagtatt acaaatcgaa
     9181 cgggaagccg gaaaaaggat tcgtcattta ccaggatgtc acgtaccaca ggatcggaag
     9241 ccggctttcg ttttccgcgc gctatgcgtt attccagacc gacagctaca atgcggcgat
     9301 ctatacttat gaaaacgaca tgccgtatac gttttccata ccggcgtatt attacaaagg
     9361 atcacgtgtg tacctgctcg tgaactggga catctcccgc aacgtggaat tgtatttccg
     9421 catttcacag acgatgtatt acgaccgcaa cgtgatcagt ccgggttcgc tgacggagat
     9481 cgacggcaat accaaaacag aagcgaagat gatgctgcgg ataaaattct gatttttcct
     9541 tcgcctgccg gaagctcact tgccttttat cacctcgaaa aatcccgtga tcgtattatc
     9601 gagattcggg ccggacagca cgtaataata aacaccgtca ctcaccgcgg ggatccagtc
     9661 gttctggtaa ttcgcatgct ggtaaatgat atttccccag cggttgtaaa caatgagatc
     9721 cgaatccgga tgtctctgca ggttcgtgaa cacgagcgca tcgttctttc cgtctccgtt
     9781 cggcgtgacg acgttcgggg cgatgacggg aatatccgtc tggacgagat aaagctgcgt
     9841 aagcgacgcc gtgcagccgc ttcccaccgt caccgtcagc gttaccgtgt acagcgaatc
     9901 atgcgcatac gtatgcgtgg gattctgcag cactgatgtt tgtccgtcac cgaaatccca
     9961 cagccagctg gtcgcgccgg gtgtgttgtc ggtgaactgg acgttgacgc ccatcggctg
    10021 aaccggtggc gggttcatcg tgaagctgac cgccggtgtg gaaatgatat cgacgatcac
    10081 attcgccgac gtggccgcag gcggcgagcc gttgtccgtt ccggtgatcg tgatcgtgtt
    10141 gtaaccggcg ttcgctgcgt tggcaatgag cagcgcggtg atcacggcag gatttcccga
    10201 agtgttcgaa gtgacggtaa gtccctgcac ggacgaagtg atcgttgccg ttgtattctg
    10261 accgttctcg ggcgaaagga aggtggcgga aatgctggtc gtatcaccga tgcagagaac
    10321 gatcgtatcg catacatcgg cagagttgat gatcggcgga aggttgttcc cgcttccgtt
    10381 attgcagacg ttaaaataaa atgtctggtt gtcgagccag ctcacctggt cgggattgcc
    10441 gaagggaccg tcgtaatttg tcccgggcga atcgaactgc ccgatctgga tataatccgt
    10501 tccgttcccg cgattcacgc ccactgtcgc cggcgagccg ccgaaaccac ctgatccgcc
    10561 ggaagcatct cccgtcgtcc attgcatatc gccgtagcag aaattcacat tgttcccgga
    10621 gggaaggatc gggtcgctcc cgtcggtaat gatgagctgg aaatcattca ccttatcggc
    10681 gtacgcgttg aaataaccca ccgttttcca gcggaccacc atgtatgtcg gcgtcacctt
    10741 gtaatacacg agcccgctct gcgcgttgcg cgtatcgaca tcgccccaga aaggcgcgat
    10801 catcacgaaa cccggatcgg gaaatgaatt ggcggtaaac gttccgtacg aagtgaggaa
    10861 cgaaatattc ccgttgttgt tgatgaagca gctgttcatc gactgcccgt aaaagcagaa
    10921 attgaacggc agcgtaatct gtgcagagga accgtcatcg ttgcggtaat cgggaccggt
    10981 ggcaaactgg aacggcacaa cctggaacga cgcatcgatc gggatcatgc aattgcagag
    11041 aacgctcgat tgcgggtccg ccggttcgat cctcggctgt acgggcatgg acgaagtatc
    11101 gccattcacg atcagcagcg gcgctttcgg aagctgcccg ctttctttga gcttttcata
    11161 ttcaggatta cccgagcctt gtattctgac cggggactgg gcatgcagaa atgtgaacag
    11221 gaaaacggga atgactggaa gaattatccg ccgtaatgca ttcattggtt ggatcacttt
    11281 attatataac gtaacgggga actcaaagtt acgcaataca atttcgcctg ctaccgtata
    11341 acgctgacag atccgatata actgtgcgta gtgccgtcaa tgccgccgac aaaaattttg
    11401 tagacatacg tatccgacat cacgtactgg tcattgagcc ggccgtccca tcccttgtac
    11461 ggatcctgcg aatggaacac aaggttgccc cagcggtcga aaaccatgaa gtcgtattga
    11521 ttgacataag taacgtcgct gaacaccggc gtgaatacat cgttgcggtc gttcccgttc
    11581 ggcgtgaatg cattcgggat ccagatgaga agctcgggag aaatcgtcag cgtggcccat
    11641 gccgtatcgg gacaggcgcc gcctgcagca gaaacaacga gcatgacatt gtacgaacct
    11701 gtatcaggat aaacgtaatt cggattctgc gaagtcgagt tagctccgct cacaccgaaa
    11761 tcccaatacc attcctgcgg accggccgtc gactgatcgg taaaataaat cgtgggatcc
    11821 gacatcgttg ccggttgcgg tgtaagtgag aacgcagcct gcggcccggg aataacgtga
    11881 atgtaattgt gaatggaatc cgtatacgaa cacccctgct gcgacgtcac ggtcagcacg
    11941 acggaataat caccctggtt cgcgaaacaa tggttcggat ccgggatcgt gtcgctgccg
    12001 ccgtcaggat attgccagtt ccagtgcgca ggaacactgt tctgcaccgt cgactggtcc
    12061 gccagatcga cacaaagcgg tgcgcagcca atgagcggtc cgcccgagaa ctgcacctgc
    12121 ggcaaataga acacttgcgc attgacgatc gcggtgtctt tgcagcccag cgaatcaata
    12181 acggtaagcg taacaggata tgctccgggt gtgttgtaca cccattgcgg cgactggata
    12241 tccgatgtgt cgttcgtctg cgcaggcaac ccgaaatccc agtgatagga agtgatcggc
    12301 ccgttcacgg tcgtcgatgc gtcgttgaac tgcatcggca ggccgggaca cacgcccgcg
    12361 cttgtgaagg aagctgtcgg tcccgcaatg acgttgatga gaacatttcc gttcgttgtc
    12421 tgcggcggcg ttccattgtc ggtaccggat aatgtcacgg tattcaaccc gatgttcgag
    12481 gtcatgccca caaaaattgc gttgaagctg gccgggtttc cgggtgtgct caccaacgtg
    12541 gtgagcccgg ttccgggggt gctcgcagtg atcgtcgtcg tctggttatt ttcaggtgaa
    12601 aggaattgcg ccgtaatggg aagcgtatca ccgacgcaga gcgtgagcgt atcgcatacc
    12661 agcgccgcgt tcatgatcgg gggaagattt ccgcccccgc ccgtgctgca cacgtcgagg
    12721 aaaaactgct tgttgtccag ccagctgatc tggctcgggg gaccgaacgg cccgttgtac
    12781 gtcgtacctg cctggtcaaa tgtcccgatc tggatgtagt tcacaccatc gcccatattc
    12841 gcaccgaccg tcgctgccgt accgccgaaa ccgttggagc cgccggaagc gtcacccgtg
    12901 gtccactgca tatcgccgta acaaaaaccg cagttgcttc ccgggggcag cagcggatcc
    12961 gttccatccg tgatgatcag ctgaaaatcg ttcagcttat ccacgtgctg cgaataatat
    13021 cccacgttct gccagtgcac gatcagcgcc gtcggcgtca ctttgtaata aacgatgccg
    13081 ctcgtcaggt tacgcgtatc gacatccgcc cagaagggcg cgatcatcgt gtaaaaactg
    13141 ctgggaaatg cactggcgga aaacgttccg tacggagaac cgaacgaaat atttccattg
    13201 ttgttgatgt atacctgcga ctgcacgttt ccataaaagc agaaaacgaa cggaagattg
    13261 atcaccggcg atgatccgtc gtcattccgg taatccggcg ccgttccgaa ctggaaaggc
    13321 accacctgga aagtcgcatc gatcgggatc atgcagttgc agttggcagg aacagtctgc
    13381 gggttcgtgg acggaggctg aacaatcgtc ttcagcggct ggggatcctg gccctgcttc
    13441 atcacgtaaa ggcccgtgtt cagcttgcct tccgccttga gctggtccca ttgctggggc
    13501 gtgatctcca tcggagcatt gacagcagta tcgttctgtg cgaatgaata cacacgcaca
    13561 gaaagcagga atatgaatac tgcgatcctt ttcattgata catgcattgc gcaggccccg
    13621 gagtgatccg atagatgttt gccatatcca attgcctgca ggtcaattta atttacaaaa
    13681 taacaaaaaa aacttctcgg atcggaatta aaatcagtta gctcatttaa caaactgaat
    13741 tacagcagat taacgagcca agcacgatat gtgatcctcc
//
LOCUS       DASTSF010000593         4762 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_582959,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000593 DASTSF010000000
VERSION     DASTSF010000593.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4762
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_582959"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            109..1125
                     /locus_tag="VFU15_16780"
     CDS             109..1125
                     /locus_tag="VFU15_16780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019944086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM/SPASM domain-containing protein"
                     /protein_id="HEU4719502.1"
                     /translation="MSTGFRILVRSFTPARAWNAVKLWSSYRYSRITKKPVQWGLPAS
                     VSIEPTTSCNLRCPECPSGLRSFSRPVGMLEEKFFRKMIDGIAPHVSYLTFYFQGEPY
                     LNPQFTEMVKYAAQKRIYTSTSTNAHFLDDETAKRTVESGLDRLIISIDGTTEETYRS
                     YRIGGKLSKVIEGTKNIVKWKKELRSSTPHIVFQFLVVRPNEHQVKDVKILAEKLGVD
                     EVLYKTAQVYEFEHGNELIPREEKYSRYKQMNDGSWVVKNKMENGCWKMWHSCVITWD
                     GSVVPCCFDKDAAHRMGTLKNFSMEKIWFSEPYRKFRAAILHDRKEIDICRNCSEGSL
                     VWVE"
     gene            complement(1184..4366)
                     /locus_tag="VFU15_16785"
     CDS             complement(1184..4366)
                     /locus_tag="VFU15_16785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009582384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEU4719503.1"
                     /translation="MEYDFHYIEQKWQQAWKERHTYKVENGGPKPKCYVLDMFPYPSG
                     AGLHVGHPLGYIASDIVARYKRLAGYNVLHPMGYDAFGLPAEQYAIQTGQHPAKTTED
                     NIKRYREQMDRIGFSFDWSREVRTSDPGYYKWTQWIFLKLFGSWYNNEKNKAEPVETL
                     IAVFEKEGFRGEEHRVLTGDIEHDVQPFTAEQWKNFTEKEKSDVLMNFRLAYLGEAWV
                     NWCPALGTVLANDEVKDGVSERGGYPVERKRMPQWSMRITSYADRLLNDLDALDWTES
                     MKEAQRNWIGRSEGTSLKFRVPRPGAGQEQADLEIEVFTTRPDTVFGVTFVTLAPEHE
                     LIAQITTAEQKKEVDEYVTMAKNRSERERQADVKKVTGAFTGAYAIHPFTNEKIPVWT
                     GDYVLAGYGTGAVMGVPAHDSRDFAFAKHFGLPIKQVIEVSTAGLNKHSSAGSNEHNS
                     ASSNEHNSASSNEHNSASSNEHNSASSNEHNSASSNEHTSDSSDKHNSDSSDKHLSVS
                     SSASREQTESYDAKEGKCINSGFLNGLDVKDAVKRAIAEIEKRGLGKGKVNYRLRDAA
                     FGRQRYWGEPIPVYYKDGIPHPLPENELPLVLPEIDKFQPTETGEPPLGRAKDWKYKG
                     QYEYELTTMPGWAGSSWYYLRYMDPHNADEFVSKEAVDYWKNVDLYIGGAEHATGHLL
                     YVRFWTKFLFDTGKIPVQEPAQKLVNQGMIQGVSAFVYRLNLRIIDQPTDTQINLPPV
                     FVSCNKYKILCDRTHPEYSNAYSEIETVLNQISNELQRQGKLWSLDLSERIFLPPLNV
                     PIDFVENDILNIQLFHKWRPESQNAKFILENGEYICGREVEKMSKSKYNVVTPDDICN
                     DYGADTLRLYEMFLGPLEQSKPWNTNGITGVHGFLKRFWRLFENPGTEKANDAELKIL
                     NRTLKKVKDDIERMSFNTCVSQFMIAVNELTDLKCSKRAILEPLVIALSPFAPHIAEE
                     LWEKLGHSESVTKAEFPAIDEKYLVDDTFDYPVQVNGKVRFTFTASLQKQPKEIESEV
                     LSTDEAKKWLEGKTPKKVIVVPKRIVNIVV"
BASE COUNT          999 a         1288 c         1253 g         1222 t
ORIGIN      
        1 ttcctgtatt ccgaaatgcg cgttacctcg gtgaataatc cccagttcgt gcgcaacata
       61 acggtgaagt aaatccccgg gcagggaaaa gcgtaacttt aaagaacaat gagcaccggc
      121 ttcaggatcc tcgtacgctc attcacgccc gcccgcgcat ggaacgcggt gaagctgtgg
      181 agcagctacc ggtattcacg catcacgaaa aagcccgtgc agtggggact tcccgcaagc
      241 gtttccatcg aaccgacgac atcgtgcaac ctgcgttgcc ccgaatgccc gagcggactg
      301 cgcagcttct cgcgcccggt gggaatgctg gaagaaaaat tcttccgcaa aatgatcgac
      361 gggatcgcgc cgcatgtttc gtacctcacg ttctatttcc agggcgagcc gtacctcaac
      421 ccgcagttca ccgaaatggt gaagtacgca gcgcagaaga ggatctacac gtccacatcc
      481 accaatgcac attttctcga cgacgaaacg gcgaagcgga ccgtggaatc cggcctcgac
      541 aggctcatca tttccattga cgggacgacg gaagaaacct atcgctcgta ccgtatcggc
      601 gggaagctgt cgaaagtgat cgaaggaacg aagaacatcg tgaaatggaa aaaagagctc
      661 cggtcttcca cgccgcacat cgtgttccag tttcttgtgg ttcgcccgaa cgagcaccag
      721 gtgaaggacg tgaaaattct tgcggaaaag ctcggcgtcg atgaggtgct gtacaaaaca
      781 gcgcaggtat acgagttcga gcatggcaac gagctgatcc cgcgcgagga aaaatattcg
      841 cgctataagc agatgaacga cggcagctgg gtggtgaaga acaaaatgga gaacggctgc
      901 tggaagatgt ggcattcctg cgtgatcacg tgggacggaa gcgtggtgcc ctgctgcttc
      961 gataaagacg cggcgcaccg catgggcacg ctgaagaatt tttcaatgga aaagatctgg
     1021 ttcagcgaac cgtaccggaa attccgcgcg gctatacttc acgaccggaa agagatcgat
     1081 atctgcagga attgttccga aggaagcctg gtttgggtgg agtgattttg ccgcgtcagt
     1141 ccgagtgcgt cgaagacgcg tatcgaggac gagcggcaaa agttcaaacc acgatattca
     1201 caatacgttt cggaacgacg atcactttct tcggcgtttt tccttccagc catttttttg
     1261 cttcgtcggt ggaaagcact tcgctctcga tctccttcgg ctgtttctgc agtgatgcgg
     1321 tgaaagtaaa acgcaccttg ccgttcacct gcaccgggta atcgaacgtg tcgtcgacaa
     1381 ggtatttttc atcgattgcc gggaattccg ctttcgtgac gctttccgaa tgtcccagtt
     1441 tttcccacag ttcttccgcg atgtgcggcg cgaacggaga caatgcgatc acgagtggtt
     1501 caaggatcgc gcgcttgctg catttcaaat ccgtcagctc gttcaccgcg atcatgaact
     1561 ggctcacgca cgtgttgaac gacatgcgct cgatatcgtc cttcactttt ttcagcgtgc
     1621 ggttgaggat tttcagttcc gcgtcgttcg ctttttcggt accggggttt tcaaacaaac
     1681 gccagaaacg cttcaggaac ccatgcacgc ccgtaatgcc gttcgtattc cacggcttcg
     1741 attgctccag cggaccgagg aacatttcgt acaggcggag cgtgtcggcg ccgtagtcgt
     1801 tgcagatatc gtcgggggta acgacgttgt atttcgactt ggacattttt tcgacttcac
     1861 gcccacatat atattctcca ttctcaagaa taaatttcgc attttgagat tcgggcctcc
     1921 acttatgaaa aagttgaata ttcaaaatgt cattttcaac gaaatcaata ggcacgttca
     1981 gagggggaag aaagattctt tcagataaat ctaaactcca aagcttccct tgcctttgca
     2041 gttcattact aatttggttt aaaaccgttt ctatttcaga gtacgcatta gaatattccg
     2101 gatgtgtcct gtcgcataaa attttatact tattgcaact aacgaaaacc ggaggtaaat
     2161 taatttgtgt gtctgtcggt tgatctatta ttcgaagatt caggcgataa acaaacgcac
     2221 tcaccccctg tatcatcccc tggttcacta atttttgcgc tggctcctgc acgggaattt
     2281 tcccggtgtc gaataagaat tttgtccaga agcgcacgta gagcaaatgc ccggttgcgt
     2341 gctccgcgcc gccgatgtag agatcgacat tcttccagta gtccaccgct tccttcgaaa
     2401 cgaattcatc cgcattgtgc ggatccatgt agcgcaggta gtaccaactg ctgccggccc
     2461 agccaggcat tgtcgtcagc tcgtattcgt attgcccttt gtatttccaa tctttcgcgc
     2521 ggcccagcgg cggctcgccc gtttctgtcg gctggaactt atcgatctcc ggcaacacca
     2581 gcggcaattc attctccggc agcggatgcg gaatgccatc cttgtaataa accggtatcg
     2641 gttcgcccca atagcgctgc cttccgaaag ccgcatcgcg caaacggtaa ttcactttcc
     2701 cttttccgag tccgcgcttt tcgatctcgg cgatcgcgcg tttcacggcg tctttcacgt
     2761 cgagaccgtt gaggaaacca gagttgatgc atttgccttc tttggcgtcg tagctctctg
     2821 tctgttctcg actcgcgctc gaactgacag agaggtgttt atccgaactg tcagagttgt
     2881 gtttatccga actgtcagaa gtgtgttcgt tcgaactggc agagttgtgt tcgttcgaac
     2941 tggcggagtt gtgttcgttc gaactggcgg agttgtgttc gttcgaactg gcagaattgt
     3001 gttcgttcga actggcggag ttgtgttcgt tcgaaccggc agagctgtgt ttattcagac
     3061 cggcagtgga cacttcgatc acctgcttta tgggaagccc gaaatgtttc gcaaaggcga
     3121 aatcgcggga atcgtgggcg ggtacgccca tcactgcgcc tgtaccgtag cctgcgagca
     3181 cgtagtcgcc cgtccacaca ggaatttttt cattggtgaa cgggtggatc gcgtacgcgc
     3241 ccgtgaatgc gcctgtcact tttttcacat ccgcctggcg ctcgcgctcg ctgcggttct
     3301 tcgccatcgt cacgtattcg tccacttctt ttttctgctc cgccgtcgtg atctgcgcga
     3361 tcagctcatg ctctggcgcc agcgttacga acgtcacgcc gaaaacggta tcggggcggg
     3421 ttgtgaaaac ttcaatttcc aggtccgcct gttcctggcc ggcgcccgga cgcggaacgc
     3481 ggaactttaa actcgttcct tcagagcgcc cgatccagtt gcgctgcgct tctttcatcg
     3541 attctgtcca gtcgagcgca tcgaggtcat tcagcaaacg gtcagcgtaa gaagtgatgc
     3601 gcatgctcca ctgcggcatg cgttttcttt ccacgggata gccgccgcgt tccgaaacgc
     3661 cgtctttcac ttcgtcgttc gcgaggacgg ttcccagggc ggggcaccag ttcacccacg
     3721 cttcgccgag gtaggcgaga cggaaattca tcagcacatc acttttttct ttttccgtaa
     3781 agttcttcca ctgttccgcg gtaaacggct gcacgtcgtg ctcgatgtcg cccgtcagta
     3841 cgcggtgctc ttcgcctctg aaaccttctt tttcaaaaac ggcgatcagc gtttccaccg
     3901 gctcggcttt gttcttttca ttgttgtacc acgacccgaa cagcttgagg aagatccact
     3961 gcgtccattt gtaatagccc ggatcgctcg tgcgcacctc ccggctccag tcgaacgaga
     4021 agccgatcct gtccatctgc tcgcggtagc gttttatgtt gtcttccgtc gttttcgcgg
     4081 ggtgctgtcc tgtctgtatc gcgtactgtt ccgccggcag cccgaacgcg tcgtagccca
     4141 tcgggtgcag cacgttgtat cccgccaggc gcttgtagcg cgccacgatg tccgatgcaa
     4201 tgtacccgag cgggtggccc acatgcagcc cggcgccgga agggtagggg aacatgtcga
     4261 gcacgtagca tttcggcttc gggccgccgt tctcaacttt gtacgtgtgg cgctctttcc
     4321 atgcctgctg ccatttctgt tctatgtagt ggaaatcgta ttccataatc gtgcgaaaac
     4381 gcgttggtgt ttgcgaacga cgaaattacg caaaagcgtt gtagtcgccg gctgccgttt
     4441 cgcgctgtgc gctgtagtcc tcgcacgcca ctgtcggcct gagcgcgagt cgaaggccag
     4501 tggcgtgctg cgggctgccg cttccatcgc gggcaggggc gggttggcag cttgcccggc
     4561 cttcttcgca tccccaaaaa actaagcagc aggttgcaga tttaaaattg cttgattaac
     4621 ggttggtgtt tggcgcagtg aatgagttcg gagagctgaa ctgcctgccg taaaatccgt
     4681 gttacaggtg gttttttctt tctgtcgtta ttgctttttt atttcataac ataattctac
     4741 catcccgttt ttatacgctt tc
//
LOCUS       DASTSF010000594         4255 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_908237,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000594 DASTSF010000000
VERSION     DASTSF010000594.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4255
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_908237"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(127..531)
                     /locus_tag="VFU15_16790"
     CDS             complement(127..531)
                     /locus_tag="VFU15_16790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719504.1"
                     /translation="MKNLILILAVTAGAFLSSCKKNKDAHIPPDLEFKTGGNYTSGDR
                     SVTHGDSVLVGITITKKEDDLKTFNISYAYDGASTTTSHYNYVMTSAEYGGYDHDFWI
                     VTRNQAGTEKWVFTVTDRDGNLAQKAISLTVQ"
     gene            complement(575..2827)
                     /locus_tag="VFU15_16795"
     CDS             complement(575..2827)
                     /locus_tag="VFU15_16795"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU4719505.1"
                     /translation="MKNPKLKRLALSVVFCMTGMLLHAQLHTLKGKVTDKTTGLPVPY
                     TSVYLPDLTRGTTADINGDFTLAGLPGNFQRITLSSLGYEAYATTVFFTDSVTSFTFT
                     LDPGNNEMEEVVVVGTQSNAKNQSATTVTTLNQHAMRSRGALSISDAVAKLPGVTQLT
                     TGAGISKPVIRGLYGNRIQTVVMGMRFDNQQWQDEHGLGIQDIGVDRVEVIKGPAALM
                     YGSEAMGGVINIIEEKPADVDSLRGDASLRLFSNTFGTGLDAGVKKSTASHWWRVRLG
                     YESQADYSDGHNKRILNSRFDGYVAKATYGFTRRNWVCANNYLFSKSDFGFIMDTANA
                     LVSDARVSRDFSMPHHTVYINLFTTQNTWFGNNGNIFRIIGGAHVNRRMEQEGGNQIS
                     LDMQLITVALHAQYEKHFSDSLSLVTGIQSQYQDNKNFGVRIIVPDANLAEGSYYAYL
                     KRVGKIAAIEAGLRFDTRYIRTFSTGGLDTGFYGIPLINKLYTAVNGSVGSCFRVSEE
                     FHIRANITSGYRTPNLAELSSNGVHEGTLRYEIGNANMKIEQNVCGDLGLTFEKKDFE
                     FTASAYYNRFLNYIYLTPTDGDWYGFRIYRFVQTNATLRGGETSVDWSPSFAEWLDVS
                     AAYMMIRATTDEGNYLPFIPADRIDGSVKFELGDHGKWNDVYLRLGTQYCFPQDHPAQ
                     FETATSDYMLFDAGAGTVLKLKGGNSFTMDIVGNNLLNKVYYDHLSRFKDFGIYNIGR
                     NISLNLRYEW"
     gene            complement(2874..3305)
                     /locus_tag="VFU15_16800"
     CDS             complement(2874..3305)
                     /locus_tag="VFU15_16800"
                     /inference="COORDINATES: protein motif:HMM:NF025032.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HEU4719506.1"
                     /translation="MKKYILAGFIAFAAIAFTASSCKKYEDGPAISLLTKKMRLCNDW
                     VVDKYLENGTDETNGTDMTSFMQSVITEWSIKKDNSYTITGAGSESGTWSLGEDKDDV
                     TFTPSSGTARTFRILMLKSKELRLRQTNTNGTYNYLFLKQK"
     gene            complement(3422..3949)
                     /locus_tag="VFU15_16805"
     CDS             complement(3422..3949)
                     /locus_tag="VFU15_16805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719507.1"
                     /translation="MKPEHKYRIVALLLAGIVFMSSSGFLLLAFLQTGIHRMYEYSRI
                     GSEPSRTLCLGTQEFKSLGWIGEEDFIYKGKVYDMQSVSCANGKILVKCSPDNEETGI
                     RKCVAQNLGADNSSSQQKLPVKDFFKIIPLFPVTGETGTTAVAVVTGFSYAPVELVQL
                     HGAYTGIAVPPPDAA"
     gene            complement(3988..>4255)
                     /locus_tag="VFU15_16810"
     CDS             complement(3988..>4255)
                     /locus_tag="VFU15_16810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719508.1"
                     /translation="APGSQFVVGIIADKTTDDLKLLYTEVAFDGANTTSLVSRVWLAP
                     DERSHFERDITVTLRSQAGTERWVFNVNDANGRISKKEIRVTVQ"
BASE COUNT          952 a         1115 c         1173 g         1015 t
ORIGIN      
        1 tatcccggca gcaccagctt caacacgacg ttcctcatcg gcgtgcaggc gttcttccac
       61 cacgactaag tcagaaaaaa aaggaataaa aaagaacgga gcagcaaccg gctccgtttt
      121 tttgttctat tgaaccgtca gcgagatcgc tttctgcgcg aggttcccgt cgcggtcggt
      181 gacagtgaac acccattttt ccgttccggc ctggttgcgc gtcacgatcc agaaatcatg
      241 gtcatagccg ccatattccg ccgatgtcat gacgtaattg taatgcgagg tcgtcgttga
      301 cgcaccgtcg taggcatacg agatgttgaa cgttttcagg tcgtcctcct ttttcgtgat
      361 cgtgatgccg acgagcacgc tgtcgccgtg cgtgacggaa cggtcgccgc tggtgtaatt
      421 gccgcctgtt ttgaattcga gatcaggagg aatgtgcgcg tccttgttct ttttgcagga
      481 ggacaggaag gccccggccg ttaccgcgag aattaagatg agatttttca tgttcatgat
      541 tttatctccg cttccggagg attttttcag acaattacca ttcgtatctg aggttgagac
      601 tgatgttccg gccgatgttg taaataccga aatccttgaa acgggagagg tgatcgtaat
      661 acactttgtt cagcaggttg ttgcccacga tatccatggt gaagctgttc ccgcctttca
      721 gcttcagcac ggttcccgct cctgcgtcga acagcatgta atccgacgta gctgtttcga
      781 actgcgccgg gtgatcctgc gggaaacaat attgcgttcc cagccggaga taaacgtcat
      841 tccattttcc gtgatcaccg agctcgaact tcaccgatcc gtcgatgcgg tcggcgggaa
      901 tgaacggaag gtaatttcct tcatccgtgg ttgcgcggat catcatgtac gccgcagaaa
      961 catcgagcca ttccgcgaac gaaggcgacc agtcgacact cgtttctccc ccgcgcagcg
     1021 tggcgttcgt ctgtacaaaa cggtaaatgc ggaaaccata ccagtctcca tccgtcggtg
     1081 taaggtaaat gtaattgagg aagcggttgt aatacgcgga agccgtgaac tcgaaatcct
     1141 ttttttcgaa tgtgagcccg agatcgccgc aaacgttctg ctcgatcttc atgttcgcgt
     1201 tgccgatctc gtaacgcagc gttccttcat gaaccccgtt cgaggaaagc tcggcaaggt
     1261 tcggtgtgcg gtaaccgctg gtgatgttcg cgcggatgtg aaattcttcg gagacgcgga
     1321 aacagcttcc gacagaacca ttcaccgccg tgtacaattt attgatgagc ggaatgccgt
     1381 agaaaccggt gtcgagtccg cccgtggaaa acgtccggat gtaacgcgtg tcgaaacgaa
     1441 gtcctgcctc gatcgccgca attttcccga cacgtttcag gtaggcgtag taggagcctt
     1501 ccgcgaggtt cgcatccgga acgataatgc gcacgccgaa gttcttgttg tcctggtatt
     1561 gcgactgtat gccggtgacg agcgaaagcg aatccgaaaa atgtttttca tactgcgcat
     1621 gaagcgcgac ggtgatcagc tgcatgtcga gcgagatctg gttaccgccc tcctgctcca
     1681 tccggcggtt gacgtgcgcc ccgccgatga tgcggaaaat attcccgttg ttgccgaacc
     1741 acgtgttctg cgtggtgaac agattgatgt aaacggtgtg atgcggcatc gaaaaatccc
     1801 ggctcacgcg tgcatcgctc acgagcgcat ttgccgtgtc catgatgaag ccgaaatcgc
     1861 ttttggaaaa aagataattg ttcgcgcaca cccagttcct gcgtgtgaag ccgtacgtcg
     1921 cttttgcaac gtagccgtcg aaacgcgaat tcagtatccg cttgttgtgg ccgtcgctgt
     1981 aatccgcctg cgattcataa ccgagacgga cgcgccacca atgggaagcg gtggattttt
     2041 tcacgccggc atcgagccct gttccgaacg tgttgctgaa caaacgcagg gaagcatcac
     2101 cgcgcagcga atcgacgtcg gccggttttt cttcaatgat gttgatgacg ccgcccatcg
     2161 cttccgaacc gtacatgagc gcggccggac ctttgatcac ttccacgcgg tcgacgccga
     2221 tgtcctgtat gccgagccca tgctcgtcct gccactgctg gttgtcgaaa cgcataccca
     2281 tcaccaccgt ctggatgcgg ttgccgtaca gtccgcggat aacgggtttc gaaattcccg
     2341 cgcccgtggt gagctgcgtt acgccgggta gcttcgccac tgcatcgctg atgctgagcg
     2401 cgccgcggct gcgcatggcg tgctggttga gcgtggtcac ggttgtcgca gactggttct
     2461 tcgcattcga ctgcgtaccg accacgacga cttcctccat ttcgttgttc ccgggatcaa
     2521 gcgtgaacgt aaacgacgta acggaatccg tgaagaaaac agtggttgca tacgcttcgt
     2581 agccgaggct cgacaacgtg atccgctgaa aattgccggg aaggccggcc agcgtaaaat
     2641 cgccgttgat gtcggcagtc gttccgcgcg tgagatcggg aagatagacc gacgtgtacg
     2701 gcacaggaag tcccgtcgtc ttatcggtga cttttccttt cagcgtatgc agctgggcat
     2761 gaagcagcat ccccgtcatg cagaacacaa cagaaagagc aagacgttta agttttggat
     2821 ttttcatttt gaaaaggaag tggcgtgaag acaatttcac gccacttccc ggattatttc
     2881 tgcttgagga aaaggtaatt gtaggttccg ttggtattcg tctggcggag acgcagctct
     2941 ttcgacttca gcataagaat ccggaaggtt cttgctgttc cgctcgaagg cgtgaacgtt
     3001 acatcgtcct tgtcttcgcc gagcgaccag gtgccgcttt cagaaccggc gcccgtgatg
     3061 gtgtagctgt tgtctttttt gatggaccat tcggtaatga ccgactgcat gaacgaggtc
     3121 atgtcggttc cgttcgtttc atcggtcccg ttctccaggt atttgtcgac gacccagtcg
     3181 ttgcacaggc gcattttttt ggtcaggagg ctgatcgcag ggccgtcctc gtatttttta
     3241 caggaggaag ccgtaaatgc aatagcagca aaggcgataa agcccgcaag aatgtacttt
     3301 ttcattgtct ggtttttgga aagtttaaaa gaaaggaaga cggaaataca cgaaacacgc
     3361 ccgggcatcc gccttttatg gcccggaacg aaagactttc aggaaagaaa gaggaaatgc
     3421 gttacgcagc gtccggggga ggaactgcaa tgccggtata agcgccgtga agctgcacca
     3481 gctcaacggg agcgtaggaa aacccggtaa cgacggcaac agccgtggta cctgtttcac
     3541 ccgtaaccgg aaagagagga atgatcttga aaaaatcctt tacaggaagc ttttgctgtg
     3601 aagagctgtt gtcggcgccg aggttctgcg cgacacattt gcggatgccg gtttcttcgt
     3661 tgtcaggaga acatttcacg aggatctttc cgtttgcaca ggaaacagac tgcatgtcgt
     3721 agacttttcc tttgtaaatg aaatcctcct cgcctatcca tccgagcgat ttgaattcct
     3781 gcgtgcccag gcacaacgtg cgcgaaggct cgctgccgat acgggaatat tcatacatgc
     3841 ggtgaatacc ggtctgcagg aacgccagca gcaggaagcc gctgctgctc atgaaaacaa
     3901 tccctgcgag aagcagggcg acgatccggt atttatgctc gggtttcaag aaatacaaag
     3961 ataatcacga aacaggcgaa aggcaagtta ctgcaccgtc acacgtattt ccttcttcga
     4021 aatcctgccg ttggcgtcgt tgacgttgaa gacccagcgc tccgtcccgg cctggctgcg
     4081 cagcgtaacg gtgatgtcgc gctcgaagtg gctgcgctcg tccggcgcca gccagacgcg
     4141 ggaaacaagg gaagtggtgt tcgcgccgtc aaatgcaact tccgtgtaaa gcagcttgag
     4201 atcatccgtg gttttatcgg caatgatgcc caccacgaac tgcgacccgg gcgct
//
LOCUS       DASTSF010000595         2753 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1460931,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000595 DASTSF010000000
VERSION     DASTSF010000595.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2753
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1460931"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            89..1312
                     /locus_tag="VFU15_16815"
     CDS             89..1312
                     /locus_tag="VFU15_16815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017259792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEU4719509.1"
                     /translation="MPHISRKAENMPQSPIRKLVPFAEKAKKEGRKIYHLNIGQPDIE
                     TPEVMLNAIKNSGLKVIEYSHSAGFESYRRKLAAYYRKFDMPVETEDIIITTGGSEAI
                     EIAMMTCFNPGDEIIIPEPFYANYNGFSCAADLVVKPIRSYIETGFALPPIADFEKMI
                     TPKTRGIMICNPGNPTGYLYTKDELEALKTLVKKYDLFLLSDEVYREFCYDGKKYTSV
                     MHLSGIEHNVVLLDSISKRYSACGARIGAMISKNKDVMAAALKFAQARLSPPTFGQIG
                     AEAALETPQSYFDAVTKEYTARRDFVIDAINKMEGAFCPKPSGAFYCIARLPIDNADK
                     FCQWLLESFSHNGQTVMLAPATGFYSTPGAGADEVRIAYVLNTHDLKNAMECLDAALK
                     VYPGRSLKKETAQVS"
     gene            complement(1382..>2753)
                     /locus_tag="VFU15_16820"
     CDS             complement(1382..>2753)
                     /locus_tag="VFU15_16820"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEU4719510.1"
                     /translation="TMSGSSANIFAKRVLQVSGDYFILFDYNNGTDRFAVLRYNITTG
                     IVWARDFSGSLSNGGQNTVDMDYDGSSYLYILGDVPDNSSENLWVAKMDLNGNLTWYY
                     EYPEAGDTANEVAWNIHYQNDSGIYIGATTSPGGTDVAGLVVVINNSGAVLNAEKIRA
                     YPFPASPQPLTDRLYVKADNSHVYVVEQIVRSGTPYHLFLAHLNTSLAITKCATYDSP
                     NFWLHPEFRLINGDIVLSGQYYPLSSGNQSYINQFFNASTLSFNNAARYYMTVTNGSE
                     ITLSYDPSNYNIFNTANVGSSTGSIYRIKAHAATGVTQCDSTFAADSLATDTELISFS
                     LDIDTTGALDTLKMKPLSLTTTDTLICDTGSSARMMHLPEAADVPPAGVYPVPFDRQL
                     YVRPGGSGALYAELYSIDGRLLQRQQFDGKDEAILSGDGLDGGIYILNIIYDSGRTET
                     HKVIRQ"
BASE COUNT          660 a          747 c          721 g          625 t
ORIGIN      
        1 cgcccggggc tgacctgcat cacctgcgcg gcaaagcagc aattgcgttc gtcaaaaatt
       61 gcgacattta ctttctcaat cacgcaacat gccacacatt tcccgcaagg cggaaaacat
      121 gccgcagtca ccgatccgga agctcgttcc gttcgcggaa aaagcgaaaa aagaaggtcg
      181 gaagatctat cacctcaaca tcggccagcc cgacatcgaa acgccggaag tgatgctgaa
      241 tgcaataaag aattccggcc tgaaagtgat cgagtattcg cattcggccg gtttcgaaag
      301 ctaccgccgc aagctcgcgg cctattaccg caaattcgat atgccggttg aaaccgaaga
      361 catcattatc accaccggcg gatccgaagc gatcgagatc gcgatgatga cctgcttcaa
      421 ccccggcgac gagatcatta tcccggaacc gttttacgcc aactacaacg ggttttcctg
      481 cgcagccgat ctcgtggtga aaccgatccg ctcctatatc gaaaccgggt ttgcgcttcc
      541 gcccatcgcg gatttcgaaa agatgatcac gccgaaaacg cgcggcatca tgatctgcaa
      601 tcccggcaac cctacgggtt atctctacac gaaagacgag ctcgaagcgc tgaaaacgct
      661 cgtgaaaaaa tacgacctct tccttctttc cgatgaagtg taccgcgagt tctgttacga
      721 cggaaaaaaa tacacgtcgg tcatgcatct ttccggcatt gagcacaacg tcgtgctgct
      781 cgattccatt tccaagcgct acagcgcctg cggcgcacgc atcggcgcga tgatctcgaa
      841 gaataaggac gtgatggcgg ccgcgctgaa attcgcgcag gcgcggctga gtccgccgac
      901 gttcgggcag atcggtgcgg aagctgcgct ggaaacgccg caatcgtatt tcgatgcggt
      961 gacgaaagaa tatacggcgc gacgggattt tgtcatcgac gcgatcaaca aaatggaagg
     1021 cgctttctgc cccaaaccga gcggcgcgtt ttattgcatc gcacgtttgc cgatcgacaa
     1081 tgccgacaaa ttctgccagt ggctcctcga atcgttcagc cacaacggcc agacggtgat
     1141 gctcgcgccc gccaccggct tttattccac gcccggcgca ggagccgatg aagtgcgcat
     1201 cgcttacgtg ctgaacacgc atgatctgaa gaatgcgatg gaatgcctcg atgcagcgct
     1261 gaaagtctac ccgggcagaa gcctgaaaaa agaaacggca caggtatcct gactgttttc
     1321 tgacaaaaca aaaaggacgc gaaatcgcgt ccttttttgt ttttgcattc cgcggctgaa
     1381 cttattgtct tatgacttta tgcgtttccg ttcttccgga atcatagata atgttgagaa
     1441 tgtaaattcc tccgtccagc ccgtctcctg aaagaatggc ctcgtctttt ccgtcgaatt
     1501 gctgccgctg cagcaacctg ccgtctatac tgtacagttc tgcgtatagc gcgccgcttc
     1561 ctcccggacg gacgtaaagc tgccggtcga acggaaccgg gtaaacgccg gccggcggaa
     1621 catctgcagc ttcgggcagg tgcatcattc ttgcgctgct gcctgtatca cagattagcg
     1681 tgtcggttgt ggtcagtgaa agcggtttca ttttgagcgt gtcgagcgcg ccggtggtgt
     1741 cgatgtccag gctgaagctg atcagttccg tgtcggtggc aagagaatcc gcggcaaacg
     1801 tgctgtcgca ctgcgtgaca ccggtcgctg catgcgcctt aatgcgataa atggaacctg
     1861 tgctgctgcc gacattcgct gtgttgaaga tgttgtagtt cgacggatca tagctgagcg
     1921 tgatctcgga cccgttggtc accgtcatgt aataacgtgc cgcgttattg aaggaaagtg
     1981 tcgatgcatt gaagaactgg ttgatatagc tttggttacc tgagctcagc ggatagtatt
     2041 gtccgctcag cacaatatct ccgttgatga gccggaattc gggatgcagc cagaaattgg
     2101 gcgaatcata cgtagcgcat ttcgtaatag cgagggatgt gttcaggtgc gcgaggaata
     2161 aatgatacgg cgtccccgat ctcacgatct gctcgacgac atatacatgg gaattatccg
     2221 ccttgacgta aaggcggtcc gtaagcggct gcggacttgc cgggaaagga taggcgcgga
     2281 tcttttcggc attcagcaca gcgccggagt tgttgatcac gacaacgagc cctgccacat
     2341 ccgttcctcc cggcgaggtc gtcgccccga tgtaaatgcc ggaatcattc tggtaatgaa
     2401 tgttccatgc cacttcgtta gcggtatcgc ctgcttcagg atattcataa taccacgtaa
     2461 ggtttccgtt caggtccatt ttcgccaccc agagattctc gcttgaatta tccggcacat
     2521 caccgagaat atacaggtac gatgatccgt catagtccat atccacggta ttctgccctc
     2581 cattgctcag tgaaccggaa aaatcccgcg cccacacaat gccggtcgtt atgttataac
     2641 gcagcacggc gaaacggtct gtgccattgt tataatcgaa caggatgaaa taatcgccgc
     2701 tgacctgcaa aacgcgtttt gcaaaaatat tggcagacga acccgacatc gtg
//
LOCUS       DASTSF010000596        11344 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1923215,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000596 DASTSF010000000
VERSION     DASTSF010000596.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 11344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11344
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1923215"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            78..386
                     /locus_tag="VFU15_16825"
     CDS             78..386
                     /locus_tag="VFU15_16825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719511.1"
                     /translation="MENKLSRLTLDGETLGVYLKGNNGILENYMFDIQSFDYDGEVYL
                     NASDGTVIFRSDPSNYHESNHVSFEVRFELEKSAGAPVTGRKIECSQRTKQQRFDFVK
                     "
     gene            complement(392..3064)
                     /locus_tag="VFU15_16830"
     CDS             complement(392..3064)
                     /locus_tag="VFU15_16830"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEU4719512.1"
                     /translation="MKFRLLLLALLPAAFIHAQKFLQRPSNGPVTFTQLQKQFDDYKK
                     SGSLKSEKGWKYFARWEEDMLMHTNGRGEKCDPTDYINAAIDMANQKQAASSSQMATS
                     AWYPVGPNYLPGNETGYMENGVGRINCIAFHPTNPSEYFIGVAQGGVWKTTNNGASWT
                     PLTDNLPILRISDIAIDPADPDNTMYISVCDFEYIGFGLYLNGNKRNTHYGLGVYKTT
                     DGGATWQPTGLTFQLTDGDASLIRRVIVDPNNSNIILACGVSGMYRSTDAGATWTHEN
                     NNLYWDLQEDPVSPNIVYAATGWVMTANDGAAGIYKSTDFGQTWAPLNSLIPSTGVVQ
                     RVKLAIAPSDPNYVYALTVDTQNGLYGVYKSTNAGITWTYINPGVNILEAGDGTSSGG
                     QGNYDLGFTVNATDKNILYTGGVNLWSSSDGGNTFDPVSHWTLFYGPTLHGDIHFIER
                     QPSTGNMFVCSDGGIYRTTNIQSQTWLDANNGNPWPTQWTNISNGLTITSFYRLSSSR
                     NAAGRLVAGAQDNATFYYDNGVWNTIFGGDGMDNYLDPANDDMVMGSSQYGNFYLSLD
                     DGMNDWGTTPNINSENAEWTSPLVADYNNNGTLYAGFENVVKSVDGGFTWNQISNFGY
                     VNYPAEIVALAVSNSNPNTLYAGKRVRYEYGEPGAVYKTTNGGGSWTNVTAGLPDSLY
                     YTSIEISENDANTAYVAMAGLSAGNKVFRTIDGGSTWQNISWNLPNIPVNCVKYIPGT
                     GTVMIATDLGVYMLDNINNQWVSRSTGLPNVITTDIDFDVTLNKIYVSTFGRGIWATD
                     LDVFTSSLNVQQPELSLNLFPSPNNGTFTITVPPASNETLSLDVIDISGKTVHSEELS
                     GQSSYTISLDVPSGMYFAKVRGKTLSGVKSFVVQ"
     gene            3222..4571
                     /locus_tag="VFU15_16835"
     CDS             3222..4571
                     /locus_tag="VFU15_16835"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEU4719513.1"
                     /translation="MRILGYVFYRYLWISFNTLLFYFYRFSPRRHTSGMERIPTDRPV
                     IFCSNHPNAFMDALMLGSAVKRRTWFLARSDVFRKKALAKFLSFIGIIPIYRLLEGAE
                     NLSKNDETFDKCSAMLEENKAILIFSEGLCVQERRLRKLKKGTARIALGSEAKNNFNL
                     NLTIIPAGINYSSTPWKFRKPLYISLGEPFAVKDYEALYRESPARAMNQFTRDLEDRM
                     REQLVIIEHKENDELVAQLEEMFMRKWTVEKYGDPRNQADTYHTSKAIAHFVNETTKR
                     EPEKIAALRDKAQTYFSKLEELRLRDWLVTDTKRTGGIIAGVLLALLTLPFWLFGVIT
                     NYVPYKIPWLIQKKIVKNIEWSASVNGTIAVYLWQFYWLLQSLVVALVFRNWYVLGAF
                     MIAVPACGMIAQWNSVRLKKLFGRIRYSNLPANERKDLAEMRSEIEREGEELKGLKV"
     gene            complement(4639..5703)
                     /locus_tag="VFU15_16840"
     CDS             complement(4639..5703)
                     /locus_tag="VFU15_16840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014202229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU4719514.1"
                     /translation="MISKPIISGIQQVGIGIPDVHAAFKWYRRHFGMDVPVFEEAAEA
                     NLMLPYTGGKGHSRHAILAINMKGGGGFEIWQYTSRTPVPVPFEIQLGDIGHFVTRMK
                     SSDVKGSYEFLESKGAEILNGLTSSPDGAPNFFVRDPWGNIFEIVPGDDWFAKGKQLT
                     GGPAGALLGVTDMERSVKFYGEILGYDTVVYDKTGVFDDLKCLPGGNAEVRRVLLKHS
                     KPRAGAFSRLLGSSRIELVKVNGRTPRKIFEGRWWGDRGFIHLCFDIVGMQEMKKLCE
                     SKGHPFTVDSANSFDMGEAAGHFSYIEDPDGTLIEFVETHKVPVLKKLGWYLNLKKRP
                     ADKPLPDWMLKSMGLNRVKD"
     gene            5866..6612
                     /locus_tag="VFU15_16845"
     CDS             5866..6612
                     /locus_tag="VFU15_16845"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU4719515.1"
                     /translation="MSLLRSAFQKITGNEFTDASDEFLNWLLASCPGMQHRGNILCFG
                     QAIQNLPSEDPIVEIGAHAGLSANIICYLLKKHGRKNPVISIDPWIVRGWDDGKENVP
                     EEYLFSLGSNPHVKRSDFAEFMMQSYIRNVKFFSAENLPFAFRMTSDTFFEKWNSGAI
                     GKDIMGREMNSGGRISFVYIDGNHDYDFAKRDFVNADRHLVPGGFVLFDDSQRGSPFG
                     CAKVAEEVKADERYTLVAENPNLLFRKSRG"
     gene            6676..7500
                     /locus_tag="VFU15_16850"
     CDS             6676..7500
                     /locus_tag="VFU15_16850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014397706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU4719516.1"
                     /translation="MTDMTFKNKTIFITGGSRGIGKAIALRLAREGANIVIAAKTTEP
                     HPKLEGTIFTAAEEIEKAGGKCLPVKCDIREEEQVVSAVADAVKNFGGIDVLINNASA
                     INLSPVSLLPLKQYDLMQDINTRGTYLVSRHCIPHLKKSSGAHILTLSPPLSLDKKWF
                     SHFAPYTISKFGMSMLTMGMAEELRKDNIAVNSLWPRTTIATAAVKNILGGDELMKKS
                     RTPEIVADAAFFVLSETSAVTGNFFIDDEVLAKNGITSLSHYAVDGAQELMPDIFL"
     gene            8114..>11344
                     /locus_tag="VFU15_16855"
     CDS             8114..>11344
                     /locus_tag="VFU15_16855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719517.1"
                     /translation="MTRSAFSSVYTPVSGSATALTFSSTDDGTTMIPLPFDFNYLGTT
                     FVNTSNYLAVCTNGWASFSTSSIAPSTTQVNTNLYAVSSPLNTLAPWWDDLNVGVVGT
                     NPAGGASWEVTGSAPNRVMTVQWVASSFYTTTGGQPRQINFQVKLYETTNVIEFWYGA
                     ATGAVTNTSESASIGLSDATGGNGHYLDGVTGSCYTGNADMQSDRFPTYNLRFTPGAP
                     TPLAAGTYNVGVGQTYINLNEAAADVNQRGVSGPVVFNLVDAQYDATAANGGNFFPIL
                     IGPISGTSAVNTVTFTKSSTAAVIRYGGASGTSGSIVNQASTTALASNVDPIIGLVGA
                     DYVTLNNLDIRGNVGNQLADHGLGVYNSSATDGAQHNTFTNISVTMNRANTGSVGIRQ
                     SSVTTATASTGANSNNIYKGLTIKNCFVGITLSGTSGFPDDACEIGTNACGTFNVIGD
                     PLTANDIGNNGGSVWGIKTTNESNVKIYNNVVVNVTGTNANPVDAIFVDNAGVSATVS
                     AGTCMVYNNNITAITSTSTGTSVVRGIRCNLNANAGSVSEIYNNTVSGLNSASTFTSS
                     RRVIGISVQDAGGGAGATHNVDFNSVRIAPAVANCNNTCFEIGTASGPVMNVRNNIFA
                     NFTGAQATVKHYCWVSTSATSNGNAGSVSNYNDLYVNNTTNGFTALGNTTDYATLANW
                     QAITSTPDLNSIAVDPQFSSAIDLHTSAAALDNAGNNTGIPWVTVDIDCQARPQPAAT
                     NFDIGADEITGCSGMPSAGTAGGNPASVCGSGTFTIQTAGASTGIGITYQWQESATPG
                     GPYTNCTTGSGINTSTYTTASISSTMYYVCLVTCSNSGLSATTNEVVATVNASPTVTI
                     TPNPLTICAGGAGASLTASGANTYTWAPSTGLSATTGSTVTANPASSITYTITGTAAN
                     GCTSASTVPVTVNEVPSLTSPTATPATVCSGANSQLLANGGLTNAYTISNITYAPVST
                     PGSGVTTLANNGTAVTALNAGTLDDGDWVNQVIPFNFTYFGTIYTSFALSTNGFICLG
                     SGAPNTYTSYNNAMPSVAMGRPAIGPVYADLDFRTAGTINYFTSGSA"
BASE COUNT         2622 a         3091 c         3007 g         2624 t
ORIGIN      
        1 aagtgtagtg aaccggtgtc acaaaaaaag acgcgcggat aaaaaacaat gatgaaccgt
       61 tcggcgcttc gggagttgtg gaaaacaaac tttcgcgttt gacactggat ggagaaacgc
      121 tgggtgttta cctcaaagga aataatggta tcctggaaaa ctacatgttc gacattcaga
      181 gttttgacta tgatggtgaa gtttatctta atgcaagcga tggtaccgtt atttttaggt
      241 ccgatccatc gaactatcac gaatcaaacc atgtgagttt cgaagtgcgc ttcgaactcg
      301 agaaatcggc aggtgctcct gtaactggta gaaagatcga atgtagccag agaactaaac
      361 agcagcggtt cgattttgtg aaataaaatc atcactgcac cacgaaactc ttcacgccgc
      421 tcaatgtttt tccgcgcact ttcgcaaaat acatcccgga aggaacatcc agcgagatcg
      481 tgtagctgct ttgcccggat aattcttcgg agtggacggt cttgcccgaa atgtcgatca
      541 cgtcgagcga caaggtttca ttgctggccg gcggaacggt gatcgtgaac gtgccgttgt
      601 tcggtgaagg gaaaagattc agcgaaagct ccggctgctg cacattcagc gaagaagtaa
      661 atacgtcgag gtctgttgcc cagatgccgc ggccgaaggt gctcacgtaa attttattca
      721 gcgtaacgtc gaagtcaatg tcggttgtga tgacattggg aagcccggtg ctgcgcgaca
      781 cccactggtt gttgatgttg tcgagcatgt agacgccgag gtcggtggcg atcatcaccg
      841 tgcccgtgcc cggaatatat ttcacgcagt tcacgggaat gttcggcagg ttccaggaaa
      901 tattctgcca cgtgctgccc ccgtcgatcg tgcggaaaac tttattgccc gcgctcagcc
      961 cggccatcgc gacgtacgcg gtgttcgcgt cattctcgct gatctcgatg ctggtgtaat
     1021 acagcgaatc gggaagcccg gccgtaacgt tcgtccagct tccgcccccg ttcgttgttt
     1081 tgtaaacagc gcccggctcg ccgtattcgt aacgcacgcg cttgccggcg tacaaggtgt
     1141 tcgggttcga attcgaaacc gccagcgcga caatttccgc cgggtaattc acgtacccga
     1201 aattcgagat ctggttccag gtgaatccgc cgtcgacgga tttgacgaca ttctcaaaac
     1261 cggcgtacaa cgttccgttg ttgttgtaat ccgcaaccag cggcgatgtc cattcggcat
     1321 tttcagaatt gatattcggt gtcgtgcccc agtcgttcat gccgtcgtcc agcgagagat
     1381 agaaattacc gtactggctc gatcccatta ccatgtcgtc attggcggga tcgaggtaat
     1441 tgtccatgcc gtcgccgccg aagatcgtgt tccatacgcc gttgtcataa tagaacgtgg
     1501 cgttgtcctg cgcgcccgcg accagtcgtc ccgctgcatt gcgcgagctc gacaagcggt
     1561 aaaaagaagt gatcgttaac ccgttgctga tgttcgtcca ttgcgtcggc cacgggtttc
     1621 cgttgttcgc atcgagccat gtctgcgact ggatattcgt tgtccggtaa attcctccgt
     1681 cgctgcagac gaacatgttt cctgtcgacg gctgccgctc gatgaaatgg atgtcgccgt
     1741 gcagcgtggg accgtagaac aacgtccagt ggctgaccgg atcgaatgtg ttccctccgt
     1801 cggaagagct ccacaggttc acaccaccgg tgtaaagaat gtttttgtcc gttgcattta
     1861 cggtgaaacc gagatcgtaa ttaccctgcc cgccggacga tgttccgtcg ccggcttcaa
     1921 gaatgttcac gccgggattg atgtacgtcc acgtgatgcc cgcgttcgtt gatttgtaaa
     1981 cgccgtacaa tccattctgc gtatcgacgg taagcgcgta aacgtaattc ggatcggaag
     2041 gagcgatcgc gagcttcacg cgctgcacga caccggttga gggaataagt gaattcagcg
     2101 gcgcccacgt ttgtccgaaa tcggtcgatt tgtaaatgcc cgctgcgccg tcgttcgctg
     2161 tcatcaccca gccggtggca gcgtaaacga tgttcggcga aacaggatct tcctgcaggt
     2221 cccagtaaag gttgttgttc tcgtgcgtcc acgttgcgcc ggcgtccgtc gacctgtaca
     2281 tgccactcac gccgcacgcg aggatgatgt tgctgttgtt cggatcgacg atgacgcggc
     2341 ggatgagcga agcgtctccg tcagtgagct ggaatgtgag gccggtgggt tgccacgtcg
     2401 caccgccgtc ggttgttttg taaacgccga gaccgtaatg cgtgttgcgc ttgttgccgt
     2461 tgaggtaaag tccgaacccg atgtattcga aatcgcagac ggaaatgtac atcgtgttgt
     2521 cgggatcggc cggatcgatg gcgatatcgc tgatgcggag aatgggaagg ttgtcggtga
     2581 gcggtgtcca gcttgcgccg ttgttcgtcg ttttccacac gccgccctgc gccacgccga
     2641 tgaaatattc cgaaggattc gtcggatgga acgcgatgca gttgatgcgc ccgacgccat
     2701 tttccatgta gccggtttca ttgccgggaa ggtaattcgg tccgaccggg taccacgccg
     2761 acgttgccat ctgcgaggaa gaagccgcct gtttctggtt ggccatgtcg atcgcggcat
     2821 tgatgtaatc cgtaggatcg catttttcac cgcgcccgtt cgtgtgcatg agcatgtcct
     2881 cttcccatcg cgcgaaatat ttccatcctt tttcggattt caggctgccc gattttttat
     2941 aatcgtcgaa ttgcttctgc agctgtgtga aagtaacggg accgttcgaa ggccgctgca
     3001 ggaatttctg cgcatggatg aacgctgccg gcagcaacgc aagcagcaaa agacggaatt
     3061 tcatggaagg aggtttttat gtggcaacta aatttacgga attggcacga acgcccggcc
     3121 cccgctttcc cgcttttcaa ctttccaact cccgggcttt ccgacggtga tcacccgatc
     3181 ccgcacattc tcatccgcct cgttatcttt ggcacttcgt tatgcgcatt ctcggctacg
     3241 ttttttaccg ttacctctgg atatcgttca ataccctgct tttttatttc taccgctttt
     3301 cgccccgcag acacacgagc ggaatggagc gtattcccac ggaccgcccc gttattttct
     3361 gctcgaacca cccgaacgcg ttcatggatg cgctcatgct cggttctgcc gtgaagaggc
     3421 gcacgtggtt cctcgcccgg tcggatgtgt tccggaaaaa agcgctcgcg aaatttttgt
     3481 ccttcatcgg catcattccc atttaccgac tgctggaagg cgcggaaaat ctttcgaaga
     3541 acgacgagac gttcgataag tgctccgcga tgctcgagga gaacaaagcc attctgattt
     3601 tttccgaagg actttgcgtg caggaacggc gcctgcgcaa gctgaagaaa ggaaccgccc
     3661 ggatcgcgct cggatcggaa gcgaagaata atttcaacct caacctcacg atcatcccgg
     3721 ccggaattaa ttattcctcc acgccctgga aattccgcaa gccgctttac atcagcctgg
     3781 gcgaaccgtt tgcagtgaaa gattacgaag cgctgtaccg cgaaagcccg gcgcgcgcga
     3841 tgaaccagtt cacccgcgat cttgaagacc gcatgcggga acagctcgtg atcattgaac
     3901 acaaagagaa cgatgagctt gtcgcgcagc tggaggaaat gttcatgcgg aaatggacgg
     3961 tggagaaata cggtgatccg cgcaaccagg ccgacacgta tcacacgtcg aaagccattg
     4021 cgcatttcgt gaatgaaacg acaaaacgcg agccggaaaa gatcgccgcc ctgcgtgata
     4081 aagcgcaaac gtatttttca aagctggaag agctacgcct ccgcgactgg ctcgtgaccg
     4141 acacgaaaag aacgggtgga ataatcgccg gcgtcctgct ggcgctgctc acgcttccgt
     4201 tctggctctt cggcgtgatc acgaattatg ttccgtacaa aatcccctgg ctcatccaga
     4261 aaaagatcgt gaagaacatc gagtggagcg cttctgtcaa cggcacgatc gccgtttacc
     4321 tctggcagtt ttactggctg ctgcaaagtc ttgtcgttgc gcttgtgttc cgcaactggt
     4381 acgtgctggg agcattcatg atcgccgtgc cagcctgcgg catgatcgcg caatggaatt
     4441 ccgtgcggct gaaaaaactc ttcggcagga tccggtattc aaatcttccc gcgaacgaaa
     4501 gaaaggacct cgcggaaatg cgttcggaaa tcgaaaggga aggggaagag ttgaaaggtt
     4561 tgaaagttta aagctggctt agttgagaac cgaaaacgtc cggcgacaaa cgcgaaacga
     4621 aaaactccat gaacccgttt aatccttcac cctgttcagc cccatgctct tcagcatcca
     4681 gtcggggagc ggcttatcgg ccgggcgttt tttgagattg aggtaccagc ctaatttttt
     4741 cagcacggga actttgtgtg tttccacgaa ttcgatgagc gtcccgtcgg gatcttcgat
     4801 gtagctgaaa tggcccgccg cttcgcccat gtcgaagctg ttcgcgctgt cgacggtgaa
     4861 cgggtgtcct ttcgattcgc atagcttctt catttcctgc atgcctacga tgtcgaagca
     4921 gaggtgaatg aagccgcggt cgccccacca tcttccctcg aaaattttac gcggtgtgcg
     4981 cccgttcact ttcaccagct cgatccggct cgacccgagc aaacgcgaga aagcgcccgc
     5041 tctcggtttc gaatgcttca gcagcacgcg acgcacttct gcatttccgc cgggcaggca
     5101 tttgagatca tcgaatacgc cggtcttgtc gtagacaacc gtatcgtaac cgaggatctc
     5161 gccgtagaat ttcaccgagc gttccatgtc cgtaacaccg agcagcgcgc ctgcaggtcc
     5221 gcctgtgagt tgtttccctt tcgcgaacca gtcgtcgccg ggaacgatct cgaaaatatt
     5281 tccccacgga tcgcgcacga aaaagttcgg tgcgccgtcg ggcgatgaag taagtccgtt
     5341 gagaatttcc gcgcctttgc tttccaggaa ttcatacgag cctttcacgt cggacgattt
     5401 catgcgtgtg acgaagtgac cgatgtcgcc gagctgtatc tcgaacggaa ccggaacagg
     5461 cgtgcggctc gtgtactgcc agatctcaaa accgcctccg cctttcatgt tgatggcgag
     5521 gatcgcgtgg cggctgtggc ctttcccgcc ggtgtacgga agcatgaggt tcgcttccgc
     5581 cgcttcttcg aaaacaggaa catccattcc gaaatgccgg cggtaccatt tgaacgcggc
     5641 gtgcacatcg ggaatgccga tgcccacctg ttgtattcct gaaatgatcg gttttgaaat
     5701 cataaaacgg ttgaaaaatt tccagcgaaa ttacaagaat attccggcga attagtgatt
     5761 gacaaatgac gggcaatgcc ccgttccggg caagatcaaa tggtgagcaa tgaatggcgc
     5821 cggatgaaaa gtgccgggcg tggaactaag cgctaacttt accggatgag tttgctccgc
     5881 agcgcatttc agaaaataac agggaatgaa ttcacggacg catcagatga attcctgaac
     5941 tggcttttgg cgtcgtgccc gggcatgcag cacaggggaa atattctctg cttcgggcag
     6001 gcgatacaaa atcttccttc ggaagatccc attgtcgaga tcggcgcgca tgccggactt
     6061 tccgcaaaca tcatttgtta tctcctgaaa aagcacggac ggaagaaccc ggtcatttcc
     6121 atcgatccgt ggatcgtgcg cggctgggat gacgggaaag aaaatgttcc ggaagaatat
     6181 cttttttcgc tgggaagcaa cccgcacgtt aagcgaagtg atttcgctga attcatgatg
     6241 cagtcgtata tccgcaacgt gaaattcttc agcgcggaaa atctcccgtt cgcgttccgc
     6301 atgacgtcgg ataccttctt tgaaaaatgg aattccggtg caattggaaa agacatcatg
     6361 ggcagggaaa tgaattcagg cggaaggatt tcgttcgttt acattgacgg aaaccacgat
     6421 tacgatttcg caaaacgtga ttttgtgaat gcggacaggc atctcgttcc cggcggcttt
     6481 gttcttttcg atgactcgca gagagggagt ccgttcggct gcgcaaaagt ggcggaagaa
     6541 gtgaaagcgg atgagcgata tacgctcgtt gcggaaaacc cgaacctgct cttcaggaaa
     6601 tcgcgcgggt agcaggatat ttaaaataca acgatacaga cagcgtgtga agagcacaaa
     6661 gtaatcgaca cattaatgac cgatatgaca ttcaaaaaca aaaccatctt catcaccggc
     6721 ggaagccgcg ggatcgggaa agcgatcgca cttcgtctcg cgcgcgaggg cgcaaacatc
     6781 gtcatcgctg caaaaacaac ggaaccgcac ccgaagctcg aaggaacgat cttcacggct
     6841 gcggaagaga tcgaaaaagc aggagggaaa tgccttcccg tcaaatgcga tatccgcgag
     6901 gaagaacagg tggtgagcgc ggttgccgac gccgtgaaga atttcggcgg catcgacgtg
     6961 ctcatcaata atgccagcgc catcaatctc tcgccggtgt ccctgcttcc gctgaagcaa
     7021 tacgacctca tgcaggacat caacacgcgc ggcacgtacc tcgtctcccg gcattgcatt
     7081 ccgcatctga aaaaatcttc cggcgcgcat attcttacgt tgtctccgcc gctgtcactc
     7141 gataaaaaat ggttctcgca ttttgcaccg tacaccatca gcaagttcgg catgagcatg
     7201 ctcacgatgg gaatggcgga agaattgcgg aaagacaaca ttgcggtgaa ctcgctctgg
     7261 ccgcgtacga cgatcgccac ggccgcggtg aagaatatcc tcgggggcga tgagctcatg
     7321 aaaaaatcgc gcacgccgga gatcgtggcc gatgcggcat tcttcgtcct ttcggaaaca
     7381 tccgccgtga ccgggaattt tttcatcgac gatgaagtgc tcgcgaaaaa cgggatcaca
     7441 agtctgtctc attatgcagt cgacggagcg caggagctga tgccggatat ttttctgtaa
     7501 agcgacagaa cgtctttttt gtttccgttt tcccgttacc tggttttccg aaaggttaaa
     7561 acgggttgaa cggatgatgg ttttcatgaa tctaagcaaa tactaatccg gctgtccgca
     7621 gagatcagcc tgcgattttc tctttccggc aggaaaaacc tgtcgcccat ggttcgttgt
     7681 tgtgtttccg cgcaaccggg aatgtgccgc agtctaaccg gtttttaatc ggtgcttttg
     7741 gtgaccgctc taattttacc gcagcaacac actctgaaga agtaaataca taccgcgttg
     7801 taaagcaata gggggaaagc ggggaaaggt gaaaagaaaa gtcgccggag aaattccgga
     7861 actttttttc gttttaacgg cccggtaact attatgttaa tatacgcccg cgtaacatgt
     7921 tttagttgtg gggtgaaaat atttgaacca cttttgtgcc ccctccgtgt aaggccctgt
     7981 accagggccg cacgaaaaga gtttataccc ttttcaaaac aaattcatga aaaatcacac
     8041 tctccgattc cggacgctta tgctctccgg aatactcttt tgtaccggaa cagcgttgcg
     8101 tgcgcagctt ccgatgacac gctccgcttt cagcagcgtg tatacgccgg tcagcggaag
     8161 cgcaaccgcg ctcaccttca gttccactga tgacggaacg acgatgattc cgctgccgtt
     8221 cgatttcaac tacctcggga ctacgttcgt gaatacatcg aactacctcg cggtatgcac
     8281 gaacggatgg gcgtcttttt caacgtcgag catcgcaccc tcgaccacgc aggttaacac
     8341 gaacctttac gccgtttcgt ccccgctgaa tactcttgct ccgtggtggg atgatctgaa
     8401 tgttggcgtt gtcggaacaa accccgccgg aggcgcatcc tgggaagtga cgggctctgc
     8461 ccccaaccgt gtcatgacgg tgcagtgggt ggcctcgtct ttttatacga ctaccggcgg
     8521 acagccccgc cagattaatt tccaggtgaa gttgtacgag accacgaacg tcatcgaatt
     8581 ctggtacggc gcagccaccg gggcggtgac caacacgagc gaatcggcga gtatcggcct
     8641 cagtgacgca accggcggca acggccatta cctcgatggt gttacaggtt cctgctacac
     8701 cggtaatgcc gatatgcagt ccgatcgttt tcccacgtac aatctccgct tcactcccgg
     8761 ggcgcccacg ccgctcgcgg ctggcacata taatgtcggc gtcggccaga cgtacatcaa
     8821 cctgaatgaa gctgcagccg atgtaaacca gcgcggcgtc agcggccctg tcgtattcaa
     8881 tctcgtcgat gcacagtatg acgcgaccgc agcaaacggc ggaaactttt tcccgatcct
     8941 catcggaccc atcagcggaa cgagcgcagt caacacggtt actttcacca aatccagcac
     9001 ggcggctgtc atcaggtatg gcggagcttc cggcacaagc ggttcgatcg tcaaccaggc
     9061 gtcgactacc gcccttgcaa gcaacgtcga tccgatcatc ggtctcgtcg gtgcggatta
     9121 tgtgacgctt aataacctcg acatccgcgg caacgtcggc aaccagctcg ccgatcatgg
     9181 cctcggcgtt tataacagtt cggcgacgga tggtgcacaa cacaacacct tcaccaacat
     9241 ttcagttacc atgaaccgcg ccaacaccgg ttcagtgggt attcgccaga gctcggtcac
     9301 gacagcaacg gcttcgaccg gtgccaattc gaacaatatt tacaaggggc tcacgattaa
     9361 aaactgtttc gtgggtatta cgcttagcgg aacgtccggc ttccccgacg acgcctgcga
     9421 gatcggtacg aatgcctgtg gtacgttcaa cgtcatcggt gatccgctta ccgccaatga
     9481 tatcgggaac aacggcggtt ctgtttgggg aatcaagacg acaaacgaga gcaacgtgaa
     9541 aatttacaac aatgtcgtcg tgaatgtgac cggcacgaac gccaaccccg tcgacgccat
     9601 tttcgttgac aatgccggtg tttcggcgac cgtaagcgcc ggcacctgca tggtttacaa
     9661 taataatatc acggcgatta ccagcacatc caccggcacg agcgtggtgc gcggtatccg
     9721 ctgcaacctg aatgccaatg ccggcagtgt gagtgaaatc tacaacaaca cggtttcagg
     9781 tctcaactcg gccagtacct ttacttcttc acgccgcgtg atcggtatca gcgtccagga
     9841 cgcaggcggc ggagcaggtg caacgcataa tgtagacttc aactccgtgc gcattgctcc
     9901 agcagttgca aactgcaaca atacctgctt cgagatcggc actgcgtcgg gaccggtaat
     9961 gaatgtgcgg aataacatct tcgcgaattt taccggcgcc caggccacgg tcaagcatta
    10021 ctgctgggtc agcacttctg ctacttcgaa cggcaacgcc ggttcggttt cgaattacaa
    10081 cgacctctac gtaaacaata cgacgaatgg tttcaccgcc ctcggcaata cgaccgacta
    10141 tgcaacgctg gccaactggc aggcgatcac atccacaccg gatctgaatt cgatcgcagt
    10201 tgatccgcag ttcagttcgg caattgatct gcatacttct gcagccgccc tcgataatgc
    10261 aggcaacaat accggcattc cgtgggtaac ggttgatatt gattgccagg cacgccccca
    10321 accggccgcg acgaatttcg acatcggtgc agatgaaatc acaggctgct ccgggatgcc
    10381 gtcggcagga actgcgggtg gaaacccggc ttccgtttgc ggttccggca ccttcacgat
    10441 ccagactgcc ggcgcttcaa caggtatcgg catcacttat caatggcagg aatcggccac
    10501 accgggcgga ccttatacca actgcacgac cggttccggc atcaatacaa gcacgtatac
    10561 gactgcttct atttcttcaa ccatgtatta tgtctgcctg gtcacctgct caaacagtgg
    10621 cctttcggca acaacgaatg aagtggtggc gactgtaaat gcttctccga cggttaccat
    10681 aacgccgaat cccctcacga tctgcgcagg tggtgcgggc gcctcgctca cagccagcgg
    10741 tgcaaataca tatacctggg cgccttcgac cggtcttagc gctacgacgg gttccactgt
    10801 aacagctaac cctgcttctt ctattaccta tacaattacc ggcacggctg cgaatggttg
    10861 cacttcggct tcgactgtgc ctgtaacggt aaatgaagtt ccaagtctca cttcacctac
    10921 ggcaacgccc gcaacggttt gctccggggc caactcacag ctccttgcga atggcggact
    10981 gaccaacgct tatacgataa gcaacatcac gtatgctcct gtctccacac ccggttctgg
    11041 tgtaacgaca ctggcgaaca acggaaccgc tgtgacggcg ctcaacgcgg gtacgctgga
    11101 cgatggcgac tgggtgaacc aggtcattcc gttcaacttt acctatttcg gaacgatcta
    11161 tacgtctttt gcgctttcca ctaatggttt catctgcctg ggaagcggtg cgccgaacac
    11221 ctatacgtcg tacaacaacg ccatgccgtc tgtcgcaatg gggcgcccgg ctattggtcc
    11281 tgtttacgcc gaccttgatt tccgcactgc cggtacgatc aactacttca caagcggttc
    11341 tgct
//
LOCUS       DASTSF010000597         3048 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1659756,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000597 DASTSF010000000
VERSION     DASTSF010000597.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3048
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1659756"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="VFU15_16860"
     CDS             <1..228
                     /locus_tag="VFU15_16860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C1"
                     /protein_id="HEU4719518.1"
                     /translation="STTDDHGIQIIGWCDKPNGMWFLIKDSGSSARNNPQNPGYYYYH
                     EDYIKLKMMTFTIHKDAVKDVLAKFNTQDSK"
     gene            408..896
                     /locus_tag="VFU15_16865"
     CDS             408..896
                     /locus_tag="VFU15_16865"
                     /inference="COORDINATES: protein motif:HMM:NF039391.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6265 family protein"
                     /protein_id="HEU4719519.1"
                     /translation="MKRILFFLPFLWLLGSCGPSGDVNSISWLVGTWEGHDVKSDFLF
                     HETWSREGTNSLSGFSFISTTDGDTVFKENLRIDHAEGGLYYIVNLPDNKGPVLFRLI
                     KGDDKNAVFENLEHDFPKRISYLRQTANTVEVKLEGIEKGQPKVETLLFERDVDTTLQ
                     KN"
     gene            complement(951..>3048)
                     /locus_tag="VFU15_16870"
     CDS             complement(951..>3048)
                     /locus_tag="VFU15_16870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719520.1"
                     /translation="MKMLTNVHVLSLKNCVNLVSENSFRVIASMPSVTDLVIETKKMD
                     RIPRAFSSFRFMNFISISNTDQSLADGYEMNYAGDANDLFASDSLRLGFGEGSLLLVY
                     SCYDDVTARMHVGVMRDLLQGDVSETGMLSLPGRARAFDRHHPLVKPPVPGPGVCKNV
                     YSTSTLTGAELDYPSGTKISIPANAFVDANGNAVTGNVTIDYREFRDPVDILLSGIPM
                     DYDSAGHNGHFVSAGMFEMNASVDGKEVFLAPGKKVDMKFAVVDTASDFNFYKLDEQE
                     GWQYLDRPGKTETDAPVAAAAKDQQKASAAVLAFWRNIAPVSAKIPGGYDATGFDSRY
                     NDLNYHYESRAPICQGLWNKNSYMQNTFRLHKVYHHKDTTCFSIDRQENARRTYCNPE
                     LSAFSGVRWETKDNLRSKELRNLLRKKSGVNDLRIYQSGDGFVLEFKTSSGFRRINAS
                     AVRMTKKGPKPLSARMTNGMFRHYTKMLDRRRAHMTKSLVRQARRNATSFQQLSRDSV
                     NVWNNCKPVMTKEEAAMDFPGWVKYYRKEFGKIIAQQQVAFSYVQITEGTAAGSIGQA
                     VSLYQALSIASFGIFNCDHVGSIPQPVNVNVELKGNNGEAVAATNIYVIDHSVNSATN
                     FFSFMGKPINVTYNEPSENTLVAISSTGKMYVVDNNALRSGTAIGGSTLYTAKLVSDS
                     VATPEQLREIIYPDGN"
BASE COUNT          686 a          864 c          755 g          743 t
ORIGIN      
        1 agcaccacgg acgatcacgg catccagatc atcggctggt gcgacaagcc caacggcatg
       61 tggttcctca ttaaagattc cggcagcagc gcacgcaaca acccgcagaa ccccggctat
      121 tattattacc acgaagatta catcaagctc aagatgatga ccttcaccat tcacaaagac
      181 gccgtgaaag acgtgctcgc gaaattcaac acgcaggatt ccaaataacg atactcgacc
      241 cttctgtgac aaatgccggt gtttccccgc cggcattttt gttgcccgca acttttcctt
      301 gccggaaact ttttttgtgt cttccggcgg aaaatcggga accggaattg caccggccgc
      361 gcgatgcgta tccttcactc cgttttttga aataacttag cgcaccgatg aaacggatcc
      421 tttttttcct tccttttctc tggctgctgg gttcctgcgg accttccggc gacgtgaatt
      481 ccatttcctg gctcgtggga acctgggaag gccacgacgt caaaagcgat ttccttttcc
      541 atgaaacatg gtcgcgtgaa ggaaccaaca gcctttccgg cttcagcttc atttccacca
      601 ccgacggcga caccgtattc aaggaaaacc tccgcatcga ccacgcggaa ggcggactgt
      661 attacatcgt caatcttccc gacaacaaag gacccgttct tttccgtctc atcaaaggcg
      721 atgataaaaa cgccgtgttc gaaaacctcg agcacgattt cccgaaacgc atttcttacc
      781 tccgccagac ggccaatacc gtcgaagtga agctcgaagg aattgaaaag ggacagccga
      841 aagtcgaaac gctgctgttc gaacgcgatg tggataccac gctgcaaaaa aactgaatga
      901 aaaaagatcc gcgcaggcgg atcttttcgg ggttatgcgg gatgtgccgt ttaatttccg
      961 tccgggtaaa tgatctcccg caattgctcg ggcgtagcga cggaatcaga aaccagctta
     1021 gctgtataaa gcgtgcttcc tcctatcgct gtccccgacc gcaaagcgtt gttgtccacc
     1081 acgtacattt ttcccgtgct gcttatcgcg acgagcgtat tttccgaagg ctcgttgtag
     1141 gtaacgttta tcggtttgcc catgaacgaa aagaagttgg tcgccgaatt cacgctgtga
     1201 tcgatcacat aaatattcgt ggcggcaact gcttcgccat tatttccttt cagctcgaca
     1261 ttgacattca ccggctgcgg tatgctgcca acgtgatcac agttgaatat cccgaatgat
     1321 gcaatgctca gcgcctggta caacgacacc gcctgtccta tgcttcccgc agctgttcct
     1381 tccgtgatct gcacgtatga aaacgcaacc tgttgctgcg cgataatttt tccgaattcc
     1441 tttctatagt atttcaccca tcccggaaaa tccatggcag cttcttcttt cgtcataacc
     1501 ggtttacagt tgttccagac attcactgaa tcccggctga gctgctgaaa ggaagtcgcg
     1561 ttcctgcgcg cctgcctcac aagggacttc gtcatgtgcg ctcttctgcg atcaagcatc
     1621 ttcgtgtaat gacgaaacat tccgttcgtc atccttgcgg agagaggttt cggccctttt
     1681 ttcgtcatgc gcacagcgct ggcgttgatg cgcctgaacc cggacgaagt tttgaattcg
     1741 agcacgaagc cgtctcccga ctggtaaatg cgcaggtcgt tgacaccgct cttcttgcgg
     1801 agcaggttgc gaagctcttt gctgcgcagg ttgtcctttg tttcccatct cacaccgctg
     1861 aatgccgaca gctccgggtt gcagtaggtc ctgcgggcat tctcctgccg gtcgatggaa
     1921 aaacaggtcg tgtccttgtg atgataaact ttgtgaagac ggaatgtgtt ctgcatataa
     1981 ctgttcttat tccataaccc ctggcatatc ggagccctcg attcgtaatg atagttgaga
     2041 tcattatagc gtgaatcaaa accggtcgcg tcatatccgc cggggatttt cgcggacacg
     2101 ggcgcgatgt tccgccagaa agccagcaca gccgcggaag ctttctgctg gtccttcgct
     2161 gcggcggcga cgggcgcatc cgtttccgtt ttgcccggac ggtccaggta ttgccaccct
     2221 tcctgctcat ccagcttgta aaaattgaaa tcgctcgccg tatccaccac cgcaaatttc
     2281 atgtcgactt ttttccccgg cgcgagaaaa acttctttgc cgtccacgct cgcgttcatc
     2341 tcgaacatgc ctgcgctcac gaaatgcccg ttgtgaccgg cgctgtcgta atccatcggg
     2401 atgccgctca gcagaatgtc gaccgggtcg cggaattcgc ggtagtcgat cgtgacattt
     2461 cccgttaccg cgttcccgtt cgcatccacg aaagcgttcg cgggaatgga aatcttcgtg
     2521 cccgacgggt agtcgagctc cgcgcctgta agcgtgctcg tgctgtacac gtttttacaa
     2581 acgccgggcc cgggcaccgg cggtttcacg agcgggtgat gacggtcgaa cgcacgtgca
     2641 cggccgggca atgaaagcat ccccgtttct gaaacgtcac cctgcagcag gtcgcgcatg
     2701 acgcccacgt gcatacgcgc cgttacgtcg tcgtagcagc tgtacacgag cagcagcgaa
     2761 ccttcgccga aaccgagacg caggctgtcg gatgcgaaca gatcattcgc atcaccggca
     2821 taattcatct cgtatccgtc ggcgagcgat tgatcggtat tcgaaatgga aatgaaattc
     2881 ataaagcgga acgaagagaa cgcgcgcggg atcctgtcca tcttttttgt ttcgatgaca
     2941 aggtccgtta cggaaggcat cgatgcgatg acgcggaaag aattttccga aacgaggttc
     3001 acgcaattct tcagcgaaag aacatgcacg ttcgtgagca tcttcatc
//
LOCUS       DASTSF010000598        11376 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_790518,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000598 DASTSF010000000
VERSION     DASTSF010000598.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 11376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11376
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_790518"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(192..4733)
                     /locus_tag="VFU15_16875"
     CDS             complement(192..4733)
                     /locus_tag="VFU15_16875"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEU4719521.1"
                     /translation="MKKGNSLPKPVLKIFLALTCLFLLERNGFSQAGTDCANAVPLGS
                     NSVFLDHQHGSGREFWYSFEPDSSDAWISVINHSLPDSAYHVVKLYSGNCSSLSLIDT
                     VVTALVVKHELTAYSLAPHQSYFIELVSYDTTGGTFDLNLRYFDPAQVQTSCPCHSPY
                     NDPGNTCDLVCNGSFEYPVYQNASGHLDQACPWHNACCSTSDLYTATSALWGVPNNGE
                     GVQTAHTGGNYAGFCPYMYPSCSQFWTTRQDYKEYLIVPLKEPLAAGKCYTFSMYLSC
                     SEQSRFASGDFGVYFSATPLPAQSNNGEYIPLNPQLYFPSIITNTSGWTNLVGTYTAT
                     GGEYQLIIGNFESNVSTPNAQTGFVDFNNISNYPAYSYYYIDDVTLVPCCTAFSVSGP
                     FNSVCTNDTISLTGLSPCQNNSMTWSPANLIIGPNGSSVLAQATPPTTTFYCTMTLTD
                     YGCTKTDSVIVYATQSPYANAGSDTTLCGSRPVVLNGTGTNTSYGWSTLAGQTLCSPC
                     TTVTVTPAVTTSYVYHVYNAHGGCTGYDTMTVFVYPDPHLHIVAPPNAVICDTALHFS
                     LTGLPANYTSVHWTSNAQSAQGSHTPDFSAAWTTNTDAIDGYVTVTVTDADGCTWHDS
                     LVIPHCCCKNEFNVPTGLVATDDNVDSLIAHYPGYITINASGVAVLNSASLCINGVFD
                     VNRDLDLQNVKVAMGPNARINVLQGHTLTLEATGNSFPQQTLIHACYTMWDGIYVYGP
                     DPNTLLDIKDGTVIEDAKNAIVSVAGGNFHIQGGSGSGPVKLNKNYIGIWIKPFSGTH
                     PGYIRKTIIACDNQFQPGGSSGYSSSSNNLIYPYNNNIGYAGILIDSCQSFTVGDTAQ
                     ANYHNVFERMHFGVYARKSNVRVWNNDFRYITSSPVQHKTAPADGVAVYSSGFKNQNY
                     SLIVGVPGTTKAANKIKWCSYGVMATTNVSLSCQYNHLDSCTAIGIYALNNNNSPVLI
                     NQDTLKDCTGTNISCLDNANSTVKITRNVINENVTSSNTGNYGYTGIYVANSVQHHSS
                     VLIQDNTIRKMRNGIWVSRIVHPLILDNSSITIVATQAFTLASPATGIHIEGCASALV
                     RNNHVRYLGTASAANYDKLFGIYIENCFNDTITQDSLINMGSGIFLKGADNPSLLACN
                     KMKNCYYGMNFGYTNSLNNPVPLNDQIHYGTAQTATPTGNHWVGCTTDMRGAIQAPPL
                     GINWWTNAANAPVTINLSGASLTNNNAPIVLTNSDQCTNLLLPPPVKPITMRSYLLHD
                     ICVAPRSYDTLGDQYTYNDDFFAYRALRDSSSWLNLGTSDDTAYQGFYDTTSTSNYTN
                     IAYLADAQDQIAAGNYSQAQADLDYVKYGNNIPENNWKTVMLVYLHSWAIDSMNLTDA
                     QADSLMPIANQGGITGGVAVFDARNMLGLEVHDSSNARIISPIASGDTAQGISIGHAY
                     PNPTTGQVSINANLKEGQTATMNLYDLTGRQITTWKLSGGQSVYTFDASFLPGGTYIY
                     RVESEGQIIEADRLVIIRE"
     gene            complement(5054..7540)
                     /locus_tag="VFU15_16880"
     CDS             complement(5054..7540)
                     /locus_tag="VFU15_16880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     UDP-N-acetylmuramoyl-tripeptide:D-alanyl-D-alanine
                     ligase/alanine racemase"
                     /protein_id="HEU4719522.1"
                     /translation="MIYSIPDIARIVSAEKVILPEDRSVSRLLIDSRNMTHSDGVLFF
                     AIRGERHDAHDFLPDLFSKGVRNFVVSRLPDDCENSYPDSSFILVKDTLAALQTLATH
                     HRKRFTYPVISVTGSNGKTIVKEWLWQLLRDDFSIVRSPKSYNSQVGVPLSVWNMDES
                     NTLGIFEAGISRSGEMEKLESILLPDIALLTNIGTAHDENFTGRSQKTEEKLILLRHA
                     KQVVCCKDDTAVFAMIQQLSEGKQLFTWSRKTKSDLQVARITKEKNETRIQGIFKNRF
                     SEIVIPFTDDASVENAIHCWSMLLLLGTDEETIAARMKQLMPVAMRLELKEGVNGCSV
                     INDSYNSDLGSIAVALDFLNQQQQHARKTVILSDILQSGLDENALYTEVSSLVRSKGV
                     SRFIGIGKAVSHQQEKFAGLDTAFYSNTQEFLANFHSGTFHNETILIKGARPFGFEAI
                     SKALQQKAHETVLEIDLNALVHNLNYFRSRLNPGTQLMAMVKAFSYGSGSFEIANVLQ
                     FHRVDYLAVAYADEGVELRKAGITIPVMVMNPEEQSFDTMIRHGLEPEIFSFRILQLF
                     SEAAKRFRQRHPGQAPVPVHVKLDTGMRRLGFDENELNELIVRLKNSRDLTVRSVFSH
                     LAASDENMHDDFTRKQVKAFITMSDELKKHFSYPILRHILNSAGITRFPDSQFEMVRL
                     GIGLYGIGVNEAEQKNLLPVSTLRTTISQIKNISEGESIGYSRKWIAKSNMRIATVPI
                     GYADGLSRRLSNGGGKMFVHGKACRVVGNVCMDMCMIDVTGISCSEGDEVTVFGKEHP
                     ITDIAKDMGTIPYEVLTNVSRRVKRVYYQE"
     gene            complement(7537..8145)
                     /locus_tag="VFU15_16885"
     CDS             complement(7537..8145)
                     /locus_tag="VFU15_16885"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015753466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEU4719523.1"
                     /translation="MFLENTLHSSKRRGSIEVVCGSMFSGKTEELIRRLRRARIAKLR
                     VEIFKPAIDNRYHEIKVVSHDSNEIHSTPVPGSAAIPELAKDVDVVGIDEAQFFDMGL
                     VNVCNDLADNGVRVIVAGLDMDFLGNPFGPIPALLATAEYVTKVHAICMRCGNLANHS
                     HRISGGKDLVQLGEKDSYEPLCRDCFRDAILHERPPATDFSS"
     gene            8242..8820
                     /locus_tag="VFU15_16890"
     CDS             8242..8820
                     /locus_tag="VFU15_16890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719524.1"
                     /translation="MGVHSENEKRIRQEIADIITDLRPFSDLLKQKDPVPEKEMERLM
                     HDVTMLKRKASVLHYLNANREPEMPPAPQETVKPAAEIVLPPAPVAEVKVREPENPVP
                     PVAEKKEQPKTEKKFPDLKTFIGFNEKIMFLKNLFRNDSAAYEEAIKQLNACASFGEA
                     ESFLSVVKNEYGWKNDSEAVQHFTDTVRRRFS"
     gene            8824..10149
                     /locus_tag="VFU15_16895"
     CDS             8824..10149
                     /locus_tag="VFU15_16895"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEU4719525.1"
                     /translation="MKQILSFFFALAITFSLHAQDRAFAKQVVDTLASPSMHGRGYVN
                     KGDKIAAKYISDEYKSFGLLSFNMAGSYYQEFTFPVNTFPGNMSLTVTTQDGKKVQCV
                     PGQQFLVRPESPSVKGNFPVVLLDSAIVYDDAKWTAFRSMNLKKSFVMVDTSGVRGLR
                     QLQYMKDFAQYPHDVKGLLFPVKPNPIAGSDNCSKLSPWDFSMKQSAISSFEIDYAFR
                     RITAIEVNVTAKFLKNYKSQNVIGYVRGSEEPDSFIVFTAHYDHLGQMGKDTYFPGAN
                     DNASGIAMLLSLARYYSMPEHKPKCSIVFMAFSAEEVGLIGSRYFTMHPLFPMKAIRF
                     LINMDILGTGDEGITVVNATLFDPEFKKLQKMNSDSSYLAVVKPRGKAPISDHFFFTE
                     WGVPSFYIYTLGGIKAYHDTCDRRETLPLTKFESLFALLRDFADWEDRK"
     gene            10243..10932
                     /gene="rsmI"
                     /locus_tag="VFU15_16900"
     CDS             10243..10932
                     /gene="rsmI"
                     /locus_tag="VFU15_16900"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005842662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEU4719526.1"
                     /translation="MPKLHLVPTPVGNLEDITLRALRVLKEADLILAEDTRVTGRLLQ
                     HFDIHTPMQSYHAHNEHRIVTFIAEKIAAGQKVALVSDAGTPGISDPGFLLVRECLAA
                     GIDVECLPGATAFVPALVQSGLPCDSFVFAGFLPQKKGRQTKLRSFEDEKRTMIFYES
                     PFRLVKALGEFRAHFGAERQVCVSRELTKMFEENFRGTVAGAIAHFSAKTVKGEIVIV
                     VAGKNEPGDAE"
     gene            10922..>11376
                     /locus_tag="VFU15_16905"
     CDS             10922..>11376
                     /locus_tag="VFU15_16905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719527.1"
                     /translation="MRSKQDPESDNKTLPWYLRKLWPYFLLAGACFLLYAVTLGYGFS
                     PMDERWLITQEQATLSHFSNLPKLFTTSTLGFYYRPFLNVTFMIDTVTGGGDKMYFHL
                     TNILLHAVCCALLFRFFLVSGIEKHVAFFAALLFAVHPVNVHAVAWIPG"
BASE COUNT         2577 a         2922 c         3137 g         2740 t
ORIGIN      
        1 taattcaact ctgcttgatt atcagggcac tgagaaaaga tttcgaaatc cggattggga
       61 acaagattac cttgtgcgca caaaatcgtg gcaagcaatg aaaaaaacag gaacaaaaat
      121 ttcttcacct aatcaaattt aagcaatccc gccctgtcgc agtaacgaac agggcgggat
      181 caaacgagtt ctcactcacg tattatcact aacctgtccg cttcaatgat ctgtccttcg
      241 ctttctaccc ggtaaatata cgtcccgccg ggaaggaaag atgcgtcgaa tgtatacacg
      301 gattgaccac ctgacaattt ccaggtcgtt atttgtcttc cggtaaggtc atagaggttc
      361 atcgttgcgg tttgaccttc tttgagattg gcattgatgg aaacctgccc ggtcgtcggg
      421 ttcgggtagg catgaccaat ggaaatacct tgtgccgtgt caccgcttgc gatcggtgag
      481 atgatcctcg cgttgctgct gtcatgcact tcaaggccga gcatgttgcg cgcatcgaac
      541 accgcgacgc ctcctgttat tccaccctgg tttgcaatgg gcataagtga atctgcctgt
      601 gcgtcggtca ggttcatgct gtcgatcgcc cacgaatgca ggtagacgag catgacggtt
      661 ttccagttgt tttccggaat gttgttcccg tacttcacgt agtcgagatc cgcttgcgcc
      721 tgtgaataat tcccggcagc gatctggtcc tgggcatcgg cgaggtaagc gatgttcgtg
      781 tagttggatg tgctcgtcgt gtcgtagaaa ccctggtagg cggtgtcgtc cgaagttcca
      841 agattcagcc acgaggaact gtcgcgcagc gcgcggtacg caaaaaagtc gtcgttatat
      901 gtgtactggt ctcccaacgt atcatagctg cgcggcgcga cgcaaatgtc atgcaacagg
      961 tagctgcgca tagttattgg tttaaccggt ggaggtagaa ggagattagt acattgatcc
     1021 gaattggtaa ggacaatagg cgcattatta tttgtcaaac tcgctcctga caaattaatg
     1081 gtaactgggg cattcgccgc attggtccac caattaattc ccaatggtgg tgcttgaata
     1141 gctcctctca tatcagtcgt acatcctacc caatggttac ctgttggcgt tgctgtttgt
     1201 gccgttccat aatggatttg atcattgaga ggaaccggat tgttcaagct attcgtatac
     1261 ccgaaattca tcccataata acaattcttc attttgttgc acgccagcag cgatgggtta
     1321 tccgcgcctt tgaggaaaat gccgctgccc atgttgatca gcgagtcctg cgtgatggtg
     1381 tcgttgaagc agttctcgat atagatcccg aagagcttgt cgtagtttgc ggcggaagct
     1441 gttccgagat acctcacgtg gttgttgcgc accagggcgc tcgcgcaccc ttcgatgtgt
     1501 atgcccgttg ccggtgaggc aagcgtgaac gcctgcgtgg cgacgatcgt gatcgagctg
     1561 ttgtcgagga tgagcgggtg gacaatgcgg ctcacccaga tgccgttcct catcttgcgg
     1621 atggtattgt cctgtatgag cacgctgctg tggtgctgca cggaattcgc cacgtagatg
     1681 cccgtgtagc cgtagttgcc cgtgttggat gaggttacgt tctcgttgat cacgttgcgc
     1741 gtgatcttca ccgtgctgtt cgcattgtcg aggcagctga tgttcgttcc cgtgcagtct
     1801 ttcagcgtgt cctggttgat gagcaccggc gaattattat tgttcagcgc gtagataccg
     1861 atggcggtgc aggagtcgag gtggttgtac tggcagctta aactcacgtt cgtcgtcgcc
     1921 attacgccgt agctgcacca tttgatcttg ttcgcggctt tcgttgtgcc gggcacgcct
     1981 acgatcaatg aataattctg gttcttgaac cccgatgagt aaaccgcgac tccgtcggcc
     2041 ggggcggtct tgtgctgcac cggcgaagaa gtgatgtacc ggaaatcgtt gttccacacg
     2101 cgcacgttcg acttgcgcgc atatacgccg aagtgcatgc gctcgaaaac gttgtggtaa
     2161 ttcgcctgcg cagtatcgcc gaccgtgaag ctctggcagg aatcgataag gatgcccgcg
     2221 tagccgatgt tgttgttgta cgggtaaatg aggttgttcg acgaactgga ataaccggat
     2281 gatccgccgg gctggaactg gttgtcgcag gcgatgatcg ttttgcggat ataacccggg
     2341 tgcgtaccgc tgaacggttt gatccagatg ccgatgtaat ttttattcag cttcaccggg
     2401 cccgaaccac ttccaccctg gatgtggaaa tttccgccgg ccacggaaac gatggcattc
     2461 ttcgcatctt cgatcactgt tccgtcttta atgtcgagca atgtattcgg atcgggtccg
     2521 tacacgtaaa ttccgtccca catcgtatag caggcatgga tcagcgtctg ctgcgggaat
     2581 gaattcccgg tcgcttccag cgtcagcgta tgtccctgca gcacattgat gcgcgcattc
     2641 ggccccatgg ccactttcac attctgcaga tcgagatcgc ggttcacgtc gaacacgccg
     2701 ttgatgcaca ggcttgctga attcagcacc gccacacccg atgcgttgat ggtgatatag
     2761 ccggggtagt gtgcgatcaa tgaatccacg ttgtcgtctg tggcgacgag tcctgtgggt
     2821 acgttgaatt cattcttgca gcagcaatgc gggattacga ggctgtcgtg ccacgtacat
     2881 ccgtccgcgt cggtgacggt caccgtcacg tatccgtcga tggcgtccgt gtttgttgtc
     2941 catgcggcgg aaaaatccgg ggtgtggctt ccctgtgcgg attgtgcatt gctcgtccag
     3001 tgaacggaag tgtaattcgc gggcaatcct gtaagactga aatgcaacgc cgtgtcgcag
     3061 atcaccgcgt tcggcggagc aacgatgtgc aggtgcggat cgggataaac gaaaacagtc
     3121 atcgtatcgt aacccgtgca acctccgtgt gcattgtaga catgatacac gtaggaagtt
     3181 gtcactgccg gcgtcacggt gaccgttgtg catggcgaac aaagcgtttg tcctgcaagc
     3241 gtcgaccagc cgtaagatgt attcgtgccc gttccgttca atactacggg tcgtgaaccg
     3301 cacagtgtag tgtcggatcc cgcgttggcg tagggagatt gtgtcgcata aacaattacg
     3361 ctgtcggttt ttgtacagcc atagtccgta agagtcatgg tgcaataaaa agtggtcgtc
     3421 ggtggagtag cctgggcgag aacactgctg ccgttcggac caatgatcag gtttgcagga
     3481 gaccaggtca tggagttgtt ctggcaggga gataacccgg tcaggctgat cgtatcattc
     3541 gtacatacac tattgaaagg tccgctcaca gaaaaagcgg tgcagcaggg aacaagtgta
     3601 acatcatcta tgtagtaata agagtaggcc ggatagttgc tgatattgtt aaaatcaacg
     3661 aaccctgttt gtgcatttgg cgtgctgacg ttggattcaa aatttccaat gatgagctgg
     3721 tattcgcctc cggtggctgt atacgttccc accaggttgg tccagccgct cgtgtttgta
     3781 ataatggaag gaaagtacag ctgtggatta agtggaatat attccccgtt attgctttga
     3841 gcgggcagag gcgttgctga aaagtacact ccgaaatctc cgctggcaaa acgtgactgt
     3901 tctgaacagg agagatacat cgagaatgta taacacttgc ctgctgcaag aggttctttc
     3961 aacggcacaa tgagatattc cttatagtcc tgccttgtcg tccagaactg gctgcaggaa
     4021 ggatacatat acggacagaa accggcgtag ttgccgcccg tgtgtgcggt ctgtactcct
     4081 tcgccgttat tgggtacgcc ccacaaggct gaggttgccg tgtaaaggtc actggtggaa
     4141 cagcatgcat tgtgccaggg gcaggcctgg tcaagatgcc cggatgcatt ctgataaacc
     4201 gggtactcaa aacttccgtt acagaccagg tcgcatgtat ttcccggatc attataagga
     4261 gaatgacatg gacatgaagt ctgaacctgg gcaggatcga aataccttaa attcagatca
     4321 aaggttcccc cggttgtatc ataggaaacg agctctatga aatagctttg atgaggggca
     4381 agactgtacg cagtaagttc atgtttcaca acaagtgcag tcacgacggt atcgatcaat
     4441 gagagcgaag aacaatttcc ggaataaagt tttacgacat gataggcgct gtccggcaag
     4501 gaatggttga tgaccgagat ccaggcgtcg gatgaatcgg gttcgaaact gtaccagaat
     4561 tcccggccgc tgccatgctg atggtccagg aaaacagaat tgctgcccag cggaacagca
     4621 tttgcgcagt ctgtccccgc ctgggaaaat ccgttccgtt ccagcaggaa aagacaggtg
     4681 agagcaagaa aaattttcaa gaccggtttc gggagagagt ttcctttttt catagggttg
     4741 gattttggat tcagaacaat aaaaaagcta cagtcacagg gatcacgttg ccataaacgg
     4801 tctttcacaa aggaaaaacc gtcgccggaa aaaagcgatc cttcctggtc ctgcagcttt
     4861 cgttccggtc acccggaacg acctttccat cccgcgggaa aggaggattt tcccgcaaaa
     4921 tgcctgtctt acattttgtc taaaatatgt aaatacatga cgcgggtcaa tgcaaacctg
     4981 cgcaatgttt taacaagtcc ggcgacggga attttactcc cggaaagcaa cctcccggga
     5041 atgacgcaac gttttactcc tggtaataca cgcgtttcac tctccgcgaa acgttcgtca
     5101 gcacttcgta aggaatcgta cccatgtctt tcgcgatgtc ggtaattggg tgctcttttc
     5161 cgaatacggt cacttcatcg ccttcgctgc aggaaatgcc ggtcacgtcg atcatgcaca
     5221 tgtccatgca gacgttcccg accacgcggc aggctttgcc gtgcacgaac atttttccgc
     5281 cgccgttgct caggcggcga ctcagcccgt cggcgtaccc gatgggaacc gtggcgatgc
     5341 gcatgttgct tttcgcgatc catttccggc tgtagccgat cgattcccct tcggaaatat
     5401 tcttgatctg cgagattgtc gtgcgcagcg tgctcaccgg caaaagattt ttctgctccg
     5461 cttcattcac gccgatgccg tacagcccga tgccgagccg caccatttcg aactgtgaat
     5521 ccggaaagcg cgtgatgccg gcggaattca ggatgtggcg caggatggga taggaaaaat
     5581 gttttttcaa ttcgtcgctc atcgtgatga acgctttcac ctgctttcgc gtgaagtcat
     5641 catgcatatt ctcgtcgctc gccgcgagat gcgagaatac cgaacgcacg gtaagatccc
     5701 ggctgttctt cagtcgcacg atcagttcat tcagctcgtt ctcatcgaaa ccgaggcggc
     5761 gcatgccggt gtcgagtttc acgtgaacgg gaacgggcgc ctgcccgggg tggcgctggc
     5821 ggaaacgttt cgccgcttcg ctgaacagct gcaggatgcg gaacgaaaaa atttccggtt
     5881 cgagcccgtg gcggatcatc gtgtcgaagc tctgctcttc cggattcatc accatcaccg
     5941 ggatcgtgat gccggccttg cgaagctcca cgccttcgtc agcatacgcg acggcgaggt
     6001 aatccacgcg gtggaactgc agcacgttcg cgatctcgaa gctgccgctg ccgtaggaaa
     6061 acgctttcac catcgccatc agctgcgtgc cgggattcag ccgcgagcgg aaatagttca
     6121 ggttgtgcac gagcgcgttc aggtcgatct cgagaacggt ttcgtgggct ttttgctgca
     6181 acgctttgct gatcgcttcg aagccgaacg ggcgcgcacc tttgatgagt atcgtttcgt
     6241 tatggaacgt tcccgaatgg aaattcgcca ggaactcctg cgtgttggaa tagaatgccg
     6301 tatcgagccc ggcgaatttt tcctgctggt gcgaaaccgc tttgccgatg ccgatgaaac
     6361 gcgaaacgcc tttcgagcgg acaagcgagg acacttccgt atacagcgca ttttcgtcca
     6421 ggccactctg cagtatgtcc gaaaggatca ccgttttccg cgcatgctgc tgctgctggt
     6481 tgagaaaatc gagcgcaacg gcgatggaac cgagatccga gttgtagctg tcgttgatga
     6541 ccgagcagcc gttcacgcct tctttcagct cgagacgcat cgccaccggc atgagctgct
     6601 tcatgcgcgc cgcgatcgtt tcttcgtccg ttcccagcaa taacagcatc gaccagcaat
     6661 ggatcgcatt ctccacggaa gcgtcgtcgg tgaacggaat gacgatctcc gaaaaacggt
     6721 tcttgaaaat tccctgtatg cgcgtttcgt ttttttcttt cgtgatgcgt gcgacctgga
     6781 gatcggattt tgttttacgc gaccaggtaa aaagttgttt tccttcggaa agctgctgga
     6841 tcatcgcaaa gaccgccgtg tcgtccttgc agcagaccac ctgcttcgca tggcgaagga
     6901 gaatgagctt ttcttccgtt ttctggctgc gtcccgtgaa attctcgtcg tgcgccgttc
     6961 cgatgttcgt cagcaacgcg atgtcgggaa gaaggatgga ttccagcttt tccatttccc
     7021 cgctgcgcga aatgccggcc tcgaaaattc cgagcgtgtt gctttcgtcc atgttccaga
     7081 cggaaagcgg gacgcccacc tgcgagttgt agcttttcgg actgcgcacg atgctgaaat
     7141 catcgcgcag caattgccac agccattcct tgacgatcgt tttgccgttg ctgcccgtga
     7201 cggaaatgac cgggtaggtg aaccgtttcc ggtggtgagt ggcgagtgtt tgtaacgcgg
     7261 caagggtgtc tttgacgaga atgaagctgg aatccgggta ggaattttcg cagtcgtcgg
     7321 gcaggcggct gacgacaaag ttccgtacgc ctttgctgaa aagatcagga aggaaatcgt
     7381 gcgcgtcgtg gcgttctccc ctgatcgcga agaacagcac gccgtcgctg tgcgtcatgt
     7441 tgcggctgtc gatgagcagg cggctgacgg aacggtcttc cggaagaata actttctccg
     7501 cgctgacgat ccgggcaata tcgggaatgg aatagatcac gaggagaaat ctgtggccgg
     7561 tggacgttca tggaggatgg cgtcgcggaa acagtcgcgg cacagcggct cgtagctgtc
     7621 cttctcaccg agctgcacaa ggtcttttcc gcccgagatg cggtgcgaat gattggcgag
     7681 atttccgcag cgcatgcaga tcgcgtgcac tttcgtgacg tattccgccg tggcgagcaa
     7741 agcggggatc ggcccgaacg ggttgccgag aaaatccatg tcgagcccgg cgacgatcac
     7801 gcgcacgccg ttatcggcaa gatcgttgca gacattcacc agccccatgt cgaagaactg
     7861 cgcctcatcg atgccgacca cgtccacatc cttcgccagc tcggggatcg ccgcggagcc
     7921 cggcacgggc gtggaatgga tttcattgga atcgtgcgac acgactttga tctcgtggta
     7981 ccggttgtcg atcgccggct tgaagatctc gacgcggagc ttcgcgatgc gcgcgcggcg
     8041 caggcggcgg atgagctcct ccgtcttgcc ggaaaacatg gagccgcaca ccacttcgat
     8101 ggagccgcgg cgtttgctgg aatgaagcgt attctcgaga aacatgatcg ttcggaaaaa
     8161 agggccccgc cgggaaaatt gtacctttga aaccgctggg gaaaccctgc aaatctaacc
     8221 aaaagcggag ttcctgttac catgggtgtt cattctgaaa acgaaaaaag gatccggcaa
     8281 gagatcgcgg atattatcac cgacctccgg ccattttccg acctgctgaa gcaaaaggat
     8341 cctgttcctg aaaaggaaat ggaacggctc atgcatgatg tcacgatgct gaaacggaaa
     8401 gcgtccgtgc tgcattacct gaacgcgaac agggagccgg aaatgccgcc cgctccgcag
     8461 gaaacggtaa agcccgctgc agagatcgtt cttcccccgg cgcctgtggc ggaagtaaaa
     8521 gtccgcgaac cggaaaaccc cgttccgccc gttgctgaaa aaaaggaaca gccgaagacg
     8581 gaaaaaaaat tccccgacct gaaaaccttc atcggcttca acgaaaagat catgttcctg
     8641 aaaaatcttt tccggaatga cagtgccgct tatgaagaag cgataaagca gcttaacgcg
     8701 tgcgcttcat tcggggaagc ggagtcgttc ctttccgtcg tgaagaacga atacggatgg
     8761 aaaaacgaca gtgaagccgt gcagcatttc accgacaccg tcaggcgacg tttttcctga
     8821 gctatgaaac agatactttc tttctttttc gcccttgcaa tcaccttttc gcttcatgcg
     8881 caggatcgtg catttgcaaa acaagtcgtc gatacgctcg cttcgccttc catgcacggt
     8941 cgcgggtacg tgaacaaagg tgacaagatc gccgcaaaat atatttccga cgagtataaa
     9001 tcgttcggac ttctttcctt caacatggcg ggaagctatt accaggaatt cacgttcccg
     9061 gtgaatacgt tcccggggaa catgagtctc acggttacga cgcaggacgg aaaaaaagtg
     9121 cagtgtgtgc ccggccagca attccttgtc cggcccgaaa gcccttcggt gaaaggaaat
     9181 ttcccggtcg tgctgctgga ttctgcaatc gtttacgacg atgcaaaatg gaccgcgttc
     9241 aggagcatga acctgaaaaa atctttcgtg atggtggata caagcggtgt gcgcggattg
     9301 cggcagctgc aatacatgaa agattttgcg caatatccgc acgacgtaaa aggactcctg
     9361 ttcccggtga agccgaatcc cattgccggt tccgataatt gctcgaagct gagcccgtgg
     9421 gatttttcga tgaagcaatc ggctatttct tccttcgaga tcgattacgc tttccgccgc
     9481 attacggcga tcgaggtgaa cgtgacggcg aaattcctga agaactacaa atcgcagaac
     9541 gtgatcgggt atgtgagagg ttccgaggaa cccgattcgt tcatcgtgtt caccgcgcat
     9601 tacgatcacc tcgggcaaat gggaaaggat acttatttcc ccggcgccaa cgataacgcc
     9661 agcggaatag ccatgctcct cagtcttgcg cgttattatt cgatgccgga gcataaaccg
     9721 aaatgctcca tcgtattcat ggcgttcagc gcagaggaag tcgggctcat cggctcgcgg
     9781 tatttcacga tgcacccgct gttcccgatg aaagcgatca ggttcctcat caacatggac
     9841 attctcggaa cgggcgatga aggcatcacc gttgtgaatg ccacgctgtt cgatcctgag
     9901 ttcaaaaagc tgcagaagat gaacagcgac agcagctatc tcgccgttgt aaaaccgaga
     9961 ggcaaagcgc cgatcagcga tcatttcttt ttcaccgaat ggggcgtgcc ttccttttac
    10021 atttacacgc tcggcggaat aaaagcctac cacgatacct gcgacaggag agaaacgctg
    10081 ccgctcacga aattcgaatc gctttttgcg ctgctgcggg attttgcgga ctgggaagac
    10141 aggaagtgac gaatgacaaa ccgtgaaccg taaatgatga attaatggga gaccttcttt
    10201 ttaagagttg tcattcctga tccatccttt atcctttcaa acatgccgaa gctgcatctc
    10261 gttcccacac cggtcggaaa tctcgaagac atcacgcttc gcgcgctgcg cgtcctgaaa
    10321 gaagcggacc tcatcctggc ggaagacacg cgtgtcacgg gaagattgct ccagcatttc
    10381 gatattcaca cgcccatgca atcgtatcac gcgcacaacg agcacaggat cgtgacgttc
    10441 atcgcggaaa aaattgcggc gggacaaaaa gtagcgctcg tgagcgatgc cggaacgccg
    10501 ggcatttccg atccgggatt tcttctcgtc cgcgaatgcc ttgccgccgg gatcgacgtg
    10561 gaatgcctgc cgggcgctac ggcgttcgtt cctgcgctcg tgcagtcggg gcttccctgc
    10621 gattcgttcg tgttcgccgg ctttcttccg cagaaaaaag gaaggcagac gaagctgcgt
    10681 tctttcgaag atgaaaagcg gacgatgatc ttttacgaat ctccgttccg ccttgtgaaa
    10741 gcgctcgggg aattccgggc gcatttcgga gcggaaaggc aggtgtgcgt gtcgcgcgag
    10801 ctgacgaaaa tgttcgagga aaatttccgc ggcacggttg ccggagccat tgcgcatttt
    10861 tcagccaaaa cggtaaaagg cgagatcgtg atcgtcgttg ccggaaagaa cgagcccgga
    10921 gatgcggagt aaacaggacc cggaaagcga taataaaaca ctgccgtggt acctgcggaa
    10981 actgtggccg tatttcctcc tggccggggc ctgctttctc ctttacgcag tgacgctggg
    11041 ttacggtttt tccccgatgg acgagcggtg gctgatcaca caggagcagg cgacgctttc
    11101 gcatttctca aatcttccga agctgttcac cacatccacg ctgggttttt attaccgtcc
    11161 ttttctcaat gtaacgttca tgatcgatac ggttacgggc ggcggtgata aaatgtattt
    11221 ccatctcacc aacatcctgc ttcatgcggt ctgctgcgcg ctgctgttcc gttttttcct
    11281 tgtctccgga attgaaaagc acgttgcttt tttcgccgcg ctgctcttcg ccgtgcaccc
    11341 cgtgaacgtg catgccgtgg cgtggatacc gggaag
//
LOCUS       DASTSF010000599         2371 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1849691,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000599 DASTSF010000000
VERSION     DASTSF010000599.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2371
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1849691"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..496
                     /locus_tag="VFU15_16910"
     CDS             <1..496
                     /locus_tag="VFU15_16910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010855691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycerol acyltransferase"
                     /protein_id="HEU4719528.1"
                     /translation="NLTMLNELFVPVNKHGTQHAIEKIEQVYRSDHAVLIFPAGLVSR
                     KQSGKIMDLEWKKSFISKSVQYHRNIIPTYVDGKNSAFFYNLALWRKRLGIKANIEMF
                     YLADEMFRQKNKTITIIFDKPVSPHVFDRSKSHRDWAQEMKKHVYAIGRGEPGPFTLN
                     HPNE"
     gene            509..1441
                     /locus_tag="VFU15_16915"
     CDS             509..1441
                     /locus_tag="VFU15_16915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002999419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU4719529.1"
                     /translation="MQPVIPAVPRTSLLRELNEKTFIREANNGDNQIYIINAHNAPET
                     LKEIGRLREVTFRAAGGGTGKDCDLDEFDTNEKCYDQLIVWNPDDQEIVGGYRFIRCK
                     KAKDAHGHFHLATTELFRFSEKFTNEYLPRTIELGRSFVQPSYQPSINNRKGLFSLDN
                     LWDGLGALVVDNPDIDYFFGKVTTYRHFNVQARDYILHFMDHYFPDPEKLVTPIHPVR
                     ITTDISAFEKELGRLPYKEGHKLLNQHVRALGENIPPLVNSYMNLSATMRNFGCAVNS
                     EFGDVDETGIMVTIGDIYPTKKERHIGTYHKKNP"
     gene            1561..2166
                     /gene="yihA"
                     /locus_tag="VFU15_16920"
     CDS             1561..2166
                     /gene="yihA"
                     /locus_tag="VFU15_16920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HEU4719530.1"
                     /translation="MKISSAKFLVSSSKLSQCPKADKPEYAFIGRSNVGKSSLINMLC
                     GIHNLARISAKPGKTQLINHFIINDSWYLVDLPGYGFAKVSKELRSGFDRMISSYIMG
                     RTNLHYLFVLVDARLEPQQIDLDFINKLGENGVPFCIVFTKSDKLSATKLKKSTDLFL
                     GTLKESWDELPPYFITSSETKKGRDEILSLIAETNRMFEAS"
     gene            complement(2170..>2371)
                     /locus_tag="VFU15_16925"
     CDS             complement(2170..>2371)
                     /locus_tag="VFU15_16925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719531.1"
                     /translation="ISPDDRMNLLYGFNWMRAGLLLPGKWSSSDREYFAQYQCWQGNY
                     YICAGISYDDFYRRSKHWRITA"
BASE COUNT          607 a          704 c          551 g          509 t
ORIGIN      
        1 caaccttacc atgctgaacg agcttttcgt cccggtgaac aagcacggaa cgcagcacgc
       61 catcgaaaag atcgagcagg tgtacaggtc ggatcacgcc gtgcttattt ttcccgcagg
      121 actcgtttcc cggaaacagt cgggaaagat catggacctc gaatggaaaa aaagcttcat
      181 ttcgaaatcc gttcagtacc accggaacat cattcccacg tacgtcgacg ggaaaaattc
      241 cgcgttcttc tacaacctcg cgctgtggag aaaacgtctc ggcatcaaag ccaacatcga
      301 gatgttctac ctcgccgatg aaatgttccg gcagaagaat aaaacaatta ccattatctt
      361 tgataagccc gtttcaccgc atgttttcga tcgctcaaag tcgcaccgcg actgggcgca
      421 ggaaatgaaa aagcatgtgt atgcgatcgg ccggggcgaa ccgggtcctt ttacactgaa
      481 ccatccgaac gaataaggaa ttccgtttat gcagcctgtt attcctgccg taccgcgcac
      541 ttccctgctc cgtgagctga acgaaaaaac attcatacgc gaagccaaca acggggacaa
      601 ccagatatac atcatcaatg cgcacaacgc gcccgaaaca ctgaaagaga tcggccgcct
      661 gcgcgaggtc acgttccgcg cggccggcgg cggtaccggg aaagactgcg acctcgacga
      721 gttcgacacg aacgaaaaat gctacgatca gctcattgtc tggaacccgg acgaccagga
      781 gatcgtcggc ggctaccgct tcatccgctg caaaaaagcg aaggatgcgc acgggcattt
      841 tcatctcgcc accacggagc ttttccgttt cagtgaaaaa ttcacgaacg aataccttcc
      901 ccgcaccatc gagctcggac gctccttcgt gcagccgtct taccagcctt ccatcaacaa
      961 ccgcaaagga cttttttcgc tcgacaatct ctgggacggg ctcggtgcgc tcgtcgtcga
     1021 caatcccgat atagattact ttttcgggaa agtcaccacc tatcgtcatt tcaacgtgca
     1081 ggcacgcgac tacatcctgc atttcatgga tcattatttt cccgatccgg aaaaactcgt
     1141 cacgccgatt catcctgtca gaatcaccac agatatttcc gcgttcgaaa aagagctcgg
     1201 ccggcttcct tacaaagagg gacacaagct cctcaaccag cacgtgcgtg cgctcggcga
     1261 gaacattccg ccgctcgtga attcctacat gaacctcagc gcaaccatgc gcaatttcgg
     1321 ctgcgccgtc aattcggaat tcggcgacgt ggatgaaacc ggcatcatgg tcaccatcgg
     1381 cgacatctac ccgaccaaaa aagaacggca catcggcacg taccacaaaa aaaatccgtg
     1441 atcgttcagg agcgcgtcac ctgtgctttt cattttccct gctcccgctt tccgttccac
     1501 tgcaatcggg gctaaggaat tttctcctgc gtttcaaaac acctgcgtta tctttgcgcc
     1561 gtgaagatct cctctgcaaa atttctcgtc agcagctcga agctttcgca atgtcccaaa
     1621 gcggacaagc ccgaatatgc gttcatcgga agatcgaacg tgggaaaatc ttcgctcatc
     1681 aacatgctct gcgggatcca caacctcgca cgcatttccg cgaagccggg aaaaacgcag
     1741 ctcatcaatc atttcatcat caacgacagc tggtacctcg tcgatcttcc cggctatggt
     1801 ttcgcaaaag tttcgaaaga gctgcgcagc gggttcgacc gcatgatcag ttcctacatc
     1861 atgggacgca cgaacctgca ttatcttttc gtgcttgtcg atgcgcgcct cgaaccgcag
     1921 cagatcgatc tcgacttcat caacaaactc ggcgagaacg gcgttccgtt ctgtatcgtc
     1981 ttcaccaagt cggacaagct ttccgcgaca aagctgaaga aaagcacaga cctgtttctc
     2041 ggcacgctca aagaaagctg ggacgaactg ccgccgtatt tcatcacatc ttccgaaacg
     2101 aagaaaggcc gcgacgagat cctctcgctc attgctgaaa cgaaccgcat gttcgaagct
     2161 tcgtgaacgt cacgctgtga tccgccagtg cttcgaccgg cggtaaaaat catcgtacga
     2221 tatccccgcg cagatatagt aattcccctg ccagcactgg tactgcgcaa agtattcgcg
     2281 gtcggaagaa gaccatttcc ccggcagcag cagcccggcg cgcatccagt tgaagccgta
     2341 gagcaggttc atcctgtcat cgggactgat g
//
LOCUS       DASTSF010000600        10510 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_138686,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000600 DASTSF010000000
VERSION     DASTSF010000600.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 10510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10510
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138686"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..758)
                     /locus_tag="VFU15_16930"
     CDS             complement(<1..758)
                     /locus_tag="VFU15_16930"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU4719532.1"
                     /translation="MRKILGKVKNKILGRKPVDQRSARISQYIAGGRIPWSTGYYEYK
                     WDFISHSLDDKTLLGVFQKHEPLPAGYGSGLDDRAVEYPWIFSRIRSGKSYFLDAGST
                     FNFPAIVNLPVVREKDLTILTFFPEPYAFNKQRVSYVYADLREMPLRDNWFDEIVCQS
                     TLEHVGMDNSMYGYKDAPPDIGKGKDFSYLKVIHELVRVLKPGGQLLMTFPFGKFEFH
                     GFFQQFDNEMLDSMLDEFGKTGRTETEFFLYTKDG"
     gene            complement(755..1396)
                     /locus_tag="VFU15_16935"
     CDS             complement(755..1396)
                     /locus_tag="VFU15_16935"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEU4719533.1"
                     /translation="MGLLKKINNRIRKYLLSQVITPEEFVQLAREKEIEQYRMRMVLG
                     DGSKLYDNARVFNLQGQREQIVAGQNTHIRGDLLVFAYGGRIQIGDNCYVGDGSRIWS
                     GEDVRIGNNVLIAHNVNIIDSNSHEMNYLERAEGFLGLMRDGYPKEKGSILTAPVVIG
                     DYAWISFGSIILRGVTIGKGGVVAAGSVVTKDVPPFAVVAGNPAVVVKQMEIQ"
     gene            complement(1407..2207)
                     /locus_tag="VFU15_16940"
     CDS             complement(1407..2207)
                     /locus_tag="VFU15_16940"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HEU4719534.1"
                     /translation="MGLFQRIFGTTASEKKTSPLSCSFREDDVFLVSFPKSGNTWVRF
                     LIGNYVTRGGMDFSNGHLFMPDIHFNPEQMNELKTRPRFLKSHNPYTPDYSRVIYLVR
                     EGRDTAVSYFHFLKKMKSISPETTFSEYLENFFFAGRSPYGQWNAHVFSWLRDHRVPH
                     LLLVKYEDLLVNAREELRKMVVFSGLAVDEKLLDLAVERSSFASMKEDEKRNSDSLRK
                     LGHDMPAEDNSIVRSGKSNSWREVFSEAENEKFIRLYGDALDYLGYKR"
     gene            complement(2321..3142)
                     /locus_tag="VFU15_16945"
     CDS             complement(2321..3142)
                     /locus_tag="VFU15_16945"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HEU4719535.1"
                     /translation="MISLFGRKNKKEAFPLDNIFHCCAHKTGSQWVRAFMSDPLIRQK
                     TGLEVHTYMKSMPEGVDSRKVDERFFDQPFPLKKIVSPLYLSRQGYESIPKPESFASF
                     YIYRNPRDILISYYFSVRETHAPNEKLSKARSELQQLGAEEGLIYAIDSMNEQGYFRA
                     LSSWFEKPVTADEGSGKMKKFRFEDLTDFSTGLIAELFLHCSIRLSKNELASIESAYS
                     FASMQKKDGRKEKGHYRSGVHGEWKKYFTERVTEKFTAVTGDLYSRMGYEESDKR"
     gene            complement(3186..4475)
                     /locus_tag="VFU15_16950"
     CDS             complement(3186..4475)
                     /locus_tag="VFU15_16950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008464401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU4719536.1"
                     /translation="MSNPVISVEHIWKQYRVGLVGAGTLSSDLKRWMHRIRGKEDPFS
                     KIADTNDRTVKGNSDYVWALQDVSFQVSEGEVLGIMGKNGAGKSTLLKILSRVTAPTK
                     GEIKIRGRIASLLEVGTGFHPDLTGRENVFLNGTILGMKRAEIRAKFDEIVAFSGVER
                     YIDTPVKRYSSGMYVRLAFAVAAHLDPEILVVDEVLAVGDAEFQKKCLGKMKDVSGKG
                     RTILFVSHNLAAVRNICTRAVLIEKGTVRFEGDTNEAIDLYMSDNTGSASFDGMVMWG
                     DAEMPGNKFYKVRSVRLLNEKGEVSSVFLTNKSVSVEVRYEVMSPITGLRVSVTFIDR
                     QGTIAFVSTNQISETEEKQPGQYVSTCRVPANLLNGGGYEVSVRIGKPWAQDLAVALD
                     NILAFDTTLMGNNGSVARSAWPGVVAPALEWITEKTA"
     gene            complement(4481..5350)
                     /locus_tag="VFU15_16955"
     CDS             complement(4481..5350)
                     /locus_tag="VFU15_16955"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU4719537.1"
                     /translation="MSSRDQGAELKWDIIVRPKSKWYNLDLKGVWQYRDLVALFVKRD
                     FLALYKQTVFGPLWMLLQPLLMTLTFSIIFGRVANISTGDAPRLLFYMVAYIPWTYFS
                     DCLNRTATTFTSGAGIFGKVYFPRMVTPISMIISSFLRFLVQLVLIVGLYFFFVYRGT
                     VVRPNAHLAYFPLLILLLAGYGLAAGLVISALTSKYRDLTFIIPLVLQLLMYGSSVVI
                     GFAQFGPELQKYLRWNPLLWIFEAIRYSLLGSGTGLWSWHALAYATGIMLVLLVAGIF
                     SFSSRERTFIDTV"
     gene            complement(5516..7897)
                     /locus_tag="VFU15_16960"
     CDS             complement(5516..7897)
                     /locus_tag="VFU15_16960"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HEU4719538.1"
                     /translation="MKKLLPYVKKNWWIVLLFAGISLLAGDLYDYKLDKVYDVSTQLL
                     LKSNEDYSAGSLITDNRFYGNTTKTFVDNSNEMRILQSHDLVDQVLDRLDFDVSYYLV
                     GRLRTTEVYAGVPFSVKISSLNPELYEQQIRFRILTPEQYEMTFQVGGQSKTVKGFFD
                     KELLNPYMKLMVSQKRNVLNPHGQADNLANYIIQPHRKEDLAQKFLSETKIENPEYTN
                     VLKVSCFDVLPEKAMKFLDTLSEVYIENSINSKLDINKNTVYFIDRQLDEVANILNNI
                     EDTLQDFREKNSVIDLDRQSQDYFDKYSSYLDQKKNLEIQIGTLDDLEKYVIENKDPS
                     FLPPAAYSLQGDEFQISTLQWLYDRENKLKEELNIARPGNFAVQNLKNQIDSTKRDLL
                     IYISNTRTALGEKMDRVDTTISFYDGLLNSLPEKERGMLNISRKQKVNEDIYVFLLQR
                     RANTVISRASIVPDTKIIERPRVNGIVSPDDKKIIISFLGGGVALAFLLVFIRIFYFH
                     RIETYDELKLYTQLPIIGDVVISKIPTDLSIAVEFEPKSPLAESFRTIRTNLQYMATG
                     SGPHVIVITSNSPGEGKTFCSLNLAAVLAKSGKKTVLVELDLHKPRIQTGLGIKSEKG
                     ISTIASGRHTIAECVVQTKIEGLDAILSGPLPPNPSELVSSKQIDLLLEYCKANYEFV
                     IIDTPPVGLISDAMVLMTKANVLLFVMNTKYAFRHALQNATEIAMLNQDIHFGFILNG
                     VKRKKSRYYYSHYGYGYGYGSYGGGYGGYGGYGQGSYGGYGGYGTQNKPKKKK"
     gene            8104..>10510
                     /locus_tag="VFU15_16965"
     CDS             8104..>10510
                     /locus_tag="VFU15_16965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719539.1"
                     /translation="MKKAILLLLLTTAFSVKSHSAVFTSIATGNYHAASTWSFTGSDA
                     DGIPDLDDNVTITSGKTVTLTGTENCKTLTINPGGIINLNLNTMRVYGDFTNNGTSTG
                     SGSWQFRAAGTYSGNNLPNNGTVYFYAPYSIAAGVVMSKPGYMVVSASVILSNFGNVT
                     LTAGSIQLLSGAKWINKSGSTVSVSTNVISSGTIDASAASNNFIYTGGGTTTVQATNA
                     TYYNLILSSGAGANTKALNGNLTVLNNLNINSNATLNWNNKNITLGGNWVNNANTACT
                     NMATITFNGSGTQTITRSSQEFFNNVTLSGSGTVSIATNVRADGTTTLSSGTLDPGAF
                     FYKQAGISWLNNGGAINTAATGSVLFNATVNQTIGGTTSTTFGNFGINNSGGSVILAL
                     NQTVTGVTTLLSGILDVSSFSFNQSGSTFSFSGGTLNTAATGKVVFTGSSAQTITSAA
                     TPSFGNVEINSSSTVTPATDVIVLGTTTMTSGTLDPSTFTWHQQGTSWVGNGGSVNAA
                     ASGEVVFDGAVNQTIGGSTGTTFGNLEFNNAGHSITLSVNSGGAATTLLTAGTFDVST
                     FSFSQSGDTWSATGGTLNTGASGKVIFTGAGTQSITAPSTFAFGNLEINAGGTVQLQI
                     DITVAGTFTLTAGIFDVTTPANYGVKLLGDVVSNGATFQPRTGTVTLAGAGTQTISGS
                     SQFQVYDLLDINSISISVTGLVSISDILTVSAGSFKTNGSGTVVLTATGPTTYARIAP
                     LGAGAILAGTGWIVQSYIDGPATAYWQYLSTPIKNNQLSDWDNDTRFYMSGVGGNDGN
                     ACCP"
BASE COUNT         2553 a         2849 c         2489 g         2619 t
ORIGIN      
        1 ccgtctttcg tgtacaggaa aaattcggtc tccgtcctgc cggttttgcc gaactcatcc
       61 agcatgctgt ccagcatttc attgtcgaac tgctgaaaaa atccgtggaa ctcgaacttt
      121 ccgaacggga acgtcatgag cagctgtccg cccggtttaa gaacgcgcac cagttcgtga
      181 atgactttca ggtagctgaa atcttttcct ttcccgatat ccggaggagc atctttgtag
      241 ccgtacatgg aattgtccat tcccacatgc tcaagcgtcg actggcagac gatttcatcg
      301 aaccaattgt cgcgcagcgg catttccctc aggtctgcgt atacatacga aacacgctgc
      361 ttgttgaatg cataaggctc ggggaagaac gtgaggatcg tcaggtcttt ttcgcgcacc
      421 acgggaaggt tgacgatcgc cggaaaatta aatgtcgatc ccgcatcaag aaaatacgac
      481 ttcccgctcc tgatccttga aaaaatccag ggatattcca ccgccctgtc atcaagtccc
      541 gaaccataac ccgccgggag cggttcatgt ttttgaaaaa cgccgagcag cgttttatca
      601 tccagcgaat gggaaataaa atcccatttg tattcataat acccggtcga ccagggaatg
      661 cgtccgccgg caatatactg gctgatcctg gcgctccgct gatctacggg tttacgaccg
      721 aggattttat tcttgacctt gcccagaatt tttctcattg gatttccatt tgtttcacca
      781 cgacggcggg atttcccgcc acaactgcaa acgggggaac gtcttttgtc accacgcttc
      841 ctgctgccac aactccgcct ttcccaatgg tcactcccct gagaatgatg gacccgaagc
      901 tgatccaggc gtaatcaccg atcaccaccg gcgccgtgag tatgcttcct ttttctttcg
      961 ggtaaccatc tctcatgagg ccgagaaagc cttccgcccg ctcaagataa ttcatctcat
     1021 gcgaattgga gtcgatgata tttacgttgt gcgcgataag cacgttgttc ccgatacgga
     1081 cgtcctcacc cgaccagatc ctgcttccgt ctcccacata gcaattgtcg ccgatctgta
     1141 tccggccgcc ataggcgaac acgaggagat ctccgcgtat atgcgtgttt tgcccggcaa
     1201 cgatctgctc cctttgtccc tgcaggttaa atacacgcgc attatcgtag agcttcgaac
     1261 cgtccccgag caccatccgc atcctgtact gttcaatctc tttttcacgg gcgagttgaa
     1321 caaattcttc cggcgtgatg acctgcgaaa gcaggtactt ccggatgcgg ttgtttattt
     1381 ttttcagaag ccccattgtg cagcgcttat cttttatagc cgaggtaatc cagggcatcc
     1441 ccgtacagcc ggataaattt ttcattttcc gcctccgaga aaacttcccg ccagctgttg
     1501 ctttttccgc tcctcacgat tgaattatcc tccgccggca tatcatgtcc gagcttgcga
     1561 agactgtcgc tgttcctttt ttcatcctcc ttcatcgatg caaaggaaga tcgttccact
     1621 gcaagatcga ggagcttttc atccacggca agccctgaaa aaacgaccat tttccgcagc
     1681 tcttccctgg cgttcaccag caggtcctcg tattttacaa gcagcaggtg cggcacgcga
     1741 tgatcgcgga gccatgaaaa aacgtgggca ttccattgac cgtaaggaga tcttccggcg
     1801 aaaaagaaat tctcaagata ctcgctgaac gtcgtctccg gggaaatgct cttcatcttt
     1861 ttcaggaaat ggaaatagct gacggctgtg tcgcggcctt cacgtacgag atagatcacc
     1921 cgcgaataat caggtgtgta cgggttgtgg cttttaagaa acctcgggcg ggtcttcaac
     1981 tcgttcatct gctccgggtt gaaatgaata tcgggcatga agaggtggcc attgctgaaa
     2041 tccattccgc cacgggtgac gtaattaccg atgaggaaac gcacccatgt gtttcccgat
     2101 ttgggaaatg aaacaagaaa tacatcgtcc tcccggaaag aacaggacaa cggcgatgtc
     2161 tttttttcgg aggcagttgt gccgaaaata cgttgaaata gtcccataga aggcactgca
     2221 attaagttct gtcccggctg aaaggccgct tcaatgcgat ttgtcaatca tttgcttccg
     2281 gaatggatac gcctgccggg ccgttcaagc ccgcggatgt tcatcgttta tccgattcct
     2341 cgtagcccat gcgggaatac agatcaccgg taacagcggt gaatttttcc gtaacccgct
     2401 ccgtaaaata ttttttccat tcgccgtgaa cacccgaacg gtagtggcct ttctcttttc
     2461 ttccgtcttt tttctgcatc gaagcaaatg aatacgccga ctcgatggaa gccagctcgt
     2521 ttttcgaaag ccggatgctg cagtgaagga aaagttccgc aataagcccc gtgctgaaat
     2581 cggtaaggtc ttcaaacctg aattttttca ttttaccgga tccttcatct gcggtaaccg
     2641 gcttttcaaa ccagctgctc aaggccctga aataaccctg ttcgttcatc gaatctatgg
     2701 cgtagatcag cccctcttcc gcacccagct gttgcagctc gctcctcgct tttgaaagtt
     2761 tttcattcgg cgcatgtgtt tcgcgaacgg aaaaataata ggagataagg atgtctctcg
     2821 ggtttctgta aatgtagaac gacgcgaacg attccggctt gggtatcgat tcatatccct
     2881 gccggctgag gtaaagcggg ctcacgattt ttttcagcgg gaacggctga tcgaagaaac
     2941 gttcatccac tttccggctg tcgactccct ccggcatgga cttcatgtac gtatgaacct
     3001 caagccccgt tttttgcctg atcagcggat cgctcatgaa agcccgcacc cactgacttc
     3061 ccgttttatg cgcgcaacaa tggaaaatat tatccagcgg gaacgcttcc tttttatttt
     3121 ttcttccgaa gaggctgatc atggtcgaat ttaaatattt ttttgcggcg ctgccgtcgt
     3181 cattcctatg cggtcttttc agtaatccat tcgagcgccg gcgcaacaac acccggccat
     3241 gcggaacggg ccaccgatcc gttatttccc atcagcgttg tatcgaatgc caggatatta
     3301 tcgagcgcca ccgcgagatc ctgcgcccac ggtttcccga tcctcaccga tacttcataa
     3361 ccgcctccgt tcaacaggtt ggccggcacc ctgcaggttg agacgtactg acccggctgc
     3421 ttctcttccg tttcactgat ctggttcgtg gaaacaaatg cgatcgttcc ctggcggtca
     3481 ataaacgtga cggaaacacg gagaccggtt atcgggctca tgacctcgta cctgacctcg
     3541 acgctgacgg atttattcgt aagaaaaacg gaagacacct cgcctttttc attcagcagt
     3601 ctcaccgatc ttactttgta aaatttattg ccgggcatct ccgcatctcc ccacatgacc
     3661 atgccgtcaa agctcgccga gcctgtattg tcagacatat acagatcgat cgcttcgttt
     3721 gtatcgcctt caaaacgcac ggtacctttt tcgatcaata cagcccgcgt gcagatgttc
     3781 cgtaccgccg caaggttgtg gctgacgaaa agtatggtgc gtccttttcc gctcacatct
     3841 ttcattttcc cgagacattt tttctggaac tcggcatcac ccaccgcgag aacttcgtcc
     3901 accacgagta tttccggatc gaggtgcgca gcgacggcaa acgcaagacg gacatacatc
     3961 ccgctgctgt atcgcttgac cggcgtgtcg atgtaccgct ccaccccgct gaacgccaca
     4021 atctcatcga acttcgcgcg gatttcagcc cgcttcatcc cgaggatcgt tccgttgagg
     4081 aaaacatttt ccctcccggt aagatcggga tgaaaaccgg tgccgacctc aagcagggaa
     4141 gcgatccttc cccggatttt tatttcacct ttcgtaggcg cagttacacg cgaaagaatt
     4201 ttcaaaagcg tcgacttgcc ggctccgttc ttacccatta ttccgagcac ttctccttca
     4261 cttacctgga acgaaacatc ctggagcgcc cacacataat ccgaattgcc cttcaccgta
     4321 cggtcattcg tatccgctat tttggaaaac ggatcttcct tgcccctgat gcggtgcatc
     4381 caccgtttca ggtcggagct cagcgtaccc gcaccaacca gcccgacgcg gtactgtttc
     4441 catatatgct cgacggaaat aacgggattg ctcatagctt ctacaccgta tcaataaatg
     4501 ttctttcccg gctgctgaac gaaaatattc cggcaacaag cagcaccagc atgattccgg
     4561 tggcataggc cagcgcatgc cacgaccaca ggcctgttcc gcttcccagc agcgaataac
     4621 gtatcgcttc gaatatccag agaagcgggt tccagcggag gtacttctga agctcaggac
     4681 cgaattgcgc aaaaccgatg accactgaag agccgtacat cagcagctga agtaccagcg
     4741 ggataataaa agtcaggtcg cggtatttgc tcgtcagcgc gctgatgaca agcccggcgg
     4801 ccaacccgta acccgccagc agcaggatca gtaacgggaa ataagcgaga tgcgcattgg
     4861 gcctgacaac agtacctctg tacacgaaaa aaaagtacag cccgacaatg agcaccagct
     4921 gcacgaggaa gcgcagaaag ctggatatga tcatggagat cggcgtcacc atgcgtggga
     4981 agtacacttt cccgaaaata ccggccccgg aagtgaaggt cgtcgcagtt ctgttcaggc
     5041 agtcggaaaa ataggtccac ggtatataag ccaccatgta aaaaagcagc ctcggggcgt
     5101 cacctgtcga gatattcgca acccgtccga aaatgatcga aaaggtaagc gtcatcagca
     5161 atggctggag aagcatccac aggggaccga aaacggtctg cttataaaga gcgagaaaat
     5221 cccttttaac gaataatgca acaagatcac ggtattgcca aactcccttt aaatccagat
     5281 tgtaccactt tgacttgggc ctgactatga tatcccattt caattcagcg ccctgatctc
     5341 tgctcgacat aaacctccgg gtcttttgac tggtgaaata acattgctac caaagataat
     5401 caaaataaaa gcctttgcgt ccttacacaa ccgctgctgc aagccgttcc gtgtagtctg
     5461 cgaaatcctt tgaaggcaaa ccgttcagga atgaaaaact cagcttccgg cacggtcact
     5521 ttttcttctt cggcttgttc tgcgttccgt aaccaccgta gccgccgtaa gaaccctggc
     5581 cataaccacc gtagccgccg taaccgccac cgtacgagcc atagccataa ccgtaaccgt
     5641 agtggctgta ataataacgc gatttcttgc gcttgactcc gttcaggata aaaccaaaat
     5701 ggatgtcctg gttgagcatt gcgatctcag tcgcattctg gagcgcgtga cggaacgcat
     5761 atttggtatt catcacgaaa agcagcacgt tggctttcgt catcagcacc atcgcgtctg
     5821 atatcagtcc gaccgggggt gtgtcgataa tcacaaactc ataattggcc ttgcaatatt
     5881 cgagcagcag atcgatctgc ttcgagctga ccagctccga cggatttggc ggcagcggtc
     5941 ctgaaaggat cgcatccagt ccttcgatct ttgtctgcac gacgcattcc gctatcgtat
     6001 gccttccgga agctatcgtg gatattcctt tttcagattt tattccaagg cccgtctgta
     6061 tcctcggctt gtgaagatcc agctccacga gtacggtctt tttgcctgat ttggcaagca
     6121 ccgcggcaag gttcagcgag cagaatgttt tgccttcgcc cgggctgttc gacgtgatca
     6181 cgatgacgtg aggtccgctt cccgtagcca tatactgaag atttgtgcgg atcgtgcgga
     6241 acgactcggc cagcggagac ttcggttcga attcgacagc gatggaaaga tcagttggta
     6301 ttttggagat gacgacatcg ccgataatgg gaagctgcgt gtaaagtttg agttcatcgt
     6361 aagtctcgat gcggtggaaa tagaatatcc gtataaaaac aagaaggaat gccagggcaa
     6421 caccgccgcc gagaaaggag atgattattt tcttgtcgtc cgggcttaca ataccgttca
     6481 cccgcggcct ttcgatgatc ttggtgtccg gaactatgct ggctcttgat atgaccgtat
     6541 tcgcgcggcg ctgcagcagg aaaacgtaaa tgtcctcgtt tactttttgc ttgcggctga
     6601 tattgagcat tcccctttct ttttccggga gactgttcag cagcccgtca taaaaagaga
     6661 tcgtcgtatc cacccggtcc attttttctc cgagtgcggt tcttgtattg gaaatgtaaa
     6721 tgagaagatc acgcttggtc gagtcgatct ggtttttcag gttctgtacg gcaaagtttc
     6781 ccggtctcgc gatattgagt tcctccttga gcttgttttc cctgtcgtac agccactgaa
     6841 gcgtgctgat ctggaattca tctccctgca gagaatacgc ggcaggagga agaaatgacg
     6901 gatccttgtt ttcgatcaca tatttttcaa ggtcatccag cgtcccgatc tgtatctcca
     6961 ggtttttttt ctgatcgagg tagcttgaat atttatcgaa ataatcctgc gactggcgat
     7021 cgaggtcaat aacggaattc ttctcccgga aatcctggag cgtgtcctcg atgttattca
     7081 ggatattggc tacttcatcc agctgcctgt cgatgaaata gactgtattc ttgttaatgt
     7141 ccagttttga gttgatggaa ttttcaatgt aaacctcgga aagggtatcg agaaatttca
     7201 tcgccttttc gggcaataca tcgaagcacg atactttcag cacattcgtg tattcggggt
     7261 tctcgatttt tgtctccgac aaaaatttct gcgcaagatc ctctttccgg tgcggctgga
     7321 taatataatt ggcaagatta tccgcctgcc cgtgcgggtt cagcacattc cgcttctgtg
     7381 acaccatcag cttcatgtag ggattcagaa gctctttgtc gaaaaatcct ttcacggtct
     7441 tgctctgtcc gccaacctgg aaagtcatct cgtattgctc cggggtaagt atcctgaacc
     7501 tgatctgctg ttcataaagc tcggggttga ggctggagat cttcacggaa aaaggaacgc
     7561 ctgcgtacac ctcggtggtg cgcaatcttc ccaccaggta ataggaaacg tcaaaatcaa
     7621 gacggtcgag gacctggtcg accaggtcat gcgactgcag gatgcgcatt tcattggaat
     7681 tatcgacaaa ggtcttcgtc gtatttccgt aaaaacggtt atcggtaatg aggctgcctg
     7741 ccgagtagtc ctcgttggat ttcagcagca gctgcgtgga aacatcatat actttgtcga
     7801 gcttatagtc atacagatcg cccgcaagaa gggatatacc ggcaaaaagc aggactatcc
     7861 accagttctt ttttacatac ggcaaaagct ttttcaactc cccgattccg aacgccccct
     7921 gttgattctg ctgtttcata agcgcgccta aattaccaat tatttggcag gagaaattgc
     7981 ttgggaacca atggataaaa tgtggcgtaa aaagaggtaa atgccttgat atcgtagggc
     8041 aaaacgacac ccgctatagg aggacccgtt cgatttgatt atctttgtga aaaccggctt
     8101 gctatgaaaa aagctatact tcttcttttg ctgacgacag ctttttccgt gaaatcccat
     8161 tccgcggttt ttacatcaat tgcaacaggc aattatcatg ctgcctccac gtggagcttt
     8221 acaggttccg atgcggacgg cattcccgac ctggatgata atgttacgat cacctcagga
     8281 aagacagtca cgcttaccgg aaccgagaac tgcaaaacac tcacaataaa tccgggggga
     8341 ataatcaacc tcaacctgaa caccatgagg gtgtatgggg attttaccaa taacgggacc
     8401 tcaaccggct ccggctcctg gcagttccgg gctgccggaa cttattccgg gaataacctt
     8461 cccaataacg gtactgttta tttctatgcc ccttattcaa ttgctgccgg cgtggtgatg
     8521 agcaaaccgg gctatatggt tgtttccgcc agtgtgattc tctccaactt cggcaacgtt
     8581 acgcttaccg cggggagcat ccagttgctg agcggcgcca aatggataaa caaatccgga
     8641 tccacagttt ccgtatcgac aaatgtcatc tcttccggaa cgatcgatgc ttccgccgct
     8701 tccaataatt tcatttacac aggaggtgga acaacaacgg tccaggcaac caacgccacc
     8761 tattataacc tcattttgtc atcgggagcg ggagcgaata cgaaagcgct caatggcaac
     8821 ctcactgtat tgaacaacct taacatcaac agcaacgcaa cgctcaactg gaataacaag
     8881 aacatcacgc ttggcggaaa ctgggtcaac aatgcaaata ccgcctgcac caacatggcc
     8941 acgatcactt tcaacggctc gggcacgcaa accatcacgc gcagctccca ggaatttttc
     9001 aataacgtta ccctttccgg cagcggcacg gtttccatcg caaccaacgt gagagctgac
     9061 ggcaccacta ctctttccag cggcacactg gatcccggag catttttcta caagcaggcc
     9121 ggcatatcgt ggctgaataa cggcggcgct ataaacaccg cagcaaccgg cagcgtcctg
     9181 ttcaatgcta cggtgaacca gaccatcggc ggaacgacga gcaccacatt tggaaacttc
     9241 ggaataaaca acagcggggg aagtgtaatt ctcgctctta accagacggt gacgggagtt
     9301 accactcttc ttagcggaat actggatgtt tcttcgttca gtttcaacca gtcgggcagc
     9361 acattcagct tttcgggcgg cacactgaat accgcagcca ccggcaaggt tgtttttacc
     9421 ggctcttctg ctcaaaccat tacatcggct gcaactccgt ctttcggaaa tgtggaaata
     9481 aacagctcaa gcacggtaac gccggccacg gatgtcattg tgcttggaac gacaacgatg
     9541 acaagcggca cattggatcc ttccacgttt acgtggcacc agcagggaac atcctgggtg
     9601 ggcaacgggg gatctgttaa cgcagcagcc agcggagaag ttgtatttga cggagccgtc
     9661 aaccaaacca tcgggggaag cacaggaacg actttcggaa atctcgagtt caataacgca
     9721 gggcattcca ttactctttc tgtcaactcc gggggagcag ccaccaccct gctcacggcc
     9781 ggcacgttcg acgtgtccac cttcagcttc agccagtcgg gcgacacctg gagcgccacg
     9841 ggcggaacac tcaatacagg tgccagcgga aaagtgattt tcacgggagc aggaacgcag
     9901 tccattaccg ctccatccac cttcgctttc ggaaacctcg agatcaacgc aggaggaacc
     9961 gtgcagctgc agatcgatat tacagttgcc ggaactttca cgctgaccgc cggaattttc
    10021 gatgtgacca cgcctgccaa ttacggagtg aagcttttgg gagatgttgt ttcaaacggc
    10081 gcaacgttcc agccgagaac aggtaccgtt acacttgccg gtgcaggtac gcagaccatc
    10141 agcgggtctt cccagttcca ggtgtatgat cttctcgata ttaactcgat cagcatttcg
    10201 gtaacaggtc ttgtttcgat ctcggatatt ctcaccgtaa gcgcaggcag cttcaagaca
    10261 aacggatccg gaacagttgt cctcaccgcg accggcccga ccacttacgc acgcattgct
    10321 cccctcggag cgggcgccat actcgccgga acaggatgga tcgtgcaatc atacattgac
    10381 ggtcccgcca cggcatactg gcaatatctt tcgacaccga tcaagaacaa ccagctcagc
    10441 gactgggata atgatacccg cttttacatg agcggcgtcg gaggtaacga cgggaatgcc
    10501 tgctgtccca
//
LOCUS       DASTSF010000601         6633 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_6969,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000601 DASTSF010000000
VERSION     DASTSF010000601.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 6633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6633
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6969"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..156
                     /locus_tag="VFU15_16970"
     CDS             <1..156
                     /locus_tag="VFU15_16970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013306152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030527 - structural constituent of
                     chromatin [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEU4719540.1"
                     /translation="GFGSFIVKRRAEKTGRNISKNTTVIIPAHNVPAFKPAKTFVEKV
                     KKNVKAE"
     gene            167..1018
                     /locus_tag="VFU15_16975"
     CDS             167..1018
                     /locus_tag="VFU15_16975"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU4719541.1"
                     /translation="MTAFTYKHIIVFGLAASCIVLLNLVPTFPSEGKNAALVKQQQQQ
                     GPGVDELVKTARSKMAPDLAGKVTTIENAIATERDFSKRGQMYDSLITLLGRNKEYLV
                     AAWYAEQKAVKNNGSGSDWEQAGERYRTSASFEQNDAVLPSVYAAAMRCFSKALELEP
                     SNVDAKIGLGICYVEGTSDPMTGIGMLLDVERADSTNVNVQLALADFAVRSQQYDKAI
                     ARYSKALRLKPEYYGLHLNLAELYQQMGDTASAISHLEEYVKLENDPLVKNDVENAIR
                     QLRGNKQ"
     gene            1359..2909
                     /locus_tag="VFU15_16980"
     CDS             1359..2909
                     /locus_tag="VFU15_16980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010601126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HEU4719542.1"
                     /translation="MSNELVIDASGPEVVIALLSDRRLVELNREKTNTQFAVGDIYLG
                     RVRKVMPGLNAAFVDVGYEKDAFLHYFDLGPQVLSLNKFVRMTITGKQKHSALAYFKP
                     EPDINKDGKISSVLQPDQQVLIQVAKEPISTKGPRITSELSIAGRYIVLIPFADKVSV
                     SQKIKSNEERERLKRLIQSIKPKNFGVIVRTVAEGKGVADLDADMNDLVSKWNTCFQE
                     LTKSKPPARVLGELNRTSAILRDMLNSSFSSITVNDPRMFDEIREYLQSIAPDKEKIL
                     KLHKGPGHIFEQNGVDRQIKALFGKTVTMKSGAYLIIEHTEALHVIDVNSGNRSKLHS
                     DQESNALDVNLEAAVEVARQLRLRDMGGIIVVDFIDMHSPLNRKALFNKLRDEMKNDR
                     AKHTILPPSKFGLVQITRERVRPETSVDTAEKCPTCGGSGEVQASILLMDQIENNLRY
                     LLKEQNEPSVTICVHPYIEAYLTKGIFSQRWKWSRKFGKKIHVKPVSAYQFLEYHFLN
                     KNQDEIKV"
     gene            2978..3211
                     /locus_tag="VFU15_16985"
     CDS             2978..3211
                     /locus_tag="VFU15_16985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719543.1"
                     /translation="MAKHVSTKQFFTPFRALLTGGRADFSDAEGQLIFGAQLSHPGEV
                     LLPQDNPADGIYMVRFTTSSGGIYSGKIIIQKN"
     gene            complement(3239..3994)
                     /locus_tag="VFU15_16990"
     CDS             complement(3239..3994)
                     /locus_tag="VFU15_16990"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEU4719544.1"
                     /translation="MSDTASILTTQNVAINYNIASAGERTLARLLDLVIMAVYLVAMF
                     LISNQVFSGGDWEMRETFQLPVSILITVPVLTYTLWCEPVFRGRTFGKIILGLKVVKT
                     DGTPAGVGAAAFRWMTRLLEGELALFTFLALPVAIISGKGQRIGDMVAGTIVINTKTK
                     VSLRSTIISHINPDYRVVFPQVSVLSDRDMNIIRDVMQQAYDTGNYHLLEYLGHKVKT
                     VMGVFPPPQQLPTAQFLNIVLADYAHYNFQGKA"
     gene            4137..5435
                     /locus_tag="VFU15_16995"
     CDS             4137..5435
                     /locus_tag="VFU15_16995"
                     /inference="COORDINATES: protein motif:HMM:NF014052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HEU4719545.1"
                     /translation="MKEITFLKQNHEKWQQFETLLDSRQQSDPDALADLFIRITDDLS
                     WARTFYPGSNTEKYLNTLAARVHQEIYKNKKERRSRILTFWSDELPRIMAAERMNLLI
                     VFAIFLVSVWIGVISAKHDEGFVRIIMGDNYVDKTIKHIKEGDPLGVYKSDNQFAMFF
                     GIALNNARVAILCVLAGLLGWIGVSVLQFQNGVMLGAFLEFLAKNKQLHEAMLTVWIH
                     GVIEISCIIVAGAAGIRFASGFWFPGAWPRGVAFRRSARDGLKIAFGLIPFFFLAAFF
                     EGFITRYTEMPDVLRLLIIYGSVIFIAWYFILYPQFVKKLAGGEFFTLDRVPLAGRFF
                     RGTKYEGKTFNHRIAFLLMIGTIMIVLPVFAMVMQVVVHKAADPAAYLFYSISITGGG
                     FCIRMALGSVGLREENDLTIDRKTRQKTAEYERNAGTVKI"
     gene            5404..6369
                     /locus_tag="VFU15_17000"
     CDS             5404..6369
                     /locus_tag="VFU15_17000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719546.1"
                     /translation="MSATQAPLRFERVRNFGRVFSETRSFLKENFTTFFLSLLFLAGP
                     VVIITCALEMYYEIELAGPNDFPSLDRIGSYLAMTTFYREGRWLINGLVIAIVVSHFV
                     KVYREKGPGKFDVGDVTRSLFRNLGSSLTGFIIIFLGVAIITAVIGFLVYGLAELSIG
                     AAVLVLLSCGIAYFLIRFPFWYLVYSVFFANTSGDRGIFGSIGFAGKFFSGNWWSTWV
                     IFFCMWLLLYLVGVAISLPAEVLTGIAKLSSITSGNDDVDWKLIQTIAGTLGEFARTM
                     VNSVFCIAIALHFHSLKEKVDGEGTKKLVESIGTKQEDEQVDLTY"
     gene            6380..>6633
                     /locus_tag="VFU15_17005"
     CDS             6380..>6633
                     /locus_tag="VFU15_17005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719547.1"
                     /translation="MRLFPGIAVLLFFLFAVQQLSAGNGEVPVKPVYDSSGVVKKQPS
                     VSTQDKYYGDKYWRYEKEEKEAGSQEPGVFDRMLKSFIKNL"
BASE COUNT         1613 a         1799 c         1785 g         1436 t
ORIGIN      
        1 gggttcggca gcttcatcgt taaacgtcgc gctgaaaaga ccgggcgcaa catttcgaaa
       61 aacacgaccg tcatcattcc cgcacataac gttccggctt tcaagcctgc caagaccttc
      121 gttgaaaagg tcaagaagaa cgtaaaagct gagtaacagg atcgccatga ccgctttcac
      181 ctacaaacac atcattgtgt tcggactggc ggcatcctgt atcgttcttt taaatcttgt
      241 accgactttt ccctccgaag gaaaaaatgc tgcgctggta aagcagcagc aacagcaggg
      301 acccggggtt gacgagctgg tgaaaaccgc acgttcgaaa atggcgccgg acctggcggg
      361 aaaagtcacc acgatcgaaa atgcgatcgc caccgaacgt gatttttcaa agcgagggca
      421 gatgtacgat tcgctgatca cgctgctggg ccggaacaaa gagtatcttg ttgcggcctg
      481 gtatgcagag cagaaagcgg tgaagaacaa cggttcgggc agcgactggg agcaggcggg
      541 tgaacgttac cgcacatcgg cctccttcga gcagaatgac gccgttcttc cgtctgtcta
      601 tgctgcggca atgcgctgct tcagcaaggc gctcgagctg gaaccttcga atgtggatgc
      661 gaagatcgga ctcggcatct gctacgtgga aggcacgagc gacccgatga caggcatcgg
      721 gatgctgctc gacgtggagc gcgccgattc gaccaacgtc aacgtacagc tggcgctggc
      781 tgatttcgca gtccgttcgc agcaatacga caaagcgatc gcccggtact caaaagcatt
      841 gcggctgaag cctgaatatt acgggcttca cctgaatctc gccgaattat accagcagat
      901 gggcgacacg gcttcagcga tctctcacct ggaagagtat gtgaagctgg aaaacgatcc
      961 actggtaaaa aatgacgtgg agaatgccat acggcagctc cgcgggaaca aacaatagta
     1021 gtaactaaaa aatctttcaa cctatgccaa gcggcaaaaa acgtaaacgt cataagatgg
     1081 cgactcacaa gcgtaagaaa cgtcttcgta agaatcgtca caagaagaag aaccgctagt
     1141 aagccgttta cggtttccac gcattccatc attgctttcc gccggggaac attctccggc
     1201 ggaaagtgtg aaccgcatca gagtacgaac tgattacaca ggccgaccct cctgctcccg
     1261 gaaccgtttc cccgcttcat tcttcacagt ttatcggatc tcccatgacg cattctgtca
     1321 atggaagatc tatgctgcct gaaaaaaacc aacgtaacgt gtcaaacgaa ttagttatcg
     1381 acgcatcagg tcccgaggta gtcatcgcac tgctgagcga caggcggctg gtcgagctta
     1441 accgcgagaa gaccaacacg cagttcgctg taggcgatat atacctgggc cgcgtgcgca
     1501 aagtcatgcc cggacttaac gcggcattcg ttgacgtcgg ctacgaaaaa gacgcttttc
     1561 ttcattattt cgacctgggt ccgcaggtgc tttcgctgaa caagttcgtc cgcatgacga
     1621 tcacggggaa gcagaagcat tccgcgctgg cgtatttcaa gccggagccg gacatcaaca
     1681 aggacgggaa gatctcttct gttctgcagc ctgatcagca ggtgctcata caggtggcga
     1741 aagagccgat ctcgacgaaa ggtccgagga tcacttcgga gctttccatt gcaggacgtt
     1801 atattgttct gatcccgttc gcggataaag tttccgtttc ccagaagatc aagagcaatg
     1861 aagaacggga acgcctcaag cggctgatcc aaagcatcaa accgaagaac ttcggcgtca
     1921 tcgtccgtac ggtggccgaa ggaaaaggtg tagcagacct cgacgcagac atgaacgatc
     1981 tcgtaagcaa atggaacacg tgtttccagg agctgacgaa atcaaaaccg ccggctcgcg
     2041 tgctcggcga gctcaaccgc acatccgcca tcctgcgcga catgctgaac agctcgttca
     2101 gctccatcac ggtgaacgac ccgcgcatgt tcgacgagat cagggaatac ctgcagtcga
     2161 tcgcgcccga caaagagaaa atactcaagc ttcacaaagg gccgggacat attttcgagc
     2221 agaacggcgt cgaccgccag atcaaagcgc tgttcggaaa aacggtgacg atgaaaagcg
     2281 gtgcgtacct catcatcgag catacggaag cgctgcacgt gatcgacgtc aacagcggca
     2341 accgctcgaa gctgcacagc gaccaggaaa gcaacgcact cgacgtgaac ctcgaggccg
     2401 cggtggaagt agcgcgccag ctccggctgc gcgacatggg cggtatcatc gtcgtcgact
     2461 tcatcgacat gcattccccg ctcaaccgca aagcgctttt caacaagctg cgcgacgaga
     2521 tgaagaacga ccgcgcgaag cacacgatcc tcccgccgag caaattcggc ctcgtgcaga
     2581 tcacgcgcga acgcgtacgg ccggaaacca gcgtcgacac cgcggaaaaa tgtccgacct
     2641 gcggcggaag cggcgaagtg caggccagca tcctgctcat ggaccagatc gagaacaacc
     2701 tgcgttatct tttaaaagaa cagaacgaac cctccgtcac gatatgcgtt cacccgtata
     2761 ttgaagcgta tctcaccaaa ggcatttttt cacagcgctg gaaatggagc cggaaattcg
     2821 gcaagaaaat ccacgtcaaa cccgtttcag cctaccagtt tctcgaatat catttcctga
     2881 acaagaacca ggacgagata aaagtctgac agtaaaaatc ccggaagctt tttccgggat
     2941 tttttatttc ctcactgcga aaatctgcca tcccggattg gccaaacacg tttccacgaa
     3001 gcagtttttt accccgttcc gggcgctgct taccggcggg cgcgccgact tttccgatgc
     3061 ggaaggacag ctgatcttcg gcgcacagct ttcacatccc ggggaagtgc tgcttccgca
     3121 ggataatccg gcagacggga tttacatggt gcgcttcacc acatcatcgg gcggcattta
     3181 ttccgggaaa atcattatac agaaaaactg acccggggaa tttccgaagc cggaaaactt
     3241 acgctttccc ttggaaattg taatgcgcgt aatcggccag gacgatattg aggaactgcg
     3301 cggtcggcaa ttgctgcggg ggaggaaaaa cccccatcac cgttttcact ttgtgcccga
     3361 ggtattcgag gagatgataa tttcccgtat cgtaggcctg ctgcatgaca tcgcggatga
     3421 tattcatatc gcgatcggaa aggacggaca cctgcggaaa aaccacgcgg taatccgggt
     3481 tgatgtgcga gatgatagtg ctgcgcaggg aaactttcgt tttggtgttg atcacgatcg
     3541 ttcccgcgac catgtcgccg atgcgctgtc ccttgccgct gatgatcgcc acgggcaatg
     3601 cgaggaatgt aaagagggcg agctcgcctt cgagcaggcg cgtcatccag cggaaagccg
     3661 cagcaccgac tccggcaggc gttccgtcgg tcttcaccac tttcagcccg agaatgatct
     3721 tgccgaatgt ccttccgcgg aaaaccggtt cgcaccagag cgtgtatgtg agaacgggca
     3781 cggtgataag aatggaaacc ggcagctgga acgtttcgcg catttcccaa tccccgcccg
     3841 aaaagacctg gttcgaaatg aggaacatgg caacgaggta caccgccatg atgacaaggt
     3901 cgagaagacg tgcgagcgta cgttcacctg ccgaggcgat attgtaattg atcgcaacgt
     3961 tctgcgtggt gagtatgctt gcggtatccg acaagaaaaa agtgtttggg aacgcccggg
     4021 tttacgatca acgaacttac gaattattaa gaaaaatgca ccgggcagac tgacgctgta
     4081 atttgtaaat tcgtggaggt tccggtcaac cgtcccgcac aacctcgccg gcatttatga
     4141 aagaaatcac ttttctgaag cagaaccatg aaaagtggca gcagttcgaa acgctcctcg
     4201 actcaaggca gcagagcgat cctgatgcgc tggcggatct tttcatccgc atcaccgacg
     4261 atctttcctg ggcgcgcact ttttatcccg gctcgaacac cgagaaatac cttaacacgc
     4321 tcgctgcgcg cgttcaccag gaaatctaca agaacaaaaa agaacggcgc agccgtatcc
     4381 tcaccttctg gtcggacgag ctgccgcgca tcatggcggc ggaacgcatg aacctgctga
     4441 tcgtattcgc gatctttctc gtttccgtct ggatcggcgt tatttccgca aagcacgacg
     4501 aaggatttgt gcgcatcatc atgggcgata attacgtcga caagacgatc aagcacatca
     4561 aagagggcga cccgctcggc gtttacaaaa gcgacaacca gttcgccatg ttcttcggca
     4621 tcgcactgaa taatgcacgc gtggcgatac tgtgcgtgct ggccgggctg cttggctgga
     4681 tcggcgtgtc cgtactgcag ttccagaacg gcgtgatgct gggcgctttc cttgaattcc
     4741 tcgcaaagaa caagcagctc cacgaagcca tgctgaccgt atggatccac ggcgtgatcg
     4801 agatcagctg cattatcgtg gcaggtgcgg cgggcatacg tttcgcgagc ggcttctggt
     4861 ttcccggcgc gtggccgaga ggcgttgcgt tcaggcgcag cgcgcgcgac gggctgaaga
     4921 tcgcgttcgg gctcatcccg ttctttttcc tcgccgcatt tttcgaaggg ttcatcacgc
     4981 gttacacaga gatgccggac gtactccggc tgctgatcat ttatgggtcc gtcattttta
     5041 tcgcgtggta ttttattctt tatccgcagt tcgtgaaaaa gctcgcgggc ggcgaattct
     5101 tcacgctcga ccgggtgccg ctggccgggc ggtttttccg cggaacgaaa tacgaaggaa
     5161 aaacattcaa ccaccgcatc gcatttctcc tgatgatcgg aacgatcatg atcgtgcttc
     5221 ccgtcttcgc gatggtcatg caggtggtcg tgcacaaagc agcggacccg gcggcctatc
     5281 tcttctacag catttccata acgggaggag gcttctgcat ccggatggcg ctcggttccg
     5341 tcggactgcg cgaagaaaac gacctcacga tcgaccggaa aacccggcaa aaaacagccg
     5401 aatatgagcg caacgcaggc accgttaaga tttgagcgcg tccggaattt cggccgggta
     5461 ttcagtgaaa cgcgcagctt cctgaaggaa aatttcacga cgttctttct ttccctgctc
     5521 ttcctcgccg gccctgtggt gatcatcacc tgtgcgctgg aaatgtatta cgagatcgag
     5581 ctcgccgggc cgaacgattt tccatcgctg gaccgtatcg ggtcgtacct ggcgatgacc
     5641 actttttacc gcgaaggaag atggctgatc aacggcctgg tgatcgccat cgtggtgagc
     5701 cacttcgtta aagtgtaccg ggaaaagggc ccgggtaaat tcgacgtggg cgatgtgacg
     5761 cgctcccttt tccgcaatct tggctcttct ctcaccggat tcattattat ttttctcggc
     5821 gtcgcgataa taaccgcggt tattggtttc ctggtgtatg ggctcgcgga gctctccatc
     5881 ggcgctgccg tgctggtgct tctctcgtgc ggcattgcgt attttctcat acgtttcccg
     5941 ttctggtacc tcgtgtattc ggtctttttc gcgaatacat ccggtgaccg cggcattttc
     6001 ggctccatcg gttttgccgg gaaatttttt tcgggcaact ggtggtcgac gtgggtgatc
     6061 ttcttctgca tgtggctgct gctttacctc gtcggcgtgg cgatctcgct tcccgcagaa
     6121 gtgctcacgg gcatcgcaaa actttcctcg atcacgtcgg gcaacgacga tgtcgactgg
     6181 aagctgatac aaaccatcgc cggcacactc ggtgaattcg cacgcaccat ggtgaacagc
     6241 gttttctgca tcgccattgc gctgcatttc cacagcctga aggaaaaggt tgacggcgaa
     6301 ggcacgaaaa agctggtgga atccatcggc acaaaacagg aagatgaaca ggtcgatctc
     6361 acgtactgaa gcccggcgca tgcggctgtt cccgggaatc gcggttctcc ttttctttct
     6421 tttcgcggtt cagcaacttt ctgcaggcaa cggtgaggtc ccggtgaaac ctgtttacga
     6481 ttcttccggt gtcgtaaaaa aacaaccttc cgtttcgaca caggataaat attacggcga
     6541 taaatactgg cgctacgaaa aagaagaaaa ggaagccggc tcacaggaac ccggtgtatt
     6601 cgaccggatg ctgaaaagtt ttataaaaaa cct
//
LOCUS       DASTSF010000602         7759 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_344817,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000602 DASTSF010000000
VERSION     DASTSF010000602.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 7759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7759
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_344817"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(102..1067)
                     /locus_tag="VFU15_17010"
     CDS             complement(102..1067)
                     /locus_tag="VFU15_17010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719548.1"
                     /translation="MKATLLFSFLLFSLLSPAQKISYDTSRSDNGTIYWTGIHYRYTD
                     GGGYDRWTFYYENGTKRLEEITDTSFRVHKLVNAWLQDGTQTLKNGNGIYVYSGTDST
                     IYRVKDSLYNGYAVSYYHFYEFRTPWKLWATGNYVNGLREGEWTFRDSSGHNDMLITF
                     VNGKAYGQATFFYLFTGVKAESGFLDDCNRTGTWKNYDSLGNLSGEFNYVDGQKSGPC
                     TLYYPDGKIKAKGQYVQVSRKFVQICEDPNNPGTFTKCSYRYAAPAKNGTWFFYDNSG
                     NLTGTKKYHHETSESVSVQYYYNNKGKRTRVRTIGYDSDYCGKIW"
     gene            complement(1130..1936)
                     /locus_tag="VFU15_17015"
     CDS             complement(1130..1936)
                     /locus_tag="VFU15_17015"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEU4719549.1"
                     /translation="MTIFRKIFTETHHREQKQLAENPPHTGFDRKTAVILVWTALGLA
                     ITKYYGDTTFVSGVLKDWHLDSLDNSFRSWLDSNAHAKLHHLEWWVGSLIFVYFIVPV
                     IFIRFVFREKLSDYGLKRRGAFHSWPIYVLMLAIMLPLIYYFSSTASFQRRYPFYDPV
                     HGESLWPDFWIWELFYFSQFIALEFYFRGVMTLGLKHRFGYYSIFVMTIPYCMIHFGK
                     PMPETIGAIFAGIILGTLSMKSRSVWLGVAIHYSIALSMDMFSLWRKGFF"
     gene            complement(1933..2946)
                     /gene="tsaD"
                     /locus_tag="VFU15_17020"
     CDS             complement(1933..2946)
                     /gene="tsaD"
                     /locus_tag="VFU15_17020"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002996629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEU4719550.1"
                     /translation="MPVILGIESSCDETAAAVIDNGKLLANIVAGQEVHRQWGGVIPE
                     LASRAHQQHIVPVIDKTLKTAGKLPAEIDAVAFTRGPGLLGSLLVGTSFAKGFALALG
                     KPLIEVNHMRAHILAHFIEDEHPRAKPSFPFLCLTVSGGHTQIVKISDPYTFNILGET
                     IDDAAGEAFDKTAKILGLPYPGGPLIDQHAANGDPLAFHFPSSNVGDYNFSFSGMKTA
                     VLYFVRDELKKDEMFLQKRMDDLCASVQHTIVTALMKKLEKAAGDTGIRDIALAGGVS
                     ANSGLRKKLEETGKAKGWNVFIPPFSYCTDNAAMIAMAGHFKFLRHEFSGQDVAPLAR
                     YPV"
     gene            2966..7582
                     /locus_tag="VFU15_17025"
     CDS             2966..7582
                     /locus_tag="VFU15_17025"
                     /inference="COORDINATES: protein motif:HMM:NF016260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HEU4719551.1"
                     /translation="MAGKREIAVKIIKKTGRILAWTSGFLLIFLTSSYFLLRSPAVQT
                     LISRRAADYISGELNAKVAVKGVDVEFFSKIVLEGFYVQDRHGDTLLYADKFKADIQR
                     FSFSNRYIRTGNIDLDNSVVKLKKYKGEHGLSYRFLQDYFSGKPTKKKNPGAPWKVDL
                     GSVTLSSITFAYIDTRDTTPSRGMNYQDIRVSPVSCEFSRFNPMGDSLSVHIRNLSGK
                     EKCGFTLNSFSTILTAGDTFARFDNLKFFTPQSNVEGFISFSFHDKDDLSDDFIHLVK
                     MDGHFSSSILDMSDIAFFAPELIGIHKKILLTGDVNGTVDNLHCRNVDIQFGKKSRIA
                     GNFHFSGLPDISETDMNFSVRQARTDLEDLRSIPVPPFDQGKTLNVGDNIGLLGTIVF
                     SGSFEGFLNDFVAHGKVNTALGDLTLNNLAMFRDNDSLPMSYNGEVTASSFDIGKFLG
                     IDGVGKVTGNVKGKGSGLDEESVNANIDGHFSRFDFNNYSYTGITVTDGKLKRKSFDG
                     KIDMSDPNAKLKFKGFVDWSSPKKDYVFDFNARIDSADLGELGFMDKSHEYILSTNLA
                     MHFHGSNIDNIDGRLGVENLVYQKDGEKFAFNEIAVNAFHVNDSTQALSLSSDIASGN
                     ITGQFQFMQLPAAVSDIMSDYLPSYFPPKIYSKNEKKPLPQSFAWNVKFGQNTKPVKA
                     LVPDLDIAPGTYFAGNFDQRKRSLKTDFISDRIAYGDKTFSGIYLSSGNKEGYLSLTG
                     NLARVQLSDTVGASNISINCRANKDDLLANVVWDNSSKKKNSGNISAMAHFESPTSVK
                     MNLMQAQLYLNDSLWSVDPKNYVRVDSNTVTFHDLVFNSGKQAVGLNGIVSPRPEDIL
                     GVTVTNFNLAYLNYFTEPSGVTMTGTISSDTKLSDIEHTPIFTSNTTFSNFIVNDQKI
                     GDGIVSADWQKAKAAVYIHGQFTKGLINEDTKQPINNILFDGYYYPKDKENSLDINST
                     LTNIPLETLQPVMKDFCSKLVGQIDGNLHVGGTFSKPLLNGTMNVSIRRVIVDYLGLA
                     IGAPAQPITIEENSFFFDDYHVIDMYGDTATVYGHLYHDNFKKFQFDMDFGFDHFLVL
                     NTTAKQNDLYYGRVFATGYMNLFGYVDEKVSIDMSASTDKIYYNGKTINSDFNIPMTS
                     TSEVGENDFITFVSHSDLDSTKKNNRLRNNGIELNLNVQATPDALVHVIFDKTVGDEL
                     KASGTGNINMHITPAGEFTIFGNYEVEQGDYQFTMKNIPMAKFDLSKGGVLSWNGDPY
                     AATIDADAVYHANASVAPFFPLDSANTAYTRSYPVDVIMHLDSSLMNPSISFDVNLPT
                     ADQNIQETVKSYMPPNSLELNRQVLSLMVLNSFMTPTELREGNAANSNVVAGTSATLL
                     SNFVSGTLNNWLSQISSDVNMGVKYRPNDDLSPQELKVYLGTQLFNNRVTIDADAGKV
                     NQSVTTGTTGNAGQFVGEANVEYKVTSNGKVRLRAFNRANDNTVLNSNSPYTQGAGIF
                     YREEFESGAQLRKHYRDFIMGKNPNREKKATSQGNSKPSQDSAK"
BASE COUNT         1941 a         2037 c         2011 g         1770 t
ORIGIN      
        1 gcaggtgcgt tttgggaagt aattattttt ttcatctgga atggaattgg gaacagccgc
       61 gaatatacga acgaaggagt gaaagcaccg ggaagtccgt gttaccagat cttgccgcag
      121 taatcagaat cgtaaccgat cgtcctcacc cgggttcgtt ttcctttgtt gttgtaataa
      181 tactgcacgc ttaccgattc actggtttca tggtgatatt ttttggtccc ggtgaggttc
      241 ccgctgttat cgtaaaaaaa ccaggtgccg tttttggcgg gggcagcata gcggtaactg
      301 catttcgtaa atgttcccgg gttgttcggg tcctcgcaga tctgcacgaa ttttctgctg
      361 acctgcacat attgtccctt cgcttttatt tttccgtccg ggtagtacag cgtgcacggt
      421 cccgatttct gcccgtcgac gtaattgaat tctcctgaaa gatttccgag cgaatcatag
      481 tttttccacg tgcctgtgcg gttgcagtcg tcgagaaaac cggattctgc tttcacgccc
      541 gtgaacaaat aaaaaaacgt ggcctgcccg tacgctttcc cgttgacgaa cgtgatcagc
      601 atatcgttgt gaccgctgct gtcgcggaac gtccattctc cttcgcgcag cccgttcacg
      661 taattcccgg tggcccacaa tttccagggc gtcctgaatt cgtagaaatg ataataggaa
      721 accgcgtagc cattatacag gctgtctttc acacggtaga tcgtgctgtc ggtgccggag
      781 taaacataga tcccgttccc gttcttcagc gtctgcgtgc cgtcttggag ccacgcgttc
      841 accagcttgt gcacccggaa cgaagtgtcc gtaatttctt ccaggcgctt tgttccgttt
      901 tcgtaataaa acgtccaccg gtcgtacccg ccgccgtccg tgtaacggta atgaatgccc
      961 gtccagtaga ttgttccgtt atcggaacgg ctggtatcat agctgatctt ttgtgccgga
     1021 gacagcaggg aaaaaagcag gaatgaaaaa aggagagtcg ctttcatgcc ggtcgtttga
     1081 tgctatgcct gtcttaatgc cggaaaagtg ttccggttac acacgggttt caaaaaaatc
     1141 cttttctcca gagcgagaac atgtccatcg agagcgcaat gctgtaatgg atggcgacgc
     1201 ccagccacac cgaacggctt ttcatgctca gcgttccgag aatgatcccg gcgaagatcg
     1261 cgccgatggt ttccggcatg ggtttcccga aatggatcat gcaataggga atggtcatca
     1321 cgaaaatgct gtaatacccg aaacggtgct tcagcccgag cgtcatcaca ccgcggaaat
     1381 aaaattcaag cgcgatgaac tgcgaaaaat agaacagttc ccatatccag aaatccggcc
     1441 agagactttc gccgtgaaca ggatcgtaga acgggtaacg gcgctggaag ctcgctgtgg
     1501 aggagaaata ataaatgagc gggagcatga tcgccagcat gagcacgtaa atgggccagc
     1561 tgtgaaatgc gccccggcgt ttcagtccgt agtcggataa tttttcacgg aaaacaaaac
     1621 ggatgaaaat gacgggcacg atgaaataaa cgaagatgag cgagcctacc caccattcga
     1681 ggtgatggag cttcgcgtgc gcattgctgt cgagccagct gcggaatgaa ttgtccagcg
     1741 aatcgagatg ccagtctttc agcacgccgg aaacgaacgt ggtatcgcca tagtatttcg
     1801 tgatcgcgag accgagcgct gtccatacga ggatcaccgc ggttttccgg tcgaaacccg
     1861 tgtgcggcgg attttccgca agctgtttct gctcccggtg gtgcgtttca gtgaagatct
     1921 tccggaaaat ggtcatacgg gatagcgtgc aagcggggcc acgtcctgtc ccgaaaattc
     1981 atggcgtaaa aatttgaaat gcccggccat ggcgatcatc gcggcattgt cggtgcaata
     2041 gctgaacggg ggaatgaaca cgttccagcc tttcgctttt cccgtttctt ccagcttttt
     2101 ccgcagtccc gaattggctg aaacgccgcc cgcgagcgca atgtcgcgga tgcccgtgtc
     2161 tcctgccgct ttttccagct ttttcataag cgcggttact attgtgtgct ggacagaagc
     2221 gcagaggtcg tccatccgct tctgcaggaa catttcatcc tttttcagct cgtcgcggac
     2281 aaaatacagc accgcggttt tcatgccgct gaagctgaag ttataatcgc ccacgttcga
     2341 agacgggaaa tggaaagcga gcgggtcccc gttcgccgca tgctggtcga tgagcggacc
     2401 gccggggtag ggaagcccga gaattttcgc tgttttgtcg aatgcttcac cggcggcatc
     2461 gtcgatcgtt tccccaagaa tgttaaaagt atacggatcg gaaattttaa caatctgcgt
     2521 gtgcccgccc gaaacggtga ggcagaggaa cggaaaagag ggttttgcgc gcgggtgttc
     2581 atcttcgatg aaatgcgcga gaatgtgcgc acgcatgtgg ttgacctcga tcagcggttt
     2641 cccgagcgca agcgcgaaac ccttggcaaa cgaggttccc acgagcagcg agcccagcaa
     2701 tcccggtccg cgcgtgaacg ccaccgcgtc gatttccgcg ggcaacttcc cggccgtttt
     2761 cagcgtcttg tctataacgg gaacaatatg ctgctggtgc gcacgcgatg cgagctccgg
     2821 gatcacaccg ccccactggc ggtgaacctc ctgcccagcc acaatgttgg caagcaattt
     2881 gccgttatcg ataacagcgg ctgcggtctc gtcacacgac gactcgattc caaggataac
     2941 aggcattatc gctacttttg ttacaatggc cggaaagcgg gaaatagctg tcaaaattat
     3001 caaaaagacc gggcgcattc tcgcatggac atccggcttc ctgctcattt tcctcacttc
     3061 gtcctatttc ctgctccgca gcccggccgt gcagacactc atcagccgca gggccgccga
     3121 ttatatttcc ggtgagctga acgcgaaagt ggcggtgaaa ggcgtggatg ttgaattctt
     3181 ctcgaaaatc gtcctcgaag gattttacgt gcaggaccgg catggagaca cactgcttta
     3241 cgccgacaag ttcaaagccg atatccagcg gttcagcttc agcaaccggt acatacggac
     3301 gggaaacatc gacctggaca attccgtcgt aaagctgaaa aaatacaagg gcgagcacgg
     3361 tctcagctac cgtttcctgc aggattattt ttcaggtaag ccgacgaaga agaaaaatcc
     3421 gggcgcgccc tggaaagtgg acctcggttc tgtcacgctt tcttccatca cgtttgcgta
     3481 catcgacaca cgcgacacga cgccttcgcg cggaatgaac taccaggata tccgcgtttc
     3541 cccggtcagc tgtgaatttt cccgcttcaa cccgatgggc gattcgcttt cggtgcacat
     3601 ccgcaacttg tccgggaaag aaaaatgcgg cttcacgctc aattccttca gcacgatact
     3661 caccgccggc gatacgttcg cgcgcttcga caacctgaaa ttcttcacgc cgcaaagcaa
     3721 cgtcgaagga ttcatttcat tttcgttcca tgacaaggac gacctttccg atgatttcat
     3781 tcacctggtg aaaatggacg ggcatttttc gtcttccatc ctcgatatgt cagacatcgc
     3841 ctttttcgcg ccggaactca tcggcataca caaaaagata ctgctgaccg gggacgtcaa
     3901 cggaacggtg gataatcttc attgccgtaa cgtcgacatc cagttcggga agaaatcccg
     3961 cattgccggg aattttcatt tcagcgggct gccggacatt tcagaaacgg atatgaattt
     4021 cagcgtgcgg caggcgagga ccgatctcga agacctgcgc agcattcccg ttccgccctt
     4081 cgaccaggga aaaacattga atgtcggcga taacatcggc ctgctcggca cgatcgtttt
     4141 ttcaggatcg ttcgaagggt tcctgaatga tttcgtggcg catggaaaag tgaacaccgc
     4201 gctcggcgat cttacgctga acaacctcgc gatgttccgc gataacgaca gcctgcccat
     4261 gtcgtacaac ggcgaagtga cggccagctc attcgatatc gggaaatttc tcggcatcga
     4321 cggcgtggga aaagtcaccg gcaatgtgaa aggaaaaggc tccggtctcg acgaggaatc
     4381 cgtgaatgcg aatatcgacg gtcatttttc ccgtttcgat ttcaacaatt attcatacac
     4441 gggaattacg gtcacagacg gaaagctgaa gcggaaatcg tttgacggga agatcgacat
     4501 gagcgatccg aacgcgaagc tgaaattcaa aggcttcgtc gactggagca gcccgaaaaa
     4561 agattacgtg ttcgatttca acgcccgcat cgattcggct gatctcggtg agctcgggtt
     4621 catggacaaa tcgcatgaat atattttgtc gaccaacctg gcgatgcatt ttcacggaag
     4681 caatattgac aatatcgacg ggcggcttgg cgtggaaaac ctggtgtacc agaaggacgg
     4741 tgaaaaattc gcgttcaacg aaattgccgt aaacgctttc catgtcaacg attcgacgca
     4801 ggccctgtcg ctttcttcgg atattgccag cgggaacatc accggccagt tccagttcat
     4861 gcagcttccc gccgccgttt ctgatatcat gtccgattat cttccttcgt atttcccgcc
     4921 gaagatctac agtaagaatg aaaagaaacc tttaccgcag agctttgcct ggaacgttaa
     4981 gttcgggcag aacacgaagc cggtaaaagc gctcgtgcct gatctcgata ttgcgccggg
     5041 aacttatttt gcaggaaatt tcgatcagcg gaaacgctcg ctcaaaacgg attttatttc
     5101 cgaccggatc gcttacggag ataaaacatt ttccggcatc tatctttctt cgggcaataa
     5161 ggaaggctac ctgagtctca cgggaaatct tgcccgcgtc cagctttcgg atacggtcgg
     5221 tgcaagcaac atttccatca attgccgggc gaacaaagac gacctgcttg cgaatgttgt
     5281 ctgggacaac agctcgaaga aaaagaattc gggcaacatc agcgcaatgg cgcattttga
     5341 aagtccgaca tccgtaaaga tgaacctgat gcaggcgcag ctttacctga acgattcgct
     5401 ctggagcgtc gacccgaaaa attacgtccg cgtcgattcg aataccgtca cgttccatga
     5461 tcttgttttc aattcgggca agcaggcggt cggactgaac gggatcgttt ctccccggcc
     5521 ggaagatatt ctcggcgtta cggtgaccaa tttcaacctt gcgtacctga attatttcac
     5581 ggaaccttcc ggcgtgacga tgacaggaac catctcatcc gatacaaagc tttcggacat
     5641 cgagcacacg ccgatcttca ccagcaatac cacgttcagc aatttcatcg tgaacgacca
     5701 gaaaataggc gacggtattg tgagcgccga ctggcagaaa gccaaagccg ctgtatacat
     5761 ccacgggcaa ttcacgaaag gactcatcaa cgaagacacg aagcagccga tcaacaacat
     5821 cctgttcgac ggttattatt acccgaaaga caaggagaat tcgctcgaca tcaattccac
     5881 gctgaccaac attccgctgg aaacgcttca gcccgtgatg aaagatttct gctcgaagct
     5941 cgtcgggcag atcgacggga acctgcatgt gggcggaaca ttttcgaaac cgctgctgaa
     6001 cgggacgatg aacgtttcca tccgtcgcgt gatcgtcgat tacctcgggc tggccatcgg
     6061 cgcgccggcg caaccgatca cgatcgagga aaattctttc ttcttcgatg attaccacgt
     6121 catcgacatg tacggcgata cggcgacggt gtacggtcac ctgtatcacg ataacttcaa
     6181 gaagttccag ttcgacatgg acttcggctt cgatcatttc ctcgtgctga atacgacggc
     6241 caaacagaat gatctttact acggccgggt tttcgcgacg ggttacatga acctgttcgg
     6301 ttatgtggat gaaaaagtca gcatcgacat gagcgcgagc acggataaaa tttattacaa
     6361 cgggaaaacg atcaactctg atttcaacat cccgatgacg tcgacgagcg aagtggggga
     6421 gaacgatttc atcacgttcg tcagccacag cgatctcgat tcaacgaaaa agaacaaccg
     6481 tctccgcaac aacggcatcg agctgaacct gaatgtgcag gccacgcccg atgcgctggt
     6541 gcacgtgatc ttcgacaaga ccgtgggcga tgagctgaaa gcgtcgggaa ccggtaacat
     6601 caacatgcac attacaccgg cgggagaatt caccattttc ggcaattatg aagtagagca
     6661 gggggattac cagttcacca tgaaaaatat tcccatggcg aagttcgatc tttcgaaagg
     6721 aggcgtgctg agctggaacg gcgatccgta tgccgccacg atcgatgcag atgcagtgta
     6781 ccatgcgaat gcaagcgtgg caccattctt cccgctcgac tcggccaaca cagcttacac
     6841 acggtcttac ccggtggacg tgatcatgca cctcgacagc agcctcatga acccgtccat
     6901 atcgtttgat gtaaaccttc cgacagccga ccagaacatc caggaaacgg tgaaaagcta
     6961 catgccgccg aactcgctcg agctgaacag gcaggtgctt tcgctcatgg tgctgaacag
     7021 cttcatgaca ccgacggagc tgcgcgaagg aaacgccgcc aattcgaacg tggtggcggg
     7081 aacgagcgcg acgctgctca gtaatttcgt cagcggaacg ctgaacaact ggctttcaca
     7141 gatcagcagc gacgtgaaca tgggcgtgaa ataccgtccg aacgacgacc tgagtccgca
     7201 ggagctgaag gtgtatctcg gcacgcagct tttcaataac cgtgtgacca ttgatgcgga
     7261 cgccggcaaa gtgaaccaga gcgtgaccac gggtaccacc gggaacgcag gacagttcgt
     7321 gggagaagcc aacgtggaat acaaggtcac ttccaacggg aaagtgaggt tgcgcgcgtt
     7381 caaccgtgcg aacgataata ccgtgctgaa ttccaactca ccgtacacgc agggcgcagg
     7441 tattttttac cgggaagaat tcgaaagcgg cgcgcagctc cgcaagcatt accgtgattt
     7501 tatcatggga aaaaatccca accgggaaaa gaaagccact tcgcagggaa acagcaagcc
     7561 atcgcaggat tccgctaaat aacaatgccg gattctcaat gattcaaccg gcgtttttct
     7621 ttgtgaactt cgtgaaaaag acttcgtggc ctttgtgttg aaatggaaat aatcagcaag
     7681 ccgtcgcaga actccactaa ataacaacgc tgaactctca atgattcaac cagcgttttt
     7741 ctttgtgaac ttcgtgaaa
//
LOCUS       DASTSF010000603         3034 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_123809,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000603 DASTSF010000000
VERSION     DASTSF010000603.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3034
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123809"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            3..665
                     /locus_tag="VFU15_17030"
     CDS             3..665
                     /locus_tag="VFU15_17030"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009416922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEU4719552.1"
                     /translation="MLVVAVHPDDETLGAGGTLFRHKANGDSVAWLIVTSMHERQGFS
                     PERIRERDAEISSVSKAFGMEHVFRLDFPTSELSSSSLLQLVPAISKIFHEYQPSVIY
                     LPNRSDAHSDHRITFDAVMACTKSFRYPSIREVLMYECLSETEFGAPLPENSFIPNYY
                     VDISEFLTKKNEVLKLFRSEMGEHPFPRSARNVEALATFRGATAGVEYAEAFSVVKII
                     HK"
     gene            673..1323
                     /locus_tag="VFU15_17035"
     CDS             673..1323
                     /locus_tag="VFU15_17035"
                     /inference="COORDINATES: protein motif:HMM:TIGR03570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEU4719553.1"
                     /translation="MESAKPVLLLGYSGHAYVVCDALLSSGKKIAGYLEKESKNENPY
                     GLDYFGSEEDNDVMKKITGMDYFVAVGDNSLRRKIADGMKKKMNAGAVNAVHARAVIS
                     SSAELADGILCAPVSIVNAKAVIGNGVILNTACVVEHECIVGDFAHIAPGAILCGNVR
                     VGEGTFVGAGAVVREGIVIGKNSLIGAGTVVNRDVPDNVKVVGNPYRIIGVYELER"
     gene            1307..2359
                     /locus_tag="VFU15_17040"
     CDS             1307..2359
                     /locus_tag="VFU15_17040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEU4719554.1"
                     /translation="MNLKDKLTISRDEPARNALQRLTDLAMPGAALFVLDKEGRVVGT
                     LTDGDIRRALLGGSDVKTPLSEIANANFRFLTEARPGAVFIDECRRFNIRYLPVLNAQ
                     QQLLSIIDLNSIKGILPVEAVLMAGGKGERLRPLTDDTPKPLLKVGGRPIIERNIERL
                     ISYSISRFHITVRYLGEKIERYLGDGSTYNAVFSYLHEDHPLGTIGALRSIGSFSEEN
                     ILVMNSDLLTNIDFGDFYNFFVESGADMVVATVPYHVDIPYAVLETTDEQEVLSFLEK
                     PRYTYYSNAGIYLLKKKLVDLVPANEKFDATDLMEAVIAKGYKLKSYPVVGYWLDIGR
                     MEDYRKAQEDVKHIQF"
     gene            2361..>3034
                     /locus_tag="VFU15_17045"
     CDS             2361..>3034
                     /locus_tag="VFU15_17045"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016478173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HEU4719555.1"
                     /translation="MSNTGNILYFIPARGGSKGLPGKNIRMMNGKPLIAWSIGTALAA
                     AAQTGGRVVVSTDSEEIADIARKAGAEVPFLRPAELASDKAPTIDVITHALDHFRKEG
                     IEFHLFCLLEATSPQRDVKDITAAYDLLLKTKSAESVVGVCRAESSHPAFLVTKDSSG
                     FIKPYGSDTFVVKRRQDIGDVYFYEGSLYISYTASLLANRSFYHAKTIGYEMPKWKSF
                     EVDDIV"
BASE COUNT          743 a          749 c          842 g          700 t
ORIGIN      
        1 aggtgctggt ggtggccgtt catcccgacg acgaaacgct cggtgccggc ggaactttgt
       61 tcaggcataa ggccaacgga gattctgtcg cctggcttat cgttacatcc atgcacgaga
      121 gacagggttt ttcccccgaa cgcatccggg aaagggacgc cgagataagt tcggtttcga
      181 aagcattcgg aatggagcat gtgttcaggc ttgattttcc tacgagcgaa ttgtcttctt
      241 catcgctgct tcagctggtt cccgccattt caaagatttt tcatgaatat cagccgtctg
      301 taatttatct gcccaaccgc tccgacgcgc attccgatca caggatcaca tttgacgctg
      361 tgatggcctg cacaaaatcg ttccggtatc cttctatacg ggaggtgctc atgtatgaat
      421 gcctttcgga gacggaattc ggggccccgc tgcctgagaa ttcatttatc ccgaattact
      481 acgtcgacat atctgaattc cttacgaaga aaaatgaagt gctgaaatta ttccggtcgg
      541 aaatgggaga acacccgttc ccgcggagcg ccaggaatgt cgaagcgctc gcaacattca
      601 ggggtgcgac agcaggtgtg gaatatgcgg aagcattttc ggtggtaaag atcatccata
      661 aataaaaaaa cagtggaaag cgcaaaacct gtcctgttac tcggttactc cggccatgca
      721 tacgtggtat gcgatgcgct gctgtcgtcg ggaaaaaaga tagccggtta cctcgaaaag
      781 gaatcgaaaa atgaaaaccc ttacgggctt gactatttcg gttctgaaga agataacgat
      841 gtgatgaaga aaataaccgg catggattat ttcgtggccg tcggtgataa ttccctgcgc
      901 aggaagattg cggatggcat gaagaaaaag atgaatgcgg gggcggtgaa cgcggttcat
      961 gccagggcgg tgatttcttc ttctgcagaa ttagcggacg gtattctgtg cgcgcccgta
     1021 tcgatcgtca acgcgaaagc ggtgatcggg aacggggtta ttctcaatac ggcgtgcgtg
     1081 gtcgagcacg aatgtatcgt gggtgatttc gcgcacatcg ccccgggtgc gatcctgtgc
     1141 gggaatgtgc gcgtaggaga aggaacattc gtcggggcgg gcgctgtggt ccgcgaaggg
     1201 atcgtgatcg ggaaaaattc attgatcggc gcaggaacgg tcgtcaaccg cgatgtgccg
     1261 gataatgtga aggtcgtcgg aaacccatac cgcattatcg gagtgtatga acttgaaaga
     1321 taaactgacg atcagccgcg atgagcctgc acgcaatgcg ctgcagcggc ttacggatct
     1381 cgccatgccg ggagccgctc tttttgtgct ggataaggaa ggacgcgttg tcggaacgct
     1441 tacggatgga gatattcgcc gtgcgcttct cgggggatcg gatgtcaaaa ccccgctttc
     1501 ggagatcgcc aatgccaatt tccggtttct gacagaagca cggccaggcg cagttttcat
     1561 tgacgaatgc aggcggttta atatccgtta cctgcccgtt ctgaatgcgc agcagcagct
     1621 gctgtcgatc atcgatctca attcgatcaa aggcattctg ccggtcgaag cagtgctcat
     1681 ggccggaggc aaaggtgaac gtctccgccc gctgaccgac gatacgccaa aaccgttact
     1741 gaaagtaggc ggcaggccga tcatcgaacg caacatcgaa cggctcattt cgtacagcat
     1801 ttccaggttt cacatcacgg ttcgctatct cggtgaaaaa atcgaacgat acctcggcga
     1861 cggttcaacc tacaatgccg ttttttccta cctgcacgaa gatcatcccc tcggcacgat
     1921 cggcgcgctg cgcagcatcg ggagcttttc ggaagaaaat attctcgtaa tgaattccga
     1981 cctgctcacg aatatcgact tcggtgattt ttataatttt ttcgtggagt cgggagcgga
     2041 tatggttgtg gccacggtgc cttatcatgt cgatattccg tatgctgttc ttgaaacaac
     2101 agacgagcag gaggtgctct ccttcctcga gaaaccacgc tacacttatt attccaatgc
     2161 gggaatttac ctgctgaaaa aaaagctggt ggacctggtc ccggccaatg agaaattcga
     2221 tgcgacggac ctgatggagg ctgttatcgc gaaaggatac aagctgaaaa gctatcctgt
     2281 tgtgggctac tggctcgaca tcgggcgaat ggaagattac cgcaaagcgc aggaagacgt
     2341 aaagcacatc cagttctgaa atgagcaaca ccgggaacat cctgtatttt attcctgccc
     2401 gcggcggaag caaaggcctt cccgggaaaa acatccgcat gatgaacgga aagccgctca
     2461 tcgcatggtc gatcggaacg gcgctggcag ctgcggcgca aacgggaggc cgcgtggttg
     2521 tttccaccga cagcgaagag atcgcggaca tcgcgcggaa agccggtgcg gaagttcctt
     2581 tcctgcgacc cgctgaactg gcttcggaca aagcgcccac catcgatgtc attacgcatg
     2641 cgctggacca cttcaggaag gaaggaattg aattccatct cttttgcctt ctcgaggcaa
     2701 cgtctccgca gcgcgacgtg aaagatatta ccgctgctta cgatctgctc ctgaaaacaa
     2761 aaagtgcgga aagtgttgtc ggcgtctgcc gcgctgaatc atcgcatcct gcatttcttg
     2821 tcacgaaaga cagcagcggg ttcataaaac cgtatggctc cgatacgttc gtcgtgaagc
     2881 ggaggcagga tatcggcgac gtttattttt acgagggaag cctttacatt tcatacacgg
     2941 cttcgctgct cgccaaccgg tctttttacc acgcgaaaac gatcggctat gaaatgccga
     3001 aatggaaatc attcgaggtc gacgatatcg tcga
//
LOCUS       DASTSF010000604         9530 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_885078,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000604 DASTSF010000000
VERSION     DASTSF010000604.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 9530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9530
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_885078"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(56..1498)
                     /locus_tag="VFU15_17050"
     CDS             complement(56..1498)
                     /locus_tag="VFU15_17050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011551042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HEU4719556.1"
                     /translation="MEKLLNYINGKLEAPASGNYIDNYQPATGKVYSLIPDSDAADVQ
                     KAVDAAKAAFPQWSVTPVEKRAKLMMKIASLIERDMDAFVKAESKDNGKTVHTAGLID
                     IPRAISNLEFFATGIMHYAAEAHATEDTAINYTLRQPIGVVGCISPWNLPLYLFTWKI
                     APAIAAGNCVVAKPSEITPVTAFLLSKACIEAGLPAGVLNIVHGLGGKTGQAIIEHPE
                     IKAISFTGGTKTGATIAATAAPKFKKLSLELGGKNPNIIFADCDFDAMLKTTIQSSFA
                     NQGEICLCGSRIFVERPLYEKFRTAFVEKTKELRPGDPSDPKSRMGAIVSQQHMEKIL
                     SYIELAKKEGGKVLCGGNRVEMPGDLKDGWYIEPTIIEGLSYDCRTNQEEIFGPVVTI
                     TPFDTEEEVLKMANSTMYGLAASVWTQHLTRAHRMAARIQSGIIWINCWLLRDLRTPF
                     GGMKGSGVGREGGFEALRFFTEPKNVCIKL"
     gene            complement(1552..2283)
                     /locus_tag="VFU15_17055"
     CDS             complement(1552..2283)
                     /locus_tag="VFU15_17055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719557.1"
                     /translation="MIRPFFILAFFLSAKILSAQDTLHGDTALFEKMYAKLQHQVDQY
                     HTAEERDSLTALQDSLLVMFFNDSSTFNYHFSFCNAIRYAGDLQVMTMEDNTDGPFRS
                     VTTCMRYRGESGAIHFTMLDGAVTEEEQDNFLCTGVYCLNREQKIYLVTGTGMSCNSC
                     PFACARTYEISGDDLVEYPAFAGSSVLSMSWNGKDCNAGVTYDETGKELTLSYCDVIP
                     GGSGKQQQYTDRYRFGVIVFVKEGK"
     gene            2350..2772
                     /locus_tag="VFU15_17060"
     CDS             2350..2772
                     /locus_tag="VFU15_17060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020400109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3052 domain-containing protein"
                     /protein_id="HEU4719558.1"
                     /translation="MAKTPGYSGTPLAKKLGIKENFRVQLIAQPEHYSALFDDLPEIR
                     IVNDAKTPRDLVHFFVTKTAELEKKIPALKKTLAPAGMIWVSWPKKSSGVATDVTEDV
                     IRSIALKNGLVDIKVCAIDETWSGLKLVIPVKDRKKLT"
     gene            2831..3448
                     /locus_tag="VFU15_17065"
     CDS             2831..3448
                     /locus_tag="VFU15_17065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719559.1"
                     /translation="MRLRFLPVFFLPGLFFLAACSSNPGPKKDIVSKYIGVLPTVVDN
                     DEGLFHGIMLGMTPEEVKAHAISRDSLGEEKPGYLMFQGRISGKEEYLYDCQFDTNGL
                     FTLELDIYLKDKPSGDSIFSDFKSYYTARFGTPQEEKGMLIWTCNEGTRPAKIIIDED
                     PAYSYGELTVTIYDKTFDTPPPGVSDSLNGNYSDSLDGKDTLILP"
     gene            3533..5113
                     /locus_tag="VFU15_17070"
     CDS             3533..5113
                     /locus_tag="VFU15_17070"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEU4719560.1"
                     /translation="MKQFRTRIAIVAIFLLIVLPAAAQHTFFTLLNGYTSMPTCIQQT
                     ADGNYFVTAHGNQYFLMNDTGSLAWEKNLYDPNLYFDCQYNLPTPDHGFIFSGPVTDP
                     VLGSYGYIAKLDSSGHVQWMRMTGLADDPMGYSMCSIAASPDSGYVFAAGVNVTTAAS
                     PKRGALVMKLGANGNTDWSRVIVPDTAYQPYRVQSTCIKALPGGDYIVAGLADFTLLL
                     CRLDSAGNLRWVKTYLQDSLAGQTVYVDTVSDGGFVVSANLDLAQSNQYQACLLKTDS
                     TGNPQWCRLYGTPGQIEIKCVRTLAGGGYAFMGMENTSPWQGAALVKTDPSGNPVWVS
                     KFDSVFIWQTIKWSKYQFVQTSDLGFVFGGLVISQQTGQTYPCMLKTDPQGMTACRGH
                     QVSISTQTLPVSVDTIGYEEPGESPSPFSRQEQADIITDSLICSGVFTVNTEGIEEAS
                     ASPGVLLYPNPACDLLQVTAGMSCTFELYNSAGQLLQKKAAGPGTVQFDIHAYPPGLY
                     FLVTDTGTGRTASKFIRE"
     gene            5193..7286
                     /locus_tag="VFU15_17075"
     CDS             5193..7286
                     /locus_tag="VFU15_17075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018341979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU4719561.1"
                     /translation="MESSKAETEETTRVNHPSGVLLEVRNLVTEFRTENETVKAVNDI
                     SFSIKAGETIGIVGESGSGKSVTSLSVMRLIPNPPGEITGGEVLFTSRNDGVIDLLQM
                     PEKKMQKYRGNEIAMIFQEPMTSLNPVYSCGEQVMETILQHWKSPSIPDSRNFIEKTI
                     RSLYKFMLVFGSIFIGCMMFYLLFSDKSFTGKLLLILSTLYFTGLFTFYFFRKRAGVY
                     RPFHYYTSRERVAKKNTLDLFGQVRLPDPERIFYSYPHQISGGQKQRVMIATAMACNP
                     QLLIADEPTTALDVTVQKTILELMRDLQAEHNMSIMFITHDLGVIAELADRVVVMYKG
                     KIVEQGSVLDIFSNPQHPYTKSLLACRPPLNKRLQWLPTVSDFMSVDNAGEMVENSRS
                     VLEVTHRLEVTKEDRKRQHSELYNREPILKLNNIRTHFVTQRNAFGKPVHVVRAVDDI
                     SFDVYPGETLGLVGESGCGKTTLGRTILRLIEPTGGTIIYKGKDITKLSAHDMRLLRK
                     DIQFIFQDPYSSLNPRITIGDAIMEPMKVHNIGKSEKDRKERVIELLHRVNMNETHFY
                     RYPHEFSGGQRQRICIARALALNPQFIICDESVSALDVSVQAQVLNLLNELKKEFKFT
                     YIFISHDLSVVKFMSDRMVVMNEGKIEEMGEADMIYNDPQKEYTKKLINAIPKGELDD
                     IRASIARKRMRAQAF"
     gene            7314..8705
                     /locus_tag="VFU15_17080"
     CDS             7314..8705
                     /locus_tag="VFU15_17080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719562.1"
                     /translation="MKKRWPFLFFPATFLLLWILALLSKGIYDPGDGVMHYLFAHYAG
                     KHPALFLDHWAKPLYTLLSFPFAVFGYGGTLAFNLLCLCASGWFTWKIAEKLKLPFAP
                     LAAPLVIFAPIALPVGMSGLTEPLFMLVLVAGIFFVMNGRNGIAALVISLLPFARTEG
                     FFLAPLFAFYFLGRKDFVPVFLLGAGTLLYSVAGAFLYHDILWIIHKNPYTGAEKIYG
                     HGTLFHFIALNEFTWGWGMTALLAMGMIAAVFRKRFAASVSSAEGWLVYGTFLLFLVM
                     HSVFWWKGLFGSLGLHRVMACTIPSAVLISLRGFHVIGAYVRSAAAMKTIAGIVLAVQ
                     VFLTWRQHPLPLQPSDDFAIDRTAADFLAENNYTGERIFSSHPVFAFLAGKDPFDKTQ
                     FVSFTCSCLDTDFRKGDIVIWDSHFTKFDYGIPIEELSNHPKLKELARFGEMPATREE
                     ASGKNLVGIFQVK"
     gene            complement(8833..>9530)
                     /locus_tag="VFU15_17085"
     CDS             complement(8833..>9530)
                     /locus_tag="VFU15_17085"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU4719563.1"
                     /translation="DITNVIRYFMDEWIPPVIRDSRWFMYPFYMFAYRGKNIREVMDF
                     KSRVHSFTPEDYDHFYNNLDTISRNRVTDLNKPSLRFILDGIDPSAQTLIDVGSGNGF
                     LLKEIHRRHPQLKLTGFDIKDSDDNADVYSYVKGNIERMPFPDKSFDVVTCSHTVEHL
                     LKLDQCMEELVRIARKQVFIVTPCQRYYYYTLDEHVNFFQHERELTSHIPLKDYTCTK
                     LKGDWVYLGKIPQ"
BASE COUNT         2111 a         2676 c         2534 g         2209 t
ORIGIN      
        1 gcaccgcgca cgcgtttcta tgctggtccg ccggtgatgc ggcgaaaatt tccgcttaca
       61 gctttatgca gacgttcttc ggctccgtga agaatcgcag cgcttcgaaa ccgccttcac
      121 gtcccacgcc ggaacctttc atgccgccga acggcgtgcg caggtcgcgc agcagccagc
      181 agttgatcca gatgatcccg ctctgtatgc gcgcagccat gcggtgcgcg cgcgtaaggt
      241 gctgtgtcca gacggaagca gcgagcccgt acatcgtgct gttcgccatt ttcagcactt
      301 cctcttccgt gtcgaacggc gtgatggtaa cgaccggtcc gaaaatttct tcctggttcg
      361 tgcgacagtc gtacgacaat ccttcgatga tcgtcggctc gatgtaccag ccgtctttca
      421 ggtcgccggg catttccacg cggttcccgc cgcagaggac cttcccgcct tctttcttcg
      481 ccagctctat gtaggaaaga attttctcca tgtgctgctg cgaaacaatg gcgcccatgc
      541 gcgatttcgg atcggaagga tcgccgggac gaagctcttt ggttttttca acgaaagccg
      601 tcctgaattt ttcatacagc gggcgctcca cgaaaatgcg cgagccgcac aggcagatct
      661 cgccctggtt ggcgaaggaa gattgaatcg tcgtcttcag catcgcgtcg aaatcacaat
      721 cggcgaaaat gatgttcggg tttttcccgc cgagttcgag ggaaagcttt ttgaatttag
      781 gagcagctgt ggcggcgatc gtcgccccgg tttttgttcc gccggtgaac gagatcgctt
      841 ttatttcagg atgctcgatg atggcctgcc cggtttttcc gccgagtccg tgtacgatgt
      901 tcagcacgcc tgcgggaagt ccggcttcga tgcacgcttt cgacaacagg aacgccgtca
      961 ccggtgtgat ctcggatggt ttcgccacaa cgcagttgcc cgctgcgatg gccggcgcga
     1021 tcttccacgt gaaaagatac agcgggaggt tccacgggga aatgcagccc accacgccga
     1081 tgggctggcg cagcgtataa ttgatcgccg tgtcttccgt tgcatgcgct tccgccgcgt
     1141 aatgcatgat gcctgttgca aaaaattcaa gattgctgat ggcgcgcggg atgtcgatga
     1201 gcccggcggt gtgcacggtt ttgccgttat ccttcgattc cgctttcacg aacgcatcca
     1261 tgtcgcgttc gatgagggac gcgatcttca tcatcagctt cgcgcgtttt tcaaccggtg
     1321 taacggacca ctgcgggaac gccgctttcg ctgcatcgac ggctttctgc acgtcggctg
     1381 catccgaatc gggaatgagc gaatacactt ttcccgttgc gggctggtag ttatcgatat
     1441 aatttccgga agcaggcgct tcgagctttc cgttgatgta gttgagcagc ttttccatga
     1501 tgaatgcaaa atgagaagca caaagttacc gggatttccg cttcagcacg ttcactttcc
     1561 ttccttcaca aatacgatca cgccgaaccg gtagcggtcc gtatactgct gttgttttcc
     1621 gctgccgccg gggatgacgt cacaatagct gagcgtaagc tcttttcctg tttcatcgta
     1681 cgtcactcct gcattacagt ctttgccgtt ccagctcatg gacaggacgg aggacccggc
     1741 gaatgcagga tattccacga gatcatcacc ggaaatttca tacgtgcggg cgcatgcgaa
     1801 cgggcagctg ttgcagctca tgccggtgcc cgtgacgagg taaattttct gctcccggtt
     1861 cagacagtat acgcccgtac acaaaaagtt atcctgctct tcctccgtga cagcgccgtc
     1921 gagcatcgtg aaatggatgg cgcccgattc tcccctgtac cgcatgcagg tggtgacaga
     1981 acgaaaagga ccgtccgtat tgtcctccat cgtcatcacc tggagatcgc ccgcatagcg
     2041 aatggcattg cagaaactga aatgataatt gaatgtcgaa gaatcattga agaacatcac
     2101 cagcaggctg tcctgcaaag cggtgagcga atctctttcc tccgccgtgt gatactggtc
     2161 gacctggtgc tgcagcttcg cgtacatttt ttcaaacagc gccgtatctc cgtgcaacgt
     2221 gtcctgtgcg gaaaggattt tcgctgacag gaaaaaggca aggatgaaaa acgggcggat
     2281 catgggaagg gtttctgaat ccggtcaaag ataatgcagg ccggaaggaa gcgctaactt
     2341 tcggggatca tggccaaaac acctggatat tcgggcactc cgctggcgaa gaagctcggc
     2401 atcaaagaaa atttccgcgt gcagctgatc gcgcagccgg aacattattc tgcgctgttc
     2461 gacgatctgc cggagatccg catcgtgaac gacgcaaaaa caccacgcga cctggtccat
     2521 ttcttcgtca cgaaaacggc tgagctggag aaaaagatcc cggcgctgaa aaaaacgctt
     2581 gccccggcgg gaatgatctg ggtttcgtgg ccgaaaaaat cttcgggtgt tgcaacggac
     2641 gttacggaag acgtgatccg cagcattgca ctgaagaacg ggctcgtgga tattaaagtc
     2701 tgcgccatcg atgaaacatg gtcgggactg aagctcgtca tcccggtaaa agaccggaaa
     2761 aaattgacat aggtcaaccg gccgcctgtc gtgataagat ttatctttac atcaactttt
     2821 tatttacggt atgcgtcttc ggtttttacc ggttttcttt cttcccggcc tgttttttct
     2881 cgccgcgtgt tcgtccaacc cgggaccgaa aaaagacatc gtttcaaaat acatcggcgt
     2941 gctgccgacc gtcgtggata acgatgaggg tctttttcac gggattatgc tcggcatgac
     3001 gccggaagaa gtgaaagcgc acgcgatctc acgtgacagc ctcggtgaag aaaaacccgg
     3061 ctacctcatg ttccaggggc gcatttccgg gaaagaagaa tatctctacg actgccagtt
     3121 cgatacaaac gggctgttca cgctggagct cgacatttac ctcaaggaca aaccttccgg
     3181 cgattccatc ttcagtgatt tcaaaagcta ttacaccgcg cgtttcggta caccgcagga
     3241 agagaaaggc atgctcatct ggacgtgcaa cgaaggaacc cgcccggcga agatcatcat
     3301 cgatgaagat cccgcctatt cttacggcga gctgacggtc accatttacg acaaaacgtt
     3361 cgacacaccg ccccccggtg tatccgacag cctgaacgga aactattccg acagcctcga
     3421 tgggaaagac acgctcatcc ttccctgata tttttccggg gagtcccgga acctgctgta
     3481 tttgccgtgc gtctcctgat cgtgtatatt taattcggca aacccgtttt tcatgaaaca
     3541 attccgcacg cgcatcgcca tcgtggcgat ctttctactc attgtattgc cggccgcagc
     3601 gcagcatacg ttcttcacgc tgctgaacgg gtatacgtcg atgccgacat gcatacagca
     3661 aaccgcggac ggaaattatt tcgtcactgc gcacggaaac cagtatttcc tgatgaacga
     3721 tacaggttca ctggcctggg aaaaaaacct gtacgacccc aatctttatt ttgattgcca
     3781 gtataacctt cccacacccg atcatggttt tattttttcg ggtcctgtca ctgatcctgt
     3841 gctgggctcc tatggataca tcgcgaagct cgattcctcc gggcatgtac agtggatgcg
     3901 gatgacagga ctggccgatg acccgatggg atattccatg tgcagcattg cagcgtctcc
     3961 ggacagcggt tatgtttttg cggccggcgt caatgtaaca acggcagcat cacccaaaag
     4021 aggagcgctg gtgatgaagc tcggcgcgaa cgggaatacg gactggtcgc gggtgattgt
     4081 cccggacacc gcctaccagc cgtaccgtgt gcaatcgacg tgcataaagg cgctgcccgg
     4141 cggagattac atcgtggcgg gtctggccga tttcaccctc ctgctctgca ggctcgactc
     4201 tgccggaaat ctccgctggg ttaaaactta cctgcaggat tcgctggccg gccagactgt
     4261 ttacgttgat acggtctccg acggcggttt cgttgtcagt gcgaatctcg acctggcgca
     4321 gagcaaccag taccaggcgt gcctgctgaa aacggattca acgggaaacc cgcaatggtg
     4381 ccggctttac ggaacgcccg ggcagatcga aataaaatgc gtgcgcacgc tggccggggg
     4441 cggttatgca ttcatgggaa tggaaaatac ttctccctgg cagggcgcgg ctcttgtaaa
     4501 aaccgatccg tccggtaatc ctgtctgggt ttcgaaattc gattccgttt tcatctggca
     4561 aaccataaag tggtccaaat accagttcgt gcagacgtcc gatctcgggt ttgttttcgg
     4621 cggcctcgtg atcagccagc agaccggcca gacgtatccc tgcatgctca aaaccgatcc
     4681 gcagggaatg acggcgtgcc gtggtcacca ggtttccatc agcacgcaga cgttaccggt
     4741 ttccgtggat acgatcggct acgaagagcc aggggaatca ccatcgcctt tcagccggca
     4801 ggaacaggcg gatatcatca ccgattcgct catctgttcc ggcgtattca ccgtaaatac
     4861 cgaaggcatt gaagaagcca gcgcatcgcc cggcgtgctg ctttatccga acccggcctg
     4921 cgatctgctg caggtgacag caggcatgtc ctgcacgttc gaattataca attccgccgg
     4981 tcagctgttg cagaaaaaag cagccggacc gggaaccgtg cagttcgaca ttcatgcgta
     5041 cccgccggga ctgtatttcc tcgtcaccga taccggaaca ggacgcacgg caagcaaatt
     5101 catccgcgaa tagaaccgcc cgtttttaca cccggccgca gggaggctat cttcattgcc
     5161 ggaaccgccc gaatgcttat ttttaacctt ctatggaaag ctcaaaagcg gagacagaag
     5221 aaacaacccg tgtcaaccat ccgtcgggtg ttcttcttga agtgcggaat ctcgtgacgg
     5281 aattccgtac cgagaatgaa acggtgaaag ccgtcaacga tatttcattc agcatcaagg
     5341 ccggcgaaac gatcggcatc gtaggcgaat ccggttcggg aaaatcagtt acctctcttt
     5401 cggtgatgcg tcttattccg aatcctccgg gcgagatcac gggcggggaa gttcttttca
     5461 cctcgcgcaa cgacggtgtc atcgacctgc tgcagatgcc ggaaaagaaa atgcagaaat
     5521 accgcggcaa cgagatcgcg atgatcttcc aggagccgat gacctcgctc aacccggtgt
     5581 attcctgcgg tgagcaggtg atggaaacca ttctccagca ctggaaatcg ccgtccattc
     5641 ccgattcacg gaatttcatc gaaaaaacaa tccgcagctt atacaagttc atgctcgtct
     5701 tcggttcgat cttcatcggc tgcatgatgt tttacctgct tttctccgat aaatccttta
     5761 ccggcaaact gctgctgatc ctttcgacgc tctacttcac ggggctgttt actttttatt
     5821 tctttcgtaa aagagccggc gtttaccggc cgttccatta ttacacgtcg cgcgaacgcg
     5881 tcgcgaaaaa aaatacgctc gatcttttcg gccaggtaag gctgcccgat cccgaacgca
     5941 ttttctacag ctacccgcac cagatctcgg gcggacaaaa gcagcgcgtc atgatcgcca
     6001 ccgccatggc gtgcaacccg cagctgctca tcgccgatga gccgaccact gcgctcgacg
     6061 tcaccgtgca gaaaacgatc ctcgagctca tgcgcgatct gcaggcggaa cacaacatga
     6121 gcatcatgtt catcacgcac gacctcggcg tcattgcaga gctcgccgac cgcgtggtgg
     6181 tgatgtacaa aggcaagatc gtcgagcagg gttccgtact ggatattttt tcaaacccgc
     6241 agcacccgta cacgaaaagc cttctcgcct gccgcccgcc gctcaacaag cgcctgcagt
     6301 ggctgcctac cgtaagcgat ttcatgagcg tggacaatgc cggcgaaatg gtcgagaaca
     6361 gccgctccgt gctggaagtg acgcaccgcc tggaagtgac gaaggaagac cgcaaacgcc
     6421 agcacagtga gctgtacaac cgcgagccca tcctgaagct gaacaatatc cgcacacatt
     6481 tcgtgacgca gcgtaacgca ttcggaaaac cggtgcacgt ggtccgcgcg gtggatgaca
     6541 tttccttcga cgtttatccc ggcgaaacgc tcggtctcgt gggcgaatcc ggctgcggaa
     6601 aaaccacgct gggcagaacc atcctgcgcc tgatcgagcc gacaggcggg acgatcatct
     6661 acaagggaaa agacatcacg aaactttcgg cgcacgatat gcggttgctg cgcaaagaca
     6721 tccagttcat tttccaggat ccttattctt cgctcaaccc gcgcatcacg atcggcgacg
     6781 cgatcatgga accgatgaag gtgcacaaca tcggcaaatc ggaaaaagac cggaaggaac
     6841 gtgtgatcga gctgcttcac cgcgtgaaca tgaacgaaac gcatttctac cgttacccgc
     6901 atgaattttc cggcggacag cggcagcgca tctgcatcgc ccgcgcgctc gcgctgaacc
     6961 cgcagttcat catctgcgac gaatccgttt ccgcgctcga tgtttccgtg caggcgcagg
     7021 tgctcaacct gctcaacgag ctgaagaaag aattcaagtt cacctacatt ttcatctcgc
     7081 acgacctcag cgtcgtgaag ttcatgagcg accgcatggt ggtgatgaac gaaggaaaga
     7141 tcgaagagat gggtgaagcg gacatgatct acaacgatcc gcagaaagaa tacacgaaga
     7201 agctcatcaa cgcgatcccg aaaggcgaac tcgacgatat acgcgcttcc attgcccgca
     7261 aacgcatgcg cgcacaggcg ttctgatccg cttccgcgtt ccttccctgc ccgatgaaaa
     7321 agcgatggcc ttttcttttt ttcccggcga cgttcctgct gctgtggatc ctcgctttgc
     7381 tctcgaaagg catttacgac ccgggcgatg gcgtgatgca ttaccttttc gcgcattacg
     7441 cagggaaaca tcccgctctt tttctcgatc actgggcgaa gccgctctac acgttgcttt
     7501 cttttccctt cgcggttttc ggttacggcg ggacgcttgc gttcaacctg ctctgtctct
     7561 gcgcttcggg atggttcacg tggaagatcg cggaaaagct gaagctgccg ttcgcgccgc
     7621 tggccgcacc gctggtgatc ttcgcaccca tcgcattgcc ggtcggcatg agcgggctca
     7681 ccgagccgct tttcatgctc gtgcttgttg ccgggatctt ttttgtcatg aacggaagga
     7741 acggcattgc cgctttggtg atctcgctgc tgcccttcgc gcgcacggaa ggattttttc
     7801 tcgcgccgct tttcgcattc tattttctcg gccggaaaga ttttgttccc gttttcctgc
     7861 tgggcgcggg caccctcctc tattccgtgg caggcgcttt tctttaccac gatattctct
     7921 ggatcattca caaaaacccg tacacgggcg ctgaaaaaat ttacggccac gggaccctgt
     7981 tccacttcat cgcgctcaat gaattcacct ggggctgggg aatgaccgcg ctgctggcca
     8041 tgggaatgat cgccgcggtt ttccggaaac ggttcgccgc ttccgtttca tccgccgagg
     8101 gttggctcgt ctacggaacg ttccttcttt ttctcgtgat gcattccgtg ttctggtgga
     8161 aaggactgtt cggctcgctc ggacttcacc gcgtgatggc gtgcacgatc ccttccgccg
     8221 tgctgatcag cctgcgcggg tttcatgtga tcggggcgta cgtacgttcc gcggcggcga
     8281 tgaaaaccat tgcggggatc gtccttgcgg tgcaggtttt cctcacgtgg cgccagcatc
     8341 cgctcccgct gcagccttcg gatgattttg cgatcgatcg taccgcggcg gattttctcg
     8401 cggaaaacaa ttacaccggc gaacggatct tttcctcgca tcccgttttc gctttcctcg
     8461 ccgggaaaga cccgttcgac aaaacgcagt tcgtttcgtt cacctgttcc tgcctcgaca
     8521 cggatttcag gaaaggcgac atcgtcatct gggattcgca ttttacaaaa ttcgattacg
     8581 gcataccgat cgaagaactt tccaatcatc cgaagctgaa agagctcgcc cgtttcgggg
     8641 aaatgccggc cacgcgggag gaagcctccg gcaaaaacct cgtaggaatt ttccaggtga
     8701 aataaaactc tgcagagttg aaatcccggc catcaggcgc caggcaacga acgggatact
     8761 gctttcttta gccgcgaaga acacaacgtt cgtacacgaa gcgcaccaag aagaacggca
     8821 aacgggaaat tgctattgcg ggatcttccc gagatacacc cagtcgcctt tcagctttgt
     8881 gcaggtgtaa tctttcagcg ggatgtgact ggtcagctct ctttcatgct ggaaaaaatt
     8941 cacgtgctca tcgagcgtat agtaataata ccgctggcag ggcgtgacga taaaaacctg
     9001 tttgcgcgca atgcgcacga gctcctccat gcactggtcg agcttcagca ggtgctccac
     9061 cgtgtgactg cacgtaacga catcgaatga tttgtcgggg aaaggcatgc gctcgatatt
     9121 tcccttcaca taggaataca catccgcatt gtcgtcgctg tccttgatat cgaaaccggt
     9181 gagcttcagc tgcggatggc ggcggtgaat ttccttcagc aggaacccgt ttcccgatcc
     9241 cacatcgata agcgtctgcg ccgaaggatc gatgccgtcg agaataaaac gcaggctggg
     9301 cttgttgaga tccgtgacgc ggttgcgcga gatcgtatcg aggttgttgt agaaatgatc
     9361 gtaatcttcc ggcgtgaacg aatgcacgcg gcttttgaaa tccatcacct cgcggatatt
     9421 ttttccgcgg taggcaaaca tgtagaacgg gtacatgaac cagcggctgt cgcggatcac
     9481 cggcgggatc cattcatcca tgaaatagcg tatgacgttg gtgatgtctc
//
LOCUS       DASTSF010000605         2961 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_570787,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000605 DASTSF010000000
VERSION     DASTSF010000605.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2961)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2961
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_570787"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1483
                     /locus_tag="VFU15_17090"
     CDS             <1..1483
                     /locus_tag="VFU15_17090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017258210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEU4719564.1"
                     /translation="ALERRFQKVIVEPTTVDETIQILNNIKQKYEDHHNVTYTDDAIK
                     ACVTLTSRYITDRHLPDKAIDALDEAGSRVHITNIKVPKKILEVEAEIEKVKEEKNQV
                     VRSQRYEEAARLRDRERQLQEQLEAAKKQWEEESRSHRETVTEENVAEVVSMMTGIPV
                     QRVSQNESEKLAVMFDKLKGKVIGQDEAIAKVVKAIQRNRAGLKDPNKPIGSFVFLGP
                     TGVGKTQLAKILAKYLFDNEDSLIRIDMSEYMEKFAVSRLVGAPPGYVGYEEGGQLTE
                     KVRRKPYSVVLLDEIEKAHPDVFNLLLQVLDDGQLTDSLGRKVDFKNTIIIMTSNIGS
                     RQLKDFGTGVGFSTGAKEENINEHAKGVIENALKKAFAPEFLNRIDDVVMFNSLSRED
                     IYKIIDIELEKLYSRINQMGYSVQLTKEAKDFIVEKGFDANYGARPLKRAIQKYIEDP
                     LAEEIIRAGVSEGDTVTIGYDEEKKDIAIIVAKPKPKKNKKEE"
     gene            complement(1575..2246)
                     /locus_tag="VFU15_17095"
     CDS             complement(1575..2246)
                     /locus_tag="VFU15_17095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719565.1"
                     /translation="MRAWKFSLFIVFLLRFGGASAQIREGVNPFLMSIRGTATVPHPV
                     TNKAFRRSFTGIYDATVSCDYQVFPGAIIGAMYRNNLWKTPDNKIPGLNTYAQSNNFG
                     LRIGYDFPINETAVGFFGLTAEQGYYHFYGLSINEGTDLTELKTKYAYHALEGEGGVY
                     FYTEGNFAIGLNASYLFSNFKFNPYRLFLNQHKAYIASDLNGTVSQLNVGFCVVFSFL
                     KKKKK"
     gene            complement(2338..>2961)
                     /locus_tag="VFU15_17100"
     CDS             complement(2338..>2961)
                     /locus_tag="VFU15_17100"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEU4719566.1"
                     /translation="DCTGHGVPGALMSMIGFNFLGQIVNEMHIRKTSAILDELHRKIL
                     FALNRNLASGDVKDGMDIALLRIFKGSRKIEFSGAVRPLYIVNAGKLNIIDGDIYSIG
                     GIKDPEKESFSCHEMEVESGSMLYLFSDGYADQFGMSSEERGKVSAKTGTPAGKKFKY
                     RRLRELLSAVSGKPADEQQRLISETFLAWKGNLEQVDDILVIGIRPD"
BASE COUNT          795 a          732 c          801 g          633 t
ORIGIN      
        1 agcgctcgaa cgccgtttcc agaaagtgat cgttgaaccg accaccgttg acgaaacgat
       61 ccagatactg aacaacatca agcagaaata cgaggatcac cacaacgtca cgtatacgga
      121 cgacgcgatc aaagcgtgcg taacgctcac ttcgcggtac atcaccgacc gtcacctgcc
      181 ggataaagcg attgacgcgc tcgacgaggc gggttcacgc gtgcacatca cgaacataaa
      241 agttccgaag aagatactcg aggttgaagc agagatcgaa aaagtaaaag aggaaaagaa
      301 ccaggttgtc cgcagccagc gttacgaaga ggcggcgcgc ctgcgcgacc gtgagcgcca
      361 gctgcaggag cagctcgaag cggcgaaaaa gcagtgggaa gaagaaagcc gttcgcaccg
      421 cgaaacggtg acggaggaaa acgtggctga ggtcgtttcg atgatgaccg gcatccctgt
      481 acagcgtgtt tcgcagaatg aaagcgaaaa gctggcggtg atgttcgaca agctgaaagg
      541 aaaagtgatc gggcaggatg aagcgattgc gaaagtggtg aaagccatcc agcgtaaccg
      601 cgccgggctg aaagatccga acaagccgat cggttcgttc gtgttcctcg gtcctaccgg
      661 cgtcggaaaa acgcagctcg caaaaattct tgccaagtac ctgttcgaca atgaagattc
      721 gcttattcgc atcgacatga gcgaatacat ggagaaattt gcggtatccc gcctcgtggg
      781 agcgcctccg ggatatgtgg gctacgaaga aggcggacag cttactgaaa aagtgcgccg
      841 caagccgtat tcggtcgtcc tgctcgatga aatcgagaaa gcgcacccgg atgtattcaa
      901 cctcctgctg caggttcttg acgacggaca attgacggac agcctcgggc ggaaagtcga
      961 tttcaaaaat acgatcatca tcatgacgtc gaacatcggt tcacgccagc tgaaggattt
     1021 cggaacgggc gtaggttttt ccaccggcgc caaggaagag aacatcaacg agcatgcgaa
     1081 aggcgttatt gaaaatgcgc tgaagaaagc gttcgctcct gaattcctga accgtatcga
     1141 tgacgtggtg atgttcaatt cgctttcacg cgaagacatc tacaagatca tcgacatcga
     1201 gctcgaaaag ctttattccc gcatcaacca gatgggatac agcgttcagc ttaccaagga
     1261 ggcgaaagat ttcatcgtcg agaaagggtt cgatgcgaat tacggagcgc gtccgctgaa
     1321 acgggcgatc cagaaataca tcgaagatcc gctggcggaa gaaatcatcc gcgccggtgt
     1381 gagtgaaggc gacacggtga cgatcggcta tgatgaagag aaaaaggaca tcgctattat
     1441 cgtggcgaag cccaaaccga aaaagaataa gaaggaagaa taaccggaag ccccgcaagg
     1501 ggctttttta atgccgggaa ttccgggcgg gaaaattcat ggtacggatg accggctgaa
     1561 ctggtttgcc ggaattattt tttcttcttc ttgaggaaac tgaacacgac acagaagccg
     1621 acgttcagct gtgaaaccgt tccgttaaga tcgctggcga tgtacgcttt gtgctggtta
     1681 agaaaaagcc tgtagggatt gaatttgaaa ttggaaaaaa gataagacgc attcagcccg
     1741 atcgcgaaat tcccttctgt gtaaaaataa accccgccct ccccttccag cgcatggtag
     1801 gcgtattttg ttttcagttc agtgaggtcg gttccttcat tgatggaaag tccgtagaaa
     1861 tgataatagc cctgctctgc cgttaaacca aaaaagccga ccgccgtttc attgatagga
     1921 aaatcatagc caatacgcag cccgaaatta ttgctctgcg cgtatgtgtt cagcccggga
     1981 attttattgt cgggtgtttt ccagagattg ttcctgtaca ttgctccgat gatggcgccg
     2041 ggaaatacct ggtaatcgca ggaaaccgtg gcgtcgtaaa tgccggtgaa gctgcgccgg
     2101 aacgctttgt tcgtgacagg atggggaacc gtcgccgtgc cgcggatgct catcaggaac
     2161 gggtttacgc cctcgcgaat ctgtgcggaa gctccgccaa acctgagcag gaaaacaata
     2221 aaaagggaaa atttccaggc tctcataaac cggttgtcca aatataacgg gaaaaagtgc
     2281 aactgatcag gtactgatca gacgcagaaa ccgctgaacg gttgccggga aaagcattca
     2341 gtcaggccgg atgccgatga cgagaatatc atccacctgt tcgaggttcc ctttccatgc
     2401 aaggaacgtc tcgctgatca gccgctgctg ctcgtccgca ggttttccgc ttacggccga
     2461 cagcagctcg cgcaggcggc ggtatttgaa ttttttcccg gccggtgttc ctgtttttgc
     2521 agaaactttt ccccgttctt cgctgctcat gccgaactgg tcggcgtagc cgtcggaaaa
     2581 aagatagagc atgctcccgc tttccacttc catttcgtgg caggaaaacg attctttttc
     2641 cggatccttt atcccgccga tcgaataaat gtcgccgtcg ataatgttca gctttcccgc
     2701 gttcacaatg tacagcgggc gcacagctcc cgaaaattcg atcttccgcg agcctttgaa
     2761 aatgcgcagc agcgcaatgt ccattccatc ttttacatct cccgaagcaa ggttgcggtt
     2821 aagcgcaaaa agtattttgc ggtgaagctc gtcgagaata gcggacgttt tccggatgtg
     2881 catttcattg acgatctgcc cgaggaaatt gaaaccgatc atgctcatga gcgcgccggg
     2941 aacaccgtgc cccgtgcagt c
//
LOCUS       DASTSF010000606         2045 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_837554,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000606 DASTSF010000000
VERSION     DASTSF010000606.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2045
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_837554"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1168
                     /locus_tag="VFU15_17105"
     CDS             <1..1168
                     /locus_tag="VFU15_17105"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEU4719567.1"
                     /translation="ITMVNSGNNTNNRNFTLLGDPAVRLNYPMNKVVTSEINNVPVNL
                     SQPDTLRALSLVTVKGYVADTAGNQMTGFNGIIYPTVFDKPKTITTIGNDPPNPPFTF
                     QLQKSVIYHGKVSVVNGNFQFSFVVPKDIAYQFGNGRISYYAEDGQTDASGYFNSFII
                     GGSNPSPPADVTGPQVKLFMNDSNFVSGGITNSDPKLYAIIRDSSGVNTVGNGIGHDV
                     VAILDEATDNEIILNDYYTADLNSYQSGKILYSFSNLPEGHHTLSLKVWDVYDNSTTV
                     KTEFVVTNPAGLSIDHVLNYPNPFTTHTSFFLEYNQCCTDYDVMIQIFTVSGKLVKTI
                     NQHVAAEGFRSDPIDWDGRDDYGDLVGRGVYIYRVVAQSSDGKSAEKYEKLVIL"
     gene            1223..>2045
                     /gene="porV"
                     /locus_tag="VFU15_17110"
     CDS             1223..>2045
                     /gene="porV"
                     /locus_tag="VFU15_17110"
                     /inference="COORDINATES: protein motif:HMM:NF033710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system outer membrane channel
                     protein PorV"
                     /protein_id="HEU4719568.1"
                     /translation="MNNRAKRILTAAGFVSSVALAAQSPSAFYRSGPPVSTQSSSTAS
                     TQDVNDFLSHMNTITTAVPFLMITPDSRAGGMGETGVATTPDVNSIHWNGAKLAFADK
                     KMGLGISYTPWLRALVPDINLAYVSFYAKPDKNSAFGASLRYFSMGNITFTDVSGNTI
                     GQFRPNEFAFDFCYARKLGKFFSASMAGRIIRSNLTNGITVQGQDTKPGTSGAVDLSA
                     YFHDDEIKVSGKKSVLMAGIAITNIGSKISYSTSVNRDFIPINLRLGSGMQIEADE"
BASE COUNT          516 a          547 c          523 g          459 t
ORIGIN      
        1 gatcacgatg gtgaacagcg gcaacaacac gaacaacagg aactttacgc tgctcggaga
       61 tccggccgtg cggctgaatt acccgatgaa caaagtggtg acgtcggaga tcaacaacgt
      121 gccggtgaat ctttcacagc ccgatacatt gcgggcgctc agcctcgtca cggtaaaagg
      181 atatgtggcg gacacggcag gaaaccagat gacaggattt aacggcatca tttacccgac
      241 cgtattcgac aagccgaaaa cgatcacgac catcgggaat gatccgccga acccgccgtt
      301 cactttccag ctgcagaaaa gcgtgatcta tcatggaaag gtttctgttg tcaacggcaa
      361 tttccagttc tctttcgtcg tgccgaagga catcgcttac cagttcggca acgggcggat
      421 aagctattac gccgaggacg ggcaaaccga tgcgagcggt tatttcaaca gtttcattat
      481 cggaggatcg aatccatcgc cgccggcgga tgttacaggt ccgcaggtga agctgttcat
      541 gaacgacagc aatttcgtat cgggcggaat aacgaattcg gatccgaagc tttacgcgat
      601 catccgtgat tccagcgggg tgaacaccgt ggggaacggc atcgggcatg atgtcgtggc
      661 gatacttgac gaggcaacgg acaacgagat catcctgaac gattactata ctgccgacct
      721 gaacagctac cagagcggaa agatacttta ctctttctcg aacctgccgg aaggacatca
      781 cacgctcagc ctgaaagtat gggacgttta tgacaattcc acgactgtca aaacggagtt
      841 tgttgttacg aacccggccg gactttcgat cgatcacgtg ctgaattatc ccaacccgtt
      901 tacgacgcat acctcgttct tcctcgaata caaccaatgc tgcacggatt acgatgtgat
      961 gatccagatc tttaccgtgt cgggcaagct cgtgaaaacg atcaaccagc atgtagctgc
     1021 ggaaggattc cgttccgacc cgatcgactg ggacggccgt gatgattacg gtgatctcgt
     1081 cggaagaggc gtctacattt atcgcgttgt agcgcaaagc agcgacggaa aatcggcaga
     1141 gaaatacgaa aagctcgtca ttctgtgacg taaaaaagcg aaggttaatt tttttaaatt
     1201 tgctatcctt aaaacaatga gcatgaataa cagggcgaaa agaattctga ctgccgcagg
     1261 ctttgtttca tctgtggcgc ttgcagcgca gtcgccgtcg gcgttttacc gcagcggccc
     1321 gcccgtatcc acccaatcgt cttccacggc ttccacgcag gatgtgaacg atttcctgag
     1381 ccacatgaat accatcacta cggctgttcc ttttctcatg atcacacctg actcaagggc
     1441 aggcggcatg ggagagacag gcgtagcgac cacgccggat gtgaactcca ttcactggaa
     1501 cggtgccaag ctggctttcg ccgacaagaa gatgggactc gggatttcct atacgccgtg
     1561 gctgcgtgcc ctggttcccg acatcaacct cgcttacgtt tcattttacg caaagcccga
     1621 taagaattcc gcattcggcg cctcgctgcg ttatttttcc atgggcaaca tcaccttcac
     1681 agatgtgtcg ggaaatacca tcgggcagtt ccgcccgaat gaatttgcat tcgacttctg
     1741 ctatgcgcgc aagctgggca agttcttttc cgcttccatg gcgggacgca ttatccgttc
     1801 caacctgacg aacggcatca ccgttcaggg acaggatacg aaaccgggaa catccggtgc
     1861 cgtggatctc agcgcgtatt tccacgacga cgagataaaa gtctccggca agaaaagtgt
     1921 gctcatggcg ggtattgcca tcacgaacat cggctccaaa atctcttatt cgacttccgt
     1981 caaccgcgat ttcattccca tcaatcttcg cctcggcagc ggcatgcaga tcgaagcgga
     2041 tgaat
//
LOCUS       DASTSF010000607         3615 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1275588,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000607 DASTSF010000000
VERSION     DASTSF010000607.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3615
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1275588"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..293
                     /locus_tag="VFU15_17115"
     CDS             <1..293
                     /locus_tag="VFU15_17115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009493060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Phenylacetic acid catabolic protein"
                     /protein_id="HEU4719569.1"
                     /translation="TNDELRQKFIDKTVAQAEYLGLTIPDPDLKWNAERGHYDYGEID
                     WVEFNNVIKGNGPCNKQRLEHHVNVHNENAWVREAATAYAKKKASKAEAMVA"
     gene            331..546
                     /locus_tag="VFU15_17120"
     CDS             331..546
                     /locus_tag="VFU15_17120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719570.1"
                     /translation="MAKSRFDTSRKLATLVAKSRFDTSRKNRDTQRDYGVKNSLVATL
                     NVTVETKEKAALPRRKVNEKKQKRHST"
     gene            717..1013
                     /gene="paaB"
                     /locus_tag="VFU15_17125"
     CDS             717..1013
                     /gene="paaB"
                     /locus_tag="VFU15_17125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018128001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaB"
                     /protein_id="HEU4719571.1"
                     /translation="MNNSNDTQFPLWEVFVQSKSGTPYKHAGSLHAADKEMALMNARD
                     LYTRRNEGTAIWVVLSTAIVASSPEDLGPFFDPSNDKAYRHPTFYTIPSEVKHI"
     gene            1024..1788
                     /gene="paaC"
                     /locus_tag="VFU15_17130"
     CDS             1024..1788
                     /gene="paaC"
                     /locus_tag="VFU15_17130"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019620406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaC"
                     /protein_id="HEU4719572.1"
                     /translation="MSNDQSALLRFVLRLGDNSLILGHRLSEWCSNGPFLEEDIAMSN
                     IALDYLGQARILYTYAGKVEGKGHEEDFFAYERREHEFTNSLLTEMKTPDFAFAVARQ
                     LYYSTFAWLLATELQKSKDETLAGYAGKAIKEFTYHLRHANDWTLRLGDGTEESHTRM
                     QKAIDDLWMYTGDLFATVESDAELVKNGIIPDVAALKSKWNEMIADVLERATLKMPDA
                     NAWQMEGSRTGRHTEHLGYIIAELQYVTRAYPGNKW"
     gene            1973..2494
                     /gene="paaD"
                     /locus_tag="VFU15_17135"
     CDS             1973..2494
                     /gene="paaD"
                     /locus_tag="VFU15_17135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014202583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaD"
                     /protein_id="HEU4719573.1"
                     /translation="MKKNIQLNPLVYTGKNPELWAIANEVVDPEIPVLTVVDLGIVRD
                     IEKEGDSITVSITPTYSGCPAMDMIATDIKKIFQQKGFADVTVKTILSPAWTTDWMSD
                     DAKERLRQFGISPPEHTTADKNALLGKSKTVKCPRCGSMHTEMISNFGSTACKALWKC
                     LDCKEPFDYFKCI"
     gene            3031..>3615
                     /locus_tag="VFU15_17140"
     CDS             3031..>3615
                     /locus_tag="VFU15_17140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014202581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEU4719574.1"
                     /translation="MSTILSENKNAVAVIRLNRPDKFNSFNREMALALQSALDAAEAD
                     KTIRAIFLTGEGKAFCAGQDLSEAIDPSGPGIEKIVREHYNEIILRLRRIEKPVVCAV
                     NGVAAGAGANIALACDIIVAGTSAAFIQAFSKIGLIPDSGGTFILPKLIGFQRAAALM
                     MLGDKVPAAEALQMGMLYKVFDDAQLQDEAMKIAV"
BASE COUNT          969 a          930 c          977 g          739 t
ORIGIN      
        1 aaacgaacga cgagctgcgc cagaaattca ttgacaaaac cgtggcgcag gcggaatacc
       61 tcggtctcac gatccccgat cccgacctga aatggaatgc agaacgcggg cattacgact
      121 acggcgagat cgactgggtg gaattcaaca acgtgatcaa aggaaatggt ccgtgcaaca
      181 agcagcgcct cgagcaccat gtgaacgtgc ataatgaaaa tgcatgggtg cgcgaagcag
      241 cgaccgctta tgcgaagaaa aaagcttcta aagcggaagc gatggtggcg tagtcacgtt
      301 tcaatacgtc gcgaaaaaat cgcgacactc gtggcaaagt cacgtttcga tacgtcgcga
      361 aaactcgcga cactcgtggc aaagtcacgt ttcgatacgt cgcgaaaaaa tcgcgacact
      421 caacgtgact atggtgtaaa aaacagcctt gtcgcgacac tcaacgtgac tgttgaaaca
      481 aaagaaaaag cggccttacc gcgacgcaag gtgaacgaaa aaaaacaaaa acgacactca
      541 acgtgactgt tgaaacaaaa gaaaaagtga cgttgtcgcg gaacgggtaa ctttaaacgc
      601 gataaaaaaa attgcatagc cctgattgag cacgcgtcag cgtgggaaag caggaggaaa
      661 gaaaataaaa tgcgcaggtg gtgcgctcca aaaaataatc atcacgcctg atcactatga
      721 acaactcaaa cgacactcaa ttcccgcttt gggaagtatt cgtgcaatcg aaatccggaa
      781 cgccgtacaa gcatgcgggc agtcttcacg ccgccgacaa agagatggcg ctgatgaacg
      841 cacgcgacct gtacacgcgc cgcaacgaag gaaccgcgat atgggtggtg ctttccaccg
      901 ctatcgtcgc ctcgagcccg gaagatcttg gtccgttctt cgatccgtcg aacgacaaag
      961 cgtaccgcca cccgacgttc tacacgatcc cttcggaagt gaaacacatt taagcgaaat
     1021 tccatgagca acgaccagtc agcgctcctg cgcttcgtcc ttcgcctcgg cgacaacagc
     1081 ctcatcctcg gtcaccgcct ttcggaatgg tgcagcaacg gaccgttcct cgaggaagac
     1141 atcgccatgt cgaacatcgc gctcgattac ctcgggcagg cgcggatcct ttacacctac
     1201 gccgggaagg tggaaggcaa aggacatgaa gaagattttt tcgcctatga aagacgcgag
     1261 catgaattca ccaattccct gctcacggaa atgaaaacgc ctgatttcgc atttgcggtg
     1321 gcgaggcagc tgtattattc cacgttcgcg tggctgctgg ccacggagct gcagaaaagc
     1381 aaggacgaaa cgctggccgg ttatgccggg aaagcgatca aggaattcac ctatcacctt
     1441 cgtcacgcca acgactggac gcttcgtctc ggtgacggaa cggaagaaag ccacacacgc
     1501 atgcagaaag cgatcgacga cctgtggatg tacaccggcg atcttttcgc gaccgtggaa
     1561 agcgacgcgg agctggtgaa gaacgggatc attccggacg tcgctgcgct gaagagtaaa
     1621 tggaatgaaa tgatcgcgga cgtgcttgag cgcgccacat taaaaatgcc tgatgcgaac
     1681 gcatggcaga tggaaggcag ccggacggga cgacacaccg agcatctcgg ttatatcatc
     1741 gcggaactgc aatatgtgac gagggcgtac ccggggaata aatggtaacg gatttcggat
     1801 ttcgaatttc ggatttcgga tttgagatgt tggagtggaa aaagggaatg ggggtatacg
     1861 tggcctgctt gtgaacgaaa gattatctct tttgtgtctt cgtggcaaaa aaagctggtt
     1921 gcaaagacct ggcgctgcgc ggtgaaaatt caaaacggat cgctacagaa tcatgaaaaa
     1981 gaacattcag ctgaaccctt tggtgtatac ggggaaaaac ccggagctat gggcgatcgc
     2041 gaatgaagtg gtggatcctg aaatccctgt gctgacggtg gtcgatctcg gcattgtgcg
     2101 ggacattgaa aaagaaggcg attcgatcac tgtttccatc acacccacgt acagcggctg
     2161 cccggcgatg gacatgatcg cgacggacat caaaaaaata tttcagcaaa aaggatttgc
     2221 ggacgtaacc gtgaaaacca ttctttctcc cgcgtggaca accgactgga tgtcggacga
     2281 cgcgaaggaa cggttgaggc agttcggtat ttccccgccg gagcatacca ccgccgacaa
     2341 gaacgcgctg ctcggaaaat caaaaacggt gaaatgtccg cgctgcggat cgatgcacac
     2401 ggaaatgatc agcaacttcg gatcgacagc gtgcaaagcg ttgtggaaat gtctcgactg
     2461 taaagagccg ttcgattatt tcaagtgtat ctgaggaccg tttttttaat tgatctccgg
     2521 gaatcctgcg gaaaaccagc tatggcgctg ccgggaggtt agtcagaaaa gcttcctgac
     2581 ccgatggctt cgcggttcgg ggggtaatgc gtttcaaatt gaattacggt tattttttcc
     2641 ctgcaacttt ttttagaaaa aagttgcgca aaaaagcgcg cccggagctg attttccgga
     2701 attctacgct tcgaaaagcg cccgcaaccc aagccgttcc gctgcgcttc acttacgggg
     2761 ttgcttacgc acattcctcc actgcttttc tgcaacttga attctgcgga aaaccagcta
     2821 tggcgctgtc ctgtttttca ttcgttatat aaagtgaaaa ttccagaacc cgacaaccag
     2881 gcgggcgtca tggatgtgcg gccagaagag ttttccgacc gggaatttcg tcgctgcgcc
     2941 ggcagcttgt ctttaaaaaa ttcaaactga aacgctgccg gactttctca ctttccagct
     3001 cataagcaat cccactgtat atttgttccc atgagcacaa ttctctccga aaacaaaaac
     3061 gcggtggcgg tgatccgcct caaccgcccg gacaaattca acagcttcaa ccgcgagatg
     3121 gcgcttgcgc tgcagtcggc gctcgacgct gcggaagcgg ataaaacgat ccgcgcgatc
     3181 tttctcaccg gtgaaggaaa agccttctgt gcgggacagg atctttccga agcgatcgat
     3241 ccctcgggac ccggcattga aaagatcgtg cgcgagcatt acaacgagat cattctccgc
     3301 ctgcgccgca ttgaaaaacc ggtcgtgtgc gccgtgaatg gcgtggccgc cggcgccggt
     3361 gcgaacatcg cgctggcgtg cgacattatc gtcgcgggaa cttctgccgc attcatccag
     3421 gcgttttcga agataggcct gatccccgac agcggcggaa cgttcattct cccgaagctc
     3481 atcggtttcc agcgcgccgc tgcgctgatg atgctcggcg ataaagttcc cgctgcggaa
     3541 gcgctgcaga tgggaatgct gtacaaggtt ttcgatgacg cgcagctcca ggacgaagcc
     3601 atgaagatcg ccgtg
//
LOCUS       DASTSF010000608         2819 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_126144,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000608 DASTSF010000000
VERSION     DASTSF010000608.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2819
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126144"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1901)
                     /locus_tag="VFU15_17145"
     CDS             complement(<1..1901)
                     /locus_tag="VFU15_17145"
                     /inference="COORDINATES: protein motif:HMM:NF024827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU4719575.1"
                     /translation="MLLLSYCGNGNNEKKTASADSTPKLLYLNHADSVHYVGMETCRK
                     CHEDIYETFIHTGMGQSFDVASMQKSAAKFGKQSTIYDKYRDMWYHSYFADSNMYICE
                     FRLEGKDTVYKRTEKVDYIIGSGMHTNSHMYSVNGYVFQMPMTFYTQQGKWDLPPGFE
                     GGYNSRFSRQIGLECMSCHNSYPGFREGSENNFTKIPNGISCERCHGPGSLHVQRKMA
                     GDIVDTSKYIDYTIVNPAKLPPDLQFDVCQRCHLQGNAVLKPGKSFFDFRPGMKLSDF
                     LTVFMPKYEGAEDKFIMASHAERLKMSQCFIQTEARKSKGPVDTLHPYRNSLTCVTCH
                     NPHVSVRETADEHFNNACKGCHFTGKSTLTKCSDTQAHLDAAGNNCVSCHMPRSGAID
                     IPHVTVHDHFIRRPSNDTAETGKVKKFLGLFAVNEKNPPKPIIARAYLQQFEKFSHDP
                     SYLDSAAALLPQQTTLDIRNNFTDLVHLYFLKEDFMQVKRITDNAGRQYALDSILVRP
                     SFDNEDAWTAYRIGEAFYHLGDMPAAEKFYKKAVDLAPFHPDFRSKYALTLAVQQKKF
                     EAKTEYQHVLDQYPDYVPALTNLGYLWLEDGDDKKAESYYSQALALDPDDPQALLNMA
                     GLYIYRKDY"
     gene            complement(2042..2581)
                     /locus_tag="VFU15_17150"
     CDS             complement(2042..2581)
                     /locus_tag="VFU15_17150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004568633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU4719576.1"
                     /translation="MQQVQLLLGERVRLRALEPQDVDLLYKWENDTAIWKASNTQTPF
                     SRFVLEQYIAASHMDLHTNKQLRLMITSKDGKDVGAIDLFDFEPQHQRAGVGILIAER
                     EDRGKGFASEALNLLIAYSFQQLHLHQLYCNVTVDNQESVLLFQKHNFIITGIKKDWI
                     RVGDTFMDELLLQLVRKGH"
BASE COUNT          610 a          693 c          809 g          707 t
ORIGIN      
        1 ctgtaatctt tgcggtaaat atacaaaccc gccatgttca gcagcgcctg cggatcatcg
       61 ggatcgagcg cgagcgcctg gctgtaatag ctttccgcct ttttatcgtc gccgtcttcg
      121 agccagaggt agccgaggtt cgtcagcgcg ggaacgtaat cgggatattg atcgagcaca
      181 tgctggtact cggttttcgc ttcgaatttt ttctgctgca ccgcgagcgt aagtgcgtat
      241 ttgctccgga aatcgggatg gaaaggagca aggtcgacgg cttttttgta gaatttttct
      301 gccgccggca tgtcgccgag atggtagaac gcttcaccga tgcggtaagc cgtccacgca
      361 tcttcattgt cgaacgaagg ccgcacgagg atgctgtcga gcgcatattg tcttccggcg
      421 ttatccgtga tgcgtttcac ctgcatgaaa tcttctttca ggaaatagag gtggacgagg
      481 tcggtgaaat tgttccgtat atccagcgtg gtctgctgcg gaagaagggc cgccgcggaa
      541 tcgagataag aaggatcatg cgagaatttt tcaaactgct gcaggtaggc gcgcgcgatg
      601 atcggcttcg gcggattttt ttcatttacg gcgaacaggc cgaggaattt tttcactttc
      661 cccgtttcgg ccgtgtcgtt cgaaggacgg cggataaaat gatcgtgcac cgtcacgtgc
      721 ggaatatcga tcgcgccgga acgcggcatg tggcaggaaa cgcagttgtt gcccgcggca
      781 tcgagatgcg cctgggtgtc gctgcatttc gtgagcgtgc ttttgccggt gaaatggcag
      841 cctttgcatg cgttgttgaa atgctcgtcc gcggtctcgc gcacggacac atgcgggttg
      901 tgacacgtca cgcacgtgag cgaattccgg tacggatgca gcgtgtcgac cggccctttg
      961 gattttcgcg cttcggtctg gatgaagcac tggctcatct tcaggcgttc tgcgtgggaa
     1021 gccatgatga atttgtcttc cgcgccttcg tatttcggca tgaacacggt gagaaaatcc
     1081 gacagcttca tgccgggacg gaaatcgaag aacgattttc ccggcttgag caccgcgttt
     1141 ccctgcaggt ggcagcgctg gcacacgtcg aactgcaggt cgggcggaag cttcgcggga
     1201 ttgacgatcg tgtagtcaat gtatttcgac gtgtcgacga tgtcgccggc cattttgcgc
     1261 tgcacatgca acgaacccgg accatggcag cgttcgcagc tgatgccgtt ggggattttc
     1321 gtgaaattat tttccgagcc ttccctgaat ccgggatacg aattgtgaca gctcatgcat
     1381 tcgagaccga tctgccgtga aaaacgtgaa ttgtatccgc cttcaaaacc gggcggaagg
     1441 tcccattttc cctgctgcgt gtagaacgtc atcggcatct ggaaaacgta accgttcacc
     1501 gaatacatgt gcgaattcgt gtgcatgccc gagccgatga tgtaatccac tttttccgtc
     1561 cgtttgtaaa ccgtgtcttt tccttcgagg cggaattcgc agatgtacat gttggaatcc
     1621 gcgaaatagc tgtggtacca catatcgcgg tacttgtcgt agatcgtcga ctgcttcccg
     1681 aacttcgccg cgcttttctg catcgacgcc acgtcgaacg attgccccat gccggtgtga
     1741 atgaaggtct cgtaaatatc ttcatgacac ttgcggcagg tttccattcc tacataatgg
     1801 acggaatccg catggttgag ataaagaagc ttgggcgtgg aatcggcaga agccgttttt
     1861 ttttcgttgt tgccgttgcc gcaatacgaa agcaggagca tgagagcagc tatcggggcg
     1921 aaaaccaaaa atatccggaa gcgtttcatc ctgcgggaat acgaaggtaa ggattggttc
     1981 ggtttacgct ttcggcgttt ctgcctgcag ggcataaaaa caaaaccgga aaccgggaaa
     2041 ctcagtgccc tttgcgtacc agctgcagca gcagctcatc catgaacgta tcgcccacgc
     2101 gtatccagtc ttttttgatg ccggtgatga tgaaattatg cttctggaac agcagcacgc
     2161 tttcctggtt gtcgaccgtg acattgcagt acagctgatg gagatgcagt tgctggaaac
     2221 tgtaggcgat cagcaggttc agcgcttccg aagcgaagcc tttcccgcgg tcttcacgtt
     2281 cggcgatgag gatgcccacg cccgcacgct ggtgctgcgg ttcgaaatcg aacaggtcga
     2341 tcgcgccgac gtccttcccg tctttcgacg tgatcatcag ccggagctgc ttgttcgtgt
     2401 gcaggtccat gtgcgacgcc gcgatatact gctccagcac aaagcgggag aacggggtct
     2461 gtgtattgct tgctttccag atcgcggtat cgttctccca tttgtagaga agatccacat
     2521 cttgcggctc gagggcgcgg agccggacac gttcaccaag taaaagctga acctgctgca
     2581 taggattaaa agtagggaaa ggtacagagt ttcgttaaat ccgtggagtt gttcaggctg
     2641 aatgaaaata ttgccttgaa cggaatagtt gtttgtgaaa atcctgctgg taaattaacg
     2701 ctgttccggg taaaatagcg ggcagcagtg aaaaaagttt cgccttacag ttcaatggga
     2761 acgggaaatt tcggtgcggg cggaacgaaa gcgatcgatg tttcttttcg cggtcacgg
//
LOCUS       DASTSF010000609         2178 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1217168,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000609 DASTSF010000000
VERSION     DASTSF010000609.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2178
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1217168"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..84
                     /locus_tag="VFU15_17155"
     CDS             <1..84
                     /locus_tag="VFU15_17155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019251367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsfS/YbeB/iojap family protein"
                     /protein_id="HEU4719577.1"
                     /translation="VVHVFQRETRQFYNLESLWADAEEVDI"
     gene            202..>2178
                     /gene="ftsH"
                     /locus_tag="VFU15_17160"
     CDS             202..>2178
                     /gene="ftsH"
                     /locus_tag="VFU15_17160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEU4719578.1"
                     /translation="MNQDNEQDRRMRDRMPPRKPQMPKSPNGFFWIYIIIVAGLIGFL
                     VPNMFTDLPEIGYNDFTALINDHAVKKIYRVSNENNVLELVIDEKKANAPSVLALKTK
                     IKGWHGPTPADVPHLQLSSTWTDELGKRITQTETDAHYSPGEFVDIQEIKRSNISDHL
                     SWLLPIVVLVVLWVFFMRRMGGGTGSQIFNIGKSRATLFDKDTMVNTTFNDVAGLEEA
                     KVEIKEIVDFLKNPKKYTDLGAKIPKGALLVGPPGTGKTLLAKAVAGEAKVPFFSLSG
                     SDFVEMFVGVGASRVRDLFRQAKEKAPCIIFIDEIDAIGRARGRSISQGANDERENTL
                     NQLLTEMDGFGTNSGVIILAATNRADILDRALLRAGRFDRQIYVDLPDLNERKAIFQV
                     HLKPLKLDKTVDLDFLAKQTPGFSGADIANICNEAALIAARHDKKFVEKQDFLDAVDR
                     IIGGLEKKNKIISKEEKRTIAVHEAGHATVSWMLKHAAPLVKVTIVPRGRSLGAAWYL
                     PEERQLTTTEQFMDEMCATLGGRAAEDVTFGKISTGALSDLERVTKQAYAMVMYYGLN
                     EKVGNISFYDSTGNEMMLSKPYSEHTAEVIDEEVKRIIDAAYEKTKKILTENKEKLMQ
                     LADKLIEKEVIFKEDLETIFGKRPWDEEEHKIISE"
BASE COUNT          586 a          591 c          586 g          415 t
ORIGIN      
        1 gtggtccacg ttttccagcg tgaaacccgg cagttctaca acctggaaag cctctgggcc
       61 gacgcggaag aagtggacat ctgaaccgga ctgttaagaa ttacaaaaaa caagacggaa
      121 cgatcttttt gaacggattc tgaagaccca gccaaaatat taagacccta taaccgacct
      181 ttaggaaaag aaatgagtga tatgaaccag gacaatgaac aggatcgccg gatgcgcgac
      241 cgcatgcctc cccgcaagcc ccagatgccg aagtcgccga acggtttttt ctggatctat
      301 ataatcatcg ttgcaggatt gatcggcttt ctcgtgccga atatgttcac cgatctcccc
      361 gagatcggct acaatgattt caccgcgctg atcaacgatc atgccgtgaa aaaaatatac
      421 cgcgtcagca atgagaacaa cgtgctcgag ctggttatcg atgagaaaaa agccaacgcg
      481 ccttccgttc ttgcactcaa aaccaagatc aagggatggc acgggcccac accggccgac
      541 gttccgcatc ttcagctgag cagcacatgg acagacgagc taggcaaacg cattacacag
      601 accgaaaccg acgcacatta ttcgcccggt gaattcgtcg acatccagga gatcaagcgt
      661 tccaatattt ccgatcacct gagctggctg ctgccgatcg tggtgctcgt cgtgctctgg
      721 gtgttcttca tgcgccgcat gggcggcggg accgggagcc agatcttcaa catcgggaaa
      781 tcgcgtgcca cactgttcga taaggacaca atggtgaaca cgacgttcaa cgatgtcgcg
      841 ggcctcgaag aagcgaaggt cgagatcaag gagatcgtcg acttcctgaa aaatcccaag
      901 aaatacaccg atctcggtgc aaagatcccg aaaggcgcgc tgctcgtcgg tcccccggga
      961 accggcaaaa cgctcctcgc caaagccgtt gccggcgaag cgaaagttcc cttcttctcg
     1021 ctttccggtt ccgacttcgt ggaaatgttc gtgggcgttg gcgcgtcacg tgtgcgtgac
     1081 ctcttccgcc aggcgaagga aaaagcgccg tgcatcattt tcattgacga gatcgacgcg
     1141 atcgggcgtg cgcgaggcag gtccatttcg cagggtgcga acgacgaacg cgagaacacg
     1201 ctcaaccagc tgctcacgga aatggacggt ttcggaacga acagcggcgt catcattctt
     1261 gccgccacga accgggccga tatcctggac cgtgcgctgc tccgcgcagg acgtttcgac
     1321 cgccagatct atgtcgatct tcccgacctg aacgaaagga aagcgatctt ccaggtccac
     1381 ctgaaaccgc tgaagctgga caagaccgtc gacctcgatt tccttgccaa gcagacaccc
     1441 ggattttccg gcgccgacat cgcgaacatc tgtaacgaag ccgcgctcat cgctgcgcgt
     1501 catgataaaa aattcgtgga gaagcaggat tttctcgacg cggtggatcg catcatcggc
     1561 ggactcgaga agaagaacaa gatcatttca aaagaagaga agcgcaccat tgccgttcac
     1621 gaagcgggtc atgcaaccgt aagctggatg ctgaaacatg ctgcgccgct cgtgaaagtg
     1681 accatcgttc cccgcggacg ctcgctggga gccgcgtggt acctgccgga agaacgccag
     1741 ctcaccacga cggagcaatt catggatgaa atgtgcgcca cgctcggcgg aagagctgcg
     1801 gaagatgtga cgttcggaaa aatatccaca ggcgcgcttt ccgatcttga acgcgtgacg
     1861 aagcaggcgt atgccatggt catgtattac ggcctgaatg aaaaagtagg caacataagc
     1921 ttctacgatt cgacaggaaa cgagatgatg ctttcgaaac cttattcgga gcataccgcg
     1981 gaagtgatcg acgaagaagt gaaacgcatc atcgatgccg cttacgagaa aacgaaaaag
     2041 atcctgacgg aaaacaagga aaagctcatg cagctggccg acaagctgat cgagaaggaa
     2101 gtcattttca aggaagacct cgaaacgatc ttcggcaagc gtccctggga tgaagaggag
     2161 cacaagatca tttccgaa
//
LOCUS       DASTSF010000610        10492 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1017482,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000610 DASTSF010000000
VERSION     DASTSF010000610.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 10492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10492
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1017482"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            320..481
                     /locus_tag="VFU15_17165"
     CDS             320..481
                     /locus_tag="VFU15_17165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719579.1"
                     /translation="MEISINSEDLFRFAEDEFPEEPGKPVIDALLRFSRSFETRPSSM
                     TGRIESIAN"
     gene            626..2110
                     /locus_tag="VFU15_17170"
     CDS             626..2110
                     /locus_tag="VFU15_17170"
                     /inference="COORDINATES: protein motif:HMM:NF022525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2779 domain-containing protein"
                     /protein_id="HEU4719580.1"
                     /translation="MSHYLLSKTSYIKGLQCSKALFLYRYYPQLRDPFSPSKKAVFSR
                     GHEVGFLARDLFPGGKDATEGRSPKSKETVGRTTELMKAGESVIYEAAFVHNEVLVLV
                     DILVRDGEKWKAYEVKSSLRLSRAYYDDAALQYYVLSGAGLQLSGFFLVHLSSDYVRN
                     GEIALPELFTVVPVLDSVKKKFAHVAGSVLAQKQLLSSPQVPEVRIGGHCFSPYECDF
                     RGQCWKNVPKGNVFELAGINRAEQERLFHAGYVFPEMIPAEENIPALVKLHSLARRSE
                     KTIADREKLNEFLRDCTDEMLFLDIENFQPAVPKFEGTKPFQALPFAYSIHRKRGNET
                     RHSLFIAEHGKDPRREFLGSFLRDTEGDAVIFAYDASAEKTTLNLLKKQFPEFSQAID
                     QRLARIRDLMRPFSEGWYYAAAMNCSVSLKSVLPALVPGMNYENLRIMNGGHAMSVYE
                     KLGDEDLFGKAESISALEEYCAMDTMGMLKIFEVLEKEAQAQAE"
     gene            2154..4313
                     /locus_tag="VFU15_17175"
     CDS             2154..4313
                     /locus_tag="VFU15_17175"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEU4719581.1"
                     /translation="MKRAVFLFLLFLPCCLIASGDSLFHLVSLPEREPGTNSDCSVPV
                     TGPWKYHKGDDTAWSNPAYDDSGWITADPSLDAKHVAPGTFENKGWFRIRVDVDTQLC
                     GKDLGLLMTQSGASEIYLDGRLMQRFGKISADPAGEIRFDPHFLPVPCRFSRAGVHLI
                     AVRYFDAVAQSKNAGQAPGFELKIGRFQEAVIAQYVNSNVLTAIFIFYFAFFAALGFL
                     HFMLFLFYRAEKSNLYYSLFASGFSLVFIGLLVRQNLLDPDVQNSFAYYSNFLSDIYG
                     PSLIAMLYSFFYGRLPRIFWLWIAVYATDFVLNAVHHDIPLFGLFAYLLFGAESLRVI
                     IAAIIKKREGAWILGSGIITTVSFFSFFAIFSAFAHTSINFNQGGWAGFVIGILVILA
                     TLSIPISMSVYLARNFARTSRRLSDKLAEVEMLSQKTIEQEKEKQRILETQKETLELQ
                     VKERTAEITEQKKVIEEKNKDITDSINYAKRIQDAILPSKEIKYEHFPESFVLFRPKD
                     IVSGDFYWFAEKDGNRFIASADCTGHGVPGALMSMIGNNILNHIVNEKNIVSPDSVLD
                     ELHREVRVSLKQHEQTETKDGMDIALCVFRKDGRLEFAGAQRPLWIFRNNGFEEIKGD
                     KFSIGGLQAESERKFTLHAPAVAPGDCIYMFSDGFADQFGGVNGKKYMTKRFREFLFS
                     ITAKKMQEQESLLDEELERWKAGATQVDDVLVIGVRC"
     gene            complement(4322..5689)
                     /gene="dgt"
                     /locus_tag="VFU15_17180"
     CDS             complement(4322..5689)
                     /gene="dgt"
                     /locus_tag="VFU15_17180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009580500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dNTP triphosphohydrolase"
                     /protein_id="HEU4719582.1"
                     /translation="MQWQTLFSEARLGDKQYKRLISPDRTNFERDFDRVVFSSAFRRL
                     QDKTQVIPLPESDFVHSRLTHSLEVSCVARSLGKIAGKEIIGRYGLTDIHASDFGAIC
                     ATAALAHDIGNPPFGHSGESSISNYFLNGNGAQFHDAVGDENKWSDLTGFEGNANGFK
                     LLTNYNDSAGGNISLTFSSLAAFTKYPTRSNKLEEHTPLFRGKVSQKKYGYFQSEKEI
                     FKTIFDHLEIDALTDYAWRRHPLAFLVEAADDICYRIIDFEDGIRIGLIPFTEGETLL
                     KEVCGKDFDAGRYASITDQREKIAYLRAKSINTLINHVTQLFLDMEKEMLGGTFDTSL
                     VDAIDPAVLKPLKAILDLSVENVYRSRNVIQIEAAGFNVVGELLDIFITAVNDYHSFG
                     KELRKKRPFSDKVIRLFPQQFLHAGSDDLYLRILRVCEFVAGMTDTYAVSLYRRLKGI
                     ELPGK"
     gene            complement(5709..6068)
                     /locus_tag="VFU15_17185"
     CDS             complement(5709..6068)
                     /locus_tag="VFU15_17185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009582275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEU4719583.1"
                     /translation="MKFIVKILISTLAVLATSAILPESMVHVANVKTALLVAVVLAFL
                     NAVVKPALIIMTISVTVFTLGFFLLVINVIIIKLADYFIDGFAVHGFWSALIFSFVLT
                     IVNSVFESLTKDDKHYN"
     gene            6244..7065
                     /locus_tag="VFU15_17190"
     CDS             6244..7065
                     /locus_tag="VFU15_17190"
                     /inference="COORDINATES: protein motif:HMM:NF026121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4476 domain-containing protein"
                     /protein_id="HEU4719584.1"
                     /translation="MKTYVKLSIIAALAVLPAAVNAMSSEIRLSASDNSKFVLKFDNS
                     FFQTPSNTYEVTMIEPGMHHIVMRHATTTQYGACGMPSVVFDGWVNIPENEKIDAVAL
                     NEHLLRFNSMIPITQGYGYGGHGHDGGDYYGNNNGTNGGYNNGNGNYGTGNSGCGNSY
                     TNYGSNSGYGWNPQTYYAMSPNDFQVLKNTVSNQSFDSDKLIIAKQAVNMNHLSSQQA
                     YELVQLMTFESTKVELAKYAYGRTVDPGNYYLVNNAFTFSSSVSDLATYINNYHA"
     gene            complement(7273..8850)
                     /locus_tag="VFU15_17195"
     CDS             complement(7273..8850)
                     /locus_tag="VFU15_17195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014678403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEU4719585.1"
                     /translation="MIRSLRLSLTPAQAFDDTKLRYVICKEAGTGSEELSGWIIRKRS
                     IDARSRNVKIHLEIDAFIGEPVRSEKPQWTPRDVSAKPEVIVIGAGPAGLFAALKLIE
                     NGLKPVILERGKDVQKRRRDLAVLNKEHIVDPDSNYCFGEGGAGTYSDGKLYTRSHKR
                     GDVNRILNIFVAHGADETILADAHPHIGTNKLPKIITAIRENILRSGGEIHFETRVTD
                     LELDGNEITAVVARNGQAEKNFPAKKILLATGHSARDIFELLHAKKIRIEAKPFALGV
                     RVEHPQALIDSLQYHCRVDEVERAREFLPAAAYSLVHQAEGKGVYSFCMCPGGIIAPC
                     ATSQEEIVTNGWSPSKRNNPFANSGIVVSVEPAELKAYEKHGVFSALRFQQEMEHNAW
                     IAGGKTQTAPAQALADFVNGKLSAQLPECSYQPGVRSAPLHELLPAFIGKRLQEGFRV
                     FGQKMRGYLSNDALIVAVESRTSSPVRIPRDAEKFHHPQIVNLYPCGEGAGYAGGIVS
                     AAMDGEKCAEAIAGTMS"
     gene            complement(8847..9566)
                     /locus_tag="VFU15_17200"
     CDS             complement(8847..9566)
                     /locus_tag="VFU15_17200"
                     /inference="COORDINATES: protein motif:HMM:NF024957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HEU4719586.1"
                     /translation="MVRRIFLLFLPLLFTVQLWSQNQAQQNRPEYYTDPIHFGFYLGV
                     NRTNFQVTPVQNWRSVAGDSLKTILSSSSTGFNLGIVSELRMMEYACLRFVPAIAFGS
                     RNLDYHFEGRDTFDIQKRVESTFLDFPLDIKIRSKRLGNFAAYMTGGGKYTLDLASQK
                     DVNNQTATSAQQVVKLAKNDWCYEAGAGVDFFLPYFKFGIDLKFSMGLRNLLIKDNTV
                     FAQTLDSLHSRVFLISFTFEG"
     gene            complement(9595..10329)
                     /gene="ubiE"
                     /locus_tag="VFU15_17205"
     CDS             complement(9595..10329)
                     /gene="ubiE"
                     /locus_tag="VFU15_17205"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008242991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052624 - 2-phytyl-1,4-naphthoquinone
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0042372 - phylloquinone biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HEU4719587.1"
                     /translation="MSSTVTPYNSESGKKEQVAEMFDNIAHRYDFLNQLLSLGIHKGW
                     RKKAVRKLEEIHPLTILDVACGTGDFAIESFKRLQPEKVTGVDISEGMMKIGREKLEA
                     KKLSGKISLVYGDSENLPFAENSFDAATVGFGVRNFENLEKGLAGIFRVLKPGGKLVV
                     LEFSKPRKFPVKQLYRFYFLRVTPFIGKLFSKDSRAYSYLPESVNAFPDGEEFMRRME
                     KTGFSSVSWQPLTFGIASVYTGIKPQ"
BASE COUNT         2417 a         2699 c         2877 g         2499 t
ORIGIN      
        1 agacctgacc taacccttga gaggcttgtt cgggaataag acggagaaag ggtcgaaaag
       61 ttacccggtc gcgaaatatt ttcaggaata ttaacaaagc aggcgcagaa aggcatgtaa
      121 cttattcctt aagacacggt aaatgatttt taatatgtcc gtatcctgtt gtttatcagt
      181 atgttatttg cagtgtaatt gagaatcaaa tcctgttgtt ccaattttcg ccggaataaa
      241 acaataagca cccggaagct cccgttttac atgaaaacca aatctctccc gaatgagaaa
      301 aacttctact actgacgtaa tggaaatcag cataaacagc gaagatttgt ttcgctttgc
      361 tgaggatgaa ttccccgagg agcccggaaa acctgtcatt gatgcattgc tccgcttctc
      421 acgatctttt gaaacccgcc cgtcgtccat gaccggccgt atcgaaagca tcgccaattg
      481 aaaaaatggt tgtgatggtc ggagcccgcg tgatacatga tcatgcgggc ttcgtgtttt
      541 gcgaagcaat gcaggcctcc cggttcgcag gatagtcggt acctttgatc ttcacctcag
      601 tcactgcacc aggcacacga tcgttttgtc gcactattta ttaagcaaga cttcctatat
      661 caaaggactg caatgttcga aagcattgtt cctgtaccgc tattatccgc agctgcgcga
      721 tccgttctcc ccgtcgaaga aagcggtgtt cagccgcgga cacgaagtcg gtttcctggc
      781 gcgggacctt ttccccggcg gaaaggatgc gacggaagga agatcgccga aatcgaaaga
      841 aacggtcggg cgtacaaccg aactgatgaa ggcaggggag agcgttattt acgaagcggc
      901 attcgtccat aacgaggtgc tcgtgctcgt ggatatcctt gtccgcgacg gtgaaaaatg
      961 gaaagcctac gaagtgaaaa gttccctgcg gctttcgcgc gcgtattacg atgatgctgc
     1021 cctgcagtat tacgtgctgt cgggtgccgg ccttcagctg tccggttttt ttcttgtgca
     1081 cctcagcagc gattatgtcc ggaacggaga gatcgccttg cctgaactat tcactgttgt
     1141 cccggtgctc gattcagtga agaaaaaatt cgcgcacgtg gcgggcagcg tcctcgcgca
     1201 gaagcagctt ctttcttccc cgcaggtgcc ggaagtccgc atcgggggac attgtttctc
     1261 cccgtatgaa tgcgatttcc gcgggcagtg ctggaagaat gttccgaaag gaaatgtatt
     1321 cgagctcgcc ggcatcaacc gtgcggagca ggagcgtctt tttcacgcgg gatatgtttt
     1381 cccggaaatg atccccgcgg aagaaaatat tcccgcgctg gtgaagctgc attcgctggc
     1441 gcgccggtcg gaaaaaacaa ttgccgaccg ggaaaaactg aatgaatttc tccgcgattg
     1501 cacggacgaa atgctgtttc tcgacatcga gaatttccag ccggctgttc cgaagttcga
     1561 aggaacgaaa cctttccagg cattgccgtt cgcgtattca atccacagga aaaggggaaa
     1621 tgaaacccgg cacagtttat tcatcgcgga gcatggaaaa gatccgcgcc gtgaattcct
     1681 cggaagcttc ctgcgcgata cggaaggtga tgcggtcatt tttgcgtacg atgcatccgc
     1741 agaaaaaacc acgctcaacc tgctgaaaaa gcagttccct gaattttcac aggcgatcga
     1801 tcagcgcctg gcgcgcatcc gcgatctcat gcggccgttt tcggaaggct ggtattacgc
     1861 cgcggcgatg aactgctcgg tttcgctgaa gagcgtattg cccgcgcttg ttcccggaat
     1921 gaattacgaa aatctccgca tcatgaacgg cggtcacgct atgtcggtat atgaaaagct
     1981 gggtgacgaa gatcttttcg gaaaagcgga gagcatttct gcgctggaag aatattgtgc
     2041 gatggacacg atgggaatgc ttaaaatttt cgaagtgctg gaaaaagaag cgcaggcgca
     2101 ggcggaatga attttccttg gtttgaaggc agatagtaat aaatttgacg agcttgaaac
     2161 gcgcagtctt tttattctta ctctttttac catgctgcct catcgcttcg ggcgattcac
     2221 tttttcatct tgtctcgctc cctgaacgtg agcccggaac caattccgat tgcagtgtcc
     2281 ccgtaaccgg tccgtggaag taccacaagg gcgatgatac ggcctggagc aacccggcgt
     2341 acgacgacag cggatggatc acggccgatc cttcgctcga tgcgaaacat gttgcgcccg
     2401 gaacatttga gaacaagggc tggttccgga tccgggtcga tgtcgacacc cagctttgcg
     2461 gaaaagatct cggcctgctg atgacgcaat ccggcgcttc cgaaatttat ctcgacggcc
     2521 ggctgatgca gcggttcggc aagatctccg ccgatcctgc aggtgaaatc aggttcgatc
     2581 cgcattttct tccggttccc tgccgtttca gccgcgccgg tgttcacctc atcgccgtca
     2641 ggtatttcga cgcagtggcg caaagcaaaa atgcgggaca ggcccccggt ttcgagctga
     2701 agatcggccg cttccaggag gcggtgatcg cgcagtacgt gaattcgaat gtgcttacgg
     2761 cgatcttcat tttctatttt gcctttttcg ctgcgctcgg cttcctgcac ttcatgcttt
     2821 ttctttttta ccgggcggaa aaatcgaacc tgtattacag cctgttcgca tccgggttca
     2881 gcctggtttt catcgggctt cttgtaaggc agaatttact cgacccggat gtgcagaaca
     2941 gtttcgcgta ttactcgaat tttctttccg acatctacgg cccttcgctg atcgcgatgc
     3001 tgtattcttt tttttacggg agactgcccc gcattttctg gttgtggatc gctgtttatg
     3061 ccaccgattt tgtcctgaat gcagtgcatc atgatattcc attgttcgga cttttcgcct
     3121 acctgctctt cggtgcggaa tcactgcggg taattatagc ggcgatcatc aaaaaaagag
     3181 aaggcgcctg gatactcggt tcagggataa tcacgacggt ctcctttttt tcgttcttcg
     3241 ccatattttc cgcattcgcg cataccagca tcaatttcaa ccagggcggc tgggccggtt
     3301 tcgtgatcgg catcctcgtc attctcgcga cgctcagcat tcctatttcg atgtcggttt
     3361 acctcgcccg gaattttgca cgcacgagca ggcgcctctc cgataagctg gccgaagtgg
     3421 aaatgctttc gcagaaaacg atcgagcagg aaaaagaaaa gcagcgcatt ctcgaaacgc
     3481 agaaggagac gctggagctg caggtgaagg agcgtacggc cgagatcacc gagcagaaaa
     3541 aagtgatcga ggagaagaat aaggatatca ccgacagcat caactacgcg aaaaggatcc
     3601 aggacgctat tcttccgtcg aaagagatca aatacgaaca tttcccggaa tcattcgtgc
     3661 tgttccggcc gaaagatatc gtgagcggcg acttctactg gtttgctgaa aaagacggca
     3721 accgcttcat cgcttcggcg gattgcacag gacatggtgt tccgggcgcg ctcatgagca
     3781 tgatcgggaa caacatcctg aatcatatcg tcaacgaaaa aaatatcgtc tcgcctgatt
     3841 ccgttctcga tgagcttcac cgcgaagtgc gcgtttcgct caagcagcac gaacagacgg
     3901 aaacgaagga cggcatggac atcgcgcttt gtgttttccg caaagacggc aggctggagt
     3961 ttgcgggagc gcagcgcccc ctgtggatct tcaggaacaa tggattcgaa gagatcaaag
     4021 gcgacaaatt ttccatcggc ggactccagg cggaatcgga gcggaagttc acgctgcatg
     4081 ctcctgccgt tgcgcccggc gactgcatct acatgttctc ggacggtttt gccgaccagt
     4141 tcggcggtgt gaacgggaag aaatacatga cgaaacgatt ccgtgaattt cttttttcca
     4201 tcaccgcgaa aaagatgcag gagcaggaat ccctgctgga cgaagagctg gaacggtgga
     4261 aggcaggtgc aacgcaggtg gacgatgtgc tcgtcatcgg cgtgcggtgc tgatccctgt
     4321 tttatttccc gggcagctcg atccctttta atctcctgta caacgaaacg gcgtaagtgt
     4381 ccgtcatacc ggccacgaat tcgcagacgc gcagtatgcg caggtaaagg tcgtcgctgc
     4441 cggcgtggag gaactgctgc gggaagaggc ggatcacctt gtcgctgaac gggcgttttt
     4501 tccgcagttc ttttccgaac gaatgataat cattcaccgc ggtgatgaaa atgtcaagca
     4561 gctcgcccac gacgttgaat cctgctgctt cgatctgtat cacgttgcgg cttcggtaaa
     4621 cattttccac ggaaagatcg agtatcgctt tcagcggctt cagcactgcc ggatcgatgg
     4681 catcgacaag cgacgtgtcg aacgtgccgc cgagcatttc tttctccata tccaggaaaa
     4741 gctgcgtgac gtggttgatc agcgtgttga tggattttgc acgcaggtac gcgatctttt
     4801 cgcgctggtc ggtgatcgat gcgtaacggc ccgcgtcgaa atcttttccg cagacttcct
     4861 tcagcaaggt ttcaccttcg gtgaagggaa tgagcccgat gcgtatgccg tcctcgaaat
     4921 caatgatgcg gtagcagatg tcgtcggcgg cttccacgag aaaagcgagc gggtgcctcc
     4981 gccaggcgta atccgtcagc gcgtcgatct cgaggtgatc gaaaatggtt ttgaatattt
     5041 ctttttccga ctggaagtag ccgtattttt tctggctcac ttttccccgg aagagcggcg
     5101 tgtgctcttc cagcttgttc gaacgcgtcg ggtatttggt gaatgccgcc agcgaggaaa
     5161 aagtaagact gatgttcccg cccgcagaat cgttgtaatt cgtcagcagc ttaaaaccat
     5221 tggcatttcc ttcaaagccg gtgaggtcgc tccatttgtt ttcatcgccg accgcatcgt
     5281 ggaattgcgc gccgttcccg ttgaggaaat aattgctgat cgagctttcc ccgctgtggc
     5341 cgaacggagg gttgccgatg tcgtgcgcga gcgccgcggt tgcgcagatc gcaccgaagt
     5401 cggatgcgtg aatatccgtg aggccgtagc gcccgataat ttcctttcct gcgatcttcc
     5461 ccagtgaacg cgccacgcag gaaacttcga ggctgtgcgt cagccgggag tgaacgaaat
     5521 cgctttccgg caacgggatc acctgcgttt tatcctgcag ccgcctgaac gccgaggaaa
     5581 agaccacgcg gtcgaaatca cgttcgaaat tggtgcggtc gggcgaaatg agccgtttgt
     5641 attgtttatc tccgagtctt gcttccgaaa aaagtgtctg ccactgcata tcgttctgtt
     5701 aaggaaagct aattgtaatg cttgtcgtct ttggtcaggc tttcgaatac gctgttcacg
     5761 atcgtcagca caaagctgaa aattaatgcg gaccagaaac cgtgcacggc gaagccgtcg
     5821 atgaaataat ccgccagctt gatgatgata acgttgatga ccagcaggaa aaacccgagc
     5881 gtgaaaacgg tgacggagat cgtcatgatg atgagcgcag gtttcaccac cgcattcagg
     5941 aaggcaagga caaccgccac gagcagcgcg gtctttacat ttgccacgtg caccatcgat
     6001 tccggaagga tcgcggaagt ggcgaggacc gccagcgtcg agatgaggat cttcacgatg
     6061 aatttcatgc cgctaaagta gaacatagtt cttagttcgc ggtttccgca aaccataatt
     6121 aaactcacac ggctcttccc aaaaactgta aacaggaaac cggctgccgc ccggcgggaa
     6181 gaagtttggc acgcaacttg aaatgtcatc tgcagggaac ttcccggaaa ggagaaacaa
     6241 cctatgaaaa cctatgtcaa actatccatc atcgctgcgc ttgcagtatt gccggccgca
     6301 gtgaacgcga tgagctctga gatcaggctc tccgcatcgg acaattccaa gttcgtgctc
     6361 aaattcgaca acagtttctt ccagacgcct tccaatacgt acgaagtgac gatgatcgaa
     6421 cccggcatgc atcatatcgt catgcgtcat gccacgacaa cgcaatacgg tgcctgcgga
     6481 atgccttcgg tggtttttga cggctgggtg aacattccgg agaatgaaaa gatcgatgcg
     6541 gttgcgctga acgaacacct gctccgtttc aacagcatga tcccgatcac gcagggatac
     6601 ggttacggcg gacatggtca cgatggcggc gattattacg ggaacaacaa cggcaccaat
     6661 ggcggttaca ataatggcaa tggaaactat ggaaccggga acagcggttg cggcaacagt
     6721 tatacgaatt acggaagtaa ttccggttat ggatggaacc cgcagacgta ctacgcaatg
     6781 tcgccgaatg atttccaggt gctgaagaat actgtgagca accagtcgtt cgacagcgac
     6841 aagctgatta tcgcgaagca ggcggtgaac atgaatcatc tttcttcgca gcaggcgtac
     6901 gagctcgtgc agctgatgac attcgaaagc acgaaggtgg agcttgccaa atacgcctac
     6961 ggccgtacag tcgatcccgg gaactactac ctcgtcaaca atgcgttcac gttttcgtct
     7021 tctgtcagcg atcttgcaac gtacatcaat aattaccacg cataaaatct taccgcgcag
     7081 ttaccgcctg cgcgatctcg gaaccgggtt ttagtagcgt ttttcctggt ccggccaacg
     7141 ggggatctga caagttcagg tccccttttt tattttgaag tgtccgggct gctcctggaa
     7201 agaagtgaag ccgaaaaaaa tcagagtgaa tcccgtgcgt ggaaatattt tggcgcagtt
     7261 gcccgcctta gttcaggaca tcgtccctgc gatcgcttcc gcacattttt ccccgtccat
     7321 ggccgccgaa acaattccgc ccgcatagcc cgcaccttct ccgcagggat aaagattgac
     7381 gatctgcggg tgatggaatt tttccgcatc gcgcggaatg cgcaccggcg aggaagtcct
     7441 cgattccacc gcgacgatca gcgcgtcgtt ggaaagatag ccgcgcatct tctgtccgaa
     7501 aacacggaat ccttcctgca atcgtttccc gatgaaagcg gggagcagct cgtgcagcgg
     7561 agctgaccgg acgccgggct ggtaagaaca ttcgggcagt tgcgcagaaa gttttccgtt
     7621 cacgaaatcg gcgagggcct gcgctggtgc ggtttgcgtt tttcctcccg cgatccacgc
     7681 gttgtgctcc atttcctgct ggaaacgcag cgcggagaaa acgccgtgct tttcgtatgc
     7741 tttcagttcg gcgggttcaa cagagaccac gatgccggaa ttggcgaacg ggttgttgcg
     7801 cttcgagggc gaccatccgt tcgtgactat ttcttcctgg gaggtggcgc acggcgcgat
     7861 gatcccgccg ggacacatgc agaacgaata aacgcctttg ccttccgcct ggtggacgag
     7921 actgtacgca gcggccggaa gaaattcgcg cgcccgttcg acttcatcaa cccggcaatg
     7981 gtattgaagg gaatcgatca gcgcctgcgg atgctccacg cgaacgccca gcgcaaacgg
     8041 tttcgcttcg atgcggattt tttttgcgtg cagcagctcg aaaatgtcgc gcgcgctgtg
     8101 gccggtggcg agaagtattt tcttcgccgg gaaatttttt tccgcttgcc cgtttcgtgc
     8161 gacaacggct gtgatctcgt ttccgtccag ctccaggtct gttacgcgtg tttcgaaatg
     8221 gatctccccg ccggaacgca ggatattttc acgaatcgcg gtaatgatct tcggcagttt
     8281 gttcgtcccg atatgcgggt gcgcatcggc gagaatggtt tcgtctgctc catgcgcaac
     8341 gaatatgttc aggatgcggt tcacatcgcc gcgtttgtgc gaacgtgtgt acagctttcc
     8401 gtcggaataa gttcctgcgc cgccttcgcc gaagcaatag ttcgaatcgg gatcgacgat
     8461 atgctctttg ttgagaacgg cgaggtcgcg tctccgtttc tgcacgtctt ttccgcgctc
     8521 gaggatgacg ggcttcagcc cgttttcgat cagcttcagc gcggcgaaaa gtcctgcggg
     8581 tccggcgccg atgacgatca cctccggttt tgcgctgacg tcacgcggtg tccactgcgg
     8641 tttttcggaa cgcacgggct cgccgatgaa cgcgtcgatc tcgagatgga tctttacgtt
     8701 gcgcgaacgc gcatcgatgg aacgtttgcg tatgatccag ccgctgagct cttcgctgcc
     8761 cgttcccgct tctttgcaga tcacgtagcg cagctttgtg tcgtcgaagg cctgcgcagg
     8821 tgtgagcgat aaacggagtg agcggatcat ccttcgaacg tgaaggaaat aaggaacacg
     8881 cgcgagtgaa gggaatcgag cgtctgcgcg aaaaccgtgt tgtctttgat cagcaggtta
     8941 cgcagtccca tggaaaattt caggtcgatg ccgaacttga aatagggaag aaagaaatcg
     9001 acgccggcgc ccgcttcgta gcaccagtcg ttcttggcga gcttcaccac ctgctgcgcg
     9061 gaagtggctg tctggttgtt cacgtctttc tgcgaggcaa ggtcgagcgt gtattttcct
     9121 ccgccggtca tgtacgcggc aaaattgccg agacgtttcg agcggatctt gatgtcgagt
     9181 ggaaaatcga ggaacgtgga ttccacgcgt ttctggatat cgaacgtgtc gcgtccttcg
     9241 aaatgataat cgaggttgcg cgatccgaaa gcgatggcgg gaacgaaacg caggcaggcg
     9301 tattccatca tgcgcaattc ggaaacaatg ccgaggttga acccggttga cgaggaggaa
     9361 aggatcgttt tcagcgaatc gccggcaacg gatctccagt tctgcacggg cgtgacctgg
     9421 aaattcgtcc ggttgacacc gaggtaaaat ccgaaatgga tcgggtcggt ataatattcc
     9481 ggcctgttct gctgcgcctg gttctgcgac cagagctgca cggtgaaaag caggggcagg
     9541 aaaagcagga aaatacgtct gaccatatct taagtaaacg ggtgtaaaga tagattattg
     9601 cggcttgatg cctgtataga ctgaggcgat gccgaaggtc aaaggttgcc agctgaccga
     9661 cgaaaacccg gttttttcca tcctgcgcat gaattcttcg ccgtcgggaa aggcgttgac
     9721 ggattcgggg aggtaggaat aggcgcggct gtctttcgaa aacagtttcc cgataaaagg
     9781 cgttacgcgg agaaaataga aacggtacag ttgcttcacg ggaaatttcc gtggttttga
     9841 aaattcgagc acgacgagct ttccgccggg cttcagcaca cggaatattc ccgcgagtcc
     9901 tttttcgagg ttttcaaaat tccgtacacc gaatccgacc gtcgcggcgt cgaatgaatt
     9961 ttcagcgaag ggaagatttt ccgaatcacc gtaaacaaga ctgatcttcc ccgaaagctt
    10021 tttcgcttcc agcttttcac ggccgatctt catcatacct tcggaaatat ccacgcctgt
    10081 tactttttcc ggttgcaggc gcttgaacga ttcgatcgca aaatcgccgg tgccgcacgc
    10141 tacgtcgaga atggtcaggg gatggatctc ttccagcttc cgcactgctt ttttccgcca
    10201 gcctttgtga atgccgagcg aaagcagctg gttgaggaaa tcataacggt gcgcgatgtt
    10261 gtcgaacatc tccgccacct gctctttttt ccctgattcc gaattatacg gcgtaaccgt
    10321 tgagctcatc cgtcaaaggt aagaacaata agcgcgggaa ttttttacag ccggaggcgt
    10381 ttcgacagga gctgtgtggg cctttcgacg aggaaccaca ggatggtcga catgccgagc
    10441 gtgacgccga gcgcgacgaa cacaagcagg taggcgcccc aaggtccctg ct
//
LOCUS       DASTSF010000611         2830 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1915599,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000611 DASTSF010000000
VERSION     DASTSF010000611.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2830
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1915599"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            220..453
                     /locus_tag="VFU15_17210"
     CDS             220..453
                     /locus_tag="VFU15_17210"
                     /inference="COORDINATES: protein motif:HMM:NF027444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4834 family protein"
                     /protein_id="HEU4719588.1"
                     /translation="MQEVFFTILAIWLVIRLFNAFGSESRSRSSFRQQTHYHFHTSQD
                     RKKEGDVKVETKKQNQSRIPNNEGEYVDYEDIS"
     gene            801..>2830
                     /locus_tag="VFU15_17215"
     CDS             801..>2830
                     /locus_tag="VFU15_17215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719589.1"
                     /translation="MNNSFSDFMKRHAIKFVAVLVFAVVTVAFFSPVFFDGKRVRQGD
                     VTNFQGTAKEIMDYRAKTGKEPLWTNSMFGGMPAYQISTLYPNNLVQYVQDFFMKVIP
                     SPATAIFLCMVGFYFLLITLKVDPLAAMAGAFAFGFSTYFVIIIQAGHNTQAFAVAYM
                     APVLMGVFLTMRGKLFLGSALTALALALEINANHLQITYYLMLTVLLLGIGEAVRLYR
                     EGEVKYLVRAAGLLAVAAFVAVLPNITNLYLTNEYGKESTRGQSDITVDNTQQKKTSG
                     LPIEYATQWSYGKGETMTLMIPNFYGGASEAIQNYDKDALDDVDQNFRENIGSNSAYF
                     GDQPFTSGPVYVGAIICFLFVLGLFIVKDSIKWWLLGATALSILLAWGKNFIGFTEFF
                     FNYFPGYNKFRAVSMTLVIAELTMPILAMLTIREIMRDPGIIKAKMKKVLLALGLTGG
                     LSLLVWLAPSMFVTTVTDEETAQITQGIKGQGGSDAVIGQYIDELSNARTSIVKGDAI
                     RSFAFIALSFGLIYFYSRQKLSGNVFAIAMAGLVVLDMFLVAHRYLNSSNYEKARKNE
                     TPYVMSKADEMIISDQAQNHDLGSRVLNVAVQTWQDGSTSYFHKSIGGYHGAKLKRMQ
                     ELYEQVMENNVNQVRAAIQQGQNDSMLRIVLSQQPALNMMNAKYIIYNPDGGV"
BASE COUNT          654 a          773 c          708 g          695 t
ORIGIN      
        1 atcttcccag ttgtaacgca cattatcctc gctttcgttt ccttcgtggt gccgttccgc
       61 gaactcctcg tcgaaatcgg gatttatttc cctgatggtg actgtgactt tcattcagaa
      121 tatttgttaa cgtgtttcaa aaacgcacac taaattatcg cttatcacga ctggatgaac
      181 aggaggcttc gctttaaaat gttgtaactt tgtatcgcca tgcaggaagt atttttcacc
      241 atcctcgcca tctggctggt tatccggctc ttcaacgcgt tcggttccga atcgcgcagc
      301 cgctcatctt tccgtcagca gacgcattat cattttcaca cgagccagga ccgtaaaaaa
      361 gaaggtgacg tgaaagtgga aacgaagaag cagaaccagt cgcgtatccc gaacaacgaa
      421 ggcgaatacg tcgattacga agacatcagc tgatcgccgc gcagcatttc ccacttatcc
      481 gcagcatttc cccgattacc aaaaaagcct gccacttatt taaagcaggt tggaatgaac
      541 ttttggtgaa tgtatgttta cagtacaatt ctggttaagg tgtttggcag tccttttccg
      601 acctcccttg gggagcctcc accggagaac ggactgaaca aaaaaagaat cccccgcagt
      661 ttcccaggct aagcggggga ttcgcttttt acgctttttc acaccggcgc ttttccttta
      721 ggaatcatta actccgccct cttttgcctg acggtgattt tgataacttt gccctgcctt
      781 aattcctgaa aacaagcgct atgaacaatt ccttctccga tttcatgaaa cgtcacgcca
      841 tcaagtttgt ggcggtactc gtttttgcgg tcgtcaccgt ggcttttttc tcaccggttt
      901 tcttcgacgg aaaacgggtg cgccagggcg acgtcaccaa tttccaggga acggcgaagg
      961 agatcatgga ttaccgcgcg aagaccggga aagaaccgct ctggacgaac agcatgttcg
     1021 gcgggatgcc ggcgtaccag atatccacgc tttacccgaa caatcttgta caatacgtgc
     1081 aggatttttt catgaaggtc attccctcgc cggccaccgc gattttcctc tgcatggtcg
     1141 gtttctattt cctgctcatc acgcttaaag tcgatccgct cgccgcaatg gcaggcgcat
     1201 tcgcgttcgg cttctccact tattttgtca ttatcatcca ggcgggacac aacacgcagg
     1261 cgttcgccgt tgcatatatg gcgcccgtgc tgatgggcgt gttcctcacc atgcgcggga
     1321 aactctttct cggttctgcg ctgacggcgc tggcgctggc cctcgagatc aacgcgaacc
     1381 acctgcagat cacctattac ctcatgctca ccgttctcct gctcggcatc ggtgaagccg
     1441 tgcgtcttta ccgggaaggc gaagtgaagt acctcgtcag ggcggcgggc ctgctggccg
     1501 tggctgcctt cgtcgctgtc ctgcccaaca taacgaacct ctacctgacc aacgaatacg
     1561 gcaaagaatc cacgcgcggc cagtccgata ttaccgtcga caacacgcag cagaaaaaga
     1621 cttccgggct tcccatcgaa tacgctacgc aatggagtta cgggaaagga gagacgatga
     1681 cgctcatgat cccgaacttt tacggcggtg cgtcggaagc gatccagaat tatgacaagg
     1741 atgcgctcga cgacgtcgat cagaatttcc gggaaaacat cggcagcaac tctgcgtatt
     1801 tcggcgacca gccttttacc agcggaccgg tatacgtcgg tgcgatcatc tgtttcctgt
     1861 tcgtgctcgg actttttatt gtaaaagatt ccatcaaatg gtggctgctc ggtgcaaccg
     1921 cgctgtccat cctgctcgcc tggggaaaga atttcatcgg cttcacggaa ttctttttca
     1981 attatttccc gggctataat aagttccgcg ccgtttccat gacgctcgtg atcgcagagc
     2041 tgacgatgcc cattcttgcg atgctcacga tccgcgagat catgcgggat cccggcatca
     2101 tcaaggcgaa aatgaagaaa gtgcttcttg cgctcggact caccggcggc ctttcgcttc
     2161 ttgtgtggct ggctccctcg atgttcgtga cgacggttac cgacgaggaa accgcgcaga
     2221 tcacgcaggg cataaaaggg cagggtggaa gcgatgccgt tatcgggcaa tacatagacg
     2281 agctgtccaa tgcgcgtacg tccatcgtga aaggtgatgc catacggagc ttcgcgttca
     2341 ttgcgctttc attcgggctg atctatttct attcgcggca aaaattatcc ggaaatgttt
     2401 tcgccattgc aatggcagga ctcgtggtgc tcgatatgtt ccttgtggcg caccgttacc
     2461 tgaacagctc taactacgag aaagcgagga agaatgaaac gccgtacgtc atgtcgaaag
     2521 ccgatgaaat gatcatcagc gaccaggcgc agaaccatga tctcggttcc cgcgtgctca
     2581 acgtggcggt gcagacctgg caggacggaa gcacttccta tttccataaa tccatcggcg
     2641 gttaccacgg tgcgaagctc aagcgcatgc aggaattgta cgagcaggta atggaaaaca
     2701 acgtgaacca ggtgcgcgcc gcgatccagc agggacagaa tgactccatg ctccgcattg
     2761 ttctttcgca gcagcccgcg ctgaacatga tgaacgcgaa atacatcatc tacaatcccg
     2821 acggaggagt
//
LOCUS       DASTSF010000612         5026 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_736391,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000612 DASTSF010000000
VERSION     DASTSF010000612.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5026
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_736391"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..429
                     /locus_tag="VFU15_17220"
     CDS             <1..429
                     /locus_tag="VFU15_17220"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU4719590.1"
                     /translation="DKPSRWMLSDAMNAGLTSYLLRDCGKEEIIEALITTSKGEQFFC
                     GKIVNDVLKTGDDISMETEIASCDGLKISGREAEIIRLVAEGLTNKEIADKLFLSTHT
                     VTTHRKNIMAKTGVNNTAGLVLFAIRNNIITPNHFLFNTN"
     gene            556..1770
                     /locus_tag="VFU15_17225"
     CDS             556..1770
                     /locus_tag="VFU15_17225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719591.1"
                     /translation="MRHIISAAVFSLAISPVYAQVLTHEDSLSAGLVRSNSSTVISGY
                     GEARVTYDLIDRTGNADLTRNVLFVGHRFNQKIFMFSEWEIEDAKVEGGEPGGEIAME
                     QLFLKFNVNRDLYVNAGLFIPRLGIINENHLPTTFNGIDRPYVETFVIPSTWREMGIA
                     LYGQTPRLPGFNWTVGLFNGLNSEEFGNGSGIREGRFEGKNASASNLAVSAALLYYRD
                     AFRFQVSGYYGGTAGLSHRDADSLRLDSGPFGTPVALAEADAQYISEGFTFKALGTIV
                     AIPDAQNINRAYANNTPQMMWGAYAEAGYNVLHPFATPEKNLVVFARYEMLDLNAKMS
                     ENGIRDESLRQQYLVTGVTWMPVRGIAVKADYTMRTTGKPNPALQINPYVNGQVFYAT
                     RGFVNLGIAYSF"
     gene            1773..2201
                     /locus_tag="VFU15_17230"
     CDS             1773..2201
                     /locus_tag="VFU15_17230"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HEU4719592.1"
                     /translation="MQTRRQFLKAGCGLCLALPGIGIAASLLQGCASVPVVKASPSEN
                     KISVAVSSFGKSNYVLVRSAKLDNDILLVKKSDGTFTALLMQCTHQEQPLTVSGNTIN
                     CSAHGSMFDLDGNVTHAPATRALTKYIAAQEGEQVIIHLS"
     gene            2212..3279
                     /locus_tag="VFU15_17235"
     CDS             2212..3279
                     /locus_tag="VFU15_17235"
                     /inference="COORDINATES: protein motif:HMM:NF020932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imelysin family protein"
                     /protein_id="HEU4719593.1"
                     /translation="MKKNILFLFCTTVIFLSCRKHHDDDPQPATSTLTADVLSDLSDH
                     VIGATYNDLEAQASVLYQDVLTLQAATNETNLSACRQSWRECRSAWEQSEGFLFGPVE
                     SQNIDPRIDTWPVNFNDLDSVLSSSAVFTSGYIDSLDDALKGFHPVEYLLFGVNGNKA
                     ATDFTPRQLDLLTALTENLRSLTASIAAQWNAANPNGYRSQFVSAGSGSTVYTTQRSA
                     FEEMVNAMSGICDEVANEKIDGPYVAQDPSLEESPFAFNSTTDFTNNIKSVRNIYLGS
                     YTSDGKGIEDLVRQYDLSLDGSIKQKINAALAALGTVTVPFGQAIAQQPVQLQNSMSA
                     ISDLQQTIDNQLMPFIQAHTN"
     gene            3282..4667
                     /locus_tag="VFU15_17240"
     CDS             3282..4667
                     /locus_tag="VFU15_17240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HEU4719594.1"
                     /translation="MKRKFILASLTVSVVAFTIADGCRKPDIFDDADGNEAYSGGSQT
                     VFDNGSGAFGHSFPDMSSDKTELHATGDIAFSAPFVTAPAPLHSGLGPVYNNVSCSNC
                     HVADGRGKPPEANEQLLSMLFRISIPGTDEHGGPNPVPGFGGQLQQRAIAGKQPEADV
                     AISYTYTTGQYADGTTYELRTPAYTLQNAYEPLPPGVMLSPRVAPPVFGLGLLEAVPE
                     ETILSFADPQDSDGDGISGKPNYVWDVKAHQMALGRFGWKCGAPDLLQQSAGAYNQDM
                     GVTNYLFPQESCSGQPQYDGLNDETEVPDSVLYAAAFYVRTLAVPARRNLDDPRVQRG
                     KILFSSAKCSSCHIPAMQTGTDVSFPEISNQYIFPYTDMLLHDMGPGLADNRPDYDAT
                     GQEWRTQPLWGIGLTQIVNGHQYFLHDGRARSLEEAILWHGGEGEFSKNAFVAMSATD
                     RDALIAFLRSL"
     gene            4701..>5026
                     /locus_tag="VFU15_17245"
     CDS             4701..>5026
                     /locus_tag="VFU15_17245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HEU4719595.1"
                     /translation="MNFLIADSGSTKTDWRYVSGTENISVHTQGYNPYHVTKDKMEES
                     LRADILPRLEGKQADQLFFYGSGCNEKMSPQVKESLRKFFPDADVEVHSDMLGAARGL
                     CGNEKG"
BASE COUNT         1217 a         1377 c         1323 g         1109 t
ORIGIN      
        1 gacaaaccga gccggtggat gctttccgat gcgatgaacg caggactcac cagctacctg
       61 ctgcgcgatt gcggaaaaga agagatcatc gaagcgctga tcactacgtc gaaaggcgaa
      121 caattctttt gcggaaagat cgtgaacgat gtgctgaaaa ccggcgacga tatttccatg
      181 gaaacggaga tcgcgtcatg cgacggcctg aaaatttccg ggcgtgaagc ggagatcatc
      241 cgcctcgttg cggaaggact taccaataag gagatcgcgg acaaactttt tctcagcacg
      301 cacacggtca ccacgcaccg caaaaacatc atggccaaaa ccggcgtgaa caacacggca
      361 ggtctcgtgc tcttcgcgat ccgcaacaac atcatcactc cgaatcattt cctcttcaat
      421 acgaactgac cggaacattc ctgtttacgg ttgagcagcg gcaaaaaaca gccgctgttt
      481 tttttacctg aaacatggta ttgccgtccg cttaccgcac atatattttt gtcgcgttca
      541 attcaacaaa ataaaatgcg acacattatt tctgcagccg ttttttcact tgccatttca
      601 ccggtgtacg cgcaggtgct cacgcacgaa gattcgctca gcgcgggact cgtccgcagc
      661 aattcctcca ctgtcatttc cggttacggt gaagccaggg tcacgtacga tcttatcgat
      721 cgcacgggca acgccgacct cacacgcaac gttctttttg tgggccaccg tttcaaccag
      781 aagatcttca tgttctccga atgggaaatt gaagatgcga aagtggaagg gggcgagccg
      841 ggcggagaga tcgcgatgga acaattgttc ctcaagttca atgtgaaccg tgacctgtat
      901 gtcaatgccg ggcttttcat cccgcgtctc ggcatcatca atgaaaacca tcttcccacg
      961 acattcaacg gcatcgatcg cccttacgtg gaaacgttcg tcattccttc cacgtggcgt
     1021 gaaatgggaa tcgcacttta cgggcagacg ccgcgcttgc ccgggttcaa ctggacggtt
     1081 ggtttgttca acgggctgaa cagtgaagaa ttcggtaacg gaagcggcat ccgcgaagga
     1141 aggttcgaag gaaaaaatgc gagcgcatcg aacctcgcgg tttccgctgc gctgctttat
     1201 taccgggatg cattccgttt ccaggtttcc ggctactacg ggggaactgc aggactttca
     1261 caccgtgacg ccgacagcct gcgtcttgat tcgggtccgt tcggaacgcc cgttgcgctg
     1321 gccgaagcgg atgcgcagta catcagcgaa ggatttacgt tcaaagcgct gggaacgatc
     1381 gttgcgatac cggatgcgca gaatatcaac cgcgcttacg cgaacaacac gccgcagatg
     1441 atgtggggcg cttatgcaga agccgggtac aatgtgcttc atcctttcgc aacgccggaa
     1501 aaaaatcttg tcgtatttgc gcgttacgaa atgctcgatc tcaatgcgaa aatgtccgag
     1561 aacggaatac gggatgaatc gctccggcag caataccttg tgacgggcgt aacatggatg
     1621 ccggtccgcg ggattgccgt gaaagccgat tacacgatgc gtacaaccgg caagcccaac
     1681 ccggcgctgc agataaatcc gtacgtgaac gggcaggtgt tttatgcgac gcgcggattc
     1741 gtcaacctcg gcatcgctta cagcttctga tgatgcagac gcggagacag tttctgaaag
     1801 caggatgcgg gttatgcctc gctcttccgg ggatcggtat cgccgcgtcg ctgctgcagg
     1861 gatgcgcttc cgttccggtt gtgaaagcgt caccgtctga aaataaaatt tccgttgccg
     1921 tttcttcttt cggcaaatcg aactacgtgc tcgtccgttc cgcgaagctg gataacgaca
     1981 tcctgctcgt caaaaaaagt gacggcacat tcaccgcctt gcttatgcaa tgcacgcacc
     2041 aggaacagcc gcttaccgtc agcgggaata cgatcaactg ctccgcgcac gggagcatgt
     2101 tcgatctcga cgggaacgtc acacatgcac ctgccacacg ggcgctgacg aaatacattg
     2161 ctgcacagga gggggaacaa gtcataattc atctcagcta aaaaaacggc aatgaaaaaa
     2221 aacattcttt tcctgttttg cacgactgtg atttttcttt cctgccggaa gcatcacgat
     2281 gatgatcccc agccggcaac atccacgctc actgccgatg tgctcagcga cttgtcggac
     2341 cacgtcatcg gcgccaccta caatgacctt gaagcgcagg cctcggtgct ttaccaggat
     2401 gtgctcaccc tgcaggcggc taccaatgaa accaatcttt cggcctgcag gcaatcatgg
     2461 cgtgaatgtc gttccgcgtg ggagcaatcg gaaggatttt tattcgggcc ggtagaatcg
     2521 cagaacatag acccgcgcat cgatacctgg cccgtaaact tcaacgatct cgattccgtt
     2581 ctcagcagct ccgcagtttt tacttccggc tacatcgaca gcctcgatga tgcgctgaaa
     2641 ggttttcacc ccgttgaata tctccttttc ggcgtgaacg ggaacaaggc agcaaccgat
     2701 ttcacaccgc ggcagctcga tcttcttaca gcgctgacgg aaaacctccg gtcgctcacg
     2761 gcgtcgatcg cggcgcagtg gaacgcggca aaccctaatg gctacaggag ccagtttgtt
     2821 tccgccggtt cagggagcac cgtttacacc acgcaacgtt cagctttcga agaaatggtc
     2881 aatgccatgt caggaatatg cgatgaagtc gccaatgaaa aaatagacgg accgtatgtc
     2941 gcgcaggatc cttcgctgga agaatctccc ttcgccttca actcgacgac ggatttcaca
     3001 aacaacatca aaagcgtccg gaatatttac ctgggttctt ataccagcga tggaaaaggc
     3061 atcgaagacc tcgtcaggca gtacgacctt tcgctcgatg gatcgatcaa gcagaagata
     3121 aacgccgcgc ttgccgcgct cggtacggta accgttccgt tcggccaggc gattgcacag
     3181 caacccgtgc agctgcagaa ttccatgagt gcgatcagtg accttcagca aacgatcgat
     3241 aaccagctta tgccttttat ccaggcccac accaattgaa aatgaagcgg aaattcattc
     3301 tcgcgtcatt gactgtttcc gtagttgcat tcaccatcgc ggacggatgc cgcaagccgg
     3361 atattttcga cgacgcagac ggcaacgaag cgtattcggg cggctcacaa accgttttcg
     3421 acaacggttc cggtgcattc ggacattcct tccctgacat gagcagcgac aagacggaac
     3481 ttcacgctac cggtgatatc gcattcagcg ctcctttcgt cactgcgccg gctccgttgc
     3541 attccgggct tggacccgtg tacaacaatg tttcctgcag caactgccat gtcgccgacg
     3601 gaagaggaaa accgccggaa gccaatgaac aattgctctc catgctcttc cgtatcagca
     3661 tcccgggaac cgacgagcat ggcggaccga atcctgttcc cggtttcgga ggacagcttc
     3721 agcaacgtgc gatcgccggg aaacaaccgg aagctgatgt tgccatttcc tacacgtata
     3781 cgaccgggca atatgcagac ggaacgacgt atgagctgcg cacgccggca tacacattgc
     3841 agaacgcgta tgagccgctt ccgccgggcg taatgctttc gccgcgcgtc gcgccgcctg
     3901 ttttcggact tggcctgctt gaagccgttc ccgaagaaac cattctttcc tttgccgatc
     3961 cgcaggattc tgacggagat ggaatttcag ggaaaccgaa ttacgtgtgg gacgttaagg
     4021 cgcatcagat ggcactgggc cgtttcggat ggaaatgcgg agctccggat ctcctccagc
     4081 agagcgcagg cgcatacaac caggatatgg gcgtgaccaa ttatcttttt ccgcaggaaa
     4141 gctgttcagg tcaaccgcaa tatgacggac tcaacgacga gacggaagtg ccggacagcg
     4201 ttctttacgc agccgcgttt tatgtccgga ccctggcagt tccggcgcgg cgaaacctcg
     4261 acgatccgcg tgtgcagcgg ggaaaaattc tgttcagctc ggcgaaatgc tcttcgtgcc
     4321 atattccggc gatgcaaacc ggaacggacg tttcttttcc ggaaatctcc aaccagtata
     4381 tttttcctta caccgacatg ctgctgcacg atatgggccc gggtcttgcg gataaccgtc
     4441 ctgattacga cgcaacagga caggagtgga ggacgcagcc gctgtgggga atcgggttga
     4501 cgcagatcgt gaacgggcac cagtatttcc tgcacgacgg gcgcgcacgg tcgctggaag
     4561 aagcaatact ctggcatgga ggcgaaggtg aattttcgaa aaacgctttc gttgccatgt
     4621 ccgccacaga ccgtgatgcc ctcatcgctt tcctccggtc gttgtaagaa ccgcttcagg
     4681 cgaaatcgta atttcaccgc atgaattttc tcatcgcgga cagcggctcc acgaaaacag
     4741 actggcgtta tgtttccggg acggaaaata tttcggtcca cacccagggc tataatcctt
     4801 atcacgtgac gaaggataaa atggaagaaa gccttcgtgc agatattctt ccccggctgg
     4861 aaggaaaaca ggcggatcag cttttctttt atggttccgg ctgtaatgaa aaaatgagtc
     4921 cgcaggtgaa agaatcgctg cggaaatttt ttccggatgc agatgtggaa gtgcattccg
     4981 atatgctcgg tgctgcgcgc ggactctgcg gaaatgaaaa aggaat
//
LOCUS       DASTSF010000613         2291 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1246954,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000613 DASTSF010000000
VERSION     DASTSF010000613.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2291
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1246954"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2291)
                     /locus_tag="VFU15_17250"
     CDS             complement(<1..>2291)
                     /locus_tag="VFU15_17250"
                     /inference="COORDINATES: protein motif:HMM:NF024962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprB repeat-containing protein"
                     /protein_id="HEU4719596.1"
                     /translation="NGNAQRTSGPNLDPQSHTVTTVVVPNLCTGQCNATATAVVTGGS
                     GSFSYQWSTVPTQTTQTATGLCAGTYLVVVTDLVNGDQVPAFAVVTDPNPLVIFFFNV
                     SPLCNGQCNGSSTATVAGGTTPYTYQWGPPGNQTTPSISNQCAGTYTLTVTDNNGCVK
                     VDSTTITQPAILAPNGTSANISCFAACDGTAAVAPTGGTPAYSYSWSSGPTTPAIAPL
                     CPGTYTCTVTDSHNCVATYSTTITQPALLQVSLTGTNASCFGVCDGTITSTVSGGTAP
                     YIYSWLPGLQTTPNLNNICAGTYTLNVTDAHGCTAQATITITEPPQLLVNPTGVDITC
                     FGSCNGSAAANVSGGTPGYTYSWLNSGGNGPTASNLCPGTYTVNVNDTNNCSASGTIT
                     ITEPPQLQANASGTNITCFGICNGSATCNPSGGTGAYTYNWQPGNLNTQTISNLCPGS
                     YTLTVHDQNNCTATATITITQPPQLFVNLTTTNVLCNGQCNGTATAAPSGGISPYQYS
                     WQPGGQTTPGISGLCPSSYTINVTDANGCVVSQTFNITQPNPLTVSINSTNINCNSIC
                     NGTAAAAVSGGTPTYSYSWSPGSQTTPGINNLCVGGYTVHVTDANGCQDSANVNIIQP
                     PALTLITGVTNASCSGSCNGSANVNASGGNPPYSYQWAPGGQITPSINNLCAGSYTVT
                     GTDATNCTSTATVTVTEPPVLMANVTSSNVSCNGGANGTATSNPSGGTGPYSFQWSPG
                     GQTTPSITGLSAGTYTCTVTDANG"
BASE COUNT          424 a          574 c          739 g          554 t
ORIGIN      
        1 ccgttcgcat ccgttaccgt gcaggtgtat gttcctgcgc ttaatcctgt gatggaaggc
       61 gtggtttgtc cgccgggtga ccattggaac gaatagggcc cggttccgcc ggaaggattc
      121 gatgttgcag ttccattagc gccgccgttg cagcttacgt tcgacgaggt gacgttcgcc
      181 atcaataccg gaggttccgt cactgttacg gttgcagtgg atgtgcaatt ggtagcatcg
      241 gtccccgtta ctgtatatga gccggcgcaa agattgttga tcgaaggtgt gatttgtccg
      301 cccggcgccc attggtagct ataaggggga ttgccgcccg aagcatttac gttggcgctt
      361 ccgttgcagc ttccgctgca tgatgcattc gtaaccccgg ttattaatgt gagcgcaggc
      421 ggctgaatga tgtttacgtt agccgaatcc tggcacccgt tggcatccgt cacgtgaacg
      481 gtataaccgc ccacgcagag attattgatg cccggcgtag tctgtgatcc cggcgaccag
      541 gaatagctgt atgtcggtgt tccgccggac acagcagcgg ccgcggttcc gttgcagatg
      601 ctgttgcagt taatgttggt ggaattgatc gaaaccgtga gcgggttggg ttgtgtaatg
      661 ttgaatgttt gtgaaaccac gcagccattg gcgtccgtca cgttgatcgt atacgagctg
      721 ggacacaggc cggaaattcc cggtgtagtc tgtccgcccg gttgccagct gtactggtag
      781 ggggaaattc ccccggaagg cgcagccgtc gcggttccgt tgcattgccc gttgcacaat
      841 acgtttgtcg tggtgagatt gacaaacagc tgcggaggtt gcgtaatggt gatcgttgcg
      901 gtggcggtac aattgttctg gtcgtggacg gtcaatgtat atgatcccgg gcaaaggttc
      961 gagatggttt gtgtattcag gttgcccggc tgccagttgt aggtgtatgc acctgttcca
     1021 cccgaaggat tgcacgtggc agatccgttg cagataccga agcaggtgat gttggtgccg
     1081 cttgcgttcg cctgcagctg gggcggctcc gtgatcgtga tcgttccgct ggcggagcag
     1141 ttgttggtgt cgttcacgtt cacggtatat gtgcccgggc acaggttcga tgccgtaggt
     1201 ccgttgccgc cgctgttcaa ccagctgtat gtataaccgg gtgttccgcc cgaaacgttt
     1261 gccgcggcgg acccgttgca cgatccgaaa caggtaatgt caacgcctgt cgggttcacc
     1321 agcagctgcg gcggttcggt gatcgtgatc gtcgcctgtg cagtgcatcc gtgcgcgtcg
     1381 gtaacattca gcgtataggt tccggcacaa atgttattca ggttcggtgt cgtctgcaga
     1441 cccggcagcc aggaatagat gtacggcgca gtaccaccgc tgactgtgct cgttatcgtt
     1501 ccgtcgcaga caccgaagca gcttgcattg gttccggtca gcgacacctg cagcaatgcg
     1561 ggctgggtga tggtggtcga ataggtcgcc acgcaattat gcgaatctgt taccgtgcat
     1621 gtgtacgttc cgggacacag cggagcaata gccggtgtcg ttggtccgga tgaccacgaa
     1681 tacgaataag cgggagttcc gccggtgggt gcgacagcag ccgtaccatc gcatgcggca
     1741 aagcaggaaa tgtttgcgct cgtgccgttg ggagcaagaa tggccggttg cgtgatcgtt
     1801 gtcgaatcca cttttacgca gccattgttg tctgttaccg taagcgtgta ggttcctgcg
     1861 cactgattcg agatggacgg tgtcgtctgg tttcccggcg ggccccattg gtacgtatac
     1921 ggcgttgttc cgccggcgac ggtagccgtg ctcgatccgt tgcactggcc gttgcaaagc
     1981 ggggaaacat tgaagaagaa aatgacaagc gggttcgggt cggtgacgac agcgaaggcg
     2041 ggaacctggt cgccgttcac aagatcggtt accacgacga gatacgttcc tgcgcagagg
     2101 ccggtggctg tctgcgtggt ttgcgtcgga acagtgctcc actggtagga aaagcttccg
     2161 gagccgccgg taacaacagc cgtggcggtt gcgttgcatt gtcccgtgca gaggttggga
     2221 acaacaacgg tggtgacagt atgcgattgc ggatccaggt tcggaccgga agtgcgctgc
     2281 gcattcccgt t
//
LOCUS       DASTSF010000614        10907 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_115545,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000614 DASTSF010000000
VERSION     DASTSF010000614.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 10907)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10907)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10907
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115545"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..774
                     /locus_tag="VFU15_17255"
     CDS             <1..774
                     /locus_tag="VFU15_17255"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEU4719597.1"
                     /translation="FAVHFHEFRSQFNYISAEELMLQTQEAKFLVYCSNAVGEALNTI
                     GVKNKYLLYEAVDLAAIQPDHERTQQLRKELGISPSAKVIVMSGSQAEIKGIDIFVRV
                     AEQLKSEPLHFLWLGSPVKSAFSWFWTEYAKKSAANITFCHPAPADYYPGLALADLFF
                     LSSRTDPFPLVMIEAAYLGKFIAGLDSGGVSEFVQDGCGVILPTWNADEIAGRVRDLC
                     AKLPAGWSPEKSTARAKQFDSKDLRGKFEQVMDAVFGNP"
     gene            775..1731
                     /locus_tag="VFU15_17260"
     CDS             775..1731
                     /locus_tag="VFU15_17260"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU4719598.1"
                     /translation="MQKRNMPGTTVDILLSTFNGEQHLAEQLESLLKQDFTGWKLIVR
                     DDASGDGTFGILRQYADRFPGKIDLSDDRAKLGFSSSFAELLKRSSAEYVMFCDQDDS
                     WNSDKISTLLALIRREEKKLPGIPHLVFSDLSLMDENGESIRGSFLALTGYEEKYGQE
                     QFFLRNYVPGCSLLFNRALAEKALAVPDHLGYHDHRVIFTCAATGKITYCDRALMHYR
                     LHGKNAIGLSERKSESFSVGFKTLVKFIFANRAYRNVRYGANLRQMKETGDRFANELT
                     EPALRLYKWEDAGYLRRKRWNLSAPFVHEKTLTERLVYFFCF"
     gene            1742..2626
                     /locus_tag="VFU15_17265"
     CDS             1742..2626
                     /locus_tag="VFU15_17265"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU4719599.1"
                     /translation="MDVSVIIINYNTYALTSRCIASVFEFTKDLSFEIILVDNASTEC
                     DPGKFTEEFPGITLVRNAENTGFAKGNNTGIEKASGKYILLLNSDTELKENSIRKCIG
                     QFAQEPAPGIVTCRLVYPDGRAQFQCQRFPSVALCLLELFRLHKLLSAEKKAKILSGP
                     FFSHTENAWPESVWGTFFLFPAKILRELPGEKLPDDFFMYGEDLEWCYVFRKKGYRAF
                     YYAGTSVIHHHGASASEAVLEHKHRNEFFFLRKYRGDFYARTLMFFRSLLWLTSRDPL
                     AGKIRRISFRLFLKGAAA"
     gene            2623..3522
                     /locus_tag="VFU15_17270"
     CDS             2623..3522
                     /locus_tag="VFU15_17270"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU4719600.1"
                     /translation="MKTSVIIPTYSGGEKLATTVKSLCAGTARFEELIIVVDNDDAHV
                     VVPDHTVTKTGTTLIVIRQKNSGRAAARNAGASAATSGLLLFIDDDMICSADLVSKHV
                     QFHETNPACILSGSGFRNPAEAKSGFDRFIIRQEESWRTSMIAKGEVTFEKFSFTACN
                     LSLPREVFSLLRGFDEELSDAEDLDFGVRALQKDIRVFYDGGIIAFHNDFPSPGDFIR
                     RSNEYAKARTALLRLRPDYQQLLPSLVIPKNGPLKRFFLRIAKKTVCRGVLENGPLFR
                     LLPLRMKFFFYRLTLSAFSVVNE"
     gene            3515..4657
                     /locus_tag="VFU15_17275"
     CDS             3515..4657
                     /locus_tag="VFU15_17275"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEU4719601.1"
                     /translation="MNERPLHIVHVMANNSSVPYFNWFAEKAQEEPNVQVSFVALCAE
                     RPQMIEDMDRRGSKAYWIPFRNTSRGRSMLAAVPKLVRLFRELKPDVVHAHLFDDAVP
                     ALAAARIAGVKVRVITKGDTGFHWYFAPKGIKFDRLNNRNANLIFALSQESRDFILEK
                     EKADPRKVKIVHHGIPVAQVTHQAAEARDRLRARFGIREGDIVIGNVSRFIEWKGHRH
                     IIAAAEMLVKENPRLRFLFAGTGDLQQEMMALVRKKNLAGNIQLTGWIERSDMPSFYG
                     IMDIYLHAAAMEPFGFVIAEAMMNAVPVVSTSTGAARDAIESGKNGYLVSAQNAEALA
                     EGIRYLLSVDRKKTGEAGRQTALQLFSLDRMWQDHLRLYREALNQN"
     gene            4657..5523
                     /locus_tag="VFU15_17280"
     CDS             4657..5523
                     /locus_tag="VFU15_17280"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU4719602.1"
                     /translation="MPLVSIVTPAYNAAKFIGETIRSVQAQTISDWEMIIINDGSRDN
                     TAEIVEAFLADTRITIHHQANAGVSAARNNGLSKAKGAFIALLDADDTFLPDNLEKKT
                     GALQKDPEAVWTYSNLRLIGEDSNACGPDTNGKGENVLENLLLWNGEVIPGPCSNLVF
                     RRRVIDEGVKFDTAFSTAADQDFCLQLAQKYKSVFLREVLACYRILPGSMSRNIAVME
                     KDHTGVYEKAKRSGAFSSGAFRRKCFSNLYLILAGSWWVNGNNKKRGLLFMWKAIIAW
                     PPAFFSLLKKLT"
     gene            5523..6719
                     /locus_tag="VFU15_17285"
     CDS             5523..6719
                     /locus_tag="VFU15_17285"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU4719603.1"
                     /translation="MARNILVLTYWSFDDPLIGIYTLPYLRIIRDILPEDSRIHLLTL
                     ERDGESEQENGIEKGIFHFSFALHPFGANALFSWMKNIRKLRRFIRTNKIDTIHTWCT
                     PAGGIGWRLSKKTGIPLVLDSFEPHADPMVETGTWKKNGIAFRVLSFLEKKQVNHAKW
                     VIGVVPGVKKYAQEKYGYSGNNFFAKPACIDTQRFNVTERKNPELLRSLGLENKIVCV
                     CAGKFGGLYLEEEASGFFRQAFEKWRDDFRLLLLTSAPRETVEKITGAAGIPAGNVVS
                     LFVPNEKMPAYLGLADFAFSHYKPVPSRKYCTPIKDGEFWGVGLPVVIPAGISEDSDI
                     IAQNNAGYVLRSFSGEEFRNAVERIDELIRKDSDGAMAKRIHELTVKHRNFTAATDIY
                     RKIYSA"
     gene            6716..7753
                     /gene="galE"
                     /locus_tag="VFU15_17290"
     CDS             6716..7753
                     /gene="galE"
                     /locus_tag="VFU15_17290"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020526799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEU4719604.1"
                     /translation="MSRKTVLVTGGAGYIGSHTIVALAEQPDCAIVSADNYSNSSAKT
                     YDRIERITGKKIQHMECDLSDAEQVKALFGKFPGITGVIHFAAFKSVPESVAFPLMYY
                     RNNVNSLINLLETAEKAGATDFIFSSSCSVYGNISSLPVNEMTVPGKTESPYAFTKVI
                     GEKILQDFTAVSKKMKGISLRYFNPVGAHETGLLGELPNQRPNNLVPVITQTAIGKIP
                     EMQVFGSDYGTRDGTCIRDYVHVCDIAGAHVKALDFLEKNASAKKYDVFNLGSGSGVT
                     VLEAIQAFENVSRRALNYKVAPRRAGDVEAIFSDSSKAEKELGWQAKRSLREMMESAW
                     KWEQHLAAEGA"
     gene            complement(7758..9209)
                     /locus_tag="VFU15_17295"
     CDS             complement(7758..9209)
                     /locus_tag="VFU15_17295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719605.1"
                     /translation="MFEVETFSYLKQIYPFEWVMLTIYMLVLLGISFFIQVSNIKKQP
                     VYRYYLGGVAMKAFSAVVFCLIYIYYYNGGDTVCYYETSRALVNLGMKDPASFWKVMT
                     TAPSVENYFLFDDKTGFPWAFMYYDTQTFFVAKLIAPILFVAFRSYLVSGVVLSWLSF
                     FGIWRFFLMLCRHYPAIEKRLALGVIFVPSVLFWGSGILKDTITFSAVCWFIVGIEGF
                     FILKKSRLKNAAIFIVASYVLISIKAYIMISLLPGVFLWLLYSRFSRFKSYFLRYMSV
                     PFIVIFSLAAGYGLLSVLGDSLGKFSVAHMLETASITQRDLKQDYYHGSSFDIGEFDP
                     TISGVISKAPAAMAVGLFRPFIWEAHNVVMLVSGLENLVYLSLTLMLFARFIMQPKKF
                     FRTFFNDPLLLFMFTYTLLFSVLVGLSTSNFGALVRFKIPFLPAFICFLLILNYKLAR
                     KRSGAPAGDYSPLVNPGTIRYDEKTGIITKAGR"
     gene            complement(9213..9686)
                     /locus_tag="VFU15_17300"
     CDS             complement(9213..9686)
                     /locus_tag="VFU15_17300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016777664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEU4719606.1"
                     /translation="MKIAFLFTGKTDESWVQEGLDTYARRLVHYCTPEVITLLPPRNT
                     ARGTPAEVKSAEGRLILSKLLPGDRLVLLDEAGKSYSSQEFSAWIGKQQLAGVKRVVF
                     ATGGAFGFSEEVYARADEKLSLSKMTFTHQMVRVIFAEQVYRAFTILRGEKYHHA"
     gene            9785..10168
                     /locus_tag="VFU15_17305"
     CDS             9785..10168
                     /locus_tag="VFU15_17305"
                     /inference="COORDINATES: protein motif:HMM:NF027349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4783 domain-containing protein"
                     /protein_id="HEU4719607.1"
                     /translation="MKTVLALLLFLAPFAASADVADDVAQAIRTGNAAEVSKYFAESV
                     DLKVLDQENIYSKAQAELILKDFFDKHPVTEFTVVHKSLPKNDSQFATGTLKTSNGTY
                     RVHYLMKTAGASTTVTQFRIENSDE"
     gene            10161..>10907
                     /gene="nadC"
                     /locus_tag="VFU15_17310"
     CDS             10161..>10907
                     /gene="nadC"
                     /locus_tag="VFU15_17310"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010600200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEU4719608.1"
                     /translation="MNKRTGYTPSSPDLSLIRDFIASALHEDVGEGDFTSLSTIPADA
                     ADKAKLLVKDDGVLAGVELAGMIFRQVDPALSIDVLISDGEFVRKGDVAFTVRGPARS
                     ILIAERLVLNCMQRMSGIATVTWELVKLLDGLHTKVIDTRKTTPGFRYFEKWAVRIGG
                     GTNHRFGLYDMILIKDNHVDYAGGIRNAITSAQRFLHEKELRIPVEIEVRNFRELGEV
                     LSTGGVDRILLDNFSVPDLAKAVKIIGGKFR"
BASE COUNT         2676 a         2964 c         2885 g         2382 t
ORIGIN      
        1 ttcgccgttc atttccatga attccgcagc cagttcaatt acatttccgc tgaggaactt
       61 atgctgcaaa cgcaggaggc gaaattcctg gtgtattgtt ccaacgcagt aggcgaagcg
      121 ctgaacacga tcggcgtgaa aaacaaatac cttctttacg aagcggtgga ccttgcggct
      181 atccagccgg accacgagcg cacacagcag ttacggaaag agctcgggat ttctccgtcg
      241 gcgaaggtta tcgtgatgtc cggctcgcag gcggagatca aaggcatcga tattttcgtg
      301 cgcgtggcgg aacagctgaa gagcgaaccg ctgcatttcc tgtggctggg ttctccggtg
      361 aaaagtgcgt tttcctggtt ctggacggaa tacgcaaaaa aatcagcggc gaacatcacg
      421 ttctgccacc cggcgcctgc cgattattac ccgggcctcg ccctcgccga tctttttttc
      481 ctcagctctc gcacggatcc ttttccactg gtcatgatcg aagccgctta ccttggaaaa
      541 ttcattgcag gtcttgattc cggcggcgtt tcggaattcg tgcaggacgg ctgcggcgtt
      601 attcttccca cgtggaatgc ggatgagatc gccggccggg tacgcgacct gtgcgcgaaa
      661 cttccggcgg gctggtcccc ggaaaaatcc acggcgcgtg cgaaacaatt cgacagcaaa
      721 gaccttcgcg gaaaatttga acaggtaatg gatgccgttt tcggaaatcc ataaatgcaa
      781 aaacgcaaca tgcccgggac cacggtggac atcctgcttt caaccttcaa tggtgaacaa
      841 caccttgcgg agcagctgga aagtctgctg aagcaggact tcaccggatg gaaactgatc
      901 gttcgggatg acgcttccgg cgacggaaca ttcggtattc tccggcaata cgctgatcgt
      961 ttcccgggca agatcgatct ttccgatgat cgcgcgaagc tgggattctc atcgtcattc
     1021 gcagaattgc tgaaacggtc atcagcagaa tacgtgatgt tctgcgacca ggacgattca
     1081 tggaacagcg ataaaatttc cacgctgctc gcgctcatcc gcagggaaga aaaaaaactc
     1141 cccgggatcc cgcacctcgt tttttccgat ctctcgctca tggatgagaa cggtgaaagc
     1201 attcggggct cctttcttgc gctgaccggt tacgaggaaa aatacgggca ggagcagttc
     1261 tttctccgca attacgttcc gggctgcagc ctgctgttca accgggcgct tgcggaaaaa
     1321 gcgctggccg ttccggatca tctcggctac cacgaccatc gcgtcatttt tacctgcgct
     1381 gcaaccggga agatcaccta ctgcgatcgt gcattaatgc actaccgcct gcacggcaaa
     1441 aatgcgatcg gcctttcgga aaggaaaagc gaatcttttt ccgtcggctt caaaacattg
     1501 gtaaaattca ttttcgccaa ccgcgcttat cgcaatgtcc gctacggcgc caacctgcgg
     1561 cagatgaaag aaaccggcga tcgttttgca aatgagctga cagaaccggc gctgcggctg
     1621 tacaaatggg aagatgccgg ttacctgcgc cgcaagcgat ggaacttatc agcgccgttt
     1681 gtgcacgaaa agacgctgac ggaacggctc gtttacttct tctgttttta attttacgcc
     1741 ggtggatgta tccgtcatca ttatcaatta caacacgtac gcgctgacca gcaggtgcat
     1801 cgcttccgtg tttgaattca cgaaggatct ttcattcgag atcatccttg tcgataatgc
     1861 atccaccgaa tgcgatcccg gaaaattcac cgaagaattc ccgggtatta cactggtgag
     1921 gaatgcggaa aatacaggct tcgcaaaagg caacaacacc gggatagaaa aagcttccgg
     1981 gaaatacata ttgctgctca acagcgacac ggaactcaaa gaaaattcca tccgcaaatg
     2041 catcgggcag tttgcgcagg agccggcgcc cgggatcgtc acgtgcaggc tggtttatcc
     2101 cgatggcagg gcacagttcc agtgccagcg ttttccttcg gtcgccttgt gcctgctcga
     2161 gctcttccgc ctgcataaac tgctttccgc ggaaaaaaaa gcaaagatcc tttccggtcc
     2221 ttttttcagt cacacggaaa atgcgtggcc cgaatcggtg tgggggacat tcttcctttt
     2281 cccggcgaag atcctccgcg agctgccggg cgaaaagctt cccgatgatt ttttcatgta
     2341 cggcgaagat ctcgaatggt gctatgtttt ccgcaagaaa ggttaccgcg cattttatta
     2401 tgccgggaca tccgttatcc accaccacgg cgcatcggct tcggaagccg ttctcgaaca
     2461 caagcaccgg aatgaattct ttttccttcg gaaataccgc ggcgattttt atgcgcgcac
     2521 actgatgttc ttccgctccc tgctctggct tacttcacgt gatccgcttg ccgggaagat
     2581 ccgcaggatc tcgttccgtc tttttctgaa aggagcggct gcatgaagac gagcgtgatc
     2641 attcccactt attccggcgg agaaaagctg gcaacaactg taaaaagcct ttgcgcggga
     2701 actgcgcgtt tcgaagagct gatcatcgtg gtggacaacg acgatgctca tgtcgtggtc
     2761 ccggaccata cagtgaccaa aacaggcaca acgctcattg tcatccggca gaaaaattcc
     2821 ggtcgcgctg ccgcacgaaa tgcaggcgct tccgcggcca catcaggtct tcttcttttc
     2881 atcgacgacg acatgatctg ctcagcggac cttgtttcaa agcatgttca attccacgaa
     2941 acaaatcccg cgtgcatttt atccggaagc ggtttcagga atccggcgga agcaaaatca
     3001 ggcttcgacc gcttcatcat ccggcaggaa gaaagctggc ggacatcgat gatcgccaaa
     3061 ggagaagtga cgtttgaaaa attttcgttc acagcctgca acctttccct tccgcgggaa
     3121 gttttttccc tgctccgcgg gtttgatgaa gaattatccg atgcggaaga cctcgatttc
     3181 ggtgttcgcg ccttacaaaa agacatccgt gttttttatg acggcggaat aattgctttc
     3241 cacaacgact tcccttcacc cggagatttc atccgccgca gcaatgaata cgcgaaggcg
     3301 cgcacagcgc tgctccgtct ccggccggat taccagcaat tacttccttc gctcgttatt
     3361 ccgaagaacg ggccgctgaa acgttttttc cttcgcatcg ccaaaaaaac agtttgccgg
     3421 ggcgtcctgg agaacggacc tttgttccgc cttcttccgc tgcgcatgaa attctttttt
     3481 taccgtctta cgctttctgc attttccgtc gtcaatgaat gaacgtccgc tccatatcgt
     3541 tcacgtaatg gccaacaaca gctcggtccc gtacttcaac tggttcgcgg aaaaagcgca
     3601 ggaagaaccg aatgtgcagg tttccttcgt cgcgctttgc gcggaacgtc cgcaaatgat
     3661 cgaagacatg gaccggcgcg gaagcaaagc gtactggatc ccgttccgga acacgagccg
     3721 cggacgctcc atgctggctg ccgtgccgaa gctggtaaga cttttccggg aactgaaacc
     3781 ggacgtcgtg cacgcacatc ttttcgatga cgctgttccc gcgctggccg ctgcacgcat
     3841 tgccggtgtg aaagtccgcg tgatcaccaa gggcgacacc ggttttcact ggtatttcgc
     3901 cccgaaagga ataaaattcg accgcctcaa taaccgcaac gccaatctca tcttcgcgct
     3961 ctcgcaggaa agccgcgatt tcatcctcga aaaagaaaaa gccgatccgc gcaaagtgaa
     4021 gatcgttcat cacggtatcc cggtcgcgca ggtgacgcac caggccgcgg aagccaggga
     4081 ccgccttcgt gcacggttcg gtatccgcga gggcgatatc gtcatcggta acgtgtcgcg
     4141 ttttatcgaa tggaaaggcc accggcatat tatcgctgca gcggaaatgc tggtgaaaga
     4201 aaatccgcgg ctgcgctttt tatttgccgg caccggcgac ctgcagcagg agatgatggc
     4261 gctggtgcgc aaaaaaaatc ttgcagggaa catccagctc acgggatgga tcgaacgcag
     4321 cgacatgcct tcgttttacg gcatcatgga tatttatctt cacgccgcgg caatggaacc
     4381 tttcggtttc gttatcgcgg aagcaatgat gaacgctgtt cctgtcgtct ccacttccac
     4441 gggcgccgca cgtgatgcga tcgaaagcgg gaaaaacggc tatcttgttt ccgcacagaa
     4501 tgcagaggcc ctggcggaag gaatccgtta cctgctttca gtcgaccgga aaaaaaccgg
     4561 ggaggcaggc aggcagaccg cgctgcagct gttcagtctc gaccgcatgt ggcaggatca
     4621 cctgcggctt taccgcgaag cgttaaacca gaactgatgc cgctcgtttc gattgttacg
     4681 cctgcttaca acgccgcgaa attcatcggt gagaccatcc gttccgttca ggcgcagacg
     4741 atctccgact gggaaatgat catcattaat gacggttcac gcgacaacac cgcggaaatt
     4801 gttgaagctt ttctcgccga cacacgcatc acgatccatc accaggcgaa cgccggtgtg
     4861 agcgcggcac gcaacaacgg gctttcaaaa gcaaaaggag cattcatcgc gctgctcgac
     4921 gcagacgata cattccttcc ggacaacctc gaaaagaaaa caggagcgct gcaaaaagat
     4981 ccggaagcag tgtggaccta cagcaacctc cgtcttatcg gcgaagattc gaatgcctgc
     5041 ggccccgaca cgaacgggaa aggagaaaac gtgctggaga atctactgct gtggaacggt
     5101 gaagtgatcc cgggcccttg cagcaatctt gttttccgcc gccgcgtcat tgacgaaggc
     5161 gtgaaattcg acacggcatt ttccaccgcc gccgaccagg atttctgcct gcaactggcg
     5221 cagaaataca aaagcgtttt tctccgtgaa gtcctcgcct gctatcggat tcttcccggc
     5281 agcatgagcc gcaacatcgc ggtaatggaa aaagaccaca ccggcgttta tgaaaaagcg
     5341 aaacgcagcg gtgcattttc ctccggcgca ttccggcgga aatgcttttc gaacctttat
     5401 ctcattctcg ccggcagctg gtgggtgaac ggcaacaata aaaaacgggg attgcttttc
     5461 atgtggaaag caattattgc ctggccgccc gcatttttca gtctcctaaa gaaattaacc
     5521 tgatggcgcg gaatatcctg gtgctcactt actggtcgtt tgacgatccg ctcatcggga
     5581 tctatacgct gccgtacctg cgcatcattc gtgatattct ccccgaagac agccgcattc
     5641 acctgctcac gctcgagcgt gacggcgaaa gcgaacagga gaacgggatc gaaaaaggca
     5701 tttttcattt ttcatttgcg ctgcaccctt tcggggcgaa tgcgcttttt tcctggatga
     5761 aaaacatcag gaagctgcgc cggttcatcc gcacgaataa gattgatacg atacacacct
     5821 ggtgcacacc agcgggcgga atcgggtggc ggctttcaaa aaaaacagga attcccctcg
     5881 tgctcgacag cttcgagccg cacgccgatc ccatggtgga aacgggcaca tggaaaaaga
     5941 acggtatcgc tttccgtgtt ctttccttcc tcgagaagaa acaggtgaac cacgcgaaat
     6001 gggtgatcgg cgtcgtgccg ggcgtgaaaa aatacgcgca ggaaaaatat ggctacagcg
     6061 gaaacaattt tttcgcgaag ccggcctgca tcgatacaca gcgtttcaat gtgacggaac
     6121 ggaagaaccc ggagctgctg cgttcgctcg gactggaaaa caagatcgtc tgcgtgtgcg
     6181 cggggaaatt cggcggactt tacctcgaag aagaagcgtc cggatttttc cggcaggctt
     6241 tcgaaaagtg gcgggacgat ttccgtttgc tgctcctcac gtctgcgccg cgcgaaacgg
     6301 ttgaaaaaat tacgggtgct gcggggatcc cggcaggaaa cgtggtatcc cttttcgttc
     6361 ccaatgaaaa aatgcccgct tacctcggtc tcgccgattt cgccttctcg cactacaagc
     6421 ccgttccaag ccggaaatac tgcacgccta tcaaggacgg ggaattctgg ggcgtgggac
     6481 ttccggtcgt tattcctgca ggcatttcag aagacagcga tattattgca cagaataacg
     6541 cggggtacgt cctgcgctcc ttttccggcg aagaattccg gaatgctgtg gaaaggatcg
     6601 atgagctgat ccgaaaagac agcgatggtg cgatggcaaa acgtatccat gaattaacag
     6661 taaagcacag gaattttacg gcggcaaccg atatttacag aaaaatatat tccgcatgag
     6721 caggaaaaca gtacttgtca ccgggggcgc cggctacatc gggtcgcata ccatcgtcgc
     6781 actcgccgaa caaccggact gcgctatcgt ttctgccgac aactattcga attcttcagc
     6841 gaaaacgtac gatcggatag aacgcattac cgggaaaaaa atccagcaca tggaatgcga
     6901 tctttccgat gcggaacagg tgaaggccct gttcggaaag ttcccgggca tcaccggcgt
     6961 gatccatttc gccgcgttca agtcggtccc ggaatcggtc gcgttcccgc tgatgtatta
     7021 ccggaacaat gtcaattcac tgatcaacct tctcgaaaca gcggaaaaag caggtgcgac
     7081 ggatttcatt ttttcctctt cctgctccgt ctacggaaat atttcctcgc ttcctgtcaa
     7141 tgaaatgacc gttccgggaa aaacggaatc accttatgcg ttcaccaaag tgatcggcga
     7201 aaagatcctc caggatttca cggccgtttc gaaaaagatg aaaggcattt cgctgcgcta
     7261 tttcaacccg gtcggcgcgc acgaaaccgg cctgctcgga gagcttccga accagcgccc
     7321 gaacaatctc gttcctgtca tcacgcagac cgccatcggg aagatcccgg aaatgcaggt
     7381 gttcggcagc gactacggca cgcgcgacgg cacgtgcata cgcgattatg tgcacgtgtg
     7441 cgacatcgcg ggggcgcatg tcaaagcgct tgactttctt gaaaaaaacg cttcagcgaa
     7501 aaagtacgac gtgttcaacc tcggcagcgg aagcggcgtt acggtgctgg aagcgataca
     7561 ggcattcgag aacgtttccc gccgcgcgct taactacaag gttgcgccgc gccgcgccgg
     7621 cgatgtggaa gccatttttt ccgattcatc gaaagcagaa aaagagctcg gctggcaggc
     7681 gaaacgttcg ctgcgggaga tgatggaaag cgcgtggaaa tgggagcagc acctagctgc
     7741 ggaaggagcc tgaattttca gcggcccgct ttggttatta ttcctgtttt ttcatcgtaa
     7801 cggatcgtcc cggggttcac cagcggggaa taatcacctg caggagctcc ggacctcttc
     7861 cgggcgagct tgtaattcag gatcagcaag aagcagataa aggccggcag gaacgggatc
     7921 ttgaatcgca ccagcgcgcc gaaattggac gtggaaagcc cgacaagcac ggaaaaaagc
     7981 aatgtgtagg tgaacatgaa aagcaggagc ggatcattga agaacgtgcg gaagaatttt
     8041 ttcggctgca tgatgaatct cgcgaaaagc atcagcgtca gcgagaggta gaccaggttc
     8101 tcgagtcctg aaacgagcat caccacgtta tgcgcttccc agatgaacgg ccggaaaagt
     8161 cccacagcca tggccgcggg cgccttgctg atcacgccgg aaatggtagg atcgaattcc
     8221 ccgatgtcga aagagcttcc gtggtaataa tcctgcttca ggtcgcgttg cgtaatcgaa
     8281 gcggtttcca gcatgtgcgc cactgaaaat ttcccgaggc tgtcgccgag caccgaaaga
     8341 agtccgtacc cggccgccag cgaaaagatc acgatgaacg gcacggacat gtagcgcagg
     8401 aaatacgatt tgaaccggga aaaacgggaa tacagcagcc agaggaaaac cccgggaagc
     8461 agcgagatca tgatgtacgc tttgatcgag atcagcacgt acgatgcgac aataaaaatg
     8521 gccgcgttct tcagccgtga ttttttcagg ataaagaatc cttctatccc aacgataaac
     8581 cagcatacgg cggaaaatgt gatcgtgtct ttcaggatgc cggaacccca gaacagcacc
     8641 gacggaacaa aaatgacgcc cagcgcaagt cttttctcaa tagcgggata atggcggcaa
     8701 agcatcagga agaagcgcca tatcccgaaa aaagaaagcc aggaaagcac cacgccggaa
     8761 acgaggtagc tgcggaacgc aacgaaaagg atgggtgcga tcagctttgc gacgaagaac
     8821 gtctgtgtgt cgtagtacat gaacgcccag ggaaatcctg ttttgtcgtc aaacaggaaa
     8881 taattctcca ccgaaggcgc cgtcgtcatc accttccaga aactcgccgg gtctttcatg
     8941 ccgagattaa cgagcgcccg cgacgtttcg taatagcaga ccgtgtcgcc gccattgtag
     9001 taataaatgt agatgaggca gaagacaacc gcgctgaacg ctttcatggc tactccgccg
     9061 agataataac ggtagaccgg ctgttttttg atgttcgaga cctggatgaa aaaagagatg
     9121 ccgagcagca cgagcatata gatcgtcagc atgacccatt cgaaaggata gatctgcttg
     9181 agataggaaa acgtttctac ttcgaacatc gctcaggcgt gatggtattt ttccccgcga
     9241 aggatggtga atgcacggta cacctgctcg gcgaagatga cgcgcaccat ctggtgggtg
     9301 aacgtcattt tcgaaaggga cagcttttca tcggcgcggg catacacttc ttccgaaaag
     9361 ccgaaggcgc cgcccgtcgc gaaaacaacg cgctttacgc cggccagctg ctgcttccct
     9421 atccatgccg aaaattcctg cgagctgtag ctcttcccgg cttcatccag cagcaccagc
     9481 cggtcgccgg gcagcagctt cgaaaggatc agccggcctt ccgcgctttt tacctctgcc
     9541 ggcgttcccc gggctgtgtt gcggggaggc agaagcgtga tcacctcggg tgtgcagtaa
     9601 tgcaccaggc gccgcgcata ggtgtcgaga ccttcctgca cccaggattc atccgttttt
     9661 cccgtgaaaa ggaaggcgat tttcattcgg ctaaaacctt atttttgtgc ccgacacagc
     9721 accactgcta aattacctca aatttggctc gaaagttgtt actgaaccgg taaatgaaac
     9781 tgagatgaaa accgtgcttg ctcttctgtt gttccttgcc ccctttgccg catccgccga
     9841 tgtggctgac gatgtcgccc aggcgatccg taccggcaat gccgcggaag tttccaaata
     9901 ttttgctgaa agcgtcgacc tgaaagtcct cgaccaggaa aatatttaca gcaaagcgca
     9961 ggcggagctc atcctgaaag atttcttcga caaacacccg gtgacggaat tcacggtcgt
    10021 acacaaaagc cttcccaaga acgattcgca gtttgcgacg ggaacgctga aaacatccaa
    10081 cggcacttac cgtgtacatt acctgatgaa aacagccgga gcctcgacca cggtaacgca
    10141 gttccgaatc gagaactctg atgaataaac gcaccgggta cacaccatct tctcctgacc
    10201 tttccctgat ccgcgatttt atcgcatctg cgttgcacga agatgtcggc gaaggcgatt
    10261 tcacttccct ctccaccatt cccgcagatg cagccgataa ggcgaaactg ctcgtcaagg
    10321 acgacggcgt gctcgccggt gtggagctgg ccggaatgat cttccgccag gtcgatcccg
    10381 cgctttccat tgatgtgctg atcagcgacg gggaatttgt ccgcaaaggt gatgtggcgt
    10441 ttacggtgcg gggaccggcc cgttccatac ttattgcgga acgtctcgtg ctgaactgca
    10501 tgcagcgcat gagcggcata gcaaccgtaa catgggagct ggtgaagctg ctcgacgggc
    10561 tgcacacaaa agtgatcgat acacgcaaaa caacaccggg cttccgctat ttcgaaaaat
    10621 gggccgttcg catcggcggc gggaccaatc accgtttcgg gctgtacgac atgatcctta
    10681 tcaaggacaa ccacgtggat tacgccggcg gaatacgcaa cgccattacc tccgcgcaac
    10741 gttttcttca tgaaaaagag ctgcgcattc ccgtcgagat cgaagtgcgc aatttccggg
    10801 agctcgggga ggtcctttcc accggcggcg tcgaccgcat cttgctcgat aatttttccg
    10861 tgcccgatct cgcgaaagca gtgaagatca tcgggggaaa attccgg
//
LOCUS       DASTSF010000615         2236 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_799575,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000615 DASTSF010000000
VERSION     DASTSF010000615.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2236
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_799575"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1120
                     /locus_tag="VFU15_17315"
     CDS             <1..1120
                     /locus_tag="VFU15_17315"
                     /inference="COORDINATES: protein motif:HMM:NF024973.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HEU4719609.1"
                     /translation="VGETMYKEFVFPNTAGKLMLPSYDYSCSVKQNPMPTGNDFIDDI
                     MSIDMPVTLHSEPVEITALPVPDANKPADFLGDVGKYALSASIDRQKVKTGEPVKLTV
                     MITGRGNVSFLRLGDQHFPPGIEPYPVVNNDSLVITGSGVTGSKNFTFTLIPKKEGDY
                     TISGITFSYYDVSKKEYVTLKTPDFSLHADKNENEKTEEENNLPKGFPVADNSDFLFG
                     LKVVALLFLISGGIWYYYARNKREVEKQRMESEKKQVPVEEPVFTVTKPVVKNNLETA
                     SFMISSGNYSGAVKCMYDELVSSLCRCCEFSPEEFSVSQVRYRMQMKKFSSDLIEEAI
                     GMYDALAMMKFSPGLLQREQLSAALDRLKRISDGLKRD"
     gene            1168..>2236
                     /locus_tag="VFU15_17320"
     CDS             1168..>2236
                     /locus_tag="VFU15_17320"
                     /inference="COORDINATES: protein motif:HMM:NF024962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719610.1"
                     /translation="MKRLLPLVFSVLAAGSAYAQHGATPESGHNPVDPLTTAHSTGPL
                     SPMDGNATLGPTYAFSACGLNYTTATQKIGQRFSPVGVPQPATFAIAGIPATAVIQQA
                     FVWCDASGNGIPITITVANPFTTSASFPMTMIGQDQDKCWGYQGTYSYRADVTSIIAG
                     NGNYTISGFPTGSTNDVDGATLMIIWSDPTASYQGDINIWDGCVVINGGTTTQTINNM
                     SACTGTIQSARAFTCIADLQGLGSQITLNGMPPIGVTEDWWNYVDVSTTVTPGQATST
                     FGNNSSGDCYNFCVMGLYWRSNCTTCCANPFTLNMTSSPSQCTSSNGTATATPVGGSG
                     PFSYTWNTPSSDSTQTANGLPP"
BASE COUNT          553 a          614 c          581 g          488 t
ORIGIN      
        1 cgtcggcgaa acgatgtaca aagaatttgt tttcccgaat acagcaggaa agctgatgct
       61 tccttcgtac gattacagtt gctcggtgaa gcagaatccc atgcccaccg gcaatgattt
      121 tatcgacgac atcatgagca tcgatatgcc cgtgacgctt cattccgagc ccgttgaaat
      181 aactgcgctc ccggttccgg atgcgaacaa gccggcggat tttctcggcg acgtcggtaa
      241 atacgcgctg agcgccagca tcgaccggca gaaagtgaaa accggcgaac ctgtaaagct
      301 caccgtcatg atcacgggaa gaggcaatgt cagcttcctg cgcctcggcg accagcattt
      361 tccgccgggc attgagccgt atcctgttgt caataacgac agtctcgtga tcaccggctc
      421 cggggtgacg ggaagcaaga attttacatt cacgctcatc ccgaaaaaag aaggtgatta
      481 tacgatcagc ggcatcacgt tttcctatta cgacgtttcg aaaaaagaat acgtcacgct
      541 gaagacgccg gatttttcat tgcacgcaga caaaaatgaa aatgaaaaaa cggaggagga
      601 gaacaacctt ccgaagggat tcccggttgc ggacaacagc gattttcttt tcggtctgaa
      661 ggtcgtcgcc ctgctgttcc ttatttccgg cggcatctgg tattattatg cgcgcaacaa
      721 aagggaagtg gaaaaacagc ggatggaatc tgaaaaaaaa caggtcccgg tggaagagcc
      781 ggtctttacg gtgacaaagc ctgtcgtgaa aaacaacctg gaaacggcat ctttcatgat
      841 cagctctgga aattattccg gggcggtgaa atgcatgtat gacgagcttg tttcttcact
      901 gtgccgctgc tgcgagtttt ctcccgaaga attttccgtc agccaggtgc gctaccgcat
      961 gcagatgaaa aaattcagca gcgatttgat cgaagaagcg atcgggatgt atgatgcgct
     1021 cgcgatgatg aaattttccc ccgggctcct gcaacgggaa cagctgagcg ctgcactcga
     1081 tcgtttaaag cgaatcagtg acggcctgaa aagagattag gttaattcat tgattttctt
     1141 aaatttgcgt ttagcaaacg cacccatttg aaacgtcttc ttccccttgt attttctgtt
     1201 cttgcagcag gatctgcata cgcccagcat ggcgccacac ctgaaagcgg tcacaaccct
     1261 gttgacccgt tgaccactgc tcattcaacc ggcccgcttt ccccgatgga cgggaatgcg
     1321 acgctgggac cgacgtatgc gttcagcgcg tgcggactca attacaccac ggccacgcag
     1381 aagatcgggc agcgtttttc cccggtaggc gttccccagc cggcgacatt cgccattgcg
     1441 ggaattccgg caacggccgt catccagcag gcattcgtct ggtgtgacgc ttcaggcaac
     1501 gggatcccga tcaccatcac cgtcgcgaat cctttcacca cttcggccag tttcccgatg
     1561 acgatgatcg ggcaggacca ggacaaatgc tggggctacc agggaacata ttcctatcgc
     1621 gcggacgtta cgtccatcat tgccggtaac ggaaactata ccatcagcgg tttcccgacg
     1681 ggaagcacca atgacgtgga cggcgcaacg ctgatgatca tctggagcga tcccaccgcg
     1741 agctaccagg gagatatcaa tatctgggac ggctgcgtgg tgatcaacgg tggtactacc
     1801 acgcaaacga tcaacaacat gtccgcatgt accggcacga tacaaagtgc acgcgcgttc
     1861 acgtgcatcg ccgacctgca gggcttaggc tcacagatca cgctgaacgg gatgcctccc
     1921 atcggtgtca cggaagactg gtggaattac gttgacgtat ctacgaccgt aacacccggc
     1981 caggcgacgt cgaccttcgg gaataattcc agcggtgatt gctataattt ctgcgtaatg
     2041 ggtttatact ggagatcgaa ctgcaccacc tgctgtgcaa acccgttcac gctcaatatg
     2101 acgtcatcgc cgagccagtg cacttcctcc aacggaacgg ccaccgcgac gcccgtcggc
     2161 ggatcaggtc cgttcagcta tacatggaac acgccttcgt ctgattccac gcagacggca
     2221 aacggacttc cgcccg
//
LOCUS       DASTSF010000616         2277 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1247452,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000616 DASTSF010000000
VERSION     DASTSF010000616.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2277
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1247452"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..626
                     /locus_tag="VFU15_17325"
     CDS             <1..626
                     /locus_tag="VFU15_17325"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEU4719611.1"
                     /translation="PNCSTIQMVVALNPLHKKYKAKRIVVSTYQSVTGTGKKAVDQLM
                     NERRGEKGEMAYAYTIDMNAIPQIDVFLENGYTKEEMKMVNETKKIMRDDSIRLTATT
                     VRIPVMGGHSEAVNVEFENEFDLAEVRSLLENAPGVAVVDDIKNLQYPMPLLHAHNRD
                     EVFVGRIRRDETQPRTLNMWVVSDNLRKGAATNAVQIAEYLVAHKLV"
     gene            complement(628..1425)
                     /locus_tag="VFU15_17330"
     CDS             complement(628..1425)
                     /locus_tag="VFU15_17330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719612.1"
                     /translation="MKKLFRFFSHPWAIFISNLLLAAGAYAGNEIFQAFCKPVTWASA
                     VLLFFITAFLAWPFVPEEKKQLKNFLAIFHGAGFCVCVYCTWFGKMMLVYSLFALVAG
                     VGIFGFAPVIFLVQIVYRFVKTQDTKRRWFFFAGVLPLIVFQLYFALQYRALENAITK
                     IPPQERTPEKLAGVLPRNYMAERFAGTYFRYHTSYCEYDGWRPPLHDPFLVISRRLFP
                     SVDNSVPGYFAERAALYRLMFPGKNAYAGCNCSDLNGDSKTYPENAP"
     gene            complement(1473..>2277)
                     /locus_tag="VFU15_17335"
     CDS             complement(1473..>2277)
                     /locus_tag="VFU15_17335"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:NF011369.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="lipoprotein signal peptidase"
                     /protein_id="HEU4719613.1"
                     /translation="TGSWFYLHFVENPGMAFGLQLGDGAGGKLLLSLFRLVAVGGIGY
                     YLWKKTKENVRPLLIVCVALIFAGALGNILDSVFYGKFFGASDPWDQNIAQFMPASGG
                     YAGFLHGQVVDMFYFPVLRGHFPQWFPLWGGEDYLFFRPVFNIADASISVGVFLLIVF
                     QARLFSKADRPVGEKRILLTNIFFSFLAFLISSFLLFTYMTLASKTHPLSGGRIALVF
                     GLAAVAGAGMFLFLKNYPVYVPPAPPAPATEAKESADHTSADSAPEENS"
BASE COUNT          606 a          616 c          651 g          404 t
ORIGIN      
        1 acccgaactg ctcgacgatc cagatggtcg tcgcgctgaa cccgctgcac aaaaaataca
       61 aagcgaagcg catcgtcgtc agcacgtacc agtcggtgac cggcaccggg aaaaaagcgg
      121 tcgaccagct catgaacgaa cgccgcggtg aaaaagggga aatggcttac gcctacacga
      181 tcgacatgaa cgccattccg cagatcgacg ttttccttga gaacgggtac acgaaggaag
      241 agatgaagat ggtgaacgaa acgaagaaga tcatgcgcga cgattccatc cgccttaccg
      301 caacgacggt ccgcattccc gtcatgggcg ggcattccga agcggtgaac gtggaattcg
      361 aaaatgaatt cgatctcgcg gaagtccgtt cattgctgga aaacgccccg ggagttgccg
      421 ttgtcgacga cataaaaaat ctccaatacc ccatgccgct ccttcacgcg cacaaccgtg
      481 acgaagtttt cgtcggccgc attcgtcgtg atgaaacaca accgcgaacg ctgaacatgt
      541 gggtggtttc cgacaacctc cgcaaaggag cagcgacgaa cgcagtgcag atcgccgagt
      601 atcttgtggc gcacaagctg gtgtagctca cggcgcattt tccgggtacg tcttcgaatc
      661 tccgttcagg tcggagcagt tgcaaccggc gtacgcgttt ttccccggaa acataaggcg
      721 atacaaggct gcgcgttcag cgaagtaacc cggaacggag ttatccaccg aagggaacag
      781 gcgcctgctg atcaccagga aaggatcgtg cagcggcgga cgccagccat cgtattcgca
      841 ataggaagta tgatagcgga aatacgttcc ggcaaaacgt tcggccatgt aattgcgggg
      901 taaaacgccg gccagctttt caggtgtgcg ttcctgcggc ggaattttcg tgatggcatt
      961 ttccagtgcg cggtattgca gcgcaaaata aagctggaaa acgatcagcg gaagaacgcc
     1021 cgcgaagaaa aaccacctgc gtttcgtgtc ttgggttttg acaaagcggt aaacgatctg
     1081 cacgagaaag atcacgggcg cgaacccgaa aattcccaca cccgcgacga gcgcgaacag
     1141 cgaatacacc agcatcattt tcccgaacca ggtgcaatac acgcatacgc agaacccggc
     1201 gccgtggaag atcgccagga aattcttcag ctgttttttt tcttccggca caaacggcca
     1261 ggcaagaaat gcggtaataa aaaacaggag cacggcagac gcccacgtca ccggtttgca
     1321 gaacgcctgg aagatctcat tgccggcgta agcgccggcg gcaagcagca ggttggaaat
     1381 gaagatcgcc cacgggtgcg aaaaaaagcg gaacagtttt ttcacccctg tttaacgcag
     1441 gaatgaaaac gaaattgccg cggcgggaat gttcaggaat tttcttcggg tgcggaatcc
     1501 gcagatgtgt gatccgcgct ttctttcgct tccgtcgcag gagccggggg agcaggcgga
     1561 acgtaaacgg ggtaattctt caggaacagg aacatgcccg ctcctgcaac agcggcaaga
     1621 ccgaacacga gagcgatcct tccgccggaa agcgggtgcg ttttcgacgc gagcgtcatg
     1681 taggtaaaaa gcaggaacga gctgatgagg aacgcgagga aggaaaagaa aatgttggtg
     1741 agaaggatcc gtttttcacc gaccgggcga tccgccttgc tgaaaagccg tgcctggaaa
     1801 acgatgagca ggaacacgcc gacggagatc gatgcatcgg caatattgaa aacaggacgg
     1861 aagaaaagat aatcctcacc gccccagagc gggaaccatt ggggaaaatg cccgcgcagc
     1921 acggggaaat agaacatgtc caccacttgt ccgtgcagga atcctgcgta cccgccgctg
     1981 gccggcatga actgcgcgat gttctggtcc cagggatcgc tggcgccgaa aaatttcccg
     2041 tagaaaacgc tgtcgagaat attgccgagc gcaccggcga agatgagcgc cacacacacg
     2101 atcagcaggg gccgcacatt ctctttcgtt tttttccaga ggtaataccc gataccgccg
     2161 acagccacga ggcggaacag gctgagcagg agttttcccc cggcgccgtc accgagctgc
     2221 aggccgaacg ccatgcccgg gttttccacg aaatgcaggt agaaccagct tcccgtc
//
LOCUS       DASTSF010000617         5023 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1176534,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000617 DASTSF010000000
VERSION     DASTSF010000617.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5023
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1176534"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            453..4577
                     /locus_tag="VFU15_17340"
     CDS             453..4577
                     /locus_tag="VFU15_17340"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEU4719614.1"
                     /translation="MKRILFILVFSGLHFSFLLADNYYWVGGTGNWSDYVHHWAVSSG
                     AQLYHDHVPTPQDTVIFDSLSFTQAGDTVYADTSIMYCHTMKWENVQFHPRYYCNVIY
                     TNSFLRIYGSLLLDTGMTWSYEGRLIFSGQQGNLHLRCAGQYLYSLEFSGDTASHWET
                     DDSLSAGSLHLFSGGFSSNGMPMNVSQLVLDNFGITGFDLDTSTLYVVSFESVVPPSL
                     VDADGSIIFAYGSPFRTAGISFGVVDFVHACYVTGGGNFRKAIFNSDATLASSFAFDT
                     LVVNGTGTKLVLQSGSVQVINDTMIDNGGCRGLNEISASVYGVAAQLSKAAGSITMDR
                     MFLENVDATGGAAFTASNSVLMNSSGWTSVSTPAPRNLWWTGNSGTWSDTAHWASSPG
                     GPGGECAPTPLDSVFVDTSVPANDTIVTDYAHIYCGNFDWSTGGSFLQQSVELEDFGS
                     MQLDSAMSFYSPAVNFRSPQMGNVISPAGKAFGIMSFTSPGSWDLTGRLSASEMNIAG
                     GSFSSNANVITAGTISIGGNPSSVISFGGSYVLASRWENYSPVPVFTAPDTLITNELT
                     DMSANYYPVVIFPNGGSLSANSHLHKTSFGGNTFISGNNLFDSLLIAGQGEEISFESG
                     STQLLGYFDVQSSCTGMSWIHGSVTGQAASLNSISGTMMLHDVILDDLSFSGSCQALN
                     SIANSNVNGITVTPPPSGPMYWINGTGNWNDPMHWSPVSGGAPGTCVPNPLTDVFFDA
                     NSFTSPSDAVQLNVPVAYCHDMTWSNCAGAPAFTDSWGNGLLKCYGGFSLDTNVTADN
                     VSIHFRAGTGNHVINTNGQPLGNLYFEGIGGSWSLQSDLSASAMRLNYGNFYTNSHTL
                     QLGNFSSDMLSLRSLHLDTSLVYCGNWEAGNDSLFTLDADSSVFVITGDTFRGGNRQY
                     YPDVTFAGDVNVSGSDTIGIARFTGMTALYSAVTFDTLLLDNPGSQLTIGPGLTETVR
                     GKMLANSSASKLIGVFSGGAGFASLQKTADTVCLNFLVLQGIHAVGGATFYAGTYSTD
                     IGNNSGWTFQDCNPQTVDVWPGDANRDLTADNLDLLAIGVAFGQSKTPRSNASNNWTA
                     QPSWIWQVLFANGTDITNADCDGDGTVGYSDTTALVLNYGLTHPARYSAPDSTQVTGP
                     KLWMLTPAYVNAGDTGSIAIMLGDSTMPANGVYGISFQLNYNDSDIIAPGSVWIEFDN
                     SWMAPTGSVVHLLKSFTGQSIMEVAISRIDHHDVSGSGMIARIHFTANANYFGLFKGW
                     FTDMHMINSEEVPLPVSMQLGTFYITGMQDYSSAGFHGYPNPTDGNYFIDDPSLAGTS
                     SVITVTDLQGRTLYEEKSRNETFIRIDLSALPAGTYFISVTNENGTFIQRVIRQ"
     gene            complement(4626..>5023)
                     /locus_tag="VFU15_17345"
     CDS             complement(4626..>5023)
                     /locus_tag="VFU15_17345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719615.1"
                     /translation="NMPFNDRYRFTEALMYYAFNHPGNVYHTRGDSVDFTRPFKSADD
                     LNGQFVQWDSTVTIDDPNNPGSFITAPIKFEKTIADIYAIRFYEDWYYDPKTMMMTKI
                     VHGVSLILITQDYNGNPLLRDAGIYIKTD"
BASE COUNT         1132 a         1395 c         1300 g         1196 t
ORIGIN      
        1 ccccgatgaa atgattgagg ttgccgacca ggtgcaacgt aaggttgcct gcgctgttcg
       61 tgatgtcgcc ggtgatcttc cagagatcat catcgctgtg gaattttttc agctccgctt
      121 caagctgatc aataccgtga agaaaaaagt gacggaaatg cgaaacctgg ctcatgcgtt
      181 ctttttttca aaggtaaaca gtacctgctg gaaatgcagc ttcatcgccg ttcaatattt
      241 tgccgctcaa ttatttgcaa cagcaggccc acatcttgac gtgtaaaatg ctgtcgaaga
      301 ttggcgtgcg tccggatttc ctatttgtgc tttcccaaat cttttgtttt cagcagttta
      361 ccggcctgtt tttctcttga catgtataat catcgcggat tcctgtcttg aacggcgtct
      421 tgcttaaaag ttgctttgta aaaaagaaga tcatgaaacg catcctgttt atcctcgttt
      481 tttccggcct gcatttttcc ttcctcctcg ctgataatta ttactgggtg ggcggaaccg
      541 ggaactggtc cgactatgtt catcactggg ccgtcagcag cggagcacag ctgtaccacg
      601 atcacgttcc cacgccgcag gacacggtta ttttcgattc gctttctttc acacaggccg
      661 gtgatacggt ttacgcggat acatcgatca tgtattgcca taccatgaaa tgggagaatg
      721 tacagttcca tccgcggtat tactgcaacg ttatctacac gaactccttc ctgcgcatat
      781 acggatcgct gctgctcgat acgggcatga catggagtta cgaaggccgg cttatcttca
      841 gcggacagca gggcaatctt catcttcgct gcgcaggaca gtacttatac tcactggaat
      901 tctcgggcga cacagcttcg cactgggaaa cggatgattc gctttccgcc ggcagcctcc
      961 atttgttttc tggcggattc agcagcaacg ggatgccgat gaacgtctcg cagctcgtac
     1021 tcgacaactt cggcatcacc ggcttcgacc tcgacacctc gacgctctat gtcgtgtcat
     1081 ttgaaagcgt ggtccctccc tcactggtcg atgcggacgg ctccatcatt ttcgcttacg
     1141 gatcgccttt ccgaactgcc ggcatcagtt tcggcgtcgt ggattttgtt cacgcctgtt
     1201 acgtaaccgg aggcggaaat ttccgcaaag ccattttcaa ttccgatgcg acgctcgcca
     1261 gttcgttcgc attcgacacg ctggtggtga acggaaccgg aacaaagctc gtgctgcaaa
     1321 gcggttccgt gcaggtcatt aatgacacga tgatcgacaa tggcggctgc cgcgggctca
     1381 atgaaatttc agcatccgtt tacggcgttg ccgcacagct cagcaaagcc gcgggatcaa
     1441 taacgatgga ccgcatgttc ctggagaacg tagacgcaac gggcggagcg gcattcaccg
     1501 cctcgaattc cgtgctgatg aacagcagcg gctggaccag cgtaagcaca ccggcgccgc
     1561 gcaatctctg gtggacaggc aacagcggca catggagcga cacggcacac tgggcttcat
     1621 cgcccggcgg accgggagga gaatgcgccc ccacaccgct cgacagtgtt ttcgtggata
     1681 catccgttcc tgcgaacgat acgatcgtta cggattatgc gcacatctac tgcgggaatt
     1741 tcgactggag cacaggagga agcttcctcc aacaatccgt cgaactggaa gatttcggtt
     1801 ccatgcagct cgacagcgcc atgtcattct attcacccgc cgtcaatttc cgttcacccc
     1861 aaatgggaaa tgtcatttca ccggcaggaa aagcattcgg catcatgtcc tttacttcgc
     1921 cggggagctg ggatcttacc ggccgcctgt ccgcatcaga aatgaatatc gcaggcggat
     1981 ctttcagcag caatgcgaat gtcatcacag cgggaaccat aagcatcggg ggaaatcctt
     2041 cttccgtcat cagcttcggc ggaagctacg tgctcgcatc gcggtgggaa aattattcac
     2101 ctgttcctgt tttcactgca cccgatacgc ttatcacgaa tgagctcacc gacatgtcgg
     2161 cgaattatta cccggtcgtc atttttccga acggcggttc gctttcagcc aacagtcatt
     2221 tacataaaac ttcattcggc ggaaacactt tcatcagcgg gaataacctg tttgattcgc
     2281 tgctgatcgc gggacaggga gaagagatct ctttcgaaag cggttccacg cagctgctcg
     2341 gctatttcga cgtccagtct tcctgcaccg gcatgagctg gatccacggc tcggtgacgg
     2401 gacaggccgc ttcgctgaac agtatttccg ggacgatgat gctgcacgat gtgatcctgg
     2461 acgatctttc attttccgga tcgtgccagg cgttgaattc gatcgcgaac tcgaacgtga
     2521 acggaattac cgttacaccg cctccgtccg ggccaatgta ctggatcaac ggcacaggca
     2581 actggaacga cccgatgcac tggtcgccgg taagcggcgg cgcacccgga acgtgcgttc
     2641 ccaacccgct cacggatgtt ttcttcgatg cgaattcatt cacatcaccg tccgacgccg
     2701 tccagctgaa tgttcctgtc gcgtattgcc acgacatgac ctggagcaac tgcgccggcg
     2761 cacccgcctt cactgacagc tggggcaatg gcctgctgaa atgctacgga ggattttcgc
     2821 tggacaccaa tgtcaccgcg gataacgttt cgattcattt ccgcgccggg acaggaaacc
     2881 acgtcatcaa cacgaacggt cagccgctcg gcaatctgta tttcgaaggc ataggcggat
     2941 cgtggagcct ccaatcggac ctgagcgcat ccgccatgag gctgaactac ggaaatttct
     3001 atacgaacag tcatacgctg cagctgggca atttctccag tgatatgctt tcgctccgct
     3061 cgcttcacct cgacacatcg ctcgtgtatt gcgggaactg ggaagccggg aacgacagcc
     3121 tgttcacact cgatgcggac agcagcgttt tcgtcatcac cggcgataca ttcaggggcg
     3181 gaaaccggca gtattacccg gatgtcacgt tcgccggcga tgtgaatgtt tcgggcagcg
     3241 acacgatcgg gattgcaagg tttaccggga tgacggcgct ttattcggcc gtgacattcg
     3301 atacgcttct cctcgataat cccggcagcc agctgacgat cggtccggga ctcaccgaaa
     3361 cagtccgcgg aaaaatgctt gccaattcca gcgcgtcgaa actcatcggt gttttctccg
     3421 gcggcgctgg tttcgcttcc ctgcagaaaa ccgccgatac ggtctgcctt aattttctcg
     3481 tgctgcaggg aattcatgca gtcggcggcg cgacatttta tgcaggtaca tacagcacgg
     3541 atatcgggaa taacagcggc tggactttcc aggattgtaa tccgcagacc gtcgacgtat
     3601 ggcccggcga tgcgaaccgt gatcttaccg cggacaatct cgatcttctc gccatcggcg
     3661 tcgcattcgg tcaaagcaaa acaccgcgca gcaacgcatc caacaactgg acagcacagc
     3721 cctcctggat atggcaggtg cttttcgcga acggcaccga catcaccaac gccgattgcg
     3781 acggtgacgg aacagtcggc tacagcgata caactgcgct cgtgctgaat tacgggctga
     3841 cgcatcctgc ccgctattcc gcacccgatt ccacgcaggt gacgggtccg aaattgtgga
     3901 tgctcacacc ggcgtatgtg aacgccggcg atacgggaag cattgcgatc atgctcggcg
     3961 attccacgat gccggcgaac ggtgtgtacg gcatttcatt ccagctcaac tacaacgatt
     4021 cggacatcat cgctcccggt tccgtgtgga tcgaattcga caactcatgg atggctccga
     4081 cgggaagtgt cgttcacctg ctgaaaagct tcaccggtca gagcattatg gaagtcgcca
     4141 tcagcaggat cgatcaccac gatgtttcgg gaagcgggat gatcgcgcgg atccatttca
     4201 ccgcgaacgc aaattatttc ggacttttca aaggctggtt cacggacatg cacatgatca
     4261 acagcgagga agttccgctg cccgtttcca tgcagctcgg gacgttttac ataacaggca
     4321 tgcaggatta ttcttccgcc gggttccacg gctatcccaa tccgacggac ggaaattatt
     4381 tcatcgatga tccttccctg gccggcactt catcggtgat cactgtgacg gatctgcagg
     4441 gaagaacatt gtatgaagaa aaaagcagga acgaaacctt catccgcatc gatctttccg
     4501 cgcttcccgc cgggacctat ttcatctccg ttaccaatga gaacggaact ttcatacagc
     4561 gggtgatacg gcagtaagaa aaatcagccg gttttcattt tgagaatctc cgggaacggg
     4621 gattctcaat ctgtttttat ataaatgccg gcatcccgca ggagcgggtt cccgttataa
     4681 tcctgcgtga taagaatgag gcttacgccg tgcacgatct tggtcatcat catggttttc
     4741 ggatcgtagt accagtcttc gtaaaaacgg atcgcgtaaa tatccgcaat cgtcttttcg
     4801 aattttatgg gcgctgtgat gaacgacccc gggttgttgg gatcgtcgat agtgaccgtg
     4861 gaatcccact gcacgaattg tccattcaga tcatcggccg acttgaaagg acgcgtgaaa
     4921 tccacactgt cgccacgcgt gtgatagacg ttccccgggt gattgaacgc ataatacata
     4981 agcgcttccg taaagcggta gcgatcgttg aacggcatat tgc
//
LOCUS       DASTSF010000618         5655 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_672115,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000618 DASTSF010000000
VERSION     DASTSF010000618.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5655
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_672115"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1076
                     /locus_tag="VFU15_17350"
     CDS             <1..1076
                     /locus_tag="VFU15_17350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719616.1"
                     /translation="GKVAWMGNPFGVDDLSFSARYDESGKAFVIYGVNTDSLLNVLVM
                     AFRDDFEHPLYSGSYSVAHPEHEEPNDHSYFFMHDGRLNVASDYQVDSVSIDSVSKSF
                     TYRPWKFADSSFFILPGHVVELDNFRDRNDTLRDNSPRVCCRVGTTGFTQNAHRYVPF
                     SMEEVCSLDYPVAGSSANSIFQAMRYSRDGGLSGLYYELPGWLVSLKDTALRFHNMLN
                     CSALEVSEPSGPRKFHFTIPDVFFLELKNFLVHRDTLYVFSDVMDEYAAFNLRTGKRI
                     DDPPVLKIIDKRFMLASSAWFYSAWILCLAFIPAFLLFLLVFRLVKKRKYTGWFSVQR
                     AFVLWFFPSLYGLYWIFYCFFRI"
     gene            1094..1447
                     /locus_tag="VFU15_17355"
     CDS             1094..1447
                     /locus_tag="VFU15_17355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719617.1"
                     /translation="MEIAGKEEKTPLKKFLLRTVLGNSVLTGCFVAALLTCNMRYTSG
                     TATRETFLLNLVSLLMCSVHTVLVLIFSLRRFAKKETLNGIILLAYFFLSSAIVYFLF
                     FLLSFFGFFFSMKGH"
     gene            1613..2848
                     /gene="rocD"
                     /locus_tag="VFU15_17360"
     CDS             1613..2848
                     /gene="rocD"
                     /locus_tag="VFU15_17360"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002693739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004587 - ornithine aminotransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HEU4719618.1"
                     /translation="MTTVADKLTRSEELMSMEARYGAHNYHPLPVVLEKGEGVFVWDT
                     EGKKYYDFLAAYSAVNQGHCHPRIIAALTEQAKRLTLTSRAFFNDSLGEYEKFITEFF
                     GYDKVLPMNTGVEAVETAVKLARRWGYKVKGIPGNQAKIVWAENNFHGRTISAISAST
                     DPSSYGGFGPYVPGYVIVPYNDLAALENALKDPHVAAFIFEPVQGEAGVVVPDEGYLS
                     GVREMCSRHNVLMVADEVQSGLGRTGKMLACDHENVRPDLLVLGKALSGGVLPVSAVL
                     ADDAVMLTINPGEHGSTYGGNPLACKVAMAALNVLRDEKLVENSERLGRILRAELKKI
                     ASPRISLIRGKGLFNAMVIKPEKDVTAWDVCLKMMENGLLAKPTHGDIIRFAPPLCIT
                     EKQLSECVDIIRESILSFD"
     gene            2871..3284
                     /locus_tag="VFU15_17365"
     CDS             2871..3284
                     /locus_tag="VFU15_17365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719619.1"
                     /translation="MKFLFSRRRLILGIAAAGNFIGSIAAIISPAFFFSQFFRYQPDR
                     YATFPWLAMYHYTFWAFVLIMGIAFLAGAIDPVKNRIVLMIGGFGKVVAAAFWVMLYS
                     QGHGRWLMISGGIWDTVLGIIMLMLFFVKTEEKAS"
     gene            complement(3323..4231)
                     /gene="xerD"
                     /locus_tag="VFU15_17370"
     CDS             complement(3323..4231)
                     /gene="xerD"
                     /locus_tag="VFU15_17370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011586063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEU4719620.1"
                     /translation="MAWKSEIAGFRTYLRLEKSLSPNSVESYVHDIEKLVQYLEYSGT
                     PLSAAQIDAKHIRGFLNWISELGMTATSQARILSGIRAFYHFMLMENMLTADPTELIE
                     GPKTMRKLPDTLSNDDIEKLLASIDRSTPEGERNVAIIETLYSCGLRVSELVNLRISD
                     LFTGEGYIRVIGKGNKERLVPIGGMAVKQIRNYMENVRVHIAIKKGQEDFLFLSKRGT
                     KLTRVMIFTMVKTLAEKCGLRKKISPHTFRHSFATELVEGGADLRAVQEMLGHSSITT
                     TEIYTHIDREFLRSQVKRFHPRSRKS"
     gene            4305..4727
                     /gene="aroQ"
                     /locus_tag="VFU15_17375"
     CDS             4305..4727
                     /gene="aroQ"
                     /locus_tag="VFU15_17375"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008612913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HEU4719621.1"
                     /translation="MRILILNGPNLNLLGKREPDIYGGNSFETYLSFLKDEFPHISFD
                     FYQSNVEGELINKLHECGFSHDGIVFNPGGYTHTSVSIGDAVAAIQAPVVEVHISKVL
                     AREDFRHHSFVAPHCRGSISGFGLDGYRLAVLSFLAPE"
     gene            4773..>5655
                     /locus_tag="VFU15_17380"
     CDS             4773..>5655
                     /locus_tag="VFU15_17380"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU4719622.1"
                     /translation="MDIPPGNLQRVVIVGGGFAGIELAKQLHNRFQVVLIDKNNFHPF
                     QPLLYQVATAGVEPDSVVFPLRKIFSGYREFYFRLTEAQEILPGKNILVTPIGDLQYD
                     YLVLATGSDTNFFGMKETQKFSLAMKTVREALDLRSCILQNFEMALIVPDMEKKQALM
                     NYVIVGGGPTGVELAGALAELRKHVLPNDYPELDLMKMEIHLVDTGARLLGTMDERSS
                     LKAEKFLRDMGVDVLLNTRITGYDGKTATTDTGKMFSCATLIWAAGVKGVPLKGIPEN
                     ALLPNGRIKVDAFGKVNG"
BASE COUNT         1205 a         1493 c         1457 g         1500 t
ORIGIN      
        1 atggcaaggt ggcttggatg ggaaacccgt ttggcgtgga cgatctttcg ttttcggcgc
       61 gttacgatga gagcggcaaa gcgtttgtta tttatggcgt gaacacggat tcgctgctga
      121 acgtgctggt aatggctttc cgcgatgatt ttgaacaccc gctgtattcc gggagctatt
      181 ccgtggcgca tccggagcac gaggaaccca atgaccattc gtattttttc atgcacgatg
      241 gccggctgaa cgtggcatcg gactaccagg ttgattcagt cagtattgat tccgtttcaa
      301 aatccttcac gtatcgacca tggaaatttg cagacagcag cttttttatc ctgccggggc
      361 acgtggtgga actggataat ttcagggaca ggaacgatac tcttcgcgat aattctccgc
      421 gcgtttgctg tcgcgtcggt accaccggct ttacgcagaa cgctcaccgg tacgttccgt
      481 tttcaatgga ggaagtgtgt tccctggatt atcccgtcgc aggttcttcc gcgaattcca
      541 ttttccaggc gatgaggtac agcagggacg gaggactttc cgggttgtac tatgagcttc
      601 cgggatggct ggtttccctg aaggatacag cgttgcgttt tcacaacatg ctcaattgtt
      661 ccgcgctgga ggtttccgaa cccagcggcc cgcggaaatt tcacttcacg atcccggatg
      721 tgttttttct tgaactgaaa aattttctcg tgcaccgaga tacgctgtat gtgttttcag
      781 acgtcatgga tgaatatgcg gcgttcaacc tgcgtaccgg taagcgtatc gatgatccac
      841 cggtgctgaa aataattgat aagcgattca tgctcgcatc ctccgcatgg ttttattccg
      901 cctggatact ttgcctggct tttattcctg cgttccttct tttcctgctt gtttttcgtc
      961 ttgtaaaaaa gagaaaatac accggatggt tttccgttca gcgcgcgttc gttctctggt
     1021 tcttcccctc gctgtacggg ctttactgga tcttctattg tttcttccgg atctgattta
     1081 gtatatttca aaaatggaaa tcgccggaaa ggaagaaaaa acgccgctga agaaattttt
     1141 gctgcgaaca gtgctcggca acagcgtgct taccggctgc tttgtcgctg cgctgctcac
     1201 ctgcaacatg cgctacactt ccggaaccgc cacgcgcgaa acattcctgc tgaatctcgt
     1261 ttccctgctc atgtgcagcg ttcataccgt gctggtgctg atcttttccc tgcgccgctt
     1321 cgctaaaaaa gaaacgctca acgggatcat cctgctcgcg tatttctttc tttcgtccgc
     1381 gatcgtttat tttctttttt tcctgctctc gtttttcggg ttcttctttt cgatgaaagg
     1441 acattgatcg ttttccctgc aaactttttc cggcttacgg atctttttcc tgtttaaact
     1501 ccaggccttc catttaaccg gattcttgcg cacctcacat tcccgcgcat taccccatcc
     1561 cgttttcgta tatttgcatt ctcaatcaac agaataccaa tccctgaatt tcatgaccac
     1621 cgtggctgac aaacttaccc gttccgaaga gctgatgtcg atggaagcgc gttatggcgc
     1681 tcataattat catccgcttc ctgttgtgct ggaaaagggg gaaggtgttt ttgtctggga
     1741 cacggaggga aaaaaatatt acgattttct tgccgcttat tcagcggtca accaggggca
     1801 ctgtcatccg cgcatcattg ccgcgcttac ggaacaggcg aagcgcctta cgcttacctc
     1861 gcgcgctttt ttcaatgatt ccctcggtga atatgaaaaa ttcatcacgg aatttttcgg
     1921 gtacgacaaa gtgctcccga tgaataccgg cgtggaagcc gtggaaactg ctgtgaagct
     1981 ggcccgccgc tggggataca aagtgaaagg cattcccggt aaccaggcga agatcgtctg
     2041 ggcggaaaat aattttcacg gccgcacgat ctctgccatt tccgcgtcta ccgatccttc
     2101 ttcttacggt ggcttcggtc cctacgttcc gggttatgtc attgttccgt acaacgatct
     2161 cgctgcgctc gaaaatgcgc tgaaagatcc gcatgtggct gcctttattt tcgaacctgt
     2221 tcagggagaa gccggggtag tggtgcccga tgaaggttat ctttccggcg ttcgtgaaat
     2281 gtgttcgcgt cacaatgtcc tgatggttgc cgatgaagtg cagtcgggac tcgggcgcac
     2341 gggcaaaatg cttgcctgcg atcacgaaaa tgtgcgcccg gatctgcttg tgctcggcaa
     2401 agcgctttcg ggtggtgtgc ttcccgtgag cgcagtgctc gccgatgatg ccgttatgct
     2461 cacgatcaac ccgggcgagc acggttcgac ctatggcgga aacccgctgg cctgcaaagt
     2521 ggcgatggcg gcgctgaatg tgctgcgcga tgaaaagctg gttgaaaact cggaacgtct
     2581 cgggcgcatt ctccgtgcgg agctgaaaaa aatagcttct ccccgcattt cacttatccg
     2641 cggaaaagga ctgttcaacg ccatggtcat caaaccggaa aaggacgtca cggcgtggga
     2701 tgtttgcctg aaaatgatgg agaacgggct ccttgcaaaa ccaacgcacg gtgatattat
     2761 ccgtttcgcc ccgccgctct gcatcactga aaaacagctt tccgagtgcg tggatatcat
     2821 ccgcgaatct attctcagct tcgactgatc tttctccaaa aaaatcttct gtgaagttcc
     2881 tgttctcccg tcgccgtctg atcctgggca ttgccgcagc cgggaatttt atcggctcca
     2941 tcgccgcgat catttcgccg gcatttttct tttcgcagtt tttccgctac cagcctgatc
     3001 gctatgcgac attcccctgg ctggccatgt atcactacac gttctgggct ttcgtgctca
     3061 tcatgggaat cgcttttctt gcaggcgcga tagatcccgt gaaaaacagg atcgtgctga
     3121 tgatcggcgg tttcgggaaa gtggtggccg ccgctttctg ggtcatgctt tattcgcagg
     3181 gacacggacg ctggctgatg atctccgggg gaatctggga taccgtcctt ggaattatca
     3241 tgctcatgct tttcttcgta aaaacagaag agaaagcttc gtaatagttt tcgttttgtg
     3301 gcgcggattt cccggcggta aatcaggatt tcctgctcct cgggtgaaac ctcttcacct
     3361 ggctgcgcaa aaattcccgg tcgatgtgcg tgtagatctc ggtcgtcgtg atcgaggaat
     3421 gcccgagcat ttcctgcacc gcgcggaggt cggcacctcc ttcaaccagc tcggtggcga
     3481 aggaatgacg gaacgtgtgc ggactgatct ttttccggag cccgcatttt tcagcaagtg
     3541 tttttaccat agtgaagatc atcacgcgcg tgagctttgt tccccgcttg ctgagaaaaa
     3601 gaaaatcttc ctggcctttt tttatcgcaa tatgcacgcg gacgttttcc atgtagttcc
     3661 ggatctgctt cacggccatt ccgccgatgg gaacgaggcg ttccttgttc cctttcccga
     3721 tcacgcggat gtagccttca ccggtgaaca ggtcggaaat gcggaggttc accagctcgg
     3781 aaacacgcag cccgcagctg tacagcgttt caatgatcgc cacgtttcgt tcgccttccg
     3841 gcgtgctgcg gtcgatggac gcaaggagct tttcaatgtc gtcattgctg agcgtgtcgg
     3901 gaagcttgcg catggtcttc ggtccttcga tcagttccgt cggatccgct gtgagcatat
     3961 tttccatcag catgaaatga tagaacgcac ggatgccgga aagtatcctc gcctgtgatg
     4021 tggcggtcat tcccagctcg ctgatccagt tgagaaaccc gcggatgtgc ttcgcgtcga
     4081 tctgtgcggc ggaaagcggc gtgcccgaat attcgaggta ttgcaccagc ttttcaatgt
     4141 cgtgcacata gctctctacg gagttgggcg acagcgattt ttccagcctg aggtaagttc
     4201 tgaatcccgc tatttccgat ttccacgcca tgcacaatga aagtttattg ctgaaaaata
     4261 aagaatcagc actgtaaaat aagaactaac gattaaattt gagaatgcgc atcctcatac
     4321 tgaacggccc gaacctgaac ctgctgggca aacgcgaacc ggatatttac ggaggaaatt
     4381 cattcgaaac gtatctttct ttcctgaaag acgaattccc gcacatttcc ttcgactttt
     4441 accagagcaa cgtggaaggc gagctcatca acaagctgca cgaatgcggc ttttcacacg
     4501 acggcatcgt gttcaatcca ggcggctaca cgcacaccag cgtttccatc ggtgatgccg
     4561 tggctgcaat tcaggcgccc gtggtggaag tgcatatttc caaagtgctg gcccgcgaag
     4621 atttccgtca tcattccttt gttgcgccgc attgccgcgg gagcatttcc ggtttcggcc
     4681 tcgacggtta ccgcctcgcc gtgctttctt ttcttgcacc ggaataaatc tttcccgatc
     4741 catcttatcg gtaactttgt gccgctatgt caatggatat tccccccgga aatctgcagc
     4801 gcgtggtcat cgtcggcggc ggttttgccg gtattgagct ggcgaagcaa ctgcacaacc
     4861 gtttccaggt ggtgcttatc gacaagaata atttccaccc gttccagccg cttctttacc
     4921 aggtagccac tgcaggcgtg gaacccgatt ccgtcgtttt tccgctgcgg aaaatattca
     4981 gcggctaccg cgaattctat ttccgcctta ccgaagcgca ggaaattctt cccgggaaaa
     5041 atattcttgt caccccgatc ggcgacctgc agtatgatta cctcgttctt gccaccggtt
     5101 ccgatacgaa ctttttcggc atgaaggaaa cgcagaaatt cagcctcgcg atgaaaaccg
     5161 tccgtgaagc gctcgacctc cgcagctgca tcctgcagaa tttcgaaatg gcgctcatcg
     5221 tgcccgatat ggaaaaaaaa caggcgctga tgaattatgt catcgtcggc ggcggaccta
     5281 caggggtgga gctcgcaggc gcgctggcgg agctgagaaa gcacgttctc ccgaacgatt
     5341 atcccgaact cgacctgatg aagatggaga tacatcttgt cgacaccggc gcccgcctgc
     5401 ttggcaccat ggacgaacgc tcttccctga aagcggaaaa attcctccgc gacatgggcg
     5461 tcgacgtgct gctcaacacg cggatcaccg gctacgacgg gaaaacggcg acaacggata
     5521 caggtaaaat gttcagctgc gccacgctca tctgggcggc aggtgtgaaa ggcgttccgc
     5581 tgaagggaat cccggaaaac gcattgcttc cgaacggaag aataaaagtg gatgcgtttg
     5641 gaaaagtgaa cggaa
//
LOCUS       DASTSF010000619         5592 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1918552,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000619 DASTSF010000000
VERSION     DASTSF010000619.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5592
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1918552"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..256
                     /locus_tag="VFU15_17385"
     CDS             <1..256
                     /locus_tag="VFU15_17385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719623.1"
                     /translation="GNIPHPSLRISVFHMNDKFIVKFEAGPMEQVYKFGQDKVSGMAD
                     IEKIVDETFITNVTKRFRDMIGDMQDAWERRQQAAGQKDV"
     gene            complement(253..1101)
                     /locus_tag="VFU15_17390"
     CDS             complement(253..1101)
                     /locus_tag="VFU15_17390"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEU4719624.1"
                     /translation="MTRIIRDVLRMENREPVAVDFHLPPLGKSPLPVIVFVHGFKGFK
                     DWGHWPLVAEHLSRAGFAVVRFNFSHNGTSPENQVEFVRPDLFGKNTFSKELDDVSLV
                     IDSLSWHVGHDSVFDPSRLGLLGHSRGGGIALLAAAQDKRVKAVATWAGVSDFEPRVN
                     PPETEKWKREGVRFVENTRTGQQLPVNYSFREDFYANRNRLDIKRSVAGMEVPMLVVH
                     GTADRTVPFREAEQLHSWNMFSRFVAVEEGDHTFGGRHPWESRELPALTVKALQPTIA
                     FFRENL"
     gene            complement(1098..1892)
                     /locus_tag="VFU15_17395"
     CDS             complement(1098..1892)
                     /locus_tag="VFU15_17395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719625.1"
                     /translation="MRASPGISMKKYFPFFFAAFVFTSCLDGIPISPDDTGSAQAADT
                     AKKDMPGPVPKEVRKGKVSSWGFDPDSCVNDLVLGSRSSFEKWWQKCGERSQRSDNNI
                     YSSAGHIVRHHAQGPPGEKIGENTYGVSWLNPSGNEQLDVYVYDDENGNETPYMLALQ
                     RPGSGSVIMPQGDTVYARDRHFMTGYGIYLGMSSYFVQSVYTDQPMMEWMTGDTLYLQ
                     YHPQQKDAKYYHYYSWKDYTATYKFTDDRLCRIEYYVSPSAFRKPE"
     gene            2026..3291
                     /locus_tag="VFU15_17400"
     CDS             2026..3291
                     /locus_tag="VFU15_17400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016550681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU4719626.1"
                     /translation="MAHPSQKQVHDPYASLRIRDFSIYLAARFFMTLGVQIQGVVVGI
                     QVYKLTHSAFQLGLIGLAEAIPFIGLSFFSGYVADIIRRKKIILASTTFLLLASWSLF
                     FVSHNVALLHELGAWPIFGIIFCTGIARGFLGPVFPAFLSQLVPRKLYPNATTWQSNL
                     WQTAAVLGPAMGGMLIGWESVIVAYGTCSSLITLSFFLMMLVTNHPLPPIEKKESLVK
                     SLTAGFRFVFSNQVMLAAFSLDLFAVLFGGAVAMIPVFANEVLHAGKDADSIIGWLRA
                     APAIGAIVSGFLLARFPPTKHAGRNLFIAVIGFGCCIIGFALSDIFLLSFIFLLISGA
                     FDQVSVVVRQSILQLLAPDNMRGRIAAVNGIFIGSSNEIGEFESGVTAKMMGNIPSVI
                     FGGCMTIAIVIAVMFSAPKLRNLDLAKIT"
     gene            3573..4607
                     /locus_tag="VFU15_17405"
     CDS             3573..4607
                     /locus_tag="VFU15_17405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719627.1"
                     /translation="MKSQTNPKTGEPRTVQAEFISRLKTVIPPNHSLVDELADILQVS
                     TDSAYRRIRGETALTIDEVSRLCSHYRIPFDHGAVKNGKASVTFSYHHLSDNSSNFID
                     YLNSVVSDLKRIKATEPNEIIFAAEDIPIFHHFRFPLLTAFKLFYWSKSILNVPEMEE
                     LRFDPASVPEKPVKLVREIYSLYQTIPSIEIWTEETLDSTLKQVEYYYESGLFQSKEQ
                     AIELLAEIGKMIAGVEKQAALSMKFTGDVPDANSNVNYSLYNSDLMMGNNCILVTAGG
                     ARFTYLVHNTFNSMLTTSQSFNEETERWLRNLIRKSNPLSGVSEKQRFRFFKKLNDKI
                     ARTREGISAG"
     gene            4609..5394
                     /locus_tag="VFU15_17410"
     CDS             4609..5394
                     /locus_tag="VFU15_17410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007094113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02757 family protein"
                     /protein_id="HEU4719628.1"
                     /translation="MAPGELRAFLELKTGEYNRPSFIASDPVSIPHLFSRKEDIEISA
                     FITATIAWGQRPVIIRNAKRFMELMEMAPYEFVLTASEKERARLGKFVHRTFNGIDAV
                     YFPGALRNIYAKHGGMENVFSEGLLRAENDFAMSAAIAHFRNVFFSLHHQKRTEKHVS
                     DPVSGSNAKRINMFLRWMVRKDNAGVDFGIWKSISPSLLLCPLDVHVGNTARALGLLK
                     RKQDDRKAVEELTGSLRLLDPHDPVKYDFALFGLGVFEKFGTR"
     gene            complement(5404..>5592)
                     /locus_tag="VFU15_17415"
     CDS             complement(5404..>5592)
                     /locus_tag="VFU15_17415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719629.1"
                     /translation="KEGLKLWKMQGDGIRFLPAVYPPSGRGFASIIFFRIPEQIRDPR
                     ALFFRRNEGFTLHAEKTR"
BASE COUNT         1245 a         1542 c         1452 g         1353 t
ORIGIN      
        1 cggaaacata ccgcatcctt ccctgcgcat cagcgttttt cacatgaacg ataaattcat
       61 cgtgaagttc gaggccgggc cgatggagca ggtttacaaa ttcgggcagg ataaagtttc
      121 cgggatggca gacattgaaa agatcgtcga tgaaactttc atcaccaatg tcacgaagcg
      181 tttccgcgac atgatcgggg atatgcagga tgcctgggag cgcaggcagc aggccgccgg
      241 tcaaaaagac gtttagaggt tttccctgaa aaatgcgatc gtgggttgaa gcgctttgac
      301 ggtgagcgca ggcaattcac ggctttccca cgggtgcctt ccgccgaacg tatgatcgcc
      361 ttcttccacg gcgacaaaac gcgagaacat attccaggaa tgaagctgtt ccgcttcgcg
      421 gaaaggaact gtcctgtcgg ctgttccatg cacgacaagc atcggcactt ccattcctgc
      481 aacggatctt tttatgtcga gacgattccg gttggcataa aaatcttccc ggaacgaata
      541 attgacaggc agctgctgtc ctgtcctcgt gttttcgacg aagcgaacgc cttccctttt
      601 ccatttttcc gtttccggcg gattcacgcg cggttcgaaa tcagaaacgc cggcccatgt
      661 ggctacggct ttcacgcgtt tgtcctgcgc ggctgcgagc agcgcaattc caccgccccg
      721 gctgtggccg agcaaaccga gccgcgacgg gtcgaaaaca gaatcatgtc cgacatgcca
      781 cgaaaggctg tcgatcacca gcgagacatc atcgagctct tttgaaaatg tattttttcc
      841 gaacaggtcg gggcgaacaa attccacctg gttctccggc gaggtcccgt tgtgcgaaaa
      901 attaaaacgc actaccgcaa aacccgcgcg cgaaagatgt tctgccacca gcggccagtg
      961 tccccagtcc ttgaaacctt tgaacccgtg cacgaaaacg atcacgggca gcggcgattt
     1021 tcccaatggc ggaagatgga aatccacggc cacgggttca cggttctcca ttcgcaggac
     1081 gtcgcggatg atcctggtca ttctggtttt ctgaaagcgg aaggtgaaac gtagtactcg
     1141 atccggcaaa gacggtcgtc ggtgaatttg tacgtcgcgg tgtaatcctt ccaggaataa
     1201 tagtgataat atttcgcgtc cttctgctga ggatgatatt gcaggtaaag cgtgtcgcct
     1261 gtcatccatt ccatcatcgg ctgatctgtg taaaccgact gcacaaaata agacgacatg
     1321 ccgaggtaaa tgccgtagcc ggtcatgaaa tgcctgtccc tcgcgtaaac tgtgtcgccc
     1381 tgcggcatga tcacgctccc ggaaccgggc cgctgaagag cgagcatata aggtgtttca
     1441 ttcccgtttt cgtcatcata cacataaacg tccagctgct cattccccga agggttcagc
     1501 catgaaacac cgtatgtatt ctccccgatc ttttcacccg gcgggccctg cgcatgatgc
     1561 ctgacaatat gcccggcaga agaataaata ttattgtcgc ttcgctggct gcgctctccg
     1621 catttctgcc accatttttc aaaggaggac cggcttccga ggacaagatc gtttacgcag
     1681 gaatccggat cgaagcccca ggaggaaact tttcctttgc gcacttcttt cggaacaggc
     1741 ccgggcatgt cttttttcgc agtatccgca gcctgcgcac tgcctgtatc gtccgggctg
     1801 atcggtatcc cgtcgagaca ggacgtgaaa acaaaggctg cgaagaaaaa cggaaaatat
     1861 tttttcatgg agatgccggg gcttgcgcgc atttcccaaa gttattttgc gcagcagaat
     1921 tcccggcata ccagcccgga aatatttcat gaaccggcgg ctgttgattt tctttcccgg
     1981 ccgtgaaaga ttcttcctgt acctttgcac aatcgcttcc gctgcgtggc acacccatcc
     2041 caaaaacaag ttcacgatcc ttacgcttcc ctgcgtatcc gggacttcag catttacctg
     2101 gctgcgcgtt tcttcatgac gcttggcgtg cagatccagg gtgtcgtggt cgggatacag
     2161 gtgtacaaac taacgcacag cgcattccag ctcgggctca tcgggcttgc cgaagcgatc
     2221 cctttcatcg ggctttcttt tttttccggg tacgtggcgg acatcatccg caggaaaaag
     2281 atcattctcg cttccacgac tttcctgctg ctggcgtcat ggtcgctctt ctttgtctcg
     2341 cataatgtcg cgctgctgca cgaactcggc gcatggccca ttttcggtat tattttctgc
     2401 acgggcatcg cgcggggatt tctcggacct gtgtttcccg cttttctttc ccagctcgtt
     2461 ccgcgaaaac tttatcccaa cgcaacgacg tggcagagca acctgtggca gactgcggcg
     2521 gtgctgggtc ccgcaatggg cggaatgctc atcggctggg agagcgtaat tgtcgcctac
     2581 ggcacctgct cttcgctcat cacgctttcg tttttcctga tgatgcttgt tacgaatcac
     2641 ccgcttccgc cgatcgaaaa aaaggaatcg ctggtgaaaa gcctgaccgc aggcttccgt
     2701 tttgttttca gcaaccaggt gatgctggcc gcattttcgc tggatctttt cgcggtgctt
     2761 ttcggcggcg cggtggcgat gatcccggtc tttgccaacg aagtgctgca cgcaggaaaa
     2821 gatgctgatt ccattatcgg ctggctgcgc gctgcgccgg ccatcggtgc gatcgtttcg
     2881 gggtttctgc tggcccgttt tccgccgacg aaacatgcgg gaagaaacct gttcatcgcc
     2941 gtgatcggtt tcggctgctg catcatcggg tttgcgctct cggacatctt cctgctctcg
     3001 ttcatttttc ttctgatcag cggcgcgttc gaccaggtca gtgttgtcgt gcgtcaatcg
     3061 atacttcagc tgcttgcacc ggacaacatg cgcggaagaa tagccgcagt gaacggaatt
     3121 ttcatcggtt cctccaatga gatcggggaa tttgaatccg gtgtgacagc caaaatgatg
     3181 ggaaatattc cttccgttat tttcggcggc tgcatgacga tcgccatcgt cattgccgtg
     3241 atgttctcgg cgccgaagct gagaaatctt gatcttgcga agatcacctg aaaaaaagcg
     3301 cgccgggagc tccggcgcga ccagggaaaa cttcacctgt tttcccgcaa cattgccggc
     3361 agggttcacc ggctgctgcc catgtttttg ttatttcatt gacgccgaag ttatgtgctg
     3421 ccgtacgcgg ccggaaacgg atttccgcca ggattttgcc ggaaacgcgg aatattcaaa
     3481 atcatgggtt tatgttaggt aatgcggttg ttttttgcga ttttcgcctt tgacctgcac
     3541 gcatttgcgt tttttactac tctttttcaa acatgaagtc ccagactaac cccaaaacgg
     3601 gtgagccgcg cacagtccag gctgagttca tcagccggct gaaaacggtg atcccgccca
     3661 atcactccct ggttgatgag cttgcagaca tcctgcaggt gagcaccgac agcgcctacc
     3721 ggcgcatacg cggggaaacc gcgcttacga tcgacgaggt cagccgcctc tgcagccatt
     3781 accgcattcc gttcgatcac ggtgcagtga agaacgggaa ggcgagcgtc acgttcagct
     3841 atcaccatct ttcggacaac tcgtccaatt ttatcgatta cctcaacagc gtcgtttccg
     3901 atctcaagcg gatcaaggcg acggaaccga acgagatcat ttttgccgca gaagacatcc
     3961 cgatctttca ccatttccgc ttcccgctgc tgacggcgtt caagcttttc tactggagca
     4021 aatccattct caatgtgccc gaaatggaag agctgcgctt cgatccggct tccgttccgg
     4081 aaaaaccggt gaagcttgtg cgcgagatct actcgctgta ccagaccatt ccttccatcg
     4141 agatatggac cgaagaaacg ctcgactcca cgctcaagca ggtggaatat tattacgaat
     4201 cgggactttt ccagtcgaag gaacaggcca tcgaactgct ggccgagatc ggtaaaatga
     4261 tcgccggcgt ggaaaagcag gcggcgctca gcatgaaatt caccggcgac gtaccggatg
     4321 cgaattcaaa cgtgaattac tcgctgtaca attccgacct gatgatgggc aataactgca
     4381 ttctcgtcac ggccggcggc gcgcgtttca cgtatctcgt gcacaacacg ttcaactcca
     4441 tgctgacgac gagccagagc ttcaatgaag agaccgaacg ctggctgcgc aatctcatcc
     4501 gtaaatcgaa cccattgagc ggcgtttccg aaaagcagcg cttccgtttt ttcaaaaaac
     4561 tgaatgacaa gatcgcgcgc acacgcgaag gcatcagcgc cgggtaaact ggcgccgggt
     4621 gagctgcggg ccttcctcga attgaagacc ggggaataca accgtccgtc gttcatcgcc
     4681 agcgacccgg tttccattcc gcatttattt tcccgcaaag aagacatcga aatttccgcg
     4741 ttcattacgg ccaccattgc atgggggcaa cgccccgtga tcatccgcaa cgcgaaacgg
     4801 ttcatggaac tgatggagat ggcgccgtat gaatttgttc taaccgcttc ggaaaaagaa
     4861 cgcgcccgtc tcgggaaatt cgttcaccgt accttcaacg gcattgacgc ggtttatttt
     4921 cccggagcgc tgcgcaatat ttacgcgaag cacggcggaa tggaaaatgt tttttcagag
     4981 ggattgctgc gcgcggagaa tgatttcgcc atgagcgcag cgatcgccca tttccgcaac
     5041 gtcttctttt cgctgcatca tcagaagcga acggaaaagc atgtgtccga tccggtttcc
     5101 ggttcgaatg caaaacgcat caacatgttc cttcgctgga tggtgcgcaa agacaacgcc
     5161 ggtgtcgatt tcgggatatg gaaaagtatt tcaccttcgc tcctgctctg cccgctcgat
     5221 gtgcacgtgg gcaacacggc gcgcgcgctg ggacttttga aacggaaaca ggatgatcgc
     5281 aaagccgtgg aagaactgac aggaagtctt cgcctgctcg atccgcacga tcctgtgaaa
     5341 tacgattttg cgctgttcgg cctcggtgtg tttgaaaaat tcggcacgcg gtgatgagct
     5401 ttttcatcgc gttttttctg catgcagagt gaatccttca ttgcgccgga aaaatagtgc
     5461 gcgcgggtcc cggatctgct cagggattcg aaaaaaaatg atcgaagcga aaccccgtcc
     5521 ggacggcgga tataccgccg gtaaaaagcg gattccatct ccctgcattt tccataactt
     5581 caacccttct tt
//
LOCUS       DASTSF010000620        15081 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_942940,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000620 DASTSF010000000
VERSION     DASTSF010000620.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 15081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15081
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_942940"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(236..643)
                     /locus_tag="VFU15_17420"
     CDS             complement(236..643)
                     /locus_tag="VFU15_17420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019975860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HEU4719630.1"
                     /translation="MKKPVSKAVTDNRTRYSDKELAEFKTLIIGKLNEASRDYELLKN
                     TLSHKDDHGTDDTSPTFKLLEDGSDVLSKEETAHLAARQEKYIQNLKNALIRIENKTY
                     GICRVTGKLIPKERLRSVPHATLSIDAKLEQKN"
     gene            complement(955..1083)
                     /locus_tag="VFU15_17425"
     CDS             complement(955..1083)
                     /locus_tag="VFU15_17425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719631.1"
                     /translation="MNEPMVHDEEPAMDMNDDVTEMNDEGDDNSEGNDMRGAFDHF"
     gene            1070..1465
                     /locus_tag="VFU15_17430"
     CDS             1070..1465
                     /locus_tag="VFU15_17430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719632.1"
                     /translation="MGSFMTGGGVFSTTGFGAAGFLGATGFFGAAGFFGAAAFAGAAF
                     LATAFFFGAAFFLGAAFLAAAFFFGAAFFLGAAFFFGAAFLAGFFLAAFFGAFLAAFF
                     TAFLAAFFLAGFAFLAPPFFAFFAMGVEI"
     gene            complement(1462..4947)
                     /gene="ileS"
                     /locus_tag="VFU15_17435"
     CDS             complement(1462..4947)
                     /gene="ileS"
                     /locus_tag="VFU15_17435"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEU4719633.1"
                     /translation="MKKYPEFNSLNLPAIAEKVLADWDQNNVFEKSVTTREGAEPFVF
                     YEGPPSANGKPGIHHVMARTMKDLFCRYQTLKGKQVKRKAGWDTHGLPIELSVEKTLG
                     ITKDHIGKPKEEGGISIEDYNKACRTEVMKYTHLWEELTRQMGYWVDMSDPYITYENS
                     YIESVWWLLQQLYKKNLLYKGYTIQPYSPAAGTGLSTHELNQPGCYRPVKDRTATAMF
                     KALTTGLGKKSADDVYFLAWTTTPWTLPSNTALAVGVKINYAVVKTFNPFSHQLVHVI
                     LAKDLVGKYFPEKNAELKFEDYKPGDKAIPFKIVNEIPGADLVGSKYEQLLPYAKPYD
                     DPNGEKSFRVIAGDFVSTEDGTGIVHIAPTFGADDMRAAKAAGVPPMLVMDENEKPVP
                     LVDLHGRFRKEVNDPQFGLAGEYVKEDYLTEREKAEELAKQKEYMKFLIKDTSKLNYL
                     SVDERIVLKLQNEGKLFKKETYEHNYPHCWRTDKPVLYYPLDSWFIKASSKKEEMSKL
                     NKTINWKPESTGSGRFGEWLNNLQDWNLSRSRFWGIPLPIWKSEDHSEVVCIGSAEEL
                     KAEIERSMKAGFMKKNPLEKFVPGNFSKENYSSFDLHRPYADEIILERDGKKLFREPD
                     LIDVWFDSGSMPYAQLHYPFENKEQIDNRTYFPADFIAEGVDQTRGWFYTLHAIATML
                     FDSVAYKNVISNGLVLDKNGNKMSKRLGNAVDPFETLKKYGPDATRWYMVTNASPWDN
                     LKFDPEGILEVQRGFFGTLYNTYNFFAMYANIDGFVYDEKNSVPVAERSELDRWIISR
                     RNSLVKFVTLRLDDYDPTPAARAIQNFVDEHLSNWYVRLSRRRFWSVRQSAESPENAK
                     DKQSAYETLYECLRTVAQLSSPFAPFFSDWLYGNLGEGTSVHLSHLEKANGALIDSEL
                     EERMDLAQRFSSLVLSLRKKVNIRVRQPLNRIMVPVLDDTFRARMELVKSIILAETNV
                     KNLEYVTESGIFVRKIKPDFKALGKKLGKHMKAAAAAFAEFSQEDILKMETQGYFALS
                     LDGEVIRIERSEVEILVDDLPGWLVAADGRLTVALDVTITQELKEEGLARELVNRIQN
                     LRKDKDFEVTDRIEVKIKSHELLASAVRNNFNYICSETLATSLELVNDLGEREAVPVE
                     VDEEIKTLVHISKHN"
     gene            complement(5152..5805)
                     /locus_tag="VFU15_17440"
     CDS             complement(5152..5805)
                     /locus_tag="VFU15_17440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719634.1"
                     /translation="MKNFRVILFFSCALIAVSCGKRRKVIGGGTLYFPQTGKETWLLY
                     LENDSCYEAFYSGNTLVRIISAKTKEEMEDSTYLERCYFPDGKLLLTKQFIHRVPEGE
                     WTTYYDTGKQKSWKVIRHGVPLHYKDWFEDGRLSVEGIRDPNGNLDRKQYYYNGNMDQ
                     EFTTDSLGNGHCKAYHKNGKIETEGQLNYFSPFGLWKRWDSLGNPRTDTVYGIPGNR"
     gene            complement(5802..6218)
                     /locus_tag="VFU15_17445"
     CDS             complement(5802..6218)
                     /locus_tag="VFU15_17445"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002695971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEU4719635.1"
                     /translation="MSESINSSKAPEPVGLYPHAKRVGNLLFLSGVGPREKGTKKIPG
                     VELDENGKIVSYDIEKQCRSVFNNVKNILEDSGSSWNNIVDVTVFLTNMKDDFPVYNK
                     LWAEYFSENPPCRTTIEINCLPTPIAIELKVIATAG"
     gene            complement(6419..7831)
                     /locus_tag="VFU15_17450"
     CDS             complement(6419..7831)
                     /locus_tag="VFU15_17450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719636.1"
                     /translation="MNDSTNAFRIVVISVAAALVVPALFMDGMFADGMLYSTVAKNLA
                     AGHGTWWDPHFTVFMHDHFHEQPPLVIWLQSFFFLFAPHSIYPERFYDLVLMAVNTWL
                     IMRFWRTIFYGNADWQKLDWLPVLLFFIMPVTFWGFDNNIIEITMSAFVLASATAFFR
                     AFVHEKKVRLNVFLGITWLIAATLCKGIQGGFTLVLPLLLWLAGIKLSFGKAFRATLV
                     ITGGFVLFYGIIFLFPAVRHAYVVWFNQRIVTTFSGVNNTSGSRFHMLFELLLDVLPP
                     AGFLAFATLLSRQWPVKNLAAEERKNLVFILLLALAGSLPLMVTKEQRGFYLVTALPF
                     YAVFFAILCAPAFVMRVKKIGAAAGKLILSAGILILCATIVATFIFAGKPKRDAEKIS
                     DITAIGQVTGGNTILGCDRAMVTDYAVVSYCARLYNISITDDTTMSGISYKISFSSQT
                     PTGFSPVNVKTKEIFLFRKN"
     gene            complement(7842..8711)
                     /locus_tag="VFU15_17455"
     CDS             complement(7842..8711)
                     /locus_tag="VFU15_17455"
                     /inference="COORDINATES: protein motif:HMM:NF015818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HEU4719637.1"
                     /translation="MGQTEKIKGYFRRLAENGYGHVSLQRVSPVTGGSINSCVRLETD
                     KGWFFLKENDAQKFPGMFAAEAKGLQLLAGTKTFVVPHVIAQWEERDSSFLLMEWMEH
                     SRSGNSYEAGTQLAKLHRHTEKFFGLGHDNYIGSLPQSNKKHSSWEDFFSLERILPQV
                     KLARDSGKTDKTISAQAENFCARIAEIFPPGQPSLLHGDLWSGNFFFTGKGPAVFDPA
                     VYYGHREMDLAMTKLFGGFDADFYAGYANEFPPEKNHAQRADYCNLYPLLVHVNLFGG
                     GYLYDVKSILKNF"
     gene            complement(8690..9256)
                     /locus_tag="VFU15_17460"
     CDS             complement(8690..9256)
                     /locus_tag="VFU15_17460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010571588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEU4719638.1"
                     /translation="MANVKPRSYCEAVSKMEKGNVHVHYHDKEYGFPIDDDNQLFARL
                     VLEINQAGLSWTTILKKKENFFRAFDNFEISRVAKYKSREIARLLSNEGIIRNRLKIE
                     ATIHNANAILALQKEFGSFRQWIDHHHPKTKEEWVKIFKKTFRFTGGEIVNEFLMSTG
                     YLPGAHIPSCPVYKKVMKTKPAWARPKK"
     gene            complement(9318..9794)
                     /locus_tag="VFU15_17465"
     CDS             complement(9318..9794)
                     /locus_tag="VFU15_17465"
                     /inference="COORDINATES: protein motif:HMM:TIGR03511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility lipoprotein GldH"
                     /protein_id="HEU4719639.1"
                     /translation="MRYSVLLPFAFAAMLLFASCDRTRFFEENRDIPNNTWDMNNRLS
                     FDFDVPDTITKYNFCFNIRNNEDYPYRNIYVFFHTTFPNGKMANDTVDFPLIDESGRW
                     AGKGQGDVHDCRLVFRQNVRFPLAGKYHMEVEQAMRMEKLPGVLNAGIRIERAQPR"
     gene            complement(9797..11164)
                     /gene="ricT"
                     /locus_tag="VFU15_17470"
     CDS             complement(9797..11164)
                     /gene="ricT"
                     /locus_tag="VFU15_17470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HEU4719640.1"
                     /translation="MGCSGCSSGRGCGTVVNGVPAGCRSNGSCGIGGCNKLNVFDWLA
                     NMELPSGQAPFDVVEVRFKNSRKEFFRNVNNLPLVTGEVIAVEGSPGHDIGTVSLTGE
                     LVKTQMARRGVKIDDQAIRKVYRKAKENDVEKWKEAQALEQSTMMKSREIAQRLGLQM
                     KISDVEFQGDKTKAIFYYTAEERVDFRELIKILAEQFRIRIEMRQIGARQEAARLGGI
                     GSCGRELCCSTWLSDFRSVTTSAARYQQLSLNPLKLAGQCGKLKCCLNYELDSYLDAI
                     RDFPETADIETSRGRARHQKTDIFRGIMWYSFADEPDQFIPLSTDRVKEIIKLNKEGV
                     KPEDIGAVKDKKEAVEKAPDFENVVGQDSLTRFDKSKKKKRRKPSGQRNTGGQPQNTQ
                     QKPQQKNPQQKQPRPQQQKNQPRPNNPQQQNQQKNNPQRNQRRNRPPRPGGGNKPGTP
                     PAPKN"
     gene            complement(11263..12399)
                     /locus_tag="VFU15_17475"
     CDS             complement(11263..12399)
                     /locus_tag="VFU15_17475"
                     /inference="COORDINATES: protein motif:HMM:NF024575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEU4719641.1"
                     /translation="MLFQEIIGQRDTRERLVNSVRSQRISHAQLFFGHPGSGALPLAL
                     AYAQYVHCTNRKENDSCGECPSCVKYARLVHPDLHFVFPIALSKDVRVSTHVIAEWRE
                     AFLENPYLTLDEWFTKLEAENKQAIIGTEESGEILRKLSLTTFEGEYKIMIVWLAEKM
                     NAASANKLLKILEEPPDKTLFLVVTENEDQLLRTIVSRTQLVRIHAIADADLRDALVQ
                     RHSLSPADAEKIAFQAEGNYAEARSLISRSEQAAFNLSTFTQWMRASLKFDVAKVLSA
                     VDDLAGIGRERQKQFLSYALGLVRECLLINYADPSLVRMTGEELAFIRKFSPFIHAAN
                     GEAFMNELSKAIQHIERNGSPKIIFTDLSFKANELLNIPKPQAV"
     gene            12676..>15081
                     /locus_tag="VFU15_17480"
     CDS             12676..>15081
                     /locus_tag="VFU15_17480"
                     /inference="COORDINATES: protein motif:HMM:NF024962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719642.1"
                     /translation="MNVNGGIPVHTNISGGQDQVDLCVTNVHDGIIFVWRDSRSGSNG
                     QDIYAQKLTPCGDVAPGWPASGLLVCNAAGNQQVPQIVPDTSGGAVITWQDTRGADWD
                     IYAQHVSSAGVCTWTANGVAVCTATGNQQNPHLCIDGTGGAVICWDDMRSGNWDVYAA
                     KVTGSGTTPWTANGVPLVSLSGTQNNNCIATDSAGGAFFAWEDSRPASNGIDLYAIRL
                     NTNGNVPGGWPANGLALSTQTSNQKEPCACNTGGQTAMIAWADGFGAGQSILASRLNG
                     SGGYVAGWSYPGNLVGNAITQSGNPGTAELPKIAPDCGGGAYISFGSHYSSSDHDVLT
                     QHMGFNGIPSGGLETTAFNTDIEHYQEICADGNGGAVNVWQDQWPPAIVWYRKFGSGG
                     VIASQFVSSTYPDVAAMRGVWYAAWENNGGAAVIGSSDIYAISSSASAPPPILNSTLT
                     QTYTGTSCDITTQNVSLCAGSPITFSVPLPRGLYNTYTFLDGATVLQTGTSASYTTSA
                     LTVGTHTITATSSISGSCVTAASNQIVVTVTAGPAVTLSSQTDVLCNGGSTGSATMNA
                     TGGSPAYTYAWTPAGGNAATASSLAAGTYTCLVTDQLGCAQTQTVTITEPAAITATVS
                     STAATCGNTNGSATVNPAGGNGSYSYVWSPSGGNASTASSLAAGSYTCAVTDGNGCSQ
                     NFPVSVNSTGGPTVTPASQADVSCFGGANGTASVTVTGGTPGYSYAWTPSGGNAANAS
                     GLGAGTYTCTVTDGLGCVQSQTFSITQPTALSATSSVTNVACNGGLTGSATVNPSGGA
                     GGYS"
BASE COUNT         3115 a         3989 c         4181 g         3796 t
ORIGIN      
        1 tagaaccagc ttcccgtcac tttgtacgct tccccgagat acatgtgcgt tttgatccag
       61 atcttgatcg actggtcgat cagcagaacg gaaaaaatga tggcgagcgc ttttttcaaa
      121 cgggaattta tttcggagca cgaatatacg aagagcgcgg gcgttaaaag cagaaaagcc
      181 ttcccgtttt cggtgaaggc ttttcgttaa caaaaattat atgcagaaga gcagatcagt
      241 ttttctgctc aagctttgcg tcgatgctga gcgtcgcatg cggcacgctg cgcaggcgtt
      301 ctttcgggat gagcttcccg gtaacgcggc agatgccgta cgttttgttc tcgatgcgga
      361 tgagcgcgtt cttcaggttc tggatgtatt tttcctggcg cgcggcaagg tgtgcggttt
      421 cctctttgga aagcacgtcg gagccgtctt cgagcagctt gaacgtcggt gacgtgtcat
      481 ctgtcccgtg atcatccttg tgcgagagcg tatttttcag cagctcataa tcgcgtgaag
      541 cttcgttgag cttgccgatg ataagtgtct tgaactccgc gagctctttg tcgctgtaac
      601 gcgtgcggtt gtccgtcacg gctttggaga caggcttttt catggccggg gcaggtttct
      661 gcatcacggc aacaggcttg acaaccgggg gcgttttggg agcgggagga gctggtttgg
      721 ctttcgcaac gggttttgcc gtcttcttgg cgggagctgc ttttacttta gcaaccggtt
      781 tcgcttttga cggtttttta gccggggcgg ccttggaaga tttggaaggt ttcttcgaag
      841 acttcgctgc tttggaagca gccccggatt ttttcgccgg agctgctttt tttgcaggct
      901 ttgaaggctt cttagcgttt gcttttttag ccattgtaaa gaataaaatt cagattaaaa
      961 atgatcgaag gcgccgcgca tgtcattgcc ttcgctgttg tcatctcctt cgtcgttcat
     1021 ctccgtaaca tcgtcgttca tgtccatggc aggttcttca tcgtgcacca tgggttcgtt
     1081 catgaccggc ggcggtgttt tttcaacgac gggtttcggt gcggcaggct ttttaggcgc
     1141 aacgggtttt ttcggtgcag caggtttctt cggagccgct gcttttgcgg gagccgcttt
     1201 tttagctact gcttttttct tcggagctgc tttctttttg ggagccgctt ttttggccgc
     1261 tgcttttttc ttcggggccg ctttcttttt gggagctgct tttttcttcg gagccgcttt
     1321 cttggcaggc ttctttttag ccgccttttt cggtgctttt ttggcggctt tctttaccgc
     1381 ttttttggcg gcttttttct tggccggttt cgctttttta gctccgcctt ttttcgcctt
     1441 ttttgccatg ggtgtggaga tttaattgtg ttttgagatg tgtactaacg tttttatctc
     1501 ttcatccact tcaaccggaa cggcttcccg ttcaccgaga tcattcacga gttcaagcga
     1561 agtcgcaaga gtttcagagc aaatataatt gaaattattt ctcacagccg acgccagcaa
     1621 ctcgtgcgat tttatcttca cttcgatcct gtccgttact tcgaagtcct tgtccttccg
     1681 taagttctgt atgcgattga ccagctcccg ggccaggcct tcttctttca gctcctgcgt
     1741 gatcgtgacg tccagcgcta ccgtcagacg accatccgcg gccaccagcc agcccggcag
     1801 gtcatccaca agtatctcaa cctcagaccg ttctatgcgt atcacttccc cgtcaaggga
     1861 taatgcgaag tagccctgcg tttccatttt caggatgtct tcctgcgaaa actccgcaaa
     1921 agcggccgcc gcggctttca tgtgcttccc gagctttttg cccagcgcct tgaaatcggg
     1981 cttgatcttg cgtacaaaaa tgcccgattc ggtcacgtat tcaaggtttt tcacgttggt
     2041 ttcggccagg atgatcgatt tcaccagctc catccgtgcc cggaacgtat catccagcac
     2101 cggcaccatg atgcggttca gcggctggcg cacgcggata ttgacctttt tgcgcaggga
     2161 aagcaccagc gaggagaaac gctgcgccag gtccatacgc tcttccagct cggaatcgat
     2221 gagcgcgccg ttcgcttttt cgaggtgcga aaggtgaaca gacgttcctt cgccgagatt
     2281 gccgtacagc cagtcggaaa agaaaggcgc gaacggggag gaaagctgcg ccaccgtgcg
     2341 caggcattcg taaagcgttt cgtatgcgga ctgcttgtct ttcgcgtttt ccggcgattc
     2401 ggccgactgg cgcaccgacc agaaacggcg gcgggaaaga cgcacgtacc agttcgacaa
     2461 atgctcgtcg acgaaattct gtatggcgcg tgcggcgggc gtgggatcgt aatcgtcgag
     2521 gcgaagggtg acgaatttta cgaggctgtt cctgcgcgag atgatccagc ggtcgagctc
     2581 ggaacgttcg gcgacgggaa cggaattctt ttcgtcgtag acgaacccgt cgatgttggc
     2641 gtacatcgcg aagaagttgt acgtgttgta cagcgtcccg aaaaatccgc gctgcacttc
     2701 gagaatgcct tcggggtcga atttcaggtt gtcccacggc gaagcgttcg tcaccatgta
     2761 ccagcgcgtg gcgtcgggac cgtatttttt cagcgtttcg aacgggtcga cggcattgcc
     2821 gaggcgcttc gacattttgt tgccgttctt gtcgagcacg agcccgttgg agataacgtt
     2881 tttgtacgcc acggaatcga acagcatcgt ggcgatcgcg tgcagcgtgt agaaccagcc
     2941 gcgtgtctgg tcgacgcctt ccgcgatgaa atcggcagga aagtaagtgc ggttatcaat
     3001 ctgttctttg ttctcgaacg ggtaatgcaa ttgcgcatac ggcatggatc ccgaatcaaa
     3061 ccacacgtcg atgagatcgg gttcgcggaa taatttttta ccgtcgcgct cgagaatgat
     3121 ctcatccgca tacgggcggt gaagatcgaa cgagctgtaa ttttccttcg agaaatttcc
     3181 cggaacgaat ttttccagcg ggttcttttt catgaatccc gctttcatgg aacgttcaat
     3241 ttccgctttc agttcttctg ccgagccgat gcagacaact tccgaatgat cttccgattt
     3301 ccagatcggc agcggaattc cccagaaacg cgagcgcgac aaattccagt cctgcagatt
     3361 attcagccat tctccgaaac gcccgcttcc cgtgctttcc ggtttccagt tgatcgtttt
     3421 gttcagcttc gacatctctt cctttttcga agaagctttg atgaaccagg aatcgagcgg
     3481 gtaatacaaa acgggcttgt cggttctcca gcaatgcggg taattgtgct cgtacgtttc
     3541 ttttttgaag agctttcctt cgttctgcag cttgagcacg atacgttcgt cgacgctgag
     3601 gtaattcagc ttcgaggtgt ctttgatcag gaatttcatg tattccttct gcttcgcgag
     3661 ctcttccgcc ttttctcttt ccgtgaggta atcttccttc acgtattcgc cggccaggcc
     3721 gaactgcgga tcgttcactt ctttccggaa tcttccgtga agatcgacaa gcggaaccgg
     3781 tttttcattc tcatccatca cgagcatcgg gggaacgccg gccgctttcg ctgcgcgcat
     3841 gtcgtctgca ccgaacgtag gcgcgatgtg gacgattccc gttccgtctt ccgtcgacac
     3901 aaaatcaccg gcgatgacgc ggaaggattt ttctccgttg ggatcgtcgt aaggtttggc
     3961 gtaggggagg agctgttcgt atttagaacc gacgagatcg gcgccgggaa tttcatttac
     4021 aattttaaac gggatcgctt tgtcgccggg cttgtaatct tcgaatttca gctccgcatt
     4081 tttttccggg aaatatttac caacgagatc ttttgcaaga attacatgca caagctgatg
     4141 agaaaacggg ttgaatgttt ttacgacggc ataattaatt tttacaccca cagccaatgc
     4201 cgtatttgaa ggcagcgtcc acggagtagt cgtccaggca agaaaataaa catcatccgc
     4261 actttttttt cctaaccctg ttgtcagagc tttgaacatc gccgttgccg tccggtcttt
     4321 cacggggcga tagcaacccg gttgattcaa ctcgtgggtg gacaatcccg ttcctgcggc
     4381 gggcgaatag ggctggatgg tgtagccttt gtacagcaga tttttcttgt acagttgctg
     4441 cagtagccac cacacgcttt cgatgtagga attctcgtac gtgatgtacg ggtcgctcat
     4501 gtcgacccag tagcccatct gccgtgtaag ctcttcccag aggtgcgtgt atttcatcac
     4561 ctcggtgcgg caggctttgt tgtaatcctc gatggaaatg ccgccttctt ctttcggttt
     4621 gccgatgtga tctttcgtga tgccgagcgt tttttccacg ctgagctcga tgggaagccc
     4681 gtgcgtatcc cagccggctt tgcgtttcac ctgttttcct ttcagcgtct ggtaacggca
     4741 gaaaaggtct ttcatcgtgc gcgccatcac gtggtgaatg ccgggttttc cattggcgga
     4801 cggaggtcct tcgtaaaaaa cgaaaggttc ggcgccctcg cgcgtggtga cgctcttttc
     4861 gaaaacgttg ttctggtccc agtccgcgag cactttttcg gcgatcgcgg gaaggttaag
     4921 cgaattgaat tccggatatt ttttcattgt ccgcaggact gcatttgagg agggcaaaga
     4981 taggaaaagt gcccctgccc ggtctcgact cacgctcgac ctgacggtgg tttcgacttt
     5041 cgcccgacca gacagtggtc tcgactgggg cccgacccga tgggaggaaa attttcctcc
     5101 gcaggtcagg ctgagtagtc cgcgaggacg tatcgaagcc tgacctgcgc gttaccggtt
     5161 acccggaatc ccataaaccg tatctgttcg cggattcccg agagaatccc atcgtttcca
     5221 gaggccgaac ggggaaaaat agttcagctg gccttcggtt tcgatcttcc cgttcttgtg
     5281 ataggctttg caatgcccgt tgccgagaga atccgtggtg aattcctggt ccatgttgcc
     5341 gttatagtaa tactgtttcc ggtcgaggtt tccattggga tcgcgtatgc cttcgaccga
     5401 gagacggccg tcttcgaacc agtctttgta atgcagcgga acgccgtgcc ggatgacctt
     5461 ccatgacttt tgcttcccgg tatcgtaata agtggtccat tcgccttcgg gaacgcggtg
     5521 gatgaactgt ttcgtcagca ggagcttgcc gtcggggaaa tagcagcgtt cgagatacgt
     5581 gctgtcttcc atctcttctt ttgtttttgc gctgatgatg cgcacgagcg tgttaccgct
     5641 gtagaaggct tcatagcagg aatcgttctc gaggtaaagc agccaggttt cttttcccgt
     5701 ttgcggaaaa tacaatgtgc cgccgccgat gaccttcctc cgttttccgc aggaaacagc
     5761 gatcagcgcg caggaaaaaa acaggatgac gcgaaaattt ttcatccggc agtggcaatg
     5821 actttcagct cgatggcgat gggcgtggga aggcagttga tctcgatcgt tgtgcggcag
     5881 ggcgggtttt cactgaagta ttccgcccac aatttgttgt aaacgggaaa atcatccttc
     5941 atgttggtga gaaaaacggt gacgtcgacg atgttgttcc agctgctgcc ggaatcttcg
     6001 agaatgttct tcacgttatt gaaaacgctg cggcattgtt tttcaatatc gtaggaaacg
     6061 attttcccgt tctcatccag ttccacgccg ggaatttttt tcgtgccttt ttcgcgcgga
     6121 cctacgccgg aaagaaagag aagatttccc acgcgttttg catgcgggta aaggcctacg
     6181 ggttcgggcg ctttcgaaga gttgatggat tctgacatct tgatcttgat ttcggattcc
     6241 gaagttcggg tttttccggc aaccgttgcc cggggaattc ctttgccgtg aaacggatcc
     6301 tgtgattctc tttgatcaaa agctcacgca acggcgggaa gggacaaaga aatgctgccg
     6361 gcgttctgct tcgtgcaaag cgaagtcctt tattcgctgg tttcgaaaaa tgaaagcatc
     6421 agtttttccg gaacaggaat atctctttcg tcttcacatt cacaggggaa aaccccgtcg
     6481 gcgtttggga ggaaaacgaa attttatacg agatgcccga catcgtcgtg tcgtccgtaa
     6541 tgctgatgtt gtaaagacgt gcgcaatagg aaacgaccgc ataatccgtg accatcgcgc
     6601 ggtcgcagcc gaggatggta ttgccgcccg tgacctgccc gatggccgtg atgtcggaaa
     6661 ttttttccgc gtcgcgcttc ggctttccgg cgaaaatgaa tgtggcgaca atcgttgcgc
     6721 agagaatgag aattccggcg gacaagataa gtttcccggc cgctgccccg atcttcttta
     6781 cgcgcatcac gaacgcaggc gcgcaaagta tcgcgaagaa aacagcatag aacggaagcg
     6841 cggtgacgag gtaaaacccg cgctgctctt tcgtcaccat cagcgggagc gacccggcaa
     6901 gtgcgagcag gagaatgaaa acgagatttt tcctttcctc tgccgcgaga tttttcacag
     6961 gccattgacg cgaaagcaat gttgcgaacg cgaggaagcc ggcgggagga agcacgtcga
     7021 gcagcaattc gaacagcatg tgaaaacggg aaccggacgt gttgttgacc cccgaaaatg
     7081 tcgtaacgat acgctggttg aaccacacga cgtatgcatg gcgcacagcg ggaaaaagaa
     7141 aaataatccc gtagaagagg acgaatccgc cggtgatcac cagcgttgcg cggaacgctt
     7201 ttccgaacga aagctttatc ccggcaagcc agagcagcag cgggagcacc aaagtgaagc
     7261 cgccctgtat tcctttgcac agcgtcgctg cgatcagcca cgtgatgccg aggaacacgt
     7321 taagccgcac ttttttttca tgcacgaacg cccggaagaa cgccgtggcg gatgcgagca
     7381 cgaaggcgct catcgtgatc tcgatgatgt tgttgtcgaa tccccagaac gtcacgggca
     7441 tgatgaagaa cagcagcacg ggcagccagt cgagcttctg ccagtccgcg ttcccgtaaa
     7501 agatcgtccg ccagaaacgc atgatgagcc aggtgttcac ggccatcagc acgaggtcgt
     7561 aaaaacgttc gggataaatg gaatgcgggg cgaacaggaa aaagaaagat tgcagccaga
     7621 tcaccagcgg cggttgttcg tggaaatgat cgtgcatgaa aaccgtgaag tgcggatccc
     7681 accaggttcc gtgacctgcc gcgagatttt tcgctacggt ggaatacagc atgccgtcgg
     7741 cgaacatgcc gtccatgaac agcgcgggca caacgagcgc cgcggcgacc gagatgacga
     7801 cgatgcggaa tgcgttggtg gagtcgttca cgggaagcgc tttagaaatt tttaaggatg
     7861 cttttcacgt cgtaaagata accgcctccg aacaggttca catgcacgag cagcgggtag
     7921 aggttgcaat aatccgctcg ctgtgcgtga tttttttcgg gcgggaattc attcgcgtag
     7981 ccggcgtaga aatcagcgtc gaatccgccg aataatttcg tcatggcgag atccatttcc
     8041 cggtgaccgt aataaacggc cggatcgaaa acggcgggac ctttcccggt gaagaaaaaa
     8101 ttcccgctcc agagatcgcc gtgcagaagc gacggttgtc cgggcggaaa aatctcggcg
     8161 atgcgcgcgc agaaattttc tgcctgcgcg gaaatggttt tgtctgtttt ccccgaatcg
     8221 cgtgccagct ttacctgcgg aagaatgcgt tcgagcgaaa agaaatcttc ccaggaagaa
     8281 tgttttttat tggattgcgg gagcgagccg atgtaattgt catggccgag tccgaaaaat
     8341 ttttccgtgt gacggtggag cttcgcgagc tgcgttccgg cttcgtatga atttccgctt
     8401 cgcgaatgtt ccatccattc catcagcagg aaagaagaat cacgttcttc ccattgtgca
     8461 atgacgtgcg ggacaacgaa tgttttcgtt ccggccagca gctgcagtcc tttcgcttca
     8521 gcggcgaaca tgccgggaaa tttctgcgca tcgttctctt tcaggaaaaa ccagcctttg
     8581 tcggtttcga gacgaacgca ggaattgatg cttcctcctg tcaccggcga aacgcgttgc
     8641 aacgaaacat ggccatagcc gttctctgcc agccgccgga aatacccttt tattttttcg
     8701 gtctggccca tgccggtttc gtcttcatca cttttttata caccgggcag gaaggaatgt
     8761 gcgcaccggg aagatagccc gtgctcatga ggaattcgtt cacgatctcg ccgcccgtga
     8821 agcggaatgt ctttttaaag atcttcaccc actcttcttt tgttttcggg tgatgatgat
     8881 cgatccattg gcggaacgat ccgaattctt tctgcagcgc aagaatggca ttggcgttat
     8941 ggatcgtcgc ttcgatcttc aggcggttgc ggatgatgcc ttcgttgctc agcaggcggg
     9001 cgatctcgcg gcttttgtat ttcgccacgc gggaaatttc aaaattgtcg aatgcacgga
     9061 agaaattttc cttctttttc aggatggtcg tccagctcag ccccgcctgg ttgatctcga
     9121 gcacgagccg cgcgaaaagc tggttgtcgt catcgatcgg gaagccgtat tctttatcgt
     9181 ggtaatgaac atgcacattg cctttttcca tcttggaaac ggcttcgcag taggatctcg
     9241 gtttgacgtt ggccattggc gataatgaaa acgggaagga atgaaaaaag agaacggtcc
     9301 gcagcgtttg cgtgatctta ccggggttgc gcgcgttcga tgcggatgcc ggcgttcagt
     9361 acgcccggca gtttttccat gcgcatcgcc tgctcgactt ccatgtggta ttttccagcc
     9421 aatggaaaac ggacattctg ccggaagacg aggcgacagt cgtgcacatc gccctgtcct
     9481 tttcccgccc agcgcccgct ttcgtcgatc agcggaaaat caacggtgtc gttcgccatt
     9541 ttcccattgg gaaaagtggt gtggaagaaa acgtaaatgt tgcggtaagg ataatcttcg
     9601 ttgttgcgta tgttgaagca gaagttgtat ttcgtgattg tatccgggac atcgaagtcg
     9661 aacgagagac ggttattcat gtcccacgtg ttgttcggaa tgtcgcggtt ctcctcgaaa
     9721 aaacgcgtgc ggtcgcatga tgcgaaaaga agcatcgcgg cgaaagcgaa cgggagaagt
     9781 acggaataac gcatgatcag tttttcggtg cgggaggagt tcccggtttg tttccgccgc
     9841 ccggcctcgg gggacggttg cgccgctggt tccgctgggg attgtttttc tgctggttct
     9901 gctgctgcgg gttgttcggt cgcggctggt ttttctgttg ttgcggccgt ggctgcttct
     9961 gctgcggatt tttttgttgc ggcttttgct gcgtgttctg cggttgcccg ccggtgttcc
    10021 gctgaccgga aggttttctc ctttttttct ttttgctttt gtcaaaacgc gtgaggctgt
    10081 cctgcccgac gacattctcg aaatccggcg ctttctcgac agcttctttt ttatccttca
    10141 ccgcaccgat gtcttccggt ttcacgcctt ctttgttcag ctttatgatc tccttcacgc
    10201 gatcggtgga gagcggtatg aactggtcgg gctcatcggc gaagctgtac cacatgatgc
    10261 cgcggaaaat atccgtcttc tggtggcgtg cacgtccgcg gctggtttcg atgtcggcgg
    10321 tttccgggaa atcgcgtatg gcgtcgaggt aagaatcgag ctcgtagttg aggcagcatt
    10381 tcagcttgcc gcattgtccc gccagcttga gcgggttgag ggaaagctgc tggtaacgcg
    10441 ccgcgctggt cgtcacggag cggaaatcag aaagccacgt ggagcagcaa agttcgcgtc
    10501 cgcagctccc gatgccgccg agacgggccg cttcctgccg tgcgccgatc tgtcgcatct
    10561 cgatgcggat acggaactgc tcggcgagaa tttttatgag ctcgcggaaa tcgacgcgct
    10621 cttcggccgt gtaatagaaa atggctttcg tcttgtcgcc ctggaattcc acgtcgctga
    10681 tcttcatctg cagcccgagg cgctgcgcga tctcgcgtga tttcatcatc gtggattgct
    10741 cgagcgcctg cgcttctttc catttctcga cgtcattctc cttcgctttg cggtacactt
    10801 tccggatcgc ctggtcgtcg atcttcacgc cgcgccgcgc catctgtgtt ttcaccagct
    10861 cgcctgtaag cgaaaccgtg ccgatatcat gtcccggcga accttcgacg gcgatcactt
    10921 cccctgtcac aagcggaaga ttattgacgt tgcggaaaaa ttccttgcgg ctgttcttga
    10981 agcgaacttc caccacgtcg aaaggcgcct gcccggaagg cagctccata ttcgccagcc
    11041 agtcaaaaac attgagcttg ttgcagcccc cgatcccgca ggaaccgttg cttctgcaac
    11101 ctgcaggcac tccgttcacc actgtcccgc atcctctgcc cgacgaacaa ccgctgcatc
    11161 ccattttata tgaagttcaa aaaaggttcg atttaaaaaa actgttccgg aagattccgg
    11221 aggggtaaag acaaaaatag cagtttaaac ggaagaaggt gctcacaccg cctgcggctt
    11281 cgggatgttc agcaattcgt tcgctttgaa ggaaaggtcg gtgaagatga tcttcgggct
    11341 gccgttgcgt tcgatgtgct gtatcgcttt gctgagctcg ttcatgaatg cttcgccgtt
    11401 cgccgcgtga atgaacggcg agaatttccg gatgaacgcc agctcttcgc ccgtcatgcg
    11461 caccaatgac ggatcggcgt aattgatcag caggcattcg cggacaagcc cgagcgcata
    11521 ggaaaggaat tgcttctgac gttcccggcc gataccggca aggtcgtcta ccgcgctcag
    11581 cactttggcg acatcgaact tcagcgaggc ccgcatccac tgcgtgaacg tggaaagatt
    11641 gaacgcggcc tgctcgctgc ggctgatcag gctgcgcgct tcggcgtaat ttccttcggc
    11701 ctggaaggcg attttttctg cgtccgccgg tgaaagcgaa tggcgctgca caagcgcatc
    11761 gcgcaggtct gcatccgcga tcgcgtgtat gcggacaagc tgcgtgcgtg aaacgatcgt
    11821 gcgcaggagc tggtcttcat tttcggtaac gacgaggaac agcgttttgt cgggcggctc
    11881 ttcgagaatt ttcagcagct tgttcgccga tgccgcattc attttttccg ccagccacac
    11941 gatcatgatc ttgtattctc cttcgaaggt ggtcaggctg agcttgcgca ggatctcgcc
    12001 gctttcttcc gtcccgatga tggcctgctt gttttccgct tccagcttcg tgaaccattc
    12061 gtcgagcgtg aggtaaggat tttcgaggaa tgcttcgcgc cattccgcga tcacgtgcgt
    12121 acttacgcga acgtcctttg aaagtgcaat gggaaaaacg aaatgaagat cagggtgaac
    12181 gagacgcgcg tacttcacgc aggacgggca ttctccgcag gagtcgttct ccttccggtt
    12241 cgtgcaatga acatattgcg cgtaagccag cgccagcggc agcgcaccgc ttcccgggtg
    12301 accgaagaac agctgagcgt ggctgatccg ctgcgatcgt acggaattca ccaggcgttc
    12361 cctggtatcc cgctgcccga taatttcctg gaaaagcatc gcgtaaaagt aacaaagtgc
    12421 gattcatttt tcaggaaaga aaatgatgtt gctgagacag cgtcgccgaa aacgcccgcc
    12481 ccttttttct gaaaagggct gttccggccc cgaacttttt gttgcatatt ttgctgattt
    12541 gaagaacgtt gcttgacatt ctggtagtcc gaacctaatt tgcttttgca aacacccttc
    12601 tgacccatga aaaaaagcgt tacccgtgtc ctttacggac tactgttttt ttgctgcgcg
    12661 aaactttctg cgcagatgaa tgtaaacggc gggatccccg tacacaccaa catttccggc
    12721 ggccaggacc aggtcgatct ctgcgtgaca aacgttcatg acgggatcat cttcgtctgg
    12781 cgtgatagcc gttccggctc caacggccag gatatttatg cgcagaagct gacgccctgc
    12841 ggagacgtag cgcccgggtg gcccgcatcg ggattgctcg tctgcaatgc ggcaggcaac
    12901 cagcaggtgc cgcagatcgt tcccgataca tccggcggtg ccgttattac ctggcaggat
    12961 acgcgcggag ccgactggga catttatgca cagcatgttt cttccgccgg cgtttgtaca
    13021 tggacggcca atggcgtcgc ggtgtgcacc gccaccggca accagcagaa tcctcacctg
    13081 tgcatcgacg ggacaggcgg agccgtaatc tgctgggacg atatgcgcag cggcaactgg
    13141 gacgtttatg ccgcaaaagt gacgggatcg ggaacaactc cctggaccgc caacggagtt
    13201 ccgctcgtat cgctgagcgg aacgcagaac aacaactgca tcgcgacaga cagcgcaggc
    13261 ggcgcgttct tcgcatggga agacagccgt cccgcttcga acggcatcga cctctacgcg
    13321 attcgcctga acactaatgg caatgttcct ggcggatggc ccgccaacgg tcttgcgctt
    13381 tccacgcaga cctcgaacca gaaagagccc tgcgcatgca acaccggtgg tcagaccgcg
    13441 atgatcgcgt gggcggatgg tttcggcgca ggacaatcga tcctggcatc acgactcaat
    13501 ggcagcggcg gatatgtcgc cggctggagc tacccgggaa accttgtcgg caacgcgatc
    13561 acgcagagcg gcaaccccgg aacagcggag ctgcccaaaa tcgctcccga ctgcggcggc
    13621 ggcgcttaca tctcgttcgg aagccattat tcatcgagcg accacgacgt gcttacccag
    13681 cacatgggat tcaacggcat tccgagcggc ggccttgaaa caacggcttt caatacagac
    13741 atagaacatt accaggagat ctgcgccgac gggaacggag gtgcggtgaa tgtgtggcag
    13801 gaccagtggc cgccggctat tgtctggtac aggaaattcg gcagcggcgg agtgatcgcc
    13861 agccagttcg tgagcagcac ttaccccgat gtggctgcga tgcgcggagt atggtatgct
    13921 gcctgggaaa acaacggcgg tgccgcagtc atcggttcga gcgatatcta tgcgatatct
    13981 tccagcgcaa gcgcacctcc cccgatactg aattccacgc ttacgcaaac gtataccggg
    14041 acgtcgtgtg atatcaccac gcaaaacgtt tcgctttgcg ccgggtctcc cattacattt
    14101 tccgttccgc tgccgagggg attatacaac acctacactt tcctcgacgg agcgactgtt
    14161 ctgcaaacgg gaaccagcgc atcatacacg acgtcggcat tgaccgtggg aacgcacacc
    14221 atcaccgcca cgtcttctat cagcggaagc tgcgtaaccg cggcaagcaa ccagatcgtt
    14281 gtcaccgtca ccgcaggacc ggccgttacg ctttcttccc agacggacgt tctctgcaat
    14341 ggcggcagca caggttcggc aaccatgaat gcgaccggcg gatcgccggc ttacacgtac
    14401 gcgtggacac ccgcgggcgg aaatgccgcg acggcgtcct cgcttgcggc gggaacctac
    14461 acctgcctcg ttacagatca gctgggttgt gcgcagacac aaacagtgac gatcacggaa
    14521 ccggcagcga tcacggcaac cgtttcttca acggcggcta cctgcggcaa caccaacggc
    14581 tcggccaccg tgaatccggc aggtggaaac ggaagttatt cctacgtgtg gtcgccttcg
    14641 ggcggtaatg cttccacggc atcttcactt gcggcgggaa gctacacctg tgccgttacc
    14701 gatggaaacg ggtgttctca gaattttcct gtttccgtaa acagcaccgg aggtccgact
    14761 gttactccgg cttcccaggc tgacgtcagt tgcttcggcg gggcaaacgg cacggcttcc
    14821 gttaccgtga cgggcggaac acccggttac tcgtatgcgt ggacgccgtc cggtggaaat
    14881 gcggcgaatg cttcaggact tggtgcaggt acgtacacat gcacggttac cgacggactc
    14941 ggatgtgttc aatcgcaaac gttcagcatc acgcaaccga ccgcattgag cgcaacatct
    15001 tctgtaacga atgtcgcatg caacggaggt ttgaccggca gcgcaacagt gaatccttcc
    15061 ggcggtgcag gcggttattc t
//
LOCUS       DASTSF010000621         4973 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1473195,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000621 DASTSF010000000
VERSION     DASTSF010000621.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4973)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4973)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4973
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1473195"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1317)
                     /locus_tag="VFU15_17485"
     CDS             complement(<1..1317)
                     /locus_tag="VFU15_17485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719643.1"
                     /translation="MTSHSMLLSGLSAGMTYYYRVTSVDGTMDTATVPAPPAAPLSFI
                     MPSSFCAQDELNADFSAGTDTNTVVTQVADGEVRLQPLLNEDFAGTGVPAGWTDEIWD
                     AQAGAVTTYNGGSVSVNGTHIYTNTAYGPSASVEFSATFTPGNFQNVGFTADPAFNSP
                     WVVIGQGVSDGNIYARTSNNETVLLGAGLLNTPHVYRIQRNPSTGGFSFYVDGTLIAT
                     PGIALTTTANLYVQVSDYPAGGAALTVDWLRITPYVSAGTFTSRIFDHSDTTTWGNVI
                     WTDSIPAGTGLSVYARGGNTSSPDATWSPYLALVNGSPVGITSRYLQYKADLTTSDST
                     VTPALKDIGFECSTGPGSCVPPLAYISAVDTSLCQGDAIQLRLDSATGASPYSLVVNG
                     VAYTNVTPGQVFATLSTPATSLWGSGGSPANPNGNDGQPIEVGVKFQ"
     gene            complement(1522..>4973)
                     /locus_tag="VFU15_17490"
     CDS             complement(1522..>4973)
                     /locus_tag="VFU15_17490"
                     /inference="COORDINATES: protein motif:HMM:NF042648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4082 domain-containing protein"
                     /protein_id="HEU4719644.1"
                     /translation="WENSTDVSNTPYRTLVCYKEGDLGENVCGTKCDPLPNVWTGLWR
                     NGCAYPAADGCNPENSLSGQISWDGTTGAIEVPDTYKNLRFWRNTSIASLGTGQTATL
                     SAGTLGYEWDWNQYTSSYPAGRISMSRTVLNSHTHNLSLYRASSGALVFGAGTVQWSW
                     GLDGNHDRGGSVEDVRMQQATVNLFADMGVQPATLQAGLVPAIASTDVQAPVSVITSP
                     LNGDSLTNGVQVTISGTANDNILVAGVEVSVDGGATWHAASGTTAWSYSWTPLIAGPV
                     NIRSRAFDDSGNLENPGAGITDTVSLPLPVVCPCSIFQPADTPAVQLANDGQSIEFGV
                     KFQSAIAGYITGLRFYKGNGTTGTHEGHLWTGTGTLLADETYTSETSNGWQLVTLSTP
                     VAINANTTYVASCYSSSGDYPYTDLFFSNAFTHGPLQALADGEDGPNAVYAYGYGPTF
                     PENSYQSSNYWVDVVFADSVGPDTIPPVVSATSPANGATNVATGTSLTINFSESADPS
                     TVNTSTIELRDASNALVPSIVSYNAATHSATLIPNAPLAYLETYTATVKGGTGYYRVK
                     DIAGNAMIADHTWSFTTAPAPPPFPAQGPGGPILLIGSSLNPFSLYPVEILRAEGFNE
                     FTAMDIADVNATVLNAYHVVIVGEIALTSPEVAMLTNWTNAGGTLIAFRPDAQLAPLM
                     GITPTGNTLSDQYLMVNTATSPGAGITAQTMQFHGTADLYTLSGAAAAATLYSDATTP
                     TAYPAVTINNVGTNGGQAVAFTYDLARSVVYTRQGNPAWAGQKRDGQTGPIRSDDQFF
                     PNYIDMNKVAIPQADEQMHLLSNIILSGNMDHEPLPRFWFLPKGLKAAIVMTGDNHGD
                     YGMQPRFDQDIAQSTPGCSVNDWECIRSTGYLYVGSIFTDSMARHYDSLGFEVALHVN
                     TNCSNFSAFQYNSFVTTQLGGFQAAFPSVPSPSTNRNHCIAWSDWSTVPETEAANGIR
                     FDANYYYWPDTWVNNRPGMFTGSGMPMRFAKLDGSLIDCYQAPTQMTDESGISLPGFC
                     DSLLDKANGPQGYYGVFVTNMHFDQANHPGANAIVASAQAHHVPVVSAKQMLDWIDAR
                     NNSSFGSITWNGDTLDFTVSADTSARNMKAMIPVHVNTKTLISVTFNGTPVAYTQEII
                     KGI"
BASE COUNT         1104 a         1350 c         1353 g         1166 t
ORIGIN      
        1 ttggaatttc acgccgacct cgatcggttg cccgtcattc ccgttcggat ttgcaggaga
       61 accgccgctt ccccacaagc tcgtcgcggg agtggagaga gtggcgaaga cctgcccggg
      121 tgttacgttc gtgtaagcga cgccgttcac caccagtgaa taaggtgatg cgccggtggc
      181 gctgtcgagg cgcagctgga tcgcatcgcc ctggcagagc gaagtgtcga cagcggaaat
      241 atacgcgagc ggcggcacgc acgagcccgg acccgttgag cattcaaagc cgatgtcttt
      301 cagcgccggg gttaccgtgc tgtccgatgt ggtaagatct gctttgtatt gcaaatacct
      361 ggacgtgatt cccacgggcg atccgttcac aagagcgagg tacggcgacc atgtagcgtc
      421 aggcgatgat gtatttcctc cacgcgcata cacggataaa ccggttccgg cgggaatgct
      481 gtcggtccag atcacatttc cccacgtcgt cgtatcgctg tgatcgaaga tgcgtgatgt
      541 gaatgttcct gcggaaacat aaggtgtaat tctcagccag tcgaccgtca gcgcagctcc
      601 gcctgctgga tagtcgctca cctgtacata aaggttcgcg gtcgtggtca gcgcaattcc
      661 cggcgtggcg atcaacgttc catcgacgta gaaagaaaaa ccaccggtgg atgggttacg
      721 ctggatgcga tatacatgcg gcgtattcag aagcccggca ccaagaagaa ctgtttcatt
      781 gttcgatgtc cgcgcataaa tatttccgtc gcttactccc tgcccgatca cgacccacgg
      841 actgttgaac gcagggtctg ccgtaaagcc gacattctga aaattacccg gcgtaaacgt
      901 agccgaaaat tcaacggaag ctgaaggacc atacgctgta ttagtataga tgtgcgttcc
      961 attcacgctg acgcttcctc cgttataagt agtcactgct cctgcctggg cgtcccagat
     1021 ctcatctgtc catcctgcgg gaacgccggt gccggcaaaa tcttcattca gcaatggctg
     1081 cagcctcact tcgccgtccg ctacttgcgt taccaccgta ttcgtatctg ttccggcaga
     1141 aaaatctgca ttcagctcat cctgcgcaca gaaagaagaa ggcatgataa agctaagtgg
     1201 cgcagcagga ggagccggca ccgtcgcagt atccatcgtt ccgtcaacgg aggtgacgcg
     1261 ataataataa gtcatgcccg cggaaagtcc gctgagcagc atggaatgac tggtcacggg
     1321 cgagctgttg tttgcatgcc gtgtcagcgc cgatgctgtc gtgtcgtaat ctgtttttga
     1381 atttgccgcg atgttggtgt tccacgcaat tacagccgtt ccgttgagtc ccggcgtggc
     1441 tgttacattc gaaatgacaa gcgccggtgc tatatcgtat atcgcttcat agttcccgct
     1501 ttgcgccggg atgaatgcat attatattcc tttgatgatc tcctgcgtgt aagcgaccgg
     1561 tgtgccgttg aatgttacgc tgatcagtgt tttagtattc acatgcaccg gtatcatcgc
     1621 tttcatgttg cgcgcactcg tatcggcgct tactgtaaaa tcgagcgtgt cgccgttcca
     1681 cgtgatggaa ccgaaggaag aattgttccg cgcatcgatc cagtcgagca tctgctttgc
     1741 ggaaacaaca ggaacatgat gcgcctgcgc cgaagcgacg atcgcatttg caccgggatg
     1801 gttcgcctgg tcgaaatgca tattggtgac gaatacaccg taatatccct gcggaccatt
     1861 tgctttatcc agcagcgaat cacagaagcc cggcaatgaa attcctgatt cgtccgtcat
     1921 ctgcgtcggc gcctggtaac aatcgataag actgccatcg agctttgcaa aacgcatggg
     1981 cattcctgat cccgtaaaca tcccggggcg attgttcacc cacgtatccg gccagtaata
     2041 atagttggcg tcgaaacgta tcccgttggc tgcttctgtt tcaggcacgg tgctccagtc
     2101 gctccacgca atgcaatgat tgcggttggt cgaaggtgaa ggaacggaag ggaacgccgc
     2161 ctggaaaccg ccgagctgcg tcgtcacgaa tgaattgtat tgaaatgcgc tgaagttcga
     2221 acagttcgta ttcacatgca gcgcaacttc aaatccaaga ctatcataat gacgcgccat
     2281 cgaatcggtg aatattgatc ctacatacag gtaaccggta gaacggatgc attcccagtc
     2341 attcaccgaa cagcccggtg tgctctgcgc gatatcctgg tcgaaacgcg gctgcatacc
     2401 gtaatctcca tgattgtcac cggtcattac gatcgccgct tttaatcctt tcggcaaaaa
     2461 ccagaaacgc ggaagcggtt catggtccat atttccgctg agaataatat tcgaaagcaa
     2521 atgcatctgt tcatccgcct gcgggatcgc cactttattc atatcaatat aattcgggaa
     2581 gaactgatcg tcggaacgga tgggaccagt ttgcccgtcg cgtttttgtc ccgcccacgc
     2641 cgggtttccc tggcgcgtat agacaactga gcgcgcgaga tcatacgtaa atgcaacggc
     2701 ctgtcctccg ttcgttccca cgttatttat tgtgactgcc ggataagctg tcggtgtggt
     2761 tgcatcagaa tacaacgtgg ctgctgctgc tgcaccggaa agcgtgtaga ggtccgctgt
     2821 gccgtgaaac tgcatggtct gcgcagtgat cccggcgccc ggtgatgtcg ctgtgttcac
     2881 catcaggtac tgatcggaaa gcgtgttccc ggtcggtgta attcccatca gcggcgcgag
     2941 ctgtgcatcg ggacgaaatg cgatcagcgt tccgcctgca ttcgtccagt tcgtcagcat
     3001 ggcaacttca ggagaagtca gcgcaatctc cccgacgatc actacgtgat acgcgttaag
     3061 caccgtcgca ttcacatcgg caatatccat cgcggtgaat tcattgaatc cttccgcacg
     3121 aagaatttca accgggtaga gactgaacgg gttcagcgaa cttccgatca gcagtatcgg
     3181 tccgccgggt ccctgcgcgg gaaaaggcgg cggggccggg gcggttgtga atgaccatgt
     3241 atgatcagcg atcatcgcat tgccggcaat atcctttacg cggtaataac ctgtaccgcc
     3301 ttttaccgtt gctgtatacg tttccaagta ggcaagcggt gcattgggaa taagtgtggc
     3361 gctgtgcgtg gctgcattat aggaaacaat ggaaggcacg agcgcgttcg atgcatcccg
     3421 cagctcgatc gtcgatgtgt tcaccgtcga aggatctgcg ctttcactga aatttatggt
     3481 aagcgaagtt ccggtcgcca cgtttgtcgc tccgttcgcg ggagatgttg cgctcactac
     3541 cggcgggatc gtgtccggtc cgacgctgtc tgcaaataca acatccaccc agtaattgct
     3601 cgactggtag gaattctccg ggaatgtcgg tccgtaaccg taggcataaa ctgcgttggg
     3661 tccgtcttct ccgtcggcaa gcgcctggag cggtccgtgc gtaaacgcat tgctgaaaaa
     3721 cagatcggtg tacgggtaat cgccggagct gctgtagcag gatgcgacgt atgtcgtatt
     3781 cgcgttaatg gcaacaggag tgctcagcgt aacaagctgc cacccgttgc tggtttcgga
     3841 agtgtaggtt tcatcggcga gcagtgtgcc tgtacccgtc cacaaatgac cttcatgcgt
     3901 acctgtcgtt ccgtttcctt tgtaaaaacg gagaccggta atatatcctg cgatggcaga
     3961 ttgaaacttc acgccgaact caatggattg tccgtcattc gccagctgaa ccgcaggcgt
     4021 atccgccggc tggaaaatgc tgcagggaca aaccaccggt aaaggaagcg aaaccgtatc
     4081 ggtaattccc gccccgggat tttcaagatt tcctgaatca tcgaacgcac ggctcctgat
     4141 gttcacgggt ccggcgatga gcggcgtcca gctgtatgac catgcagttg ttcccgaagc
     4201 tgcgtgccac gttgcaccgc catcgacaga aacttccacg cccgctacaa ggatattatc
     4261 attggcggtt cctgaaatgg tcacctgcac gccgttcgtc aatgaatctc cattgagcgg
     4321 ggaagtgatc accgaaacag gcgcctgcac atccgtcgat gcgatggcgg gaacaagtcc
     4381 tgcctgcaac gtcgccggct gcacgcccat atcggcgaaa agatttaccg tcgcctgctg
     4441 catgcgcaca tcttctaccg aaccgccgcg gtcgtgattg ccgtccagtc cccacgacca
     4501 ttgcacggtc cccgcgccga atacaagcgc gccgcttgac gcacggtata atgacaaatt
     4561 atgcgtatgt gaattcaaca ctgtccgcga catgctgatg cgacctgcgg gataggaaga
     4621 tgtatactgg ttccagtccc attcgtaacc gagcgtaccc gcagaaagtg ttgcggtctg
     4681 gccggtaccg agtgatgcga tcgatgtgtt gcgccagaag cgaagatttt tgtacgtgtc
     4741 cggaacttcg atggcgcccg tcgttccgtc ccagctgatc tgcccgctta atgaattttc
     4801 aggattgcat ccgtcggcag cgggataggc gcagccgttg cgccacaagc cggtccaaac
     4861 attggggagc ggatcgcatt tggttccgca gacattttca ccgagatcac cttctttata
     4921 gcagaccaaa gtacggtacg gtgtattgga aacatccgtg ctgttttccc agc
//
LOCUS       DASTSF010000622         5617 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1025652,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000622 DASTSF010000000
VERSION     DASTSF010000622.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5617
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1025652"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            61..5283
                     /locus_tag="VFU15_17495"
     CDS             61..5283
                     /locus_tag="VFU15_17495"
                     /inference="COORDINATES: protein motif:HMM:NF039936.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HEU4719645.1"
                     /translation="MNASSGSSITVTGLSPGTWYDFNVFDYDDDAPTYYNYRSELMCT
                     GEVQTGAGAVPVITNMSPATGPVGTVVTLTGSNFGSTVYNNIVYFGATRATVTAASAT
                     SLTVVVPYGANYNPVSVQVDGLTGTYSKEFIVTSSCAATINASSFTNSSVTTSGPTIG
                     NVIKDIDNDGKSDFMVQDYGGSVFNAFRGTSSNGTTPPAFTANTYGTVTNPYFMECAD
                     FDLDSKPDVAMTDYIGGKVSIFRGNGTYFSTATRFDFATLPYPAMVKSGDIDGDGRPD
                     LVVSYGTGTYISVFRNTGSITNIDFAARQDFNIGTNTPFSLVVRDFDLDGKADIAVGQ
                     SGGTNFYAMRSTSTPGSVTFAAVQAFSAGTGNVNGLTTGDFNLDGKADIAVALSNNTV
                     RLYGNNSTVGSISFSYANQLTTASSNPIALASNDLDGDGKIDLAVGYSSANTVSVFEA
                     TGPYGFATRVDYATAGSESACLSIGDFNQDGKSDILASTFGSSVNILSNAMNALASEP
                     PSTASNLTFSGVTQSSITLNFTPGTGANRIVVCRQGGAVTILPADGTTYTGNSVFGSG
                     TDVGGGEYCVYNGNSNSVTVTNLQSNTYYYFAIFEYNGSTCTTNYLTAPYASSNTQTL
                     NTPPTLSAISNPPAICQNSGQQTVNFSGVGTGAANETQTLTVTATSNNTSVIPNPTVT
                     YTSPNATGSLSYTPVPSATGSATITVTVNDGASNNATTTQTFVVTVSPPPTPSNAGPD
                     QQICTGTATLGANAPSVGTGVWSIAYTSNVSITTANLGNVNIPTTTLTGLTVGDSVRL
                     AWTITSSPCNPSVSYVSIKRTNCPLTAGFTWSPQNLCATALQVNNISFTDASFAPSTT
                     ITSWSWSFTGPIAPSPSNSSQQNPSNIQFTGPGTFTVTLTINDNVGGNSTTTQFITIN
                     QYPAGSGTISGPSTVCQGQTGVTYSVLPIAYATSYNWALPPGATIVNGAGTPTIIVDF
                     DSNASSGSVQVQGVNSCGAGAASPIYNVTVNPLPVNTSSVNGPSSVCQGQNNVMFTTP
                     SIPYATGYTWTLPSGATIIGNPSNDTIYVNFSGSAQSGTVDVIGTNSCGAGTSSVGYM
                     LNVNPLPDSAGTITTNQTGIICQGQTGVTYSISALNNSNYYEWTVPAGATIVSGDSTS
                     SITVDYGSSAVSGNVTVSGVNSCGNGTSSSLAVTVAPLPAPASSVLGQDTVCQGEFSV
                     SYFVSAISNSTSYVWSIPGGSSIATGQNTNSITVDFGPGATNGQIVVYGQNVCGTGTP
                     SAPLDVVVNPLPDSGRTVTGPSAVCQGDSAVTFSVNTIANATGYDWHLPPGANITAGN
                     NTNSITVDFSTTAISGPVYVTGTNACGMGATSDTLNLIVNPLPDPAGTMSGPATLAIC
                     PISTGIVFSVPPVNNASYYVWSLPPGATIVSGDSTNSITVDFAPGASPGAATVYAQNA
                     CGTGAPSQLSFNFDQVTPVDICEVTVDGNSVYNHVIWNKPVATDIDSFRVYREITANN
                     YVHIGSVPYDSLSVYVDSVYVPTADPNTTYFRYELSSVDSCGNESPLCAHHRTLFLQA
                     NVGLGNTVNLNWSLYEGATVDYYRILRDSTGMGNWQVIDSVAGTTNLYTDQNPPMSVN
                     VVDIRYKLQTVWALSCSPSRNIVTSESNLKDVPIAAFSVHENVLDQLVNVFPNPSDGN
                     FTVQCPYSSSGYVISVYDELGQLVYVYKMEAKNMNPGENTFSLPLSTLAKGTYYMTME
                     DGNNQPMHKKLVIQ"
     gene            5424..>5617
                     /locus_tag="VFU15_17500"
     CDS             5424..>5617
                     /locus_tag="VFU15_17500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS/SEC14 domain-containing protein"
                     /protein_id="HEU4719646.1"
                     /translation="MQPPTDRKTYEGEIATYWIDDDGILVSLSKSTLRTVENITGNIA
                     LVKSITGNRKLPLLIYLSSSP"
BASE COUNT         1327 a         1649 c         1397 g         1244 t
ORIGIN      
        1 tactacccaa gctctacatt cggaaacgga agcgtcgtcg gaacaccgta ttacgccgtg
       61 atgaatgcct ccagcggaag ctcgatcacc gttacgggat tgtcgccggg aacctggtat
      121 gatttcaatg tatttgacta cgatgatgac gcaccgacct actacaatta cagatcggaa
      181 cttatgtgca caggtgaagt tcagaccggt gcaggcgcgg ttcccgtaat taccaatatg
      241 tcgccggcaa caggacccgt cggaactgtc gttacgctga cgggatcgaa cttcggttcc
      301 actgtttaca acaacattgt gtatttcggc gcaacaagag cgacggttac ggcggcgagc
      361 gcgacatcgc tgaccgttgt ggttccctat ggcgccaatt acaacccggt ttccgtacag
      421 gttgacggat tgaccggaac ctattcgaaa gaattcatcg tcacttcttc ctgcgcggca
      481 acgatcaacg ccagctcgtt cacgaactct tcggtaacga ccagcggtcc tacgatcggc
      541 aacgtgatca aggacatcga taacgacggg aaatccgact tcatggtgca ggattacggc
      601 ggcagcgttt tcaatgcttt caggggaact tccagcaacg gaaccacgcc tcccgcattt
      661 acagcgaaca cctacggaac cgtaacgaat ccctatttca tggaatgcgc cgatttcgat
      721 ctcgattcga aacccgatgt cgccatgacc gattacatcg gcggaaaggt ttccattttc
      781 agagggaacg gcacttattt ctcgaccgca acgcgttttg atttcgcaac gcttccttat
      841 cctgcgatgg tgaaatcagg agacatcgac ggtgacggcc gcccggatct tgtcgtcagc
      901 tatggcaccg gaacgtatat ttccgtgttc cggaatacgg gctccattac aaatattgat
      961 tttgccgccc ggcaggattt caacatcgga acgaacacgc cgttctctct tgttgtgcgt
     1021 gatttcgatc tcgacggaaa agcggatatc gctgtcggtc aatcgggcgg aacgaatttc
     1081 tatgcgatgc gcagcaccag cacaccgggt tccgtcacgt tcgctgccgt gcaggcattc
     1141 tctgccggca ccgggaacgt caatggacta acgaccggtg atttcaacct cgacgggaaa
     1201 gcggatatcg ccgttgcgct ttcgaacaac acggtccgtc tttacggcaa caattcaacc
     1261 gtcggctcca tttcattctc ttatgccaac cagctgacga cggcgagttc caatcctatc
     1321 gcccttgcca gcaatgatct tgacggcgac ggaaaaattg atctcgctgt aggttattcc
     1381 agcgcgaaca ccgtttctgt tttcgaagcg acaggtccgt acggattcgc cacgcgcgtc
     1441 gattatgcga ccgcaggttc ggaatcggcc tgcctcagca tcggcgattt taaccaggac
     1501 ggaaaatcgg atattctcgc ctcgaccttc ggatcaagcg tcaatattct ttcgaatgca
     1561 atgaacgcgc ttgcttcgga accgccgagc actgcttcca acctcacgtt ctcgggcgtt
     1621 acgcaaagtt ctatcacgct taatttcaca cccggtaccg gcgcaaaccg tatcgtcgtg
     1681 tgcaggcagg gtggcgccgt cactattctt cccgctgacg gaacaaccta cacgggcaat
     1741 tccgttttcg gttccggaac ggatgtcggc ggtggagaat attgcgttta caacggcaac
     1801 agcaattctg ttacggtcac caacctccag tcgaacacgt attattattt cgcgatcttc
     1861 gaatacaacg gatcgacgtg cacgacgaat tacctgaccg cgccgtacgc ttcttcgaat
     1921 acacaaacac tgaatacacc accgaccttg tcggctatca gcaatccgcc ggcgatctgc
     1981 cagaattccg gccagcagac ggtgaacttc tccggtgtcg gaacgggtgc agcaaatgaa
     2041 acacaaacgc tcaccgtcac cgccacgtcg aacaacactt ccgttatccc gaacccgacc
     2101 gtgacgtaca catctccgaa cgccaccggt tcgctctcgt acacgcctgt accgagcgca
     2161 acaggatccg caacgatcac cgttacggtg aatgacggtg cttcgaataa cgcaacaact
     2221 acgcagacct tcgtggttac ggtgagtccg ccaccgacgc cttcgaacgc cggacctgat
     2281 cagcagatct gtacaggaac ggcgacgctc ggtgcaaatg cgccttcggt cggaacaggt
     2341 gtatggtcca tcgcttacac gtcgaatgtt tccataacta cggccaatct cggcaacgtg
     2401 aacattccga caacgacgct tacaggtttg acagtcggcg attcggtccg ccttgcatgg
     2461 acgatcacca gctcgccgtg caatccttcc gtcagctacg tgagcatcaa gcgcacgaac
     2521 tgtccgctca ccgccggatt cacgtggtcg ccgcaaaacc tttgcgcaac cgccttgcag
     2581 gtgaacaaca tttcattcac cgatgcttct ttcgcaccca gcacaacgat cacttcttgg
     2641 agctggtcgt tcaccgggcc gatcgcacct tcaccgtcca actcgagcca gcagaacccg
     2701 tcgaacatcc agttcactgg tcccggaaca tttaccgtca cgctgacgat caatgataac
     2761 gtgggcggaa acagcacgac gacgcagttc attacgatca accaatatcc cgctggttcc
     2821 ggcacgatct ccggaccgtc cacggtttgc cagggacaga caggcgtaac ttattccgtt
     2881 ctcccgatcg cctatgccac cagctacaac tgggcactgc cgccgggcgc aaccatcgtg
     2941 aacggagcag gcaccccgac gatcattgtt gatttcgact ccaacgcgtc ttccggttcc
     3001 gtgcaggtcc agggcgtgaa cagctgcggc gcaggtgcag catcaccgat ctacaacgtc
     3061 acggtaaatc cgctgccggt gaacacaagc tccgtcaacg gtccgtcttc cgtctgccag
     3121 ggacagaaca acgttatgtt cactacacct tcaatacctt acgcaaccgg ctacacgtgg
     3181 acattgcctt ccggcgcaac cattatcggg aacccgagca atgatacgat ctacgtcaat
     3241 ttctcgggct ccgcacaaag cggaacggtg gatgtgatcg gaacaaacag ctgcggtgca
     3301 ggaacttctt ccgtcggcta tatgctgaat gtgaatcctc ttcctgactc ggcgggcacg
     3361 atcaccacta accagaccgg cattatctgc cagggacaaa ccggtgtcac ctattctatc
     3421 tctgcactga acaattccaa ttattacgaa tggactgttc cggctggtgc aacgatcgtc
     3481 agcggtgaca gcacatcatc gatcaccgtc gattacggtt cttcagccgt ttcaggcaac
     3541 gtcaccgtgt ccggtgtgaa cagctgcgga aacggaacat cgtcttcact cgctgttaca
     3601 gtcgcaccgc ttcccgcgcc tgcatcttca gtgctcggac aggacacggt gtgccagggt
     3661 gagttcagcg tgagctattt cgtaagcgcg atctcaaatt caacttccta tgtgtggtcg
     3721 atcccgggcg gttcctcgat tgccacggga caaaacacga actcgatcac cgtcgacttc
     3781 ggtccgggcg caacgaacgg acaaattgtt gtgtacggac aaaacgtttg cggaaccgga
     3841 actccttccg ccccgctcga cgttgtcgtc aacccgcttc cggacagcgg cagaaccgtc
     3901 accggaccgt ccgctgtatg ccagggagat tccgctgtta cgttcagcgt gaacaccatt
     3961 gccaatgcaa ccggctacga ctggcatctt ccgccgggag cgaacatcac tgccggcaac
     4021 aacacgaaca gcatcacggt cgatttcagc acgacggcga tctccggccc ggtttacgtg
     4081 acgggaacga acgcgtgcgg aatgggcgca acatcagata cgctcaatct tattgtgaat
     4141 ccgctgcccg atcctgcagg aaccatgagc ggacctgcca cgctcgcgat ctgcccgatc
     4201 tcaacaggaa ttgttttctc cgttccgccc gtgaataatg cgtcgtatta cgtctggtca
     4261 ttgcctcccg gagcgacgat cgtttccggc gacagtacga acagcatcac ggttgatttc
     4321 gcaccgggcg caagtccggg tgcggcaaca gtctatgcgc agaacgcctg cggaaccggc
     4381 gcaccttcac agctcagctt caatttcgac caggtaacgc cggtggacat ctgcgaagtg
     4441 acggttgacg gaaactccgt ttacaaccac gtgatctgga acaaaccggt ggcgacggac
     4501 attgattcgt tccgcgttta tcgcgagatc actgcgaaca actacgtgca catcggttct
     4561 gttccgtatg attcgctcag cgtttatgtc gattcggttt atgttccgac tgcagatccg
     4621 aacaccacgt atttccgtta cgagctttct tccgtcgatt cgtgcggaaa tgaaagtccg
     4681 ctctgcgcgc atcaccgcac gctgttcctg caggcgaacg tcggtctcgg aaatacggtc
     4741 aacctgaact ggagtcttta tgaaggtgcg accgttgatt attaccgcat cctccgcgac
     4801 agcacgggca tgggcaactg gcaggtgatt gattccgttg cgggaacaac caatctttac
     4861 accgaccaga acccgccgat gtcggtgaac gtggtggaca tccgctacaa gctgcagacg
     4921 gtatgggcgc tcagctgcag cccgtcgaga aacattgtaa cctccgaatc gaacctgaaa
     4981 gatgttccga tcgctgcatt ctctgttcat gaaaacgtgc tcgaccagtt ggtgaatgtt
     5041 ttcccgaacc cgtctgacgg aaatttcacg gtgcaatgcc cgtattcttc ttccggctat
     5101 gtgatctcgg tttatgacga gctcggacag ctggtgtacg tttacaaaat ggaagcgaaa
     5161 aacatgaacc cgggagaaaa cacattcagt ctcccgcttt caacgcttgc gaaaggaacg
     5221 tattacatga cgatggaaga cggaaacaac cagccgatgc acaagaagct ggtgatacag
     5281 taagttttca ttgaaagatc cagaccccgg cccgtcatgt gccggggttt tttagttata
     5341 gaataccagg taaacgcaaa tgttctcctg gcatctgcgt tacagtacct ttcaacaaag
     5401 taattttgct tttagaaaaa aacatgcagc cacccaccga ccgcaaaaca tacgaaggag
     5461 agatcgcgac gtactggatc gacgatgacg gtattctcgt ttcactttcc aaaagcacac
     5521 tgcgcacggt ggagaacatt accgggaaca tcgcgctggt aaaaagcatt accgggaaca
     5581 ggaaacttcc gttgctgatc tacctcagca gttcccc
//
LOCUS       DASTSF010000623         4123 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_425921,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000623 DASTSF010000000
VERSION     DASTSF010000623.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4123
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_425921"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..608)
                     /locus_tag="VFU15_17505"
     CDS             complement(<1..608)
                     /locus_tag="VFU15_17505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719647.1"
                     /translation="MKPQWKIIMLITALCITRILSAQQADRSAFRPASATNAGTTVKP
                     DFRPAFLAYDYDDYDLAKHKRRKKHKGGGRGRDQAFGQGKFVITAGYGFPNLTKAVFK
                     AYESNLGFKVSGFGPMHVKFDYGVTDKLSLGLSIRYLSYKVQWTDEESITTYDSLGNP
                     NGTVSKDYTSGYKGSALGIMAHMNLHFATGAKIDPYWGIGIG"
     gene            complement(804..1415)
                     /locus_tag="VFU15_17510"
     CDS             complement(804..1415)
                     /locus_tag="VFU15_17510"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015363730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HEU4719648.1"
                     /translation="MKIVRPFKSLPPLNKKELNFDFVDYAWFTGYFTEDEVARIRTLW
                     SSDLADEAEVNNAGQALSRNDLRKSQVMFIRPDGQEWIYDKLAKACIQLNTKRFQFDI
                     RGFQTELQLANYSDQGFFDWHMDFGAGDISNRKLSITVQLSDPDEYEGGDLQFMINQN
                     IHTAPRAKGTAVIFPSFALHRVTPVTKGSRMSIVGWIAGTPYK"
     gene            complement(1457..2302)
                     /gene="accD"
                     /locus_tag="VFU15_17515"
     CDS             complement(1457..2302)
                     /gene="accD"
                     /locus_tag="VFU15_17515"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013927512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HEU4719649.1"
                     /translation="MSWFKRIKGGITTSTKEKKETPEGLWHKCPSCKKVFPTNEHAAN
                     YFVCQNCDYHDRINSEEYFAILFDEHQFTELDPDLTSGDPLHFEDTKKYPDRLADSQK
                     KTGLKDALRTAHGKINGRDLVVCCMDFSFIGGSMGSVVGEKISRAIDYCIANRYPLMI
                     ISKSGGARMMEAAHSLMQMAKTSAKLSLLADAHLPYISLLTDPTTGGVTASYAMLGDV
                     NIAEPGALIGFAGPRVVKETIKKDLPKGFQTSEFVLEHGFLDAIINRKDLKAKTAQLL
                     EFFMQ"
     gene            complement(2408..3466)
                     /locus_tag="VFU15_17520"
     CDS             complement(2408..3466)
                     /locus_tag="VFU15_17520"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014680580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HEU4719650.1"
                     /translation="MALSKIKELLGNQADSLLNHTCKTVSRDQLHLPGPDFVDRIFSI
                     SNRNPQVLRSLQAIYGHGRLADTGYVSILPVDQGIEHSAGASFAPNPIYFDGENIVRL
                     AIEGGCNAVATTFGVLGSVSRKYAHRIPFIVKINHNEFLSYPNRYDQNMFGSVEDAWE
                     LGAVAVGATIYFGSPESSRQIIEVSQAFERAHELGMATVLWCYLRNPGFKKDGVDYHA
                     SADLTGQANHLGVTIQADIIKQKLPVNNGGYNAVGHGKTSPKVYSELTTDHPIDLCRY
                     QVLNCYSGRMGLINSGGESKGASDLAEAVTTAVINKRAGGQGLISGRKAFQKPMKEGV
                     QLLNAIQDVYLEKEITIA"
     gene            complement(3601..>4123)
                     /locus_tag="VFU15_17525"
     CDS             complement(3601..>4123)
                     /locus_tag="VFU15_17525"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HEU4719651.1"
                     /translation="QAIGDHQQIVARIYAGVGVPGKNFPTLPLEKSFYGGGANGIRAW
                     ESRSLGPGSYSTPADQKYSQFGDIQIEYNVELRFKITKTLNGALFADGGNIWLLKYDS
                     ARAGANFTKNFYNDFAFGPGIGLRYDLGFFIIRLDWAAKIRDPSYDYGERWYVPGRRP
                     LSTNLNFGIGYPF"
BASE COUNT          874 a         1038 c         1179 g         1032 t
ORIGIN      
        1 taaccgatac caatgcccca gtagggatcg atcttggctc cggtggcaaa gtgaaggttc
       61 atgtgagcca tgatcccgag ggcagatcct ttgtagccgg acgtataatc cttgctgacc
      121 gttccgttcg ggttgcccag cgaatcatac gtcgtgatcg attcttcatc ggtccactgg
      181 actttgtagc tgaggtaacg gatgctcagg ccaaggctga gcttatcggt aacgccgtaa
      241 tcgaatttca cgtgcatggg cccgaagccg gataccttga atccgaggtt cgactcataa
      301 gctttaaaaa cggctttcgt gagattggga aatccgtagc cggccgtgat gacaaatttg
      361 ccctgcccga aggcctggtc gcgtcctctt cctcctcctt tgtgtttttt cctgcgcttg
      421 tgcttggcca ggtcatagtc gtcgtaatcg taagccagga aggctggccg gaaatccggt
      481 tttacggtgg tacctgcgtt cgttgcggat gcaggcctga atgcgctgcg gtctgcctgc
      541 tgtgcagaca gaatgcgtgt aatgcataat gccgtgatca gcattatgat cttccattga
      601 ggtttcataa agttgtaaat tagggttacg ccaaaagtag gaaatacgcg taagggaaag
      661 tgttaaaaat gcctctcatc gcggattgtt ttataaacgt gccacgttca taaaatcgcc
      721 ttttgaaatt tgcccgggga gtgttttcga aatgaaatat ctttttctcc gccccggcat
      781 gcaatgtgtt ttaccgcaat cgcttatttg tagggcgttc cggcgatcca gccgacgata
      841 gacatgcggc tgcctttcgt aacgggcgtt acgcggtgca gcgcgaaaga aggaaaaata
      901 acggccgtgc cttttgcgcg cggcgccgtg tggatattct ggttgatcat gaactggaga
      961 tcgccgcctt catattcgtc cggatcggaa agctgcaccg tgatgctgag cttgcggttg
     1021 gaaatgtcgc ctgcgccgaa atccatgtgc cagtcgaaaa aaccctggtc gctgtaattg
     1081 gccagctgca actcggtctg gaaaccacgg atgtcgaact ggaaacgttt cgtgttgagc
     1141 tgtatgcacg ctttcgcaag tttgtcgtag atccattcct gcccgtcggg gcggatgaac
     1201 atcacctgcg atttgcggag gtcattccgt gaaagcgcct gtcccgcgtt gttcacttcg
     1261 gcctcatccg cgaggtcgct gctccagagt gtgcggatcc gggctacttc gtcttccgtg
     1321 aaatacccgg tgaaccaggc gtagtcgaca aaatcgaagt tcagctcttt cttattgagc
     1381 gggggaagcg atttaaaggg ccggacgatc ttcatgattg tttatgaatg aggaaaacac
     1441 gcctggtgcg atggctttat tgcataaaga actccagcag ctgcgccgtt tttgctttga
     1501 ggtctttacg gttgatgatc gcgtccagga agccgtgctc aagaacgaac tcggatgtct
     1561 ggaatccttt cggaagatct tttttgatcg tttccttcac cacgcgcggt ccggcaaacc
     1621 cgataagcgc gccgggctct gcaatattga cgtcgccgag catggcgtaa gatgcggtga
     1681 cgccaccggt cgtcgggtcc gtcagcaggg aaatgtaggg caggtgcgca tccgcgagaa
     1741 gggaaagctt ggcggaggtt tttgccatct gcatgagcga atgggccgcc tccatcatcc
     1801 gtgccccgcc cgatttggaa atgatcatca gcgggtaacg gttggcaatg cagtaatcga
     1861 tggcgcgtga tattttttca cccaccacgg atcccatcga tccgccgatg aaggaaaaat
     1921 ccatgcagca tacgacgagg tcgcgcccgt tgatcttgcc atgcgcggtg cgcagcgcgt
     1981 ctttcagacc ggtcttcttc tgcgaatcgg caaggcggtc ggggtatttt ttcgtgtctt
     2041 cgaaatgcag cggatcgccg gacgtcaggt ccgggtccag ctcggtgaat tggtgctcat
     2101 caaaaagtat cgcgaaatat tcttcggaat tgatgcggtc gtggtaatcg cagttctggc
     2161 atacaaagta attggccgca tgctcgttgg tcggaaaaac ctttttgcag gacgggcatt
     2221 tgtgccagag accttccgga gtttctttct tctcctttgt tgatgtggtg atgcctcctt
     2281 ttatcctttt gaaccaactc attcgggatg tattgattta cgaattacga tttacgaagt
     2341 taactaaatt gaaaaggggc gtttatgcat atcgacggac tgtagtaatc ggaagaaggt
     2401 ctgccggtta cgcaatcgta atttcttttt ccaggtacac atcctgtatc gcgttcagca
     2461 gctggacacc ttccttcatc ggcttctgga atgctttccg gcccgagatc agtccctgtc
     2521 cgccggcgcg tttgttgatc acggcggtcg tcactgcttc ggcaagatcg gaagcgcctt
     2581 tcgattctcc gccggagttg atcaggccca tgcggcctga atagcagttg agcacctggt
     2641 agcggcaaag atcgatcggg tgatcggtgg tcagctcgga ataaactttc gggcttgttt
     2701 ttccgtgacc caccgcgttg tacccgccgt tgttcaccgg gagcttctgc ttgatgatgt
     2761 cggcctggat cgtgacgcca agatgattgg cctggcctgt gaggtcggcc gaagcgtggt
     2821 aatcgacgcc gtcttttttg aaccccggat tacgcaggta gcaccagaga acggttgcca
     2881 ttcccagctc gtgcgcgcgc tcgaacgcct gcgacacttc gatgatctgg cgggacgatt
     2941 cgggcgatcc gaaatagatg gtggcgccga cggccactgc gccgagctcc cacgcatctt
     3001 ccacggagcc gaacatgttc tggtcgtaac ggttcgggta ggaaagaaat tcgttgtgat
     3061 tgatcttgac gatgaacgga attctgtgcg cgtatttgcg cgaaacgctc ccgagcacgc
     3121 cgaacgtggt ggcaaccgca ttgcatccgc cttcgatcgc gagcctcaca atattttccc
     3181 cgtcgaaata gatcgggttg ggcgcaaaag aagcgcccgc ggaatgttcg atgccctggt
     3241 cgaccgggag aatggaaaca tacccggtat ccgccaggcg gccatgacca taaatggcct
     3301 gcaggctgcg cagcacctgc ggattacggt tgctgatgct gaaaatgcgg tcgacaaaat
     3361 caggacccgg caggtgaagc tggtcgcggg aaactgtttt gcaggtatgg ttcagcaaac
     3421 tatcagcttg attgccaaga agttctttga tttttgaaag ggccattcga atgctggttt
     3481 tttggacgtt tcagggcagc aaaagtaaca ttttttacta tgcacagaat ccttttcctc
     3541 cggtcgtcaa atacagttcc ggcctgggtt aacagattgt aaatcaaatg gtgaggagga
     3601 tcagaacggg tagccgatcc cgaagttgag attggtggaa agcggccggc ggcccggaac
     3661 gtaccatctt tcgccgtagt cataggaagg atcgcggatc ttcgcggccc agtcgaggcg
     3721 gatgatgaaa aagccgagat cgtagcgcag gccgatgccg ggtccgaacg cgaaatcgtt
     3781 gtagaaattt ttcgtgaagt tggcacccgc gcgggcggaa tcgtatttca gcagccagat
     3841 atttccgccg tcggcgaaca gcgcgccgtt gagcgttttc gtgattttga aacggagctc
     3901 gacgttgtat tcgatctgta tgtcgccgaa ctgcgaatat ttctggtcgg ccggcgtcga
     3961 ataggaacct ggcccgaggc tgcgcgactc ccaggcgcgg atgccgttcg cgccgccccc
     4021 gtaaaagctt ttttccagcg gaagcgtcgg gaaattcttt cccggaacac cgacaccggc
     4081 gtagattctt gcaacgatct gctggtgatc gccgatcgcc tgg
//
LOCUS       DASTSF010000624         9380 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_52757,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000624 DASTSF010000000
VERSION     DASTSF010000624.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 9380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9380
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52757"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1394
                     /locus_tag="VFU15_17530"
     CDS             <1..1394
                     /locus_tag="VFU15_17530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014201240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU4719652.1"
                     /translation="ISEDVSRVRMYIGPAVMYTTTTLITVIITISFMVSQDARLSLFV
                     LAPLPVLVISIYYVSNIINRKSLNVQQQLSLLSTYAQETFSGIRVVKAFTREDVSKER
                     FTSAAEEYKTRNMSLAKTEAMFQPFMMVLIGSSIILTVLIGGYETIEGKITPGTINAF
                     VLFVIRLTWPIASLGWVTSLVQRAAASQERINEFLKTKPAITNPTTEPLELEGKIEFR
                     NVTFTYPESGITAVDHISFTVEPGASLALIGKTGSGKSTIGKLLVRLEDVSSGEILID
                     GKDIRKINLGDLREKTGYVPQEVFLFSETVAGNIGFSLHDLRDLDKNREAIERAAKDA
                     AVYANIMEFPDKFETLVGERGVTLSGGQQQRISIARAILKHPDILIFDDCLSAVDTET
                     EDEILGNLRRIMKGKTTVIISHRVSSVRQADQIIVLDNGRIIEQGNHNALLDKKGVYW
                     ELYRKQIEELQEK"
     gene            complement(1441..3960)
                     /locus_tag="VFU15_17535"
     CDS             complement(1441..3960)
                     /locus_tag="VFU15_17535"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2341 domain-containing protein"
                     /protein_id="HEU4719653.1"
                     /translation="MKKNYLILSSLFIFCTLAGFAQPAGWSYSLPLTITENSGSLVTD
                     YQAKLIIDTQTPIGAGQMQTNGDDIRFGKDCAGNTLYNYWIESGINTTTTIIWVKIDT
                     LFASSTRTFYMFYGNNAATPVSAVNGTFFGPQSSTDSVASGSAGGVGNSQRGFRFSPN
                     EDILVTDFGKREPTGTTRYVTLFDFSTQAIVAQTQVSGPAAQYSYSGLANPVWLTTGT
                     QYVLELYQGASDGYYFGTSSQIGQALTYYDMRYCNSCTQNSFPTNTLSNYQYGYPDLW
                     YYTKNNVSPAPTVGTGTALSVNAGPDAAFCYGDSAMIGGTATGGNSPYVYAWSPATGI
                     SSPSSGTTYANPLSTTTYTLTVSDNTGCVSSDVVTVTVNPLPVVVATANQMSVCPGGQ
                     VILAASGASTYNWLPVNMTGATITDTPLSATTYSVIGTDVNGCTDTATQMVGVYTPPV
                     VLATGTYASICDNAITTLTLSASGASTYSWMPISASGDTVTDLPSMSTTYTVVGTDVN
                     SCTDTAYFSVTVMPSPVVAVTGSDSICAGSCTVLMATATGGTPSYSYSWAPSGNTTPT
                     DTECPTASSCYTVTVADANGCADTQTVCNYVFPAPSVVSTGPPAVCAGDSATLNATGV
                     NISSISWSPSTGLNTASGYTVVASPAATTTYTVVGTSSDGCSDTTTQLLTVNPLPSVS
                     YTSSVDTVCATDPAFALSGGSPAGGVYSGPGVTGSNFSPSSGNGSHTITYTYTDANSC
                     SASATHAIVVDPCTGIQSYQSTGGVTIFPNPFSTSFTISRETDENATALITDVNGKVV
                     KTVQLNSSSTQISTEDLATGVYMIHIISDKGEQVFKLVREN"
     gene            4068..4451
                     /locus_tag="VFU15_17540"
     CDS             4068..4451
                     /locus_tag="VFU15_17540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015361036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3276 family protein"
                     /protein_id="HEU4719654.1"
                     /translation="MNHEGNNEQRYNDQAEIFSKPVKAGKRTYFFDVKATRGNDYYLT
                     ITESKKRFGDDGRFHYEKHKLFLYKEDFDKFADGLLEAINFIRNAQGNGQANHVNHEM
                     PQDNHHEAQTNHVSSDVSFEDLGNH"
     gene            complement(4502..5365)
                     /locus_tag="VFU15_17545"
     CDS             complement(4502..5365)
                     /locus_tag="VFU15_17545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HEU4719655.1"
                     /translation="MAESVQLVECPRDAMQGLHTFIPTEKKISYINSLLKCGFHTIDF
                     GSFVSPKAIPQLRDTAEVLGKLDLSSSPSKLLAIIANVRGAEDALKFDEISYLGYPFS
                     ISETFQKRNTNATIEQSLGRVEEIQQLCVRHSRELVVYISMAFGNPYGDPWNADVAVE
                     WTKRLSSMGIKIIALADTIGVSNPENISYLFSTLIPEFPGITIGAHLHTTPGSWKEKM
                     EAAWNAGCRRFDSAVKGFGGCPMATDKLTGNMATENVLSFLDEKKVSTGIDRSQFVRS
                     VGEAGSVFPSE"
     gene            5457..5867
                     /locus_tag="VFU15_17550"
     CDS             5457..5867
                     /locus_tag="VFU15_17550"
                     /EC_number="1.11.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0006972 - hyperosmotic response [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0033194 - response to hydroperoxide
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEU4719656.1"
                     /translation="MKRNATAVWLGTGKEGTGKLTTQSQLLNNVSYSYKSRFEDGPGT
                     NPEELLAAAHAGCFSMKLAFVLGAAGFTPESIETKCENQLANGTIEFSHLICTAKVPG
                     ITPEKFRECAEDAKANCPVSKSLNTVITMDAKLG"
     gene            complement(5870..6907)
                     /locus_tag="VFU15_17555"
     CDS             complement(5870..6907)
                     /locus_tag="VFU15_17555"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013996811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HEU4719657.1"
                     /translation="MYKAIIRPVLFLVDPEKIHHFVFSVIKFSFRIPGIASLMRAIYQ
                     VRSPKLETTLFGIKFPNRVGLAAGFDKNAKLFDELGGLGFGFVEIGTVTPEPQPGNDK
                     PRMFRLKADSGLINRMGFNNEGAEAAAKRLRKRKTNIVIGGNIGKNKITPNGNAGDDY
                     EKCFLALFDVVDYFVVNVSSPNTPNLRALQDKAPLTEILLRLEKLNAQKPVRKPILLK
                     IAPDLTDSQLDDIIDIVKTTGIDGVVATNTTISRENLRTPSSVLEAIGAGGLSGKPLT
                     KRSLEVVNYLSEKSGKAFPIIGVGGIHTAEDAIAMLNAGASLVQVYTGFIYEGPGIAK
                     KINKALLQIQS"
     gene            complement(6980..7777)
                     /gene="cdaA"
                     /locus_tag="VFU15_17560"
     CDS             complement(6980..7777)
                     /gene="cdaA"
                     /locus_tag="VFU15_17560"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016775801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HEU4719658.1"
                     /translation="MNNLPVLLFLAFRWLDLLDIFLVAVLIYQLYRLVKGTAALNIFI
                     GIAFFYVFYIVVDLMRLELFSTILGEFINVGVIALLIVFQPELRRFFLFVGSYNVVTN
                     QKFRKALFSWKTPSKTTGTDVGAVVKACRNMSESKTGAIIVFTRGNDLSFYANTGDPV
                     DARISVRMLESIFFKNSPLHDGAVLITGNTIRAARCVLPVTENDDFPAHLGMRHRAAV
                     GITENTDAIAIVVSEQTGEISVSMEGEMQNNITPEQLRNILEKKLDN"
     gene            complement(7826..8674)
                     /gene="folP"
                     /locus_tag="VFU15_17565"
     CDS             complement(7826..8674)
                     /gene="folP"
                     /locus_tag="VFU15_17565"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEU4719659.1"
                     /translation="MSQDTHFRLESRGRTLSLDEPAVMGILNVTPDSFYDGGKYADPG
                     KALAHAEKMIAEGAAIIDIGAVSTRPGSADPGEEEELKRLMAVLPSLRKKFPDVFISV
                     DTFRSSVAGKAIAEGADIINDISGGTMDENMFPFIAKKNIPYVLMHIRGTPDNMQHSP
                     VYEDVVKEVKEFLVSRALQLKKAGASQLILDPGFGFGKTVEHNFTLLSKLEQLASLGF
                     PLLAGFSRKSMITKFSGTGKNEALNATTVLNTMALNKGARILRVHDVKEATEAIRITS
                     YLRGLC"
     gene            8673..9227
                     /locus_tag="VFU15_17570"
     CDS             8673..9227
                     /locus_tag="VFU15_17570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010517861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1599 domain-containing protein"
                     /protein_id="HEU4719660.1"
                     /translation="MISAIKKTSEQYDAIIANCRDIFARKMKDYGTSWRMFRISSITD
                     QVFIKAQRVRTIEETGTQKVNDDIRGEYIGMVNYSVIALIQMELENDNRTELPYEEAL
                     ALYDKHATAARSLMQNKNHDYGEAWRDMRLSSLTDLILVKLLRIKQIEDNSGNTEISE
                     GIDANYLDIINYAIFALIRISEGK"
BASE COUNT         2242 a         2474 c         2500 g         2164 t
ORIGIN      
        1 ggatcagcga agacgtcagc cgcgtgcgca tgtacatcgg tcctgcggtc atgtacacga
       61 caaccacgct catcacggtt atcattacta tttccttcat ggtgagccag gatgcgcggc
      121 tttctctttt cgtgctggcc ccgctccccg tgcttgtcat atcgatctat tacgtcagca
      181 acatcatcaa ccggaaaagc ctgaatgtac agcagcagct ttcgctcctt tccacgtatg
      241 cgcaggaaac attttccggc atccgtgttg tgaaggcgtt tacgcgcgag gacgtctcga
      301 aggaacgttt tacctcggcc gctgaagaat acaaaacgag gaacatgagc cttgcaaaaa
      361 cggaagcgat gttccagccg ttcatgatgg tgctcatcgg ctcgagcatc atactcactg
      421 ttcttatcgg cggatatgaa accatcgagg ggaaaataac gccgggcacg atcaatgcat
      481 tcgttctctt cgtgatccgt ctcacgtggc ccattgcttc gctgggctgg gtcacctcgc
      541 tcgtgcagcg tgcagccgct tcgcaggaac gcatcaacga attcctgaaa acaaaaccgg
      601 cgatcaccaa cccgacaaca gagccgcttg aacttgaagg aaagatcgaa ttcaggaacg
      661 tgacgttcac ttatcctgaa tcgggaatta cggctgtcga tcacatttcg ttcacggtag
      721 aaccgggcgc ctcgctggcc ctgatcggga aaaccggttc gggaaaatcg acgatcggga
      781 aactgcttgt ccgtctcgaa gatgtgtctt cgggagaaat tcttatcgac ggaaaggaca
      841 tacggaaaat aaacctgggc gatctccgcg aaaaaacggg atatgttccg caggaagttt
      901 ttcttttctc ggaaaccgtg gccggcaata tcggtttcag tctccacgac cttcgcgatc
      961 tcgacaagaa ccgggaagcg atcgagcgcg ccgccaaaga cgcagcggtg tatgccaaca
     1021 ttatggagtt cccggacaaa ttcgaaacgc ttgtcgggga acggggcgtt acactttcgg
     1081 gcggacagca gcagcgcatt tccatcgcgc gcgctatcct caagcatccc gacattctca
     1141 ttttcgacga ttgtctttcc gctgtcgaca cagagaccga agatgaaatt ctcggcaacc
     1201 tgcgccgcat catgaaagga aaaaccaccg ttattatcag tcaccgcgtt tcctccgtcc
     1261 gccaggccga ccagatcatc gttctcgata acggcaggat catcgaacag ggaaatcaca
     1321 atgcgctcct ggacaaaaaa ggcgtgtact gggaacttta ccgcaagcag atcgaagaac
     1381 tgcaggaaaa atagaaagtc cccgggagcc ggggactttc tggttctgaa aaggttttct
     1441 ttagttttcc cggacgagct tgaacacctg ttcgcccttg tccgaaataa tgtggatcat
     1501 atagacacct gtcgcaagat cttccgtgct gatctgcgtg gaagatgaat tcagctgcac
     1561 cgttttcacc actttcccgt tcacatccgt gatgagagcc gttgcattct cgtccgtctc
     1621 gcggctgatg gtgaaagaag tgctgaacgg gttggggaag atcgttacac cacccgttga
     1681 ctggtacgac tggattcccg tgcacggatc gacaacgatc gcatgcgttg cggaagcgct
     1741 gcagctgttc gcgtccgtgt aagtgtaggt gatcgtatgc gagccattac ccgaagacgg
     1801 gctgaagttc gatcccgtta cgcccgggcc ggaataaaca ccacctgcgg gagatccgcc
     1861 gctcagcgca aatgcaggat ccgttgcaca aaccgtatca accgaagatg tgtaggaaac
     1921 cgaaggcagc gggttcacag taagcagctg cgtggttgta tccgaacatc cgtcggagga
     1981 agtcccgaca accgtataag ttgtcgttgc cgcagggctt gcgaccactg tgtaaccgga
     2041 agccgtattg agcccggtgg acggtgacca cgagatggaa ctgatgttca caccggttgc
     2101 attcagcgta gcggaatcac cggcgcagac agcgggcggc ccggtggaga ccaccgaagg
     2161 agcagggaaa acgtaattgc atacggtctg tgtatccgca cagccgttcg catcggcaac
     2221 tgtcaccgta tagcagctgg aagctgtagg acattccgta tctgtcggtg tggtatttcc
     2281 ggacggcgcc caggaatacg aatacgaagg tgttcctccc gttgcagtgg ccatgagcac
     2341 tgtgcatgaa cctgcgcaaa tgctgtcgct tcctgttacc gcaacgaccg gcgaaggcat
     2401 tacagttact gagaaatacg cagtatccgt gcagctgttc acatccgttc ccacaactgt
     2461 atacgtcgtg gacatcgaag gaagatccgt aacagtgtcg ccgcttgcgc tgatgggcat
     2521 ccaggaatac gtcgacgctc cggaagcaga aagcgtaagc gtggtgatcg cattgtcgca
     2581 aatggaagcg taagttccgg tggcaagaac gacaggaggc gtatataccc cgaccatttg
     2641 tgtcgcggta tctgtgcatc cgtttacatc cgttccgata acggaatacg ttgttgcgga
     2701 aagcggcgtg tccgtgatgg ttgcgcctgt catgttcacc ggcagccagt tgtaagtgga
     2761 agcgccgctg gccgcaagga ttacctgtcc gcccgggcaa acgctcatct ggttagctgt
     2821 ggcgacaacc accggtaacg gatttaccgt cacggtcacc acatcgcttg aaacgcatcc
     2881 tgtattatca gaaacggtga gcgtataagt tgtagtggaa agcggatttg catacgtagt
     2941 ccctgatgaa ggagaagaaa ttcccgttgc cggcgaccat gcataaacgt aaggactgtt
     3001 ccctcctgtc gctgttccgc cgatcattgc agaatcaccg tagcagaaag ctgcatcagg
     3061 acctgcattt acggacaacg cggttccggt acccacggtg ggggccggtg aaacattgtt
     3121 cttggtataa taccacaaat ccgggtaacc gtattggtaa ttgctgagcg tattggtggg
     3181 gaacgaattc tgcgtacagc tgttacagta acgcatatca taatacgtca gcgcctgccc
     3241 gatctgcgaa gatgttccga aatagtaacc gtcgctcgca ccctggtaaa gttcgagcac
     3301 atattgtgtg cccgttgtca gccagacagg attagcgaga ccagaatagg aatattgagc
     3361 tgcaggcccg ctcacctgtg tctgcgctac aatggcctgc gtggagaaat cgaacagcgt
     3421 cacataacgc gtcgttccgg tgggttcgcg tttcccgaaa tcggtcacga gaatatcctc
     3481 gttcggcgaa aaacggaaac cgcgctggct gttcccgact ccacccgcgc tgccggaagc
     3541 cacgctgtca gtagaagact gcggtccgaa gaaagtgccg ttgactgcag aaacaggcgt
     3601 agccgcatta ttcccataga acatgtagaa ggtcctcgtg gacgaagcga acagggtatc
     3661 aatcttcacc catataattg ttgttgtggt gtttataccc gattcaatcc agtaattgta
     3721 gagggtattc ccggcgcagt ctttcccgaa acggatatca tcaccgttgg tctgcatctg
     3781 tcccgcccca ataggcgttt gcgtgtcgat gatcagtttt gcctggtaat ccgtaaccag
     3841 cgaaccggag ttctcagtta tggtgagagg caatgagtag ctccagcctg ccggttgtgc
     3901 gaatcctgct aaagtgcaga aaatgaacag gctggaaaga atcaggtagt ttttcttcat
     3961 gttgttacaa gattttgtcg gataggtttt atgtgataaa tatattagaa aattcagggc
     4021 taattaaggt tttttgctat ttttgcttaa caccactaca taaaagcatg aaccacgaag
     4081 gaaacaatga gcagcgttac aatgaccagg cggagatttt ctccaagccg gtcaaggcgg
     4141 gaaaaaggac ttatttcttt gacgttaagg caacgcgggg aaatgattat tacctgacca
     4201 tcacggaaag caagaaacgt ttcggcgatg acggccgctt ccactacgaa aaacacaaac
     4261 ttttccttta caaggaagac ttcgataaat tcgccgacgg gctcctggaa gcgatcaatt
     4321 ttatccgcaa cgcacagggc aacgggcagg ctaaccacgt taatcatgaa atgccccagg
     4381 ataaccacca cgaagcgcag accaatcacg tcagctcaga cgtaagcttc gaagacctcg
     4441 ggaatcatta actgaaaata cttccacgaa tgaaccggca gcaatgccgg tttttttttg
     4501 cctattccga agggaacacg gaaccggctt cccccaccga ccgcacgaac tgcgaacggt
     4561 cgatgcccgt gcttactttc ttttcatcga ggaagctcag cacattttcc gtggccatgt
     4621 ttcccgtcag cttgtcggtg gccatggggc agccgccgaa tcccttgaca gcgctgtcaa
     4681 acctccggca gccggcattc caggcggctt ccattttttc cttccaggag ccgggcgttg
     4741 tgtggaggtg cgcgccgatc gtgatgcccg ggaattcggg aataagcgta gaaaaaaggt
     4801 acgagatgtt ttcggggttc gaaacaccga tcgtatccgc aagcgcaatg atctttattc
     4861 ccatggagga aagccgtttc gtccattcta ccgcgacgtc cgcattccac ggatcgccat
     4921 aaggattccc gaatgccatg gaaatgtaaa cgacgagctc ccggctgtga cgcacgcaaa
     4981 gctgctggat ctcttccacc cttcccagcg attgttcgat cgtcgcgttt gtgttgcgtt
     5041 tctggaacgt ttcggaaatg gagaacgggt aaccgaggta gctgatctcg tcgaatttca
     5101 gcgcgtcttc tgcgccccgt acgttggcga tgatcgcaag aagcttcgac ggggaagaag
     5161 aaagatcaag tttcccgagt acttccgccg tgtcgcgcag ctggggaatt gctttcggag
     5221 aaacgaagct tccgaaatcg atcgtgtgaa acccgcattt cagcaggctg ttgatgtagc
     5281 tgatcttttt ttccgtcggg atgaacgtat gaagtccctg cattgcatcg cgcgggcatt
     5341 cgacaagctg taccgattct gccatgacgt aaagataaat acgacttatt gttttcaatg
     5401 cccggatgtc cggaaggaat atctatttta gcccttcacc gctcaaaaaa atcaatatga
     5461 aaagaaatgc aaccgccgtc tggctcggaa ccgggaaaga aggcaccggc aagctcacca
     5521 cgcagagcca attgctcaac aacgtatcct attcgtacaa atcgcgtttc gaggacggac
     5581 cgggaacgaa ccccgaagag cttctcgccg ccgcgcacgc cgggtgtttc tcgatgaagc
     5641 tggctttcgt gcttggtgca gcaggattca cgccggaatc gatcgaaacg aaatgcgaga
     5701 accagctggc gaacggcacg atcgaatttt cacatctcat ctgcacggcg aaagttccgg
     5761 gcatcacgcc tgagaaattc agggaatgtg cagaagatgc gaaagcgaac tgccccgtct
     5821 cgaaatcgct gaatacggtg atcacaatgg acgcgaagct ggggtgaact cagctttgta
     5881 tctgcagcag cgctttattg atcttcttcg caattcccgg tccttcgtaa atgaagccgg
     5941 tgtatacctg tacgagactc gcgcccgcat tcagcatggc gatcgcatct tctgctgtgt
     6001 gaattccgcc cacgccgatg atcggaaatg ctttgccgga tttttcggaa agatagttca
     6061 ccacttccag cgaccttttc gtcaatggtt ttccgctgag tccgcctgcg ccgatggctt
     6121 cgagaacaga ggaaggcgtg cggagatttt cgcgtgaaat ggtcgtgttc gtggccacaa
     6181 caccgtcgat gccggttgtt tttacaatgt cgatgatgtc gtcgagctgt gaatcggtga
     6241 gatcaggcgc gatcttcagc aggatgggtt tccggaccgg cttctgcgca ttcagctttt
     6301 ccaggcgcag caggatctcc gtcagcggag ctttgtcctg cagcgcacga aggttcggcg
     6361 tattcggcga gctgacgttg acaacgaaat aatcgacaac gtcgaacagc gcgaggaaac
     6421 atttttcata atcgtcgccc gcgttcccgt tcggcgtgat tttgttcttg ccgatattcc
     6481 cgccgatgac gatgttcgtt ttccgtttgc gcagccgttt cgccgccgct tccgcgcctt
     6541 cgttgttgaa gcccatccgg ttgatgaggc cgctgtcggc tttcaggcgg aacatgcgcg
     6601 gcttgtcatt tcccggttga ggttccggtg tgacggtccc gatttccaca aagccgaaac
     6661 cgagtccgcc gagctcatcg aacagtttcg cattcttgtc aaatcctgcg gcaagcccga
     6721 cgcggttcgg aaattttatc ccgaaaagcg tggtttccag tttcggtgat cttacctggt
     6781 aaatggcgcg catgagcgac gcgatgccgg gaatgcggaa cgaaaactta atgacactga
     6841 aaacgaagtg gtgaattttt tccgggtcga caagaaacaa taccggccgg atgatcgcct
     6901 tgtacatggc tgcaaagata tttccaattt ccgattggcg gttgcccgct ggaaacctgc
     6961 caacgcgtcc ggcaaatttt taattgtcaa gcttcttctc caggatgttt cgcaactgct
     7021 ccggcgtgat gttgttctgc atctcccctt ccatggaaac ggagatctcg ccggtctgtt
     7081 cggaaacaac aatggcgatc gcgtccgtat tttccgtaat tccaaccgct gcgcggtgac
     7141 gcatgcccag gtgcgcgggg aaatcgtcgt tctccgtaac cggcagcacg caccgcgctg
     7201 ctcgtatcgt gttgcccgtg atgagcaccg cgccgtcgtg caggggactg ttcttgaaaa
     7261 aaatgctttc gagcatgcgt acggaaatgc gcgcatccac gggatcgccg gtgttcgcgt
     7321 aaaagctgag gtcgttcccg cgcgtgaaca cgatgatcgc cccggtcttc gattccgaca
     7381 tgttgcggca ggctttcact accgcaccga cgtccgtgcc cgttgttttc gaaggtgttt
     7441 tccacgaaaa aagcgctttg cggaatttct ggttggtgac gacattatac gagccgacga
     7501 acaggaagaa ccgcctcagt tccggctgga aaacaatgag caacgcgatc acgccgacat
     7561 tgatgaattc gccgagaatg gtggaaaaaa gttccagccg catgaggtcg acgacgatgt
     7621 agaaaacgta aaagaacgcg atgccgatga aaatgttcag cgccgctgtt cctttcacga
     7681 ggcggtacag ctggtagatc agcacggcga cgagaaaaat atccagcagg tcgagccagc
     7741 ggaacgcaag gaagagcagc acggggaggt tgttcatggc ggcaaattta tccattagcc
     7801 atggattacg cgggttttaa aaacatcagc acaggccgcg caggtaagac gtaatgcgga
     7861 tcgcttccgt tgcttctttc acatcgtgca cgcgcaggat ccgcgcgcct ttgttcagcg
     7921 ccatcgtgtt gagcacggtg gttgcgttca gcgcttcgtt cttgccggtg ccggaaaatt
     7981 tcgtgatcat ggacttgcgg gaaaatcccg ccagcaacgg aaaaccgagc gacgcgagct
     8041 gttccagctt cgaaagcagc gtgaaattgt gctccaccgt tttcccgaaa ccgaaaccgg
     8101 gatcaagaat gagctgcgag gcccccgctt ttttcagctg taacgcacgc gaaacgagga
     8161 actctttcac ctctttcacc acgtcttcat aaacgggtga atgctgcatg ttgtccggcg
     8221 tgccgcgtat gtgcatgagc acgtacggga tgtttttttt cgcgatgaac gggaacatgt
     8281 tttcatccat cgttccgccg gaaatatcgt tgatgatatc ggcaccttct gcgatcgctt
     8341 ttccggcaac cgaagaacgg aacgtgtcga ctgaaatgaa aacgtccggg aattttttcc
     8401 gcaatgaagg cagaacagcc atgagccgct tcagttcctc ttcctctccc gggtcggcgg
     8461 aaccgggccg cgtggaaacg gcgccgatat cgataatggc cgcaccttct gcgatcattt
     8521 tttccgcgtg cgcaagcgct ttcccgggat cggcatattt cccgccgtcg taaaacgaat
     8581 cgggtgtgac gttgaggatt cccatcaccg ccggttcgtc cagggaaagc gtgcgtcccc
     8641 ggctttccag ccgaaagtgc gtatcttggg acatgatttc tgccataaaa aaaacgtcgg
     8701 aacagtacga tgcaattata gccaactgcc gcgacatctt tgcgcggaaa atgaaggatt
     8761 acggcacgtc atggagaatg ttccgcattt cctccatcac cgaccaggtt tttatcaaag
     8821 cgcaacgcgt gcgcaccatc gaggaaaccg gcacgcagaa agtgaacgac gacatccgcg
     8881 gcgaatacat cggcatggtg aactattccg tcattgcgct gatccagatg gagctggaaa
     8941 acgacaaccg cacggagctt ccgtacgaag aagccttggc gctttatgac aagcacgcga
     9001 ccgcggcaag atctttgatg cagaacaaaa atcacgatta cggtgaagcc tggcgcgata
     9061 tgcggctcag cagcctcacc gacctgatcc ttgtaaaatt attacgcatc aaacagatcg
     9121 aagataattc cggcaacacc gagatctcgg agggcatcga cgcgaattac ctcgatataa
     9181 tcaattacgc tatctttgct ttgattcgca tttccgaagg aaaataagtt tatgggattc
     9241 ttcaaaagcc gtgttttcag agccatcgcg tccctcggcc ttgccgcatt gttctatttc
     9301 ctttgcccgc cctgcttcaa taaaatgtac ctgctcgggg tgcttgtcct gctggtgctg
     9361 atcatcaacc ttgaccgcgt
//
LOCUS       DASTSF010000625         5074 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1696967,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000625 DASTSF010000000
VERSION     DASTSF010000625.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5074
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1696967"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /locus_tag="VFU15_17575"
     CDS             <1..295
                     /locus_tag="VFU15_17575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719661.1"
                     /translation="VQAKEFMEAVKRLGNRSQVAVIIIPRNITTASAETRTYMARAER
                     ARYISRCAIVINSIAQRIIGNFFMRVNKPAMKTKLFNTPGQAWNWLCEAEEKE"
     gene            320..640
                     /locus_tag="VFU15_17580"
     CDS             320..640
                     /locus_tag="VFU15_17580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015480727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HEU4719662.1"
                     /translation="MQDDKQQNQQLNIELNEDVASGIYSNLAIISHTNAEFVVDFIQI
                     MPGVPKAKVKSRIILTPQHAKRLMKALRENVNRFESEFGSITDLEMPPNIFPMGFGGP
                     QGQA"
     gene            complement(715..2877)
                     /locus_tag="VFU15_17585"
     CDS             complement(715..2877)
                     /locus_tag="VFU15_17585"
                     /inference="COORDINATES: protein motif:HMM:NF017889.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007160 - cell-matrix adhesion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nidogen-like domain-containing protein"
                     /protein_id="HEU4719663.1"
                     /translation="MKHRILFLFSVFAFSAFAGAQSMSPQQWDQAKTSGQLDGKAPAA
                     LKTANDTTPVTYKLAPNNPNPQPATSSCACWQQRDNTWQIVPITSGSPPDYRNDDGST
                     AVINIPFNFCLYGTTWTQLYINNNGNVSFGSPYATYTASGFPVNGYAMVAPFWADVDT
                     RNALSGLPYYQVTPTHVIVQWDSVGYFNAYADKVNTFQLIITDGNDPIVPGGNVSFCY
                     KDMQWTTGDASGGTNGFGGSDAVVGANKGDGTNFIQFGEFNQPGGTYNGPATAGSGID
                     WLDNQSFIFDACTNSNNIPPTISGLSICDTFHLCVGDVLPLNTVFFSPEAGQITNITV
                     DTTGVPGFTIVTNSSGNTATLNSFFTAQTNNTGLNTIIITATDNGTPPATTVIPVFIE
                     VLQPPVTTVSNDTSICLGSPPIPIVATGGSGYTWSPSTGIACDTCGSTTASPTVTTSY
                     VVTIATPACDVHDTVVVTVDNVNANAGNDQTICSGQNVQLNATGGTTYSWSPSTGLNN
                     PNIANPVASPTITTTYTVTATSAIGCTGTDPVTINVSALPNPDFSFVPATVFVDSSFQ
                     FTDLSTGSVTSWMWHFGDGDTSTAQNPSHAYTQPGTYHVCLVTVSTNGCSDSVCSDVT
                     VMPFTVYPPNVFTPNGDGINDFLVFKNLEYYPGCMLEVYDRWGMKVYESNSYNNDWNG
                     KRMGTGADVVDGVYYYVLVGPGLDTPVTGFVHVMRGGK"
     gene            complement(2986..4317)
                     /gene="argH"
                     /locus_tag="VFU15_17590"
     CDS             complement(2986..4317)
                     /gene="argH"
                     /locus_tag="VFU15_17590"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003008317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEU4719664.1"
                     /translation="MKLWQKNSAPDKQVDLFTTGNDRELDLLMAKHDVIGSIAHAQML
                     SSCGLISAGEFHLLKKELKIILALIENGSFSIEDGIEDVHSQVEFMLTKKLGDTGKKI
                     HTARSRNDQSLLDIKLFIREEIQKLTGKTEELFPLLLSLAEQHSGVLLPGYTHFQVAM
                     PSSFGLWFSAYAESLCDDLELMLAAYRMADKNPLGSAAGYGSSFPIDREMTTTLLGFS
                     GMNVSSVYAQMTRGKTEKACATAIAALAFTLSKLAADCTLYMSQNFGFISFPEHLTTG
                     SSIMPHKKNPDVWELIRGKCNRLQSLPNELALLLANLPSGYHRDLQLTKEILFPAFHE
                     ISSCMDMTILMLENIRVHAPENEDDKYKYLFTVESVNELVKKGVSFRDAYVQTGMAVE
                     NGTYVRPASSVHTHTGSAGNLRTDLISAAFGEMKKRFSFGKAENAISALLC"
     gene            complement(4356..>5074)
                     /locus_tag="VFU15_17595"
     CDS             complement(4356..>5074)
                     /locus_tag="VFU15_17595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018478004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEU4719665.1"
                     /translation="AATAEEEISGKNGIELLFPHLGKIDAAIVGEPTQMKMAVAEKGL
                     LVLDCFAHGKSGHAARQEGDNAIRKAWKDVAWFMDFRFPEISPWLGSTQMNVTMISAG
                     TQHNVVPDKCSFTVDVRVTECYTPEDVLKTVRENVSCEVVPRSLRLRPSFIAEDHPLV
                     RAGKTSGLSAFGSPTLSDCALIPVPCIKIGPGDSVRSHTAGEFIFLTEIEHGIAAYVQ
                     LLENFGAAIGKKPTELSHHS"
BASE COUNT         1173 a         1269 c         1462 g         1170 t
ORIGIN      
        1 ggtgcaggcg aaagaattca tggaagcggt aaagaggctg gggaaccgca gccaggttgc
       61 tgttattatt attccgagaa acattacgac ggcaagcgca gaaacgcgca cgtacatggc
      121 aagagccgaa agagcgcggt acatttcgcg ttgcgccatt gtgatcaaca gcattgcgca
      181 gcgcatcatc gggaattttt tcatgcgcgt caacaagccg gcaatgaaga cgaagctgtt
      241 caacacgccg ggacaggcgt ggaactggct ctgcgaagcg gaagaaaaag aataacttta
      301 tgatccaata atttctatca tgcaggacga taaacagcag aaccagcagc tcaacatcga
      361 gctgaacgaa gacgtggctt cagggatcta ttccaatctc gccatcattt cacataccaa
      421 cgcggaattc gttgtcgatt tcatacagat catgcccggt gttccgaaag cgaaagtaaa
      481 atcgcggatc atcctcacgc cgcagcatgc aaaacgcctg atgaaggcgc tgcgtgaaaa
      541 cgtcaaccgt ttcgaaagcg aattcggatc gattaccgat cttgaaatgc ccccgaatat
      601 tttcccgatg ggattcggcg ggccgcaggg gcaggcgtaa cggaaattta ccggccggat
      661 acaaatgaaa aagggaagaa attttcttcc ctttttcatt ttccggatac gcgatcattt
      721 ccctccgcgc atcacgtgca caaagccggt gaccggcgtg tcgagaccgg ggccaacaag
      781 cacgtaatag taaacgccgt caacgacatc cgcaccggtt cccatgcgtt tcccgttcca
      841 gtcgttgttg tagctgttgc tttcgtatac tttcattccc cagcggtcat acacttcgag
      901 catgcagccg ggataatatt caagattttt gaaaacgagg aaatcgttta tgccatcgcc
      961 gttcggtgtg aagacgttcg gcggataaac cgtgaacggc atcactgtca catctgaaca
     1021 cacgctgtcg ctgcagccgt tggtggaaac cgtcacgaga caaacatggt acgtgccggg
     1081 ctgggtgtat gcatgtgaag gattctgcgc ggtcgatgtg tcgccgtcgc cgaaatgcca
     1141 catccaggac gtaacggaac cggtggaaag atccgtaaac tggaagctgc tgtcgacaaa
     1201 taccgttgcg ggaacgaatg aaaaatcagg attgggcagc gcggaaacat ttatcgtgac
     1261 cgggtccgtg cctgtgcagc cgatggcgct cgtcgcagtt actgtatacg tggtcgtgat
     1321 ggtcggtgaa gcaaccgggt tggcgatgtt cgggttgttc agacctgttg acggtgacca
     1381 tgaataagtt gttccgccgg ttgcgttcag ctggacgttc tggccggagc agatcgtctg
     1441 gtcgttgcct gcgttcgcgt taacgttatc gaccgtgacg actaccgtgt catgcacatc
     1501 gcacgccgga gttgcaatag tgacaacata cgacgtggta accgtcgggg aggccgtagt
     1561 gctgccgcat gtgtcgcatg caattcccgt tgaaggcgac caggtataac cgcttccgcc
     1621 cgtggcaacg atcgggatgg gcggacttcc caggcagatg gaagtgtcgt tcgaaaccgt
     1681 ggtaaccggt ggctgcagta cctcgataaa gacggggatc actgtcgtcg caggtggagt
     1741 tccattgtcc gttgcagtga taatgatcgt gttgagcccg gtgttattcg tctgtgcggt
     1801 gaaaaacgaa ttgagcgttg cggtattgcc actggaattg gtcacgatcg taaacccggg
     1861 aacgccggtg gtatcgaccg tgatgttcgt gatctggccg gcttcgggcg agaaaaaaac
     1921 agtattgagc ggcagaacgt ctcctacgca aagatggaat gtgtcgcaga tgctgagtcc
     1981 cgaaatggtc ggaggaatgt tgttgctgtt cgtgcatgca tcgaaaatga acgactggtt
     2041 atcgagccag tcgatgccgc ttcctgctgt ggcagggcca ttgtatgttc cgccgggctg
     2101 gttgaattcg ccgaactgga tgaagttcgt accatcgcct ttattggcgc cgaccaccgc
     2161 atccgacccg ccgaagccat ttgttccgcc ggaagcatcg ccggtggtcc actgcatatc
     2221 cttgtagcag aaggagacgt ttccacccgg aacgatcgga tcgttcccgt cggtaataat
     2281 aagctggaac gtgttgactt tatcggcgta agcgttgaag tagccgacac tatcccactg
     2341 cacgatcacg tgtgtaggag ttacctggta atacggaagt ccgctgagcg cattgcgggt
     2401 gtcgacatcg gcccagaaag gcgccaccat cgcgtacccg tttaccggga acccgctcgc
     2461 agtataggtt gcataaggcg aaccgaaaga aacgttcccg ttgttgttga tgtaaagctg
     2521 cgtccaggtt gtgccgtaaa gacagaagtt gaacgggata ttgatgactg ccgtggagcc
     2581 gtcgtcgtta cggtaatcgg gaggagagcc cgaagtgatc gggacgatct gccaggtgtt
     2641 gtcgcgttgc tgccagcatg cgcaggagga cgtggccggc tgcggattcg ggttgttggg
     2701 agcaagcttg taagtaacgg gcgttgtatc gttcgcggtt ttgagagcgg ccggcgcctt
     2761 tccgtccagc tgcccgcttg ttttcgcctg gtcccactgc tgcgggctca ttgactgtgc
     2821 gcctgcgaaa gcggaaaatg cgaaaacaga aaagaggaaa agtatgcgat gtttcatgct
     2881 gaagaaaagt gaaattggtt tgcgtgttat aaagataaaa aaacatggtc gtgtttttcc
     2941 atcccggggc cggaaatgtt gctccctgca aagcaaagat caatttcagc aaagcagcgc
     3001 cgaaatggca ttttccgctt ttccgaatga aaaacgcttt ttcatttctc cgaatgcggc
     3061 ggagataagg tctgtacgca ggtttcccgc gctcccggtg tgtgtatgga cagatgatgc
     3121 aggacgtacg taggttccgt tctcaacggc catcccggtt tgcacgtatg cgtcgcgaaa
     3181 ggaaacgcct tttttcacca gctcgttcac ggattcgacg gtgaaaaggt atttgtactt
     3241 gtcatcctca ttttccggcg catgcacgcg gatgttttcc agcataagaa tagtcatgtc
     3301 catgcaggaa gaaatttcat ggaaagcggg gaaaagaatt tctttcgtca gctgcagatc
     3361 gcggtggtaa cccgacggaa gattcgccag cagcagggcc agctcgttgg gcagcgactg
     3421 gagccggttg cattttccgc ggatgagctc ccacacatcg ggattttttt tgtgcggcat
     3481 gatgctgctg cccgttgtga gatgttcagg gaatgaaatg aacccgaaat tctggctcat
     3541 gtacagcgtg cagtcggccg ccagctttga aagcgtgaag gcaagcgcag cgatcgcggt
     3601 cgcgcaggct ttttccgttt tgccccgcgt catctgggcg taaacggaac tgacattcat
     3661 gcctgaaaac ccgagaagcg ttgtcgtcat ttcacggtca atggggaatg acgaaccgta
     3721 gcccgccgcc gagccgagcg gatttttatc agccatgcgg tacgctgcaa gcatgagctc
     3781 aagatcatcg caaagacttt ccgcgtaagc gctgaaccac aacccgaacg aggaaggcat
     3841 cgcgacctgg aaatgcgtgt agcctgggag aagcacgccg ctgtgctgtt cagcgagcga
     3901 aagcaggagc gggaacagct cttccgtttt gccggtcagc ttctggattt cttcgcggat
     3961 gaagagcttg atgtcgagca gggactggtc gttacgcgaa cgcgccgtat ggattttctt
     4021 cccggtgtcg ccgagctttt tcgtcagcat gaattccacc tgtgaatgca catcctcgat
     4081 cccgtcctca atggagaaac tcccgttttc gatcagcgcg agaatgatct tcagctcttt
     4141 tttcagcaga tggaattctc cggctgaaat aagtccgcag gaagaaagca tctgcgcgtg
     4201 ggcgatagag ccaatgacat cgtgcttcgc catgagcaga tccagctcgc ggtcgttgcc
     4261 ggtggtgaac aggtctacct gcttgtcggg ggcggaattt ttttgccaga gcttcatcgg
     4321 tggtgagatg tgagtgttga gtattgaaac gtgaatcagg aatggtggga gagctcggtt
     4381 ggctttttac ctatagctgc gccgaaattt tccagcaact gaacataagc cgcgataccg
     4441 tgttcgatct ccgtgagaaa aataaattcg ccggctgtgt gtgagcggac ggaatcgccg
     4501 ggaccgatct ttatgcaggg aaccgggata agcgcgcaat ccgaaagtgt gggagaaccg
     4561 aaagcgctga gcccggaagt ttttccggcg cgcacgagcg ggtgatcttc cgcgataaat
     4621 gatggccgca ggcgcagcga acggggaacc acctcgcagg aaacattttc acgcacagtt
     4681 ttcagcacat cttccggcgt gtagcattcg gtcacgcgga catcgacggt gaaggaacat
     4741 ttatcgggta caacattgtg ctgtgtgcct gcgctgatca tcgtcacatt catctgcgtg
     4801 ctgcccagcc agggagaaat ttcggggaag cggaaatcca tgaaccatgc gacatctttc
     4861 cacgctttcc ggatcgcgtt gtcgccttcc tgccgcgcgg catgcccgct tttcccgtgc
     4921 gcgaaacagt cgagcacgag caagcctttt tccgcaacgg ccattttcat ctgcgtcggc
     4981 tcgccgacaa tagcagcatc gatcttcccg agatgcggaa aaagcaattc gatcccgttc
     5041 ttccctgaaa tttcttcttc cgccgtcgcg gcaa
//
LOCUS       DASTSF010000626        12758 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_446706,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000626 DASTSF010000000
VERSION     DASTSF010000626.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 12758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12758
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_446706"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            43..1212
                     /locus_tag="VFU15_17600"
     CDS             43..1212
                     /locus_tag="VFU15_17600"
                     /inference="COORDINATES: protein motif:HMM:NF018662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1302 family protein"
                     /protein_id="HEU4719666.1"
                     /translation="MFRLTSAFLLLPFFLAAQDSSAVKKKMKDYVQAGGYLKDLQVVS
                     FFPGDSIQTYNYIHNRLNFHFSPAKHLTAAVEVRNRIYYGQQVYDTPGYGKLVSQDNG
                     YFNLSALVVNGRSFVATTMIDRGWIDWSEKKWEVRFGRQRINWGKTLVWNPNDLFNAF
                     NFTDFDYEEQPGSDALRADYYPNGMSAVEMAVRPGRKKNEAVAAGLWRFNKRGYDIQL
                     LGGLYKTDIAFGTGWAGNIGNAGFKGEATWFQPKDKFTDTSGVLSATMTADYSFSNTL
                     YLEAAVLFNNHPVKNAFLFIPGQAAGTAISAKNLMPSEWSFFAQASGSPTPLITLSFA
                     SMYGADPGFVFIMPSCSYSISDNWDIMLLGQSVFFSSGKKIRSFSNAFLRLKWSF"
     gene            1479..2360
                     /locus_tag="VFU15_17605"
     CDS             1479..2360
                     /locus_tag="VFU15_17605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719667.1"
                     /translation="MAQQKSIVIGCDNADHLSAVMNTIKGTPAFSPEIISATRISDLL
                     SIVRSVTADLVIFCFRNNQFALNDFDIFVKKPAVPVLCLTQKTETGNLRWNPGNIVFT
                     CPFEHVKNDDYLNARIHSIFLLSSSSAMPYRADASLADIAAYNTENNVHDLSRYVLEL
                     DQKTEALQKIKGRITDLFPQVDDATRRELMSIVNAIRLSANNSKLWEDFRLYFEQMNP
                     GFLLLLAEKHPELTQKDLKYCCYLKMNMSNDDIKKLLGINQESVRTHKYRLKKKMELS
                     PEQDLVTYLRSVGKQGR"
     gene            2665..3234
                     /locus_tag="VFU15_17610"
     CDS             2665..3234
                     /locus_tag="VFU15_17610"
                     /inference="COORDINATES: protein motif:HMM:NF025431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4255 domain-containing protein"
                     /protein_id="HEU4719668.1"
                     /translation="MISNALQFTRDALDQYLKNLFGLGESKVLLNKLIENNGDVPPGN
                     QNKVLITLINIEKETLKPFYQRNQKNDNGGYSNMSTSERYNLFILISANFDDYTEGLQ
                     FLNACMQFFQVNQAIDSNSYSGMPADVGRLEYDIEKITYHQMHGLWTSMGARYLPSVV
                     YKTRLLTIQEGESYSFTSPVTSTSNSATA"
     gene            3231..4067
                     /locus_tag="VFU15_17615"
     CDS             3231..4067
                     /locus_tag="VFU15_17615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719669.1"
                     /translation="MMQSSYTPVFCADVLHSYFSEGICTCLRFIPSAATQPVLNRFGF
                     RMQGKTNGFTLFTSSPGPLSSLLSYITRVTGETAFSFDIISRNDGFPVFTEMPADRLV
                     LLTFDSSNSSGTAPGNIVSLVTVFTDVGEASVTGTLTLNFSDILNYINGNSYGLFRIS
                     LTARSTQWQYYVVNRNAMQLDNPSVTEKGGMTLEGPQPATTPAGEQALLFTSGSTLLP
                     LSEIPVYRFDLVNTPSNGAAQKKSSPKVVFRGLPNPGASNTAANAAGSSSLVTSPMYV
                     YV"
     gene            4101..5672
                     /locus_tag="VFU15_17620"
     CDS             4101..5672
                     /locus_tag="VFU15_17620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008202231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath C-terminal domain-containing
                     protein"
                     /protein_id="HEU4719670.1"
                     /translation="MNLSNIQTPGVYINEVNAFPNSVVAVATAVPAFIGYTPQAMYEG
                     KSYTNVPVQISSFADFQAYFCLPTPPAPAAAPSQYSPQYYLVAQKAQPTTGSYLQING
                     TYYSILPDPNTIYYLYNSVMMFYQNGGGTAYIVSVGSYGAPSGKAGTLGEQIINPNVK
                     LADLQNGLAQLLNQPEPTMYICPEATLLSVADNGTLMQSMLLQNSNMQTAVSIFDIIG
                     GNAPDPLLWPQDIANFRANTGTNGLKFGTAYYPFVGTTIMEDGDLNYTNLFGGDVTQL
                     SAIINPGNADPNVAAIIANIQNTSSGLTVEQNDDALTNASQNYSLIMQEVLLNANILP
                     PSGAMAGVITNTDNQVGPWQAPANTSIVGVASLPIYLSDSQQAGLNVDAVSGKSVNAI
                     RSFNGLGILVWGARTLDGNSQDWRYLSVRRTMIMLEQSCKLAAQAYVFAANDKNTWEA
                     IKAMIGGFLTSIWKQGGLAGASAAEAFSVSCGLGVTMTADDILNGFLNVTVKVAVTHP
                     AEFIVITFQQQQQTS"
     gene            5728..6192
                     /locus_tag="VFU15_17625"
     CDS             5728..6192
                     /locus_tag="VFU15_17625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019600221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEU4719671.1"
                     /translation="MATDDGSTEGATWPMPKFRFEVDLGTEMTGVAFQEVSGMDVENQ
                     IIEYRKSNSPLFSTEKMPGITKYGNVTMKRGVFVNDNTFWNWHAQIVMNTITRRTVII
                     KLLDENGKVTMQWTLNNAWPTKITSTDLKSDGNEVAVDTLEIAHEQLIISNG"
     gene            6185..6652
                     /locus_tag="VFU15_17630"
     CDS             6185..6652
                     /locus_tag="VFU15_17630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008469164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEU4719672.1"
                     /translation="MADATYPVSFYFSLSFVGEDAAFQEVSGISKELGIEEIVCGGEN
                     RFKYRLPTVATSQNLVLKRAIIPVGSMLMYWCSSCIDQGLASSIQPHNVILSLLNSDG
                     IVCMMWTFYNAYPVKYSVSDLKSTDNSIVIESIELAYQYFDISPISTFVNLFT"
     gene            6659..6829
                     /locus_tag="VFU15_17635"
     CDS             6659..6829
                     /locus_tag="VFU15_17635"
                     /inference="COORDINATES: protein motif:HMM:NF039278.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5908 family protein"
                     /protein_id="HEU4719673.1"
                     /translation="MPIEIREITIRTDVTSGANRLSNGAREKELSTLRKHLLEECRRI
                     ITLSAKKAGTKR"
     gene            6843..7523
                     /locus_tag="VFU15_17640"
     CDS             6843..7523
                     /locus_tag="VFU15_17640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007096245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719674.1"
                     /translation="MGASLELMKLTGYTDEEFQTPLSGQPYTVMINPDSIKWSRSVEY
                     NEQQPPDTSSPSQKYKNTPSEKLSFDIVIDCTGIVDSTRTSMATEISSLETICYTYNG
                     KIHRPNFVKVQWGKDISFNGVLTSFDTSYTLFRPDGSPLRAKISLSFSQYISVSTVTK
                     IDAPSSPDLTHMVTVVQDVNLPQLCTKVWNDYSFYIQVARYNGLNKFRNLKSGTKLIF
                     PPIIQSTS"
     gene            7523..9319
                     /gene="vgrG"
                     /locus_tag="VFU15_17645"
     CDS             7523..9319
                     /gene="vgrG"
                     /locus_tag="VFU15_17645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HEU4719675.1"
                     /translation="MASSGIQSGGVATFSVSIGGSPVPDSYVIHSVHVERRVNRIGTA
                     RIVVLDGEPNTGDFPASSSSTFVPGSTISIEAGYDASNTTIFQGIITGQSIRIDELIG
                     SALEVECRDASVKMTVGRKCLTFSNQKDSDIITSIIGNYSGLSSSVTATPTQWPEQVQ
                     YYATDWDFILSRAEANGMIITTINNKVTVAAPDADTTSVLTIGYGSGLMEFNADLNAV
                     TQLGAASASTWDFQNQAVASGQATVSYSGPGNLSSKTLSEVVGLSSYQLQTSAPLQTS
                     DLTDWSKALLVKSSYAKIMGVAKFQGTQLVDIAKYITLQGLGDRFNGDYFISGVSHDI
                     SNGNWLTDVSLGLSPQWFTEEPDVVAPPASGLLPGARGLFSGTVKQMYNDPDTQYRIL
                     VEVPLFDQNGAGIWARLANFYSTNGAGVFFLPEVGDEVIIAFLNEDPRYPVILGSVYS
                     STKNKPFNGLDPVQENTTKAIVSKSGISVEFNDTDKIFTINTPNKNTMILSDKDQKIT
                     IQDQNSNSIVMSSDGITIKSNNNITIDAGQKLTLKGATGIDVQASGGDVQTSGINIKE
                     SAQSQFSAQGSMTAQVQGGTELTLKGAMVMIN"
     gene            9332..9631
                     /locus_tag="VFU15_17650"
     CDS             9332..9631
                     /locus_tag="VFU15_17650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016941224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HEU4719676.1"
                     /translation="MPPAARLTDFHECPMQTPGVPPIPHVGGPVTGPGEPTVLIAGLP
                     AARVGDMLVCVGPPDSIVKGSSSVMIGGVPAARMGDQTAHGGQIVLGALNVMIGG"
     gene            9624..10058
                     /locus_tag="VFU15_17655"
     CDS             9624..10058
                     /locus_tag="VFU15_17655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007096242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HEU4719677.1"
                     /translation="MDRQLSKQENNFLGAGWAFPVAFSNGNYQVDSSSYEKNINDSID
                     IILQTKNGERAFDPQFGSGLQQFFFKKMDETLKGEIQDAVEAALLLNEPRITVQSVAV
                     EYADIQDGLVEITIVYIYNQTNTRHNYVFPFHLNEGTNLIDR"
     gene            10058..>12758
                     /locus_tag="VFU15_17660"
     CDS             10058..>12758
                     /locus_tag="VFU15_17660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719678.1"
                     /translation="MKPIDPQRAAASLEQALVPSPTLVDGRTERDFLAFIADYASLIN
                     FYDNTNNVSGNWVPFVMKDPVILLAAISKTDDRKMYSLFTRSCNLLKKALVKEQQELN
                     TSPGQEHQELPAKQKTRLEIPDRFNDLFERLTKVFMRIERWTYYMHIGTEGYDLKKYV
                     KRETKETFSVLFWALLTLQETLSNTSAIEGIAPVDYFPFEKWDEKTWKENRGKVPYWE
                     TLDLPEDFGMTKGTEQEKHALEVLCLDALVKAAAKIFSFVRTIVHHSHTAYRKMAGET
                     GRYPDTVLLRAFVHLLAVEQEQLNGISGRHLEFYYKSILKQSALPATPDSVFICATTA
                     KNSVYVLPSGTVFNGGTDANSNPVLFASEEVTVLNPASVTAAHTLSHWSDTSGTYFIL
                     EDISAPSTLKTDTSGATAAWNTFGGSYNRTPSLGFALASPLLYLAEGTRSIVITLTFQ
                     GTVTLPMLADAEFSLSTQKAWLELSTGQYTTGITQPAGSGQTVFTLTITLNPAQPAIE
                     TFAANPEGFSAEWPMLMISFNSFSQLAQPPVLQSAGINVSVSGLSTFSLYNDSSLLST
                     KAPYQPFGPIPEANGNFIIGNSEMFSKPLTDFSFTISWDNIPTTDFGTYYSAYNSYIN
                     AYPSQFQVPAAATTPVSSSSAQNPATSINPPASASPTATTATVTKTKSQNWRQRFLSW
                     VKSLFTGSSSSSPAASSSASSLSSSPAEETTTTSLTQVKTETFQFGNDSFEVMFNLFQ
                     GTQWNPLTMTSWTGVQPSTASFLFGPPSAASSPVYSGSTFSYSSKTPFASGMIDPSIQ
                     NETLTYTDASTYGFLKMQLSYPPYGFGSSLYPAVVSWVAYQNAQKLAGTSTSATTTKN
                     TLLSTTSTTTATTTSTASDLLSSPEPPFAPKVSSLTATYSASVT"
BASE COUNT         3175 a         3640 c         3194 g         2749 t
ORIGIN      
        1 gacgggaaga tcatttccga tgtgcagcaa aatccctgag cgatgttccg cctcacatca
       61 gcattcctgc tccttccatt ttttcttgcc gcacaggatt cttccgcggt aaaaaaaaag
      121 atgaaggact acgtgcaggc gggcggttac ctgaaagacc tgcaggtggt ttcctttttt
      181 ccgggcgaca gcatccagac gtacaattac attcacaacc gcctcaattt tcatttttcg
      241 ccggcgaagc atctcaccgc agcagtcgaa gtgcgcaacc gcatttatta cgggcagcag
      301 gtgtacgaca cgccgggtta cgggaagctt gtttcgcagg acaacggtta tttcaacctt
      361 tccgccctgg tggtcaatgg acggtctttc gtggcaacga cgatgatcga ccgcggctgg
      421 atcgactggt cggaaaaaaa atgggaagtg cgtttcggac ggcagcgcat caactggggc
      481 aaaacgctgg tctggaaccc gaacgacctg ttcaacgcgt tcaactttac cgatttcgat
      541 tacgaagagc agccgggcag cgatgcattg cgcgcggatt attacccgaa cgggatgtcg
      601 gcggtggaaa tggcggtgcg cccgggccgg aaaaaaaatg aagcggtggc agcgggtctc
      661 tggcgattta ataagcgcgg ttatgatatc cagctcctcg gcggacttta caaaacagac
      721 atcgcattcg gaacaggctg ggccggcaat atcggcaacg ccggcttcaa aggcgaagcg
      781 acgtggttcc agcccaaaga caaattcacc gacacgagcg gtgtgctcag cgcaacgatg
      841 accgccgact attctttttc caataccctt tatctcgaag cggcggtgct gttcaacaat
      901 cacccggtga agaacgcgtt cctgttcatt cccggccagg cggcgggtac ggcgatctcc
      961 gcaaaaaacc tcatgccttc cgaatggagc tttttcgccc aggcatccgg cagtcccaca
     1021 ccgctgatca cgctcagctt tgcatcgatg tacggcgccg atcccgggtt cgttttcatc
     1081 atgccctcgt gcagctattc gatcagcgat aactgggaca tcatgctgct cgggcagagc
     1141 gtcttctttt cttccggtaa aaagatccgc tccttcagca acgcctttct ccgcctgaag
     1201 tggagcttct gaaacgcggg aatttccagc ccggtgcaga tggtaaaatc catatcccgg
     1261 ttccgtctct ctaaacgtaa agtcaatcta tattctgttt aaagtgttgg taaaaactgt
     1321 atttcttttt gaaatttttg gtttatatct caaatattat aatattagcg ccctcccccg
     1381 cagtttttgc tgaaccgttt ttgtcgccgg ccctgcgagg ggcgcggtgc gtggaaagct
     1441 gcggataaca aaatcagaca ggagttttga tccgcaaaat ggctcagcaa aaatccatag
     1501 taataggatg cgacaatgca gatcacttgt cggcggtgat gaatacgata aaaggaacac
     1561 cagccttttc ccccgagatc atttccgcca ctcgtatctc tgacctgctc agcattgtgc
     1621 gctccgtcac ggcggatctc gtcattttct gtttccgcaa caaccagttt gccctgaacg
     1681 atttcgacat cttcgtaaag aaacctgccg tcccggttct gtgcctcaca caaaaaacgg
     1741 agacaggtaa cctccgctgg aacccgggca acattgtttt cacctgccct ttcgaacacg
     1801 taaagaacga cgattacctg aacgcacgca tccattccat tttcctgctc agttcctctt
     1861 cagccatgcc ctaccgtgca gacgcatcgc tggccgatat cgccgcttat aataccgaga
     1921 acaacgtgca cgacctcagc cgctatgtcc tggagcttga tcagaaaacg gaagcgcttc
     1981 agaaaataaa aggccgcatc acggatcttt tcccgcaggt agacgacgcg acacggcgcg
     2041 aactcatgtc cattgtcaac gctatccgtc tttccgccaa caacagcaaa ctgtgggaag
     2101 atttccgtct ttatttcgag cagatgaatc ccgggtttct cctgctgctg gcggaaaaac
     2161 atcctgagct tacgcagaaa gacctcaagt actgctgcta cctgaaaatg aacatgagca
     2221 acgacgatat caagaaactg ctcggcatca accaggaaag cgtacgtaca cataaatacc
     2281 gcctgaagaa aaaaatggag ctttcccccg agcaggatct cgtgacgtac ctcaggtctg
     2341 tagggaaaca gggcaggtaa ggagattttc agttcccgtt tttcagtttt ctgctcccgg
     2401 cttccggttc ccggcgttcc atttatcatt ggcgtttcta cgggatgaag ccgggaattc
     2461 ctgccgcgga ccaagaactg ctacctgcct gatgttttca gtttcatcct gccggcttcg
     2521 tgcacattcc gtgcgcgtct acgttttgtc tacgccgatt tcacgcgtcg caattgctgt
     2581 atacgaatgg tatatgacaa aaaacttcct gttagcgcat gcagcatagt tttacaccgt
     2641 tcaattttct gcagctgaca gacgatgata agcaatgctc tccagtttac gcgcgatgcg
     2701 ctcgatcagt acctgaaaaa tcttttcggg ctgggggaaa gcaaagttct tctgaacaag
     2761 ctgatagaaa acaatggcga tgttccgccg ggaaaccaga acaaagtgct gatcacgctc
     2821 atcaacatcg agaaggaaac actgaaaccg ttttaccagc gtaaccagaa gaatgacaac
     2881 ggaggttatt cgaacatgag tacgtcggaa cgttataacc tgtttatcct catcagtgcc
     2941 aatttcgacg attatacaga aggtcttcaa ttcctgaacg cctgcatgca gtttttccag
     3001 gtcaaccagg ccatcgattc gaattcctat tccgggatgc cggccgatgt gggaaggctt
     3061 gaatacgaca tcgagaagat cacgtatcac cagatgcacg gattgtggac gtccatgggg
     3121 gcgcgttacc tgccgtccgt cgtttacaaa acaagacttc tcaccatcca ggaaggcgaa
     3181 tcgtacagct ttacttcacc ggtcacttcc acttccaatt ccgcgacggc atgatgcaga
     3241 gcagctatac accggttttc tgcgcggacg tgcttcactc ctatttcagc gaaggtatct
     3301 gcacgtgtct tcgcttcatt ccgtccgcag cgacacagcc ggtgctgaac aggttcggtt
     3361 tccgcatgca gggaaaaacg aacgggttca cgcttttcac ttcttcaccc ggaccgcttt
     3421 cttcgctgct cagctatata acgcgcgtca ccggcgagac tgcattttcc ttcgacatca
     3481 tcagccggaa cgacgggttc cctgttttca cggagatgcc tgccgaccgc ctcgttctgc
     3541 tgacatttga cagcagcaat tcttccggca ccgctccggg gaacatcgtt tccctcgtga
     3601 ctgttttcac ggatgtcggg gaagcatccg ttaccggtac gctcaccctg aattttagtg
     3661 atattcttaa ctatataaat ggaaattcat acggcctgtt cagaatttca ttaacggccc
     3721 gttccacgca atggcaatat tatgtcgtta acaggaacgc catgcagctg gataatcctt
     3781 ccgtcaccga aaaaggcggg atgacactgg aaggtccgca gccggcgacc acgcccgccg
     3841 gcgagcaggc tcttcttttc acgtcgggca gcacgctgct cccgttaagt gaaattcccg
     3901 tttaccgctt cgatctcgtg aataccccgt cgaacggagc cgcacagaaa aaaagcagtc
     3961 cgaaggtcgt cttccgcggc ctgccgaatc ctggtgcctc aaataccgcg gcaaacgcgg
     4021 ccggcagcag cagtcttgtc acttcaccaa tgtatgtata cgtctaatcc attcacaaca
     4081 aaacaaaaaa agtcatctct atgaatttat caaacatcca gacacccggt gtatatatca
     4141 acgaggtcaa tgcttttccg aactcggtgg tagcagtcgc tacggcggta cctgcattta
     4201 tcggctatac accgcaggcg atgtacgaag gaaaatcgta cacgaacgtg ccggtacaga
     4261 tctcgtcttt cgcggacttc caggcgtact tctgcctgcc cacaccgcct gccccggctg
     4321 ctgctccttc gcagtacagc ccgcagtatt accttgtggc gcagaaagcg caaccgacga
     4381 ccggcagtta cctgcagatc aacggcacgt attattcgat ccttccggat ccgaatacga
     4441 tctattacct gtacaacagc gtaatgatgt tctaccagaa cggcggaggt acggcgtaca
     4501 tcgtttccgt cggttcctac ggcgcacctt ccgggaaagc cggaacgctc ggtgagcaga
     4561 tcatcaaccc gaacgtcaag ctggctgatc tccagaacgg tctcgcgcag ctcctgaacc
     4621 agcccgagcc cacgatgtac atctgcccgg aagcaacgct gctgagcgta gcggacaacg
     4681 gcacgctcat gcagagcatg ctgcttcaga attccaacat gcagaccgca gtcagcattt
     4741 tcgacatcat cggcggcaat gcgcccgatc cgctcctgtg gccgcaggac atcgcgaatt
     4801 tccgcgccaa caccggaacg aacggtctca aattcggaac agcttattat ccgttcgtcg
     4861 gaacaacgat catggaggac ggcgacctga actacacgaa tcttttcggc ggcgacgtta
     4921 cgcagctttc ggcgatcatc aatcccggca acgcggatcc gaatgtggcg gcgatcatcg
     4981 cgaacatcca gaatacttcg agcggcctta ctgtcgagca gaacgacgat gcgctgacga
     5041 atgcgagcca gaactacagc cttatcatgc aggaagttct cctgaacgcg aacattcttc
     5101 ccccgagcgg agcgatggcc ggcgtgatca ccaacaccga caaccaggtc ggtccgtggc
     5161 aggcgcctgc gaacacatcc atcgtaggtg tagcatcgct gccgatctac ctttccgatt
     5221 cgcagcaggc aggactgaac gtcgacgcag tttccggaaa atcggtgaac gcgatccgca
     5281 gcttcaatgg cctgggcatt ctcgtgtggg gagcacgcac cctggacggc aatagccagg
     5341 actggcgtta tctctccgtg cgccgtacga tgatcatgct cgagcagtcg tgcaagctgg
     5401 ctgcgcaggc gtacgtattc gcagcgaacg acaagaacac atgggaagcg atcaaagcga
     5461 tgatcggcgg cttccttacg tcgatctgga aacagggagg acttgccgga gccagcgcag
     5521 cggaagcatt ttccgtatcg tgcggtctcg gcgttacgat gacagcggac gatatcctca
     5581 acggttttct gaacgtaacg gtgaaagtag cggtcacaca ccctgccgaa ttcatcgtga
     5641 tcacgttcca gcagcagcag cagaccagct aagtgaacgt ataaattcat aaatattaaa
     5701 atcaacaact actaaaaaca ataagttatg gcaacagacg acggcagcac agaaggtgca
     5761 acatggccaa tgcctaaatt ccggttcgaa gtggatcttg gaaccgagat gactggtgtc
     5821 gcattccagg aagtttccgg gatggacgtg gaaaaccaga tcatcgaata ccggaagagc
     5881 aacagcccgc ttttctcgac agaaaaaatg ccgggtatca ccaagtacgg caacgtcacg
     5941 atgaagcgtg gtgtgttcgt gaacgacaat acgttctgga actggcacgc gcagatcgtg
     6001 atgaacacga tcacacgccg cacggtgatc atcaagctgc tcgacgagaa cggaaaagtc
     6061 acgatgcagt ggacgctgaa caatgcgtgg ccgacgaaga tcacgagcac cgatctgaaa
     6121 tcggacggta atgaagttgc agtggatacg ctagagatcg cgcatgaaca gctgatcatc
     6181 tcgaatggct gatgcgacgt acccggtcag tttctacttc tcactctctt tcgttggcga
     6241 agacgccgca ttccaggaag tttccgggat ctcgaaagag ctgggcatag aagagatcgt
     6301 ctgcggcggg gagaaccggt tcaaataccg gctccccacc gtagcgacaa gtcagaacct
     6361 ggtactcaag cgtgcgatca taccggtagg ctcgatgctc atgtactggt gctcgagttg
     6421 catcgaccag ggacttgcca gcagcataca gccgcataac gtcatcctga gtcttctcaa
     6481 ttcggatgga atagtatgca tgatgtggac attctacaat gcctacccgg tgaagtattc
     6541 tgtttcggac ctgaagtcga ccgacaacag cattgtcatc gagtccatcg agctggccta
     6601 tcaatacttc gacatttcgc ccatctcgac attcgtcaac ctgtttacct gaaacaaaat
     6661 gccgatcgaa atacgcgaaa tcacaatacg aacagatgtt acctccggcg ccaaccggct
     6721 ttcgaacgga gcgcgcgaga aggagctgag cacattgcgg aaacatctcc tggaagaatg
     6781 caggcgcatc atcacgctca gcgcgaagaa agcaggcacc aaacgctaaa cgaaaccgaa
     6841 tcatgggagc gagcctcgaa ctaatgaagc tgaccggtta taccgacgag gagttccaga
     6901 cgcctttgtc gggccagccc tatacggtta tgatcaatcc cgactcgatc aaatggtcgc
     6961 gcagcgtgga atacaacgaa cagcagccgc cggacaccag ctctccttcg cagaaataca
     7021 aaaacacgcc gagcgaaaaa ctgagcttcg acatcgttat agactgcacg ggcatcgtcg
     7081 actccacgcg cacgagcatg gcgacggaaa tctcgtcgct ggaaaccatc tgttacacat
     7141 acaacgggaa gatacaccgt cccaacttcg tgaaggtgca gtggggaaag gacatttcct
     7201 tcaacggcgt gctcacttca ttcgacacgt cgtacacgct tttccgtccc gacgggagcc
     7261 cgctgcgcgc gaagatctcg ctttctttca gccaatacat ttccgtttct acggtgacga
     7321 agatcgacgc gccttcatca cccgatctca cgcacatggt gacggtcgtc caggacgtca
     7381 acctcccgca gctctgcacg aaagtgtgga atgattattc attctacata caggtggcga
     7441 gatacaacgg gctgaacaaa ttccgcaacc tgaagtcagg cacgaaactc attttcccac
     7501 cgatcattca atccacctcc tgatggcaag ctccggcata caaagcggcg gcgtcgcaac
     7561 attctcggtt tccatcgggg gcagcccggt tcccgacagc tacgtcattc attcggtgca
     7621 cgtggaacgg agagtgaacc gcataggaac ggcccgtatc gtcgttctcg acggagagcc
     7681 gaacaccggg gatttcccgg ccagttcttc ctccacattc gttcccggct ccaccatcag
     7741 catcgaagcc ggttacgatg cgagcaacac tacgatcttc caggggatca tcacgggcca
     7801 gtccatccgc atcgacgagc tgatcggttc ggcgctcgaa gtagaatgcc gcgatgcttc
     7861 cgtgaaaatg accgtcggcc ggaaatgcct cacgttctcc aaccagaagg acagcgatat
     7921 catcacttct attatcggga attattccgg gcttagttcc agcgttacag cgacgccgac
     7981 acaatggccc gagcaggtgc agtattatgc gacggactgg gacttcatcc tgtcgcgcgc
     8041 agaagcgaac ggcatgatca ttacgacgat caacaacaaa gtgactgttg cagcaccgga
     8101 tgcggatact acttccgtac tcacaattgg ttacggatcc gggctaatgg agttcaacgc
     8161 cgacctgaac gccgttacgc agctgggcgc agcaagtgcc agcacgtggg atttccagaa
     8221 ccaggcggta gcttcgggcc aggcgacagt aagctacagc ggcccgggca atctttcttc
     8281 aaaaacactt tctgaagtgg tgggactttc ctcctaccag ctgcagacat cggcgccgct
     8341 gcagacatcc gatctcaccg actggtcgaa agcgctgctc gtgaagagtt cgtatgcgaa
     8401 gatcatggga gttgcgaaat tccagggcac gcagctcgtc gatattgcga aatacatcac
     8461 gctgcaggga ctcggtgacc gcttcaacgg cgactatttt atttccggcg tttcgcatga
     8521 catctcgaac gggaactggc ttaccgacgt gagcctcggt ctttccccgc aatggttcac
     8581 cgaagagccg gacgtcgtgg cgccgcccgc atccggactt cttcccggcg cacgcggact
     8641 tttttccggg acggtgaagc agatgtacaa cgaccccgac acgcagtacc gcatcctcgt
     8701 ggaagttccg ctgttcgacc agaacggtgc gggaatatgg gcgaggctcg ccaattttta
     8761 ttccacgaac ggcgcaggtg tttttttcct tcctgaagtc ggtgatgaag tgatcatcgc
     8821 atttctcaat gaagatccgc gttacccggt gatactcggc agcgtttaca gcagcacgaa
     8881 gaacaaaccg ttcaacggtc tcgatcctgt ccaggaaaac acgacgaaag ccatcgtttc
     8941 gaaatcgggc atctcggtcg agttcaacga cacggataaa atcttcacca tcaacacgcc
     9001 gaacaagaat acgatgatcc tgagcgacaa ggatcagaag atcacgatac aggatcagaa
     9061 ctcaaacagc atcgtcatgt cgagcgacgg catcacgatc aaaagcaaca acaacatcac
     9121 catagacgca gggcagaagc tgacgctgaa aggtgcgacg ggcattgatg tgcaggcatc
     9181 gggaggagac gtgcagacga gcgggatcaa catcaaggaa agcgcgcaga gccagttctc
     9241 cgcacaggga agcatgaccg cgcaggtgca gggcggaacg gagctgacgc tgaaaggtgc
     9301 gatggtgatg attaattaat aattgaaaag tatgcctcca gcagcaagac tgaccgattt
     9361 ccacgaatgc ccgatgcaga cgccgggtgt tcctcctatc ccgcacgtgg gcgggcccgt
     9421 gacaggtccg ggtgaaccga ccgttctcat cgccgggctt cccgccgcgc gcgtgggcga
     9481 catgctcgtg tgcgtgggtc cgcccgattc cattgtgaaa ggcagttcga gtgtcatgat
     9541 cggtggagtg ccggctgcgc gtatggggga tcaaaccgct cacggcggac agattgtcct
     9601 cggagcactt aatgtcatga tcggtggata gacaactcag caaacaggaa aataattttc
     9661 tcggcgcagg ctgggcattt ccggttgcct tttcgaacgg gaattaccag gtcgattcat
     9721 cgtcgtatga gaaaaacatc aacgactcga tcgacatcat cctccagacg aagaacggcg
     9781 aacgcgcctt cgatccgcaa ttcggatcag gactgcagca attcttcttc aagaaaatgg
     9841 atgaaacgct caagggagaa atacaggatg cggtagaggc ggcgctcctg ctcaacgaac
     9901 cccgcatcac cgtccaaagc gtcgccgtcg agtacgccga tatccaggac gggctcgtgg
     9961 aaatcaccat cgtttatatc tacaaccaga ccaacacgcg acacaattat gtcttcccgt
    10021 tccacctgaa cgaaggcacc aacctgattg accgctgatg aagcctattg atccgcaaag
    10081 agcagccgct tcgcttgaac aggcgcttgt tccttccccg acgcttgtcg acgggagaac
    10141 ggaacgggac tttcttgcgt tcatcgcgga ctacgcttcg ctgatcaatt tttacgacaa
    10201 caccaacaac gtttccggca actgggttcc tttcgtcatg aaagatcccg tgatacttct
    10261 tgcggcgatc tcgaagacgg acgaccggaa aatgtattcc ctcttcaccc gttcgtgcaa
    10321 cctgctgaaa aaagcgctcg tcaaagaaca gcaggagctc aatacatcac cgggacaaga
    10381 acaccaagag ctcccggcaa aacaaaaaac caggctggag atacccgacc gtttcaacga
    10441 tctcttcgaa cgcctgacaa aagttttcat gcgtatcgag cgatggacat attatatgca
    10501 tatcggtacg gaaggatacg acctgaaaaa atatgtgaag cgggaaacga aagaaacgtt
    10561 cagcgtactg ttctgggccc ttcttacgct gcaggaaaca ctttcgaaca cttctgctat
    10621 cgaaggcata gcgcccgtgg attattttcc cttcgaaaag tgggatgaaa agacctggaa
    10681 ggagaaccgc gggaaagttc catactggga aacgctggac ctcccggaag atttcgggat
    10741 gaccaaaggc accgaacagg aaaaacacgc gctcgaagtt ctttgcctgg atgcgttggt
    10801 gaaagccgct gcgaaaattt tctcctttgt ccgcacgatc gtccatcatt cgcataccgc
    10861 ctacaggaag atggcgggcg aaaccgggcg ttatcccgac accgtgcttc tccgcgcgtt
    10921 cgttcatctt ctcgccgtgg agcaggaaca actgaatggg atctccggaa gacatcttga
    10981 attttattac aaatccatcc tgaagcagtc ggcgcttccg gccacacccg acagcgtttt
    11041 catctgtgcg acgacagcga aaaattccgt ttacgttctc ccatcgggaa cagttttcaa
    11101 cgggggcacg gatgcaaaca gcaacccggt ccttttcgca tcagaggaag tcacggtgct
    11161 gaacccggct tccgttacgg cggcacacac gctatcgcac tggtcggata cttccggcac
    11221 ttattttatc cttgaagata tctccgcgcc gtccacgtta aaaaccgata caagcggcgc
    11281 gacagcagct tggaatacat tcggcggaag ctacaaccgc actccctccc tcggctttgc
    11341 gcttgcatcg cccctgcttt acctcgctga aggaacaaga agcatcgtga tcacgctcac
    11401 gttccaggga acggtgacgc tgcccatgct ggcagatgcg gaattcagtc tcagcacgca
    11461 gaaagcatgg cttgaactga gcacgggcca gtataccacc gggatcacgc aaccggcagg
    11521 ttccggccag actgttttca cgcttacgat cacgctcaat ccggcacaac cggccatcga
    11581 aacatttgcg gcgaacccgg aaggtttcag cgcagaatgg ccgatgctga tgatcagctt
    11641 caattcattt tcacaactgg cgcagccgcc ggttctccag tcagccggga tcaacgtcag
    11701 cgtatccggg ctgagcacgt tcagccttta caacgacagc agcctgctca gcacgaaagc
    11761 gccttaccag cctttcggtc ccatccctga agcgaacggg aatttcatta tcgggaacag
    11821 cgaaatgttc agcaagccgc tgacggattt ttcgttcact atcagctggg acaatatccc
    11881 cacgacggat ttcggaacct attacagcgc ctacaacagc tacatcaacg cctatccttc
    11941 ccagttccag gttccggccg cagcaacgac accggtcagc agcagcagcg cacagaatcc
    12001 ggcgacatcc atcaacccgc cggcttctgc gtcccccact gcgaccactg cgaccgttac
    12061 gaaaacaaaa tcgcagaact ggcggcaacg gtttttgtcc tgggtcaagt ccctgttcac
    12121 cggctcttca tcttcgtcgc ctgctgcatc ttcatccgct tcttcattat cctcttctcc
    12181 ggcggaggaa acgaccacaa cttctttgac gcaggtgaaa acggaaacgt tccagttcgg
    12241 aaatgattca ttcgaagtca tgttcaacct tttccaggga acgcaatgga atccgctcac
    12301 gatgacatcc tggaccgggg ttcagccatc caccgccagc ttcctgttcg gaccaccctc
    12361 cgctgcgtcg tctcccgtgt actccggcag cacgttcagt tattcctcga aaacaccgtt
    12421 cgcatccggc atgatcgatc cgtcgataca gaacgaaacg ctaacataca ccgacgccag
    12481 cacatacggt ttcctcaaaa tgcagttaag ctatccacct tatggcttcg gttcttcgct
    12541 ctatcccgct gtcgtttcct gggtcgctta ccagaacgcg cagaaactgg ccggcacaag
    12601 cacgagcgca acgacaacca aaaacacgct gctaagcacc acgtccacga ccacggcaac
    12661 cacgacttcc acggcgtcgg atcttctttc atcgccggag cctccgttcg cgcccaaagt
    12721 ttcatccctt acagccacgt attccgcgag cgttacgt
//
LOCUS       DASTSF010000627         4446 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_944862,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000627 DASTSF010000000
VERSION     DASTSF010000627.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4446
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_944862"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1506
                     /locus_tag="VFU15_17665"
     CDS             <1..1506
                     /locus_tag="VFU15_17665"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEU4719679.1"
                     /translation="LTDVEGPAVDYTNIAYTDNNDMVFVPNDTMRITGTFINYLGTIN
                     NLNTTLTVISGGSYVTILDGSHSVGTLGTMGTATQASDPYQIKINTNCPYNQTIVFKL
                     NMTDGVYSVNKYFSQIVNGDYINITINDIWTTIPSQGRIGFRNSQQSQGLGFCYQGSG
                     TLLYDASLMVGTSSAKVSDMIRGATAGQDDNDFQSQQIVQAITPPVVSDFDAMGVFRD
                     NISPAPLPVTVRHREFAWATAPHRKYIIVQYSIMNTGATTLSNMYAGIFADWDIDAST
                     SGQNRADVDNTNRMGYVYSTVAGGKYCGIKLLTNGAFNNYAIDNISGGGGGVNIYDNF
                     TTAEKYQTLSTSRAQAGTTGQGNDVCHVVSSGPFTVAAGDSIVVAFALIAGDDLSDLQ
                     NSAVDAQSMYNNLPLGSPVTVAHITTGLNAFPNPASGMTQISYNVAEGGNTDIRLVDA
                     QGRLVRMLDTGDKSPGAYSVMLDAAELSDGIYFVQMISNGKLTTQKFVVSH"
     gene            1615..2163
                     /locus_tag="VFU15_17670"
     CDS             1615..2163
                     /locus_tag="VFU15_17670"
                     /inference="COORDINATES: protein motif:HMM:NF044724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5606 domain-containing protein"
                     /protein_id="HEU4719680.1"
                     /translation="MDISKVISISGMPGLYKVIAQSKNGVIVESLTDQKRFPAFGSNR
                     ISTLEDISIYTTAEELPLKEVLRKIFDKEKGGNCIDPRSADEAAMREYMLSVVPEYDA
                     ERVHVSDLKKLFTWYNLLNASGLLTAKEEEKEGEEGEKKNLVPGTNKNIPSAKKEVGQ
                     KKNLKSNAPKVKAQGVRKTGTA"
     gene            2173..3897
                     /locus_tag="VFU15_17675"
     CDS             2173..3897
                     /locus_tag="VFU15_17675"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015029099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HEU4719681.1"
                     /translation="MEPVAGVETQPVRTFLPADLSFSSWKELEPFFRDLAERSITDVA
                     SLMTWLKDASELDSVFDEELAWRYIRSTCDTANAQYEEAYEFFISEIEPNAAPWFHAI
                     NRKLDECPFRSELDPGIFSVYLRSVHNDVAIFREKNIPLFTAIDQLKQQYGKIAGDMT
                     VTIDGKELTIQQASVFLKDPERKKREEAFLKIWERRLADKEKLDELFDQLTALRQQVA
                     ANADFENFRDYSFASLGRFDYTKEDCFRFHEAIEKEIVPFLASIEEKRKQKLGVDTLR
                     PWDMDVDVSGLAPLKPFRGGNELLDKTMRCLSAVRPAYSAFISTMRQKKHFDLDSRKG
                     KAPGGYNYSLAETGVPFIFMNSAGTQTDVVTMLHEAGHAIHSFLEHPLPLNALKSPPS
                     EVCELASMSMELISMEHWDAFYPDAAELKRARREQLEKILSTLPWVASVDAFQHWIYE
                     HKGHTPEERGRAWEKIMLRFGSPVIDWSGHQNIRFNNWQKQLHIFEVPFYYIEYGMAQ
                     LGAIAVWRNYLENPARALDAYESALKLGYTKPIGKLYEAAGIRFDFSREYVRELVTFV
                     REQLEKIN"
     gene            3905..4429
                     /locus_tag="VFU15_17680"
     CDS             3905..4429
                     /locus_tag="VFU15_17680"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEU4719682.1"
                     /translation="MITPPPSTDYAAYYETYISLIRGRDPLKMLESQVFDFKALLSEI
                     PAEKEEFRYAEGKWSVKEVIGHINDTERVMSYRALCFARGEKKELPGFEENDYAKAGM
                     FDKRSLYDLGHEFGFIREATIALFKSMSADVLDNRGIANHNTVTPRALLYIIAGHHQH
                     HERVLRERYLADIL"
BASE COUNT         1139 a         1176 c         1189 g          942 t
ORIGIN      
        1 cttaccgacg tagaaggacc tgcagttgat tataccaaca ttgcctatac ggataacaac
       61 gacatggttt tcgttcccaa tgacacgatg cgaatcacag gaacgttcat caactacctc
      121 ggaacgatca acaacctcaa cacgacgctt accgttattt ccggcggatc ttacgtgacc
      181 atcctcgacg gctcgcacag tgtgggaacg ctcggcacga tgggaacggc cacgcaggca
      241 agcgatccct accagatcaa gatcaacacg aattgcccgt ataaccagac gatcgtgttc
      301 aagctgaaca tgacggacgg cgtttacagc gtgaacaagt atttttcgca gatcgtcaat
      361 ggcgattaca tcaacatcac gatcaatgac atctggacga cgatccccag ccagggccgc
      421 atcggtttcc gcaacagcca gcaatcgcag ggcctcggtt tctgctacca gggcagcgga
      481 acgctcctgt atgacgcttc gctcatggtg ggaacttctt ccgcgaaagt cagcgacatg
      541 atccgcggcg caacagccgg gcaggacgac aatgatttcc agtcgcagca aatcgtacag
      601 gcgatcacgc ctcccgttgt ttccgatttc gatgcaatgg gtgttttccg cgataatatt
      661 tccccggcgc ccctcccggt aaccgtaagg cacagggaat ttgcatgggc cactgcgccg
      721 cacaggaaat atatcatcgt ccagtacagc attatgaata cgggcgccac aacgttgtcc
      781 aacatgtacg ccggaatttt tgccgactgg gacatcgatg cttcgacgtc aggacagaac
      841 cgcgcggatg tggataacac gaaccgcatg ggttatgttt attcaactgt cgcgggcggt
      901 aaatactgcg gcatcaagct gctcacgaac ggcgccttca acaactatgc gatcgacaac
      961 atcagcggcg gcggcggcgg tgtgaatatc tacgacaatt tcacgaccgc agaaaaatac
     1021 cagacacttt ccaccagccg cgcccaggcg ggcacaacag gccagggcaa cgacgtgtgc
     1081 cacgtggtga gctcaggtcc gttcactgtc gcagcaggag attccatagt ggtggcattt
     1141 gcgctcatcg ccggcgatga cctcagcgac ctgcagaaca gcgcggtcga tgcgcagagc
     1201 atgtacaaca atcttccgct cggctcaccg gtaacggttg cgcacatcac cacgggactc
     1261 aatgcattcc ccaacccggc ttccggaatg acacagatca gctacaacgt ggcagaaggc
     1321 ggcaacacgg acattcgtct cgttgacgcg cagggacgtc ttgtacgcat gctcgatacc
     1381 ggggacaaat cacccggggc gtattcggtc atgctggacg cagctgaact ttccgacggc
     1441 atctacttcg tacagatgat ctccaatgga aagctgacca cgcagaaatt cgtggtttcg
     1501 cactgacagc attgaaaaaa tgaacaggac ggcggtcgtg aaggccgccg tttttttatt
     1561 tcccggcctc ttcccgtcgt ccttttatta tttttgtgtg aaatacaaag caatatggat
     1621 atcagcaaag tgatctcgat ctccggcatg cccgggctct ataaagtgat cgcacaatca
     1681 aagaacggcg tgatcgtaga gtcactgacc gatcagaagc gttttcctgc attcggaagc
     1741 aaccggatca gcacgctgga agacatcagc atctacacca cggccgagga gcttccgctg
     1801 aaggaagtcc tccggaaaat attcgacaaa gaaaaaggcg gcaactgcat cgatccccgt
     1861 tcggcggacg aagcggccat gcgcgaatac atgctttctg ttgttcccga atacgacgcg
     1921 gaacgcgtgc acgtttccga tctgaaaaag ctgttcacct ggtacaacct gcttaatgca
     1981 tccgggcttc tcacggcaaa agaagaggaa aaggaaggcg aagaaggtga aaagaaaaat
     2041 ctcgtgccgg gaacgaacaa gaacattccc tccgccaaga aggaagtggg tcaaaagaaa
     2101 aacctgaagt cgaacgcacc caaggtaaaa gcgcagggtg ttcgtaaaac gggcacggcc
     2161 tgacctggga tcatggaacc cgttgccggt gtcgaaacgc agcctgtaag aacttttctt
     2221 cccgctgatc tttctttttc ttcgtggaaa gagctcgaac cttttttccg cgatcttgcc
     2281 gaacgcagca ttacggatgt tgcttcgctg atgacctggc tgaaagacgc cagcgagctg
     2341 gattccgttt tcgacgaaga gctggcctgg cgttatatac gttccacctg cgacacggcg
     2401 aatgcgcaat acgaggaagc gtatgagttt ttcatttccg aaatagagcc gaatgcggcg
     2461 ccgtggtttc atgcgatcaa ccggaagctg gatgaatgcc cgttccgcag cgagctcgat
     2521 cccggcatat tttccgttta cctcaggagc gttcataacg atgtggcgat cttccgcgaa
     2581 aaaaatatcc cgctgttcac cgcgatcgat caactgaagc agcagtacgg gaaaattgcg
     2641 ggcgacatga ccgtcaccat tgacgggaaa gagctgacga tccagcaggc atctgttttc
     2701 ctgaaagacc ctgaacggaa aaaacgcgaa gaagcatttc tcaaaatatg ggagcgcagg
     2761 ctcgccgata aggaaaagct cgatgagctt ttcgatcagc tcactgccct ccggcagcag
     2821 gtggcggcaa atgcggactt tgagaatttc cgcgattaca gtttcgcctc gctcggaaga
     2881 ttcgattaca cgaaagagga ttgtttccgt tttcacgaag cgatcgaaaa agaaatagtt
     2941 cctttcctcg cctcgatcga ggaaaaaaga aagcagaagc ttggcgtgga cacgctgcgt
     3001 ccgtgggaca tggatgtcga cgtcagcggg ctggcgccgc tcaaaccttt ccggggcggg
     3061 aacgagctcc tcgacaaaac gatgcgctgc ctcagcgcgg tgcggccggc ttacagcgct
     3121 ttcatttcga cgatgcggca gaaaaagcat ttcgacctcg attcacggaa aggaaaagcg
     3181 cccggcgggt ataattattc actggcggaa acaggcgttc cgttcatttt catgaattcg
     3241 gccggtacgc aaacggatgt ggtgacgatg cttcatgaag caggacacgc gatccattct
     3301 ttcctcgaac atcctcttcc gctcaacgca ttgaaaagtc cgccgtcgga agtgtgcgag
     3361 ctggccagca tgtcgatgga gcttatttcg atggagcact gggacgcatt ttatcccgat
     3421 gcagcagaac tgaaacgcgc acggcgcgag cagcttgaaa agatactttc caccctgccg
     3481 tgggtggcat ccgtcgatgc attccagcat tggatatacg agcacaaagg ccacacgccg
     3541 gaagagcgcg gccgcgcatg ggaaaagatc atgctgcgtt tcggctcgcc ggtgatcgac
     3601 tggagtggcc atcaaaacat acgcttcaac aactggcaga aacagctgca tatttttgaa
     3661 gttccttttt attacatcga atacgggatg gcccagctgg gtgcgatcgc ggtgtggagg
     3721 aactatcttg agaatcctgc gcgcgcactc gatgcgtatg aaagcgcgct taagctgggc
     3781 tatacgaaac cgattggaaa gctgtacgaa gcggccggga tacgtttcga tttttcgcgc
     3841 gagtatgtgc gtgaactcgt aacgttcgtc agggagcagc ttgaaaaaat aaactgaaac
     3901 ggtcatgatc acacctccgc catccaccga ttacgccgcg tattacgaaa cctatatttc
     3961 gcttatcagg ggacgcgatc cgctgaaaat gctcgagtcg caggtgttcg atttcaaggc
     4021 gctgctgtcg gaaattcccg ctgaaaaaga agagttccgt tacgccgaag gaaaatggag
     4081 cgtgaaagag gtgatcggcc atatcaatga tacggaacgc gtgatgagct accgcgcgct
     4141 ttgcttcgca agaggagaga agaaagagct tccgggcttt gaggaaaatg attacgcaaa
     4201 agcgggaatg ttcgacaagc ggtcgttgta cgatctcggc cacgagtttg gtttcatccg
     4261 tgaagcgacg atcgcgcttt tcaaatcgat gagcgccgat gttctcgata accgcggcat
     4321 tgccaatcat aacacggtga caccgcgtgc gcttctttac attattgccg ggcatcacca
     4381 gcatcacgag cgtgtgctgc gtgaaagata tcttgcggat attctctgat catccgcgcg
     4441 gaaagt
//
LOCUS       DASTSF010000628         2990 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1922446,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000628 DASTSF010000000
VERSION     DASTSF010000628.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2990
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1922446"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..610)
                     /locus_tag="VFU15_17685"
     CDS             complement(<1..610)
                     /locus_tag="VFU15_17685"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEU4719683.1"
                     /translation="MQSKVKIMRLMLVFSTLLLCLKFFSWWLTKSNAILTDALESIVN
                     VVAGAFALFSISYAAKPRDKEHPYGHGKIEFVSAGFEGALIFFAGGVIIYEAIKGFIF
                     PHAIAKADIGAFLSAASGLCNFGMGYFLVKRGKKQHSALMEASGKHLISDTVSSIGLV
                     VGLYLIYVTGLSWIDNALAVTFGSYIIYTGFRIVRESLGNLLD"
     gene            complement(676..1215)
                     /locus_tag="VFU15_17690"
     CDS             complement(676..1215)
                     /locus_tag="VFU15_17690"
                     /inference="COORDINATES: protein motif:HMM:NF024952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HEU4719684.1"
                     /translation="METRLYFVALVPDPVVEQQVHAFKADLSDRFGTKAAMKSPAHIT
                     LFSPFRFSGNEQALFAALQEAANGMSAFPVKLENFDSFPPRVIFVDVHENELLMKLQR
                     KVKTVFLGAVKETKAAKDDFHPHMTIGNRDWSDGAFDDAWNEYRAKKFSASFTANRIT
                     LLRLDGAKWNVISEAPLTA"
     gene            complement(1248..2114)
                     /locus_tag="VFU15_17695"
     CDS             complement(1248..2114)
                     /locus_tag="VFU15_17695"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEU4719685.1"
                     /translation="MKKIISFSVLICLALCSAAFVFAKGGGKGKDKKPSDGVVRKYKS
                     KPVKKPVAVTLDSGVQYTITSKGDGPQIKKGDRVKVLYVGKLTNDTVFDASYLHNNVP
                     LPFHVGMHEVIPGWDSALVHLHGGDKATVRIPPSMGYGSMQRGKIPPNSTIIFEVEII
                     EICVKPTPWDAKGKDTITTPSGLKMVMYESHPDSIMPKNGNKVTVHYSGFLANDSMFD
                     SSVEREQPYTFVLGRDRVIKGWYEGITLMHKGEKAKLIIPYQLGYGESGIPPVIPPKA
                     TLIFDVQLLDVK"
     gene            complement(2121..>2990)
                     /locus_tag="VFU15_17700"
     CDS             complement(2121..>2990)
                     /locus_tag="VFU15_17700"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEU4719686.1"
                     /translation="GFEEQENGTYLLVHKKGTSEVLVDTGGAMFMKIRFLTEKDSVFV
                     DVNAASQTPSYPMRIDKPAFKGDFLDMLAKLHPGDSATFYVRLDSLKKHYPSEFKFEP
                     KFDTMKYLGFNVVVDSIYSRSKVEDFRKKAEAEQDKQMEEMRKVQAVMTPIQQKAKEM
                     EPELKKKDAQLLKPYLAQNKITVKPDADGIYYQETDPGDGTPLMPGMYVGMKYTGKYL
                     DGTIFDANTLVDGQEPYYFHLGQDPMIPGFTNCIMKMKNHGKAIFILPPKMGYNDGLT
                     RVFEVEITDAKKQ"
BASE COUNT          642 a          805 c          799 g          744 t
ORIGIN      
        1 cgtcgagaag attgccgagc gattcacgga cgatgcggaa acccgtgtaa atgatgtacg
       61 atccgaacgt gaccgcaagt gcgttatcga tccagctcag cccggtgacg tagataaggt
      121 aaagaccgac cacaagaccg atgctggaaa ccgtatccga aataagatgc ttcccgctcg
      181 cttccatgag cgcggaatgc tgcttcttcc cccgttttac gaggaagtaa cccatcccga
      241 aattgcacag gccggaagcc gcggaaagga acgcgccgat gtcggccttc gcgatcgcgt
      301 gcggaaaaat gaatcctttg atcgcttcgt agatgatcac gccgcccgca aaaaaaataa
      361 gcgcgccttc aaagccggcc gagacgaact cgatctttcc gtgcccgtac ggatgttctt
      421 tgtcgcgcgg tttcgctgcg tagctgatgc tgaacaaagc gaatgcaccg gccacgacat
      481 tgacgatgct ttccagcgcg tccgtcagga tggcattcga tttcgtcagc caccaggaga
      541 aaaatttcag gcagaggagc agcgtgctga ataccagcat cagccgcatg atcttcacct
      601 tgctttgcat caccgggttc acggaacgaa gttcgctgtt tacggattaa tgttcaacgc
      661 cgggatcgtt ttcttttatg cggtcagcgg cgcttcgctt atgacattcc attttgcgcc
      721 gtcgagacga agcagcgtga tcctgttggc agtaaaagaa gcggagaatt tttttgcgcg
      781 gtattcgttc caggcatcat cgaacgcgcc gtcgctccag tcgcggttac cgatggtcat
      841 gtgcggatga aaatcatctt ttgccgcttt ggtttctttc actgcgccga gaaaaaccgt
      901 tttgaccttc cgctgcagct tcatcagcaa ttcattttca tgcacatcga cgaagatcac
      961 gcgcgggggg aaagaatcga aattctcgag cttcacggga aatgcgctca tcccgttcgc
     1021 cgcctcctgc agagccgcaa aaagcgcctg ctcgttcccg ctgaaacgga aaggagagaa
     1081 aagcgtgatg tgcgccggtg atttcatcgc ggcttttgtg ccgaaacggt ccgaaagatc
     1141 cgccttgaag gcatgaacct gctgttcaac gactggatcc ggaacaagcg caacgaaata
     1201 gaggcgggtt tccataaaaa agacccgcag caggcgggtc gttgggctta ttttacatcc
     1261 aggagctgca cgtcgaagat gagcgtggct ttcggcggaa tgacaggcgg aattcccgat
     1321 tcgccgtaac cgagctggta cgggatgatc agcttcgcct tctctccttt gtgcatgagc
     1381 gtaatgcctt cgtaccagcc tttgatcacg cggtcgcggc cgagcacgaa cgtatacggc
     1441 tgctcacgct caacggatga atcgaacatg gaatcgttcg caagaaagcc gctgtagtga
     1501 accgtcactt tgttcccgtt cttcggcatg atggaatccg gatgcgattc gtacatcacc
     1561 attttcagtc cgctcggcgt ggtgatcgtg tcttttcctt tcgcgtccca cggtgtgggc
     1621 ttcacgcaga tctcgatgat ctccacctcg aaaataatcg tgctgttcgg cggaatcttt
     1681 ccgcgctgca tgcttccgta gcccatcgat ggcggaatgc gcacggtcgc tttgtcgccg
     1741 ccatgcaggt gcacgagcgc ggaatcccat ccggggatca cttcatgcat gcccacgtga
     1801 aaaggaagcg gaacgttgtt gtgcaggtag gacgcgtcga aaaccgtgtc attcgtcagc
     1861 ttgccgacat acagcacttt cacgcggtcg ccttttttga tctgcggacc gtcgcctttc
     1921 gaagtgatgg tgtattgcac gccgctgtcg agggtgaccg ctaccggttt cttcaccggc
     1981 ttcgacttgt atttgcggac gacgccgtcg gacggcttct tatcttttcc ttttccgccg
     2041 cctttcgcga aaacaaatgc agcggaacaa agggcgaggc aaatgagaac ggaaaaagaa
     2101 attatttttt tcatgaatgc ttactgtttt ttcgcatcgg tgatctcgac ctcgaacacg
     2161 cgggtaagtc cgtcgttgta gcccattttc gggggaagga tgaaaatggc tttcccgtgg
     2221 ttcttcattt tcatgatgca gttcgtgaaa ccggggatca tgggatcctg gccgaggtgg
     2281 aaataatacg gttcctgccc gtcgacaagc gtattggcat caaagatcgt tccgtcgagg
     2341 tatttgcccg tgtatttcat accgacatac atgcccggca tcagcggtgt tccgtccccg
     2401 ggatccgttt cctggtaata aataccgtcc gcatcgggct tgacggtgat cttgttctgc
     2461 gcgagataag gtttcagcag ctgcgcatcc tttttcttca gctcgggttc catttctttc
     2521 gctttctgct ggatcggcgt catcacggcc tgcaccttgc gcatttcctc catctgcttg
     2581 tcctgctccg cctccgcttt tttgcggaaa tcctccacct tgctgcggga atagatgctg
     2641 tcgaccacga cattgaagcc gaggtatttc atcgtatcga acttcggctc aaacttgaat
     2701 tcgctggggt aatgcttttt cagcgaatcc aggcgtacgt aaaacgtcgc gctgtcgccg
     2761 ggatgaagct ttgccagcat atcgaggaaa tcgcctttga aggccggctt gtcgatacgc
     2821 atgggataag aaggcgtctg cgaagccgca ttcacgtcga cgaagacgga atctttttcc
     2881 gtcaggaagc ggatcttcat gaacatcgcg ccgccggtat cgacgagcac ttcgctggtt
     2941 ccttttttat ggacgaggag atacgttccg ttttcctgct cttcaaagcc
//
LOCUS       DASTSF010000629         2829 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1570080,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000629 DASTSF010000000
VERSION     DASTSF010000629.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2829
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1570080"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(232..1620)
                     /locus_tag="VFU15_17705"
     CDS             complement(232..1620)
                     /locus_tag="VFU15_17705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010609504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HEU4719687.1"
                     /translation="MRHNLLEACYLIGSVTFIIGMKLMGNAKTARRGNLIGAFGMTIA
                     ILGTVLLHEGEVAGMIYGLIAGAVALGTVIGWMTAKKVQMTKMPELVSMFNGMGGACA
                     ALIGLMEYEHNIGQTGALIAIVAGTVIGSISFSGSIIAFLKLNGTMNKPIRLPAYNVI
                     NTIVMIGVFAFAGWMVYASPEGHTMLYALFGCAIVYGILFTVPIGGADMPVVISLLNS
                     FTGLAAAFGGFLYGNYVMLTGGILVGSAGTLLTLEMCKAMNRPLSNVIFGAFGGGGGA
                     AAAGDQTKGSIKDISVSDLAILMNYSKKVVIVPGYGLAVAQAQHVIHELEMLLEERGV
                     EVKYAIHPVAGRMPGHMNVLLAESNVAYEKLVEMDDINPEFEQTDVVLIVGANDVVNP
                     AAKTDPSSPIYGMPILEVDKAKNIIVNKRSMNAGYAGIDNLLFYNPKCSMFFGDAKKA
                     LTELVAELKTLD"
     gene            complement(1666..1983)
                     /locus_tag="VFU15_17710"
     CDS             complement(1666..1983)
                     /locus_tag="VFU15_17710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015029219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HEU4719688.1"
                     /translation="MDTIRDIFTFFNEHKDIFYFVVLSIFVGVEVIGGVPTVLHTPLM
                     SGANAIHGVVIVGAIHVMLNVDPTNYVALSLGFLAVLLGTLNVVGGFVVTDRMLEMFK
                     KKK"
     gene            complement(2020..>2829)
                     /locus_tag="VFU15_17715"
     CDS             complement(2020..>2829)
                     /locus_tag="VFU15_17715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HEU4719689.1"
                     /translation="CKAGVSAFSMDAIPRISRAQKMDALSSQANLAGYKSVLMAADTI
                     GKILPMMTTAAGTIRPSRIVIFGAGVAGLQAIATAKRLGGVVEVSDIRPETKEQVESL
                     GGKFIEVKGDDSVKMEGGYVKGVSEEFLKKQQELVSKHVKDADIVITTALIPGRKAPL
                     LVTEEMVKSMRFGSVIVDMAVEQGGNVVGSELNQTVVKHGVTIIGESNIPSLLPMNAS
                     DLYAKNIATFLYHLADKDKFKWEMDEEITKGSLIVHDGVAVHPSVAKAVNA"
BASE COUNT          661 a          757 c          741 g          670 t
ORIGIN      
        1 ataagatacc tatcaaacgg cttttccttt ttgccagttt cgccacgaga ttgttgctca
       61 tcctgacact gagccataga taggcggcat aaataacaat gaagattaaa gcataaacaa
      121 atttcatggg attatttttt aaaaatgata gtatataaat atataacata taccatgcaa
      181 aaaaacgccc gccgtttccg gcgggcgctc aacatctttg aggtataaaa attaatcgag
      241 cgtcttcagc tcggccacca gctcggtcag cgctttttta gcgtcgccga agaacatgga
      301 gcatttcggg ttgtagaaaa gcaggttgtc gatgcccgcg taacccgcgt tcatggaacg
      361 cttgttgacg atgatgttct ttgctttatc cacttcgagg atgggcatgc cgtagatcgg
      421 tgaagaagga tccgtcttcg cagcggggtt aacgacgtcg tttgcaccga cgataagcac
      481 cacatccgtc tgctcgaatt caggattgat gtcgtccatt tccaccagct tctcatacgc
      541 cacgttcgat tccgcgagaa gcacgttcat gtgaccgggc atacgtcccg ccacagggtg
      601 aatggcatat tttacttcca cgccgcgttc ttcgaggagc atttcaagct cgtggatgac
      661 gtgctgcgcc tgcgcaacgg caagaccata accgggaacg ataacgactt tctttgaata
      721 gttcatcagg atcgcaaggt cgctgacgga aatgtccttg atggaacctt tcgtctggtc
      781 gccggctgcg gcagcaccgc cgccgccgcc gaatgcgccg aagatcacgt tgctaagcgg
      841 gcggttcatc gccttgcaca tttcgagtgt gagcagcgta cctgcagatc cgacaaggat
      901 accgccggtg agcatgacgt agtttccata caggaaacca ccgaaagctg cggcaagtcc
      961 ggtgaacgag ttaagcagtg aaataaccac cggcatatcc gcgccgccga tcggaaccgt
     1021 gaacagaatt ccgtaaacga tcgcgcagcc gaagagcgca tacagcatcg tgtgtccttc
     1081 gggagaggcg tacaccatcc atccagcgaa agcgaaaacg ccgatcatta cgatcgtgtt
     1141 gatgacgttg tacgcaggca ggcggatcgg cttgttcatc gttccgttca gcttgaggaa
     1201 cgcgataatg gaaccggaga aggaaatgct gccgatcacg gttcctgcca caatggcgat
     1261 aagcgcaccg gtctggccga tattgtgctc gtactccatc agcccgatga gcgcagcgca
     1321 ggcgccgccc atcccgttga acatggaaac gagctcgggc attttcgtca tctggacttt
     1381 cttcgccgtc atccacccga taacggttcc gagcgccact gcgcccgcga taagtccgta
     1441 gatcattccc gccacttcgc cttcgtgaag aagaaccgtg ccgagaatgg cgatggtcat
     1501 gccgaacgca ccgatgaggt ttccgcggcg cgccgttttt gcgttgccca tcagcttcat
     1561 cccgatgatg aacgtcaccg atccgatgag ataacaggct tcgaggagat tatgtcgcat
     1621 aaacaatttt ggaatttgga attttatttt tgggagcttg ggtcgttatt ttttcttctt
     1681 gaacatttca agcatgcggt ccgttacgac gaatccgccg accacgttca gcgttccgag
     1741 caggacagcg aggaagccga gcgaaagtgc tacgtaattc gtgggatcga cgttgagcat
     1801 gacgtgaatg gcgccgacga tgacgacacc gtggatcgcg ttggcgcccg acatcagtgg
     1861 ggtgtgaaga acggtcggta cgccgccgat cacttcaacg ccgacgaaga tggaaagcac
     1921 aacgaaatag aaaatatcct tgtgctcgtt gaagaaagtg aaaatgtctc tgatagtatc
     1981 cattctgtat tgagttggga agttgaaaag tttgaaagtt tatgcgttca cggcttttgc
     2041 caccgaagga tgtacggcaa cgccgtcatg aacgatgagc gatcctttcg tgatctcctc
     2101 gtccatttcc catttgaact tgtccttgtc ggccaggtga tacaggaacg tggcgatgtt
     2161 tttcgcgtac agatcgctcg cgttcatcgg gagaagcgaa ggaatattgc tttcgccgat
     2221 gatggtgaca ccgtgtttta ccacggtctg gttcagctcg ctgccgacga catttccgcc
     2281 ctgctccacc gccatgtcga cgatcacaga accgaaacgc atgctcttca ccatttcttc
     2341 cgtgacgagc aacggtgctt tcctgccggg aatgagcgcg gtggtaatga cgatgtccgc
     2401 gtctttcaca tgcttcgaaa caagctcctg ctgttttttc aggaattctt ccgaaacgcc
     2461 ttttacatag ccgccttcca tcttcacgga atcgtcgcct tttacctcga tgaattttcc
     2521 gccgagcgat tccacctgct ctttcgtttc gggacggatg tcggaaactt ccaccacacc
     2581 gccgagacgt ttcgccgtgg cgatcgcctg cagaccggcc acaccggctc cgaagatcac
     2641 gatcctggaa ggacgtatcg ttccggccgc ggtggtcatc atcgggagaa ttttcccgat
     2701 cgtgtcggcc gccatgagca cggatttata accggcgagg ttcgcctgcg aggaaagcgc
     2761 atccatcttc tgcgcccgtg aaatacgggg gatcgcatcc atggaaaacg cggaaacacc
     2821 ggctttgca
//
LOCUS       DASTSF010000630         3046 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_536396,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000630 DASTSF010000000
VERSION     DASTSF010000630.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3046
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_536396"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1110
                     /locus_tag="VFU15_17720"
     CDS             <1..1110
                     /locus_tag="VFU15_17720"
                     /inference="COORDINATES: protein motif:HMM:NF025616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HEU4719690.1"
                     /translation="PALPSIKKTPDMKTHHPFLLTALSLSLIIYSCASDEAKRKSYDE
                     NAPDKSYSNSAAMDSTVSTNRYAPQQTQEYSGSSGVTGGNQNESTDKSMAADEENFAF
                     RKIFASSAASLSKLDSTHSFVRTADLKFRVKNTAAATYRIEDIVRHFGGWVANTQLES
                     SVYDERTTRISEDSSLVTTRFVVNNSMTLRIPATNLDTFLKAMVPFVDFFDHRTINVN
                     DITLDLLANRLEQERNAKFSKRFSADIDTKPAKLASVQDAEESLLNNQAASDNALIQN
                     LRMADQVNYSTVTMSIYQKESIRQELVANEKSYESYEPGLGTKLGHAFVSGWRGLKII
                     LIILVTLWPFWVFGGLTWYLVRWGLKKRKASETKS"
     gene            complement(1178..2266)
                     /locus_tag="VFU15_17725"
     CDS             complement(1178..2266)
                     /locus_tag="VFU15_17725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719691.1"
                     /translation="MNRDGKKNVPAPDVRGMFAGFFTDGNGKYADSFPEQVLEKIREE
                     KLFKCFLPESLGGLALGLREMLKIIEDSSYLNGSFGWLVQIGNGGNYFAACFDERVSQ
                     ELFSPASAVLAGSGAPSGKAIPVDGGYRVSGEWKYCSGAAYATFFTMNCIVEGSGEMI
                     SCATMRENVEVISDWKTFGLKNTSTDSMRVKDVFVPREFVFSYMEHKSYHDVPIFRLP
                     FLAYAQLFFMPVVFGIFQRYAEESLAYSSAQEKFWTRSHPGRTEYIAKKCRSFLRDLA
                     SFRSRLFELAEKIESSSGVQQEEFCAALDRESRTHNRALVQYAHELFPLLGMQALYLD
                     HPLNIFYTDLLCASQHKLLNSYSTETAE"
     gene            complement(2263..2562)
                     /locus_tag="VFU15_17730"
     CDS             complement(2263..2562)
                     /locus_tag="VFU15_17730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014678805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEU4719692.1"
                     /translation="MKKYFFLKLNPPRSSFMTDMNEEERSIMQHHVDYWKPHVAAGTM
                     MVMGPVMDPKGGFGVGIVCVSDESELDELVGNDPANGLNSYEIHPMRVVSKFISA"
     gene            2663..>3046
                     /locus_tag="VFU15_17735"
     CDS             2663..>3046
                     /locus_tag="VFU15_17735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019586667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HEU4719693.1"
                     /translation="MSPISAQNWKQVEDYLRRDDRCILPIGSTEQHAFLSLSTDTVLA
                     EKIAQDAAEEEGVPVFPVLPYGITPGFMAYPGTVTLKTETLTRVVAEILGSLAHHGFR
                     RIMIVNGHGGNRALEETLSDFLSRHP"
BASE COUNT          734 a          864 c          774 g          674 t
ORIGIN      
        1 cccgctcttc cgtccattaa aaaaacgcca gacatgaaaa cccatcatcc cttcctgctg
       61 acggcgctgt cgctgtcgct gatcatttat tcctgcgctt ccgacgaagc caaacgaaaa
      121 tcctacgacg agaacgcccc ggataaatcc tacagcaatt ctgcggcaat ggattccacc
      181 gtttcaacta atcgttacgc cccgcagcag acccaggaat acagcggctc ttccggtgtt
      241 acgggcggga atcagaacga atccaccgat aaatccatgg cggctgatga ggagaatttt
      301 gcgttccgga agatcttcgc ttcttccgcc gcttcgctgt cgaagctcga cagcacgcac
      361 agcttcgtgc gaacggccga cctgaaattc cgcgtaaaaa atacagccgc cgcgacgtat
      421 cgcatcgaag atattgtgcg gcatttcggc ggatgggtgg cgaacacaca gctggaaagt
      481 tccgtttacg acgaacggac aacccgcatc agcgaagaca gctcgctcgt gactacgcgc
      541 tttgtggtga acaacagcat gacgctgcgc attcccgcca cgaacctcga cacgttcctg
      601 aaagcgatgg ttcccttcgt ggatttcttc gatcaccgca ccatcaatgt caacgatatc
      661 acgctcgacc tgctggcgaa ccgcctcgag caggaacgca atgcgaagtt cagcaagcgt
      721 ttttcagccg acatcgacac gaaaccggcg aagctcgcca gcgtacagga tgcggaagaa
      781 agcctgctga acaaccaggc cgcgtcagat aatgcgctga tccagaacct gcgcatggcc
      841 gaccaggtca attacagcac cgtaacgatg agcatttacc agaaggaaag catccggcag
      901 gaacttgtcg ccaatgaaaa aagctatgag tcgtacgagc cgggacttgg aaccaagctg
      961 ggacatgcgt tcgtttccgg ctggcgcgga ctgaagatca tccttattat tctcgttaca
     1021 ctgtggccgt tctgggtttt cggcggactg acctggtacc tggtgcgctg gggactgaaa
     1081 aaaagaaaag cttctgaaac caaaagctga ggagtaagcc gccatcagtc tttcgtgcat
     1141 tctttttcaa agattcaagc cgcgatcagt tgcgtattta ttctgcggtt tcagtggaat
     1201 agctgttcag caatttatgc tgcgacgcgc aaagcaggtc ggtgtaaaaa atattgagcg
     1261 ggtgatccag gtaaagcgcc tgcatgccga gcaacggaaa aagttcgtgc gcatattgca
     1321 ccagcgcgcg gttgtgcgtg cgtgattcac ggtcgagcgc agcgcagaat tcctcctgct
     1381 gaacgccgga agaactttca attttttcag ccagctcaaa gagacgcgaa cggaatgaag
     1441 cgagatcgcg gagaaatgac ctgcattttt tcgcaatgta ttccgtcctg ccgggatgcg
     1501 atcgcgtcca gaatttttcc tgcgcgctgc tgtaggccag cgactcttcc gcgtaacgct
     1561 gaaaaatccc gaaaacgaca ggcatgaaaa acagctgcgc atacgcaaga aacgggaggc
     1621 ggaagatcgg cacgtcgtgg tagcttttgt gctccatgta gctgaacacg aattcccgcg
     1681 gaacgaaaac atccttcacg cgcatggaat ccgttgacgt gtttttcagg ccgaatgttt
     1741 tccagtcact gatgacttcg acattttcgc gcatcgttgc gcaggagatc atttcgccgc
     1801 tgccttcgac gatgcagttc atggtgaaga acgtcgcgta ggctgcgccg ctgcaatatt
     1861 tccattcacc ggaaacgcgg taaccaccgt cgaccgggat cgcttttccg gaaggagcgc
     1921 cgctgccggc aagcaccgca ctggcgggtg aaaaaagttc ctgtgaaacg cgctcatcga
     1981 agcaggccgc gaaataattt ccaccgttcc cgatctgcac cagccatccg aagctcccgt
     2041 tgaggtatga gctgtcttcg atgatcttca gcatttcgcg gagacccagc gcgagtccgc
     2101 caaggctttc cgggagaaaa catttgaaca gcttttcttc gcggatcttt tccagcacct
     2161 gctccggaaa agagtcggcg tattttccgt tcccgtccgt aaaaaaaccc gcgaacattc
     2221 cgcgaacatc cggcgcaggc acgttcttct tcccgtccct gttcacgcag aaataaattt
     2281 tgacaccacg cgcatcgggt gaatttcata gctgttcagc ccgttcgccg gatcgttccc
     2341 cacgagctcg tccaactcgc tttcgtcgct gacacagaca ataccgacac cgaacccgcc
     2401 cttcgggtcc atcaccggtc ccatgaccat catcgttccc gctgcgacat gcggtttcca
     2461 gtagtcgaca tgatgctgca tgatgctgcg ttcctcctca ttcatatctg tcatgaaaga
     2521 cgatctcggc ggattaagtt tcagaaaaaa atattttttc atacccgttg tttttgccgg
     2581 gaaatgtttc ccgggaaggc ggaaaattac gaatgttccg cgcagcccgt tatttccgtt
     2641 attttcactt caaaaattta ccatgagccc tatttctgca cagaactgga agcaggtgga
     2701 agactacctc cgccgcgacg accgttgtat tctcccgatc gggtcgacgg aacaacacgc
     2761 gttcctcagt ctttcgaccg acacggttct cgccgaaaag atcgcacagg atgctgcgga
     2821 agaagaaggc gtcccggttt tccccgtatt accttacggc atcacacccg gcttcatggc
     2881 ctacccggga accgtgacac tgaaaacgga aacgctgaca agagtggtgg cggaaatact
     2941 cggcagcctt gcgcaccatg gtttccgccg catcatgatc gtgaacgggc atggcgggaa
     3001 ccgcgcgctg gaagaaacgc tttctgattt tttgtcgcgg cacccg
//
LOCUS       DASTSF010000631         3744 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1698067,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000631 DASTSF010000000
VERSION     DASTSF010000631.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3744
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1698067"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1272)
                     /gene="lysS"
                     /locus_tag="VFU15_17740"
     CDS             complement(<1..1272)
                     /gene="lysS"
                     /locus_tag="VFU15_17740"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003007410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEU4719694.1"
                     /translation="MNPELSEQENVRRQKLAELRSLGIDPYPAEEFKVNVTAADIHEN
                     YERDKTNYKDVSIAGRLMGMRIIGKASFAELQDATGRIQLYVNRDEICPGEDKTLYNT
                     VFKKLLDIGDIIGISGYVFTTQVGEISIHVTSLKLLSKSLKPLPIVKVDAEGTAHDEV
                     SDMEFRYRQRYADLVINPSIKETFLKRTKMINTMREFLNDKGLLEVDTPVLQAIPGGA
                     AARPFITHHNALDVPFYLRIANELYLKRLIVGGFDGVYEFSRNFRNEGMDRTHNPEFT
                     ILEFYVAFRDYNWMMRTCEEMLEKVAVALHGTTEVPVGENKINFNAPFRRITLYDAIK
                     EYAGVDVSSMDEAQLRDTCRHLGVEVDKSMGRGKLIDKIFGDKCEGHYIQPTFITDYP
                     VEMSPLTKKHRSKEGLVERFELMVNGKEIANA"
     gene            complement(1539..3359)
                     /gene="uvrC"
                     /locus_tag="VFU15_17745"
     CDS             complement(1539..3359)
                     /gene="uvrC"
                     /locus_tag="VFU15_17745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020080020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEU4719695.1"
                     /translation="MSTEEQLRQNIKALPDSPGVYQYFDAEGKLLYVGKAKNLKKRVA
                     SYFTKEHDNAKTAILVRRIADIRYIVVDSELDALLLENSLIKKHQPRYNVMLKDDKTY
                     PWICITKERFPRVFHTRKLVRDGSEYFGPYASVKVMYTMLDLVKKMYPLRNCRLVLSE
                     KNIAEKKFRVCLEFHIGNCLGPCEGLQSEEDYNNSIAHIRSILKGNISAVIRQLKELL
                     QDAVAKLEFERAHDLKENIDALETYRGKSTVVPSNITNVDVFTIQSDEKAAYVNFFKV
                     IDGAIVQGHTIEMKKKIEESDEELLQLAIVELRERFSSNAPEVVVPFPVSLEIPGVEF
                     HVPQRGDKRKLIELSERNVEYFRKEQEKRESLTDPERHTKRILATMMKDLRLKEEPRL
                     IECFDNSNFQGDFAVSAMTVFRDARPSKKEYRHFNVKTVEGPDDFATMEEVIFRRYKR
                     VQEENLDMPQLIVIDGGKGQLHAAMNSLEKLGLRGKVAVIGIAKRLEEIYYPGDPVPL
                     YLDKKSETLRIIQHIRDEAHRFGITHHRKKRSKGTIKTGLTEIKGISDATAKKLLSEF
                     KSVKQLKEKSEEEIAAVIGKAKGKLVWEYFRAETGKPGAG"
     gene            complement(3397..>3744)
                     /locus_tag="VFU15_17750"
     CDS             complement(3397..>3744)
                     /locus_tag="VFU15_17750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719696.1"
                     /translation="YLLLKRRLHSWAILSTFAAILYLCASWWAYRFDCAFGYRSFVEY
                     YPLFIIPVAALIEKLLKPGSAWWKPTLFFLLATFFIYVNMRLCLGPAQLTWCSKEYFW
                     PDYWKMMQTIYTF"
BASE COUNT          739 a         1040 c         1007 g          958 t
ORIGIN      
        1 ggcgttggca atctctttac cgtttaccat cagctcgaaa cgctccacga gaccttcttt
       61 gctgcggtgc ttcttcgtaa gcggcgacat ttccacggga taatccgtga tgaacgtcgg
      121 ctggatgtaa tggccttcgc atttgtcgcc gaaaattttg tcgatcagct ttccgcgtcc
      181 catggatttg tccacttcca cgccgagatg acggcaggtg tcgcgcagct gcgcttcgtc
      241 catgctgctc acgtccacgc ccgcgtattc tttgatcgca tcgtacaggg tgatgcggcg
      301 gaacggtgcg ttgaagttga tcttattttc gccgacagga acttccgttg tgccgtgcag
      361 tgcgaccgcc actttctcga gcatttcctc gcacgtgcgc atcatccagt tgtaatcgcg
      421 gaacgccaca tagaattcga ggatcgtgaa ttcggggttg tgcgtacggt ccattccttc
      481 gttgcggaag ttgcgcgaga attcgtacac accgtcgaag ccgcccacga tgaggcgctt
      541 caggtaaagt tcattggcga tgcgcaggta gaacggaaca tcaagcgcgt tgtggtgcgt
      601 gatgaacggg cgcgctgctg cgccgccggg aatggcctgc agcaccggcg tgtccacttc
      661 gagaagtcct ttgtcgttca ggaactcgcg catcgtgttg atcattttcg tgcgcttgag
      721 aaacgtttcc ttgatggaag gattgatcac gagatcggcg tagcgctggc ggtaacggaa
      781 ttccatgtcg gaaacttcgt cgtgcgctgt accttctgca tccactttca caatgggcag
      841 cggcttgagc gatttgctga gcagtttgag cgatgtgacg tgaatggaaa tctctcccac
      901 ctgcgtggta aacacgtagc cggaaatgcc gatgatgtcg ccgatgtcca gcagcttttt
      961 gaacacagtg ttgtacagcg tcttgtcctc gcccgggcag atctcgtcgc ggttcacgta
     1021 tagctgtatc cgccctgtgg catcctgcag ctccgcaaat gaagctttgc cgataatgcg
     1081 catgcccata agccgtcccg ctatgctcac gtccttgtaa ttcgtcttgt cgcgctcata
     1141 gttttcgtga atgtcggctg ccgtgacgtt cactttgaat tcttccgcgg gatacggatc
     1201 gatacccagg ctgcgcagct cggccagctt ctgccggcgt acattttcct gttctgaaag
     1261 ttctggattc attggtctgg taaagtgcgg caaagataga ggatagttcc cagttgggga
     1321 atggagaacg gaacacggag gttgcagcag ggaagcgcag cggcagtgca tttctgttcc
     1381 tgtgttcccg tcttccgctt gtactccgca gcgcaccgtc aggccgcgcg caaatcgaag
     1441 cccggtgtgc tgcggggtat cgcttcagcc ggggctaacc gggtgcctgc tgttcagcaa
     1501 atgcttgaat actggatctt aaacccggaa cattgaactt acccggcacc gggtttcccg
     1561 gtctccgccc tgaaatattc ccacaccagc tttcctttcg ctttcccgat gacggccgca
     1621 atctcctctt ccgatttttc cttcagctgt ttcacggatt tgaattccga caataatttt
     1681 ttcgccgttg catcgctgat gccttttatt tccgtcagcc cggttttgat ggttcccttg
     1741 ctccgtttct tgcggtgatg cgtgatcccg aaacggtgcg cttcgtcgcg gatgtgctgg
     1801 atgatgcgca gcgtttccga ttttttgtcg agatacagcg ggaccgggtc gccgggataa
     1861 taaatttctt caagacgctt cgcaatgccg atcacggcca ctttcccgcg cagcccgagc
     1921 ttctccagcg aattcatcgc cgcatgcagc tgtcctttcc ccccgtcgat cacgatgagc
     1981 tgcggcatat cgagattttc ttcctgcacg cgcttgtagc ggcggaaaat gacttcttcc
     2041 atcgtcgcga aatcgtcagg gccttctacg gttttcacgt tgaaatgccg gtattctttt
     2101 ttgctcgggc gtgcatcgcg gaaaaccgtc atcgccgaaa ccgcgaaatc gccctggaaa
     2161 ttggaattgt cgaagcactc gatcaggcgg ggctcttcct tcaggcgaag atctttcatc
     2221 atcgtcgcca ggatgcgttt cgtatgccgt tcgggatcgg tgaggctttc ccgtttttcc
     2281 tgctccttgc ggaaatattc gacgttgcgt tcggaaagct cgatgagctt gcgcttgtcg
     2341 ccgcgctgcg gaacatggaa ctcaacgccc ggaatttcca gcgaaacggg aaaaggaacc
     2401 accacttccg gcgcgttgct gctgaacctt tcgcgcagct ccacgatcgc cagctgcagc
     2461 agctcttcat ccgattcttc gatcttcttc ttcatctcga tcgtatgtcc ctgcacgatc
     2521 gcaccgtcga tcactttgaa aaaattcaca tacgccgctt tttcatccga ctgtatcgtg
     2581 aacacatcca cgttcgtgat gttcgacgga acgacggtcg attttccccg gtaggtttcc
     2641 agcgcgtcga tgttctcttt caggtcgtgc gcacgttcga attccagctt cgccacggca
     2701 tcctgcaaaa gctctttcag ctggcggatc accgcactga tgttcccttt caggatgctg
     2761 cggatatgcg cgatgctgtt gttgtagtct tcctcgctct gcagcccttc gcacggaccc
     2821 aggcaattcc cgatgtggaa ttcgaggcac acgcggaact ttttctccgc aatgttcttt
     2881 tcgctcagca cgaggcggca gttgcgcagc gggtacattt tcttcaccag gtcgagcatg
     2941 gtgtacatca ccttcacgct ggcgtacgga ccgaaatatt ccgagccgtc acgaacgagc
     3001 ttgcgcgtgt ggaagacgcg cgggaaacgt tctttcgtga tgcagatcca cgggtacgtt
     3061 ttgtcgtcct tcagcatcac gttgtaacgc ggctggtgtt tcttgatcag cgaattttcc
     3121 agcagcagcg catccagctc ggaatcgaca acgatatagc ggatgtcggc gatcctgcgc
     3181 acgagaatgg ccgtcttcgc gttgtcgtgc tctttcgtaa aataggaagc aacccgcttc
     3241 ttcaggttct tcgctttccc gacatacagc agctttcctt cagcatcaaa atactggtaa
     3301 acgccggggg aatcgggcag cgcttttatg ttctgtcgga gctgttcttc cgtgctcatg
     3361 caggacaaag ataggacagg aaaacacgct gctttttcag aacgtgtaga tcgtctgcat
     3421 catcttccag taatccggcc agaagtattc tttcgagcac cacgttaatt gcgcgggacc
     3481 gaggcagagg cgcatgttca cgtaaatgaa aaaggtggcc agcaggaaaa agagggttgg
     3541 cttccaccag gccgaacccg gcttcagcag cttttcaatc agcgcggcaa caggaatgat
     3601 gaaaagcggg taatattcca cgaagctgcg gtagccgaat gcgcagtcga accggtacgc
     3661 ccaccagctg gcgcagaggt aaaggatcgc ggcgaacgtg gaaaggatcg cccacgaatg
     3721 caggcggcgc ttcagcagga gata
//
LOCUS       DASTSF010000632         5690 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_137841,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000632 DASTSF010000000
VERSION     DASTSF010000632.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5690
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137841"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1571
                     /locus_tag="VFU15_17755"
     CDS             <1..1571
                     /locus_tag="VFU15_17755"
                     /inference="COORDINATES: protein motif:HMM:NF017393.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M43 family zinc metalloprotease"
                     /protein_id="HEU4719697.1"
                     /translation="VKTIGTAGVAGYAYLPGTAPSSTTDGILILSSYIGSIGSSTASH
                     SRSLTHEIGHFLNLKHVWGNTNTPSTDCGATGTCGDDGVNDTPHTKGWGFCCNLANDD
                     ICVPGTHENVQNYMEYSYCSCMFTAGQVTRMRSALTSATAQRSSLWTASNLVATGVSN
                     NPPTVCKPNADFVPNNITMVCAGSSVTFTDASWNGHPTSWNWSFPGGTPATSTDSIPV
                     IQYNTPGTYNVTLTASNSSGSSTTTKNGIVIVSANTAQYNSWQYYEGFESATTFTSDW
                     IVNNPEGNGWARSTAAAYTGTGSARLANTSSMGGQVDELISPSIDFSAIGSTTMTFRI
                     AYAQRTSTSADKLRVLVSTNCGASWTQRYVKIGATLSTATATTSNFMPNSSQWRQETL
                     NLSNLATQTNVRIKFEFTSDGGNNIYLDDINLTGPSAVSTPDAAIQNFDVFPNPVNDN
                     TLISFSLQDKEKVDLSIYDMTGREVMTVYSGDLDAGDHQFPLNTSGTLSPGVYLVRMV
                     TVSGRSVTQRMIVD"
     gene            complement(1639..2076)
                     /locus_tag="VFU15_17760"
     CDS             complement(1639..2076)
                     /locus_tag="VFU15_17760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719698.1"
                     /translation="MKLRATLLASLFTLAVASDTVAQCNKTWVRKKCVPKIAPFIHNG
                     QMNSTLLKEGGKMETVLTFYSGQDYRILVCAEETLENVSFTVSDMTGKIVFDSKQHNN
                     TDVWDFKVKTTMDLKVDVDAGTNEGGDGNATGCVSILVGFKTK"
     gene            complement(2149..3057)
                     /locus_tag="VFU15_17765"
     CDS             complement(2149..3057)
                     /locus_tag="VFU15_17765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719699.1"
                     /translation="MKRISAFVSVPVISFALIACSGSNSPGGANDNGNDSSSIEVNQD
                     VVSAENVFVYVPSPIETAGLLKEAGAKYNKDFLNLPQNASKYSTTASLALNLGVYGAD
                     LAFAGIFDQHAETMVYLDCTGKLADGLHVSTAFSTDRKNRLEANMNNRDSVLSIITDS
                     YWDCDALLHENKEEYASSLMVAGGWVEGLYLACKVAEQTNSNDIRVRIVEQRYSLDKL
                     LVLLGREKNEDVKSVTGQLRELKVLFDALPKNPDQPTVVSTDDKSGMTTLGTDPAPPP
                     VSLNALEFKNILERVTVIRDSIITRN"
     gene            complement(3147..>5690)
                     /gene="polA"
                     /locus_tag="VFU15_17770"
     CDS             complement(3147..>5690)
                     /gene="polA"
                     /locus_tag="VFU15_17770"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEU4719700.1"
                     /translation="LDAFNIATLESDGFEADDVIGTLAKKAEKKHFITYMMTPDKDYG
                     QLVSHHTFIYKPGYKGGAPEKLGSEEICAKYGIKHPEQVIDILGLMGDSVDNIPGIKG
                     VGEKTALSLIHEFGSIDNLLRNTDKLKGKLKEKVEQGKEQALFSRKLATIITDAPVEL
                     DEEQLKLKEVDREKVKQLFSELEFRRLAQTVLGEELPAGETEKEEENGNPEKTASRKT
                     KPGQIDIFGNTGGEPSVPEAGENTSFKTIENVTHHYHVADTKEKRAALVAHLEKLSSF
                     CFDTETTGLDTMTASIVGMSFSWKKHEGWYVPVPAEEKEAQEVMEEFRAVFENKKIAK
                     TGQNIKFDMNVLRRYKISVQGELFDTMIAHYLLHPDMRHNMDLLSETYLGYSPVSIET
                     LIGKRGKNQLTMRDVPLEKISEYAAEDADVTWQLQDVFEPQLKEAGAEKLFRDIEIPL
                     VPVLADMENEGIAIDTAVLKKYSAELENEIVDVDKEIQQLAGTPFNISSPKQVGDILF
                     EVLKIGGKPKKTKTGQYATGEDVLQKLVHEHPIVEKILTYRELVKLKNTYVDTLPEMV
                     NPATGRIHTTFNQVVAATGRLSSDNPNLQNIPIRTARGREIRKAFIPRDHHHVLLSAD
                     YSQIELRIVASISGDPNMCKAFRDGVDIHTATAARVYGVDEKEVTKEMRYKAKSVNFG
                     IIYGQGAFGLAENLGIPRAEAKEIIDNYKKEFPGIQKYMDNMINFAREHGYVQTICGR
                     RRYLRDINSANAVVRGFSERNAINAPIQGSAADMIKLAMIHVHRELEKHRFRTKLLLQ
                     VHDELVFDVPKEEVNDVKPVVETCMKKALELDVPVEVGIGTGNNWLDAH"
BASE COUNT         1179 a         1599 c         1453 g         1459 t
ORIGIN      
        1 tcgtgaaaac gatcggtacc gcaggcgtgg ccggttatgc atacctgccc ggcacggcgc
       61 cttcttccac gacagacggc attctcatcc tttcaagcta catcggcagc atcggttcga
      121 gcacggcgag ccattcacgt tcgctcacgc acgagatcgg ccacttcctg aatcttaagc
      181 acgtatgggg aaacaccaac accccgagca ccgactgcgg agccaccggc acctgcggcg
      241 atgacggtgt aaacgatacc ccgcatacca aaggctgggg attctgctgc aatcttgcga
      301 acgatgacat ctgcgttccc ggcacacacg agaacgtgca gaactacatg gaatattcct
      361 actgctcctg catgttcacc gcaggacagg ttacgcgcat gcgcagcgca cttaccagcg
      421 ctaccgcgca gcgaagctcc ctctggacag cgtcgaatct cgtggcaacc ggagtgagca
      481 acaacccgcc aacggtctgc aagcctaacg cggattttgt tcccaataac atcacgatgg
      541 tgtgcgcagg atcttcggtc acgtttaccg acgcgtcctg gaacgggcac ccgaccagct
      601 ggaactggtc tttccccgga ggaacacccg ccacgagcac ggattcgatc ccggtcatcc
      661 agtacaatac accgggaacg tataacgtaa cgctcacggc ttccaactcg tccggttcgt
      721 ccactacaac gaaaaacggc atcgttatcg tttcggcgaa taccgcgcag tataattcct
      781 ggcagtatta cgaaggattt gaaagcgcaa cgacattcac cagtgactgg atcgtcaaca
      841 acccggaagg caacggctgg gcccgttcca cggcagctgc atacacggga acaggatcgg
      901 cgagactggc gaacacctct tccatgggcg gacaggtgga tgagctgatc agtccttcca
      961 tcgatttttc ggccatcggt tccaccacga tgacattccg catcgcttat gcgcagcgta
     1021 cttccaccag cgcggacaaa ctccgcgtgc tcgtctccac caattgcggc gccagctgga
     1081 cgcagcgtta cgtgaagatc ggcgccacgc tttccacggc taccgctacc accagcaact
     1141 tcatgccgaa ctcttcgcag tggagacagg aaacgctgaa tctttccaac ctcgccacgc
     1201 agacgaatgt tcgcatcaag ttcgagttca ccagcgacgg cggaaacaat atttacctcg
     1261 acgatatcaa tcttacaggt ccgtcggcgg tgagcacgcc tgatgcggcg atccagaatt
     1321 ttgacgtgtt cccgaacccg gtcaatgaca atacgctgat ctctttcagc ctccaggaca
     1381 aggaaaaagt cgatctcagc atctatgaca tgaccggccg tgaagtgatg acggtttatt
     1441 ccggcgacct cgatgcaggt gaccaccagt tcccgctgaa cacaagcgga acgctttctc
     1501 ccggcgtata tcttgttcgc atggtaaccg tttccggaag aagcgttacc cagcgcatga
     1561 tcgtcgactg atgacaggac aacaggaaaa agaaaaggcg tcacatttac gtgacgcctt
     1621 ttttatgaaa gagtcttttt atttcgtctt gaacccaacg aggatcgaaa cacatcctgt
     1681 cgcatttccg tctccgcctt cattcgtgcc ggcgtccaca tcgaccttaa gatccattgt
     1741 cgttttcact ttgaaatccc acacatccgt gttgttgtgc tgcttgctgt cgaaaacgat
     1801 cttccccgtc atgtcactca cggtgaagga aacattttca agcgtttctt cggcgcatac
     1861 cagtatcctg taatcctgcc cggaataaaa cgtaagcacc gtttccattt tcccgccttc
     1921 tttcagcaac gtcgaattca tctgcccgtt gtgaatgaaa ggagcgatct tcggcacaca
     1981 tttttttcgc acccacgttt tgttgcattg cgccactgta tcactcgcta ctgcaagcgt
     2041 gaaaagagaa gccagtaatg ttgcccgtag tttcattcgc atagtatcct ccgacacgga
     2101 ttcataaagc gtgccaaaaa agttaaacgg gaaaagcgat gtaaggtttc agttccgggt
     2161 gatgatgctg tcgcggataa ctgtcacacg ttcgaggata ttcttgaatt ccagtgcgtt
     2221 aagacttacc gggggaggag cgggatctgt tccgagcgtt gtcattccgg atttatcatc
     2281 tgtagaaact acagtcggct gatcgggatt tttcggtaat gcgtcgaaga gaactttgag
     2341 ttcgcggagc tggccggtaa cggattttac atcctcgttt ttttccctgc cgagcaatac
     2401 aagcagttta tccagcgaat accgttgttc aacgatgcgg acgcggatat cattgctgtt
     2461 tgtctgctcc gccactttgc aggcgaggta taatccttcc acccagccgc cggcgaccat
     2521 cagcgaggaa gcgtattctt ccttgttttc atgcagcagc gcatcacagt cccagtacga
     2581 atccgtgata atgctgagca cgctgtcgcg gttgttcatg tttgcctcga ggcggttctt
     2641 gcggtcggtg ctgaatgccg tggaaacgtg cagcccgtcg gccagctttc ccgtgcagtc
     2701 gagatagacc attgtttccg cgtgctggtc gaaaattccc gcgaacgcaa ggtcagcgcc
     2761 gtaaacgccg agattaagcg caagcgaagc ggtggtggaa tatttcgaag cgttctgcgg
     2821 aagattgagg aaatccttgt tgtatttcgc gccggcttct ttcagcagtc ctgccgtttc
     2881 gatcggcgac ggaacataga cgaacacatt ttccgctgaa acaacatcct ggttcacttc
     2941 gatggaagac gaatcatttc cgttatcgtt cgctcctccc ggtgagttgc tgcccgagca
     3001 ggcgatcagt gcaaacgaaa taacagggac gctgacaaaa gcagaaattc ttttcataat
     3061 gaagtctcaa atatacttct atgaaacggg aaaaggcaac aaatgattca cgcgcaggaa
     3121 tatatttctg aacattttca gcgcgatcag tgtgcgtcga gccagttgtt tcccgtgccg
     3181 attcccacct caacgggaac gtcgagctcg agcgcttttt tcatacaggt ttcaacaaca
     3241 ggtttcacat cgttcacttc ttccttggga acatcgaaca caagttcatc gtgcacctgc
     3301 agcagcagct ttgtgcggaa acggtgtttt tccagctcgc ggtgcacgtg tatcatggcc
     3361 agcttgatca tgtctgcggc ggaaccctgg atcggtgcgt tgatggcgtt tcgttcggaa
     3421 aaaccgcgca ccaccgcgtt cgcggaattg atgtcgcgca ggtaacgccg gcgcccgcag
     3481 atcgtctgca cgtagccatg ctcgcgtgcg aaattgatca tgttgtccat gtatttctgg
     3541 atgccgggaa attctttctt gtagttgtcg atgatctctt tcgcctccgc gcgcggaatg
     3601 cccaggttct cggcaagccc gaatgcgccc tgcccgtata taatgccgaa gttcacgctc
     3661 ttcgctttgt agcgcatttc tttcgtcacc tctttttcat ccacgccgta tacgcgtgcg
     3721 gcggttgccg tgtgaatgtc gacaccatcc ctgaatgctt tgcacatgtt cggatcgccg
     3781 ctgatggacg caacgatgcg cagttcgatc tgcgagtaat ccgcgctgag cagcacgtgg
     3841 tgatggtcgc gcgggatgaa agctttgcgt atctctcttc cccgcgccgt tcgtatcggg
     3901 atgttctgca ggttgggatt gtcggagctc agccgcccgg tggccgctac gacctggttg
     3961 aatgtcgtgt gaattctgcc ggttgccggg ttcaccattt ccggcagcgt gtcgacgtat
     4021 gtattcttca gcttcaccag ctcgcggtag gtgagtatct tttccacgat cgggtgctcg
     4081 tgcacgagtt tctgcagcac gtcctcgccc gtggcgtatt gtcctgtttt tgttttcttc
     4141 ggtttcccgc cgatcttcag cacttcgaaa aggatatcgc ccacctgctt cggcgaggaa
     4201 atgttgaacg gcgttccggc gagctgctgg atctctttgt cgacatcgac gatctcgttt
     4261 tccagctcgg cggaatattt tttcagcacg gcggtgtcga tggcgatgcc ttcattctcc
     4321 atgtcggcga gcacaggcac cagcggaatt tcgatatcgc ggaaaagctt ttcggcgccg
     4381 gcttctttca gctgcggttc aaaaacatcc tgcagctgcc acgtcacgtc ggcatcttcc
     4441 gcggcatatt ccgaaatttt ttccagcggg acatcgcgca tcgtcagctg gttcttcccc
     4501 cgtttcccga tcagcgtttc gatgctcacg ggcgaatagc cgaggtacgt ttcggaaagc
     4561 aggtccatgt tgtgacgcat gtccgggtga agcaggtaat gtgcgatcat cgtgtcgaag
     4621 agctcgccct gcacggaaat tttataccgg cgcagcacgt tcatgtcgaa cttgatgttc
     4681 tggcctgtct ttgcaatttt tttattctcg aaaacagcgc ggaattcttc catcacttcc
     4741 tgcgcctctt tttcttctgc aggaacggga acgtaccagc cttcatgttt tttccagctg
     4801 aacgacatgc cgacgatcga agccgtcatc gtgtcgagcc cggtggtttc cgtgtcgaag
     4861 caaaaggagg aaagcttttc caggtgagcg accagcgccg cccgtttttc cttcgtgtcc
     4921 gcgacatgat aatgatgcgt aacattctca atggttttga agctcgtgtt ttcgccggct
     4981 tccggtacgg aaggttcccc gcccgtattc ccgaatatgt ctatctgccc cggctttgtt
     5041 ttgcgcgatg ctgttttttc cgggttgccg ttttcttctt ctttttcagt ttcaccggcg
     5101 ggtaattctt cacccagcac ggtttgtgca aggcgccgga attccagctc gctgaaaagc
     5161 tgcttcactt tttcgcggtc cacttccttc agcttcagct gctcctcgtc cagctccacg
     5221 ggcgcatcgg tgatgatggt tgccagcttg cgcgagaaca gcgcctgttc ttttccctgc
     5281 tctacttttt ctttcagttt tcctttgagc ttgtcggtgt tcctgagcag gttgtcgatg
     5341 ctgccgaatt cgtggatgag ggaaagcgcg gttttttcac ccacgccctt gatgcccggg
     5401 atgttgtcga cgctgtcgcc catgagcccg aggatgtcga tcacctgttc gggatgcttg
     5461 atgccgtact tcgcacagat ctcttcgctg cccagttttt ccggtgcgcc gcccttgtag
     5521 ccgggtttgt aaatgaacgt gtgatgtgaa acgagctgcc cgtaatcctt gtcgggcgtc
     5581 atcatgtacg taatgaaatg ttttttctcc gccttcttcg ccagcgttcc gatcacgtcg
     5641 tcggcttcga acccgtccga ctccagcgtt gcgatgttga acgcatcgag
//
LOCUS       DASTSF010000633         2949 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_138623,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000633 DASTSF010000000
VERSION     DASTSF010000633.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2949)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2949)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2949
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138623"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..753
                     /locus_tag="VFU15_17775"
     CDS             <1..753
                     /locus_tag="VFU15_17775"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEU4719701.1"
                     /translation="VSVLLFTAAWFARDSAGAPPPAENFRASQKKIERVKNAYDLKWP
                     GIKKKLSEMKIDTSSFELFIRGFKEEKQIEVWVKNKNATTWSLYTTYAICASSGEAGP
                     KRCQGDGQVPEGFYHVNTFNPYSNYLLSLGVSYPNSSDKVFACRHDPGGAIMIHGNCV
                     TIGCIPITDDKIRELYVMAVEAKADGQEEIPIHLFPARMTKEKTDALKKSNPDPQTGI
                     FWDNLKEGYDKFEAAKTLPVITVNKAGKYIFG"
     gene            complement(840..>2949)
                     /gene="pbpC"
                     /locus_tag="VFU15_17780"
     CDS             complement(840..>2949)
                     /gene="pbpC"
                     /locus_tag="VFU15_17780"
                     /inference="COORDINATES: protein motif:HMM:TIGR02073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HEU4719702.1"
                     /translation="HPGVNPFALGRALKQDISSRRVVSGGSTLTMQVIRISRKNPPRN
                     LWQKFIEIILATRIEISYSKNEILAMYVSHAPFGSNVVGLDAASWRYFGREPEKLSWA
                     ESATLAVLPNSPALIYPGKNQLRLKEKRDHLLDRLFIAGIIDKNTCTLSKMEPLPGAP
                     HELPRLAPHLLVRASQEGNKGQVVQTTIDGNLQQRVTAIVDKHSSILRGNQVNNAAAL
                     VLDVETGNTLAYVGNSAKESDQDNGEQVDVITAPRSTGSILKPFLYAGMMSDGLILPN
                     TLVPDIPTDLSGYNPQNFNLSYDGAVPARRALARSLNVPAVRLLQQYHVDRFHELLKK
                     IGLTTITRPADDYGLSLILGGAEGTLWDLAGAYASMARTLTRYNKTGAYDKTDFHPPF
                     YMPQKNETHEPQVGEHSYFDASAIWFTFDAMTEVTRPDEEVNWTEFLSSNKIAWKTGT
                     SFGFRDGWAIGVTPKYVVAVWVGNADGVGRPGLTGINTAAPVLFDIFSMLRSPEWFAK
                     PESDMAKVMICKESGCRATDLCDKEEQWIPKSGLHSQACPYHVLVHLDHTGKFQVTSD
                     CEDPSDMQHVPWFVLPPTMEYYYKQKNPGYKTLPPFRKDCGGQPTHSMDFVYPREETQ
                     LYIPTELDGRPGKVVFEIAHRKPGTTVFWHLDDQYMGSTKGIHQFAMNPDPGEHKVTC
                     VDENGESISMKFTILNEKKK"
BASE COUNT          635 a          770 c          866 g          678 t
ORIGIN      
        1 gtttcggtac tgcttttcac ggctgcctgg tttgcccgtg acagtgcggg cgctccgccg
       61 ccggcagaaa atttccgcgc ttcgcagaaa aaaatagagc gcgtgaaaaa tgcctacgac
      121 ctgaaatggc cgggcataaa aaagaagctt tcggaaatga agatcgatac ttcgtctttc
      181 gagcttttca tccgcgggtt caaagaagaa aagcagatcg aggtctgggt gaagaacaaa
      241 aatgcaacga cctggtcgct ttacacgacc tacgcgatct gcgccagctc gggtgaggcg
      301 ggtcccaagc gctgccaggg cgacgggcag gtgccggaag gattttacca cgtgaacacg
      361 ttcaacccgt acagcaatta tttattgtcg ctcggcgtga gctaccccaa ttcatccgac
      421 aaggtttttg cctgcaggca cgatcccggc ggggcgatca tgatacacgg caactgcgtg
      481 acgatcggct gcattcccat tacggatgat aagatccggg agctgtacgt gatggcggtg
      541 gaagcgaaag cggacgggca ggaagaaatt cctattcatc tttttcccgc acgcatgaca
      601 aaggaaaaaa cagatgcgct gaaaaaaagc aatcccgatc cgcagaccgg gatcttctgg
      661 gataacctga aagaaggtta tgataagttc gaagcagcga aaacgttgcc ggtgatcacg
      721 gtaaataaag ccgggaaata tattttcgga taaggagtgt tgtttttatc tcaaactgca
      781 ggaagcgaaa acgcatagcg tacaaaggaa agcggcgtgc cagttaccgg actgcagaat
      841 tattttttct tttcattcag tatcgtaaac ttcatcgaaa tgctttcccc gttctcatcc
      901 acgcaggtca ctttgtgttc tccgggatcg gggttcatgg caaactggtg aatgcctttg
      961 gtcgatccca tgtactggtc gtcgaggtgc cagaaaacag tggtgccggg cttgcggtgc
     1021 gcgatttcaa aaacaacttt tccgggccgg ccatcgagct ccgtcggaat gtagagctgt
     1081 gtttcttcgc gcgggtaaac gaagtccatg gaatgcgtgg gctggccgcc acagtctttg
     1141 cggaaaggcg gcagcgtttt gtagcccggg tttttctgtt tgtaataata ttccatggtt
     1201 ggcggaagga cgaaccaggg aacgtgctgc atgtccgaag gatcttcgca atcgctggtc
     1261 acctggaatt tcccggtgtg atcgagatgc acgagcacat gatacgggca cgcctgcgaa
     1321 tgcaaaccgc ttttcggaat ccattgctct tccttatcgc aaaggtccgt tgcgcggcag
     1381 ccgctttctt tgcagatcat cactttcgcc atgtcgctct ccggcttcgc gaaccattcg
     1441 ggcgaacgca gcatgctgaa aatgtcgaac aataccggcg ccgccgtgtt gatgcccgtg
     1501 agaccgggcc gccccacgcc gtcggcattt cccacccaaa ccgcgacgac atatttcggc
     1561 gtcacgccga tcgcccaccc gtcgcggaaa ccgaagctgg tccctgtttt ccacgcgatc
     1621 ttgttcgacg acaaaaattc cgtccagttc acttcttcat cgggacgcgt cacttccgtc
     1681 atcgcatcga acgtgaacca gatcgctgac gcatcgaaat aggaatgctc tcccacctgc
     1741 ggctcgtgcg tttcattttt ctgcggcatg taaaacggcg gatggaaatc ggttttgtcg
     1801 tacgccccgg ttttgttgta acgggtaagc gtgcgcgcca tcgatgcata cgcaccggcg
     1861 aggtcccaca atgttccttc tgcgccgccg agaatgagcg agagcccgta atcgtctgcc
     1921 ggccgcgtga tggtggtgag cccgatcttt ttcagcagtt cgtggaaacg gtcgacgtga
     1981 tattgctgga gcaaacgcac ggccgggacg ttcagcgatc gtgccagcgc acgtcgtgcg
     2041 ggaacggcgc cgtcgtacga aagattgaaa ttctgtgggt tgtagccgga aagatccgtg
     2101 ggaatatcgg gcacgagcgt attgggaagg atcagcccgt cgctcatcat gccggcgtac
     2161 aggaagggtt tgagtatgct gcccgtgctc cttggtgcgg tgatgacgtc gacctgctcg
     2221 ccattgtcct ggtcgctttc ctttgcggaa ttaccgacgt acgccagcgt gttccctgtt
     2281 tccacgtcga gcacgagcgc agcggcgttg ttcacctggt tgccgcggag aatgctggaa
     2341 tgcttgtcga caatggcggt gacgcgctgc tgcaggttcc cgtcgatggt ggtctgcacg
     2401 acctgtccct tgtttccttc ctgcgaagcg cgtacgagca ggtggggagc gaggcggggc
     2461 agctcatgcg gtgcgcccgg taacggttcc attttggaaa gcgtgcacgt gtttttatcg
     2521 ataatgccgg cgatgaacag gcggtcgaga agatggtcgc gtttttcttt tagccggagc
     2581 tgattttttc ccgggtagat gagcgcagga ctgttcggga gaacagcgag cgtagcggat
     2641 tccgcccagg aaagtttttc gggttctcgt ccgaaatagc gccaggaggc cgcgtcgagt
     2701 cccacgacat tgctgccgaa cggtgcgtgc gaaacgtaca tcgcgaggat ttcattcttg
     2761 gaataggaaa tttcaatccg cgtggcgagg atgatctcga tgaatttctg ccagaggttg
     2821 cgcggcggat ttttccgcga gatgcggatc acctgcatgg tgagcgtgct gccgccggaa
     2881 acaaccctgc ggctggaaat atcctgcttc agcgcacgtc ccagcgcgaa cgggttgacg
     2941 cccgggtga
//
LOCUS       DASTSF010000634        12176 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate SMAG_U2273 k141_1031973,
            whole genome shotgun sequence.
ACCESSION   DASTSF010000634 DASTSF010000000
VERSION     DASTSF010000634.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960737
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (soil metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 12176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:29:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,567
            CDSs (total)                      :: 3,537
            Genes (coding)                    :: 3,535
            CDSs (with protein)               :: 3,535
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12176
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1031973"
                     /isolate="SMAG_U2273"
                     /isolation_source="soil"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            307..1395
                     /locus_tag="VFU15_17785"
     CDS             307..1395
                     /locus_tag="VFU15_17785"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HEU4719703.1"
                     /translation="MQVVRFISADPTEKQFAAAVRKNVGDYFREKGISTKGNGWMYVK
                     AAVLLGTYIAPFVVLLTVPVGWPLALLLVFLMGIGEAGVGMSVMHDAVHGSFSAKKSV
                     NELFSWSMFLLGSNIFNWKVQHNILHHTFTNIYGYDEDIETKAVIRLCDHAPRKKFHR
                     FQLIYAFFFYGLMTLSKLTTDFSQLIAYNRRGITREQKRNPSAEMLKLIAGKLAYLFI
                     ILGFPLLFTSFTWWQILTGFAILHITAGMIMSTIFQMAHVVEGTEQPVPDEKGVIAHD
                     WVVHEMRTTSDFAPKNHFLNWYAGGLNFQIEHHLFPNTCHIHYRKIAPIVEQTAQEFG
                     IAYNRKPTFGAALVSHLKRLKELGKQPS"
     gene            1697..1885
                     /locus_tag="VFU15_17790"
     CDS             1697..1885
                     /locus_tag="VFU15_17790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006258143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HEU4719704.1"
                     /translation="MQNGTVKFFNQTKGFGFIKTENGEEVFVHVTGLKEEVRDNDEVV
                     FDLEEGRKGLNAVNVRLA"
     gene            1954..2817
                     /locus_tag="VFU15_17795"
     CDS             1954..2817
                     /locus_tag="VFU15_17795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HEU4719705.1"
                     /translation="MRQLTIGKSITTRDSASLDKYLSDISKIPMITVEEETILAQKIR
                     SGDEDSLEKLTKANLRFVVSVAKQYQNQGLSLSDLINEGNVGLIKAARRFDETRGFKF
                     ISYAVWWIRQSIIQAVSEQSRVIRLPLNKIGTMKKIGKMQSQLEQKLERPASVSELAE
                     AMEISEEEISATMGAAVRHISTDALLPGNEDLTIMDVTSDPGNSLPDDLLILSSLKTE
                     ISRALATLTERERDVITLYYGIGRPGAMTLDEIGEKHELTRERVRQIKEKAIRRLRHV
                     TRSKVLKTYLG"
     gene            complement(3118..3206)
                     /locus_tag="VFU15_17800"
     tRNA            complement(3118..3206)
                     /locus_tag="VFU15_17800"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3170..3172),aa:Ser,seq:gct)
     gene            3365..5245
                     /locus_tag="VFU15_17805"
     CDS             3365..5245
                     /locus_tag="VFU15_17805"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU4719706.1"
                     /translation="MKFSRFSFLLAAPVLLLFSCSDQPQGKFTENKKGDTASNNIGVK
                     FVHNPSTTQWQKDWSTDNVVVYHWRAEPDNLHPSNGQSNPRRVVMDYTQRFLVSTDFE
                     KQSIRPDLIKSMPTVSPDGLRYDYELRDEPVWDDGTPLTADDVEFSMKAFLCPYTEDG
                     FAKPYFETLAGFEKDASNPKKFTVICSRKYIQNVALFGDLTIMERKYYDPKNVLGNYS
                     LAQMNDPAFVKTMHADLKTWADEFNSPKYGTDPQSMQGLGAYKVQQWDKGQRIILVKK
                     KNHWTSKVQNPNMYDAAYPDQIIFQINTDDNSIALQLKNQKIDVSTWIQTHSLIELQQ
                     DTNFNRNYFSAFVPNFNYQYLGMNLKPEASNRLPFFTDKRVRHAMALLIPADQMNQAY
                     LDGKAIRMTSLVSPMKPDIYNTSLKPVPFNIDSAKTLLDEAGWKDSDNDNIRDKMIDG
                     KKVPFKFELLIMTGNPVVPKMANDIKDAMHKAGIEVTITSLEFGAFYNQVMAHQFDMY
                     MGAWASASGPEDYKQIWSSTSWENKGSNYVGFGTPATDSLIEKIRVTIDDSARTPMIK
                     QLQQMVYDEEPYVFLYSVPSKIAIHRRFDHADMYYDKPGVFLSNLKLLTPGNMMKPAP
                     VQ"
     gene            5263..6798
                     /locus_tag="VFU15_17810"
     CDS             5263..6798
                     /locus_tag="VFU15_17810"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU4719707.1"
                     /translation="MLRYILRRVLIFIPTLFAISIIAFIISINAPGDPIDVLVQGGNQ
                     GSDAGNVMNRNLEQRKDSIRRKLGLDLPVFYFSFSNIAVPDTLYRIFDQNEHEAASRL
                     INIYGNWDEISAWMKSVKAMQHSLDNIAPDSIAQQRLGRDSANQLLDITRQDMMSLLA
                     TYDEVVISSRLKKISAGEQALVSLGEHNFLGAAQKPIEQSRAAFDAMRSHATKWKNYV
                     PSIKCYGYNQYQRWLFGDGNWLTGKNAVNTKGVIRGDFGKTYTSMLSEPVSKTISRAL
                     PWSLFFTVTSVLFAYLVSIPIGVQAAANRGGFFDRTSSVVLFMLFSLPSFFMATLLLM
                     TFANPDVLNIFEAGGVEPAAGIPHGISLWAKLKIELPFLVLPFICYTYSSLAFLSRTM
                     RVALLEILNQDYMRTARAKGLSKFNVIYRHGLRNALLPIITVFANIFPAAVGGSVILE
                     FIFTIPGMGKEIIEAIYTKDYPMIVCVFTLTGVMTLLGYLISDILYAVADPRISYSKN
                     SKG"
     gene            6795..8162
                     /locus_tag="VFU15_17815"
     CDS             6795..8162
                     /locus_tag="VFU15_17815"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU4719708.1"
                     /translation="MSEEVKNADNRPEEKKDPVNFSFRRHTWKQFKKNKPALVSFYIL
                     IFLLAVAVFAPVIANERPLYMKYEGQTFYPAFSFKSNYIFTNPATGHQENIQLDEADW
                     KRMNAESVVWAPIAWSPSVKDPDNQNYVGPGDPQKFIDAKGDTLFMPSRFRHLLGTTS
                     EGKDLLAGLIHGTRVSLSIGFISMGIASLLGLFLGAVAGYFGDERLQTKRGTFWMTLL
                     GLPFAWFYAFSVRSYALRDAMADSGYMFLFQLLVSIFIFAAICRLFSFLGKLISRGTF
                     LGKKVFFPVDSFVSRGIEILNSIPTLILIISLAALVKEKSMIYVMVIIGLTSWTGIAR
                     FTRAEFLRIRTMDYIQAAQAMGFKERRIIFRHALVNGMAPSLVSIAFGIAAAILIESS
                     LSFLNVGVPDDLQTWGKLLFEGKERFHAWWMIIYPGLCIFLTVTVYNLIGEGLRDALD
                     PKLKK"
     gene            complement(8511..8966)
                     /locus_tag="VFU15_17820"
     CDS             complement(8511..8966)
                     /locus_tag="VFU15_17820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4719709.1"
                     /translation="MKKSNSLFSFLTIVLPVLAIGVWMLLSPQGEAHQEIEDAGVYFV
                     KSIWGKPAGLTILVFGIFLAVVLIITSRRGQQGGLSPGRLSFRISFWIFTCTAVIYAI
                     ILVQNFLVETGMLGKDFYFTAMDDIALVIYTFLVVFGITAGILWLVLKK"
     gene            complement(9006..9803)
                     /locus_tag="VFU15_17825"
     CDS             complement(9006..9803)
                     /locus_tag="VFU15_17825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEU4719710.1"
                     /translation="MKRALFLLIAFAALSSWSCSRVPITNRRQFNLIPEVEMQSLSLT
                     AYSDFMKSHTVMPANDSGSILVKKVGKKISDAIVKYLNDHHQGKRVAGYQWEFNLVKS
                     DEVNAWCMPGGKVVVYTGLLPVTKDEDGLAVVLGHEIAHAIARHGNERMSQQLAIQMG
                     GMALAVAVSQKPEQTQQIFLQCFGVGTTLGALAYSRKHESEADKLGLCFMGMAGYDPN
                     KAVPFWERMSKLGNGAQIALLSTHPTDAQRIKDIKTFMPTALKYYKK"
     gene            complement(9842..10129)
                     /locus_tag="VFU15_17830"
     CDS             complement(9842..10129)
                     /locus_tag="VFU15_17830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009195394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HEU4719711.1"
                     /translation="MSFRFSHQTEKEEVLLLEEELEDLLDLVIYNDDFNTFEFVIETL
                     MKVCRHDLIQAEQCTMIIHNNGKCGVKRGTAEELQPMCEAILERGISAKIE"
     gene            complement(10402..>12176)
                     /locus_tag="VFU15_17835"
     CDS             complement(10402..>12176)
                     /locus_tag="VFU15_17835"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEU4719712.1"
                     /translation="IASADNPPVYSNARVADNSNGNNSGNNGNSNSNVADNNNGNNSG
                     NNNNSNSNVADNDNGNNSGNNNNSNSNVADNNNGNNSGNNNNSNSNAADNTNGTNSGN
                     NANNSNSNVADAANNPNSIVHHLAPGEKLDFSPSATNPPSASAPVDTSGLVYKVQIGA
                     FRNAIPTDLFKGIDPVTYEKTASGLTRYTAGYFVVFANADAAKNEIRKMGYPDAFVVA
                     YYNGKRVSMDEANRIAGGGKPGNPQNNHVADNNNAGNANPGSNGNNAGNNGNTNNGSN
                     PSQNTNNNYVPGSNSPGNVAKATDVATVKDLFYTVQVGVFSKQVSARQLKNLDGLFYE
                     QTASGTYRYSSGKYNNMDAAVAAKNAIVSKGISDAFVTAYYDGKRITTDEAKTLQASG
                     TQAAGDSQSNVQFTNNGYVPQNNSQQNNNTPPADNGNNNSAAANTPPHSGNPGYSNVP
                     HDNPNAGNNNNSNSNNNNNNNNNNNNNNNNTNTGTDGNVTPADNAKIVPDTGLVFSVQ
                     LGAFKDEVPLDIATKFLQFASRGVKHYVDPSNGYTVYQVGVFTTKDEADKLRDEAAAK
                     GINDAFVVAWKDGKKISMDEAMGN"
BASE COUNT         2969 a         3170 c         3000 g         3037 t
ORIGIN      
        1 ccggcgactt ccacgtaata cgcgaaggtc tgggcgggac ggagaaccta tggtaacatc
       61 ccaccggaaa atccgcgtaa tccccgcaca aggtcgtttt ctgaaatgct gttttcgttt
      121 ataaaaaaca cattttcaca aaccggcatt tctccattat cctgtttcat tcaggaacgc
      181 cgcagccgaa aaccacaagg cttctgacca aaacttcacg tttccgtgac cgggatcatc
      241 ccgaaaaaaa cttccgtgtc aaaactttgc tggccggaaa caggattttt ctgatcgtaa
      301 aacagcatgc aggtcgtcag gttcataagc gcagatccca cggaaaaaca gtttgcagct
      361 gctgtccgga aaaacgtcgg cgattatttc cgggaaaaag gaatttccac caaaggaaac
      421 gggtggatgt atgtaaaggc ggctgtgtta ctcggaactt atatcgcacc gttcgttgtt
      481 cttttaactg ttccggtggg ctggccgctc gcattactgc tcgttttcct gatgggaatc
      541 ggtgaggcgg gcgtaggcat gtcggtgatg catgacgctg tgcacggttc cttttccgcg
      601 aaaaaaagtg tgaacgagct gttctcctgg tcgatgttcc tgctcggcag caacatattc
      661 aactggaaag tgcagcataa catcctgcat catacgttca ccaatattta cgggtatgac
      721 gaagacatcg aaacaaaagc ggtgatccgt ttgtgcgatc atgcgccgcg gaaaaaattt
      781 caccgtttcc agctcattta tgcgttcttc ttttacgggc tgatgacttt gtcgaagctc
      841 accaccgatt tcagccagct gatcgcatac aacaggcggg gcatcacgcg tgagcagaaa
      901 cgaaatccct cagcggaaat gctgaagctg atcgcgggca agctcgctta tctttttatc
      961 attctcgggt tcccgctgct cttcacttct ttcacgtggt ggcagatact taccggtttt
     1021 gccatccttc acatcacggc gggaatgatc atgagcacca ttttccagat ggcgcatgtg
     1081 gtggaaggaa cagagcagcc tgttcccgac gagaaaggcg tgatcgcgca cgactgggta
     1141 gtgcatgaaa tgcggaccac ttccgatttc gcaccgaaga atcatttcct gaactggtat
     1201 gccggcggac tgaatttcca gatcgagcat cacctgttcc cgaacacgtg ccacattcat
     1261 taccggaaaa tcgccccgat cgtcgaacag acagcgcagg aattcgggat tgcgtacaac
     1321 cggaaaccga cattcggcgc agcgctcgtt tcacacctga aacgtttgaa agagctggga
     1381 aaacaaccat cataacgcag cgcggcgaaa tatatctcgg cccggacgtg gaatccggct
     1441 tcaaaagccg tatctttgac tacttcatca gaagattgta tctcacgctg cctgcagcta
     1501 tccggttgca gctttgtaaa ttttacactt ttcttatcaa gcaggaagca cgactttgga
     1561 tttccggagt ctgcccggtt ttgcgcatac cagttcaacg gcaatcacag gcagtacccc
     1621 cgacagactt atctcttcct tgattatttc atccaatcat taaccattcc cgtacaaccg
     1681 gggtaaaaca aaaacaatgc aaaacggtac agtaaaattt ttcaaccaga caaaagggtt
     1741 cgggttcatc aaaacagaaa acggtgaaga agtattcgtg catgtcacag gtcttaaaga
     1801 agaagtccgt gacaacgacg aagtggtgtt cgatctcgaa gaaggcagaa aaggcctcaa
     1861 cgcggtgaac gtgcgcctgg cgtaagccgc ctcgtcacac gaaacagttc tgcggaactt
     1921 ccgctgtcat tccacaacgt taaacatcga tgcatgagac aacttacaat cggtaaatcg
     1981 attaccacca gggactcggc atcactggat aaatatctct ccgatatttc aaagatcccg
     2041 atgatcaccg ttgaggaaga aaccatcctg gcgcagaaaa tacgttcggg cgacgaagat
     2101 tcgcttgaaa agctgacaaa agcaaacctg cgcttcgtgg tttcggtggc caagcaatac
     2161 cagaaccagg gactcagtct ttcggacctg atcaacgaag ggaacgttgg actgatcaaa
     2221 gccgcgcggc gcttcgatga gacgcgcggc ttcaaattta tttcctacgc tgtgtggtgg
     2281 atacgccagt cgatcatcca ggctgtttcc gaacagtcgc gggtgatccg gcttccgctc
     2341 aacaagatcg ggacgatgaa aaagatcgga aagatgcaga gccagctcga gcaaaagctg
     2401 gagcggcctg ccagcgtatc cgagctggcg gaggcaatgg aaatttccga agaagaaata
     2461 tcagcgacga tgggcgccgc ggtgagacac atttcaaccg atgcgctcct gccgggaaat
     2521 gaagacctca cgatcatgga cgttacgtcc gatcccggca attcacttcc ggatgatctg
     2581 ctcatcctgt catcgctgaa aacagagatc agccgcgccc tcgccacgct tacggaaaga
     2641 gaacgcgatg tgatcacttt gtattacggt atcggccgtc cgggtgcgat gacgctcgac
     2701 gagatcggtg aaaagcacga gcttacgcgg gaacgcgtga ggcagatcaa agaaaaagcg
     2761 atacggagac tgcgccacgt tacgcgcagc aaagtgctga agacctacct cggctgatcc
     2821 gggcaatctg aaatttttgt gcgtgttaac aaaagccagc ggctttttgt ttttcattgc
     2881 gcgggttcaa acaggtttgc gtccgcattc aaaataaaac cccgccaatg cggggctttt
     2941 tattttttca tcggtgaagg agggattact cacgtgatcc cgttgggcga ggggcctaaa
     3001 acaaaatgcc tacggacttc gtcctgcaca tttttcattt ccattcgccg gtgaaagcaa
     3061 gctttcccgt tgtcctgaaa tgaaaaatgc cgcttcgctt gcggcatttt agttttgcgg
     3121 tgagggaggg attcgaaccc tcggtacagt ttgacccgta cggcagttta gcaaactact
     3181 ggtttcggcc actcacccac ctcacctgac ttgtggattt tgccgggcga caaaggtaaa
     3241 aaaagttttg ttccggggga gcggcccgat cgcctattta aattccggtg ttttcttttt
     3301 gtcagccatt tgctaagacg cagatgttat atttgcagga caacctaaag caaggctcct
     3361 cattatgaag ttttcccggt tttcctttct tcttgccgcc cccgttcttc tcctcttttc
     3421 ctgcagtgat cagccgcagg ggaaatttac cgaaaacaaa aaaggcgata ccgcttcgaa
     3481 caatattggt gtgaaattcg ttcacaatcc gtccactacc caatggcaaa aagactggtc
     3541 gaccgataat gttgtggtct accactggcg cgcggagccg gacaaccttc acccgagcaa
     3601 cgggcagtcg aatccgcggc gcgtggtcat ggactacacg caacgtttcc tggttagcac
     3661 cgatttcgaa aaacagtcca tacgtcccga tcttattaag tcaatgccca ccgtttctcc
     3721 cgacggtctg cgctacgatt atgaattgag agacgaacct gtatgggacg acggtacgcc
     3781 gcttaccgcg gatgatgttg aattttcgat gaaggcgttc ctctgcccgt atacggagga
     3841 tggttttgcg aaaccgtatt ttgaaacgct cgcaggattt gagaaggatg cttccaatcc
     3901 gaagaagttc accgtgatct gtagccggaa atacatacag aatgtggctc ttttcggcga
     3961 cctgacgatc atggaaagga aatattacga tccgaaaaac gtgctgggca actattcttt
     4021 ggcgcagatg aatgatcctg cttttgtaaa aaccatgcat gctgacctga aaacatgggc
     4081 ggacgaattc aattcaccga aatacggaac ggatccgcaa tccatgcaag gactcggcgc
     4141 atacaaagtt cagcaatggg acaaaggcca gcggatcatt cttgtcaaaa agaaaaacca
     4201 ctggacctcg aaagtacaga acccgaacat gtacgatgcg gcttatcccg atcagatcat
     4261 tttccagatc aataccgatg ataattccat cgcgctccag ctgaaaaatc agaagatcga
     4321 cgtttccacg tggatacaga cgcacagcct catcgaattg cagcaggaca cgaatttcaa
     4381 ccgcaattat ttttcggcct tcgttcccaa tttcaattac caatacctgg gtatgaacct
     4441 gaagccggaa gcgtccaacc gtttgccttt tttcaccgac aaacgcgtgc gtcatgcgat
     4501 ggcgttgctt atcccggccg accagatgaa ccaggcttac ctcgacggaa aggcgatccg
     4561 catgacgagc ctcgtttccc ctatgaaacc cgacatctac aatacgagcc ttaagccggt
     4621 tccgttcaac atcgattcgg cgaaaacact cctcgacgaa gcaggctgga aagattcgga
     4681 taacgacaat atccgcgaca aaatgatcga cggaaaaaaa gtgcctttca aattcgagct
     4741 gctcattatg acgggaaatc ctgtcgttcc gaaaatggcg aatgacatca aggatgccat
     4801 gcacaaagcg ggtatcgaag tgacgatcac ctcgctcgag ttcggcgctt tctacaacca
     4861 ggtcatggcg caccagttcg acatgtacat gggtgcatgg gcatcagcat cgggaccgga
     4921 ggattacaag cagatatggt cttccacgtc atgggaaaac aaaggatcga attatgtggg
     4981 attcgggaca cccgcgaccg attcgcttat cgaaaagatc cgtgtaacga tagacgattc
     5041 cgcacgcaca ccaatgatca aacagcttca gcagatggtg tacgatgaag aaccttacgt
     5101 cttcctttat tccgttccat cgaagatcgc catacaccgc cggtttgacc atgcggatat
     5161 gtactacgac aaacccggtg ttttcctcag caatctcaag ctgctcactc ccggcaacat
     5221 gatgaagccg gcaccggtac agtgaaacaa cctgcatgac cgatgctgag atatatttta
     5281 cgcagggtat tgattttcat tcctacgctt ttcgcgattt cgatcattgc gttcatcatc
     5341 agcatcaacg caccgggtga tcccatcgat gtgctcgttc agggaggaaa ccagggcagc
     5401 gatgccggga acgtgatgaa ccgtaacctc gagcagcgta aagattctat ccgcaggaaa
     5461 ctcggcctcg accttccggt tttttatttt tcgttcagca acatcgccgt tccggacacg
     5521 ctttaccgca tcttcgatca gaacgaacat gaagcggctt cacggcttat caatatttac
     5581 gggaactggg acgagatcag cgcgtggatg aaatccgtga aggcgatgca gcattcgctg
     5641 gacaatattg cgcccgacag tatagcgcag cagcgcctcg gccgcgactc cgcgaaccag
     5701 ctgcttgaca tcacccgcca ggacatgatg tcgctgctgg cgacctatga cgaagtggtg
     5761 atctcgtcgc gcctgaaaaa aataagcgca ggcgagcagg cgctcgtttc gctgggtgaa
     5821 cataatttcc tcggagcggc gcaaaaaccg atcgaacaaa gcagggctgc atttgatgcc
     5881 atgcggtcgc atgcgacaaa gtggaaaaat tatgtgccgt cgatcaaatg ctacgggtac
     5941 aaccaatacc agcgatggct tttcggtgac gggaactggc tcaccgggaa aaatgccgtc
     6001 aacaccaaag gcgttatccg cggcgatttc gggaagacgt acacgtccat gctttcagag
     6061 cccgtatcca aaacgatcag ccgggcgctg ccctggtcgc tgttctttac cgtcacttcg
     6121 gtgctcttcg cctacctcgt cagcatcccg atcggcgtgc aggcagctgc gaaccgcggc
     6181 ggatttttcg accggacatc ctccgttgtg ctcttcatgc tgttctcgct tccttctttt
     6241 ttcatggcca cgctcctgct catgaccttt gccaacccgg atgtgctgaa cattttcgag
     6301 gcgggcggcg ttgaaccggc ggctggaatt ccccacggaa tttcgctgtg ggcgaagctg
     6361 aagatcgagc tgcccttcct cgttcttccg ttcatctgct acacgtacag ctcgctcgct
     6421 tttctcagcc ggacgatgcg cgttgcgctg ctggagatac tcaaccagga ttacatgcgc
     6481 acagcgagag caaaaggttt gtctaagttc aacgtgatct acaggcatgg attacgtaac
     6541 gccctgctgc cgatcatcac cgtattcgcg aatatttttc cggccgcggt cggcggatcc
     6601 gttatcctcg aattcatttt caccattccc ggcatgggaa aggaaatcat cgaagcaatc
     6661 tacaccaaag attacccgat gatcgtctgc gtatttacgc tgaccggtgt aatgacgctg
     6721 ctcggttatc ttatttcgga tatcctttac gccgtggccg atccgcggat atcctattca
     6781 aaaaacagca aaggatgagc gaggaagtga aaaacgcgga caaccggccg gaggaaaaga
     6841 aagaccccgt gaatttcagc tttcgccggc acacgtggaa gcagttcaaa aagaacaagc
     6901 ccgcgctcgt ttcgttttac atcctgattt tccttcttgc ggtagccgtc ttcgccccgg
     6961 tgatcgcgaa tgaaaggccg ctttacatga agtacgaagg ccagacgttc tatcccgcgt
     7021 tttcgttcaa atcgaattac atctttacca acccggcgac cggccaccag gaaaacatcc
     7081 agctcgacga ggcggactgg aaacggatga atgccgaatc ggttgtgtgg gcacccatag
     7141 cctggtctcc ttctgtaaaa gacccggaca atcaaaatta tgtcggaccg ggtgatccgc
     7201 agaaattcat cgatgcaaaa ggcgacacgt tattcatgcc atcccgtttc cgtcacctgc
     7261 tgggaacaac atcggaagga aaagacctgc tcgcaggatt gatccacgga acgcgcgttt
     7321 cgctgagcat cggttttatc tcgatgggaa tcgcgtcatt gctcgggctt ttcctcggcg
     7381 cggttgcagg ctatttcggt gacgaaagac tgcagacgaa aaggggcaca ttctggatga
     7441 cattgctcgg actgccgttc gcctggttct atgcgttcag cgttcgcagc tatgcattgc
     7501 gtgatgcgat ggcggattcc ggatacatgt tcctgttcca gttgctggtg agcattttca
     7561 ttttcgcggc gatctgccgg ctgttctcgt ttctcggaaa gctgatcagc cgcggtactt
     7621 ttttaggaaa gaaagttttc tttcccgtcg attctttcgt ttcacggggc attgaaattc
     7681 tgaattcgat cccgacgctg atccttatta tttcgcttgc agcgctggta aaggaaaaat
     7741 cgatgatcta tgtaatggtg atcatcgggc tcacttcgtg gacagggatc gcgcgtttta
     7801 cacgtgcgga atttctccgt atacgaacga tggactatat ccaggctgca caagccatgg
     7861 gattcaagga aagacggatt attttccggc acgcgctcgt gaacggcatg gcaccgtcgc
     7921 tggtgtccat tgcgttcggc atcgcagcgg ccatacttat tgagtccagc ctctccttcc
     7981 ttaatgtcgg cgttccggat gatctgcaga cctggggaaa attattgttc gagggtaaag
     8041 aacgtttcca cgcctggtgg atgatcattt acccggggct gtgcatattc ctcaccgtta
     8101 ccgtttacaa cctcatcggt gaaggactgc gcgatgcgct ggatccgaag ctgaagaagt
     8161 aaagaaccgg gtccgcaata catattttac tgctttcgca gggatgccgc tttccggtga
     8221 tgcaattttt cagcgaccag aaataaattt tccattatgg ctgttccatc tgaacacatc
     8281 aaccctgctt tgagaaagca gacggtaaca gttcgcctta tctttttgtc agcagcctga
     8341 agttgtcaat gatttttacc gtcattgtac aggtccttca tgtccctgag gcataaacct
     8401 caaacaagca ttgatcaacc ggatctttgc aagatgtcaa tgcaggcatg cgcataaatt
     8461 cacttcttcc tgcggcgttt ccaaaaccac agtattgccc gaccgctccg tcacttcttc
     8521 agaaccagcc acagaattcc cgccgttatc ccgaaaacaa caaggaaggt atagatcaca
     8581 agtgcgatat catccatagc cgtgaaataa aaatcctttc cgagcatacc ggtttccacg
     8641 agaaaattct gaacgaggat gatcgcataa ataacagcgg tgcaggtaaa aatccagaac
     8701 gagatgcgaa atgaaagccg gcccggtgaa agtcctccct gctgcccccg ccgcgaggtg
     8761 atgatcagta caacggcgag aaatattccg aagacgagaa tggtgaggcc cgccggtttt
     8821 ccccagatgc ttttcacgaa ataaacaccc gcatcctcga tttcctggtg cgcttcaccc
     8881 tgcggcgaca ggagcatcca taccccgatc gccagcaccg gcaaaacgat ggtaagaaaa
     8941 ctgaaaagtg aattgctctt tttcaagcga caatcatttc gaattgcgaa ccgggaaagc
     9001 cacgcctact tcttgtaata cttcagcgcc gtcggcatga acgttttgat gtccttgatg
     9061 cgctgcgcat cggtgggatg tgtgctgagc aatgcgatct gcgcgccatt gccgagtttc
     9121 gacatgcgct cccaaaacgg gaccgctttg ttcggatcgt aaccggccat gcccatgaag
     9181 caaagcccca gcttgtcggc ttccgattcg tgcttgcgcg aataagcaag cgcgccgagc
     9241 gttgtgccga caccgaaaca ctgcaggaat atctgctgcg tctgttccgg tttttgcgaa
     9301 acggccacgg cgagtgccat tccacccatc tgtatcgcga gctgctggct catgcgttca
     9361 ttcccgtggc gcgcgattgc atgtgcgatc tcgtgtccga gcaccacggc gagtccatct
     9421 tcgtctttcg tcaccgggag aagcccggtg taaacaacca ccttcccgcc gggcatgcac
     9481 caggcgttca cttcgtccga tttcacgagg ttgaattccc attggtagcc ggccacacgt
     9541 tttccctggt gatgatcgtt gaggtatttc acgatcgcat ctgaaatttt cttgccaacc
     9601 tttttcacga ggatcgagcc cgaatcattc gccggcatca ccgtatgcga tttcataaaa
     9661 tcactgtacg ccgtgagact gagactttgc atttccacct cgggaatgag gttgaactga
     9721 cgacggttgg taatgggaac acggctgcac gaccaggatg acaacgccgc aaaagcgatg
     9781 agaagaaaaa gggcccgctt cataatgatc agaattggta atgtaaaaga tgggccatgc
     9841 cttattctat tttcgcggag atgcctcttt cgaggatcgc ttcacacatg ggttgcaatt
     9901 cctctgccgt tccgcgcttc acaccgcatt tgccgttatt gtgaatgatc atcgtgcact
     9961 gttcggcctg gataagatcg tgccggcaca ctttcatgag cgtttcgatc acgaactcga
    10021 acgtattgaa atcgtcattg tagataacaa gatccaaaag atcttcaagc tcttcctcaa
    10081 gaagaagaac ttcttctttt tctgtctgat gactgaaccg gaatgacatg gcttatttat
    10141 ttgtacaaag ttacttcgaa aattccggaa ttccgttgac aataccccgg caacggatgc
    10201 caaactggaa aggtggaact gttctcgact tgcgctcgaa ctgacggact ttgctcgaac
    10261 tgttctcgac tagcgctcga actgacgggt gacttgcgcc cgaactggca aatgactttt
    10321 gcgttatggc aaactgaaat caggtaactg aaaacgattc aaatccctgg cacgaaacca
    10381 caacccttca acctccccgg atcagtttcc catcgcctcg tccatggaga tcttctttcc
    10441 gtccttccac gcaacaacaa acgcatcatt gattcctttc gccgcagctt catcgcgcag
    10501 cttgtccgct tcatcctttg tcgtgaaaac gcccacctgg taaacagtgt agccattcga
    10561 cggatcgacg taatgcttca caccgcggct ggcaaactgc aggaatttcg tagcgatatc
    10621 cagcggaact tcatccttga acgcgccgag ctgaacggag aaaacaagac ctgtatcggg
    10681 aacgatcttc gcattgtctg ccggagtaac attcccgtca gtacctgtat tggtgttatt
    10741 gttattgtta ttgttattgt tattgttatt gttattgtta ttgctattgc tgttattgtt
    10801 gttgcctgca ttcggattgt catgcggaac attgctgtag ccgggattac cactatgcgg
    10861 aggcgtattc gcagctgcgg aattattatt tccgttgtcc gccggagggg tgttgttgtt
    10921 ctgctgagaa ttattctgcg ggacataccc gttgttcgtg aactgaacgt tgctctgcga
    10981 atcaccggcg gcctgcgtgc cggacgcctg cagggttttc gcttcgtccg tagtgatgcg
    11041 ctttccgtcg taatacgctg ttacaaacgc atcggaaata ccttttgaaa cgatcgcatt
    11101 cttcgccgcg accgccgcat ccatgttgtt gtatttcccg gatgaataac ggtacgtgcc
    11161 ggatgcagtt tgctcataga aaagtccgtc gaggtttttc agctgcctgg ccgaaacctg
    11221 tttcgagaat acaccgacct gcacggtata gaaaagatct ttgacggtcg ccacatcggt
    11281 cgcttttgcc acgttaccgg gagaattgct gcccggcaca taattattgt tcgtgttctg
    11341 cgacgggttg ctgccattat tcgtgttgcc gttgttacct gcattatttc cgttgctgcc
    11401 aggatttgca tttccggcat tgttattatc cgctacgtga ttgttctgcg ggttgcccgg
    11461 ctttccaccg cctgcaatcc ggttcgcttc atccatggaa acgcgtttcc cgttgtaata
    11521 agcgaccacg aatgcatccg gataacccat ctttcgtatt tcgttcttcg ctgcatccgc
    11581 attggcaaaa acgacgaaat agcccgcggt gtaccgcgta agcccggatg ccgttttttc
    11641 atacgtgacg ggatcgatgc ctttgaaaag atccgtcggg attgcgttgc ggaatgcgcc
    11701 gatctgcact ttgtacacaa gaccggaagt atcgaccggt gcgctggccg aaggcggatt
    11761 ggtagctgaa ggagaaaagt ccagcttctc gcccggcgcc aggtgatgca cgatcgaatt
    11821 cggattgttc gcagcatccg cgacgttgct gttggaattg ttggcgttat ttcctgaatt
    11881 agtcccgttg gtattatccg cggcgttgct gttggaatta ttattgttcc cggaattatt
    11941 tccgttgttg ttatccgcga cgttactgtt ggaattgtta ttgttcccgg aattatttcc
    12001 attgtcgtta tccgcgacgt tactgttgga attgttattg ttcccggaat tatttccatt
    12061 gttgttatcc gcgacgttac tgttcgaatt gccgttattt cctgaattat tcccgttgct
    12121 gttatccgca acccttgcat tcgaataaac aggcggatta tcggcagacg cgatag
//
